LOCUS       DAPGWD010000001        76610 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPGWD010000001 DAPGWD010000000
VERSION     DAPGWD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.001"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..515
                     /gene="cmtB"
                     /locus_tag="RHX88_000001"
     CDS             72..515
                     /gene="cmtB"
                     /locus_tag="RHX88_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDV2228738.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            543..1931
                     /gene="cmtA"
                     /locus_tag="RHX88_000002"
     CDS             543..1931
                     /gene="cmtA"
                     /locus_tag="RHX88_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDV2228739.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1946..3223
                     /locus_tag="RHX88_000003"
     CDS             1946..3223
                     /locus_tag="RHX88_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV2228740.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3220..4185
                     /gene="yggF"
                     /locus_tag="RHX88_000004"
     CDS             3220..4185
                     /gene="yggF"
                     /locus_tag="RHX88_000004"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV2228741.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4207..4716
                     /gene="fumE"
                     /locus_tag="RHX88_000005"
     CDS             4207..4716
                     /gene="fumE"
                     /locus_tag="RHX88_000005"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDV2228742.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4713..5426
                     /locus_tag="RHX88_000006"
     CDS             4713..5426
                     /locus_tag="RHX88_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDV2228743.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQAVSSQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVVKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5398..6075)
                     /locus_tag="RHX88_000007"
     CDS             complement(5398..6075)
                     /locus_tag="RHX88_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV2228744.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6069..6746)
                     /locus_tag="RHX88_000008"
     CDS             complement(6069..6746)
                     /locus_tag="RHX88_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDV2228745.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6734..7441)
                     /locus_tag="RHX88_000009"
     CDS             complement(6734..7441)
                     /locus_tag="RHX88_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDV2228746.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7442..8020)
                     /locus_tag="RHX88_000010"
     CDS             complement(7442..8020)
                     /locus_tag="RHX88_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDV2228747.1"
                     /translation="MVRSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8043..8474)
                     /locus_tag="RHX88_000011"
     CDS             complement(8043..8474)
                     /locus_tag="RHX88_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV2228748.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8846..9865
                     /gene="epd"
                     /locus_tag="RHX88_000012"
     CDS             8846..9865
                     /gene="epd"
                     /locus_tag="RHX88_000012"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDV2228749.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9915..11078
                     /gene="pgk"
                     /locus_tag="RHX88_000013"
     CDS             9915..11078
                     /gene="pgk"
                     /locus_tag="RHX88_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDV2228750.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11293..12372
                     /gene="fbaA"
                     /locus_tag="RHX88_000014"
     CDS             11293..12372
                     /gene="fbaA"
                     /locus_tag="RHX88_000014"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDV2228751.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12730..13590
                     /gene="mscS"
                     /locus_tag="RHX88_000015"
     CDS             12730..13590
                     /gene="mscS"
                     /locus_tag="RHX88_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDV2228752.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13729..14364
                     /gene="argO"
                     /locus_tag="RHX88_000016"
     CDS             13729..14364
                     /gene="argO"
                     /locus_tag="RHX88_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDV2228753.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14457..15197
                     /locus_tag="RHX88_000017"
     CDS             14457..15197
                     /locus_tag="RHX88_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDV2228754.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15364..16260
                     /locus_tag="RHX88_000018"
     CDS             15364..16260
                     /locus_tag="RHX88_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2228755.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16257..17735)
                     /gene="scpC"
                     /locus_tag="RHX88_000019"
     CDS             complement(16257..17735)
                     /gene="scpC"
                     /locus_tag="RHX88_000019"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDV2228756.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAVADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17759..18544)
                     /gene="scpB"
                     /locus_tag="RHX88_000020"
     CDS             complement(17759..18544)
                     /gene="scpB"
                     /locus_tag="RHX88_000020"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDV2228757.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18555..19550)
                     /gene="meaB"
                     /locus_tag="RHX88_000021"
     CDS             complement(18555..19550)
                     /gene="meaB"
                     /locus_tag="RHX88_000021"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDV2228758.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPSHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPQVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLHHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19543..21687)
                     /gene="scpA"
                     /locus_tag="RHX88_000022"
     CDS             complement(19543..21687)
                     /gene="scpA"
                     /locus_tag="RHX88_000022"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDV2228759.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAQEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLESVRDVLNLISQHHD"
     gene            complement(21891..22784)
                     /gene="argP"
                     /locus_tag="RHX88_000023"
     CDS             complement(21891..22784)
                     /gene="argP"
                     /locus_tag="RHX88_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDV2228760.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22926..23111
                     /locus_tag="RHX88_000024"
     CDS             22926..23111
                     /locus_tag="RHX88_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2228761.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23167..23826
                     /gene="rpiA"
                     /locus_tag="RHX88_000025"
     CDS             23167..23826
                     /gene="rpiA"
                     /locus_tag="RHX88_000025"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDV2228762.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24082..25314
                     /gene="serA"
                     /locus_tag="RHX88_000026"
     CDS             24082..25314
                     /gene="serA"
                     /locus_tag="RHX88_000026"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV2228763.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25543..25602
                     /gene="ibsC"
                     /locus_tag="RHX88_000027"
     CDS             25543..25602
                     /gene="ibsC"
                     /locus_tag="RHX88_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDV2228764.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25935..25994
                     /locus_tag="RHX88_000028"
     CDS             25935..25994
                     /locus_tag="RHX88_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV2228765.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26095..26697)
                     /locus_tag="RHX88_000029"
     CDS             complement(26095..26697)
                     /locus_tag="RHX88_000029"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDV2228766.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26718..26901)
                     /gene="ssrS"
                     /locus_tag="RHX88_000030"
     ncRNA           complement(26718..26901)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RHX88_000030"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26943..27272)
                     /gene="zapA"
                     /locus_tag="RHX88_000031"
     CDS             complement(26943..27272)
                     /gene="zapA"
                     /locus_tag="RHX88_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV2228767.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27440..28018
                     /gene="ygfB"
                     /locus_tag="RHX88_000032"
     CDS             27440..28018
                     /gene="ygfB"
                     /locus_tag="RHX88_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDV2228768.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSGTSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28044..29369
                     /gene="pepP"
                     /locus_tag="RHX88_000033"
     CDS             28044..29369
                     /gene="pepP"
                     /locus_tag="RHX88_000033"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDV2228769.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29366..30544
                     /gene="ubiH"
                     /locus_tag="RHX88_000034"
                     /gene_synonym="visB"
     CDS             29366..30544
                     /gene="ubiH"
                     /locus_tag="RHX88_000034"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDV2228770.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREELLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30567..31769
                     /gene="ubiI"
                     /locus_tag="RHX88_000035"
                     /gene_synonym="visC"
     CDS             30567..31769
                     /gene="ubiI"
                     /locus_tag="RHX88_000035"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDV2228771.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWSKAQQASDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSSALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31843..31926)
                     /locus_tag="RHX88_000036"
     CDS             complement(31843..31926)
                     /locus_tag="RHX88_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2228772.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            32216..33310
                     /gene="gcvT"
                     /locus_tag="RHX88_000037"
     CDS             32216..33310
                     /gene="gcvT"
                     /locus_tag="RHX88_000037"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDV2228773.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33334..33723
                     /gene="gcvH"
                     /locus_tag="RHX88_000038"
     CDS             33334..33723
                     /gene="gcvH"
                     /locus_tag="RHX88_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDV2228774.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33842..36715
                     /gene="gcvP"
                     /locus_tag="RHX88_000039"
     CDS             33842..36715
                     /gene="gcvP"
                     /locus_tag="RHX88_000039"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDV2228775.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            36976..37719
                     /locus_tag="RHX88_000040"
     CDS             36976..37719
                     /locus_tag="RHX88_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2228776.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37776..39209)
                     /gene="bglA"
                     /locus_tag="RHX88_000041"
     CDS             complement(37776..39209)
                     /gene="bglA"
                     /locus_tag="RHX88_000041"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDV2228777.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            39254..39565
                     /locus_tag="RHX88_000042"
     CDS             39254..39565
                     /locus_tag="RHX88_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV2228778.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTITLETLTEKHAEQENMTLPELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            39729..40388
                     /locus_tag="RHX88_000043"
     CDS             39729..40388
                     /locus_tag="RHX88_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDV2228779.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40465..41445)
                     /gene="ygfZ"
                     /locus_tag="RHX88_000044"
     CDS             complement(40465..41445)
                     /gene="ygfZ"
                     /locus_tag="RHX88_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDV2228780.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41688..41954
                     /gene="sdhE"
                     /locus_tag="RHX88_000045"
     CDS             41688..41954
                     /gene="sdhE"
                     /locus_tag="RHX88_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDV2228781.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            41935..42342
                     /locus_tag="RHX88_000046"
     CDS             41935..42342
                     /locus_tag="RHX88_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDV2228782.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42382..42903)
                     /gene="fldB"
                     /locus_tag="RHX88_000047"
     CDS             complement(42382..42903)
                     /gene="fldB"
                     /locus_tag="RHX88_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDV2228783.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            43015..43911
                     /gene="xerD"
                     /locus_tag="RHX88_000048"
     CDS             43015..43911
                     /gene="xerD"
                     /locus_tag="RHX88_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDV2228784.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            43936..44646
                     /gene="dsbC"
                     /locus_tag="RHX88_000049"
     CDS             43936..44646
                     /gene="dsbC"
                     /locus_tag="RHX88_000049"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDV2228785.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            44652..46385
                     /gene="recJ"
                     /locus_tag="RHX88_000050"
     CDS             44652..46385
                     /gene="recJ"
                     /locus_tag="RHX88_000050"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDV2228786.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46476..47574
                     /gene="prfB"
                     /locus_tag="RHX88_000051"
     CDS             join(46476..46550,46552..47574)
                     /gene="prfB"
                     /locus_tag="RHX88_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDV2228787.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47584..49101
                     /gene="lysS"
                     /locus_tag="RHX88_000052"
     CDS             47584..49101
                     /gene="lysS"
                     /locus_tag="RHX88_000052"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV2228788.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(49144..49692)
                     /gene="idi"
                     /locus_tag="RHX88_000053"
     CDS             complement(49144..49692)
                     /gene="idi"
                     /locus_tag="RHX88_000053"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDV2228789.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            49747..49818
                     /locus_tag="RHX88_000054"
     CDS             49747..49818
                     /locus_tag="RHX88_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2228790.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(49815..49940)
                     /locus_tag="RHX88_000055"
     CDS             complement(49815..49940)
                     /locus_tag="RHX88_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2228791.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(49942..51390)
                     /gene="uacT"
                     /locus_tag="RHX88_000056"
     CDS             complement(49942..51390)
                     /gene="uacT"
                     /locus_tag="RHX88_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDV2228792.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            51826..53745
                     /gene="ygfT"
                     /locus_tag="RHX88_000057"
     CDS             51826..53745
                     /gene="ygfT"
                     /locus_tag="RHX88_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDV2228793.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     VAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNACEEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            53745..54233
                     /locus_tag="RHX88_000058"
     CDS             53745..54233
                     /locus_tag="RHX88_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV2228794.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(54269..55636)
                     /gene="ghxQ"
                     /locus_tag="RHX88_000059"
                     /gene_synonym="ygfQ"
     CDS             complement(54269..55636)
                     /gene="ghxQ"
                     /locus_tag="RHX88_000059"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDV2228795.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(55672..56988)
                     /gene="guaD"
                     /locus_tag="RHX88_000060"
     CDS             complement(55672..56988)
                     /gene="guaD"
                     /locus_tag="RHX88_000060"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDV2228796.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(57006..58406)
                     /gene="xanQ"
                     /locus_tag="RHX88_000061"
     CDS             complement(57006..58406)
                     /gene="xanQ"
                     /locus_tag="RHX88_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDV2228797.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(58571..61441)
                     /locus_tag="RHX88_000062"
     CDS             complement(58571..61441)
                     /locus_tag="RHX88_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDV2228798.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(61438..62217)
                     /locus_tag="RHX88_000063"
     CDS             complement(61438..62217)
                     /locus_tag="RHX88_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDV2228799.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(62268..63596)
                     /gene="ssnA"
                     /locus_tag="RHX88_000064"
     CDS             complement(62268..63596)
                     /gene="ssnA"
                     /locus_tag="RHX88_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDV2228800.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(63599..66697)
                     /gene="ygfK"
                     /locus_tag="RHX88_000065"
     CDS             complement(63599..66697)
                     /gene="ygfK"
                     /locus_tag="RHX88_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDV2228801.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQHNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ECFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNNKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFDNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(67019..67597)
                     /gene="mocA"
                     /locus_tag="RHX88_000066"
     CDS             complement(67019..67597)
                     /gene="mocA"
                     /locus_tag="RHX88_000066"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDV2228802.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            67700..68470
                     /gene="yqeC"
                     /locus_tag="RHX88_000067"
     CDS             67700..68470
                     /gene="yqeC"
                     /locus_tag="RHX88_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDV2228803.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            68518..70143
                     /locus_tag="RHX88_000068"
     CDS             68518..70143
                     /locus_tag="RHX88_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDV2228804.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFI
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(70184..71116)
                     /gene="arcC"
                     /locus_tag="RHX88_000069"
     CDS             complement(70184..71116)
                     /gene="arcC"
                     /locus_tag="RHX88_000069"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV2228805.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(71164..72549)
                     /gene="hyuA"
                     /locus_tag="RHX88_000070"
     CDS             complement(71164..72549)
                     /gene="hyuA"
                     /locus_tag="RHX88_000070"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDV2228806.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAVCGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRDYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNIREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(72602..73813)
                     /locus_tag="RHX88_000071"
     CDS             complement(72602..73813)
                     /locus_tag="RHX88_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDV2228807.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(73871..75067)
                     /gene="dpaL"
                     /locus_tag="RHX88_000072"
     CDS             complement(73871..75067)
                     /gene="dpaL"
                     /locus_tag="RHX88_000072"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDV2228808.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADETVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(75125..76312)
                     /gene="ygeW"
                     /locus_tag="RHX88_000073"
     CDS             complement(75125..76312)
                     /gene="ygeW"
                     /locus_tag="RHX88_000073"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDV2228809.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        18478 a        19124 c        20402 g        18606 t
ORIGIN      
        1 tttcattaaa ccgcacgagt attttatttt taccgccgga cggtacccta aaaataaaat
       61 aaggataatt tatgcggctt agtgattatt ttccagaatc atcaatctca gttatacatt
      121 cagcaaaaga ttggcaggaa gctatcgatt tctcgatggt atcattgctg gataaaaact
      181 atatcagcga gaattacatt caggcaatta aagattccac cattaacaat ggcccttatt
      241 atattctcgc accaggcgtg gcaatgcctc atgcgcgacc ggaatgtggg gcgcttaaaa
      301 ccgggatgtc tttgacatta cttgaacaag gtgtttattt tccggggaat gacgaaccaa
      361 ttaaattact catcggactc tctgctgccg atgccgattc gcacattggc gctattcagg
      421 cgttaagtga attactgtgc gaagaagaaa tactcgaaca actcttaaca gcatcatcag
      481 aaaaacaatt agcggacatt atcagccgcg gataattact ctctcctcta caggataata
      541 ttatggaaaa caagtctgct cgtgcaaagg tccaggcttt tgggggcttt ttgactgcaa
      601 tggtcatccc caatattggt gcttttattg cctggggttt tattactgcg ttatttattc
      661 ccaccggttg gctgcctaac gaacatttcg ccaaaattgt cggcccgatg attacctatt
      721 tattgcccgt gatgattggt tctacaggtg gtcatctggt cggcggtaaa cgcggggcgg
      781 tcatgggcgg aataggtact attggtgtga tcgttggcgc agagatcccg atgttccttg
      841 gctcaatgat catggggccg ctcggtgggt tggtcataaa atatgtcgat aaggcactgg
      901 aaaaacgcat acctgccggt tttgagatgg ttatcaataa cttctcatta ggtatcgcgg
      961 ggatgctcct ttgtctgctg ggttttgaag tgatcggccc ggcggtgtta attgccaata
     1021 ctttcgtcaa agagtgtatc gaggcgctgg tacatgcggg ttatctgcct ctgctgtcag
     1081 tcatcaatga accggcgaaa gtgcttttcc ttaataatgc gatcgatcag ggcgtctatt
     1141 atccgctggg aatgcaacag gcttcggtta acggtaaatc catcttcttt atggtggcct
     1201 ctaacccagg tccgggcctg gggctgctgc tggcgtttac cttgtttggt aaagggatga
     1261 gtaaacgttc tgcgcccggg gcgatgatta ttcacttcct cggtgggatc cacgaactgt
     1321 atttcccata tgtgctgatg aagccgctga ctattattgc catgattgcg ggcggtatgt
     1381 ctggtacctg gatgtttaac ttactggacg gtggtctggt ggctggtcca agtccggggt
     1441 ctatctttgc ttacctggca ctgacgccga aaggctcgtt cctggcgaca attgccggtg
     1501 ttacggtagg taccctggtg tcctttgcta ttacttcgct gatactgaag atggaaaaaa
     1561 cggtggaaac ggagagcgaa gatgagtttg ctcagtcagc caatgcggtt aaggcgatga
     1621 aacaagaggg tgcattctcg ttaagcaggg ttaagcgtat cgcctttgtt tgcgatgcgg
     1681 ggatgggctc cagtgcgatg ggcgcgacca ccttccgtaa acgcctggaa aaagcggggc
     1741 tggcaataga agtaaaacat tacgcgatag aaaacgtgcc tgcggatgcc gatatcgtcg
     1801 ttactcatgc cagtctggaa gggcgcgtga aacgtgttac ggataaaccg ctgatattga
     1861 ttaataacta tattggcgat ccaaaactcg acactttatt taatcaatta accgccgaac
     1921 ataaacactg attacagagg taaaaatgaa aaccaaagtt gctgctattt atggcaagcg
     1981 ggatgtccgt ctgcgcgaat ttgaactgcc agaaattacc gataatgaat tactggtgag
     2041 tgtaatttct gacagcgtct gtttatccac ctggaaagct gcgttactcg gtagtgaaca
     2101 taaacgcgta cccgacgatt tagaaaatca tccggtcatt accgggcatg aatgtgccgg
     2161 ggttattgtc gaagtgggta aaaatctcac tggcaaatat aaaaaaggcc agcgttttgt
     2221 attgcaacca gcgatggggt taccaagcgg atattcagcg ggctacagct acgaatattt
     2281 tggcggcaat gccacttata tgattattcc cgaaatcgcc attaatttgg gctgcgtatt
     2341 accgtatcac ggctcttatt ttgctgcggc gtcgctggca gagcctatgt gctgcattat
     2401 tggtgcttat catgccaatt atcacaccac gcaatatgtt tatgagcatc gcatgggcgt
     2461 caaacctggc ggcaatattg cactgctggc gtgtgcaggt ccgatgggca ttggcgctat
     2521 cgattacgcc attaacggcg gcatacaacc gtcgcgggtg gtggtggtcg atatcgacga
     2581 caaacgtctg gcgcaggtgc agaagctgct gccggtggat ctggcggcca gtaaaggcat
     2641 tgagctggtg tatgtgaata ccaaagggat gagcgatccg gtccagacgc tgcgggcgct
     2701 gacaggagat gtcgggttcg atgacatttt tgtttatgcg gcggtgcctg ctgtcgttga
     2761 gatggctgac gaattactgg cggaagatgg ctgtctgaac ttctttgccg ggccgacgga
     2821 taaaaacttc aaagtgccgt ttaatttcta caacgtccat tacaacagca cgcacgtagt
     2881 cggtacatcc ggcggttcaa cggacgacat gaaagaggcg attgcgttaa gcgccacggg
     2941 gcagttgcag ccgtccttta tggtcacgca tatcggtggg ctggatgcgg tgccagatac
     3001 cgtgctcaat ctgccggata tccctggcgg taaaaaactc atttataacg gcgtgaccat
     3061 gccgctcact gccattgccg attttgccga aaaaggcaaa accgatccgc tgtttaaaga
     3121 gttggcgcgg ctggttgagg aaacgcacgg catctggaat gaacaggccg agaaatatct
     3181 gctggcacaa tttggcgttg atatcgggga ggccgcgcaa tgatgtccct ggcgtggcca
     3241 ttatttcgcg ttacggaaca ggcagcgttg gctgcctggc cgcaaaccgg atgtggcgac
     3301 aaaaataaaa ttgatggcct ggcggtcacc gcgatgcgcc aggcattaaa cgacgtcgct
     3361 tttcgtggac gagtggttat cggcgaagga gaaattgacc atgcgccgat gctgtggatt
     3421 ggcgaagagg tgggcaaagg cgatgggcca gaagtcgata tcgcggttga cccgattgaa
     3481 ggcacgcgga tggtagcgat ggggcagagc aatgcgctgg cggtcatggc tttcgcccct
     3541 cgagatagcc tgttacatgc gcccgatatg tatatgaaaa agctggttgt taatcgactg
     3601 gctgccgggg cgattgatct gtctttgcct ctggcggaca acttgcgtaa cgtggcgcgg
     3661 gcgttaggta aaccgctgga caagctgcgc atggttacgc tggataaacc gcgcctgagt
     3721 gccgcgattg aagaagcaac acagctgggc gtgaaggttt tcgctctgcc ggatggcgat
     3781 gtcgccgcca gcgtgctgac ctgctggcag gataatcctt acgacgtgat gtacaccatc
     3841 ggcggtgcgc cagaaggcgt gatttctgcc tgtgccgtta aagcattagg tggggatatg
     3901 caggccgagc tgattgactt ttgtcaggct aaaggggatt acacggaaaa tcggcagatt
     3961 gcagagcagg agcgcaagcg ttgtaaggca atgggcgttg acgttaaccg tgtgtactcg
     4021 ctcgatgaac tggtgagggg gaacgatatc ctctttagcg ctacgggcgt gacgggcggc
     4081 gagttggtga acggtatcca acagacggcg aatggggtgc ggacgcagac attactgatc
     4141 ggcggcgcgg accaaacgtg taatataata gactccctgc attgatggtg atctatcgga
     4201 agaccaatgg cgacgctgac agaagatgat gtgcttgagc aactggatgc acaggacaat
     4261 ttattttcat ttatgaaaac tgcgcattct attttgctcc aggggatacg ccagtttctg
     4321 ccgtcgctgt ttgtcgataa cgatgaagag atcgtcgaat atgcagtgaa gccgttactc
     4381 gcccaaagcg gcccgcttga cgatattgat gttgcgctgc gtttgattta tgcgctgggg
     4441 aaaatggata aatggctgta tgccgatatc acgcactttt cccagtactg gcattacctg
     4501 aacgaacagg atgaaacgcc cggatttgcc gatgacatga cctgggattt tatcagcaac
     4561 gtcaatagca taacctgcaa tgcgacgctc tatgatgcgt taaaagcgat gaagttcgcc
     4621 gatttcgccg tctggtcaga ggcgcgtttt agcggaatgg tcaaaacggc gctgacgctg
     4681 gcagtaacga ccaccttaaa ggaattaacg ccgtgaaaat tgaattaacg gtgaatgggc
     4741 tgaacgtgca agcgcagtac catgatgaag agatagaacg cgtgcataaa ccgctactgc
     4801 gtatgctggc ggccttacag gcggtaagtt cgcagcgacg tacggtggtt tttctttgcg
     4861 ctccgccggg gacgggtaaa tctaccttaa ctaccttctg ggaatatctc gcgcaacaag
     4921 acccggaact gcccgctatt cagacgctcc cgatggatgg ttttcaccat tacaatagct
     4981 ggctggacgc gcatcaattg cgccccttca aaggcgcacc agagacattt gacgttgtga
     5041 aactggcgga aaatctgcgc caggtcgtgg aaggagattg tacgtggccg cagtacgatc
     5101 gacaaaagca tgatcctgtt gaagatgcgt tgcacgttac cgcaccactc gtcatcgtcg
     5161 aaggaaactg gttgttgctg gacgacgaga agtggtgcca gcttgcgcaa ttttgtgatt
     5221 tctcgatctt tatcaacgcg ccagctactg cgttgcggga acgactggta gggcgcaagc
     5281 tggcgggagg ggtatcgctg gcagacgcag aagcttttta tgaccgtacc gacgggccga
     5341 atgttcgccg ggtgctggaa gagagtctgc ctgcaaattt gaccttaatg atgaccgcta
     5401 cgggtgaata tcgtctggtg gattaacgtt cctgattaga ccatcttcca gtcgcaccac
     5461 acgagagaaa tgacgccggg taaatgcggc gtcatgactg attgctacga cgctcgtgcc
     5521 tcgctggccg cacttctcca gccagctttc aaacacgctc aaccaatttt cgtcaaaatc
     5581 ccgactcggc tcgtccagca gcaatagtgg cggagagagt gcttccaggc aggcgacggc
     5641 gaccatccgt cgctgcgcac tatgcagatc caacgggtgc gagttggcag tgtctgctaa
     5701 ctgacaacat tgcagggcag catgggtacg ttgagtgatt tcatccgccg ggcatttttg
     5761 cagtttcaaa ccaaatgcga tctcgtcagc gacggtgctg tgaaaaagtt ggttttccgc
     5821 ttcctgaaac agtacgccaa cctttgccgc gcgctggcgg tttttcaggt ttttcatcgc
     5881 ctgttgttgc agcataaccg taccggctgt gggcgtaaga agacctgcca ttactcgtaa
     5941 cagcgtcgat tttcccgcgc cattgtcgcc ggtcagcgcc aaccattcac cttgtttgag
     6001 ttgcagagaa atgtcgcaaa gacaatccgt tgccgcgcca ggccagcgat aagatatctt
     6061 atttaacgtt aacatagtgg ctggagcgct ccgttttgca attgccacgc ttgctggcag
     6121 tagttcagga aaggtgtgcg atgacgttca aaaagaataa tcagcgaacc acgttcaaac
     6181 gcccagtgtt gtaagcgctg caacagcatt tcgctggcct gcggagtcag gcggctaaag
     6241 gcttcatcaa gaattaagag ctttggctgc atcgcgatgg cgcaggcgat gaccacgcgc
     6301 tgtgtctctc caccggagag tgttgccggg tgacgatggc gcaacggctg gcactcggtc
     6361 agagtcaggg cggcatcaat ccgcgccata atctctgctt cagcaagaca cagattttct
     6421 ggcccaaagg caacttcttc ttccacgcta aaggtacagc cggagagctg caaatagggc
     6481 gattgctgga cgagctggat ggtggcggat tgttcattaa gcgggagatg cccaatgggc
     6541 gtaccgagga gcgtccccgt gcctgttatc tctcctggca gaaagtccgg ataccagccc
     6601 gccatcagct gtgccagcgt acttttgcca ctgccgttgt caccaaagat ggcgaccatc
     6661 cccggtttga catagtgaaa gtcataacag aacggaggat gcgttgagtg tgtcgggcaa
     6721 taacggaact gttctaacgt aaccatatcc acgctccgaa ttctgtcagc atcagcaaga
     6781 tcatggtgta acgcgctacg cgttgtaggg tgctatcggc tggtgcccac aacgttgttc
     6841 gtcggttgtg gatccgaaat gcccgcatat ccagcgccgc accacggaca gccagatcgt
     6901 taagcgcatt gtgtgttagc ggaataatta acgcgggcat cgcacgtaaa cgctgatacc
     6961 agccctcatc cagcggcacg ccgcgggcac gctgcgcttc atgaataatc gctaattgcc
     7021 gttttaactg ctcaacgacc aataacggcc cggcaaacag ataagcgacg cctggcggca
     7081 ggcgagaagc aaacagagcg cgaataaaac attgcacggg gacgtactgc atccacaact
     7141 gtgaggttga aacaatcgcc agaatccgca accagagcgt aatagcatgc gaccaccgtt
     7201 ctggcgagcg tggcgtgccg cttagccatt ctggtagcca gccaccgtgt accagccaca
     7261 gcccggctcc cagcgagaac atcagccaga cgacatattt tgcccgccgc cgcgtggcct
     7321 ttagcgcaat aaggcagaaa aaggtcgccg cgctatagat tggcaaaatg gtctgcgcgg
     7381 gcaggataag cgtggtgcag gccgccaatg cccacagtgt taatgaggta aatggatgca
     7441 tttagcgcac ggcagccatt gccggaaaat ggcgcgtggt gcgaatcggc agctggcgca
     7501 gtaaaagcca gacaatcacc gctgtaagaa ttttatcgac caggttcgcg cctatcaccg
     7561 taatcgccac cgactccacc aggttttgcc ccatcgagtg catccaggca acgaacaaat
     7621 cggctccgct accggtgaca ccgccgaaca gcgcagtacg cagcggtaca gcgacgaccg
     7681 ttaccgcaag cgtaataatc acgccgctga caacgacttt tggcagggtg cgaaaccaac
     7741 ccgctcgcgc caggcaaccg gcgaccaggc cgatgaccat cgcgacgggt gcaaatgcgg
     7801 cggcgatcgg gttggtcagc aatccccaga gtaaattggt cagtaagccg gtcagcattc
     7861 cgatcaccgg accaagcagt accgcgctaa taagtgtgcc gatggagtcg agaaatatag
     7921 gtaatttgac catgctggcc agttgcccgc cgatcatatt gatggcgata gatataacga
     7981 tcaacaccag tgcctgactg gaaaagtggc tacgcaccat aagaaaacct ctttgaaagt
     8041 agttatttaa cgggggaaat atgcaactcg tcgtaaccag aaagcgcgca aggttggcgg
     8101 ctaaacgcca gttcctcaag gctaccgatc accagttcaa gcgtggtccg cacggtacag
     8161 ccgggcatca tatgaccgcc aagcatcgtg ccgtgtggat cggaaacgca gagatgaaga
     8221 tgctcaccac tctgttccag cgtgccgttt agtgagatca cttcgaatgt accgttcagg
     8281 agcgtggtgc cttcttgccc ggcatagcgc aaggcgacgt cagtcaggct accggtacag
     8341 cctgcaatcc aggcggcatg aagttgatgc tgctgtgcaa aagcgtgtag ctgggaaaaa
     8401 acttcctgcc cggggaggag gcgcagtgcg taaaagcgtg cggaggaaga atatgggtag
     8461 ggcaatgtca tcattggagt actccgaaac tcggacgtat tgcggataat atcgacgctg
     8521 catcagtcat attctgatat aagtcagggt ttgtgacggc acaacgaaaa gattttgcta
     8581 cgcttattcg tgattctgca aagtggaata aattccacag gggcggggct gaaaacgtga
     8641 cgtaagtcag ataattgctt tctttgactg gacaatcatt ccttttattc cacgtttcgc
     8701 ttatcctagc tgaagcgttt cagtcgatta aatgttcgac aattaaccaa tcagtcgcag
     8761 tttgcgacag gtaaggtttc cccggacgat ttgctggatt actctgtctg gcaaatttgc
     8821 tcagggaaaa ccttgcagga gatctatgac cgtacgcgta gcgataaatg gcttcggtcg
     8881 catcgggcgt aatgtggttc gtgctttgta tgaatccgga cgccgggcgg aaattaccgt
     8941 ggtggcaatc aacgaactgg cggatgctgc gggcatggcg catttgttga aatatgacac
     9001 cagccatggc cgttttgcat gggaagtacg acaggaacgc gatcaacttt ttgttggtga
     9061 tgacgccatc cgcgtattgc atgaacgttc actgcaatcg ctcccctggc gtgaacttgg
     9121 cgttgatgta gtcctcgact gcaccggcgt atatggctcc cgcgagcatg gcgaagcgca
     9181 tattgccgcc ggggccaaaa aagtgctctt ttcacatcct ggcagtaacg atctcgacgc
     9241 gaccgttgtt tacggcgtca atcaggatca acttcgtgcg gaacaccgca tcgtttctaa
     9301 cgcttcctgt accacgaatt gcataattcc cgtcatcaaa ttgttagatg atgcgtacgg
     9361 tattgagtcc ggcacagtga ccacaattca ctccgccatg cacgatcaac aggttattga
     9421 tgcataccat cctgacctgc gtcgcacccg ggcagccagc cagtcgatca ttccggtcga
     9481 tactaaactg gccgccggta tcacacgatt ttttccgcaa tttaacgatc gctttgaagc
     9541 gattgcggta cgtgtgccaa ccataaatgt gacggcaatc gatttaagcg tgacggtgaa
     9601 gaaacctgta aaagccaatg aagtcaacct gttgctgcaa aaagcagcac aaggtgcatt
     9661 tcatggtata gttgactata cggaattgcc gttggtctct gtagatttta accacgatcc
     9721 gcacagtgcc attgtcgatg gcacccaaac ccgggtcagt ggcgcacacc tgatcaaaac
     9781 gttggtctgg tgcgataacg aatggggctt tgctaaccga atgctcgaca cgacgttagc
     9841 tatggctact gttgctttca ggtaagacgc aagcagcgtc tgcaaaactt ttagaatcaa
     9901 cgagaggatt caccatgtct gtaattaaga tgaccgatct ggatcttgct gggaaacgtg
     9961 tatttatccg tgcggatctg aacgtaccag taaaagacgg gaaagtaacc agcgacgcgc
    10021 gtatccgtgc ttctctgccg accatcgaac tggccctgaa acaaggcgca aaagtgatgg
    10081 taacttccca cctgggtcgt cctaccgaag gcgagtacaa cgaagaattc tctctgctgc
    10141 cggttgttaa ctacctgaaa gacaaactgt ctaacccggt tcgtctggtt aaagattacc
    10201 tcgacggcgt tgacgttgct gaaggtgaac tggttgttct ggaaaacgtt cgcttcaaca
    10261 aaggcgagaa gaaagacgac gaaaccctgt ccaaaaaata cgctgcactg tgtgacgtgt
    10321 tcgtaatgga cgcattcggt actgctcacc gcgcgcaggc ttctactcac ggtatcggta
    10381 aattcgctga cgttgcgtgc gcaggcccgc tgctggcagc tgaactggac gcgctgggta
    10441 aagcactgaa agaacctgct cgcccgatgg tggctatcgt tggtggttct aaagtatcta
    10501 ccaaactgac cgttctggac tccctgtcta aaatcgctga ccagctgatt gttggtggtg
    10561 gtatcgctaa cacctttatc gcggcacaag gccacgatgt gggtaaatcc ctgtacgaag
    10621 ctgacctggt tgacgaagct aaacgtctgc tgaccacctg caacatcccg gttccgtctg
    10681 atgttcgcgt agcaaccgag ttctctgaaa ctgcaccggc taccctgaaa tctgttaacg
    10741 atgtgaaagc tgacgagcag atcctggata tcggtgatgc ttccgctcag gaactggctg
    10801 aaatcctgaa gaatgcgaaa accattctgt ggaacggtcc ggttggcgtg ttcgaattcc
    10861 cgaacttccg caaaggtact gaaatcgtgg ctaacgctat cgcagacagc gaagcgttct
    10921 ccatcgctgg cggcggcgac actctggcag caatcgacct gttcggcatt gctgacaaaa
    10981 tctcctacat ctccactggc ggcggcgcat tcctcgaatt cgtggaaggt aaagtactgc
    11041 ctgcagtagc gatgctcgaa gagcgcgcta agaagtaaaa aatcacaggg cagggaaacc
    11101 tgcccttgtt tcagcgcgct tttagagcac gcattttttc atactctaaa taattcgagt
    11161 tgcaggaagg cgacaagaga gtgaatcccc aggagcttac ataagtaagt gactggggtg
    11221 agcgaacgca gacgcagcac atgcaacttg aagtatgacg agtataaggc ccgacgatac
    11281 aggacaagag acatgtctaa gatttttgat ttcgtaaaac ctggcgtaat cactggtgat
    11341 gacgtacaga aagttttcca ggtagcaaaa gaaaacaact tcgcactgcc agcagtaaac
    11401 tgcgtcggta ctgactccat caacgccgta ctggaaaccg ctgctaaagt taaagcgccg
    11461 gttatcgttc agttctccaa cggtggtgct tcctttatcg ctggtaaagg cgtgaaatct
    11521 gacgttccgc agggtgctgc tatcctgggc gcgatctctg gtgcgcatca cgttcaccag
    11581 atggctgaac attatggtgt tccggttatc ctgcacaccg accactgcgc gaagaaactg
    11641 ctgccgtgga tcgacggtct gctggacgcg ggtgaaaaac acttcgctgc taccggcaag
    11701 ccgctgttct cttctcacat gatcgacctg tctgaagaat ctctgcaaga gaacatcgaa
    11761 atctgctcta aatacctgga gcgtatgtcc aaaatcggca tgactctgga aatcgaactg
    11821 ggttgcaccg gtggtgaaga agacggcgtg gacaacagcc acatggacgc ttctgcactg
    11881 tacacccagc cggaagacgt tgattacgca tacaccgagc tgagcaaaat cagcccgcgt
    11941 ttcaccatcg cagcgtcctt cggtaacgta cacggtgttt acaagccggg taacgtggtt
    12001 ctgactccga ccatcctgcg tgattctcag gaatatgttt ctaagaaaca caacctgccg
    12061 cacaacagcc tgaacttcgt attccacggt ggttccggtt ctactgctca ggaaatcaaa
    12121 gactccgtaa gctacggcgt agtgaaaatg aacatcgata ccgataccca atgggcaacc
    12181 tgggaaggcg ttctgaacta ctacaaagcg aacgaagctt atctgcaggg tcagctgggt
    12241 aacccgaaag gcgaagatca gccgaacaag aaatactacg atccgcgcgt atggctgcgt
    12301 gccggtcaga cttcgatgat cgctcgtctg gaaaaagcat tccaggaact gaacgcgatc
    12361 gacgttctgt aagatattcc tttctgctta tctcaaggcc cgctctgcgg gtcttttttt
    12421 cgccaaaagc aaaattgcct gatgcgctac gcttatcagg cctacacgta tcctgcaata
    12481 tattgaattt gcaggttttt gtaggccgga taaggcgttc acgccgcatc cggcatgaca
    12541 aagcgcacat tgatatctgt ctgagccccg ctctgcgggg attttttcgc caaaagcaag
    12601 gtgattcaga tgagaaatct gtgatctatt tggcaaaatt atgctttatt gtttaccctt
    12661 gtcagactgc ccgtcataag gcggcggagt gtatttctcc attttgagtc agttgaaaag
    12721 gaatattgaa tggaagattt gaatgttgtc gatagcataa acggcgcggg aagctggctg
    12781 gtagctaacc aggcgctgct gctaagttat gcagtaaaca tcgtggcggc actcgcgatc
    12841 atcatcgttg gtttgattat cgcgcggatg atttccaacg cggtgaatcg cctgatgatc
    12901 tcccgtaaaa tcgatgccac tgttgctgat tttctctctg cattagtccg ttacggtatt
    12961 atcgccttta cgctaatcgc tgcactggga cgcgtgggtg tacaaaccgc gtccgtcatt
    13021 gctgtactcg gtgccgcagg cttagctgtt ggtctggctt tgcaggggtc actgtctaac
    13081 ctggccgctg gcgtgttact tgtcatgttc cgcccgttcc gtgccggaga atatgttgac
    13141 ctcggcggcg tagccggtac tgtgctgagt gtgcagattt tctccaccac catgcgtact
    13201 gcagacggta aaattatcgt tattccgaac ggtaaaatta ttgccggaaa tattattaac
    13261 ttctcccgcg agccagttcg ccgtaacgaa tttattattg gcgtggcgta tgattccgat
    13321 atcgatcagg ttaagcagat cctgaccaat attatccagt ctgaagatcg cattttgaaa
    13381 gatcgcgaaa tgactgtgcg cctgaacgaa cttggtgcat cgtcgattaa tttcgtggtc
    13441 cgcgtctgga gcaacagcgg cgatctgcaa aacgtgtact gggatgtgct ggagcgtatt
    13501 aaacgtgaat ttgatgccgc cggtatcagc ttcccgtacc cgcaaatgga tgtgaacttt
    13561 aagcgggtga aagaagacaa agctgcgtaa tcaacgctga ggccagataa tactctctgg
    13621 cctctctctt attagttatt ctgattgcca attaatatta tcaatttccg ctaataacaa
    13681 tcccgcgata tagtctctgc atcagatact taattcggaa tatccaacgt gttttcttat
    13741 tactttcaag gtcttgctct tggggcagct atgattctgc cgctcggccc acaaaatgct
    13801 tttgtgatga atcagggcat tcgtcgtcag tatcacatta tgattgcctt actttgcgct
    13861 atcagcgatt tggtcctgat ttgcgcgggg atttttggtg gtagcgcgtt attgatgcag
    13921 tcgccgtggt tgctggcgct ggtcacctgg ggcggcgtag tcttcttgct gtggtatggt
    13981 tttggcgctt ttaaaacagc gatgagtagt aatattgagt tagctagtgc cgaagtcctg
    14041 aagcaaggca gatggaaaat tatcgccacc atgttggcag tgacctggct gaatccgcat
    14101 gtttacctgg atacttttgt tgtactgggc agccttggcg ggcagcttga cgtcgaacca
    14161 aaacgttggt ttgcgctcgg gacaattagc gcctctttcc tgtggttctt tgggctggct
    14221 attctcgcag cctggctggc accgcgcctg cgcacggcaa aatcacagcg cattatcaat
    14281 ctggtagtgg gatgtgtcat gtggtttata gccttgcagc tggcgagaga cggtattgct
    14341 catgcacaag ccttgttcag ttaggcgcta tctgatggaa aaataaaaca gaggcgctaa
    14401 gcttgcctcc agaggtcctg aattttgggc aagtcaatta acacggagag actaacgtga
    14461 agttcaaagt tatcgccctg gcggcattaa tgggtattag cgggatggca gcgcaggcta
    14521 acgaattgcc ggatggaccg catattgtca cctccggtac ggcaagtgtg gatgcggtgc
    14581 cagacattgc cactcttgcg attgaagtta acgtggccgc gaaggatgcc gctacagcca
    14641 agaaacaggc agatgagcgc gtcgcacaat acatttcctt ccttgaactc aatcagatcg
    14701 cgaaaaaaga tatcagctca gcgaatttac gcacccagcc agattatgat tatcaggatg
    14761 gtaaaagtat cctgaaaggc taccgcgctg tgagaacggt ggaagtcacg ctccgtcagt
    14821 tagacaaact gaattccttg ctggatggcg cgctgaaggc gggtcttaac gaaattcgtt
    14881 ctgtgtcgct gggcgtggcg cagccggatg cctataaaga caaagcgcgt aaggcagcga
    14941 ttgataacgc gattcatcag gcgcaggaac tggcgaacgg ctttcatcgt aaactggggc
    15001 cggtatatag cgtgcgctac catgtttcca actatcagcc cagcccaatg gtgcggatga
    15061 tgaaagccga tgccgcgccg gtgtctgccc aggaaactta cgagcaggcc gctattcagt
    15121 ttgatgatca ggtcgatgtg gtcttccagt tagaacctgt ggatcaacaa cccgctaaaa
    15181 cacctgcagc acaataatac ttgtagcccg acgtattcgt cgggctaatg cctttacccg
    15241 atattgcctc ctttttctgt aattttctgt aaaaccatga cgtaatcacc tatctttgct
    15301 cgcggtttca tcaaggatag gctgactgaa acggttaagc ggacaaggat gcggggaggg
    15361 gggatggaca tttttatttc gaaaaagatg cgcaatttca ttttattagc gcaaaccaat
    15421 aatattgccc gggcggcaga aaaaatccat atgacggctt cgccatttgg taaaagcatt
    15481 gccgcgctgg aagaacaaat tggctatacg ctatttaccc gcaaagataa taatatcagc
    15541 ctcaataaag ccggtcagga actgtatcaa aaactgttcc cggtttatca acgactttct
    15601 gcgattgata atgaaatcca taactcaggg cgtcgttcac gagaaattgt gattggcata
    15661 gataatactt atccgacaat tattttcgat cagttgatta gtctcggaga taagtatgaa
    15721 ggcgtgaccg cccagccagt tgagttcagc gaaaacggtg ttatcgataa tctttttgac
    15781 cgtcagttag attttattat ctccccacag catgtatctg cacgagttca ggaactggaa
    15841 aacctgacta tcagtgaatt accaccgctg cgattgggct ttctcgtttc tcgccgttat
    15901 gaagagcgtc aagagcaaga gctgcttcag gagctgccct ggctacaaat gcgctttcaa
    15961 aaccgggcta acttcgaagc aatgattgat gcgaatatgc gaccttgtgg gatcaatccc
    16021 accattattt atcgtcccta cagttttatg gccaaaatca gcgccgtgga gcgcggacat
    16081 tttctgacgg tgatcccgca ttttgcctgg cgactggtga atccggcaac gctgaaatat
    16141 tttgatgcgc cgcacaggcc gatgtatatg caggaatatc tttattcaat cagaaatcat
    16201 cgttataccg ccacgatgct tcagcatatt gctgaagatc gtgacgggac aaaccattaa
    16261 cccagcatcg agccggttgc aattaaatta cggtgtaagt cgaagacgtg gctaagatcg
    16321 tggtgaatat gtccgccggg cgcattttcc agatagcgat gcagataatc ccgatacata
    16381 ggatgtgcac aattatcaat gatagtgcgg gcgcgttgaa gcggggaaag accgcgcaga
    16441 tcggcgatcc cttgttcggt gatgatcact ttgacgctgt gttcgctgtg atcaacatgg
    16501 ctgcacattg gcacgacggt tgagatcttg ccttctttag caatcgacgg agccataaag
    16561 atcgacagat acgcgttgcg ttcaaaatca ccgctgccgc cgatgccgtt catcagatcg
    16621 accccggcta cgtgcgttga gttggcatgc ccgtaaatat caaactccag gccgacgttc
    16681 agagcgatga cgcccagacg acggatgatt tccgggttat tggaaatctc ctgcggacgc
    16741 aacacaatgc ggctggcaaa gtaatccata ttgtcgtaaa tcttgcgcag ggaatcggcc
    16801 gagattgtca ggctggaggc gctggccccg ctgattttgc cggtttccag taaatgcacc
    16861 accgattcct gtagcacttc cgaatacatc atgaacggag gaatttccgg gttttccccc
    16921 agacgcgcca ttaccgcatt attgatattg cccacgccac tttgcagcgg cagaaattcc
    16981 ggcggaatac gcccatgcgc catttcctgc aataagaacg tgaccacgtt atcggcaatc
    17041 tgctggcaca tgggattttg cttatccagc atattaccgg cgtcgggcaa gttggtttcc
    17101 acgacggcga caatcttttt cggatcgatt tgcacatagc gggtaccgac gcgatccatt
    17161 gcatggaaga tcgacacgct attgcgccgc ggtggcgcgc caggaatcac aatatctgcc
    17221 agttctgcaa cgcgcggatc gtgatagtga ttaagttcaa tgatcacttt cttcgctcgc
    17281 agcagccagg tcggcgcatt accgatcccg ctggttaacc agactcgacc atccggtgcc
    17341 agtgccgatg cttcaatgac ggcaacatca atgtcgccga agaaaccgta attgaccatt
    17401 tgcgccactt cgctcaaatg caggtcaacg aaactcaccg cgccctgatt gatcttttta
    17461 cgtaaaccgg acgatgtttg atatggcgca cgccaggaaa cagcatcggc gtcagaaagt
    17521 acatcgtcag cgacggcgct gattgacgcc cccgtcagca ggcgaatttg atacggcttt
    17581 ttggcctcat gctgttcgtt agctctgcgg gcaatcgcgg tgggtagggc tttcggcgaa
    17641 cccgccgggg taaagccgct aaatgccacc atgtcgttat gctggataat ttctgccgct
    17701 tcatcggcgg tcatccttgt ccactgagtt tccattttta ctccttcgtt cgcagggatt
    17761 aatgaccaac gaaattaggt ttacgttttt cgaggaaagc gttcattcct tcctggtaat
    17821 cttcgctgtc ataaaccgcg cgtcgcatcc cctgaatacg ttcaaattca tcggagttca
    17881 tggtgtgcgc ttcgcccagt acacgcagct cttctttgat aacagcaatg gctaacggcg
    17941 ctttctcaga gatgtggtgc gccatttgta aggtgaaatc ttccagttct tccacttcca
    18001 caacatggtt gaggatgccg acagccaacg cgcgctgggc ggtgattggc gaagcggtaa
    18061 aaatcagttc tttgacaatg tggaagcccg cgtcgcgggt caggttgtga atgccgacca
    18121 ggttatacgg gacgccgagg tttacaggcg tcattgagaa ggttgaggta ctggcggcga
    18181 taatcagatc ggaactcatg atcatttcaa atgcgccacc ccaaacacta ccttccacca
    18241 tcgaaatgat cggtttcggg aatttttgga tcatgcgggt gatttgacgc aatggatcat
    18301 cataggagag cggatcgcga ccgccagacg gcagttcgtg aatatcgtgg cctgcggaga
    18361 agactttgga tccactcggc gcgcgcaaaa tgatacagcg aatttccggc cggttgagat
    18421 cgcttaatgc ctgcataaga tcatcaataa agactttact taaggcatta agtttgcggc
    18481 catagttaaa ctcaatgacc gccactttgt tgatagtgac aacgttaaca tactgataag
    18541 acataaaaat tcctttaatc aaaatattgc gtctggataa attcactgag ctgccgcagg
    18601 ccggtgcgcg gtgagagcgt attgtttttg accgctaaaa gcgtctggcg gtaatagcga
    18661 tcgaaatctt cattcgcgaa caggtgatgc agtacttctt cttcggtctg cttacgcagc
    18721 cattccaccg attgttgttg ccgcacttgt tgtaaacgac cactggcagt tagcgcggtt
    18781 ttgaagtcga tgatggcgtg ccagatctca tcgattccac gtttttccag tgcgctacaa
    18841 gtcagaacct gtggctgcca ttcgtcgtat ttacgtcgca gaatatgcag ggcactctcg
    18901 tacatatgcc gggcaatggc gacattggta tggttatcgc catcgtcttt gttgataacg
    18961 agcagatcag ccacttccat cagccctttt ttaatgccct gcagatcatc gccgccaccg
    19021 gcaatttgca acgagataaa acagtccacc atgcgggcga cttccgtttc cgactgcccg
    19081 acgccaaccg tttcgacaat cactacgtca taacccgccg cttcgcataa cagcattaat
    19141 tcccgcgctc gctgactggc accgcccaga tgaccggagg atggtaccgg gcgaataaac
    19201 gccgcttcgg cacgcgccag gtcattcatg cgggttttat ccccgagaat gctaccgcca
    19261 gtgaccgggc tgctgggatc gaccgcaata accgcgacct ttaatccctc acgaatcaac
    19321 aacatgccaa aggcttcaag aaaggtactt ttccccgcgc cgggtgtgcc ggtaacgccc
    19381 agtcgcaggg cgttaccgca gtacggcata atggcatcaa gcagttgcgt acttagcgcc
    19441 tgatgactcg ggtgacggct ttccaccagc gtcatggcct gggcgagtgt ggcacgctca
    19501 ccctgacgta agcggcgaat actttctgcc agcgtggctt cattaatcat gatgctggct
    19561 tatcagattc agtacgtcgc gcacactctc gagcataggt gtacctggac cataaatcgc
    19621 cgccacgccg cgctcttgca ggaaggcgta atcctgcggt ggaatgacgc caccggcgac
    19681 tacgcagata tcttcgcgtc cccatttttt cagcgcttcg accagttccg ggatcagcgt
    19741 tttatgaccg gcagccagtg aggatgcgcc cactacgtga acgtcgtttt ctacggccag
    19801 gcgggcgatc tcttcaggtg tagagaacat cgggcttaaa tctacgtcga aaccgagatc
    19861 ggaataggcg ctggcgatca ctttcgcgcc gcgatcgtgt ccatcctggc ccatcttagc
    19921 gatcagaatg cgcgggcgac gaccattgtc ggcaaggaac tgctccgttt gcgcaacaat
    19981 ggcatcgaac tcggaggccg atttctcaga ctgatgatag ctttgcgcaa tcacgccggt
    20041 aacacactgg cttggcacca gataacggtc gaaagcgact tccagcgcat cggaaatttc
    20101 acccagggtg gcgcgaacgc gagcggcatt aacagcggca gccagcaggt tttcgttatg
    20161 ctgtgcggcg tgagtcaggg cgttcaacgc ggcggttacg gcggcatcat cacgggtggc
    20221 gcgaatgcgt tccagcgaag caatttgctc gttacgcacc atcacgttgt cgatctcaag
    20281 tacatcggtt tcgtcttcgt gatccagttt gtacttgttg acaccaacga tgacacgctt
    20341 gccctggtcg atcagcgact gttcgcgcgc tgaggcctct tcgatcattc gttttggcag
    20401 acctgcttcg atcgctttcg ccatgccacc ggcttcgtcg atctgttgga taatagctct
    20461 ggcttgtttg acgatttgat cggtcagcga ctcaatgtaa taggatccgg ccagtggatc
    20521 gacggtgcgg cagagttctg attcttcctg gatgatgatc tgggtgttgc gggcaatgcg
    20581 tgctgagaaa tcggtaggca aaccaagcgc ttcgtcaaag gcgttggtat gcagtgactg
    20641 agtaccgccc agcgtcgcag ccagcgcttc aatggtggtg cggataacgt tgttatacgg
    20701 atcctgttca gtcaggctcc agcctgaggt ctggcagtgg gtacgcagcg ccagtgattt
    20761 cggatcctgt gcgccaaatc cactgaccgc ttcgctccat aaataacgtg ccgcacgcaa
    20821 catggcgacg ttcataaaca gatccatgcc gataccgaag aagaacgaca ggcgaggagc
    20881 gaagtcatca attttcagtc cggcagagat tgctgctttg atgtactcaa tcccatcagc
    20941 gagcgtaaat gctacctgct gcacgcagtt ggcacccgct tcacccatgt ggtaaccgct
    21001 gatactgatg gtattaaatc gcggcatgtt gccggaacac caggcgatga tgtcggcgat
    21061 aatgcgcatt gacggttttg gcgggtaaat ataggtgttg cggcagaggt actctttgag
    21121 gatatcgttc tgaatggtgc cggtcagttt atcaggtgta acaccttgct cttgcgcggc
    21181 gacgatataa aacgccagta ctggtagcac tgcgccattc atggtcatcg aaaccgacat
    21241 tttatccagc gggatctggt cgaacaggac tttcatatct tccacggtgt cgatagcgac
    21301 gcccgctttg ccgacgtcac ccgccacgcg tgggttatcg gagtcgtagc cacggtgggt
    21361 ggcaaggtca aacgcaacgg aaagaccttt ttgcccggcg gccaggttac ggcgataaaa
    21421 agcgttggac tcttttgctg ttgaaaaacc agcatactga cggatggtcc acggttgggc
    21481 ggtatacata gtggcacgcg ggccacgaac gtagggcggc aaaccaggaa gggtacctgt
    21541 cacctccaga ttatcgagat cggcttcggt atacagcggc ttgatggcga tcccttccgc
    21601 ggtttgctga accagcgagt cgacagtttt ctcccgacgg ctcaattcct tgttggcaag
    21661 ctgttgccac tcctgctcgt tagacattat ttgctccgta aaacgcaatc aaaatgaggg
    21721 tatttgcgct tacggtgagc tttttacgtg ggtttgtcgc cagcaaaaaa aatgcattca
    21781 gacgtcgttt tttgccgcct gagatttgtc tattttgtga tgtgattcaa ggaatacaaa
    21841 aagaagtgag gataacgcct gatatgtgct atcaggcatt atttgatgga ttaatcctga
    21901 cgaaggactt tgtgaccata atcgagcaac gcatcagtga ctttacgcat catgcggctt
    21961 tcaggagcaa agcggtgcca gtagagcatc cggcgttgaa atagccccgg cgttaagtca
    22021 atcagttcac cgctggccag ttctttctcg atttgcaggt gcgggatcat acagcaggtg
    22081 gtgccctggc gagcaagttg tacgaacgct tctgaagaat taacgatatg gcagggcacg
    22141 ctgcctggag gcagatcgaa gttttgctgc aaaaaggcct ggtgcatatc gtcaagatgg
    22201 tcaaacgcga ccactggcgc tttcagtaat gccgaacgcg ttacgccgtt agggaaatat
    22261 ttttcggcaa agggttttga gctgacgaac agatagtcga gcgcaccaag tttatcgaca
    22321 agacagctcg gcagcgcctg atgttgaata ctcaccgcgc cgaccacttc gccgcggcgc
    22381 agacgttcct gagtgcgggt ttcatcttct acctgcaagt tgaggcggat aggcgaatca
    22441 gccaacacag gagccagtgc aggaagcaac cacgtcgcca gactgtcggc gttgaccgcc
    22501 agtgaaagca gcagcggagt cgaaccggtt tgttcatcgc ccagccactc ttcttccagc
    22561 aactccacct ggcgcagcag tgccagcagt ttttgccctt gttccgtcgg gcgcggcggt
    22621 acggtacgca ccaacagcgg ctgcccgaac atattttcca gttgcttaat gcgctgtgag
    22681 acggctgatt gtgtaatgca cagcttttgt gccgcgcgct caaatcctcg ttcacgtatc
    22741 accgcatcca gtgcctgtaa tgttctgtag tccgggcgtt tcattgctgt tgtctgctcc
    22801 tggtaaatgc tttttcctgc actatgacac aattttgtgt caggttgcat ataccgaagc
    22861 cgtcggcggg taacatttgt attcgtggtt aaacgcgcag cttaaaagat cagggatttg
    22921 cgctaatgca tttcgctcag cgagttcgcg ccctggttgt gttgaatggc gtggcgttat
    22981 tgcctctgtt cgcaccgtgg agcggcatac ccagaccgtt gtatgcttta tttgcggggc
    23041 gaaaggggat gcccgccatt gcgcgatatt ttatggatga gttaaccacg cggcttgcca
    23101 acggggtctg aatcgctttt tttgtatata atgcgtgtga aatttcatac cacaggcgaa
    23161 acgatcatga cgcaggatga attgaaaaaa gcagtaggat gggcggcact tcagtatgtt
    23221 cagcccggca ccattgttgg tgtaggtaca ggttccaccg ccgcacactt tattgacgcg
    23281 ctcggtacaa tgaaaggcca gattgaaggg gccgtttcca gttcagatgc ttccactgaa
    23341 aaactgaaaa gcctcggcat tcacgttttt gatctcaacg aagtcgacag ccttggcatc
    23401 tacgttgatg gcgcagatga aatcaacggc cacatgcaaa tgatcaaagg cggcggcgcg
    23461 gcgctgaccc gtgaaaaaat cattgcttcg gttgcagaaa aatttatctg tattgcagac
    23521 gcttccaagc aggttgatat tctgggtaaa ttcccgctgc cagtagaagt tatcccgatg
    23581 gcacgtagtg cagtggcgcg tcagctggtg aaactgggcg gtcgtccgga ataccgtcag
    23641 ggcgtggtga ccgataatgg caacgtgatc ctcgacgtcc acggcatgga aatccttgac
    23701 ccgatagcga tggaaaacgc cataaatgcg attcctggcg tggtgactgt tggcttgttt
    23761 gctaaccgtg gcgcggacgt tgcgctgatt ggcacacctg acggtgtcaa aaccattgtg
    23821 aaatgatctg acgggggaac ctcccccgtt aaaaaaattc tcttcattaa atttggtgac
    23881 atgtgtcacg cttttaccag gcaattgtcg attgctctaa ataaatcctc taaaccagca
    23941 tattcatcca agaattacct ttgcgtgata tttcctcaac atcgcgacgc aaacgttcat
    24001 attgccgcaa tattattttt tgatatgttg aaaggcggat gcaaatccgc acacaacatt
    24061 tcaaaagaca ggattgggta aatggcaaag gtatcgctgg agaaagacaa gattaagttt
    24121 ctgctggtag aaggcgtgca ccaaaaggcg ctggaaagcc ttcgtgcagc tggttacacc
    24181 aacatcgaat ttcacaaagg cgcgctggat gatgaacaat taaaagaatc catccgcgat
    24241 gcccacttca tcggcctgcg atcccgtacc catctgactg aagacgtgat caacgccgca
    24301 gaaaaactgg tcgctattgg ctgtttctgt atcggaacaa accaggttga tctggatgcg
    24361 gcggcaaagc gcgggatccc ggtatttaac gcaccgttct caaatacgcg ctctgttgcg
    24421 gagctggtga ttggcgaact gctgctgcta ttgcgcggcg tgccggaagc caatgctaaa
    24481 gcgcaccgtg gcgtgtggaa caaactggcg gcgggttctt ttgaagcgcg cggcaaaaag
    24541 ctgggtatca tcggctacgg tcatattggt acgcaattgg gcattctggc tgaatcgctg
    24601 ggaatgtatg tttactttta tgatattgaa aacaaactgc cgctgggcaa cgccactcag
    24661 gtacagcatc tttctgacct gctgaatatg agcgatgtgg tgagtctgca tgtaccagag
    24721 aatccgtcca ccaaaaatat gatgggcgcg aaagagattt cgctaatgaa gcccggctcg
    24781 ctgctgatta atgcctcgcg cggtactgtg gtggatattc cggcgctgtg tgatgcgctg
    24841 gcgagcaaac atctggcggg ggcggcaatc gacgtattcc cgacggaacc ggcgaccaat
    24901 agcgatccat ttacctctcc actgtgtgaa ttcgacaacg tccttctgac gccacacatt
    24961 ggcggttcga ctcaggaagc gcaggagaat atcggcctgg aagttgcggg taaattgatc
    25021 aagtattctg acaatggctc aacgctctct gcggtgaact tcccggaagt ctcgctgcca
    25081 ctgcatggtg gtcgtcgtct gatgcacatc cacgaaaacc gtccgggcgt gctaactgcg
    25141 ctgaacaaaa tcttcgccga gcagggcgtc aacatcgccg cgcaatatct acaaacttcc
    25201 gcccagatgg gttatgtagt tattgatatt gaagctgacg aagacgttgc cgaaaaagcg
    25261 ctgcaggcaa tgaaagctat tccgggtacc attcgcgccc gtctgctgta ctaattcccc
    25321 ttctctgaaa attaacgggc aggtcactga cttgcccgtt tttttatccc ttctccacac
    25381 cgttgccgcg ttgcgcattc tactttgcga gtctgctcca gaaaataaat caaagtcaga
    25441 tcaatgcgtt gtgtttattt aatggggatt tacgatggcg ggacagcatg gggctgtaac
    25501 ggtaaagccc tcaccgaagc gagggcttaa aggagaaggg ttatgatgcg acttgtcatc
    25561 atactgattg tactgttact gataagtttc ggagcttatt aacagtcagt ctcaggggag
    25621 gagtcatcct cccttaccct tactcactaa attaggtcaa agaatcaacg atgtcaatca
    25681 gggcgatgcg gggtgcatcg ccctgaaccg aactttttga tagccacttc atcgcccacg
    25741 tttactgccg taatagatcc tttttcccaa aaattatctc tgttttgcat ggggtttttc
    25801 gagcacgctc gaagaaaaga tttaaaggta gatcaatgcg ttgtggttat ttaaagagag
    25861 cttacgatag ctgggtagca tggggctgta acggtaaagc cctcaccgaa gcgagggctt
    25921 gaaggagaag ggttatgatg cgacttgcca tcatactgat tgtactgtta ctcataagtt
    25981 tcccagctta ttaacagtca gtcacagggg aggagcaatc ctcccttacc cttactcact
    26041 aaattaggtt aaagaatcaa cgatgtcaat cagggcgatg caggtgtatc gcccttacca
    26101 ctcccagact ttcgacggtg taaccaccgc aggaagaggg atatcccact cttcaacggg
    26161 gagtttttcc accaactgac aatcatgcgc ataacccacc ggttgcgttt tatagtgctg
    26221 ccagttttgt aaggtccgat cataaaaacc accgcccatt cccaggcgct gaccgtactc
    26281 atcaaaggcg accagcggtg tgatcagcac gtctaatcgg gaaaggggta gcacgtcacg
    26341 cacatccagt tttggctcat ggatcttcaa cctgttcatc accagttcgc tttgcggatg
    26401 gtaattcagg aacagcaaat taccggcact aaagggatgc aaaactggaa gatatacgcg
    26461 cttaccggcg cgccagagtt gttctattag cggctgggtg tcgagttcac catcaaaaga
    26521 gaggaataca gcgaccgtat gcgccatcac caccggggga taagtcatca tccgggtagc
    26581 ggcttgttga cccatttcct gctgttgttc cggcgttaac gcacgacgac gttgccgaat
    26641 cattttgcgg atttcttgtc gggataatgt cagtgggagt tctggtagtt gcgtcatggc
    26701 tggtgccaga taagaaggga atctccgaga tgccgccgca ggctgtaacc cttgaaccct
    26761 tggttcaagg tgaatgtgtc gtcgcagttt taaggcttct cggacggacc gagcatgctc
    26821 accaaccgcg gagcgccaca ttcttgtggt atgaaatatc ggctcagggg actggcccgc
    26881 ttgcgaacat ctcagagaaa ttttgtcttc acggttactc taccacagta aaccgaaaag
    26941 tgtcattcaa agttttggtt agttttttcg gtgatgcgac cttgttcaag taacgcttgt
    27001 tctatggtct gctgcagcat ccgaatacgc tgttccatac ttgccgcgta gtcacgagtc
    27061 ttcgcttttt cttgcgctaa ctcatagctg atattcaatg cggcaatgaa gaccaactgt
    27121 tcagtatttg tgactctagt gcgttctttc agatcttgca accgttggtt cagatcgtcc
    27181 gctgcctgat tcaacgcatc cctttggtca ggcgggcagt tcacacgcag tgaacggcca
    27241 aaaatttgga tatcgacggg ttgtgcagac atgccacctt cctgctgatt gactgcgctg
    27301 ccttcgtctt tagacccatg gtctgcgaag gggcgacact atagctaccc tgatgagaag
    27361 agacaagccc ttttctggtc caccagggcc caaagtggta gcatatcatg aatattcctc
    27421 cctttgacga cgaatgctta tgtctataca gaacgaaatg cctggttaca acgaaatgaa
    27481 ccagtatctg aaccaacaag ggacgggtct gacccctgct gagatgcatg gtttaatcag
    27541 tgggatgata tgtggcggta acgatgacag ctcatggcta ccgctacttc acgacctgac
    27601 gaacgaaggc atggctttcg gtcatgagct ggcacaggca ctgcgtaaaa tgcactctgg
    27661 caccagcgat gccctgcagg atgacggctt cctttttcag ctttatctgc ctgatggcga
    27721 tgatgtcagc gttttcgatc gggctgatgc attggcaggt tgggtcaatc acttcctgct
    27781 tggtcttggc gttacgcaac cgaagctgga taaagtgacc ggcgaaaccg gtgaagctat
    27841 cgacgatctg cgtaacattg cgcaactggg ttacgacgaa gacgaagatc aggaagagct
    27901 tgaaatgtcg cttgaagaga tcatcgaata cgttcgtgtt gccgcgctgt tatgccacga
    27961 cacctttact catccgcaac cgaccgcgcc agaagtacaa aaaccgactc tacactaaaa
    28021 acaaaaaacg taaggagagt gttatgagtg agatatcccg gcaagagttt cagcgtcgcc
    28081 gtcaggccct ggtggagcaa atgcaacccg gcagcgccgc gctgattttt gctgcaccag
    28141 aagtaacacg tagcgccgac agcgaatacc cctatcgtca gaacagtgac ttctggtact
    28201 tcaccggctt taacgaaccg gaagcggtgc tggtgctgat taaaagcgat gacactcata
    28261 accacagcgt tctgtttaac cgcgttcgcg acctgacggc ggagatctgg tttggccgtc
    28321 gcttaggcca ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg gcattcagcg
    28381 aaatcaatca gcaactttat caactactta acggcctgga tgtggtttac catgcccagg
    28441 gcgaatatgc atatgctgat gaaatcgtga acagtgcgct ggaaaaactg cgtaaaggtt
    28501 cgcggcaaaa tctcaccgca ccggcaacga tgatcgactg gcgtcctgtt gttcatgaaa
    28561 tgcgcctgtt caaatcgcca gaagagattg ccgtactccg ccgcgcggga gaaatcaccg
    28621 ccctggcaca tacccgggcg atggaaaaat gccgtccggg aatgttcgag taccatctgg
    28681 aaggcgaaat tcaccacgaa tttaaccgcc acggtgcgcg ctatccttcc tataacacca
    28741 ttgtcggcag cggtgaaaac ggctgcattt tgcactacac cgaaaacgag tgtgaactgc
    28801 gtgacggtga cctggtgttg attgacgcgg gttgtgaata caaaggttac gctggcgata
    28861 ttacccgcac cttcccggtc aacggcaaat tcactcaggc ccagcgtgaa atctacgaca
    28921 ttgtgctgga gtctctcgaa accagcttgc gcctgtatcg tccgggaact tccatgcagg
    28981 aagtcaccgg tgaagtggtg cgcatcatgg ttagcggcct ggtaaaactc ggcattctga
    29041 aaggtgatgt tgatgaactg atcgctcaga acgcccatcg tcctttcttt atgcatggcc
    29101 ttagccactg gttagggcta gatgtccatg acgttggcgt ttatggtcag gatcgctcgc
    29161 gcattctgga accggggatg gtactgaccg tagagcctgg tctgtatatc gcgccggatg
    29221 cggatgtgcc agaacaatat cgcggtatcg gcattcgtat tgaagacgac attgtgatta
    29281 ccgaaaccgg caacgaaaac ctcaccgcca gcgtggtgaa aaagccggaa gaaatcgaag
    29341 cgttgatggc agctgcgaga aagcaatgag cgtaatcatc gtcggtggcg gcatggcggg
    29401 cgcgacgctg gcgctggcta tttcccggtt aagtcacggg gcgttgccgg tacatttgat
    29461 tgaagcgact gcgccagagt cacatgctca tccgggcttt gatggacgag cgatagcgct
    29521 ggcggcgggt acctgtcagc aactggcgcg catcggcgtt tggcaatctc tggcggattg
    29581 cgcaactgcc atcaccaccg tgcatgtcag cgatcgtggt cacgctggat ttgtcaccct
    29641 cgccgcagaa gattaccaac tggcggcgct gggacaggtt gtcgaattgc acaatgtcgg
    29701 gcaacggctg tttgcattgc tgcgtaaagc acctggcgta acgctgcatt gccctgatcg
    29761 cgtggctaac gttgcccgta ctcagagtca cgttgaagtg acgctggaga gtggcgagac
    29821 gctgacgggc cgcgtgctgg tagccgctga tggcacccat tcagcgttag ccaccgcgtg
    29881 cggcgttgac tggcagcagg agccttacga acaactggca gtgattgcca acgttgctac
    29941 ttctgttgcg cacgaaggcc gcgcttttga acgcttcacg caacatggcc cgctggcgat
    30001 gttgccgatg tctgacggac gctgttcgct ggtctggtgt catccactgg aacggcgcga
    30061 agagctgctg tcgtggagtg acgagaagtt ttgccgtgaa ctccagtcgg cctttggctg
    30121 gcgacttgga aaaattaccc acgccggtaa acgcagtgct tatccgctgg cgttaacccg
    30181 cgccgccaga gctattaccc atcgtaccgt gctggtgggc aatgcggcgc aaactctgca
    30241 cccgattgcc gggcaagggt ttaacctcgg tatgcgagat gtcatgagcc ttgcggaaac
    30301 cctgactcag gcgcatgagc gcggagaaga catcggtgat tacggcatat tgtgccgcta
    30361 tcagcagcgt cgacagagcg atcgcgaagc aaccattggc gtcacggaca gccttgtaca
    30421 tctttttgcc aaccgttggg caccgctggt tgtcgggcgc aacatcggac tgatgacgat
    30481 ggaattattc accccggcac gcgatgtgct ggcgcagcgc accctcggtt gggtggcgcg
    30541 ttgaaggcgg ttttaaggaa taaacaatgc aaagtgttga tgtagccatt gttggcggcg
    30601 gcatggtggg gctggcggtt gcctgcggct tacaggggag cggcttacgc gttgccgtac
    30661 tggagcagcg cgtaccggaa cctctggcgg cggatgcacc accacaactg cgcgtttcgg
    30721 ctatcaatgc cgccagcgaa aaattactca cccgtcttgg cgtctggcag gacattctct
    30781 ctcgtagggc tagttgttat cacggtatgg aagtgtggga caaagacagt tttggtcaca
    30841 tttcgtttga cgatcaaagc atgggctata gccatcttgg gcatatcgtt gaaaactcag
    30901 tgattcacta cgcgctgtgg agcaaagcgc agcaggcgtc agatatcacc ctgttggccc
    30961 ccgcagaatt acagcaggtc gcctggggag aaaatgaaac tttcctgacg ctgaaagatg
    31021 gcagcatgtt aacggcgcgt ctggtgattg gcgcggacgg cgctaattcc tggttgcgca
    31081 acaaagcaga tattccgctg actttctggg attatcagca tcacgcgctg gtagcgacca
    31141 ttcgcacgga agaaccgcat gatgcggtgg cgcggcaggt tttccatggc gaaggcattc
    31201 tggccttttt accgcttagc gatccgcatc tttgctcgat tgtctggtca ctgtcgccag
    31261 aggaagcgca gcggatgcag caggcaagtg aagacgaatt taatcgcgcg ttaaatatcg
    31321 cttttgataa tcgcctgggc ttatgcaagg ttgagagcgc gcgtcaggtg ttcccactga
    31381 cggggcgtta tgcgcgccag tttgccgcgc accgtctggc gctggtgggc gacgccgcgc
    31441 ataccattca cccgctggcg gggcaggggg taaatcttgg ctttatggat gctgcagagc
    31501 tgattgctga actgaaacgg ttgcatcgtc agggtaaaga catcgggcag tacatttatc
    31561 tgcgtcgcta tgagcgtagc cgcaagcaca gttcggcgct gatgctggct ggtatgcagg
    31621 gattccgcga tctgttttcc ggtaccaatc cggcgaaaaa actgctgcgt gatattggtt
    31681 tgaaactggc cgacacgctt cctggcgtta aaccacaact catccgccag gcaatgggat
    31741 taaacgattt gcctgaatgg ctgcgttaaa aatttctctt ctgttgttta tttgataccc
    31801 atcacacttt catctcccgg ttttttcgcc gggagatttt cctcatttga aataaactaa
    31861 tttcacctcc gttttcgcat tatattttct aatgccatga ttttttgatt tagtgttttt
    31921 tgacattttt ttagttctta atattgcctt attcaaatta actttctaat ggcatcatct
    31981 ttatatagaa actagtgtgt tttttggttt tttattctgt cgcgattttg gcgtttttta
    32041 accataagct aatgtgatga tcaattttac cttatggtta acagtctgtt tcggtggtaa
    32101 gttcaggcaa aagagaacga ttgcgttggg gaccgggagt ggctccgatg ctgggtttcg
    32161 tggtgataat ttcaccatga aaagttgtca gccccgctta ttcaatgagg acaagatggc
    32221 acaacagact cctttgtacg aacaacacac gctttgcggc gctcgcatgg tggatttcca
    32281 cggctggatg atgccgctgc attacggttc gcaaatcgac gaacatcatg cggtacgtac
    32341 cgatgccgga atgtttgatg tgtcacatat gaccatcgtc gatctccgcg gcagccgcac
    32401 ccgggagttt ctgcgttatc tgctggcgaa cgatgtggcg aagctcacca aaagcggcaa
    32461 agccctttac tcggggatgt tgaatgcctc tggcggtgtg atagatgacc tcatcgtcta
    32521 ctactttact gaagatttct tccgcctcgt tgttaactcc gccacccgcg aaaaagacct
    32581 ctcctggatt acccaacacg ctgaaccttt cggcatcgaa attaccgttc gtgatgacct
    32641 ttccatgatt gccgtgcaag ggccgaatgc gcaggcaaaa gctgccacac tgtttaatga
    32701 cgcccagcgt caggcggtgg aagggatgaa accgttcttt ggcgtgcagg cgggcgatct
    32761 gtttattgcc accactggtt ataccggtga agcgggctat gaaattgcgc tgcccaatga
    32821 aaaagcggcc gatttctggc gtgcgctggt ggaagcgggt gttaagccat gtggcttggg
    32881 cgcgcgtgac acgctgcgtc tggaagcggg catgaatctt tatggtcagg agatggacga
    32941 aactatttct cctttagccg ccaacatggg ctggactatc gcctgggaac cggcagatcg
    33001 tgactttatc ggtcgtgaag ccctggaagt gcagcgtgag catggtacag aaaaactggt
    33061 tggtctggtg atgaccgaaa aaggcgtgct gcgtaatgaa ctgccggtac gctttaccga
    33121 tgcgcagggc aaccagcatg aaggcattat caccagcggt actttctccc cgacgctggg
    33181 ttacagcatt gcgctggcgc gcgtgccgga aggtattggc gaaacggcga ttgtgcaaat
    33241 tcgcaaccgt gaaatgccgg ttaaagtgac aaaacctgtt tttgtgcgta acggcaaagc
    33301 cgtcgcgtga tttacttttt tggagattga ttgatgagca acgtaccagc agaactgaaa
    33361 tacagcaaag aacacgaatg gctgcgtaaa gaagccgacg gcacttacac cgttggtatt
    33421 accgaacatg ctcaggagct gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc
    33481 gcaacggtta gcgcgggcga tgactgcgcg gttgccgaat cagtaaaagc ggcgtcagac
    33541 atttatgcgc cagtaagcgg tgaaatcgtg gcggtaaacg acgcactgag cgattccccg
    33601 gaactggtga acagcgaacc gtatgcaggt ggctggatct ttaaaatcaa agccagcgat
    33661 gaaagcgaac tggaatcact gctggatgcg accgcatacg aagcattgtt agaagacgag
    33721 taacggcttt attcctcttc tgcgggagag gatcagggtg aggaaaattt atgcctcacc
    33781 ctcactctct tcgtaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct
    33841 catgacacag acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg
    33901 accggacgcc gcgcaacagc aagaaatgct gaatgccgtt ggtgcacaat cgttaaacgc
    33961 gctgaccggc cagattgtgc cgaaagatat tcagcttgcg accccgccgc aggttggcgc
    34021 accggcgacc gaatacgccg cactggcaga actcaaggct attgccagtc gcaataaacg
    34081 cttcacgtct tacatcggca tgggttacac cgccgtgcag ctaccgccgg ttatcctgcg
    34141 taacatgctg gaaaatccgg gctggtatac cgcgtacact ccgtatcaac ctgaagtctc
    34201 ccagggccgc cttgaagcac tgctcaactt ccagcaggta acgctggatt tgactggact
    34261 ggatatggcc tctgcttctc ttctggacga ggccaccgct gccgccgaag caatggcgat
    34321 ggcgaaacgc gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt ccgatgtgca
    34381 tccgcaaacg ctggatgtgg tccgtactcg tgccgaaacc tttggttttg aagtgattgt
    34441 cgatgacgcg caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt
    34501 aggcactacc ggtgaaattc acgactacac tgcgcttatt agcgaactga aatcacgcaa
    34561 aattgtggtc agcgttgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa
    34621 acagggcgcg gatattgttt ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg
    34681 tggcccacac gcggcattct ttgcggcgaa agatgaatac aaacgctcaa tgccgggccg
    34741 tattatcggt gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg cgatgcagac
    34801 tcgcgagcaa catatccgcc gtgagaaagc gaactccaac atttgtactt cccaggtact
    34861 gctggcaaac atcgccagcc tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat
    34921 cgctaaccgc attcaccgtt tgaccgatat cctggcggca ggcctgcaac aaaaaggtct
    34981 gaagctgcgc catgcgcact atttcgacac tctgtgtgtg gaagtggccg acaaggcggg
    35041 cgtactggcg cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgcggt
    35101 tgggatcacc cttgatgaaa ccaccacgcg tgaaaacgta atgcagcttt tcagcgtgct
    35161 gctgggcgat aaccacgggc tggagatcga cacgctggac aaagacgtgg ctcacgacag
    35221 ccgctctatt cagcctgcga tgctgcgcga cgacgaaatc ctcactcatc cggtgtttaa
    35281 tcgctaccac agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct
    35341 ggcgctgaat caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc
    35401 cgagatgatc ccaatcacct ggccggaatt tgccgaactg cacccgtttt gcccgccgga
    35461 acaggctgaa ggttatcagc agatgattgc gcagctggct gactggctgg tgaaactgac
    35521 cggttacgac gccgtttgta tgcagccgaa ctccggcgca cagggcgaat acgcgggcct
    35581 gctggcgatt cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat
    35641 cccggcttct gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt
    35701 ggttgtggcg tgtgataaaa acggcaacat cgatctgact gatctgcgcg cgaaagcgga
    35761 acaggcgggc gataacctct cctgtatcat ggtgacttat ccttctaccc acggcgtgta
    35821 tgaagaaacg atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct
    35881 tgatggcgcg aacatgaacg cccaggttgg catcacctcg ccgggcttta ttggcgcgga
    35941 cgtttcgcac ctcaacctgc ataaaacttt ctgcattccg cacggcggtg gtggtccggg
    36001 tatgggaccg atcggtgtga aagcgcatct ggcaccgttt gtaccgggtc atagcgtggt
    36061 gcaaatcgaa ggcatgttaa cccgtcaggg cgcagtttct gcggcaccgt tcggtagcgc
    36121 ctctatcctg ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa
    36181 agcaagccag gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc aggatgcctt
    36241 cccggtgctg tataccggtc gcgacggtcg cgtggcgcac gaatgtattc tcgatattcg
    36301 cccgctgaaa gaagaaaccg gcatcagcga gctggatatt gccaagcgcc tgatcgacta
    36361 cggtttccac gcgccaacga tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac
    36421 tgaatctgaa agcaaagtgg aactggatcg ctttatcgac gcgatgctgg ctatccgcgc
    36481 agaaattgac caggtgaaag ccggtgtctg gccgctggaa gataacccgc tggtgaacgc
    36541 gccgcacatt cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc gtgaagttgc
    36601 ggtattcccg gcaggcgtgg cagacaaata ctggccgacg gtgaaacgtc tggatgatgt
    36661 ttacggcgac cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaattcac
    36721 tgattcgact attttctaaa ggcgcttcag cgccttttta gtcagatgac aaagtacaaa
    36781 agtgctcaga cagtcccctc gcccctttgg ggagagggtg aggggaacag gccggcactg
    36841 gcgcgaatat ttaccctcac cccggccctc tccctgaaag ggcgaggggg aaaagcgtgc
    36901 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaatgaattt
    36961 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    37021 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcaacaaaac
    37081 ctccacgcgg cgcaggaagt gatgaactta ataacgcaag ccggtggcaa agcattcgtg
    37141 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    37201 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    37261 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    37321 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    37381 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    37441 tacgcggcat cgaaaggggc gattgatacg ttaaccaccg gactatcgct ggaagtcgcc
    37501 gcgcagggga tccgggttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    37561 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    37621 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    37681 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    37741 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    37801 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    37861 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtggtgaac
    37921 gatacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    37981 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    38041 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    38101 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    38161 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    38221 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    38281 agataatcgc aggtgccttc acgcagtaca tcgagatcgc cgtcttccat tttgatgtta
    38341 aatccgcgac gctcccactc gttcaacaca taggacgggt aatagccgcg tagctgcaca
    38401 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    38461 cagcagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    38521 atgcgacgcg cagctttcac cgccagggcg ctggcgacaa actggtgatg cagcacctga
    38581 tacatcgtct cttccgggtt ctcatgctcg gtataaacca cgccggagca gcagtaaccg
    38641 aacagcggtg cacgccagtt acgctggttg ttaatttcgt tgaaggtcat ccagtatttg
    38701 actttatgct tatagcgttc aaacaccact tccgcgaaac ggacaaagaa atcaaccact
    38761 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    38821 agggtgatca ccggttcgat gttgtatttc agcagttcat cgaacatatc atcgtagaac
    38881 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    38941 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    39001 tgaccataaa aatcaacggc ttcatggttt ggatagtatt tgcctggcaa gacttctttg
    39061 gtgatttcgc gcggcacgcc gtgtgcgcca ccggtcagaa cgtcacaaat gctcggccct
    39121 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caactgcgcc gccccataag
    39181 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatgaat caattcttac
    39241 aggaaatagt accatgcagc caaacgacat cacttttttt caacgtttcc aggatgacat
    39301 tctggctgga cgtaaaacca tcaccatccg cgacgaatct gaatcgcact ttaaaacggg
    39361 cgatgtgctg cgcgttgggc gctttgaaga tgatggctat ttttgcacca tcgaagtcac
    39421 cgcaacctca accataacgc tggagacgct gacggaaaaa catgcagagc aggaaaatat
    39481 gaccctgcct gaactgaaaa aggtcattgc cgacatctat cccggccaga cacagtttta
    39541 tgtgattgaa tttaaatgcc tttaaattga actcacgcct gttagttgaa atttcgcatt
    39601 taatttcatg atttttcgga gattagtcca aaatcagaat ttattttagc taacaggtgt
    39661 tcactggaac tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctccgg
    39721 agtaagttat ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg
    39781 ccaacagcgt cagtcacggc attgggctgg tgtttggtat cgttgggctg gtgttgctac
    39841 tggttcaggc ggtggatctc aatgccagcg ccacggcgat aaccagttat agcctatatg
    39901 gcggcagtat gatcctgctg ttcctcgctt cgacgctcta tcacgccatt ccccatcaac
    39961 gggcaaaaat gtggctgaag aaatttgacc attgcgccat ttacctgttg attgccggaa
    40021 cctatacacc gtttttgctg gtggggttag attctccgtt agcgcgcgga ttgatgattg
    40081 ttatctggag cctggcattg ctggggattc tgttcaaact gaccatcgcg caccgattta
    40141 aaattttatc tctggtgact tatctggcga tgggctggtt gtcgctggtg gtaatttatg
    40201 aaatggcagt taagctcgcg gcaggcagcg ttaccttact ggcggtaggc ggcgtggttt
    40261 attcgctcgg ggtgattttc tatgtctgca aacgcattcc gtacaatcat gccatctggc
    40321 acggctttgt actcggcggt agtgtgtgcc actttctggc gatctatttg tatattgggc
    40381 aggcgtaatt gctgtgcctt tgtctgcatg aaaagacaca tcagtgtcgc ttcaggcaat
    40441 agatgcccga agcgatatag aaatttactc ttcgagcgaa tacggcagcg gctcgatatg
    40501 cagtgtattt gcatcgtcgc gcacgcggaa gatgctgtcc ggttccatat cgttattcat
    40561 caccacctgc accacgacct gaccatcttc cagtttgacc gcagccagca ccgtaccggt
    40621 acgacgccag ttttcgccca ttttcagctc taagtcttca cctgcttctg gcagtcggct
    40681 ggcgctacct gccagtaacc agagcgcacg tttgttggca ccgcggaatt tggcccgcgc
    40741 caccatctct tgtccggtat aacagccttt cttaaagctg ataccgccca gcgcctggag
    40801 attggtcgcc tgtgggataa actgcccgct gttggcggca tcaatcaccg ggaaacccgc
    40861 ttcaatgttt aatgccagcc actgttggct attgttcagt tccgcttcac cgcgcagttt
    40921 atcggtcagc atattagcag tagcttcatc ggttacgatc aggaaacgtt ctgccgggtg
    40981 ttcaaaccat agcaaagtgg tcgcgccttc tttgactacc tgtttttctt tcgaaggcag
    41041 ttcgctaaag agatttgcca gcgcggcgcg cgcctgaaaa ccggcaacac caagcagcac
    41101 acgctcgtcg tctggcgcga tggtcacttt agagaatacc gcatattttt tcagttcagt
    41161 cagctgcggt tcacgcacgc tgcgccgttc aatccatgca aagccatcgc cgtcgcggaa
    41221 cagacgtaaa ttgctccaca ttttaccttt ggcgtcgcaa tgggcggcga gcaggtgctg
    41281 atcttctgcc atctggctga catctgctgt cacctgaccc tgcatatatt tttcgctgtc
    41341 cgcgccagta atggtggcaa gcgcccagtc atcaagcgtc atcagcgtca gcggtaaacg
    41401 ggcagaagcc gtaggctgac ggggaggaaa aggtgtaaaa gccataatag agtcctgagt
    41461 tgcttaacgg cgtattaatg actaatggta aaagagctat gaggcaatgc aagtgctttc
    41521 actacgcgat ctgtgccttc tgcgaggcga cttccaaaaa aatgaagcca ttgaattgtt
    41581 gtgaattaat ttcgcgatcg cgatggtgtt tactgatatt cccgtaaaag aaggcgaaaa
    41641 acacgttaca atagccgggt actggatttt cgtgagacag gaagaagatg gacattaaca
    41701 acaaagcccg cattcattgg gcatgccgcc gtggtatgcg cgaactcgat atttcaatca
    41761 tgccgttttt cgaacatgag tacgacagct taagcgatga cgaaaaacgc atctttattc
    41821 gtctgctgga atgtgacgat ccggacctgt ttaactggct gatgaatcac ggtaaaccgg
    41881 ccgatgcaga actggaaatg atggtccgac tcatccagac acggaaccgg gaacgtggtc
    41941 ctgtggcaat ctgatttgcg cgtctcctgg cgcgcacagt ggctttcctt gctgattcat
    42001 gggctggttg ccgctgttat tttactcatg ccctggccac tcagttacac cccgttatgg
    42061 atggtgttac tttcgctggt ggtgtttgat tgcgttcgca gccagcggcg tattaatgct
    42121 cgccaggggg aaattcgctt gttgatggac gggcgtttgc gttggcaagg gcaggagtgg
    42181 tgcatcgtca aagcaccgtg gatgattaag agcggcatga tgctgcgttt acgttctgat
    42241 ggcggtaaac ggcaacattt atggctggca gccgacagca tggacgaagc cgaatggcgg
    42301 gatttacggc ggattttgtt gcaacaagag acgcaaagat aaagatgacg gcaaagcata
    42361 tgcgctaagc cgtcagtgag atcaggcgta atgctctgcc atttcgttga ggatttgctc
    42421 gcaccagctc tgaatacgct cgtcgctaag gtcatactgg ttagtttcat ccagcgccag
    42481 acccacgaac agttgcccgt cagcaatcac cggtttcggg ctggtaaatt catatccttc
    42541 cgttggccag tagccgacga acttcacgcc tttggtcgag agtttgtcat gcagcatacc
    42601 gagcgcatcg aggaaccact cgccgtatcc cagttgatcg ccaagcccat acagcgcaac
    42661 aattttacct tcaaggttca ggtcgtcgag ctgatcccag acggcttccc agtcttcctg
    42721 gatttcacca aaatcccagg tcgggatacc cagaatgagc acatcgtact gctccattaa
    42781 tttcggggag tcgtccttga ggttatgtaa ggtcaccagt tctgggccga taatatcgcg
    42841 gattttttct gccgccattt cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt
    42901 cataacgtaa gcttctcgat tctgctgtgg cttttgcggc tagtgtatca gaatcgcttt
    42961 agtcacggca caataaggca taatggatca aatgagtgac cataaggggc gcaagtgaaa
    43021 caggatctgg cacgcatcga gcagtttctt gatgctctgt ggctggaaaa aaatctggct
    43081 gaaaatacgt tgaacgctta ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac
    43141 cgcgggttga cgctggcgac ggcgcaaagt gacgatttgc aggcattact ggcagaacgg
    43201 ctggagggcg ggtataaagc caccagctca gcgcgtttgc tgagtgcagt gcgccgattg
    43261 ttccagtatc tttatcgcga aaagtttcgt gaagacgatc ccagtgcgca tctcgcttca
    43321 ccgaaattgc cccagcgttt gccaaaagat ttaagcgaag cgcaggtcga acgtttatta
    43381 caggcaccat taattgatca gccactggag ctacgcgata aagccatgct tgaagtgttg
    43441 tatgctaccg ggctgcgtgt ctctgaactg gtcggactga caatgagtga tatcagcctg
    43501 cgtcagggcg tagtgcgggt cattggtaaa ggcaacaaag agcgcctggt accgttaggt
    43561 gaagaggcgg tttactggct ggaaacctat ctggaacatg ggcgtccgtg gctgctgaat
    43621 ggtgtgtcaa ttgacgtgtt gtttcccagc cagcgtgcgc agcagatgac gcgacagacc
    43681 ttctggcacc gcattaaaca ttatgctgtg ctggcgggta tcgacagcga aaagctgtca
    43741 ccgcatgtat tgcgtcacgc ttttgccacc catttattaa atcatggtgc ggatttacgc
    43801 gtggtgcaga tgctactggg ccacagcgac ctctccacca cgcaaattta tacgcatgtc
    43861 gctaccgagc gtctgcggca acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa
    43921 agaacgggaa gatttatgaa gaaaggtttt atgttgttta ctttgttagc ggcgttttca
    43981 ggttttgctc aggctgatga cgcggcaatt caacaaacgt tagccaaaat gggcatcaaa
    44041 agcagcgata ttcagcccgc gcccgtagct ggcatgaaga cagttctgac taacagcggc
    44101 gtgttgtaca tcaccgatga tggtaaacat atcattcagg ggccaatgta tgacgttagc
    44161 ggcacggctc cggtcaatgt caccaataag atgctgttaa agcagttgaa tgcgcttgaa
    44221 aaagagatga tcgtttataa agcgccgcag gaaaaacacg tcatcaccgt gtttactgat
    44281 attacctgtg gttactgcca caaactgcat gagcaaatgg cagactataa cgcgctgggg
    44341 atcaccgtgc gttatcttgc tttcccgcgc caggggctgg acagcgatgc agagaaagaa
    44401 atgaaagcta tctggtgtgc gaaagataaa aacaaagcgt ttgatgatgt gatggcaggt
    44461 aaaagcgtcg caccagccag ctgcgacgtg gatattgccg accattacgc acttggcgtc
    44521 cagcttggcg ttagcggtac tccggcagtt gtgctgagca atggcacact tgttccgggt
    44581 taccagccgc cgaaagagat gaaagaattc ctcgacgaac accaaaaaat gaccagcggt
    44641 aaataattcg cgtgaaacaa cagatacaac ttcgtcgccg tgaagtcgat gaaacggcag
    44701 acttgcccgc tgaattgcct cccttgctgc gccgtttata cgccagccgg ggcgtgcgca
    44761 gtgcgcaaga actggaacgc agtgttaaag gtatgttgcc ctggcagcaa ctgagcggcg
    44821 tcgaaaaggc cgttgagatc ctttacaacg cttttcgcga aggaacgcgg attattgtgg
    44881 tcggcgattt tgacgccgac ggcgcgacca gcacggctct aagcgtgctg gcgatgcgct
    44941 cgcttggttg cagcaatatc gactacctgg taccaaaccg tttcgaagac ggttacggct
    45001 taagcccgga agtggtcgat caggcccatg cccgtggcgc gcagttaatt gtcacggtgg
    45061 ataacggtat ttcctcccat gcgggggttg aacacgctcg ctcgttgggc attccggtta
    45121 ttgttaccga tcaccatttg ccaggcgaaa cattacccgc ggcggaagcg atcattaacc
    45181 ctaacttgcg cgactgtaat ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt
    45241 atctgatgct ggcgctgcgc acctttttgc gcgatcaggg ctggtttgat gagcgtggca
    45301 tcgcaattcc taacctggca gaactgctgg atctggtagc gctggggaca gtagcggacg
    45361 tcgtgccgct ggacgctaat aatcgcattc tgacctggca ggggatgagt cgcatccgtg
    45421 ccggaaagtg ccgtccgggg attaaagcgc tgctggaagt ggcaaatcgc gatgcgcaaa
    45481 aactcgccgc cagcgattta ggttttgcgc tggggccacg tctcaatgct gccgggcgac
    45541 tggacgatat gtccgtcggt gtggcgctgt tgctgtgcga caacatcggc gaagcgcgcg
    45601 tgctggcaaa tgaactcgat gcgctaaacc agacgcgaaa agagatcgaa caaggaatgc
    45661 aagttgaagc cctgaccctg tgcgagaaac tggagcgaag tcgcgacacg ctacccggcg
    45721 ggctggcaat gtaccacccc gaatggcatc agggcgttgt cggtattctg gcttcgcgca
    45781 tcaaagagcg ttttcaccgt ccggttatcg cctttgcgcc agcaggtgat ggtacgctga
    45841 aaggttcagg tcgctccatt caggggctgc atatgcgtga tgcactggag cgattagaca
    45901 cactctaccc tggcatgatg ctgaagtttg gcggtcatgc gatggcggcg ggtttgtcgc
    45961 tggaagagga taaattcgaa ctctttcaac aacggtttgg cgaactggtt acagagtggc
    46021 tggacccttc gctattgcaa ggcgaagtgg tgtcagacgg tccgttaagc ccggccgaaa
    46081 tgaccatgga agtggcgcag ttgctgcgcg atgctggccc gtgggggcag atgttcccgg
    46141 agccgctgtt tgacggtcat ttccgtctgc tgcaacagcg gctggtgggc gaacgtcatt
    46201 tgaaggtgat ggtcgaaccg gtcggcggcg gtccactgct ggatggtatt gcttttaatg
    46261 tcgataccgc cctctggccg gataacggcg tgcgtgaagt gcaactggct tataagctcg
    46321 atatcaacga gtttcgcggc aaccgcagcc tgcaaattat catcgacaat atctggccaa
    46381 tttagcgtca tcttctctat aaaaaagagc gtggattggg tacaatcccg ctcttatcac
    46441 cgcattttga ctagctcaat aaaagaaatc agaccatgtt tgaaattaat ccggtaaata
    46501 atcgcattca ggacctcacg gaacgctccg acgttcttag ggggtatctt tgactacgac
    46561 gccaagaaag agcgtctgga agaagtaaac gccgagctgg aacagccgga tgtctggaac
    46621 gaacccgaac gcgcacaggc gctgggtaaa gagcgttcct ccctcgaagc cgttgtcgac
    46681 accctcgacc aaatgaaaca ggggctggaa gatgtttctg gtctgctgga actggctgta
    46741 gaagctgacg acgaagaaac ctttaacgaa gccgttgctg aactcgacgc cctggaagaa
    46801 aaactggcgc agcttgagtt ccgccgtatg ttctctggcg aatatgacag cgccgactgc
    46861 tacctcgata ttcaggcggg gtctggcggt acggaagcac aggactgggc gagcatgctt
    46921 gagcgtatgt atctgcgctg ggctgaatcg cgtggtttca aaactgaaat catcgaagag
    46981 tcggaaggtg aagtggcggg tattaaatcc gtgacgatca aaatctccgg cgattacgct
    47041 tacggctggc tgcgtacaga aactggcgtt caccgcctgg tgcgtaaaag cccgtttgac
    47101 tccggcggtc gtcgccacac atcgttcagc tctgcgtttg tttacccgga agttgatgat
    47161 gatattgata tcgaaatcaa cccggcggat ctgcgcattg acgtttatcg cgcgtccggc
    47221 gcgggcggtc agcacgttaa ccgtaccgaa tctgcggtgc gtattaccca catcccaacc
    47281 gggatcgtga cccaatgcca gaacgaccgt tcccagcaca agaacaaaga ccaggccatg
    47341 aagcagatga aagcgaagct ttatgaactg gagatgcaga agaaaaatgc cgagaaacag
    47401 gcgatggaag ataacaaatc cgacatcggc tggggcagcc agattcgttc ttatgtcctt
    47461 gatgactccc gcattaaaga tctgcgtacc ggggtagaaa cccgcaacac gcaggccgtg
    47521 ctggacggca gcctggatca atttatcgaa gcaagtttga aagcagggtt atgaggaacc
    47581 aacatgtctg aacaacacgc acagggcgct gacgcggtag tcgatcttaa caatgaactg
    47641 aaaacgcgtc gtgagaagct ggcgaacctg cgcgagcagg ggattgcctt cccgaacgat
    47701 ttccgtcgcg atcatacctc tgaccaattg cacgcagaat tcgacggtaa agagaacgaa
    47761 gaactggaag cgctgaacat cgaagtcgcc gttgctggcc gcatgatgac ccgccgtatt
    47821 atgggtaaag cgtctttcgt taccctgcag gatgtgggtg gccgcattca gctgtacgtt
    47881 gcccgtgacg atctgccgga aggcgtttac aacgagcagt tcaaaaaatg ggacctcggt
    47941 gacatcctcg gcgcgaaagg taaactgttc aaaaccaaaa ccggcgaact gtctatccac
    48001 tgtaccgagc tgcgtctgct gaccaaagca ctgcgtccgc tgccggataa attccacggc
    48061 ttgcaggatc aggaagcgcg ctatcgtcag cgttatctgg atctcatctc taacgatgaa
    48121 tcccgtaaca cctttaaagt gcgctcgcag atcctctctg gtattcgcca gttcatggtg
    48181 aaccgcggct ttatggaagt tgaaacgccg atgatgcagg tgatccctgg cggtgccgct
    48241 gcgcgtccgt ttatcaccca ccataacgcg ctggatctcg acatgtacct gcgtatcgcg
    48301 ccggaactgt acctcaagcg tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac
    48361 cgtaacttcc gtaacgaagg tatctccgta cgtcataacc cagagttcac catgatggaa
    48421 ctctacatgg cttacgcaga ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt
    48481 actctggcac aggatattct cggtaagacg gaagtgacct atggcgacgt gacgctggac
    48541 ttcggtaaac cgttcgaaaa actgaccatg cgtgaagcga tcaagaaata tcgcccggaa
    48601 accgacatgg cggatctgga taacttcgac tctgcgaaag cgattgctga atctatcggt
    48661 atccacgttg agaagagctg gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg
    48721 gcagaagcac atctgattca gccgaccttc attactgaat atccggcaga agtttctccg
    48781 ctggcgcgtc gtaacgacat taacccggaa atcacagacc gctttgagtt cttcatcggt
    48841 ggtcgtgaaa tcggtaacgg ctttagcgag ctgaatgacg cggaagatca ggcgcaacgc
    48901 ttcctggatc aggttgccgc gaaagacgca ggtgacgacg aagcgatgtt ctacgatgaa
    48961 gattacgtta ccgcactgga acatggctta ccgccgacag caggtctggg gattggcatc
    49021 gaccgtatgg taatgctgtt caccaacagc cataccatcc gcgacgttat tctgttcccg
    49081 gcgatgcgtc cggtgaaata agcattacgt tatgctcaca accccggcaa atgtcggggt
    49141 tttttattta agctgggtaa atgcagataa tcgtattctg gcttcgcgat ttgtcgcctg
    49201 catcaccatc cacggactga acgcccacgg cgtggcatca ataccgtgta atacatcagc
    49261 taaatcacac cattgataat ccatcacttc atcatcgttg atctgtaacg cactggtggt
    49321 gcgtgcggca aataccggac acacttcatt ttccacaatg ccactcggat cggtggcgcg
    49381 gtagcgaaag tcaggataga tagattcagg aggcgtaatt tccacgccaa gctcataacg
    49441 gcaacggcgg atcaccgcgt cttcgctgct ttctcccagt tgtgggtgcc cacaaaccga
    49501 gttagtccac acgccaggcc acgctttttt gctcagtgcg cggcgggtaa ctaataattg
    49561 ccccttggca ttaaacagcc aactggagaa cgcgagatgt aagagggtgt ctgccgtgtg
    49621 tgcggcatac ttttccagcg taccagtggg aactccctgt gcattcaata aaatgacgtg
    49681 ttccgtttgc ataatttctc acatgtaatt ctgatcactc atcattatag ataattgaag
    49741 agtgaaatga ttaaccaagt gagcgtttat cgacaaccgc ccgttttgag cggatgccga
    49801 caggtaaaaa ccatttaaaa aagtagcatc atatatttga agcgactttc aatggccatt
    49861 gcaacacagt cagaaatgac aggaatagag agagtaaaaa gcaacagcag actgaatata
    49921 gcgcgcatta aaaaattcat attattctcc atgctcattt ttcttcagct tgcgcatcag
    49981 aagccacata cgtactgtcc tgacttttaa atctttatca gagacggatt ccttcaccag
    50041 gtcagcatga tgctggtagc cattaaagaa gacattcagc acaacggcgc taagggttgc
    50101 gagcataatg ccgctatgca gcaacggttg cagtacggcc ggtaacttag aaaagaaatc
    50161 gtgagagagc gtcggagtca tgccaacgcc gagactgatc gccacaatat agaggttgta
    50221 acggttggtg gtgtagttac agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc
    50281 gaacatcacc agcccagcac cgcccagcac aaattgcgga atggaggcga ccagcaccgc
    50341 catttttggc accatgccaa acaggattaa tataattccc gaagaaatac acacccagcg
    50401 gctatgaacg cgcgtcacgc taaccaggcc aacgttttgt gaaaatgacg tgtgggggaa
    50461 gctgttaaac gttccgccta tcattgtccc tacgccatcg acacgcagtc cgcgaataat
    50521 atcgtgtgaa gagagtttac gaccgactat ttcacccagt gccaggaaca tccccatcga
    50581 ctcgataaac acgatgatta acacggcagt catggtcaga atggaaacgg gatcgaaaat
    50641 cggcatacca aatgacatcg gcgtgacaat cgcaaaccat gaagcatcat gtagcccgga
    50701 taaattgact tcattcatca tccacgaaag taaaaagcca aatacaatcc ccagtaatac
    50761 ggcgacgttg gacataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac
    50821 agcaaaggag atacctaaat aaacgggatt accatattgc ggattccctt tacctccggc
    50881 ggcccagtca atacccacct gaatgatgct aagcccaata gaagtaataa ccacaccggt
    50941 aaccagtggc gggaataaag gcatcaagcg accgataagt ggcgctaata atgtggtgat
    51001 aaaacccgcg gcgatagtgg caccaaatat ccccagcagg ccgatatccg ggttcatccc
    51061 aatggctatc attggtgtta cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat
    51121 ccccataaag cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag
    51181 atccgagcta atgagcatcg caatagcttc ttttgagagg cccagtcggt caccaatcat
    51241 aagaggaaca gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat
    51301 cagctttcct ggtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga
    51361 tgagggaagt tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagagtaa
    51421 aaagcaaccg tttacgttaa tgaaactgtc taaacacatg ctcgtggagt gccagttttt
    51481 gcgaggaagg ggagttagag acaggaattg cggaggcaat cacataatcg tggttaaacc
    51541 acgaaaatga gaagagaacc tgacaatgaa attatctgta attttctcat attgatattt
    51601 cattaatcat tctggcatgt cgcactctcg catttaatcg tttttatctg gatagcgctc
    51661 ttttggtcgg caatgttgaa ataatgaaag cggcatagat tgaggggcaa taatttgaac
    51721 tcagtcgcaa aattataatt aaaacggtca tgctacctaa tatatgcccg tttattgcat
    51781 cgcgaaattt gagaagacat aactcattaa atgaaaggga tgcaaatgaa taagtttatc
    51841 gctgctgaag ctgcggaatg tataggctgt catgcttgtg aaattgcctg tgcggtggca
    51901 cacaatcaag aaaactggcc gctgagtcac agtgacttta gaccgcgtat ccacgttgta
    51961 gggaaaggcc aggctgcgaa tccggtggcc tgccatcact gcaacaatgc cccttgcgtt
    52021 acggcttgtc cggttaatgc tctgactttc cagtccgata gcgtacaact ggacgagcaa
    52081 aaatgtattg gttgtaaaag atgcgcaatc gcttgcccct ttggcgtcgt tgagatggtc
    52141 gatacgattg cgcagaaatg cgacctttgt aaccagcgca gttctggcac gcaagcctgt
    52201 attgaagtct gcccaacaca ggcgttacga ctgatggacg ataaagggtt acagcagata
    52261 aaggtggccc gccagcgcaa aacggcagca ggaaaagcgt catcagtcgc tcagccatct
    52321 cgcagtgcag cgttgctccc cgttaactcg cgtaaaggcg cagataaaat ttcagcgagt
    52381 gaacggaaaa cccactttgg cgaaatctat tgcgggctgg atccactgca agcgacttat
    52441 gagagtgacc gctgtgttta ttgtgccgaa aaagctaact gcaactggca ttgcccgctg
    52501 cataacgcta ttccggatta catccgtctg gtacaggaag gaaagattat tgaagcggca
    52561 gaactttgcc accagaccag ttccttacca gaaatctgcg gcagggtatg cccacaggac
    52621 cgtctttgtg aaggcgcatg tactttgaaa gatcactctg gcgcagtctc tatcggtaat
    52681 ctggaacgct acatcaccga taccgcgctg gcgatgggct ggcgtcccga tgtcagcaaa
    52741 gttgttcccc gtagcgaaaa agtggcggtg attggcgctg ggccagcagg gttagggtgt
    52801 gctgatattc tggcgcgcgc gggagttcag gttgatgtct ttgatcgcca tccagaaatt
    52861 ggcggtatgc tgacttttgg cattcctcct ttcaaactcg ataaaacggt attaagccag
    52921 cggcgagaga tattcaccgc aatgggaatc gactttcatc ttaactgtga aattggccgc
    52981 gatattacct ttagcgattt aacttctgaa tatgatgcag ttttcatcgg cgtggggact
    53041 tacgggatga tgcgagcaga tctgccgcat gaagatgcac ccggtgtcat tcaggctcta
    53101 ccgttcctga ctgcccatac ccgccagctc atgggattgc cggagtctga agagtatccg
    53161 ctgacggacg tggaaggtaa gcgagtcgtg gtattgggcg gtggcgatac gacaatggat
    53221 tgtttgcgga cttccattcg cctcaatgct gccagcgtga cctgcgcgta tcgtcgtgat
    53281 gaagtcagta tgccgggctc gcgcaaagag gtggttaatg cgtgcgagga aggtgtcgag
    53341 tttcagttca atgttcaacc gcaatatatc gcttgtgacg aagatgggcg tttaactgcg
    53401 gtgggcctga ttcgtaccgc catgggtgag ccggggccgg atggtcgccg tcgtcctcgt
    53461 ccggtagcgg gttcagagtt tgaattgccc gccgatgttc tgattatggc ctttggtttc
    53521 caggcgcatg ccatgccgtg gttgcagggc agcggaatta aactcgataa atggggcctg
    53581 attcaaaccg gtgacgtcgg gtatttacct acccagacgc atctgaaaaa agtctttgct
    53641 ggtggggatg cagttcatgg cgcggatctg gttgtcactg caatggccgc aggaaggcag
    53701 gcggcgcgcg atatgttaac tctgtttgat acgaaggcat cgtgatgaaa tcgttaatta
    53761 tcgttaatcc ggctgactgt attggctgcc gtacttgtga agtagcctgt gtggtcgctc
    53821 acccttcaga acaggagttg aatgccgatg tctttttacc ccggctgaag gtacagcgac
    53881 tggacagcat cagtgcgccg gtgatgtgcc atcagtgtga aaacgcccct tgtgttggcg
    53941 cttgccccgt gggggcgctg acgatgggcg agcaggtggt gcagaccaat tctgcccgtt
    54001 gtattggctg tcagagttgc gtcagtgcgt gcccgtttgg aatgatcacc attcagtcgt
    54061 tgccggggga tacccgacaa caaattgtga aatgcgatct ctgtgaacag cgggaagagg
    54121 ggccagcctg cgttgaatct tgcccaacac aggcgctgca gttgcttact gaaagagaac
    54181 tcaggcgagt ccgccagcag cgtattgttg ccagcggtga gaatcctctg tgaaagaaat
    54241 ctccgcgctg aaaggcgcgg agaatctatt agattgccca accacccgcg taaaatgcga
    54301 ccagtgcggc agtaatgatc accgtaccaa tattcagctt ttgccattcg cgtgcaaaga
    54361 cgcggcctac gaccagtgtc acaaagccca gcataatacc ggtaacgata ttacaagtca
    54421 gaacgatgaa cacggcacac accaggccag ccatagcgtc aataaaatca ttgaaatcca
    54481 gcttcgagac gttacttaac atcagcaaac ccacgtacat cagagcgggt gcagtggcgt
    54541 aaccagggat caaaaatgac agcggtgata agaacagaat cagcaggaat aacgccccca
    54601 ctacggttgc ggtcaaccct gttttacccc cggcggcggt tcctgccgct gattcgatat
    54661 aaaccgctgc gggcgctgcg cccaccagac cggagaatat tgaacttact gagtcactgg
    54721 tcagggcttt gccgccgttg atgatctggt tgtctttatc caacaaattc gcctgaccgg
    54781 cgacggcacg gatggtgcca gtggcgtcga acactgcggt catcaccaat gccagtacac
    54841 tcggaagtac ggttggctgg agtgcgccca taatatcgag gctgaaaatc agagacttac
    54901 catcttcgcc agtcagactt ggcatcgcca ccagaccgtg gtatttcacc gctgggtcaa
    54961 agattaagcc gataatcgaa attgcgataa tcaccaacaa gatcccgccg ggtacgcggc
    55021 acttctccag accgaagatg accgccagcc ccagcaagct catcatcacc gggaaggagg
    55081 taaacgcacc gagcgccact ggcaagcctt caatcgggtt tttgataacc atacccacac
    55141 cgttagcagc aatcagcagc agaaacaggc cgataccgat acctgtaccg tgagcgatac
    55201 ccatcggtaa attacgtaag atccaggtac gcacaccggt tacggaaata gcggtgaaga
    55261 tgacgcccat cagaaagacc gcgcccagtg cgacaggaac gctaatttgt tgcccgagta
    55321 ccagactgaa tgcggtaaat gccgtcaagg aaatcgcaca accaatcgcc attggcaaat
    55381 tggcccacaa ccccatcagc aacgagccga agcccgcgac cagacaggtg gcaacaaaca
    55441 cagctgcggg aggaaaacct gctttgccca gcattcccgg aacgacgata acggaataaa
    55501 ccatggccag aaaggtcgtt aagccagcca gtacttcctg acgaacggta ctgccacgag
    55561 cggtaatttt aaaataatta tccagcgcgc cctgtggctt tggtgcgtcc ggtgtttgta
    55621 ggatgtctcc agacataatg atgtcctcac gaggatgtta aagttttatt attagttgcg
    55681 ttcgtacacc agacgaccat caacgtaggt gcggtagatc gaacggtcat cgcccaacgt
    55741 catcatcacg aacaatttgt cgactaaaga aacagagttg tcatagcgca gctgctgtag
    55801 cggagtggcg gtgggttcca tcaccacgaa atcagcctct ttgccaggta aaaagttgcc
    55861 aatcaaatcg tcaaggccca gagatttcgc tccgccgagc gtggccaggt aaaacgcttc
    55921 atatgccgag aggcgatagc cttgtaattg caatactttg taggcttcgt tcagcgtttg
    55981 cagcatgttg aaagtggttc cggcaccgat atccgttccc atgcccactt taactttctt
    56041 ctgccatgct tttttcaagt tgaataagcc gctgccgagg taaaggttgg aggtcggaca
    56101 gaaagcaatg ctggatttgg tttcgctgag acgatcccac tctttttctt cgagatggac
    56161 gcagtgagca aagacacagt ttttaccggt caggccgtac tgatggtaaa catccagata
    56221 accatcatgg tcaggataaa gcgatttcac ccaggcaatt tcatctttgt tttcacagag
    56281 atgggtatgt acccacgtat ccggatattc ttctttcagg cgttgcgcca tcgccatctg
    56341 ttcaggagat gaggtcgggg cgaagcgtgg cgtaatcgca tatagcagac gaccattttt
    56401 gtgccagcgt tcaatcagtt ctttgctttg gtgatagctg ctttcggcag tgtcgagcag
    56461 ataatccggt gcgttgcggt ccatcatcac cttaccggca atcatacgca tattgatatg
    56521 actggcggct tcaaacagcg catcaacaga ttgcggatga acagtgccaa acaccagcgc
    56581 cgtggtggtt ccgttacgta aaagctgctt gatgaagaat gccgacattt cgcgggcgta
    56641 ctctaaatcc tcataacgac gttcagtagg gaaggtgtgt ttattcaacc actccagcaa
    56701 ttgttcacca taggccccca ccatttcact ttgcggataa tggatatgtg tatcgacaaa
    56761 gcccggtact atcagtttgc cgcgatagtc gcgcacgcga atagtgtcag gaatttgatg
    56821 ctttccgtct tcccattcgc caaaccattc cacttttccc tgtttaatga gtaataaacc
    56881 atcctcaata aaccgcagcg cagaggcaat ctcttccggg ttatcgaccg tacgggtgac
    56941 atcaataaaa ctgcctcgta ccgctttcaa cgtgtgttct cctgacatca ttgactcctt
    57001 tactgttaat ccatctcttc cgctgaggta ataccaggca gaacgttttc ctgtcggtag
    57061 ccaccaggga ggataatatt taataaaatc gcagttaacc cgccagcaca aatagggttt
    57121 tcaactagta cataaataga ggctggcaat attttaaaaa tttcgggatc gtaggagacg
    57181 ccaagtccta aacctaaaga agtggcgaca ataagtgttt ctcggcgctt taaaccgttg
    57241 gtgatgatta tgcgaatccc agcgatggca atcatggaaa acatcaacgt cattgcgcct
    57301 cccagaactg ccgagggaat ggtcgtgaag aagccgccaa tcatcggaaa caaaccgagg
    57361 ataaccagca ttaccgcgat ggttcgcccg acataacgtg aagcgacgcc agtcatctga
    57421 ataaccccat tattttgcgc aaacgtggtt aagggtaatg acccgacagc ggaggcgata
    57481 acagaaacca gaccgtctgc cagcacgccg cctttcagtc gggactgata ctcttctccc
    57541 tgaatgggtc ggcgggaaac cattgccgtg gcggtgatat cgccgactgc ttccagcacg
    57601 ctaagcagat aaatcgtgcc aaccaccagg aactgatgga agctaaaact aaagccgtat
    57661 ttgaacggat gcgggatggt gattaacggc aaattgcgca tactgctgaa atccaccatg
    57721 cccaggcata acgatgcgat atagccgaca catagcccaa tggcgatccc tcccatgcgt
    57781 agcaacggac tgcgacagca gttaaagccg atcaccacaa ttaaaaccaa taaaccaacg
    57841 ccgagatgtt cgtaattgcc gaacgtaccg ctgcttttgg ctgcaaatcc tccaccaaaa
    57901 tcgataatgc cgactttaat caggcttaag ccgatcatca gtaccacaat accgctgacg
    57961 gtaggcgtaa taacccgacg taaatagggc aagataaatg aagatccgac aaccagaaat
    58021 gcgccaacga aggagacgcc gagaagcgac gacatgatta actcttcgtg aaaaccgtcg
    58081 cttttcatgc tgctgcccag cgcaatcatg accgtaacaa atgaaaaatt gactgactga
    58141 attgagagta ggccagaacc gacgatgccg tagcggttta cttgtaacca ggtaccaata
    58201 ccagaggcga tcatcgccat agaaacaaga taggcagttg tttcagcgga aagctgtaag
    58261 gccgccccca cgattaacgc gggggttacc atcggaacga aaattgccaa cagatgggta
    58321 atggcaccaa cgagagcctg atgaaaggga gggcgatcct ccagttcaaa tataaggtca
    58381 gaacctgcat tgtttatatc agacatccct tcactccttt taaagtaaat ttgatcatct
    58441 gaattagtaa aataaattcc cacgaatttg gggcgcagga cgccttgaga acggcttatc
    58501 tcattttcgc agtcactatt tagtcatcgt ccaaacagag aaacaggccg ccgaagcggc
    58561 catttattat ttatattttt tccagcgcag tgagtatttt ctccggtgtg aaatgccatt
    58621 cgcgtaacca gatgccgcag gcatcgtgaa ttgcggtagc aatcgccgga gctgcgccat
    58681 ttacaccaat ttccgagatc gatttcgccc cgaacgggcc gactttatcg tcgctcggca
    58741 ccaacacagc gcggaaatca cgcggaatgt caccaatttt cggtgcgccg taactgcgta
    58801 aatcacgcgt taacgggtga ccttcggcgt tataaatgat ctcttcgctc atactgtggc
    58861 cgatagcacg cagggtggca ccgtagattt gtcccaacgc taactctgga ttgaccggtg
    58921 taccgcagtc cagcaaggcg tagaatttat ccaggcggat ttcacccgta cgcgtgttga
    58981 cggcaacttc agcgaagttt gcgccatacg ggaaagcgaa atcaggcgtg atataactgc
    59041 cagttcccac cagtgaacca aagccggtgc cggtttcgcc tttatgggca atatccccga
    59101 aactaacttc gcctttcttg ccgcgcacga cgcccggcgt tgctagttga acatctgcca
    59161 ctggctcacc caacatttgc gcgccgtgga acagaatttt ctcccgcaga ttttccgctg
    59221 ccaaacgcgc cgcgttaccc gagaagcaag taccggacga ggcatatgcg cctttatcaa
    59281 acaacgcatg atcggtatca ccggagataa catgcacgtc ctgcggtggg cagtgcagca
    59341 cttctgctgc cagtttcgtc actacagtat ccagaccagt accaatatcc gcaccgccag
    59401 aatgaacgat aaaggtgccg tccgattcca gtttgatcat gcagttagcc tgatcgatat
    59461 ccgggatacc cgatttctgc atgataatgg cgacgccgcg accgatgtgc cagtcaccat
    59521 tttgcggttt cggtgaagac cattggatca tctcgcgacc ctgacgcagg atctcttcca
    59581 gtgcgcagct ggcggcggaa ggaacggagg tcggcgcttt accttcaccg attgcaccga
    59641 gaattttcag ctcttgccct tcgtgtaccc ggttacgttc gataatttcc agttggtcga
    59701 tctgtaactg ttcagccagt tccgctaatg ccatggtgat agcgaagtta cctttcggtg
    59761 cgccataacc ctgataagca ccatttgggc aaatgttgct gtagtaggtg gtgacctgga
    59821 aatcgacgtt atcgcacgga tataacggca gcgacagcgc cggtccgtta cacggtacgg
    59881 tgagtgagtg gttgccgtaa gggccagtgt tggcgcggaa atccatcttc actgccgtca
    59941 ggcgaccatc ttttttcgct cccagtttga cggtgacttt cgcgacgtga cgagaggtgt
    60001 tagcaataaa ctcttcttca cgggtgtagc ggaacagtac cggacgcccg gtcacgcagg
    60061 ttgcccatgc gcacacttct tccagcagga tgtcctgttt ggagccaaaa ccgccgccaa
    60121 ctcgctcttt aatgacatga actttatgct gtttcatgcc tacgaggcgc gcgacctggc
    60181 ggcgtaagtg ccatggtacc tgggtggagg cgtggataac cagacgatcg ccgtccatcc
    60241 gggtaaagca gatatgtgtt tcagtcgggc actgctgtgc ctgcgttgag ttataggttc
    60301 gctcaatgat cacatcggca tcggcaaagc ctttgtccat atcgccaata tgaccatgaa
    60361 tactggcggc gatatttttg cgagggcgag aaccgatcgg gaagttgatg atcatatgct
    60421 cgccgcgctg ggctgcatgg ctgttatcgt cttccagagt atctggcgca ccagcaacat
    60481 acaccaccgg ttcatcgtgc acgacaggcg catcttccgc cattgcttcg tcgatcgaca
    60541 ttaccggctt aagcacttca tattcaacgt cgatgagctt caatgcttcg agcgcaattt
    60601 cttcactttc tgcgacgacc gcagcaacac gatcgccgac gtgacgcatt ttcttgccga
    60661 acatacggcg gtcaagcggt gacggttccg gtgcgctctg accacccggg gtatagtaga
    60721 tatccgggca attcaggtga gtaataacgt gaacgacgcc cggtaaggct tcagctttgc
    60781 tgacatccag atgagtaatc agtgcgtgag cgtgtgggct acgtaacatt ttaatgacgc
    60841 aggcgtcagc cgttacgcgg tcttcaacat agcagggttt tgcctgcacc attttcgcgg
    60901 catcagtttt aggataatgc ttgccaatga cttcaaggtc gtcacggaaa gtcggagcga
    60961 tatcaatggt ggcctgcgga ttatttttac gtgcaaccgc cagttcaatg acctgatagt
    61021 attgttgcca gcctgcatcg cggctgaaca aaccagaaag cgcgtcgtcg atctcttcgc
    61081 gggtaggtgc ggcgatgcga tcgagaagat cggtgataat cagagctgca gctggatcgt
    61141 tataaccaga ctgcaccacg ccaacatcaa ccattgcctg ttgaaccaga cttaactcgt
    61201 tccatttgcc caaagattct gcggtacgaa tatctgcctt ctctaactgt gcggcaataa
    61261 gcaaggacgc gttaacgata ttaccgttaa agattattgc gtcagaaccg gcaaacccga
    61321 agccatcatc actgttgcgt acagagtgca ttcccatgtt aaacaacagc ttttgcacgt
    61381 tttcgcctgg attaacggtt agctcctgag gcgcgccatt taaagtaaag tggatgatca
    61441 tacggcttcc tccccagcct gttggcagtc ggcatacagg tcggctacca gaactcccgt
    61501 gatatagcgt ttataggcca cgctgccccg caaatcttcc tgcgggaaaa tggcgttggc
    61561 gacagcctgt tcaagtgcat tgccttccag attttgtttt tcgacatcat gcagacgcag
    61621 tgctttactg gcgacgccat ccagcgcaat tcgcataccg tcgtggtctg tcattgcaac
    61681 ggctgccgtc acgacggtta aacctgcctg agaacggcta attttgcggg tcgcacaggt
    61741 gcgatacgga tctttaatga taatttcggt tagcaggcga tcgcatgggc aggccaggta
    61801 gtcctcgatt gacagcgttt cgccgttgcc aaaaaccagt tcagcatcca gtgccaacag
    61861 gacgggaagc agcaccgact cttcctggcg ggcggcgatt tcaccaccaa tggtcgactg
    61921 attacgaaca tggcgtgagt aaacaaaacc gagggcttca cgcagcgctg caggaataaa
    61981 tcgcgcatca cgcagtggct gcaagcgaga cattgcgcca atccgcagtg caccgttatc
    62041 ccagtcaacc cagtccagtt ccagatcctg taaggaaatg gcaatctttt tatcggtacg
    62101 ggttggtgta gcattgagtt tgctgccccc ggcgaaccag acggcttcat cctggtagcg
    62161 gcgcttcagt tccagcgcct gttcgacaga gtcgggcctg aaaaattgtt caatcatctt
    62221 ctttccttat ttaaccctct tccctgcggg aagagggcat ctgtcattta tgccagcgca
    62281 tccatccgac gccacatact ggcagcggct tttctggctt gtgcataaat ggaatcgcaa
    62341 tcgaagttaa actgacggtc ttcatagacc atcacaccat tgaccattac gctgtgaacg
    62401 ctgcctgagc ccataccgaa agcgatatgc ccggcgatat tgtctgccag cagcggcgtc
    62461 ggcgagttgt aatcgcaaat ggttaaatca gctttgtaac cggcttccag aagcccaaat
    62521 ttcgcgccaa agttgcggct catcagttcg ttgccattag tcagggcttt ggcaaaactg
    62581 tcaggccaca gcggaccacc cgcatcgcga tgtttaaaga aggcaaattt catctcttca
    62641 aacatgtccg aaccaatgcc gtccgttccc aacgccagat tgcggatgtc gctaaggtga
    62701 tggttgtagc cgacatggtt gttcatgttt gaacgggcgt tatgcaccag gaacgcatcg
    62761 cgctgattga gtagggcgat gtcatctttc gacaagtaca gcccatgagc gaccagcgtt
    62821 ttgctatcga tgagatcgaa ttgcgccagt cgtgccagca ggtctttgcc gtaccagtgg
    62881 tgactgtagg aaacgtcgta aaggtcttcc gcagcgtgaa tatgcaaacc acggcctgtg
    62941 gctttcacgg cttcacgtag catctccaga ccggcatctg gcacggtaaa cggcgcgtga
    63001 gcaccgatat gtgcttccac cagatacggc tcggttgctg ctttcttcgc ctcatcaatc
    63061 tgacgggcga agcggatgtt ttcttctaca ccttcctgca actctttgat gccgttgtta
    63121 cggtcagtag tttcaaaaca ggtcatcgcg cgcaggccaa cttttaaaaa tgcgtcgcgc
    63181 aatgtggaga gcgacccgcc gatatacgcc ggagaggcat ggtgatcgat aaccgatgta
    63241 catccgctct taatcgcttc cagggaacaa atcagcccgc tgtaatagag cgactcttca
    63301 tcaagggcgc gatcgagccg ccaccagaga tttttcagcg ttgagatgaa atccgggcag
    63361 ggggcgatgt ttgccataat tccgcgggaa agccccgagt aaaaatggtt gtgcgagcag
    63421 acaattcccg gcatcacaat ccgaccatgc atctctttgt agctggcatc ggggtagcgt
    63481 tgcgtcaggg catcgccgat agcgacaatc acatcatttt cgatggcgat atcaacgcct
    63541 tcctgcactt tcgccgggtg taactgtact gcggtgacat tcttcagaat caacatgatt
    63601 acacctccac gcggcccagc agataatgat gatgctggtg gacatggctg atgatgcggc
    63661 acatatcgtt cagctccggt ggtacgttgt cgaactggcc ttcgctgtcg atgttcagca
    63721 cccagctttg gttattcaga cgtacgcgta cccgacaatc ttccaccagg aagcctgggt
    63781 tgctgctgtt atcaaagtct tgcgacaggc tgaagacggt gattttgtct ttgtacggtt
    63841 taccgttcca cggacagaac tgagcgcagt tgccgcattc gttacagtaa gcgtcgaggt
    63901 gcagcgtctg gaaacggttc tggaagcctg ggaccgcaat ggagacgttg gcgcggttcg
    63961 ggcagacatc cacacacttg ctgcaaacgt agttacattc gaggcagcga gcggcttcct
    64021 gcgcgacaaa cgcatcacga tcgtcactgt tcaccagcgt gatagagata tcgcctttac
    64081 gttgatagat ttccgccgga ttgacgttgt tccagtattt attgttctgg tgggaacgga
    64141 tattttcccg actaaggatg gcatcggtcg cccgacgcgc ggttccgaca gcagcgacaa
    64201 tggaggatgg tccgcgctgt acgtcgccga tcataaagac gtcagtcaga cgagtttcgc
    64261 cattatggtc gacgtctggc cagccgtttt tgtccagcgg tacgcccatc gcattcaggg
    64321 cttcagtatc ctgctgttca ccgatggcgg tgatcaggct gtctacatgc agtgttactg
    64381 tttcgttggt ttcaaccgga cggcgacgac ctttctcatc gggttcgcca agcgacataa
    64441 cgcgcaaggt taaggtgcca tcagcatcga aacattccgg attattcagg aaacggaact
    64501 ccacgccgtc gtgcaacgct tcttcatact cttcgcgcca tgcgggcatc tcttgcagtg
    64561 aacgacggta aacgatcgtt gctttttcta cgcctggaac gcgtaacgcc gcacgagcgc
    64621 agtccattgc ggtgttaccc gccccgacaa cgaccacatg tttgcccagc ttgagcgctg
    64681 tacccttgtt gtattcacgc aggaagggga gtgatttcca gacattttgg ttgtcgcccg
    64741 ccagtttcac accgctattt ttatcagtgc cggtggcaat cagaacatag tggaagccct
    64801 gattttttaa ctgttcaacg gtcaaatcgg gtgagcagcc atactcaaat ttcacgccgt
    64861 gagcggcaac aaaatcgata tcgtgctgaa ttaactctgc aggaatacgg aactgaggaa
    64921 tgatattttt caccacgccg ccagcattgg cttcgcgttc aaacagcgta accggatggc
    64981 ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca atcacggcaa
    65041 ccggatggcg tgaaccagaa ccggcgggtt tgtgccagcg ttgcttatat tcatcccaac
    65101 ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg taatccaggc
    65161 gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggcg ggcagagcgt
    65221 tgtgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc agacggatgt
    65281 attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg tagcaatcgg
    65341 tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt ttctgggtgt
    65401 attccatggt taacgcatct gctgccagtc tgttcagtcg ttcgacgtca acatggtcaa
    65461 gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc aaatagccgc
    65521 caggtttcag caggtcggtt gccatagtaa tagggcgaat accagtatca aaaatatcgc
    65581 ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg tcaaaggcgc
    65641 gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct gacatataca
    65701 tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta gtcagtttta
    65761 cgccaaagcc gagtgatttt tcttttgcca gtgccatcag gcgttccagc atttccagtg
    65821 cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg taaccgaaac
    65881 cgcagacatc gaggatctca cgaacacgcg cgtatcccag taaggtcggg ttaagtttca
    65941 caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg atttcatgcg
    66001 gaggacagcc gtgcatggtg gagagggtga cgccctgcac catactggtg gggatgcgag
    66061 cgggtaaggc ttgcaagctt tcgcgttttt cctgcaatcc gtggcgagct aaaaatgcgt
    66121 catcctggag taatttattc agcgtatcgc gatattgcgc gaatttcgga tggtcagatg
    66181 cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct tcgaggttgt
    66241 aaccgacgct catattaaag atgaacgatt taccggaatc agaaggctgg aacatcgctt
    66301 cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta agcagggtaa
    66361 attcggtaga ccattcggtg ttaaagcact cgtcttcggc atcgatacag ggcttttcca
    66421 gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg ccagtcagcc
    66481 aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg ccgacgggag
    66541 tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag taaaactgtt
    66601 gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg cgcgtcaaaa
    66661 gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta gcttatacag
    66721 atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga gcaacaatcg
    66781 caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggcggct cgaaacttaa
    66841 aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact cctgaaaatt
    66901 attattgtca gcccatttat cactttttct cttttctcaa aatcagtgat tccttatcat
    66961 tatgataaag agtgctggag agattaaata tttgaacgca atcgtttaac tacattttct
    67021 aaattttagt ataccttttt tgcgctgtaa taaagtcgtc cggagtatca atatctaaaa
    67081 ttatttctgc attttctatc tcgacggaat aatgagctcc cataaggagt gcctgacgca
    67141 tattggtgac attgggtcgt tggattgcct gcatcaagca tggttttgac actaaaatcg
    67201 gatggccggg gatgccatta tggagtggca gtattgcgcc atcatttcgt aacgaccaga
    67261 tttttctaaa aatatcgatg gtgagggttg gcatgtcacc gtgggtgaga aaacaatgtt
    67321 ctgtttgcac tgcgggtact gcggccttta ctgatgttag taaaccctgc gcataatctg
    67381 ggttgtggat aatagtgata ttgctctggt tcgcataacg ttcgtgcagt tcattaccac
    67441 gatagccggt gactaaaata attcggctac aaaactgcaa cgcatttttg atacttgtat
    67501 caagaattgt cccctgttgc cagggtaaca tcattttcca ttgccccatt cttgatgata
    67561 atccggcagc ggtaattata cagtcgatgg ctgacatagt ggttccatta tttatagggg
    67621 ctaataacgc ctgataattg cgttacttcc tgatggatta agttggggat aatttatccc
    67681 agagaggtca taaagactcg tgaaaagtat agttgaccca tcggcattag tcattgattt
    67741 aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt gctgggggga aaaccagctt
    67801 gcttttttgg ctggcagaac tgctccaggc tagcggcagg cgtgtgttaa tcacgacgac
    67861 gacacatatg tttatgccaa cgtctcactg gcccgtggtt ttctgtcgtg atcccgccat
    67921 gcttcctcat gcgtctctta catcccccat ttcattttgt tttcacagct ggaaagcgaa
    67981 ccagggaaaa gtgcagggat ttacgccaga agcgattgat gcactggtgc aacgaccaga
    68041 gtgtgacgta attctcattg aagcagatgg ctcgcgtgga atgccgttaa aagcgcctga
    68101 tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga tgggaggaca
    68161 tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt ttgctgatat
    68221 taccgggtta acacctgatg caaccttgca actgagcgat ctcgttgcgc tggttcgcca
    68281 ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt tcattaaccg
    68341 tttttctcaa tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc tgcaacaaca
    68401 cgacgtagag gcaatctggc tgggcgatat acaagaacat cctgcaatcg cgcgcagatt
    68461 tgtgaattag cggcccgggt attccgggtt actgaaagga tatccattga ggttggtatg
    68521 aatattttca cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc gatggcgcaa
    68581 attgttgata gccgaggctc gactccccgc cattctgcac aaatgttagt gcgcgccgat
    68641 ggttctatcg tcggtacaat tggtggcgga atggttgagc ggaaggtgat tgaagagtca
    68701 cttcaggcat tgcaggaacg taagccgcga ttattccatg gacgtatggc tcgtaacggt
    68761 gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag cgtccatggt
    68821 atgcgtccac gtctggtgtt gattggcgcg gggcatgtca accgggcgat agcccagagt
    68881 gccgcgctat taggatttga tattgccgtt gccgatattt atcgcgaaag cctcaatcct
    68941 gaactattcc caccatcaac cacgcttctc catgctgagt cgtttggtgc ggcagtggaa
    69001 gcactggata ttcgccctga taattttgtc ctgattgcca cgaataatca ggatcgtgaa
    69061 gccctcgaca aactcattga acagcccatt gcatggttgg ggttgctggc aagtcgtcgc
    69121 aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga acatattgcc
    69181 cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga gatcgccatc
    69241 agcgtgctgg cagaaatatt acaggtgaaa aataacgcgc cgggtgggct gatgatgaaa
    69301 ccttctcatc cttccggaca ccagctggtg gtgattcgcg gtgcgggaga tatcgccagt
    69361 ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga tcatgctgga agtggaaaaa
    69421 ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg cgaaatgacg
    69481 gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg aagcgatgaa acttaccgaa
    69541 cgcggattca tccctgtgat ggtagatccc gcctgttcat tgcttgatga actgaaaccg
    69601 ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg ggacgcgggc agatatggca
    69661 ccagtaacaa tcgcgcttgg gccgggcttt attgcaggga aggattgtca tgcggtaatt
    69721 gaaacaaatc gcgggcactg gctcggtcag gtgatttact ctggttgtgc gcaggagaat
    69781 accggtgttc ctggcaatat tatggggcat accacccgac gggtgatccg tgctcctgct
    69841 gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg cgatgtgatt
    69901 gcctggattg gtgagcatga aattaaagca ccgttgacgg ggatggtgcg tggcttgttg
    69961 aacgacggcc tggcagtggt tggtggtttt aaaattggtg atatcgatcc tcgtggtgaa
    70021 accgctgatt tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg cgtacttgag
    70081 gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct ggaagtggct
    70141 taaatgaaaa tggccggatg gtattaccca cccggcctta aaattactga caaatcaccg
    70201 ttccggtttt gccttcaatc ccttctttcg ctttgctcaa tacggtaatc agtgcttcac
    70261 gacctgcgcg agagcgagca aacgaggcgg cagcttccac tttaggcagc atagaacctt
    70321 tcgcaaaatg accttcttcg ataaagcgtt cagcatcact taatgacagg cgatcgagcc
    70381 actgttcatt ctctttaccg aagttaatgg ccactttttc taccgcagtc agaatgatca
    70441 gcatatcggc atcaatcatt tctgctaaac gggcgctggc ccagtcttta tcgataaccg
    70501 cgctggcacc acgcagatgg ttaccttcac gaataacagg gataccacca ccgccaacgg
    70561 tgatcaccac ctggcctgca tctaccagag ctttaaccgt ttctttttca atgatatcaa
    70621 ccggttttgg cgaggcaaca acacggcgat agccgcgacc agcatcttct ttcagagtgt
    70681 aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg atcggcttgg
    70741 tcgggttgag gaatgccgga tcgttagcgt cgacttcgac ctgagtaacc agcgttgcta
    70801 caggtttatt aatgccccga gaaagcagtt cttctcttaa cgcgttttgt agatcgtaac
    70861 caatataacc ctggcttaag gcaacacaga cagacatcgg cagcattggc gagtgtgctt
    70921 cagtttttgc ggcagcttca aaggcctggt taatcatgcc cacctgtggc ccgttaccgt
    70981 gagtgacgat gacctcatgt ccctgggcaa ttaaatcaac aattgcctga gaagtaattt
    71041 ttaccgcttt catttgcccg gccagatcat cgcccagcgc attcccgccc agggcgagaa
    71101 caattttctt actcattttt cctcactcat cattcttgat tgtgtataga tccctggcag
    71161 tggttagagc acgggaggga caaacggttt gcgtcgcagg aaacgacctc gcccggcttt
    71221 gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc tgacaatcgc
    71281 gccctgacag gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga gatggcgatg
    71341 ctgaatttgt tggctctgac gtgggtcgat aatcaccacg tcgccatcgg aaccgggcgc
    71401 taataatcct ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc tggttaattc
    71461 aacaaagcgt tccggtgtta tacgtcccgt catcacgcca ctggaaaaca gtaactgcat
    71521 gcggttttcc acgccgggta agccatttgg gcagcgactg aaatcgcctt tagaaatttg
    71581 caggcgttga gccattgaga acgtgcagtg atcggtcgcc accacgtcaa tcgcaccatc
    71641 gctgatgcca caccacagtt tgtcctgctc gcgtatgtta cgcagcggtg ggctaagaat
    71701 gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc aacaggagat attgtgggca
    71761 ggtttcaacc cagactggct ggtgattcgc acgggcaaga cgcagataat ccagacctaa
    71821 gccgttagac aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg ccaggttaat
    71881 catgcgggcg atggcttccg cttcgcattc cagagggcga ctcaaggcat gatagcgcgg
    71941 cgcggttaac cctgcggcga taaattccgc ccgcttgctg gcgatagccg catcattttc
    72001 cgggtgcacg gtggtcagcg cgccggattc atgcagacgg cgtaatgcct gcaaaacctc
    72061 gtcatcgttg agtttgtatt gataggttaa atagagttta aaactgctca gtccttcctc
    72121 gaccatcatc gggatttcgt cgaggattgc gtgattaatg tgctggatca caccgtgaaa
    72181 gctgtaatcg atgaccgctt tatgggcggc ataatcacga taaacctcca gttgatggcg
    72241 taaccgacag ccgtttgggc caaatcccat atggtcaata atggttgttg taccgccaca
    72301 cacagctgcg cgggtaccgg taaaaaaatc atcacaactg cgcgcgatgc cgacatcaat
    72361 attgaaatgc gtatggacat ccacgccgcc agggaaaacg taacagccag tggcatcaat
    72421 ttcttcatac gggagctgcg gcgaaatatt gttgcccaac tggcgaacaa tcccgctttc
    72481 aatcagcaaa tcctgtttgg cttgtccatc tgcgttaacg acagtgccgt ttttgatcaa
    72541 tacgcgcata gcaaactcca aaaaccctcc ggcgaaccgg aggggaataa gtgaagagta
    72601 attactctgt tgccagccag cttaacggga ttgcagcgta catcgcggca caggtcacca
    72661 ggtgagattt ccaggttttt tcgttaggtg catgcgcttc aggttcttta cccgggccaa
    72721 agccgatcac cggaatgccg tgacggccca tgatagatac gccgttagtt gagaaggtcc
    72781 acttatcaac aaccggcgct ttgccaaaca gcccttcgta ggcattcacc agtgctttaa
    72841 cggtgaagtg atcttcttcc actttccagg tcgggaagta gcattcggtt gggtaaacca
    72901 ggccagtcca ggacggacgg tcgtagttgt acatagaaac aaccgcgtta gctttctgta
    72961 ctgcaggcag ggcgcggatt tcgtccagcg cgccttccca ggtttcgccc caggtcagac
    73021 ggcggtcaat agagacggcg cagctatctg ctacagcgca acggcttggg gatgtgaaga
    73081 agatttcaga aacggtgagg gtgcctttgc cgaggaattc gtcataaccc agacgttggg
    73141 agagttcttg taattcgcca agaatcggac ccattttgaa aatggcgttg tcaccgcgtt
    73201 ctggcgcaga accgtggcag ctaacaccct gaacatcaat acgaatttcc atacgaccgc
    73261 gctgaccacg gtatatctgg cagtcggttg gttcggtact gaccacaaat tccgggcgaa
    73321 tgccggattg ttcaataatg tactgccagc acagaccgtc gcagtcttct tcctgcacag
    73381 taccggtaac cagcagggta tattcatctt ccagaccgag gtctttaatg attttaccgg
    73441 cataaaccat agatgccatg ccgccttcct ggtcggaagt accgcgacca ccgattagct
    73501 catcagtttc catgccttcg tacggatcga agtcccagtt tttgatgttg ccaatgccga
    73561 cggtatcgat atgagcgtcc attgccacca gacgcgggcc gtggccgata taaccgagaa
    73621 cgttgcccat cgggtcgatt tcaactttat cgaagccgac tttttccatc tcttctttaa
    73681 tacgatgtac tacgcgtttt tcgtcgcagc tttcactggg aatagcaacc atgtcgcgca
    73741 ggaagcgagt catgtccgcc tggtaatctt ttgctttttc aagaatcagt ttgaatggaa
    73801 tattcttagc cattattttt ctccagattt tttactcccc gttttgcagg gagtagttcc
    73861 aatagggtga ttaaggtgct acagcgtgtt tgccttccca gacaacttcg cggtagtgct
    73921 tcacgtcggt gtcgccttca gtgctgataa ccagcaccac ggcatctttg ttcagcgcca
    73981 gtttttccat caggctttga cgttgcgggt gataatgaac cgccgcgaga acgcccaaac
    74041 cgacagcgcc ggattcaccg gagatgatgc gcgggtcgtt gccgtacgga ttacccagca
    74101 cgcgcatacc taatgcggca acgctgtcct ggcaggagat gaattgggtg gcgcagttac
    74161 gtaggatttc ccagcccagc gggttaggtt cgccacaggc caggcccgcc atgatggtgg
    74221 ccatatcacc gccaacgttg acgatgtcgc ctttgacgcc ggagcgataa atacagtcag
    74281 ctttgtcagg ttcaacaata atgctgtgca gattttgcgg gctatagacg tcgaccagat
    74341 aacccagcac accaccggcc attgctccga caccggcttg cagcagaacg tgcgtcgggg
    74401 ttacgcccat ttcacgcatt tgctcgacgg tttcatctgc cagggttgcg tagccttgca
    74461 tgatccaggt tgggattttg gtgtaacctt cccatgccgt gtcctgtacc acttcccagc
    74521 cgtgctgctg cgcgtgttgc atggtcaggc gaacggtatc gtcatagttc atatccgtga
    74581 cgatgcactc ggcaccgagg ttcagaatgg cgtcaacgcg ttcctgagca gaacctttcg
    74641 gcatgtaaat caccgcattc tgtccgagtt gctgcgctgc ccacgccaca ccgcgcccgt
    74701 ggttgccgtc ggtggtggtc gcgaaagtca ttttttcgcc gatggcattt ttcaggtgct
    74761 caaatgacag cgtttcgata tcaagatgat atttttcaca caataattga gcgatggcgt
    74821 acgcaccgcc gagcattttg aaggcgttca gaccgaatcg ttttgattcg tctttgacaa
    74881 gaattttctt cacaccaaaa aggtttgcga gatcgtccag cgcacaaagc ggtgttgggc
    74941 gataaccagc tattttctgg tggaactggc gcgccagttt ggcctggctt tgcgaaaaga
    75001 gcggtgatgt ttcgccgttg aaaaatttgt tatcggcgat atcaatcttc aatgagaaaa
    75061 cggacatatc ctatcctttt ggtctctgga aatagagggc cgaccagctg atcggcccga
    75121 ggggttattt cacgcgttct tgcgcttctt tcagcagttg ctcgagcagt gcacctggtt
    75181 tggcgtattt acgggacagg atcatcgcgg cgatgatata aggcttccag ctggcttctt
    75241 tgtaggttgc gatacggtat ttttcgaata cgccttcagt cacttcacct tctttacagg
    75301 atacgccgct gatatcagct ggcaggcagt gcatgtacag ggcttcgcca tcacgggtca
    75361 gttccatcat ctcttcagta caatgccagt ctttgtgttg cgcgttctgt gccagacact
    75421 gtttttccag tgcttttaag ccttcgtgat cgttagcgcg cagcaattcg gtacgctgtt
    75481 ccatcacttt gtaaggtgcc catgacttcg gataaacgat gtctgcatct ttgaacgctt
    75541 cttccatgct ggtgacctga cggaagctac cgccagatgc tttagcattg tttttcgcca
    75601 cttcaaccac atccgggatc aggtcgtagc cttccggatg ggccagggtg acatccatac
    75661 cgaagcgagt catcagaccg atgatgcctt gtggtacaga gagcggtttg ccatagcttg
    75721 gagagtaggc ccaggtcatg gcgatttttt tacctttcag gttttccagt gaaccaaagt
    75781 gttcacgtaa ccacgcgagg tcagccattg actgagtcgg gtggtcaata tcgcattgca
    75841 ggttcactaa agccggacgc tgtggcagta caccctgctt gtaaccgtca tcaagtgcag
    75901 cgccaacttc acgcatatag gcgttgcctg cgcccagata catatcgtcg cgaataccaa
    75961 tagcgtcggc gcagaaggag atcatattgg cggtttcacg cacggtttcg ccgtgagcga
    76021 tttgtgattt gccttcgtcg agatcttgtt gtgcaaggcc gagcaggtta agcgcggaag
    76081 cataagagaa gcgggtacgg gtggagttgt cgcggaatac ggaaatacct aatccactat
    76141 taaagacttt ggttgagatg ttttcagcac gcagtgcttt taatgctgcg gcaacgtcca
    76201 gtacttgttt cagttcatct ggcgtctgtt cccacgttaa caaaaaatct ttctcgtgaa
    76261 ggtgagaggt cagcgaattg atatccttaa tcagctcatt aacagttttc atcataaagt
    76321 cccggtagtg gaatgacagg caaagtggtt tatttcactg tgacttgcca aagtgattgg
    76381 tgttttcaca gctatacctg ggactaacaa taagtgtgcc agaattatcc acggggctgc
    76441 aaaagactaa tttgacggat aatgtgtggg gttgatcaca aattgatgcc agcttccggt
    76501 tgtttgtaaa atacgaaaac gtttggcttt ttttatcgcg ttatttattt gctgctaagt
    76561 atcagaaata agataaaggg gcaggctatc tctcttcagt ttgataaact
//
LOCUS       DAPGWD010000002        75262 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPGWD010000002 DAPGWD010000000
VERSION     DAPGWD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.002"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(59..1309)
                     /gene="icd"
                     /locus_tag="RHX88_000074"
     CDS             complement(59..1309)
                     /gene="icd"
                     /locus_tag="RHX88_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDV2228810.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1481..2134
                     /gene="rluE"
                     /locus_tag="RHX88_000075"
     CDS             1481..2134
                     /gene="rluE"
                     /locus_tag="RHX88_000075"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDV2228811.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2144..2605
                     /gene="nudJ"
                     /locus_tag="RHX88_000076"
     CDS             2144..2605
                     /gene="nudJ"
                     /locus_tag="RHX88_000076"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDV2228812.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2659..3765
                     /gene="mnmA"
                     /locus_tag="RHX88_000077"
     CDS             2659..3765
                     /gene="mnmA"
                     /locus_tag="RHX88_000077"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDV2228813.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3801..4442
                     /gene="hflD"
                     /locus_tag="RHX88_000078"
     CDS             3801..4442
                     /gene="hflD"
                     /locus_tag="RHX88_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDV2228814.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4446..5816
                     /gene="purB"
                     /locus_tag="RHX88_000079"
     CDS             4446..5816
                     /gene="purB"
                     /locus_tag="RHX88_000079"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDV2228815.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5984..6655
                     /gene="phoP"
                     /locus_tag="RHX88_000080"
     CDS             5984..6655
                     /gene="phoP"
                     /locus_tag="RHX88_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDV2228816.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6655..8115
                     /gene="phoQ"
                     /locus_tag="RHX88_000081"
     CDS             6655..8115
                     /gene="phoQ"
                     /locus_tag="RHX88_000081"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDV2228817.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8191..9312
                     /gene="roxA"
                     /locus_tag="RHX88_000082"
                     /gene_synonym="ycfD"
     CDS             8191..9312
                     /gene="roxA"
                     /locus_tag="RHX88_000082"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDV2228818.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9458..10687)
                     /gene="pepT"
                     /locus_tag="RHX88_000083"
     CDS             complement(9458..10687)
                     /gene="pepT"
                     /locus_tag="RHX88_000083"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDV2228819.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGNIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10937..12073
                     /gene="potA"
                     /locus_tag="RHX88_000084"
     CDS             10937..12073
                     /gene="potA"
                     /locus_tag="RHX88_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDV2228820.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12057..12914
                     /gene="potB"
                     /locus_tag="RHX88_000085"
     CDS             12057..12914
                     /gene="potB"
                     /locus_tag="RHX88_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDV2228821.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12911..13705
                     /gene="potC"
                     /locus_tag="RHX88_000086"
     CDS             12911..13705
                     /gene="potC"
                     /locus_tag="RHX88_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDV2228822.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13702..14748
                     /gene="potD"
                     /locus_tag="RHX88_000087"
     CDS             13702..14748
                     /gene="potD"
                     /locus_tag="RHX88_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDV2228823.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14806..15267
                     /locus_tag="RHX88_000088"
     CDS             14806..15267
                     /locus_tag="RHX88_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDV2228824.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPYTKKSRRKRKSCP"
     gene            15264..16052
                     /locus_tag="RHX88_000089"
     CDS             15264..16052
                     /locus_tag="RHX88_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDV2228825.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16191..17012)
                     /gene="cobB"
                     /locus_tag="RHX88_000090"
     CDS             complement(16191..17012)
                     /gene="cobB"
                     /locus_tag="RHX88_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDV2228826.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17028..17939)
                     /gene="nagK"
                     /locus_tag="RHX88_000091"
     CDS             complement(17028..17939)
                     /gene="nagK"
                     /locus_tag="RHX88_000091"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDV2228827.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17968..19212)
                     /gene="lolE"
                     /locus_tag="RHX88_000092"
     CDS             complement(17968..19212)
                     /gene="lolE"
                     /locus_tag="RHX88_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDV2228828.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19212..19913)
                     /gene="lolD"
                     /locus_tag="RHX88_000093"
     CDS             complement(19212..19913)
                     /gene="lolD"
                     /locus_tag="RHX88_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDV2228829.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19906..21105)
                     /gene="lolC"
                     /locus_tag="RHX88_000094"
     CDS             complement(19906..21105)
                     /gene="lolC"
                     /locus_tag="RHX88_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDV2228830.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21367..22440
                     /locus_tag="RHX88_000095"
     CDS             21367..22440
                     /locus_tag="RHX88_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV2228831.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQQFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLGYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAISYSR"
     gene            22568..26014
                     /gene="mfd"
                     /locus_tag="RHX88_000096"
     CDS             22568..26014
                     /gene="mfd"
                     /locus_tag="RHX88_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDV2228832.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGLDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKSGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26158..27120
                     /gene="ldtC"
                     /locus_tag="RHX88_000097"
     CDS             26158..27120
                     /gene="ldtC"
                     /locus_tag="RHX88_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDV2228833.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27203..27460)
                     /gene="bhsA"
                     /locus_tag="RHX88_000098"
     CDS             complement(27203..27460)
                     /gene="bhsA"
                     /locus_tag="RHX88_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDV2228834.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            27701..28333
                     /gene="comR"
                     /locus_tag="RHX88_000099"
     CDS             27701..28333
                     /gene="comR"
                     /locus_tag="RHX88_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDV2228835.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(28395..28934)
                     /locus_tag="RHX88_000100"
     CDS             complement(28395..28934)
                     /locus_tag="RHX88_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV2228836.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(29144..30448)
                     /gene="ndh"
                     /locus_tag="RHX88_000101"
     CDS             complement(29144..30448)
                     /gene="ndh"
                     /locus_tag="RHX88_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDV2228837.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(30848..31390)
                     /locus_tag="RHX88_000102"
     CDS             complement(30848..31390)
                     /locus_tag="RHX88_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2228838.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(31413..32438)
                     /gene="nagZ"
                     /locus_tag="RHX88_000103"
     CDS             complement(31413..32438)
                     /gene="nagZ"
                     /locus_tag="RHX88_000103"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDV2228839.1"
                     /translation="MGPVMLDVKGYELEAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(32449..33273)
                     /gene="thiK"
                     /locus_tag="RHX88_000104"
     CDS             complement(32449..33273)
                     /gene="thiK"
                     /locus_tag="RHX88_000104"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDV2228840.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFHSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKVREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(33254..33895)
                     /gene="lpoB"
                     /locus_tag="RHX88_000105"
     CDS             complement(33254..33895)
                     /gene="lpoB"
                     /locus_tag="RHX88_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDV2228841.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(33909..34286)
                     /locus_tag="RHX88_000106"
     CDS             complement(33909..34286)
                     /locus_tag="RHX88_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDV2228842.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(34289..34648)
                     /gene="hinT"
                     /locus_tag="RHX88_000107"
     CDS             complement(34289..34648)
                     /gene="hinT"
                     /locus_tag="RHX88_000107"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDV2228843.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            34982..37171
                     /gene="fhuE"
                     /locus_tag="RHX88_000108"
     CDS             34982..37171
                     /gene="fhuE"
                     /locus_tag="RHX88_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDV2228844.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTQGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(37231..38664)
                     /gene="ptsG"
                     /locus_tag="RHX88_000109"
     CDS             complement(37231..38664)
                     /gene="ptsG"
                     /locus_tag="RHX88_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDV2228845.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(38959..39756)
                     /locus_tag="RHX88_000110"
     CDS             complement(38959..39756)
                     /locus_tag="RHX88_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2228846.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMWDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(39767..40771)
                     /gene="holB"
                     /locus_tag="RHX88_000111"
     CDS             complement(39767..40771)
                     /gene="holB"
                     /locus_tag="RHX88_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDV2228847.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(40768..41409)
                     /locus_tag="RHX88_000112"
     CDS             complement(40768..41409)
                     /locus_tag="RHX88_000112"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDV2228848.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(41399..42421)
                     /gene="yceG"
                     /locus_tag="RHX88_000113"
     CDS             complement(41399..42421)
                     /gene="yceG"
                     /locus_tag="RHX88_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDV2228849.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(42424..43233)
                     /gene="pabC"
                     /locus_tag="RHX88_000114"
     CDS             complement(42424..43233)
                     /gene="pabC"
                     /locus_tag="RHX88_000114"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDV2228850.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(43353..44594)
                     /gene="fabF"
                     /locus_tag="RHX88_000115"
     CDS             complement(43353..44594)
                     /gene="fabF"
                     /locus_tag="RHX88_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDV2228851.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(44682..44918)
                     /gene="acpP"
                     /locus_tag="RHX88_000116"
     CDS             complement(44682..44918)
                     /gene="acpP"
                     /locus_tag="RHX88_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV2228852.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(45129..45863)
                     /gene="fabG"
                     /locus_tag="RHX88_000117"
     CDS             complement(45129..45863)
                     /gene="fabG"
                     /locus_tag="RHX88_000117"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDV2228853.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(45876..46805)
                     /gene="fabD"
                     /locus_tag="RHX88_000118"
     CDS             complement(45876..46805)
                     /gene="fabD"
                     /locus_tag="RHX88_000118"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV2228854.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(46821..47774)
                     /gene="fabH"
                     /locus_tag="RHX88_000119"
     CDS             complement(46821..47774)
                     /gene="fabH"
                     /locus_tag="RHX88_000119"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDV2228855.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(47842..48912)
                     /gene="plsX"
                     /locus_tag="RHX88_000120"
     CDS             complement(47842..48912)
                     /gene="plsX"
                     /locus_tag="RHX88_000120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDV2228856.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDVRPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(48993..49166)
                     /gene="rpmF"
                     /locus_tag="RHX88_000121"
     CDS             complement(48993..49166)
                     /gene="rpmF"
                     /locus_tag="RHX88_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDV2228857.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(49218..49739)
                     /gene="yceD"
                     /locus_tag="RHX88_000122"
     CDS             complement(49218..49739)
                     /gene="yceD"
                     /locus_tag="RHX88_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDV2228858.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            49938..50522
                     /gene="yceF"
                     /locus_tag="RHX88_000123"
     CDS             49938..50522
                     /gene="yceF"
                     /locus_tag="RHX88_000123"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDV2228859.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(50634..51593)
                     /gene="rluC"
                     /locus_tag="RHX88_000124"
     CDS             complement(50634..51593)
                     /gene="rluC"
                     /locus_tag="RHX88_000124"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDV2228860.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            52166..55351
                     /gene="rne"
                     /locus_tag="RHX88_000125"
     CDS             52166..55351
                     /gene="rne"
                     /locus_tag="RHX88_000125"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDV2228861.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(55547..56500)
                     /gene="flgL"
                     /locus_tag="RHX88_000126"
     CDS             complement(55547..56500)
                     /gene="flgL"
                     /locus_tag="RHX88_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDV2228862.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(56512..58155)
                     /gene="flgK"
                     /locus_tag="RHX88_000127"
     CDS             complement(56512..58155)
                     /gene="flgK"
                     /locus_tag="RHX88_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDV2228863.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(58221..59162)
                     /gene="flgJ"
                     /locus_tag="RHX88_000128"
     CDS             complement(58221..59162)
                     /gene="flgJ"
                     /locus_tag="RHX88_000128"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDV2228864.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(59162..60259)
                     /gene="flgI"
                     /locus_tag="RHX88_000129"
     CDS             complement(59162..60259)
                     /gene="flgI"
                     /locus_tag="RHX88_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDV2228865.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(60271..60969)
                     /gene="flgH"
                     /locus_tag="RHX88_000130"
     CDS             complement(60271..60969)
                     /gene="flgH"
                     /locus_tag="RHX88_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDV2228866.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(61022..61804)
                     /gene="flgG"
                     /locus_tag="RHX88_000131"
     CDS             complement(61022..61804)
                     /gene="flgG"
                     /locus_tag="RHX88_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDV2228867.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(61976..62731)
                     /gene="flgF"
                     /locus_tag="RHX88_000132"
     CDS             complement(61976..62731)
                     /gene="flgF"
                     /locus_tag="RHX88_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDV2228868.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(62751..63956)
                     /gene="flgE"
                     /locus_tag="RHX88_000133"
     CDS             complement(62751..63956)
                     /gene="flgE"
                     /locus_tag="RHX88_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDV2228869.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(63981..64676)
                     /gene="flgD"
                     /locus_tag="RHX88_000134"
     CDS             complement(63981..64676)
                     /gene="flgD"
                     /locus_tag="RHX88_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDV2228870.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(64688..65092)
                     /gene="flgC"
                     /locus_tag="RHX88_000135"
     CDS             complement(64688..65092)
                     /gene="flgC"
                     /locus_tag="RHX88_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDV2228871.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(65096..65512)
                     /gene="flgB"
                     /locus_tag="RHX88_000136"
     CDS             complement(65096..65512)
                     /gene="flgB"
                     /locus_tag="RHX88_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDV2228872.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            65667..66326
                     /gene="flgA"
                     /locus_tag="RHX88_000137"
     CDS             65667..66326
                     /gene="flgA"
                     /locus_tag="RHX88_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDV2228873.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            66402..66695
                     /gene="flgM"
                     /locus_tag="RHX88_000138"
     CDS             66402..66695
                     /gene="flgM"
                     /locus_tag="RHX88_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDV2228874.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            66700..67116
                     /gene="flgN"
                     /locus_tag="RHX88_000139"
     CDS             66700..67116
                     /gene="flgN"
                     /locus_tag="RHX88_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDV2228875.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     HNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(67156..68691)
                     /gene="murJ"
                     /locus_tag="RHX88_000140"
     CDS             complement(67156..68691)
                     /gene="murJ"
                     /locus_tag="RHX88_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDV2228876.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(68801..69724)
                     /locus_tag="RHX88_000141"
     CDS             complement(68801..69724)
                     /locus_tag="RHX88_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV2228877.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(69726..70373)
                     /locus_tag="RHX88_000142"
     CDS             complement(69726..70373)
                     /locus_tag="RHX88_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDV2228878.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(70384..70968)
                     /gene="rimJ"
                     /locus_tag="RHX88_000143"
     CDS             complement(70384..70968)
                     /gene="rimJ"
                     /locus_tag="RHX88_000143"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDV2228879.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            71204..72412
                     /gene="mdtH"
                     /locus_tag="RHX88_000144"
     CDS             71204..72412
                     /gene="mdtH"
                     /locus_tag="RHX88_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDV2228880.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGCSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            72476..73123
                     /gene="grxB"
                     /locus_tag="RHX88_000145"
     CDS             72476..73123
                     /gene="grxB"
                     /locus_tag="RHX88_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDV2228881.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            73257..73817
                     /locus_tag="RHX88_000146"
     CDS             73257..73817
                     /locus_tag="RHX88_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2228882.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            73923..74969
                     /gene="pyrC"
                     /locus_tag="RHX88_000147"
     CDS             73923..74969
                     /gene="pyrC"
                     /locus_tag="RHX88_000147"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDV2228883.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            75043..>75262
                     /gene="dinI"
                     /locus_tag="RHX88_000148"
     CDS             75043..>75262
                     /gene="dinI"
                     /locus_tag="RHX88_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDV2228884.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWES"
BASE COUNT        18274 a        20033 c        18836 g        18119 t
ORIGIN      
        1 cataaataaa acaacgggcg tgttatacgc ccgttgcaat attaaacaca tgtagtgatt
       61 acatgttctt gatgatcgca tcaccaaatt ctgaacattt cagcagttta gcgccttcca
      121 tcagacgttc gaagtcatag gttacagtct tggcgttgat tgcgccttcc atacctttaa
      181 caatcaggtc tgcggcttca gtccagccca tatggcgtaa catcatctca gcggagagaa
      241 taatagagcc agggtttact ttgtcctgac cggcgtattt cggcgcagta ccgtgggtgg
      301 cttcaaacag ggcgcattcg tcaccgatgt ttgcaccagg ggcgataccg ataccgccaa
      361 cctgcgctgc cagggcgtca gaaatgtagt caccgttcag gttcatacag gcgataacat
      421 catattcagc cggacgcagc aggatctgtt gcaggaatgc atcagcaatc acgtctttaa
      481 tgacgatctc tttgccggtg ttcgggtttt taactttcag ccacgggccg ccgtcgatca
      541 gttcaccgcc aaactcttca cgcgccagct ggtagcccca gtctttaaac gcgccttcgg
      601 tgaacttcat gatgttgcct ttgtgcacca gggtcacaga gtcacggtcg ttggcaattg
      661 cgtattcaat cgcggcacgg accagacgtt tggtgccttc ttcagaacac ggcttgatac
      721 cgataccgca atgttccggg aagcgaattt tcttcacgcc catctcttca cgcaggaatt
      781 taatcacttt ctcggcgtcg gcagagtctg ctttccattc gatacccgca taaatgtctt
      841 ccgagttttc acggaagata accatatcgg tcagttcagg gtgtttaacc gggcttggag
      901 tgccttgata gtaacgtacc ggacgcaggc agatgtagag atccagttcc tggcgcaggg
      961 caacgttcag agagcgaata ccgccaccaa caggagtggt cagcggacct ttaatggcaa
     1021 cgcgatattc acgaatcaga tcaagggttt cagcaggcag ccagacgtcc tgaccataaa
     1081 cctgtgtgga tttttcaccg gtgtaaattt ccatccagga gattttacgc tcgcctttat
     1141 aggctttctc gactgcagcg tcgaccactt tcagcatggc tggggttaca tctacaccga
     1201 ttccatcacc ttcaatgtaa gggataatcg gattttcagg aacgttgagt ttgccgtttt
     1261 gcagggtgat cttcttgcct tgtgccggaa caactacttt actttccatt cacctctcct
     1321 tcgagcgcta ctggtttgct cgtctgccac cacgttgcat atgcgtttgc gtcctgcgat
     1381 acggatgctt tagagcaatt ttttgttaat gatttgtaat tggcttgtca atactaccct
     1441 attgtttgcc gtcatgaaag atgcgcgtta ttaggctata atgcggcaat tcataatctc
     1501 tgaaaatacc atgcaaaaaa cttcttttag aaatcaccag gttaagcgat tcagctcgca
     1561 acgttctacc aggcgtaaac ctgaaaacca gcccacgcgt gtgatcctgt tcaataaacc
     1621 ctacgatgtt cttccgcagt tcaccgatga agccggacgc aaaacattaa aagaattcat
     1681 cccggttcag ggcgtttatg cagcaggtcg tcttgaccgc gatagcgaag ggttactggt
     1741 gctgaccaat aacggcgcgt tgcaggcgcg tttaacccag ccgggtaaac gcaccgggaa
     1801 aatctattat gtgcaggtgg aaggtattcc cacacaagac gcacttgaag ccttgcgcaa
     1861 tggcgtaacc ttaaatgatg gccctaccct gcccgccggc gcggagctgg ttgacgaacc
     1921 agcgtggtta tggccgcgga atccaccaat tcgtgaacgc aaaagtattc ccaccagctg
     1981 gctgaagatc accttatatg aaggacgtaa tcgccaggtg cgccgcatga ccgcccatgt
     2041 tggtttcccc acgctgcgac tgattcgcta tgcgatggga gattactctt tggataatct
     2101 tgccaatggc gaatggcgag aagtgacaga ttaaggatgc aaaatgttta aaccgcacgt
     2161 taccgttgct tgcgtggtgc acgcagaagg caaattttta gtcgttgaag agacgattaa
     2221 tggtaaagcg ttatggaacc aacctgccgg gcatctggaa gccgatgaaa ccttagtgga
     2281 agccgccgcc cgtgaactgt gggaagaaac cggcatcagc gcgcagccgc aacactttat
     2341 tcgtatgcat cagtggattg cgccagataa aacgccgttt ttgcgtttcc tctttgccat
     2401 tgagcttgag caaatatgcc cgacccagcc tcatgacagc gatatcgact gctgtcgttg
     2461 ggtcagcgcc gaagagattt taaaggcgtc aaatcttcgt tcgccgctgg tggcggaaag
     2521 tatccgttgt tatcaaagcg ggcaacgcta tccgctggag atgattggcg attttaactg
     2581 gccttttaca aagggtgtca tctaaaaggc tgcgtgatag aatacgccgc cttgaagttc
     2641 aatgtcgtga gtgatccaat gtctgaaacc gcaaaaaaag taatcgtcgg catgtccggc
     2701 ggtgtcgatt cctccgtttc tgcctggctg ttgcaacaac agggatatca ggttgaaggc
     2761 ctgtttatga agaactggga agaagacgac ggtgaggaat attgcacagc ggcagcggat
     2821 ctggctgatg cccaggctgt ctgcgacaag ctcggcattg aactgcacac cgttaacttt
     2881 gctgccgagt actgggacaa cgtcttcgaa ctgttccttg ccgaatataa agccggtcgc
     2941 acgccgaatc cggatattct gtgcaacaaa gagatcaaat ttaaagcctt cctcgaattt
     3001 gccgccgaag atttaggtgc cgattatatc gctaccggtc attacgtacg tcgggccgat
     3061 gtcgatggca agagccgcct gctgcgtggt ctggacagca ataaagacca gagctacttc
     3121 ctttatacgc tcagccatga gcagattgcg caaagcctgt tcccggtcgg cgaactggaa
     3181 aaaccgcagg tgcgtaagat tgctgaagat cttggtctgg tcaccgcgaa gaaaaaagac
     3241 tctaccggca tttgcttcat tggcgaacgt aaattccgcg agttcctggg ccgttatctc
     3301 ccggcgcaac cgggcaaaat cattaccgtc gatggcgatg aaattggtga gcaccagggg
     3361 ctgatgtatc acactctcgg tcagcgtaaa ggtctgggta tcggtggcac caaagaaggt
     3421 accgaagaac cgtggtatgt ggtggacaaa gacgtcgaaa acaacattct ggttgtcgct
     3481 cagggccatg aacacccgcg gctgatgtct gtcgggttga ttgcccagca gttgcactgg
     3541 gtcgatcgcg aaccattcac cggcactatg cgttgcacgg taaaaacccg ctatcgccag
     3601 accgatatcc cttgcaccgt caaggcgctg gacgatgatc gcattgaagt gattttcgat
     3661 gaaccggttg ccgccgtgac gccgggccag tctgccgtct tctataacgg tgaagtgtgc
     3721 ctcggtggcg gtattattga gcagcgtctg ccgctgccgg tctgattatt atctttactt
     3781 aaaacaagga agcagtgaac gtggcaaaga attactatga catcaccctc gccctggccg
     3841 gtatttgtca gtcggcacgc ctggtgcaac aactcgctca ccagggacat tgtgatgccg
     3901 atgcgctaca cgtctcactc aacagtatta ttgatatgaa ccccagctcg acgctggcgg
     3961 tttttggcgg tagcgaagcc aacctgcgcg tcgggctgga aaccctgctc ggcgtgctca
     4021 atgccagcag tcgccagggc ttaaacgctg agttaacccg ctacacactc agcttgatgg
     4081 tgcttgagcg caaactctcc tcagcgaaag gcgcgctcga cactctgggc aaccggatca
     4141 acggcctgca acgccagctc gaacacttcg atttacagtc cgaaacgctg atgagcgcga
     4201 tggctgctat ctatgttgat gtgatcagcc cgcttggccc gcgcattcag gtcaccggtt
     4261 cccctgctgt actgcaaagc ccacaagtgc aggcgaaagt tcgcgcaacc ctgctggcag
     4321 gcattcgcgc cgccgtgctc tggcaccagg tcggcggcgg acgtctgcaa ctgatgtttt
     4381 ctcgtaatcg cctgaccact caggcaaaac aaattcttgc tcatttaacc ccggagttgt
     4441 gatctatgga attatcctca ctgaccgccg tttcccctgt cgatggacgc tacggcgata
     4501 aagtcagcgc gctgcgcggg attttcagcg aatatggttt gctgaaattc cgtgtacaag
     4561 ttgaagtacg ttggctgcaa aaactggccg cgcacgcagc gatcaaggaa gttcctgctt
     4621 ttgctgccga cgcaatcggt taccttgatg caattgtcgc caatttcagc gaagaagatg
     4681 ccgcacgcat caaaaccatc gagcgtacca ctaaccacga cgttaaagcg gttgagtatt
     4741 tcctgaaaga aaaagtggcg gagatcccgg aactgcacgc ggtttctgaa ttcatccact
     4801 ttgcctgtac ttcggaagat atcaataacc tctcccacgc attaatgctg aaaaccgcgc
     4861 gtgatgaagt gatcctgccg tactggcgtc aactgattga tggcattaaa gatctcgccg
     4921 ttcagtaccg cgatatcccg ctgctgtctc gtacccacgg tcagccagcc acgccgtcaa
     4981 ccatcggtaa agagatggca aacgtcgcct accgtatgga gcgccagtac cgccagctta
     5041 atcaggtgga gatcctcggc aaaatcaacg gcgcggtcgg taactataac gcccatatcg
     5101 ccgcttaccc ggaagttgac tggcatcagt tcagcgaaga gttcgtcacc tcgctgggta
     5161 ttcagtggaa cccgtacacc acccagatcg aaccgcacga ctacattgcc gaattgtttg
     5221 attgcgttgc gcgcttcaac accattctga tcgactttga ccgtgacgtc tggggttata
     5281 tcgcccttaa ccacttcaaa cagaaaacca ttgctggtga gattggttct tccaccatgc
     5341 cgcataaagt taacccgatt gacttcgaaa actccgaagg gaatctgggc ctttccaacg
     5401 cggtattgca gcatctggca agcaaactgc cggtttcccg ctggcagcgt gacctgaccg
     5461 actccaccgt gctgcgtaac ctcggcgtgg gtatcggtta tgcgctgatt gcgtatcaat
     5521 ccaccctgaa aggcgtgagc aaactggaag taaaccgtga ccatctgctg gatgagctgg
     5581 atcacaactg ggaagtgctg gctgagccaa tccagacagt tatgcgtcgc tatggcatcg
     5641 aaaaaccgta cgagaagctg aaagagctga ctcgcggtaa gcgcgttgac gccgaaggca
     5701 tgaagcagtt tatcgatggt ctggcgttgc cagaagaaga gaaagcccgc ctgaaagcga
     5761 tgacgccggc taactatatt ggtcgagcta tcaccatggt tgatgagctg aaataaactc
     5821 gtatcagtgc cggatggcga tgctgtccgg cctgtttatt aaaattatcc actttttctt
     5881 ttttcacttt acctcccctc cccgctggtt tatttaatgt ttacccccat aaacacataa
     5941 tcgcgttaca ctattttaat aattaagaca gggagaaata aaaatgcgcg tactggttgt
     6001 tgaagacaat gcgttgttac gtcaccacct taaagttcag attcaggatg ctggtcatca
     6061 ggtcgatgac gcagaagatg ccaaagaagc cgattattat ctcaatgaac atttaccgga
     6121 tattgcgatt gtcgatctcg gattgcctga cgaggacggt ctgtcactga ttcgccgctg
     6181 gcgtagcaat gatgtttcac tgccgattct ggtattaacc gcccgtgaaa gctggcagga
     6241 caaagtcgaa gtattaagtg ccggtgctga tgattacgtg actaaaccgt ttcatattga
     6301 agaggtgatg gcgcgaatgc aggcattaat gcggcgtaat agcggcctgg cttcacaggt
     6361 catttcgcta cccccgtttc aggttgatct ctctcgccgt gaattatcta ttaatgacga
     6421 agtgatcaaa ctgaccgcgt tcgaatacac cattatggaa acgttgatac gcaataatgg
     6481 caaagtggtg agcaaagatt cgttaatgct ccaactctat ccggatgcgg agctgcggga
     6541 aagccatacc attgatgtac tgatgggacg tctgcgcaaa aaaattcagg cacaatatcc
     6601 gcaagaagtg attactaccg ttcgcggcca gggctatttg ttcgaattgc gctgatgaaa
     6661 aaattactgc gtcttttttt cccgctctca ctgcgggtac gttttctgtt ggcaacggct
     6721 gcggtagtac tggtgctttc gcttgcctac ggaatggtcg cgctgatcgg ttatagcgtc
     6781 agtttcgata aaactacgtt tcggctgtta cgtggcgaga gcaatctgtt ctataccctt
     6841 gcgaagtggg aaaacaataa gttgcatgtc gagttacccg aaaatatcga caagcaaagc
     6901 cccaccatga cgctaattta tgatgagaac gggcagcttt tatgggcgca acgtgacgtg
     6961 ccctggctga tgaagatgat ccagcctgac tggctgaaat cgaatggttt tcatgaaatt
     7021 gaagcggatg ttaacgatac cagcctcttg ctgagtggag atcattcgat acagcaacag
     7081 ttgcaggaag tgcgggaaga tgatgacgac gcggagatga cccactcggt ggcagtaaac
     7141 gtctacccgg caacatcgcg gatgccaaaa ttaaccattg tggtggtgga taccattccg
     7201 gtggagctaa aaagttccta tatggtctgg agctggttta tctatgtgct ctcagccaat
     7261 ctgctgttag tgatcccgct gctgtgggtc gccgcctggt ggagtttacg ccccatcgaa
     7321 gccctggcaa aagaagtccg cgaactggaa gaacataacc gcgaattgct caatccagcc
     7381 acaacgcgag aactgaccag tctggtacga aacctgaacc gattgttaaa aagtgaacgc
     7441 gaacgttacg acaaataccg tacgacgctc accgacctga cccatagtct gaaaacgcca
     7501 ctggcggtgc tgcaaagtac gctgcgttct ctgcgtagtg aaaagatgag cgtcagtgat
     7561 gctgagccgg taatgctgga gcaaatcagc cgcatttcac agcaaattgg ctactacctg
     7621 catcgtgcca gtatgcgcgg cgggacattg ctcagccgcg agctgcatcc ggtcgcccca
     7681 ctgctggaca atctcacctc agcgctgaac aaagtgtatc aacgcaaagg ggtcaatatc
     7741 tctctcgata tttcgccaga gatcagcttt gtcggtgagc agaacgattt tgtcgaggtg
     7801 atgggcaacg tgctggataa tgcctgtaaa tattgcctcg agtttgtcga aatttctgca
     7861 aggcaaaccg acgagcatct ctatattgtg gtcgaggatg atggccccgg tattccatta
     7921 agcaagcgag aggtcatttt cgaccgtggt caacgggttg atactttacg ccctgggcaa
     7981 ggtgtagggc tggcggtagc ccgcgaaatc accgagcaat atgagggtaa aatcgtcgcc
     8041 ggagagagca tgctgggcgg tgcgcggatg gaggtgattt ttggtcgcca gcattctgcg
     8101 ccgaaagatg aataaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg
     8161 gttgcagata ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc
     8221 gattttcttg aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat
     8281 tttattgacc cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac
     8341 agtcgactgg tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc
     8401 tacgatcatc tcggtgaaac caactggtca ttactggtac aggcagtgaa ccactggcat
     8461 gagccgaccg ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat
     8521 ctgatgatct ctttttctgt accgggcggc ggcgtcggcc cgcatctcga tcagtacgac
     8581 gtgtttatca ttcagggtac cggtcgtcgt cgctggcgag tgggcgaaaa gctgcaaatg
     8641 aaacagcact gtccgcaccc ggatctgtta caggtcgatc cgttcgaagc catcatcgat
     8701 gaagagctgg agcctggcga tattctttat attccgccag gattcccgca tgaaggctac
     8761 gcgctggaaa atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta
     8821 attagcggat ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat
     8881 ccggatgttc cacctcgcgc tcatcctgcg gacgttctgc cgcaagagat ggataaactg
     8941 cgtgagatga tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag
     9001 tttatatccc agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca
     9061 gatgaaatct acgatgcgct gaaacaaggt gaagtgctgg tgcgcctggg tggtctgcgt
     9121 gtattgcgca ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt
     9181 ccggcactgg atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg
     9241 ctggaagatc cgtcattcct cgcaatgctc gcggcgctgg taaatagcgg gtattggttc
     9301 ttcgaagggt aagtttggat ttaagccgga tgtggtgctg attgcgcctt atcgtgccta
     9361 catgcactga acgcctgtag gcacgataag tatgtggacc agtgtcaata taacggtttc
     9421 atcccggtaa ttggcagtcc acatacgcat cgggcaatca cgacttccgt tgcgccgtta
     9481 actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga gtcacaaact
     9541 catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcaaggt aatcccataa
     9601 acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt cagttccggt tcaatatcgc
     9661 agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc actttctcgc
     9721 gcatattgta gtaactgtct tcaatcacca attcaatgta gcaatcagga tgtaaccctt
     9781 tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac tgtttacggt
     9841 cgaaatcacg gatgatgtaa tgcatatcgg cccgttcaac ggtgcctttc atgctcgcca
     9901 ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc ggaacttccg
     9961 catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc gtgcccgggt
    10021 gaacattgtt accgacaatt ttgatattga ccgacgcggc gttgaagttt tcaaactcca
    10081 gttcgcctac gccaccacca tcaacagtgt aagcccagcg ggcatcgaag gcgtcaacat
    10141 caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg cgaatatcac
    10201 catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct gcaatacctg
    10261 ctttgtcatc ggcacctaac aaggttttac catcggtagt aatcagcgtc tgacccagta
    10321 gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata cccagcgcaa
    10381 tatcgccacc gcgatagttt tcaacaattt gcggattcac atttttgccg ctgcaatccg
    10441 gtgaggtatc cacatgagaa ataaagccaa tcgccgggat attgccaggg acgttagccg
    10501 gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc cccatctctt
    10561 cgagctgctc tttcagtaga tgcaataact tccattggcc ttccgtgctg ggaacctgtc
    10621 tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc tcaagtagtt
    10681 tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag aaaagacaaa
    10741 tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat taccttaatg
    10801 aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg tttgccgtag
    10861 aattaccgcc ctcattaaga gtcaccaagg tggttaacca caaaccccgc atcggtaagc
    10921 catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga gttcgctttc
    10981 accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg tcattcccca
    11041 gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt ctggctgcgg
    11101 taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg gacgcatcat
    11161 gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga acactgtttt
    11221 ccaaagctac gcacttttcc cccacatgac cgtgttcgaa aatgtggcct ttgggttgcg
    11281 catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc tgcggatggt
    11341 gcaactggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc aacagcaacg
    11401 cgtcgccatt gctcgcgcgg tggttaacaa gcctcgtctg ttgttgctgg atgagtcgct
    11461 ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag cgttacagcg
    11521 taagcttggt attaccttcg tctttgtcac tcacgatcag gaagaagcgc tcaccatgtc
    11581 agacaggatt gtggtgatgc gcgagggtcg tattgagcaa gatggtacac cgcgcgaaat
    11641 ttacgaagag ccgaaaaacc tgtttgttgc cggcttcatt ggcgaaatca atatgtttaa
    11701 cgccactgtc atcgaacgtc tagacgagca gcgcgtacgc gccaacgttg aaggccgcga
    11761 atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg ttctgctgcg
    11821 cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag ggctgattgg
    11881 ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg aactggaaaa
    11941 tggcaagatg gtgatggtca gcgaattctt caatgaagac gaccctgact ttgaccactc
    12001 tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac tggctgatga
    12061 agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg cttgtgttgt
    12121 ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc gacgacgcca
    12181 gtttcgtcaa aatggtcttt acgctggata actacacgcg tctgctcgat ccgctctatt
    12241 ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc tgcctggtgc
    12301 tgggctaccc gtttgcctgg tttctggcga agctgccaca caaggtgcgt ccgctgctgc
    12361 tgtttctgct gattgttccc ttctggacca actcattgat tcgtatctac gggctgaaaa
    12421 ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc gttatcgaca
    12481 caccaatccg tatcatgttc acgcccagtg cggtgattat cggtctggtt tacattctgc
    12541 tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag cctttgcttg
    12601 aggcggcgcg cgatctcggt gccagcaagt tgcagacctt tatccgcatc attatcccac
    12661 tgacgatgcc ggggattatt gccggatgtc tgctggtgat gctgccagcg atgggcctgt
    12721 tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac gtcatcaaag
    12781 tacagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt acgctgacta
    12841 tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg aataagaagg
    12901 tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta tctacgcgta
    12961 cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc gctttggcat
    13021 caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg acagcctgtt
    13081 acaggcagca cagcattcac taacaatggc ggtgttttcg gcgacgtttg ctacgcttat
    13141 cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc cgttcgttag
    13201 cggaatgctg tttgtggtga tgatgtcgcc agatattgtg atggcgattt ctctgctggt
    13261 gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct cgcatatcac
    13321 cttctgcctg ccgtttgtgg tggtgacggt gtattcgcgc ctgaaaggtt ttgacgtgcg
    13381 gatgctggaa gcggcgaaag atctcggtgc cagtgaattt accattctgc gaaaaatcat
    13441 tctgccactg gcaatgcctg cggtggcggc gggctgggtg ttaagcttta ccctgtcgat
    13501 ggacgatgta gtagtttctt cgttcgtcac cggaccaagc tatgaaattc tgccgttaaa
    13561 aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg caaccatatt
    13621 actggtgctg tcgctggtga tggtaattgc cagccagctt attgctcgtg ataaaacgaa
    13681 aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc ggcgggtgct
    13741 ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca atacgctgta tttctacaac
    13801 tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac cggtattaag
    13861 gttatctatt cgacttacga gtcgaacgaa accatgtacg ccaagttgaa aacgtacaaa
    13921 gacggtgcct acgacctggt ggttccttca acctattacg tcgataaaat gcgtaaagaa
    13981 gggatgatcc agaagatcga caagtcgaag ttaagcaatt tcagcaacct cgatccagac
    14041 atgctcaaca agccgtttga cccgaataac gactactcca ttccgtatat ctggggggcg
    14101 acggcgattg gcgttaacgg tgatgcggta gatccgaaat ccgttaccag ctgggccgat
    14161 ctgtggaagc ctgagtacaa aggtagcctg ctgttgaccg acgatgcccg tgaagtgttc
    14221 cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa agagattgaa
    14281 gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa ctccgataac
    14341 ccggctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa cggttctgct
    14401 ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga aggcggcatt
    14461 ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg cgcgctgaaa
    14521 ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgccgaaac tatcggttat
    14581 ccaacgccaa accttgcggc gcgtaagctg ttaagcccgg aagtggcgaa cgataaaacg
    14641 ctctacccgg atgctgaaac cattaaaaat ggtgaatggc agaatgacgt tggcgcagcc
    14701 agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt cagcaagcgg
    14761 gcggtggcat taccgcccgt tttttagtta gcaaggatgt ggctaatgag tcaggacagt
    14821 aaagtgttat cccgcgcttt tctcggtatc ggtctgatat tgctcgttat ttctgcaatc
    14881 atcttttatt accattttac ttttaccaaa agcgcggttc atacagaagg aatcattgtt
    14941 gatgccgttt ggtacaacaa tcactcaaac gacgtcgatg ataatggttc ctggtatcca
    15001 gtggttgcct ttcgccctac gccagattac acgctaattt tcaattcgaa tatcggtagt
    15061 gatttttatg aagatagcga aggtgacaga gtcaatgtct attatccgcc agggcatcca
    15121 gaacaagccg aaataaacaa tccctgggtt aattttttta aatggggctt tgttggcata
    15181 gcaggcatta ttttcggtag tgtcggtctg ataatcacgc tcccctacac caaaaaatct
    15241 cgcaggaaac ggaagtcctg cccatgaaaa aattcattat actgctcagt ttactgatac
    15301 tcctgcctct ggtagcaacc agtaaaccat tgattccaat aatgaagaca ctttttacgg
    15361 atgttaccgg tactgtaccg gatgctgaag agatcgcacg taaagccgag ctttttcgcc
    15421 aacaaacggg aattgcaccg tttattgtga ttttacccga cattaataat gaagccagtc
    15481 tcagacaaaa tggtaaagcg atgctggcgc atgcgtcatc ttcattgagt aatgtaaaag
    15541 gaagtgttct gttactattt actacccgcg aaccacggtt aattatgatc accaacggcc
    15601 aggttgaaag tggtctggac gataaacatc tcggccttct gatagaaaat cacacgctgg
    15661 cttatttaaa tgcagatctc tggtatcagg gaatcaataa tgcattggct gttctacaag
    15721 cacagatatt aaaacaaccg acgccgccac tgacgtatta tccgcatcca ggacaacagc
    15781 atgaaaatgc tccccccggc agcaccaaca cattaggttt tatcgcctgg gctgcgacgt
    15841 ttatcttatt ttcctggatc ttttgctaca cgactcgttt tatttatgcg ttaaaatttg
    15901 cagtggcgat gactattgcc aatatgggtt atcaggcttt atgtttgtat atcgataata
    15961 gctttgcgat taccagaata tcgccactct gggcagggct aattggtgtc tgtacgttta
    16021 ttgccgcatt gttattgacg agtaaacgat aaaagaggcc ggaaaagtgc gcgtgtatta
    16081 tgccggatgg ggcgcgaacg ccttatccgg cccacaaaac ccgcaaattc aattaattgc
    16141 gtccccttgt aggcctgata agcgtagcgc atcaggcaat gcttcctgct ttataatccc
    16201 ttcagcaact tttcaacaaa ctcaggcacc acctggcttg ccgggccgta atatttctcg
    16261 gcaaattcat taccgacctg acttggttcg agattcagtt ccacggtgtg cgcgccatgc
    16321 agtttcgctt cgtgaacaaa cccagccgcc ggataaacat gcccggaagt accaatggca
    16381 atgaaaatat cagccatcga caacgccata taaatttcat ccatgccgag tggcatttcg
    16441 ccaaaccaaa cgacgtgcgg gcgcaagggt gccgggaact ggcaacaatg gcatttatct
    16501 tctggcgtaa cgtctccggt ccagtcgaga acctgaccac tttgcgaaca acgaactttc
    16561 agcaattccc catgcatatg aatgacattg gtattacctg cgcgttcatg caggttgtct
    16621 atattctgcg ttaccagcaa aaagcgatcg ccgagggcgt cttgcagttt agccagcgca
    16681 agatgcgcgg cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa
    16741 aacgcttgca ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc
    16801 cgatgttctt cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa
    16861 atccctgccc ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta
    16921 tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc ggcgtttatt tttacgaaaa
    16981 cgacttaacc gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga
    17041 tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgc
    17101 ggaacacgag ctacaggtaa gagatgacga ggcagcctgt ccgccagttg cgttgtgatt
    17161 gccgggaaat tcgataagcc accaccaatg acgaccaggt cagggtcaac aatggtcagg
    17221 atatttccca gacaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc
    17281 tcatcgcctt gatcataaag cgcaataatt tcgggagcct gcaacggttg atgataatag
    17341 tgttgataca gccacgcaaa accgcgacca gacagataat tttcaatgca gccatgctga
    17401 ccacagccgc agcggcgtaa cgggaaatcc aaccccatca tggttaacgc atcaaccggc
    17461 agacgcatat ggccaaactc gccggtaatg tagcttttac cggtaatcgg tttgccgttg
    17521 aaaatcagcc cgccgccaac gccggtgccg agaatcaacc ccatcaccag tggatattga
    17581 gtaaattcat catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt
    17641 acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg gtttaccgct ggcagcgggg
    17701 acattggcgg catacagcgt accatcttct gtttccggca ttcccggaat accgatgccg
    17761 acagaacctt tacagccaaa acgttggtca gcttcagcta ccagctcaca cactgcatct
    17821 aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga
    17881 ccgctatcaa acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt
    17941 gccgctcctt gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat
    18001 attactggcg cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac
    18061 cagtacgtag aagacgtcca gccagtgcaa ttccgatggc aggaaatcaa taaaatagat
    18121 atcgctggag aggaactgat gaccgatcaa cttttcaatc cactcaataa tcggggtaag
    18181 ttgcagtgaa accaccacac cgataatcac cccacacagg ctgccgaaca gccctgccag
    18241 caatccatac cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa
    18301 tactgcgata tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa
    18361 acaggccacg ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatctggat
    18421 atcgcgatac atatagccgt aagtaccaat ccaactttta atataaacat agctgttggt
    18481 cacttcaccc gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc
    18541 aatacctgac acgctggaac ccatatcaag atattgttgg gcatccgcca gcgggatcat
    18601 ggcaaaactg tgatcgagtt gaccactcaa ctgcaaaata ccggcaacgt gcaaacgcac
    18661 acgttttggc tgcatcagtt tatgctcggg attcgagttg gggatcataa tcgacaccca
    18721 atccccctgc ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc
    18781 gcccgcttta aaattgcgcc acgcatcgcc ctggacaaac gagggtaatg cgctcagacg
    18841 ctgttcctgt tgcgggttaa cgcccttcac ctggattgcg cgaagattca cgccactttc
    18901 caccagcccg gtgaaattga tatacggcgc ggcagcggca atacccggca ctttttgcac
    18961 gtgatccagt gcttcctgcc agttagtcca cggctgatcc accgcctcga tttcaccatg
    19021 cggcaccacc gccagaatac ggttgttcag ttcgcgttca aagccgttca tcgcgcttaa
    19081 gccgacgatc aataccgcca cgccgagggc aatgccaatg gtagaaataa cggagatcag
    19141 cgacaccatg ccgccgcgcc gccgtccgcg actaaaacgc aggccaatta ataacgataa
    19201 aggcatcgcc attactccgc ccccatcagg ctcagttccg ccgtcagacg accatcacgc
    19261 atttctaact ggcggctcat acgtttcgcc agttgcaggt cgtgagtaac caccaggaag
    19321 gcggtgccct gcaagcgatt caattcccca agcaactgga agatgctgtc ggcgttacgc
    19381 gcatcgaggt taccggtagg ttcatccgcc agtaccaggc gcgggttatt caccagcgca
    19441 cgggcaatcg ccacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca
    19501 cgatgatcca gccccaccgc ttttaacatc tcaagtgcac ggctgttgat ttcagcgggc
    19561 tttttcttgc caatcagcag cggcatagcc acgttttcca gggcggtaaa atccggcagc
    19621 aggtggtgaa actgataaat aaagcccagc ttctggttgc gcagctctgc tttcgccgcc
    19681 gaagacagtt tgctcatcgg ttgaccgtta aagatcacat cgccggaggt tggcgtatcc
    19741 agcccgccca acaagtgcag caaggtgctt ttaccggaac cagagctacc gacgatcgcc
    19801 atcatttcac cttcgccgac gctgaaactg acattgtgca gcacatcggt ttgcacactg
    19861 ccttcctgat agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa
    19921 agcctcagcg ggttgagtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc
    19981 gatagccatc gccaccagcg caataacaat gacctgtaaa ggttcgatag cgaccggcag
    20041 cgccgcgcca tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag
    20101 cagggcgcca agcgccgctc cgaggatcgc accgataatc ccggcgctgg ccccttgcac
    20161 cataaagacc atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgctacttc
    20221 gccctgcttc tccattacca tcagccctag tgaggtaata atgttaaacg ccgcaacggc
    20281 gacaatcagg ctcagcagta aacccatcat atttttttcc atgcgtacgg cctggaacaa
    20341 ctcgccttta cgatcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact
    20401 taatgagtcg actttcagcg gctcatccag ccacaaacgc cagccggtaa tattgcctgc
    20461 cggataacgc atcagacgcg aggcatcctc aatattcacc agcatttcat agccatcgac
    20521 ttcactgttg gcggcgaaag tgccaatcac attgaacagg cgctggcttg gaatacgccc
    20581 catcggcgtg aactggctgg cagatggtac catcacgcgg atttgatcac cgcgattaac
    20641 gcctagctgt gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc
    20701 agtttgtttc acattgacca gatacggtgt aagtggatct ttttgcgccg ggtcgatacc
    20761 gagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat
    20821 aggtgcgacg cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt
    20881 aagagagcca tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg
    20941 cagctcgcgc tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag
    21001 ggtaatgccg atggtagaaa gccaggagac gaaacgaccg aagcgatccg ctgcacgccc
    21061 acgcatgtaa cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt
    21121 gctgtagcaa agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa
    21181 gtgatcgtca gggctaatct gaaaagcccc cgtgaatcaa tcggctaaat tgccatgttt
    21241 tatcatcaat cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc
    21301 attacacata gggtcataac ccgcaggatc aaccgcatgt aaattaaaaa agagcgtgtt
    21361 gtcctgatga aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg
    21421 gtggtgattt atcactcggt cattaccttt tatccgcatc tgaccacttt tcagcatccg
    21481 ttatcggaag tcctgagcaa atgctggatc tatttcaatc tttaccttgc accctttcgt
    21541 atgccggttt ttttctttat ttccggctat ttgattcgcc gctatatcga cagcgtaccg
    21601 tggggaaatt gtctcgataa acgcatctgg agcatctttt gggtgctggc gctttggggc
    21661 gtggtgcagt ggctggcgtt aagtgcgctt aaccagtggc tggcacctga gcgcgattta
    21721 agcaacgctt ccaatgccgc gtatgccgat tccaccggtg agttcctgca cgggatgatc
    21781 acagccagca ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa
    21841 atttttagcc gtctggcgct gccactattc gccttgtttg tactgctgag tgtggcggtt
    21901 aatttcgttc ccacgccgtg gtggggaatg aacagtgtga tccgcaattt gccttattac
    21961 agccttggcg catggtttgg cgcaacatta atgacctgtg ttaaagaggt gccgttgcgc
    22021 cgccatctgc tgatggcttc tttgcttacc gttctggcgg tcggtgcctg gttgtttact
    22081 atctcgctgc tgttgtcgct ggtatcgatt gtggtgatca tgaagctgtt ttatcaatac
    22141 gagcaacaat tcggtatgcg ctccaccagc ctgctgaatg tgattggctc caacaccatt
    22201 gctatctaca ccacccatcg cattctggtt gaaatattca gcttaactct gcttgcgcaa
    22261 atgaacgcag cacgctggtc gccgcaagtc gaactgacac tcctgctggg ttaccccttt
    22321 gttagtttgt tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgcgca
    22381 ttcagcgatc tgttgttctc cccgccttct ctgcccgcgg ccatcagtta ctcccgctaa
    22441 gccatcgccc cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac
    22501 agcattgctt atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac
    22561 aaccgttatg cctgaacaat atcgttatac gctgcccgtc aaagcgggtg agcagcgtct
    22621 gctgggcgag ttaaccggcg ctgcctgcgc gacgctggta gcggaaattg ccgaacgtca
    22681 cgccggtccg gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt tgcatgatga
    22741 aatcagccag tttaccgatc aaatggtgat gaatctggcg gactgggaaa ctcttcccta
    22801 cgacagtttt tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct
    22861 accgacgatg cagcgtggcg tactgattgt tccggtgaat acgcttatgc agcgcgtttg
    22921 cccgcacagt tttcttcacg gtcatgcgct ggtgatgaaa aaaggtcagc gtctgtcacg
    22981 agatgcatta agaacccaac tggacagcgc cggttatcgc catgttgacc aggtaatgga
    23041 gcacggcgaa tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagct
    23101 gccttatcgt cttgatttct ttgatgatga aatcgacagc ctgcgggtgt ttgacgtcga
    23161 cagccagcgc acgctggagg aagtagaagc gatcaatctg ctgcccgcgc acgaatttcc
    23221 gaccgataaa gcggcaattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa
    23281 gcgcgatccg gaacatattt accagcaagt gagtaaaggc acattacctg ccgggatcga
    23341 gtactggcag ccattgttct tcagcgaacc actgccgccg ctgttcagtt atttccctgc
    23401 caataccttg ctggtgaata ctggcgatct ggaaaccagt gccgaacgtt tccaggctga
    23461 cacgctggcg cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt tgccaccaca
    23521 atcgctctgg ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgggtgca
    23581 gctaaaaact gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact
    23641 gccagacctg gccgttcagg cacaacaaaa agcgccgctg gatgcgctgc gtaagttcct
    23701 cgagactttc gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc gtgaagcgct
    23761 gggtgaactg ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc
    23821 cagcgaccgt gggcgttatc tgatgattgg cgctgccgaa catggttttg tcgataaggt
    23881 gcgtaatctg gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg cccgccgtcg
    23941 tcaggattct cgccgcacca tcaaccccga tacactgatc cgtaaccttg cggagctgca
    24001 tattggtcag ccggtggtcc atctggagca cggtgtcggg cgctatgccg gaatgaccac
    24061 gctcgaagcg ggcggcatta ctggcgagta tttgatgctc acctatgcca acgacgccaa
    24121 actgtatgtt ccggtgtcgt cactgcatct gattagccgt tacgcaggtg gcgcggaaga
    24181 aaacgccccg ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc
    24241 ggaaaaagtg cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa
    24301 agagggcttc gcgtttaaac acgatcgtga gcagtatcag ttgttctgcg acagcttccc
    24361 gtttgaaacc acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca
    24421 gccgctggca atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc
    24481 gatgcgcgcc gctttcctgg cagtagataa ccacaagcag gtggcggtgt tggtgcctac
    24541 cacccttctc gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt
    24601 acgtatcgaa atgatctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga
    24661 agtggcggaa gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt
    24721 caagtttaaa gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca
    24781 taaagagcgt attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc
    24841 gatcccacgt acgctgaata tggcaatgag cggaatgcgt gacctgtcga ttatcgccac
    24901 gccgcccgcc cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt
    24961 ccgggaggcg atcctgcgtg aaattttgcg cggaggacag gtttattatc tctacaatga
    25021 tgtggaaaac attcagaaag ccgccgaacg gctggcagaa ctggtgccag aagcgcggat
    25081 cgccatcggt cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca
    25141 tcatcaacgt ttcaacgtgc tggtttgtac aaccattatc gaaaccgggc tcgacatccc
    25201 gacagccaac accattatca ttgaacgcgc ggatcacttc ggtctggcgc agctgcacca
    25261 gttacgcggt cgcgtcggac gttcacatca tcaggcatat gcatggctgc tgacgccgca
    25321 tccaaaagcg atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga
    25381 tctcggtgca ggctttgcgc tggcaacgca cgatctggag atccgcggcg cgggtgaact
    25441 gcttggcgaa gaacaaagtg gctcaatgga aaccatcggt ttctcgctgt atatggagtt
    25501 gctggaaaac gccgtcgatg cactgaaatc cggacgcgag ccgtcgctgg aagatctcac
    25561 cagccagcaa acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcattcc
    25621 tgacgtgaat acgcgtttgt cgttctataa acgtattgcc agcgccaaaa cggaaaacga
    25681 actggaagag atcaaagtcg agcttatcga tcgcttcggc ctgctgccgg atccggcgcg
    25741 taccctgctg gatgttgccc gtctgcgcca gcaagcgcag aaactgggga tcaggaagct
    25801 ggaaggtaat gagaaaggcg gggtgatcga atttgccgag aagaatcacg ttaatccggc
    25861 ctggttgatt ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg
    25921 cctgaaattt attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt
    25981 tatgcgtgaa ctggaagaga acgcgatcgc ttaatggtaa atcgggggcg tttctgcgcc
    26041 cccatctgca acatttacaa attctttgca cttccctgca ctatccgaca ctgtcaccat
    26101 ccataattca ggcttatctg tttattacaa taaccttata tttattatgg atttttggtg
    26161 atgatcaaaa cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg
    26221 ctggcgctac cggcaaaagc caacacctgg ccgctgccgc cagcgggcag tcgtctggtt
    26281 ggcgaaaaca aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa
    26341 aaatataacg tcggctttct cgctctgtta caggctaacc ctggcgttga tccttacgta
    26401 ccgcgcgcgg gtagcgtgtt aacgatcccg ttgcaaaccc tacttccaga tgcgccgcgc
    26461 gaaggcattg tgatcaatat tgcggagctg cgtctctatt actacccgcc gggtaaaaat
    26521 tcggtaaccg tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca
    26581 atggtgacca ccgtttcaga caaacgtgca aacccaacct ggacgccaac ggcaaacatc
    26641 cgcgcccgtt ataaagcaca gggaattgag ttgcctgcgg tagtgccggc tggaccggat
    26701 aacccaatgg gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac
    26761 ggtacgaacg ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg
    26821 gatgacgata tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc
    26881 aacactccga taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag
    26941 ccgctgtcag agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca
    27001 atgcaatcat ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat
    27061 gtccgttccg ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aactctgtaa
    27121 atcctggata aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc
    27181 aggcattaat gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg
    27241 gaccggttac agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca
    27301 gctgctcttc cagcgatccc agatttgtcc ccgcgttagc actgattgta ccgacttttt
    27361 gttggccttc tggcgttgac tgaacttcga cagccgcaaa gctggcaaat gacatggagc
    27421 ttaaaatcgc cgcagcgatg agggttttta cgtttttcat aatagtggcc ttatgcagat
    27481 gaatgacgtc gcaagcaatt acttaacgat cgataactaa atgatagatg tgatctggat
    27541 cacatacaag atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt
    27601 taaatagaaa gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta
    27661 taataacctt tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca
    27721 atgtgtaaaa aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa
    27781 ggccatgaaa ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga
    27841 agcgaccgga gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt
    27901 tcgtgccgtt ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt
    27961 ttgtgaagag aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg
    28021 ctttaccagc aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc
    28081 cccagattca ggagatatcg ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac
    28141 tttgcagcag tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt
    28201 gacacatctg gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga
    28261 aggtgcatcg ctggaaaaac taatgcagat tgccggaacg actttgcgtt tatggcccga
    28321 actggtgaaa taagtcgcaa aaaaagcccc tcagcctgag cgaatgaggg gccaaattgc
    28381 agagtacagc cttgttatac tttgttattc aaaatcagtt gcccattact atctagcggg
    28441 atctgcgtac ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag
    28501 gtcacatcat aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc
    28561 gtagtcgtgt aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca
    28621 ccacccagcg cccccacaac agtggcgaca tctttaccgc gaccaccgcc aaactgatgc
    28681 cctatcacgc cgccagcaac agcgccgagc accgacccgg taatgcgatt ttcatcctgc
    28741 accggtcgac gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt
    28801 tccttgattg gggttgcaga aacaacctga gcgtattgcg ggccacgttc aaacacgttc
    28861 agactggcca ctgccgctac gcccagcgca gctgcgacac caatcccgat acccgccaac
    28921 attgatttat tcacgggaac acctccttct ttgcagttat gccaatcatg caagcgaagg
    28981 agaaaagcag ctatcagaca gaagatgaaa agcgcgggaa tggctggaaa tacttaagga
    29041 ttcggaggag tctgaaggaa tgcctgatgc gacgcggctg ccgatcgcgc tgttcctcaa
    29101 gggcggataa ggcgtttatg ccacatccgc cagtgtacgt cgattaatgc aacttcaaac
    29161 gcggacggat aacgcggtta atactcccca ccagcatcat taatccggtt ttaaagtaac
    29221 catgcagcgc aatctgatgc attcggtata gcgagatata tacaaagcgc gcaattcgtc
    29281 cttcaatcat cattgagccg cgcgtcaggt tacccatcag gctaccaacg gtggagaagt
    29341 tcgacagcga taccagcgaa ccatgatctt tatactgata atttttcagc ggcttaccat
    29401 tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat ctggtgtgca gcctgagcac
    29461 gcggcggaac aaagccccct tccggacgcg ggcatgacgc gcagtcgcca atagcgtaaa
    29521 tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac cagctggttg atacggttag
    29581 tttcaagacc accgatatct ttcaggaagt ctggcgcttt gatcccggct gcccacacca
    29641 tcagatcagc ctcaatatat tcaccatctt tagtgtgcag gccgccttca tcagcactgg
    29701 tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt tagctcgttg tgggccgcag
    29761 cagagatacg tggcggtaat gcaggcaaaa tacgttctcc cgcttctacc agcgttacgt
    29821 tcagggcttc gttggtcagg cctttgtaac cgtagctgtg cagttgcttg accgcgttgt
    29881 gcaattcagc ggagagttct acacccgtcg cgccgccgcc gacaatcgca atgttcactt
    29941 tgccatttgc gcccaggttg gcggagtatt tcaggaagag attcagcatc tcctggtgaa
    30001 agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt ctctttgaca cctggcgtat
    30061 tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt gtcataggcg attttacgtt
    30121 ccggaaccag cagttcacct ttctcatcgc gcagttctgc aatagtgatt gttttcgctt
    30181 cacgatcaat atcaatgacg gaacccagct ggaactggaa accatgattg cgcgcatggg
    30241 ccagatagct caacgcatcg acgccttcat caagcgagcc agtcgccact tcgtgcagca
    30301 gcggtttcca caggtggctg tggttacgat cgaccagcgt aattttggct tttttcttgc
    30361 gtcccagctt atgccccagc tgtgttgcca tttccagccc accagcaccg ccgccgacaa
    30421 tcacaatctt tttcaatggc gtagtcaacg tgaccccctt aaatttatta accaattgtt
    30481 aattaatggt tattaacata gctcttaatt aacaacaggt tacagcaatg aaaatgttct
    30541 tcgctattga gaataacatg aatggtgcat tggtcatacc aaaattgatg ttaatcaagt
    30601 tttgttgctg aaaagatagg cgactttacc gggtgagatc aaaaaacaga gtttattgtt
    30661 tattatttga gcaagtccag aagggcgata tagggtgaag cgtatatcga gggcgtttgg
    30721 tcgtcctgat aagaggcgca tcaggcattg atgctcgaat gtcggatgcg gcgtgaacgc
    30781 cttatccgac ccacagaatc cggagcgcat caggtaccgg caaggttacc tgatgcgacg
    30841 agggcattta cccgagggtt ttgaacgctt taatgcgctg taaatgcggg gagatattct
    30901 tgaatttgtg cgtctgctct tcgtcccaga caatctcgta ataatgatgc agctcttcag
    30961 atgtccgctg gctgttaagc gcttcatcat tacgcgacaa aatcaccaga caacgatcgc
    31021 gattcttctc gcggaagttg gtcacacact tagtggcaat atcggcatac tcttccgggc
    31081 gatcaatctt gccttccatg ttctcataag ggaacaaatt agggttgaag atcacctggc
    31141 ggatgtcgca aagaaaacca atccgttccg cccagtatcc gcccaggcca acgccgcaaa
    31201 ttagcggacg ctcgtcaacg ttcagttgca acattttgtc cacttcttta agcagatgct
    31261 gcatatcatg tttcggatgc cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa
    31321 actgcaattg taagactttc tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat
    31381 agataatcat cgcttcctca cataagccag ggttagtgac ctgctttctc ttcctgccag
    31441 cgttcgtgta actgattcag acgggtgctg atcgctttcc agcgagccga gtccatcagt
    31501 tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa cacgttctgc cttgatcggt
    31561 gacagattat ctaacacgct gacggcccct ttacgattat tgcagaccag gatcatatcg
    31621 caacccgcat ccagtgatgc ctgcccgcgt tcggcataac tgcccataat cgcggcacct
    31681 tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac ccagttcctg acgcaaaacg
    31741 gttttcagcc agtagggaga accgctcgcc ggacgcggat caacatcact gtagatcaca
    31801 tgcgcaggca taatggcgtc gagtttattt tcgcggatta aggaactgaa gaccgacata
    31861 tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg gtgtttcttt gtgtgagtct
    31921 gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc
    31981 ataccatcaa taaaccggct ggcaattgcc agggcttttt gtggatcggc atgataagaa
    32041 cgctcgccaa tcgccgcgct gatatgcccg acatccagca ctggcgcaaa gctgatatca
    32101 atatccatag cgatcatttc gctggccatc agccaacccg cctcttgcgc cagtttgccg
    32161 ccctcttcca ttcctgacag cgcagcgaat gattgcgccg ctggcaagcg ggtaaaacct
    32221 tcacgaaaac gctgcacgcg tccaccttcc tgatctaccg ccaccaccag atgattgcgc
    32281 gaagctgcgc ggatctggcg caccagttca cgtaactggg caggatcatg atagttacgc
    32341 gtaaagagaa tcagccctcc caccagcgga tgcgcaagta tttcacgctc ttccgcctcc
    32401 agttcgtaac ctttgacatc caacattact ggacccacac tgctctcctt attgttttat
    32461 taatagctgc cgccaggtgt catcggccag cctgataaat tgttgatcgc cggtttgtcg
    32521 ccagcggtac tcaaaccacc ctgctttgag catcagcagc cagggaaacc atcgcctgac
    32581 ctgacgccat aattgcgccg gataaatctt cgcgcgagtg gcatagtcat tgaccaattg
    32641 ccggtgctgt tcagtatttt ccacccacac cgccgccagt tccagcgcga tatcaccatc
    32701 tccggcatac tcccagtcga tgagttttaa ccctgacgcg ctatgcacta aatttccggc
    32761 gtggacatcc atatgcaatg gacttaagcg taaaggccgt ggttcccgca ctttgcgcag
    32821 acgttttaac attcgcagcc aacccactgt ccgccgcgcc ggatcgcttt gctgccagta
    32881 cagttccagt aacggcaaca gcgttattcg ccagccaaaa cgtggttgtt gatgtagata
    32941 atacagcaag cctgccagtt cgttggtatc cggcaaatac gtttttaccg cgccgggcag
    33001 atagtcgact accatccagt cacggagata taaatgcggc ttcggtgcaa tgcttgcggg
    33061 tagttgtgat aaagcccgat actggcgcaa gaacgcggac tgcggcgcat caggatcgtg
    33121 cggctgacgc acaacaaaac gctggccctg atgttcaatg agaaaactcc cgccactaag
    33181 cccactatga aacgtcgtga cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc
    33241 gcgcatgatg ggattattgc tgcgaaacgg cacctttacc tgaccagata atttcgcccg
    33301 tctgcaccag catcagctgc atttgtaggg tcggagcgtt aacgttgcca gaggcgctgg
    33361 agtacagcac gtaatgagcg ccgacattgc gggcaatgcc tatggcttta ctacgagtac
    33421 ctaaactgtc ctgcggcgac aaacctaact gttgcttagc catcgacagc tgctgggcgg
    33481 aaaccagggt aaatttcccg ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg
    33541 cggcattcag cgaaccgtta gtacggttgt taacgctatc aaccagcagg acgctacccg
    33601 cagtcacccc gtcagcccca agcatcttac tgaccatcgg ctgcattgcg ccattccagt
    33661 cataatggcg aatatgcggc gcaggcggtg ccgtttgatc ttcgtgctca attgggcctg
    33721 gctgctgcgg gatcgtcggc accgagggca ctgtggggac aggctgttgt ggctcggctg
    33781 gttgttccgg cgctggtttc acttcttcta ccggtgcagg ttcacgttgc cccacacatc
    33841 cggcgagaaa catcgccagc gcggtaatca aggcgtagcg actcattttt gtcatcaaga
    33901 ttcacccctt acaaatatag ataaagtctg actttgtgcg cccccagaaa attggcgctg
    33961 ccgtacagcg ttaccgccga atgtgcggga atggtaacgc tgcgtggcct ttccagagga
    34021 tgcatctcca gccctctggc gtcataccag taaaaacgat aatgaacggt aacgggttct
    34081 tgcctttcgt tataaagcgt tgaggatgct gaaggttgaa tatcagacgt cgaaaggacg
    34141 ggcttttctg cactgatgcc cgcagccaat aaagatgact ccatcaccag cgattgctca
    34201 tcattcaccg gaatttccgg atgtgaacga cagcccacca gcagtaacaa cgccagagac
    34261 accagcccaa agcatccttt tctcatcgtt acagaccttt atgcgccagc attggtccca
    34321 gcggacggcc acccaacaag tgcatatgga tgtggtaaac ctcttgtccg ccatggcggt
    34381 tggtattcat gatcagacga tagccatctt cggcaatacc ttcttgctca gcaatttttg
    34441 ccgctacggt gatcatgcgt cccagcgcct gctcatgctc agctgagacg tcgttcacag
    34501 tcggaatgag gatattcgga atgatcagaa tatgcgttgg cgcttgcggc gaaatatcgc
    34561 gaaacgccgt taccagatca tcctggtaga cgatatcgga ggggatctca cgacgaataa
    34621 ttttgctgaa tatagtttct tctgccacga cattttcctt tttcaaatag ccatgcgttg
    34681 cgccatgcta cgccgcgcca cactgcgagt atagagtatg gtcgagttaa acctgctctt
    34741 tcaaccttaa ctcacgattt cttaagcaaa aaatcgtttt gctgccgtat atatcgccat
    34801 tattcccatt tctgcctgcg aatgcgctga aaagacattc tctgtttggt ttacacgaca
    34861 aatatgaatg cgtatatttc tcatttgcat ttacaaacaa aattattcgc acactaaata
    34921 taatcgctca tcaccctcct ccggcatgag cctgacgaca acataaacca agagatttca
    34981 aatgctttca acacaattta acagggataa tcaatatcaa gccatcacca aaccgtcact
    35041 acttgccggt tgcatagcac tggcactatt accttctgcc gcttttgctg caccagccac
    35101 tgaagaaacg gtgattgttg agggttcagc cacagctcca gatgatggcg aaaatgatta
    35161 cagcgtaacg tctacctctg cgggtaccaa aatgcagatg actcaacgtg atattcctca
    35221 gtcggtcact attgttagcc agcagcggat ggaagatcag cagttacaaa cgctgggcga
    35281 agtgatggaa aacacgcagg ggatcagcaa aagtcaggcg gattccgatc gtgctcttta
    35341 ttattcccgc ggattccaga tcgataacta tatggttgat ggtatcccca cctattttga
    35401 atcgcgctgg aatctgggcg acgcactttc tgatatggca ctctttgaac gcgtagaagt
    35461 agtgcgtggc gcgacaggac tcatgaccgg gacgggtaat ccatctgcgg caattaatat
    35521 ggttcgaaaa cacgcgacca gtcgtgaatt taaaggcgat gtctcggcgg aatacggtag
    35581 ctggaacaaa gaacggtatg tggcggattt acaaagccca ctcaccgaag acggtaaaat
    35641 ccgcgcgcga attgtcggcg gctaccagaa taacgactca tggctggacc gctacaacag
    35701 tgaaaagacc ttcttctccg gcattgtcga tgctgattta ggcgatctta cgacgctttc
    35761 agccggttac gaatatcagc gcattgatgt taatagccca acctggggcg gtttaccgcg
    35821 ctggaatact gatggcagca gcaacagtta cgatcgcgca cgcagtaccg cacctgactg
    35881 ggcgtacaac gataaagaga tcaacaaggt ctttatgacc ctgaagcagc ggtttgctga
    35941 tacctggcaa gcgacgctga atgccaccca ctctgaagtc gaatttgaca gcaaaatgat
    36001 gtatgtcgat gcctatgtaa acaaagcgga tggtatgctg gttgggccat acagtaatta
    36061 tggacctggc tttgattatg tcggcggcac cggttggaac agtggcaaac gtaaagttga
    36121 tgcgctggat ttgttcgctg acggtagtta tgaattgttt ggtcgtcagc acaatctaat
    36181 gtttggtggc agttacagca aacaaaacaa tcgttacttc agttcatggg ccaacatctt
    36241 cccggatgaa attggcagtt tctacaactt taatggcaat ttcccacaaa ccgactggtc
    36301 accacagagc ctggcgcagg acgataccac acatatgaaa tcgttatatg ctgccactcg
    36361 tgtcaccctt gccgatccgc tgcatctgat cctcggcgca cgttatacca actggcgggt
    36421 tgatacgctg acttacagca tggagaaaaa ccacaccacg ccttacgctg gtctggtgtt
    36481 tgacatcaat gacaactggt cgacctacgc cagctatacc tctattttcc agccgcaaaa
    36541 tgatcgtgac agttcaggca aatatctggc tccaatcacc ggtaacaact acgagctggg
    36601 tctgaaatcg gactggatga atagccgtct gaccaccacg ttagccatct tccgtattga
    36661 gcaggataat gtcgctcagt ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc
    36721 ctataaagca gtggatggga cagtcagtaa aggggtggaa tttgaactca acggcgcaat
    36781 taccgacaac tggcagctga catttggcgc aacgcgctat attgcagagg ataacgaagg
    36841 aaacgccgtt aatcctaatc tgccacgcac cacggttaaa atgttcacca gctatcggtt
    36901 gcctgtcatg ccagagttga cagtcggcgg tggtgttaac tggcaaaatc gcgtgtatac
    36961 cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa ggtagctacg cgctggtgga
    37021 tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta caggggaacg tcaataacct
    37081 gttcgacaaa acctacgata ccaacgtgga aggttctatc gtctacggcg caccgcgtaa
    37141 tttcagcatt accggcacgt atcaattctg aataacacct gtaaaaaagg cagccatctg
    37201 gctgccttag tctccccaac gtcttacaga ttagtggtta cggatgtact catccatctc
    37261 ggttttcagg ttgtcggatt tagtaccgaa aatcgcctga acaccagaac cagcaaccac
    37321 tacgcccgct gcgcccagtt tcttcaggcc agcctgatcc actttagaca catcagcaac
    37381 gctgacgcgc agacgggtaa tacatgcgtc gaggttagta atgttttctt taccaccaaa
    37441 tgcagcaacc agagccggtg ccatttcgct ggtacctgtc gcttttgcat cttcagtcgc
    37501 gtcttcacga cccggcgttt tcagatccag tgctttaatc agcacgcgga agatggtgta
    37561 gtaaacaatc gcataaccga taccgacgat cgggaacagc cacagtttgc tgctgttacc
    37621 agacagaacg atgaagtcga tcagaccgtg cgagaacgac gtaccgtcac gcatccccag
    37681 aagaatacag attgggaatg ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg
    37741 cgcaacgaac atgaaggaga actcgatcgg ctcggtgata ccggtcagga acgaggtcag
    37801 cgccgcggag atcataatac cgcccacttt cgcgcggttt tctggtttag cagagtgcca
    37861 gatagcaatt gcggcagctg gcagaccgta cattttgaac aggaagccac cagacagttt
    37921 acccgcagtc gggtcacccg ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc
    37981 tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg ttccagatgt ggtgcagacc
    38041 aaacggtacc aggcaacgtt cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg
    38101 gtaagcagcc cactgagaga aggtctggat tgcagaacca atcggcggcc aaatgaagga
    38161 cagcacaacg ccagtaaaga tggcagccag gccagaaatg atcggcacaa agcgtttacc
    38221 ggcaaagaag ccaagatact caggcagctt aatacggtag aaacggttaa acatgtacgc
    38281 tgcgatcgca ccggagataa tccctccgag tacgccagta tccgccaggt gtttagaggc
    38341 gatttcttca gcaggtaaat gcagtaccag tggcgcaacc acggccatgg ttttaaccat
    38401 gatgccatag gcaacaactg cggccagcgc ggatacgcca tcgttattgg taaagccgag
    38461 ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc
    38521 cataacatgc gatacaacgg cgggcagcca gctgaaattc gcggaaccga cgcccagcag
    38581 aatacctgcg ataggcagta cggataccgg cagcatcagc gatttaccga ccttttgcag
    38641 gttagcaaat gcattcttaa acataattga gagtgctcct gagtatgggt gcttttttta
    38701 cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta cagggcatct aagcgccctt
    38761 tatttattac acagagtaaa ataattcagt gccaatttgt ttgacggcta tcacgtttca
    38821 acttcagatc attggagaag ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac
    38881 gtggttattc accgcccaat tttcggcggt gaactttact cgttttagcc attaattacg
    38941 agctttaaaa aaactcattc aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag
    39001 acgggcgaag ttatcggtgg ttacctgcgc cagttcttca acggcaacac ctttcaacac
    39061 agccatgtat tctgcaacgt cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg
    39121 taccggcgca aggtaaggtg agtcagtttc caccagtaat cgatccaggg ggacataacg
    39181 cgcagcatcg cgcagttgct ccgcattacg gaaggtcaca atgccggaaa aggagatgta
    39241 aaatccgaga tccagtaatt tacccgccgt ttctctgtcc tctgtaaaac agtgtagtac
    39301 gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc agcgtatcgg cacgggcgtc
    39361 acgggtatgg acgataaccg gcttgttcag ttcacgacca atctggatat gatggatgaa
    39421 ggactcttgc tgacgtactt tagtttccgg tgtgtaataa taatccagcc cggtttcacc
    39481 cagcgctaca acaccctctt ctgccgccag acggcgtaaa tcttctacat cgtagggatc
    39541 attctggttg agtggatgca cgccacaaga aaatacgacg ttgtcacgtt cgcctaccag
    39601 atcccacata tgtaaataac ccggtaatgt tgtggcgact gccagacaaa atttcacatc
    39661 gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta tgcaaagatt cataatccag
    39721 accatcgaga tggcagtgtg agtcgactaa aaacatgatg tctctcttaa agatgaggaa
    39781 ccggtagcac aacgcccggt tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga
    39841 gaagctcgcg gttgatgcct gtaacagaca ttaactgttc acgaatgtgg caaacatccc
    39901 ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc cagttcggcg accaggcccg
    39961 gcacatcaac attggtcacc tgcgcagcac catgatggcg ttttagcgca tccatcagca
    40021 acgttgccag ccagtgtaaa cgcgccggag cttgttcatg attaagggcc gctagcagcg
    40081 aataccagtc gcccgatggc acgctatatg ccaacgcctg acacaatgtt tcacgagcct
    40141 gccagttatc tccctgaaac aacgccagtg ccgcgccagg cgaaccggcg cttaagcgca
    40201 atgcggcaag taatgcatcc tgtgacattg tcacttcgcg tgaaagccag gtcacggcgt
    40261 actgttccgg cggcggcgca aggtaatgta accgacaacg actacgtaat gttgccagta
    40321 aacgttcagg ctcgcgggta gccaggaaaa accaggtttc tgctggtggc tcttcaagcg
    40381 ttttcagcaa tgcgttagcc gcggcgtcgg ttagtaaggc agcatcggtt acccagacaa
    40441 ctttcgcacc acctaagcgt gcgtgctcat tcagcttttc ggtgacctca cgtaccgcat
    40501 caatgcccaa cgcatttttc cctttttcgg gagccagggt gtagtaatcg ggatgcgtgc
    40561 cagcctgcat caactgacat ccacgacagt gaccgcaact tttgtggccc tgcggttgtt
    40621 ggcagagtaa ataacggctc agggcgtaga ttaaagcatc atcgcccatg cccggtaacg
    40681 cctgaatgag tagcgcatgg tgacctcttc cggcctgata gctggctacc agtttttcga
    40741 aatcaggtcg taaccatgga taccatctca tgcgtccaac tccttaaccc agtgggtcac
    40801 ggtagtgcgg attgcatcca tcacggcctc cagcggctgg gtggcatcaa tggtatgaat
    40861 acttttatct tgtgcagcca gttccagata gcgggcgcgg gtgcgattaa agaaatcgaa
    40921 agactcttgc tcaatacgat ccagctcgcc gcgcgcacgc gcgcgtttta agccaacttc
    40981 cggggtaaca tcgagataga gcgttaagtc ggggcgaaaa tccccgagaa cagcatcacg
    41041 cagtgttgcc agcatatgtt ggtcaatacc acgtcctccg ccctgatacg cctgagtgga
    41101 gagatcgtgg cgatcgccaa tcacccaggt gccgttagcc agagctggtt tgatgaccgt
    41161 ttctaccagt tgaacgcgcg cggcataaaa catcagaact tcggctttat cggtaatgac
    41221 ttcatcgcct accgatttga tatccagcac caggcttctt aacttttcgg caagttgcgt
    41281 accgccaggt tcccgagtga aaaccatgtc gcggataccc agttgctcaa gcgtctcaac
    41341 caccacatta cgcgcggtag ttttgcctgc gccttccagc ccctcaatga cgatatactt
    41401 actgcgcatt tttttcctta agcactttca gataatcctg cacagacttg ttatggctgg
    41461 caagattggt attaaacgtg tgaccacctt taccatcggc cacaaaatag agatacggcg
    41521 tttttgccgg atgcgcagca gccttcagcg aatccgcccc cggcgtagct atcgcacccg
    41581 gcggcaaacc ggtaatggta taggtgttat acgctgtcgg cgtttccagg tctgcacgag
    41641 aaagtttgcc attataacgc tctcccatcc cgtaaatcac ggttgggtcg gtctgcaggc
    41701 gcataccaat gcgtaaacgg ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa
    41761 cggcggtttc tttttcgata attgatgcca tcgtcaccag ctgattttta tctttataag
    41821 gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgcg
    41881 ctcgcttgag taacgcgaca tcggtggtat tggcggtata catccaggtg tctggccaga
    41941 accaaccttc aatccactcc gggttttcca gttcaagtgc ctgcgctacg gtggcgtact
    42001 tatcgtcact cagcgtgtgc ttgatatacg gagcctcacg caattgcttg aggtagtcgc
    42061 tcagacgcat cccttctacc agtcgcagcg ggaactgtgc ttctttaccg ctttccaaca
    42121 atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt
    42181 gagaaagatc cggttcgata cgcagcagcc attgaaaaac ccgtgggcga ttgatgatct
    42241 tatcagcata aagctgttca ccgagcgcca gacgtccggt ccctggcttc agggtaaata
    42301 tcgtctcttc tttgataagc aatttgctgt cggcaagatg gcgaaccttc cagacgccca
    42361 caccagcggc gatacccagt accaccagca ataacaagat tatcaataac acttttttca
    42421 tgactaattc gggcgctcac aaagtggggc taaatattca tataacgttg ctgacgaaaa
    42481 ggagacatcg ccacaggcac gtacgggcat cactggcatt aacgcattac aaataaccat
    42541 ctcatctgcc tgcaacgcct cttccagaga agcttgcact tcgacaagct gataagagga
    42601 ttgtgccagc aaacggatac agaattgtcg cataatgccg ttaacacctg cctgatccag
    42661 tcgcggcgta taaactacgt tgcccttccg ccagaacaaa ttagccgcac agcattccgt
    42721 aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct caagatgaga
    42781 gcgaatcaat acttgctcaa gacgattgag atgtttaata cctgcaagat gaggattgcg
    42841 ccccagccgc accgggctta gcgccaacgt catcccctcg ttacgcaaac ggtcgtaatg
    42901 tgcaggataa gccgtaacgg agagaatccg cgttgccggt ccactgttca atgtgctgta
    42961 ccctcgcccg ccactaccgc gactgatcac gactttcagt acaccatttt gctgttctgc
    43021 tgccagcgtt ttcatctctt gttcaagctg tggccagaag tcacaggaaa tcatcaaccg
    43081 ctgacaagca tcctgtagtc gctggatatg cgccgataac aaactgactt taccgtcgat
    43141 aactctggcg gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc ttactgccag
    43201 cgattcctgc ttataaccgt taattaagaa cataccggct ccttatcaga gaattgttag
    43261 tgtggcagca tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa
    43321 gcggaccttt tatatgggtg gaaaatgaca acttagatct ttttaaagat caaagaacca
    43381 ttagtgccac cgaagccgaa ggagttacac agcgtgtatt ccattccgct gacctgacgt
    43441 gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg ggttatccag gttgatggtt
    43501 ggcggaacag cctgatcgcg cagcgccagg atggagtaga tagattctac tgcacccgcc
    43561 gcacctaaca ggtgaccggt catagatttc gtggagctta ccaacacacg gcttgcagct
    43621 tcaccgaaga tggttttcac cgcctgcgct tcagctttat cgccagccgg cgtagaagta
    43681 ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc ctgcatcacg cagagcattt
    43741 gccattgcca gagctgcgcc tgcgccattt tctggcggtg acgtcatatg ataagcatcg
    43801 ctgctcatac caaagccgac gagttcagcg taaattttcg caccgcgttt tttcgcgtgt
    43861 tcgtactctt ctagtaccag cataccggca ccatcgccca gtacgaagcc atcacgctct
    43921 ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc
    43981 gcgccaaaac caccaacgcc cagcggcgta ctggctttct ctgcgccacc tgcaaccatc
    44041 acgtcagcat cgccatacgc gataatacgc gcagcatgac caatgttgtg cacgccggaa
    44101 gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc catacatgat agtcagatga
    44161 cctgccacca tgttcacaat cgttgacgga acgaagaatg ggctgatctt acgtggacca
    44221 ccgttcatca gagatgtgtg gttttcttcg atcagtccga ggccgccaat cccggagcca
    44281 attgcggcac caatgcgggt tgcgttctct tccgttattt caaggccaga atcctgcatg
    44341 gcctgaacgc cagcgacaat tccatattga atgaaggcat ccatcttgcg ctgttctttg
    44401 cgcgagataa tgtcctcaca gttaaaatcc tttactaagc cagcaaattt cgttgcatag
    44461 gcgctagtat cgaaatggtc gattaggctg atgccactct gaccggcaag cagagctttc
    44521 caggtagact ctacggtatt gccgacagga gacaacatgc ccagtccggt cacaactaca
    44581 cgacgcttag acacgtttgt cctccaggga gggaaaaaat gattctagtg ggacaaaaag
    44641 ataaaactca ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg gtggccgttg
    44701 atgtaatcaa tggcagcctg aacggtggtg attttctcag cttcttcgtc cggaatctca
    44761 gtatcaaact cttcttccag agccattacc agctcaacgg tgtcaagaga atccgcgccc
    44821 aggtcttcaa cgaaagaagc attgttggta acttcttcct gcttaacgcc cagctgttcg
    44881 ccgataattt tcttaacgcg ttcttcgata gtgctcatac tcttaaattt cctatcaaaa
    44941 ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga aaaatttgca actaaatccc
    45001 ggcaggtctt accacgattt tacgttattt tgaggcctta ccccccaata acgcaaatca
    45061 ttttgcacta attattactc ataaccacgc agactgcgcg caacatgagc attttgtgca
    45121 aatcgcggtc agaccatgta catcccgccg ttcacatgca aagtttcacc cgtgatgtaa
    45181 gctgcttcgt cggatgccag gaatgcaacc gcgttggcga tttcctgtgc gccgccgagg
    45241 cgacccgcag gaacctgcgc caggataccc gcacgctggt catcgctcag cgcacgtgtc
    45301 atgtccgttt caataaagcc cggagcaaca acgtttacag taataccgcg tgacgcaact
    45361 tcgcgcgcca gtgatttact gaagccgatc aagcccgctt tcgccgcagc gtagttggcc
    45421 tgaccgccat ttcccatggt accaaccaca gaaccgatag tgataatacg accatgacgc
    45481 tttttcatca tagcgcgcat taccgctttt gacagacgga aaacagatga aaggttggtt
    45541 tcgataatat cgttccactc ttcatctttc attcgcatta acaggttatc acgagtgata
    45601 ccggcattat tgaccaggat atccacttca ccaaattctg cgcgaatttt ttccagaaca
    45661 gattcgatag atgccgggtc ggtcacattc aacatcagac ctttgccgtt agcacctaaa
    45721 taatcactga tcgcctgagc gccattttca ctggtcgcag tgccaataac tttcgcgcca
    45781 cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc ggttaccagt
    45841 gcgatttttc cttcaaaatt catgattttc ctcttttaaa gctcgagcgc cgctgccatc
    45901 gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt cgtcaggcca
    45961 gttagcactt tgcccgggcc gacttcatag agatgttcta cgccttgcgc tgccatgtac
    46021 tcaacagact tcgtccactg aaccgggtta tacaactgac gtaccagtgc gtcacgaatg
    46081 gcatcaccat tggtttcgca tttcacatca acgttattca caacaggaac tgttggtgcg
    46141 ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg ctgctggttt catcagcgca
    46201 cagtgagacg gtacgctcac tggtaacggc agagcgcgtt ttgcgcccgc agctttacag
    46261 gcagcgccag cacgctcaac cgcctcttta tgaccggcaa taaccacctg tcccggagag
    46321 ttaaagttta ccggagaaac gacctgacct tctgcagctt cttcacacgc tttcgcaata
    46381 gacgcatcat ccagaccgat gattgccgcc atagcgcccg tgccttccgg tacggcttct
    46441 tgcataaact tgccgcgcat ctcaaccaga cgcaccgcat cagcgaaatc aatcacacca
    46501 gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg ccatcattgc cggtgcttta
    46561 ccgccctgct gctgccatac gcgatacagc gcaacagagg cagtcaacag cgcaggctga
    46621 gtttgccagg ttttattcag ttcttcagct ggcccctgct gggtcagcgc ccacaggtcg
    46681 tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga caattggata gctcgccgcc
    46741 atatcagcca gcattccaac ggtttgagaa ccctgtccag ggaacacaaa tgcaaattgc
    46801 gtcatgtttt aatccttatc ctagaaacga accagcgcgg agccccaggt gaatccaccg
    46861 ccaaaggctt caagcagaac caactgcccc ggcttaatgc gcccgtcgcg tacagcttca
    46921 tccagcgcgc acgggacaga ggccgcagag gtattaccgt ggcgatccag cgtcaccacg
    46981 acattgtcca tagacatacc gagttttttc gccgttgcac tgataatacg caggttagcc
    47041 tgatgcggaa ccagccagtc cagttgagaa cggtcaagat tattcgccgc cagcgtctca
    47101 tcaacgatgt gcgccagttc cgttaccgca accttgaaga cttcgttgcc cgccatcgtc
    47161 agatgaattg aattctctgg attcacacgg tcagcattag gcagcgtcag caactcacca
    47221 tagctaccgt cggcatgcag atgggtggag atgattcccg gctcttcaga ggcagccagc
    47281 accgcagcgc ccgcgccatc gccaaaaata ataatagtcc cacgatcggt tggatcgcag
    47341 gtgcgcgcca gtacatcgga accgacgacc agagcatact tcaccgcccc agatttcacg
    47401 tattgatcgg ctacgcttaa tgcataggtg aaacctgcgc aggctgctgc aacgtcaaat
    47461 gccgggcaac ctttaatgcc cagcatgctt tgaatctgac aagctgcgct cgggaaagcg
    47521 tgcgtagcag aagtcgttgc cacaacgatc aggccaatct ggtctttctc aatgcccgcc
    47581 atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc gtttggcgcg
    47641 gcaatgtggc gttcgcggat accggtacga gtgacaatcc actcgtcaga ggtgtccacc
    47701 attttttcca aatcggcgtt tgtccgcact tgttcgggca gatagctgcc agtaccaata
    47761 atcttcgtat acatgtacgc tcagtcactt ttcggttata taccgtcact tgcaaactgc
    47821 gagttcgctg gcagcgtcct gctaccgcag agttccgctt ttgccaccgt ccagcagctc
    47881 aaaaccagct gggtatacag attccaggcg agcggcaatt cgctgaggaa cttgtcgctg
    47941 caccgcctgc actgcctgtt caatcgcgac cgcaaaagct cgctgattgg ctgcaccatg
    48001 actttttatc accgtgccgc gcaatcctaa cagacaggcg ccgttatact ggtcggggtt
    48061 gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa cgctttaata acagtagcca
    48121 ccacgaccgt tttttccctt caccctgaga tttcagcaga gaaaggaaca tcctgacaac
    48181 accttccatc gtctttaacg tgacatttcc tgtaaagccg tcacaaacca gcacatctgt
    48241 cttgccagtt aacaactcat tggcttcaag atagccgata taattgatag aagggattgt
    48301 tttaagcacc gctgaggcat cccgaatact gtcgagaccc tttacttctt cttcaccaat
    48361 attgagcaac gccacgcgag gattgggaat ttccaccacc tcttcagcca gcactgagcc
    48421 cataatggca aattgcacca acattgtgct gtcacaatcg acgttggccc ctaaatcgag
    48481 aaccaccgtt ttgccctttt gctgatgcgg caataccgtc accagcgccg gacgctcaat
    48541 cccctccagg ggcttgagta ataattttgc cagccccatc agtgccccgg tattaccggc
    48601 actgacacag gcttgcgctc gaccttcttt caccaactcc agggccacgc gcattgaact
    48661 cccacgactg gcgcggatag cttgcgaagg ccggacatca ctggcgataa ctgactgcgc
    48721 aggaataatc tgcagacgcg aacgttgttc aaagtcagct ttagcaagta atggcgtgat
    48781 ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa ttagaattca gtgcctgcaa
    48841 tgctgcaggc actgtcacgg aagggccaaa atcccctccc atgacatcta acgccagggt
    48901 tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc ctggggaaaa
    48961 tcctcactaa gcttcatcac gcagacgcgt gattacttag cgatgacctt gcggccgcgg
    49021 tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt caccagaagt tttgtctaca
    49081 gacaggctgg tgactgcggt cagcgcgtca tgggaacgac gcatgccacg tttggaacgg
    49141 gttggtttat tctgttgtac ggccatggac cttactcctc aattacggtt tatccccgat
    49201 ccaacgggga gcaccaatta cttacgcttt aagctggcta atacggcaaa tgggtttggc
    49261 ttttgcgctt cttcaggcag ttcaccaaag accatgtccg cttcggacac ttcacagtgt
    49321 tcagaatcat gcaccggaac taccggcaag gcgaggatga tttcatcttc aaccattgca
    49381 agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc cggcagtgct
    49441 tcagcctgtt cgtctgaacg cacaggacta aaacaatacg ttgtgtagac ctgatgagta
    49501 aacggcttcc cgcaacgctg acactcgagc gttaccgtca ccttcgcatc gccgtttaac
    49561 actgcgagac gttggttatc gatagcgaac gacatggagc attccacatc actgtccaca
    49621 ctgactacgg attcggcgac gcgctcaacc tgatcagggg tatagatacc ctggtaatca
    49681 aggcgttttt gagccgtacg aaccggatcg agagtcaggg gtaattttac cttttgcata
    49741 gggcgcgcat attaactttg taacgtcata gagtcaaaga aaaaggcagc ctgtggttgc
    49801 cttttgccaa taattcgcac acattgcggg ttactgcttt attttcgttc agtgctgata
    49861 acgaacatca acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc aatacgctat
    49921 atctgatgga aaaaaatatg cctaaactta ttttagcctc cacctcgcca tggcgtcgcg
    49981 ctcttctgga aaaactgcaa atctcttttg aatgtgcagc accagaggtc gacgaaaccc
    50041 cacgcagcga cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa aaagcgcaat
    50101 ctctggcgtc acgttatccg gatcatttaa ttattggttc agaccaggta tgtgttcttg
    50161 atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc ccgtctgcaa ttacgcaaag
    50221 ccagcggcaa tatcgtcacc ttctatactg gactggcgtt gtttaattcg gcgaatgggc
    50281 atctgcaaac agaagtggag ccttttgacg tccatttccg tcacctgagc gaggcggaga
    50341 ttgataatta cgtgcgtaaa gagcatcccc tgcactgcgc gggtagcttt aagagtgaag
    50401 gatttggcat tacgctgttt gagcgtttag aggggcgtga tcctaacacg ctggttggtt
    50461 taccgctaat cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg ctgatgggat
    50521 agttttttgc gccggatgcg gttacttcgc atccggcaat cggtgctggc ggcctgatgc
    50581 ggcgtaaacg ccttatcagg ccaaccgccc attatcaggt ttatatgctt gtattagcgc
    50641 gcgttacgca gcttttgcaa acaacgcttc aaaccttcat ccatcggagc ttcgatacgc
    50701 atcacctcac cggtccccgg atgagtaaac ttcaacgctg cagcgtgcag gaacaggcga
    50761 tttaatcccg tgcctgcttc agtgagctgc ctgtcaaatt cacggtcacc gtagcgatcg
    50821 tcaaaggcaa tcggatgacc cgcatattgt gtatgcacac ggatctggtg agtacgccct
    50881 gttaccgggc tacaacgcac cagggtggca aatgcatagc gttcttccac tttaaagcgt
    50941 gtttccgacg gtttgccttc ctgactcaca cgcacgatac gttcgccgct ttgcagaata
    51001 tttttcagta acggtgcttg aacgctcttc acatgcgact gccactgacc gcgcaccagc
    51061 gccaggtaat ctttttgcat ccctttttca cgtaattgct catgcagaga acgcaacgcc
    51121 gagcgttttt tcgctaccag caaaacacct gaggtgtccc ggtcaagacg atgaaccagt
    51181 tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt caataacgcc gaagcttaaa
    51241 ccactgccgc catgtaccgc cgtaccggaa ggtttattca gcaccaggat gtgatcatct
    51301 tcatataaga tgacgtccgc cagcgccgcc accttttgca gatgtggcga aaccgcctct
    51361 tcttcccgct cagcaacgcg aaccggtgga atacgcacct catcacccgc ttcgagttta
    51421 tattcaggct taatacgttt tttgttcacc cgcacttcgc ctttacgcaa aatacggtaa
    51481 atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa agttatcgat acgttgcccc
    51541 gcttcgtcag cggtgatagc aacaattttt acggatggag tctctgtttt catggtgggc
    51601 gattctaatt agccaacagg attcgcgcca ctcatttttc tatgcttata tttagtttta
    51661 caccttatta cttcactacg tgatcacttt attgatggtt attaaaccaa tcaccagcaa
    51721 gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa aagtcatctt gctataacaa
    51781 ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc ttgttaaaac aagaaatttt
    51841 acggaataac ccattttgcc cgaccgatca tccacgcagc aatggcgtaa gacgtattga
    51901 tctttcaggc agttagcggg ctgcgggttg cagtccttac cggtagatgg aaatatttct
    51961 ggagagtaat acccagtctg tttctttgat aattgcgctg tttttccgca tgaaaaacgg
    52021 gcaaccgaca ctctgcgcct ctttgagctg acgataaccg tgaggttggc gacgcgacta
    52081 gacacgaggc catcggttca cacccggaaa ggcgttactt tgcccgcagc ttagtcgtca
    52141 atgtaagaat aatgagtaag ttacgatgaa aagaatgtta atcaacgcaa ctcagcagga
    52201 agagttgcgc gttgcccttg tagatgggca gcgtctgtat gacctggata tcgaaagtcc
    52261 agggcacgag cagaaaaagg caaacatcta caaaggtaaa atcacccgca ttgaaccgag
    52321 tctggaagct gcttttgttg attacggcgc tgaacgtcac ggtttcctcc cactaaaaga
    52381 aattgcccgc gaatatttcc ctgctaacta cagtgctcat ggtcgtccca acattaaaga
    52441 tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat aaagaagagc gcggcaacaa
    52501 aggcgcggca ttaaccacct ttatcagtct ggcgggtagc tatctggttc tgatgccgaa
    52561 caacccgcgc gcgggtggca tttctcgccg tatcgaaggc gacgaccgta ccgaattaaa
    52621 agaagcactg gcgagccttg aactgccgga aggcatgggg cttatcgtgc gcaccgctgg
    52681 cgtcggcaaa tctgctgagg cgctgcaatg ggatttaagc ttccgtctga aacactggga
    52741 agccatcaaa aaagccgctg aaagccgccc ggctccgttc ctgattcatc aggagagcaa
    52801 cgtaatcgtt cgcgcattcc gcgattactt acgtcaggac atcggcgaaa tccttatcga
    52861 taacccgaaa gtgctcgaac tggcacgtca gcatatcgct gcattaggtc gcccggattt
    52921 cagcagcaaa atcaaactgt acaccggcga gatcccgctg ttcagccact accagatcga
    52981 gtcgcagatc gaatccgcct tccagcgtga agtccgtctg ccgtccggtg gttccattgt
    53041 tatcgacagc accgaagcgt taaccgccat cgacatcaac tccgcacgcg cgacccgcgg
    53101 cggcgatatc gaagaaactg cgtttaacac caacctcgaa gctgccgatg agattgctcg
    53161 tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc gacttcatcg acatgacgcc
    53221 tgtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa gcggtgcgtc aggaccgtgc
    53281 gcgtattcaa atcagccata tttctcgctt tggcctgctg gaaatgtccc gtcagcgcct
    53341 gagcccatca ctgggtgaat ccagtcatca cgtttgtccg cgttgttctg gtactggcac
    53401 cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt ctgatcgaag aagaagcgct
    53461 gaaagagaac acccaggaag ttcacgccat tgttcctgtg ccaatcgctt cttacctgct
    53521 gaatgaaaaa cgttctgcgg taaatgccat tgaaactcgt caggacggtg tgcgctgtgt
    53581 aattgtgcca aacgatcaga tggaaacccc gcactaccac gtgctgcgcg tgcgtaaagg
    53641 ggaagaaacc ccaaccttaa gctacatgct gccgaagctg catgaagaag cgatggcgct
    53701 gccgtctgaa gaagagttcg ctgaacgtaa gcgtccggaa caacctgcgc tggcaacctt
    53761 tgccatgccg gatgtgccgc cagcgccaac gccagctgaa cctgccgcgc ctgttgtagc
    53821 cccagcaccg aaagctgcac cggcaacacc agcaactcct gcacaacctg ggctgttgag
    53881 ccgcttcttc ggcgcactga aagcgctgtt cagcggtagt gaagaaacca aaccgaccga
    53941 gcaaccagca ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc gcaagcctcg
    54001 tcagaacaac cgccgtgacc gtaatgagcg ccgcgacacc cgtagtgaac gtactgaagg
    54061 cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag gcacagcagc agactgccga
    54121 gacgcgtgag agccgtcagc aagctgaggt aacggaaaaa gcacgtacca ccgacgagca
    54181 gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat gataaacgtc aggcgcaaca
    54241 agaagcgaag gcgctgaatg ttgaagagca atctgttcag gaaaccgaac aggaagaacg
    54301 tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc aatcagaaag tgcgttacga
    54361 gcaaagcgta gccgaagaag cggtagtcgc accggtggtt gaagaaactg ccgctgccga
    54421 accaattgtt caggaagctc cagctccacg cacagaactg gtgaaagtcc cgctgccagt
    54481 cgtagcgcaa actgcaccag aacagcaaga agagaacaat gctgataacc gtgacaacgg
    54541 tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg cgcgtgagtg gtcagcgtcg
    54601 tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca atgccgttga ccgtagcgtg
    54661 cgcgtctccg gaactggcct ctggcaaagt ctggatccgc tatccaattg tacgtccgca
    54721 agatgtacaa gttgaagagc agcgcgaaca ggaagaagta caagtgcagc cgatggtgac
    54781 tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc gcgccagttg ttgaagaaat
    54841 ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg caaccggaag tggttgaaac
    54901 gacgcatcct gaagtgattg ccgccgcggt aactgaacag ccgcaggtga ttaccgagtc
    54961 tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa ccggtggttg aaccgcagga
    55021 agagacggca gatattgaag aagttgccga aactgctgag gttgtggttg ctgaacctga
    55081 agttgttgct caacctgccg cgccagtcgt cgctgaagtc gcagcagaag ttgaaacggt
    55141 agccgcggtt gaaccagaga tcaccgttga gcataaccac gctaccgcgc caatgacgcg
    55201 cgctccggca ccggaatatg ttccggaggc accgcgtcac agtgactggc agcgccctac
    55261 ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg gcaacgcatc atgcctctgc
    55321 cgctcctgcg cgtccgcaac ctgttgagta ataattagct caaagtaatc aagccctggt
    55381 aactgccagg gcttttttat ttcatctttg aatcaacata ttaaaattca tcaaatggca
    55441 tccttgctaa ccaacaatgc aaaataggca aaaaaatgcc cgccccggcg atcccagagc
    55501 aggcagacaa aacataccca gtttcatgat atgttttaaa gcgagcttat ttgttgagct
    55561 ggaagagcga caatccctgc atatcggtaa atgctttata cgatgcctgc aatgccgttt
    55621 gctgcatgat gtaagatgaa atagttgcat tccagtccac atcaaccaga tcgctcatct
    55681 gctgcgtttg ccctaaagcg cgatcgctac ctaatgaatc cagcgactcc agttcgttca
    55741 gctgcgtgcc taattccgcg cgaactgtaa ggacattatt cagcgagttt ttcagtccac
    55801 ggttggtttt atccaatgcc gcagcggcga tttctttatc cgcttcgcta tccgcgaccg
    55861 gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa aagattggtt tcagaagcgc
    55921 taccgtctgg ttccgctacc gcgttactgg taatactgtc gaaaattttg tcgcccgtat
    55981 gtcctatcac catcgaacgc gaagcatcga cctgttgctt aatactttct gttccgccga
    56041 cgtatttccc tttctcttcg ctaaacggcg cagtctctgt tttataaccg gcaaaaatgt
    56101 agcgcccgtt accgtcagtg gtgttcgcca gattcagcaa ctggtcacga agcccctgga
    56161 tatccgtagc cagcgaggcc cggtcatcgt cactcaaagt gccattgctg gcgtagacaa
    56221 ttttttcctg agcattctgg atagcagtgg tgacctggct aagtacactc tcttccagtg
    56281 acactttttg agtggcgaaa gtacgcgcca gcgtgtactg gctgttttgc gcctgtgcct
    56341 gggagagaac aaccgcttgt gatgctgcaa tgggatcgtc agaagggtta acgactcgct
    56401 tacctgtcga catctgctcg ccgtacttca tccattccgc ctgagaattg gtgatgccac
    56461 gcatgttttg ctggtacatc atctgggtac tgaaacgcat cttatctccc cttagcgaat
    56521 gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta
    56581 atactgctga aaacgttgca gatttccgta ctcctcatcg agattgacac cggaaatcga
    56641 ctgctgctga ttggaaagct gcgtcaccac attaccttgc gtggtgctac tggttttcaa
    56701 cgtcgcggtt ttattaccga tatcactcac taacgaggca taagcgtcgt taaaggattt
    56761 cgcaccgccc accgttttac tgttgctttg cagatccagc agggcctgac cgttgcggtt
    56821 atcgctatca cccgcatctt cttcgctcgc catcgctatt ttcgcttcgt cggtgattaa
    56881 tacatccatg ttgacgatag cgtcacttac tggtttcagc gtgaagctgt cgttaacagc
    56941 aggcgttcct gtaaacgtca actccagacc atcaaatgcc actttaccgt tggcatccgg
    57001 cgtcaccgta aaagtggtat tactggcaag gcgggtgacc tgccactgat tattatcgaa
    57061 cgagattttg taatcagtcg ccagtaccgc ggaggcatca gttaccgtgg caccgatcgc
    57121 aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc ttaccgatag caaagaaatc
    57181 ttcaccggca tcgccgttag catcaaatcc ggctttgtgt tggctgttga aagcctcggc
    57241 aaatgccagc gccagttgtc caagcgtatt acgcgtctgg tccagatcct gagaacggaa
    57301 tgtcagaatg ccgcccagcg acccggtatt cagtaatttc tccgggatct caatattgcc
    57361 tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg tcggcgctgg aaggaactgc
    57421 cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa ccattggcca tcgtgatgtt
    57481 ataagtaccg ccatcctgaa cgctgacttc tacgccaaca atctggttta attcgctcac
    57541 cagttgatcg cgttgatcca gcagattgtt aggtgacgcc cctgccccca cgcctgtcag
    57601 gcgcgagatt tgatcgttca ggctggcaat ttgtttagcg taattgttga tctgatcaac
    57661 gctggcacct atcgcgatat tgacctgttt gtcctggtcg cgcagatatt gatcggtggt
    57721 tttaaactga ttcaccaacc cttctgattt cccaatcagc gcctggcgcg ctgccgggtc
    57781 ttccgcgtta ctcaccagcg tttgcaggct ggtgaagaaa tcctgcatct gtgttgccag
    57841 cgaagaggta ctggtggaga gcatattgtc gattttcgac atctgctcat agcgggcagt
    57901 caggccgcta ctttgcgtct gcgcagcacg taactggttg gtaataaacg catcatactc
    57961 acgctgcaca ccagaaacgt agacgccatt gccaacccag ccgccagcgc ccaacgtgct
    58021 attggcctgc gccataatag tggtttggcg ggtatatccg gcaacgttat agctggagat
    58081 attattactt gccgtattta acgccgcctg ggccgcgttc agtccgctca tggcgttatt
    58141 aatcaagctg gacatggagg ttccttataa gccttcaatt acagagtatt atcggcagcg
    58201 acccgccgga cttgagttat tcagaacaga ttatcaatgt tcatactgta ggttttgctc
    58261 accttgtcgc ttatcgattt catctgctga atcatgttgg tgagtttgcg ggcatagtga
    58321 ggatcggtgg catagcccgc gtcctgtagg gcctgcgccc cctgttccgc actcgcggcg
    58381 gtcgtcacgg cggcgtagcg cgggttacgc gttaacagcc caacgtaatc cgacaaggct
    58441 tccagatacg agctgtagac gcgaaacttc gcttttactt tcttcgcttc gccgttttca
    58501 tattcagtcg tggtgatttc agtaactggc cctttccagt tgccagaggc tttgacacca
    58561 aacaggttat agctcggctc gccgttttcg cggcggattt gccgttgccc ccaaccagat
    58621 tccagtgccg cctgagcgag gatcaaatga tgtggcacac cgctttgctg gcttgccagt
    58681 tgggcgggca gcgagagttg cgcgaggaat gctttactgt cacccggcag cgaatcatcg
    58741 tagttacgtg gcacggcctt ttgcaccagc tgcgaaagcg cctgattttg ataacgcacc
    58801 acagtttcga gcgggaattt catcggtgct gctggcgtgg actcctctgg caatggttgt
    58861 tctggcgtca tctgtttaac catcatctct gcaagcccca gacctttgcc cgccgtcatc
    58921 tgttgggcaa tttgctggtc atacatactg gtatacagac gagtgtgctc gctgctgaac
    58981 aggccatctt ttggtaaagc gtcgcgcatg cttttcaaca tcatctgcac gaacatccct
    59041 tccacctggc gggccaccgg acggatattt gccgccggat cttcgcccgc tttcgccttt
    59101 agttcgttga gtgattgcgc atcccaggcc gcacttgcca gtagtttgct gtcgctgatc
    59161 atcagatgat ttccagtttt gcccgcagac atcccgcact ttgcattgat tgcaaaatag
    59221 acatcagatc catcggcgta gcgcccagcg cattgagcgc gcgcaccacg ttattgaggc
    59281 tggcgctgga acgtacgctt tgcagcgaac cgccgctctg gcgtaaatcg atctgcgttt
    59341 gtggagtaac cacagtctgt ccaccaccaa acggtgtatc tggctggctg acattggcct
    59401 gacgattgac tgttactgag agatttccct gcgctaccgc gcagctgtcg agggtcactt
    59461 cgcgattcat caccaccgaa ccggtgcgcg agttaatcac tactttagcg tcctgcgggg
    59521 tgacattaac ctgcatattc tggatatctg caaggaaacg gacctgggaa ctgttgccac
    59581 ttggtacgcg cacctgaata gtccgcgcat ctaacgcggt ggcgctgcca tatccacgca
    59641 cgcggttgat ggtgtcagcg atttgctgcg ccatgctgaa atcttcgtcg ttaagttgca
    59701 aattaagggt attcccgacg ccaaactggc tgggcaattc acgttcaata accgcaccat
    59761 tggtgatccg tccaccgttc agttggttca cctgaacact gctaccgcca gcggaggctc
    59821 ctgcgccgcc aaccagaata ttgccctgcg ccagcgcata cacctgactg tcaacgccct
    59881 taagcggcgt catcaacaac gtacctccac gcaagctttt ggcatttccc atggaagaaa
    59941 ccaccacatc gatggtttgc ccctgacgtc caaacggagg aagtgacgct gtcaccatta
    60001 ccgcagcgac gttttttagc tgcatattgg tgcccgtcgg aacggtaatt cccagctgtg
    60061 agagcatgtt attaagcgtt tgtgtggtaa acggcgtctg ggttgtctgg tcaccggtgc
    60121 catccagccc caccaccaga ccatagccaa tcagtgagtt ttgccttacc ccctgaacac
    60181 tggtgagatc gcgaatacgc tcagcctgag ccgccgtcgt gaccagtaga agaattaatg
    60241 cagagagaaa tttaatcacc acagcctcac ttacattggc gacaggttaa ggaagaaacg
    60301 ctgcaaccag cccatatttt gcgcttcgtt aatgtagcca ttgcctacgt attcaatgcg
    60361 cgcatccgcc acctgagtag acggtacggt attgctgccg ctgatagtgc gtggattaac
    60421 cacgccagag aagcgaataa attcggtacc ctgattaatg gcaatctgtt tttcacccac
    60481 cacatgcagg ttgccgttga ccagtacctg gtcaaccgtc accgtcaacg tgccgctaaa
    60541 ggtattgctg gcattggccc cgccctttcc gttgaacgtg ttaccaccgg aggcttcgac
    60601 atcggcacga gcgttaccaa acagcccctg caaatagcgc ggcacagtat caaagccaaa
    60661 attagtttta ccgtcacggc tggcattcgc agaggagctt ttgctggcgc tgacgttctc
    60721 ctgcaacacg atggtcagcg tatcgccaat attgcgtggt cgacgatctt caaacagcgg
    60781 ttgatagcca tagttaatcg gctgagcaga ctggaaaata gaaccgttgg cgacgggcgt
    60841 cggaccggga accggttgtg cactggtcgc cccctgcacc agcggcgtgg agggtatcca
    60901 ggcgcagccg gttagtgaaa gcaccaacaa gctggaaatg gcataagtat gcgcagcgtt
    60961 tttttgcatg gctatcatct tcaaaaatca gtaaaccggt gaacctgcca ccggttaagc
    61021 cttagagttg cgtcagtttt tgcagcatct gatcggtggt ggacaccgct ttactgttga
    61081 tttcgtaagc gcgttgcacc tgaatcatat tgaccagttc ttccgccacg ttgacgttag
    61141 acgtttcaac atacccttga tacagcagtc ctgcgccgtt caggcccggc gtgctttcgt
    61201 tcggtgcacc agaggattgc gtttcggtgt agaggttttc gccaatgctc tccagcccgg
    61261 tgtcattcat aaaggtggtg agattgagct gcccaacctg aaccggagct gcctggcctt
    61321 gttgggttac gctgaccacg ccatcacgac cgatggtgat acttaacgca ttcgccggaa
    61381 tggtgatcgc tggctgcacc tgaaaaccac cagccgtcac cagctgcccg ttctgatcca
    61441 cctggaaaga gccgtcacgg gtataggctg atgaaccatc tggcaacatc acctggaaaa
    61501 agccctgccc tttaatcgcg acatctttgc tgttgttggt ctgcgacagg tttccctggc
    61561 tgtgtaagcg ttcagtggcg accgggcgta cccccgtgcc gatttgtaat ccggagggta
    61621 aggtggtttg ttcggaagac tgtgcccccg gctggcgaat ggtttgataa agcagatctt
    61681 caaacaccgc gcgctgacgc ttaaaaccgt tagtactgac gtttgccagg ttgttggcaa
    61741 tgacgtccat attggtttgc tgggcgtcaa ggcccgtttt ggcgatccat aatgaactga
    61801 tcataaaatg tcctgtagtg gattagtgac aaagtgcata tgttttcttg ccggatgcgg
    61861 cgtaaacgcc ttatccggcc tacaagagct cgcaaaatca ataaattgcg attgccccca
    61921 tagacccgat aagcttgcgc atcgggcaac ttatacttgt catgtatcct ttcaattaac
    61981 tcatcgacag cagttggttg gcacggcctg cgttatcatc gacgctgctg atcaccttca
    62041 tctgcatttc aaaacgccgc gcgctggcaa tcatgtcgct cattgccgca acggcattga
    62101 cgttactgcc ttccagaacc cccgacatca cacgcaaggt tggatctgcc tgcagtatcg
    62161 gcccacgcgt ggcctggctt tctgcgctta aacgaaaaat gccgtcgtca ccgcgttgca
    62221 cttcgctgcc cgtggctttc accagtttca gacgccccac tggcgcaacc gtatttgccg
    62281 gatcgcccgg attgagcgcc gagattgtgc catcggcagc aatagtgatt tccgcccctt
    62341 ccggcacagc aattggccca gcctcgccta tcaccggatg cccctgaatt gtcagttgcc
    62401 cggtgggatc aacctgaatg ctgccattac gcgtataccc ttcgctgccg tcagcggact
    62461 gcacggccag ccagccatcc tgctgcaatg caacgtccag cgggcgcgag gtgtaatcca
    62521 tcttgccggg cgtcatgtct gcacccggtg ttgacgccgt taccagcgtg cgcgtgggca
    62581 gtgaaagccc ttccaccggc actgcacgca aggcgtttaa ctgcgcgcga aaaccgggcg
    62641 tagaggcatt ggccagatta ctggcggtta ccgcctgttg attcagcgtc tggctggctg
    62701 ctcccatcgc ggtataaatt gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag
    62761 ttaaccagcg tgttgaggat ctggtcctgg gttttgatgg tctgggcgtt agactgatag
    62821 ttacgctggg caacgatcat attgaccagt tctttactga gatcgacgtt ggacgcttcc
    62881 aacgcaccgt tggtcagggt gccaaagttg cccgtcccgg ctgtccccaa cagcgccacg
    62941 ccagaagatt gcgtcgcaga ccagacgttg tcgccttcgg atgccagacc ttcgttgttg
    63001 gcaaagttcg ccagtacaat ctgccccagc agttgtgttt gttcgttgga atagttgccg
    63061 acaaccgtac cgtcatcatt gatttgataa ctcaccagat cgcccggttt gtagccattc
    63121 tgggtggttg ccacaatatt gttagcgccg gtattttgct gcatggagtt gaggaagctc
    63181 aggctaaacg tggcgggatc tgcgccgtta attgcgccag tggtaatatt ctctgttgga
    63241 tttgaggtca gaacgccatt ggcattaaac accagcttca ttgcaggctc ggctgtacct
    63301 gttggatcac tgctatcctg ggtgtagacc tgccagttat tatccccggt cttcacaaag
    63361 tagacgctca tgtcatgagc attaccctga ctgtcgaaaa cagtcaccga accttttttg
    63421 ttatagctat ccgcattgct ggcgctgaat ggcgtaacag taggaagcgg atcactggaa
    63481 ttcaggttga tctgcatcga tgccgtggtg gtagttttcg ctgccatcag ggtattcggg
    63541 atcgaaatat tggtcggatt cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg
    63601 taacccgtca gctgtaaacc ttgcatattc accaggttac ggttttcatc cagcttaaat
    63661 tgtccgttac ggctgtagaa caccgaaccg ttgctgtcta ccagacggaa aaaaccgttc
    63721 tggctgatag caacgtccag acctcgcccg gtgttggtgg tcgtgccatc ggtaaagtcc
    63781 tgagtgatac cggcaacttt tacccccagt cccactttcg aaccggcaaa catatcggca
    63841 aaagaggccg tgcctgattt aaagccgtag gtggcggagt tggcgatatt gttgccaata
    63901 acatcgaggt tggtggcggc agcgtttaat ccgctaaccg cttgagaaaa ggccatgact
    63961 gactcctgaa atgtgaaggc ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg
    64021 taagtgccga gatccagcgt attaccgctg ttgccgcgga tcacaccctg caccagagca
    64081 aactgcagcg gctgggcaac cagttgtgta ccaccgttac tggcgctaat cgctacattg
    64141 taagaaccgt tcggcgcagt gctgccatca gtcaacgtac cgtcccaggt gaaactgtga
    64201 actccagcgg tcagttcacc aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg
    64261 atggtggccg taactttgtc tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg
    64321 gtcacagccc cttcttcact gccggttccc gcaagaacag ttgtgccggg gatcatcacg
    64381 ccgtgaccga tcaggttact ggcctgtaac gactggctgt tatcaatctg tccggaaata
    64441 gatccgagcg tggtattgag tttttcaatc ccgctgaccg tgctgatttg tgccaattgc
    64501 gacgtcagct cgttgttttc cattggattg gtcgggtcct ggtttttcag ctgcgccacc
    64561 agcaaagtca gaaaactgct ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg
    64621 ctggtggtac tgacgccggt atttgtcgga tcggtggtgg ttaccgcaat ggacatagct
    64681 ttctccttta ttgaccgagc gtaagggttt tcagcatcat acttttcacc gtgttgagca
    64741 cttcaacgtt ggcctgatag ctgcgtgacg ccgacatggt gttaaccatc tccccgacga
    64801 catcaacgtt cggcattttt acgtagccct ttgcatctgc cagcggatta cccggttcat
    64861 aaaccagttt gtccggggcc tgactttcta taacatcggc aacctttacg ccgcctgtcg
    64921 cagcacctgg tgcagcgtta acctggaata ccacctgttt tgcccgatat ggctgtccat
    64981 caggaccggt cacgctatca gcatttgcca gattactggc cgccacgttc aggcgctggg
    65041 actgggcagt taacgccgac ccggcgatat caaaaatatt cagcagtgcc atccgttaat
    65101 ttccgctctg taaaacgttc atcatgcctt tgatttgccc gctcaacgcg ctaaggctca
    65161 tctggtattg caggctgtta tcggcaaact gggtgcgttc gcgatccata tcgacggtat
    65221 taccgtcaag cgaaggctgg tccggaatac ggtattgcag ttctgcggta ggaggcgtca
    65281 gcgcctgcgc cgggatgtgt tgcgttgagg tcatcgtcag tgcaaccaca ctggttgcat
    65341 cccgtccacg ttgcatgact tttttaagtt cactggcaaa atcgatatcg cgcgcctgat
    65401 aaccaggggt atcggcattg gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca
    65461 gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag cttatcgagc atatctcctc
    65521 cgcaggtatc aaaattctgc catcagctta aatgccttta cgcgcgcgtt atcggcggaa
    65581 taaacgcaaa atgggtcgct atttatgccg ttgatggtca ttgcggacag gtacaattca
    65641 cgttgtagaa atggctgggg gcgaaaatgc tgacaataaa acgtagcgtg gcgatcatcg
    65701 cgatactgtt cagtccgtta agtacggcga gcaatctcac atcgcaattg cacaactttt
    65761 ttagcgccca actcgcgggg gtaagtgatg aggttcgtgt ttctattcgt acagcgccca
    65821 atctactacc gccatgcgag cagccattgc tttcgatgag caataattcc cgcctgtggg
    65881 gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg atatttacag gttaatgtgc
    65941 aggccacagg aaattatgtg gttgccgcga tgcccattgt gcggggagga aagctggaag
    66001 ctggcaatgt caaactaaaa cgcggacggc tggataccct gccaccgcgt acggtgctgg
    66061 atatcaatca acttgttgat gccattagcc tgcgcgatct atcacccgat caacctatcc
    66121 agttaaccca gtttcgccag gcatggcggg taaaagcggg acaacgcgtc aatgtgatcg
    66181 ccagcggtga tgggtttagc gccaacgcag aaggtcaggc gctgaacaat gcagccgtcg
    66241 cacagaatgc gcgggtgcgc atggtatcgg gacaggtagt cagcggcgtt gttgatgcag
    66301 atgggaatat tcttataaac ctgtaagctg ttaaagatta cccgtccctt gccgataaat
    66361 aagcaacaca tgataaaagc gccctcaatg aggaataaac catgagtatt gatcgcactt
    66421 cgcctctgaa gcctgtaagc accgttcaac cgcgcgaaac cactgacgcg ccggtaacga
    66481 acagccgggc ggcaaaaaca accgcctcca ccagcaccag tgtgacgtta agcgacgcgc
    66541 aagcaaaact gatgcaaccc ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa
    66601 aactggcgat tcgtaacggt gaactaaaaa tggacaccgg caaaattgcc gatgcgctga
    66661 tcaacgaagc gcagcaagac ttgcagagta actgatcgta tgacacgtct tgcagagatc
    66721 ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg taatggatca agagcagcaa
    66781 catctctcta tggggcagat caacggcagc cagttgcaat ggattacaga acaaaaaagc
    66841 tcactgctgg cgacgctgga ttacctcgaa cagttacgca agaaagaacc caatacagca
    66901 aatagcgttg atattagtca acgctggcag gaaattactg tgaaaacgca gcaactacgc
    66961 caaatgaatc aacataacgg ctggttactg gaaggacaga ttgagcacaa tcaacaggcg
    67021 ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg gggcgaacgg tcagacctca
    67081 acaacccatc gcggcggtaa aaagatttcg atctgaatat tatctccggc ctgcactgca
    67141 ggccggaatg cattgttaca ccgtccggcg ggcaaattct ttaactttga agcccagtac
    67201 cgccagtgca gcgaagtacg cggcaatccc cgccagcacg accgccatta aacgcagtaa
    67261 gcgccagggc atggtaccca atgaccactc cggcatgata tgtaacatac ctaaaagcac
    67321 gccagacatc accagtaccg ccaccaccag acgcaacaga aacgccatcc agccgggttg
    67381 cggggtaaag attttctgct tacgcaactg ccagtaaagc agcgaagcat tcagacacgc
    67441 cgccagacca atagaaagtg acagcccggc atgtttcaac ggaccaataa acgccaggtt
    67501 catcaattgc gttaaaatca gcgtaacgat ggcaattttc actggcgttt taatgtcctg
    67561 gcgggaataa aagccaggag ccaaaacttt cactacaatc aggccaatca aacccaccga
    67621 gtaggcaatt aacgcccgct gggtcatcag cgcatcaaag gcggtaaatt taccgtactg
    67681 gaacagcgaa acggtcaacg gacctgaaag aatgcccaac gcaaccgcac tcggcagcgc
    67741 caacaggaaa caaagacgca gcccccagtc catcaaacgg ttgtattcat catgattgcc
    67801 actggcaaaa ctcttcgaca gcgacggcag caaaatggtg ccaagcgcca cgcccagcac
    67861 accagacgga aactccatta agcggtcggc gtaatacatc caagacaccg aaccggaagc
    67921 aagaaacgag gcaaaaatgg tgttgatgat taaggagatc tggctcacag agacgccaag
    67981 gatcgccggt cccatctgtt tcaccacgcg cattgctccg gcatcgtgga agttaatgcg
    68041 cggcaggacc agcatgccga tcttcttcag gtgcggtagc tgatacacca gctgcaggat
    68101 gccgccgacc gtaacagccc acgccagcgc cagcaccggt gggttaaagt acggtgcggc
    68161 aaacagcgcg aaaccaatca tgctgatgtt aagcagtgtt ggagcaaacg ccggaatcga
    68221 gaagcggttc cacgtattca gaatcgctcc caccagcgac gccagggaga tcagcaagat
    68281 atagggaaag gtaatcttta gtagctggct ggtcagggca aatttgtcgg cagtgtcagc
    68341 gaagcctggc gcggtcacca tgatcaccca cggtgcggcg agcatgccag cgaccgtcac
    68401 aaccgccagc gcgagtgtca gcaggccaga aacataagag acaaagaccc gcgtggcgtc
    68461 ttcaccctgc ttacttttat attccgccag aatcggtaca aatgcctggg aaaatgcccc
    68521 ttcggcaaag atacggcgta acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt
    68581 tgccatccct gcgccaaaga ttctggcgac aattgcgtct cgtgcgaagc caagcacacg
    68641 cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt aataaattca tcggtgttct
    68701 aatccagacc cacgggcaaa acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac
    68761 ctggattatg gtgaattgct accgccagat gttacagggt ttattcactc atcgcatcgc
    68821 gccagatctt gtcaacgata cgttgcgcca gcacggcctg ttcgccggcg gtttgcggaa
    68881 ctgtctgatt ttgcacacat tcaatgaagt gccgcgcaca gccgacaaat ccacgttgtt
    68941 caagagtgct ctgccaacca ggaatcggtt tatgcactac gccctgcccg cgctcctcac
    69001 gccattcgcg catatccgta atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt
    69061 cacgctgact tccggcacgg cgatgcatac aggtggtgat ctgcaaagga ccggccgaaa
    69121 aatggtgctc ggcaaacagc atttcgccag cgtcgttagt cagtagcgta ccgccatcca
    69181 gagaggcttt gccgcccgac aaccacagcg cggtatccac cacatgcaga taatcatcca
    69241 gcaacgtgaa ataaagatcg tgtggcccga cgctattgct acgatgttta tccattctta
    69301 gcgaagctgc ggtagcgagt tgcgttttta actcaccgta gagtggtgcg aaacgacggt
    69361 taaaaccgac catcaacgtc agttttttac gcgccgccag ttccaccagc cgttcagcat
    69421 cgcgcagatt ttctgccagc ggtttatcga cacagacatg tacacccgca ttgagtaacg
    69481 tactgaccac gtcaaagtgg ctggcggtgc tggaatgcac aaaaaccgca tcgcaactgg
    69541 cggcaaggct ggataacgaa tcggcataag gaatgcgcca gctttcacaa attggcaggg
    69601 ctttcgcgcg cgtaggcgac caggctcctt gtaacgtcca gtcagacgct gccgccagca
    69661 ccggtaacca cgctttttgc gcaatgccac ctaatcccac tacgccgata cgtaattttt
    69721 tcactttaat ctcccagatg ggccagcaac gaatcaagac gctgtttcag ttctgccact
    69781 tcgatttcca gggcttcgac gcgggcctgt aaatcaccat caaccgcgtt cgacatatcc
    69841 gtcaccgccg gctgatcttc aacctcacca ctgaaaagat gcatgtagcg gctttcgcgt
    69901 ttacccggtt cgcgggccag acgcaccaca aaaggaccat cttcgcgatt tgccagttgt
    69961 tccagcgtcg actccacttc cgccatatcg ctgaattcat acattcgcgc ggcgcggctg
    70021 cgcagttcac ctggcgtctg ggcaccacgc aataacaacg tggtgattaa cgccacttcc
    70081 gctgcgctca gtttcagatc gccaaattct gaattacaaa aacgttgctc atatttggtg
    70141 acccgattac caaaaccgct cactgtgcgt agataatgac gtttgaccag attatccagc
    70201 tgttcctgca cttcggattc actcagattc atgaccggtt cacggttcgt tttctgatta
    70261 caggccgtga ctacaccatt gactgagagc gggtattgct ccggcgtggt cacctgcttt
    70321 tccagcaagc aaccaatcac gcgagcttca agggcagtaa gttgatattt catgagattc
    70381 tccttagcgg ccgggcgtcc agtctggggt cgttaatgcc gtcagtacgt gatcgcgcca
    70441 ttgtccatca atcaacagat agtctttcgc atagccttct ttttcaaaac ccagtcgcgc
    70501 cagtaaatca ccgctgcgtt tattgtgcgg catataatta gccataatgc gatgaatatg
    70561 ttgggtgcgc tgcatataac gaatggctgc ggtcagggct tcaaacatga gtcctttgcc
    70621 ctgccatttt tgcccaatcg aataaccgag atagcaggca tgaaaagagc cgcgaacaac
    70681 attggaaaaa ttggcaacgc caataatctc tttttcgtcc gggtcgaata aaccaaagta
    70741 gaaagctgaa ccttgtttat gaaattcgtt aatcatcccc agcctggcct gccagcctga
    70801 tggataacag tggctttcgt cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc
    70861 tgcgtaataa tccgcaagac gccaggcatc acgatcatgc accagacgca cgaccagtcg
    70921 gtctgtggtt aagcgcactt ttggcacgtt actgcgatag ccaaacattc tatacctact
    70981 ccttcccgta acgtctttaa tacgctgtaa gtattactat acctgcgcaa ccagaccctg
    71041 tgaaaagtcc aaaatgccat tttctgcatt attcacatat ttgataaaaa ctctctatca
    71101 aagcgacgtt ttgataaaaa aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa
    71161 tgcagaatag aaagtgctca tcttttattt cccgggaggg gaaatgtccc gcgtgtcgca
    71221 ggcgaggaac ctgggtaaat atttcctgct catcgataat atgctggtcg tgctggggtt
    71281 ctttgttgtc ttcccgctga tctctatccg cttcgttgat caaatgggct gggccgccgt
    71341 catggtcggt attgctctcg gtctacgcca gtttattcag caaggtctgg gtattttcgg
    71401 cggcgcaatt gccgaccgct ttggtgccaa accgatgatt gttaccggta tgctgatgcg
    71461 cgctgccgga ttcgccacaa tgggtatcgc ccacgaaccg tggctattgt ggttttcatg
    71521 cctgctctcg ggactcggcg gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa
    71581 actaatccgt ccacaacagc gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc
    71641 cggtgcagtc attggcgcat tgttggggag ctggctgttg caatacgact ttcgcctggt
    71701 ctgcgccaca ggggccgttt tgtttgtgct gtgtgcagcg ttcaatgcgt ggttgttacc
    71761 agcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg
    71821 tgacaagcgt tttgtcacct atgtcctgac gctggcgggt tactacatgc tggctgtaca
    71881 agtgatgctg atgctgccaa ttatggtcaa cgacgtggct ggcgcgccct ctgccgttaa
    71941 atggatgtat gccattgaag cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg
    72001 gagtgaaaag cattttcgtc tggaacaccg gttgatggct gggctgttga taatgtcatt
    72061 aagcatgatg ccggtgggca tggtcagcgg cctgcaacaa cttttcaccc tgatttgtct
    72121 gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc
    72181 ggacgcaaga gctcgcggca gctatatggg gtgtagccgt ctgggtctgg cgattggcgg
    72241 cgctattggt tatatcggtg gcggctggct gtttgacctg ggtaaatcgg cgcaccagcc
    72301 agagcttccg tggatgatgc tgggcattat tggcatcttc actttccttg cgctgggttg
    72361 gcagtttagc cagaaacgcg ccgcgcgtcg tttgcttgaa cgcgacgcct gatttgatac
    72421 tcccccctga ctcctccata ctgaaagtga actgacagcc aaatggagga gtcacgtgaa
    72481 gctatacatt tacgatcact gcccttactg cctcaaagcc cgcatgattt tcggcctgaa
    72541 aaatatcccc gtcgaattac atgttctgct caacgacgac gcagaaacac ccacccggat
    72601 ggtcggtcaa aaacaggttc ccattctgca aaaagatgac agccgctata tgccagaaag
    72661 catggacatc gttcactatg tcgataaact cgacggcaaa ccgttactga ccggcaaacg
    72721 ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc tacgccaaca aactgctgtt
    72781 gccgcgtttt gccaaatcgg catttgatga gttttctact cccgccgcac gcaaatattt
    72841 cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac ctgctggccc actctgacgg
    72901 tctgattaag aatatcagcg atgatttacg tgcgctggat aaactgatcg tcaaaccgaa
    72961 cgccgtgaat ggcgaacttt cagaagatga tattcagcta tttccgctac tacgtaatct
    73021 gacgctggta gccggaatca actggccaag ccgcgttgct gattaccgcg ataatatggc
    73081 gaaacagaca caaatcaatt tgttatcatc aatggcgatt taatcctgac ccgccgcggc
    73141 agcctgtatt cgcgggttgc cgccgttttc agttttttct gctggtgagt ttatcgctgg
    73201 tgacacatgc tgcgttgtgt caggatattg acgcagattt catgtcgagg actaccatga
    73261 acaagttttt atttgccgct gcattgatcg tcagtggcct gctcgttggc tgtaatcaac
    73321 tcacccaata caccatcacc gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt
    73381 tctcaaaaga tatcggttta cccggcgtgg ctgacgccca tattgttctg acaaacctga
    73441 ccagccaaat tggtcgcgaa gagccgaata aggttaccct aaccggagac gccaatctgg
    73501 acatgaactc cctgttcggt agccagaaag cgaccatgaa actgaagctg aaagcgctgc
    73561 ccgtgtttga taaagaaaaa ggcgcgatct tcctgaaaga gatggaagtg gtcgatgcga
    73621 cggtacaacc ggaaaaaatg caaacggtga tgcaaacgtt gcttccctat ttgaaccagg
    73681 cattacgcaa ttactttaac cagcaacctg cttacgtcct gcgcgaagat ggcagccagg
    73741 gcgaagcaat ggcgaaaaaa ctggcgaaag gcattgaagt gaagccaggc gaaattgtca
    73801 ttccatttac tgattaatca cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa
    73861 aacgtttccg cttatccttt gtgtccggca aaaacatccc ttcagccgga gcatagagat
    73921 taatgactgc accatcccag gtattaaaga tccgccgccc agacgactgg caccttcacc
    73981 tccgcgatgg cgacatgtta aaaactgtcg tgccgtatac cagcgaaatt tatggacggg
    74041 ctatcgtaat gcccaatctg gctccgcccg tgaccacagt tgaggctgcc gtggcgtatc
    74101 gccaacgtat tcttgatgcc gtacctgccg ggcacaattt caccccattg atgacctgtt
    74161 atttaacaga ttcgctggat cctaatgagc tggagcgcgg atttaacgaa ggcgtgttca
    74221 ccgctgcaaa actttatccg gcaaacgcaa ccactaactc cagccacggc gtgacgtcaa
    74281 ttgacgcaat catgccggta cttgagcgca tggaaaaaat cggtatgccg ctactggtgc
    74341 atggtgaagt gacacatgca gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa
    74401 gcgtgatgga acctctgcgc cagcgcctga ctgcgctgaa agtcgttttt gagcacatca
    74461 ccaccaaaga tgctgccgac tatgtccgtg acggaaatga acggctggct gccaccatca
    74521 ctccgcagca tctgatgttt aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc
    74581 tgtattgttt acccatcctc aaacgcaata ttcaccaaca ggcattgcgt gaactggtcg
    74641 ccagcggttt taatcgagta ttcctcggta cggattctgc gccacatgca cgtcatcgca
    74701 aagagagcag ttgcggctgc gcgggctgct tcaacgcccc aaccgcgctg ggcagttacg
    74761 ctaccgtctt tgaagagatg aatgctctgc agcactttga agcattctgt tctgtaaacg
    74821 gcccgcagtt ctatggcttg ccggtcaacg acacattcat cgaactggta cgtgaagagc
    74881 aacaggttgc tgaaagcatc gcactgactg atgacaccct ggtgccattc ctcgccgggg
    74941 aaacggtacg ctggtccgtt aaacaataaa aattgtagct tcctgttgtc attaggttat
    75001 tttacctgta taaataacca gtatattcaa cagggggcta ttatgcgaat tgaagtcacc
    75061 atagcgaaaa cttctccatt gccagctggg gctattgacg ccctggctgg cgaactttcc
    75121 cgccgtattc agtatgcgtt tcctgataat gaaggccacg tatcggtacg ttatgccgca
    75181 gcgaataatt tatcggttat tggcgcaaca aaagaagata aacagcgcat tagcgaaatt
    75241 ctccaggaaa cgtgggaaag cg
//
LOCUS       DAPGWD010000003        73031 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPGWD010000003 DAPGWD010000000
VERSION     DAPGWD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.003"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(12..1016)
                     /gene="trpS"
                     /locus_tag="RHX88_000149"
     CDS             complement(12..1016)
                     /gene="trpS"
                     /locus_tag="RHX88_000149"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDV2228885.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(1009..1767)
                     /gene="gph"
                     /locus_tag="RHX88_000150"
     CDS             complement(1009..1767)
                     /gene="gph"
                     /locus_tag="RHX88_000150"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDV2228886.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(1760..2437)
                     /gene="rpe"
                     /locus_tag="RHX88_000151"
     CDS             complement(1760..2437)
                     /gene="rpe"
                     /locus_tag="RHX88_000151"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDV2228887.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(2455..3291)
                     /gene="dam"
                     /locus_tag="RHX88_000152"
     CDS             complement(2455..3291)
                     /gene="dam"
                     /locus_tag="RHX88_000152"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV2228888.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(3398..4684)
                     /gene="damX"
                     /locus_tag="RHX88_000153"
     CDS             complement(3398..4684)
                     /gene="damX"
                     /locus_tag="RHX88_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDV2228889.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPATKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYGNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(4776..5864)
                     /gene="aroB"
                     /locus_tag="RHX88_000154"
     CDS             complement(4776..5864)
                     /gene="aroB"
                     /locus_tag="RHX88_000154"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDV2228890.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(5921..6442)
                     /gene="aroK"
                     /locus_tag="RHX88_000155"
     CDS             complement(5921..6442)
                     /gene="aroK"
                     /locus_tag="RHX88_000155"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDV2228891.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(6843..8081)
                     /gene="hofQ"
                     /locus_tag="RHX88_000156"
     CDS             complement(6843..8081)
                     /gene="hofQ"
                     /locus_tag="RHX88_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDV2228892.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(7993..8397)
                     /gene="hofP"
                     /locus_tag="RHX88_000157"
     CDS             complement(7993..8397)
                     /gene="hofP"
                     /locus_tag="RHX88_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDV2228893.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(8387..8827)
                     /gene="hofO"
                     /locus_tag="RHX88_000158"
     CDS             complement(8387..8827)
                     /gene="hofO"
                     /locus_tag="RHX88_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDV2228894.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(8811..9350)
                     /gene="hofN"
                     /locus_tag="RHX88_000159"
     CDS             complement(8811..9350)
                     /gene="hofN"
                     /locus_tag="RHX88_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDV2228895.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(9350..10129)
                     /gene="hofM"
                     /locus_tag="RHX88_000160"
     CDS             complement(9350..10129)
                     /gene="hofM"
                     /locus_tag="RHX88_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDV2228896.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            10249..12801
                     /gene="mrcA"
                     /locus_tag="RHX88_000161"
     CDS             10249..12801
                     /gene="mrcA"
                     /locus_tag="RHX88_000161"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDV2228897.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(12966..13526)
                     /gene="nudE"
                     /locus_tag="RHX88_000162"
     CDS             complement(12966..13526)
                     /gene="nudE"
                     /locus_tag="RHX88_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDV2228898.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            13846..15981
                     /gene="igaA"
                     /locus_tag="RHX88_000163"
     CDS             13846..15981
                     /gene="igaA"
                     /locus_tag="RHX88_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDV2228899.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            16046..16714
                     /gene="yrfG"
                     /locus_tag="RHX88_000164"
     CDS             16046..16714
                     /gene="yrfG"
                     /locus_tag="RHX88_000164"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDV2228900.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            16725..17126
                     /gene="hslR"
                     /locus_tag="RHX88_000165"
     CDS             16725..17126
                     /gene="hslR"
                     /locus_tag="RHX88_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDV2228901.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            17151..18029
                     /gene="hslO"
                     /locus_tag="RHX88_000166"
     CDS             17151..18029
                     /gene="hslO"
                     /locus_tag="RHX88_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDV2228902.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(18164..19888)
                     /locus_tag="RHX88_000167"
     CDS             complement(18164..19888)
                     /locus_tag="RHX88_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDV2228903.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQVSEKV
                     LADNVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTKPKAWRDLTVDGE
                     TLEINLSK"
     gene            20267..21889
                     /gene="pckA"
                     /locus_tag="RHX88_000168"
     CDS             20267..21889
                     /gene="pckA"
                     /locus_tag="RHX88_000168"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDV2228904.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            22005..22322
                     /locus_tag="RHX88_000169"
     CDS             22005..22322
                     /locus_tag="RHX88_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2228905.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            22381..22677
                     /locus_tag="RHX88_000170"
     CDS             22381..22677
                     /locus_tag="RHX88_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2228906.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKSI"
     gene            complement(22707..24059)
                     /gene="envZ"
                     /locus_tag="RHX88_000171"
     CDS             complement(22707..24059)
                     /gene="envZ"
                     /locus_tag="RHX88_000171"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDV2228907.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTIKEG
                     "
     gene            complement(24056..24775)
                     /gene="ompR"
                     /locus_tag="RHX88_000172"
     CDS             complement(24056..24775)
                     /gene="ompR"
                     /locus_tag="RHX88_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDV2228908.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            25003..25479
                     /gene="greB"
                     /locus_tag="RHX88_000173"
     CDS             25003..25479
                     /gene="greB"
                     /locus_tag="RHX88_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDV2228909.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            25576..27897
                     /locus_tag="RHX88_000174"
     CDS             25576..27897
                     /locus_tag="RHX88_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDV2228910.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            28335..28562
                     /gene="feoA"
                     /locus_tag="RHX88_000175"
     CDS             28335..28562
                     /gene="feoA"
                     /locus_tag="RHX88_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDV2228911.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            28579..30900
                     /gene="feoB"
                     /locus_tag="RHX88_000176"
     CDS             28579..30900
                     /gene="feoB"
                     /locus_tag="RHX88_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDV2228912.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            30900..31136
                     /gene="feoC"
                     /locus_tag="RHX88_000177"
     CDS             30900..31136
                     /gene="feoC"
                     /locus_tag="RHX88_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDV2228913.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            31339..32217
                     /gene="rpnA"
                     /locus_tag="RHX88_000178"
     CDS             31339..32217
                     /gene="rpnA"
                     /locus_tag="RHX88_000178"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDV2228914.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(32246..33016)
                     /gene="bioH"
                     /locus_tag="RHX88_000179"
     CDS             complement(32246..33016)
                     /gene="bioH"
                     /locus_tag="RHX88_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDV2228915.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            33054..33737
                     /gene="gntX"
                     /locus_tag="RHX88_000180"
     CDS             33054..33737
                     /gene="gntX"
                     /locus_tag="RHX88_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDV2228916.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            33796..34371
                     /gene="nfuA"
                     /locus_tag="RHX88_000181"
     CDS             33796..34371
                     /gene="nfuA"
                     /locus_tag="RHX88_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDV2228917.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            34732..36048
                     /gene="gntT"
                     /locus_tag="RHX88_000182"
     CDS             34732..36048
                     /gene="gntT"
                     /locus_tag="RHX88_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV2228918.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(36093..38177)
                     /gene="malQ"
                     /locus_tag="RHX88_000183"
     CDS             complement(36093..38177)
                     /gene="malQ"
                     /locus_tag="RHX88_000183"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDV2228919.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAIRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNEDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(38187..40580)
                     /gene="malP"
                     /locus_tag="RHX88_000184"
     CDS             complement(38187..40580)
                     /gene="malP"
                     /locus_tag="RHX88_000184"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDV2228920.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            41192..43897
                     /gene="malT"
                     /locus_tag="RHX88_000185"
     CDS             41192..43897
                     /gene="malT"
                     /locus_tag="RHX88_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDV2228921.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREDTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(43940..44956)
                     /gene="rtcA"
                     /locus_tag="RHX88_000186"
     CDS             complement(43940..44956)
                     /gene="rtcA"
                     /locus_tag="RHX88_000186"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDV2228922.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMCGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(44960..46186)
                     /gene="rtcB"
                     /locus_tag="RHX88_000187"
     CDS             complement(44960..46186)
                     /gene="rtcB"
                     /locus_tag="RHX88_000187"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDV2228923.1"
                     /translation="MNYELLPTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGTTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQQQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            46375..47973
                     /gene="rtcR"
                     /locus_tag="RHX88_000188"
     CDS             46375..47973
                     /gene="rtcR"
                     /locus_tag="RHX88_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDV2228924.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(47955..48713)
                     /locus_tag="RHX88_000189"
     CDS             complement(47955..48713)
                     /locus_tag="RHX88_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDV2228925.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(48730..49560)
                     /gene="glpG"
                     /locus_tag="RHX88_000190"
     CDS             complement(48730..49560)
                     /gene="glpG"
                     /locus_tag="RHX88_000190"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDV2228926.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(49605..49931)
                     /gene="glpE"
                     /locus_tag="RHX88_000191"
     CDS             complement(49605..49931)
                     /gene="glpE"
                     /locus_tag="RHX88_000191"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDV2228927.1"
                     /translation="MDQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            50121..51626
                     /gene="glpD"
                     /locus_tag="RHX88_000192"
     CDS             50121..51626
                     /gene="glpD"
                     /locus_tag="RHX88_000192"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDV2228928.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(51680..52284)
                     /locus_tag="RHX88_000193"
                     /pseudo
     CDS             complement(51680..52284)
                     /locus_tag="RHX88_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(52287..53054)
                     /locus_tag="RHX88_000194"
     CDS             complement(52287..53054)
                     /locus_tag="RHX88_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2228929.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(53057..53767)
                     /locus_tag="RHX88_000195"
     CDS             complement(53057..53767)
                     /locus_tag="RHX88_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2228930.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(53782..55290)
                     /locus_tag="RHX88_000196"
     CDS             complement(53782..55290)
                     /locus_tag="RHX88_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV2228931.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(55415..57862)
                     /gene="glgP"
                     /locus_tag="RHX88_000197"
     CDS             complement(55415..57862)
                     /gene="glgP"
                     /locus_tag="RHX88_000197"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDV2228932.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDSQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(57881..59314)
                     /gene="glgA"
                     /locus_tag="RHX88_000198"
     CDS             complement(57881..59314)
                     /gene="glgA"
                     /locus_tag="RHX88_000198"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDV2228933.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(59314..60609)
                     /gene="glgC"
                     /locus_tag="RHX88_000199"
     CDS             complement(59314..60609)
                     /gene="glgC"
                     /locus_tag="RHX88_000199"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDV2228934.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(60627..62600)
                     /gene="glgX"
                     /locus_tag="RHX88_000200"
     CDS             complement(60627..62600)
                     /gene="glgX"
                     /locus_tag="RHX88_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDV2228935.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(62597..64783)
                     /gene="glgB"
                     /locus_tag="RHX88_000201"
     CDS             complement(62597..64783)
                     /gene="glgB"
                     /locus_tag="RHX88_000201"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDV2228936.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(65056..66159)
                     /gene="asd"
                     /locus_tag="RHX88_000202"
     CDS             complement(65056..66159)
                     /gene="asd"
                     /locus_tag="RHX88_000202"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV2228937.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            66351..66944
                     /gene="yhgN"
                     /locus_tag="RHX88_000203"
     CDS             66351..66944
                     /gene="yhgN"
                     /locus_tag="RHX88_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDV2228938.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(67001..68341)
                     /gene="gntU"
                     /locus_tag="RHX88_000204"
     CDS             complement(67001..68341)
                     /gene="gntU"
                     /locus_tag="RHX88_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV2228939.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(68345..68872)
                     /gene="gntK"
                     /locus_tag="RHX88_000205"
     CDS             complement(68345..68872)
                     /gene="gntK"
                     /locus_tag="RHX88_000205"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDV2228940.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(69011..70006)
                     /gene="gntR"
                     /locus_tag="RHX88_000206"
     CDS             complement(69011..70006)
                     /gene="gntR"
                     /locus_tag="RHX88_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDV2228941.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(70230..70925)
                     /gene="yhhW"
                     /locus_tag="RHX88_000207"
     CDS             complement(70230..70925)
                     /gene="yhhW"
                     /locus_tag="RHX88_000207"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDV2228942.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(71048..72085)
                     /locus_tag="RHX88_000208"
     CDS             complement(71048..72085)
                     /locus_tag="RHX88_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV2228943.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDELYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            72419..72907
                     /gene="yhhY"
                     /locus_tag="RHX88_000209"
     CDS             72419..72907
                     /gene="yhhY"
                     /locus_tag="RHX88_000209"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2228944.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        17725 a        19776 c        18340 g        17190 t
ORIGIN      
        1 gcgttttgat attacggctt cgccacaaaa ccaatcgctt cgtacaccgc tttcagcgta
       61 cgggaagcgt gtacactggc tttttctgcg ccgtctttca tcacctgttg caggaaggct
      121 tcatcgttgc ggaaacggtg atagcgttcc tgcaattcag tcagcatacc ggaaacggca
      181 tcagccactt cacctttcag atgaccatac atcttgcctt cgaactgttt ttccagttct
      241 gggatgctct ggcccgttac cgcagaaagg atatccagca ggttggaaac gcccgctttg
      301 ttctgcacat cgtagcgaac taccggcggc tcgtcggagt cagtgaccgc gcgtttgatt
      361 ttcttcacta ccgatttcgg atcttccagc aggccgataa cgttattgcg gttatcgtca
      421 gacttggaca tcttcttggt cggctccagc agcgacatta cgcgcgcgcc agatttcgga
      481 ataaacggct ccggcacctt aaagatctcg ccatacagcg cgttgaaacg ctgggcgata
      541 tcgcgactca gttccaggtg ctgtttctgg tcttcaccca ccggtaccag attagtttga
      601 tacagcagga tgtccgcagc catcagcacc ggatagtcaa acagaccagc gttgatgttc
      661 tcggcataac gcgcagattt atctttaaac tgggtcatgc ggctcagttc gccgaagtag
      721 gtatagcagt tcagtgccca gcctaactgc gcatgttctg gtacgtggga ctgaacaaaa
      781 atggtgcttt tctcaggatc gataccacaa gccagataca aggccagcgt atccagcgtc
      841 gctttacgca gcttctgtgc atcctggcgc acggtgatcg cgtgttggtc aacgatacag
      901 taaatgcaat ggtagtcatc ctgcatgttt acccactgac gcagcgcacc catgtagtta
      961 ccaatggtca attcacctga gggctgtgcg ccactaaaaa cgatgggctt agtcattttt
     1021 cgattcctga ttttcgctat gcggaagccc taatgcgggc agaaggtcat ttatagactg
     1081 ataaattaca tcaggctggc tgagatcgat agcctcgccg tagttatatc cgtaggttaa
     1141 gccaactgat gggcaacctg ccgcttttgc cgcctgaata tcattgcgtg agtcgccgac
     1201 aaacagcatc tgttgtgggg caattcccat ccgctcagcc accagtaaca gcgggtccgg
     1261 atgcggtttt ttgttttgca catcatcgcc gccgataacc acgctaaagt atttggcaat
     1321 atctaaggct tcgagcagcg gcgcgacgaa cggcgtcggt ttgttggtga ccaggcctag
     1381 cggcaggcct ttagcctgca acgcgcccaa cgtatcggca acgtgcggga acaaaaacgt
     1441 cccctcttcg gcaacctcgc catagtagcg atcgaacagt ttacgcagaa tacgtacctg
     1501 ttcttctgcc ggaatgtcgt catcaacggg cggtttaccc attgttttac gctgagtcgc
     1561 acgttcctga cgcgcccagg tcaatgcgcg ctccatcaga acatctgcgc cgttaccaat
     1621 ccaggtaata acgcgttctt cacctgcgac gggcaactcc agcgcataca gcgccatatc
     1681 taccgcagca gcaagaccgg gagcactgtc gaccagcgta ccatcaagat caaaagcgac
     1741 gccgcgaata tcttcaaact tattcatgac ttacctttgc cagttcactg cgcatttcat
     1801 caatgacttt tttgtagtct ggctggtcga agattgccga accggcgacg aacatatccg
     1861 cgcccgccgc agcgatttcg ccaatgttgt tcaccttcac gccaccgtct acttccagac
     1921 gaatgtcaaa gccagactcg tcgatacgac gacgtacttc gcgcagttta tccagtgttt
     1981 gaggaatgaa cgactgaccg ccgaaaccag gattgacgga catcagcagg atcacatcca
     2041 gcttatccat cacgtaatcc agatagctca gcggtgtcgc cgggttaaat accagtcccg
     2101 ctttacagcc attttcttta atcagttgca gcgtgcggtc aacatgctcg gaggcttctg
     2161 gatgaaaggt aatgatgctg gcaccagcgg cagcgaaatc aggcacaatg cgatcgacag
     2221 gtttcaccat caggtgtacg tcgatagggg cggtaatgcc atagttacgc aaggatttca
     2281 gcaccattgg cccaatcgtc agattgggaa catagtggtt atccatgacg tcaaaatgca
     2341 cgacatcagc gccagctgcc agggcttttg cggtatcttc acccaggcgg gcaaaatcag
     2401 ccgacagaat tgagggggca atcaaatact gtttcatccg cttctccttg agaattattt
     2461 tttcgcgggt gaaacgactc ctggtttgta caaagccagc agttcgtcca cctttttacg
     2521 tgtgccgccg ttgctgctta tactgcgtcg aactttgacg acatgcaatt ttgcgcgctg
     2581 ataccactca cgcgttaaca tcgtatcgtg attggagatc agcactggaa tatggcgctc
     2641 aaccagacct tcggcgatct ccgccagatg cgcttgttgt tcaagcgtaa aactgtttgt
     2701 gtgatacgcc gtaaagttgg cggtcgcaga cagcggtgca taaggcggat cgcaatagac
     2761 gacggatgca tcatctgcgc gcgccatgct atcggcgtaa gactcacaat agaaaaaggc
     2821 attctgcgct ttttcagcga agtgatacaa ctctgcttcc gggaaatagg gttttttgta
     2881 gcggccaaac ggcacattaa actcaccgcg cagattgtaa cgacacaggc cgttgtaacc
     2941 gtagcggttc aaatataaaa acagtaccgc ccgacggaac ggatcctggc ttttgttgaa
     3001 ctcttcgcgg aactgatagt aaacctcggc gcaatttgtt tcgggaacaa acagctcgcg
     3061 tgcggcctgt acgtactcat cagtacgcat cttcacaatg ttatagagac tgatcaggtc
     3121 gctattgata tcggcaagga tataacgaga aaagtcggtg ttgagaaaca ccgacccggc
     3181 acctacaaaa ggctcaacca gacattcgcc cttgggcaaa tgccgtttaa tatcatcaag
     3241 caggggatac ttgccccctg cccacttcaa aaaagcgcga tttttcttca tgctgactaa
     3301 ctaattacac cttctccggc tgtggagaaa gctccgacag catccagcgc ttcaggcaac
     3361 tcccgcaacg ataaaccatt gcgggagata accttgatta cttcagatcg gcctgtacct
     3421 gacgcagcgg tttcgcccac gggtttttgg cctggacatc tgctggcaat gtagataccg
     3481 cttttttcgc ctcttctttc gaagcgtaca cgccagaaac caggacatac cacggctgac
     3541 cattacgcgt cgtttcatag acaacgtagt ttttcagatt ctctttcttc gcccaaccgt
     3601 tcaggttgcc gtagttagag gaactgctca gctgcagagt gtaatggctg gacggtgccg
     3661 atttcaacga accaacatta cctgcggtct tccctccagc ggctggtgtt gccgtggttt
     3721 gcgccgggga tgccgtctgt actggagccg tagtcgccgt ctcttttgtt gctggcgtag
     3781 aagtcgcagc cggtgccttc gtgctcgcca caggggcagc tggttcagta cgcttcggcg
     3841 tctgtgctac cggcttcggc tccgttttca cggttgcttg cggttttttc ggttcaatca
     3901 ctgcctgctg gcgagctgga cgcgtagtgg ccggacgttc agcggtttgc gttttcgccg
     3961 tttcacggga tgcattgcca ttgcgaacag gcgcgacagt cgcgggttcg gtcggcaatg
     4021 tggaattgac cgccacattg ttcaactgtt gctgattttg tggttgggtc agcgcattgt
     4081 tcaggtcacc ctgcacttca acacgttgtt gaccatccgt tgccgccggg gtttgccctt
     4141 gagtcggcgt agaagagatc ggcggcagag aaacatcctg ctgagtattt tccgcagacg
     4201 tggttcccgg cgctggctgc acgccattgg cctgatcggt cgcattgcca gcaagatcaa
     4261 tactcttctc gccagacgcg gtttgatcgc tggaagaggt cgagggggct tttagcgcag
     4321 aaccgatacc gatgatcaac agcagtagaa ccagaatgcc gacgcccatc atcatatact
     4381 gacgagaagc gggtttactg gctgcttttt tgcgcttacg cggacgacgc tctacgcgct
     4441 cttcatccac ggtttcatct tcggattcgt caatttcttc ttcgatttcc ggttcctcat
     4501 tgcgctcttt ttgcgcacga gtcggacggc gatcgtcagt gtcatcaagt tcaatatcat
     4561 caaaattgat ctgcggttcg ccacgttcag tacgctcaga acgttcagaa gattgacgag
     4621 aacgaccagt acgacgatcg ctgggatcgg gtttcagctc gtcttctggt ttgaattcat
     4681 ccatttaaca ccccactaaa aggttaatgc ttaccacgtt gcaattaacc tgaagctaat
     4741 agaccgcttg ataagcggcc tgacctttct tgttgttacg ctgattgaca atcggcaatg
     4801 gcgttaagaa caagctcgtg cgaaacgccg ctgcgaactt cactcttacc aattgccaac
     4861 ggaagaatta agcgcatctc tcccgcaagg actttcttgt cacgcagcat atgcggtaaa
     4921 tacgcctgcg cggacatttc gcgcggccca ttgaccggta acccagcccg cgtgagcagg
     4981 gttataatac gctgcgtttc ggcagaacta aactgcccga gacgttccga cgtccgcgcc
     5041 gccatcacca tacccgcagc gaccgcttca ccatgtaacc aattgccata ccccatttca
     5101 gcttcaatgg catgaccaaa ggtgtgtccc agattcagta aagcacgtaa cccggtttcg
     5161 cgctcgtcgg cggcgacaac ttctgccttc agttcacaac aacggcgaat acagtacgcc
     5221 attgccggac cgtccagacg caacaacgca tccagattct cttccagcca gttaaaaaac
     5281 gcaccgtcaa gaataatgcc gtatttgatg acttctgcca gccccgacgc taactcacgc
     5341 gggggaagcg ttttcagaca gtcgagatcc accaccactg aagcaggctg gtagaacgcg
     5401 ccaatcatgt ttttaccgag gggatggttg accgcagttt tgccgccaac ggaggaatcg
     5461 acctgcgaca gtaacgtcgt cgggacttga atgaaacgaa caccgcgctg ataactcgcc
     5521 gccgcgaagc cggtcagatc gcccactacg ccgccgccaa gcgccaccag cgtagtatcg
     5581 cgaccatgcg gcttttgtaa caacgccgta aagacggtat cgagtacagc caggctttta
     5641 tactgctcgc cgtcagggag gataacgcta tcgacgttaa cacccgcctg ttcaagtacg
     5701 ccgcggacct tatcgagata cagaggagcc agggtttcgt tggtgaccaa catgacctgc
     5761 tcgcccgatt tcagcggtaa gaatgaagct ggttcattaa acaaaccaga tgcgatggta
     5821 attgggtaac tacgttcccc gagagtaacg acaatcctct ccataacgcg acatccacct
     5881 taattactgt acccgcagac gagtgtatat aaagccagaa ttagttgctt tccagcatgt
     5941 gaataatctg gtttgcaacc actttagcgc tttgatcatc agtacgaatg gtcacgtcgg
     6001 caatctcttc atacagcgga ttgcgttcat tggccaacgc ttccagaact tcacgcggcg
     6061 gtgtttcaac gtgcagcaac gggcgttttt tatcacgctg cgtgcgtgca agttgctttt
     6121 cgatggtcgt ttcaagataa acgacaacgc cacgagcgga aagacggtta cgcgtttcac
     6181 gggatttcac agagccgccg ccagtagcca gcacaatacc ctgtttctcg gtcaactcat
     6241 tgatgacctt ttcttcgcga tcgcggaagc cttcttcgcc ttctaaatcg aaaacccagc
     6301 ccacatcagc tccggttcgt ttctcaatct cttgatcgga atcgtaaaat tccatattga
     6361 gttgttgagc taactggcgc ccaatagtgc tttttccggc acccataggc ccaaccagaa
     6421 agatattgcg tttctctgcc attttttcgg tactactaag actattcgtt aatgataaac
     6481 ccgcttcgct cagagagcgc cgcaggacat gaactgaaac ctcataagat attgcgagag
     6541 tcagactgaa aattatctca atactccagc gggtttggca actgaataaa tcacccatcc
     6601 ctctgcatat ctggtcgctg caagcgcgct gccttgctac caccgctctg gcgataaatc
     6661 accgggtaag attagcgtaa aaaagacagc aaaatgccgc ctgaatgata aatcatcatc
     6721 atggaacgca ggcagcgaag agcaccaact ctcaaatcgg tactccttgt atgctaaata
     6781 cctgcgcgcg tcaaatagat gaaacacgtt cagcgtaaaa acattaccgc ttttacggct
     6841 gtttactcac tggaaaccag tcgtggcgtg ataaacacca ctaactcgcg tcgttcatct
     6901 tcttttccgt catgacgaaa taattgcccg aaccagggaa tgtcgccaag caacggtacg
     6961 ctatcctgac ccgatttatt tttacgggta aaaatgccgc ccagcgccaa cgtttctccg
     7021 cttttgacct cgacctgcgt ttcgatctcc tgcttatcaa tcgccagcac ttcgccatcg
     7081 gcctgctgta gcacctgccc cggaacgttc tggctgatgt gtaatttcag ccggatgcga
     7141 cctttttgta acaccgtggg cgtgacctcc atccccagga cggcctcttt aaattccacc
     7201 gacgtcgcgc cactttcccc gctggaaacc tgatatggaa tttcgctccc ctgtttaatg
     7261 ctggcaggct gaagatgtga ggccagcaga cgcggactgg cgataatatc cagctgctgt
     7321 ttttgttcga gcgcggaaag ctcaagatcc agcaagcgtc cgttgatgcg cccaatgtta
     7381 aaaccgacat gcgttgtcgc cgtcgctacg gagaggtcgc taccaagcgt ggtgacttgc
     7441 ccaacgccac cagcgtgttg cgcatcggcc agcgtccatt tcacgcctaa ctcacgcaaa
     7501 cttttttcat taatggtgac aatatgcgcc gacagctcaa cctgcccgac cggcagatcc
     7561 atttgcgcta cccactgttc aagcgcgctt aacgccgttt tgttatctcg tagcaaaaga
     7621 cgattggtgc gtttatcgac ggtcatactc cctttggcac tcagtagctt ctcccccgct
     7681 ttcgccagtt ctcccgcgtc ggcgtattgc agggttatac tgcgattttc cagcggcaga
     7741 tttgcctgcg cccgcgcctg ctccgcctcc tggcgggcga tattgttatt ctgccaggca
     7801 atggaatgca ctgagagaat gttgccttcc tgccgcgtta tcagtccggc gcttttcact
     7861 acagtttgta gtgcctgctt ccagggaaca tctgttagat gtaacgacac cgtaccgctg
     7921 acgtctggcg acacgaccag gttcaacttc tcctgttcag ccagcgcctg caacacctga
     7981 gctaccggaa cgtcatccac catcagcgtc actttttgcg gctttgccgc ctgtacgccg
     8041 ggtatcagca tcaacagtag tgcggctatc cattgcttca tttgtatctc cttgccgttg
     8101 ccacaaccat tgtggcggtt cgcagtttgt cccggtaccc agcgttagta cgtctggcgt
     8161 cagctgtaaa attgtccagc cgttttccag cacatcgttt tgctgcaccc gtcgccattt
     8221 cttttgcccg tcttttatta caccgatgat gcgctcgcct cgccctacca tcccctgata
     8281 gcgccactgg ctaagttcgc taatccggca tagatcttcc ggcggtttaa aagggtcacg
     8341 cataccggtt aaaaggcaca atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg
     8401 gcgtctccag ttgtagcgtg aacaaaagat catcaccttc cacgcttaac gaaaaacggc
     8461 tcacgctgac gttgcgctct gccagccgtg taaatgccga cggcactgct tcccacagcg
     8521 ttttcaacgc caactcgcct ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg
     8581 ataactgaaa atccagtggc gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt
     8641 ctaccaggcg atacagtgcg gcccactgct ggtgatgact tgcccgtagt cgaattaatg
     8701 cgtcgcgctc ttcatggtgt gtcgatgata gaaaaataag cgtaactaac atcagcaacc
     8761 agactgccca gcaaagctgg cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca
     8821 tgttcatcgc taaccttcct tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc
     8881 tgctgcgtgg ctccccgctg attgagatga aaagacgcat cctggcggag tgacgtttct
     8941 agtgcgttta acgcggtaat gcttgttgtc agccccttga tctccagcgt tccctgctgc
     9001 cagcttatcg ttgtcagcca ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca
     9061 gattgccagt cgcgggtaaa ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac
     9121 tgctgttgct ccagcaaacg tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc
     9181 gcctgaagca aaactgcgtc tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc
     9241 gttatcccga cggccagcag cagaggcgca acgaacatca gcaaccagaa acgcagaaaa
     9301 gcggtccggc gttgctgtcg ccagggcaaa aaattaattg gcgggttcat cagtacgcct
     9361 tcccaagcgc cagccccagc gcgatggcaa agtctccacc tggcggcggt agcggcggct
     9421 gacgaacaga aacggcctcc cagggatcaa atccgccttc gccacatatc gcgacgcttt
     9481 cgggatcaac ggataacgct gccgccagct ccttcgcgct agtcatcccc accgccagtt
     9541 tgcgccccca gcgacagcgt gtcgcccaca gccactgttc gttatcacgc caggccagac
     9601 attgctgatg agaaggtaaa aaaggcagga attgctgtaa tgcactggca tccggggtga
     9661 tcgcgctcac atgaacacgc aacctttctg ccagagtaag tagcgttgcc agctctttgc
     9721 tttgcgcggc agtcacgtta taggcggggc tgagtgagtc ttcgctgtaa tcgaagcgca
     9781 gggagtccgg atccatatcc agctcgcggg ccatcgtccc tgacagccag gccgtttgct
     9841 cccgctcacc aagggacatc gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg
     9901 gaaacgccaa cataatgtga tgacgctgcg gcagttcgcg actccacggt aacaacgttt
     9961 tagccagctg ctgcgcatca acaatccgcc catctttgat aatgtcgttc tccagcggca
    10021 accgccacca gcgttgcaaa aagcattctt ttgctccccg cacgatcgca accgctaccg
    10081 cttcttgctg ttgtaaatgc aaaccaattt gccagatctt aaatgccatt gtgatgatct
    10141 ccttatcacc cgtcactctg acgggtatat caatgcgtct ggcttgcctt tatactaccg
    10201 cgcgtttgtt tataaactgc ccaaatgaaa ctaaatggga aatttccagt gaagttcgta
    10261 aagtattttt tgatccttgc agtctgttgc attctgctgg gagcaggctc gatttatggc
    10321 ctataccgct acatcgagcc acaactgccg gatgtggcga cattaaaaga tgttcgcctg
    10381 caaattccga tgcagattta cagcgccgat ggcgagctga ttgctcaata cggtgagaaa
    10441 cgtcgtattc cggttacgtt ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg
    10501 acggaagaca gccgcttcta cgagcatcac ggcgttgacc cggtggggat cttccgtgcg
    10561 gcaagtgtgg cgctgttctc cggccacgcg tcacaagggg cgagtaccat tacccagcag
    10621 ctggcgagaa atttcttcct cagtccagaa cgcacgctga tgcgtaagat taaggaagtc
    10681 ttcctcgcga ttcgcattga acagttgctg acgaaagacg agatcctcga gctttatctg
    10741 aacaagattt acctcggtta ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc
    10801 ggtaaaacgg tcgaccaact gacgctgaac gaaatggctg tgatcgccgg gctgccgaaa
    10861 gcgccttcca cctttaatcc gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc
    10921 gtactgtcgc ggatgctgga tgaagggtat atcacccaac aacagttcga tcagacacgc
    10981 actgaggcga ttaacgctaa ctatcacgcg ccggagattg ctttctctgc gccgtacctg
    11041 agcgaaatgg tgcgccagga gatgtataac cgttatggcg aaagtgccta tgaagacggt
    11101 tatcgcattt acaccaccat cacccgcaaa gtgcagcagg ccgcacagca ggcggtacgt
    11161 aataacgtgc tggactacga catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg
    11221 aaagtgggcg agtcggcgtg ggataacaac aagattaccg atacgctgaa ggcgctgcca
    11281 acctatggtc cgctgctgcc tgccgcagtc accagcgcca atcctcagca agcgacggcg
    11341 atgctggcgg acgggtcgac cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct
    11401 taccgttcgg atactcagca aggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg
    11461 ggtcagcaaa tctgggttcg tcaggttggc gatgcatggt ggctggcaca agtgccggaa
    11521 gtgaactcgg cgctggtgtc gatcaatccg caaaacggtg ccgttatggc gctggtcggt
    11581 ggctttgatt tcaatcagag caagtttaac cgcgccaccc aggcactgcg tcaggtgggt
    11641 tccaacatca aaccgttcct ctacaccgcg gcgatggata aaggtctgac gctggcaagt
    11701 atgttgaacg atgtgccaat ttctcgctgg gatgcaggtg ccggttctga ctggcagccg
    11761 aagaactcac caccgcagta tgctggtcca attcgcttac gtcaggggct gggtcagtcg
    11821 aaaaacgtgg tgatggtacg cgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat
    11881 ctgcaacgct tcggcttccc ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt
    11941 tcagcgtcct tcaccccaat gcaggtggcg cgcggctacg cggtcatggc gaacggtggc
    12001 ttcctggtgg acccgtggtt tatcagcaaa attgaaaacg atcagggcgg cgtgattttc
    12061 gaagcgaaac cgaaagtagc ctgcccggaa tgcgatattc cggtgattta cggtgatacg
    12121 cagaaatcga acgtgctgga aaataacgat gttgaagatg tcgctatctc ccgcgagcag
    12181 cagaatgttt ctgtaccaat gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag
    12241 actggcgcgc aggagtacgc accgcacgtc atcaacactc cgctggcatt cctgattaag
    12301 agtgctttga acaccaatat ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca
    12361 ggtcgtgatt tgcagcgtcg cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa
    12421 gatgcgtggt tctcgggtta cggtccgggc gttgtgacct cggtctggat tggctttgat
    12481 gatcaccgtc gtaatctcgg tcatacaacg gcttccggag cgattaaaga tcagatctca
    12541 ggttacgaag gcggtgccaa gagtgcccag cctgcatggg acgcttatat gaaagccgtt
    12601 cttgaaggtg tgccggagca gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc
    12661 gatcgcagca ccgggcagtt agctaatggt ggcaacagcc gcgaagagta tttcatcgaa
    12721 ggtacgcagc cgacacaaca ggcggtgcac gaggtgggaa cgaccattat cgataatggt
    12781 gaggcacagg aattgttctg attaaaaagg cgcttcggcg ccttttcagt tggctgacaa
    12841 agtgcatttg tttatgccgg atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa
    12901 aatcaatata ttgcaggaac cccataagcc tgataagcgt tgtgcatcag gcaaacttca
    12961 cgcatttaca ctcgcccctg ccctttcaac cattcgcgca cgaggaacag cgcgctgaca
    13021 ttacgcgctt cgttgaagtc agggtcttcc agcaaatcca tcatatgcgc cagcggccag
    13081 cgcacctgtg gtagcggctc tggctcatcg ccttccagcg attccgggta gagatcttgc
    13141 gctaccacga tattcatttt gctggaaaaa taagacggtg ccatgctgag cttcttcaaa
    13201 aaagtcagat cgttcgcacc aaatccaacc tcttctttta gctcgcggtt agcggcttcg
    13261 tagacgcttt cacccggatc aattaatcct ttcgaaaaac ctaattcgta ggattcagtt
    13321 cccactgcgt attcgcggat caggatcagg tgatcgtcca caatcggcac aatcatcact
    13381 gcttcccggt tggttggacg cattcgttca taaacacgcc gcacgccatt gctgaactcc
    13441 agatccacgc tctcgacggt aaacagtcgg gaacgggcta cagtttcaac attcagaatg
    13501 gtgggttttt gtaatgattt gctcatcgtg ggatctatgc tgtgaaatca gcggttattg
    13561 tgcgatatcg gacacgcttt cggcaatgtg aattgcatgt tatttacatt tatgtaactt
    13621 aataaataat cgtcctcaaa tcaaattaaa agtcaatagg ttgaaataac tccaggaatt
    13681 tgctgatatt ccgccttcag agggtttgct atgatcagcg gttactgtga tgtgcttaat
    13741 gatgctcaag ttaaactcca cgcttgccga tagccaaccg cagaatcatg tattgtgtcc
    13801 ggtgcgactg accacgcctg acagactaag taagatgggg aaagcatgag caccattgtg
    13861 atttttttag ctgctttgct ggcctgctca ctacttgcgg gatggctgat aaaagtgcga
    13921 tccagacggc gtcagctgcc ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc
    13981 acacctgaag aacgtagcgc cgttgaaaat tatcttgaga gcctgacgca ggtattacag
    14041 gtgcctggcc caacgggagc cagcgcggca ccgatctctc tggcgctgaa tgccgaaagc
    14101 aacaacgtca tgatgctgac acacgctatc acgcgttacg gcatctctac cgacgatccg
    14161 aataaatggc gttactacct cgattcggta gaagtccacc tgcccccttt ctgggaacaa
    14221 tacatcaacg atgagaatac cgttgaactg attcataccg attcgctgcc gctggttatt
    14281 tcactcaacg gtcatacgct gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa
    14341 cctgttccat ccacgcaggc gtcgattcgc ggggaagaga gtgagcaaat cgagctactc
    14401 aatattcgca aagaaacgca tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa
    14461 gcgttgctga tcgtcgcctc cttcctgatg ttctttttct gcctgattac cccggatgta
    14521 tttgttccgt ggctggcagg cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta
    14581 ttcgcgcccc cggcaaaatc ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt
    14641 cgttggggat tgtttggcga aaacgatcag gaacagatca acaatatttc actcggtatt
    14701 atcgacctgg tctatcccgc acactggcag ccatacattg ctcaggatct cggtcaacaa
    14761 accgatattg atatctatct cgaccgccac gtagtgcgtc aggggcgata tctttcgctg
    14821 catgatgaag taaaaaactt tccgttacag cactggctgc gcagtacgat tatcgctgcg
    14881 ggctcgctgc tggtgctgtt tatgctgtta ttctggatcc cgctggatat gccgctgaaa
    14941 ttcactctct catggatgaa aggcgcgcag accattgaag ccaccagcgt aaaacaactg
    15001 gctgacgctg gcgtgcgggt aggcgataca ttgcgtatta gcggtacggg aatgtgtaat
    15061 attcgcactt ccggaacctg gagcgcgaaa accaattcac cttttttacc gtttgactgc
    15121 tcgcagatca tctggaatga cgcccgctca ttaccattac cagaatctga actggtcaac
    15181 aaagcgacgg cattgactga agcagttaat cgccagctgc accctaaacc ggaagatgaa
    15241 tctcgcgtca gtgcctcatt acgttcagcg attcaaaaat ccggcatggt attgcttgat
    15301 gattttggcg acattgtact gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg
    15361 cgactgaaaa atgcgctggt caatctcggc aacagtaaag actgggacgc gctggtaaaa
    15421 cgcgccaacg ccgggaagct cgatggcgtg aatgtgttat tacgcccggt gagtgcggaa
    15481 tcgctggata acctggtggc aacctccacc gcgccgttta tcacgcatga aacggcgcga
    15541 gcggcacaat cactaaacag tccggccccc ggcggattcc tgattgtcag cgacgaaggc
    15601 agcgattttg ttgatcagcc ctggccttcg gcatcacttt acgactaccc gccgcaagaa
    15661 cagtggaacg ctttccagaa actggcacaa atgctgatgc atacgccgtt taacgccgaa
    15721 ggtatcgtca caaaaatctt cactgacgcc aatggtacgc agcatattgg ccttcatccg
    15781 atcccggatc gttccggcct gtggcgctat ctcagcacca cattgctgct actgacgatg
    15841 ctgggtagcg ccatttacaa tggcgtacag gcctggcgtc gttaccagcg tcatcgcact
    15901 cgcatgatgg agattcaggc ctattatgaa agctgcctga acccgcaact gatcacccct
    15961 tcagaaagcc ttatcgaata acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa
    16021 ttgcttcact ctggagattt ccctcatgca tatcaacatt gcctggcagg acgtagatac
    16081 cgttctgctg gatatggacg gcacgttgct cgacctcgcc ttcgataact atttctggca
    16141 aaagctggtg cctgaaacat ggggcgcgaa aaacggggtt acgccacagg aagcgatgga
    16201 atatatgcgc cagcaatatc acgacgtaca gcatacgcta aactggtact gtcttgatta
    16261 ctggagtgag caactgggtc tggatatctg tgcgatgacc accgagatgg gaccgcgcgc
    16321 cgtactgcgt gaagatacca ttccgtttct tgaggcactg aaagccagcg gtaagcagcg
    16381 aattttgctc accaatgctc atccgcacaa cctggcggta aaacttgagc atacgggtct
    16441 ggacgcacac cttgatttat tactttccac ccacacattt ggttatccga aagaggatca
    16501 gcggttatgg catgcggtgg ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat
    16561 tgatgacagc gaagcgattc tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg
    16621 cgtgactaat cctgattccg ggattgccga gaaacagtat caacgccatc cgtcactgaa
    16681 tgactaccgc cgcctgatcc cctcgctaat gtgaaggaga cgccatgaaa gaaaaacctg
    16741 ctgttgaggt tcgactggat aaatggctat gggctgcccg tttttataaa acccgcgcgc
    16801 tggcccgtga aatgattgaa ggcggtaagg tgcattacaa cgggcagcgc agcaagccga
    16861 gcaaaatcgt cgagctgaat gccacgctca ctctgcgcca gggaaatgac gaacgcacgg
    16921 tgattgtaaa ggcgattact gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg
    16981 aagagactgc ggaaagtgta gagaaacgcg aaaaaatggc gctggcacgt aaacttaatg
    17041 ccttaaccat gccgcacccg gaccgacgcc cggacaaaaa agagcgccgc gacctgttac
    17101 gatttaaaca cggcgacagt gaataactgt cacctgcaag agagatgatt atgccgcaac
    17161 atgaccaatt acatcgctat ctgtttgaaa actttgccgt gcgcggcgaa ctggtaaccg
    17221 tttcggaaac cctgcaacag atccttgaga atcacgatta tccgcagccc gttaaaaacg
    17281 tgctggcaga actgctggtt gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg
    17341 atatcaccgt acagctgcaa ggcgacggtc cgatgaatct ggcggttatt aacggtaaca
    17401 ataaccagca aatgcgcggt gtggcgcgcg tgcagggcga aattccagaa aatgccgacc
    17461 tgaaaacgct ggtcggcaat ggttacgtgg tgatcaccat taccccgagc gaaggcgaac
    17521 gctatcaggg cgtggttggt ctggaaggtg ataccctggc ggcctgcctg gaagattact
    17581 ttatgcgttc tgaacagctg ccgacgcgcc tgtttattcg caccggcgac gtagacggca
    17641 aaccggctgc aggcggtatg ttgttgcagg taatgcctgc gcaaaatgcc cagcaggacg
    17701 actttgacca cctggcgacg ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac
    17761 cggcaaacga agtgttgtgg cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc
    17821 aggatgtgga gttcaaatgc acctgctcgc gtgaacgttg cgccgatgca ctgaaaacgc
    17881 tgcctgatga agaagtcgat agcatcctgg cggaagatgg cgaaattgac atgcattgtg
    17941 attactgcgg taaccactat ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg
    18001 cgtctccggc agatccgcaa gttcattaat gagtatgtcc ggcagagagg gctctgccgg
    18061 attttacgac gtaatggcct gatgtgctgc gcttaccagg cctatgaaat aaatcgcaaa
    18121 ttattgttgg tcagatacag cgtcgtatcc gataaaagcg ttgtcactta cttaagttta
    18181 tttccagcgt ttcaccatct accgtcagat cgcgccacgc tttaggcttc gtccccagtt
    18241 ttccatcctt cgcagctgtg agtaattttt ctttcgttat ttcgttcgga agtaaactca
    18301 tatcaagcgc gtcccattct tttctgtctg agtcaaagcc atagacaatg actctgtcat
    18361 cattaaaagc aaacaggatc cgctccgcct gaccatcact attcagatct tgctcgacca
    18421 ggacgcaggc gtctttttca atacaggtca tcacgttata gcgatcctgg attaaggccg
    18481 accagaatgt cgcatccggt ttaacagaac caggggcaat taacacatta tcggctaata
    18541 ctttttccga tacctgttgc tgaagatgtt gctctccatc taacgccatc agcagatccc
    18601 gcgcgcgttt cgggtctttc ataaacccgg catcgctttt cagcgactca agcgcagcac
    18661 gtccatagcg accgctctgc tcgagcatgt agatagttac ctggtctgac gtgtttttgc
    18721 cgctctgata acgcgccata tggctgttca cgctaatgcg catactgtcc agcaccggcg
    18781 aattaagaag caccagtatc accaacacca ataacgaaac cgcaaggttc actttgccct
    18841 gaagaacaag gggattttgc ccattacgcc agacgatact gacaaaatac cccagcgacc
    18901 acaccagtaa caccagcacc gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact
    18961 gagcgacccg cagccataac gcccaggcgg cgacgaacac atacagcgga gcaaccagca
    19021 aagcggtttt aatcaggcaa cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat
    19081 cgcggacaat agccattaag atcaattgca aaaaggccag cgttaacagc aacccggcgg
    19141 cggagatgtg gcgagaaatc gcgctcaggc ccgtaaacgg cagagtgatg ataaacatca
    19201 gggttagcaa tgatactaac ggcagcaacc ccgtggcgat taacgtgaac aacttttgaa
    19261 tagagtcgat taaacgtgac tgtgtgcgcg cgagaatcac cgccagcgcg gtaaccagac
    19321 ctaacgtgag atacataaac cagtcggttg caaaaaagag cgtattgaaa aacttgatgc
    19381 caacaagttt aaacaactca ccccacagta aaagcaccag ccacgtcagg ccattggcga
    19441 gaaaaatcac cagtaatata agtacgttat gccatactga ctggtaaaaa tagcgatagc
    19501 ggctactgtc attgcggatg cgaaggcttt gttgtatcca cggtagcaac aacattgcca
    19561 tcaacaacag atagcagcca aaatcccaaa tcgccttttc agctctccag gggttcatgc
    19621 catcagtttg ccacttcagc caaccgctca tccctgtcgt ggcaataaac accagcgcca
    19681 gccatcccca aaggcgcttc tgtttaaaag agatcacgga gaagagtaaa acggatgaaa
    19741 acgcaacggt cgcaggcacg ccataaacaa tccaactgtg attttttccg gcaagccagg
    19801 cgatcaacag gtagcaaacc agcccctgaa gtaatccggt agcaatcatc ccccagcgtg
    19861 ttgccggtga aagttcaacg ttatccatgt tggttatcca gaatcaaaag gtgggttaat
    19921 tatcgcatcc gggcagtagt attttgcttt tttcagaaaa taatcaaaaa aagttagcgt
    19981 ggtgaatcga tactttaccg attgaatttg catcaatttc attaaggaat gcgattccac
    20041 tcacaatatt cccgccatat aaaccaagat ttaacctttt gagaacattt tccacaccta
    20101 aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag gaatcacgga gttttttgtc
    20161 aaatatgaat ttctccagat acgtaaatct atgagccttg tcgcggttaa cacccccaaa
    20221 aagactttac tattcaggca atacatattg gctaaggagc agtgaaatgc gcgttaacaa
    20281 tggtttgacc ccgcaagaac tcgaggctta tggtatcagt gacgtacatg atatcgttta
    20341 caacccaagc tacgacctgc tgtatcagga agagctcgat ccgagcctga caggttatga
    20401 gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat accgggatct tcaccggtcg
    20461 ttcaccaaaa gataagtata tcgtccgtga cgataccact cgcgatactt tctggtgggc
    20521 agacaaaggc aaaggtaaga acgacaacaa acctctctct ccggaaacct ggcaacatct
    20581 gaaaggcctg gtgaccaggc agctttccgg caaacgtctg ttcgttgtcg acgccttctg
    20641 tggtgcgaac ccggatactc gtctttccgt ccgtttcatc accgaagtgg cctggcaggc
    20701 gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa gagctggcag gtttcaaacc
    20761 agactttatc gttatgaacg gcgcgaagtg cactaacccg cagtggaaag aacagggtct
    20821 gaattccgaa aacttcgtgg cgtttaacct gaccgagcgc atgcagctga ttggcggcac
    20881 ctggtacggc ggcgaaatga agaaagggat gttctcgatg atgaactacc tgctgccgct
    20941 gaaaggtatc gcttctatgc actgctccgc caacgttggc gagaaaggcg atgttgcggt
    21001 gttcttcggc ctttccggca ccggtaaaac caccctttcc accgacccga aacgtcgcct
    21061 gattggcgat gacgaacacg gctgggacga tgacggcgtg tttaacttcg aaggcggctg
    21121 ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct gaaatctaca acgctatccg
    21181 tcgtgatgcg ttgctggaaa acgtcaccgt gcgtgaagat ggcactatcg actttgatga
    21241 tggttcaaaa accgagaaca cccgcgtttc ttatccgatc tatcacatcg ataacattgt
    21301 taagccggtt tccaaagcgg gccacgcgac taaggttatc ttcctgactg ctgatgcttt
    21361 cggcgtgttg ccgccggttt ctcgcctgac tgccgatcaa acccagtatc acttcctctc
    21421 tggcttcacc gccaaactgg ccggtactga gcgtggcatc accgaaccga cgccaacctt
    21481 ctccgcttgc ttcggcgcgg cgttcctgtc gctgcaccca actcagtacg cagaagtgct
    21541 ggtaaaacgt atgcaagcgg caggtgcgca ggcgtatctg gttaacaccg gctggaacgg
    21601 cactggaaaa cgtatctcga ttaaagatac ccgcgctatt atcgacgcca tcctcaacgg
    21661 ttcgctggat aacgcagaaa ctttcactct gccgatgttt aacctggcga tcccaaccga
    21721 actgccgggc gtagacacga agattctcga tccgcgtaac acctacgctt ccccggaaca
    21781 gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc gacaacttcg ataaatacac
    21841 cgacacccct gcgggtgccg cgctggtagc ggctggtccg aagctgtaat cagctgatat
    21901 ctcattgacc ccttatccaa acggataagg gtttttttat atctgattag ctaccaatat
    21961 gtacacggta cacttataat tatcaataga ccgcaggatg cattatgttc acctttattg
    22021 aactacaagg atttagcaaa cgccgccagt tattgctacc tgatgatgag ttccgggctt
    22081 ttcaggaact actcattcaa gatcccactg ccggtgacat tatcgcaggc acaggcagtt
    22141 ttagaaaaat tcgctggaac cgttctggaa tggggaaacg gggtggcatt cggataattt
    22201 attacaacgt aacacgcaat ggccgaattt atctggcgtt gatttatcct aaaaatgaac
    22261 aggatgattt aacagaagaa caaagaaagg cattgaaact attgtccgag aagctgcttt
    22321 aatgttgaag ctccacaaat tccaccatgg ccgaaatgcc aacggaggca ttgaaaacag
    22381 atgaaagatg aattatttgc tgatcttctt gccagcgcag aagaaatggt acgcatcgaa
    22441 aagggtgagg aaacgcctga accagagcac gtccatactt ttagcgaaat cgatgtaaaa
    22501 gcgatccgtg aagcaacggg tttacgtcag caagattttg ctgtcgccgt aggggtaagt
    22561 tatgacctgg tcaaaagctg ggaaaccaaa cgccgtcaac ctaccggagc acctcgaaaa
    22621 ttgctcctgc ttttgcaagc gaaccctttc attatcaatc aattgaaatc aatataaaac
    22681 aggaggcacc ttcgcctccc gtttatttac ccttctttta tcgtgccctg cgcccgcgtt
    22741 accggaacag gcagccaggc acgaatggaa agcccgcccc gctcgctggt gccaagctcc
    22801 agcatcccgt tatggttatc cacgatgcgc tgcacaattg ccagccctag ccccgtgccg
    22861 ctaatggtgc gcgcactgtc gccgcggaca aatggctgga acaggtgctt acgctgttcc
    22921 ggtgcaattc ccggaccgtc atcttccacc tggaaccagg cgcgattcgg ctccgttccg
    22981 ctgctgactt tgatccagcc attgccataa cgggcggcgt tgaccaccat attcgccacc
    23041 gcgcgtttga tcgacagcgg gtgcattttc acttcaatgc tgccggggta aagcgcggtt
    23101 tcaatttccc gctcatagcc actttcggca gcaatcacct caccgagtac tgcattaaga
    23161 tccgccattt ccatcggcat ctcctgcccg gtgcgcaggt agtcgataaa ctgctcaatg
    23221 atggcgttgc actcttcgat atctttattg atcgattctg ccagatagcc atcctgctcg
    23281 ctcatcatct cagtcgccag gcgaatacgc gtcagcggcg tgcgcaagtc gtgacttacc
    23341 cccgccatca gcagcgtgcg gtcatccgcc agttgcttaa caccagccgc catatggtta
    23401 aaggcacggg taacggaacg cacctccgaa gcgccatact cacgcagcgg cggcggaata
    23461 atccctttac caacctgcaa ggctgcgtgt tcgagatcga ccaacggtcg gttctggata
    23521 cgaataaaca gccacgcccc gcctatcgcc aatagcataa tcgccagcgt atagcggaac
    23581 agcggagaga aatcgccctg atgaatttcg gtcagcggca cgcgtaccca gatattgggc
    23641 gacagccagg ttttcagcca gacgacaggc gaacttttgt tgacctcaac gcgcacttcc
    23701 gtcgggccgc ccagttgctg tgccatctga tggcttaaga attcatagtg ttgcgcccaa
    23761 cgcagacctg cctcttcggc agcctcgttg gagtagagag agatccccag ctcacggtag
    23821 atctcccgac ggaaagcggg aggcacaacc aactgcgtgc cgtcctccag ttgcagtttg
    23881 tcggtcatca acatacgcac ttcgtacgcg aggactttat taaactgctg gaggctcggc
    23941 aaaatcgcga agttcagcac caccagataa gtcgtcacca ggctggcgaa cagcaaggtg
    24001 acgatgagca ataacgtacg ggcaaatgaa cttcgtggcg agaagcgcaa tcgcctcatg
    24061 ctttagagcc gtccggtaca aagacgtagc ccaggcccca aacggtctga atgtaacgcg
    24121 gatgcgctgg atcttcttct accatgcggc gcagacgcga aatctgcacg tcgatggagc
    24181 gttccattgc ggaatattca cgaccacggg caaggttcat cagcttatcg cgggagagcg
    24241 gctcacgcgg atggctgacc agtgccttca gtaccgcaaa ctcaccgctg gtgagcggca
    24301 tcggctcatc ttcgcggaac atttcgcgcg taccgaggtt aagtttgaac ttaccgaaag
    24361 caattaccgc ctcttcctgt gacggtgcgc ctggcagttc gttcgcctga cgacgcagca
    24421 ccgcacgaat acgagccagc agttcacgcg ggttaaacgg ttttggaatg tagtcgtcag
    24481 cgccaatctc caggcctacg atacggtcca cttcttcccc tttcgccgtc accataatga
    24541 tcggcatcgg gttgctctga ctacgaagac gtcggcaaat cgacaagcca tcttcaccag
    24601 gtaacattaa atccagtacc ataagatgga aagattcacg agtcagcagg cgatccatct
    24661 gttctgcatt agcgacgctt cgaacctgga agccttgttc ggtgagataa cgttccagca
    24721 gcgcacgcag gcgcatgtcg tcatcgacca ccagaatctt gtagttctct tgcattgttt
    24781 gtactcccaa aggttcgcaa caatttgtaa gcgtgtattc ttaaaaaagc tcacgttcgt
    24841 caccagctaa atctggtatg aatttcagcc taaattgtta caaagcatat taaacagcag
    24901 cttaagtata caatttattc ggcgaaacat tattgattct gttgatatga tcacgttata
    24961 cccaatgtgc gcattatcaa acagacaaag ggaatcaacg agatgaaaac gcccctggtt
    25021 acccgggaag ggtatgaaaa actcaaacaa gagcttaatt atctctggcg tgaagaacgc
    25081 ccggaggtca caaaaaaggt gacctgggcc gcaagtctgg gcgaccgcag cgaaaatgct
    25141 gactatcagt ataataaaaa gcgtctgcgt gaaatcgacc gtcgcgtgcg ctatctcact
    25201 aaatgcatgg aaaatctcaa aatcgtcgat tactcccctc agcaggaagg caaagtcttt
    25261 tttggcgcgt gggtggagat tgaaaacgac gatggcgtga ctcaccgttt ccgtattgtc
    25321 ggctacgatg aaatttttgg ccgtaaagat tacatctcta tcgattcccc gatggcccgc
    25381 gcattgctga aaaaagaagt cggcgatctg gctgtggtga atactcctgc cggggaagcg
    25441 agctggtatg ttaatgctat cgagtacgtg aaaccgtaag gaagagtctt aacctcctgc
    25501 cgatggctgg catttttgcc agccagtccg tataactatc ccctgatttt tgatccgaaa
    25561 agatgaactc aaaccatgat gaatgattcg ttctgccgca ttattgcggg tgaaattcag
    25621 gcgcgcccgg aacaggttga cgctgccgtt cgcctgcttg acgaagggaa taccgtgccg
    25681 tttatcgcac gttatcgtaa ggaaatcacc ggcggtctgg atgacacgca gctgcgtaat
    25741 ctggaaacgc gtctgagcta tctgcgcgag ctggaagaga gacgtcaggc gatcctcaag
    25801 tccatttccg agcaaggcaa actcaccgat gatctggcga aggccatcaa cgccacccta
    25861 agcaaaaccg aactcgaaga cctctacctg ccctacaaac ctaaacgccg cacccgcggg
    25921 caaatcgcca ttgaagcagg gcttgagccg ttggctgacc tgctgtggag cgatccgtca
    25981 cacacgccag aagtcgccgc tgcacaatat gttgatgccg ataaaggcgt ggcagatacc
    26041 aaagccgcgc tggacggcgc gcgctatatc ctgatggaac gttttgccga agatgccgcg
    26101 ctgctggcga aagtgcgtga ttatctgtgg aagaacgcgc atctggtctc aaccgtggtg
    26161 agcggtaaag aagaggaagg ggcaaaattc cgcgactatt tcgatcatca cgaaccgttg
    26221 tccacggtgc cttctcaccg cgcgctggcg atgttccgtg ggcgtaacga aggcgtactc
    26281 cagctttcgc tgaatgccga tccgcagttc gatgagccgc ccaaagagag ctattgcgag
    26341 caaatcatca tggatcacct tggcctgcgc ctgaacaatg ccccggcgga tagctggcgc
    26401 aaaggcgtag tgagctggac ctggcgcatc aaggtgctga tgcatctgga aaccgaactg
    26461 atgggtaccg tgcgcgaacg cgcggaagat gaagcaatca acgtctttgc ccgtaacctg
    26521 cacgatctgc tgatggcggc ccctgccgga ctgcgtgcaa cgatgggcct cgatccgggt
    26581 ctgcgtactg gggtaaaagt ggcggtggtc gatgccactg gcaaactggt agcgaccgat
    26641 accatttacc cgcacaccgg acaagccgca aaagcagcga tgactgttgc tgcgctgtgt
    26701 gaaaagcata acgttgaact ggtggcgatc ggcaacggta cagcttcccg cgaaaccgaa
    26761 cgtttctatc tcgacgtgca gaagcagttc ccgaaagtga ccgcacagaa agtgatcgtc
    26821 agcgaagctg gcgcgtcggt ttactcggct tccgagctgg cagcgcagga gtttccggat
    26881 ctcgacgttt cgctgcgtgg cgcggtgtct atcgcccgcc gtttgcagga tccgctggcg
    26941 gagctggtga aaatcgatcc gaaatctatc ggcgtaggtc agtatcagca tgacgtcagc
    27001 cagacgcaac tggcccgcaa actggacgca gtagtagaag actgcgtaaa cgccgttggc
    27061 gtcgatctca acactgcttc tgttccgctg ttaacccgcg tggcgggcct gacgcgcatg
    27121 atggcgcaaa acatcgtggc ctggcgcgat gagaacggtc agttccagaa ccgtcagcaa
    27181 ctgctgaaag tgagccgtct ggggccgaaa gccttcgagc agtgcgcggg cttcttgcgc
    27241 attaaccatg gtgataaccc gctggacgcg tctaccgtcc acccggaagc ctatccggtg
    27301 gtggaacgca ttctggcagc aacacagcag gcactgaaag atctgatggg taacagcagc
    27361 gaactgcgta acctgaaagc gtctgacttt actgatgaaa aattcggtgt gccgacagta
    27421 actgacatca tcaaagagct ggaaaaaccg ggtcgcgatc cgcgtccgga atttaaaacc
    27481 gctcagtttg ccgatggcgt cgagacaatg aacgacctgc aaccgggtat gatcctcgaa
    27541 ggtgcggtga ccaacgtcac caactttggc gcgtttgtcg atatcggcgt gcatcaggac
    27601 ggcctggttc acatctcttc attgtcgaac aagtttgtag aagatccgca taccgtggtg
    27661 aaagcgggcg acattgtgaa ggtgaaagtg ctggaagtgg atcttcagcg taaacgtatc
    27721 gccctgacca tgcgtctgga cgagcagcct ggcgaaacca acgcccgtcg cggcggcggt
    27781 aatgaacgcc cgcaaaacaa ccgcccggca gccaaaccgc gcggacgtga agcgcagcct
    27841 gccggtaaca gcgcgatgat ggacgcgctg gcggcagcaa tgggcaaaaa acgttaaacg
    27901 ccctgattga cccctaaacc tgcgcgataa ggtgtccacg ccgcatctgg caacgaacgc
    27961 cttgtctgac ataaaagtgc cggagaatat ctccggcatt tttattccac agccaaactc
    28021 acaatatatt ccggcaacat ttatcacttc attaacaact gaaaccttaa ttaaacatta
    28081 gccagtcctg gtaattcgct attcgaatta tattttcgct gcgatataac cttgagccac
    28141 atcaacattg agtcagatta ttattcaaac caacattcgc acacatttta agtattgctg
    28201 atagaaacca ttctcattat cattgtgttg ttgattattt aatctctcct ttgttggcaa
    28261 atcatctggt ctcatgtcgc tgtcaaacgc cccatgaggt agttatccag ttaatgagaa
    28321 acaagtaggc acctatgcaa tacactccag atactgcgtg gaaaatcact ggcttttccc
    28381 gtgaaatcag cccggcatat cgccaaaaac tgctttctct tggcatgtta cctggctcct
    28441 cttttaatgt ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa acccgtcgtg
    28501 tgagcctggt attacgcaaa aaagatctgg ccttattaga agtggaagcg gtttcctgtt
    28561 aatacggtga taacaacaat gaaaaaatta accataggct taattggtaa tccaaattct
    28621 ggcaagacaa ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg taactgggct
    28681 ggcgttaccg tcgaacgtaa agaagggcaa ttctccacca ccgatcatca ggtcacgctg
    28741 gtggacctgc ccggcaccta ttctctgacc accatctcat cgcagacctc gctcgatgag
    28801 caaatcgcct gtcactacat tttgagtggc gacgccgacc tgctgattaa cgtggtggat
    28861 gcgtctaacc ttgagcgtaa cctgtacctg acgctacaac tgctggaact cggcattccc
    28921 tgcatcgtgg cactgaacat gctcgacatt gccgagaagc aaaatattcg tattgaaatt
    28981 gatgctctgt cggcgcgtct tggctgccca gtgatcccgc tggtttcaac ccgtggtcgc
    29041 ggtattgaag cgctcaagct ggcgattgat cgctataaag ctaacgagaa tgtggaactg
    29101 gtgcattacg cacagccgct actcaacgaa gcagattcac tggcaaaagt gatgccttcc
    29161 gacatcccgc tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg cgatatctac
    29221 agccgcgcct acgccggtga agcgtcgcag catctggatg ccgccctcgc ccgtctgcgt
    29281 aatgagatgg acgatccggc gctgcacatt gccgatgcgc gttaccagtg cattgctgcc
    29341 atctgtgatg tggtaagcaa caccctgacg gcagaaccca gccgtttcac cactgcggta
    29401 gataaaatcg tgctcaaccg tttcctcggt ctgccgattt tcctctttgt gatgtacctg
    29461 atgttcctgc tggctatcaa catcggcggg gcgttacagc cgctgtttga cgtcggctcc
    29521 gtggcgctat ttgtgcatgg tattcaatgg attggctaca cgctccactt cccggactgg
    29581 ctgactatct tcctcgccca gggcctgggt ggcggcatta acaccgtgct gccactggtg
    29641 ccgcagattg gcatgatgta cctgttcctc tccttccttg aggactccgg gtatatggcg
    29701 cgtgcggcgt ttgtgatgga ccgtctgatg caggcgctgg gcttgccggg gaaatccttt
    29761 gtgccgctga tcgtcggttt cggttgtaac gtaccgtcgg taatgggtgc acgtacgctt
    29821 gatgcaccgc gtgaacgtct gatgaccatc atgatggcac cgtttatgtc ctgcggcgcg
    29881 cgtctggcta tcttcgcagt attcgcggct gccttcttcg ggcagaacgg tgcgctggcg
    29941 gtcttctcgc tgtatatgct gggtattgtg atggcggtgc tgactggcct gatgctcaag
    30001 tacaccatca tgcgcggtga agcgacgccg tttgtcatgg agttgccggt ctatcatgta
    30061 ccacacgtta aaagcctgat tatccagacc tggcagcgtc tgaaaggctt cgttctgcgt
    30121 gctggtaaag tgatcatcat cgtcagcatt ttcctgagcg ctttcaacag cttctcgctg
    30181 agcgggaaaa tcgtcgataa catcaacgac tcggcgctgg cgtccgtcag ccgggtgatc
    30241 accccggtct tcaagccaat tggcgtgcat gaagataact ggcaggcaac ggttggcctg
    30301 tttacaggtg ccatggcgaa agaagtggta gtgggtacgc tcaacaccct ctacaccgca
    30361 gaaaatattc aggacgaaga gttcaatccg gcagagttta acctcggtga agagctgttc
    30421 agtgcgatag atgaaacctg gcagagcctg aaagacacct tcagccttag cgtactgatg
    30481 aaccccattg aagccagcaa aggcgacggc gaaatgggta ccggggcgat gggcgtgatg
    30541 gatcagaaat tcggtagcgc agcagcagct tacagctacc tgattttcgt cctgctgtat
    30601 gtaccatgta tctcggtgat gggagccatc gcccgtgaat caagccgtgg ctggatgggc
    30661 ttctccatcc tgtgggggct gaatatcgct tactcgctgg caacattgtt ctatcaggtc
    30721 gccagctaca gtcagcaccc aacttacagc ctggtgtgca ttctggcggt tatcctgttt
    30781 aacatcgtgg ttatcggtct gctgcgccgc gcgcgtagcc gggtggatat cgaactgctg
    30841 gcaacccgca agtcggtaag cagttgctgc gcagccagca ccaccggtga ttgccattaa
    30901 tggcttcact tattcaggtg cgcgatttgc tggcgttacg gggccgtatg gaagcggccc
    30961 agataagcca gacattgaac actccacagc caatgattaa cgccatgctg caacaactgg
    31021 aaagtatggg caaagccgtg cggattcagg aagaacctga cggctgcctc tctggcagtt
    31081 gtaaaagctg cccggaagga aaagcctgtc tgcgcgagtg gtgggcgctg cgttaacctt
    31141 actccatcgc ctgttttgga aagcccggta tgcgtctgca tccgggcttt tttgcgtgcg
    31201 gctttccata aaaatgcaac tcttgcagca cggcgtaagt tcctttgaaa gcatctcgca
    31261 gggatgaaaa ctcgctaata cacaggtgtg gagtggcgcg tagagtcgcg gcattcaaac
    31321 aacaggtgaa ggaacgccat gagcaaaaag cagagttcca ccccacacga tgcgctgttc
    31381 aaactctttt tacgccaacc ggacacggct cgtgattttc ttgcgtttca tttaccggca
    31441 cccattcacg cgctttgtga tatgaaaacc ctcaagctgg agtcgagcag ctttattgat
    31501 gacgatctgc gtgaaagcta ttccgatgtg ctgtggtcgg tgaaaacgga acaaggacca
    31561 ggatacatct attgtctgat tgaacatcaa agcacctcaa acaaactgat cgcatttcgc
    31621 atgatgcgtt acgctattgc cgcaatgcaa aatcaccttg atgctggata caaaacgttg
    31681 ccgatggtgg tgccattgtt gttttaccac ggtattgaaa gcccctatcc ctattcgctg
    31741 tgttggctgg attgtttcgc cgatcccaaa ctggcaaggc agctttatgc ctccgcattt
    31801 ccgctgattg atgtcaccgt catgcctgat gatgaaatca tgcagcaccg acgtatggcg
    31861 ctgctggagt taattcaaaa acatattcgt caacgcgatc tgatggggct ggtagagcaa
    31921 atggcctgct tattaagtag tggatacgct aatgacagac aaatcaaagg gctgtttaat
    31981 tacatactgc aaaccggcga cgcggtacgt tttaacgatt ttatcgacgg cgttgccgaa
    32041 cgttcaccga aacacaagga gagtttaatg acaattgcgg aaagattgcg gcaggagggg
    32101 gaacaatcca aagccctgca tatagccaaa ataatgcttg aatccggagt ccctcttaca
    32161 gacatcatgc gctttaccgg ggtgtcagaa gaagagttgg ctgcggcgag ccagtaaatt
    32221 tctgtctcgc catttcaaaa gttgcctaca ccctctgctt caacgccacc agcaggtgac
    32281 aaaactcgac cggatgcgaa ataaacggcg catgggccgc tttggcgaag atatatgatt
    32341 cgctgtgagg ccaaagttta tccagcatcg gcaccacttt gcgcggcacc agaccgtcga
    32401 gatagccata caatcgcaaa aacggcatgg acacgttttg cagcggctga cggagatcga
    32461 ccgttttcag gatttccagc ccgccattaa gcacgtcaac ctccggcatc ggtaacgcca
    32521 gaacggtttt cttcaacgcc cgcgcatcct ggcgcgccgt ttcagtcccc atggtttgta
    32581 acgccaggaa ccgctccact gtacgctgaa aatcatcact gagttgctgc tgaaatcccg
    32641 ccagcacgtc cggttttatc cccggccact cgtcacgagc actaaaacaa ggtgacgacg
    32701 ccacggtgac cagcgcctga acacgctcgg gatgggttaa aacaatttgg cttgccacca
    32761 gcccgcccag actccagcct aaccaaatgg ctttatcagg tgcctgttgc agcacggctt
    32821 cggccatatc agcaagtgac agcgcaccaa atccccggct acgcccgaag ccgggcaggt
    32881 caacaaggtg cagcgtaaaa tgcgagctaa gttcctcgtc aatgcaacgc cacacttcgg
    32941 cattcagtcc ccatccgtgc agcagcacaa gatgaacatt cccctgacct ttggtctgcc
    33001 accagatgtt attcatccgc tattgttctc ttttgactta caaggatgaa catatgctaa
    33061 cagtaccggg attatgctgg ctatgccgaa tgccactggc gttaggtcat tgggggattt
    33121 gttcggtctg ttcacgcgcc acccgcacag ataaaacgtt atgcccacaa tgtggattac
    33181 ccgccacaca ctcccatctt ccctgcggtc gctgcctgca aaaaccaccg ccctggcaaa
    33241 gactggtcac ggttgccgac tatgcgccgc cgttaagtcc gcttatccac cagcttaaat
    33301 tttcccggcg cagtgaaatc gccagcgccc tgtcacgtct gttactattg gaagtcttac
    33361 acgctcgtcg caccaccggg ttgcaattgc cggatcgcat catcagcgtt ccgttatggc
    33421 agcggcgtca ctggcgtcgg ggatttaatc agagcgattt gctgtgtcag ccgttatcac
    33481 gctggttgca ctgccaatgg gatagcgaag ccgtcacacg tacacgggcc actgcgaccc
    33541 agcattttct cagtgcccgg ctgcgcaagc gcaacctgaa aaatgccttt cgtcttgaat
    33601 tgcccgtgca aggtcgccat atggtgattg tggatgatgt cgttaccacc ggaagtaccg
    33661 tcgcagagat tgcgcagttg cttttacgca atggtgcggc gactgtccag gtctggtgcc
    33721 tttgtcgaac cttgtagagc ctcgatgatg ggcgtattat aaccaactaa aatagtcaac
    33781 tattaggcca ttactatgat ccgtatttcc gatgctgcac aagcgcactt tgccaaactg
    33841 ctggcaaatc aggaagaagg gacacaaatc cgcgtatttg tgattaaccc tggcacgcct
    33901 aacgctgaat gtggtgtttc ttattgtccg ccggatgccg tggaagccac cgacactgcc
    33961 ctgaaatttg acctgctgac cgcgtatgtt gatgagttaa gcgctcctta cctggaagat
    34021 gcagagatcg attttgttac cgaccagttg ggttctcagc taacactgaa agcgcctaac
    34081 gccaaaatgc gtaaagtggc agacgatgca ccactgatgg agcgcgtgga gtatatgctg
    34141 cagtcgcaga tcaacccaca gcttgctggt cacggtggcc gcgtttcact gatggaaatc
    34201 accgaagacg gttacgccat tctgcaattt ggcggcggct gtaacggttg ttccatggtc
    34261 gatgtgacgc tgaaagaagg gatcgagaag cagctgctaa acgaattccc ggagctgaaa
    34321 ggtgtgcgcg atctcaccga acaccagcgc ggcgaacact cctactacta agttatcccc
    34381 tcttttgtag attgcccgat gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt
    34441 gccagacgtt ggcacagcat tgctttggca aatccctccc ttcgtttccc cgcgataata
    34501 tgaccaacct ctcataattt aaatttaccc cgctctggtg attctcaaac gccagatgtt
    34561 acccgtatca ttcacatggg taccaaacat actcctgaca tctgactaca ataattagtt
    34621 ttagtgggga tcagtcgtgg tgccgcaata tctctgttcc cgattgggat aattagagtt
    34681 tgtcgtcaga aaattgacgt tacccataac aaatgaaagg ccaggtaaat catgccatta
    34741 gtcattgttg ctatcggtgt aatcttgttg ttgctcctga tgatccgctt caaaatgaac
    34801 ggcttcatcg ctctcgtcct cgtggcgctt gctgttggat taatgcaagg aatgccgctg
    34861 gataaagtta ttggctccat caaagccggt gtcggcggga cgctcggtag ccttgccctg
    34921 atcatgggtt ttggcgcaat gctgggcaaa atgctggcag actgcggtgg cgcacaacgt
    34981 atcgccacca cgctgattgc caaatttggt aaaaaacaca tccagtgggc ggtggtactg
    35041 accggtttta ccgttggttt tgccctgttc tatgaagtgg gctttgtgct gatgctgccg
    35101 ctggtgttta ccatcgcggc ttctgcgaat attccactgt tgtatgttgg tgtaccaatg
    35161 gcagctgcac tgtctgtgac ccacggcttc ctgccaccgc atccgggtcc gactgcgatt
    35221 gccaccattt tcaatgccga tatgggtaaa accctgctgt acggtactat tctggcaatc
    35281 ccgaccgtga ttctcgccgg tccggtttac gctcgcgtgt tgaaaggtat cgataagcca
    35341 attccggaag gtctctacag cgcgaaaacc ttcagcgaag aagagatgcc gagctttggc
    35401 gtcagcgtct ggacctctct ggtgccggta gtactgatgg cgatgcgtgc gattgccgaa
    35461 atgatcctgc cgaaaggtca cgctttcctg ccggtagcgg agttcctcgg tgacccggta
    35521 atggctacgc tgattgccgt gctgattgcc atgttcacct ttggtctgaa ccgtggtcgt
    35581 tcaatggatc agattaacga cacgctggtt tcttccatca aaatcattgc gatgatgctg
    35641 ttgatcatcg gtggtggcgg tgcgttcaag caggtgctgg tagacagcgg cgtggacaaa
    35701 tacattgctt ccatgatgca cgaaaccaac atttctccgc tgctgatggc ctggtcgatt
    35761 gctgccgtac tgcgtatcgc gctgggttcc gcaaccgttg cggcaatcac tgcgggtggt
    35821 atcgcggcac cgctgattgc aacgacgggt gttagcccgg agctgatggt tattgcggtg
    35881 ggttccggta gtgtgatttt ctctcatgtg aacgatccgg gcttctggct gttcaaagag
    35941 tactttaacc tgactatcgg cgagaccatc aaatcctggt cgatgctgga aaccattatc
    36001 tcggtgtgcg gtctggtagg ctgtctgctg ctgaatatgg tgatttgata acactgactg
    36061 ccggatgcat cgcatccggc atcaaccgca ctctacttct tcttcgctgc agctctgcgc
    36121 cgtctgtcca aatccttcag caacttgttc acgccatcat cggcaaacat cgactcaagc
    36181 gttgcggaaa gcttgcgtcg ccagttttta tactggtaac tggtgccagg aatattcacc
    36241 ggttcggcca tatccagcca gtcttccggc tgtagtccta acagagcact gttactgtcg
    36301 gcaatgtagc gctgcaaacc acggttcagc gtcggcgtca tcgacatcaa cgatgcctta
    36361 tgcccggcac gttttggcag acaaccatat ttatgcagtg catccagcag cccttgcttc
    36421 gccagttcgc gatcctgata cagaccgcgc agtaccactt catccggata cagccccagg
    36481 gttttgccca acgttagatc cccgctctcc cagtaaccgc gcagcgtagg caggtcatgt
    36541 gtcgccgcaa ccgccatcga ctgctccgga tacgctttcg gtgcacggaa cgtcttctcg
    36601 tggtcgtttt cgaaatagag cactttgtag gagtacacac cgctgctgcg cagcttaccg
    36661 acaatctcta ccggtacggt accgagatct tcaccaatca ccatacagcg atgacgttta
    36721 ctttcgagtg ccagaatcga gagcagatca tccaccggat agtgaacata cgcgccctga
    36781 tctgccgtct cgccatacgg tatccaccac aaacgcagca tcgacatcac atggtcaatt
    36841 cgtaatgcgc cgcagttttg catattggca cgcaacagct cgataaacgg ttcataggca
    36901 cgcgcggtga tgatatgcgg gtccattggc ggtaatcccc agttctgccc caacgggccg
    36961 aggatatccg gcggcgcgcc aaccgacgct ttcaggcaat atagttcacg gtcacaccag
    37021 gtttccgccc caccttccgc tacgccaacc gccagatcac gatacaagcc aatcggcatt
    37081 tcatagccct ggcttatctc ccagcaggcg gcaaactggc tgtaagccag ccactgcaac
    37141 cagagataaa aatcgacgtc attacgatgt tcttcgcaga actgacgcac ttctggtgaa
    37201 tcctcgttct gatacatctc tggccatgca ggccagcccc agcgcatttc gtcctctttc
    37261 acttgctggg catgtagcgc atcaaaggct gcctgccaga acaggctgtc gccctgctct
    37321 gcaacaaact ggcgaaacgc ggccatctgc tcatcatcac gttgcgcgaa acctttccac
    37381 gccattcgta atgctgtcat ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca
    37441 tcgcgcgcct gttgcagcgt ctgttgcgtg gtcggcaact gccaccaggc ctgagcctct
    37501 tcgctaagat ggaaatcttc aacggcgtta acgtcgatat aaatcacatt cagccaacgg
    37561 cgagaagacg ggctgtatgg gctggcactc tccggatttg ccggatagag cgcatgaatc
    37621 gggttcaggc cgatgaacga cccgccacgt attgccacat ccaccagcat cgctttgaga
    37681 tcgccaaaat ccccaatacc ccagtttttt tccgatcgca gcgtataaag ctgaacgcag
    37741 gcaccccaca gcttttgttt attcagcaac gcctgcggtt cgtaacagcg tttcggggcg
    37801 acaatcaccc ggcaatgcgc gcgctggtcg tcctgggtga gtgtcagcgt gtgataacct
    37861 tccggcagct tcgtcggtag attgaacgct ttgccccccg ttacatggcc tttgtactgt
    37921 gttccttctt cggtggtcag cagccagcta tattcgccgc tgccctccac caccatcggc
    37981 atttttttgc cgctggtata aaccatgaca ttcgggactg gcgttaccgc cactttcgtg
    38041 gcggtacgtt gatgcatcgc gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc
    38101 ggtttaccgt gggcattgat gtaattgggg ctaatccccg ccgccagcgc ggcattatcc
    38161 agacgtttgc tttccatcga gcttccttag cgttttgcct gccagatacg agcctgataa
    38221 tcgcgaatag agcgatccga gctaaacata ccgcagcggg cggtattgag gatcgccgcg
    38281 cgagtccagg cctcctggtc gcggtacagc acatccacct gcttttgtgc ctcaacatag
    38341 gctgcgaaat ccgccatcac cagatacgga tcgccgccct gtttgccgat actgtgcagc
    38401 atctggtcga aggcatgctt atcgccgtcg ctgtatttac cgctttccag ctctttcaat
    38461 actgcgtcca gcaccttatc tttcttccgc catttcaccg ggtcgtagcc tttggccaga
    38521 atagccttca cttgttccac ggtatgacca aaaataaaga tattttcttc accgactttc
    38581 tcggcgattt caacgttcgc accatccagc gtaccgacag taagcgcacc attgagcgcc
    38641 agtttcatat tgccggtacc ggaagcttct ttacctgcag tcgaaatttg ttcggagata
    38701 tccgccgccg ggatcagttt ttccgccgcc gaaacgcaat aatccggcag gaacaccacc
    38761 ttcaacttat cgccaaccag tggatcgttg ttgatcacgt cagccacttt gttgatcgca
    38821 aagataatat tcttcgccag gtagtagccc ggtgccgctt tcgcgccgaa gaggaagacg
    38881 cgcggtacgc gatcagcctg cgggttttca cgaatttctt tgtacaacgc cagaatatgc
    38941 agcagattca ggtgctggcg tttgtactca tgcagacgtt tgatctgaat atcgaaaatc
    39001 gcctgtggat tgatctcaat accggtacga actttcacaa actccgccag acggacttta
    39061 ttcgcctgct tgatctcgcg atattgctga cggaatttcg catcatcagc gaatttttcc
    39121 agattgatca gctgatcgag atcgttagcc cactcttttt gcagtgattt atccaacaga
    39181 gccgccagtg cagggttgca ctgtttgatc cagcgacgtg gggtaatacc gttggtgacg
    39241 ttatggaatt tgttcggcca tagctggtga tattccggga acagatcttt caccaccaga
    39301 tccgagtgca gcgccgcaac accgttcacc gcgaaaccgc caaccacaca caggttcgcc
    39361 atatgcactt gtttatcgtg caccaccgcc agtttggccc acactttttc atcgcccggc
    39421 caggttttct ccaccagcgt tttaaagcga gtattaattt cgttaataat ctgcatatgg
    39481 cgcggcagta agcctttcac cagtttcaca tcccagcgtt ccagcgcttc tggcatcagg
    39541 gtatggttgg tgtaagcgaa agttttgctg gtaatggccc aggcgtcatc ccagctcatc
    39601 tggtgctcat cgatcagcac gcgcagcagt tctggaatcg cgatagttgg gtgggtatcg
    39661 ttcagctgaa taacttcgta atccgccagt tcgtgcagtt tacgccccgc cagatgatgg
    39721 cgacgcaaaa tatccgctac cgaacaggca cactggaagt actgctgcat caagcgcagc
    39781 tttttaccgg cagtatggtt gtcgtttgga tagagaactt tggtcagttt ttccgcattg
    39841 atgccctgct gttcggcacg caggaaatca ccgtcgttaa atttagtcag atcaaacgga
    39901 tgcgcgtgcg tcgcctgcca cagacgcagc ggctgcgcca cgccattacg atagccgaca
    39961 acggggagat cccacgcttg accggtaatg gtaaactccg gctcccagcg tccgtctttc
    40021 gtcactttac cgccaatccc tacctgcaca tccagtgctt cgttgtggcg gaaccacggg
    40081 tagttactgc ggtgccagtc atccggcgct tcaacctgtt tgccatcgac aaaagactgg
    40141 cggaacaaac catattgata gttcagaccg taacccgtcg cagactgacc gacagttgcc
    40201 attgagtcga ggaagcacgc cgccagacgt cccagaccac cgttacccag cgccgggtcg
    40261 atctcttctt ccagcaggtc cgtcagattg atgtcataag ccttcaacga atcctgtaca
    40321 tcctgatacc agccgagatt caacaggttg ttgcccgtca ggcgaccaat caaaaactcc
    40381 attgagatgt agttaacatg tcgctgattc gccaccggct tggcgaatgg ctgagcacgc
    40441 agcatttcgg ccagtgcttc actcactgct agccaccact ggcgaggagt catttcagcc
    40501 gcagaattta agccataacg ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg
    40561 ttaaaaatag gttgtgacat aggagttcca cttttcttag attttcaaca caacgttatc
    40621 gctagtttgc caggctcgat gttgaccttc ctcatcctgc gggggattag gcagggagga
    40681 gttgcgggga tgagcaagga aatgtgatct caaccactta aagctagtgc aaaccacagg
    40741 attagcagca aatcaatgca atacagcgca gaaaatctgt atctaagtgc aaaaaatggc
    40801 cgttgcgtat tttcaaaaag cggaaggtaa ctctataaat taagtaaagg agtgaaacag
    40861 tttcataagt aaaatatcca gtgtgctcca tctcattctt aatagattta ttaagatcat
    40921 ctttttagat ggcactttca tcaggaatga agaagaaacc cttgcttaaa tgaatctgat
    40981 gaacataagg gaaaccagta ttcacgctgg atcagcgtcg ttttaggtga gttgttaata
    41041 aagatttgga attgtgacac agtgcaaatt cagacacata aaaaaacgtc atcgcttgca
    41101 ttagaaaggt ttctggccga ccttataacc attaattacg aagcgcaaaa aaaataatat
    41161 ttcctcattt tccacagtga agtgattaac tatgctgatt ccgtcaaaac taagtcgtcc
    41221 ggttcgactc gaccataccg tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa
    41281 caacttccgg ctggcgctga tcacgagtcc tgcgggctac ggaaagacca ccctcatttc
    41341 ccagtgggcg gcaggcaaaa acgatatcgg ctggtactcg ctggatgaag gtgataacca
    41401 gcaagagcgt ttcgccagct atctcattgc cgccgtgcag caggcaacca acggtcactg
    41461 tgcgatatgt gagacgatgg cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc
    41521 ccagcttttc attgagctgg cggaatggca tagcccactt tatctggtca tcgatgacta
    41581 tcatctgatc actaatccag tgatccacga gtcaatgcgc ttctttattc gccatcaacc
    41641 agaaaatctc accctggtgg tgttgtcacg caaccttccg caactgggca ttgccaatct
    41701 gcgtgttcgt gatcaactgc tggaaattgg cagtcagcaa ctggcattta cccatcagga
    41761 agcgaagcag ttttttgatt gccgtctgtc atcgccgatt gaagccgcag aaagcagtcg
    41821 gatttgcgat gacgtttccg gttgggcgac ggcactacag ctaatcgccc tctccgcccg
    41881 gcagaatacc cactcagccc ataagtcggc acgccgcctg gcgggaatca atgccagcca
    41941 tctttcggat tatctggtcg atgaggtttt ggataacgtc gatctcgcaa cgcgccattt
    42001 tctgttgaaa agcgccattt tgcgctcaat gaacgatgca ctcatcaccc gtgtgaccgg
    42061 cgaagaaaac gggcaaatgc gcctcgaaga gattgagcgt caggggctgt ttttacagcg
    42121 gatggatgat accggcgagt ggttctgcta tcacccgctg tttggcaact tcctgcgcca
    42181 gcgctgccag tgggaactgg cggcggagct gccggaaatc caccgtgccg ccgcagaaag
    42241 ctggatggcc cagggatttc ccagcgaagc gattcatcat gcgctggcgg caggcgatgc
    42301 gctgatgctg cgcgatattc tgcttaatca cgcctggagt ctgttcaacc atagcgaact
    42361 gtcgctgctg gaagagtcgc ttaaggccct gccgtgggac agcttgctgg aaaatccgca
    42421 gttggtgtta ttgcaggcgt ggctgatgca aagccaacat cgctacggcg aagttaacac
    42481 cctgctagcc cgtgctgaac atgaaatcaa ggacatcaga gaagacacca tgcacgcaga
    42541 atttaacgct ctgcgcgccc aggtggcgat taacgatggt aatccggatg aagcggaacg
    42601 gctggcaaaa ctggcactgg aagagctgcc gccgggctgg ttctatagcc gcattgtggc
    42661 aacctcggtg ctgggtgaag tgctgcactg caaaggcgaa ttgacccgct cactggcgct
    42721 aatgcagcaa accgaacaga tggcacgcca gcacgatgtc tggcactacg ctttgtggag
    42781 tttaatccag caaagtgaaa ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac
    42841 gcaggaaaaa gcattccagc tgatcaacga gcagcatctg gaacagctgc caatgcatga
    42901 gtttctggtg cgcattcgtg cgcagctgtt atgggcctgg gcgcggctgg atgaagccga
    42961 agcgtcggcg cgtagcggga ttgaagtctt gtcgtcttat cagccacagc aacagcttca
    43021 gtgcctggca atgttgattc aatgctcgct ggcccgtggt gatttagata acgcccgtag
    43081 ccagctgaac cgtctggaaa acctgctggg gaatggcaaa tatcacagcg actggatctc
    43141 taacgccaac aaagtccggg tgatttactg gcaaatgacc ggcgataaag ccgccgctgc
    43201 caactggttg cgtcatacgg ctaaaccaga gtttgcgaac aaccacttcc tgcaaggtca
    43261 atggcgcaac attgcccgtg cacaaatctt gctgggcgag tttgaaccgg cagaaattgt
    43321 tctcgaagaa ctcaatgaaa atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa
    43381 cctgttgctg cttaatcaac tgtactggca ggccggacgt aaaagtgacg cccagcgcgt
    43441 gttgctggac gcattaaaac tggcgaatcg caccggattt atcagtcatt ttgtcatcga
    43501 aggcgaagcg atggcgcaac aactgcgcca gctgattcaa ctcaatacgc tgccggaact
    43561 ggaacagcat cgcgcacagc gtattctgcg agaaatcaat caacatcatc ggcataaatt
    43621 cgcccatttc gatgagaatt tcgttgaacg tctgctaaat catccggaag tgccagaact
    43681 gatccgcacc agcccattga cccaacgtga atggcaggtc ctggggctga tttactccgg
    43741 ttacagcaat gaacaaattg cgggcgaact ggaagtggcg gcaaccacca tcaagacgca
    43801 tatccgcaat ctgtaccaaa aactcggcgt cgcccaccgt caggctgccg tgcagcacgc
    43861 gcagaaactg ctgaagatga tgggttatgg ggtgtgagtt tagccggata atgcattgga
    43921 tccggtttac atctttacat cattcaatac tcacccgcgt tacgccatct gtttctatca
    43981 aaccaaaccg caccggcaag aaacgctcca ccaccgcgat attggtcagc agatggcatg
    44041 aaggatgggc gaccttaaat tctcccgcgc ccgccagcgc catcggcagt accagttggt
    44101 cggcgagata ttcccccacc gccgctgggc ttgccaggta gcgtttcacc tctttcacta
    44161 actgcgcggc taccacctcg gcactgacgc gcttttcacc gacgacgaaa aagcgttcag
    44221 tgatattttc actttcgact tccagcgaga cggtattacc cggcccctgg tcgcgcggca
    44281 ggttatgaat attctgttca tgcagggaaa aactccccgc cagtgtagcg atttcacgct
    44341 cagcaacatg gcgcggcaca ccagctaata gaacttctcc acacatctgc acaatgttcc
    44401 cgcgctcgcc aagttgcaag gtgttaaacg atgccacggg cgagacttcc gttgccacca
    44461 caccgcctcc ggcaggataa aaaccgtgac gtaacagcgt ggtttgctga tgaattccta
    44521 ttttcgccag cagcggctcc agcacccggc ggataaaatc cgcaggcggg gccgacgggt
    44581 tatcggtgcc tccgctcact tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca
    44641 gcaccgtttg cagcaccagc gtacagcttc cggcactacc gatagcaaag cggtactcgc
    44701 cgccgcgcac ggtgccaggc cggaagacca gacgctgcga ccccagctcc gcgccttcca
    44761 ccgttgccct acaaatttcc gcagccgctt ttaccgcggt cagatgctgg cgcaacagcc
    44821 ccggtttcgc tcgcccggca cgaatgctgg tgatggtaaa tggctggccg gttatcatcg
    44881 acaggctcag cgccgagcgc aggatctgcc cgccaccttc gccctgtgcg ccatccagcg
    44941 caatcatcct tttcatcatt tatcctttta cgcacaccac ctgacgcagg gtatagataa
    45001 cttccaccag atcgctttgt gccgccatca ccgcatcaat atctttatac gccatcggga
    45061 tttcgtcgat cacttcggca tctttacggc attccacatg cgcggtggca cgaatttgat
    45121 cttccacgct gaacagtttt ttcgctttag tacggctcat tacccgcccg gcaccgtggc
    45181 tgcacgaaca gaacgactct tcatttccca gcccacggac gataaagctt ttcgctccca
    45241 tcgaaccggg aataattcca tattgaccag cacgcgcaga caccgcgcct ttacgcgtca
    45301 cgtagatctc ttcgccaaag tgctgttctt tttgcacata gttgtggtga cagttgatct
    45361 cttccatcgc cagcgtttgc tgttgtctta ccgttttctg cgtaacgctc tgcaacgctg
    45421 ttactacgtt ttccatcatt gcatcgcggt taaggctggc aaaaagctgc gcccaggcca
    45481 ccgctttcag gtaatcatca aagtattccg taccttccat aaagtacgcc agatcacgcg
    45541 acggcaacgt ttcaagctgg tcctgcatct ctttttgcgc cagatcgata aagtaagtcc
    45601 cgatggcgtt accaattccg cgtgaaccgg agtgcagcat aatccacacc tgatccgact
    45661 catcaagaca gatttcaata aaatggttac ccgttcccag cgttcccaga tgtttatagt
    45721 tattggtatt caggaaacgc ggatattttt gcgttaacca ctgataaccg gcttccagct
    45781 cagcccattt agcatcgacg ttaacaggtg gattttccca ggcaccttta tcacgtttac
    45841 aacggccagt ggtacgcccg tgcggcacgg ccgtttcaat cgcctgacgc agctctgcca
    45901 ggttttcagg caggtcttcc gccgttaacg cggtacgcag cgcgttcatt ccacagccaa
    45961 tatccacgcc caccgccgcc ggaataatcg cccctttggt cgggatcacg ctaccaatgg
    46021 tggtaccttt acccaggtgt acatcaggca ttaccgcaat atgtttgaaa ataaacggca
    46081 tcttcgccgt attaataagt tgctgacgcg catcggcttc taccggcacg cctttggtcc
    46141 acatttttac cggggcattt tcagtgggca gtaattcgta attcattttg tttttctctt
    46201 ttcgttgttt gctgtcgtga taattgcaac cgtcgtgcca gaaaattaaa taagcagatt
    46261 aattttttaa ttcattgttt ttaaataggt taaatcttta cgtccgtaaa cggagatttt
    46321 ccacaaaacc aattcaccga taatgaaata tcgtctttta taaggatatc taagatgcgt
    46381 aaaacagtgg cttttggctt tgtcggtacc gtgctggatt atgccggacg cggcagtcag
    46441 cgctggtcga aatggcgtcc gacgctttgt ttatgccagc aagaatcgtt agtcatcgat
    46501 cggctggaat tgttgcacga cacccgctcg cgctcgctat tcgaaacgct taaacgcgat
    46561 atcgccagcg tttcgccaga aacagaagtc gtgggcgttg agattgaact gcataacccg
    46621 tgggatttcg aagaggttta cgcctgcctg catgatttcg cccgtggtta cgagtttcag
    46681 ccagaaaaag aagactattt aattcacatc accaccggca cccacgtcgc gcagatttgc
    46741 tggtttctgc tggcggaagc gcgttacctg cccgcccggc tgatacaatc ttcacctccg
    46801 cgcaaaaaag aacagccgcg cggcgcaggt gaagtgacga ttatcgatct cgatttaagc
    46861 cgttataacg ccatcgccag ccgctttgcc gaggaacgcc agcaaacgct tgattttctt
    46921 aagtccggca ttgccacgcg taacccccac ttcaaccgca tgattgagca gatcgaaaaa
    46981 gtggcgatca aatcccgcgc gccgattctg cttaacggtc caaccggcgc gggcaagtca
    47041 tttctggcgc gacgcatctt cgagttaaaa caggcgcggc atcagtttag cggcgcgttt
    47101 gtggaagtga actgcgccac cctgcgcggc gataccgcca tgtcgacgct gtttggtcat
    47161 gtaaaaggcg cgtttaccgg ggcgcgggaa tctcgtgaag gtttattacg cagcgccaac
    47221 ggcggaatgt tgtttcttga tgagattggc gaactgggcg cagacgaaca agcaatgctg
    47281 ctaaaagcca ttgaagagaa aaccttttac ccgtttggca gcgatcgcca ggtgagcagt
    47341 gattttcagc ttatcgccgg aacggtgcgc gatttgcgcc agctggttgc cgaaggcaaa
    47401 tttcgcgaag atctgtacgc gcggatcaat ctctggacct tcaccctgcc gggtctacgc
    47461 cagcgccagg aagatattga accgaacctt gattatgaag tggagcgcca cgccacactc
    47521 actggcgaca gcgtgcgttt taacaccgaa gcgcggcgcg cctggttggc ttttgcgacc
    47581 tctccccagg caacatggcg cggtaacttt cgcgaacttt ctgccagcgt cacgcggatg
    47641 gccacctttg ccactagcgg acgcatcact ctggacgtgg ttgaagatga gataaaccgt
    47701 ctgcgctata actggcagga gagccgcccc tccgcgctta cggcgttgct gggcgcagag
    47761 gcagaaaaca tcgatctctt cgaccgtatg caactggaac acgttatcgc tatctgccgc
    47821 caggcaaagt cgctttctgc agccggacga cagctttttg atgtttcgcg ccagggcaaa
    47881 gccagcgtca atgacgcgga tcggctacgc aaatacctgg cgcgttttgg tctgacgtgg
    47941 gaagccgtgc aggatcagca cagctccagt tgaatatgat ggtccgtcag cacctgcatt
    48001 acgcttactg gcggcggggc gtcggtgtag acggcatcta ccatgctgat gctgcccata
    48061 ttgaccatcg cgttacggcc aaatttcgag tgatcgacaa ccagcataac gtggcgcgag
    48121 ttctcaataa tggcgcgctt ggtgcgaact tcgtggtaat cgaactccag cagcgagccg
    48181 tcgctatcga tgccgcttat ccccagaatg ccgaaatcaa ggcggaactg ggagataaaa
    48241 tcgagcgtcg cttcgccaat gatcccgcca tcgcggctgc gtaattcgcc accggcgaga
    48301 ataatgcgga aatcttcttt taccatcaac gtgttagcaa cattgagatt gttggtgaca
    48361 atacgcaaat tgctgtgatt aagcagcgcg tgcgccaccg cttccggcgt ggtgccgata
    48421 tcgataaaca gtgtcgagcc attggggatt tgctccgcca ctttgcgggc aatcctctct
    48481 ttttcttcgg tctgggtggc tttacgatcg tgccacggcg tgttaaccga actggaaggc
    48541 agcgccgcac cgccatggtg acgcaggatc aggttttgct ccgccagctc attgaggtcg
    48601 cggcgaatag tctgcgggct gacggagaaa tgctctacca gctcttcagt actgacataa
    48661 ccctgctgtt taaccagttc gataataccg ttgtgacgtt gtgtttgttt catttataaa
    48721 tccctggaat tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca tcgctaaacc
    48781 cacggctaac ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca aatcaaacca
    48841 tccggcgaca atccagatca gcgcaaagat aattaaccca cgttgcaggt aaatgccact
    48901 ttgcggatcg cgttcgccac gtagccagac gtagcccatc agcgcataca ccacgccaga
    48961 aagcccgcca aaccacggcc cgctgaattt ttgctgcaca tagccgctta acagggcgct
    49021 aatgagagta ataacaatta gcttaccgct accgaggcgt ttttccaccg caccgccgag
    49081 ataccaccac cagagcaggt taaagaggat atgcatcagc gagaagtgca ttaacgcgtg
    49141 ggtgaagtaa cgccagaact caaatttcag tgttggatcg aatggccagg ccagccataa
    49201 catcacttcc tgatcgccga gaatttgcat ggcgataaac accaccacgc aggcgatcat
    49261 caccacccag gttaccggac ctgcgcgttc acgcaaggca gcaaagaaag gataacggcg
    49321 ataatgcaga ccactgccgg tatggcctgc ctgccagctg gccgccagat aacgcggatc
    49381 tgccgggttt tcgagaaaac gcgccagctc cgcccgtacg cgctcggcct gggactcatc
    49441 cgccagccag acatcgcttt ggttatgttg ttgaatcgtg aggataacac cctgcgtcgc
    49501 catgtaatca acaaacgcct gcgccacgcg ggggttagca aaagaggtaa tcatcaacat
    49561 cgttgctgtc gcttattcca cacaaaaggg gacagtataa agcgttacgc gccgtacgcc
    49621 acctctgcgg gaaactgacg ctgccaggct tcaaaaccgc cgtcaatgct atagaccaca
    49681 tcgtagccct gttgcagcag atactgcgcc gcgcctttgc tgctattgcc gtgataacac
    49741 atcaccatca ccggagtgtc aaagtcgtta tcacgcataa acgcgcccag cgtgtcgttg
    49801 gttaaatgga aagcctgcac cgcatgcccc attgcgaaac tctgtggatc gcgaatatcg
    49861 accagcaccg cctctttttc ctgcaacttc tggtgcgcga cggcaacgtt aatacattcg
    49921 aactgatcca tgcgtctctc tttctttaca aacaagtggg caaatttacc gaacagttta
    49981 cgtcgaagcg gcagataaac gccataatgt tatacatatc actctaaaat gttttttcaa
    50041 tgttacctaa agcgcgattc tttgctaata tgttcgataa cgaacattta tgagctttaa
    50101 cgaaagtgaa tgagggcagc atggaaacca aagatctgat tgtgataggg ggcggcatca
    50161 atggtgctgg tatcgcggca gacgccgctg gacgcggttt atccgtgctg atgctggagg
    50221 cgcaggatct cgcttgcgcg acctcttccg ccagttcaaa actcattcac ggtggcctgc
    50281 gctaccttga gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa cgtgaagtgc
    50341 tgctgaaaat ggctccgcat atcgccttcc cgatgcgttt tcgcctgcca catcgtccgc
    50401 atctgcgccc ggcgtggatg attcgcattg gtctgtttat gtacgatcat ctgggtaaac
    50461 gcaccagcct gccgggatca actggtttgc gttttggcgc aaattcagtg ttaaaaccgg
    50521 aaattaagcg cggattcgaa tattctgact gttgggtaga cgacgcccgt ctggtactcg
    50581 ccaacgccca gatggtggtg cgtaaaggcg gcgaagtgct tactcggact cgcgccacct
    50641 ctgctcgccg cgaaaacggc ctgtggattg tggaagcgga agatatcgat accggcaaaa
    50701 aatatagctg gcaagcgcgc ggcctggtta acgccaccgg cccgtgggtg aaacagttct
    50761 tcgacgaggg gatgcatctg ccttcgcctt atggcattcg cctgatcaaa ggcagccata
    50821 ttgtggtgcc gcgtgtgcat acccagaagc aggcctacat tctgcaaaac gaagataaac
    50881 gtattgtgtt cgtgatcccg tggatggacg aattttccat catcggcacc accgacgtag
    50941 agtataaagg cgatccgaaa gcggtgaaga ttgaagagag tgaaatcaac tatctgctga
    51001 aagtgtataa cgcgcacttt aaaaaacagc ttagccgtga cgatatcgtc tggacctact
    51061 ccggtgtgcg tccgctgtgt gatgatgagt ccgactcgcc gcaggctatt acccgtgatt
    51121 acacccttga tatccatgat gaaaacggca aagcaccgct gctgtcggta ttcggcggta
    51181 agctgaccac ctaccgaaaa ctggcagaac atgcgctgga aaaactgacg ccgtattatc
    51241 agggtattgg cccggcgtgg acgaaagaga gcgtgctgcc gggtggtgcc attgaaggcg
    51301 accgcgacga ttacgcagct cgtctgcgcc gtcgctatcc attcctgact gaatcgctgg
    51361 cgcgtcatta cgctcgcact tacggcagca acagcgagct gctgctcggc aatgcgggaa
    51421 cggtaagcga tctcggggaa gatttcggtc atgagttcta cgaagcggag ctgaaatacc
    51481 tggtggatca cgaatgggtc cgccgcgccg acgacgccct gtggcgtcgc acaaaacaag
    51541 gcatgtggct aaatgcggat caacaatctc gtgtgagtca gtggctggtg gagtatacgc
    51601 agcagaagtt gtcgctggcg tcgtaaggat gatgcccggt tgtgaagtgc aaccgggcta
    51661 tttggattcc ttagactatt cattgtaaat aattgaataa cggcccctga gctaaaatcg
    51721 ccaaacttaa gcacgttacc caatcgcccc acttcttcct ctggtttcta ttttttctgc
    51781 accaatggca ataatatccc aatgcaaaac caagctgtgc actaatcggg ccgacaaaat
    51841 cagtccaagg ctgcggccaa aaagggaaaa attcagggga agccagacaa acgtcagaaa
    51901 gaaatttcgg atattttcca gcaacgctga tttctctgga gaaatataaa gaatgaaatt
    51961 cgagagaatt aaccagaaat agaccggcga aaagaccagc cagcaatgca gtctgttttt
    52021 taattcagga ggcgttctgc gggagaattg ccaaccgatg ccagccatta cactgaaaaa
    52081 aagtgcatat tcaaccatta actcaagttg tagttcctga agagaaatac gcacaaccag
    52141 gagcggaaag gagaatatta tttctgccag aaaagttaac gtgaatataa gtacggctaa
    52201 gacaataacc gtcggccata ccaataatgg cacgataaca aaaccgagaa ttacaggggg
    52261 aatataaagc cggaatttaa tcatttttat tttcctggtg attcggatga tgcgtcatac
    52321 atcgcgcata aacactcatc aattatccag tcgtctaccc agacctgatg ctgtgtgccc
    52381 tgttctggct gctttggtgg gttctcaatc gcctgccgcg cgcgttcttt caagatggat
    52441 tgattgttga aggcccaggc attgatttgg gtggtaatga cgttatcgat atcgtcctgg
    52501 ttatctctga tataatcctc acaatatctg ttcaataaac aggcaagaga aacggtcgac
    52561 caaaagccgg gaactgaatt aatggcatcc agcaatggcg caagagcgat atcggcacgg
    52621 aaaaacattt ccagcatata ctgtaattcg acagcttcat cgccggatac ccaatcagtc
    52681 atatactggc aggcaaatcg ttgcaaaatt ttccattcat cctcacgcca attttcttta
    52741 ctggcaaggt ttatacgccg tagagaaaat acttcgtgaa tattgtcaaa ctgcccctgt
    52801 gcaacaaatt ccagtaaacg cggcagaaaa tagcggacct catcgctatt ttgcggatca
    52861 ggccctgggc tactattcca tgcattgatt aaggaatacg agatcgctcg taacgatgta
    52921 ctccgtaata ccttttgttc ctgctgactc aggcaatatt cacaacaaac cacaaatttc
    52981 tgcggcaccg gataacgacg aaaaatatga taaatctgct caattaatgc ctgcatggcg
    53041 gggctgggtt gcataattac cccctcgccc gctgatcgcg tgtaaacggt tgataaacaa
    53101 aacgagcatt cgccacgttc aggcgaataa tggcactacg agagagggaa aagctggggc
    53161 gaatgcaact attgcagtct cgacagcttt cttcagcgca attttccact gtgtaataac
    53221 gagcgtagag tttcagttcc gcttgcttat cacgaaatcc gcgtaaactg ttatcgttat
    53281 gaaatttcca ggcatcttta aagacctgaa aaatgtagat atcctcaatc caggactcac
    53341 attttttaag cgggcgcttt gctatgatca cccgctcatc cgggtttagc atcgccgtaa
    53401 attgaacggt gaggtcattt gtcgtgttca gatcacattg aacatctggt acatcactga
    53461 tataggggtc aaatccaaag tcagcaccac aggcacaaag acggctaatg gtggcggtat
    53521 cgagggaaaa tcccggccat acattgctgg acgttaagcc aatatttaat gtgacattat
    53581 atcgaagcaa tccctcaaga ccgtcagcac cgacgaactt ttccccttca tccagggcct
    53641 ggttaatgag ttgctgaaca atgccttcat cccagtatgc cgatttgcag ttggcaatcg
    53701 taatatccac gcctgtgaat ggatcagtac cgacaaggct tgcaaataat gtattaacta
    53761 attccatttt tattatccgc cttaatgcaa cgttttatac atcggcacat aacctacatc
    53821 ttccaccagg ctctgcccct gcggcgttaa aaaccactcc accaattttt gtgtttctga
    53881 cgtcatattt tcccgcgtta ccataaaggc atcgacgata tagggatatt tgccgttgcg
    53941 aatgttttcg gcagtcggtg caataccgtt aatcgctagc agttttatat ttttatcggc
    54001 gttcatttgt gttgcgtaat aacggaaggt gtaacctatt gcgttgtttg tattacggta
    54061 ttcggcaacg actttaatca ttccctccat catacttgcc acttctgttt cctgcggcga
    54121 gatcatgcgg acatttttca tgacctggga ttgcatcacc gtctggctac cagagtcttc
    54181 cgggcgttgc caggtctgga tctcctgatc gttaccgcca acagtacgcc agttggtaat
    54241 tgcaccactg aagatgtcac gcacctgttg ttcagtcagg gaattaaccg ggttatctac
    54301 attgacgatg aaaacaaacg cttcacgggc aaaaggcgtg taaatcaaag tgacgcccga
    54361 ttcctccgcg cgttttttct gcccaccgga aggttgagcc acaaagataa tatcggcatt
    54421 acctttaaca atcttgttat atgcttcggg agtacgagag ttcgccagat attcccattc
    54481 gtgaaaatct tccggcaaaa cgcttagtgc ataaaaggca gaggcataaa tggggtacgc
    54541 cgccgttgcg ccatcaagtc gcggccagtt ttgcgtgaac tgaatttgcg gtttaccacg
    54601 cagtggtgtc agttgattat tgagtttatc gggtcgccag gcccaggtat caatattttc
    54661 acgcacaccg acgccgggat taagctgttt atataaataa gcctggtaac cggcgacaaa
    54721 accaagcatg agaaggataa acaagagaga attacggcaa cgtaacccgg acgtgcccgt
    54781 taaaggatat ctggtcggcc agccgtatcc cagcgaaaaa ctgatatgcg cgccaacagg
    54841 tataatgact aaccaccagt actcaccacc gactaatgag gctaacaaaa cgcccagcca
    54901 ggggagcata agatcagcca accaacccgc tcccatctgg cgaaattgat aatctgccag
    54961 atgcagcgcg attatccaga cccccagggt taataatgcg ggtactaaaa aaagacattg
    55021 tcgccagacg gctatttctc ttttttctgg tgcactatcg tccgcccaca ctcgccccga
    55081 gaaaaatagc attaatgcgc ccagcgccag cataaaaatg ttgagcagaa ggtaaattgt
    55141 tggcgtgata aagatattac aaacttcaat aataccggca agtccgacac ccagcatggc
    55201 aataaccgcc gccaaaaact gcgccagaat ggggatgccg aaaaaagtca ttaccagcga
    55261 ggtcaaaatc cattttctgt tttgcattat tctttccatt ctttctgaat ggcgaaatta
    55321 tactccccga gtccccttgc cccttctgga cacttttccg aaatgatggc gaaaaaaacg
    55381 ggaccctttg gccccgttct atttattagt gaaattacaa tctcaccgga tcaatatgcc
    55441 agatatgatc ggcgtactct ttgatagtac ggtcagaaga gaagtagccc atattggcaa
    55501 tgttcagcat cgcttttgcg gtccactctt cctgaagctc gtagagttcg tcgactttat
    55561 cctgacaatc gacatagctg cgataatccg ccagaacctg atagtgatcg ccgaagttga
    55621 tcagcgaatc aaccagatcg cgatagcgac ccggatcttc cggactgaat acaccgctgc
    55681 cgatttgcgt cagcacctga tgcagctcct catctttctc gtagtattca cgcggtttgt
    55741 agccctgacg acgcagttct tccacttctt ccgcagtgtt accaaagata aagatattgt
    55801 cagcaccgac atgatccagc atctcgacat tcgcaccgtc caacgtaccg atagtaagcg
    55861 caccgttaag cgcaaacttc atgttactgg tgccggaagc ttccgtcccc gccagcgaaa
    55921 tctgttcaga cagatcggcc gccggaatga tcaactgcgc caggctaacg ctgtagttcg
    55981 ggatgaacac gactttcagc ttgtcgccaa tctgcgaatc gttgttgatc actttcgcta
    56041 cgtcattgat caaatgaata atgtgcttcg ccatgtaata ggccgaagcc gccttaccgc
    56101 caaaaatatt cacgcgcggt acccacttcg catccgggtc ggccttgatg cggttatagc
    56161 gggtaatcac atgcaacaca ttcatcaatt gacgtttgta ttcgtgaata cgtttgattt
    56221 gtacatcgaa caacgccttt ggattcacca ccacattcag ctgctgggcg atatactctg
    56281 ccagacgctt tttgttctcc agcttcgcct gatgcacagc gtgattaacc attgggaaat
    56341 cacagtgttg ttgcagctca ttaagcaggc taaggtcggt gcgccagtta cggcccaggt
    56401 gttcgtccag cacggctgaa agcgatgggt tcgctaccgc tagccagcga cgcggcgtca
    56461 caccgttggt gacgttggtg aaacgacccg ggaagatttt cgcaaagtcg gcaaacaacg
    56521 attgcaccat cagattagag tgcagttccg atacaccgtt aactttgtgg ctcacaacaa
    56581 ccgccagcca ggccatacgc acacgacgac cgttggattc atcaatgatc gacgcccgtc
    56641 ccagcagatc ggtatcgttc ggatactgtt cctgcaaggt tttcaggaaa tagtcgttga
    56701 tttcaaagat gatctgcagg tgacgtggca gaattttacc cagcatatca accggccagg
    56761 tttccagcgc ctcgctcatc agcgtgtggt tagtgtagga gaagacctga caacacacct
    56821 caaacgcgtc gtcccagcta aattggtgct catcgatcag cagacgcatc atctcaggaa
    56881 tcgacagtac cggatgggta tcattgagat gaatcgcgat tttatccgcc aggttatcgt
    56941 aggttttatg caactgataa tggcggctta aaatgtcctg aatggtcgag gaaaccagga
    57001 agtattcctg acgcaggcgc agctcacgcc cggagtaggt ggagtcatcc ggatacagta
    57061 cgcgagatac gttctcggag tggtttttat cttccactgc cgcgaagtag tcaccctggt
    57121 tgaatttacc gaggttaatt tcgctactgg cttgcgcact ccacaaacgc aacgtgttgg
    57181 tcgcgtcggt gtcgtaacca gggattatct gatcgtaagc gactcccaga atctcttcgg
    57241 tttcaatcca gcgcgttttt ttaccttcct gctgaatgcg accaccaaaa cggactttat
    57301 agcgcgtgtt gtggcgtttg aattcccacg ggttaccgta ttccagccag tagtctggcg
    57361 actctttctg gctaccgtta acgatgttct gcttgaacat accgtagtca tagcggatgc
    57421 cgtaaccgcg ccccggcaac cctaacgtcg ccagagaatc aaggaagcaa gccgccagac
    57481 gtcccaggcc accgttaccg aggcctgggt cattttcttc atcaatcagc tcttcgagat
    57541 ttaaccccat cgcttccagt gcgccctgta catcttcgta aattcctagc gacaacatgg
    57601 cgttggagag cgtacggcca atcaaaaact ccatcgacag gtagtaaacc tgacgagttt
    57661 cttgcgacaa ctgggcacgg tttgaacgta accagcgctc cacgagacga tcgcgcacag
    57721 caaataacgt tgcgttcagc cattcatgtt tattggcgac gaccgggtcc tttccaatcg
    57781 taaacatcag cttgtaagcg atagagtgct taagagcttc tacgctaagc gtgggcgatg
    57841 aatatgtaaa cggagcattc atataggcgt ttcctgaaaa ctatttcaag cgatagtaaa
    57901 gctcacggta cgacttcgcc gcgacctgcc agctaaaatc cattgccata gcctgacgtt
    57961 gcacaaaccg ccacagtgaa ggacgggacc acagtacaaa agcacgccga atagcccgta
    58021 acagcgacca ggcattacta tcttcaaaga caaacccact ggcgacgcca tctgcaaggt
    58081 tctcaagaga acagtcagaa accgtatcag caagcccacc ggtgcgccgc actaacggca
    58141 gcgtaccgta cttcaatcca taaagttgcg ttaagccgca cggttcaaaa cggctgggca
    58201 ccagaatgac gtccgcgccg cccataatgc gatgcgaaaa tgcttcgtga tagccaatct
    58261 gaacgcccac ctgaccgggg tattccgctg ccgccgcaag gaaaccttcc tgcagcaccg
    58321 gatcgcccgc gccgagtagc gccagctgcc cgccctgctc cagaagaccc ggtaaggctt
    58381 ccagcaccag gtcgagacct ttctggctgg tcagacggct caccactgca aaaagcggca
    58441 ctttatcgtc aaccttaagc cccattgcga tttgtaactg gcgcttattt tccgctttat
    58501 cttccaacgt atcgcgggtg taacgcgagg ccaacagtaa gtccgtctct ggactccaga
    58561 ttttctcgtc cacgccgttc agtacgccgg aaagacgccc ttcacggtga cgctgttgca
    58621 acagaccttc cataccgtag gcaaactgcg gttcggtgat ctcgcgagcg taggttggac
    58681 tgaccgccgt aatgtgatcg gcatagtaca gaccggcctt caggaaagag atttgtccgt
    58741 tgaattccag cccatgaata ttaaagaatg accatggcaa ttggatgtca ttcatatgat
    58801 gtgcataaaa catgccttga taggccaggt tgtgcacagt aaacaccgac ttcgccggac
    58861 gcccgcgcgc cgccagatac gcaggcgcaa ggcctgcatg ccagtcgtgc gcatgcacca
    58921 catcaggacg ccagaatggg tcaagcccgc tggccatttc tgccccaacc caccccagca
    58981 gcgcaaaacg caatacgttg tcggtatagg caaataagtt ggtatcgtga tacgggcttc
    59041 ccggacgatc atagagatgc ggcgcgtcaa tcaggtaaat gccaaccccg ttgtaatgac
    59101 cgaacaacag cgtgatatgt ccggcgaagg tatcacgacg ggatactacc tgcgcatcgg
    59161 tcacgccacg gcgaatatcg ggaaatgcag gcaacagtac gcgagcgtca acgccgtctg
    59221 cgatttgtgc tgcgggtaat gccccaataa catcagccag accgccggtt ttaagcagcg
    59281 ggaacatctc tgaacataca tgtaaaacct gcattatcgc tcctgtttat gccctaactt
    59341 ccgtagcatt tcgcgcgtta ccagcacgat gccttcttct gaacgataga aacgacgtgc
    59401 atcttcctct gcgttttcac caatcaccat gccttccgga ataacacaag cacgatcgat
    59461 gacgcagcgg cgcagacggc acgagcgacc tacccatact tccggtaaca atacggcgga
    59521 atcaatgttg cagaatgaat tcacgcgaac gcgcgagaac agaacggact gcaccaccac
    59581 cgaaccggag atcacacagc cgccggatac cagtgagtta agggtcatcc cgtggctacc
    59641 ggagcgatcc tgcacgaatt tcgctggcgg taatgattca ttgtaggtgc gaattggcca
    59701 attgcgatcg tacatatcca gttccggcac cacagaggcc agatcgaggt tcgctttcca
    59761 gtaagcttcc agcgtaccca catcgcgcca gtacggctcg gcatccgggt cggattgtac
    59821 gcaagagagc gggaacgggt gcgcataggc cagaccggct tcggtgatct tgggaatcaa
    59881 atctttgcca aagtcgtggc tggagttctc atcacgatcg tcttcttcca gcagttcata
    59941 cagatagtcg gcgtcaaaga cgtagatacc catactcgcc agagatttgc tcggatcgtt
    60001 cggcattgac ggtgggttag caggtttttc aacgaattcg ataattttat cgttctcatc
    60061 aaccgccata acgccaaatg cggaggcttc ttcaatcggt actggcatac aagcaacggt
    60121 gcaacgtgcg cctttttcga cgtgatcgat aagcatacgc gagtagtctt gcttgtagat
    60181 atggtcgccc gccaggatca ccacgtattc cgctttataa cggcggataa tgtcgaggtt
    60241 ttgggtgacc gcatctgcgg tgccgcgata ccagttttcc cctttcattc tctgctgtgc
    60301 tggcagcaga tcgacaaact cgttcatttc ttcattgaag aatgaccagc cgcgctgaat
    60361 gtgctgcacc agagtgtggg actggtactg ggtgatcacg cccatacgac ggatcccgga
    60421 gttgatgcag ttagacagcg caaagtcgat aatgcggaac ttaccgccga agtgtacggc
    60481 cggttttgct cgcttattgg ttaaatcctt caggcgggta ccacgtcctc ccgccagtat
    60541 cagggcaaca gatttcaatg gcagctggcg cgccaacatt aagtgatcgt tcttctctaa
    60601 actaaccatg actaactcct tttttatcat ctctggaaca cacacaatcc gtgtgcaggt
    60661 ccctgccaga cagccgtaat cactgggtta tcctctccag cgaatggggg aatggcgtgc
    60721 cactccccag caggtaaaac aatctctgtt acctcaagcg tggcgttaat tgcgatcaaa
    60781 aagcgatccg agagcagaat ttgcagctgt ttcggcccgt tttgccactc atccgtgctt
    60841 aaaggttgag catatcgatt tagccaacgg acattgccgt cgccttcttc ccaccagcga
    60901 ttctccacca aagcgggaat gcgcttgcgc agatggatta acgcggcggt aaatgcggtt
    60961 aaaccactgc ttgcctgcga ccagtccaac caggttaatt ggttatcctg acagtaggca
    61021 ttgttattgc catgctggct gtgaccatgt tcgtcaccgg ccagtaacat cggcgtaccc
    61081 tgggagagca acaacgttgt taacagggcg tgaatgctgt cgcgccgccg ttcaaccagg
    61141 tcaagagaac cgcctaaccc ttctttacca tgattgttac tgtaattgtt gttggtcccg
    61201 tcgcgatttt cttctccgtt tgcttcattg tgtttatggt tgaaacaaac gcagtcgcga
    61261 agcgtaaaac cgtcatgcgc ggtgacgaga ttaatcgcgg cactcggcag acgaccatta
    61321 cgtttaaaaa catcgctgga ggcagcaaaa cgcccggcaa acgcccccag aggcaaatca
    61381 taatgtagcc agaaacgacg ggcagcatcg cggaaatgat cgttccactc ggcaaacagc
    61441 ggcgggaaat ttcccacctg ataaccacca ggagcgatat cccacggttc agcaattaac
    61501 ttcacctgcg agagcaccgg gcagttctgg atagcggtaa acaacggcgc atcctgacgg
    61561 aactctggcg tacggcccat gactgccgcc agatcaaagc ggaaaccatc gacgtggcag
    61621 gtttctaccc aataacgcag gcaggcgctg gcataatcca ccaccgccgg atgactcaaa
    61681 ttgagcgtgt tgccgcaacc ggtccagttg tgataatcgc cgtcttctct tatccaataa
    61741 tagctacggt tatcgatccc acgcagcgag aataacgggc cgtcgaggtc cagttccgca
    61801 ctatggttga gcacgatatc aagaatgact tcgatacccg ctttatgcag tgctttgatt
    61861 gcatcgcgaa actcatccag cgccgtttct ggcgagcagg catacgccgg atgcagcgca
    61921 aacatcgcca ccgggttgta accccagtag ttacttagcc ccatgcgttg cagacgtggt
    61981 tcactggcaa actgcgccac tggcagcagt tccagcgcgg taatgcccaa ttgtttcaaa
    62041 tagttgatca tcaccggatg cccgagggct ttataagtgc cacggatctc gaccgggatc
    62101 tccgggtgca agtacgttaa tcctttgaca tgggcttcat aaatgatggt gctgccccac
    62161 ggcgtgcgcg gcggggcatc atcttcccag tcatagtgat caaccaccac tacgcatttc
    62221 ggcgcaatgg cggcgttgtc gcgatagtca ggttcattat gaccggcgtg cagcagcggg
    62281 ttatctttaa actccccgtc aatttgccgc gcgcaaggat caatcaacaa cttcgccggg
    62341 ttaaagcgat gcccctcggc gggttgccag gggccatgaa cgcgataacc ataacgcaaa
    62401 cccgggcgcg catccggcag ataaccgtgc caaatgtcgc cactgtgccc tggcaagtca
    62461 tagcgatgtt cctggccatt ggcgtcaaag acacacagtt ctacccgctc ggcatgagcg
    62521 gagaaaagtg tgaagttgac gccctgaccg tcgtaatgcg cgccgagggg agcgggtttg
    62581 ccaatggcga gtcgtgtcat tctgcctccc gaaccagcca gatagtggcc agcggtggta
    62641 gtgtcaggct tagtgaatgc tgacgaccgt ggctggcaat ctcatcgctg tgtaccgtgc
    62701 cgccattgcc tgcattactg ccgtgatagt gcatggaatc ggtattgagg atttcacgcc
    62761 atttgcctgg ctgattaatg ccgaagcgat aatcatgacg cggcaccggc gtaaagttac
    62821 tggcaacgat aatttcgtta ccctctttat cgcgacgcac aaagatcagc accgagcgtt
    62881 ctttgtcatc caccaccagc cattcaaagc cgtacgggtc aaaatccagt tcatgcattg
    62941 ctttatggtg gcggtaggtg aggttcagat cgcgcaccag acgctggaca ccgtggtgcc
    63001 agttatcgcc gccttccaac agatgccagt cgaggctggc gtcatggttc cactcacggc
    63061 cttgggcaaa ttcgttcccc atgaacagca gtttcttgcc cgggaatgcc cacatccagc
    63121 catagtaggc gcgcaggtta gcgaatttct gccatgcgtc gcccggcatg cggtcgagaa
    63181 tcgatttttt accgtggacc acttcatcat gcgacaacgg caggacgaag ttttcagtgt
    63241 agttgtagag aatcccgaag gtgagtttat cgtgatgata ctgacgataa accgggtcga
    63301 gcttcatgta gtccagggtg tcatgcatcc agccgaggtt ccacttgtac cagaagccca
    63361 gaccgcccat atcctgcgga cgagaaacgc cagggaaatc ggtagactcc tcagccattg
    63421 tcaccgcacc ggaaacctgc tcaccaagaa tacggttggt attacgcaag aattcaatcg
    63481 cttcaagatt ctcgcgcccg ccaaattcgt tcgggatcca ctccccctct ttacggctgt
    63541 agtcgcgata aatcattgac gccaccgcat cgacgcgcag cgcatcaata ccaaaacgtt
    63601 caatccagta aagcgcgtta ccgacgagga agttactgac ttcacggcga ccatagttgt
    63661 agatcagcgt attccagtcc tgatgatagc cttcgcgcgg atcgctgtgt tcatacaagt
    63721 tcgtgccatc aaattcggca agcgcaaagt catcggtcgg gaagtggcct ggcacccagt
    63781 cgagaatcac gttcagaccc gctgcgtgtg cggcatcaat gaaataacgg aagtcgtcgc
    63841 gagtaccaaa acggcgggtt ggtgcataca ggccggttgg ctgataaccc caactgccat
    63901 cgaagggatg ctcgttaatg ggcagtagtt cgaggtgggt aaagcccatc catttagcat
    63961 aaggcaccag ttgatcggcc agctcgcggt agctcaacca gaaattgtta tcggtgtggc
    64021 gacgccagga acccaggtga acttcataaa tagagattgg cgcatcaaac tgattcgctt
    64081 ttttgcgctc ttcagtctgt acaacctttt ccggcagccc gcaaataaga gacgcggttt
    64141 ccgggcgcat ttgcgcttcg aaggcataag ggtcggactt cagacgcaag ttgccattgg
    64201 catcaatcat ctcgtattta tagagctgac cgttatgcgc cccagggata aacagttccc
    64261 aaatgccgct ctctttacgc agacgcatcg ggtgacggcg accgtcccag tagttgaatt
    64321 gcccaaccac cgagacccga cgggcgtttg gagcccagac agagaaacgc gtacctgtga
    64381 cgccatccat agtatctgca tgcgcgccta aggtttcata cgggcgcagg tgagtacctt
    64441 cagataatag ccaggcatcc atttcctgga ttagcgggcc aaaacggtaa ggatcatcaa
    64501 tcaggttttg ctgaccatgc cagacaacag ccaactgata gcggaaaaaa ttcttacgtc
    64561 gcggaatgac accgctaaag aatccccgtg agtcgagaca ctccagtttt gcgagtttgc
    64621 gcccggtttt cggttcaatc acccacacat cggtagcgtc gggtaaaagg gcacggactt
    64681 ccagtcccgc ggtggtttta tgcattccca gtacggaaaa aggatccgca aaatggcctg
    64741 caattagcgc gttaatcacg tctctatcga tacgatcgga catgcttgtc ttcctgtttt
    64801 attgtgtcac cccatccaat tggatttttg acttctggtt gtgacatttt tttgacctga
    64861 acggcgcagc actctgtgca tcctctctgc gtcgtcctca cttcaggtaa ggctgtgaat
    64921 actcatgtat tcagccaccc ttaaagaata gccaatgctc tatttaactc ccggtaaatc
    64981 atgaaacatc tgcgcttact cctgtattac gcactaacag gggcggcatc gcgccccaga
    65041 tttaatgaat aaagattacg ccagttgacg aagcatccga cgcagcggct ccgcggcccc
    65101 ccacagcagc tggtcgccca cggtaaaggc tgacaggaac tctggtccca tattcagctt
    65161 acgcaggcgg cctaccggcg tggtcagcgt gccggtaacg gcagctgggg ttagctcacg
    65221 catagtgatt tcccgatcgt tcggaacgac tttcgcccac ggattgtgcg cagccagcag
    65281 ttcttccacg gtcggaatag acacatcttt tttcaattta atagtgaatg cctggctgtg
    65341 gcagcgcaat gccccgacac gcacacataa accatctacc ggaattacgg aagatgtgtt
    65401 gaggatcttg ttggtttccg cctgcccttt ccactcttcg cggctctgac cgttatcgag
    65461 ctgtttgtcg atccacggaa tcaggctacc cgccagcggc acgccaaagt tatccaccgg
    65521 cagctcaccg ctacgggtta aggttgtgac tttgcgttcg atatcgagaa tagcagagga
    65581 cggggtcgcg agttcatctg ccacatggcc atacagatgg cccatctggg ttaataactc
    65641 acgcatatgt cgcgcaccac cgccggaagc ggcctggtag gttgcaacgg acacccaatc
    65701 aacaagatca ttggcgaata aaccacccaa cgacatcaac atcaggctta cggtacagtt
    65761 accgccaaca aaagtcctga tgccattatt taatccgtcg gtaatgacgt cctgattgac
    65821 ggggtcaaga atgatgatgg cgtcatcttt catgcgcaga gacgatgctg cgtcaatcca
    65881 gtaaccttgc catccgcttt cacgaagctt tggatagatt tcgttggtat aatcgccgcc
    65941 ctgacaggtc acaatgatat cgagggcctt tagcgcctcc agatcaaagg catcctgaag
    66001 tgtgccagtg gttccgccaa aagacggcgc agcctggcca agctgagaag tagaaaagaa
    66061 gacagggcga atggcgtcga agtcgcgctc ttcaaccatg cgttgcatga gaacggagcc
    66121 gaccataccg cgccagccga taaaaccaac atttttcata agcgtttttt cctgcaaaga
    66181 tgtgtgctgt ataaatgtgc cggtctcctc ttggcacatc tttcaccata caaaaagcag
    66241 ccaaagtcgc aagtgaaatt aatcaatgat agcgaagcca tcagtaatgc gacttatcct
    66301 gctttgttag cacgcagaaa gtccgcggca attatcaggg aatttgagtt atgaatgaaa
    66361 tcatttctgc agcagtttta ttgatcctga ttatggatcc gctcggaaac ctacctattt
    66421 tcatgtccgt actgaaacat actgaaccga aaagacggcg ggcaatcatg gtgcgagagt
    66481 tgcttattgc tctcctggtg atgctggtgt tcctgtttgc gggtgagaaa attctggcat
    66541 ttctcagcct gcgagcagaa accgtctcca tttctggcgg catcattctg tttctaatcg
    66601 ccattaagat gattttcccc agcgcttcag gaaatagcag cgggcttccg gcaggtgaag
    66661 agccatttat cgtgccgttg gcaattccgt tagtcgccgg gccgactatt ctcgccacgc
    66721 tgatgttgct gtctcatcag tacccgaatc agatggggca tctggtgatt gctctgctgc
    66781 tggcctgggg cggcaccttt gtcatcctgc tacagtcttc gctattttta cgtctgctgg
    66841 gcgagaaagg ggtgaacgca cttgaacgcc tgatgggatt gattctggtg atgatggcaa
    66901 cccagatgtt cctcgacggc attcgaatgt ggatgaaggg gtaacgagga gagtttttcg
    66961 aaaagaaccc ggtagcgtca caactaccgg gcgaacaaac ttaactcaac agctggaacg
    67021 caatcatccc aacgatggca ccgacagtgc cgaggatggt ttccatcatg gtccaggttt
    67081 tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa caaccagaaa ccggcgtcgt
    67141 taacgtggct gacaacaatc gaaccaccag cgatacaaat cgacagcgcc gccatttgcg
    67201 caccggagta gttcagttgt tcaataaccg gcatcaccag tcctaccgcc gttaaacagg
    67261 ctacggtggc agaaccctga atgatgcgca ctgcagctgc cagcacgaag caggtgatag
    67321 caatcggcag gcccatgccg gttaacgctt cgcccagtgc cggacctacg ccagagtcaa
    67381 ccagcacctg tttgaacacg ccgcctgcac caatcaccag cagaatgatc cccgccggtt
    67441 gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg catgccctga cgcattgcca
    67501 ggccataaat cgctaccaga caagcaacca gaatcgcggt aaacggatga ccaataaact
    67561 cgaaccattc gtaagcggta gagccttctg gcacaaaacg cgcggcaatg gttttcagcc
    67621 ccaccagcac cagcggcaac aggatcagcg acaggctgaa tccgaaagat ggcattttgc
    67681 cttcgccgag atgcggttcg ctgatgtcgt caggaatatg caactccacg tagcggctga
    67741 tgaaattacc ccacagcggc ccggcaataa tcattcccgg aattgccgca cacaggccaa
    67801 tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc cagcagcatt ggcgctggcc
    67861 ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa taatgggatt accagcttca
    67921 ccaggttcgt accggtgtgg cgcgccattg agaaagcaac gctaatcagc agaacaatcg
    67981 ccacttcaaa gaacagcggt agcgcacaga ccagccccgc aaggccgatg gcataatgcg
    68041 cgcggctgtg accgaaggat ttaagcattt tgacggcaat ctgatcgact gcgccggttt
    68101 catgtaagat cttgccaaac atagctccca gggcgacaac caccgccagg aagccgaggg
    68161 tgcctcccat ccctttttcc atcgtcgctg cgattttatc gagcggcata ccggaaaaaa
    68221 ggccagcccc catggacacc accatcaaag ccaggaaagc gtgcatacgc gccttcatga
    68281 ctaaaaacag cagcagtaaa acagacccta ctgctgttaa aacaagcgtt aatgtagtca
    68341 ctacttattt gcctttttta ataacctcaa tggtgcttgc cacaacacct tccagcggtt
    68401 gatcgatatc caccaccagt acatcagttt cgtccgcacc cggctcctgc agcgtttcaa
    68461 actgcgtcac caacatttgg gttttaaaga aatggccttt gcgcgctttc aggcggcttt
    68521 caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag attcggatta ccttcacgca
    68581 gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat cagtgacact ttattagtgc
    68641 gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca cggtttgcga tcgtcgtcat
    68701 tcagcggttc gccagacgcc attttttcga tattgcgccg tggatggagg aaatcgccat
    68761 caagaaacgc ggcatgaagt tgatgcgcca cttcactggc gaccgcagat ttgccgctgc
    68821 ccgatacgcc catcaagacg taaatgtggt gatcatggtt agtcgtgctc aaagtggtgc
    68881 ccccacaata caagaattaa aaatgttacg ggtaactgtt atcggtaaca ttgtccagcc
    68941 ggacaatatc agaagcaata tccatccgtg ccttaagtgt ataagtgtga gctacttcaa
    69001 atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa acctaaatct aacattttcg
    69061 gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc agcgccaata ctgcccatcc
    69121 gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat cacctggcca atgtcatgac
    69181 cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa tcccagacgc tgacattcaa
    69241 acgccgcgcc gaccgccagg tcatcgttgg tacagaacac gccatccagc tgcggatatt
    69301 cccgccgcgc ctggcgaatc agttcaatac cggaagagta agaagaagat tgctcaacca
    69361 tcacgctata cggcaccaga cctgcatcca gcattgcctg ttcgtatccc ttctgtttga
    69421 tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat gtggcaatgc ccgcgagcaa
    69481 taatggcggt ggtcatctgg cgtgccgctt caaagttatc aaaaccgacg gcgatatcga
    69541 ggcacggcga ctggctgtcc atgagttcca ccaccgggat ccccgccact tcaatcatct
    69601 ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag tccgtcgata ttccatgaga
    69661 gcatggattc gagacgttct tgctccattt ccggtttata accgtagtgc gccagcatgg
    69721 tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg taatacttcc gcgaaaacct
    69781 ggttggtgag agaaggtaac aggacgccaa tcgcccggct ggtggcgtta gagaggatat
    69841 cgggcgcacg attgggaata tagcccagtt catcaagagc ggcggcaatc ttgccgcgta
    69901 gagcgacgga aacctgctcc gggttgcgta aaaaacggct gaccgtcatt ttggtcacgc
    69961 ctacacggtc agccacatcc tgaagtacgg gtcttttctt tttcatcgtc ctgaaggtac
    70021 aaaagagata gattctctta gtttaacacg gacgcatcag aaccttcccg gggaaaacag
    70081 tgcttgttta caattattag atatacacta aatcaattgc atcgcattgt gctaacgacg
    70141 cggcaaaacc ccggaagcgt gcatcagcac gcgaccggag cgagcgagga agcctgcaaa
    70201 aaggcaggct tcaaaaatgc cgtggagctt taaaccggcg gcagatcgaa cagtaacact
    70261 tcgctgtcgc tatccgcatg gatggagatt gcctgctcat cccagattgc cagaccatcg
    70321 ctggtcgagg ctttcacgcc gttaatggtg acattgcctt tcaccacctg gatccagacg
    70381 cggcgttcag cggcaatctg atgcaccgac tgctcatctt tcagcaacgc ccagcggtac
    70441 agttccatat cctgatgcac tttcaatgaa ccatctcgcg catccggcga gagcaccagc
    70501 tgtttgccct gtacggcatc gaagcgacgc tgttcataac gcggcgtaat accgttttct
    70561 tcaggcatga tccagatctg atacagatgc agacgctcgg tgctgcttgg gttgtactct
    70621 gagtgacgaa tacccgtacc cgcactcata atctggaact cacccgccgg aacctgctct
    70681 ttattgccca tgctgtcctg atgctcaaca gtaccttcca gcacgtaggt caaaatttcc
    70741 atatctttat gcgggtgagt gccgaagccc tgccctgctt caatcacgtc gtcgttaatc
    70801 acgcgcagcg cggagaagcc cataaagttc ggatcgtaat agttggcaaa agagaaagta
    70861 tgccaggagt ccagccagcc atgatttgca tgaccacgtt catttgcttt gcgtaagtag
    70921 atcatttgta ttcgccccct gaatgatttc gatgggctta gtgtggaccc gatctgcctg
    70981 ggatgataga gggtgaaaat tgacccctct gttcaaaaaa attgaactat tcgaggggcc
    71041 atcaaactta attcgcgaga gtcactgtgg agggagaggc ttgctcaaat ccgcgttcaa
    71101 ggatttccag attggtaaga acttcagatt ccttgacgta atttggcgca ccgtgggtga
    71161 tggtttgata caactcatca taaacgcgcc cgtaatcgcc catctccggc ttcatctctt
    71221 ctctgaccgt cacgccctcg tcattgacat actccagcac accgaccgaa ttatccgctg
    71281 cgaatcccgg ttcgcccggc ataatattag ccttcaggct ggtttcctgc tggtcgatac
    71341 cgtatttaat aaacgaacct ttcttaccgt gaacgataaa tttcggataa tcgattttca
    71401 ccagatggct ggttttgacg atggctttca ggtcgccata aaacagttgc gcttcaaagg
    71461 tgtcgtcagg attggcttta ttacgcaggc tgcggatgtc ataagcgacg tgatccgggc
    71521 gaccgaacag agaaataatc tggtccatcg tatgcacacc aaggccataa aacgcgccat
    71581 cctgcggcag cccaggtttg gtctctgcca ccgggcggta atagtcaaaa tggctttcca
    71641 cttcaacaat ctctcccaac ttgccacttt caatcgcttt tttcgctgtc aggaagcagg
    71701 agtcaaagcg acgattctga tacggcgtga cggtcagccc tttgcttttc gccaacgcaa
    71761 acagctcctt cgcctgggca agcgtcgggg tgaacggttt ttcgaccagc acatttttcc
    71821 cggcttccag cgcgcgtttc gcgtactcga aatggctgtc cgcgtgggtg cagacaacaa
    71881 ccagcttaac atcgggatcg tttagtactt cgtcgagatc gctggtgaaa tggatatggg
    71941 aataaatggg agcctgttct tccggcttcg catggcgacg aaaaatatgc gcgacatgcc
    72001 agctatcctt gcggttaagt acatacggca gatggtaacg ggtggtgctt ttgccgaagc
    72061 caataaaggc gcagttgatg accatgattt cgtccttttt aaggtggtta tttacacctt
    72121 agcgcaaagc ggacgtggtt cctactggag tgcgcataac gaacacaagc acttcccgag
    72181 gataaattga gaacgaaaga tcaaaaaaaa agccagcacc cggctggcta agtaatactg
    72241 gaagcaatgt gagcaatgtc gtgctttcag gttctccgcg agggtcttcc tgatcgcgag
    72301 acaataataa tcattctcat tcgcacttgt ccaacacttt ttgcaaaaaa atgcatttga
    72361 ctcgcatttg aaagtcaatg atgttgaaag ggacatttac cccaaagagg acaaaggaat
    72421 gagtgagata gtaatacgcc acgcagaaac acgggattac gaggccatca ggcagattca
    72481 cgcccagccg gaggtgtatt gcaacacact acaggtgcct catccttccg atcatatgtg
    72541 gcaggagcga ctcgccgatc gtcccggcat caagcaactc gtcgcctgta ttgatggaga
    72601 cgtcgtgggc catctcacca ttgacgtgca acagcgccca cgccgcagtc atgttgccga
    72661 ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc gtcgccagcg ccctgatgcg
    72721 agagatgatc gaaatgtgcg acaactggtt gcgggtagat cgcattgaac taaccgtgtt
    72781 tgtcgataac gccccggcaa ttaaggtcta taaaaaatac ggctttgaaa ttgaagggac
    72841 tggtaagaag tacgcattgc gtaatggtga atatgtcgat gcatattata tggcgcgggt
    72901 gaagtaagat agtgcccttt ttctgagatg gaaaaagggt gtcattcaaa atcgacatac
    72961 cttcctttaa ggtatttatt tgcccaatac atatattgag atttaactca taattacttc
    73021 ctaaagtgta a
//
LOCUS       DAPGWD010000004        70029 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPGWD010000004 DAPGWD010000000
VERSION     DAPGWD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.004"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..139
                     /locus_tag="RHX88_000210"
     CDS             <1..139
                     /locus_tag="RHX88_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDV2228945.1"
                     /translation="DRLYQTVGQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWPQ
                     E"
     gene            complement(282..1466)
                     /gene="araJ"
                     /locus_tag="RHX88_000211"
     CDS             complement(282..1466)
                     /gene="araJ"
                     /locus_tag="RHX88_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDV2228946.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGWISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(1592..4735)
                     /gene="sbcC"
                     /locus_tag="RHX88_000212"
     CDS             complement(1592..4735)
                     /gene="sbcC"
                     /locus_tag="RHX88_000212"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDV2228947.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(4732..5934)
                     /gene="sbcD"
                     /locus_tag="RHX88_000213"
     CDS             complement(4732..5934)
                     /gene="sbcD"
                     /locus_tag="RHX88_000213"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDV2228948.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            6124..6813
                     /gene="phoB"
                     /locus_tag="RHX88_000214"
     CDS             6124..6813
                     /gene="phoB"
                     /locus_tag="RHX88_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDV2228949.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6871..8166
                     /gene="phoR"
                     /locus_tag="RHX88_000215"
     CDS             6871..8166
                     /gene="phoR"
                     /locus_tag="RHX88_000215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDV2228950.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            8573..9892
                     /gene="brnQ"
                     /locus_tag="RHX88_000216"
     CDS             8573..9892
                     /gene="brnQ"
                     /locus_tag="RHX88_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDV2228951.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            9968..11341
                     /gene="proY"
                     /locus_tag="RHX88_000217"
     CDS             9968..11341
                     /gene="proY"
                     /locus_tag="RHX88_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDV2228952.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            11497..13314
                     /gene="malZ"
                     /locus_tag="RHX88_000218"
     CDS             11497..13314
                     /gene="malZ"
                     /locus_tag="RHX88_000218"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDV2228953.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCRVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(13319..13900)
                     /gene="acpH"
                     /locus_tag="RHX88_000219"
     CDS             complement(13319..13900)
                     /gene="acpH"
                     /locus_tag="RHX88_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDV2228954.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            13993..15063
                     /gene="queA"
                     /locus_tag="RHX88_000220"
     CDS             13993..15063
                     /gene="queA"
                     /locus_tag="RHX88_000220"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDV2228955.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            15119..16246
                     /gene="tgt"
                     /locus_tag="RHX88_000221"
     CDS             15119..16246
                     /gene="tgt"
                     /locus_tag="RHX88_000221"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDV2228956.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            16269..16601
                     /gene="yajC"
                     /locus_tag="RHX88_000222"
     CDS             16269..16601
                     /gene="yajC"
                     /locus_tag="RHX88_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDV2228957.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            16629..18476
                     /gene="secD"
                     /locus_tag="RHX88_000223"
     CDS             16629..18476
                     /gene="secD"
                     /locus_tag="RHX88_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDV2228958.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            18487..19458
                     /gene="secF"
                     /locus_tag="RHX88_000224"
     CDS             18487..19458
                     /gene="secF"
                     /locus_tag="RHX88_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDV2228959.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            19587..19934
                     /locus_tag="RHX88_000225"
     CDS             19587..19934
                     /locus_tag="RHX88_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDV2228960.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(20111..20995)
                     /gene="tsx"
                     /locus_tag="RHX88_000226"
     CDS             complement(20111..20995)
                     /gene="tsx"
                     /locus_tag="RHX88_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDV2228961.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(21294..21833)
                     /locus_tag="RHX88_000227"
     CDS             complement(21294..21833)
                     /locus_tag="RHX88_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV2228962.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            21984..22433
                     /gene="nrdR"
                     /locus_tag="RHX88_000228"
     CDS             21984..22433
                     /gene="nrdR"
                     /locus_tag="RHX88_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDV2228963.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            22437..23540
                     /gene="ribD"
                     /locus_tag="RHX88_000229"
     CDS             22437..23540
                     /gene="ribD"
                     /locus_tag="RHX88_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDV2228964.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            23629..24099
                     /gene="ribE"
                     /locus_tag="RHX88_000230"
     CDS             23629..24099
                     /gene="ribE"
                     /locus_tag="RHX88_000230"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDV2228965.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            24119..24538
                     /gene="nusB"
                     /locus_tag="RHX88_000231"
     CDS             24119..24538
                     /gene="nusB"
                     /locus_tag="RHX88_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDV2228966.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            24616..25593
                     /gene="thiL"
                     /locus_tag="RHX88_000232"
     CDS             24616..25593
                     /gene="thiL"
                     /locus_tag="RHX88_000232"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDV2228967.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            25571..26089
                     /gene="pgpA"
                     /locus_tag="RHX88_000233"
     CDS             25571..26089
                     /gene="pgpA"
                     /locus_tag="RHX88_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDV2228968.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(26143..27117)
                     /gene="yajO"
                     /locus_tag="RHX88_000234"
     CDS             complement(26143..27117)
                     /gene="yajO"
                     /locus_tag="RHX88_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDV2228969.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(27297..29159)
                     /gene="dxs"
                     /locus_tag="RHX88_000235"
     CDS             complement(27297..29159)
                     /gene="dxs"
                     /locus_tag="RHX88_000235"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDV2228970.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIDTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEDKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(29184..30083)
                     /gene="ispA"
                     /locus_tag="RHX88_000236"
     CDS             complement(29184..30083)
                     /gene="ispA"
                     /locus_tag="RHX88_000236"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDV2228971.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(30083..30325)
                     /gene="xseB"
                     /locus_tag="RHX88_000237"
     CDS             complement(30083..30325)
                     /gene="xseB"
                     /locus_tag="RHX88_000237"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDV2228972.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            30531..31979
                     /gene="thiI"
                     /locus_tag="RHX88_000238"
     CDS             30531..31979
                     /gene="thiI"
                     /locus_tag="RHX88_000238"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDV2228973.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(32033..32623)
                     /gene="yajL"
                     /locus_tag="RHX88_000239"
     CDS             complement(32033..32623)
                     /gene="yajL"
                     /locus_tag="RHX88_000239"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDV2228974.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPAIVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(32586..33497)
                     /gene="panE"
                     /locus_tag="RHX88_000240"
     CDS             complement(32586..33497)
                     /gene="panE"
                     /locus_tag="RHX88_000240"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDV2228975.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVATPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            33665..34156
                     /gene="yajQ"
                     /locus_tag="RHX88_000241"
     CDS             33665..34156
                     /gene="yajQ"
                     /locus_tag="RHX88_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDV2228976.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(34284..35648)
                     /locus_tag="RHX88_000242"
     CDS             complement(34284..35648)
                     /locus_tag="RHX88_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2228977.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(35797..36687)
                     /gene="cyoE"
                     /locus_tag="RHX88_000243"
     CDS             complement(35797..36687)
                     /gene="cyoE"
                     /locus_tag="RHX88_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDV2228978.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(36699..37028)
                     /locus_tag="RHX88_000244"
     CDS             complement(36699..37028)
                     /locus_tag="RHX88_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDV2228979.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(37028..37642)
                     /locus_tag="RHX88_000245"
     CDS             complement(37028..37642)
                     /locus_tag="RHX88_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDV2228980.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(37632..39623)
                     /gene="cyoB"
                     /locus_tag="RHX88_000246"
     CDS             complement(37632..39623)
                     /gene="cyoB"
                     /locus_tag="RHX88_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDV2228981.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(39645..40592)
                     /gene="cyoA"
                     /locus_tag="RHX88_000247"
     CDS             complement(39645..40592)
                     /gene="cyoA"
                     /locus_tag="RHX88_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDV2228982.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(41052..42527)
                     /gene="ampG"
                     /locus_tag="RHX88_000248"
     CDS             complement(41052..42527)
                     /gene="ampG"
                     /locus_tag="RHX88_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDV2228983.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(42571..43149)
                     /locus_tag="RHX88_000249"
     CDS             complement(42571..43149)
                     /locus_tag="RHX88_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2228984.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            43454..43771
                     /gene="bolA"
                     /locus_tag="RHX88_000250"
     CDS             43454..43771
                     /gene="bolA"
                     /locus_tag="RHX88_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDV2228985.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            44115..45413
                     /gene="tig"
                     /locus_tag="RHX88_000251"
     CDS             44115..45413
                     /gene="tig"
                     /locus_tag="RHX88_000251"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDV2228986.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            45659..46282
                     /gene="clpP"
                     /locus_tag="RHX88_000252"
     CDS             45659..46282
                     /gene="clpP"
                     /locus_tag="RHX88_000252"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDV2228987.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            46408..47682
                     /gene="clpX"
                     /locus_tag="RHX88_000253"
     CDS             46408..47682
                     /gene="clpX"
                     /locus_tag="RHX88_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDV2228988.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            47870..50224
                     /gene="lon"
                     /locus_tag="RHX88_000254"
     CDS             47870..50224
                     /gene="lon"
                     /locus_tag="RHX88_000254"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDV2228989.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            50433..50705
                     /gene="hupB"
                     /locus_tag="RHX88_000255"
     CDS             50433..50705
                     /gene="hupB"
                     /locus_tag="RHX88_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDV2228990.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            50897..52768
                     /gene="ppiD"
                     /locus_tag="RHX88_000256"
     CDS             50897..52768
                     /gene="ppiD"
                     /locus_tag="RHX88_000256"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV2228991.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            52919..53290
                     /locus_tag="RHX88_000257"
     CDS             52919..53290
                     /locus_tag="RHX88_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDV2228992.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            53384..53782
                     /gene="fadM"
                     /locus_tag="RHX88_000258"
     CDS             53384..53782
                     /gene="fadM"
                     /locus_tag="RHX88_000258"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDV2228993.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(53834..54529)
                     /gene="queC"
                     /locus_tag="RHX88_000259"
     CDS             complement(53834..54529)
                     /gene="queC"
                     /locus_tag="RHX88_000259"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDV2228994.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(54594..56294)
                     /locus_tag="RHX88_000260"
     CDS             complement(54594..56294)
                     /locus_tag="RHX88_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDV2228995.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            56394..57212
                     /gene="cof"
                     /locus_tag="RHX88_000261"
     CDS             56394..57212
                     /gene="cof"
                     /locus_tag="RHX88_000261"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDV2228996.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            57365..57823
                     /gene="decR"
                     /locus_tag="RHX88_000262"
     CDS             57365..57823
                     /gene="decR"
                     /locus_tag="RHX88_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDV2228997.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            57853..59625
                     /locus_tag="RHX88_000263"
     CDS             57853..59625
                     /locus_tag="RHX88_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV2228998.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            59618..61399
                     /locus_tag="RHX88_000264"
     CDS             59618..61399
                     /locus_tag="RHX88_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV2228999.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDER
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            61580..61918
                     /gene="glnK"
                     /locus_tag="RHX88_000265"
     CDS             61580..61918
                     /gene="glnK"
                     /locus_tag="RHX88_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDV2229000.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            61948..63234
                     /gene="amtB"
                     /locus_tag="RHX88_000266"
     CDS             61948..63234
                     /gene="amtB"
                     /locus_tag="RHX88_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDV2229001.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(63283..64143)
                     /gene="tesB"
                     /locus_tag="RHX88_000267"
     CDS             complement(63283..64143)
                     /gene="tesB"
                     /locus_tag="RHX88_000267"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDV2229002.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            64361..64933
                     /locus_tag="RHX88_000268"
     CDS             64361..64933
                     /locus_tag="RHX88_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2229003.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(64964..65275)
                     /locus_tag="RHX88_000269"
     CDS             complement(64964..65275)
                     /locus_tag="RHX88_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDV2229004.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            65438..65534
                     /gene="ffs"
                     /locus_tag="RHX88_000270"
     ncRNA           65438..65534
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RHX88_000270"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            65654..66007
                     /locus_tag="RHX88_000271"
     CDS             65654..66007
                     /locus_tag="RHX88_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HDV2229005.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(66049..67599)
                     /gene="pdeB"
                     /locus_tag="RHX88_000272"
     CDS             complement(66049..67599)
                     /gene="pdeB"
                     /locus_tag="RHX88_000272"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDV2229006.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(67763..68233)
                     /locus_tag="RHX88_000273"
     CDS             complement(67763..68233)
                     /locus_tag="RHX88_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDV2229007.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(68349..68900)
                     /gene="maa"
                     /locus_tag="RHX88_000274"
     CDS             complement(68349..68900)
                     /gene="maa"
                     /locus_tag="RHX88_000274"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDV2229008.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPNNVVVGGNPARIIKKL"
     gene            complement(69072..69290)
                     /gene="hha"
                     /locus_tag="RHX88_000275"
     CDS             complement(69072..69290)
                     /gene="hha"
                     /locus_tag="RHX88_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDV2229009.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(69316..69690)
                     /gene="tomB"
                     /locus_tag="RHX88_000276"
     CDS             complement(69316..69690)
                     /gene="tomB"
                     /locus_tag="RHX88_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDV2229010.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        16892 a        17464 c        18782 g        16891 t
ORIGIN      
        1 agaccgttta tatcaaacgg ttgggcagtt gattaaacaa tttgtcttcg gcggcgaatg
       61 tgaaacgccg gtgcgtaagg cgaagcacgg tgattccagc ggcgtacgcg gcgctgcgtg
      121 gttatggcca caagagtaaa aaacgtaggc aattggcgca tcatgcctga tgcgacgctt
      181 gccgcgtctt atcaggccta caaaatgtgc cagaaccgcg tagggcggat aaggcgttca
      241 cgccgcatcc ggcaataagc aacgcgcacc gtgacagtaa tctaccccag tggtttcgcc
      301 agcaccggac tatccgccgc ttgctggcgc ttatagcgac catacagcag caacgatgac
      361 atcgcagcaa acgaaagcag ggcggcaggc agcgccacgt aattatatgc cagccccagc
      421 gtcagcatca taccaccgca atatgcgccg acggcgctac cgaggttaaa cgctatttgc
      481 ccacctgcgg cacctaataa ctctccgcct ttggcatttt gtagtaataa tatttgcaga
      541 ggtgctgaaa gggcaaataa tcccgcgcaa caaataaaag caaaaataag cgacgttgtt
      601 ttcatgccgc cgcagaaaaa gagcatcagc agtgccagta caattataaa gtcagtcact
      661 gctgcaatgc gcagtggtga ataacgtcct gaaatccagc cacttagcat atttcccaac
      721 accatcccta gcccaactaa catcataata aaggtcatcg ccgtttccga aaaaccggaa
      781 ataaacatca tgtatggctt tacgtagctg aaccaggcaa acacacctgc gttgccaaac
      841 atggtggcgg cgaaaattaa ccacggggcc gggctgcgca aaaagtgaaa ttgttcgcgc
      901 agctttcctt tcgcctcgtc gcgaatatcc ggcacccaaa aatagaccga tgccatcacc
      961 gcaatattaa aaacagcgat caataaaaag gtgtaacgcc agctaaattc ctgacttaaa
     1021 tacgttccca gcggaatgcc cagcaaattg gcgactgtca tcccggaaac catccccgcc
     1081 acggcggcgg tgacttttcc gggtttgata atttttgata acacgatcgc tccgacgcca
     1141 aaaaatgcgc catgcggaaa gccggatacc agccgaccaa tggcgagcat caggtaagac
     1201 gaagagagcg tgaacatggc gttgccaatg acgcacaacg ccaccagaaa caacaagata
     1261 tgtttgagtg agtagcggct ggaaaagagt gcgatgattg gcgcaccgac caccaccccc
     1321 agtgcataat acgagatcat atgcccggca gcaggaatcg aaattcctac gttatgcgcc
     1381 agctccgtga gcacgcccat aatgccaaat tcggccatcc ccaaaccaaa cgtgcccaga
     1441 gccaaagata aaatgacttt tttcatacca ccatcacaac caccagaacg gctaccaatc
     1501 accgtagcca ataaagtgat agcttgcagg ataacgacca gcaaggccaa gagataattc
     1561 gcccctctgt attcattatc ctgctgaata gttatttcac tgcaaacgta ctttccagtt
     1621 tgctgtagcc caggccgttg atctttttca ctttgatctg caccggaata cgctctttca
     1681 tcgcttctac gtggctaatc acaccgatgg ttttgccact ggcgttcagg gcatccagcg
     1741 catcaagggc ggtatccagc gtttcgctat ccagcgtgcc aaaaccttca tcaaggaaca
     1801 gcgagtcaat acgtgtttta tggctgacca gatccgaaag cgccagcgcc agcgccagac
     1861 taacgaggaa actttcgccg ccggaaaggg tacgggtatc gcgtaccgca tctgcctgcc
     1921 aggtatcaac aacctcgact tccagcgcct cgctggcttt gcgctgtaac agatagcgcc
     1981 cgtgcagccg ggtaagttgc tgattagcga gatggactaa attatccagc gtcagcccct
     2041 gggcaaactt gcggaattta tcgccctctt tggaacctat tagcgaattc agatatcccc
     2101 agtcctcaac ctgctgcgtc atttgagcaa tttgctgcat taaggtttgt tgttgctgac
     2161 ggttatctgc atcctgcttc agctgctggc gaatctcgcc ttgactcgtg gtgttttcac
     2221 gcaacttttg gtgagtttgc gctaactctt gctgaatctg ctccaccgtc acagtgagag
     2281 ccaacccgtc aggtcggtgt tgttgatgct gtgccagcgt ttctgctgtc tgagtgacca
     2341 gcgtttgcgc ctgacggcgc tggttttcca gattctgctt gagctgttcc agctgcgtta
     2401 gtgtttgttc atccattagc gccgcaagga acgcctgctg atcgtcaaag acgctggcct
     2461 gtagcgcggt gtcaaactgc gcctgggctt tttgcagact ttgcgccgcc agaacatcct
     2521 gttgctgtaa cgtctgctgc tggctgtgta atgcgagaca ttgttcatga acctgccgcc
     2581 agttatccag cgccacagtt tcttcgctgt gcgggagatc atcactttgc ggcaacgttt
     2641 ccagaatcgg cgtcagctgc tgaatacggt tttgcagcgc ggttaattcg ttctggcgtt
     2701 gctgccagct ctgcgcttct tgctgacgtg tcgccaacca gctctcttct tcatcttcct
     2761 gtggcaatgt cagtgcataa cccgccaatg ccgttaagag ctgctgctgg cgttgttcaa
     2821 tttgctgttg atactggata atttgctgat tatgcgcggc aatctgccct tgtaattcat
     2881 gtcgttggct gagtaaccgc agctggcgtt cgtgctcatc ttgtgcatcc agccacggtt
     2941 gaatatcgtc ctgtggctgc aaggtgatat tgaggctggc cgtgacggct tgccattgtt
     3001 gagtaagtgc ttgctcatct tgtcggaggc tttgcgcttc gttttcatcg cgctgaagct
     3061 gctttgttaa ggcgtccagt tgcccgcgca gcgccgcacc ttcttcgccg agctttttaa
     3121 cttcgttttc cagcgccagt aatcgagact gattaacacc aggctccagc gcctgatacg
     3181 cctcgaccgc cgggtggctg gtggaaccac aaagtggaca aggctgaccc gcctgtaact
     3241 gtgcacgttg agcttccagc gttttgatgc gcgcttcctg ctcgcaaatg gttttcacat
     3301 cggcaagttg ctgcgtcttt tctttataac gctggcgcat ttcgttaagt gcggcgttac
     3361 gttgcgtctg ttcttgcgtg acattctgga tagcgacctg taactgcgcc agacgttttt
     3421 gttggggaac aatctgtcca tgtagcgcga ccaggtgctg acgcagtggg cgttgctcag
     3481 catgttgcgc cagggcggta gcaacttcat cggcggttaa cgtcaacgtg atcgccgcaa
     3541 gcgcattaag tttttgctca gcatgggtta actgttgctg ccattgccgc agatgctcgc
     3601 gatcgctggt ttgttgggag aactgcgcac gccaacccgc cagttcgttg ttccactgac
     3661 ggaagcggtc gtgttcctgt aaccaggtat tcaggctttg ttgctgctgc tgtaattctg
     3721 ctgactgctt cgccgcgtgg tggcgaatgc tcgcgcgaag cgccattgtg ctctgtaagc
     3781 gagtatttac ttcttcaatc tgctggcgag tatgcgccag cgccgcgctg tgttctgcga
     3841 tgcgttccca gtgcggacga agatttcgtg ccggttgtgc cagactgagc gccgccagtt
     3901 gaggttgcgc tttttcttct tcggctaacg cctgttgcaa ggcctgctga cggcggctgg
     3961 cttcttgctg caattcgtcc agacgcgtta accagtttag tgattgttgt tcttgctgct
     4021 gcgcggtaag taactgtttt tcttcgtcag taagtacctg caaactcgct gtcagcgatt
     4081 gcacttgttc cggcgtgagc aacgcgacgc cgctggcctg cgcttgcagc ttctccagct
     4141 ctgtgcgggc cgatttgtgc tgctcaaaaa ccatcgccga gatttgcccg tagatttcag
     4201 tgccggttaa ctcctcgagc aattccgcgc gttctttggg tttggcattc aggaaggcag
     4261 caaattgccc ctgcgaaagc agcatcgaac gggtgaagcg cccgtaatcc agcccggtta
     4321 acgtcgctgt cagttccagc ttatctttca ctttgtcggc gagaattttg ccgtcggcgc
     4381 agcgcgccag ctctacgcgt ggcacctgca aattaccgtc gggttggtta cgcgcccgat
     4441 tctggctcca gaatgcacgg tacgcttcac ctttcacttc aaactccacc tccgccagac
     4501 attcggcggt atcgcgggtc atgagatcat tttgcgattg tgaaacgtta gagagacgcg
     4561 gagtttcgtg atacagcgcc agacaaatgg cgtccagcag ggtggttttc cccgcacctg
     4621 ttgggccggt aatagcaaac agcccgttgc tggcgaacgg ctcgcgggtg aaatcaatct
     4681 tccattcgcc ttttaatgag ttcaggtttt tcaggcgcag gctgagaatt ttcatgcttc
     4741 gtgttctccg gcgagggtat gcaacgtcgt ggtgaaaaga tgctgcagac gttgctgctg
     4801 cgattcatcc agttcttcca gtgccagacg gcgattgaac acctcttcga cgctgagttc
     4861 gctgagggtt tcacgctgtt ggctggctaa cacacgctcg cgctgttcac gactccgacg
     4921 taccagcaat acttcgacag gcaatgattc ggttaatgcc tggattttgc gctgaatatc
     4981 atgcagatac tcatcagtag tgatttcgat atccagccag acaggtggct cctgcgatac
     5041 atcgcgccac tgttccagct gtgcggtaat cgacgccaga tcgcctttca gcactgccat
     5101 gggttgcgtt accggtacgt tcaggttttc cacgctctct aatttgccgt ttgaaaatgt
     5161 caccagatgg acatatttac tcttaccgca ttcatcaaaa ctcagtggaa tgggggagcc
     5221 gcaatagcga acatgttcca tgccgccaat aatctgtgcg cggtgaatat gcccgagcgc
     5281 gatgtagtcg gctggtggaa agttttgtgc cggaaacgcg tccagcgtgc caatataaat
     5341 gtcacgcacg gcgtcacttt tactggcccc cacggtcgtt aaatgtcccg tggcgatgat
     5401 gggcagaggc tgatcgccgc gcagtttgca ggcatcggca tagtgttgtt ggtaataatc
     5461 ggtaatcgct gccagtaaat gctgctgttt ttcaataccg ttaagccccg cctggctggt
     5521 aataatgtca cgtggacgta aaaacggaat ggggcacagc actgcgcctg gcgtcccgtc
     5581 gcgacgaggc aagatttgcg gcgcatgtcc ggcgctggcg accacggtag tattgaggaa
     5641 cgccatgata tcgcgcgatt cattcagcgt ggcgaccgag tcatggtttc ctgccagtac
     5701 caccagatga cagccagttt gctgtaaatt gacaacaaaa cggttgtata acgtgcgggc
     5761 gtaactgggc ggcgagccgg tatcgaaaac atcaccagca acaataatcg catccacctg
     5821 atgggtttgt gctgtctcca gcagccagtc aagaaaagcc tgatgttcag cttcgcggct
     5881 tttactgtag aagttctggc cgagatgcca gtctgaggtg tgaaggatgc gcataacggt
     5941 tccctggcga aaaagcatgg gcgcgattat acccaaacag atgtgccatt tgcttttttc
     6001 tgcgccacgg aaatcaataa cctgaagata tgtgcgacga gcttttcata aatctgtcat
     6061 aaatctgacg cataatgacg tcgcattaat gatcgcaacc tatttattac aacagggcaa
     6121 atcatggcga gacgtattct ggtcgtagaa gatgaagctc caattcgcga aatggtctgc
     6181 ttcgtgctcg aacaaaatgg ctttcagccg gtcgaagcgg aagattatga cagtgctgtg
     6241 aatcaactga atgaaccctg gccggattta attctccttg actggatgtt acctggcggc
     6301 tccggtatcc agttcatcaa acacctcaag cgcgagtcga tgacccggga tattccagtg
     6361 gtgatgttga ccgccagagg ggaagaagaa gatcgcgtgc gcggccttga aaccggcgcg
     6421 gatgactata tcaccaagcc gttttcgccg aaggagctgg tggcgcgaat caaagcggta
     6481 atgcgccgta tttcgccaat ggcggtggaa gaggtgattg agatgcaggg attaagtctc
     6541 gacccgacat ctcaccgagt gatggcgggc gaagagccgc tggagatggg gccgacagaa
     6601 tttaaactgc tgcacttttt tatgacgcat cctgagcgcg tgtacagccg cgagcagctg
     6661 ttaaaccacg tctggggaac taacgtgtat gtggaagacc gcacggtcga tgtccacatt
     6721 cgtcgcctgc gtaaagcact ggagcccggc gggcatgacc gcatggtgca gaccgtgcgc
     6781 ggtacaggat atcgtttttc aacccgcttt taacgccttg ctcatcggac gcagagcagg
     6841 gcttatgatt tcttaactgg agtatcttac gtgctggaac ggctgtcgtg gaaaaggctg
     6901 gtgctggagc tgctactttg ctgcttcccg gctttcatcc tgggtgcatt ttttggttac
     6961 ctgccctggt ttttgctggc atcggtaacg ggactgctta tctggcattt ctggaattta
     7021 ttgcgccttt catggtggct gtgggtggat cgtagtatga ccccgccacc ggggcgtggt
     7081 agctgggaac cgctactata cggcttacac cagatgcagc tgcgaaataa aaaacgccgc
     7141 cgtgaactgg gcaatctgat taaacgcttt cgtagcggcg cggagtcgct gcccgacgca
     7201 gtggtgctga ccactgaaga gggcggtatt ttctggtgta acggtctggc gcaacaaatt
     7261 cttggtttgc gctggccgga agataacggg cagaacatcc ttaacctgct gcgttacccg
     7321 gagtttacgc aatatctgaa aacgcgtgat ttttctcgcc cgctcaatct ggtgctcaac
     7381 accgggcggc atctggaaat tcgcgtcatg ccttataccc acaaacagtt gctgatggtg
     7441 gcgcgtgatg tcacgcaaat gcatcaactg gaaggggcgc ggcgtaactt ttttgccaac
     7501 gtgagccatg agttacgcac gccattgacc gtgttacagg gttacctgga gatgatggat
     7561 gagcagccgc tggaaggtgc ggtacgcgaa aaagcgttgc acaccatgcg cgagcagacc
     7621 cagcggatgg aagggctggt gaagcagttg ctgacgctgt cgaaaataga agccgcaccg
     7681 acgcatttgc tcaatgaaaa ggttgatgtg ccgatgatgc tgcgcgttgt tgagcgtgag
     7741 gctcagactc tgagtcagaa aaaacagaca tttacctttg agatagataa cggcctcaaa
     7801 gtgtctggca acgaagatca gctacgcagt gcgatttcga acctggtcta taacgccgtg
     7861 aatcatactc cagaaggcac gcatatcacc gtacgctggc agcgagtgcc gcacggtgcc
     7921 gaatttagcg ttgaagataa cggaccgggc attgcaccgg agcatattcc gcgcctgacc
     7981 gagcgttttt atcgcgttga taaagcgcgt tcccggcaaa ccggcggtag cggattaggg
     8041 ttagcgatcg tgaaacatgc tgtgaatcat cacgaaagtc gcctgaatat tgagagtaca
     8101 gtaggaaaag gaacacgttt cagttttgtt atcccggaac gtttaattgc caaaaacagc
     8161 gattaatccg cctttgtcat cttttattgc cataagccag tcgatgctgg cttattttct
     8221 ttgcagtcaa aatacgggcg ttagatttta caacggttgg tgattttttg ttcgcatgat
     8281 tagccatgtc tttttcacgg aaatagtgtt ttatactggt tggtgatttc ttatcgctat
     8341 atacctctgg tttttagatc cctccttgct ttaaaacgtt ataagcgttt aaattgcgct
     8401 tcaggtgctg tcatactgac tgcattaacg cggtaaatcg aaaaactatt cttcgccgcg
     8461 cctggttggg agtatttccc gctaaaattg tttaaatata ccgctgtatc atccccaggg
     8521 attggcacaa aaatttaacg ttacaacacc acatccacag gcagtatgat ttatgaccca
     8581 tcaattaaga tcgcgcgata tcatcgctct gggctttatg acatttgcgt tgttcgtcgg
     8641 cgcaggtaac attattttcc ctccaatggt cggcttgcag gcaggcgaac acgtctggac
     8701 tgcggcattc ggcttcctca ttactgccgt tggtctgccg gtactaacgg tagtggcgct
     8761 ggcaaaagtt ggcggcggtg ttgacagcct cagcacgcca atcggtaaag tcgctggcgt
     8821 gctgctggca acggtttgtt acctggcggt ggggccgctt ttcgctacgc cgcgtacagc
     8881 taccgtttcc tttgaagtgg ggattgcgcc gctgacgggt gattccgcgc tgccgctgtt
     8941 tatctacagc ctggtctatt tcgctatcgt tattctggtt tcgctctatc cgggcaagct
     9001 gctggatacc gtgggcaact tccttgcgcc gctgaaaatt atcgcgctgg tcatcctgtc
     9061 tgttgccgcg attgtctggc cggcgggttc tatcagcacg gcgactgagg cttatcaaaa
     9121 cgctgcgttt tctaacggct tcgttaacgg ctatctgacc atggatacgc tgggcgcaat
     9181 ggtgtttggt atcgttattg ttaacgcggc gcgttctcgt ggcgttaccg aagcgcgtct
     9241 gctgacccgt tataccgtct gggctggcct gatggcgggt gttggtctga ctctgctgta
     9301 cctggcgctg ttccgtctgg gttcagacag cgcgtcgctg gtcgatcagt ctgcaaacgg
     9361 cgctgctatt ctgcatgctt acgttcagca cacctttggc ggcggcggta gcttcctgct
     9421 ggcggcgtta atcttcatcg cctgcctggt aacggcagtt ggcctgacct gtgcttgtgc
     9481 agaattcttt gcccagtacg taccgctctc ttatcgtacg ctggtgttta tcctcggcgg
     9541 cttctcgatg gtggtttcta acctcggctt aagccagctg atccagatct ccgtaccggt
     9601 gctgaccgct atttatccgc cgtgtatcgc actggttgta ttaagtttta cacgctcatg
     9661 gtggcataat tcgtcccgcg tgattgctcc gccgatgttt atcagcctgc tttttggtat
     9721 tctcgacggg atcaaagcat ctgcattcag cgatatctta ccgtcctggg cgcagcgttt
     9781 accgctggcc gaacaaggtc tggcgtggtt aatgccaaca gtggtgatgg tggttctggc
     9841 cattatctgg gatcgcgcgg caggtcgtca ggtgacctcc agcgctcact aaatcactga
     9901 acatttgttt taaccacggg gctgcgatgc cccgtggttt tttattgtgt tgatgggtta
     9961 ggaattgatg gaaagtaaga acaagctaaa gcgtgggcta agtacccgcc acatacgctt
    10021 tatggcactg ggttcagcaa ttggcaccgg gctgttttac ggttcggcag acgccatcaa
    10081 aatggccggt ccgagcgtgt tgttggccta tattatcggt ggtatcgcgg cgtatatcat
    10141 tatgcgtgcg ctgggggaaa tgtcggtaca taacccggca gccagctctt tctcgcgtta
    10201 tgcgcaggaa aacctcggcc cgctggcagg ttacattacc ggctggacct attgctttga
    10261 aatccttatt gtcgccatcg ccgatgtgac cgcttttggt atctatatgg gtgtctggtt
    10321 cccgacggtg ccgcactgga tttgggtact gagcgtggtg ctgatcattt gcgccgtaaa
    10381 cctgatgagc gtgaaggtct tcggtgagct ggaattctgg ttctcgttct ttaaagtcgc
    10441 caccatcatc atcatgattg tcgccggttt cggcatcatc atctggggga ttggcaacgg
    10501 cgggcaaccg accggtattc ataacctgtg gagcaacggc ggcttcttca gtaacggctg
    10561 gcttgggatg gtgatgtcgt tgcaaatggt gatgtttgct tacggtggga tcgaaattat
    10621 cgggattacc gccggtgaag cgaaagatcc tgagaaatcg ataccgcgtg cgattaactc
    10681 cgtgccgatg cgtattctgg tgttctacgt cggtacgctg ttcgtcatta tgtctatcta
    10741 cccgtggaat caggttggca ctgccggtag cccgttcgtg ctgacgttcc agcatatggg
    10801 cattaccttt gccgccagca ttcttaactt tgttgtgctg actgcttcgc tgtcggcaat
    10861 taacagtgat gtatttggcg taggccgtat gctccacggt atggcagagc agggcagcgc
    10921 gccgaaaatt ttcagcaaaa cgtcgcgtcg cggtattccg tgggttacgg tgctggtgat
    10981 gactaccgcg ctgctgtttg cggtgtatct gaactacatc atgccggaaa acgtcttcct
    11041 ggtgatcgct tcgctggcaa ccttcgccac ggtgtgggtg tggattatga tcctgctgtc
    11101 gcaaattgcc ttccgtcgcc gtttgccgcc agaagaagtt aaggcgctga aatttaaagt
    11161 gccgggtggg gtagcaacga ccatcggcgg gctgattttc ctgctcttta ttatcgggtt
    11221 gattggttat cacccggata cgcgtatctc gctgtatgtc ggtttcgcgt ggattgttgt
    11281 gctgttgatt ggctggatgt ttaaacgccg ccacgatcgt cagctggctg aaaaccaata
    11341 atacttgcgt tctgtaagcc ggttcaggag gaaatcctga tccggctatt ccgaaagtta
    11401 tccgcccccg tcctcctccc ccaaatatcc ttcagatgat gagtgatcct gcattaggct
    11461 atggcaaggt gatcagattt tcatcacagg ggaattatga tgttaaatgc atggcacctg
    11521 ccggtgcccc catttgttaa acaaagcaaa gatcaactgc tcattacact gtggctgacg
    11581 ggcgaagacc caccgcagcg cattatgctg cgtacagaac acgataacga agaaatgtca
    11641 gtatcgatgc ataagcagcg cagtcagcag cagcctggcg tcaccgcatg gcgtgcggcg
    11701 attgatctct ccagcggaca accccggcgg cgttacagtt tcaaactgct gtggcacgat
    11761 cgccagcgtt ggtttacacc gcagggcttc agccgaatgc cgccggcacg actggagcag
    11821 tttgccgtcg atgtaccgga tatcggccca caatgggctg cggatcagat tttttatcag
    11881 atcttccctg atcgttttgc gcgtagtctt cctcgtgaag ctgaacagga tcatgtctat
    11941 taccatcatg cagccggaca agagatcatc ttgcgtgact gggatgaacc ggtcacggcg
    12001 caggcgggcg gatcaacgtt ctatggcggc gatctggacg ggataagcga aaaactgccg
    12061 tatctgaaaa agcttggcgt gacggcgctg tatctcaatc cggtgtttaa agctcccagc
    12121 gtacataaat acgataccga ggattatcgc catgtcgatc cgcagtttgg cggtgatggg
    12181 gcgttgctgc gtttgcgaca caatacgcag cagctgggaa tgcggctggt gctggacggc
    12241 gtgtttaacc acagtggcga ttcccatgcc tggtttgaca ggcacaatcg tggcacgggg
    12301 ggagcttgtc acaaccctga atcgccctgg cgcgactggt actcgtttag tgatgatggc
    12361 acggcgctcg actggcttgg ctatgccagc ttgccgaagc tggattatca gtcggaaagt
    12421 ctggtgaatg aaatttatcg cggggaagac agtattgtcc gccattggct gaaagcgccg
    12481 tggagtatgg acggctggcg gctggatgtg gtgcatatgc tgggggaggc gggtggggcg
    12541 cgcaataata tgcagcacgt tgctgggatc accgaagcgg cgaaagaaac ccagccggaa
    12601 gcgtatattg tcggcgaaca ttttggcgat gcacggcaat ggttacaggc cgatgtggaa
    12661 gatgccgcca tgaactatcg tggcttcaca ttcccgttgt ggggatttct tgccaatacc
    12721 gatatctctt acgatccgca gcaaattgat gcccaaacct gtatggcctg gatggataat
    12781 taccgcgcag ggctttctca tcaacaacaa ttacgtatgt ttaatcagct cgacagccac
    12841 gatactgcgc gatttaaaac gctgctcggt cgggatattg cgcgcctgcc gctggcggtg
    12901 gtctggctgt tcacttggcc tggtgtaccg tgcatttatt acggtgatga agtaggactg
    12961 gatggcaaaa acgatccgtt ttgccgtaaa ccgttcccct ggcaggtgga aaagcaggat
    13021 acggcgttat tcgcgctgta ccagcgaatg attgcgctgc gtaagaaaag tcaggcgcta
    13081 cgtcatggcg gctgtcgggt gctgtatgcg gaagataacg tggtggtatt tgtccgcgtg
    13141 ctgaatcagc agcgtgtact ggtggcaatc aaccgtggcg aagcttgtga agtcgtgcta
    13201 cccgcgtcac cgttgctcaa tgccgtgcaa tggcaatgca aagaagggca tgggcaactg
    13261 actgacggga ttctggcttt gcctgccatt tcggctacgg tatggatgaa ctaacgtttt
    13321 ataacgcctt gtgtgacgcc tgcgccatca tccgcggata aaactgccag aagcgggttt
    13381 cgagggcgtc ataatgagcg tctaaatcgt accaggagtc acgcagggca tccagacgtg
    13441 ggcggcggct tgccatgccg tttaacacgt tctggatgaa atccatatcg cgatagcgca
    13501 ccagccactg ctctgaccac aagtaattgt tcagattgat aaaacgtggc ggtgagtccg
    13561 gcaaaatcgt catcacttgc tcgcgggcat aacagacaaa ttcctgtagc ggaaagtccg
    13621 gcgacagctg cgaccagtgg cgggaaagaa agtgatccca catgacatcc agcgtaatag
    13681 gcgcaacgcg gcgagtttca ctacgaaacc attcccgtgc ttcgcggact tccggcagat
    13741 tgtcggttaa tacgtcgata cgtcgatgca tatgaatgcc agccacgacg tcgggcggaa
    13801 aactttcttc aggatttccg cgtacgaaat cagccagtaa attgccggaa agcgagcttt
    13861 ccgcgagatg ggctaaatgc aggtgagcta aaaaattcat cgattatatt ctatccaaaa
    13921 gggggtaaag gttgcaggga gagcgccccg gcactagact acccgcctct tattttagtc
    13981 tgagtcagtg tcatgcgcgt taccgatttc tcctttgaat tgcccgaatc cctgattgcc
    14041 cactatccca tgcctgaacg cagtagctgt cgtttactgt cgctggacgg gccgacgggc
    14101 gcgctgacgc acggtacttt caccgattta cttgataagc tcaaccccgg cgatcttctg
    14161 gtttttaata atacccgcgt gatcccggcg cgcctgtttg ggcgtaaagc cagcggcggc
    14221 aagattgaag tgctggttga gcggatgctc gacgacaaac gcattcttgc gcatattcgc
    14281 gcctcgaaag cgccaaaacc tggcgcagaa ctgctgctgg gcgatgacga aagtattaac
    14341 gcaacaatga ccgcgcgcca cggcgcactg tttgaagtcg aattcaatga tgatcgttcg
    14401 gtactggata tcctcaacag tatcggccat atgccgctgc cgccgtatat cgaccgtccg
    14461 gacgaagacg ctgaccgcga actttatcaa accgtttata gcgaaaaacc gggcgcggtt
    14521 gcagccccga ccgctggtct gcattttgac gagcctttgc tggaaaaatt gcgcgccaaa
    14581 ggcgtggaga tggcgtttgt gacgttgcac gttggtgcgg gcaccttcca gccggtgcgc
    14641 gtcgacacca ttgaagatca catcatgcac tcggaatacg ctgaagtacc gcaggatgtg
    14701 gtagacgcgg tactggcggc gaaagcgcgc ggtaaccggg tgattgcggt tggcaccact
    14761 tcagtacgtt cgctggaaag cgcggctcag gcagcgaaaa acgatctcat tgaaccgttc
    14821 ttcgacgata cccaaatctt tatctatccg ggcttccagt acaaagtggt cgatgcgctg
    14881 gtgacgaact tccacttgcc agagtcgacg ctgattatgc tggtttcggc ctttgccggt
    14941 tatcaacaca ccatgaacgc ctataaagca gcggtagaag agaaatatcg cttttttagt
    15001 tacggtgatg cgatgtttat cacgtacaat ccgcaggcaa ttaatgagcg cgtcggggag
    15061 taattccgcg gcgctggttt aaaacgttgg actgtttttc tgacgtagtg gagaaaaaat
    15121 gaaatttgaa ctggacacca ccgacggtcg cgcacgccgt ggccgcctgg tctttgatcg
    15181 tggcgtagtg gaaacgcctt gttttatgcc tgttggcacc tacggcaccg taaaagggat
    15241 gacgccggaa gaagttgaag ccactggcgc gcaaattatc ctcggcaaca ccttccacct
    15301 gtggctgcgc ccgggccagg aaatcatgaa actgcacggc gatctgcacg attttatgca
    15361 gtggaagggg ccgatcctca ccgactccgg cggtttccag gtcttcagcc ttggcgatat
    15421 tcgtaaaatc accgaacagg gcgtgcactt ccgtaacccg atcaacggcg atccgatttt
    15481 cctcgatcct gaaaaatcaa tggagattca gtacgatctt ggttcggata tcgtcatgat
    15541 ctttgatgag tgtacgccgt atcctgctga ctgggattac gcaaaacgct ccatggagat
    15601 gtctctgcgt tgggcgaagc gtagccgtga gcgttttgac agtctcggaa acaaaaatgc
    15661 gctgtttggt atcattcagg gcagcgttta cgaagattta cgtgatattt ctgttaaagg
    15721 tctggtagat atcggttttg atggctacgc tgtcggcggt ctggctgtgg gtgagccgaa
    15781 agcagatatg caccgcattc tggagcatgt atgcccgcaa attccggcag acaaaccgcg
    15841 ttacctgatg ggcgttggta aaccagaaga cctggttgaa ggcgtacgtc gtggtatcga
    15901 tatgtttgac tgcgtaatgc caacccgcaa cgcccgaaat ggtcatttgt tcgtgaccga
    15961 tggcgtggtg aaaatccgca atgcgaagta taagagcgat actggcccac tcgatcctga
    16021 gtgtgattgc tacacctgtc gcaattattc acgcgcttac ttgcatcatc ttgaccgttg
    16081 caacgaaata ttaggcgcgc gactcaacac cattcataac cttcgttact accagcgttt
    16141 gatggcgggt ttacgcaagg ctattgaaga gggtaaatta gagagcttcg taactgattt
    16201 ttaccagcgt caggggcgag aagtaccacc tttgaacgtt gattaatatt aataatgagg
    16261 gaaatttaat gagctttttt atttctgatg cggtagcggc aacgggtgca ccggcgcaag
    16321 gtagcccgat gtctttgatt ttgatgctgg tggtattcgg tctgattttc tatttcatga
    16381 tcctgcgtcc acagcagaag cgcaccaaag aacacaaaaa gctgatggac tccattgcca
    16441 aaggtgatga agttctgacg aacggtggcc tggttggtcg cgtaaccaaa gtagcggaaa
    16501 acggctacat tgctatcgcg ctgaatgaca ccactgaagt agttattaaa cgtgacttcg
    16561 tagctgccgt cctgccgaaa ggcaccatga aggcgctgta attaaaattt ttccctaagg
    16621 gaattgccgt gttaaaccgt tatcctttgt ggaagtacgt catgctgatc gtggtgattg
    16681 tcatcggtct gctgtatgcg cttcccaacc tgtttggtga ggatccggct gttcagatca
    16741 ctggtgcgcg cggagtcgcc gccagtgagc aaacgctgat ccaggtccag aaaacgttac
    16801 aagaagaaaa aataactgct aagtctgtgg cactggaaga gggcgctatt cttgcgcgct
    16861 tcgactccac tgacacccag ctgcgcgctc gtgaagcatt aatgggcgtt atgggtgaca
    16921 aatacgtcgt ggcgcttaac cttgccccgg caacgccgcg ctggctggca gctattcacg
    16981 ctgagccgat gaagctcggc cttgacctgc gtggcggcgt tcacttcctg atggaagtgg
    17041 atatggacac cgcgcttggc aaactccagg aacaaaatat cgatagcctg cgcagcgatc
    17101 tgcgtgaaaa gggcatcccg tataccaccg ttcgtaaaga aaacaactac ggcctgagca
    17161 ttactttccg cgatgctaaa gctcgtgatg aagccattgc gtatctgagc aagcgccatc
    17221 cggacctggt gattagtagc cagggcagca accagctgcg tgcggtaatg agcgatgctc
    17281 gtctgagtga agcgcgtgaa tatgcggtgc agcagaacat caatatcctg cgtaaccgtg
    17341 taaaccaact tggcgtggcg gagccggtgg ttcagcgtca gggtgctgac cgtatcgttg
    17401 ttgaactgcc aggtattcag gacactgcgc gtgcgaaaga gattctgggt gcgacggcaa
    17461 cgctggaatt ccgtctggta aacaccaacg ttgaccaggc cgctgcggca tccggtcgcg
    17521 taccgggcga ctctgaagtg aaacagaccc gcgaaggtca gccagttgtg ctgtacaaac
    17581 gcgtaattct gaccggtgac catatcaccg actccacttc cagccaggac gaatacaacc
    17641 aaccgcaggt taacatctcg ctcgatagcg ctggtggtaa catcatgtct aacttcacta
    17701 aggacaacat cggcaaaccg atggcaaccc tgtttgtgga gtacaaagac agcggtaaga
    17761 aagatgcaaa tggtcgtgcg gttctggtga aacaggaaga ggtgattaac atcgccaaca
    17821 tccagtctcg tctgggtaac agcttccgta tcaccggcat caacaacccg aacgaagccc
    17881 gtcagctgtc actgctgctg cgtgccggtg cgttgatcgc gccgattcag attgttgaag
    17941 aacgtaccat tggcccaacc ctgggtatgc agaacattga acaggggctg gaggcttgcc
    18001 ttgccggtct gctggtttct attctgttca tgatcatctt ctataagaag tttggtctga
    18061 ttgcgaccag tgctctgatt gccaacttga tcttaatcgt cggcattatg tcgctgttgc
    18121 caggcgcaac gctgagtatg ccaggtatcg cgggtatcgt cttaaccctt gcggtggcgg
    18181 tcgatgcgaa cgtactgatc aacgaacgta ttaaagaaga gttgagcaac ggacgtactg
    18241 ttcaacaggc aattgatgaa ggttatcgtg gcgcattcag ttctatcttc gatgcgaaca
    18301 tcaccacgct gattaaagtc atcatcctgt acgcagtggg taccggggca attaaagggt
    18361 tcgcgattac taccggtatc ggtgtggcga cgtcgatgtt taccgcgatt gtcggtacgc
    18421 gtgccatcgt aaacctgcta tatggcggca agcgcgtcaa gaagctgtca atctgaggag
    18481 tgcgatgtgg cacaggaata tactgttgaa caactaaacc acggccgtaa agtctatgac
    18541 tttatgcgct gggactactg ggctttcggc atctctggtc tgctgttaat cgccgctatc
    18601 gttattatgg gcgtgcgcgg ctttaactgg gggctggatt tcaccggtgg tacggttatc
    18661 gaaattacgc tcgaaaaacc ggctgaaatt gacgtaatgc gtgatgcatt gcaaaaagcc
    18721 ggttttgaag agccaatgct gcaaaacttt ggtagcagcc acgacatcat ggtccgtatg
    18781 ccgcctgctg aaggcgaaac cggcggtcag gtgctgggca gccaggttct gaaggtgatt
    18841 aacgaatcca ccaatcagaa tgcagcagtg aagcgtattg agttcgtcgg tccgagcgtg
    18901 ggggcagacc ttgcgcaaac cggtgcgatg gcgttgatgg cggcgctgct gtctatcctt
    18961 gtgtacgtag gttttcgctt tgagtggcga ctggcggcag gggtcgttat tgcgctggcg
    19021 cacgacgtta tcattacgct gggtattttg tcgttattcc atatcgagat tgacctgacc
    19081 attgtggcat cgttgatgtc ggttatcggt tactcgctta acgacagtat cgtggtatcg
    19141 gaccgtattc gtgaaaactt ccgcaagatc cgtcgcggta cgccttacga aatctttaac
    19201 gtgtccttga cccagacgct gcaccgtacc ttgatcacat ccggtactac cttgatggtt
    19261 atcctgatgc tgtacctctt cggtggtccg gtactggaag gcttctcgct gaccatgctt
    19321 atcggtgttt ccatcggtac tgcatcttcc atctatgtgg catctgcgtt ggctctgaaa
    19381 ctgggtatga agcgcgaaca catgttgcag cagaaagtgg aaaaagaagg ggcggatcag
    19441 ccgtcaattc tgccgtaatc aagttcccgt tgatgttgaa aatcccggtc agaagatcgg
    19501 gatttttttt gatgtatgga gattgctaaa atccccgcat cttgggaaac tgcgcgtaac
    19561 cctacatttc atccaggtaa ctcttcatgg ctatcatccc aaaaaactat gcgcggttag
    19621 aaagcggcta tcgcgaaaaa gcattaaaaa tatatccgtg ggtctgcggt cgctgttccc
    19681 gcgagtttgt ttattccaac ctgcgtgaac ttactgttca ccacattgat cacgaccata
    19741 ccaataaccc ggaagatggc agtaactggg aattgctttg tctctattgc cacgatcatg
    19801 agcattcgaa atataccgaa gcggatcagt atggtacgac cgttatcgca ggggaagatg
    19861 cgcagaaaga tgtcggtgaa gcgaagtaca acccattcgc tgacctgaaa gcgatgatga
    19921 acaagaagaa gtgattaaaa cgtaaaattg cctgatgcgc tacgcttatc aggactacgt
    19981 tatttcagca atatattgaa tttacgtgct tttgtgggtc ggacaaagcg tttacgccgc
    20041 atccggcatg aacaaagcgc acgttgttaa caatctgaaa tgccgggaat aaatcccggc
    20101 attttcataa tcagaagttg taacctacaa ccaggtaacc accccagccg gtagagcgaa
    20161 cgttgaagtt gccgttgccg aagttcagtt ctgcatcgtc gttccactga ccaccgtcgt
    20221 gccagtaacg agctacgaca gagtagtgcc agtgatcgta gttcagagcc agaatatggc
    20281 tggaagcgat agagttatta gtacgggtct taataccgtt gattgcgtta ccgctgtcat
    20341 cccctaaatc ggaaccccag tcgaagttgg tgaagccgat gtagctcagc tgaccgcccc
    20401 acagatcggt aatcggcaca aagtatttaa ttttgaaacg gtaaccgtcc cactcgtttt
    20461 cgttcgctgc gccatagttc tgccactggt atttcgcata gacgttcatg gacaggctca
    20521 tcggcagacc agtatcgatg tcggtaccca gacccatata ccaggtgctc tggcgaccat
    20581 ctttattacg acccatgtcg taaatgtagt tgttcgcgaa gtaccactct ttgaacggac
    20641 cgaagctaag gtcagtattg gtcagcttgt cgatggagaa acgtggttcg atttccataa
    20701 acagcggaga gccgtggttc cagatacctt tggcatcgga gttaccgccg aagaataccg
    20761 gcgcatccgc ataaccatag aagtcgaacc agtctttttt agcgaatgct tcgtactcaa
    20821 ggtaggtatc gttgcggatc tgcggtccaa aacgggtgtg atagcttccg acaacgttaa
    20881 cgctctggtg ccaccagtcg gaaagatact gcggtttgtc gttttcagct gcgttgacag
    20941 taaaagacga agagagcgcc agtaccgcac cggctgccag taatgttttt ttcatatgta
    21001 tgccactgtt tgaaaatccc ttgcgggagt gaaaaaggcg caaattgcgt ttctaaatat
    21061 ttcgtataca gaggagccta ttataaaaat cattgctcac aaaaatatgt ttcgtttcac
    21121 agttctatca tttacgtaat cgattgcgtt cacgtttaca cacattcggg cagggattgt
    21181 actgactttc acccttgttg caaactttta ctatcaatca gggtgatgta agcggagtaa
    21241 caaaatgacg gggaaagatg ttgcgggacg cacaaaatcg ccccgaaata cgtttactgc
    21301 ataacaggtt gaatatcgtg gatacgtacg aagtctaact gttctacaga aatacccttt
    21361 aactgtaacg gaatatcaac atcgctgggg gcgaggacgc tggcaggggc attaatcaat
    21421 tgattctgca cattgatttc ctgaaagttg tctattgttc cctgaatttg cccatattca
    21481 acagttccgc tgaatgcggg caagggatca ttcgactcgc cctgaatacg tagcgtcagg
    21541 gtggtgccat ctgcatcagg cgtaatattc accagcgaca tacgtaaagt accgatctgg
    21601 ctttccagtc ttgccggtgt tttcgctcca ggcagaaggt aaaccccact gctggatttt
    21661 gcattcagcc tgttttgctg agtgattttg accgtttctt tattgagttg cgtcatctcc
    21721 tggttcagcg tgctgacgct gtgtttcatc tgtcgcactt cgctctgctg cacacaggcg
    21781 ctaaggctga acaggcttcc cagcaggagc agtcgaaaaa cgttagtatt cataggtcgt
    21841 ccctttctcg tcgcaattag cctaatggta gagggtaaaa gcagcgaaag catcgatcct
    21901 ttatctcaaa agcgttgcgc ctttgttgta tcgtcagttc agggtaaaat agatttctgt
    21961 taaccacctg gtcaggacgc cgtatgcatt gcccattctg tttcgccgtg gacactaagg
    22021 taattgactc tcgtctcgtg ggcgagggtt catccgtacg ccgccgtcgg cagtgtctgg
    22081 tgtgtaatga acgtttcacc acctttgaag tggcggagct ggttatgccg cgtgttgtaa
    22141 aaagcaacga cgtgcgtgaa ccgtttaatg aagagaaatt gcgtagcgga atgctgcggg
    22201 cgctggaaaa acgtccggtg agttccgatg acgtcgaaat ggcaatcaat catattaaat
    22261 cgcagctgcg cgccaccggt gagcgcgaag taccgagcaa gatgattggc aatctggtga
    22321 tggagcaatt gaaaaagctc gataaagtcg cctatatccg ttttgcctct gtctaccgca
    22381 gtttcgaaga tatcaaagaa tttggcgaag agatcgcgcg cctggaggac taagccgtgc
    22441 aggacgagta ttacatggcg cgggcgctaa agctggcgca acgaggacgt tttaccacgc
    22501 atcccaaccc gaatgtcggg tgcgtcattg tcaaagatgg cgaaattgtc ggtgaaggtt
    22561 accaccaacg tgcgggtgaa ccacatgccg aagtacacgc gttgcgtatg gcgggtgaaa
    22621 aagccaaagg tgcgaccgcc tatgtcacac tcgaaccctg tagccatcat ggtcgtacgc
    22681 caccgtgctg tgacgcactc atcgccgctg gcgtagcgcg cgtggttgcc tcgatgcaag
    22741 atcctaaccc gcaggtcgct gggcgtggac tttaccgtct gcaacaggct ggcattgacg
    22801 tcagccacgg gctgatgatg agtgaagccg agcaattgaa taaaggcttt ctcaagcgga
    22861 tgcgcaccgg ctttccttat attcagttaa aacttggcgc atcgcttgat ggccgcacgg
    22921 cgatggcgag cggcgaaagc cagtggatca cttcgcctca ggcgcggcgc gacgtacaac
    22981 gacagcgcgc acaaagtcat gccattttaa ccagcagcgc cacggtgctg gcggatgatc
    23041 cagccttaac agtgcgttgg tctgaactgg atgaacaaac tcaggcgctc tacccgcaac
    23101 aaaatctccg ccagccagta cgtattgtga ttgatagcca aaatcgcgtg acgccggaac
    23161 atcgcattgt gcagcagccc ggcgaaacct ggttcgcgcg tacgcaggaa gattctagtg
    23221 agtggccgga aacggtgcgt accttgctga ttccagagca taaaggtcat ctggatctgg
    23281 ttgtactgat gatgcaactg ggtaaacagc aaattaacag catctgggtg gaagcggggc
    23341 caacgctcgc tggcgcattg ctgcaggcgg gtttagtcga tgagctgatt gtctatatcg
    23401 cacctaaact attaggcagc gacgcccgcg gattatgcac gctgccaggg cttgagaaat
    23461 tagccgacgc cccccaattt aaattcaaag agatacgtca tgtaggcccg gatgtttgcc
    23521 tgcatttagt gggtgcatga tctcccggct cgaaagggga gcagcgcacg aaatattatg
    23581 ctaaaatccg cccccctgcg gggccatact cgaacccgaa ggaagaaaat gaacattatt
    23641 gaagctaacg ttgctacccc ggacgctcgc gtcgccatca ccatcgcgcg tttcaacaac
    23701 tttatcaatg acagcctgct ggaaggtgca attgacgcac tgaaacgtat cggtcaggta
    23761 aaagatgaaa acattaccgt tgtttgggtg cctggtgcct atgagctgcc gctggcggcg
    23821 ggtgcactgg ctaaaaccgg taaatacgac gcggtgattg cgctgggtac ggttattcgt
    23881 ggtggcactg cccactttga atatgtcgct ggtggtgcaa gcaacggcct ggcgcatgtt
    23941 gcccaggaca gcgaaattcc ggttgctttt ggggttctga ccactgaaag cattgaacaa
    24001 gcgatcgaac gtgctggcac caaagctggc aacaaaggtg cagaagctgc actgaccgcg
    24061 cttgaaatga ttaatgtatt gaaagccatc aaggcctgaa attagtaagg ggaaatccgt
    24121 gaaacctgct gctcgtcgcc gcgctcgtga gtgtgccgtc caggcgctct actcctggca
    24181 gttgtcccag aacgacatcg ctgatgttga ataccagttc ctggctgaac aggatgtaaa
    24241 agacgttgac gtcctgtact tccgtgagct gctggccggg gtggcgacta acaccgcata
    24301 cctcgacgga ctgatgaagc catacctgtc ccgcctgctg gaagaactgg gacaggtaga
    24361 aaaagcagta ctgcgcattg cgctgtacga gctgtctaaa cgtagcgatg tgccatacaa
    24421 agtggccatt aacgaagcga tcgaactggc gaaatcgttc ggcgcagaag acagccataa
    24481 gttcgtcaac ggcgtactcg ataaagcagc acctgtgatt cgccctaaca aaaagtgata
    24541 tccaggccgg tagattcacg gaagaccgtt ccgcgatcgc cggccttttc ttttttacct
    24601 gctgaggcat aacgtatggc atgtggcgag ttctccctga ttgcccgtta ttttgaccgt
    24661 gtaagaagtt ctcgtcttga tgtcgaactg ggcatcggcg acgattgcgc tcttctcaat
    24721 atccccgaga aacagaccct ggcgatcagc actgatacgc tggtggcggg caaccacttc
    24781 ctccatgata tcgatcctgc tgatctggcg tataaagcac tggcggtgaa cctaagcgat
    24841 ctggcagcga tgggggccga tccggcctgg ctgacgctgg cattaacctt accggacgta
    24901 gacgaagcgt ggcttgagtc cttcagcgac agtttgtttg atcttctcaa ttattacgat
    24961 atgcaactca ttggcggcga taccacgcgt gggccattat caatgacgtt gggtatccac
    25021 ggctttgttc cgatgggacg agccttaacg cgctctgggg cgaaaccggg tgactggatc
    25081 tatgtgaccg gtacaccggg cgatagcgcc gccgggctgg cgattttgca aaaccgtttg
    25141 caggttgccg atgctaaaga tgctgactac ttgatcaaac gtcatctccg tccatcgccg
    25201 cgtattttac aggggcaggc actgcgcgat ctggcaaatt cagctatcga tctctctgac
    25261 ggtctgattt ccgatctcgg gcatatcgtg aaagccagcg actgcggcgc acgtattgac
    25321 ctggcattgc tgccgttttc tgatgcgctt tctcgccatg ttgaaccgga acaggcgctg
    25381 cgctgggcgc tctctggcgg tgaagattac gagttgtgtt tcacggtgcc ggaactgaac
    25441 cgtggagcgc tggatgtggc tctcggacac ctgggcgtac cgtttacctg tatcgggcaa
    25501 atgaccgccg atatcgaagg gctttgtttt attcgtgacg gcgaacctgt tacgttagac
    25561 tggaaaggat atgaccattt tgccacgcca taaagatgtc gcgaaaagtc gcctgaagat
    25621 gagtaatccg tggcatctac ttgctgtcgg attcggaagt ggattaagcc cgatcgttcc
    25681 tgggacgatg ggctcgctgg cagcgattcc gttctggtat ctgatgacct ttttgccctg
    25741 gcagctctac tcgctggtgg tgatgctggg gatctgtatc ggcgtctatc tttgtcatca
    25801 aacggcgaaa gatatgggcg tgcacgatca cggcagcatt gtctgggacg aatttattgg
    25861 tatgtggatc acgctcatgg cgctgccgac caatgactgg cagtgggttg ccgccgggtt
    25921 tgtgattttc cgtattctgg atatgtggaa accgtggccg atccgctggt ttgatcgcaa
    25981 tgtgcatggc ggcatgggga tcatgataga tgatattgtc gccggggtga tctccgctgg
    26041 catcctgtac tttattggtc atcactggcc gctgggcatt ctgtcgtagt tgtgcaccga
    26101 tgcctgatgt gacgcttgtt gcgtctcatc aggcctggac tcttatttaa atcctactac
    26161 cggatgcggt ttatacggcg tttccagttc ggcaatctgt tccggcttca aagtgatatc
    26221 caccgcgttc agcagctcat caagctgttc ttcccgcgat gtaccgataa tcggtgcggc
    26281 aatgcccggt ttactcaaca accaggccag cgcaacttgt gctcgtgttg caccgagttc
    26341 ttcactgacg cccgttaacc gctctgcgat ctgcgcgtca ttttcatcgc tttctttata
    26401 gagatttttc cccacctcat cagacaccag tcgtgcggta gtttctcccc acggacgcgt
    26461 cagacggccc ctcgccaacg ggctccatgg aatcaccgcc acgccctcct gataacacag
    26521 aggcagcatc tcgcgctctt cttcgcgata aatcagattg tagtgatcct gcatactgac
    26581 aaactgcgcc cagccgtgct gtttttggag ttccagtgcc tgagcaaact gcgaagcgtg
    26641 cattgatgac gcgccgatat aacgcgcttt cccggctttt accacgtcgt tgagggcttc
    26701 cagcgtctct tcgatcggcg tgttgtaatc ccagcgatga atttgcagga tatcgacata
    26761 atccatgccg agacggcgca ggctgtcgtc gattgagcgc aaaatttgtg cacgggataa
    26821 tccttccggt aaatcaccaa cgcgatggaa cactttggtg gcaacgacca cgtcttcacg
    26881 acgggcgaaa tcccgcagtg cgcgaccaac gatctcttcg ctgctgccat cggaatagct
    26941 attggcggta tcaaagaaat ttatgccgcc ttccagcgcg cgtttaatga tgggacggct
    27001 actctcttcc ggcagtgtcc atgcgtgatt accacgatct ggctcgccaa aggtcataca
    27061 gccgaggcaa agtcgggaaa cgcgaaggtc ggtttttcct aaggggttgt attgcatgct
    27121 gccactcctg ccatactcgt catacttcaa gttgcatgcg ctgcggctgc attcgttcac
    27181 cccagtcact tacttatgta agctcctggg gcttcactcg tttgccgcct tcctgcaact
    27241 cgaattattt agagtctatg aataatttgt taagcatagc aggagtggag tagggattat
    27301 gccagccagg ccttgatttt ggcttccata ccggcggcat cgaggccgag ttcggcgcgc
    27361 atttcttcct gagttccttg cggaataaag aagtccggca gaccaatgtt cagcacgggt
    27421 actggtttac gatgggccat cagcacttcg ttcactccgc tacctgcgcc gcccataatg
    27481 gcgttttctt ctacggtgac cagcgcttca tggctggcgg ccatttccag aattaacgct
    27541 tcatcaagcg gtttcacaaa acgcatatcg accagcgtgg cgttcagcga ttcagcgact
    27601 ttcgccgcgt ctggcatcag cgtaccaaag ttaaggatcg ccagtttctc gccacgacgc
    27661 ttcacaatgc ctttgccaat tggcagtttt tccagcggcg tcagctccac gcctaccgcg
    27721 ttgccacgcg ggtagcgcac cgctgacggg ccatcgttat agtgatagcc ggtatagagc
    27781 atctggcgac attcgttttc atcgctcggg gtcataatga ccatttccgg tatgcagcgc
    27841 aggtaagaga gatcaaaagc accctgatgg gtttgaccgt cagcaccaac aatgcccgcg
    27901 cggtcgatgg cgaacaggac cgggagtttt tgaattgcca cgtcatgcag cacctgatca
    27961 taggcgcgtt gcaggaaggt ggagtagatc gcgacaatgg gtttgtatcc accaatggcc
    28021 agtcccgcag caaaggtcac tgcgtgttgc tcggcaatcg ctacatcaaa atagcgatcc
    28081 gggaatttac gagaaaactc gaccataccg gaaccttcac gcatcgccgg agtaatcgcc
    28141 atcagcttgt tgtctttcgc tgccgtttcg cacaaccagt cgccaaagat ttttgaatag
    28201 ctcggcaaac cgccgctact tttcggcaaa caaccgctgg agggatcaaa tttaggcacg
    28261 gcgtggaaag tgattgggtc tttttctgcc ggttcataac cacgaccttt tttggtcatg
    28321 atatgcagga actgcgggcc tttcaggtca cgcatgttct tcagcgtggt gataagcccc
    28381 agcacatcgt gaccgtcaac cgggccgatg tagttaaagc ccagctcttc aaacaacgtg
    28441 ccagggacta ccatgccttt aatatgttct tcggtacgtt tgagtagctc tttaatgggt
    28501 ggcacgccag agaaaacttt tttcccgcct tcgcgcagtg aagagtaaag cttaccggaa
    28561 agcagctgcg ccagatggtt gttgagcgcg ccgacatttt cggaaatcga catttcgttg
    28621 tcgttgagga ccaccagcat atcaggacgg atatcgcccg cgtgattcat cgcttcaaac
    28681 gccatgcctg cggtaatcgc gccatcgccg attacacaga cggtacggcg atttttgtct
    28741 tctttctcgg cagcaaccgc aataccaatt ccggcactga tggaggttga tgaatgcccg
    28801 acgcttaata cgtcatattc gctttcgccg cgccacggga acgggtgcag gccgcctttc
    28861 tgacggatgg tgtcgatttt gtcgcggcgt ccggtcaaaa ttttatgcgg ataagcctga
    28921 tgccccacat cccaaatcaa ttggtcaaac ggggtgttgt agacatagtg cagcgccacg
    28981 gtcagttcga ccgtgcccag cccggaggcg aagtgcccgc tggaacggct cacgctgtcg
    29041 agtaaatagc ggcgcagttc gtcgcagagt ttcggtaaac tctctttcgg caacagtcgt
    29101 aactcctggg tggagtcgac cagtgccagg gtcgggtatt tggcaatatc aaaactcatc
    29161 aggggcctat taatacttat tgtttattta ttacgctgga tgatgtagtc cgctagcgct
    29221 tccagtgccg aggtatcgag tgattgttca gccagttgtt tcagcgactg gcgggcatcg
    29281 tcgatcagat cccgggcttt cttccgggct tgctcaagac ccagaagtgc agggtaggta
    29341 cttttaccaa gttgctggtc ggcaccctgg cgttttccca acgttgcagt atctcctacc
    29401 acatccagga tgtcatcctg aacctggaag gcaaggccga tgctctctgc gtacttgtcg
    29461 agtactggca gagcacgacg ccctttatct ccggcgctta atgcaccaag gcgaacggcg
    29521 gcgcgaatca atgcgccggt tttatgacga tgaatacgct caagcgcgtc cagaggtacg
    29581 tgtttgcctt ccgcgtctaa atctaatgcc tgaccaccgc acattccggc aataccgctg
    29641 gcgctcgcca gttcagaaat catcgaaatt ctgtcgcgat ccgacacttc cggcatattg
    29701 gcatcgctta gaatcgagaa cgccagcgtt tgtaaagcgt cgccagcgag aatcgcgttt
    29761 gcttcgccaa acttcacatg gcaggtcggc aaaccgcgac gcagatcgtc atcatccatc
    29821 gccggtaaat catcatgaat taatgagtaa gcgtggatac actctacggc ggcagcgggt
    29881 gcgtccagcg tgtttgtgct aacgccaaac atatgaccgg tggcataaac caggaagggt
    29941 cgcagacgct taccacctaa taatgcgcca tactgcatgg tttcgaccac gggagtgttc
    30001 tgaaagggca gtggggcgat aaaacggctc agcgcctggt tggcctgctt aacgcaggct
    30061 tcgagttgct gcggaaagtc cattactcat tgtccggtgt aaaaggggtt agagaggcgt
    30121 cttcattgtc agacagcaga atttgtacgc gctgttcggc ttgttgtaat ttggcctgcc
    30181 cctgacgtgc cagctgcacg ccgcgttcga actcgttcag cgcctcttcc agcggcaggt
    30241 cgccactttc cagacgggtt acaatctgtt ccagctcgct cagcgccttt tcaaagctgg
    30301 cgggagcctc atttttcttc ggcataatga atgtctgact ctcaatattt ttcgccccgt
    30361 catggtaacg gactcagggc aaatagcaaa taacgcgcaa tggtaaggtg atgtgcgcag
    30421 caaagcgatg ttagtggtat acttccgcgc ctggatgcag ccgcaggtgt gggctgctgt
    30481 atttttccct atacaagtcg cttaaggctt gccaacgaac cattgccgcc atgaagttta
    30541 tcattaaatt gttcccggaa atcaccatca aaagccaatc tgtgcgcttg cgctttataa
    30601 aaatccttac cgggaacatt cgtaacgttt taaagcacta tgatgagacg ctcgctgtcg
    30661 tccgccactg ggataacatc gaagttcgcg caaaagatga aaaccagcgt ctggctattc
    30721 gcgacgccct gacccgtatt ccgggtatcc accatattct cgaagtcgaa gacgtgccgt
    30781 ttaccgacat gcacgatatt tttgagaaag cgttggttca gtatcgcgat cagctggaag
    30841 gcaaaacctt ctgcgtacgc gtgaagcgcc gtggcaaaca tgattttagc tcgattgatg
    30901 tggaacgtta cgtcggcggc ggtttaaatc agcatattga gtccgcgcgc gtgaagctga
    30961 ccaatccgga agtgactgtc catctggaag tggaagacga tcgtctcctg ctgattaaag
    31021 gccgctacga aggtattggc ggtttcccga tcggcaccca ggaagatgtg ctgtcgctca
    31081 tttccggtgg tttcgactcc ggtgtttcca gttatatgtt gatgcgtcgc ggctgccgcg
    31141 tgcattactg tttctttaac cttggcggcg cggcgcatga aattggcgtg cgtcaggtgg
    31201 cgcattatct gtggaaccgt tttggcagct cccaccgcgt gcgttttgtc gctattaatt
    31261 tcgaaccggt cgtcggggaa attctcgaga aaatcgacga cggtcagatg ggcgttatcc
    31321 tcaaacgtat gatggtgcgt gccgcgtcta aagtggctga acgttacggc gtacaggcgc
    31381 tggtcaccgg cgaagcgctc ggccaggtgt ccagtcagac gctgaccaac ctgcgcctga
    31441 ttgataacgt ctccgatacg ctgatcctgc gtccgctgat ttcttacgac aaagagcaca
    31501 tcatcaacct ggctcgccag attggcaccg aagactttgc tcgcacgatg ccggaatatt
    31561 gcggtgttat ctccaaaagc ccgacggtga aagcggttaa gtcgaagatt gaagcggaag
    31621 aagagaaatt cgacttcagc attctcgata aagtggttga agaagcgaat aacgttgata
    31681 tccgcgaaat cgcccagcag accgagcagg aagtggtgga agtggaaacc gtcaatggct
    31741 tcggcccgaa cgacgtgatc ctcgatatcc gttctatcga tgaacaggaa gataagccac
    31801 tgaaagtcga agggattgat gtagtttctc tgccgttcta taaactgagc accaagtttg
    31861 gcgatctcga ccagaacaga acttggctac tgtggtgtga gcgcggggtg atgagccgtc
    31921 tgcaggcgct ctatctgcgc gagcagggct ttaacaatgt gaaggtatat cgcccgtaat
    31981 ttgtggttgt aacgtcgcat ctggtcagat gcgacgctta ccgcatccga cactactcgt
    32041 aataattata aatccctgcc gccatcacca gttgtgatgc cacttcatgg gctttctcgc
    32101 gcccaaccag caggtcgata attttcagac caaagtcgat agctgtcccc ggcccctggc
    32161 tggtcagcaa ttttacccgt gcatcccaga cgacgcgctt gtcctgccat tgttcagcgg
    32221 gaattttgtc tttcagcgtc gggaagccgg tcatattacc aatcgggaag atatcgtgcg
    32281 gcaccagcac gatggctggc gcggcgcaaa tagccgcgac gatacgcccg gaacggtgga
    32341 actgtttaac ggtttcaacc agcagggtac tatcgcgaaa acactctgcg cctttaatgc
    32401 caccaggcag tacgatcacg tcatattcgc catcagccac ctcgaccagc ggcgcatccg
    32461 ccagcagctt cacgccgcgc gagcaggtaa tggccaggtt gccatcgctg gcgacgctgg
    32521 cagtggtgac tttgataccg ccgcgaacca gcagatcgat agtggtgacg gcttcagtct
    32581 cttcactacc aggggcgagg caaaccagtg ccgatgcgct catattcact ctcctttctt
    32641 tttaccattt caaacaggcg ggtgttttcc ggtacggcaa tcccatgcgc gcgggcgcgg
    32701 cgtaagagaa aaccattgat atagtcgatt tcggtgtggc gcagcgcgcg gatatcctgc
    32761 aacatcgacg agatattttc cgctgtggca tcaatcacct gcatcacgta atcacgcaaa
    32821 tcttctgctg aagtatgatg cccttcgcgc tcgatcactg ccgcgacttc ttcgcatatc
    32881 tgcataattt cttgcggatg atgacgtaat tcaccattcg ggcaattcca gatggcggtc
    32941 agtggattaa tcacacagtt gacggccagc ttgcgccaca gctcggcgcg aatattgtta
    33001 tgccaggcaa cgtcaggcaa cacggtttgc aaaatatccg ccagataact gtaatcgcca
    33061 tcctgttgcc gtgccggacc gatatgcgtg attccgtttg ccacatgaat aatgacattg
    33121 ccgtcgcggc gggcggcatg ggtggtggtg cccatcagta atggctgctg aatgttttgc
    33181 aattcttcga tggtgcccat gccgttgtga attaacagta ttggcgtagc tacaggcagt
    33241 gtggatgcga ggcttttgac ggcatcggaa acctgccatg ctttcagcgt caccaggagc
    33301 agatcgctgg tggcgagaaa atcgggatcg ttggcggtca gcgattcgtt aaatatcgaa
    33361 ccatctgtct caaccagatt cacactacaa taaggttgcg gtacgcgcag ccagccctga
    33421 acttcatgac cctgtttaca aagtgctgta agccataatt gccctaaggc accgcatccc
    33481 aatacggtaa ttttcattgt tcctcctcac ccgcaaccac tccgggtgtt caataaggct
    33541 atcccttaat tgtgcatgct gttgcgacta tgcacaatta agggatacgt cctggtgcag
    33601 gactgttggt tatttaactt tgcaggtatt atgcttcgca tcaaaaatga agggagagga
    33661 aaagatgcca tctttcgata ttgtctctga agttgatctt caggaagcac gtaacgcggt
    33721 cgataacgcg agtcgcgaag tggagtcccg ttttgacttc cgtaacgttg aagcctcatt
    33781 tgagctgaac gacgccagca aaaccatcaa agtgttgagc gagtccgact tccaggtcaa
    33841 tcagttgctg gatattctgc gtgccaagct gctgaagcgc ggcattgaag gcagttcact
    33901 ggatgtaccg gaaaatatcg ttcatagcgg taaaacctgg tttgtggaag cgaaactgaa
    33961 acagggcatt gagagcgcga cccagaagaa aatcgtcaag atgatcaaag acagcaaact
    34021 gaaagtgcag gcgcaaattc agggcgatga aatccgcgta acgggcaaat ctcgtgatga
    34081 tttgcaggct gtcatggcga tggtacgtgg tggcgatctc ggccagccgt tccagttcaa
    34141 aaacttccgc gattaatcgc gacaatgtgc gttttgttca tgtcagatgc ggcatgaacg
    34201 cctgattcgg cctacaacgg tttgtaactc aagaactttg taggcctggt aagcgtagcg
    34261 cagcaggcaa ttcgccgcgt tttttatgcc tgacgaattg cctgttcaac ctcaaagcga
    34321 tttgtcactt tgctgtcgat tttcacataa gctgaatgct cttcttctgc aatcaacact
    34381 tctttgacgc cttcagtttc cagcaaacgc acttttaacg cttcgtttgc ggcaatgtcc
    34441 gccgggattt caatgcgcaa actgctgaca tacggcggtt ctttcatggt actggcgact
    34501 gccagccaca ctgcggccag cattgcgcca gcgagaaata ccccctgacc gtcaaacatg
    34561 ccgtcaatcc agccgcccag cgaaccgcca atcgccacgc caagaaactg gctggtggag
    34621 tagacaccca tcgccgtacc tttgtaacct gctggcgact ctttactgat aagtgagggc
    34681 agaagggctt ccatcaaatt aaacgccaca aagaaaagct gcacgccgac caccagttgc
    34741 cagaactgcg tttgtgcgtt ccacaacaca atttccgcaa ccacgatcaa cccgacgcag
    34801 aagacaaaga cttgcttcat tttgcgctta acttcagcgt agataatgaa aggcacgacc
    34861 gagccaaagg cgattaacat cgtcgccaga tagaccttcc agtgttcagc cgccgggaac
    34921 cccgcatcag ctagctgtcc gggcagggca acaaacgtag acatcagaag catgtgcaga
    34981 cacataatgc caaagttgag ttttagcagc cgcggttccg ccagcacttt actgaaactg
    35041 cctttcacca ttccggactc acgattaagt acgtgagtgc tactgttggg cacaacccaa
    35101 atggtcaacg caatgccggt tgttgccaga atagcgatca tccagaacag cgcgtgcagc
    35161 ccaagtttgt gagtgatgat cgggccaagc accatcgcaa tggcaaaggt aatgccaaag
    35221 ctcacgccga taaacgccat cgctttggtg cggttttgtt cgcgcgtgag atcggaaagc
    35281 agcgccataa cggcggcggc aattgctcca gagccttgta gcgcccggcc cagaataatt
    35341 ccccagatgg agtcggaaag cgcagcgata acgctaccgg cggcaaacac cgccagccca
    35401 ccgacaatta atggtttgcg accaatgcgg tcggaaagca ggccaaacgg aatctgaaaa
    35461 acggcctgag tcagaccata aataccaatg gcaataccga ttaatgcttc gctggcacct
    35521 tgcagagcca tgccgtacgt ggtcagaacc ggcagaacca taaacatgcc cagcatgcgc
    35581 aacgagaata cggtccctaa accccaggtc gcgcgcctct cacctggcgt cattttataa
    35641 tcgttcatta ccacctctgt tttaaattcg cgactagtgt aaagcggcaa acgaatgggg
    35701 taaacatgcg gattttttag taaatatttc gagagggata tgtttctaat gctaagaaaa
    35761 aaggtgccgt agcacctttt taactgagag gttgtgttac cacacagcag ccagcagcgt
    35821 atgcgagtcc ggtaccataa aatcaacgga catcatcacc gagagggcag tgatggcgat
    35881 gatagagaag ccgaacagct tgcgcgccca gattctgtca tcagcaactt tataaccgcg
    35941 cagagccata cctaaccacc agacgctaac cgccgcggcg accaccagat atttatatcc
    36001 agcgtaaccg ccaagagaga gcatcagcgt ggcaacggca aaggcgatga tatacagcgt
    36061 gatgtgattc ttcgccaccg aaatgccttt taccactggc aataccggaa tgtttgccgc
    36121 ctggtaatcc ttaaagcgga aaatggcgat ggcataggag tgaggcatct gccacaggct
    36181 gaagatagcc agcaggatcg ctgcgccgct atcgaactcg ccggttactg cacagtagcc
    36241 aatcaccggc ggcgcagcgc cggagagcga accaatcaat gtgccgtaga ctgagtggcg
    36301 tttcatgtac aggctataga cgccgacata aaccacaaag cccatcaccc ccagccagca
    36361 ggccagcgga ttcgcgccaa accacagcag cataaagcca gcaataccca gcaaggtggc
    36421 gtacaccagc gagacagcag gagagatcag gcctttaacc agcacccgat tcttcgtcct
    36481 ttccatcttt ctgtcgatat ccctgtcgat gtagttgtta aacacacaac ccgacgccac
    36541 aaccagtgac accccaacca gcgtgtagat aaacagggga taatcaatgc tgccctttga
    36601 ggccagcagg aatcccccaa tcaccgagat caggttgcca aagatgatgc ctggtttcgt
    36661 tacttgcagg tattgcttaa acatcataac cgccgctctt agtgcatcat catgttgtag
    36721 ttgaggttcc acataatcca gatggagcct acaaccagga tagcgatgat tagcacggtg
    36781 aagacaaacg ccgtcatgtt ccagccttca tctgatttgg tattcatgtg caggaagcac
    36841 accagatgca ccagaacctg taccactgcc attgccagga ttgttcccag aattacggcc
    36901 ggagaggcag cccctgtcat caccatccag aacggaatca ccgtcagaat gatcgacagg
    36961 ataaagcctg tcatgtaggt ttttacgctg ccatgggacg cgccgctgtg atcggtagaa
    37021 tgactcatta catcgccccc atcagataaa caacagtgaa cacacagatc caaaccacat
    37081 ccaggaagtg ccagaacagg ctcaggcaca tgatgcgggt acggttagtg ctggtcaggc
    37141 cgcgacgggc gatttgcacc atcagcaccg ccatccagat aagaccagaa gtgacgtgca
    37201 gaccgtgcgt gccgaccagc gcaaagaacg ctgacaggaa gccgctgcga tccggaccca
    37261 tgccgttaac aatcaggtga tggaattcat agatttccat cccgataaat ccggcaccaa
    37321 acaaccaggt caacgccagc caggagataa cctggctttt gttgtttttg tacatggcga
    37381 tagccgccat gccgtaggtg atggagctga acaacagcaa gaaagtttca accagaacga
    37441 acggcagttc gaaaatgtcc ttacctgtcg ggccgcctgc ggtgccgttc accagaacgg
    37501 cataggtagc aaacaagatt gagaacagaa tgcagtcgct catcaggtag atccaaaatc
    37561 cgaagatttt ggttccgcct gcatcgtggt gcccgtgttc gtgcgcgtgg gcagtcgcgt
    37621 gcgtcaaagt atcagttgcc atttttcagc cctgccttag taatctcatc gaaatgctgg
    37681 ttttccagtt tttcgatttc tgccaccggc acgtagtaat ccacgtcctc gtcgaagctt
    37741 ttcacgatcc aggtgatgat catgcctgcg aagccaacaa tcgccagcca ccagatatgc
    37801 cagatcatgg cgaaaccgaa gatggtggag aaagctgcaa tgacgatacc tgcaccgctg
    37861 tttttcggca tatgaatttc ttcatagtgg tcaggctttt tatacgcttc gcctttctct
    37921 ttcatttccc agaatgcatc acgctcgtga acgtgcggca caacggcaaa gttatagaac
    37981 ggaggcgggg aagaggttgc ccactccagc gtacggccac cccacgggtc gccagtcagg
    38041 tcacggttct ggtcgcggtc gcgaatagaa acgtacatct gaataacgag gcagagaata
    38101 cccagcgcaa tcagtactgc accgctagct gcaatcatca gcatggtgtg gaactgcggg
    38161 tcaatctgct ggctcaaacg acgggtcatg cccatgaagc ccagcgcata cagcggcata
    38221 aaggcaacga agaagccgat gatccagaac cagaacgcgc gtttacccca ggtttcattc
    38281 agtttgaaac cgaaggcttt aggccaccag taggtcatcc ctgcgaagca gccgaagacc
    38341 acgccgccga tgatcacgtt atggaagtgc gcaatcagga acaggctgtt atgcagaacg
    38401 aagtccgcgc ccggtactgc cagcagcacg ccagtcatcc cgcccaccga gaaagtgacg
    38461 ataaaaccga tggtccacag catcgcagaa tggaacacga tgcggccctg atacatggtg
    38521 aacagccagt tgaagatctt caccccggtc gggatggcga taatcattgt ggtgatacca
    38581 aagaaggcgt ttacgttcgc gcccgcaccc atcgtaaaga agtggtgcag ccaaacgatg
    38641 aacgacagca cggtgataca gacggttgcc cataccagcg aggtataacc aaacagacgt
    38701 ttacgcgaga aggttgccgc aatttcggag aacacaccga aaacaggcag gatcaggatg
    38761 taaacttccg ggtggcccca ggcccaaatc aggttgatgt acatcatcat gttgccaccc
    38821 atatcgttgg taaagaaatg ggtgcccaga tagcgatcca gggtcaacaa cgcgacggta
    38881 accgtcagaa ttgggaagga agcaataatc agtacgttcg cgcacagtga tgcccaggta
    38941 aatactggca tcttgaacat ggtcatgccc ggtgcgcgca tcttcagaat ggtaacgaag
    39001 aagttgatac cggtaagcgt cgtacctata ccggatagct ggagactcca tatccagtaa
    39061 tcgacaccga ctcccggact gtactctatt cccgatagcg gtggataggc cagccagccg
    39121 gtctgcgcaa attcgcccac gccgagagaa acgttaacca gaatcacacc aacaacagta
    39181 aaccagaagc ttaagttgtt gaggaacggg aacgcaacgt cacgcgcgcc gatctgcagc
    39241 ggaaccacca ggttcatcag accgataacg aaaggcatcg ccacgaagaa gatcataatc
    39301 acgccgtgcg cggtgaagat ctgatcgtag tggtgaggtg gcaggaagcc cgcttcgccc
    39361 gccgaggcaa gagcctgctg gctacgcatc ataatggcgt cagcaaaacc acgcagcaac
    39421 atcacaatcg ccacgatgat atacatgata ccgaggcgtt tatggtcgac ggaggtcagc
    39481 cactctttcc acaggtaggt ccacttaccg aagtaagtga tcaggccaac gagcgccaga
    39541 cctcccaaaa taatgccagc gatcgtaacc atgacgatag gttcatggaa cgggactgca
    39601 tcaagtgata attttccgaa catctttatt cttcctcaac ccctttaatg ggcggattcc
    39661 gcgtggctca tgtccatgcc ttccatacct tcgtgtgcac tgtgctcacc ttctggctgg
    39721 gtcatgtcca tgctcttacc gtgagccata aacttgttaa tcacatcggc aaacaagtct
    39781 ggtttcacgt tggagaaata ttccacctgg ttgtattcgc taggcgcggc cagtttttcg
    39841 aacgcagcca tgtcagacat ggtgttcggc gactgtttcg cttttgcgac ccattggtcg
    39901 aattcggcgc gatccggtgt tgcaatagct ttgaacttca tgcctgagaa gcccggcccg
    39961 ctgtagctgg cggagatacc gtcataagta ccgggttcgt tggcgatcag atgcaggcga
    40021 gtctgcatac cggccatggc ataaatctgg ctacccagac gcggaatgaa gaaggagttc
    40081 atcacggagt tggaggtcac tttgaagtac accggagtgt tcgccgggaa agcgatttca
    40141 ttcacggtag caatgccctg ttccgggtag atgaagaacc atttccagtc catggaaacc
    40201 acttcgatgg taatgggctt ctcgtcgtgt gccagcggct tgctaggctc aagagcgtga
    40261 gtggttttcc aggtcagtac cgcaaggaag atgatgatta agataggtac cgtccagacc
    40321 acagcttcca ctttattgga gtgtgaccag ttcgggctgt acttagcatc tttattgctc
    40381 gcacgatact tccaggcgaa accaacagcc atcaagattg cgggaataac gacaatcaac
    40441 atcaggccaa atgccgtcag tatcagtgaa cgttgctcca gaccaatctg tcctttggga
    40501 tctaacagcg cagaattaca gccactgagc aatacagtgc ctgcaaataa tgacaaccat
    40561 cccaaacttt tattgtattt cctgagtctc atttaacgac ctcaattcca cgggatctgg
    40621 tggcgtttaa agtgtgtggg cattttacgg gaaggttaca ttactgtaaa catgattaaa
    40681 ttcctgttac ttggatttgg ctgtcaggtc acatatgttg caaaacatat caagttgatt
    40741 ttaagaagct gttgcaaaag ggcgttataa cgaaagggga gtgcagaagg agtttggacg
    40801 ggcaattggt ataaccaatc tggaataaaa caattgttta acaaataatt atcattatgg
    40861 tgacaattgg gtgggggaaa ataaagactc attagctgaa tcgtgtaaga aaaaatagaa
    40921 ttcataaatt gcgtcaataa tttccaaacg ctaagccgca caaaagaaca aatattatta
    40981 agcgatactg atgtttattt agccgtaata attacggcga gtgattacta cagctaaata
    41041 atatttacag attacgtcag atgcgttttt cgtagcgcca gataatccag caaaccacca
    41101 agcacgacgc cagaaagggc gactaaaacc ccgacttcca gcagagcagg caggaaagag
    41161 aagtgcgtca aatccagcgc gtccatcgtc aacagcagta accacacggc caacaggctg
    41221 acgcccgccg ccagtgtcca catggcaaag gcataacctg ccggatattc ggtacgggag
    41281 ataaagttgt catttactcg tgtatattca agcgtctggc ggcaaaccag cagcaaaata
    41341 agccccggta cggcagcggc gacggagaat agatagaatg tcgaccagcc gtgtgcttca
    41401 acaaaccaac ccgccacggg gccgacataa actcgcccta cagcggaaag cgctgagagc
    41461 agggcaaatt gagttgcgga aaatgactta ttacacagcg tcattaacag cgcgacaaag
    41521 gctgatgtgc ccatcccgcc acagaggttt tcgaaaaaga cggctgcgcc catgctgtag
    41581 agatgcttat cagtaatcga cagcagccag taaccagcgt tagacgcacc ttgtaaaatg
    41641 ccgaaaatca gcagcgcccg gaacagcgac aggcgctgca tcaaaatccc accgtacaat
    41701 gcgcccacaa tggtcgccag taagccaagc gttttgttaa caacgcctac ttcacccgca
    41761 tcaaacccga cgccgcgaat caaaaacgtg gttgtcaggc tcatggcgaa tgcgtcgccc
    41821 agcttataca gcacgataag aagcaaaata agccaggcat tattgcgacc aaagaaatct
    41881 cgcagaggtg caacaaccgc ttgttccagc gtttttggca caggaatggt gtcggtcggt
    41941 tctggtgcaa gcaacgtcgc gataatacag gggatcaaca gcgccgccat caaccagtac
    42001 atgccctgcc agcccagcca tttatctgcc agccacaggg ctaagccgcc ggaaaccagc
    42061 atccctaaac ggtaacccag aacgctgatt gccgcgcccg caccacgttc ttctgccggg
    42121 agcacatcgg ttttccacgc atcgaagaca atatcctggg aggcagagca aaaagcgatc
    42181 accactgcca gtgccgccat ccagcggagt tgggtgcctg gttcgagaaa acccatcgcc
    42241 gcaatggcga ctaataacag gatttgcgtg gcgagcagcc aaccgcgccg ccgcccaaaa
    42301 aatgggggcg tgtagcggtc catcagcggt gaccagagga atttaaaaac gtaagcctgg
    42361 cctaccagag agaagaaacc aatggttttg agatcgatat tctcgaccgt catccaggcc
    42421 tgtaaggtgc cggatgtcag ggcgagcggt agcccggaag caaaacccag gatcagcaat
    42481 atggctgaac gcggctgttg aaaaatacgt aaatattgac tggacatggg cgtctacagg
    42541 cccggcttgc accgggccag agggcagaaa ttaacgcgcg ttctgcttga tgaattcgtg
    42601 gatgctggtg tcctgagaca tatcagcgat ggtatcggtc agcacactgt taaccgcatc
    42661 ggcgatattt ttgttggagg cctggaacgc accttcaacg ttgtagctgg cacgatagtt
    42721 tttggtcatt ttgttgccat tctgcgcggt agcgatgatg gcgatatccg ctttggtagc
    42781 gatgttgtag cgcacgttgc cctgggacac gtcagcatac agttggctaa cgatgatttg
    42841 cagattaacc gggccattcg gaccaaccat gtaaccacgc gcggtcatct gtttttccag
    42901 cacttcttgc agcaggaaac gcagatcgcg ggaggcggtc agggtaacga tttgattatc
    42961 gcgggtgact tttgccagcg cctgatcggt acgctgatcg gcaccattaa tgcttacggt
    43021 gacgcccatc aggcttggat cctgctgtgg cagtgtaatc gtcggggaaa cttcaatagt
    43081 tgttggcggt tttgcgcatc ctgcaagcat aaacagagca actaacggga agaggatttt
    43141 tttgaacatg ttcgggctct cagagactct taagcgtgtt tggtaaaaat tcccgccatc
    43201 ataacattgc caacggcgag gggaagtggg taaggcatgt aaattcatca tgttgacgaa
    43261 ataatcgccc ctggcaaaag aaacactgat gcgaggcctg tgtttcaatc tttaaatcag
    43321 taaacttcat acgcttgacg gaaaaaccag gacgaaacct aaatatttgt tgttaagctg
    43381 caatggaaac ggtaaaagcg gctagtattt aaagggatgg atgacatctc agcgttgtcg
    43441 gaggagatat ttcatgatga tacgtgagcg gatagaagaa aaattaaggg cggcgttcca
    43501 acccgtattc ctcgaagtag tggatgaaag ctatcgtcac aatgtcccag ccggctctga
    43561 aagccatttt aaagttgtgc tggtcagcga tcgttttacg ggtgaacgtt ttctgaatcg
    43621 tcatcgaatg atttacagta ctttagcgga ggaactctct actaccgttc atgcgctggc
    43681 tctgcatact tacactatta aggagtggga agggttgcag gacaccgtct ttgcctctcc
    43741 tccctgtcgt ggagcaggaa gcatcgcgta aaaacgcatt tgcaactgtc ggcgcttttc
    43801 cagtatgttg ctaaagattt tatgaaaaac ggcctgcggg ccgttttgtt ttgtctggat
    43861 tttgcgcttt ttgcccagca ttcagacgaa aattgcccgg gaattgtgaa aaaatacgcg
    43921 acagcgcgca ataaccgttc tcgactcata aaagtgatgc cgctataatg ccgcgtccta
    43981 tttgaatgct ttcgggatga ttctggtaac agggaatgtg attgattata agaacatccc
    44041 ggttccgcga agccaacaac ctgtgcttgc ggggtaagag ttgaccgagc actgtgattt
    44101 tttgaggtaa caagatgcaa gtttcagttg aaaccactca aggccttggc cgccgtgtaa
    44161 cgattactat cgctgctgac agcatcgaga ccgctgttaa aagcgagctg gtcaacgttg
    44221 cgaaaaaagt acgtattgac ggcttccgca aaggcaaagt gccaatgaat atcgttgctc
    44281 agcgttatgg cgcgtctgta cgccaggacg ttctgggtga cctgatgagc cgtaacttca
    44341 ttgacgccat cattaaagaa aaaatcaatc cggctggcgc accgacttat gttccgggcg
    44401 aatacaagct gggtgaagac ttcacttact ctgtagagtt tgaagtttat ccggaagttg
    44461 aactgcaggg tctggaagcg atcgaagttg aaaaaccgat cgttgaagtg accgacgctg
    44521 acgttgacgg catgctggat actctgcgta aacagcaggc gacctggaaa gaaaaagacg
    44581 gcgctgttga agcagaagac cgcgtaacca tcgacttcac cggttctgta gacggcgaag
    44641 agttcgaagg cggtaaagcg tctgatttcg tactggcgat gggccagggt cgtatgatcc
    44701 cgggctttga agacggtatc aaaggccaca aagctggcga agagttcacc atcgacgtga
    44761 ccttcccgga agaataccac gcagaaaacc tgaaaggtaa agcagcgaaa ttcgctatca
    44821 acctgaagaa agttgaagag cgtgaactgc cggaactgac tgcagaattc atcaaacgtt
    44881 tcggcgttga agatggttcc gtagaaggtc tgcgcgctga agtgcgtaaa aacatggagc
    44941 gcgagctgaa gagcgccatc cgtaaccgcg ttaagtctca ggcgatcgaa ggtctggtaa
    45001 aagctaacga catcgacgta ccggctgcgc tgattgacag cgaaatcgac gttctgcgtc
    45061 gtcaggctgc acagcgtttc ggtggcaacg aaaaacaagc tctggaactg ccgcgcgaac
    45121 tgttcgaaga acaggctaaa cgccgcgtag ttgttggcct gctgctgggc gaagttatcc
    45181 gcaccaacga gctgaaagct gacgaagagc gcgtgaaagg cctgatcgaa gagatggctt
    45241 ctgcgtacga agatccgaaa gaagttatcg agttctacag caaaaacaaa gaactgatgg
    45301 acaacatgcg caatgttgct ctggaagaac aggctgttga agctgtactg gcgaaagcga
    45361 aagtgactga aaaagaaacc actttcaacg agctgatgaa ccagcaggcg taatttacgc
    45421 agcataacgc gctaaattcg cacaaaggcc cgtcaccgcc aggtggtggg cttttttttg
    45481 tcatgaattt tgcatggaac cgtgcgaaaa gcctctttcg gtgttagcgt aacaacaaaa
    45541 gattgttatg cttgaaatat ggtgatgccg tacccataac acagggacta gctgataatc
    45601 cgtccataag gttacaatcg gtacagcagg ttttttcaat tttatccagg agacggaaat
    45661 gtcatacagc ggcgaacgag ataactttgc accccatatg gcgctggtgc cgatggtcat
    45721 tgaacagacc tcacgcggtg agcgctcttt tgatatctat tctcgtctac ttaaggaacg
    45781 cgtcattttt ctgactggcc aggttgaaga ccacatggct aacctgattg tggcgcagat
    45841 gctgttcctg gaagcggaaa acccagaaaa agatatctat ctgtacatta actccccagg
    45901 cggggtgatc actgccggga tgtctatcta tgacaccatg cagtttatca agcctgatgt
    45961 cagcaccatc tgtatgggcc aggcggcctc gatgggcgct ttcttgttga ccgcaggggc
    46021 aaaaggtaaa cgtttttgcc tgccgaattc gcgcgtgatg attcaccaac cgttgggcgg
    46081 ctaccagggc caggcgaccg atatcgaaat tcatgcccgt gaaattctga aagttaaagg
    46141 gcgcatgaat gaacttatgg cgcttcatac gggtcaatca ttagaacaga ttgaacgtga
    46201 taccgagcgc gatcgcttcc tttccgcccc tgaagcggtg gaatacggtc tggtcgattc
    46261 gattctgacc catcgtaatt gatgccagag gcgcaactgt gccgctatac ttatccaggg
    46321 cggcacaacg ctgtaagcgg cttgcgcctg agaatggcat ttgcgtcgtc gtgtgcggca
    46381 caaagaacaa agaagaggtt ttgacccatg acagataaac gcaaagatgg ctcaggcaaa
    46441 ttgctgtatt gctctttttg cggcaaaagc cagcatgaag tgcgcaagct gattgccggt
    46501 ccatccgtgt atatctgcga cgaatgtgtt gatttatgta acgacatcat tcgcgaagag
    46561 attaaagaag ttgcaccgca tcgtgaacgc agtgcgctac cgacgccgca tgaaattcgc
    46621 aaccacctgg acgattacgt tatcggccag gaacaggcga aaaaagtgct ggcggtcgcg
    46681 gtatacaacc attacaaacg tctgcgcaac ggcgatacca gcaatggtgt cgagttgggc
    46741 aaaagtaaca ttctgctgat cggtccgacc ggttccggta aaacgctgct ggctgaaacg
    46801 ctggcgcgcc tgctggacgt cccgttcacc atggccgacg caaccacgct gaccgaagcc
    46861 ggttatgtgg gcgaagacgt tgaaaacatc attcagaagc tgttgcagaa gtgcgattac
    46921 gacgtacaga aagcgcagcg cgggattgtc tacatcgatg aaatcgacaa gatttctcgt
    46981 aagtcagaca acccgtccat tacccgagac gtttccggtg aaggcgtaca gcaggcactg
    47041 ttgaaactga tcgaaggtac ggtagctgct gttccaccgc aaggtgggcg taaacatccg
    47101 cagcaggaat tcttgcaggt tgatacctct aagatcctgt ttatttgtgg cggtgcgttt
    47161 gccggtctgg ataaagtgat ttcccaccgt gtagaaaccg gctccggcat tggttttggc
    47221 gcgacggtaa aagcgaagtc cgacaaagca agcgaaggcg agctgctggc gcaggttgaa
    47281 ccggaagatc tgatcaagtt tggtcttatc cctgagttta ttggtcgtct gccggttgtc
    47341 gcaacgttga atgaactgag cgaagaagct ctgattcaga tcctcaaaga gccgaaaaac
    47401 gccctgacca agcagtatca ggcgctgttt aatctggaag gcgtggatct ggaattccgt
    47461 gacgaggcgc tggatgctat cgctaagaaa gcgatggcgc gtaaaaccgg tgcccgtggc
    47521 ctgcgttcca tcgtagaagc cgcactgctc gataccatgt acgatctgcc gtccatggaa
    47581 gatgtcgaaa aagtggttat cgacgagtcg gtaattgatg gtcaaagcaa accgttgctg
    47641 atttatggta agccggaagc gcaacaggca tctggtgaat aattaaccat tcccatacaa
    47701 ttagttaacc aaaaaggggg gattttatct cccctttaat ttttcctcta ttctcggcgt
    47761 tgaatgtggg ggaaacatcc ccatatactg acgtacatgt taatagatgg cgtgaagcac
    47821 agtcgtgtca tctgattacc tggcggaaat taaactaaga gagagctcta tgaatcctga
    47881 gcgttctgaa cgcattgaaa tccccgtatt gccgctgcgc gatgtggtgg tttatccgca
    47941 catggtcatc cccttatttg tcgggcggga aaaatctatc cgttgtctgg aagcggcgat
    48001 ggaccatgat aaaaaaatta tgctggtcgc gcagaaagaa gcttcaacgg atgagccggg
    48061 tgtaaacgat cttttcaccg tcgggaccgt ggcctctata ttgcagatgc tgaaactgcc
    48121 tgacggcacc gtcaaagtgc tggtcgaggg gttacagcgc gcgcgtattt ctgcgctctc
    48181 tgacaatggc gaacactttt ctgcgaaggc ggagtatctg gagtcgccga ccattgatga
    48241 gcgggaacag gaagtgctgg tgcgtactgc aatcagccag ttcgaaggct acatcaagct
    48301 gaacaaaaaa atcccaccag aagtgctgac gtcgctgaat agcatcgacg atccggcgcg
    48361 tctggcggat accattgctg cacatatgcc gctgaaactg gctgacaaac agtccgttct
    48421 ggagatgtcc gacgttaacg aacgtctgga atatctgatg gcaatgatgg aatcggaaat
    48481 cgatctgctg caggttgaga aacgcattcg caaccgcgtt aaaaagcaga tggagaaatc
    48541 ccagcgtgag tactatctga acgagcaaat gaaagctatt cagaaagaac tcggtgaaat
    48601 ggacgacgcg ccggacgaaa acgaagccct gaagcgcaaa atcgacgcgg cgaagatgcc
    48661 gaaagaggca aaagagaaag cggaagcaga gttgcagaag ctgaaaatga tgtctccgat
    48721 gtcggcagaa gcgaccgtag tgcgtggtta tatcgactgg atggtacagg taccgtggaa
    48781 tgcgcgcagc aaggtcaaaa aagacctgcg tcaggcgcag gaaatccttg ataccgacca
    48841 ttatggtctg gagcgcgtga aagatcgcat ccttgagtac cttgcggttc aaagccgtgt
    48901 caacaaaatc aagggaccga tcctctgcct ggtagggccg ccgggggtag gtaaaacctc
    48961 tcttggtcag tccattgcca aagccaccgg gcgtaaatat gtccgtatgg cgctgggcgg
    49021 cgtgcgtgat gaagcggaaa tccgtggtca ccgccgtact tacatcggtt ctatgccggg
    49081 taaactgatc cagaaaatgg cgaaagtggg cgtgaaaaac ccgctgttcc tgctcgatga
    49141 gatcgacaaa atgtcttctg acatgcgagg cgatccggcc tctgcactgc ttgaagtgct
    49201 ggatccagag cagaacgtag cgttcagcga ccactacctg gaagtggatt acgatctcag
    49261 cgacgtgatg tttgtcgcga cgtcgaactc catgaacatt ccggcaccgc tgctggatcg
    49321 tatggaagtg attcgcctct ccggttatac cgaagatgaa aaactgaaca tcgccaaacg
    49381 tcacctgctg ccgaagcaga ttgaacgtaa tgcactgaaa aaaggtgagc tgaccgtcga
    49441 cgatagcgcc attatcggca ttattcgtta ctacacccgt gaggcgggcg tgcgtggtct
    49501 ggagcgtgaa atctccaaac tgtgccgcaa agcggttaag cagttactgc tcgataagtc
    49561 attaaaacat atcgaaatta acggcgataa cctgcatgac tacctcggtg ttcagcgttt
    49621 cgactatggt cgcgctgata acgaaaaccg tgtcggtcag gtaaccggtc tggcgtggac
    49681 ggaagtgggc ggtgacttgc tgaccattga aaccgcatgt gttccgggta aaggcaaact
    49741 gacctatacc ggttcgctcg gcgaagtgat gcaggagtct attcaggcgg cgttaacggt
    49801 ggttcgtgcg cgtgcggaaa aactggggat caaccctgat ttttacgaaa aacgtgacat
    49861 ccacgtccac gtaccggaag gtgcgacgcc gaaagatggt ccgagtgccg gtattgctat
    49921 gtgcaccgcg ctggtttctt gcctgaccgg taacccggtt cgtgccgatg tggcaatgac
    49981 cggtgagatc actctgcgtg gtcaggtact gccgatcggt ggtttgaaag aaaaactcct
    50041 ggcagcgcat cgcggcggga ttaaaacagt gctaattccg ttcgaaaata aacgcgatct
    50101 ggaagagatt cctgacaacg taattgccga tctggacatt catcctgtga agcgcattga
    50161 ggaagttctg actctggcgc tgcaaaatga accgtctggc atgcaggttg tgactgcaaa
    50221 atagtgacct cgcgcaaaat gcactaataa aaacagggct ggcaggctaa ttcgggcttg
    50281 ccagcctttt tttgtctcgc taagttagat ggcggatcgg gcttgccctt attaaggggt
    50341 gttgtaaggg gatggctggc ctgatataac tgctgcgcgt tcgtaccttg aaggattcaa
    50401 gtgcgatata aattataaag aggaagagaa gagtgaataa atctcaattg atcgacaaga
    50461 ttgctgcagg ggctgatatc tctaaagctg cggctggccg tgcgttagat gctattattg
    50521 cttccgtaac tgaatctctg aaagaagggg atgatgtagc actggtaggt tttggtactt
    50581 ttgccgttaa agagcgtgct gcccgtactg gccgcaaccc gcagaccggt aaagagatca
    50641 ccatcgctgc tgctaaagta ccgagcttcc gtgcaggtaa agcactgaaa gacgcggtaa
    50701 actaagcgtt gtccccggtg gggatgtgac gaagttcaag ggcgcatcta ctgatgtgcc
    50761 ttttttattt gtattcggtg actttctgcg tcttgtgggc tgacaattgc ccccgtttct
    50821 tgtcacaata ggcctttgcg cgcatcgata cgttgcgtga ggtacacagt catctacagc
    50881 ggagtgttgt tacaccatga tggacagctt acgcacggct gcaaacagtc tcgtgctcaa
    50941 gattattttc ggtatcatta tcgtgtcgtt catattgacc ggcgtgagtg gttacctgat
    51001 tggcggaggc aataactacg ccgcaaaagt gaatgaccag gaaatcagcc gtgggcagtt
    51061 cgagaacgcc tttaacagcg agcgtaatcg catgcagcaa cagctgggcg atcaatactc
    51121 cgagctggca gcgaacgaag gctatatgaa aaccctgcgt caacaggtgc tgaatcgtct
    51181 gatcgacgag gcgctgttgg atcagtacgc tcgtgagctg aaactgggta tcagtgatga
    51241 gcaggttaaa caggcgattt tcgcgacccc agccttccag gttgacggca aatttgataa
    51301 cagccgctat aacggtatcc tcaaccagat ggggatgacc gccgatcagt acgcccaggc
    51361 gctgcgtaac cagctcacta cccaacagct gattaacggt gttgccggta ccgattttat
    51421 gctgaaaggt gaaaccgacg agctggcggc actggtcgcg caacaacgcg tggtgcgtga
    51481 ggcgactatc gatgttaacg cgctggcggc gaagcagcct gtgaccgaac aggagattgc
    51541 cagctactac gaacaaaaca aaaacaattt catgacgccg gaacaattcc gcgtgagtta
    51601 catcaagctg gatgccgcaa cgatgcagca accggttagc gatgcggata tccagagcta
    51661 ctacgatcag catcaggatc aattcaccca gccgcagcgt acccgctaca gcatcatcca
    51721 gaccaaaact gaagatgaag cgaaagcggt acttgatgag ctgaataaag gcggtgattt
    51781 tgctgcatta gccaaagaaa aatctgccga tattatctct gcccgtaacg gcggcgatat
    51841 gggttggtta gaagatgcca ctatccccga cgaactgaaa aatgctggtc tgaaagaaaa
    51901 agggcaactg tctggtgtca tcaaatcttc ggtcggtttc ctgattgtac gtctggacga
    51961 cattcagcca gcgaaagtga aatcgttaga cgaagtacgt gacgacattg cggcgaaagt
    52021 gaaacacgaa aaagccctcg atgcgtacta tgcgctgcag cagaaagtga gcgatgcggc
    52081 aagcaacgac acggagtctc tggccggtgc agagcaagct gccggcgtta aagccactca
    52141 gacgggttgg tttagcaaag ataacctgcc ggaagagttg aacttcaagc cggttgccga
    52201 cgctatcttt aacggcggtc tggtaggtga aaacggcgcg ccgggcatca actctgacat
    52261 catcaccgta gacggcgacc gcgcattcgt gctgcgcatc agcgagcaca aaccggaagc
    52321 ggtgaaaccg ttggcagatg ttcaggaaca agttaaggca ctggttcagc acaacaaagc
    52381 tgaacaacag gcgaaagtgg atgctgagaa actgctggtt gatttgaaag ccggcaaagg
    52441 tgcggaagct atgcaggctg ccggtctgaa atttggcgag ccgaaaatct taagccgttc
    52501 cggtcgtgac ccgattagcc aggcggcgtt tgcactgcca ctgccagcga aagacaaacc
    52561 gagctacggt atggcgaccg atatgcaagg taatgtggtt ctgctggcgc tggatgaagt
    52621 gaaacaaggt tcaatgccgg aagatcagaa aaaagcgatg gtgcagggta tcacccagaa
    52681 caacgcacaa atcgtctttg aagctctgat gagtaacctg cgtaaagagg cgaaaatcaa
    52741 aattggcgat gcgctggaac agcaataatc ctgaagccgc ctcgcaaaaa aatgcgttgc
    52801 agctgtaaca agaaaaggtc gctttcgcgg ccttttccat ttctgaacat tgccatttgt
    52861 ttactgtttt cactgccgtt aaggtgattc cactgttaac aaacaaggag aaaacagtat
    52921 gaaacacgga attaaagcac tgctcattac cctgtccctg gcctgtgccg gaatgtctca
    52981 tagcgcgctg gcggcagctt ctgtggcgaa accgacggcg gtagaaacca aagcggaagc
    53041 tcctgcagca caaagtaaag cagcagtacc ggcgaaagcc agtgacgaag aaggcacccg
    53101 ggtcagcatt aataatgcca gcgcggaaga gctagcccgc gcgatgaatg gcgttggcct
    53161 gaagaaagcg caggcgattg tcagttatcg cgaagagtac ggtccgttta aaacggtgga
    53221 ggatctaaag caggtgccgg ggatgggcaa ttcgctggtg gaacgtaatc tggcggtatt
    53281 aaccctgtaa ttaatttgca tagtggcaat tttttgccag actgaagagg tcataccagt
    53341 tatgacctct gtacttataa caacaacgta aggttattgc gctatgcaaa cacaaatcaa
    53401 agttcgtgga tatcatctcg acgtttacca gcacgtcaac aacgcccgct accttgaatt
    53461 tctcgaagaa gcccgctggg atgggttgga aaatagcgac agttttcagt ggatgacggc
    53521 ccataacatc gccttcgtcg tggtcaatat caatattaac tatcgtcgcc cagcggtatt
    53581 aagtgacctg ttaactatta ccagtcagtt gcagcaatta aacggtaaaa gcggcatctt
    53641 aagccaggtc attacactgg agccggaagg gcaggtggta gcggatgcgc ttattacgtt
    53701 tgtttgtatt gatcttaaaa cgcagaaagc attagctctg gaaggggaat tgcgcgaaaa
    53761 gctggagcag atggttaagt aaacgttttg tggtgccgga tgctcaagcc gcatccggcg
    53821 acacccggaa taattacctc aacccggttt tctgcttcat cgctgccatc accgtcggtt
    53881 tatcggccag ataatgattc aaaccgttgg cgcgtaaatt acatgccgca caatgaccgc
    53941 aaccgtcgcc tttaatgccg ttatagcagg tcaacgtttc gttacggact aaatccagtt
    54001 tgccgtaata atctgccagc gcccaggttt ccgctttatc aatccacatc agcggcgttt
    54061 caaaacgaat atctttcgcc atgcccaaac tgacggcatg gtttagtgct ttcacaaact
    54121 catcgcggca atccgggtag ccggagaaat ccgtttcaca gacgccggta attacggctt
    54181 ctgcttttac ctgatacgca tatattgccg ccagcgtcag gaacaaaata ttacgccctg
    54241 ggacaaacgt attcgggatg ccatcggctt caggttcata atcaggcacc ggaatgctgt
    54301 cacgtgtcag gctgctgacc gccagctcgt tgagcagcgt tacatcaaga accttatgcg
    54361 cgcgtgctcc cagtttcagc gccagttcgc gtgccacgtc gatttctgcg cgatgtcgct
    54421 gaccgtaatc gaacgtcacg caatggactt catcatattg ttgtaatgcc tgcaccagac
    54481 aggtggtgga atcctggcct ccactgaaca caacgacagc acgtttcata gataatcctg
    54541 cttgaacaat aaaagcgtta tggtaacgcc tgcgattaac ccggaccagc tattcattgc
    54601 gacggcgcgg gaagccaggc ttcggtaaat tcaaaccagc cgcgcggtgt cagtcgcaca
    54661 ccgttcacgc cgggaggggc actaatgcga tagtgatagt tgaacagcgg cgtcagcgtc
    54721 gcgtctgcca ttaactggct aaaaaccgct ttcagggcat taaatcggtt ttcctcatca
    54781 ggcattactt gcaccgcgtc cagcgtcgat tgcagatgtg catatgctgg agcgtcgaaa
    54841 acatgtggcc atagtggatc gcaacgtagc cattgctcca gagtatattc cggtgcttcg
    54901 ccaattaatc tgtcgcccat catgaggtct gcgtgtgcca gtagggtcgt gtcgtcccag
    54961 ttttttgcgt tatgaaaaat aattgtgagt tcacagcctt ccgctgccag tgtcgcctgt
    55021 aggcgttctg ccatggtatg aagttctatc ggtaagtgat aaaccagcgt caaggttttc
    55081 ggtagtttga cttcatccgg tacttgccag tgcggaatag tccagcctgg cagtaatgca
    55141 tgactggcgg tgatcaggtt ttctccgact tctaacgttt gtaataaacc ggattgatga
    55201 ataatggaga tcacttttcg cgcctgccag agggagagtc ggggactttt gcgcaacgtt
    55261 aaatagcaaa aacctaaact gatgccacta ctgacctggc tgacccgttg cagctcctcc
    55321 ggtttgccga tggtgatttg cacgggatgc cgacaactgg ttcccaaatc tttttcgaaa
    55381 agcggcggag ttatccagta ctcaaccgct ttaagcagcg gatgacgcaa atggtaataa
    55441 tcatggctct ccaggcgcac cagctccgct gtgaattgtg ttaagcgaaa aggaccagtg
    55501 ccgatcagtg ggaattgcgg atgcgccaga tggctgcaat agctcgccag ccggtgcgca
    55561 agccagtaat cggggcgatg taaaaagaag gtcagacact gcggatgggt gacttcaata
    55621 cgcttcacgc taataaataa ttgatccagt gctggcagtt gtaacagcat caataatcgc
    55681 tggtgtaagt gtgaggcttt tactgcatcg ccgttatgcc agtgtagggt tgaacgaaga
    55741 taaaagtccc agcgtaaccc gtcagtagag gtttcccagt gatgcgctaa atcgccaatc
    55801 gggcgctgag tattattatc gaagcgggtc aggccggaaa atatctgccc ggcgagatgc
    55861 tgctcggcac ggccgggcaa aaagcctggt tgtagcggtt cgagcgggcg atagtaggga
    55921 atacgcaacg tgggtgtatc gttttgccat tgtccgccca taaacggctg taacagagtg
    55981 cgcagctcac ctggggccag ttgcgccagc tccagcacat cttgctgctt tccggtttcc
    56041 agtgcctgtt ccatcatcgc attgcgtagc gattccggcg tgaccagaaa gcgtaattgt
    56101 ccgcgctttc cgcgtcctga ctgcgcctgc cactccagcc atcccgcctc ctgtgcctga
    56161 cgcaacagcg tacgaacatg gcgttcgctg caaaaacagc gttcggccag ttcgctgacg
    56221 gtgacggttt gcggctttcc ggctgaaggt tgccacagac gttgatactg gttaagacgg
    56281 ttgagcaatc gcatataaac ccggaacaat attatttaac tattcactat tacttccgta
    56341 tatatcaggt gatactcaat caccattaac cgtgtcacag agtggagaaa gaaatggctc
    56401 gtctggcagc atttgatatg gatggcactt tattgatgcc tgaccatcat ttaggtgaga
    56461 aaaccctctc tactttggcg cgactgcgtg aacgcgacat taccctcact tttgccacgg
    56521 ggcgtcatgc gctggagatg cagcatattc tcggggcgct atcgctggat gcatatttga
    56581 ttaccggcaa cggaacgcgc gtgcattctc tggaaggtga acttttacat cgtgatgatt
    56641 tacctgcgga tgtcgcggag ctggtgctgt atcagcaatg ggatacccga gccagcatgc
    56701 atatcttcaa tgacgacggt tggtttaccg ggaaagagat ccctgcgttg ttgcaggcat
    56761 ttgtctatag cggttttcgt tatcagataa tcgatgtcaa aaaaatgcca ctcggcagcg
    56821 tcaccaagat ctgcttctgt ggcgatcacg acgatcttac acgcttgcag atccagctat
    56881 acgaagcatt aggcgagcgt gcacatttgt gtttttccgc cacggattgc ctcgaagtgc
    56941 tgccggtggg ctgcaataaa ggcgctgcat tgacggtgct gacccaacat ttaggtttat
    57001 cgttgcgcga ttgcatggcc tttggtgatg cgatgaacga tcgcgaaatg ttaggcagcg
    57061 tcggtagcgg atttattatg ggcaatgcga tgccgcaact gcgcgcggag ctcccgcatt
    57121 taccggtgat tggacattgc cgaaatcagg ctgtctctca ctatttgacg cactggctgg
    57181 actatccaca tctaccttat tcccccgaat aacgagatcc cttccagcac cgggcaattg
    57241 cccggttttt tttgcgttga gtttgtcatt ttgtgccatg gtgtttaaac cgcacagaat
    57301 aaattgtcgc gatttcacct ttaaaataga attaaaagag aaaaaaattc tctgtggaag
    57361 ggctatgtta gataaaattg accgtaagct gctggcctta ctacagcagg attgcaccct
    57421 ctctttgcag gcactggctg aagccgttaa tctgacaacc accccctgct ggaagcgcct
    57481 gaaacggctg gaggacgacg gtatccttat cggcaaagtc gccctgctgg atccggaaaa
    57541 aataggcctc ggcctgaccg cctttgtgct gataaaaacg caacatcaca gcagcgaatg
    57601 gtattgccgc tttgtcacgg tggttaccga aatgccagaa gtgctggggt tctggcgcat
    57661 ggctggtgaa tacgattatc tgatgcgcgt ccaggttgcc gacatgaaac gctacgacga
    57721 gttttataag cgtctggtaa acagcgtgcc ggggctgtcg gacgttactt ccagcttcgc
    57781 gatggaacag attaaataca ccacttcttt acccatcgaa taaatatcca gaatcaggtc
    57841 aggacacaac gcgtgcgatt atttgctcaa ttaagctggt atttccgtcg ggaatggcgt
    57901 cgctatctcg gggctgtcgc cttgcttgtc attatcgcga tgctgcaact ggttccgcca
    57961 aaagtggttg gtattgttgt cgatggcgtg acagaacaac actttactac cgggcagatc
    58021 ctgatgtgga tcgccaccat ggtgctgatt gccgttgtgg tttatctcct gcgttacgtc
    58081 tggcgcgtat tgctgtttgg tgcgtcttat caactggctg ttgaactgcg tgaagattat
    58141 taccgtcagc taagccggca gcatcctgag ttttacctgc gtcatcgcac gggtgacctc
    58201 atggctcgtg cgactaatga cgtcgatcgc gtcgtgtttg ccgccggaga aggggtgctg
    58261 acgctggtgg attcactggt aatgggctgc gctgtgttga ttatgatgtc tacgcaaatt
    58321 agctggcagt tgaccttatt tgccctgtta ccgatgccag tgatggcgat catgattaag
    58381 cgcaacggcg atgccttgca tgaacgcttt aagctggcac aggcggcgtt ttctagtctt
    58441 aatgaccgca cccaggaaag cctcaccagt attcgcatga tcaaagcctt tggtctggaa
    58501 gatcgccagt cggcgttatt tgccgcggat gccgaagata ccggcaaaaa aaacatgcgg
    58561 gtggcgcgta ttgatgctcg tttcgacccg accatctata tcgcgattgg tatggcgaac
    58621 ttgctggcga ttggcggcgg tagttggatg gtggtgcagg gcagtttaac gctgggccag
    58681 ctcaccagtt ttatgatgta tttaggtctg atgatttggc caatgctggc gctggcatgg
    58741 atgtttaaca ttgtggaacg tggtagtgct gcgtacagcc gtattcgtgc gatgctggcg
    58801 gaagcgccgg tggtgaacga tggtagcgaa cccgtgccgg aagggcgtgg cgaacttgat
    58861 gtaaatattc accagttcac gtatccgcag actgaccatc ctgcgctgga aaacgtcaat
    58921 ttcgccctga aacccggtca gatgctgggt atctgcgggc cgactggttc cggcaaaagt
    58981 accctgttgt cgctcattca gcgtcatttc gacgtcagcg agggggatat tcgctttcat
    59041 gatattcctc tgacgaagtt acaactcgat agctggcgta gccgcctggc ggtagttagc
    59101 cagacaccgt tccttttttc cgacaccgta gcgaacaaca tcgcgttggg ttgcccgaat
    59161 gccacccagc aagagattga gcatgtcgcg cggttagcca gcgtacatga cgatattttg
    59221 cgtttaccgc aaggttacga tacagaggtg ggcgagcgcg gcgtgatgct ttccggcggg
    59281 caaaaacagc gtatctctat tgctcgtgcg ttattagtca acgcggaaat cctcatcctt
    59341 gatgatgcgc tttcggcggt ggacggacgc acagagcacc agatcctgca taacctgcgt
    59401 cagtgggggc agggaagaac ggtaatcatc agtgcccatc gcctttcggc actgacggaa
    59461 gccagtgaaa ttattgtgat gcagcacgga catatcgccc agcgtggcaa tcatgatgag
    59521 ctggcgcaac aaagcggctg gtatcgcgat atgtatcgct atcaacaact ggaggcggcg
    59581 ctcgacgacg ctccggaaat tcgcgaggag gccgtcgatg cgtagtttta gccaactgtg
    59641 gccgactctc aagcgtctgt tagcgtacgg ttcgccgtgg cgtaaaccgc tggggattgc
    59701 ggtcctgatg atgtgggttg cggcagcggc agaagtcagt gggccgctgc ttatcagcta
    59761 ttttatcgac aatatggtag cgaaaaataa cctgccgttg aaaatggttg cagggctggc
    59821 tgcggcgtat gttgggctac aactgtttgc cgccgggcta cattacgcgc agtcgctgct
    59881 gtttaatcgg gcggcagtag gcgtagtgca acagttgcgt accgacgtga tggatgccgc
    59941 attgcgccag ccattaagcg agtttgatac ccaacccgtc gggcaggtga tttcccgcgt
    60001 cactaatgac actgaagtga tccgcgatct ctacgttacc gtagtggcaa ctgtcctgcg
    60061 tagcgccgca ctggtgggcg cgatgctggt ggcgatgttc agcctcgact ggcgaatggc
    60121 gctggtggcg ataatgattt tcccggtggt gctggtggta atggtgatat accagcgtta
    60181 cagcacgccg attgtccgtc gtgtgcgcgc ctatctagcg gatatcaacg acggctttaa
    60241 cgaaatcatc aatggcatga gcgttatcca gcagttccgt cagcaggcgc gatttggcga
    60301 acgtatgggg gaggccagtc gttcacacta tatggccagg atgcaaaccc tgcgcctcga
    60361 cggttttctg ctgcgtccgc tgctgagtct gttttcatcg ctcattcttt gtggcttgtt
    60421 gatgctgttt ggcttctccg ctagcggcac cattgaagtg ggcgtgctgt atgcgtttat
    60481 cagctatctt gggcgactta acgaaccatt aatcgaactg accacgcaac aggcgatgct
    60541 gcaacaggct gttgttgctg gtgagcgcgt gtttgaactg atggacggac cgcgccagca
    60601 atatggcaat gatgagcgcc cgttacagag tggcaccatc gaagtcgata acgtgtcatt
    60661 tgcttatcgc gatgacaatc tggtgctaaa gaacattaat ctctctgtgc cttcgcgtaa
    60721 ctttgtggca ctggtcgggc ataccggcag tggcaaaagc accctcgcca gtttattgat
    60781 gggctattac ccgctaacgg aaggtgagat tcgccttgat ggtcgtccat taagttcgct
    60841 aagtcacagc gcgctgcgcc agggcgtggc aatggtgcag caagatccgg tggtgctggc
    60901 ggataccttc ctcgccaacg tgacgctggg gcgtgatatc tccgaagaac gcgtctggca
    60961 ggcgctggaa accgtgcaac tggcggagct ggcgcgcagc atgagtgatg gtatttatac
    61021 gccgctgggc gagcagggga ataatctctc cgtcgggcaa aagcaactgc tggcactggc
    61081 acgcgtgttg gtggagacgc cgcaaatcct gatccttgat gaggcaaccg ccagcattga
    61141 ctccggtact gaacaggcga ttcaacatgc tctggcggct gtgcgtgaac ataccacgct
    61201 ggtggtgatt gctcaccgct tatcgaccat tgttgatgcc gacaccattc tggtgcttca
    61261 tcgtgggcaa gccgtggagc agggcactca ccagcaactg ctggcggccc agggacgcta
    61321 ctggcagatg tatcaactgc aacttgcggg cgaagagctg gcagccagcg tgcgtgaaga
    61381 agaatcattg agcgcctgaa tagcgcaata tttcatcgtt ggtgcaaaaa tgtaacgcac
    61441 tgtgcactgt catagtgcgt tttcattttc aatcttctta acttcctgct ctctttctcg
    61501 tttttcattt ctggcacacc gcttgcaata ccttcttcgt gtagcagaac cattaccgaa
    61561 ttctgaccgg aggggatcta tgaagctggt gaccgtgata atcaaaccat tcaagctgga
    61621 agacgttcgt gaagcgttat cttccattgg tattcagggc ctgaccgtca ccgaagtgaa
    61681 aggtttcggg cgtcagaaag ggcatgccga gctgtaccgg ggggcggaat acagcgtcaa
    61741 cttcctgcca aaagtaaaaa ttgatgtggc gattgctgat gaccaactcg atgaagtgat
    61801 cgatatcgtc agtaaggcgg cttacaccgg aaaaattggc gacggcaaaa tcttcgtcgc
    61861 tgaattgcaa cgcgtcattc gtattcgtac cggcgaagcc gacgaagcgg cgctgtaatc
    61921 tctggcacac agcaacagga acgaaaaatg aagatagcga cgataaaaac tgggcttgct
    61981 tcactggcga tgctgccggg actggtaatg gctgcacctg cggtggccga taaagccgac
    62041 aatgcgttta tgatgatttg tactgcgctg gtgctgttta tgactattcc ggggattgcc
    62101 ctgttttacg gtgggttgat tcgcggcaaa aacgtgctgt cgatgctgac gcaggtgacg
    62161 gtgacgtttg cactggtctg tattctctgg gtggtttacg gttactcgct ggcgtttggt
    62221 gagggcaaca acttcttcgg caacattaac tggttgatgc taaaaaacat cgaactgacg
    62281 gcggtgatgg gcagcattta tcagtatatc cacgtggcgt ttcagggctc gtttgcctgc
    62341 attaccgtcg gcttgatcgt tggggcgctg gcggaacgaa tccgcttctc agctgtgttg
    62401 attttcgtgg tggtatggct gacgctctct tacattccga ttgcgcatat ggtgtggggc
    62461 ggtggtttgc tggcttctca cggtgcgctg gatttcgcgg gtggcacggt ggtgcatatt
    62521 aacgccgcaa tcgccggtct ggtgggcgcg tatctgatag gcaaacgcgt gggcttcggc
    62581 aaagaggcgt ttaaaccgca caacctgcca atggtcttca ccggtactgc cattctctat
    62641 attggttggt ttggctttaa cgccgggtca gcgggcacgg cgaatgaaat cgcggcactg
    62701 gcatttgtga atactgtggt cgcaacggcg gcggcaattc ttggctggat cttcggtgaa
    62761 tgggcgctgc gtggtaagcc ttctctgctg ggagcgtgtt ctggcgcgat tgccggtctg
    62821 gtcggcgtga cgccagcgtg tggctacatt ggggttggcg gcgcgttgat tatcggcgtg
    62881 gtagctggtc tggcgggctt gtggggcgtt actatgctca aacgcttgct gcgggtggat
    62941 gatccctgcg atgtcttcgg tgtgcacggc gtttgtggca ttgtcggctg tatcatgact
    63001 gggatttttg ccgccagttc gctgggtggc gtgggcttcg ctgaaggtgt gacgatgggc
    63061 catcagttgc tggtacagct ggaaagcatc gccattacga tcgtctggtc cggtgtggtg
    63121 gcatttattg gctacaaact ggcggatctg acggttggtc tgcgtgtacc ggaagagcag
    63181 gagcgagaag ggctggatgt caacagtcac ggcgagaatg cctataacgc gtaacaagca
    63241 ctgcaaaaaa cagccggacg gttttcacct ccggctattt ttttaattgt gattacgcat
    63301 caccccttcc tgaacggtcg aggcaaccag tacgccgtcc tgggtataaa actcaccgcg
    63361 cacaaagcca cgtgcgctgg acgccgaggt gctctccacg ctatacagca gccattcatt
    63421 caaattaaac gggcgatgga accacatgga atggtcaatg gtggcaatct gaatccccgg
    63481 ttcgagaaaa ccgatgccgt gcggctgtag agctaccggc aggaagttaa gatcagaagc
    63541 gtaaccgagc agatactgat gaacgcgcag gtcatccggc acgctaccat ttgcgcggat
    63601 ccacacctga cgatgtggtt ctgcgacgtg acctttcagt gggttatgaa actccaccgg
    63661 acggacttcc agcggacgat cgcagatgaa tttatcttta agcactggcg gcagcaggtg
    63721 cgccagcgat tgggcgattt gcgtttccga aggcaggcca tcaggcgctg gcgcggacgg
    63781 cattgttttt tgatgttcga aacccgcttc tggtgcctgg aaagaggccg tcatataaaa
    63841 aatcggtttg ccgttttgaa tagcagcaac ccggcgggcg ctgaagctgt taccgtcacg
    63901 cagcgtttcg acatcataaa taatcggctt cttactatcg cctgggcgaa gaaagtagct
    63961 gtgaaacgaa tgtaccagcc gctcttccgg gacggtctct tttgcagcat acaaggcctg
    64021 acccacgacc tggccgccaa acacctggcg taaacctaaa tcttcactct ggccgcgaaa
    64081 gagtccttcc tcaatttttt ccagatttaa caatgtcagt aaatttttga gcgcctgact
    64141 catataactc tccagtaaca aagctgccgc agcaagccaa agtgagttga gtataacgca
    64201 aatttgctac tggtccgatg ggtgcaataa tctgaattac gggctaatta caggcagaaa
    64261 tgcgtgatgt gtgccacact tattgacgtt actattttgt taaccactct tccggcgagg
    64321 aaagttagcc cgctggtgca ttgataataa ggagaaatga atgaaactcg tgcacatggc
    64381 cagtggttta gcggttgcga ttgcgttggc ggcttgcgca gataaaagcg cggatattca
    64441 gacgccagcc ccggctgcaa atacgtctat ttcagcaaca caacaacctg ctatccagca
    64501 accgaatgtc tccggtaccg tctggatccg gcagaaagtc gcactgccgc ctgatgctgt
    64561 gctgaccgtg acactttctg acgcgtcgtt agccgatgca ccgtcaaaag tgctggcgca
    64621 gaaagcggtg cgtaccgaag gtaaacagtc accattcagc tttgttctgc catttaatcc
    64681 ggcagatgtt cagccgaacg cgcgtattct gttgagtgcg gcgattaccg tgaatgacaa
    64741 actggtattt attaccgata ccgttcagcc ggtgatcaac aagggcggaa ctaaagccga
    64801 cctgacattg gtgccggtgc agcaaaccgc cgtgccggtt caggccagcg gtggtgcaac
    64861 gactaccgta ccttcgactt caccaactca ggtgaatccg tcttcggcag ttccagctcc
    64921 tacgcaatat taagcgacgt taaccctctc cgcccggaga gggttagtag ttccagcgat
    64981 aacgctgcaa gtcgatttgc ccgcttcccg ataccatcac accttctgcc agtaatgcct
    65041 gtcgctgacg ctgtaaatcc ggtccggtta gcgaaattgt gccgtggcga ttaaccaccc
    65101 ggtgccaggg taaggtgctg ccttcaggga gacgctttaa cacaccgccc acctggcgcg
    65161 cggcgcgggg cgatcccgcc agtttcgcca catcaccgta agtggtgaca tagccttcgg
    65221 gaatagcggc gacgatttgc cagacgcgtt ggggaaatga atcttctttt tccatctttt
    65281 cttcctgagg taatttttca gcataatctg gaaaaacgcc cgagtgaagt cgcattgcgc
    65341 aagaaaccag catctggcac gcgatgggtt gcaattagcc ggggcagcag tgataatgcg
    65401 cctgcgcgtt ggttctcaac gctctcaatg ggggctctgt tggttctccc gcaacgctac
    65461 tctgtttacc aggtcaggtc cggaaggaag cagccaaggc agatgacgcg tgtgccggga
    65521 tgtagctggc agggccccca cccatttctg cctcccacca tttcgtcaaa aaaccccaac
    65581 atggctaaac tttaaccaca actgacgtcg caagaattgt ctggctgcgc agtacgcttc
    65641 ggaggtatgt ctgatgaagt atgttgatgg ttttgtggtt gccgttcctg ccgataaaaa
    65701 ggatgcctat cgggaaatgg ccgctaaggc tgcgccattg tttaaagagt ttggcgcgct
    65761 tcgtattgtc gaatgctggg ccagcgatgt accggatggc aaagtgaccg attttcgtat
    65821 ggcggtgaaa gcggaagaga atgaagaggt tgtctttagc tggattgaat acccttcaaa
    65881 agaggtccgc gacgctgcta atcaaaagat gatgtcggac ccacggatga aagagttcgg
    65941 cgagtccatg ccgtttgacg gcaagcgaat gatctatggc ggattcgagt caatcatcga
    66001 cgaataggta gcgtgacggg ctgtgcggcg tgccgtcagc ccaaaaattc acaaatgttg
    66061 ctcggcccag cgtaagaaat cttctttcgg taatgccttg ctgtagagcc agccctgacc
    66121 gtagtgcacg ccatgctggc gtagccactc ttcttgttta ctggtttcga ttccctccgc
    66181 taccattttc agtttcagtg tttttgccat ttcgatgata tgcggcgtga catttttata
    66241 ttccagcgca tcaacgaaag atttatcgat cttcagaatg tcgacatcca gatcctgtaa
    66301 ataacttaaa cttgaatatc ccgtaccaaa atcatcaaga taaatttcat ggcccgcctc
    66361 ccggtagcga gaaattatcg gggcgctggt tttcggatcg gcaaactcgc gttcagtgag
    66421 ttcaagcgcg atctgtctgg gattaacctg atagtgattg atcatttcac gcagcaattg
    66481 cgggattttt tccgaggtga gcacggtgga ttcaagattg atcgaaatat gctgctgtgg
    66541 atgctgacgc agccagtcgc ccatatcttc aaagacgctt cttataatca gtagcgtcaa
    66601 tggctcagaa aggcccgttt gctgtgccag cggaataaaa ctatctggtg acaaccaact
    66661 accgtctgtc tgcggccagc gcgccagtgc ctcagcaccg acaattttgc cattggctaa
    66721 ggagacaatc ggctgatagt gcacgcaaat atcacgattt tcgatagcat cctgcagccg
    66781 atgatgcggt gactggatac ggcgcaaaat acgcagcaca aacatcgctg ccagcaggcc
    66841 aatcaccaac ccgagcggta accaaataaa gacttgccga tgccagcctt tctgcaacat
    66901 tttcgttgaa gcccaggtga tgatcgaaat attcatctcc gggaagggca ggatatcgta
    66961 aatgattcca ttattttcga tatgctcacc gggtgttttt tgtagcctgg taataattcc
    67021 ctgagcaatt tcatcgctgc tggttatgac aacgttatgg gcattgccaa taatggcggc
    67081 atcaatttgc catgagctat agggaatgac atcaataaag gaagcggggt cgatcatgac
    67141 gacataatgt gccgttccca tggcgaccat gtaacggata atgcctaaat cgttatgcga
    67201 tgttaaccag acacgataac catctttcga aattttacct ggctcgggga aggtatcggg
    67261 cggactttca tgctccagag acgaacactg gggaacgttg ttatcgatat aaaccacttc
    67321 ctgaatatag cgataactgt aagatacccg acgcatttcc atgagatggg cttcgctaca
    67381 ggcagcgcct tgccatcttt ccagctcttg cagcgcatct ttcccttgtg tcgccacctt
    67441 attggcccga atagcgacgc gggaggaata ggtatccagc tcttcaataa acgatgtttc
    67501 tacctgctga tgggccagcc agatgcttaa gccgacaggc agcaatactg aaagaatcag
    67561 tactcccgaa ataaggccga ccagatgtcg tgttctcacc agcatatcct tattaacaca
    67621 gtgtagggaa agtatatctg attgtggaaa aaggctggca ggagataagg aggtagagct
    67681 ggtatttcat gaagtgatgg gtggaaacga tcattgtcat ttgaccaggc gcagagcttt
    67741 acgcctggta tgtaacccta acttaagatg ttgattcggc gcgagcgagg gtaaaaatat
    67801 cgtaaaaaga cagttcacct ttacggacga tcattttttg cagttgttcc ttatgaacat
    67861 ccgcgacacg cggagagttc aagatggctt caaccagcgc ggcaggtaca aagcgcgtgt
    67921 tgtaccattc gccgttatag caaacgcgga aatccagcac gtcgatatct tcataacggt
    67981 agcgtgggta gacatcgaaa aagaagaaac cattaagcag gataaataat acaaccagta
    68041 gccagacaga gcctgacagc gtttcggact gcaacatcac cgccagggtg gcaaaaaaag
    68101 caacgtacat accgataaac agccccggat gtttacggat aaaactgata ctaaagcggg
    68161 gtttgttgtc gcgtttttcg cgggtattca gagactcaat cgtttcggtc agcaggcgtt
    68221 gtatttcggt catcgtttgc cttcgtggta tctgcaactt tccagggaac accctatttt
    68281 aacgtgggaa tagtagaggg gaagtaacag atttaccaca attttgcata acagttgcga
    68341 aaaaccgatt acaatttttt aattattctg gctggattac cgcccacgac aacgttgttc
    68401 gggacatctt ttgtgacaac tgcacctgag gctaccacga cgttatcacc aatggtcaca
    68461 ccagggttaa tgaccgcgcg tccgccaatc cagacgttat taccgatggt gacgggtttc
    68521 cccagttcag caccgctatt acgtgctaca gggtcgatgg gatgtgttgc cgtgtagata
    68581 tgaacgcctg gtgccaacat acagttatca ccgatgcgaa tagggcagac atcaagcatc
    68641 acgcaatcga agttggcgaa aaaattatta ccgagaaaaa tgttatagcc atagtcacag
    68701 cgaaacgttg gctcaatata agcctctgtc acctgaccga atagatcagc gagaatttgc
    68761 tggcgtaatg tgtgctcttc cgccagggaa tgattgtatc ggtgaataag ctgacgagcg
    68821 cgcaggcgat cgcgagataa cgtctcatct gccgagcgat acaactcacc agcaatcatc
    68881 ttttcttttt ctgtgctcat tactgacctc cggttgctga aaatgcggca acacagtaag
    68941 acgaaaagtc agtgaagaga acgttcccat tacatctgtg tgataaagat cacatagggt
    69001 atgtcttcat ggcgaaaaag tataaaattc ttaataaaca gccggttata gctccgaaag
    69061 cgaattattt attagcgaat aaatttccat actgaggaag ggatcttgtc gtacagttta
    69121 ttcatggtca attcggcgag gcggtgatct gcggctgagt aaaataccgc cagttcatta
    69181 tctgataatt cgtatttatt tttctcgata acacgctcca gcgtgtcaat tgtctggcaa
    69241 cgacgtaaac gcattaaata atcggttttc gttaaaggtt tttcggacat acttctaccc
    69301 atggttgtaa taattctaac aagataaact cgcaggattc tctttcgtcg cattgacaaa
    69361 acaacggaat agtcgattac ctgacttccg ccatttctga agatcctgca tattaatacc
    69421 ataactactg aacaacataa aggtgtcatc cagatattcg tcgatctgct caatgagctt
    69481 attgtcttca ttatacttaa ttttgtaatt aagtgcgaag gtcgcaatat gctcaatcag
    69541 ttcattcaac tggaggttga tcgccgaggt tgggtcgtta acccagccat gattgctttc
    69601 ttcaaggttt gcaaggcagt catgatacag ggtttcacag agaaacttaa gctgtgcgat
    69661 atcatgtctt ttgggtgagt attcatccat aacgcgtccc cttcttagcg gttgaactaa
    69721 cggacacctt tcgggatgga aaaaacttac tgacctggac ttgccctctc gtttgttagc
    69781 ttaactataa gccactcttt gcaggttttc atcgcattat tacgaaaaat tacaattagg
    69841 aaggaatcgg gacaggactc actttttcat actagcacta acccgcagca ggtaaaagca
    69901 gtctgctcga actgttcata acatttcagg attatcgcca gatgaaattg gtgacactaa
    69961 taccaggatt gctctgaata tgacgtaata accgaggaat gaataaagaa ttaccgcaaa
    70021 taattaaga
//
LOCUS       DAPGWD010000005        66957 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPGWD010000005 DAPGWD010000000
VERSION     DAPGWD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.005"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(114..2810)
                     /gene="mgtA"
                     /locus_tag="RHX88_000277"
     CDS             complement(114..2810)
                     /gene="mgtA"
                     /locus_tag="RHX88_000277"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDV2229011.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(2951..3004)
                     /gene="mgtL"
                     /locus_tag="RHX88_000278"
     CDS             complement(2951..3004)
                     /gene="mgtL"
                     /locus_tag="RHX88_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDV2229012.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            3189..4136
                     /gene="treR"
                     /locus_tag="RHX88_000279"
     CDS             3189..4136
                     /gene="treR"
                     /locus_tag="RHX88_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDV2229013.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            4255..5676
                     /gene="treB"
                     /locus_tag="RHX88_000280"
     CDS             4255..5676
                     /gene="treB"
                     /locus_tag="RHX88_000280"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDV2229014.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            5726..7381
                     /gene="treC"
                     /locus_tag="RHX88_000281"
     CDS             5726..7381
                     /gene="treC"
                     /locus_tag="RHX88_000281"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDV2229015.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQKINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            7775..9913
                     /gene="nrdD"
                     /locus_tag="RHX88_000282"
     CDS             7775..9913
                     /gene="nrdD"
                     /locus_tag="RHX88_000282"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDV2229016.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVKAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            10071..10535
                     /gene="nrdG"
                     /locus_tag="RHX88_000283"
     CDS             10071..10535
                     /gene="nrdG"
                     /locus_tag="RHX88_000283"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDV2229017.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNATQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(10580..10966)
                     /gene="cybC"
                     /locus_tag="RHX88_000284"
     CDS             complement(10580..10966)
                     /gene="cybC"
                     /locus_tag="RHX88_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDV2229018.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(11149..12501)
                     /gene="pmbA"
                     /locus_tag="RHX88_000285"
     CDS             complement(11149..12501)
                     /gene="pmbA"
                     /locus_tag="RHX88_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDV2229019.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDDAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            12595..13146
                     /locus_tag="RHX88_000286"
     CDS             12595..13146
                     /locus_tag="RHX88_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDV2229020.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(13297..14670)
                     /gene="mpl"
                     /locus_tag="RHX88_000287"
     CDS             complement(13297..14670)
                     /gene="mpl"
                     /locus_tag="RHX88_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDV2229021.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            14846..15844
                     /gene="fbp"
                     /locus_tag="RHX88_000288"
     CDS             14846..15844
                     /gene="fbp"
                     /locus_tag="RHX88_000288"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDV2229022.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(15877..16872)
                     /gene="yjfF"
                     /locus_tag="RHX88_000289"
     CDS             complement(15877..16872)
                     /gene="yjfF"
                     /locus_tag="RHX88_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDV2229023.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(16859..17881)
                     /locus_tag="RHX88_000290"
     CDS             complement(16859..17881)
                     /locus_tag="RHX88_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2229024.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(17895..19397)
                     /locus_tag="RHX88_000291"
     CDS             complement(17895..19397)
                     /locus_tag="RHX88_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV2229025.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(19707..20663)
                     /gene="ytfQ"
                     /locus_tag="RHX88_000292"
     CDS             complement(19707..20663)
                     /gene="ytfQ"
                     /locus_tag="RHX88_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDV2229026.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            20973..21503
                     /gene="ppa"
                     /locus_tag="RHX88_000293"
     CDS             20973..21503
                     /gene="ppa"
                     /locus_tag="RHX88_000293"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDV2229027.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(21883..22224)
                     /locus_tag="RHX88_000294"
     CDS             complement(21883..22224)
                     /locus_tag="RHX88_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDV2229028.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(22227..26006)
                     /gene="tamB"
                     /locus_tag="RHX88_000295"
     CDS             complement(22227..26006)
                     /gene="tamB"
                     /locus_tag="RHX88_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDV2229029.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(26003..27736)
                     /gene="tamA"
                     /locus_tag="RHX88_000296"
     CDS             complement(26003..27736)
                     /gene="tamA"
                     /locus_tag="RHX88_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDV2229030.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYSNGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            27942..28580
                     /gene="msrA"
                     /locus_tag="RHX88_000297"
     CDS             27942..28580
                     /gene="msrA"
                     /locus_tag="RHX88_000297"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDV2229031.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            28903..30246
                     /locus_tag="RHX88_000298"
     CDS             28903..30246
                     /locus_tag="RHX88_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV2229032.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(30308..30514)
                     /locus_tag="RHX88_000299"
     CDS             complement(30308..30514)
                     /locus_tag="RHX88_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDV2229033.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            30839..31396
                     /locus_tag="RHX88_000300"
     CDS             30839..31396
                     /locus_tag="RHX88_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV2229034.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(31386..32126)
                     /gene="cysQ"
                     /locus_tag="RHX88_000301"
     CDS             complement(31386..32126)
                     /gene="cysQ"
                     /locus_tag="RHX88_000301"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDV2229035.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            32316..34259
                     /gene="cpdB"
                     /locus_tag="RHX88_000302"
     CDS             32316..34259
                     /gene="cpdB"
                     /locus_tag="RHX88_000302"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDV2229036.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     LVVVLAHSGLSADPYKVMAENSVYYLSEIPGVDAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(34388..34768)
                     /locus_tag="RHX88_000303"
     CDS             complement(34388..34768)
                     /locus_tag="RHX88_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV2229037.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            34857..35717
                     /gene="qorB"
                     /locus_tag="RHX88_000304"
     CDS             34857..35717
                     /gene="qorB"
                     /locus_tag="RHX88_000304"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV2229038.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            35826..36791
                     /locus_tag="RHX88_000305"
     CDS             35826..36791
                     /locus_tag="RHX88_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2229039.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            36899..37561
                     /gene="ytfE"
                     /locus_tag="RHX88_000306"
     CDS             36899..37561
                     /gene="ytfE"
                     /locus_tag="RHX88_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDV2229040.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(37606..39018)
                     /gene="cycA"
                     /locus_tag="RHX88_000307"
                     /gene_synonym="dagA"
     CDS             complement(37606..39018)
                     /gene="cycA"
                     /locus_tag="RHX88_000307"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDV2229041.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(39327..39947)
                     /gene="fklB"
                     /locus_tag="RHX88_000308"
     CDS             complement(39327..39947)
                     /gene="fklB"
                     /locus_tag="RHX88_000308"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV2229042.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            40166..40795
                     /locus_tag="RHX88_000309"
     CDS             40166..40795
                     /locus_tag="RHX88_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HDV2229043.1"
                     /translation="MPGRFELKPTLVKIWHAPDNFRLMDPLPPMHRRGIIIAAIVLII
                     GILLPGDETRQMPVVTREAQLDMQSPPPGEAQLQAHLVTPQNDPDQVAPVAPEPIQEG
                     QPEEPPVAETQSFQPESGIDQQWRTYRVEPGKTLAQLFRDHNLPPTDVYAMAQVEGAD
                     KPLSNLQNGQIVKIRQNANGVVTGLTIDTGNNQQVLFTRQPDGNFIRAR"
     gene            complement(40796..41434)
                     /locus_tag="RHX88_000310"
     CDS             complement(40796..41434)
                     /locus_tag="RHX88_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDV2229044.1"
                     /translation="MYLDTSHAQNLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIREKERELDISGLSPVIAIEKIIDFTWDYYINNP
                     WFLKIVHSENQSKGVHYKKSKRLAEINHAHLQSMASLLEEGQRQKTFKKDIDPLQINI
                     NIAALGAYYLINQYTLGMVYHISMVSPEALEARRRVIKETILSWLLVNPARG"
     gene            41644..42435
                     /locus_tag="RHX88_000311"
     CDS             41644..42435
                     /locus_tag="RHX88_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDV2229045.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            42445..43287
                     /locus_tag="RHX88_000312"
     CDS             42445..43287
                     /locus_tag="RHX88_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV2229046.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASKMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            43297..44073
                     /locus_tag="RHX88_000313"
     CDS             43297..44073
                     /locus_tag="RHX88_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDV2229047.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEIEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            44083..45624
                     /locus_tag="RHX88_000314"
     CDS             44083..45624
                     /locus_tag="RHX88_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV2229048.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            45621..47702
                     /locus_tag="RHX88_000315"
     CDS             45621..47702
                     /locus_tag="RHX88_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDV2229049.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDKAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            47723..48199
                     /locus_tag="RHX88_000316"
     CDS             47723..48199
                     /locus_tag="RHX88_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDV2229050.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            48242..49543
                     /locus_tag="RHX88_000317"
     CDS             48242..49543
                     /locus_tag="RHX88_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDV2229051.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIVLIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            49596..50567
                     /locus_tag="RHX88_000318"
     CDS             49596..50567
                     /locus_tag="RHX88_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDV2229052.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(50681..51130)
                     /gene="rplI"
                     /locus_tag="RHX88_000319"
     CDS             complement(50681..51130)
                     /gene="rplI"
                     /locus_tag="RHX88_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDV2229053.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(51172..51399)
                     /gene="rpsR"
                     /locus_tag="RHX88_000320"
     CDS             complement(51172..51399)
                     /gene="rpsR"
                     /locus_tag="RHX88_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDV2229054.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(51404..51718)
                     /gene="priB"
                     /locus_tag="RHX88_000321"
     CDS             complement(51404..51718)
                     /gene="priB"
                     /locus_tag="RHX88_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDV2229055.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(51725..52120)
                     /gene="rpsF"
                     /locus_tag="RHX88_000322"
     CDS             complement(51725..52120)
                     /gene="rpsF"
                     /locus_tag="RHX88_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDV2229056.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            52447..52722
                     /locus_tag="RHX88_000323"
     CDS             52447..52722
                     /locus_tag="RHX88_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2229057.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(52851..53537)
                     /gene="ulaF"
                     /locus_tag="RHX88_000324"
     CDS             complement(52851..53537)
                     /gene="ulaF"
                     /locus_tag="RHX88_000324"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDV2229058.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(53537..54391)
                     /gene="ulaE"
                     /locus_tag="RHX88_000325"
     CDS             complement(53537..54391)
                     /gene="ulaE"
                     /locus_tag="RHX88_000325"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDV2229059.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(54401..55051)
                     /gene="ulaD"
                     /locus_tag="RHX88_000326"
     CDS             complement(54401..55051)
                     /gene="ulaD"
                     /locus_tag="RHX88_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV2229060.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(55065..55529)
                     /gene="ulaC"
                     /locus_tag="RHX88_000327"
     CDS             complement(55065..55529)
                     /gene="ulaC"
                     /locus_tag="RHX88_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDV2229061.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(55539..55844)
                     /gene="ulaB"
                     /locus_tag="RHX88_000328"
     CDS             complement(55539..55844)
                     /gene="ulaB"
                     /locus_tag="RHX88_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDV2229062.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(55860..57257)
                     /gene="ulaA"
                     /locus_tag="RHX88_000329"
     CDS             complement(55860..57257)
                     /gene="ulaA"
                     /locus_tag="RHX88_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV2229063.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            57612..58676
                     /gene="ulaG"
                     /locus_tag="RHX88_000330"
     CDS             57612..58676
                     /gene="ulaG"
                     /locus_tag="RHX88_000330"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDV2229064.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            58784..59539
                     /gene="ulaR"
                     /locus_tag="RHX88_000331"
     CDS             58784..59539
                     /gene="ulaR"
                     /locus_tag="RHX88_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDV2229065.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(59536..60285)
                     /gene="yjfP"
                     /locus_tag="RHX88_000332"
     CDS             complement(59536..60285)
                     /gene="yjfP"
                     /locus_tag="RHX88_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV2229066.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            60467..60796
                     /gene="bsmA"
                     /locus_tag="RHX88_000333"
     CDS             60467..60796
                     /gene="bsmA"
                     /locus_tag="RHX88_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDV2229067.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            60945..61220
                     /gene="yjfN"
                     /locus_tag="RHX88_000334"
     CDS             60945..61220
                     /gene="yjfN"
                     /locus_tag="RHX88_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDV2229068.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(61337..62962)
                     /locus_tag="RHX88_000335"
     CDS             complement(61337..62962)
                     /locus_tag="RHX88_000335"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDV2229069.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(63046..64209)
                     /locus_tag="RHX88_000336"
     CDS             complement(63046..64209)
                     /locus_tag="RHX88_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV2229070.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(64212..64850)
                     /locus_tag="RHX88_000337"
     CDS             complement(64212..64850)
                     /locus_tag="RHX88_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDV2229071.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(64860..65258)
                     /locus_tag="RHX88_000338"
     CDS             complement(64860..65258)
                     /locus_tag="RHX88_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDV2229072.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(65276..65935)
                     /locus_tag="RHX88_000339"
     CDS             complement(65276..65935)
                     /locus_tag="RHX88_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDV2229073.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(65986..66684)
                     /locus_tag="RHX88_000340"
     CDS             complement(65986..66684)
                     /locus_tag="RHX88_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDV2229074.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(66703..>66957)
                     /locus_tag="RHX88_000341"
     CDS             complement(66703..>66957)
                     /locus_tag="RHX88_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDV2229075.1"
                     /translation="MIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEE
                     YYIVFGALSLKSSLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT        16391 a        17751 c        16768 g        16047 t
ORIGIN      
        1 tcctgcaatt gattgaattt gtaggccgga taaggcattt acgccgcatc cggcatgaac
       61 aacgctcact ttgtctgcaa tctgaatcgg ggctatcgtg cccagtttat tctttactgc
      121 cagccgtaac gacggctata gaaccctttc accaactggg ttaacgtcat ataccctgcc
      181 agaatcgcaa ccagccacgg gaaatagctt aacggcagcg cctgtaattg cagataactg
      241 gccagcggtg aaaacggcaa tgcgatcccg acaatcatca cgatcacggt catgatcatt
      301 aacggccacg atgcacagct ctgaataaac ggcacacggc gggtacggat catatgcaca
      361 atcagcgttt gcgacagtaa gcccaccaca aaccatcccg actggaacag cgtttgcgtt
      421 tccggcgtgt tggcatggaa tacccaccac atcaggcaaa acgtcagaat atcgaagatc
      481 gagctgatcg gtccgaagaa gatcataaag cgccccagat ccgccggatt ccaacgctgc
      541 ggcttttgaa tttgctcgtc gtcgacgtta tcaaacggga tcgccacctg tgacacatca
      601 tacagcaggt tctgaataag caagtgtaac ggcaacatcg gcaggaaggg caagaaagca
      661 ctcgccacca gcacgctgaa cacattaccg aagttggagc tcgccgtcat tttgatgtat
      721 ttcagcatgt tggcgaaagt gcgacgtcct tcaataaccc cctcttccag caccatcagg
      781 cttttttcca gcaggatgat atcagccgct tcacgggcaa tatctaccgc gccgtccaca
      841 gaaatgccga tatccgcagc gcgtaaggcg ggcgcatcat taataccatc gcccataaag
      901 ccaaccacat gcccttcgcg cttcagtaag gtcacaatgc gttctttatg catcggcgtc
      961 aggcgggcaa acagcgtggt gcgctgtgcg agatttgcca gttcgtcgtc agatagcgtt
     1021 tcaatatcac taccaatgac cacctctccc gcgtccagcc ccacttcatg gcacactttc
     1081 gctgcaacta actcactgtc gccagtaagg atttttacgg tgattccact cgcttttaat
     1141 gccttcagtg ccggagcggt tgtctctttt ggcggatcaa ggaaagcaat atatccttcg
     1201 aggatcaggt cggattcatc cgcccgctgg taatctcctt cacgcgctgg caggtatttc
     1261 gtcgccaccg caaccacgcg cagcccctga cgattaagcg tatcagtaac ccgcttaatc
     1321 ttacgcagca tgatgtcatc gagcggtaca atctcgccat tgtgacgcac ctgcgaacac
     1381 acattgagga tttcctgcaa tgcacctttg caaaccagct gatggtgctc ggtattttct
     1441 gccactacca ccgacatccg gcgacgctcg aaatcaaacg gaatctcatc aattttttgc
     1501 caacgactgg ccagcgagcg cgctgactct tcatccgtac cttcgagcac cgctgtatcg
     1561 agcaggtttt taagtccggt ctgataatga ctgttcaacc acgcgctatg cagcacgcgt
     1621 tcgctggttt taccggagat atcggtatga ttctccagca caattttgtc ctgtgtcagg
     1681 gtgccagttt tatcagtgca cagaatatcc attgcgccaa agttctgaat agcatccaga
     1741 tgtttgacga tcactttctg tttcgacagc tttactgcgc cgcgcgccag cgtcgaggta
     1801 acaatcatcg gcaacatttc cggcgttaag cctaccgcga ccgaaagcgc aaacagtgcc
     1861 gcttcccacc agtcgccttt ggtgtaaccg ttgataagca acaccaccgg agccatcacc
     1921 agcataaagc gaatcagcag catactgacg cggctgatcc cctgctgaaa ggcattcggc
     1981 tcgctttcct gctcactaac acgccccgcc agttgaccaa accaggtatt ggcacctgta
     2041 gcaatcacca ttgcttgtgc cgtgccgctc accacggtgg tgcccataaa acacagcgtg
     2101 tcgcactcca gcggattgct gtgctccggc tggcgagtgg tagcggcttt ttccacgggc
     2161 agagactcac cggttaacga cgcctgagcg acgaacagat cccgcgcctg caagatacgt
     2221 aaatctgccg ggatcatatc tcccgccgcc agttttataa tatcgccggg caccagctgg
     2281 tcgatcggga tctccagcca gccattttcg cctttgtcgt taattacgcg cagcaccgtc
     2341 gcggtattgc tgaccatcgc tttcagggca tctgccgctt tagtggaacg tgcttcctga
     2401 ataaagttca gtaacgttga aatagcgacc attagcgcaa taacgcccgc ggcaaataaa
     2461 tcttccgtgg cgtaagaaat agcgccgaga atggtgagta aaatattaaa tgggttgcga
     2521 tagcagaccc ataaatgtac ccaccacggc gacggttgtt gtgcgggtaa tttattttca
     2581 ccatgttgtt cgcgggcaga ttccacttcc gcctgattta acccttccgg atgggtgtcg
     2641 aacgttttcc ataattcttc ttcgggcatc accgccattt tcaggcaatg cgcacttaag
     2701 gacggcggga ccaccgtatt cactgtctgc tgcgcgccag gcaatggatc acgatgaacc
     2761 agacgggaag gtaaatggcg aatgagccgg gtaaagattt ctttaaacat aaggagtccc
     2821 tccgcactgt ctgaaacagt acagaaatgt cttcaggcgt ggcgaagcct tacctgaagg
     2881 taataaagct gagcagggac gttaaaacgt cacggcctta cgcatccggt aacgccgtga
     2941 aaagacgggc ttaccggaaa agttttaatc tccgtcgagg gagaggcgtg ggatcaggtt
     3001 ccatataacc tccggtaagt gaataaagcg ccgcaaataa taaggagaat ccaataatat
     3061 tcgtagcatc tatggcgata taatacctgc tggcaactaa accaacgata aaccagactt
     3121 tacccattgc tgaatgcacg ggtaaagtta ggctcaaata attaaacaac acgttacagg
     3181 acaacaggat gcaaaatcgg ctgaccatca aagacatcgc acgcttaagc ggcgtgggga
     3241 aatctacagt ttcccgggtg ctgaataacg aaagcggcgt gagccagcgc acccgcgagc
     3301 gtgttgaagc agtgatgaat cagcatggat tttccccttc ccgctctgcg cgcgctatgc
     3361 gtgggcaaag cgataaagtg gtcgccatca ttgttacccg cctggattcg ttgtcagaaa
     3421 atctcgccgt tcaaaccatg ctgccagcgt tctatgaaca aggttacgac ccaatcatga
     3481 tggaaagtca gttttccccg caattagttg ccgaacattt gggggtgctg aaacggcgta
     3541 atatcgacgg cgtagtgctg ttcggtttta ctggcataac agaagaaatg ttagcccact
     3601 ggcagtcatc gctggttctg ctggcgcgtg acgcaaaagg ttttgcttcg gtctgttatg
     3661 acgacgaagg ggcaatcaag atcctgatgc aacggctgta tgaccagggg catcgtaata
     3721 tcagttatct cggcgtgccg catagtgacg tgacgaccgg gaagcgacgt cacgaagcct
     3781 acctggcgtt ctgcaaagcg cataaactgc atcccgttgc cgctctgcca ggccttgcta
     3841 tgaagcaagg ctatgagaac gtagcaaaag tgattacgcc tgaaactacc gccttactgt
     3901 gcgcaaccga cacgctggca cttggcgcaa gtaaatacct gcaagagcaa cgcatcgaca
     3961 ccttgcaact ggcgagcgtc ggtaatacgc cattaatgaa attcctccat ccggagatcg
     4021 tcaccgtaga tcctggttac gccgaagctg gacgccaggc ggcctgccaa ttgatcgcac
     4081 aggtaaccgg gcgcagcgaa ccgcaacaaa tcatcatccc cgccaccctg tcctgatcgt
     4141 ttcctgaacg ataaattgtg atcttcgctg cgtttcggga acgttcccgt ttttaaattt
     4201 ttccgcgcaa tatattctgc agccaaccaa aaatgtcatc tgccatgggg ctttatgatg
     4261 agcaaaataa accaaacgga tatcgatcgg ttgattgaac tggtcggcgg gcgcggcaat
     4321 attgcgacgg tgagccactg tattactcgc ctgcgctttg tcctcaacca accggccaat
     4381 gccagaccga aagaaattga gcaactcccc atggtgaaag gctgtttcac caatgccggg
     4441 caatttcagg tggtgattgg caccaatgtg ggtgattact atcaagcact gattgcgtca
     4501 accggacagg cgcaggttga taaagagcag gtaaaaaaag ccgcccggca gaatatgaaa
     4561 tggcatgagc aattgatctc tcatttcgcg gagatcttct tcccgctgct gcccgcgttg
     4621 attagcggcg gtttgatcct cggttttcgc aatgtgatcg gcgatttgcc catgagcaac
     4681 ggtcagacgc tggcgcaaat gtacccttcc ctgcaaacga tctacgattt tctgtggttg
     4741 atcggtgaag cgatcttctt ctacctgccg gtcggtattt gctggtcagc ggtgaaaaaa
     4801 atgggcggca cgccgatcct tggtatcgtg cttggcgtga cactggtttc tccccagctg
     4861 atgaacgctt atctgctcgg gcagcagctg ccggaagtgt gggactttgg catgttcagc
     4921 atcgccaaag taggctatca ggcgcaggtg atcccggcac tgttagccgg gctggcgctg
     4981 ggcgttattg aaactcgcct taaacgcatc gtgccggatt acctctatct ggtggtggtg
     5041 cccgtctgtt cgctgatcct cgcggtgttc ctcgcccatg cgctgattgg tccgtttggt
     5101 cgcatgattg gcgatggcgt tgcctttgcg gtacgtcacc tgatgaccgg cagctttgct
     5161 ccgattggcg cagcattgtt tggcttcctg tacgccccgc tggtgatcac cggtgtacac
     5221 cagaccacgc ttgctattga tttgcagatg attcaaagca tgggtggcac gccagtgtgg
     5281 ccgctgattg cgctgtcgaa tatcgctcag ggctccgccg tgataggcat tatcatttcc
     5341 agccgcaagc acaatgaacg cgagatctcc gtgcctgccg ctatctccgc ctggcttggg
     5401 gtcactgagc ctgcaatgta cggcatcaac ctgaaatatc gcttcccgat gctgtgcgcg
     5461 atgattggtt ctggtctggc aggattacta tgcggcctga acggcgttat ggcgaatggt
     5521 atcggcgtag ggggcctgcc gggaattctc tcgattcaac cgagctactg gcaggtattt
     5581 gcgctggcaa tggctatcgc catcatcatc ccgattgtac tcacctcgtt tatctatcag
     5641 cggaaatacc gcctgggcac gctggacatt gtttaatttt cttcggggcg caattgcgcc
     5701 ccctcgcatt tgcaggaata acgtaatgac taatcttccc cactggtggc aaaacggcgt
     5761 tatctaccag atttatccaa agagttttca ggacaccacg ggtagcggca ccggcgattt
     5821 acgtggcgtt atccaacgcc tggactatct gcataaactg ggcgttgatg ccatctggct
     5881 aacccccttt tatgtctctc cccaggtcga taacggttac gatgtagcga actatacggc
     5941 gattgatccc acctacggca cgctggacga ttttgacgaa ctggtgacgc aggcaaaatc
     6001 gcgcgggatt cgtatcattc tcgatatggt gtttaaccat acctctaccc aacatgcctg
     6061 gtttcgcgag gcgctgaaca aagaaagccc ttaccgccag ttttatatct ggcgcgatgg
     6121 cgaaccagaa acgccaccga acaactggcg ttcaaaattt ggcggtagtg cgtggcgctg
     6181 gcatgcggaa agcgaacagt actatttgca tctctttgca ccagaacagg cggatctcaa
     6241 ctgggagaat ccagcggtac gcgcagagct gaaaaaagtc tgtgaattct gggccgatcg
     6301 tggggtcgac gggttgcgcc tggatgtggt gaatctgatc tccaaagacc cgcgtttccc
     6361 tgaagacctg gatggcgacg ggcgtcgctt ctacaccgac gggccacgag cacacgagtt
     6421 tttgcacgag atgaaccgcg atgtgtttac gccacgcggg ttaatgaccg taggtgaaat
     6481 gtcctccacc agccttgagc attgccagcg atacgcggca ctgacaggca gtgaattgtc
     6541 gatgaccttt aattttcatc acctgaaggt cgattatccc ggtggtgaaa aatggacgct
     6601 ggctaaacct gactttgtgg cgttgaaaac attgttccgc cactggcaac aaggaatgca
     6661 caacgtggca tggaatgcct tgttctggtg taaccacgat cagccgcgca ttgtttctcg
     6721 ctttggtgat gaaggtgaat accgcgtgcc tgcggcaaaa atgctggcga tggtgctgca
     6781 tggcatgcag ggaacgccgt atatctacca gggcgaagag attggcatga ccaacccgca
     6841 tttcacgcgc attactgact atcgcgacgt ggagagccta aatatgtttg ccgagctgcg
     6901 caacgatggt cgtgatgccg acgagttatt ggcaatcctc gccagtaaat cccgtgacaa
     6961 cagccgcacg cccatgcaat ggagcaacgg cgataatgcc gggtttacgg ctggcgaacc
     7021 gtggattggc ctgggcgata actatcaaaa aatcaacgta gaagccgcgc tggccgatga
     7081 gtcctcggtg ttttacacct accaaaagtt aatcgcactg cgtaagcaag aagccatcct
     7141 gacatggggc aattaccagg atctgctgcc aaacagccct gtattgtggt gctatcgccg
     7201 tgaatggaag gggcaaacct tgctggtcat tgccaacctt agccgtgaga tccaaccctg
     7261 gcagccaggg caaatgcgcg gcaactggca gcttgtgatg cataactacg aagaagcctc
     7321 accacaaccc tgtgccatga atttacggcc ttttgaggct gtctggtggt tacagaagta
     7381 aatcttccct aagccccggt aatgccggtc attccggggt tttgtctgtc agcatgttgt
     7441 ttttgttgat ttttcaacca gcaaattcat taaaaaattt acataacgct gtagcgcccg
     7501 tcatccgtac gctctgcttt ttactttgag ctacatcaaa aaaagttaaa acatccttga
     7561 tgcaaagcac tatatataga ctttaaaatg cgtccaaacc caatatgttg tattaatcga
     7621 ctataattgc tactacagct ccccacgaaa aaggtgcggc gttgtggata agcggatggc
     7681 gattgcggaa agcaccggaa aacgaaacga aaaaaccgga aaacgccttt cccaatttct
     7741 gtggataacc tgttcttaaa aatatggagc gatcatgaca ccgcatgtga tgaaacgaga
     7801 cggctgcaaa gtgccgttta aatcagagcg catcaaagaa gcgattctgc gtgcagctaa
     7861 agcagcggaa gtcgatgatg ccgattattg cgccactgtt gccgcggttg tcagcgagca
     7921 gatgcagggc cgcaaccagg tggatatcaa tgagatccag accgcagttg aaaatcagct
     7981 gatgtcaggt ccatacaaac aactggctcg tgcttacatc gagtaccgtc acgatcgcga
     8041 cattgaacgt gaaaaacgcg gtcgcctgaa ccaggagatc cgtggtctgg tcgagcagac
     8101 caactcctcg ttactcaacg aaaacgccaa caaagacagc aaggtgattc caacccagcg
     8161 cgacctgctg gccgggatcg tggctaaaca ctatgcacgc cagcacctgc tgccgcgtga
     8221 cgtggtgaag gcacatgagc gtggcgatat tcactatcac gatctcgatt actcgccgtt
     8281 tttcccgatg ttcaactgca tgttgatcga cctgaaaggc atgctgaccc agggctttaa
     8341 gatggggaac gccgagattg aaccgccgaa gtcgatctct acggcaaccg cggtaactgc
     8401 gcagattatt gctcaggtcg ccagccatat ttatggcggc accaccatta accgtatcga
     8461 tgaagtgctg gcgccgtttg ttaccgccag ctacaacaaa catcgcaaga ccgcagaaga
     8521 gtggaacatc ccggacgccg aaggctacgc aaactctcgt accatcaaag agtgctacga
     8581 tgccttccag tcgctggagt acgaagtaaa caccctgcac accgccaacg gtcagacgcc
     8641 gtttgtaacc tttggttttg gtctgggcac cagctgggaa tcgcgcctga ttcaggaatc
     8701 gatcctgcgt aaccgtatcg caggcctcgg taaaaaccgt aaaactgcgg tgttcccgaa
     8761 actggtgttt gcgattcgcg atggtctgaa ccataaaaaa ggcgatccga actacgacat
     8821 caaacagctg gcgctggagt gcgcaagcaa gcgcatgtat ccggatatcc tgaactacga
     8881 tcaggtagtg aaagtcaccg gttcgtttaa aaccccgatg ggctgccgca gcttcctcgg
     8941 cgtgtgggaa aatgaaaacg gcgagcagat ccacgatggt cgtaacaacc tcggcgtgat
     9001 cagcctgaac ctgccgcgta ttgctctgga agcaaaaggc gatgaagcca ccttctggaa
     9061 gctgctggat gaacgtctgg tgctggcacg taaggcgctg atgacccgta tcgctcgtct
     9121 cgaaggcgtg aaagcgcgcg tcgccccgat cctctatatg gaaggtgctt gtggcgtacg
     9181 tctgaatgct gacgatgatg tttctgaaat cttcaaaaac ggtcgtgcgt ctatttcgct
     9241 gggttacatc ggcatccacg aaaccattaa cgcgctgttc ggcggcgagc atgtttacga
     9301 caacgagcag cttcgcgcga aaggtatcgc cattgttgaa cgtctgcgtc aggcagtgga
     9361 tcagtggaaa gaagaaacgg gttatggttt cagtctctac agcacgccga gtgaaaacct
     9421 gtgcgaccgc ttctgccgtc tcgatactgc tgagtttggc gtggtgccgg gcgtgaccga
     9481 taaaggttac tacaccaaca gtttccacct cgatgtggag aagaaggtga acccgtacga
     9541 caagatcgac tttgaagcgc cttacccgcc gctggcgaac ggtggtttca tttgctacgg
     9601 cgagtatcca aacattcagc acaacctgaa ggcgctggaa gatgtctggg attacagcta
     9661 tcagcatgta ccgtattacg gcaccaatac accgattgat gagtgctacg agtgtggctt
     9721 taccggtgag ttcgagtgca ccagcaaagg cttcacttgc ccgaaatgtg gtaaccatga
     9781 cgcctcccgt gtgtcggtaa ctcgccgcgt gtgcggatat ttaggtagcc cggatgcacg
     9841 tccgtttaac gctggtaagc aggaagaagt taagcgccgc gttaaacatt tggggaatgg
     9901 gcagataggt taatcttcac tctgcgctaa gcaaatgccg gatgcggcgt aaacgcctta
     9961 tccggcctac atttggcaac ggctccaggc ctgataagac gcgacagcgt cgcatcaggc
    10021 attgattacc ggatgcagcc tacacgcgga ctgataagcc aggcgaaata atgaattatc
    10081 atcagtacta tcctgtcgac atcgtcaacg gccccggcac tcgctgcacc ctgtttgtct
    10141 ccgggtgtgt tcatgaatgc cccggttgct ataacaaaag cacatggcgg gtaaattctg
    10201 gtcagccatt taccaaagca atggaagacc agatcattaa cgatctgaat gacactcgta
    10261 tcaaacgcca gggtatttcc ctctccggcg gcgatccgct gcatccgcaa aacgtgccgg
    10321 atattctgaa actggtacaa cgcatccgcg ccgagtgtcc gggtaaagac atctgggtgt
    10381 ggacaggcta taaactcgac gaactcaacg ctacgcaaat gcaggttgtt gatttgatta
    10441 acgtgctggt cgacggcaag tttgtgcagg atttaaaaga cccttccctg atctggcgcg
    10501 gcagcagcaa tcaggtggtg catcatttgc gatgataagt tccctgttac ctgagtgcag
    10561 gcaacaggga aatgaggaat taacgatact tctggtgata ggcgttgcgg gtcgttttca
    10621 gttgctctgc ggcggcctgc gcttctttta ctttaccttc atttgccagc ttcagcgcgt
    10681 cgtcaatctg accgaccaga atgtcgaaac cgtggcggaa atctttcatt tccgggctgt
    10741 ccggtgattt atcttcgagc ttcggcggcg ttgctttttg cgcatccagc gctgcggcgc
    10801 gcatcatcgt taacgcgtct ttgacttgcg ccgcgttatc cgctttttcg atcactttta
    10861 aattgtcgtt gagggtttcc atattgtctt caagatcagc ggcaaacgac gcagaactga
    10921 ataccaacga ggagactgca agaatagcta acaggctttt acgcattgct cacttccttt
    10981 tttatttaca cttcatcctt cacactgcct catcgacggc tacgctcatt cgcccgggtc
    11041 acgtactgcg agtacgctcc ccggccctca ttcactttcc gtctcgatgc agtgcgaatg
    11101 attttgtgta attttttagt ttatagcgcg gcaggtcgcg ccagtttttt actgcccggc
    11161 gattttcatc tccggcaaca gcacagaacc acactgtata ttactgcgtg tttcaatatc
    11221 gttaccgacg gtgacaatat tgcgccacat atcttttaaa ttacctgcga tggtgatttc
    11281 actaaccgga tactgaattt cgccattctc tacccagaag cccgcagcac cacgggaata
    11341 atcaccagta atggcactca cgccctggcc catcagctcc gtcaccacca gcccggtgcc
    11401 catctctttg agcatttgtt cgaagcttag cccttgtccg gcaatccgcc agttgtgaat
    11461 accgcccgca tgtccggtgc ttttcagccc cagtttccgc gccgagtagc tggtcagcag
    11521 ccactgagtc aggatgccat ctttaataat atcgcgacgc tcggtgcgca caccttcgct
    11581 gtcgaatggc gtcgacgcca gccctttcag cagatgcgga tgctcttcaa tggtcagcca
    11641 gtccggcaga atttgtttac ccagcgaatc cagcagaaag gtagatttac ggtaaaccga
    11701 gccacccgct atcgccccca ccagatggcc aaaaagcccg gttgccactt cattggcaaa
    11761 aatgactggc gctttcatgg tggagagttt acgcggtgac aggcgcgata aagtacggcg
    11821 agcacagtcg gccccaaccc actctggcgt ttgcagatcg ctcatcgcac gaccaatggt
    11881 gtaggcgtaa tcacgctcca tatcgccatt ttcttcggca attacacagc tggagagtga
    11941 atgacgcgta gagcagtaac cctgcaacat gccgtggctg ttgccaaaaa ctttgacacc
    12001 gtagtggctg ttaaaactgc cgccttcggt attggtaatg cgtttgtccg cctgcaatgc
    12061 cgcctgttct gcgcgggccg ccagttcaat ggcatcatcc ggggaaactt cagcagggtg
    12121 gaacaagtcg agatctggtg cgtcaaaggc cagcagctct ttgtctgcca ggccggcaca
    12181 gggatctggc gaggtataac gggcaatatc cagcgccgcc tgtacagtac gggcaatggc
    12241 ctgcgggctt aaatcggtgg atgatgcgct acctttgcgg ttctggtgat aaacagtgat
    12301 ccccagcgcg ccatcgctat tgaattcaac attctccact tcaccataac gcgtgcttac
    12361 gctaatgccg gtggtcttgc tgacggcaac ttccgcaccg tccgatttgc ctgaggccaa
    12421 ctccagcgca gtggaaactg cttcttccag aatcttgcgc tgcgcttcaa cttgagagat
    12481 tactttcatt gcaagtgcca taatgtagag agtttctctg aagtctaaca gagaaccgtt
    12541 tttcagtgcg catcttaact ggtaacatta gcctcttttt ttaaggagcc tgagatgact
    12601 aagcagcccg aagactggct cgacgacgtt cccggtgatg acatcgaaga cgaagacgat
    12661 gaaattatct gggtcagtaa aagtgaaatt aaacgtgatg ccgaggagct aaaacgcctt
    12721 ggcgcggaaa ttgttgatct ggggaaaaac gcgctggata agatcccgtt agatgcggat
    12781 ctgcgcgcgg ctattgagct cgcccagcgt attaagatgg aaggtcgccg ccgccagttg
    12841 cagctcatcg gtaagatgct gcgccagcgc gacgtagagc ctattcgtca ggcgctggat
    12901 aagctgaaaa accgtcacaa ccagcaggtg gtgctgttcc ataaactgga aaacctgcgc
    12961 gatcgtctga tcgatcaagg tgatgacgcc atcgctgaag tattgaatct gtggcccgat
    13021 gccgatcgtc agcagctgcg tactctgatc cgtaacgcga agaaagagaa agaagggaat
    13081 aagccgccga aatccgcacg ccagattttc cagtatctgc gcgaactggc ggagaatgaa
    13141 gggtaatttt cgttgttgat gtgagtatgg tttgccggat gcggtgcacc tgtaggccgg
    13201 ataagacgca tcaagcgtcg catcaggcac tatgctcaaa tgccggatgc ggcgtaaacg
    13261 ccttatccgg caatgctatc tcaggctgag gccgaattac tgcgcagctt ccgccttctt
    13321 cgccagacca tccagcagtt tctgatggat cccaccaaaa ccgccgttgc tcatgaccag
    13381 aatatggtcg ccaggctgag cggttttcac caccatatct gccagcgtat ccacatcgcc
    13441 actccagtgt gcaggctgaa cgcaggcttc tgccacttct gccacctgcc acggaatatg
    13501 cgcaggttgc agcaggaaga cttcatcggc acgacctaat gaaggtgcca gatcgtcttt
    13561 gcagatcccc attttcatgg tattcgagcg cggctccagc acagcaataa tgcgcgccgt
    13621 accaccaact ttgccacgca gcgccgcaag cgttgccaga attgccgtcg ggtgatgggc
    13681 aaaatcgtca tataccgtca cgccattcgc ttcaccacgc aactccagac ggcgacgagc
    13741 gttaataaac gaacccagcg cgttagcggc atctgcaggc gctacaccaa catggcgagc
    13801 cgccgcaatc gccatcaggc cattgtgcat attatgttcg cccaccagcg accatttcac
    13861 ttcgcccact ttttcgccat ccagcaagac ttcccattca gaagcgtcag tggtcagctt
    13921 tttcgcctgc cagtgaccct gctcacccac cagctcctgc tcgctccagc agcccatcgc
    13981 catggtctgt ttcaggttga tgtcattttc cggccagata atacggccct gccccggaac
    14041 gatacgcacc agatggtgga actgtttctg gatcgctttc aggtcgtcaa agatatcggc
    14101 gtgatcgaac tcaaggttgt tgaggatcag cgtacgcggg cagtaatgga caaatttaga
    14161 gcgtttgtcg aagaaggcgc agtcatactc atccgcttcg ataacaaaga agtcgctttc
    14221 gcccagacgc gccgaaacct caaagttccc cggcacaccg ccgatcacaa atcccggttt
    14281 gtaaccgcac tgttccagaa tccaggtcgc cattcccgcg gtggtagttt tgccgtgtgt
    14341 accggcaacg gccagcaccc agcgatcgcg cagcacaaaa tcgtgcagcc actgtggacc
    14401 tgacatataa gggatgtttt tttccagtac cgcttccaca cacggatttc cacgggtcat
    14461 ggcgttgcca ataatcacca gatccggctg cggatcgagc tggctggcat cgtaaccctg
    14521 aatcagctca atgccttgct tctcaagtaa ggtgctcatc ggcggataca cattggcgtc
    14581 cgaacccgtt acttcatggc ctaactggcg cgccagcatc gccagaccgc ccataaatgt
    14641 gccacaaatt cctaaaatat gaatgcgcat acgtcactat ccttttttaa tctgccgctc
    14701 attttacgca tatgtcgggc aagtgagaaa cgcatttcag gataatccgc aatttgctgg
    14761 cgcgattcac ctgagcgcaa cattgagatt atttgttaag attgttgcgg tcgctttact
    14821 ccataaacat tgcagggaaa gttttatgaa aacgttaggt gaatttattg tcgaaaagca
    14881 gcacgagttt tctcatgcta ccggtgagct cactgctttg ctgtcggcaa taaaactggg
    14941 cgccaagatt atccatcgcg atatcaacaa agcaggactg gttgatatcc tgggtgccag
    15001 cggtgctgag aacgtgcagg gcgaggttca gcagaaactc gacttgttcg ctaatgaaaa
    15061 actgaaagcc gcactgaaag cacgcgatat cgttgcgggc attgcctctg aagaagaaga
    15121 tgagattgtc gtctttgaag gctgtgaaca cgcaaaatac gtggtgctga tggaccccct
    15181 ggatggctcg tccaacatcg atgttaacgt ctctgtcggt accattttct ccatctaccg
    15241 ccgcgttacg cctgttggca cgccggtaac ggaagaagat ttcctccagc ctggtaacaa
    15301 acaggttgcg gcaggttacg tggtatacgg ctcctctacc atgctggttt acaccaccgg
    15361 atgcggtgtt cacgccttta cttacgatcc ttcgctgggc gttttctgcc tgtgccagga
    15421 acggatgcgc ttcccggaga aaggcaaaac ctactccatc aacgaaggaa actacattaa
    15481 gtttccgaac ggggtgaaga agtacattaa attctgccag gaagaagata aatccaccaa
    15541 ccgcccgtat acctcacgtt atatcggttc actggtcgcg gatttccacc gtaacctgct
    15601 gaaaggcggt atttatctct acccaagcac cgccagccac ccggacggca aactgcgttt
    15661 gctgtatgag tgcaacccga tggcattcct ggcggaacaa gcgggcggta aagcgagcga
    15721 tggcaaagag cgtattctgg atatcatccc ggaaactctg caccagcgcc gttcgttctt
    15781 tgtcggcaac gaccatatgg ttgaagatgt cgaacgcttt atccgtgaat tcccggacgc
    15841 gtaatctcct gcgcctctcc acacggagag gcgtttttat cgctgcgcaa tattgactct
    15901 tgtcactggc gaactctgac gattctccca cagcaccgtt aatccacgct gtaaagcaat
    15961 aaaaatgaac agcaaaatgc cgatggcgat tttcgtccac caggaactca gcgtaccgtc
    16021 aaagttgata taagtctgaa ttagcccctg aatcgccacg ccaaaaagcg tcccaagcac
    16081 cgtcccgacg ccaccactta acaacgtacc gccaatcacc accgacgcga tggcgtccag
    16141 ttccacacct acgcccgcca gtgcatatcc ggcctgggta taaatcgaga agacaatccc
    16201 cgccagtgtt gccagtccgg tggagagcat ataaatgcga atagtggtgc tgcgagtaga
    16261 aatccccatc aggttcgccg acgtcgcgtt gccgccaata gcgtacacct gattgccaaa
    16321 acgggtacga tgcgcaagga aaataccgat aaccaccacc gccagcatca acagccccat
    16381 cgcactaagg cgaccgccgc cggggatttt ccacgcaagg cttgagagcg tgtcataaat
    16441 gggatggttt atcggaatcg actcttccga aacgagatag ctgacgccgc gcaaaaagaa
    16501 catccccgca agcgtaatga taaatgccgg gatcttcagg gcgtcgatca gaagccccat
    16561 aaatgcgccg aaggcacagc ccatcaccag cactagcgga aacgccagca gcggcgagag
    16621 gccgaaatcg ccaaccactt ttgccagaaa cacgccagta aaggcgatca ccgaaccgac
    16681 ggagagatcg ataccgccag agaggatcac aaaggtcatg ccaacggcga ttatcccaag
    16741 aaaggcgtta tcggtcagga tattgcagat cactcttgtg gaagcaaaac cgggaaactg
    16801 ggtcaggcag tagagatagc ccagcacaaa gacgccgatg gtgatcatca gcggcaagtt
    16861 acgttttatc atggctacgt actcctttaa tcagactgat aaagcgttgc gactgaacaa
    16921 tcagcacaca aagcaccacc accgctttta cgacctggtt catctccggc ggaaagccag
    16981 aaagtaaaat tccggtgttc atcccctgaa taatcagcgc ccccacgact gagagcagca
    17041 ggttaaagcg cccgcccatc agcgatccgc cgccaatcac caccgcaaga atggcgtcca
    17101 gctccagcca taacccggcg ttattggcat cggcaccgcg aatatccgcc gccacgataa
    17161 tgcccgcaat cgccgcacat agcccgctca acacgtaggt gagcatgacg atgattcgcg
    17221 tgtttacccc ggcatttttt gccgcccgaa tgttgatacc aacggcttcg ataaacatcc
    17281 ccagtgccgt tttgcgggtc aacagccaga acaggagaag cgtcagtacc gcaataatga
    17341 ctggcgttgg caggaacaac agcgatccac tgccaaacca tgagagatcc ggcgagttaa
    17401 acgtgacgat ctgtccggag gtgatcagtt gcgccacgcc gcgcccggcg accatcagga
    17461 tcagggtggc gacaaatggc tgaattttga ggatcgctac cagtatgccg ttccacaatc
    17521 ccgccaggat gcctgtgccc agggcgctta acaaaacaat cggcaggctg actcccgcga
    17581 ccgtcatcgc agccgttgtg gctccggcga tagccatcac cgcccctacg gagagatcaa
    17641 tcccaccggt ggcgatcacc agcgtcatac caatcgccag taacgcaacg ggtgctgcac
    17701 ggttaagaat gtctatgggg ctaccgaaca aacgcccatc ctgaagcacc acctgccaga
    17761 aatgcggagc taccaggcta tcgaccagca gcaccagcaa tagcgccgcc agctgcggca
    17821 ttcccgttgg ccagcgaaag cgccttttcg gcggcgtagt gtccgggaga gattgaggca
    17881 tcacactgtt ctccttacgc cgcaatggcg ttcatgatcg ccggaacgga aagctctgcc
    17941 agcgggatct ccgccacctg tttgcgatca cgcatgataa tcacccgatc ggcatagccc
    18001 accagctctt ccagttcgga ggagatcacc aacagcgcca gaccgtcagc acacagcgtt
    18061 tcaatcaggc ggatgatctc ggcgtgggca ccaacatcaa tgccgcgggt tggctcgtcg
    18121 aggatcagaa attgcggtcg ggtcagtagc caacgtgaaa gcaacacttt ttgctgattg
    18181 ccgccagaga gaaattcaat cggttgttca gttgaaggtg tgcgaatgcc aagctggcgg
    18241 ataaagcgtt cggcaatctc ttgctgctct ttgcgggaaa tgggtcgtag ccagccgcgc
    18301 tgggcctgga gagcgaggat gatattttcc cgtaccgagg cggcggcgat gatgccatcg
    18361 gttttcctgt cttccgggca gaagccgatg cccagtaccg aagcctgatg tggcgatcgc
    18421 aggttttgct gtttgccttt gatcaacgcc gtgccgctgt cagcaggttt gataccgaag
    18481 atcacttcgg cggtttcggt acgtccggat cccagcaagc ctgccaggcc gacgatctcg
    18541 ccggggcgta cttcgagatc aaacggtgcg atcgttcctt ttttgccgta atttttgaac
    18601 gcggcaacgg gtttgtcgct caacaatgtt cgcccggcac gctgtagcgc gtgggtatcc
    18661 agctcgcgcc ccagcatcat ttttaccagt tcgatctgcg gtagctcgcg cgtttcccga
    18721 cagcctacga aactgccgtt gcgtaagacg gtgatccgat cgctgacctg atagacctga
    18781 tcgagaaagt gagtgacaaa gatcaggctg acgccgcgat cgcgcaactg acgcatcagg
    18841 ccaaacagta actctacttc ctgggtgtcg aggctggcgg tgggttcatc gaggatcagc
    18901 actttggcgg agagatcgat agcccggcaa atggcgacga tttgctgcat cgccaccgaa
    18961 aaacggttaa gcggttcacg cacgtcgagg gagaaaccgt aagatgtcat cagttcggtg
    19021 gcacgctttt ccatctcttt gcggcgtaga aagccgaaac gtttgggttc gcgacctatg
    19081 aatagattat cagcgaccga catattgggt agcaggttga cttcctgata aacggttccg
    19141 atgccgagtt gttgtgcatg ggcggtattt ttcggtgaga tagcctggcc ttccagccag
    19201 atggtgccgc gatcggcgtg gtatacaccg gttaatgctt tgattagcgt cgattttccc
    19261 gccccgtttt caccgagcag cgccatgatt tcgccacggc gcaggctgaa atcaacgttg
    19321 tctaacgctt tgacgccggg gaaaaattta cttaatcctt cggtgcggag gatctcctgg
    19381 tgttggttag tcgtcatggt ttccccctta gccacgctct aaaagtctgg gaatagatcg
    19441 tgcctgatgt taagagctgc gccgtttgtc cccctcaccc taaccctctc cccgaagggg
    19501 cgaggggaca gattgtgcac ggtgttaagt ccggtgttgc accactgccg gatgcggcgt
    19561 gaacgcctta tccggcctac acagagcacc gaactcgtag gcatgataag acgcggtaag
    19621 cgtcgcatca ggcttcagcg caccaccgcc ggatgcggcg cgagcgcctt atccggccta
    19681 cgaatcggca tcaggcatag caaccctcaa taccccatat ttttcttctt ctctaactct
    19741 tcttttgcgg tatcaggcag ataaagggtg gatttggtca gcgtcagctt ttcaggcatg
    19801 gtgccgtctt ttttgtattt ctccagcgcg tcgaaggcgg gacctgccat attcggcgtc
    19861 agttcaacac tggcgttcgc ttcgccatca atcatcgctt tgtagatatc gggtacaccg
    19921 tcaatggaac ccgtgaggat atctttgccc ggtttcaggc ccgcttcttt aattgcctga
    19981 attgcgccaa tcaccatgtc atcgttatgg gcgtaaacca tgcagatgtt tttgccgttg
    20041 ttttccgctt tgataaagct ctccataact tctttgcctt tactgcgggt gaagtcacct
    20101 gactgcgagc ggatgatttt gatatttggc gcattcttaa tggcttcggc aaagcctttc
    20161 ttacggtcaa tggcgacgct ggccccaacg gtgccctgca gctccaccac gttgcatggt
    20221 ttgccattca cttcttttac cagccagtca ccgatcaact taccttcgag gatgttgtcg
    20281 gcagtaacgg tggtcatata gagagatttg tctttcacat cgatggaacg gtcgagcaag
    20341 aagaccggga tttcggcatc tttcgcctct tttaataccg gttcccaacc tgtcgcgacc
    20401 accggagcga taaagatcgc atccacccct tgtgcaacga aggaacgtac cgctttaatc
    20461 tggttttcct gcttttgctg accatcagca attttcaacg taattccgcg tttttcggcc
    20521 tcacttttcg ctacattggt ttctgcagcg cgccagccgg attccgatcc gacctgcgaa
    20581 aatcctacgg ttaatggagc ggccaacgcc atagacgaca tggctgccga gactgcagag
    20641 actacaagta agcgtttcca catagggttg tcctcgtcgg ggtaattatt attggtgaaa
    20701 agatgttcgc gaaaaaacta tagacaattc gttatgtaac ggattgcgtt acatcacact
    20761 tcaaaaaagt aaataaaacg ttaatcacaa gtttgtaatc gctttcatct cactatgaaa
    20821 aatgcggcta cggttatgga ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag
    20881 gaaaatgaag acgaaaacaa gcaaagacat tcggcgcgag ttggctataa tactcggcac
    20941 ttgtttgcca catattttta aaggaaacag acatgagctt actcaacgtc cctgcgggta
    21001 aagatctgcc ggaagacatc tacgttgtta ttgagatccc ggctaacgca gatccgatca
    21061 aatacgaaat cgacaaagag agcggcgcac tgttcgttga ccgcttcatg tccaccgcga
    21121 tgttctatcc gtgcaactac ggttacatca accacaccct gtctctggac ggtgacccgg
    21181 ttgacgtact ggtcccgact ccgtacccgc tgcagccggg ttctgtgatc cgttgccgtc
    21241 cggttggcgt tctgaaaatg accgacgaag ccggtgaaga tgcgaaactg attgctgttc
    21301 cgcacacaaa gctgagcaaa gaatacgatc acattaaaga cgttaacgat ctgccagagc
    21361 tgctgaaagc gcagatcgct cacttcttcg agcactacaa agacctcgaa aaaggcaagt
    21421 gggtgaaagt tgaaggttgg gaaaacgcag aagccgctaa agctgaaatc gttgcttcct
    21481 tcgagcgcgc aaagaataaa taagttcttc tagcgcaata accctgaacg ccgggcttcg
    21541 gttagtaagg gtttttttat gcccgcgata aataaactct ctattccacc atcattattc
    21601 tcagcggttg caaggcttga acggtaagaa caagcaaacc cgaccaccat tttgctgttc
    21661 atagccactt gctggaagtt agccgacctc actcatactc accgttcaaa gcgtgccccc
    21721 gttttacccc tgcacatttg tgaacgccag cagagttttt tatgcgattg cagatatgga
    21781 aagcggctcg gagagaaaag caaaagatgg aaaagttaca aaaaaaaatc gcgagataca
    21841 tcgcataaaa caacgccacc cgaaggtggc gtgcttttat tattacttat ccctgtctaa
    21901 ccagtcgccg ctttcaatta gctttaatcc atcgacgggt cgttgataaa cgtacatcca
    21961 tgcactcccg tacggcgtct gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa
    22021 ggcatcaagt tcggccagcg tggcgttgtc aatacgataa acttcaccgt gtactgttcc
    22081 gttccccgga actgcgcctg gatagtggcc caggctatac aactggtagt tatcgatact
    22141 gaaatcgccc agtaactggg cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa
    22201 actgccgtag acaaatattc gcattgctaa aactcgaact gatagagcaa atccagtgcc
    22261 tggtctacac cagacacggc ttccagatat agcttaggca tcaggcgata acgtaacgtg
    22321 agtgttgcta tagagtcaaa tatacccacg ccgtatttca cttgcagacc tggcaataca
    22381 tagccgctga ccactacctg ggaggagtcg cctactccct gagtgtcgag cgctaaattg
    22441 cttacgccaa acgtctcgcc gattttaccc acaatctggc cactttgcgc aacccccagg
    22501 ccaatcagca ttgaggtcat cgccgcactg tcgctctgat cgctctccag cccttgtcca
    22561 cgtagcaaat aagacaatgc agcttgttgc gacatagccg ggtcagagaa gatctccgct
    22621 ttcggttcgt ccgccagacc agtgacgcga actccggcga ttacgtcgtc ttctgtagca
    22681 tccgggttac gaatagcttc aatattaaga tacggttgat ctggcggacc agagaacagt
    22741 agctcaccct tacgcacaat cagatcctga ccataggcat ggaagcgccc ttcagggatg
    22801 ttgatctgcc cgttcaggcc cagcccttgt ttgtcctgaa cgacgttaag atcgcccgtc
    22861 agccgcgctt tcaggccaaa ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg
    22921 ttactgttaa tcggaatcga cgccgttttc ggctcttccg gttgcaggtt atcgttaagc
    22981 atcaccacat cgctggagac gcctactgcg ctttccggca gatcgtgcac cacgatgcgc
    23041 gcccacggga catccacgcg accatcgagg gtaaacaggt ttggtgtagc ctcgaataca
    23101 acatctggcg atacatccat tcgtaccatc ggcggcacgg tgatccgcac tttactgcct
    23161 ttcgccgtta ctcgtgcccg ccagttttca atttggctcc agtcggcatc accgttcagg
    23221 tagatttcac cctgctgggt ccgtacggta ccggcaagcg tcgagcgcat accgttaaag
    23281 ttgaccgcaa gctggctcgg ctgcatatca aacggcataa agttgccgtc gatatccaca
    23341 cccgtaacct gaagctgacc aaacaactgc gggctttgca catcaccacc cagacgcaag
    23401 ttggcactca ccatccccgc tgctttttcc ccacgggtaa agatggggtt tatcatcgca
    23461 aggttgaagt tacggatatt gacgttgcca ccaagattac ggcggccttg cggatcggtc
    23521 acctgcacct gtccatcaaa ctggccgtta ttggtcaggc ggatggtcca gcccaattcg
    23581 gcacggttgt tacgcaattc cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc
    23641 gcatcgttga cggtttgcgt tacctgcacg ttacgccccg aaagggtgat actgccctgc
    23701 ggcagcccct ctttcgtggt gtcccaggca acatccgctt tacccgtgaa gataccgctg
    23761 gcctgagtgg tttctggcat aaacggtttc agcatggcga ggtcgaagcg gttgagattc
    23821 accaccgcac gcccttcggc ccccgcatcg atagtttgcg gcacgcacag ttccgcattc
    23881 gggttaagcc agcagtgtgg cccgatgctg attttttgct ccttattgcg gtaatccagc
    23941 gcaatatcgc gggtcagcga ccacgggccg accggcgtct ggaagcgggt attgctaaga
    24001 gttcccttcc agcgttcttc tttgcgatca aaacttcctg ccagattaag ctgcccggag
    24061 acaggctcgc cctgaatccg caactgtagc tcgtgctgct tttcgctgcc tttggcattc
    24121 agggtgacga ggttgatatt tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt
    24181 ttcccggcga tctgatcggt ggatttaatg tcgccttcca cgcgaacctg cgccacggaa
    24241 agttcctgcc agcgcaggcc gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc
    24301 gtgccgcgta ctttcaccag ccctttcgct gtaccgccaa gccccggcag cgcgttatcc
    24361 agccccggcg cgttgatggt ggcatcaaga ttgagatctt ttacccccag ctcgcctttc
    24421 acttcggcac tgtttggccc gagttccaga tgaagcccag ggatcatcca ctgcatataa
    24481 ctgttgcctt tcagcgtacc gtcaacgttc actttgttct gtttgacgtt accggtcagc
    24541 ttcagttctg gcacctccat ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc
    24601 aagccattga gtttcgacgg ccactccggg aactctttgg cggtgttaat gccattaagc
    24661 gttagctcac cgcgccaact aatggcctgc tgccagtcga gcaacgcctt gagttcagtt
    24721 ttcccttcca gcgccgcgac ggtgagtttg tcgagattga cctgctgttc attacctttg
    24781 gcatcaaggg taatggtggc tggcgggatc tcctgtccct tcactgccgt acgcatagag
    24841 agcgtgtaat cggtcatttt gccggtaagt ttcagtttca gatcatccgc ctgatactgc
    24901 ttctcaccag tgaacggcca gtaaagctgt ttgctgttca cttccacgtt gagcggcaat
    24961 ccggcttccg ccagtcgcgt ctgggcgcgt aaatccatat ccaccggacc ggaaagatta
    25021 acgccaatct ccagctgttc gcgcagcgcg ccacccactt ttagcttcac tttttcacct
    25081 ttcaacggct ccacgttcag tgtgctgttg agggtgatat ccaccggcca gttgtctgac
    25141 agctgcgccg tgccgctggc gttgacgatc ccctggttgg aatcgatatc cagggcgtcc
    25201 agtttagtat tgccgtcaat gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc
    25261 gtgtcgcccg tcacgcgtaa ctgctcgcct ttaaactctt caatgttcag gttaagcggc
    25321 aaatgcaagt cggtcatttc cggcaatacc gggcgagaaa agagatcttt cagcgtttcg
    25381 ccgagcggct tttcctccgg ctgcggattt tcaattttcg gttcgaccac ttcttcctgc
    25441 gccacttccg ccactttcgg cagagcaatc agcaggcctt tcagcgacgt cggtttcagg
    25501 gtcagggttt tctcctgcca gttcaggccg gaggtgaagt ccatcaccga caccgtggtg
    25561 tcatcaatct tgatgttgac gttgtccagc gccacccgtg tcagggtgat gggatacggc
    25621 gtggagagat ccagcggacc gctatcttcc tcttcttcaa cctgttcaga aggaggcatt
    25681 tttttactgt cgatgttgac ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta
    25741 ctgttccaca ggcactcaag accgaccgcc agatgcagat tacccgcttt taccgccacg
    25801 cctggctgct cataacgaac gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg
    25861 ccaatatcca gtcctggcac ccagcgatcc gccgctttaa ataccagatg caggccgctg
    25921 gtggtgccca ccagaaacgc caccgatccc agcaacagta agataacgat aaccacgccg
    25981 aggctgattt ttttccataa actcataatt ctggccccag accgatgtaa aactgtaacc
    26041 cgtgctcgtc tttatccgcg accggtacgg caaaatcgag tttgattggc ccgaccggcg
    26101 attcccagcg tacgccgacc ccggtaccgg ttttaaagtc gctgcggcga atatcgctta
    26161 ccgcttcgcc actatcgaca aacaccgcgc cccaccattt tccggtcacg ttgtactggt
    26221 attccagcga tccggttatc agcttcgagg cccctttcag gtcaccgttg gagtatttcg
    26281 gggcgataga tttgtatttg tagccgcgaa tactgcgatc gcccccggcg aagaaacgca
    26341 gatccggcgg tactttgtcg aaatcaccgg tttcaatcca gcccagcgtg ccgcgtgtca
    26401 caaaacgatg gcgatcatac agtgtgcgga tccagacgtt ttgtgcctgg aaaacggaga
    26461 aatcgacatc tgagccccag gccgtgttgg agtagtcgat agagtagcgt tgcgagtcgc
    26521 cccaggttgg catcaggcca ccacgagaac gcgtgcggct aatcatcacc ccaggataaa
    26581 acagcatcgt ggtgttggta atttcaccct gagtaaagtg gtcgagactc cagcgcaggt
    26641 taatggcacg ctgccagccg ctggagagat cccagtagcg agaagccacc agcgtagtgg
    26701 agtcagattc ggtatcgttc aggtcagtgc gcttaaaacc gccctgcacc aaataatatt
    26761 gttccagtgg attcttcagc agtggcattt tatagctgaa gtcgagggtc tgttccggcg
    26821 cggaaatact ggtactggtg gtcaaactgt gaccgtatga gttcatccac ggctttttcc
    26881 acgtcgcttt cacgcgcggt cccacgtccg tagagtaacc gaccccggtt tcgatggtgt
    26941 tttctgttcg cggcgaaacc acgcccgtca atggtaatac tttcgtttcg cgcgctttat
    27001 caaattgtgg agccaccacc accgagttaa accagccggt agcagaaagt cggcggttca
    27061 gttccgccag atctttcgat tcatactcat cgccctcttt aaacggcacc agattttgca
    27121 ggtattcatc gcggatttgt gatccttcaa aggtcacatg cccaaagcgg taacgttcgc
    27181 cactgttata atcaatatcc cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg
    27241 ctttggtaaa ttcgctatcg aaataacctt tacgcaacgc aatactggtt aaggactttt
    27301 tgaaattttc ataatcgccc tggttcagta ccgtgccaat agccgggcga gtatcgagca
    27361 atttcaaata gtctttatcg gtccgcgcgc cgccgcgcaa taccacatca gtgccgccaa
    27421 ttaacaccgg cacgcctggc gtgactttgg cgatcaatac ctgccgccct ttctttggcg
    27481 gtggacggag atcaaattca atggtcggct ggtaataacc cagcgctttc agaccttcgc
    27541 ggatggcatc atcgacgcgt gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa
    27601 tcgtagaaag ctgcgcacga acgttctttt ccagctgtcc cgataacccc tcgacctgta
    27661 gacggacgtt cgcggcgacg gcagatccgc ttaagcagag taagcttaca cagcataact
    27721 gtcggatata gcgcacattt tctcctgaat atcctttttt cctgcccctg gaacgccgtt
    27781 aaacggctta acaaaaatcc agtaatatgg attaaaaagc agactaaacc ccaaatattt
    27841 cttatgtttt actttagacc tattcacggt ggttattgtg tgcaaatacg cctcttgtta
    27901 caaccttaac cccaatgacc gattttcggg agagcgacac catgagttta tttgataaaa
    27961 agcatctggt ttcccccgcc gatgccctgc ctggacgtaa caccccgatg cccgtagcca
    28021 cgctgcatgc ggtcaacggt cactcaatga ccaatgtacc tgacggaatg gagattgcca
    28081 tttttgcgat gggttgtttc tggggtgtgg agcgtctgtt ctggcagtta cccggcgttt
    28141 acagcaccgc cgcaggctat accggcggct atacgccaaa tccgacttat cgggaagtgt
    28201 gctccggtga tacgggtcat gccgaagcgg tacgcattgt ttacgatcct tctgtcatca
    28261 gctatgagca gttgctacag gtattttggg agaatcacga tcccgcccag ggaatgcgtc
    28321 agggcaatga ccacggcacg cagtatcgtt cagcgattta tccgctgacc ccagaacagg
    28381 atgccgcagc tcgcgccagt ctggaacgtt ttcaggcggc gatgcttgcc gccgatgacg
    28441 atcgtcacat caccacggaa atcgctaacg ccacaccgtt ttattatgcc gaagatgacc
    28501 accagcaata tctgcataaa aacccgtatg gttactgtgg aattggcgga attggcgtct
    28561 gtctgccacc ggaagcatag cgttacgggt acaaatgtag attgttgata aagtgcgttt
    28621 tgtttatgcc gaatgcggcg tgaatgcctt accaggccta caaaatcgtc caaattcaat
    28681 atattgcagg gactgcgtag gcctgatcgg catagcgcat caggcagtat tgcatttatc
    28741 agcagtctga attttaaccc tctggcgact ttacagtacc ttacgctata ctagccactg
    28801 aaaatgccgg ttcactttct tcgaatcggc tttcaatgtg tatttcacac aaattaatca
    28861 acttcccctt ccgaggatct ggcctgaaag gtcggataag atatgttaaa cagtatttta
    28921 gtcatactct gcttgatcgc tgtaagtgcg ttcttctcga tgtctgagat ctcacttgcc
    28981 gcctcacgca aaatcaaact taaactgctg gctgatgaag gcaatataaa tgcccaacgc
    29041 gttctgaata tgcaggaaaa tcccggcatg ttctttacgg tggtccaaat cggtctgaac
    29101 gcagttgcga ttctcggcgg tatcgtcggt gatgcggcat tttctccagc ttttcacagc
    29161 ctgttctccc gctatatgtc ggcagaactc tctgagcaac tgagctttat tctctctttc
    29221 tcgttagtga ctggcatgtt tatcctgttt gcggatttaa ccccgaaacg catcggtatg
    29281 attgcgccag aagcggtggc tttgcgtatc atcaacccga tgcgcttctg cctgtacgtt
    29341 tgcaccccgc tggtgtggtt cttcaacggc ctggcgaaca tgatcttccg tattttcaaa
    29401 ctgccaatgg tacgtaaaga tgacatcact tctgatgaca tctacgcggt agtggaagcc
    29461 ggtgcgctgg cgggcgtgtt acgtaaacag gaacacgagc tgattgaaaa cgtctttgag
    29521 ctggaatccc gtaccgttcc gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat
    29581 ctccacgaag atgagcaaag cctgaagaat aaggtggcgg aacatccgca ctctaagttt
    29641 ctcgtctgta atgaagatat tgaccacatc atcggatatg tcgattctaa agacctgctg
    29701 aaccgcgtgc tggctaacca aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg
    29761 ctgattgtgc cggatacgtt aaccctttca gaggcgttgg aaagttttaa aaccgcaggt
    29821 gaagacttcg cggtgatcat gaacgagtac gcgctggtgg tggggatcat caccctcaac
    29881 gacgtgatga ccacgctgat gggcgatctg gtcggtcagg ggctggaaga gcagattgtc
    29941 gcccgtgatg agaactcatg gctgattgac ggcggcaccc caattgacga cgtcatgcgc
    30001 gtgctggata ttgacgagtt cccgcagtcg ggcaactacg aaaccatcgg cggctttatg
    30061 atgtttatgc tgcgtaagat cccgaaacgc accgattcgg tgaaattcgc cggctacaaa
    30121 tttgaagtgg tggatatcga taactaccgc atcgaccagc tgctggtgac ccggatcgac
    30181 agcaaggcca ccgccctttc gccaaaactg cctgacgcta aagataaaga agaaagcgtc
    30241 gcgtaacccc cagaaacatc aacggctcct gaatcaggag ccgtcttatt actgcatagc
    30301 actttggtta agccatctct gtttgcagac gcataacctg acggttaact tcggacatca
    30361 ctgacaaatg cagcttatcc ttcacttttg ggataagaat cttaccctta tcaaattcaa
    30421 aagcgccaac gtccttgatg tataaccgtc cacggaacag gatctttacg tacttcgcca
    30481 cctgaagtgg gttgtaccgt tggaaaattt tcattgttat ctcctgctga gtattacgcc
    30541 tgtgcggtgc cacaatcggc ccaactatta tgaggcgcaa attttaatgc ctagtgacta
    30601 tagactatcc gggcaatgtt tccaccgcgt ataacttttt ttacctaaag gttacaatta
    30661 ttcagaatta tctttttacc gaagcgcggt cttcagtata agcattcatt tttcatatga
    30721 tttgtgcgct tgaccgcaaa ctggcatcac acttgcggga aattcgataa atagcacata
    30781 tgattaaaac tcagacccaa gtggtcggat cacctgcata tcataagaag gaaacaccat
    30841 gaccctacgc aagattctgg cactcacctg cctgctgttg ccgatgatgg cttccgcaca
    30901 tcagttcgaa accggtcagc gagtgccgcc gattggcatc accgatcgtg gcgagttggt
    30961 gcttgataaa gatcagttta gctacaaaac ctggaacagc gcgcagttag tgggaaaagt
    31021 gcgagtactg caacatattg ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat
    31081 tgaagcgatt aaatcagcga agttaccgca cgatcgttac cagaccacca ccattgttaa
    31141 caccgacgac gcaattccgg gttccggcat gtttgtgcgc agcagtctgg agagtaataa
    31201 aaagctttat ccctggtcgc agtttattgt tgatagcaat ggcgtcgcac gcggtgcctg
    31261 gcagctggat gaaaaaagtt ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg
    31321 ggccaaagac ggggcgctta ctcaggaaga ggtgcagcaa gtgatggacc tgctgcataa
    31381 attaattaat aaatagacac tctgaaccca ggattcagga acgactcgcg cggagtgtaa
    31441 tccagcggtt taccctgcca gtcgtgaacg tgcgctccgg cagctgcggc cacagcatgt
    31501 ccagcggcgg tgtcccaaat attcgttggt ccgaagcgcg ggtacagctg cgcctgtcct
    31561 tccgccacca ggcagaattt cagcgaagag ccgatggacg tggtctgatg ttcgccaagc
    31621 tgttgcagat actctttcag ctccgcatct gcatgggaac ggctgataac caccagcggc
    31681 gggcgcgcat cgcggacctg aatctgcttg cgcacaccgc actcttcttt ccaggccttg
    31741 ccttctgccg cgctgtacat tacgttcatt accggcgcat acaccacacc taaaatcggt
    31801 ttgccatggt caatgagcgc aatgttaacg gtgaattcgc cattgcgttt aataaactct
    31861 ttagtaccat ccagcgggtc taccagccag taacgctgcc agtgctgacg gacttcccaa
    31921 ccgggaggat cttcttcaga aaggaccgga atatccggtg tcagcgtacg taaaccgtcc
    31981 atgataacgg tgtgtgcggc aatatccgct gccgttaccg gagaattgtc cgctttgctg
    32041 acgacgtcca tcggtttcgt cccgtcgtag acctgcataa tggcatcgcc tgcattccgt
    32101 gcaagctggc atacttgatc taacatttct ccacctcgtc tctgtgagcg gtgttaactt
    32161 attgttttac ttatacccta tcgttaatga atgcgccaac tgtgatagtg tcatcatttt
    32221 caaagcgtaa aattgtggca ttcttcactg ttctataagt aagacgttta ttctcccttt
    32281 tctttcgtat tcccgatgat aaaaggatgt ccctgatgat taagtttagc gcaacgctcc
    32341 tggccacgct gattgccgcc agtgtgaatg cagcgacggt cgatctgcgt atcatggaaa
    32401 ccactgatct gcatagcaac atgatggatt tcgattatta caaagacacc gccacggaaa
    32461 aattcggact ggtacgtacg gcaagcctga ttaacgatgc ccgcaatgaa gtgaaaaaca
    32521 gcgtactggt cgataacggc gatttgattc aggggagtcc gctggcagat tacatgtcgg
    32581 cgaaaggatt aaaagcaggt gatattcacc cagtgtataa ggcattaaat acgctggact
    32641 ataccgtcgg cacgcttggc aaccatgagt ttaactacgg tctggattac ctgaaaaatg
    32701 cgctggcggg agcgaaattc ccttatgtaa atgccaacgt cattgacgcc agaaccaaac
    32761 agccaatgtt tacaccgtat ttaattaaag acaccgaagt ggtcgataaa gacggaaaaa
    32821 aacagacgct gaagattggc tatattggcg tcgtaccgcc gcaaatcatg ggctgggata
    32881 aagctaattt atccgggaaa gtgacggtga atgatattac cgaaaccgtg cgcaaatacg
    32941 tgcctgaaat gcgcgagaaa ggtgccgatc tcgttgtcgt tctggcgcat tccgggctgt
    33001 ctgccgatcc gtataaagtg atggcggaaa actcagttta ttatctcagt gaaattccgg
    33061 gtgttgacgc cattatgttt ggccatgctc acgccgtttt cccaagtaaa gattttgctg
    33121 atatcgaagg agctgatatc gcgaaaggca cgctgaatgg tgttccggcg gtaatgccag
    33181 gcatgtgggg cgatcatctt ggtgtggtcg acttacaact cagtaatgac agcggtaaat
    33241 ggcaggtgac gcaggcgaaa gcggaagctc ggccgattta cgacatcgcc aataaaaaat
    33301 ccctcgcggc ggaagacagc aagctggtag aaacactcaa agccgatcac gatgccacac
    33361 gccagttcgt cagcaagcca atcggtaaat ctgccgacaa tatgtatagc tatctggcgc
    33421 tggtgcagga cgatccgacc gtgcaagtag tgaacaacgc gcaaaaagcg tatgtcgaac
    33481 attacattca gggcgatccg gatctggcaa aactgccggt gctttcagct gccgcaccgt
    33541 ttaaagttgg tggtcgcaaa aatgacccgg caagctatgt ggaggtggaa aaaggccagt
    33601 tgaccttccg taatgccgcc gatctttatc tctaccccaa tacactgatt gtggtgaaag
    33661 ccagcggtaa agaggtgaaa gagtggctgg agtgctccgc cggacagttt aaccagattg
    33721 atcctaacag cacgaaacca cagtcactca tcaactggga tggtttccgc acttataact
    33781 ttgatgttat tgatggtgtg aattatcaga ttgatgttac ccaacccgcc cgttatgacg
    33841 gcgagtgcca gatgattaat gccaatgcag agcggattaa gaacctgacc tttaatggca
    33901 agccgattga tccgaacgct atgttcctgg ttgccaccaa taactatcgc gcttacggtg
    33961 gcaaatttgc cggtacgggc gacagccata tcgcttttgc ttcaccggat gagaaccgct
    34021 cggtgctggc agcgtggatt gctgatgagt cgaagcgtgc gggggaaatt cacccggcgg
    34081 cagataacaa ctggcgttta gcaccgattg ctggcgataa gaaactggat atccgtttcg
    34141 aaacttcccc gtcagataaa gccgcagcgt ttattaaaga gaaagggcaa tatccgatga
    34201 ataaagtcgc gaccgatgat atcgggtttg cgatttatca ggtggatttg agtaagtaaa
    34261 acacttcttt ttcggcctat aaatcatcaa ccgcatccgg catttattgg cggatgcgat
    34321 gctggcgcat cttatccgcc ctacaagcca tgcaccgtag accagataag ctcagcgcat
    34381 ccggcagtta tgccgcacgt tcatcccgca ccgccaacac ctcgggcaaa ttcaactcaa
    34441 tccagtccgc cagtgcagca accttttcgc tcacctgctc gcccagcggc gtgaggctat
    34501 attccacatg cggcggcacc accggatacg cgatacggtt aagaaaacca tcctgttcta
    34561 acgcctgtaa cgactgcgca agcatctttt cgctcacccc gccgattttg cgccgcaggt
    34621 cgctaaagcg atgagtacct tcgcgtagcg ccaccagaat caacaccccc caacggctgg
    34681 tgacgtgttt caacacctcg cgcgacgggc actgttccgc aaagagatta ccctctttca
    34741 gttgttgcga caggctaacc tgactcattt catacttacc tttttgtgcg tacttactaa
    34801 aagtaagttt aggtgttagc gtatttaaac acaagacaaa acgatggaga cttcccatga
    34861 tcgctattac tggtgccact ggccaacttg gtcactatgt tattgaatcc ttgaagaaaa
    34921 cggttcctgt cagccaaata gtggctatcg ttcgtaatcc ggcaaaagcc caggctctgg
    34981 cagcacaagg cattaccgtg cgtcaggctg actacggcga tgaagccgca ctgacatctg
    35041 cacttcaggg agtggaaaaa ctactgctga tctcttccag cgaagtgggt caacgtgccc
    35101 cgcagcatcg taatgttatt aatgccgcaa aggcggctgg tgtgaaattt atcgcttata
    35161 ccagcctgct acatgcagat acctccccgc tcggcctcgc cgatgagcac atcgagacgg
    35221 agaaaatgtt ggctgattct ggcatcgttt acaccctgct gcgcaacggc tggtacagcg
    35281 aaaactacct cgccagcgcc ccggcagcac tggaacacgg cgtatttatc ggtgcggcgg
    35341 gcgatggcaa aatcgcctca gcaacgcggg cagattatgc ggcagctgcg gcacgcgtga
    35401 ttagcgaagc cggtcacgaa ggcaaggttt acgaactggc gggcgatagt gcctggacgt
    35461 tgacacagtt agcggcagag ctgaccaaac agagcggcaa acaggttacc tatcaaaatc
    35521 tgagcgaagc cgatttcgcc gcggcactga aaagcgtcgg actgcccgac ggactggcgg
    35581 atatgctggc ggattctgac gttggcgcat cgaaaggcgg tctgtttgat gacagcaaaa
    35641 cgcttagcaa attgattggc cgcccaacga caacgttagc cgaaagcgta agccatcttt
    35701 ttaatgttaa taactagtta attaaagtgg catcctcccg catcctctct gataatgacg
    35761 ggatgccggg agcaatcatg tctgcttcct gaacgtttct tctgacagac caatggatgc
    35821 cagtaatgat tagcggcgtg ctgtacgccc tgttagcagg gttgatgtgg gggcttattt
    35881 ttgtcgggcc gttgatcgtg ccggaatacc cggcgatgtt gcagtcgatg gggcgttatc
    35941 tggcgttagg gttaattgcg ctacccattg cctggctggg acgcgtgcgt ctgcgtcagt
    36001 tggcgcgtcg ggactggctt accgccttga tgctcactat gatgggcaac ctcatttatt
    36061 acttctgcct tgccagtgcc attcaacgta ctggcgcgcc tgtttccacg atgattatcg
    36121 gcaccctgcc ggtggtgatt cccgtttttg ccaatctgct ttatagccag cgcgacggca
    36181 aactcgcatg gggaaaactc gccccggcac tgatttgtat tggcatcggc ctggcgtgtg
    36241 tgaatattgc tgagttaaac cacggactcc ccgattttga ctgggcacgt tatacctctg
    36301 gcatcgtgct ggcgttagtt tccgtggttt gctgggcatg gtatgccctg cgcaacgccc
    36361 gctggctgcg ggaaaatccc gacaaacatc cgatgatgtg ggcgacggcg caggcgctgg
    36421 tcacgctgcc ggtttctctc atcggctatc tcgtcgcctg ttactggctg aatatacaaa
    36481 cgccggactt ctccttacct tttggccccc gtccgctggt gtttattagt ctgatgattg
    36541 cgatagccgt gctttgctca tgggttggcg cactctgctg gaacgtcgcc agccagcgat
    36601 taccgacagt gattctcggg ccgctgattg ttttcgaaac gctggcaggt ttgctgtaca
    36661 cctttttact ccgccagcaa atgccgccgc taatgacgct gagcggtatc gcgctgttag
    36721 tgattggcgt ggtcatcgcg gtcagagcaa aaccagaaaa gcctttaact gaatctgtct
    36781 cagaaagttg acacgctggc agtgagttaa ataagcctct gctacgtaag ggttatagct
    36841 tttgccttaa agatgcattt aaaatacatc ttatcttttt aagaatgagg tatcagctat
    36901 ggcttatcgc gaccaacctt taggtgaact ggcgctctct attcctcgcg cttcagctct
    36961 gtttcgtaaa tatgatatgg attactgctg tggcggtaag caaacgctgg cgcgcgcggc
    37021 ggcacgtaaa gaactggatg ttgaggtcat tgaagctgaa ctggcaaagc ttgctgaaca
    37081 accgattgag aaagactggc gtagcgcccc gctggcagaa atcattgacc atatcatcgt
    37141 gcgctaccac gatcgtcacc gcgagcaact gccggagctg atcctgcaag cgactaaagt
    37201 cgagcgcgtt cacgccgaca aaccgagcgt gccaaaaggg ctgacaaaat acctgactat
    37261 gctgcatgaa gagctttcca gccacatgat gaaagaagag cagatcctct tcccgatgat
    37321 caaacaaggc atgggcagcc aggcaatggg gccaatcagc gtaatggaaa gcgagcacga
    37381 tgaagcgggc gaactgctgg aagtgattaa acacactacc aataacgtca caccgccgcc
    37441 agaagcctgc accacctgga aagcgatgta taacggcatt aatgaactga ttgatgacct
    37501 gatggatcac atcagtctgg aaaacaatgt actgttccca cgcgccctag cgggtgagtg
    37561 atctaaaagc tggatggcat tgcgccatcc agcatgataa tgcggttatt tccgcagttc
    37621 agcagcccgc ttcttaccaa taaacagcca gcccaacccc agcgcgataa accacagcgg
    37681 ggtaaccagc agcgcctggc gagtgtcatc ttccagtgtc agcagcacga tcacgaacac
    37741 aaagaacgcc atacataccc agcacatcag cttgccgagc ggcatcttgt agatcgactt
    37801 ctcatgcaga tgaggacgct gtttgcgata caccaggtac gagcaaagga taatcgtcca
    37861 gacgaacata aacagaatcg cggaaaccgt cgtgatcatc gtgaacgcgc caatcacgct
    37921 gggattcaca tacagcatca ccacgccgcc gagcagacag atgcaggaga aggtcagccc
    37981 tttcgcgggt actgcgcgct tagaaagttt agcgaacgct ttcggtgcta caccttcctg
    38041 cgccagacca aacagcatac ggctggtaga gaagacgccg ctgttagcgg aagacgccgc
    38101 agaggtcagc accacaaagt tgatcacgct tgcggcagca ggcagcccta ccaacacgaa
    38161 cagttcaaca aacgggcttt tctccgggac taccgaactc cacggcgtca cggacataat
    38221 tacaatcagc gcgaagacgt agaacataat gatacggatc ggaatagagt taattgcacg
    38281 cggcagtgat ttctccggat ctttggtttc cgcagcggtt gtacctacca gctcaatccc
    38341 tacgaaagcg aaaaccgcta tctggaatcc ggcaaagaag ccacttaaac ctttcgggaa
    38401 ccagccgcca tcattccaca aatgcgcgaa tgacgcttcc acaccagtcg gtgactgaaa
    38461 gtgcatcgcc accataacca ggccgacgac aatcagcgac acaatggcga cgattttgat
    38521 catcgcaaac cagaactcca tctcaccgaa cattttcacg gtggcgagat tgagcgtcag
    38581 cagcagcact atcaccgcca gcgaggcgac ccagtcggag agatcgggga accagaactg
    38641 agcataagca gtgatcgcca ccacgtctgc cataccggtt acaacccagc agaaccagta
    38701 agtccagccg gtgaaatatc ctgcccacgg cccgagtaaa tcggaagcga agtcactaaa
    38761 agatttgtat tccagattcg aaagcagcaa ttcccccatt gcccgcatca cgaaaaagag
    38821 cataaaacca atgatcatat aaacgaaaat gatcgacggc ccggcaaggc taatcgtttt
    38881 gccagacccc ataaacaacc ccgtaccaat ggcaccgcca atggcaataa gctgaatatg
    38941 tcggtttgtg agattgcgcc gtagcgactg ttcagccgga gcctgatcat cggcaacgac
    39001 ttttacctga tctaccatgt ttttttcttc ctgtacctgt ctgtgttgtt caggctctac
    39061 ggcctttagt cagtctatga tacgacgaat ctgtatcggg actcatcgat attaggtaac
    39121 aatacgcggg atgaatacta agatttagat ctaatgttaa ttttatgttt aaagtgagtg
    39181 tcatatcacg gtgataacgc gaaacagctc acaaaaatac acgcttacgg ggtctttgca
    39241 acacaaaata gcgccgtttg gcaaactgaa gggtttattg ctgaatgcct gctcccctct
    39301 ggttcgaggg gaacagaatg cgctgcttag aggatttcca gcagttcgac ttcaaacacc
    39361 agggtgctga acggagggat ggatgcgcct gcgccgcgct cgccatatgc cagttcctgc
    39421 gggatagtca gttcccattt agaacctacc ggcatcagag tcagtgcttc aatccagcca
    39481 gggatcacgc cattaaccgg aaattcagcg ggttcaccac gagcaacgga gctgtcaaac
    39541 acggtgccgt cgatcagttt accggtgtaa tgaacacgaa cgcggtcggt acgtgccgga
    39601 attgcgcctt caccctggtt gatcacgcgg aattgcaggc cagattcggt gctattcaca
    39661 ccttcttttt tggcgttttc ttccaggtat ttcacacctt cagcagccat cgcctggaaa
    39721 cgctgacgac gaacggcatc ggcgcgctcg tggatttcac gcagcgcgcg atgcaccaca
    39781 tcaaccggaa cagccggatg tttgccttcc agcgcatcgg caatacctgc aaccagtgct
    39841 tctggcagca gcccttccag gccagattca ctcagttgtt gccctacctg caaaccaatg
    39901 ccgtagcttg cttgcgcttc gatggtgtca aaagttgggg tggtcatcgt atttcctttc
    39961 ttagggtata aagtagcggg caggatatca gccacgcttc ttctggtaaa actttgtcgc
    40021 gggatgagtg acaaatccca gggaaacaga aacaataggg ataatccgca tgaatacaaa
    40081 aacagcggta ataccacgga catcatagcg ttagcttatt actgcgctat gattgaggaa
    40141 cacgacgcgg agctggagga aaaccatgcc tgggcgcttt gaactaaaac caactctggt
    40201 gaaaatctgg cacgcaccag ataactttcg cctcatggac ccgttgccgc cgatgcatcg
    40261 ccggggaatc attattgcgg caatagtgct gattatcggt attcttctgc ctggagatga
    40321 aacacgccag atgcctgttg tcacccgcga agcgcagtta gatatgcaat cacccccccc
    40381 aggtgaagca cagctacagg cacatttagt gacgccgcaa aacgatccgg atcaggtcgc
    40441 cccggtcgcg ccggaaccta ttcaggaagg tcaacctgaa gagccacctg ttgcagagac
    40501 acaatctttc caaccggaaa gtggtatcga tcaacagtgg cggacatatc gcgtagaacc
    40561 aggcaagacc cttgcccagc tgttccgcga ccataatttg ccgcctacag atgtgtacgc
    40621 tatggcccag gtcgagggcg cggataaacc gctcagtaac ctgcaaaatg gtcagattgt
    40681 taaaatccgt caaaatgcga atggggtggt taccggctta accattgata cgggaaataa
    40741 tcagcaagta ctgttcaccc gccaacctga tggtaatttc atccgcgcac gctgattacc
    40801 cacgggcagg gttcaccaaa agccagctca ggatggtctc tttgatcact ctgcgtctgg
    40861 cttccaaagc ctcaggtgaa accatgctaa tgtgataaac catccccagt gtgtactggt
    40921 ttattaaata atatgcaccc agtgcggcga tattaatatt gatttgtaag ggatcaatat
    40981 ctttcttaaa ggttttctgt cgctgccctt cttctaataa cgaagccatt gactgcaaat
    41041 gtgcatggtt aatttcagcg aggcgttttg attttttata atgcacaccc ttactttggt
    41101 tttcactatg gacaattttt aaaaaccacg gattattgat gtaataatcc caagtaaaat
    41161 caattatttt ctcgatcgca ataaccggag aaagccctga aatgtcaagc tctctctctt
    41221 tttcgcgaat atcgttccag gtatattcca gtacttcaat aaacaaattt tctttattac
    41281 caaagtggtg ataaaccgtc tgtttactac accctgctgc cttaacaata ttatctactc
    41341 tggccccttc ataaccatat tcggcaaatt cattaaccgc acatagcagc aatttttcct
    41401 tcaggttttg tgcgtgactg gtatcaagat acattccact tccccacttc ctcttcgtga
    41461 tggctatcat aaccggattt gatctcagcg acagcaaaaa ggataaaagt cgtaatttca
    41521 ttgagttatc aaattacctg tgatcactct cgcagatcaa ccaactagat ggttgatctt
    41581 tttacctgcc agtcgcaagc taatcatcac taaagaaaac aatattcaaa agagggttat
    41641 tgtatgaaat ccactcgtcc agttgccgtg attaccggag ccgctcgtgg tattggaaag
    41701 ggctgtgcgc tggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    41761 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcaga aggcgtggaa
    41821 gtcatttgct tccctgccga tgtcggcgat ctctccttgc atgaagagat gctcgacgcg
    41881 gcgcaaaacc agtgggggcg tctggactgc ctgctcaata acgccgggat ttcagtgaaa
    41941 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    42001 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    42061 ccggaagcgg aactgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    42121 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    42181 ctatttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg cccaggtctc
    42241 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    42301 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    42361 gcggaaggta aactgattta cacctgtggt caggcggtcg ccatcgacgg cggcttaagt
    42421 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    42481 atcaataccg caacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgct
    42541 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggcga agatctccag
    42601 cagatcgccc gtcagcttgc cgccagtaag atgaacgttt ccggtttgtg ccgtagcacc
    42661 tactacaccg caccgacgct ggcggagcgc aaactggcga ttgacgataa ccgccgggcg
    42721 ctggacgacg ccgctgtact gaatgccgcc tgttatatgc aggtggttgg cggtctgcca
    42781 acgggcacta aagatctata tgaagcgcgg gaacaagtga aacaagggat tcgccagttg
    42841 ctgccacatt cgaaagatgt cggcgtgcca attgcactgg agccgctgca cccgatgacc
    42901 gcagccgatc gttcctgtct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    42961 gatcccgatg gcgaatttgg cctcggcgtg gcggtagatg tctaccatgt ttggtgggac
    43021 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    43081 gactggttag tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc
    43141 atcaatattc cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa
    43201 ctggaaatat tctcgcctta ctggtggcag aaagatatta acagcacgct ggatattagc
    43261 gtcgatcgca tcgcgcatta ttgttaagga atgatcatga gtgaccaacc cgttctgttc
    43321 agcagagtgg cggcttcctg ccgcctgaca ttgaaccgtg aagataaatg tcatgctatt
    43381 aacgaagaga tgatcgaatc tctcgaccat tatttaaatg aaattgaaaa cgacaccaca
    43441 ttacgattag tcgaattaac ggcaaccggc gataaattct tttgtgcggg cggcgatatt
    43501 aaatcgtggt cggcctattc gccattagat atgggcagaa aatggattaa acgcggtaat
    43561 gacgttttta atcgcctgcg aaacttaccc caattaaccg ttgcgaatct caacggtcat
    43621 accatcggcg gtggtattga actggccttg tgctgcgata ttcgtattgc ccgccccggc
    43681 gcgaaattct ctaacccgga agtgatgctc ggcatggtgc ctggctggat gggcattgaa
    43741 cgtgtgctta atcaggttgg tccggtggtc ggtcgccaga tgctgatgct gggtaaacgc
    43801 ctgacggcgc aggaagccca ggcggcgaat ttaattgatg aagtggtaga aatagaacag
    43861 gtggaaagct ggatggcgaa ccagttagcg cagctggaaa aatgcggtcc ggtggcactg
    43921 gcgcatatta agcaactgat tctggcactg gaaaataaac atgccgatta cccgcaccag
    43981 ctactggccg gattaatgtc cgccactcag gattgccagc aggcgacgcg cgcctttgcc
    44041 gaaaaaagta gcgtgagctt ccataatcaa taaggaaatt gtatgcgtaa gataacaacc
    44101 gccgaggcac tggcggcgca aattcaggac ggcgcaacca ttgccattag cggtaacggc
    44161 ggcggcatgg tggaagccga ccatattctg gctgctattg aagcgcgttt cctgcaaacc
    44221 ggacaccctc gcgacctgac gctaatccac tcgctgggta ttggcgatcg cgactgcaaa
    44281 ggcactaacc gttttgcgca cgcagagatg ctcaaacgca ttattgccgg gcactttacc
    44341 tggtcgccca aaatgcaggc gctggtaaaa aataacacga ttgaagccta ctgctttcct
    44401 ggtggcgtga ttcaggcact gctacgggag atcggcgccg gacgtccggg gctttttacc
    44461 cacgttgggc tgggatcgtt tgttgatcca cgcaatggcg gcggtaagtc gaatgaatgc
    44521 actaccgacg acctggtaga actgattgaa atcgacggtg aaaccaaact tcgttatcgc
    44581 cctttcaaag tggattacgc gattttgcgt ggcacttatg ccgatcctcg aggcaacgtc
    44641 agccttgaag aagaagcgat tgatatggat agctattcca tggcgctggc agcacacaac
    44701 agcggcggca aagtgttcgt acaggtacgc gatgtgctgg aagctggcgc gattgaacca
    44761 cgccgggtca aattaccggg cattctggtt gatggcatcg ttgaacaccg cgaacaacca
    44821 caaacctatc ttggcggtta cgacctgacc atcagcggcc aacatcgccg tctgagttct
    44881 aacgacgcca ttgaactggt tagtcatccg gtacgtcgcc tgattgcccg tcgggcggca
    44941 cgcgaactgg tagctggcgc ttcaaccaac tttggctttg gtattcctgg gggtattcca
    45001 ggcgtagcgc tgcgcgaagg cgtgccttac caaagtttat ggctgagcgt cgagcaaggc
    45061 gtgcataacg gtatgatgct ggatgatgcg tttttcggct gcgcccgcaa cgccgatgcc
    45121 attattccat cactcgatca atttgagttc tacagtggcg gaggaatcga tatcaccttc
    45181 ctcggcatgg gagagatgga tcagtacggt aacgtcaacg tatcccatct caatggcaat
    45241 ctcattggtc ccggcggttt tctcgaaatt gcgcaaaacg cccgtaaagt ggtgttctgc
    45301 ggcacgttcg acgccaaagg tagcaagatt gatgtaacgc cagatggctt gcatatcacc
    45361 cagtcaggtc aaatccctaa actggttacc caggtggaaa aaatcacttt tagcgccgcc
    45421 tacgcacagc aaagtggtca ggaagtgttg tatatcactg aacgcgcagt attccagtta
    45481 acggcagaag gcgttgaatt aattgaaatc gcgccgggtg tggagattga gcgcgacatt
    45541 ctgccgtata tggctttccg tccaattatc aagcacccac gcctgatgga aagtagcctg
    45601 tttacgccga tggaggatgc atgagtcagc ttgatgacac catccttgat gcgctgacgc
    45661 acgttacttt cccaaagggt tttgcacagg cagagcccgc atgggttgtc acggtagacg
    45721 gtgttgatta cccactctgg caaacagatg ccctggtagt cggcagtggt gcagcaggat
    45781 tacgtgcggc agttgaactt aaacgccgcc agcaaaatgt gctgatcgct accgccgggt
    45841 tatatatggg gacgtcggca tgttctggtt ccgataaaca gacactattt accgccgcta
    45901 ccgcgggcaa cggcgacaac ttcaccaaac tggcagaggc actggcgagc ggtggggcga
    45961 tggatcacga caccgcttat gtcgaagcgg taggttctct gcacactcta ggcgggttgc
    46021 aatatcttgg tctggaatta ccggaagatc gctatggcgc gattcttcgt tatcaaaccg
    46081 accatgacaa agccgggcgt gcaacctcgt gtgggccgcg tacctcaagg ttaatggtga
    46141 aagtgctgtt ggaagaagta cagcgcctcg ccattccagt gttgaccagt gcaacagtga
    46201 ttaaactgct gcatcagcgt gacgaaaacg gcgaagaccg cgtggcaggg gcaatcctcg
    46261 cgaccggtca tcgcgcccat aacccatggg ggctggcaat tgtgactgcg cccaatgtgg
    46321 tactggcaac gggcggaccg ggcgagcttt atcgcgacag tgtttatcca cataaatgct
    46381 tcggctcgct ggggctggcg ctggaagaag gtctgacgct aaccaatctg accgaaagcc
    46441 agtttggtat aggcacgccg cgtagcacgt ttccgtggaa tttatccggc acctatgtac
    46501 aggtgatccc gtatatctat tccgtggatg ctgagggtaa cgagtataac ttcctcgcgg
    46561 attactatcg caccacccag gagctggctt caaacatttt ccgtaaaggc tatcagtggc
    46621 cgttccacgc cacgcgggtg atggattttg gctccagctt gttagatatg gcagtagcgc
    46681 aagagcagca atcagggcgt caggtattta tggatttcaa tcgcaatcct gaagcggtgc
    46741 cgggtgacct gccgttctca ttagatcgac tggacgacga cgttcgcgcg tatctggaaa
    46801 ataacgacgc tctggcacca tcgcccatcg aacggctgca acgaatgaat ccgctgtcta
    46861 tctcgctgta taagatgcac ggttacgatc tcaccacaca gcctttgcag tttgccatga
    46921 ataatcagca tatgaatggc ggcattgaag tggatatctg gggacaaacc tccctgcccg
    46981 gctgtttcgc cgtgggggaa gttgctggca cacacggcgt cactcgccct ggtggtgcag
    47041 cattgaatgc cgggcaggtt tttgctgttc gtctggcacg ttttattggt tgcacgcaaa
    47101 agcggaatat tgatggagat atagcacagc tggtagctcc tacactggct tctataagag
    47161 agatcattac tcaggcgcac gataacggga ccgggatgcc gttgtcggtt gtgagagaaa
    47221 aaattcaggc acgaatgtct gaccatgcgg gatttatttg ccatgccgat aaagtccgac
    47281 gcgccactcg cgatgccctg ctattgagcg aatttgtcca acggcatgga ttggctatca
    47341 aacatgtggg tgaagttgcc gagctgttta tgtggcggca tatggcactg acttctgccg
    47401 ccgtattaac gcaattaacg cattatattg acgcaggtgg tggtagtcgg ggagcacgta
    47461 ttattctcga cagggatgga aattctattc cgcaaacacg aaatggtttt tgtgatgcct
    47521 ggcgctttcg ttcagagcgt actgaggata aaaaagataa actacttatt cattattgca
    47581 acggtatctt tcacgtcaga gaaacacctg ttcgtgagtt tcctattatc cgaggtattt
    47641 ggtttgaaaa aaactggcct ggttttctta atgggacaat ttaccagcca caggatgaat
    47701 gatattgcaa aggttaatca ctatgcgaaa tataattaga tctattaatg ctctgcttgc
    47761 agcattgaac attaccattt tggcgatcat cgtggcatgt gtgacgtggc aggtggctgc
    47821 acgatttata tttactagcc ccagtatttt tacagatgaa ctcagccgtt tgctctttat
    47881 ttgtcttgga ctttttggtg gggcatacac cgcagggcaa aaccggcatc tggcgattga
    47941 cctgctacct atgatgctga aaggaaaagc ccgacgtcat ttatttttgt gtattcagat
    48001 tatcgttatt atcttcgcaa ccatcattat ggtatatggt ggtgggctat taacgatgga
    48061 tacattcgat tcaggtcaga cctcgcccgc tttaggctgg caaatgggat atatctatat
    48121 gagcatcccg attagtggtg tacttattat tatatacacc atagatatgg ttttaaccga
    48181 acttaaacaa ccactctaaa gataacaaac aacaaccaac catagtagga atggagaaat
    48241 tatggattac tggttaccga ttattgtgtt atttggtgcc ttctttttta tgttggcatt
    48301 aggtgtacct attgtttatg ccattggttt atcgacactg gcatcaattt ccacacaact
    48361 cgatttcaac tcagcattga gtgtggtttc ccaaaagtta gcctccgggt tagatagttt
    48421 tacgttgttg gcaatcccat tttttatctt gtctggcaac attatgaatc atggaggaat
    48481 agcgcgacga ttaatcaact ttgcccgtat tctcggcggt cgtctgccag gctcacttgc
    48541 ccactgtaat attctcgcca atatgctgtt cggggcaatt tctggttcag ccgtggcttc
    48601 ggcggctgcg atgggcggcg tcatgcatcc gcaacaggtt aaagagggct acgacccggc
    48661 attcagtact gcggttaacg ttgcctctgc cccgacgggt ttgcttattc caccaagtaa
    48721 tacgttaatc gtctattcac tggtcagtgg tggtacttct attgccgcac tgttcctggc
    48781 gggttatgtt ccggggattt tgctgggtct ggcactgatg gtaatagccg gtattatcgc
    48841 tgtacgtcgc ggttatccaa aaccagaacg tccgactttg cgccaggctg gcgttgcgat
    48901 atggatggct atccccagta ttttcctgat tgtgttaatt atgggtggcg tactaagcgg
    48961 tatttttact ccaaccgaag cctccgcaat tgcggtaatt tatacccttt tccttgcact
    49021 ggttctttat cgtgaaatct cagttaaaga tttacctaaa atatttctgg aatccgttat
    49081 taccactgca attgtcttgt tattaattgg ttcttcgatg ggaatgtcat gggcaatgtc
    49141 aaacgctgac gttcccttcc tgattctcga tttgcttaac actatttctg acaacccaat
    49201 aataatcctg cttattatca atattatcct gttgattatc ggcactttta tggatatgac
    49261 accggcggta ctgattttta cgccgatatt tttaccggtt gtcaccgaac tgggcatgga
    49321 cccgattcac tttggtattg tcatggtact gaacatgtgt atcggtattt gtactccgcc
    49381 tgtaggcagt gtgttatttg tcggttgtag tgtatctaaa ttaccgatta ataaaattat
    49441 caaacctatg ctgccattct atgccgtgat ggtattggtg ctggcaatgg tgacctatat
    49501 tccgcaaatc agtatggcat tgccaagagc actgggctac tgatgcgcat tcactggcgg
    49561 cacctgccgc catatttatt agaaaaggaa catctatgaa aataaaagta tccgctggca
    49621 ttatcggtgc tgttcttatg ttatccgcaa gccagtcctg ggcagtgaca ttaaaactga
    49681 gtcataatca ggataagtct catcctgttc ataaagcgat ggagttcttt gcgaaaaaga
    49741 gcaaagagta ctctaacggt gatattacta ttcgtattta tccaaatgga acattgggta
    49801 ctcaacgaga aacaatggag ctgattcgtt ctggcgctat tcccctggta aaaaccaatg
    49861 cggcagaaat ggaagcattt gaaaattcct ataaattatt tagcctgcct tatttgttcc
    49921 gcgatcgtga tcattattat caggtcatgc agggcgatat cgggagaaaa atcctcgact
    49981 caacgaaaag caaaggttat ttcgggctga ctttttatga tggaggcgcc cgcagtttct
    50041 atggcaataa accagtactg aaaccagacg atctgaaagg catgaaagtc cgtgtccagc
    50101 caagccctgg cgcagttgaa atgatcaaag tcatgggcgg taacccgacg ccactggatt
    50161 acggcgagtt gtatacagcc ttacagcagg gtgtggtcga tatggcagaa aacagcgtga
    50221 tggcgctgac taccatgcgt cacggtgaag tggcaaaatc cttcagcctt gacgaacaca
    50281 ctatggttcc cgatgtggtt ctgatgagca atgctgcgtt tgataaactt agcccggaaa
    50341 atcaggcagt tatattaaaa gcagctaaag aatcaatgag ctacatgaaa gacttgtgga
    50401 gcgaggaaga gaaacaagaa tttgcaaaac tggataaaat gggcgtgaaa gtctaccagg
    50461 tagataaagc tccgtttatc gagaaagtac agccaatgta cgcaaacttc gctaaggata
    50521 acccagccct tgccccaatg ctggctgata ttcaggcggc taagtaatta tcttcgtagg
    50581 gctgcttatt gtggccctta tcgatatctg caagtgctcg ctacaaaagc aaaacgccga
    50641 ccaatggtcg gcgttttttt acgtctcgtt gaataacgaa ttattcagct actacgttta
    50701 cgatcacttt cgcgaatact tcgctgtgaa cctggaagct cacttcgtgt tcgccagtgg
    50761 tacgcagaac gccgttcggc agacgaactt cgctcttagc cacttcaacg ccagctgcag
    50821 ttacagcgtc agcgatgtcg cgagtaccga tggaaccgaa cagtttacct tcgtcgccag
    50881 ctttagacgc gatggtaaca gtttccagtg cattgatttt ctcagcgcga gcattagcag
    50941 ctgccagaac ttcagccagt ttagcttcca gttcagcgcg acgtgcttcg aagaattcaa
    51001 tgtttttctt ggtagctgga acagctttac cctgcggtac caggaagtta cgagcatagc
    51061 ccgctttaac gtttacctga tcacccaggc tacccaggtt tgctacttta tcaagcagaa
    51121 taacttgcat taccttatcc tctcaaagtc gtattaatgg accgtgaccg attactgatg
    51181 gcgatcagtg tacggcagca gggacaggta gcgagcgcgt ttgatagcgc gagccagctg
    51241 acgctggtat tttgcacggg taccggtgat acggcttggg acaatcttac cgctttcggt
    51301 gatgtagttt ttcagcgtag cgatatcttt atagtcgatc tcttgaacgc cttccgcggt
    51361 gaaacggcag aacttgcgac gacggaaata acgtgccata tggctagtct ccagaatcta
    51421 tcaattcaat ctgctcggca tgcaaaacca ttttgctcag tccgttcttt gccttgtggc
    51481 atgaaatgaa cccctgaacg gttatgcgac tgccgaccgt tatactgtga gtaatggcct
    51541 ggttttcgtg tccgctaaca ataacgggca tttgacacca tgcctgccgg tgaaagccgg
    51601 cttcctcctg cacagaacga tgctcaagca cgaactggca gtgaggaatt cctgatggac
    51661 tgacctttcg aaggggagtc ctgcacacgg tgccggacaa caccagacgg ttggtcatca
    51721 gaaattactc ttcagaatcc ccagcttcag catcatcagc ggtttcgttt gcgaaatcat
    51781 cgcgacgctc acggcgctcg tctttcgctt taaccatcgg agatgcttcg gtaacagcgt
    51841 gcttggtacg cataaccatg ctgcggataa cggcatcgtt gaagcggaag gtagtttcca
    51901 gctcatcgat cacttcctgc ggagcttcaa cgttcatcag aacgtagtgt gctttgtgca
    51961 gtttgttgat cgggtaagcc agctgacggc ggccccagtc ttccagacgg tggatcttgc
    52021 cttctgcacc agtgatggca gcagtgtagc gctcgatcat gcccggaacc tgttcgctct
    52081 ggtcaggatg gaccataaaa acgatttcgt aatgacgcat cgaattgctc cttacggatt
    52141 attcagcctc ctgtctgggt cagccgaatc ccggggaggc aaggaacgtg ttaaaggtcg
    52201 gctgaaaaat gacgcgttat agtacttgta tgccccgcta aactcaaggc cagccgacaa
    52261 ataaatctca tctttcgctc aaaagaacta actccctgat ttgaagttca tcacattccg
    52321 tcacagtgtt tttttgaaca actgcatcag aaatggtcac catcaacttc tttatcattg
    52381 attaaaatta aatcagaaga tcatcacaga ggagagcaca caaagtcagc cgtcgaggag
    52441 gtatctatgt tcagtcgagt tttagccctt ctggctgtgc ttttgctaag tgcaaataca
    52501 tgggcagcca ttgaaatcaa taaccaccag gccagaaata tggacgatgt gcaaagctta
    52561 ggcgtgattt atatcaatca taatttcgcc actgaaagtg aagcacgtca ggcattaaat
    52621 gaagaaacag atgcgcaggg cgcaacgtac taccacgtaa ttctgatgcg ggaaccgggg
    52681 agtaacggca atatgcacgc cagcgcggat atttatcgct agcaccaggt ataaccaacg
    52741 aaacattgcc atagtttgct ttgccccctt cgcagggggc tttttttgaa aaacggctgc
    52801 ggaattagac cagttatctc ccgaagaagg aaatttccgc agcgcgtgtt ttacttctgc
    52861 ccgtaataag cgttaggacc atgcttacgc atgaagtgtt tattcatcag gaagctgtcg
    52921 atgtgattga gttgtggatt aatactgcgc gcaatccacg ccattttcgc cacttcttcc
    52981 atcactaccg cgttatgcac cgcgtcgtgg gcatctttcc cccaggcgaa cggtccgtgc
    53041 tgatacacca caattcccgg cgtatgcagc ggctcggcgt tgcccagcgt ttcgataatc
    53101 actttgccag tgttcagttc atactcaccc tgcacctctt cttcgcttaa tccacgcgta
    53161 cacgggatgt caccaaagaa gtagtcagcg tgcgtggtgc ctaacgccgg gatcgccagc
    53221 cccgcctgcg cccatgcggt agcatgagtg gagtgggtat ggacaatgcc gccaagcgac
    53281 ggataacgac ggtagagttc aagatgcgtc gcggtgtcgg aagatgggcg atactcccct
    53341 tccaccacct taccgctcat atcgaccacc accatatcgt ctgctttcat ggtttcgtag
    53401 gcgacgccgc tgggcttgat caccaccagc ccgcgttcgc ggtcgatagc gctgacgttg
    53461 ccccaggtaa aggtcaccag cccgtagcgc ggcagatcca tgttggcttc aaatacctgc
    53521 tgttttagct tttgcattat gccgcctcca ccatgcccgc tttcgccatg cgcgctttca
    53581 cccaatcacg cgctttcgcc acttctgccg ccgggtcttc cgccgtttcg ctccacatct
    53641 caatcaggta cggcccgcaa tagccactct gtttgagcgt ttcgaaacaa cgttcgaaat
    53701 ccactacacc ttcgccaaac ggcacgtttt tgaagacgcc aggtttggtg tctttcacat
    53761 gtaccgcgac gatatgcccg attccggcct gcaactccat ctgcacatcg ttgtcccacg
    53821 ccgacaggtt gccgatatcc gggtagagct ggaaccacgg gttgttgaga tagtgcgcgt
    53881 aacccagcgc cttgctgatg gagttcatca acggataatc catgatctcc atcgccagcg
    53941 tcacctgcgc gcggctcgcc atctcaacgc tctctttcag gccgtcacgg aaacgacgac
    54001 gcgtttcgtt attggcttcc tgatagtaaa cgtcatagcc cgccagctgg atcacgcgaa
    54061 tgccaacgtc ctgggcgaac tggatggctt tacgcataat ctccagcccc tgcgcccgca
    54121 ccgcgtcatc ttcgctgccc aacggaaaac gacgatgggc agaaaggcac atggacggca
    54181 cacgcacgcc ggtttcaaca atcgcattca ccagcgccag acgctgctcg cggctccagt
    54241 cgaggcgcga caggcgctcg tcagtttcat ctaccgacat ctcgacaaaa tcgaagccca
    54301 acgtttttgc cagtcgcagg cgttccagcc agcactcccc ggcggggagc gctttttcat
    54361 agatgccaag cgggatttgt ttggacaaca tatccgctcc ttagccccac agttcggcga
    54421 tggaacgttt gaactgacgc gcggcttcaa ccggagaagc ggcatcacgg atgctgcgac
    54481 cggcgataaa cacatgaatt ggaatgcctt tgaacagcgg cagatcttcc agcgccaggc
    54541 caccggtgac ggtgactttg aagcccatat cggaaagacg tttgatcgca gtgatatcgg
    54601 cttcacccca tgccacgcca gcggcctgcg cgtcacggct gcggtgataa accacctgct
    54661 gaatacctgc atcgcgccac tgttgcgcct gttcccaggt ccagtaaccg gtcagttcga
    54721 tctgcacgtc gccgttaaac tcttttgcca cgtccagcgc acctttggcg gtgttgatat
    54781 ccgcacaaca aatcacggtc acccagtcag cgttggcttc gaagcacata cgcgaaagga
    54841 ttttgcctgc atcggcaatt ttggcgtcag ccagtacgat tttgtgcggg tagagcgctt
    54901 tcagatcgcg aaccgcacgc acgccttcgc ccacgcacag aatggtaccc acttcgataa
    54961 tgtcgacttc ttcggcaatc aggcgagtgg tttcgtaggc gctatccatt gtctggttgt
    55021 ccagcgcgac ttgcaacatc ggtaatgaca tgttcaattc cttcttaagc tgccgcgttg
    55081 gtgcggtcaa tgagatccag tacttcctgc tcggtacggc aggcacgtaa acggtcgaaa
    55141 ttttcttcat cttcaaacag gttgacgatc tgcatgatgc ccacttcctg gtgcgtgttg
    55201 gcatcgaccg ccgccatggt gatgaggata tccaccgggt cgttatcttc gtggttgaac
    55261 tccagcggct ttttcagcgt caccagcgca aaaccggttt tcttaacgcc ctcttccggg
    55321 cgaccgtgcg gcattgccag gcctggtgcg agcacaaagt acggaccgtg ctgttcaacc
    55381 gcatccagaa tcgcctggta gtaacgcggc tcgacaacat ccgccgccac cagcaggtca
    55441 acgccgattt ttactgcgtc ctgccatgtc tcagcttcag cctgcaggcg gatggattta
    55501 ttctccgcca gcgaatcacg taatttcatg gcgcgtcctt acttcacatc ctgcgggaaa
    55561 tgcgctttga tcacttccag cagtttcggg ccaaagtcgg caggagagag catgttgcgc
    55621 acgccaacca cgtatttgtt gccggtcacg gtgatttcgc ccgcaatgtg cgtagaagcg
    55681 atgatgatat ccgcgccgct caactcgctt ttgtattcgc caaccgcgca gctatttacc
    55741 gtgtggtcaa tgtttgattg ggttaaaaac tggtccactt tcatcttcat gatcatggaa
    55801 ctgccttgtc cgttgccaca cacagccaga atacgtacgg tcataatcaa aactccttat
    55861 taagcagact gttctgccag ttgtttttct gcatcttctt ctgcgcgcag tgcgcggcca
    55921 gcgaagaaca tataagccag tgcaattaca atgatgacgg ccataaaggc gataccgatg
    55981 gagaagaagc cctgcatcat cggcggtgcc agaatcgacc agtccgccat gcccatccag
    56041 gcgctcatac cggtgagttt caccgcccag acgcaaccaa agatttcgat catccccatc
    56101 accagacaaa tcttcagcgc cgcacgccag ccgccgaagt ggttagcgaa cacgccgatg
    56161 gtggcgttgg agaagaacat cgggataaag ccgggaataa tcaggatcga ggaaccgcag
    56221 gcgaccagga tgccaaccgc aatcagctga ccgatggtgc cccacataaa gccccaaacc
    56281 acggcgttcg gcgcgaagct atagatagcc gcacagtcaa tcgccagaac cgcaccaggg
    56341 atcaggcgct gggagatacc gttaaatgct tcagagagtt ccgccacaaa catgcgcacg
    56401 ccctgagtga tgatgaagat cgccaccgcg aaggagaaac cagtttgcag gatgtacacc
    56461 gtccagttca ctttgcctgc catcgcctgc acggtgtcga taccgaagga gagcagaatg
    56521 gcaccaaaga agatggtcat cacaatcgcc gtggagacga tgttgtcgtg gaaaatattc
    56581 agccagcctg gcagtttgag gtcttcaacg ctttcctctt ttttgccgag gaacggcgca
    56641 actttatagg caatccatga tgcaaactgt tgctggtgac cgatggagaa accgcagccg
    56701 tccgtcactt cctgagtcgg cttgtacatc atgttggagg taatgcccca gtagagcgaa
    56761 accagaatcg ccgtgcagat aatggttgtc cacatggagt agccgaaaat aaacaacgta
    56821 acggcaatca gccccgcctg ctggaacatg atatgaccag tcagcatgat ggtacgaata
    56881 ccggtaatgc gacgcagcag cacgtaacag atgttcagcg ccagcgctaa caacaccgcg
    56941 taacctaccc agctataggc atcgcccatg cggtcgatgg ttgccatcat cgacgcgtaa
    57001 gtatcggaaa ttgcgccgtt aatgccgtag acttcggaca ttttcgccac caccggtttg
    57061 aaggtgctgg tgaggatgcc ggaccctgcc tgcaacaaca tgaaaccaat tatggtttta
    57121 atcgtgcctt taataataac gctgacactt ttgcgcagta ggatgtagcc cagacaggtc
    57181 acaataccca gcaacaacgg ggcattggtc atgacctggt taaaaaacac ggtaaagatg
    57241 ttgtagagga tctccataac gatctcctga agaagaggta accgggtatt ccgcacaccc
    57301 ggaatgttgt gcactcactc taattttcaa aagtaatcac aacaagatta tttatgatta
    57361 aatgtgacgc gacccgcaaa taattccaca cggatagagt gggattcaca caacgaaaca
    57421 attatttatt taaattcatt tagttaattt aactacacat tccattaaat ctttccacac
    57481 tcaaaatcaa caacaacttc cctggcaaaa agtcaaaaaa tggtcattgc cttcctgaaa
    57541 aaatagtgtc aggattaatc aaaaccaatc acatattgat tcgaattgaa ctatcatgaa
    57601 ggtaaatggc gatgagtaaa gtgaaaagta tcacccgtga atcctggatc ctgagcactt
    57661 tcccggagtg gggtagctgg ttgaatgaag aaattgaaca agaacaggtc gctcctggca
    57721 catttgcgat gtggtggctt ggctgcaccg ggatctggtt aaaatcggaa ggtggcacca
    57781 acgtttgcgt tgatttctgg tgcggcactg gcaaacaaag tcacggtaac ccgttaatga
    57841 aacagggtca ccaaatgcag cgcatggctg gcgtgaaaaa actgcagcca aacctgcgca
    57901 ccaccccgtt tgttcttgat ccgtttgcga ttcgccagat cgacgcggta ctggcgactc
    57961 acgatcacaa cgatcatatc gacgttaacg tcgctgctgc cgtgatgcag aactgtgctg
    58021 atgacgtacc gtttatcgga ccgaaaacct gtgtggattt gtggattggc tggggcgtac
    58081 cgaaagagcg ttgcatcgtg gtcaaaccgg gcgatgtagt aaaagtgaaa gacattgaaa
    58141 ttcatgcgct tgatgctttc gaccgtactg cactgatcac cctgcctgcc gatcaaaaag
    58201 cggctggcgt actgccagat ggcatggacg atcgcgcggt gaactacctg ttcaaaacgc
    58261 ctggcggctc cctgtatcac agcggcgact cccactactc taactattat gcgaagcacg
    58321 gtaacgaaca tcagatcgat gtggcgttag gatcgtatgg tgagaacccg cgcggtatta
    58381 ccgacaaaat gaccagcgcc gatatgctgc gtatgggtga agccctgaat gcgaaagtgg
    58441 ttatcccgtt ccaccacgac atctggtcaa acttccaggc cgatccgcaa gagatccgcg
    58501 tgctgtggga gatgaaaaaa gatcgtctga agtatggctt caagccgttt atctggcagg
    58561 ttggcggcaa atttacctgg ccgctggata aagacaactt cgagtaccac tatccgcgcg
    58621 gtttcgatga ttgcttcact attgaaccgg atctgccgtt caagtcattc ctgtaatctg
    58681 tcgtttatac cggatagcgt ggagaagttc gcgctatccg gcgtagtatt tcctggctat
    58741 ttcaaatatc atctaaaaaa atcaaatttt atcggaatag ctcatgactg aagcacaaag
    58801 acatcaaatc ctcctggaaa tgctcgcaca attgggcttt gtgaccgttg agaaagtcgt
    58861 tgagcgtctg ggaatttcgc ctgccactgc gcgacgcgat atcaataaac ttgacgaaag
    58921 cggcaaactg aaaaaggtgc gcaatggcgc agaagctatt acccagcagc gcccgcgctg
    58981 gacgccgatg aatctgcatc aggcgcagaa tcacgatgaa aaagtacgta tcgctaaagc
    59041 agcctcgcag ctggttaatc cgggcgaaag cgtagtcatc aactgcggct ccaccgcgtt
    59101 tctgcttggg cgggaaatgt gtggcaagcc agtgcaaatc atcactaatt atctaccgct
    59161 ggcaaattac ctgatcgatc aagaacatga cagcgtgatc attatgggtg gacagtacaa
    59221 caaaagccag tccatcactt taagcccgca gggcagcgaa aacagtctct atgccgggca
    59281 ctggatgttt accagcggaa aagggctgac cgcagaaggg ttgtataaaa ccgatatgct
    59341 gacagcaatg gcagagcaga agatgctgag cgtggtaggg aaactggtgg tactggttga
    59401 tagcagtaag attggcgaac gcgcgggaat gctttttagc cgtgccgatc aaatcgatat
    59461 gctgatcacc ggcaaaaatg ctaacccgga aatcctgcaa caactggaag cgcaaggggt
    59521 cagcattctg cgtgtttaaa gatgctggcg gaaaaatgtc acggcagcat ctaacgcctc
    59581 aggcgtaatg cggtgacgca cgcctggctg ccatgaacag gttagcagtt tatcccgtcc
    59641 cgtctcgctt aaggcctgct gcaaacgtag tgattcgtcg gcaggcacaa cgtcatcgtc
    59701 gaggccatgc cacagcagta gaggtctgtc cccaagttgt tccaggtggt ttgtcgcttc
    59761 ccactctgcc agcggcgcga taatgttatt gaattcattc tgctgtgctg ccgtttcagg
    59821 tatcagcggt ggaaacagtg aacgggcgag cgatgtaaaa tagcccgatc ccatcatgct
    59881 ggcggtacat ttcaccgtgg ggtggcgagc ggtaattccc agtgccgtca tcgcgcccat
    59941 cgacgcgcca ccgactgcca gacggtcatc aagcagccag ttttcttcgg ctattgccgc
    60001 acgtaaagta gtgaattcct gcatactttg tagcaagatt tgccagaatt gatttaaccg
    60061 ccgcgcttcg tcgctactaa aacggctacc gtgatcgggc gcatccggca tgatcacccg
    60121 caaaccagcc tgcgccagcg caacggcaaa atagctatac accaggctgg atgaagtaaa
    60181 gccgtgataa aaaattacgc acggtaacgg ggtatctttt tgcccgacag gataagcatg
    60241 aagaacggga atatctgcca gctcgcgtga ttctatttca atcatctgtc tctccttttt
    60301 gtctggtatg acatgaagaa atccttagca caagtttctg ctggcggtat tagttcataa
    60361 tgttgagatg tgggttacgc tttcgtcacg ttttcattcg aaaatcgcgt accaggtaac
    60421 aattcgggaa cattccccca aagttaaatt aataaggcac tacactatgg ttagcaggaa
    60481 acgtaatagc gttatttacc ggtttgccag tttattattg gtgttgatgt taagtgcctg
    60541 tagcgcactg caaggtacgc cacagccagc accgccagta acgaaccatc cgcaagagat
    60601 tcgccgcgac cagacgcaag ggttacagcg aataggtagc gtaagcacga tggttcgggg
    60661 ttctccggat gacgcattag cagaaatcag agcaaaagct gtcgctgcaa aagctgatta
    60721 ttacgttgtc gtcatggtgg acgaaaccat cgtgacagga cagtggtatt cacaagccat
    60781 tttgtaccgt aaataaatca gacaacttac atagtcagct ttacattgct ttgcgtccct
    60841 atgcgtttac ctgtgcacaa taaattaccg gctggaagcc tgggcgttta ttaccgcgat
    60901 ggaatgccct gcaacatgtg gggaaacgaa aaatggagct gacgatgaaa caattacttg
    60961 cctcaccctc gctgcaatta gtgacttatc ctgcgagcgc cacggcacag tctgcagaat
    61021 tcgccagtgc tgactgcgtg acgggcctga atgaaatagg tcagatctca gttagcaata
    61081 tttcagggga tccgcaggac gtggaacgta tcgtggcgtt aaaggccgat gaacaaggtg
    61141 cgtcatggta ccgcattatt accatgtacg aagaccagca gcctgataac tggcgcgtac
    61201 aggcgatact gtatgcatga gtgtcatcta cagcaaatga acaatgccgg atgcggcgcg
    61261 agcgccttgt ccggcctaca attttcacat atttcattga gttaatcgaa accagcgtcg
    61321 catcagtcgt atacgcttac acacacactc cccccgtcgc ccgcagcaat aaatcattct
    61381 ggatctgctc tgacaaccgt acgccgccgc gcgtatctaa catcacctga caccacgcct
    61441 gtgccattgg cggagaggca tatttcaaca tttgcgcacc gcagcccagc aggaatagct
    61501 gatgagtaat ctctcgcccc aattcttcag ctggcttgcg cagctgctgc tgtaaacgac
    61561 gaaccgcgcg atcaaaatag cgatcctgcc ctttcacttc cacaaacgct tccgacaata
    61621 agtcgtatac gcccgcttgc ttattgagaa cgcgcagcac atccaggcac ataatattgc
    61681 cggaaccttc ccaaatactg tttaccggca tctcccggta aagccgcggc agctcgctct
    61741 cctcgcaata accaatgccg cccagcacct ccatcgcttc ggccacaaac ggcatacctc
    61801 ttttgcagat cacaaatttt gccgcaggcg taaataaacg cgcccacagg gcttctttgg
    61861 catcggcacg ccggtcccac gctcgcgcaa gacgaaacag caacgccgtt tgcccttcaa
    61921 gctgaagtgc catgcgactt aagacatgac gcataagggg ctgttggatc aatggattac
    61981 caaaaacatg gcgttgatgt gcatgataaa tcgccagcga aaatgcacgg cgcatcatgg
    62041 catggctacc cagggcgcaa tcaaaacgcg tcatcccacc cattttcagg atcagacgaa
    62101 ttccttcccc ttcctgcccc aacaaccagc caatggcatc ctgaaactcc acttcgcagc
    62161 tggcgttaga gcgattaccc agcttatctt tcagccgctc gaggcgaatc gcgttgcgtt
    62221 gcccgtcagg caaaaagcgc ggcacaaaaa agcaggacaa accgcccgta gtctgcgcca
    62281 gcaccagatg cgcatcgctt tgcggcaccg agaaaaacca tttatgcccc accagccgat
    62341 aagagccatc ttccaggcgc tctgcgcggg tggtgttgct catgacatcg gaaccgccct
    62401 gcttttccgt cattcccatg ccaatcaaca aaccgcgttt ttgcccacct ggcaataagt
    62461 gagaatcgta gcgatcgctc agcagcggcg tggtccagtc ctgaaacggc gcgggtaaca
    62521 tctgtaacag caatggcgtg gcggcaaagg tcatggttat cggacataac gaccctgcct
    62581 caacctgtgc gtgcaacata aaacgcgccg cgcgcgccac aaatgcgccg gagcgagcgt
    62641 cttcttccca ggcaagattg tgcacccgat tggtacatag cgcctgcatc agcaggtgcc
    62701 aggcggggtg aaaacgcaca tcgtccagac ggcgtccttg cgcatcgtag cgcaataatt
    62761 caggcggatt cacattcgcc agccgcccca gttcaaggga ttcagccgtt cctaactgct
    62821 gaccaatact ggctagaaaa tcgctatccc agccagcacc ttcacgcgtt accgcttcgc
    62881 agagcgcgcc atcagacagg tataagttac tgttatttaa tggtataggt tgattaaaaa
    62941 cggtgtgagt ttgccagtgc actgtgtctc cctccatcaa tggcagtcac cattagtatg
    63001 gtcactgcca tggattcatg acagaaagag attgctaaaa cattcttatc cagcaatgta
    63061 atgcggaacg aaacgtgagg tgtctttggt gatcagtgtg ttatcttcac ggatccccat
    63121 tccgcacgct tcatcatcga caatccagct accgatgagt gtgtagctat cgccaaatcg
    63181 cggcagaggt tgaaacgcct ggtagatcat cggttcatcg gcgtaatcac catcagcgtg
    63241 gtcaacgacg ttattctgac cgtcgaaaat ggtgacgttt ccgccttcgc gcgagtagat
    63301 cggtttacgc acatagcttt cgccagcggc aatctgcgat ttttcaccct caaaccagga
    63361 tgcaagaagg ttaggatgac cagggaagaa gcgccaaagc agcggcatta gccctttatt
    63421 actcaagata cttttccata acggctccac ccattgctcg cgacgcttgc acagcagcgg
    63481 accgttatcg tcacgcatca tccactccag cggatacagc ttaaatgcac gctggatgac
    63541 attatcatca agatcggtca gtacaccgcc gacgcccaaa ccgagatctt caatgtagat
    63601 aaaccgcgac tcctgccctg cctgctgggc gcagtcctgc aagtacagca cggtagtcct
    63661 gtcttcatcg gtgtcctgac agcagcagaa ataaaacggc tcccggctat aaagctcgct
    63721 aaagcgtgaa atcaggcgtt cctgaatggc attgtactga tcggcatcac gcggaataat
    63781 gccgctgcgc cgggcatcct ccagccacaa ccactggaaa taagccgact catacaacga
    63841 ggtcggcgta tcggcgttgt actccagcag cttcaccggc gcattgccac accaggcaaa
    63901 atccatccgg ccatacagcg aaggatcgcg tgcgcgccag ctttcggcga tcacatccca
    63961 gtacaacggc gggatcgcca gttgcgtcag gatctcttcg tctttaaccg cgcgatccac
    64021 cacctcaagg cacatctgat gcagttccgc agtcggtttt tcgatctgct cttcaatctg
    64081 gcgcagagta aaacggtaag cccgactctc gtcccaatag atttcattgt cgatgatatg
    64141 aaagtcgaaa ccgttgttag cggcgatctg gtccagatcc cgtcgcacag gaacgttgtg
    64201 tctcagcatg attagcctcc ccaatgccca cgggcgctgg aagaacgacc atagccgccg
    64261 cgcgaaacgg ttgacgcttt tttggtggta aagccgcctg aagagtaaga ctcttttttg
    64321 ccagaaccgg agcgccagga gtaatcaccg gaagtgttgc gccagacggg gcgggaagca
    64381 aatgaggatc cgccgctgtt atagacgaac ggctcatcgc gatctttacg aataactcgg
    64441 cttaacaaaa atccagaaac gaccgggatc caactctgtt caacattgtc gtagtagcaa
    64501 ttttcgtact tagactgaca attctgctga gtcatattct tcggaacatc ggcataaaat
    64561 gccgctttgg cgttgttcca gccacgcgcg caaatgtcgg cattattacc gtcatcaata
    64621 caatcctgca ctgtcgaata aaacgttccg tcgccgtcgt tatcgacatc gccactttca
    64681 ctccatcctt tgactattaa aaaaacggca ccgcccatta gcaccaacgt taagtatttt
    64741 tgtgcgtagc gattgcggcg cggctcaaaa ggattatgcg gtcttccaat gcgactgatc
    64801 gctccgtgac caattttact gttatttctt gatttgcgtt ttctggccat gatcgtccat
    64861 taccatgtca tacaagctgc gttaaaaata ccaccagcca gtgcggcggt tcccataaac
    64921 attcctgctg cggtattgtg attaataatt ttttcgctta atgcgggcat ataaagtctg
    64981 acaccagcaa aaacgagtaa ctgaatcacc agtgcaatcc cgccccaggc gaaatagtct
    65041 ggaatactca ccgcattgat tgccgcactg gataagggga tcacgtaacc caacaatgta
    65101 ccgctgaatg ccagtgacgc cgcggtatta ttgtttttga ttaattgcca ttcgttgtgc
    65161 ggtgtaattt tggagtagat aaacagaaaa ataatcacca tagccacgcc aataaaaaaa
    65221 taggcactaa aggcaagaag tgaatccagt atatgcattg taaacgtcct tgtaattaac
    65281 caataatatg aagtgatgtc agcgggatat cgacacccaa cgcgcgcgaa aataaccact
    65341 ctggctcgcc gagatcgtta aaagattcct caccatttaa cagcaaatat tcgtaagtat
    65401 cttcggttac ttggcgttgg tagcccatgg taaaattatg gacctcccat ttggcatggt
    65461 tttgattttc tactttttcc agcatgtaga cgggttcgat atttcccggt tcttcgctgt
    65521 taaaaaatcg ctgccagcgt ttttcctgcc agtttaagat cattgccccc atcactttgg
    65581 cgttgatcgc ctcgcgccag tgactctctt tactgatacc gtagctctct tcatagacaa
    65641 agagcttgat atcatcaatg tcatcaatat cttcgccgcc ggtggtattg atttgcagaa
    65701 attcatcgcc tgaagtgtag tagcggaaaa tctgactacc gccgcccaga tcgatgtggc
    65761 tgacagcggc taccgtaaat tcctcacccg gcagcgcaat cagcaattca tcttccagca
    65821 aacgaaacgc taacgtgtcg agcgtaaagc cactattgag atgaagtccc agcggaccgc
    65881 gagcgatagc aggcttatta tccttgccaa acagacgctg gaaaaaacca gacatatttt
    65941 atccttaaaa tagttgccag ccttttagcg aggctggcaa aaaaattact cgccctgttg
    66001 gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc caattccggc
    66061 ttccgccagc ttttcatcaa gatcgcgacc gtctgcgact ttttccaact gtgcagcagc
    66121 atccaggcga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg attctgccgc
    66181 tgtcgaaacg ctggaagatg cgccaacggt agaggttgtt acagcctgtt gtgcacgctg
    66241 catggcttca gtggctttaa cgacttccat ttgctgctca aactgagcga tacgctgcgc
    66301 tgtcgctgta actgcttttt ccacgccatc acgggagact tccagattcg acaacacttg
    66361 ctcttcagcg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt cgttaatcaa
    66421 cgacggatta acgttcttgc ttaacgcttc cagcgcacgg gcttccagac tggctttgcg
    66481 ctcacgtaaa tctttcagct tatcgtgact taatttcacc cgcgccagca gatcaacgcg
    66541 agattttccg gctttatcga gttcagcttt agcgtcacga atatgctgtt ccagcatgcg
    66601 cacgccctgg gtttcttcaa tggattcttc cgcctgggag ataaacgatt tccccagcgt
    66661 aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg aatattcttc
    66721 tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa gcaggatatc
    66781 ttcaagagaa gatttaagcg ataacgcccc gaaaacaatg taatactctt cctgttgcac
    66841 gctggagatt cctaccgatg acaacggcat catcttctga tttcttaata agaaggtatt
    66901 aaattcatct ggattgctga tactacttac cggacaaata aaggtttcga taatcat
//
LOCUS       DAPGWD010000006        65990 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPGWD010000006 DAPGWD010000000
VERSION     DAPGWD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.006"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..845
                     /locus_tag="RHX88_000342"
     CDS             <1..845
                     /locus_tag="RHX88_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDV2229076.1"
                     /translation="WSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANS
                     TRKYWAVGGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSY
                     FIKIDFDLLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQN
                     CVRVTNIETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            958..1752
                     /gene="panB"
                     /locus_tag="RHX88_000343"
     CDS             958..1752
                     /gene="panB"
                     /locus_tag="RHX88_000343"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDV2229077.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1764..2615
                     /gene="panC"
                     /locus_tag="RHX88_000344"
     CDS             1764..2615
                     /gene="panC"
                     /locus_tag="RHX88_000344"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDV2229078.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2689..3591)
                     /gene="rpnC"
                     /locus_tag="RHX88_000345"
     CDS             complement(2689..3591)
                     /gene="rpnC"
                     /locus_tag="RHX88_000345"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HDV2229079.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            3865..4245
                     /gene="panD"
                     /locus_tag="RHX88_000346"
     CDS             3865..4245
                     /gene="panD"
                     /locus_tag="RHX88_000346"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDV2229080.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4249..5478)
                     /locus_tag="RHX88_000347"
     CDS             complement(4249..5478)
                     /locus_tag="RHX88_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDV2229081.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5542..5982)
                     /locus_tag="RHX88_000348"
     CDS             complement(5542..5982)
                     /locus_tag="RHX88_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV2229082.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(6087..6857)
                     /locus_tag="RHX88_000349"
     CDS             complement(6087..6857)
                     /locus_tag="RHX88_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2229083.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6854..7780)
                     /locus_tag="RHX88_000350"
     CDS             complement(6854..7780)
                     /locus_tag="RHX88_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2229084.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7889..8551
                     /gene="can"
                     /locus_tag="RHX88_000351"
     CDS             7889..8551
                     /gene="can"
                     /locus_tag="RHX88_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDV2229085.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8592..9128)
                     /gene="hpt"
                     /locus_tag="RHX88_000352"
     CDS             complement(8592..9128)
                     /gene="hpt"
                     /locus_tag="RHX88_000352"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDV2229086.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            9334..11724
                     /gene="gcd"
                     /locus_tag="RHX88_000353"
     CDS             9334..11724
                     /gene="gcd"
                     /locus_tag="RHX88_000353"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDV2229087.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQL
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11771..13321)
                     /gene="cueO"
                     /locus_tag="RHX88_000354"
     CDS             complement(11771..13321)
                     /gene="cueO"
                     /locus_tag="RHX88_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDV2229088.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13487..13834
                     /locus_tag="RHX88_000355"
     CDS             13487..13834
                     /locus_tag="RHX88_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDV2229089.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13940..14806
                     /gene="speE"
                     /locus_tag="RHX88_000356"
     CDS             13940..14806
                     /gene="speE"
                     /locus_tag="RHX88_000356"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDV2229090.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14822..15616
                     /gene="speD"
                     /locus_tag="RHX88_000357"
     CDS             14822..15616
                     /gene="speD"
                     /locus_tag="RHX88_000357"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDV2229091.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15654..16016)
                     /locus_tag="RHX88_000358"
     CDS             complement(15654..16016)
                     /locus_tag="RHX88_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDV2229092.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16192..18789)
                     /gene="acnB"
                     /locus_tag="RHX88_000359"
     CDS             complement(16192..18789)
                     /gene="acnB"
                     /locus_tag="RHX88_000359"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDV2229093.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            19144..20901
                     /locus_tag="RHX88_000360"
     CDS             19144..20901
                     /locus_tag="RHX88_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDV2229094.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20972..22396)
                     /gene="lpdA"
                     /locus_tag="RHX88_000361"
     CDS             complement(20972..22396)
                     /gene="lpdA"
                     /locus_tag="RHX88_000361"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDV2229095.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22721..24613)
                     /gene="aceF"
                     /locus_tag="RHX88_000362"
     CDS             complement(22721..24613)
                     /gene="aceF"
                     /locus_tag="RHX88_000362"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDV2229096.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAPDAAPAQAEEKKETAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24628..27291)
                     /gene="aceE"
                     /locus_tag="RHX88_000363"
     CDS             complement(24628..27291)
                     /gene="aceE"
                     /locus_tag="RHX88_000363"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDV2229097.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27452..28216)
                     /gene="pdhR"
                     /locus_tag="RHX88_000364"
     CDS             complement(27452..28216)
                     /gene="pdhR"
                     /locus_tag="RHX88_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDV2229098.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28757..30130
                     /gene="aroP"
                     /locus_tag="RHX88_000365"
     CDS             28757..30130
                     /gene="aroP"
                     /locus_tag="RHX88_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDV2229099.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTHFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30173..31027)
                     /gene="ampE"
                     /locus_tag="RHX88_000366"
     CDS             complement(30173..31027)
                     /gene="ampE"
                     /locus_tag="RHX88_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDV2229100.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(31024..31575)
                     /gene="ampD"
                     /locus_tag="RHX88_000367"
     CDS             complement(31024..31575)
                     /gene="ampD"
                     /locus_tag="RHX88_000367"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDV2229101.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            31663..32556
                     /gene="nadC"
                     /locus_tag="RHX88_000368"
     CDS             31663..32556
                     /gene="nadC"
                     /locus_tag="RHX88_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDV2229102.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32759..33199
                     /gene="ppdD"
                     /locus_tag="RHX88_000369"
     CDS             32759..33199
                     /gene="ppdD"
                     /locus_tag="RHX88_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDV2229103.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            33209..34594
                     /gene="gspE"
                     /locus_tag="RHX88_000370"
     CDS             33209..34594
                     /gene="gspE"
                     /locus_tag="RHX88_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDV2229104.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            34584..35786
                     /gene="hofC"
                     /locus_tag="RHX88_000371"
     CDS             34584..35786
                     /gene="hofC"
                     /locus_tag="RHX88_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDV2229105.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35821..36864)
                     /gene="guaC"
                     /locus_tag="RHX88_000372"
     CDS             complement(35821..36864)
                     /gene="guaC"
                     /locus_tag="RHX88_000372"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDV2229106.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(37020..37064)
                     /locus_tag="RHX88_000373"
     CDS             complement(37020..37064)
                     /locus_tag="RHX88_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229107.1"
                     /translation="MIIKTLKMSARKRN"
     gene            37089..37709
                     /gene="coaE"
                     /locus_tag="RHX88_000374"
     CDS             37089..37709
                     /gene="coaE"
                     /locus_tag="RHX88_000374"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDV2229108.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37709..38452
                     /gene="zapD"
                     /locus_tag="RHX88_000375"
     CDS             37709..38452
                     /gene="zapD"
                     /locus_tag="RHX88_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDV2229109.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38462..38659
                     /gene="yacG"
                     /locus_tag="RHX88_000376"
     CDS             38462..38659
                     /gene="yacG"
                     /locus_tag="RHX88_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDV2229110.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38759..39148)
                     /gene="mutT"
                     /locus_tag="RHX88_000377"
     CDS             complement(38759..39148)
                     /gene="mutT"
                     /locus_tag="RHX88_000377"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDV2229111.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39208..41913)
                     /gene="secA"
                     /locus_tag="RHX88_000378"
     CDS             complement(39208..41913)
                     /gene="secA"
                     /locus_tag="RHX88_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDV2229112.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41975..42487)
                     /gene="secM"
                     /locus_tag="RHX88_000379"
     CDS             complement(41975..42487)
                     /gene="secM"
                     /locus_tag="RHX88_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDV2229113.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42718..43635)
                     /gene="lpxC"
                     /locus_tag="RHX88_000380"
     CDS             complement(42718..43635)
                     /gene="lpxC"
                     /locus_tag="RHX88_000380"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDV2229114.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43736..44887)
                     /gene="ftsZ"
                     /locus_tag="RHX88_000381"
     CDS             complement(43736..44887)
                     /gene="ftsZ"
                     /locus_tag="RHX88_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDV2229115.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44948..46210)
                     /gene="ftsA"
                     /locus_tag="RHX88_000382"
     CDS             complement(44948..46210)
                     /gene="ftsA"
                     /locus_tag="RHX88_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDV2229116.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46207..47037)
                     /gene="ftsQ"
                     /locus_tag="RHX88_000383"
     CDS             complement(46207..47037)
                     /gene="ftsQ"
                     /locus_tag="RHX88_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDV2229117.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(47039..47959)
                     /gene="ddlB"
                     /locus_tag="RHX88_000384"
     CDS             complement(47039..47959)
                     /gene="ddlB"
                     /locus_tag="RHX88_000384"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV2229118.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47952..49427)
                     /gene="murC"
                     /locus_tag="RHX88_000385"
     CDS             complement(47952..49427)
                     /gene="murC"
                     /locus_tag="RHX88_000385"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDV2229119.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49481..50548)
                     /gene="murG"
                     /locus_tag="RHX88_000386"
     CDS             complement(49481..50548)
                     /gene="murG"
                     /locus_tag="RHX88_000386"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDV2229120.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50545..51789)
                     /gene="ftsW"
                     /locus_tag="RHX88_000387"
     CDS             complement(50545..51789)
                     /gene="ftsW"
                     /locus_tag="RHX88_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDV2229121.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51789..53105)
                     /gene="murD"
                     /locus_tag="RHX88_000388"
     CDS             complement(51789..53105)
                     /gene="murD"
                     /locus_tag="RHX88_000388"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDV2229122.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGLTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(53108..54190)
                     /gene="mraY"
                     /locus_tag="RHX88_000389"
     CDS             complement(53108..54190)
                     /gene="mraY"
                     /locus_tag="RHX88_000389"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDV2229123.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54184..55542)
                     /gene="murF"
                     /locus_tag="RHX88_000390"
     CDS             complement(54184..55542)
                     /gene="murF"
                     /locus_tag="RHX88_000390"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDV2229124.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55539..57026)
                     /gene="murE"
                     /locus_tag="RHX88_000391"
     CDS             complement(55539..57026)
                     /gene="murE"
                     /locus_tag="RHX88_000391"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDV2229125.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(57013..58779)
                     /gene="ftsI"
                     /locus_tag="RHX88_000392"
     CDS             complement(57013..58779)
                     /gene="ftsI"
                     /locus_tag="RHX88_000392"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDV2229126.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58795..59160)
                     /gene="ftsL"
                     /locus_tag="RHX88_000393"
     CDS             complement(58795..59160)
                     /gene="ftsL"
                     /locus_tag="RHX88_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDV2229127.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(59157..60098)
                     /gene="rsmH"
                     /locus_tag="RHX88_000394"
     CDS             complement(59157..60098)
                     /gene="rsmH"
                     /locus_tag="RHX88_000394"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDV2229128.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(60100..60558)
                     /gene="mraZ"
                     /locus_tag="RHX88_000395"
     CDS             complement(60100..60558)
                     /gene="mraZ"
                     /locus_tag="RHX88_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDV2229129.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(61160..62164)
                     /gene="cra"
                     /locus_tag="RHX88_000396"
     CDS             complement(61160..62164)
                     /gene="cra"
                     /locus_tag="RHX88_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDV2229130.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(62246..62332)
                     /locus_tag="RHX88_000397"
     CDS             complement(62246..62332)
                     /locus_tag="RHX88_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDV2229131.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(62344..62835)
                     /gene="ilvN"
                     /locus_tag="RHX88_000398"
     CDS             complement(62344..62835)
                     /gene="ilvN"
                     /locus_tag="RHX88_000398"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV2229132.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(62838..64562)
                     /gene="ilvI"
                     /locus_tag="RHX88_000399"
     CDS             complement(62838..64562)
                     /gene="ilvI"
                     /locus_tag="RHX88_000399"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDV2229133.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            64681..64725
                     /locus_tag="RHX88_000400"
     CDS             64681..64725
                     /locus_tag="RHX88_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229134.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(64880..65824)
                     /gene="leuO"
                     /locus_tag="RHX88_000401"
     CDS             complement(64880..65824)
                     /gene="leuO"
                     /locus_tag="RHX88_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDV2229135.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        15903 a        18177 c        16149 g        15761 t
ORIGIN      
        1 tgtggtcagc ttacggtacc gtaactaacg ttagttcacc tgggatatac attggtgact
       61 ctgcagaaca atatttttcg tggtataatc caagcgaaga cgtgttatat tggagttgca
      121 ataatgcgaa tagcacccgt aaatactggg ctgtaggtgg tatttatcag acccttacaa
      181 ttgaattcta tacagataca aactttgatc caacagtaac tcaacagatc aaattatcca
      241 gctcatcaaa ttatctgtat tcatttaaag cctatggcgc aggccaaggt ataaatgagc
      301 atagttattt tatcaaaatc gatttcgatc tgctcaatgt caagttgact aatcccactt
      361 gttttaccgc tatgcttagt ggaacttctg tgactggttc tacggtaaaa atgggtgaat
      421 atagtgcaga acaaatcaga aacggtgcca caccggttcc ttttgatatt tcacttcaaa
      481 actgcgttcg tgtgactaat attgagacaa aattagtttc aacaaaggtt ggtactgaaa
      541 acgggcaact ccttggtaat actctcacag gtaatgacgc agcaaaagga gtcggtgtac
      601 tcatagaagg tttagcaact agtaaaaatc ctctaatgac attgaaacct aatgattcaa
      661 attctgttta taaagattac gacccgagag gcaaagacga tacaacagga ggagtttacc
      721 cggatcaaga tacaggtata acataccctc tccatttcca ggccacgcta caacaggatg
      781 gaactatacc aatagaagct ggtgagttta aagccaccag tactttccag gtaacctacc
      841 cttaataagt ccaccgcacc gccatcatct ggcggtgcgg taattgataa atcattgccc
      901 ccaccaatgt gactgataaa atgccaggca acacgacatc atttatcagg acacgttatg
      961 aaaccgacca ccattgcttc acttcagaaa tgtaagcagg ataaaaagcg tttcgcgacc
     1021 atcaccgctt acgactacag cttcgcgaaa ctatttgctg atgaaggact taacgtcatg
     1081 ctggtaggcg attcgctggg catgacggtt cagggccacg actccaccct tcccgttacc
     1141 gtcgaggata tcgcctatca taccgccgcc gtacgtcgcg gcgcgccaaa ctgcctgctg
     1201 ttggctgacc tgccgtttat ggcgtatgcc acgccggaac aagcctttga aaacgccgca
     1261 acggttatgc gtgccggtgc caatatggtc aaaattgagg gcggtgagtg gctggtcgaa
     1321 accgtaaaaa tgctgaccga acgtgccgtt cctgtatgtg gtcacttagg tttaacccca
     1381 cagtcagtga atattttcgg tggctacaaa gttcaggggc gcggcgatga agcgggcgat
     1441 caactgctca gcgatgcatt agccttagaa gccgctgggg cacagctgct ggtgctggaa
     1501 tgcgtgccgg ttgaactggc aaaacgtatt accgaagcac tggcgatccc ggttattggc
     1561 attggcgcag gcaacgtcac tgacgggcag atcctcgtga tgcacgacgc cttcggcatt
     1621 accggcggtc acattcctaa attcgctaaa aatttcctcg ccgaaacggg cgacatccgc
     1681 gcggctgtgc ggcagtatat ggctgaagtg gagtccggcg tttatccggg cgaagaacac
     1741 agtttccatt aaggagtcac gttgtgttaa ttatcgaaac cctgccgctg ctgcgtcagc
     1801 aaattcgccg cctgcgtatg gaaggcaagc gcgtggcgct ggtgcctact atgggtaatc
     1861 tgcacgatgg ccatatgaag ctggtcgacg aagccaaagc ccgcgccgat gtggtcgtcg
     1921 tcagtatttt cgttaacccg atgcagttcg accgcccgga agatctggca cgttatccac
     1981 ggaccttgca ggaggactgc gagaagctaa acaaacgtaa agtggattta gttttcgccc
     2041 cttcggtaaa agagatctac ccgaacggta ctgaaaccca tacttacgtt gacgttcccg
     2101 ggctttcgac catgctggaa ggcgcgagcc gtccggggca ttttcgcggc gtttcgacta
     2161 tcgtcagcaa gctgtttaac ctggtccagc cggacatcgc ctgcttcggt gaaaaggact
     2221 ttcagcaact ggcgctgatc cgcaaaatgg ttgccgatat gggcttcgat attgagattg
     2281 tcggtgtgcc aattatgcgc gccaaagatg gccttgcgct aagttcccgt aacggttatc
     2341 tgacggcgga acaacgcaaa attgcgcctg gtctgtacaa agttttaagt tcgattgctg
     2401 acaaattgca ggctggggaa cgggatctcg atgaaattat taccattgcg gggcaagaac
     2461 tgaatgaaaa aggcttccgc gccgatgata ttcagattcg cgatgccgac acattgctgg
     2521 aagtttctga aaccagcaaa cgggcagtaa ttctggtagc cgcctggctt ggcgatgctc
     2581 gcctgatcga caacaaaatg gtcgagctgg cgtaatactt aactggcgct acggctgatg
     2641 gcgccagtta ttaatttacc ccacgtcatc tgtcataaca caggtaactt aaataaggtt
     2701 aattacctta tcaatctcag caagagataa atttgtcatc tctgcaacgt cttcccgaga
     2761 cattccttta cttagaagac gcagggcgaa ttcttgcctt tcctcctgtc ttccctgctg
     2821 aatccccttc tcaatccctt tctcttcaaa ccactgcgcc agcgtcatca tagactcccc
     2881 tcccgtttcc ctgtctctca gcacaccata aaacaaatcc gcttgttcag tatgaccgcg
     2941 ttgcagcata tagttttgca tggcaactaa ctgacttccg ctagtgtacc cttcgtcaat
     3001 cagcgtgacc agttgctcca gcaataacat taagtcgcgc tggcgaatat gtttttgcag
     3061 tagttcgaga atcgcaatcc gccgatgttg catgatttcg tcatccggtg tgatggtgat
     3121 atccaccagc gggaaaggac tgttatagac gcgtcgtgcc agctccggcg agtaaaatat
     3181 atcaaaccag cacattgaga gtggataagg tgtggcctcg ccctgataaa acagtatcgg
     3241 caccaccagc ggcagcttat cgtgatccgc ctccagatgc cggtgcatgg cagcaataga
     3301 ataacgcatc atgcgaaagg ccattttctt atccggcttg ctttggtgtt caatcacaac
     3361 atgcagataa ccgggattgc cctgcatttg cacggaatag agcacgtccg tgctgtgtcc
     3421 tttcaggctc tcttcaatga aactccccga ctctaaatga agcgtgttga ggtcacaaag
     3481 ttctcgtaat tccactggca aatgtatctc cagaaagtcg cgagccgtct ccgcatgcat
     3541 taaaaattgt ttaaataccg cgtcatgcgg tgtggtactc ggtgcatcca tcttccctgt
     3601 cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg cgcgccgtcg tcagccgcct
     3661 ctttagtgtt taccgagcag cgttcagaga aatttcaatt cgttacaggc gatacatggc
     3721 acgcttcggc gcggtacgca aactgctgac gcaaatgcca tcagatgagt agacactcat
     3781 caaaaatcgg gcaatactgc gtgagaattt cctcaaagca ggcaccgcct gcttcgttaa
     3841 cgacagggta gaaaggtaga agttatgatt cgcacgatgc tgcagggcaa actccaccgc
     3901 gtgaaagtga ctcatgcgga cctgcactat gaaggttctt gcgccattga ccaggatttt
     3961 cttgacgcag ccggtattct cgaaaacgaa gccatcgata tctggaatgt caccaacggc
     4021 aagcgtttct ccacttatgc catcgcggca gaacgcggtt cgagaattat ttctgttaac
     4081 ggtgcggcgg cccactgcgc cagcgtcggc gatattgtca tcatcgccag cttcgttacc
     4141 atgccagatg aagaagctcg cacctggcga cccaatgtcg cctattttga aggcgacaat
     4201 gaaatgaaac gtaccgcgaa agcgattccg gtacaggttg cttgattgtt atccctgcgg
     4261 ctggttactc accagccgcg acatcgtctc cagcgaatcc gttcttaaga tataaagtcg
     4321 ttttagtaac aacggattat cccccggttt tactttgcct ttcatggttg tcaccgccag
     4381 gtgaaatccg gcatcgtttg ctgccttcac ggctttgtca ttaaatccgc caaacggata
     4441 cgaaagatac cagacatgcg gattaaattg cgccagagcg cggcgtgaac gtgcaaaatc
     4501 aaacagaata ttgtgctcac tacggctcag taatatgggt cggcgatagc catccacccg
     4561 atgcaaaaaa tgggtatgtg actgaaaatc aaaaacatcg cgaatttcgt taagctcaga
     4621 aacgctcata aattgcagcg attttgggtt ccacttctgc gggtgacgtt tgatgcgcga
     4681 ggtaacaaca aacgccgtcg ccttcatgcc atattgtttc aatacaggat acgcatagcg
     4741 gctcaccgac ttgaggccat catcaaaggt aatcaccacc gctcgcgcag ggagattgat
     4801 cttattcttc acgtaacctt ccagctgcgc catgctcagt gtcgcgtatc ccctgtcgcg
     4861 cagccaggcc atctggttat tgaaagcgcg taccgaggtg gtcgtcgaag tatggcgaaa
     4921 acgggtgttt tcttcatcgc gcagaatatg gtgataggtt agcaccggca ggccattatc
     4981 gggttgggca tccagcgcgc tgatataggc gagtcgatcg ccaatacgga tctgatacca
     5041 ggtttgattt aacctgtctt tcagtttatg caaaatcggg tagcgcaaat tgtccgccag
     5101 taccccaaat ggcgcactgc ccgcactcgg cgcgttatag accggcgtat ctttccaggt
     5161 aattaagttc tgattactca gcggcttgtt gaggtcaccc aaaccgtctt caactttttg
     5221 tcgcccctga actggctcga gatgcccttt atcgataaac cctttaccaa agccaaaatt
     5281 aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg atttgcccgg cccgaatatt
     5341 ccccacggtc accatcttgt cgccaatttg cgcccagatc gcagcatttt cgatggtttg
     5401 catataacgg gcaggtaacg cggcactgac acttgcggta aacagcatca gcaggagaat
     5461 aacagcttgt ttgtacatga aaaaataacc gagggtgaaa caacgctttt attgtagcaa
     5521 aagcgctata agaatcgtgg gttaatactc atacaaatta tgtttgagga cgaaaggcgg
     5581 atttttttgt tgttggtgcc aaagactact cacctccggc ccacccagtt cgacaatacg
     5641 ctcgcgaaac tctgaactgg tcatggtttc acggcaagcc atcatctgtt caattaacgg
     5701 taacgtgaca ccagaaatca cttcgcaacg ggagtgtttg tggcttaata atgaagccac
     5761 gcggtacggt ggcgctccgg ctatatcggt taagaagatg acaccctcac cagagtccgc
     5821 ttcatgcaga gcatcgcaca tcatgcggct gagcatatta gagcttaacc cacgccagaa
     5881 attcacggcc cggcactgaa gaagcgcccc atgttttttc tccagcgcat ccagtatctc
     5941 ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac atcgccttcc tccttagccg
     6001 aaaatcagtc taccctgacc attgttgata aagggtgata gccgtcaaaa ctcctctccc
     6061 ccaaatccag aggagaggaa atagccttag ctacgcaaac cacgtccacg ttggatcagc
     6121 gaccaacaga tcaaataaaa cgccacaata aagaccacca gtacgccaaa ggtagtgacc
     6181 agcggaacat cattgatacc gaggaagccg taacggaaac cactgatcat ataaacgatt
     6241 gggttcaggt gcgacagccc ttgccagaac ggcggcaaca aagtcagtga gtaaaagacc
     6301 ccacccaaat acgtgagtgg cgttaacaca aaggttggca ccaggctgat gtcatcgaac
     6361 gttttggcaa acacaccgtt cagcaaaccc gcaagggaga acaacaccgc cgtgagcacc
     6421 agggttaagg caacgaatac ccacgaatgc acctgaaacg gcacaaaaaa cagtgaaatt
     6481 gccgtcacca gaatgccaac aaacagacca cgcgccacgc caccgccgac atatccggca
     6541 ataatgacgt gagtcggaac cggcgctacc agcagctctt caatattacg ctggaacttg
     6601 gcaccaaaaa atgatgacgc aacgttggcg taggcattgg tgatcaccga catcatgatc
     6661 agccccggta cgatgaactg catatagctg aagccatgca tatcgccaat acgcgaacca
     6721 atcaggttac cgaagataat aaagtaaagg gtcatggtga tgactggcgg caccagcgtc
     6781 tgcacccaga tacgcataaa gcgatggatc tctttcgccc agatgctttt tagcgccacc
     6841 cagtaaagat gcatcatgcg cgatctcctt gtttttcatt aaccagtgaa acaaacagct
     6901 cttccagacg gttagctttg ttacgcatac ttaatacctg aatgccctgc tcacttaact
     6961 gcgtaaatac gctgttgatc ccctgctcac gcagcacttc aacttccagc gtcgcggtat
     7021 cgaccagtcg atactgatag ccatcgagct tcggtaacgg acttttcggt gcgagatcga
     7081 gaataaaggt ttccgatttc agcttcgcca gcagcgcctt catcgaggta ttttccacca
     7141 gctcaccgtg ttgaataatg ccgatattgc ggcacagcat ttctgcttct tccaggtagt
     7201 gtgtggtgag aatgatggtg gtgcctttgt cgtttaaatc cttcaaaaag ccccacattg
     7261 agcggcgaag ttcaatatcc acgcctgcgg tcggttcgtc gagaatcagt agtttaggtt
     7321 catgcattaa cgcacgggca atcattaaac ggcgcttcat cccgccagat aacatacgcg
     7381 cacgttcgtt gcgttttccc catagatcga gttgtttaag atatttttcg ctgcggatgt
     7441 acgcctcttt gcgctccaca ccgtagtacc ctgcctgatt taccacaatt tgctgcacgg
     7501 tttcaaacgg gttgaagtta aattcctgcg gcaccagtcc caactgacgt ttagcgttca
     7561 cgacatcttt ctcgagatcg taaccaaata cgctgacccg cccggaggtt ttatttacca
     7621 gagagctgat aataccgata gtggtcgatt tcccggcccc gttcggcccg agaagcgcat
     7681 aaaaatcacc cgcttcgacc tgcaaatcta ttccacgaag cgcctgaacg ccgcctggat
     7741 aagttttttt aagctgttga agttccagtg caatggtcat aaatttttac ttaccttacg
     7801 ttcttacact ttatctgtgg tttaaatcgt cccggagttg ccctatatta gccaaacgta
     7861 attatttggt tacaggtcgt taacctccat gaaagacata gatacactca tcagcaacaa
     7921 tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg ttttttgaga aactggcaca
     7981 agcgcaaaaa ccgcgctttc tatggattgg atgttccgac agtcgcgttc ctgcagaacg
     8041 tttaaccggt cttgagccgg gcgaactctt tgttcaccgt aatgttgcta acctggtcat
     8101 tcacaccgac ctgaactgcc tttccgtggt tcagtatgca gtggatgtac tcgaagttga
     8161 acacattatt atctgtggcc actacggttg cggcggcgta caagccgcag ttgaaaaccc
     8221 ggaactgggg cttatcaaca actggctgct gcatatccgc gatatctggt tcaaacatag
     8281 ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat accttgtgtg aactgaacgt
     8341 catggaacag gtttataacc tgggccactc caccattatg caatcagcgt ggaaacgcgg
     8401 gcagaaagtt accattcacg gctgggccta cggcattcac gacggcttgc tgcgtgatct
     8461 ggatgtcaca gcgaccaacc gcgaaaccct tgagcaacgt taccgtcacg ggatttccaa
     8521 cctcaagctg aaacacgcca accacaaata aaaatgccat gccggatgca acacatccgg
     8581 caacttcaca cttactcgtc cagcagaatc actttgccga tatacggcag atgacggtaa
     8641 cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat ccgggatcga gaaaccgata
     8701 aattcaaccg ggacgttcac ttcacgacgg gacggtttat ccagcagcgt acaaatcgcc
     8761 agcgacttcg gttcgcgcag gcttaagatc tcacgcactt tcgacagtgt attccccgag
     8821 tcgatgatat cttcaacaat cagcacgtcc ttgccacgga tatcttcatc cagatctttg
     8881 aggattttca catcacgggt ggtggacatg ccgctaccgt agctggaggc ggtcataaag
     8941 tcgacttcat gagatacctg aacttcacgg cacaggtccg ccataaacat aaatgagcca
     9001 cgcagcagac caaccagcac catatcgctg ccgctgtctt tgtaacgctc agtaatctga
     9061 cgacccagtt cggcgatacg cgctttaatc tccgcttcgg ggatcattac ttctacagta
     9121 tgtttcatat ctctaaccat atgatttaaa aacaaatcac ttcgcacggg caggttttgg
     9181 caccgcaaca aagcgcaaga cacgcagtat accagtaaaa ctattcattc gcggaatctg
     9241 ttaataaagc tcgaattgtg atgacgatca cacatgttaa aacctataat taattgctac
     9301 taaaatatta atgaattgaa atggtgtctc tttatggcaa ttaacaatac aggctcgcga
     9361 cgattactcg tcacgctaac agcccttttt gcagcgcttt gcgggctgta tctactcatt
     9421 ggcggaggct ggctggtcgc gattggcggc tcctggtact accctatcgc tggtcttgtg
     9481 atgctcggcg tcgcctggat gctgtggcgc agtaaacgtg ccgcgctttg gctgtacgca
     9541 gccctgctgc tcggcaccat gatttggggc gtctgggaag ttggtttcga cttctgggcg
     9601 ctgactccgc gcagcgacat tctggtcttc ttcggcatct ggctgatcct gccgtttgtc
     9661 tggcgtcgcc tggtcattcc tgccagcggc gcagttgccg cactggtggt ggcactgctg
     9721 attagcggtg gtatcctgac ctgggccgga tttaacgatc cgcaggaaat caacggcacc
     9781 ttaagcgcca atgccacacc tgcggaagct atctcacccg ttgccgatca ggactggcct
     9841 gcctatggtc gcaatcagga aggtcaacgc ttttcacctc tgaaacaaat taacgccgat
     9901 aacgtccata atctgaaaga agcctgggtg ttccgtactg gcgatgtgaa gcagccgaac
     9961 gatccgggcg aaatcaccaa tgaagtgacg ccgattaaag tgggcgacac cctttacctg
    10021 tgtaccgctc accagcgcct gtttgcgctc gatgccgcca gcggcaaaga gaaatggcat
    10081 tacgatcctg agctgaaaac taacgagtct ttccagcacg taacctgccg tggcgtctct
    10141 tatcatgaag ccaaagcaga aacggcttcg ccggaagtga tggcggattg cccgcgtcgt
    10201 atcattcttc cggtcaatga tggtcgactg attgcgatta acgctgaaaa cggcaaactg
    10261 tgcgaaacct tcgccaataa aggcgtgctc aatctgcaaa gcaatatgcc agacaccaaa
    10321 ccgggtctgt atgaaccgac ttcgccaccg attatcaccg ataaaaccat cgtgatggcc
    10381 ggttcagtta ccgataactt ctcaacccgc gaaacgtctg gcgtgatccg tggttttgat
    10441 gtcaacaccg gggagctgct gtgggctttt gatcccggcg cgaaagatcc gaacgcaatc
    10501 ccgtctgacg aacacacctt tacctttaac tcgccaaact cctgggcacc agcggcctat
    10561 gacgcgaagc tggatctggt ctatctgccg atgggcgtga ccacgccgga tatctggggc
    10621 ggtaaccgca caccggaaca ggaacgttat gccagctcga ttctggcgct gaatgccact
    10681 accgggaaac tggcgtggag ctaccagacc gttcaccacg acctgtggga catggatctt
    10741 ccggcacagc cgacgctggc ggacatcacc gttaatggtc agaaagtgcc agttatttac
    10801 gctccggcga aaaccggcaa catttttgtg ctcgatcgtc gcaatggcga actggtggtt
    10861 ccggccccgg aaaaaccggt gccgcaaggt gctgcgaaag gcgattacgt tactccgacc
    10921 cagccgttct ctgaactgag cttccgtccg aagaaagatt tgagcggtgc ggatatgtgg
    10981 ggagccacca tgtttgacca actggtgtgc cgcgtgatgt tccaccagtt gcgctatgaa
    11041 ggcattttca cgccgccatc tgaacagggc acgctggtct tcccgggtaa cctgggaatg
    11101 tttgaatggg gcgggatttc cgttgatcca aatcgtgaag tggcaattgc caacccaatg
    11161 gcactgccgt ttgtttcgaa actgatccca cgcggtccgg gtaacccgat ggagcagccg
    11221 aaagatgcca aaggcacggg tacggaatcc ggcattcagc cacagtacgg tgtaccgtat
    11281 ggtgtcacgc tcaacccgtt cctctcaccg tttggtctgc cgtgtaaaca gccagcatgg
    11341 ggttatatct cggcgctgga cctgaaaacc aatgaagtgg tgtggaagaa acgtattggt
    11401 acgccgcagg acagtatgcc gttcccgatg cctgtaccgg tgccgttcaa tatgggtatg
    11461 ccgatgctgg gcgggccaat ctccacggcg ggtaacgtgc tgtttatcgc cgctacggca
    11521 gataactacc tgcgcgctta caacatgagc aacggtgaaa aactgtggca gggtcgttta
    11581 ccagcgggtg gtcaggctac gccaatgacc tatgaagtga atggtaagca gtatgtggtg
    11641 atctccgcag gcggtcacgg ttcatttggt acgaagatgg gcgactatat tgtggcttat
    11701 gcgctgccgg atgatgtgaa gtaaccataa tgcccggaga aatccgggca tatttccgaa
    11761 tacggtcttt ttataccgta aaccctaaca tcatccccgt atcttcatgc tccagcagat
    11821 ggcagtgcgc catataagca tgttctttcg gtgcgtcgtg attaaacttc accagcactt
    11881 cactgacatt gccttctacc ttaacggtat ctttccagcc cgcgcgatga gccgctggcg
    11941 gtttgccatt ttctgacaag atacggaact gggtgccgtg aatatggaac ggatgcagca
    12001 tcatgtcgcc cacgccagag ataacccaac gttcgtactg ccctttcgcc gccgcaaaca
    12061 tcggcttatt catatcaaac gcctgaccgt tgattttatt ggcatggtgg aaatcgaact
    12121 tcccgccgtg gttcatatga ttcatattgc cgtgccccat atggcccatc atctggctgt
    12181 gatccattcc agccatcgcc tgatcgccat atttctccat tagcatctgc atacccatca
    12241 tatcgagcat cgggtccata gaaagttgca gcttgcgtac cgtcagccct tccagcgaag
    12301 gtaacgcagg caggctactt aatgtgtctg gcaaagcacc ggaggcacta atagcaatcg
    12361 gctgaatccg cattaccgga tgcggcttgt caaacggcgc aatcgccatc cccatctggc
    12421 tgaccggtag cgtcaccagg tcaaagggtt tgttgtcgtt aacctccacc agcacttcaa
    12481 aacgctcgcc catcagcacc ggcagctcgc tcaccttcac cggttcaggt agcagaccac
    12541 cgtcgctggc aatcacatac agcgggcgat tgtcgctggt ggcgaaattg agcgagcggg
    12601 cattacagcc attgagcaaa cgcaggcgca gccaaccacg cggggcagcg tgttgtgggt
    12661 agattgcacc gttggtcagc agcgtatcgc caaaccagcc cacggcggcg gtcatcacat
    12721 ccagttgata atcaatctgc ccatcagcgt taaatttttt atcctgaaca atcaccggaa
    12781 catcatcgat accccactgt tttggcagca ttaatttcag gatctcgtca tcttcaatta
    12841 ccaccagccc agccaacccc atcgccacct gtcgcccggt cttgccatgt tgatgtggat
    12901 ggaaccagca agtagcggca ggttgatcaa cgttcaacgt caccgagcgc ttgccacctg
    12961 gcagaataat tccctgcggg ccgccgtcga cttcacccgg tacttccagc ccgtgccagt
    13021 gcaacgtcgt ctcttccgtc agttggttat agatatcaac cgttaccgct ttgccgcgct
    13081 gtaatttcac cgccggcccc agcagattgc cgttatagcc ccaggtagtt gcagttttct
    13141 cgccaaaggt agactgacct gcgccaatag ttaactgaat gcgattacgg gcatcggtcg
    13201 tgagcaaatc agggatcggt aacgttgggc gttccgccgc aaagactgcg cggctccaca
    13261 gcggcaaggc tgaagccaca cccagcgcga cggaatattt taagaaatca cgacgttgca
    13321 tagttatttc cttattctta agcaggcgaa acaaaatcaa acgttgagca tagtccttcc
    13381 ccttacggga aggtcaagcc gcagacacaa tttaatcgac aataatggtc gtcgcctcgc
    13441 tcgcagtgtg ctaacgttta tcttctttaa gcccctggta gaagcaatga agacgttttt
    13501 cagaacagtg ttattcggca gcctgatggc cgtttgcgca aacagttacg cactcagcga
    13561 gtctgaagcc gaagatatgg ccgatttaac ggcagttttt gtctttctga agaacgattg
    13621 tggttaccag aacttaccta acgggcaaat tcgtcgcgca ctggtctttt tcgcccagca
    13681 aaaccagtgg gatctcagta attacgacac cttcgacatg aaagccctcg gtgaagacag
    13741 ctaccgcgat ctcagcggca ttggcattcc cgtcgctaaa aaatgcaaag ccctggcccg
    13801 cgattcctta agcctgcttg cctacgtcaa ataatccctg ataccttttt gtagaaatat
    13861 tgaccgtgca tccgcggtca atgttagcta ttatgttgcg cccttttttt acgggtgtta
    13921 acaaaggagg tatcaaccca tggccgaaaa aaaacagtgg catgaaacgc tacacgacca
    13981 gtttgggcag tactttgcgg tagataacgt tctgtatcat gaaaagaccg atcaccagga
    14041 tctgatcatt tttgagaacg ctgcatttgg tcgcgtaatg gcgctggatg gcgtagtaca
    14101 aaccaccgag cgcgacgagt ttatctatca tgagatgatg acccatgttc cgctactggc
    14161 ccatggtcac gcgaaacatg tgctgattat cggcggcggc gacggtgcca tgctgcgtga
    14221 agtaacccga cataaaaacg ttgagtcaat cacgatggtg gaaatcgatg cgggtgtcgt
    14281 atcgttctgc cgtcagtatc tacccaacca taacgccggt agctacgacg atccgcgctt
    14341 taagctggtg atcgacgatg gcgtcaattt cgttaatcaa accagccaga cctttgatgt
    14401 cattatctcc gactgcaccg atcctatcgg tcccggcgaa agccttttca cttcggcatt
    14461 ttatgaaggc tgcaaacgtt gcctgaatcc tggcggtatc ttcgtcgcac aaaacggcgt
    14521 ctgcttttta cagcaggaag aagccatcga cagccatcgc aaactcagcc attacttcag
    14581 cgacgttggc ttttatcagg cggcgatccc gacctattac ggcggtatca tgacttttgc
    14641 atgggcgaca gataacgacg ccttacgcca tctctcaacc gaaattattc aggcgcgttt
    14701 tctcgcctct ggcctgaaat gccgttatta caatccggca atccatacgg cagcttttgc
    14761 cttacctcag tatctgcaag acgcactggc ttcacagccg tcctaaggag aagataagaa
    14821 attgaaaaaa ctgaaactgc atggctttaa taatctgacc aaaagtctga gtttttgtat
    14881 ttacgatatc tgctacgcca aaactgccga agagcgcgac ggttatattg cttatatcga
    14941 tgaactctat aatgccaacc gtctgaccga aatcctgtca gaaacctgtt ccattatcgg
    15001 ggccaatatt cttaacatcg cccgccagga ttacgaacca cagggtgcca gcgtcactat
    15061 tctggtgagt gaagaaccgg ttgacccgaa actcatcgac aaaacagaac accccggccc
    15121 actgccagaa acggtcgttg cccatctcga taaaagtcat atttgcgtac atacctaccc
    15181 ggaaagtcat cctgaaggcg gtttatgtac cttccgcgcc gatattgaag tctctacctg
    15241 cggcgtgatt tctccgctga aggcgctgaa ttacctgatc caccagcttg agtccgatat
    15301 cgtaaccatt gattatcgcg tgcgcggttt tacccgcgac attaacggta tgaagcactt
    15361 tatcgaccat gagattaatt cgattcagaa ctttatgtct gacgatatga aggcgctgta
    15421 tgacatggtg gatgtgaacg tctatcagga aaatatcttc cataccaaga tgttgcttaa
    15481 agagttcgac cttaagcact acatgttcca caccaaaccg gaagatttaa ccgacagcga
    15541 gcgccaggaa attaccgctg cgctgtggaa agaaatgcgc gagatttatt acgggcgcaa
    15601 tatgccagct gtttaacggc tctggcggag ctcccaggct ccgccagatt tatttacttc
    15661 tgctgcacga aattgcggta agccgccacg acctgcagaa aatcctcaac gccgcatagc
    15721 gacaggcttt cttcgtcgta gtagttcatc ccctcttcca tttcatcgcc agcgaattcc
    15781 agttgattgg cgcgaaccat cacctcttca ccgtccatcc acagggtgta ttcatgccct
    15841 gcccgttgcc aggaccgttc gctacctttc agtgcgtgcg ctgcttgttc cacttcatca
    15901 agcaaggcca ggttttcttt cacctcttca ttaaaccagt gcccgaccac ttcatgcccc
    15961 atggacatac gcacctttac cactccggta atatcgcgca gaaattcgta atccatagtg
    16021 tgttcctctg ctcccggcaa tgcgccgaaa tcgcattgcg ccattgatgt aattatcgca
    16081 gccttaccgg agaaaaacag cggaacggcg atggtttaga aataaaaaaa tgcccgggaa
    16141 tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt attacaccgc
    16201 agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt tgaagttcag
    16261 ataacgataa gtatcaacgg cagttttatc tacttgcgcc acgtaggtct ggtactcttc
    16321 cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttccgcag aagccaggaa
    16381 gacattcgcg ccagtaccca gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt
    16441 cgcaccgtct gctacacgcg cctggttacc catacacaga gaacagcccg ggatctcgat
    16501 acgcgcacca ctcttaccga agacgctgta gtagccttct tcagtcaact gcgcggcgtc
    16561 catacgggtt ggcggtgcca cccacaggcg ggtcggcagt tggcctttgt gcgcatccag
    16621 cagtttaccc gcagcacgga agtgaccgat gttggtcatg caggaaccga taaatacttc
    16681 gtcgatcttc tcaccctgta ccgcagacag cggacgcgcg tcatccgggt cgttcggtgc
    16741 acagaggatt ggctctttaa tatccgccag atcgatgtcg atcactgccg cgtattcagc
    16801 atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc cctgaatacg
    16861 acgttccagg gtgcgacgat cgccgtaacc ttccgcgatc atccacttca gcaggacgat
    16921 gttagagttc aggtattcga tgatcggttc tttgttcagc ttgatggtac aaccagcggc
    16981 agaacgctcg gcggacgcat cggtcagttc aaatgcctgc tcaactttca gatccggcag
    17041 accttcaatt tccaggatgc ggccagagaa gatgtttttc ttgcctttct tctcaacggt
    17101 cagcagacct tgtttgatcg catacagcgg aatggcatgt accagatcgc gcagggtgat
    17161 gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat ccagcggcat
    17221 tacgcctgtt gcggctgcaa atgccaccag accagaaccc gccgggaaag agataccgat
    17281 cgggaaacgg gtatgggaat caccaccggt accgacggta tccggcagca gcatacggtt
    17341 cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac ggttcataat
    17401 gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg
    17461 gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt ctttcagttc
    17521 atcacgggtc atcgggccgg tggtgtcctg ggaacctaca gaagtcattt tcggttcaca
    17581 gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt tttgcgccag
    17641 cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat cactgtgcgg
    17701 cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca gcggaatacg
    17761 gccaccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg tcgccagcag
    17821 ttcgccggtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa tcacgtcgcc
    17881 catgttcagg ttagagacgt cgacttcgat tggcagtgca cccgcgtctt ccatcgtgtt
    17941 aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt tgttcggcac
    18001 atgtggaata tcatcgccca taaaccacag aacggagtta gtggcggatt tacgcgaaga
    18061 acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc
    18121 gatttgcttg atcggaccaa caacaccagg ctggtctggc tcaatacctt cacgggcgtt
    18181 tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat ccggtgccgg
    18241 agaaaggtca tcggtgttag tttcgccagt gactttgaag acggtaacgg tcagtttttc
    18301 agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca taacctgctt
    18361 cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat cgaacatcag
    18421 cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat ccagcgcgtc
    18481 gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga tggctttttc
    18541 cggagtcagc agaggggatt tggcttcgcc tttcgcgata gcagccagga agcctgcttt
    18601 gacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat ctaacaggaa
    18661 ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agtgcggcca tttggtttgc
    18721 atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct tacggtattc
    18781 ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga ttctcttcac
    18841 gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg cagaatagca
    18901 ggattttgac gatgtgttaa tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg
    18961 ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg catggtgata
    19021 atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc tctttatact
    19081 cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct tgagatccga
    19141 attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg cagtgccggg
    19201 ctttgtgccg cctctaccgg gctatatata aaaagccgca cagtggaagc gcctgtggaa
    19261 ccgcaatcga cacaacagac tgcgcctgac atcaccgcag ttacgcttcc tgcaacggtt
    19321 tccgcaccgc ccgtaacgcc cgccgtcgtc aaatccgcat tcagcactgc acaaatcgat
    19381 caatgggttg cgcctgtcgc gctgtacccc gattctctgc tttcacaagt gttaatggca
    19441 tcaacctatc cggcaaacgt tgctcaagca gtgcaatggt cgcacgataa tccacttaaa
    19501 caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag cgttaaatca
    19561 ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac
    19621 ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca gcgattgcgg
    19681 caactggcgc aacaaaccgg ttcgctgaag tcatcaaccg aacaaaaagt tattaccaca
    19741 acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc atccaatacc
    19801 gttttaactg ccagccccgt cattacagag cctgcaacaa ccgtcatttc cattgagccc
    19861 gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta cgggaactgg
    19921 gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc gtttattgac
    19981 agctttgtgc gcggattcgg ctatagcatg ggtgttgcta ccacgtacgc actattcagc
    20041 agcatcgact gggatgacga cgatcatgac catcatcatc atgacgatga tgattatcat
    20101 caccacgatg gcggtcatcg tgacggtaat ggctggcaac acaacggcga caacatcaat
    20161 atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa gaatatggca
    20221 tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga tatggcaaag
    20281 cggtttcatc aaaccgatgt caacggcgga atgagcgcca cgcagctacc tgctccaaca
    20341 cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca cgccgccccc
    20401 gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga agctgaaaac
    20461 tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac ccagcaacaa
    20521 aaggacgccg ctcgtcagcg ttatcagtcg gcctcgcctg agcagcgcca ggcagtccgc
    20581 gagaaaatgc agactaaccc acagatccag cagcgaagag acgcagcgcg tgagcgtatt
    20641 cagtcagcct cgcctgagca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg
    20701 aaccaacagc aacgtgataa cgcccgccag cgtgttcaat cagcatcacc tgaacaacgt
    20761 caggtttttc gggaaaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat
    20821 actgtcaggc tgaataacga gcaacggtca gcagtacgcg aacgtctctc tgagcgcgga
    20881 gcaaggcgac aggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt
    20941 ttaattgccg gatgttccgg caaacgaaca attacttctt cttcgctttc gggttcggca
    21001 ggtcggtaat gctaccttcg aacacttctg ccgccaggcc cacagactcg tgcagagtcg
    21061 ggtgcgcgtg gatggtcagt gcgatgtctt cagcatcaca acccatttcg attgccaggc
    21121 cgatttcacc cagcagctcg ccgccgttgg taccgacaat cgcaccaccg atcacacggt
    21181 gagattcttt gtcgaaaatc agcttggtca taccgtctgc gcagtcggaa gcgatagcac
    21241 gaccagaagc agcccacggg aaggtggcgg tttcatagct gatgcctttc tctttcgctt
    21301 ctttctcagt cagacctacc catgcaactt ctggttcggt ataggcgatg gacgggataa
    21361 ctttcggatc gaagtagtgt ttcttaccgg cgataacttc agcggcaacg tgaccttcgt
    21421 gaacaccttt gtgtgccagc atcggctgac cgacgatatc gccgatagca aagatgtgcg
    21481 gtacgttggt acgcagctgt ttgtcaacgc ggatgaaacc acggtcgtcc acttccacgc
    21541 cagctttgcc tgcgtcgagg tttttaccgt tcggcacacg accaatcgct accagcacgg
    21601 cgtcgtaacg ctgcggttca gcgggtgctt ttttgccttc catcgtcaca taaataccgt
    21661 cttctttcgc ttcaacggcg gtaactttgg tttccagcat caggttgaat ttcttgctga
    21721 tacgcttggt gaagacttta acgatgtctt tgtcagctgc cgggataacc tggtcgaaca
    21781 tttcaaccac gtcaatctgt gaacccagcg cgtggtatac ggtgcccatt tccagaccga
    21841 tgataccgcc acccattacc agcaggcgtt ctggtacttc tttcagttcc agcgcgtcag
    21901 tggagtccca gatacgcgga tcttcatgcg gaataaacgg cagttggatc gggcgagaac
    21961 ccgctgcaat gatcgcattg tcgaagttga tcacggtttt gccgttctca ccttcaactt
    22021 ccagggtgtt agccccggtg aatttaccca gaccgttgac cactttgact ttgcggcctt
    22081 tcgccatacc agccagacca ccggtcagct gattgatcac tttctctttc caggtacgaa
    22141 tcttgtcaat atcggttttc ggttcgccga agacgatacc gtgttcagcc agcgctttgg
    22201 cttcttcgat aacttttgct acgtgcagca gtgctttaga agggatacag ccgacgttca
    22261 ggcaaacacc gccaagggtg ttgtaacgtt ctacgattac ggtttccaga cctaaatcag
    22321 cgcaacggaa ggcagcggag taacctgcgg ggcctgcccc aagtaccacg acctgagttt
    22381 tgatttcagt actcatcatg acctctatat atttatctcc ggcggtcata cccgtcgtct
    22441 ttcaggccgc agcggcgtta gcttcacgcc ctcaccccag tcacttactt atgtaagctc
    22501 ctggggattc tggcgcttgc taccttgctg cacccagaaa tccatagggt atgtgaagta
    22561 ataccctaac caccaccggg tcgttctatc cgtcggtatt ttacaaaatt gttaacaatt
    22621 tttaaacaac aaacggcaac cgatttgtct attcgctaat aacctcaata cattcatgag
    22681 attaccagaa aaaagccggc cgttgggccg gctcttttac ttacatcacc agacggcgaa
    22741 tgtcagacag cgtgttgtta atgatggtaa tgaaacgggc accatcagca ccgtcgatca
    22801 cgcggtggtc gaaggagaga gaaatcggca gcatcagacg cggcacgaac tctttaccat
    22861 tccacaccgg ctccatcgcg gacttggaaa cgccgaggat agccacttcc ggcgcgttca
    22921 caatcggcgc gaagtgggta gtacccaggc cgccgatgct ggagatggtg aagcaaccgc
    22981 cctgcatttc gcccgcagtc agcttaccgt cacgcgcttt cttagaaata gtcatcagct
    23041 cgcgagacag ctcgatgatg cctttcttgt tgacgtcttt gaataccgga acaaccagac
    23101 cgttcggggt atccaccgcc acaccgatgt tgatgtattt cttcagggtc agacgctgac
    23161 cgtcttccga caacgaactg ttgaagcgag gcatctgctc aagagctgca gcaacggctt
    23221 tcatgatgaa gacaaccggg gtgatcttca catccagctt acgtttcgcc gcttcttcgt
    23281 tctgctgttt acggaacgct tccagctcgg tgatatcggt tttgtcgaag tgagtaacat
    23341 gcgggatcat cacccagtta cggctcaggt tcgcaccaga aattttctgg atacggccca
    23401 gttccacttc ttcgatttca ccaaacttgc tgaagtccac cttcggccac ggcagcatgc
    23461 ctgggatacc gccgccagtc gccgccggag ctgcttctgc acgtttgata gcttctttca
    23521 cgtaagcctg aacgtcttcg cgcaggatac gacctttacg gccagtgccc ttcactttcg
    23581 ccaggttaac gccaaactcg cgtgccagac ggcggatcag cggagtcgcg tgaacgtaag
    23641 cgtcgttttc tgcaaattca gatttgcctt ccgctttcgc agccggtgct gctgccggag
    23701 cttcagcttt tgctgccgga gccggcgctg ccgcttcctg tttcgcagga gctgccgcag
    23761 gcgctgcgcc ttcaacttcg aagatcataa tcagcgagcc agttttcact ttatcgccaa
    23821 cgttgacttt cagttccttc acgacgcctg caaacggagc cggaacttcc atagaagctt
    23881 tgtcgccttc tacggtaatc agtgactgtt cagcggcaac tttgtcgccc actttcacca
    23941 tcacttcggt cacttcaact tcgtcaccgc cgatatccgg aacgttaact tctttcacgc
    24001 cagccgctgg tgcaggggcc gctgccggag ccgcttcctg tttagccgcc ggagctgccg
    24061 cgcctgcttc acccgcgact tcgaagacca taatcagcga gccggtagac actttgtcac
    24121 ccacgttcac tttgatctct ttcacggtgc cagcaaacgg agccggaact tccatagacg
    24181 ctttatcgcc ttctacggtg atcagcgact gttcagcttc aactttatcg ccaactttca
    24241 ccaggatttc ggtcacttca acttcgtcgc tgccgatatc cggaacgtta acgtcttttg
    24301 ccgctgcagc cgctggtgct gctgccggag ctgtttcttt cttctcttct gcctgagcag
    24361 gtgcagcgtc tggtgcaccg tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg
    24421 ttttatcgcc aacagagact ttgatctctt taacgatacc cgcctgcgga gacggaactt
    24481 ccatagaggc tttgtcgcct tctacggtga tcagcgactg ttcggcttca actttgtcgc
    24541 ccactttgac caggatctcg gtgatttcaa cttcatcagc cccgatgtcc ggtactttga
    24601 tttcgatagc cattattctt ttacctctta cgccagacgc gggttaactt tatctgcatc
    24661 gatgttgaat ttggcgattg cgtcagcaac cactttctta tcgatttcgc cacgtttagc
    24721 cagttcgccc agcgccgcta caaccacgta ggaagcatca acttcgaagt ggtgacgcag
    24781 gttctcacgg ctgtcggaac gaccgaagcc atcagtaccc agtacgcggt agtcgtcagc
    24841 cggtacgtaa gtacggacct gctcagcgaa cagtttcata tagtcggtag atgccactgc
    24901 cggagcgtcg ttcatcacct gagcgatata cggaacgcgc ggagtttcca gcgggtgcag
    24961 catgttccag cgttcacaat cctgaccatc tcgcgccagt tcggtgaagg aggtcacgct
    25021 ataaacgtca gaacctacgc cgtaatcttt cgccaggatc tctgccgctt cacggacgtg
    25081 acgcaggatg gaaccggagc ccagcagctg aactttacct ttgctacctt caatggtttc
    25141 gagtttgtag atacctttac ggataccttc ctcagcacct tccggcattg ccggcatgtg
    25201 gtagttttcg ttcagcgtag tgatgtagta gtaaacgttc tcttgttttt caccgtacat
    25261 acgctccaga ccgtcatgca taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga
    25321 gatacagttc gggatagtca gcgactgaat gtggctgtga ccatcttcgt gctgcagacc
    25381 ttcaccgttc agggtggtac gaccggaagt accgccgatc aggaagccac gcgcttgctg
    25441 gtcgccagcc gcccagcaca gatcgccgat acgctggaag ccgaacatcg agtaatagat
    25501 gtagaacgga atcatcggca gattgttggt gctgtaagag gtcgccgctg ccagccagga
    25561 acaacctgcg cccaactcgt tgatcccttc ctgcagaatc tgacctttct cgtcttcttt
    25621 atagtaagca acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat
    25681 accaatctga cggaacagac cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg
    25741 taccagacga tctttgatcg acttgttctt cagcattacg ttcagggcac gaacgaaagc
    25801 gatagtggta gagatctctt tgctctgctc ttccagcagc gcgccgaagt cttgcaggct
    25861 cggcagctca agcttctcgg tgaagttcgg ctgacggctt ggcagataac cgtgcagttt
    25921 ctgacgctgt gcgtgcagat aggtatgctc ttcagaacct tccgggaagg tgatgtacgg
    25981 cagtttttcg atatctgcat cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac
    26041 gccgtccatg ttcattttct taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc
    26101 catgccgtaa cctttaatgg tatgagcaag gattactgtc gctttgcctt tggtttcctg
    26161 cgctttcttg aatgcagcgt agattttctt cggatcgtga ccaccacggt tcagtgccca
    26221 gatctgttcg tcagtccagt ctgcaaccag tgctgcggtt tccggatatt taccgaagaa
    26281 gtgttcacga acgtacgcac catctttcga tttgaaggtc tggtagtcgc cgtcaacggt
    26341 ttcgttcatc agctggatca gtttaccgct ggtatcttta cgcagcagtt catcccaacg
    26401 gctaccccac atcactttga tcacgttcca gccagcacct tcgaagatgc cttccagttc
    26461 gttgatgatc ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat
    26521 aacgaagacc aggttatcca gtttttcacg ggtagcgatg gtgatcgcac ctttggattc
    26581 cggttcgtcc atttcaccgt cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt
    26641 caggccacgg tgttccagat atttcaggaa tttagcctgg taaatagcac caatcggccc
    26701 cagacccata gatacggtcg ggaactgcca gaattccggc atcagtttcg ggtgcggata
    26761 ggaagagagg ccattgccgt gaacttcctg acggaagtta tccagctgct cctgagtcag
    26821 acgaccttcc aggaaagcac gagcgtacac gcccggggag atgtggccct ggaagtaaac
    26881 caggtcgccg ccatcctgct cgttgcgtgc acggaagaag tggttaaagc acacatcata
    26941 aatggttgcg gaagactgga aggacgccat gtggccgccc agttcgagat cttttttcga
    27001 cgcacgcagc accgtcatga tggcgttcca gcgaatagct gaacgaatac ggcgttccag
    27061 ttccagatta cccggatact ccggttgttc ttcaacgggg atggtgttga tgtagttgct
    27121 gatacctgtg cctgcggcta cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc
    27181 gatcagatac tgagcacgct caacaccttc ttcacggatg accgattcga tcgcctggag
    27241 ccagtcgcga gtttcgatcg gatccacgtc atttgggaaa cgttctgaca tgggttattc
    27301 cttatctatc taataacgtt gagttttctg gaacctgtcc cattgaactc tcgccggaaa
    27361 gctcaataag acaggttcta cgtttagttg ccgcgctttt atatgcgctt gatttacaac
    27421 atcttctgga taatttttac cagaaaaatc actaattctt tcgttgctcc agacgacgca
    27481 gagaacgctc acggcggctc tcttcacgac ttctgtcgag caaaatttct tcgataaagg
    27541 ccagatggcg atgcgatgct tcgcgcgctt cttccggctt accggccata atcgcttcaa
    27601 atatgcgggt gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt
    27661 cgaagttctg gcggacattc tgggccaaca tcggctccat acaccttagc agatgaagca
    27721 gaaccacatt gtgggccgct tcggtgacgg caatctgata ctggagtacg gcgtttgatt
    27781 ccgcgtccag atcgccagac tgctgcgcca gctctatggc gtggtggagt tcacggatgc
    27841 gttccttgtc ttcatcggta ctacgcagcg cggcgtaata agcggcgata ccttccaggg
    27901 cgtgtcgtgt ttcgagcaag tcatactgtg actcaggatg gtcggagagc agctccacca
    27961 gcggatcgct gaagctttgc cataggctgc tctggacaaa agtgccgcca ccctggcgac
    28021 gaagcaacaa gcccttcgct tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga
    28081 cgtcaaactg ttttgccagt tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc
    28141 cttcgaggat caaaaactcc agttgctgct caatcacatc ggagagtttt ggttggcgga
    28201 ttttgctgta ggccatgagt tcctgtctta agccacttgc cgaagtcaat tggtcttacc
    28261 aatttcatgt ctgtgacgct aaagtaacaa agtattcacc ttatgtccat acaggttttg
    28321 attgaaatca tgaaactgtg cacattttaa caacttgaca tatataacgt ttcaaagttg
    28381 taactatgca caaatgtaga ctttacgtag gaaaggagtt ttttaacgat tattaaacta
    28441 taaaaatccg aattgaaccg attcacttac caattttgtg atttttaatt caattaaaac
    28501 gaatttaaat tcattctaca tattgagagg ggttgaggct gagctttaca aacggtttct
    28561 ttttaagcaa ctcatcttca accatgcata aagcgggtgc attcgctgcc gcataccatt
    28621 attcttgatc tgacggaagt ctttttgtag caattcaaac ttctttgatg taaacaaatt
    28681 aatacaacaa acggaattgc aaacttacac acgcatcact gcgtagatca aaaaaacaac
    28741 caccgcacga ggtttcatga tggaaggtca acagcacggc gagcagctaa agcgcggcct
    28801 taaaaaccgc catattcagc ttatcgcgct gggtggcgcg atagggaccg ggttattcct
    28861 gggtagcgcc tccgtaatac agtccgcagg gccagggatt atcctgggtt acgccattgc
    28921 tggttttatc gcctttctga tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt
    28981 cgcaggctcc tttagccact ttgcttataa atactggggc agttttgccg gtttcgcctc
    29041 tggctggaac tactgggtac tgtacgtttt agttgccatg gctgagctga ctgccgtggg
    29101 taaatacatt cagttctggt atccggaaat ccccacctgg gtttctgccg ccgtattctt
    29161 tgtggtgatt aacgccatca acctgaccaa cgttaaagtg tttggcgaga tggagttctg
    29221 gtttgccatt atcaaagtta tcgcggtggt agcgatgatc atcttcggcg gctggctgct
    29281 attcagtggc aacggcggcc cgcaggcgac cgttagcaac ctgtgggatc agggtggttt
    29341 cctgccgcac ggcttcaccg ggctggtgat gatgatggcg attatcatgt tctcgttcgg
    29401 tggtctggaa ctggtgggga tcaccgcagc agaagctgat aacccggagc aaagtatacc
    29461 gaaagcaact aaccaggtta tctaccgcat cctgattttc tatattggtt cgttagccgt
    29521 tctgctctca ctgatgccgt ggacccgcgt taccgccgat accagtccgt ttgtgctgat
    29581 cttccacgag ttaggcgata cctttgtggc gaatgcgctg aacatcgtgg tactgactgc
    29641 ggcgctctcc gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc
    29701 acaacagggt aatgcgccaa aagcgctggc gtctgtcgat aaacgtggtg taccagtaaa
    29761 taccattctg gtgtctgcac tggtaacggc gttgtgcgta ctgattaact accttgcccc
    29821 agagtccgct ttcggactgt taatggcgct ggtggtatct gcactggtaa tcaactgggc
    29881 gatgattagc ctggcgcata tgaaattccg tcgcgccaag caggaacaag gcgtggtaac
    29941 tcacttccct gctctgcttt atccgctggg taactggatc tgcctgctgt ttatggcggc
    30001 ggtactggtg attatgctga tgaccccagg aatggcgatt tcggtatacc tgatcccggt
    30061 atggctgatc gtgttaggta tcggctatct gtttaaagag aaaaccgcca aagccgtaaa
    30121 agcgcattaa tctctctacg ccctcacccg tacagggtga gggcaataat ctttacacca
    30181 acgccccgta aatcgtcagt agtgcaatca ccaccacgac cacgaacgag gttttcttcg
    30241 ccattgaaac cgctgccttc ggcgtctcca ccttatcgac atgcggttca cgcgccagag
    30301 agaactgcgc cagacgcgtt aacacctgat actgcgaagt atggaaatca cccagcgaag
    30361 caaaccaggc tggtaacgct ttctcaccat gaccgatcaa ggcatatacc acacccgcaa
    30421 gacgaaccgg cacccaatcc agtacatgaa gcacggcatc aatgccggac tgtaaacgat
    30481 gatgcggcgt ctgatatcgc gccagccagt attgccatgc acgcaaaaac gcatacccca
    30541 tcagcgtaac gggtccccag gttcccccca caatcagcca gaacagcggt gcaagataaa
    30601 aacgaaagtt aatccacagc aatgcatttt gcagctcacg caaatactca cgttcgtcgc
    30661 agcctgccgg gacgccgtga atcatggtga gttcgccagc catcgtggca cgggcatggc
    30721 tatcattacg tgaagcagct gtcagataag catgataatg aagacgaact ttacctgcgc
    30781 caatacacag caaaccaatc agcagccaca ccagtagcgt gggaacgttg aacaatactc
    30841 cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc aataatggtc atgcctaacg
    30901 tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct
    30961 gccagtgctc gcccaactta aacaggcgct cgaaaattaa caccagtaag gttgtaaata
    31021 gcgtcatgtt gtctccttgc tgaccagcac acgaaaccgt gcccaatcaa atgcaggacc
    31081 gggatcggtt ttccgatccg gcgcaatatc acaatggccc gtcatgtttt tagcgatatc
    31141 cggatagcaa tcaatcagtg cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt
    31201 atacgccagc gtatcggtgc cttcaagctc aatcccaata gaaaaatcat tgcagcgttc
    31261 gcgcccctga tactgagaga ctcccgcatg ccatgcacgt ttatcgaaag gaacatactg
    31321 gactatttca ccatcacggc gaatcaaaca gtgagcggag acgcgcaaat gggcgatttc
    31381 agcaaagaaa ggatgtgcct gcggatcaat agttccagtg aataatgcgt cgatccacgg
    31441 accgccaaac tcgcctggcg gcaggctaat attgtgcacc accagcaggg tgggtgtttc
    31501 gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc
    31561 ctgttctaac aacatgcagg atctccttat atgtggtgct aatacccggt tcagagtagc
    31621 atgtttctac cttatgattc gttagctatc tggagtttta acatgccgcc tcgccgctat
    31681 aaccctgaca cccgacgtga cgagctgctg gaacgcatta atctcgatat ccccggcgcg
    31741 gtggcccagg cgctgcggga agatttaggc ggaacagtcg atgccaacaa tgatattacg
    31801 gcaaaacttt taccggaaaa ttctcgctct catgccacgg tgatcacccg cgagaatggc
    31861 gtcttttgcg gcaaacgctg ggttgaagag gtgtttattc aactggcagg cgacgatgtc
    31921 accataatct ggcatgtgga tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa
    31981 cttgaaggcc catcccgcgt gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa
    32041 accctttcag gagttgccag taaggtacgc cactatgtcg aattgctgga aggcaccaac
    32101 acgcagttgt tggatacgcg caaaacctta cccggcctgc gttcagctct gaaatacgcg
    32161 gttctttgcg gcggcggagc gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa
    32221 gaaaaccata ttattgcctc cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg
    32281 cacccggatg cgccagtaga agtcgaagta gagaatctgg aagaacttga tgaagccctg
    32341 aaagcaggag ccgatatcat catgctggat aacttcgaaa cagaacagat gcgcgaagcc
    32401 gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca
    32461 ctgcgtgaat ttgccgaaac gggcgtggac tttatctccg tcggtgcgct aactaaacac
    32521 gtacaagcac tcgacctttc aatgcgtttt cgctaatagt tgacagattt atccgctcca
    32581 tcgcgggcgg ataatctccc cttccccaac tttcttcgta acgcctcgca aatttcttgt
    32641 ctttcgctgc aacgaaataa aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc
    32701 tcgcctgtgc tgatggcgtt tgccaaagta gcgccaacta aatcaaggag cgaaacagat
    32761 ggacaagcaa cgcggtttta cacttatcga actgatggtg gttattggca tcattgccat
    32821 tttaagcgcc attggtattc ccgcttatca aaactacctg cgcaaagccg cactcaccga
    32881 catgctacaa acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg
    32941 tggattagat acctgcgacg gtggcagcaa tggcattccc tcgcctacca ccacccgcta
    33001 tgtttcagcc atgagtgtgg caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa
    33061 tgggctaagc gtcgtcatga caccgggttg ggataacgca aacggcgtca ccggctggac
    33121 gcgcaactgc aatattcaaa gtgacagcgc attgcatcaa gcctgcgaag atgtcttccg
    33181 ctttgatgac gccaactaag gagcggcaat gaatattcca cagctcactg ccctgtgtct
    33241 gcgttatcat ggagtcttgc tggatgccag cgaagaggtg gttcatgttg cggtagtcga
    33301 tgcaccttcg catgagctac tggacgcatt gcatttcgct accaccaaac gtattgagat
    33361 cacctgctgg acgcgccaac aaatggaagg tcacgccagt cgcacacaac agacattgcc
    33421 cgtagctgtt caggagaagc atcagcccaa agcagagttg ctaactcgaa cgttacaatc
    33481 tgcgctggaa caacgcgcgt ctgatattca tatcgaacca gcggacaatg cctaccgcat
    33541 ccgcttgcgt atcgacggcg tattgcatcc tttaccggat gtttcaccgg atgccggagt
    33601 cgcattaacc gccagattaa aagtgctggg aaacctggat attgcggaac atcgcctgcc
    33661 gcaggacggg caattcactg tcgaactggc aggaaacgcc gtctcatttc gtattgcgac
    33721 cttaccatgt cggggtggtg aaaaggtggt attaaggttg ttacagcagg tgagccaggc
    33781 actggatgtc aacacgcttg gaatgcagcc gttacaactg gcggactttg ctcatgcctt
    33841 gcaacaacca cagggactgg tgctggtaac tggccctaca ggcagcggca aaacggtcac
    33901 gctttatagt gccctgcaaa cgctgaatac cgctgacatt aatatttgta gcgtcgaaga
    33961 tccggttgag atccccatag ccggactaaa ccagacgcaa atccatccgc gtgccggact
    34021 cacctttcag ggcgtgttgc gtgcgttatt gcgccaggat cctgacgtca tcatgatcgg
    34081 agagatccgc gatggcgaaa cagcagagat cgctattaaa gcggcgcaaa ctggtcacct
    34141 ggtgttgtct accctacaca ctaattccac ctgcgaaacg ctggtacgtt tacagcaaat
    34201 gggggtcgcc cgctggatgc tatcatcggc gcttacgctg gtaatagccc agcgtctggt
    34261 acgcaaactt tgcccacatt gtcgccggca gcaaggggag cccatccaca ttccagacaa
    34321 tgtatggcca tcgccactgc cccactggca ggcacccggt tgtgtacatt gctaccacgg
    34381 tttttatggt cgtacggcct tatttgaagt tctgcccata acgccggtca ttcgtcagct
    34441 tatttccgct aataccgacg ttgaatcgct ggaaacgcac gcacgacagg cgggtatgcg
    34501 tacgcttttt gaaaacggct gcctggccgt ggagcaaggc ttaaccacct ttgaagagtt
    34561 aatccgcgta ctggggatgc cgcatggcga gtaagcaact ctggcgctgg catggcataa
    34621 ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga aagccgtgct ttgctgctca
    34681 tggcactaca gcaacagatg gttaccccac ttagcctgaa gcgaatcgcc atcaattctg
    34741 cgcagtggcg aggagataaa agcgcggaag tcattcatca actggcgacg ctactcaaag
    34801 ccgggttaac gctttctgaa gggctggcac tgctggcgga acagcatccc agtaagcaat
    34861 ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca gggcattgct ttttccaatg
    34921 ccttattacc ctggtcagag gtatttccgc cactctatca ggcgatgatc cgcacgggtg
    34981 aactgaccgg taagctggat gaatgctgct ttgaactggc gcgtcagcaa aaagcccagc
    35041 gtcagttgac cgacaaagtg aaatcagcgt tacgttatcc catcatcatt ttagcgatgg
    35101 caatcatggt ggttgtggca atgctgcatt ttgttctgcc ggagtttgcc gctatctata
    35161 agaccttcaa caccccacta ccggcactaa cgcaggggat catgacgctg gcagacttta
    35221 gtggcgaatg gagctggctg ctggtgttgt tcggctttct gctggcgata gccaataagt
    35281 tgctgatgcg ccgaccgacc tggcttatag tgcggcagaa attgctgtta cgaatcccga
    35341 ttatgggttc actgatgcgg ggacaaaaac tcacgcagat ctttacgatt ctggcgctga
    35401 cacaaagtgc aggcattact tttttgcagg gcgtagagag cgtcagagaa acaatgcgct
    35461 gcccgtactg ggtgcaactt ctgacacaaa tccagcacga tatcagtaac ggtcatccca
    35521 tctggctggc gctaaaaaat accggggagt ttagcccgct ctgtttgcaa ttagtgagaa
    35581 caggagaggc atccggctcg ctggacctca tgttagacaa cctcgcccat catcatcggg
    35641 ataacacaat ggcgctggcg gataacctcg cagccttact ggaaccggcg ttgctgatca
    35701 taacgggagg aattatcggt acgctggtgg tggcgatgta tctgccaatt ttccatttag
    35761 gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc tccacaccag cgttggaaga
    35821 ttacaggttg ttgaagatgc ggttttcttg ttcctgcaca cggataaatg tggtgcgctt
    35881 ggtcagttct ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca ggccgcccaa
    35941 aatatcacgc gcggtatttt caaccgggcc tcgcagcggc agcttaacgg ttttaccttc
    36001 tgctgcgcga tattccgcaa cgccgccaac gtgacgtttc atcgcagact cggagctcat
    36061 gccgtagaac agcataaatt tctcgccgtt ctcctcaacg atgcgaccgc cgctctcttc
    36121 gtggcccgcc agcatgccgc caagcatgac gaaatcggca ccgccgccaa aggcttttgc
    36181 gacatcgccc ggcgtggtgc agccaccgtc gctgacgatc attccgccca gaccgtgcgc
    36241 cgcatcggca cattcgatta ccgcagaaag ctgcggataa ccgacgcctg ttttgacgcg
    36301 agttgtgcaa acagaacctg ggccaatgcc aactttaacg atatcagcac cggagaggat
    36361 aagctcctca cacatttcac cagtcactac gttgccagca caaatggttt tggtcggcca
    36421 cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg gaataaccat tcgccacatc
    36481 aatacaaacg aagtttaatg ccgggttcag gtcgagaatc tgtttagttt tttcgaaatc
    36541 cgcatcagac gtaccggtag aaaccatcac atgtttcagc acatcagcgg aagaattgtt
    36601 gataaacgct tgccactctt cgacagaata gtgtttatgc acagcagtca aaatatcaaa
    36661 agaagccagc gcagaggcca tagaaaatgt acctacggtg tccatatttg cggcgataat
    36721 tggcacgccg gaccagctct gacctgaatg tttgaaggtg aattgacgtt ccagttcaac
    36781 atcggaacgg cttttaagag tggagcgttt agggcggatg agaacgtctt taaaacctaa
    36841 cttcagatct tcttcaatac gcatgtgcgg attcctgggg ttaatggcta taagggtaag
    36901 agcgcaactc cagtgacgct atcatacgca ctaatcaatg cggcgcaaga ctgcgaaatt
    36961 gtcttttttt acgctacaat cccataaatt tacctggtaa atagtgagcg aacgtttcat
    37021 caatttcgct ttcgtgcact catttttaac gttttgataa tcatgatgaa ttcccgggaa
    37081 tataaattat gaggtatata gttgccttaa cgggaggcat tggcagtggc aagagtaccg
    37141 ttgccaatgc gtttgctgat ctcggaatta acgtcattga tgccgatatt attgcgcgtc
    37201 aggtggttga accaggtgca cctgcgctac atgccattgc tgatcacttt ggcgctaaca
    37261 tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc ttcgccaacc
    37321 cggaagagaa aaactggctt aacgccctgc tgcatccgct gattcagcaa gagacgcaac
    37381 accagatcca gcaagctact tccccctatg tactgtgggt tgtgccattg ctggtagaaa
    37441 actcactgta taaaaaagcg aatcgagtgc tggtggtgga tgtcagccca gaaacgcaac
    37501 ttaagcgcac catgcagcgc gatgatgtaa ctcgcgagca tgtcgaacaa atccttgctg
    37561 ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga cgtcattgat aataacggcg
    37621 caccggatgc tatcgcatcg gatgttgccc gcctgcacgc acactatttg cagcttgcgt
    37681 cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag gtcctttttg aacatccact
    37741 aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg attcagcaac tcaccgttaa
    37801 tttacccatc gttgaccacg ctggcgcgct gcatttcttc cgtaatgtca gtgaattact
    37861 ggatgttttc gagcgcggcg aagtccgcac tgagctgttg aaagaacttg accggcagca
    37921 acgtaaactc cagacctgga ttggcgtgcc tggcgtggac cagagccgta ttgaagcatt
    37981 aattcagcag ttaaaagcgg cggggagcgt attaatttcc gcgccgcgta tcgggcaatt
    38041 tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga ctgagcatcc caggcggctg
    38101 ttgcagcttt gatttaccta cattgcacat ttggctgcat ctaccccagg cgcagcgcga
    38161 cagccaggta gaaacctgga ttgccagcct gaacccgctc acccaggcac ttaccatggt
    38221 gctggattta attcgccagt cggctccctt ccgtaaacaa accagcctga atggttttta
    38281 tcaggataac ggtggcgatg ccgacttgct gcgcctgaat ctgtcgctcg attcacagct
    38341 ttatccgcaa atttccggac ataagagccg ttttgccatt cgttttatgc cgctggacac
    38401 tgaaaacgga caggtaccgg aacgtctgga tttcgaactg gcctgttgct aaggagtgaa
    38461 aatgtcagaa actattacgg tgaattgccc aacctgcggg aaaacggtgg tgtggggtga
    38521 aatcagcccg tttcggccat tttgctccaa acgttgtcag ctgatcgacc tcggagaatg
    38581 ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt tccgaaagcg atgactggag
    38641 cgaagagcca aagcagtgac atttgtctga tgccgcacgt aggcctgata agacgcggac
    38701 agcgtcgcat caggcatctt gtgcaaatgt cggatgcggc gtgaacgcct tatccgacct
    38761 acagacgctt aagcttcgca attaccggtt cattggctgg cggaaaatca tcggcattaa
    38821 gaccgactag cgacatccac tcaccgggtt gcccttcttt accccacggc tccccttccc
    38881 agctttcgac cagccagaac cacagagtta tatgcctgtc cgggaattca tattccagtt
    38941 tttcaaatag cgaaaaatgt tgtggggtaa tcccgacttc ttcctgaagt tcacgcacca
    39001 ccgcctgttc cggcgtttcg cccatttcaa ttttaccgcc gggaaactcc agtttattcg
    39061 ccatgtgcgc atctgctgcg cgacgcgtta taaagatttc attgttctcg ttgcgaataa
    39121 tacctaccgc aatttgcagc tttttcattg tcttaaacct ataaaaaagg cgcagaatcc
    39181 tgcgcctttt acttcaactg ttagctttta ttgcaggcga ccatggcact gcttgtattt
    39241 tttaccagaa ccgcacgggc aaggatcgtt acgtcctact ttgcgctcac cggtttgcgc
    39301 cgccagtgca gctgcggctg cagagtcgtc atcctgatgg ctaagctgct gcatttgcgc
    39361 taaacgctcg gcttccatac gacgctgttg ttccagctcc tcaacctctt caggcatacg
    39421 tacctggact ttgctcagcg tgctgataac ttcatatttt aacgactcca gcatcgctgc
    39481 aaacatggag aacgattcac gtttgtattc ctgcttcgga tctttctgtg catagccacg
    39541 caggtggata ccctgacgca gatagtccat cgctgccagg tgctctttcc acagggagtc
    39601 aagcgtttgc agcatgacgc ctttctcgaa gtgacgcatc atctcagcac caaccacttc
    39661 ttctttacgc tgatacactt cgatggactg cgccagaatg cgctcacgca gcgtctcttc
    39721 atgcagttct ggttctttat ccagccactc ggcaattggt aaatcgaggt cgaaatcgtt
    39781 cttcagacgt tcctgcagcc ccggaatatc ccacatttct tccagcgact gtggcggaat
    39841 gtaggcatca atggtggctt tgaacacatc ttcacgaatg ctgttgatgg tttcgctcac
    39901 atcgctgaca tccagcagtt cgttacgctg ggagtaaatg gcgcgacgct gatcgttagc
    39961 cacgtcatca tattccagca gttgcttacg aatgtcgaag ttacggcttt caactttacg
    40021 ctgggcgttg gcaatcgctt tagtcaccca cgggtgttca atggcttcgc ctggcttcat
    40081 acccagttta cgcatcatgc cggatactcg gtcggaagca aaaatacgca tcagcgcatc
    40141 ttccatcgac aggtagaaac gggaagaacc agcatccccc tgacgaccag aacgaccgcg
    40201 caactggtta tcgatacgac gggattcgtg acgctcggta ccgatgatat gcaggccacc
    40261 tgcttccagt accgcatcgt gacgtacctg ccagtcggct ttaatttttt caatttgctc
    40321 tgcggtcgga ttttccagcg cggcaacttc tgcctgccag ctaccaccga gcacaatatc
    40381 tgtaccacga cccgccatat tggtcgcgat agtcaccgca gccggataac ctgcctgagc
    40441 aacaatcgcc gcttcgttgg cgtggaattt ggcgttcagg acgttgtgct taataccggc
    40501 tttggtcagt tcgtttgaca ccagctccga tttttcgatg gagatagtac ccaccagcac
    40561 cggctggcct ttcgcagtac gttctttgat atcttcaatg atcgcctgaa ttttttccgc
    40621 ttcagtcatg tagaccaggt ccggcagatc tttacgaatc attggacggt tggtcggaac
    40681 aacgacggta tccagcttgt agatggagct aaattcgaaa gcttcggtat cagcagtacc
    40741 ggtcatcccc gccagttttt catacagacg gaagtagttc tggaaggtga tcgaagccag
    40801 cgtttggttt tcgttctgga tctgcacacc ttctttcgct tccacagcct ggtgcagacc
    40861 atcggaccag cgacggccct gcatggtacg accggtgtgt tcgtcaacga tgataacttc
    40921 accatcttta acgatgtagt cgacgtcgcg ggtaaacagc gcatgagcgc gcagcgccgc
    40981 cgttacgtgg tgcatcagca tgatgttggc cggagagtac agagactccc cttcatccat
    41041 gatgccctct ttcaccagca gttcttcaat cagcaccaga ccacgttcgg tcaggttcac
    41101 ctggcgagat ttttcgtcca ccgagaagtg gccttcgccc tggaaggttt cggagtcttc
    41161 tttttcctga cggatcaggt gcggaataat tttattcacg cgtttataca tttccgagct
    41221 gtcttctgcc gggccggaaa tgatcagcgg tgtacgcgct tcatcgatca ggatggagtc
    41281 cacttcgtcc accagcgcat agtgcagttt acgctgtaca cgttcttcag ggctgaacgc
    41341 catattgtcg cgcaggtagt caaagccgta ttcgttgttc gtaccgtaag tgatgtcagc
    41401 tgcgtaagct tcgcgctttg ccggtgctgg catgcccggc aggttgatac cgacagtcag
    41461 gccaaggaat tcaaacagcg gacggttgtt ttcggcgtca cgttgcgcca ggtagtcgtt
    41521 gacggtaact acgtgcacgc ctttaccggt tagtgcgttc aggtaagcag gcagcgttgc
    41581 ggtcagggtt ttaccttcac cggtacgcat ttcggcgatg cagcgttcgt taagaaccat
    41641 accgccgagt aactgaacgt cgaagtgacg cataccaaag acgcgcttac ttgcctcacg
    41701 taccacggcg aaagcttccg ggatcagatt ttccagcact tcgccttttt ccagacgtgc
    41761 acgaaactct gcggttttcc ctttcagttc ttcgtcggag agtttttcca tctccggttc
    41821 catggcattg atgatgttga ccactttgcg catccggcgc agggtgcgat cgttacgact
    41881 accgaaaact ttagttaaca atttgattag cataataaaa tctcaaacgc cccgcgttgc
    41941 ggagttaata aaatgaagta aaggtttatt gttgttaggt gaggcgttga gggccagcac
    42001 ggatgccttg cgcctggctt atccagacgg gcgtgctgaa ttttgcttgt ggggtaaaat
    42061 gcgcataatc aatgcgataa cccttttcag acggcgtgcc ttcctgggtc agcagcgcgc
    42121 tgagcgtatc cagtaatgca agatgttgcg cctgaagagg caaagattct tcagcaacgg
    42181 gcagtgtttg cggtgccatt gcgaaagaaa gatgacggat taccgtgcga atggcatgtt
    42241 gatgccagta atcaacggaa tagttcgaat tcgggcggcg tgtgttcgct tccagcaagg
    42301 ccaattgacc aaagttaact ttggctgaag gctcgtggtt gcgggttgtc gcttttgcgg
    42361 gcgcgtttgg ttcggcggcg ttgctgagcg caggcaaacc taaactcgcc gcaaccatcc
    42421 ctaataagag atgcggccag aagtagcgtt taccaaactg tcgccagcgc gtcagtattc
    42481 cactcacgtt attgccatcc cgatcgtatt caaacgtatt tagcgcacaa atcttatcat
    42541 taaagcctgc agtccacagc acgaatgaag ataagttgtg cggaaaagtg cttaagaaaa
    42601 cagcgttcgc accgtttttt atagctctta taaaattcaa aaaaaaacga ctggttcacc
    42661 tggccggaga gagtgccaga tttgccagtc gaattttata cgacagtata aatgtcgtta
    42721 tgccagtaca gctgaaggcg ctttgaaggc caacggcagt tctgcgtcgt cctggaaggt
    42781 cacatattcc caggcttcct gtttcgccag gacagcctgc agcagtttgt tattcagtgc
    42841 atgaccggat ttataagcgg taaatgcacc aataatattg tgaccacaca tgaacaagtc
    42901 accgatcgca tcgagcattt tgtgacgcac aaattcgtct tcaaaacgca ggccgtcttc
    42961 gttcagtacg cgataatcgt caacaacgat ggcacaatcg aagctgccgc ccaggcacaa
    43021 accacgggac tgcagatatt cgatatcacg catgaaaccg aacgtacgcg cacggctgat
    43081 ctggcgcata aacgcatcag cggagaagtt catcgcatag cgctggttgc tggaatcaat
    43141 agccggatgg ttaaaatcga tggtgaaatc cagcgaaaaa ccattgtacg gcttaaattc
    43201 agcccactta tcgccatctt cgacacgaac agtctctttg atgcgaacaa attttttggc
    43261 gcagttcaac tcgtcgatac cggcgtcaag cagcaggtat acaaacggag cggcgctgcc
    43321 gtccatgatc gggatttccg gcgcgttaac ttcgataaca atgttatcga tgcccaagcc
    43381 cgcgagagca gcattgaggt gctctacggt tgaaatccgt acatcatgct cgttgaccag
    43441 acacgtacag agcatggtat cacgcacaga tttggcatcg gccgggaaat ctaccggtgg
    43501 attcaagtcg gtgcgacgat agatgacccc ggtgttggcc ggcgcagggc gtaacgtcag
    43561 ggtgactttc ttgccggtat gtaaaccgac acccgtcgcc tgaacgatac gtttaagtgt
    43621 cctttgtttg atcatcgtat tatctcgcca aattacctat ccaaccgaag tgtactatac
    43681 attcggcggg ccagtttagc acaaagagcc tcgaaaccca aattccagtc aattcttaat
    43741 cagcttgctt acgcaggaat gctgggatat ccagataatc cggctctttc gcagtttgcg
    43801 gcgcattgtc attcacgact ttagcaaccg gcttctgctc ctgggtcagc ggagccatcc
    43861 catgctgctg gtagcgatcc atcactggct gctgaacctg cttattggtc accagagtga
    43921 tttcaggacg tttgtccatg ccgatacctg tcgcaacaac ggttacgcgc agctcgtcat
    43981 tcatatccgg gtcaagagaa gtaccgataa ccacagtcgc gttgtcggaa gcaaatgcac
    44041 ggatggtgtt acctaccgtt tcgaactcat ccagacgcag gtcgaagccc gccgtgatgt
    44101 taaccagcac gccgcgcgcg ccagacaggt cgatatcttc cagcagcgga gaagagatag
    44161 ccatttcagc agcttcttcc gcacggtctt caccgctcgc cacgccagaa cccatcattg
    44221 cgtagcccat ctcagacatt acggtgcgta cgtctgcaaa gtccacgttc atcaaacccg
    44281 gacgagtaat cagttcagcg ataccttgca cagcgccttt cagtacatcg ttcgctgcgc
    44341 caaacgcatc cagcagggag ataccgcggc ccagaacttt cagcagtttg tcgttcggga
    44401 tagtgatcag agagtccaca tgcttggaca gttcagtgat cccctgctcc gcgaatgcca
    44461 tacgcttctt gccttcaaag ttgaaaggct tagtgacgac agcaacggtc aggataccca
    44521 aatcttttgc cacttcagcg acgactggtg ctgcacctgt accggtacca ccacccatac
    44581 ccgcagcaat aaagaccatg tctgcacctt ccagcgccgc acgcaatgca tcgcgatcct
    44641 catcagccgc attgcggcca acttctggat tagcgccagc gcccagtcct ttggtgatac
    44701 cgctaccgat ttgaatcgtc tgtccaaccg ctgttttacg cagcgcttgt gcatcggtat
    44761 ttaccgcgaa gaattcaaca ccttcaatgc gctcgcgcac catgtgttca acagcattac
    44821 cgccgccgcc gccgacgccg atgactttaa tcaccgcgtc attggtaagt tccattggtt
    44881 caaacatagt ttctctccga tttgtgcctg tcgcctgagg ccgtaatcat cgtcggcctc
    44941 ataaaaatta aaactctttt cgcagccaac tattgagtcg cttgatccac gagccaactg
    45001 atgctgtaac acgtttttct acttcagctt caccgttaag atgtgactct ttcccatagt
    45061 gaagcaatcc caccgccgtc gaataatacg gctcctgagc ataatccgtt aaaccggtaa
    45121 tgttcagcgg cgcgccgata cgcacttgcg tatgaaacac gcgctgagca caggctgcaa
    45181 gaccttcgat ctgcgctgcg ccaccggtta atacaatgcc tgccgccagg tgatgtttaa
    45241 ccccttgttg gcgaagcttt tcctgcaact gcaatatctc ttcgttgacc aggttgagca
    45301 gctcggtata gcgcggctcg atcacctctg ccagggtctg acgttgcaga ctccgcggcg
    45361 gacgaccacc tacgctcggc acttccacgc tctcatcttt tccaacgatg gaacccagcg
    45421 cacaaccgtg gcgaacttta atcgcttcgg cgtcgcttgg cggcgtgcca aaggcgtaag
    45481 cgatatcact ggtcacgaca ttgccagcat aaggaattac cttagtgtgg cgcaatgccc
    45541 caccggtata aacggcgata tccattgtac caccaccgat atcgacgacg cagacaccca
    45601 gttcacgttc atcttccgtc aataccgaat aacttgatgc cagtccggca aatatcagtt
    45661 ggtcaacttt cagcccacaa cgttcaaccg ctttgacgat gtttttcgcc atatcgttgt
    45721 gacatgtgat caggtgcact tttgcctgca tccgcacgcc cgaaagtcct accggattct
    45781 tgatcccttc ctgatagtca atcgcatact cttgcgggat cacatgcagc acacgatgtt
    45841 catcgcgcac acgcaccgat ttcgcggtat ggacgacgtt ttccacatct tcttgcgtca
    45901 cttcttcttc agaaataggc accataccaa tttcattctg gcagctgatg tgcttaccag
    45961 aaagcgccag atataccgaa gagatctgac aatctgccat caattctgcc tggtcaatgg
    46021 cgcgttgtac gcacttgacc acggattcga ggtcgttcac cccgccttta tccataccac
    46081 gtgacgggca gctgcccacg ccaatgatat tgaccatacc gtcgggcaga acttccccta
    46141 ctaaagcggc aaccttcgcg gtaccaatct ccagtcctac taccagtttt ctgtccgtcg
    46201 ccttgatcat tgttgttctg cctgtgcctg attttgttgc tgagtagatt cctctggcgg
    46261 caagggcgcc cagcctactg ccgctccaga gtcataacgc aaatcaacgt agctaatccg
    46321 tttgccatcg gtttgcgcct gctgctgtaa aaccggataa agttctacaa agcgagccaa
    46381 acgtttcatc gtatcgcccc ggccaagatt gagcttaata tcgttattca gcgtcaactg
    46441 ccaggaacgc cgcgcggtca tcgccgcttc cttcagagta aatctgtcct ttgccagcat
    46501 ctgccccatt tcgcgatagc cctgcaacac ttcattggcg ctgccttccg ggccatacag
    46561 cattggaagc acctgcttgc tggtgcgatc tggcggcacg ctgaaggtgt ttccttccgc
    46621 gtctaccatg tgttgatcat tccaccgcgc aatcggcaca tattcaacca gatgaatctt
    46681 caattcatca ggccactgct ttctgacgct cacctgctta atccacggca ggcgttgttc
    46741 tatttgcgtc tggatgatgt tgacatcctg ggtcataaag gtacccggct cacccaatgc
    46801 caggatcgac tgccggatat cgtcattacg cgtgtaatgg cgttcaccgg tcaacaccag
    46861 ctttgagagc ggcaggcgtt gcgcatcttc catccagccc aacacgaccc agccgctcac
    46921 caacactgtc gttaaaacgg tcagcaggaa aaggatcccc gccagacgcg ttccattatt
    46981 gcggcgagaa gaaacctctt cttcgctatt tcgcgtgttc agagcagcct gcgacatatt
    47041 agtccgccag ttccagaatt cgtactacca actgcgagaa gctcatacct gcctgacgtg
    47101 ccgccatcgg caccaggctg tggctggtca tacccggtga ggtattggct tccagcagat
    47161 aaaactgtcc atcgctgtcc agcataacgt caatacgtcc ccatcctttg caacctaacg
    47221 tcgtccatgc tttcagcact aatgcctgca aattggcctc ttgtgacgct tccagacctg
    47281 cggggcagaa atactgtgtc tcatcagaga gatacttcgc ctcataatca tagaaggttc
    47341 cggacggttg aatacgtatt gacggtaaaa tttcttcacc gagtatcgca accgtgaact
    47401 ccggcccact tagccatttt tcaatcaata cttcttcatc gtgctgaaat gccaatctta
    47461 atgcatcttg tagagcattt tctgctacta cttttgacat tcccacactg gaaccttcgc
    47521 ggctcggctt aacgataacc ggcaaaccca gagcagaaat ttctgctaac tgcttatcgc
    47581 tcaggccttt ttcaaactct gcgcgggtta acgctaccca cggcgcgacc ggtaaaccgg
    47641 caccttgcca cagcagtttg ctgcgtagtt tatccattga aagcgcagat gccatcactc
    47701 cgctgccggt ataaggcaag cccatcagct cgagcatgcc ctgcagcgta ccatcttcac
    47761 cgccgcgacc gtgtagcgcg ataaacactt tctgaaaacc catcgacttc agttgcgtca
    47821 cgtcgacttc tttcgggtcg acaggatacg catcaatacc gccttcacgc agcccggcta
    47881 acaccgctgc gccagaattc agagaaactt cccgctcagc ggaagtcccg cccaacagga
    47941 ccgcgatttt atcagtcatg ttgttcttcc tccggagttt gcggcttcag tttgatttca
    48001 gctaaagaac gggcaatttt tccgatatta ccagccccct gaacgagaat caggtcgtta
    48061 ccggttaata ccggtgccag catctcggct acctgcgcag ggtccggcac cagaatggga
    48121 tcaattttcc cacgtccacg aattgtgcga cacagcgaac ggctgtccgc tcccggaatt
    48181 ggcgcttcgc cagccggata cacttccagc atcaacaggg tatcaacctg cgtcagcaca
    48241 ttggcgaaat catcatacag gtcgcgcgta cgggtaaaac ggtgcggctg aaacagcatt
    48301 accaggtttt tatccggcca gcctgcgcgc gccgctttaa tggtggcgtc aacttccgtc
    48361 gggtggtggc cgtagtcatc gaccagcatt gccgtaccgc ttttaccatt cactggctcc
    48421 agcgggaatt caccgaggaa atcaaaacgg cgcccagtcc cctggaagct ctccagcgca
    48481 cgtaaaatag cctcgtcatc aattccctct tccgtagcaa ccgcaaccgc agctgcggcg
    48541 ttcagcgcgt tatgacgacc tggcgcattc agggtgacgc gcatcggctc tttgtcctgg
    48601 cgcagcagcg taaagtgccc ctgcgggcca atctgctgat aatcttctac acgcacgtcg
    48661 gcatcttcgc tgaagccgta agtcgtggtc tgacgtccca ctcgcggtaa caattcgcgg
    48721 atcaccggat catcaacaca catcaccgca cgaccgtaaa acggcaggtt gtgcagaaaa
    48781 ttaataaaag tctgttttaa attctcaaag tcgccctggt aggtatccat gtggtcggct
    48841 tcgatattgg tgacaatcgc caccatcggt tgcagatgca ggaacgatgc atcactctca
    48901 tctgcttcgg caatcaggta ccgaccatgc cccaaacgcg catgaacccc cgccgctttt
    48961 accagcccgc cgttaacgaa ggttgggtcg agccccgctt ctgcgtagat gctggaaacc
    49021 atcgcggtgg ttgtcgtttt gccgtgcgtt cctgcaatgg cgatgccatg acgaaaacgc
    49081 attaactcag ccagcatttc ggcacgacgg atcaccggaa tacgcgcttc atgagctgcg
    49141 acaatttccg ggttatcggc agaaatggcg ctggaaacaa cgaccacgct ggcatcacgt
    49201 acgttttccg ggcgatggtt gaaataaatc gtcgctccca gattcattaa ctgctgcgtg
    49261 accggatttg gcgctaaatc ggaaccactg atctgataac cttcattggc cagaacttcg
    49321 gcaataccgc ccataccggc accaccgatg ccgacaaaat gtatgtgccg aacgcgacgc
    49381 atttcgggca cgatggaacg cagttttgcc aattgttgtg tattcattct ttacgccatt
    49441 aacttcttaa attcatacga tgcaaaaggc atcgctacaa ttacgcccgg gcagcccggc
    49501 tcacttcatt tgccactcgc tcggtggcat ccggaatgga tgcagcgcgg gcgcgttctg
    49561 ccatggttaa taaggtttct cgcgaccacc cggccagggt gttggcgaca gcatccacgc
    49621 taagctgtgg ctgctcgata attttggctg cgcccgcttt ttccagcggt agcgcattcc
    49681 agtattgctg gcggtcttta tgttgaaacg gcacaaacaa cgccggtagt cctgccgcgg
    49741 cgatttcact caccgttaaa gcaccggagc ggcaaacgac gacatccgcc cacgcatacg
    49801 ccgccgccat atcatcaata aattccgtca ctttatgctg cggttgcccc gcttcggcat
    49861 acgcctgttc aacggattgt tgcgaacctt taccgctctg atgccagata gtgactgaat
    49921 cacccagttt cgcagcaacc tgcggcattg tctggttaag aatgcgtgcg ccctgggaac
    49981 cacccactac cagcacacga accggacctt cacgtccagc caaacgttgc tgcggcaacg
    50041 gcagcgccag cacatcggta cgcaccgggt tacccaccac ttccgcatta gggaaagcgc
    50101 ctggaaacgc ctgcatcact ttggtggcaa tcttcgccag ccatttattg gttaagcccg
    50161 caataccgtt ttgttcatga agtacaaccg gaatgcctaa cgaccacgcg gccagaccac
    50221 ctggacctga tacatagccg cccataccga gtaccacgtc aggtttgtac gctttcataa
    50281 tcgcccgcgc ctgacgccag gcgttgaaga tacgcagcgg cgcagctatc agtgctttta
    50341 taccttttcc acgcagacca gagatacgaa tgaaatcaat ttcgatgcca tgttttggca
    50401 ctaagtccgc ttccatacgg tcggcagtcc ccagccagcg aacttgccaa ccctgagcca
    50461 ttagatggtg cgcaaccgcc agtcccggga atacatgtcc accggttccg cctgccatca
    50521 ccattaatcg ctttccttga ccactcatcg tgaacctcgt acaaacgcct gcgctttctc
    50581 cagacgcgtt tcataatcaa tacgcaacag catcatgatg gctgtcgaca taatcagtaa
    50641 gctcgaacca ccgtaactga tcagcggcaa tgtcagacct ttggtcggta acatccccgc
    50701 cgccgcgcct acgttaacca gcgcctggaa gctaaaccag atgccaatag aacaggcgag
    50761 aaaaccggaa aaacggtggt caatttctaa cgctttgcgg ccaatcgaca tcgcgcgaaa
    50821 agcgacgaag aataccatta aaagtgccag caccacaccg acatacccca gttcttcgcc
    50881 gataatggcg aaaataaagt cagtgtgcgc ttccggcaga tactccagtt tttgtaccga
    50941 gttacctaaa ccttgtcccc aaagttcgcc gcgaccaaac gccatcagcg attgcgttaa
    51001 ctgatagccg ctgccaaagg gatcttccca cgggttccag aatgcggtaa cacggcggat
    51061 acggtacggt tcggcgagta tcagcaacac aaccgctgaa atgcccatac cgataatggc
    51121 aatgaactgc cacaatttcg ctcccgccag gaacaacatc gccagcgtag tcacaaacaa
    51181 caccaccacc gtaccaaggt ctggctgtgc cagcagtaac actgccaaca ccagaatcac
    51241 gcccatcggt ttcaggaagc cgcgcaggtt attacgtact tcgtcgcctt tacgcaccag
    51301 atagttggcg atataacaaa acagcgacag ttttgtcagc tccgcaggct ggatacgcag
    51361 caaaccgaga tcgatccaac gcgatgcccc tttaaccgag ctacccacta ccagcacgat
    51421 catcagcagg atgatagatc cgagcagcat cgtggcactg tagcgttgcc agaactccat
    51481 cggcagacgc agcgtaatga tcgccagaat gaatgccagg atcagataga cgccatcacg
    51541 cttcgcgaag aagaacggat cgttggttaa gcgttgccct atgggcattg acgccgaggt
    51601 caccatgata aagccaatcg ccgcaaggcc gaaggtcagc cacagtaagg tgcgatcgta
    51661 catgatcagg ctgtcggtat ctttttcccg cgagcccatc acccagcctt ttagcgccgt
    51721 ggagatccag accaggatac tgaatcctgg caggcgcggc attttcaggc gagggagaga
    51781 taaacgcatc aacctaactc cttcgccaga cgggcaaact cattgcctcg ttgttcaaag
    51841 ttcttgaact gatcaaggct ggcacaggcc ggggagagca gaaccatatc gcccggctga
    51901 acacgcggag ccagcaagcg catcgcctgt tccatagttt cggtttgttc tgccacttcc
    51961 gggcgtagcg ccgccagctg cgcaccgtca cgaccaaaac agtacaaacg cacgttgtcg
    52021 ccattcagat agcgcgccag tggactgaag tccgccgatt taccatcgcc gcccagcaac
    52081 aaatgcagcg tgccgtctac ctgcaagcca ttcaacgccg cttccgtact gccgacgtta
    52141 gtggctttcg aatcgttaat ccagcgtacg ccattgtgct ccagcaccac ttcaaagcga
    52201 tgcggcagac cggtgaaggt ggttaacgct ttcaggctgc tggcgcgcgg caaaccagct
    52261 gcgtctgcca gtgccagtgc tgccagcgca ttggtgtagt tatgctgccc ggaaagtttc
    52321 atctctttca cgttcagcac tttctcgcct ttcactcgca gccaggtttc tccctgctga
    52381 tgattcaggt gatagtcacc catgttgacg ccaaagctga cgcagcgttc atcagcaccg
    52441 cgaatcggca ttgttaaggc atcatcagca ttgacgacac aaactttcgc attttcgtaa
    52501 atacgcaatt ttgccgcacg gtattgctgc aaaccaaacg gatagcggtc catatgatct
    52561 tcagtcacgt tcagaatggt cgccgccacc gcctgtaagc tggaagtggt ttccagctgg
    52621 aagctcgaca gttccagcac gtacagttcg cactcagcat caagcagcat caacgctggc
    52681 aggccaatat tgccacccac accaacgtta acccccgccg ctttcgccat ttcacccact
    52741 agcgtggtga ccgtgctttt gccgttagaa ccggtaatcg ccacaatcgg tgcttgtgct
    52801 tcgcggcaga acagctcgat atcgccaacg atttcgattc cggcatcagc ggcagcgctt
    52861 aaggatggat gcgccagtgc aataccggga ctggcgacaa tcagatctgc cgccatcagc
    52921 cattcatcat tcagactgcc cgtgtggcgt tctacggctt cgggtaattt atccaggcca
    52981 ggcggtgtca tacgcgtatc cataacgcgc ggcgtcagac cgcgagcgag gaaaaagtcc
    53041 acgcaggaaa gcccagtgag gcccaggccg ataatgacga catttttacc ctgataatca
    53101 gccatgatta acgtaccttc agcgttgcca gaccaatcag aaccagcatc agcgaaataa
    53161 tccagaaacg cacaatgacg cgcggttccg gccagccttt cagttcatag tggtgatgaa
    53221 tcggtgccat gcggaaaata cgttgtccgc gcagtttaaa ggagccgacc tgcaggatga
    53281 cagaaagcgt ttctaccacg aacacgcccc ccataatcac cagcaggaat tcctgacgta
    53341 gcagtacggc gataatgcct aacgcaccac ctaacgccag cgaacctaca tcgcccataa
    53401 agacctgcgc cggataggtg ttaaaccaca ggaagcccag tcctgccccg actatcgcgg
    53461 tacagacaat aaccagttcc cctgcgtgtc gcagatacgg tatatgcaag tagctggcaa
    53521 agttcatatt gccggtcgcc cacgccacca gcgcaaaacc accggcgaca aatacggtcg
    53581 gcataattgc caggccgtcg agaccatcgg tcaggtttac cgcgttgcca gtacccacaa
    53641 tgacgaagta agccagcaga atgtagaaca gccccagctg cggcatcaca tctttaaaga
    53701 atgggaccac cagctgcgtt gcgggcgtgt ctttgccggc aaggtacagg gcgaaggcga
    53761 cacccagcgc aatgaccgac atccagaaat acttccaacg agcgatcaac cctttggtgt
    53821 ctttacgcac cactttgcga taatcatcaa caaagccaat aacaccgtaa cctaccagca
    53881 ccaccaacac gcaccagacg tacggattgg acgggtaagc ccacagcagt acggagatca
    53941 caatcgccgt caggatcata atcccgccca tggtcggcgt accgcgcttg ctgaagtgtg
    54001 attcaggacc gtcgttacgc accacctgac caaaggaaag tttttgcaaa tgagcaatca
    54061 tacgcgggcc catccacaat gagatgaaca gcgcggtcag caggctgacg atggcgcgaa
    54121 acgtcagata ggaaaagacg ttaaagccgg aataatattt gaccaaatgt tcggccagcc
    54181 aaactaacat gtcccattct cctgtaaagc gcgtactacc tcttccatgg cggcactacg
    54241 tgaaccctta actaaaatcg taattacctg ttgctcagca atcagtgatt taagacgcgt
    54301 aattaacgca gttttatcag caaaatgttc gccaacgccg ctggcggtgc tgatagcatg
    54361 gctttgttta cccacgctta acacgcggtc aataccagca gcttttgccg cctcgcccac
    54421 ctgtacatgg caggcttcgc tttcagcgcc cagttccgcc atatcgccca ccaccagcac
    54481 gcggtagccc ggcatttcag ccagtacctg aacagcggcg gtcattgaac cgacattggc
    54541 gttgtaggag tcgtcgagca gcaactggtt ttctgccagt tggatgggga acagacggcc
    54601 tggaacagct ttcagatttg ccagccccgc tttgatagca tcaagcgttg cgcccacgga
    54661 catggagagc gcagcggctg ccagcgcatt cgcaatattg tgacgccccg gcaacggcag
    54721 cagaacatcg acgctaccgg ttggggtttg tagggtaaat tccgtaccgt gcgaggtcac
    54781 atggatattg gtggcggtga aatcgctgtt ggcggcattg ggtgagaaac gccacacttt
    54841 gcgtgagcca attacgctct gccagttcag ccagtcgttg ttgtcggcgt tcataatggc
    54901 gataccgttt tccggcaggc cgctaaagat ttcacctttc gctttcgcga cacccgcaag
    54961 cgagccaaaa ccttccagat gcgccgctgc caggttgttg accagcgcag cttccgggcg
    55021 agtcagactc acagtccagg ctatttcgcc ctgatggttc gcgccaagtt caataactgc
    55081 gtaatcgtat tccggcgtta agcgcaacag cgtcatcggt acaccgatgt cgttgttgag
    55141 attgcctgcc gtataaagcg tgttgccgca ctggcttaaa atcgccgccg tcatctcttt
    55201 aacggaggtt ttgccggagg accccgtcag agcaaccacg cgcgccggaa cttgctggcg
    55261 aacccatgca gccagttcac caaacgccag acgcgtatcc ttgacgatta actgcggcag
    55321 gtcgatgtcc agcggacggc taaccagtag tgcgcctgcg ccgccagctt tcgcctggtc
    55381 ggcaaaatcg tgggcatcaa aacgttcgcc tttcagggca acaaacaggc agcccggcgt
    55441 cagttttcgg gtatcagtgg ttacagcatc aagggtgata tctgcacctt gcagttcacc
    55501 gttgagaatg tcggtaagtt ggctaagggt tacgctaatc atgcaatcac ccccagcaga
    55561 cgcgccaccg tgacgcgatc ggagtagtcc agacgctgat tgccaacaat ctggtaatct
    55621 tcatggcctt tgcccgcgac cagtaccaca tcattctctt tagcctgcat aacggcgcaa
    55681 gtcaccgctt cagcacggcc ttccatcact ttggcatgtc cggcatctaa cattcccgcc
    55741 agaatatcgt tgatgatggc acgcggttct tcggtacgcg ggttatcgtc cgtcaccacc
    55801 gccacgtcag caaactcttc ggcaattgcg cccatcagtg gacgcttacc tttatcgcga
    55861 tcgccaccac agccaaagac gcaccacagc ttgcccgcac agtgcaggcg cgccgcctgt
    55921 aaggcttttt ccagtgcatc cggcgtatgc gcgtaatcca ccaccaccgt cggtttgcct
    55981 ggcgcagtga acacttccat acgtccgcaa accggttgca gacgcgcggc ggttttcagc
    56041 agatcagcca gtggatagcc gagtgccagc agtgtcgcca gcgcgagcag caggttgctg
    56101 acgttaaaag cgcccatcag atggctttca atttcgccat cgccccaact tgagctaaag
    56161 cgaatcgtcg caccgctgtc gtgatagttc acgtcgatcg cttttaacca gcgtccgtga
    56221 cagttcggat tgatatgatc ttccattgat accgcaaccg cgtccggcag ttttgccagc
    56281 cagcggcggc ccacttcatc gtcggcgtta ataatcgcct gaccgcaatg atgttcagaa
    56341 taaagcagcc atttcgcagc ttcgtagtgt tccatatcac catgataatc aaggtgatcg
    56401 cggcttaagt tggtaaagac cgacgccgca aatttcaatg ccgccacacg gtgctgtacc
    56461 agcccgtggg aggaaacttc cattgcgcaa aacgtcgcgc cctgctccac cagccccgcc
    56521 agctcatgct gaacatcgac tgcggaaccg gtagtatttt ctgtcgggat cactttccca
    56581 agcagaccgt taccaacggt gcccattacc gcgctggttt cgccaagcag ttggctccac
    56641 tgcgccagca gctgggtagt cgtggttttg ccgttggtgc ccgttacgcc tacgagacgt
    56701 aaattgtcag agggttcatg gtaaaagcgg cccgccagtg cagataaacg ctcgttgagc
    56761 tggctgagat agatgaccgg tacgccgtgc atttcacgga tttcaccatc ggtcgcctca
    56821 tctttcgcct ctgcaataat ggcagccaca ccttgcgcta tcgcctgcgg gatatatcga
    56881 cgcccgtccg cctgatgacc tactacagct acaaagagat cgcccgccgc agccacacgg
    56941 ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt ctggcaccca cggagcaaga
    57001 aggtcgcgca aattacgatc tgccacctgt cccctcgcct tgattaatca caaattcatt
    57061 tttatcgccc gttgtcagcg catccggctc gatgttcatg gtacgcaata cgccgcccat
    57121 gatggcacca aagaccggcg cggaaacggc gccgccgtag tatttacccg cctgcggatc
    57181 gttgataaca acaaccagcg cgaagcgcgg ctgactcgca ggcgcaacgc ctgcggtata
    57241 agcaatatat ttattgatgt agcgaccgtc cggcccgacc tttttcgcgg taccggtttt
    57301 aatggcgata cgatagcctt taatcgccgc tttcacgccg ccgccgcctg gtagcgccac
    57361 gctttccatc atatgcacca cggtgcgaac aatggattcc gggaagacac gttcaccggg
    57421 aaccgggggg tcaactttgg taatcgacag tgggcgataa atgccgtagc tgccgatagt
    57481 tgcgtagact cgcgctaact gtaacggtgt taccattagc ccgtagccga aagagaaggt
    57541 ggccctctct atgtcagacc accgttgttt ttgaggatat aagccactgc gttctccgac
    57601 caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag taagtatcta ctaacgctga
    57661 ggacggcatc gctaacgcca gcttggaaac accgacgtta ctcgacttct gtaatacccc
    57721 ggtcagggtt aattcgctgt agcgtgccac gtctttgatt tcgtggccgt taattcgata
    57781 aggaatggta ttgagtaccg agttttcccg caccacgcca cgttgcaacg cggtcattac
    57841 caccatcggt ttaaccgttg agcccggttc aaacacgtcg gtgatggtac ggttacgcat
    57901 cgcctctttc ggcgtgccgc tcagattgtt agggttgtat gacgggctgt tagccatcgc
    57961 cagcacttca ccggtgttga catccaccag cacggcgcta ccggattcag ccttgttaaa
    58021 ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc aagcgttcat caatactcaa
    58081 cgccaggttg tgcgccgcct ggctgtcagt agaagaaata tcttcaatta cgcgaccata
    58141 gcggtcttta cgcacaatgc gctcacccgg ctgcccggta agccatttat cgaaactctt
    58201 ctcaacgccc tcaatccctt gactatcgac gttagtaaag ccgatgaggt gagcagtcac
    58261 ttcgccggac ggatagtaac ggcgagactc ttcacgcaga tgaatccccg gcagtttcag
    58321 ttttttgatg tagtccgcca tgtcagggtt cacctgacgc gccagataaa taaagcgccc
    58381 tttcgggttg gcgttaatgc gggctgaaag ctgatccagc ggaatattga gcgcgttagc
    58441 cagcgccttc cagcggtcac cgacgctgat accgccagcg tcatgcactt ctttcgggtc
    58501 agcccaaatc gcttttaccg gcacgctcac cgctaacggg cgaccagaac ggtcagtaat
    58561 catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa cgcatgtcgc cctctttcac
    58621 cagcatatcc ggggagataa cttgtaacca cgctacgcgt ccgagcagaa aagccagcgc
    58681 caggagaata cagccgcata acaacgcaaa acgccaactg ataaagttgg catgttcttc
    58741 ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg cgtcgcgttt atccttattt
    58801 ttgcactacg atattttctt gtgacggatc aacatgctgc atttgcagct tttccgtggc
    58861 gatcctttcc acccggctat ggtcgccgag cgcattctct tcaaggatca ggttgcgcca
    58921 ttcaatgtct aaagcatctc gctccagcac cagttgttcg cgctgagcgg tcagtaaacg
    58981 ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa ataatgcaaa tgaacaggca
    59041 gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata acaccaggca atgcatggcg
    59101 ctcgtggctt cccatcgatc ctttaacttt gcttagagct tctgtcactc tgctgatcat
    59161 gcattcgtcc tctctgcaat acgcagaact gaactacggg cacgagggtt ctcagccacc
    59221 tcttcttcgc ccggcattaa cttgcctagt gctcgcagct gacggccacc cagttttttg
    59281 agctgctctt cagtcatcgg taaccctgcc ggaacttgcg gaccgcggct gttttcacgc
    59341 ataaaacgtt tcacaatacg gtcttccagc gagtggaagc tgatgatcga aagccgccca
    59401 cccggggcca gcacgttgag cgagcttttt agcgcctgct ctatctcctc cagttcactg
    59461 tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg gatgtttaaa cttatctttc
    59521 accggcgttg cagcagccac gacttccgcc agttctttgg tgcgggtcat cggctgttcg
    59581 cggttacgct cgacaatggc gcgggcaatg cgtttggcaa aacgctcttc accataggtt
    59641 ttcaataccc aggcgatatc ggcttcttct gcggtttgta gccattcagc ggctgactgc
    59701 ccacgggttg ggtccatacg catgtccagc ggaccatcgc gcataaagga aaagccgcgc
    59761 tcagcatcat caagttgcgg tgaagagacg ccaagatcga ggagaatgcc gtcgatcttg
    59821 ccgataagat cgcgctcggc aacgtattcg cccagcgcgg agaaaggtcc gtggatgatg
    59881 gagaagcgcg gatcatcaat agtcttcgca acggcgatag cctgcgggtc gcgatcgatc
    59941 gccagcaaac gcccctcttc gccaagctgc gagaggatca gacgtgagtg accaccgcga
    60001 ccaaaagtcc catcaatgta gatgccatca ggacggatat tgaggccatt aacggcttca
    60061 tccagcagca ccgtagtatg tttatagttt tccatcattt tatagagaca agtcctgcag
    60121 tcgctccgat aagtctccgg tagccaactg ctctgcgtcg atatcttcct tgacctgttg
    60181 atgccaggtt gtttcatccc acagctcaaa cttgttgaac tgtccaacca gcatcacttc
    60241 ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt aacaatcgac ctgcgccatc
    60301 catctgacat tcgctggcat gacctaacag taggcgctgc acacggcgct caaccgggtt
    60361 catgctcgac agacgcgata atttttgctc gataatttcc cattcaggca gggggtaaag
    60421 cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt tgaccggcag cgttctcaag
    60481 cagctgttcc cggtaacggg taggcactga taagcgccct ttgctgtcga gattgactaa
    60541 cgttgctccc cggaacatgc cagcctcacc ccttattccc actttgcccc acaatttccc
    60601 actgaaagga gtttacggag cggaggaaaa gcttgtcaag ccagtcacaa ggcataccga
    60661 ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt tattttacgt ccaacgtgcg
    60721 cagcaaatta acgttatgaa tagctgtaag aaaaaatccc tcatcgtcat caccgtttta
    60781 aaatgactca ctattttcca cagcaaatat aaagtgtcag tttgcgacgc gagcggcatt
    60841 ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc tgtgtggcgc ttgatgccag
    60901 cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg gcaattcatc tctgcatgta
    60961 acaaaataat gcaaaaacgc gctgtcgata tcacttaatt attgtgaatt tcagctggtg
    61021 atttaattaa cgggcgggtt taaaagaact tatcaataat agtcaggaat agtcttattt
    61081 actttaagga tattgatgtc cattcccgta ctctacgcgc cagagggaaa ttcacctggc
    61141 gcgtattttt gttcgcggct tagctacggc tgagcacacc gcgacgatag agattacgtt
    61201 taatgcgcgt taaaccaggt tttggcttac gcggttcgtc caggcttgcc aggacaatct
    61261 ccagcacacg ctctgcgaca tcgcggtgac gttgagccac tgccagcacc gggcactgta
    61321 agaagtcgag cagttcgtta tcgccaaagg tggcaattgc caggtcagaa ggcagtttac
    61381 catcgcgacg tagcgttaca tccatcaccc cttgcaacaa cgcaaacgac gttgtgaaca
    61441 gcgcctgcgg catcggatgc gtttccagcc atttttcgaa taactgggca gccgcctccc
    61501 gctcatagct gttggcatac aggaaatgca cttcgcgcgg atcatctttc caggcagtac
    61561 ggaaaccttg ttcacgcagg aagctgacag aaagctccgg cagcgcgcca agataaagca
    61621 ccgtctcggc gggaaactta cgtaactctt ccgccagcat ttcggcatca tcctgatcgg
    61681 caccaaccac gctggtgaag tgttcacgat cgagggcgcg gtccagcgcg acaatcggga
    61741 acgggtcgtt agcccagcgt tgataaaaag gatgctcagg aggcaacgac gtcgaaacaa
    61801 taatggcatc gacctgacgc tgtaaaaggt gctcaataca gcgcatttcg ttgtctggct
    61861 gatcttctga gcaggcaatt agcagttgat aaccccgttg ccgcgcctgg cgttcaagat
    61921 agttagcgat gcgggtatag ctggtgttct ccagatcggg gatcacaaga ccaatagaac
    61981 gtgtgcgtcc agcacgaagc ccagctgcca cggcgttcgg gtggtaattg tgctcacgca
    62041 ccacagccat gactttttca acggttttgt cgctcacacg gtattgcttc gctttgccgt
    62101 taataacata gcttgcagtg gtccgcgaca ctcccgccag ccgagcgatt tcatccagtt
    62161 tcacaattgc cccttgcgta aaatgtaaaa accatagcca ttgtactggt atgggttaaa
    62221 ttcattaaca tctaagcgca gaaaattaac tgcggcaact gcttttattc caggttccca
    62281 ctgatttcgc aaaaaaagcc caacgtgaca tgttgggctg caaattgcgc actgagatca
    62341 ttctcaacgc attattttat cgccgcgcga aagtccgacc acaccagagc gagcaacttc
    62401 cacaattttc gccacatcgc gaatcgatgc taaaaatgca tcaagcttat cgctggtgcc
    62461 tgctaattga acggtataaa gcgagggggt gacatcgata atttgcccac ggaatatttc
    62521 cgtattacgt ttcacttcgt cacgcccgta accgctggcc tgaattttca ccagcatgat
    62581 ttcccgctca acatgcgcgc cctgccccaa ctcactcacg cgcaagacat cgaccagctt
    62641 gtgtaattgc ttttcgatct gctcaagcac tttttcatcg cccacggtct ggatggtcat
    62701 acgcgataat gtcggatcgt cggttggcgc aacggtcagg ctttcaatgt tgtagccacg
    62761 ctgggaaaaa aggccaatca cgcgggataa cgcgcctgat tcattttcga gtaagactga
    62821 taatatccgg cgcataatca ggttctctcc gttttgctta accacatttc atccattccg
    62881 cccccgcgaa tctgcatcgg gtagacgtgc tcactgccat cgacggtaac atcaacaaac
    62941 accaggcgat tattgcgcac ctgttccagc gcctcgctaa gtttgctttc cagctcatgc
    63001 ggatgagaga tctggatccc gacgtgacca taggcttccg ccagacggac gaaatcgggt
    63061 agtgattgca tataagattg tgaatgacgg ccggaataga tcatgtcctg ccactgtttc
    63121 accatcccca gatagcggtt attgagattc accaccagta ccggcaactc gtattgcaac
    63181 gcggtagaca gttcctggat gttcatctga atactgccgt cgccagtgac gcaaaccacg
    63241 gtttcttctg gcaacgccat tttgacgccc agtgccgcag gtaaaccaaa acccatcgtg
    63301 ccgaggccac cggaattgat ccagcgacgc ggtttgtcga atggataata aagtgcagca
    63361 aacatctggt gctgcccgac atcggacgtc acgtaagcgt ctcccttcgt caaccgccaa
    63421 agagtctcga tcaccgcctg cggtttaatc ttttcactgt gagtgtcata tttcaggcac
    63481 tgacgagcgc gccactgttc aatctgctgc caccagtcgc ggatctcatc cagcggttga
    63541 tgggcggatt cttgcgacag gagttcaagc atttgttcga ggacctggcg agcatccccc
    63601 acaatcggga tatcagcagt cacggtttta gaaatggaag taggatcaat atcgatatgc
    63661 agaacagtgg catttgggca gtactttgcc agattattcg tcgttcggtc atcaaatctt
    63721 accccgacgg cgaaaatcac atccgcgtta tgcatcgtca tattggcttc gtaggtgccg
    63781 tgcattccca gcatgcccag cgcctgacga tgcgttgccg gaaatgcccc caggcccatc
    63841 aatgaggaaa caacgggcag attcaacgcc tccaccgttt ctttcaactg ctgatggcag
    63901 cccgccgtga ttgccccacc gcctacgtag acaaccggtt tttttgccgc caccagcgtt
    63961 tgcagagcac gcttaatttg ccctttatgt ccggtagtag tgggattgta agaacgcata
    64021 ctaaccgact ccggccagac atagggtaat ttgttcgccg gattaagaat atctttcggt
    64081 aaatcaacga ctactggtcc aggacgacca cttgccgcca gccagaaagc ctttttcagc
    64141 acctgcggaa tgtcttccgt ttgcttaacc agaaaactgt gtttaaccac cggtcgcgaa
    64201 atccccacca tgtcgcactc ctgaaaggca tcgtaaccta tcaacgaggt cgctacctgc
    64261 ccggaaagga caactaatgg aatggaatcc atataagcgg tggcgatgcc ggtaatcgca
    64321 ttggtcgccc ctggacccga cgttaccagc acgacgccga cttccccggt cgcacgcgcc
    64381 aggccatcgg ccatatgcac cgccgcctgc tcatgacgaa ctaatacatg atcaatacca
    64441 cccacggtat gcaatgcatc gtaaatatca aggactgcgc ctccgggata accgaatact
    64501 tgtttaacgc cctgatcgat aagcgatcgg acgaccatct cggctccaga caacatctcc
    64561 atggcctgcc tcactgtttg acggaaaaaa tgtgtaaaac acattaaccg tccgatcttg
    64621 cttaagcaat tggcagccag agacataaac gcagggtaat aagaataaac aggaggaaag
    64681 gtgaaaagaa acgataaaat cctccattac gaaggattaa attgacaata aatcggctga
    64741 atcccacaac ttacagcaat ttctctattt ttttaattaa tcctaaaata gagagcacac
    64801 acattcagaa taaaattcta cattcaataa aaaatgcaga ataaaccaga cattcatgtc
    64861 tggcctattc tgcattcagt tagcgtttgc aaattgagac taattgttct tccatccact
    64921 gatggccttt atcgcgcccg gcagcttcat gccaggagag ataacaggtt ctgctgtttt
    64981 gttttaacgg cagcggtaat acctgtaatt ctaaggattc agcgaactct tcagccagcc
    65041 aacgcggcgc aatagcgacc aaatgcgttt gcgacaccac gctaagtacg ctcatcattg
    65101 ccatgccctg atacgcgata ctggcttgct tatctaccgt gtcataccaa ggttgactaa
    65161 atgacgcgaa acgatcgagc gaaaccgccg catgttgttc gttataaaca tcatgtttca
    65221 gtaacgggcc cttaattgtt ggatgatttt tgctggctac caacaccatt tcatctttaa
    65281 ataatggcac gctggtaaat tcaggacgat ggaagtcttc ataactaatc acaaactccg
    65341 tttcctgata acgcagctga tgttcagtgt tctgatttaa tgaagacttg aacataacat
    65401 gtatatttgg cgcaatctgc tcaatgtgat tataaatctg cgaggtcaga atgctgtcta
    65461 acgggctgca aacacaaaga tgaaatacac gttcactgct cgcgggttca aaacctgaac
    65521 caggcaattc attttgtact agttgcaatg cctgacgaac tgagccaaaa agttgaaatg
    65581 cgcgagcagt cggttgaata ccacggccat aacgaacaaa aagctcgtca ttaaacatca
    65641 ccttcaggcg tgcaacagcg ttactgaccg caggttgcga catacccaga acatgagcgg
    65701 cacgggtaat gttttgctcc tgcatcacgg catcgaaaac ggttaataag ttgagatcga
    65761 ccatgcgtag ctgtggcttg cttaactccg ccgtctctga atgatctgtt tgtacctctg
    65821 gcatacttaa ctccactgtc acacttaact ccctttccct tattggaatg cagagaaata
    65881 atcctgaaga atatgattta tcatgcatat aaaataagaa aaagcaaaat gagtaaaatt
    65941 cggaaaacat aaagatacgg gttctgataa aaacagaatc cattgccgtg
//
LOCUS       DAPGWD010000007        65660 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPGWD010000007 DAPGWD010000000
VERSION     DAPGWD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.007"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            87..650
                     /gene="srlA"
                     /locus_tag="RHX88_000402"
                     /gene_synonym="gutA"
     CDS             87..650
                     /gene="srlA"
                     /locus_tag="RHX88_000402"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDV2229136.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            647..1606
                     /gene="srlE"
                     /locus_tag="RHX88_000403"
                     /gene_synonym="gutE"
     CDS             647..1606
                     /gene="srlE"
                     /locus_tag="RHX88_000403"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDV2229137.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1617..1988
                     /gene="srlB"
                     /locus_tag="RHX88_000404"
                     /gene_synonym="gutB"
     CDS             1617..1988
                     /gene="srlB"
                     /locus_tag="RHX88_000404"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDV2229138.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGELHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1992..2771
                     /gene="srlD"
                     /locus_tag="RHX88_000405"
     CDS             1992..2771
                     /gene="srlD"
                     /locus_tag="RHX88_000405"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDV2229139.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2876..3235
                     /gene="gutM"
                     /locus_tag="RHX88_000406"
     CDS             2876..3235
                     /gene="gutM"
                     /locus_tag="RHX88_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDV2229140.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3302..4075
                     /gene="srlR"
                     /locus_tag="RHX88_000407"
                     /gene_synonym="gutR"
     CDS             3302..4075
                     /gene="srlR"
                     /locus_tag="RHX88_000407"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDV2229141.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4068..5033
                     /gene="gutQ"
                     /locus_tag="RHX88_000408"
     CDS             4068..5033
                     /gene="gutQ"
                     /locus_tag="RHX88_000408"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDV2229142.1"
                     /translation="MSEALQNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5030..6544)
                     /gene="norR"
                     /locus_tag="RHX88_000409"
     CDS             complement(5030..6544)
                     /gene="norR"
                     /locus_tag="RHX88_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDV2229143.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            6731..8170
                     /gene="norV"
                     /locus_tag="RHX88_000410"
     CDS             6731..8170
                     /gene="norV"
                     /locus_tag="RHX88_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDV2229144.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            8167..9300
                     /gene="norW"
                     /locus_tag="RHX88_000411"
     CDS             8167..9300
                     /gene="norW"
                     /locus_tag="RHX88_000411"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDV2229145.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(9526..11778)
                     /gene="hypF"
                     /locus_tag="RHX88_000412"
     CDS             complement(9526..11778)
                     /gene="hypF"
                     /locus_tag="RHX88_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDV2229146.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11931..12458)
                     /gene="hydN"
                     /locus_tag="RHX88_000413"
     CDS             complement(11931..12458)
                     /gene="hydN"
                     /locus_tag="RHX88_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDV2229147.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12607..13617)
                     /gene="ascG"
                     /locus_tag="RHX88_000414"
     CDS             complement(12607..13617)
                     /gene="ascG"
                     /locus_tag="RHX88_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDV2229148.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            13877..15334
                     /gene="ascF"
                     /locus_tag="RHX88_000415"
     CDS             13877..15334
                     /gene="ascF"
                     /locus_tag="RHX88_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDV2229149.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPVQPVGKPKLTLRRI
                     GAGILDALISTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            15343..16767
                     /gene="ascB"
                     /locus_tag="RHX88_000416"
     CDS             15343..16767
                     /gene="ascB"
                     /locus_tag="RHX88_000416"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDV2229150.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16881..17300)
                     /locus_tag="RHX88_000417"
     CDS             complement(16881..17300)
                     /locus_tag="RHX88_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2229151.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(17371..18126)
                     /locus_tag="RHX88_000418"
     CDS             complement(17371..18126)
                     /locus_tag="RHX88_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229152.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGVLQNMAGNAQLYRAD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDGFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            complement(18169..18639)
                     /gene="hycI"
                     /locus_tag="RHX88_000419"
     CDS             complement(18169..18639)
                     /gene="hycI"
                     /locus_tag="RHX88_000419"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDV2229153.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(18632..19042)
                     /gene="hycH"
                     /locus_tag="RHX88_000420"
     CDS             complement(18632..19042)
                     /gene="hycH"
                     /locus_tag="RHX88_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDV2229154.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(19039..19806)
                     /gene="hycG"
                     /locus_tag="RHX88_000421"
     CDS             complement(19039..19806)
                     /gene="hycG"
                     /locus_tag="RHX88_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDV2229155.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(19806..20348)
                     /gene="hycF"
                     /locus_tag="RHX88_000422"
     CDS             complement(19806..20348)
                     /gene="hycF"
                     /locus_tag="RHX88_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDV2229156.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(20358..22067)
                     /gene="hycE"
                     /locus_tag="RHX88_000423"
     CDS             complement(20358..22067)
                     /gene="hycE"
                     /locus_tag="RHX88_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDV2229157.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22085..23008)
                     /gene="hycD"
                     /locus_tag="RHX88_000424"
     CDS             complement(22085..23008)
                     /gene="hycD"
                     /locus_tag="RHX88_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDV2229158.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23011..24837)
                     /gene="hycC"
                     /locus_tag="RHX88_000425"
     CDS             complement(23011..24837)
                     /gene="hycC"
                     /locus_tag="RHX88_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDV2229159.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCVVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(24834..25445)
                     /gene="hycB"
                     /locus_tag="RHX88_000426"
     CDS             complement(24834..25445)
                     /gene="hycB"
                     /locus_tag="RHX88_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDV2229160.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(25570..26031)
                     /gene="hycA"
                     /locus_tag="RHX88_000427"
     CDS             complement(25570..26031)
                     /gene="hycA"
                     /locus_tag="RHX88_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDV2229161.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26243..26593
                     /gene="hypA"
                     /locus_tag="RHX88_000428"
     CDS             26243..26593
                     /gene="hypA"
                     /locus_tag="RHX88_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV2229162.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            26597..27469
                     /gene="hypB"
                     /locus_tag="RHX88_000429"
     CDS             26597..27469
                     /gene="hypB"
                     /locus_tag="RHX88_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDV2229163.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            27460..27732
                     /gene="hypC"
                     /locus_tag="RHX88_000430"
     CDS             27460..27732
                     /gene="hypC"
                     /locus_tag="RHX88_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDV2229164.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            27732..28853
                     /gene="hypD"
                     /locus_tag="RHX88_000431"
     CDS             27732..28853
                     /gene="hypD"
                     /locus_tag="RHX88_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDV2229165.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            28850..29860
                     /gene="hypE"
                     /locus_tag="RHX88_000432"
     CDS             28850..29860
                     /gene="hypE"
                     /locus_tag="RHX88_000432"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDV2229166.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            29934..32012
                     /gene="flhA"
                     /locus_tag="RHX88_000433"
     CDS             29934..32012
                     /gene="flhA"
                     /locus_tag="RHX88_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDV2229167.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32049..32402)
                     /locus_tag="RHX88_000434"
     CDS             complement(32049..32402)
                     /locus_tag="RHX88_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDV2229168.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(32479..32613)
                     /locus_tag="RHX88_000435"
     CDS             complement(32479..32613)
                     /locus_tag="RHX88_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229169.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            32689..35250
                     /gene="mutS"
                     /locus_tag="RHX88_000436"
     CDS             32689..35250
                     /gene="mutS"
                     /locus_tag="RHX88_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDV2229170.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            35356..36012
                     /gene="pphB"
                     /locus_tag="RHX88_000437"
     CDS             35356..36012
                     /gene="pphB"
                     /locus_tag="RHX88_000437"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV2229171.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(36063..36830)
                     /locus_tag="RHX88_000438"
     CDS             complement(36063..36830)
                     /locus_tag="RHX88_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2229172.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            37026..37934
                     /locus_tag="RHX88_000439"
     CDS             37026..37934
                     /locus_tag="RHX88_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV2229173.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            37931..39193
                     /locus_tag="RHX88_000440"
     CDS             37931..39193
                     /locus_tag="RHX88_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDV2229174.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDLARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            39190..39828
                     /locus_tag="RHX88_000441"
     CDS             39190..39828
                     /locus_tag="RHX88_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV2229175.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            39833..40609
                     /locus_tag="RHX88_000442"
     CDS             39833..40609
                     /locus_tag="RHX88_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDV2229176.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            40698..42062
                     /locus_tag="RHX88_000443"
     CDS             40698..42062
                     /locus_tag="RHX88_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDV2229177.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(42101..42337)
                     /locus_tag="RHX88_000444"
     CDS             complement(42101..42337)
                     /locus_tag="RHX88_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229178.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(42348..43775)
                     /locus_tag="RHX88_000445"
     CDS             complement(42348..43775)
                     /locus_tag="RHX88_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDV2229179.1"
                     /translation="MAFDDLRSFLQELDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(43775..44368)
                     /locus_tag="RHX88_000446"
     CDS             complement(43775..44368)
                     /locus_tag="RHX88_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDV2229180.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            44515..44922
                     /locus_tag="RHX88_000447"
     CDS             44515..44922
                     /locus_tag="RHX88_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV2229181.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDSADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(45043..46035)
                     /gene="rpoS"
                     /locus_tag="RHX88_000448"
     CDS             complement(45043..46035)
                     /gene="rpoS"
                     /locus_tag="RHX88_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDV2229182.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(46098..47237)
                     /gene="nlpD"
                     /locus_tag="RHX88_000449"
     CDS             complement(46098..47237)
                     /gene="nlpD"
                     /locus_tag="RHX88_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDV2229183.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(47377..48003)
                     /gene="pcm"
                     /locus_tag="RHX88_000450"
     CDS             complement(47377..48003)
                     /gene="pcm"
                     /locus_tag="RHX88_000450"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDV2229184.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(47997..48758)
                     /gene="surE"
                     /locus_tag="RHX88_000451"
     CDS             complement(47997..48758)
                     /gene="surE"
                     /locus_tag="RHX88_000451"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDV2229185.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKTLREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGSRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPDTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(48739..49788)
                     /gene="truD"
                     /locus_tag="RHX88_000452"
     CDS             complement(48739..49788)
                     /gene="truD"
                     /locus_tag="RHX88_000452"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDV2229186.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(49785..50264)
                     /gene="ispF"
                     /locus_tag="RHX88_000453"
     CDS             complement(49785..50264)
                     /gene="ispF"
                     /locus_tag="RHX88_000453"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDV2229187.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(50264..50974)
                     /gene="ispD"
                     /locus_tag="RHX88_000454"
     CDS             complement(50264..50974)
                     /gene="ispD"
                     /locus_tag="RHX88_000454"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDV2229188.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(50993..51304)
                     /gene="ftsB"
                     /locus_tag="RHX88_000455"
     CDS             complement(50993..51304)
                     /gene="ftsB"
                     /locus_tag="RHX88_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDV2229189.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(51498..51821)
                     /locus_tag="RHX88_000456"
     CDS             complement(51498..51821)
                     /locus_tag="RHX88_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDV2229190.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(51871..52476)
                     /gene="cysC"
                     /locus_tag="RHX88_000457"
     CDS             complement(51871..52476)
                     /gene="cysC"
                     /locus_tag="RHX88_000457"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDV2229191.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(52476..53903)
                     /gene="cysN"
                     /locus_tag="RHX88_000458"
     CDS             complement(52476..53903)
                     /gene="cysN"
                     /locus_tag="RHX88_000458"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDV2229192.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(53905..54813)
                     /gene="cysD"
                     /locus_tag="RHX88_000459"
     CDS             complement(53905..54813)
                     /gene="cysD"
                     /locus_tag="RHX88_000459"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDV2229193.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            55065..56102
                     /gene="iap"
                     /locus_tag="RHX88_000460"
     CDS             55065..56102
                     /gene="iap"
                     /locus_tag="RHX88_000460"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDV2229194.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   56123..57005
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=56245..56273
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(57102..57395)
                     /gene="cas2"
                     /locus_tag="RHX88_000461"
     CDS             complement(57102..57395)
                     /gene="cas2"
                     /locus_tag="RHX88_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDV2229195.1"
                     /translation="MSMIVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(57392..58315)
                     /gene="cas1e"
                     /locus_tag="RHX88_000462"
     CDS             complement(57392..58315)
                     /gene="cas1e"
                     /locus_tag="RHX88_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDV2229196.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDLVVPKAFEIAARQPAEPDKEVRLACRDIFRSSKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(58312..58962)
                     /gene="cas6e"
                     /locus_tag="RHX88_000463"
     CDS             complement(58312..58962)
                     /gene="cas6e"
                     /locus_tag="RHX88_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDV2229197.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFTPELSTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSWQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(58944..59690)
                     /gene="cas5e"
                     /locus_tag="RHX88_000464"
     CDS             complement(58944..59690)
                     /gene="cas5e"
                     /locus_tag="RHX88_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDV2229198.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRCEELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDRFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(59701..60756)
                     /gene="cas7e"
                     /locus_tag="RHX88_000465"
     CDS             complement(59701..60756)
                     /gene="cas7e"
                     /locus_tag="RHX88_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDV2229199.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLEVAVQRITTLRENMNTVYEQKTDYASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(60768..61307)
                     /gene="casB"
                     /locus_tag="RHX88_000466"
     CDS             complement(60768..61307)
                     /gene="casB"
                     /locus_tag="RHX88_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDV2229200.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            complement(61304..62866)
                     /gene="casA"
                     /locus_tag="RHX88_000467"
     CDS             complement(61304..62866)
                     /gene="casA"
                     /locus_tag="RHX88_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDV2229201.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWEDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELADAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(62964..65621)
                     /locus_tag="RHX88_000468"
     CDS             complement(62964..65621)
                     /locus_tag="RHX88_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDV2229202.1"
                     /translation="MTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCF
                     GLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEK
                     ISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGQPPDRMDELD
                     NFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGS
                     STRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQ
                     QKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANA
                     MYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGC
                     AAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYM
                     SCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQEAPFLEKDDYPWL
                     THVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDA
                     IKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQ
                     VIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILA
                     PVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGE
                     DVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESV
                     SLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQ
                     DPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT        15477 a        17942 c        17001 g        15240 t
ORIGIN      
        1 atcaaaataa cacttttaaa tctttcaatc tgattagatt aggttgccgt ttggtaataa
       61 aacaataaat cctgaaggag agaacaatga tagaaaccat tactcatggt gcagagtggt
      121 ttatcgggct gttccaaaag ggcggagagg tgtttaccgg gatggtgacc ggcattcttc
      181 cgctgttgat tagcctgctg gttatcatga acgcactgat taattttatc ggtcagcatc
      241 gtattgaacg ttttgctcaa cgttgcgccg gtaaccctgt ttcccgttac ctactgttac
      301 cgtgcattgg cacgtttgtc ttttgcaatc cgatgaccct aagcctgggt cgctttatgc
      361 cggaaaagta caaacccagc tactacgcgg cggcctctta tagctgccac tcaatgaatg
      421 gcctcttccc ccatatcaac cctggcgaac tgtttgttta tcttggcatt gccagcggtc
      481 tgacaacgct gaacctgcca cttggcccac tggcggtgag ttatctgctg gttggtctgg
      541 tcaccaattt cttccgcggc tgggtgaccg atctgaccac cgccattttt gagaaaaaga
      601 tgggcattca acttgaacaa aaagttcacc tggcaggagc aacatcatga cgcatattcg
      661 gatcgaaaaa ggaacgggtg gctggggcgg cccgcttgag ctgaaagcca cgccgggaaa
      721 aaaaatcgtc tatatcaccg ccggtacccg gcctgcgatt gttgacaaac tggcacagct
      781 tactggctgg caggctattg acggatttaa agaaggtgaa cccgcggagg cggaaattgg
      841 tgtcgcggta atcgactgtg gcggcacatt acgctgcggc atctatccga aacgacgtat
      901 tcccaccatt aatatccact cgacgggcaa gtccggtccg ctggcgcagt acattgtgga
      961 agatatttat gtctctggcg taaaagaaga aaacatcact gtagtgggtg atgcgacacc
     1021 acaaccctct tccgtgggcc gtgactatga caccagtaag aaaatcaccg aacaaagcga
     1081 tggtttactg gcgaaggtgg gaatgggcat ggggtccgcc gttgcggtgc tgtttcaatc
     1141 tggtcgtgac accatcgaca ctgtattaaa aaccattctg ccgtttatgg cattcgtctc
     1201 ggcgctcatt ggcatcatta tggcttctgg ccttggtgac tggattgccc acggtcttgc
     1261 tccgctggcg agccatccac tgggtctggt catgctggcg ctcatctgct ccttcccact
     1321 gctttcacct ttcctcggcc caggcgcagt tatcgcacag gttatcggcg tattgattgg
     1381 cgtgcagatt ggtctcggca atattccgcc gcatctggct ttaccggcac tgtttgccat
     1441 caacgcgcag gcggcctgcg acttcatccc ggtcggtttg tcgctggcgg aagcccgtca
     1501 ggacacggtt cgcgtcggtg tcccttctgt actggtgagc cgctttttaa ccggcgcgcc
     1561 gactgtactg atcgcctggt ttgtctccgg ttttatctat caatagaggc tgaaacatga
     1621 ccgttattta tcagaccacc atcacccgta tcggcgcgag tgccactgac gccctcagcg
     1681 accagatgct catcaccttt cgtgaaggcg cgccagcgga cctcgaagag tattgcttca
     1741 ttcattgcca cggcgagttg aaaggtgaac tccatcctgg tttgcaattt acgctcgggc
     1801 agcatcgcta tccggtgacc gccgttggca gcgtggcgga agacaacctt cgcgaactgg
     1861 gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga atttccgggc actgtccatg
     1921 tggcaggccc tgtacccgac gatatcgcgc cgggatcggt tttgaagttt gaatctgtta
     1981 aggagtaaaa aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt
     2041 tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg
     2101 acaaagccgc aaatgtggca caagaaatta acgccgaata tggtgaaagt atggcgtacg
     2161 gttttggtgc tgacgccact agcgagcaaa gcgttctggc gctctctcgt ggggtagatg
     2221 aaatctttgg tcgcgtggat ttgctggtct atagcgccgg aatagcaaaa gcagccttta
     2281 tcagcgactt ccagctcggc gattttgacc gttcgctaca agtgaatctg gtgggttatt
     2341 tcctgtgtgc ccgtgaattt tcgcgtttga tgatccgcga cgggattcag ggtcgcatta
     2401 ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc tacagcgcag
     2461 cgaaatttgg tggcgtcggg ctgactcaat cactggcgct ggatctggcg gagtacggca
     2521 ttacggtgca ttcactgatg ctcggtaacc tgctgaaatc gccgatgttc cagtcactgt
     2581 tgccacaata cgcgaccaag ctgggtatca aaccggatca agtcgagcag tattacatcg
     2641 acaaagtacc gctcaaacgc ggctgcgatt atcaagatgt gctgaatatg ctgctgttct
     2701 acgccagtcc taaggcgtcg tactgcaccg gacagtcgat caatgtcacc ggcggtcagg
     2761 tgatgttctg atcaacagcg gagatccatt aaggatctcc gtgagactat agaatgcctg
     2821 atgcgctacg ctcatcaggc atacaggact tccgccacta cattaaggaa aagttatggt
     2881 atccgcactc atcaccgtcg ccgttatcgc ctggtgtgcg caactggcct taggcggctg
     2941 gcaaatttct cgttttaacc gtgccttcga cacactatgc cagcaagggc gggttggcgt
     3001 gggccgttcc agcgggcgct ttaaaccgcg ggtcgtggtc gccatcgcgc tggacgatca
     3061 gcagcgcatc gtcgacacct tgtttatgaa aggactgacc gtcttcgccc gaccgcaaaa
     3121 aattcccgca attaccggca tgcatgcggg tgatttacag cccgatgtga tctttcccca
     3181 tgatccacta tcacagaatg ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg
     3241 ttgtgaatgt tacttgcttg cgaagttatc attttgaaac ctaaatcagg taatcacgcc
     3301 catgaaacct cgtcagcgtc aggccgccat tctggagtat ctgcaaaagc agggtaaatg
     3361 ctcggttgaa gaattggcgc aatactttga caccacaggc acaaccattc gcaaagatct
     3421 ggtcattctg gaacatgccg gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa
     3481 agaggaatcc gatccgccta tcgatcataa aacactcatc aacacccaca agaaagagct
     3541 gattgcagaa gctgccgtta gttttatcca tgatggcgat tcgatcattc ttgatgctgg
     3601 cagtaccgtt ttgcagatgg ttcccctgct ctcgcgcttt aataacatca cggtgatgac
     3661 caacagcctg catatcgtca atgcgctatc cgaactggat aacgaacaaa ctatcctgat
     3721 gccaggcgga acgtttcgta aaaaatcggc ctcatttcac ggacagctgg cagagaatgc
     3781 cttcgaacat ttcagtttcg ataaattgtt tatgggcacc gacggcatcg atctcaatgc
     3841 gggcgtaacc accttcaacg aggtttatac cgtcagtaag gcaatgtgca atgccgcgcg
     3901 cgaagtgatt ttgatggcgg actcatcaaa gtttggccgt aaaagcccca acgtagtttg
     3961 cagtcttgaa agcgtcgata agctgattac cgacgcaggt atcgatccgg cgtttcgtca
     4021 ggcgctggaa gagaaaggga tcgatgtgat cataaccgga gagagcaatg agtgaagcac
     4081 tacagaacgc gggacgtcag acgttaatgc tggagttgca ggaagcaagc cgtttaccgg
     4141 aacgtctggg cgatgatttt gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag
     4201 tggtggtttc gggaattggc aaatcgggcc acattggtaa gaaaatcgcc gcaacacttg
     4261 ccagtaccgg cacaccggct ttttttgtcc atccggcaga agcactgcac ggcgatctgg
     4321 ggatgattga aagccgcgat gtgatgctgt ttatctctta ctccggtggc gcgaaggaac
     4381 tggatctgat tattccgcgt ctggaagata aatctatcgc gctgctggcg atgaccggca
     4441 aaccgacgtc accgctgggc ctggcggcaa aagcggtgct ggatatctcc gtagaacgcg
     4501 aagcctgccc gatgcacctt gcgccgacct ccagcaccgt caataccctg atgatgggtg
     4561 acgcgctggc gatggcggtc atgcaggcgc gcggatttaa tgaagaagat tttgcccgct
     4621 cccacccagc cggggcactg ggcgctcgct tgctgaataa agtgcatcat ctgatgcgcc
     4681 gtgacgatgc catcccacag gtggcgttaa ccgccagcgt gatggatgcg atgctggaac
     4741 tcagccgcac cggtctgggg ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg
     4801 tctttaccga cggcgattta cgtcgctggc tggttggcgg cggcgcactc accacgccag
     4861 ttaatgaagc gatgacggtc ggcggcacca cgttgcaatc gcaaagtcgc gccatcgacg
     4921 ccaaagagat cctgatgaag cgcaaaatca ctgccgcacc ggtggtagat gaaaacggca
     4981 aactcaccgg cgcaattaac ctgcaggatt tctatcaggc cgggattatt taatccttca
     5041 ttcccagacg tttcgccagc cgatgcaggt tggcgacgtc ggtttccagc atccgcgcgc
     5101 aggcagccca gttgtgatga ttttgtgcca gcgcctgacg aatagtttca cgctggaacg
     5161 cttctgtcgc ttcacgcagg ttttgcttaa caacgggcac cgccgccgct tctggcggcg
     5221 gcaacgtcac ctcaggaaaa gcaaaatgtt gcgcctcaag aatcacttca tcgccgttgc
     5281 gggtggctct cgacagcact accgcccgat gaatagcatg ttccagttcg cgcacgttcc
     5341 ccggaaaacg atagtgttgc agtaaatttc gcgctccggc acttaatacc acgcgggaga
     5401 gccccagccg caaacgacac tgctcgcaga aataccccgc cagcagaatg acatcatcgc
     5461 cccgctcacg cagcggcggc accgaaagtg gaaacacgct caggcgatga aacaagtcag
     5521 cgcgaaatcg ccctgccagc acctcttcgc gtaagtcgcg gttagtcgcc gccagcacgc
     5581 gcacatcgac ccgcaaactg cggtcatcgc caacgcgctg aatatcgcca tactgcaaca
     5641 ccctcagcag cttggcctgc aatgccaacg acaactcgcc gatctcatcc agaaacagag
     5701 tgccgttatc cgccatttca aacttcccgc tgcggttact gatagcgcca gtaaacgctc
     5761 ctttcacatg cccgaacagt tcactttccg ccacactttc cggcagtgcg gcacagttga
     5821 gatagaccag cggattcacc gcccgtggcg aggcttcatg aatcgctttc gccaccagct
     5881 ccttaccggt tcccgtctca ccgctgatca ggacgttgag atcggacgcc gccacaatct
     5941 caatctcttt tttcagttgc gtcatgccag gggacaagcc aatcatctgc gtctgtttca
     6001 ccgcttcaaa cggcgtggca tcgcctggca tcatattctg gctttccagt tgttcaatca
     6061 gcaacgcatt gcttaacgct cccgccgcca gcgcagcaat cagccgtagc tcttcgtcgc
     6121 tgaaaacatc gaactgatcg ggctgcatcc cgtcgagcgt cagtgcgccg atcaggtttt
     6181 gcccggcaaa caatggcaga ccaacgcagg cgtgaacctt cagactctcc tgcccaggaa
     6241 tcaaaccgtc atagggatcg ggcaattcgc tgtctgcggg aaagcgcacc acatccccgg
     6301 cgcgggcaat cgcttccagc cgtggatgcc cttccagcgc aaagcgtcta ccgagtacat
     6361 cctttgccag accgtcgatg gcaagcggaa taaactgccg cgaatcgtaa cgtagcaacg
     6421 cagacgcatc gcactccagc acctgacgta gcgtggtgat caggcgctga aaacgatcct
     6481 gatgaccaat cccacgctgc aattcgatgg cgatattcgc cagcacatca acggaaaaac
     6541 tcatctttgc ctcactgtca atttgactat agatattgtc atatcgacca tttgattgat
     6601 agtcattttg actactcatt aatgagcata cttttattta tatagtaaaa acaatcagat
     6661 aaaaaactgg cacgcaatct gcaattagca agacatcttt ttagaacacg ctgaataaat
     6721 tgaggttgct atgtctattg tggtgaaaaa taacattcat tgggttggtc aacgtgactg
     6781 ggaagtgcgt gattttcacg gcacggaata taaaacgctg cgcggcagca gttacaacag
     6841 ctacctcatc cgcgaagaaa aaaacgtgct gatcgacacc gtcgaccata aattcagccg
     6901 cgaatttgtg cagaacctgc gtaatgaaat cgatctggcg gatatcgatt acatcgtgat
     6961 taaccatgca gaagaggacc acgctggggc gctgaccgaa ctgatggcgc aaattcccga
     7021 tacgccgatc tactgtactg ccaacgctat cgactcgata aatggtcatc accatcatcc
     7081 ggagtggaat tttaatgtgg tgaaaactgg cgacacgctg gatatcggca acggcaaaca
     7141 gctcattttt gtcgaaacac caatgctgca ctggccggac agcatgatga cttacctgac
     7201 aggcgacgcg gtgctgttca gtaacgatgc tttcggtcag cactactgcg acgagcatct
     7261 gttcaacgat gaagtggatc agacggagct tttcgagcag tgccagcgtt actacgccaa
     7321 tatcctgacg ccgttcagcc gcctggtaac gccgaaaatt accgagatcc taggctttaa
     7381 cttaccagtc gatatgatag cgacctccca cggcgtggtc tggcgcgata acccgacgca
     7441 aattgtcgag ctgtacctga agtgggccgc tgattatcag gaagacagaa tcaccatttt
     7501 ctacgacacc atgtcgaata acacccgcat gatggctgac gctatcgccc agggaattgc
     7561 ggaaaccgac ccacgcgtgg cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga
     7621 aatcctgacc aatgtcttcc gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa
     7681 cgtgatgatg ccgaaaatcg ccgggctggt ggaggagatg actggtttac gcttccgtaa
     7741 caaacgcgcc agtgctttcg gctctcacgg ctggagcggc ggtgcggtgg atcgtctttc
     7801 cacgcgcctg caggatgcgg gtttcgaaat gtcgcttagc ctgaaagcca aatggcgacc
     7861 agaccaggac gctctggagt tatgtcgtga acacggtcgc gaaatcgccc gtcagtgggc
     7921 gctcgcgccg ctgccgcaga gcacggtgaa tacggtggtt aaagaagaaa cctctgccac
     7981 cacgacggct gacctcggcc cacggatgca gtgcagcgtc tgccagtgga tttacgatcc
     8041 ggcaaaaggc gagccaatgc aggacgttgc gccaggaacg ccgtggagtg aagtcccgga
     8101 taacttcctc tgcccggaat gctccctcgg caaagacgtc tttgaagaac tggcatcgga
     8161 ggcaaaatga gtaacggcat tgtgatcatc ggttcgggct tcgccgcccg ccaactggtg
     8221 aaaaatattc gcaaacagga cgccactatt ccattaaccc tgattgccgc cgacagcatg
     8281 gatgagtaca acaaacctga cctcagccat gttatcagtc aggggcaacg tgccgatgac
     8341 cttacccgcc agacggcggg tgaatttgcc gagcagttta atctgcacct gtttccacaa
     8401 acctgggtga cggatatcga tgccgaagcc cgtgtggtga aaagccagaa taatcagtgg
     8461 caatacgaca agctagtact ggcaaccggt gccagtgcct ttgtcccgcc tgtgcctggg
     8521 cgtgagttaa tgctgacgtt aaatagtcag caagagtatc gcgcctgtga aacgcaactg
     8581 cgggatgccc gacgcgtgtt gattgttggc ggtggtttga ttggtagcga actggcgatg
     8641 gatttttgtc gtgcaggcaa agcggtcacg ctaatcgaca acgctgccag tattctggcg
     8701 tcattaatgc caccggaagt aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt
     8761 catctgttgt tgaaatctca gttgcagggg ctggaaaaaa cagattctgg cattctggca
     8821 acgctggacc gccagcgcag tatcgaagtg gatgcggtaa ttgccgccac cggactgcgc
     8881 ccggaaaccg ccctggcacg acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat
     8941 agttatctgc aaaccagtaa tgccgatatt tacgcgctgg gcgattgcgc ggaaattaac
     9001 ggtcaggtat tgccgttcct ccagccgatt caacttagcg cgatggtgct ggcaaaaaat
     9061 cttctcggca ataacacgcc actgaaacta ccggcgatgc tggtgaaaat caaaacgccg
     9121 gaattaccgc tgcatctggc aggcgaaacc cagcgtcagg atttacgctg gcaaattaat
     9181 accgaacgcc agggaatggt ggcgcgcggc gttgacgatg ctgaccagct tcgcgccttt
     9241 gtggtcagtg aggatcggat gaaagaggca tttggattgt tgaaaacatt gcctatgtag
     9301 gtgggctact gtgcctaaaa tgtcggatgc gacgctggcg cgtcttatcc aacctacggg
     9361 gagcgcatgt gttggtcaca tactgcgttc atgtcgcatc cagctactgt gcccaaaatg
     9421 tcggatgcga cgctggcgcg tcttatccga cctacgggga cgcatgtgta ggccggataa
     9481 ggcgtttacg ccgcatccgg caatggtgtc caaatgcaac acgttttatc cgttctggac
     9541 ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc cccagagaca aaccgccatc
     9601 acccgccggt aaactctgtg gaaagagcaa tgtgaaatca gcgagataat gcgccagacg
     9661 tgcacgcagc aaacggttat gaataacccc gccgctaaat accagcgtag tgataccacg
     9721 catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat catgaaacgc
     9781 ccacgcgcgt tgattaaccg gtgcctgcca gttcagccac tgctgccaga aagtggcgag
     9841 atccagttga ttgtccacca gcggcattgt caccggatgc gtcactccgt ggcacgaagc
     9901 tgcgagtgcc tccagagcac aagccgcttc accttcataa cttaacgtgg ctggcgcaca
     9961 gcccagtgcc gccgccacgg catcgaacaa ccgcccacac gatgacgcca gcggcgcgtt
    10021 aattccacgc tcaatggccc gcgccagcac gctccagttt tgctgttgca cacttgctgt
    10081 ttcagaataa ttctgccact ccggcacaaa gcgcaggcac tgcgccagca ggtttcgcca
    10141 cggctgcttc gccgccaaat cgccacccgg aagcgccact gcaggcaagc cgcccaggtg
    10201 ctcacattcg cgatagttca cccgcagaca ctcgccgccc cacaaagcgc cgttctcccc
    10261 cataccgata ccatcgagcg tcaaagcaat gacatcaccg ccatccagcg gccactgatg
    10321 ctctgccaga cacgccgctg cgtgggcatg atgatgcagc accgtttgcg tcggcagatt
    10381 catttcgcgc gcccactggc tggagacata gcccggatgc acgtcatgca caacgtattg
    10441 cggagtgaaa tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact gcatctggat
    10501 gccatcgtca cttaaatcgc ccagatgctg actcaacacc gcttgttcac cgcgcaccag
    10561 gcagaaggtg tttttcagat ccgcgccaag acacagcaca ggcggaacat ttttaaagcc
    10621 cggaggcaaa accagcgcat ccggcacata cccgcgtgaa cggcgcagca tttcgccgct
    10681 ttcgcgcacc accgaatcat ccatccgctg cacgatgtcg cggttatgta tcaagaatcc
    10741 gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg ctgatagctg gtggtttacc
    10801 gctcaggttg ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca gcaaatgctg
    10861 gagcgggttc gcaggcaaca ttaccccgac ttcgttaagg tcaggggcga tatcatcaca
    10921 aagctcagga acgtattttt tatccaccag cacaatcggc gcggcgggcg tggtaagcaa
    10981 ctggcgcgca gcgtctggta aaccgtcagc cactggcaac atgaccgcca gcggtttcgc
    11041 cgggcgatgt ttgcgcgccc gcagtgtcgc caccgcgtta ctgttacgtg catcgcaggc
    11101 aagatgaaat ccgccaatcc ctttgatggc gacaatgttg cccattttta actgtgcgat
    11161 agccgcctgt aatgccgcct cttgttctgc atgttcacca tgacttaccc attcaagatg
    11221 tgggccacac tccgggcagg ccaccggctg ggcgtggaag cgacgatcga gcgggtcgcg
    11281 gtactctttg tcacaagccg gacacaacgg aaacgccgcc atcacggtaa acgggcggtc
    11341 gtaaggcatg gcgcgaataa tggtgaaacg cgggccgcag tgggtacagt tgataaacgg
    11401 ataacgataa cgccgttcgc ctggggtatt catttcggca agacaggcag ggcaagtagc
    11461 ggcatccggg acaatttgcg tattcatggc acctcccgcg ctctggcgga tagtgaactc
    11521 ggtgggcagt tgtgaccaga tgtacggctc acgctcgacg ctatcaatac gcgccagcgg
    11581 cgggcagtgc tgatgcaatt gaacaagaaa cgtttccggg tcttccagca gccggacttc
    11641 tacgccatcg ccgtcattac agacatcgcc gtgaagattt aattgctgtg ccagctgcca
    11701 gacaaacgga cgaaaaccga cgccctgcac tttgccacga atacgcagtt ggacaccgca
    11761 agatgtgttt tttgccattg agttattccc gccatcatga attgcgtaac ccgccctgcc
    11821 ggacacgact gcgtcgtatc cggcagtctc aggtcggcga taccgccagc tccgtattct
    11881 acgaatattt ccgggaattc ctttgatgcc agaacagttc tataagattt ttagaacatc
    11941 agcgccgtac ggcgacgttt ttctgcgctc agttgttcaa gtttattacg atcgacacaa
    12001 atcagcgcat gggtcgggca agccgccata cacgccgggc cgtcttcgcg atggttgcac
    12061 aggtcgcatt tattggcttc agctttgtca gcccgtacat tcagccccgc gccgctgttg
    12121 cggatcaccg gacgtaccac cacttccatc gcgccatacg ggcaagccac cacgcaggtt
    12181 ttgcaaccaa tgcaacgttc ctgcatcaca tgaacaaacc ctttatcacg gctgatagca
    12241 ccattcgggc agacgttagc gcacggtgca tcttcacact gacggcagac tgtcgccgtg
    12301 gaaatgttca cacctttaat gacatggatt cgcggtaaaa aggtttccgg ggtcagcgat
    12361 gcacagtcct gattttcctg atgagaaacc acgcacgcca cttcacaggt acggcaacca
    12421 atacatttac tcgcgtcagc aatgatgaaa cggttcatca aattctccag caatgacagt
    12481 taatgcgccg atacattcac aaatcatgcc agtttttaat ttgctgttat ttaaggaaat
    12541 taatttctgt aatgcaggaa aaaagatgtc atcgacacta gtgacgatga catgtgatga
    12601 caatgtttat cgcgaaaggg caatgagtga gtcgcggcgg atcagttttc cgctgaaggt
    12661 tttcggcggt gagaaatccc cgccatcgag cataaaaatc agccgtccaa taatttcctg
    12721 aatcatctct gttaccggaa tttttacgct ggagagcgcc ggaacggtgt agggggcaat
    12781 tgcgatatca tcgaatccga taaccgacac ctgctctggc accgctacgc cgcgctcgtg
    12841 caacgctttg atcgcaccta tcgccatatc gtcgttactg gcaactaacg cgctaaattt
    12901 agccccacgt tcgagcaacg tttctacccc ttcggccccg ctggcgggcg tccatttacc
    12961 gttagcgata agtttttcat tgagcgcaat accatgctgc gacagcgcgt ctttataccc
    13021 ggcaagacgt tcaacgctgg tgggggaatc catcgagccg gtaaggaaag caatctcctg
    13081 atgtccggcg tttatcaact ctgccacggc attaaagctg gtctgtttat gatcgcacca
    13141 gacgctatgg ctgctgtttt tgcgcaggcg gcgattaagc accattatcg gctgactgtg
    13201 cgcgtcaatg atgtcatcaa tctcatccac gcttaaaaaa cgcgggtaaa tcataatggc
    13261 gtcgcagcgc aaatccagca gatactgaat cgcctggcgc tcttcttcgg cgctgtgttt
    13321 accatctgcc aatagcaact gccgcccttt ctcttccgcc attcgcgcgg catgaaacag
    13381 taattcacta aaataaatgc cgtgataaag cgtgttggtc actaccagcc ccagcgtctg
    13441 agtactcttc gccgacagat tgcgggccag caaatttggg cggtaaccgc tctcttctac
    13501 cgcctgaaac acgcgatctt tagtctcctg gctgacgtag ccattacctg aaagcacgcg
    13561 ggaaacggtc gcttttgaaa ccccggcgcg cttcgccact tccagcatcg tcgtcatcat
    13621 tttcatccct ttacacgcaa tcaacgcagt gtactgcacc gtttgccgat tgtccttgcg
    13681 caatcggcgg gaaaaatatt caggtgaccg gtttcacaaa tataaaaaat gaacaattaa
    13741 ctctcttgct tatttagtga caactattca tgattttgtg aaaccggttt cctaattccg
    13801 tttcagcatc ggcatttttc cgttacgtcg actgataaca actacatcta ccctactgat
    13861 aacaggataa aatccgatgg ccaaaaatta tgcggcgctg gcacgctcgg tgatagcggc
    13921 actgggcggc gttgataaca tctcggcagt cacgcactgt atgacgcgtt tgcgctttgt
    13981 tatcaaagat gaccagctta tcgacagccc gacgttaaaa accatccccg gcgtgctcgg
    14041 cgtggtacgt agtgacaacc agtgtcaggt gattatcggc aataccgttt cacaagcctt
    14101 tcaggaagtc gtcagcctgc tgccgggaga tatgcagccc gtacagcccg tgggtaaacc
    14161 caaactcacg ctgcgtcgca ttggtgcggg gatcctcgat gcgctgatca gcaccatgtc
    14221 accgctgatc ccggcgatta tcggcggatc gatggtcaaa ctgctggcga tgatcctcga
    14281 gatgagcggc gtgttgacaa aaggatcgcc gaccttaacc attctgaatg tgattggtga
    14341 cggtgctttc ttcttcctgc cgctaatggt cgcggcatct gccgccatca aatttaaaac
    14401 caatatgtcg ctggcgattg ccatcgcagg tgtgctggta catccgagct ttattgaact
    14461 gatggcgaaa gcggcccagg gtgaacatgt cgaatttgcc ctgattccgg tcaccgcggt
    14521 gaaatatacc tacacggtga tcccggcgct ggtgatgacc tggtgcctgt catatatcga
    14581 acgctgggtg gatcgcatta caccggctgt gaccaaaaac ttcctcaagc caatgctgat
    14641 tgtgttgatt gccgcaccgc tggcaatcct gctgattggc ccgattggta tctggatcgg
    14701 tagcgccatt tcggcgctgg tttacaccat tcatggttat ctgggctggc tttcagtcgc
    14761 cattatgggc gcgctgtggc ctctgctggt aatgaccggg atgcaccgcg tctttacgcc
    14821 aaccatcatt cagaccattg ccgaaaccgg caaagaaggg atggtcatgc cgtcagagat
    14881 cggcgctaac ctgtcgctgg gcggttcatc actggcggtg gcgtggaaaa cgaaaaaccc
    14941 ggaactgcgc cagacggcgc tggctgcggc ggcatcagcc attatggcgg ggatttccga
    15001 accggcgtta tacggcgtgg cgatccgcct gaaacgtccg cttatcgcca gtcttatcag
    15061 cggttttatt tgcggcgcgg ttgccggtat ggcggggctt gccagccact caatggcagc
    15121 gccggggtta tttaccagcg tgcaattctt cgatccggcg aatccaatga gcatcgtctg
    15181 ggtgttcgcg gtcatggcgc tggcggtggt gctgtcgttt atcctcacac tattgctcgg
    15241 ctttgaggat attcctgttg aggaagcggc tgccgaggcg cgaaagcatc agagcgcaca
    15301 accgaccgtc gccaaagaag taagtcttaa ttgaggatga aaatgtcagt atttccagaa
    15361 ggttttttat ggggcggcgc gcttgccgcc aaccagtctg aaggtgcgtt ccgtgaaggt
    15421 gacaaaggac tgaccacggt cgatatgatc ccgcacggcg agcatcgaat ggcggtgaaa
    15481 ctggggctgg aaaaacgttt tcagttgcgc gatgacgagt tttatcccag ccatgaggcg
    15541 acggattttt atcatcgtta taaagaagat atcgccctga tggcagagat gggattcaag
    15601 gttttccgta cctcaattgc ctggagccgc ctctttccgc agggcgatga aatcacgccc
    15661 aatcagcagg gcattgcttt ttaccgttct gtctttgaag agtgtaaaaa gtacggtatc
    15721 gaaccgctgg tcacgttgtg ccacttcgat gtgccgatgc atctggtcac cgaatatggc
    15781 tcctggcgta accgcaagct ggtggagttt ttcagccgct acgccagaac ctgctttgaa
    15841 gcatttgatg gtctggtgaa atactggcta accttcaatg aaatcaacat tatgttgcat
    15901 agcccgttct ccggcgcggg tctggtgttt gaagaaggtg aaaatcagga tcaggtgaaa
    15961 tatcaggccg cgcatcacca gctggttgcc agtgcgctag ccaccaaaat cgcccatgag
    16021 gttaacccgc aaaatcaggt gggctgtatg ctggcgggcg gtaacttcta cccttacagt
    16081 tgcaagccgg aagatgtctg ggcggcgctg gagaaagacc gggaaaacct gttttttatc
    16141 gatgtgcagg cgcggggcac gtatccggct tactctgccc gcgtattccg cgaaaaaggg
    16201 gtaaccatca acaaagcacc gggcgatgat gaaatcctga aaaacaccgt cgattttgtc
    16261 tctttcagct attacgcctc gcgctgcgcc tcggcggaga tgaacgccaa caacagcagt
    16321 gcggcgaacg tggtgaaatc gctgcgtaat ccgtatctac aggtgagcga ctggggctgg
    16381 ggaattgatc cactcggtct gcgtatcacc atgaatatga tgtacgatcg ttatcagaag
    16441 ccgctgtttc tggtggaaaa cggcctcggc gcaaaagatg aacttgctgc caatggcgag
    16501 attaacgatg actatcgcat cagctattta cgcgaacata tccgcgcaat gggcgaagcg
    16561 attgcagatg gcattccgct gatgggctac accacctggg gctgtattga tttagtttcc
    16621 gcctctacgg gtgaaatgag caaacgctac ggttttgtct acgtagaccg tgacgacgca
    16681 ggcaacggca cgctgacgcg cacgcgtaag aaatcgttct ggtggtataa aaaagtaatt
    16741 gccagtaatg gggaagattt agagtaagta accgtgccgg atgcggcgtg aacgccttat
    16801 ccggcctaca tagttcgtgc gacatgtagg cccgataagc gcagcgcatc gggcaatctg
    16861 gggtttggca tcagtcaaaa tcaaagaaac cattccggct taacgccaaa tcgggctgaa
    16921 agtgccttaa tgtgggatat tgtcagggat cgttgtccag acaaaatctg actgaccagg
    16981 gatttagagc cgatttcgtt cttcaaatcc gcataggtca gattatgttg atcaatcaac
    17041 gtgcgaagta acgccacgcc aaccggcatt gccgcaacgg ctttgtcgaa ctctgcaaac
    17101 ttttcgttgt tgttttcata ctctgcaatg cggctcgcca gaaagtcgat aagtggattg
    17161 gtgtcatctt cttcaatcaa atattcgacc agttccagtg cttcacgata atcatcttct
    17221 gagtcactgc ctcccagaaa ggggactgca ttgaccagtt ctcttgttgc tttaacagcg
    17281 cgtgcagcat tagcagtcat tctttgttct ctctgtagta acgtgacgtc tgtctgcacg
    17341 taatccccct catagccact ctgcaaaaag tcagttctgg ttcaccccat aaagcacatc
    17401 cccctttatc aagcaacgct gcgtctgcgc cagatcttcc gtttgcgggt cgcgcccggc
    17461 gatgggtttg aggacgagtt tacggttggg ggtataagta aaacgcgtga gcgcgggata
    17521 gcttaacttg ccaaaccagc cgtgaacacg ctgccatttg gcgtctttta cctgatacag
    17581 actggttatc cagtaaccgg aatcaaactg catatccagc aattgcgtgt ggccattcct
    17641 ttgtaaatcc agcaaatcat caatgccagt ttcacttgcg gtataaaaac catcggactg
    17701 aaaaacacac gggcgacctt gctgattaaa ggtcatcaga atcaaaaacg cgttaggtgc
    17761 cagaccattt cctgaaatac cccgccagat aaccagatcc tgaatgccgt tacgatcaag
    17821 atcggcacgg taaagttgcg cattacccgc catattttgc agcaccccca caggcacaga
    17881 ccaggattta ccggcgcgat ctttaccgtt caattgccat tcactatcat catttggcat
    17941 cttttcaagc gtcacgccag caacgcccac ttttctaaaa tgccaggttt gctcggcatc
    18001 ataaggcatg gtcaggagag gtttcagcgc ccgcttttgc gcgtattcgc tgtcactcat
    18061 tggcagatat tcggtaaagc tggtggcctg tgcaggcagc actaagctca ataaaaaagc
    18121 gcacatccct gttctgatat tcatttcgct gaattccctg atggagaatt attcttcttc
    18181 caccgctaac tgcgcgaagc cgccattccc ttcccagcct tccagtcgtt gataaacggt
    18241 ttctaccgca tctttaatcg gctgagtcat cgggtagtaa aagccgacga tatccggctg
    18301 gatgccgagg aaaatcactt cgccaatgtc ttctttcaac tggtcgataa ggtagttcaa
    18361 cggcatgtta tgggtagtca tcataaacat ctcggcgata tcatccgggt cgatgatgcg
    18421 gatttcgccg gggtttagcc ccatatcagt agcgtcaaca atcagtaacc gcgtagggcg
    18481 cagttcacgg atggcgacga tgtcgttttc cggtgcacta ccgccatcaa tcaccaccca
    18541 gttacctttc ggcgcggcgg cgcacttttc cgccagcagc gggccagcgc catcatcgcc
    18601 catcatgcta ttgccaacac agagtaaaac gtcagtcacg cagtctcctc accatcaaat
    18661 agatggcgtt ttcctgatga atatcatgca gcatacttag cagcattgta ctccattcct
    18721 gctgctgcga agtttgcgct gcccgggctt tatcgaatgc gttggccagc atcggcacat
    18781 ggttaatgtc gatgacgatc tcaccatatt tcggcacgcc ttccattttg cggcgggctt
    18841 cgctgcccgc ctccagagtg gcaatccatg ccagatattc atcccacggg caggtgagcg
    18901 ccgcttccag gcaatcgata accccaaggt ggtgaccaat cgccaggctg taatagacca
    18961 cctgctgcgc ctcggcgggc gtggcatcgt tctcatcaat aaatttacgg ctcagttgac
    19021 tgaacaccac cttttcactc atcggatacg cgcctcttct acaacatgat tcagatggct
    19081 gacaatctcg ttcagacgcg gatcgttttc cgcttccagc cagcgtgcaa cctgttcttc
    19141 gccctgccct aactgtgtca ggaagtcatc ggcaatctga cgaccgtaac gataacctgc
    19201 caggcggcgt gcttcgcgat ccactttcac acgcagcggc tgcaccatat cgccatgcag
    19261 gatttccgcc ggttgttcat cctgctcacc cgggccacgg gcgtgaattt tctgctccag
    19321 caggccgagc gccattgcaa agccgtacag cgtggcggca ggcgttggcg ggcaaccagg
    19381 gatgtagaca tctaccggga cgattttatc cgtaccgccc cacacgcagt agagatcgtg
    19441 gaagatcccg ccactgttac cgcaggcacc gtaggagata cagattttcg ggtccggcgc
    19501 ggactgccac gcacgcagcg caggggatcg cattgcacgg gtgaccgcgc cggtaaacag
    19561 taaaatatcc gcatgacgcg gtgaaggaac gactttaatg ccgaagcgtt ctgcatcaaa
    19621 cagcggcgaa agcgtggcga aaatttcgat ttcgcagccg ttgcagccgc cgcagtccac
    19681 gcggtaaacg taggcagaac gtttgatttt tttcagtaac gacgccttca tgctggcgat
    19741 ggattcatcc accgtcatcg ggaccggaat gccgttggcg tcacgggggc ctaataaatt
    19801 gctcatcaga tggcctcttt catatggcga gtcagttcaa tacggtcgga cggcaccagg
    19861 catttctggc gcttacattc cgggcaagtc tcaaagcttt cgcggtggtt ttccgcgcgg
    19921 ctgtcaccat tgtgcttaag cagcgcaatg gcgtagtcga tctctttctg gacggcgaaa
    19981 ggacgattgc agacgcgaca gctgcacagc acaaaacggg actgttgcag gaaatcttct
    20041 ttcttccaca ccgccagttc gtactcttgc gacagtttga tcgctgccgt cgggcagact
    20101 tcttcgcagc gtccacagaa gatgcagcgc ccgagattaa actcccacgc aagctctcct
    20161 gtggcgaggt cagtttcaac cgttaaggcg tttgacgggc aggcattgac gcaggccgcg
    20221 cagccgatgc actgctgcgg gttctgctct ggcttaccac ggaagttttt atcaaccgca
    20281 atcggctcca gcggataaga cgaggtcgcc gtgccggttt tgatgacttt tttgataaag
    20341 gtaaacatgg cgattcctta tttcagcggc gagtttttac gctcaatgct gtaacgctcg
    20401 agttctttgt acggcaccac tttgctcttc ttcttacgca catcgaccac ggtcatgcgg
    20461 tcggtacagg agtagcaagg gtcaaggcta ccgataatca gcggcgcatc ggaaacggta
    20521 ttgccacgca gcatgtagcg cagggtcggc cagttcgcgt aggtcgcggc acggcagcgc
    20581 cagcggtaca gcttctggtt gtcgccggtc atgctccagt ggatatcgtc gccgcgcggc
    20641 gcttcggcaa agcccagcgc gaaacggtgc ggaatgtagg taaagccttc caccatcagc
    20701 ggaccgcccg gcaggttatc cagaccgtag tcgatcatgt tcagcgcggt ataaacttcg
    20761 ttgatacgca ctttcagacg ggaaataacg tcgcagccct gctcgctgtg gacttccatt
    20821 ggcagcaggc cataaccgac aaacgggtga tcggcgcgag tatcgcgggc gtgaccgctg
    20881 gcgcgaacca tcgggccaac gttactgaaa tcgcgggcga tttccgggtc cagacgacca
    20941 atgccgacgg tgcgctgttc catgttcggc gtactcagca gcacatccac cagctcctgc
    21001 acttcacgac gcatctgttg cgccagctgg cgggtctgga tcatgtcatc tttcaacaga
    21061 tcgcgacgaa tcccgccgat caggttcagg ccgtaggttt tacgcgcacc ggtgaggatc
    21121 tctgccattt tcatggaggt ttcacgcacg cggaagaact gcataaagcc ggagtcaaag
    21181 ccggtaaagt ggcaggcgag accgaggttg agcagatgcg agtgcaggcg ttccacctcc
    21241 agcagaatgg cgcggatcat ctgcgcacgt tctggcacct gaatacccat cgcgttttct
    21301 accgacgtgg tgtaggcggt gctgtgggca aagccgcaga tcccgcacac acggtcagag
    21361 aggaaggtca cttcgttgta acccatacgg gtttccgcca gtttttccat gccgcgatga
    21421 acgtagaaca gacggtagtc ggcgtcgata atgttttcgc catcgacgaa cagacggaag
    21481 tggcccggtt cgtcagaagt gacgtgcagc ggaccaatcg gcacgacgtt gtttttcttg
    21541 tcgcccagtt cgttgatgaa ctcgtaggtt tcagcatcgg tagtcggtgc cggacgctga
    21601 cgataatcca tgctgtcttt acgcagcgga taaagttcat ccggccagtc atccggcagc
    21661 accagacgac gttcatccgg caaaccaacc ggaatcaaac cgtacatatc gcgcacttcg
    21721 cgctcgcccc acaccgccgc cggaacgcgc ggcgtcacgg acggatactc cggtttgttg
    21781 gcgtcaactt cgacgcgaac cgttacccaa cacttggtgc ccttctccat cgacagcacg
    21841 taataaacgg cgtaatgacc attcagtttg cgttcgtcgt taccaaacag caccgacagc
    21901 cagccaccct gcttgtagta gagaaactcc accacttccg gcaggtagtt caccttcacg
    21961 gtcacggtca gctgatcttt ggtctgccag gcgtggtcca gcacgacgcc cggaaatgcc
    22021 tcattcagcg cggcgagata atgttgacct aatttttctt cagacatgct caaactctct
    22081 ttaatcacgc cgccagcaag gagacgaacg ctaaaaatgc aaagccaaac ccagcccagg
    22141 taatgcgcgg agtaatatca agacgcagac gggccatgct gttttcgaac agcgcgataa
    22201 ccaggacgcc gacgaccagt ttgacgatgg caatcaccag cgccagcagc agcccaccgg
    22261 cggtgaaggt ttccatctgt ccccacggga taaatacgcc gacgaacatc tgcagcacca
    22321 ccagctgttt caggctgata ccccatttca tgacgccaaa gccgctgccg ctgtattcag
    22381 agagcgggcc ttcctgcagc tcctgctcgg cttccgccag gtcgaacggc agtttgccca
    22441 tttcgataaa ggtggcgaac gcacaggcac aaagcgccag taccagcggg atgctctggc
    22501 tcagcggcca gtgataaacg gtgtcggtga tgttgctgat gttggtggaa ccggcaacct
    22561 gtgcggcaac ccacagacca agcagcagca tcggttcgac cagcacgcca agcatcgctt
    22621 cacggctcgc gccgatagcg gtaaacgggc taccggtatc cagaccagaa atggcaaaga
    22681 agaaacgcgc gatggcaaag agatacagta aggtgattaa atcacccagt tgcggcagcg
    22741 gagaaccgac ggtcaccacc ggcagcgcag tagcgatagt cagcatgacg cccaccatca
    22801 catacggcgt caggcggaac acccagccgg aggcatccgg gccaacgctc tggcggccca
    22861 gcagtttgat aatgtcacga tactcctgca acacacccgg cccacgacgg ttatgtaagc
    22921 gggctcgcgc cacgcgggta ataccggaga gcagcggcgc aacggcaaat agcaccagcg
    22981 cctgaattaa cggatataaa acactcattc tcaggctcct cgtgaaacaa taatcaccac
    23041 cagtaccgcc agttcaacca gcgccatccg gcggaacagc aacgcactcc cctcgcactg
    23101 ccagcccggc accagagaca ccggattcag ccatttgcgt agtttcagca ccggcgcaaa
    23161 cgcctgtttc accggcatgg caaaaccgtg agcggtaatc accattgatt tttcgtggtc
    23221 gtagccgcac acccaggccg cgccgcggga acgcgatggc aaacgatcgc ctttgcaaat
    23281 cgccataatg atgaatggca gcagcgggca ggcaatcagc agcaatgtga tcattggttg
    23341 agaaacggtg gtgttagcag gctccagcgg cagaggtaca gcagcagaca gcatcggcag
    23401 cagccacggc gcagcaacac cgccaattac gcagcaaatc gccagtgcca ctacgcttac
    23461 cctcatcagg agcggcgcac aggtggcgtt ttcggcttct ttggtacgcg gcgcgccgag
    23521 gaacgtgacg ccatagactt tcgccataca catcaccgcc agcgcaccgg taattgccag
    23581 acccacagcg agcagcggcc ccagcagacg agcaacaaac gcgccactat tgctcagttt
    23641 gaagaaggat tgatagataa cccattcccc ggcaaacccg ttcagcggcg gcagcgcagc
    23701 cattgccatc aacccgacta acatggcgat ggagataacc ggcattttct tgccaatacc
    23761 accgagtttt tcgatatcgc gatgaccggt acggaaccag acgctccccg ccccgaggaa
    23821 cagtacgctt ttgaacaggc tatggttaag cagatggtac agaccaccga ccagaccaag
    23881 ggcaatcagc gccggttgtt cgagcgcgat acccgttacg ccagcgccca gccccagcag
    23941 gataatgccg atattttcca gggtgtggta agccagcagg cgctggatgt tgtgctccat
    24001 cagcgcatac agaccaccga caaacgcggt gatcatgccg agcaccagca gcgcgatccc
    24061 ccaccacagc ggtgcattac cgcccagcag tgacagggtt aaaatgccca gcaggccaat
    24121 tttcatgact accgtagaaa acaacgcggc agctggcgca gaggcgttag catgtgcctg
    24181 cggcacccag ccgtgcagcg gaataatccc ggcgagcagg ccaaagccaa tcacaccgag
    24241 cagccagata tcggaaccga gcggcagctg ttgcatacgc atatccagca ggcgcagatc
    24301 cagcgtgcca taacgctgcc acagcagcca gcaagcaatc gccagcagca gagtgccaag
    24361 acgccccagc gcaaaccaca gtttgccctc tttgctgttg ctagtgagga acaccgcgca
    24421 cagggccatg atttcggcca ttaccacgaa catgccgagg ttgctggcaa tgacgacgca
    24481 gacggcggca gccatcaaca tattgatctg caagccgttg cacttcacct gcgcgtggcg
    24541 atgccagtca atgttataga ggctgacaaa cagaccgcaa aggccgaggg taatcagcca
    24601 aatcgcgtta agcggggaga tttgcacatc gtagcttacc agcgacagcg caccgctcac
    24661 gccaaccgcg ccagtcagca cagtgaagcc cgcggctgcc gtatacaggc taccaaccgc
    24721 gccgccaatt ccggctatcc agccacttag cgtcttttga aaagaaaaga gaaatgccag
    24781 aacagcggcg gcgacaaacc acgccacgcc gctattgatc agggaaattg cgctcattta
    24841 gcctctccac tttgagcctg ctgaaacaag gtgagatcgc caaagtccgt gttaaaggtc
    24901 agctcacgct tacgtttgct gacgcgggcg atatcggtgt tatccaccag atgcagggct
    24961 ttagtcgggc acatccgcac gcaggccgga ccttgttcat caaagctaca aaggtcacat
    25021 ttgacggcga tcgcgcgaat acctggcacc cagtcaagca atgtgctgac acgcgccgga
    25081 gcaggcggtg ccggtggcgc tttcggggta ttggcgtttg ccggaatatc cagcggacgg
    25141 ctgccggaaa attcaattgc gccaaacggg caggcgatgc cgcacagctt gcagcttacg
    25201 cacaggcttt cattcaactg cacggcccca tcaacgcggg tgatggcgtt aaccgggcag
    25261 accaccgcgc agggtgcatc ttcacagtgg tgacagagct gcggcgcaga ttctttttca
    25321 tttagcatca ctctcaggcg cggcattgat tgcaggccgt gctggcgatg cgtctctgaa
    25381 caggcggcct cacaagtgtg gcagccgata cagagcgtgg agtcagcaat tacaaaacga
    25441 ttcaccaggc attcctcagg tgattgtcat ttttgacgaa aacatgccga tgaaatgtca
    25501 ttttcgacac tcatcgacac gcccatcccc aaacaggcgt aacgcctgca aaacgggcaa
    25561 agcctcagct catgctgccg ggctttgtcc ctttaccagt tggcttaaat tcaccggcac
    25621 attgttttca atggcggtgt ataagctgtc gtaaacgcca gcgatttttt cgagatagtg
    25681 ttccagaacc tgttcgcgat cgcggatgtt gaccttgcca tcaatcatgc cgtcaatgct
    25741 cagttgcgcc tgcgcaatcc gctgtttgtc ttcgccatct tttgtttcga cgtaatactc
    25801 gatggtgtgg ctgttaaagc catccgccag ggtgacgtaa atacgaaaat gttcaaaaag
    25861 gacgaaacag agttctttgt ccggcgcgca gctcatggcg tgatgcaggc gcgctttcga
    25921 taacgtgatc ccctgaacca gcgaattgca gtagatgtgc cactggtcct gtaggcgacg
    25981 atgccgctgt gcgatgtaat cggctttctc gcttatttcc caaatagtca ttgtcaggtt
    26041 acccgtttaa cagagatgcc agctttaagc attttttgtg ccaactttta atttattgtt
    26101 attaaagaga tttttaagct aaagatgaat ttcgtcgccg tgtcgacgtg tcatttcgac
    26161 atcatcgaca ttattcaccg cagggataat caacactggc acaattattg cttgtagctg
    26221 gcaatagtta atgggaggcg atatgcacga aataaccctc tgccaacggg cactggaatt
    26281 gatcgaacag caggccgcaa aacacggcgc aaaacgcgta actggggtct ggctcaaaat
    26341 tggcgcattt tcttgtgtcg aaaccagctc tcttgccttt tgttttgatc tggtttgccg
    26401 cggcagcgtg gcggaaggtt gtaaactgca cctcgaagaa caagaggccg aatgctggtg
    26461 tgaaacatgc caacagtatg tgacgctact gacccagcgc gtccgccgct gtccacagtg
    26521 tcatggtgac atgctacaga ttgtggcaga cgacggttta cagattcggc ggatagaaat
    26581 agaccaggag tgagcgatgt gtacaacatg cggttgcggt gaaggcaacc tgtatatcga
    26641 gggtgatgaa cataaccctc attccgcgtt tcgtagcgcg ccatttgccc cggcggcacg
    26701 cccgaagatg aaaatcaccg gcattaaagc gcctgaattt acccccagcc agactgaaga
    26761 aggcgacctg cattacggtc atggcgaagc gggcactcac gcaccgggca tgagccagcg
    26821 tcggatgctg gaagtcgaaa ttgacgtgct ggacaaaaac aaccgtctgg ctgaacgcaa
    26881 ccgcgcgcgc tttgctgccc gcaagcaact ggtgctcaac ctggtttcca gccctggttc
    26941 cggtaaaacc accctgctga cggaaacctt aatgcgcctg aaagacagcg ttccgtgcgc
    27001 ggttattgaa ggtgaccagc aaaccgtgaa cgatgccgca cgcattcgcg ctaccggcac
    27061 accagcgatt caggtgaaca ccggtaaagg ctgccatctt gacgcacaga tgattgccga
    27121 cgccgcaccg cgtctgccac tggacgataa cggtattctg tttatcgaaa acgttggcaa
    27181 cctcgtatgc ccggccagct tcgatctcgg tgaaaaacac aaagtggcgg tgctttccgt
    27241 taccgaaggt gaagacaaac cgctgaaata tccgcatatg tttgccgccg cctcgctgat
    27301 gctgctcaac aaagttgacc tgttgccgta tctcaacttt gacgttgaaa agtgcatcgc
    27361 ctgcgcccgc gaagtcaatc cagaaattga aatcatcctt atttccgcca ccagcggtga
    27421 agggatggac cagtggctga actggctgga gacacagcga tgtgcatagg cgttcccggc
    27481 cagatccgca ccattgacgg caaccaggca aaagtcgacg tctgcggcat tcagcgcgat
    27541 gtcgatttaa cgttagtcgg cagctgcgat gaaaacggtc agccgcgcgt gggccagtgg
    27601 gtactggtac acgttggctt tgccatgagc gtaattaatg aagccgaagc acgcgacact
    27661 ctcgacgcct tacaaaacat gtttgacgtt gagccggatg tcggcgcgct gttgtatggc
    27721 gaggaaaaat aatgcgtttt gttgatgaat atcgcgcgcc ggaacaggtg atgcagttaa
    27781 ttgagcatct gcgcgaacgt gcttcacatc tctcttacac cgccgaacgc cctctgcgga
    27841 ttatggaagt gtgcggtggt catacccacg ctatttttaa attcggcctc gaccagttac
    27901 tgcctgaaaa cgttgagttt atccacggtc cgggttgccc ggtgtgcgta ctgccgatgg
    27961 gtagaatcga cacctgcgtg gagattgcca gccatccgga agtcatcttc tgtacctttg
    28021 gcgacgccat gcgcgtacca gggaaacagg gatcgctgtt gcaggcaaaa gcacgcggtg
    28081 ccgatgtgcg catcgtttac tcgccgatgg atgcgttgaa actggcgcag gagaatccaa
    28141 cccgcaaagt ggtgttcttc ggcttaggtt ttgaaaccac catgccgacc accgccatca
    28201 ctctgcaaca ggcgaaagcg cgtgatgtgc agaattttta cttcttctgc cagcatatta
    28261 cgcttatccc gacgcttcgc agtttgctgg aacagccgga taacggtatc gacgcgttcc
    28321 tcgcgccggg ccacgttagt atggttatcg gcactgatgc ctataatttt atcgccagcg
    28381 attttcatcg tccgctggtg gtggctggtt tcgaaccgct tgatctactg caaggcgtgg
    28441 tcatgctggt ggagcagaaa atagcggccc acagcaaggt agagaatcag tatcgtcggg
    28501 tggtaccgga tgccggtaac ctgctggcgc aacaggcgat tgccgatgtg ttctgtgtca
    28561 acggcgacag cgaatggcgc ggcttaggcg tgattgaatc ttctggtgta cacctgacgc
    28621 cagattatca acgattcgat gccgaagcgc atttccgccc ggcaccgcag caggtctgcg
    28681 atgacccgcg cgcacgttgt ggtgaagtat taacgggcaa atgtaagccg catcaatgcc
    28741 cgctgtttgg taacacctgt aatcctcaaa ccgcatttgg tgcactgatg gtctcctccg
    28801 aaggagcgtg cgccgcgtgg tatcagtatc gtcagcagga gagtgaagcg tgaataatat
    28861 ccaactcgcc cacggcagcg gcggccaggc gatgcagcaa ttaatcaaca gcctgtttat
    28921 ggaagccttt gccaacccgt ggctggcaga gcaggaagat caggcacgtc ttgatctggc
    28981 gcagctggta gcagaaggcg atcgtctggc gttctccacc gacagttacg tcattgaccc
    29041 gctgttcttt cctggcggta atatcggcaa gctggcgatt tgcggcactg cgaatgacgt
    29101 tgcggtcagt ggcgctattc cgcgctatct ctcctgtggc tttatcctcg aagaaggatt
    29161 gccgatggag acactgaaag ccgtagtgac cagcatggca gaaaccgccc gcacggcagg
    29221 cattgccatc gttactggcg atactaaagt ggtgcagcgc ggcgcggcag ataaactgtt
    29281 tatcaacacc gctggcatgg gcgcaattcc ggcgaatatt cactggggcg cacagacgct
    29341 aaccgcaggc gatgtattac tggttagcgg cacgctcggc gaccacgggg cgactatcct
    29401 taacctgcgt gagcagctgg ggctggatgg cgaactggtc agcgactgcg cggtactgac
    29461 gccgcttatt cagacgctgc gtgacattcc cggcgtgaaa gcgctgcgtg atgccacccg
    29521 tggtggtgtg aacgcggtag ttcatgagtt cgcggcagcc tgcggttgtg gtattgaaat
    29581 ttcagaagcg gcactgccgg ttaaacctgc cgtgcgcggc gtttgcgaac tgctgggact
    29641 ggacgccctt aactttgcca acgaaggcaa actggtgatc gccgttgaac gcaacgcggc
    29701 agagcaagtg ctggcagcgt tacattccca tccactggga aaagacgcgg cgctgattgg
    29761 tgaagtggtg gaacgtaaag gtgttcgtct tgccggtctg tatggcgtga aacgaaccct
    29821 cgatttacca cacgccgaac cgcttccgcg tatatgctaa taaaattcta aatctcctat
    29881 agttagtcaa tgaccttttg caccgctttg cggtgctttc ctggaagaac aaaatgtcat
    29941 atacaccgat gagtgatctc ggacagcaag ggttgttcga catcactcgg acactattgc
    30001 agcagcccga tctggcctcg ctgtgtgagg ctctttcgca actggtaaag cgttctgcgc
    30061 tcgccgacaa cgcggctatt gtgttgtggc aagcgcagac tcaacgtgcg tcttattacg
    30121 catcgcgtga aaaagacacc cccattaaat atgaagacga aactgttctg gcacacggtc
    30181 cggtacgcag cattttgtcg cgccctgata cgctgcattg cagttacgaa gaattttgtg
    30241 aaacctggcc gcagctggcc gcaggtgggc tatacccaaa atttggtcac tattgcctga
    30301 tgccactggc ggcggaaggg catatttttg gtggctgtga atttattcgt tatgacgatc
    30361 gcccctggag cgaaaaagag ttcaatcgtc tgcaaacatt tacgcagatc gtttctgtcg
    30421 tcaccgaaca aatccagagc cgcgtcgtca acaatgtcga ctatgagttg ttatgccggg
    30481 agcgcgataa cttccgcatc ctggttgcca ttaccaacgc ggtgctttcc cgcctggata
    30541 tggacgaact ggtcagcgaa gtcgccaaag aaatccatta ctatttcgac attgacgata
    30601 tcagtatcgt cttacgcagc caccgtaaaa acaaactcaa catctactcc actcactatc
    30661 ttgataaaca acatcccgcc cacgaacaga gcgaagtcga tgaagccgga accctcaccg
    30721 aacgcgtgtt caaaagtaaa gagatgctgc tgatcaatct ccacgagcgg gacgatttag
    30781 ccccctatga acgcatgttg ttcgacacct ggggcaacca gattcaaacc ttgtgcctgt
    30841 taccgctgat gtctggcgac accatgctgg gcgtgctgaa actggcgcaa tgcgaagaga
    30901 aagtgtttac cactaccaat ctgaatttgc tgcgccagat tgccgaacgt gtggcaatcg
    30961 ctgtcgataa cgccctcgcc tatcaggaaa tccatcgtct gaaagaacgg ctggttgatg
    31021 aaaacctcgc cctgaccgag cagctcaaca atgttgatag tgaatttggc gagattattg
    31081 gccgcagcga agccatgtac agcgtgctta aacaagttga aatggtggcg caaagtgaca
    31141 gtaccgtgct gatcctcggt gaaactggca cgggtaaaga gctgattgcc cgcgcgatcc
    31201 ataatctcag tgggcgtaat aatcgccgca tggtcaaaat gaactgcgcg gcgatgcctg
    31261 ccggattact ggaaagcgat ctgtttggtc atgagcgtgg ggcttttacc ggtgccagcg
    31321 cccagcgcat tggtcgtttt gaactggcgg ataaaagctc cctgttcctc gacgaagtgg
    31381 gcgatatgcc actggagtta cagccgaagt tgctgcgtgt attacaggaa caggagtttg
    31441 aacgtctcgg cagcaacaaa atcattcaga cggatgtgcg tctaatcgcc gcgactaacc
    31501 gcgatctgaa aaaaatggtc gccgaccgtg agttccgtag cgatctctat taccgcctga
    31561 acgtattccc gattcacctg ccgccactac gcgagcgtcc ggaagatatt ccgctgctgg
    31621 cgaaagcctt taccttcaaa attgcccgtc gtctggggcg caatatcgac agcattcctg
    31681 ccgagacgtt gcgcaccttg agcaatatgg agtggccggg taacgtacgc gaactggaaa
    31741 acgtcattga gcgcgcagta ttgctaacac gcggcaacgt gctgcagctg tcattgccag
    31801 atattgtttt accggaacct gaaacgccgc ctgccgcaac ggttgtcgcc caggagggcg
    31861 aagatgaata tcagttgatt gttcgcgtgc tgaaagaaac caacggcgtg gttgccgggc
    31921 ctaaaggcgc tgcgcaacgt ctggggctga aacgcacgac cttgctgtca cggatgaagc
    31981 ggcttggaat tgataaatcg gcattgattt aactgcaaat tgccggacag atctgcctgt
    32041 ccggcatact attcacgagt ttttttcgga cgatattttt ccggcagttc tggcaccgga
    32101 cgcttgtcat cgatgagatg acgcacggtt aagatcggat gacgccacag cattcgcggc
    32161 ccggcccaac gcataatctg tttcatctct tcacgctttg ctggctggta acagtgcacc
    32221 ggacactgct tacaggctgg tttctcttcg ccgaacacac atttatccag ccgcttttgc
    32281 gcgtaaacaa acaacgcctc gtaatgctcc ggctccgctg acgcctgcgg gcatttcgct
    32341 tgataaagat cgatcatttt tttaatcgtc agtttttcac gagagatacg cttgccggac
    32401 atgctgcctc catactctta aggtgtattt atattacaac ttaatttgaa aggggactat
    32461 ggctctgaag gaggaggttt attcaaccga cgcaattttg cgtacttgct tcataagcat
    32521 cacgcaaaag ctgcaaaaca gcatctttcc cggaaccagc atcaagaact cgccgttcgc
    32581 ttcttcccct gaaatgatta actccggtat catgtgcgcc ttatgtgatt acaacgaaaa
    32641 taaaaaccat cacaccccat ttaatatcag ggaaccggac ataaccccat gagtgcaata
    32701 gaaaatttcg acgcccatac gcccatgatg cagcagtatc tcaagctgaa agcccagcat
    32761 cccgagatcc tgctgtttta ccggatgggt gatttttatg aactgtttta tgacgacgca
    32821 aaacgcgcgt cgcaactgct ggatatttca ctgaccaaac gcggtgcttc ggcgggagag
    32881 ccgatcccga tggcggggat tccctaccat gcggtggaaa actacctcgc caaactggtg
    32941 aatcagggcg agtccgttgc catctgcgaa caaattggcg atccggcgac cagcaaaggt
    33001 ccggttgagc gcaaagttgt gcgtatcgtt acgccaggca ccatcagcga tgaagccctg
    33061 ttacaggagc gtcaggacaa cctgctggcg gctatctggc aggacagcaa aggtttcggc
    33121 tacgcgacgc tggatatcag ctccgggcgt tttcgcctga gcgaaccggc cgaccgcgaa
    33181 acgatggcgg cagagctgca acgcactaat ccggcggaac tgctgtatgc agaagatttc
    33241 gccgagatgt cgctgattga aggccgtcgc ggcctgcgcc gtcgcccgct gtgggagttt
    33301 gaaatcgaca ccgcgcgcca gcagttgaat ctgcaatttg gcacccgcga tctggtcggt
    33361 tttggcgtcg agaacgcgcc gcgcggactt tgtgctgccg gttgtctgtt gcagtatgcg
    33421 aaagataccc aacgcacgac cctgccgcat attcgttcta tcaccatgga acgtgagcag
    33481 gacagcatca ttatggatgc cgcgacacgt cgtaacctgg aaattactca gaacctggcg
    33541 ggcggtgcgg aaaatacgct ggcttctgtg ctcgactgca ccgtcacgcc gatgggtagt
    33601 cgtatgctga aacgctggct gcatatgcca gtgcgcgata cccgcgtgtt gcttgagcgc
    33661 cagcaaacta ttggcgcatt gcaggatttc accgccgagt tgcagccggt actgcgtcag
    33721 gtcggcgacc tggaacgtat tctggcacgt ctggctttac gaactgctcg cccacgcgat
    33781 ctggcccgta tgcgccacgc tttccagcaa ctgccggagc tgcgtgcgca gttagaaact
    33841 gtcgatagtg caccggtaca ggcgctacgt gagaagatgg gcgagtttgc cgagctgcgc
    33901 gatctgctgg agcgagcaat catcgacaca ccgccggtgc tggtacgcga cggtggtgtt
    33961 atcgcatcgg gctataacga agagctggat gagtggcgcg cgctggctga cggcgcgacc
    34021 gattatctgg agcgtctgga agtccgcgag cgtgaacgta ccggcctgga cacgctgaaa
    34081 gttggcttta atgcggtgca cggctactac attcaaatca gccgtgggca aagccatctg
    34141 gcacccatca actacatgcg tcgccagacg ctgaaaaacg ccgagcgcta catcattcca
    34201 gagctaaaag agtacgaaga taaagttctc acctcaaaag gcaaagcact ggcactggaa
    34261 aaacagcttt atgaagagct gttcgacctg ctgttgccgc atctggaagc gttgcaacag
    34321 agcgcgagcg cgctggcgga actcgacgtg ctggttaacc tggcggaacg ggcctatacc
    34381 ctgaactaca cctgcccgac cttcattgat aaaccgggca ttcgcattac cgaaggtcgc
    34441 catccggtag ttgaacaagt actgaatgag ccatttatcg ctaacccgct gaatctgtcg
    34501 ccgcagcgcc gtatgttgat tattaccggc ccgaacatgg gcggtaaaag tacctatatg
    34561 cgccagaccg cactgattgc gctgatggcc tacatcggca gctatgtacc ggcacaaaaa
    34621 gtcgagattg gacctatcga tcgcatcttt acccgcgtag gcgcggcaga tgacctggct
    34681 tccgggcgct caacctttat ggtggagatg actgaaaccg ccaatatttt acataacgcc
    34741 accgaataca gtctggtgtt aatggatgag atcgggcgtg gaacgtccac ctacgatggt
    34801 ctgtcgctgg cgtgggcgtg cgcagaaaat ctggcgaata agattaaggc attgacgctg
    34861 tttgccaccc actatttcga gctgacccag ctaccggaga aaatggaagg cgtcgccaac
    34921 gtacatctcg atgcgctgga gcacggcgac actatcgcct ttatgcacag cgtgcaggac
    34981 ggcgcggcga gcaaaagcta cggtctggcg gttgctgctc tggcgggcgt accgaaagag
    35041 gttattaagc gcgcacggca aaaactgcgt gagctggaaa gcatttcgcc gaacgccgcc
    35101 gctacgcaag tggatggtac gcaaatgtct ttgctgtccg taccagaaga aacttcgcct
    35161 gcggtcgagg cactggaaaa cctggacccg gattcactga ctccgcgtca ggcgctggag
    35221 tggatttatc gcttgaagag tctggtgtaa taacaactcc cgatagtctt ttgctatcgg
    35281 gaatattaac gacaactgac gaatcaaata aaaataccct gtataatagg aaagcttatt
    35341 ttacagggtc aaaccatgcc atctacacgc tatcaaaaaa tcaatgccca tcactatcgc
    35401 catatatggg tcgttggtga tattcatggt gaatatcagt tattacaatc ccgcttacat
    35461 caactctctt ttttccccga aaccgactta cttatttcgg taggcgataa catcgatcgt
    35521 gggccggaga gtcttgacgt cctgcgcctg ctaaaccagc cctggtttat ctccgttaaa
    35581 ggcaaccacg aagcaatggc gctggatgcg ttcgcgaccg gcgatggcaa tatgtggctt
    35641 gccagcggtg gtgactggtt tttcgattta aatgattcag agcaacaaga ggcaatagat
    35701 ctgttgctga aattccatca ccttccacat attattgaaa tcactaacga caacataaaa
    35761 tatgtcatcg cacatgcaga ttatccgggg agtgaatatc tctttggtaa agaaatagcg
    35821 gagagcgaat tactctggcc tgttgatcgt gtgcagaaat cgcttaatgg cgagttacaa
    35881 caaataaacg gcgctgatta ttttatattt ggacatatga tgtttgataa cattcagacg
    35941 ttcgctaacc agatttatat tgataccggc acgccgaaaa gcgggcggct gtcattttat
    36001 aaaataaagt agtctcatgc ttcttctgtg aagcatgagt aaccaaatgt tattgcaggc
    36061 cattattcat ttttcgcaac cagcaaagag agatcctgct tcgccagcgc gcgactggca
    36121 ctctccggca aaccgtcgtc tgtgataatc tgatcaaact cgcttaatgg taacgccagc
    36181 catgtcgcca cctgaccata tttcgtcgca tcacagacca aaactcgctg gcggctggca
    36241 ctggcaatcg cccgtttcac cgtgacttta tcttccgctg gcgtagaaat cccccgcaca
    36301 ctccatgacg atgcagaaat aaaagcctga tcaatcatca ggctgcgcag catggtcgca
    36361 gcggcttccc cgacacagga acggttttcc cgacacactg caccgccagt gtgaataatt
    36421 gtgcaattac tgttgtcgag caagtagttc gcaataacga aatcgtttgt gaccacagtc
    36481 agtgactcca tgtgaatcag atgctgtgct atcgctaacg tggtcgttcc cgcatccaga
    36541 tagatacaac ttcccggctg aacaagactt gccgccagct tgccaatagc cgctttttgc
    36601 gtcattgcca gcgcagtttt tacctgatga gaaggttcat gcgccacgcg tcccggagac
    36661 tggacgcctc cggacaccag cacaacggct ccctgctgct ccagtttttg taaatcccga
    36721 cgaatggtca tatgtgacac attcattctg tccgttagtt cagcaatact gacaatgcct
    36781 ttttcagcta ccatctcaag gatgatttgg cgacgctcta cgggtatcaa cttttgctcc
    36841 ttcctttgtc ctgctgacat tctacgctat ttgcctgcga aacgtgcgcg gcgcaactaa
    36901 cgcttagttc acataaaata acacacaatg ttaatttatg tgaatcagct caccatatcg
    36961 ttatcttcca gcgcttatat tcacaatatc aaacaaaata tcacttaaat taacaaggag
    37021 agcagatgaa aacgggatct gagtttcatg tcggtatcgt tggcttaggg tcaatgggaa
    37081 tgggagcagc actgtcatgt gtccgcgcag gtctttctac ctggggcgca gacctgaaca
    37141 gcaatgcctg cgctacgttg aaagaggcag gtgcttgcgg ggtttctgat aacgccgcga
    37201 cgtttgccga aaaactggac gctctgctgg tgctggtggt caatgcggcc caggttaaac
    37261 aggtgctgtt tggtgaaaca ggcgttgcac aacatctgaa acccggtacg gcagtaatgg
    37321 tttcttccac tatcgctagt gctgatgcgc aagaaattgc taccgctctg gctggattcg
    37381 atctggaaat gctggatgcg ccagtttctg gtggtgcagt aaaagccgct aacggtgaaa
    37441 tgactgtcat ggcctccggt agcgatattg cctttgaacg actggcaccc gtgctggaag
    37501 ccgttgccgg aaaagtttat cgcataggtg cagaaccggg actaggttcg accgtaaaaa
    37561 ttattcacca gttgttagcg ggcgtacata ttgctgccgg agccgaagcg atggcacttg
    37621 cagcccgtgc ggggatcccg ctggatgtga tgtatgacgt cgtgaccaat gccgccggaa
    37681 attcctggat gttcgaaaac cggatgcgtc atgtggtgga tggcgattac accccgcatt
    37741 cagccgtcga tatttttgtt aaggatcttg gtctggttgc cgatacagcc aaagccctgc
    37801 acttcccgct gccattggcc tcaacagcat tgaatatgtt caccagcgcc agtaacgcag
    37861 gttacgggaa agaagacgat agcgcagtta tcaagatttt ctctggcatc actctaccgg
    37921 gagcgaaatc atgatcaaga ttggcgttat cgccgatgat tttaccggcg cgacggatat
    37981 cgccagtttt ctggtggaaa acggtctacc aacggtacaa attaacggtg ttccaacagg
    38041 taaaatgccg gaagcaatcg acgcactggt gatcagcctg aaaacgcgct cctgtccggt
    38101 ggttgaagcc acacagcaat cgctggcggc tctgagctgg ttgcaacagc aaggttgcaa
    38161 acagatctat ttcaaatact gctctacttt cgacagtacg gcgaaaggta atatcggccc
    38221 ggttaccgat gcattaatgg atgctctcga cacgccgttt acggtcttct ctccggccct
    38281 gccggtcaac ggacgtacgg tttatcaggg gtatttgttc gtaatgaatc aactgctggc
    38341 cgaatccggg atgcgccatc acccggtaaa tcccatgacc gacagctatc ttccccgtct
    38401 ggttgaagcg caatccacag ggcgctgcgg cgtcgtttcg gcacatgttt tcgaacaagg
    38461 tgtggatgcc gttcgtcaag agctggctcg cttacagcaa gagggctacc gctacgcggt
    38521 gcttgatgcg ctgaccgaac accatctgga aattcaggga gaagccttgc gcgatgcccc
    38581 actggtaacg ggcggttctg gtctggcgat tggcctggcc cggcagtggg cgcaagaaaa
    38641 cggtaaccag gctcgcgaag cagggcatcc gctcgctggg cgcggcgtag tgctctccgg
    38701 ttcatgctct caaatgacca accgccaggt ggcacattac cgtcaaattg caccagcccg
    38761 tgaagttgat ctggcacgct gcctctcaac cgaaactctg gccgcttatg cacacgaact
    38821 ggcagagtgg gttctgggcc aggaaagtgt acttgctcca ctggtttttg ccaccgccag
    38881 cactgacgca ttggcagcaa ttcaacagca atacggtgca caaaaagcca gtcaggcagt
    38941 agaaactcta ttttctcaac tagcggcgcg gttagcagcg gaaggcgtga cacgctttat
    39001 tgtcgcaggc ggtgagacct ccggcgtagt cacacagagc ctgggcataa aagggtttca
    39061 tattggccca accatttccc ccggcgtgcc gtgggtaaac gcactggata agcctgtctc
    39121 actcgccctt aaatctggca acttcggtga tgaagccttt ttttcacgag cccaaagaga
    39181 gtttttatca tgagcgattt cgcaaaagta gagcagtctt tgcgagagga gatgacgcgg
    39241 attgccagtt cattctttca gcgcggctat gcaaccggtt cggctggcaa tctgtcgctg
    39301 cttttacctg acgggaattt actggcgaca ccaacaggtt catgcctggg caatctcgat
    39361 ccgcagcggc tttccaaagt cgccgcggat ggcgaatggt taagtggtga caaaccctcg
    39421 aaagaggtgc tctttcatct ggcgctgtat cgcaacaatc cgcgctgtaa agcggtggtg
    39481 catttgcaca gcacatggtc gacggcgctt tcctgcctgc aagggctgga cagcagcaac
    39541 gttattcgtc cgttcacacc atacgtggtg atgcggatgg gaaatgtccc gctggtgcct
    39601 tattaccgac cgggcgataa acgcatcgca caggatctgg cggaactggc agcagacaat
    39661 caggcttttt tactggcaaa tcatggccca gtggtttgcg gtgaaagcct gcaagaagcc
    39721 gccaacaata tggaagagct ggaggaaacg gcaaagctga tttttattct cggtgaccgc
    39781 ccgatccgtt atctgaccgc aggtgaaatt gcggaattaa ggagttaatg caatgcctcg
    39841 ttttgcagct aatttatcca tgatgttcac cgaagtgcct tttattgaac gcttcgccgc
    39901 agcgcgaaaa gccgggttcg atgctgtgga atttctgttt ccctatgact actccaccct
    39961 gcaaatccaa aagcaactgg agcaaaacca tctgacactg gcgctgttca ataccgctcc
    40021 cggagatatt aatgcggggg aatggggatt atccgccctt cccggacgtg agcacgaagc
    40081 acacgcagac atcgacctgg cactggaata tgcattggcg ctcaattgtg aacaagtcca
    40141 tgtgatggca ggcgtcgtgc ccgctggcga agacgctgaa cggtaccggg cagtatttat
    40201 cgataatctc cgctacgctg ccgaccgctt tgcgctacac ggcaagcgaa tattagttga
    40261 agcactcagc cccgacgtta agcctcatta tctcttctcc agccagtatc aggcactggc
    40321 tattgttgaa gaggttgcgc gagataacgt gtttattcaa ctagatactt ttcatgcgca
    40381 aaaagtagat ggcaacctga cgcatttaat ccgcgactac gctgggaaat atgcgcacgt
    40441 acaaattgcc ggactacctg atcgacatga accggacgat ggagaaatca actatccgtg
    40501 gctgttccgc ctgttcgatg aggtgggata tcagggctgg atcggttgtg aatataaacc
    40561 tcgtggcctc accgaagaag ggcttggctg gtttgacgcc tggcgctaat tcgttaaatc
    40621 cctaattaca acgtacccat acatcccccc atctacggat ggggtaggtt aactataact
    40681 ttcagacagg gtttcccatg tccacaatta cattgttatg cattgcgtta gctggcgtaa
    40741 tcatgctgtt gctgctggtc atcaaggcaa aggtacaacc attcgttgct ctgctcctcg
    40801 tcagcctgtt agtcgcactt gcggcgggta taccggcggg cgaagtgggt aaagtgatga
    40861 tcgccgggat gggcggtgtg cttggttccg tcactattat tattggtctg ggcgctatgc
    40921 tggggcgtat gatcgaacac tctggtggtg cagagtcact ggctaattat ttcagtcgca
    40981 agttaggtga caaacgaact atcgctgcgc tgactctggc agcgttcttc ctcggtattc
    41041 ccgtcttctt tgatgtcggc tttattattc ttgcgccaat catttacggt tttgccaagg
    41101 ttgccaaaat atcgccactc aaatttggcc tgcctgtcgc tggaatcatg ctaactgttc
    41161 atgtggcggt accgccgcat ccaggccctg tcgccgcagc ggggttactc cacgcagaca
    41221 tcggctggct aaccatcatc ggtattgcga tttctattcc cgtagggatt gttggctact
    41281 ttgcagcgaa aataatcaat acgcgccaat atgcgatgtc ggtggaagta ctggaacaga
    41341 tgcaactggc tccggccagt gaggaaggcg caacaaaatt aagcgataaa ataaatccac
    41401 cgggcgtcgc gctggtcacc tcgctaattg ttattcctat cgcgattatc atggcgggta
    41461 cggtttccgc aacactgatg ccgccttcgc atcccctgct tggtacgcta cagctgatcg
    41521 gctcaccaat ggtagctcta atgattgcgc tggtgctggc attctggtta ttggctttac
    41581 gtcgcggctg gagcttacaa cacaccagcg acattatggg ctcagccctt cccactgcgg
    41641 cggtagtgat tttggttact ggtgctggag gggtatttgg caaagtgctg gtggaatcgg
    41701 gcgtcggcaa agcccttgcc aatatgctac aaatgattga cctgcctctg ttaccagccg
    41761 catttattat ttcactggct ctgcgtgcat cgcaggggtc agcaaccgta gcaatcctga
    41821 caaccggcgg gttactctca gaagcggtga tgggattgaa tccgattcag tgcgtattgg
    41881 tgacgctggc agcctgcttt ggtgggcttg gcgcgtcaca tattaatgac tcagggttct
    41941 ggattgtgac caaatatctg gggttgtcgg tagcagacgg tctgaaaacc tggacagtgt
    42001 taacgaccat tctcggtttt accggattct taattacctg gtgcgtatgg gcggtaattt
    42061 gaccttaata aaaaggtccg atggacatcg gaccttttat ttaacgctta ccttccacca
    42121 gcagcggcgg gatgctcggc accattggcg cgtcatcaat atctttctgc gtcatgcgga
    42181 acgcttcggg ataatgttcg cggctggtac ggcgcagcgg ttcggtatcg cgccaggtat
    42241 aaaggcaatg ctggcactga tataccgtcc agacatcttt caccggcgat ttcgccatca
    42301 cttcaatctg ttcatcggca caacgtggac aaatcatctt cttctcctta tttacgtgca
    42361 gccagcatag cggtcagttt ttcagcccag gctttggttt ccggtaaatc caccaccggt
    42421 tggctgtagt gaccacggtt gtccggggcg acaggcgtag tggcgtcgat aatcagcttg
    42481 tcggtgatcc ccgcagggct tgagcctgga tcgagttcca gcacggacat attcggcaac
    42541 tgcaccaaat cccctgccgg gttcactttc gaggagagcg cccacatcac ctgcggcagg
    42601 ttgaacgggt caacgtcttc atcgaccata atcaccatct tcacgtagcc cagaccatgc
    42661 ggcgtggtca ttgcgcgcag gcccaccgcg cgggcaaagc cgccgtagcg ttttttggtg
    42721 gagataatcg ccagcaggcc atgggtgtac atggcgttta ccgcctgcac ttccgggaac
    42781 tcggctttca gctgctgata cagcggcacg caggtggctg gccccatcag gtagtcgatt
    42841 tcggtccacg gcataccgag gtacagcgat tcgaaaatcg gcctggtgcg gtaagagact
    42901 ttatcgatgc gcaccacggt catgttacgc ccgccggagt agtgcccggt aaactcaccg
    42961 aacggccctt cgatttcgcg tttacggctt tcgatgaccc cttcgaggat cacttctgaa
    43021 ccccacggca catcaaaacc ggtcaacggg gcggtggcga tcgggtacgg gctttcacgc
    43081 agcgcgcctg ccatttcgta ctcggactga tcatatttca gcggcgtggc ccccatcagc
    43141 gtgatgatcg gatcgttacc gagcgtgatc gcaatcggca gatcttcacc gcgctcttct
    43201 gctttatgca gatgcagggc gatatcgtgc atcggcaccg gttgcaggcc gagcttacgc
    43261 ttgcccttca cttccatgcg gtagatgccg acgttctgct tgccgaagtt atccgggtcg
    43321 agcggatcgc gggaaaccac gcacgctttg tcgagataga aaccgccatc gccatcgttt
    43381 aaacgaaaca gcggcaggat atcgaacagg ttgatctcgt cgccatcaac ggtgttctgc
    43441 gcccaggctg gattggcgcg gcgctccggg gcaatcggga agttatccca gcggcggata
    43501 aactcatcaa tctgcttttt aaccggggca tttggcggca ggccgaggga aatcgcgtgg
    43561 ttctgccagg aaccgatggt gttcatcgcc acgcgggcat cggtaaagcc gcgaatatta
    43621 tcaaaccaca gcgcgggcgc gccgtcgccg atacgcccgg tggcgttagc tgctgctgcc
    43681 agatccggct cggcgttcac ttcttcgctg attttcagta actggccgtg gtcatcaagc
    43741 tcctgtaaaa agctgcgtaa atcatcaaat gccattattc attctcctga gaaaaattcc
    43801 gggcctgcgg caatccttgc cagcgcctgg cgtggggatg ttcgaggcca aattgatcca
    43861 gcacgcgggc taccacatgg tggacaatgt catctaccgt ttcgggatgg ttataaaagg
    43921 caggcatcgg cggcaccatc gccacgccca tgcgtgaaag tgcgagcata ttttcgagat
    43981 ggatggtgct aagcggcatt tcacgcggca ccagcaccag tttgcggcct tctttgagca
    44041 cgacgtccgc cgcgcgccct accaggccat cggcgtaacc agcgcggata ccggcgagcg
    44101 ttttcatact gcacggaata acgatcatgc cgtcggtacg aaaagaaccg gatgagatga
    44161 tcgccgcctg atccgccggg ttatggctga agtctgcgag ggcagcaaca tcgcgagcgc
    44221 tgtaaggcgt ttccagttca atggtggttt tcgcccactt cgacatcacc agatgagtct
    44281 cgacattcgg catctcccgc agcgcttgca gtaatgccac accaagaggc gcaccggtag
    44341 cccctgtcat cccgacgatc agtttcattg cttattctct taattgttcg tatacgaacg
    44401 ttattattaa catactcctt catatccaca gcggcaagga ttgaaaagta acaacatctt
    44461 cttcgtcact aaaaaaccgc tatgatagcg acagagaatt agcccggagt ttacatggcg
    44521 ttacgaaata aagcgttcca tcagttacga cagctttttc agcagcacac ggctcgctgg
    44581 cagcacgagt tacctgacct gaccaaacca cagtacgcgg tgatgcgcgc cattgctgat
    44641 aaacctggta tcgaacaggt ggcactgata gaagcagcag tcagcaccaa agcaacgcta
    44701 gcagaaatgt tggcaagaat ggaaaatcgt ggcttagtca gacgtgaaca tgactccgct
    44761 gacaagcggc gacgctttgt ctggctgact gccgaaggag aaaaggtact tgctgcggcg
    44821 atacctattg gtgacagcgt ggatgaggaa tttttggggc gtttgagtgg agaagagcag
    44881 gagctgttta tgcagctggt gcgcaagatg atgaacgcat agggggcaaa tttccggatg
    44941 cgacgctatc gcgtcttatc cagactacaa atacaccgac tcaccaaccg aaagaaaaaa
    45001 ggccagcctc gcttgagact ggcctttctg acagatgctt acttactcgc ggaacagcgc
    45061 ttcgatattc agcccctgcg tttgtaggat ttcgcgcaaa cggcgcaggc cttcaacctg
    45121 aatctggcga acacgttcac gggtgaggcc aatttcacga cctacatctt ccagtgttgc
    45181 cgcttcgtac cccagcaaac cgaatcgacg tgccagcact tcacgctgtt tggcgttcag
    45241 ctcgaacagc catttgacga tgctctgctt catatcatca tcttgcgtgg tatcttccgg
    45301 accgttctct ttttcatcgg ccaggatgtc cagcaacgct ttttcggaat caccacccag
    45361 cggggtgtct accgaggtaa tgcgctcgtt aagacgaagc atacggctga cgtcatcaac
    45421 tggcttatcc agttgctctg cgatctcttc cgcacttggt tcatggtcca gcttatggga
    45481 caactcacgt gcggttcgca ggtaaacgtt cagctccttt acgatgtgaa tcggcaaacg
    45541 aatagtacgg gtttggttca taatcgcccg ttcaatcgtc tggcgaatcc accaggttgc
    45601 gtatgtcgag aagcggaaac cacgttccgg gtcaaacttc tctaccgcgc ggatcagccc
    45661 caggttgccc tcttcgataa ggtccagcaa cgccagacca cgattgccat aacggcgggc
    45721 aatttttacc accagacgca agttactctc gatcatccgg cggcgagagg cgacatctcc
    45781 acgcagtgcg cgacgcgcaa aataaacttc ttcttcggcc gttaacagtg gtgaataacc
    45841 aatctcacca aggtaaagct gagtcgcgtc caacacacgc tgtgtggctc cctgcgataa
    45901 cagttcctct tcggccaaat cgttatcact gggttcctct tctactaagg ccttttcgtc
    45961 aaaaacctca actccgttct catcaaattc cgcatcttca tttaaatcat gaactttcag
    46021 cgtattctga ctcataaggt ggctcctacc cgtgatccct tgacggaaca ttcaagcaaa
    46081 agcctggttc cgccgattta tcgctgcggc aaataacgca gcgggtttac ggatttcccc
    46141 ttgtaacgaa tttcaaaatg caagcgtgtt gaactggttc cggtgctacc catggttgct
    46201 attttttgcc ccgccttcac ttcttgttgt tcccggacca gcattgtgtc gttatgggcg
    46261 taggcactca ggtaatcatc attatgtttg atgataatca gattaccgta gccgcgcagc
    46321 gcgttaccgg cataaacaac gcggccatct gcggtcgcga taattgcctg tcctttgctg
    46381 cctgcgatat caatcccctt gttgcccccc tcagaagcgc caaaggtttc gatcactttg
    46441 ccctcagtcg gccagcgcca ggtggagata ggcgtactgg ttgatgtact gctgacagtc
    46501 ggctcggttg tgcttgctgt tggtaccgtt acaggcgctg tgaccgtggt cgcagttggc
    46561 ttgttgttcg gcaacatttt gttagcactc tgttcacccg aagactcaga atacgtaatt
    46621 gtcggttgcg acgcaacagc aacggtggaa ttttgtgcag gcttgatcac aactccttgc
    46681 tctgctgcat cggcctgggt aatggcattt ccgccagtga ttggcgtacc ggaagcatta
    46741 cccacctgta aggtctgacc gacgttcagc gcgtatggtg cctgaatatt gttgcgctga
    46801 gcaaggtcac ggaaatcgtt gccagtaatc caggcgatat agaaaagtgt gtcgcctttt
    46861 ttcacggtat aggtactgcc gctataactg cctttcggaa tgttcccata ctgacggtta
    46921 tagacgatgc gtccgttttc catctgtacc ggctgctgag ctactggctg tactggctgg
    46981 atttgcggtt gttgagtagc ctgaatttgt ggctgctgta ccggctgaat ttgcggttgc
    47041 tgcgctgtag acgtcgttcc cattttcggc ggcggcgtaa tcaacatacc agaattggta
    47101 tttgcaggcg cattgccatt aacggagctg accggtgccg gtggatttga agtgtcagaa
    47161 cagcctgcca gccatagcga aaccagtgac aaagccgcaa tgcggcgaac ggtgaatttt
    47221 gggcttcccg cgctcattta tcccccagga aaaattggtt aataaccagt gacataatta
    47281 ccgtgcaagg caccatactg aacactggaa aagatgttca cgatacgctg acctgcggca
    47341 aaataaccag gaaaaatcca ggtatttcct cacgttttaa gccagctcac ccttcactaa
    47401 agggacaaag cgcacggcct ccacggtatc gataataaat tcgcctcccc gacgacgcac
    47461 ccgtttcaaa tactggtgct cctcccctac gggtaagacg agaatcccgc cttcgtccag
    47521 ctgcgtcatt agcgcagttg gaatttccgg cggtgccgcc gtaacaatga tagcgtcaaa
    47581 cggcgcacgt gcctgccaac cttgccatcc atcgccatga cgggttgaaa cattatgtaa
    47641 atcaagattt ttcaggcggc gacgcgcctg ccactgcaag cctttaatcc gttcaaccga
    47701 gcaaacatgc tggacaagat gcgccaggat tgccgtttga tatcccgaac cagtgccaat
    47761 ttccagcacc cgcgactgcg gcgtcagctc gagtaattcg gtcattcgcg ccaccatata
    47821 cggctgcgaa attgtctgcc cctgacctat cggcaaggcg atattgtccc aggctttttg
    47881 ttcaaacgct tcatcaacga atttttcacg cggcacggcg gcaagtgcat tcagcacctg
    47941 ctcatcctga ataccttgcg cacgtaattg atccagaagt gcttgtacgc gtctgcttac
    48001 cattgcgtgc caactcccac gctgtttaac cagtctgaaa ccacatcttg cgcgctatgc
    48061 gcagttaaat ccacatgcag cggcgtgatg gagacatagc cttcatctac cgctgcgaag
    48121 tcggtatccg gcccggcgtc acatttcccc cccggaggac caatccagta cagtgtatta
    48181 ccacgaggat cttgctgcgg gatcacctga tctgccggat gacggctccc acagcgagtc
    48241 acgcggattc ctttgatttg gtccagcggc aaatccggta catttatatt aagaatacgc
    48301 cctgttcgca gcggttcttt gcacagtgca cgcagaatag aacaggtaac tgccgcagcg
    48361 gtgtcgtaat gtttatgccc gtctagcgag acggcaagcg ccggaaaacc taaatggcga
    48421 ccttccatcg cggcggctac cgtaccggaa taaataacat catcccccag attcggcccg
    48481 gcgttaatcc cggacacaac aatgtccggg cgcggacgca tcagagcatt cacgccaaga
    48541 tagacgcaat cggtcggggt tcccatttgc acagcaatat caccattttc aaaggtaaac
    48601 gtgcgcaggg aggattccag tgtcagagaa tttgaagcgc cgctgcggtt acgatcgggg
    48661 gcgaccacct gaacgtcagc aaactcacgc aaggttttcg ccagcgtttg tataccgggt
    48721 gcatgtaccc cgtcatcatt actcagcaat atgcgcataa tcacctgttg tgttgataag
    48781 ttccctgaca acgctggttg caaaactacc cgccggaagc cagaaacgga tctctacggt
    48841 gacgtcatcc caccaattcc agcttaattg ttgcggatac agcagcatcg ctctgcgcgc
    48901 ggcttcaact ttttcgcgca ccagtaaagc ttgtaattca gtttctgcgg cgacagctgc
    48961 ttgttcgaat gccagcgctt cacgctgagt tccccattcg ccactgcccg gcaatgcggc
    49021 ggttatcatc aactctttat cgttgacgcg acgctgtaat tccgccagtt cttcggtggt
    49081 tgctacaaac cagctaccac gtccggctaa ttgtagcgca tcgccgtcaa caacttgatt
    49141 aacgtctgct tttttgaggc gctcagcaac aatctgatta aacaacgcac tgcgggctgc
    49201 cgacaaccaa aaactccgtt tattacgatc gcgcaccgga gtattggttt gcgcccagcg
    49261 cagcgcgccc tgcaagttgc taccgccaat cccaaaacgt tgggcaccga agtagttcgg
    49321 tacacctttt acgcaaatat cgatcagacg ttgttcaacg tcatcgcgat tgctcacttc
    49381 gcgcagaacc agggtaaagg cgttaccttt cagcgcgcct aaacgcagct tacgcttgtg
    49441 ccgcgcatac tccagcacct ggcagccttc cagttgaaag gcgctcagat cgggcatttc
    49501 cttgcctggc acgcgagcgc ataaccactg ttccgtaaca gcatgtttgt ctttttgccc
    49561 agcgaagctg acttcacggg catgaatttt caggaatttc gccagtgcat ccgccacaaa
    49621 acgggtattg cagccgtttt tgaggattct aaccagaata tgctcacctt caccatcagg
    49681 ctcaaagccc aaatcttcca ccaccacaaa gtcttccgga ttggctttca gcagcccggt
    49741 gccttgcggt ttaccgtgga ggtaagtgag attatcaaac tcaatcattt tgttgcctta
    49801 atgagtagcg ccaccgcttc acaggcaatc ccttccccac gtccggtaaa tccaagtttt
    49861 tccgtagtag tggctttcac gttaacatca tccatatggc agccgagatc ttcggcaata
    49921 aacacgcgca tttgtggaat gtgcggcaac atcttcggtg cctgagcgat gatagtgaca
    49981 tcgacgttgc caagggtata acccttcgcc tgaatacgac gccaggcttc gcgtagcagc
    50041 tcgcggctat cggcaccttt aaatgccgga tcggtatccg ggaacagctt gccgatatcc
    50101 cccagcgccg ccgcgccaag caatgcatcg gtcaacgcat ggagcgccac gtcgccatca
    50161 gaatgcgcca gcaatccttt ttcgtaagga atgcgtacgc caccaatgat aattgggcct
    50221 tcaccgccaa aggcgtgtac gtcaaaaccg tgtccaattc gcattatgta ttctcctgat
    50281 ggatggttcg ggtgaggtaa aactcggcca gtgccaaatc ttccgggcgc gtgactttaa
    50341 tgttatccgc acggccttcg accaactgag gatggaatcc gcaatattcc agcgccgagg
    50401 cttcgtcggt aatagtcgcg ccttcattta gagcgcgcgt cagacagtca tgtaacagct
    50461 cacgagggaa aaattgcggc gtcagcgcgt gccataagcc gttgcgatca acggtatgag
    50521 caatggcatt tttgcccggt tcggcacgtt tcatcgtatc gcgcactggt gcggctagga
    50581 tccctcccgt gcggctggtt tcgctcaacg ccaacaatcg cgcgaggtca tcctgatgca
    50641 gacaaggacg agcggcgtca tgcaccaata cccactgcgc gtcgccagcg gctttcaaac
    50701 ctgccagcac ggaatcggca cgctcatcac cgccatctac aacggtgatt tgcggatgat
    50761 tcgccagagg aagttgtgca aaacggctat cgccaggact tatggcaatg acgacacgtt
    50821 tcacccgggg atgcgccagc agcgcatgca ccgagtgttc aagaatggtt tgattaccga
    50881 ttgagagata ttgcttagga cattccgttt gcattcgacg gccaaatccg gccgccggaa
    50941 ccacggcgca aacatccaaa tgagtggttg ccatgttaat tcccgggctg atttatcgat
    51001 tgttttgccc cgcagactgt gcgcgcttcg acgcgtcagg caccagacga taaaaagttt
    51061 cgcccggcct ggtcatgctg agttcattac gcgcacgctc ttcgagcgcc tcctggccgc
    51121 cattgagatc gtcaatttcg gcaaaaagtt gatcgtttcg cgctttaagt ttcgcgtttg
    51181 tagcttgctg tgccgccaca tcatcattga cgcgggtata gtcatgtata ccgttcttac
    51241 cgaaccacag cgaatactgt agccagacca gaatagccag caacagcagc gttagtttac
    51301 ccatcctgcc ccctgaaaaa cggcatcatc atcccatgca tccgaagacg actctacatc
    51361 ctctgttggg gataccgcga caacgcgggc aaatgtacca catttgtcca ttgttacgta
    51421 tacccagggc gtgcagaaca taatctcatt attagttacg gtttgaatta tgaacagagg
    51481 agacaagaaa gtacaaatta gcccagtagc cacataaaca gtgcgccaaa cataatgcct
    51541 actgtcacca gggtgaaaac aatactgtag cgtagctttc cgtccatcaa tgaatgcagc
    51601 gcaatcccca ccactaccgc gacgggcatc agcgccagaa agaaaggcca ggtgtagata
    51661 aagaagaaca gcgtgttaga gccataaatc aacatcggca tcgccagcgc aaataaccag
    51721 gagataaaac cgaccacggc accaggcagt gaccatgtgg tttcttcatc ctcagtaagg
    51781 ctgtcgttat ttgttagtgt aatgttatgg ctattacgca tatttgatcc tgttactttg
    51841 acgaaccggg catggaaacc cggtggtgtc tcaggatctg ataatatcgt tctgtctcaa
    51901 cagatctaat aattgctgta ccaaatttgt tactaattgt tcaccattga gatgaatttc
    51961 tgccgattca ggcgcttcgt aaacggaatc tattcccgta aagttgcgca gttcaccggc
    52021 acgcgctttc ttatataagc ctttgggatc gcgggcttcg caaatcgcca gcggcgtatc
    52081 gacaaacact tcgataaagc gcccttctcc tacgcgttcg cgaaccatct ggcgttcggc
    52141 gcggtgtggc gagataaatg cggtcagcac taccagtccg gcttcaacca tcaaattcgc
    52201 cacttcaccg acgcgacgga tattctcttt acgatcggca tcgctaaaac cgagatcgct
    52261 gcataatccg tggcgaacat tatcgccatc cagcagatac gtactgacgc cgagtttatg
    52321 taacgcctcc tccagcgccc cggcgaccgt tgatttaccg gacccggaga ggccggtaaa
    52381 ccacagcact acaccacgat gaccgtggtg tagctcgcgt tgttgcacag tgaccggatg
    52441 gctatgccag acgacgtttt cgtcatgcag cgccattatt tatcccccag caaatcgcgc
    52501 gcgccccagt gcggaaagtg gcgacgaacc agagcattca attccagttc gaatgcactg
    52561 aattcagatg gcgcagcagt tgcctggcta actggctcgt gcaccatacc ggcacccacg
    52621 gtcacattgc tcaggcgatc gataaaaatc agcccacccg tcaccggatt ttgttgataa
    52681 cgatctaaca ccagcggctc gtcaaaagtg agatccacga ggccgatccc attcagtggc
    52741 aggttttcaa cttcacgctg ggtaaggtta ttaatatcaa cctgatagcg aatgccatca
    52801 acacgcgcgc gcgtcttctt accggcaatt ttgatgtcgt aactctgccc tggagaaagc
    52861 ggctgttccg ccatccatac cacatccacc gacgcgctct gcaccgccgg taacgcttcg
    52921 tctgccgcca gcagcagatc gccacggctg atgtcgatct catccgtcag caccagggtg
    52981 atcgcttctc cggcaaaggc ttcttcgcga tcaccatcaa aagtcacgat ccgcgcgacg
    53041 tttgattcca caccagaggg cagcactttg acacgttgcc cgacttccac gcgaccggat
    53101 gccagcgttc cggcgtaacc acgaaaatcg agattcgggc gattaacgta ctgcaccggg
    53161 aagcgcattg gctgagcatc caccactcgc tggatctcca cggtttccag cacttcgagc
    53221 agtgtcggac cgctgtacca cggcatactt tcactttgcg atgccacgtt gtcgccttcc
    53281 agtgcagaga gcggcacaaa gcggatatcc agattacccg gcagctgccc ggcaaaggtc
    53341 aaataatctt cacgaatacg ggtgaacgtc tcttcactgt aatccaccag atccattttg
    53401 ttgatcgcca cgaccagatg tttgatcccc aacagtgtgg agataaaact gtgacgacgg
    53461 gtttgatcga gcacgccttt acgggcatcg atcagtaaga tcgccagttc acatgtcgat
    53521 gcgccagtcg ccatattgcg ggtgtactgc tcgtgccctg gggtgtcggc gataataaat
    53581 ttacgcttct cggtagagaa atagcggtag gccacgtcaa tggtgatgcc ctgttcgcgc
    53641 tcagcttgca ggccgtccac cagcagagcc agatccagct tttcgccctg ggtgccgtga
    53701 cgcttactgt cgttatgcag cgatgagagc tgatcttcgt agatttggcg ggtatcgtgc
    53761 agcagacgac caatcagagt acttttgccg tcatcgacgc taccacaggt cagaaaacgc
    53821 agcaggcttt tatgttgttg cgcaatcatc caggcttcga cgccgccttc attggcgatt
    53881 tgttgtgcaa gtgcggtgtt catcttaaaa atacccctga cgttttttca gctccataga
    53941 ccccgcctgg tcgcggtcaa tcacgcggcc ctgacgttca ctggtggtgg aaaccagcat
    54001 ctcttcgatg atttccggca gtgtttgtgc atttgactcc accgcaccgg tcagcggcca
    54061 gcagcccagc gtacggaaac gcaccatccg ttttttaatc acttcgcccg gttgcaggtc
    54121 gatacggttg tcatcaatca tcatcaacat accgtcgcgt tccagaaccg gacgttccgc
    54181 agcgagatat agcggaacaa tgtcgatatt ttccagccag atgtattgcc agatatcctg
    54241 ctcggtccag ttagagagcg ggaagacgcg gatgctttcg cctttgttaa tttgcccgtt
    54301 gtagttgtgc cacagctccg ggcgctgatt tttcggatcc cagcgatgga agcggtcacg
    54361 gaaagagtaa attcgctctt tagcgcggga tttctcttcg tcacggcgcg caccaccgaa
    54421 ggcggcatca aaaccgtatt tgttcagcgc ctgtttcagg ccttcagttt tcataatatc
    54481 ggtatgtttc gcgctgccgt gcacgaatgg attaatcccc atcgccacgc cttccgggtt
    54541 tttatgcacc agcagttcgc agccgtaggc tttagcagta cgatcgcgga actcatacat
    54601 ctcgcggaat ttccagccgg tatcgacatg cagcaacggg aaaggcagcg tacctggata
    54661 aaacgccttg cgcgccagat gcagcatgac gctggaatct ttaccgatag agtagagcat
    54721 caccggattt gagaattctg ccgccacctc gcgaataatg tggatgcttt ccgcctccag
    54781 ttgccgcagg tgagtaagtc gtttttgatc cataaccgtt cctttgcaat accgctattt
    54841 tcttgccatc agatgtttcg actataggga gcgtaagaga acgaatgaaa ttaccaatta
    54901 gaatgagtag ttccttaacg gaataacgat ttggcaaagc taatatcaaa aaatgcttaa
    54961 ggcaccggat ttcgggcgtt taggaagatt tgaaattgtt ttagcgcagc ggcagtttca
    55021 tactatggcg gtaaaaaaat ttgcatggta tttaaggact cactatgttt tccgcattgc
    55081 gccaccgtac cgctgccctg gcgctcggcg tatgctttat tctccccgta cacgcctcgt
    55141 cacctaaacc tggcgatttt gctaatactc aggcacgaca tattgctact ttctttccgg
    55201 gacgcatgac cggaactcct gcagaaatgt tatctgccga ttatattcgc caacagtttc
    55261 agcaaatggg ttatcgcagt gatattcgga catttaatag tcggtatatt tataccgccc
    55321 gcgataatcg taagagctgg cataacgtga cgggaagtac ggtgattgcc gctcatgaag
    55381 gcaaagcgcc gcagcagatc atcattatgg cgcatctgga tacttacgcc ccgctgagcg
    55441 atgctgacgc cgatgccaat ctcggcgggc tgacgttaca aggaatggat gataacgccg
    55501 caggtttagg tgtcatgctg gaattggcag aacgcctgaa aaatacgcct accgagtatg
    55561 gtattcgatt tgtggcgacc agcggcgaag aggaagggaa attaggcgct gagaatttac
    55621 tcaagcggat gagtgacacc gaaaagaaaa atacgctgct ggtgattaat ctcgataact
    55681 taattgttgg cgataaattg tatttcaaca gcggtgtaaa aacccctgaa gcagtaagga
    55741 aattaacgcg cgacagggcg ctggcaattg cgcgcagtca cggaatagcc gcaacgacca
    55801 atccgggttt gaataaaaat tatccgaaag gcactgggtg ttgtaatgac gcagaaatat
    55861 tcgacaaagc gggcattgct gtactttcgg tggaagcgac taactggaat cttgggaata
    55921 aggatggtta tcagcaacgc gcaaaaacag ccgcattccc tgcgggaaat agctggcatg
    55981 acgtaagact ggataatcaa cagcatattg ataaggcact tcctggaaga atagaacgtc
    56041 gctgccgtga cgttatgcgg ataatgctac cgttggtgaa ggagctggcg aaggcgtctt
    56101 gatgggtttg aaaatgggag ctgggagttc taccgcaggg gcggggaatt ctaagtgata
    56161 tccatcatcg catccagtgc gcccggttta tccccgctga tgcggggaac acacatgaat
    56221 gtcggttcag accgtgtttt tacccggttt atccccgctg gcgcggggaa cacagtgctg
    56281 gaccatttca aattcacaat agattcggtt tatccccgct ggcgcgggga acacggaatg
    56341 atatttcaat aaataattat aacaatcggt ttatccccgc tggcgcgggg aacactgtaa
    56401 tagcctgatc tctgatctcc ctcgcctcgg tttatccccg ctggcgcggg gaacacctgt
    56461 cccaggccga ggctgtattt caatcctgcg gtttatcccc gctggcgcgg ggaacacgcg
    56521 atggatcagg gtattacact cgacggcggc ggtttatccc cgctggcgcg gggaacacct
    56581 cagcctcagc cagcgtaaac attcctgtta cggtttatcc ccgctggcgc ggggaacacc
    56641 gcgcgatcgc gtcttcaatg tttacgacct tcggtttatc cccgctggcg cggggaacac
    56701 atgacgctgc aatgtccttt tcctgggcgt cccggtttat ccccgctggc gcggggaaca
    56761 cggcagatcg cagcgtttag ccaacggccc gcccggttta tccccgctgg cgcggggaac
    56821 accgaggcat cagcatgata ttgccggatg taatcggttt atccccgctg gcgcggggaa
    56881 cacgcgatcg cggtggttaa tctgctgaaa atacccggtt tatccccgct ggcgcgggga
    56941 acacgtgacg ctaaacgcgg cgttaaggtc aaggggcggt ttatccccgc tggcgcgggg
    57001 aacacactaa gcatacataa ctgtttttaa acaaatttat tccgcatcaa caatctacca
    57061 actaaattaa aacatttcct tatttttaaa gaacggataa cttattgatt atcaacagga
    57121 agaaaagaaa ccaaacgcaa cccatccaaa tccaccggaa tacgtctatt ttcaccccag
    57181 gtctgaaatt caaaacctga ctcggtattg gtcgcccaag ccatcactac atttccgcaa
    57241 cctgccagtt gagagatttg ctgccagatc atctcccgaa tacgtttgga tgtatctccg
    57301 acatacacac cggcacgcac ttccagtagc cagatagcga gccgtccacg taaacgtgga
    57361 ggaacatttt ccgttacaac tacgatcata ctcatccgcc gcgtcctcga tgaccgctat
    57421 cgcctaacgt ttcaggttca gggatggcag gcggtaacat atccggcgcg ggttgtggtg
    57481 gttcaatttc accagcggca agcacttcct caattaacgg aattaacttg cccgttaatt
    57541 tactactacg gaaaatatcg cgacaggcca acctgacttc tttatctggt tctgcgggtt
    57601 gcctcgctgc tatttcaaat gcctttggca caaccaaatc aaatttaatg atatcggcta
    57661 tgtcataaac aaatgaaagc ggtttgccgc tatggataaa accaatagcg ggcgcatatc
    57721 ccgcagctaa tactgcagct tcagaaatac cgtacaggca tgatgtggca gcactgatgc
    57781 aacgattgac aacatcgcct ttttcccagt ctttaggatc gtatttgcga ccattccatt
    57841 tcacaccata ttgtttcgcc agtaatgcat acgtctggcg aacgcgggat ccctcaattc
    57901 cccgtaactg ctccactgaa cggcgagctg gcggtggctc acgaaaacgt aattcataca
    57961 ttttgcgcac caccttcagg cgtagatctt ccgttaaagc cagctttgcc tggtagagta
    58021 acttatcagc acgcgcccca ccgggttgtc cggaagagta gacgcgaaca cccgcttcac
    58081 cgacccagac cagtagcgtc cccaccgtgg cggccagatg caccgccgcg tgggaaactc
    58141 tcgttcccgg ttcgagcata atgcaggcga ccgatcccac cggaatgtgc gtgcggatcc
    58201 cggttttgtc gatcagcacg aaagcgccgt ccagtacgtc gatttgaccg tactggagga
    58261 agatcatgga ggtgcgatct tttaacggga tcgggctcag cggtacaaac gtcacgcctc
    58321 tgctccaggt ttgatcagca tcagaccaca accgaacgcc cggcttttgc catacccctg
    58381 actgagacgt tgcagaaata acccaggatc ggtgaccgta agcatccccg tataatccac
    58441 actactgaac tggatcagtt gccaggagtt ttcccgccgc agttgttgtt gtcggtaggc
    58501 atcaaccgag gtatccagta gtgtaaaacc acttctttcc ccctgcgctg ccagccagtc
    58561 cagcgccgcc tgttgttgat gcaaccagac atcacttcct tccgcctgtc ccctcacctg
    58621 ccgtttcgcc tccatcagca gatcgtggcg cttgcccgct ttacagattg ttggattcgc
    58681 ccgcaggttg aaacaaagtt gttgtccggt actcagctcg ggcgtaaatg accggcattc
    58741 gatggtgaaa gtttcacttt ccgccgggcg ttcctgcgag aggacaaaaa agcgaaacgc
    58801 gccctggagt tcttcccgac gataaagaaa ttgcctttct ttgccgccag ggaagagatc
    58861 ccacaaccac tgatgcatca catattcacc gcgatccacc agatgcagca actgcgcagg
    58921 cgaaagctgg ccggtatgta aggttattct tgagaggtac atggttcctc cttgctgagc
    58981 cacggcccct gattgatggt gcgttcccca aacagccact gctgacgatt taaaggaaca
    59041 tcccggcggc gtaatatttt gcttgcgacc aaaccatcgt gctccccttc ccaccagcat
    59101 tcatcctgaa gtttcgggag tgagactttc agttcgcgga aacgatcctg atactgttgg
    59161 tatgcgttac gtaagacatc agacgcgttg ccttcgagca gtaacggcgc aagtggtaac
    59221 gccagcggat gactttttcg ccccagataa agcggaaaaa ccggatggcg taaaccgtcc
    59281 tgcaactgtt cgaggctgta aggcgcatcg ggggttgttg ccaccgccac catccaccag
    59341 gcatcggtgt agtagtcgcg ccgggagata atcgcgctca aaagctcagg gtcgctcaac
    59401 tcttcgcaac ggctgaaata acgtgcttta cgcacctctt ttggcatctg gaccgtgtga
    59461 taatcccgtg cccagcgcgg gttacggctg gcgcaaacca ccagtgaata gtggcgatta
    59521 aacgcgttta agcgttcggt atcatcacgc cggatcccta ccccggcagc cagcagcccc
    59581 agcaatgctg agcgcgaagg cagttcatgg gtatgacgca cttcgccggg ggcatcgacg
    59641 ccccaggatg ccatcggccc atgaagctga aaaatcagat attggctcat aagccgcccc
    59701 ttacgcgcag ataaagtcca gcacgtcctt catgcttccc tgtttgttca tcacgtcaaa
    59761 acttgcgtaa tcggtcttct gttcgtagac cgtattcata ttttcgcgaa gagttgtaat
    59821 acgctgcacc gccacctcta actgacgggt gccattaatg ggttcataga aagctgccgc
    59881 cagagaacgc ggttgatcgg tgcctttttc tgccagcgcc caggaggcgt aggcacggct
    59941 ggcaaagctg ttctgtttgc cggttgggga gactttaagt gcggcttccg taaaggcacg
    60001 tatggtctga ttagctaacg cttcgtcacc gccaaggttt tcgaccagca gatctttatc
    60061 aatgcagata taggtgtaga acagtgcaga accgaatccg gtttcaccaa gatgcccggc
    60121 accggcatct tcagaagcct ggcgcaaatc atcaacggcg gtgaaaaaat catcttcgac
    60181 aatcgtttca ctgacaccaa atgcgtgcgc tacctggcag gcggcttcaa cattaaactc
    60241 gggtttattc gccagcatac gaccaaacat ggcaatatct accgccatac ggtctttgcg
    60301 taacaacgcg agatcttcct cttttggcgc gcgcttttct tctgcaagct gatgggccag
    60361 cgcttttacg gcgtcaaatt ctgccgggct gatatggact aactgttcag tttcggcgtt
    60421 agtgagcgga tcttttggtt ttttgtcatt tttagctttc ccaagataat cagcaatttt
    60481 tgccgaccat tcgatggctt ttttatcttc aatccctttc tcaatcagga tagttgccgc
    60541 ctcacgcgca atacgcccac tgcgaatacc aatatggccc gccagtgcct gttcaaaaag
    60601 tgcagaagtg cgccacgcac gtttcagact ttgcgaggaa acgcgcagac gcgttgctcc
    60661 acccagcacc acagtttttg gcgcaccggt atcatcacgg ttaaggttgg cagcagggta
    60721 agcggttaac aaatgaagct gaataaatgt tgtcatgaga tagtccttta ttgattttgt
    60781 tcgttatcgg cgtcgccagc ctggtaatat tccagcgccc agcggatacg gataaattcg
    60841 gttggccgct gctggcggcg gtgatgattg agcaggtcgt cgctctcctg gcaccaacgg
    60901 aagaccccct ctgccagaga gtcaagattg acggaaccgt ttaataatct gactgcgcgg
    60961 cgtaactggc gcagtaattc atccggtgtt tttactgccg acagacgagt aaaacgcccc
    61021 tttgacatta ccgccgccag ctgcgcggca aaaggctgcc gttcatcaat agcttttaca
    61081 ttggcgctaa ctgcggcgac cagcgccagt gcggtaaaac gccactcttg tgcttcaatt
    61141 ttccacaaag tgtgggtctg catcagcaga gaacgaaacc cttctgacaa gcaaacatca
    61201 ttaaccgttg tgctacgccg cagacttgca cgattgccgc gtttagcctg caactcttcg
    61261 cgccatttac gcagcgtggc tttgtgctcc tcttttacaa tactcattca gcagcctcct
    61321 gctttttctc cctggcggct ttagcacttt gcttctccgc cgatgttgtg aaatatttct
    61381 tgcgcgcggt cataacgcgt ttcaaatcaa caggctcata tggattggtg aatacacgct
    61441 cgtcaaaatc ctgacgcgcg aataaccaaa tttccttttg ccatttgccg agtaattcgt
    61501 ccgcatcctg accttcttca atttggcgca ctaacctcag gaagcgatgc tgagttttgt
    61561 tccagaagtc gatatccaca aaactgaaat caccccgtgc accttttgga tcggagaacc
    61621 acgcttcttt caatgcactt cgtaacagac ttagaatccg tgaagccgtt tgcgcagcca
    61681 gacgcagttt cggtatctgg ccttcttttt tcttgagcag cagcgggaaa tggtgttcgt
    61741 accaacagcg cgctttcatg ttgtcgaaat catagccaaa tccccacagg cctacttttg
    61801 cctgtttcag actgctggca ttaaagagtt tcaccaccag cgcgggaagt tccgtattgt
    61861 tttctgattt acccgtttcg ataaggccta accagtcgcg ccagattaaa ccgcccggtt
    61921 gtggtttgac cgagtaaaac tcgccaccct ctttaagtgg tatacggtaa ggcgttaagg
    61981 gatgctgcca catggcataa ttcgcaccgt aatttttggt cgtcatcagg ctcaggagcg
    62041 cgtcgctctg ctcaccgcaa atatcgcagt tgccgactgt cgtggtatta aaatcaatac
    62101 gaatacggcg cggcattccc cagtaagcct ggagtttatt gacctgatca tgggttacca
    62161 ccgcatcggc cagttcgctg gttcgcgtcg ggccaagcca ggggaaaacc agatcgtcaa
    62221 attttttggg tagcggtaag tcggcttcat cctgcggcat cacattgggc cacagtttgc
    62281 gccacaaggg cgtttgttga ttgccttgat attcctgtaa ttcaatcaga gtggtcatcg
    62341 gcccaccgcc gcgcaaaccg gtgcgatagc ctttgccgcc tgatggcgca tttaactgta
    62401 gggagaacag agctaacgcg gaacaatgag ggcatacgtg ttcagtcacg ccacgcttaa
    62461 taaagtggtc tttattaaac ttcgttgttt gagcgccggg aatctcaggc agtagcgaag
    62521 cgacctgaac tttatctccc ttgagcgcct cgaaatcctg cataaatgaa ggggaatctg
    62581 ccccaaactg gaaagcgtgt tctaaggaca gcaatgcttc ccgtagcttt tcagcctcca
    62641 gtccgtcttc ccagatatca tcccaacgac cgtgattttt gggcgcgaag ctggtttgta
    62701 gtagccccag caaaaactgc caggccgcac cctggagatc tgcccgcggc gcagcgatat
    62761 cgacaacatt ttcatcagcc agatcgactg gtgccagctt gcctgtcgtt ccgtctttaa
    62821 aacgaacagg caaccacggg gttgtcagaa gtgaaaacga gttcatacgc atcctctcgt
    62881 aaaaaagacc atcccaggtc ggttgtccat ctttccatcc ttttcggtgt atcaactatt
    62941 cagagagtcc tgtcgcagac atctcaacca accttgccaa tcaatccttc cgtagccgaa
    63001 tacccgcaac tttcgtcatc gttgactaaa atcacgtttg ccatctccgg atcttgtcgc
    63061 tgttcaatgc accacagcct gaacgcctct ccctccagta aagaaaactc atcccgatgt
    63121 tttttccacc agcttcggcg cactctgaca acgctcattt cccatgcgtg agcaccggtg
    63181 gcataaggct tcaccacacc gtcaatacag gtagccagcc acagggaaac agactcctca
    63241 gccagacgtg tcgacagttt ttccgggagg taatcgttga tattggcggc atagccgggc
    63301 ctgaagttca gcacaaactt tttagccatt gcgcgatcgc agtaatattt gcccacttgc
    63361 tcctgctcgc tgcgggcaaa tccttccggc attaccacgt cctcaccgta gacggattca
    63421 ataagaagcc gggctgcgtg tggcatctga atagcacctt gctcacgcag cacacgctgc
    63481 gtcagccaga ttcgcccatg atcgggatag acaaatgcac tgttacgcat ggcactgcca
    63541 aaccattcgt cgccaggaga gtcgtcccag acgggagcca gaatcagcaa ttcagggggg
    63601 gaacgctcgt cctttccgtc acgctttaac tgaccattaa tatcgcggat atgccgctgt
    63661 aatcgtcctg ctcgctgaat cagcaaatca acaggggcca ggtcggagat catttcgtcc
    63721 aggtcaatat ccacgctttg ctcaattact tgtgtacaaa caatgacttg cgacgctctc
    63781 tgtaaactac aatatttacc aaagcgtgcc agtgtctccg tttcaattcg ctggcgatcg
    63841 ctaaaagcaa agcggctatg aaaaagtgaa aggctggaag caggaatgac gccgcgggca
    63901 agcagctgac gatgaacctt aatagcgtca tcgacagaat tccggatcca ggcgatgcat
    63961 ttcccctgac ttaccgccga ttcgatacgc gcaatacact cttgttcact atgaagccaa
    64021 cccacgctga cgctacgctc aacgtctttg cgcgtcgcta cccggtgtga gttcacatcg
    64081 gatttcgtga catgcgtcag ccaggggtaa tcatcctttt ccaggaacgg agcttcttgc
    64141 aggccctctg taccccgcgc aaaggcagcg acgagtttgt cgcgctgctg ttgagataac
    64201 gtggcagaaa gcaaaataac gctgtttccg ccacgcgcct gccgctcgat caacccttca
    64261 agaatgcacg acatgtaagc gtcacaggcg tggatctcat cagccagcag gattttgtta
    64321 ctcagcccca gaagccgcag attattatgt ttgaacggca tcaccgccat catcgcctga
    64381 tccagcgtgc cgacgccaat ttcagccagc aacgccttct tgttactgtc ggcaaaccag
    64441 gccgcacatc cctgactgaa cgtttgttca tccggttctt ctgacccgac taaatcacca
    64501 gaccagagtg attcattaaa gcggtccatt aatgtgcggg cactatgtgc cagcaccagg
    64561 ctggggcggg attctggcga atagaaagca agccaggttt tgaccagccg atcgtacatg
    64621 gcattggccg ttgccattgt tggcagacca aaaaacagcc cctgagcttt ccccgcagct
    64681 atcaacctgt gcgccaggat aagcgccgct tctgttttac ctgcgccagt cacatcttcc
    64741 agaataaata actgcggtcc ttgctggctg atatccagat ccagcacctt ttgctgtaac
    64801 ggtgtcggat tctcaataaa aggaaacagc gtattaattc cgttgaaagg cgcgactttt
    64861 gcttttagag gaaagacggt taaggcgttt tgagcctgaa ttaaagtttt ctgccagtaa
    64921 cctttaatat ccattgggtg tgcgacgcgt ggaaaaaatc gcgttgacga acccgtccag
    64981 tctgcgagca cgacggttgc agagatatac caggaaaggt gttttataag ttcaatgccc
    65041 tcatcatcat cccagaatgc ggggatctct atgagcggaa acagtggttt gatttcaagg
    65101 agaaaatctc gtgctgcagc tttgtcttca ggcagaaaat tatccaattc atccatacga
    65161 tcagggggct gaccatgatg ccctgtcgtc acgggcatcc acatttctat cactcgccta
    65221 agtttgcgcg gagagagcga agatgaagga agtaactcct gacattcact taaataatga
    65281 ttccacaacc agtagcccag cgttgaatga gagatttttt cgtaattttt tctggcacct
    65341 tcctgaatct tgagttcagg gggcaggtaa agttgttgaa aagagcgggc aaattttcca
    65401 atatcgtgcc agcacagcaa ccaggcgaaa aattgagccg cctgttcctt gtcagaaatc
    65461 cctaattgac gaaaataatc agccagcccg aagcaatttc ttttaaccat taaatagccc
    65521 atcgcggcta catccagcga atgccagcaa agaaggtgat agtcgtcgcc cccgtctttc
    65581 tcgccgcgtc gggtttttcc ccagaaatca aaaaaagtca caatattttt atccttcagt
    65641 aaacttaaag gatatttacg
//
LOCUS       DAPGWD010000008        65448 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPGWD010000008 DAPGWD010000000
VERSION     DAPGWD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.008"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            90..608
                     /locus_tag="RHX88_000469"
     CDS             90..608
                     /locus_tag="RHX88_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV2229203.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            624..1163
                     /locus_tag="RHX88_000470"
     CDS             624..1163
                     /locus_tag="RHX88_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDV2229204.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQY
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1258..2835)
                     /gene="guaA"
                     /locus_tag="RHX88_000471"
     CDS             complement(1258..2835)
                     /gene="guaA"
                     /locus_tag="RHX88_000471"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDV2229205.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2904..4370)
                     /gene="guaB"
                     /locus_tag="RHX88_000472"
     CDS             complement(2904..4370)
                     /gene="guaB"
                     /locus_tag="RHX88_000472"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDV2229206.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4532..5902
                     /gene="xseA"
                     /locus_tag="RHX88_000473"
     CDS             4532..5902
                     /gene="xseA"
                     /locus_tag="RHX88_000473"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDV2229207.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5899..6114)
                     /locus_tag="RHX88_000474"
     CDS             complement(5899..6114)
                     /locus_tag="RHX88_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDV2229208.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6183..7655)
                     /gene="der"
                     /locus_tag="RHX88_000475"
     CDS             complement(6183..7655)
                     /gene="der"
                     /locus_tag="RHX88_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDV2229209.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7773..8951)
                     /gene="bamB"
                     /locus_tag="RHX88_000476"
     CDS             complement(7773..8951)
                     /gene="bamB"
                     /locus_tag="RHX88_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDV2229210.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8962..9582)
                     /gene="yfgM"
                     /locus_tag="RHX88_000477"
     CDS             complement(8962..9582)
                     /gene="yfgM"
                     /locus_tag="RHX88_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDV2229211.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9600..10874)
                     /gene="hisS"
                     /locus_tag="RHX88_000478"
     CDS             complement(9600..10874)
                     /gene="hisS"
                     /locus_tag="RHX88_000478"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDV2229212.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10985..12103)
                     /gene="ispG"
                     /locus_tag="RHX88_000479"
                     /gene_synonym="gcpE"
     CDS             complement(10985..12103)
                     /gene="ispG"
                     /locus_tag="RHX88_000479"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDV2229213.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12130..13143)
                     /gene="rodZ"
                     /locus_tag="RHX88_000480"
     CDS             complement(12130..13143)
                     /gene="rodZ"
                     /locus_tag="RHX88_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDV2229214.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13428..14582)
                     /gene="trmG/rlmN"
                     /locus_tag="RHX88_000481"
     CDS             complement(13428..14582)
                     /gene="trmG/rlmN"
                     /locus_tag="RHX88_000481"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDV2229215.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14732..15163)
                     /gene="ndk"
                     /locus_tag="RHX88_000482"
     CDS             complement(14732..15163)
                     /gene="ndk"
                     /locus_tag="RHX88_000482"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDV2229216.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15312..17624)
                     /gene="pbpC"
                     /locus_tag="RHX88_000483"
     CDS             complement(15312..17624)
                     /gene="pbpC"
                     /locus_tag="RHX88_000483"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDV2229217.1"
                     /translation="MPRLLTKRGYWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVKFVMQ"
     gene            complement(17625..22586)
                     /gene="yfhM"
                     /locus_tag="RHX88_000484"
     CDS             complement(17625..22586)
                     /gene="yfhM"
                     /locus_tag="RHX88_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDV2229218.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLAGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGTAEDLLIVRP"
     gene            22793..23638
                     /gene="sseA"
                     /locus_tag="RHX88_000485"
     CDS             22793..23638
                     /gene="sseA"
                     /locus_tag="RHX88_000485"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDV2229219.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24131..24907)
                     /gene="sseB"
                     /locus_tag="RHX88_000486"
     CDS             complement(24131..24907)
                     /gene="sseB"
                     /locus_tag="RHX88_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDV2229220.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25049..26332)
                     /gene="pepB"
                     /locus_tag="RHX88_000487"
     CDS             complement(25049..26332)
                     /gene="pepB"
                     /locus_tag="RHX88_000487"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDV2229221.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26510..26710)
                     /gene="iscX"
                     /locus_tag="RHX88_000488"
     CDS             complement(26510..26710)
                     /gene="iscX"
                     /locus_tag="RHX88_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDV2229222.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26722..27057)
                     /gene="fdx"
                     /locus_tag="RHX88_000489"
     CDS             complement(26722..27057)
                     /gene="fdx"
                     /locus_tag="RHX88_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDV2229223.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27059..28909)
                     /gene="hscA"
                     /locus_tag="RHX88_000490"
     CDS             complement(27059..28909)
                     /gene="hscA"
                     /locus_tag="RHX88_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDV2229224.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28926..29441)
                     /gene="hscB"
                     /locus_tag="RHX88_000491"
     CDS             complement(28926..29441)
                     /gene="hscB"
                     /locus_tag="RHX88_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDV2229225.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29537..29860)
                     /gene="iscA"
                     /locus_tag="RHX88_000492"
     CDS             complement(29537..29860)
                     /gene="iscA"
                     /locus_tag="RHX88_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDV2229226.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29877..30263)
                     /gene="iscU"
                     /locus_tag="RHX88_000493"
     CDS             complement(29877..30263)
                     /gene="iscU"
                     /locus_tag="RHX88_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDV2229227.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30291..31505)
                     /gene="iscS"
                     /locus_tag="RHX88_000494"
     CDS             complement(30291..31505)
                     /gene="iscS"
                     /locus_tag="RHX88_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDV2229228.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31617..32105)
                     /gene="iscR"
                     /locus_tag="RHX88_000495"
     CDS             complement(31617..32105)
                     /gene="iscR"
                     /locus_tag="RHX88_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDV2229229.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32375..33115)
                     /gene="trmJ"
                     /locus_tag="RHX88_000496"
     CDS             complement(32375..33115)
                     /gene="trmJ"
                     /locus_tag="RHX88_000496"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDV2229230.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33234..34037
                     /gene="suhB"
                     /locus_tag="RHX88_000497"
     CDS             33234..34037
                     /gene="suhB"
                     /locus_tag="RHX88_000497"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDV2229231.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34182..35036
                     /locus_tag="RHX88_000498"
     CDS             34182..35036
                     /locus_tag="RHX88_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2229232.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35227..36507
                     /gene="csiE"
                     /locus_tag="RHX88_000499"
     CDS             35227..36507
                     /gene="csiE"
                     /locus_tag="RHX88_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDV2229233.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSFF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36499..37638)
                     /locus_tag="RHX88_000500"
     CDS             complement(36499..37638)
                     /locus_tag="RHX88_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDV2229234.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37798..38688)
                     /gene="hcaR"
                     /locus_tag="RHX88_000501"
     CDS             complement(37798..38688)
                     /gene="hcaR"
                     /locus_tag="RHX88_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDV2229235.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38824..40185
                     /locus_tag="RHX88_000502"
     CDS             38824..40185
                     /locus_tag="RHX88_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDV2229236.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40182..40700
                     /locus_tag="RHX88_000503"
     CDS             40182..40700
                     /locus_tag="RHX88_000503"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDV2229237.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40700..41020
                     /locus_tag="RHX88_000504"
     CDS             40700..41020
                     /locus_tag="RHX88_000504"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDV2229238.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41017..41829
                     /gene="hcaB"
                     /locus_tag="RHX88_000505"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41017..41829
                     /gene="hcaB"
                     /locus_tag="RHX88_000505"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDV2229239.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41839..43041
                     /locus_tag="RHX88_000506"
     CDS             41839..43041
                     /locus_tag="RHX88_000506"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDV2229240.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43138..43560
                     /locus_tag="RHX88_000507"
     CDS             43138..43560
                     /locus_tag="RHX88_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV2229241.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43608..44480)
                     /locus_tag="RHX88_000508"
     CDS             complement(43608..44480)
                     /locus_tag="RHX88_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV2229242.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLLALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44492..45586)
                     /locus_tag="RHX88_000509"
     CDS             complement(44492..45586)
                     /locus_tag="RHX88_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV2229243.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45619..46617)
                     /locus_tag="RHX88_000510"
     CDS             complement(45619..46617)
                     /locus_tag="RHX88_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2229244.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46642..48153)
                     /locus_tag="RHX88_000511"
     CDS             complement(46642..48153)
                     /locus_tag="RHX88_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV2229245.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48176..49159)
                     /locus_tag="RHX88_000512"
     CDS             complement(48176..49159)
                     /locus_tag="RHX88_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDV2229246.1"
                     /translation="MPKKMRTTRNLLLMGTLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49256..52537)
                     /locus_tag="RHX88_000513"
     CDS             complement(49256..52537)
                     /locus_tag="RHX88_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDV2229247.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52655..53848
                     /locus_tag="RHX88_000514"
     CDS             52655..53848
                     /locus_tag="RHX88_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDV2229248.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53912..55165)
                     /gene="glyA"
                     /locus_tag="RHX88_000515"
     CDS             complement(53912..55165)
                     /gene="glyA"
                     /locus_tag="RHX88_000515"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDV2229249.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55493..56683
                     /gene="hmpA"
                     /locus_tag="RHX88_000516"
     CDS             55493..56683
                     /gene="hmpA"
                     /locus_tag="RHX88_000516"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDV2229250.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56728..57066)
                     /gene="glnB"
                     /locus_tag="RHX88_000517"
     CDS             complement(56728..57066)
                     /gene="glnB"
                     /locus_tag="RHX88_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDV2229251.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57127..58461)
                     /gene="glrR"
                     /locus_tag="RHX88_000518"
     CDS             complement(57127..58461)
                     /gene="glrR"
                     /locus_tag="RHX88_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDV2229252.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58451..59164)
                     /gene="qseG"
                     /locus_tag="RHX88_000519"
     CDS             complement(58451..59164)
                     /gene="qseG"
                     /locus_tag="RHX88_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDV2229253.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(59329..60756)
                     /gene="qseE"
                     /locus_tag="RHX88_000520"
     CDS             complement(59329..60756)
                     /gene="qseE"
                     /locus_tag="RHX88_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDV2229254.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61332..65219)
                     /gene="purL"
                     /locus_tag="RHX88_000521"
                     /gene_synonym="purI"
     CDS             complement(61332..65219)
                     /gene="purL"
                     /locus_tag="RHX88_000521"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDV2229255.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILNDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT        15416 a        18126 c        16520 g        15386 t
ORIGIN      
        1 taatctatcc ctttatacgc aatacattta ctttcctctt ttgatgatct taaatgtctt
       61 atttttcgta atgtgtataa caaggaatag tgatgaaatt taaaaaatgt cttctgcctg
      121 tggcaatgtt agcgtcattc actctggcag gatgccagtc aaatgctgac gatcatgccg
      181 ccgatgttta tcaaaccgat caactgaata ccaaacaaga aactaaaacc gttaatatta
      241 tttccattct tcccgcaaaa gttgccgtag acaactccca aaataaacgg aacgcacaag
      301 ccttcggcgc gcttattggc gcagtcgctg gcggtgttat cggccacaac gtcggttctg
      361 gcagcaattc cggaacgacg gcaggggcag ttggcggcgg agctgtaggc gcggcagtgg
      421 gttctatggt gaatgataaa accttagtgg aaggtgtttc tttaacatat aaggaaggca
      481 ccaaagtgta tacctctacc caggtgggta aagagtgcca gtttacgaca ggtttagccg
      541 ttgttattac cacaacgtat aacgaaacgc gtattcagcc aaatactaaa tgtcctgaaa
      601 agagctaata atcaggagga gtcatgaaga aagtttttct ttgcgccatc ttggcctcct
      661 taagctatcc ggctatcgcc tcatcattgc aggatcaact ctctgctgta gcagaagctg
      721 aacagcaagg taaaaatgaa gagcaaaggc agtatgacga atgggtcgcg gagcgcaaca
      781 gggaaatcca gcaagagaag caacgtcgcg caaatgccca ggccgccgct aacaaaagag
      841 cggcaacggc agcggcaaat aagaaagctc gtcaggataa actggacgcc gaagcctctg
      901 cggacaaaaa acgcgatcaa agttatgaag atgagctacg cagcttagag attcagaaac
      961 aaaaactggc gctggcgaaa gaagaagccc gcgttaagcg agaaaacgaa tttatcgatc
     1021 aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca atctgaagct gacgccaaca
     1081 gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt gggcaaagca gaagagaaca
     1141 aatcggacag ctggtttaat taatcgatgt tagtaacttc aagcctatga ttcttgaaga
     1201 taaaaaaccc tctgtagtaa cagagggttt tgttcattca tagtgcaggg attaaaatca
     1261 ttcccactca atggtagctg gcggcttgcc gctgatgtca tacaccacgc gggaaatacc
     1321 gttcacttca ttgataatgc ggttggaaac gcgaccgagg aaatcgtacg gcagatgcgc
     1381 ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc agagagacaa cccagtcata
     1441 cttacgacca tcgcccatta cgccaacgga acgtaccggc aggaacacag tgaacgcctg
     1501 gctgactttg tcgtacaggt ccgctttacg cagttcttca atgaagatgg cgtcagcacg
     1561 gcgcagcagg tcacagtact ctttcttcac ttcacccagc acacgaacgc caaggcctgg
     1621 tcccgggaac gggtgacggt acagcatgtc gtacggcagg cccagctcca gaccaatctt
     1681 acgcacttcg tctttgaaca gctctttcag cggttcaacc aggcccatct tcatctcttt
     1741 cggcaggccg cccacgttgt ggtgagattt gatgacgtgt gctttaccgg ttgcagaagc
     1801 cgcagattcg ataacgtcag ggtagatggt gccctgcgcc agccacttca cgtcttccag
     1861 tttcagcgct tcttcatcga atacttcaac gaaaacgcgc ccgatgattt tacgttttgc
     1921 ttccggatcg ttttcgccag ccagcgctga caggaagcga tcttctgccg gtacgtgaac
     1981 aatgttaaga ccaaagtgat cgccaaacat atccagaacc tgctctgctt cgttgagacg
     2041 cagcaggccg ttgtcgacga atacgcaagt caggttttta ccgatagcgc ggtgcagcag
     2101 cattgcggtt acggaggaat ccacaccacc agagaggccg aggatgactt tatcgtcgcc
     2161 tacctgctcg cggatgcgag ctacagcatc gtcgataatt ttcgctggcg tccacagggc
     2221 ttcacactgg cagatatcac gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg
     2281 agtcacttcc gggtggaact gtacgccata gaagcgtttt tcttcgttag ccataatggc
     2341 aaacgggcag ctttcggtgc tggctacggt gacgaagtcg gacgggatag cggtaacttt
     2401 atcgccgtgg ctcatccaga catcgagcag cggtttaccg tctgcggtca gcgcatcttc
     2461 gataccgcga accagtgcgc tgtcgtttac gacttcaacc tgcgcgtagc caaattcacg
     2521 ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc atggtttgca tgccatagca
     2581 aacgccgaat accggtacgc ctgcttcaaa gacatactgc ggcgcacgcg ggctgttttc
     2641 ttcggtggtg ctttccgggc cgccggaaag aataatgccg cttggattga agtcacgaat
     2701 ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa acacccagct cacgcacgcg
     2761 gcgcgcaacc agttgagtgt actgagaacc gaagtccaga atgaggatgc gatgcttatg
     2821 aatgttttcc gtcattgacg cttattccga ggcaagtgaa acagataata taaatcgccc
     2881 gacatgaagt cgggcgaaga gaatcaggag cccagacggt agttcgggga ctctttagta
     2941 atggtcacgt cgtgaacgtg gctttcctga atgcccgcac cgctgatacg tacaaactcc
     3001 gctttagtac gcagttcgtc gatagtacca cagccggtca gacccataca ggagcgcagg
     3061 ccgcccatct gctggtgaat gatctctttc aggcgacctt tataagctac gcgaccttcg
     3121 ataccttccg gcaccagttt gtcggcagcg ttatcgctct ggaaataacg gtcagaggaa
     3181 cctttggaca tcgcgcccag ggaacccata ccacggtaag atttgtaaga acggccctgg
     3241 tagagttcga tttcacccgg agattcttca gtacccgcca gcatggaacc caccattacc
     3301 gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg agaagcgaat accaccatct
     3361 gcgataaccg gaataccggt gccttccagg gcttcaactg cgtcagcaac ggcagtgatc
     3421 tgcggaacac ctacgccagt aacgatacga gtcgtacaga tagaaccagg gccgataccg
     3481 actttaaccg cgctgcaacc agcttctgcc aaagcgcgtg cacctgcagc tgttgccacg
     3541 ttgccgccga tgatttgcag atccggatat ttagcacggg tttcacggat acgctgcaaa
     3601 acgccttctg agtgaccgtg ggaggagtcg atcagcagaa cgtcaacgcc agcggcaacc
     3661 agcgcgtcaa cacgctcttc gttacccgca cctgcgccaa ctgcagcacc aacacgcaga
     3721 cggccttgct cgtctttaca ggcgttcggt ttacgttccg ctttctggaa gtctttcacg
     3781 gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca gcgctttttc aacgcgtttt
     3841 tcgtgcattt ttgccagcac cacttcacgg gcttcacctt cacgcacggt gaccagacgc
     3901 tctttcggcg tcatgtaaac gctaaccggc tggttcaggt cggtaacaaa acgcacgtca
     3961 cgaccggtga taatacccac cagttcgttt tcttcggtaa cgaccggata gcccgcaaaa
     4021 ccgttacgct cagtcagttc tttcacttcg cgcagcgtcg tggttggcag aacagtctgc
     4081 ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc gaacttcttc tgcctggcgt
     4141 tcaatggaca tgtttttgtg gataaagccg ataccgcctt cctgagccag agcaatagcc
     4201 aggcgcgctt ccgttacggt atccattgct gcggaaagca tagggatatt cagacgaata
     4261 gttttcgtca gctgggtgct gaggtcagca gtattcggca gaacggtaga atgagcagga
     4321 acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga tacgtagcat gggcaatatc
     4381 tcgaccagag tggttaataa atattgccgc ggcattatac agagcgtaac cgattgcatc
     4441 tacccctttt tgcaaaaaat gcttgctatc cccgaagggc gggttactat cgactgaata
     4501 acctgctgat ttagaatttg atctcgctca catgttacct tctcaatccc ctgcaatttt
     4561 taccgttagt cgcctgaatc aaacggttcg tctgctgctt gagcatgaga tgggacaggt
     4621 ttggatcagc ggcgaaatct ctaatttcac acaaccggct tccggtcact ggtactttac
     4681 actcaaagac gacaccgccc aggtacgctg cgcgatgttc cgcaacagca accgccgggt
     4741 gaccttccgt ccacagcatg gacaacaagt tttagttcgc gccaatatta cgctctacga
     4801 gccgcgcggt gactaccaga taatcgttga gagtatgcag ccggccggtg aagggctgct
     4861 gcaacagaag tacgaacagc tcaaagcgaa gttgcaggct gaaggtttgt tcgatcagca
     4921 atacaaaaaa ccacttccct cccctgcgca ttgcgttggt gtgatcacct caaaaaccgg
     4981 tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc gatccttctc taccggtgat
     5041 catctacccc accgccgttc agggcgatga cgcaccgggg caaattgttc gcgccattga
     5101 actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt gggcgcggcg gcggttcgct
     5161 ggaagattta tggagtttta acgacgaacg cgtagcgcgg gcgatttttg ccagccgcat
     5221 tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc attgccgatt ttgttgccga
     5281 tctgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg agccgtaatc agcaagagtt
     5341 actgcgccag gtgcaatcga cccgtcaacg gctggagatg gcgatggatt attatctcgc
     5401 caaccgcacg cgtcgcttta cgcagatcca tcaccgatta cagcaacagc atccgcagct
     5461 ccggctggca cgccagcaaa ccatgcttga gcgcctgcaa aagcggatga gctttgcgct
     5521 ggaaaatcaa cttaagcgta ccgggcaaca gcagcagcgg ttaacacagc ggctgaatca
     5581 gcaaaatcca cagccgaaga ttcatcgcgc gcaaacgcgc attcagcaac tggaatatcg
     5641 tttagcagaa accctgcgcg tacagcttag cgccacgcgt gaacgtttcg gtaatgcagt
     5701 aacgcacctc gaagccgtaa gcccactgtc aacgctggcg cgtggataca gcgttactac
     5761 tgctactgac ggcaatgtac tgaaaaaagt gaagcaagtt aaagcgggtg aaatgctaac
     5821 cacacgtctg gaagacggct ggatagaaag tgaagttaaa aacatccagc cagtaaaaaa
     5881 atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg cgttttttcg atatcaagcc
     5941 atgcccgtgc tgacagaagt aatctactgc accgcaggct tttaacacct gcaacggttg
     6001 atggcagtct ggacaaagag ctttcatttc aataatttcc ccgcaggatg ggcaacgcgc
     6061 atggtcgtta tcctgatcaa gcacatgctg acattgtgga caatgcagtt ccatatgaat
     6121 tcctccggta gccatccctc tacattcata gagggaatgg cagataaaat acttacggat
     6181 aattatttat ttttcttgat gtgcttcatc agacgcttac gtttacgcat ctgggttggc
     6241 gtcagggtgt tacgcttgtt cgcatacggg ttttcccctt ctttgaactg aatacgaatc
     6301 ggcgatccca ttacgtccag cgatttgcgg aagtagttca tcaagtagcg cttgtaggaa
     6361 tcaggcaggt ctttcacctg attaccgtga atcaccacaa tcggcgggtt ataaccacca
     6421 gcgtgggcat atttcagctt cacacgacga ccgcgtacca gcggcggttg gtgatcttca
     6481 acagccatcg tcatgatgcg cgtcagcata gaggtcccca cacgacgggt ggagctgtca
     6541 tacgcttcac gtactgattc aaacaagtta ccaacaccac tgccgtgcaa ggcagagata
     6601 aagtgcacac gagcaaaatc gataaagccc agacggaagt ccagcgtttc tttcacctgc
     6661 tctttcactt cctggctcag gccatcccac ttattcacca caatgacaag tgagcgccca
     6721 ctattgagaa taaagcccag cagcgagaga tcctgatcgg aaataccttc gcgcgcatca
     6781 atcactaaca tcaccacgtt ggcatcttca atggcctgca acgttttgat tacggagaat
     6841 ttctctacag catcggtgat tttgccgcgt ttacgtacgc cagcggtgtc aatgagcaca
     6901 tactcacgtc catcgcgttc cattggaatg tagatactgt cacgcgtcgt gccaggcatg
     6961 tcataaacaa caacgcgctc ttcaccaaga atacggttag tgagtgtaga cttacctacg
     7021 ttcggacgac ccacaatcgc cagtttgatc ggcagacttt gcgggtcgaa gtcgtcttcc
     7081 tcttcttctt cgccgttctc ttccgcttca aattgcgccc agtattcagc gtcttcgtcg
     7141 acttcctctt gcggtgcgag atcttccatc cacggcagca gcacatgctc cagcagactt
     7201 aatacgccac gaccgtgaga cgcggcgatc gggtagattt cacctaaacc aagcgagtag
     7261 aaatcaacca ctgcctgatc gggatccaga ccgtcagttt tgtttgccac caggaaggtc
     7321 ggtttttcac gggagcgcag atgtttggca atcgcttcat ctgctggcat caggcccgcg
     7381 cgcgcatcca ccataaacag tacgacgtcc gcttcttcaa tcgccagcag cgactgttcc
     7441 gccatgcggg tttctacacc gtcttctgtg ccatcaatcc cgccggtatc aatacagata
     7501 aactcacggc cttcaatttc cgcacgaccg tacttacggt cacgagtcag acccgggaaa
     7561 tccgcaacca gcgcatctcg ggtgcgagtt agacggttaa ataacgtgga ttttcctacg
     7621 ttagggcgcc cgacaagcgc gaccacaggt accatgttta aagcctcatt tttataaatc
     7681 atcagacaac gcacgctata ttcgcgtcgt tgttaaaaac aggaaaacgg cccctgtcca
     7741 ggagccgttt tcaaagtgaa cgacagagac gattaacgtg taatagagta cacggttccg
     7801 tcttttgcct ggatcagcag tttgccgtca gcggcaaccg gttcagtctg gaaaccggaa
     7861 ctatcaactt tttgctgggc aacgaaacga ccatcttcga cgttaatcca gtgcagataa
     7921 ccttcactgt caccgaccac caggttgcca ttatacagca ccggagaagt cagcaggcga
     7981 tgcagcagat cgctttgtgc ccacagcgta acgccgccat caatggtcaa cgccatcacc
     8041 cggtcatttt gatcgaccag atagatgcga ttgccgtcga cgatgaaatc attcaccgaa
     8101 cccagttcgc gtttccacat aatctgacca ctgcgcagat caagcgccgt caggttacca
     8161 ttataggcca gcgcgaaaac aacgccgtta acaacgacgg gagtcgtgtc aacatcgctc
     8221 agacggtcaa tttcggtaga accggtcgcc tgggaaatac gctgctgcca aatcatctgg
     8281 ccctgttcca tcagcactgc gctgacgcga ccattatcgc cccccacgac ggccgcacca
     8341 aaagccgttg ccggcgcaga ctcgccacgc aaagagagcg aaggcatatc gaggttaact
     8401 gtccatttga cagcgccgtc agcttcgttc agcgcttgta actgaccgtt actggtgtgg
     8461 attaacacca gaccgtcgct gaccaccggg cgcgaaagtg cttcacccgc gactttagtt
     8521 tgccatgcca cagtaccatc gctggtattc agcgcgtaaa cctgcgcctt ttcgctgcca
     8581 atgtagacat gcccaccaga cacggtcaca ccgccagaaa gtaatgcagg ctctttagag
     8641 aaccagccat ctttctcggc caggctgaca gaccagattt ctttgccatc atccgcattc
     8701 agcgctttta ccaaaccagc gcggtccgct gcatagacaa cgttgtccgc cagtgccgga
     8761 tgaagattgg aatagaagtt gccaatgccg ctaccaacgg aagtactcca cgccgtggtc
     8821 ggcgtaaact ggttttcaac ggttggcaat ggggacatct ttaccacatc ttcttcgctg
     8881 ttaaacagcg aacagccgct taaaagggta acggaaagca gtcctggcag cagtaattta
     8941 cgcaattgca tcgggtccct ctcagatgga caaattatta attttcatct gcatcatttc
     9001 gctcagtgcc ggagtaacat cgcttttcac gcctgcttcc catgcactac gcgcaccttg
     9061 cttatcacct ttgctcagca atgcttcacc acgcaggtcg gcaacaatgg cagcccaccc
     9121 ttcacctttg atggtatcaa gggttttcag cgcggcatca gcctgcttga gctgtacctg
     9181 aacgcgagca agacgcagat ttatcacggc tttgagattt tcatcgctcg tgtctgccag
     9241 cccctgttgt aactgggcgg cagctttctc tagttcattt ttgtcaacaa attgctgcgc
     9301 aagttccaaa gaagccagcg caccataagt atttttattt tcagcagcaa atttttccgc
     9361 cgccgggatg ctatccggtt tgccttcgct cactgcggta accgcatttt gataggcaag
     9421 agaagcggag cgtgcagaat caacctgatg gctgttccag tagcgccagc caatcagtgc
     9481 gccaacgccc aaaatcaccc caacagccag tgctttgcca ttttcagcaa aaaagcgttt
     9541 aaccgcttct acctggtcgt tttcgttctc gtaaatttcc acgctgtcct tctccttcct
     9601 taacccagta acgtgcgcaa atgcgcggct acgctatcct gcgcaactgc cgtttgctca
     9661 ccagagcgca aatccttcac tactgctgtg ccgttagcca cttcagactc acccagcacc
     9721 acagcaacgc gggcacccca tttatcagca cgggcaaact gtttcttaaa gttgccgccg
     9781 ccgtggttgg tcatcaattt cacgcccggt aattcatcac gcagacgctc agctaatgcc
     9841 atagccgcag attgtgtatc agcacctgaa gccaccaggt atatatcgac aacaggatcg
     9901 gctttaaatt ccggattaac ggcctgtact aacaatacaa gacgttcgag gcccatcgca
     9961 aaaccgacag ccggtgttgc acgaccgccc agttgttcca caagaccgtc ataacgaccg
    10021 cctgcacaca cggtgccctg ggagccgaga ctgttagtca cccactcgaa aacggtacgg
    10081 ttatagtaat ccagaccacg caccagacgc tggtttacgg tgtaagcgat ccccgcgctc
    10141 tcaagcagtt tgcacagacc ggcaaaatgc tcacgagatt cctcgtccag ataatcacct
    10201 aatgccggag cgtcgttgag aagcgcctgc acttccggat tttttgaatc cagcacgcgc
    10261 agcgggttag tgtacatgcg gcgtttgcag tcttcgtcca gcttttcttt atgctgctca
    10321 aggaatgcca ccagcgcatc gcggtaattg gcgcgtgctt ccagcgaacc gatagaattc
    10381 agctcaagag ttacgtgttc ggaaataccc agcgcgcgcc accagcgggc ggtgagcata
    10441 atcagttcag cgtcgatatc cggaccttgc agaccgaaaa cttcgcagcc caactgatgg
    10501 aactgacgat aacgcccttt ctgcggacgc tcgtgacgga acatcggccc gatataccac
    10561 agacgctgtt cctgattgta cagaagacca tgctcgatgc cggcgcgtac acagcccgcc
    10621 gtcccttcag ggcgcagagt caggctgtcg ccattgcgat cctcaaaggt gtacatctct
    10681 ttttcaacca cgtcggtgac ttcaccaatc gcacgtttga atagcggggt ctgctctaca
    10741 atcggcaagc ggatttcact gtaaccgtag ctgccgagca cgtttttcag tgtgccttca
    10801 atgcgctgcc agatggccgt ttcgccaggc aggtaatcgt tcatgccgcg aatggcttga
    10861 atgttttttg ccacgtttat tctctttctg aatataaaaa tgaaccctca acgcttccct
    10921 caatgtttcg ggagctatgc gggttcaatc atacacggga agcgaggcgc ttcccatcac
    10981 gttattattt ttcaacctgc tgaacgtcaa ttcgacgcgc ttcgtccagc tgactggctt
    11041 tcgcacgaat gcgtgcttcc agctggtcaa tcatatcgtt gttgtccaga cggtctttgc
    11101 gcacgccatc ttcatagagg ccgcttttct tgttgccgcc ggtgacgccg agtgtagaaa
    11161 ccagcgcctc acctgggcca ttcaccacgc agccgataat cgaaacgtcc atcggagtga
    11221 tgatatcttc caggcgttgc tccagcgcgt taaccgtacc gataacatca aattcctgac
    11281 gcgaacaggt cgggcaggcg atgaagttga tcccgcgcga acggatacgc agcgatttca
    11341 aaatatcgaa accgactttg atctcttcga ccggatcggc cgccagtgat acgcgcagcg
    11401 tgtcgccgat gccttcagac agcagcagac ctaaaccaat ggcggatttt actgccccgc
    11461 tgcgtgcacc accggcttcg gtgatcccca gatgcagcgg ctgatcgatc tgttttgcca
    11521 gcaaacgata agactcaaca gcgaggaaga cgtcagacgc tttcacgctg actttgaact
    11581 gatcgaagtt caggcgatcg agatgatcaa catggcgcat ggcggattcc agcaacgcct
    11641 gcggcgtcgg ttcgccatac ttttcttgca gatctttttc cagcgatccg gcgttaacgc
    11701 caatacggat cggaatgttt ttatcgcgcg cacagtcaac caccatgcga atacgctctt
    11761 cattaccgat attgccaggg ttaatacgca gacaatcgac gccgtattcc gctactttca
    11821 gtgcaatgcg atagtcgaag tggatgtcag ccaccagcgg cacgttaacc tgctgtttga
    11881 tgagtttgaa cgcttctgcc gcgtccatcg tcggtacgga gacacggacg atatcagcgc
    11941 caacgcgttc cagcgccttg atttgattga ccgttgcttc gacgtctgtc gtacgcgtat
    12001 tggtcatgga ctgtacggcg atgggagcac catcgccaat cggcacattc ccaacgtaaa
    12061 tacgtgttga ttttctacgt tgaattggag cctggttatg catgaaaaat ctcccgcgtt
    12121 acccgtctgt tactgcgccg gtgattgttc ggcattgagg gtcagacgcg caacctggtt
    12181 agttctgata aaacgactca gatcgacagg tttcccttga tactggatct gtactgcggc
    12241 tggcgcacca attttcagtt tgtatggtgc ctggccggtt aagttcaaat taccgtcttt
    12301 acgctgcata ccgctaaaca attttttacc ggtagcatca gtgacctcca gccagcaatc
    12361 ggcagtaaag ttcatcacca gcgcattcgg atcagccacc ggcgtggtca cgccagcctg
    12421 atcggttggc aacggcgcag caccatctgg cgttgttgct gccgttggtg ccggggtcgc
    12481 ggcggtatca acatttgcct gcgaaggcga aacaaccgca ttctgttgcg gatcaacagc
    12541 tggtgctggc gcagttacgg caggtgtttg cgtgttggtt gcggtagtat ccacagacgc
    12601 tggcggcgtg gacgttgtag ccgggtctgt agttgtcgac gtatttaacg gaacactctg
    12661 cccctgctcg ctattactgc tcagttccgc cgaagattga tcggccatag tggtgatctc
    12721 ttcctgctga gctttgtggt cttgccacca ccaggcaccg ctcaggccga taaccacaaa
    12781 caacaccagc caggtgaagg tcatcagcca gccgtcacgt tttttgcggc gtttaccgag
    12841 ggaaaaactc tgcataggcg caacttttgc agcccgtagt ggagcctgct tttccagccc
    12901 tggcagcagt tcttcttctg gaatatgtac cagacgcgca taagagcgga tatatccgcg
    12961 caggaatgtt gaagcaagat cggcgggtgc cttatcttct tcaatgtcgc gtaccgtgga
    13021 aaccttcagg caaagtcgct cggcaacggc ctgctgacta agtcctagtt gttcgcgagc
    13081 attacgcagg cgagcgccgg tagtaagtgc ttcattttgg tcgtgcgtgg cttcagtatt
    13141 cattcgctgc aggtacgttt aaatgagaat tgagatgccg gtgaaccatc atgctcaccc
    13201 acaccgcgaa acatccgtta agttaaccgt tatcatacag tataagaccg tctgtgtgct
    13261 cgtgacaaag cccacattca ctaaggctaa cttactgttg catcgttaca tactgcctta
    13321 aagtcagcaa aaacgcaccg ttaatattga ccagacaatt gcaacttaat acttcattca
    13381 taaaagatta cgccacggta cataaagtaa ccgtggcgta atggctatca gaccgcttta
    13441 atgtcgatgg cttcaccctg catccgttta cgcagggtac gtttcgtacg gtcgataaca
    13501 tcgcccgcca actgaccaca ggcagcatcg atatcatcac cacgggtttt acgcacaata
    13561 gtggtgaaac cgtagctcat cagcactttt gagaaacggt cgatacggct gttcgagctg
    13621 cgtccatacg gcgcgcccgg gaaggggttc cacgggatca ggttgatctt acacggcgta
    13681 tctttcagca gttccgccag ttggtgcgcg tgttcagtgc cgtcgttaac gtggtcaagc
    13741 atcacatact caatagtgac acggccctga ttggcgttgg atttctccag ataacggcgc
    13801 accgcagcaa ggaacgtttc gatattgtac tttttgttga tcggcacaat ttcgtcacga
    13861 atttcgtcgt tcggcgcgtg cagggaaatt gccagtgcaa cgtcgatcat atcgcccagt
    13921 ttatccagcg ccggaactac accggaagta gaaagcgtga cgcgacgttt agacaggcca
    13981 aaaccgaaat catcaagcat gatttccatc gccggaacga cgttgttcag gttgagcagc
    14041 ggctcgccca tgcccatcat cactacgtta gtgattgggc gctgaccggt gacttttgct
    14101 gcgccgacga ttttcgccgc acgccacacc tggccgataa tttccgacac ccgcaggttg
    14161 cggttaaagc cctgctgggc ggtggaacag aatttacact ccagcgcaca ccccacctgc
    14221 gaagagacgc agagcgtggc acggtcgtct tccgggatat acaccgtttc gacgcgctga
    14281 tcgccaacgg cgatcgccca tttaatggtg ccgtcggatg aacgctgttc ttcaaccact
    14341 tccggtgcgc ggatttccgc cacctctttc agtttgccgc gcaacacttt gttgatgtcg
    14401 gtcatctcat caaagttgtc gcagcaatag tgatacatcc acttcatcac ctgatcggcg
    14461 cggaagggtt tttcacctaa atctttaaaa aactcccgca tctgctgacg gttgagatcc
    14521 agcaggttga tttttccatc tttcgtggtg acgttttcag gtgtgactaa ttgttcagac
    14581 atatgctatt ccggcctcgt tattacacgt tatggcccct ggagggttga aaaaagaaac
    14641 gccccggtga gcggctgctc gtccgggggc gctgcattgt acaaattctg gcgcacggat
    14701 gccacgtttg cacgcggcat ttacgaaatt attaacgggt gcgcgggcac acttcgcctt
    14761 cgccaaagaa ataagcgatt tcgcgagcgg cagattcgac ggaatcagaa ccgtgggtac
    14821 cgttttcggt caggctgtca gcgtaatcag cgcgcagagt accagccagt gcgtttgccg
    14881 gattggtcgc gcccagcaga tcgcggtgac gctgaacggc gttttcacct tccagcacgg
    14941 aaaccacgat cgggccagag gtcatgaatt caaccagacc atcaaagaac ggttttccat
    15001 cgtgttcagc ataaaagcca cgagcctgtt caacggtcag gtgcagcatt ttggtgccga
    15061 caattttgaa ccctgcagct tcaaagcgcg caaagatatt accaatgacg ttttttgcta
    15121 ccgcgttcgg tttgataatg gaaaaagtac gttcaatagc catttttacc tctgtaaatt
    15181 gttctgttgt tgtctgtacc agcgtacgag atggcgcgga ttataatgag caacagagcc
    15241 gttgactatt gatgaaggta acattttttt aaaataaaat gagttttagc aacaatcagg
    15301 gacaaaacag actattgcat gacaaatttc actgacgcga tttgccctac gtcatccatc
    15361 accagcaatt gataatcacc tttatccgtc aaatgcaggg tcacgttgcg cccgcgttca
    15421 gttaacggtt cgccattcag aaaccaccag cgttcacctg cccctccact tgattgcaac
    15481 ggcaaagtcg cttccgctgc gcccggtaaa cgtttaataa tcgcgccatc gcgaacgcct
    15541 gtcagctgca gagggagctt ggcatcgtga ccgtatggcg ggcaaattgt cgaggccggt
    15601 ggtaagcgca cagcgcgacg ttctgatgcg ggcagccagg gttccagcgg tagcggccag
    15661 acatttatca tttcttgtcg cgcttgcggg caatcagcgg caacacgttt gccattttca
    15721 tccagccaga tggggaaacg aatgccatta atgccttcct gctccggcag taatagagtt
    15781 ggcggctgac tcccgtccag cagccaggtt gccaggcggc ggcgacagtt accgtcacct
    15841 tccggcaaag actgtccgcc cggccagcag ataacgccac gagtgactga gttcgggcgc
    15901 gggtcttccg gcagattcgc actgcgcgac agtaagatat tattgacctg attcaacaat
    15961 ggtacggcac tggcaaagcc aaactgacca acaacgggcg tgccgtccgg tctgccagtc
    16021 cagatcccaa tgacatagcg agcgttaacc ccaatcgccc aggcgtcacg atagccatag
    16081 ctggtgcccg ttttccatgc cagtggggcg acgcgcggca aggcactatc cggcaagggt
    16141 tgcgcttcat cagccataat ccggcgaatg atccacgccg cccccgacga cattaaaggc
    16201 cgttcaagca gcggatcgtc aggctgtaag cgcaatttgc ctgccttgcc gtggcgagca
    16261 aacgcggtat acgctgccgc catatcttcc agttttgcac cagcgccgcc gagaatgagt
    16321 gaaagattcg gcgcagcacc gttgggcaaa tataacggca atccaacatt gcgtaacttt
    16381 gccgcaaacc gtttcggtcc ataggcttcc agcacctgca cagcaggtaa gttcagcgag
    16441 cgcaccagcg cctcgctcat gctgatcggg ccatgaaaac cgctatcaaa gttacctggt
    16501 cgataatcac cggtgcgccg ggggacgtct tgcagcagtg atgccgggtg gatcaagcct
    16561 tcatccagcg ccagaccata aacaaacggt ttgagcaccg atcctggcga tcggatcgca
    16621 ttgaccatat caacatgacc aaagcgtgaa tcatcgttga gatcaaccga tcccacccag
    16681 ccgcgaacac gcatatcggt atgatcaacc acgatcatcg ccagtgagct gcgcggtggc
    16741 aaccgccctt tccagttttg cgccagttct tccagacgtc gttgaagacc ggcatccagc
    16801 gtagtaacga ttttgtcgct tttgctttta ccgagcatca tgcgcgaaaa cagcggtgcc
    16861 agttgcggca tttgtcgggg tgccagccag atgggttctt cccttgactc ttttacctgc
    16921 tcacgggacc acacaccttg cacggccatc cgttcgagca ctttattacg cgcggcttcg
    16981 gcacgctccg gccaacgatc cgggcgaaga cggctgggcg cttgcggcaa aaccgccagc
    17041 attgccgcct cggaatagct taaattcgca ggcgactttc cgagataagc ccaacttgcc
    17101 gcaccgatcc cctgcaacgt accgccaaac ggagcgcggt taagatacaa ggtcagaatt
    17161 tcacgcttag acagatgcca ttccagttgc aacgcgcgcc agagctggcg aattttgccg
    17221 ccaaatgttt tggggtgagg atcaagcaga cgagcaacct gcatagtgag cgtgctgcca
    17281 ccggaaataa cccgtcccga agtgagatct tgccatgctg cgcgcgccac cgagaatgga
    17341 ttcacccccg gatgcttcca gaaccagcga tcttcataat tgatcagcgc ttcaaggtaa
    17401 cgtggagaaa catcttcgat tgttaccgga taacgccaga tgccgtcagc atcggcgaag
    17461 cgccatagcg gcgtaccatc ctgcgccacg accactcgtg cgggattgac ttcatgcagc
    17521 ggtagaggcc agagtttatc tgctccccac gctgcaagaa aaaggagaaa tggcgcagct
    17581 gccagcgtta tccagtagcc gcgtttggtt aacaagcgag gcatttacgg tcggacaatc
    17641 agcagatctt cagccgtgcc ggtcgcccgc cattggggaa catacattga ttccaccatc
    17701 ggttgcggta cctgatacgt tcccggcgtc accgcccgcg ccagatacac cagcgttacc
    17761 ggttggtatt catcaacggc aaccgccgcc acaaagcgat cgtcacggaa ctcaatgtgc
    17821 ttaatactcg cctgctgcat ctggttcagt aagttttgca cttcgccacc actttgctcc
    17881 aggctggcgc taccgttcgc cagattctga ttttccagtt ccagacccgc aggcagcaga
    17941 tccacgacta acgcatccgg cacgctgtta ctggctttta cctgcaacca gaccagcacc
    18001 agatcgccgc tacgtaacga gtccagcgat ttgctcttac catcagtacc aagaatatga
    18061 cgctcgattt gcagcacatt gttcgccggt aaaggtgcgg attgcggata accgctggca
    18121 tccatacgca accataacgg ctgatcgcca ctgtttctca cctgcaaggt ggcaagttga
    18181 tcgctattca gattgctgtt tagcgttttc tcgcctgcca gcggctcagc tgagaaagag
    18241 gtttgcgcct gccatttacc cggtaaatcc tgaatcgtac gggcagccag gaacaacgcg
    18301 ttactttcct gcgtcgatag ccagcgttca ccaaacgcct gctgcgaaag tgtgttcagc
    18361 aaagtgtact gctcatcggg tagtagttta ttttcttcca gcaaggagag cattaacgcg
    18421 ttgtcgcgca gtggactacc gtaatcaccc agccatatcc gctcatcact atttctcggc
    18481 gttttcagcg ccagcacaat cgcttcttca ccacgcgttg catcacccat ggttttcagc
    18541 gcaacgccaa gttgcagcag cggtaaacca gaagcggcat ctgcacgatg ctcccagatt
    18601 tcacgcagcg cacccagtgg tgctttttgc tgacgggcca gcaccagcgc agcgtaagac
    18661 tgaacggcga atttactggc tttgagatta tccgcgtacg ggatcgacat catgcccgga
    18721 tcttgtaaat agcgcagcag acgctcatta ccccggttaa tggcgtctgt cggcacgctg
    18781 taaccctgtt cgcctgcgcg gaccaggaaa tccatcacgt aagccgtcag ccagtactct
    18841 tcgtcaccgt ttttatccca cagcgcaaag ccgccgttat cacgttgcat ttgcagcaaa
    18901 cgggaaatgc cgatatcgac cgatgcacgg cgtttctcat cactgtcgcc tttgatgccc
    18961 aacgcctgca gttgggctgc gttggtataa agtgacggga acaggccgct ggcggtttgc
    19021 tcaagacagc cgtacggata cgcttttaac tctttgatat aacgtgcaat gttcagcggt
    19081 ggtttgccgc tcaacaacaa ttgcccttcc agcgtaacag gcgagaagtt ttgcaatcca
    19141 tccgccggaa tcgcccatgt ctcaccaggc tgtaacgccg taccgtaatt aaccgtttgt
    19201 gccgggaacg ccggacggac gccgattttc cactgcttat gctgatcggc aacggtttca
    19261 cccggtaacg ctaacccgct aatggtggcc tgaatttctc catcgccata acccggcaat
    19321 gctcgcaccg ggataaacag cgtagtacgc acacctggcg ctaattcaac ggctgcgggt
    19381 gaatcgctga ccagttcaag caaaccactg gcggtcaggg caacgttcag tttttgcggt
    19441 ttatcggtaa gattagtgat atccagcgtc agacgcgagg tatcgccact cgccataaag
    19501 cgcggcatgt tcagttcagc aatcaccggt gcggcaacta tcactttgct ttcgttgcta
    19561 ccgaaatcat ctgccgtcca ggcttgcgcc atgacgcgca attcaccgtt aaaatcgcca
    19621 atcggcagtg taaccgagcc ttcgccctgt tcgttgagcg ttaccggcag cgcctgctgc
    19681 gcgacaatat tgacgtgatt gaccggcggt ttaccaccac gtttcagctc atcaccatcg
    19741 ccaccgaaac gcagagctgc cagacgcccc tgaccttcaa taacctgacc gtaaatatcg
    19801 taaatgtctg cgccatagcg tttctgacca aagaacgcct gccacggatc tggcgtgacg
    19861 tagtcagtaa tattcagcac accactatca acggcagaca ccagcacatt cacctgttta
    19921 ggcttctcgc catttttagt gctggcttta attttcacgg ttaatggttg attgggacgc
    19981 atttttgctg gtgtttccag cgccagatcg aggcgacggt tttcatcgcc aagcggcaga
    20041 tgcaacacac caaccgcgcg ttttggcgtc gcggagcgag atttatcgcc aggacgtacc
    20101 accagcgtac ttaaatacag atcatgacga ttccaggttt tatcgaccgg aatcgtcaga
    20161 tccagccctt gagcccgaac atcaatctct tgccaccaca gcggcccttc actggactcg
    20221 accatcgcat aacctttacc cgccgttggc gcggcgatat gcaacttaat ggtgtcgcca
    20281 gggcgataac tggctttatc cagtttcagc gtgacacggt cgggtcgcac tgcgccgcta
    20341 ccgtcgctgt tgtcctgcca gctatagcca gcccagaaac gaacactact gaccgcttca
    20401 ttcggcgctt tgacttccag acgataagcg ccccactcta ccggaaaact gaccttgccg
    20461 gtttcgtccg ctttcagatc cagagtttgt tcattttcga tcagatcttt ttgatcaaac
    20521 tgtgactgcc agccttcatc ttctgaccag ttccagtagt aatcgcggcg ttcgcgaatc
    20581 aggcgcacct gcaagcccga cacggctttt ttcacgcctt gcgcatcgct ataaacgatg
    20641 tcaaaagcgg cgttactgcc ttcatcaaca atgggctgtt tcaccgtgct gtcagtacga
    20701 taatcgtaaa ccgatttcga ggcgaactgc ggacggatcc ccggcaatgc atcggcaggc
    20761 cagatagcct gctcagcgcg gcgcgtcacc gggcgaccgc ccgattccag caaactaccc
    20821 tggaaaataa cctgtaatgg ggaatgcgtt tccttccact ggctttctgt agaaacttcg
    20881 ccgcgccctt tatcatccag cgtcaactga acttcatcca gcgtgcggga aagattttcg
    20941 gcagctatat cgccgaattc aaaaccaggt aaggctgaca cagcttcacg cagtgggcgc
    21001 aggaaaagtt gcccttgcaa agtattacca ttagcaggtg caccatacag gtagtacccc
    21061 accacggaga atttcacttc atctttcggc gttagcgggg ttttctcacc ggtcagattc
    21121 agcgccatgc gctctggcat aaaatcttcg acgtggaaat cccacatccg atactggtta
    21181 tcgcccgtgt tagcgcgaat atgccacata ccggttgccg cattgctatc gagtggccag
    21241 gtaaagtggt agaggccatt ttccggctga ctaacgacgc tcctgagtac ctgcccatcg
    21301 ggtttaatca cgtctaactt gatgggttga ttgggcaacg ctttaccgtc tgcatcacgc
    21361 agcaaaccat tgaggattac cgtttcaccc gggcgataaa gatcgcgtgg gccaaacatg
    21421 aaaaactgtt tgctatagcc tggcgcgcca gcaatgttaa attctgctaa gtccagcgcc
    21481 ggaagtttta aatcgagtag cgttgtctga ccgtctttac gcgccaacag taatgccgcg
    21541 tttttatcat tttccagctg cacatgcccc tgagcgtcac tggttgcctg agtcagagtc
    21601 tgccctttct catttaataa agagacttca attccttgct gggccgcgcc gttttccaga
    21661 ctttgggtaa agatatccag acgattgtga taacggtgag ctgaaacgcc gatatcactt
    21721 aacgtaaaca gcgtcgcggg attactgtaa tcgtaacgtc cagcctgatt catcacagcc
    21781 agatacacgc ccgcctgctg aagcggtttg atatcgccca gcggcagcaa taatttttca
    21841 cgggtgttac gcgcaggatt gagatcaaac cgtccggtgt agaccagatc cgccatctgc
    21901 agcagtttgt ctgactgcca gttcgccagc gaattgcggt attcccattg gctaatgaat
    21961 gctggcagag attctggctt aacgcggaag aagttaacat cgacattatt aacgttgagc
    22021 gccattaccg gcagcccttc aacgacttta ccaggcagca gcgaaccacg gctggcaaaa
    22081 ccgacgctgg gttggatgtc gcgggtagtt atagtttttt cgtaatcttt actgaaggtt
    22141 gcgttgttga gtgctttgac ctccttgcca atagtaacga tcaaatcacg tttcggttcg
    22201 aggtggcgta aacgtaactc tttaagatta tctgacagct cccaggcacc atccactttg
    22261 ccgctttttt tatcgacgac atgaataacg cgtgagaaat cctgatccgg gtcgagaggg
    22321 atggagaacg tcagcaccag cgtggctgca ccatcaagtt ggacttcaga gagatccagc
    22381 aatgtcagca ccttaccggc actctgttgg gccagttttt gtctctccgg cacggagagt
    22441 tttgctgaac tcgcgttttc tgaagaggcc gctttagtaa cttcagaagg cgcatctttt
    22501 ttcaccgctg ttggcgcgtt atcgttgttg tcgcaccctg ccagcgccag cattagcatg
    22561 caggcggcta cgcgtaactt tttcattttt catccctgcc acaatggccc gttagcaacg
    22621 tcgaataatt attatgcgtg agaatttacg ttatgtaatt cagtatcacg cccatacaat
    22681 ccgaatttta gtgagaattg tggctcagac agccaaatca gcctctttcg cttgtcgctt
    22741 ttcactaaac atccgacaat ttagcctaac cccggcaaaa atggagatgc ctatgtccac
    22801 gacatggttt gtaggagccg actggctcgc cgaacatatt gatgacccgg aaattcagat
    22861 tattgatgcc cgcatggcgt cgcctggaca ggaagatcgt aacgttgctc aggagtatct
    22921 gaatggacat attcccggcg cagtgttttt tgatatcgaa gcgctttctg atcacacttc
    22981 cccgcttccg cacatgctgc cgcgcccgga aacgttcgcc gtggcgatgc gtgaattagg
    23041 cgttaaccag gataagcacc tgattgtcta tgacgaaggt aatcttttct cagccccacg
    23101 agcatggtgg atgctgcgca cctttggtgt agagaaagtg tcgattctgg ggggtggact
    23161 tgcaggctgg cagcgcgatg atctgctgtt agaagaaggt gcagtagagc tgccggaagg
    23221 agagtttaac gccgcgttta atcctgaagc cgtggtgaaa gtaaccgatg tattattggc
    23281 aagccatgaa aatacggcgc aaattattga tgcccgcccg gctgcacgtt ttaacgcaga
    23341 agttgatgaa cctcgcccag gtttacgtcg cggacatatt cccggagcac tgaatgttcc
    23401 gtggacggaa ctggtgcgcg aaggcgaact aaaaacgacc gatgaactgg atgcgatatt
    23461 ttttggtcgc ggcgtcagct acgacaaacc aattatcgtc agctgcggct ctggtgtaac
    23521 ggcagccgtg gttttgttag cactcgcgac gctggatgtg ccaaacgtga aactgtacga
    23581 cggcgcatgg agtgaatggg gcgcgcgggc agatttaccg gttgagccag tgaaataagc
    23641 tttatacagg caataaaaaa ccgccgaatt tggcggtttt ttattgctgg tctggttcgc
    23701 ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac
    23761 tcgccagtaa ctctcttttt gtcaagcaaa agagagttat tattgttctg ttagtgtatt
    23821 atccactgcg gccctttccg ccgtctcgca agcgggcgct ggcttaagga aaggatgttc
    23881 cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg cggcaatatc gccagtggtg
    23941 ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga tacggtgctt ctgtatcgta
    24001 cttattgttt ctggtgcgct gttttcccaa gtaaataata accggagtct ctccggcgac
    24061 aatttactgg tggttaacaa ccttcagagc agcaagtaag cccgaatgcc gccctttagg
    24121 gcggcatatt ttagattatc cggttttgtt taaagtcacg caaaaaacca ccccagcgac
    24181 gttcatagaa tggcacaata tgttcggtaa taaagtggct aattcctttt tccccttttt
    24241 tcacctgaca aatatcgatt ggttcatcgc caggtaatgt atcggtcgct acacttcccg
    24301 tcgcctgaat aatttcttcg atatcaccat cggcttcaat gccaataagt aaattaggct
    24361 gtgcctcttc gttctcttta attgaacaaa taaaagcacg cttcaccggc ttaatggttt
    24421 taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg ctctgcgact tccgataata
    24481 tcagcgattc accgccttcc aggatttcct ggctgctcag cggatttccc tcttcaccaa
    24541 tcaacaaact gatttcacgc ggcataaatt ctttaccggt cggcagtttg gcattaagga
    24601 agagcgtttc gccaagggtc atctcaaaca atgtgcgaac gggcattacg acaaatgcct
    24661 gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt gaaaaaagga atgacgctgg
    24721 tgccgtcttc tttttcccag tgctgtaaat caagcgcact atcttcaacc acagcctcgc
    24781 cctgcgccgc cgtaccaggc acccagacag tggattccag tagagtacgg aaaaaggccg
    24841 ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt
    24901 cggacataag aaccacaatt cattcaacgt tcggggcgca aatgcctgat gcgctacgct
    24961 tatcaggcct acaagggatt cgcaatttgt tgaatttgca gaatttgtag gccggataag
    25021 gcgtttacgc cgcatccggc atattagttt acgccgttaa cagattagct atcgtgcgca
    25081 caccaagtcc cgtagcgccc gcagaccact gttcaaccgg cgctttacgg taagtcgccg
    25141 agcagtcgat atgcagccag ccttgctgat agttctcaac aaagtgcgac aggaagcccg
    25201 ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt attgttcagt tcggcaaagt
    25261 tagacggcag ctggctgcgg tggaactccg ccagcggcag acgccagaat ggttcgttct
    25321 cttgtgaggc actcgccagc aaacgaccgg caagcgcatc gtcaaaactg aacagcgcgt
    25381 gataatcatt acccagcgca gttttcgccg ccccggtgag ggtcgccgca tcaatgatca
    25441 tttccggttt ctgcgcactg gcatcaatca gaccatcggc aagcaccaga cgcccttcgg
    25501 catcagtgtt catcacttca acttttttac cgttgcgata ggtgatgata tcgcccagct
    25561 tgaacgcatt gccgctaatc aggttatccg cacagcagag gaacagcttc acgcgcttgt
    25621 tcagtccgcg cgtaatggca aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt
    25681 ccgacttcat cgagtccata aacgcagtct gtttgatgct gtagccgccg gagtcaaaag
    25741 tgataccttt acctaccagg cacgcgtaca ctggcgcttc tttatcgcca gttgggttgt
    25801 aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg tccgacggtg tgcagcccca
    25861 tataaccttg ctcacgcaga tcttcgcctt tggtgatccg ataagtcaca cgatcgcccg
    25921 cgacgttgct gatcagatca acagcacgct gtgccaattg cgatggtccc aattcttctg
    25981 ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat caggcggtta tccagttcct
    26041 ggcgctgggc atcgtccaga tccggccact ccactttacg cgtgcctttc ggggctttgt
    26101 aaccttgcca gaatgcccag cagcgatccg catcccagcc ttcaccgctt aactgaacat
    26161 gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg gatcagcccc agatcgtctg
    26221 ccccgttcaa atgcagggta atgccgtcat tattaatgct gtaagttgct ttttctcccc
    26281 agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat cgcttctgtc attttagtta
    26341 tccttctttg taaaagggcc gccagttggc agccccagga tttttccgct aaatgattcg
    26401 cgttgcagga aggcggcaag tgagtgaagc cccaggagca tagataacta tgtgactggg
    26461 gtgaacgagc gcagccaacg catctgcggc gtgaagcatg acgcggaaat tattcggcct
    26521 cgtccagcca gactaacaaa atcgcttcga ggattttctc gttggatgcc tgcgggtcgt
    26581 cgtcgaaatc ttccagatcg caaatccact gatgcatatc ggtgaatcga accgttttcg
    26641 gatcaagatc gggatacgca tcgtacagtg cctcgccaat ttcgcggcta tcggtccact
    26701 taagtcccat actaacctct gttaatgctc acgcgcatgg ttgatagtgt aacgcgggat
    26761 ttcgactact aaatcttcgt cggtaacgcg cgcctggcag cttaaacggc tttcgggctc
    26821 cagtccccag gctttgtcca gcatgtcgtc ttcctgctct gagctttccg gcagcgagtc
    26881 aaaaccttca cgaacgatgc agtggcaggt ggtgcaagca caggattttt cacaggcgtg
    26941 ttcaatctcg ataccgttac gcagagctgc gtcgagaatg gtttcaccgc tattagcttc
    27001 cagaacagcg ccatcaggac agagatcctg atgaggcaaa ataacaatct ttggcatatt
    27061 aaacctcgtc cacggaatgg cctttcagcg cacgacgaac cgactggtcc atgcggcgag
    27121 cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc ttgttcgatg gcgtcaacgt
    27181 catcgccctg cgccacttca ctcaggtgag cggcagcatc gtcaatgacc tgacgttctg
    27241 cggcgcttaa cagcgcggca tcagcagcca gcgcgccgtg cagactttcc agcacacgcg
    27301 ccgcttctac tttttgttct gccagcattc gggcttttac gtcctgctcg gcatagctca
    27361 ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag accgtaagac ggtttgacct
    27421 gaatagacgc ctcaacgccg gtggatttct ccatcgccgt cacgctcaaa agaccgtcgg
    27481 catcgacctg gaacgtcacg cgaatatgcg caccgccagc cggtagcgcc ggaataccac
    27541 gcagcgcaaa acgcgccagt gagcggcagt cctgcaccag ttcgcgctca ccctgcatta
    27601 catggataga catcgccgtc tgaccatctt taaaggtggt gaaatcctga gcgcgggcca
    27661 ccggaatagt ggtattacgc ggaatcactt tctccaccag gccgcccatc gtttcgaggc
    27721 ccagcgacag tgggatcaca tcaagcagca gcatttcgct gtctggcttg ttacccacca
    27781 gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt atccgggtcg atggaagtca
    27841 gcggtggacg accgaaaaat tcgcctaccc gttcacgcac cagcggcacg cgagtagaac
    27901 cgcccaccat caccacttcc agcacttcat cagcttctac gcccgcgtct ttcagcgcgc
    27961 gacgacaagc cagtaaggtt cgttttacca gtggcgcgat cagttcattg aattgttcac
    28021 ggctgatttc gccctgccag cccgcaacgt taacggtcac ggagtccgca tcgctcaggg
    28081 cgattttggc cgcaatggcg gcatccagca gttcacgctg aacgcggtta tcgctacgat
    28141 caggaatgcc cgcctgttcg cgaatgtaat ccgccagtag atggtcgaaa tcatcgccgc
    28201 cgagcgcgga atcaccgccg gttgccagca cttcaaacac gccgcgactt aagcgcagaa
    28261 tggaaatatc aaacgtcccg ccaccgaggt cataaacagc gatcacgcct tcctgaccgg
    28321 aatccagccc gtaggcgata gccgcagcgg tcggttcgtt aagtaagcgc aggacgtgaa
    28381 ggcccgccag acgcgccgcg tctttggtgc cctgacgctg ggcatcgtca aagtacgccg
    28441 gaacggtgat aactacacca tccagctcgc ctgccagggc ttcagttgcc cgcgccgcca
    28501 gtgctttgag gatgtccgca gaaacgcgca ccgggttcag cagccccgcc gccgtttcaa
    28561 tcatcggcag gccgttttcg ctggcctgga attgataagg cagatgcgga tagcgttgct
    28621 ggatatcagc cagcgagcgc cccatcaggc gtttaacaga actaatggtg ttggcggtgt
    28681 caagcgctgc gttagtacgc gcgtcataac ccaccgaatg cccttgctgt tgatagtgaa
    28741 caacagatgg cagcaggtga cggccttcat ggtcggctaa cgtttcggcc tgaccgctgc
    28801 gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat accagccgcc agacgacgct
    28861 gatgcggcgc agcactcaaa ccaggttcac taatttgtaa taaggccatg tttagcttcc
    28921 agaaattaaa aatcgagcag tttttcttcg agttgttcgg cactgctccg cagtttatcg
    28981 agaaaacgca gcttacgcac ggtatccgcc gccgcgtccc acgcctcgtt gtctaactgt
    29041 tcaaccatca actgatggcg ggtatcaaac atctttttca cacgtttgat aaagctttcc
    29101 agccgcgctt catctttcgc ctgttcgatc tcgtccagct cttcgcgcag ctccaactgt
    29161 tccatcagga acgcggtgtc gcgcacagta tgctgctcgc tggcgagatc aaaaccgtgt
    29221 aaagaaagca aatattccgc gcgcattaac ggatgacgca gcgtttgcca ggcctggtta
    29281 atggttgcag attgctgtac ggcggcgagt tgttccgcct ggcttccgct ggcgaattta
    29341 tcaggatgat actgacgttg tagatcctga aaacgcaggc tcagcgcctg ggtatcgagt
    29401 tgataacggg caggcaagcc aaagagggtg aagtaatcca taacattctc agggctgcga
    29461 tccgccgcga taacggcgaa tcaggtaaaa caaaccccac gcgcaggcga ccacggtggg
    29521 gttatcggta tgcgcatcaa acgtggaagc tttcgccgca accacactca tctttaacgt
    29581 tcgggttggt gaatttaaac ccttcattca ggccttcttt tacgaagtcc agctgcgtac
    29641 cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt cacgcctttg tcttcaaaca
    29701 cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac ataagccata cctgaacatc
    29761 cggaggttct cacgcccaga cgcaggccaa accctttgcc gcggttagcc agaaaggtat
    29821 ttactcgcgc tgctgcactg tcgctcagtg taatcgacat aaccaaacct caactcttat
    29881 tttgcttcac gtttgctttt atagtccgca atggcggctt tgatcgcgtc ttctgccaga
    29941 atagaacagt gaattttcac cggcggcagt tcaagttctt cagcaatatc ggtgtttttg
    30001 atcgcctgcg cttcgtcgag agacttccct ttcacccatt cggtgaccag ggagctggaa
    30061 gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt cttcaatgat accttcatcg
    30121 ttgactttaa tctgcaactt catcacgtcg ccacaggccg gtgcccccac catgccgctg
    30181 ccgacgttct cgtcgttgtt gtcaaaggaa cccacgttac gcggattctc gtaatggtcg
    30241 ataacttttt cgctgtaagc cattataaat tctcctgatt ccgataccga ttaatgatga
    30301 gcccattcga tgctgttcag atccacgccc tgcttgtaca tttcccacag cggagaaagg
    30361 tcacgcagac gaccgatgga tttacgaact aactcgatgg tgtagtcgat ctcttcttca
    30421 gtagtaaaac gacctaaaga gaaacggata gagctatgtg ccagctcgtc gttcagcccc
    30481 agcgcgcgca gcacgtagga cggttcgagg cttgctgacg tacaggcgga acctgaagaa
    30541 actgcgaggt ctttcagcgc cataatcagc gactcacctt caacgtagtt gaagctgacg
    30601 ttgagaatgt tcggcgcacc gtgttccagg tcaccgttca ggtaaacttc ttcgatatct
    30661 ttgatgccgt tccacagacg gttacgcagg ccgcgcagac gttccatctc ggtcgccatc
    30721 tcttcttttg cgatgcgata ggcctcgccc attccgacga tctggtgaac aggcagagtg
    30781 ccggaacgca taccgcgctc gtgaccgccg ccgtgcattt gcgcttcgat gcgtacgcgc
    30841 ggtttacgac gtacatacag cgcaccgata cctttcgggc catagatttt gtgaccggag
    30901 aaagacatca ggtcaacttt caactggctc aggtcgatag gcagtttacc cacgctctgg
    30961 gttgcatcaa cgtgatagat aatgccacga gcacggcaca tttcgccgat agccgcgata
    31021 tcctgcacca cgccgatttc gttatttacg tgcatgatgg aaacaaggat ggtgtcgtca
    31081 cgcatcgctg cttcaagttc tttcaggtcg ataatgccgt tacgctgcgg tgccaggtag
    31141 gtgacttcaa aaccttcgcg ctccagctga cggcaggtat ccagtaccgc tttgtgttcg
    31201 gttttgctgg tgatgatgtg cttgcctttt ttctgataaa agttggctgc acctttgatc
    31261 gccaggttgt cagattcggt tgcaccagag gtaaagacga tttcacgcgg atcagcgccg
    31321 accagatcgg caatctgatt acgggcgata tctaccgctt cttcagcctg ccagccgaaa
    31381 cggtgagaac gagaggccgg gttaccaaag gttccgtcca tcgtcatgaa ctgcatcatt
    31441 ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt agtcgagata aatcggtaat
    31501 ttcattgctc tataaactcc gtacatcact caatgcaagg aatcaggcta ccggctggat
    31561 gtacgaccgt gtttacggag tatttagcac tccggcctga ttctgaattc tttttattaa
    31621 gcgcgtaact taacgtcgat cgcgtcttgt gtgcgggtgc gtggcgcgtc gtgagtatgc
    31681 tgacgaccag acacatccag cacttcctgg ttattaacca gttcgcctaa agtaatgttg
    31741 ttgagaaaac cggtgagacg gtcgctcaaa tcacgccaca gcgcgtgggt caggcattta
    31801 tcgccgccct ggcagccgcc tttaccctga caacgggtgg catctacaga ttcgtcaacg
    31861 gcgctaatca cttcgccaac ggcgatgctg ctggcatctt tgcctaacag ataaccaccg
    31921 cctggtccac gtacgctgga aaccagacca tttttacgca gacgggaaaa cagttgttcc
    31981 agataagaaa gggaaattcc ctgacgttcg gaaatatcag ccaacggtac cgggcccgct
    32041 tcagagttga gcgcaacgtc aagcattgcg gtcacggcat agcgcccttt agatgtcagt
    32101 ctcatgtctt acttcacctc aaactcgccc ctgcccgggg ttttttattg taaaagtggg
    32161 ggtattgcat agcagggtca agtctgacat tcccgagtaa attggtcaac tatttacttg
    32221 actgatttag tcgggtattt aaccttcagt gccatttttt tatcgtggcg tttgtacctg
    32281 ttgtcggatg cggtgtgaac accttatccg acctacggtt ctgtacattg taggcctgat
    32341 aagacgcgtt aagcgtcgca tcaggcaact actgttattc ggccttgtta cctttattct
    32401 gctgctcaat agaagccaga atcccgcgca ggatattcaa ttcctggctt tccgggcgcg
    32461 cacgggtaaa cagacggcgc aatttattca tcacctgccc cggatggttt tcacggataa
    32521 aaccggttgc cagcagcgtt tgttccagat gaccataaaa acgctccaga tcatcgacca
    32581 gcggatacgg cgtctcttca tgctcgacct gctcgccgtt ttcctgagtt gccagccagg
    32641 ccatgcgcac ttcataggca ataacctgaa ccgccatcgc caggttcagc gagctgtatt
    32701 ccgggttagc cgcaatcgcg acatgataat ggcatttctg caactcttca ttggtcaagc
    32761 cgacgcgctc acgaccaaac accagcgcca ccggggtatt tgccgcttca gcgacgcttt
    32821 tcagaccgca ttcgcgcggg tcgagcatcg gccacggcag cgtgcgggag cgtgcgctgg
    32881 tgcccaccac caggctacaa ccggctaacg cttcgtcgag cgtatcgacg atgtgagcat
    32941 taccaatcac atcgctggcc cctgctgcca gggcaatcgc ctgggagtcg ggtttcacca
    33001 gtggattaac cagccacaga ttggttaatc ccattgtttt catggcacgg gcaacagaac
    33061 ccatattgcc ggtgtgtgac gtctccacca gcacaattcg aatattttgc agcattgtct
    33121 ttcttcatct aaagattatt cacgcatctt atcataaaac gaagacagat gccgatctcg
    33181 ctgctatact ctgcgccgtt ttcccgttct ttaacatcca gtgagagaga ccgatgcatc
    33241 cgatgctgaa catcgccgtg cgcgcagcgc gcaaggcggg taatttaatt gccaaaaact
    33301 atgaaacccc ggacgctgta gaagcgagcc agaaaggcag taacgatttc gtgaccaacg
    33361 tagataaagc tgccgaagcg gtgattatcg acacgattcg taaatcttac ccacagcaca
    33421 ccatcatcac cgaagaaagc ggtgaacttg aaggtactga tcaggatgtt caatgggtta
    33481 tcgatccact ggatggtact accaacttta tcaaacgtct gccgcacttc gcggtatcta
    33541 tcgccgttcg tatcaaaggc cgcaccgaag ttgctgtggt atacgatcct atgcgtaacg
    33601 aactgttcac cgccactcgc ggtcagggcg cacagctgaa cggctaccgt ctgcgcggta
    33661 gcaccgctcg cgatctcgac ggtaccattc tggcgaccgg cttcccgttc aaagcaaaac
    33721 agtacgccac tacctacatc aacatcgtcg gcaaactgtt caacgaatgt gcagacttcc
    33781 gtcgtaccgg ttctgcggcg ctggatctgg cttacgtcgc tgcgggtcgt gttgacggtt
    33841 tctttgaaat cggtctgcgt ccgtgggatt tcgcggcagg cgagctgctg gttcgtgaag
    33901 cgggcggcat cgtcagcgac ttcaccggtg gtcataacta tatgctgacc ggtaacatcg
    33961 ttgctggtaa cccacgtgtt gtgaaagcca tgctggcgaa catgcgtgac gagttaagcg
    34021 acgctctgaa gcgttaatga ctcaggcggg tgatgtcact cacccgccct cgcctttcag
    34081 gcgctattct gaaatacttc ctcaccgctt tactttcttt cgtcactctc ccaccatctt
    34141 tccccgatta atggatgata aagaagtcgc aaggaaaggt tatggcactg ccagtgaaca
    34201 aacgcgttcc caaaattctg tttattctct ttgttgttgc cttctgcgtt tatttagtgc
    34261 cgcgcgttgc catcaacttc ttctattatc ctgacgataa aatttacggt cccgacccct
    34321 ggtcggcgga atccgtcgaa tttacggcta aggacggtac tcgtctgcaa ggctggttta
    34381 ttccttcttc gacgggccct gctgacaacg ccatcgcaac catcattcat gctcacggca
    34441 atgccggaaa tatgtccgcc cactggccgc tggtcagttg gttacccgag cgtaatttca
    34501 acgtttttat gtttgattat cgcgggtttg gtaaatcaaa aggcacgccg tcccaggccg
    34561 gattgctgga cgatacgcaa agcgccatca atgtggtgcg ccatcgcagt gatgtaaacc
    34621 cacaacggct ggtgctgttc gggcagagta ttggcggggc gaatattctt gctgttattg
    34681 gtcagggtga tcgtgaaggc atacgtgcgg tgatcctcga ctccacattt gcctcttatg
    34741 caaccatcgc caaccagatg atccccggca gtggctactt acttgatgag agttacagcg
    34801 gcgaaaatta tatcgccagc gtcagcccga tcccgctttt actcattcac ggtaaagctg
    34861 atcacgttat cccatggcag cacagcgaaa agttgtatag cctggcaaaa gagccaaaac
    34921 ggttgatcct aatcccggat ggcgaacaca ttgatgcttt ttccgatcgc cacggcgatg
    34981 tttatcgcga acagatggtg gactttatcc ttagcgcgtt gaatccgcag aactaaccca
    35041 tgatcgctag cacgataatc attcacaaaa ccaccttaag acatgctaat ccactggtca
    35101 gaacagttta agatgagaaa aattctgtga cgcttgccaa catttctgat gattagcatt
    35161 cccttcgtca tttccctgag caaactttag ctattcttat caattatgct tatgggagat
    35221 ctacagatga tgcctacgct tgctccacca tctgtccttt cggctcccca gcgccgctgc
    35281 cagatcttgc tgacgctctt tcagccgggg ttgaccgcca ccacggcaac tttcagcgag
    35341 cttaatggtg tggatgatga tattgccagt cttgatatca gcgaaacagg acaggagatc
    35401 ctgcgctatc atcaactcac actgacggct ggttatgacg gtagctaccg ggttgaaggt
    35461 acagtgctta accaacgttt gtgtttattt cactggctac gacgtggttt ccgtctgtgt
    35521 ccatcattta ttaccagcca tttcaccccc gccctgaaga gtgaactgaa gcggcgcgga
    35581 attgcgcgta acttttacga cgataccaat ctacaagcgt tagtgaatct ctgctcccga
    35641 cggctgcaaa aacgctttga aacgcgcgat attcatttcc tgtgtctgta tctgcaatat
    35701 tgtttgctgc aacaccacgc tggaattacg cctcagttta atccgctcca acgtcgctgg
    35761 gcggaatcct gccttgaatt tcaggtagcg caggaaattg gacgccactg gcagcgtcgg
    35821 gcgctccagc ctgtaccacc tgatgagcca ctgtttatgg cactactttt ttccatgttg
    35881 cgggttcccg atccactgcg ggatgcgcat cagcgagaca gacaattgcg tcagtctatc
    35941 aaacgtctgg taaaccattt tcgtgagctg ggaaatgttc gtttttatga tgaacagggg
    36001 ttatgtgatc agctttatac ccacctcgcc caggcgttaa atcgcagttt ttttgccatc
    36061 ggtattgata ataccctgcc ggaagagttc gccagactgt acccacgcct ggtgcgcacc
    36121 acccgcgcgg cgctggccgg atttgaaagt gaatacggcg tccatctttc tgatgaggaa
    36181 agtggtctgg tcgcggtgat tttcggtgcc tggctaatgc aggaaaacga tctgcatgaa
    36241 aaacagatta ttctactgac cgggaatgat agcgagcgag aagcgcagat tgagcagcag
    36301 ttacgcgaac taacgttact gccgctcaac attaagcata tgtcggtaaa ggcatttttg
    36361 cagacaggcg ctccgcgtgg cgcggcactg attattgcgc cttataccat gccattaccg
    36421 ctcttttcac caccgctgat ctatacggac ctgacgctga caacacatca acaggagcag
    36481 atccgcaaaa tgctcgaatc agcatgaggg aacaactttc gggcgcaaaa acattgccgg
    36541 cagcgccacc agcgccatta cccagaacac gccgtggccc agatattgat acaggaaacc
    36601 ggcgaaaacg gtcatgatag cgatactgcc gcccatcgcg acggcagagt aaaccgcctg
    36661 taaacggatg acttcgctac cctggcgagc agcaatataa cgcatggcgg ccaggtggca
    36721 gaccgtgaag gtgccgcaat gcagaatttg caccactatc aaccacggca acgccgtagt
    36781 tgctcccata atgccccagc gcactacgcc gcaaatcgcc gagatcaaca gcatatcgcg
    36841 tgcactacaa cggcggaaaa gtttattact cagcgcaaag ataatgactt ccgccaccac
    36901 gcccagcgac cacaaatacc ccaccgccga ggccgagtag ccagctgcct gccagtaaat
    36961 ggcgctaaaa ccgtaatagg ccgcatgtgc cccctgcaat aaacaaacgc aggccagaaa
    37021 gcgccagttc tggcgaacca gcgccaacca cgctgaccaa ccggtgctct cctgctggcg
    37081 gcttgcccct tgtggctgaa tcgtcggacg gatgagaaag ccgagcagca tggatgccac
    37141 gcccaacgtc aacagcgcga ggatcacccg ataatcaaac atagtgacca gtttgcccgt
    37201 cagcgccgag ccaatgacaa acgccaccga gccccacagt cgcactttgc cgtaatcaag
    37261 cgggaactgc ttttgccacg tattcgccag tgcatcggtc aacggtacca gcggtgagaa
    37321 aaagaggtta aagccaatca tcaccagcat cagccacgct acgtgcgccc ccgcccagaa
    37381 ggcgacagca aagagaagtg tcagcagtgc cagcacgcgc aaggcggaaa tcaggcggga
    37441 aggatcgctg acgcggggcg cgatgagcaa actcccgaga aaacgggcaa ccagacctgc
    37501 ccccaataac aggccgatgg tttctggcgt taaaccaatc cctttaagcc agacgctcca
    37561 gaaaggtaga aaaatgccgt aactaaaaaa gtatgtgaaa tagccgagcg ccaaccagcg
    37621 cgtggattgc aaaaccatga gtccctcccg tcaggaggcg ttagtctggg gttaatcgaa
    37681 tgttggtgca agttgaaaaa taagaatatt cgagcctgct tcccgatgtc tgtaacgcca
    37741 gttcacaagc actaaaaaac tgagatccgc gacgcatgtt gcaaaaataa tgcgtaatta
    37801 tgccgttacg cttgccaaac gttcctgcac aatggcgatg aaatcgcgta atgccggctt
    37861 catctcccct ttcttccacg ccatcagtaa agcaatggaa ggcacatttc cggcaatggg
    37921 acgaaaaaca acctgtccgg tattaaaatt attcatataa ccgggtatca aagtgacgcc
    37981 cagccccatg cccaccagat tcatggtcac cagaatattc gttgccacct ggacgatatt
    38041 tggctggctg ttttcttgcg caaaccacgc tttaacgatc ggcgcaagcg aaccggaata
    38101 cgccggatcg gtactgacga aattcacgcc atccagttgc gcggcggtga tctctttttc
    38161 atgtgctaaa gggtgatcaa ccggtaacac aaccactaat ggttcgtcaa aaagctccag
    38221 ataatcaatc tccgggctat aaacaggatg gcgcattaag ccgacgtcga gttcaccacg
    38281 gcgaattttt tcctcctgtt gcgtggtgat taaactcacc agctcaatca aggtgtctgg
    38341 ctgtctgaga cgaaacatcg gtaatacttt tggcagtaaa ttcacttccg ccgatggcac
    38401 aaagccaatg gttaattgtc tgtcttcctg aacaattttc cgcgcccgta atttggcatt
    38461 ttccgcttgt tcgaggattg ccagcgcgtc ctggagaaaa cattctcccg ccgctgtcaa
    38521 cgcgactttg cgcttatccc tcaccagtaa cggaacaccg acacagtttt caagatcgcg
    38581 gatctggctg cttaacgaag gctgtgaggt atgcagtttt tccgccgcac gggtaaagtt
    38641 cagtgcctgc gccactgcga cgaaatagcg taaatgccgt agttccatca ccttcccctt
    38701 gttatcgaaa aaacgtctca gctggtagaa attaactatt tcacatatta gcaaccaacc
    38761 agcaacatcc ttatggcaca aaaatagaag gtcaatacat cttatctttc aggattaaaa
    38821 aatatgacca caccctcaga tttgaacatt taccaactga ttgacaccca aaatggtcgg
    38881 gtcactccgc gtatttatac cgacccggat atttaccaac tggagcttga gcgtattttc
    38941 ggtcgttgct ggctatttct cgcccacgaa agccagatcc caaaacctgg tgatttcttt
    39001 aacacctaca tgggggaaga tgcggttgtc gtagtgcgtc agaaagacgg cagcatcaag
    39061 gcgtttctca accaatgccg ccaccgggcc atgcgtgtga gttatgcaga ttgcggcaac
    39121 actcgcgcct ttacctgccc gtatcacggc tggtcttatg gcattaacgg cgagttgatc
    39181 gatgtaccgc tggaacctcg cgcctaccca caagggttgt gtaaatccca ctggggacta
    39241 aacgaagttc cttgtgtgga gagttataaa gggctgattt ttggcaactg ggataccagc
    39301 gcaccgggcc tgcgtgatta cctgggtgac attgcctggt atctggatgg catgctggat
    39361 cgtcgcgaag gcggcaccga aattgtcggc ggcgtacaaa agtgggtgat caactgtaac
    39421 tggaaattcc cggcagagca gttcgccagt gaccagtatc atgctctgtt cagccatgct
    39481 tctgccgttc aggtattagg ggcgaaagat gatggcagcg ataagcgcct cggtgatgga
    39541 caaaccgccc gcccggtgtg ggaaaccgcc aaagatgcgc tgcaatttgg tcaggacggt
    39601 cacggtagcg gtttcttctt tactgaaaaa ccggatgcta atgtctgggt cgatggcgca
    39661 gtttcaagct attaccgcga aacctatgcc gaagcagaac aacgtttagg tgaagttcgc
    39721 gccctgcgcc tggcgggtca taacaatatt ttccccacgc tttcatggct aaacggcact
    39781 gccacactcc gcgtctggca tccgcgcggc cctgatcaag ttgaagtgtg ggcgttctgt
    39841 attactgaca aagccgcctc cgatgaagtt aaagccgctt ttgaaaacag cgccactcgt
    39901 gcttttggtc ctgctggttt tctcgagcag gatgactcgg agaactggtg tgaaatccag
    39961 aaattgctta aaggccaccg cgcccgcaac agcaaactgt gtctggaaat ggggcttggt
    40021 caggaaaagc gccgcgacga cggcattcct ggcattacta actatatctt ttcagaaact
    40081 gccgctcgtg gaatgtacca acgctgggct gatcttctga gtagcgaaag ctggcaggaa
    40141 gtgctcgata aaaccgccgc ttaccagcag gaggtgatga aatgagtgcg caagtttcac
    40201 tagagttaca tcaccgcatt agccagtttc tctttcacga agccagctta ctggacgact
    40261 ggaaatttcg tgactggctg gcgcagctcg acgaagagat tcgttacacc atgcgcacca
    40321 cagttaacgc gcaaacacgc gaccgccgca aaggcgtcca gccaccgaca acctggattt
    40381 ttaatgacac caaagaccag ctggagcggc gaatcgcccg tctggaaacg ggcatggcct
    40441 gggcagaaga gccgccgtca cgcacccgtc acttaatcag caactgccag ataagcgaaa
    40501 ccgacatccc aaacgtattt gctgtgcggg taaattatct gctttatcgg gcacaaaaag
    40561 agcgcgatga aacattctat gttggaacgc gtttcgacaa agttcgccgt ctggaagatg
    40621 acaactggcg cttgctggaa cgggatatcg tcctggatca agcggtaatc acttcccata
    40681 acctgagtgt actgttctga tgaatcgaat ttatgcgtgt cccgttgcgg atgtgccgga
    40741 gggtgaggct ctccggatcg atacctcgcc cgtcatcgcc ctgttcaacg ttggcggcga
    40801 gttttatgcc attaacgatc gttgcagcca tggtaatgcg tcaatgtcag aagggtatct
    40861 ggaagatgac gccacggtgg agtgcccgct acacgccgcc agtttttgcc tgaaaacggg
    40921 gaaagcgtta tgcctgcccg ccaccgatcc gctcaccact tatccagtac acgttgaagg
    40981 tggtgacatt ttcatcgact taccggaggc gcagccatga gcgatctgca taacgagtcc
    41041 atttttatta ccggcggcgg atcgggatta gggctggcgc tggtcgagcg atttatcgaa
    41101 gaaggcgcgc aggttgccac gctggaactg tcggcggcaa aagtcgccag tctgcgtcag
    41161 cgatttggcg aacatattct ggcggtggaa ggtaacgtga cctgttatgc cgattatcaa
    41221 cgcgcggtcg atcagatcct gactcgttcc ggcaagctgg attgttttat cggcaatgca
    41281 ggcatctggg atcacaatgc ctcactggtt aatactcccg cagagacgct cgaaaccggc
    41341 ttccacgagc tgtttaacgt caatgttctc ggttacctgc tgggcgcaaa agcctgcgct
    41401 ccggcgttaa tcgccagtga aggcagcatg attttcacac tgtcaaatgc cgcctggtat
    41461 cctggcggcg gtggcccgct gtacaccgcc agtaaacatg ccgcaaccgg acttattcgc
    41521 caactggctt atgaactggc accgaaagtg cgggtgaatg gcgtcggccc gtgtggtatg
    41581 gccagcgacc tgcgcggccc acaggcgctc gggcaaagtg aaacctcgat aatgcagtct
    41641 ctgacgccgg agaaaattgc cgccatttta ccgctgcaat ttttcccgca accggcggat
    41701 tttacggggc cgtatgtgat gttggcatcg cggcgcaata atcgcgcatt aagcggtgtg
    41761 atgatcaacg ctgatgcggg tttagcgatt cgcggcattc gccacgtagc ggctgggctg
    41821 gatctttaag gaagcacgat gaaagaaaaa accatcatta ttgtcggtgg cgggcaagcg
    41881 gcggcaatgg ctgcggcctc gctacgccag caagggttca ccggtgagct gcatctgttt
    41941 tccgatgagc gacatcttcc ttatgaacga cctccactct cgaaatccat gttgctggaa
    42001 gattccccgc agttacagca ggtgttaccc gctaactggt ggcaggaaaa caatgttcat
    42061 ctgcattccg gtgtaaccat caaaacgctg ggtcgcgaca cacgagagtt agtgttaacc
    42121 aacggcgaaa gctggcactg ggatcagctt tttatagcaa ccggcgcggc agctcgaccg
    42181 ctgccgttgc ttgatgcact gggagaacgc tgctttaccc tacgccatgc cggtgatgcc
    42241 gccagactgc gagaagttct gcagcccgaa cggtcagtcg tgattgtcgg tgccggaact
    42301 attggtctgg aactggctgc cagcgccacg cagcgcagat gtaaggtgac agtgattgaa
    42361 ctggcggcaa ccgtcatggg ccgtaatgca ccaccgcccg tgcaacgcta tcttttacag
    42421 cgccaccagc aggctggtgt gcgcattctg ctcaataatg ccattgaaca tgttgtcgat
    42481 ggtgaaaaag tagaactgac gctgcaaagt ggggaaacgc ttcaggctga tgtggtgatt
    42541 tacggtattg gtatcagcgc caacgagcaa ctggctcgcg aggccaacct tgatactgcc
    42601 aatggcattg tcattgatga ggcttgccgc acctgcgatc ccgcgatctt tgccggtggc
    42661 gatgtggcaa tcactcgtct tgataatggt gcactacacc gctgcgaaag ctgggaaaac
    42721 gccaataacc aggcgcaaat tgccgctgcc gcaatgttag ggctaccgct accgctactg
    42781 ccgccgccgt ggttctggag cgatcagtac agtgataact tacagtttat tggcgatatg
    42841 cgtggcgatg actggctttg tcgtggcaac ccagaaactc agaaggcgat ttggtttaat
    42901 ctgcaaaacg gcgtgcttat cggtgcggtc acgctgaatc aggggcgtga gattcgccca
    42961 attcgcaaat ggatccagag cggcaaaacg tttgatgcga aactgctgat agatgagaac
    43021 atcgcgctta aatcactgta accaggataa ttagcgaata tctcaatgcc tggggcgtgg
    43081 cgaggtgcaa gagtgtgtat tacgtttaaa tcacattatc ttgcaaaggg attggttatg
    43141 aacacactac gttattttga ttttggagct gcccgccccg ttttgttatt aattgcccgt
    43201 atcgccgtgg tcttaatttt cattattttt ggttttccca aaatgatggg ctttgacggt
    43261 acggtccaat atatggcctc gttgggcgcg ccaatgccga tgctggcagc gattattgcg
    43321 gtagttatgg aagtgcccgc cgcgatatta atcgtgcttg gctttttcac ccgtccgctg
    43381 gcggtgctgt ttattttcta cacgctgggt acggcggtga ttggtcacca ttactgggat
    43441 atgaccggcg atgcggttgg gccaaatatg attaatttct ggaagaatgt cagtatcgct
    43501 ggcgcattcc tgctgctggc aattaccggg ccgggggcaa tttctctcga tcggcgttag
    43561 gaaagatgcc ggatgcggcg tgaacgcctt atccggcatt taataaatta cagccactct
    43621 acccgcaacg acatctctga agttgttgat tcacctggtg caagcgcaag aagatcaccc
    43681 ccttccgggc gatgatgatc atccggcgca tggctcatcg gttccagaca gaagaaatca
    43741 aacgcatatc ctttatcaaa cgcagggtcg gaaacaaaga tgaaataaca cggtgcaggt
    43801 ggcgtcgttt ccatgatgat ggcataacct tcctgcggct gctcgatgcg ggcctgacca
    43861 ttccatccgg caaaaccatt gttcacccac tggcgcggca acggcgcggg ctggttaaaa
    43921 tccagttcct gcggtagctg ctcgcaaaac tcacccgcca gccactgctc ccgctccagc
    43981 cagtaaccgc tcgcctgtgc ctgaatccgc gtttgcggcg acaacggaaa ataaggatgc
    44041 cagccggtac caaatggcag cgtctctgcc ccctgattgg tgacagagag cgtcaccgtc
    44101 agcgtatccg ccgttaaatg aaacgcctga cttacccgat agtgatagac accgctgcga
    44161 tgttcataca ccagacacaa actatcatcg ctgtgcgaga cacattgcca ctcgcccagc
    44221 cagccatcgc cgtgtagata gtgcgcatcc cactcaacat tcggttgcag ttgatactca
    44281 cgcccctgcc agacaaaacg attgccgctc acccggtttg caaatggcac cagcggaaaa
    44341 catgaggcat cggttgccac cccgcttttt ttaccaggac gtaagagcgg agtcgtatcg
    44401 cgccagaagc cttcgattac gccgccctgg tcagaaacgt ccagctttag cgacccgtgg
    44461 gataaggtat agatggtcat ccgctaactc cttaatccgg gaagttaatc acaactttcc
    44521 cgcatttgcc gctcgccatc agcgcatagg catcacctgc ctgttccagc gagaagcgat
    44581 gggtaatggc gttacgcggc cacagtttcc agtccgttaa atcgtgggcg catttttcca
    44641 tatggaacag actggtcacc caggagccaa taatccgccg ttgatggtgc atcagatcgg
    44701 cgctgacctc gaattccact tttccggttt caccaatgta aaccacccgc ccccagtcag
    44761 cagtggattg cagtgccagc aagcgacctg cggcattacc ggaacaatcg agcgcaacat
    44821 ccgcgccacc gtgggtaagt tcagcaatta tctgcggcag accttcggtg gtcgctaaat
    44881 agccgtgatc catcacccct aactgttttg ccatcgccag acgttccggc agcatatcaa
    44941 cgccgatgat ccgttttgca ccgcgacctt tcgccagcat catcgccatc atgccgaccg
    45001 gccccagacc gaccaccagc acgttatcgc tgccggaaac ttcgccgcgc aaaatccctt
    45061 cataggccgt gccaacgccg caactgataa acgcaccatc ttcgtagctc agcgcatccg
    45121 gcaggaggat cagatctttt tcttccgcca gcagatattc ggcatgaccg ccgtcacgct
    45181 gccagccgta agccgctttt cctttgccag tacaagagat aggaaagccg cggcggcagt
    45241 tcgggcaaaa accacagcca gaaatgtgat acaccagcac gcgatcaccc tctttaaaat
    45301 ggcgacagcc ttgccccatc gccacaatct gcccgcacgg ttcatgaccg ttgataaatc
    45361 cctggtataa cggtttatcg ggtgccgccg ccgtggcacg gtgttgatga tagatatagt
    45421 ggacatcgct tccgcaaatc ccggaggatt tcattttgat cagtacctgg ttaatccccg
    45481 gcgtcggcac cgcaacttcc cgcagatcga cggttgaatt tcctggtaaa taagctgcca
    45541 gcatcgtttt cataaatccc tcattaacaa tacgattaat tttcatccct gccgcacccg
    45601 cgccaggggc gttaaggttt agcgtttcgc tttactgctt cgctgggtca gcaagatatt
    45661 cgccagcacc gccaccacga tgatgacgcc gcgtaccacc tgctggaaaa aggagttaat
    45721 accgagcagc accagaccgt taccgattag cgtaatcacc agcacaccaa gcaatgtacc
    45781 gaacagggaa ccgcgaccgc cggaaagtgc cgtaccgccg accacgaccg cggcgatgac
    45841 gtcaaactcc agaccgtttg cggcacctgc gttaccggaa ccgaggcgtg ccgccagcaa
    45901 aatgccggtc accgccgcta ataatcccga aagggtaaag ataagaatgc gcactcgacg
    45961 aacgttaata ccgcacaact gcgccgccgt ggcattaccg ccaacggcaa aaaccgagcg
    46021 cccgaaggcg gtttttcggc taatgaacac aaacagcgca aacaacacca tcatgatcag
    46081 cgcggatact ggcacaccga gaaattgtcc gcccagccag tccagcacct cgttttcgtc
    46141 aatcggcact ggcagtgcgt tggtcataaa cagccccatt ccgcgcaggg cgctccacag
    46201 ccccagtgtg gcaacgaaac ttggtacgtt aaacacgccg cgcagcaccc cggcgagcgt
    46261 tcccatcagc gcgcctaaca gcaacaccag cagacaagca atcgccaacg gaacttcaaa
    46321 ttgcagcaaa aatgccaggc acaccgagac aaaagccacc atcggcccaa cgctgacatc
    46381 aatttcaccg gagataataa tcagcgtcat cgcccaggcg gcaatcccaa tggtggcggc
    46441 atcgcgcagc acgttcatct ggttattcaa tgagataaag ccaggcgcgt tcagggagaa
    46501 gaccagataa agaatggcaa tcaccaccag caaaccgatc tcattaatat ggcgactgac
    46561 aaattgtttg agcgagacgc tcttgccctg cggcaatggt aatgacgaag cagacatctt
    46621 caggttcctc ggtaaaattc atcagtgcac agacagaatg gcggacatca gctcatccac
    46681 attgaccggc gagtgaaact cctgcgagaa cgtaccgtgc tgtaacaaca ggatgcggtc
    46741 gcacaccagc ggcaactcct ccacttcact ggagataaac accacgcttt ttccttcggc
    46801 agccagctca cggacaatac ggtaaatctg ctgtttggct tcgatatcga cgccgcgcgt
    46861 tggctcgtcg agcaacaaaa tctggctggc ggcatagacc caacgaccga tcaccacttt
    46921 ttgctgattg ccgccagaaa gcgtgccgat gggcgtttcg ctactggcgg ccttgaccgt
    46981 catccgctgc atcacctctt cggtcaggcg gcggatggtg gaccattgca gtacgccgtt
    47041 ggcgctgatt ttttgccgat tggtcagcac tgtattttcg tcaacgccca accagggaat
    47101 gatccccgct tctttgcggt tttctggcgt atagccaatg ccgcgtttca gcatgtcgcc
    47161 gtaatcgggg cgcgtgattt tctcgccgtt gataacaatt tcgccctgtt catactcctc
    47221 cagcccaaca atcgccttca gcaattcact gcgccctgcc cccagcaggc cagcaatgcc
    47281 aagcacttcg ccgcgacgta acgtaaagct gatatcctcc agcttgggct tatggcgtaa
    47341 tgcacggact tccagcacgg cctgatccac aatttcctga ggggctaccg gcgcaatatc
    47401 aacgtgatca cgccctagca ttagcgacac aatatgatgc gtggaggtgt tttcgagcat
    47461 cacatcgccc gccacctgac catcgcgcat aacggtggca caggaggcaa tgcggcggat
    47521 ttcttccatc cggtggctga cataaatcac cgccacgccc agtgccgaca tctttttcac
    47581 cgcactgatc accagttcaa cttctgcact ggcaagcgag ctggtgggtt catcaagaat
    47641 gaccacgcgc ggctcgccct tcatcacccg tgcaatttcc accagttgct tttgcgccgg
    47701 gcttagcgtt gaaacaagtt gttcaggact aacgtcaacg cccagcgcct gtaagcaacg
    47761 ttgggcatcc tgcgccattt gcaggtaatc aatcatgccg ttgcggcgag gccattgacc
    47821 aaggcagagg ttttctgcca ccgtcagccc ttccaccaga cttaattcct gataaaccgc
    47881 acgaaccccc agttcagcgg cacggcgagt caacgtagct tcgtcacctt ccagtcgcgt
    47941 ctcgccaatc cagatatcac cgctatccgg gcgttcgctg ccggtaagca ttcgaatgag
    48001 agtcgatttg cccgcgccgt ttttacctaa cagcgcacga acttcgcctt tattgagcgt
    48061 gaagttaacg ttatccagcg caacgacgcc ggggtaacgc ttatttcctg ccaccacttt
    48121 tgctaccggg actgcctctg ttgccgtgaa catagggacc tctgcgaatc agcgattagg
    48181 gcagaccatc aacgtgcgtt gccagccact gtttaccatc ttccgttttg gtatagagat
    48241 cgattggcac ctgaatcact ttttcaccgt cggcttgttt attgataacc ttcaatgttt
    48301 gcgcgaaaac agcattgccc attttcttac cggaaatatc cactaccgct ttcagcacct
    48361 gattgttttc cagctcctga gcaatttcgg ttgtcatatc cgaaccgaaa acagcaattt
    48421 ttccggcctg attttgatta cgtaccgctt ttaccgcgcc gagtgtcgca ccgcccgact
    48481 cccccataat ggcgttgaga tccggcgtgg agataatcag tttttcacca acggaaatcg
    48541 ctttatctaa aacagtccct tcctgattag cgacaatttg cgcgccggga acgcgggatt
    48601 ttaatacttc ttcaaatcct ttacgtcgct gcacacaaac ttcaaaggct tcgcaattga
    48661 tgacggcaat tttcggctgg tcaattttat tggcaataaa ataatcggcg gcagcgttac
    48721 ccagtttttt accaaattcc agtggatcgc cgaccagata cgccgagaca tatttatcga
    48781 cacccttttg attaatacag gtgttgtagc aaatcaccgg aatacccgct tcactggcgc
    48841 gacgaacggt acggctactg ccattttcag acactgccga taaaataatg gcatcgacat
    48901 tacgcgccac gagggtatca acaaaggtac tttctttcga aatatcaccc tgggcgttag
    48961 tttcaattaa ctgtacctgt actgaagaat cttttgccgc atcctgaacg ccctgacgca
    49021 ctccagcgta atatccctgg gtatcaaggt atattgcacc aatggtcatt tctttatccg
    49081 cagccctggc aaatagtgcg ctacctaata gcgtacccat taataataga ttacgggttg
    49141 ttctcatttt tttaggcata gtgcctcctg tagggttttt attaacaacg gcttattcta
    49201 attattttgt gatgagcggc agcgcgtgcc gctcaaatat tacagttaat agggtttagt
    49261 gaacgtagtt aaaaataaaa ggcatcacgg tggtgaataa tgccgctttc ggccaggccg
    49321 gattaatcgc cgtcaattca gcgcgagcag attcatattg cgcaacctcc ccgagtcccg
    49381 cggacgccag catcgccacc atcaggcatt tttctttatg ctgctgttgt aaatcgccat
    49441 acagcgacaa caggtcaggt tgtgaaacag caaagaaatc cgcctcaata ctggttttcg
    49501 ccatctcttg cgcccactgt ttcatttcac taaacagttg ctgtgcgatt tgttgttcgc
    49561 ccagcagtcg cagcgccatt ccttgccaga agagataatc aaccggctga tcgttgtaat
    49621 aactgtggat gttaatggtg cgatcgccgg tcgccgccag acgtaaacaa cgcgtcgctt
    49681 cagtttcatc gccctgcgcg ttggcgcata tcgcctgcca gaaccagatg tcgttatcag
    49741 tttgccccgg taaacggcct tcgcttaaat tctccggata atgcagcgcg gcatgaagca
    49801 gttcgctggc ctgctgcggc tgtctggcat caaggtgctg ccaggcgcgt aataactggt
    49861 tgaggataaa ctgactggtg accttccctt ccccgccttc ccacgggtgg aatttgcgcg
    49921 tggcgagaat gtccgccgct ttgtctgcct gacccgtgag atgccacaaa ttgagcaact
    49981 ctgctgtcat gtcgtcgcgt ttcagcgcaa tttccagatt attttccaga cgcgccagtc
    50041 gtttctccgg tgtggctccg cttaacttat caagcaaatc ccgttcgaaa agcagacgtg
    50101 catcctgcgg cgcaagctga taagcattat caagataacg cgcggccagc tcataatcgt
    50161 gttgcttatt ccacgcatgg atcgctaacc cgcgccagcc gtcggcaaac tccggcgaca
    50221 tctctacgca acgttgccat aaggtaatgg ctttgttgta gctacgcttg ttgtagtaga
    50281 agcaggccag taaatgacgg gcaaaccagc actcttcaat actctccagc gccgccactt
    50341 cttccagcgt attcgggaaa cggacaaact gcgggaagac atcaatggct tttgcgacca
    50401 gttcgccgcg ttcggctttc ggcaataaac tcgcctgcag gtaaagcggc agcgtgcgct
    50461 ggcagtccag tgcgttcagc atctctgccg ccagggtggg cattccccag ttaatcagtt
    50521 gcccggcggt catcagagcg ttaacgtcgc gtccctgaca cagaccgcgc cactgcgcga
    50581 gagctgattc gctacgacca tcgaaccagt tcagccacca cagagtggcg ttcagcggat
    50641 aatcgcgcag cagtttctcg cgctgcacac gcgcgttgtc ctgacgacca cttaacacca
    50701 gcagcagatt atgcaggcaa agcacttcct gattggttgg gcaggcgcga agactttgtt
    50761 ggcaaaaatc cagaccagcg tcgaagttac cattacgcgc cgccagtcgt gccaggccat
    50821 aatagccacc ggctttgctg ttgccgctcc agaccgcccg ccagaaatcc tcttcggctt
    50881 gttgatattg tccctgacgt tcgtaagcac tggcgcgaat caaactggcc tgtccgcact
    50941 gcggattttt gttcagcgca tgtgcgcgtt tcagcgcctg agtggcatac gccaccgctt
    51001 gcgggaaatc ggcgcggtta tattccagca tcgccagcgc caggttacag cggtaatcca
    51061 gcggatccag caccacgccg cgcaggtagt aatcgaacgg tgaacggctg gcgtgatgat
    51121 attgctccag atgctgaccg ataaaccagg cttcatctgt actggtaatg tcttgtgctg
    51181 ccagtggcgc tttggcgacg tccggcaacg gcaactcttg cggctgatgt tcctgataac
    51241 tcagtacaat attgccgtcg gcatcggaga gctcaatggt cagcctttca ggattgatac
    51301 cgtgcaacac gccctggatg gcggtcgcag gcatcagtgc cacggcatca tcaagtaacg
    51361 cgttgcattt gccgatttcg cggatcgcca ggcggtatcc gttcaacgga gagatggcat
    51421 acagccccca ctcaatcccc cgctcactac gctggagttt tatcaccgca tcgcgggagg
    51481 cattttgcac catgcccaaa gaatgataag gcaggaaata ctgctcaaaa cgcttctctt
    51541 cgtaagcatc aagccaggta aaatcaggct ggttatcggc aaaaataccg gtcatcagtt
    51601 cgatatacgg gccgttattg tcggtcagac tcttgtccca cgcctggccg aattcactgt
    51661 gtccccagct ccactgtttt ttacctggcg cgatatggtg gttggcaacg tgtagcaaac
    51721 cgccatcttc atcgtgacac cacgcgccaa caaaatcgta ctgtgatttt tcagccatat
    51781 atgaggttgg aacgggcaca tttttatagc gagaaatgtc cactccggcg gagtagtcca
    51841 ctttgtagta agtgccggtg gcgatgggga aagcggagac ggcccgtttg ccgtgatcaa
    51901 acaccgccgt tacatccggc gggaagacgc tctgatgccc ttccccccct ttcactgccg
    51961 ggttggccca ccacaagaaa tgacgcggcg tggcgttgcc gttatagacg cggctggcaa
    52021 tttccaacgc cgcccgttca ggacgcagtg tgaaacctgt catcacctgt aaaccgtgca
    52081 tcggctccgt ttcgccgacc cacaccgtct gtgcgccgtc ttcatgggct tcgagggtga
    52141 aatcaacggg cataaaggtg gtcgggcgat ggtgttgcgg ccagttaaac tcaatcccgc
    52201 cagagatcca cggtcccagc agccccacca gcgcaggttt aatgacttca ttgtgataga
    52261 caaaatcgcg ttgtttcact ttatcccatg cgcgatgcac acgaccgccc agttccggca
    52321 ggatcatcac tttgatgtag tcgttttcca gccacaccgc ctgccaggat ttcagggttt
    52381 tctgctcgct cagcgtatcg gtcacgccgt agggataaac cgcgccggac gatccctgat
    52441 aaacgcgatt ttccaggaac atgggatgta tatcctgcgg cccggtttca taggtcggga
    52501 tctcaacgcg ctcttgccac acttttactg gagtcatggt gccctcaata ttaacaagac
    52561 atactgaatt aaaagatttg tggcagtgta ttgaacaatc tggcaatgtt ttcgcggaat
    52621 aatcacgcaa ttaactaaac aaggtttagt gaagatgaga gcctgcatta acaatcaaca
    52681 gattcgccac cataacaaat gcgtgattct ggaactgctg taccggcaaa agcgcgccaa
    52741 taaatcaacg ctggcccggc tggcgcaaat ttcgattccg gcagtcagta atattttgca
    52801 ggaactggaa agcgaaaaac gggtggtgaa tatcgacgat gaaagccaga cgcgcgggca
    52861 tagtagcggt acatggctga ttgcgccgga aggtgactgg acgctgtgcc tgaacgtgac
    52921 gcccaccagt attgagtgtc aggttgctaa tgcttgttta agtccgaaag gcgagtttga
    52981 gtatttacag attgatgcac cgacgccgca ggcgctgctg tccgaaatcg aaaaatgctg
    53041 gcatcgccac cgtaaattgt ggccggaccg caccatcaat ctggcgctgg caatccacgg
    53101 tcaggttgat ccggtgaccg gcgtgtcgca aaccatgccg caagcgccgt ggacaacgcc
    53161 ggtggaggtg aaatatctgc tggaagagaa gctcggcatt cgggtgatgg tcgataatga
    53221 ctgcgtgatg ctggcgctgg cggagaaatg gcaaaataat tcgcaggaac gggatttctg
    53281 cgtgatcaac gttgattacg gcattggctc gtcgttcgtg attaacgagc aaatttatcg
    53341 cggcagtttg tatggtagcg gacagattgg tcacaccatc gttaatccgg atggcgtcgt
    53401 ctgcgactgt ggacgttacg gctgcctgga aaccgtcgcc tcgttaagtg ctttaaaaaa
    53461 acaggcgcgg gtatggctaa aatcacaacc ggttagtact caacttgatc ctgaaaaact
    53521 gactacagcg cagttaatcg ctgcctggca aagtggagaa ccgtggatca ccagctgggt
    53581 tgaccgcagt gccaatgcca ttggtttgag tctgtataac ttcctcaaca tcctcaatat
    53641 taatcagatt tggttgtacg gtcgcagttg tgcctttggt gagaactggc ttaatactat
    53701 tattcgccag acaggattta acccgttcga ccgcgacgaa ggaccgagcg tgaaagcgac
    53761 gcaaattggc ttcgggcaat taagccgcgc acaacaggtg ctgggaattg gctatttgta
    53821 tgttgaggcg cagttacggc agatttgatg gcgcaataac gtagaaaagc ttcccgaagg
    53881 aagccttttt tcaacagatt aaccgtttcg cttatgcgta aaccggataa cgtgcgcaga
    53941 tgtcgagaac tttacctttg atgcgctcga taacggcttc atcattgatg ctgtccagca
    54001 cgtcacacat ccagccagcc agttctttcg cttctgcttc tttgaagccg cgacgcgtaa
    54061 ttgccggagt acccacgcga ataccggagg tcacaaacgg gctcttcgga tcgttcggta
    54121 cgctgttttt gttgacggtg atgttagcac ggcccagagc ggcgtctgct tctttaccgg
    54181 tcaggttttt atcgaccaga tcaaccagga acagatggtt atcagtaccg ccggaaacca
    54241 ctttgtagcc gcgttccagg aacacttcta ccatcgcttt agcgttttta gcgacctgct
    54301 gctggtaagt tttgaactca ggctccatcg cttctttcag agcaaccgct ttaccggcga
    54361 ttacgtgcat caacggaccg ccctgaccac cagggaaaac ggcagagttc agttttttgt
    54421 acagctcttc gctaccacct ttcgccagga tcaggccgcc gcgcggaccc gccagggttt
    54481 tgtgagtggt ggtagtaaca acgtgagcat gaggaaccgg gttcgggtag acgccagcag
    54541 caaccaggcc cgcaacgtgc gccatatcaa cgaacaggta agcaccgatg ctgtcagcga
    54601 tttcacgcat tttcgcccag tccaccacgc cggaatatgc agagaaacca ccgataatca
    54661 ttttcggctt gtgttctttg gcttgttttt ccagatcggc gtagtcgata tgaccggtag
    54721 catcgatacc gtaaggaacg atgttgtaca gtttaccgga gaagttaacc ggagaaccgt
    54781 gagtcaggtg accgccatgc gccaggttca tacccagaac ggtatcacct ggttccagca
    54841 gcgcggtgta gaccgcaaag ttagcctggg agccggagtg cggctggacg ttagcgtagt
    54901 cagcgccgaa cagttctttc gcacgatcga tcgccagttg ttcaacgata tcaacatact
    54961 cgcaaccgcc gtagtagcgt ttgcccggat aaccttcagc atatttgttg gtcagctgag
    55021 aaccctgcgc ctgcattacg cgcgggctgg tgtagttttc ggaggcgatc agttcgatgt
    55081 gctcttcctg acgtactttt tcctgctcca tagcctgcca cagttcggca tcataatcgg
    55141 caatgttcat ttcacgcttt aacatccgca tctcctgact cagctaacaa taaaattttt
    55201 ggcctttata ggcggtcctg ttggacaacg gcgaacagta taaccgaatc attgtgcgat
    55261 aacaggtctt gacaaaggaa tttacgcaaa cgattacctt caggctacgc aaggctttgg
    55321 agaataaaga gcttgcaacc ggaaacggat ttcttttcag gtttatgatg caaatttttc
    55381 acttcatcac attctttctg aaaaacacca aagaaccatt tacattgcag ggctattttt
    55441 tataagatgc atttgagata catcaattaa gatgcaaaaa aaggaagacc atatgcttga
    55501 cgctcaaacc atcgctacag taaaagccac catcccttta ctggtggaaa cggggccaaa
    55561 gttaaccgcc catttctacg accgtatgtt tactcataac ccagaactca aagaaatttt
    55621 taacatgagt aaccagcgta atggcgatca acgtgaagcc ctgtttaacg ctattgccgc
    55681 ctacgccagt aatattgaaa acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca
    55741 gaagcacacc agtttccaga tcaaaccgga acagtacaac atcgtcggcg aacacctgtt
    55801 ggcgacgctg gacgaaatgt tcagcccggg ccaggaagtg ctggacgcgt ggggtaaagc
    55861 ctatggtgta ctggctaatg tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc
    55921 cagcaaagcc ggtggttggg aaggtactcg cgatttccgc attgtggcta aaacaccgcg
    55981 cagcgcgctt atcaccagct tcgaactgga gccggtcgac ggtggcgcag tggcagaata
    56041 ccgtccgggg caatatctcg gcgtctggct gaagccggaa ggtttcccgc atcaggaaat
    56101 tcgtcagtac tctttgactc gcaaaccgga tggcaaaggc tatcgtattg cggtgaaacg
    56161 cgaagagggt gggcaggtat ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt
    56221 gaaactggtc gctccggcag gtgatttctt tatggctgtc gcagatgaca caccagtgac
    56281 gttaatctct gccggtgttg gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa
    56341 agcaggccac acagcacaag tgaactggtt ccatgcggca gaaaatggcg atgttcacgc
    56401 ctttgccgat gaagttaagg aactggggca gtcactgccg cgctttaccg cgcacacctg
    56461 gtatcgtcag ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat
    56521 ggatttgagc aaactggaag gtgcgttcag cgatccgaca atgcagttct atctctgcgg
    56581 cccggttggc ttcatgcagt ttaccgcgaa acagttagtg gatctgggcg tgaagcagga
    56641 aaacattcat tacgaatgct ttggcccgca taaggtgctg taatttgatg ttgccggatg
    56701 gaaacatccg gcaacccttg acgcggttta aattgccgcg tcgtcctctt caccggtacg
    56761 gatgcgaatg acccgtgcca cgtcaaagac gaagatttta ccgtcaccga ttttgccggt
    56821 ttgcgccgtg cgaataatgg tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat
    56881 ctcaattttc actttcggca gaaaatccac catatactcc gcgccgcggt acagctcggt
    56941 atggcctttc tggcgaccaa agcctttcac ttcggtcacc gtcatgccgg taataccgac
    57001 ttcggccagt gcttcgcgga catcgtccag cttgaagggt tttataatcg catcaatctt
    57061 tttcatgcta ttccttgaaa aggtcgcctg tcttttgatc tgctaaacgt aacacataac
    57121 gccaattcat tccttgaaat cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa
    57181 ttctgtccgg ttgcgccccg ccattctcgc cgcgtgggtg acgttgcctt tggtaatttg
    57241 cagcagctta cgcaaatagt tgagttcaaa ctgattacgt gcctcaacaa aggttggcag
    57301 cgccgtgttt tcaccctcca gcgcctgctc tactaatgca tcgctaataa ccggagatga
    57361 ggtcagcgcc acgcactgtt caatcacgtt gaccaactgg cgcacattac ccggccagct
    57421 cgcggtcatc aggcgtttca tcgcatcagt ggagaacgcg cggacaaacg gtttatgtcg
    57481 ctctgccgcc tggcgcaaca ggtgatttgc cagaagcgga atgtcttctg tgcgctctgc
    57541 cagagccgga attttcaggc tgacaacgtt gaggcggtaa tagagatctt cacggaattc
    57601 cccgcgcgcc atcgcttttg gcaggtcgcg gtgagtggcg gaaataatcc gcacgtcgat
    57661 atcaatatcg cggttgctgc ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag
    57721 tttgacctgc aatggtgcgg gcatatcgcc aatctcatcg agaaatagcg taccgccttc
    57781 cgccgcctgg aataaacctt cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg
    57841 accaaacagc tccgactcca gcaattgctc gggcaatgcg ccacagttaa tagcaataaa
    57901 tggtttgctg ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt
    57961 gccgctctga ccgttaatca aaacgctgac gtctgattgc gccaccagcc gcgcctgttc
    58021 cagcaaacgc agcatcagcg ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt
    58081 ggctggcgcg gattgctcca gcgcatcgtc aattgcctga tatagcgcgt ctttatcgac
    58141 aggcttggtg aggaaactaa aaacgccctg ctgtgttgca gcaacggcat cgggaataga
    58201 accatgcgcg gtaagaataa ttaccggcat tcccggctgc actttctgaa tttcagcaaa
    58261 cagctgcata ccgtccattt catccatccg caggtcgctg ataactaaat ctactttttc
    58321 gcgattcagt acccgtaatc cttcagcgcc actttccgcc gtgaccacac tgtagccttc
    58381 gctggtcagg cgcaggccaa gcagtttcag caatcccgga tcgtcatcga ccaataataa
    58441 atgcgcaggt ttatggctca tcaggagtga cctcatgggt ggatggcgcg ggcttttcac
    58501 tctcgtgcgg cgtatccggc gagaaatttc cagccggttt gcgggtcgag agctggcgtt
    58561 caatatcagt caggttttcc agcttgcggg tggtgagttc cagttgctgc tgtagaacgt
    58621 gatgttgctg gcgcaatgta tccagctcgc tgtcgctggc ctgctggagc ttgccgtagc
    58681 gttggcgctc ttccgccagt tgcagttgca gcgcctgacc gtcgcgccag agttgataca
    58741 gagggcgaac ctgtgccggg atctcggtac ttagcgcatc aatgcgcgcg accagctggc
    58801 ggcgctcata cggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt
    58861 gccagctgcc gtcgtcatat tggcgagctt gctgacgtga ctgcgcaggc attaaacgat
    58921 cagcacaatc catcgcccgc agccagtaaa gcggattggt ttcggttgat ttaccttgca
    58981 gcgcccagat gtcgctacat tcagtagaaa gatagtcagc cagttgataa accggaattt
    59041 tttcttctgc tggcgtatca atggctggct tattgtgact ctgcacgcaa cccagcaatg
    59101 ccagacatgg cagccctgcc agccacagcc gtcggggcaa taatcgttga aaaatgtgtc
    59161 gcatattcac cagacttaat gcctatccca gtgggcgtaa ttattgcagg cagtttggac
    59221 atggacagcg cggagaaacc ggagcgtaca cgtcgtacgt gaggatttcg agcactgccc
    59281 ggggccgaac tggcaaataa aatagcctga tgggataggc tgttagcatt atttcgtgtt
    59341 tttcgacgac ggcaattcaa tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata
    59401 cagttcccct tgcatacggc gaatacaatc cctggcaatg cttaatccca gaccgctgcc
    59461 cttcaccgcc ccttttcgct ggtggcttcc ttgaaaaaag ggttcgaaga tcatggcgcg
    59521 ttcctcttgc ggaatgggtg tgcctgtatt gatgacatca atataaaccc gtgcaccatg
    59581 taagctgctg cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg cattggagta
    59641 aagattatcc agtacgctca tcagcagcat tggctccgcc aggcaagctg ttgctttgag
    59701 atcgacgtcg gtatgcatca ttttagcccg tgcgggcagg ctatgagcag aaactactgt
    59761 ctccaccagc ggtgctaact caacattctc cagttccacc gcactgtccg cttgtttacg
    59821 gttgtaatca agcagttgtt cgatcagttt ttgcaaattg cggctgctgc tatcaagaat
    59881 gctcaccacc tctttttgct ctggcgtaag cggcccgaca acctggtcag ccagtaattc
    59941 agtgccctcg cgcatactcg ccagtggcgt ttttaattca tgagataaat gtcttaaaaa
    60001 ttgatggcgt tgggattcca gccatgacag gcgctcactt aaccaaagaa tacgttgccc
    60061 aaccgagcgt aactcgctcg gtccactgaa cgaaacgcta ttgcccagag aacgcccttc
    60121 ccccagacgg ttgatcatgc gctcgatatt tttcaccggc ccgataatca tccgcgtgaa
    60181 aagtagcacc atcaccagac tcaccagaaa tagcaccagc gattgccaac caaaatattg
    60241 cccacgttcg gcgatttcac gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt
    60301 ggcctgcacc atttcggtat tggcactggc aaaggcttca agacgcgcgg cggcggcagc
    60361 atcgggacca ctgttgttac actgaagttg ggccagattg ttcaaatcct gacgcaatgc
    60421 ctggtaaagt ttatcgtctg gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg
    60481 cttgcgctgg ctttgataaa ccttcgcaag cgtagggtca tccagcacgc aatactgacg
    60541 gtagctacgc tccatctcca gcgccgcgtt ggtcatcgct tcactgcgcc gggcgtcaat
    60601 aagcgtggtg cggttgacca gcgccgcctg gtcactaagc gcattcaggc tttgccaggc
    60661 ttgccatgcc agcaccaaca ggggtagcag aatcagcaaa aatgccagca ttaccagttg
    60721 tcgtaatgag cggggaaaaa cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa
    60781 tagcctgatg ctaaccgagg ggaagttcag atacaacaaa gccgggaatt acccggcttt
    60841 gttatggaat aaggcggtgc ctaactcgac gtttcgcccg atggttgata tagctacgct
    60901 gatatcagaa gttggacggc aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg
    60961 tcccgaaggg gctgacataa gtcggtgaat gagccactgc ttactattat gcagtaactg
    61021 tgccaataaa gaaaatagtt tggtaacgta ctgattatac gttgctttga gggatttatg
    61081 gcttcttcgc tatttgaatg taaatcagcc actgtgtcgc taaaaagaga caacttaaga
    61141 taaacttatt agattacaat aaaatcaatc atttaagtgt cgccagaaag cgacacggta
    61201 aaccacccat tgtcgtgatt tacagacaca aaaaagctcc cggagttggg agcttatgat
    61261 agtggttggt gcttaatgcc ggatgcggcg taaatgccgc atccgggctg caaaaccaat
    61321 gaactgatga cttaccccaa ctgcttacgc gcattgcgga aaatgcgcat ccatgggcca
    61381 tcctcgcccc agttttccgg atgccaggag ttgctgacag tacggaaaac acgttccggg
    61441 tgcggcatca taatggtgac tcgaccactt tcagtcgtga ctgccgtaat accgttcgga
    61501 gaaccgttcg ggttagccgg gtaggtttca gtgactttgc cgaagttatc gacatagcgc
    61561 agtgccacca gccctttgct ttccagtgcc gccagatgcg ccgcatcacg cacttccacg
    61621 cgcccttcac cgtgagagac agcaatcggc atttgcgagc ccaccatccc ctgcaacagc
    61681 agcgacgggc tttgggtcac ttcaaccagg ctgaaacgcg cttcaaagcg atcggaggta
    61741 ttgcgcacaa aacgtggcca caactcacta cctgggatca gttcacgcag attagacatc
    61801 atctggcaac cgttacatac ccccagcgcc agcgtttgcg gacggtggaa gaaggttgca
    61861 aactcatcgc gtacacggtc attgaacagg attgacttcg cccaaccttc accggcaccc
    61921 agcacatcac cgtaggagaa accaccgcac gcgaccaggg cgtggaaatc ttccaggccc
    61981 gtgcgtccag ccagcaggtc actcatatgc acgtcgatag catcaaagcc tgcacggtgg
    62041 aaagctgccg ccatttcaac atgcgagttc acgccctgct cacgcagtac ggcaactttc
    62101 gggcgcgcgc cagtggcaat aaacggtgct gccacatctt cgttgatatc gaacgacagt
    62161 tttacattca ggcccggatc ggcgtcgttt gatttcgcct ggtgctcctg gtcagcacac
    62221 tccgggttgt cacgcaggcg ctgcatctgc caggtagttt ctgcccacca gacacgcaac
    62281 gttgtgcggc tttcgctgaa tacagtctgc ccgttggcgg taatcacaaa acggtcaccg
    62341 gaaaccgcct gcccaacata atggacgcaa tcagcaagcc catgctgtgc cagtacggac
    62401 tcgaccgctt cacggtcagc ggcacgaacc tgaatcaccg cacccagttc ttcgttaaac
    62461 aacgccgcca ggcgatcgtc acccagagag ccgatatccg catcaatgcc acaatgacca
    62521 gcaaaggcca tttccgccag tgtgaccaac agaccgccat cagagcggtc gtgatacgcc
    62581 agcagcttac gctgtgcaac cagcgcctga atcgcgtcat agaagccttt cagttgcgcg
    62641 acatcgcgta catctgccgg tttgtcgcca agctgacgat aaacctgcgc cagcgccgtt
    62701 gcgcccagcg cgttattgcc tttgcccaaa tcaatcagca gcagtgcgtt atcttcggta
    62761 gaaagctgcg gcgtgatggt gtgacgtaca tcttccacgc gggcaaatgc agaaatcacc
    62821 agcgacagcg gcgacgtcat ttcgcgctct tcgttacctt cctgccagcg ggttttcatc
    62881 gacatagagt ctttacccac cgggatcgtc aggcccagcg ccggacaaag ctcttcgccc
    62941 acggctttaa cggcttcata caggcccgca tcttcaccag ggtggcctgc cgccgccatc
    63001 cagttggcgg aaagtttaat gcgtttgata tcgccaatct gtgttgcggc aatattggtt
    63061 aacgcttcac cgaccgccag acgggcagag gcggcgaaat ccagcagcgc aaccggcgca
    63121 cgctcgccaa tcgccatcgc ttcaccgtag tagctgtcga ggctggcggt agtgaccgcg
    63181 cagttagcga ccggcacctg ccacggcccc accatctgat cgcgcgccac catgccggtt
    63241 acgctgcggt caccgatggt caccaggaaa gttttctccg ccacggtcgg cagatgcagt
    63301 acgcgtttca ccgcgtctgc aatggtgatc ccttcacgga ccagcgcatc gcccttcgct
    63361 ttcagcgttt gtacatcgcg ggtcatcttc ggcgttttac caagcaggac gtccagcggc
    63421 agatcgatcg gctgattatc aaaatgacga tcgtgcagag aaagatgcag ttcttcggtc
    63481 gcttcaccaa tcaccgcgta gggcgcgcgt tcacgcttac acagttcgtc aaacagcggt
    63541 aactgatcgg cagcaaccgc cagcacgtag cgttcctggg attcgttaca ccagatttcc
    63601 agcgggctca tgcccggttc gtcgttcaga atatcgcgca gttcgaattt accgccgcgt
    63661 ccgccgtcgc tcaccagttc cggcatcgcg ttagaaagac cgcctgcgcc gacgtcgtgg
    63721 ataaacagaa ttgggttagc atcaccaagc tgccagcaac ggtcgatcac ttcctggcag
    63781 cgacgctcca tctccgggtt gtcgcgctgt acggaagcaa agtcgaggtc ggcatcagac
    63841 tgacctgacg ccatggaaga cgccgctccg ccgccaagac cgatgttcat cgccgggccg
    63901 ccgagaacga ccagtttcgc accaacgttg atctcgcctt tttgtacgtg atcggcgcga
    63961 atgttgccga tcccgcccgc cagcatgatc ggtttgtgat aaccgcgcag ctcttcgccg
    64021 ttgtggctgt tcactttttc ttcataagta cggaagtagc cattcagtgc cggacgacca
    64081 aattcgttgt taaacgccgc gccgcccagc gggccttcgg tcatgatgtc cagcgcggtg
    64141 acaatgcgct caggcttacc gaaatcttct tcccacggct gttcgaagcc aggaattcgc
    64201 aggttggaaa cggagaaacc aaccaaaccc gctttcggct tcgcgccgcg cccggtggca
    64261 ccttcatcgc ggatttcacc gccggaaccg gtcgccgcgc ccggccacgg agaaatcgcc
    64321 gtcgggtggt tgtgagtttc gactttcatc agaatatgcg ccggttcctg atggaaatca
    64381 tagcggcccg tttcgtggtc agcaaagtag cggcccactt cagaaccttc cattacggcg
    64441 gcgttatctt tataagcaga gagaacgtga tctggcgtgg tttcgaaagt atttttgatc
    64501 atcctgaaca gcgatttcgg ctgttgttca ccgtcgataa tccagtcggc gttaaaaatt
    64561 ttgtggcggc agtgctcaga gttcgcctga gcgaacatat acagctcgat gtcgttcggg
    64621 ttacgaccaa gctttgtgaa agcatcctgc agatagtcaa tttcatcttc cgccagagcc
    64681 aggccaagac gcaggttagc gtcgatcagc gcctgacggc cctgccccag caaatcaacg
    64741 cttgtgaccg gagtcggttg atggtgggca aacaactgct ctgcatcatc taaagcaaaa
    64801 aagaccgttt ccatcatgcg gtcgtgcagt tcagcggtaa cctgctgcca ttgttcattg
    64861 gtcagcgtac cggcttctat atagtaagca acgccgcgct caaggcggtt tacctgttgt
    64921 agcccgcagt tatgggcaat atcggtcgct ttcgaagacc agggagagat ggtgccagga
    64981 cgcggggtca ccagcaggag tttgccttgc ggggcgtggc tggcgagtgc cgggccatat
    65041 ttcagcaggc gttcaagttg tgcgtgctca tcatcgttta acggcgcatt gaggtcagca
    65101 aaatggacat actcggcgta aatattgtga accgggagcc tggcagcctg aaaacgtgcc
    65161 agcagtttgt tgattcggaa tgccgacagt gcaggcgaac cacgcagaat ttccatcata
    65221 agtctctcgt cttctaagct ttcggtgtac ccaagggggg gaaacgggcg tcattataaa
    65281 gaatctgatg cgctgacgaa accgtttgcg tggaaataaa atcaccatcg tgaattagca
    65341 acgcgtgccg ccaatggctg taataagttg ccatctggcg caggtttacg caaaatgccg
    65401 ctcatttatg agtaaacctt tcactattat tacgtttttt caagctgg
//
LOCUS       DAPGWD010000009        63835 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPGWD010000009 DAPGWD010000000
VERSION     DAPGWD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.009"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            20..736
                     /locus_tag="RHX88_000522"
     CDS             20..736
                     /locus_tag="RHX88_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2229256.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            766..1266
                     /locus_tag="RHX88_000523"
     CDS             766..1266
                     /locus_tag="RHX88_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2229257.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1358..4042
                     /locus_tag="RHX88_000524"
     CDS             1358..4042
                     /locus_tag="RHX88_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HDV2229258.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4039..5127
                     /locus_tag="RHX88_000525"
     CDS             4039..5127
                     /locus_tag="RHX88_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HDV2229259.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5215..6513)
                     /gene="exuT"
                     /locus_tag="RHX88_000526"
     CDS             complement(5215..6513)
                     /gene="exuT"
                     /locus_tag="RHX88_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDV2229260.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6996..8408
                     /gene="uxaC"
                     /locus_tag="RHX88_000527"
     CDS             6996..8408
                     /gene="uxaC"
                     /locus_tag="RHX88_000527"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDV2229261.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8423..9910
                     /gene="uxaA"
                     /locus_tag="RHX88_000528"
     CDS             8423..9910
                     /gene="uxaA"
                     /locus_tag="RHX88_000528"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDV2229262.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9993..10544
                     /locus_tag="RHX88_000529"
     CDS             9993..10544
                     /locus_tag="RHX88_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDV2229263.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10549..11793)
                     /gene="sstT"
                     /locus_tag="RHX88_000530"
     CDS             complement(10549..11793)
                     /gene="sstT"
                     /locus_tag="RHX88_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDV2229264.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12192..13157)
                     /gene="alx"
                     /locus_tag="RHX88_000531"
     CDS             complement(12192..13157)
                     /gene="alx"
                     /locus_tag="RHX88_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDV2229265.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13440..14426)
                     /locus_tag="RHX88_000532"
     CDS             complement(13440..14426)
                     /locus_tag="RHX88_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV2229266.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14505..15188)
                     /locus_tag="RHX88_000533"
     CDS             complement(14505..15188)
                     /locus_tag="RHX88_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDV2229267.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15265..15768)
                     /locus_tag="RHX88_000534"
     CDS             complement(15265..15768)
                     /locus_tag="RHX88_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDV2229268.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15853..16989
                     /gene="rlmG"
                     /locus_tag="RHX88_000535"
     CDS             15853..16989
                     /gene="rlmG"
                     /locus_tag="RHX88_000535"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDV2229269.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            17180..17263
                     /locus_tag="RHX88_000536"
                     /pseudo
     CDS             17180..17263
                     /locus_tag="RHX88_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17260..17676
                     /gene="higA"
                     /locus_tag="RHX88_000537"
     CDS             17260..17676
                     /gene="higA"
                     /locus_tag="RHX88_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HDV2229270.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17721..19739)
                     /gene="fadH"
                     /locus_tag="RHX88_000538"
     CDS             complement(17721..19739)
                     /gene="fadH"
                     /locus_tag="RHX88_000538"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDV2229271.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20065..22416)
                     /gene="ygjK"
                     /locus_tag="RHX88_000539"
     CDS             complement(20065..22416)
                     /gene="ygjK"
                     /locus_tag="RHX88_000539"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDV2229272.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22433..23503)
                     /gene="ygjJ"
                     /locus_tag="RHX88_000540"
     CDS             complement(22433..23503)
                     /gene="ygjJ"
                     /locus_tag="RHX88_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDV2229273.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23637..25070)
                     /locus_tag="RHX88_000541"
     CDS             complement(23637..25070)
                     /locus_tag="RHX88_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2229274.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDSMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25133..25582)
                     /locus_tag="RHX88_000542"
     CDS             complement(25133..25582)
                     /locus_tag="RHX88_000542"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDV2229275.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25579..28671)
                     /gene="ebgA"
                     /locus_tag="RHX88_000543"
     CDS             complement(25579..28671)
                     /gene="ebgA"
                     /locus_tag="RHX88_000543"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDV2229276.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSNLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28855..29838)
                     /gene="ebgR"
                     /locus_tag="RHX88_000544"
     CDS             complement(28855..29838)
                     /gene="ebgR"
                     /locus_tag="RHX88_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDV2229277.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30057..30389
                     /locus_tag="RHX88_000545"
     CDS             30057..30389
                     /locus_tag="RHX88_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDV2229278.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30431..31810)
                     /gene="ygjG"
                     /locus_tag="RHX88_000546"
     CDS             complement(30431..31810)
                     /gene="ygjG"
                     /locus_tag="RHX88_000546"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDV2229279.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32228..33748
                     /gene="aer"
                     /locus_tag="RHX88_000547"
     CDS             32228..33748
                     /gene="aer"
                     /locus_tag="RHX88_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDV2229280.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33902..34525)
                     /gene="nfeR"
                     /locus_tag="RHX88_000548"
                     /gene_synonym="yqjI"
     CDS             complement(33902..34525)
                     /gene="nfeR"
                     /locus_tag="RHX88_000548"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDV2229281.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34802..35566
                     /gene="nfeF"
                     /locus_tag="RHX88_000549"
     CDS             34802..35566
                     /gene="nfeF"
                     /locus_tag="RHX88_000549"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDV2229282.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35620..35695)
                     /locus_tag="RHX88_000550"
     tRNA            complement(35620..35695)
                     /locus_tag="RHX88_000550"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35660..35662),aa:Ile,seq:cat)
     gene            35820..36326
                     /gene="mug"
                     /locus_tag="RHX88_000551"
     CDS             35820..36326
                     /gene="mug"
                     /locus_tag="RHX88_000551"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDV2229283.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(36405..38246)
                     /gene="rpoD"
                     /locus_tag="RHX88_000552"
     CDS             complement(36405..38246)
                     /gene="rpoD"
                     /locus_tag="RHX88_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDV2229284.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(38441..40186)
                     /gene="dnaG"
                     /locus_tag="RHX88_000553"
     CDS             complement(38441..40186)
                     /gene="dnaG"
                     /locus_tag="RHX88_000553"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV2229285.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(40297..40512)
                     /gene="rpsU"
                     /locus_tag="RHX88_000554"
     CDS             complement(40297..40512)
                     /gene="rpsU"
                     /locus_tag="RHX88_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDV2229286.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40749..41762
                     /gene="tsaD"
                     /locus_tag="RHX88_000555"
     CDS             40749..41762
                     /gene="tsaD"
                     /locus_tag="RHX88_000555"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDV2229287.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41805..43268)
                     /gene="ttdT"
                     /locus_tag="RHX88_000556"
     CDS             complement(41805..43268)
                     /gene="ttdT"
                     /locus_tag="RHX88_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDV2229288.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(43317..43922)
                     /gene="ttdB"
                     /locus_tag="RHX88_000557"
     CDS             complement(43317..43922)
                     /gene="ttdB"
                     /locus_tag="RHX88_000557"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDV2229289.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(43919..44830)
                     /gene="ttdA"
                     /locus_tag="RHX88_000558"
     CDS             complement(43919..44830)
                     /gene="ttdA"
                     /locus_tag="RHX88_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDV2229290.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            45037..45969
                     /gene="ttdR"
                     /locus_tag="RHX88_000559"
                     /gene_synonym="dan"
     CDS             45037..45969
                     /gene="ttdR"
                     /locus_tag="RHX88_000559"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDV2229291.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(45982..46599)
                     /gene="plsY"
                     /locus_tag="RHX88_000560"
     CDS             complement(45982..46599)
                     /gene="plsY"
                     /locus_tag="RHX88_000560"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDV2229292.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46704..47072
                     /gene="folB"
                     /locus_tag="RHX88_000561"
     CDS             46704..47072
                     /gene="folB"
                     /locus_tag="RHX88_000561"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDV2229293.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            47162..47983
                     /gene="bacA"
                     /locus_tag="RHX88_000562"
     CDS             47162..47983
                     /gene="bacA"
                     /locus_tag="RHX88_000562"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV2229294.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(48146..49384)
                     /gene="cca"
                     /locus_tag="RHX88_000563"
     CDS             complement(48146..49384)
                     /gene="cca"
                     /locus_tag="RHX88_000563"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDV2229295.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(49448..50068)
                     /locus_tag="RHX88_000564"
     CDS             complement(49448..50068)
                     /locus_tag="RHX88_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDV2229296.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLLPTRKRKDRWMN"
     gene            50310..51611
                     /gene="ygiF"
                     /locus_tag="RHX88_000565"
     CDS             50310..51611
                     /gene="ygiF"
                     /locus_tag="RHX88_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDV2229297.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51634..54474
                     /gene="glnE"
                     /locus_tag="RHX88_000566"
     CDS             51634..54474
                     /gene="glnE"
                     /locus_tag="RHX88_000566"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDV2229298.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            54522..55955
                     /gene="hldE"
                     /locus_tag="RHX88_000567"
     CDS             54522..55955
                     /gene="hldE"
                     /locus_tag="RHX88_000567"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDV2229299.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(56075..56134)
                     /gene="ibsE"
                     /locus_tag="RHX88_000568"
     CDS             complement(56075..56134)
                     /gene="ibsE"
                     /locus_tag="RHX88_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDV2229300.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(56451..56510)
                     /locus_tag="RHX88_000569"
     CDS             complement(56451..56510)
                     /locus_tag="RHX88_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV2229301.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            complement(56749..58410)
                     /locus_tag="RHX88_000570"
     CDS             complement(56749..58410)
                     /locus_tag="RHX88_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDV2229302.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            complement(58437..59066)
                     /locus_tag="RHX88_000571"
     CDS             complement(58437..59066)
                     /locus_tag="RHX88_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDV2229303.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            59335..59535
                     /gene="glgS"
                     /locus_tag="RHX88_000572"
     CDS             59335..59535
                     /gene="glgS"
                     /locus_tag="RHX88_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDV2229304.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(59578..60642)
                     /locus_tag="RHX88_000573"
     CDS             complement(59578..60642)
                     /locus_tag="RHX88_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2229305.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(60644..61393)
                     /locus_tag="RHX88_000574"
     CDS             complement(60644..61393)
                     /locus_tag="RHX88_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2229306.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            complement(61409..>63835)
                     /locus_tag="RHX88_000575"
     CDS             complement(61409..>63835)
                     /locus_tag="RHX88_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2229307.1"
                     /translation="GCASAYAVEFNKDLIEAEDRENVNLSQFETDGQLPVGKYSLNAL
                     INNKRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEVAQQNLIDGCYPIEKEK
                     QITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASHYAPHQG
                     DNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAK
                     LTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNR
                     IIYQENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYT
                     TAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVGFNLNS
                     LGSLSFDVTRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNE
                     YINDTNHYTNYQNEKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNYSFSLSRD
                     FDIGPLKNVSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTA
                     SWYDSSDRNNSWSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLN
                     ASWNGSFTATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVS
                     SYITTSLSVDTRNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSH
                     PPLGISVKDKTSHKELGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTG
                     TVILPCISQN"
BASE COUNT        15528 a        16006 c        16723 g        15578 t
ORIGIN      
        1 attgtaagat caaatttata tggtggcgaa aattaatctc ttatcacggc ttacgccgct
       61 tttattcgtg ttcgctcctt ttttggcaca gtcaaatatg actgtttatc ctatggctgt
      121 ttccattaat agtcagggtg aaggcaacgt tcgcgtaata tctaaaagta atgaagttca
      181 gtacatcaag gcgacagtat tccgtatcga taatccatcg acgcctcagg aaaatgaagt
      241 tgagattaaa tccggtgatg caaatcatct ggttgttatg ccacctaaat tcgctttacc
      301 agccggtagt agcaaaaccg tacgttttgt tgcgatggaa ccagagcaaa aagagaaaaa
      361 ttatcgcgtt aaatttgaag cggttcccag tattgatgac gttgccacag ataaaaaaga
      421 tctctctatg cagttaacag ttaacttaat ttgggggatt gttgttagtg ttccacctca
      481 gcaacctatt gctaagttag aagtaaatgc tgcccaaaaa ttagttaatg cagggaatca
      541 acgcttaaag attttaacga tcgcttattg taaaaataat agcaaagaaa attgtaagat
      601 acagaccgta aataaaaaca tcttccctgg tcaggaaaga aatcttgaaa gcatatctgg
      661 ctacgacaaa atcgttgtca aatataataa ctggatcacc aaagataatg gcgagtttga
      721 actggcggtc cattaattat cattgtaata aacgaaggaa tagtaatgaa aaaggtattt
      781 gcaaaatctc ttctggtcgc agcgatgttt tctgttgcag gctccgcatt ggcagtacaa
      841 aaggatatta ctgtaacggc caacgttgat gccgctctgg atatgacaca gaccgataac
      901 accgcgttgc cgaaagcagt agaaatgcaa tatctgccgg ggcagggtct gcaatcttac
      961 cagttgatga ccaaaatctg gtctaacgac gttaccaaag atgtaaaaat gcagttagtt
     1021 tccccggcgc aactggttca aagcctggat gctagcaaga ttgttccgct gacagtaacc
     1081 tggggcggtg aagaaattaa agctgatgcg gcaaccactt ttaccgcaac caaaatcttt
     1141 gcttccgatg cgttaactaa cggttctctg gctaaaaacc tgatgtttgc tcaaaccacc
     1201 aaaggtgttc tggagacagg catctaccgt ggtgtagtaa gcatttatct gtctcaggat
     1261 atctaatcca gataacagaa ttaacacgta acaaagggag gggtagccct cccttatttt
     1321 aactgtttta gtatttgtta tggatttcag gattgccatg gataaaaaat tactggctct
     1381 tttgatcctg gcgagtctca gcccggcaga ggcgacatta accaaaattc ccgcagggtt
     1441 tgaggttatt gctcagggac agcaggagta tatcgaggtt tatttttcag ggaaaagtct
     1501 cggtaaatat tatgcaatgg ttaatcttga taccgtaaca tttcttgatc cagcaagtct
     1561 atataacaag ctggaactgg atgtagacga tcagaaaatc gcgcatatag tgaaagaaaa
     1621 attatcgcag ccgctagctc gccacggtga attggcttgc ggttatgtac gtactgactc
     1681 agggtgtggt tttctgaata ccgatacgct ggaaataatc tataatgatg aagaaagttc
     1741 ggcaacgttg tttattaatc cgcaatggaa ttcagctttc gatgcgaagt cattatattt
     1801 aaatccagac aaaaatactg ttaatgcttt tatacatcag caagacatca atgttctggc
     1861 acaggatgat taccaatcgt tgtctattca gggaaacggt gcgctgggaa taacagaaaa
     1921 tagttatatt ggtgcacact ggaatttcaa cggttatgat gcagatgatg tcagtgacag
     1981 taatgctgac gtcagcgatc tctattatcg ttatgatttt ttacgtcgtt attatgtgca
     2041 ggcgggtcgc atggacaacc gcacactatt taatgcacaa ggcgggaact ttacctttaa
     2101 ctttctgcca ctcggtgcaa ttgatgggat gcgtatcggg tcgacactga gctatttaaa
     2161 ccaggcgcaa agccagcaag gaaccccagt aatggttctg ctttcgcgca attctcgtgt
     2221 tgacgcttat cgtaatgagc aacttttggg atcattttat ctcaatagtg gttcgcaatt
     2281 tattgatacc agttcctttc cgcctggcag ctacagcgtg gcattaaaag tctacgaaaa
     2341 taaccaactc acccgcaccg agcttgtgcc gtttaccaaa accggcggtc tgactgacgg
     2401 aaatgcgcaa tggttcttac aggcaggtaa aactacatca caggtttctg atgatgaaag
     2461 ttcagcttat cagctaggag tacgcctgcc attacatccg caatatgagc tctacgcagg
     2521 gctggcgaat gccgatgatg tgagtgcttt cgagttaggt aataactgga cggcagattt
     2581 aggcggggtg gggaatcttg caatcagcgc cagcgtgttc cgtaacgatg acggcggcaa
     2641 aggtgatatg caacaggcca actggagtaa tccgggatgg ccgacgttgg gcttttatcg
     2701 gaccaactct gacggcgatg cttgtacaac cgacagcaga gagagctata acgccttaag
     2761 ctgttatgaa agtatttccg cgacggtttc acagaatttt gtcggctgga atatgatgct
     2821 gggttatacc cgcacacaaa ataacactga tgatagtttg cgttgggata aacagcagag
     2881 ctttgaaaat aactatcttc gccagacaac agcgcaaagt atctctgaaa ctgtacaact
     2941 tagcgcttcc cgcgcttttg tgatgcgtga ctggattttg agtacgtcag tcggtgtttt
     3001 ccatcgtaat gacaacggtg gcgataacga cgacaacggc ttgtacttat cgttttcgtt
     3061 atctgacacg ccaacgatgg atagcaataa caacagccat tcaaccaatg tgtctacgga
     3121 ttatcgttat agcgaacagg atggcgatca aacgtcatgg cagttatcgc atacctttta
     3181 taacgattca ttcagccata aagaacttgg cgtaaccgtc ggaggcctga acaccgatac
     3241 cataaacagc gcggttaacg ggcgttggga tgggcaatac ggaaatgtct acgctaccgt
     3301 atctgatagt tatgaccgta agaatcatga tcatctttcg gcctttacgg gcacttatag
     3361 ctctacgctg gctgtcagtc gctatggcgt taatttggga gccagtggta cagacgattt
     3421 gctgggtgcg gtactggtgg atgtgaaagg cttctctgaa caggatgaag agagtcagga
     3481 tctgcaactc gaagcgcggg tggcaggcag ccgaactttg cagcttggtc aaagtgacag
     3541 tgtgttgttc ccttatcctg gatttcagtc tggttttgtt gaggttaacg acagtagcca
     3601 gggcaatcag caggggacaa caaacatcat taacggtgca gggaatcgtg aattaatgtt
     3661 gttgcctggc aagctgcgct atcgcgaagt gtctgccagc tttaactaca actatatcgg
     3721 tcgtttatta ctgccggcag cggtgaaaaa attcccgatt gtggggctga acagcgccat
     3781 gttactggta gctgaagatg gcggatttac acttgaaatt aacggcagcg aaaaagagct
     3841 ctatctgctt tccgggcagc aattccttaa gtgtccacta agtgttgtaa agaaacgcgc
     3901 cagcattcgt tacagcggag atgttacttg tagtgtggtg acttattcac aattaccgga
     3961 gtcgattcag gttcaggcac agttgaaaca gcctaaatta cgtggaaacg ttcagacggc
     4021 gcaaagggag gttgcaccat gagaaatcga ttgattgcgg cgatattggg cttgtttggc
     4081 acgctcactg gcgttcaggc agctcctgac gtgaccagtg aaattacgta tgatttggca
     4141 tctggtagag cggattatta cttctggaag gatgaagcgt cggcaggaaa taatggatat
     4201 atgtggtatg aatgttcgta tcctgacctc caacaaacct gtacagctaa tggaaatata
     4261 tcgacagtac aaatctattt aactgaacaa cgcagtggga tgcgttggcc ggtaaaactc
     4321 aaaggattta aaacagccat tgtaagtagc gatgaagcgc cgccaggatg caaggggggc
     4381 aaagggcttc agacgaatct taaggattct aatagatctt catgtacaga agatggtcaa
     4441 cattattata tatacgatac aaagtttctt acgctctacc ttgagcagac agagatgaag
     4501 aatttgccga ttggtggcgt ctggaagggg aaagttaaat tacattcgaa cagcccggcc
     4561 caggactatt tcgcaaatat taccctgaat acgctcgacc ccaaccatat tgacgtgttc
     4621 ttcccggagt tcgcccacgc cacgccaagg gtgcagttag acttgcatcc aacaggaagc
     4681 gttaacggca gcaactacgc gcaagatctg accatgttgg acatgtgcct gtacgatggt
     4741 tttaacggta atgccatcag ttatgaaatc atgctcaaag atgaagggcg accagctgca
     4801 gggcgcagag acggttactt ctctatctat cgtcagggag ggaccaccac cgacgaggga
     4861 gaacgcattg attaccgggt caaaatgtac aacccggaaa ccggtgggca aattgatgtg
     4921 cgcaataatg aaaatatggt ctggaacagc attaacctga aacgtgtgcg tccggtggta
     4981 ctgcccggta ttcgctatgc cgtgatgtgt gtgccgacac ccttgactct ggcagtcgat
     5041 aaattcagcg tgatggataa acaggccggg tactacatgg gcaaattgtc agtaatcttt
     5101 acgccttcct tgccaaccat caattaaccc tgcaaagcgg acaaacgctt ttttctatat
     5161 aaacgacgaa gccgtctgat aaagcggctt cgtcattaca acttaagtta aacgttaatg
     5221 ttgcggtgcg ggatcgttat gtgtttcctg cgccacctca atcgccggtt tgttctgcaa
     5281 cacggtccag ataaccagcg cacctaacag gtcaaatact gccagaactg cgaacagcgg
     5341 gctgaagccg atggtgtcag ccagtgcacc gacaaccagc gcaaacagcg tacttgccag
     5401 ccatgcggac atcccggtta aaccgttcgc cgtcgccact tcgttacgac cgaacacgtc
     5461 agaagagagc gtaatcagcg cgccagacag tgcctggtgg gcaaaaccac cgatacacag
     5521 cagcataatt gcgacatacg ggttggtgaa cagaccgatc atacccgggc caatcatcag
     5581 cacagcaccc agcgttacga ccatcttacg ggaaacgatc aggttcacac caaaccaacg
     5641 ctggaacagc ggcggcaggt aaccaccgag gatacaaccg aggtcagcaa acagcatcgg
     5701 catccaggcg aacatcgcga tctctttcag gttaaagccg taaactttaa acatgaacag
     5761 cgggatccac gcgttaaaag taccccaggc cggttctgcc aggaaacgcg gcagtgcgat
     5821 accccagaac tgacggttac gcaggatctg accaacggac attttcttcg ccgtgctcac
     5881 ctggtgctgg gcttcctgac cattaataat atagtcgcgt tcttcatcgg tcagatgctt
     5941 ctggtcgcgc ggatgtttat agaaaatcag ccatgccatc gcccagataa agctcaatgc
     6001 accggagatg ataaatgcca tctgccagct gtgcattacg attgcccata ccaccagcgg
     6061 cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaag taacctaccg cgatggaacg
     6121 ctctttcgcc gggaaccatt cggagctggc tttcagaccc gccgggatca tcgctgcttc
     6181 cgcggcaccg actgcaccac gagcaatcgc caggccaccc cagctaccag ccagcgcggt
     6241 tgcaccacag aacacggccc acagcacagc aaacattgca taaccgattt tcgtacccag
     6301 cacatccagt acatagcccg ctaccggttg catgaccgta taagcagcag aataggctgc
     6361 gatgatatag gaatactgtt gggtggagat gtttaactct tccatcagag ttggcgcagc
     6421 tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg ccaagcgtca ccagtgcgat
     6481 catataccaa cgtaaccctt taattttacg catgtaaaac ctcatcgttt tgttatatcc
     6541 gcaggaagcg cggccatcat cccgccagag gctgaagatg ttagttacga ccgggtaacc
     6601 gaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt tcgtgctaat tcggcttccg
     6661 taccggttaa cccgattcat ctaacaaatg aatgaatcgg caatttgcat tccgtcgact
     6721 tatgatttgc gacggcagaa agataacttg tcatacaact ttaaaaggtg agagccatca
     6781 caaatgtgcg aatatttgta gggacattac ctgacgacag cgaggccagt actggcgcgg
     6841 cctgcagaga gatttaccac tttgagagta atttttttaa ctacgtttat tgatctaact
     6901 cacgaaaata tcttcggact ctggaaattg gtgtgataac tttgtcagca tcgcaccata
     6961 agcaagctag ctcactcgtt gagaggaaga cgaaaatgac tccgtttatg actgaagatt
     7021 tcctgttaga taccgaattt gcccgccgtc tgtatcacga ctacgcaaaa gaccagccga
     7081 ttttcgatta ccattgccat ttgccgccgc agcagattgc ggaagactat cgttttaaaa
     7141 acctgtatga catctggctg aaaggcgatc actacaaatg gcgcgctatg cgtaccaacg
     7201 gtgtggccga gcgtctgtgt accggtgatg cgtctgaccg tgaaaaattt gacgcctggg
     7261 cggcgactgt tccgcacact atcggcaacc cgttatacca ctggacgcac ctcgaactgc
     7321 gtcgtccgtt tggtatcact ggcaaattgc tttctccgtc aactgccgat gaaatctgga
     7381 acgaatgtaa cgaactgctg gcgcaggata acttctccgc gcgcggcatc atgcagcaga
     7441 tgaacgtgaa aatggtcggc accaccgatg acccgatcga ttctctggag catcacgcag
     7501 agatcgccaa agacggctct ttcaccatca aagtgctgcc gagctggcgt ccggacaaag
     7561 ccttcaacat cgaacaggcg acctttaacg actacatggc gaagctgggc gaagtttccg
     7621 ataccgacat tcgccgcttt gctgacctgc aaactgcctt gaccaaacgt ctggatcact
     7681 tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga cgtggtgatg tttgctgaag
     7741 cgaacgaagc ggaactggac agcattctgg cgcgccgtct ggctggcgaa cccctgagcg
     7801 agcacgaagt ggcacagttc aaaactgcgg tactggtgtt ccttggtgcc gaatatgcac
     7861 gtcgcggctg ggtacagcag taccacattg gcgcgctgcg taataacaac ctgcgtcagt
     7921 tcaaactgct ggggccggat gtaggctttg actccatcaa cgaccgtccg atggcggaag
     7981 agttgtctaa gctgctgagc aagcagaacg aagaaaacct gctgccgaaa accattctct
     8041 actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat gatcggtaac ttccagggcg
     8101 aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg gtttaacgat cagaaagacg
     8161 gtatggaacg tcagatgacc caactggcgc agctcggttt gctgagccgc tttgtcggta
     8221 tgctgactga cagccgtagc ttcctgtcat acacccgtca cgaatacttc cgccgcattc
     8281 tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc accggcggac atcaacctgc
     8341 tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg tgactacttc gccattgaac
     8401 tgaactaagg tctggggttg atatgcaata catcaagatc catgcgctgg ataacgtcgc
     8461 ggtcgcttta gcggatttgg ctgaaggcac agaagtcagt gtcgataacc agactgttac
     8521 gctgcgccag gatgttgctc gtggacataa atttgcgtta acggatatcg caaaaggggc
     8581 caatgtcatc aaatatggcc tgccgattgg ttatgcattg gcggatattg cggcggggga
     8641 acacgtccac gcccacaata cgcgcacgaa tctgagcgat ctggatcagt atcgctatca
     8701 acctgatttt caggatctgc ctgcgcaagc ggcagatcgt gaagtgcaga tctatcgtcg
     8761 cgctaacggc gatgtcgggg tgcgtaacga gctgtggatc ctgccaaccg tgggctgtgt
     8821 caacggcatc gcgcggcaaa tccagaaccg tttcctgaaa gagaccaaca acgccgaagg
     8881 taccgacggc gtgttcctct tcagccacac ctacggctgc tcacaactgg gcgacgatca
     8941 catcaacacc cgcaccatgc tgcaaaacat ggtgcgccac ccgaacgcgg gcgcagtgct
     9001 ggtgattggt ctgggctgtg aaaacaacca ggttgccgca ttccgtgaaa cgttgggcga
     9061 tatcgatcct gaacgcgttc atttcatgat ctgccaacag caggatgatg agatcgaagc
     9121 cggaatcgag catttgcatc agctgtataa cgtgatgcgc aacgataaac gcgagccagg
     9181 caaactcagc gaactgaagt ttggtctgga gtgcggtggt tctgacggtc tttctggtat
     9241 tactgctaac ccgatgctgg ggcgtttctc tgactacgtg attgctaacg gcggtactac
     9301 cgtactgacc gaagtgccgg agatgtttgg cgcagagcag ttgctgatgg accattgccg
     9361 cgacgaagca acgtttgaaa aactggtcac catggtcaac gacttcaaac agtactttat
     9421 tgcccatgac cagccgatct atgaaaaccc atcgccgggg aacaaagcgg gcggtatcac
     9481 cacgctggaa gataaatccc ttggctgtac ccagaaagcg ggttccagcg tcgtggttga
     9541 cgtgctgcgt tacggcgagc gtctgaaaac gccggggctg aacttgttaa gtgcgccggg
     9601 taacgatgcc gtagcgacca gcgccctggc gggtgcgggc tgccatatgg tgctgttcag
     9661 tactggtcgt ggcacgccat acggcggctt tgtaccgacg gtgaaaatcg ctaccaacag
     9721 tgaactggcg gcgaagaaaa aacactggat cgactttgac gcgggtcagc tgatccacgg
     9781 taaagcgatg ccgcagttgc tggaagaatt tatcgacacc atcgttgagt ttgccaacgg
     9841 taaacaaacc tgcaacgagc gtaacgactt ccgcgaactg gcgatcttca aaagcggcgt
     9901 aacgctataa agtcgaaatg agttgttaat aaaaaacggc gtttcataat gtgaagcgcc
     9961 gtttgctttt ccatacctgc taaggattta tcatgaccgc gtattggctg gcccagggcg
    10021 tgggtgtcat cgcctttctg attggtatca caacattttt caatcgtgac gaacgtcgct
    10081 tcaaaaagca gctttcggtc tatagcgccg ttattggcgt acattttttt cttctgggca
    10141 cctatcccgc tggtgccagt gccatcctta atgccattcg tacattgatt accttacgca
    10201 cgcgcagctt atgggtaatg gcgattttta ttgtgctgac tggcggaatt ggcctcgcga
    10261 agttccatca tcctgtcgaa ctattgccgg ttatcggtac gattgtcagt acctgggcgc
    10321 tgttccgctg taaagggctc accatgcgct gcgtgatgtg gttttcaacg tgttgctggg
    10381 tgattcacaa cttctgggcg gggtcgatag gcggcacgat gattgagggt agttttctgc
    10441 ttatgaatgg cctgaatatc attcgtttct ggcggatgca gaaaagggga attgatccgt
    10501 ttaaagtaga gaaaacccct cccgccgtag acgaaagggg ttaaacaatt aattacgcag
    10561 ggcgctattt gccagacgat cgtcttccgc ctggcaagct gccgcagtga acagcacgtc
    10621 agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat acgccgatga taaagccgac
    10681 ggcaaccacc tgcatggcga tatcgttcga aataccgaac atattacagg ccagcgggat
    10741 cagcagcaga gaaccccccg ccacgccgga tgcgccacag gcacacagag aagccaccac
    10801 gctcaacagc agcgccgtgg gcagatcgac cgggataccc agcgtattaa ccgcagccag
    10861 cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg atggtggcac ccagcggaat
    10921 agaaacggaa taggtatcgc gatccagatt cagcttttca cacagtgcca tattcaccgg
    10981 aatgttcgcc gcagagctgc gggtgaagaa ggcatacacg ccgctttcgc gcaggcacag
    11041 cagcaccagc gggaacgggt tacgacgaat tttccaccac accagcaatg ggttaaccac
    11101 cagcgccacc agtaacatac agccaaccag cacgaccagc agttgcgcgt agccccacag
    11161 tgtggagaaa ccggtggttg ccagggtaga agaaaccaga ccaaaaatac caatcggtgc
    11221 gaagcgaatg accagtttca ccataaaggt gacggcattc gacatatcgt tgaccaggtt
    11281 tttggtggtc tcgttaccgt gacgcagtgc gaaaccgagg ccaattgccc acaccaggat
    11341 cccgatgtag ttacctttca gcagcgcgtc gatggggttg gaaaccatgc tcattaccag
    11401 cccgcgcatc acttctacaa tgcctgacgg tggcgaaata tcacccgcgc tactggataa
    11461 gtgcagggta gaagggaagg cgaagctgaa aacgactgcg gccagagcag cagagaaggt
    11521 acccagcaga tagaggaaca aaatagggcg gatattggtt ttctgcccgt gctggtggtt
    11581 agcaatagat gccatcacca gcatcaacac caggatgggg gcaacggctt tcagtgcgcc
    11641 gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg ggttttgaga tccatgccag
    11701 aagaatcccc agaacaaggc cgaccaggat ttgttttacc aggctgccat gagccagacg
    11761 ccggaatagc cccggtgaac gttgcgtagt catttttcga tcctttcatt gtgttgtcgc
    11821 acatccctgg tgcgttctgt cgacgcatct ttcaggatgt aacaaagtgt ttgcaaggtc
    11881 gagtataagg aaagttacag cttcgggaag cgaaaaatgc tggattttaa agagaggtca
    11941 tattttgcaa cttaatggtt acatttattt gacataaaaa taaagccaac gtgcggcttc
    12001 cagactgatg acaacatcaa aaaaggactc ggtcagtccc ctcgccccgt ttggggagag
    12061 ggttagggtg aggggaacag gccagcatcg gtgcgaaaaa ttaccctcac cccgaccctc
    12121 tccctggaag ggagagggag aaaacggtgc tcatgttaat gacagggtac acggcttagg
    12181 cagattaaaa attatccacc ccgctgctta tcatgccgat aattcaccca ggcgttgata
    12241 ataaacgtca tcaccagaat gccaaacacc acgcccagcg agacggcgat tggaatatgg
    12301 tagaagtcga caatcagcat cttgataccg ataaacacca gaatcaccgc caggccatat
    12361 ttgagcatcg agaaacgctc tgctacgccc gccagcagga aatacatcgc acgcaggccg
    12421 aggattgcaa acaggtttga ggtcagcaca ataaacggat cagtggtcac ggcgaagata
    12481 gccggaatgc tatccacggc aaaaatcacg tcgctcaatt ccaccagaat cagtaccagc
    12541 atcagcggtg tggcgtacag caatccattc ttacgcacaa agaaatgctc gttgtcgatg
    12601 gtgtcggtca tgcgcaaatg accgcgtagc cagcgcacca gcggcttgtc gccaatgcct
    12661 gattcatctt catgggcgag cgccatcttc acgccggtaa acagcaggaa ggcaccgaag
    12721 atatacagga tccagtcgaa ctgtgaaatc agccagctgc ctgtgaagat catgatggta
    12781 cgcagaacaa tcgccccgag cacgccatac accagcacgc ggcgttgtaa tgccgccgga
    12841 acagagaaat agctgaacaa catcagccag acaaagacgt tatcgaccgc cagcgatttc
    12901 tcaatcagat agcccgtgag aaaggccagt gcctgtggat cggcaacggc gcgaccttca
    12961 gtttgcacca gataccacca gaaagcggcg ttaaacagta acgacagcgt cacccagacc
    13021 agcgaccagg ccgcagcctg tttcatggtc atggcatgtg ccccacgacg cccctgcaac
    13081 aacaggtcga tagccagcat aatggcgaca acaacagcga atccgcccca tagcaacggc
    13141 gtgccgacag tattcataga agttccttac acataaaaaa acggccaacg tcggaagacg
    13201 tcagccgctg ctttttatgc atagacctcg ccttccggca aggtctcact tacaacaaaa
    13261 aaggaatacg tctgcgtatt cctttcgggt tgcccggtga ccggatgtgg tttttcacac
    13321 atcgtaatga cgatcgaccg gcaatgaagt tactcccctt tgcaggtaac aaagtatgac
    13381 agaccattca gccagtcaat ggtctgttac atacctttta cactgtttta ctttggcaat
    13441 tatagtttta cgctatctgc cggaaaaatc accccagtct ggcggcggat ctcggtcagc
    13501 agtttggcgg tgatgcgact gaccgccagc cccggatgat ccaccagatg ctcatccacc
    13561 agggtagcga acagctctgc ttcgtagagc atggtattaa tatgctgcgg ctgggtgaga
    13621 tcctgcattt ggctgccacg cggcacgaag cacactttct ggcattcaga cagtttttca
    13681 atcaccagcg atcctgcttc gccctgaatc tcgctcgcca ggacagaatc actgactttt
    13741 gagtgctgca aggtgacgct gaaatcaccg taatccatca ccaccacgcc gtgggcatct
    13801 acaccgcttg ccagcaaact ggcggttgcc tgcacgcttt tcggctcacc aaataacgcc
    13861 accgccgacg ccaggcagta aaagccgata tccataattg aaccgttaga gaatgacgga
    13921 ttaaaggtgt tgggattttc accgtccagg taacgttgat agcgcgagga atactggcaa
    13981 tagttgaaaa agactttacg caatttgccg actttcggca gcgcctggcg cagcaaatga
    14041 aagttcggca ggcaggcggt tttaaatgcc tcaaacagca ccacctgatt ttcccgcgca
    14101 caggcaatgg cggcatccac ttccgccaga ttcgacgcca gtggtttctc gcaaatcaca
    14161 tgaattttat ggctaaggaa aagttgtgtc tgggaaaagt gcagggcatt cgggctggca
    14221 atatacaccg cgtcaatggc atcgctttcc gccatcgctt ccagcgaggt aaacagatgc
    14281 tcgacagaaa aatcattggc gaagtgctga gcctgctcaa ggctgcggga atatacggcg
    14341 gttaacttgt atttaccgct ctcatgggcg gcctcgacga actggcgagt gatccagttc
    14401 gtaccaatca cagcgaaacg tatcataacg cgtggatact ccataaaggg attctgtcgt
    14461 caatgtagca cgcctttttc atcttcgacg tgcgcagata cgcattattc ttttgccgga
    14521 cagccatgct cactaaacgg cccaatcatg accgatggcc cgagatattt cggctggcga
    14581 tggaggattt tggcgtcaat cacggcgctg acacgagcga gtttctcccg cagttgagta
    14641 taaaaaccgt gcttcatatt gaggtcaggc gcgttaaaac cgatagcgtc gatgccgtac
    14701 tgttttgcca gccagatggc gcgttgatta tgaaattctt gcgagataat ggtgatatgg
    14761 ttttcaccaa aaaccttttt ggcacgcact accgaatcca gcgttgagaa tccggcatag
    14821 tcgcagaaga tcactttcgc cggtacgcct ttagcgatca aagcctgctg catacctgag
    14881 gcttcatcgt aatttttacg tccgttatcg ccacttacca gtagccattt cacttttccg
    14941 gcgtggtaca acgctgctgc ggtatcaata cgtcgggtaa agtagcgatt acccggcctg
    15001 gcccccagca ataagcccac gttacgcgcc ggaactgcgt tgacatcgct ccaggtcagc
    15061 tgcttgctgg cgttgaccat aacgcgatcg gcaataaaaa tggtcgctcc tgctataagc
    15121 aacaacaaac agccaatctt aagagtccgg cgagaaaaac aaatacggag aagaaggcgg
    15181 ggctgcatgg tcgttatcct taacgaaagg ggggaggtcg attttaggcg ctaaagaaag
    15241 aaaagataac gcctgacatc attttcagat tttgtcctgc ccaagcgaca actgcgttag
    15301 ccacagccgg gtgtcgaact caagctggtg gtactgcggc tccatatggc agcacaactg
    15361 gtaaaacgct ttgttatgct ctttctcctt caggtgcgcc agttcgtgca ccacgatcat
    15421 acgtaaaaac ggttccggcg cgttgcgaaa cacagtagcg acgcggatct ctgcttttgc
    15481 tttcagcttg ccgccctgca cacgagaaac agcggtatgt aacccgagcg cgttttttag
    15541 cacatggatc ttattgtcat acatcacttt attgatcggc ggggcattgc gcagaaactg
    15601 attttttaga tcctgagtat attgccagag ggctttatcg gtggcgtagt cgtgtgttcc
    15661 cggatagcgt tttgccagca catcgcccag acgctgttcg ttaatcagcg ttcgcacctg
    15721 ggaaagtagc tgctcgggat aaccctgaag ataagtaaga ttgctcataa ccgcccacga
    15781 aaaagagaaa aagggtatac tcacgcaccc ttttcagggg caacgtcgaa attttatcat
    15841 tcaggagggc cgatgagcca cttagacaac ggtttccgtt cactgacact acaacgtttt
    15901 ccggcgacgg atgacgttaa cccgctacag gcgtgggaag cggcggatga atatttgctg
    15961 caacagttgg acgacacaga aatccgcggc ccggtgttga tcctgaatga tgcctttggt
    16021 gcgttaagct gcgcgctggc ggaacataag ccgtacagca ttggcgactc atacatcagt
    16081 gaactggcga cgcgcgagaa tttacgcctc aacgggattg atgagtcgag cgtgaagttt
    16141 ctcgacagca ccgccgacta cccgcaacag ccgggtgtgg tgctgatcaa agtgccgaaa
    16201 acactggcat tgctggaaca gcaactgcgt gcgctgcgca aagtggtcac gtcggataca
    16261 cgtattattg ccggtgccaa agcccgtgac attcacactt ccacgctgga gctgttcgag
    16321 aaagtgctcg gcccgaccac cactacgctg gcatggaaaa aagcgcgcct gattaattgc
    16381 actttcaatg aaccgccgct ggccgatgcg ccgcagaccg ttagctggaa gctggaaggt
    16441 actgactgga ctatccacaa ccatgcgaat gtcttctccc gcaccgggct ggatattggc
    16501 gcacgcttct ttatgcaaca tctgccagag aatctcgaag gtgagattgt cgatctcggc
    16561 tgtggtaacg gcgttattgg cctgacgctg cttgataaaa acccgcaggc gaaagtggtg
    16621 tttgtcgatg aatcgccgat ggcggtcgct tccagccgaa tgaatgttga aaccaacatg
    16681 ccagaggcgt tggatcgctg cgagtttatg atcaacaacg cgctctccgg agtggagcct
    16741 ttccgcttta atgctgtgct ctgcaacccg ccgtttcatc agcaacatgc gctgaccgat
    16801 aacgtcgcct gggagatgtt ccaccacgcc cgccgctgcc tgaaaatcaa cggcgaactg
    16861 tatatcgttg ccaaccgtca cctggattac ttccataaac tgaagaagat tttcggcaac
    16921 tgcaccacca tcgccacgaa taataaattt gtggtgctga aaacagtgaa gctggggcgt
    16981 cgtcggtaag attgggagtt acctgccgga tgcggcgtta acgccttatc cggcctacgt
    17041 tcagcacgtt actcccaggc cggataagac gcggcaagcg tcgcatccgg caatgtctgc
    17101 ccagttctgg aatcgcgctt ccataaaaca aaaaatccct gtcaccatcc tctacattct
    17161 cttgtttagc gttgttcaac gtttatgcta catacgtgaa gttatgacgc ataaagaata
    17221 cgatttcttt accgctgttc atcgtactaa ggggaaaaaa tgattgcgat tgccgacatc
    17281 ttgcaagcag gagaaaagct aactgcggtg gcaccttttc tggcgggtat tcagaacgag
    17341 gaacaatacg cccaggcgct ggaactggta gatcatctgc tgctcaacga tcctgaaaac
    17401 cccttgctgg atctggtgtg tgccaaaata accgcgtggg aagaatcagc gcccgaattt
    17461 gcggaattta atgccatggc tcaagccatg cctggcggta tagccgtgat tcgtaccctt
    17521 atggatcaat atggtttaac cctttccgat ctgccggaaa ttggcagtaa atctatggtg
    17581 tcacgcgttt tgagcgggaa gaggaaatta acgctggaac acgctaaaaa attggcaacg
    17641 cgattcggca tttctcccgc cttgtttatt gattaacgcg acgtgccgga tgcgttgcat
    17701 ccggcgcttc aaataacgat ttaaatctcc agcgccagcc gtgttccctg ggcaattgcc
    17761 cgtcgtgcgt ccagctccat agccacatcg cagccgccaa ttaaatgcac agttttcccg
    17821 ctatcaatca gcggttgcgc cagcgcgcgg tttggctctt gccctgcgca gatcaccaca
    17881 ttgtccactg ctaatacctg cgtttcgccg ttgatcacca catgcagccc gtcatcgtca
    17941 atcttctgat aacttacgcc tgggatcatt ttcacacccc gcgagagcag ggtggtgcga
    18001 tggatccagc cggtggtttt gcctaacccc tgtcccggtt tgctggcttt gcgctggagc
    18061 atcacaattt gccgtgggct acgcgggatc tgcattccct gcgggcttaa gccaccagcc
    18121 tgttgtaggc tgctgtcgat cccccactca ttacagaacc tggcgatatt ctggctggtg
    18181 gattcgcccg gctgacttaa atacatcgcc gtatcaaaac caatcccgcc acaaccgatg
    18241 atggcaacct tgttgcctac cggcgctttg tcgcgcaata catcgagata actcaatacc
    18301 ttcggatgat cgatcccgtc gatgggcgga atgcgcggca cgatcccgct ggcaaggatc
    18361 gtttcatcga acgactgtaa ctgatccgcc gtcacggtgt gattgagttt cagcgtcacc
    18421 cccgtcactt cgatcatccg gcggtaatag cgcagcgttt cgtaaaattc ctctttgccg
    18481 gggatctgtt tggcgatatt aaattgcccg ccgatctcac tgtgggcatc aaacaacgtt
    18541 acctgatgcc cacgcgccgc cgcgttaatg gcaaacgcca gcccagcagg tcccgcaccg
    18601 accaccgcca gatttttttt ctgcacggca ggaaggattg gcattttggt ttcgtggcag
    18661 gcgcgaggat tcaccaggca cgaggtgact ttgccaacga agatctgatc gaggcaggcc
    18721 tgattgcagc caatgcaggt gttgatctca tcggctcgcc ccgattgcgc ttttgacagc
    18781 agttccgcat cagcaagaaa cggtcgtgcc atcgacacca tatcggcatc gccgcgcgag
    18841 agaatgtcgt cggcaacctg cggatcgtta atccggttgg tggtcaccag cggcagcgaa
    18901 acgtggcctt tcagtttgcg cgtgacccaa gtaaatgcgc cgcgcggcac gggcgtggca
    18961 atggtcggaa tacgtgcttc atgccagcca atgccggtgt tgataatggt cgcgcccgcc
    19021 gcttcaatgg cctgcgccag ctctaccgtt tcggcaaaag tcccgccgtc ttcgaccagg
    19081 tcgagcatcg acagtcggta gataataatg aagtcgttgc cgacgcgttc gcgcaccgca
    19141 cgcactactt ctacggcaaa tcgcatccgg ttgcggtaat cgccgcccca ctggtcacta
    19201 cgctgattgg tgcgcagcgt cagaaattcg ttgatcaaat acccttcgga ccccattacc
    19261 tctacaccgt cgtatcctgc ctcccgtgcc aattgcgcgc agtgggcgaa atcgtcgatc
    19321 agttgcagga tctcttcatg ggtgagttcg tgaggaacga aacggttgat gggggcctgc
    19381 aatgcggagg gagcgaccag atgcggttgg tagctgtagc gcccggtatg caaaatttgc
    19441 agggctattt tgccgccttc ctgatgtacc gcttcggtaa tggtgcgatg gtgtgggatc
    19501 tggctggcgt cgttgagcat tgcgccacct tccatgccaa cgcctgttaa atctggtgca
    19561 atgccgccgc tgacaatcag cgccacgccg tgacgggcgc gttcggcata aaacgctgcc
    19621 agccgctcgg caccgtccgg gtattcctcc agcccggtgt gcattgagcc catcaacacg
    19681 cggtttttta acgtggtaaa acctaaatcc agcggggcga acagcgacgg gtagctcata
    19741 aaattgtcca gtatgtaaaa taattgttat gtggtcggat gagttctaat ttagccgtcg
    19801 ccggagaaaa gggaaaaggg gagtgcgggg tttgtgatgg gattcaaaaa gaatacgccc
    19861 ggtggtaacg ccggatgcga cgcttgccgc gttttatccg gcctggggga ttgcacatgt
    19921 agggcggata aggcgttcac gccgcatccg ccagtggcac ggtgcagatg ccggatgcga
    19981 cgcttgtcgc gttttatccg gccaggagga ttgcacatgt agggcggata aggcgtttac
    20041 gccgcatccg ccagcagttg caaattattg cttacggaaa aaatcgttat acaacatata
    20101 caaatgcgcg gcactccagg agaaatttgg tgcgccttgc tgtgcgccag tcagcgggtt
    20161 gtaattctcc tgaattgggc catcggcggt taatcctttg gcgtgacgga agaacgtatc
    20221 cgccagtttc agggcatcat cgcgataacc gtaacgctcc atccctttca gaccaaacca
    20281 gaactgatcc acccatacgc gcccacgcca gtagatatca gcgccaaaag ccggattggt
    20341 taacgccgcc gttcccagcg ggacaaaggt gttgaactct ttaggatcga gcatcacctt
    20401 caccaccgcg tcggcattgg cctgcgttgc cgcaccgtta aacagcggcg accagccttc
    20461 cggcccttta ccgcgctcaa caatcggttt gcccgcgcag ccgttcgcca gtggtttatc
    20521 ttcaatacgt acgtcatagt agaactgggt agtcgggtcg aacatacagg tgttgatgta
    20581 gtccgcgagc tgctgtgcca actggcgata gcgtttggcc tcttctggct taccgaggat
    20641 ggttgccatc tccgccagat aatggttatc gctgtacata tagctggcct gatccaccga
    20701 ctcctgcaat agcgagtagc ccagcaacgt tccgtcctga ctgcggtttt cggcgaattt
    20761 caccgtccag tcgctacgtt tgccgccatt agcgacatat ttatccaact gttctttgtc
    20821 gataaaacca aagacggcgg catcatcacg ccccgattcc cacgacgcag cgacctgtgc
    20881 cggaatttcc agactgtcgt actggccttt ctccaccacg cgggcgtagt tgttcagccc
    20941 agactgcgtc tcttctttgt tgcctttctt caccgtaaac agcatctcgc cgctctcagt
    21001 gttgtgggct ttgtcgcggg tcgcgccata ttccggcacg ccgttgccgt tgtgatcgcg
    21061 gttacgtaac caccagtcgt gataggccac cagtttcggg tacatctctg ccaaccaggc
    21121 tttatcctgg gtgacgttgt acacttccat caccgaccag gcggcaaggc tgggtttggt
    21181 attacgttcg ttccagttgc cgccatcgcc accacgctcg gggctaaggt tccacgctat
    21241 caggtcgggg acaaagccca catcctgcgg acgcacgcta tcgccaggct ggatctgcca
    21301 ggagaagacc gcgcggatat tctctttggc gatgtccgga ttgaaatgcg ccatcgcaaa
    21361 cgcctgcttc caggtatccc acggccaggt ctgattgccg gagaaccagc gcccggtcac
    21421 cgacggtgta acggtgttaa atttcaccgc accgcccggt gagcgccagt taccgttgag
    21481 cgtttcgatc gctttcaccg cgacgcgcgt ctgttccggc gtcgcatccg gattggttaa
    21541 ccctttcttc aggtattctt cccagcgttg ctgcgaggcg gtgagataaa acgccggacg
    21601 cgccagaata tcgcggatct gcatttgctc tttgctaact tcctgagcgg tcagcagatg
    21661 ggaataggtg gtgtagagcg tggtcgaacc gttgatatgc gccttactgg taaagcgatt
    21721 accgttgatt tcagtctgca ccggcaggga tttatgcacc tgatattccg attcgccgga
    21781 ggtcagcaga tcccaggtgg cgcgcacttt gccaaaggtg actttcaggc catcacgggt
    21841 ggcgctgatt ttgcgctgat agtcagggta ttcgccagca atggttttat cggaaagcgg
    21901 tttcccttct ttcgcttcca gtttttccag cagttcgccg tcccacacca gatccagcgg
    21961 tttattgctg gtgattttgg tttccagtag tgacgtgcgc ggcgtggcga agcgcagagt
    22021 catttcgact tgcacatctt ttgctgtcag tttttgcacc agcgcaccgg gaatactgta
    22081 tgcctccagc gtaaagtcga ctttcttgcc gtcctgccag acggtcaggc ggtcgaaatt
    22141 gctggccata aagttgatgt actcttccgt cagcagcgca acgcccggaa agccgcccat
    22201 ggtgttaggg ccgtctggca acagatgacc atgccaggct ccgagatcga aaaacggatt
    22261 aaaacgctgg tgatcgtcgt aatcgtaatc cttcatgtac tgcggcgcgc cagtacggtt
    22321 aatcacgttt ttataattgt cggcgttagc ggcatgggcg gaaaaactta tcagcagagc
    22381 gcaggttact ggcgttaaaa tagtttttat tttcatgaaa ttacagtcct tattaccagt
    22441 agaaatattg catcaggaag atttggtcgc tgggatcgtg agaggcattt ttcatgtaga
    22501 agcggctatc aaaagccgtg gcgaaacgtc cgccgccgta ttcgtaccag ataccaaact
    22561 gcatgaaaga ggtggtttca tcgtcagcgt ggtcagggcg atgtttggcg taggtataag
    22621 cggtggagag ataaacgccc tttaacgctt catacatggc gctcaccatc atcccacttt
    22681 cacttcccgg tacgtccagc ccgtcggcgc gtccggtatg gtgccaggcg cgagcggcaa
    22741 agttaggagt tagcaggccc gtgagataga gctgtcctgt gccgtcggtg atttccagac
    22801 cgttcatcca ggtgatgcct tcggcaaggt catactggat gtaaccgttc atcattgccg
    22861 gataggtgta tttgtcgtca tagcgatcgt acttgccaaa atgtagccag gctttgcttt
    22921 cgtcatgatg tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct
    22981 gcaccttcag cgcggtcata atgtcgcggg tattgggaat gacgtagccc agatcggggg
    23041 tgaagtcgcc ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga
    23101 tttcggtgcc atcattggta cgataaccgc catagaaacg gttaattccg ccctcgaaac
    23161 caccccagct atggtctggc acccaggcat tgccgtcgtg gtcggcctgc acggtccagc
    23221 cttcgccgta aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat
    23281 aggtaccgtc gctgtattta tgtttattct caccctctaa atccatatat ccaccaaccc
    23341 ctaattcgcc gtaaaaacga aatgaaggag aagatgtcga ggtaattgca ggtgtttttg
    23401 tgtcgtcatg ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg
    23461 gcagagcaag taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc
    23521 taaagaaaaa atacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt
    23581 ttccctctcc tcatccagga agagggaaaa tgcctgctca ttttttatta ttctgcttaa
    23641 tcaacattgc tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata
    23701 tatttactgt atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa
    23761 aaaatgatgg tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca
    23821 aatatcgcaa tcagcattga aacaacgata ataccggtgc ggcgggagcc catgcggaaa
    23881 tcgcgtggca ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac
    23941 ggcggaagca tggacgctgc ggcagtcata ttaataatag tattcatcag atcctgcacg
    24001 gtattggaac cgagcatcgg gataatcatc agcgggatga cgatcaggaa ctggatccac
    24061 gctgcgcgcg ccggaacgcc gttttcgttc agttcgacgg ttttcttacc aaagatgcct
    24121 tccgggattt cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg
    24181 aacatcgcgg taaaggacac cagaccgaca aagcgattca tcaacgcttc cggtagacca
    24241 aaatacgccg ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg
    24301 ctgctgacga agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc
    24361 ccggcgagga tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac
    24421 acagcgacgg actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac
    24481 gcccagttga agttcgggat catcgagtca acggtgatgg ggtcagcagg ctgtacgccg
    24541 ccaaccagcg ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt
    24601 gaagtgacgg aggtaattgg ccccaacatt ttcgccccgt tggtggaaac ccaggtggag
    24661 aaggcgaaca gcaccatact gataatggtg gtggcaaccg gcgtcataat atattcgtag
    24721 ccgaggaagg catacgaagc ataagcaata acgcgcggca acagtgaggt gaaaaagaac
    24781 aggtttacga accagtaggt ataggcagta ataaatgccc aacgtccgcc cagcgaactt
    24841 tttacccacg cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg
    24901 atcagacaga agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc
    24961 agtccaagct caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc
    25021 agcgagagca agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc
    25081 cgaaaatgtg tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt
    25141 tatggaaata accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc
    25201 agataaaccg atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcaa
    25261 cggtttctcc gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta
    25321 cctgtaatgt ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca
    25381 cttcaaaata gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtct gtctccacgc
    25441 ggtaagtcaa tgagtcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt
    25501 caatcgcttc aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt
    25561 ctaagttatc gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc
    25621 tgcgccgaac tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag
    25681 caacgtaaag ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct
    25741 gccccaggag ttggagccga ggccaagcag ctggtggtcg aggttcaggg tgatgtcatc
    25801 actgcgctgc agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagcgcca
    25861 ggcgctgaag ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt
    25921 cagtgccgtc cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc
    25981 gaacatggca tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc
    26041 gtagttttcg cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc
    26101 catggtgaaa ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag
    26161 cgccacgtta acctggccat cggcagcgat gcgccagatg taggtgcagc gcatcccgaa
    26221 gtcaaacacc ggcggggcaa taactgtgcg gctgatgatc aacacttcac catcgctctg
    26281 ttctacggca aagtcgcgca gatgttcctg catgatctgc aaatgattcg gttgccacag
    26341 cccttcgtac tcctgcttgt ggttgtcgat cattggcttg aagaagttga tctttggctc
    26401 gcgggtcagc agcgactcgc cattcacctg ccaggatgtc ggtttgccac tcatttttga
    26461 gaaggtgatc gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt
    26521 cagcggacgc gcattatttg gtgcgaaagg cactggttgc gcggtgtttt ccttcagcgg
    26581 gaactgataa gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt
    26641 caccgtaatg ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg
    26701 ggcttcgctg ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc
    26761 ttcgacgcgc acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt
    26821 ttcgactttc agctcgccgt gagtcagatc cagcgcgtgg atttttaccg gcgcgataac
    26881 ctgtttgtac tctttcaggc ccggtcccgg cgtctgatcg gaatagatca aaccatcaag
    26941 acagaagtta tagttgttgg gatagtcgcc gtagtcgccg ccgaatttat accagacgtt
    27001 gccgttgtca tcctgcgcct ggatcccgtg gtcgcaccat tcccagacat aatgtccctg
    27061 aatgcaatcg tgcttataga agacgttctg gtactccgtc agcccgcctg gtccgttccc
    27121 catcgcatga gcatattcac agatgatgcg cggcttcgga tgcgggtatt caccaaactc
    27181 attcatcagc ggcacgcggg tgtacatggt ggaaataata tcgaccactt cagcatcgcg
    27241 atcttcttcg taatgcacca gtcgcgtgtc atccagcgcc ttcgctgcgt ggtacatcgc
    27301 gcggatgtta cagccatagc cggattcatt gcccagcgac cagatgatga tcgacggatg
    27361 gtttttctgc gcgtgaatat ggcgaacaat gcgctcgacg tagacttttt cccactgcgg
    27421 atcgtcggta atacggctga tatcgccgac attagcaaag ccgtgcgatt cgacgtcggt
    27481 ttccgccatc acaaacaagc cgtagatatc acacagttcg taaaaacgcg gatcgttcgg
    27541 gtagtgagcg gtacgcacgg agttgatatt gtgctgcttc atcaactgga gatctttctc
    27601 gacgcgatcc attccaacgg cgcggccttt gcgatgatcg ttgtcgtgac ggttgacgcc
    27661 gtgcagcatc acataacggt tattgatcca gaacagaccg tcgcgcactt tgatatcacg
    27721 gaagccaacg cgttgtggca ccacttccag aacgttgccg ttggcgtctt tcagcgtcat
    27781 gaccagatgg taaagataag gggattctgc tgaccattgc tgcggctgtt cgacagtaaa
    27841 agcaaagctg gcgctggtca gtttttcaat tgccaaatga tcaatggcgc tgctgtgcac
    27901 cacgcgttcg ccatcaaaca gggtatattc cagcgtcgtg acgacagggg aggcggcgag
    27961 attttccagc accacttcgc aggaaagcgt ggcatcgcaa taggcttcgt caaagtcggt
    28021 acgcacggtg aaatcgttaa tatgcgttag gtgttttccg accagataaa catcgcggaa
    28081 gatccccgct gaccaccaca tatcctggtc ttccacgtag gtagagtccg cccactgcat
    28141 cacgcgcaca cacaacaggt tgtcgccggt tttaaccatc gcgctgatgt caaactctgc
    28201 ggtcaggcga ctgcccttgc tgaaacccac atactgaccg ttaacataga cttcaaaata
    28261 ggtttcgacg ccgtcaaatt taatcagcgt ctgtttaccc tgccagccgt cgctgagggt
    28321 gaaaatacgt tgataggcac cggttgggtt atcgctgggg acaaacggca catcgatggg
    28381 gaacggaaaa ccttcgtcgg tatattgcag tttgccgtga ccttccattt gccacatggc
    28441 ggggacggta atatgccccc agtcagccat taactcagag gtgaaggctt ctggtacttg
    28501 cagcggatgg tcaaaaaagt ggaaattcca ctgaccgctt aagggcagaa acaggttgct
    28561 ggtttcgcgg gcaaaggtac gcgcttgcgc aacagaatca tatgaaaaaa agtacgcacg
    28621 cggcgcaagt cggttttcgt gggtgagctg aatgttttcc cagcgattca tagggcctcc
    28681 cggtagagag aaatcggaac cattcatggt gatggtagtt tgtgtaattg ctggggagtt
    28741 tagtaaaatt tttactaaaa agtagcaggt aaatgcattt actttaactg gatcacgaaa
    28801 ataaacggca gcactgtccg gcaaaaacga ttgccggaca gtgtgtaagg ggatttaacg
    28861 ggtcgtgccg cgcagtttta atttgctggg aacgaagact aacagcggca gcgcgcgacc
    28921 atcgcgggct ttttcataca ccaggttaac gccctgactt cccatcatct cggaatggat
    28981 gcgcacggtg gagagcggcg gaaaggtaaa tcgcgcggtg gggatatcgt taacgctgat
    29041 aagcgaaata tcctgtggga tgttcaggcc tcgttcatga attgcccgca gtacgccgat
    29101 agcaatggaa tcggaagcaa caaacagtgc cttcggatag tcttcccgcg ccagcatttg
    29161 ttttgccagt tcataacccg acgaactgga aaaaccgccg cgccagatat cctcttcgcg
    29221 taccacttgt ttcagtcggc catattccgc aaaggcgacc tcacgaatat ccgccttgcc
    29281 aggctcatct tcaccgccaa taaaaccaat acgattaacg ccctggttga tatagaagtc
    29341 gatgatttct ttactgatgc gtgccagatc gatatccacc gcatcgtaac cgctgccggg
    29401 ttcgtgaaag tcgataaaac agatattgtc ggtcaacgca caggcggcgg cgcgcagggc
    29461 gggcgtgggt ttgccgacaa ttaaaatacc ggtgacgttt ttaatgtctg gtaatccgct
    29521 gtgttcataa cagttggtga gctcaatgcc cagcttttcg cactgagttt caatgccgtg
    29581 gcggatcgcc agatagtaag gatcgttgat ctccagctcc tgttggtagc tgtagatagc
    29641 cagaatatgg tgttggttga ctgcacctgt ctggagttta cgggcactac tggtcttgta
    29701 ctccagcttt tcggcgatct cgagaatgcg atgtttcgtc tcttctttca cattcaatgt
    29761 cggatcgtca tttaagaccc tggatactgt cgccagggat acgccagctt cgattgcgat
    29821 gtcttttagt gttgccatgt ttaccttttt tactttccct gatgtgcgcc cccattgtag
    29881 aggaagtgct tatcgggtca cctgttttca gtaaataaag cgcgtttctt tgacgctgta
    29941 acggtaacac agcggctcac gaatgtgatc acgcctgcat tctccatttt tagcctacag
    30001 gattgagaaa aacttttgct atagtttagt aaaattttac ccgaagggag gaaacaatgg
    30061 aaaccgtggc ttacgctgat tttgcacgtc tggaaatgcg cgtcggaaag attgtggaag
    30121 tgaaacgcca tgaaaacgcc gacaagctgt acatcgtaca ggttgatgtg ggggaaaaaa
    30181 cactgcaaac cgtgaccagc ctggtgccgt actacagcga agaagagctg atggggaaaa
    30241 cggtggtggt cttatgcaat ctgcaaaaag cgaaaatgcg tggtgaaacg tcggaatgca
    30301 tgctgttgtg tgcggaaacc gatgacggca gtgaaagcgt gttgttaacg ccggagcgga
    30361 tgatgcctgc gggcgtacgc gtcgtataaa aagatcggat ggcgacgtcg tatcgccatc
    30421 cgatttgata ttacgcttct tcgacactta ctcgcatggc cgccagcgcc ttacgcgccg
    30481 ctttgatcac cagttcacac tgttcaatgg tcagtgtcag tggcggttca atgcggatcg
    30541 ttttggcgtt attgagcgtt ccggccacca gtacgcgctg gcggaacatc tcgctggcaa
    30601 agttatagcc gatttcgtta tcaacaaact caattgccat caacatccct ttaccacgcg
    30661 cttcctgtac cagatcggga tattcccgcg ccagttgacg gaaaccgtcc agcaacatat
    30721 cgcctttttg ctcagcctga gccggtaagt tctgctccag caacacattg atggtcgcca
    30781 gcgccgccgc acaggccagc gggttgccgc caaaggtggt ggtatgcagg aatgggttgt
    30841 cgaacagaac tgaaaacacc tcttcagtgg cgatggtcgc gccaatcggc atcacgccgc
    30901 cgccgagcgc tttggcaagg cagaggatat ccggctgtac gttctcatgc tcgcaggcga
    30961 acatcttgcc cgtgcgcccc atgcccgttt gtacttcatc gaggatcatc agtgcgccga
    31021 actcatcgca tagcttacgt acggcggtga gatagcccgg cggcggcaga attacgccac
    31081 cttcaccctg aatcggttcg aggatcaccg cagccacatc atcaccggtt tttttgcact
    31141 cgttaagagc cgtgcgcatg gcttcgatat tgccaaacgg cacatgacgg aagcccggca
    31201 gcagtggcat aaacggtttg cggaaggtcg atttcgccgt ggccgacagc gcgccaagtg
    31261 atttaccgtg gaacgcgccg ctggtggcaa taaaagtaaa cttgccgcgc ggtgactggt
    31321 aagctttcgc cagcttcagc gctgcttcaa cggactcggt gccgctatta cagaagaagc
    31381 tgtatttcag tttaccgggc gttagcgcag caagggtttt cgccaacatc gcccgtaacg
    31441 gatcgagcag ctcctggctg tgcagcggtt gtttcgcaag ttgattctgt acggcggaaa
    31501 ccacaactgg attacggtgc cccacgttga aaattccaaa acctcccagg cagtcgataa
    31561 actcctgtcc ctgggtgtcg acaagcgtat ttaaacctcc cgcttgccac tctacggctc
    31621 cgtaatcccc gccggcggta acagatttgc gatactctaa aaaccccgga ttgacatgct
    31681 ctttgaagta ttcaatcacc tctcggttaa gtgctttcat ctcctcatga tccagcgttc
    31741 gcttctcaat gagattcagg gcgtgggcgc tgcacgctaa agccgatgcg ctcgaaggta
    31801 acctgttcaa aatatgctcc ggaggctcgc gtatcacatg ataccgattt aagtattgca
    31861 gggattgcgc cactccggct gcgatcgcga aaatcgggat aaacacaaat tcggttatca
    31921 tttgtgcaat atttaatgct atctattaac atttgttgtt attttgcacg gtttttgtcg
    31981 ggtatggatt tgatgattgc tgcgcaaatg cacgttttca gggcaataat tgcctgattt
    32041 ttgggcaaga taattaacgt tttcgtatag taacagagtg aaagtatttt aaatcaaaga
    32101 attagatttg tgctttaatc gcaaattgcg atctaaatca aattaatcgg ttaaagataa
    32161 ccgcagcggg gccgacataa actctgacaa gaagttaaca accatataac ctgcacagga
    32221 cgcgaacatg tcttctcatc cgtatgtcac ccagcaaaat accccgctgg cggacgatac
    32281 cactctgatg tccactaccg atctgcaaag ctatatcact catgctaatg acacttttgt
    32341 gcaggtgagc ggctttacct tgcaagagtt acaagggcag ccgcacaaca tggtgcgtca
    32401 cccggatatg ccaaaagcgg cgtttgcgga tatgtggttc accctgaaaa aaggggagcc
    32461 ctggagcggc atcgtgaaaa atcgccgcaa aaatggcgac cattattggg tgcgggccaa
    32521 tgcggtaccg atggtgcgcg agggaaaaat cagtggctat atgtcgattc gtacccgggc
    32581 gacggatgaa gagattgcgg cggtggagcc gctgtacaaa gcgctgaacg ccggacgtac
    32641 cagtaagcgt attcataaag gcctggtggt gcgtaaaggc tggctgggta aactgccttc
    32701 attaccgctt cgctggcggg cgcgtggagt gatgaccctg atgtttatct tgctggcggc
    32761 catgctttgg tttgttgctg ccccggtggt gacgtatttc ctctgtgtgt tagtggtatt
    32821 gttggcaagc gcttgttttg aatggcagat tgtgcgcccg atagaaaatg tcgcccgtca
    32881 ggcactgaag gtggcgaccg gagagcgtaa tagtgttgag cacctgaatc gcagcgatga
    32941 gctggggctg acattacgcg cggtagggca gcttggcctg atgtgccgtt ggctaattaa
    33001 cgatgtctca agccaggtgt ccagtgtcag aaatggcagt gagacgctgg cgaaaggcac
    33061 cgatgaactg aacgaacata cccagcagac agttgataac gttcagcaaa cggtggcgac
    33121 catgaaccaa atggcggcgt cggtgaaaca gaactctgcc acggcgtcgg ctgccgataa
    33181 actttctatc accgccagta atgcggcagt gcagggtggg gaggcgatga ccacggtgat
    33241 caagacaatg gacgatatcg ccgacagtac ccagcgcatt ggcaccatta cttcgctgat
    33301 taacgatatt gcgtttcaga ccaatattct ggccctgaat gcggcggtgg aagcggcgcg
    33361 tgccggcgaa cagggcaaag gttttgcagt ggtggcaggg gaagtgcgtc atttagccag
    33421 ccgcagcgct aatgctgcca acgatattcg caagctgatt gatgccagtg ctgataaggt
    33481 gcaatccggt tcgcagcagg tacacgccgc cggacggacg atggaagata ttgtggcaca
    33541 ggtgaaaaac gtcacccagt tgatcgccca gattagccat tcaacgctgg aacaggccga
    33601 tgggctttcc agcctgaccc gtgcagtgga tgagcttaac ctgatcaccc agaaaaatgc
    33661 cgagctggtg gaagagagtg cgcaggtgtc ggcgatggtg aaacaccgcg ccagccgact
    33721 ggaagacgcg gtgacggtgc tgcattaatc ggtatgccgg atccggcggt tggagcacaa
    33781 tgcctgatgc gatgctggcg catcttatca ggcctgcggg gtgtgtagcg ggtgtaggcc
    33841 ggataaggcg cgtcaagcgt cgcatccggc aattgcaccg cgccactggc ggatgcggcg
    33901 attaatccag ttgcgtaata tcaaaagcgg cgcggtcgat caccgcaatg atctttttta
    33961 tttgtgcatc actgatatcg ctctggttga cgcgtaaatc cagcaccgct ttaaaattat
    34021 ccagcgcccg cttcatttgc gggttctggc ggagtgcagc accaacgcaa cgcgctttga
    34081 tgcgttcttc aatcatctcc acctgttcgc ggttttcttc cagccactgc gcgccttgtt
    34141 cggtcagcgc aatctgcttc ttaccacctt cctcttcgcg gatggtaatc agcgactgct
    34201 cctgcagaaa atccagcgtt gggtagatga cgcccgggct tggggtgtaa ttcccctggg
    34261 ttaggttctc aatcgcttta atcaattcgt aaccgtggct gtcatcgcgc gagagaatat
    34321 ccagaatcac cagacgcaat tcaccgtgac caaagaaacg ttgccgacga ccgccgccgc
    34381 gaccgtggcg accaccgcag cattgaccgt gttcatgctg gtgaccgtgt ccgcagtgct
    34441 cgtgttctga cttctcacct ttgcagcagc cctcatggcg tggctggcct tcatgtttac
    34501 aacacccttc gtgatgatgg ctcatatcag cctccttttg cttgattaga tatatctaat
    34561 ttatatctga tgtgagcatt actgcaagta caaaaatgat ttttaaacgt ggaagcacct
    34621 cacatccagg ccggataaga cgttcacgcc gcatccggca gcattgctat ggcactgaat
    34681 gccttcacat acttcagata tatcagttct acaaatcgct tgcatttatc atgattaaca
    34741 atcattatca tttacgaaaa ttatttagat atatctgatt actatcacga aggcgataac
    34801 aatgaataac tccccccgct acccgcagcg cgttcgcaat gatctgcgct tccgtgaact
    34861 gactgtgtta cgcgttgagc gcatcagcgc cggttttcag cgcattgtcc tcggcggcga
    34921 ggcgctggac ggttttacat cgcgcggctt tgacgatcac agcaaactct tctttcctca
    34981 acctgacgct cactttgtgc cgccaacggt aacggaagag ggcatcgtct ggccggaagg
    35041 accacgccca ccgtcgcgtg actatacgcc gctgtatgac gaactacgcc atgaactggc
    35101 gatcgatttc tttattcacg acggtggggt cgccagcggc tgggcgatgc aggcgcaacc
    35161 gggcgataaa cttacggtgg caggtccgcg cggttcgctg gtggtgccgg aagattacgc
    35221 gtatcagctg tatgtctgcg atgaatccgg aatgcccgca ctgcgccgcc gcctggaaac
    35281 gttgagcaaa cttgccgtta aaccgcaagt tagtgcgctg gttagcgtgc gggataacgc
    35341 ctgtcaggat tatctcgcgc accttgatgg ttttaatatc gaatggctgg cacatgatga
    35401 gcaggcggta gatgcgcgtc tggcgcagat gcaaatccct gccgatgatt acttcatctg
    35461 gataaccggc gaaggtaaag tcgttaagaa tttaagccgc cgctttgaag cggaacagta
    35521 tgacccacag cgggtacgtg cagcggctta ctggcacgca aagtaactga tatataaaaa
    35581 gaaaaaggct gacgatttct cgtcagcctt tgctatatct ggtggcccct gctggacttg
    35641 aaccagcgac caagcgatta tgagtcgcct gctctaacca ctgagctaag gggccatggt
    35701 agcggattat aaagtaactc cgtgtcgcaa tccagccatt accgtgcgcc tgctgttttt
    35761 ataaacaatg tattttcaat ccgttatact tttcctggtg atgttagaaa ggagtaaata
    35821 tggttgagga tattttggct ccagggttac gggtcgtgtt ttgcggtatc aaccctgggc
    35881 tttcatccgc cgggactggt tttccctttg ctcatccagc aaatcgcttc tggaaggtga
    35941 tatatcaggc cgggtttacc gaccgtcagt tgaagccgca ggaggcacag catctgctgg
    36001 attatcgttg tggcgtcacc aaactggtag accgtccaac ggtgcaagcc aatgaagttt
    36061 caaagcagga gctgcacgca ggtgggcgta agctgattga aaaaattgaa gattatcagc
    36121 cgcaggcgtt ggcgattctg ggcaaacaag catatgaaca gggattcagc cagcgcggtg
    36181 cacagtgggg gaaacaaacg ctcaccattg gttcgacgca gatttgggtg ctgccaaatc
    36241 ccagcggttt aagtcgcgtt tcactagaga aactggttga agcgtatcgc gagctggacc
    36301 aggcgctggt agtgcgtggg cgataaaaaa cgccaccgaa aaggtggcgt ttgtgcaaag
    36361 ggactagtgc cgggtgcggc gtaacgcctg atccggccta ccgattaatc gtccaggaag
    36421 ctacgcaaca cttcagaacg gctcgggtga cgcagtttgc gcagcgcctt cgcttcgatc
    36481 tgacggatac gttcgcgggt aacgtcgaac tgtttaccca cttcttccag cgtgtggtcg
    36541 gtgttcatat cgataccgaa acgcatacgc agaactttcg cttcacgcgc ggtcaggcca
    36601 gccagcacgt cgtgcgttgc cgcacgcagg ctttcggtgg tcgcagaatc cagcggcagc
    36661 tcgagggtgg tatcctcgat gaaatccccc agatgcgaat cttcatcatc accgatcggc
    36721 gtttccatgg agattggctc tttggcgatc ttcagcactt tgcggatctt gtcttccggc
    36781 atcagcatac gttcagccag ttcttccggc gtcggttcac ggcccatctc ttgcagcatc
    36841 tggcgagaaa tacggttgag tttgttgata gtctcaatca tatgcaccgg aatacggatg
    36901 gtgcgcgcct gatccgcgat agaacgggtg atcgcctgac ggatccacca ggttgcgtag
    36961 gtggagaact tgtaaccacg gcggtattcg aatttatcaa ccgctttcat cagaccgatg
    37021 ttgccttcct gaatcaggtc aaggaactgc aagccacggt tggtgtattt cttggcgata
    37081 gaaataacca gacgtaagtt cgcttcaacc atctctttct tcgcacggcg ggctttcgct
    37141 tcaccgatgg acatacgacg gttgatatct ttaacctgct cgatggtcag gccggtttct
    37201 tcttcaatct gctgcagttt ctgcagggcg cgatgcactt cttcagagac atcgtgcagt
    37261 ttttccgacc acggcttgtt catcgcaatt gccgcgttga accaggtatc gctggtttcg
    37321 ttgccggtaa acagggtaat gaagtttttc ttcggcattt tgcactgctc aacgcagagc
    37381 ttcatgatca gacgttcttg cgtacgaacg cggtccatca tgacgcgcat gctgttgacc
    37441 aggtagtcaa actgcttcgg caccaggcgg aactgtttga atacttcaga cagtttcagg
    37501 atctcttcct gagcggcagc gtgactgcga cctttcgctt tgatggtgtc acgcgttaca
    37561 acgtactgag cgcgtagttc cgcaaatttt tcgcgagcca gttccgggtc gatgctgttg
    37621 tcatcatcgg cgctgtcgtc atcgccatct tcttcgtctt catcttcgtc atcgtccaga
    37681 tcttcctggg aaagctcaga accgacgtga gtggcggtag gtgccagatc ttcttctgcg
    37741 ttcgggtcaa caaagccggt gatcagatcg gacagacgcg cttcttctgc ttcaacacga
    37801 tcgtactgtt ccagcagata ggtgatcgct tccggatatt cagcaacgga gcattgaacc
    37861 tggttgatcc cgtcttcaat acgcttagcg atgtcaattt cgccttcgcg ggtcaacagt
    37921 tcaacggtgc ccatttcacg catgtacatg cgtaccgggt cagtcgtgcg cccgatttca
    37981 gattccacgc tggaaagcac ctgcgcggcg gcttcggcag catcttcgtc cgcggtgttt
    38041 tcagccagca tcagatcatc ggcatccggt gcttcttcca tcacctgaat gcccatgtcg
    38101 ttgatcattt ggatgatgtc ttcgatctga tctgaatcga cgatatcttc cggcagatgg
    38161 tcattgacct cggcataggt cagatagcct tgctccttac cacgggtgac aagaagtttc
    38221 agctgtgact gcgggttttg ctccataaga cggtatccac acttatttca tgaggttggt
    38281 gttgggcgat tgacccgatg tccttcagcc gttaacgctg aaatccaacg gttgtttacc
    38341 gccaacgata attacgaggg cgtacttata tatttgccgc tgcctctcag tgcggctgtc
    38401 gggggcttcc cgatcgctct tcggcactta agccgttaaa tcactttttc gccagctcct
    38461 ggtttaatgt ccagagctcc aggcgttctt cgttgcttaa accatgcgtg cgctcacgag
    38521 cgattaactc ttcctggcgc agttcaagca gcgaatcaaa catatggttg agtgagtcgg
    38581 tgaaggtttg ctcagcaata ttcttatctg ctatatcgtc ccacatcgac agtttttcaa
    38641 gggtggcagc attatttgta ccacgatagt gctctaaaag ttgcccggtg gtcagacctg
    38701 gctgggagag acaagtgttg accagttctc tgaataagcc aagtccaggg agcttatttt
    38761 catccagatt ctcaagcggc gggaccaacg tcgctaattc tggattttgc accagcaacc
    38821 ctataagtat acgcatggtc gtgcgtttta gctgcggaac agggcgagaa acgccgctct
    38881 ctgccgcttt tggcattaat cgttcaagct ggctgtcatc aagtatgcct aatttgttgc
    38941 ctaattcctg acgaagatat attcgcagcg tttcgcccgg cacttgcgat atcaatggca
    39001 gtgccagcgt actcaaacgt gcgcgcccgt caggggtact cagatcaact tgcggcatca
    39061 ggctgttaaa cagaaatgcg gaaagcggca tcgcctgctc catccgcgct tcaaacgctt
    39121 ctttaccttc ttttcgtacc agcgtgtcag ggtcttcgcc atccggcaaa aacataaagc
    39181 gtagctgacg gccgtctgtc atgtaaggca gcgccgtttc cagcgctcgc catgcggcat
    39241 cgcggcctgc acggtcgccg tcataacagc aaatgacatt gttggtcgcg cggaacaaca
    39301 gttgtatgtg atcggcggtg gttgacgtac ctaacgacgc aacggcgtaa ttaatgccgt
    39361 attgcgccag cgccaccacg tccatatagc cttcgaccac aagcagacga ttgggttcag
    39421 cgttatcctg ctgcgcttca taaagaccgt aaagctggcg gcctttatgg aaaatgtctg
    39481 tttccggcga gttcaggtat ttgggggtat cgttgcccag cacgcgcccg ccaaaaccaa
    39541 tcacccgacc gcgtttatcg cgaatgggga acatcacccg ctcgcggaaa cgatcgtaac
    39601 tgcgtccctg atcgttagtg accaacatcc ccgcatcaat caatgactgg cgattttctg
    39661 gattgccgcc aaaccgcttc aggacgttgt cccagccggg gggcgcaaaa ccaatcgcaa
    39721 agcgagcgat aacctcgtgg cttaatccgc gtttttccag atattggcgc gcagacgtgg
    39781 caacaggttg ttgtaaagat tgttggtaaa acgtattcag accgtccatc aactgataaa
    39841 gcgtttgcct ctgatggcgc tcgatctggc tggggccgct gcctgcttca aatggcactt
    39901 caagattgtg cattgctgcc agctcttcga ccgtttcaac gaactcgagc ttgtcgtagt
    39961 tcatcaggaa gtcgatcgcg ttgccgtgcg cgccacatcc aaagcagtgg taaaactgtt
    40021 tctcaccgtt aacggtgaat gacggggttt tctcgttgtg gaatggacaa cacgcgtgga
    40081 aattcttgcc ctgctttttc agcttcacac gggcatcgat cagatcgacg atgtcagtgc
    40141 gtgccagcag atcattaatg aatacgcgtg ggattcgtcc agccataggc cccgattttt
    40201 agcaattcat aaacgaaaat aagccgcgca ttcctttcgg aagcacggcc ttacaactac
    40261 aactcggtct gatcggagag caacgctctc ggggaattag tacagacgag tgcggcgtgc
    40321 gttttcgcga gccagtttct tcgcgtgacg tttcactgca gaagctttag cgcgcttacg
    40381 ttcggtagtc ggtttttcat agaactcacg acgacgaact tccgccagaa cacctgcttt
    40441 ttcgcaggaa cgcttgaagc gacgcagagc tacgtcgaac ggctcgtttt cacgtacttt
    40501 aattaccggc atgtgcctct cacctttgat taattcggtt tgccgctggc atcaacgcca
    40561 gcttatttca aaatggtgcg gaattttact gcaattgctg ctgctttgta aagcaccgcg
    40621 gcctttttga aagggacttt tataagggtg aggagtatac acgaggcttt ctccaggggc
    40681 gaacaaagtt ttacatcaac ccgcattggt cctacactgc gcggtaataa agcgaggtaa
    40741 aacaagtcat gcgtgtactg ggtattgaaa cttcctgcga tgaaaccggc atcgccattt
    40801 acgacgatga aaaaggtttg ttagccaacc aattgtatag tcaggtgaaa ttgcacgctg
    40861 actacggcgg cgtcgtgcct gaactggcct cccgcgatca cgtgcgcaaa accgtaccgt
    40921 tgatccaggc ggcgctaaag gagtctggct taacggcaaa agacattgat gctgtggcct
    40981 ataccgcagg ccctggatta gtcggcgcac tgctggttgg cgcaaccgtg gggcgttctc
    41041 tggcgtttgc ctggaacgtt ccggcaatcc cggtacacca tatggaaggg catctgttag
    41101 cgccgatgct ggaagataac ccgccggaat ttccgtttgt cgcgctgctg gtgtccggcg
    41161 gtcatacgca gttaatcagc gtgactggca ttggtcagta cgagctgctc ggcgagtcta
    41221 tcgatgatgc cgccggtgaa gcgtttgata aaaccgcgaa gctgctgggg ctggattatc
    41281 ctggcggacc gttactgtcg aaaatggcgg ctcagggtac tgccgggcgc tttgttttcc
    41341 cgcgtccgat gaccgaccgt ccggggctgg atttcagctt ttctggtctg aaaacctttg
    41401 cggcgaacac gattcgtgac aacggcaccg acgaccagac gcgtgctgat atcgcccgcg
    41461 cctttgaaga tgcggtggtc gatacgttga tgattaagtg taagcgagcg ttggatcaga
    41521 cgggctttaa gcgactggtc atggcgggcg gcgtgagtgc taaccgcacg ttacgggcga
    41581 agctggcgga aatgatgaaa aaacgccgcg gcgaagtgtt ctacgcgcgt ccggaatttt
    41641 gtactgataa cggcgcgatg atcgcctatg ccggaatggt gcggtttaaa gcaggcgcga
    41701 cggcggatct cggcgttagc gtgcgtccgc gctggccact ggcagagtta ccggctgcgt
    41761 aaaactatcc ggtgccggag agcaatttcc ggcaccgtcc tcacttaaag caacaccacg
    41821 ggcatccaca gcaagccggt aatcaccagc aacacgagga atatcagtcc aaaaatcgcc
    41881 cccagtcgcc agtaatccac cgttggcaga taaccactac cgtagtaaat cggactgggt
    41941 ccggtggcgt atggtgtaag aatgctcccc agaccaattg ccgcgccaac catcaggcag
    42001 aacaccggca gcgggatttc cggcatcgcc agcgcggcgg caatcatcat cggtgcgagg
    42061 gcggaggtat acgccgtggc gctggcgaaa aagtagcgca gcagataaaa caccacaatc
    42121 aacgccacca tcaccatcgt tggcgaataa ccgcttaagc tgcctgctaa cagtttgcca
    42181 aaccagctaa taaaaccagt gttgttgagt ccggtagcga gggtgataag cgaggccagc
    42241 cagaagaaaa cgttccacgc cgctttatta ctgacaatgt cgtcccaact gataatgcgc
    42301 agaagcagca tcagtgccac cacgctgtaa ccgaccatcg cggcatcgat ataatcaccg
    42361 ccgaaaatcc acagcaccag cgcgcctacc atcagcccca gcatccgttt ttcacgcgaa
    42421 cagagcgggc ccattgcctg cagttccgtc tctgcccagc gcggcacctg atcgccagac
    42481 ttcagtaccg gcgggtacag cacataagcc agccagggaa ccagcagcac cagcaaaata
    42541 ctgagcggca acatcccgag gaaccagtcg ccccaactca gcgtggcgtg agatgcgctt
    42601 ttcatcagtc caattaacag caagtttggt gccatcgccg tcaagaaaat ggcgctggtc
    42661 acgcagtcgg caacaatccc catccacatg atgtacgagc caatgctgcg cgaactgctg
    42721 tcgtttggtt gtgattgata gagcggtggc aggttacgga tgatgggata gataatcccc
    42781 gcaccacgcg ccgagttgga cggtgttaca ggtgcgagga tcagctcgga gaacatcacc
    42841 gcatagccga gaaacagcgt gcgatgtccc atctttttca ccagaatcag cgcaatgcgg
    42901 cgtcccagcc cggttttttc atagcctgtg ccaaacataa aggcggcgaa aatcagccag
    42961 ataaccgaat tagaaaaacc ggaaaccgcc cacgagaggg attttgcagt gaatttaaag
    43021 cctggctggg cgagctgttc ggggctgaac agcagccagg gagagagtat ggcgatgatg
    43081 gagataccca ccatcgccac cacggcaccc ggcacgggtt cgaggatcag tccaacgatc
    43141 acgccagtaa aaacggcaaa gtagagccag gtatgactct ccagccctgc gggaaccgga
    43201 attagagcaa taatggcgat gaccgccagc ggcgcaaggt atcgccacca ttcagtggaa
    43261 ggtttcatcg tgttatgcct gcgaaaaagg agagggggcg agcctctccg ggagggttat
    43321 ttgatgtaat ggacgtgctc gcagatctct tccacgatgg gatcgcggcg ttcggcgaac
    43381 agctttttgt tttcggctat caggttgttg ccgtgggtat caatagagac aatcagcggg
    43441 ccgaactctt tgacccggca gacccacagt gactccggca tcccgagttc tgtccagtgc
    43501 acttcttcaa tctcttccac ctgggttgcc gccagcaccg cgcagcctgc cgggaaaatc
    43561 acatgtagcg ccttgaattt ctggcagcct tcttcggtca gcggccccat tccgcctttg
    43621 ccaaccacca gtttcacgcc ggtctgctcg ataaattcac gttcaaaact ttccatacgc
    43681 atactggttg tcgggccaac ggaaaccatc tcccatttat cgccgttttt gcgcacgatg
    43741 gggccagcgt ggaaaatcgc tttgccgttg agatcgtaag ggatcggacg tttcagttcg
    43801 atcaagcggc ggtgacaaac gtcacggcag gtcaccagcg taccggtcag gtagatcaca
    43861 tcgccgacgc gaatatcttg cagatcttca gctttgatcg gggttgtcag gatctttttc
    43921 ataacgcgct ccgggtgtga gacagatttt caaaggtgag atcggcatga accagcagcg
    43981 tgccgcgacg atgcgcccag cagccggtag agacagcaac gccgatggtt gacggatggc
    44041 gggcggcaga ttcgatatgc acgcccatca ctgaactgtt gccggtcagc ccttgtggac
    44101 caatccccag acggttgagt ccttcttcca ggcgtagctc cagctctgcc gcttttggat
    44161 tgggatggcg gctgccaatc ggacgcaaaa tggctttacg cgagagtacg gcggcggttt
    44221 ccaccgaggt ggcgatgccc acgcccacca gtaccggtgg acaggcgttt acggcgaggg
    44281 tggagatatt ttcgaagacg aatttcacca cgccttcgta gccttctgac ggcattaaca
    44341 ctttcgagcg gccaggtagc gtgcagccgc cgcctgccat gtaaacttcg atttccgcat
    44401 cgtcattgtc ggggatgatg tcccaggtga cccacggtac gccgctaccg gtatttttgc
    44461 cggtgtttac ttcgtcaaaa atttctaccg cattgtgacg tagtggcgct ttgacggttg
    44521 cctcttccac ggcttgtttg agtatgcttt gcagctcgcc aagcagtggg aagcgggaac
    44581 cgactttaac gaagaacata atctccccgg tgtcctgaca ggcaggacga ttcaggtcaa
    44641 tcgctttttg catgttgtcg aacatcgtat ggtagataat tttccccatc gacgacgttt
    44701 cggcatcctt tagctgtttt agtttatcca ccacgtcatc aggcattcgg gtagaaatca
    44761 tggcggtaaa gttagcgaca atctctgtca acttattcac tgcctgttgc ttattacttt
    44821 cgctcatcat ggcaactcca gtttttgtat ttgtctggaa aaattcaccg ccacaaacta
    44881 ccactaaaaa tgtggtttta tattttttgc caccgtgaat atgtaattaa cagttcgtga
    44941 atcaacgcga aaataagaat gaatattgcg ccagagcagg gaatggcaat gttttgcgaa
    45001 atacactgcg cttatcacaa ctgcggataa ttaacgatgc tgaatagctg gcctttagcc
    45061 aaagacttgc aggtactggt agaaatcgtc cacagcggga gttttagtgc tgcagcggcg
    45121 acgcttgggc agacgcctgc atttgtcacc aagcggatcc aaatccttga aaatacgctg
    45181 gcaacaacgc tgctgaaccg ctccgctcgc ggcgtggcgc tgactgaaag tggccaacgt
    45241 tgttacgaac atgcgctgga aatcctcacc caatatcagc gactggtcga tgacgtcacg
    45301 caaatcaaaa cgcgcccgga ggggatgatt cgtattggtt gcagttttgg ttttgggcgc
    45361 agccatattg cgccagccat taccgaactt atgcgcaatt atcctgagtt acaggtgcat
    45421 tttgaattgt tcgatcggca aattgattta gtgcaggata atattgatct ggatattcgt
    45481 attaatgacg aaattcctga ttattatatt gcgcatctgt taacgaaaaa taaaagaata
    45541 ttatgcgcag cacctgagta tctgcaaaaa tatccgcagc cacaatcatt acaggaatta
    45601 agtcgtcatg actgtctggt gaccaaagag cgcgatatga cccacggaat atgggagttg
    45661 gggaacggtc aggagaaaaa atcggtgaag gttagcgggc atctttcctc caatagcggc
    45721 gagatagtgt tgcaatgggc gctggagggc aaagggatta tgctgcgttc cgagtgggat
    45781 gtgttgccgt ttctggagag tggcaaactg gtgcaagtat tgccggagta tgcacaaagc
    45841 gccaatatct gggctgttta ccgggagccg ctctatcgca gcatgaaatt acgtgtctgc
    45901 gtggaatttc tggcggcatg gtgccagcaa cggctgggca agcccgatga aggctatcag
    45961 gtcatgtaga tccaccagaa atcactcggg atccttttcg cgctttcttt tgaatttcgt
    46021 ccagattttt gtctcctgac gacgccacag acgctggatg ttgtcatgat gacgcagcag
    46081 gatcaggcaa gagagcatcg aaaccgggaa ggtgaattgt ggcttaaacc accatacata
    46141 aaacggagca atcagtgcac tgacaatcgc tcccagcgac gagtatccgc tcaatagcac
    46201 ggtcagtaac caggttcccg ccattactcc ggtgagatcc cagccaatgg gtgcgatggc
    46261 accaaaagcg gtagcaacgc cttttcctcc tttaaatccg aagaaaacgg gccagatgtg
    46321 tccaagacag gcggcgatgg caattaagcc tagccagaag gggctgacac ctaattcata
    46381 cgcgccccag acgggcaaca ttcctttcag aacgtcgaaa atcagtactg ctacggctgc
    46441 tcccttgcca ccgatacgta acacattggt tgcgcctgga ttgccggagc cgctggttcg
    46501 cggatcgggc agcccacaca agcggcaaac cagaatggca ctggaaatgg agccgcagag
    46561 gtacgcgatg aggatcattc caggcgcgat tgcactcata agctgttccg ttttgaaaat
    46621 tcgtgttcaa cgatgaatct gtggataata cgcacatttc gccggaagtg gtatccggtt
    46681 agccaaaaag caggcaggac gtgatggata ttgtatttat agagcaactt tcggtaatca
    46741 ccactattgg tgtttacgac tgggaacaga ccatcgaaca gaagttagtg ttcgatatcg
    46801 aaatggcatg ggataaccgt aaagcggcga aaagtgatga tgtggcggat tgcctcagtt
    46861 acgctgacat tgcagaaacg gtggtcagcc acgtcgaggg ggcgcgtttt gcgctggtgg
    46921 aacgcgtggc tgaagaggtg gcggagctgc tgttagcacg cttcaactcg ccgtgggtgc
    46981 gtatcaaact cagcaagcca ggcgcagtgg cgcgggcggc gaatgttggc gtaatcattg
    47041 agcgtggcaa taatctgaaa gaaaataatt aattttacag ctgttaaacc aaacggttat
    47101 aacctggtca tacgcagtag ttcggacaag cggtacattt taataattta ggggtttatt
    47161 gatgagcgat atgcactcgc tgctaatagc ggcaatattg ggtgtggtcg aaggattgac
    47221 agaatttctg ccggtatcca gcacgggcca tatgattatt gtcggtcact tgttggggtt
    47281 tgagggcgac acggcgaaaa cctttgaagt tgtgatccag ttaggatcaa ttctggcggt
    47341 agtagtgatg ttctggcggc gtctgtttgg cctgattggc atccactttg gccgcccgtt
    47401 gcagcacgaa ggtgaaagca aaggtcgttt aacgctgatc cacattttgc tggggatgat
    47461 tccggcggtg gtactggggc tattgttcca cgacacgatt aagtcattgt ttaacccgat
    47521 aaatgtgatg tatgcgctgg tcgttggcgg tttgttgctg attgccgccg aatgcctgaa
    47581 gccgaaagag ccgcgtgcgc cgggtcttga tgatatgacc tatcgtcagg cgtttatgat
    47641 tggctgtttc cagtgtctgg cgctgtggcc gggtttctcc cgttccgggg ccaccatttc
    47701 aggtgggatg ctgatgggcg tgagccgtta cgctgcttcc gagttctcgt tcctgctggc
    47761 ggtaccgatg atgatgggcg caacggcgct cgatctctac aaaagctggg gcttcctgac
    47821 aaccggcgat atcccgatgt ttgccgttgg gtttatcacc gcttttgtgg tggcgctgat
    47881 agcgattaaa accttcctgc aattgattaa gcgcatttcg tttatcccgt tcgccattta
    47941 tcgctttatt gtggcggctg cggtgtacgt cgtgttcttt tgattgagaa aaggttcggg
    48001 agcaggcctg gttttctccc tcgctctgta gggagagggc cggggtgggg gtgattgttc
    48061 gcaccggtgc tggtcggttc ccctcaccct aaccctctcc ccgaaggggc gaggggatgg
    48121 ttcgtgttaa cgtttagttt tatactcagt caggctttgg gcaacgttgt tccttccaac
    48181 tggctaccgc cgcaatccgc cgtcgggtca gctcctcgcg aatctccaca cctttaaatc
    48241 ccgcttcaac gaccgctttt gtcggcactg actgcgccac ttcccaggct tcgcgcaacc
    48301 aacggccttg cgggtagtcc gctgattcaa aaccggttct gccgcgtacg tcagcctcgc
    48361 tggtcagcgc cagttgctcg acacgctgcg gtttacgcca ggcatcgatg gaatcaaata
    48421 atttgacgat ggtttttggg ttcagcattg ggaaggtgtg gatgagatcg tgaaactcag
    48481 ccaccagtct ggctaaatcg cgaatttcat ttggcacgcg cagacgctgg catagttgtt
    48541 ccactaactt aacacccgcc gggccatgcc catgatgacg cggccaaagt tctggcggcg
    48601 tcagtccttt accgagatcg tggcataaag ttgcgaaacg gacatcaacc tgcggactca
    48661 gcatcgccgc cattgagagc gtcattaagg tatgaatacc cgtgtcgatt tccggatgcc
    48721 acctggcagg ggccggaacg ccaaacaggg cgtcaatttc cgggaataaa acgcgaagtg
    48781 caccgcaatc gcgcagtacc tggaagaaca cctggggatt gcgggtggtg agggcgcttt
    48841 ccgtctcttt ccatacccgt tcaggcgtca ggtgttccag ttcacccgca tgggtcatct
    48901 cgcgcatcag cgtcagagtt tcatcggcaa tacgaaaacc gaggtgggca taacgcgcag
    48961 caaaacgcgc cacgcgcaga acgcgtaacg gatcttcgcc aaaagcgggg gaaacatggc
    49021 gcaacagacg attttgcaga tcgcccagac cgttgtacgg gtcgataatc tcgccgttat
    49081 cgtcctgagc cagcgcatta atggtcagat cgcgacgctt aagatcatct tccagcgtga
    49141 catccggtgc ggcatagcaa gtaaaaccgg tgtaaccgga accggatttc cgttcggtac
    49201 gtgccagcgc atactcttca tgcgtttgcg gatgcagaaa cacaggaaaa tcgcggccta
    49261 cctgctggta gcccgcgtcg agcatctcct gtggcgtact gccgaccacc acccaatctc
    49321 tgtctttgac cggtagccct aacaatgcat cccgaacagc accaccgacc agataaatct
    49381 tcacgccatc atcccttcgt caaatacaca attcctaata ataagacagt gcaacgaaga
    49441 aggcgattta gttcatccag cgatctttgc gtttgcgcgt tgggagcagg tgtggcagta
    49501 ccagaccgag cagcaagcca agccccagca cgccaccgcc atacataaac cattgcatga
    49561 tgatggtgcg ctgtttgtca tccagctgta cgctggcggc atcgaccttt ttctgcgcga
    49621 caatcagctc gtttttcaat ttctgatttt cttcttttaa tccgttgatc acgctgtcgc
    49681 tctgcgccac tttttgctgc atttctgcgg tgcgctgatt ccaggtgtta tcgatattgg
    49741 tgagtttatc ggtcagggtt ttgacctgat tttccagatc tggcacacgg gagcgcaggc
    49801 ttggctcagt gctaagttgt ttcaacggga tccaggcggt acggccagag ctgtctttca
    49861 cctgggcata attggtgttg gcgtcagttt gtaataaggt cacttcctcg ccggcgttaa
    49921 ccgtgcccac gaggcgataa tgatctcccg gaccgctacg gacccaggta ttcagttcat
    49981 cagaaacata gcgcgtttct tcagcgtgtg agacggcagt ggcgctaagt gcgagtaaag
    50041 ttaatccgat caggcgtaat tttggcatca ggctgtcgtt attgtcatgg aaagtggaac
    50101 gatagtagtg gcatcagtgt cgctacgcaa agcattcgac atcaatcgga atcctctgtg
    50161 tcaggtcttg ccactgcttc aaacttttac gcccgtcaaa ttgcccgttg catggcaatt
    50221 tggcgcaaaa tactatctac tgacaaaaaa gatcgcatta agttcgccgt tcatcgtcac
    50281 ttatgtgaca taaccgataa gtaaaggcca tggctcagga aatcgaatta aagtttattg
    50341 ttaatcacag tgccgttgag gcgttgcgtg accatctcaa tacgctgggc ggcgagcacc
    50401 atgaccccgt gcagttgctg aatatttact acgaaacgcc ggataactgg ctgcgtgggc
    50461 acgatatggg cttacgtatt cgtggcgaaa acggtcgcta tgagatgacc atgaaagttg
    50521 caggaagagt gacaggcggc ttacaccagc gcccggaata taacgtggcg ttgagcgaac
    50581 cgacgctcga cctggcgcag ttaccgacgg aagtctggcc gaacggcgaa ttgcccgccg
    50641 atctcgcttc ccgcgtacaa ccgctgttca gcaccgattt ttatcgcgaa aaatggctgg
    50701 tggcggtcga tgacagccga attgaaatcg ctctcgatca gggggaggtg aaagcggggg
    50761 aatttgctga acctatctgt gagctggaac tggaactgct tagcggcgac acgcgcgcgg
    50821 tgctgaaact ggcgaaccaa ctggtatcgc aaaccggatt acgccagggc agcctgagca
    50881 aagcggcgcg tggctatcat ctggcgcagg gcaatccggc gcgtgaaatc aaaccgacca
    50941 ccattttgca tgttgcggca aaagccgatg tggaacaggg gctggaagcg gcgctcgagc
    51001 tggcgttagc gcaatggcag tatcatgaag aactgtgggt acgcggcaac gatgcggcga
    51061 aagaacaggt gctggcagcc attggtctgg tccgtcatac actgatgctg ttcggtggta
    51121 ttgtgccgcg caaagcgagc actcacttac gtgatttgct gactcaatgc gaagcgacca
    51181 tcgcttctgc ggtttctgcc gtgacggcgg tctactctac caaaacggca atggcgaagc
    51241 tggcgttgac cgaatggctg gtaagcaaag cctggcagcc gtttttagat gccaaagcgc
    51301 agagtaaaat gagcgattcc ttcaaacgct ttgccgatat ccatctttcc cgccatgccg
    51361 ctgaactgaa aagcgttttc tgccagccgt tgggcgatcg ctaccgtgac cagttgccac
    51421 gtctgacgcg tgatattgac tcaatactgt tgctggcggg ttactatgat cctgtcgtcg
    51481 cgcaagcctg gctggagaac tggcaggggc tgcgtcacgc tattgcgacc gggcaacgca
    51541 ttgaaattga acatttccgt aatgaggcaa acaatcagga accgttctgg ttgcacagcg
    51601 gaaaacgtta atcaggcaag gataaaatcg cttatgaagc cgctctcttc accgttacag
    51661 cagtactggc agaccgttgt tgagcggctg ccagagcctt tagccgagaa atcacttagc
    51721 gcacaggcga agtcagtact tactttcagt gattttgtgc aggacagcgt gattgcgcat
    51781 ccagagtggc tgacggaact ggaaagccaa tcgccgcagg ccgacgaatg gcagcattac
    51841 gcggcatggt tgcaggaggc gctcagcaat gtgagtgacg aagccgggtt aatgcgcgag
    51901 ctgcggctat tccggcggcg cattatggtg cgcatcgcct gggcgcaaac gctggctttg
    51961 gttactgaag agagcatatt gcagcagctc agccatctgg cggagacgct gattgtcgcc
    52021 gcgcgtgact ggctgtatga cgcttgctgc cgcgagtggg gaacgccgtg caatgcgcag
    52081 ggcgaagcgc aaccgctgct gattttaggc atgggtaagc tgggcggtgg ggaactgaat
    52141 ttctcctcag atatcgatct gatttttgcc tggccggaac atggttgtac gcagggcgga
    52201 cgccgggaac tggataacgc gcagtttttt acccgcatgg ggcagcggct gattaaagtg
    52261 ttggatcaac caacgcagga tggcttcgtc tatcgcgtgg atatgcggct gcgtccgttt
    52321 ggcgaaagtg gcccgctggt gctgagcttt gccgcgttgg aggattatta ccaggagcag
    52381 gggcgcgact gggagcgtta cgcgatggtc aaggcgcgga ttatgggcga tagcgaaggc
    52441 gtctatgcta acgagttgcg tgcgatgctg cgcccgtttg ttttccgtcg ttacatcgat
    52501 ttcagcgtga ttcagtcgct gcgcaacatg aaagggatga ttgcccgtga agtgcgtcga
    52561 cgtggtttga ccgacaatat caaactcggc gcaggcggca ttcgcgaaat tgaatttatc
    52621 gttcaggtgt tccagctcat tcgcggtggg cgcgaaccgt cgctgcagtc gcgctcttta
    52681 ctgccaacgc tcagcgtcat tgccgcgctg catctgcttt ctgaaaacga tgctgaacaa
    52741 ttgcgagtgg cgtatctgtt cctgcggcgt ctggaaaacc tgctgcaaag cattaacgac
    52801 gaacaaaccc agacgcttcc ttctgatgag cttaaccgtg cgcggctggc gtgggcgatg
    52861 gactttgctg actggccgca actgaccggg gcgctgaccg cacatatgac caatgtgcgc
    52921 cgggtgttta atgaattgat tggcgacgac gaaagtgaaa ctcaggaaga gtcgctgtcg
    52981 gaacagtggc gtgagctgtg gcaggatgcg ttgcaggaag atgacactac gccagtgctg
    53041 gcgcatctta gcgaggatga tcgcaaacag gtgctaacgc tgattgccga tttccgcaaa
    53101 gagctggata agcgcaccat cgggccgcga ggacgtcagg tgctcgacca tctgatgccg
    53161 catctgctaa gtgatgtctg tgcgcgtgaa gacgctgccg ttacgctgtc gcgcattacc
    53221 gccttgctgg tggggattgt tacccgcacc acctatttag aattgctcag tgaattcccc
    53281 gcggcgctta aacatttgat ttctctgtgt gccgcgtcgc cgatgattgc cagccaactg
    53341 gcgcgttatc cattattgct ggatgaattg ctcgatccaa acacccttta ccagccgacg
    53401 gcgaccgatg cctaccgcga tgagttgcgc cagtatttgc tgcgcgtgcc ggaagatgac
    53461 gaagagcaac agcttgaggc gctgcgtcag ttcaaacagg cgcagctgtt acgcatcgcc
    53521 gcagcggata tcgctggtac gctaccggtg atgaaagtga gcgatcactt aacctggctg
    53581 gcagaggcta tgatagatgc cgtcgttcag caggcgtggg ttcaaatggt ggcccgctac
    53641 ggtaagccaa atcacctcaa cgatcgcgaa ggccgtggtt ttgcggtggt cggctacggc
    53701 aagctgggcg gctgggagtt aggctacagt tccgatcttg acctgatctt cctccatgat
    53761 tgcccaatgg atgcgatgac tgacggcgag cgggaaatcg acgggcgaca gttttatctg
    53821 cgtctggcgc aacgcattat gcatctgttc agtacgcgta cctcttccgg cattttgtat
    53881 gaagtggatg cacggctacg cccgtccggg gccgcgggaa tgctggtgac atccgcagaa
    53941 gcatttgccg attatcagaa aaacgaggcc tggacgtggg aacatcaggc gctggtgcgt
    54001 gcgcgggtag tgtacggcga tccgcagctc accgcgcact ttgacgcggt gcgtcgcgag
    54061 attatgacgc tgccgcgtga aggtaaaact ctgcaaacgg aagtgcggga aatgcgcgag
    54121 aaaatgcgcg ctcatctcgg caataaacat cgcgatcgct ttgatatcaa agctgatgaa
    54181 ggcggaatta ccgatatcga atttattacc caatatctgg tgttgcgcta cgctcatgaa
    54241 aaaccgaagt taacgcgctg gtcagacaac gtgcgcattc tggaactact ggcgcaaaac
    54301 gacattatgg aagagcagga agcgatggcg ctgacccgtg cttatactac gctgcgcgat
    54361 gaacttcatc atctggcatt acaggaattg ccgggccatg tgccggagga ttgcttcacc
    54421 gcagagcgtg aactggtgcg ggcaagctgg cagaagtggc tggtggaaga atgaagtatg
    54481 gtattatcgc gcgcaaattt tgaatctctc aggagacagg aatgaaagta acgctgccag
    54541 agtttgaacg tgcaggagtg atggtggttg gtgatgtgat gctggatcgt tactggtacg
    54601 gtcccaccag ccgtatctcg ccggaagcgc cggtgcccgt ggttaaagtc aataccatcg
    54661 aagaacgtcc gggcggcgcg gctaacgtgg caatgaacat cgcttctctc ggtgccaatg
    54721 ctcgcctggt cgggctgacg ggcattgacg atgcggcgcg tgcgctgagt aaatctctgg
    54781 ccgacgtcaa cgtcaaatgc gacttcgttt ctgtaccgac gcatccgact atcaccaaat
    54841 tacgggtact ttcccgcaac caacagctga tccgcctgga ttttgaagaa ggtttcgaag
    54901 gtgttgatcc gcagccgctg cacgagcgga ttaatcaggc gctgagttcg attggcgcgc
    54961 tggtgctttc tgactacgcc aaaggtgcgc tggcaagcgt acagcagatg atccaactgg
    55021 cgcgtaaagc gggcgttccg gtgctgattg atccaaaagg taccgatttt gagcgctacc
    55081 gcggcgctac gctgttaacg ccgaatctct cggaatttga agctgttgtc ggtaaatgta
    55141 agaccgaaga agagattgtt gagcgcggca tgaaactgat tgccgattac gaactctcgg
    55201 ctctgttagt gacccgttcc gaacagggta tgtcgctgct gcaaccgggt aaagcgccgc
    55261 tgcatatgcc aacccaggcg caggaagtgt atgacgttac cggtgcgggc gacacggtga
    55321 ttggcgtcct ggcggcaacg ctggcagcgg gtaattcgct ggaagaagcc tgcttctttg
    55381 ccaatgcggc ggctggcgtg gtggtcggca aactgggaac ctccacggtt tcgccgatcg
    55441 agctggaaaa tgcagtgcgt gggcgcgcag ataccggctt tggtgtgatg accgaagagg
    55501 aactgaagct tgctgtagcg gcagcgcgta aacgcggcga aaaggtggta atgaccaacg
    55561 gtgtctttga catcctgcac gccgggcacg tctcctatct ggcaaatgct cgcaaactgg
    55621 gtgaccgtct gattgtcgct gtgaacagcg atgcctccac caaacggctg aaaggggatt
    55681 cccgccccgt taacccgctc gaacagcgta tgattgtgct gggcgcactg gaagcggtcg
    55741 actgggtggt gtcgtttgaa gaagacacgc cgcagcgctt gatcgccggg atcctgccag
    55801 atttactggt gaagggcggc gactataaac cagaagagat cgccgggagt aaagaagtct
    55861 gggccaacgg cggcgaagtg ctggtgctca acttcgaaga cggttgctcg accactaaca
    55921 tcatcaagaa gatccaacag gataaaaaag gctaaccgga aagcggttca cagatctcgc
    55981 ttcgtagcag gcaggcctat tgacgctgtt gttttttcaa cctaaagtaa aggaacaagg
    56041 gtaagggagg atttctcccc cctctgatca gttgttagta agtcgggaaa cttaacagta
    56101 acaacacaac cagtatgatg acgaacttca tcataaccct ttccttctat aaggcccctt
    56161 cctcgggagg ggctttcccg tttcagcccc ttgctgactc cccgattgtt gatgagcgcc
    56221 ggaccccgcg caacgctttc cctgaagcag ttttgccttc cccgagcggt cacgcaaaac
    56281 gctgcaacgt cctgagcatt tgcacctttt agcggaacgc agatataaaa aatccccggc
    56341 tttttgggcc agggatatgt gttaatgaaa ccttcttgac gctgattttt tttcaaccta
    56401 aagtaaagga acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct
    56461 gcgaaactta cgattaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc
    56521 ttctgtaagg ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg
    56581 attgttgatg agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg
    56641 agcggtcacg caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat
    56701 ataaaaaatc cccggctttt tgtgccaggg acgttcatag ctgaaaattt attctacgtt
    56761 ttcggcgacg ggagttgttg aggttaaggg tgaagtcaat gccgccagtg agccgcctga
    56821 aacgccaatt tcattgagca aggagtcaat cagcggtgcc tgtgtgcggt aagagagagc
    56881 ggctgataat gcttgttccg ccaggtttcc gccccccaca ttacccgtgt ttgcatcacc
    56941 cgcagcgctg ccacgattca gaccatcgac ctgaataatc ttgataccgt caatagattt
    57001 catcggctca acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa
    57061 tttaaggctg gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc
    57121 ttctgcttcc gccagaccct ttttacgtgt agcttcggct aactcaacga tagccgccgc
    57181 ctgcatctct gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt
    57241 ttctgcatcc tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt
    57301 ctggcgagtg gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc
    57361 acgcgcttct gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg
    57421 gttggcgatt tcggtgacct gctgttgttc gatctcttta atacgaactt cacgttcagc
    57481 ctcaaccttt cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg
    57541 ttcagccaga attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat
    57601 acgtgcattc tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa
    57661 cgcttcttgt tgttcaatct ccaacttgcg cgaaagtgca tcacggtttt tctcacgcac
    57721 cgcaacttct acatcctgtt caacttcgtt acgttcgcga cgacggcgct ccgtctcctg
    57781 agtcagtttg gttaaacctt cggcgtcaaa ggcattgttc ggattgaaat gttctttcga
    57841 ggtctggtta aagttggtaa gtgaaacgct ttccagctca agaccgtttt tcgacaggtc
    57901 ttctgcgaca gtattttgta ctccctgcac gaagttctcg cgggtatcct gtaactcatg
    57961 catggtcatt tgtgcagctg ttgcacggag ggcatcgaca aatttatctt caaccaacat
    58021 acgtaagtct tctggtgata gcgtgcgttg ccccaacgtc tgcgccgcag tggcgatgcc
    58081 ttctacagac ggttttaccc gtacaaagaa agcgactact acatcaacac gcatacgatc
    58141 tttcgtaatc aggctatcaa tggttgagcg gctgacttcc agcttcagag tattcatatt
    58201 gatggggatt atttcatgaa agatcggcat cacgattgcg ccaccgctca tcaccacttt
    58261 ttgcccacct aaaccagtac gaacaaaagc ttgctctgcc gaagcgcgac gatagagcct
    58321 ggcgaaaata attccaataa taaacagaat gcatacggca ataatcgcgg taaacatcca
    58381 ggagggcaca gaattaacaa tatcatccat tattaaattt ccttttgatt aataaattac
    58441 aatatttgtg gccagggatt gttttccgcc aaatatcgcg ttgcagaaag tcggcaaata
    58501 attaacactt tgtctccttt agtgaaaatt tttccttctt caggctccag tagtaaataa
    58561 tgtatttggc caaattgatc ggtgagtttt ccttcgcagg gattacctga agtggcttga
    58621 tgaccggtaa ttaatgccat gctaccaata tattcttctt ctgtaatagc tgaactatgg
    58681 tcgcgtggaa tccagggcgc aactatttta ccggtataat gcaccgcaat aatcgtaaac
    58741 agtaaactga caggaaccac gaacaggttc gacagtggtg attgccagac catgatgcag
    58801 gcatgctgca acaggatgcc aataagacca aaaaaaccag ccagcaagca gagaacgacg
    58861 agagctggca atcttccgat attgagataa tgaagtgcct ggctaatatg acctgtggtt
    58921 atggaatcat aatggtcaag atgtgcatca agagcaccgg agagcatgtg accgcagata
    58981 agcgctaaaa tttctaaaag gccgatcaac agtacaaagg aaatagcgaa aagataaggg
    59041 gtattatagt cggcgaataa aatcatatgt cactccaatg tccatttagt gacgcaatat
    59101 ttactttggg atagtttcct tgcttacatt tatctttgtt aataataaca catgttagag
    59161 aagttctgga agagaaagtt ttttaatatg gttttttatt tttcactaaa aagtgtgatc
    59221 ggggacaata tatttacgca cgttatgttt aaaggcacta cactgattgg ggaatactga
    59281 aatcagaaaa agcatatggc cttaaaaggt atgcgataac cggaggatgt gcttatggat
    59341 catagtctta attctttaaa taatttcgat ttcctggcgc gtagttttgc cagaatgcac
    59401 gcagaaggtc gcccggtcga tattcttgcc gttactggta acatggatga agaacataga
    59461 acctggtttt gcgcacgtta tgcctggtat tgtcaacaga tgatgcaggc aagagagctg
    59521 gagttagagc actgatataa cgggcctgat ggcccgtttt agtgtttgta ttagatgcta
    59581 gttataggtg attttgagga tgacgtcttt actaaacggt ccctcaacaa cagtctctgc
    59641 cggattcgca ctaagttctg ccctgaatgg tgcggtgacc gaaggtgcac tagacgatga
    59701 aaaagaagca aattgtgaga agcggttaaa ttccgttgct gttccatctt tatgaaatat
    59761 cttcaatagc agtccattac ccatatccag atggctataa tcactcaaaa tatccgtagt
    59821 gaaaaagctc cctaaaatat tgaattgctc agtacaggcc gcaccaggat cttttgtcat
    59881 gactacacta aacgtctcac taacgctggt atttttaatt gttcgggaat ttattttacc
    59941 aaagtcaaca acgccatcag ctggttccag tgcgacatta acaaagcagt caaggacatg
    60001 aatattattt aatccacgaa tcctgaaggt caaatttttg tctacttcta tagctcctgc
    60061 gccactcata cctttaaact ctagagcgat atattcatca ggtaacacca ggtttttatt
    60121 aactctattg gcattttttg ctaatttaat atagagttgt acgcgagctg gaaaggttac
    60181 tgataattga ggctcaggac ctccactaca gacagcgggg tttttatagt gattattaaa
    60241 ggtacccgct ccttttatat caatgcattc tggtgctgcg atagcattag gggcagtcga
    60301 atcataatca actccattgt atgttacgcc aaaaatatag gagttctgat tataaaagtc
    60361 ctgaccgcct ggtaaaaaca tcattatatt agtccaggct gatgggtatt cttctccagg
    60421 tgatggggca ggtaatgcat tattacagaa gacattcata aaatactcac tgctttgcca
    60481 gatcttatca ccttttttac cattactggg gatagaaatt cttggtacgt aaccaatgac
    60541 tacggtattt cccccttcat gttcagcata gcagtccatt gcccatacag gtaatgcact
    60601 aaaacctata ataaatataa ttacaattaa taagtagcgc atattattta tctcgtttgg
    60661 ctacggaaac attacaagag ttattaaccg tcgggcagtt tatttcataa aaacttaatc
    60721 caccaaaatc attgatataa ccaacctgaa aagctgacgg aacattttta aactgcgtat
    60781 ctgcatggct ataaggggtt accattaaac tttgcgtttg tggcaacatt gtgattccgt
    60841 ttgttccgat ataacccagc gtaacgtgat aaggcatctt attgttaaag cggatcgaac
    60901 catcatttct acggatgatt tcaacttttt cgtaagacat taccccatca tcttctaatt
    60961 caatagcttt tggtcgccag aatagtttca atgcgtgctg catagtgact tgcatcacat
    61021 tttttttatc tgtttttggc ggtatttctc gcacgttaaa atagaagagc gtttctctgt
    61081 cattaggcag ttgagcaatt ttttcctgac ttatcattct tatttgaatt tgctcactgg
    61141 gatttaaacg taccataggg ggaaccgtga caataaagtc acgtgattta ttgcctttcg
    61201 tatcttctat ccatgtttgc gccagatagg gtagattttt gctttgatta gtaagtttta
    61261 atgcttcccc tttatctgac tcattcatta tgatacgggt acggtccggg atgatggcac
    61321 cccaggcaat gctgctcatg caggcgagta atactcctgc tgcaatgaga ttctttgtgt
    61381 ttaagtagcg cattagtttc atcctgaatt aattttggct aatacacggt aaaataaccg
    61441 ttccggtggt taagttgctg ctattgggtg gttgaataaa acaggattta tcaccccagc
    61501 gtaaagtaag tttgctgtca tcctgaatgc cgttgaggta tacaaagccg ccatcagcaa
    61561 ccagtcctaa ttctttatgg ctggttttat cttttacgct aatccccagt ggtgggtgac
    61621 taccatctgc caggcgaata acccccatga tttgatagcc cttgcgggta tcaagtttgg
    61681 cgtagccaat agcaccttcg gttaaggtgg tggtgataac cgagttttcg atatccacat
    61741 tttctggaag atttcgggta tcaacgctta atgatgttgt tatgtaactg ctgactgatg
    61801 gaataactcc gatgccatac cgattagtta ccgcgcgttt attgttcaac ggaatatctt
    61861 ctgcaccatc tgcgtcgatc ataaaacgcg agtcagcact accactatag tcatggaaag
    61921 cggctccgtg acgagtcgcc gtgaatgaac cattccaact ggcattcaga gaataataac
    61981 tgtctcgctg tgatgtcccg gagagataga gacgaccgtt ctcggtatta tgctgataac
    62041 tggcgcgtag tgacgctttc atatctttga attcatcatt gtcgcctgaa gcagaaacgc
    62101 tccaggaatt atttcgatcg gaagagtcat accacgaagc agtatgcgaa atcttgttat
    62161 cctgatttcg ttgcatacca tagctcaatg ttctactggt tccccaggga atcgaaatat
    62221 ttaggtacag ttgatcctgg ttgtcttctt cccagtttat tcggctaaat gttagagaag
    62281 tggagacgtt ttttaatggc ccgatatcaa aatcgcggct aagtgaaaat gaataattca
    62341 cattactgct ggcgtcccag taagtattac gagccaaact tacgtatgta tttaacctta
    62401 atgattcaag atactggtta aacgtgacaa tataactctc tttttcattc tgataattcg
    62461 tgtaatggtt agtgtcattg atatattcat tcattgtcac aaagttttta tcagagaaac
    62521 ggtaaccagc gaaagtgagc tggctaccgg tagattcaaa acgttttgaa tagttagcgc
    62581 ggtagctata acccgtttct ttatcctgat tatgcaactg agcatcagat cgtgtgacat
    62641 caaaagataa tgaacccaat gagttaaggt taaagccaac gccggcagcc agagattgat
    62701 aatcacggtt tgttaaaact gaaccaccat acagggatac attgttcagc caaccccatg
    62761 agaattcacc agtccagaag aagggttgtt gtaaggaatc accaccaacg ctggtcggtt
    62821 tacccatagc agtggtgtag cgaatctgcc ctttacgcgt cagataagga atactattag
    62881 atgcaacttg ccattgcgtg acttgcccgt cctcttcttc aaccttgacg tccagttgcc
    62941 cctgtaatgt attgaataaa ttagtaatag caaatgggcc aggaggaaca ttttcttgat
    63001 aaataatacg gttgttctgt gagacagtga cctttgcatt cgtttgtgca acacccgtga
    63061 tttgcggtgc gtaaccacgt agatcaggcg gcaacatatt ctcatcgctt ttcaacgaaa
    63121 cgccagaaaa atggaaagag tcgaaaatag aggattcagt atcgtattga cctatagtta
    63181 gttttgcatt aattgctggg attgggcgaa acaaataaat acgcggaaaa tccaggttgt
    63241 tcgcctggct tttgccattg ttaaatgatt gatcgtactg gtaatcagta cgcaggcgcc
    63301 aggccccaag attaacccca gcctgcccat aggaacttat attttgtgaa ttatcgccct
    63361 gatgtggtgc ataatgagag gcatataaat tgtagtcaag aaatgcccca gcaataccat
    63421 gatcccaaag ttcaggaggc gtccagtttg catctttgta ttttaaccat gcctgaggtg
    63481 cagatatgga taattgcatt ttccctttat cgagataagt tgtaatttgt ttttcttttt
    63541 cgatagggta acaaccatcg atcaggtttt gctgagccac ttcaataatc tcatcagtaa
    63601 agcctaataa tgttaattgc tctggagtca gacaaactgc agtttggtta tcaattaata
    63661 cccattgcag gtccagatgg attggcgtcc ttttattatt aataagagcg tttaatgagt
    63721 atttacctac gggtaattgg ccatcagttt caaattggga aaggttaacg ttttcacgat
    63781 cttcggcttc gattaaatct ttgttgaatt caacggcata agctgacgca caacc
//
LOCUS       DAPGWD010000010        61386 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPGWD010000010 DAPGWD010000000
VERSION     DAPGWD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.010"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(72..956)
                     /gene="xapR"
                     /locus_tag="RHX88_000576"
     CDS             complement(72..956)
                     /gene="xapR"
                     /locus_tag="RHX88_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDV2229308.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(997..1155)
                     /locus_tag="RHX88_000577"
     CDS             complement(997..1155)
                     /locus_tag="RHX88_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HDV2229309.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(1208..2464)
                     /gene="xapB"
                     /locus_tag="RHX88_000578"
     CDS             complement(1208..2464)
                     /gene="xapB"
                     /locus_tag="RHX88_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDV2229310.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(2524..3357)
                     /gene="xapA"
                     /locus_tag="RHX88_000579"
     CDS             complement(2524..3357)
                     /gene="xapA"
                     /locus_tag="RHX88_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDV2229311.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            3606..4370
                     /locus_tag="RHX88_000580"
     CDS             3606..4370
                     /locus_tag="RHX88_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229312.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKLGSEQHYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(4409..5335)
                     /locus_tag="RHX88_000581"
     CDS             complement(4409..5335)
                     /locus_tag="RHX88_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2229313.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            5425..6423
                     /locus_tag="RHX88_000582"
     CDS             5425..6423
                     /locus_tag="RHX88_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDV2229314.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQLQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(6420..6638)
                     /locus_tag="RHX88_000583"
     CDS             complement(6420..6638)
                     /locus_tag="RHX88_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDV2229315.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(6640..8655)
                     /gene="ligA"
                     /locus_tag="RHX88_000584"
     CDS             complement(6640..8655)
                     /gene="ligA"
                     /locus_tag="RHX88_000584"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDV2229316.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(8726..9712)
                     /gene="zipA"
                     /locus_tag="RHX88_000585"
     CDS             complement(8726..9712)
                     /gene="zipA"
                     /locus_tag="RHX88_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDV2229317.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9942..10703
                     /gene="cysZ"
                     /locus_tag="RHX88_000586"
     CDS             9942..10703
                     /gene="cysZ"
                     /locus_tag="RHX88_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDV2229318.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10888..11859
                     /gene="cysK"
                     /locus_tag="RHX88_000587"
     CDS             10888..11859
                     /gene="cysK"
                     /locus_tag="RHX88_000587"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDV2229319.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            12243..12500
                     /gene="ptsH"
                     /locus_tag="RHX88_000588"
     CDS             12243..12500
                     /gene="ptsH"
                     /locus_tag="RHX88_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDV2229320.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            12545..14272
                     /gene="ptsI"
                     /locus_tag="RHX88_000589"
     CDS             12545..14272
                     /gene="ptsI"
                     /locus_tag="RHX88_000589"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDV2229321.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            14313..14822
                     /gene="crr"
                     /locus_tag="RHX88_000590"
     CDS             14313..14822
                     /gene="crr"
                     /locus_tag="RHX88_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDV2229322.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(14864..15715)
                     /gene="pdxK"
                     /locus_tag="RHX88_000591"
                     /gene_synonym="thiJ"
     CDS             complement(14864..15715)
                     /gene="pdxK"
                     /locus_tag="RHX88_000591"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDV2229323.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            15820..16188
                     /locus_tag="RHX88_000592"
     CDS             15820..16188
                     /locus_tag="RHX88_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDV2229324.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(16191..17102)
                     /gene="cysM"
                     /locus_tag="RHX88_000593"
     CDS             complement(16191..17102)
                     /gene="cysM"
                     /locus_tag="RHX88_000593"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDV2229325.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(17236..18333)
                     /gene="cysA"
                     /locus_tag="RHX88_000594"
     CDS             complement(17236..18333)
                     /gene="cysA"
                     /locus_tag="RHX88_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDV2229326.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(18323..19198)
                     /gene="cysW"
                     /locus_tag="RHX88_000595"
     CDS             complement(18323..19198)
                     /gene="cysW"
                     /locus_tag="RHX88_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDV2229327.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(19198..20031)
                     /gene="cysT"
                     /locus_tag="RHX88_000596"
     CDS             complement(19198..20031)
                     /gene="cysT"
                     /locus_tag="RHX88_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDV2229328.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(20031..21047)
                     /gene="cysP"
                     /locus_tag="RHX88_000597"
     CDS             complement(20031..21047)
                     /gene="cysP"
                     /locus_tag="RHX88_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDV2229329.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(21218..22009)
                     /gene="ucpA"
                     /locus_tag="RHX88_000598"
     CDS             complement(21218..22009)
                     /gene="ucpA"
                     /locus_tag="RHX88_000598"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDV2229330.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(22138..22995)
                     /gene="murR"
                     /locus_tag="RHX88_000599"
     CDS             complement(22138..22995)
                     /gene="murR"
                     /locus_tag="RHX88_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDV2229331.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCSLFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            23159..24055
                     /gene="murQ"
                     /locus_tag="RHX88_000600"
     CDS             23159..24055
                     /gene="murQ"
                     /locus_tag="RHX88_000600"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDV2229332.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            24059..25483
                     /gene="murP"
                     /locus_tag="RHX88_000601"
     CDS             24059..25483
                     /gene="murP"
                     /locus_tag="RHX88_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDV2229333.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            25488..26792
                     /gene="pbp4b"
                     /locus_tag="RHX88_000602"
     CDS             25488..26792
                     /gene="pbp4b"
                     /locus_tag="RHX88_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDV2229334.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(26850..27749)
                     /gene="yfeX"
                     /locus_tag="RHX88_000603"
     CDS             complement(26850..27749)
                     /gene="yfeX"
                     /locus_tag="RHX88_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDV2229335.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(27845..28420)
                     /locus_tag="RHX88_000604"
     CDS             complement(27845..28420)
                     /locus_tag="RHX88_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDV2229336.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(28481..28930)
                     /locus_tag="RHX88_000605"
     CDS             complement(28481..28930)
                     /locus_tag="RHX88_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDV2229337.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(28917..29342)
                     /locus_tag="RHX88_000606"
     CDS             complement(28917..29342)
                     /locus_tag="RHX88_000606"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDV2229338.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            29556..30425
                     /gene="amiA"
                     /locus_tag="RHX88_000607"
     CDS             29556..30425
                     /gene="amiA"
                     /locus_tag="RHX88_000607"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDV2229339.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            30429..31328
                     /gene="hemF"
                     /locus_tag="RHX88_000608"
     CDS             30429..31328
                     /gene="hemF"
                     /locus_tag="RHX88_000608"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDV2229340.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(31334..32386)
                     /gene="eutR"
                     /locus_tag="RHX88_000609"
     CDS             complement(31334..32386)
                     /gene="eutR"
                     /locus_tag="RHX88_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDV2229341.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(32432..32932)
                     /gene="eutK"
                     /locus_tag="RHX88_000610"
     CDS             complement(32432..32932)
                     /gene="eutK"
                     /locus_tag="RHX88_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDV2229342.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(32945..33604)
                     /gene="eutL"
                     /locus_tag="RHX88_000611"
     CDS             complement(32945..33604)
                     /gene="eutL"
                     /locus_tag="RHX88_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDV2229343.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(33614..34501)
                     /gene="eutC"
                     /locus_tag="RHX88_000612"
     CDS             complement(33614..34501)
                     /gene="eutC"
                     /locus_tag="RHX88_000612"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDV2229344.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(34522..35883)
                     /gene="eutB"
                     /locus_tag="RHX88_000613"
     CDS             complement(34522..35883)
                     /gene="eutB"
                     /locus_tag="RHX88_000613"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDV2229345.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(35895..37298)
                     /gene="eutA"
                     /locus_tag="RHX88_000614"
     CDS             complement(35895..37298)
                     /gene="eutA"
                     /locus_tag="RHX88_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDV2229346.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(37295..38521)
                     /gene="eutH"
                     /locus_tag="RHX88_000615"
     CDS             complement(37295..38521)
                     /gene="eutH"
                     /locus_tag="RHX88_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDV2229347.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(38638..39825)
                     /gene="eutG"
                     /locus_tag="RHX88_000616"
     CDS             complement(38638..39825)
                     /gene="eutG"
                     /locus_tag="RHX88_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDV2229348.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDHD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(39815..40651)
                     /gene="eutJ"
                     /locus_tag="RHX88_000617"
     CDS             complement(39815..40651)
                     /gene="eutJ"
                     /locus_tag="RHX88_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDV2229349.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(40662..42065)
                     /locus_tag="RHX88_000618"
     CDS             complement(40662..42065)
                     /locus_tag="RHX88_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDV2229350.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(42077..42364)
                     /gene="eutN"
                     /locus_tag="RHX88_000619"
     CDS             complement(42077..42364)
                     /gene="eutN"
                     /locus_tag="RHX88_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDV2229351.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(42471..42764)
                     /gene="eutM"
                     /locus_tag="RHX88_000620"
     CDS             complement(42471..42764)
                     /gene="eutM"
                     /locus_tag="RHX88_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDV2229352.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(42803..43819)
                     /gene="pta"
                     /locus_tag="RHX88_000621"
     CDS             complement(42803..43819)
                     /gene="pta"
                     /locus_tag="RHX88_000621"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV2229353.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            complement(43816..44619)
                     /gene="eutT"
                     /locus_tag="RHX88_000622"
     CDS             complement(43816..44619)
                     /gene="eutT"
                     /locus_tag="RHX88_000622"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDV2229354.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(44616..45317)
                     /gene="eutQ"
                     /locus_tag="RHX88_000623"
     CDS             complement(44616..45317)
                     /gene="eutQ"
                     /locus_tag="RHX88_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDV2229355.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAKLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGQTMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(45292..45771)
                     /gene="eutP"
                     /locus_tag="RHX88_000624"
     CDS             complement(45292..45771)
                     /gene="eutP"
                     /locus_tag="RHX88_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDV2229356.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(45784..46119)
                     /gene="eutS"
                     /locus_tag="RHX88_000625"
     CDS             complement(45784..46119)
                     /gene="eutS"
                     /locus_tag="RHX88_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDV2229357.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(46412..48691)
                     /gene="maeB"
                     /locus_tag="RHX88_000626"
     CDS             complement(46412..48691)
                     /gene="maeB"
                     /locus_tag="RHX88_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDV2229358.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            48980..49930
                     /gene="tal"
                     /locus_tag="RHX88_000627"
     CDS             48980..49930
                     /gene="tal"
                     /locus_tag="RHX88_000627"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV2229359.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            49950..51953
                     /gene="tkt"
                     /locus_tag="RHX88_000628"
     CDS             49950..51953
                     /gene="tkt"
                     /locus_tag="RHX88_000628"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV2229360.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTAGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(52049..53092)
                     /locus_tag="RHX88_000629"
     CDS             complement(52049..53092)
                     /locus_tag="RHX88_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDV2229361.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNVLLDYGNWRMNGLRCSL
                     DPLRREANVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(53218..53793)
                     /gene="nudK"
                     /locus_tag="RHX88_000630"
     CDS             complement(53218..53793)
                     /gene="nudK"
                     /locus_tag="RHX88_000630"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDV2229362.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(53861..55840)
                     /gene="aegA"
                     /locus_tag="RHX88_000631"
     CDS             complement(53861..55840)
                     /gene="aegA"
                     /locus_tag="RHX88_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDV2229363.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            56046..57746
                     /gene="narQ"
                     /locus_tag="RHX88_000632"
     CDS             56046..57746
                     /gene="narQ"
                     /locus_tag="RHX88_000632"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDV2229364.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            57910..61023
                     /gene="acrD"
                     /locus_tag="RHX88_000633"
     CDS             57910..61023
                     /gene="acrD"
                     /locus_tag="RHX88_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDV2229365.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(61122..61181)
                     /gene="ypfM"
                     /locus_tag="RHX88_000634"
     CDS             complement(61122..61181)
                     /gene="ypfM"
                     /locus_tag="RHX88_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDV2229366.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        14403 a        16908 c        15643 g        14432 t
ORIGIN      
        1 tgcgatttgc gaaattgcct gatgcgcttc acttagcaga cgactatttc cggcaattcc
       61 tgtctcctca cctactgtgt caatgcagcc aacagcttaa ccatcgcggg cgtcacctgc
      121 tgtgtttcat aaacaatata taaatctgca gggatgcgct gtttgagcgg acggaaaatg
      181 acacctggcc agttcatttg tgcgtagctg tccgctatca atgtgatacc aatacccata
      241 ctgaccatgg cgagtaccgt ttgcggttca ttaacttcgc gaataacaac cggtgaaaat
      301 cccacctgct ggcaaactcg ctgcaaaaaa tcccagtcag tgtacacggg cggcattgta
      361 acaaaatact cgtcacgtag cgcttccagc ggaacggtgg aaaatgatga gagatgatgc
      421 tcttcaggca tcgccaccag aaacgccgat tcatgcaacc gtaagctggt aaaacctgtc
      481 ggtggttctg tcgccattcg ccagatcccg gcatcgagtt cacgtcgttc cagcagggcc
      541 atttgcatcg cgggcatctt ttcgcgaaaa agaacttcaa cgttaggatt ttccctgagg
      601 aatcgccgca taaccgggcg catccgtccc cacatagccg ttcccactac gccgagttca
      661 atccgccctg cttctccccg acctatttgt tcaacccgag ccaatacatt attagcattc
      721 accagcaatc gacgcgattc ttccatcaag attttgcccg cgtgtgtcag tacgacgctg
      781 cgcgaatggc gaataaaaag ctgcgtgccg agttgatttt ccagctcttt aatatgaata
      841 ctgagcggag gctgagacat atttaaacgc gctgctgcgc ggccaaaatg caactcttcc
      901 gctacggcaa gaaaataacg gagcaactta agatctgttc tgtatacgcg ttccataatt
      961 aagtgctcca tataataaat acatcgtatt aaattatcat ggcataaagt atttcaccac
     1021 cagatatccg acatacataa acacaatgga taatatacct ggtaatacat ttggtacagg
     1081 aataggcaag cgcacgattg taaacaaagc accaatgcca aaaccaacgc cggaagaaaa
     1141 taaaatatct ttcactaatt aacctttatc ataaaagcag ctctgaagag cagagctgcg
     1201 aatcctttta atgagccacc gctcgatgct ttatcttttc agggtcatga ttatatttaa
     1261 acccaaagaa aaatatcact gcgagaaaaa gagcatatcc tgcaaacacc agccagatag
     1321 tttgccagtc ttttacgcca tccaccgaaa agtaatctac tgccatgcca ctcagaatcg
     1381 agccaaccca tgcgccgaca ccatttacca tggtcataaa gagcccctgc gcgctggcac
     1441 gaatgctgga atcaacttcc tgttcgacaa ataccgaacc agaaatattg aagaaatcga
     1501 atgcacagcc ataaacaatc atcgacagca gcagcaaaat aaatccggtt gttgacggat
     1561 cgccataggc gaagaagcca aagcgcagcg tccaggccac catactcatc agcatgacgg
     1621 ctttaatgcc aaatcgcttt aaaaagaatg ggatagtcag tataaagccc acttctgcca
     1681 tctgtgaaac tgacagtaaa atggagggat atttcaccac aaaactgtca gcaaactccg
     1741 ggttacgggc gaaatcatgt aggaacggat taccaaaaac gttggtaatt tgcagtaccg
     1801 cacccagcat catggcaaag aggaaaaaga tggccatgcg tggattttta aacagcacga
     1861 aggcatccag acccagcttg ctggcaagcg atgtggtcgc ttttttctcc gcaaccggaa
     1921 tcttcggcaa agtcagcgca taagccgaca gcagcaatga cgcaccggac gcgatataca
     1981 gctgcagact actcaattcc agatgcagca ggcttactgc ccacatcgcg acaatgaacc
     2041 ccaccgtacc aaaaacgcga atgggcggga aagcggtcac cgggtcaagc cctgcctggg
     2101 caagacagga ataagagacg ctgttcgata acgcaatagt cggcataaac gccatcgcat
     2161 tgactaacat cacccaaaac atcatatccg gatcagttac ggatgccgca taaaaaagta
     2221 cgcccgcaca caccaggtga cacagcatgt atgcacgttc tgcgcgcagc catttgtctg
     2281 cgatgatccc cattatacca ggcataataa tcgcggcgat ccctttggaa ctgtaaacca
     2341 tgccaacatt agcgccggtg aaatgaaggg tattaatcat gtaagagccg agggtaacca
     2401 gccagctccc ccagataaaa tattgcaaaa aggacattac ctttaagcgc atcgcgatgc
     2461 tcataatcgt ttccttgtcg catcatttgg cctcacccgc gaggccgttt cttattgttt
     2521 ttctcaggca attttgcgca gaaagccaca aataaggttg atgaagttct gcttagagag
     2581 ctccgcagcc gccagcgttt gcgcatgtga caatttcaca tcgcttaaac cttccgccat
     2641 attggtaatc gcagagaccg caacgacttt aaggtcgcaa tggcgagctg aaataacctc
     2701 aggcaccaca gacataccaa caacatcccc accaataatt tgcatcatgc gaatttccgc
     2761 cgcagtctcg aaattcggcc ccggatacga gacgaacacg ccctccgtca gagggaaacc
     2821 ctcttctttc gccacttttt gtaacagtgc gcggtattcc gcatcgtagg catttgccag
     2881 tgagaagaag cgctctccaa aacgatcatc gttaagaccc accatcggcg taccgggcat
     2941 ggtgttgata tgatctttca atgcgaccag gctgcctgcc cccacttccg gacgcagcga
     3001 gcctgccgca ttggtgcaga acagtagctc gcagcccagc agcttaaagg tacgaattgc
     3061 gtcagtcata atggtcattc cacgaccttc gtagaaatgt ccgcgacctt tcatacatac
     3121 caccggtacg ccctggagat ggcccagcac taactccccc gcatgaccat gtaccgtact
     3181 taccgggaag cccggcagct tttcgtagga aattgcgaca gcgttctcaa tctgatcggc
     3241 cagcgcgccg agcccggaac ctaaaataaa ggccactcgt ggcgtgaaat caggtttata
     3301 agtcttgata atatctatgc aaaacagcgg gttatgagaa aattgaacct gagacataca
     3361 tatccttttt ctgtagggtg gaatctaacg cctttttata gcgaggttgt cccggctctt
     3421 accaatacac ttttctcacg cattcaatag cgattcgata tcgatggatt tatatctttt
     3481 ctctattaat ccacggtaaa aactgtattg gcgatattaa ttcctttgca ttaacaatat
     3541 gtcgttttta gcaaaccact ttattaagca atctactaat aacgatcggc atagggaatt
     3601 atgctatgaa aaaacatctt ttaactctga cactttcctc tatattagcg atacctgttg
     3661 tatctcatgc agaatttaaa ggcggttttg cagatatcgg cgtgcattat ctggactgga
     3721 ccagccggac taccgaaaaa tcgtcgacca aatcacacaa agatgatttt ggttatcttg
     3781 aatttgaagg tggcgctaac tttagttggg gagaaatgta cgggttcttc gactgggaaa
     3841 acttctataa cggtcgccat aataaactag gtagcgagca gcattatacc tttaaaaata
     3901 ccaaccgtat ttacctgggt gacaccggat ttaatctcta cttgcatgcg tatggcacct
     3961 acggttctgc gaatcgcgtg aatttccacg acgatatgtt cctgtacggt atcggctaca
     4021 atttcaccgg cagcggttgg tggttcaaac cgttctttgc taagcgttat acagatcaaa
     4081 cctattacac tggcgacaac ggctatgtcg ccggctgggt tgcaggttac aactttatgc
     4141 tgggcagcga gaaattcact cttaccaact ggaacgagta cgagtttgac cgtgacgcta
     4201 cctatgcagc gggtaatggc ggtaaagaag ggctgaatgg cgcagttgca ctgtggtgga
     4261 atgcaacatc acacattact acggggattc agtatcgata tgcggatgac aaactgggcg
     4321 aagattttta ccaggatgcg ataatctact ccatcaaatt taacttctga ttgattactg
     4381 ccggggctcc cggcagtatg aattcactct atatctgata caacggatcc ccttcccgac
     4441 cagccattag cgcattgccc tggtctcgta ccacgtccca taacgcttca gcggcggtgg
     4501 aaagtgaacg gttcttcctg cgcaccagca tcaactggcg ttcaacaacc ggcgtaatgc
     4561 gctttaccac cagtgggcta ccttcaggca atggtagcgc cagtgcggga agaatactga
     4621 tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg atgtccaatc tcctgcacaa
     4681 tattcgcctg aataccattg cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg
     4741 cgtaatcctg caacaccaat ttcgctccct gtaatgcctg ccagggtaca taatcttcga
     4801 cggccaaagc gctatcgcga tggcaaagca gaaagaaagg ctcggaaaga atcgcttcac
     4861 attgcagatc gcccactggg cctggatcga tgacgatgcc aaagtcgaca tctccctggc
     4921 gaatactttc catcacccac tgctgcggac gatcgtgcag gacaaactga atatctggat
     4981 agcggcgatg gctttcagca atgcattgcg gaataagatg cgcggaaatg gtctggctgg
     5041 cagcgacccg aacttttccg ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc
     5101 tgttcagttc atccagcagt cgctcaagac gcaaagccag ctgctgccct gcatctgtaa
     5161 gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc agtatgattt tccagttcct
     5221 tcacactgtg actcactgcc gactggctca ggccgatacg ctctcctgca cgactaaaac
     5281 ttttctcctg cgctactgtg acgaaaacct ttaattgttt taaagaataa ttcatctgtt
     5341 atcttcatga atggatgcaa taaatcaatt ttatttctca aactggaaga agcacaatag
     5401 aaccatcgat catctggagt ctttatgaaa ctttttcgta tcctcgatcc tttcacctta
     5461 accctgatca cggtggtgtt gctggcctct ttctttccgg ccagaggcga tttcgtcccc
     5521 ttctttgaaa atctgaccac cgcagcgatt gccctgctgt tctttatgca cggcgcgaag
     5581 ttgtcgcgtg aggcgattat tgctggcggt ggtcactggc gactgcattt gtgggtaatt
     5641 tgcagcacct tcgtgctgtt tccgattctg ggtgtactgt ttgcctggtg gaaaccggta
     5701 aatgtcgacc cgatgctcta ctccggtttt ctctacttgt gcattctccc ggctaccgtg
     5761 cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg tcgcggcggc ggtttgttct
     5821 gcgtcagcat ccagcctgct ggggattttc ctttcaccat tgctggttgg tctggtgatg
     5881 aatgttcacg gtgcaggggg cagccttgag caggtcggta aaattatgct gcaactgctg
     5941 ctgccgtttg tgttggggca tctttcccgg ccgtggattg gtgactgggt gtcgcgcaat
     6001 aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt tggtggttta tacagcgttc
     6061 agcgaagccg tcgttaatgg tatctggcat aaagttggtt ggggatcatt gctgtttatc
     6121 gtggtggtca gctgcgttct tctggctatc gtgattgtag ttaacgtctt tatggcacgc
     6181 cgactgggct tcaataaggc agatgaaatt actatcgtct tttgtggttc gaaaaagagt
     6241 ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca catcggtgat cggtatgatg
     6301 gtgctgcccc taatgatttt ccatcagctc caattgatgg tctgtgcggt gctggcgcgt
     6361 cgatacaaac gccagaccga acagttacag gcacagcagg aaagcagcgc cgataaagct
     6421 taaagcggac gcttcagggg ctggaccagt tgcgtcagcc cctcggtttt aatcagcagc
     6481 gtgatttgca ttagctcacc gagcttccct gccgggaatt catctttgcg ggcaaaccac
     6541 agcagatact cctccggcag gtcgattaag cgacgccctt tatatttgcc aaacggcatt
     6601 atcgtattgg ctatttcaat cagctgctct ttttccatct cagctaccca gcaaacgcag
     6661 catttccgct tcgtcgatga cttcaatgcc cagttcctgc gctttcgcca gtttagaacc
     6721 tgccgcttct ccggcaatca ccagatcggt tttcttcgac acgctgcccg cgactttcgc
     6781 gcccagttca accagtcgag ctttagcgtc atcacgcgac atctggctta agctgcccgt
     6841 aagcaccacg gttttaccag caaacgggct gtcaatctct tccgcgttga taacgatcgg
     6901 cgcaggccag tgaacacctt ccgccaacag ctcgctgatg acattgcggt tgctttcttc
     6961 ggcaaagaag ttgtgaacgt gggatgcaac gacaatgcca acatcaggca ccttttgcag
     7021 ctcttcaatc gaagcggctt ccagcgcttc cagcgtgccg aaatatgccg ccagacctgc
     7081 tgcggtggcc tcgccgactt cacggatgcc aagtgcatag aggaagcgag caaaggtggt
     7141 ttctttcgct ttttctagcg cgttaaccac gttttgtgcc gattttggcc ccatacgctc
     7201 cagtccggtc agtttgcctg cggtgagttt gaacagatct gccggagtat ggacatattc
     7261 tttttcaacc agctgatcga tgattttgtc gcccattccg tcaacatcca tcgcacggcg
     7321 ggaaacaaag tgtttcagcg actctttacg ctgcgctccg caaatcaggc caccggtaca
     7381 gcgggcaacc gcttcacctt ccacacgctc aacgtcagaa ccacataccg gacaatgcgt
     7441 cgggaataca acctcacggg tatcttccgg gcgttcagaa agcacgacgt taaccacctg
     7501 cgggatcacg tcgccagcgc ggcgaatcac cactttatcg ccaatgcgta aaccaagacg
     7561 ttcgatttca tccgcattgt gtaaggttgc gttactcacc agcacgccag caacatggac
     7621 aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc
     7681 acgcacaaag gtcatctgct cctgcgccgg gaatttaaac gctaccgccc agcgcggggc
     7741 acgcgcgaca aagccaagct gctcctgctg ttccagtgag ttgaccttaa tcaccacgcc
     7801 gtcgatatca aagcccagcg tcgggcgatc ttcttccact ttgtgataga acgccagcac
     7861 ttcttccgcc gattcacaaa gcgttacccg atcgctgacc ggcaaccccc actttttaaa
     7921 ttgcagtaaa cggccaagat gagtatccgg cagctcgcca ccttccagaa caccaacgcc
     7981 atagcagaaa aaagtgagcg gtcgcttcgc tgtaatacgc ggatcaagct gacgcagtga
     8041 accagctgcc gcattacgtg ggttagcaaa aactttcccg cccgtgcgtc gcgcatcttc
     8101 gttaatcttt tcgaaccccg cctgcggcag gaacacttca ccacgcactt ccagacgcgc
     8161 cgggatattc tctccgtgca gcttcagcgg aatagcgcga atagtacgca cattagacgt
     8221 gatatcttcc ccggtggtgc catcgccacg ggtcgcggca ctgactaaaa cgccattttc
     8281 atacagaata ctgacggcaa ggccatccag cttcagctca cagcaccagg tgactttctc
     8341 gttgtttttc agacggtcct gcacacgttt gttgaaagca agaaagcttt cttcatcaaa
     8401 aacgttatcc agtgacagca ttggtacttc atggcgtatc tggctgaaag ccgccagcgg
     8461 cgcagcgcct acacgctggg taggcgaatc aggcgtaatc agttctggat gtttggtttc
     8521 cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc
     8581 catcacatga taaagatatt catgatggcg aagcgtcgtt cgcagttctg tcagttgttg
     8641 ttcgattgat tccatatcgc accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa
     8701 gaggagttaa tttgccttaa gtgtatcagg cgttggcgtc tttgacttcg cggatgatgt
     8761 cctggtactc gcgcaatttc tgcggagtca tcatacggcg ctgatcgtca agcacgacac
     8821 cgcccacttc atcggcaata tgctgcgcag attgcagcat cagcttgaag ttctgcagct
     8881 cgtcaccgta agacggtacc tgcataaaga tggtgacacc cggagtagtg aaatccttca
     8941 tttcaggatc aaaggttccc ggtttcacca tattcgccag gctgaataac gccgggccgc
     9001 tgccatccgg gctaagatga cgatggtaaa tattcatatc gccaaaaatg aagcccgctt
     9061 gttgaatgct gttaagaagc agttcaccgt ttagctcgct accgtgatgc gccgcgacgt
     9121 tcatgataat caccgcttct ttgcgcttcg gtttatccat aactggagcc ggttccgcta
     9181 caggctcagg ctgtggtgcc gctacgggtt ctgcaggctg gaaagcctgt tgtgccggtt
     9241 gcggtgctga atgcacaggc tgcggcgctg gcgcgacctg tggcgaaact ggctgctgca
     9301 acggctgttc aggctgcggc tgataggcag gctgctgcac cggctgcgct ggacgcggag
     9361 catgttgcgg cggtacctgc gcttcaggcg gctgctggac cggttgacgc ggctgcgcag
     9421 acgcataagg cggttggtac tggtgttgcg gcgacggacg agcagcctca tgctcctgag
     9481 cgttagccgg ggcatgattc acgcggtgaa cacgaacctc accaacgccc tcatcatctt
     9541 cgacatcctc gtcataagaa tcgtcgtcac gttttgactt cattcgtttt aatggccgat
     9601 cgcggaacat agaagatcgt tctttacggc tggtccagaa accatgtacc agtaaagcga
     9661 ttatggcgat cgcgccaaca atgattaata tcagacgcaa atcctgcatc attatattct
     9721 ctgttgttct aacaccttgc caccacggca aacatttact cactaagagt atttgccgat
     9781 tacctcaagt gcaagtgcac tattaacttt cacagcacaa agatagatga aatcgtgctt
     9841 tttgctgttt tttcgaacat atcctaactg tccattgcgc aattacccgg taaaatacgc
     9901 agaattttcc tgggattggt caaaaggagc tcatcctgat tatggtttca tcattcacat
     9961 ctgccccacg cagcggtttt tactattttg cgcaaggctg gaagctcgtc tcgcaacctg
    10021 ggattcggcg tttcgttatt ttaccgctgc tggtcaatat tttgttgatg gggggcgcat
    10081 tctggtggct ctttacacag ctcgatgtct ggatcccgac tctcatgagt tacgttccgg
    10141 actggctaca atggctgagt tatctgttgt ggcctctggc tgtcatctct gtgctgttag
    10201 tgtttggcta tttcttctcc acgattgcta actggattgc cgctccgttt aacggtttat
    10261 tggctgaaca actggaagca cgattgactg gcgctacacc gccagatacc gggattttcg
    10321 gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg gcaaaaattt gcctggtatc
    10381 ttccgcgcgc aattgtatta ctaattcttt acttcatccc tggtattggg caaaccgtcg
    10441 cgccggtact gtggttcctg tttagcgcct ggatgttagc catccagtat tgcgattacc
    10501 ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa
    10561 tcaccaatat gcagtttggt gctttaacca gcctgtttac gatgatcccg ctgcttaatc
    10621 tgttcatcat gcccgttgcc gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg
    10681 ataaacacgc gatgtggcgg taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa
    10741 caggggtggc ttatgccgcc ccttattcca tcttgcatgt cattatttcc cttctgtata
    10801 tagatatgct aaatccttac ttccgcatat tctctgagcg ggtatgctac ctgttgtatc
    10861 ccaatttcat acagttaagg acaggccatg agtaagattt ttgaagataa ctcgctgact
    10921 atcggtcaca cgccgctggt tcgcctgaat cgcatcggta acggacgcat tctggcgaag
    10981 gtggaatctc gtaaccccag tttcagcgtt aagtgccgta tcggtgccaa catgatttgg
    11041 gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac tggttgaacc gaccagcggt
    11101 aataccggga ttgcactggc ctatgtagct gccgctcgcg gttacaaact caccctgacc
    11161 atgccagaaa ccatgagtat tgaacgccgc aagctgctga aagcgttagg tgcaaacctg
    11221 gtgctgacgg aaggtgctaa aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc
    11281 gccagcaatc cagagaaata cctgctgctg caacaattca gcaatccggc aaaccctgaa
    11341 attcacgaaa aaaccaccgg tccggagatc tgggaagata ccgacggtca ggttgatgta
    11401 tttattgctg gcgttgggac tggcggtacg ctgactggcg tcagccgcta cattaaaggc
    11461 accaaaggca agaccgatct tatctctgtc gccgttgagc caaccgattc tccagttatc
    11521 gcccaggcac tggctggtga agagattaaa cctggcccgc ataaaattca gggtattggc
    11581 gctggtttta tcccagctaa cctcgatctc aagctggtcg ataaagtcat tggcatcacc
    11641 aatgaagaag cgatttctac cgcacgtcgt ctgatggaag aagaaggtat tcttgcaggt
    11701 atctcttctg gagcagctgt tgctgcggcg ttgaaactac aagaagatga aagctttacc
    11761 aacaagaata ttgtggttat tctaccgtca tcgggtgagc gttatttaag caccgcattg
    11821 tttgccgatc tcttcactga gaaagaactg caacagtaat gccagcttgt taaaaatgcg
    11881 taaaaaagca cctttttagg tgcttttttg tggcctgctt caaactttcg cccctcctgg
    11941 cattgattca gcctgtcgga actggtattt aaccagacta attattttga tgcgcgaaat
    12001 taatcgttac aggaaaagcc aaagctgaat cgattttatg atttggttca attcttcctt
    12061 tagcggcata atgtttaatg acgtacgaaa cgtcagcggt caacacccgc cagcaatgga
    12121 ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa
    12181 agtcgaaccg ccaggctaga ctttagttcc acaacactaa acctataagt tggggaaata
    12241 caatgttcca gcaagaagtt accattaccg ctccgaacgg tctgcacacc cgccctgctg
    12301 cccagtttgt aaaagaagct aagggcttca cttctgaaat tactgtgact tccaacggca
    12361 aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct gggcctgact caaggtaccg
    12421 ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc ggttgaacat ctggttaaac
    12481 tgatggcgga actcgagtaa tttcccgggt tcttttaaaa atcagtcaca agtaaggtag
    12541 ggttatgatt tcaggcattt tagcatcccc gggtatcgct ttcggtaaag ctctgcttct
    12601 gaaagaagac gaaattgtca ttgaccggaa aaaaatttct gccgaccagg ttgatcagga
    12661 agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac
    12721 gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc tttgaagggc atattatgct
    12781 gctcgaagat gaggagctgg agcaggaaat catagccctg attaaagata agcacatgac
    12841 agctgacgca gctgctcatg aagttatcga aggtcaggct tctgccctgg aagagctgga
    12901 tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg
    12961 caacatcctg ggcctgaaga ttatcgacct gagcgccatt caggatgaag tcattctggt
    13021 tgccgctgac ctgacgccgt ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt
    13081 catcaccgac gcgggtggcc gtacttccca cacctctatc atggcgcgtt ctctggaact
    13141 gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg aaaaatgacg actatctgat
    13201 tctggatgcc gtaaataatc aggtttacgt caatccaacc aacgaagtta ttgataaaat
    13261 gcgcgctgtt caggagcaag tggcttctga aaaagcagag cttgctaaac tgaaagatct
    13321 gccagctatt acgctggacg gtcaccaggt agaagtgtgc gctaacattg gtacggttcg
    13381 tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt
    13441 cctgttcatg gaccgcgacg cgctgcccac tgaagaagaa cagtttgctg cttacaaagc
    13501 agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt accatggaca tcggcggcga
    13561 caaagagctg ccatacatga acttcccgaa agaagagaac ccgttcctcg gctggcgcgc
    13621 tatccgtatc gcgatggatc gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg
    13681 tgcctcggct ttcggtaaat tgcgcattat gttcccgatg atcatctctg ttgaagaagt
    13741 gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc
    13801 gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca ccggctgccg caacaattgc
    13861 acgtcattta gccaaagaag ttgatttctt tagtatcggc accaatgatt taacgcagta
    13921 cactctggca gttgaccgtg gtaatgatat gatttcacac ctttaccaac caatgtcacc
    13981 gtccgtgctg aacttgatca agcaagttat tgatgcttct catgctgaag gcaaatggac
    14041 tggcatgtgt ggtgagcttg ctggcgatga acgtgctaca cttctgttgc tggggatggg
    14101 tctggacgaa ttctctatga gcgccatttc tatcccgcgc attaagaaga ttatccgtaa
    14161 cacgaacttc gaagatgcga aggtgttagc agagcaggct cttgctcaac cgacaacgga
    14221 cgagttaatg acgctggtta acaagttcat tgaagaaaaa acaatctgct aatccacgag
    14281 atgcggccca atttactgct taggagaaga tcatgggttt gttcgataaa ctgaaatctc
    14341 tggtttccga cgacaagaag gataccggaa ctattgagat cattgctccg ctctctggcg
    14401 agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg
    14461 gtattgctat caaaccaacg ggtaacaaaa tggtcgcgcc tgtagacggt accattggta
    14521 aaatctttga aaccaatcac gcgttctcta tcgaatctga tagcggcgtt gaactgttcg
    14581 tccacttcgg tatcgacacc gttgaactga aaggcgaagg cttcaagcgt attgctgaag
    14641 aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt tgatctgccg ctactggaag
    14701 agaaagccaa gtctaccctg actccggttg ttatctccaa catggacgaa atcaaagaac
    14761 tgatcaaact gtccggtagc gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt
    14821 aattcttgcc gcagtgaaaa atggcgccca tcggcgccat tttttatgct tccgccagcg
    14881 gcggcaaaat caattcatcg ctctcatgct gctgggtgta gcgcatcact tccagtacgc
    14941 gcaaccccgc acggtgcacc gcatcgttta acgccttccc tttcagcaaa ccactgataa
    15001 gctgagcaca aaacaggtcg ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa
    15061 tgacattcac gctgtcggca ctgaccacca ccacctgcat ctcctgattt tcttcattac
    15121 cggaggcgct ggtaatcacc acccatttta atgtgtctga aagcagactt tttgcggcag
    15181 caatggcact gtcgagatcg cggcaatttt taccggtcag gatttccagc tcaaagatat
    15241 tgggggtaat tccctgcgcc agcggcagta aatattgtcg atacgcctcg ggaaggtcag
    15301 gtttgacata aattccgcta tcaatatcgc caatcaccgg atcgaccatg atcaataggt
    15361 caggatggtc tttgcgtagc gcagtcagcc actcggcaag gattttgatt tgcgatgccg
    15421 ttcccatata gcccgtggtt acagcgcgca gctggcgcag cgcatcacgc tcctgaagcg
    15481 cacgcaaata accgctaaac cattcatccg gaatcgcacc accgtagaaa gtgtcataat
    15541 gcggcgtatt gctcagcaat accgtcggca cggcaaaaac attcagaccg ttctgtttga
    15601 tagcaggcac ggcaatgctg ttgcccacgc tgccgtaaac cacctgcgac tgcacggcga
    15661 cgatatccgc ctgcagcgcc ctgctcttat cgttaaacaa caacaaacta ctcatttaat
    15721 tttttctcct tgccgatgat cctcatcgta ttccaaccga aactttacct gattctggca
    15781 gtcaaatcgg ctatcacaaa acaaggataa ggtaattcaa tgaagaaaat catttgtctg
    15841 gtcattacac tactaatgac actccccgct tacgcgaagt taactgccca tgaagaagcc
    15901 cgcatcaacg ccatgctgga gggattagca cagaaaaagg atttgatatt tgtgcgcaac
    15961 ggtgatgaac atacctgcga tgaagcggtt tctcatctgc gtctgaagct gggcaatacc
    16021 cgtaaccgca ttgacactgc cgagcagttt attgataagg ttgcttcgtc gtcatcgatt
    16081 accgggaagc cgtatattgt gaagatcccc ggtaagagcg atgagaacgc acagcctttt
    16141 ttacatgcgc taattgcgca gacggataaa acggtgcctg cgcaataatc ttaaatcccc
    16201 gccccctggc taaaatgctc ttccccaaac accccggtgg aaaggtagcg atcgccacga
    16261 tcgcagatga tcgctaccac caccgcacca gggttagctt ttgccacccg cagtgctccg
    16321 gcaaccgcgc cgccggagct gacgccacag aatattcctt cccgcactgc cagttcgcgc
    16381 atggtgtttt ctgcatcgcg ctgatgaata tccagcacct catccaccag agaagcgttg
    16441 aaaatccccg gcagatattc cgcaggccag cggcgaatgc cgggaatact gctgccttct
    16501 tccggctgca ggccaacaat cgttactggt ttggattgtt cgcgcataaa gcgtgaaacg
    16561 ccggtgatag tgccggttgt tcccatgctg gagacaaaat gggtaatgcg cccgccggtt
    16621 tgctgccaga tttccggccc ggtagtggtg taatgggcgt aagggttatc gggattatta
    16681 aactgatcga gcagctttcc ttcgccacga ttcgccatct ccagcgccag atcgcgcgca
    16741 ccttccatgc cctgctcttt ggtgacaaga atcagttccg caccataagc acgcatcgcc
    16801 gcacggcgtt cctggctcat gttgtcgggc atcagcaatt tcatgcgata gcctttcagc
    16861 gcggcaatca ttgccagcgc aatgccggtg ttaccactgg tggcttcgat taagacatca
    16921 cccggtttaa tttccccgcg cttttccgcc tcgacgatca tcgaaagtgc cgcacgatct
    16981 ttcaccgaac ctgccgggtt attgccttcc agttttaacc acacttcact gccgttatcc
    17041 ggccccattc gctgcaactt caccagaggc gtattgccta ttgtttgttc taatgtactc
    17101 acgatctcta tccatacgtg gtgttgcctg atgcgacgct tgcgcgtctt atcaggtcta
    17161 caggttacaa accttgccat taaaaagccc ggatcgcggg aagcgcctcc gggcgtttaa
    17221 cattcactca acctatcagg cgctttgtgc gagagcaagt tcctcatctc gggtttcgat
    17281 acgctcgtcg ccgttataca gccgcgcatg ttgcagacca acgaataaac gctcgccacg
    17341 ctgcggggca tcgtcgccat gcatcacgac cgtcagcggt tcgttgtacc accccagcgg
    17401 ctgcaccact aattgggtgt agtgaccttt cgggctggct tccagtacct gtaccggcag
    17461 cggcgaatcg agactggtac ggcggctgat atccacttcc cacgggcgca ggaagagatc
    17521 caccggaccc tgatacgcag gtgtgtagcc cagcggccag cgatgcgcgc caacatggaa
    17581 ctgcccgccg cgaatggttc cctgcaggcg gttcacttcg cccataaatt cgagtacaaa
    17641 acgggtcgct ggttcgcgcc atacctgatt cggcgcatca gcctgttcaa tattcccctg
    17701 actcatcacc actacacgat cggctacttc ggtcgcctct tcctggtcgt gggtcacgaa
    17761 cacgctggtg aatttcagct cttcatgcag ttgacgcagc cagcgacgca gctctttacg
    17821 cacctgcgca tccagcgcgc caaacggttc atcaagcagc agaatttgcg gttctacagc
    17881 cagcgcgcgc gccagcgcca cgcgctgttt ctggccgccg gaaagctgtg ccggataacg
    17941 atctgccaga tgagcaagct gcaccatctc cagcaatttt gtcactttcg ctttgatggc
    18001 tgcggcattc gggcgctcgc gacgtggcag caccgtcagg ccaaaagcga tattgtcgaa
    18061 caccgtcata tgacggaaca gcgcgtaatg ctggaacacg aaaccgacct tgcgatcgcg
    18121 cgcgtgcagg cggctgacgt cggtgccgtg gaagcgaata tgcccgctgg tttgatgctc
    18181 cagcccggcg ataatgcgca gcagcgtggt tttcccggaa ccggacggcc ccagcaacgc
    18241 gaccatctga cctgaaggaa tatccagtga gatatcgttc agcacctggg tgcgaccaaa
    18301 cgacttctta atattggcaa tctcaatgct catgatgttc ctcctgctgt gcgcgttttt
    18361 cctgattctc caggcgccac tgcaacatac tttttaaaaa cagggtgata atcgccatca
    18421 gcgttaacag tgccgcagcg gtaaaggagc cgacggtgtt gtagtcctgc tccagcaatt
    18481 caatctgtaa cggcagcgaa agtgtttcgc cgcgaatcga gccggaaacc accgacaccg
    18541 cgccaaactc gccaatcgcg cgggcgttgg tcagcactac gccataaagc agcgcccagc
    18601 ggatgttcgg caatgtgaca cgacggaaca tctgccagcc agacgcgcca agcaaaatcg
    18661 ccgcctcgtc ttcctgactg ccctggctta acatcaccgg caccagttcg cgcaccacaa
    18721 acggacacgt cacgaagatg gtgaccagca ccattcccgg ccaggagaac ataatttgca
    18781 gattatgttc atccagccaa ccgcccagcg ggccgttaga gccgtagaac agcagataca
    18841 ccagaccggc aaccaccggc gatacggcaa acggaatgtc cagtagcgtc agcagtaact
    18901 ggcgtccagg gaagttaaag cgcgtcacca gccaggccag cagaatgccg aacaccaggt
    18961 ttaccggtac ggcaatcagc gcgatcatca ccgtcagcca gatggcgtgt agcatgtccg
    19021 gatcggccag attctgtaaa accggcatca gccctttgct gaacgcctgt acgaagatat
    19081 aaatcatcgg caccagcagg atgaacgccg ataccagcat cccgatgcca atcagaaacc
    19141 atttgcccca gttaatcggg cgcgcgtcat aacgcttcaa ttgggtaact tccgccatta
    19201 atgacctacc acacgccgac caaagcgact ttgcagagtg ttaattgaga acagcagcag
    19261 cagagatgcc gcgaggatca ccgaagcaat cgcgctcgct gccgggtaat caaactcctg
    19321 taagcgcaca aaaatcatca gcgacgtcac ttccgtcttc cacgcgatat ttccggcgat
    19381 aaaaatcacc gcgccaaatt caccaagact acgggtaaac gacagcgcca cgcccgccac
    19441 cagcgccgga gaaagctccg gcagcaccac tttgcagaaa ctctgccagc gcgttgcacc
    19501 aagcgtttcc gccgcttctt catattccgg gcctaactct tccagcaccg gctgcacggt
    19561 acgtaccaca aacggaatgc tggtaaaggc catcgccacc gcaatcccca gccaggtata
    19621 ggtgactttg atatcaaact tcgccagcca ttcaccgtaa aaaccgttta cagaaaagag
    19681 cgaggctagc gttaaaccgg cgactgccgt tggcagcgca aagggtaaat ccatcagcgc
    19741 atcaagcagc gtgcggcctg ggaagcgata gcgggttagg atccacgcca tcagcagacc
    19801 gaaaacgccg ttaaaaatcg atgccacaaa cgctgacagc agcgttactt tgtaggccgc
    19861 tactacctgc gggttggtga tcacctccca gtactgcgcc cagctcatct gagccagctg
    19921 catcaccagc gcggagagcg gcagcagcaa aatcaggcac acaaacagca gactggtgcc
    19981 gaggcttaag gtaaagcccg gcagcacgcg tcttgatgaa actgcaaaca ttaattacgc
    20041 cccgccgcta acagcttgtc taactcgccg ccgctggtga agtgggtttt catcacttcc
    20101 ggccaggagc caaatttgtc ttccacgcgg aacagctcgg tctgcgggaa tttgtttttc
    20161 agtttgtcca tgacttccgg gttattcaca cggtagtaat agtcggtgat gatggtctgc
    20221 gcctgtgggc tgtagagcca gttcaggtag gcttttgcgg ctttttccgt accgttggcc
    20281 tgcacatttt tatcgaccca cgccaccggg aattccgcca gaatattggt tttcgggatc
    20341 accacttcaa agccctgtgc ttcatactgt ttacggatgt tgttcacttc cgattcaaag
    20401 ctaatcagca catcgcccag gccacgctcg gcaaaagtgg tggttgcgcc acgaccgccg
    20461 gtatcgaaca cttcaacgtt tttcaggaac tgggtcataa actgttcggt tttggctttg
    20521 tcaccaccgt cagctttatc cgctgcgccc catgccgcca gataggtata acgcgcgtta
    20581 cccgacgttt tcgggttcgg gaaaatcagc ttcacgtcgg agcgcaccag gtcgttccaa
    20641 tcgtggatat tcttcgggtt acccttacgc accaggaagc ccatggtgga gtagaacggc
    20701 gagctattat tcggcaggcg cgactgccag tcggccggga tcagcttgcc tttatcgtgc
    20761 aggatttgta cgtcggtcac ctggttataa gtgacaacgt cggcttttaa gccctgtaaa
    20821 atcgccagcg cctgttttga tgacccggca tgagattgtt ttatcgtcag tttgtcgccg
    20881 ccgttatctt ttgcccattg ttgctcaaac ggcggattca gggcggcaaa cagctcgcgg
    20941 gagacgtcat aagaactgtt cagcagttcc gttgcctgta catggcccgc cagcagcaga
    21001 gaagcgacca gcgcgagtga gttctttttc agtaagttaa cggccattgc gcacccttat
    21061 aaatttaatg actttctaat agccatcata tttataacgg acgtgaaagg agtaacggtt
    21121 ttatataccg tttggtgatt tggaagttga aaagggaata agaaccgggt ttaaaacgaa
    21181 atcctcccca cacatgcagg gaggaaacag aggtgaatca gataccgacg ctaaccgtct
    21241 ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc ggttaaatag ctggattcat
    21301 ccgatgcgag gaaggccgcc agttcgccga cttccagcgg atcggcgagg cgacgcatcg
    21361 ggattgcttt cgccatttca gtcagcaccg actctggatc ttccgggttc gactggcggg
    21421 caatgctttc cgccattggt gtgcgcacgt atcccgggca aatggcgtta acgcgaatac
    21481 cagactgcgc gtactccacc gccagcgatt ttgtcaggcc aacaatcgcc gctttcgtta
    21541 aggcgtacgc cgtttcgcca ggatcggcca ccatatcgcc agtgaccgaa gacatcatca
    21601 caatgcgacc atctttgcgg gcaatcatct ccggcagcac cgccttcgtg acgttccata
    21661 cgcctttaat attgatgtca atatggaaat cgcgatcgtc atcgctcata tcgaggaaac
    21721 tgcccagacg acaaacgcct gcgttattca ccaggatatc aatgcgccct tctttttcct
    21781 tcgcgcgttt gatagctgcg gctaccgacg ccgggtcacg cacatcggcg acaaccgccg
    21841 tacagcgatg accacgacca cacagttcgt ccgccagctt ttcgatctca ggggagatat
    21901 ccagcaagat taggtttgcg ccatgacgcg caaaagttct ggcaattcct tcgccaattc
    21961 cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag tttacccatt ttaatgctct
    22021 ccttgttaat acagagcgtt acactccatt ccctctgaaa atacaggata aacaaaatat
    22081 tgctgcggat cagctcacta attcaggaaa atctcaatcc agtcgctgct ttatgcatca
    22141 tttcaggcgt tgtgttagct cactgctgcg ctgaatcatt ttcaatgact ccacatcatt
    22201 gagttgcaca agaccaacaa acagtaaatc ggtaacagaa ttttgcgccg tgcgggtaga
    22261 catcgacgag ctacgccatt cggtttcacc ggaaacggta tcgagcgtga aatgcgccag
    22321 ccgccgcagc ggagaatcag ccagagaagt aatggcaatc accgttgccc cctgctttcg
    22381 tgccgcttct gcgcataaga caatctcttt tttactgccg ctgtaagaga tagcgatttg
    22441 tacatcgcct tttttcaacg cctgggaaac ggtggcttgt acgtgcgtgt cggcttcgca
    22501 ggccacgcga tagccaattt tcatcagttt gaacgacaga tcgcgcccca ccagcgccga
    22561 tccgcccagg cccgttatct ggataaacgg tgctttactg atgacctcaa tgattttttg
    22621 cagccgcgcg tagtcgaaca acgaacaggt ttgctccagc gccagctctt tttcgcgatt
    22681 caatttgcgg gcaatcactt ccagcgaatc atcactggta atcgaactgt gcaggtgcag
    22741 cgccgtggca ttcgttttct cccggctggc actgtattcg cctattaacg ccatacgcaa
    22801 ttcagtaaat ccttgcgcgc cgagtttttg ggcaaatttc actatgctcg actggctaat
    22861 gccgagctgt ttcgccatct gccgtgaaga aacggattgc agctcactca cgttggcctg
    22921 cagaaagtca gcgatttttt gctcgttttc ggtgaattca ctccccgcat tgcttatttt
    22981 cgtcaggtac aacatcttcc actctcctgg gctggcagca taaattccgc ttaagttctt
    23041 atgacgctct tcacactctg cgagtgtaga ctcaatgatt cctttaagtc tgtcaattcg
    23101 ggatatatga ttccatacat ctgcaattat ttgatcgtaa atagtaaggt caccaccgat
    23161 gcaactagaa aagatgatta ctgaaggctc gaacaccgcc tcggctgaaa ttgaccgcgt
    23221 atcgacgctg gaaatgtgcc ggattatcaa cgatgaagat aaaaccgtac cgcttgccgt
    23281 tgagcgcgtc ctgccggata tcgccgcggc gatcgatgtt atccacgccc aggtcagcgg
    23341 cggcggtcgt ctgatttatc tcggtgcggg aacatccggt cgtctgggga ttctggatgc
    23401 cagcgaatgt ccgcccacct acggcgtgaa accgggtctg gtggttggtt tgattgctgg
    23461 aggcgaatat gccattcagc acgcggtgga aggcgctgaa gatagccggg aaggtggtgt
    23521 taacgatctg aaaaatatta atttaacggc acaggatgtg gtggttggca ttgctgccag
    23581 cggtcgcacg ccgtatgtga ttgccggact ggaatacgca cgccagctcg gctgccgcac
    23641 agtgggaatt tcctgtaatc cggggagtgc cgtttccacc accgccgagt ttgccattac
    23701 accgattgta ggtgccgaag tggtcactgg ttcttcgcga atgaaagcag gcacagcgca
    23761 gaaactggtg ctcaatatgc tttccaccgg gctgatgatt aaatctggca aagtgttcgg
    23821 caacctgatg gtcgatgtgg tcgccaccaa cgaaaaactg catgtgcgcc aggtcaatat
    23881 cgttaaaaac gccaccggat gtaatgccga acaagcggaa gcggcgttaa ttgcctgcga
    23941 gcgcaactgt aaaacggcca ttgtgatggt gctgaaaaat ctcgatgccg cagaagctaa
    24001 aaaacgcctg gatcaacacg gcggctttat tcgtcaggtt ttagacaagg aataacccat
    24061 ggccaaagag atcagcagtg aacttctgaa caccattctt acccgtgtcg gcggaccggg
    24121 aaatatcgcc agttgtggta actgtatgac gcgcctgcgt ctgggtgtac atgacagttc
    24181 actggttgat cccaatatca aaacgctgga aggcgtgaag ggcgtcattt tgaccagcga
    24241 ccaggtgcag gtcgtttttg ggcctggtaa agcgcatcgt gccgcaaaag cgatgagcga
    24301 gctgctgggc gaggcaccgg tacaggatgc cgcagaaatc gccgcccaga acaaacgtca
    24361 gttaaaagcc aaacaaacct ccggcgtgca acaatttctc gccaaattcg ccaccatctt
    24421 cacgccgctg atccccggtt ttattgccgc cggtctgctg ctggggatag cgacgttaat
    24481 tgccacggtg atgcacgttc cggcagatgc tcagggaaca ctacccgatg cgctgaattt
    24541 tatgaaggtg tttagcaaag gcctgttcac cttcctggtg attctggtgg gctataacgc
    24601 cgcccaggca tttggcggca cgggcgtaaa tggcgcaatt atcgcggcgc tctttttgct
    24661 cggttataac cccgctgcaa ccacgggtta ctacgccgga tttcacgatt tctttggtct
    24721 gcccatcgat ccgcgcggca atattatcgg cgtgttgatt gccgcgtggg cctgcgcccg
    24781 catcgaaggc atggtgcgcc gctttatgcc ggacgatctc gacatgctgc tgacctcgtt
    24841 aatcaccctg ctgatcaccg ctacgcttgc gtacctgatc attatgccgc tgggtggctg
    24901 gttattcgaa ggtatgtcat ggctgtttat gcacctgaac agtaatccgt tcggttgtgc
    24961 ggttttagcc gggctgttcc tgatcgccgt ggtatttggc gtgcatcagg gctttattcc
    25021 tgtttacctc gcgttaatgg acagccaggg attcaacagc ttatttccca tcctttcaat
    25081 ggcaggcgcg ggccaggtgg gcgcggcgct ggcactctac tggcgggcgc aaccgcacag
    25141 tgcattacgc agtcaggtac gcggggcgat tattcccggt ctgctgggcg ttggtgaacc
    25201 gttgatttac ggcgtcaccc tgccccgcat gaaaccgttt attaccgcct gtttaggcgg
    25261 cgcagcgggc ggtttgttta tcggcctgat agcctggtgg ggtctgccga tgggcttaaa
    25321 cagcgccttt gggccgtctg gtctggtggc gctgccgctg atgacttccg cacaaggcat
    25381 ccttccggca atggcggttt atgctggcgg gattctggtg gcatgggttt gcgggtttat
    25441 tttcaccacg ctctttggct gccgtaacgt caatctggac tgatatcatg aaacggacaa
    25501 tgctctatct ttctctgctg gctgttagtt gtagcgtcag cgccgcaaaa taccctgttc
    25561 tgacagaaag ttcgccagag aaagcagggt tcaacgtcga acggcttaac cagatggatc
    25621 gctggattag ccagcaagtg gacgccggtt atcccggcgt aaacctgctg atcattaaag
    25681 ataatcagat tgtgtatcgc aaagcctggg gagcggcgaa aaagtacgat ggcagcgtgt
    25741 tgatggaaca gcctgtcaaa gccaccaccg ggacgctgta tgacctggcc tcaaacacca
    25801 aaatgtacgc cacgaacttc gccctgcaaa agctgatgag cgaaggcaaa ctgcatcctg
    25861 acgatcggat tgcgaaatat attccggggt ttgccgatag tccaaatgac accatcaaag
    25921 gcaaaaacac gctgcggatt tctgacctgc tgcatcacag cggcggtttc ccggcggacc
    25981 cacaatatcc gaataaagcg gtcgcgggcg cgttatattc ccaggataaa ggccagacgc
    26041 tggagatgat caagcgcacg ccgctggagt atcagcccgg cagcaagcat atttacagtg
    26101 atgtcgatta tatgctgctt ggatttatcg tcgagtcggt caccggtcag ccgcttgacc
    26161 gctatgttga agagtcgatt tatcgcccgc tcggcctgac acatacggta tttaacccgc
    26221 tgctgaaagg ctttaaaccg cagcaaattg ccgccacgga attgaacggc aatacccgcg
    26281 atggcgtgat ccatttcccg aatatccgca cctccactct ctggggtcag gtgcacgatg
    26341 aaaaagcctt ttattcgatg ggcggcgttt ccgggcacgc aggtttgttt tccaataccg
    26401 gcgatattgc ggtgttaatg caaacgatgc tgaacggcgg gggctatggc gatgtgcagc
    26461 tgttcagtgc ggaaacggtg aagatgttca ccaccagctc taaggaagat gccacttttg
    26521 gcctcggctg gcgcgtgaat ggtaatgcca ccatgacgcc gacgtttggc acgctggcaa
    26581 gcccgcaaac ctacggtcac actggctgga ccggaacggt gaccgttatc gatccggtga
    26641 atcatatggc gattgtgatg ttaagcaaca agccacattc gccggttgcc gatccgcaaa
    26701 agaatcccaa tatgttcgaa agcggtcagc tgccgattgc aacgtatggt tgggtagttg
    26761 atcaggtgta tgcggcgtta aagcaaaagt aataaaaagg ccagtcagta atgttactgc
    26821 ctggccttta atcaatgaat cagaaacgct tacagcgcca tcaacttgtc cagcgacggt
    26881 gcgaaataat agccgccggt taccggtttg gtgaaacgca acatcgcatc acgcttacca
    26941 tcggtatcgc caaacatgct cagcagttgc tgctcaatgt tatgcagacg cgcgcagtag
    27001 gcgcagaagt acagaccgtg agtgccactg gcagtgccgt acggcaggct ctggcgaaca
    27061 atcttcagcc ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga ggtttccgga
    27121 cgttcgtcgc cgtcgatctc ttcgttggcc tctttggtgc gcccgatcat catctcctga
    27181 tcgtgaacgc tcatccggtt gagctgcttc aggttgtgtt cccaacgctg gacaaacaca
    27241 tagctgccgc ccgcatccac gccgtctttg ataaccgcca cttcgcgacg cgtctcttca
    27301 cccgccgggt tttccgtacc gtcaacaaag ccgctcaggt cacgctcttc aacccaacgg
    27361 aagccgtgga tctcttcttt cacttcaatg cagtcaccaa aggcttccat cgccgcctgg
    27421 gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac atcgaactgg
    27481 gtggtcggcg caaggccttt accgtagccc ggaaaatctt tcagctcttc tgccccaacg
    27541 ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc aagatgcgcg
    27601 tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa atgttttact ggccgcacgc
    27661 agggcgtcaa cttccccttt cacgttggct tcgatccaaa tcgccgcgcg gcaatgttct
    27721 ggcaaaatgc cactctgaac ctgagacatt gttcctcctg aaaataataa tgccacgcaa
    27781 tccgtggcat tgttgagcgc tattttacgt gttttttcgc ggcggcggtt tgttcagacg
    27841 caaattaacg ccgccagata attttactga ctttccagtt tttcagggta tcgtccgaag
    27901 gcattaaccc ttcaggacca ctccattccc cgctgaactg gtagctaata tgttgactgc
    27961 cttcggcttt acattcgacg gcacgattat catcaccatc agctttttgg caattgccaa
    28021 atgctttgct gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg tcagcaggaa
    28081 tatcgctatc cagcacgtca atgcgactga tcgtgccttg atcgccgtta atcaccatcg
    28141 ccacgttgtc gcccttcatc acttcaaaaa agcgcaccac gttgccgttc gcggttttca
    28201 ttccgctgcg caggcgataa tcgccatcaa gcgcatcggc aatggcttgt tcttgcagtg
    28261 gtgtggacgc cgttaattca ccaacgccct gctcgctcac tttggtggac gacccaaacc
    28321 agttccacgg gttagcggca gaccagttaa ctgacgacat cgtggaacag ccggtcagca
    28381 tcaatggcat agcgcataac attaaacgca gcgatttcat gtcacttcct ttggttattc
    28441 aataacgttg cttggagtgc aaattcacca aaaagtgccg ttattctttt acttcattaa
    28501 aacaagcgcg taaacgtcga ttggtcagca gccagattaa cgccacaata tccgccacta
    28561 ccagcgccag accgataccg ctaacggatt caccgttcag ccacagccac ggttgccagc
    28621 aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag tacacgccag gtgcgaggaa
    28681 atgtagcccg ccgaccgctc aacaaaaacg ccagcaccgc cggaatgcct ggaatcagcc
    28741 ccagccagaa attatcgtga tcgggataaa acagatttag cagcgcagta ccctgctcgc
    28801 gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat agcacaagcc
    28861 agaagagcaa gggtaaacgc aggcgtccgt gcgcatcata atggacagga tggaactcag
    28921 tactcttcat cttcaatcaa acgcttaccc agactcagca cgtcggcgtg ttcatatccc
    28981 aggcgttcat acattccgag caccatgtcg ttatcttccg gcacattgat ctgaattttc
    29041 gggcagccac gagcaatcag ctttttctcc agccgattaa gcaacgcatt ggcaatccca
    29101 cgcccacgaa actctggatg cacgccaaga taataagcag acccgcgatg cccgtcataa
    29161 ccgcccatca ccgttccgac cacttcaccg tttacctcag cgaccaaaaa caaactgacg
    29221 tcatggttca tcttacgctc gatgtccatt tccggatcgt tccacggacg cagcaagtcg
    29281 caacgctccc aaagggtgat gacctcttcg aaatcttcct ggcgaaatac gcgtatctcc
    29341 atggtattcg ttaccttttt gcgggttaaa aggctgatta tggcgtgaac ggtcgaatta
    29401 gccaatatct gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt caactattga
    29461 aatgaaaagt aaaacaattc tcaacatcaa accgtcgtaa cggattacgc gatacgatat
    29521 aacatctgga actttattat tacaactcag gccgtatgag cacttttaaa ccactaaaaa
    29581 cactcacttc gcgccgccag gtgctgaaag ccggtttggc tgccctgacg ttgtcaggaa
    29641 tgtcgcaagc catcgccaaa gacgaacctt taaaaaccag caacggacac agcaagccga
    29701 aagccaaaaa atctggcggc aaacgtgtcg ttgttctcga tccaggccac ggcggaattg
    29761 ataccggagc gatcggacgc aacggttcga aagaaaaaca tgtggtgctg gcgattgcta
    29821 aaaacgtccg ttccattttg cgtaatcatg ggattgatgc gcgtttaacg cgttctggcg
    29881 atacgtttat cccactttac gatcgcgttg aaatcgccca taaacatggc gcagatctgt
    29941 ttatgtcaat tcatgccgat ggctttacca acccgaaagc tgccggtgct tcggtatttg
    30001 ccctctctaa ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa cgcgaaaacc
    30061 gcgccgatga agttgccggt aaaaaggcga ctgacaagga tcacctattg cagcaagtgc
    30121 tgtttgatct ggtgcaaacc gatactatta aaaacagcct gacgctcggc tcgcatattc
    30181 tgaaaaagat taagccggtg cataaactgc acagccgcaa caccgaacaa gccgcgtttg
    30241 tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga aacctcgttt atcaccaacc
    30301 cggaagaaga gcggctgtta ggcacggcgg cgtttcgtca gaaaatcgcc acagcgattg
    30361 ctgaaggcgt gatcagttat ttccactggt tcgacaacca gaaagcacat tcgaaaaagc
    30421 gataagttat gaaacccgac gcacaccagg ttaaacagtt tctgctcaac cttcaggata
    30481 cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat agttggcagc
    30541 gcgaagctgg cggcggcggg cgtagtcggg tgttgcgtaa tggtggtgtt ttcgaacagg
    30601 caggcgtcaa cttttctcac gtccacggcg aggcgatgcc agcttccgcc accgctcatc
    30661 gcccggaact tgccgggcgc agtttcgagg cgatgggcgt ttcactggta gtgcatccgc
    30721 ataacccgta tgttcccacc agccacgcga atgtgcgttt ttttattgcc gaaaaaccgg
    30781 gtgcagagcc cgtctggtgg tttggcggcg gcttcgattt aacccctttc tatggttttg
    30841 aagaagacgc cattcactgg caccgcaccg cccgtgacct gtgcctgcca tttggtgaag
    30901 acgtttatcc ccgttacaaa aagtggtgcg acgattactt ctacctcaaa catcgcaacg
    30961 aacagcgcgg tattggcggg ctgttctttg atgatctgaa cacgccagat ttcgaccact
    31021 gttttgcctt tatgcaggcg gtaggcaaag gctacaccga cgcttattta ccaattgtag
    31081 agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc taccgtcgcg
    31141 gtcgttatgt cgagttcaat ctggtctggg atcgcggcac tctgtttggc ctgcaaactg
    31201 gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg gagtatgatt
    31261 atcagccaaa agatggcagc ccagaagcgg cgttaagtga gtttattaag gtcagggatt
    31321 gggtgtaact ccctcacccc cattcccgca tccgctgatg caacgtcaac gacggcttct
    31381 cggcaaacag ctgctggtaa tccgtggcaa attgccccag atgccagaat ccccactgca
    31441 tggcggcgtc ttttaccgtt gtgctttgcg accacgggct tatcagttcg cggcgtacgg
    31501 cgttcaggcg aatgcgtttc agccacgcgt ttgggccaat gcctaaaata gcgtgaaacg
    31561 cgttttgtag cgtgcggcga ctgacatgca gttgattaca caagtccagc accgtcaccg
    31621 gctcagacat attttccagc acatattcac gggcgcggga aagtaatcta cggtaactct
    31681 gatgactgat gctttccgcc gtcaccattg gttgcgcgtc ttccagcatc gcccccatcg
    31741 ccattagcaa attatccccc agcacttttc gcactgcagg ctgatggaga ttttccgggt
    31801 tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca cagcgcggct ttatgctgct
    31861 ctttcacttc cagcgccgac tggctacgca gcatatgtaa tacccgatcc gggttatgca
    31921 aaaagttagc ctgccgggtg atgacatctt cagaaagcac cacgcccagg atcgtgtaat
    31981 catccggcgt gctcagttca aattcagtgc caccagggcg ggtggcgatt tccgcgcttc
    32041 ccagacattg cgaaccgata aatccctgct caccgcgcgt cgccggaatg ccaaaccaga
    32101 acgagttcgg ccagaccagg cacgactgac gcagcgccag accggtgtat tcacgaaaaa
    32161 cctgaatatc atcgagtaga atttccgtaa attcaccatg aaacttgccc ggatgcagct
    32221 gatcgtaaat ctgctgccag gcggtaatcg ttaaagcatg ttcatagaca tccgttgtct
    32281 gtcgttgatg aacattatcc acttcgacct tcggcgtgag cttcaggttt tcgggtaagg
    32341 gttcatgata aagatggtgc aaattggctg tacgggtctt tttcatgatg ttaatgccgg
    32401 gtgttgtagg acacccggca cctccgacag gttaatgggg cttgagacga taacgactac
    32461 tgcgtttacg taacgtcccg gcagaaaaga gctgttcgag cgcatttctg gctttttcca
    32521 gcggccagcc aaagtgggca gccacttctc ctgccgtcat tccctgacgt actgatgtta
    32581 acagcgccaa caactcgtca gcagattccg cgacgataac tggcgcgtcg ggttccctta
    32641 cgggttgctt cggctggcgg ttaaagccag taaccagcca ctgggtgtca tcgtctggcc
    32701 gaccgatctc cttgcggctg atgacacgcc cggtacgcat cgcggcagca caacctgcgt
    32761 ccagcgccgc acgacacgcc gccagatcgc cttccaccac cagcgttaag cgaccagggt
    32821 caagcacttc gtgactgagc agacgaacgt tagctgcttt gagcatggca tccgctgcat
    32881 ctatcgcggc gaccatgccg tccacttcca gcaatcccag tgcattgatc atcggcaacc
    32941 tccgttacgc acgctggatt ggattacgcg cgatttccag cactgcatcg gtaaaggcgt
    33001 tacaggctgc tttacacgcg gcctggctac cggttaaaaa tgctgccgag tagttggttt
    33061 cagacggtgg cgggacatag gtcaccagct gtacgtcggc agatttcaac gctgcatcaa
    33121 tgccataggt cgcttccagc ggcggtgcca ccagatacgc catcggatcg ccaagcgtga
    33181 tcccggcggt tgatgagaga taagaaccgg tacgcgaaac cacatgcgcc aggaaggcgg
    33241 tgttttctgc gtcattggcc cactggaatg ctgcaccatt ttcaatatgt gcaaccatcg
    33301 catccagacc ggcacgcact tccgccgggt ttggcccacc aagcataatc agcacttcac
    33361 cggcagtcgg tgacgggccg tgtgcagcgc ccgcatacag cgagcgacca tataccactt
    33421 ccaccatcgc ctgcttggtc gcttcatcag cggcaatgta ggtgacgtca tcggaatcag
    33481 cagaaatcag cccgaggcta cgaatatgcg gcggcaattt tagttcacgc gcaaattcag
    33541 cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa tctaaagctg
    33601 gcatgatgcc tccttatcgg gtcatgttga tgccggatgc tttctgctcc agcatacgtt
    33661 tcgccaaatc cacaatgacg gctgcggctt caaccggcgg cgtgccgccc tggtgaatgt
    33721 tagagataca ggtgcgatcg gcctcaacgg tggtcgccat acgcggcgag taaacggcgt
    33781 agcaggagag gctttctgac tgccctaacc ccggacgttc ccccaccagc aggatcacca
    33841 ctttcgcgcc gaggatctca ccgatctgat cttcaatctt cacgcgacca taacgaacaa
    33901 agaatggcgt gccgactttc agcccggcct gtttcaggcc cgccatcagc ggcggcagga
    33961 tctcttcgta gttcacggtg atggcatcag ttgacaggcc atcagaaata acgacctgta
    34021 cgtccggatt agcaacacac tgtgctttca gcgcttcaac agcttctgca cacaggcggc
    34081 ggcccatatc cgggcgagtc aggtagagat ttttgtcgct aatttcagag cgtacttcca
    34141 gcaagccctg cgctttcacc cactcttccg gcacttcttt cagtacggta tctttcgaac
    34201 gagagtgatc ggccaggaaa cgcagcagcg cctgggtacg tggacgcgga ccggcacgac
    34261 cggtacaaac gcgggccacg gtgctgcggc gcagttctgt taatacgtct gcgcgatgcg
    34321 ggttttcaac accaatccac gcttttgctt cagcggaacc taaatccagc gcacagcttt
    34381 ccgaggtcac cggtgccgca cagttggtgg tggcgcactt tgcttctgac ggcgcggggg
    34441 ccgtttgtcc cattgacgcc atcacgctgc gtacaatttc ttcaatctgt ttttgatcca
    34501 tgatgtgtta tctccgcgtc atcagaagaa cagtgacgga tcgcccgccc gtttggtcag
    34561 gcgaccgttt gccataatgc ccatgctttc cagccagcgt tcaaactccg gtgacggacg
    34621 caggttgagt aactgacgca cagtggcggt atcgtggaat gcggtggtct ggtagttgag
    34681 catgatgtca tcacccagcg gcatccccat gatgtagttg cagcctgcgg tggcgagcag
    34741 gatcatcagg ttttcgttga ggttctggtc agcgtcagcg tggttggtgt aacagcagtc
    34801 acagcccata gagatgccgc tcagcttgcc cataaagtga tcttctaagc cagcacggat
    34861 aatctggcgg tcgttgtaga gatactccgg cccaataaag ccgaccacgg tgttgacgat
    34921 aaacggatcg taatgacgcg ccagcccata gttacgtgct tccatcgtca cctggtcagc
    34981 gccgaagtta gcgccagcgg atagcgcaga gccttgtccg gtttcgaagt agaggcagtt
    35041 ttccccggcg atacggttga actctgcacc cactgcgcgc gcttcgtcga gcatcgccag
    35101 ttccacgcca aactctttca gccctttttc gctgccacag atactctgga aaatcagccc
    35161 gcctggtgcg ccgcgacgaa tcgcttcgat ctgggtggtg acgtgcgcca gtacgcagcc
    35221 ctgagttggg atgttgaatt tgtcgatcac gccataaatg gtatccagca cgcggcttaa
    35281 gttttccacg tcgtctgtca ccgggttaac gccgatcacc gcatcgccca ctccgaagga
    35341 aagcccttcg tagatttgcg cggcgatact ttgcacgtcg tcacgggtgt catttggctg
    35401 caaacgggcg ctaaaggtgc ccggaatacc gatggtggta ttggcctttt tgattaccgg
    35461 cattttcttc gcgccgtaga tcaggtccgc gttggagcaa atcttcgcta ccgccgcgac
    35521 cacttccgag gtcagccctt tgcgggtaaa ggcaatgtcg tccacgctgg tttcatcgct
    35581 cagcacatac tcacgcagtt cgctgatgct ccagttttta atctggttgt aggccgtttc
    35641 gttaacatcg tcctgaatca gccgcgtcac gcagtcatct tcataggcaa tcaccggatt
    35701 attgcggatg tccgctacgg tcatttccga caacacctgc tttgccgcca cgcgctcctg
    35761 tgaacttgcc gctgcaacgc ctgccagcac atcccccgaa cgcagttcgt tggctttagc
    35821 cagcacctct tttacatcct taaactgata tacattgccg aacaatgtgg tctttagttt
    35881 cataagtcgt tccctcagga aggaaatgcg agtgatttca ccgtcaccgg cacaaccgat
    35941 ccgccaaaaa gaggcgtacc aatgtcgata tagtcccccg cgcggacaat cacttcgtca
    36001 atgactgcca acgggagttg ttgtagctgt gggcgcaaca acatgcccag agctttacca
    36061 aagtcctgcc cggccaccac cagcagggga tgcggattcg gaaaacgcgc gacgaaatcg
    36121 accagcgcgt tgatgaccgt cagtaccgcg gcgtaacgca caggcagcga ggcgggaagc
    36181 gccagcacgt acgcgtcagt tttgggatca agatccagct gaatcagcgc ctgttgccag
    36241 gcactcacca gatccgtttc atcaattggg atcgccaccg gcaaattgcg cagcggcagt
    36301 tgtacgccct ccagccagat tgtgctgcca gagagcgaaa gggtatgtgc gcccgcgcca
    36361 atcaccgtgg cgcgtacggt ttgcgccgga aactgcacat tcatctcacg caggcgcgga
    36421 tggtcatgca gcgccgttgc cagcagcggg ccaatatcgg caaaacagaa cggatcggcg
    36481 ggctggtggc gataacattc gcccacgccg ccagaaagcg taatgatttc gggcgtaaca
    36541 cctgcgggca gcaaaccggt ttgcattaat gcctgcgcga gcggcgaaag cgttccgtca
    36601 atcacttcga caatcagctc tgccatccgc cgggtaacct gcaccagctg cgcgccggtc
    36661 agcgaacggg cgtcggtgcc tgcaccgaag cactcatcca caatcatctg ccccggttta
    36721 tgagcgtaaa ccacgcgccc ctggctgtcg gtttccagca ggcgaccgcc gacgttgagg
    36781 caggcagtgc cgctgatttt tccggcatcg aacagggcgt agttcgcggt gccaccgccg
    36841 atgtcgatat tcagtacccg acacagccgt tgttcagaaa gggtttgcgc cccggcaccg
    36901 tgaccggcga tcacggattc gaggtgcggc ccggcgctgg caacgacaaa atcgcccagc
    36961 gactgagaga gcgccatcac cgccgggcga gcattgcggg ttttcgcgct ttcaccggtg
    37021 atgatgatgg caccagaatc aacgctttcc ggcgcaatac ccgcagcctg atattgctcg
    37081 agtattaagg ttttcagttc cgcttctttt aaaccgccct gtttatcgac aggggtaaag
    37141 aacaccggac tttgccagct aatttcgcgt ttaatgaatt cgtagcgcgg cacctgcgac
    37201 accgccgcac ggttaaccag ctccaggtgg gagaaaatca cctgggtggt ggtggtgccg
    37261 atatcgatac cgacgctcaa tagctggcga gtgttcacga ttgtgcctcc gcttcggttt
    37321 tagtcgcggt cgcgtcttct tttggcacca gcatcatcgc cacgccaatc gccgttacgc
    37381 cgccgatcaa cttgccgaca atcatcggga agatcatggc gttcatgttg gcagcggcga
    37441 agcctaagtg gtcgcccagg gcgaaagcag cggaaacggc gaaggcgcag ttgatgactt
    37501 tgccgcgggt atccatctgc ttcatcatgc cgaacatcgg gatgttgttg gcaagcgttg
    37561 ccaccatgcc ggctgccgcg atgttgttca tattcagtac tttaccgacg ctcatcagcg
    37621 gtttttcaaa ccagcgagtc agcagcagca ccattggata cgcccctaac agcacgcagg
    37681 agatagaacc gataacttca atggcgcgca tcacttcacc gggtttatcg ccaggggcca
    37741 taaagatagg atccagaccg gggatcagtt cccagccaag caggaatttc actaccgcag
    37801 cggcaagacc gagggtgatc aatgcaacga ggaatttggc gaagatctgg aagccgttga
    37861 tcattttttc cgggatgaat ttcagcccca gcgccaccag aatcgcaaca atgatcaccg
    37921 ggatcatgtt catcaggatc agggcgaaag tgaattccac cggctgaccg ttgatctgca
    37981 caccggagta catagcaacc agaccaccag caatacagcc aatcggaatg gtcacaatgc
    38041 ccgccagcac gccgagcgcc agataacgac gatcagaagg ttcgataatg ccgagcgcca
    38101 ccggaatgga aaacacaatc gttggcccca tcatcgaccc gagaattaac ccagagtata
    38161 gccacgctgc tacgtctccg cccgccagct ctttggcgag gaagaagccg cccatatcgc
    38221 acgccagcag cgttccggcg aacattgacg ggtttgcgcc gagcatttcg taaaccggga
    38281 taattaccgg cccgagtacg tgagccagta ccggtgccag cgcggtcata ccgaccatcg
    38341 ccagacccag agcgcccatt gccataaagc cttcttcgaa ctgaccgcca gatccttcga
    38401 tacttttacc gaacttaccg aggaaacgag cagaaccgcc gaactgcgac aggatcctgt
    38461 ctacggcagc tatcagcata aagaacatca tgatgtacat gatgatttcg ttaattccca
    38521 tcgcctttac tccctgttag ttgttattta ttggcggatg cggcgtaaac gccttatccg
    38581 ccctacatgt gcaatcccga aggccggatc agacgcggca agcgtcgcat ccggcattta
    38641 ttgcgccgct gcgtacaagc cgacaatctg ctccaggctg gcggtacgcg ggttgctgcg
    38701 cagacaaata tcttcctgcg cggcctgcgc ccatgcaccg taatgcgcag atgtcgcgcc
    38761 aacatcgccc agtcgtttac caatcccaac ttccgcaatc agctcactta ccgcgttaat
    38821 agcgtcatga tcgtcggatt ttttagttcg cagtgcccga ccaatctgac taaagcgttc
    38881 acgacaaacc atccggttaa attccatcac tgttggcagc aacatggcgt tcgccagacc
    38941 gtgcggaata tgcagcgccg cgcccggctg atgcgccatc gcgtggcaca acccaagacc
    39001 cgcactggaa aacgccattc ccgccataca tgaagccagc aacatgctct cgcgcgcggc
    39061 aaggtcgtga ccgtagccca ccgctttcgg cagcgatttg ccaatcatcg caatggcacc
    39121 aatcgccaga ctgtcggtaa acggtgtagc gttcagggcg ctgtatgctt caatggcatg
    39181 ggttaacgca tcaatgccgg tcatcgccgt gacatgcgac ggcacacctt cggtcaatgc
    39241 ggcgtcgagg atcgccacat ccggcatcag cgaggcatgg gctaacacct gcttgcgccc
    39301 gctcaccgcg tcgataatca ccgttacatt ggtggtttca gagccggttc cggcggtagt
    39361 tggaatggca atcagcggca agcgcggttg cagaacgctg gtttctgaca tctctgccag
    39421 cgtgctatcc gggttcgtca ccagcaacgc cacggctttc gccgcatcca gcaccgagcc
    39481 gccgccaaat gcgatcaccc cgtcacaacc tgactcacgc aactgcgcca cggctgcaca
    39541 cacgtcggta atgcacggtt cgcccaccgg acatggccag agcgtcatgg cgatgccttt
    39601 aacagccagg ctgcgcgtca gcccggcggt catccctgcc tgatgcaaaa agctgtctgc
    39661 catcacgaac agatgtttca gcccacgcgt ttgcgcttgc tgtccgcaac tgctcaccga
    39721 gcccggaccg caaagcgtca ccggtggaac gctaaatgtt tttacccgtt gcagattcag
    39781 ggtatcgaac gcctgaaaga gcgcggtctg caattcattt tgcatatagc ccctccgctt
    39841 tctctcgccc actactggcg atcgccagcg gggtcataaa caaactgtgc tgcggtaaat
    39901 gcacttgtaa cgccgggaat tgtttgcgaa acagttccgc cacgcccggt tgcatacagg
    39961 agccgcccgc cagccataaa tcagtaatgc cctgcccttc aatatgccgg gcgacaatgt
    40021 ccgccatttt ttcgtacacc ggtttcaccg cgggccaaat ctcgtcacca tgaccgcgct
    40081 tgtactgctc cgcctcttcc agcgaaatac ggcgatttcc ggcgagagtc agagagatgt
    40141 gatgtccgcc ggtggcttca tccgccgagt acgtcacttt gccctttttc acgatggcaa
    40201 tgccggtagt gccgccgccg atatccacta caccggcgtt gtccagttgc agcagatccg
    40261 caactgcggt tggctcatcc agcacatggc tcacttccag tccagcagac tccagcacgt
    40321 tgatggaaat acgcgggtcc gtacctggag gaaatgaagt cgccgcatgg ctaaaacggc
    40381 gaccgaattg ctgctcgagc gtgtcgagat ggcgacgaac aatggtgaca gcgccgaaga
    40441 aatcccagac gatgccgtcg cggaccacat cggcccagtc gaggcatacc gccaccggct
    40501 gaccgtcgcg gtcaacaacc atcgacacca catcgcaggt gcccagatcc acccccagcc
    40561 acagcggaga ttccgtcgcg gcaggcgtct ggttacacag cgttgccgcc gtttgcagac
    40621 gtggggtgag ccattgttcg tcgtgcgcca tcggttactc cttaaacaat gcgaaacgca
    40681 tcgactaata cacagcgacg cagacggaca aacgtccgcg cgctggttac cccttcaccg
    40741 gttggcgtgg tgatggtcat ggtggtccag ccttccccgc ccagccccag cccggcaatg
    40801 cacggtccgt tcttaacgaa aatgctggta tcaatggcgt tcgccatctg gttcatgttt
    40861 tcgatattgc gcgagtgcat tgccgccgtg tggtggcaac cgccctccag tttcaccgcc
    40921 agcgcaatgg catccgccac gttggcgacg cgcacgacgg gcagcaccgg catcatcagt
    40981 tcggtcacgg caaacggatg ttctgcagtg gtttccacaa acagcaaacg cgtttcttgc
    41041 ggcacgttca ggccgattgc cgccgcgatt ttcgccgcat cgcgaccaac ccagtcacgg
    41101 ctgacggtgc cttttccgcg ctcgtcgata tttttcagca acaccggttg cagctgctgc
    41161 gcctgttctg cggtcagttt caccgcgtgc tggccttcca tcagacgcat cagttcatcg
    41221 gctacgctat caacaacaat cagtaccttt tcgtcggcac aaatgatgtt gttatcgaaa
    41281 gaagcgcctt tgacgatgga ctgagcggca cgggcgaggt cggcggtttc atccaccact
    41341 accggcgggt tgccagcgcc tgcggcaatc agacgtttat tggtgtgttt acgcgccgct
    41401 tctactaccg cttcgccgcc ggttaccacc agcaggccga tacccggaaa cttgaacaag
    41461 cgttgcgcgg tttcgatatc cggatttgcc acagtaacca gtaagttttc cggcccacct
    41521 gcggcaacaa tcgcctggtt gagcagcgta atcgcccgct gggagacttt tttcgccgcc
    41581 ggatgcgggg caaaaatgac gctgttgccc gcggcaatca ggctgatggc gttgttaatt
    41641 acggttgccg ccgggttagt ggaaggcgtc accgaagcca ccacgcccca gggtgcgttt
    41701 tcaattaggg tcaggccgtt gtcgccagtc agcacttgcg gagagaggca ctcaacgcct
    41761 ggtgtgccgc gcgcctgagc gacgtttttt gcaaatttat cttcaacgcg ccccatgccg
    41821 gtttcactga cggcaagttc cgctaaatct ctggcgtgtt tttcgcctgc ttcacgaatg
    41881 gcagcaatgg ctaactggcg cattgccacg ctttttaacc cttgctgggc gactttggct
    41941 gccgcaacgg cgtcatccag ggacgcgaaa acgcccatct catgaacggc ggcgggcggc
    42001 gtgtcactgc tttgcatttt cagcagtacc gctttcacca cctgttcaat atcctgttga
    42061 ttcatgatgt tctgccttat ttgtggaaaa ttacctgacc gccagacacc acctcatcga
    42121 caatgccaat cacgcacaga tcgaccggtg acgtttcgct tttatgcgcc tggcgggcgg
    42181 aactgccact caccagtaac acccactccc cggttcccgc gccaatattg tcgatggcga
    42241 cggcgcattg cccgtcagga ttaccttgtg gatcaatcat ttccaccatc agcaatttgt
    42301 catgcgccag tccgtgatgg cgtacggtac aaacaatttg tccagtgacg actgccagtt
    42361 tcatacccgc ctccgtggcg tatttcaggt aaaagctccc cctaccctcc gcagaaggta
    42421 aaatgaaaaa ggagagagcg tgacgcccga atcgacgtca cacagggtga ttacaggttg
    42481 ctgctatcgc ctttcaggcc gatcgggaag acttcttcca ggtcgccgtg cggacgcggg
    42541 ataacgtgta cagataccag ctcgccgata cgctgtgccg ctgcggcacc tgcatcggtc
    42601 gcggctttac aggctgcaac atcaccacga accatggcag tacacaggcc gccgccaatc
    42661 tgcttaacac caaccagctt gacgcgcgca gctttcacca tcgcgtcaga agcctcaatc
    42721 agtgcaacca ggccccgggt ttcgatcatt cctaatgctt ccattgtgct ttcctcttta
    42781 tcagggtcca gaacgggacc gttcattcaa ccagtgtttg taaactgctt tcgcggttca
    42841 cttttgtctg acgcggcaca gctgccacca gcgccagctc gataatttcc tgcacgctac
    42901 aaccacgaga gagatcgtgc atcggcgcgg caagtccttg tatcagtggc ccgacggcac
    42961 gatatccgcc gagtcgttgt gcgattttgt aaccaatatt tccggcttcc agcgacggaa
    43021 aaaccatcac attggccttg ccctgtagcg ggctggcagg cgctttttgc gccgccactt
    43081 ccggcacgaa ggcggcgtca aactgtaact cgccatcaac caccagcttt ggtgcgcgct
    43141 cacggacgat ttctgtcgcc tgctggacgt tggcaacgca ggggtgacgg gcgctaccgt
    43201 tactggaaaa cgacagcatc gccacgcgcg gctcttctcc ggtgatggcg cgccaggttt
    43261 cggcactggc aagcgcgata tccgccagct gcgccgccgt cggctgtggc accacgctgc
    43321 aatcggcaaa gcccaacgcc gggccgctgt actgtggcaa catcaggaaa atggaagaga
    43381 gcgttttaca gcctggctgc aagccgataa tgcgtaatcc ggcacgcagc acatttgccg
    43441 tggaagagag gttgcccgcg atacagacat ccgctttacc ggcgctgacc attgctgcgg
    43501 cgaacatcag agggtcggta agtttttcca gcgcatccgg cggcgttttt tcgcccgcgc
    43561 gggccagcca gcgatgagca aattcttccc gcattgcgag gttgccatgc ggatcaatca
    43621 cctgtagccc gtccatcgcc acgccgtgac tgagcgcaaa ctgacgaagt tcaaacggat
    43681 tggcgaccag aatgggcgtt gccagacctt gttgatgtaa atattgcgcg gctttcagca
    43741 cacgttggtc taacgcatcc ggaaaaacca ctctggcggg cgctcgcaac gccagttcac
    43801 gacaacgttc aataatcatt gcgtttcccc cagtcgttgt tggatctgct tcaccgttag
    43861 cggctgcttc gttacgctca ggatcatcat cacgtagacc gtgctcgaaa ggcggttcag
    43921 cgcctgcaaa atgtccgggc gtaatacttc aaagctgcgg gtgataaaca cctgcgccgc
    43981 tacggtttcg gtttcccgca ctttggtacg tagcagattg agcaacgccg catcgcgccc
    44041 gtggctggct tccggcacca gatgatcgtg gtcgagatag cgcagcggct gatgagaaag
    44101 ccgatgcaga tcttcgtcag aaagcccgac aatcgcctgg cagcccagcg gttcccccag
    44161 cgcatcggcg cgcataatgt tgccaagacg cgaacggata tccgctagcc acggctgcca
    44221 cggttccgcc agttcaattt gcagccacac tgccagcgca atggtgctgt cgagaaccgc
    44281 gcgaaaaccc agacgcggat cgcttttggc gaccattttt tccgccgaca ggtgagtcag
    44341 cgtatccggc ttttttgcca ccggctggcg acacagttcg cagcacgcct gcggatgttc
    44401 atcgctactg gtcagcccat gaacgggctg cggctgctgc tgttcatcgt caacaaacag
    44461 gcggccctgc tcgtcaataa acttgatgcg cagatggcgg ctttccagca actcccgggc
    44521 agagggcgtc aggcgactgt ccgcagggag atggatctct gcgccttcgc tgagcgtatg
    44581 gttcgctctt agccatgctt cggtgatgaa atctttcata gggattgcca gttagccggc
    44641 caggcgacat acaggaattt cacgctggat gtcgtaccaa attcgatgct ggagcctttc
    44701 gggataaaca tcacgtcgcc tgctttggcg atcatggttt ggccttcgtg gcgaacatgc
    44761 agctcccctt ccagcaccat gtcgatttcg tcgtagttca gggtccacgg gaagaatgcg
    44821 ttctcccact gcataaaacc ggcggccatg ctgctgccgt cgtctccggt cacaagatca
    44881 gtcaagccca cgcaatgcgg ctctgcgccg tcgaaacgac caaacttgac gctactgcca
    44941 tcaatcactt tgatgccgcc tttgccggtc accgatttga agctcggctg cattgcgccc
    45001 tgctccagcg actgtttttc cttcatcact ttttccatca gctttgcgac caggctttcg
    45061 gtaaactggc cttccggcag ctgggcaatg atggtttcgc ggatgcgctg gctttcggtt
    45121 ttgtcggctg gcacgctggc gggcacggac gccgttaccg ggatcgattc atcacattcg
    45181 gtgatggtaa aacccagtag atccgccact tcacgggctt ccggggtaat aatgctggcg
    45241 cgcagaacca cggacattgc ctgttcgccg cgcgcgtgtg cttcacgaat atcattcgct
    45301 gtgatgagtt ttttcacctg cttcctcctg tttggtgaga gatgccagat aatccaccag
    45361 ttgctgcacg ctttgcgggt cgtggctgtt tagctcgaaa atcggctctt cgaaacccgt
    45421 ctcaagcaat aactttcgcg ttgcggcgac gtcagcatct ggcatgtccg ttttactgat
    45481 gacggcgatt tgccgtttgc tgacgccaat atccagcaac ccggcaggca accgactttc
    45541 cgggtcattt gcgccatgga catagatcag catgtctaca tcctgtagcg tggtaattaa
    45601 ggcgtgatac cagcggggat ggctaaaata ttcacccggc gtatcaatat cgcccttatc
    45661 attaaattcc acggcctgtg tttttctggc gagggtataa ttcccctgca acgcattaaa
    45721 taacgttgtt tttcctgccc cgacgctacc cacaaacgca attcgtttca tgaatgcctc
    45781 gtattaactt ttggtcattt cacaaagcgt ataatttaat aagcgaccta aaccgctgac
    45841 ggtttgtgat aacgcttctt ctactgcgcc caccgagcca taaatcacca atgcaccgct
    45901 gaagcggtcg agaaaaccga tatgcacatc agcggctttc agcgctaaat caccggcgat
    45961 catcgccgtt tcgccgggag ttagcgtcat aatgccaatt gcgcccgcat ccggaacgcc
    46021 gattttcttc gccagttcct caccgggatg cgcgatgaga tgcgccagtg tgacctgttt
    46081 gccgggcaca aattcctgaa tgatgcgttc tttatccatg agtcgccgcc tgtgaaaaac
    46141 tttcgtgtat cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta
    46201 aaaggtgagg tagatcacta agaatggaga gatattcgct gtggtgcata aactttcata
    46261 tgcaacgcat gaatataaat aaaataatat cgagatatta tatagactgc tttttttatt
    46321 attcgcaaac tcagtaataa gctttactaa tatattgcag ccaaaccgct taccctgaat
    46381 attcagggta agcgtgagag ttaaaaaaga attacagcgg ttgggtttgc gcttcaacca
    46441 cggccagcgc caccatgttg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa
    46501 ccggtttcgc cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc
    46561 gcagtaagtt gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag
    46621 aacctttcaa agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat
    46681 cgccgtgcat ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg
    46741 cctgacgcat tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca
    46801 gagcaacgcg cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct
    46861 ccgccagctc ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac
    46921 cactcggcag cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa
    46981 agacattttt caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg
    47041 catcggcttc cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg
    47101 cccgctgcgc ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc
    47161 agtactcttt aaagcgcgga tcggattcgt tattgacgat ctcaaaatca acgcccgctt
    47221 tgatctgcaa gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga
    47281 tcggtttcgc cagtcccagc gttaccagtt cctgagtggc atgcagaacg cgcgcctctt
    47341 ccccttccgg cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca
    47401 taaacaggtt ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag
    47461 caatcggacg cgtcgccacg cccgactcca tcgccgcttt cgccaccgca ggagcgatct
    47521 taacgatcaa gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat
    47581 cctgatcgcc atacgctgaa gccaccactt cgctctgttc ggcgtgggcc agttctgcaa
    47641 tcgcgcgtac cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg
    47701 cgccacggaa gatgaacggg aagcaaagga cgttgttgac ctggtttgga tagtcagaac
    47761 gaccggtgca gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg
    47821 gttccgggtt cgccagcgcc aggatcattg gcgcacgagc cattttcttc accatttcct
    47881 gggtcagcac tttcgggccg gaacatccga ggaaaatatc cgcgccttca atcacatcat
    47941 cgagggtacg tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatgtttg
    48001 gctcacggcc ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca
    48061 gacccagcgc taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca
    48121 ccatccgcac gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag
    48181 tgctgatgat tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc
    48241 gcagtttctg ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgattccgc
    48301 cgaaggtcgg ttcgagcgca gcgacaactt caataaattt gtccgggtcg agttcatcaa
    48361 cttcaatgtc aaatacatca atcccggcga atttcttaaa cagaacgccc ttgccttcca
    48421 tcaccggttt gccagccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag
    48481 agatcaccgc caccaggtta ccgcgcgcgg tatatttgta ggcttttaac gggtcttttt
    48541 cgatttcaag acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg
    48601 ttgccagagg cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat
    48661 caagtgcact ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca
    48721 aataaagtgt gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg
    48781 agtctacaga ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat
    48841 cctataacac tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt
    48901 ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac
    48961 ttttcaagga gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt
    49021 ggcagacagc ggcgatattg agtccattcg ccattatcat ccccaggatg ccaccaccaa
    49081 tccttcgctg ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc
    49141 tatcgcctgg ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga
    49201 caaactggcg gtcaatttcg gtgctgaaat cctcaaaatc gtacccggtc gcgtgtcaac
    49261 agaagttgat gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct
    49321 ggtggacttg tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc
    49381 gacctgggaa ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct
    49441 gacgctgctg ttttcttttg cccaggcgcg ggcctgtgca gaagcgggcg tttttctgat
    49501 ttcgccgttt gtcgggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta
    49561 tgtggtggaa gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca
    49621 acaccactat gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc
    49681 cttaaccggc tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa
    49741 agtttcgcca gtggtacgta aattaatccc accttctcag acgttcccac gcccagctcc
    49801 catgagcgaa gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact
    49861 gtctgaaggc attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc
    49921 caaactataa accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc
    49981 gcgcactcag tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg
    50041 gcatggctga tattgccgaa gtgctgtgga acgattttct taaacataac cctaccgacc
    50101 caacctggta tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct
    50161 acagtttgct acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc
    50221 agttgcattc gaaaacccca ggccacccgg agattggcta tacggcaggc gttgaaacca
    50281 ccaccggccc gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcggaacgta
    50341 cattggcggc gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt
    50401 ttatgggcga cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca
    50461 cgctgggact gggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg
    50521 aaacagaagg ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg
    50581 tgatccatga aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc
    50641 aaagcgtgaa agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc
    50701 cgaataaagc aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc
    50761 tggcacggca aaaactgggc tggcaccatc cgccatttga gatccctaaa aagatttatc
    50821 acgcctggga tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg
    50881 ccgcctataa aaaggctcat ccgcaactgg cagaagagtt tacccgacgg atgagcggtg
    50941 gtttaccgaa ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc
    51001 cggcgaaaat cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc
    51061 tgcctgagtt gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag
    51121 gttctgtttc gctgaaggaa gatccagcgg gcaactacat tcactacggg gtgcgtgaat
    51181 ttggcatgac cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg
    51241 cgacgttcct gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga
    51301 aagcgcggca gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga
    51361 cgcaccaggc tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc
    51421 gaccgtgcga tcaggtggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg
    51481 gaccgacggc actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc
    51541 aggttaaaga gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata
    51601 ttattttgat tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag
    51661 caggagaagg tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc
    51721 aggatgagga atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg
    51781 aagcaggtat tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga
    51841 tgacgggtta cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg
    51901 ccgagaatat tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat
    51961 cgccggatgc tgattgccgg atgcgacgct gacgcgtctt atccggccta cagcgtctta
    52021 tccggcctac atgtccccgc aattttgttt aacgggtgat ccacaacgtg ggccaggcgt
    52081 ctggcccatg ccagttatcg caggtgggtt ctgcggcata acgcaccagg cgaaaacgct
    52141 gaccgtcaaa gcgccagcgc gcctgaatgc cacaatcgct taatccgcgc cctttcgcta
    52201 aggtcaccag ttcacgcgat ttctcatcaa atgttgcgtt catcagttcc agttcattcg
    52261 tctcctgacc gttgttgaac ggcaaacgca accgaaccgg gcgcgaggct agtggctttt
    52321 tacgcgacac aatccatgcc aaatcaatgg tgttataggc ccctgcctca cagctaatca
    52381 tcatcagcgc tttatcatca gtcagcgcag tgacattcgc ctcacgacgt aacggatcaa
    52441 gcgagcagcg cagaccattc atccgccagt ttccataatc cagcaaaacg ttgcgttctt
    52501 cgagtgagag tggcgtcggc gttgggttaa ccaccgcgac ctctttcagc gcaggcgcgg
    52561 gcggtacgct gagcggcggt tcgtcccctt tcttgatcca cgcggtttca ctgccaacgc
    52621 gcttttgctg agcatcaata aacaacaacg ccgcttttaa gccactcaga gaaatggtct
    52681 gattgccgtc gcgtaaggtg atcgccttcc cttcctgaat catctgcaaa aacgcggtga
    52741 tggttgccgt atcatcggtt actaataacc atggtgaaat ccgccacttg tcaccactta
    52801 acgccaacgg ctcaccatct aacagcagcc gtggcgctat ctctccttca gacgcatccg
    52861 gcgacttcaa cccgccgcgc tcaatacgta aaacggcatc ggtatgcgcc ccggcgctgc
    52921 ggctcagggt catcaccagt ccattatgat cgcccgtatt gcgcgccacg cagaagtttt
    52981 ggttattaca ggtgacctgc cagtcggaaa acgcccgttg cgctggtgcc gcccacacca
    53041 aagacgtcgg caacaaagcg aaaaaaaaga gaaggaaaat gcgatagcgc atggacggta
    53101 cgaccccaga agttcaaaca aacagcacaa gtcgtatctt cgttgcgcag gtagcgcggc
    53161 tcaatcagat ttgtcggatt gagccaataa taaacaacga aatattattg ttatttttca
    53221 gtccattaaa tgtgacgttt gcaaatagtt aagcaataac accgtcttac cgtcacgtat
    53281 ctcgccggtt ttgatcatct ccagcgcctg gctgaacggc agctcgagca cttcaatatc
    53341 ttcatcttca acaccgccac cggcgttagc gcgctgattg tcactgtatt cggcgataaa
    53401 aaagtggatt agctcagtca caccgccagg cgacatatac agttcaaata atttgcgcac
    53461 ttcgcccact tcatagcccg tctcttcaat cgcttcttta cgaatgcaca cttccggttc
    53521 gtcgttatcc agcagcccgg cgcaggtttc aatcagctgc ccgctttcat tgccattaac
    53581 ccaggtagcg acacggaact gacgaatcag aaccacggtc tttttcttcg cgttgtacag
    53641 gaggatcgtc gcaccattac cgcgatcgta aacttcacgt ttatggcgga taacttcgcc
    53701 atctttgcgc gtgagatcgt aagtaatgtt gtgcagggtg aaatagttat cggagagaat
    53761 tttgtcttta atgagggtga tttgttgcgt cataccgact ccacagcgcg aaatgaacaa
    53821 ttatattacg ctgtgaagtc gggtttgtct gcgcaggcta tcagtgagat ttgactgatt
    53881 ttacccccag ccagtcaata atcccctgtg ccgcatgacg tccttctgcc attgcggtaa
    53941 ccaccagatc cgcaccacgc acggcgtcgc caccggcgaa gattttcgga ttggtggtct
    54001 ggtaacggta ctggctttcc acatccgcga tgatgcggcc ccatttgtct accgttacac
    54061 cgtgcgactc cagccacggc atcccgtgtg gattgaagcc aaacgccata atcaccgcat
    54121 cggctggcat gacaaattca ctgccctcaa tcggtacagg tcgccgacgc ccctgggcat
    54181 ccggctcgcc aagacgcgtg cgcaggaaac gaatcccgca gacgtgacct tgttcattca
    54241 gctcaagcgc caccggctgg acgttaaatt cgaagttggc cccctcttcg cgggcgttcc
    54301 tcacctcttt cttcgagcct ggcatgttag cttcatcacg acgataagcg caggtgacgt
    54361 tactcgcgcc gtggcgcagc gcagtacgca cacagtccat cgcggtgtcg ccgccgccca
    54421 gtaccacgac gttaagtccg gcggtattga taaacggctc ttccggtagc tcttcgagcc
    54481 ccatcacctg tttagtgttg gcaatgagga acggcagcgc gtcataaacg cccggcgcat
    54541 cttcattggg taaacccgct ttcatggaac ggtaagtgcc tacgccaacg aagaccgcgt
    54601 cgtattgttc caaaagcgaa tccaaagaga catctttacc cacttcacaa ttgagttcga
    54661 agtgaatccc catcgcgctg aagatttccc gacggcgtgc cagcagggat ttatccagtt
    54721 tgaaagaagg aatgccgaaa gtgagcaagc caccgatttc tggatggcga tcgtacaccg
    54781 tcaccgccac gccattgcgg gtcagaacat ccgcacaggc cagccctgcc ggacctgccc
    54841 cgataatcgc cacccgcttg tccactttgg tgacatgact taagtcagga cgccaacctt
    54901 tcgccaacgc ctgatctgaa atatagcgtt caatgttgcc gatagttacc gcgccgtgct
    54961 catcgcgaat agtacaggca ccttcacaca aacggtcttg cggacaaacg cgtccggtaa
    55021 tttccggcag ggtgttggtc tggtgagaaa gctcgacggc ggcgtcgatg tttccggctt
    55081 tcaccagttc aatccactgc ggtatatggt tatgcagcgg gcaggtccat tcacaaacgc
    55141 tgtgctcgcc gcacttaagg cagcgcgagg cttcccgttg tgcctggtcg gcgcgaaatg
    55201 gcagataaat ttcatcaaaa ccggttttgc gcgcttcaat cgccagttta tccggctcgc
    55261 cacgcgcggg cgttgcctgc atttgttcga ctttactcat taccggcatt tcttgcgccg
    55321 cggtactggc atgccacggt tgatgttcct gacgcgcggt gcgcaagcgg cgggatttcg
    55381 ccatgccgga gagtgcgacg tcagtgacca gttgcagcgc gtccgccggg caattctcaa
    55441 cacaggcagg accgttttcg cgccccgcac aaaggtcgca tttatgcgcc gtggctttta
    55501 cttttcctgc cgcgacgggt gtcaggacga tttgcatcgt accaaaagga caggccacca
    55561 cgcaggattt acagccaata cacttttgct gattgacctg aatgctgtca tcaacgtggc
    55621 tgattgcgcc attagggcag ctacgggcgc agggcgcatc ttcacaatgg tgacaggtca
    55681 ctgcactacg ttgctgttga tgtttgataa ccgtaattcg gggatgaaaa tggtgttggc
    55741 tcaggacatg ttgctcatca ttgtgagcca tgacacaggc gatttcacaa gcatgacaac
    55801 ccagacattg ctgactgttg gccataataa aacgattcat aacgaccttc ttttttggtt
    55861 gtaaaaacct tattctttat atgagtgttg ttattacccg acttacaggg gaatcggcaa
    55921 tgttcatgtg cccagaataa gtaactattt cgctataaac tgtggcagat caaataatcc
    55981 catcactgac taaattgtgt ttcagcgaac tggaacatta atgatttttt gtggagaaga
    56041 cgcgtgtgat tgttaaacga cccgtctcgg ccagtctggc ccgggccttt ttttacattg
    56101 tgctgctgtc gattctttcc acgggtatcg ctctgctaac tctggcgagc agtttgcgcg
    56161 acgctgaggc tatcaatatt gccggatcgc tgcgtatgca gagttaccgc ctgggctacg
    56221 acttgcaaag tggcagtcca caactcaatg cacatcgcca gttgtttcag caggcactgc
    56281 attcaccggt attaaccaat ctcaacgtct ggtatgtgcc agaagcagta aaaactcgct
    56341 atgcgcatct gaatgccaac tggctggaga tgaataatcg gctcagcaag ggcgatttgc
    56401 cgtggtatca ggccaatatt aataattatg ttaatcagat agacctgttc gtgctggctt
    56461 tacagcacta cgctgaacgc aaaatgctgc tggtggtggc gatttccctg gctggcggta
    56521 tcggtatttt cacgctggtc ttttttactt tgcgccgcat acgccatcag gtggttgccc
    56581 cgctgaatca gctggttacc gccagtcagc gtattgaaca cggacagttc gactcgccgc
    56641 cgctggatac cagtctgccg aatgagcttg gtctgcttgc aaaaaccttt aaccagatgt
    56701 cgagcgagct gcataaattg tacctttcgc tggaggcgtc agtagaagaa aagacccgcg
    56761 atctccacga ggccaagcgt cgtctggagg tgttgtatca atgttcccag gcgctgaaca
    56821 ccagccagat tgatgtgcat tgtttccgcc atattttgca gattgttcgc gacaatgaag
    56881 cggctgaata tctggagtta aatgtcggtg aaaactggcg gattagcgaa gggcaaccaa
    56941 acccggaatt gccgatgcag attttaccgg tgacaatgca agagacggtt tacggcgaac
    57001 tgcactggca aaatagtcac gtttcatcat cagaaccgct gcttaacagc gtttcgtcga
    57061 tgctgggacg cggtttgtac tttaatcagg cgcagaagca ttttcagcag ttattgttga
    57121 tggaagaacg tgcaaccatc gcccgcgaat tgcacgactc gctggctcag gtactttctt
    57181 acttacgtat ccagttgacg ttactgaagc gttcgatacc ggaagataac gccaccgcac
    57241 aaagtatcat ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag ttacgcgagc
    57301 tgttgactac tttccgcctg acgctgcagc aggcggatct cccctccgcg ttgagggaaa
    57361 tgctggatac gttacaaaat caaaccagcg ccaaactgac cctcgactgc cgtctgccaa
    57421 ccctggcgct ggatgcgcaa atgcaggtgc atttgttgca aattattcgc gaagcggtgc
    57481 tgaatgcgat gaagcacgcc aacgccagcg aaatcgccgt cagttgcgtc accgcgccgg
    57541 acggcaatca cacggtttat atccgtgata acgggattgg catcggtgaa ccgaaagagc
    57601 ccgaaggtca ttatggtctg aatatcatgc gcgaacgcgc ggaacggctg ggtgggacgc
    57661 tgactttttc acaaccttcc ggcggcggca cgttagtgag tattagcttt cgctccgcgg
    57721 agggtgagga aagtcagctg atgtaatgcc tcctactgac caaagaatac ttgcacttaa
    57781 ggttcagtat aaaagggcat gataatttac attaactcct ttttttctcc acgattggct
    57841 cgtaccttgc cgctacagtg aagcaagtca agcctacaac gatacgcaga aacacgaggt
    57901 cctcttttaa tggcgaattt ctttattgat cgccccattt ttgcctgggt gctggcaatc
    57961 ctgttgtgtc tgacaggtac cctggcgatt ttctcattgc ccgttgaaca ataccccgat
    58021 ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg gcgcatcggc ccagacgctg
    58081 gaaaacaccg tgacccaggt tatcgagcaa aatatgaccg gcctcgataa tctcatgtat
    58141 atgtcatctc agagcagtgg caccggtcag gcatctgtca ctttaagttt taaagcaggc
    58201 accgatccgg acgaagccgt gcagcaagta caaaaccagc tgcaatcagc catgcgaaag
    58261 ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta aaaccggcga taccaatatt
    58321 ctgaccattg ctttcgtctc taccgatggt tcgatggata aacaggatat tgccgattat
    58381 gttgccagta atattcagga cccgttaagc cgcgtgaatg gcgtcgggga tatcgatgcc
    58441 tatggttcgc aatattccat gcgtatctgg ctggacccgg cgaaactcaa cagtttccag
    58501 atgacggcta aagatgtcac tgatgccatt gagtcacaga acgcgcagat tgcggttggg
    58561 caacttggtg gtacaccttc cgtcgataag caggcgctca acgccaccat taacgcccag
    58621 tcactgctgc aaacaccaga acagttccgc gatatcacct tgcgggtgaa tcaagacggc
    58681 tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg gggcggagaa atacgattat
    58741 cttagccgct tcaatggtaa gccagcctcc gggctggggg taaaactggc ctccggcgca
    58801 aacgaaatgg cgacagcgga gctggtactc aatcgtctcg acgagctggc gcagtacttc
    58861 ccgcacggac tggaatacaa ggtggcgtat gaaaccacct cgtttgttaa agcctccatt
    58921 gaagacgtgg tgaaaacgct gctggaagct atcgctctgg ttttcctcgt tatgtatctg
    58981 ttcctgcaaa acttccgcgc cacgctgata cccactatcg ccgtgccggt ggtgttgatg
    59041 ggaaccttct ccgtacttta cgcctttggt tacagcgtca acaccttaac catgttcgcg
    59101 atggtgctgg cgatcggtct gctggtggat gacgccatcg tggtggtgga aaacgtcgaa
    59161 cgtattatga gtgaggaagg actcactcct cgcgaagcca cacgtaaatc gatggggcag
    59221 atccaggggg cactggtcgg gattgcgatg gttctttcgg cggtatttgt accaatggcc
    59281 ttcttcggcg gcaccaccgg tgccatctat cgccagttct ctattaccat tgttgcggcg
    59341 atggtgctgt cagtactggt agcgatgatc ctcactccgg ctctgtgtgc cacactactt
    59401 aagccactga aaaaaggtga gcatcacggg caaaaaggct tttttgcctg gtttaaccag
    59461 atgtttaacc gcaacgccga acgctacgaa aaaggggtgg cgaaaattct ccaccgtagc
    59521 ctgcgctgga ttgtgattta tgtcctgctg cttggcggca tggtgttcct gttcctgcgt
    59581 ttgccgacgt cgttcttacc gctggaagac cgtggcatgt ttactacctc ggtacaattg
    59641 cccagcggtt caacccaaca acagaccctg aaagtcgttg agcaaatcga gaaatattac
    59701 ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca ctgttggttc tggccccggg
    59761 ggtaacgggc aaaacgtggc gcgaatgttt atccgcctga aagactggag cgaacgcgac
    59821 agtaagaccg gcacctcgtt tgccattatc gagcgtgcaa caaaggcgtt taacaagatt
    59881 aaagaagctc gcgttatcgc cagcagcccg ccagcaatta gcggtcttgg tagttctgcg
    59941 ggttttgata tggagttgca ggaccacgct ggagcaggtc acgatgcgct gatggcagca
    60001 cgtaaccagt tgctggcgct ggcggcggaa aacccggagc taacccgtgt gcgccataac
    60061 ggcctcgacg acagtccgca gttgcagatt gatatcgacc agcgtaaagc tcaggcgctg
    60121 ggcgttgata tcgacgatat taacgacaca ctgcaaaccg cctggggttc gagctatgtt
    60181 aatgacttta tggatcgcgg tcgcgtgaag aaagtctatg tgcaggcagc tgcgccgtat
    60241 cgcatgctgc cagatgacat taatctctgg tatgtccgaa ataaagatgg cggcatggtg
    60301 cccttctctg ctttcgcgac ctcacgctgg gagacaggct cgccgcgtct ggaacgctat
    60361 aacggttatt ctgcggttga gattgttggg gaagccgcac cgggggtcag taccggtacg
    60421 gcgatggata ttatggaatc gttagtgaag cagttgccaa acggctttgg tctggagtgg
    60481 acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg ctccggcgct gtacgccatt
    60541 tccttgctgg tagtattcct gtgtctggcg gcattgtatg aaagctggtc ggtaccgttc
    60601 tcggtaatgc tggtcgtgcc gctgggggtt atcggcgcgc tgctggcaac ctggatgcgc
    60661 gggctggaaa acgacgttta cttccaggtg ggcctgttaa cggtcattgg tttatcggcg
    60721 aaaaacgcca tcctgatcgt cgagtttgct aacgagatga accaaaaagg ccacgacctg
    60781 tttgaagcga cgctccacgc ctgccgtcag cgtttacgcc cgattctgat gacctcgctg
    60841 gcatttatct tcggcgtatt gccaatggca accagcacgg gtgccggttc cggtggtcag
    60901 catgcggtgg gtactggcgt aatgggcggg atgatttcag ctactattct ggctatttac
    60961 ttcgtgccgc tgttctttgt gctggtgcgc cgccgcttcc cgctgaagcc gcgcccggaa
    61021 taagcaataa aaaaggcgac atgccaatgt gtcgcctttt tcaactttcc gattaagaac
    61081 ctgctcagcg ggttcttgct gtttatactt tgtctcggga attacttacg aagcataact
    61141 tcgataaagt ctttccagtt ccccagttca cgttcaatca taacaacctc tcttataatt
    61201 atgggtattc tacggaaaca atataccgtg gtgaagctaa tttactcgat tgctgcgatg
    61261 actacctccg ggggacaaac cttatgtaaa tactatggtc ctgcggtgat aatttgtatg
    61321 tgatacacag caacatttcg agatattcat acggcatcta atactgattt aattctggtt
    61381 aaaata
//
LOCUS       DAPGWD010000011        58102 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPGWD010000011 DAPGWD010000000
VERSION     DAPGWD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.011"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            176..847
                     /locus_tag="RHX88_000635"
     CDS             176..847
                     /locus_tag="RHX88_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDV2229367.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            857..2053
                     /locus_tag="RHX88_000636"
     CDS             857..2053
                     /locus_tag="RHX88_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDV2229368.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2031..2612
                     /locus_tag="RHX88_000637"
     CDS             2031..2612
                     /locus_tag="RHX88_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV2229369.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2614..3387
                     /locus_tag="RHX88_000638"
     CDS             2614..3387
                     /locus_tag="RHX88_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDV2229370.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3572..3847
                     /gene="frmR"
                     /locus_tag="RHX88_000639"
     CDS             3572..3847
                     /gene="frmR"
                     /locus_tag="RHX88_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDV2229371.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3882..4991
                     /gene="frmA"
                     /locus_tag="RHX88_000640"
     CDS             3882..4991
                     /gene="frmA"
                     /locus_tag="RHX88_000640"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDV2229372.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5084..5917
                     /gene="fghA"
                     /locus_tag="RHX88_000641"
     CDS             5084..5917
                     /gene="fghA"
                     /locus_tag="RHX88_000641"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV2229373.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6143..6682)
                     /locus_tag="RHX88_000642"
     CDS             complement(6143..6682)
                     /locus_tag="RHX88_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDV2229374.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6784..7995)
                     /gene="mhpT"
                     /locus_tag="RHX88_000643"
     CDS             complement(6784..7995)
                     /gene="mhpT"
                     /locus_tag="RHX88_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDV2229375.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8270..9283)
                     /gene="dmpG"
                     /locus_tag="RHX88_000644"
     CDS             complement(8270..9283)
                     /gene="dmpG"
                     /locus_tag="RHX88_000644"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDV2229376.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9280..10230)
                     /gene="mhpF"
                     /locus_tag="RHX88_000645"
     CDS             complement(9280..10230)
                     /gene="mhpF"
                     /locus_tag="RHX88_000645"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDV2229377.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10227..11036)
                     /gene="mhpD"
                     /locus_tag="RHX88_000646"
     CDS             complement(10227..11036)
                     /gene="mhpD"
                     /locus_tag="RHX88_000646"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDV2229378.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11046..11912)
                     /gene="mhpC"
                     /locus_tag="RHX88_000647"
     CDS             complement(11046..11912)
                     /gene="mhpC"
                     /locus_tag="RHX88_000647"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDV2229379.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11930..12874)
                     /gene="mhpB"
                     /locus_tag="RHX88_000648"
     CDS             complement(11930..12874)
                     /gene="mhpB"
                     /locus_tag="RHX88_000648"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDV2229380.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12876..14540)
                     /locus_tag="RHX88_000649"
     CDS             complement(12876..14540)
                     /locus_tag="RHX88_000649"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDV2229381.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGGSFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14617..15564
                     /gene="mhpR"
                     /locus_tag="RHX88_000650"
     CDS             14617..15564
                     /gene="mhpR"
                     /locus_tag="RHX88_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDV2229382.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLAARPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15641..16723
                     /gene="lacI"
                     /locus_tag="RHX88_000651"
     CDS             15641..16723
                     /gene="lacI"
                     /locus_tag="RHX88_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDV2229383.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16846..19920
                     /gene="lacZ"
                     /locus_tag="RHX88_000652"
     CDS             16846..19920
                     /gene="lacZ"
                     /locus_tag="RHX88_000652"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDV2229384.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19972..21225
                     /gene="lacY"
                     /locus_tag="RHX88_000653"
     CDS             19972..21225
                     /gene="lacY"
                     /locus_tag="RHX88_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDV2229385.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTVNNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSIRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(21304..22485)
                     /gene="cynX"
                     /locus_tag="RHX88_000654"
     CDS             complement(21304..22485)
                     /gene="cynX"
                     /locus_tag="RHX88_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDV2229386.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPMQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(22491..22961)
                     /gene="cynS"
                     /locus_tag="RHX88_000655"
     CDS             complement(22491..22961)
                     /gene="cynS"
                     /locus_tag="RHX88_000655"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDV2229387.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(22992..23651)
                     /gene="cynT"
                     /locus_tag="RHX88_000656"
     CDS             complement(22992..23651)
                     /gene="cynT"
                     /locus_tag="RHX88_000656"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDV2229388.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23761..24660
                     /gene="cynR"
                     /locus_tag="RHX88_000657"
     CDS             23761..24660
                     /gene="cynR"
                     /locus_tag="RHX88_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDV2229389.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24700..25983)
                     /gene="codA"
                     /locus_tag="RHX88_000658"
     CDS             complement(24700..25983)
                     /gene="codA"
                     /locus_tag="RHX88_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDV2229390.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(25973..27232)
                     /gene="codB"
                     /locus_tag="RHX88_000659"
     CDS             complement(25973..27232)
                     /gene="codB"
                     /locus_tag="RHX88_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDV2229391.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27469..29355)
                     /gene="prpE"
                     /locus_tag="RHX88_000660"
     CDS             complement(27469..29355)
                     /gene="prpE"
                     /locus_tag="RHX88_000660"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDV2229392.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29395..30846)
                     /gene="prpD"
                     /locus_tag="RHX88_000661"
     CDS             complement(29395..30846)
                     /gene="prpD"
                     /locus_tag="RHX88_000661"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDV2229393.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(30880..32049)
                     /gene="prpC"
                     /locus_tag="RHX88_000662"
     CDS             complement(30880..32049)
                     /gene="prpC"
                     /locus_tag="RHX88_000662"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDV2229394.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            complement(32094..32984)
                     /gene="prpB"
                     /locus_tag="RHX88_000663"
     CDS             complement(32094..32984)
                     /gene="prpB"
                     /locus_tag="RHX88_000663"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDV2229395.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            33223..34809
                     /gene="prpR"
                     /locus_tag="RHX88_000664"
     CDS             33223..34809
                     /gene="prpR"
                     /locus_tag="RHX88_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDV2229396.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(34907..35182)
                     /locus_tag="RHX88_000665"
     CDS             complement(34907..35182)
                     /locus_tag="RHX88_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2229397.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35329..36000
                     /locus_tag="RHX88_000666"
     CDS             35329..36000
                     /locus_tag="RHX88_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDV2229398.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36017..36262)
                     /locus_tag="RHX88_000667"
     CDS             complement(36017..36262)
                     /locus_tag="RHX88_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229399.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(36675..37490)
                     /locus_tag="RHX88_000668"
     CDS             complement(36675..37490)
                     /locus_tag="RHX88_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229400.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWLDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(37733..38782)
                     /gene="yahK"
                     /locus_tag="RHX88_000669"
     CDS             complement(37733..38782)
                     /gene="yahK"
                     /locus_tag="RHX88_000669"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDV2229401.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(38869..39825)
                     /locus_tag="RHX88_000670"
     CDS             complement(38869..39825)
                     /locus_tag="RHX88_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2229402.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(39822..40793)
                     /locus_tag="RHX88_000671"
     CDS             complement(39822..40793)
                     /locus_tag="RHX88_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2229403.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(40786..42270)
                     /locus_tag="RHX88_000672"
     CDS             complement(40786..42270)
                     /locus_tag="RHX88_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV2229404.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGWKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVEGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42319..43302)
                     /locus_tag="RHX88_000673"
     CDS             complement(42319..43302)
                     /locus_tag="RHX88_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2229405.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(43556..43966)
                     /locus_tag="RHX88_000674"
     CDS             complement(43556..43966)
                     /locus_tag="RHX88_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV2229406.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(44265..45302)
                     /locus_tag="RHX88_000675"
     CDS             complement(44265..45302)
                     /locus_tag="RHX88_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="metallo-dependent hydrolase superfamily; deaminase
                     with metallo-dependent hydrolase domain; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HDV2229407.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALARISPRTATFH
                     KGQLVWGSVAG"
     gene            complement(45312..46262)
                     /locus_tag="RHX88_000676"
     CDS             complement(45312..46262)
                     /locus_tag="RHX88_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDV2229408.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(46405..47823)
                     /locus_tag="RHX88_000677"
     CDS             complement(46405..47823)
                     /locus_tag="RHX88_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV2229409.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIGFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(47823..49370)
                     /gene="fdrA"
                     /locus_tag="RHX88_000678"
     CDS             complement(47823..49370)
                     /gene="fdrA"
                     /locus_tag="RHX88_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV2229410.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQFAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(49360..50223)
                     /locus_tag="RHX88_000679"
     CDS             complement(49360..50223)
                     /locus_tag="RHX88_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV2229411.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(50263..50868)
                     /locus_tag="RHX88_000680"
     CDS             complement(50263..50868)
                     /locus_tag="RHX88_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDV2229412.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            51126..51623
                     /locus_tag="RHX88_000681"
     CDS             51126..51623
                     /locus_tag="RHX88_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV2229413.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            51715..52647
                     /locus_tag="RHX88_000682"
                     /pseudo
     CDS             51715..52647
                     /locus_tag="RHX88_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(52689..53777)
                     /locus_tag="RHX88_000683"
     CDS             complement(52689..53777)
                     /locus_tag="RHX88_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV2229414.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(53919..57902)
                     /gene="ehaA"
                     /locus_tag="RHX88_000684"
     CDS             complement(53919..57902)
                     /gene="ehaA"
                     /locus_tag="RHX88_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HDV2229415.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKAIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGYYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYCAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT        13811 a        15784 c        14489 g        14018 t
ORIGIN      
        1 acatatgaat tgcatagaaa cattacccag tcgaacctcc gggtctgaaa gcccttgctt
       61 ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc agagattacg
      121 tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc attaagtgga
      181 taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta tccttgctat
      241 tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc gtcttgcgca
      301 aaaaggaatt tatatcgcag ccgtggtgat gactaccggc aactctggca cagatggaat
      361 aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgcc accaaactat
      421 tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga tttccgccct
      481 ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg tatataccat
      541 gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa tggttgcctg
      601 ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt catttatgcc
      661 tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg cattaaaaaa
      721 acataaaagc caggaacgac gcgattatat gcgtcatgat cgcctgcgtg cagttgcaca
      781 atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc ataaaatgat
      841 tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag caatgttgct
      901 atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta ttgacgccat
      961 tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc tactgcgtaa
     1021 cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata ccgaagcggt
     1081 catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgc aaaaaaatac
     1141 cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc aggttttttt
     1201 aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata tgctggcaga
     1261 aattgagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga aaggcgcggg
     1321 gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga agcgcacgct
     1381 acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa tgttccgcac
     1441 tgatgtattg cgtaagttcg gtttctcaga tcgtactaaa gtcgaagacc ttgatctcac
     1501 ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca tcgtataccc
     1561 acaggaatgc aacagcccgc gtgaggagtg gcgtcgctgg cggcgttgga ttgtgggata
     1621 cgcggtctgt atgcgcctgc ataaaagact tttatttagc cgcttcggta tcttcagtat
     1681 atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta cctggtttaa
     1741 tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc ttatctggat
     1801 cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct ggttgttggt
     1861 gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct ggattattta
     1921 tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca cccgctattc
     1981 cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa ctgaaaaact
     2041 atctgaagct taatgttaaa gagaaaaata ttcagattgc agaccaggta attattgatg
     2101 aaactgcagg tgaagttgtt atcggcgcga atacccgtat ttgtcatggt gccgttattc
     2161 agggtccggt agtgattggc gcaaactgcc tgataggtaa ttatgcgttt attcgtcctg
     2221 gcacaataat cagcaatggt gtaaaaattg gttttgccac cgaaattaaa aatgcggtta
     2281 ttgaagcgga agcaacgatt ggtccgcaat gttttattgc cgactcggta gttgcaaacc
     2341 aggcatattt gggcgcacaa gtacgtacca gtaatcatcg tctggatgaa cagcctgtgt
     2401 ctgttcgaac tccagaggga attatcgcta ccggatgcga taaattaggt tgttatatcg
     2461 ggcagcgttc acgccttggc gtacaagtta taattttgcc tgggcgaatt atttccccga
     2521 acacgcaact tggcccgcgc gtgattgtag aacgtaattt acctgccgga acttattcac
     2581 tccgacaaga acttatccgt acaggagatt aaaatgataa aacggacgtt attagcggcg
     2641 gccattttta gcgcattgcc cgcttatgcc gggttaactt ccattaccgc gggctacgat
     2701 tttaccgatt attctggcga tcatggcaac cgtaatttag cgtatgctga actggtggcg
     2761 aaagttgaaa acgcaacgct gctttttaat ctttcccagg ggcgtcgtga ttatgaaact
     2821 gaacatttca atgccactcg cggtcagggt gcggtctggt ataaatggaa taactggctg
     2881 acaacccgaa cgggtattgc ctttgcggat aatacgccgg tctttgcccg ccaggatttt
     2941 cgtcaggata ttaacctggc cctgttgcca aaaacgcttt tcacgaccgg ttatcgctac
     3001 actaaatatt acgatgatgt cgaagtcgat gcctggcaag gcggcgtatc actctatact
     3061 ggcccggtga tcaccagcta ccgctatacc cattatgact ccagcgatgc aggtggtagt
     3121 tatagcaata tgatttccgt gcgtctgaat gacccgcgcg gcactggtta tacgcaacta
     3181 tggctaagcc gcggaacagg tgcttacacc tatgactgga cgccagaaac acgctacggc
     3241 agcatgaaga gcgtcagtct gcaacgtatt caaccgctga ctgagcaact taatctcggg
     3301 ctgacggcag gtaaagtgtg gtacgacacc ccaaccgatg attataacgg tctgcaactt
     3361 gcagcccatc tgacctggaa attctgattc cttctgccgc ccgctatccg gggcggcctt
     3421 ccctgccgat tagccccccc tttcctcttt gttttccgac cacattcacc ggataaattt
     3481 tattctccag tgttatatac tatagggggg tatgcattga catatagcat acccccctat
     3541 agtatattgc gtgcagatga tgaggtgcga aatgcccagt actccggaag agaagaaaaa
     3601 ggtccttact cgagttcgtc gtattcgagg gcagattgat gctctggaac ggtcgctgga
     3661 gggtgatgcc gaatgccgtg ccatactcca acagatcgct gccgttcggg gcgcggctaa
     3721 tgggctgatg gcagaagtgc ttgaaagcca tatccgggaa acgtttgacc gaaatgactg
     3781 ctacagccgc gaagtcagcc aatccgttga cgacactatt gaactggttc gagcctatct
     3841 taaatagctg aatctattac catattgagg aagagcgaga gatgaaatca cgtgctgccg
     3901 ttgcatttgc tcccggtaaa ccgctggaaa tcgttgaaat tgacgttgca ccaccgaaaa
     3961 aaggtgaagt gctgattaaa gtcacccata ccggcgtttg ccataccgac gcatttaccc
     4021 tctccgggga tgacccggaa ggtgtattcc cggtggttct cggtcacgaa ggggccggcg
     4081 ttgtggttga agtcggtgaa ggcgtaacca gcgtcaaacc tggcgaccat gtgatcccgc
     4141 tttacaccgc ggagtgcggc gagtgtgagt tctgtcgttc tggcaaaact aacctctgtg
     4201 ttgcggttcg cgaaacccag ggtaaaggct tgatgccaga cggcaccacc cgtttttctt
     4261 acaacgggca gccgctttat cactacatgg gatgctcaac attcagtgaa tacaccgtgg
     4321 tcgcggaagt gtctctggcc aaaattaatc cagaagcaaa ccatgaacac gtctgcctgc
     4381 tgggctgtgg cgtgaccacc ggtattggcg cggtgcacaa cacagctaaa gtccagccag
     4441 gtgattctgt tgccgtgttt ggtcttggcg cgattggtct ggcagtggtt cagggcgcgc
     4501 gtcaggcgaa agcgggacgg attatcgcta tcgataccaa cccgaagaaa ttcgatctgg
     4561 cgcgtcgctt cggtgctacc gactgcatta acccgaatga ctacgacaaa ccgattaaag
     4621 atgtcctgct ggatatcaac aaatggggta tcgaccatac ctttgaatgc atcggtaacg
     4681 tcaacgtgat gcgtgcggcg ctggaaagtg cgcaccgcgg ctggggtcag tcggtgatca
     4741 tcggggtggc aggttctggt caggaaatct ccacccgtcc attccagttg gtcactggtc
     4801 gcgtatggaa aggttccgcg tttggcggcg tgaaaggtcg ttcccagtta ccgggtatgg
     4861 ttgaagatgc gatgaaaggt gatatcgatc tggaaccgtt tgtcacgcat accatgagcc
     4921 ttgatgaaat taatgacgcc ttcgacctga tgcatgaagg caaatccatt cgaaccgtaa
     4981 ttcgttactg atttcccgca ggtatacccc gtccacttca gacggggttc ttaatactct
     5041 ccctgggcag ccgtccgggg gattaaccgt gagataatga ctgatggaac tcattgaaaa
     5101 acatgccagc tttggcggct ggcaaaatgt gtatcggcat tattcccaat cactgaaatg
     5161 tgaaatgaat gtcggcgtct atctcccacc aaaagccgcg aatgaaaaat tgccggtgct
     5221 gtactggctt tcgggcctga cctgcaacga gcagaatttc attactaaat cggggatgca
     5281 gcgttacgcg gctgaacaca acattattgt tgttgcgccg gacaccagtc cgcgaggcag
     5341 tcatgtcgca gatgccgacc gttacgatct cgggcaaggt gccgggtttt acctgaacgc
     5401 aacgcaagcg ccgtggaatg aacattacaa aatgtatgac tatatccgca acgagctgcc
     5461 ggatttagtg atgcaacatt ttccggcaac gaccagaaag tctatctctg gtcattctat
     5521 gggcgggctg ggcgcgctgg tgctggcgtt acgtaaccca gatgaatatg tcagcgtctc
     5581 ggcgttttcg cccattgtct ccccatcgca agtaccgtgg ggacagcaag cctttgccgc
     5641 atatcttggt gaaaataaag atgcctggtt ggattacgat ccggtgagtc ttatttcaca
     5701 aggtcaacgc gttgcggaaa tcatggttga tcaggggttg agtgatgatt tttacgcgga
     5761 acagctgcgg actccaaatc ttgaaaagat ctgccaggag atgaatatca agacgttaat
     5821 ccgttatcac gagggttacg accacagcta ttattttgtc tccagtttta ttggcgagca
     5881 tattgcctac cacgccaata aactgaatat gcgttgataa tagtgcacga ctgccggatg
     5941 cggcgtgaac gccttatccg gcctacactt cgcccgtaaa ccgtaggcct gataagacgc
     6001 gcaaagcgtc gcatcaggca tggcgcacga ctgccggatg cggcgtgaac gccttatccg
     6061 gcctacactt cgcccataaa ccgtaggcct gataagacgc gcatagcgtc gcatcaggca
     6121 tctgcgcacg acttatctat gtttaccaca tcaaatcatc aggcactttg aagtcggcat
     6181 acggatcgtc ttcatcctgc tcttcggcac tgagcgcgct gtgcaacaca atactgctgg
     6241 catcacgctg agcaatttta tcggcaacgc tggcgggaat aatggcgtat tcaccttcgc
     6301 tattgttatc gaccaacaag cgggcaatcg ccagacgacc attaatcaac tgcgcctgtg
     6361 tgagcttatc gacaaaaatc ttcttaatca gattgccgtc agtgaagtta aaaccaatat
     6421 caccattggc aatggtgatt cggttcattt caataagctg cttcacctgg gctttatatt
     6481 ctttcgccaa cgccgcttgt ttttgctgtt cgctaagctg tttatcacgc tcaagctgtg
     6541 cctttttatt ttcttctacc gccgcccgag cttcacgcgc ctgaacgcgc gattttttcg
     6601 ccgttctctc gaccttcgcc gcttttttgc tggtgactaa tcctgctttg agcaactgct
     6661 cttgtaaggt aagttttgcc atctttgttt ccaaacccgc tgaataattt ttagcgagta
     6721 tacctgtaaa cgccgtgggt gtattggggg caagtccctt tcgcccctgc taaataaggc
     6781 gcgtcaggca tcggcacacg gctgcattct tgatctccga ctcatcaaaa taaacaccgc
     6841 caacccagca acaagaatac ccggtgcaga agcggccatt acgccgaccg tgccagtgcc
     6901 taatgccagc attttcccgg ccagtaacgg accgctcata gcccccagac gccctaccgc
     6961 cacggctgtt cccacacctg ttgcgcggat ctgcgaactg taaaacaacg gtgccagggc
     7021 atacaaaacg ctttgcccac ctgtcgcaaa caaccccgcg acaaatcccg ccagcaacat
     7081 accgttaaat gacgacaccg ttccaagcgc cagcagcgaa gctaacatgc cgctataaat
     7141 cagtagcgac atggttactg gacgcagctt atccatcaat gcgcccaaca ttaacgtccc
     7201 gcttgccgcc cccatttgca gggcaaacat cacccctgcc gcctgcgatg gctggaatcc
     7261 ttgctccacc aaaagtagcg gtagccagtt gatcaacata taaaccacca gcagagtgaa
     7321 gaaataacac aaccacagca gcagcgtcgc ggttgccgtt tctggcgcaa ataaggcacg
     7381 cagtggtggc gcagcctgtt tttcgccagc gaaaaccgct gactccggca gccagcgcat
     7441 taatagcggc accagaatca acggcaccac accacctacc caaaacaccg tttgccatgc
     7501 taagtttgcc cccgcgaaac ccagtgtcgc cgccagcgcc gcgccaatgg gaacaccgca
     7561 atacatcagg ctcactgccg tcccacgaaa acgtggaccc gcggcttcag acgtcagggc
     7621 gataagattc ggcaacgccg cccccagccc gacaccggtc atcagccgcg caaagaccag
     7681 tgaggggaaa tcccaggcaa tcgccgttgc cagtgagaac aaaccaaaca gcgcaactga
     7741 gccaatcaaa atgcgcttgc gaccataacg gtccgccagc attccgccaa ccaacgcgcc
     7801 gggtagcaaa ccgagtattc cggcgctgaa tatccagccc atttgcattt tatcgagtgc
     7861 gaaagcctgg gcgatgccac ccgccgcaat gccagccgcc tgaagatcca gcccttccat
     7921 cagagcgacc aaaaaacaaa gcccgatggt cagcatcagg cgggatgaag atgatgaagg
     7981 ggtacgagtc gacataagat gaacctcgtt gcgagtgcag gtgaatcgcg actgatacat
     8041 tgccagccgc gtaatcagaa ctatttaatg actgcctacg ggcgaaatgt aggccggaca
     8101 agacgtttat cgcatccggc agtcgtgtgc agatgcctga tgcgacgcta acgcgtctta
     8161 tcaggcctac aaattctcac gccacccgta ggccggataa ggtgtttacg ccgcatccgg
     8221 cagtcgtgct gggatgggca tacaaaatgc acgttctcgg cagtcatcat tatttgttgt
     8281 tgcgcagatc cagcgccacg tcaacgatca tatcctcctg gccgccaacc atccggcgtt
     8341 tgcccagctc aacgagaata tccaccgcac ttaagccata acgcgccgcc gccgtttcac
     8401 agtgacgcag gaagctcgag taaacaccag cgtatcccag cgccagcgtt tcgcgatcga
     8461 ctcgtaccgg tcgatcctgc aacggacgca ccaggtcgtc ggcggcatcc attaacgcat
     8521 agagatcggt cccatgctgc cagcccagtt tatccgcggc ggcaataaac acttccagcg
     8581 gtgcgttacc tgcgcccgcg cccattcccg cgaggctggc gtcgattcgg tcgcagccct
     8641 cttccaccgc cgcgatagag ttcgccacgc caagactcag gttatggtga gcgtgcatgc
     8701 cagtttgcgt ttctggtttc agctctgctt tcagggcgcg gaaacggtca cggatatcgc
     8761 tcatgttcat cgcaccgcca gaatccacca cataaataca ggtcgcaccg tagccttcca
     8821 tcagctttgc ctgcttggcg agattctccg gcgtggtcat atggctcatc atcagaaaac
     8881 caacggtgtc cattccgagc tcgcgggcat actgaatatg ctgggcggaa acatcagctt
     8941 cggtacagtg cgttgccaca cgaaccaccc gcgcgccagc ctgccaggca tttttcagat
     9001 cgtgaatagt gccgattcct ggcagcaaca acgtcgcgat tttggcgtgc ttcaccacat
     9061 ccgccgctgc ttcaatccat tcaaggtcgc tatgtgcgcc gaaaccatag ttaaagctgg
     9121 aaccttgcaa accgtcgccg tgggccactt caatcgaatc cacgcgggca tcgtccagtg
     9181 ctttggcaat ctggcgaacg ttttccagcg aatactgatg acgaatggcg tgcataccgt
     9241 cacgcaatgt gacgtccgag atataaagtt ttttaccgtt catgccgctt ctcctgcctt
     9301 gcgcgccagt gactgggcca ttttttccgc tgtcgccagc gcactggaag tcataatatc
     9361 gaggttgccc gcataggcag gcagataatg cgctgcgcct tcgacttcca gccagaccgc
     9421 tgttttcagt ccggagaatt gccccacgcc cggtaaattg accggtttat cctgcgggat
     9481 aacttcaaac tgcacgcgct gtttcaggcg ataacccggt acgtaagcct gcaccgcctc
     9541 agccatttca ttgattgagg cttcgatatc atcttgtgaa gcttcgtcgc tcaatacata
     9601 caccgtgtca cgcatcatca acggtggctc tgctgggtta agcacaataa tcgccttccc
     9661 ttttgccgcg ccgcccacca cttcaatggc tcgggaagtg gtttccgtaa attcatcgat
     9721 attggcacgc gtgccaggtc cggcagattt actggcgata gaagcgataa tttcggcgta
     9781 atgaacacgc gccacgcgtg aaactgccgc caccattgga atggtggcct ggccgccgca
     9841 ggtgaccatg ttgacgttca gttgatcgac gttcgcctcg aggttaacca ccggcacgca
     9901 gtaagggccg atggcagcag gcgtcaggtc aattaagcga atatccggtt tcgcttcgcg
     9961 taaagcggca tcgtttttca catgcgcgcc cgcgctggtc gcatcaaata caatgtcgat
    10021 atcagcaaat tcaggcatgt tcatcagccc gatcacccct tcatgggtgg tggcgacgcc
    10081 catacgtctg gcacgcgcca gaccgtcgga ctgaggatca atgccgacca tcaccgccat
    10141 ctccagatgc tgaccgtgac gcaaaatttt aatcatcaga tcggtgccaa tgttgccaga
    10201 accgataatg gcgactttac gcttactcat gacagacttc cttttggggc tgcgcttgaa
    10261 aatgtcgcag caactgaacc tatgccttca atatgggctt caaaacgatc gcccgcattc
    10321 accgccacca tcgggcctaa tgccccggta agaatgatat ctccggcgcg cagcggttca
    10381 cccagactgg ccattttgcg tgccagccag acggcggcat taagcggatg tcccaggcat
    10441 tcgctgccgc gcccgctgga aacctcttcg ttattacgcg tcatcttcat ggcgcagttt
    10501 ttcaggtcta accccgccgg acgttgcgcc ggaccgccga tgacatacac cccgcaggaa
    10561 gcgttatctg ccacggtatc gacaaactga atcgaccagt cgcgaatgcg gctccccacc
    10621 acttccagcg ccggaagtac ccattcaatg gcgttataca gttcgtcgaa ggtgatatca
    10681 gttgcgggta aatcgcggtt caacaccagt gcgatctccg cttcaatgcg gggttgcaga
    10741 acacgggaaa aaggaatgat ttcgttatcg ccataacaca tgtcggcaaa taacgtccca
    10801 aaatccggtt gatcaacgcc cagttgttgt tgcactttcg gatgcgtcag gcccacttta
    10861 cgccctacca cgcgacgccc ctgcacaacg tcatattgca catttatgtg ctgaatggcg
    10921 taggccgctt cagcgttatc gataccaatc agatcgcgca gcggcgcaat cgcttcgccc
    10981 tgctctgcgg cgcggcgtaa atccgccgcc agttgctcaa gagtatgctt cgtcatgacc
    11041 attccttaag ggcgtgcgag gaaattcagc accaattgat tgaaagcgtc ggcatgttcc
    11101 cactgcgccc agtgaccaca gtcgcggaag atatgcagtt cagaaccggc aatgccggac
    11161 agcagacgca gacccgcatc catcggcaca aagcggtcgt tgcgccccca gacaatcagg
    11221 gtttgcgctt tgatttccgc cagacgtggg ccaaaatccg gaaactgttt cggattagct
    11281 tccaggctct taacgaagtt ttccaggtga tcgcggcgcg acagcatatt attcaggcgc
    11341 gcttcaaaca gggcgtcggt caaatcgctg gtatcgaaaa cgaagatatc catcatcagc
    11401 ttcaggtttt cgatagtcgg ctgacgataa agctgattca gtcgcttaat accttcggtt
    11461 ggcatcggcg taaacaaact catgccgccc gtaccgccgc ccatcagcac cagtttgccg
    11521 acgcgctccg gccagttaag ggtgaacgcc acagaactat ggccgcccat cgagttgccc
    11581 agcaggtgga ttttggcgat atccagttga tccaccacgc ttttcaggat tcgtgcatta
    11641 agatccgatc gtgaaccact attaacgatc gaatcgcttt tgccccaacc cggacaatcc
    11701 agcaggatca cccgatagcc cgcctctacc agcggatcga tattgcggct gaagttcgcc
    11761 cagccagtag cacccgggcc ggaaccgtgc agcaggacaa cggtttcgtc gccttgtccg
    11821 cagtcattaa aatggatgcg cagcgtttta cccgcttctt ctacattcag aaaacggctg
    11881 gtggcggctt cggtttgtgg ctgataactc atcatcttct cctgcatatt cagttctctg
    11941 ttctggcgct taacgagcca aatccggcaa tccactccgg gattgggcgg taataacgcc
    12001 cttcgctacg ccagttgcca aatgcagaaa tagcggcaaa agcggcgacc caggttttga
    12061 tttcatgtgt cgactttccg gcaatggcgg aaagctcttc gttactgacg gcatccagtt
    12121 cctgtatgcg tccctgctcc agcaaagtca tgaactggtt atcccaaatc gggttgagcg
    12181 gatgcagagt tctctgatcc tcaacaaact tctcagcggc gctaatcacc cgttgctgac
    12241 gcaattcgcg ctcactggcg ggtaaatctt tcccgctccc caacagacgg tcgcgcatat
    12301 gggcatcggc tttcgccagt tcgggcaccg gcggctgatg ggaaagccca ccggaaccca
    12361 ggaacagcac gcgtttattg agagtgctgg tgaaacgtcc aatggcttca cccaacatgc
    12421 gggtacgctg gaaaccgggc agcggcgtgg cgacaccgtt gatgaacaca ggcagaactg
    12481 gcaccttatc cagcccgccg agcaggaact ccagtggctg ggcgaacccg tggtccacct
    12541 gcatacagta agaaacggca agatcgatcc cgctcttcat gacggcatgc gcacaggcct
    12601 ccgccagctc cacaggcacg ggcagctctc ctgccgcact gccgaaatca ccaattgccg
    12661 tcgctccaac gcctaaacag aacggtggca tcacgtcata gaaaaagccg ttgtagtgat
    12721 caggcgcaaa cagcaccacc agttcagggg agaaagccgc aatacgctcg cgggcgctgg
    12781 caatcacgcc attgacctca tcgagcacct cttgcgccgg gtcgacatac cccaccagcg
    12841 gcgagtggga aagacagtga agataagcgt gcatatcagg ctaccttttc gacagaaacg
    12901 tcggcatcag ggcgggtcag cgtcatcacc gacgccagtt tattcagcgt attgcccaga
    12961 gtttgcggaa tggcggtggc ggcaacaaag cgatccgggc gcatcaccac cagcgaagca
    13021 ttatgttgtg cgaaccagct acgcaggcga ccttgcgtat cgcccacgcg tagcacgccg
    13081 tcgtggttat cctgtgcggt atgaatttgc acttccggca ccacctgaat gaagcgtgtg
    13141 cccaacgcgc gccactgctg gatttgctcg tcgctcatcc cccacagtgg attgcatccc
    13201 cagccaatta ccgcgaagtt cgcgccgatc gcgttatcga gcagcgtcac gtcgccgttt
    13261 tccagcgtga ctttcggctg aataaacatc ttgccgaccg gagagtgctt cgcttcgccc
    13321 tcacgcacca gcgcaccgcc gtaatattgc ggcatcggct tgaagcgcat ttcgaggaag
    13381 tagcgtttta ctggcggcag ataattcaac agccaggaaa cgccgtcacg taacgtaccc
    13441 tgccagcgtt tcggcggagc cagcacgttg cccgccgtca cggacaggtc aatcatcgct
    13501 ttggcgtgat cgcgacgttc ttgttgatag gtatcgagca gcgcatcgcg ggctttcccc
    13561 tggataacca acgccagttt ccaggcgagg ttaaaggcgt cgcgcatacc actgttatag
    13621 ccctgcccct gccacaccgg catgatgtgc gcggcatcgc ccgccagcag tacgcgatca
    13681 atacggaaac gttgcgccag tcgcgcgtta tgggtgtaga cacgctggcg aatcaattca
    13741 acattgtccg gattaggcag cactttgctt aacagcttgc gcatattttg cggctcacgc
    13801 agctgctctt cggtttctcc cggcatcacc ataaattcaa agcgacgtac cgcatgaggc
    13861 agcgcggcag aaacatacgg gcgcaccgga tcgcaacaca aatagatatg cggcgtactt
    13921 aacggatcgt tggcgatatc taccacaatc cactgatttg gcgcagtttt accttcaaac
    13981 ggcacattca gagtgcgacg gacaaaactt cccccaccat cacaggccac cagccactgg
    14041 gctttgacta tttcccgctg cccttctgcc gttttcaggt gcaaggtcac ttcgtcattt
    14101 tgctggctga aggcctccag ctcgcgggca aacaagcagc gcacattcgg aaaacgcgac
    14161 aacccttcca gcatcaccgc atcgacctgt ggctgaataa aggcgttacg gcgcggccag
    14221 ccaaattcat cggtcattgg ctgaatatca gcaaaacagc ggccttttgg ggtgagaaaa
    14281 cgcatcgcgt gccacggtgt agtgtgcggc agaacattct cgaccaggcc gaccgactgc
    14341 atggtgcgca gcgcctcgtc atcaatacca atcgcacgcg ggtagtcgat caacttatcg
    14401 agtttctcca ccaccagcac gtcaatgccc atctgaccga gatagttcgc catcatcagc
    14461 ccaaccggac cggcaccagc gatcgccacc tgaacgctat ggttaacagc aggctggatg
    14521 tcagggtgtt gtattgccat ttcagtacct cacgactcgg acaaaatgcc gttgcgcgca
    14581 cagtacggcg caacttattt tgttaaaaac atgtaaatga ttttttattg tgcgctcagt
    14641 ataggaaggg tgttttcggc tacaatcaaa acatgcccga atgtgcacca ggtgcacaac
    14701 gttgttttaa ctatagaaat gtcaattaat atgcagaaca atgagcagac ggaatacaaa
    14761 accgtgcgcg gcttaacccg cggtctaatg ttattaaata tgttaaataa acttgatggc
    14821 ggtgccagcg tcgggctgct ggcggaactc agcggcctgc atcgcaccac tgtgcggcga
    14881 ctgctggaga cgctgcagga agagggatat gtccgccgta gcccctccga tgacagtttt
    14941 cgtctgacca tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca gtggatttct
    15001 gcactggcgg ccccgctgct gggcgatctg ttgcgcgaag tggtatggcc aacagatgtg
    15061 tccaccctgg atgttgatgc aatggtggta cgcgaaacca cccaccgttt cagccgctta
    15121 tcctttcacc gggcaatggt cgggcgacgt ttaccgctcc tgaaaaccgc ctcgggcctg
    15181 acctggctgg ccttttgccc ggaacaagaa cgcaaggaat taatcgaaat gttagccgcc
    15241 cgccccggtg atgactatca actggcacga gaaccgttaa agctgcaagc cattctggcg
    15301 cgcgcgcgca aagagggtta cggacagaac taccgcggct gggatcagga ggagaagatt
    15361 gcctctatcg ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct gaatctggtg
    15421 tatatggcga gcgcaatgac cattgaacag gcagcggaaa agcatcttcc ggcgctacaa
    15481 cgggtagcaa aacagatcga agaaggggtt gaatcgcagg ctattctggt ggccggacgg
    15541 cgaagcggcg tgcatttacg ttgacaccat cgaatggcgc aaaacctttc gtggtatggc
    15601 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgctata
    15661 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tctcgcgtgg tgaaccaggc
    15721 cagccacgtt tctgcgaaaa cgcgagaaaa agtggaagcg gcgatggcgg agctgaatta
    15781 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
    15841 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
    15901 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
    15961 taaagcagcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
    16021 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
    16081 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
    16141 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
    16201 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
    16261 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
    16321 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
    16381 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
    16441 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
    16501 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
    16561 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
    16621 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    16681 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    16741 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    16801 tgtgtgaaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    16861 gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacacaactt
    16921 aatcgccttg cggcacatcc ccctttcgcc agctggcgta atagcgaaga agcccgcacc
    16981 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
    17041 gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
    17101 gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc
    17161 tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
    17221 ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
    17281 ggcgttaact cggcgtttca tctgtggtgc aacggtcgct gggtcggtta cggtcaggac
    17341 agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc
    17401 gcggtgatgg tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg
    17461 atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat
    17521 ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt
    17581 cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa
    17641 acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtagt
    17701 tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa
    17761 atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa
    17821 gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg
    17881 aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt
    17941 caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt
    18001 aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc
    18061 tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat
    18121 cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg
    18181 cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac
    18241 ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg
    18301 cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg
    18361 cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt
    18421 tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac
    18481 agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc
    18541 ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg
    18601 tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac
    18661 ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag
    18721 cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc
    18781 cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca
    18841 agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa
    18901 ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac
    18961 gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa
    19021 aacctcagtg tgacgctccc ctccgcgtcc catatcatcc cgcaactaac caccagcgaa
    19081 acggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgtca gtcaggcctt
    19141 ctttcacaga tgtggattgg cgatgaaaaa caactgctga cgccgctgcg cgatcagttc
    19201 acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac
    19261 gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag
    19321 tgctcggcag atacacttgc cgacgcggtg ctgattacga ccgctcacgc gtggcagcat
    19381 cagggaaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg
    19441 gcgattaccg ttgatgttga agtagcgagc gatacaccgc atccggcacg gattggcctg
    19501 acctgccagc tggcgcaggt cgcagagcgg gtaaactggc tcggattagg gccgcaagaa
    19561 aactatcccg accgccttac tgcggcctgt tttgaccgct gggatctgcc attgtcagac
    19621 atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg
    19681 aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa
    19741 cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg
    19801 aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtgtcg
    19861 gcggaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa
    19921 taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat
    19981 ttaaaaaaca caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg
    20041 ggagcctact tcccgttttt cccgatttgg ctacatgaca tcaaccatat cagcaaaagt
    20101 gatacgggta ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt
    20161 ggtctgcttt ctgacaaact cgggctgcgc aaatacctgc tgtggattat taccggcatg
    20221 ttagtgatgt ttgcgccgtt ctttattttt atcttcgggc cactgttaca atacaacatt
    20281 ttagtaggat cgattgttgg tggtatttat ctaggctttt gttttaacgc cggtgcgcca
    20341 gcagtagagg catttattga gaaagtcagc cgtcgcagta atttcgaatt tggtcgcgcg
    20401 cggatgtttg gctgtgttgg ctgggcgctg tgtgcctcga ttgtcggcat catgttcacc
    20461 gtcaataatc agtttgtttt ctggctgggt tctggctgtg cactcatcct cgccgtttta
    20521 ctctttttcg ccaaaacgga tgcgccctct tccgccacgg ttgccaatgc ggtaggtgcc
    20581 aaccattcgg catttagcct taagctggcg ctggaactgt tcagacagcc aaaactgtgg
    20641 tttttgtcac tgtatgttat tggcgtttcc tgcacctacg atgtttttga ccaacagttt
    20701 gctaatttct ttacttcgtt ctttgctacc ggtgaacagg gtacgcgggt atttggctac
    20761 gtaacgacaa tgggcgaatt acttaacgcc tcgattatgt tctttgcgcc actgattatt
    20821 aatcgcatcg gtgggaaaaa tgccctgctg ctggctggca ctattatgtc tatacgtatt
    20881 attggctcat cgttcgccac ctcagcgctg gaagtggtta ttctgaaaac gctgcatatg
    20941 tttgaagtac cgttcctgct ggtgggctgc tttaaatata ttaccagcca gtttgaagtg
    21001 cgtttttcag cgacgattta tctggtctgt ttctgcttct ttaagcaact ggcgatgatt
    21061 tttatgtctg tactggcggg caatatgtat gaaagcatcg gtttccaggg cgcttatctg
    21121 gtgctgggtc tggtggcgct gggcttcacc ttaatttccg tgttcacgct tagcggcccc
    21181 ggcccgcttt ccctgctgcg tcgtcaggtg aatgaagtcg cttaagcaat caatgtcgga
    21241 tgcggcactg ggcacatccg acaattattt atagctccat catctggcga aacaagcgtc
    21301 gcatcatgcc tctttgaccc acagctgcgg aaaacgtgct ggtgcaaaac gcagggttat
    21361 gatcatcagc ccaacgacgc acagcgcatg aaacgcccag tccatcaggt aattgccgct
    21421 gatactacgc agcacgccag aaaaccacgg ggcaagcccg gcgatgataa aaccgattcc
    21481 ctgcataaac gccaccagct tgccagcaat agccggttgc gcagagtgat cgagcgccag
    21541 cagcaaacag agcggaaacg cgccgcccag acctaaccca cacaccatcg cccacaatac
    21601 cggcaattgc atcggcagcc agataaagcc gcagaacccc accagttgta acaccagcgc
    21661 cagcattaac agtttgcgcc gatcctgatg gcgagccata gcaggcatca gcaaagctcc
    21721 tgcggcttgc ccaagcgtca tcaatgccag caaggaaccg ctgtactgcg cgctggcacc
    21781 aatctcaata tagaaggcgg gtaaccaggc aatcaggctg gcgtaaccac cgttaatcag
    21841 accaaagtaa acacccagcg tccacgcgcg aggagtgaaa accacgcgaa ccggagtggt
    21901 tgttgtcttg tgggaagagg cgacctcgcg ggtgctttgc caccaccagg caaagagcgc
    21961 aacaacggca ggcagcgccc accaggcgag tgtttgatac caggtttcgc tatgttgaac
    22021 taaccagggc gttatggcgg caccaagtcc accgccgccc atcagagccg cggaccacag
    22081 ccccatcacc agtggcgtgc gctgctgaaa ccgccgttta atcaccgaag gcatcaccgc
    22141 ctgaatgatg ccgatcccca ccccaccaag cagtgcgctg ctaagcagca gcgcactttg
    22201 ggggtaaagc tcacgcatca atgcaccgac ggcaatcagc aacagactga tggcgacact
    22261 gcgacgttcg ctgacatgct gatgaagcca gcttccggcc agcgccagcc cgcccatggt
    22321 gaccaccgga agagcggtca acagggcagc cacgctaaag ctcattccgc tcgcctggcg
    22381 caattgcggt agcagtggcc cgacggaggt gagcagtggt cgcatattaa gaccaatcag
    22441 caccagaacc agcagcatcg tgaacgtctt ttgatgattg cgcatggctg tcagaacggc
    22501 ttggtcggca gatatttacc atctaaggta atgaccgcac gttcgccacc ttccgggtcc
    22561 gccactttct taacgtcgag tttgaagtta atcgcgctga taatgccatc gccaaacttc
    22621 tcatgaacca acgctttcag ggttgtgccg tacacctgca acatttcata gaaacgatac
    22681 atcgttgggt cggttggaat acggtcatca atgcagccac gcagtggaat catctgcaac
    22741 agtagaatgg cgtcttcgtc gagatccagc ttcgccccga ccaggcgggc ggcgtcggca
    22801 ggaagcgctt gctgccccag caaagccgcg gttacgaagg cttctgccag accggtaccg
    22861 tcggcaatct cggcaaatga gagatctttt ttagctttgc tgagcaaaat ggcatcggca
    22921 agatcaagac gaatattgcg gttaatttgt gactgaatca tggtggatct cctgatggtt
    22981 taaaaataag gttacgctgc ggtcggttgg cgtagcggta tggcacaaac gcgaggatta
    23041 gcggccagtg gcacaaactg gcgggttgcg ccgtcaaaag ctgcgatgct gccgctttca
    23101 atgtcgtaga cccagccgtg cagggcgatc cgcccctctt cgagcgccag gcgcaccgat
    23161 ggatgagttt gcaaattagc caactgagca atgacgtttt cacgtaccat cgccgcagct
    23221 tttgacggta aatcggaatg cgggcgcgcc tcattaacga cgcgggctga atcggcataa
    23281 cgcagccagt gggagacggc aggcatatgg tccatgcact gacagctggc aatggcggtc
    23341 atcgcgccac agttggaatg accacaaatc acaatgtcag atacccgaag cgcagcgacg
    23401 gcatactcca ccgaagcaga aacgccaccg ggttccggcc cgtaggaagg gacgatattg
    23461 cccgcgttgc gaataacgaa cagatcgcca ggctcacgtt gcgtcaccag ctcagggacc
    23521 agacggctgt cggagcagga gataaaaagt gtgcgcgggc tttgctgtgt cgccagctgt
    23581 ttaaacaagg cttcccgctt cggaaatgcc tcgcgctgga atttaaggaa tccatcaata
    23641 atctctttca ccgttaacct ctgtctgtct ctggaatgag aggcagacta ctcgccgatt
    23701 gtcataaggt aaaagtctca tttatgatga gttctattgg atttacttat aggttggcga
    23761 atgctctctc gacatatcaa ttattttctt gccgtggctg aacatggcag cttcacccgt
    23821 gccgccagtg cgttgcacgt ctctcagcct gcgctttccc agcagattcg ccagttagag
    23881 gagagtttag gcgtgccgct gtttgaccgt agcgggcgaa cgattcgtct caccgatgca
    23941 ggagaagtct ggcgacagta cgccagccgg gcgttacagg aactgggagc gggtaaacgg
    24001 gcgattcatg atgttgccga tctgacgcga ggatcgctgc gtatcgccgt cacccccacc
    24061 tttacaagct actttatcgg ccctttaatg gcggatttct atgcgcgcta ccccggcatc
    24121 acgctccagc tacaggaaat gtcgcaggag aaaatcgaag atctgctttg tcgcgacgag
    24181 ctggacgttg ggattgcctt cgcgcctgtg cattcgccgg agctggaggc aattccttta
    24241 ctgacagaaa gtttagcgtt ggtggtggcg aaacatcatc cgctggctgc ctgtgaacag
    24301 gtggcgttga gtcgcttgca tgatgaaaaa ctggtcctgc tcagcgcgga atttgccacc
    24361 agagagcaaa ttgaccacta ctgcgagaaa gcggggctac atccacaggt ggtcattgag
    24421 gcgaactcaa ttagcgcggt tctggagctg attcgccgca cttccctttc cacattgtta
    24481 ccagcagcga ttgccacaca acatgacggg cttaaagcga tttctcttgc cccgccacta
    24541 ctggagagaa cggcggtttt gttgcggcgg aaaaatagct ggcagacagc cgccgcgaag
    24601 gcatttttgc acatggcgtt ggaggaatgt gcggatgttg gcgagaatga atcacggtag
    24661 ccactgacac aggtcagtgg ctcttcaaca cagaggaaat taccgtttgt aatcgatggc
    24721 ttctggctgc tccagatata cggtggtttg tgccggttgt gtactggcaa tcaccttgcc
    24781 gccacgtacc gaataacgta ccggaacctg acggcgcagc gcatcaaatc cattttcagc
    24841 cggcaggata atcaggtttg cgctgtttcc ggcggcaatg ccgtaatcct gcaaattcaa
    24901 tgttctggca ctgtggtggg tgattaaatt caggccatcg ttaatctgcc cgtagcccat
    24961 caactggcaa acatgcagcc ccatatgcag cacttgcagc atattcgccg ttcccagcgg
    25021 ataccacgga tcgaagacat catcgtgacc aaagcagacg ttaatgccgg actccagcat
    25081 ctctttaacg cgcgtgatgc cgcgacgttt tggatacgta tcgaaacgtc cttgcagatg
    25141 aatattgacc agcgggttgg cgacaaagtt aataccggac attttcagca agcggaacag
    25201 gcgtgaggta tacgccccgt tataggagtg cattgccgtg gtgtggctgg cggtgactcg
    25261 cgcgcccatg ccttcacggt gcgccagggc agcaacggtt tcgacaaagc gcgactgctc
    25321 gtcatcgatc tcatcacagt gaacgtcgat gagacggtcg tatttttgcg ccagggcgaa
    25381 ggttttatgc agcgactcca cgccgtattc acgggtaaat tcaaaatgcg gaatcgcccc
    25441 cactacatct gcccctaagc gtaacgcctc ttccagcaat gcttcaccgt tgggatacga
    25501 caaaatccct tcctgcggga aggcgacgat ttgtagatca atccacggcg caacttcctg
    25561 cttcacttcc agcattgctt tcagcgcagt tagcgttgca tccgaaacat cgacatgggt
    25621 acgcacatgc tgaatgccgt tggcaatctg ccatttcagc gtttgccatg cgcgttgttt
    25681 cacgtcgtca tgggttaata acgctttgcg ctcggcccag cgttcaatgc cttcaaacag
    25741 cgtgccggac tgattccagt tcggttgtcc ggcggtttgc gtggtgtcca ggtgaatatg
    25801 tggctccaca aacggcggta taactaaacc ttgttcggca tccaggctgt tttcagttat
    25861 gggcatcacg ccggattgcg catcaatggc gctgattttt ccgtcctgca gatgaatctg
    25921 ccacagcccc tctttgcctg gtaaccgggc gttaataatt gtttgtaaag cgttattcga
    25981 cactgttaac ctccacatgc gtcattgctg ctgtcgtttt acgattcaaa atcggattaa
    26041 ggatcagata gctcagcgcg ccacctaata ccgcgttgac cggaacaatt cccggtaacc
    26101 agtggcctgc ggcaatcccc agcgcgaccg ccagaatcgc cacccaattg acactcatca
    26161 tacgcgtggt cgcaaagtgc tcatagcggc gacggttcat cagatagtcg gcgatgatca
    26221 cgccccctac tggaggaata gctgccgaaa ggaaggtcaa ccagccgaca aaattgttat
    26281 acagccataa tgcgcagacc gtaccgataa taccgttgat taccgaaagg gttttgctcg
    26341 acataccggt aatgttggcg aaacctaaac ccgacgcata gagtgcgtta tcgttggtgg
    26401 tccagatatt cagccccagc accacaatcg caggcagcag caggccctga gcaatcatca
    26461 catcagagat atccgccatg cccagtgccg cagcgcccgc tgcaccgaaa ataaacatca
    26521 acgagttgcc gaggaaaaag gccaccatcg ccaccagcac cgccagtttg gcattgcgac
    26581 caaaccggac aaagtcagcg gtgagcgtac ccgcactgat aaatgacccc acaaccagcg
    26641 ccagcgcgac attgaaatct aacggttgtg cgggaacgac cgcttttaat gcgtccaggc
    26701 cgcccatgcc gttaacagcc agccacacgg aataaccgcc caggcaggcg atagccggaa
    26761 ccgcaatcac cgaaagaacc gtcagcgccg aaatgccaaa aaagacggtg acggtcatca
    26821 gtaaaccgga aacggcaatc agcaaattaa tatccagccc ggttgcctta cccaccggaa
    26881 tggcaaacat cgccacaccg acgccaaacc agccaacctg agttccgccc agtagcagtg
    26941 aaggcagcca tgagccttta acaccaaacg agaagcgagc aagaagatga gtggtcaggc
    27001 cggtttttgc gccaatgtaa ccgagaaatg aagtgtaaat accgaggaga agattaccga
    27061 tgagaactgc gaggaagaaa tcatgatagc taagaccggt tccgagagtg ccgccggtcc
    27121 acatactggc ggaaaagaag gttaatccca gcatgacgaa cgtcaatgcc aatacccctt
    27181 tccgcgccga ctgcgggact ggcccctggc taaagttgtt atcttgcgac acgaaattcc
    27241 ccctttttgc tgtttgaaac ccaaaaaatc cgccgcattc tattcatctg aagataaaaa
    27301 gcaatcgttt tcgtggggaa atatattttt tatatgaatg agtgcaaaaa ggtgggggaa
    27361 aaattatcgg tgtaaaaaca gtgggtaggc ataataagac gcggtaagcg tcgcatcagg
    27421 catcggcgca tggtgtcgga tgcgccacgc gacgcatccg acaatacctt actcttccat
    27481 cgcctggcgg atctgatcca acgacgcagg atcatcaatg gtcgtcagat ccccaggatc
    27541 gcgtccttcg caaatcgcct ggatcgtgcg gcgcagcatt tttccggatc gcgtttttgg
    27601 caattgcgag acaaaccaga cgtgcgccgg gcggccaaag ttgccaatct ggctgtccac
    27661 cagcgccata atcgccttct cttgcgagtg cgccacatca cgatcttcca gactgtcgct
    27721 ctctttcgga atgacaaacg ccaccgccac ctgccctttc agcgcatctt tcaccccaac
    27781 caccgccact tcggcaacgc ccggatggct ggagatactc tcttcaatct cgcgcgtccc
    27841 cagccgatgc ccggcaacgt taattacatc gtcagtgcgc ccgagaataa agtgataacc
    27901 gtcagcgtca cggatgcccc agtcaaaggt ggcgtacacc gggcgggaaa acagcgacca
    27961 gtaagtcttc acaaagcggc cgtcgtcgcc ccagatggtc tgaatacacc ccggcggcag
    28021 cggcccttcc accaccagca tccctttctc gttgacgcca cacggttcgc cggtgacttc
    28081 attaagcaac tgcacgttat agccatacat cggcacaccg gggcttccca gacgcgtcgg
    28141 cctgtcgtcc agaccgcgag caatcgccat aatcggccag ccggattcgg tctgccagta
    28201 gttgtcgatg accggcacat ccagcgtatt gctcacccaa ctggcggtcg gctcgtccag
    28261 cggttctcca gccagataga gcacttccag cgacgagaga tcgtgtttgc gaatttcagc
    28321 ggtagggaat tttttcagca cgcgaatggc ggtcggcgct gagaacatcc ggctaacctg
    28381 atatttctcg acgattttcc accacacgcc gcagtccggc caggtcggta atccttcgta
    28441 aacgatagtc gccatccccg ccagcagcgg cgcgtagacg atatacgaat gccccaccac
    28501 ccagccgata tccgaagcac aaaagaacac accgcccgct ttgccgccaa aaatggtgtc
    28561 catcgaggtc gccagcgcca ccgcatatcc gccgacgtca cgctgcacgc ctttaggttt
    28621 gccggtcgtg ccggaggtgt agagaatgca ggaggtttcg ttggattcca gccacgccac
    28681 cggtacccgc gcgccgatgt gttgatggcg caacgacgcg aaatcgacat cccgcccgct
    28741 gacgcgtgcc attttcgcca gcccgcgatc caccagcaaa acatggcgtg gctggtgctg
    28801 cgcctgactt atcgcatcgt cgagcaattt tttataggga atgattttgc caccgcgcgc
    28861 tccggcatca gccgagacaa tcagcaccgg tttagcatca tcaattcgcg ccgccacgct
    28921 gtgcgaggca aatcccccaa acaccaccga atgaatagcg ccaatgcgcg cgcaggccag
    28981 cagagtaata tgcgcttcgg caatcatcgg catatacacc agcacccgat cgccacgctg
    29041 cacgcccagc gagcgcagca ttgacgccac cgcgtttact tcgtcatgaa gctgacgaaa
    29101 ggtaaaggtg cgctcttctt ctgtttccga agagacggca atcagcgcca gcgcctctgg
    29161 ttgtttctcc agccagcggt cgatagcgtt gtggcacaag ttggttcggc cttcacaaaa
    29221 ccaacgggca aacggcggat tgctgtgatc gagcgtttgc gtaaagggcg tctgccagtc
    29281 aatacgccgg gcctgctcgg cccagaactg ctccggttcg ttaatcgaac gctgataaaa
    29341 ttcgctaaaa gacataatgc tctcctgttg aacttacgcc ttaccgctgt ttgcttaaat
    29401 gacgtacagg tcgagatact cattgaccgg catctgttcc aggcgagttc tgtcgagaga
    29461 aacctccaga atgcgctgct gttggcgagt cgggaactgg cgcgcgagat tgattttgaa
    29521 tttatcgacc agtttcggaa taccatcctg acggcggcga gcatgaccaa tggggtactc
    29581 caccaccact tcttcaaatc gtgtgccgtc ggtgaactca agggtaatgg cattggcgat
    29641 ggcgcgtttt tccgggtcgt ggtagtcagc ggtaaatgcc ggatcttcaa agcaattgat
    29701 cttctcgcgc agggcgtcga tacgtttatc ttgcgccacg ttgtcctcgt aatctgccgc
    29761 cgttaagcgc ccgaacagca gcgggatcgc caccatgtac tgaatgcagt gatcgcggtc
    29821 tgccgggtta ttgagcggtc cttttttgtc gatgatgcga atacaggctt cgtgggtgcg
    29881 aatggtcact ttttcgatat ccgccgccgt tttgcctgct gcctgcatct gttcatagag
    29941 cgtcatcgct gcttcaactg ccgtctggga gtggaactcc gccgggaagg agattttgaa
    30001 cagcacattt tccataacgt aggaaccgta cgggcgctgg aagcggaacg attcaccttt
    30061 aaaggagacg tcgtagaagc cccacaccgg cgcagtcagg gctgacgggt aacccatttc
    30121 gcccgttttc gccatcagtg ccagacgtac cgcgcgggaa gtggcatcgc ccgccgccca
    30181 ggatttacgc gtgccggtgt tcggcgcatg gcgataggtg cgcagcgact gaccgtccac
    30241 ccacgccagc gaaacggcgt tgagaatttc ctcgcgggtc aggccgagca tttcggcgac
    30301 cacggcggtg gaagccactt tcactaacag aacgtggtcg aggccgacgc ggttaaagga
    30361 gttttccagc gcgatgcagc cctgaatttc atgggctttg atcattgcgg tcagcacctg
    30421 tttcatggtc aacggcgctt tgccgctggc gaccgcgttg cgcgaaagcc agtccgccgt
    30481 tgccagaatg ccgccgaggt tgtcggaagg atggccccac tccgccgcca gccaggtatc
    30541 gttgaaatcg agccagcgga tcatcgcgcc gatgttaaat gccgcctgga cggggtcgag
    30601 ctggaactga gttccgggga cgcgcacgcc gttgggtacg acggtgccgg gaacaattgg
    30661 ccccagcagt tttttacagg ccgggtattc gagagcttcc agaccgcagc cgagcgtgtc
    30721 gagcaggcag taatgtgcgg tgtcgtaggc cactttagag ctgatttcgt agttcatgac
    30781 gtaatcgacg atatcaacga tttcacgatc aaattccggg cggatgttgt tgatttgagc
    30841 tgacattggg tacgtttcct tattgttatt cgtagaggtt tactggcgct tatccagcgc
    30901 gacaaacggg cggtcttccg gtccaacata attggcggaa ggacggataa ttttgttgtc
    30961 ctgacgttgt tcgataatgt gcgccgccca gccggtgacg cgggcgataa caaacagtgg
    31021 tgtgaacatc tcggtgggaa cgcccatcat gttgtaggaa acagcagaga accagtcgag
    31081 attggggaac atctttttgc tctcccacat caccgtttcc aggcgatcgg cgatgttgta
    31141 catcttcagc gagccgcctt cctgcgagag ctgcttcgcc acacgtttaa tcacctggtg
    31201 gcgcgggtca gcgatggtgt aaaccggatg accaaaacca atgaccactt ctttgttttc
    31261 cacgcgcttg cggatatctg cttcggcttc gtccggcgtt tcgtagcgtt gctggatctc
    31321 cagcgacact tcattcgccc cgccgtgttt tggcccgcgc agtgcgccaa tcgcgccaat
    31381 aatcgcggaa tacatatcag agccggtgcc cgcaatcacc cgactggtaa aggtggaggc
    31441 gttaaactcg tgttcggcgt acagcaccag cgagatatgc atcgcctttt cccagctttg
    31501 cgatggcttt tcgccgtgca gcagatgcag gaagtgaccg ccgatggagt cgtcatcggt
    31561 ttccggctgg atgcgttcgc cgttgtgact gtagtgatac caataaagga gaatcgagct
    31621 aagtgatgcc agcagtttgt cggcaatatc ccgcgcgcca gagacagtgt gcccctcttt
    31681 ttctggcagt gtgcagccga gcgcggaaac gccggtgcgc ataacatcca tcgggtgtga
    31741 cgccgccggt aaggcttcca gcacggtacg cacgttagcc ggtaaaccgc gcagggcttt
    31801 cagtttcgtt ttgtaagcgg cgagttcgtc acgggtcggc agtttgccgt ggatcagcag
    31861 atgcgccact tcttcaaatt cgcaatgttc cgccagatca agaatatcgt agccgcggta
    31921 atgcaggtca ttgccgcttt tacccacggt gcagagcgcc gtattgcccg ccggaacgcc
    31981 agaaagtgcc acagattttt tcggtttaat gacatgggta ctgttttgca ggatcgttgt
    32041 gtcgctcatg ttgtcctcgt catttgtagg gtcgagatat ttttcgttgt gttttatttc
    32101 acctggctac gggcaaacag gtcgtcgagc ttcgcttcgt actggtagta gttgatgctt
    32161 tcgtacagct cgttgcgggt ttgcatggta tcgatgacgc ttttctgcgt gccttcctgg
    32221 cgcaggacgt tgtagacatg ctcagcggcg cggttcatgg cgcggaacgc tgaaagtggg
    32281 tacagcgcca ttgcgacatg ggcgctgcgt aattcgtcgg tggtaaacag cggtgttgcg
    32341 ccaaattcgg taatgttggc gaggatcggc acctgcaccg catcggcaaa ctggcggtac
    32401 atggcgagtt cggtaatcgc ctccgggaac aacatctcgg cacccgcttc aacataggcc
    32461 tgtgcacgct cgatcgccgc atccagcccc tctaccgcca gcgcatcggt gcgcgccatg
    32521 atcacaaaat caggatcggt tttcgcatcc accgccgcgc ggatccgatc caccatctct
    32581 tctttcgaga cgatcgcttt attcggacga tgaccgcagc gtttcgcacc aacctgatct
    32641 tcaatatgca atcctgccgc accggcttta atcatcgatt tcacggtgcg cgccacgtta
    32701 aaggccgaag aaccaaaacc gatatccgca tccaccagca gcggcagcga acaaacgtcg
    32761 gtgatacggc gaatatcggt cagcacgtca tcaagagtag aaataccgag atcgggcagc
    32821 cccagcgaac ctgccgccac gccaccgcca gaaagataaa ttgcctgata tccggcacgc
    32881 tgcgccaaca gcgcatgatt agcgttgatg gtgccaacaa tctgcaatgg attttcttta
    32941 gtcagtgcag cgcgaaacgc tttacctgga gagtgtagag acatagccca tcctttgtta
    33001 tcaacttgtt atttgcgttg ataaagacaa agcaaggggt gtgccacaat tgccttaata
    33061 aaattaaatg tttaatttca atttgttaga gataaagtca tgaactaatc cgcaaatatg
    33121 cgtttcagtt aacgtttcag gcaatgtttc accgcgtttc attgcaacaa ttatgaaaca
    33181 agactaaacc caatattcgg tttcttaact ttgcggtgcg ctatggcaca tccaccacgg
    33241 cttaatgacg acaaaccggt tatctggacg gtatctgtaa cgcgcctgtt cgagctgttt
    33301 cgcgatatca gcctcgagtt tgatcacctg gcgaacatta cccctatcca gcttggcttt
    33361 gaaaaagcgg tgacctacat ccacaagaaa ctggcaaacg aacgctgtga cgccatcatc
    33421 gcggctggat ctaacggcgc gtacctgaaa agccgcctgt cagtgccagt tattttgatt
    33481 aaaccgagcg gctacgatgt gttacaggca ctggcaaaag ccggaaaact cacctcttct
    33541 atcggcgttg tcacttatca ggaaactatt ccggcactgg tggcgtttca aaaaaccttt
    33601 aatttgcgcc tcgatcaacg tagctacatt accgaagaag acgcacgcgg gcagattaac
    33661 gagctgaaag ctaacggcac tgaagcggtg gtcggcgcgg ggctgattac cgatctggca
    33721 gaagaagccg gaatgaccgg aatttttatc tattccgccg ccaccgtgcg ccaggcgttc
    33781 agcgatgcgc tggatatgac gcgcatgtcg ttacgccata acactcacga tgccacccgc
    33841 aacgccctga gaactcgtta cgtgctgggc gatatgctcg gtcaatcacc acagatggaa
    33901 caagtacggc agactatttt gctgtatgcc cgctccagtg cagcggtgtt gattgagggg
    33961 gaaacgggga cgggcaaaga gctggcggcc caggcgattc atcgggaata ttttgcccgc
    34021 cacgatgcgc gacagggcaa aaagtcgcat ccgtttgttg ccgtcaactg cggggcgata
    34081 gccgaatcgc tgctggaagc ggagctgttt ggctatgagg aaggggcgtt taccggctcg
    34141 cgacgcggag gtcgcgccgg gctgttcgaa attgcccacg gcggtacgct gtttctggat
    34201 gagattggcg aaatgccgct acctttgcag actcgcctgt tacgggtgct ggaagaaaaa
    34261 gaggtcaccc gcgtcggcgg gcatcagcct gttccggtag atgtacgggt cattagcgcc
    34321 actcactgca agctggaaga agatatgcag caaggacgtt ttcgccgtga tctgttttat
    34381 cggctgagta ttttgcgtct gcaattgcca ccactgcgcg agcgggtggc ggatattctt
    34441 ccgctggcgg aaagcttttt gaaagtgtct ctggcggcgc tctccgcccc gttttctgcc
    34501 gcattacgcc agggattaca ggcaagcgaa accgtgctgg tgcactacga ctggccaggt
    34561 aatattcgtg aactgcgcaa tatgatggag cgactggcgc tatttttaag tgtggaaccg
    34621 acgccggatt taacgccgca atttttgcag ctgctactgc cggaactggc gcgcgagtcg
    34681 gcgaaaactc ccgctccacg cttactgaca ccacaacagg cactggagaa atttaatggc
    34741 gataaaacag cagcggcgaa ttatttaggc atcagccgga cgacgttctg gcggcggctg
    34801 aaaagctgaa ctgccgaatg cagcgcgaac gctttatccg gcataaattt caatagatga
    34861 tcaccgataa acttacgcat cgggcatgtt tacatagggt tcagaattac ttcttcttat
    34921 aaatatttgc cgtgccgtgg atcttattgt cagtttgacc ggaggtcagt accaacacat
    34981 cagccccttt ttcatccgct tttttgatca aatcttcttt cgcgtctgca gtcgacattt
    35041 cattgctggt tgaaatatca cctatttttt catactgcga ttcaactttt tcaaattccg
    35101 ctttggtcat caattcagcg gcatagacat tggtaacggc taacgctaac gcaccgacta
    35161 acattttaga gattattttc atattacggc tccagagttg ttatcacact atcacccacc
    35221 cacattattg ggttgattaa taattagttc agtcagggca taaacgccag taaccggaaa
    35281 taagacactt tgtgatctct ctcgcacccg attatttttg gtagaaatat gatgaagtta
    35341 cttcacttat ttatggatga aatcactatg gatcctttgc acgccgttta cctgaccgta
    35401 ggactgttcg tgattacttt ttttaatccg ggagccaatc tctttgtggt agtacaaacc
    35461 agcctggctt ccggtcgacg cgcaggggtg ctgaccgggc tgggcgtggc gctgggcgat
    35521 gcattttatt ccgggttggg tttgtttggt cttgcaacac taattacgca gtgtgaggag
    35581 attttttcgc ttatcagaat cgtcggcggc gcttatctct tatggtttgc gtggtgcagc
    35641 atgcgccgcc agtcaacacc gcaaatgagc acactacaac aaccgattag cgccccctgg
    35701 tatgtctttt ttcgccgcgg attaattacc gatctctcta acccgcaaac cgttttattt
    35761 tttattagta ttttctcagt aacattaaat gccgaaacac caacatgggc acgtttaatg
    35821 gcctgggcgg ggattgtgct cgcatcagtt atctggcgag tctttcttag tcaggcgttt
    35881 tctttgcccg ctgtgcgtcg tgcttatggg cgtatgcaac gcgttgccag tcgggttatt
    35941 ggtgcaatta ttggtgtatt cgcgctacgc ctgatttacg aaggggtgac gcagcggtga
    36001 gcagataatt tactctctac gtaatcaacc tgatttgccg aatcccggcc atcggcgatg
    36061 atagcccccg taatgcgtgg tccttatttc cgataagttt acttgagtct ttagcttccc
    36121 atttcagccc actctcccct gtgtgggctt ttttcattgg tctgacccgt cctgaatagc
    36181 caaacaatgg caatgttcca ctttgcaggg atgataataa ggggatctga ttcagaagag
    36241 ttttgaaaag ttggaccgcc atcgaggcct cgaaccccgt accatacaac aatcctggtt
    36301 gtcgtctgct cttcctgatg agctaatggc ggtttttgat atgagcctta cagcatattt
    36361 tctgtcaggc ttgagtgcga tgcgtaagat acagaaaaga ccccacacaa acatgatgga
    36421 attatgattt ttttcatatg aatgtcaacc cacacattca caaaacatct gcatgagatc
    36481 attttttata caccgcatgg ctgcgaaacc actactggtg cttttttaag cgaagtgtcg
    36541 ccgatcacaa caaaatatcg gcgaaaaaag agatggaatg ccagttggca tacagatata
    36601 cagatgaaat gattacctgc attgcgattc actctctggt ggaataacaa cttgcaggtg
    36661 gaaccctttc tgaactaaag agtgtagcgg attttaacgt gcttatttac aggataatat
    36721 ctgtcaccta attcctgcac tctattcgat atccattcat ttaatcgtgc gtcatctctg
    36781 tatgttttta cgtccagaac agagagcaat aacatcacca gtgattcagg aaaaacaggg
    36841 tggatatttt ttaagtgtaa taataatcca tgcgcaagcg cctgcttaat gggcgcgtct
    36901 gtctccggaa tagacgacat ttcgcgaagc catttttcca cacgcataca ctctttccct
    36961 ttagggaaaa ttctcagcaa aggcatatgg tgttcaagaa gaatacattt gcaatctgtt
    37021 cgcactgaat cgataaactg cactaatttc ttaagcccgg taaagcgtcc agcctgcaca
    37081 tttgctttaa tatcaagaaa cgcatcatcc tggtcatata cccaaactgt ccctttttga
    37141 ttttcaagta tgcgaaaaac aaacttaacc gggtgatcag ggtaatccat ccagtcataa
    37201 aatccaggga aatcattaaa ctgcacattt atggtgagtg cgttatcgtc taaccactca
    37261 cagtctcgta cggcagccat gagatgaatg agtttgtcgt catcgactaa atcatcacga
    37321 caatcataag gataatcata atattgttta atcaaaacct tgaatatatc gacacaggaa
    37381 accaaggagc ttttgatatc ctctggtaac tgattgtttt taaccgtatt aagtatgctt
    37441 tgttgcgtat atggcatcaa attattgtgc ggagctttta atgatatcat cgcgatctct
    37501 cttaaataaa aataaacgtc gcagataata aagctctcat taaaccaggc actatcagaa
    37561 atcgctcata actaacgtga gaagtattgt gtactggagg gagttaattt aacaatttga
    37621 atttacgtta taggttttac tgctactttg atattatttc gctctcctta actaaaaagt
    37681 taagtggaga gcgaagttaa tatgttaaac cacagggtat ttattaattt tttcagtctg
    37741 ttagtgtgcg attatcgata acaaaacgat atttcacatc accacgcagc attcgctcat
    37801 aggcttcatt aatttgatcg gcccgaatca tctctatatc agccacgatg ccatgttcgg
    37861 cgcaaaaatc gagcatctcc tgagtttctg gaatgccgcc aatcatagaa ccggctatcg
    37921 cacggcgttt catgatcagg ttgaaaactt ccggcgattt atgcggtgtc gcaggcgcac
    37981 caaccagcgt catggtgcca tcacgcttca gcaaggtggt aaaatcgtcg agattatgtg
    38041 gcgcagctac tgtattcaaa atgaaatcga aactcttcag atgagccgcc atctcatcgg
    38101 cattgcgtga gttaacaact tcatcggccc ccagggcttt tgccgcttcg cgttttgcct
    38161 cagaagtggt aaatgccacc acatgtgccc ccatcgcgtg ggccagctta atccccatat
    38221 gtcccagacc gccgatgccg accacgccca cttttttacc cggcccggcc tgccagtgac
    38281 gtagcggcga atacgtggtg atccctgcac acaacaaagg agccaccgcc gccagctgct
    38341 cttgcgggtg acgaatacgc agaacatatc gctcatgaac gacgatctgt tgtgagtagc
    38401 cgcccagagt atggcccggt tcgtccggcg tcggcgagtt ataggtgccg gtcatgtgat
    38461 cacagtagtt ttccagcccg tcttcacact cttcgcaatg tttacaactg tcgacaatgc
    38521 agccgacacc gaccagatcg cccggcgcat gtttttctac ctgatcacca acggctacca
    38581 cacgccccac aatttcatga cccggtacgc aggggtaaac cgtccccgcc cactcggaac
    38641 ggacctggtg gagatcggaa tggcagacgc cacagtaagc gatttcgatt ttgacatcat
    38701 tcggtcccgg ttcacgccgt gtgatatcca tcggttcaag tggttgttta gcggaatatg
    38761 caccaacagc tttgatcttc attgtgttta ctcctgatta gctatgtgta tttggcgatg
    38821 cctgatatgt catcgcggca aaacgcgtcc attgaatata gccaatattt aaaccacccg
    38881 atccagcccc aacatgccgc gaacctgaat gaaacagagc agcaacgttc cccataaggc
    38941 catcgtcagg taggggctta taccgagaat attgaagccg ctttcaataa tttgcagaag
    39001 aaacagcgcc agcaccatac caattatccg cccgctgccg ccgtccggat taaccccgcc
    39061 gagtactgcc gcgaggattg acaccagcaa gtacgattcg ccataagagg ccttagccga
    39121 gttgagtttc gacatcatca aaaatgccgc taccgcacaa agcaaggcgg aaatgacata
    39181 aacccaaatc agcacccgac gagtgttaat gccggagtaa agtgttgctc gttcgttaga
    39241 cccaatcagg taaatggctt taccgagcgg cgttttctcc agcaatatcc acagccccag
    39301 tgccactgcg gcaaacaacc acatcggtag cggaataccg aaccattgcg catgattcaa
    39361 ccacagcacc catgagggat aattggcaat ggcgctacca cctgtcacca gaatatttat
    39421 ccctttgagc aacgtcatca tcccaagcgt tgcaagaatt ggcgacactc gaataccagc
    39481 aatcaatacg ccgttacata aaccgattat caccgccgcg ccagcgcccg ccagtaacgt
    39541 agcgacaacc gtagcaatac ccggtggata ctgagtcgcc acccacgcca tcaccagtga
    39601 acaggcgtta gcagtggcta taattgacaa gttaatgcca ccgcaaagca tggtcaccgc
    39661 catagccaac gccagaatgc ccagcacggg catttgcgaa gcgacggact ggaaattact
    39721 tatcgaccag aaaatctccg gcatagtaat gctgaacgcg gcaacagtta gcaccagcag
    39781 gccgatcaag taaaactcaa cgttattacg ccacgatttt ttcatagcag actccggttc
    39841 tgatgctgac tccacgccgt ggcactaatg ctggcaacga tgatgatgcc ggtgatcaat
    39901 gtttgccagt aagacgagac tcccaataaa tttagcccgt tttgcatcac tgccaacaac
    39961 accacgccga gcaaagtacc ggttaacgtg ccgcgcccgc cgagcaaact agtgccgcca
    40021 agcaccaccg cagccagtac tgtcagctca taacccagaa gagaatcggg ggcgacagtc
    40081 atcaccgtcc acgactgcac tacacccgca gcgccagaca tcaatcccat atagccgtag
    40141 acgaaaagtt gcagtttcag cacgctaaag ccgatgcgac tggctgattc gcggttcccg
    40201 ccaagggcat aaattttgcg ccctacactg gtgaaattca tgataaacgc tgtcagcagc
    40261 accaccgtaa tcatggcgat cagcggcaga ccaaggccat agtcatagcc atcagcatcg
    40321 gtgtacttga acaacataac cccctgctca aaccacggcg gaaagtcgta cagccacaca
    40381 cctttactca accataacag caggccatag aaaatattca gcgtcgagat agtgatgatg
    40441 atagaaggca cccgtaggcg attaaccagt aaggcgttaa ttaagccaag taaaatgccg
    40501 atgcagcccg ccagtgcaaa cgcgacagcg aagttgccac caattttttg cagcaacagc
    40561 accatgccgt attgcgcaat gatggtcatt gctggaaacg aaatatcaat tccaccggaa
    40621 atcaacacca caaacaaacc acaggcgaga atagtcaaca tggcatagtt attggcgaga
    40681 tcgtacaaat taccgaatgt cagaaactcg tcgctgcgcc aggcaaggcc aacaaacagc
    40741 accacaatca gtaatgccag ccagaattca tggcgttttt tcagttcagc catttaccac
    40801 ctccgcaatc ctggcctgag aactgctgtc aggcaggaag ctatgggtga tctgaccttt
    40861 ttgcattacc aaaatccggt ggctttgata ccacgcttct tcaatttcat cgcagatcat
    40921 caataccgca ataccatgag ccgccagatc gctgataatg ccgtatattc ctgctttgtt
    40981 ggcgatatca acaccgactg tcgggctatc gagaatcaac agtcgtggcc caatcgccag
    41041 ccatttggcg atcgatactc gctgcgcgtt accaccagaa agcgtattca ccggaagatg
    41101 aggatcagag actttgatgg tcaatgcctt gattagcctg tccaccaggt cacgaacttt
    41161 agtttcactc agaaagccga agcgatcttt taccttgtga aaaacagtgc taatgatgtt
    41221 gtcttcaata gattgcgcca tcaccaaacc gcgcgacatg cgatcttccg aaacataacc
    41281 aataccgtgg cggatagcgt cctggttact atgcagtgta accggctggc cgttgatgag
    41341 gatctcgcca gcatccggtc gggtcatacc aaacagactc agacataatt ccgtgcgccc
    41401 cgcaccaagc aggccaacaa tcgataccac ttcgcccgct tccacccgca agttaatatt
    41461 tagatattcc ccatgacgac ttaaattacg cacctccagc accggggtgc tggattttcc
    41521 ctgccacaac tctcgcacct gatattcaaa ctcctggccg gtcatcagga aaccgagttg
    41581 ttttgtggtc atctctgcag ccggaaaagt cccgaccagc tcaccatctt tcagcactga
    41641 aatacggtcg gaaacttcca tcacttcttc cagacgatga ctgacgaaga ccacgcagat
    41701 cccgcgttca cgcaattgat gcaccacctg taataaccct tccacttcct gatgggtcag
    41761 cgaagcggtt ggttcatcca tcacgatcaa ccgtgcgtcc tgcgccagtg cacggcaaat
    41821 agccaccagc tgacagcggg caattgaaag ctcagaaacg agtgtatcca gtggcaatgt
    41881 gacgttaata ctggtcattg ccgcctgcgc cacctcacgc agacgacggc ggttaacaaa
    41941 cagaccgtgg tgatagtgat tcacggcgat attctcccag acactcaggt taggaaataa
    42001 agagaggtcc tgataaatta cctgaatccc ctgcgccacc gaagcggcgg gtgtcagctt
    42061 tgaccagttt ttccaaccaa gcgtaatttc cgctccctca tccgggcgat aaacaccgga
    42121 cataatttta atcaacgtcg atttaccaca gccgttttgc ccggcaaggc agtgcacttc
    42181 acccgacata aagtcgagag aaacatcgcg cagcgctcgc gtggcatgaa acgttttgtt
    42241 gatatgacga agggaaagga aagtttccat aacatcccct ggcaataaga atcggggctt
    42301 tcgccccgaa gaaatggatt aataaagtga gtcgatattg tctttgttga ccagtaatac
    42361 cttatggaaa cggataattc gcttatccat atctacgtcg gccttaccca gattctgcat
    42421 ttctaatcca ggtttgatct cttcaccctt cagcaacgtg ctggcgacgg ctgcgagtgc
    42481 gtaacctgca gaggccggat cgtaagtaat cccttcggtg atatcaccac ttttaatcag
    42541 tgatgccgcc tgtgaaggga tcatcatgcc atagactgcg actttatttt tcgcccgttt
    42601 ctctttcacc gcacgtcccg cgccaatcgg accgtttgaa ccaaaggaga caaccgcttt
    42661 caagtcagga taggttttca tcaggtccag tgtggtacga cgtgagtcat ccacactctc
    42721 ggcaaccggc atgcggcggg taacttcatg catatccggg taatgctctt tctggtattt
    42781 caccagcaag tcagcccata agttatgctg cggcacggtc aaactaccca cgtaaatcac
    42841 atagccgccc ttgcccccca tgcgtttcgc catatgctca acatattcag cggcaaattt
    42901 ttcgttatca atgatttcaa tatcccagtt agcacttggc tgaccggggg attcgttggt
    42961 cagaaccaca attccggcat ctcgcgcttt tttgaatacc ggttccagca cgttggcatc
    43021 gtttggcacg atagtaattg cattaacctt acgggcgatt aaatcctcaa taattttaac
    43081 ttgttgcgga gcatcagtac ttgaaggccc cacctgtgag gcattaacac caaaggcttt
    43141 acccgcctca accacacctt cgcccatgcg attaaaccac ggcataccat cgactttaga
    43201 aatattcacc acgacttttt ccgctgcctg gagcggcgca gaaattagcg cagcgcctaa
    43261 taacagcgaa gacaccatat tgataacaaa acgtttattc atcatttcag cctcattgca
    43321 gggtagtcag gaagtgttcg cccaaattag cgcgctggag agaataagaa ttttcgaata
    43381 tttttatgca gaataaacag ccctatttca ggcagctatt aaaaatatgg ttataaatat
    43441 ggacaaaacg ggcagcaaca ataagtgtga cattcatcac gtaaaaaata gcaaaataaa
    43501 caaaattagc gatattttat gtgtgtgagt ttatttcgcg tagataaata gttatctaca
    43561 caacgtcaat aagggcatat tcttcaaata taacgtccat ttctggtgaa tagcttcctt
    43621 ctgcctggaa aaaacgacgg tgttcattac gccaatattc aagactgagg tcaccttcac
    43681 cttccttacg ggcaagctcc acggtgacat cagaaaagcg cagtaaatgc aggccaatca
    43741 ctctgataac acatacaggc tcaccacggc tattttctac aatcttatac tcaccaatca
    43801 tcggaaatgc gttgtcttca atacatcccg ccagcgaacc acaggaagct gttttaacac
    43861 cagtcgcaat caacgttgcg aggtgatcgg ccaacgctgc tgaatcgcca aaagtacaaa
    43921 aggaagcatg gggatatttt tgtgagaatt tttcttttat ggtcatggca atatttttgc
    43981 gattacggaa aaacaaatca catcttaatt actgttggta accatcagca agagtatcat
    44041 tggtttttcg atactgaatc ttttttatcc gaagcaggtc acatttatgg cctgataaat
    44101 acctttgtta aaactcactt ttgtgtattt ctctcaaata atcccctgga aattatgccg
    44161 cgagattata aagttcagga tcattcatta gcaaaccatt cccgccccac tccctggggc
    44221 ttaaatctct tacaattcac cgttgcaggc gtttcaaatg cgcatcaacc tgccacactc
    44281 ccccacacca gttgcccttt atggaacgtt gcggttctcg gcgagatacg cgccagcgca
    44341 tcgacctgtt gttcgttgag cgtagccagc gcaaaggcgt ggctgatggt cagcttaccc
    44401 ttcagctgtg gcgttttctc taccgtttca accatataat tgatggctgc cacgcccgac
    44461 ggagtggttt cgtgcaggtg aatatcgacg cctttgtcgt agtccagcgc aatctggaac
    44521 atggtgtcga gggatttttc catcgcgcca tcaacactgg tcgggtccag gccgccgacg
    44581 taatgcgccc ccgcctgcat cgcttcacgc atcaaaggtt cagatttcga cagcagcaaa
    44641 ccgtgctgcg ggaaggcgac gatttcacac tcaaagcccg cctgacgtcg cgccagcacc
    44701 gcctgcaaat tttgcagatt tttcaggccg gaaactggtt cgatattgca gtgactgcgg
    44761 gcaatggtgg tgcctttcga ctgcaataga tcgatcagtt tttccgcccg ctcctgcgta
    44821 tacggttgta actccggcag cattttctgc tcgagtttga tcatgtcctg gatagtggtg
    44881 cctgccggac gattgagcga gcgccacggc ccgccgtaga aggttttgtc gagatgaata
    44941 tgcatgtcgc gagtggtggg tagcatcaac ttaccgccag cgtcatagtg cggcagcgtg
    45001 gcgtccggat gctgcttgtt ctcgcgcagg gcgacaattt tgccgtcctg aatctccacg
    45061 gtctggcgcg ccgtgcgggt ctgcaccgcc acaccgtttt cgtagtcaaa accggtttcc
    45121 agcagcacgt tatcgagata gtaatgcggg tcagtgattt tcatggtgtt gttcgcttcg
    45181 cagtttaggg tgccagctac cgccgcatgg gcgagcggca cagaggtacc aaacagcgcg
    45241 gcggcggtga ccatcttacc gctctggctc agaaattcac ggcggctatt gctttctttc
    45301 atcctacgtc cttacgtttt aataatatgg gtgcccgttt caccgcgcag cgccgcaggc
    45361 aggcattccg gcgtggtgat aatcacttct ttgccgccct gctccaggaa cgtcaggctg
    45421 gcgatgattt ttggcaacat gctgccgggc ggaaaatgcc cttcctgcat atagcgagtc
    45481 atggtggcaa tatccacccg atccagcgcc tgctgctgcg gtttaccaaa gtggatgcag
    45541 actttttcga cgccggtggt gatcaccaga atgtcggcgt gaatttcacg ggccagcagc
    45601 gcggtagaga gatctttgtc aataaccgcg tccacgcttt ggtaatcccc cgcttcagta
    45661 cgcactaccg gaattccacc gccgcccgcg ccaattacga caaaaccttg ttggatcagc
    45721 gccttaatgg cgggtgcttc gacaatacgt ttcggttccg gcgaggcgac cacgcggcga
    45781 tagccccgtc cggcatcttc aacaaaacac cagtcagggt ttgccttttg tagtttgtca
    45841 cgctggcttt cactgaagaa tgcaccaatg ggcttggtgg gatgggcaaa acctggatcg
    45901 tttttatcca cttccacctg agttaccacg gtgacggctt ttttctcgcc gtgacgcgcc
    45961 agccggttat tcagcgcctg ttggatcaga tagccgatgc cgccctgcgt atctgccaca
    46021 cagttcgcca gcggcgttaa gggcagccct tcgcgctcgt gggcaatctc cgcacggcgt
    46081 aaatccagcc cgacctgcgg cccgttaccg tgggtcagca caatgtcgta atcggaagcc
    46141 agcatttcca gcaccgtatc ggccacggct ttcaccgcct ccgcctgatg ctcaatcgac
    46201 tggctggcgt tatctttgat aatgctgttg ccaccaatgg caacgaccac aagctctttc
    46261 atgttgttat gtccgctaca gagggtgatt aggttatgac caacgccaga ctgcctgatg
    46321 cgctacgctt atcaggccta caattctcgg gccatccgta ggccggataa ggcgttcaca
    46381 ccgcatccgg cataaacatg taagtcagac gccgtaacgt tcgcaccagc cgagaatggc
    46441 tttctcgaag caggcgaatg gtggatgcac aatgcccgcg ccaatcatgc cgacgcccgc
    46501 atctttatgg gcgatggcgg tgttgatcac cggcagaatg ccgctgctac ccacgcgggt
    46561 gatgtcgatt gccgacggca cgcccataaa accgagcagc ggaatggtga cgttggggtt
    46621 ttcgccaagg gtgatttcgc gcatctgacg ggagaaaccg atagcctctt ccaccgtgcc
    46681 gcccaccagt gcgacgattg caggcgcggt cgccatggca aacccgccaa taccgtaggt
    46741 ttctgtgatg gcgctgtcgc cgatatccag ccccgaatct tccggcttat aaccagcaaa
    46801 catcgggccg atgacctgct gcgctgggcc ggtaaaccac tgccccggca ggccgctgac
    46861 ccgcaggccg aactcgacgc cgttacgcgc catggtggtg accacggtgc tgtattcgat
    46921 gccgtgcgcc gcatccatcg ccgctttaca catcgccatc cacgtcgggc cggagaagta
    46981 gtcgctgctg gcgacaaact cgaacacttc gcgctgttgc tcgacggaat agcctgcctg
    47041 aatgatcccc ggcgtcagtg cctgaatcag cagcgtcgtc ccggcgttat tgcggttatg
    47101 gcactcatcg cccatatgca gcgcctgcgc cagcattaag cgcagatcga tctcaccgat
    47161 aattttcatc gcgtcgcgca gcattggccc ctgcacatcg cgcatccagt tgaggcggtc
    47221 gatcacgctc tgatcgttgg cccccatacg caaaatcttc gccatctgct cgctcatatt
    47281 ggtataagcg atgttgccgt aggttttgtt tttcacgatg tgcataaaca tcgaggccga
    47341 ggtaacgccc gccatcgatc ccacgcagtc gtgttcgtgg cacggcgaga aggtgatctc
    47401 cccggaggcc gccagttcag tcgcctcatc gagatctttc gccagccctt cgaacaccaa
    47461 cgccccggtg accgcgcctt tcatcgcgcc gcacattttt tcccaggtga tcggcggccc
    47521 ggcgtgaaga atggttttcg gcgtcatgcc cggcaccacg ttaatcgcct gatcaaaacc
    47581 aatcagcacc ggatgcgact ggataatgcg ctccagcgcc tgctggttag cggcggcgat
    47641 tttgtctgcc agcggcgaat cggcaatgtt atccagcgcc tgcaccacct gcatattgcc
    47701 ctgccccggc ggcgtccagt cgagttgagt cacttctaca tgctgctttt tcaggtcatc
    47761 gctaaacatg gcgataccga cgttaataac gttcaacggt tggctaaaca gtgactggct
    47821 catcaggctt cctccccttt gcagataaat tcacgcgcca gcaatccggt attggtgctg
    47881 ctgctcgcca gaatcactcc ggcatcaagc agcatctggc tttgttgttc taatgatggc
    47941 gtatcgagat cggtgcccag cacataggcg agaatgatca actcgcgtcc ttcggcggcg
    48001 gcgatcgctt tcgcttcttt gatcgcctcg atggtggagc cgaccggatc ttcatgcgat
    48061 ccaaatccga gcacaaaatc catcacgatc accgccactt ctggatcgcg cgcttcttcg
    48121 atcaagcgac tgatgcggtt ggtggggtca atcattggat gcggcttgcc gttggtgaag
    48181 tcgtcatcgc caaagtcgag gaaggtgtgt ttgatgctgc ggttgatatc ttgcaggcgg
    48241 aattcaggat cgggctgaat gttgctgtag acatcgccat gtttttccat caccgcgaac
    48301 atggtttcgt cgcacagcgt gccgccgcag aacaggccac gaatgtattt ctgctgcggt
    48361 tgcagacgcg cacgcacatc agcaatcaac ggctggttaa gcgtatgcag gtcgagattt
    48421 tcctgtttca cgccggagag catcaccgct tttagcgccg cctctttgct gccgcgggca
    48481 aactgtagcc cctgctcatc caccggcgtt tcaacgcgac cgaggaagca gaccaccacc
    48541 ggcttgcggc aggcacgtgc gcgttccagc actttgcggg ccaccgcagg cgcgggaggt
    48601 tttgaaatca gtgcgataat ttcagtttgc ggatcgtttt ccagcatccc gatggcgtcg
    48661 agcatcatca ggccgccgat tttctcgctc aggtcgcgcc cgccggtgcc aatcagttgt
    48721 gaaacaccgc caccaaattc atgaatgcgg acgctcagct cctggctgcc ggtgccggat
    48781 gcgccaacaa taccgatatt gccgcgacgc acggcgttac caaaacagag cgccgcgccg
    48841 ttaataatcg ccgtgccgca gtccggcccc atcatcagca gccctttttc gtgggcaaac
    48901 tgcttgagcg ccagttcatc ttcaactgag acgttatcgg aaaacagcat cacgttaagg
    48961 tcgttttgca gcgcctgacg cgcttcgcga gcggcaaaca gaccgttaac cgaaatcacc
    49021 gccaggttac tttccgggat atgctttttg gcgctggcaa tagtggcgta acgcgcctcg
    49081 tgcgagccgc tttgcgcttt gctgttgaac agttcttcaa tctccaccag caactgttcg
    49141 ttgtccgcgc ccgctttgcc gttgatgaca atcatcaggt cgccgttttt cgcctgttcc
    49201 agctccggcg tcagcagtcc taaatttttc agtacacctt tgttcatttc ggtcgccatc
    49261 gccacaaatg cctgctcgac gccgtcgagt ttatttgcac gcgtggagat agacatcagc
    49321 gagacagaat caaaataggt attcggttta atgactattt taactgacat aattccctcc
    49381 gtaggtttgg ctcagcgcac aaccatcggc catgccatac agcatgtcgc agccggaact
    49441 ggagccaata tgtttaattt tttctattgc ctgagttgcg ttcccaggga tgtcataaat
    49501 aatgttgtga ataaaacgat gaatattttc ccggcaacgt tgttgtaatg cggcttccag
    49561 cgttatagca cttaataatg tggtgttatt tctgccgcgc tgcataccga gataaaattc
    49621 ctctttgtat ttttccgacg gatgcccggg aataaataaa ataagcgcca gacctgttaa
    49681 ataatcgtcg gctgatggcg taaggccaat ccctaaccca atcatactgg cgaccgctgc
    49741 ggcgatattt tgtttttcgt ttactgcctg aataactgcc cgtcgtttaa tatgtagttc
    49801 tttatttaac gcctgataaa acggattatc gccgcgataa ttaaacaggg tttcattttc
    49861 ccccaggtgc tgatgaataa tgtcgtgcca ctgtagccag gcgataaggt gaaaattcac
    49921 agcggtcatt tgcaaggttg gggacagcca acgccgacaa cgactcatct ctatatgaag
    49981 atgttgacca accgtaatac cttgatcgtc aaactgaacc ttatcaccat gccggaacag
    50041 atcatcaaag tgagtgagtg ccaaccgaca actgtttggc gcattgtcgt actcttcaca
    50101 aagcagcgtc agcaactgct ggcgagccgg gagtgcgata tttaccgctc tggcaaagac
    50161 ctgttcaacc tgtccttgcc cccgctgcgc cagaaaatcc gcatccgcag ttagcgccca
    50221 cacacaccgt ctttctggtg tgttgatagc aagcctcccg gtttatgcac ctgcggcaat
    50281 cagtaactgc gcaatctctt caaagccccg ttcccgcgcc agttccagtg gcgttttgcc
    50341 atatttatcg gtcagatgcg ggctggcacc gtgttccagc aataactgca caatcgcctg
    50401 ctgtttaata ccaccatcat taagcacaat cgcttccagt agcggcgtcc agcctacatg
    50461 gttggtctgg ttaacgttaa tctccgtatg cgccaaaagc tcttttacaa tacttaaatg
    50521 gcctttttca caggcaggcg tcaggccgac accgccaaaa cgggttacgc aattaagatc
    50581 gggtttagcc ggtaaaataa ttcgtagtag cgttaaatca tcgttcagac agctaattaa
    50641 aaaaggattt aaacaggtat gatcttgctt attaatatcc gctccggcat caattaatgc
    50701 ctgaacgcaa gcatattgct gatataaact tgccagcgta attgccgttt tcccctgacg
    50761 atcgcaggta ttaatatcga cacccagcgc aaggcaggtt tttactttat cgatatcacc
    50821 ctgttgtgca gccaataaat agtcggcagg tagatttttt atactcattc acattcccct
    50881 ggtttttgtg gctgttttcg atgaattgag aatagcaacg ataatctgat aaaagaaccc
    50941 gcttaaattt aattcgctgt atttcgcctt tttgttgaat gatgttcaac aatcttttcg
    51001 cagtgtgcgg tagtgcaata tttgtttctt tgacccgata ccctttcact caatcaggtt
    51061 ttatttctct atagtctgca catgtaagag tagctttcgt ccatgtctaa ccagagggga
    51121 acaagatgaa cggactcacg gcaacgggtg tcaccgtcgg tatttgtgca gggctttggc
    51181 agttagtgtc ttcacatgtc gggctctctc agggatggga attattagga accattggct
    51241 tcgtcgcctt ttgcagtttt tatgccgcag gcggcggtaa atcggggttt atcagaagtc
    51301 tggcggttaa ttattccggg atggtgtggg catttttcgc cgcgctggct gccggttggc
    51361 tggcctctgt aagtggactg tccgcgttct gggcgagcgt aattactacc gtacccttct
    51421 cggcggtagt ggtctggcaa gggcgtttct ggttactgtc ctttattccc ggtggctttc
    51481 tcggcatgac gctgtttttt gccagcggga tgaactggac ggtgacgtta ctgggttttc
    51541 tggcgggtaa ctgcgtgggg ataatttcag aatatggtgg acaaaaatta agcgaagcga
    51601 cgactaaacg cgacgggtat taaaagttat tcaaaaatga ttatctatct ttaaccgata
    51661 cctctacagt catagctgga gaaattcata gtagataatg gcgagaatgg tttaatgaac
    51721 tcaattttta ccgaagagaa tctgttggca tttactaccg ctgcgcgttt tggcagcttc
    51781 agtaaagccg cagaagagtt gggtttaacc acttccgcca ttagctacac catcaagcgt
    51841 atggagacgg ggctggatgt ggtgctgttc actcgcagta cccgcagcat tgagttaacg
    51901 gagtccgggc gttatttttt ccgcaaagct accgacctgc tgaatgattt tcacgccatc
    51961 aaacgcagta tcgatactat ttcgcagggc atagaagcac gtgtgcgcat ttgtattaat
    52021 cagctgcttt atacgcccaa acacaccgca cgcttgttat aggtgctgaa aaagcagttt
    52081 cctacctgcc agattaccgt caccaccgaa gtgtataacg gcgtctggga tgcgattatt
    52141 aataatcagg ccaatatcgc catcggcgca cccgatacac tactggacgg cggcggtatt
    52201 gattacactg aaattggcgc gattcgctgg gcttttgcta tcgccccgga tcacccgtta
    52261 gcctttgtgc cggaacccat cgccgaaagc caactgcgtc tctaccctaa tattatggtg
    52321 gaggacaccg cgcatacgat taataaaaaa gtgggctggt tgctgcacgg gcaggagtca
    52381 attctggtgc cagatttcaa caccaaatgt cagtgccaga ttttaggtga aggaattggt
    52441 tttttgccgg attacatggt ccgtgaggcg atgacgcaat ccctgctggt tacccgacaa
    52501 atccataatc cgcgccagga ttcgcgcatg ttactggcga ctcagcattc tgctacgggt
    52561 caggtgacgc agtggattaa aaaacaattt gcgccaaatg gcatattaac ggggatctat
    52621 caggatttac tgcatcggga gaattaatcg ctgccaacct cccgatgcgc tgtgaatagc
    52681 ggtttacatc aaccacctgc tttcattacc cattcagaga taaatttatt acccggtact
    52741 ggcggagagt acaaatagcc ctgcaaaaag tgaacgcctt taccgatcat taaatccgcc
    52801 tgctcctggg tttctacccc ttccgccacg atactcagac caggcttacg cgctagttcg
    52861 acaatattgt ccacaatatg accggagatt tcgtcgacac tcgccatttg cacaaatgac
    52921 ttatcgatct taataaaatc gaccgggaac gcctgcaagt aacgataggt cgcataaccc
    52981 gtaccaaagt catccagcgc aaaggtaatg ttgtgctgat gaaggctgtc aaatatcgct
    53041 ctggcttctg gcgttaccgg aatagggtta cgttccgtta gctcgagaac cagcttgatt
    53101 ttatcgttac ctaatttctt aaccaggttc agacactctt tttcaaatcc cgctgccaaa
    53161 aaacaacccg ccgagacgtt gatgccaata tggaaattgt ccggcagcaa atgttttacc
    53221 ggcatcagaa tatccgcagt ctgtttcatc agttggcggg tcattatgac aataagaccg
    53281 gatgactccg ccagaggaat aaactgatcc ggtgggataa ttcccgtttg tggatgttcc
    53341 cagcggacaa gtacctcaca gcccgtcagt accccagtct gcgcgcagaa aaccggttgg
    53401 atccacggtt tgaattcgtg gttttccaac gccagactga tggcttcagg cgtgacgata
    53461 tggtgacaac ggttatcagg gatataaaaa ttatttttat ttcccgtgcc actgatgact
    53521 tgcggatgat attgaaaaaa gatatcccgc cagaaggtaa tatcactctg gatgcctagt
    53581 ttttcataca gctgcttctt ttgatgtgaa attgtctttg cactgcgatt tctgtactgt
    53641 gagatttctg taaccgacat tccctccatt agcagcttga gtaaaagccg ttctttttcg
    53701 gacacgatac caggcaacgt caaattcacc gttgccccga actcttccaa aaaaacactc
    53761 aaatcatgcg aagccattga acacaccttg attaaatctt gtgcgccagc taaatctgcg
    53821 agtcttcact ctgactgata aagctggctg caagaaaatg atacagagag ccgaattatc
    53881 tcccgttcgg ctctctctcc ttttactcat tattcagatc agaattgcca cttaatgcca
    53941 accattgcag aggtgtcatt atatccccta tcggcaacct gaacgcccac attcccccag
    54001 acattcaggt tggcatttag ctgtccttcc accccggttt ttatctcagc aatatttcgg
    54061 gctccatcct gagtgacgga cacgccatcc atactggtgc tgaaatcctt actgttatgc
    54121 agccagttca cttctacaaa cggctggaac tcgcgggatt taccgtcatc cattttatgg
    54181 tggctcttca gccaggtttt tacgccaagt cgggtttgaa cattgccatc accgttgcta
    54241 tgaacgaggg ttccgttgct ttcgcggtgc ttatcggctt tgactcccat ccaggtaacc
    54301 tgtgcttgcg gctgaacata ccattcatta cgcgttccct ggctgccatt aaattcagct
    54361 aatttgtgtt tgtatccagc ttccagtgaa gcggtaaatc cttttgattt ataggattca
    54421 ctttgtaagt catccccttt cactgtgtta tcaaaccagc tgtactgcgc ccaactgtcg
    54481 agatacgcgc cattacgcga ctcgtcatcg gcataccagg tggcatagag gccggtgcta
    54541 tatccgttca cactcgcttt tgcacaataa ccggttcgcg aggaaatggt tttgctgtcg
    54601 ctgttgccat atcccgccat gaccccaaca tgccagctgt cgctgccgtt ttggctccac
    54661 tgcgcgacat cgcctcccag ttgcagaaca tagcgattgc tttgggtttt cagctggccg
    54721 ctgccatcac gccatttatt atgaccacct tcatggcgca tccacatagt ggtttgtttc
    54781 tgctcacccg tcaccatgtc ggtatagtac gtattaccca gacgctcatg cagacgcgtg
    54841 gtgaacattg tattcgctgc tgcaaggttc gcaatgtagc tacccgcctc cggtcgcagg
    54901 tcattatcca cattcagatc cggacccggc gtaggtgtcg ggttagggtt cggctctgga
    54961 ttcgggtttg gctccggatt cggcatcggg tctggctccg gctgcagttc aggagaatca
    55021 ctgccgctgg tcagatacca gttaccacta tttgcccctt gtccacgcgc gagagtgtag
    55081 tcatacgccc ccgcaacgat acgaccagcc tgaacaaatt cgccctcaga cttaccgtct
    55141 acatggataa gttcgatacc gttaagggtt ttcgcacctg taccgccagc gttattcacc
    55201 gtaacggcag tcgtgccgct agtatcgccc tcgacaacca atttatcggt tactgagtca
    55261 tcgccattca gtaccgtatt cattactagt tgaccgttat taccgtggta gtaccccttc
    55321 accactaaca cattgccagc ttgctgtcct gacattccgg tcatcagcgt accgttgttg
    55381 gtcagattgc cgcccagggt gtaattcaca gacgcagccg ctcttttttc agcgctatta
    55441 ttcaacgcca gggtaccgtt gttctcaacg tttgcgctac ccaatccctg cgcatgactt
    55501 accaggacat tagccgaatt agcaatgttc acattcgccg tcaggttgct gttatcgccc
    55561 gcgatgtcca gcgtgccgcc ctgaatattc agctctccgc tccccgttaa cgaaccggtt
    55621 gaagtgcccc cgttggtaac cgtcagactg ccgccattta agctcagcag tgaatcggcg
    55681 ctgccattga gcgtgcccac ggtctggctg tgaccattca tatccagttc agtctctgcc
    55741 gccagacgca gttcatgggt attacccaac acgttatcgt tattcaacaa aagcgtccca
    55801 ctgcgcagat cggtaacacc ggtatagtcg ttgtctttgt tagaaagcga tacggtctga
    55861 cccttctggc tgctgaatgc caggtcacca ctgccggtaa tctttgcgct gagatcggcg
    55921 gcattctcgc ttttaccgtt agagctcagc accagagcat cgctgtcggt agcgtgtaaa
    55981 tcaagctggg tcaggccgta accgatatac agaccgtcat tgtttacccc actcgtcaga
    56041 cgatagtcgt aagtgccgac ggccgctttt tgcccattct gctgaatatc acgttgggca
    56101 ctgctggaga ggatttgccc atttttatcc tgcaattgca gattgcccgc atcgcccaga
    56161 acttccgcac cttgcgcatc aaccaactta atggtgctgt taccatcatc gacttcagtg
    56221 agcgataacg cagagtcaat atcacgagat actgagctta caacgtcact gtcagaaacc
    56281 tgaatcacac cctcgccgcg caaatccagc gttttactaa cgttgaccgt cccttctgtc
    56341 atctgtgcac ctgctgtcag ggcaccaaaa tcaacggttc cgccggagaa tcccaaagtg
    56401 ctaatggagt gagcgccctt acccactgtt acctggcttc cctgacccgc agtgatcccg
    56461 cctctctgca gaaactcagc attcttttcc aggttaaatg tcgtgttctg gaacgccagt
    56521 gaaccctgga accagtctgt ataagcaaac tcatcgaagg agaattcgtg attccccatg
    56581 tttatttcaa caatacctct accttgaaga tcgttatcaa atacccaatc tttattggca
    56641 ttgatttgca gtttgccatc aacatccagg tccgcacggc cgatgttctt gtcttcagaa
    56701 ataaccgcga cagcaccaga agcgacatca atatatccgc tgaatgcata gttattgccg
    56761 gtcagtgcca ctgtcccact gtcgatactt attgtaccat tgccggaaat cttattggca
    56821 aaggtcatgc tatcgtcagc cttcaggtgt aatttcccgc ggttgttgat ctctgctgta
    56881 cctatatttt tggtgatccc taaagcgcca ttttgctcaa ttttgacttt accactaaag
    56941 gcagagttat caccggttaa gtaagtcgtg ccattgagcg cattgatata gcccaggccc
    57001 tcgctgccac tgacaatatc cgcggaaata gtcgcgtttt tatccgtgtg gtttaaggtg
    57061 attttcttat cggatgtctt tgcccacaca aactcaattt tttcggcatc aataatccct
    57121 gcagccttcg cagcacttcc ttcctgtgca cctaacgcta actcagagtt ggtgcttaaa
    57181 ctaattttcg gtgcggatat tttacctcca tctgatacga ctaatgttgt atcagaacgt
    57241 tggtcgtaat aacccaggct aatatgttca cgagcattaa cttcaccacc gttattaacc
    57301 gacaggaact gattaccgcc atatccgccg taaacgccca taaagaaggt ccctgcattt
    57361 acctgactac cttcgccgct tacattcccc tgcgtataag tcagacgact ggtgccctta
    57421 acatgaattt tgccgccatt ggagacatta aaatagcctt gatcatgtct catgcctgag
    57481 tttgactcat acaaatcatc agcatataac tcgctgccag cattggagac attcaggaac
    57541 cccgtacctt tcagcaaaaa gttaagcggg ccattgattg ttacctttcc tccggtaata
    57601 tccaacccac caccagaagc gagagtcaga cgcggacggg aagtatctgt cgtattgatt
    57661 accagattgg cattgtcagt caccgtaata cggtaatcga tgctctggtt ctctttgcca
    57721 aaatcaatag ctttatcctg gtttacgatg acatcatcgg cgaataccag agatgaatat
    57781 aaacttatcg cgctaattaa aacagcccgg gacttttttc cttttactct gctccccagc
    57841 tccgagcaga cgctccaaca gttacgggac ctgttccact ttagccgata tattttattc
    57901 atcacaatct ccagcaatac atccaaataa aaccaagatt gcataaacag caacgcatta
    57961 aatgccattc tggttactgt tcatcgcata taaaaattca aaaattttgc atagcattgg
    58021 caaccacata tctgagtatg tagttcagag ggtaataaaa tagcaagtta ctactgaaaa
    58081 cttattttaa gttaacactc tt
//
LOCUS       DAPGWD010000012        58055 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPGWD010000012 DAPGWD010000000
VERSION     DAPGWD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.012"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            121..2655
                     /gene="mrcB"
                     /locus_tag="RHX88_000685"
     CDS             121..2655
                     /gene="mrcB"
                     /locus_tag="RHX88_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDV2229416.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2875..5118
                     /gene="fhuA"
                     /locus_tag="RHX88_000686"
     CDS             2875..5118
                     /gene="fhuA"
                     /locus_tag="RHX88_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDV2229417.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5169..5966
                     /gene="fhuC"
                     /locus_tag="RHX88_000687"
     CDS             5169..5966
                     /gene="fhuC"
                     /locus_tag="RHX88_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDV2229418.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSCCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5966..6856
                     /gene="fhuD"
                     /locus_tag="RHX88_000688"
     CDS             5966..6856
                     /gene="fhuD"
                     /locus_tag="RHX88_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDV2229419.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6853..8835
                     /gene="fhuB"
                     /locus_tag="RHX88_000689"
     CDS             6853..8835
                     /gene="fhuB"
                     /locus_tag="RHX88_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDV2229420.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFVLAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8870..10150)
                     /gene="hemL"
                     /locus_tag="RHX88_000690"
     CDS             complement(8870..10150)
                     /gene="hemL"
                     /locus_tag="RHX88_000690"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDV2229421.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10375..11796
                     /gene="clcA"
                     /locus_tag="RHX88_000691"
     CDS             10375..11796
                     /gene="clcA"
                     /locus_tag="RHX88_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDV2229422.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11820..11885
                     /locus_tag="RHX88_000692"
     CDS             11820..11885
                     /locus_tag="RHX88_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229423.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11878..12222
                     /gene="erpA"
                     /locus_tag="RHX88_000693"
     CDS             11878..12222
                     /gene="erpA"
                     /locus_tag="RHX88_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDV2229424.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12269..12892)
                     /locus_tag="RHX88_000694"
     CDS             complement(12269..12892)
                     /locus_tag="RHX88_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDV2229425.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12930..13730)
                     /gene="btuF"
                     /locus_tag="RHX88_000695"
     CDS             complement(12930..13730)
                     /gene="btuF"
                     /locus_tag="RHX88_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDV2229426.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13723..14421)
                     /gene="mtnN"
                     /locus_tag="RHX88_000696"
     CDS             complement(13723..14421)
                     /gene="mtnN"
                     /locus_tag="RHX88_000696"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDV2229427.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14505..16022
                     /gene="dgt"
                     /locus_tag="RHX88_000697"
     CDS             14505..16022
                     /gene="dgt"
                     /locus_tag="RHX88_000697"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDV2229428.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16152..17576
                     /gene="degP"
                     /locus_tag="RHX88_000698"
     CDS             16152..17576
                     /gene="degP"
                     /locus_tag="RHX88_000698"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDV2229429.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            17731..18888
                     /gene="cdaR"
                     /locus_tag="RHX88_000699"
     CDS             17731..18888
                     /gene="cdaR"
                     /locus_tag="RHX88_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDV2229430.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(18977..19363)
                     /locus_tag="RHX88_000700"
     CDS             complement(18977..19363)
                     /locus_tag="RHX88_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDV2229431.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19678..20502)
                     /gene="dapD"
                     /locus_tag="RHX88_000701"
     CDS             complement(19678..20502)
                     /gene="dapD"
                     /locus_tag="RHX88_000701"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDV2229432.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20533..23205)
                     /gene="glnD"
                     /locus_tag="RHX88_000702"
     CDS             complement(20533..23205)
                     /gene="glnD"
                     /locus_tag="RHX88_000702"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDV2229433.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23267..24061)
                     /gene="map"
                     /locus_tag="RHX88_000703"
     CDS             complement(23267..24061)
                     /gene="map"
                     /locus_tag="RHX88_000703"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDV2229434.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24429..25154
                     /gene="rpsB"
                     /locus_tag="RHX88_000704"
     CDS             24429..25154
                     /gene="rpsB"
                     /locus_tag="RHX88_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDV2229435.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25412..26263
                     /gene="tsf"
                     /locus_tag="RHX88_000705"
     CDS             25412..26263
                     /gene="tsf"
                     /locus_tag="RHX88_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDV2229436.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26410..27135
                     /gene="pyrH"
                     /locus_tag="RHX88_000706"
     CDS             26410..27135
                     /gene="pyrH"
                     /locus_tag="RHX88_000706"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDV2229437.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27427..27984
                     /gene="frr"
                     /locus_tag="RHX88_000707"
     CDS             27427..27984
                     /gene="frr"
                     /locus_tag="RHX88_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDV2229438.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28076..29272
                     /gene="ispC"
                     /locus_tag="RHX88_000708"
     CDS             28076..29272
                     /gene="ispC"
                     /locus_tag="RHX88_000708"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDV2229439.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            29461..30219
                     /gene="ispU"
                     /locus_tag="RHX88_000709"
                     /gene_synonym="uppS"
     CDS             29461..30219
                     /gene="ispU"
                     /locus_tag="RHX88_000709"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDV2229440.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30232..31089
                     /gene="cdsA"
                     /locus_tag="RHX88_000710"
     CDS             30232..31089
                     /gene="cdsA"
                     /locus_tag="RHX88_000710"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV2229441.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31101..32453
                     /gene="rseP"
                     /locus_tag="RHX88_000711"
     CDS             31101..32453
                     /gene="rseP"
                     /locus_tag="RHX88_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDV2229442.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAILAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32483..34915
                     /gene="bamA"
                     /locus_tag="RHX88_000712"
     CDS             32483..34915
                     /gene="bamA"
                     /locus_tag="RHX88_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDV2229443.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAIASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35037..35522
                     /gene="skp"
                     /locus_tag="RHX88_000713"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35037..35522
                     /gene="skp"
                     /locus_tag="RHX88_000713"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDV2229444.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35526..36551
                     /gene="lpxD"
                     /locus_tag="RHX88_000714"
     CDS             35526..36551
                     /gene="lpxD"
                     /locus_tag="RHX88_000714"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDV2229445.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36656..37111
                     /gene="fabZ"
                     /locus_tag="RHX88_000715"
     CDS             36656..37111
                     /gene="fabZ"
                     /locus_tag="RHX88_000715"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDV2229446.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37115..37903
                     /gene="lpxA"
                     /locus_tag="RHX88_000716"
     CDS             37115..37903
                     /gene="lpxA"
                     /locus_tag="RHX88_000716"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDV2229447.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYIIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37903..39051
                     /gene="lpxB"
                     /locus_tag="RHX88_000717"
     CDS             37903..39051
                     /gene="lpxB"
                     /locus_tag="RHX88_000717"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDV2229448.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39048..39644
                     /gene="rnhB"
                     /locus_tag="RHX88_000718"
     CDS             39048..39644
                     /gene="rnhB"
                     /locus_tag="RHX88_000718"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDV2229449.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            39681..43163
                     /gene="dnaE"
                     /locus_tag="RHX88_000719"
     CDS             39681..43163
                     /gene="dnaE"
                     /locus_tag="RHX88_000719"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDV2229450.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43176..44135
                     /gene="accA"
                     /locus_tag="RHX88_000720"
     CDS             43176..44135
                     /gene="accA"
                     /locus_tag="RHX88_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDV2229451.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44234..46375
                     /gene="ldcC"
                     /locus_tag="RHX88_000721"
     CDS             44234..46375
                     /gene="ldcC"
                     /locus_tag="RHX88_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDV2229452.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46432..46821
                     /locus_tag="RHX88_000722"
     CDS             46432..46821
                     /locus_tag="RHX88_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV2229453.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46886..48184
                     /gene="tilS"
                     /locus_tag="RHX88_000723"
     CDS             46886..48184
                     /gene="tilS"
                     /locus_tag="RHX88_000723"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDV2229454.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48233..48493)
                     /gene="rof"
                     /locus_tag="RHX88_000724"
     CDS             complement(48233..48493)
                     /gene="rof"
                     /locus_tag="RHX88_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDV2229455.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48480..48680)
                     /locus_tag="RHX88_000725"
     CDS             complement(48480..48680)
                     /locus_tag="RHX88_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDV2229456.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48846..49391
                     /locus_tag="RHX88_000726"
     CDS             48846..49391
                     /locus_tag="RHX88_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDV2229457.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49388..49810
                     /gene="arfB"
                     /locus_tag="RHX88_000727"
     CDS             49388..49810
                     /gene="arfB"
                     /locus_tag="RHX88_000727"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV2229458.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49824..50534
                     /gene="nlpE"
                     /locus_tag="RHX88_000728"
                     /gene_synonym="cutF"
     CDS             49824..50534
                     /gene="nlpE"
                     /locus_tag="RHX88_000728"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDV2229459.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(50689..51512)
                     /locus_tag="RHX88_000729"
                     /pseudo
     CDS             complement(50689..51512)
                     /locus_tag="RHX88_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(51566..53284)
                     /gene="proS"
                     /locus_tag="RHX88_000730"
     CDS             complement(51566..53284)
                     /gene="proS"
                     /locus_tag="RHX88_000730"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDV2229460.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(53396..54103)
                     /gene="tsaA"
                     /locus_tag="RHX88_000731"
     CDS             complement(53396..54103)
                     /gene="tsaA"
                     /locus_tag="RHX88_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDV2229461.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(54100..54504)
                     /gene="rcsF"
                     /locus_tag="RHX88_000732"
     CDS             complement(54100..54504)
                     /gene="rcsF"
                     /locus_tag="RHX88_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDV2229462.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(54622..55437)
                     /gene="metQ"
                     /locus_tag="RHX88_000733"
     CDS             complement(54622..55437)
                     /gene="metQ"
                     /locus_tag="RHX88_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDV2229463.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(55477..56130)
                     /gene="metI"
                     /locus_tag="RHX88_000734"
     CDS             complement(55477..56130)
                     /gene="metI"
                     /locus_tag="RHX88_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDV2229464.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(56123..57154)
                     /gene="metN"
                     /locus_tag="RHX88_000735"
     CDS             complement(56123..57154)
                     /gene="metN"
                     /locus_tag="RHX88_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDV2229465.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            57342..57914
                     /gene="gmhB"
                     /locus_tag="RHX88_000736"
     CDS             57342..57914
                     /gene="gmhB"
                     /locus_tag="RHX88_000736"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDV2229466.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        13766 a        14393 c        15974 g        13922 t
ORIGIN      
        1 ccctctccct caagggagag gggaccgatc gagcacaaat tttgagagat atcttcttct
       61 gtcttgtaac agaagaacag aaaatcgggc ttttgcgcct gaatattgcg gagaaaaagc
      121 atggccggga atgaccgcga gccaattgga cgcaaaggga aaccgacgcg tccggtcaaa
      181 caaaaggtaa gccgtcgtcg ttacgaagat gacgatgatt acgacgatta tgatgactat
      241 gaggatgaag aaccgatgcc gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt
      301 ggcaaacgcg gctggctatg gctactgcta aaactggcta tcgtttttgc cgtgctgatc
      361 gccatttacg gcgtttatct cgatcaaaaa attcgtagcc gtattgatgg caaggtctgg
      421 caactgcctg cggcagttta tggccgaatg gtcaaccttg agccagacat gaccatcagc
      481 aagaacgaga tggtgaagct gctggaggcg acccagtatc gccaggtgtc gaaaatgacc
      541 cgtcctggcg aatttaccgt gcaggccaac agcattgaga tgatccgccg tccgtttgat
      601 ttcccggaca gtaaagaagg gcaggtgcgt gcgcgtctga cctttgatgg cgatcatctg
      661 gcgacgatcg tcaatatgga gaacaaccgt cagttcggtt tcttccgtct tgatccgcgc
      721 ctgatcacca tgatctcttc gccaaacggt gagcagcgtc tgtttgtgcc gcgcagtggt
      781 ttccctgatt tgctggtgga tactttgctg gcgacagaag accgccattt ctacgagcat
      841 gatgggatca gcctctactc aattgggcgt gcggtgctgg caaacctgac cgcaggtcgc
      901 acggtgcagg gtgcgagtac gctgacgcaa cagctggtga aaaacctgtt cctctccagc
      961 gagcgctctt actggcgtaa agcgaacgaa gcctacatgg cgctgatcat ggacgcgcgt
     1021 tacagcaaag accgtattct tgagctgtat atgaacgagg tgtatctcgg tcagagcggc
     1081 gacaacgaaa tccgcggctt cccgctggca agcttgtatt actttggtcg cccggtagaa
     1141 gagctaagcc tcgaccagca ggcgctgtta gtcggtatgg tgaaaggggc gtccatctac
     1201 aacccgtggc gtaacccgaa actggcgctg gagcgacgta atctggtgct gcgtctgctg
     1261 caacagcaac agattattga tcaagaactc tatgacatgt tgagtgcacg tccgctgggg
     1321 gttcagccgc gcggtggggt aatctctcca cagccagcct ttatgcaact ggtgcgtcag
     1381 gagctgcagg caaaactggg cgataaggta aaagatctct ccggcgtgaa gatcttcact
     1441 acctttgact cggtggccca ggacgcggca gaaaaagccg ccgtggaagg cattccggca
     1501 ctgaagaaac agcgtaagtt gagcgatctt gaaactgcga ttgtggtcgt cgaccgcttt
     1561 agtggtgaag ttcgtgcgat ggtcggaggt tctgagccgc agtttgcggg ctacaaccgt
     1621 gcgatgcagg cgcgtcgttc gattggttcc cttgcaaaac cagcgactta tctgacggct
     1681 ttaagccagc cgaaaatcta tcgcctgaac acgtggattg ccgatgcgcc aatcgcgctg
     1741 cgtcagccga atggccaggt ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc
     1801 ggcagagtga tgctggtgga tgcgttgacc cgttcgatga acgtgccgac ggtaaatctg
     1861 gggatggcgc tggggctgcc tgcagttacg gatacctgga tcaaactggg cgtaccgaaa
     1921 gatcagctgc atccggttcc ggcgatgctg ctgggggcgt tgaacttaac gccaatcgaa
     1981 gtggcgcagg cgttccagac catcgccagc ggtggtaacc gtgcaccgct ttctgcactg
     2041 cgttcggtaa tcgcggaaga tggcaaggtg ctgtatcaga gcttcccgca ggcagaacgc
     2101 gctgttccgg cgcaggcggc gtatctgaca ctatggacca tgcagcaggt ggtacaacgt
     2161 ggtacgggtc gtcagcttgg ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg
     2221 actaccaaca ataacgtaga tacctggttt gcaggcattg acggcagcac ggtgaccatc
     2281 acctgggtag gccgtgataa caaccagccg accaaactgt atggagccag cggggcaatg
     2341 tcgatttatc agcgttatct ggctaaccag acgccaacgc cgctgaatct tgttccgcca
     2401 gaagatattg cagatatggg cgtggactac gacggcaact ttgtttgcag cggtggcatg
     2461 cgtgtcttgc cggtctggac cagcgatccg caatcgctgt gccagcagag cgagatgcag
     2521 cagcagccgt caggcaatcc gtttgatcag tcttctcagc cgcagcaaca gccgcaacag
     2581 caacctgctc agcaagagca gaaagacagc gacggtgtag ccggttggat caaggatatg
     2641 tttggtagta attaacatct aagcgtgaaa taccggatgg cgagttgcca tccggtaaaa
     2701 taacatccca tctaagatat taaccctttc ttttcatctg gttgtttatt aacccttcag
     2761 gaacgctcag attgcgtacc gcttgcgaac ccgccagcgt ttcgaatatt atcttatctt
     2821 tataataatc attctcgttt acgttatcat tcactttaca tcagagatat accaatggcg
     2881 cgttccaaaa ctgctcagcc aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca
     2941 gcggtaagcg gcatgtctgt ttatgcacag gcagcggttg aaccgaaaga agacactatc
     3001 accgttaccg ctgcacctgc gccgcaagaa agcgcatggg ggcctgctgc aactattgcg
     3061 gcgcgacagt ctgctaccgg cactaaaacc gatacgccga ttcaaaaagt gccacagtct
     3121 atttctgttg tgaccgccga agagatggcg ctgcatcagc cgaagtcggt aaaagaagcg
     3181 cttagctaca cgccgggtgt ctctgttggt acgcgtggcg catccaacac ctatgaccac
     3241 ctgatcattc gcggctttgc ggcagaaggc caaagccaga ataactatct gaatggcctg
     3301 aagttgcagg gcaacttcta taacgatgcg gtcattgacc cgtatatgct ggaacgcgct
     3361 gaaattatgc gtggcccggt ttccgtgctt tacggtaaaa gcagtcctgg cggcctgttg
     3421 aatatggtca gcaagcgtcc gaccaccgaa ccgctgaaag aagttcagtt taaagccggt
     3481 actgacagcc tgttccagac tggttttgac tttagcgatt cgttggatga tgacggtgtt
     3541 tactcttatc gcctgaccgg tcttgcgcgt tctgccaatg cccagcagaa agggtcagaa
     3601 gagcagcgtt atgctattgc accggcgttc acctggcgtc cggatgataa aaccaatttc
     3661 accttccttt cttacttcca gaacgagccg gaaaccggtt attacggctg gttgccgaaa
     3721 gagggaaccg ttgagccgct gccgaacggt aagcgtctgc cgacagactt taatgaaggg
     3781 gcgaagaaca acacctattc tcgtaatgag aagatggtcg gctacagctt cgatcacgaa
     3841 tttaacgaca cctttactgt gcgtcagaac ctgcgctttg ctgaaaacaa aacctcgcaa
     3901 aacagcgttt atggttacgg cgtctgctcc gatccggcga atgcttacag caaacagtgt
     3961 gcggcattag cgccagcgga taaaggccat tatctggcac gtaaatacgt cgttgatgat
     4021 gagaagctgc aaaacttctc cgttgatacc cagttgcaga gcaagtttgc cactggcgat
     4081 atcgaccaca ccctgctgac cggtgtcgac tttatgcgta tgcgtaatga catcaacgcc
     4141 tggtttggtt acgacgactc tgtgccactg ctcaatctgt acaatccggt gaataccgat
     4201 ttcgacttca atgccaaaga tccggcaaac tccggccctt accgcattct gaataaacag
     4261 aaacaaacgg gcgtttatgt tcaggatcag gcgcagtggg ataaagtgct ggtcaccctg
     4321 ggcggtcgtt atgactgggc agatcaagaa tctcttaacc gcgttgctgg gacgaccgat
     4381 aaacgtgatg acaaacagtt tacctggcgt ggtggtgtta actacctgtt tgataatggt
     4441 gtaacacctt acttcagcta tagcgaatcg tttgaacctt cttcgcaagt tgggaaggat
     4501 ggtaatattt tcgcaccgtc taaaggtaag cagtatgaag tcggcgtgaa atatgtaccg
     4561 gaagatcgtc cgattgtagt tactggtgcc gtgtataatc tcactaaaac caacaacctg
     4621 atggcggacc ctgagggttc cttcttctcg gttgaaggtg gcgagatccg cgcacgtggc
     4681 gtagaaatcg aagcgaaagc ggcgctgtcg gcgagtgtta acgtagtcgg ttcttatact
     4741 tacaccgatg cggaatacac caccgatact acctataaag gcaatacgcc tgcacaggtg
     4801 ccaaaacaca tggcttcgct gtgggctgac tataccttct ttgacggtcc gctttcaggt
     4861 ctgacgctgg gcaccggtgg tcgttatact ggctccagct atggtgatcc ggctaactcc
     4921 tttaaagtgg gaagttatac ggtcgtggat gcgttagtgc gttatgatct ggcgcgagtc
     4981 ggcatggcgg gctccaacgt ggcgctgcat gttaacaacc tgttcgatcg tgaatacgtc
     5041 gccagctgct ttaacactta tggctgcttc tggggcgcag aacgtcaggt cgttgcaacc
     5101 gcaaccttcc gtttctaatt tctcttttgg ggcacggatt tccgtgccca tttcacaagt
     5161 tggctgttat gcaggaatac acgaatcatt ccgataccac ttttgcactg cgtaatatct
     5221 cctttcgtgt gcccgggcgc acgcttttgc atccgctgtc gttaaccttt cctgccggga
     5281 aagtgaccgg tctgattggt cacaacggtt ctggtaaatc cactctgctc aaaatgcttg
     5341 gccgtcatca gcctccgtca gaaggggaga ttcttcttga tgcccagccg ctggaaagct
     5401 ggagcagcaa agcgtttgcc cgcaaagtgg cttatttgcc gcagcagctt cctccggcag
     5461 aagggatgac cgtgcgtgaa ctggtggcga ttggtcgtta cccgtggcat ggcgcgttgg
     5521 ggcgctttgg ggcggcagat cgcgaaaaag tcgaggaagc tatctcgctg gttggcttaa
     5581 aaccgctggc gcatcggctg gtcgatagtc tctctggcgg cgaacgtcaa cgggcgtgga
     5641 tcgccatgct ggtggcgcag gatagctgtt gtctgttgct cgacgaaccg acttcggcgc
     5701 tggatatcgc ccaccaggtt gatgtgctgg cgctggtgca ccgtttaagt caggagcgtg
     5761 gcctgacggt cattgccgtg ttgcacgata tcaatatggc ggcacgctac tgtgattatc
     5821 tggtcgccct gcgcggtggt gaaatgattg ctcagggaac gcctgcggaa attatgcgcg
     5881 gcgaaactct cgaaatgatt tatggcatcc cgatgggtat tttgccgcat ccggcgggtg
     5941 ctgcacctgt gagttttgtt tattgatgag cggcttacct cttatttcgc gccgtcgact
     6001 gttaacggcg atggcgcttt ctccgttgtt atggcagatg aataccgccc acgcggcggc
     6061 tattgacccc aatcgtattg tggcgctgga gtggttgccg gtggaattac tgctggcgct
     6121 cggcatcgtg ccttacggcg tggcggatac catcaactat cgcctgtggg tcagcgaacc
     6181 accattgccg gactcagtga ttgacgtcgg tttgcgtaca gaacctaacc ttgaactgct
     6241 gaccgaaatg aaaccgtcgt ttatggtctg gtcggcagga tatggccctt catcagaaat
     6301 gctggcacgt attgcgccgg gtcgcggatt taacttcagc gatggcaaac agccgttggc
     6361 gatggcgcgt aaatcgctga cggaaatggc agatttactt aacctgcaaa gcgcagcgga
     6421 aacgcattta gcgcaatacg aagactttat ccgcagcatg aaaccccgct ttgtgaagcg
     6481 tggtgcacgc ccgttactgc tgacgacgct tatcgatccg cgccatatgc tggtcttcgg
     6541 tccaaacagc ttgttccagg aaattctcga tgagtacggc atcccaaatg cctggcaagg
     6601 ggaaaccaac ttctggggca gtaccgccgt cagtatcgat cgtctggcgg cgtataaaga
     6661 cgttgatgtg ctctgttttg atcacgacaa cagcaaagac atggatgcgc taatggcaac
     6721 cccgctgtgg caggccatgc cgtttgtccg cgccggacgc tttcagcgcg tacccgcagt
     6781 ctggttttat ggtgcgacgc tctcggcaat gcactttgtg cgcgttctgg ataacgccat
     6841 cggaggtaaa gcgtgagtaa acgaattgcg ctttttccgg cgttattgct ggcgctgctg
     6901 gtgattgtcg ctacggcgct gacctggatg aacttttcgc aggcgctgcc gcgcagtcag
     6961 tgggcgcagg ccgcctggtc gccggatatt aacgtcatcg agcagatgat ttttcactac
     7021 agcttgttac cacgtctggc gatttctcta ctggtgggcg cgggcctggg gctggtgggc
     7081 gtgctgtttc agcaagtgct gcgtaacccg ctggcggaac cgacgacgtt gggcgttgct
     7141 acaggcgcgc aactggggat taccgtcacc acgctctggg cgatccccgg tgctatggcg
     7201 agccagtttg ccgcgctggt gggcgcttgt gtcgttggct taatcgtctt tggcgtcgcg
     7261 tgggggaaac ggctttcgcc ggttacgctt attctcgcgg ggctggtggt gagcctttat
     7321 tgcggggcaa ttaatcagct actggttatc ttccatcacg accagctgca aagcatgttc
     7381 ctgtggagca ccggaacgct gacgcaaacc gactggggcg gcgttgagcg tttatggccg
     7441 cagctgctgg gcggcgtgat gctgacgtta ctgctacttc gtccgttaac cctgatgggg
     7501 cttgatgatg gcgtggcgcg caatctcggg ctggccttat cgctcgcgcg tctggctgcg
     7561 ctgtcgctgg cgatagtcat cagtgcgctg ctggtgaatg cggtggggat tatcggcttt
     7621 atcgggttgt tcgcgccact gctggcaaaa atgctggggg cgcggcgttt gttgccacgg
     7681 ttgatgctgg cgtcgctgat tggtgcgtta attctgtggc tttctgatca aatcatcctc
     7741 tggctgactc gcgtgtggat ggaagtgtcc accggttcgg tcactgcgtt gatcggtgcg
     7801 ccgctgctac tgtggctgct gccgcgttta cggagcatta gcgcgccgga tatgaaggtc
     7861 aacgatcgtg tcgcgactga acgccaacat gtgctggcgt ttgtcctcgc gggcggcgtg
     7921 ctgctgttga tggctgtggt agtggcgctg tcgtttggtc gtgatgcgca cggctggacg
     7981 tgggcgagcg gggcgttgct tgaggatttg atgccctggc gctggccgcg aattatggcg
     8041 gcgctgtttg cgggcgtcat gctggcggtg gcgggctgta ttattcagcg gctgaccgga
     8101 aacccgatgg caagcccgga agtgctgggg attagctctg gcgcagcgtt tggcgtggtg
     8161 ttgatgctgt ttctggtgcc gggtaatgcc tttggctggc tgttacctgc aggcagtctc
     8221 ggggcggcgg tgacgctgtt gatcattatg atcgccgccg gacgcggtgg attttcccca
     8281 caccgcatgt tactggcggg gatggcgtta agtaccgcgt tcaccatgct tttgatgatg
     8341 ttgcaggcaa gtggtgaccc gcgaatggcg caagtgctga cctggatttc cggttcgacc
     8401 tacaacgcga ccgatgcgca ggtctggcgc accggaattg tgatggtgat tttgctggcg
     8461 attaccccgc tgtgccgccg ctggctgacc attttaccgc tgggtggtga taccgcccga
     8521 gccgtaggaa tggcgctaac gccgacgcga attgcgctgc tgctgttagc ggcttgcctg
     8581 acggcgaccg cgacgatgac tattggaccg ttgagttttg ttggtttaat ggcaccgcat
     8641 attgcgcgga tgatgggctt tcgacggacg atgccgcaca tcgtaatttc ggcgctggtg
     8701 ggtggtttac tgctggtgtt cgctgactgg tgtgggcgga tggtgctgtt tccattccag
     8761 atcccggcgg ggctgctgtc aacctttatc ggcgcgccat attttatcta tttgttgaga
     8821 aagcagagcc gttaattgaa tgcctcgccc ggtgggcgag gcaaaatcat cacaacttcg
     8881 caaacacccg tcgtgcagca tcgatggtgt tattgatatc ttccatgctg tgcgccacgg
     8941 acataaagcc cgcttcaaac gctgacggtg ccaggtaaac accttcgtcc agcatcatat
     9001 ggaagaaacg cttaaagcgt tccacgtcac aggccatcac atcctgatag cacgtcacgg
     9061 actcggcgtc ggtaaagaaa ataccgaaca tgccgccaac gtggttaacg accagcggaa
     9121 ttccggcttc ttctgccgct tccagcagac cttctgccag acgtgttgtc agctcatcca
     9181 gcgtttcgtg aacgcccggc tgcgcgactt cattcagaca ggcgaaaccc gctgccatcg
     9241 caattgggtt accggaaagc gtacccgcct gatagaccgg acccgtcggg gccagcgcat
     9301 ccattacatc acgacgacca ccgaatgcgc ctaccggcat tccaccgccg atgatttttc
     9361 cgaggcaggt taaatctggc actacgccgt aataatcctg tgcaccagcc agtgccacgc
     9421 ggaagccagt cattacttca tcgatgatca gcaatgcgcc aaattcgtcg cacagcgcgc
     9481 gcagacctgg caggaactct ggcagcggcg gaacacagtt catattgcct gccaccggct
     9541 caacgataat acaggcaatc tcttgcgggt attgctcaaa cgcggcgcgt acagaagcca
     9601 gatcgttata agtacaggtt aaggtatgtt tggcgaaatc tgccggaaca cccggcgagt
     9661 ttggctggcc taacgtgagt gcgccagaac cggctttcac cagcaggcag tcagcgtgac
     9721 cgtggtaaca accttcaaat ttaatgattt tgtcgcgacc ggtaaaacca cgggccaggc
     9781 ggatggcgct catggtcgct tcagtgccgg agttcaccat gcgcaccata tccatggtcg
     9841 ggaccagttc ggtcaccagt tgcgccattt tcacttccat ttcggttggt gcaccaaagc
     9901 ttaaaccacg ctcggcggct tcaatcacgg cattgcggat tgccggatgg ttatggccca
     9961 gcaccatcgg cccccaggaa ccgacataat cgatataggc tttgccatca acatcgtaca
    10021 gataagcgcc gtccgctttt tcgataaaca gtggagtgcc gcccacgcca gtaaaggcgc
    10081 gaacagggga gttcacaccg ccagggatca gctcgcgcgc tgcgctgtaa agattttcag
    10141 acttactcat ggagggttcc tgattcgtag aaaaagtgaa tggctgctat tctatgttat
    10201 tcataacaag ttaaatactc gtcaaacatc aggctgcttg tactggtgca atcctgattt
    10261 cttagagtat aaaagttttg tgcatttgaa acattacgct ttgcaaagga ttttcatgga
    10321 acgtgcgagt aaaatgccgt catcttattt gtatgaccaa taagtgatca ttggatgaaa
    10381 actgatactc cctctttaga aacaccgcag gccgcgcgcc tgcgacgcag acaactgatt
    10441 cgccaacttc ttgagcgcga taaaaccccg ttagccattt tgtttatggc ggcagtcgtc
    10501 ggcacgcttg tcgggctggc agcggttgct tttgacaaag gtgtcgcctg gttgcagaac
    10561 caacgtatgg gggcgctggt acatactgct gataattatc cgctactgtt aaccgtcgct
    10621 tttctctgtt cggcggtgct ggcgatgttc ggctactttt tggtgcgcaa atacgcgccc
    10681 gaagcagggg gatcggggat cccggaaatt gaaggggcgc tggaagatca acgtcctgtt
    10741 cgctggtggc gtgtattgcc ggtgaaattc tttggcgggc tggggacact cggcggaggc
    10801 atggtgctag ggcgcgaagg accaaccgtg cagatcggcg gcaatatcgg tcgcatggtg
    10861 cttgatattt tccgcctgaa aggtgatgaa gctcgccata cgctgctggc aaccggtgct
    10921 gctgcggggc tggctgcggc ctttaacgcg ccgctggcgg gtattttgtt tattatcgaa
    10981 gagatgcgtc cgcagtttcg ctatacgtta atttcgatta aagcggtatt tattggcgtc
    11041 attatgtcga ccattatgta ccggattttt aatcatgaag ttgcgttgat tgacgtcggt
    11101 aaactttctg atgcgccgct taatacgctg tggctttatc tgatcctcgg tattattttt
    11161 ggcattttcg gccctatttt taataaatgg gtgctgggga tgcaggattt gctgcaccgt
    11221 gtgcacggcg gcaatattac caaatgggta ctgatgggtg gtgcgattgg cggtctgtgt
    11281 ggattgctgg ggtttgtggc accagcaacg tcgggcggcg gttttaacct gattcctatc
    11341 gctaccgcgg ggaatttcag catgggaatg ctggtgttta tcttcgtcgc tcgggtcatt
    11401 accaccttac tctgcttctc ttccggcgcg ccgggcggta tttttgcccc gatgctggcg
    11461 ctgggtactg tgctgggaac cgctttcgga atggttgccg ttgagctgtt tccgcaatat
    11521 cacctggagg cggggacgtt tgctattgcc ggaatggggg cattactggc ggcatctatt
    11581 cgcgcgccgt taacggggat cattctggtt ctggagatga ccgataacta ccagctcatt
    11641 ttgccaatga ttattaccgg tcttggcgca acactattag cgcaatttac cggcgggaaa
    11701 ccgctatact cggcgattct tgcgcgcacg ctggcaaaac aggaagctga gcaactggcg
    11761 cgaagcaagg ctgcatcagc cagcgagaat acttgaacga aataccaggg tattagataa
    11821 tggcgattat tattgggtta gaatttgccc aattgccgat gtcgtttgga gcaaaatatg
    11881 agtgatgacg tagcactgcc gctggagttt accgacgcag cagccaacaa agttaaaagc
    11941 ctgatcgctg acgaagataa cccgaatctg aaattacgcg tgtatatcac cggtggcggt
    12001 tgcagcggct tccagtatgg tttcaccttt gatgatcagg tgaacgaagg cgatatgacc
    12061 atcgaaaaac agggcgttgg cctggtggtt gatccgatga gcctgcaata tctggtcggc
    12121 ggttccgttg actacaccga aggactggaa ggttctcgtt tcatcgtgac caacccgaac
    12181 gcgaaaagca cctgcggttg cggttcttcc tttagcatct aatctgttgt tcatgtgccg
    12241 gataaactat ccggcacatc ttctctcttc aacgcccatt ctcatccagc gcaaacgtcg
    12301 gtagcttaag atgccaacga atagccgcca gccgaatcaa tagcgtcacg accatgccca
    12361 tcatactggc tgtttccagt ggtacggaaa atgtgtaata agccgtagcg tggacaatac
    12421 cgccgataat acaggcagtt gcgtagattt ctgtacgtaa aatcatgggg atctcgcggg
    12481 ccagtacatc acgaatgatc ccgccgccaa cgccagtaat gacgcccata caaaccgcga
    12541 ttaacggacc ggcttccgca ttaaaggctt tattcacgcc aatgccgaca aacaccgcca
    12601 gaccaacggc gtccagcacc ggcaacatcc attttggtaa gcgtcttggc tggcgcacca
    12661 gcacgatggt cagcatgctg gtgaccattg caacgaccag atcggtggga tctttcaccc
    12721 aaaataccgg gccgtgatcc agcgccatgt cgcgaattgt tccgccgcct actgcggtaa
    12781 ccacgcccag taccagaaca ccaaaagggt ccatacgcaa ttttccggct aacaaaacgc
    12841 cggagatggc aaataccgct gtgccgacta tatccagcca atagacgagc attgttcaat
    12901 ccccactgag cacctgaaaa ggtcaggcac taatctacct gtgaaagcgc attacagagc
    12961 tgttgtgcag cgaggataat acgtgggctt gcacgttcaa accagtcact cgtgagagga
    13021 ataacgggaa ttttgagctg ttcaccccag tattgtttga ttttaggaat ttggtccggt
    13081 ccgcctgtaa tgacaatcgc ctgtggcgag cgtgctaaca cctgttcgcg gctaacttgc
    13141 ggccagggaa cccggctgtc tttaaagatg ttttctccgc cacaaacttc gagtacctgg
    13201 ttctgaatcg actcttttcc actggtaaat ggcggattaa tgccgaattg cagaaaaaca
    13261 cgttttttag gtttatcagc atattgcgct ttcaattgcg cgtactgatc cagcagggat
    13321 tgcgcggctt gttcggcctt gtctggttgc ggactccagg gggccagttg acgtaacgca
    13381 ttggcaattt gttcaatgct tgtcgcatcg acccacatca cttttattcc cagcgaagcc
    13441 agctggtcaa cctgccgctc ggcattacct ccacgccagg caatcaccag atcgggcttc
    13501 agcgcgacaa tgcgttccag attcatcccc tgccaggtgg aaacctgctc aatcttttgc
    13561 gcttgtggag gatagtcgga atagctgctg accccaaccg gcgtgatccc ggcagcaaag
    13621 gcaagttcag tgttggcggg agaaagcgtg atgacgcgcg gcgcggcgtt gagccacagt
    13681 ggcgcaagaa aagacagggc gaccagcgcc ctgaacagtg acttagccat gtgcaagttt
    13741 ctgcaccagt gactcaacca tcaggctgga ctgtttagcg gcaacagcca ggaactcatc
    13801 gaagctaaga tgagactgtt gatcggccac gtcggagatg gcgcgtacga caacaaacgg
    13861 gacgttgaaa ttgtggcaga catgggcgat tgccgtcgct tccatctcta cagcaatggc
    13921 ctgtgggaag ttgtggcgga ttttcgccag accaacagaa ccgttgatga aagcgtcgcc
    13981 gctaacaatc aggccacgta cagcgttaag attcagttcg gcaatgcagg cctcagcggc
    14041 agcgatcagt ttatcgtcag ctttaaagcc tgccggacag cctggtaact gaccgtattc
    14101 ataaccaaat gccgtgacat ccgcgtcgtg ataacgtgct tcgtccgaga caacgatatc
    14161 gcccactttc aacgttggtg ccaggccacc ggcagaaccg gtgttaataa tcacatctgg
    14221 cttgcagtgt tccaacagca gagtggcacc cagcgccgca gcgactttac cgatgcccga
    14281 tttcagaagc gcaacctcgg ttccattcag ttggccggta tagatttcgc aaccgccgag
    14341 actgatagtt tgacggtttt cgattttgtc acgcagcagc gtaacttctt cttccattgc
    14401 accaatgatg ccgattttca tagatttact cgcgataagc ccgatttgaa ggcatagttt
    14461 accatgcgct tacggggaag cgtatttctc acgcgggaga ggacatggca cagattgatt
    14521 tccgaaaaaa aataaactgg catcgtcgtt accgttcacc gcagggcgtt aaaaccgaac
    14581 atgagatcct gcggatcttc gagagcgatc gcgggcgtat catcaactct ccggcaattc
    14641 gtcgtctgca acaaaagacc caggtttttc cactggagcg caatgccgcc gtgcgcacgc
    14701 gtcttaccca ctcgatggaa gtccagcagg tggggcgcta catcgccaaa gaaattttaa
    14761 gccgtctgaa agagcttaaa ttgctggaag catacggcct ggatgaactg accggacctt
    14821 ttgaaagcat tgttgagatg tcatgcctga tgcacgatat cggcaatccg ccgtttggtc
    14881 attttggcga agcggcgata aatgactggt ttcgccagcg tttatacccg gaagatgccg
    14941 aaagccagcc tctgactgac gatcgctgca gcgtggcggc actacgttta cgggacgggg
    15001 aagaaccgct taacgagctg cggcgcaaga ttcgtcagga cttatgtcat tttgagggga
    15061 atgcacaagg cattcgtctg gtgcatacat tgatgaggat gaatctcacc tgggcacagg
    15121 ttggcggtat tttaaaatat acccgtccgg cgtggtggcg tggcgaaacg cctgagacac
    15181 atcactattt aatgaaaaag ccgggttatt atctttctga agaagcctat attgcccggt
    15241 tgcgtaaaga acttaatttg gcgctttaca gtcgttttcc attaacgtgg attatggaag
    15301 ctgccgacga catctcctat tgtgtggcag accttgaaga tgcggtagag aaaagaatat
    15361 ttaccgttga gcagctttat catcatttgc acgaggcgtg gggtcagcat gagaaaggtt
    15421 cgctcttttc gctagtggtt gaaaatgcct gggaaaaatc acgctcaaat agtttaagcc
    15481 gcagtacgga agatcagttt tttatgtatt tacgggtaaa caccctaaat aaactggtac
    15541 catacgcggc acaacgattt attgataatc tgcctgcgat tttcgccgga acgtttaatc
    15601 atgcattatt ggaagatgcc agcgaatgca gcgatcttct taagctatat aaaaatgtcg
    15661 ctgtaaaaca tgtgtttagc catccggatg tcgagcagct tgaattgcag ggctatcggg
    15721 tcattagcgg attattagag atttatcgtc ctttattaag cctgtcgtta tcagacttta
    15781 ctgaactggt agaaaaagaa cgggtgaaac gtttccctat tgaatcgcgc ttattccaca
    15841 aactctcgac gcgccatcgg ctggcctatg tcgaggctgt cagtaaatta ccgtcagatt
    15901 ctcctgagtt tccgctatgg gaatattatt accgttgccg cctgctgcag gattatatca
    15961 gcggtatgac cgacctctat gcgtgggatg aataccgacg tctgatggcc gtagaacaat
    16021 aaccaggctt ttgtaaagac gaacaataaa tttttacctt ttgcagaaac tttagttcgg
    16081 aacttcgcgc tataaaacga atctgaagaa cacagcaatt ttgcgttatc tgttaatcga
    16141 gactgaaata catgaaaaaa accacattag cactgagtgc actggctctg agtttaggtt
    16201 tggcgttatc tccgctctct gcaacggcgg ctgagacttc ttcagcaacg acagcccagc
    16261 agatgccaag ccttgcaccg atgctcgaaa aggtgatgcc ttcagtggtc agcattaacg
    16321 tagaaggtag cacaaccgtt aatacgccgc gtatgccgcg taatttccag cagttcttcg
    16381 gtgatgattc tccgttctgc caggaaggtt caccgttcca gagctctccg ttctgccagg
    16441 ggggccaggg cggtaatggc ggcggccagc aacagaaatt catggcgctg ggttccggcg
    16501 tcatcattga tgccgataaa ggctatgtcg tcaccaacaa ccacgttgtt gataacgcga
    16561 cggtgattaa agtccagctg agcgatggtc gtaagttcga cgcgaagatg gttggcaaag
    16621 atccgcgctc tgatatcgcg ctgattcaga tccagaaccc gaaaaacctg accgcaatta
    16681 agatggcgga ttctgatgca ctgcgcgtgg gtgattacac cgtagcgatt ggtaacccgt
    16741 ttggcctggg agagacggta acttccggga ttgtctctgc gctggggcgt agcggcctga
    16801 atgccgaaaa ctacgaaaac ttcatccaga ccgatgcagc gatcaaccgt ggtaactccg
    16861 gtggtgcgct ggttaacctg aacggcgaac tgatcggtat caacaccgcg atcctcgcac
    16921 cggacggcgg caacatcggt atcggttttg ctatcccgag caacatggtg aaaaacctga
    16981 cctcgcagat ggtggaatac ggccaggtga aacgcggtga gctgggtatt atggggactg
    17041 agctgaattc cgaactggcg aaagcgatga aagttgacgc ccagcgcggt gctttcgtaa
    17101 gccaggttct gcctaattcc tccgctgcaa aagcgggcat taaagcgggt gatgtgatca
    17161 cctcactgaa cggtaagcca atcagcagct ttgccgcact gcgtgctcag gtgggtacta
    17221 tgccggtggg cagcaaactg accctgggct tgctgcgcga cggtaagcag gtcaacgtga
    17281 atctggaact gcagcagagc agccagaatc aggttgattc cagctccatc ttcaacggca
    17341 ttgaaggtgc tgagatgagc aacaaaggca aagatcaggg cgtggtagtg aacaacgtga
    17401 aaacgggcac tccggctgcg cagatcggcc tgaagaaagg tgatgtgatt attggcgcga
    17461 accagcaggc agtgaaaaac atcgctgaac tgcgtaaagt tctcgacagc aaaccgtctg
    17521 tgctggcact gaacattcag cgcggcgaca gctccatcta cctgttaatg cagtaatctc
    17581 cctcaacccc ttcctgaaaa cgggaagggg ttctccttac aatctgtgaa cttcaccaca
    17641 actccataca tcttcatcat cctttaggca tttgcacaat gccgtacgtt acgtacttcc
    17701 ttatgctaag ccgtgcataa cggaggactt atggctggct ggcatcttga taccaaaatg
    17761 gcgcaggata tcgtggcacg taccatgcgc atcatcgata ccaatatcaa cgtaatggat
    17821 gcccgtgggc gaattatcgg cagcggcgat cgtgagcgta ttggtgaatt gcacgaaggt
    17881 gcattgctgg tactttcaca gggacgagtc gtcgatatcg atgacgcggt ggcacgtcat
    17941 ctgcacggtg tgcggcaagg gattaatcta ccgttacggc tggaaggtga aattgtcggc
    18001 gtaattggcc tgacaggtga accagagaat ctgcgtaaat atggcgaact ggtctgcatg
    18061 acggctgaaa tgatgctgga acagtcgcgg ttgatgcact tgttggctca ggatagccgt
    18121 ttgcgggagg aactggtgat gaacctgatt caggcagagg agaatactcc cgcacttact
    18181 gaatgggcgc aacggctggg gatcgatctc aatcaaccgc gagtggtggc tattgttgag
    18241 gtcgacagcg gtcagcttgg tgtggacagc gcaatggcgg agttacaaca actgcaaaac
    18301 gcgctgacta cgcccgagcg taataatctg gtggcgattg tctcgctaac cgaaatggtg
    18361 gtgttgaaac cggcgttgaa ctcttttggg cgctgggatg cagaagatca tcgtaagcga
    18421 gttgaacaac tgattacccg catgaaagag tacggccagc tgcgttttcg cgtttcactg
    18481 ggcaactatt ttaccggtcc tggcagtatt gcccgatcct atcgtacggc gaaaacgacg
    18541 atggtggtgg gtaaacagcg gatgccagaa agtcgctgct atttttatca ggatctgatg
    18601 ttacctgtgt tactcgacag tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg
    18661 ctggcgcggc tgaaagcgat ggacaataac ggcttgctgc gacgaacgct ggcggcgtgg
    18721 tttcgtcaca atgtgcaacc gctggcaacg tcaaaggcgt tgtttattca tcgtaatact
    18781 ctggagtatc ggcttaatcg tatatcggaa ctgaccgggc ttgatttggg taattttgat
    18841 gacaggttgc tgctgtatgt ggcgttgcag ctggatgaag agcggtaggt tatgcgttaa
    18901 ggtcgaggcg gcgctggctc attcccctca gcccgaaggg tagaggggaa tgtccgagtt
    18961 aaccgtcagt tcggcattat ttacgcgtta gtttttccag atcggcttca atctcgctga
    19021 tcttattggt tacgactgac tccaggtgac gtaagtcgtc gaggatctta cgcttaagat
    19081 caacttcgct gcggtcacgc tggcagattt gatcaagctc atcaatgata taccgtagat
    19141 tcgggctgat ttcctggact tctttataac cctgacccac accatcagcg acgaccgttt
    19201 tacgctgacg cggatattta aacttaacgc tcttggcgaa aaactcgcct ttgtctttct
    19261 ggaaatagat tttcagaata tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt
    19321 cttcaggatt ggtaataccc agacttttca gattgtcgta catagcgtta cctcaaaatg
    19381 agtcagtaaa ttgtgcttat tttagcattt ggcctggccc gcccggctcg actgtgatcg
    19441 cagaaagcct ttttcttcca gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt
    19501 tattgtggcg atattccatg ggatggatta acacatttat cttcgttttg cgaattacat
    19561 gacagtccga aacaagatga aaaagtattt aaaaaagggg ggaagaagaa tgagtaggac
    19621 gaattattct gagccagaaa gcatacgggc agcatcatgc tgcccgtata tgtactctta
    19681 atcgatggta cgcagcagtt cgttaatgcc gactttgccg cgagttttcg cgtcaacttt
    19741 cttaacgata accgcacagt agaggctgta tttgccatct ttcgacggca gattacctga
    19801 aacaaccaca gaccccgccg gaacgcgacc gtagtggatt tcgccggttt cacggtcgta
    19861 aatacgggtg ctctgaccaa tgtatacgcc catggaaatg acggaacctt cttcgacaat
    19921 caccccttca accacttcag agcgtgcgcc aatgaagcag ttatcttcaa tgatggttgg
    19981 gttagcctgc agcggttcca gcacgccgcc gatgcccacg ccaccggaaa ggtggacgtt
    20041 tttaccaatc tgcgcacaag aaccgacggt cgcccaggta tcaaccatcg tgccttcatc
    20101 aacatatgcg ccgatgttga cgtaagacgg catcagcacg gtgttacggg caataaatgc
    20161 accctgacgt accgccgctg gtggcacaac gcggaagcct tctttctgaa aacgcgcttc
    20221 gtcgtagtcg gcgaatttca tcggcacttt atcgaagtag cggctttctg ccccttcgat
    20281 cacctgatta tcattaatac ggaaagagag cagcaccgct tttttcaacc actgatgcgt
    20341 cacccactga ccgtcaattt tttccgctac acgcagtgcg ccggaatcca gcagggcgat
    20401 cacctgattt accgcttcgc gggttacggt gtctgcattg gctggcgtga tctcggcacg
    20461 gcgttcaaaa gcggtttcaa taatgttctg taactgctgc attgttaaac tcttttcata
    20521 tcagtaaaca catcaccctt tatcgtttgg attgagggcc tctgtcaacc gctgatgcac
    20581 ttcctgctgc aactcgttat taagtgcacg ccggtcagcg gtggcaatta tgaataaatc
    20641 ttctactcgc tcgccaatgg ttgtaattcg ggcaccatga agcgaaattc ccagatcggc
    20701 aaaaattttc ccgactctcg ccagcagtcc tggctggtcg agcgcgataa gctcaaggaa
    20761 cgatttgcgg tcggtatggg tcggcaaaaa cgttacttcg gtttcaacag taaaatggcg
    20821 taatttggcg ggttggcgac ggggctgcgg tggctgccag ctactttgcg tcagtacttg
    20881 ctccagacca aaccgaataa cctcatgacg atctgcggac agcgggctgc catcgggttc
    20941 cagcacgata aaggtatcca tcgccatacc gtcgcgagtg gtgaaaattt gtgcgtcgtg
    21001 aacacttaaa ttgcggcggt ctaattcggc acagacggcg gcaaacagat aggggcggtc
    21061 cgggctccag ataaaaatct cggtgcctcc acgcgtagcc tgcgggctaa gcaataccag
    21121 cggtttgctt aaatcatgct gtaataaatg gcgggcatgc caggccagtt gatttgggct
    21181 atggcggaca aaatagttag cacgacagcg tgaccaaatt tggtgcagcg cctcttcgtc
    21241 gatgttatcc atgcgcagta gtgccagtgc ctggagttgg tgatggcgaa cccgttcgcg
    21301 catatccggc gtgttttgca ttccgcgtcg tagctgcttt tcggtggcaa aatagagttc
    21361 gcgcaacaga ctttgcttcc agctattcca cagcgtttcg ttggtggcgc aaatgtcagc
    21421 cactgtcagg cataccagat agcgcagacg attttccgtt tgaacttctt cggcaaactg
    21481 cttgatgact tcagggtcct gaatatcgcg gcgttgtgct gtcaccgaca tcagcaagtg
    21541 ctggcgaacc agccaggcaa ccagctgtgt ttcgcgtgag ttcagcccgt ggagttctgc
    21601 aaaatgcact acatcctgag caccgagaat ggagtggtcg ccgccgcgtc ctttggcgat
    21661 atcgtgaaac agcgcggcga tgaaaatcag ctcagttgac ggcaggcgcg gccagacgtc
    21721 cacacacaac ggatggcgct ggcgcgtttc ttcactggca aaactctcca gtttcagcat
    21781 cacgcggata gtatgttcat ccaccgtgta ggcgtggaac agatcaaact gcatctgccc
    21841 gacgatatgc gaccattgcg gcatatacgc gccgagcacg ctatggcgat gcattggcaa
    21901 tagcccgcgc cgcaccgctc cggggtgacg cagaatgctc aaaaacagtt ttcgtgcttc
    21961 cggaatatta cacagcggtt gttgcagatg gcgacgggca tggcgtaact ggcgcagcgt
    22021 ggtggagtaa atgccggtga tcgcactgtt gcgcaccatg gtgtagaaca tacgcaagat
    22081 ggcttccggc tggcgcataa atagtgtttc atcacgcagg tcgattagcg taccgcgtag
    22141 ctgaaactca tcgtcgattg gacgtggttt ttcgtcggcg ggaagggcga ggatggcttc
    22201 atcgaacagt tgcagcagca tctggttgag ttcactgacg cggcgtgtaa cgcggaagta
    22261 atccttcatc atccgctcga ccggttcgtt gccttcacca ctgtaattca gacgctgagc
    22321 gacgctaagt tggcgatcga ataacagacg attatcgtaa cggctgacga ccagatgcag
    22381 ggcaaagcga atacgccaca gtatatgcag acattcgttt aattccgccc gctccgctga
    22441 ggttaagaag ccaaacccga ccatttcatc cagcgatgtt gcgccaaaat gacggcgggc
    22501 cacccattgc agagtgtgaa tatcgcgcaa gccgccaggg ctacttttga tgtctggttc
    22561 aaggttgtag ctggtgccat ggtaacgctg atggcgctgg ttctgttctt caactttcgc
    22621 cgcgtagaac ttgtcggaag gccagaatcc ttcgctaaaa atatgttttt gcagttcgag
    22681 gaagagcgca acatcgccaa ttaataagcg ggattcgatt aaattggtgg cgacggttaa
    22741 atccgataac ccttccagca tgcactcttc aagcgtgcgc acactatgac cgacttccag
    22801 ctttacatcc cagagcagcg ttaacagctc gcccactttt tgcgcctgat cgtccgggag
    22861 ctttttacgg cttaaaatca gtaaatcgat gtcagaaagt ggatgcagct cgccacgacc
    22921 gtagccaccg acggcgacca atgccaggtc ggcaatctgg ctgaatcccg cttcaatcca
    22981 taatcgttgc aggagctggt cgataaactc ggtgcgcgcc tcaatcaact gttctgcaga
    23041 gatcccattg tcaaaggcat cacccagcca acgctggaaa gtatcgatat gggcttttat
    23101 cccaccgacg gttaattcat cacggggcca gacgcatgga ttttgcggtt gaccgggcag
    23161 ggtggggaga gcggtgtttg cgtactgttc tggaagggta ttcattgtgc gccacccata
    23221 agattaaatt atcgcattaa aaaagccggc attcgccggc ttcatcttat tcgtcgtgcg
    23281 agattatcgc cgggatggtg tcatccttgc gtagcgtcaa aatttcgcag ccgttatcag
    23341 tcaccacaat agtatgctca tattgtgcag acaagctgcg atctttggtt tttaccgtcc
    23401 agccatcttt catggtgcgg atctcttttt tacccgcgtt gaccattggc tcaatggtga
    23461 acgtcatccc cggtttcagc acgacgttgg tttcacggga gtcatagtgc agcacctgcg
    23521 gttcttcatg gaagccgcga ccaataccgt gtccacaata ttcacgaacg acggagaagc
    23581 cttctgcttc gacaaacttc tgaatcgccg caccgatttc gcgcaggtta atgcctggtt
    23641 tgaccatgcg cagcgccagg tacaggcttt cttgcgtgat gcggcacaga cgttcgccca
    23701 tgatggtcgg cttaccgacg ataaacattt tcgaggtatc gccgtggaaa ccatctttga
    23761 ttacggtgac atcaatgtta acgatatcgc catctttcag cagcttagca tcgtccggga
    23821 taccgtggca caccacttca ttaatagaga tgcaaacgga tttcggatag ccgtgatagc
    23881 cgaggcaggc agaaaccgcg tgttgttcat taacaatgta atcattacag atgcgatcca
    23941 gctcgccggt gctgacgccc ggtttaacat acggttcgat catctccagc acttcggcag
    24001 ccagtcggcc agcgacgcgc attttttcga tatcttctgg ggtcttgatt gagatagcca
    24061 ttaattctgt ccatcagcgt cggtgatacc gacaatatat atgtaagtgc cgtcaatggt
    24121 atcacacccg ggcaaattga gaatcattct gaatttcgcc aaacgtgcca ctgaaggttt
    24181 tctataatag aaaattcgac atctgatgct gtacacagcg ccaacaatta ttggtgtcca
    24241 tgacgtattt gtggtataaa gcgcgccgga cttccgatcc atttcgtata cacagactgg
    24301 acggaagcga caatctcact ttgtgtaaca acacacacgt atcggcacat attccggggt
    24361 gccctttggg gtcggtaata tgggatacgt ggaggcataa ccccaacttt tatatagagg
    24421 ttttaatcat ggcaactgtt tccatgcgcg acatgctcaa ggctggtgtt cacttcggtc
    24481 accagacccg ttactggaac ccgaaaatga agccgttcat cttcggtgcg cgtaacaaag
    24541 ttcacatcat caaccttgag aaaactgtac cgatgttcaa cgaagctctg gctgaactga
    24601 acaagattgc ttctcgcaaa ggtaaaatcc ttttcgttgg tactaaacgc gctgcaagcg
    24661 aagcggtgaa agacgctgct ctgagctgcg accagttctt cgtgaaccat cgctggctgg
    24721 gcggtatgct gactaactgg aaaaccgttc gtcagtccat caaacgtctg aaagacctgg
    24781 aaactcagtc tcaggacggt actttcgaaa agctgaccaa gaaagaagcg ctgatgcgca
    24841 ctcgtgagct ggagaaactg gaaaacagcc tgggcggtat caaagacatg ggcggtctgc
    24901 cggacgctct gtttgtaatc gatgctgacc acgaacacat tgctatcaaa gaagcaaaca
    24961 acctgggtat tccggtattt gctatcgttg ataccaactc tgatccggac ggtgttgact
    25021 tcgttatccc gggtaacgac gacgcaatcc gtgctgtgac cctgtacctg ggcgctgttg
    25081 ctgcaaccgt acgtgaaggc cgttctcagg atctggcttc ccaggcggaa gaaagcttcg
    25141 tagaagctga gtaataaggc ttgataactc ccccaaaata gttcgagttg cagaaaggcg
    25201 gcaagctcga gaattcccgg gagcttacat cagtaagtga ccgggatgag cgagcgaaga
    25261 taacgcatct gcggcgcgaa atatgaaggg ggagagccct tatagaccag gtagtacacg
    25321 tttggttagg gggcctgcat atggccccct ttttcacttt tatatctgtg cggtttaatg
    25381 ccgggcagat cacatctccg aggattttag aatggctgaa attaccgcat ccctggtaaa
    25441 agagctgcgt gagcgtactg gcgcaggcat gatggattgc aaaaaagcac tgactgaagc
    25501 taacggcgac atcgagctgg caatcgaaaa catgcgtaag tccggtgcta ttaaagcagc
    25561 gaaaaaagca ggcaacgttg ctgctgacgg cgtgatcaaa accaaaatcg acggcaacta
    25621 cggcatcatt ctggaagtta actgccagac tgacttcgtt gcaaaagacg ctggtttcca
    25681 ggcgttcgca gacaaagttc tggacgcagc tgttgctggc aaaatcactg acgttgaagt
    25741 tctgaaagca cagttcgaag aagaacgtgt tgcgctggta gcgaaaattg gtgaaaacat
    25801 caacattcgc cgcgttgctg cgctggaagg cgacgttctg ggttcttatc agcacggtgc
    25861 gcgtatcggc gttctggttg ctgctaaagg cgctgacgaa gagctggtta aacacatcgc
    25921 tatgcacgtt gctgcaagca agccagaatt catcaaacca gaagacgtat ccgctgaagt
    25981 ggtagaaaaa gaataccagg tacagctgga tatcgcgatg cagtctggta agccgaaaga
    26041 aatcgcagag aaaatggttg aaggccgcat gaagaaattc accggcgaag tttctctgac
    26101 cggtcagccg ttcgttatgg aaccaagcaa aactgttggt cagctgctga aagagcataa
    26161 cgctgaagtg actggcttca tccgcttcga agtgggtgaa ggcatcgaga aagttgagac
    26221 tgactttgca gcagaagttg ctgcgatgtc caagcagtct taattatcaa aaaggagccg
    26281 cctgagggcg gcttcttttt gtgcccatct tgtaaattca gctaaccctt gtggggctgc
    26341 gctgaaaagc gacgtacaat gtcgctagta ttaattcatt tcaatcgttg acagtctcag
    26401 gaaagaaaca tggctaccaa tgcaaaaccc gtctataaac gcattctgct taagttgagt
    26461 ggcgaagctc tgcagggcac tgaaggcttc ggtattgatg caagcatact ggatcgtatg
    26521 gctcaggaaa tcaaagaact ggttgaactg ggtattcagg ttggtgtggt gattggtggg
    26581 ggtaacctgt tccgtggcgc tggtctggcg aaagcgggta tgaaccgcgt tgtgggcgac
    26641 cacatgggga tgctggcgac cgtaatgaac ggcctggcaa tgcgtgatgc actgcaccgc
    26701 gcctatgtga acgctcgtct gatgtccgct attccattga atggcgtgtg cgacagctac
    26761 agctgggcag aagctatcag cctgttgcgc aacaaccgtg tggtgatcct ctccgccggt
    26821 acaggtaacc cgttctttac caccgactca gcagcttgcc tgcgtggtat cgaaattgaa
    26881 gccgatgtgg tgctgaaagc aaccaaagtt gacggcgtgt ttactgctga tccggcgaaa
    26941 gatccaactg caaccatgta cgagcaactg acttacagcg aagtgctgga aaaagagctg
    27001 aaagtcatgg acctggcggc cttcacgctg gctcgtgacc ataaattacc gattcgtgtt
    27061 ttcaacatga acaaaccggg tgcgctgcgc cgtgtggtaa tgggtgaaaa agaagggact
    27121 ttaatcacgg aataattccc gtgatggata aataagggta agattccgca taagtatcgc
    27181 gggggcgtaa gtctggttat aaggcgttat tgttgcaggc agtttggtca cggccagcgc
    27241 gcagcaaccg gtgcgtacac aagtacgtga ggatggcgag cactacccgg ggccaaaatg
    27301 gcaaataaaa tagcctaata atccagacga ttacccgtaa tatgtttaat cagggctata
    27361 cttagcacac ttccactgtg tgtgactgtc tggtctgact gagacaagtt ttcaaggatt
    27421 cgtaacgtga ttagcgatat cagaaaagat gctgaagtac gcatggacaa atgcgtagaa
    27481 gcgttcaaaa cccaaatcag caaaatacgc acgggtcgtg cttctcccag cctgctggat
    27541 ggcattgtcg tggaatatta cggcacgccg acgccgctgc gtcagctggc aagcgtaacg
    27601 gtagaagatt cccgtactct gaaaatcaac gtgtttgatc gttcaatgtc tccggccgtt
    27661 gaaaaagcga ttatggcgtc cgatcttggc ctgaacccga actctgcggg tagcgacatc
    27721 cgtgttccgc tgccgccgct gacggaagaa cgtcgtaaag atctgaccaa aatcgttcgt
    27781 ggtgaagcag aacaagcgcg tgttgcagta cgtaacgtgc gtcgtgacgc gaacgacaaa
    27841 gtgaaagcac tgttgaaaga taaagagatc agcgaagacg acgatcgccg ttctcaggac
    27901 gatgtacaga aactgactga tgctgcaatc aagaaaattg aagcggcgct ggcagacaaa
    27961 gaagcagaac tgatgcagtt ctgatttctt gaacgacaaa aacgccgctc agtagatcct
    28021 tgcggatcgg ctggcggcgt tttgcttttt attctgtctc aactctggat gtttcatgaa
    28081 gcaactcacc attctgggct cgaccggctc gattggttgc agcacgctgg acgtggtgcg
    28141 ccataatccc gaacacttcc gcgtagttgc gctggtggca ggcaaaaatg tcactcgcat
    28201 ggtagaacag tgcctggaat tctctccccg ctatgccgta atggacgatg aagcgagtgc
    28261 gaaacttctt aaaacgatgc tacagcaaca gggtagccgc accgaagtct taagtgggca
    28321 acaagccgct tgcgatatgg cagcgcttga ggatgttgat caggtgatgg cagccattgt
    28381 tggcgctgct gggctgttac ctacgcttgc tgcgatccgc gcgggtaaaa ccattttgct
    28441 ggccaataaa gaatcactgg ttacctgcgg acgtctgttt atggacgccg taaagcagag
    28501 caaagcgcaa ttgttaccgg tcgatagcga acataacgcc atttttcaga gtttaccgca
    28561 acctatccag cataatctgg gatacgctga ccttgagcaa aatggcgtgg tgtccatttt
    28621 acttaccggg tctggtggcc ctttccgtga gacgccattg cgcgatttgg caacaatgac
    28681 gccggatcaa gcctgccgtc atccgaactg gtcgatgggg cgtaaaattt ctgtcgattc
    28741 ggctaccatg atgaacaaag gtctggaata cattgaagcg cgttggctgt ttaacgccag
    28801 cgccagccag atggaagtgc tgattcaccc gcagtcagtg attcactcaa tggtgcgcta
    28861 tcaggacggc agtgttctgg cgcagctggg ggaaccggat atgcgtacgc caattgccca
    28921 caccatggca tggccgaatc gcgtgaactc tggcgtgaag ccgctcgatt tttgcaaact
    28981 aagtgcgttg acatttgccg caccggatta tgatcgttat ccatgcctga aactggcgat
    29041 ggaggcgttc gaacaaggtc aggcagcgac gacagcattg aatgccgcaa acgaaatcac
    29101 cgttgctgct tttcttgcgc aacaaatccg ctttacggat atcgctgcgt tgaatttatc
    29161 cgtactggaa aaaatggata tgcgcgaacc acaatgtgtg gacgatgtgt tatctgttga
    29221 tgcgaacgcg cgtgaagtcg ccagaaaaga ggtgatgcgt ctcgcaagct gaggataatc
    29281 cggctacaga gagtcgcgct atttgttagc gtagggcttc agtgatatag tctgcgccat
    29341 ctgatcgtaa gtagttggct ttataaggtc aggtatgccg tggttttaca cggctttttt
    29401 ttgtataggc ttcagtattc ctgagtaccg taaaccctgt cagggaataa aaaacgcgtg
    29461 atgttgtctg ctactcaacc acttagcgaa aaattgccag cgcatggctg ccgtcatgtt
    29521 gcgatcatta tggacggcaa tggtcgctgg gcaaaaaagc aagggaagat tcgtgccttt
    29581 gggcataaag ccggggcaaa atccgtccgc cgggctgtct cttttgcggc caacaacggt
    29641 attgaggcgt taacgctgta tgcctttagt agtgaaaact ggaaccgacc agcgcaggaa
    29701 gtcagtgcgt taatggaact gtttgtgtgg gcgctcgata gcgaagtaaa aagtctgcac
    29761 cgacataacg tgcgtctgcg tattattggc gataccagtc gctttaactc gcgtttgcaa
    29821 gaacgtattc gtaaatctga agcgctaaca gccgggaata ccggtctgac gctgaatatt
    29881 gcggcgaact acggtggacg ttgggatata gtccagggag tcaggcaact ggctgaaaag
    29941 gtgcagcaag gaaacctgca accagatcag atagatgaag agatgctaaa ccagcatgtc
    30001 tgtatgcatg aactggcccc tgtagattta gtaattagga ctggggggga gcatcgcatt
    30061 agtaactttt tgctttggca aattgcctat gccgaacttt actttacaga tgttctctgg
    30121 cccgatttcg atgaacaaga ctttgaaggg gcgttaaatg cctttgctaa tcgagagcgt
    30181 cgtttcggcg gcaccgagcc cggtgatgaa acagcctgat gggggtcgct tttgctgaag
    30241 tatcgcctga tatctgcttt tgtgttaata cccgtcgtca tcgcggcgtt gtttctgttg
    30301 ccgccggtgg ggttcgccat cgtaacgctg gtggtctgca tgctggcagc gtgggaatgg
    30361 ggacagctta gcggttttac cactcgttcg cagcgagtat ggttggcggt gttatgcggg
    30421 ttattgttgg cgctgatgct ttttctgttg ccggaatatc accgaaatat tcatcaaccg
    30481 ctggttgaaa tctcactttg ggcttcgctg ggttggtgga ttgtcgcgct attgctggtg
    30541 ctgttttacc caggttccgc agcaatctgg cgtaactcta aaacattgcg ccttattttt
    30601 ggcgtgctaa ccattgttcc cttcttctgg ggcatgctgg cgttacgggc ctggcactat
    30661 gacgagaatc attacagtgg cgcaatatgg ctgctctatg tcatgatcct ggtatggggc
    30721 gctgactccg gcgcatatat gtttggcaaa ttgtttggta aacataagct ggcaccgaag
    30781 gtttctccgg gtaaaacctg gcaaggcttt ataggtggac tagcgaccgc agcggtaatc
    30841 tcatggggtt atggcatgtg ggcgaatctc gacgtcgctc ccgtcacctt actcatttgc
    30901 tctattgtcg cagcgttagc ctcagtgctc ggcgatctga ccgagagtat gtttaagcgt
    30961 gaagcaggaa ttaaggacag cggtcattta attccaggac acggtggtat tttagatcgt
    31021 attgatagcc tgacggctgc ggtaccggtc tttgcttgct tgttgttact ggtattcagg
    31081 acgctttaac ggaaggtaat atgctgagtt ttctctggga tttggcttcg ttcatcgttg
    31141 cactgggtgt acttatcacc gtgcatgaat ttggtcattt ctgggttgcc cggcgttgtg
    31201 gtgttcgcgt tgagcgtttc tcaatagggt ttggtaaggc gctctggcgg cgaactgata
    31261 agctcggcac cgaatatgtt atcgccctga tcccgctggg cggttatgtc aaaatgctgg
    31321 atgagcgcgc agaaccggtc gttccggaac tccgccacca tgccttcaat aataaatctg
    31381 ttggccaacg agcggcgatt attgccgcag gtccggttgc aaacttcatt tttgctatct
    31441 tagcctactg gctggttttt attattggtg tgcctggcgt acgtccggtg gttggtgaaa
    31501 tagcagccaa ttcgatagct gcggaagcac aaattgcacc aggtacggaa ctaaaagccg
    31561 tagatggtat cgaaacgcct gattgggatg ccgtgcgttt gcagttggtc gataaaattg
    31621 gcgatgaaag caccaccatt acagtagcgc catttggcag cgaccaacgg cgggatgtaa
    31681 agctcgattt acgtcactgg gcgtttgagc ctgataaaga agatccggta tcttcgctgg
    31741 ggattcgtcc tcgtgggccg caaattgaac ctgtactgga aaatgtgcag ccaaactcgg
    31801 cggcaagcaa ggcaggtttg caagcaggcg acaggatcgt taaagtcgat ggtcagccct
    31861 taacgcagtg ggtgaccttt gtgatgcttg tccgggataa cccgggtaaa tccttagcgt
    31921 tagaaatcga aaggcagggg agtcccttgt ctttgacatt aatcccggag agtaaaccgg
    31981 gtaatggtaa agcgattggt tttgtcggta ttgagccgaa agtcattcct ttgccagatg
    32041 agtataaagt tgtacgccag tatgggccgt tcaacgccat cgtcgaagcc acggacaaaa
    32101 cgtggcagct gatgaagctg acggtcagta tgctgggaaa attgatcacc ggtgatgtga
    32161 aactgaacaa cctcagtggg ccgatctcta tcgccaaggg ggctgggatg acagcggaac
    32221 tcggggtagt ttattacctg ccgtttcttg cgcttattag cgtgaactta gggataatta
    32281 acctgtttcc gttgcccgta cttgacgggg ggcatctgct gttccttgcg atcgaaaaga
    32341 tcaagggcgg accggtatcc gagcgggttc aagacttttg ttatcgcatt ggctcgattc
    32401 tgctggtgct gttaatgggg cttgcacttt tcaatgattt ctctcggtta tgagagttag
    32461 ttaggaagaa cgcataataa cgatggcgat gaaaaagttg ctcatagcgt cgctgctgtt
    32521 tagcagcgcc accgtatacg gtgctgaagg gttcgtagtg aaagatattc atttcgaagg
    32581 ccttcagcgt gtcgccgttg gtgcggccct cctcagtatg ccggtgcgca caggcgacac
    32641 ggttaatgat gaagatatca gtaataccat tcgcgctctg tttgctaccg gcaactttga
    32701 ggatgttcgc gtccttcgtg atggtgatac ccttctggtt caggtaaaag aacgtccgac
    32761 cattgccagc attactttct ccggtaacaa atcggtgaaa gatgacatgc tgaagcaaaa
    32821 cctcgaggct tctggtgtgc gtgtgggcga atccctcgat cgcaccacca ttgccgatat
    32881 cgagaaaggt ctggaagact tctactacag cgtcggtaaa tatagcgcca gcgttaaagc
    32941 tgtcgtgacc ccgctgccgc gcaaccgtgt tgacctaaaa ctggtgttcc aggaaggtgt
    33001 gtcagctgaa atccagcaaa ttaacattgt tggtaaccat gctttcacca ccgacgaact
    33061 gatctctcat ttccaactgc gtgacgaagt gccgtggtgg aacgtggtag gcgatcgtaa
    33121 ataccagaaa cagaaactgg cgggcgacct tgaaaccctg cgcagctact atctggatcg
    33181 cggttatgcc cgtttcaaca tcgactctac ccaggtcagt ctgacgccag ataaaaaagg
    33241 tatttacgtc acggtgaaca tcaccgaagg cgatcagtac aagctttctg gcgttgaagt
    33301 gagcggcaac cttgccgggc actccgctga aattgagcag ctgactaaga tcgagccggg
    33361 tgagctgtat aacggcacca aagtgaccaa gatggaagat gacatcaaaa agcttctcgg
    33421 tcgctatggt tatgcctatc cgcgcgtaca gtcgatgcct gaaattaacg atgccgacaa
    33481 aaccgttaaa ttacgcgtga acgttgatgc gggtaaccgt ttctacgtgc gtaagatccg
    33541 ttttgaaggt aacgatacct cgaaagatgc cgtcctgcgt cgcgaaatgc gtcagatgga
    33601 aggtgcatgg ctgggaagcg atctggtcga tcagggtaag gagcgtctga atcgtctggg
    33661 cttctttgaa actgtcgata ccgataccca acgtgttccg ggtagcccgg accaggtaga
    33721 tgtcgtctac aaggtaaaag agcgcaacac cggtagcttc aacttcggga ttggttacgg
    33781 tactgaaagt ggcgtgagct tccaggctgg cgtgcaacag gataactggt taggtacagg
    33841 ttatgctgtt ggtatcaacg ggaccaaaaa cgattaccag acctatgctg aactgtcggt
    33901 aaccaacccg tacttcaccg tagatggcgt aagcctcggt ggtcgtctct tctataatga
    33961 cttccaggca gatgacgccg acctgtccga ctataccaac aagagttatg gtacagacgt
    34021 gacgttgggc ttcccgatca acgaatataa ctcgctgcgt gcaggtctgg gttatgtaca
    34081 taactccctg tccaacatgc agccgcaggt tgcgatgtgg cgttatctgt actctatggg
    34141 tgaacatccg agcacctctg atcaggataa tagcttcaaa acggacgact tcacgttcaa
    34201 ctatggttgg acctataaca agcttgaccg tggttacttc ccgacagatg gttcacgtgt
    34261 caacctgacc ggtaaagtga ccattcctgg atcggataac gaatactaca aagtgacgtt
    34321 agacacggcg acttatgtgc cgatcgatga cgatcacaaa tgggttgttc tggggcgtac
    34381 ccgctggggt tatggtgatg gtttaggcgg caaagagatg ccgttctacg agaacttcta
    34441 tgccggtggt tccagcaccg tgcgtggctt ccagtccaat acaattggtc cgaaagcagt
    34501 ttacttcccg catcaggcca gtaattatga tccggactat gattacgaat gtgcgactca
    34561 ggacggcgcg aaagacctgt gtaaatcgga tgatgctgta ggcggtaacg ccatggcgat
    34621 tgccagcctc gagttcatca ccccgacgcc gtttattagc gataagtatg ctaactcggt
    34681 tcgtacttcc ttcttctggg atatgggtac cgtttgggat acaaactggg attccagcca
    34741 atattctgga tatccggact atagtgatcc aagcaatatc cgtatgtctg cgggtatcgc
    34801 attacaatgg atgtccccat tggggccgtt ggtgttctcc tacgcccagc cgttcaaaaa
    34861 gtacgatgga gacaaggcag aacagttcca gtttaacatc ggtaaaacct ggtaagtgtt
    34921 ctccacaaag gaatgtagtg gtagtgtagc gatgacttta ggcgatcaat ataagatcgc
    34981 cgggccacgc aaagaactgc accctccggt gcaaatggga tggtaaggag tttattgtga
    35041 aaaagtggtt attagctgca ggtctcggtt tagcactggc aacttctgct caggcggctg
    35101 acaaaattgc aatcgtcaac atgggcagcc tgttccagca ggtagcgcag aaaaccggtg
    35161 tttctaacac gctggaaaat gagttcaaag gccgtgccag cgaactgcag cgtatggaaa
    35221 ccgatctgca ggctaaaatg aaaaagctgc agtccatgaa agcgggcagc gatcgcacta
    35281 agctggaaaa agacgtgatg gctcagcgcc agacttttgc tcagaaagcg caggcttttg
    35341 agcaggatcg cgcacgtcgt tccaacgaag aacgcggcaa actggttact cgtatccaga
    35401 ctgctgtgaa atccgttgcc aacagccagg atatcgatct ggttgttgat gcaaacgccg
    35461 ttgcttacaa cagcagcgat gtaaaagaca tcactgccga cgtactgaaa caggttaaat
    35521 aagtaatgcc ttcaattcga ctggctgatt tagcgcagca gttggatgca gaactacacg
    35581 gtgatggcga tatcgtcatc accggcgttg cgtccatgca atctgcacaa acaggtcaca
    35641 ttacgttcat ggttaaccca aaataccgtg agcatttagg cttgtgccag gcgtccgcgg
    35701 ttgtcatgac ccaggacgat cttcctttcg cgaaaagtgc cgcgctggta gtgaagaatc
    35761 cctacctgac ttacgcgcgc atggcgcaaa ttttagatac cacgccgcag cccgcgcaga
    35821 acattgcacc cagtgcggtg atcgacgcga cggcgaagct gggtaacaac gtatcgattg
    35881 gcgctaacgc ggtgattgag tccggcgttg aactgggcga taacgtgatt atcggtgccg
    35941 gttgcttcgt aggtaaaaac agcaaaatcg gtgcaggttc gcgtctctgg gcgaacgtaa
    36001 ccatttacca tgagatccag atcggtcaga attgcctgat ccagtccgga acagtggtag
    36061 gcgcagacgg ctttggttat gccaacgatc gtggtaactg ggtgaagatc ccacagattg
    36121 gtcgcgtaat tattggcgat cgcgtggaga tcggtgcctg cacaaccatc gatcgcggcg
    36181 cgctggatga cactattatt ggcaatggcg tgatcattga taaccagtgc cagattgcac
    36241 ataacgtcgt gattggcgac aatacggcgg ttgccggtgg cgtcattatg gcgggcagcc
    36301 tgaaaattgg tcgttactgc atgatcggcg gagccagcgt aatcaacggg catatggaaa
    36361 tatgcgacaa agtgacggtt acgggcatgg gtatggtgat gcgtcccatc actgaaccag
    36421 gcgtctattc ctcaggcatt ccgctgcaac ccaacaaagt ctggcgcaaa accgctgcac
    36481 tggtgatgaa cattgatgac atgagcaagc gtctgaaatc gcttgagcgc aaggttaatc
    36541 aacaagacta acgttccatc ttttgttcgc caaactttac ggcctgtctc attcttacga
    36601 ttgcggcagg ccgtgttatt attgtcgttt cttatatttt gacaggaaga gtatcttgac
    36661 tactaacact catactctgc agattgaaga gattttagaa cttctgccgc accgtttccc
    36721 tttcttactg gtggatcgcg tgctggattt tgaagaaggt cgttttctgc gcgcagtaaa
    36781 aaatgtctct gtcaatgagc cattcttcca gggccatttc cctggaaaac cgattttccc
    36841 gggtgtgctg attctggaag caatggcaca ggcaacaggt attctggcgt ttaaaagcgt
    36901 aggaaaactg gaaccgggtg agctgtacta cttcgctggt attgacgaag cgcgcttcaa
    36961 gcgcccggtc gtgcctggcg atcaaatgat catggaagtc actttcgaaa aaacgcgccg
    37021 cggcctgacc cgttttaaag gggttgctct ggtcgatggt aaagtagttt gcgaagcaac
    37081 gatgatgtgt gctcgtagcc gggaggcctg atacgtgatt gataaatccg cctttgtgca
    37141 tccaaccgcc attgtggaag agggcgcgtc aattggcgcg aacgcacaca ttggtccttt
    37201 ttgtatcgtt ggaccccatg tcgaaattgg tgagggtacc gtactgaaat ctcacgttgt
    37261 cgtgaatggt catactaaaa ttggccgcga taatgagatt tatcagttcg cctccatcgg
    37321 cgaagttaac caggatctga aatatgctgg cgaaccgacc cgtgtggaaa tcggcgatcg
    37381 taaccgcatt cgcgaaagcg tcaccattca tcgtggcaca gtccagggcg gtggattgac
    37441 gaaggtgggc agcgacaact tactgatgat caacgcgcac attgcgcacg attgtacggt
    37501 aggtaaccgc tgtattctcg ccaacaacgc aacgctggcg ggtcacgtat cggttgacga
    37561 cttcgcgatc atcggcggca tgaccgcagt ccatcagttc tgcatcattg gtgcgcacgt
    37621 gatggttggc ggctgctccg gtgtggcgca ggacgtccct ccttatatca ttgcgcaggg
    37681 taaccacgca acgccgttcg gtgtcaatat cgaagggctg aagcgccgcg gattcagccg
    37741 tgaggcgatt accgctatcc gcaatgcgta taagctgatt tatcgtagcg gtaaaacgct
    37801 cgatgaagtg aaaccggaaa ttgctgaact ggcggaaaca tatccggaag tgaaagcctt
    37861 taccgatttc tttgcacgct caacgcgcgg tctgattcgt taatgactga acagcgtcca
    37921 ttaacgattg ccctggtcgc cggagaaacc tccggcgata tcctgggggc cggtttaatc
    37981 cgcgctctga aagaacgtgt gcccaacgcc cgctttgttg gtgttgccgg gccacgaatg
    38041 caggctgaag gctgcgaagc ctggtacgaa atggaagaac tggcggtgat gggcattgtt
    38101 gaagtgctcg gtcgtctgcg tcgcttactg catattcgtg ccgatctgac aaagcgtttt
    38161 ggcgaactga agccagatat ttttgttggt attgatgcgc ctgacttcaa tattactctt
    38221 gaaggtaacc tcaaaaagca gggtatcaaa accattcatt acgtcagtcc atccgtctgg
    38281 gcgtggcgac agaaacgcgt tttcaaaata ggcagagcca ccgatctggt gctcgcattt
    38341 ctgcctttcg aaaaagcgtt ttatgacaaa tacaatgtac cgtgccgctt tatcggtcat
    38401 accatggctg atgccatgcc gttagatcca gataaaaatg ccgcccgtga tgtgctgggg
    38461 atccctcacg atgcccactg cctggcgttg ctaccaggga gccgtggtgc agaagttgaa
    38521 atgcttagtg ccgatttcct gaaaacggcc cagcttttgc gccagacata tccggatctc
    38581 gaaatcgtgg tgccactggt gaatgccaaa cgccgcgagc agtttgaacg catcaaagct
    38641 gaagtcgcgc cagacctttc agttcatttg ctggatggga tgggccgtga ggcgatggtc
    38701 gccagcgatg cggcgctact ggcgtcgggt acggcagccc tggagtgtat gctggcgaaa
    38761 tgcccgatgg tggtgggata tcgcatgaag ccttttacct tctggttggc gaagcggctg
    38821 gtgaaaactg attatgtctc gctgccaaat ctgctggcgg gtagagagtt agtcaaagaa
    38881 ttattgcagg aagagtgtga gccgcaaaaa ctggctgcgg cgctgttacc gctgttggcg
    38941 aacgggaaaa ccagccacgc gatgcacgat accttccgtg aactgcatca gcagatccgc
    39001 tgcaatgccg atgagcaggc ggcacaagcc gttctggagt tagcacaatg atcgaatttg
    39061 tttatccgca cacgcagctg gttgcgggtg tggatgaagt cggacgcggg ccgttagttg
    39121 gcgcggtcgt caccgctgcg gtgatccttg acccggcgcg cccgattgcc gggctgaatg
    39181 attccaaaaa gctgagcgaa aaacgccgtc tggcgctcta tgaagagatc aaagagaaag
    39241 cgttgagctg gagtctgggc cgcgcggaac cccacgaaat cgacgagctg aacattcttc
    39301 atgcgaccat gctggcgatg cagcgtgccg tcgctgggct gcatattgcg ccggaatatg
    39361 tgttgattga tggtaaccgc tgcccgaaat taccgatgcc tgcgatggct gtggtgaaag
    39421 gcgatagccg cgtaccggaa atcagtgccg cgtctatcct ggcgaaagtg acgcgtgacg
    39481 ccgaaatggc ggcgctggat attgttttcc cgcaatatgg ttttgcccaa cacaaagggt
    39541 acccaaccgc ttttcatctg gaaaaactgg ctgaacacgg cgcgaccgaa caccatcggc
    39601 gcagctttgg gcctgtcaaa cgcgcactgg gacttgcgtc ctgattcttg tgtcgagatt
    39661 aagtaaaccg gaatctgaag atgtctgaac cacgtttcgt acacctgcgg gtgcacagcg
    39721 actactcgat gatcgatggc ctggccaaaa ccgcaccgtt ggtaaaaaag gcggcggcgt
    39781 tgggtatgcc agcactggcg atcaccgatt tcaccaacct ttgtggtctg gtgaagttct
    39841 acggagcggg acatggcgca gggattaagc ctatcgtcgg ggcagatttt aacgtccagt
    39901 gcgacctgct gggtgatgag ttaacccacc tgacggtact ggcggcgaac aataccggct
    39961 atcagaatct gacgttgctg atctcaaaag cgtatcagcg cgggtacggt gccgccgggc
    40021 cgatcatcga tcgcgactgg cttatcgaat taaacgaagg gttgatcctt ctttccggcg
    40081 gacgcatggg cgacgtcgga cgcagtcttt tgcgtggtaa cagcgcgctg gtagatgagt
    40141 gtgtcgcgtt ttatgaagaa cacttcccgg atcgctattt tctcgagctg atccgcaccg
    40201 gcaggccgga cgaagaaagc tatctgcacg cggcggtgga actggcggaa gcgcgcggtt
    40261 tgcccgtcgt ggcgaccaac gacgtgcgct ttatcgacag cagcgacttt gacgcacacg
    40321 aaatccgcgt cgcgatccac gacggcttta ccctcgacga tcctaaacgc ccgcgtaact
    40381 attcgccgca gcaatatatg cgtagcgaag aggagatgtg tgagctgttt gccgacatcc
    40441 ccgaagccct tgccaacacc gttgagatcg ccaaacgctg taacgtaacc gtgcgtcttg
    40501 gtgaatactt cctgccgcag ttcccgaccg gggacatgag caccgaagat tatctggtca
    40561 agcgtgcaaa agagggcctg gaagagcgtc tggccttttt attccctgat gaggaagaac
    40621 gtcttaagcg ccgcccggaa tatgacgaac gtctggagac tgaacttcag gttatcaacc
    40681 agatgggctt cccgggctac ttcctcatcg ttatggaatt tatccagtgg tcgaaagata
    40741 acggcgtacc ggtagggcca ggccgtggct ccggtgcggg ttcactggtg gcctacgcgc
    40801 tgaaaatcac cgacctcgat ccgctggaat ttgacctgct gttcgaacgt ttccttaacc
    40861 cggaacgtgt ctccatgcct gacttcgacg ttgacttctg tatggagaaa cgcgatcagg
    40921 ttatcgaaca cgtggcggac atgtacggtc gtgatgcggt atcgcagatc atcaccttcg
    40981 gtacaatggc ggcgaaagcg gtgatccgcg acgtaggccg cgtgctgggg catccgtacg
    41041 gctttgtcga tcgtatctcg aaactgatcc cgcccgatcc ggggatgacg ctggcgaaag
    41101 cgtttgaagc cgagccgcag ctgccggaaa tctacgaagc ggatgaagaa gttaaggcgc
    41161 tgatcgacat ggcgcgcaaa ctggaagggg tcacccgtaa cgccggtaag cacgccggtg
    41221 gggtggttat cgcgccgacc aaaattaccg attttgcgcc gctttactgc gatgaagagg
    41281 gcaaacatcc ggtcacccag tttgataaaa gcgacgttga atacgccgga ctggtgaagt
    41341 tcgacttcct tggtttgcgt acgctcacca tcatcaactg ggcgctggag atgatcaaca
    41401 agcggcgggc gaagaatggc gagccgccgc tggatatcgc cgcgatcccg ctggatgaca
    41461 agaaaagctt cgacatgctg caacgctcgg aaaccacggc ggtattccag cttgaatcgc
    41521 gcggcatgaa ggacctgatc aagcgtctgc aacctgactg cttcgaagat atgatcgcac
    41581 tggtggcgct gttccgccca ggtccgttgc aatcagggat ggtggataac tttatcgacc
    41641 gtaaacatgg tcgcgaagaa atctcctatc cggacgtaca gtggcagcat gaaagcctga
    41701 aaccggtact ggagccaacc tacggcatca tcctgtatca ggaacaggtc atgcagattg
    41761 cgcaggtgct ttctggttat accctcggtg gcgcggatat gctgcgtcgt gcgatgggta
    41821 agaaaaagcc ggaagagatg gctaagcagc gttctgtatt tgctgaaggt gcagaaaaga
    41881 acggaatcaa cgccgaactg gcgatgaaaa tcttcgacct ggtggagaaa ttcgcgggtt
    41941 acggatttaa caaatcgcac tctgcggcct atgctttggt gtcatatcaa acgttatggc
    42001 tgaaagcgca ctatccggcg gagtttatgg cggcggtaat gaccgccgat atggacaaca
    42061 ccgagaaggt ggtgggcctg gtggatgagt gctggcggat ggggctgaaa atcctgccac
    42121 cagatataaa ctccggtctt taccatttcc acgtcaacga cgacggcgaa atcgtgtatg
    42181 gtattggcgc catcaaaggg gtaggtgaag gtccgattga ggccatcatc gaagcccgta
    42241 ataaaggcgg ctacttccgc gaactgtttg atctctgcgc ccgtaccgac accaaaaagt
    42301 taaaccggcg ggtgctggaa aaactgatca tgtccggggc gtttgaccgt cttgggccac
    42361 accgcgcggc gctgatgaac tcgctgggtg atgcgttaaa agcggcagat caacacgcga
    42421 aagcggaagc tatcggtcag gccgatatgt tcggcgtgct ggccgaagag ccggaacaaa
    42481 ttgaacaatc ctacgccagc tgccaaccgt ggccggagca ggtggtatta gatggggaac
    42541 gtgaaacgtt aggcctgtac ctgaccggac accctatcaa ccagtattta aaagagattg
    42601 agcgttatgt cggaggcgta aggctgaaag acatgcaccc gacagaacgt ggtaaagtca
    42661 tcacggctgc ggggctcgtt gttgccgcgc gggttatggt caccaagcgc ggcaatcgta
    42721 tcggtatctg cacgctggat gaccgttccg ggcggctgga agtgatgttg tttactgacg
    42781 ccctggataa ataccagcaa ttgctggaaa aagaccgcat acttatcgtc agcggacagg
    42841 tcagctttga tgacttcagc ggtgggctta aaatgaccgc tcgcgaagtg atggatattg
    42901 acgaagcccg ggaaaaatat gctcgcgggc ttgctatctc gctgacggac aggcaaattg
    42961 atgaccagct tttaaaccga ctccgtcagt ctctggaacc ccaccgctct gggacaattc
    43021 cagtacatct ctactatcag agggcggatg cacgcgcgcg gttgcgtttt ggcgcgacgt
    43081 ggcgtgtctc tccgagcgat cgtttattaa acgatctccg tggcctcatt ggttcggagc
    43141 aggtggaact ggagtttgac taatacagga atactatgag tctgaatttc cttgattttg
    43201 aacagccgat tgcagagctg gaagcgaaaa tcgattctct gactgcggtt agccgtcagg
    43261 atgagaaact ggatattaac atcgatgaag aagtgcatcg tctgcgtgaa aaaagcgtag
    43321 aactgacgcg taaaatcttc gccgatcttg gtgcatggca gattgcgcaa ctggcacgcc
    43381 atccacagcg tccttatacc ctggattacg ttcgcctggc atttgatgaa tttgacgaac
    43441 tggctggcga ccgcgcgtat gcagacgata aagctatcgt cggtggtatc gcccgtctcg
    43501 atggtcgtcc ggtgatgatc attggtcatc aaaaaggtcg tgaaaccaaa gaaaaaattc
    43561 gccgtaactt tggtatgcca gcgccagaag gttaccgcaa agcactgcgt ctgatgcaaa
    43621 tggctgaacg ctttaagatg ccaatcatca cctttatcga caccccgggg gcttatccgg
    43681 gcgtgggcgc agaagaacgt ggtcagtctg aagccattgc acgcaacctg cgtgaaatgt
    43741 ctcgcctcgg cgtaccggta gtttgtacgg ttatcggtga aggtggttct ggcggtgcgc
    43801 tggcgattgg cgtgggcgat aaagtgaata tgctgcaata cagcacctat tccgttatct
    43861 cgccggaagg ttgtgcgtcc attctgtgga agagcgccga caaagcgccg ctggcggctg
    43921 aagcgatggg tatcatcgct ccgcgtctga aagagctgaa actgatcgac tccatcatcc
    43981 cggaaccgct gggtggcgct caccgtaacc cggaagcgat ggcggcatcg ttgaaagcgc
    44041 aactgctggc ggatctggcc gatctcgatg tgttaagcac tgaagattta aaaaatcgtc
    44101 gttatcagcg cctgatgagc tacggttacg cgtaattcgc aaaagttctg aaaaagggtc
    44161 acttcggtgg ccctttttta tcgccacggt ttgagcaggc tatgattaag gaaggatttt
    44221 ccaggaggaa cacatgaaca tcattgccat tatgggaccg catggcgtct tttataaaga
    44281 tgagcccatc aaagaactgg agtcggcgct ggtggcgcaa ggctttcaga ttatctggcc
    44341 acaaaacagc gttgatttgc tgaaatttat cgaacataac cctcgaattt gcggcgtgat
    44401 ttttgactgg gatgagtaca gtctcgattt atgcagcgat atcaatcaac ttaatgaata
    44461 tctcccgctt tatgccttca tcaacaccca ctcgacgatg gatgtcagcg tgcaggatat
    44521 gcggatggcg ctctggtttt ttgaatatgc gctggggcag gcggaagata tcgccattcg
    44581 tatgcgtcag tacaccgacg aatatcttga taacattaca ccgccgttca cgaaagcctt
    44641 gtttacctac gttaaagagc ggaagtacac cttttgtacg ccggggcata tgggcggcac
    44701 cgcatatcaa aaaagcccgg ttggctgcct gttttatgat tttttcggcg ggaataccct
    44761 caaggctgat gtctctattt cggtcaccga gcttggttcg ttgctcgacc acaccgggcc
    44821 acacctggaa gcggaagagt acatcgcgcg gacttttggc gcggaacaga gttatatcgt
    44881 taccaacgga acatcgacgt cgaacaaaat tgtgggtatg tacgccgcgc catccggcag
    44941 tacgctgttg atcgaccgca attgtcataa atcgctggcg catctgttga tgatgaacga
    45001 tgtagtgcca gtctggctga aaccgacgcg taatgcgttg gggattcttg gtgggatccc
    45061 gcgccgtgaa tttactcgcg acagcatcga agagaaagtc gctgccacca cgcaggcaca
    45121 atggccggtt catgcggtga ttaccaactc cacctatgat ggcttgctct acaacaccga
    45181 ctggatcaaa cagacgctgg atgtcccgtc gatccacttc gattccgcct gggtgccgta
    45241 cacccatttt catccgatct accaggggaa aagtggtatg agcggcgagc gtgttgcggg
    45301 aaaagtgatc ttcgaaacgc aatcgaccca caaaatgctg gcggcgttat cgcaggcttc
    45361 gctgatccac attaaaggcg agtatgacga agaggcgttt aacgaagcct ttatgatgca
    45421 taccaccacc tcgcccagtt atcccattgt tgcttcggtt gagacggcgg cggcgatgct
    45481 gcgtggtaat ccgggcaaac ggctgattaa ccgttcggtg gagcgggcgc tgcattttcg
    45541 caaagaggtc cagcggttgc gggaagagtc tgacggttgg tttttcgata tctggcaacc
    45601 gccgcaggtg gatgaagccg aatgctggcc cgttgcgcca ggcgaacagt ggcacggctt
    45661 taacgatgcg gatgccgatc atatgtttct cgatccggtt aaagtcacta ttttgacacc
    45721 ggggatggac gaacagggca atatgagtga ggaggggatc ccggcggcgc tggtggcaaa
    45781 attcctcgac gaacgtggga tcgtagtaga gaaaaccggc ccttataacc tgctgtttct
    45841 ctttagtatt ggtatcgata aaaccaaagc aatgggatta ttgcgtgggt tgacggaatt
    45901 taaacgctct tacgatctca acctgcggat caaaaatatg ctacccgatc tctatgctga
    45961 agatcccgat ttctaccgca atatgcgtat tcaggatctg gcgcaaggga tccataagct
    46021 gattcgtaaa cacgatcttc ccggtttgat gttgcgggca ttcgatactt tgccggaaat
    46081 gatcatgacg ccacatcagg catggcagag acagattaaa ggtgaagtag aaaccattgc
    46141 gctggaacaa ctggtcggta gagtatcggc aaatatgatc ctgccttatc caccgggcgt
    46201 accgctgttg atgccaggag aaatgctgac caaagagagc cgcacggtac tcgattttct
    46261 actgatgctt tgttccgtag ggcaacatta ccccggtttt gaaacggata ttcacggcgc
    46321 gaaacaggac gaagacggcg tttaccgcgt acgagtccta aaaatggcgg gataacttgc
    46381 cagagcggct tccgggcgag taacgtgctg ttaacaaata aaggagacgt tatgctgggt
    46441 ttaaaacagg ttcaccatat tgcgattatt gcgacggatt atgcggtgag caaagctttc
    46501 tactgcgata ttcttggttt cacgctgcaa agcgaagtct atcgcgaagc gcgcgactcg
    46561 tggaaagggg atttggcgct taatgggcaa tatgtgattg agcttttctc ttttccgttc
    46621 ccgccggagc gccccagccg accggaagct tgcggcctgc gtcatctggc ttttagcgtt
    46681 gatgacatcg atgcggcagt ggcgcacctt gaaagccata acgtgaagtg tgaagccatc
    46741 cgtgtcgatc catacacgca aaaacgcttc accttcttta acgatccgga cgggctgccg
    46801 ttggaactgt atgagcagta aggcttgtca tcgccgcatt tgcccggtaa cgtgccgggc
    46861 attgctactg taaaatcgca ccatcatgac actcacgctc aatagacaac ttctcacctc
    46921 acgccagatt ctggtggcct ttagcggcgg gcttgactcc accgttctgc tgcatcagtt
    46981 ggtgcagtgg cggacggaaa atccgggcgt cactctgcgc gctattcatg tgcatcacgg
    47041 tttaagtgcc aatgccgatg cctgggttac gcattgcgaa aacgtctgcc aacagtggca
    47101 ggtgccgctg gtggtcgaac gcgtacaact tgcgcaagag gggctgggga ttgaggccca
    47161 agcgcggcag gcgcgttatc aggcatttgc ccgtacccta ttgcccggcg aagtgctggt
    47221 caccgcgcaa catctcgacg atcaatgtga aacctttctg ctggcgctaa aacgcggtag
    47281 cggccctgcc gggctttcgg ctatggggga agtctcggag tttgccggaa cacggcttat
    47341 ccgcccgttg cttgcccgca cgcgggggga actggcgcag tgggcattgg cgcatggttt
    47401 acgctggatt gaagacgaaa gtaatcagga cgactcatac gatcgtaact ttctgcgcct
    47461 gcgtgtagtg ccgttattgc agcagcgttg gccgcatttt gccgaagcaa cggcccgcag
    47521 cgccgcactt tgtgctgaac aagagagtct gttggatgaa ctgctggcgg atgatttagc
    47581 acactgccaa acgtcgcagg ggacgctgca gattgcgcca atgctagcga tgagtgatgc
    47641 ccgtcgcgcg gcgattatcc gccgctggct ggcagggcag aatgcaccga tgccttcccg
    47701 cgacgcgctg gtgcggatct ggcaggaagt ggcgctggcg cgggaagatg cctccccctg
    47761 tttacgcttg ggcgcgtttg aaatccgccg atatcagtcg caactgtggt ggattaaatc
    47821 cgtcaccggg caaagtgaaa ccattgtgcc gtggcagacg tggctacaac cgctggaatt
    47881 accggcgggg ctgggaagcg tacagcttac tgcgggaggc gatattcgcc ctccgcgtgc
    47941 agacgaagcg gtcagcgtgc gtttcaaagc ggcaggattg ctgcatattg tcgggcgcaa
    48001 cggcggacgt aagctgaaga aaatctggca agagttaggc gtgccgccgt ggctacgtga
    48061 caccacgcca ctgctgtttt atggcgaaac gctgattgcg gcggcagggg tatttgtgac
    48121 gcaagaaggt gtggctgaag gtgagaatgg cgtaagtttt gtctggcaga aaacgcttag
    48181 ttaagtgaaa gccggataag acgcatcaaa cgtcgcatcc ggcgaaagtc aatcaggact
    48241 cgctcaccac caccgtaccg atttccgggt ggctaaagct ggtaatttta tccagacgta
    48301 gctcacgggt ttcgccagcg gcctcgacga ccagatactc cacatttttg cgggagacta
    48361 aatcgctggc ttttgcctgc aatttttcgc catctttcag ctcaagtgtc agcattaaat
    48421 gatgctggca ggcgagctca agattatcgt aatcatcaca attgattggt tgatacgtat
    48481 cattcattga cataatcgct caccagtaag tttgccgcag cgtatgctgc tttttccctg
    48541 acagcctcag aaagggcgtc gtcggcagcc atttcattca gcactttcaa aacgcagccc
    48601 agcgcgtccg gaacgtatcc taagtctccg ctggcgattt ccgcgtaccg cttgcgtatt
    48661 aactcacaat atttttccac atgccctcct gtcagcactc tgacttaacc gtggatgcaa
    48721 gtctaagcct acgaagataa actctgtttc gcaaggtgac tataccacac tcatttctgc
    48781 aatatcagcg ccgcaactgc acgtattccg ttacaatggc ctcctgattc gaaaggagtt
    48841 ttcttatggc gcttaaagcg acaatttata aagcgacggt taatgtggcc gatctcgacc
    48901 gcaaccagtt tctcgatgcc tctctgacgc tggcgcgcca cccttcagaa acccaggagc
    48961 gtatgatgct gcgcttgctg gcgtggctga aatatgctga tgaacgtctg caatttaccc
    49021 gtggtttgtg tgccgatgat gagccggaag cgtggctgcg taacgatcac ctgggcattg
    49081 atttgtggat tgagctgggg ctgccggatg agcggcggat taagaaagcc tgcacccagg
    49141 ccgcagaagt ggcgctgttt acctataata gtcgggcggc gcaaatctgg tggcagcaaa
    49201 atcagagcaa atgtgtgcag tttgccaatc tttccgtctg gtatctggac gatgaacaac
    49261 tggcgaaagt aagcgccttt gccgatcgta ccatgacgct gcaggcaacg attcaggatg
    49321 gcgtgatctg gttatcggac gataagaata atctggaagt taacttaacc gcctggcaac
    49381 aaccttcatg attgtgattt cccgacatgt tgctattccc gatggtgagc ttgagatcac
    49441 cgccattcgt gcgcagggcg cgggcgggca gcatgttaat aagacctcaa cggccattca
    49501 tctgcgtttt gacattcggg cgtccagcct gccagagtat tacaaagagc gtctactcgc
    49561 cgccagccat catttgatca gcagtgatgg cgtgattgtc attaaggcac aggaataccg
    49621 cagtcaggaa ctgaaccgcg aagccgcgct agcccggctg gtggcagtga ttaaagattt
    49681 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
    49741 gctggcatcg aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
    49801 tcgggaataa aaagaaggaa tagatggtga aaaaagcgat agtgacagcg atggctgtaa
    49861 tcagcctctt tactctgatg ggatgtaata accgggccga agtcgatacg ctttctccag
    49921 cacaggctgc cgaactgaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgctg
    49981 attgcgaagg aatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
    50041 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
    50101 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
    50161 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc gattgaatcg cagttcaact
    50221 atacgctgga accggcacaa tctagtttac ctatgacgcc gatgaccctg cgaggcatgt
    50281 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
    50341 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
    50401 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
    50461 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
    50521 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
    50581 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
    50641 taaggcgttt acgccgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
    50701 cccaacaaag cgacccgcct tgatataaat cccaggcttc aggtgcccaa catactgtaa
    50761 ctgcgtttct ggcttaaacg cagagacatc accaatacgc atatgcatca gatcggcagg
    50821 gctaatccag cccgattgcg ccagtgtaag tggatgcccg gctttggcaa aggcatccgt
    50881 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
    50941 tttcgccagg ccgctgacgc actttggcga aaatcctcgg agaacggatt cagtgagcac
    51001 atctggcgag tcaccataaa gggaataaat tcgacaatgc cgcgatagtt ataaccgcta
    51061 tctttgattt tattggcaaa cgcggtgata tctgtggctt gttgcggggt aagatccggg
    51121 actcgtaaga cgaaaagctt atcactatgc ttcatcgctt ttttaaggga aacaatctgg
    51181 acgccagcgc ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc aacgtgactc
    51241 acagaggaag tgctgaagac gcggattcca aatgaggtta ccccaaggct tgaggagaac
    51301 agcaaatcgc cgggtttgag atcaggtgcc gttatttctt ttattgattg ttcggtgaaa
    51361 gagctttgat gctggaattt gacagcccat gtttttgcct cggcatctac tgctgtggct
    51421 gatgggtctg gctggctgat atcaacggta caagccgaaa gtaaaagaaa gctggggaga
    51481 aacaggcggc agtacgcctt tggtttatcc attttataca atccatgtaa aaaaagggcc
    51541 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
    51601 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
    51661 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
    51721 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
    51781 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
    51841 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgcctcgtt
    51901 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
    51961 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
    52021 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
    52081 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
    52141 gggtagcgac atcgcgatcc cagttgatgc cgaagtagtg tttaccatcg atgttagcac
    52201 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
    52261 ttaccggacc cagtgaaccc ggaccggctt taaccacggc acgaatttct tcttcggtcg
    52321 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
    52381 gatcaccgcg caccagcagc gcaaccagcg ggaagctact gccttcaacc gctttaacca
    52441 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
    52501 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
    52561 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
    52621 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
    52681 tagaaccggt gtcggcttgt acggcgcgga aatccagccc catgcggctg aagattttgc
    52741 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
    52801 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
    52861 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
    52921 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
    52981 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
    53041 cttgccacaa atcagctggc tgaaccaccg gcatcgacac ctcgatcgca ccggcgttgt
    53101 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
    53161 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
    53221 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
    53281 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
    53341 aaaagtgatt tagtttacca gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac
    53401 gcggttccag cgcaaagact tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat
    53461 catgtagcca gacggcatag gttttgcccg tttcctcacc tttacgatag gccgggcgtg
    53521 ggtcctgcgc cagtacttcg cggataaaca gcgttaactg cggataacgc ttctccagcg
    53581 tcaaaagctg cttttcgacc tctgcggtaa aactcaccgc catctctgcc gctggcgcac
    53641 tttgcgcata gctggcactg gcatcgggaa gcgattcggc aaaggggaga tacggtttga
    53701 tatccactac cggcgtacca tcgaccagat ccagactgcc gagcttcaga atcacgctgt
    53761 ctttatggca aacaacctct ttcagctcta ccagcgacat accaattggg ttagggcgga
    53821 aagtagagcg tgttgcgaaa acccccattc tggcgttacc gccgaggcgc ggtggacgca
    53881 cagtcggacg ccagccgcct tccatcgttt gatgaaagac gaaaaggatc cataaatggc
    53941 tgaacgcttc caggccgcga acggcgtcgg cctggttgta gggagcaatg agatgcagtt
    54001 cgccgttggc gctttttacc agacctggct ggcgcggaac ggcgaatttt tctttatagg
    54061 gcgagcgaat aacgcctatt tgctcgaact ggaaactgct catttcgccg taatgttaag
    54121 cgcagagccg atacatacag cctgacgata gcagcctggc gtaccgctgg tgacttcgca
    54181 gctatgcagt aataccgcat tggccttcat tttcgaggcg ttgatttgca tccgcttacg
    54241 cgcggttgga atgctcggcg gagagtcctg attagaggcc tggcaagagt cgccactgac
    54301 ttcaccgaga tcgcggaacg gtttgccgac taattcttct gcattggtat aaattcggac
    54361 cggcgtggcg cgcggcgctt tcggttttgc aggctccgct ttcggctggg gtgcagtgct
    54421 ttgaacgggt tcgacagggg atctgcttaa catggaacag ccgcttagca tgagtgctac
    54481 taaacagatc ggtaaagcac gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt
    54541 gaatcaggag cttgtaaaaa tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa
    54601 attgtggaaa cagcctaaaa attaccagcc tttaacagcg ccgccgttaa acactttgtt
    54661 tgctgcttcg taaacttcat cagactgata agcctggacg aatttcttca cgttctcggc
    54721 gtctttgtta tcttcacgcg ttacgatcag gtttacgtac ggggagtctt tatcttcgac
    54781 aaagataccg tctttcgctg gagtcaggcc aatctggctg gcataggtgg tattgataac
    54841 tgccagagcg atttgcgcgt cgtccagaga tcgaggcagc tgcggtgctt ccagctcaac
    54901 aattttcaga ttttttgggt tctcaacaac atcaagaacg gtcggcagca ggccaacgcc
    54961 atctttcagt ttgatcaagc ccactttttg cagcagcagc agtgaacgac caagattagt
    55021 tggatcgttt ggcacggcaa cctgcgaacc atcctgcagt tcatccagtg atttgatttt
    55081 cttggagtaa ccagcaatcg gataaacaaa agtgttgcct actgcgacca gtttgtagcc
    55141 acgatctttc agttgctgat caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc
    55201 gcctttgctc aatgcttcgt ttggcagaac atagtcgttg aaggttacca gctcaacgtc
    55261 caggccatat ttgtctttcg caactttctg cgcgacttct gctacctgct gttcggcacc
    55321 aacaatcacg ccgactttaa tgtggtttgg atctttttca tcctgaccgc agcctaccag
    55381 tgccagtgat ccaatcaggg ctcccactgc cgcaaaggtt ttgaatttga acgccatacc
    55441 ttattccttc ttcaattatt tatgttgtgt tgaacgttac ttgcgagtga cagcccggac
    55501 gatgcggtcg cctgcgaact gaattaaata aaccagaatg accagcaata ccagtaccgt
    55561 attcatcacc gtcgcgttat agccgatgta gccatactga tagccaatct gacctaaacc
    55621 accggcaccg actgcaccac ccatcgcgga ataaccgacc agggtaatca gggtgatagt
    55681 tgccgcattc accagacccg gcaatgcttc cggtaacagc actttacgga cgatctgcat
    55741 cggcgtggca cccattgcac gggaagcttc aattaacccg gttgggatct ccagcagagc
    55801 gttctcgacc atacgggcaa taaacggtgc tgcaccaacg gttaacggaa caatcgctgc
    55861 ctgcaaacca atcgatgtac cgacaataac gcgggtaaac ggaatcatcc ataccagcaa
    55921 gataatgaac gggatggaac ggaaaatgtt cacaatcgca gaaacggtac gatacagctt
    55981 cgcgttagca ataatttgcc ccgggcgcgt aacataaagc aggacgccga ccggcagacc
    56041 aatcacaaag ccaaaaaatc cggatacgaa ggtcattgcc agcgtttccc atacgccacg
    56101 aaccagcaac cacatcatcg gctcagacat aacccagtac ctctactttt acatggtgtt
    56161 cctgcagcca ggcaatggcg gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca
    56221 tgatgccgaa cttaacgcca ccggcgtaat ccatctgcgc gctaataatg ttgttgttga
    56281 cgttgaaacg acgcgcggtt tcagaaagca gcggggcatc gaccgattga ccggtaaact
    56341 ccaggcgcaa catcggcacg cagtcagtaa atggctccgc ttgcagacgt tcctggtaat
    56401 cttccgggat atccagatgc agggtcgact gaataaactt ctgcgccagc ggcgttttcg
    56461 gatgcgagaa cacttcactt accgtgtcct gctcgatcag ttcgccattg ctgatgaccg
    56521 ccacgcaatc acaaatacgc ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca
    56581 aacccagacg gcggttaatg tctttcagca gttcgagaat agaacgtgtc gttgccgggt
    56641 ccagcgcgct ggtggcttca tcacacagca atactttggg attgctggct aatgcacggg
    56701 caatcgccac acgttgtttc tgcccaccgg aaagattcga cgggtagcta tcatgcttat
    56761 cgccaagacc aaccaatgac agcaattccg tcacgcgacg tttgatctcg tctttcggtg
    56821 tgttgtccag ctccagcggc agagccacgt tgccaaaaac agtacgcgaa gagagcaggt
    56881 taaaatgctg gaaaatcata ccaatctggc ggcgagcttt ggtcaactcg gattctgaca
    56941 gcgtggtcag ttcctggcca tcgaccagca cgctaccctc ggttgggcgc tccagcaggt
    57001 ttacacaacg tataagcgta ctcttacccg cgcctgaggc accgataacg ccataaattt
    57061 gtccagctgg cacatgcagg ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt
    57121 ggaacacttt ggtgatattc gaaagtttta tcattgatta tttattatcg tcattaagtt
    57181 agtcgtggca tttcgaatgc ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc
    57241 atccagacgt ctaaatcaat caggtttatg cgaagagcac tttcttgcag gtcgaaacat
    57301 gcgatactag cgtcacatgc cttattaagg agctataaaa ggtggcgaag agcgtacccg
    57361 caatttttct tgaccgtgat ggcaccatta atgtcgatca cggctatgtc catgagatcg
    57421 acaactttga atttatcgac ggtgttattg acgccatgcg cgagctaaaa aaaatgggct
    57481 ttgcgctggt ggtagtaacc aaccagtctg gcattgctcg cggtaaattt actgaagcgc
    57541 agtttgaaac gctgaccgag tggatggact ggtcgctggc ggaccgcgat gtcgatctgg
    57601 atggcatcta ttattgcccg catcatccgc agggtagtgt tgaagagttt cgccaggtct
    57661 gcgactgccg caaaccacat ccggggatgc ttttgtcagc acgcgattat ttgcatattg
    57721 atatggccgc ttcttatatg gtgggcgata aattagaaga tatgcaggca gcggctgcgg
    57781 cgaacgtggg aacaaaagtg ctggtgcgta cgggtaaacc tattacgcct gaagcagaaa
    57841 acgcggcgga ttgggtgtta aatagcctgg cagacctgcc gcaagcgata aaaaagcagc
    57901 aaaaaccggc ataatgatta aaagatgagc aattgaaata aaaatgcatt tttccacttg
    57961 tcttcctgag acgactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc
    58021 acacaaacag ccggttcggt tgaagagaaa aatcc
//
LOCUS       DAPGWD010000013        52598 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPGWD010000013 DAPGWD010000000
VERSION     DAPGWD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.013"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            190..684
                     /gene="napF"
                     /locus_tag="RHX88_000737"
     CDS             190..684
                     /gene="napF"
                     /locus_tag="RHX88_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDV2229467.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAMIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            674..937
                     /gene="napD"
                     /locus_tag="RHX88_000738"
     CDS             674..937
                     /gene="napD"
                     /locus_tag="RHX88_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDV2229468.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            934..3420
                     /gene="napA"
                     /locus_tag="RHX88_000739"
     CDS             934..3420
                     /gene="napA"
                     /locus_tag="RHX88_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDV2229469.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3427..4122
                     /gene="napG"
                     /locus_tag="RHX88_000740"
     CDS             3427..4122
                     /gene="napG"
                     /locus_tag="RHX88_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDV2229470.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4109..4972
                     /gene="napH"
                     /locus_tag="RHX88_000741"
     CDS             4109..4972
                     /gene="napH"
                     /locus_tag="RHX88_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDV2229471.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4969..5418
                     /gene="napB"
                     /locus_tag="RHX88_000742"
     CDS             4969..5418
                     /gene="napB"
                     /locus_tag="RHX88_000742"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDV2229472.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5428..6030
                     /gene="napC"
                     /locus_tag="RHX88_000743"
     CDS             5428..6030
                     /gene="napC"
                     /locus_tag="RHX88_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDV2229473.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6049..6666
                     /gene="ccmA"
                     /locus_tag="RHX88_000744"
     CDS             6049..6666
                     /gene="ccmA"
                     /locus_tag="RHX88_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDV2229474.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6663..7325
                     /gene="ccmB"
                     /locus_tag="RHX88_000745"
     CDS             6663..7325
                     /gene="ccmB"
                     /locus_tag="RHX88_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDV2229475.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7367..8104
                     /gene="ccmC"
                     /locus_tag="RHX88_000746"
     CDS             7367..8104
                     /gene="ccmC"
                     /locus_tag="RHX88_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDV2229476.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8101..8310
                     /gene="ccmD"
                     /locus_tag="RHX88_000747"
     CDS             8101..8310
                     /gene="ccmD"
                     /locus_tag="RHX88_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDV2229477.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8307..8786
                     /gene="ccmE"
                     /locus_tag="RHX88_000748"
     CDS             8307..8786
                     /gene="ccmE"
                     /locus_tag="RHX88_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDV2229478.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8783..10726
                     /gene="ccmF"
                     /locus_tag="RHX88_000749"
     CDS             8783..10726
                     /gene="ccmF"
                     /locus_tag="RHX88_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDV2229479.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10723..11280
                     /gene="dsbE"
                     /locus_tag="RHX88_000750"
     CDS             10723..11280
                     /gene="dsbE"
                     /locus_tag="RHX88_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDV2229480.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11277..12329
                     /gene="ccmH"
                     /locus_tag="RHX88_000751"
     CDS             11277..12329
                     /gene="ccmH"
                     /locus_tag="RHX88_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDV2229481.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12364..13011)
                     /gene="narP"
                     /locus_tag="RHX88_000752"
     CDS             complement(12364..13011)
                     /gene="narP"
                     /locus_tag="RHX88_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDV2229482.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13331..15922
                     /gene="yejO"
                     /locus_tag="RHX88_000753"
                     /pseudo
     CDS             13331..15922
                     /gene="yejO"
                     /locus_tag="RHX88_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(16026..16102)
                     /locus_tag="RHX88_000754"
     tRNA            complement(16026..16102)
                     /locus_tag="RHX88_000754"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16066..16068),aa:Pro,seq:ggg)
     gene            complement(16177..17937)
                     /gene="pbgA"
                     /locus_tag="RHX88_000755"
     CDS             complement(16177..17937)
                     /gene="pbgA"
                     /locus_tag="RHX88_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDV2229483.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(17957..18184)
                     /locus_tag="RHX88_000756"
     CDS             complement(17957..18184)
                     /locus_tag="RHX88_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDV2229484.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            18366..19373
                     /gene="yejK"
                     /locus_tag="RHX88_000757"
     CDS             18366..19373
                     /gene="yejK"
                     /locus_tag="RHX88_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDV2229485.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(19512..19796)
                     /gene="rplY"
                     /locus_tag="RHX88_000758"
     CDS             complement(19512..19796)
                     /gene="rplY"
                     /locus_tag="RHX88_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDV2229486.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(19921..21681)
                     /locus_tag="RHX88_000759"
     CDS             complement(19921..21681)
                     /locus_tag="RHX88_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV2229487.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            21830..22525
                     /gene="rsuA"
                     /locus_tag="RHX88_000760"
     CDS             21830..22525
                     /gene="rsuA"
                     /locus_tag="RHX88_000760"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDV2229488.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            22553..23743
                     /gene="bcr"
                     /locus_tag="RHX88_000761"
     CDS             22553..23743
                     /gene="bcr"
                     /locus_tag="RHX88_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDV2229489.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            24076..24420
                     /locus_tag="RHX88_000762"
     CDS             24076..24420
                     /locus_tag="RHX88_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229490.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(24424..26013)
                     /gene="yejF"
                     /locus_tag="RHX88_000763"
     CDS             complement(24424..26013)
                     /gene="yejF"
                     /locus_tag="RHX88_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDV2229491.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGAPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(26015..27040)
                     /locus_tag="RHX88_000764"
     CDS             complement(26015..27040)
                     /locus_tag="RHX88_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2229492.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(27040..28134)
                     /locus_tag="RHX88_000765"
     CDS             complement(27040..28134)
                     /locus_tag="RHX88_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDV2229493.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGGRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(28135..29949)
                     /locus_tag="RHX88_000766"
     CDS             complement(28135..29949)
                     /locus_tag="RHX88_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2229494.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKNHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            complement(30031..31587)
                     /locus_tag="RHX88_000767"
     CDS             complement(30031..31587)
                     /locus_tag="RHX88_000767"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV2229495.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(31768..32334)
                     /gene="mepS"
                     /locus_tag="RHX88_000768"
     CDS             complement(31768..32334)
                     /gene="mepS"
                     /locus_tag="RHX88_000768"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDV2229496.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(32746..33459)
                     /gene="lpxT"
                     /locus_tag="RHX88_000769"
     CDS             complement(32746..33459)
                     /gene="lpxT"
                     /locus_tag="RHX88_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDV2229497.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(33498..34484)
                     /gene="yeiR"
                     /locus_tag="RHX88_000770"
     CDS             complement(33498..34484)
                     /gene="yeiR"
                     /locus_tag="RHX88_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDV2229498.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNGNFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(34602..36068)
                     /locus_tag="RHX88_000771"
     CDS             complement(34602..36068)
                     /locus_tag="RHX88_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV2229499.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(36291..36863)
                     /gene="yeiP"
                     /locus_tag="RHX88_000772"
     CDS             complement(36291..36863)
                     /gene="yeiP"
                     /locus_tag="RHX88_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDV2229500.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            37018..37272
                     /locus_tag="RHX88_000773"
     CDS             37018..37272
                     /locus_tag="RHX88_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV2229501.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(37269..38450)
                     /gene="setB"
                     /locus_tag="RHX88_000774"
     CDS             complement(37269..38450)
                     /gene="setB"
                     /locus_tag="RHX88_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDV2229502.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            38818..39948
                     /gene="fruB"
                     /locus_tag="RHX88_000775"
     CDS             38818..39948
                     /gene="fruB"
                     /locus_tag="RHX88_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDV2229503.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            39948..40886
                     /gene="fruK"
                     /locus_tag="RHX88_000776"
     CDS             39948..40886
                     /gene="fruK"
                     /locus_tag="RHX88_000776"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDV2229504.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            40903..42594
                     /gene="fruA"
                     /locus_tag="RHX88_000777"
     CDS             40903..42594
                     /gene="fruA"
                     /locus_tag="RHX88_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDV2229505.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            43018..43959
                     /locus_tag="RHX88_000778"
     CDS             43018..43959
                     /locus_tag="RHX88_000778"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDV2229506.1"
                     /translation="MREKNYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            43947..44885
                     /gene="psuG"
                     /locus_tag="RHX88_000779"
     CDS             43947..44885
                     /gene="psuG"
                     /locus_tag="RHX88_000779"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDV2229507.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            44979..46229
                     /locus_tag="RHX88_000780"
     CDS             44979..46229
                     /locus_tag="RHX88_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV2229508.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQHEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(46297..46995)
                     /gene="yeiL"
                     /locus_tag="RHX88_000781"
     CDS             complement(46297..46995)
                     /gene="yeiL"
                     /locus_tag="RHX88_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDV2229509.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            47125..48066
                     /gene="rihB"
                     /locus_tag="RHX88_000782"
     CDS             47125..48066
                     /gene="rihB"
                     /locus_tag="RHX88_000782"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDV2229510.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            48166..49416
                     /locus_tag="RHX88_000783"
     CDS             48166..49416
                     /locus_tag="RHX88_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV2229511.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNISFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(49472..50560)
                     /locus_tag="RHX88_000784"
     CDS             complement(49472..50560)
                     /locus_tag="RHX88_000784"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV2229512.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCIVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(50563..51420)
                     /gene="nfo"
                     /locus_tag="RHX88_000785"
     CDS             complement(50563..51420)
                     /gene="nfo"
                     /locus_tag="RHX88_000785"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDV2229513.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(51494..52543)
                     /locus_tag="RHX88_000786"
     CDS             complement(51494..52543)
                     /locus_tag="RHX88_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDV2229514.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
BASE COUNT        12636 a        12947 c        14213 g        12802 t
ORIGIN      
        1 gtgagcaaaa gcccgttttt gccacgcttc ccgccactct tttgatcctg ctacaggttt
       61 taccccgatc ggggtatgca tctttgacac atcctttaat atcttagcgg ctataaaaat
      121 ggcttattaa ttatgcggtt tatttggccg ctctcaattt tcagagcgcg ttaatgatgg
      181 aaggtcaatg tgaagattga tgcatcccgt cggggcatac tcactggtcg ctggcgcaaa
      241 gccagtaacg gtatccgtcc gccttggtcc ggtgatgaat ctcattttct gacccattgc
      301 acccgttgcg acgcctgtat taatgcttgt gaaaacaaca ttctgcaacg cggcgcgggc
      361 ggctatccga gcgttaattt caaaaataac gagtgcagct tctgttacgc ctgcgcgcag
      421 gcttgccccg aatcgctatt ttctccgcgc cacaccaggg cctgggattt gcagttcacc
      481 attggggacg cgtgcctggc gtatcagtca gttgaatgcc gccgctgtca ggatagctgt
      541 gagccaatgg caatgatctt tcgcccgacg ctctccggga tctaccagcc gcaacttaat
      601 agccaacttt gcaacggatg tggagcatgt gcagccagct gtccggtatc agccataact
      661 gcggagtatc tccatgcaca ctaactggca agtttgcagc ctggtcgtgc aggccaaaag
      721 cgaacgaatt tcagacatca gcacccaact gaacgccttt cccggctgtg aagttgctgt
      781 cagcgacgcg ccgagcggtc agttgattgt ggtggtggaa gcagaagaca gcgaaacgct
      841 gatccaaacc attgagtcag tacgcaacgt agagggcgtg ctggcggtgt cgctggttta
      901 tcaccagcag gaagagcaag gtgaggaaac accatgaaac tcagtcgtcg tagctttatg
      961 aaagctaacg ccgttgcggc cgctgcggcg gctgccggtc tcagcgtgcc gggcgttgcc
     1021 cgcgccgttg ttggtcagca ggaagccatt aaatgggata aagcgccgtg ccgtttctgc
     1081 ggtactggtt gcggcgttct ggtcggaacg cagcaggggc gtgtggtggc ctgtcagggc
     1141 gacccggacg caccggttaa ccgtggcctg aactgcatta agggctattt cctgcccaag
     1201 atcatgtacg gtaaagaccg tttgacgcag ccgctgctgc gtatgaaaaa cggtaaatat
     1261 gacaaagaag gcgaatttac cccaatcacc tgggatcagg ccttcgatgt gatggaagag
     1321 aaattcaaaa ccgccctgaa agaaaaagga ccggaatcga tcggtatgtt cggttctggt
     1381 cagtggacta tctgggaagg ttatgccgcg tccaagctgt tcaaagcggg cttccgttcg
     1441 aacaacatcg acccgaacgc acgtcactgt atggcgtcgg cagtagttgg ctttatgcgt
     1501 acctttggta tggatgagcc gatgggctgc tatgacgaca tcgagcaggc tgacgcgttt
     1561 gtgctgtggg gcgctaacat ggcggagatg cacccgatcc tctggtcacg catcactaac
     1621 cgtcgtctct ctaaccagaa cgtcaccgtg gcggtgcttt ctacctacca gcatcgtagc
     1681 ttcgagctgg cggataacgg catcatcttt acgccacagt ctgacctggt gatcctgaac
     1741 tacatcgcca actatatcat tcaaaacaat gcgataaatc aggacttctt cagcaagcac
     1801 gttaacctgc gcaaaggggc gacggacatc ggctacggtt tacgtccgac ccatccgctg
     1861 gaaaaagcag cgaagaatcc gggttctgac gcctccgaac cgatgagctt tgaagattac
     1921 aaagccttcg ttgccgagta tacgctggaa aaaactgccg aaatgaccgg cgtgccgaaa
     1981 gaccagttag aacaactggc gcagctgtat gccgatccga acaagaaagt catctcctac
     2041 tggacgatgg gcttcaacca gcatactcgt ggcgtgtggg ccaacaacct ggtctacaac
     2101 ctgcacctgc tgaccggcaa aatttcccag ccgggttgcg gtccgttctc cctgaccggg
     2161 cagccttccg cgtgtggtac tgcgcgtgaa gtgggcacct ttgctcaccg tctgcctgcg
     2221 gacatggtgg tgactaacga gaaacaccgt gatatttgcg agaagaagtg gaatatcccg
     2281 agcggcacca ttccggcgaa aatcggtctg cacgcggtgg cacaagaccg tgcactgaaa
     2341 gacggcaagc tgaatgttta ctggaccatg tgtaccaaca acatgcaggc cgggccgaac
     2401 attaatgaag agcgtatgcc gggctggcgc gatccgcgca acttcatcat cgtctccgat
     2461 ccgtatccga cagtcagtgc gctggcagcc gacttgatcc tgccgaccgc aatgtgggta
     2521 gagaaagagg gcgcttacgg taacgccgaa cgccgtactc agttctggcg tcagcaggta
     2581 caggcgccag gcgaagcgaa atcggatctc tggcagttag tccagttctc ccgccgcttc
     2641 aaaactgaag aagtatggcc ggaagagctg ctggcgaaga aaccggaact gcgtggcaaa
     2701 acgctgtacg aagttctgta tgccaccccc gaagtgagca aattcccggt atccgaactg
     2761 gcggaagatc agctgaacga tgaatcccgc gagctgggct tctatctgca aaaagggctg
     2821 ttcgaagagt acgcatggtt tggtcgcggt cacggtcacg atctcgcgcc gttcgatgac
     2881 taccacaaag cgcgcggtct gcgctggccg gtggtgaacg gtaaagaaac acagtggcgt
     2941 tacagcgaag gtaacgaccc gtacgtgaaa gcgggcgaag gctataagtt ctacggtaaa
     3001 ccggatggca aagcggtgat cttcgcactg ccgttcgaac cggcggcgga agcaccggat
     3061 gaagagtacg acctgtggct ctctaccgga cgcgttctgg agcactggca caccggcagt
     3121 atgactcgcc gtgtgccgga actgcaccgc gccttcccgg aagcggtctt gtttattcac
     3181 ccgctggatg cgaaagcgcg cgatctgcgc cgtggcgaca aagtgaaagt ggtttctcgc
     3241 cgtggcgaag tgatctcgat tgttgaaacg cgcggtcgta accgtccgcc acagggcctg
     3301 gtgtacatgc cgttcttcga cgccgcacag ctggttaaca aactgacgct ggatgcgacc
     3361 gatccgctct cgaaagagac ggacttcaag aagtgcgcag tcaaactgga gaaggtgtaa
     3421 gccattatgt cccggtcagc gaaacctcaa aatggtcgcc gccgctttct gcgcgatgtc
     3481 gttcgcacag caggcgggct ggctgccgtg ggtgtggcgc tggggttaca acagcaaacc
     3541 gcacgcgcat ctggcgtgcg gttgcgcccg cccggagcca taaacgagaa cgcctttgcc
     3601 agtgcctgtg tgcgttgcgg tcagtgtgtt caggcttgcc cttacgacac cttaaaactg
     3661 gcgacgctgg cctctggtct gtcggcgggc acgccatatt ttgtcgcacg ggatattcct
     3721 tgcgaaatgt gtgaggacat tccgtgcgcc aaagtgtgcc caagcggtgc gctggatcgt
     3781 gagattgaat cgatcgacga cgcgcggatg gggctggcgg tactggtgga ccaggaaaac
     3841 tgtctcaact ttcaggggct gcgctgcgat gtttgttatc gcgaatgccc gaaaattgat
     3901 gaggccataa ccctggagct ggagcgcaac acgcgtaccg gtaagcacgc ccgctttctg
     3961 ccgacggttc acagcgacgc ctgtaccggt tgcggtaagt gcgaaaaagt atgcgtgctg
     4021 gaacaaccgg caatcaaggt gttaccgctg tcactggcga aaggggagtt aggtcaccat
     4081 taccgcttcg gttggctgga ggggaacaat ggcaaatcgt aaacgtgacg ccgggcgcga
     4141 ggcgctggag aaaaaaggct ggtggcgcag tcaccgttgg ctggtgttac gtcgcctttg
     4201 tcagttcttc gtgctgggga tgtttttgag cggtccatgg tttggtgtgt ggatcttgca
     4261 cggcaactac agcagtagcc tgttattcga caccgttccg ctgaccgatc cgctgatgac
     4321 gctgcaaagt cttgccagtg gtcatctgcc tgccacggtg gcgttgaccg gtgcggtgat
     4381 tatcaccgtg ctctacgccc tggcgggaaa gcgattattt tgcagctggg tctgcccgct
     4441 gaacccgatt accgacttag cgaactggtt acgcaggcgc tttgacctca atcagtctgc
     4501 gacgatccct cgccacattc gctacgtgct gttggtggtc attctgctgg gatcggcctt
     4561 aactggcacg cttatctggg aatggatcaa ccccgtatct ctgatggggc gcagcctggt
     4621 aatgggcttc ggcagcggcg cgctgcttat tctcgcgctg tttttatttg atctactggt
     4681 cgttgaacac ggctggtgcg ggcacatttg tccggttggc gcgctgtatg gcgtgctggg
     4741 tagcaaaggt gtgattaccg ttgcggccac cgatcggcag aaatgtaacc gctgtatgga
     4801 ttgttttcat gtttgcccgg aaccgcatgt gctacgtgcc ccggtgctgg atgaacaaag
     4861 cccggtgcag gtcaccagcc gcgattgcat gacgtgcggt cgctgcgtgg atgtctgttc
     4921 tgaggatgta tttacaataa ctacacgatg gagttcggga gcgaaatcat gaaaagccat
     4981 gacctgaaga aagcgctgtg tcaatggacg gcgatgctgg ccctggtggt aagcggcgcg
     5041 gtttgggcgg ctaatggcgt cgattttagc cagtcgccgg aagtatccgg gacacaggaa
     5101 ggggccattc ggatgccaaa agagcaggaa cggatgccgc tgaactatgt gaatcagccg
     5161 ccaatgatcc cgcatagcgt tgaaggttat caggtaacga ccaataccaa ccgctgcttg
     5221 cagtgccacg gtgtcgaaag ttatcgcacc actggcgcgc cgcgtatcag tcctactcac
     5281 tttatggaca gcgacggcaa agtgggcgcg gaagtggctc cgcgtcgtta tttctgtctg
     5341 caatgtcacg taccgcaggc cgataccgcg ccaatcgtgg ggaatacctt taccccatca
     5401 aaaggttacg ggaaataaga ggttattatg ggaaattctg accgtaagcc tggtctgatt
     5461 aagcgcctgt ggaaatggtg gcgtaccccc agccgtctgg cgctggggac gctgctgttg
     5521 atcggttttg ttggcggcat cgtcttctgg ggtggcttta acaccgggat ggaaaaagcc
     5581 aataccgaag agttctgcat tagctgccac gaaatgcgca acacggtgta tcaggaatac
     5641 atggattccg tgcactacaa caaccgtagc ggcgtccgtg cgacctgtcc ggattgtcac
     5701 gttccgcacg agtttgtgcc gaagatgata cgcaagctca aagcaagtaa agagctgtat
     5761 ggtaaaattt ttggcgttat tgacacgccg cagaaatttg aagctcatcg tctgacgatg
     5821 gcacagaatg agtggcggcg catgaaggac aataactcgc aggagtgccg taactgtcac
     5881 aacttcgagt atatggatac aaccgcccag aaatcggttg ccgcgaagat gcatgaccag
     5941 gcggtgaaag atgggcaaac ctgtattgat tgccataaag ggatagcgca caagctgccc
     6001 gatatgcgtg aagtcgagcc aggtttttaa cagggttatt gcgtgggtat gcttgaagcc
     6061 agagagttac tttgtgagcg ggatgaacga accttattta gtggcttgtc atttacgctg
     6121 aacgcaggag agtgggtaca aatcaccggt agcaacggtg cggggaagac aacgcttctc
     6181 cgtttgctga cggggttgtc tcgccctgac gcaggcgagg ttctctggca agggcagccc
     6241 ttgcatcagg tacgcgacag ctaccatcaa aacctgttat ggataggcca tcagccgggg
     6301 atcaaaaccc ggctgacggc gttagaaaat ctgcactttt atcatcgcga tggcgatacc
     6361 gcacaatgtc tggaagccct ggcgcaggcc gggcttgccg gattcgaaga tattcctgta
     6421 aatcagctct cggccgggca acaacgccgc gtcgctttag cgcgtctgtg gctgacccgt
     6481 gccacgttat ggatcctcga cgagcctttt accgcgattg acgttaacgg cgtcgatcgt
     6541 ctgacccagc gtatggcgca gcatacggag caggggggga ttgtgattct gactacccac
     6601 cagccgctca acgttgctga aagtaaaatt cgccgcattt cactgacgca aacgagggcc
     6661 gcatgatgtt ctggcgcatt ttccgtcttg agctgcgtgt agcgtttcgc catagcgccg
     6721 aaatcgccaa cccgctgtgg ttcttcctga ttgtaattac cctttttccg ctcagtatcg
     6781 gtccggagcc gcaactgctg gcgcgtattg caccgggcat tatctgggtt gctgcgctgc
     6841 tttcatcctt gctggcgctg gaacgactgt tccgtgacga tttgcaggac ggcagtcttg
     6901 aacaattgat gttgttgccg ttacccttgc ccgccgttgt gctggcgaag gtgatggcgc
     6961 actggatggt aaccggtctg ccgttactca tcctttcgcc actggtagca atgctactgg
     7021 gaatggatgt ttatggctgg caagtgatgg cgctgacgct gctgctggga acgcctacgc
     7081 ttggctttct cggtgcaccg ggcgtggcgc tgacagtggg acttaagcgc ggtggtgtgc
     7141 tgctcagcat actggtgtta ccgctgacta tcccattact catcttcgcc accgccgcga
     7201 tggacgcggc ttccatgcat ttgcccgttg acgggtatct ggcaatttta ggcgcgttgc
     7261 tggcaggcac cgcgacatta agtccttttg cgacggcggc agcgttacga atcagcattc
     7321 aataacgctg gattactgat ttttgttcga gtctggtatc gaaactatgt ggaaaacact
     7381 gcatcaactg gcgatcccac cacggctgta tcaaatctgt ggctggttta taccgtggct
     7441 ggcaattgcc agtgtggtcg tgcttaccgt cggctggatc tggggattcg gctttgctcc
     7501 ggctgattat cagcagggaa atagctaccg cattatctac ctgcatgtgc ctgcggcgat
     7561 ctggtcgatg ggcatttatg catcaatggc agtggcagcg tttattggcc ttgtctggca
     7621 gatgaaaatg gccaacctgg cggtggcggc gatggccccc attggtgcag tgtttacctt
     7681 tattgccctg gttaccggct ctgcatgggg aaaaccgatg tggggcacct ggtgggtatg
     7741 ggatgcacgt ctgacttctg aactggtgct gctgtttttg tatgtgggtg tgatcgccct
     7801 gtggcacgcc ttcgacgacc gccgtctggc gggccgtgcg gcaggtatcc tggtgctgat
     7861 tggcgtggtg aatctgccga ttattcatta ctccgtggag tggtggaaca ccctgcatca
     7921 gggatcaacg cgaatgcagc aaagtatcga tccggcgatg cgttcgccgc tgcgctggtc
     7981 gatttttggc ttcctgctgc tgtctgccac gctgacgctg atgcggatgc gtaatttgat
     8041 tttgctgatg gaaaaacgcc gtccgtgggt gagtgaactg atactgaaaa gaggccgtaa
     8101 atgacccctg catttgcttc ctggaatgaa tttttcgcaa tgggcggtta cgcctttttt
     8161 gtctggctgg cggtggtgat gaccgttatt ccgctggtgg ttttggtcgt gcactcggtg
     8221 atgcagcatc gcgcaattct gcgtggtgtg gcgcaacagc gggcgcgtga ggcgcgttta
     8281 cgtgctgcgc aacagcagga ggctgcatga atattcgccg taaaaaccgc ttgtggatcg
     8341 cctgtgccgt gttggcaggg ctggcgctga ctatcggtct ggtgctatat gcgctgcgct
     8401 cgaatatcga tctcttttat acgccggggg agattctcta cggcaagcgt gagactcagc
     8461 agatgccgga agtcggtcag cgtctgcgcg ttggcgggat ggtgatgccg gggagtgtgc
     8521 agcgcgatcc caattcgcta aaagtaactt tcaccattta cgacgctgaa ggttcagtag
     8581 atgtctctta cgaaggcatt ttgccggatc tgttccgtga agggcagggc gttgtggtgc
     8641 agggcgaact ggaaaaaggc aatcatatcc tcgcaaaaga agtgctggcg aaacacgacg
     8701 aaaactacac gccgccagaa gttgagaaag cgatggaagc caatcaccgt cgcccggcga
     8761 gtgtttataa ggacccagca tcatgatgcc ggaaattggt aacgggctgc tgtgtctggc
     8821 gctaggaatt gcgctgctgc tgtccgtgta tccgctatgg ggcgtagcgc gcggagatgc
     8881 gcgcatgatg gcgtcttccc gcttgtttgc ctggctgctg tttatgtctg tggctggcgc
     8941 atttctggta ctggtcaatg ccttcgtggt caacgacttc accgtcacct atgttgccag
     9001 caactccaat acccagcttc cggtgtggta tcgcgtggcg gctacctggg gcgcgcatga
     9061 aggctcgctc ctgctgtggg tgctgctgat gagcggctgg acctttgcgg tagcgatttt
     9121 tagtcagcgt attccgctgg atattgtggc ccgcgtactg gcgataatgg ggatggtcag
     9181 cgtcggcttt ttgctgttca ttctctttac ctctaacccg ttctcacgca cgttgccgaa
     9241 cttcccgatt gaagggcgcg atcttaaccc gctgttacag gatccggggc tgatcttcca
     9301 tccgcctctg ctctatatgg ggtacgtggg gttctcggtg gcgtttgctt ttgccattgc
     9361 ttctttgttg agcgggcgtc tggacagcac ttatgcgcgt tttactcgtc cgtggacgct
     9421 ggcggcgtgg atcttcttga cgctcggcat tgtgctcggt tccgcatggg cctattacga
     9481 actcggctgg ggcggctggt ggttctggga tccggtagaa aacgcctcgt ttatgccgtg
     9541 gctggtgggg actgcgctga tgcactcact ggcggtcact gaacaacgcg ccagcttcaa
     9601 agcgtggaca ttactgctgg caatcagtgc cttctcgttg tgtctgctgg ggactttcct
     9661 ggtgcgttcc ggcgtgctgg tatcggtaca cgcgtttgcc tctgatccgg cacgcggtat
     9721 gtttatcctc gcctttatgg tactggtgat tggtggttcg ctgctgctgt ttgccgcgcg
     9781 tggacacaaa gttcgctcac gcgtaaacaa tgcgctgtgg tcgcgggaat ctctgctgtt
     9841 agcgaacaac gttttgctgg tcgccgcgat gctggtggta ttactgggga cgctgctgcc
     9901 gctggtgcac aagcaactgg gactgggcag tatttcgatt ggcgaaccgt tcttcaacac
     9961 catgtttacc tggctgatgg tgccgtttgc gctgctgctt ggtgtcggtc ctctggtgcg
    10021 ctgggggcgc gatcgcccac gtaaaatccg caatttattg attatcgcct tcatctcaac
    10081 gctggtgctg tcgctgctgc tgccgtggct gttcgaaagc aaagttgtgg cgatgacggt
    10141 gctcggcctg gcaatggcct gctggattgc tgtgctggca attgcggaag ctgcgctgcg
    10201 tatttcacgt ggcacgaaaa ccaccttcag ttattgggga atggtggcgg ctcacctggg
    10261 gctggcagtg acaattgttg gtattgcctt tagccagaac tatagcgttg agcgtgatgt
    10321 gcgcatgaag tccggcgata gcgtcgatat tcatgaatat cgcttcacct tccgtgatgt
    10381 caaagaggtg actggcccga actggcgtgg cggtgtggcg actatcggcg taacgcgcga
    10441 tggcaagccg gaaacggtgc tgtatgcgga aaaacgttat tacaacactg ccgggtcgat
    10501 gatgaccgaa gcggcaattg acggcggcat cacgcgtgac ctgtacgccg cgctcggtga
    10561 agagctggaa aacggcgcgt gggccgtgcg tctttactac aaaccatttg ttcgctggat
    10621 ttgggcgggc gggctgatga tggcgttggg cggactgctg tgtctgtttg atcctcgcta
    10681 tcgtaagcgc gtgagtccgc aaaaaactgc gccggaggcc gtatgaagcg caaagtattg
    10741 ttaattccgt tgattatctt cctggcgatt gccgcggcgc tgctgtggca gctggcgcgt
    10801 aatgccgaag gggatgatcc gaccaatctg gaatcggcgc tcattggcaa gcctgtgccg
    10861 aagtttcgtc tcgaatcact ggacaatccg gggcagtttt atcaggcgga tgtgctgact
    10921 cagggcaaac cagtgctgct taacgtctgg gcgacctggt gtccgacctg ccgtgcggaa
    10981 catcaatatc tgaatcagct ttcagcgcag ggcattcgcg tggtcggcat gaactataaa
    11041 gacgatcgcc agaaggcaat cagctggctg aaagagctgg gcaatcctta cgcgctaagc
    11101 ctgtttgatg gcgacggcat gttagggctg gatctcggtg tctatggcgc gccagaaacc
    11161 ttccttattg acggcaacgg tatcattcgc tatcgccatg cgggcgatct caatcctcgc
    11221 gtctgggaag aagagatcaa gccgctgtgg gagaaataca gtaaggaggc cgcacaatga
    11281 ggtttttatt gggcgtgctg atgctgatga tctccggctc agcactggcg accatcgacg
    11341 tgttgcagtt taaagatgaa gcacaggaac aacagttccg tcagctcact gaagaactgc
    11401 gctgcccgaa atgccagaac aacagcattg ccgattccaa ctcgatgatt gccaccgacc
    11461 tgcgtcagaa agtgtatgaa ctgatgcagg aaggtaaaag taaaaaagag attgtcgatt
    11521 atatggtggc gcgttacggc aacttcgtca cttacgatcc gccgttaacg ccgctgaccg
    11581 tgctgctgtg ggtgcttccg gtagtggcta ttggcattgg cggttgggtc atatacgccc
    11641 gctcgcggcg tcgggtacgc gtagtgccgg aagcgtttcc tgaacaaagc gtgcaggaag
    11701 gtaagcgtgc cggatatatt gtttatctgc cgggtattgt ggtggcgtta attgtggctg
    11761 gcgtcagcta ctaccagact ggcaattatc agcaggtgaa aatctggcag caggccacgg
    11821 cacaggctcc ggcgttgctg gacagggcgc tggatccgaa agccgatccg ctcaacgaag
    11881 aagagatgtc gcgtcttgcg ctggggatgc gtactcaact gcaaaaaaat ccgggagata
    11941 tagaaggctg gattatgttg ggccgcgttg gcatggcgct gggtaacgct agtatcgcca
    12001 ccgatgcata cgctactgcg tatcgcctcg atccgaaaaa cagcgatgcc gcactgggtt
    12061 atgctgaagc gttgacacgt tcatctgatc ccaacgacaa ccgcctcggt ggtgaactgc
    12121 tgcgccagtt ggtgagaagc gaccacagca atatccgtgt gttaagcatg tatgcgttta
    12181 atgcctttga gcagcagcga tttggcgaag ccgttgccgc gtgggagatg atgttgaaac
    12241 tcttacctgc caacgatact cgccgtgcgg tgattgaacg tagtatcgcg caggcgatgc
    12301 aacatttgtc gccgcaggag agtaaataaa aagaggcccg atggtgatgc catcgggcta
    12361 tttttattgt gccccgcgtt gttgcaggaa cagaatggtg gctgccacgc gtgagcggac
    12421 attgagtttg cgcagcagat tgcgaatatg tacttttact gtctgctctg aaatattcaa
    12481 caccgaggca atctgtttat ttgacagccc ctgtgccagc tcgtgcagaa catccagctc
    12541 gcgctccgtc agcacgctga agggatcttc ttccgcgcca aacatttcac gttcacgtaa
    12601 gtactgattg acgcgttcgc taaagacttt gctgcctttc gctccagcac gaattgcttc
    12661 cagcaatact tccgggtcgc tgtctttcaa cagataaccg tctgcgcctg cgtctatcag
    12721 tgcaaagaca tcgctggagg catctgatac ggtcaggata ataatttgcg cggtaacgcc
    12781 atccctgcgc aaggcattga gggtatccag gccactcata cctttcatat tgagatccag
    12841 caagatcacg tcgatatcca gtctattcgc cagatcgatc gcgctcgcgc cgtcgcccgc
    12901 ttcggcgacc acttcaaagc caggatcaag ctccagtaac tgacgaacac cgcgtcgcat
    12961 aagtggatga tcatccacaa tcatcacctg aaaaggcgtt gcttcaggca tagtagtctc
    13021 ctgaggtttt attagaatca ttatgtttga gcgtagcgtt ggtaacgcta ccaggcgcgt
    13081 gaatgttacc ccattttatg tatggacatg tagtgaaaac tacaaccttg tcgttcgctt
    13141 tggcatcatt ttcattataa agacggaatt ttgcatcagc ttctcatcaa gaaaaacaac
    13201 taaaaaatca atcacaatac ttgtgcattt ctttaaacag tttttaaaaa ctgactccgg
    13261 gtatggagct atgggtattt tccgtaccca atgcttttaa cagcaattaa tttcatagga
    13321 tgaaagctca attcatcaat ctggttctgt ttctctttgt cgttccgcaa tatctgttct
    13381 ggtggctaca gcgttatatt cacccatagc attggcatca acagttgagt atggtgagac
    13441 tgttgatggt gttgtcctgg aaaaagatat ccagctggtt tatgggaccg ccaataatac
    13501 gaaaatcaat cctggcggag aacagcatat aaaagaattt ggtgtaagta gtaatactga
    13561 aattaacgga gggtatcagt acattgaaat gaatggtacc gcagaatact cagtattaaa
    13621 tgacggttat caaattgttc aaatgggtgg cgcggcaaac cagactaccc tcaataatgg
    13681 tgtgctacag gtttatggcg cagcgaatga tcccacgatt aaaggcgggc gcttaatcgt
    13741 tgaaaaagat gggattaccg tccttgccgc tatcgaaaag ggaggattac tggaggttaa
    13801 agagggggga ttagcgattg cggtagatca gaaagcaggc ggtgctatta aagcaagcat
    13861 gcgggtcatg gaggtattcg gaacaaaccg tctcggtcag ttcgaaatca agaatggtat
    13921 tgctaacaat atgctgttgg aaaacggcgg aagtttgcga gttgaagaaa atgacttcgc
    13981 ttataatact actgtagata gtggcggctt actggaggtt atggatggcg ggactgcaac
    14041 tggcgttgat aaaaaagcag gcggaaaatt aattgtctca acgaatgcgc tggaagtgag
    14101 tggtacaaac agtaaaggcc aatttagtat aaaagatggt gtgtcaaaaa attatgaact
    14161 ggatgatggt tccgggctca ttgttatgga ggacacgcag gccattgata ctatccttga
    14221 tgagcatgcc actatgcaat cgctgggaaa ggatactggt acgaaagtgc aggcaaatgc
    14281 ggtatatgat ctcggtcgat cagatcagaa tggaagtatc acgtattcct caaaagccat
    14341 ctctgaaaat atggttatca acaatggccg cgctaacgtc tgggctggca caatggttaa
    14401 cgtttcagtc agagggaatg atggcattct tgaggtcatg aagccgcaaa taaattatgc
    14461 acccgcaatg ttggtgggta aggtagtggt ttctgagggc gcttctttta gaacgcatgg
    14521 tgccgtggat accagcaaag cggacgtttc gctcgaaaat agcgtatgga ccatcattgc
    14581 cgatatcact acgacgaacc aaaacaccct cctcaactta gccaaccttg cgatgtctga
    14641 cgcaaatgtg attatgatgg atgagccagt gactcgttca tcagtgacgg caagtgcgga
    14701 aaatttcatt acgttgacca ccaataccct gtcgggaaac ggcaattttt atatgcgtac
    14761 cgatatggct aatcatcaga gcgatcagct caacgtcacc ggtcaggcaa caggtgattt
    14821 caaaatattc gtgacggaca ccggtgccag cccggcagca ggagatagcc ttacactggt
    14881 aacaacgggc ggcggtgatg ctgcatttac gttgggcaat gccggaggcg ttgttgatat
    14941 cggtacgtat gaatatacct tgctggataa tggcaaccat agctggagtt tggcagagaa
    15001 tcgcgcgcaa attacccctt caaccactga tgtgctgaat atggcggcag cacaaccgct
    15061 ggtatttgat gcagaactgg acaccgtgcg tgagcgtctt ggtagcgtaa aaggcgttaa
    15121 ttacgatacg gcgatgtgga gttcggcaat taacacccgc aacaacgtga ccactgatgc
    15181 gggcgctggc tttgagcaaa cattgaccgg gctgacgctc ggtatcgata gccgtttctc
    15241 ccgtgaagaa accagcacaa ttcgcggctt gttctttggt tactctcatt ctgatattgg
    15301 ttttgatcgc ggcggcaaag gcaatgtcga tagctatacc ctgggggctt atgccggttg
    15361 ggagcatcag aacggtgcct atgttgatgg agtggtgaaa gttgaccgtt ttgccaacac
    15421 catccattgc aagatgagta atggggcaac agcgtttggc gattacaata gtaacggcgc
    15481 gggtgctcat gtcgagagcg ggttccgttg ggttgacgga ttgtggagtg ttagacccta
    15541 tctggccttt accggcttta ccacagatgg tcaggactac acgttatcaa acggcatgcg
    15601 cgcggatgtg ggaaataccc ggatattacg cgctgaagcg ggaacggcgg taagctatca
    15661 catggacctg caaaacggta cgacgctgga accctgactg aaagccgccg tgcgtcagga
    15721 atacgccgat tctaaccagg tgaaagttaa tgacgatggc aaatttaata atgatgtggc
    15781 tggaacccgt ggcgtttatc aggctgggat aaggtcatcg tttaccccga cgttaagcgg
    15841 tcatttgtca gtcagctatg gcaatggcgc aggggtagaa tcgccgtgga atactcaggc
    15901 gggtgtggtc tggacgttct gataacaaaa aataaacagg ctgtgatgtg tcacggtctg
    15961 tttatcgaat taattgcaga tataaaaaaa accaaccgta aaggttggtt ttttcttggg
    16021 atttttggtc ggcacgagag gatttgaacc tccgaccccc gacaccccat gacggtgcgc
    16081 taccaggctg cgctacgtgc cgactcgtgg ctgctaatac taccgttttc cacaccgatt
    16141 gcaagtaaga tattttgcta actgatttat aattaatcag ttagcgataa aacgcttctc
    16201 gtctgtcagc acttgcaata acaaacttaa ctgtggtttt tcatctttca ctctttcacc
    16261 acgtaagttg taagtgcggt atttaccgtt attgttcagc accagcgtct ttttcggggt
    16321 ggtaattgcc agcgtatcgt tatccgctgc agtaacccag taatgacggc gttgagggtt
    16381 gaacaaatcc tgaccttgcg aatattcgct ggcaggtgtg ctgacatgta gcaggcgttg
    16441 catcagcgtc gtcatcagat cggtatgatc agtcagagca ttaatacgct gcgccggcgt
    16501 gcctggccag tgaatcacta atggcacctg cagatgaccg tgggaccagt caaaggtttc
    16561 ttcgtcttcg ctcagcggaa taccccgacc ggcagtgata atcaccaccg tattgtccag
    16621 tttgccagaa tcacgcagtg cattgagcac gcggttgatc tggtcatcga cattgcctgc
    16681 cgcccggcta tatttccgtg caaatgcctg ctgattgctg tcgtcaatgt tagtgccatt
    16741 gaaagagacc cacgagaacc agcggttatc ttcttgtgcg tagcggccca gccagttgat
    16801 ccactgcgtg gcggtctgct cgtcggattg ggtgcgtacg ctcggcatcg agaaatctga
    16861 caacaatgcc tggcgataca gcgggctggt aaagccatct gatgagaata atcccagctg
    16921 atagccttgc tgattaagcg cagtaattaa cgccgcaggg gtacgggtcg acagaatgcc
    16981 gtccatatag ctcggcgaga tgccatagaa caggccaaag atgccgttgt ctgtagtgtt
    17041 gccggagctc atatggcgcg tgaacgaaat attttgctca gcaaaacctg ccagcgcagg
    17101 catctgcttc tcgaagcgtg agtagttcag gccatcgaca gtaatcaaca gcacattctg
    17161 cccggtgccc atatcgcgat agcgcagttc gcttaacgga tactgaacgg aaacggcgtc
    17221 tggattacct tgctcaataa gacggcgttg atactcctgc gcatcaagca gaccatgctt
    17281 ctcaagaaaa cgtcgcgccg tcatcgggta cgaaagcggc aggttagcgc gctgcatggt
    17341 gatcgggcga tagaagttgg catcggccca gatatacacc acatgcgagg cgataaaggc
    17401 gataaataag aatgcggcca gcgggcgcgc gaagcgccga cgacgcgtca ggctgcgcag
    17461 cttttgccag ctccacgtcg caaacaccag ttcaagcaat aaaataaccg gcacgctgat
    17521 gaacatcagc tgccagtcgc gcgccatctc attttcgtct gggttgataa ccagttgcca
    17581 gacgatggga ttaagatgga gatggaaacg agtaaagact tcgctatcga tcagtaatag
    17641 cgtcattccc gccgttgcca gaatgacgga caaaaacctc atcagcctct gggagccgac
    17701 gataaaggtc agcgggaaga ggatcagcaa gtaggtggcg aacaccagga agctgaaatg
    17761 gccgataatg cttacgtagg aataaatgcg accagcaagc gttgtcggcc agtcggcgat
    17821 aaacaggtaa cggctgccaa tgacgagcga aagcagaata ttgaacagtg caaaccagtg
    17881 cccccaactg accatctggg agactttttc acggtagcgc tgacgatgag ttaccataac
    17941 ctgttatctg tttcccttag tgcgctttgt cttcgttgat agaggactgt aaggcgcggg
    18001 caaaagagtt ggcaattgcc tggcgttggg ccggggcaat gctggtattg ataaggttag
    18061 tgaccatgtt gcctaacacc atcagggaaa ggtcggtcgg agccttatgt ttttccagta
    18121 cgttgagcag ctctgcgagc aattgttcaa cctgttcatc actgtagcgg gaaatttgtg
    18181 gcataaatcg aaatcagtta gttgatgaaa gggcaacata ttaccgtagc aacggttttt
    18241 tttctgcttt tttatgcgtt aaatcattct cgttacgctg ccttctgtaa aatgaccgcc
    18301 atcttttatt gcgtcgctta accggcggtg gttgaatacc gcccggtctt aaaggagagt
    18361 ttatcatgag tctggatatc aaccagattg ctctgcacca gcttatcaag cgtgatgagc
    18421 agaaccttga gctggtgttg cgcgattcat tgctggaacc gacagaaacc gtcgttgaga
    18481 tggtggcaga actgcatcgg gtctatagcg ccaaaaataa agcttacggc ttgtttagcg
    18541 aagagagtga actggcgcag acgctgcgct tacagcgtca gggcgaagag gactttctgg
    18601 catttagccg cgcggcaacc gggcgcctgc gtgacgaatt agcgaaatat ccttttgctg
    18661 acggtggatt cgtgctgttt tgccactacc gttatctggc ggtggagtat ctgctggtgg
    18721 cagttctgag caacctgagc agtatgcgcg ttaacgaaaa tctcgacatc aatccaaccc
    18781 attatcttga tatcaatcat gcggatattg ttgcgcgtat cgatttaacc gaatgggaaa
    18841 ccaatccaga gtccacccgc tatctcactt tcctgaaagg gcgagtaggg cgcaaagtgg
    18901 cggatttctt tatggatttc ctcggggcca gcgaagggtt aaacgcgaaa gcgcaaaacc
    18961 gtgggctgtt gcaagcggtt gatgatttca ctgccgaagc acaactggat aaagcagagc
    19021 gacagaacgt gcgccagcag gtttatagct actgtaatga gcagctgcag gcaggcgaag
    19081 agatcgaact ggagtcgctg tcgaaagagc tggcaggcgt cagcgaagtg agtttcacgg
    19141 agtttgcggc tgaaaaaggc tacgaactgg aagagagctt cccggcagac cgtagcacac
    19201 tgcgtcagtt gacgaagttt gccggtagcg gcggtggctt gactattaac tttgatgcga
    19261 tgctgttagg tgaacgcatc ttctgggatc cggcaaccga taccctgacc attaaaggca
    19321 cgccgccgaa tttgcgtgac caattgcagc gccggacgtc tggcggcaat taatcggttt
    19381 tgtgttctga atgccagatg cggcgtgaac gccttatctg gcctgcaaaa aatagcaata
    19441 ccgtaatcag gcataatctg cagatacaaa aaaccccgcc ggagcggggt tttttacaac
    19501 ttattcagca attaagcgcg aacgaagtcg atgtgctgca gcttcggttt gtacgggtga
    19561 cgctgtacgt cctgagcttt aactttgatt tctttaccgt caacaacgat ggtcagaact
    19621 tcgctgtaga attcagcttt agcttgcatg ttcatgactt tgtcgtgatc cagctcgata
    19681 gccagcggcg cttctttgcc accgtagatg attgccggga acttgttagc ggcacgcagg
    19741 cggcggctcg cacccttacc ctgctcttta cgtacttctg cgttgatagt aaacatttct
    19801 ttctctctta aattaatcct gctacaggcg acccagcaac aggtaagtga tctgctttgc
    19861 gtatgcaaaa gcgggcgaga ttctatactc aatcgcttta tacatcaatc aaaactacca
    19921 ttaaccgcgt aattcgtgcg cccgacgaaa acgaccttcg taatcgaaca ctttttcacg
    19981 cacttgccag tactgacctt tcatgcgggc aacgacaaaa tccgggtgtc gcaataaggc
    20041 ttgctgggcg aggatatcgg cggcggtgat ccagcgcaga gggatgcccg gtgtgcgcgt
    20101 atgcgggcgg ataaaaagct gctcgaaggc agttcgctgg gcgggcgttt gcagacggaa
    20161 acgctcactc acatccgcgc cgtcttcatc gtaataggtg attttcaacc attcgccttt
    20221 ctcgtcatgc ccatgctgta aagacatgcc gctacagcgt aataccagcg cgtctttcag
    20281 tcgtagcgcc gcttttaaca tatcgtctgg atcaaccagt acggtgtcac attcgcggca
    20341 gcggcgagcg gcaatatcgt tttccgcgtt acattgcggg caatttttaa aacggaaacg
    20401 gaagtcacat tgttcgcgat gaccgtcgtc atcttcaaac catccctgac agcgacgacc
    20461 aaagtgttca atcaatgtcc cgtcggcggt cgttttcccc caaaaggtgt tggcaaaacc
    20521 gcaggcaggg cagaaaacct gtaccggaac gttgtcactt ttgccttttg gtgtaccaac
    20581 ttccggcgcg tagagatcgt gaggattacc cgcataatca agaattaagc aatcagtctt
    20641 gcccggagcg agacgcagac cgcgcccgac aatttgttgg taaagactca ctgattcggt
    20701 agggcgcaga atggcgataa gatcgacgtg cggggcgtca aatccggtgg tcagtactgc
    20761 gacgttgacc agatagcgaa aacgctgggc tttaaaatct tcaattaaca catcgcgctc
    20821 agcgccgggg gtgtcgccag taatcagtgc agcatcttcg gcaggcagta atcccacaat
    20881 ctcttttgcg tgttcaacgg tggcggcgaa aatcattacc cctttgcgct tttcggcgaa
    20941 ctccatgatc tggctgatga tatgtggcgt aatgcgttgc tgttttttca gctcacggtt
    21001 gagatcagct tcgctgaaca gcccgttact ctgtgcctgc aagcggctga aatcgtattg
    21061 cactactggc atatccagtc gttctggcgg cgtcagatag ccgtgtttaa tcatataacg
    21121 cagcggcagc tcataaatgc agtcacggaa aagggctttc tcatcgccgc gtaccatgcc
    21181 gtgataatgg aactggtaga tccagccttt gcccaatcga aaaggcgtgg cagtcagccc
    21241 cagcaggcgt aagtggggat tcactttagt caggtgagtg aggatttgct gatactggct
    21301 ctcttcatcg tcaccaatac ggtggcattc atcgacaatc aacagcgaaa attcaccctg
    21361 aaaggcatca agattacgag cgaccgactg cacgctgcca aataccactt taccgtggct
    21421 ctctttgcgc tttaacccag cggcaaaaat atcggcttcc agccccagcg cctgatactt
    21481 tgcatggttt tgcgccacca gttctttgac gtgtgccagc accagaacac gaccacgcgc
    21541 cagccgtgcc agttcggcaa tcaccaggct tttacctgcg ccggtgggca gcacgataac
    21601 ggcaggggtt ttatgacgac gaaaatggtt gagcgtggca tccacggctt cttgctgata
    21661 tgggcggagt gtaaaaatca tggtctcgcg taggtagatt attctgctaa tagtatgcca
    21721 tggatctttt tcccttgagg gatgttatta gaccgctata ctgggcagac aacagttcag
    21781 attttcgggt caatgcccga cctccgtttt tacaggcaaa atcacacaca tgcgacttga
    21841 taaatttatc gcacagcaac tcggcgttag ccgtgctatt gccgggcgtg aaatccgcgg
    21901 caatcgtgtc accgtcgatg gcgaaattgt tcgtaatgca gcgttcaaac tgcttcctga
    21961 acatgatgtc gcttacgatg gcaacccgct ggcgcagcaa catggtccac gttacttcat
    22021 gctcaataag cctcagggct atgtttgctc cacggacgac cctgatcacc caacggtgct
    22081 ctattttctg gatgaaccgg tagcctggaa actgcacgcc gcaggacgtc tggatatcga
    22141 taccaccggt ctggtgctga tgactgatga tggtcagtgg tcgcaccgca ttacttctcc
    22201 gcgccatcat tgcgagaaga cctatctggt gacactggaa tcacctgtag ctgacgatac
    22261 ggcagagcaa tttgctaaag gcgtgcagct gcataacgaa aaagatctca ctaagcctgc
    22321 ggtgctggaa gtgattaccc caacgcaggt tcgtctgacc atcagcgaag ggcgttatca
    22381 tcaggtgaaa cgcatgttcg ccgccgtggg taaccacgtg gttgagctgc atcgtgaacg
    22441 tattggcggt attacgctgg atgctgattt agcccccggt gaatatcgtc cgttaactga
    22501 agaagaaatt gccagcgtcg tctaactttt ccatgacttt caggagcccg ttgtgaccac
    22561 ccgacagcat tcgtcgtttg ctattgtttt tatccttggc ctgctggcca tgttgatgcc
    22621 gctgtcgatt gatatgtatc tgcccgcgct accggtaatt tcagcgcagt ttggcgtacc
    22681 tgcgggcagt acgcagatga ccctcagtac ttatattctg ggctttgcgt tggggcagtt
    22741 aatctacggg ccgatggcag acagcttcgg gcgtaagccg gtggtgctcg gcggtacgct
    22801 ggtgtttgcc gccgccgcgg tggcgtgtgc gttggcaaac accatcgatc agctgattgt
    22861 gatgcgtttc ttccacgggc tggctgcggc tgcggccagc gtggtcatta acgccctgat
    22921 gcgcgatatt tacccgaaag aagagttctc gcggatgatg tcgtttgtca tgctggtgac
    22981 cactattgca ccgctgatgg caccgattgt cggcggttgg gtgctggtgt ggctgagctg
    23041 gcattacatc ttctggatcc tggcattagc ggcgattctg gcttcggcaa tgattttctt
    23101 cctgattaaa gaaaccttac caccggagcg tcgccagcct tttcacattc gtaccactat
    23161 tggtaacttt gcggcgctgt tccgtcataa gcgtgtcctg agttatatgc ttgccagtgg
    23221 tttcagcttt gccgggatgt tctcgttctt aagcgccgga ccgtttgtct acattgaaat
    23281 taaccacgtc gcgccggaaa actttggtta ttactttgca ctaaacattg ttttcctgtt
    23341 cgtgatgacc atttttaaca gccgcttcgt ccgccgcatt ggcgcgttaa atatgttccg
    23401 ctcggggttg tggatacagt ttattatggc ggcgtggatg gtcatcagtg cgctgctggg
    23461 gctgggattc tggtcgctgg tggttggcgt tgcggcgttt gtgggctgcg tgtcgatggt
    23521 gtcatccaat gcgatggcgg tcattcttga tgagtttccc catatggcgg gaacggcatc
    23581 ttcgctggca ggaaccttcc gttttggcat aggggcaatt gttggcgcat tgctttctct
    23641 tgcgaccttt aactctgcat ggccgatgat ttggtcaatt gcattctgcg caaccagctc
    23701 cattctcttc tgtctgtacg ccagtcggcc gaaaaaacgg tgatctattg cacaacgagg
    23761 aaggtaaaag gcttcctttg ttgatgcatg tcaaccacaa atctatcatt tccccgatat
    23821 atgtttattt tatgtaaaat caatttatgt aaaaagtcac atcattgtag ttaaaaaggt
    23881 tgagttagat cgcagaaacg ggtacatata gccccgcaaa cgtgaccacg cccgcagata
    23941 ttacttaaat cagagccata gaggccacgc aggcgaggca tcaatcttta cgatctgtat
    24001 aaagacggat tgttgatgat gtgttaaaat tgatgtaaac aaattgtgaa gtgaatgtac
    24061 ttccggggaa aataagtgac ttcattacaa ctctcaatcg tccatcgact gccgcagaac
    24121 tatcgctggt ctgctggttt cgcaggttcg aaggttgaac cgattccgca aaatggaccg
    24181 tgcggtgaca acagcctggt ggcgcttaaa ttgcttagcc cggatggtga taatgcatgg
    24241 tcggtgatgt ataaactaag ccaggcgtta agcgacatcg aagttccatg ttcggtgctg
    24301 gagtgtgaag gtgagccgtg cctgtttgta aatcgccagg acgagtttgc tgcaacatgc
    24361 cgattgaaaa attttggtgt ggcaattgcc gaaccctttt caaactacaa tcctttttaa
    24421 gcgtcagctc aacgccagta gctgacgcgt atactcctgc tgcggtgtgg caaacacgcg
    24481 cgcgcacggt ccttgttcca ctacttcccc ttgtcgcagt acgataacct gatgacataa
    24541 cgcgcggaca acgtgcaaat cgtggctgat aaacaaatag gccagttgat gcttttgttg
    24601 caatgatttc aatagcgtca atatttgcgc ctgtaccgtt ttgtcgagtg atgatgtcgg
    24661 ttcatcaagt atgatcagcg agggcttaag aattaatgcc ctggcaatcg caatacgttg
    24721 tcgctgacca ccagagaact ctgccggata acggtggcgt gtttcaggat ctaatcccac
    24781 ttcatgcatc acggctatca cttgttgttc gcgttgtgcg gcagaaagcg tcggctggtg
    24841 aacccgtaag ccttcctcaa taatctgcaa aacgttgagt cgtgggttga gcgaggagtt
    24901 tggatcctga aataccacct gaatgcgatg acgaataggt aacagctggc ggcgatttaa
    24961 attttgcagt ggctgaccgt caaagatgat gctgccctga gaattaatca gtcgcagcag
    25021 cgccagtccc gtcgtacttt tcccggaacc ggactcgccc actaaaccca gtgtttcacc
    25081 cgctcgtagc gtaaaactga tgtttttcac caccacatta tgatccacaa tgcgcttcaa
    25141 aatccctttg cgaatgggga aggcaacctg aagctgttca acatccagca acgttgaggc
    25201 aggttctggc aacggcactg gagcgcctga cggttcactg ttgagtagct tttgtgtgta
    25261 aggatgagtg ggtgatgcaa atagcgtagc ggcgtaattt tgctcgacac agcgaccgtt
    25321 ttgcattacc gccacgcggt gggccagttt tctgacaatg ctgaggttat gagtaataaa
    25381 cagcatgccc atattcagct cgccttgcag ttcgcgcaac agctgtaaaa tctgcgcctg
    25441 gacagagacg tccagtgcgg tggtcggttc atcggcaatt aataattccg gtcgcgttaa
    25501 cagcgccatc gcaatcatca cccgctggcg ttcgccgccg gagagctgat gcggataatc
    25561 tgtcagccgt tttgccgcct ggcggatacc aacgcgatca aggcagttaa gaatttcgcc
    25621 acgagccgct tcccgacgca tcccgcggtg gagtgaaagc acttcataaa gctgtttttc
    25681 cagggtatgc aatggattta acgacaccat cggttcctga aaaatcatgg cgatcttatt
    25741 accgcgtaca ccgcgcaacg tttgatcgct gacatgaagc agcgattcgc catgaaaacg
    25801 aatatcgccg gagagatatt caaccggcgg ggaagggagc aggcgtaaaa ttgacagcgc
    25861 ggtaacgctt ttgcctgaac ctgactcacc caccagcgcc agcgtttcgc cagcctcaat
    25921 ctgtagtgaa acatcattga ctactgtacg tacggtttgc tgatggcgaa aacccaccga
    25981 caaattttca atcgctaaca gagtttgcgt catgctacac cgccttatta ggatcaaatg
    26041 cgtcgcggac ggcttcacca ataaagatca gcaaagacaa taatatcgcc accgacaaga
    26101 aggcggtgat cccaagccac ggggcctgaa ggttattttt cccttgtaac agcagttcac
    26161 cgagtgacgg tgaaccgagc ggtagaccga agccgaggaa atcgagcgag gtcagggtgg
    26221 ttatcgaact acataaaata aacggtaaaa aggtgagggt cgcgaccatg gcattaggca
    26281 acatatgacg caggatgata ctgcgatcgc tgacgccaag cgcctgtgcc gcacgaatgt
    26341 agtcgaaatt acgagtacgt aaaaactccg cccgcaccac gccgaccaga ctcatccagc
    26401 caaacaagac agtaattgcc agcagccacc agaagttagg ctgtacgacg ctggaaagta
    26461 aaataatcaa aaacagcgtc ggcatccccg accatacttc aataaagcgt tgtccccaga
    26521 gatcgacttt accgccgtaa tacccttgta gcgcccccgc cagcacgccc atcacgctgg
    26581 aacagagagt cagcatcagg ccaaacagaa ccgagatccg cgtgccatag agaatgcgtg
    26641 ccagcacatc gccgccgttg gcatccgttc ccagccagtt ttgccgggag ggtggagaag
    26701 ggaagggctt atcggtagca aagttgatac tggtagcacc aaagcgaatc ggtgcccaca
    26761 gtacccagcc gttattttcc agccgttgtt tcagccacgg gtcctgataa tcagcctgac
    26821 ttgccagcgg gccgccaaaa tcgctttcgc tgtagttttt caacaacggg aaataccaac
    26881 tgccgtcata acgtaccagc aacggtttat cgttggcgat aagttcagaa cacaaactca
    26941 aaccaaacaa aacgaggaaa atccataacg accagtagcc gcgacggtta tggcgaaaac
    27001 gcgcccaacg ggcctgattg acggggctga gtcgcgacat taacgtccct caaaatctat
    27061 acgcggatcg accagcgtat agctgatatc actgacaata ttcagcagca ggccaatcag
    27121 ggtgaaaata tacaaggtac caaacattac aggataatcg cgcgagacgg tcgcttcgta
    27181 gcccagtaag ccaaggccat tgagtgaaaa catcacttca atcagcagcg agccggtaaa
    27241 aaacatgctt ataaacgtcg ccggaaaacc ggcaatcacc agcagcatgg cgttgcggaa
    27301 cacatgtttc cagagaatat ttttttcact tacccctttc gcacgcgcgg tcaccacgta
    27361 ttgcttgcgc acttcatcaa ggaatgagtt tttggtcagc atggtcagcg ccgcaaagcc
    27421 accaatcact gtcgccagca ccggcagcgt gatatgccac agataatcgg tgattttctg
    27481 ataccacggc agcgaatcaa agttagcgga aaccaggccg cgtagaggga acaggtcgaa
    27541 atagctgccg cccgcgaaga agacaatcag caggatggca aacaaaaagg ccggaatggc
    27601 gtagccgatg atgataaatg cgctactcca gacgtcaaag cggcccccat tataaacagc
    27661 tttgcgaatg cctaacggaa tcgacaccag atagataatc agcgtgctcc acaatccgag
    27721 ggtgatggaa accggcagac tgtctttaat cagcgtcagc accgaggcgc tgcgaaacag
    27781 gctatcacca aaatcaaagc ggatgtagtc ccagagcatt ttgaagtaac gttcgtggat
    27841 cggcttatcg aaaccgtagc gatgagtgat ctcagcgatc acttctgggt ctaatccgcg
    27901 tccgccacgg taattactgt cgctgatatt gccgacaccc gtttgcgcat ggctggcacg
    27961 aacaccttca ccgcctgcgc cgggtaatac tccggcatta ccaaactcaa tggcggcgat
    28021 ggcctggtcg accggaccgc caggcgcaat ttgcacgatg aaaaagttga tggtgataat
    28081 cgcccataat gttgggatca ccagcaacag acggcgaatc aggtaagcgc ccatctactc
    28141 tccctgtttg ctggcggacg gcagtttggc cgctttattg acgtcatacc accaggtatc
    28201 gataccgagg ctatagatgg ggcgcacggc cggctgggag aatttatccc accaggcgag
    28261 acggtcttcc gccatgtacc acattggcag catgtaataa ttccacgtta atacgcgatc
    28321 cagtgctcgc cccaacggca gtaatttttc tttatttccc tgcgcggcaa taatttgatt
    28381 gatcagcgag tcgataaccg gactttgcac gccgggggca ttataagtgg aattgatata
    28441 ttccgatgac caggaaatct gtaaatcgga actgggccac ggcatcgccc gccataggcg
    28501 cggcatcatg tcatagtcgc gactgcgcat gcggttagtg atttgcgagt tatccacctt
    28561 gcgaatgtcc atgttgatac ccagccgttg caggctgtgc tggaacggca atacccactg
    28621 actattgctg cttgcgggaa gcaataattc aaagctgagt ggctgacccg ttgtggcatt
    28681 aacgcgttgc tgacccttca gcacccagcc cgcttcgttg agaagtttgt cggcttttaa
    28741 caggttgtca cgatcgtagc catcgccttt ggataccggc ggctggtaga tttgtgtgaa
    28801 gacttctggc ggtagatctt ttttcattgg tgccagaagc accagctccg cggcgtcggg
    28861 gtaatttctg gccgcatatt cggtattctg aaaataactg ttcgtgcgac tccaggcgtt
    28921 gtaaaacaac gccttgttca tccattcaaa gtcaaaggcg agagtgattg cttcccggac
    28981 ccggcgatcg ctgaataccg gacgttggat attaaacgcc agccagcgcg tatcctgggc
    29041 tgattcgttc ttttgctcat ctttgatgat gtatttttta tcgaaatttt taccggtata
    29101 acgcgtggcc cagtttttag cgtcgttttc catacgcaaa tcaaaggcac cggctttaaa
    29161 cgcttcaaag gcgacattat catcgaggta ataatcgtag cgaatggtgt cgaaattcca
    29221 gcgtccacgg tttaccggta agtttgctgc ccagtaatct ttcacgcggg aatagacaat
    29281 attttgcccc attttccagg acgtaacgcg gtacggacca ctggcaagcg gaggcgtggc
    29341 gagcgggtcg ctaagtttgt gattcttcca gtacttttct ggaaataccg gcagcgaaaa
    29401 cagactcagc atatcttctt tgccgggttt agctaactca atgcgcacgg ttaacggcgc
    29461 aatggctttg acggtggtgc ctttgtagac cagacgaaat tgcggcacgc cttcggtcat
    29521 aaatttttga aaagtaaact ctacatcgcg ggcagtaatg ggcgaaccat catgaaaacg
    29581 ggcgcgcgga ttaatagcga cctccaccca ggaataatcg tcagcatagc gtgcgctttc
    29641 agcaatcagc gggtaataac tgcctggttc gtcatcggaa gtcgtaaata gcgtgtcgta
    29701 cagctgctcg gtgcgtgcgc ccgggttgcc gcgcagtgca tagcggttga aattatcgaa
    29761 ggtaccgagg gctgacaacg ttatctgccc accttttggc gcggcggggt tcacataatc
    29821 aaaatggttg aaattaaacg cgtaccgggg ttcgcccagc acggcaaagg catagctttc
    29881 cttgatagcc tgcgcctgcg caccaaaggt gaacagagcg ataaacagca gcagtatgcg
    29941 cacaatcatc tgcaacggat ttccttttac tcatcattag ccgcaatccg gcatgctttc
    30001 actatgaata ataagggacg taagcacacc ttaccactgc ggcgtatacg gtttcttcag
    30061 ccagcgaaca aaatcgtcca gcggtaacgg gcgactaatc cagtagcctt gcatgaaatt
    30121 aacgccgcgt tcgcttagcc atcgcgcttg ttccggcgtt tcgacccctt cagcaaccgt
    30181 cagcatattg aggcgtttcg ccagcgtcag caccgcgtca agtacgggtg aagtgatcgt
    30241 ttccgtaccg atggcgttga taaatccacg gtcaattttc agataatcga gcgtaaaacg
    30301 ctcaagatag ataagcgcgc tgtgcccggt gccgaagtca tcaatagcaa tttctacgcc
    30361 taccgagtgc agccaggcga agagttgtgt ggcttcttgc tctttcagca tatcgcgctc
    30421 ggtaatttcc agcacaatct ggaaatggtg tgcgggtagg gaagtgagca gtttctggat
    30481 atctgcttta aagctttcgc tgtgcagatg gtccggcgca atgttaatgc caaatttgac
    30541 gcctaccggc agcacttttt ctaattctgc ggcatcgcgg gcaattaact caaacagatg
    30601 ctgagtcagc ggcacaatca tcttttgcga ttcggcaaag ttaatgaagg catccggggg
    30661 aatttctccc gcgacaggat gccgccagcg tagcagtact tccaggcccg ttactcgcaa
    30721 agcttgtgta tccaccaccg gttgatacgc cacgtaaaat tgttcgcgct tgatggcggt
    30781 catgatttct ctgccggggc gcatacgtac gctcatcagg taatagcaga gcaggccaac
    30841 gacagttcca ctcatgccgc ccagtaaaaa tgcgtaccac acatcgttcc atgtccagtc
    30901 atctgcataa agccgaacgg tcagaggaat gcccgcaatt ttagtttcac ggactggcat
    30961 gtcggttaat tcgttaacgt tcatcaaacg tgaagaaaag gtagatagcg cagtattgcc
    31021 aataatgagg gcgacgccat cgtaatcttc ctgccgtgaa ctataaaaga gtgaaggcgt
    31081 caggttgaga ttcagagcgg caaagacgcc gctatttttc agcaaagggt tgcgatacca
    31141 gatgacgatt gcgggtttgt tcggcaccat cggcgtgccg ggtaagatcg ccatatcgac
    31201 atttttatta atgtcgagcg ccggaatcaa ttcattgagt ggaatgtcca tctcaccggt
    31261 cgcagatgaa cagaatgttt ttttatcttt caccagcaca aacgtacgga cattcatgct
    31321 aaacgccgcg cgggcggtca gttcggggtt agcctgctgg caggtatcta aggtcagcgg
    31381 ctggagccgg tcagtagtgg atttcaggtc ggcaaaatag gtatcgagat acttttgtac
    31441 gtcggtaatc agtgtgtcgt atttgacttc gtgcttatgc caggccacta aaaactgaag
    31501 gcaactcacc agtatcgcaa tcatcacgcc tgcaacaatg caggtaagca ggagcttacg
    31561 tccagaattg ggagcgcgta tgaacatgat tccgttaatt tcctgaaatt acgacaacat
    31621 ttaaaagata acaatgaccg gactatagcc tggatcaaga cgcggaaaca tcattttctc
    31681 tgatgaatga aaacaaaaaa gcactgccta agcagtgctt tcagttaact cgttaggata
    31741 gccaagggat tgcatccaaa cggtttatta gctgcggctg agaacccggc gtgcttcgtt
    31801 gtaacgcttc ttccagtacg gttcattcat gctggaaata ataacaccac tgctggtgga
    31861 agcatggaca aactgattgt tgccgatata aataccgaca tggcgtcccg ttgaaccggc
    31921 acggaacaga actaaatcac ccgtacgcaa attactgcgg gaaacagatt tacccatttc
    31981 ctgctgttcg taagtcgaac gcggaagttc taagccaaat tgctcacgga atgtacgctg
    32041 tacgaaacca gaacaatcga tacctttttt agtgctgccg cccagacgat aacgtacgcc
    32101 tttccagtca gcatactgat ccataattcg cgattttacg tcgacattac gaaccaggtt
    32161 ttcaaattca tcctgagaag cttgcagtga tgatgtttca ctacccactg cacgtgtctc
    32221 aggatgcata ttctttgcgg tgttatttgc actacatgca gaaagcagaa ccgctactgc
    32281 aatcgcggga atcccgcgca agatatatct caaaatcggt tgagatttga ccatgttgtt
    32341 tgttttccct tgaagtcctt aacgacaaat atcgttataa aaaaatgcca agcgacacga
    32401 gactaattac ctgggcatgt tgaaacaatt ccttaaggcc aaatctgtgg actaacgcac
    32461 aaaattatat gctgtgagga taatctaccg ccgaggcaaa acgagttcga gattacccga
    32521 ttggtgcggt cttggcgagc ggttttacat gattttttat aagaaaaagt gatacagaaa
    32581 gttaataagc gggattggtc aattaaagta cgattgagtg aaacgcatgc aatccattca
    32641 tttacttaga gaatgatttt tgcgcgcgat ggatgaaagc acggtgcgca tcatcaggat
    32701 tatcctcact ataaaaataa gcctgatgat gttaattact gtgagttatt tgttttggaa
    32761 atgtttgttt tttcccggta gtgatttgtc aaaaaaggtg attaatcgat cacttaatgg
    32821 cgtcagcaac acccagggca aaccgatcaa tatcacggtc atcgaaccga caatgatgtc
    32881 agtaaaccag tgtgcgccaa tcattactct gggaaatgca aaaaccacaa aaataataag
    32941 ggcgataagg cctgcaactt tgccgaaata acgccacatg aatgccgaaa aaataagcag
    33001 catcatgccg tgatcgccgg gaaagctatc tcgtgaggca tctttcgtgg gaacagagag
    33061 cagttcgctg acgcggttaa tatcggtaaa agtcaatgtt gggctggccc gttttacagg
    33121 aattaatgcc tgacccagct ggtttaatac cactgcagtt aatagcatga ccagacccat
    33181 aatcacgata cgtcgtctgc caggggcgtt ttctttcagc cagaaactca gcatcaacat
    33241 tcccatcgcc agcagagaac aaccgtcgaa agcgcgattg ttggtcaatg caaccagcca
    33301 caaaaaggcc ttactttcga ccagtttctg attaaagaaa taaaaaatat ccgcatcaat
    33361 tggcaaccag aatccatgat taacgggaat ataccaggaa agaaacagcg cgaggccgac
    33421 aatattcaac aacactattt gcggcaaatt tttaatcata ttttctctaa ttatcttact
    33481 gaaaaacgca ggcaacctta cgcggtagtc gctaaacgaa gcttcaacag cgcgctctgc
    33541 aaagcattcc agtcagcttc gctgctggaa atcagctcaa tacggctgtc cggtggcgca
    33601 acgttttgcg tttcaatgtg caggtcatcg ccctgacggt tgattcgtac cagcccttcg
    33661 ggaatacgca gcacgccttt gacgcgttcc accggtgcaa gtcgcgccca ttccagaatg
    33721 ccaatggtgt cgaataccgt atcagcgtcg aatatccagc cgcaggcctg atatccttgc
    33781 ccgctgttca gactgcgacg ccagcgttga tgctctggca ggcttaacgc tgctaaccct
    33841 tttttcacga catgctgatg agaatgcgcg gcgctggcgg gcaactcggc taaattgcga
    33901 cgcggcaaat ccagaagatg accgtcaact ttcccatgct cactgtgaat taattgtcga
    33961 tcgccaccat tttgctgcca ccaacgctgt agcgcttgct cactttcggg cgtcgcacgg
    34021 tcggatttat tggcgacaat gatgtctgcg gcagccagct ggtcacggaa gtttccattg
    34081 ctggcgcttt tttcgtccag cagcaggcgt ggatcgagaa tgcacaaggt ggcgcgcaga
    34141 tctatccacg gttcatagac tggtgcggtt aacagatcga ggatctgttt cggatggccc
    34201 agcccggtcg gctctatcaa caagcggtct ggttttccct gacgcagtaa ggtattcaac
    34261 cctacctgca tgggtaaacc attaacgcag cacatgcagc cgccggggat ctctttcagc
    34321 aatgcgccgc tatcggcgag caaagcacca tcaattccga cttccccaaa ttcattaacc
    34381 agtaccgccc atttttcgtt gggatcttta tgggctaaca gatgaagaat cgacgtggtt
    34441 ttcccgctgc cgagaaaacc ggtgatgagg ttggtcctgg tcacaacggc tccgcaatac
    34501 tctggtaagt taactgagcg ttaatcctgg ctaaaaaagg cagaaaagag aaggggagag
    34561 cggacgaagg tgagcttcat ccgctttaac cgcagaaatc gttaaatttt gagggtgttt
    34621 aatagcgcct gatgtgcgcc gaattgtacg atttgttgcc aggcttgttc gatggcctgc
    34681 acaaaatgcg ggttatctgg cagatcatca ccgaaaactt cacgcaggga aagcagggcg
    34741 gttacgcgtt gttcactgct gctgcccgca acaagttcgc gaattttatc gctaagcgga
    34801 tcgcgaacat caatggcatt tccggcatca tcaacgccgc tgacgtaacg catccagcca
    34861 gcaacgccta atgccagcaa cgaccagtcc gtttcgcgcc ccagatgtat gcgaatacct
    34921 gccagcatac gttgcggtaa tttctggcta ccatccattg cgatttgcca ggtcttatgt
    34981 ttcagcgccg gattagcaaa acgtgcaatt aacttgtccg catattgtgt taaatcgaca
    35041 tctttaattt gcagtgtcgg cgcttgctca tccagcatta atgttctggc ggcatggcga
    35101 aatgcgcgat cctgcatgca atcactgata tgggcgaatc ctgagaggta acccagataa
    35161 gcgagaaaag agtggctgcc attaagcatc cgcagtttca tctcttccca tggcaggaca
    35221 tcattcacca tttgtacacc tgcgacttcc caggcaggac gcccagcgac gaagttatct
    35281 tccaccaccc actggataaa cggttcgcag ctaatcgcgc agggatcatt cacccccaga
    35341 tgctggctga tttccaccag tgattcgtcg gttgcagccg gaacaatgcg gtcgaccatg
    35401 gttcccggaa aactgacgtg ttcttttatc cacccggcga gttctggcga acgtttttct
    35461 gccattccca gcaccgcgtt tttcaccaca tggccattgt cgggaatatt gtcgcaggag
    35521 agcacggtaa acggtgtaag gccgcgctca cggcggcgtt tcagtgcttc gacgagaata
    35581 cccggtgcgg agtgaggttc ttcaggggtt tgtagatcgt gaataatccg cggattactg
    35641 gtgtcgagtg caccggtggc cgggtcaata caatagcctt tttcggtaat cgtcagggaa
    35701 acaattgcca cctgtggctc gcaaaatttc tcaataattg ccgctaagga atccagtttt
    35761 gcattaaggc attcgtgaac ggcaccgaca attatcacct gattgccgtc cgcacctttt
    35821 tccagcacgg tatataaatg gttctgtgcg cggagctggc tcatcagttg atcaccgctg
    35881 aacaagctga tttcacagat cccccagtcg ccgccctggg cattcagcac acgatcggtc
    35941 agtaacgcct gatgagcgcg gtgaaaggcg ccaaagccaa aatgaacgat acgtgattgc
    36001 aactgctggc gatcgtagcg tggagcgtgt acatgatgcg ggagcgtaac ggaggcaatt
    36061 gtcttcatag gagtcacgtc ttagtaacga taaattaaca atgacagtgt aaagttatat
    36121 gacagcaatt gaaattggtc tggattgtta atgcgatgaa gtgtgagggc gatcaatcaa
    36181 cgagtgaaaa ttgagggcgt aaggtgttca ggaagcgcaa aatagaagat tggtataata
    36241 aattaaaaaa gggacagcct gagctatccc ttttctgcat gcggctgaag ttagtcagca
    36301 cgccccatat aacggcgttc ttcgatatgg atacgaattt tttcgcccgg gcttaagtat
    36361 tctggtacct gaatcaccag accagtgctc aatgtcgccg gtttgttacg ggcgctggca
    36421 gatgcccctt tgatgcctgg tgcggtttca acgatttcca gatcaacggt ctgcggcagc
    36481 tcaagcgcca gcagttgacc gtcccaggtc agcacctgca tgtccggcat gccgccctct
    36541 ggcataaaca gcaactcttc ttcaatctga tctttggtga aggtatacgg ggtatagtct
    36601 tctttatcca taaagacata ttcgttgcca tcgacatagg agaagtcaac gtaacggcgg
    36661 gtcagcgtca cggtgtcaac gatatcatca cctttgaaac gctcttctac tttcagcccg
    36721 gtacggacat cagaaaaacg cattttgtac agcgtagcgg caccgcgggc agtgggcgac
    36781 tgaatatcaa tatccttaac cagcagcagt ttgccgttgt aattcagtac catacctttt
    36841 ttaatttcgt tcgctcttgg catcgaaaaa ttcctgtagt cgaaaatgtc aaaaatatcg
    36901 cgacaaagta ctcgcgacgg gcctttcggg caagtggtat tcgcactttt gctggtgcaa
    36961 aaaaggtggt actgtgcgcg ctcatcaatc cggtggttaa ccttaagaga acaacgtatg
    37021 aaatgccgtc cgggttgcgg ggcgtgttgc actgcccctt caatttccag ccctattccc
    37081 ggtatgccag atggcaagcc cgccaatacg ccctgcattc agcttgatga acagcaacgc
    37141 tgtaaaattt tcacctcgcc actgcgaccg aaagtctgtg caggcttaca agccagcgct
    37201 gaaatgtgcg gtaactcgcg tcagcaggcc atgacctggc ttatcgatct ggaaatgctg
    37261 accgcgcctt aaacatcttt aatccgcagt aagcaaaaca gagtggcgat aatcatcacc
    37321 atcgcaaacc agaacacagc gtgataattc cagatctcgg cgacgatgcc cgccactgat
    37381 cctgcgatga tccagcccac gcgcgacgtg ttggtataga gcgtggtggc tgaacccgcc
    37441 tgaccgggca tcagatcctg aaaatagagc atcccgatgc cgcccagaat gccaataaaa
    37501 atagcattta gcagctgcaa gcccaacaga atgacaggtg aatgcgccat cagcattcct
    37561 gcgtaaaaac agacgccacc cacggcagca acgcgcatta agaaacgctt acccagacgt
    37621 ttggcgaaat atccggcaat caacatcgtc gggatttcca gcccggcggc ggtccccatc
    37681 atcacaccgg ccagtttctc gggaagatgc agttcgttga taataaatag cggcatgttg
    37741 atgatgtaca ggctgttcga gccccacatc aatgtacaaa tgacaaacag cagtagcgta
    37801 tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg ccagcggaag ctctttttgc
    37861 atcgacggta aaaacagcca caccatcacg ccgcaaacaa taaacgctac cgctgcgctc
    37921 agatacatta ccgtaaagct gaaacccatc gctaacgcat aagccagcgg tgggccaatg
    37981 acccatgcca gtgaaacctg agcgcgtaaa aaagagctga acatcaccgc ctcacgtccg
    38041 gttttgtcgg catgttcacg ggcaagggca aacatttgcg ggttagcggt cgagccaaag
    38101 ctgctaagaa agacgccaac gaatagcaaa acaaagtagt tgcgattcca ggcaaaaagg
    38161 gtgcaggcca gcacgcctaa caggcagcaa aagacaatca gcgatttgcg atcgccgcgc
    38221 ttatcagagc gcccggcgag aaactgactt accagaatcc caatgacagc gctgccggta
    38281 aagaagaatc ccaccatcgc cggacgggca tgtacttcat cggtaagaaa aatactgagt
    38341 gtcggggttt gcagagcgcc cgcaataccg gtgagaaagg caacgattaa aaacgccgtc
    38401 gaggtcaggt caaacgattt cgcgctggag actgcggggg agttatgcat gctggtcgat
    38461 cactggaaga ggaagacgcg gagtttacgc tgcttgtcag aaaataaaca gacagcgaaa
    38521 tcaaaagcgc cacgaataat caaaatgcca tttcactgtt gctgattggc tgctgacgct
    38581 gctgaaatga aattgctgat aacggatttt cccatcagca attaggaaaa atggcaaaaa
    38641 attgtgcagc acatcaaact tttgctcata actttacggc tttccttgcg tgctgaaaca
    38701 gaagtattat gctttcttga aacgtttcag cgcgatcttg tctttaaccc taagccaggt
    38761 tggcgctttt tttctcatag aggctgaatc gtttcaattc agcaagagag gagaactatg
    38821 ttccagttat ccgtacagga catccatccg ggcgaaaagg ccggagacaa agaagaggcg
    38881 attcgccagg tcgctgcggc gctggtgcag gccggtaatg tagcagaagg ctacgtcaat
    38941 ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg gcaatggtat tgctattcca
    39001 cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg gcgttcaggt atttcagttc
    39061 ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg tggcaatcgg tattgctgcc
    39121 agctcggatg aacatctggg cctgctacgc cagctgaccc acgtactgag cgatgattcc
    39181 gttgctgaac aactgaagtc agcaacaaca gcagaagaac ttcgcgcatt actgatgggc
    39241 gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc tgacgctgga tatcgtcgcc
    39301 agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc tgaaagaggc gggggcagtt
    39361 gacgccactt tcgtcaccaa agccatcaat gaacaaccgc tgaatctcgg acagggtatc
    39421 tggctgagcg atagcgccga aggcaatctg cgtagcgcga ttgcggtaag ccgtgcggca
    39481 aatgcttttg atgtggacgg cgaaacggca gccatgctgg tgagtgtggc gatgaatgac
    39541 gatcagccca tcgcggttct taagcgtctc gctgatttat tgctcgacaa taaagctgac
    39601 cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc tgaccagcga tgatgcgccg
    39661 accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg aacacggcct gcatgctcgt
    39721 ccaggtacca tgctggtcaa taccattaaa caatttaaca gtgatattac cgtgacaaac
    39781 cttgatggta ccggcaaacc ggcaaacgga cgtagtctga tgaaagttgt ggcacttggc
    39841 gttaagaaag gtcatcgcct acgctttacc gcccagggtg cagatgctga acaggcgctg
    39901 aaagcaatcg gcgacgctat cgctgctggt cttggggagg gcgcataatg agcagacgtg
    39961 ttgctactat cacccttaat ccggcttatg accttgttgg tttctgcccg gaaattgaac
    40021 gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc ggcgggtaaa ggcatcaacg
    40081 tggccaaagt attaaaagac ctgggaattg atgtcaccgt tggcggcttc ctgggtaaag
    40141 acaatcagga tggttttcag caactgttca gcgagctggg cattgccaac cgtttccagg
    40201 ttgtacaggg gcgcacccga attaacgtta agctgacgga aaaagacggc gaagtgaccg
    40261 acttcaactt ctcgggtttt gaagtcaccc ccgccgactg ggaacgcttt gtgactgatt
    40321 ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag cggaagctta ccgtcaggcg
    40381 tcagcccgga agcgttcacc gactggatga ctcgcctgcg tagtcagtgt ccttgcatta
    40441 tctttgatag tagccgtgaa gcgttagtag caggtttgaa agcggcaccg tggctggtga
    40501 aacctaaccg ccgcgagttg gaaatctggg caggccgtaa actgcctgaa atgaaagatg
    40561 tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc gcatgttgtt atttcactgg
    40621 gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg gatcgccaaa ccaccgtcag
    40681 tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt tggtggcctg atttatggct
    40741 tgctgatgcg tgaatccagt gaacacacac tgcgtctggc gactgctgtt gcagccctgg
    40801 cggtaagtca aagcaatgtg ggtattaccg atcgtccgca gttggccgca atgatggcgc
    40861 gcgtcgactt acaacctttt aactgacagc aggagaggca taatgaaaac gctgctgatt
    40921 attgacgcta atctcggtca ggcacgcgcc tatatggcga agaccctgct gggcgcggcg
    40981 gcgcgaaaag caaaactgga aatcatcgac aatccgaacg acgcagaaat ggcgattgtt
    41041 ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta aaaatgtctg gctgggtgat
    41101 atttcccggg cagttgcgca ccctgagctg ttcctgagtg aagccaaagg ccatgcgaaa
    41161 ccttacactg cgccggtcac tgcgacagca ccggttgccg ccagcggtcc gaaacgcgta
    41221 gttgcggtga ctgcttgccc gactggcgta gcacacacct ttatggcggc tgaagccatt
    41281 gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg aaacccgtgg ttctgttggc
    41341 gcgggtaatg caatcactcc cgaggaagta gccgcagcgg atctggtgat tgtggcggca
    41401 gatatcgaag tggatctggc gaaatttgct ggtaaaccga tgtatcgtac ctctaccggt
    41461 ctggcgctga agaaaaccgc gcaggaactg gataaagcgg ttgctgaagc aacgccgtat
    41521 gaaccggcgg gcaaaactca aacggcgacc actgaaggta agaaagagag tgcaggcgct
    41581 tatcgtcact tgctaacggg cgtctcttac atgctgccga tggtcgttgc tggtggtctg
    41641 tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta aagagccggg tacgttggca
    41701 gcggcgctga tgcagattgg tggtggttca gcctttgcgc tgatggtacc ggtactggca
    41761 ggttatattg ccttttccat tgccgatcgt ccgggcctca cgccgggtct gattggcggt
    41821 atgctggcgg tcagcaccgg ttctggcttc attggtggta ttattgcggg cttcctggct
    41881 ggttacattg cgaagttaat cagtacgcaa ttgaaactgc cacagagtat ggaggcgctg
    41941 aaaccgatcc tgatcattcc gctaatttcc agtctggtgg tcggtctggc gatgatctac
    42001 ctgatcggta aaccggttgc tggcattctc gaagggttga ctcactggct gcagaccatg
    42061 gggactgcga atgcggttct gctgggggcg atcctcggtg gcatgatgtg tactgacatg
    42121 ggcggtccgg taaataaagc agcgtacgca ttcggtgtgg gtctgctgag tactcaaacc
    42181 tatggcccga tggcggcgat tatggcggca ggtatggtgc caccgctggc aatgggcctg
    42241 gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc aggaaggtgg caaagccgct
    42301 ctggtactgg gactgtgctt tatttcggaa ggtgcaattc cgtttgctgc tcgtgatccg
    42361 atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga ctggcgcaat ctcaatggcg
    42421 attggtgcga aactgatggc accgcacggt ggtctgtttg ttctgctgat ccctggcgct
    42481 attacgccgg tattgggtta cctggtagca attattgccg gtacgctggt ggcgggtttg
    42541 gcctatgcct tcctgaaacg tccggaagtg gacgcagtag cgaaagcagc gtaataaaag
    42601 gtgtgttaca gggcagaaat ttcctgccct gtatcattgg atgagcgcaa agggaatgac
    42661 cggtttgtaa atgtaatttt atgaatttaa atggttttat ctttcagtag aaacctgaaa
    42721 cgttcatcaa acactaaggt tatttcctca agggcagatt attttctgcc cttttttagt
    42781 gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt gtaattcagg catttaatcg
    42841 cattttgtga gcctgctcaa atctgcccat tttgcctctc tggcggtaat ttactttacg
    42901 taaaacatgc atactatgag cacatgttta aatatgaaca catgtttaaa ccgtctttgc
    42961 agtatctata ttcttgtgga ttgacagggt tggtaatttt ttaccagagg acgtgctatg
    43021 cgcgaaaaga attatgtcgt aattataggt tcggcgaata ttgatgtcgc cggatattca
    43081 catgaatcat taaattatgc ggattcaaat ccaggtaaaa taaaatttac gcctggtgga
    43141 gtagggcgca atattgcaca aaacctggcg ttgctgggta acaaagcctg gctactgagc
    43201 gcagtaggca gtgattttta tggtcaatcg ctgctaacgc aaaccaatca atctggcgtt
    43261 tatgtcgata aatgcctgat tgtgccggga gaaaatacgt ctagttattt atcattactc
    43321 gataataccg gtgaaatgct ggttgctata aatgacatga atattagcaa cgctattaca
    43381 gctgaatatc tcgcacagca ccgtgaattt attcagaggg caaaggtcat tgtcgcagac
    43441 tgtaatatca gtgaagaggc actggcatgg attctggata atgccgccaa cgtacccgta
    43501 tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc gcgaccgtct aaatcagatc
    43561 cacactctga agccaaaccg ccttgaagcg gaaacactga gtgggattgc gctgtcaggg
    43621 cgtgaagatg tggcaaaagt tgctgcctgg ttccatcaac atggcctgaa ccgactggta
    43681 ttgagcatgg gcggcgatgg cgtttattac agtgatatca gcggtgaaag tggctggtct
    43741 gccccgatca aaaccaatgt tattaatgtt accggagcgg gcgatgccat gatggcggga
    43801 cttgcttcgt gttgggtaga cggaatgccg tttgccgaat ctgttcgttt cgcacaggga
    43861 tgttcgtcaa tggcgctctc ctgtgaatac accaataacc ccgatttatc gattgccaac
    43921 gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa aatttcccct gaattattac
    43981 aaatttcccc ggaagtgcag gacgctttaa aaaacaaaaa accggttgtg gcgctggaat
    44041 cgaccattat ttctcacggg atgccgttcc cacaaaatgc ccagaccgca attgaagtag
    44101 aagaaactat tcgtaaacag ggcgcagtac ctgccactat cgccattatt ggcggcgtga
    44161 tgaaagtggg tttaagcaaa gaagaaattg aattactggg tcgtgaaggg cataacgtga
    44221 ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc cggaaaaaat ggcgcaacca
    44281 ctgtggcttc aacgatgatt attgcggcgc ttgccggaat taaagtattt gccaccgggg
    44341 gaattggtgg tgtgcatcgc ggggcggaac ataccttcga tatttctgcc gatttgcaag
    44401 aactggcaaa tactaatgtc accgttgttt gtgccggggc gaaatctatt ctcgatttag
    44461 gattaacaac agaatattta gaaaccttcg gtgtgccgtt aattggctat cagacaaaag
    44521 cgctgcctgc gtttttctgc cgtaccagcc catttgaagt cagtattcgc cttgatagtg
    44581 ccacggaaat cgctcgtgca atggcagtga aatggcaaac cggtttgaac ggcggcctgg
    44641 tagtggcgaa tccgatcccg gaacagtttg cgatgccgga ggaatctatc aatgcagcca
    44701 tagatcaagc cgtcgccgaa gccgaagagc agggcgttat tggtaaagaa agtacaccgt
    44761 tcctgctggc gcgcgttgct gagctgaccg gcggtgacag cctgaaatcc aatatccagc
    44821 tggtgttcaa caacgccatt ctggcgagtg aaattgccaa agaataccag cgtctcgcgg
    44881 gttaatttct gacgggcaga aagccgtcgc ccgtactcat caagcatacc aaaacctggc
    44941 gctgacgccg ggtttactgg catgaaggga aaatcattat ggatataatg agaagtgttg
    45001 tggggatggt ggtgttactg gcaatagcat ttctgttgtc agtgaataaa aagagcatca
    45061 gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc tattggtggc atcatgctct
    45121 acttcccacc gggaaagtgg gcagtagaac aggcggcatt aggcgttcat aaagtgatgt
    45181 cttacagtga tgccggtagc gccttcattt ttggttcgct ggttgggccg aaaatggatg
    45241 tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt acttccggcg attattttcg
    45301 ttactgcgct catcagtctg ctgtactaca ttggcgtgat ggggctgctg attcgcatcc
    45361 ttggcagcat tttccagaaa gctcttaaca tcagcaaaat cgaatctttt gtcgcagtca
    45421 caactatttt cctcgggcaa catgagatcc cggcgatcgt taaaccgttt atcgatcgca
    45481 tgaatcgcaa cgagttgttt accgcaattt gtagcgggat ggcgtccatt gctggttcga
    45541 tgatgattgg ttatgccgga atgggcgtac caattgacta cctgttagcg gcatcgctga
    45601 tggcgatccc aggtggtatt ttgtttgcac gtattcttag cccggccacc gagccttcac
    45661 aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc aaaaagcttt atcgaagcgg
    45721 cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg tgtagcgacg gtggtaatgg
    45781 cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg cggaattggt ggctggtttg
    45841 gtttcgccaa tgcctctctg gaaagtattt ttggctatgt gctggcaccg ctggcgtgga
    45901 tcatgggtgt ggactggagt gatgccaatc ttgcgggtag cctgattggg cagaaactgg
    45961 cgattaacga attcgtcgct tacctgagtt tctccccata cctgcaaacg ggcggcacgc
    46021 tggaagtgaa aaccattgcg attatctcct ttgcgctttg tggttttgct aactttggtt
    46081 ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc aaaacgcgcg ccggaaatcg
    46141 cccagcttgg tttacgggcg ctggcagcag caacgctttc caacctgatg agtgcgacca
    46201 ttgccgggtt ctttattggt ttagcttgag tttcattgcc ggatgtatgc gcttattaag
    46261 tgttgcatcc ggcaatcgcg ctattgcaaa taatttttac tgcatcatcc cggagaattt
    46321 attctccggg tccatctcca gcgccagtcc tgacaactgc tttctgtttt taattagata
    46381 ccctttcttg cttttaatta ataaaccgtc gtgaatgaat tgcgcgagaa catataaaag
    46441 atgtcgataa gaaacgccta aatactctgc cgcttgcgta tgcttttcgt gataaagatc
    46501 accttcctgc gagagtaata taaatgctgc caggcgattc actaacggaa atgattgatt
    46561 ctgagttaaa gaaacaatat tacgataatt tttatgactt aaggtgacgc agagttttcg
    46621 taaaaatagc gtgtcgttta ataaccgcgg gcggtaatgt ttcataggga gcgcaaggca
    46681 ccaacactct tcaatagcct gcaccgcacg cggttcatgg tctttgtcga ttaactcaat
    46741 ctcgccaata aaacaggggg cggcaaagaa atcgatcagc gacacgcgac cattagctag
    46801 cgtggcgtaa agcctggcgc ggcctcgcgt caggtaaaac agccaggacg gctgttgccc
    46861 ttcctgcaca atgtagtcac gcgctaaaaa atgaaacagc cgcgtatcgg ctgaaacatc
    46921 cgttaaaaag caatccttaa acgcggattc actcatcaat tgctgcttaa gatcattatt
    46981 gtggatttct ttcacgatgt ttatccgcta tgagatttct catatttagc atacgtacgc
    47041 ggttggtaag attcaatcat cggcccggtc gaattttctt tgctgtagaa aaaataacgc
    47101 aactggaaac agaggaaata aacaatggaa aagagaaaaa ttattctgga ttgtgatccg
    47161 ggtcatgatg atgctattgc aatgatgatg gcagcgaaac atccggcaat agatttatta
    47221 ggcatcacta ttgtagcggg taatcagacg cttgataaaa cattaattaa tggcctgaat
    47281 gtttgccaga aactggagat taatgttccg gtttatgcgg ggatgccgca acccattatg
    47341 cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg gactggatgg cccggtattc
    47401 gagccgctga cccgccaggc agaaagtacc cacgcggtga aatacatcat tgataccctg
    47461 atggcaagcg atggcgatat cactctggtg ccggttggtc cgctttcaaa tatcgcggtg
    47521 gcaatgcgta tgcaacccgc gatcctgccc aaaatccgtg aaattgtgct aatgggcggc
    47581 gcttacggta caggcaactt cacgccatct gccgagttca acatctttgc tgacccggaa
    47641 gcagcccgcg tggtgttcac ctctggcgtt ccattagtga tgatgggcct cgatcttacc
    47701 aaccagaccg tttgcacccc ggacgtgatt gctcggatgg aaagggcagg cggccctgcc
    47761 ggagagctgt tcagcgacat catgaacttc actctcaaaa cgcagttcga aaactacggc
    47821 ctggctggcg gcccggtgca cgacgccacc tgcatcggtt atctgattaa ccctgatggc
    47881 attaaaaccc aggagatgta cgtcgaagtg gacgtcaaca gtggcccttg ctatgggcgc
    47941 accgtctgcg acgagctggg cgttcttggc aagcccgcca ataccaaagt cggcatcact
    48001 attgataccg actggttctg gggcttagtc gaagagtgcg tgcgcggcta catcaaaacc
    48061 cattaagcgt tgataacaag ccgggccgat agcgcaagat gcaggcccgg ctatttctga
    48121 tgggcagaaa gccgtcgccc aatatgacat gaagagataa tcactatgga tgtcatgaga
    48181 agtgttctgg gaatggtggt attgctggca attgcatttt tgctgtcagt aaacaagaag
    48241 aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac aggttgtgat tggcggcatt
    48301 atgctttggt taccgccagg gcgttgggtc gctgaaaaag tcgcttttgg cgtgcataaa
    48361 gtgatggcgt acagcgacgc gggtagcgca tttatcttcg gttcgctggt cggtccgaaa
    48421 atggacacgc tgtttgatgg cgcaggattt atctttggtt tcagagtatt accggcaatt
    48481 atcttcgtta cagcactggt gagtattctc tactacatcg gtgtgatggg gattttaatt
    48541 cgcattctcg gcggtatctt ccagaaagcg ttaaatatca gcaagatcga gtcattcgtc
    48601 gcggtcacta ccattttcct cgggcaaaac gaaattccgg caatcgtcaa accctttatc
    48661 gatcggctga atcgcaatga attatttaca gcgatttgta gtggcatggc ctcgattgct
    48721 ggttcgacaa tgattggtta cgccgcactg ggcgtgcctg tggaatatct gctggcggca
    48781 tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc tgttaagtcc ggcaacggaa
    48841 tcttctcagg tttcctttaa taacatctct ttcaccgaaa caccgccaaa aagcattatt
    48901 gaagccgctg cgacaggggc aatgaccggg ctgaaaatcg ccgccggtgt agcgacagtt
    48961 gttatggcat ttgtcgccat cattgcgtta attaacggta ttatcggcgg cgttggcggc
    49021 tggtttggtt ttgaacatgc ctcgctggag tccattttag gttacctgct ggccccactt
    49081 gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg ccgggagttt gattggacag
    49141 aaactggcaa taaatgaatt tgtcgcttat ctcaatttct caccctatct gcaaacggct
    49201 ggcactctgg atgctaaaac cgtggcgatt atttccttcg cgttgtgcgg tttcgctaac
    49261 tttggttcta tcggggtggt ggtgggggcg ttttctgcgg ttgcgccaca ccgtgcgccg
    49321 gaaatcgccc agcttggttt acgcgcgctg gcggcggcga cactttctaa tctgatgagt
    49381 gccaccattg ccgggttctt tattggttta gcgtagtgcg caatttatga gccggataag
    49441 atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat tggcactaat gaaagcgcgt
    49501 tatcggcaga cagggtaggg ttgtttaagc tgccgctggc gcgcgaaatt gccgcgcagg
    49561 ctaccgcaaa acgggcgctg tcgcggaaac tgtatccttc aagaaagcta tataccaagc
    49621 ccgccataaa accatcgtca gcaccaaaac tgtcaactgt cgtatgcgct ggcgcggtca
    49681 gcaaaaattg ttctccatcc ttttcgctgc aataaactga ctcatcgggc aaataaacaa
    49741 acagttgctg aacaccttgc tgatgcaatg cattcactgc ggcattacgg tcagcatcgc
    49801 tggtgatcgc ctgtccccat aaaatttcca gctccggtaa agtgggtttc agggtgtgaa
    49861 tatgcgccag ccagtgtttg attttgcccg ctttgaattc tgaaacggta tcgacaaaca
    49921 ccgggatttc atcagcgagg gtaaagaccc attccagcgc ctcggctgtc aggttgcaat
    49981 cagccagtac cacgcccgca tgacgaagta aatcgcgcga cccgttcagt agttgcggtg
    50041 tcaactgttc cagcagatgg gtatcgttaa tcgccagcac ggtttgatcg tctcgattgg
    50101 caattgccag atacgtcgat gtgctttgac catgcaaacg aacgcagccg gagacattca
    50161 cgccggcacg gcgcgtttct tccaggagca tttcgccata aaagtcatcg ccaatcactg
    50221 aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg ggcgatgttg cgtcccacgc
    50281 cgcccgccga gcaatgaatt gtaccgggat gagaagccgc ttgcgggtaa cggatatccg
    50341 ccatcccgcg aatatccata ttgattgtcc ccaccactat gcagtattcc tgctcggtga
    50401 gaatgtaacc tttgccttta atccgccctt tgcgcattaa atccataata tgcgccgcaa
    50461 cgcgcgaacg actgatttgc agcatgtccg caatttcgtt ctgctgaatc agcgggttac
    50521 gccgtaaaat tgcaaggatc tccttttccc ggttattcat cttcaggcta ccgctttttc
    50581 agtttgttgc gctttcagcc aggcgatctc ttctgcccag atatccgggt tgatggtttc
    50641 aaggatcagc ggaatgccgt cgaaacggtc gtcttgcatg atccagcgga acgcatcatg
    50701 accgatattg ccttcaccga ggctatgatg gcggtcaacg cggctgccaa aggtactttt
    50761 cgcatcgtta aggtgcatcc cgcgcagata cttaaagcca acaatacggg caaaatccgc
    50821 gaacgttttc tcgcattcgg ctggagtacg caaatcatac ccggcagcga aagcatggca
    50881 ggtatcaatg cagacgccga cacgggattt atcttccacg ccgtcgataa tcgccgcgag
    50941 atgttcgaat ttaaacccta agttactgcc ctgaccggcg gtgttttcaa tcaccgctgt
    51001 tacaccttga gttttatcca gcgcaatgtt gatggattcg gcaatacgcg caaggcaatc
    51061 ctcttctgaa atctgcatca gatggctgcc agggtggaag ttgagcaaag aaagccccag
    51121 ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc gatttttcca gagcttcagt
    51181 gaccggatgt ccgaggttaa tcagatagct gtcgtgggga agaatttgcg ccgatgtgta
    51241 gtgatatttt tcacaggccg ctttgaattc atcgatggtt tgcgtcgtga gcggtgcggc
    51301 acgccactga cgttggtttt tggtgaacaa ggcaaacgcg gttgcgtcga tttcggcggc
    51361 gcgaattgcg gcatttgcca gaccgccagc agcactaacg tgcgctccaa tgtatttcat
    51421 gcgaggactc ctgttaaacc cgctggagga aaacggtaat gatagcgggt taacaggagc
    51481 aagatgtagt ggtttatgcg atgacgcttt gaatcacata gttaatcgca ccaccaccaa
    51541 caatcagcca ggcaaacagt accagtgcca tcagcagagg tttcgccccg gcttttttca
    51601 gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc cattgccagc aggaaggtat
    51661 ccagcgttac cagcatgttc accacgctct gcggtaacag gtggaacgag ttaaagatgg
    51721 caacgacgat gaacaagatg gcaaaccacg gaatagtgat tttgcttttc tcgccgctgt
    51781 tcgccccaga cagctgttta acacgcgccg ccagcaggat gaggaacgga gccagcatca
    51841 tcacgcgcag cattttggaa ataactgctg cgttttccgc atccgggctg atggcatgac
    51901 ctgccgccac cacctgcgcc acttcgtgca cagtagaacc aatgtagata ccgaaagttt
    51961 ccggactaaa ccattgagac atcagcggat atatcgccgg gtagaggaaa atcgcgacgg
    52021 tcccgaagat aacaacggtt gcaacagcca cggttacttt actggcttcc gctttcacta
    52081 ccggctcagt cgccagtacc gcggcagcac cacagatact gctaccggca ccgatcaacc
    52141 agctggtgtg cttatccaga ccaaacactt tctgccccag gaagcaagcc agcaggaagg
    52201 tactggacag cgtcaacacg tcaatgatga tcccactgat accgacatcg gcaatttgcg
    52261 agaacgtcag acggaagcca taaagaatga tacccagacg taataaatat tgcttggcaa
    52321 acagcacacc accgtcacag cttttccaga tgtgcggata gatggtgttg cctaaaacca
    52381 tccccaacaa gattgcgagg gtgagggcac taaacccggc acccgcaacc gcgggaatgg
    52441 aaccacccca cagggcgacc ccggtgataa ctgcactcag ggctaacccc ggaataaaat
    52501 gccacagtgt acgatgttgt ttctgtaagg tgatattcgt cataaccctc tcctttaacc
    52561 gggcataagg ttacgactaa ctggtttaaa aataaaat
//
LOCUS       DAPGWD010000014        51430 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPGWD010000014 DAPGWD010000000
VERSION     DAPGWD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.014"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            139..444
                     /locus_tag="RHX88_000787"
     CDS             139..444
                     /locus_tag="RHX88_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HDV2229515.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            551..1195
                     /gene="gfcB"
                     /locus_tag="RHX88_000788"
     CDS             551..1195
                     /gene="gfcB"
                     /locus_tag="RHX88_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HDV2229516.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1192..1938
                     /locus_tag="RHX88_000789"
     CDS             1192..1938
                     /locus_tag="RHX88_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV2229517.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            1938..4034
                     /locus_tag="RHX88_000790"
     CDS             1938..4034
                     /locus_tag="RHX88_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV2229518.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4080..5219
                     /locus_tag="RHX88_000791"
     CDS             4080..5219
                     /locus_tag="RHX88_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDV2229519.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5207..5653
                     /gene="etp"
                     /locus_tag="RHX88_000792"
                     /gene_synonym="yccY"
     CDS             5207..5653
                     /gene="etp"
                     /locus_tag="RHX88_000792"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDV2229520.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5673..7853
                     /gene="etk"
                     /locus_tag="RHX88_000793"
     CDS             5673..7853
                     /gene="etk"
                     /locus_tag="RHX88_000793"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDV2229521.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNNVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(7968..9266)
                     /gene="appA"
                     /locus_tag="RHX88_000794"
     CDS             complement(7968..9266)
                     /gene="appA"
                     /locus_tag="RHX88_000794"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDV2229522.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9346..9438)
                     /gene="cbdX"
                     /locus_tag="RHX88_000795"
     CDS             complement(9346..9438)
                     /gene="cbdX"
                     /locus_tag="RHX88_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDV2229523.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9451..10587)
                     /gene="appB"
                     /locus_tag="RHX88_000796"
     CDS             complement(9451..10587)
                     /gene="appB"
                     /locus_tag="RHX88_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV2229524.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10599..12143)
                     /gene="appC"
                     /locus_tag="RHX88_000797"
     CDS             complement(10599..12143)
                     /gene="appC"
                     /locus_tag="RHX88_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDV2229525.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12277..13134)
                     /locus_tag="RHX88_000798"
     CDS             complement(12277..13134)
                     /locus_tag="RHX88_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDV2229526.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLTVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13131..13529)
                     /gene="hyaE"
                     /locus_tag="RHX88_000799"
     CDS             complement(13131..13529)
                     /gene="hyaE"
                     /locus_tag="RHX88_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDV2229527.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVESQQERAS"
     gene            complement(13526..14113)
                     /locus_tag="RHX88_000800"
     CDS             complement(13526..14113)
                     /locus_tag="RHX88_000800"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDV2229528.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            complement(14110..14817)
                     /gene="hyaC"
                     /locus_tag="RHX88_000801"
     CDS             complement(14110..14817)
                     /gene="hyaC"
                     /locus_tag="RHX88_000801"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDV2229529.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14836..16629)
                     /gene="hyaB"
                     /locus_tag="RHX88_000802"
     CDS             complement(14836..16629)
                     /gene="hyaB"
                     /locus_tag="RHX88_000802"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDV2229530.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16626..17744)
                     /gene="hyaA"
                     /locus_tag="RHX88_000803"
     CDS             complement(16626..17744)
                     /gene="hyaA"
                     /locus_tag="RHX88_000803"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDV2229531.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18171..18258
                     /locus_tag="RHX88_000804"
     tRNA            18171..18258
                     /locus_tag="RHX88_000804"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18205..18207,aa:Ser,seq:tga)
     gene            18465..19124
                     /gene="yccA"
                     /locus_tag="RHX88_000805"
     CDS             18465..19124
                     /gene="yccA"
                     /locus_tag="RHX88_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDV2229532.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            19215..19544
                     /gene="tusE"
                     /locus_tag="RHX88_000806"
     CDS             19215..19544
                     /gene="tusE"
                     /locus_tag="RHX88_000806"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDV2229533.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAIANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(19541..19819)
                     /gene="yccX"
                     /locus_tag="RHX88_000807"
     CDS             complement(19541..19819)
                     /gene="yccX"
                     /locus_tag="RHX88_000807"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDV2229534.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            19914..21104
                     /gene="rlmI"
                     /locus_tag="RHX88_000808"
     CDS             19914..21104
                     /gene="rlmI"
                     /locus_tag="RHX88_000808"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDV2229535.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            21162..21479
                     /gene="hspQ"
                     /locus_tag="RHX88_000809"
     CDS             21162..21479
                     /gene="hspQ"
                     /locus_tag="RHX88_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDV2229536.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(21524..21937)
                     /locus_tag="RHX88_000810"
     CDS             complement(21524..21937)
                     /locus_tag="RHX88_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDV2229537.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            22110..22772
                     /locus_tag="RHX88_000811"
     CDS             22110..22772
                     /locus_tag="RHX88_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDV2229538.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22868..23326
                     /gene="mgsA"
                     /locus_tag="RHX88_000812"
     CDS             22868..23326
                     /gene="mgsA"
                     /locus_tag="RHX88_000812"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDV2229539.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23358..25412)
                     /gene="helD"
                     /locus_tag="RHX88_000813"
     CDS             complement(23358..25412)
                     /gene="helD"
                     /locus_tag="RHX88_000813"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDV2229540.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            25535..25981
                     /locus_tag="RHX88_000814"
     CDS             25535..25981
                     /locus_tag="RHX88_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDV2229541.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25991..28153
                     /gene="yccS"
                     /locus_tag="RHX88_000815"
     CDS             25991..28153
                     /gene="yccS"
                     /locus_tag="RHX88_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDV2229542.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(28116..28745)
                     /gene="sxy"
                     /locus_tag="RHX88_000816"
     CDS             complement(28116..28745)
                     /gene="sxy"
                     /locus_tag="RHX88_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDV2229543.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            28964..29473
                     /gene="sulA"
                     /locus_tag="RHX88_000817"
     CDS             28964..29473
                     /gene="sulA"
                     /locus_tag="RHX88_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDV2229544.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            29830..30882
                     /gene="ompA"
                     /locus_tag="RHX88_000818"
     CDS             29830..30882
                     /gene="ompA"
                     /locus_tag="RHX88_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDV2229545.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(30957..31409)
                     /gene="matP"
                     /locus_tag="RHX88_000819"
     CDS             complement(30957..31409)
                     /gene="matP"
                     /locus_tag="RHX88_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDV2229546.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKHDLQALLGKE"
     gene            31595..33355
                     /locus_tag="RHX88_000820"
     CDS             31595..33355
                     /locus_tag="RHX88_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDV2229547.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            33424..33942
                     /gene="fabA"
                     /locus_tag="RHX88_000821"
     CDS             33424..33942
                     /gene="fabA"
                     /locus_tag="RHX88_000821"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDV2229548.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(34012..34179)
                     /gene="rmf"
                     /locus_tag="RHX88_000822"
     CDS             complement(34012..34179)
                     /gene="rmf"
                     /locus_tag="RHX88_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDV2229549.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(34435..34998)
                     /gene="pqiC"
                     /locus_tag="RHX88_000823"
     CDS             complement(34435..34998)
                     /gene="pqiC"
                     /locus_tag="RHX88_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDV2229550.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(34995..36635)
                     /gene="pqiB"
                     /locus_tag="RHX88_000824"
     CDS             complement(34995..36635)
                     /gene="pqiB"
                     /locus_tag="RHX88_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDV2229551.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(36640..37893)
                     /gene="pqiA"
                     /locus_tag="RHX88_000825"
     CDS             complement(36640..37893)
                     /gene="pqiA"
                     /locus_tag="RHX88_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDV2229552.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(38023..39930)
                     /locus_tag="RHX88_000826"
     CDS             complement(38023..39930)
                     /locus_tag="RHX88_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2229553.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(39941..42049)
                     /gene="rlmKL"
                     /locus_tag="RHX88_000827"
     CDS             complement(39941..42049)
                     /gene="rlmKL"
                     /locus_tag="RHX88_000827"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDV2229554.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSATLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            42293..43402
                     /gene="ycbX"
                     /locus_tag="RHX88_000828"
     CDS             42293..43402
                     /gene="ycbX"
                     /locus_tag="RHX88_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDV2229555.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(43399..43941)
                     /gene="zapC"
                     /locus_tag="RHX88_000829"
     CDS             complement(43399..43941)
                     /gene="zapC"
                     /locus_tag="RHX88_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDV2229556.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(44115..45125)
                     /gene="pyrD"
                     /locus_tag="RHX88_000830"
     CDS             complement(44115..45125)
                     /gene="pyrD"
                     /locus_tag="RHX88_000830"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDV2229557.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(45236..45973)
                     /locus_tag="RHX88_000831"
     CDS             complement(45236..45973)
                     /locus_tag="RHX88_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV2229558.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(45939..46454)
                     /locus_tag="RHX88_000832"
     CDS             complement(45939..46454)
                     /locus_tag="RHX88_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2229559.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(46462..47004)
                     /locus_tag="RHX88_000833"
     CDS             complement(46462..47004)
                     /locus_tag="RHX88_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2229560.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(47016..48086)
                     /locus_tag="RHX88_000834"
     CDS             complement(47016..48086)
                     /locus_tag="RHX88_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2229561.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(48077..50677)
                     /locus_tag="RHX88_000835"
     CDS             complement(48077..50677)
                     /locus_tag="RHX88_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV2229562.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSTAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(50702..51403)
                     /locus_tag="RHX88_000836"
     CDS             complement(50702..51403)
                     /locus_tag="RHX88_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2229563.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT        12475 a        13543 c        12691 g        12721 t
ORIGIN      
        1 tctgcgcgcc tttcctaccc gcagtatata gtgcaagcgg tatgaggaaa gggagatttc
       61 acaaggatgt tatactcaaa gtagacaatc ctgatctcaa gcgtacgtat tgtccatgcg
      121 atgaataaag gaaaagttat gaaacacaag ctttctgcaa tccttatggc cttcatgttg
      181 acgacgccag ctgcgtttgc tgcccctgaa gcaacaaacg ggaccgaggc aacgacgggt
      241 actactggca ccacaacgac cactaccggc gcaaccacga ctgctactac cactggtggc
      301 gtggctgctg gcgctgtcgg cacggcgaca gttgtcggtg tggcaacggc tgttggtgtg
      361 gcgacgttgg cagttgttgc agcaaatgac agtggcgatg gcggttctca taacacctca
      421 accaccacgt ccactacacg ctaatactgc tttaacgcgt cgaaattaaa gttaaatgat
      481 acccggttgc gatattcgta ccgggcttta gagatttgca tttttttaat taacctttca
      541 aggattaaag gtgcgccctc ttattttatc gattttcgca ctatttcttg cgggatgtac
      601 gcacagccag caaagtatgg tcgatacatt tcgcgccagc cttttcgata atcaggatat
      661 caccgtagcg gatcagcaga tccaggcgtt gccttattcc acgatgtatt tacgccttaa
      721 tgaagggcaa cgaatctttg tggtactggg atatatagaa caagaacaaa gcaaatggtt
      781 atcccaggat aacgccatgc tggttaccca caatggacgt cttttaaaaa ccgtcaaact
      841 taataataat ctgctggaag tgacaaattc cgggcaggac cctctgcgta acgcgctggc
      901 aataaaagat ggcagccgct ggacgcgcga tattctctgg agtgaagaca accattttcg
      961 ctctgcgacc ctgagttcta ctttttcctt tgctggatta gagacgctga atattgcggg
     1021 tcgcaatgtg ctgtgtaatg tctggcagga agaggtgact tccacgcggc cagaaaaaca
     1081 gtggcaaaac acattctggg tcgattcggc tactggccag gttcgtcaaa gtcggcaaat
     1141 gttaggcgca ggggttattc ccgtagaaat gacgtttctt aaacccgcac catgaataaa
     1201 ttacagtcgt atttcattgc cagcgtactt tacgtaatga caccccatgc ctttgcgcaa
     1261 ggaacggtga ctatttatct gcctggcgaa caacagacac tttccgttgg ccccgtggaa
     1321 aatgttgtcc agttggtgac acaaccgcaa ctgcgcgatc gcctctggtg gcctggtgcc
     1381 ttgctgaccg actctgcggc gaaagctaaa gcgcttaaag actaccaaca tgtcatggca
     1441 cagcttgctt cctgggaagc agaagctgac gacgatgtgg cggcaacgat taaatccgtg
     1501 cgccagcagt tactcaatct caatatcact ggtcgcttac cggtcaaact ggaccctgat
     1561 tttgttcgag tggatgagaa cagcaatccc ccgttggtcg gggactatac gctctatacc
     1621 gtacagcgtc cggtgactat tactctgctg ggtgcggttt ctggggctgg gcaattgccc
     1681 tggcaggcag gccgtagcgt taccgattat ctgcaagatc acccccgcct tgcaggcgcg
     1741 gacaagaata acgttatggt gattacgcct gagggtgaga ctgtcgtcgc ccctgttgcc
     1801 ctgtggaata aaagacatgt ggaaccaccg cctggcagcc agctttggtt gggcttttct
     1861 gcgcatgtac tgcccgaaaa atatgctgat ttaaatgacc agattgtgtc tgttttaacg
     1921 caacgtgttc ctgactgatg aagaagaatt cttatctttt aagctgcctg gccattgccg
     1981 tctccagtgc ctgtcatgct gaagtattaa cctacccgga tccgctgggt tcgtcgcaat
     2041 cagactttgg cggcacagga ttgttgcaga tgccaaatgc gcgcatcgca ccggaaggtg
     2101 aattcagcgt caactaccgg gataacgatc aataccggtt ctactccacc tccgtggcgc
     2161 tgttcccatg gctggaaggc accattcgtt atacggatgt gcgcacacgc aaatatagcc
     2221 agtgggaaga tttcagcggc gatcagtcat acaaagacaa atcattcgat tttaaacttc
     2281 gcctgtggga agaaggttac tggctaccgc aagtggcgtt tggtaaacgt gatattgctg
     2341 gtacgggtct gtttgacggt gagtatctgg tggccagcaa gcaagcgggg ccatttgatt
     2401 tcaccctcgg gatggcatgg ggctacgccg gtaatgcggg caatattacc aacccgtttt
     2461 gccgggtgag cgataaatat tgtcatcgcg cagagtctca cgatgcgggc gatatcagct
     2521 ttagcgatat ctttcgtggc ccggcttcca tctttggcgg cattgagtat caaacgccgt
     2581 ggaatcccct gcgtctgaaa ctcgaatacg atggcaacaa ttaccagaat gatttcgctg
     2641 gcaaactgcc tcaggcaagc catttcaacg tcggcgcagt ttatcgcgct gccagctggg
     2701 cagatctcaa cctgagttat gaacgcggta acacgttgat gtttggcttc acgttacgga
     2761 ccaatttcaa cgatctgcgc cctgccctgc gcgatacgcc aaaaccggca tatcaacctg
     2821 cgcctgaatc tgaaggattg cagtacacca cagtagcgaa ccaacttacc gcgctgaagt
     2881 acaacgcagg ttttgaagca ccggaaattc agctgcgcga taagacgctg tatatgtctg
     2941 gtcaacaata caaataccgt gattctcgcg aagcggtcga tcgtgccaac cggattctgg
     3001 tgaataacct gccgcaaggc gttgagaaga ttagcgtgac gcaaaagcgc gagcatatgg
     3061 cgatggtgac taccgaaacc gacgtagcca gcctgcgcaa acagctggct ggtacagcgc
     3121 ctggtcaatc agagcaactt caacaacaac gtgttgaagc agaagatctt tctgcctttg
     3181 gtcggggcta ccgtattcgt gaagatcgct ttagctactc tttcaaccca acactttcac
     3241 agtcgctggg cggcccggaa gatttctata tgttccagct ggggctgatg tccagtgccc
     3301 gctactggtt taccgaccac ctgctgcttg atggcggtat tttcaccaat atttacaaca
     3361 actacgacaa gtttaagtct tcgctgttgc ccgcggactc taccctgccc cgcgtgcgca
     3421 cgcatatccg tgactacgtt cgcaatgacg tttatctcaa caacttgcag gcgaactatt
     3481 ttgctgactt aggcaatggt ttctatggcc aggtgtatgg cggttatctg gaaacgatgt
     3541 atgccggtgt cggttccgag ctgctttatc gcccgctaga tgccagctgg gcgctgggtg
     3601 tggacgttaa ctacgtgaag cagcgtgact gggacaacat gatgcgtttc accgattatt
     3661 ccacgccaac aggtttcgtg acggcttact ggaacccgcc gacgctcaat ggcgtactga
     3721 tgaaacttag cgttgggcaa tatctggcaa aagataaagg cgcaacgatc gacgtcgcca
     3781 aacgctttga cagcggcgtg gcggtagggg tatgggcggc aatcagtaac gtatctaaag
     3841 atgactacgg cgaaggcggc tttagtaaag gtttttatat ctcgattcca ttcgacttga
     3901 tgaccattgg acctaaccgc aaccgcgcgg tggtttcgtg gacaccattg acgcgtgatg
     3961 gtggacaaat gctgtcacgc aaataccagc tctatccaat gacggcagag cgagaagtac
     4021 cggttggaca ataacctgac gtaattcata acattacatt gactgataaa tataaaccaa
     4081 tgaaaaagaa catttttaag ttttcggtac tgacattggc tgttctgtct ttgacagcat
     4141 gtacccttgt tcctggtcag aatttatcga ccagtaacaa ggatgtcatt gaactacctg
     4201 acaatcagta tgacctggac aaaatggtca atatctatcc cgtcacgcca ggattgattg
     4261 atcagttgcg cgccaaaccg attatgtcgc aggctaaccc ggagctggag cagcaaatcg
     4321 caaactatga atatcgtatc ggcattgggg atgtgttgat ggttacggtc tgggatcacc
     4381 cggaactaac cacccccgca gggcaatacc gtagcgccag tgataccggc aactgggtta
     4441 acgccgatgg tgcgattttc tacccctata ttggtcgcct gaaagtggcc gggaaaacac
     4501 ttacccaggt acgcaacgag attacagcgc gtctggattc agtcattgaa agcccgcaag
     4561 tcgatgtcag cgtcgcggcg ttccgctcgc aaaaagcgta cgtcaccggc gaagtcagca
     4621 aatcgggtca gcagccgatt accaatattc ctctgaccat catggatgcg attaacgccg
     4681 cgggcggtct gaccgcagat gcagactggc gcaacgtggt gctcacccag aatggcgtaa
     4741 agaccaaagt gaacctctat gcgctgatgc agcgtggcga cttgcgccag aacaaactgc
     4801 tgcacccggg cgatatttta tttattccgc gcaacgatga tctgaaagtc ttcgtgatgg
     4861 gtgaagtggg taaacagagc acgctgaaaa tggaccgcag cggtatgacc ctggcggaag
     4921 cgctgggtaa cgctgagggt atgaatcagg atgtcgctga tgcgacgggg atttttgtta
     4981 ttcgtgcaac gcaaaacaag cagaatggca aaatcgccaa tatttatcag ctgaatgcga
     5041 aggacgcatc ggcaatgata ctcggaacgg aattccagct tgaaccttac gacattgttt
     5101 atgtcactac tgcgccgctg gctcgttgga accgtgtgat ttctctgctg gtaccgacga
     5161 tttccggggt gcatgacctg acagagactt cacggtggat tcaaacatgg cccaactaaa
     5221 atttaactca atcctggtgg tttgtaccgg caacatttgc cgttcgccca ttggcgagcg
     5281 cttgctgcgt aaacgtctgc caggcgtaaa agtcaaatcg gcaggtgttc atggtctggt
     5341 aaaacaccct gcagatgcga cagcagcaga tgttgccgct aatcatggcg tatctcttga
     5401 aggacatgcc ggacgtaagc tcactgctga gatggcacga aattacgatc tgattctggc
     5461 aatggagtca gagcatattg ctcaggttac cgcaatcgct cccgaggtcc gcgggaagac
     5521 aatgctgttt gggcaatggc tggaacagaa agagatcccg gatccctatc gtaaaagtca
     5581 ggacgcattt gaacatgtct acggtatgtt ggagcgcgcc agtcaggaat gggcgaagcg
     5641 cctcagccgg taatttgagt ttataaaata cgatgacaac taaaaatatg aatacgccac
     5701 caggcagcac tcaggaaaat gagatcgatc tgcttcgtct ggtcggcgag ttatgggatc
     5761 accgtaagtt tattatcagc gtgaccgcgt tattcacgct gatcgctgtc gcttactcgc
     5821 tgttaagtac accaatttat caggcagata ctctggtcca ggttgagcaa aaacagggca
     5881 acgccattct cagcggcctg agcgatatga tccctaactc atcgcccgag tctgcaccgg
     5941 agatccaact gctgcaatcg cgcatgattc tcggtaaaac cattgctgaa ctgaatctgc
     6001 gcgacatagt tgagcagaag tattttccga ttgtgggtcg cggctgggcg agattaacca
     6061 aagaaaaacc aggtgagctg gcgatcagct ggatgcatat tccacaactg aatggtcagg
     6121 atcagcaact gacactcacg gttggggaaa acggccacta tacactggaa ggtgaagagt
     6181 tcaccgtcaa tggtatggtc ggccagcgtc tggaaaaaga tggcgttgcg ctgactatcg
     6241 cggacattaa ggccaaacca ggaacacagt ttgtcctgag ccagcgtacc gaactggaag
     6301 cgattaacgc attgcaggaa acctttaccg ttagcgaacg cagtaaagaa agcgggatgc
     6361 tggaacttac catgactggt gatgatcccc agttgattac tcgtattctg aacagcatcg
     6421 ctaacaacta tttgcaacag aatatcgctc gccaggcggc gcaggattca caaagccttg
     6481 aattcttaca gcgccagtta cctgaagtgc gcagcgagct ggaccaggcg gaagaaaaac
     6541 tcaacgttta tcgccagcag cgcgattcgg ttgaccttaa cctggaagcc aaagccgttc
     6601 ttgagcagat tgtgaacgtt gataatcaac tcaatgagct gaccttccgc gaggcagaga
     6661 tctcccagct gtataagaaa gatcacccaa cttatcgtgc gctgctggaa aaacgccaga
     6721 cgctggagca agaacgcaaa cgcctgaata agcgggtatc ggcaatgcct tccacccaac
     6781 aggaagtgtt gcgtttaagt cgtgacgtag aagcgggccg tgcggtatat ctgcaattac
     6841 ttaaccgcca gcaggagttg agtatttcga aatccagtgc cattggtaac gtgcggatta
     6901 tcgacccggc agtcactcag ccgcagccag tgaaaccgaa aaaagcgttg aatgtggtgc
     6961 ttggttttat tcttggcctg tttatttctg tgggtgccgt gctggcgcgt gcgatgttgc
     7021 gtcgtggtgt agaagccccg gaacaactgg aagagcacgg catcagcgtt tatgccacta
     7081 tcccaatgtc cgagtggctg gataaacgca cccgtctgcg taagaaaaat ttattttcta
     7141 atcagcagcg ccatcgtact aaaaatatcc ccttcctggc ggtggataac ccggcggatt
     7201 ctgctgtgga agccgtacgt gcgctacgaa ccagtctgca tttcgctatg atggagacgg
     7261 agaataacat tctgatgatc accggtgcga cgccagacag tggtaaaacg tttgtcagtt
     7321 caactctggc agcggtgatc gcccagtccg atcaaaaagt gttatttatt gatgccgact
     7381 tacgccgtgg ttattcgcat aacctgttta ccgtgagtaa tgaacatggc ttgtcggaat
     7441 atctggcagg taaagatgag ctcaacaatg tgatccagca ttttggcaaa ggaggctttg
     7501 atgtgattac tcgcggtcag gtgccaccta acccgtctga actgctgatg cgcgatcgga
     7561 tgcgtcaatt actggaatgg gcgaacgacc attacgatct ggtgattgtc gatacgccgc
     7621 cgatgctggc ggtgagtgat gccgcggtcg tggggcgttc tgttggcacc agcctgctgg
     7681 ttgcgcgttt tggcttgaac accgccaaag aggtgagttt gtcaatgcag cgtctggaac
     7741 aggcaggcgt caatattaaa ggcgctatcc tcaatggtgt gattaaacgc gccagcaccg
     7801 cttacagtta cggctataac tattacggtt atagttactc cgagaaagag taaaactgaa
     7861 agtggccgga tgcggcgtga acgccttttc cggtctacgc attagacagt tcttcgtttg
     7921 tcatcagcct cagagcattc aggtaactga atgctctttt ttatgcatta caaactgcac
     7981 gccggtatgc gtgcttcatt cacgatttgc gtaaaacctg ccaacgaaca catgccctgc
     8041 gcatttcgct cttcacatcc tgccagggtc agtttcacct ctccgggcgg cgtatttaat
     8101 gacagcggcg ttttatcacg catctgctgt aaagtctgga agaccagcga aacctgaatc
     8161 cactggctgt tatcgcttag ccgacgccag cgttcaaaca ccagttcacc acctggcggc
     8221 gtgttatccg gctgaccggg aagcgtccag ttgagctcca gtgcgccgcc gagatttgcc
     8281 agattagtat cgtgtccggc gataaacagc actgaagtgg gtaatgtcac accatacgcc
     8341 tgtttttgcg gtggatgggg cgtcaacgct gccatgatca aatccaataa cggggtggcg
     8401 cggctgcggg caacctctgg cgtgcgttgt agcaaataaa attgcgcgtt atgcaaactt
     8461 agcaaggtgt tccactggtg tgaatcggtg atccttcccc accccggctc cggcattccc
     8521 tgtgcttgtt gcaggagaaa tatctccgtc agcattgatg cgaggcttac cgcaccggtt
     8581 aatgagacat tgtcggcgct caccttgagt tccgatggta atgcctgcgt taatgaacag
     8641 ctttcgtcct gtttctcacg tttaaggcac aagtttgatt gcggaaaatt aagcacccgt
     8701 tccagttcgc gaaacgccgt ttgccgatgc ccggtaaagt cagcaattga ccctcctgcc
     8761 ctgctgagga tcgcgtcagt cacgttcgcg ttatccagtt ggcaaacgcc agtttttaga
     8821 ggattaaata acggatcggg actggacgta tctgcctggg tatgtacggt tattgcacag
     8881 tcaggtgcca gcccggcggc gaaggcttcg cctgttttac gggtacgctc gtcgacatca
     8941 gcaataatcg cgacctgacc agactgcggg cagccctttt tcgccagcaa tccgtcggct
     9001 accagacgct ggcgttggta atgtccgaga taggcgatta gctcaccacc gcgcggtgtc
     9061 agccaaccca gttttaccgg ccaggttggc catgcgtctg gggtgacatc ctgcatcagt
     9121 tgcgtggcct tggttggagc acgcacacca tgacgactga caatcaccac actttccagc
     9181 ttcagctccg gctcactctg agcgaatgca gattgcgggg ttaacggaat cagaagagat
     9241 aaaaatggga ttaagatcgc tttcatcgat atgtttccgc ttttcatatc tgacattatt
     9301 gattgcctga tgcgatgcta atgcgccgca tcaggcctac gttcgttaac ttttcagacg
     9361 cgggtccagc cataccaaca caagggtgga gagcgaacac atcaacaaaa tgccgacgaa
     9421 ccaaagtaaa taccacattg ttcctgctcc ttagtacaac tcgttttcgt tacggcggag
     9481 agtttctgtt gtcatgcgtc cccacatttt gtagtagctc cagagagtgt agagcaacac
     9541 aatgggcaaa aatatcagca cgattaccaa cataatgctc agcgtcagct gactggaagt
     9601 gctgtcccac aacgtcaggc tggagattgg actcacgctt gacggcatga caaaggggaa
     9661 cagcgtgatg cctgccgtga aaatcacgcc aaattgcatc aatgacgcca ttaaaaatcc
     9721 ccaacccggg cgaccacgat aaatcgccat caccgtcagc aatgggcaga agaaccccag
     9781 cagcgggaag atccacaaaa cgggcgattc gacgaaatta ttcatccagg caccaggtag
     9841 cactgccacc agtttcatta acggattgga aggaccgtta gcatcctggg cgagcagtac
     9901 aaagccatca ataccgaccc acagccagta acccgccagc aaaaagcaca gcatcaccaa
     9961 cagtgcagcg cgtttggtgg ccagctgtga acgcagatga atcacaccaa cagttttcag
    10021 ttgtaaccag acgccacctt gcaaaatcac catcccaagg ctgagcaatc cgcacaataa
    10081 agggaatggc gtcagcagtt gccagaagct gccaagatac tccacgcgta attgcggtgt
    10141 gaaggcaaac ggcacgccga gcaacaagtt gccgaaggcg ataccgaaga ctaccggcgg
    10201 caccagactg ccgatgacca gaccggcgtc ccacatttta cgccagcgtg catcggcgat
    10261 ttttccgcga taatcaaagg ccagcgggcg gaagaacagt gagcacagca ccaggatcat
    10321 cgccacataa aagccggaaa acgccgctgc atacactctg ggccaggcgg caaataatgc
    10381 cccaccagcg aggatcaacc agacctggtt gccttcccag tgtgcaccaa cgctgtttat
    10441 cactatccgg cgttcatcat cattacgcgc caccagcggc agcagacagc cgatccccat
    10501 gtcaaatccg tcggagatca taaagaccac caggatcacg ccaatcagca gccaccagat
    10561 gaagcgcaat gtttcataat caaacatgat tttctccttt acccctgttg ctgcgtcggt
    10621 tgttcactct gcatcgcgct cggccccaga cgggcatatt tctgcatcag gtagacttcg
    10681 gcgattaaga acagggtgta aagccctacg atcatgatca gtgagaaagc cagttgtcct
    10741 gtggttaaag cggagtgcgc ggagtatgtc ggtaagatgt cctgtatcgc ccacggctga
    10801 cgaccaaact cggtcataaa ccacccggct tcaatcgcaa tccacggcaa cggcaaactc
    10861 cagagcgcca ttttcagcac ccagcgatgc tggtcgattt tgccacgcag cgtctggaca
    10921 agcgcaatca gcatcaccag tagcagcagg gaaccacagc ccaccatgat gcggaaactc
    10981 cagaataccg gcgcaacctg aggtatcgcg ccacgcatcg ccgcctggta ctgtgcggct
    11041 gtgacatgat tcatatccgg cgcatagcgg gagagcaaca tgccgtagcc caggtcacct
    11101 tccagttccc ggaatgcctg caacacatgc ggctcacgat tgccttgcga tatttcctgc
    11161 attaacagcc aggccatacg tccgcgttgc aagcgtgggt aggtttcagc catcaaattc
    11221 ttcagacccg gcacgggttt atctaatgag tgagtggcga ggatccctag cagcgcggga
    11281 attttgatgg caaaggcgtt acgctcttga tcctgttccg gccaggcaac cacatggaac
    11341 ggtgcaggtg caggttccgt ttgccactcc ccttccatcg ccgccagttt taccggttgt
    11401 acttgcgcga cttcatacgc agaactgtct ccgagttgca gggtaccgat aatcgccaga
    11461 gtaccgaaga cggaaccgat ggcaaacgaa cgtaatgcga catcgcgctc ccgtccgcgc
    11521 agtaaatacc aggcgctgat cgccataata aacatggccc cggtcacgta gcccgccatt
    11581 acggtgtgca caaatttcac ctggctgacc gggttaaaga ccagctcgct gaagctggtc
    11641 atctccatac gcagggtgtc gatatcaaaa tgcgcaccgg tcgggtattg catccaaccg
    11701 ttggcattca atatccacaa tgcagagaga tttgaaccga acgccaccag ccacgtcacc
    11761 agcaggtgct ggtatttatt cagccgctgc cagccgaaga agaacagccc gacaaaggtg
    11821 gattcgagga agaaggccat taatgcttcc atcgccagtg gtgcgccaaa aatatcgccc
    11881 acatagttgg aatagaatga ccagttagta ccaaactgaa actccatggt caggccggta
    11941 gccacgccaa gagcaaaatt gataccgaag agcttacccc agaagcgcgt catatcgcgg
    12001 tagattgttt tgccggtgac cacgtaaatg gtttccatga tagccagcaa aaaaatcagc
    12061 cccagggtaa ggggtacaaa taaaaagtga tacagcgcgg tcagagcaaa ctgccagcgc
    12121 gataaatcaa tgacatccca catgcgcact cctgtaggcg aagagagaaa aataacccga
    12181 taatggtaga tctccctctt tatcctgaaa ctctacgacc agaggtattc gccgattgcg
    12241 atattcataa gactgaaagc gatacttacc gtctttttac gtcggtgcag cttccgccag
    12301 ccactgacat acctcgctaa gccgctgcgc agagtcgacc aaatcttctg gcgctgccag
    12361 caccacttcc ggtattgggc agatttcata actttccagt aacgggcctt ttaaggtgtc
    12421 cgtacagcgt aaatgccaga catggcgtaa ccccgtggcg ttgatatagc tctcgccata
    12481 gccaatggta cgaatctgaa tatttcccgg cccacagaga cgtgagagaa aaagccgatc
    12541 agcctcgctg atgggtaatt gcgtcagatt aatgctgtgc ggctgcgcgt cggggttacg
    12601 tacatgtgcc agtaactcat gcgccaacgg taggccattc atcaggccat caatgggcgg
    12661 cggtaacagc gaatctgtcg gcaagagatt ttgcgttgcc gcctgccaca acgccagcgg
    12721 cgcgcagcca gcctccagtt tgtcctccag caacttttcg ccgcgacgtc tgcgcacccg
    12781 ccataatccg cagaagatcg cctcctgtat ttcactttca ctgtcgtcag cctgctgaat
    12841 gcgcactgac acttcccctt ccccgagaag cgtattgata aagtgacgat cgtcggcgtt
    12901 gagatttaac agatcgactg tcagcacctc gcccaacggc ggttggcgtt cgcttagttg
    12961 atgttgtaac tcgtttaaca gcgcaatcac ctgcgggctg tgagcacatt gctccagggc
    13021 cgccatactc ggttcatcat tcacctgaca ggtgatcggc agtggattca tgctgaaact
    13081 gtcatcgttc ggttgcgttc ctggccccag cagatggaaa aaagtttcgc tcatgaggca
    13141 cgctcctgct gcgattcgac aagcccgcgc atcaggttta tcagttccgc ccacgggtga
    13201 ataccattca gcacgccgcg atagtttccg ccggtaaaca ctaaagtggc aggaaagcga
    13261 aagacgccaa agcgatcgcc gatggcttcg ctctgctcaa ggtcagcaat cgccacctgc
    13321 catgtatagt cgggaaactc gcgcagtaat tcgccaatca ttaccggatt atcgctgacc
    13381 tctggcgtgc gtttcgggtc actgcttaat aacaccacgc cgtctggcgc ttgcgtaagc
    13441 cagtcgtcaa gacgggattc actgactggc gtccagccgc gcgccagcat tcgttgccac
    13501 aacgcatcaa atggcgtgtc gttgctcatc cctgctcctc ctgtgtcatc cggtactggc
    13561 gcaagcgaac gccttcgtaa ttttccatcg acagacagtc ataattgaga cagcgcgatt
    13621 cattagccgg ttgcggcaca attccccacg cagccagctg cgccagcgcc gcctgttccg
    13681 cagcgggcag ttgctcccgt gccagttcgc tcaggctacc gccgtagtcg tcgagcattg
    13741 cgggttgcag accgacgagg gcaatatgtg ctggcagatg tccgcggata tccgccagcg
    13801 ccaacacttc ggagaaactg ttctgatgca ggctcatttt cttcgcgctg agataagccg
    13861 gaatgcgttc tccggcatag gttcgcagcg ttccaggttc cagcccgtag tcaatggcat
    13921 cgagaatcaa cagatggctg gcgctttcga cataccccag caagttcagt ccctgagtac
    13981 cgccatcgac aatctccaca tactcgggcc agtggtaatg ggcatacagc cgttccgcca
    14041 cccgcacgcc gaagccttca tcggcccaca gcaggttgcc cagccccatg accaccacgc
    14101 gttgctcgct catgaacgct ccttgttact tattttgcca aatttgtggc tacggtagcc
    14161 gttgaccatg gtggagatca ccgtgtcgtc ggacatgatg tcttcacgca gtgccatgta
    14221 gacatgaccg atcacaaacg cgccaatcag ccacatcccc agccgatgcc agctgtgaat
    14281 gtccattgag ttgccacccg tccagtagaa aaattccacc acataacgga acggcgcaaa
    14341 aatagcgtac tggctgtgtt cgctgtacag cgcaaaacca gtgatgatca taaagaccga
    14401 catcaggaaa tagccgaaca tcgccgcctg ggcgatggga ttatggccta tatcggcact
    14461 cggacgtttt gccagaaaca gataccagcg gatttcatac cacacgccct gccaccagct
    14521 tttacgccat accggcacga taaacagctc gcgggagtat cgattgccaa caaaagccca
    14581 gtagatccgc atcagcaaaa ccacggtaaa aaccatcccg gcgctgaagt gaattaacct
    14641 gatgtagccc atatagaaca gatacgtcgc ctcgccgctg acggaaggta gcggcttgcc
    14701 gataaagtat ccggtgacca tcaacaccgc catgcataac accgtcaacc agtgccagat
    14761 gcggactggc gcttcaaaga catagtggct gacaacgttg tcgcttttct gttgcatgat
    14821 gattctcctt cgctgttaac gcacctgcac ggagatcagc tcgctaccgt cgtcgcccag
    14881 cacgtgtgtt gaacaggcga ggcacgggtc aaagctgtgc agagtacgca ggatctccag
    14941 cggttgctcg gggatcgcca ttttggtgtt catcagcgcc gcttcataag cgccaatctg
    15001 ccctttggga tcgcgcgggc tggcgttcca ggtggtcggc accacgcact ggtagagatc
    15061 aatcttgcca tcgcgaatgg cggcccagtg gcctaacgcc ccgcgcggcg cttcggtaaa
    15121 accgacacca cggcactctg tcggccaggt tgcaggttcc catttttccg tggaagcagt
    15181 ggcgagattg ccgtttttca ggttggtcat cagcttgtcg aagaaatact gcaacttacc
    15241 tgcggcccac tgcgcttcgt gcgcgcggca caaaatgcgg cctaacgttg actggatacc
    15301 ggaaagcggc aggttcaacg ccgacatcat gcgatcgacc gactcaacgg tcgcagcatc
    15361 gcctttgtga taagcgatta acgtgcgtgc cagcggcccc acttccatcg cgttaccgcg
    15421 ccagcgtggc gctttgatcc acgagtagcg ttcctgttca ttcagctgct gaatgttggt
    15481 atcgctgcct ttgacatcgc cggggttgta ccacgggtcg gtaatgccat cgaatggatg
    15541 acgcccgacc tgatcgttgg gataacgata ccaggcgtga tcgacaaact cctgcacctg
    15601 ctgcggatca accaaatcca ctggcagcac attgttgaag tcgccgttaa tcaccgcgcc
    15661 gccaggcatc agcagacttt tctcaccaaa gtcgttggca atatccggga atgcgccgta
    15721 gctgaggaca catttatcag aaagacccgt gccgatttcg ctccacggtt tgttgaactg
    15781 accgatggct aaggcgtcgg ggatcatcac gttgttaatg aagtctgccg tgcgggtgat
    15841 aatcgactgc accaggttca ggcgttccat attgaccgcc ccgaccgcgc cgctttcgtc
    15901 aatgttaatg gcgcaaggca tcccgccgac aatccagttt ggatgcgggt ttttaccgcc
    15961 aaagaccgcg tggattttca caatttcacg ctggaaatcg agagcttcga gatagtgggc
    16021 aaagcccatc aggttagctt ctggcggcag tttgtactgc gggtgccccc agtagccatt
    16081 gcggaagatc cccaactgcc cgccttcaac aaattttttc aggcggtttt gtacgtcgaa
    16141 gaaatagcca ggggatgatt tcggccaaga ggagagactt tgcgccagtt cggaggtttt
    16201 ccgcgggtcg gctttcagcg catctaacac atcgatccag tccatcccgg caagctgata
    16261 gaagtgcacc agatgatcgt ggcaccagag cgttgccagc ataatgttgc ggatgatatt
    16321 ggcgttgtcc ggcactttaa taccgatagc atcttcgatg gcgtaaaccg aagccagggc
    16381 gtgtacgcca gtacagacgc cgcagatacg ttcaacgaac gcccacgcat cgcgcgggtc
    16441 gcgcccttgc agaatgatct ccagcccgcg aaacatggtg ccgcaggaga cggcattggt
    16501 gatcacattc tgatcgttaa tattcacttc gcagcgcatg tggccttcga tgcgcgtaat
    16561 cgggtcgacc accaggcggc gtccggcatt attgatggtg tatccctgag tttcgtactg
    16621 agtgctcatg cctgtttatc ctcattgcct ggctgatgtt cggtttctgt aggttgctgg
    16681 ttatgacgtc tgcgctggtc aacggcgctg gcgactgcgt gcacaccaac agccgctgcc
    16741 accacgccaa gcgcggttaa accgacggta tcggcggtgg aatgagtacc catttgcgga
    16801 atatcgacca cgcggctgta gaacgaaccg cgatcccaga aaccattttc cgcacagccc
    16861 aggcagccgt gaccagactg gattgggaaa gaaacgccat cattccagcg tgtggaggaa
    16921 caggcgttat aggtggtagg ccctttgcag cccattttgt acaggcagta acctttgcgg
    16981 gcagcgtcat catcccaact ctggacgaac tctccggcgt cgaagtgggc gcggcgatag
    17041 catttatcgt ggattcgctg accatagaac atcagcggac ggcccattct gtcgacatct
    17101 ggcaagcgat caaaggtcac catgtaagta atgatggcgc tcatcacatc cgggatcggc
    17161 gggcagccag gtactttgat aatgggtttg tcggtgatga ctttgtcgat aggcgttgcc
    17221 tgcgtcggat tgggccgcgc ggcctgcacg cagccccagg acgcgcaggt tccccaggcg
    17281 ataatcgcgc tggctccggc agcggcacgt ttgagtttct caataaacgg tcgaccgctg
    17341 ctgatacaga acatcccctg ctcgcccagc ggcggattac cttctactgc gaggatatat
    17401 ttgccattgt attgcgtgat gatgtcttcg aagacttctt ccgcctgggt tccggcggca
    17461 gccatcaaag tatcgtcata atcgagggaa atcagggaaa ggatgacgtc cttcgccagt
    17521 gggtgagcgg agcggataaa agattcggta cagcaggtgc attccagacc gtggatccat
    17581 accaccggaa tgcggggttt gttctccagc gcccaggcaa tctttggtgc cattcccgcg
    17641 cctaatccca gcgacgtggc agccagacta caatatttga gaaagctgcg ccgggtaacg
    17701 ccctgacgcc gcatggcctg gtaaaatgtt tcctcgttat tcatatcgca cgtctcttct
    17761 ccttgcgaca ccggcaggac accgggcgct tatggtcggc aagcgacgtc cctgtagcaa
    17821 ggcaaaccat cgcttcacgt gagatgctga aaacgaaagc tcatccttct gcactggcac
    17881 acgtcgccag aaagtattgt taataaagcg tagtgaaact tttgcacaaa acaatacaaa
    17941 ctgtgtggat ttatctttta gcgataaaaa tggacctatt tttcttttgg ccgggcggtg
    18001 gggatgttta gccggttgct aaacgagtaa aagagaagga attcatggtg atgaaatgat
    18061 caatctgctg ttatttcacg ctatcaatgc acttttggct gtttttcagg caaacaaaca
    18121 aattaggggt ttacacgccg catcgggatg tttatagtgc gcgtcattcc ggaagtgtgg
    18181 ccgagcggtt gaaggcaccg gtcttgaaaa ccggcgaccc gaaagggttc cagagttcga
    18241 atctctgcgc ttccgccaaa taagataagg ggttagctaa atgctaaccc ctttttcttt
    18301 tgcctgtcga aattctcagg gcgttatatt tgcttaatga cctgataatc cgctgttaaa
    18361 cctgttccag atcaaatgcg taaagatggg taaaacttct gggtgccctt acgcattatc
    18421 attatgctgc ttaattaatt acatctgtca tagagagtga ctcaatggat cgtattgtta
    18481 gttcttcaca tgaccgtaca tcactgctta gcacccataa ggtgctgcgt aatacctatt
    18541 ttctgctgag cctgacgctg gccttttcgg cgattaccgc aactgccagt acggtgctga
    18601 tgctgccatc tccgggtctg attctgacgc tggtgggtat gtatggtttg atgttcctga
    18661 cctataaaac ggcgaataag ccgaccggga ttatctccgc attcgccttt accggttttc
    18721 tgggttatat cctcggacct attctgaaca cctatctgtc tgccggaatg ggtgacgtaa
    18781 tcgctatggc actgggcgga acggcgttag tgttcttctg ctgctctgca tatgtgctga
    18841 ccacccgcaa agatatgtcg ttcctcggcg gtatgctgat ggcgggtatt gtggtggtgc
    18901 tgattggtat ggttgctaat atcttcctgc aactgcctgc tctgcatctg gcgatcagcg
    18961 cggtcttcat tctgatctcc tctggcgcta tcttgtttga aaccagcaac atcattcatg
    19021 gcggtgagac gaactatatt cgtgccacgg ttagcctgta tgtttcgctg tacaacatct
    19081 tcgtcagcct gctgagcatt ctgggcttcg ctagccgcga ttaatctcac ccgctaacac
    19141 tttcatagcc tcgctttatg cggggctttg ttttttgtta cactgccgcc agtctgatta
    19201 aggtagtaat aattatgctg atcttcgaag gtaaagagat agaaacggat accgaaggct
    19261 atctcaaaga aagcagccag tggagtgagc cgctggctgt ggtgattgca gagaacgaag
    19321 ggatttcgct gtcgccagaa cactgggaag tggtgcgttt tgtgcgtgat ttctatctgg
    19381 aattcaatac ttctccggcg attcgtatgc tggtaaaagc gatagcgaat aaatttggcg
    19441 aagaaaaagg taacagccgc tatttatatc gactgtttcc gaaaggtccg gctaagcaag
    19501 ccaccaaaat tgctggcctg ccaaaaccgg taaaatgtat ttaacggata cgaaaatcgg
    19561 ctaattcccc cgagggatga tgcggctcgc taagcacccg ttccacacgc gcagaacgcg
    19621 ggccgccact ctttaaccac tgcattaatt tttccacctg cccttcttca ccgcaggcaa
    19681 ccacttccac gctgccatcg tcaagatttt tggcgtaccc ggttaaaccc agtcttttcg
    19741 cttcatactg tgtggtgtag cgaaatccta cgccctgaac tcgcccgtaa acccaggcaa
    19801 ttatgcagac tttcgacatt gctgctctcc ttatccttaa tggacgcgtt gcaaatccat
    19861 ggaaaactcc ggacaatgac gcccatttct ttgcatcgac agaaaagtaa attatgagtg
    19921 tacgtttagt gttagccaaa gggcgcgaaa aatcattact tcgtcgccat ccgtgggtct
    19981 tttccggggc cgttgcccgc atggaaggta aagccagcct cggtgaaacc atcgatattg
    20041 ttgatcatca gggaaaatgg ttagcacgcg gcgcttattc accagcttcg caaatccggg
    20101 cgcgcgtctg gacgtttgac ccgtctgagt ctatcgacat tgcttttttt tcccgccgtt
    20161 tgcaacaagc acaaaaatgg cgtgactggc tggcgcaaaa agatggcctc gacagctatc
    20221 gtttaatcgc cggagaatct gatggcctgc cgggtattac tatcgatcgt ttcggtaatt
    20281 ttctggtgct gcaactgctg agtgctggcg cagaatatca gcgcgcggca ttaattagtg
    20341 ccctgcaaac gctgtacccg gaatgtgcga tttacgatcg cagcgatgtt gcggtacgta
    20401 aaaaagaagg gatggagctg acccagggcc tcgtcaccgg cgagttgcca cctgccctgc
    20461 tgccgattga agaacacggc atgaaactgc tggtggatat tcagcacgga cacaaaacgg
    20521 gctactacct ggaccagcgt gatagccgcc tggctacccg ccgctacgtt gaaaataaac
    20581 gtgtgctgaa ctgtttctcc tataccggtg gtttcgccgt atcggcactg atgggcggtt
    20641 gcagccaggt tgtcagcgtt gatacctccc aggaagcgct ggatattgca cggcagaacg
    20701 ttgagctgaa caaactggat ctgagcaagg ctgagtttgt ccgtgatgat gtctttaaat
    20761 tgctgcgtac ttatcgcgat cgcggtgaaa aatttgacgt tatcgtgatg gacccgccga
    20821 agtttgttga gaataaaagc cagttgatgg gcgcgtgtcg tggctataaa gatatcaaca
    20881 tgctggcgat tcagttgctg aatgaaggcg gtattctcct gactttctcc tgttccggtc
    20941 tgatgaccag cgatttattt cagaaaatca tcgcggatgc cgcaattgat gccggccgtg
    21001 atgtacaatt tatagagcag ttccgtcagg cagccgatca tccggtgatc gctacctacc
    21061 cggaagggct atatctgaaa gggtttgcct gtcgcgtcat gtaacttgaa atgtggaatt
    21121 ttacccttat atcaaaggta aagatttccc gggaggtgac tatgattgcc agcaaattcg
    21181 gtatcggcca gcaggtccgc cattccctgt taggttatct cggagtggtc gtggatatcg
    21241 acccggttta ttcgctctct gaaccgtcgc ctgatgagct ggcggtgaat gacgagcttc
    21301 gcgccgctcc gtggtatcac gtggtgatgg aggacgataa cggcctaccg gttcatacct
    21361 acctggctga agcccagcta agtagcgaat tgcaggatga acatcctgaa cagccatcca
    21421 tggatgagct ggcgcagacc atccgcaaac aactccaggc cccgcgtctg cgtaactaat
    21481 ccctgaacga atccccggaa ggtttttgcc ttccggggat tttttatttg gccaggccca
    21541 ggcgagggat ctcaatagcc gggcagcgat ccatcaccac atttaacccg gcatcccgtg
    21601 ccagtactgc cgcttgctca ttaatcaccc ccagttgcat ccagagggtt ttcgcgccaa
    21661 tggcaatagc ttcctgtgcc acgccccacg ccgcttctga attacggaaa acatcgacca
    21721 tatcgacctt ttccggaacg tctgccagcg tgccataccc ctgctggccc agcagcgttt
    21781 tgccggcaac ttttggcgaa accggaatga cgtgataccc ctggtcgagt agatatttca
    21841 tcacgcgata gctgggacga tcgggtttgt cgcttgcccc caccagcgca atggtgtgag
    21901 tggacgttaa aatgcctgca atatcggttt ctttcattat ttcctccaga ctgtttaaca
    21961 aagtgtacga caaagccgac atgccaacca tgcagctaat acgaatgaat gagagcaaaa
    22021 gcgggaaata tgtctatatg ttagtaagta tgattaaaga aaaattttga cacattatta
    22081 ttcgggcggt cttttgacag gagctaccta tgaaaaccgg catcgtgacc accttgattg
    22141 cattatgttt gccggtatcc gtttttgcca ccacgctgcg gctttcaacc gatgtcgatc
    22201 ttctggtact cgacggcaag aaggtttcca gctctctgct gcgcggtgcg gacagcattg
    22261 aactggataa tggaccgcat cagttagtgt ttcgcgttga gaagacgatt catctttcca
    22321 atagtgaaga acggctgtat atctccccac cgctggtggt cagttttaat acccagctca
    22381 tcaaccaggt caattttcgc ctgcctcgcc tggagaatga gcgggaagct aaccattttg
    22441 atgccgcgcc gcgccttgaa ttgttggatg gcgatgcgac gccgattccg gtaaagctgg
    22501 atattctcgc cattacctca acggcaaaaa cgattgatta tgaggtcgag gttgaacgct
    22561 ataacaaatc cgcgaaacgc gcttcactac cgcaatttgc cacgatgatg gcagatgaca
    22621 gtacgctgct ctcgggtgtt tccgagctgg atgctattcc accgcagtct caggtgctca
    22681 cagaacaacg gctgaagtat tggtttaaac tggctgaccc acaaacgcga aatactttcc
    22741 tgcagtgggc ggaaaaacaa ccatcttcct gagatttttg tgcctgtgcg caggcttttt
    22801 cggtctttat cttgcagcga taagtgctta cagtaatctg taggaaagtt aactacggat
    22861 gtacattatg gaactgacga ctcgcacttt acctgcgcgg aaacatattg cgctggtggc
    22921 acacgatcac tgcaaacaaa tgctgatgag ctgggtggaa cggcatcaac cgttactgga
    22981 acaacacgta ctgtatgcaa caggcactac cggtaactta atttcccgcg cgaccggcat
    23041 gaacgtcaac gcgatgttga gtggcccaat ggggggtgac cagcaggttg gcgcattgat
    23101 ctcagaaggg aaaattgatg tattgatttt cttctgggat ccactaaatg ccgtgccgca
    23161 cgatcctgac gtgaaagcct tgctgcgtct ggcgacggta tggaacattc cggtcgccac
    23221 caacgtggca acggcagact tcataatcca gtcgccgcat ttcaacgacg cggtcgatat
    23281 tctgatcccc gattatcagc gttatctcgc ggaccgtctg aagtaatatt gcacaggtgg
    23341 caaacgccac ctgtttctta cggttttctc gccaccggca catccagatt tttcagtatt
    23401 tccacaaagg gagagggatt ctctttgtta aacagtgccc atacccgatg gcgtgcccgg
    23461 gtcagcgcca cgtacattaa ccgccgttct tcagcgtccg ggaaatcctc aaccggtggc
    23521 agtagcgcct cttccataat cgactcccgc gccgcagccg gaaaaccatc acttccctcc
    23581 tgcaagccaa cgatgatgac gtaatccgcc tgttgccctt tgctggcatg aatggtcata
    23641 aagtcgattt gcaacttcgg ccagcgtgtt gccgcttttt ccaggctggc aggcctcatg
    23701 tgatggtaac gcgccaggat aagaatgcgc tcttccggtt tggcataacc agagagctta
    23761 tccagcaaag cgtcaagttg actctcatcc aataacgtga cggctttttc gtctccattg
    23821 gttaagctgt ttagcggctt tttcaactgg cctgggttct gctgaataaa ccggtttgcc
    23881 acctcaccga tacgactgtt aaaacggtaa gtcgtgtcta aatcacagcg atcgccttca
    23941 ccaaagtttt catggaaagc ggtggtgagc gacatttgcg caccgctgaa tcggtaaatc
    24001 gcctgccagt catcaccaac ggcgaacaac gtcgtctgac tgttttgctt gcgtaatgcc
    24061 gctaacaacg ctgcccgctg cggcgagata tcctgaaatt catcaaccag aatatgcttc
    24121 cacgggctga taaagcgacc tttctccaga atcacaatcg cctgatgaat aaggcccgaa
    24181 aaatcgacag cgttttctgc cttcagcgca cctttccagg cttttaataa cggggccatc
    24241 aacttgatac gtttactgaa cagatcgcga atctcttcgg gtgcactggc aatcatttct
    24301 gcctgtgcac caccgtgcat ccgcatcaga cttacccaac gatcgaggcg tgaggcaaga
    24361 cgacgctgta atttttcatc atcccagaag ttaccttctg gcactgacca ctgcatttct
    24421 tccgtcagcc attgccgcca gcccttcgcc tgcgctttct tttcgctgca ttgcttgcgc
    24481 cactcagcaa taaagagttc atgacgggca gcggtatcat tttccagttt gctgactatc
    24541 ggaacttttt tgctgccctg ctgaataata tgcagcgcca gcgcatgaaa cgtgcgtgcg
    24601 gtaatgtctt cggtatgtag ccgttcgcga atacgctcgt ccatctcttc agcggctttg
    24661 cgaccaaacg ccagcaataa aatttgctca ggggacgctt caccacgcgc cagcaaccag
    24721 cctgcacggg ccaccagcac cgacgttttt ccgcttcctg cacctgccag cactaacaga
    24781 gaatgctcgc cattaacgac tgcccgggcc tgcgccggat tcagcggtga agactcgacc
    24841 tggcggaaaa aatccgcata ctcggtaagc atggcttcgg tatacgcctg gttatgctgc
    24901 aaccgagcgc tttcaatatc tttcaaccag gcctgacatt tacgccacgc ctcacggcag
    24961 ttatcgaatt cttccagtcg gttaaccggc aacggcaacg ccgacatagc ctggcggatt
    25021 tgttgctgaa cgccagaggt ttgctcacgc gtcagccatt tattttctcc agtgcgcgtg
    25081 gcaatcaaat ccagttgctg gcgtaaaaca ccagacgcaa tttcgctcat ctcgccactc
    25141 caccgccgcc agtgagcatc aagatgatgg taaaaacgct gcgtctcgcc ccattcggtg
    25201 ccgtgcagac gcaccacttt ttcgtccggc agtacaaatt ccagctcgcc ccataccaga
    25261 ccgcgcttac agtgaatcgc cagtaattga ttgaaaggaa taaggtattc gtggcgatcg
    25321 ccggagactt taatcccggc attgaggatc accgcccgat cgtaagggtg ctgtgccaga
    25381 cgttttccaa gcgttgtcgc tttcagttcc atgactctgt agatccacac gaaattattt
    25441 cttatcagtg taaccgtcag agaaaacgtg ctccagccca aaaaaacgtt acaattgccg
    25501 cccattactg aaaaccacag taaagcgagg ttttatgcgt accgttttga acattctgaa
    25561 ctttgtgctt ggcggatttg ccaccactct gggctggctg ttggcgactc tggtcagtat
    25621 tgtgctgatt tttaccttac cgctgacacg atcctgctgg gagatcacta aactgtctct
    25681 ggtgccttat ggcaatgaag ctattcatgt cgatgaactg aacccggctg gcaaaaatgt
    25741 gctgctgaat actggcggta cggtattgaa tattttctgg ctgattttct ttggctggtg
    25801 gttatgcctg atgcacattg caacgggcat cgcacaatgt atttcaatca ttggcattcc
    25861 tgtcggcatt gcgaacttta aaattgccgc tattgcacta tggccggttg gtcgtcgcgt
    25921 ggtatcggta gaaacagcgc aagctgcgcg tgaagccaat gcacgtcgtc gttttgaata
    25981 atccactaat atggccttta tgctaagtcc tttgctcaaa cgctatacct ggaacagcgc
    26041 ctggctgtat tacgcgcgta tttttattgc gctttgtgga accacagcgt ttccgtggtg
    26101 gctgggtgat gtaaaactga cgattccgct aacgctgggg atggtggcag cggcgctgac
    26161 cgatctcgat gaccgactgg cgggacgttt gcgtaacctc atcattacgc tgttctgctt
    26221 ttttatcgcc tcggcctcag tagaattgct gtttccctgg ccctggctat ttgcgattgg
    26281 cttaacgctc tctaccagcg gcttcatttt gctcggcggt ctgggtcaac gctatgcaac
    26341 aattgccttc ggtgcattgc tgatcgccat ttacactatg ttgggaacat cactgtatga
    26401 gcactggtat cagcagccga tgtatctgct ggccggtgcc gtctggtaca acgtcctgac
    26461 acttattggt catctgctgt tcccggtccg cccgctgcag gacaacctgg cgcgttgcta
    26521 tgaacaactg gcgcgttatc ttgagctcaa gtcgcgcatg tttgatcctg atattgaaga
    26581 tgaaagccag gcaccgctgt acgatttggc tctcgccaac ggacaactga tggcgacatt
    26641 gaatcagacg aaactctcgc tgctgacccg cttacgtggc gatcgtggtc aacggggaac
    26701 gcgtcgcacg ctgcattatt actttgtcgc gcaggatatt cacgagcgtg ccagctcttc
    26761 tcatattcag tatcaaacat tgcgtgaaca ttttcgccac agcgacgtgc tgttccgttt
    26821 tcagcggctg atgtcgatgc agggccaggc gtgccagcaa ctgtcacgct gtattttgct
    26881 gcgtcagcct tatcaacatg atccgcattt tgagcgcgct tttacgcata ttgatgctgc
    26941 gctggagcgg atgcgcgata acggcgcacc cgccgattta ctcaaaacac tgggattttt
    27001 gctgaacaat ttacgcgcca ttgatgccca actggcaaca attgaatcag aacaggccca
    27061 ggcactaccc cataataatg acgaaaatga gctcgctgat gacagcccgc acgggttgag
    27121 tgatatctgg ctgcgtctta gccgtcactt cacgccggaa tccgccctct tccgtcatgc
    27181 ggtaagaatg tcgctggtgt tgtgcttcgg ctacgccatc attcaaataa ccggaatgca
    27241 tcacgggtat tggatcttgc tgacaagttt gtttgtctgc cagccaaact ataacgccac
    27301 gcgccaccgc ctgaagttaa ggattattgg tacgctggta ggtatcgcca ttggcattcc
    27361 tgtgctgtgg tttgtgccat cactggaagg gcagctggtg ctgctggtta ttaccggcgt
    27421 gctctttttt gccttccgta acgtgcaata cgctcatgca acgatgttca tcacactttt
    27481 ggtgctactg tgttttaact tactgggtga aggttttgaa gtagcgttac ctcgcgtaat
    27541 cgatacgctg attggttgtg ccattgcgtg ggcggcagtg agctacatct ggcctgactg
    27601 gcagtttcgc aatctgccgc gcatgctcga acgcgccaca gaggccaact gtcggtatct
    27661 cgatgccata ctggagcaat accatcaggg gcgtgataac cgtctggcgt atcgtattgc
    27721 ccgccgcgat gcacacaacc gtgatgctga gctggcgtcg gtggtatcaa atatgtccag
    27781 cgagccgaac gttaccccgc aaattcgcga agccgcgttt cggttgctgt gccttaacca
    27841 tacgtttacc agctatatct cagccctcgg tgctcaccgg gagcagttaa ctaatcctga
    27901 aattctggcg tttcttgatg acgcagtttg ctatgttgat gacgcgttac atcatcaacc
    27961 tgctgatgaa gaacgcgtca atgaggcatt agctagcctg aaacagcgga tgcagcaact
    28021 tgaaccacgg gcagacagca aagaacctct ggtcgtacaa caagttggat tattgattgc
    28081 attactgcct gagattggtc gtctgcaacg ccagattact caagttccgc aggaaactcc
    28141 tgtttcggcg taagagagtc agcccattct gcaagctcct ggcggcgtgc caccgggagc
    28201 gcagcttcat gaatgccgat aatggcacct tcaagcataa acagaatctt ttctgtcacc
    28261 agactgtttt gctgccgcag tcgcaaccaa cacatttttg ccccaagtat acgtaacgcc
    28321 cgtacatcct taatccccac ctccccgaga atcgcttcca gatgaaaaga catattgggc
    28381 aaatctttca gtctttcccg ggtattgcgc gtgcttttct ctttcagcgc tgcatcgaga
    28441 gaatatttcg acagacgcac cagcttcagt tgatttcgcc atagactttc atcaacccga
    28501 tagtaattga gggtaacgga tcggccacac tttttatatg tcagccagac aggcggatgt
    28561 tttacacagt actgtgcact ttgctcacaa gcccgaagat acaactcacc atcagaaacc
    28621 atcgcaaaca ccgtgtcgtc aacggtcagg ctgtaactgc caaacaatga tcggtattca
    28681 attgtgccca acgttgccag gtattcttgt gatttataga tccgcttata ggagaggctt
    28741 ttcataaaat tccttttaaa atcataacat aaaagaatga ttcacattaa cggatccgtt
    28801 aactacgaaa ataggcaact tattcttaag gggcaagatt aatttatgtt ttcccgtcac
    28861 caacgacaaa atttgcgagg ctctttccga aaatagggtt gatctttgtt gtcactggat
    28921 gtactgtata tccatacagt aactcacagg ggctggattg attatgtaca cttcaggcta
    28981 tgcacatcgt tcttcgtcgt tctcatccgc agcaagtaaa attgcgcgtg tctctacgga
    29041 aaacactaca gccgggctta tcagtgaagt tgtctatcgc gaagatcagc ccatgatgac
    29101 gcaacttcta ctgttgccat tgttacagca actcggtcag caatcgcgct ggcaactctg
    29161 gttaacaccg caacaaaaac tgagtcggga atgggttcag gcatctgggc tacccttaac
    29221 gaaagtaatg cagattagcc agctctcccc ttgccacact gtggagtcaa tggttcgcgc
    29281 tttacgcacg ggcaattaca gtgtggtgat cggttggttg gcagatgatt tgactgaaga
    29341 agagcatgct gaacttgttg atgcggcaaa tgaaggtaac gctatggggt ttattatgcg
    29401 tccggtaagc gcatcctctc acgccacgag acaactttcc gggctaaaaa ttcactctaa
    29461 tttgtatcat taagtaaatt taggattaat cctggaactt tttttgtcgc ccagccaatg
    29521 ctttcagtcg tgactaattt tccttgcgga ggcttgtctg aagcggtttc cgcgattttc
    29581 ttctgtaaat tgtcgctgac aaaaaagatt aaacatacct tatacaagac ttttttttca
    29641 tatgcctgac ggagttcaca cttgtaagtt ttcaactacg ttgtagactt tacatcgcca
    29701 ggggtgctcg gcataagccg aagatatcgg tagagttaat attgagcaga tcccccggtg
    29761 aaggatttaa ccgtgttatc tcgttggaga tattcatggc gtattttgga tgataacgag
    29821 gcgcaaaaaa tgaaaaagac agctatcgcg attgcagtgg cactggctgg tttcgctacc
    29881 gtagcgcagg ccgctccgaa agataacacc tggtacactg gtgctaaact gggctggtcc
    29941 cagtaccatg acactggttt tattcctaac aatggtccga cccacgaaaa ccaactgggt
    30001 gcaggtgctt ttggtggtta ccaggttaac ccgtatgttg gttttgaaat gggttacgac
    30061 tggttaggtc gtatgccgta caaaggcgac aacatcaacg gtgcatacaa agctcagggc
    30121 gttcaactga ctgctaaact gggttaccca atcactgacg atctggacgt atacactcgt
    30181 ctgggtggta tggtatggcg tgcagacacc aaggctaacg tacctggtgg cgcatccttt
    30241 aaagaccacg acactggcgt ttctccggtt ttcgcaggtg gtgttgagta cgcgatcact
    30301 cctgaaatcg ctacccgtct ggaataccag tggaccaaca acatcggtga cgcaaacacc
    30361 atcggtactc gtccggacaa cggtctgctg agcctgggtg tttcctaccg tttcggtcag
    30421 ggcgaagcag ctccggtagt tgctccggct ccagctccgg caccggaagt acagaccaag
    30481 cacttcactc tgaagtctga cgttctgttc aacttcaaca aagcaaccct gaaaccggaa
    30541 ggtcaggctg ctctggatca gctgtacagc cagctgagca acctggatcc gaaagacggt
    30601 tccgtagttg ttctgggtta cactgaccgc atcggttctg acgcttacaa ccagggtctg
    30661 tccgagcgcc gtgctcagtc tgttgttgat tacctgatct ccaaaggtat cccggcagac
    30721 aagatctccg cacgtggtat gggcgaatcc aacccggtta ctggcaacac ctgtgacaac
    30781 gtgaaacagc gtgctgcact gatcgactgc ctggctccgg atcgtcgcgt agagatcgaa
    30841 gttaaaggca tcaaagacgt tgtaactcag ccgcaggctt aagttctcgt ctggtagaaa
    30901 aaccccgcag ctgcggggtt tttttcgcct ttagtaaatt gaactgactt cgtcagttat
    30961 tccttaccca gcaatgcctg cagatcatgc ttcaaagaag acattttatt cgcgtatttc
    31021 tctttgtttt ccgcatcttc aatcagctga acaatcgttt cagaaagcgt tttaccgcga
    31081 cgctgcgcaa gaccagccag acgttgccag acaataaatt ccagatcgat cgattttttg
    31141 cgcgtatgct ggtgttccgc attaaaatgg cgcttccgtc tggcccgaat ggtttgcttc
    31201 atgcgattga ccagttccgg attcatatgc ttgtcaatcc agccatttac cagcacgggt
    31261 tcattttcca gcgagagcaa cacatcgacg gcttcctggg cggcactggc ttctatgtaa
    31321 cgggtgatta actccccttc gcgatgcttc ttcaccagat acttccattt ccaaccgctt
    31381 tcaagatttt caagttgttg atatttcata gcgatctcaa tgttaccgtg taactcttta
    31441 cagaatatca gctttttagg ccagtgaaga aaagaatctc catcctgtga gctttaacgc
    31501 ccatgccagg agtattgtcg gtgctttcta cgtgtgctgt agtgccggtt acggtataat
    31561 cgcggctttg acaacagact aaaaaacatc aactttgacc attacgaaac ttgcatggcg
    31621 tgacctggtt cctgataccg atagctatca ggaaatattt gctcagccac atttgattga
    31681 cgaaaacgat cctttattca gtgatactca accgcgactg caattcgcac tggagcagtt
    31741 gctgcatacg cgagcatcct cctcttttat gctggcgaag gccccggaag agtctgagta
    31801 tctgaatctt attgccaatg ccgcgcgtac gctacaaagc gatgcaggcc aactggtggg
    31861 cggtcactat gaggtttccg gccactccat ccgcttacgt cacgcagtga gtgcagatga
    31921 taattttgcg actttaacgc aagttgtcgc tgccgactgg gtagaagcgg agcaactctt
    31981 tggctgcctg cgccagttta atggcgacat taccctgcag cctggtctgg tgcatcaggc
    32041 aaatggcggt attctcatca tctctttgcg tacactgctg gcgcaacctc tgctgtggat
    32101 gcggctgaaa aatatcgtta accgcgagcg ttttgactgg gttgcgtttg atgagtcgcg
    32161 ccctctcccc gtctctgtgc cttcgatgcc attgaagctg aaagtcattc tggtaggcga
    32221 acgcgaatca ttggctgatt tccaggagat ggagccagag ctttcagagc aggctattta
    32281 tagcgaattt gaagatactc tgcagattgt cgatgcggag tcagtaaccc agtggtgtcg
    32341 ctgggtgaca tttaccgcca gacataatca cttacctgca ccgggagcgg atgcctggcc
    32401 ggtacttatc cgcgaagcag cacgctacac cggtgaacaa gaaacacttc cgcttagccc
    32461 gcagtggatc ctccgccagt gtaaagaggt cgcctccctg tgtgatggcg acaccttctc
    32521 cggcgagcag ctaaacttaa tgctgcagca gcgtgaatgg cgcgaaggtt tcctcgctga
    32581 acgtatgcag gatgagatcc ttcaggagca aatcctgatt gaaaccgaag gcgaacgcat
    32641 cgggcaaatt aacgcccttt cggtcattga atttccgggt catccacgcg cttttggcga
    32701 accttctcgc attagctgcg ttgtgcatat tggcgatggt gaattcaccg acatcgaacg
    32761 caaagcggag cttggcggca atatccatgc gaaagggatg atgatcatgc aagcgttcct
    32821 gatgtcggaa ctacagcttg agcaacagat ccccttctca gcatcgctga catttgagca
    32881 gtcatacagt gaagttgatg gagatagtgc ctcgatggct gaactctgcg ccctgataag
    32941 cgccctcgcc gatgtgccgg tgaatcagag tatcgctatc acaggttcag tcgatcagtt
    33001 cggtcgcgcc cagccggtcg gtggtttaaa tgagaaaatc gaaggcttct ttgctatttg
    33061 ccagcaacgt gagttaaccg ggaaacaagg tgtcattatc cccacagcta acgttcgcca
    33121 tttaagtctt cacagtgaac tggtgaaagc ggtagaagaa ggcaaattca ccatctgggc
    33181 agtagacgat gtgactgacg cactgccgtt attattaaat ctggtgtggg atggcgaagg
    33241 ccaaacgacg ctgatgcaaa ccatccagga acgtatcgcg caagcatcgc aacaggaagg
    33301 acgtcaccgt tttccatggc cattacgttg gctgaactgg tttattccga actgatcgga
    33361 cttgttcagc gtacacgtgt tagctatcct gcgtgcttca ataaaataag gcttacagag
    33421 aacatggtag ataaacgcga atcctataca aaagaagacc ttcttgcctc tggtcgcggt
    33481 gaactgtttg gcgctaaagg cccgcaattg ccagcaccga acatgctgat gatggaccgt
    33541 gtggtcaaaa tgaccgaaac gggtggtaac ttcgacaaag ggtatgttga agcagaactg
    33601 gatatcaatc cggatctgtg gttcttcgga tgccacttta ttggcgatcc ggttatgccg
    33661 ggatgcctgg gcctggacgc aatgtggcag ctggtagggt tctacctcgg ctggctgggc
    33721 ggcgaaggta aaggccgcgc gctgggcgtt ggcgaagtga aattcactgg tcaggtactg
    33781 ccgacagcga aaaaagtgac ctaccgtatt cactttaaac gcattgttaa ccgtcgtctg
    33841 attatgggcc tggcggatgg cgaagtgctg gttgatggtc gtcttatcta taccgccagc
    33901 gacctgaaag tcggtctgtt ccaggatacg tctgccttct gattataatc agacagagta
    33961 tcaaaaggcg aaacctccgc aatgcggagg tttcttttta aagagacaga atcaggccat
    34021 tactaccctg tccgccatgg cttctcgcca gcctcccagc cattgtgacc tttgattcag
    34081 cgtctgatag ggacacattt cttttgagcg tccggcgatg ccggcctgat aaccacgttg
    34141 atgtgcccgt tccaggcgat ctcgtttttg tctcttcatg cctcgtttcc ctcatactgg
    34201 tttctggtgg aaaagaaaac agtgattact aaatgtgcaa tcacagttaa gcaataccgc
    34261 gaatcataac tgccgtcaat gcgcaaaatt cacgccactg tcatatttgt gagctacacg
    34321 ggcatgtcgg ttgtacaaaa aatgtgaaga ggaacaaatt tttccgcacg ttagaaacaa
    34381 aaaaaccgcc tcaggcaaca taccggagac ggttatattt acaaatcttt ataattaagg
    34441 tagacgcttt atctcttgtg caatagaagc ggcttcctga ctccagacac cggccagcac
    34501 tttaaccatc tcatcgtaac catcctgagt ttgcactcct tccagacgga acggacgttt
    34561 gatcagttgt ccctggtggt tcaacagcca ctcaccactg acaatgacct tgccatcata
    34621 gcgaccgtta aactccgtta cggtaacatt gagcgtgtcc tgggcgcttc ccagaggctg
    34681 ggaggcaacc acccagccgg gcagttgcgt actcaggttg gcaaccaggg tgttgcgcaa
    34741 ctgttgatcc aacgggctgg cccacaagtt gttgttggca atcacatact tcacatcact
    34801 ggtttgataa accacaccat tccccgccag atagtcagga acagtgacct gctctaccca
    34861 taacagacga ttgccctggc tggcggtact ttgtgtaccg ctctgcacca caggtaactg
    34921 gtaatagttt ttattaattt cgccggagct gcatccgacc agccacagtg ctgcaatcgt
    34981 cactagccac tttttcattg tttcgccctc ttcggctctg gatctttttt gtccttcgct
    35041 tcaaatacca gcgcgttact cttctcattg agcgttttca gcaccggttg cagttctcgc
    35101 aacacctgat caaggcgctg catatccgcc accatcttgt tgtaggctgc ggagccaggc
    35161 tggaagccct gcatgctgcg attcaattca cgcaacgttg actgcatatc cgtcggcaac
    35221 tgctgcatcg actggctagc gaggatcttg ttcatgctat ccagcgtcgt ttgcaggttt
    35281 ttcattgtgc gctgactttc agaaagcgta ctggttgcct gttcaatcat cggattcaat
    35341 ggcagtttgt tgatcttatc caacgcttcc atcagtcgtt gctggatttg cgccaggccg
    35401 ccgctaacgg tcgggataat ctgataacca ttaaattcac gaataccggt tattgcaggc
    35461 gtatttggat agaaatcgag atcaacatac agtgcaccag tgaccaggtt tccggttttc
    35521 agcgatccgc gtaaaccacg tttcaacaat tcgccaaggt gctcaacaac atccgcattt
    35581 tcgccaagct gcattttcag ccgctctggc tcgatacgaa tcagtaccgg aatacggtaa
    35641 tcatcgttaa atgtctgacg catattcggc gcaaagaatg gcactttgct tacggtaccc
    35701 aggcgaatac cccggaactc taccggagct cccggttgca gaccgcgtac cgaatcttta
    35761 aagaacatca gataatcaat gtgatcggtg tacaacgaat cctgaatgct cttctgatca
    35821 tcatacaaaa cgaaagctgt tttcggtgcc actggctgcc ctaaatccag accttccggc
    35881 acatcaaagc tgacaccgcc actcagcagc gttgtcaatg agcccatctc cacacgcatc
    35941 cctgctgacg tcagatcaac cgcaatgcca ctatctttcc agaagcgaac attgttggtc
    36001 accagtcggt cataaggtgc attgatgaac agttgatagc tgatattgcg tttttgtgta
    36061 tcgaaggtgc tggtttcaac cgaacctacc cgatagccac ggaacagcac cggatctcct
    36121 ggcgagagct gcccggcttt tttgctatcg agaatcacac ggataccttt cgcatcaggc
    36181 ggggccaacg gtggcgagtc cagcaaatcg tatttatcca ttttgctgcc tttcgcgcct
    36241 ggctgcagtt cgatataaac tccagacagc agcgttccca ggccgctaat cccttcgcga
    36301 ccaatctgcg gtttcaccac ccaaaagacg gtgtctttat gcagcaattt ttccataccg
    36361 gaattcagcc gcgctttgat ttcaacgtgc gtcaaatcat cagccagtgt ggcgctttca
    36421 accacgccga cgtcaacgct acggctttta atggtggttt tgccaccttc aattccttcc
    36481 gcattcgcgg tgatcagggt cacttccggt ccctgatggc tgtaatgata aaaaagaacc
    36541 caggccccaa tgagcgccgt gacgatagga aatatccaca cgggagacca gttcttcact
    36601 ttctggattt tggcttcccc attattagat tccatactgt caggactcct catgctctga
    36661 ttcaggttga cgatcccacg acaaacgcgg gtcaaacgtc atagcagaaa acattgtcat
    36721 tatgacgact aaagcaaaca ttaatgcacc cattgccgga taaatactca ttaaacctcc
    36781 catacgcacc agcgccgaga gcaccgcgat aacgaaaacg tcaatcatcg accagcggcc
    36841 tacaaactca acaacttcat aaatcaaatg cattctttca ctgtcgcgct tgccatgccc
    36901 tttggcatcc caacacagcc acgcgatggc gatcatcttt aacgttggca ccataatact
    36961 ggccagaaag atcaccgcag cgacgggata agagccttcg ctccataaca gaatgacccc
    37021 agcgagaatc gtcgacggca tcttcgagcc taataaatcc gtcaccatga tgggcaaaat
    37081 attagccgga agatacagca tgatggacgt tacaagcagc gcgagtgtcc actgcaggct
    37141 gtttctacgc cgaacgtacc ctttggtact acaacgcggg cacacgggtt catcagcagg
    37201 aaggattgcc gtacaacagg agcacgaacg cagcccctga cgtatccccg tgacgcctgg
    37261 ttttagcggc tggcgcagtt ctggcatcgg ggcgatgtcg tcccataacc agcgacgatc
    37321 aacgcactga aaagcgcgca gttgcaggac acaaaataaa caccagggga gaaagctgct
    37381 gcctacccca atgctgccgt aagccatcag tttaacgaaa ctgaccagca caccagcgag
    37441 gaaaatctcc gccattcccc aggttttgag ttgaaaaagc acccgtgcca gttgctcttt
    37501 taaacggacc ggtaattccg cgcgattcac cagtaacaga atggttatca gacaaaacgc
    37561 gggaaccagt tgcacaaaca acaggaaaaa ggtgccgagg ctggcgtagt cctcagaaaa
    37621 aagcacgccg ggaatttcca gtaatgtaat ttcactggta actcctgcaa cgttcatatt
    37681 cacaaaagga aacaagttgg acagcaacag catgaacagt gcagccaacg cataggcggt
    37741 gggacgctgc cgaggggcat cccacgccac ggttaacgtt gtgccacacc ggggacatgc
    37801 cgctttctga ccatgctcaa ggcgcggtaa cgccaccagc atgtcacact gcgagcacag
    37861 gatgtgcttc gcggcatgat gatgttcgca cattggtact ccttaatgat gcgctatttt
    37921 acagatgact aacaaagtgt tggttttcat gcctgataaa cccagttcat caggcaattt
    37981 taccgttgtc agctgtctct gtttaaatcg actattttgc gatcagccac cattttttaa
    38041 cgcttcaaga tactcccagc gttcaaaggc ttgctccagc tcctgctctg cagcagccat
    38101 atcagcaagc actttttgcg tctgctcatg cggctgactg aagaaggaag catccgccac
    38161 ttgcgtctgt agggcttcca gcttcgcctc cagatcttcg agcaattgcg gtagctgctc
    38221 cagttcgcgc tgcaatttat agcttagttt gctactgctg cgttttacag tttctgcttt
    38281 tgccgcggcg gcttcttcgg tttttttcac cgcaggctgt ttgagcgcca catactgctc
    38341 ttgctgacca cgggcatcat gataaccgcc gacataacga ccaattttac cgccgccttc
    38401 gaagatccaa cattctgtaa cggtgttatc gacaaactga cgatcgtggc taaccagcaa
    38461 taccgtgccc tgatagctgt cgatcagttc ttccagcagt tccagcgttt cgacatcaag
    38521 atcgttggtc ggttcgtcaa gaatcaataa gttgcttggt ttgaggaaca aacgcgccag
    38581 cagcaagcgg ttccgctcac cgccagaaag cgcacgtacc ggcgtcatcg cccgtttcgg
    38641 atggaacaga aagtcctgca aatagcccaa tacgtggcgt ggcttgccgt taaccatcac
    38701 ctcttgctta ccttcggcaa ggttatccat caccgtttta tcgggatcca gttccgcgcg
    38761 gtgctgatcg aaataagcca cttccagttt ggtgccaacg tgaatacgcc cgctgtccgc
    38821 ttgaagctga ccgagcatca gttttagcag cgtggttttg ccgcacccat tcggaccaat
    38881 cagggcaatt ttgtcgccac gtagaacctg ggcagaaaaa tctttcacca gttgcttacc
    38941 gttaacctgg tagcaaacgt cttccatttc gaaaacgatc ttaccggagc ggctggcctc
    39001 ttccacctgc atctttgcgg tacccatcac ttcgcgacgt tcaccacgtt cgcgccgcat
    39061 cgctttcagg gcgcgtacgc ggccttcatt acgggtacgg cgtgctttga tcccctggcg
    39121 gatccacacc tcttcctgcg ccagtttgcg atcgaactcg gcattttgta attcttccac
    39181 gcgcagggct tcttcttttt ccagcaggta ctggtcgtaa ttccctggat aggtcaccag
    39241 cttgccgcga tcgagatcaa caatgcgcgt cgccatattg cggataaacg aacggtcgtg
    39301 ggagatgaaa ataatcgtcc cgttgaaagt tttcaaaaac ccttccagcc agtcgatggt
    39361 ttcaatatcc aggtggtttg tcggttcatc aagcaacagc acgcgcggat tactcaccag
    39421 cgcgcgtcct aatgccgctt tacgcaacca gccgccggaa agcgacgaca gcgcaacatt
    39481 aggatctaac cccagttgcg ccagcacttc gttgatgcgg ttttccagct gccacaagtt
    39541 gtggtgatcc agctgttcct gaaccttcgc cagttcgttg agatttttct cgctcgggtc
    39601 gttcatcacc aggcgcgaaa tatcgtgata gcgtttcaga tattccgctt gttcttcaat
    39661 gccttcggca acgaaatcat aaacgctacc ctcaacgtta cgcggcggat cctgttgcag
    39721 acgagctaca atcaaatctt gctcgtaaat aatgcgaccg tcatccagcc cttgttcacg
    39781 gttgaggatt ttcattaacg tcgatttgcc cgcgccgttg cggcccacca gacaaacacg
    39841 ttcgttatct tcgatatgca gttctgcgtt atcgagaagc ggcgcgtcgc tgaacgacag
    39901 ccatgcgcca tgcatactga ttaatgacat tactattcct ttaggctgcg gtaatcagcc
    39961 agcagttgtg gatctggcgg ttacgggcga aatcctggga gagcgttttt tgcgtaattt
    40021 cttgtgcttt cagtcccagt tttgccaggc cgtcgagatc catacggaag ccacgtttat
    40081 tgttcgagaa catgatcgtc ccacctgcac gcagcagacg tttcaaatct ttcatcagcg
    40141 ccagatgatc gcgctgaaca tcaaacgcat cttccattcg ttttgagtta gagaaggttg
    40201 gcggatcgat aaagatcaga tcgaactgtt catttgcctc acgcagccac gccaggcaat
    40261 cggcctgaat caggcgatgc gcacgcccgg tcaggccatt caaacgcagg ttgcgttctg
    40321 cccactccag ataagtacgc gacatatcca cggtggtggt gctgcgtgca ccgcctaatc
    40381 ccgcatgcac ggtggcgctg ccggtataag agaacaggtt gaggaaatct ttgcctttgc
    40441 tcatctgacc gagcatacga cgggcgatac ggtgatcgag aaacagaccg gtatcgaggt
    40501 aatccgtcag gttcaccaac aagtgagcgt tatattcggt aacttcaaga aactcgccct
    40561 tctcgcccag tttctggtac tgatttttgc ccttctggcg ttcacgggtt ttcagcacca
    40621 gtttgtttgg cgcaatcccc agtaccgaaa tggttgcagc gataatatcg aacagacgct
    40681 gacgcgcttt gtgagcatca atagtttttg gcggcgcata ctcctgcacc accacccagt
    40741 cggcataacg gtcaacggca acgttatatt ccggtagatc ggcgtcatac aggcggtaac
    40801 attcaattcc ttcctggcga gcccacttct cgaatttttt gaggttctta cgcaggcggt
    40861 tggcgtagtc ttccgctacc atcgccggtt tgctgtctgg agtgctttcg gcaacgtggt
    40921 aatttttctg tacgcagtcc agcgggccgt ttttcgcctt gtactgtttg tctgcacgca
    40981 gctgcaagca gcttagcaga tccggcgagg cactaaacaa agagagattc cagccaccaa
    41041 actggttttt catgatccga cccagcaggc tatgcagcgc aatcagcgcc ggttcgctgt
    41101 ccagacgttc cccgtatggc gggttgctca acactgtgcc gtacggccct ttcggcagcg
    41161 gattggtcag ttgcgcgaca tctttcacct caaaggtgat cagttcacca atccccgcaa
    41221 gacgggcgtt agtgcgtgca cgttgaatca cccgtgcgtc gctgtccgaa ccgtaaaaat
    41281 gagagctata ctcagccagg cctttacggg cgcgagtttg cgcttccgct ttcacttcct
    41341 gccatatagc ttcatcatgc tgcgcccagc cgctaaagcc ccaacgccca cggtgcaagc
    41401 ctggtgcgcg atcggtcgcc agcatcgccg cttcaatcag caacgtagcg gaaccacaca
    41461 tcggatcgag cagcggtgtt cctggctgcc agccggatcg catcacaata gcggctgcca
    41521 gggtttcttt gatcggcgca ataccagcac gatcgcgata gccacgcaga tgtaaaccat
    41581 caccactgag atcaagggcg atactggcgg tttctttatg cagccagacg ttaacgcgga
    41641 tatccggcgc atcgcgatca acatttggac gcggcagatt tttccgcgtg aaagcatcga
    41701 cgatcgcgtc tttcactttc atcgcgccgt actgactgtt gcggatggtg tcattcaaac
    41761 cactgaagtg gacagcgaag gtcgcgccag gattaaacat ctctgtccag ttgatcgcct
    41821 gaacaccgag atagaggtct aaatcgctgt aaaccttaca ctcgcccagc ggcaacataa
    41881 tacgcgaggc caggcggctc cacatcaggc tctggtaaac aagccgtgtg tcgcccttga
    41941 aatggacccc accctgaacc acctggcatt caacggcccc caggttttcc agttcagttt
    42001 ttaacagctc ttccagccca cgggccgtac tggcaaacag agaattcata tcgtcacttt
    42061 tactctaaga gcctaccgca gtaggcgtaa ttgttgcagt cagtctggac atggacagcg
    42121 cggagaaacc ggagcgtaca caagtacgtg aggatttcga gcacaaccca gggccaaact
    42181 gacaaataaa atagcctaat gggacaggcg ctaagaaaat tgttgcgcat tatagctaat
    42241 aagcgcgtca tgtcataaag ttgagggctt attttcattt gaggaccgca ccgtggtgac
    42301 attaacccgg ctttttattc atcctgttaa atcgatgcgc ggcattggtc ttacacatgc
    42361 tctggctgat gtcagtggtc tggccttcga tcgcatcttt atgatcacgg aacctgacgg
    42421 tacgtttatt accgctcgcc agtttcccca gatggtacgg tttactcctt cacctgtgca
    42481 tgatggcttg catctcaccg caccagatgg cagtagcgca tatgttcgtt ttgctgattt
    42541 cgccacacaa gacgcaccaa ccgaagtttg gggcacacat tttaccgcgc gaattgcgcc
    42601 agacgcgatc aacaaatggc taagtggatt tttctcccgc gaagtgcaat tacgctgggt
    42661 ggggccacaa atgacccggc gcgtgaaacg ccacaacact gtacctctgt catttgctga
    42721 tggctatcct taccttcttg ctaacgaagc ctcgttacgt gatctccaac aacgttgtcc
    42781 ggccagtgta aaaatggagc aattccgccc caatctggtg gtttccggcg cgtcagcctg
    42841 ggaagaagat agctggaaag tgattcgcat tggtgatgtg gtgtttgatg tggttaaacc
    42901 ttgtagccgc tgtattttca ccaccgtcag cccagaaaaa gggcaaaaac atccggcagg
    42961 cgaaccatta aaaacattgc aatctttccg cactgcccag gataacggcg atgtcgattt
    43021 tggtcagaat ttaattgccc gtaatagcgg cgtgattcgc gttggcgatg aggtggaaat
    43081 tctggcaacg gctccggcaa aaatttacgg cgcagctgcc gctgatgata ccgccaacat
    43141 cacgcaacaa ccggacgcaa atgtagatat tgactggcag ggacaggcat ttcgtggaaa
    43201 taaccaacag gtgttgctgg agcaattaga aaatcaggga attcgtatcc cttattcttg
    43261 ccgcgcgggc atttgtggaa gttgccgtgt tcagctttta gaaggcgaag tcacgccgct
    43321 aaaaaaatca gcaatgggcg atgatggcac cattctttgc tgtagctgtg taccgaagac
    43381 tgcacttaag ttggcgcgtt agactgcctg ttcgaggctg aagctgtcaa cattaacctg
    43441 cggtttcagc ctgtcgttca tgattttaat ggcgtcgccc aactgcatcg cgcgacccgc
    43501 aataacaacg cagggttgtg ccagcaggca tagtgcggca ttttcgccag attcaacgac
    43561 cagcaaattg acctgctcgt gagtatcgct caaccaaacg caggccgcat cgcccggcat
    43621 gggcacccac atttcaccat gggaaacaaa atgccagctt ttcggcattt gcggtttgag
    43681 atagcgaatt gctaccagtg cattcagcac cagttcggct ttttgttctt tagataaatt
    43741 aaaatcgcgg cacttttctt caaaagagaa atagagcgcg gcgtcgtcaa cgcaaaagcc
    43801 tgcgggagaa aaggcatctg gagtcaacat cttgcgcgca aaacgtgagc gaaatagcat
    43861 accattggct aaatcgagca tcattcgatc gtgctcttca tcgtaatacc aacgccagtt
    43921 atcgtctggt ttaattcgca tctgcctctc ccctacccta taaaaccgca gcaacacaat
    43981 ccattgtcgc ttcgcttaat aacctcaaca ataaaagtac aaaatgtcta aataagcaac
    44041 agcgatgaat ataaaacaac cagggctgaa aataaagccc tgattatctt ataaagagga
    44101 attcgaagaa ataattagat atgggtaacg atttctttaa tcagcggcgg acctttaaaa
    44161 ataaaaccag aataaatttg caccagtgag gcacccgcag caatcttttc acgcgcagcg
    44221 ataaccgagt cgatgccgcc aacaccgatg atcggtaagc gaccgtttaa ttccagtgac
    44281 aagcggcgaa taatttcggt gctttttaac tgaagcggac gaccacttaa gccaccggtt
    44341 tgatcgcaat ttttcattcc ctgaacaaga gaacgatcga gtgtggtatt ggttgcaata
    44401 acgccatcaa tattatggcg aactaaacta tcggcaacct ggatcaattc ttcttcagaa
    44461 agatccggcg cgatcttcac tgcgatcggc acatatttat ggtgcatcgc ttgcaaatca
    44521 ttttgcttat ttttaatcgc ggttaagaga tcatccagcg cttcaccata ttgcagcgtg
    44581 cgtaatcctg gggtattcgg cgatgaaata ttgatggcga tatatcccgc ataggcatag
    44641 attttttcca tacaaatcag atagtcatct ttgccctgct ccactggcgt atctttattt
    44701 ttgccgatgt taatacccag gacgccgtca taatgggcct tttttacgtt ctctacgagg
    44761 ttatcaacgc caagattatt aaagcccata cggttgatca aaccttcggc atctaccaga
    44821 cgaaagagac gcggcttgtc attacctggc tgtggacgtg gcgtgacggt accgatctcg
    44881 atcgatccaa atcccatcgc gcctaacgcg tcaatgcact ccccgtcttt atcaagaccg
    44941 gctgccagac caagcggatt tttaaacgtc aggcccatgc agttaacagg tttcgcaggc
    45001 actttctgcc gcaccagtgc ttcaaacggc gttcctgtaa tacggcgtaa ttgctgaaaa
    45061 gtaaactcat gagcgcgctc tggatcgagc tggaaaaggg ctttacgaac gaaggggtag
    45121 tacatgaact ctcctggatt cccggtgtgc aaaccggggg cgtattatgt gcgagttcgg
    45181 accaaaaggg aattgacctg cggcaaaaaa aacgcaaacg ttttccgaac ctgtttcagg
    45241 gcagattttg ctccttcttt gcactcaacc cgccgtaatc atttatcgcc cgccaggcga
    45301 catgacacgg tgcgattgcc tgacaccagc ttgtctgacg ctggctttta ggcggcacaa
    45361 ctcccgcatt acttaacgct ttaccattta cgctcaactg aatcaggtta atgtagtaag
    45421 gcgttgggtt agtgagttgt acaccctgcg aattccgtgt ccagcgtaat tgttgataag
    45481 cttccagcgg gtcgcccggc aaaccttcgg gtcgccagaa cagtttaaat accgagcgca
    45541 tcgccacttt tacgctctga ttttcaactt tgccggatgg cacgctggca atgcttaact
    45601 caaatagcgt ttcgcgatcc acaggcaaga tgtcgctttc cgttctcagc aagcgcaacg
    45661 tgcctgtagt accgggcttc aggagaataa gtggcggagc ggccagtaat ggtgctggca
    45721 ctgtcgacgc ttcccccccg gcccaacgcg ttgggcggtt gattttactg ttaataagcc
    45781 aggattcctg actggtatta gtcagtaaaa tagatatcga ttctctgtct gccggaaaga
    45841 taaatcgcgt tccgccgacc accactcctg cccgcaattc acccgccacc agcagcgata
    45901 aaacggcgaa aatcaagagc gccaatctgt tccacgtatt agtcataatg aagtacaaat
    45961 gtcgcgctgg cattcacttt acccgactgc acatcgctat tgacaggtcg caattgcgca
    46021 taaaaagtta atggcacctt accgctatgt aggctgtagt ctttactcgc ctggttaagc
    46081 ggaatcaata tcttcgcatc atccagaatc gcaattgcca gcccgctggc tgcatccgta
    46141 ccactttcaa tggacagcat ttcgctctgc actccgtcgg gggttcctga aaaggtgacg
    46201 tcaaccgtgc tgacatctgt tccgcaatcc tgcaaattaa tgatgaaaga tttctccggg
    46261 ccataattcc catgaaaaaa acgtgtcagc gagacatcgc ctaactgcac attgatttgc
    46321 ttgctatcct gtgccagcac gcatgtatta cggcgaaacg atccgctaat gttgactgtg
    46381 ccatcatggg caaatagctc acagcccccc gttaatccca gtaagaacag aatccagatt
    46441 atccgtttca acatcactcc ttcactggta aaccagatca aaatagagaa ccgccgtcgc
    46501 attaccgccc gttgctcccg cctttgtgga cttataacga agttgatatt tgagtgtgtt
    46561 atccccggct tttaagggcg taagaggctg gacctgattg agcgggattt cttgctgact
    46621 ttgcgcatca agaatttgca cggcaatccc cgttgccata ccaccgcttc cgcctgtgtc
    46681 agttagtttg agcagtgtcg gcgcaagggt gtcagcctcg ccgctaaaaa ggacgtccgc
    46741 gccagtaata ccagcggcac aaccggtgat atcgatattc aaatcagcag cagtgcttac
    46801 ggtattcgcg gcctgaaaat cactgatatt aaaatcacca atatgaatat tttgtaatgc
    46861 acttttggtg gcgacatcac agccctgatc gaccacattc ccataaatat gcatactaac
    46921 ttgttcaggc ccagccatcc cgacatggat gttaagaatg ctaaagaaca agataacgca
    46981 ctgaaatatc gtttttttct tcatacgttc acgcgttaat caaaatttat tctcaccgtt
    47041 gccgtggctt caaatcgccc ggtttctggt ggtacacctg tcgtgctgac tggatagaca
    47101 ttcattcgcg cgatactgtc tgcatcatca ggaatagaga gcggtaatgt tccaccagca
    47161 ggggaaataa tgttgttctg gctatcggtc accaccacgc ccacgccagg acgtgacgtc
    47221 ttaatcgcct ggggatagct ggggtctgtg gttgccgtca gactcaggtt gacgttaact
    47281 aaccccgcgt ttcccgtgtt acacctgact ggaattgcga gttctgccgt tctggcccca
    47341 aggggtttat gccctacaac acgaaagtta gccgcaaata cgtcgcccag atcgacctcg
    47401 atagtctctc cggcattgac ggaacatgag ccaagtgaat tgatcgtacc agacaggtaa
    47461 tacactaaaa ccgatgtacc tgtagtcgtg caggaatcac ctgcgggtat gttgtagcat
    47521 tcaaataatc gcgccagcgc cataggagga atgacgaccg aaccaacgaa cggctgcaat
    47581 attttcaggg aaaagctacc gctattaccg ctagccccct ggccacctaa acgttgaccg
    47641 ccgggatcgc aaatatgcca tcgtgtatct gcccctgtgc ccacgttttc gaaaggaacc
    47701 gcagtaggag aactgccttt aacctgcaat aaaactttgg cggcaagata gtcattaatt
    47761 ttgtaccagt tgtcgcctaa atagactaac ggtgaatccg cggcgaatgc ccatcggcca
    47821 cttctgacat tgtctttatc gcaatcacag gaaacataat aatcattcga ttcgttccac
    47881 tgataaaatt tgtctttaac tactccagcc gtattttctt cttcctccag ggttttaacg
    47941 ccaaaattga cgccaatata cggattcccc tgagtggtat gacactgccc gctataagca
    48001 gcagtttgag cgcaccatag cattaagacg gcggcaaaaa atagtcgtag taataacacg
    48061 catttttctc caaagattat ctgcatatgg catccattcg cgtaatcgca gagttaattt
    48121 gttgttcggt aagctggtaa ctgatatgac aaatttgatc gggagcttcg ccccagcgta
    48181 cagttatcgc tccctgagcg ggcaaaccgg acatatagag cgagccatca tcatcaataa
    48241 tgccgctatt agtctgtatg ccgtccagcg tcgctatcgc gccaaaacgc agcggtatac
    48301 cctgctttga tgttttcatt aatacccttg ctcccgcatg ggcattgaac tccgccagaa
    48361 ccaacgcccc tttggtggga accacgttga ctactgcatt ttcaagatcc acgttacgtt
    48421 ttaacgatgc cgcatcaagt gcgattcgat tttcccgata agaagtggca taggggacaa
    48481 cggtatatcc gcgccagtca gttttcagcc cggtgctgtt cgccacacga gtattctctg
    48541 cgcctggtgc tgcaatcagg acgttagttt cccccagcga ttgccccagg gtaatgccct
    48601 gtgaatgggc aactaaactg cctgaaagag cgtagttcag ttgttgttga ctgccgttat
    48661 cgctgtagtt gtagcccact cgggcatctg caaacgcccc tttgtagtcc atgctggcgc
    48721 taccattagc cgttttcccc tcgctgttat atccctgttg cacgctgtag ctcaggttac
    48781 cgtcatcgag tagtgtctcc gttaaaccca ggttttgtgt aatgtgccct tcgttatcga
    48841 tactggtgtt agaggtcatc catgcgtaat tttttttcct ggtatagcta tcattacttc
    48901 tggggagcaa cagattgagc ggcagggaaa aatttagtgc aaacacctga tcagcatcag
    48961 gttgaccacg ggacttactg tagttccagg aaacccccag cgagagatct ttaatttgcg
    49021 tgttgtagcc aaactgtaat agccgatcct gttgatcggt atgccagtaa gtttgctggc
    49081 taccacttaa ataaaacgaa ccgtactcgc ctaattgctg ggagatattg acctgcagtt
    49141 tgccacgttt ggtgtaaaac aaattgtaat aacgtgacca catcggcgta tcttcatcat
    49201 caccgtcatt aaattcgtag ccatccatat gtttatacat ggtgtcggaa agggtgtaga
    49261 aacccgacgt cgaatagcga tagcccatca attgaaaatt agtgcccaat tggttgagcg
    49321 ttttggcata cagaaaacga tatgactgac ctttatgttc gctttggtct gccagcgtac
    49381 tcttcgcctg ggtcacatcg aacgaaattg ctccgaaatc gccaaggtta aatcccagac
    49441 caaacatggc ggcacggtaa tattcagcat attgtccacc accataccat gtcgttcccc
    49501 acggtccgcc ccactgcaac gtggcctggg caaatttgct ctcctgctgt tcattactat
    49561 tggttctgta tttcgccagt gtcaccgcgt atttgattcg cccctgacgc tggagtaatg
    49621 gcacgctgga aaacggtacg ctgtagctat tgacgctgcc gtccgcttct ttgatttcaa
    49681 ctaacaaatc accgctcgac gacgtggaat agagatcact aatttcaaaa gcaccaggcg
    49741 atacataagt ttggtaaatg gtgtaaccat tttgcttaat cgttatctgg gcattgcttt
    49801 tggcgatacc acgcaccaca ggcgcaaacc ctttcaggct gtctggcagc atgttgtcat
    49861 ctgacgccag ctgcacacca cgaaagctga cactatcgaa aaaatcgcct gcagtataat
    49921 catcacctac ggtcagttcg ccttttagtg gaataaccgc ccgctgcaaa tagctgctga
    49981 gattcttcca ttctgcggtt tggccactac tgcgactcca tgttgaattg ttgcgcaatc
    50041 tccatgggcc taaattaacg ccactgttta aattcagaaa atagctgtcg ccagaatcac
    50101 tgtctgcgct gctatgaata ctgttagccc cgctaaacga atatcccagt aatagcgcgt
    50161 tgatcccttc atcccaacgt tcagggggga cataaccacg ggcattactt ttgatggcga
    50221 tttgcggtac tgaaatttcc agacggagtt tattcacatt aaacgttacg ctggcatccg
    50281 gaataatctc cgccaatggc acacacgctt gtttgtccag ttgtttaaaa gccggaaacg
    50341 catcagtatt aacacccatt gcatccaggc tttcagtcgt caaacaggcg gcaatcccct
    50401 tctctggcgt tatctcaaca aaattgacgt tgcgcgtatc tacaattgtc tgattcagaa
    50461 caatatcgac acgataaacc cctggcgtta ttttttgtcc tttttcgaaa cgagacaaat
    50521 cggccaccat tgagggatca tctttgaaaa aagcaggatc gaaccaggtt tcagcttgtg
    50581 cattgaacgc tgtcgacaca aacaccacca gcccaccaat aaacgatggc actccaccag
    50641 agcttaacag gctgtgttgt cgatgagttc tatacatctc gctctcttaa ctgaagacgg
    50701 cttacgattt acagttgagt tgcggcgttt ccgccccgta gtcattgatg gttcgatacg
    50761 ttacgctgct atttttggcc ccctttggca gagaaaattg gtatttatca aagggggaaa
    50821 tcatttgatt tttaactact tttccaccta ttttcaggtc tgtcagggta atgaaataag
    50881 gtgttgggtt actgaccgta accatgtcag ctgacgaagt gcaacgcagt ttcgccggag
    50941 cctcgcctaa gggatacgct aaaccttttg gtcgccagaa aagtttgatg acgctttgtg
    51001 tggcaatctt cagggtattt ccctcttccc gcttcgttgt tgaagggact gcccgtacgc
    51061 tagtgaagaa aagggtttct ctgtctttcg ccaacggcgc accaatgtac ataatgcgta
    51121 acagattctc attagccgga ttcagcacaa atagcggtgg tgtaataata aaatctgctg
    51181 attttttatt atctggggtc atcacccatg actgaataag ataaggaaca tccggactgg
    51241 tgttacgtac acccagtgaa atttgctctt caccttctga ataaacaaga cgtgttgctg
    51301 caagtccaat cccgccttta cctgccgccc atgcggatga gcagctgagt aatattgccg
    51361 ttaaacttac ggtcacaatt ccctttgtta tgcaagtttt cataaatagt cactcttaaa
    51421 aaatatgaat
//
LOCUS       DAPGWD010000015        50414 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPGWD010000015 DAPGWD010000000
VERSION     DAPGWD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.015"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1726)
                     /gene="rsxC"
                     /locus_tag="RHX88_000837"
     CDS             complement(<1..1726)
                     /gene="rsxC"
                     /locus_tag="RHX88_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDV2229564.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGHGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQ"
     gene            complement(1719..2297)
                     /gene="rsxB"
                     /locus_tag="RHX88_000838"
     CDS             complement(1719..2297)
                     /gene="rsxB"
                     /locus_tag="RHX88_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDV2229565.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2297..2878)
                     /gene="rsxA"
                     /locus_tag="RHX88_000839"
     CDS             complement(2297..2878)
                     /gene="rsxA"
                     /locus_tag="RHX88_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDV2229566.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(2955..3395)
                     /locus_tag="RHX88_000840"
     CDS             complement(2955..3395)
                     /locus_tag="RHX88_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDV2229567.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(3481..3696)
                     /gene="ydgT"
                     /locus_tag="RHX88_000841"
     CDS             complement(3481..3696)
                     /gene="ydgT"
                     /locus_tag="RHX88_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDV2229568.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(3969..4094)
                     /gene="blr"
                     /locus_tag="RHX88_000842"
     CDS             complement(3969..4094)
                     /gene="blr"
                     /locus_tag="RHX88_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDV2229569.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            4337..5377
                     /locus_tag="RHX88_000843"
     CDS             4337..5377
                     /locus_tag="RHX88_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV2229570.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(5412..6413)
                     /gene="add"
                     /locus_tag="RHX88_000844"
     CDS             complement(5412..6413)
                     /gene="add"
                     /locus_tag="RHX88_000844"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDV2229571.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(6517..7689)
                     /gene="malY"
                     /locus_tag="RHX88_000845"
     CDS             complement(6517..7689)
                     /gene="malY"
                     /locus_tag="RHX88_000845"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDV2229572.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(7699..9291)
                     /gene="malX"
                     /locus_tag="RHX88_000846"
     CDS             complement(7699..9291)
                     /gene="malX"
                     /locus_tag="RHX88_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDV2229573.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            9466..10494
                     /gene="malI"
                     /locus_tag="RHX88_000847"
     CDS             9466..10494
                     /gene="malI"
                     /locus_tag="RHX88_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDV2229574.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            10606..11373
                     /gene="hdhA"
                     /locus_tag="RHX88_000848"
     CDS             10606..11373
                     /gene="hdhA"
                     /locus_tag="RHX88_000848"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDV2229575.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            11602..12192
                     /gene="uidR"
                     /locus_tag="RHX88_000849"
     CDS             11602..12192
                     /gene="uidR"
                     /locus_tag="RHX88_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDV2229576.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            12581..14392
                     /gene="uidA"
                     /locus_tag="RHX88_000850"
     CDS             12581..14392
                     /gene="uidA"
                     /locus_tag="RHX88_000850"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDV2229577.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            14389..15762
                     /gene="uidB"
                     /locus_tag="RHX88_000851"
     CDS             14389..15762
                     /gene="uidB"
                     /locus_tag="RHX88_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDV2229578.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            15801..17066
                     /gene="uidC"
                     /locus_tag="RHX88_000852"
     CDS             15801..17066
                     /gene="uidC"
                     /locus_tag="RHX88_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDV2229579.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGMGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(17111..18619)
                     /locus_tag="RHX88_000853"
     CDS             complement(17111..18619)
                     /locus_tag="RHX88_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV2229580.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(18720..19895)
                     /gene="manA"
                     /locus_tag="RHX88_000854"
     CDS             complement(18720..19895)
                     /gene="manA"
                     /locus_tag="RHX88_000854"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDV2229581.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20094..21740
                     /gene="fumB"
                     /locus_tag="RHX88_000855"
     CDS             20094..21740
                     /gene="fumB"
                     /locus_tag="RHX88_000855"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV2229582.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIMVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            21883..23286
                     /gene="fumC"
                     /locus_tag="RHX88_000856"
     CDS             21883..23286
                     /gene="fumC"
                     /locus_tag="RHX88_000856"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDV2229583.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(23283..24212)
                     /gene="tus"
                     /locus_tag="RHX88_000857"
     CDS             complement(23283..24212)
                     /gene="tus"
                     /locus_tag="RHX88_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDV2229584.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGARVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(24288..25589)
                     /gene="rstB"
                     /locus_tag="RHX88_000858"
     CDS             complement(24288..25589)
                     /gene="rstB"
                     /locus_tag="RHX88_000858"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDV2229585.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(25593..26312)
                     /gene="rstA"
                     /locus_tag="RHX88_000859"
     CDS             complement(25593..26312)
                     /gene="rstA"
                     /locus_tag="RHX88_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDV2229586.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            26441..26776
                     /locus_tag="RHX88_000860"
     CDS             26441..26776
                     /locus_tag="RHX88_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDV2229587.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(26773..27495)
                     /gene="folM"
                     /locus_tag="RHX88_000861"
     CDS             complement(26773..27495)
                     /gene="folM"
                     /locus_tag="RHX88_000861"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDV2229588.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(27532..28914)
                     /locus_tag="RHX88_000862"
     CDS             complement(27532..28914)
                     /locus_tag="RHX88_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2229589.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAYAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(29100..30044)
                     /locus_tag="RHX88_000863"
     CDS             complement(29100..30044)
                     /locus_tag="RHX88_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2229590.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            30568..32100
                     /gene="pntA"
                     /locus_tag="RHX88_000864"
     CDS             30568..32100
                     /gene="pntA"
                     /locus_tag="RHX88_000864"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDV2229591.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCTCSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            32111..33499
                     /gene="pntB"
                     /locus_tag="RHX88_000865"
     CDS             32111..33499
                     /gene="pntB"
                     /locus_tag="RHX88_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDV2229592.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGNDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(33524..34558)
                     /gene="tqsA"
                     /locus_tag="RHX88_000866"
     CDS             complement(33524..34558)
                     /gene="tqsA"
                     /locus_tag="RHX88_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDV2229593.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDCVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            34970..35335
                     /gene="mdtJ"
                     /locus_tag="RHX88_000867"
     CDS             34970..35335
                     /gene="mdtJ"
                     /locus_tag="RHX88_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDV2229594.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            35322..35651
                     /gene="mdtI"
                     /locus_tag="RHX88_000868"
     CDS             35322..35651
                     /gene="mdtI"
                     /locus_tag="RHX88_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDV2229595.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(35690..36511)
                     /locus_tag="RHX88_000869"
     CDS             complement(35690..36511)
                     /locus_tag="RHX88_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDV2229596.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(36611..36694)
                     /locus_tag="RHX88_000870"
     CDS             complement(36611..36694)
                     /locus_tag="RHX88_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229597.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(36787..37122)
                     /locus_tag="RHX88_000871"
     CDS             complement(36787..37122)
                     /locus_tag="RHX88_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDV2229598.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            complement(37519..38772)
                     /locus_tag="RHX88_000872"
     CDS             complement(37519..38772)
                     /locus_tag="RHX88_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2229599.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            38879..39772
                     /locus_tag="RHX88_000873"
     CDS             38879..39772
                     /locus_tag="RHX88_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2229600.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            39907..41127
                     /gene="mlc"
                     /locus_tag="RHX88_000874"
                     /gene_synonym="dgsA"
     CDS             39907..41127
                     /gene="mlc"
                     /locus_tag="RHX88_000874"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDV2229601.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            41252..41947
                     /gene="bioD"
                     /locus_tag="RHX88_000875"
     CDS             41252..41947
                     /gene="bioD"
                     /locus_tag="RHX88_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV2229602.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(41900..43192)
                     /gene="clcB"
                     /locus_tag="RHX88_000876"
     CDS             complement(41900..43192)
                     /gene="clcB"
                     /locus_tag="RHX88_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDV2229603.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(43352..43966)
                     /gene="dmsD"
                     /locus_tag="RHX88_000877"
     CDS             complement(43352..43966)
                     /gene="dmsD"
                     /locus_tag="RHX88_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDV2229604.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(44009..44863)
                     /locus_tag="RHX88_000878"
     CDS             complement(44009..44863)
                     /locus_tag="RHX88_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDV2229605.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(44865..45482)
                     /gene="dmsB"
                     /locus_tag="RHX88_000879"
     CDS             complement(44865..45482)
                     /gene="dmsB"
                     /locus_tag="RHX88_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV2229606.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(45493..47916)
                     /locus_tag="RHX88_000880"
     CDS             complement(45493..47916)
                     /locus_tag="RHX88_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV2229607.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(47977..50403)
                     /locus_tag="RHX88_000881"
     CDS             complement(47977..50403)
                     /locus_tag="RHX88_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV2229608.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVIIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
BASE COUNT        12642 a        12480 c        13038 g        12254 t
ORIGIN      
        1 cctgttgctg ttccagcttg cgcgctttgg cgcgggcgat agctgcttca acggcagttt
       61 tacgtggatc agcaacggtt gctgcgtcgt tagtttgctg cagttctgcc tgtttcgctc
      121 tggcttgcgc tttacgggct tcccgtgctg caataattgc actgttatcc gggcgttcgc
      181 ccgctttaat cacaataggc tgtgtagcct gggcctgttt ctctttcacc cgcgccagag
      241 cggcagcaat cgcatcttta tctttggctg caggttgaac ggctgcgctc ttatgtcgtt
      301 caaggcgagc cgctttttcg cgctccagac gagcctggcg cgcttcgaaa cgcgctttgg
      361 cttctgcggc gcgcttttct tcctgacgaa tagccgcaat ttcagctttt tcctgacgga
      421 aatattgcac caggggaata ttgctcgggc aaacccacgc gcaagcccca cattcaatgc
      481 aatcagcaat gttatgcgtg gtagctttat cgtgttgctg acctttgctg aaccagtaca
      541 actgttgcgg caaaagatcc gcagggcagg cgtcagcaca ggcgctacac cggatgcagc
      601 tttgttcttc ctgtggttcg ccaagttcat tggcagaggg agccaacaga cagttggtaa
      661 tctttacgac cgggacatcc agccatggca aggtaaagcc cattagcggg ccacccataa
      721 tcaccatttg atcggcagag gggcagaatc cggcatcatt caataaatga cgcactggcg
      781 tccccagccg tgcccagacg ttgcccgggc gagcgattgc ttcgccagtc agggttacaa
      841 cacgctcggt aatcggctcg ccatcaataa cggcacgttt cactgcataa gcagtgccga
      901 cgttttgcat taatacgccg atatcggatg aacgcccgcc atgtggaacc tgcttcccgg
      961 tcagaatgta ggttaattgt ttagcaccgc cagaaggata tttggttgga atcacccgca
     1021 gagaaatatc gttagagtcc gccagcaccg cgcgcagcat ggaaatcgcc tgcggtttgt
     1081 tatcttcaat gccgataaga atttcgcgtg gctgcagaat atgcgcaaga atgcgaatac
     1141 cctctacgac ctgagccgcg caatcctgca tcaaacggtc atcggcggta atgtacggct
     1201 cgcactcagc cgcgttgata atcaacgttt caatcttatc tccgccaccc tgcaatttaa
     1261 cgcctgtcgg gaatcctgca ccgcccagcc cggctacacc aaactgatgt atgcgctcga
     1321 ttaactcttc gcgactgcga gtgcgataat cggcccagcc gtcgcgcggg atccagcagt
     1381 cttcaccatc ggcatcaata atcacgctta attcagctaa agctgaagga tgagccgtag
     1441 agtggggcgc aatagccgta acggtacccg aggtgggcgc gtgaacaggc agcattttgc
     1501 cgtgaccacg ggtaagcggc tggccgcgca atactttatc gccgacgcta acgcacaact
     1561 caccttcagc gccaatatgc tgtttcagtg gaataacaaa acgctgcgcc agtggtacct
     1621 ggcgcagggg tgtaccgttg gactgggttt tcatctccgg tggatggatg ccgccgttga
     1681 aatcccagat tttatttttt ctgaatgcag agaataactt aagcatggtg ttccacggga
     1741 atgatacgca cgggaatggt gttcagatcc catttccagg agtcaggtgt ttctgcgacc
     1801 ggttgcaacg agatgcagtg cgtcgggcac ggatcaacac ataaattgca gcccgtacag
     1861 agatcactca ttaccgtatg catggcacgg gtagcgccaa cgatggcgtc taccggacaa
     1921 gcctgaatac atttagtgca gccaatacag ttattttcat caataaccgc caccatccgc
     1981 gcaggcgtta gctcttgcgc ttcgccatcc agcggctgcg gctcgacatt aagcaactcg
     2041 gcaattttta gcatcacagc ttcgccacct ggggcgcaac ggttgatttt ttcaccgtta
     2101 cagctgatgg cttccgcgta ggggcgacag ccgggataac cgcactgacc acactggctc
     2161 tgcggtaaga tttcgtcaat tttctcaacg accggatcgt cttccaccgc aaaacggcgg
     2221 gaggcataac ccagaatggc gccaaacgcc aggcccagca ggctcacggc ggcaacggca
     2281 atccagatag cattcattac aacttcacca aaccactaaa gcccataaag gccagagaca
     2341 taagacctgc ggtaattaac gcaatggcat taccgcgaaa aggtgccggg acatcagcca
     2401 cagcaaggcg ttcgcggatg gcggcgaaga gcaccatcac cagcgagaaa ccgacagcgg
     2461 cggaaaaacc gtacagcgcc gactgcaaga aattgtgccc gagattgata ttcagcaacg
     2521 ccacgccgag cactgcacag ttggtggtga taagcggcaa aaaaatcccc agcagacggt
     2581 aaagcaccgg gctggttttg cgcaccacca tctcggtgaa ctgcacgacc acagcaatca
     2641 ccagaataaa tgccagggtg cgcaggtaaa tcagattaag tgggatcaaa atccacgtat
     2701 cgataagcca ggcgcaaata gacgccagcg tcatcacaaa cgttgttgcc agccccatgc
     2761 ccatcgcggt ttccagtttt ttggaaaccc ccataaacgg acagagaccg agaaacttga
     2821 ccagtacaaa gttattgacc agtacagttc cgacaaacag taacaggtag tcagtcattt
     2881 ttcggcctga aataaaaaag ccgcctatta tccgttaatc cagagcaggc gacaacaggt
     2941 taactgtaag gttattacgg attcacaaac gtcgctttta cccgcgacga gcgtttgaaa
     3001 taggggacga tcaatgcggt tgccaacaaa gtaaacagca actggcgaac ggctatgcca
     3061 tcttccacgg gtgaaaaagc gaatgctttt actgccagca atacggaaat aagcagccag
     3121 ataatatagt gtttaggaac acaacgccga cgtttaaaga aagcgatggt caaccacagt
     3181 gtgtaatacc acatggcgat agcggtaata aaagaaacgc cccataagat ttgtgtcgtc
     3241 agtgcttgtc cgccgagcgt ttgaaatgtt tgcggagaag ataacgcagc ggtatacagc
     3301 aacaacgcca gcgtcgtact caacagagcg actaaaagcc aggccaacgg gccaagcaac
     3361 cagcctccaa tacgttgtgg cgttgtggtg gtcataggtt ctccagaata gcgacgcaaa
     3421 caggcaaatc taattttagg ggcgttatta taaggctttt ataaccgatc gctactttct
     3481 ttattggaca tagtgccaga cggacttcgg tacctggccg aggtcaaaca aacgcccgcc
     3541 agaaaccagc tctgcgcgac ggtgatcggc agcacgatac atattgatca gttcctgatc
     3601 gtccgtcagg gtgtaattaa gatggtcgta gagtttttcc agactttcga gtgaactgat
     3661 tttgcgaaat tttaataagt agtcctgaac agtcataaaa ggtccataaa ataattaata
     3721 ccaaaggggt aggtattatt agtgatgata aaatttcacc aaaagtgaac gcttgtgtta
     3781 ttacaataat aagaatgcgc tgatggcaaa tattcaggga tgaaaaactg ctctgaaaag
     3841 agtaacgcgc tttactatcc ctgacaatca ccaacaacat cgaacaagat aataaattcc
     3901 tggtttaata tccgacaagt gaaaacatgc acccggacgg gcagcatgtc gctccacaag
     3961 tgcagagctt acttgtgttg taccgaagca ctctgttcag gtggctgata gttgtcaatg
     4021 tgactcgcca cgccaagaag aatgactgaa acgacaagaa cgatccaacc tgttaattca
     4081 ataagacgat tcattacagc ccacatctct cttgattgat ccattaactt cagggggtaa
     4141 atgttactta gcaataatag ctccagcaag atttttactg aggtttttgc gatatttagc
     4201 ttttgtcgtt ggaaaattcg ctattttttt gacttaagtt aaacaacatc ccttattgct
     4261 ggcaggttat taaactgttg agcgtgggta aaggatagtg tcaaatagcc atcatacttc
     4321 aatgagaggc aatgacatga gcgacaacat ccgtgttggg ttgattgggt atggttatgc
     4381 gagcaaaacc ttccatgcgc ccctgattgc gggcacgccc gggctggaac tggcggtaat
     4441 ctccagcagt gatgaaacaa aagtaaaagc cgactggcca acggttacgg ttgtctctga
     4501 gccgaagcat ctgtttaacg atcccaacat agacctgatt gtcattccta cacccaacga
     4561 tacccatttc ccgttagcca aagcggcgct tgaggcgggt aaacatgtgg tcgttgataa
     4621 accctttacc gtgacactgt cacaagcgcg agagctggat gcgctggcaa aaagcctggg
     4681 gcgtgtgctg tctgtattcc ataaccgtcg ctgggatagc gatttcttga cgctaaaagg
     4741 tttactcgcg gaaggcgtgc tgggtgaagt tgcttacttt gagtctcatt ttgaccgctt
     4801 ccgtccgcag gtgcgcgatc gttggcgtga acagggcggc ccaggcagcg gtatctggta
     4861 cgatttagca ccacatcttc ttgatcaggc cattacgctt tttggtttac cggtcagcat
     4921 gacggtagat ttggcacagt tacggcccgg agcgcagtcg accgattatt tccacgccat
     4981 cttgtcctat ccacagcggc gagtcatttt acacggtacc atgctggcag ccgctgagtc
     5041 agcacggtat atcgtgcatg gatcccgagg cagttatgtg aaatatggcc tcgatccaca
     5101 ggaagaacgt ctgaaaaatg gcgagcgtct accgcaggaa gactggggct acgatatgcg
     5161 tgatggcgta cttacccgcg tggaaggtga ggaacgtgtc gaagaaacgc tgttgacggt
     5221 gcctgggaat tatccggctt actatgcggc tattcgtgat gcgttaaatg gcgatggtga
     5281 aaatccggtt ccggcaagcc aggcaatcca ggtaatggag ttgattgagc tgggcatcga
     5341 atccgccaaa catcgcgcga ctttgtgcct tgcatgaaaa atggatggtg caatttgcac
     5401 catccatttt gttacttcgc ggcgactttt tctcgcagtg cgcgtttttc ctcagcgctg
     5461 aggaaagcca tttccagacc attaatctgt gcctggcgga tttgctcgcg ggataaccca
     5521 gcagctggcg cggcaacggt atattcgtga atgatatcca ctccctgtac gccgggatcg
     5581 tcagtgttaa tgctggcacg aatgccatgc tcaaggaacg ttttcagcgg atgtgcagcc
     5641 agctctgcta cggtgctggt ctgaatattg gaggtcagac aggattcaat accaatttgt
     5701 tgctcggcga gaaaatccat cagcgcccga tcttcaatgg cttttacgcc atgtccaata
     5761 cgctccgcac ccagttcacg aatcgcctgc cagatgcttt ccggcccggc agcttcgcct
     5821 gcatggacgg taatatgcca gcccgcatca cgcgcgcggt tgaagtgaga aaggaacaga
     5881 cttcccggga aaccaagttc atcaccggct aaatcaagtg cggtaatctg gtcacggtgg
     5941 gctaaaaagg cctccagctc ttgctgacag gcggcttcgc cgaaggtccg gctcataatg
     6001 ccgataagct tcgcctgcac accaaaggtg cggcaacctt cacgtacgcc atcgatcacc
     6061 gcttcgacaa cacccgctac aggcagctga tgtgccattg ccatgtagcc tggtgaaaaa
     6121 cgcagctcga catagtgcag gccgtgacgg gctgcatctt caatgttttc aaatgccacg
     6181 cggcgacagg catcaagaga ggcgagaact ttaacgcccc agtcaagttt ggtcagaaag
     6241 ctcaccagat cgggttcgtt ggcaatgacc tgaacgtggg gaatcagtgt ttccagggat
     6301 tgtgcaggaa gcgagatatt atactggcgg ccaagttcaa gaatggtctg gggacgaatg
     6361 ttgccatcaa ggtggcgatg gatatcagtt aatggcaggg tggtatcaat catggtcgca
     6421 ctctttttct gaatataaaa gtgcgcacca ttataaaaac aaagcagggt aaaaatccac
     6481 gattgcgcaa cgatttttta cgttgcgcaa ttggggttag cgaacagcgc ggatggcgtt
     6541 tattaatcca gccacacctt tttccagttt cgaacgtggg cagccggcat tgagacggac
     6601 aaaaccacga ccttcttcac cataggtata ccccggcatg atcgcgactt tttcttgctc
     6661 gataagcgct ttttgcaacg cgttgtcgtc aatattcaac ggacgtagat caagccaggc
     6721 cagataagtg gattgcggga tctgccagtt gagttcagga aacgcggcgt tcattttatc
     6781 tgagatatac gtcaggttat ctttcagata gactcgtaag gcatccagcc acggtgcacc
     6841 ttgctgatag gcggcgatat gggcagttaa cgccagtacc gaaggggaag aaagcccatc
     6901 acgtcctttc agtgccgata aataggcatc acggctgctg ctattttcta taatcccgta
     6961 agcaccggtc agcgcgggaa tattgaaact tttcgagccc gacgtcagca acgcccagtc
     7021 tccccgcgcc acattactcc agggaatatg cggctgctcg ccccagacca tatccatatg
     7081 gatttcatcg gaaataaccc gcacaccatg acgctcgcac aggtcagcca tgatctccag
     7141 ctcatcgcac gtccacactt tcccggtagg attctgtggg ctacacagga gcataatttt
     7201 acattctggt ttcgccaaca cggcttccag cttgcccata tcgcaaaacc agtcgtcagc
     7261 ctgcttctct aaagcaacgg gcattactgt gcgctggtta ccttcaatgg ccttgtaaaa
     7321 tgcgtcatag gcgggcgtgt ggatcaccac gccttcacct gtttcagacc actgacgaat
     7381 cagttctgaa accatataga tgacagaagg gccatacacc accgtctgcg tatcaattgc
     7441 ggtgtaatgc tgggtggaaa accagtgggc aatagccgcg agaaactcat cgtttttcca
     7501 gcggctgtag ccaaatacgc cgtgcatcag gcgctgattc agcgcctcga taatgcaggg
     7561 ggcagtggca aaatccatgt ctgaaatcgt gaacggtaac aggtcagcag tgccgaaacg
     7621 gtcagcgaca taatcccact gtgtacacca tgtgccatga cgatccacga cctttgaaaa
     7681 atcgaacata tcttatcctt atgcctggac agtatgcatc agaccggcca tttcatcttt
     7741 tactgactga acttgtggcc cgataacaac ctgcaggtta tgttgattaa gttgtactac
     7801 gccaattgcc cgattgtcct tcagtgcctg cacattaaca agcgacatat ctttcacaga
     7861 caaacgcaga cgggtaatgc agttatcgag gctgacaata ttgtcggcac cgcctaatgc
     7921 ttcgaggatt gcaggaacgt tgtaacctga tttacccggc gcaccggcaa cggctttttc
     7981 gattgagctg gcaacttcgc tatcgcgccc cggggttttc agattgaagc gggtgatagc
     8041 gaaacggaag atgacgtagt aaacgacaaa ccagattgcc gccaccactg gcaccatgta
     8101 ccacttggtt gacagaccat gcaaaatacc gaacaccacg aagtcgatga tattgccgtc
     8161 ggtattaccg atggtgacgc cgagcacaga catgacagtg aagccgaggc cggttaacag
     8221 cgcgtggatg acatacagaa ctggcgccac gaacaggaac aggaattcca gcggttcggt
     8281 cgtgccgcca acgacgcagg cgatcaggcc agaaatcagc agacctttaa ttttatggcg
     8341 attttccggg cgcgcgcagt gatacatagc taacgctgca cccggcagac cgccgagaaa
     8401 cgcaggcatt ttaccttgcg aaaggaaacg cgtggcgctt tcagaaaaac cgtgagtggt
     8461 cgggcaactc aattgcgcct ggaagatggt cagtgcgccg ctgacggttt gaccgcagac
     8521 ttcctgcgtg ccgcctgcgt cggtaaagcg aattaatgct accagaatgt gatgcagacc
     8581 aaacggcaac agcagacgtt caccggtacc aaacagcatc ggtccgaaat cacccgcgct
     8641 gtttatcata tggcccaagc cgctaatacc catggcgaaa atcggccaga ctaatggaat
     8701 caccaggccg acaaggccca tcaccagcga ggagataatt ggtacgaagc gcgtaccgcc
     8761 gaagaatgcc agcgcatccg gcaggcggat attatggaaa cgctcatgca gcatccagac
     8821 gataataccg gcgatcaccg caccgaggat cccggtatcg atcgactgga tcccaagaat
     8881 gctctggatg ttattggctt tcagaaccgc ggcatccgtg gttggcagaa tgcctttatt
     8941 ggtcaaccag aagtttaccg cgaggttcat taccgcataa ccgacgaagc cagcgaatgc
     9001 cgctacgcct ttattttcgc gtgccaggcc cagcgggatg gcgatacaga acatgacagg
     9061 caggaaacta aaagcaaacg agccaatctt actcatccag gtaaagatag cctgcaacac
     9121 ggggttgccc aggaccggga tcagggttat gacatcatgg ctgctaagag aactaccaat
     9181 gccgagcata atgccgcaga acgacaataa tgccacgggt aacatgaagg ttttgcctaa
     9241 ctgctggaag aactcccaca gcgtgacttt cggtgctgtt ttcgccgtca taaaacgact
     9301 cctcgtagag aacaatctgg attcagtaaa ttgcgcgaga agataaaacg ttttatcaaa
     9361 ttttagtgag gcataaatca cattacgcaa cgataatagc gggtataaga taaataaaag
     9421 gtaaaacgtt ttatctgtca cataatcagg gagtaggtca tctgcatggc taccgccaaa
     9481 aaaataacca ttcatgatgt tgcgctggct gcgggcgtgt cggtaagtac cgtttcgctg
     9541 gtgcttagtg gcaaagggcg aatctctacc gccacaggag aacgcgttaa cgccgccatt
     9601 gaagagctgg gatttgtgcg caatcgccag gcgtcggcgc tgcgcggcgg gcaaagcggc
     9661 gtcattggtt tgatcgtccg tgatttatct gcgccgtttt acgccgaatt gacggccgga
     9721 ttgacggaag ctctggaagc gcagggacgg atggtttttt tgcttcacgg cggtaaagac
     9781 ggcgagcagc tggcacagcg gttttcactg ttactgaatc agggtgtgga tggtgtggta
     9841 attgccgggg ctgcaggaag cagcgatgac ctgcgacgga tggcagaaga aaaagctatc
     9901 ccggtgattt tcgcttcccg tgccagttat cttgatgatg ttgatacggt tcgcccggac
     9961 aacatgcagg ctgcacagtt gttgacggag catctcattc gcaatgggca tcagcggatc
    10021 gcctggctgg gagggcaaag ttcctcatta acccgtgcag aacgggtggg gggctattgt
    10081 gcaactctac taaaatttgg cctgccgttt catagcgatt gggtgttgga gtgcacttcc
    10141 agccagaagc aagccgcgga agctatcacg gcgcttttac gtcataaccc gaccatcagt
    10201 gccgtggttt gctataacga aactattgcg atgggggcat ggtttggttt gctcaaagca
    10261 gggcggcaaa gcggggaaag cggagtcgat cgttactttg agcaacaggt ttcgctggcg
    10321 gcatttaccg atgcgacacc aaccacactt gatgatatac ctgttacctg ggccagcacg
    10381 ccagcgcggg aacttggtat cacacttgcg gatcgcatga tgcaaaaaat cacccatgaa
    10441 gagacgcatt cacgcaatct tattattccc gcccggctca ttgcggcgaa ataatcctct
    10501 ctttatctgc tatacctggt agtgtccttt cctcaaggtt aatgataaaa aatgtggcac
    10561 acaggcggtg ccttttattt ttactgaagt tcacaggagg tttatgtgtt taattctgac
    10621 aacctgagac tcgacggaaa atgcgccatc atcacaggtg cgggtgcagg tattggtaaa
    10681 gaaatcgcca ttacattcgc gacagctggc gcatctgtgg tggtcagtga tattaacgcc
    10741 gacgcagcta accatgttgt agacgaaatt caacaactgg gtggtcaggc atttgcctgc
    10801 cgttgtgata ttacttccga acaggaactc tctgcactgg cagactttgc tatcagtaag
    10861 ctgggtaaag ttgatatcct ggttaacaac gccggtggcg gtggtcctaa accgtttgat
    10921 atgccaatgg cagattttcg ccgtgcttat gaactgaatg tgttttcttt tttccatctg
    10981 tcacaacttg ttgcgccaga aatggaaaaa aatggcggtg gcgttattct gaccatcact
    11041 tctatggcgg cagaaaataa aaatataaac atgacttcct atgcatcatc taaagctgcg
    11101 gccagtcatc tggtcagaaa tatggcattt gacctgggtg aaaaaaatat tcgggtgaat
    11161 ggcattgcgc cgggggcaat attaaccgat gccctgaaat ccgttattac accagaaatt
    11221 gaacaaaaaa tgttacagca cacgccgatc agacgtctgg gccaaccgca agatattgct
    11281 aacgcggcgc tgttcctttg ctcgcctgca gccagctggg taagcggaca aattctcacc
    11341 gtctccggtg gtggggtaca ggagctcaat taatacacta acgaaccggt aaacagccgt
    11401 atgtgttgtt taccgggata gcgtgtgcaa ttcatcaacg tctctgctaa acaactggca
    11461 gccaaatcac ggctattggt taaccaatag ctggatgaaa agcatacgaa tagagtgtgc
    11521 cttcgcacta ttcaacagca atgataggcg ctcacctgac aacgcggtaa actagttatt
    11581 cacgctaact ataatggttt aatgatggat aacatgcaga ctgaagcaca accgacacgg
    11641 acccggatcc tcaatgctgc cagagagatt ttttcagaaa atggatttca cagtgcctcg
    11701 atgaaagcca tctgtaaatc ctgcgccatt agtcccggga cgctctatca ccatttcatc
    11761 tccaaagaag ccttgattca ggcgattatc ttacaggacc aggagagggc gctggcccgt
    11821 ttccgggaac cgattgaagg gattcatttt gttgactata tggtcgagtc tattgtctct
    11881 ctcacccatg aagcctttgg gcaacgggcg ctggtggtcg aaattatggc ggaagggatg
    11941 cgtaacccac aggtcgccgc catgcttaaa aataagcata tgacgatcac tgaatttgtt
    12001 gcccagcgga tgcgtgatgc ccagcaaaaa ggcgagataa gcccagacat caacacgtca
    12061 atgacttcac gtttactgct ggatctgacc tacggtgtac tggccgatat cgaagcggaa
    12121 gatctggcgc gtgaagcgtc gtttgctcag ggattacgcg cgatgattgg cggtatctta
    12181 accgcatcct gatcctctca tttcgcgggc tggtgataac tgtgcccgcg tttcatactt
    12241 taatttccct gtacaaaata caatctagcc gggatcggag aattcccccc caaaaatatt
    12301 caccgtagcc atatgtcatg agagtttatc gttcccaata tactcgaacg aacgttcggt
    12361 tgcttatttt atggcttctg tcaacgccgt tttaaagatt aatgcgatca atatcacgct
    12421 gtgggtattg cagtttttgg ttttttgatc gcggtgtcag ttctttttat ttccatttct
    12481 cttccatggg tttctcacag ataactgtgt gcaacacaga attggttaac taatcagatt
    12541 aaaggttgac cagtattttt atcttaatga ggagtccctt atgttacgtc ctgtagaaac
    12601 cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa
    12661 ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt
    12721 gccaggcagt tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt
    12781 ctggtatcag cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg
    12841 tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca
    12901 tcagggcggc tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag
    12961 tgtacgtatc accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat
    13021 ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta
    13081 tgccgggatc catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat
    13141 caccgtggtg acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt
    13201 ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg
    13261 acaaggcact agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg
    13321 ttatctctat gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct
    13381 tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag ttcctgatta accacaaacc
    13441 gttctacttt actggctttg gtcgtcatga agatgctgac ttgcgtggca aaggattcga
    13501 taacgtgctg atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg
    13561 tacctcgcat tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt
    13621 ggtgattgat gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc
    13681 gggcaacaag ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc
    13741 gcacttacag gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat
    13801 gtggagtatt gccaacgaac cggatacccg tccgcaaggt gcacgggaat atttcgcgcc
    13861 actggcggaa gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat
    13921 gttctgcgac gctcacaccg ataccatcag cgatctcttt gatgtgctgt gcctgaaccg
    13981 ttattacgga tggtatgtcc aaagcggcga tttggaaacg gcagagaagg tactggaaaa
    14041 agaacttctg gcctggcagg agaaactgca tcagccgatt atcatcaccg aatacggcgt
    14101 ggatacgtta gccgggctgc actcaatgta caccgacatg tggagtgaag agtatcagtg
    14161 tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca
    14221 ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa
    14281 gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg
    14341 ctggactggc atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa
    14401 ctctcctggc gcaccatcgt cggctacagc ctcggtgacg tcgccaataa cttcgccttc
    14461 gcaatggggg cgctcttcct attgagttac tacaccgacg tcgctggcgt cggtgccgct
    14521 gcggcgggca ccatgctgtt actggtgcgg gtattcgatg ccttcgccga cgtctttgcc
    14581 ggacgagtgg tggacagtgt gaatacccgc tggggaaaat tccgcccgtt tttactcttc
    14641 ggtactgcgc cgttaatgat cttcagcgtg ctggtattct gggtaccgac cgactggagc
    14701 catagcagca aagtagtgta tgcatatttg acctacatgg gcctcgggct ttgctacagc
    14761 ctggtgaata ttccttatgg ttcacttgct accgcgatga cccaacaacc acaatcccgc
    14821 gcccgtctgg gcgcggctcg cgggattgcc gcttcattaa cctttgtctg cctggcattt
    14881 ctgataggac cgagcattaa gaactccagc ccggaagaga tggtgtcggt ataccatttc
    14941 tggacgattg tgctggcgat tgccggaatg gtgctttact tcatctgctt caaatcgacg
    15001 cgtgagaatg tggtacgtat cgtggcgcag ccgtcattga agatcagtct gcaaaccctg
    15061 aaacggaatc gcccgctgtt tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc
    15121 ttcgcggtca gcgcctcgtc gttgttctac gtgcgctatg tgttaaatga taccgggctg
    15181 ttcactgtgc tggtactggt gcaaaacctg gttggtactg tggcatcggc accgctggtg
    15241 ccggggatgg tcgcgaggat cggtaaaaag aataccttcc tgattggcgc tttgctggga
    15301 acctgcggtt atctgctatt cttctgggtt tccgtctggt cactgccggt ggcgttggtt
    15361 gcgttggcca tcgcttcaat tggtcagggc gttactatga ctgtgatgtg ggcactggaa
    15421 gctgataccg tagaatacgg tgaatacctg accggcgtgc gaattgaagg gctcacctat
    15481 tcactattct catttacccg taaatgcggt caggcaatcg gaggttcaat tcctgccttt
    15541 attttgggat taagcggata tatcgccaat caggtgcaaa cgccggaagt tattatgggc
    15601 atccgcacat caattgcctt agtaccttgc ggatttatgc tactggcatt cgttattatc
    15661 tggttttatc cgctcacgga taaaaaattc aaagaaatcg tggttgaaat tgataatcgt
    15721 aaaaaagtgc agcagcaatt aatcagcgat atcactaatt aatattcaat aaaaataatc
    15781 agaacatcaa aggtgcaact atgagaaaaa tagtggccat ggccgttatt tgcctgacgg
    15841 ctgcctctgg ccttacctct gcttatgcgg cgcaactggc tgacgatgaa gcgggactac
    15901 gcatcagact gaaaaacgaa ttgcgcaggg ctgataagcc cagtgctggc gcgggaagag
    15961 atatttacgc atgggtacag ggaggattgc tcgatttcaa tagtggttat tattccaata
    16021 ttattggcgt tgaaggcggg gcgtattatg tttataaatt aggtgctcgt gctgatatga
    16081 gtacccggtg gtatcttgat ggtgataaaa gttttggctt tgccctgggg gcagtaaaaa
    16141 taaaacccag tgaaaatagc ctgcttaaat taggtcgctt cgggacggat tatagttatg
    16201 gtagcttacc ttatcgtatt ccgttaatgg ctggcagttc gcaacgtaca ttaccgacag
    16261 tttctgaagg agcattaggt tattgggctt taacaccaaa tattgatctg tggggaatgt
    16321 ggcgttcacg agtattttta tggactgatt caacaaccgg tattcgtgat gaaggggtgt
    16381 ataacagcca gacgggaaaa tacgataaac atcgcgcacg ttctttttta gccgccagtt
    16441 ggcatgatga taccagtcgc tattctctgg gggcatcggt acagaaagat gtttccaatc
    16501 agatacaaag tattctcgag aaaagcatac cgctcgaccc gaattatacg ttgaaagggg
    16561 agttgctcgg cttttacgcg cagctcgaag gtttaagtcg taataccagc cagcccaatg
    16621 aaacggcgtt ggttagtgga caattgacct ggaatgcgcc gtggggaagc gtatttggca
    16681 gtggtggtta tttgcgccat gcaatgaatg gtgccgtggt ggataccgac attggctatc
    16741 ccttttcatt aagtcttgat cgtaaccgtg aaggaatgca gtcctggcaa ttgggcgtca
    16801 actatcgttt aacgccgcaa tttacactga catttgcacc gattgtgact cgcggctatg
    16861 aatccagtaa acgagatgtg cggattgaag gcatgggtat cttaggtggt atgaactatc
    16921 gggtcagcga agggccgtta caagggatga atttctttct tgctgccgat aaagggcggg
    16981 aaaagcgcga tggcagtacg ctgggcgatc gcctgaatta ctgggatgtg aaaatgagta
    17041 ttcagtatga ctttatgctg aagtaagaaa taacgccgaa gagaaaaatc tccggcgttt
    17101 tggctgtgaa ttactgctgc ggaatagcgg gtacgaccgg aacatttaat gccggcattg
    17161 caaacatacc aacgaaatct tccagtggca ttttctgccc gtttaacgtt atctgaccgt
    17221 tagtatattg caggctggtg gtgatggtat tgtcctgcaa ggtggtcaga cggaacatct
    17281 gccccattgc tgatgcacct tcaacttgct gtttcgccag ttttttcgct tgatcttcct
    17341 gataaccttc cagcttcgct acctgagtca taaactcagt tgccatatcc accggaatgg
    17401 tcagtttcgc atccagagat ttaaccgaac gatctacttc ctgcgccagc gtttgcggcg
    17461 cttctttagt cgttgccgga tctttcagga acagcgacag attcagcgca ctttcaccct
    17521 gactgttttt ccagcttagc ggcgcgatag taatcaccgg atcgcctttc aacatcagcg
    17581 gcagggcgct aaagaaggct tccgtcactt tctcctgata aagttcggga ttgttggcaa
    17641 tttctggctg tgccagcagc gcctgagttt gcgcgttata ttgctggcta aactgatgcc
    17701 aggcttcacc atcaatctgg ccgactttta aagtcagctt gccgctgccc agatcctgat
    17761 tctgtacctt caggctgttt agcgagtaat ccagttggct attgatcgtt ttaccatcat
    17821 tgaccagatc cgatttaccg ctgatctcca tgccttccag cagtgccagt tctttgcctt
    17881 ccaccgaaat ggtcattttt tccagtgaca gtttttggtt tcctacgcgc tcaccaaaac
    17941 ttgccagcgt gctggaaccg tcggttttca gattattaaa ggtcaactgc actttctggt
    18001 tgtattcgtt aactgcgtct atccgaccac tttgcgcctc cccggaaagg gagatagctt
    18061 tgccgtctct gtccgcattt aactggaact cgccgccgct aaaggcgact ttttcatcct
    18121 tttgctcgta attcagtggc ttgagcgaaa tatcggaact ggaatcaccg ctgtaaccaa
    18181 tgcgcgagtt aatctcaaaa ggcgtttcac cttttgccat atcaaacagt ggtttgctta
    18241 cttcgttatt aaccagcgtg gtttgaattg atgccatcga cgggatcagg ttcagttttt
    18301 taagctgggc aagcgggaag ggaccatgat caaccgattc gttgaagatg acgctctgac
    18361 cgcttttaat ccacggattt tctttcccgg caatgggttt caccaacagt tgcaactggc
    18421 tgctgaatac gccgcgatga tagttttgat aactcacttc caggttggat tcaggagctg
    18481 tcagtttgag ttgcgcgttc gcctgcgcga ccatgtcttc gagatgggtt tcaatcttct
    18541 tgcctgtata ccatgcgccg cctgtccaga ctacgcctag cgcaacaatg acgcctaccg
    18601 ctaccagcga tttattcata atgattatcc ataaaatgaa atcaggcgga ctggccgcct
    18661 gaaggtgtta taagccttta ataagcttag caagagatgt taattttttc agtaagctct
    18721 tacagcttgt tgtaaacacg cgctaaacgg ccgtggcctt tgacagtcac cggtgattcg
    18781 ttggcggcaa taaacgctga ttcacccggt ttaagctgta actgctgaga acctttccac
    18841 aacgttgcat cgccttcgac gcagaacaaa atggcggcac tctgctggct aatggtggtt
    18901 tctttatcac taaggtcatg cagcgagaag gcaaaatcat ccactggaat cgggaagtcc
    18961 agttctgcac cttgtttcac cggctgggtc aacaactggt tagccggttt ggcttcgaat
    19021 ttcacattgg caaccagttc cggaatatca atgtacttag gcgtcagacc cgcacgcagc
    19081 acgttatcgg agttcgccat cacttccagc gccacgcctt gcaggtaagc gtgcggtgtt
    19141 tcagcgaaca ggaacatcgc ttcgccagga ttcaatttca ccacattcag taacagcggg
    19201 gagaacaggc cgctgtcttc cgggtaaaat tcagaaatta aacgaatcgt ttgccacggt
    19261 tcaccctgct gactgtcgag cgccgatttt aaaatcgcca gcgcgcggga tttttcttca
    19321 ccctgcatat tcaacaggct ggcgaacagt tcgcttaaac gttcggcatt aggctgttgt
    19381 aaaaagtgag caattgccgg atgtgcacct gcgactggct ggagtaggga gacaatctcg
    19441 gaaaactcac gaaacgcgtt catcgcaagg aaaggcgtca gcgcaaaaac cagctccggc
    19501 ttgtggttag gatctttata gttacgctcg gcggcatcca tcgggatacc tgcggcattt
    19561 tctttggcaa aaccgatttc agaattgcgt ttgtttggat gaacctgaat ggagagcggc
    19621 tgtgctgcgc ataatacttt gaacaggaaa ggcagttcgc caaagcgttt ggcaacggcc
    19681 tctccgagca gagtcgattt attactctca atcacatcac gcagtgaaac gatatctccg
    19741 gcggcattct gcacccgtga actgcttttc ggatgtgcgc ccatccacag ctcggccatc
    19801 ggctggctgg acggattttc cataccataa agttcagtca acgccgtttt gctgccccag
    19861 gcatagtttt gcactgagtt aatgagtttt tgcatgatca atccctgttt taatgtggaa
    19921 attaatcccg ctattaaagc aagaatccta cgggaagtaa cctggagccg caaaaagtcg
    19981 tactagtctc agtttttgtt aaaaaagtgt gtaggatatt gttactcgct tttaacaggg
    20041 caacggaaca cccgcccaga gcataaccaa accaggcagt aagtgagaga acaatgtcaa
    20101 acaaaccctt tcattatcag gctccttttc cactcaaaaa agatgatact gagtattacc
    20161 tgctaaccag cgaacacgtt agcgtatctg aatttgaagg gcaggagatt ttgaaagtcg
    20221 cacccgaagc gttaactctg ttggcgcgtc aggcatttca tgatgcgtca tttatgctgc
    20281 gtccggctca ccaacaacag gtggccgaca ttctgcgtga cccggaagcc agcgaaaatg
    20341 ataaatatgt ggcgctgcaa ttcctgcgca actccgacat cgcggcgaaa ggcgttctgc
    20401 caacctgtca ggataccggc accgcgatta tggttggtaa aaaagggcag cgtgtatgga
    20461 ccggtggcgg tgatgaagcg gcgctggcac gcggtgtcta taacacttat atcgaagata
    20521 atctgcgcta ctcgcaaaac gcgccgctgg atatgtataa agaggtgaat accggcacca
    20581 atctaccagc gcagatcgat ctttatgccg ttgatggcga cgagtacaaa ttcctctgta
    20641 tcgccaaagg tggcggttcg gctaacaaga cgtatctcta tcaggaaacc aaagcgttac
    20701 taacgccggg gaaactgaaa aattacctgg ttgagaagat gcgcacgctg ggtacggcgg
    20761 cctgtcctcc gtatcatatt gcgttcgtta ttggtggaac ttctgcagaa acgaacctta
    20821 aaacggtcaa actggcttcc gctaaatact atgatgaact gccaacggaa gggaatgagc
    20881 acggtcaggc gttccgcgat gtggaactgg aaaaagaatt gctgatcgaa gcgcaaaatc
    20941 ttggtctggg tgcgcagttt ggtggtaaat acttcgctca cgacatccgc gtgattcgcc
    21001 tgccacgtca cggcgcatcc tgcccggtcg gtatgggcgt ctcctgctct gctgaccgta
    21061 atatcaaagc gaagatcaac cgtcagggga tctggatcga aaaactggaa cataacccag
    21121 gcaaatatat cccggaagag ctgcgcaaag cgggagaagg cgaagcggtg cgcgttgacc
    21181 ttaaccgtcc gatgaaagag atcctcgcac agttgtcgca gtatcccgtt tctacacgct
    21241 tatcgcttaa cggcacgatt atcgtcggtc gtgatattgc tcacgccaaa ctgaaagagc
    21301 ggatggataa cggtgaaggg ctgccgcagt acattaaaga tcatccgatt tactacgcgg
    21361 gtccggccaa aacgccggaa ggttatgcct ccggttctct tggcccaacg accgccggac
    21421 ggatggattc ttatgtcgat caactgcaag cgcagggcgg aagtatgatc atgctggcga
    21481 aaggcaaccg cagccagcag gtgacggatg cctgtaaaaa acacggcggc ttctaccttg
    21541 gcagtatcgg tggtccggcc gctgtattgg cgcagggaag tattaagagc ctggaatgtg
    21601 ttgaatatcc ggaactggga atggaagcca tctggaaaat tgaagtggaa gatttcccgg
    21661 cgtttatcct tgtggatgat aaaggaaatg acttcttcca gcagatacaa ctcacacagt
    21721 gcactcgctg tgtgaaataa acagagccgc ccttcggggc ggttttttta catggcacga
    21781 aagaccaaac atttgttatc aaatagtaaa taataagtga gctaaaagtt gcttaacgaa
    21841 agcaaaacag aaagaaaaaa ttaaacaggt gaggagcagg ccatgaatac agtacgcagc
    21901 gaaaaagatt cgatgggggc gattgatgtc ccggcagata agctgtgggg cgcacaaact
    21961 caacgctcgc tggagcattt ccgcatttcg acggagaaaa tgcccacctc actgattcat
    22021 gcgctggcgc taaccaagcg cgcagcggca aaagttaatg aagatttagg cttgttgtct
    22081 gaagagaaag cgagcgccat tcggcaggcg gcggatgaag tactggcagg acagcatgac
    22141 gacgaattcc cgctggctat ctggcagacc ggctccggca cgcaaagtaa catgaacatg
    22201 aacgaagtgc tggctaaccg ggccagtgaa ttactcggcg gcgtgcgcgg gatggaacgt
    22261 aaagttcacc ctaacgacga cgtcaacaaa agccaaagtt ccaacgatgt ctttccgacg
    22321 gcgatgcacg ttgcggcgct gctggcgctg cgcaagcaac tcattccgca gcttaaaacc
    22381 ctgacacaga cgctgagtga aaaatcgcgt gcatttgccg atatcgtcaa aatcggtcga
    22441 acccacttgc aggacgccac gccgctaaca ctagggcagg agatttccgg ctgggtagcg
    22501 atgctcgagc ataatctcaa acatatcgaa tacagcctgc ctcacgtagc ggaactggct
    22561 cttggcggta cagcggtggg tactggacta aatacccatc cggagtatgc gcgtcgcgta
    22621 gcagatgaac tggcagtcat tacctgtgca ccgtttgtta ccgcgccgaa caaatttgaa
    22681 gcgcttgcga cctgtgatgc cctggttcag gcgcacggcg cgttgaaagg gttggctgcg
    22741 tcactgatga aaatcgccaa tgatgtccgc tggctggcct ctggcccgcg ctgcggaatt
    22801 ggtgaaatct caatcccgga aaatgagccg ggcagctcaa tcatgccggg gaaagtgaat
    22861 ccaacacagt gtgaggcatt aaccatgctc tgctgtcagg tgatggggaa cgacgtggcg
    22921 atcaacatgg ggggcgcttc cggtaacttt gaactgaacg tcttccgtcc aatggtgatc
    22981 cacaatttcc tgcaatcggt gcgcttgctg gcagatggca tggaaagttt taacaaacac
    23041 tgcgcagtgg gtattgaacc gaatcgtgag cgaatcaatc aattactcaa tgaatcgctg
    23101 atgctggtga ctgcgcttaa cacccacatt ggttatgaca aagccgcgga gatcgccaaa
    23161 aaagcgcata aagaagggct gaccttaaaa gctgcggccc ttgcgctggg gtatcttagc
    23221 gaagccgagt ttgatagctg ggtacggcca gaacagatgg tcggcagtat gaaagccggg
    23281 cgttaatccg caacatacag gtgcagccgt ggaatgatca aacgaagcgg ctgcgcctgt
    23341 ggtttataac ggtgctgcac attgtcggca tcgtaattta acaactcacc aacgtccggc
    23401 acgcgcgcgc cattatcccg attaatcagt gcaatcagcg gtgtagggca ggcgtgttgg
    23461 acttgttttt gatctccttt gtaccagacg cgggcaatcg gctgcacctt caccggacgt
    23521 ttgattttta acttcgcgtt ctgtggcagg gcagcgatat cctgatactc tcgctccagt
    23581 tttctttgcc actcctcgcg cgtccacggt gcgacactgc gtggtgattt caggcttttt
    23641 tccagctgtg ccaggacttc atcacgatgc aaattcttaa tgatatgttt attagcccaa
    23701 ccaaagcgta aagtggcggg gtcgtgcaga acggtgagcg tgcggtaagc attaagggtg
    23761 atcagccccg gcaaatgacg atgcacccat tcaaaacgtg ccgcggtggg gagttctgat
    23821 tcaaccgtga cgatatgctc gaacgtggtc ttgagtttat tgatgtgctg aatatgactg
    23881 accaacgctg cttgcgaaag gttatcgacc tggtaacaca acacgccagg cagacggaca
    23941 gcggccttgc tgctgcgatt ttcggactgt tgttgaataa ataaatggcg gaaatgacgc
    24001 aacgccagcg actgcgcgtc gttgccgaga tgttgtttta cctcaatacg attaagcgga
    24061 ttatgctcat cctctttttt tacctccggc aaagagaaca cgcgggcaac caatagcttg
    24121 tgttgctcaa gatgagcggc aaatgcagcc agctcttgtt ccatctggcg aaaggtagtg
    24181 ttgagtcggt ctacgagatc gtaacgcgcc ataatattaa ccactttagt tacaacatac
    24241 ttattttata gcacagtcgt ggtgacttga ccatcgtgcg cgtagtgtca ggcagaggta
    24301 aattgcggga tgttatgcca taacggccag ctaaacgaga agcgagcacc acccagttcg
    24361 ctggtgtcac aattaaccgt accgcccatt gccagtgcta tagagtggac aattgccagc
    24421 cccagcccgc agccgccggt tgagcgatcc cggctgggat cgaggcgaac aaaaggttca
    24481 aagatatgtt cgcggttttc tggggcaatc cctgggccat catcctcaac aattaatgtc
    24541 gctctattcc ccgacagtag caggctggtt tcaaccgttg aatggcagta ccgcagggcg
    24601 ttattgagca aattatccag cacgcgctcc attaagcgca tatccaacgc cgcataatgg
    24661 ccttgcgcga gcgttttaat ccgtaccgtt ttatcgggcg ttactgcctg aatgtctgcc
    24721 agatgcgttg acagccacaa cggcaggtct ggttcgctaa gatgaagctc gttttgtggg
    24781 cgatcgagtc gggcataagt cagcagctct tcaattaaag cttcaagttg actgatatca
    24841 cgattcaacg cctgggattc ggcggcgctc aggttatcgc tcatctccag tcgataacgc
    24901 aggcgcacta acggtgttcg cagttcgtga gcgataccgt caataagctg ttttttgctg
    24961 gcaatcaagg cgttgatatt gtccgccatc tggttaaatg cgataccaag tcgttcaaag
    25021 ctcgaaccct catcaaagtg gatacgctca ttgagatgcc catcgccaaa tcgttgcgcc
    25081 gctgcttcca gttttaacat atcctgccag tgcggacgca tccagataaa caccggaaag
    25141 gcgagggaaa tagcaataaa agcgatcagg gcgatatcca gcaatcgcat ctgatggaga
    25201 taataaagat aaggaacagg accaactgcc agcacgtagt ggctgcgcgg gatacgctgc
    25261 aaaaacgtgt actgatcgtc cagggcgaca atttcgccgc cacgcagtcg gtgcatggaa
    25321 atatcatcaa gatggtattt actcaatggc tcgacacgca gatcgaaaga gagatttaaa
    25381 tccatctctt tcagcgtctt accccagtcg tgtgggggga tctcacgcaa ttcgctgcgc
    25441 atcagataca gcgaactgtt catcaaatca tccagcgact gtttgcccgc gcgttcggcg
    25501 gtaaatttgt acaccaaccc aaccagcaga gacatcacaa ggaagcagac aaacaataac
    25561 aggtaaaact ggataaacag ttttttcatc gcttattccc atgcatgagg cgcaaaaaga
    25621 tagcctttgt tacgcacagt tttaatgcga taaggttctg cggcgttatc gagcagtttt
    25681 tttcttaacc gcgaaatagc cacgtccacg ctacgatcca gtccgtcata actgacgccg
    25741 cgtaaatttt tcagcaatgc atcgcggtcc atgatttgcc cggcatgggt agctaattcc
    25801 cacaataatt cgaaatcagc tgtcgagagc gagatttcag tgttagccag ggtgactacg
    25861 cggttgatgg gatcgatggt caacgtgccg aaatgcaggg ctttgtaggg agtcagagac
    25921 gtttcctgaa gacctttgct cagtgtggct tgctcattct gacgcaaatg caaacgtaaa
    25981 cgcgctagca aaacagcagg gggcgtcgtt ttgagaatat agtcgcaggc acccatttcc
    26041 agtgccagga tgtggttcat atcgctatcg agagaggtta gaagaacaat cggtccagac
    26101 cactttgcgc gtaaatcacg acaaatggtc atgccgtcct tgcctggtag catgatgtcg
    26161 agtaacacca aatccggatt ttctcgcaaa atggtttctt cggcctggtc gccgcgcggc
    26221 tctacggtaa cctgcatatc atgttttgcc aggtacgcgg caatcagtga accgacttcc
    26281 gcatcatctt ccacaaatac gatagtgttc ataacattca cggtagatat aaaaacgtca
    26341 caatacaccg cgctgttttt acttactatc aatcgtttct aaacaagttt tcatcaggta
    26401 ttctactgat gacttgttat ggaagtgtta aggtaaaaaa atggggctgg taatcaaagc
    26461 cgcgctcggt gcgctggtag tgctgttgat tggtgtttta gcaaaaacga aaaattatta
    26521 tatcgccggg ctgattccac ttttcccgac ctttgcgctt atcgcgcatt atattgttgc
    26581 cagcgaacgc ggcattgaag ccttacgcgc aaccatcatt tttagtatgt ggtcgattat
    26641 tccctatttt gtttacctgg tgtcgctgtg gtatttcacc ggaatgatgc gcttgcccgc
    26701 cgcttttgtt ggttctgttg cgtgttgggg gataagcgca tgggtgttga ttatatgctg
    26761 gataaagctg cattaacgca gatgacgacc gccatcaagt gggaaactgc gtccggtgac
    26821 aaagcaactg gtaagtaagt aatcgaccag gtcgatcact tctttctcgc caggcgcggt
    26881 tttcatcagt gatttattca gcgcctgttg tcgatattcg gcatcatcat gttcattaaa
    26941 caggatcagc gatggcgcaa tagaattcac tttcacttcc ggtgccagct tgcgggcaaa
    27001 cgagcgggtc atattatcca gtgccgcttt gcttgcagca tacgcaacat gtttgtcgct
    27061 accgcgctcc accacataat cggtaaagtg aatgatatcg ctggcggcgt gtccgtgccc
    27121 acgcagtaat ctttccagcg catggttgag caggtatggg gtattaacgt ggatctgcat
    27181 catgcaagcc agtacgtcgg ccagtggtgt acctggtttt tctgccatcc acgcactggc
    27241 gttatgcaaa atagcacgca gaccatgggt gctttttagt acttcatcgg caaacgccat
    27301 cacaccgtcg ttggtcgaaa aatcagcctg aatacactgc gcacctgcct taatcagtcc
    27361 atcaatggct ggatagtgcg tccgatagct gacaatcacc ggttgctttt gattaatgaa
    27421 atgccaggcg agggcgaggc cgatgcgacg acctccgcca gtaattaata ttggcaaggg
    27481 ctgggcttta cccatcgtta tctcctttgc tatccaacga tgggatgaac gttatcccac
    27541 cagcatccag gtcgccggaa ctgaggcaat taacagcatg ccaatcagca ccatttcctg
    27601 acggttcagt acattatcat gtgtatgcgt tttgcgcgca tacaggaaaa ccagcagccc
    27661 cggcgcatac agaacaacgg ataacagcaa gtgcattgga ccagaggcgt acaataacca
    27721 taagccataa atgcaggcac cgacacctac cgctttatgc agtggacggg ttgcgatttt
    27781 cagcaagaac gcgccgacca ggaaataggg caccagaatc atttctgagg cgatggtcag
    27841 caacgtgttg taatcggaac cggtcagcca gatgagtacc agacaaattt gcacgcagat
    27901 gttggtcaac cacagtgagg cggatggcgc agcttgtgcg ttctggcgcg caaaaatgcg
    27961 tgggaatgct ttatgagtgg cagccaggaa cggaacttct gccgccatga tggtccagct
    28021 taagtacgcg ccgcaaacgg aaacgatcaa accggcagcg atgatgattt cgccccatgg
    28081 tcccatcatt tccaccatca gaccggccat tgacgggtta cgaatttcag ccagttcagg
    28141 acgtgccacc acacccagcg aaagcagcgt taccagcaag taaacgccca gagcggagag
    28201 aaccgccagc agtgtcgctt tgccaacatc acgtttatta cgcgcacgcg cagaaacaac
    28261 cacggcacct tccacaccaa tgaaaaccca cagggtgatc agcatggtgt ttttcacctg
    28321 ttcccaaacg ggtacgccaa gtgcaagtcc ggtgaagtcg agcttgaagg tatccagttt
    28381 gaacatcatc atcgccagca caacaaacag acccagcggc aacaattttg ccagtgtcgc
    28441 caccaggtta atgctggcag cggtttgcac cccgcgcagg atcaaaaagt gaacaatcca
    28501 caataaagcc gatgcaccga cgatcgattg ccaggtatta ccatcgccga acaggcgcaa
    28561 ttccggcgtg tccgtaaaaa agcttaacgc ggaaaaaacg atcaccagat aggagacgtt
    28621 ggcgatgact gcgcacagcc agtatcccca tgcggaacaa aagccgatta gctcgccaaa
    28681 cccttcgcgg gcataggtaa agataccgcc gtcaagttcg ggacgaatgc gcgtgaggat
    28741 cagcatggca taggccagca ataaaatgcc agcgccagta ataccccagc cgatgagcag
    28801 tgctgccggg ctggcaactg ccgccatatt ttgcggcaga ctgaaaacac ccgcgcccag
    28861 cattgagctt aataccagcg cggtgagtgc gctcagtccc agtttctttt ccatcgatat
    28921 ccagtgatta aaacgtgata agaagaataa atattagggc aaaacgcata aaaatggagt
    28981 ttgccactga agcgcgggat tttacgaaga gatgtgtctg catgcaatgg tcgcggggaa
    29041 atttgtgact aaaaatgcga aaggcggcat caagccgcct tataggagta actgaatagt
    29101 tatttataca gatctgcgct gacggtcagg ttattaccac gttcctgcca ctggcgggtg
    29161 atgtggtaat acttagcacc tttcttcgcg gcacgtttcg caacctgata ggagacttcg
    29221 gtcatgttgc cgtagttgcc agagaatttg atgctgtcga acgggaccat catcgctgcg
    29281 gtcgctttgt tcagttcttc gactttagtg ccatccggga gcgtgacggt gtaacgcccg
    29341 ccttttgatg actgggtttc aaagaaacga ccgacttcag aacttggtga tgcggtagtc
    29401 gcaacacctg ggatctcaac tttcttcgcg gcttcgccac cggcagccag agctgcacgt
    29461 cctgcttcgg aatctgccgg gatcacatcc gggctctgga cgatacgttt cttagcatct
    29521 tttttataga tgaatgcagt aatacgctgg ttgccgccct ggttggcatc gatttgacga
    29581 acgatgtaga aagagtaggc acctttcgct tttgccgctt tggtgatggc atcattgact
    29641 tctggctggc tacgatagaa gccctggacg gtgaccgtgt caaacggttc aatcagaaca
    29701 gcctgatctt tcggcagttc gacaacaccg ttaattacgc gattacttgt ttcttctgct
    29761 ttttcagcat cggctttata gaggtcagcg accacacgcc agttaccgct gttaccaaaa
    29821 tcagaagtgt cgacaacata aaaagaggcg gcaccttctt tatctgcgcg acgagaaacg
    29881 gctttcaccg cttcgccaat agcattaaaa cgaccggtaa ccactacacg gtcaaaaggt
    29941 ttaaccgctg ccgcttgctc cggtgtcagt tctgttgctg cgttaacgga gaatgccgta
    30001 gcagaaagca gtgccgacgc caggagggtg ttcttaagct tcataaaaat aatccttcgc
    30061 cttgcgcaaa ccaggtactg gtattgttat taacgagaaa cgtggctgat tattgcattt
    30121 aaacggtgta actgtctgcg tcatttttca tatcacattc tttaagccaa ttttaatcct
    30181 gctcaaatga ccgtctatgc ttaaaaaaca gccgtatcag catcattact actgaagcaa
    30241 ctgaattgta taagttaatt taatgttaag tagtgattcg tgccggggcg atgtctcgtt
    30301 ttacccgacc gtcgaagaca attatcagtc tttatccggc gttctaaggt gtttatccca
    30361 ctatcacggc tgaatcgtta atattttgcg agttcacgcc gaaatactga tttttggcgc
    30421 tagatcacag gcataatttt cagtacgtta tagggcgttt gttactaatt tattttaacg
    30481 gagtaacatt tagctcgtac atgagcggct tgtgtggctc ctgacacagg caaaccatca
    30541 tcaataaaac cgatggaagg gaatatcatg cgaattggca taccaagaga acggttaacc
    30601 aatgaaaccc gtgttgcagc aacgccaaaa acagtggaac agctgctgaa actgggtttt
    30661 accgtcgcgg tagagagcgg cgcgggtcaa ctggcaagtt ttgacgataa agcgtttgtg
    30721 caagcgggcg ctgaaattgt agaagggaat agcgtctggc agtcagagat cattctgaag
    30781 gtcaatgcgc cgttagatga tgaaattgcg ttactgaatc cagggacaac gctggtgagt
    30841 tttatctggc ctgcgcagaa tccggaatta atgcaaaaac ttgcggaacg taacgtgacc
    30901 gtgatggcga tggattctgt gccgcgtatc tcacgcgcac aatcgctgga cgcactaagc
    30961 tcgatggcga acatcgccgg ttatcgcgcc attgttgaag cggcacatga atttgggcgc
    31021 ttctttaccg ggcaaattac tgcggccggg aaagtgccac cggcaaaagt gatggtgatt
    31081 ggtgcgggtg ttgcaggtct ggccgccatt ggcgcagcaa acagtctcgg cgcgattgtg
    31141 cgtgcattcg acacccgtcc ggaagtgaaa gaacaagttc aaagtatggg cgcggaattc
    31201 ctcgagctgg attttaaaga ggaagcgggc agcggcgatg gctatgccaa agtgatgtcg
    31261 gacgcgttca ttaaagcgga aatggaactc tttgccgccc aggcaaaaga ggtcgatatc
    31321 attgtcacca ccgcgcttat tccaggcaaa ccagcgccga agctaattac ccgtgaaatg
    31381 gttgactcca tgaaggcggg cagtgtgatt gtcgacctgg cagcccaaaa cggtggcaac
    31441 tgtgaataca ccgtgccggg tgaaatcttc actacggaaa atggtgtcaa agtgattggt
    31501 tataccgatc ttccgggccg tctgccgacg caatcctcac agctttacgg tactaacctc
    31561 gttaatctgc tgaaactgtt gtgcaaagag aaagacggca atatcactgt tgattttgat
    31621 gatgtggtga ttcgcggcgt gaccgtgatc cgtgcgggtg aaattacctg gccggcaccg
    31681 ccgattcagg tatcagctca gccgcaggcg gcacaaaaag cggcaccgga agtgaaaact
    31741 gaggaaaaat gtacctgctc accgtggcgt aaatacgcgt tgatggcgct ggcaatcatc
    31801 ctgttcggtt ggctgtcaag cgttgcgccg aaagagtttc ttgggcactt caccgttttc
    31861 gcgctggcct gcgttgtcgg ttattacgtg gtgtggaatg tatcgcacgc gctgcataca
    31921 ccgttgatgt cggtcaccaa cgcgatttca gggattattg ttgtcggagc actgttgcag
    31981 attggccagg gcggctgggt tagcttcctt agttttatcg cggtgcttat agccagcatt
    32041 aatattttcg gtggcttcac cgtgactcag cgcatgctga aaatgttccg caaaaattaa
    32101 ggggtaacat atgtctggag gattagttac agctgcatac attgttgccg cgatcctgtt
    32161 tatcttcagt ctggccggtc tttcgaaaca tgaaacgtct cgccagggta acaacttcgg
    32221 tatcgccggg atggcgattg cgttaatcgc aaccattttt ggaccggata cgggtaatgt
    32281 tggctggatc ttgctggcga tggtcattgg tggggcaatt ggtatccgtc tggcgaagaa
    32341 agttgaaatg accgaaatgc cagaactggt ggcgatcctg catagcttcg tgggtctggc
    32401 ggcagtgctg gttggcttta acagttatct gcatcatgac gcgggaatgg caccgattct
    32461 ggtcaatatt cacctgacgg aagtgttcct cggtatcttc atcggggcgg taacgttcac
    32521 gggttcggtg gtggcgttcg gcaaactgtg tggcaagatt tcgtctaaac cgttgatgct
    32581 gccaaaccgt cacaaaatga acctggcggc tctggtcgtt tccttcctgc tgctgattgt
    32641 atttgttcgc acggatagcg tcggcctgca agtgctggca ttgctgataa tgaccgccat
    32701 tgcgctggta ttcggctggc atttagtcgc ctccatcggt ggtgcagata tgccagtggt
    32761 ggtgtcgatg ctgaactcgt actccggctg ggcggctgcg gctgcgggct ttatgctcag
    32821 caacgacctg ctgattgtga ccggtgcgct ggtcggttct tcgggcgcta tcctttctta
    32881 cattatgtgt aaggcgatga accgttcctt tatcagcgtt attgcgggtg gtttcggcac
    32941 cgacggctct tctactggca atgatcagga agtgggcgag caccgcgaaa tcaccgcaga
    33001 agagacagcg gaactgctga aaaactccca ttcagtgatc attactccgg ggtacggcat
    33061 ggcagtcgcg caggcgcaat atcctgtcgc tgaaattacc gagaaactgc gcgctcgtgg
    33121 tatcaacgtg cgtttcggta tccacccggt tgcagggcgt ttgcctggac atatgaacgt
    33181 attgctggct gaagcaaaag taccgtatga catcgtgctg gaaatggacg agatcaacga
    33241 tgactttgct gataccgata ccgtactggt gattggtgct aacgatacgg ttaacccggc
    33301 ggcgcaggat gatccgaaga gtccgattgc tggtatgcct gtgctggaag tgtggaaagc
    33361 gcagaacgtg attgtcttta aacgttcgat gaacactggc tatgctggtg tgcaaaaccc
    33421 gttgttcttc aaggaaaaca cccacatgct gtttggtgac gccaaagcca gcgtggatgc
    33481 aatcctgaaa gctctgtaac cctgacggcc tctgcggagg ccgtcactct ttattgagat
    33541 cgcttaacag aacggcgatg ctttgacctc ccgctgtttg ttcaagcgca attttgacaa
    33601 taattgtcaa cggcacggaa agcagcatac ccaccggtcc taacagccat ccccaaaaaa
    33661 tcaacgacaa aaataccacc aatgtggaaa gccccagccc acgtcccatg atgcgcggct
    33721 ccagaatatt gccgaagacc agattaatta gcagatatcc cgccagcacc agcaacgctt
    33781 cgtagaagcc attaaacacc agtacctgag cgataggggg gattgccgcg agaactgaac
    33841 caatattagg gatgtaatta agcgcaaagg ccagcaatcc ccagacaaaa gcgaagcgaa
    33901 catcgagggc ggcgagcatc gcccaggcga ccaggccggt gatgatgctt atggctgttt
    33961 tcagcaccag ataatgagac acacagtcaa tcgcacgctg aatcgccgcc atcccttcaa
    34021 ccggacgcgc catcatttgc tgaaattttc cgggcaattg tggcacttcg agcagcataa
    34081 acagcaccgt cagcagcaat aaaaatattg atgacatggc attagataac tgcgtcaata
    34141 agttggtgag caacgtcatc gccgcgttcg gatctatgta atgcgccagt tggtcaactg
    34201 agacgtcaat ccctacacgt tgcaacaacg gctcaagagc ttgcagcggc gtcataatag
    34261 agttgcgata ttgcggtaac gtccgcgtca actcgttgag cgcagaaccc agataagcta
    34321 gcagcaacac catcgccatc acgatgatag tcatcaaaat cgacactgcc agtacacgcg
    34381 gcacacgcca gcggaccatg tgttgcacca gcgggttaag aataacagca ataaataatg
    34441 cgagaataaa cggcacgatg atctcggcgg caaaacggat accgcagaga ataatgacca
    34501 gcattcccaa cataatgacg atttttaggc cattgagcgt gatgatcggc tttgccatgc
    34561 ttgtccccgg tttttcttcc tttgctaata ataatgtctt tttcgctccg tcatcataac
    34621 gaaaatcaaa ccgctcaggt aaagaagtga aaatattttg agttaattct taagctatga
    34681 tacaaatcag gcgtgttcaa ctaccgagga caattatcat ccgcgatgac gagaagcaac
    34741 actgcggata attgtaatat tatggacaat ttgttcagga cgttattttc aacatttacc
    34801 catcttcgta cctcatccac tatcttgctc gtaggtgagc agcactggcg caacgcgctt
    34861 tagtcctttc ttctgcctaa gcttgcccta tgcgcggggc ttgaccactt tactttggtt
    34921 tcgctgaatt aagcgaaaat taaaataatt ctcttgcagg agaaggacaa tgtatattta
    34981 ttggatttta ttaggcctgg ctattgctac agaaattacc ggtacgctgt caatgaaatg
    35041 ggcgagcgtc agtgagggaa atggcggctt tattttaatg ctggtgatga tttctctgtc
    35101 gtatatattt ctctctttcg ccgttaaaaa gatcgcctta ggcgtagctt atgcgctgtg
    35161 ggaaggtatc ggtattttat ttattacctt gtttagcgtt ttgttattcg acgaaagttt
    35221 atcgctgatg aaaattgccg ggttaaccac cctggtcgcc gggattgtgt tgatcaaatc
    35281 aggtacccgt aaagcgcgta aacctgaact ggaggtgaac catggcgcag tttgaatggg
    35341 ttcacgccgc ctggctggca ttggcaatcg tgctggaaat cgttgctaac gtctttttga
    35401 aattttctga cggctttcgt cgcaaaatat ttggcttgct ctccctggcg gcggtgctgg
    35461 ctgcctttag tgcgctttct caggccgtaa aagggatcga cttgtctgtc gcttatgcat
    35521 tgtggggcgg gtttggtatt gccgccacgt tagccgcagg ttggatcttg tttggtcaac
    35581 ggttaaatcg taaaggctgg attggcctgg tcttgctgtt ggctggaatg atcatggtga
    35641 aacttgcctg atgaagacgc tgcccgcgct gtcggtcagc gtttgaacat tatttttgcg
    35701 acagttgatc cagcttgtcg cgaaaaccgg taacagaaat ggcccggtta tcggcgcgcc
    35761 agcgatcttt cgcggcagga gccgaacttt gcaccccaat taattgccag ccgtcatcgg
    35821 tatgcaacat cagaggcgaa ccgctgtcac ccggcaaggt atcgcactga tgtgacatca
    35881 ccgacgtttg cgcccagcca gtcacttcac agttttgatg actgtacaac gtatcgagat
    35941 gatcttcagg gtagcctgcc tgagtcactt tacgacctgc cgcttttaat gcggcggtaa
    36001 gcgcggcttt atctccctca aataacggca acggcgtaat gccagaaggg ggattacgca
    36061 gcacaatcaa tccgaagtcc cacggcgcgg ctgcgggagg tacaatccaa ccatccccat
    36121 ctgcttttaa ccgctttccc agtgtcggat caacgcggcc ttctatgtcg tggatctcat
    36181 agcgccaaag acctttattt gacacaaaac gcagcgctac cgccttatcc gctttaccct
    36241 taggcggcgt caatagacag tgtcctgccg ttaatgccag attgggtgca atcagcgtcg
    36301 ccgtacataa attgccgctg gccgtttcca gttgcccaac cgcatcccac ggtgattggg
    36361 tcgtgtcatt cactggcaca cgatcatcat gaccaaaaaa cagggtgctg acctcatcgt
    36421 ttgccgattt ggcaacgtct ggtttatctg caaatacaaa agcagacgtc aaactaattg
    36481 cacccaacac tacagcaatg gttgtacgca tatcacactc tggtgggtaa ttatgattat
    36541 taaaagcaaa ccctcataaa tactatagac gggacgggat gaaagtggga gtaaaatcag
    36601 ataactacag tcaggaaaga taaaaacggg cggcaatcag tgcgcataaa ataaggatga
    36661 tcagaatgaa ctcaaagcga taacggccca ccatactacc ctccgctaaa ggcggtgcta
    36721 aacgttaccc gcttagcacc gtaattttca ggcgcgaggg ggcgcgccag cattactgtt
    36781 gaaaacttac gctgcgggtt gtgcagcagg ttttgccggt tgctgatggc tgtgtttctt
    36841 ggcgtgtttc ttcgctgcct gcgctttttg ttcaggggct ttctgttcag ctttcgtatt
    36901 tttatgatgc tttttagccg cctgcgcttt ctgggcaggt gctgctttat gctgtttttt
    36961 atgatgtgtc gttttcgccg gcgctgcttt ggtggtcgtc gcagtcggag caggtgtggt
    37021 cgcagtctct gcagcaaagg cggcagaaga cagacccata gcagcggcaa caaccagagc
    37081 taatactttt ttcattgtca taccctcaat ttgttttttc atttaacccc actgcggggc
    37141 cgttgaaata actatatccc tgggaatggc agacttccgt gagtggttgg tttcagcgtg
    37201 taacgatatg tacaaacgct gaataaatta cagcattgat aataacgtat tgtgacttaa
    37261 gggaaattta gctaccaata atagtagtct tgatcgggtt aactatttta ctacttacaa
    37321 agcggtagaa taactgcgca tcagtaataa atgacacaca gcaaaatgaa tccgtttatt
    37381 tgggtactta taatcctgat gacgctagac gcgctgcggg aattggctgg cgcttcgtct
    37441 attttaggat ggctattaac gttggtttga gcaagcaata agtccggacg ggtagttacc
    37501 gcagtccggg cttatttttc aggcgtgcag acgacgatgc aaacgcgtcc cgaccagcag
    37561 agcaatgacc agcatcagag caataaatgc gccgacgccg ttccagccat agttatgcca
    37621 gaaaacacca cccagcgtcc cggcaatact cgaccccaga tagtaactga acagatacag
    37681 cgaggaggcc tggcctttag cgcgttttgc gcgggggcca atccagctgc tggcaactga
    37741 gtgggctgcg aagaatcctg ctgagaagag taacattccg gcaaagatca gccacagcga
    37801 gctgaataag gtcatcagta aaccaaacag cataaccccc gtcgaaaaca acatcaccgg
    37861 accgcgcccg tagcgggtgg tcatggttcc ggctttgggt gagctccatg taccggtcaa
    37921 ataagccagc gataataagc caaccacggc ctgactgaca tgccaggggg aaagcatcaa
    37981 ccgatagccg atgtaattaa acagcgtgac gaacgacccc atcagcaaaa agccttctgc
    38041 gaacaataac ggtaatcccc ggtcacgcca gtgcagacga aagttgataa ataacgtctt
    38101 agggcgcagc gaagtcgggc gaaaatggcg tgattccgga aggattttcc agaacatcaa
    38161 cgccgaggcc agcgcgaaac aaccgattgc cgccagagca attcgccagt tgaaaaagtc
    38221 cgtgaagaca ccactaatta agcgtccgct catgccgcca attgagttgc cgctgatata
    38281 caaccccatc gaaaaggcca cgaaactggg gtggatttcc tcgctaagat aagtcatgcc
    38341 aacagctgcc acgccactta acgaaagccc aatcaaggcg cgcataatca aaatgccgtg
    38401 ccagctggtc atcattgtcg aaagtaacgt acaaatggag gccaacagta gcgccgtgac
    38461 catcactggt ttgcgaccaa tggcatcgga tagggggcca gtaaacagca aaccaatagc
    38521 caacatcgcc gtggaaatgg acagtgaaat actactgttc gcgggcgtta agccaaactc
    38581 ctgcgaaagc accggaagga taggttgcac acaatagaga agtgcgaagg ttgccagtcc
    38641 ggcagagaac agcgccaggg tgacgcgcat aaattgcggc gtaccgcgtt taataaattg
    38701 atttggctga gaaatgcttt gcttgtcagt gtcgcttgcc ggagcgccat caacagttgt
    38761 agtacggctc acttgaaatc cttgctaaat atgcctgtgg atcaggctta tacatagggt
    38821 aggaaaatcg aattgttctg tctaatatat taataatctc aaataagatg ttttaaatat
    38881 gaatattgaa cttcgtcatc tgcgttactt tgttgctgtt gcggaagagc tgcatttcgg
    38941 gcgcgccgct gcccgcctga atatttcgca accgccgcta agtcagcaga ttcaggcgct
    39001 ggagcaacaa attggtgccc gactgctggc acgaaccaat cgcagtgtat tgctgacggc
    39061 agcaggaaaa cagtttcttg cagatagtcg gcaaatcctg tctatggtgg atgacgctgc
    39121 cgctcgcgct gaaaggctgc atcagggtga agcgggggag ttgcgtattg gttttacttc
    39181 gtccgctcct tttattcggg cggtgtccga tacgttatcg ctgtttcgcc gtgattatcc
    39241 tgatgtccat ttacaaaccc gcgaaatgaa cactcgtgag caaatcgctc cgctcattga
    39301 aggaacgctg gatatgggat tgctgcgtaa cacagcgtta ccggagacgc ttgaacacgc
    39361 agtcatcgtc catgaaccgc ttatggcgat gatcccgcac gatcatcccc tggcaaataa
    39421 cccgaatgta acgctggctg aactggcgaa agaacccttt gtcttttttg atccgcacgt
    39481 cgggacaggg ctgtatgacg atattctcgg actgatgcga cgttaccatt tgacgcccgt
    39541 catcactcag gaggtgggcg aggcaatgac catcatcggt ctggtttccg ccggtctggg
    39601 tgtttcaatt ttgcctgctt catttaaacg tgttcagctc aacgaaatgc gctgggtgcc
    39661 gattgctgaa gaggatgcgg tttctgaaat gtggttggtc tggccgaaac atcatgaaca
    39721 aagtccagct gcgcgtaact ttcgtattca tctgctgaat gctctcaggt gagggaaatt
    39781 tcagcgaaaa agcccgaaaa atgtgctgtt aatcacatgc ctaagtaaaa atttgacgac
    39841 acgtattgaa gtgcttcacc atagcctaca gattatttcg gagcgcgaaa atatagggag
    39901 tatgcggtgg ttgctgaaaa ccagcctggg cacattgatc aaataaagca gaccaacgcg
    39961 ggcgcggttt atcgcctgat tgatcagctt ggtccagtct cgcgtatcga tctttcccgt
    40021 ctggcgcaac tggctcctgc cagtatcact aaaattgtcc gtgagatgct cgaagcacac
    40081 ctggtgcaag agctggaaat caaagaagcg gggaaccgtg gccgtccggc ggtggggctg
    40141 gtggttgaaa ctgaagcctg gcactatctt tctctgcgca ttagtcgcgg ggagattttc
    40201 cttgctctgc gcgatctgag cagcaaactg gtggtggaag agtcgcagga actggcgtta
    40261 aaagatgact cgccattgct ggatcgtatc atttcccata tcgatcagtt ttttatccgc
    40321 caccagaaaa aacttgagcg tctaacttcg attgccataa ccctgccggg aattattgat
    40381 acggaaaatg gcattgtaca tcgcatgccg ttctacgagg atgtaaaaga gatgccgctc
    40441 ggcgaggctc tggagcagca taccggcgtt ccggtttata ttcagcatga tatcagcgca
    40501 tggacgatgg cagaggcctt gtttggtgcc tcacgcgggg cgcgcgatgt gattcaggtg
    40561 gttatcgatc acaacgtggg ggcgggcgtc attaccgatg gtcatctgct acacgcaggc
    40621 agcagtagcc tcgtggaaat aggccacaca caggtcgacc cgtatgggaa acgttgttat
    40681 tgcgggaatc acggctgcct cgaaaccatc gccagtgtgg acagtatact tgagctggca
    40741 cagctgcgtc tcaatcaatc catgagctcg atgttacatg ggcaaccgtt aaccgtggac
    40801 tcattgtgtc aggcggcatt gcgcggcgat ctgctggcaa aagacatcat taccggggtg
    40861 ggcgcgcatg tggggcgcat tcttgccatc atggtgaatt tatttaaccc acaaaaaata
    40921 ctgattggct caccgttaag taaagcggca gatatcctct tcccggtcat ctcagacagc
    40981 atccgtcagc aggcccttcc tgcgtatagt cagcacatta gcgttgagag tactcagttt
    41041 tctaaccagg gcacgatggc aggcgctgcg ctggtaaaag acgcgatgta taacggttct
    41101 ttgttgattc gtctgttgca gggttaacat tttttaactg ttctaccaaa atttgcgcta
    41161 tctcaatttg ggccaggaaa gcataactta gactttcaag gttaattatt ttcctggttt
    41221 atatttgtga agcataacgg tggagttagt gatgctgaag cgtttcttta ttaccggtac
    41281 agacacttct gtagggaaaa cggtggtttc ccgcgcattg ctacaagcgt tagcctccca
    41341 gggaaaaacg gttgcgggat ataaacccgt agcgaagggg agcaaagaga cacccgaagg
    41401 gctgcgtaat aaagatgccc tggtgttgca gagtgtttca accatcgaac tgccttatga
    41461 agcagttaat cctatcgcgt taagcgaaga agaaagtagc gtggcgcaca gttgcccaat
    41521 caattacacc ctcatttcaa acggcctggc aaacctgacc gataaagtcg atcatgtcgt
    41581 ggtagaaggg actggcggct ggcgcagtct gatgaatgat ttgcgtccac tctctgaatg
    41641 ggtggtgcag gaacaactgc cggtgttgat ggttgtcggt attcaggaag gttgcattaa
    41701 ccatgcactg ctaacagctc aggcgattgc caacgacggg ctgccgctca ttggctgggt
    41761 ggctaaccga atcaacccag gactggcgca ttatgcggaa atcattgatg tgctgggtaa
    41821 aaaacttccg gcaccgctca ttggtgaact gccttatctg ccgcgcgccg aacagcgaga
    41881 actggggcaa tacatccgct tagctatgct gcgcagtgtg ctggcggtag atagagtcac
    41941 ggtgtaacgt ccgcgaaatt accgacgcaa tgacgcaggc aatcaataaa ccggggagta
    42001 gctgatactc cccggtcatt tcacatatca tcaacgtcga cataatcggc gcgtgcgtgg
    42061 ttgccgccaa cagtgtcgcc atcccggtca atccgagtaa aagcgtaatt tcttcgccat
    42121 cagggaacca taatcccagg ctacgaccat acaacatgcc aatggcaaga ccgataaata
    42181 gcgtcggtgt aaagacacca ccgggtgcgc cggaaccgct actcgccagc acggcacaca
    42241 gtttacaaag gaagatcccg gcaatgatca ttaacagtgg tggggcggtt aaaaaggatt
    42301 gtacggtgct atagccgttg ccccacactg caggtgtgaa cagggaaagc agacccacga
    42361 tcaacccgcc tagtgccagt tgccagggcg gcgcaagttt gagactcaca aatccacgat
    42421 gacaggcgtt cattaacgtt aacaacagtg gtccgcacag acctgccagc acacctgtac
    42481 tgataatcag cgcatagtca cgagcctgaa ccgtcactga gagttgtacg ttgtagagta
    42541 acgcgtcgct atgattaatc agattgctaa ccagcaatgc cacgacggcg gaaataatca
    42601 ccgggccgag agaggccaac atcatagtgc caaacagcac ttcggctata aataaactgc
    42661 cagcaagcgg ggcacgatag gccgcagcca ttcccgccgc ggccccacag gcgatccata
    42721 atttccactc ctggcgtggc gtaaaacgtt gggcaaaaca ggaggcggca agggcagcta
    42781 aaagaatcat cgcaccttcg cgaccaattg cactgccgct ggttactacc agcagagagg
    42841 caagcgattt aaccaggctt gctgcgtaat cgaactgtcc atcggtttgc aacgcttcca
    42901 tgtaatcggt cggcgcatga gggcgttgtt gggtaaattt ctgccagccc atcagcaaca
    42961 aacccgccgc cagtccgccg agcgccggag ttagcaaccg tcgccagggg gaaaggtttg
    43021 tcgctgcatt gaccagactg ccggagtcat tattgaggaa caaccactcc agtagcagca
    43081 tcgcatgacg aaacccggca acggcaaagg ccgcgagaat accgacgact gttgcgataa
    43141 gcagacggcg gaacatagtg cgcagatccg gatagatgtg tagatgatgc atgagcagaa
    43201 atgggcaatg aaacacgggc gactattttg cggcaaaaga ggccagccgc aaaagcagtg
    43261 cgtgctttaa gagataaaaa aaacgtgaca ctgtaaccta ttattgccgg attgcgcgta
    43321 atcgtcacca tccggcaata ttacggtgat cctatcgaaa cagcggttta accgcgacag
    43381 gaattaacag ttgtgattgc cattgcgcca gcgttaagcg agccagttca cccagtgcac
    43441 ggtagaaagg gtgttctgct ttttcgataa aaacatcaag aaaacgtgtt gaccacggaa
    43501 aaaggtgcca tgccagcaat tcttcgcatt ctgtctggcg accattctcc gccaaccacg
    43561 cagccatcaa cagcagtgaa ccaaaatgat cttccggttc gttttgcttc atttcaaact
    43621 gaatgccttt ctcgcgcatc cactgacgaa gtgccaatgt tgaatcgcca aacagcacag
    43681 attcgcgatc cagccagacc gacccccacg gcggagacgg cagtgcccac gggccgacaa
    43741 acaaacgctg ccaggcctgg gcgtgagtct cttcacactg tgtctgaaaa gccgtcacca
    43801 gcggcgctaa tgacgcttct ggtagaggcc actgagtttc ccagccgtca ctggtgagta
    43861 ccgcaaccag aggtgcggct tccgcactct ctggagcgta ataaaacagc gcgcccaaca
    43921 cgcgcgccgc gacagaaaaa ttatcttgct gtgaaaaatg ggtcattcct aacatcctga
    43981 aatgtgcggg ttgccccgca ccaatttgtt aacctgcaat agccattcct actgtcatgt
    44041 gcaggccata aaagagcaca cgcccgatca tctcaccgcc aagtatgagg attagcccca
    44101 gaataagtcc ggcaacgtga ggttcacggc gacgaatgag cgggcaaagc cagcatccaa
    44161 gacctgcgca taacaatacc acacgccaga cctgtaagga ggcgtaatct ggtaccagtg
    44221 cgctggcttg ttgcacggat gagtgaatag acgccagaga caaaccttgc agcacaatca
    44281 ccccggcaca agcgatcagc gccagaacgc taatgatggc aaatggtgtg gtattaaatg
    44341 taacgcgggc tgcccgcaga attgcggcag caagtattgg accgctcaac aataccgtca
    44401 ggaagaaagc cagcgtagtg taaccgttat gccaggttgg cacagtgtcg atttgataca
    44461 cgcaggtcat catccagacg aaaatgacgc cgagcgccat gctgacaagt aaccagagtt
    44521 tccccaacgc ttgcggcatt ttaccgatga ccgccaccag ccaccatagt ccgccaacgg
    44581 caaaaaatat ggaaccggca gcaatctcgt tactcagacc agaagctccg attcgattca
    44641 gcgagttaaa cgcccgcagt ggcgatccca ggtgcatgac agaggcaata aacccgatgc
    44701 ccatcaacag ccagaggaaa aacatgccac ggacaatacg ctgacggtct gcgtcatttt
    44761 tggcggcgaa ccagccaatt ccgctaacaa tcagcgcgcc tactacgcat tgacccaata
    44821 cggtaaagat aaccagtggc cactcatgcc atccatttcc cattttacac ttcctccgga
    44881 ttagccagat aaccagtggt atcgccggtc gggcggctgt tggcgttagg tttgataacg
    44941 atattgggtt ttgtgaaatg cgcgcgcggc agtggcgcga cggcagccag agtgccgtgc
    45001 ttctggcgca gctcttcaat tggaccgaac tcaagtgcac gcagcgggca ggattcgaca
    45061 catatgggtt gtttcccctc ggcgacgcgc gaataacaac catcgcactt cgtcatgtgc
    45121 cctttttcag cattgtactg tggcgcgccg tacggacaag ccatgtggca gtagcggcag
    45181 ccgatacaaa catcttcatc aaccaccaca aagccatctt cacgcttatg cattgcgccg
    45241 ctggggcaga cttttgtaca tgcggggtca tcgcaatggt tacaggagat ggagagataa
    45301 taagcgaaca cgttctggtg ccagacgcca ttatcttcct gccagtcgcc gccagcgtat
    45361 tcataaatac ggcggaaact gacttccggg ccaagatctt taaaatcttt gcacgccagt
    45421 tcgcaggttt tacagccagt acagcggctg gaatcgataa aaaatccata ttgtgtggtc
    45481 atgggctact ccttaaacct tttcgatctg gacaagattg ctgtgcgacg ggtttccctt
    45541 tgccagcggt gaagggcggt gagaggtcag aatattgata ctgccgccgt gatcgacccg
    45601 gtcaccaaac atatccgctt taagccacgc accttgcccg atggcggtaa cgccaggcag
    45661 aatacgcgga gtcacttttg cggcaatcag catttctcca ttattgttaa atacccgcac
    45721 ggtatcgcca tgacggatac cgcgtgcctg agcatcaatg gggttgatcc acacctcttg
    45781 tgggcaggcc tgctgtaaca catcaatatt gccgtagctg gagtgggtac gcgctttgta
    45841 gtggaagccc gttaactgca gtggataggt tttccgcagg ggatcgtccc agccatcaaa
    45901 acctggggta tacgcaggaa ggggatgaat aatttcatct tttttcaatt cccaggtatc
    45961 tgcaatcttc gccagtcgtt ccgaataaat ttcgattttc cccgaaggtg ttttcaacgg
    46021 gtttgcctgt ggatcttcac ggaatgcgcg gaaagcgacg tagtgttctt ccgggcattt
    46081 tttcttaaag atcccggtcg ttttcatctc ctcgtagtcg ggcatctcag ggttacgttc
    46141 cttcgttttc gcatggagat atttgatcca ttcatgctga ctgcgacctt cagtaaaggt
    46201 ttgataaacg tctggtccta agcgtttggc gacttcactc agcatccagt agatgggttt
    46261 gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc acatagccca tattccctgc
    46321 agattcatga gagataaggt cttcttgctc tgttggcatc aggtcgggca acaggatatc
    46381 gcaatactta gccgaggccg tcatgaagtg gtcaatgcca acaatcatct cgcacttgct
    46441 gtcatcctga agcacctcat gggtgtgatt gatgtcgcca tgttgattga tcaatgtgtt
    46501 actggcgtag caccataaaa acttgatggg gacatccagt ttttcttttc cacgaacacc
    46561 atcacgggtc gcggtcattt ccttaccatg gtcgatggca tctgtccatg taaagacgga
    46621 aatctgcgtt ttaacagggt tctcaagcat cgggaaccat tctaccccca gatcccagct
    46681 accttcgcgt acacctgagt tgccgccgtt tatgccgacg ttaccggtga gaacggaaag
    46741 catggcaata gcgcgggacg tttgctcgcc gttggaatgt cgttgtggcc cccaaccctg
    46801 acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc aactggataa ttttttctgc
    46861 cgggatgctg gtgatttttg ctgcccattc cggcgtttta gctatgccgt caggcccttc
    46921 gcccagaata taggctttat aatgcgcgtt acgtggtgcg ttggcgggca gagttttttc
    46981 atcgtaacca acacaatatt tgtcgagaaa tggctgatcg accatgtttt cagtaatcag
    47041 tacccaggca atcgcacagg ccagtgcgcc atcggtgcct gggcgaatgg gcagccattc
    47101 atcttcacgc ccggcagctg tgtcgttata acgtggatcg atgacgatca tgcgtgcgtt
    47161 tgaacgttcg cgggcttgct cgacgtagta agtgacccca ccgccgctca tccgcgtttc
    47221 tgccgggtta tttccgaaca taacgaccag tttcgtattg gcgatatcat ccgggctgtt
    47281 gccatcattg gcaccgaaca tataactcat tgcggcactg atctgtgcgg tactgtagct
    47341 gccatagcga ctgagaaaac caccgcaaga gttcatcaga cggtacggga cgtttgagtt
    47401 ggtgatgttt ccgccatcta cgcctgttcc gtacaggaca tgtacagcct cattgccgta
    47461 atctttcagg atccgccgaa gattatcact gatggtatcc agggcttcgt cccaacttat
    47521 ccgttcaaat ttaccttcac cgcgcttgcc gacgcgcttc atgggatatt tcaacctatc
    47581 aggatgattc atccgtcggc ggatagagcg cccgcgtaaa cacgctcgaa cctgatggtt
    47641 accgtagacg tcgtcacctg tcgtatcaga ctccacccag tacacggtgt catctttcac
    47701 atgcaaacgt aacagacagc ggctcccgca gttaacggtg caggaactcc agaccgcttt
    47761 ctcttctacc ggagcctctg ccgcccggac catttgggaa aatggcagag tgaaagcact
    47821 gcttgccagc gcaagactgc caagtgcgga ggttttcatc agacttctac ggctgatttc
    47881 agccttcatg agcgcctctg tggtatggat tttcatcatt actcacttat tgcttttcaa
    47941 acaaaatgtc atgccagaat ttatggttgt cgtgggttat attttttcga tctcgaccag
    48001 attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg cgcagagtgg ttagcgtatt
    48061 cacacagccg ccatggtcga ttttatcgcc agacatattg gcctcgtgcc aggctccctg
    48121 gcccatagcg ctaactccag ggagaatacg tggtgttact ttggctggta gccgaacttc
    48181 gccacgatgg ttaaacaccc gcaccatatc gccgttggca atcccacgtt tctgcgcatc
    48241 tatagggttg atccacacct cctgacggca ggcagccttc aggagatcaa tattgccgta
    48301 ggtcgagtga gtacgggatt tgtaatggaa accaaacagt tgcagtggga aggttctacg
    48361 ttcaggggag ttccagcctt caaaggttga ggcataaact ggcaatgggc ttatcacttc
    48421 atctttttcc agttcccagg tacgggcaat ttccgccagc ctgctggaat aaatttcaat
    48481 cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg tcacgaaatg ctttgtaggc
    48541 gacaaaatgg ccattgggat ctttacgctt atagataccc atttttttca gttcgtcgta
    48601 agacggtaac gccggatctt tggcaagcat tttggcgtac agatgttgta accattgttc
    48661 ctgcgtgcga ccttctgtga acttttgata gacgtcaggt ccaagacgtt tcgcgacttc
    48721 actcaggatc cagtaaatcg gtttgcgttc gaatttttcg ctggtgacag gctggaggaa
    48781 aatgagatat cccatgttac cggcgtagtc gttaggaata atatcttcct gctcaacggt
    48841 catcaggtct ggcagcagaa tgtcggcata ttttgccgat gaggtcataa agttttcgat
    48901 gatcacaatc atttcgcatt tcgattcgtc ctgcagaatt tcatgcgttt tgttgatgtc
    48961 agaatgctga ttaacgaggg tatttcccgc gtagttccag atgaacttaa tgggcacatc
    49021 cagtttatct ttgccgcgga cgccgtcgcg gattgccgtc atttgcggac catgatcgat
    49081 agcatctgtc cagctgaagc aggagattga cgttttgacc ggattatcca gcaccggcag
    49141 gcgttctatg gtaatggtat aggtcgattc acgcgcgcca ctatttccgc cgctgatgcc
    49201 gacattgccc gtcaaaatag gtaacatagc aatagcgcgt gcagtcagtt cgccgtttgc
    49261 ctggcgttgc ggcccccagc cctggcagat ataagcgggt tttgctgtgc caatttcacg
    49321 cgccagtttg atgatacggt ctaccgggat accggtaatt tgcgaagccc actgcggcgt
    49381 tttcgctgtg ttatcatcac cttcaccaag aatataggct ttatagtgac catttttggg
    49441 tgcatctgcg ggtaaggttt tttcgtcata gccgacgcag tatttatcga gaaaaggttg
    49501 atcaacgaga ttttcgttaa tcaataccca ggcaataccc gcaaccagcg cggcatcggt
    49561 gcccgggcga atagggagcc attcatcttc acgaccagca gccgtatcgg tatatcgcgg
    49621 atcgataaca atcattttgg cgttcgattt ctcgcgcgct ttttcaagaa gataagtgat
    49681 gccaccgccg ctcatgcggg tttctgccgg gttgttacca aacatcacga ccagcttgct
    49741 gttttcaata tccgtggtgc tgttgccatc attactgccg taggtgtagg gcatggcaca
    49801 ggaaatttgc gcggtgctgt aggagccata ctgattgagt gaaccgccgt agcagttcat
    49861 caggcgtttg accgccgagg ctgatggcga agagcgggtc atattgccgc caacaatccc
    49921 cgaagagtac tgaatatata cagcctcatt gccatattgt tcgacggttt ttttcaggct
    49981 actggcgata gtatccaggg cttcatccca gctaatccgt tcgaatttgc cttcaccgcg
    50041 tgtacccacg cgtttcattg ggtaattcaa gcgatcggga tgattaatac gccggcggat
    50101 ggagcgaccg cgcaaacagg cgcgtacctg atggttgccg tactcatcgc tgccggtatt
    50161 gtcagtttcc acccaggtca cttcattatc tttaacatgt agacgaagtg cacagcggct
    50221 accacagttg acggaacagg caccccagat cactttttcg ctggcctgtt gtaccgttgc
    50281 cgctgcactg cgcagggtga acggtaaaga aaaaccgcct gcagccagcg ccagagaacc
    50341 tatcgcggtt gatttaacga gtgttctgcg gctgatgccc accattcggt catttttgga
    50401 cataactcac tccc
//
LOCUS       DAPGWD010000016        48632 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPGWD010000016 DAPGWD010000000
VERSION     DAPGWD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.016"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            102..2741
                     /locus_tag="RHX88_000882"
     CDS             102..2741
                     /locus_tag="RHX88_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDV2229609.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            2738..2923
                     /locus_tag="RHX88_000883"
     CDS             2738..2923
                     /locus_tag="RHX88_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDV2229610.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            2928..3257
                     /locus_tag="RHX88_000884"
     CDS             2928..3257
                     /locus_tag="RHX88_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDV2229611.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(3429..4334)
                     /gene="feaR"
                     /locus_tag="RHX88_000885"
     CDS             complement(3429..4334)
                     /gene="feaR"
                     /locus_tag="RHX88_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HDV2229612.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            4570..6069
                     /gene="feaB"
                     /locus_tag="RHX88_000886"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             4570..6069
                     /gene="feaB"
                     /locus_tag="RHX88_000886"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDV2229613.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            complement(6127..8400)
                     /gene="tynA"
                     /locus_tag="RHX88_000887"
     CDS             complement(6127..8400)
                     /gene="tynA"
                     /locus_tag="RHX88_000887"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDV2229614.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQSLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGAVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDYNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(8648..10693)
                     /gene="paaZ"
                     /locus_tag="RHX88_000888"
     CDS             complement(8648..10693)
                     /gene="paaZ"
                     /locus_tag="RHX88_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HDV2229615.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELLPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     AQFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            10978..11907
                     /gene="paaA"
                     /locus_tag="RHX88_000889"
     CDS             10978..11907
                     /gene="paaA"
                     /locus_tag="RHX88_000889"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HDV2229616.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            11919..12206
                     /gene="paaB"
                     /locus_tag="RHX88_000890"
     CDS             11919..12206
                     /gene="paaB"
                     /locus_tag="RHX88_000890"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HDV2229617.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            12215..12961
                     /gene="paaC"
                     /locus_tag="RHX88_000891"
     CDS             12215..12961
                     /gene="paaC"
                     /locus_tag="RHX88_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HDV2229618.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            12976..13473
                     /gene="paaD"
                     /locus_tag="RHX88_000892"
     CDS             12976..13473
                     /gene="paaD"
                     /locus_tag="RHX88_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HDV2229619.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            13481..14551
                     /gene="paaK"
                     /locus_tag="RHX88_000893"
     CDS             13481..14551
                     /gene="paaK"
                     /locus_tag="RHX88_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HDV2229620.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            14548..15315
                     /locus_tag="RHX88_000894"
     CDS             14548..15315
                     /locus_tag="RHX88_000894"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HDV2229621.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            15315..16103
                     /gene="paaB"
                     /locus_tag="RHX88_000895"
     CDS             15315..16103
                     /gene="paaB"
                     /locus_tag="RHX88_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HDV2229622.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            16105..17532
                     /gene="paaC"
                     /locus_tag="RHX88_000896"
     CDS             16105..17532
                     /gene="paaC"
                     /locus_tag="RHX88_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HDV2229623.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            17522..17944
                     /gene="paaI"
                     /locus_tag="RHX88_000897"
     CDS             17522..17944
                     /gene="paaI"
                     /locus_tag="RHX88_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HDV2229624.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            17944..19149
                     /gene="paaJ"
                     /locus_tag="RHX88_000898"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             17944..19149
                     /gene="paaJ"
                     /locus_tag="RHX88_000898"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HDV2229625.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            19176..20489
                     /gene="paaF"
                     /locus_tag="RHX88_000899"
     CDS             19176..20489
                     /gene="paaF"
                     /locus_tag="RHX88_000899"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HDV2229626.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQSQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            20590..21540
                     /gene="paaX"
                     /locus_tag="RHX88_000900"
     CDS             20590..21540
                     /gene="paaX"
                     /locus_tag="RHX88_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HDV2229627.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            21522..22112
                     /gene="paaY"
                     /locus_tag="RHX88_000901"
     CDS             21522..22112
                     /gene="paaY"
                     /locus_tag="RHX88_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDV2229628.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            22343..23203
                     /gene="pdxI"
                     /locus_tag="RHX88_000902"
     CDS             22343..23203
                     /gene="pdxI"
                     /locus_tag="RHX88_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDV2229629.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            23267..25573
                     /locus_tag="RHX88_000903"
     CDS             23267..25573
                     /locus_tag="RHX88_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HDV2229630.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGGINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLTFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            25744..26349
                     /locus_tag="RHX88_000904"
     CDS             25744..26349
                     /locus_tag="RHX88_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV2229631.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRFGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            26349..27245
                     /locus_tag="RHX88_000905"
     CDS             26349..27245
                     /locus_tag="RHX88_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV2229632.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            27261..29018
                     /locus_tag="RHX88_000906"
     CDS             27261..29018
                     /locus_tag="RHX88_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDV2229633.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            29008..30324
                     /locus_tag="RHX88_000907"
     CDS             29008..30324
                     /locus_tag="RHX88_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDV2229634.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(30375..30980)
                     /gene="azoR"
                     /locus_tag="RHX88_000908"
     CDS             complement(30375..30980)
                     /gene="azoR"
                     /locus_tag="RHX88_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDV2229635.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            31181..35083
                     /gene="hrpA"
                     /locus_tag="RHX88_000909"
     CDS             31181..35083
                     /gene="hrpA"
                     /locus_tag="RHX88_000909"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDV2229636.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTALGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEENGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTTYPISDKRILQAMEQISG"
     gene            35355..36155
                     /locus_tag="RHX88_000910"
     CDS             35355..36155
                     /locus_tag="RHX88_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDV2229637.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            36352..37791
                     /gene="aldA"
                     /locus_tag="RHX88_000911"
     CDS             36352..37791
                     /gene="aldA"
                     /locus_tag="RHX88_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDV2229638.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPTNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERIYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(37833..38834)
                     /gene="gap"
                     /locus_tag="RHX88_000912"
     CDS             complement(37833..38834)
                     /gene="gap"
                     /locus_tag="RHX88_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV2229639.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSISVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            39023..39553
                     /gene="cybB"
                     /locus_tag="RHX88_000913"
     CDS             39023..39553
                     /gene="cybB"
                     /locus_tag="RHX88_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDV2229640.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            39798..39971
                     /locus_tag="RHX88_000914"
     CDS             39798..39971
                     /locus_tag="RHX88_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229641.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(40043..40192)
                     /gene="hokB"
                     /locus_tag="RHX88_000915"
     CDS             complement(40043..40192)
                     /gene="hokB"
                     /locus_tag="RHX88_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDV2229642.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            40591..42231
                     /gene="trg"
                     /locus_tag="RHX88_000916"
     CDS             40591..42231
                     /gene="trg"
                     /locus_tag="RHX88_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDV2229643.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(42269..43192)
                     /locus_tag="RHX88_000917"
     CDS             complement(42269..43192)
                     /locus_tag="RHX88_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2229644.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     VEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            43409..44752
                     /locus_tag="RHX88_000918"
     CDS             43409..44752
                     /locus_tag="RHX88_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV2229645.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            44977..46632
                     /gene="opgD"
                     /locus_tag="RHX88_000919"
                     /gene_synonym="mdoD"
     CDS             44977..46632
                     /gene="opgD"
                     /locus_tag="RHX88_000919"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDV2229646.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            46772..46996
                     /locus_tag="RHX88_000920"
     CDS             46772..46996
                     /locus_tag="RHX88_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDV2229647.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            47059..47595
                     /gene="rimL"
                     /locus_tag="RHX88_000921"
     CDS             47059..47595
                     /gene="rimL"
                     /locus_tag="RHX88_000921"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDV2229648.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(47590..48569)
                     /locus_tag="RHX88_000922"
                     /pseudo
     CDS             complement(47590..48569)
                     /locus_tag="RHX88_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11998 a        11540 c        12892 g        12202 t
ORIGIN      
        1 ctatgcttag tgtaggcgca accttcaact gaacggttaa acatgccaca atacccgtat
       61 tgaatgctta atttttcgct aaatcaggat attaactacc catgctgggt aaatataaag
      121 ccgttctcgc gctgttatta ctgattattc ttgtgccgtt gacgctgctg atgacgctcg
      181 ggctgtgggt tcccacgctg gcgggcatct ggctaccgct cgggacacgt attgcattag
      241 atgaaagccc acgcattacg cgtaaaggtt taatcattcc cgatctccgt tatctggtgg
      301 gagattgtca gcttgcgcat atcaccaacg ccagcctttc acatcccagc cgctggttat
      361 tgaacgtcgg catggtagaa cttgattctg cttgtctggc gaaattgccg cagacggagc
      421 aatcgccagc tgctccaaaa accctcgcgc agtggcagtc catgctgcct aacacctgga
      481 tcaatatcga taaactgatt ttttctccct ggcaggaatg gcagggaaaa ctctctctcg
      541 cattaacctc tgatatccag caactgcgtt atcagggcga aaaagttaaa tttcaaggcc
      601 agctgaaagg gcaacaactt acagtcagcg aactggatgt cgtcgcgttt gaaaatcagc
      661 cgccggttaa actggtgggg gaatttacta tgccgctcgt gccggatgga cttcctgtaa
      721 gtgggcatgc tactgcgacg ttaaacttgc cgcaggaacc gtcactggtg gatgcagagc
      781 tggactggca ggaaaatagc gggcaattga ttgtgctggc acgggataac ggcgatccgt
      841 tgctcgattt gccgtggcaa attactcgtc aacaattgac cgtaagcgat ggtcgctgga
      901 gttggccgta tgcaggtttt cctttgagtg gccgactggg tgtcaaagtc gacaactggc
      961 aggcagggct tgagaacgct ctgatcagcg gacgactgag tgtgctgacc caggggcaag
     1021 cgggtaaagg caacgcggtg ctaaattttg gcccaggaaa attaagcatg gataacagtc
     1081 agctgcctct gcagctgacc ggtgaagcga aacaggcgga cctcatttta tatgcccgtt
     1141 tacctgcgca gctaagtgga agtctgtctg acccaacgct gacctttgag ccaggcgcgt
     1201 tacttcgttc aaagggaaga gtcatcgatt cgctggacat cgatgaaatc cgctggcctt
     1261 tagcgggtgt aaaagtcacc caacgtggtg ttgacggacg tttgcaggcc attttgcagg
     1321 cgcatgaaaa tgaactgggc gatttcgtgc tgcatatgga tgggctggcg aatgattttc
     1381 tccctgacgc gggccgctgg cagtggcgct actggggaaa aggcagtttt acaccgatga
     1441 atgccacctg ggatgtcgca ggaaaaggtg aatggcatga cagcacgatt acgctgaccg
     1501 acctctccac cggtttcgac cagttacaat acggtacgat gacggtagaa aagccgcgat
     1561 taattctcga caagcccgtc gtctgggtac gtgacgcaca gcatccctcc ttcagcggcg
     1621 cgctgtcact ggacgccggg caaacgctgt tcactggcgg cagtgtgtta ccgccatcaa
     1681 ccttaaaatt tagcgtcgat gggcgcgatc cgacgtattt cctctttaaa ggcgatttac
     1741 atgctggtga gattggcccg gttcgggtaa atggtcgctg ggacggtatt cgtctgcgcg
     1801 gtaacgcctg gtggccaaaa caatcactga ctgtattcca gccgctggtg ccacccgact
     1861 ggaagatgaa cttacgcgat ggtgaactgt atgctcaggt tgcattttct gctgcgcctg
     1921 aacaaggatt ccgcgcggga ggacacggcg tgttgaaagg cggtagtgcc tggatgccag
     1981 ataatcaggt taacggtgtc gattttgtcc tgcctttccg ttttgccgat ggagcctggc
     2041 atctggggac tcgcggcccc gttacgttgc gaattgccga agtgattaat ctggtgacag
     2101 cgaaaaatat tacggctgat ttgcaagggc gttatccgtg gactgaagaa gaacccttgc
     2161 tgttgactga tgttagcgtc gatgtgttag gcggtaacgt actgatgaaa caattacgta
     2221 tgccgcaaca tgacccggcg ctgttgcggc tgaataatct ctcatccagc gaactggtta
     2281 gcgccgtcaa tccgaaacaa ttcgccatgt ccggggcatt tagtggtgca ttgccgttat
     2341 ggctaaacaa cgaaaaatgg atagtgaaag atggttggct ggcaaatagc gggccaatga
     2401 ccttgcggct ggataaagac accgcagatg cggtggtgaa agacaatatg actgcgggtt
     2461 cagcaattaa ctggttgcgc tatatggaaa ttagccggtc atcgacaaaa attaacttag
     2521 ataatctcgg tttattaacc atgcaggcca acattacagg taccagtcgc gttgatggta
     2581 aaagcggtac ggtaaacctt aattactatc atgaagagaa tatttttacg ctgtggcgca
     2641 gtttacgctt tggcgataat ctccaggcat ggttggagca gaacgcacgt ctgccgggaa
     2701 atgactgtcc gcaaggaaaa gagtgtgagg aaaaacaatg aaaattttac tggctgcgtt
     2761 gacgtcatct tttatgctgg ctgcctgtac gccgcgtatt gaagtcgctg cgcccaagga
     2821 accgatcact atcaatatga acgttaaaat tgagcatgag atcatcatca aggcagacaa
     2881 agatgtcgaa gagctgcttg aaactcgtag cgatcttttc tgaggcgatg atgaagaaaa
     2941 cattacttct ttgtgcgttt cttgttgggc tggtaagcag caatgtaatg gcattgactc
     3001 tggatgaagc cagaactcag gggcgggtag gtgaaacatt ttatggttat ctggttgcgc
     3061 tgaaaacgga tgctgaaaca gagaaattag taaccgacat taatgccgaa cgtaaagcga
     3121 gttaccaaca actggcaaag caaaataatg tgtctgtaga tgatatcgcg aaactcgccg
     3181 ggcaaaagct cgtagctcgg gccaaaccgg gggaatatgt acaagggata aacggtaaat
     3241 gggtgcgaaa attttaatag caaaaccgca atatcgggat aacgctacag gagaaagcga
     3301 ttgatctatt ttcctgaaac aaggtgaata ttcaaaaact cctgtcaaat tgcctttcgc
     3361 cctgaaaaat gcatagtcaa atatctgttt taactaattg gcgttgcagt acacacaacg
     3421 ccaattagtt agcggaattt acgtcgatac tcgcctggcg tcatcccaaa gcgttgctta
     3481 aataccgttg aaaaatgact ctggtcagaa aatccccaat gaaagccgat gcctgccagt
     3541 ttttcatcat ctgcggcatg gcgaatcgca tctgcacaaa aatcgagacg acggttacga
     3601 atatattgcg cgactaccaa acctttatcg gcaaacattc ggtacaaact acgtactgac
     3661 atacctgtct ctccggctat ccactccggg cgtaatatct cttcgcgaat attatcgtct
     3721 atcaacgtaa ccactttttg aaactgacgt tcacgacgag gttgaacaga ttcccgctga
     3781 tgaagtaccg ggcgcagcag acacaccatc gcctgtagcg cagcttcact ttctgtttca
     3841 gaaagtgccg gattattcat gctctcctgt aacaggcgat gactgagttg caccatgggt
     3901 aagtcagcgt ccagtctttc tgcgcagaca ggtttttgat ggggaaaata ttgttccagc
     3961 agagtgcgtg gcaaaagtaa tgaaatctgt ttagaagact cctgccagta aagcgaacag
     4021 gggcgtgagg catcgagtaa cgtaatatcg ccagcgccaa tctgcacctg acgctcatcc
     4081 tgctccatta ttgcctgacc actaagctga aaaacggtgt aaaaccaggc atcgtcgctg
     4141 ccttttactt cctgccaggt gcgggataaa ttcaccccgc tggttgtcac ggtactcagc
     4201 tttagtcctt tggcaaaatg cgtgtccagt acacccgtgt aacgctcagt cagcaggcgt
     4261 ccggtaaaat ttccgcatac ctgattgatt tgggaaagcc attgctgaaa ctcattatcc
     4321 actgcggggt tcatggcacg ttttcgctct gtgaaatgta tttttattgt tgcatttgtg
     4381 ttgcaataaa cgaagctaat gagcctgact ataggaaata agtcttgtca ggcatagaga
     4441 cataagcggt tattgtcacg atttgcggag cttgtcacag ctgacaaagc gaatgtcaca
     4501 gcgaaaaaag tgacttttct tgtcgctgcg tacactgaaa tcacactggg taaataataa
     4561 ggaaaagtga tgacagagcc gcatgtagca gtattaagcc aggtccaaca gtttctcgat
     4621 cgtcaacacg gtctttatat tgatggtcgt ccaggccccg cacaaagtga aaaacgattg
     4681 gcgatctttg atccggccac cgggcaagaa attgcgtcta ctgctgatgc caacgaagcg
     4741 gatgtagata acgcagtcat gtctgcctgg cgggcctttg tctcgcgtcg ctgggccggg
     4801 cgattacccg cagagcgtga acgtattctg ctacgttttg ccgatctggt ggaacagcac
     4861 agtgaggagc tggcgcaact ggaaaccctg gagcaaggca agtcaattgc catttcccgt
     4921 gcttttgaag tgggctgtac gctgaactgg atgcgttata ccgccgggtt aacgaccaaa
     4981 atcgcgggta aaacgctgga cttgtcgatt cccttacccc agggggcgcg ttatcaggcc
     5041 tggacgcgta aagagccggt tggcgtagtg gcgggaattg tgccatggaa ctttccgttg
     5101 atgattggta tgtggaaggt gatgccagca ctggcagcag gctgttcaat cgtgattaag
     5161 ccttcggaaa ccacgccact gacgatgttg cgcgtggcgg aactggccag cgaggctggt
     5221 atccctgatg gcgtttttaa tgtggtcacc gggtcaggtg ctgtatgcgg cgcggccctg
     5281 acgtcacatc ctcatgttgc gaaaatcagt tttaccggtt caaccgcgac gggaaaaggt
     5341 attgccagaa ctgctgctga tcacttaacg cgtgtaacgc tggaactggg cggtaaaaac
     5401 ccggcaattg tattaaaaga tgctgatccg caatgggtta ttgaaggctt gatgaccgga
     5461 agcttcctga atcaagggca agtatgcgcc gccagttcgc gaatttatat tgaagcgccg
     5521 ttgtttgaca cgctggttag tggatttgag caggcggtaa aatcgttgca agtgggaccg
     5581 gggatgtcac ctgttgcaca gattaaccct ttggtttctc gtgcgcactg cgacaaagtg
     5641 tgttcattcc tcgacgatgc gcaggcacag caagcagagc tgattcgcgg gtcgaatgga
     5701 ccagccggag aggggtatta tgttgcgcca acgctggtgg taaatcccga tgctaaattg
     5761 cgcttaactc gtgaagaggt gtttggtccg gtggtaaacc tggtgcgagt agcggatgga
     5821 gaagaggcgt tacaactggc aaacgacacg gaatatggct taactgccag tgtctggacg
     5881 caaaatctct cccaggctct ggaatatagc gatcgcttac aggcagggac ggtgtgggta
     5941 aacagccata ccttaattga cgctaactta ccgtttggtg ggatgaagca gtcaggaacg
     6001 ggccgtgatt ttggccccga ctggctggac ggttggtgtg aaactaagtc ggtgtgtgta
     6061 cggtattaat ctggttcgct cataagtaaa aaacggcacc tggtgccgtt tttttgtctg
     6121 aaacaatcac ttatctttct tcagcgcccc tagcgttggc gtttcgtcaa agaagttcca
     6181 tggtttaagc agagtatgta cccattcggt cggcataatc ggccactctt cggcgcgggc
     6241 cacatgtgtg gtgccggtgg tcatccagac aacggcgtcg gtgttgtcca gcgactcgtt
     6301 atctttactg tattgtccaa gaccggtgtc atgagtagaa cggttcggat atttgccttc
     6361 cgggaaacgc tcgccaggat gataacgcgt tacccaaagc tgcttgtcca taaagcttaa
     6421 acgatgatag atccactcgt ccggcgcgaa ctgggcacct tttgctaccg ggtgagtacc
     6481 acctgcataa ggaataattt gataggaaac tggattgccc atgcggttct ctttgttcgg
     6541 gttactcaac agacgaatcg tgcccggatc aaacttctgc gcggcatcct gttcattgcc
     6601 gatgttgtac tgattaactt gcatggtact ggtgcgtggg ccaccggcag tattcggttt
     6661 taccaccggg tccatcgcca ccaggctgtt attctcgcca tctacatcca gatcgaggcg
     6721 gaaattataa atatgttggt gtgtagtacc cacgatattg taatcgataa gcgtgccgta
     6781 gcgcgtgtca tctttcgccg tctcatcgtg catggttttc gctttaacac ctttcaccgc
     6841 ttcgatgccc gtagcaccgg catcaatgcc aatagtgccg ttttcatgga agatccagtc
     6901 aaaaatgtag tcatagttac ccactgtact gatccagcgc accaccaact cccggcgttc
     6961 ggtactgacg ttgggctggc ccatttcctg atgcttatac tccggcccgg cataacgttc
     7021 aaataccgcg atagcacgag ggatctccat cggtacgcca gtgtagtcgg cgatggtttc
     7081 attcaggagc actgcgttag agggtgcatc tttgccacgt gcaattggcg aggttagcgt
     7141 gcccataccg tagtcaccag agtccagata cgctttaaag taccagccaa tatcaggatc
     7201 accgtaaggc acaatcatgc cgccgagaga accttcgtac atgactttgc gtttggtgcc
     7261 attgtcgtta taagtcacgg tggagatcat aggtccgacg cgcgagttca tgctgaggtg
     7321 aaaatcccag ttccgccagt gaatcatatc gccagtaatg gtgtaatttt taccttcagg
     7381 ctcaatgatt tgcataggct taactgccgg agcaacgcgg tcacggccat caaatgggcg
     7441 tgcggtcatt ggcaccggaa ctaccgcacc ttcttcaatc ttaacgattt ttttctgttc
     7501 taaatcaacg accgccacca ggttttcgat gggatgtgcc cagtagttgc catcaccgac
     7561 atcaagatag ctgatgactt tgagcaaccg ggcatcttgt ttcaggccat ctttaccatc
     7621 gaaataacct acggtcagcg gcgtggtaat cactttcttc gcatcagtaa taccgcgctt
     7681 cttcacggca gcggcaaatt cttcactgtt gttaataatg ttctgcacac tggcgaaatc
     7741 atccagcaac accataccgt gggcgtcttt aatgggttgc caggagagca gtttgttgtt
     7801 ttgcagatcc accaccgctt cgatgatatg tttgccgtcg agcataatga cgtcggcttt
     7861 gcgtggctgg tcaaccggtt tgttttccag cgcaaacgcc cagacagctt ctttatctgg
     7921 cggtagcagg gagatctcag taaaacgggt attgggtttg aagtccgcgg aagctttaac
     7981 aatttcaacg gcctgtttaa tttcgtccgc agttagcgca ttaagtgggt gagggcgctt
     8041 ttctacctga aaggtttgat ccagcccgga ctggaaaaca tcgttaataa aggtgtcaga
     8101 aacccaggct ttattgtctt tcatcactac cggtacttgc agtgccagag actgaccatt
     8161 aacaattgct gtttgcgcac caggcttcac tttcacgtac gcgccatctt taatcagggt
     8221 aaagatctgg gcgtagtcgt cccactgcac atcggcacca aattctttaa gcgttttatc
     8281 cattggcacc atatgcgctt caccaccgtg ggcaaatacc ggcgcttgcc aggcgaaact
     8341 taaggcgact gccaacgcca gggctgtttt acgggcagaa tacagagagg ggcttcccat
     8401 tattaacctc gtcagatgtt gtgttcttgt tagcaaccgc gctctgtggg cggtttagtc
     8461 aggttcacat tatcagtact gatgtaaagg ggattgcctg cacctgccag gttgtttggc
     8521 aggtgtgcca gcttttcata cagtgggtgc cctgaaaata cagatacaca tcatgcataa
     8581 cgtgacaacg tcacaaaatt tggtgaaata aaagggcaac tattcgccgt tgcccttcat
     8641 tcaccgatta atcgacaaaa tcaccgtgct gcctggccac cagcgtcaga attgaataca
     8701 gcgccaccgg ggtttgatgc tgattgaata cctctaccgc ccattccacc acacctgttg
     8761 gtttttcttc tgcgctacgc tgttttttca gcgtcttgcg cttacaggtg agacgcacct
     8821 ggatggtatc gcctggcttt acgggttcga taaagcgcaa gttttccagc ccataattag
     8881 caatgaccgg accgacaccg gcatcgacaa acagacccgc agccgcagaa agcacaaaat
     8941 acccatgcac cacccgctca ccgaaaatag attcggcagc agcaatctta tccatatgtg
     9001 catagaaatg atcgccgctg aggcaagcaa agttaacaat atcggcctct gtcattgtgc
     9061 ggcggggagt caacaggctg tcgcctggtt gtagctcctc aaaatatttg cggaacggat
     9121 gaatacgatc ttcttcgact ttcgcaccgc gcacccactg tttactgata gcggcaagca
     9181 tcgtcggact accctgaaca gcggttcgct gcatgtaatg tttcaccgct cgtaaaccgc
     9241 ctaattcttc accgcctcct gcgcgaccag gcccaccatg taccagttgt ggcagtgggg
     9301 agccatgccc ggtggattct tttgccgatt cttcattgag gatctgaatt cgcccatgcg
     9361 tacgtgccgc gtcggcaata aactgacgcg caatttgcgg atcagccgtc accagcgttc
     9421 ccgcaaggct accgccgcct gcacaagcca gttgcagagc atgttgctgg ttttgtgctg
     9481 gcattagcgt tgcgacaggg ccaaaggctt ctgttgcatg taccgccggt gtttcatccg
     9541 gctgcggaca gtacaataag gttggcggga agaaggcacc cgcagcagat aaatccgcct
     9601 gaccaccgag gcgaatctcg catcctgcag ccagcaatat gttcactttt tcctgcacat
     9661 cggcacgctg ctcagcattt accagtgcgc ccattttcac gccttcctga gcaggatcac
     9721 cgaccacgac tttctgtaat cgcgcaacca gagcatcact gacagcatta accaatgcct
     9781 gcggcacaat aatccgccgg attgccgtac atttttgccc ggcttttgtg gtcatctcac
     9841 gcacaacttc acgaataaac tgcgcaaact ccggttgatc cggggtgaca tcttcgccca
     9901 gtacgcagca gttcagggaa tcagcttcca tagtgaaggg gatagatttg gcgacgatat
     9961 ttggctgaac tcgcagcatc tgtccggtcg ccgctgaccc cgtgaaagtc accacatcct
    10021 ggctgtccag atgatccaaa aggtcgcccg cactaccgca gatcagacta attgcgcctt
    10081 cgggaacaag accactatcg acaattgatt tcaccatcgc ctgagtcagt tgggccgtcg
    10141 cggtagctgg tttgatgatg gctggcattc cgcccagcca cgttggtgcc agcttttcca
    10201 gcattcccca gcaggggaag ttaaaggcgt taatatgcac tgccacgcct gactttgagg
    10261 tcagtacatg gcgcgcggca aatccacctt ctttcgataa ggggagcaat tcatcttccg
    10321 gccacagcgt atcgtcaggc agctcccggc taccgaggct ggcgtaagta aataacgtcc
    10381 caatgccacc ttcaatatca acccaactgt ctgcccgcgt tgcgcctgtt tgcgcagaaa
    10441 gagcatagaa gcgctctttt tcactcagca gatgtttagc gaccgcttta agcatcgccg
    10501 cacgttcgat aaaggtcata gcgcgaaggg ctggggcacc tttttcaatg gcaaactggc
    10561 gggcagccgc catatcaaga ccttcactgg tcacttccca taacgcctcg ccgctaatag
    10621 cgtggtgaat caaacggcta cggccccggc cagactgcca ggtaccggat aagaaactgg
    10681 ctaactgctg catcgctact ctccagatgt ttcacatttc tgttgctaat agttaaatcg
    10741 cgaatcataa aaagcaaggg atcttttaac gaaatgttaa ctatgcgatc tgtatagcaa
    10801 ctgcggaaaa cattaatgca ctgataaata atgatttata aaaatagggt gcgaaatccg
    10861 tcacagttca aacatacaaa atttgtgatt ttagttaacc attgtgtaac tttcataaaa
    10921 caacgtgatt cgtgttttta attaattcac gaaaactgga atcgtaaagg tgatgacgtg
    10981 acccaagaag aacgctttga gcaacggata gcccaggaaa cggctatcga gccgcaggac
    11041 tggatgcccg atgcttaccg aaagacactg atccgccaga ttgggcagca tgcgcactcc
    11101 gagattgttg gtatgttgcc tgaaggtaac tggatcactc gcgcaccaac cttgcggcgt
    11161 aaagccattc tgttggccaa agtgcaggat gaagccggtc atggtctgta tctctatagc
    11221 gccgccgaaa cactgggctg cgccagagaa gacatctacc aaaaaatgct cgacggacgg
    11281 atgaaatatt cctccatctt taattatccg acattgagtt gggctgatat cggtgttatc
    11341 ggctggctgg tggatggcgc agcgatcgtc aatcaggtgg cgttatgccg cacttcttat
    11401 ggcccgtatg ccagagcaat ggtgaaaatc tgcaaagaag agagttttca ccagcgtcag
    11461 gggtttgaag cctgcatggc actggcgcag ggcagcgaag ctcaaaagca gatgttgcaa
    11521 gacgccatta accgtttctg gtggccagcc ttaatgatgt tcgggccaaa cgatgataac
    11581 tcgccaaaca gcgccagaag tctcgcctgg aaaatcaaac gtttcaccaa cgacgaactc
    11641 cgccagcgtt ttgtggataa caccgttcca caggttgaaa tgctcggtat gaccgttcct
    11701 gacccggatc tgcattttga cactgaaagc ggtcactacc gctttggtga gatcgactgg
    11761 caggagttta acgaagtaat taacggtcgc ggaatttgta atcaggagag gctcgacgcc
    11821 aaacgtaaag cctgggaaga aggtacctgg gtacgggaag cagcgctggc ccatgcacaa
    11881 aaacaacttg cccgtaaggt cgcataagga gattcaaaat gagtaatgtt tactggccgt
    11941 tatacgaagt tttcgtgcgt ggcaaacagg gcttatcaca ccgccatgtt ggcagtttac
    12001 atgctgccga tgagcggatg gcactggaaa atgcccgtga tgcttacacc cgtcgtagcg
    12061 aaggatgttc aatttgggtg gtgaaggcga gtgaaattgt tgcctcgcaa ccggaagaac
    12121 gcggtgaatt ttttgatccg gctgaaagca aggtctatcg ccatccaacg ttttacacca
    12181 tccctgatgg cattgagcac atgtgaggtc ggaaatgaat cagttaacgg cttacacctt
    12241 gcgcctgggc gataactgtc tggtgctctc ccagcggttg ggggaatggt gcggtcacgc
    12301 accggaactg gaaatcgatc tcgcactggc aaacattggc ctcgatttat taggtcaggc
    12361 acgtaacttc ttatcgtatg ccgctgaatt agcgggagaa ggtgatgaag ataccctggc
    12421 cttcacccga gacgagcgcc agttcagcaa cttattgttg gttgaacaac caaacggcaa
    12481 ttttgccgac accattgcac gccagtattt catcgatgca tggcatgtgg cgctctttac
    12541 ccgtctgatg gaaagccgtg atccgcaact ggcggcgatt tctgccaaag caattaaaga
    12601 agcgcgttat cacctgcgtt ttagtcgtgg ctggctggag cgactgggca atggtactga
    12661 cgtatcaggg caaaagatgc agcaggccat caacaagttg tggcgtttta ccgccgaact
    12721 gttcgatgcc gacgagattg atattgcact gagtgaagag ggtattgcgg ttgatccacg
    12781 cactttacgc gcagcgtggg aagccgaagt ttttgccggg atcaacgaag ccacattgaa
    12841 cgtaccgcaa gagcaggcgt atcgcactgg cggtaaaaaa ggactgcata ccgaacacct
    12901 tggacccatg ctggcagaaa tgcagtatct ccagcgtgtc ttgcccggtc agcaatggta
    12961 acagaggaga tgggtatgca acgtctggcc accattgcac cgccacaggt tcatgagata
    13021 tgggcgctac tcagccagat cccggacccg gagatcccgg tgctgaccat taccgattta
    13081 ggcatggtgc gtaatgtgac acagatggga gaaggatggg tgatcggctt tacgccgact
    13141 tattccggtt gtccggcaac ggaacatttg attggcgcga tacgtgaggc aatgacaacc
    13201 cacgggttta cccccgttca ggttgtgctg caactcgacc cggcatggac caccgactgg
    13261 atgacccccg atgcccgtga gcgtctgcga cagtatggca ttagcccgcc cgccggacac
    13321 agttgccatg cccatttgcc gccagaagta cgttgcccgc gctgcgccag cgtccatacc
    13381 acacttatca gtgaatttgg ttccacggcc tgcaaagcat tgtatcgctg cgatagttgc
    13441 cgcgaacctt tcgattattt caaatgtatt tgaggatgcc atgacaacgt ttcattcctt
    13501 aacggtggca aaagtggagc cggaaacccg tgatgcggtg accattacct ttgcggtgcc
    13561 ccagcctttg caggaggcgt atcgctttcg ccccggtcaa catttgacct taaaagccag
    13621 ctttgatggt gaagaattac gccgttgtta ctccatttgc cgcagctatc tgcctggcga
    13681 aattagtgtg gcggtgaaag ccattgaagg cggacgtttc tcccgctatg cccgcgaaca
    13741 catccgccag ggtatgacgc tggaggtcat ggtgccgcag gggcatttcg gctatcagcc
    13801 acaggccgaa cgccagggcc gctatctggc tattgcagca ggatcaggta ttacgccaat
    13861 gctggcgatt atcgccacca ctttacaaac cgagcctgaa agtcagttca ccctgatcta
    13921 cggtaaccgt accagccaga gcatgatgtt tcgccaggca ctggcagacc tgaaagacaa
    13981 atatcctcag cgtttacagt tgttgtgcat tttcagtcag gaaaccctcg acagcgatct
    14041 gcttcacggg cgtattgacg gtgaaaaatt acagtcactt ggggcctcgc tcattaattt
    14101 tcgtctttat gatgaggcat ttatttgtgg tccggcggcg atgatggatg agacggaagc
    14161 cgccttaaaa gcactgggaa tgccagataa aaccattcat ctggagcggt ttaatacgcc
    14221 tggcacgcgc gtcaaacgta gcgttaacgt gcaaagtgac ggacaaaaag tgactgtacg
    14281 tcaggatggg cgggatcggg aaatcgtgct taatgccgac gatgaaagca ttctcgatgc
    14341 ggcattgcgc cagggggctg atctgcccta tgcctgcaaa ggcggcgtct gtgcgacctg
    14401 caaatgcaaa gtgctgcgtg gcaaagtggc gatggaaacc aattacagtc tggaaccgga
    14461 tgaactggcc gcaggctatg tgttgagttg ccaggcactg ccgctgacca gcgatgtggt
    14521 ggttgacttt gacgcgaagg ggatggcatg agcgaactga tcgtcagccg tcagcaacaa
    14581 gtattgttgc tgacccttaa ccgtcccgcc gcacgtaatg cgctaaataa tgcccttctg
    14641 acgcaactgg taaatgaact ggaagctgcg gctaccgata gcagcatttc ggtctgtgtg
    14701 attaccggta atgcacgctt ttttgccgct ggggccgatc tcaacgaaat ggcagaaaaa
    14761 gatctcgcgg ccaccttaaa cgatacacgc ccgcagctat gggcgcgatt gcaggccttc
    14821 aacaaacctc tcatcgcagc cgtcaacggt tacgcgcttg gtgcgggttg cgaactggca
    14881 ttgttgtgcg atgtggtggt tgccggagag aacgcgcgtt ttggtttgcc ggaaatcact
    14941 ctcggcatca tgccaggcgc aggaggaacg caacgtttaa tccgtagtgt cggtaaatcg
    15001 ttagccagca aaatggtgct gagcggagaa agtatcaccg ctcagcaagc acagcaggcc
    15061 gggctggtta gcgacgtctt ccccagcgat ttaaccctcg aatacgcctt acagctggcg
    15121 tcgaaaatgg cacgtcactc gccgctggcc ttacaagcgg caaagcaagc gctgcgccag
    15181 tcgcaggaag tggctttgca agccggactt gcccaggagc gacagttatt caccttgctg
    15241 gcggcaacag aagatcgtca tgaaggcatc tccgctttct tgcaaaaacg cacgcccgac
    15301 tttaaaggac gctaatgatg gaattcatcc tcagtcatgt agaaaagggc gtgatgacac
    15361 taacgctcaa ccgcccggaa cgcctgaaca gttttaatga tgagatgcac gcacaactgg
    15421 cagagtgcct gaaacaggtc gagcgcgacg acactatccg ttgcctgtta cttacaggtg
    15481 ccgggcgcgg attttgtgct ggtcaggatc ttaacgatcg taacgttgat cccacaggcc
    15541 ccgcaccgga tttagggatg tcagttgaac gtttctataa cccactggta cgtcgcctgg
    15601 caaaactgcc aaaaccggtg atctgtgcag tcaatggcgt ggcggcaggg gctggcgcaa
    15661 cactggcact gggatgcgac atcgttattg ctgcccgttc agcaaaattc gtcatggcgt
    15721 ttagtaagtt aggtttagta cccgattgcg gtggaacctg gttactgcca cgcgttgctg
    15781 ggcgagcgcg cgccatgggg ctggcactgc tggggaatca actgagtgct gaacaggcgc
    15841 acgaatgggg gatgatctgg caggttgttg atgatgaaac gctggcagat accgcgcaac
    15901 agctggcacg gcatctggcg acacaaccga catttggtct tggacttatc aagcaagcga
    15961 taaatagcgc tgaaaccaat acgctcgata cgcaactgga tctggaacgt gactatcagc
    16021 gacttgccgg acggagcgca gattatcgtg aaggtgtcag tgcgttcctg gctaaacgct
    16081 caccgcagtt cacggggaaa tagcatgatg ataaatgtgc aaactgtggc agtgattggg
    16141 agcggcacca tgggggcagg cattgctgaa gttgccgcca gtcatggaca tcaggtttta
    16201 ctgtatgaca tttctgctga agcgctgacc cgcgcaatcg acgggataca cgcgcggcta
    16261 aattcacgcg tgacgcgggg aaaactgact gctgaaacct gtgaacgcac attgaaacgc
    16321 ctgatcccgg tgaccgatat tcacgcgctg gcagcagcga atctggttat tgaagcggcg
    16381 tctgaacgtc tggaagttaa aaaagcgctc tttgcacaac tggcggaagt ttgcccgcca
    16441 caaacgctat tgaccactaa cacttcgtca atctctataa ccgcgattgc tgcggaggta
    16501 aaaaatcctg aacgtgttgc ggggctgcat ttttttaacc cggcaccggt gatgaagttg
    16561 gtggaggtgg tcagtgggct ggcaacggca gcggaagttg tcgagcagtt gtgtgaactg
    16621 acattgagtt ggggtaagca gccagtgcgc tgtcattcga cccccggatt tatcgttaac
    16681 cgtgtggcgc gtccttatta ttccgaggcc tggcgggcac tggaagagca ggttgctgca
    16741 ccagaagtga ttgacgctgc acttcgcgat ggcgctggtt tcccgatggg gccgctggaa
    16801 ttaaccgatc tgattggtca ggacgtcaat tttgctgtca cctgttcggt gtttaacgct
    16861 ttctggcagg agcgtcgttt tttaccttcg ctggtgcaac aggaactggt gattggtgga
    16921 cggttgggca agaaaagtgg gctgggcgtg tacgactggc gcgcggaacg tgaggcagtt
    16981 gttggcctgg aagcggtaag cgacagtttt agcccaatga aagtagaaaa gaaaagtgac
    17041 ggcgtcacgg aaattgacga tgttttattg attgagacac aaggcgagac ggcacaggcg
    17101 ctggcaatac gactggcacg cccggtggta gtggtcgaca aaatggcggg caaggtggtg
    17161 accattgctg cggcagcggt gaacccggac tcagcgaccc gcaaggccat ttattacctg
    17221 caacagcagg gcaaaacagt gctgcaaatt gcagattacc caggaatgct gatttggcga
    17281 acggtagcaa tgatcatcaa tgaagccctt gatgcgctgc aaaaaggcgt ggcctctgaa
    17341 caggatatcg ataccgccat gcgtcttggg gtgaattatc catatggccc gcttgcctgg
    17401 ggagcgcaac ttggctggca gcgaatatta aggctcttgg aaaatctaca acatcactat
    17461 ggcgaagaac gctatcgccc atgttcattg ctgcgccaac gagcgcttct ggagagcggt
    17521 tatgagtcat aaggcctggc aaaatgccca tgcaatgtat gagaacgatg cctgcgccaa
    17581 agcgcttggc atcgacatta tctcaatgga tgaaggcttt gctgtagtga ccatgaccgt
    17641 cactgcacaa atgcttaacg gtcatcaaag ttgccacggc gggcagctat tttcactggc
    17701 tgatactgcc tttgcctacg cctgcaatag ccaggggctg gcagccgtcg cttctgcctg
    17761 cacgattgat tttttgcgtc caggctttgc cggagacacc ttaactgcta ctgcgcaggt
    17821 acgtcatcag ggcaagcaaa ccggtgttta cgacatcgaa attgttaacc aacaacaaaa
    17881 aacggttgcg ctgtttcgcg gtaaatctca ccgcatcggc ggcaccatta caggagaagc
    17941 ctgatgcgtg aagcctttat ctgtgacgga attcgtacgc caattggccg ctatggtggg
    18001 gcattatcag gtgttcgggc tgacgatctg gctgctatcc ctttgcggga gctgctggtg
    18061 cgaaacccgc gtctcgatgc ggagtgtatc gatgatgtga tcctcggctg tgctaatcag
    18121 gcgggagaag acaaccgtaa cgtagcccgg atggcgacgt tactggcggg gctgccgcag
    18181 agtgtttccg gcacaaccat taaccgcttg tgtggttccg ggctggacgc actggggttc
    18241 gccgcacggg cgattaaagc gggcgatggc gatttgctga tcgctggtgg cgtggagtca
    18301 atgtctcggg caccgtttgt tatgggcaag gcaaccagtg cattttctcg tcaggctgag
    18361 atgtttgata ccactattgg ctggcgattt gtgaacccgc tcatggctca acaatttgga
    18421 actgacagca tgccggaaac ggcagagaat gtagctgaac tgttaaaaat atcgcgagaa
    18481 gatcaagata gttttgcgct acgcagtcag caacgtacgg caaaagcgca atcctcaggc
    18541 attctggctg aggagattgt tccggttgtg ttgaaaaaca agaaaggcgt tgtaacagaa
    18601 atacaacatg atgagcatct gcgcccggaa acgacgctgg aacagttacg tgggttaaaa
    18661 gcaccatttc gtgccaatgg ggtgattacc gcaggcaatg cctccggggt gaatgacgga
    18721 gccgctgcgt tgattattgc cagtgaacag atggcagcag cgcaaggact gacaccgcgg
    18781 gcgcgaatcg tagccatggc aaccgccggg gtggaaccgc gcctgatggg gcttggtccg
    18841 gtgcctgcaa ctcgccgggt gctggaacgc gcggggctga gtattcacga tatggacgtg
    18901 attgaactga acgaagcgtt cgcggcccag gcgttgggtg tactacgcga attggggctg
    18961 cctgatgatg ccccacatgt taaccccaac ggaggcgcta tcgccttagg ccatccgttg
    19021 ggaatgagtg gtgcccgcct ggcactggct gccagccatg agctgcatcg gcgtaacggt
    19081 cgttacgcat tgtgcaccat gtgcatcggt gtcggtcagg gcatcgccat gattctggag
    19141 cgtgtttgag catatcaacc tgcgagtacc ctacaatgat aaccaataca aagcttgacc
    19201 cgattgaaac cgcgtctgtt gatgagttac aggcgttgca aacacaacgt ctgaaatgga
    19261 cgctcaaaca cgcgtatgaa aatgtcccga tgtatcggcg caaatttgac gcagcaggcg
    19321 tacatcctga tgatttcagg gaactttcag atctgcgtaa atttccctgt accaccaaac
    19381 aggatctgcg cgacaactat cccttcgaca cctttgccgt gccgatggaa caagtggtgc
    19441 gcattcatgc ttcttcggga accacaggta aaccgacagt tgtcggctat acgcaaaacg
    19501 atattgataa ctgggccaat attgtagcgc gttccttgcg tgcggcaggt ggcacgccca
    19561 aagacaaaat tcatgttgcc tatggttacg gcctgtttac tggtgggctg ggcgcgcact
    19621 atggtgccga acgtctgggc gctacggtga tcccgatgtc tggtgggcag acggaaaaac
    19681 aatcgcaact gatccgcgat tttcaaccag atatgatcat ggttacgcca tcttattgcc
    19741 ttaacctgat tgaagagctg gagcggcagt tgggcggtga tgccagcggt tgctcgctgc
    19801 gggttggagt gtttggtgct gagccgtgga cacaggccat gcgtaaagag attgaacggc
    19861 gtctggggat caccgcactg gatatttatg gcctgtcaga agtgatgggg ccgggggtgg
    19921 cgatggagtg tctggaaact accgacggcc caaccatttg ggaagatcat ttctatcctg
    19981 aaattgttaa tcctcatgac ggcacaccgc ttgccgatgg tgaacatggc gaactgttat
    20041 tcaccacgct gaccaaagaa gcattgccgg tcattcgtta ccgcacgcgt gatttaaccc
    20101 gactgttacc aggaacggcg cggactatgc gccgcatgga tcgcatcagc ggacgcagcg
    20161 atgacatgtt gatcattcgc ggtgtaaatg tcttcccgtc acaactggaa gaagagattg
    20221 tcaaattcga acatttatcg ccccattacc aactggaggt gaaccgccgg gggcatcttg
    20281 attcactttc tgtgaaagtg gagttgaaag aaagcagcct gacattgacg catgagcagc
    20341 gttgccaggt gtgccaccag ctgcgccatc ggattaagtc gatggtgggg atctctaccg
    20401 atgtgatgat cgttaactgt ggcagtatcc cgcgttcaga aggcaaggcg tgtcgggtgt
    20461 ttgatctgcg caatattgtt ggtgcctgac gtgtcgtctg gccctggtga ggtaaagcgc
    20521 cagggccaga agtcgatacg acctgtgcta tgattcataa atcacaacaa taacaacaga
    20581 ctgaagcgaa tgagtaaact tgatactttt atccaacatg ctgtaaacgc tgttccggtc
    20641 agtggcacat ctttgatctc ctctctgtat ggtgattcgc tttcccatcg tggtggtgaa
    20701 atctggcttg gtagtctggc tgctttgctg gaagggctgg gatttggtga gcgtttcgtg
    20761 cgcaccgctt tgtttcgtct taataaagaa ggctggctgg atgtttcccg catcgggcga
    20821 cgcagtttct atagcctcag tgataaaggc ttgcgcctga cgcgacgggc agaaagtaaa
    20881 atttatcgcg cagagcaacc tgcatgggat ggtaaatggc tcctgttgct ctcggaaggt
    20941 ttagataaat caacgctggc tgatgtcaaa aagcagttga tctggcaagg ttttggcgca
    21001 ctggcaccca gcctgatggc atcgccgtcg caaaaactgg cggatgtaca gacacttttg
    21061 catgaagcgg gggtggcgga taacgtgatt tgttttgaag cgcaaatacc actggcgctt
    21121 tctcgtgcag cactgcgtgc cagagtggaa gagtgctggc atttaactga acaaaatgcc
    21181 atgtacgaaa cctttattca gtcattccgc ccgctggtgc cgctgttaaa agaggcagca
    21241 gacgagttaa ccccggagcg tgcatttcat attcagcttt tactgatcca tttttatcgc
    21301 cgtgtcgtcc ttaaagaccc attgttgcca gaggagttgc ttccggcgca ctgggcaggg
    21361 catacggcgc gtcagctgtg tatcaacatt tatcagcgcg tagcgcctgc tgctttagcg
    21421 ttcgttagtg aaaaaggtga aacctcggtc ggtgaactgc ctgcgccggg aagcctgtat
    21481 tttcaacgtt ttggcggttt gaatattgaa caggaggcga tatgccaatt taccagatag
    21541 atggtctgac tccggttgtg ccagaagaga gttttgtcca tccgacagcg gtattgatcg
    21601 gcgatgttat tctaggcaag ggcgtttacg ttgggccaaa tgccagcctg cgtggcgatt
    21661 ttggtcgtat cgtggtgaaa gatggcgcga acattcagga taattgcgtt atgcacggtt
    21721 ttcccgagca ggatactgtc gtggaagaag atggacatat tggtcatagc gctatccttc
    21781 atggttgcat tatccgccgc aatgcattag tgggaatgaa cgcggtagtg atggacggcg
    21841 cggtgattgg cgagaacagc attgttggcg catccgcatt tgtgaaagcc aaagcagaaa
    21901 tgcctgccaa ttacctgatt gtcggcagcc cggcgaaagc gattcgtgaa ctcagtgagc
    21961 aggagctggc atggaaaaag cagggtacgc atgagtatca ggtgctggtg actcgttgta
    22021 agcagacgtt gcatcaagtt gagccattgc gggaagttga accgaacagg aaacgtctgg
    22081 tatttgatga gaatctgcga ccgaaacagt aacgtccaga tactgaacat tctaattttt
    22141 agcatgtgat acctaaaccg caacgctgat acaggttgcg gtttttttat tgccggatgc
    22201 ggcgctttac gcgttttgtt ttgtgttttt caggacaata gagcaactca tccagtaatc
    22261 ttgtttacgc cctcgcatta tttatctctt ttcgtttcta tactgatttt tcttaatccg
    22321 ttttattaca gggcagggtg cgatgagcag caatacattt actctcggta caaaatccgt
    22381 taaccgtctt ggttatggcg cgatgcaact ggcaggtcct ggggtttttg gtcccccaag
    22441 agatcgccac gtcgctataa ctgtgctgcg cgaggcgctg gcattgggcg tcaatcacat
    22501 tgataccagc gacttttatg gtccgcacgt caccaatcag attatccgcg aagcgcttta
    22561 tccttactct gacgacctga caattgtcac taaaattggt gcgcggcgtg gagaggacgc
    22621 ttcctggttg cccgcctttt ctccggcaga gttgcaaaaa gcggtgcacg ataatctacg
    22681 taatctcggc ctggacgtac tggatgtggt taacctgcgc gttatgatgg gagatggtca
    22741 tggcccagcg gaaggatcga ttgaggccag cctgaccgtg ctggcagaga tgcaacaaca
    22801 aggcctggta aaacatattg gcctgagcaa cgtcacaccg acgcaggttg cagaggcgcg
    22861 caagattgcc gaaattgtct gtgtgcaaaa cgaatacaac atcgcgcacc gtgctgatga
    22921 tgcaatgatt gatgctttgg cccacgatgg cattgcctac gtgccgttct tcccgctcgg
    22981 gggctttaca ccgctgcaat cctctacgct gtcggatgtt gctgcgagcc tgggtgcaac
    23041 acccatgcag gtggcgctgg cgtggctgtt acaacgttca ccgaatattt tgctgatccc
    23101 agggacgtct tctgttgcgc atttacggga gaatatggct gctgaaaaat tgcatctttc
    23161 tgaggaagtg ttgtctacgt tggatggtat ttcgcgagaa taacgaatat acaaaaggga
    23221 aagatgcatt tcccttttgt ttctttttta atggcatgga gtgcatatgt ttaagcccag
    23281 gatttgcagc tggataggtt tacttcccct gtttatgctg agtcttccag tacaggcgga
    23341 acttcgatgt gttgcaaatg cagttgatat tgaaccattt ttttctgcag ctaccgctga
    23401 agataaacaa caagttgaac aagctatcaa cagtagcgtg aatcttgtcc ccttcggttt
    23461 atctgcatcg gactggaaag tgcatcgtgg cgatttagtg gtagaaggta atatagagag
    23521 taatcaaaaa ttgattgttc ttggcaattt gacagtcaaa ggtaatattt ccactttctc
    23581 tctttctaat ccatgggtta ttctcggtaa cgtgacggca acgaatatag ttacggattc
    23641 accactgtta attaccggtt cgataaatgc aagtggactg gtatttatcg actcatatta
    23701 cgataatccg tctacgatta aggggggtat taatgcgcgt gggatattta tcaatgacat
    23761 aattgcacct gttgttgcgt cttcgacaaa tagtgaattc atggtccgtg cgagtgacaa
    23821 aaatgacact gaaaatgtca aaaaagcgct gatgataata aatcctgatg catattattg
    23881 gggtctaata aatgatgaag atgctctgaa agaaattttt aagcgaagca atattcgcat
    23941 ggcagggaat gtctgtaatc agatgaaaaa agaagcgctg tttcgcccta agccttctcc
    24001 tgagttagtg caggaattgc aaatgctgga tgaaggcaac gttgctgcat ttgaaggacg
    24061 agatattgcg acatttgatc ttgccataat gcgaactctc ccaaggctta aaggaatttc
    24121 agctaaccta cgcaaacaac ttattaatag caatgatgaa caaacgattg aaagtatggc
    24181 gagatatatg cctgacaatg agattctgga gttgaccgat cagcaattgg gttaccagcc
    24241 tgttgttctg gggttgctcg accgtgaacc gctctctgtc gaaataatga cgcgaatgag
    24301 tcgtctacct gatggtgtcg gtccgttgaa tcttgcgcta cgtgaaaatc tccctctgga
    24361 tatcgtaatg actctggcga aaagagattg ggatatgatc attcaggagc tttataaaga
    24421 tgcgtggtta ttgcctgaat ccattattga tggctatatc cgcagtgatg attcctctat
    24481 tcgtcaggtc ggtgctggag gacaactcac ctacaatcag gcaatgcagt tggcgaacga
    24541 ttcatcaaac aatgttgtca caagcttaac gttcaagctg gcagagatga aacaccatgg
    24601 tcaattattg cggatgacgc cacaagagag tgacaaagtt gcagcctatt tataccaaaa
    24661 attcgagaat gacgatgatc taatacgtgt gttattttta gcattgccag ataacttaca
    24721 gtttaatttt gttaaaagga tggagaaaaa atcccctgcc tacttttgct gtcgggatat
    24781 gcaggtaatt cactctgacg ctgctttaca acggttattg acacgtttca acgatcctga
    24841 ggggtggagt aatctggcga aaaatcagta tctgagcacg tcgatgaaac aaaaaatttg
    24901 gcaacgtgct ttatcgcatc ggaaaaataa tccgaaagca gattcagctg cgtatgaaac
    24961 cagcgctgat atgattttgt ctgagcttat cagccacggt gaagttgatg atcagatgct
    25021 attaaatgcc accgccctga tacgtttgga agactgggac tttttagaga gtgcattagt
    25081 tagttgggat aatttacccg ctgttgtcct caaggaatta cagcaaaata cgccacgcaa
    25141 tgatatttgg gcgaagtttt ttctgaggca ggaaaacagc tcccgtgcgc aggttgatga
    25201 agcattacgc gtctattatg cactcgaccc cgatgcgtta gcacaactgg atgtactggc
    25261 aaaacaaccg gatcgtatat ggtggagtac actggcgaaa agcaatctca catttttcaa
    25321 gttcggcgca cttaacaacc gccacacacc gcctgcagta ctggcggcag aaattgatcc
    25381 cgagtggtgg attgtggcga tgaataatcc ccgttttcca gttgatgtat taaaggcgag
    25441 gctgaaacgt gatcctttgc tggcgttaga gcttgttaac cctgaactgg atttagtccg
    25501 ccagctggcg cttaacggta agacgcgcgc aatacgggaa caagcgatga gaaaacttga
    25561 tgagttgtat tgatccacaa cgtaatattt cttaaccctt tgattaattg gggataatta
    25621 ttcctgacag tgagtcccca ataccttgat atattctgaa tttttaatga agcggcgtgt
    25681 tgcgatatct ccgtcagggg aattgatgca ccatagcgca aaccgaatta tcaaggattg
    25741 ataatgacgc tctaccagat aaaaccgctc tttcagtcgc tgttaaggcc gacgatgttt
    25801 tggctttaca agcaccacgt tacagcgaat cacatcactc ttgctgcact ggcgctttct
    25861 cttcttaccg gattgctgtt gatgttggtg gcacaaccca tcctctttat gctattgccc
    25921 atcgtgcttt ttatccgtat ggcgctcaat gcgctggatg gcatgttggc gcgtgagtgc
    25981 aatcagcaaa cacggtttgg ggcaattttg aatgagactg gtgatgttat ttccgatatt
    26041 gcgctctatt taccattttt atttttaccg gaaagtaacg catcactcgt gatactcatg
    26101 ttattttgca ccatattgac cgagttttgc ggtttactcg cgcagacgat taacggtgtt
    26161 cgcagttatg ccggtccatt tggcaaaagc gatcgcgcgt taatatttgg tctgtggggt
    26221 ctggccgttg ccatttatcc acagtggatg cagtggaata atcttttatg gagcattgct
    26281 tcaattctgc ttctctggac tgcgattaat cgttgtcgga gtgtgcttct tatgagcgct
    26341 gaaagataat gctggaaaaa tctctggcaa cacttttcgc gttgttaatt ttagcaacgc
    26401 tgataaacca ttttctgcta tggcggttac cggagagaaa agaggatgaa gttacattac
    26461 gtattcgtac ctggtggggc atcgtcattt gtttttcact ggtgatttca ggtccacgct
    26521 ggatgacgtt gacgtttttt gcgctgataa gttttctggc attgaaagaa tattgtacgc
    26581 ttatatctgt acattttccg cggtggttat attgggttat tcctcttaac tatttgttta
    26641 tcggttttaa ttgctttgag ctgtatctat tatttatacc tttggctggg tttctaatat
    26701 tagccaccgg gcaagttttt atgggggacc cctccggttt tctgcatacc gtgagcgcca
    26761 ttttttgggg ctggataatg accgttttcg ccttgagtca tgccgcctgg ttattaatgt
    26821 tgccaaccac aaatatccag ggcggggcgt tactggtgtt atttcttttg gcattaaccg
    26881 agtcaaacga tattgcacag tatttatggg ggaaatcctg cggcagaaga aaagtggtcc
    26941 ccaaagtcag cccgggaaaa acgctggaag gtctggtggg gggcgtaatc accaccatga
    27001 ttgcgtcact gattattgga ccgttgctga caccgctaaa tacattacag gcattattag
    27061 cgggtttgtt aattggtatt agtggttttt gcggcgatgt cgtgatgtca gccatcaaac
    27121 gagatattgg tgttaaagat agtgggaaac tattaccagg acatggcgga ctccttgaca
    27181 ggattgactc attaattttc accgctccgg tattttttta ttttattcgc tactgctgtt
    27241 actgaaggaa aatagagaaa atggaaaatt cacgcatcca tggggaacat ttttttacca
    27301 gcagtgataa tacagcgttg ttttatcggc actggcccgc tttacagccc ggggctaaaa
    27361 aggtcatcgt cttatttcat cgcggtcatg aacattctgg tcgtctacaa catctcgttg
    27421 atgaactggc gatgccagat actgtttttt atgcctggga tgcccgaggg catggacaaa
    27481 cttcggggcc gcgtggttat agcccatctc ttgcgcgttc agtgcaggat gtcgatgaat
    27541 ttgttcgttt tgctgccagc gatagccagg tcggactgga tgaggtggta gtgatcgcgc
    27601 aaagcgtcgg cgcagtgctg gttgccacat gggttcatga ttatgcgcct gcaattcgcg
    27661 ggctggtgct ggcttctccg gcctttaagg ttaaattgta tgtaccgctg gcacgtcctg
    27721 cgctggcgtt atggcatcgt ctgcgtggtc tgttttttat taattcctat gtgaaaggac
    27781 gctatttgac ccacgatcgg caacgggtgg cgagtttcaa taatgatccg ctgatcacac
    27841 gggcgattgc cgttaatatc ttgctcgatc tctacaaaac gtctgaacgt attattagag
    27901 atgcggcggc gattacgctc cccacgcaac ttctgatatc aggcgatgac tatgtggtgc
    27961 atcgccaacc gcagattgat ttttatcaga gattacgtag ccctctgaaa gagctgcatc
    28021 tgctgccagg cttttatcac gacacgttgg gtgaagagaa cagggcgcag gcatttgaaa
    28081 aaatgcaaag ctttattagt cgtttatatg ctaacaagtc acaaaaattt gattatcagc
    28141 atgaagaccg cactggacca tcagcggatc gctggcggct cctttcaggt ggacccgtgc
    28201 cattatcgcc ggttgatttg gcgtatcgct ttatgcgaaa ggcgatgaaa ttgttcgggg
    28261 cgcactctgc gggcctgcat ctcggaatga gcaccggctt tgattcaggc agttccctgg
    28321 attatgtcta tcaaaatcaa ccgcaaggca gtaacgcatt cgggcgctta attgacaaaa
    28381 tctacctgaa cagtgttggc tggcgcggta ttcgccagcg taaaacccat ttacaaatac
    28441 tgattaaaca agccgttgcc gatctccacg ccaaaggttt agccgtccgc gtggttgaca
    28501 ttgccgcagg gcatgggcgc tatgtactgg atgcactggc aaacgagcct gccgtaagcg
    28561 atattttgtt acgtgattac agtgagttaa atgttgcaca ggggcaagcg atgattgccc
    28621 aacggggaat gtctgggcgg gtgcgttttg aacagggcga tgcgtttaac ccggaggaac
    28681 tcagcgcgtt aactccgcgg cctacgctgg cgattgtctc tggcctgtat gagctttttc
    28741 ccgaaaatga gcaggtaaaa aactcactcg caggtcttgc caatgccatc gaaccgggcg
    28801 gcattctcat ctacaccggg cagccgtggc accctcaact ggagatgatt gccggggtgt
    28861 taaccagtca taaagatggt aaaccgtggg taatgcgcgt gcgttcgcaa ggggagatgg
    28921 attcactcgt gcgtgatgcc ggatttgata aatgcacaca acggattgat gagtggggta
    28981 tttttacggt ttcgatggcg gtgcgtcgtg ataactgaac gtcggaacgt attgctacaa
    29041 ggcgctggct ggttattgtt gctggccccg tttttcttct tcacctatgg atctcttaat
    29101 cagttcaccg cggttcagga ccttaacagc catgatatcc ccagtcaggt attcggttgg
    29161 gaaacggcga tcccttttct tccctggact attgttcctt actggagtct ggatctttta
    29221 tatggatttt cgctgttcgt ttgtagcacg acattcgaac agcgccgact tgtccaccgg
    29281 cttattctgg caacggtaat ggcctgctgc ggttttttgc tctatccgct gaagtttagt
    29341 tttatccgtc ctgaagtgag tggggtgacg ggatggctat tttcgcaact tgaactgttt
    29401 gatctgcctt ataaccagtc tccttcgctg catattattc tctgctggct actttggcgt
    29461 cactttcgtc agcatctggc tgtgaggtgg cgtaaagtct gcggcggatg gtttttactc
    29521 atcgccattt cgacgctgac gacatggcag catcatttta ttgatgtcat cacagggctg
    29581 gcggtaggta tgttgattga ctggatggtg cccgtcgacc gtcgttggaa ttatcagaaa
    29641 cctgatcaac gtcgaatcaa aatagcactg ccatatgtcg taggcgcggg ctcgtgcatt
    29701 gtgttgatgg agctaatgat gatggttcag ttatggtggt cagtctggtt atgttggcca
    29761 gtattatcgc tactcattat tggccggggg tacggtgggc ttggcgcgat aacaacaggg
    29821 aaagatagtc aggggaaact cccgcccgcc gtttactggc tgacattgcc ctggcgcatt
    29881 gggatgtggc tatctatgcg ttggttttgt cgtcgcctgg agccggtgag caaaatgact
    29941 gctggtgttt atttaggggc gtttccacga catattccgg cacagaatgc ggttctggac
    30001 gtcacctttg aattccctcg cggacgagcg acaaaagatc gactctattt ctgtgtaccg
    30061 atgctggatc tggtggttcc ggaagagggg gagctccgac aggccgtggc gatgctggaa
    30121 acattacgcg aagagcaagg cagcgttctg gtccattgcg cgttgggatt atcgcgcagt
    30181 gcgctggtag tggcggcatg gttgttatgt tacggacact gtaaaaccgt tgatgaagcg
    30241 attagttata ttcgagccag acgttcgcat attgtgctta aggaagatca caaagcgatg
    30301 ctgaaattat gggaaaacag gtaagtggat tgagatgtgg actgaactac agtccacatc
    30361 aagatcgtgt ccggttatgc agcaacaatg ctgtcgatgg ccgctttcgc gtcagactgc
    30421 gctttcgctg ccatttccgg accgtatgcg atcccttcgg cgaagacaaa tttcacatcg
    30481 gtaatgccga taaagccgag gaacgtggac agatacggcg tcaccaggtc cgttggtcca
    30541 tctttgtgga tcccaccgcg gctggtaata acgatggctt ttttacccgt taccagacct
    30601 tccggaccgt tctcggtata gcggaaagta acgcctgcgc gtgcaaccag gtcaaaatag
    30661 tttttcaact gagttgagat gttgaagtta tacatcggtg ccgcaataac gataacgtcg
    30721 tgggctttca gctcggcaat cagctcatcg gaaagcgcca gagcttcctg ctgacgcgga
    30781 gtcagcggcg catcgctcgg acgcagtgca ccaaccagtt cgccatccag taccggaatc
    30841 ggatttgcag ccaggtcgcg aacggtgatt tcatcagcgg agtgcttttc gcgccattgt
    30901 tcaacaaaat aatcggacaa ctgattagac tgagagtacc ctgccaggat gctggattta
    30961 agaactaata ccttgctcat ggtgtttcct tatagatgtt tgaatgggcg atgccccgtt
    31021 gcttgctgac actttattca caatcctgcc acagagatag cgcaataaat cgaagcctat
    31081 gttcgaattt attgaacaac gcatagaaag ccgcgatgtg gtactctata tctatcattt
    31141 aaaagaaaat taatcaggca gactactgcc cactaacgtt atgacagaac aacaaaaatt
    31201 gacctttacg gccttgcagc agcggctgga ttcgctgatg ctgcgcgaca gactgcgttt
    31261 ttctcggcgt ctgcacggtg ttaagaaggt taaaaatcct gatgcacaac aggccatttt
    31321 ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa gtcctgctgc gtgaagcggc
    31381 acgaccggaa attacttatc ctgacaattt accggttagt cagaaaaaac aggacattct
    31441 cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg gaaacgggtt ctggtaaaac
    31501 gactcagtta ccgaaaatct gtatggagct ggggcgcggg attaaagggc tgatcggcca
    31561 tacccagccg cgtcgcctgg cggcaagaac ggtggcgaac cgtattgcgg aagagctgaa
    31621 aacggagccg ggcggttgca tcggttacaa agtgcgtttc agcgatcacg ttagtgataa
    31681 cacgatggtc aagctgatga ccgacggtat cctgctggcg gagatccagc aagaccgcct
    31741 gctgatgcag tacgacacca tcattattga cgaagcacac gaacgcagcc tgaatatcga
    31801 ttttctgctc ggctatttga aagagttgct gccgcggcgt cctgacctaa aaatcattat
    31861 cacttccgcg actatcgacc cggaacgctt ttcgcgccac tttaataatg cgccgattat
    31921 cgaagtctcc ggtcggacct atccggtgga agtgcgctat cgaccgattg ttgaagaagc
    31981 cgacgacacc gagcgcgatc agttgcaggc gatttttgac gccgtagacg aactgagtca
    32041 ggaaagccct ggcgacattc tgatctttat gagtggtgag cgggaaattc gcgataccgc
    32101 cgatgcgctg aacaagctaa atctgcgtca taccgaaatc ttgccgcttt atgcgcggct
    32161 ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc ggacggcgca ttgtgctggc
    32221 gaccaacgtc gcggaaacct cgctgaccgt accggggatt aaatacgtta tcgaccccgg
    32281 tacagcacgt atcagccgct acagctatcg caccaaagtg cagcgtttgc cgattgaacc
    32341 catttcgcag gcgtctgcca atcagcgtaa aggccgctgt ggtcgtgtgt ccgaagggat
    32401 ctgtattcgt ctctattccg aagacgattt cctctcgcgc ccggagttta ccgatccgga
    32461 gattctgcgt accaacctgg cctcggttat tttgcagatg accgcgctgg ggctgggcga
    32521 tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc aatatccagg atggcgtgcg
    32581 tctgctcgaa gaactgggcg cgatcaccac ggatgaacag gccagcgcct ataaactgac
    32641 ggcgctcggt cgccagctct ctcagttgcc tgtcgaccca cgtctggcgc gtatggtgct
    32701 ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt atcacgtccg cgctctccat
    32761 tcaggatccg cgcgagcgtc cgatggataa acagcaggca tcggacgaaa aacatcgtcg
    32821 cttccacgac aaagaatccg atttcctcgc gtttgtaaat ctgtggaatt atcttggcga
    32881 gcagcaaaag gcgctttctt ctaacgcctt ccgtcgactg tgtcgtaccg attatctcaa
    32941 ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag ttgcgccagg tggtgaaaga
    33001 acttggcatt ccggtcaaca gcgaaccggc ggaatatcgc gaaattcaca ttgcgttgct
    33061 gacgggtttg ctttcccata tcggcatgaa agatgccgat aaacaagaat ataccggcgc
    33121 acgtaacgcg cgtttctcca tcttccccgg ttctggttta ttcaaaaaac cgccgaaatg
    33181 ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg gggcgcattg ctgcgcgtat
    33241 cgacccggaa tgggtggagc ctgtcgctca gcatttgatt aaacgcacct acagcgaacc
    33301 gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa aaagtcaccg tttacgggtt
    33361 gccgattgtt gccgcgcgta aggtcaacta cagccagatc gatccggcgt tatgccgtga
    33421 actctttatt cgccatgcgc tggtggaagg tgactggcag acgcgtcacg cattcttccg
    33481 tgaaaacctg aaactgcggg ccgaagtgga agagctggaa cacaaatcac gtcgccgcga
    33541 tattctggtt gatgacgaaa cgttgtttga gttctacgac cagcgcatca gccacgatgt
    33601 gatctctgcg cgccacttcg atagctggtg gaaaaaagtc agccgcgaaa cgcctgattt
    33661 gctcaacttt gaaaagagca tgttgatcaa agaaggggcg gaaaaaatca gcaagctgga
    33721 ttacccgaac ttctggcatc agggcaatct caagctgcgt ttgagctatc agtttgagcc
    33781 cggcgcggat gctgacggtg tgactgtgca tatcccgctg ccgttactta accaggttga
    33841 ggagaatggg tttgaatggc agatccccgg tctgcgccgc gaactggtga ttgctctgat
    33901 taaatcgttg ccgaaaccgg tacgccgtaa ttttgtaccc gcgccaaact acgccgaagc
    33961 gtttttaggc cgcgtcacac cgctggagtt accgttgctt gacagccttg agcgcgagtt
    34021 acggcggatg accggcgtta ccgttgaccg cgaagactgg cactgggatc aggtgcccga
    34081 tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac aagaagctaa aagaagggcg
    34141 ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg caggaaacgc tgtctgcggt
    34201 ggcggatgac ggtatcgagc agagcggctt acatatctgg agttttggtc agctgccgga
    34261 aagctacgaa cagaagcgtg gcaactacaa agtgaaggcg tggccggcgc tggtggatga
    34321 acgcgacagc gtggcgatca aactgtttga taatccgctg gaacaaaagc aggcaatgtg
    34381 gaacggtctt cgccgtctac tgctactgaa tattccctcg ccaatcaagt atttacatga
    34441 aaagttaccg aacaaagcca agctgggtct gtactttaac ccgtatggca aagtgctgga
    34501 gctgatcgac gactgtatct cctgcggtgt ggatcaactg atcgacgcca atggtggccc
    34561 ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa gtccgcgctg aactgaacga
    34621 cacggtggtg gatattgcga agcaggtcga gcaaatcctc actgcggtgt tcaatattaa
    34681 caaacgtctg aaagggcggg tggatatgac catggcgctg gggctttctg acattaaagc
    34741 gcagatgggc gggttggtat atcgcggttt tgtcactggt aacggcttca aacggctggg
    34801 cgacacgctg cgttatttgc aggcgattga aaaacggctg gaaaaactgg cggttgatcc
    34861 acatcgcgac cgtgcacaga tgctgaaagt cgaaaacgtc cagcaggcgt ggcagcaatg
    34921 gatcaacaaa ctgccgcccg cacgtcgtga ggatgaagat gtgaaagaga tccgctggat
    34981 gatagaagag ctgcgcgtta gttacttcgc tcaacaactt ggtacaactt atccgatttc
    35041 agataagcgt attttgcagg cgatggagca gattagcggt taacactgct atttgcctga
    35101 taaagaaaaa acccggtaag catttagcgc cgggtttttt attaattcta aaacggtaag
    35161 ggtaaaaatt caggaattca gaaaaataca attctctgct gcaagatgaa taatgtttat
    35221 ctacagcatt tccttaaaag atatgtcagg cttgcggagt ggcggttaag gacatacgac
    35281 ttcctccttt cagagtgctc cgcttctcac tattatctca cgcagtattc ttaagggaac
    35341 gataaggagg aaccatgaac attaccccgt ttccgacgct ttcgccggca actatagatg
    35401 ccataaatgt tatcggacag tggctggcgc aggatgattt ctccggtgag gtgccgtatc
    35461 aggccgattg cgtgatcctt gcaggcaatg cggttatgcc gactatcgac gcggcatgta
    35521 agattgctcg cgatcagcaa attcctttac tgattagtgg tggtatcggt cactcgacaa
    35581 cttttttgta tagcgccatc gcacagcatc cgcactacaa cactatccgc accactggca
    35641 gagcagaagc gaccatcctg gcggatatcg ctcatcagtt ctggcacatt ccgcatgaaa
    35701 aaatctggat tgaagaccag tcaacaaact gcggtgaaaa cgcacgcttt agcatcgcgc
    35761 tattgaatca ggccgtagaa cgagttcata cggctatcgt tgttcaggac cccaccatgc
    35821 agcggcgcac gatggcgacg ttccgccgta tgactgggga caatcccgat gcaccacgct
    35881 ggttaagtta tcccggattc gttcctcagt taggaaataa tgcagacagt gtaatcttta
    35941 ttaatcagtt acaaggatta tggccggttg agcgttatct ctcactactc actggcgagc
    36001 tgccgcgttt acgcgatgat agcgatggct acggtccccg cgggcgagat tttatcgttc
    36061 acgttgattt tccggcagaa gtcatccatg catggcaaac gctgaaacat gatgcggtgc
    36121 tcatcgaggc gatggaaagt cgctcgttac gttaaaaatt gcccgtttgt gaaccacttg
    36181 tttgcaaacg ggcatgactc ctgactttta tttctgcctt ttattccttt tacacttgtt
    36241 tttatgaagc ccttcacaga attgtccttt cacgatgccg tctctctgat gattgatgtt
    36301 aattaacaat gcattcaccg aaaataaaca tataaatcac aggagtcgcc catgtcagta
    36361 cccgttcaac atcctatgta tatcgatgga cagtttgtta cctggcgtgg agacgcatgg
    36421 attgatgtgg taaaccctgc tacagaggct gtcatttccc gcatacccga tggtcaggcc
    36481 gaggatgccc gtaaggcaat cgatgcagca gaacgtgcac aaccagaatg ggaagcgttg
    36541 cctgctattg aacgcgccag ttggttgcgc aaaatctccg ccgggatccg cgaacgcgcc
    36601 agtgaaatca gtgcgctgat tgttgaagaa gggggcaaga tccagcagct ggctgaagtc
    36661 gaagtggctt ttactgccga ctatatcgat tacatggcgg agtgggcacg gcgttacgag
    36721 ggcgagatta ttcaaagcga tcgtccagga gaaaatattc ttttgtttaa acgtgcgctt
    36781 ggtgtgacta ccggcattct gccgtggaac ttcccgttct tcctcattgc ccgcaaaatg
    36841 gctcccgctc ttttgaccgg taataccatc gtcattaaac ctagtgaatt tacgccaacc
    36901 aatgcgattg cattcgccaa aatcgtcgat gaaataggcc ttccgcgcgg cgtgtttaac
    36961 cttgtactgg ggcgtggtga aaccgttggg caagaactgg cgggtaaccc aaaggtcgca
    37021 atggtcagta tgacaggcag cgtctctgca ggtgagaaga tcatggcgac tgcggcgaaa
    37081 aacatcacca aagtgtgtct ggaattgggg ggtaaagcac cagctatcgt aatggacgat
    37141 gccgatcttg aactggcagt caaagccatc gttgattcac gcgtcattaa tagtgggcaa
    37201 gtgtgtaact gtgcagaacg tatttatgta cagaaaggca tttatgatca gttcgtcaat
    37261 cggctgggtg aagcgatgca ggcggttcaa tttggtaacc ccgctgaacg caacgacatt
    37321 gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa agtggcgcgc
    37381 gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg gaaaggatat
    37441 tattatccgc cgacattgct gctggatgtt cgccaggaaa tgtcgattat gcatgaggaa
    37501 acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc tatctcaatg
    37561 gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct gaacgtcgcg
    37621 atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga aaacttcgaa
    37681 gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc agatggtaaa
    37741 catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta atgagtaaaa
    37801 gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt ttcgagggtg
    37861 cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt tttcaccagt
    37921 tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga accgaaatgg
    37981 ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga ctcgttattg
    38041 gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt tccgagaatc
    38101 gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg acctttcagt
    38161 ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt agtgtgggga
    38221 atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg gccatccacc
    38281 agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc tatcccgaaa
    38341 ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga cgccacggaa
    38401 acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat agttttcatt
    38461 tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg cgcctgcgat
    38521 ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcccc tttcgctttc
    38581 cacggaatat ttttagcctc tttttcggcg taaaccgaga tacttttccc atcaacgata
    38641 agtgaatctt ccgtaaaatc aacgctccag gggaacggac catagtttga atcatgtttc
    38701 agcagatagg cgagaatttt tggggaagtg agatcattaa tagcgacaac gtctatgttg
    38761 cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa accgttaata
    38821 ccaactttac tcatggtttt ctcctgtcag gaacgttcgg atgaaaattg atcctttcca
    38881 ggcttagccc aggattgtgg gatgggcaat ctccattctt acagtgaaac gtaacgtact
    38941 gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt taacggaaaa
    39001 ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa tcagcattca
    39061 ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc gtggtttctt
    39121 cccacgtagc gatcggccac tcatcaacat ggttcatgtt tcctgtggta tctcaattct
    39181 cgtgctgatg gtggttcgtc ttttgttaag actgaaatac ccaaccccgc cgattatacc
    39241 taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga tttatctcct
    39301 gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca acccgtggtt
    39361 tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg ttgatagctt
    39421 aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt tgcacgctgc
    39481 ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca tgatgccgcg
    39541 taaacgttcc tgaaggatat ttaaagaaaa cgcctgtact aaaaccgacc cgtggtacag
    39601 gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg acaaataaaa
    39661 ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc cgatctttga
    39721 tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca taagattcga
    39781 taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga acgcttgttt
    39841 ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg atctcacact
    39901 gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcctcg aaaaaaacat
    39961 gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt cagatgcgcg
    40021 gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca gcaacctcct
    40081 tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt gtgaatgtca
    40141 gaatcgtaat gcagataatg agcagacaca ccaccagagg gttgtgcttc atagcctttc
    40201 tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat tcgttggcct
    40261 cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta gcctgaaaac
    40321 agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga aaataatcac
    40381 ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc cagtttagcc
    40441 attcgttact ctcttcattc caatagcatt aattttctct gcaataattg ttgtaaaaat
    40501 gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg actttctatc
    40561 aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca tcgattaggt
    40621 tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg tatcttagtt
    40681 ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga tcgcgatcag
    40741 cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc agattttttg
    40801 cgttcagccc ggatcaatat gattcaggcc ggggctgcga gtcgtattgc ggaaatggaa
    40861 gcaatgaagc gcaatattgc gcaagccgaa tcggagatta aacagtcgca gcaaggttat
    40921 cgtgcttatc agaatcgacc ggtgaaaaca cctgctgatg aagccctcga cactgaatta
    40981 aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata tgccaaaaat
    41041 ggcatgtttg aagcgattat taatcatgaa agtgagcaga tccgaccgct ggataatgct
    41101 tataccgata ttttgaacaa agccgttaag atacgtagca ccagggccaa ccaactggcg
    41161 gaactggccc atcagcgcac ccgcctgggt gggatgttca tgattggcgc gtttgtgctt
    41221 gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat tcgtccactg
    41281 caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat gaaggatgaa
    41341 ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat gcagcattca
    41401 ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg tggcaccagc
    41461 gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc ggcggcaatc
    41521 gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa tgcggataac
    41581 gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga tggcgggcag
    41641 acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa gaaaatttct
    41701 gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc actgaatgct
    41761 gccgttgaag ccgcgcgagc gggtgagcaa ggccgtggat ttgccgttgt cgccagcgaa
    41821 gtacggacac tcgcaagccg cagcgcccaa gcggcgaaag agattgaagg cttgatcagt
    41881 gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggcagg gaaaaccatg
    41941 agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat cgccgccgcc
    42001 tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga aatggataag
    42061 gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt gtcccttgaa
    42121 gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa acattctgtg
    42181 tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg aaaatgttca
    42241 aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga atagcgttaa
    42301 tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt ccaatcggtt
    42361 cgactgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc aggtcgtctt
    42421 ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt tgacgagata
    42481 gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg ctctgcacta
    42541 atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc ggccattcca
    42601 gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg acaaccagct
    42661 tcaacgattc aagaaacagc agttcgtaat taagcccggt catcagttca ggatctgaca
    42721 tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc atagggttac
    42781 tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga cctataaccg
    42841 aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca tcattattaa
    42901 gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga actctgactg
    42961 catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga ccacgctcaa
    43021 acaagcgagc gccagtcagc tgctccagtt cattcaatgt cttagagagc gcaggttgac
    43081 tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca gctacgaatg
    43141 tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc ataagcgatg
    43201 ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa cttgattgca
    43261 aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat tgatgttttc
    43321 gcggcgagat cacagtttgt aaattcttcc cgcaagagtg aatgcggtta cctacactcc
    43381 agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg atgagattcg
    43441 ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc aatatggcac
    43501 gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc ctcaactgca
    43561 cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc atggggcgat
    43621 tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca ttatggggat
    43681 gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt cgacagcatt
    43741 tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta cctccttact
    43801 ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga ttctgcgtca
    43861 gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc agcagggcgg
    43921 ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt ttcgctggca
    43981 ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac atcggttgat
    44041 tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac gtacgctgga
    44101 tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa ttctcatcga
    44161 ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta aagcactgga
    44221 agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct ttggtgaaat
    44281 tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg atcttctgcg
    44341 taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg aaaccttccg
    44401 cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt tccggtaccg
    44461 tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg atccacagcc
    44521 cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt tcttgcccgt
    44581 tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac gcagtcacgg
    44641 taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg atgagttcca
    44701 gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt aaattctgac
    44761 catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat cccatcgaca
    44821 taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta ttaataaata
    44881 ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg accagcgcgg
    44941 gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg atttattaaa
    45001 ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct tttttctcag
    45061 gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt tgacttctcc
    45121 attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc gcctcgtccg
    45181 ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca atacgacgcc
    45241 gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt cttccatatg
    45301 ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac acatctggcg
    45361 cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcgggtgt tgataccaaa
    45421 caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa agcccccgaa
    45481 ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc cgttgatgat
    45541 acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac cgacagtaaa
    45601 gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg ggcaactacc
    45661 tttaccgttt atgcgttgct cgatagcgcc agcattactg gtgcctataa gttcactatc
    45721 cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc gcgcaaagac
    45781 attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac taatgaacgt
    45841 cggatgtgcg atacaattca tccgcaaatt catgactctg atcgtctgtc catgtggcgg
    45901 ggcaacggcg agtggatttg ccgtccgctg aataatccgc aaaaattgca gttcaatgct
    45961 tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga cttctcccat
    46021 tatcaggaca ttatgggctg gtataacaaa cgcccaagtc tgtgggtgga accgcgtaac
    46081 aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga aacgctggat
    46141 aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga gtttgcattc
    46201 cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc gcgcgttatg
    46261 gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga acactatccc
    46321 gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa agctgccgcg
    46381 ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca aatcgaaatt
    46441 ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc gacttcggac
    46501 tccactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc tatcagtgaa
    46561 acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt tgacgaccgc
    46621 gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc tggttaaaca
    46681 cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg tatttcagat
    46741 acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag atttaatatc
    46801 ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca ataaacttga
    46861 tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg aagtggtccg
    46921 catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc agcaggagag
    46981 cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta acaacagcat
    47041 ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact tgaattacat
    47101 gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa taaaacctgg
    47161 ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac gcgaaaaacg
    47221 gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat gattttcaaa
    47281 gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa taaaactgct
    47341 gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc tcaggcgctg
    47401 caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt gatcaaatgt
    47461 cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat ccttgaaggt
    47521 tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt atacgcgcgt
    47581 attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct cgccgttaat
    47641 gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga cggcgtggtc
    47701 ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct caccttgtat
    47761 tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc cgccaatcaa
    47821 gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca cctgcgaact
    47881 gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg cgtgaccgta
    47941 gactttagcg caatcgcata gccagacgtt gttctcttca ttaccttcaa cactggcaaa
    48001 atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat cgccataaat
    48061 ttgtgcctgg tggacaatac gcgaggcgct aaccccgcgc gatcataaat ttggagcagg
    48121 agttgatggt cgggcgttaa gccttgtgct gcgacgatca tagaatgttg attaattaag
    48181 gcgtgaccaa atattctaca ttgcccgcaa accagtgagt catgaatggt gacgctgtca
    48241 cttatatatg caccctggct gatttcacta ttatcgatcc agacattatc cgttgcgtaa
    48301 acctctcccc acagcacgct ggtgccggtg atgcgcgtat taccggaaat caccgctccg
    48361 ccgaacgcaa tagcgttctg gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt
    48421 tcgcgatcga tccagccgcc agcggttcct gctatcacat cgttaaaatc gctaatggcg
    48481 ataatctgcc gtaataacac atttttttta gtgccatctt cctgataact aaaggctcgc
    48541 tgttcttcac ttagacgata tttgcgcata gcgttttccc acaggtgact tactataacc
    48601 gtagcaaatt ctgcggctct ggctatgctc ga
//
LOCUS       DAPGWD010000017        48491 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPGWD010000017 DAPGWD010000000
VERSION     DAPGWD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.017"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            283..1500
                     /gene="alaA"
                     /locus_tag="RHX88_000923"
     CDS             283..1500
                     /gene="alaA"
                     /locus_tag="RHX88_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDV2229649.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1584..2183
                     /gene="yfbR"
                     /locus_tag="RHX88_000924"
     CDS             1584..2183
                     /gene="yfbR"
                     /locus_tag="RHX88_000924"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV2229650.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2242..4074)
                     /locus_tag="RHX88_000925"
     CDS             complement(2242..4074)
                     /locus_tag="RHX88_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDV2229651.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4161..4811)
                     /gene="hxpA"
                     /locus_tag="RHX88_000926"
     CDS             complement(4161..4811)
                     /gene="hxpA"
                     /locus_tag="RHX88_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDV2229652.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGPAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4822..5316)
                     /locus_tag="RHX88_000927"
     CDS             complement(4822..5316)
                     /locus_tag="RHX88_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDV2229653.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5399..5854)
                     /locus_tag="RHX88_000928"
     CDS             complement(5399..5854)
                     /locus_tag="RHX88_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDV2229654.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6192..7394
                     /gene="ackA"
                     /locus_tag="RHX88_000929"
     CDS             6192..7394
                     /gene="ackA"
                     /locus_tag="RHX88_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDV2229655.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7469..9613
                     /gene="pta"
                     /locus_tag="RHX88_000930"
     CDS             7469..9613
                     /gene="pta"
                     /locus_tag="RHX88_000930"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV2229656.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9803..11323
                     /gene="yfcC"
                     /locus_tag="RHX88_000931"
     CDS             9803..11323
                     /gene="yfcC"
                     /locus_tag="RHX88_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDV2229657.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11356..11898)
                     /gene="yfcD"
                     /locus_tag="RHX88_000932"
     CDS             complement(11356..11898)
                     /gene="yfcD"
                     /locus_tag="RHX88_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDV2229658.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11956..12507)
                     /gene="yfcE"
                     /locus_tag="RHX88_000933"
     CDS             complement(11956..12507)
                     /gene="yfcE"
                     /locus_tag="RHX88_000933"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDV2229659.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12563..13207)
                     /gene="yfcF"
                     /locus_tag="RHX88_000934"
     CDS             complement(12563..13207)
                     /gene="yfcF"
                     /locus_tag="RHX88_000934"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDV2229660.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13343..13990
                     /gene="yfcG"
                     /locus_tag="RHX88_000935"
     CDS             13343..13990
                     /gene="yfcG"
                     /locus_tag="RHX88_000935"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDV2229661.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14047..14409
                     /gene="folX"
                     /locus_tag="RHX88_000936"
     CDS             14047..14409
                     /gene="folX"
                     /locus_tag="RHX88_000936"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDV2229662.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14430..15323
                     /locus_tag="RHX88_000937"
     CDS             14430..15323
                     /locus_tag="RHX88_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDV2229663.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15371..16273)
                     /gene="rpnB"
                     /locus_tag="RHX88_000938"
     CDS             complement(15371..16273)
                     /gene="rpnB"
                     /locus_tag="RHX88_000938"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDV2229664.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGQNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16470..17243)
                     /gene="hisP"
                     /locus_tag="RHX88_000939"
     CDS             complement(16470..17243)
                     /gene="hisP"
                     /locus_tag="RHX88_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDV2229665.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17251..17967)
                     /locus_tag="RHX88_000940"
     CDS             complement(17251..17967)
                     /locus_tag="RHX88_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2229666.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17964..18650)
                     /gene="hisQ"
                     /locus_tag="RHX88_000941"
     CDS             complement(17964..18650)
                     /gene="hisQ"
                     /locus_tag="RHX88_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDV2229667.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18740..19522)
                     /gene="hisJ"
                     /locus_tag="RHX88_000942"
     CDS             complement(18740..19522)
                     /gene="hisJ"
                     /locus_tag="RHX88_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDV2229668.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19743..20525)
                     /gene="argT"
                     /locus_tag="RHX88_000943"
     CDS             complement(19743..20525)
                     /gene="argT"
                     /locus_tag="RHX88_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDV2229669.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20791..21360)
                     /gene="ubiX"
                     /locus_tag="RHX88_000944"
     CDS             complement(20791..21360)
                     /gene="ubiX"
                     /locus_tag="RHX88_000944"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDV2229670.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21455..22972)
                     /gene="purF"
                     /locus_tag="RHX88_000945"
     CDS             complement(21455..22972)
                     /gene="purF"
                     /locus_tag="RHX88_000945"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDV2229671.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23009..23497)
                     /gene="cvpA"
                     /locus_tag="RHX88_000946"
     CDS             complement(23009..23497)
                     /gene="cvpA"
                     /locus_tag="RHX88_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDV2229672.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23756..24418)
                     /gene="dedD"
                     /locus_tag="RHX88_000947"
     CDS             complement(23756..24418)
                     /gene="dedD"
                     /locus_tag="RHX88_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDV2229673.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24408..25676)
                     /gene="folC"
                     /locus_tag="RHX88_000948"
     CDS             complement(24408..25676)
                     /gene="folC"
                     /locus_tag="RHX88_000948"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDV2229674.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25746..26660)
                     /gene="accD"
                     /locus_tag="RHX88_000949"
     CDS             complement(25746..26660)
                     /gene="accD"
                     /locus_tag="RHX88_000949"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDV2229675.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26816..27475)
                     /locus_tag="RHX88_000950"
     CDS             complement(26816..27475)
                     /locus_tag="RHX88_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2229676.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27558..28370)
                     /gene="truA"
                     /locus_tag="RHX88_000951"
     CDS             complement(27558..28370)
                     /gene="truA"
                     /locus_tag="RHX88_000951"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDV2229677.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28370..29383)
                     /locus_tag="RHX88_000952"
     CDS             complement(28370..29383)
                     /locus_tag="RHX88_000952"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV2229678.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29449..30585)
                     /gene="pdxB"
                     /locus_tag="RHX88_000953"
     CDS             complement(29449..30585)
                     /gene="pdxB"
                     /locus_tag="RHX88_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDV2229679.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30684..31679
                     /gene="flk"
                     /locus_tag="RHX88_000954"
     CDS             30684..31679
                     /gene="flk"
                     /locus_tag="RHX88_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDV2229680.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31676..32854)
                     /locus_tag="RHX88_000955"
     CDS             complement(31676..32854)
                     /locus_tag="RHX88_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2229681.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33138..34358)
                     /gene="fabB"
                     /locus_tag="RHX88_000956"
     CDS             complement(33138..34358)
                     /gene="fabB"
                     /locus_tag="RHX88_000956"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDV2229682.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34517..36523
                     /gene="mnmC"
                     /locus_tag="RHX88_000957"
     CDS             34517..36523
                     /gene="mnmC"
                     /locus_tag="RHX88_000957"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDV2229683.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMAKLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36644..36922)
                     /locus_tag="RHX88_000958"
     CDS             complement(36644..36922)
                     /locus_tag="RHX88_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDV2229684.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36956..37504)
                     /gene="epmC"
                     /locus_tag="RHX88_000959"
     CDS             complement(36956..37504)
                     /gene="epmC"
                     /locus_tag="RHX88_000959"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDV2229685.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37504..38313)
                     /locus_tag="RHX88_000960"
     CDS             complement(37504..38313)
                     /locus_tag="RHX88_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV2229686.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38313..39137)
                     /gene="mepA"
                     /locus_tag="RHX88_000961"
     CDS             complement(38313..39137)
                     /gene="mepA"
                     /locus_tag="RHX88_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDV2229687.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39141..40226)
                     /gene="aroC"
                     /locus_tag="RHX88_000962"
     CDS             complement(39141..40226)
                     /gene="aroC"
                     /locus_tag="RHX88_000962"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDV2229688.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40261..41193)
                     /gene="prmB"
                     /locus_tag="RHX88_000963"
     CDS             complement(40261..41193)
                     /gene="prmB"
                     /locus_tag="RHX88_000963"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV2229689.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            41359..41910
                     /gene="smrB"
                     /locus_tag="RHX88_000964"
     CDS             41359..41910
                     /gene="smrB"
                     /locus_tag="RHX88_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDV2229690.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41983..42837)
                     /locus_tag="RHX88_000965"
     CDS             complement(41983..42837)
                     /locus_tag="RHX88_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDV2229691.1"
                     /translation="MKILRHVLCLLLLLMFNTCGQAATKVGELYVPAGASSPATEFEF
                     HLIIATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSRVSMSACPGLEQ
                     YDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDDDFPDYIGPTKV
                     SSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCNGNNFDE
                     RGAYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCR
                     FTYILNMY"
     gene            complement(42839..43378)
                     /locus_tag="RHX88_000966"
     CDS             complement(42839..43378)
                     /locus_tag="RHX88_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2229692.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGAQAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVSGQSAHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43375..43863)
                     /locus_tag="RHX88_000967"
     CDS             complement(43375..43863)
                     /locus_tag="RHX88_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2229693.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            complement(43860..44369)
                     /locus_tag="RHX88_000968"
     CDS             complement(43860..44369)
                     /locus_tag="RHX88_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2229694.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44385..45137)
                     /locus_tag="RHX88_000969"
     CDS             complement(44385..45137)
                     /locus_tag="RHX88_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2229695.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45157..47799)
                     /gene="yfcU"
                     /locus_tag="RHX88_000970"
     CDS             complement(45157..47799)
                     /gene="yfcU"
                     /locus_tag="RHX88_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HDV2229696.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47881..48444)
                     /locus_tag="RHX88_000971"
     CDS             complement(47881..48444)
                     /locus_tag="RHX88_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2229697.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11852 a        13136 c        12109 g        11394 t
ORIGIN      
        1 attagtgaat tcttaaatga ggcattttca cacaattatc ttacagataa aaaaaccaga
       61 cttacaatta agaatcagaa caattcacta tataacattg catgtaaagc atatacacct
      121 cattattttg tcattattaa gttattaaca gcacaatcga gccttcccct ctggcaaaat
      181 cttattctgc agaccttcaa aacaccgtcc tgggggagta cattgttcta agctgacttc
      241 cacggcaggg agtggcgata acagcaaaaa aggtcaagat tcatgtcccc cattgaaaaa
      301 tccagcaaat tagagaatgt ctgttatgac atccgtggtc cggtgctgaa agaagcaaaa
      361 cgcctggaag aagaaggtaa caaggtactg aaactgaaca tcggcaaccc agccccgttc
      421 ggttttgacg cgccagatga aatcctcgtt gacgtgatac gcaacctgcc taccgctcaa
      481 gggtattgcg attccaaagg tctttactcc gcgcgtaaag ccatcatgca gcactaccag
      541 gctcgtggca tgcgtgatgt taccgtggaa gatatttaca tcggcaatgg tgtatcggag
      601 cttatcgttc aggcaatgca ggcattgctg aacagcgggg acgaaatgtt ggttcctgca
      661 ccagattacc cactatggac cgcggcggtt tcgctttcca gcggtaaagc ggtgcattat
      721 ctttgcgatg aatcctctga ctggttcccg gacctcgatg atattcgcgc taaaattacg
      781 cctcgtacgc gtgggatcgt tataatcaac ccaaataacc caaccggcgc ggtatattcc
      841 aaagagcttt taatggagat tgtggagatt gcacgtcagc ataatctcat tatcttcgcc
      901 gatgaaattt atgacaaaat tctttacgac gacgctgagc atcactcaat tgcgccgctg
      961 gcacctgacc tgctgaccat tacctttaac ggactgtcga aaacgtaccg cgttgcaggc
     1021 ttccgtcagg ggtggatggt gttgaacggg ccgaaaaaac acgccaaagg ctacatcgaa
     1081 ggtctggaaa tgctggcttc aatgcgcctg tgtgctaacg ttcctgcgca acacgccatt
     1141 cagaccgcgc tgggtggtta tcagagcatc agtgaattta ttacccctgg cggtcgtctt
     1201 tatgagcagc gtaaccgcgc gtgggaactg atcaacgata ttccgggcgt ttcctgcgtg
     1261 aaacctcgtg gtgcgctgta tatgttcccg aaaatcgacg ccaaacgctt taacattcac
     1321 gacgatcaga aaatggtgct ggatttcctg ttgcaggaaa aagttctgtt ggtgcaaggg
     1381 acggcattca actggccgtg gccggatcac ttccgcattg tcacgctacc gcgtgtcgat
     1441 gatatcgagc tgtctttgag caagttcgcg cgtttccttt ctggttatca tcagctgtaa
     1501 tcttaatttc actgccggag attgcatccg gcagcgttat cccgccacaa tgacctgatg
     1561 atgtcatcat acgtaaggtc actatgaaac agagccattt ttttgcccat ctctcccgcc
     1621 tgaaactcat taaccgctgg ccgctcatgc gcaacgtgcg gacggaaaat gtgtccgaac
     1681 acagtttgca ggtagcgatg gtcgcccatg cgctggcagc tatcaaaaat cggaaatttg
     1741 gcggtaatgt caacgccgaa cgtatcgctt tactggcgat gtaccacgat gcctcagaag
     1801 tgctcaccgg cgatctccct actccggtga aatacttcaa ttcgcaaatc gctcaggagt
     1861 acaaggctat tgaaaaaatc gctcagcaaa aactggtcga tatggttccg gaagagctgc
     1921 gggatatctt tgcgccgtta attgacgagc atgcatatag cgatgaagaa aaatcgctgg
     1981 tgaaacaggc agatgcactg tgtgcatatc tgaaatgtct ggaagaactc gcggccggaa
     2041 ataatgaatt cttgctggca aaaacgcgac tggaagcgac gcttgaagcg cgtcgcagcc
     2101 aggagatgga ctacttcatg gaagtatttg ttcccagctt ccatctttcg ctcgatgaga
     2161 ttagccagga ttcaccgctg taagcagccg gagtctgcgt cgcatcaggc aataagcgcc
     2221 ggatgcgaca tcaggctctt gtcaaaacgg aaacagcatc gggatcatca ccacacaaac
     2281 cgccatcacg ataatggtga acggtacccc caacttcaca aagtcactga agctgtaatt
     2341 tcccggacct aaaaccagtg tgttaacagg tgaagaaacc ggtgtcataa aggcggcgga
     2401 tgctgccatc gccacgacca tcgcgaatgg ataaggcgac acccccatcg ttttggcagc
     2461 agccagcgca atcggagcca tcaacaccgc cgtcgcggta ttagagataa atagcccaat
     2521 aaccgccgac aagacaaaca aacaacccag catcatatgt ggcccgtaac caccgccaat
     2581 gtccatcagc cctttcaccg ccagcgcgac acctcccgtt ttctgtaatg ccacagcaaa
     2641 cggcatcatc ccaacgatca aaataatgct cggccagtga atggatttat aggcgctttc
     2701 agcatctata cagcggaatt tccccatcag caggcaggcg atgatagcgg caacaggatt
     2761 aggaatttca tctgtcagca ttaacgccac cattagcacc agacagaaaa tggcatgggg
     2821 tgcctggctg tgcgcgggcg atgcttcact cacctcttcc ggtaagttca gcgctacgaa
     2881 gtcgcggccc tgtttggcca gcataccgat cagtttccag ttacccacaa ccaggatgat
     2941 atcgcccagc agcagaggct catccgccag cgaaccttcc agcgccacgc cattgcgctt
     3001 tagccccacc acattcagtc cgtagcgggt acgaaaacca atttcgcgca ccgatttacc
     3061 aatcagttct gactcaggaa ttaatgaaat ctctgccatg cccacatcaa gggcctggtc
     3121 agaaaaatac tcgccgcgca gtaccatcgg ctccagcaat tgctcactac aaaattgccg
     3181 gagatcgaca tcagccgcag acatatcaat aagcaaaacg tcacgcgcgc gaaattcaga
     3241 aaccccatta acgttcacga taacgcgacg aaaacgccgc cagcgttcaa caccgatgac
     3301 gttagcgcca taacgctcac gtaatttgag atcatccagc cgttgaccta tcattggcga
     3361 tccggggcga atagccagac gtcgcgcacg cccggtcagt cgatattcac ggataagatc
     3421 gcgaaaggtt cgacgcgtcc agccttcgcg ctgcggggtc tgggtatccc ctttcagcat
     3481 gaaacgcatc actaacatat acaaaatacc cagcaccagc acgaccaggc caataggtgt
     3541 tacgctaaag aaactgaagc cgtgatagcc ttcacgcagc aattcactgt tgactaccag
     3601 gttcggcggc gtcgccacca gcgtcatcat gccgctaatc agcccggcaa aacttaacgg
     3661 catcatcaga cgtgacggcg aagtttgcat acgcatggca acgcttaaca ccacgggaat
     3721 aaagatagcg acaacgccgg ttgaactcat aaacgcgcca agccccgcga cggtcagcat
     3781 caacaaaacc aacattttga tttcactgtt gcccgccact ttgaccagcc atgttcccat
     3841 tacggtggca acaccggtac ggaccaaacc atcgccaata ataaacaagg cggcaatcag
     3901 gacaacgtta ggatcagaaa agccggaaaa tacttcgggg accgtcagcg ttccgcttaa
     3961 tgcaaacgcg acaataacaa acaaagcgac cgcatccata cgcactctgc ccgtcgcaaa
     4021 caagacgatg gcaacggcca gtaatgaaag aacccaaatc aattcaccgt tcacaactta
     4081 tccttgttaa ttgaggggga tgacttgatt ctgccataaa aaagccccga cgagacgggg
     4141 ctaaatcatg atcaggtgtt tcactgaata ataacatcgc catttggctg tttggtcaca
     4201 gtaatttgct ccagactgtg gaggaccaaa tcgacctcat tcaggcgcgg ggtatctgcc
     4261 ggagcgttaa ccgcaatgac atggcaaccc gccgccgggc cagaaagcac gccagcggga
     4321 gcatcttcca ccaccacaca ctcctgcggc gcaagcccca gcagctgcgc acctaacaga
     4381 tacgcatcag gttctggttt tccgcgcttc actcgctcag cggttacaaa cacctctggt
     4441 gcgggaagcc cagctatttt atggcgcgct cgcgctaccg gcatggagcc agaagtcaca
     4501 atggcccacg gaatacctgc tttattcaaa tgattgagta aggcgattgc ccccggaagc
     4561 gcggtaatac cttcggtttc cgtggcctcg atgtgctcca gacgcgtaaa ctcggcggca
     4621 atatcagcct cggatttgcc cgccataaaa tggcgcaaag aggtgatcgc ctgtttaccg
     4681 tgaatgaaag ccagcacctc ttccggcgct aacccatgac gtctggccca gttgctccac
     4741 gcccgttcta ccgcaggcag ggaatccacc agcgttccat caagatcaaa cagaaaacct
     4801 ttgcaccgca cgcgggcctc ctcaggcgtt gataatttga ttaatttcgt tggcgctcaa
     4861 atggtactga cgcgggcagg catgccacac attaagcatg cgctgatatt tttcccacat
     4921 tggcgtctgg gcgttaaaac cgtgagttcc ggcgtcaaaa tgggtatagc gcccttccac
     4981 attaaccata aagcggacat aaccgaggta acgtgcttca gtggcggcgt caaagccgag
     5041 gaaggtgaca cgacgttcat cgatggattg ctggtcctgc aaattagacc aggaaacatg
     5101 caacgcatga tacatctcca taatgtcgat gatagtgcgg caggtttctt ctttcagctc
     5161 gccaaactcg cgatccaatt cacgcatctg taatccgtaa ccacgctcaa taattgtttg
     5221 caggcgacgg taacgttcag catttgccgg atcgagcata gtcatcatct tgtactgatt
     5281 agacaaaata agacgttgcg cgttggtcat ttccattgtt gactcctgta tcactatact
     5341 acggtgaaaa aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt
     5401 acaaatcatc aaggaaagtt ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta
     5461 atgcctggta atcaggcttg ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt
     5521 taccgcgaac ttcataaaac cagttaagga ttgcaggggg taatggcgtt acagaacgct
     5581 tgcccagcca ccacaatccc tgcatgggta aacttaaggc gaacagcgca gtggcaactg
     5641 ccggcccaag ctgaccgccc agggcaatct gccagcagag agtaaatacg gcgatcggcg
     5701 gcataaaacg gatcgcataa cgcgtcatct tgataacgcg attttcgaca aagaccgggg
     5761 caaggcgttt ttccagcggc cacgtctttg agtaatgctg tccccggcga aacaagctaa
     5821 aaaaattaac agaacgatta tccggcgttg acatgcttca cctcaacttc acatataaag
     5881 attcaaaaat ttgtgcaaat tcacaactca gcgggacaac gttcaaaaca ttttgtcttc
     5941 catacccact atcaggtatc ctttagcagc ctgaaggcct aagtagtaca tattcattga
     6001 gtcgtcaaat tcatatacat tatgccattg gctgaaaatt acgcaaaatg gcatagactc
     6061 aagatatttc ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc
     6121 ggtgtcatca tgcgctacgc tctatggctc cctgacgttt ttttagccac gtatcaatta
     6181 taggtacttc catgtcgagt aagttagtac tggttctgaa ctgcggtagt tcttcactga
     6241 aatttgccat catcgatgca gtaaatggtg aagagtacct ttctggttta gccgaatgtt
     6301 tccacctgcc cgaagcacgt atcaaatgga aaatggacgg caataaacag gaagcggctt
     6361 taggtgcagg cgccgctcac agcgaagcgc tcaactttat cgttaatact attctggcac
     6421 aaaaaccaga actgtctgcg cagctgactg ctatcggtca ccgtatcgta cacggcggcg
     6481 aaaagtatac cagctccgta gtgatcgatg agtctgttat tcagggtatc aaagatgcag
     6541 cttcttttgc accgctgcac aacccggctc acctgatcgg tatcgaagaa gctctgaaat
     6601 ctttcccaca gctgaaagac aaaaacgttg ctgtatttga caccgcgttc caccagacta
     6661 tgccggaaga gtcttacctc tacgccctgc cgtacaacct gtacaaagag cacggcatcc
     6721 gtcgttacgg cgcgcacggc accagccact tctatgtaac ccaggaagcg gcaaaaatgc
     6781 tgaacaaacc ggtagaagaa ctgaacatca tcacctgcca cctgggcaac ggtggttccg
     6841 tttctgctat ccgcaacggt aaatgcgttg acacctctat gggcctgacc ccgctggaag
     6901 gtctggtcat gggtacccgt tctggtgata tcgatccggc gatcatcttc cacctgcacg
     6961 acaccctggg catgagcgtt gacgcaatca acaaactgct gaccaaagag tctggcctgc
     7021 tgggtctgac cgaagtgacc agcgactgcc gctatgttga agacaactac gcgacgaaag
     7081 aagacgcgaa gcgcgcaatg gacgtttact gccaccgcct ggcgaaatac atcggtgcct
     7141 acactgcgct gatggatggt cgtctggacg ctgttgtatt cactggtggt atcggtgaaa
     7201 atgccgcgat ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg
     7261 atcatgaacg caacctggct gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta
     7321 cccgtcctgc ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc
     7381 gcctgactgc ctgatttcac accgccagct cagctggcgg tgctgttttg taacccgcca
     7441 aatcggcggt aacgaaagag gataaaccgt gtcccgtatt attatgctga tccctaccgg
     7501 aaccagcgtc ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg
     7561 cgttcgtctg agcgttttca aacctatcgc tcagccgcgt accggtggcg atgcgcccga
     7621 tcagactacg actatcgtgc gtgcgaactc ttccaccacg acggccgctg aaccgctgaa
     7681 aatgagctac gttgaaggtc tgctttccag caatcagaaa gatgtgctga tggaagagat
     7741 cgtcgcaaac taccacgcta acaccaaaga cgctgaagtc gttctggttg aaggtctggt
     7801 cccgacacgt aagcaccagt ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa
     7861 tgcggaaatc gtcttcgtta tgtctcaggg cactgacacc ccggaacagc tgaaagagcg
     7921 tatcgaactg acccgcaaca gcttcggcgg tgccaaaaac accaacatca ccggcgttat
     7981 cgttaacaaa ctgaacgcac cggttgatga acaggggcgt actcgcccag atctgtccga
     8041 gattttcgac gactcttcca aagctaaagt aaacaatgtt gatccggcga agctgcaaga
     8101 atccagcccg ctgccggttc tcggcgctgt gccgtggagc tttgacctga tcgcgactcg
     8161 tgcgatcgat atggctcgcc acctgaatgc gaccatcatc aacgaaggcg acatcaatac
     8221 tcgccgcgtt aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc tggagcactt
     8281 ccgtgccggt tctctgctgg tgacttccgc agaccgtcct gacgtgctgg tggccgcttg
     8341 cctggcagcc atgaacggcg tagaaatcgg tgccctgctg ctgactggcg gctacgaaat
     8401 ggacgcgcgc atttctaaac tgtgcgaacg tgctttcgct accggcctgc cggtatttat
     8461 ggtgaacacc aacacctggc agacctctct gagcctgcag agcttcaacc tggaagttcc
     8521 ggttgacgat cacgagcgta tcgagaaagt tcaggaatac gttgctaact acatcaacgc
     8581 tgactggatc gaatctctga ctgccacttc tgagcgcagc cgtcgtctgt ctccgcctgc
     8641 gttccgttat cagctgactg aacttgcgcg caaagcgggc aaacgtatcg tactgccgga
     8701 aggtgacgaa ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac
     8761 ttgcgtactg ctgggtaatc cggcagagat caaccgtgtt gcagcgtctc agggtgtaga
     8821 actgggtgca gggattgaaa tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg
     8881 tctggtcgaa ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg aacagctgga
     8941 agacaacgtg gtgctcggta cgctgatgct ggaacaggat gaagttgatg gtctggtttc
     9001 cggtgctgtt cacactaccg caaacaccat ccgtccgccg ctgcagctga tcaaaactgc
     9061 accgggcagc tccctggtat cttccgtgtt cttcatgctg ctgccggaac aggtttacgt
     9121 ttacggtgac tgtgcgatca acccggatcc gaccgctgaa cagctggcag aaatcgcgat
     9181 tcagtccgct gattccgctg cggccttcgg tatcgaaccg cgcgttgcta tgctctccta
     9241 ctccaccggt acttctggtg caggtagcga cgtagaaaaa gttcgcgaag caactcgtct
     9301 ggcgcaggaa aaacgtcctg acctgatgat cgacggtccg ctgcagtacg acgctgcggt
     9361 aatggctgac gttgcgaaat ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt
     9421 gttcatcttc ccggatctga acaccggtaa caccacctac aaagcggtac agcgttctgc
     9481 cgacctgatc tccatcgggc cgatgctgca gggtatgcgc aagccggtta acgacctgtc
     9541 ccgtggcgca ctggttgacg atatcgtcta caccatcgcg ctgactgcga ttcagtctgc
     9601 acagcagcag taatctcgtc atcatccgca gctttgcgct gcggatatct gaaccggaaa
     9661 taatcactat ttccggtttt ttattctctt aatctgcatt aatcctttct gattatcttg
     9721 cttaactgcg ctgcatcaat gaattgcgcc atttcacttt gcatacttac cactttgttt
     9781 tgtgcaaggg aatatttgcg ctatgtccgc aatcactgaa tccaaaccaa caagaagatg
     9841 ggcaatgccc gatacgttgg tgattatctt ttttgttgct attttaacca gccttgccac
     9901 ctgggtagtt ccggtgggga tgtttgacag tcaggaagtg cagtatcagg ttgatggtca
     9961 aacaaaaaca cgcaaagtcg tagatccaca ctcatttcgc attctgacta acgaagcagg
    10021 cgaacctgag tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc cgggcctgat
    10081 gaacttcccg tttgaaggat taacctcagg atcgaaatac gggacagctg ttggcatcat
    10141 catgtttatg ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag gaaccattga
    10201 taacggtatc ctggcgctta ttcgccatac ccgcgggaat gaaattctct ttattcctgc
    10261 gctgtttatt ctgttttcac ttggcggcgc ggtatttggt atgggagaag aggccgtcgc
    10321 ctttgccatt atcatcgcac cgctaatggt ccggctgggc tatgacagta ttaccaccgt
    10381 cctggtgacc tatattgcca cgcaaatcgg ttttgccagt tcgtggatga acccgttttg
    10441 tgtggtcgtt gctcagggga ttgccggcgt tccggtgctt tctggctccg ggttgcgcat
    10501 cgtggtgtgg gttatcgcca ctctgattgg cctgatcttt accatggtgt acgcctcacg
    10561 agtgaaaaag aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa
    10621 gcaggctgat gttgaacaac gtccgtttac ctttggtgac tggctggtat tgattgtcct
    10681 gaccgccgta atggtctggg tgatttgggg cgtgatcgtt aatgcctggt ttattccaga
    10741 aattgccagc cagttcttca ccatgggtct ggtgattggc atcatcggcg ttgttttccg
    10801 ccttaacggc atgacggtta ataccatggc ttcatccttt accgaagggg cgcgaatgat
    10861 gatcgcccct gccctgctgg tgggtttcgc caaagggatt ttgctgctgg tcggtaatgg
    10921 tgaagcaggt gatgccagcg tgttaaatac catcctcaac agcattgcca atgccattag
    10981 cggtctggac aacgctgtcg cggcctggtt tatgttgctc ttccaggcgg tatttaattt
    11041 ctttgtgaca tccggttctg gtcaggcggc gttaaccatg ccgttactgg caccgcttgg
    11101 cgatctggtc ggtgttaacc gtcaggttac cgtgctggca ttccagtttg gtgatggctt
    11161 cagccacatc atttacccaa cctcagcttc gttaatggcg acgctcggtg tttgccgggt
    11221 ggacttccgt aactggctga aggtgggcgc gacactgctt ggactgctgt ttattatgtc
    11281 cagcgtcgtg gtgatcggcg ctcagttgat gggctaccac taaaaatgtt aagagccgca
    11341 attgcggctc ttttttcatt ctgccgtttc agtctctacg gcttcatttt tggcattgcg
    11401 cttcatccac aacgccagcg ctttcagcga gtctggagtg aactcatcgc agcgtgcggt
    11461 gatttcttcc ggcgtcagcc agcaaacttc actgacttca tcttcctgta gggcgaaggg
    11521 accgtgagag acgcagctga acaacgcgcc ccagacacgg caatttttat cttcgaaata
    11581 gaactgcccg tgctcggcaa aggggacacc ggcaatgccc aactcttctt ccgcttcgcg
    11641 acgcgcggat tccagcagtt gctcatcggc ctgaaccaca ccgcctgcgg tcgcatctaa
    11701 catgccgggt aaaaagtctt ttgtctcggt acgacgctgg accagaattt tgcccatgcc
    11761 atcatgcacg acgatgtaag ttgcacgatg acgcagacac tgtgcccgca tttgttcccg
    11821 gctggcttgt gcaatgactt cgttctcttc attgacaata tccacccatt cagtacttgc
    11881 caaacgacgc tgttccatta tcaggaaacc ttctttttct ggcgctctta cggcgcattt
    11941 tgagttgtgg gtaaattacg gattaatcgc gacctgcgca atgatacttt gatcattgag
    12001 tgcgattaca ctaagtacgt cattatccag catgccataa ctcgccggat taccgccttt
    12061 cggaatactc accgagccgg ggttgaaatg gaaaatctcc ccccgctgtt ctgccactgg
    12121 cagatgggta tgaccgtaca ccagcacatc gttctggttt aaagcaggta gattttccgg
    12181 gccaaaaaga tgaccatgcg tcaaaaacag acgttgtttt tccagtaata cctgttgcca
    12241 cggcgcggtt atcgggaaat gcagcagcat ttgatccact tcgctgtcgc agttgccgcg
    12301 cacagcgata accttatgtg ccacttcatt aagccgttca gcgactttgg ctggcgcgta
    12361 accctccggt aaagcattac gcgggccgtg attcaacacg tcgccaagga tcaccagcca
    12421 ctgggcaccg ctttgggcaa acaactccag aacacgttcc gtcgccggta acgacccatg
    12481 aatgtccgat gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg
    12541 atactggatt ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca ataaaacgct
    12601 ggacagacgc tcgctgccac tggaatgtcg cataatccac caggcgttct ggcacctcat
    12661 cgccatgtag taccaggcgg ttaatcatta gcgccagatc agtatcagca atgcaccatt
    12721 caccaaataa attcggctga cccagtacta acaaatgttc tgccatcgca aacagtttct
    12781 ctgcactggc ttttccctcg gccgttagtg gcgctttttt cgcccccgca aagacaacat
    12841 ccgtcggacg ctcttcgcgg atgggcatca gatcgctgcg cagccaggcc tgaatctgtc
    12901 gtgcacgcgc acgattttct aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa
    12961 atcgatcttc cagatactcc gcaatggcag aagattcact caactcaaaa tcatcgattt
    13021 gtaataacgg cacacggcgt gtctgaccgt aaccttgcca cgtcggctgc aaatgttcac
    13081 cgctgtcgag gtcgatggtc ttgatatgaa acgacaggcc tttttcctgc aacgccaccc
    13141 aggcggataa cacataaggg gagaaaaagt gggcatctga ccaaagcgtg atagcaggtt
    13201 tactcattat gtcctcggca ataaaggggg ctggctatca aactatagag cctttttgtc
    13261 actgtcacgt gacgaatact cacgtaagtc gccgcgcttt ctatacttgt ggtgttcaga
    13321 tgattaaaca accggagctg caatgatcga tctctatttc gccccgacac ccaatggtca
    13381 caaaattacg ctgtttctcg aagaagcaga gcttgattat cgcttgatta aggtagacct
    13441 ggggaaaggc ggtcagtttc gtccggaatt tttgcgcatt tcgcccaaca acaaaattcc
    13501 ggcaattgtt gatcattctc ctgccgatgg cggcgaaccg ttaagcctct ttgagtctgg
    13561 tgccattttg ttgtatctgg ctgagaaaac aggactcttt ttgagtcatg aaacgcgtga
    13621 gcgcgccgcc acattacagt ggttattctg gcaggtaggc ggactggggc cgatgcttgg
    13681 gcaaaatcat cattttaatc acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta
    13741 tcaggttgaa actcagcgtc tttaccatgt actgaacaag cggctggaaa actcgccctg
    13801 gctgggaggc gagaactaca gcattgcgga tattgcctgc tggccgtggg ttaatgcctg
    13861 gactcgccag cgaattgacc tcgcaatgta tccggcagtc aagaactggc atgagcggat
    13921 ccgttcgcgc cctgccaccg ggcaggcact gctaaaagca caactcggtg atgagcgttc
    13981 ggatagttaa cagaaacagg ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa
    14041 cctgcaatgg cacaacctgc cgctattatt cgtataaaga accttcgttt gcgtacgttt
    14101 atcggaatta aggaagaaga aattaacaac cgtcaggata ttgttatcaa tgtgacgatc
    14161 cactaccccg ccgataaagc gcgcactagc gaagatatca acgatgcgct gaattatcgc
    14221 accgtaacga aaaacattat tcagcatgta gagaataacc gtttctcttt gctggaaaaa
    14281 ttaactcagg atgtgctcga tatcgcacgt gaacatcact gggtgacgta tgctgaagtg
    14341 gagatcgata aactgcacgc gctgcgctac gccgattcgg tatccatgac cttaagctgg
    14401 cagcgttaac cgccatatcg ggaggctgca tgaacatagt gatcaccgga gggacgggat
    14461 taattggtcg ccatttgatt ccgcgtttgc tggagctggg ccatcaaatc acggtagtga
    14521 cgcgtaaccc acagaaagcc agttccgttc tcggccctcg ggtgacgcta tggcaagggc
    14581 tagccgatca aagcaacctc aacggcgttg atgcggtaat caacctggcc ggagaaccga
    14641 ttgctgataa acgctggact cacgagcaaa aagagcgtct ctgccaaagc cgctggaata
    14701 tcacgcaaaa actggtcgat ttgattaatg ccagcgacac gccgccgtcg gtactcattt
    14761 ccggctcggc aacgggctat tatggcgact taggtgaagt ggtggttacc gaagaggaac
    14821 cgccgcataa cgaatttact cataaactct gcgcccgctg ggaagagatt gcctgccggg
    14881 cgcaaagtga caaaacgcga gtgtgcctgc tgcgaaccgg cgtggtactg gcaccggacg
    14941 gcggtattct cggtaaaatg ctgccaccgt ttcgtcttgg cctgggcggg ccgattggtt
    15001 ccggtcggca gtatcttgcc tggattcata tcgatgatat ggtcaacggc attctctggc
    15061 tgctggataa cgagctgcgc gggccattta atatggtttc gccctacccg atacgcaatg
    15121 aacaatttgc ccatgcgctc ggtcatgcgc tgcatcgccc ggctattttg cgcgtccccg
    15181 caaccgccat tcggctgtta atgggcgaat cttcagtact ggtattaggt ggacaacgcg
    15241 cgctgcctaa acggctggaa gaagcgggtt ttgcgtttcg ttggtacgat ttagaagagg
    15301 cgctggcgga tgtcgttcgc tgatgtggtt tactgcaaac atccgccagt taactcccgg
    15361 tgttacaggg ttagtggctt tgcgctataa gatcgtctgg cgatagtcgg gtcaccatca
    15421 taactaactc tctgtctaaa cctctatcca gcatctcctg agcaatacgc agggcttctt
    15481 cgcgtttgcc cagaattaat ccatcattct gcccttcttc acgtaatctg tcagcaatgg
    15541 tcatcagttt ctccttttct tgtggtgcgc gttctgctat ctcaccaata aatgcacgga
    15601 aacgctgggc atctcccgtt tgtaatacat aattaaacag ggcttttagc tgtctgtcat
    15661 tagtgttccc tgtaactagc agcgaaacaa tttggtcgac taatcccaac agatcgcgct
    15721 gacgaatatg tttctgaatt aactccaaca gcgccatttt gcggtgttgc ataatctcgt
    15781 catccggcac cacggtaata tccaccaacg gaaaagccga tgaatatatt ttacgggcaa
    15841 tcgcaggctc agcaaattca tcaagccagc agagtgaata aggataagga cttctgcaac
    15901 catgataaaa cagcatcggg atcaccaatg gaagctcttt atagcccgca tcaagatggt
    15961 tttgcattgc cgcaatggaa taacgcatca tgcgaaaagc cattaattct tccggcttac
    16021 tttggtgctc tatcactaca taaatataac ccactccctc ctgcgttttc acagaccaca
    16081 agaggtcgga ataatattgc cgcaggtcgt catcaataaa actgtttggt tccagtttaa
    16141 gcgtcgttaa atcacacagt ttgcgcagcg gcgcgggaag atgaatatca ataaaatccc
    16201 gcgcggtgtc tggatggcgt aaaaaagatt taaataccgc atcatgcggc gtggaagttg
    16261 tcgatattgt catggcgttc cgtcacccct caaatttaag atgacgcgac aataaccacg
    16321 attaacaccc aatgcagcag gcagttttta tctttggaca gcgaattcag aagtattttt
    16381 agcggactgc aacttttaca tttcagcgga agctgactcg caaattcccc atgaaaatac
    16441 tccggcagcg ttagccaccg gagaggaaat tatttcagcg atcccttaag gaactgttgc
    16501 agacgagggc tttgcgggtt gccaaataac tgctccggcg cgccctcttc ttctattttc
    16561 ccctgatgga ggaaaatgac gtgagtagaa acatggcgag caaagcccat ttcgtgagtc
    16621 actaccacca tggttttccc ctcttctgcc agttgctgca taatacgtaa cacttcgcct
    16681 accagttcag gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc cggttccatc
    16741 gccagcgccc gcgcgataga aacacgctgt tgctgaccgc cggaaagatg caccggatat
    16801 ttcccctgcg cacgttcgtc tatcccgact tttgccagat acttcaccgc ccgctcgcgc
    16861 gcttcctgct tgctcaggcc caacacctga atcggcgctt ccatgacgtt ttccagcacc
    16921 gtcatatggc tccagagatt gaagtgctgg aataccatcg tcaggcgtgt gcgcagtaag
    16981 cgcagttgat ttttatcggc gactttgagt tgaccgtctt tgtcgcgcac cagattgatc
    17041 gtctggccat tgaccacgat cgacccttca ctcggttttt cgaggaagtt aatgcagcgc
    17101 agaaaggtac ttttccccga tcccgacgat ccgatgatgc ttattacatc tccggcattc
    17161 gcttgcagtg agaccccttt cagcacttca tgttcgccgt agcgtttgtg caaatcgata
    17221 acgtttaatt tattctcgga catcgtattc tcagtgcgtt gaagaaggtt tcacatgctg
    17281 caaccagcgt ttttccgctc tgcgaaagag gctgatcagg acataagaga tgattaaata
    17341 gagcaccgcg gcaatgccga aggcggtaaa aggttgatac gtggcggcgt taatatcgcg
    17401 ggctattttc agcagatccg gcacagtggc cgtaaacgcc agcgcagtag agtgcagcat
    17461 caggatcact tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag gcaaaataat
    17521 gcaacgatac attttaaaag tcgagaagcc ataggctctg gcggcttcaa tttccccatg
    17581 cggaaccgaa cggattgccc cagcaaaaat ctcggtagtg taagcgcagg tgttaagcgt
    17641 cagcgccagc acggtacagt tcaggccact gcggaagaaa gcgttaagga attcggttcc
    17701 cttaacaatc tcaagcgtgt acatgccgga atagaacacc agcaactgaa catacagcgg
    17761 cgtaccgcga aaaatatagg taaataacca gattggaaac tggatgtatt tattactgga
    17821 gacacgacca atcgccagaa acagcgccag gactccgcct atcactaccg acaaaataag
    17881 cagccacaga gtgatcgcca caccagtaaa gcgataaccg tcggtccaca gcagcggttt
    17941 ccagtattca tgtaagattt cgatcacagg tcagccctct tcacacccac ggagtagcgg
    18001 cgctcaagga acagcagcac accattggaa acagtggtga aaaccaggta aatcacgcca
    18061 cagacgatgg cgaaatagaa cggttcccag gtacttttgc ctgccagttg ggtggctttg
    18121 accacatctt ccaggccgag taacgaaacc agtgcggtag atttgaggat cacctgccag
    18181 ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat ccgccgaaac
    18241 acttgcccac gagtaaaacc gaacgccgtc gccgcctcta tatgcccttt cggcactgcc
    18301 ataaacgcgc cacggaacgt ttcggtaaaa taagcaccgt agataaaacc gagagtaata
    18361 ataccagcga ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc cgtcaccgtg
    18421 tttagtgcaa tctgcaaacc gtagaaaatc agcagcatca acaccaagtc cggcacgcct
    18481 cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg cgagagctta
    18541 ccgccagcgc caattaaacc gatgattaca gcgagcacta cagaactgat agccagctcc
    18601 agcgtgacga gcgcaccctg taaaataaca cctgaaaacc catacaacat gctgcctgtc
    18661 ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg gttacccgaa gggagcgggc
    18721 tttctttcac cgatggcgat taaccgccat aaacatcaaa atcgaagtac tttttcgcta
    18781 atttctcgta agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt gcctcgcgca
    18841 gttcgttatc ttctttacgc aggcccatgc cggtccctac gccaaacagt ttttcatctt
    18901 taacagacgg gccaccgaat ttgtaatctt taccgacagg ttgtttgagg aaaccttcgc
    18961 tggcagcgac ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg tcagaataaa
    19021 tgttgtcctg cccctgatac gagacgattt caatgccttt tggtgcccaa tgttcattac
    19081 cgaacgtctc ctgggtggtg ccctgcaata cgcctacccg tttgcctttc agcgactcga
    19141 ctgtcggctg aatgtcggaa tttttcgcca ccaccaaacg agaatcggca gcgtacagtt
    19201 tgtcggtgaa ggctatttct tgctgacgtt tttccgtaat ggaaagcgat gacatgatgg
    19261 cgtcaatctt cttcgctttt aaggacggga ttaacgcatc cagcggattt tcgacaaacg
    19321 tacattgcgt attgatgcgt ttgcataatt cctttgccag atcgatatcg aagccaacca
    19381 gttcgccttg tgaatttttt gattcaaatg gcgcataggt cgggtcggta ccgatgcgga
    19441 tgttttgcgg aatcgcagca aacgccgcag ttgcgctgga gaaggccaga accagagaga
    19501 gcgatagcac cagttttttc ataatgtcct caactgacag tctttttata gggaatattt
    19561 acaggtttgt tacacttatc gtgccataaa attgaccatc aaggcaaaat atcccgcccc
    19621 gttagacgta ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc accacgatag
    19681 tgcaacaaac ctggcgtgca ccatgatgat cattccatca ggtacagctt cccagcgacg
    19741 tatcagtcac cgtagacatt aaagtcgaaa tactttttcg ccatcttgtc gtaggtgccg
    19801 tcctgacgca gctcgccaag cgccttattg aaggcagccg tcagttcagc atcatcttta
    19861 cgtagcccta caccggtgcc atcaccgaag tattttttgt cttttactga tgagccagca
    19921 aaggcgaaat ctttaccagc aggttgcttg aggaatcctt cgctggcagc aacttcatct
    19981 tgtaacgcag catccagacg tcctgcagcc agatcggaat agaccaaatc ctggttggca
    20041 taggccacca catccacgcc tttgctacgc caggtctcgt tagcgtaagc ttcctgggtt
    20101 gatccctgca gcacaccaac atgtttacct ttcagtgaat ccagcgttgg ctgaatcggt
    20161 gaacctttgg cagcaatcaa acgagaatct gcggcgtaca gcttgtcgga gaaggcaatc
    20221 tcctgctgac gtttatcggt aatggaaagc gacgaaataa tggcgtcgat ttttttcgct
    20281 ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt cacctgcatc
    20341 cgtttgcaca tctcgttacc gagatcgata tcaaagccaa caaaatcacc tttagcatct
    20401 ttcgacgaga acggcgcata ggtggtatcg gttccgatac gtaccgtctc cggtagcgcc
    20461 gcatagctgg aagccgctgc ggagagaccg actaacaaag acagagcgag aatcgacttc
    20521 ttcatacata accctcaagt gaaaatggct tttttatgtt gtgtattgtg ttgtgtgttt
    20581 gcaggctctt tcatgcaggt cttatgccat cttgacgaca acagtaacat tcaacgttaa
    20641 atatgttaat aagacgttgc attattgtcc tgaagttgaa gatagcaggt atagcggttg
    20701 aatcgcagcg tttcgattga atggcagaaa caaaatgtcg aggatttgat cgcggttgca
    20761 aaattgccct gaaacagggc aacagcggag ttatgcgccc tgccagcggg caaagagatc
    20821 ttcaggaagg gttattgcaa actggtcaag aacacgatta accgtctgat ttatcacatc
    20881 atcaagggat tgcgggcgat gataaaacgc cggaacggga ggcataatca ccgcaccgat
    20941 ttctgccgcc tgagtcatta aacgcagatg gcctaagtgc aatggtgttt cacgcacgca
    21001 gagcaccaac gggcgacgct ctttcagcac cacatctgcc gcacgggtca gtaagccatc
    21061 agtatagcta tggacaatgc cggaaagggt tttgattgaa cagggtaaaa tcaccatccc
    21121 cagcgtctgg aaagaaccgg aagagatgct ggcggaaata tcgcgcgcat cgtgcgtgac
    21181 atcagctaat gcctgcactt cgcgcaggga aaaatccgtt tcgagggata aggtctggcg
    21241 cgccgcctgg ctcatcacca gatgcgtttc gatatctgtg acatcgcgca gaacctgtaa
    21301 taagcgcacg ccataaatcg cgccgctggc accgctgatg cctacaatga gtcgtttcat
    21361 aaatagttgc cctgtcagac ttgcaggcag actttgcagt atttcggcgg gagttgcaag
    21421 tcagggtgcc agaccggcac cctcagcgaa ggcatcatcc ttcgttatgc atttcgagat
    21481 tttccacttc gttctgacgt tgcactgctt tggcgtcatc attacgtaac gtatcgagga
    21541 aatcgaggta gccctgatca acatctttgg tgacgtagac gccgttgaac accgagcatt
    21601 caaactgctg gatatccgga ttttcagcgc gaacggcgtc gatcagatcg ttcagatcct
    21661 ggaaaatcaa cccgtcagca ccgatgatct ggcgaatttc atcaacttcg cgaccgtgag
    21721 cgatcagttc cgtggcgctc ggcatatcaa taccataaac gttcgggaag cgaatttccg
    21781 gtgccgcaga agcgaggtac actttcttcg ctccggcttc gcgtgccatc tcgataatct
    21841 gctcagaagt ggtgccacgg acgatggagt cgtcgaccag caggacgttt ttatcgcgga
    21901 actcggcgcg gttggcattc agtttacggc gcacggactt acgacgcagc tgctggcccg
    21961 gcatgataaa ggtgcggcca acatagcggt ttttaacgaa gccctggcgg tacggtttgc
    22021 ccagaatacg agcaatttcc agcgcgatat cacacgaggt ttctgggatc gggatcacca
    22081 cgtcgatatc cagatcttcc cattcgcggg caattttctc gcccagtttc gtgcccatat
    22141 tcacacgcgc gctgtaaacg gaaattttgt cgatgaacga gtccgggcga gcaaagtata
    22201 catactcaaa caggcacgga ttactgaccg gattgtcagc acattgacgg gtaaacaact
    22261 gcccttcttc agtgatgtaa atcgcttcgc ccggcgcgac gtcacgcagg aaatcaaagc
    22321 ccagcgtatc gagcgctacg ctttcggaag cgaccatata ttctgtacgg ttctcgtcaa
    22381 tatcacgttt tcccagtacc agcggacgaa tcccgtttgg atcgcggaaa gcaaccatac
    22441 cgtggccgat aatcatcgcc acacaggcat atgcgccgcg gattaagcgg tttgtggcag
    22501 caatggcagc gaaaatattg tcggcttcca gcgggtagtg gcggaagttg tccagctcgc
    22561 tagcgaagat attaagcaga atttccgagt cggaagtggt gttgatgtgg cggcgttttt
    22621 cttcaaacag ttttttacgc aactcgtgag cgttggtcag attgccgttg tgggcaagcg
    22681 taatgccata cggggagtta acgtaaaacg gctgcgcttc agaggcgctg gagctgccag
    22741 ccgtagggta acgcacatga ccaatgccca tattgccctg caaacgctgc atatggcgag
    22801 cttcaaatac atcgctcacc agcccgttcg ctttacgcaa acggaagcag ttattggcat
    22861 ctatggtgat gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt aaggcatcat
    22921 aaatcgactg gttaaccggc ataacaccgg cgataccgac aataccgcac atacgtcttt
    22981 ttcctcgtta agccacatct cagagcactt acgctctggg caagaaactt gacgagcttt
    23041 gcagataatc aaaaaaccat ctgatgataa aactgaactg cgggatcagc tgtgatttgc
    23101 tccagtcttc gcttttcgac accccggtaa aggagtcgag aaagaataga atggcggcaa
    23161 caatcaacac accgcgcaac gcaccgaaac agacgcccag cacccgatcg gtgcctgaca
    23221 accccgtttt ctccaccagc tggcctatca cgaagttcac gatagcacca acgatcaggg
    23281 tagcgataaa tagtaccgcg atggcaatcc catttcgaac cagttcgtct tcaaagcccg
    23341 taaaccagac tgacaggtaa gtgtagtaat gactggcaac aaagaaagca caaccccatg
    23401 tcaccagcga taacgcttca cgaacaaagc cgcggatcag gctaaccaga gaggaaaaag
    23461 caatcaccgc gattatggcg taatcaatcc agaccatatg tgtcccacga ttttacgccc
    23521 tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta gggatttcct
    23581 tcccgcgcat caataaaaat ggcgctgaaa aaatattcaa cgccatcgac tttttatgcc
    23641 tttgcggcat cgtgcaatgc gtgtcggatg cggcgtaaac gccttatccg acctacggtt
    23701 ctaaccctgc ccagtcctga taagacgcgc cagcgtcgca tcaggcaagg ccgtattaat
    23761 tcggcgtata gcccattacc acgccactta agccagaaag ttgcttcaac tcacccagcg
    23821 aacctttcag cttatctttc gaggcatccg gcccaaccag aatacgggta attttaccct
    23881 gcactggcgt ggatggcgac gtataaaccc gataaccggc accgcgcagc ttaccgacaa
    23941 tctcatttac tttatcggca tttttcagcg cacccagttg cacaacatag gctttacccg
    24001 ttggcgctgc tttttcttcc acgaccggct tcggttctgg cttcggcgca ggtggtgctt
    24061 caaccttcgg tttaggcggc tccaccggtt tcggcttcgg tggggcgacc ggtgcaggtt
    24121 ccggttcaaa ctcggtgtta ttggctgcaa tagtggccgg atcgagcgac ggtgctgcgg
    24181 catcacctgc ccgcacctct tccgctgcgc cttccggcgg ctgcgtcggc aacgcctggg
    24241 tggcggctgg catcatatca ggctcatcac gatcgcccgc tttcggcacc agcgggatag
    24301 ccgcgaactc atcctgataa tgttttttct gcccgtccag cagccctgga agtacaatca
    24361 cccccagcgc caccagcacg atcgtgccca ctaaccgatt ctgaaactta cttgccaccg
    24421 cttctcctcg cgtcaatcac ttccatgaca tgtgcgaccg tgtggaaaga accacacacc
    24481 agcacggtgt cttccgcttt agcgtccgcc attgcggcat cccatgcctg cgcaacgcta
    24541 tcaaatgatt tgccgttacc caaatgctca agcagttgtt ctgccgtggc accgcgcggc
    24601 ccttccagtg gcgcacaata ccagtcatca accacgcttt tcaaccaggc cagagttccg
    24661 gcaatatctt tatcatgtag cataccgata accgccagca cgcgcccgtt tttcggtagc
    24721 gctttcatac gcccggtgag atattccgcc gcatgtggat tatgcgcgac atcaaaaata
    24781 acgcgtggcg actcgctcac aatctggaaa cgtcccggca aaattgcgct ggcaatcccg
    24841 tcgcgaatgg cattttcact gacttccagc ccgctggcac gcagtgccgc cagcgctgtt
    24901 gcggcattcg gttgcgggac aagcggcaac ggcagatttt ccagcgtgcc gtgagcatcg
    24961 ctaaacgccc agtcatgatc ggtgacggaa tagttccact caacgccccg acgttgtaac
    25021 agtgcacctt tttcctgcgc cacatcagca atggtagaag gcatttccgg ctcaccgaca
    25081 attgccggtt ttgcgctgcg aaagatgcct gctttctcgc gaccaatact ttcgcgatct
    25141 ggtcccagcc agtcggtatg atccagcgca atgctggtta ctacagccac atcggcatcg
    25201 acaatattgg ttgcgtccag acgaccgccc agccctactt ccagaatcac cacgtcaagt
    25261 tgtgcctgct tgaacagcca caacgccgac agcgtaccgt actcgaaata ggtcagggaa
    25321 atatcaccgc gtgccgattc aatctccgca aaagaggcgg tgtgggccga ttccggcaat
    25381 tcctggccct gcacacgtac gcgctcggta taacgcacca gatgcggtga actgtagacg
    25441 cccactttgt accctgccgc catcagaatc gactccagcg tacggcaggt ggtgcctttg
    25501 ccattcgtac ccgcaacggt aaacacaaat ggcgctggtt tcagaacgcc aagacgcgcc
    25561 gcgacctggc tcacgcgctc aaggccgaga tcgatagttt tactgtgcag gttttccaga
    25621 taagaaagcc acgaagccag aggcgacgcg gcttgaggag tgcgtttgat aatcatggta
    25681 tccgctgatt cgttacggtg agaatagcaa aagggcagag ccagtggccc tgcccttatc
    25741 agttatcagg cctcaggttc ctgatccggt accgggggta ccactacgcc ttcacgcggc
    25801 gcttcaggat tcggcgctgg cagattcatc aacttcgcca gaatgctcgc cagtttcagg
    25861 cgcatttccg gacgacggac gatcatgtcg atcgcgcctt tctcgatcag gaattcactg
    25921 cgctggaatc ctggcggcag tttttcgcga acggtctgtt cgataacacg cggaccggca
    25981 aagccgatta acgctttcgg ttcagcgatg ttgagatcgc ccagcatggc gaaacttgca
    26041 gaaacaccgc ccatcgtcgg gtcggtcagc acggagatgt acggcaagcc gcgctcctgc
    26101 atttttgcca gtgccgcaga ggttttcgcc atctgcatca gcgacatcag tgcttcctgc
    26161 atacgtgcgc caccagaggc ggagaagcag atcagtgggc agttatcttc cagcgcctgc
    26221 tcaacggcac gcacgaaacg tgcacccaca acagacccca ttgaaccgcc cataaaggcg
    26281 aactcgaatg ccgcagcgac aaccggcatt ccatacagag tgcctttcat caccaccagc
    26341 gcatcttttt cgccagtttc tttctgcgca gatgccagac ggtctttata cttcttggag
    26401 tcacgaaact tcagcacatc tttcggctca agctcgctac ccagctccac aaggcttcct
    26461 tcatctaaca ggctatgcag gcgattacgc gctgtcatac gcatgtgatg gtcacacttc
    26521 ggacagacct caagattacg ttccagctca gcgcggtata aaacctgacc gcagctatca
    26581 cacttagtcc acaccccttc aggaatgctc gccttgcggg tgggagtaat gttgctttta
    26641 attcgttcaa tccagctcat tagggacctt tctgtctgaa cctggttcga tgccagtttt
    26701 atctttgggg acgcataatg ccatttttgc ccccaacaga ccatgaatgt tgcacattaa
    26761 aacataacag cccgaaactt tggataaaaa agtggtcgaa ccgcggagtt actttttatt
    26821 ttgcggcgcg tgccgcagcg cgtttgtgac gaataaattc gaaaacccca ggcaatattg
    26881 aaacaaaaat aatgccaaca atcatcagct taaggttgct ctgaatgaag gggagcgtgc
    26941 cgaagaaata acccgcgtag gtaaaaatca gtacccacaa cagtgcgccg atcacgttat
    27001 aagcggcgaa atgacggtac gacatgtggc ccattcccgc aacaaacggc gcgaacgttc
    27061 tgacgatcgg tacaaaacgg gcgagaataa tcgttttgcc gccatgtctc tcgtaaaact
    27121 gatgagtctt atcaagataa ctgcgacgaa agattttcga atccgggtta ctgaacagtt
    27181 tgtcaccaaa taaccgtcca atggtatagt tcactgcatc accgacaata gcggcaatca
    27241 acataagcac caccatcata tggacattga gatcgttagt ttccagcgac gccaatgcac
    27301 cagcaacaaa cagcagcgaa tcaccaggca ggaatggcgt taccaccagc cccgtttcgc
    27361 agaacagaat caaaaacaga atggcataaa cccagacgcc gtactccgcg accagctctg
    27421 ccagatgcac gtcaatgtgc aggataaaat cgatgaggaa ataaatcagg tccatatttg
    27481 cctatgcctt gtactcgtca tctttcaggc tgtaactacg ttggctacag cctgaaatgt
    27541 tccgagcatt attctcgtta gtccgccaga aatagcgggc ccattggcgg ttttggaaga
    27601 tcataccggt cagggtaatc caccgcgacc agatacagcc cttccgcttt tgccgttgct
    27661 gccgccagcg ttctgtcctt tgccgccagc aactctgcta tccagctctc cggctggttg
    27721 tgggcaccga cttccatcag gctgccgaca atattcctga ccatatgatg tacaaaggca
    27781 ttcgctttga tatctaccac cacataagga ccgtgacgcg tgacattaat atgcataacg
    27841 ttacgccacg gggttcggga ctggcactgc accgcacgga acgaggtgaa atcattctcg
    27901 cccagcaagc attgcgcagc ccgatgcatc cgttcagcgt ccagcggttc gtaaaaatgg
    27961 gttacccctt tactcagtac cgccgggcgc agccgatgat tgtagatgat gtagcgataa
    28021 cggcgagccg tggcgctaaa tcgggcatga aaatcatcag gtacagcttt aacccaacgc
    28081 acggcgatgt caccaggtaa attcgcattt acgcccaagg tccacgccgc gtctttgcgc
    28141 tgcgcggtcg tttcgaaatg cactacctgc ccggtaccgt gtacaccagc gtcagtacgc
    28201 ccggcgcaga agacggtaat gggttcgttc gccacctggg agagcgcctt ttccagcttc
    28261 tcctgcacac tgcggacttc attctgccgt tgccagccgt aatacttact gccgtcgtac
    28321 tcaatgccca gcgcaatttt ataaactggc ggttgttgct ggtccgacat tagtacagat
    28381 actcctgcac cagtttctcg gcgattttta ctgccatcag cgcgccgcca aagcgaacgt
    28441 tatcggccac cgaccagaac tggacttgct ccggcatacc gtagtcatta cgcacgcagc
    28501 caacagaaag atgcggcgta cccgaagcat cccctacctg agtcgggaat tcgttctctt
    28561 cagagagcac aatatcttcg ccctgagcaa acgcatcaca cgcttcttct gccgccagcg
    28621 gacgcagtgc ttcaaagttg accatctggg catgaccgta gaataccggt gcctggacga
    28681 cgcttgccga aatcatcagc ccttcgtcct gcaggatttt gcgtacttcg tcaacgatac
    28741 gacgttcttc acgcacgcta ccttcgctat ccggcaacaa cggcagcata ttgaacgcca
    28801 gttgacgacc gaagaaatct tcttcgtcaa tcggaatgcc gttgagcaat ttcgcactct
    28861 gccccgctaa cgcatcgacc gcttttttgc cctgggcgga ggctgaaatc aggctggtaa
    28921 cactgatacg cgacaaaccg ccctgatcga ttaacggttt cagtgccgcc agcagctggc
    28981 tggtcagact gtctggtacg gcgatgacat tccggttgcg gtaatcggtc agtacaaacg
    29041 ggtttacttc cggcaccacc agcggtacgt cgggttcgag agcaaacagt ccgctgctgt
    29101 cgatcaccag gcaacctgag ttggtcgctt cttcaaccca ggcagcggta gcttctttgc
    29161 ctgcgacaaa aaatgccagc tgcgcctgcg tccagtcgaa ttcagcggca tcctgcacgg
    29221 tgattgtctt accaccaaag cgcagttgtt cgcctgcgct ttcgttacgt gccagtgcat
    29281 aaatttcccc aaccgggaac tgacgttcag ccagcgtttc aagcagggct tcgcccacag
    29341 cgccagttgc gcccaggacg gcaatgttcc agccttcaga catggtggtt tactccagaa
    29401 atagcaaagc tccctgccaa tgttacagca gagagcatga agaagagatt aacgtgccgg
    29461 atgatgaacg gcgttaaaac ccagtttaca cagcaatgat gccgcactgg cgtcatcaca
    29521 aattacatac agagatgacc attcacggcg ctcaagatag tttttgcgca gtttatcgaa
    29581 ctcacccggt atcccggcga ctttacgcag cggtgcgtca tcgcggcgca catcatacac
    29641 caaatgcacc agccttttca gcgtcggttg atcgagcggg ccatgcagcg taatgcgacc
    29701 aaactctggc gcgggcagta atgtatccag cgcaacgtgc tgttcatgcc caataaactt
    29761 gctataggct tcaaacactt gcgtagtacc gcgtgcttta ccttccaggg tataacctgc
    29821 gatatgcggc gtgccgatat ccacttttgt cagcaactcc acgttaagtt ccggttcgcc
    29881 ttcccagaca tccagcacta cgcttaactt ctggccttca ttcaggcagg tcagcaacgc
    29941 agtattatcg acgactgcgc cacggcaggc gttaatcaga atcgctcccg gcttcaggct
    30001 acggatcagt ttttcatccg ccagatgcag cgttttgtac ggcccatctt tgaagagtgg
    30061 cgtatggaaa gtcagaatat ccgcatgctg aactaattca tccagcgagc ggaaatcacc
    30121 ctcatcccca cggtcagcgc gaggcggatc gcaaagcaat gttttaatcc ctaacgcttc
    30181 cagtcgcgcc tgcaatcgac gtccaacgtt accaacgccc acgatcccca ccgtacggtc
    30241 gtgcagtgaa aatccatcgc gttcggcaag catcagtagt gaggagaaaa catattccac
    30301 caccgcaatc gcattacagc caggtgcagc ggaaaaacca attcccgcct gctttaacca
    30361 tgcttcatcg acatggtcgg tccccgcggt ggcagtgcca acaaatttaa tgggttttcc
    30421 tgccagcaaa gattcattca ctttcgtgac cgaacgcacc atcagcgcat ccgcgtctgc
    30481 cagttgagcg acggggattg gacgcccggg aaccgcggtc acctcaccca aacggctaaa
    30541 taagtcgcgg gcataaggca tattttcatc aacaaggatt ttcacgtttg tgttacctgt
    30601 atgagacgag agttaaccgg acaagtgtgc cataatctcg cggccaggca tacttgcgaa
    30661 gatttcaggt ataaggatac gtaatgatac aacctatttc cggccctcct cctgggcaac
    30721 caccaggtca gggagataac ctgccgtctg gcgcgggcaa tcagccttta tccagtcagc
    30781 aacgtacttc gctggaaagc ttaatgacga aagtgacctc actgacgcaa cagcaaagag
    30841 cagaactgtg ggcgggtatc aggcacgata ttggtctgtc gggagattca ccgctgcttt
    30901 cgcgtcactt ccctgccgct gaacacaacc tggcgcaacg tctgctggcc gcgcaaaaaa
    30961 gccattctgc ccgccagctt ttagcgcaat taggggagta tttacgtctg gggaataatc
    31021 gtcaggcggt cacggattat atccgtcata actttggtca gacgccgctg aatcagctct
    31081 caccggagca attaaaaacc attctcaccc tgttgcagga agggaagatg gttattccgc
    31141 aaccgcagca gcgcgaggcg accgaccgtc ctttattacc ggcggagcac aatgcgctca
    31201 aacagctggt gaccaaactt gcggcggcaa cgggggaacc cagcaaacag atctggcaat
    31261 cgatgctgga actttccggg gtgaaagatg gcgagttaat tccagcgaaa ctgtttaacc
    31321 atctggtgac ctggctgcag gcgcgccaga cgttaagcca gcaaaatacg ccgacgctgg
    31381 aatcactaca gatggcgcta aaacaacctt tagatgccag tgaattggcg gcgttatcgg
    31441 catatatcca gcaaaaatat ggcctttctg cgcaatcatc gctttcttct gcccaggccg
    31501 aggatattct taatcagctt tatcaacggc gggttaaagg gattgatccg cgtgatatgc
    31561 aaccgctgct taatcctttt ccaccgatga tggacacgtt gcaaaatatg gcaacgcgtc
    31621 ccgcgctgtg gatactgtta gtcgcgatta tcctgatgct ggtctggctg gttcgttagc
    31681 cccgacgaaa tgacagtatc gtgacaataa ttcccagcac cgcagagatc gccccggcaa
    31741 gaaataccga agagtaacca aacgtggtcg ccagcattcc cgccagcggc ccggagacgc
    31801 cgagggcgat atcctgaaac gcggcgtaac cgcccagtgc ggtgccgcga acttgtgagg
    31861 ggacgcgttt aaccacctcc acgcccagcg caggaaagat aagcgaacat ccggctccgg
    31921 ttaacgccgc gcccgctaat gcgacccacg cacctggggc ttgccagagc agcaacaagc
    31981 ccaccgtttc tacaagcaga gagacaatcg ccactttcac gccgccaaaa cggtccggca
    32041 tccagccaaa catgacgcgc atcacgacaa atgcgccgcc aaacgcggta agagtaaagc
    32101 ccgccatcgc ccatcctttg ctggcaaagt agagcgaaac gaaagtcccg ataaccgcaa
    32161 aaccaacgcc ttgtagtgcc agccctaacc ctggtttcca gataaacccg acaacgctcc
    32221 acagcgatgg acgttctccc gccagggccg gtactttgcg cactgtgccg ttacaggccc
    32281 acgccagtaa gggtaatacc attgtggtga tcgccagtgc ggcaaaaccg taatggctat
    32341 gaatcaacag gccaagcgga gcaccaacag cgagggcacc gtaaatcgcc attccattcc
    32401 atgacatcac tttgccagag tgttttggcc ctacgatgcc taacccccag gtcagagcgc
    32461 ctgtcagtaa ctggctttca ccaaacccaa gaatcaaacg cccgatgacc aacagggcaa
    32521 atttgaacgg tgcggagaca ggcaaaatcg ccgccagcag caacgcgccg ccagccagac
    32581 cacaagctaa catcccctga agcgccgaac gttttgcacc atattgatcg gccagtcgcc
    32641 cggcatagcc acgcgtcagc accgtagcca gaaactgaat cccgacggca atcccgacca
    32701 tggtattgcc atagcccaga tcatgatgaa caaacagcgg gataaccggc aacggcagcc
    32761 ctacggtcat gtaggtgaga aaaaccgcaa aagcgatgcg gaagagcgaa aaattggcag
    32821 aagatcgtgt ttcggtttgg cttacagcag tcatgcatta ctccagaatg cagcgcaagg
    32881 cgaggagtat ccccgtctca tctctctggt ttcagggtta cagtgcgttg gcaggattta
    32941 acgcgtacgt cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg aactggcggc
    33001 gattgtcaat gatgtgaaaa agggaaccat caggttccct tttgcgttag tgccggaggc
    33061 cgcattggcg cgtaacgtcg gatgcgacgc tggcgcgtct tatccgacct actccgacta
    33121 ctccgaccta ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg
    33181 ccgaagccga agctgttaga cataacggta gtcagttcgc gatcggtcgt ttcggtcacg
    33241 atgttcagac ccgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag
    33301 ccgtgttcca gcatcagcag agagtagata gcttcctgta cgccagcagc gcccagagag
    33361 tgaccggtca tggctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg
    33421 atagctgcca gttctttcac gtcgccaacc ggagtcgaag taccatgaga gttcaggtaa
    33481 tcgattgggg tatcaacgcc gtgcatcgcc atcttcatgc agcgtactgc gccttcgcca
    33541 gacggagcaa ccatgtctgc accatcagag gttgcgccgt agccaacgat ttcagcatag
    33601 atgtgagcac cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccgccg
    33661 ccagcgataa cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggggtg
    33721 tcgttgtatt tagtagacag cgcacccatc gcgtcgaatt cgcaggccat ttcccagcac
    33781 agctcttcgc cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact
    33841 gcgttaccga tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgcca
    33901 tgaattttaa acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc
    33961 acatacgggc caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaacacc
    34021 tggaaacgcg gggaaccgcc gccagaacct gcaatcaggc caacgcgcgg gttattctgg
    34081 taagcttccg gagaaaggcc cgcatctgcg attgcctgct ccatagaaag gaatgcataa
    34141 atggatgcgt cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt
    34201 tttacgttgc cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg
    34261 atccctgaac gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg
    34321 gaaacaatgc ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac
    34381 atagagtaag tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc
    34441 catttaatag agaaatttgc gcagccttac acacatcgct aagatcgagc caccgcctgt
    34501 aagacgagta acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc
    34561 tgagggtaca cctgtttccc gagattttga cgatgtctat ttttccaacg ataacgggct
    34621 ggaagagacg cgttatgttt ttctgggagg caaccaatta gaggtacgct ttcctgagca
    34681 tccacatcct ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac
    34741 gctatggcag gcatttgatc agtttcgcga agcgcatccg caagcgcaat tacaacgctt
    34801 acatttcatt agttttgaga aatttcccct cacccgtgcg gatttagcct tagcgcatca
    34861 acactggccg gaactggctc cgtgggcaga acaacttcag gcgcagtggc caatgccctt
    34921 gcccggttgc catcgtttat tgctcgatga aggccgcgtg acgctggatt tatggtttgg
    34981 cgatattaac gaactgacca gccaactgga cgattcgcta aatcaaaaag tagatgcctg
    35041 gtttctggac ggctttgcgc cagcgaaaaa cccggatatg tggacgcaaa atctgtttaa
    35101 cgccatggca aagttggcgc gtccgggcgg cacgctggcg acatttacgt ctgccggttt
    35161 tgtccgccgc ggtttgcagg acgccggatt cacgatgcaa aaacgtaagg gctttgggcg
    35221 caaacgggaa atgctttgcg gggtgatgga acagacatta ccgctcccct gctccgcgcc
    35281 gtggtttaac cgcacgggca gcagcaaacg ggaagcggcg attatcggcg gtggtattgc
    35341 cagcgcgttg ttgtcgctgg cgctattacg gcgcggctgg caggtaacgc tttattgcgc
    35401 ggatgaggcc cccgcactgg gtgcttccgg caatcgccag ggggcgctgt atccgttatt
    35461 aagcaaacac gatgaggcgc taaaccgctt tttctctaat gcgtttactt ttgctcgtcg
    35521 gttttacgac caattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg
    35581 ctgggatgag aaaagccagc ataaaatcgc acagatgttg tcaatggatt tacccgcaga
    35641 actggctgta gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg
    35701 cagcggcatt acttatccgc aaggtggttg gctgtgccca gcagaactga cccgtaatgt
    35761 gctggaactg gcgcaacagc agggtttgca gatttattat caatatcagt tacagaattt
    35821 atcccgtaag gatgactgtt ggttgttgaa ttttgcagga gatcagcaag caacacacag
    35881 cgtagtggta ctggcgaacg ggcatcaaat cagccgattc agccaaacgt cgactctccc
    35941 ggtgtattcg gttgccgggc aggtcagcca tattccgaca acgccggaac tggcagagct
    36001 gaagcaggtg ctgtgctatg acggttatct cacgccacaa aatccggcga atcaacatca
    36061 ttgtattggt gccagttatc atcgcggcag cgaagatacg gcgtacagtg aggacgatca
    36121 gcagcagaat cgccagcggt tgattgattg tttcccgcag gcacagtggg caaaagaggt
    36181 tgatgtcagt gataaagagg cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc
    36241 aatggtaggc aatgttcccg attatgaggc aacactcgtg gaatatgcgt cgttggcgga
    36301 gcagaaagat gaggcggtaa gcgcgccggt ttttgacgat ctctttatgt ttgcggcttt
    36361 aggttctcgc ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cgcagatgag
    36421 cgacgaaccg attccgatgg atgccagtac gctggcggcg ttaaacccga atcggttatg
    36481 ggtgcggaaa ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tcgcagtgtt
    36541 tgccggatgc ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc ctgataagac
    36601 gcgataagcg tcgcatcagg catggtgctc cagacgccgc aacttactgc tgtgacgcct
    36661 gctgaaacag gttttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac
    36721 cggcaccaat ggctttttcc agactctggc taacggtcgt atgcaccgcc tgagcggagt
    36781 ggtcatcacc actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac
    36841 tggcaaacaa ctcatcatca ctggcatggt caaccatacc gtcgattaat gccagaatgc
    36901 gtgattcaaa ctccgcgatc atcttctttc ctcattgtaa aacgtggcgc tggctttagt
    36961 tgagcgcttc cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt
    37021 gtagtgcttt aatgaagcgt gccgggcgtt caggaatgcc gtttgctagg taatccatca
    37081 cctgcgcatg tacccgccgt tgaaacacca cacgatccgg ttcgaagtcg ccttccagat
    37141 tgtcgcaact gacgttaaac ggatatcccg ccgccacaca gaacaaccag tcgagtgcct
    37201 gcggcttcac ttcaacatct tcaaactggc tttgcgtttg ggcatcgcgt ccgtccgggc
    37261 aataccagta gccgaaatca accagctcac ggcgcgcttt tccggcaata caccagtgcg
    37321 aaatctcatg aattgcgctg gcataaaagc cgtgggcaaa gacaattcgg ttatacggta
    37381 cttccgcatc agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa
    37441 attcatcggc aaagcagcta ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt
    37501 tcattagttc atccccaacc agtggaggat ctcctgtccg tggctatcgt aaagtaattt
    37561 ggcactcatc accgccgaga cgataacaat catcgggcgg atcagctttt gtcctttgct
    37621 caacaccagc cgtgaaccca tgcgcgcgcc gaggaactgc cccaccagca tcacaaagcc
    37681 cgtcgcccaa atcactttgc cgccgagaat aaacagcagc agaccgccaa tgtttgacgt
    37741 tgcgttgagt aatttggcgt gagccgtggc tttggcgagg ttgaatccgc acagcgtaac
    37801 gaaggccagc gcgtaaaacg atccggcagc cgggccaaag aatccgtcat aaaaaccgac
    37861 gcagccaccg gcaattaacg cgaagggtaa accgtacatc cggcgctggc gatcttcttc
    37921 acccagcttt ggcatcagca agaaatagag gccgatacaa atcaccagaa tgggcaaaat
    37981 ctgccgtaag acatcagcct gaacgtactg taccaacagt gcgccgctca ttgagccgac
    38041 aaaggtcatg gcaatattga gtttctgatc gcttaaacta accactttgc ggcgaataaa
    38101 gtagatagtg gcggaaatag agccgccgca ggcttgcagt ttattggttg ccaacgcgtt
    38161 agcgggagac attcccgctg ccatcaatgc cggaatggtg agtaacccac cgccaccggc
    38221 aatcgagtcg ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg
    38281 ggaaaccata aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc
    38341 tggcaggaag gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt
    38401 ggaggtgcaa accagctttg cagttctgcc ccgcaaccat cgcctggtgg cggtaaaggt
    38461 tgatcttcac actccagact atcggcagga caacgtaatc gtacatgcat atgcgcgcga
    38521 tgctggaacc agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atcaaggcaa
    38581 agttgttgtt taatcgccgg attaacaaaa atgcgtgtga cgtctttgtc ctgggcggcg
    38641 agtttgatca agctgaaaat ttctggcttc cacagcgtcg gaacaacgtg tttaccgtcg
    38701 cgggaaacta agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca gcgagttttc
    38761 ggcagttgca gaaagatatc gacatccagt ccggtctggt ggctggcatg accgccgttg
    38821 aaacgcccac cagcgggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg
    38881 ctcacctggc tactcagacg ctggataaac atcaccagat ccgggtgacc gaaatagcga
    38941 cgctgatcgg tacgcatgac ctgataatgt tcggactgta tcggcaacgt gtcagcgccg
    39001 acaatacagc cattagaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt
    39061 attttttgcc acggcgtcgc tgccaggcta gcgctactgg caagcagagc cagcagcgca
    39121 atcgcggttt tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc
    39181 gcccgctgcc gtaacaggtg atccattaaa acgatcgcca gcatcgcttc tgcgatcggc
    39241 actgcacgga tcccgacaca gggatcgtga cggcctttgg tgatcatctc aacttcttcg
    39301 ccaaagcggt taatagtgcg ccccggcacg gtaatgctgg aggtcggttt cagcgccata
    39361 tgggcaatga tttgctgccc gctgctgata ccgccgagaa tgccgcccgc atggttgctc
    39421 tggaaaccgt ctttggtgat ttcgtcgcgg ttctggctgc cgcgcagcgc cactacgtca
    39481 aaaccgtcgc caatttccac gcctttcacc gcgttgatgc tcatcagcgc atgggcgatg
    39541 tcggcatcca ggcggtcaaa gaccggctcg ccaagtccgg cggggacacc actggcaaca
    39601 acggtgactt tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat cagttcatct
    39661 aacgcgtcga ttttgtcggg gtccgggcag aagaacggat tttgttcaac cagcgaccag
    39721 tctttgattt ccagcggaat gtcgcccatc tgggtcaggc agccgcggat ttcaatacca
    39781 aatttctcgg cgagatattt tttggcaatc gcccctgccg ccacgcgcat ggcggtttcg
    39841 cgggcggaag aacgtccacc gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg
    39901 taatcagcat ggcctggacg gaagacgtct ttaattgcgc tgtaatcctg agaacgctgg
    39961 tcggtatttt cgatcaacaa gccaatgctg gtgccagtag taacgccttc aaaaacaccg
    40021 gagagtattt tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga tgtcccaggg
    40081 cgacgacgat caaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca
    40141 ccatcgacga tgcagccgag cgccagcccg tgcgattcgc cgaaggtggt tacgcgaaag
    40201 agttgtccaa ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac
    40261 ttaatcttta taaatcgcga aatgttctcg tgcggcaata agctgctctt tggtgagcat
    40321 aaacactcca tcgccgccgt tatcaaactc cagccaggtg aacggaacat cgggatattg
    40381 ttccataaga tgtaccatgc tgttgcccac ttcacaaatc aacacgccat catccgcaag
    40441 gtaatctgcc gcgttaccga gaatgcgacg cgtcagtttc aggccgtcgg tgccagatgc
    40501 caggcccagt tccggctcgt gacggtattc gtttggcagg tcggacatat cttccgcgtc
    40561 gacatacggc gggttagtga caatcagatc gtattgcact ttcggcaaat cacggaacag
    40621 atcggaacga atcggaatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc
    40681 aaccgccagc gcgtcgggag agatatctac cgcgtcgact tccgcttccg ggaaggcata
    40741 agcacaggca atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg
    40801 cttgctgatg agtccggcaa atttattgtt gatcagttca ccaatcggtg agcgcggcac
    40861 cagcacgcgt tcatcgacgt aaaattcatg accgcagaac caggctttgt tggtcaggta
    40921 agccaccgga atgcgctcgt tgacgcggcg gatcacgcgt tcaacaatac ggtgtttttc
    40981 gctggaggtc agacgcgcgg tgcgcatatc ttccgggata tccagcggca ggtagagcga
    41041 tggtaacacc agctgtacgg cttcatccca cgggttatcg gtgccgtgac cgtaccagat
    41101 atttgccgcg ctgaagcggc ttaccgacca gcgcaacatg tcctgaatgg tttgcagctc
    41161 atttactgct tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata
    41221 atttcggcgg ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg
    41281 aggaaaaata cgtttaaaac gatcgattgt gctacgagtc gggtaaactg taggaaaatt
    41341 agaaatagag acagataaat gaaaaagaaa acaacactta gcgaggagga ccaggctctg
    41401 tttcgccagt tgatggcggg gactcgcaag attaagcagg acacgattgt ccaccgaccg
    41461 cagcgtaaaa aaatcagcga agtgccggtg aaacgcttga tccaggagca ggctgatgcc
    41521 agccattatt tctccgatga gtttcagccg ttattaaata ccgaaggtcc ggtgaaatat
    41581 gttcgcccgg atgtcagcca ttttgaggcg aagaaactgc gccgcggcga ttattcaccg
    41641 gaattgtttt tggatttaca cggtctgacg cagctgcagg ccaagcagga actgggcgcg
    41701 ttgattgccg cctgtcgccg cgaacatgtg ttttgtgcct gcgtgatgca tgggcacggg
    41761 aagcatattt tgaagcaaca aacaccgctg tggctggcac aacatccgca tgtgatggca
    41821 tttcaccagg caccgaaaga gtacggcggt gatgcggctt tgttggtgtt gattgaagtg
    41881 gaagagtggt tgccgccgga gttgccctga ggagttgagc gtgttgtgcc aggagcgcgg
    41941 aatggcaggg gcgaatgacc ctgccaggtg agaaaaggtg cgctaataca tattcagtat
    42001 gtaagtgaat cggcaggtag aatctatggg ctgtcgagat gtcgtgtcca cgcgtagctt
    42061 catatcatgc agttgcctgg acgtaatcgg ttgcggctca ggcgtcaccg tcactttggc
    42121 attatcacag ccggaagtcg agaaggtcat ctgctgtgcg ataaaacggc agtaggcacc
    42181 acgctcatca aaattattcc cgttacacag tttgccattt tccatcagga aagtgggcag
    42241 tgtttgctca ataacaccac ctgttgactg caacctgacg actttgttat gtacaacata
    42301 attttcattc caggaaacat catacggtgc caggggcacc gccggacatg aagaactcac
    42361 cttcgttggg ccaatatagt caggaaaatc atcatcatac gttttagcga agggatctct
    42421 actcttaaca aacgacgaca caatccacgg acaaccatgt aaattcccat cgtaataaac
    42481 aaacatcttt aattcattac atgtccatcc cgttttggtc tggctatcat actgctctaa
    42541 tccaggacag gctgacatgg ataccctgct gtccgaaatt ttcgacatag atggttgagt
    42601 agaactatcg ccagtccacg aaactaaatc cggctgtgaa ttgggcatga atgtagcctt
    42661 aaatatagcg taattaactg aatctggcgt cgctataatc agatgaaact caaactctgt
    42721 cgcaggtgaa ctggcaccag ctggcacata taactcacca actttcgtcg ccgcctggcc
    42781 gcatgtattg aacattaaca gcaataaaag acataaaaca tgacgaagga ttttcatttt
    42841 aaatatactc aaaagttgct gtcaggctgg cggtgaaatc accgatggtt acatcacgac
    42901 cgcttttcgc ctggatccag gcccggaaat taatatcatt gttgccgttg gtaagcgcaa
    42961 aggtcgcccc ggtggtgttg ttaatatcca cccgcttgcc gtcgagcgtt tccatcccta
    43021 tccccacgcc ctgcgcggtg gaggtgtcat cgactgccag aaaacccggc tgaccgctgt
    43081 cttcggtgcc gctcagcgtg actttgacgt tgtatcccgc cggccctttg cagtctttca
    43141 gcttaaacac aaccggcaga tgcgcggact gcccagatac catcaaatcc cggttgctga
    43201 cggtgggaaa atggatttct gccaggttgg caccatccac caccagcgtg caggatttac
    43261 tcagcaggtt gccgtaaaaa tgcaggttgt tgtcgactgc ctgagctcct gtgctggcgg
    43321 tcagcagcag gcctgctaac agcgcataac cgggtgaata aatgtgtgct gttttcattg
    43381 gtaatccacc cgtaaggttg cgtaagcaga aaaggtcgtg tccgtcagca ccgcatcagc
    43441 cgctttgacc ggtaccgcct ccagctttgg cttctggcta aaatcggtgg cgttaatgga
    43501 aagcggctcg ttgagcttaa aacgctgacc gtcatgctga agctcaattc cgaagcccgg
    43561 tacatccgtc tcaatagcag agtcgttgta ggaggtggca gtccccgtcc aggagagtgt
    43621 catattccac tcgggatcgc ggacatccga gtcgcagctc agttcgtagt ccactttctg
    43681 acgaccataa ttgccatcaa tgctgtcgat aatgagatcg cgaaattgca cttcgatggt
    43741 ttgcccgccg ctgatgctac aggcgggcgg cgacatcagc gtaccgtgga atgtgatgtc
    43801 atcttccgcc gccagcacag ccccgcaacc agcgcacagt aaaaggagca ggaatttttt
    43861 cattggtaat ccaccaccat ggtcatcgtg gcgttaaact ccgacggcgt cagttgcacc
    43921 ccgctctgtt ttaccggcac cgctttcagc tgaggctgag tgttgacatt aaaaggcgtc
    43981 cagttatttt caccaactgc aaacagggag ttgtcggcgg cgttttctat ccgcaccccc
    44041 aggccggcga tatccgtgct gagaaccgtt tctccgttga tcacggcggt attgcctttg
    44101 agctgcatcc gtaagtcatc gctgacttta ttaccgcagt tgagggtgta tttcgcatcc
    44161 tgttggtaat tggtaccatc gatcctgttg attatcacat tgccaaattt cacagcgctt
    44221 cccttcacgg agcaaggcgg cggtccatcg accaggattc gcagcgtcag atccacctgt
    44281 ttgatatcac tgccagcaac agccaaaccg ctgatgcccc atccggccag caccagcaac
    44341 cctgcgcccg ctacaaactt gtgactcata agtccctccc ggcatcagcc tttgcggctc
    44401 tgctcttcat ccacctggca gctgttgccg gtgcatatga agtacagcgg catccgcgcg
    44461 ccgtagtcgt tgatgtaagt cagcaccggc acgctgtcca tcttcgcatt cagcggtgcg
    44521 gaggttttcg gctccatcat cagcggtgtg aatcccgccg gcgggttgcc gcctttttta
    44581 gcgctggcgt tgctgatgat cacgtagtac ggcgtcgggt tattgacggt gaagtcctgg
    44641 ccgttacgga tgagcgtcac cttatgctgc cacggacttt tcatgctggt gttctccagc
    44701 gccttcggtc gccagaacag tttgatacgc gtctgcagcg caatctgcag tgtgttcggt
    44761 ttatttgatt tcggcggaat ttcgcgcacg ttgaaataga acacgctctc gcggtcggcc
    44821 ggcagtttgt tgatatccgg cagcccctgc accttcacct ggccgttcat catcgcatcg
    44881 atgcgctgca ctggcggaag caccgcaagc ggcgagctga ttttgttgcc ctttgcatcc
    44941 tctatccagc tctgcgccag ataaggcagt gtgggatcgt tgttgcgcag cgtgacgctg
    45001 atggacttgt cgctttcgtt aaacaccagc cgtgtcctgt cgggcgtcac cgaagcgtgg
    45061 ctggagaaac tgaccgctgt cgccagcagc gccagtgccg ggacaacgat ttttgcagtg
    45121 ttagtcagat taaacatgga tcattcctta atcacgttac tggtttactg aaattgaagt
    45181 cggaggttcc gttggcgtca cccgctgtgt ctgttcctga atcgccggtt tgaccgccgg
    45241 cgaagtgata ggtgctacat tgcctttatg ctggcatggc agtaacaagc cgttaaacag
    45301 atcggcaggc agcgggtccg gcaggttgat atcgcaatgc gcaacaccac tccagaacac
    45361 actcatgtgt tcgccaggtt tcaccccagc cagataaaca ttgccgtcat catcgacaag
    45421 gcccactgtc tgctcgttat cattttttac ttctgcgcca aacggaggat ggctgccgtc
    45481 ttgtaaacgc agcaccgcca tcgctttttg accactgatg acggcaaatt tgcggtagcc
    45541 aatcgcccct tcagttagcg tggcttgtac caccgactgg gttgcttcgg cgttttccgg
    45601 cagtttgttg aggtcgatat acgcctgatt gcggtaatag ttattgacgt cagaaacgac
    45661 ggctttacca aacatattgg tataaacagc agcaccgtta ccttctaccg gaacatcggc
    45721 aacgccatcg gcatcaatca acaagcgtgt cccgcccata ttctgggtac ggtgaagtgc
    45781 gccaccgtgg gcagtaagcg ttgcgccgcc ctgtaacgac aagcccgcag aggtgtattg
    45841 cccttcatgg tagttcgcac tgaggtcaac ctgcgccagc gaaccatcat ggctgtaata
    45901 gccgtcaacg ctggtgtgtt tgtcactggt gccgacgttc aactgatagt gagtcgcgtc
    45961 atcgacacgg ctgaaataac cgacctgact gctgtccgtc ccactgccat agttaccgtt
    46021 atagctaacg gtactgttgt cgccccacgg catactgagc gaaatgtaca tgcctttgtc
    46081 ggcctggttg tcatactcgt aacggtagcc agtcatcgag atgctgacat tacgaatact
    46141 gcccatattg aaatagtgcg agagcatgat gttgtagttt gtctgctcct cgcgatccca
    46201 gtaggtatgg cgggtatagt tgagataaac cgaaacaccc gcatcgcgga agttctggtt
    46261 ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt accattccgc tgtcactggc
    46321 atccagatac tcgctcatgg tcataaagtt ctcttccgag aagcgatatc cagcaaaagt
    46381 aacgcggctg ttgagctggt caaagtcttt ggaataactc acacggaagg agttaccgtc
    46441 cagcgaacct ttgccataag cggtgtcttt atccagtttg gtgtgcgagt gagtaacatc
    46501 aaacgcgacc gcgccgaatg tagacaaatc gcgaccgacg ccaagcgccg cagactgata
    46561 gttttcatct cccagtgcgc cgccatataa cgaccagccg ttagcgatcc cccaggaagc
    46621 ttcagcacca gaaaaaaatt caccctcgac atggtgtccc cactcttgcg gacggcccat
    46681 catgatctta tagcgaacct gacctgggcg agtgaggtat ggcatcgagg cggtgctgat
    46741 gtcatattcc tgcacctggc cgttctgttc ttcaatgcga atatgcaacg taccggagac
    46801 agaatcacca agatcctgaa tacgaaacgg tccagccgga acctgcgttt cgtaaatcac
    46861 acgccccatc tggctgacgg tcacttttgc tgtggtgtgt gccacgccgg aaatgtctgg
    46921 cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca gtactgacac tgccaccaac
    46981 atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc tcgcccagcg ccagtttggc
    47041 ttttaatgat ggtaacgccc gccaggcata gtagcgactc cactcccatt ttttttgagt
    47101 ttcatcgccg ctgaattcat cgtcatcatt actgcgagta tgttgatagt tagtctgcca
    47161 gtcagcacgc atacgccacg gccccaggtt aaccccgacc gtcccgttgc cgctgatctc
    47221 gttactatca tcaccgccat tttcttcgtg ccgggtttgt gcgttgatgc tgtagtccgc
    47281 gacgatcccg gagatgccgt catcccaacg tgaaggcgga tcccagtcgg gataagtata
    47341 ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac tggcttaaat cagccttaat
    47401 ttccatgcct tccagttgac cggatttcag gcatttagca tcgtggctcc attgcagatt
    47461 tttcgccacg tcttctttta aaccaaactg cgctaccagt tccggtgtca gacaagcata
    47521 gcttttgctc gcgtcatctt caccagcata ccagtaaata tcgtactctt ccgccaatgg
    47581 ctgtttattt agttgaacct gtaaattgta ttttccgggc tcaacatatc cctggctgga
    47641 aaaacgcttc agatcaattt ttgtgtcgcc ttttaattcc agaaaacggg aatcaaactg
    47701 aatgtcgtct tcagcccaga cactgctata actccctgac attgccagcg caatgcacca
    47761 cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata cacgtttcta tccgttttta
    47821 ttcttgttcg gtcaaaatcc atttaaatcc cccatagcag tgtcgctatg ggggtatgcc
    47881 ttacaggtaa gtaatctgga aggtggtatt tgcttcgaaa ttacccagat ctggcgcatc
    47941 agctgcgccg accagccagg ccttaaagtt cagtgtctgc ttcgctttgc ctttattggt
    48001 agaagtgtct ttcactattt tctggtcaat acccatgccg cttttgtaag aggtacccag
    48061 agagtcaccg atcgccaggc taacattgtt aaatgccgca ccggtatcgg tattgaaaat
    48121 ggtgtaataa ttgccgctat ttgcagaaga aacggtacca gtaaaggtag tggtcattgt
    48181 ttcctgagta tcaaatacgc aatcttgcag acgaatctgg aaacttttcg gcgtggtggt
    48241 gccgttattt ttcagttttt cggcaccgat atcacccata tcaacttcca gtttgtgatc
    48301 gtccggaacg atagaacaca ctgagtcttc aatggtgccg taaaaacgtg cagtaccgtt
    48361 gttacccgca gcgattgttg ccgtagaggt gaaaacgccc atcaccattg ccgcagcaat
    48421 agctgtttta acaaacttac tcattatttg tccttaaact ataaataatt aaaaatataa
    48481 acattacaat a
//
LOCUS       DAPGWD010000018        48343 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPGWD010000018 DAPGWD010000000
VERSION     DAPGWD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.018"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..4498
                     /gene="sslE"
                     /locus_tag="RHX88_000972"
     CDS             <1..4498
                     /gene="sslE"
                     /locus_tag="RHX88_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HDV2229698.1"
                     /translation="DGGGSGSSSDTPPVDSGTGSLPEVKPDPTPNPEPTPEPTPDPEP
                     TPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDESSDGFTFTPGDKVTC
                     VAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKSNALSLVTSMNSCP
                     ANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATDKAPSTHTSPV
                     VPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSEGRLVDSQGYGVAGVNYYTNSGR
                     GVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANIDQLIHRYS
                     TTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLDEGEQVVNLPNEFIEQFKT
                     GQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTNYKSVSKFHVF
                     HDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWDKNELAYITEA
                     PSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPHYNSILRCPNGYSWNGGVNKD
                     GQCTLNSDPDDMKNFMENVLRYLSDDKWTPDAKASMTVGTNLDTVYFKRHGQVTGNSA
                     AFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGNDPYAIPLRADTSKPK
                     LTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSMALNKSVVNND
                     PQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTGEVKWKYQVENKPDDKPKLEV
                     ASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKEKIFAAFPGLKECTNPAYHYEVNCL
                     EYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADLGTNIQRLYQHELYFRTNGRK
                     GERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGFKTFTEFLNCYANDAYAGGTK
                     CSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYMEKPLTRLMLGRSWWDLNIKVDVE
                     KYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTIKSNANVPVTVTV
                     ALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYIKGNSSTNESASF
                     TFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTTPKKNLNASNYTGGLKQFAND
                     LDTFASSMNDFYGRDETSGKHRMFTYKALTGHKHRFTNDVQISIGDAHSGYPVMNSSF
                     STNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQDRYLGKMNRV
                     ADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAEKNFDIKKWYPDGTPLPEFY
                     SEREGMKGWNLFQLMHRKARGDDVGNKTFGGKNYCAESNGNAADTLMLCASWVAQTDL
                     SAFFKKWNPGANAYQLPGATEMSFEGGVSQSAYSTLASLKLPKPEQGPETINKVTEHK
                     MSLE"
     gene            4628..5437
                     /gene="pppA"
                     /locus_tag="RHX88_000973"
     CDS             4628..5437
                     /gene="pppA"
                     /locus_tag="RHX88_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HDV2229699.1"
                     /translation="MLFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMSSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATIYLQALF"
     gene            5503..5913
                     /gene="gspS2"
                     /locus_tag="RHX88_000974"
     CDS             5503..5913
                     /gene="gspS2"
                     /locus_tag="RHX88_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HDV2229700.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            5931..6890
                     /gene="gspC"
                     /locus_tag="RHX88_000975"
     CDS             5931..6890
                     /gene="gspC"
                     /locus_tag="RHX88_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDV2229701.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTNKKAVSDEAKQAVAEPAASAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            6920..8980
                     /gene="gspD"
                     /locus_tag="RHX88_000976"
     CDS             6920..8980
                     /gene="gspD"
                     /locus_tag="RHX88_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDV2229702.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            8980..10473
                     /gene="gspE"
                     /locus_tag="RHX88_000977"
     CDS             8980..10473
                     /gene="gspE"
                     /locus_tag="RHX88_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDV2229703.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            10473..11696
                     /gene="gspF"
                     /locus_tag="RHX88_000978"
     CDS             10473..11696
                     /gene="gspF"
                     /locus_tag="RHX88_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDV2229704.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDQTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            11713..12168
                     /gene="gspG"
                     /locus_tag="RHX88_000979"
     CDS             11713..12168
                     /gene="gspG"
                     /locus_tag="RHX88_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDV2229705.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            12172..12735
                     /gene="gspH"
                     /locus_tag="RHX88_000980"
     CDS             12172..12735
                     /gene="gspH"
                     /locus_tag="RHX88_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDV2229706.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            12732..13103
                     /gene="gspI"
                     /locus_tag="RHX88_000981"
     CDS             12732..13103
                     /gene="gspI"
                     /locus_tag="RHX88_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDV2229707.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            13100..13699
                     /gene="gspJ"
                     /locus_tag="RHX88_000982"
     CDS             13100..13699
                     /gene="gspJ"
                     /locus_tag="RHX88_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDV2229708.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            13702..14679
                     /gene="gspK"
                     /locus_tag="RHX88_000983"
     CDS             13702..14679
                     /gene="gspK"
                     /locus_tag="RHX88_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDV2229709.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLEQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            14676..15854
                     /gene="gspL"
                     /locus_tag="RHX88_000984"
     CDS             14676..15854
                     /gene="gspL"
                     /locus_tag="RHX88_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDV2229710.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            15856..16392
                     /gene="yghD"
                     /locus_tag="RHX88_000985"
     CDS             15856..16392
                     /gene="yghD"
                     /locus_tag="RHX88_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDV2229711.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(16541..16616)
                     /locus_tag="RHX88_000986"
     tRNA            complement(16541..16616)
                     /locus_tag="RHX88_000986"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16581..16583),aa:Phe,seq:gaa)
     gene            complement(16722..17429)
                     /locus_tag="RHX88_000987"
     CDS             complement(16722..17429)
                     /locus_tag="RHX88_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDV2229712.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            17827..19962
                     /gene="speC"
                     /locus_tag="RHX88_000988"
     CDS             17827..19962
                     /gene="speC"
                     /locus_tag="RHX88_000988"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV2229713.1"
                     /translation="MKSMNIAASSDLVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVEMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(20012..21268)
                     /gene="nupG"
                     /locus_tag="RHX88_000989"
     CDS             complement(20012..21268)
                     /gene="nupG"
                     /locus_tag="RHX88_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDV2229714.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(21470..22549)
                     /gene="mltC"
                     /locus_tag="RHX88_000990"
     CDS             complement(21470..22549)
                     /gene="mltC"
                     /locus_tag="RHX88_000990"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDV2229715.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(22614..22889)
                     /locus_tag="RHX88_000991"
     CDS             complement(22614..22889)
                     /locus_tag="RHX88_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDV2229716.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(22917..23969)
                     /gene="mutY"
                     /locus_tag="RHX88_000992"
     CDS             complement(22917..23969)
                     /gene="mutY"
                     /locus_tag="RHX88_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDV2229717.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            24130..24849
                     /gene="trmB"
                     /locus_tag="RHX88_000993"
     CDS             24130..24849
                     /gene="trmB"
                     /locus_tag="RHX88_000993"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDV2229718.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            24849..25175
                     /locus_tag="RHX88_000994"
     CDS             24849..25175
                     /locus_tag="RHX88_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDV2229719.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            25359..26078
                     /locus_tag="RHX88_000995"
     CDS             25359..26078
                     /locus_tag="RHX88_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDV2229720.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKYFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            26254..27300
                     /gene="ansB"
                     /locus_tag="RHX88_000996"
     CDS             26254..27300
                     /gene="ansB"
                     /locus_tag="RHX88_000996"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDV2229721.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            27417..28424
                     /locus_tag="RHX88_000997"
     CDS             27417..28424
                     /locus_tag="RHX88_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDV2229722.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSGINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(28579..29715)
                     /gene="hemW"
                     /locus_tag="RHX88_000998"
     CDS             complement(28579..29715)
                     /gene="hemW"
                     /locus_tag="RHX88_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDV2229723.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(29708..30301)
                     /locus_tag="RHX88_000999"
     CDS             complement(29708..30301)
                     /locus_tag="RHX88_000999"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDV2229724.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(30309..30599)
                     /locus_tag="RHX88_001000"
     CDS             complement(30309..30599)
                     /locus_tag="RHX88_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDV2229725.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(30596..31162)
                     /gene="yggT"
                     /locus_tag="RHX88_001001"
     CDS             complement(30596..31162)
                     /gene="yggT"
                     /locus_tag="RHX88_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDV2229726.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(31180..31884)
                     /gene="yggS"
                     /locus_tag="RHX88_001002"
     CDS             complement(31180..31884)
                     /gene="yggS"
                     /locus_tag="RHX88_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDV2229727.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            31902..32882
                     /locus_tag="RHX88_001003"
     CDS             31902..32882
                     /locus_tag="RHX88_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDV2229728.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(33058..33474)
                     /gene="ruvX"
                     /locus_tag="RHX88_001004"
     CDS             complement(33058..33474)
                     /gene="ruvX"
                     /locus_tag="RHX88_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDV2229729.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(33474..34037)
                     /locus_tag="RHX88_001005"
     CDS             complement(33474..34037)
                     /locus_tag="RHX88_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDV2229730.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(34146..35096)
                     /gene="gshB"
                     /locus_tag="RHX88_001006"
     CDS             complement(34146..35096)
                     /gene="gshB"
                     /locus_tag="RHX88_001006"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDV2229731.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(35109..35840)
                     /gene="rsmE"
                     /locus_tag="RHX88_001007"
     CDS             complement(35109..35840)
                     /gene="rsmE"
                     /locus_tag="RHX88_001007"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDV2229732.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(35920..36627)
                     /gene="endA"
                     /locus_tag="RHX88_001008"
     CDS             complement(35920..36627)
                     /gene="endA"
                     /locus_tag="RHX88_001008"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDV2229733.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAAGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(36722..37219)
                     /locus_tag="RHX88_001009"
     CDS             complement(36722..37219)
                     /locus_tag="RHX88_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDV2229734.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(37296..38690)
                     /gene="galP"
                     /locus_tag="RHX88_001010"
     CDS             complement(37296..38690)
                     /gene="galP"
                     /locus_tag="RHX88_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDV2229735.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(39126..40280)
                     /gene="metK"
                     /locus_tag="RHX88_001011"
     CDS             complement(39126..40280)
                     /gene="metK"
                     /locus_tag="RHX88_001011"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDV2229736.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(40584..40799)
                     /locus_tag="RHX88_001012"
     CDS             complement(40584..40799)
                     /locus_tag="RHX88_001012"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229737.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            40935..41066
                     /gene="yqgB"
                     /locus_tag="RHX88_001013"
     CDS             40935..41066
                     /gene="yqgB"
                     /locus_tag="RHX88_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDV2229738.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            41075..43051
                     /gene="speA"
                     /locus_tag="RHX88_001014"
     CDS             41075..43051
                     /gene="speA"
                     /locus_tag="RHX88_001014"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDV2229739.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            43197..43928
                     /locus_tag="RHX88_001015"
     CDS             43197..43928
                     /locus_tag="RHX88_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229740.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            44064..44984
                     /gene="speB"
                     /locus_tag="RHX88_001016"
     CDS             44064..44984
                     /gene="speB"
                     /locus_tag="RHX88_001016"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDV2229741.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(45190..45948)
                     /gene="loiP"
                     /locus_tag="RHX88_001017"
                     /gene_synonym="yggG"
     CDS             complement(45190..45948)
                     /gene="loiP"
                     /locus_tag="RHX88_001017"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDV2229742.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            46226..48217
                     /gene="tkt"
                     /locus_tag="RHX88_001018"
     CDS             46226..48217
                     /gene="tkt"
                     /locus_tag="RHX88_001018"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV2229743.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
BASE COUNT        11689 a        12247 c        13150 g        11257 t
ORIGIN      
        1 tgatggcggt ggctccggat cttcctccga tacgccgcct gtagattctg gaacagggtc
       61 tttgccggaa gtgaaacctg atccaacacc aaacccggag ccgacgcctg agccaacgcc
      121 ggacccagaa cctacgccgg aaccgacacc tgatcctgag ccaacaccag aaccggagcc
      181 agaacctgtt cctacgaaaa cgggttatct gaccctgggc ggaagcctgc gggtaactgg
      241 tgatatcacc tgtaatgatg aatccagcga tggctttacc tttacaccag gcgacaaagt
      301 cacctgtgtg gcagggaaca acacgacaat tgctaccttc gacacccagt cagaagctgc
      361 gcgtagcctg cgtgcggttg aaaaagtgtc gtttagtctt gaggacgcgc aagaactggc
      421 gggttccgac aacaagaaaa gcaatgcgct ctcgctggtc acctccatga acagttgccc
      481 ggcgaataca gaacaggtgt gcctggagtt ctcctcggtg atcgagagta aacgtttcga
      541 ctcgctgtat aagcaaatcg atctggcacc ggaagagttc aaaaagctgg tcaatgaaga
      601 ggtggaaaac aatgccgcga ctgataaagc gccatccact catacttcac cggtagtccc
      661 cgtcacgact ccgggaacaa aaccggatct aaacgcctcc ttcgtgtcgg ctaacgcgga
      721 acagttttat cagtatcaac cttcggaaat cattctctca gaaggtcgcc tggtggatag
      781 ccagggatat ggtgttgctg gcgtcaacta ctacaccaat tcaggccgtg gcgtgacagg
      841 ggaaaatggt gaattttctt ttagctgggg cgaaaccatc tcctttggta tcgatacctt
      901 tgaactgggt tcagtgcgcg gcaataagtc gaccattgcg ctgactgaac tgggtgatga
      961 agttcgcggg gcgaatatcg atcagcttat tcatcgctat tcgacgaccg ggcaaaataa
     1021 tacccgtgtt gttccggatg atgtacgcaa ggtctttgcc gaatatccca acgtgattaa
     1081 cgagattatc aatctctcgt tatccaacgg tgcgacgctg gatgaaggtg agcaagttgt
     1141 taatctgcca aacgaattta tcgagcagtt taagacgggt caggccaaag agatcgatac
     1201 cgcgatttgt gcgaaaaccg acggttgtaa cgaggctcgc tggttctcgc tgacgacgcg
     1261 caatgttaat gacggccaga ttcagggcgt tatcaacaag ctgtggggcg tggatacgaa
     1321 ctacaaatct gtcagcaagt tccatgtatt ccatgactct actaacttct atggcagcac
     1381 gggtaatgcg cgcggtcagg cggtggtgaa tatctccaac gcggccttcc cgattctgat
     1441 ggcgcgtaat gataaaaact actggctggc gtttggcgaa aaacgcgcct gggataaaaa
     1501 cgagctggcg tacattacgg aagcgccttc tcttgttgag ccggaaaacg ttacgcgcga
     1561 taccgccact ttcaacctgc cgtttatttc gctggggcaa gtcggtgagg gcaaactgat
     1621 ggttatcgga aacccacact acaacagcat tttgcgttgc ccgaacggtt acagctggaa
     1681 cgggggcgtt aataaagatg ggcagtgtac gctcaacagc gacccggatg acatgaagaa
     1741 cttcatggag aacgtgctgc gctatctgtc cgacgataaa tggacgccgg acgcgaaagc
     1801 cagcatgacc gtaggcacca acctagatac tgtctatttt aaacgtcatg gtcaggttac
     1861 aggaaacagc gctgcgttcg acttccatcc ggattttgcg ggcatctctg ttgagcattt
     1921 aagtagctat ggcgatctcg atccgcagga aatgccgctg ctgatcctta acggctttga
     1981 atatgtgact caggtgggta acgatcctta tgcaatcccg ctgcgtgcag ataccagcaa
     2041 accgaagctg actcagcagg atgtgaccga tctgatcgcc tatctgaaca aaggtggatc
     2101 ggtgctgatc atggaaaacg tgatgagcaa tcttaaggaa gagagcgcgt ctggttttgt
     2161 gcgtctgttg gatgccgcag gtctgtcgat ggcactgaac aagtcggtag taaataacga
     2221 tccgcaaggg tatccgaacc gcgttcgtca gcagcgcgca acgggcattt gggtctatga
     2281 acgttatcct gccgtagatg gtgcgctgcc gtacaccatc gatagtaaga caggggaagt
     2341 taagtggaaa tatcaggtag aaaacaaacc tgatgacaaa ccgaagctgg aagttgccag
     2401 ctggctggaa gatgtagatg gcaaacagga aacgcgttat gcctttattg atgaggccga
     2461 tcataaaaca gaggattctc tgaaggctgc gaaggagaaa attttcgccg cgttcccggg
     2521 gctgaaagag tgtactaatc cggcatatca ctatgaggtc aactgcctgg aatatcgtcc
     2581 tggcacgggg gttccggtta ctggtggcat gtatgttcca cagtatacgc aactaagcct
     2641 taacgccgac actgcaaaag cgatggtgca ggctgcggat ttaggcacca acattcagcg
     2701 tctgtatcag catgagctct acttccggac caatggtcgc aaaggtgagc gtctgagcag
     2761 cgtcgatctg gaacgtctgt accagaacat gtcggtctgg ctgtggaacg atacgagcta
     2821 tcgttatgaa gaaggcaaaa atgacgagct gggctttaaa acgttcaccg agttcctgaa
     2881 ctgctacgcc aatgatgcct atgcaggcgg caccaagtgt tctgcagatc tgaaaaaatc
     2941 gctggtcgat aacaacatga tctacggtga cggtagcagc aaagcgggca tgatgaaccc
     3001 gagctacccg ctcaactata tggaaaaacc gctgacacgc ctgatgctgg gccgttcctg
     3061 gtgggatctg aacatcaaag ttgatgtcga gaagtatccg ggagcagtat cggaagaggg
     3121 acagaacgtt actgaaacca tcagcctgta ctcgaatccg accaaatggt ttgcaggtaa
     3181 catgcagtca actggcctgt gggcaccggc tcagaaagag gtcaccatta agtccaatgc
     3241 gaacgttcct gtgaccgtca ccgtggcgct ggctgacgac ctgaccggac gtgagaagca
     3301 tgaagttgcg ctgaaccgtc cgccaagagt gactaaaacg tactctctgg acgctagcgg
     3361 tacggtgaag ttcaaggtgc cttacggtgg cctgatttat atcaagggca atagctctac
     3421 caatgaatct gccagcttca cctttactgg cgtggtaaaa gcaccgttct ataaagacgg
     3481 cgcatggaaa aacgatctga actcaccggc tccgctgggt gagctggaat cagacgcttt
     3541 cgtctatacc acaccgaaga agaacctgaa tgccagcaat tacactggcg gactgaagca
     3601 attcgctaac gatctggaca cctttgccag ctcgatgaat gacttctacg gtcgtgatga
     3661 aacgagcggt aagcaccgga tgtttaccta taaagcattg acgggacata aacatcgttt
     3721 cacaaacgat gtgcagatct ccatcggtga tgcgcactcg ggttatccgg taatgaacag
     3781 cagcttctcg acgaacagca ccacgctgcc gacgacgccg ctgaacgact ggttgatctg
     3841 gcacgaagta gggcacaacg ccgcagaaac gccgctgact gtaccgggtg caactgaagt
     3901 cgctaacaac gtgctggcgc tgtacatgca ggatcgttat ctcggcaaga tgaaccgtgt
     3961 cgctgacgat attaccgttg cgccggaata tctggaggag agcaacggtc aggcatgggc
     4021 acgcggcggt gcgggtgacc gtctgctgat gtacgcgcag ctgaaggaat gggcagagaa
     4081 aaactttgat atcaagaaat ggtatccaga tggcactcct ctgccagagt tttacagcga
     4141 gcgtgaaggg atgaaaggct ggaacctgtt ccagttgatg caccgtaaag cgcgcggcga
     4201 tgatgttggc aacaagacct ttggcggcaa gaattactgt gctgaatcca acggtaacgc
     4261 tgcggacacg ctgatgctgt gtgcctcctg ggtcgcccag acggatcttt ccgcattctt
     4321 taagaaatgg aatccgggcg cgaatgctta ccagttgccg ggagcgacgg agatgagctt
     4381 cgagggcggt gtgagccagt cggcgtacag cacgctggcg tcactcaagc tgccgaaacc
     4441 ggaacaggga ccggaaacca ttaacaaggt taccgagcat aagatgtctc tcgagtaatc
     4501 ctgtatgccg gataaggcgt tcacgccgca tccggtatga aataaggcgc atcgggcctg
     4561 gaggaaagcc gtatggcata cacctgcagg cccgcatccg gcaagttaca acaaacaacc
     4621 tttaaccatg ctttttgatg tttttcagca ataccccgcg gcgatgcccg tcctggcaac
     4681 cgtcggagga ttgatcatcg gcagtttttt gaatgtggtg atttggcgtt accccatcat
     4741 gctgcgccaa caaatggcgg agtttcacgg tgaaatgtcg agtgcgcagt caaaaataag
     4801 ccttgcgctg ccgcgttcgc actgtccgca ttgccagcag actatccgcg ttcgtgacaa
     4861 tattccgctg ctctcctggt tgatgctcaa agggcgctgc cgcgactgtc aggcgaaaat
     4921 cagcaagcgt tatccgctgg tggagttatt gacggcactc gcttttttgc tggcgagtct
     4981 ggtctggccg gaaagtggat gggcgctggc ggtgatgata ttatccgcct ggctgattgc
     5041 cgcgagcatc atcgacctcg atcaccaatg gctgcccgat gtttttactc agggcgtatt
     5101 gtggacagga ctgattgcgg catgggcgca acagagtccg ttaacgctac aagacgcagt
     5161 taccggcgtc ctggtggggt ttatcacttt ttactccctg cgctggatag ccggaatagt
     5221 tctgcgtaaa gaagcattag gcatgggcga tgtattattg ttcgctgcgt taggtggctg
     5281 ggtggggccg ttgtcgctac ccaatgttgc tttaatcgcc tcatgctgcg gcctgatata
     5341 tgccgttatt acaaaaagag gatcaaccac actgcctttt ggaccgtgtt taagtctggg
     5401 cggtatagca acaatttatc tacaggcatt gttttaatga taacaacgtc attatcaaag
     5461 tgacatttta actcttatta ataaccttag agattattta ccatgtcgat aaaacaaatg
     5521 ccagggaggg tattaatatc gctattgttg agcgttacag gattattaag tggctgtgcc
     5581 agccataatg aaaatgccag tttactggcg aaaaaacagg cgcaaaatat cagccaaaac
     5641 ctgccgatta aatctgcggg atatacctta gtgctggcac aaagtagcgg cacgacggta
     5701 aaaatgacca ttatcagcga agcggggact cagaccacgc agacgcctga cgccttttta
     5761 accagctatc aacgacaaat gtgcgctgac ccaacggtga aattaatgat taccgaggga
     5821 attaattaca gcataacgat taatgataca cgtacaggta accagtatca gcggaaactg
     5881 gatcgtacca cctgtggaat agtcaaagca taacgtcggg tagatataaa ttggcgcggg
     5941 ttgtttttcg tgacgcacga atttatctca ttcaatggct gacaaaaatt cgtcacactc
     6001 ttaaccagag acaatctttt aatacagaca aagagcatct gcgaaaaatt gtacgcggga
     6061 tgttctggct gatgctgctt attatttctg caaaagtggc gcattcactc tggcgctatt
     6121 tctccttttc tgcggaatat acggctgttt ccccatcggc gaataaaccg ccccgtgcgg
     6181 atgcaaaaac gttcgataaa aatgacgtgc aattaatcag ccagcaaaac tggtttggca
     6241 aatatcagcc cgtcgccacg ccggtaaaac aacccgaacc tgtgcctgtg gcagaaacgc
     6301 gtcttaatgt ggtgctgcgc gggattgcct ttggtgccag acccggcgcg gttattgaag
     6361 aaggcggtaa acagcaggtc tatttgcagg gtgaaacgct tggctcgcac aatgcggtga
     6421 ttgaggaaat caatcgcgac catgtgatgc tgcgctatca gggaaaaata gagcgcctga
     6481 gcctggctga agaggagcgt tccactgttg ccgtgaccaa caaaaaagct gtcagtgacg
     6541 aagcaaagca agctgttgct gagcctgctg ccagtgcgcc agttgagatc ccgactgccg
     6601 tgcgtcaggc actggcgaaa gatccgcaga aaatttttaa ctatatccag cttacgcctg
     6661 tgcgtaagga ggggattgtc ggttatgcag tgaagccggg ggcagatcgt tctctgttcg
     6721 atgccagcgg tttcaaggaa ggcgatatcg ccattgcgct aaatcagcag gatttcactg
     6781 atccacgagc aatgattgct ctgatgcggc agttaccttc aatggattcc attcaactta
     6841 cggttttacg caagggtgcg cgccacgaca tttccatcgc gctgcgctaa ccgcatttaa
     6901 tccaggagaa tcattcatcg tgttttggcg tgatattacg ttgtcggtct ggcgtaagaa
     6961 gacaactggc ctcaaaacaa aaaagcgttt actgccgctg gtgctggcag cggcattatg
     7021 tagttcaccg gtctgggcgg aagaagccac tttcaccgct aattttaaag ataccgacct
     7081 gaaatcgttc atcgaaaccg tcggcgctaa ccttaataaa accatcatta tggggccggg
     7141 cgtacagggg aaagtgagta ttcgcaccat gaccccgctc aatgaacgcc agtattacca
     7201 gctattcctt aacctgctgg aagcacaggg gtatgccgtc gtaccaatgg aaaacgacgt
     7261 gctgaaggtg gtgaaatcca gcgctgcgaa agtcgagccg ctgccgctgg tcggtgaagg
     7321 cagcgacaac tacgcgggcg atgaaatggt caccaaagta gtgccggtac gtaatgtgtc
     7381 ggtacgcgag ctggcaccga ttctgcgcca gatgatcgac agcgcaggct caggcaacgt
     7441 tgttaattac gatccctcca acgtgattat gctcaccgga cgcgcctccg ttgtggagcg
     7501 gctgacggaa gtgatccagc gtgtggatca cgcgggtaat cgcactgaag aggtgatccc
     7561 gctggataac gcctcggctt cggaaatcgc ccgcgtgctg gaaagcctga cgaaaaacag
     7621 cggcgagaac cagcctgcga cgctgaaatc tcaaattgtc gccgacgaac gcaccaacag
     7681 tgtgattgtc agtggtgacc cggccacgcg ggacaaaatg cgccgcctga tccgtcggct
     7741 ggactcagaa atggaacgca gcggcaacag ccaggttttc tatctcaaat acagcaaagc
     7801 cgaagatctg gtcgatgtgc tgaagcaggt cagcggcacg ctcacggcgg ctaaagaaga
     7861 ggcggaaggc acggtcggta gcgggcgtga gattgtatcc atcgccgcca gcaaacacag
     7921 taatgccctg attgtaaccg caccgcagga catcatgcag tcgctgcaaa gcgtgattga
     7981 acaactggat attcgccgtg ctcaggtgca cgtcgaggcg ttgatcgtgg aagttgccga
     8041 aggtagcaat atcaacttcg gcgtgcagtg ggcgtcgaaa gatgccggat taatgcagtt
     8101 tgctaatggt acgcagatcc ctatcggtac gctgggggca gccatttctc aggcgaaacc
     8161 gcagaaaggc tcgacggtga tcagtgaaaa cggcgctacc accattaatc cggataccaa
     8221 cggcgatctc tccacactcg cccagcttct ttctggattt agcggtacgg cggttggcgt
     8281 ggtgaaaggc gactggatgg cactggtgca ggcggttaaa aacgactcca gctcgaacgt
     8341 tctctccacg ccgagcatca ctacgctgga caaccaggaa gccttcttca tggtgggcca
     8401 ggacgttccg gtattaaccg gctcaaccgt tggctccaat aacagcaatc ctttcaatac
     8461 agtagaaagg aaaaaagtcg gcatcatgct gaaagtcacg ccgcagatta acgaaggtaa
     8521 cgcggtacag atggtgattg agcaggaagt ctcgaaggtg gaaggacaga ccagcctcga
     8581 cgtggtgttt ggtgagcgca aactgaaaac caccgtgctg gctaacgatg gtgagctgat
     8641 tgtgcttggt ggtctgatgg acgatcaggc gggagaaagc gtggcgaaag tgccgctgct
     8701 gggcgatatc ccattgattg gtaacctgtt taaatcgacg gcggataaaa aagaaaaacg
     8761 taacctgatg gtgtttatcc gcccgaccat tctgcgtgac ggtatggcgg cagacggcgt
     8821 gtcgcagcgc aaatataact acatgcgcgc cgagcagatc tatcgcgatg agcaaggttt
     8881 aagcctgatg ccgcacaccg cgcagccggt actgccagcg caaaatcagg ctctaccgcc
     8941 ggaagttcgt gcgttcctta atgccgggag aacgcgttaa tggtgcctgt agcacaggaa
     9001 accaccgcta acaccgtgcg tctgccctac agtttcagcc gtcggtttag cctggtggca
     9061 tggtgcgaag cgtcgctgga gatcctccat gttcatccgt tgtcgctctc tgttttgcag
     9121 gagctgcagc gggggctgaa cgcgcccttt acgctgcggc aaatcgacga agccgaattt
     9181 gaacagcggc tgaatgcggt ctggcagcgg gactcttccg aggctcgcca actgatggaa
     9241 gatctcggtt ctgccgagga cttttttacc ctcgcggaag aactgccgga aacggaagat
     9301 ctgctggaaa gcgacgacga tgcgccgatc atcaaactga tcaacgccat gctggcagag
     9361 gcaatcaaag aaggcgcttc ggatatccac attgagacgt ttgaaaagag tctggtgatc
     9421 cgttttcgtg ttgacggcac attacatgaa atgctgcgtc cggggcgcaa actggcctcg
     9481 ctgctggtgt cgcgtatcaa ggtgatggcg cggctggaca ttgccgaaaa gcgcgtgccg
     9541 caggatggac gtattgcgct gttgctgggc ggccgggcga ttgacgtgcg tgtctccacc
     9601 atgccttctg cctggggcga gcgcgtggtg ctgcgactgc tggacaaaaa ccaggcccgc
     9661 ctgacgctgg agcgtctggg gcttagccag caactgaccg cgcagttgcg ccagctgtta
     9721 cacaaaccgc acggcatctt tctggtgacg gggccgacgg gttccggcaa aagcaccacg
     9781 ctgtacgccg gattgcagga gctgaacaac cattcgcgca acattctcac ggttgaagat
     9841 cccatcgaat acatgattga agggatcggt cagacgcagg ttaacacccg cgtcggcatg
     9901 acctttgccc gtgggctgcg cgcgattttg cgtcaggacc cggatgtggt gatggtcggt
     9961 gaaatccgcg ataccgaaac cgcagaaatc gccgtccagg cttcacttac cggacacctg
    10021 gtcctttcca cgctgcatac caacacagcg gtgggggcga tcacacgttt gcaggatatg
    10081 ggcgtggagc ctttcctgct ctcttccagt ctgacgggcg tgatggcgca gcgactggtt
    10141 cgcacgctgt gtcccgactg ccgccagccc gcaccagcca ctgacgaaga aaaacgcctg
    10201 ctgggaatta ccgacgcccg taccgtcact ctgtaccatc cacagggctg tcccgcctgt
    10261 aatcacaaag gttttcgcgg acggactgcc atccatgagc tgatcgtggt ggatgccaca
    10321 ttgcgtgatt tgatccaccg tcaggccggg gagctggagc tggaacgtta tgtccgacaa
    10381 cactctgcgg gtatccgcag caacggcatt gagaaagtgc tcgccggaga aacctctctc
    10441 gatgaagttc tgcgggtaac catggaggcg taatggcact gttttactat caggcgctgg
    10501 agcgtaatgg tcgcaaaacc aaaggtatga ttgaggcgga ttccgcgcgt catgcccgcc
    10561 agttgttgcg cggtaaagag cttatccccg tgcatattga agcccggatg aatacttcgt
    10621 caggggggat gttgcagcgt cggcggcacg cacatcgtcg cgtggcggcg gcagatcttg
    10681 cgctgttcac gcgccaactg gcaacgctgg tacaggcagc aatgccgctg gaaacctgct
    10741 tacaggcggt cagtgagcaa agtgaaaaac tgcatgtaaa aagcctcgga atggcgctgc
    10801 gcagccggat tcaggaaggt tacaccctgt cggacagcct gcgcgaacat ccccgcgtct
    10861 ttgattctct gttttgttcg atggttgctg ccggagaaaa atccgggcat ctcgacgtgg
    10921 tgcttaatcg cctggcagat tacaccgaac agcgacagcg cctgaaatca cgtctattgc
    10981 aggccatgct ctatccgctg gttctgctgg tggtggcaac gggcgtagtc actattttgc
    11041 tgacggcagt ggtgccgaaa atcatcgaac agtttgatca tctcggacac gcgctacccg
    11101 cctcaacccg cacgcttatt gccatgagcg acgcgttaca ggccagcggc gtttactggc
    11161 tggcgggttt gctggggctt ctggtgctgg ggcaacggct acttaaaaat cctgctatgc
    11221 gcctgcgctg ggatcaaacc gtgctgcgcc tgcctgtgac ggggcgtgtt gcgcgcgggc
    11281 tgaatacggc gcgtttttcc cgcaccttaa gcatcctcac cgccagcagt gttccgctac
    11341 tggaaggtat tcagactgct gccgccgtgt cggcaaatcg ctatgtcgaa caacaactac
    11401 tgctggcggc agatcgcgtc cgcgaaggaa gcagtctgcg cgccgcgctg gcggagttgc
    11461 gcctgttccc gccgatgatg ctgtacatga tcgcctccgg cgaacagagc ggcgaactgg
    11521 aaaccatgct tgagcaggcc gcagtcaacc aggaacggga atttgatacc caggtggggc
    11581 tggcgttagg gctgtttgaa ccggcgctgg tggtgatgat ggcgggcgtg gtgctgttta
    11641 ttgtcatcgc catcctcgag ccgatgctgc aactgaataa tatggttgga atgtaattta
    11701 cggagttatc acatgaattc gttatcccgc acacaaaaac cacgggcagg ttttaccctg
    11761 ctggaagtga tggtggtgat tgttattctt ggcgtcctgg caagtctggt ggtgcctaac
    11821 ctgttgggca acaaagagaa agccgatcgg caaaaagcca tcagcgatat cgtggcgctg
    11881 gaaaacgcac tggatatgta ccgactggat aacgggcgtt atccgaccac agaacagggg
    11941 cttgaggcgc tgatccagca gccagccaat atggcggact cccgcaacta ccgtactggt
    12001 ggatacatta aacgactgcc aaaggatccg tggggcaatg attatcagta tctcagtccg
    12061 ggtgaaaaag ggctgtttga tgtttatacc ctgggggcgg atggtcagga aaacggggaa
    12121 ggcgctggcg cagatatcgg taactggaat ttgcaggagt ttcagtaagc agtgcctgaa
    12181 cgcggattca cacttctgga aatcatgctg gtgatttttc ttatcggcct tgccagtgcg
    12241 ggcgtggtac agacttttgc gaccgattct gaatcgcccg cgaaaaaagc ggcgcaggat
    12301 tttctgactc gctttgcgca gtttaaggac agggcagtga tcgaagggaa aacactcggt
    12361 gtgctaatcg acccgcctgg ctaccagttt atgcagcgtc gtcaggggca gtggtcgccc
    12421 gtttcctcga cccggttatc ggcacaggtt acggtgccga aacaggtgca gatgctgtta
    12481 caacccggaa gtgatatctg gcagaaggaa tatgcgctgg agctgcaacg tcgtcgactg
    12541 acgctgcacg atattgaact tgagctgcaa aaagaggcga aaaagaagac gccgcagatc
    12601 cgtttttcgc cttttgaacc cgccacgccg tttacgctgc gcttctactc ggcggcgcaa
    12661 aacgcatgtt gggcggtaaa gctggcacac gatggcgcgt tatccctcaa tcaatgtgat
    12721 gagaggatgc catgaagcgc ggatttacct tgctggaagt gatgctcgcg ctggcgattt
    12781 ttgcgctggc tgccacggcg gtgttacaga ttgccagcgg tgcgctgagt aatcagcaaa
    12841 ttcttgagga aaaaacggta gcgggctggg tggctgaaaa ccagaccgcg ctgctctacc
    12901 tgatgacccg cgagcagcgg gcggtcaggc agcagggcga gagcgatatg gcaggaagcc
    12961 gctggtactg gcgaaccaca ccgctgagta caggcaacgc gctgctccag gcggtggata
    13021 ttgaagtcag ccttcacgaa gacttttcgt cggtgattca gtcacgacgc gcctggttta
    13081 gcgccgtggg aggccaacag tgagaaggac tcgcgctggt ttcacgttac tggaaatgtt
    13141 ggtggcaatt gccatttttg cctcgctggc actgatggcg cagcaggtga ctaacggcgt
    13201 cacgcgcgtg aatagcgccg tcgccggaca cgatcaaaaa ctaaacctca tgcagcaaac
    13261 gatgagtttt ctgacccacg atctgaccca aatgatgccg cgtccggtaa gaggcgatca
    13321 gggccagcgt gaaccagcgt tactggctgg cgctggcgtg ctggcgtctg agagtgaagg
    13381 aatgcgtttt gtgcgcggcg gtgtggttaa cccgttgatg cgtctgccgc gcagtaatct
    13441 gctcaccgtc ggttaccgca ttcatgacgg ttatctcgaa cggttagcct ggccgctgac
    13501 cgatgccgca ggcagcgtga agccgacaat gcaaaagttg attccggccg attcgctccg
    13561 tttgcagttc tacgacggca cgcgctggca ggagagctgg tcgtcagtac aggcgatccc
    13621 tgtggcggta cgcatgaccc tgcattcgcc gcaatggggc gagattgaac gcatctggtt
    13681 gttacgcggg ccgcaataat catgatcacc tcaccaccaa aacgcggaat ggcactggtc
    13741 gtggtgctgg tattgctggc agttatgatg ctggtaacca tcacgctttc cgggcggatg
    13801 cagcagcaac tgggaagaac gcgcagccag caggagtacc agcaggcgct gtggtacagc
    13861 gccagtgcag aaagcctggc gctgagcgcg ctcagtctga gcctgaaaaa tgaaaagcgc
    13921 gtgcatctgg aacagccgtg ggcttccggc cctcgttttt tcccactgcc gcaggggcaa
    13981 atcgccgtca ctctgcgtga cgcacaggcc tgctttaacc tgaatgccct cgctcagccc
    14041 acaacggcgt cgcgtccgct cgcggtacaa caactgattg ccctgatcac gcgcctggat
    14101 gtgcctgctt atcgggccga actgatagcc gaaagcctgt gggagtttat tgatgaagac
    14161 cgcagcgtgc agacgcgtct gggccgtgaa gacagcgaat atctcgcccg ctcggtgcct
    14221 ttctacgccg ccaatcaacc gctggccgat atcagcgaga tgcgcgtggt gcagggaatg
    14281 gacgccgggc tttatcaaaa actgaaaccg ctggtctgtg cgctgccgat gacccgccag
    14341 caaatcaaca tcaatacttt agacgtcacg caaagtgtga ttcttgaggc gctgtttgac
    14401 ccgtggttaa gccctgttca ggcgcgggcg ttattacaac aacgtccggc gaagggctgg
    14461 gaagatgtcg atcagtttct tgcacagccg ctacttgctg acgtcgatga gcgtactaaa
    14521 aaacagctaa aaaccgtcct gagcgtggac agcaattact tctggctgcg ttcagatatc
    14581 accgtgaatg agattgaact gacgatgaac tcgttaattg tccgcatggg cccacaacac
    14641 ttttcggttc tctggcatca gacaggagaa agtgagtgag ttccatcctt gagatttttt
    14701 tcccgctttg cgccgctgat cccatccatt ggcagcgccg tacacccgat gtggagcacg
    14761 gtatctggtc tgacgttgct aacgaacagc tccagcaatg gctgcaaact gatgcgattc
    14821 gactctacat tcccggcgaa tggatcagcg tctggcaggt tgaactgcct gatgtcgccc
    14881 gtaagcagat accgaccatt ctgcccgcct tactggaaga agagctgaac caggatatcg
    14941 atgaactgca tttcgccccg ttgaatatcg accagcaact ggcaaccgta gcagtgattc
    15001 accaacagca tatgcgcaac attgcgcagt ggttgcagga aaacggcatc acccgcgcta
    15061 ccgtcgcgcc ggactggatg tccattcctt gtgggtttat ggctggcgat gcgcaacggg
    15121 ttatctgccg catcgatgaa tgccggggat ggagcgccgg gctggcgctg gctccggtca
    15181 tgttccgcgc ccagctcaat gagcaggatt taccgctttc gctaaccgta gtcggcattg
    15241 caccggaaaa gctatcagca tgggctggcg cagacgctga acgcctgacc gttacagctc
    15301 tgcccgccat taccacttat ggcgaaccgg aagggaacct gctgacaggg ccgtggcagc
    15361 ctcgcgtcag ctaccgaaaa cagtgggcgc gctggcgggt gatgattctg ccgatattgc
    15421 tgattctggt tgcgctggca gtggagcggg gcgtgacgtt atggagcgtc agcgaacagg
    15481 tggcgcaaag ccgcacccag gcggaggaac agttcttaac gttgttcccg gagcagaagc
    15541 ggattgtgaa tttacgctct caggtgacga tggcgctgaa aaaatatcgc ccacaggccg
    15601 acgatacccg gctgctcgcc gagttgtcag cgatagccag caccctgaaa tcagcgtcac
    15661 tttccgacat cgaaatgcgt ggttttacct ttgatcaaaa acgccagata cttcacctcc
    15721 agctacgggc cgcgaacttt gccagtttcg acaaactgcg tagtgcactg gcaaccgatt
    15781 atgttgtgca acaggacgcg ttacagaaag agggtgatgc ggtttccggc ggcgtaacgt
    15841 tgcggaggaa ataacatgtt acgcgataaa tttattcact attttcagca atggcgtgaa
    15901 cgccagttaa gccgtggcga acactggctg gcacaacacc tggcggggcg ttctccgcgt
    15961 gaaaagggca tgttactggc agcggtggtg ttcctgttta gcgtcggata ttacgtcctc
    16021 atctggcagc cgttgagcga acggatagaa caacaggaga caatgttgca gcaactggtg
    16081 gcgatgaaca ccagactgag gagcgccgcg ccggatatta tggcggcgcg aaaatccgcc
    16141 acaacaacgc ctgcgcaggt atcgcgggtt atcagtgaca gcgcttccgc gcactcggta
    16201 gtcatcaagc ggatagccga gcgtggggag aatattcagg tctggataga tccggtggtg
    16261 tttaatgacc tgctgaactg gttaaacgcg ctggatgaaa agtatgcgct gcgggtgaca
    16321 caaattgatg ccagtgctgc tgagaagact gggatggtga atgtgcagcg gctggagttt
    16381 ggacgggggt aaagtatcag gagattagta tcaataaaat tactctcgtt aacgtggcga
    16441 gagtaattcc cccagacttt ataagcaaac ttttttcgtg aaggttattg tcagatccaa
    16501 aagcaaaaac ccgccttgtg ggcgggttct taagaattag tggtgcccgg actcggaatc
    16561 gaaccaagga cacggggatt ttcaatcccc tgctctaccg actgagctat ccgggcaacg
    16621 gggcgcatta aacctgattc gcctcgtctc gtcaatcaaa tttcttcaat ttactgcaga
    16681 ctgcacaatc tatcatcact ttgccgctat tgcacgcatt gtcaggcaaa ccaggcggtc
    16741 caggcggcgg aaagcggcat tgccagaagg agtgctggaa gcatgttgac caccgggaac
    16801 attttaatgc cacagatgcg taatccggtt gccagcagca ataaaccgcc tacggcgctg
    16861 aagtctgcca tcatcgacgg tgtggtcagc ggtaatatca gcgcggcagc ccacgccagc
    16921 gttaactgga tgatcagtaa tgggatacta atcaccgata ccgcaatacc cagcgagcag
    16981 gcgaagatca tcgccgtaaa gaaatcaaga aatgacttgg cgattaaaat actcggatcg
    17041 ccggtcatcc cttcgttcat cgccccgaag atcccggtgc cgctggcgca aaacaggaca
    17101 ataatcgcga cataattctg aataaaagat tcatgcgctg gcttcttacg tgagtgacga
    17161 aacagatttt gtgctttggt gactgctgta ttgacacctt tctccagcaa acaaatttcg
    17221 ccaattagag cgccaagtag cgtagctaaa accatcgctg gaaggttggc acatttcacc
    17281 accagtaaaa taccaatccc cagcgatgcc agaccaaaaa tagatgtcat ggagacgcgg
    17341 atacgttccg gtaagcgttg gctgagcagt gcgccgagaa cgccacccag taatactgca
    17401 ctagcgttga taaaagggcc gatgaccaca agagttcctg ttaactgtat tgtttgattt
    17461 tcttattatg accgtttggt gccgtcgatg gcttgtttgt tcgcaaagtc ctggcttgca
    17521 cgctttagcg aaaggtgcca tgattgcgcg aattttctcc tctctgtacg gagtttgccc
    17581 gatgcacgcc atctccttac attctctcgc ttatcgccgt ttcgcgcgaa acgtttccct
    17641 ttctatgtta ctgctcatgc ggtgaagtta acgcacgctc actgcaggac aacagtaaaa
    17701 tcagagcgtt tctgctttta ctgatgtctg gcggtcggag ctggtgacca gtttgaccca
    17761 tatctcatgg ggcagggttt tccaccttgt ccggtattct tacttccccg aaacgggttt
    17821 gcgcttatga aatcaatgaa tattgccgcc agtagtgatc tggtatcccg actttcttct
    17881 catcgtcgcg tggtggcgtt gggagatact gattttacgg acgtcgcggc agtcgtcatt
    17941 accgctgcgg atagtcgcag tggcattctt gcgttgctta agcgcaccgg ttttcatcta
    18001 ccggtgtttt tgtattccga acatgctgtt gaattacctg cgggcgttac ggcggtaatc
    18061 aacggcaacg agcagcagtg gctggagctg gaatccgcag cctgtcagta tgaagagaat
    18121 ttgctgccac cgttttatga cacgctgacg cagtacgttg agatgggcaa cagcaccttt
    18181 gcttgccctg gacatcaaca tggtgcgttt tttaaaaagc atcctgccgg acgccatttt
    18241 tacgatttct ttggtgagaa cgtctttcgc gccgatatgt gtaacgctga cgtaaaattg
    18301 ggcgatctgc ttattcatga aggatcggcg aaagatgcgc agaaattcgc agccaaagtc
    18361 tttcatgccg ataaaaccta ttttgtgctg aacggcacat cggcagcgaa taaagtggtg
    18421 acgaatgcgc tgttaacgcg tggcgatctg gtgctcttcg accgtaacaa ccataagtcg
    18481 aatcatcacg gcgcgctgat tcaggcgggg gcgacgccgg tctatctgga agcttcacgt
    18541 aacccgtttg gtttcattgg cggtattgat gcgcactgtt ttaatgaaga gtatctgcgc
    18601 cagcaaattc gcgacgttgc gccagaaaaa gccgacctgc cgcgcccgtt tcgcctggcg
    18661 attatccagc tgggaaccta tgacggcact gtctacaacg cccgtcaggt gatcgatacc
    18721 gttgggcatc tgtgtgatta cattctgttt gattccgcgt gggtcggtta tgaacaattt
    18781 atcccgatga tggcggatag ctcgccgctg ctattagaac ttaacgaaaa cgatccgggg
    18841 atctttgtga ctcagtcggt gcacaaacag caggcgggat tctcacagac gtcgcagatc
    18901 cataaaaaag ataaccatat ccgcggacag gcgcgttttt gcccgcataa gcggttgaat
    18961 aacgccttta tgctccatgc ttctaccagc cctttctatc cgctgtttgc tgcactggat
    19021 gttaacgcca aaattcatga aggggagagt gggcgtcggc tgtgggctga gtgcgttgag
    19081 ttgggtattg agtcgcgcaa ggctattctt gcgcgctgta agctgttccg cccgtttatc
    19141 ccgcccgttg ttgatggcaa attgtggcag gattatccga catctgtgtt agccagcgac
    19201 cgccgttttt tcagttttga gccgggggcg aagtggcacg gctttgaagg atatgccgcg
    19261 gatcagtatt ttgttgatcc gtgcaagctg ttactcacta caccaggtat caatgccgaa
    19321 accggcgaat atagcgactt tggcgttccg gcgacgattc tggcgcacta tctgcgtgag
    19381 aacggcattg tgccggagaa gtgcgatctc aactccattc tgttcttatt aactccggcg
    19441 gaaagccacg agaagctggc acaactggta gagatgttgg cgcagtttga acagcatatt
    19501 gaggatgact cgccgctggc tgaggtgttg ccgagcgttt ataacaaata tccggtgcgc
    19561 tatcgcgact acaccctgcg ccagttgtgt caggagatgc acgatctgta tgtcagtttc
    19621 gacgtcaaag acctacaaaa agcgatgttc cgccagcaga gtttcccgtc agtggtgatg
    19681 aacccccagg atgcgcatag cgcttatatt cgcggtgaag tggagttggt gcggattcgt
    19741 gatgccgaag ggcgaattgc ggcagaaggg gcgttgcctt atccacctgg cgtgctttgc
    19801 gtggtacccg gggaagtctg gggtggggcg gttcaacgtt atttccttgc actggaagaa
    19861 ggggtgaatt tgttgccggg attttcgccg gagctgcaag gtgtttatag cgaaaccgat
    19921 gcggatggca tgaaacggtt gtacggttat gtgttgaagt aagaataaaa aaaacgggtc
    19981 accttctggc gacccgtttt tctttgcgta attagtggct aaccgtctgt gtgcctgtcg
    20041 ggacacgaac gtgtttatat ttgaacatcg ccatgaacgc gaaggccaga accacggagt
    20101 aaccagcgaa aatcaaccat acggtctgcc agtcggtaat gccgttttgg gtgtacatct
    20161 caacaacttt accgctcacg atgccgccga ggatacagcc gaagccgtta gtcatcatca
    20221 ggaacatccc ttgtgcactg gcgcgaattg ccgggctaac ttctttttcg acaaacaccg
    20281 aaccagagat gttgaagaag tcgaatgcgc aaccgtaaac gatcatcgac agtaccagca
    20341 gtacagtacc gaacggagtc gggtcgccgt aagcaaacag cgcaaaacgc aggatccacg
    20401 ccacaatact gatcatcatt acgttcttaa taccgtagcg gcttaagaag aacgggatgg
    20461 tcagaatgaa cagggtttca gagatctgcg aaatcgacat gatgattgac gcatgctgca
    20521 caataaagct gctggcaaac atcggatctt tgtcgaagct gtgcaggaag gtattaccga
    20581 acatgttggt aatctgcagt tccgcgccca gcagcattga gaagataaag aagattgcca
    20641 tacgcttgtt tttaaacagc gcgaatgcat cgaggcccag cagggttgtc cagctctgat
    20701 tcgcttgctg tttagcaacc ggaatatgcg gcagagtcag ggtaaacaga accagaatgg
    20761 cggaaagtgc tgcgccaata tacagctgca tgtggcttaa ttcgaagcca gacaggctca
    20821 ccacccacat tgccatgata aagccgatgg tgccccagat acggattggc gggaagtcag
    20881 taacgatatc catcccggca ttttgcaggc gatagtaaga gatggtgttg attaacccaa
    20941 gcgttggcat ataagcaaac gagttaatca atatcacaag gaacatcgcc tccggtgtcg
    21001 tgacctgtgc cgccatgaac agcgtgatag cgccaatggt gtggcaaatg gcatataccc
    21061 atttcgcact taaccatttg tcggccacaa tccccagcag cgcaggcata aagaccgcgg
    21121 cgatacccag tgagctataa actgcgccaa tagaagcacc gtcaaacttc agggtaacaa
    21181 acatatagga gccgagggtc gtcagccaac ttccccacag acagaactgc agaaaagaga
    21241 ggattttcag ctgcagctta agattcatgt taatttcctc acatcgtgat gcggatgagt
    21301 gtttgaaaaa gcacatttga ccgtgcgaga tgatgggatt ctatcaactt cgcggactgg
    21361 ttttttgtta cctgtggcaa ataattgcaa acattcccaa tttgcaaaac gaaattgtga
    21421 tgtggataac atttttgccc ctgagcatcg tcaggggcgg ttaatggaat tatcggcggc
    21481 ggtaggattt ttgcgcggta ttcactttat aaagataacg gcgagattcc gcagagggat
    21541 ggcgggtcgt cagcgtctga taaacatcgc ccggcgtcat ggtgttaata atattggcag
    21601 cctgaatctt atcattcgaa aagactcgca gcacgctgcc tgcgccgccg ttataggcgg
    21661 tgatgacggc ataacgccgc gatgttgggt tatcaattcc gccgagataa acattgttca
    21721 gcatcgccag atacgcggtg ccggtatcaa tattgctggc aggatcaaac aagaaactgc
    21781 ggctcggcgt gccggatttc ccctgcgagc ggaacacatc tttcccggca gtatgttgta
    21841 ccacctgcat taatcccagc gcatcggaac ggctgaccgc atacgggtta aaggaagatt
    21901 cggtctgcat aattgccaga atcagcgact catcaacgcc atatttccgt gacgcctggc
    21961 ggaccatgcc gagatatttg tgcgcacgtt tatcaaggtg gttcggtacc atgttaatgg
    22021 tgacgctgta gatgatacgc agcccgttac tgcggctttg cagacggttt ttcagcagat
    22081 aatccgcgaa gttgcttgcg cgaccttccc agcgaatcgg ctgcccggtg ttgtccacca
    22141 cctgaccgta aaggaaaggt tctttcgaaa tcgtaatatc atcaacgtcg gaatagagat
    22201 cgaccgaact cggatcgtca cccatcagca acgttttgat aattgcccgg cgcaaatgcg
    22261 cggcaggttc tgtcccggcg atagtttcga tagtaatcgt accgtcatcg aagttgatgt
    22321 ggctgcgggt ctgatattga tcggtgtatt tcacgtagtc cttaggaccg gcgatcacca
    22381 cctctttgaa tccccagatg ttctcaatat tgtgggcaaa ttgccccatc agaatatcaa
    22441 aaccgttggt atctttgacc caggcttcgt tataggtatc gccttttttg gtcgtcgaac
    22501 aggagatgag caacggcgca atcaaagcca gcgcgagata ttttttcatc attccgggtg
    22561 cgtgttgtgt ttgtttttat gcaagttgcc ggaggcgtgc tccggcactg tttttatttt
    22621 ttatcttccg gcgtatagcc ctcgatatgc acctctttac cctcgaacag gaagttgacc
    22681 atctcctgct ccagcagctt gcggtgctcg gcattcatca tgttgagttt cttttcatta
    22741 atcagcatgg tttgtttgtg ctgccactgc gcccaggctt ctttggagat ctcgttatag
    22801 atgcgttttc ccagctcgcc ggggtacagc tgaaaatcct gaccttctgc ttcacgttgc
    22861 aggaaagtac aaaaaatcgt tctgctcata aatcatcctc tttatcgact cacgcgctaa
    22921 accggcgcgc cagtgcgtaa ctgctgtaac aaacgctcca cgggagccgc caggccaact
    22981 gacggcggtt gcgctaagtt ataccagagc gcattgcctt catccatgca gccggtgaat
    23041 gacgacacgg gaagccacat aggcacaata tctaagtgga aatggctgaa ggtatgccga
    23101 aacgcggtca gctgcgtcag gttatcggca gcaatctgcc gttgcgccag ccactgccgc
    23161 aaactttctt cgtcggcaaa ctgcgggaaa cagtataaac cgccccacaa tccgctcggc
    23221 ggacgctgcg ccagcaatac ttcatcttcg tgctgtaaca ggagaaagta gccggtgcgc
    23281 tccggcagcg tctgtttcgg ttttttgccc ggataaagcg accagctatt gttggcggcg
    23341 gcaatacatc cgttttgtag cggacagagc gaacatttcg gtttcgagcg cgtacaaatc
    23401 atcgcaccca aatccatcat cgcctgatta aaccgttcca cgccaaccgc gggcgtcacc
    23461 tgctcgctca aactccataa tttattctcg acctcttttt tccctggcca gccgcttaca
    23521 gcatagcagc gcgccagcac ccgtttgacg ttaccgtcga gaatcggaaa gtgcttaccc
    23581 agagaaagcg agagaatcgc acctgcggtg gaacgcccga cgcccggtaa cgccgcgact
    23641 tcttcaaagg tttccgggaa tttaccgccg tgtaaggtcg ccacttgttg tgccgcttta
    23701 tgcaggttgc gcgcgcgggc gtaatagcca agcccggtcc acaagtggag aacttcgtcg
    23761 agcggcgcat tggcgagatc ggtcaccgtc gggaagcgcg ccataaagcg ttcaaaatag
    23821 gggataacgg tcgcaacctg agtttgttgc aacatcactt ctgagagcca tactttgtag
    23881 ggcgtcttgt caatttgcca gggcagcgtt tttcgcccgt atttatcgta ccagtccaga
    23941 acctgggctg aaaattgcga cgcttgcatg gtcaccgaat tcactgttgt tgggtgcaag
    24001 attgcagcac agcgacggca gggtgtaaac cggaactttc cgcagccacg gccttatcat
    24061 gcttgcatcc ggcaactaac tttggataat gcccgttttc agaacacttt cacaagcgac
    24121 taaatcttta tgaaaaacga cgtcatctca ccggaatttg atgaaaacgg ccgcccactg
    24181 cgccgtatcc gtagttttgt gcgccgccag gggcgactga ccaaaggcca ggaacatgcg
    24241 ctggaaaact actggccggt gatgggcgtt gagttcagcg aagatatgct ggatttcccc
    24301 gcgctttttg gccgtgaagc gccggtgacg cttgagattg gttttggcat gggggcgtcg
    24361 ctggtggcaa tggctaaaga tcgccccgag caggacttcc tcggcattga agtgcattca
    24421 ccgggcgttg gtgcgtgcct ggcttctgcg catgaagaag gtttaagcaa cctgcgcgtg
    24481 atgtgtcacg atgcggttga agtgctgcat aaaatgattc ctgacaattc attgcgcatg
    24541 gtgcagctct ttttccctga cccgtggcac aaagcgcgcc ataataaacg ccgtatcgtt
    24601 caggtgccgt ttgccgaact ggtaaaaagc aaactgcagc tggggggcgt attccatatg
    24661 gcgaccgact gggaacctta tgcggaacat atgcttgaag tgatgtcttc tattgacggt
    24721 tataaaaacc tgtcagagag caatgattac gtaccgcgtc cggcatcacg tccggtgacg
    24781 aaatttgaac aacgtggtca tcgtcttggt cacggagtat gggacttaat gttcgagagg
    24841 gtgaaataat ggcaaagaac cgtagccgtc gtctgcgtaa aaaaatgcac atcgacgaat
    24901 tccaggaatt aggattttcg gtggcatggc gattcccgga aggtacatcg gaagaacaga
    24961 ttgataaaac cgttgatgat tttattaacg aggtcatcga accgaacaaa ctggcctttg
    25021 acggcagcgg ttatctggcc tgggaaggtc tgatctgcat gcaggaaatc ggcaaatgca
    25081 ccgaagaaca tcaggcgatt gtgcgtaagt ggctggaaga gcgcaaactg gatgaggtac
    25141 gcaccagcga acttttcgac gtttggtggg actaagaaag catacgggcg atgacaaatg
    25201 caaaactgcc tgatgcgcta cgcttatcat gcctggaaag atgcacgatc gagtaggcgg
    25261 gataaggtgt ttacgccgca tccggcatgg aaaacgcgta ctttgttatc aatctggggc
    25321 cagcaaatgc tggcctgatt ttttcttgag ggaagactat gatgcgcaaa atgctgctgg
    25381 cggcagcact ttcagtgacg gcaatgaccg ctcacgccga ctaccagtgc agcgtcacgc
    25441 cgcgtgacga tgtgattgtc agcccgcaaa ccgtgcaggt gaagggcgaa aacggcaatc
    25501 tggtgatcac gccagacggc aacgtgatgt ataacggtaa gcaatattcc ctgaatgccg
    25561 cccagcgcga gcaggcgaag gattatcagg ctgaactacg cagcacgctg ccgtggattg
    25621 atgaaggcgc gaaaagccgc gtcgaaaaag cccgtattgc gctggataaa attatcgttc
    25681 aggagatggg cgaaagcagc aaaatgcgca gccgtctgac caaacttgat gcgcagctga
    25741 aagagcagat gaaccgcatt atcgaaacgc gcagcgatgg cctgacgttt cactataaag
    25801 ccattgatca ggttcgcgcc gaaggccagc aattagtgaa tcaggcaatg ggtggaattt
    25861 tacaggacag cattaatgaa atgggcgcga aagcggtgtt gaaaagcggc ggtaacccat
    25921 tacagaatgt gctgggaagc ctgggggggc tgcaatcctc aatccaaacc gagtggaaaa
    25981 agcaggaaaa atatttccag cagtttggca aagatgtttg tagccgcgtt gtgactctgg
    26041 aagatagccg caaagccctg gtcgggaatt taaaataatc ctctatttta agacggcata
    26101 atactttttt atgccgttta attcttcgtt ttgttacctg cctctaactt tgtagatctc
    26161 caaaatatat tcacgttgta aattgtttaa cgtcaaattt cccatacaga gctaagggat
    26221 aatgcgtagc gttcacgtaa ctggaggaat gaaatggagt ttttcaaaaa gacggcactt
    26281 gccgcactgg ttatgggttt tagtggtgca gcattggcat tacccaatat caccatttta
    26341 gcaaccggcg ggaccattgc cggtggtggt gactccgcaa ccaaatctaa ctacacagcg
    26401 ggtaaagttg gcgtagaaaa tctggttaat gcggtgccgc aactgaagga cattgcgaac
    26461 gttaaaggcg agcaggtagt gaatatcggc tcccaggaca tgaacgataa tgtctggctg
    26521 acactggcga aaaaaattaa caccgactgc gataagaccg acggcttcgt cattacccac
    26581 ggtaccgaca cgatggaaga aactgcttac ttcctcgacc tgacggtgaa atgcgacaaa
    26641 ccggtggtga tggtcggcgc aatgcgtccg tccacgtcta tgagcgcaga cggtccattc
    26701 aacctgtata acgcggtagt gaccgcagct gataaagcct ccgctaatcg tggcgtgctg
    26761 gtggtgatga acgacaccgt actggacggt cgcgatgtca ccaaaaccaa caccaccgac
    26821 gtagcgacct tcaagtctgt taactacggt cctctgggct acattcacaa cggtaagatt
    26881 gactaccaac gtaccccggc acgtaagcac accagcgata cgccattcga tgtctctaag
    26941 ctgaatgagc tgccgaaagt cggcatcgtt tataactacg ctaacgcatc cgatcttccg
    27001 gctaaagcac tggtagatgc gggctatgat ggcatcgtta gcgctggtgt gggtaatggt
    27061 aacctgtata aatccgtgtt cgacaccctg gcaaccgccg cgaaaaacgg cactgcagta
    27121 gtgcgttctt cccgcgtacc gacgggtgct accactcagg atgctgaagt ggatgatgcg
    27181 aaatacggct tcgtcgcctc tggcacgctg aacccgcaaa aagcgcgcgt cctgctgcag
    27241 ctggctctga cgcaaaccaa agatccgcag cagatccagc agatcttcaa tcagtactaa
    27301 tcacctcgcc ccggtatcgt gccggggctt tttcacttca gacttacgtc cattgccaat
    27361 tttcattacc ctaatgataa tcaccggaat aaattattcc gcgtgaggtt tttcgggtga
    27421 aaaagcaatg gattgttggc acggcactgc ttatgttgat gactggtaat gcttgggcag
    27481 atggcgaacc gccaactgaa aatatcttaa aagatcaatt caaaaagcag tatcacggca
    27541 ttctcaagct tgatgctatc accttaaaaa atcttgatgc taagggtaat caggccacct
    27601 ggtcagcgga aggcgatgtc tcttccagtg acgatctcta tacctgggtc ggtcagttgg
    27661 cagattacga actgctcgaa cagacctgga cgaaagataa accggtaaaa ttctcggcga
    27721 tgttaaccag taaaggaacg cccgcgtctg gctggtcggt gaacttttac tcttttcagg
    27781 cggcagccag cgatcgtggg cgggtggtcg acgatatcaa aacgaataat aaatatctga
    27841 tcgtgaatag cgaagatttc aattatcgct ttagtcagct tgaatctgcg ttgaataccc
    27901 agaagaactc gattcctgcg ctggaaaagg aagtgaaagc gcttgataag caaatggtgg
    27961 cggcgcagaa agcagcggat gcatattggg gaaaagatgc aaacggtaag caaatgaccc
    28021 gcgaagaagc ctttaaaaaa attcaccaac agcgtgatga gtttaataaa cagaacgata
    28081 gcgaggcgtt cgcggttaaa tatgacaaag aggtttatca accggcgatt gcggcatgtc
    28141 ataaacagag tgaagagtgt tatgaagtgc cgattcagca gaaacgagat ttcgatatca
    28201 acgaacaacg gcgacagact tttctgcaat cacaaaaact cagccgtaaa ttgcaggatg
    28261 actgggtaac gctcgaaaaa gggcaatatc cgttaacgat gaaagtttca ggaataaaca
    28321 gtaaaaaagt cgcgattttg atgaaaattg acgatatcaa ccaagctaat gaacgctgga
    28381 aaaaagatac cgaacaactg cggcgcaatg gggtgattaa gtaagtttga tatagtgcac
    28441 gatttgtagc aggatgcggc gttgaccccg catccggcaa tcaacgtctg atgcgacgct
    28501 tgcgcgtctt atcagaccta caaccgttgc cacatgtagg ctggataagg cgttcacgcc
    28561 gcatccggca atacaagttt actcagccag aaaaagctcc agcagcgaat ttaaaaacag
    28621 cttcccatgt tccgttatct gccagtaatc cgcacattcg gtgagataac cctgggcaat
    28681 cgcctcgtct aactgtgggc gaatcacatc ttcgcaaagc ccggtatacg caataaactc
    28741 cacgcgcggc gcggcctcca gcagacggaa gcgattcata aagaactcaa acggcttatc
    28801 tgcggcttcg acatcacgct ggctttccag ataccttccc tgcataaaac cacgcggatg
    28861 acgcgttttg gtggtacgca gaatgcgccc atccgggaag gtcactttgc cgtgcgcgcc
    28921 acagccaata ccgatgtagt cgccaaatcg ccagtagttg agattgtgct ggcactgata
    28981 accgggtttg gcgtaagcgg aagtttcata ttgctgataa cccgctgcgg ttaataactg
    29041 atgcccctgt tcgaaaatat cccacagcgc gtcatcgtcc ggcagcaccg gtggtcgcga
    29101 accaaacagc gtattgggtt cgatggtcag ttgataccag gaaagatgcg gcggattcag
    29161 ttcaatggcc tggcgtagat cgccaagcgc ctcttccagt gattgatccg gtagtccatg
    29221 catcaaatca aggttaaagc tacgtaaccc taaaccgctc gccagctttg ccgctcgttt
    29281 cgcttcttgc gggccatgaa tgcgcccgag tcgtttcagc ttttcttcgc taaaactctg
    29341 cacaccaata gagatgcggt tcacaccagc acgctgataa tcgacaaagc gatccgcttc
    29401 taccgtgcct gggttcgctt ccatagtaat ttctgcatcc gctgccagcg gcaaacgcgc
    29461 acgcacgccg tccagcagcg tttgcatcgc cgggccggaa agcaggctcg gcgtaccacc
    29521 gccaataaag attgtcttta cttcacggcc ctgagcgtaa gccacatcgt tgtccagatc
    29581 gttaagcaga tgctgaacat aatcgtcgtg cggcacttct cctttcaacg cgtgagagtt
    29641 gaaatcgcag tacgggcatt tctgcacgca ccacgggatg tgaatgtaga gactcagcgg
    29701 cggtaattta accattacgt aaagcgtcca gcagcagttt caatgcctga ccacggtggg
    29761 aaatggcgct cttttcttcg cgggtcagtt cggcggcggt tttcccttcg gaaggtacga
    29821 agaagattgg atcataacca aagccaccag tgcccgctgg ttcacgagta atcacgcccg
    29881 gccagctacc gtggcatacc agcggagtcg gatcttccgc gtgacgcaga tataccagca
    29941 cgcagtggaa acgcgcctga cgttggtcat ccggtacgtc tttcagtgtt tccagcagtt
    30001 tttgcagatt ctttagatcg gtcgcgtctt caccggaata acgcgcggag taaatgcccg
    30061 gcgcgccgcc aagcacatct accgccagac cagagtcgtc ggcaattgcc ggtaaaccgg
    30121 tcacttttgc cgcatggcgc gctttcagaa tcgcgttttc gataaaggtc aggccggttt
    30181 cttcagcgga atctacgccg aggtctgttt gggccacgat atcaagaccg aagtcgctaa
    30241 gcagcgacgc cagctcacgc actttaccgg cattgccggt tgcgaggaca actttttgca
    30301 taggatacct aattaattaa cgccgcgatt tctggcggga tttgttgcgg attaatgatt
    30361 ttaatttgtt tgtggcggcc aagttcgcct ttttcaatca ccacctggct tttggcaacc
    30421 cggaattgct taccgagaaa cttcaccaga tgactgttgg cctgaccgtc aaccggcggc
    30481 gcggtaatgg cgactttaac ttcgtcgcca tgtaaaccga caatagaatc acggctggct
    30541 ttcggctgaa tatagagccg taaaaccaga ccgtcatcat taactgttac ggcactcata
    30601 acgccatcca cagccccggc agcagcatgt ttccggttgc ctgtaatact tctgcgacac
    30661 ccatattgat gacatacagc agcagaacga ggatcatcgg cgagaaatca atcccaccca
    30721 ttgccggtag caggcggcga atcgggcgca gcagcggatc ggccagctga atcagcacgt
    30781 attcaatcgg gctacgaccc tggcttaccc agctcataat cgccatcacc agcaggaccc
    30841 agaaaatcag caggccgatg gttttcagca gaatcagtaa accggcaatc cagatgattg
    30901 gcaggaaggt caccacttta aacagcacga tggctttgat aaaactgaga atataggcaa
    30961 ccagcagcga ggcgctgtca atggggccca ttgccggaat aacgcggcgc agtggcccga
    31021 taattggctg cgttaccttc actacaaact gtgagaaggg gttgtaaaaa tcacaatgag
    31081 cccactgcat ccagatgcgt aataacagca ccatggtata cagctcaatg accgttgaaa
    31141 gcaggaaagt caacgtattc atggcgttcc tttaattcct tattttttag agtaatcacg
    31201 cgcaccaaaa attgcagtgc cgatacgaac catcgtgcta cctgccgcaa tggcggcttc
    31261 catatcgtcc gacattccca gagagagcgt gtcgatatgc gggtagcgcg ttttcagtcc
    31321 ggcaaatgct acagccattt ggcgtgcaac ttcaaactgc cttacatatt ctgactcagg
    31381 tgcagggatt gccatcagcc cgcgcagacg taaacgcggt agttcagcga ccgcagctgc
    31441 cagctcgtcc agttctgcca gttgaatccc ggacttactg ttttcatcac taatattaat
    31501 ttgaatcagt acgttaagag ggggaagttc tgccgggcgc tgatcgttga ggcgggtagc
    31561 gatgcgcaaa cggtcgatgg tatgacacca gtcgaaatgc tctgccacca ggcggctttt
    31621 attagactgc aacgggccaa taaaatgcca ttctaatcct gttacgccca gttcctgaaa
    31681 gtggcgaatt ttatccaccc cttcctgaac gtagttttca ccaaattgac gctgcccggc
    31741 atcaatggct tctgcgatgg cgctcgcagg ttttgtttta ctgactgcaa gcagcgtaat
    31801 ttcttctgga gaacggccgc aacgcgttgc agccgctgag attttgtccc ggacctgtgc
    31861 caggttatgc gcaatatcgt tcattttccg aggatcttag tatgaatatg gaagaaattg
    31921 tggcccttag tgtaaagcat aacgtctcgg atctacacct gtgcagcgcc tggcccgcac
    31981 gatggcgcat tcgcggcaga atggaagctg cgccgtttga tgcgccggac gtcgaagagc
    32041 tactgcggga gtggctggat gacgatcagc gggcaatatt gctggaaaat ggccagctgg
    32101 attttgccgt gtcgctggcg gaaaaccagc ggttgcgtgg cagtgcgttc gcgcaacggc
    32161 aaggtatttc tctggcatta cggttgttac cttcgcactg tccacagctc gaacagcttg
    32221 gtgcgccacc ggtattgccg gaattactca agagcgagaa tggcctgatt ctggtgacgg
    32281 gggcgacggg gagcggcaaa tctaccacgc tggcggcgat ggttggctat cttaatcaac
    32341 atgccgatgc gcatattctg acgctggaag atcctgtgga atatctctat gccagccagc
    32401 gatgtttgat ccagcagcgg gaaattggtt tgcactgtat gactttcgca tcggggttgc
    32461 gggctgcatt gcgggaagat cctgatgtga ttttgctcgg agagctgcgt gacagcgaga
    32521 caatccgtct ggcactgacg gcggcagaaa ccgggcattt ggtgctggca acattacata
    32581 cgcgtggtgc cgcgcaggca gttgagcgac tggtggattc atttccggcg caggaaaaag
    32641 atcccgtacg taatcaactg gcaggtagtt tacgggccgt gttgtcacaa aagctggaag
    32701 tggataaaca ggaaggacgc gtggcgctgt ttgaattatt gattaacact cccgcggtgg
    32761 ggaatttgat tcgcgaaggg aaaacccacc agttaccgca tgttattcaa accgggcagc
    32821 aggtggggat gataacgttt cagcagagtt atcagcagcg ggtgggggaa gggcgtttgt
    32881 gacgttgcca tgcccgatgc gcaagtttat agggcctata aaggcaataa atgtctgatg
    32941 cgacgctaac gcgtcttatc agacctacag ccgctgccag atgtgtgctg gataaggcgt
    33001 tcacgccgca tccggcaaga tggcggtaac gactatccgc caggctttta gatcgcctta
    33061 atatccctgc tcgaaatagc tttcgagaat aataaccgca gaggcagagt cgactttgcc
    33121 tttgttgagc gcccgatagc cgccctgttc aaacagaccg gaacgggctt ccacggtgct
    33181 aagacgttcg tcatggagct ttacttcaac accgaaacga ccatgaatac ggttggcaaa
    33241 tttacgcgct ctggcagtca atggttgctc ggtgccgtcc atattcagcg gcaaaccgac
    33301 gatgatttcg tccggctgcc actctttcag taaacgctcg ataatgttcc agtccggcgt
    33361 accgtcctgt gctttaattg caggcaaagg gcgagcggtg ccggtaatgc gttggccgac
    33421 cgctacgcca atgcttttgg tgccgaagtc gaaggcgagt aaggttccac tcatcaggcg
    33481 tgccctgcca caccaggcat ggtgagaata tccacaccaa tcagttttgc cgcctcgcgc
    33541 cagcggtcgg caatcggcgt tttgaacaga atattcagat ccgccggggc cgttagccac
    33601 gcgttatcga gaatttcttg ttccagttga cctttttccc aggaggcata acccagagcc
    33661 accaatacgt cagacggttg tttatcggtg ccgagcgttt ccagcacatc gcgggaagtg
    33721 gtcatcaccg tgttgtctga aatgcgaatg ctggaagcaa aattggaggg cggagtatgc
    33781 aaaataaacc cgcgatcttc agccagtggg ccgccgagca taaccggttt atccagacgg
    33841 attgattcat cacgcggctc cggcgtgatc ttcagctttt ccagaatccc ttcaattttg
    33901 agattttcca gcggcttgtt gacgatgatc cccattgcac cattggtatt atgttcgcaa
    33961 atgtagacca cggaacgacg gaaaatcgga tcctggagag caggcatggc aataagaaag
    34021 tgatgctgta aattcattgt cagaggttct gtttcctggt tcaaaaaagc aacagcgccc
    34081 agtatgcgtg gaaagaaggg cgctgtcact cagagtctca acgagatcct tctcgctaag
    34141 gtgggttact gctgctgtaa acgtgcttcg atggcatcca ttaacattcc ggtgatcgac
    34201 accggaaact ctgcttcaat ctcacgaata caggttgggc tggtgacgtt aatttcagtc
    34261 agacggtcgc cgatgatatc cagaccaaca aaaatcagcc ctttttcttt cagcgtcggc
    34321 ccgatctgac gggcgatttt ccagtcactt tccgtcagcg gacgaggttc accgcgacca
    34381 ccggcagcca gattgccacg ggtttcgccc ccctgcggaa tacgcgccag gcagtacggt
    34441 accggctcgc catccaccac cagcacgcgt ttgtcgccat ctttaatggc tggcaggtaa
    34501 ttttgcgcca tgcagtagcg agtgccatgc tcagtcaggg tttcggcaat cacgccgagg
    34561 tttggatcgc cttctttcac gcggaaaatc gacgcgccgc ccataccgtc cagcggctta
    34621 agaatgatgt cgctgtgttt ctcccagaac gcttttagct gcgctttatt gcgcgtaacc
    34681 agcgtttctg gcgttaagtc agagaaccag gcagtaaaca gtttctcgtt acagtcgcgc
    34741 aggctctgcg gcttgttaac gatcagcgtc cctttctctt cggcacgttc cagaatatag
    34801 gtcgcgtaga taaactcggt atcaaacggc gggtctttac gcatcaggat cacatcgaga
    34861 tcggccagcg gcagatcctg ttcaccgacg aatgaaaacc actcttcgta gttctgcttc
    34921 acgttcagcg tgcgggtatg ggcgcgggct tcgccattga tcagatacag atcgcccatc
    34981 tccatatagt gaagttcgta accacgacgc tgtgcttcca gcaacatagc gaaactggaa
    35041 tctttcttga tgttgatgtt tgcgatgggg tccatcacga tgccgagctt gatcattatt
    35101 cttctccgtt agcccaaatc gccaaatcgt acttgtagcg cggtaatggc ggtgagcgca
    35161 gttgtctctg tacgcaaaac gcgaggtccc aacaggatat cagtaaattg atagcgggca
    35221 gtcatggcaa tttcatctgc cgataaaccg ccttccgggc caatcagcag gcggacgcgt
    35281 tcaaccggta acggcaacgt attgatgctg ttactggcgc gcgggtgaag attcagtttc
    35341 agtccttcat cctgctctgc acaccaggct tccagatcca tcgctgggcg gatttccggc
    35401 acccggttac gaccacactg ctcacaggca gcaattgcaa tcttctgcca ctgctggagc
    35461 ttcttgttca gacgttcact atccagttta acgccgcagc gctcagaaaa aagtggcgta
    35521 atgaggctta caccgagttc gatcgatttc tggatcgtaa attccatttt ttcaccacgc
    35581 gacatcacct gaccgaggtg aatatgcagc ggagattcgc gatcgtcgat cttgccttcc
    35641 agcaccttca cttccacgct ttttttgctg gcgctggtaa tttcggcgtc aaagacctgg
    35701 ttgctaccgt caaacaattg caacgcctgc cccggcccca tgcgcagtac gcgcccgata
    35761 tggttggcgg catcttcgca aagcgcgatg tgagaatggc tggtcagtgg ttcaggatga
    35821 taaatgcggg ggatacgcat agttaaaaat ccgcgtcgtc tccccacgcg gttgtacgcg
    35881 tggggtaggg gttaacaaaa agaatcccgc tagtgtaggt tagctctttc gcgcctggca
    35941 agcgcgttgc acatacgggt tatgattgcc ttgcaccttc gcgatgcgtt catcgcgctc
    36001 gcactcccag tcggtaaccg gatacatctt gttccatgcg ttgaacagct gcgtttgctg
    36061 gcgagagagt gtcaggttgt attggtcgcg catatagaag taggtgcgtg caatggcacc
    36121 gcgtgcacgc gctggtggtt cggcagcttt ttctttgaaa tcgaccttca tggcgcattg
    36181 accgtactgg ccttcgccgc cattccactg gctgtacata aaattgccgc gatcgccatt
    36241 cacctcaccg actgacggct gcaggttatg catatcgctt tccatcttgc gatagaccgg
    36301 atctttagcg cagtttttac gtccaccgtc ctgccagcac tggcgctggt gaccgaactg
    36361 ccaggcggga acgacgtgtt cccactctac gcggctggct cggttttcat ttttgcgcac
    36421 ctgatagccg cacgattgca gatcaacaac gccttttttg ccctgccagt taattttaca
    36481 tccgcaataa aacgtaccgg gcgcgtcagc gtggactttt accgccgcgg ctttcgcctg
    36541 agaaaaacta ttgatacctg cggccaacgc cgggccggaa aatgctgcgc tcagtaccac
    36601 cgcagcaata gacaaataac ggtacatcac ttactccgtg ttaaaacgag ccagcaacgt
    36661 agcgaaagtt gttttggtat gcaatcagcc agatcaggaa agttcctgat agttcagatg
    36721 gttatttcgc aaccagctgt tcaccgcagt gaacacagcg atagacggcc tcgccacgaa
    36781 ctacgcgatt atggcggcgt acggtaagct gatgctcctg gcacttgcag cggtagggga
    36841 aggtgttgcg acgcacggat tgcagttcga actgatgcgt acgacgggcg ggaacgccca
    36901 gcacgctttc catcatccac ttccactctt tgccatgtgg cgctacgcgg ccgaaatgtt
    36961 tccataccag caaatgtgcc agttcgtgcg gtaccacttc ttcaataaaa gcttcactgt
    37021 tttccaacag caaaacggga ttgaggcgga tttcatagct ttccagccag gctgttccgg
    37081 cggaggttcc gcgctgggtg taagagagtt ttggctccgg gtagttacgc cctagcttca
    37141 ggttggcctg ggcgagtttt tcccgcaggc ggcgcataac ggcctgctgg atggcgatag
    37201 ggagacggga tgttttcata gcggcagagg atagagcgaa gaaaagatga ctgcaagagg
    37261 tggcttcctc cgcgatggga ggaagcctga ggagattaat cgtgagcgcc gatttcgcgc
    37321 agtttacgac ctttcatcag attacgttca atatgttcca gcgaaacgtg tttggtttcc
    37381 ggtaccagcc acaatgtcag caggataaac agtacgttca gagccgcata cacccagaag
    37441 gtgttggcgt tacccagcgt gttgagcatg gtcaggaacg ttgcgccaac gatcatgttg
    37501 gcaatccagt tggtggcggt ggagcaggtg atgccaaaat cgcggccttt cagcggctga
    37561 atttcggagc acagtaccca aatcagcgga ccggcactca tggcaaaacc gacaataaac
    37621 atcagcagca tggcgatggc gaaatactgc gctgacggag agtgaatacc gatatgcatc
    37681 attgtaccga gtacgcccat gccagcagcc atcaccagga agcccagcgt tagcgttggt
    37741 ttacgtcccc agcggtcaac aaggccgatt gcgataaagg tggcaagtac gttggtcagg
    37801 ccgacaatca cggtccccca catttgctcg gtagtgttgg tataacccgc cagttcgaag
    37861 attttcggcg cgtaatacat gatgacgttc atcccggtga attgctgcat tacctgcaac
    37921 agtacgccaa ggaacaccgc gcggcggaag ttgctgttct ctttaaacag cgcccagcca
    37981 ctctgtttaa cctgcaaact ttcacggatt tcatccagtt cgcgtttcgc ttccgcgctg
    38041 gtgtcacgca ggcgtagcag cacgcgttcg gcatcaacaa aacggcgttt ggcggcaaac
    38101 caacgtgggc tgtctggcag gaagaagaca ccaatcagca gcaaaattgc cgggatgata
    38161 atcacaccca gcatccagcg ccatgcaccg gtgtagctga aggcggtatc agaaagataa
    38221 gcaccgagga tcccgatagt gatcatcaac tgatacatcg agatcatact gccacgaatt
    38281 ttttccggcg caatttcaga gaggtacagc ggtgcggtat aagaggccac acccaccgcc
    38341 agccccagta gaacgcggga aagaatcagt acttcaacgt ttggcgcagc cgcagagaac
    38401 agcgaaccgg caacaaacaa aattgcgccg atcatcaggc tctttttgcg cccgagttta
    38461 aaggagagcc agccgctgcc caccgcaccg actgccgcac cgaacatcat ggagcttacg
    38521 acccattctt gcgtatgcga agtaatctgg aattcatctg caataaacgg cagtgcgcca
    38581 gcaattacac cgatatccag gccaaagagt aatcccgcca gagcggcaag gaagcagacg
    38641 aaaaacgtca ttgccttgtt tgaccgcccc tgttttttag cgtcaggcat gatgccctcc
    38701 aatatggtta ttttttattg tgaattaaga taggtgagta cgacgtaaaa agatgtgaag
    38761 caaatcacat cagtgtaatc gcttacacta acatttatct tagttatggt ttaagtgcat
    38821 gaaaataatt gagttatatc atcatagatt aaacgctgtt atctgcaatt aagactttac
    38881 tgaaaagaaa tgtaacaact gtgaaaactg aataataata tgaaaatatt acaaatattt
    38941 ggctggtgtt attggtgtaa tcggtttcag tagtgatgat aatttgcgat aaaacgtgtt
    39001 gaggaagaat gatgttattg tgcgggcagc tttttgccgg atgcggcgtg aatgcctatc
    39061 caccgcggtg aggtaaaaga aaaaggccag cgcgaggctg gcctttgaaa gcaggtgaag
    39121 aaagattact tcagaccggc agcatcgcgc agcagctgcg ctttgtcggt tttttcccac
    39181 gggaaatgtt cacgaccaaa gtgaccgtat gctgcggttt ctttgtagat cgggtgcagc
    39241 agatccagca tctgaatcag accgtatggg cgcaggtcga agaactcacg taccagcaga
    39301 gtcagttgtt cagaaggcac tttctcagta ccgaaagttt ctaccatgat ggaagtcggt
    39361 tcagccacgc cgattgcgta ggaaacctga atttcacaac gatcggccag gccagcagca
    39421 acgatgtttt tcgcgacata acgtgctgcg taggctgcgg aacggtccac ttttgatgga
    39481 tctttaccag agaatgcacc gccaccgtga cgcgccatgc cgccgtaggt atcaacgata
    39541 attttacgac cagtcagacc gcagtcaccc attgggccac cgataacgaa acgaccggtc
    39601 gggttgatga agaatttggt ggcagaagtc agccattcag cgggcagaat tggcttgatg
    39661 atctcttcca ttaccgcttc ttgcagcgat ttctggtcga tctcttcaga atgctgagtg
    39721 gaaagcacga cagcatcgat accaacgatt ttgccgtcgt catactggaa agtcacctgg
    39781 cttttcgcgt ccgggcgcag ccacggcaga gtgccgtttt tacgcacttc agcctgacgc
    39841 tgtaccagac ggtgtgcata ggtgataggt gctggcatca gcacgtcggt ttcattagtt
    39901 gcgtagccaa acatcagacc ctggtcaccc gcgccctgtt ccagcggatc ggcacggtca
    39961 acgccctggt tgatgtcagg agactgtttg ccgatagcgc tcagaaccgc acaggagtta
    40021 gcgtcaaagc ccatgtcgga atgcacatag ccaatttcgc gaacggtgtt acgggtgatc
    40081 tcttcgatgt ctacccaggc gctggtggtg atttcgccgc caactaaaac catgccggtt
    40141 tttacgtagg tttcgcaagc aacgcgtgct ttcggatcct gttcgaggat cgcgtctaaa
    40201 acggcatcag agatttggtc agcaattttg tcaggatgcc cttcagagac ggactcggac
    40261 gtaaaaaggt gttttgccat atttaatatc acctaaagag aatttggtta gctcaaactg
    40321 ttgtgtggat tttctgtggt agcggatcct accacgactc tgcaggttaa aaacactggc
    40381 agtctgagtg ttaatcggta tggatggatt aacatctgga tggctatttt aggtcaattc
    40441 ttcacccgat ttccactttt ttttgaatcg tgtctcattc tgttaaaaac gtggctggaa
    40501 atttttcctg acaatgccgg cattctgcgt atttatcttt tgcaattttc tgccattgtg
    40561 gggtataaaa cgcggcgcgc ggcttaaata aaaagcacac gacgtttctt tcgtgttgcc
    40621 acttccagcc gggttgaaat cagagttttg gcttgtgggt tcgtcttaac aggcggccgt
    40681 ggaggtgata cgaaataatg aaccgttgtc tgctgcttaa cctgtctcac cgttctggtg
    40741 aagattcgtt ccccgcactc tgcatctctg ctttgcatac ctgccgatgt tatacccatc
    40801 tcggcgcttc tcaggattca agagctggtt acagttactg aggactgaac aagggcgctc
    40861 ttgtaaaaac aagagttttc tcgtggtttc gccgaactgt cacacttacg ttcggttatg
    40921 tgcttaataa tgttatgaaa aagaaaccgg ttgcgcagtt ggagcgtcag cattcactgc
    40981 tggaaaatcc atgtgcttat gggttgttat cgcagttcca ggctgcgata gtcgttaact
    41041 gttttacact taataaaata atttgaggtt cgctatgtct gacgacatgt ctatgggttt
    41101 gccttcgtca gcgggcgaac acggtgtact acgctccatg caggaggttg caatgagctc
    41161 ccaggaagcc agcaagatgc tgcgtactta caatattgcc tggtggggca ataactacta
    41221 tgacgttaac gagctgggcc acatcagcgt gtgcccggac ccggacgtcc cggaagctcg
    41281 cgtcgatctc gcgcagttag tgaaaactcg tgaagcacag ggccagcgtc tgcctgcact
    41341 gttctgtttc ccacagatcc tgcagcaccg tttgcgttcc attaacgccg cgttcaaacg
    41401 tgcgcgggaa tcctacggct ataacggcga ttacttcctt gtttatccga tcaaagttaa
    41461 ccagcaccgt cgcgtgattg agtccctgat tcattcgggc gaaccgctgg gtctggaagc
    41521 cggttccaaa gccgagttga tggcagtgct ggcacatgct ggcatgaccc gtagcgtcat
    41581 cgtctgcaac ggttataaag accgcgaata tatccgcctg gcattaattg gcgagaagat
    41641 ggggcacaag gtctatctgg tcattgagaa gatgtcagaa atcgccattg tgctggatga
    41701 agcagaacgt ctgaatgtcg ttcctcgtct gggcgtgcgt gcacgtctgg cttcgcaggg
    41761 ctcgggtaaa tggcagtcct ccggcgggga aaaatcgaag ttcggcctgg ctgcgactca
    41821 ggtactgcaa ctggttgaaa ccctgcgtga agccgggcgt ctcgacagcc tgcaactact
    41881 gcacttccac ctcggttcgc agatggcgaa tattcgcgat atcgccacag gcgttcgtga
    41941 atcggcgcgt ttctatgtgg aactgcacaa gctgggcgtc aatattcagt gcttcgacgt
    42001 cggcggcggt ctgggcgtgg actatgaagg cacccgttcg cagtctgact gctccgtaaa
    42061 ctacggcctc aatgaatatg ccaacaacat catctgggcg attggtgatg cgtgtgaaga
    42121 aaacggtctg ccgcatccga cggtaatcac cgaatcgggt cgtgcggtga ctgcgcatca
    42181 caccgtgctg gtgtctaata tcatcggcgt ggaacgtaac gaatacacgg tgccgaccgc
    42241 gcctgcagaa gatgcgccgc gcgcgctgca aagcatgtgg gaaacctggc aggagatgca
    42301 cgaaccggga actcgccgtt ctctgcgtga atggttacac gacagtcaga tggatctgca
    42361 cgacattcat atcggctact cttccggcac ctttagcctg caagaacgtg catgggcaga
    42421 acaactctat ctgagcatgt gccatgaagt gcagaagcaa ctggatccgc aaaaccgtgc
    42481 gcatcgtccg ataatcgacg aactgcagga acgtatggcg gacaaaatgt acgtcaattt
    42541 ctcgctgttc cagtcaatgc cggacgcatg ggggatcgac cagttgttcc cggttctgcc
    42601 gctggaaggg ctggatcaag tgccggaacg tcgcgctgtg ctgctggata ttacctgtga
    42661 ctctgacggt gctatcgacc actatattga tggtgatggt attgccacga caatgccaat
    42721 gccggagtac gatccagaga atccgccgat gctcggtttc tttatggtcg gcgcatatca
    42781 ggagatcctt ggcaatatgc acaacctgtt cggtgatacc gaagcggttg acgtgttcgt
    42841 gttccctgac ggtagcgtag aagtagaact gtctgacgaa ggcgataccg tggcggacat
    42901 gctgcaatat gtacagctcg atccgaaaac gctgttaacc cagttccgcg atcaagtgaa
    42961 gaaaaccgat cttgatgctg aactgcaaca acagttcctt gaagagttcg aggcaggttt
    43021 gtacggttat acctatcttg aagatgaata attaagatta ttccgctggc gggtcgattt
    43081 atttacgtaa atcggcccgc tttcttttta tatattctta aaaataatct cgctgctttt
    43141 cttattaaat aatctcccta caatcgctgc attaattaaa tgcagaggga acggttatga
    43201 aaaaatggaa agtacgtagc gcgttggtgg cattaatcgt gctgctggcc ggttgttcca
    43261 gtaatgcgca gtataatagc agtgcatcgg gcaatgtcgg cactgcctgg ggtggggatg
    43321 tgcactcatc ggttcagggc gtatctgctg agcgagcctg gcgcgatccg gcagagatga
    43381 tcgttattag ttattcgact aatgttcctt ccggctatga ccgcgtttat agcattcgta
    43441 ttaacgaact ggaatatgct attcgcgatg gtaattttaa ttctctacct attacgcgtg
    43501 tttatgattc atccaataat gaaccacgct atattgtcca tgccagagtc ggaatgaatt
    43561 atcaattata tgtccgtaat tacagtcgta acaccaacta cgagatcgtt gctactgttg
    43621 atggcctgga tgtgctgaac ggcaagcaag gttcgctgaa taacaacggc tatattgtga
    43681 atgccggaga ttctttagcg attaaaggat tccgcaaaga taaacatacc gaagcggctt
    43741 ttcagttcgc caacgttgct gattcctacg cagccaattc ggcgcaaggc gatgtgcgta
    43801 ataccggtgt aataggcttt gccgcctttg aattacaagg ccaggcgcaa aacgcgctgc
    43861 cgccgtgctc tggacaagcc tttccggcag ataacaatgg ctatgcgccg ccaccgtgtc
    43921 ggaaatagca acctcgcttg atgcctcgcc catttaaggc gataattagc acaaacttgt
    43981 gtttcgcaaa tccaaccctt cctcgtcggg cctaacgacg cggaagggtt tttttatatc
    44041 gactttgtaa taggagtcca tccatgagca ccttaggtca tcaatacgat aactcactgg
    44101 tttccaatgc ctttggtttt ttacgcctgc cgatgaactt ccagccgtat gacagcgatg
    44161 ctgactgggt gattactggt gtgccgttcg atatggccac ttctggtcgt gcggggggac
    44221 gccacggtcc ggcagctatc cgtcaggttt cgacgaatct ggcctgggaa cacaaccgct
    44281 tcccgtggaa tttcgacatg cgtgagcgtc tgaacgtcgt ggactgcggt gatctggtat
    44341 atgctttcgg cgatgcccgt gagatgagcg agaagctgca ggcgcacgcc gagaagctgc
    44401 tggctgccgg taagcgtatg ctctccttcg gtggtgacca ctttgttacg ctgccgctgc
    44461 tgcgtgctca tgcgaagcat ttcggcaaaa tggcgctggt acactttgac gcccacaccg
    44521 atacctacgc gaacggttgt gaatttgacc acggcaccat gttctatacc gcgccgaaag
    44581 aaggtctgat cgatccgaat cattccgtgc agattggtat tcgtaccgaa tttgataaag
    44641 acaacggctt taccgtgctg gacgcctgcc aggtgaacga tcgcagcgtg gatgacgtta
    44701 tcgcccaggt gaaacaaatt gtgggtgata tgccggttta cctgaccttt gatatcgact
    44761 gcctggatcc tgcatttgcg ccgggaaccg gtacgccggt aattggcggt ctgacctccg
    44821 atcgcgccat taaactggta cgcggcctga aagatctcaa catcgttggg atggacgtag
    44881 tggaagtggc tccggcatac gatcagtcgg aaatcactgc tctggctgcg gcgacgctgg
    44941 cgctggagat gctgtatatt caggcggcga aaaagggcga gtaagcacca gatgcgatgc
    45001 gcacgggtaa aacgtgccat taatgtcgga tgcggcgtga acgccttatc cgacctacgt
    45061 tcggcacccg taggccggat aagatgcgcc agcatcgcat ccggcaatgc gcacaaggta
    45121 acaaatgtgc cattcatgtc agatgcgggg tgaacgcctt atctgaccta cgttcgacac
    45181 caccaggctt tactttatcc catccgcgct catacgatcg cgaatatgct gggcgcgttc
    45241 ggcggatgca ggatggtcgt caaacattga gctttggcgg ccttcttcca gtttcgccag
    45301 tttttcaaag ctggtggcaa gacctgccgg gctgatgccg cgttggcgca gaagatcgta
    45361 agaataatca tcggcttctg cttcctggcg ctgggagaat tgcgaattaa ctaatttctc
    45421 gcccagatca ccgagttgtg attgagataa actgccgaca atcccgcccg cagaggccgc
    45481 agctactcgc acggcatttg taccaagtgc cacctgcatt ccttttttca catggcctaa
    45541 cgccacgtgc cccatttcgt gaccgatcac cgcttcgact tcgttatccg tcatcatatc
    45601 catcagcccg ctatagacgc ggatacagcc gttagccatt gcaaaggcgt tcacatcctt
    45661 cgccatatac actttgtaat ttaccggctg accgttgata ttgttgccta gcgcattggc
    45721 aatagttgtc agacgtttag cgtattcgct attggctggc gcaatcgtcg ccttgctgtc
    45781 catctcctga catgcctgat cgctcagggt tttcacctgc gcatcactca aactgtaagc
    45841 ctgaaaagct tccgctcctg atgagagcag tccgttggag tccatattct ggcaaccagt
    45901 cagtaccgtt gccacgctca ttgctaccaa taaggcgcga attttcatgt tttctcttcc
    45961 gcacaaggtc aggattattc taaaaatgcg ccgtttgcag gtgaatcgac gctcagtctc
    46021 agtataagga aaagcgcagc ggacgggcga gtagattgcg caacatgcga gcatgatcca
    46081 gagatttctg aagcggcaaa aggatgttcc atgtacatga cgcgcggctt gcggtaaatt
    46141 gttggcaaat tttccggcgt agcccaaaac gcgctgtcgt caagtcgtta agggcgtgcc
    46201 cttcatcatc cgatctggag tcaaaatgtc ctcacgtaaa gagcttgcca atgctattcg
    46261 tgcgctgagc atggacgcag tacagaaagc caaatccggt cacccgggtg cccctatggg
    46321 tatggctgac attgccgaag tcctgtggcg tgatttcctg aaacacaacc cgcagaatcc
    46381 gtcctgggct gaccgtgacc gcttcgtgct gtccaacggc cacggctcca tgctgatcta
    46441 cagcctgctg cacctcaccg gttacgatct gccgatggaa gaactgaaaa acttccgtca
    46501 gctgcactct aaaactccgg gtcacccgga agtgggttac accgctggtg tggaaaccac
    46561 caccggtccg ctgggtcagg gtattgccaa cgcagtcggt atggcgattg cagaaaaaac
    46621 gctggcggcg cagtttaacc gtccgggcca cgacattgtc gaccactaca cctatgcctt
    46681 catgggcgac ggctgcatga tggaaggcat ctcccacgaa gtttgctctc tggcgggtac
    46741 gctgaagctg ggtaaactga ttgcgttcta cgatgacaac ggtatctcta ttgatggtca
    46801 cgttgaaggc tggttcaccg acgacaccgc aatgcgtttc gaagcttacg gctggcacgt
    46861 tattcgcgac atcgacggtc atgacgcggc atccatcaaa cgcgcagtag aagaagcgcg
    46921 cgcagtgact gacaaaccgt ccctgctgat gtgcaaaacc atcatcggtt tcggttcccc
    46981 gaacaaagcc ggtactcacg actcccacgg tgcgccgctg ggcgacgctg aaattgccct
    47041 gacccgcgaa cagctgggct ggaaatatgc accgttcgaa atcccgtctg aaatctatgc
    47101 tcagtgggat gcgaaagaag caggccaggc gaaagaatcc gcatggaacg agaaattcgc
    47161 tgcttacgcg aaagcttatc cgcaggaagc cgctgaattt acccgccgta tgaaaggcga
    47221 aatgccgtct gacttcgacg cgaaagcaaa agagttcatt gctaaactgc aggctaaccc
    47281 ggcgaaaatc gccagccgta aagcgtctca gaatgctatc gaagctttcg gtccgctgtt
    47341 gccggaattc ctcggcggtt ctgctgacct ggcaccgtct aacctgaccc tgtggtctgg
    47401 ttctaaagca atcaacgaag atgctgcggg taactacatc cactacggtg ttcgcgagtt
    47461 cggtatgacc gcgattgcta acggtatctc cctgcacggt ggtttcctgc cgtacacctc
    47521 caccttcctg atgttcgtgg aatacgcacg taacgccgta cgtatggctg cgctgatgaa
    47581 acagcgtcag gtgatggttt acacccacga ctccatcggt ctgggcgaag atggcccgac
    47641 tcaccagccg gtagagcagg tcgcttctct gcgcgtaacc ccgaacatgt ctacatggcg
    47701 tccgtgtgac caggttgaat ccgcggtcgc gtggaaatac ggtgttgagc gtcaggacgg
    47761 cccgaccgca ctgatcctct cccgtcagaa cctggcgcag caggaacgaa ctgaagagca
    47821 actggcaaac atcgcgcgcg gtggttatgt gctgaaagac tgcgccggtc agccggaact
    47881 gattttcatc gctaccggtt cagaagttga actggctgtt gctgcctacg aaaaactgac
    47941 tgccgaaggc gtgaaagcgc gcgtggtgtc catgccgtct accgacgcat ttgacaagca
    48001 ggatgctgct taccgtgaat ccgtactgcc gaaagcggtt actgcacgcg ttgctgtaga
    48061 agcgggtatt gctgactact ggtacaagta tgttggcctg aacggtgcta tcgtcggtat
    48121 gaccaccttc ggtgaatctg ctccggcaga gctgctgttt gaagagttcg gcttcactgt
    48181 tgataacgtt gttgcgaaag caaaagaact gctgtaatta gcatttcggg taaaaaggtc
    48241 gcttcggcga ccttttttat taccttgata tgtccgtttg cggacaagca atagataaat
    48301 cgtgttgtag atcacaaata tttatatgca ataaatatca att
//
LOCUS       DAPGWD010000019        47761 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPGWD010000019 DAPGWD010000000
VERSION     DAPGWD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.019"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(120..2054)
                     /gene="rnb"
                     /locus_tag="RHX88_001019"
     CDS             complement(120..2054)
                     /gene="rnb"
                     /locus_tag="RHX88_001019"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDV2229744.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(2122..3249)
                     /locus_tag="RHX88_001020"
     CDS             complement(2122..3249)
                     /locus_tag="RHX88_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDV2229745.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(3394..4182)
                     /gene="fabI"
                     /locus_tag="RHX88_001021"
     CDS             complement(3394..4182)
                     /gene="fabI"
                     /locus_tag="RHX88_001021"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDV2229746.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(4550..4903)
                     /locus_tag="RHX88_001022"
     CDS             complement(4550..4903)
                     /locus_tag="RHX88_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDV2229747.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(4971..5777)
                     /gene="sapF"
                     /locus_tag="RHX88_001023"
     CDS             complement(4971..5777)
                     /gene="sapF"
                     /locus_tag="RHX88_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDV2229748.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(5779..6771)
                     /gene="sapD"
                     /locus_tag="RHX88_001024"
     CDS             complement(5779..6771)
                     /gene="sapD"
                     /locus_tag="RHX88_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDV2229749.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(6771..7661)
                     /gene="sapC"
                     /locus_tag="RHX88_001025"
     CDS             complement(6771..7661)
                     /gene="sapC"
                     /locus_tag="RHX88_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDV2229750.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(7648..8613)
                     /gene="sapB"
                     /locus_tag="RHX88_001026"
     CDS             complement(7648..8613)
                     /gene="sapB"
                     /locus_tag="RHX88_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDV2229751.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(8610..10253)
                     /gene="sapA"
                     /locus_tag="RHX88_001027"
     CDS             complement(8610..10253)
                     /gene="sapA"
                     /locus_tag="RHX88_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDV2229752.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(10566..10811)
                     /locus_tag="RHX88_001028"
     CDS             complement(10566..10811)
                     /locus_tag="RHX88_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDV2229753.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(10945..12330)
                     /gene="puuP"
                     /locus_tag="RHX88_001029"
     CDS             complement(10945..12330)
                     /gene="puuP"
                     /locus_tag="RHX88_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDV2229754.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(12320..12484)
                     /locus_tag="RHX88_001030"
     CDS             complement(12320..12484)
                     /locus_tag="RHX88_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229755.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            complement(12633..14051)
                     /gene="puuA"
                     /locus_tag="RHX88_001031"
     CDS             complement(12633..14051)
                     /gene="puuA"
                     /locus_tag="RHX88_001031"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDV2229756.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            14263..15027
                     /gene="puuD"
                     /locus_tag="RHX88_001032"
     CDS             14263..15027
                     /gene="puuD"
                     /locus_tag="RHX88_001032"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDV2229757.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            15054..15611
                     /gene="puuR"
                     /locus_tag="RHX88_001033"
     CDS             15054..15611
                     /gene="puuR"
                     /locus_tag="RHX88_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDV2229758.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            15886..17373
                     /gene="puuC"
                     /locus_tag="RHX88_001034"
     CDS             15886..17373
                     /gene="puuC"
                     /locus_tag="RHX88_001034"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDV2229759.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            17375..18655
                     /gene="puuB"
                     /locus_tag="RHX88_001035"
     CDS             17375..18655
                     /gene="puuB"
                     /locus_tag="RHX88_001035"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDV2229760.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            18693..19958
                     /gene="puuE"
                     /locus_tag="RHX88_001036"
     CDS             18693..19958
                     /gene="puuE"
                     /locus_tag="RHX88_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDV2229761.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPELVEAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYVDKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(20078..21055)
                     /gene="pspF"
                     /locus_tag="RHX88_001037"
     CDS             complement(20078..21055)
                     /gene="pspF"
                     /locus_tag="RHX88_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDV2229762.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            21222..21890
                     /gene="pspA"
                     /locus_tag="RHX88_001038"
     CDS             21222..21890
                     /gene="pspA"
                     /locus_tag="RHX88_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDV2229763.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            21944..22168
                     /gene="pspB"
                     /locus_tag="RHX88_001039"
     CDS             21944..22168
                     /gene="pspB"
                     /locus_tag="RHX88_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDV2229764.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            22168..22527
                     /gene="pspC"
                     /locus_tag="RHX88_001040"
     CDS             22168..22527
                     /gene="pspC"
                     /locus_tag="RHX88_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDV2229765.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            22536..22757
                     /gene="pspD"
                     /locus_tag="RHX88_001041"
     CDS             22536..22757
                     /gene="pspD"
                     /locus_tag="RHX88_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDV2229766.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            22832..23146
                     /gene="pspE"
                     /locus_tag="RHX88_001042"
     CDS             22832..23146
                     /gene="pspE"
                     /locus_tag="RHX88_001042"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDV2229767.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            23358..25037
                     /gene="ycjM"
                     /locus_tag="RHX88_001043"
     CDS             23358..25037
                     /gene="ycjM"
                     /locus_tag="RHX88_001043"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDV2229768.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            25051..26343
                     /locus_tag="RHX88_001044"
     CDS             25051..26343
                     /locus_tag="RHX88_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2229769.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            26364..27245
                     /locus_tag="RHX88_001045"
     CDS             26364..27245
                     /locus_tag="RHX88_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDV2229770.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            27232..28074
                     /locus_tag="RHX88_001046"
     CDS             27232..28074
                     /locus_tag="RHX88_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDV2229771.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            28105..29157
                     /locus_tag="RHX88_001047"
     CDS             28105..29157
                     /locus_tag="RHX88_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDV2229772.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVD
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            29175..29963
                     /locus_tag="RHX88_001048"
     CDS             29175..29963
                     /locus_tag="RHX88_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV2229773.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLNGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            29973..31028
                     /locus_tag="RHX88_001049"
     CDS             29973..31028
                     /locus_tag="RHX88_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV2229774.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIRQQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            31025..33292
                     /gene="ycjT"
                     /locus_tag="RHX88_001050"
     CDS             31025..33292
                     /gene="ycjT"
                     /locus_tag="RHX88_001050"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDV2229775.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHETWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRIAVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPYNGTATKHQEDE"
     gene            33289..33948
                     /gene="pgmB"
                     /locus_tag="RHX88_001051"
     CDS             33289..33948
                     /gene="pgmB"
                     /locus_tag="RHX88_001051"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDV2229776.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRCILQHGGKEGDFNSQERAQLAYRKNLLYVHSLCELTVNAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            33962..35044
                     /locus_tag="RHX88_001052"
     CDS             33962..35044
                     /locus_tag="RHX88_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2229777.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            35089..35994
                     /gene="ompG"
                     /locus_tag="RHX88_001053"
     CDS             35089..35994
                     /gene="ompG"
                     /locus_tag="RHX88_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDV2229778.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(36105..37103)
                     /locus_tag="RHX88_001054"
     CDS             complement(36105..37103)
                     /locus_tag="RHX88_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2229779.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            37258..38655
                     /locus_tag="RHX88_001055"
     CDS             37258..38655
                     /locus_tag="RHX88_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDV2229780.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            38652..39713
                     /locus_tag="RHX88_001056"
     CDS             38652..39713
                     /locus_tag="RHX88_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDV2229781.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            39861..41402
                     /gene="tyrR"
                     /locus_tag="RHX88_001057"
     CDS             39861..41402
                     /gene="tyrR"
                     /locus_tag="RHX88_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDV2229782.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(41446..41952)
                     /gene="tpx"
                     /locus_tag="RHX88_001058"
     CDS             complement(41446..41952)
                     /gene="tpx"
                     /locus_tag="RHX88_001058"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDV2229783.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            42071..43036
                     /gene="ycjG"
                     /locus_tag="RHX88_001059"
     CDS             42071..43036
                     /gene="ycjG"
                     /locus_tag="RHX88_001059"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDV2229784.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(43011..43739)
                     /gene="mpaA"
                     /locus_tag="RHX88_001060"
     CDS             complement(43011..43739)
                     /gene="mpaA"
                     /locus_tag="RHX88_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDV2229785.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLSQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(44030..44668)
                     /locus_tag="RHX88_001061"
     CDS             complement(44030..44668)
                     /locus_tag="RHX88_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2229786.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(44686..45618)
                     /locus_tag="RHX88_001062"
     CDS             complement(44686..45618)
                     /locus_tag="RHX88_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2229787.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKSHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            complement(45618..46538)
                     /locus_tag="RHX88_001063"
     CDS             complement(45618..46538)
                     /locus_tag="RHX88_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2229788.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            46676..47575
                     /gene="pgrR"
                     /locus_tag="RHX88_001064"
     CDS             46676..47575
                     /gene="pgrR"
                     /locus_tag="RHX88_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDV2229789.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT        11728 a        11990 c        12695 g        11348 t
ORIGIN      
        1 cagttttgga aaaacgccag ttttcaaacg aaagtcagtt aaaaaatctg cctggatata
       61 acgaaggtag agcggggaaa taaacggccc atccatgagg aatgggccgt gaaaggagat
      121 tacgcgaccg ggcgcgcaat aatgctgcgg gtttccatgc ggacttcggc aatggtgacg
      181 tcaataacgt cagttacttt gtaaaccgtt tcacctttaa tttgtacagt gccgttttcc
      241 tggctgcaaa ccagttcatc gcgcatagcg tgtaagaaag gtgccggaat aaaggcgata
      301 gcgccgttat caaccaaacg cacacgcatg ccgccacggc tgatatcgac aatttccgcc
      361 gcgaaacggg tgtcggtccc ggctttgtct ttcaggaagc gtgcgtataa ccagtcgcca
      421 acatcacgtt ctgccatccg gttgagacgg cgacgctcgg ccatttggac agtgatttca
      481 tcctgtgggc gcgtcgcagt ttcgcctttg ataaccgctt tcagcagacg gtggttgatc
      541 atgtcgccat atttacggat cggcgaagtc caggtggcgt atgcttccag accgaggcca
      601 aagtgaggac cgggttcagt gctaatttca gcaaatgact ggaagcgacg aatgcggctg
      661 tcgaggaaac cagttggttg cgcatccagt tcacgacgca gtttgcagaa accgtccagc
      721 gtgagcactt cttcggcatc gacatgcaga ccgtgcgttt tcagcaacgc tgccagcgcg
      781 tcggcattcg ccggatcaaa gcccatatgc acgttataga tgccaaaacc gagcttatcg
      841 cgcagtacgc gggccgcaca aatgttagcg gcaatcatcg cttcttcgac gatacggttg
      901 gcaatgcgac gaggctcggc gacgatatcc agcacttcac ctttttcacc gagaataaag
      961 cggtaatccg ggcgatcttt aaacaccagt gcgtggttat gacgccactc gccgcggcgt
     1021 tggcaaattt gcgctagcaa acggacttgt tcggcaattg cttcactttc aggctgccag
     1081 tcaccggtat tttccagcca gtcagaaacc tggtcataca ccagctttgc tttggattcg
     1141 atggtggcgg caaagaattc gatattatct tcaatggtgc catcagcgga gagcgtcatg
     1201 cggcatgcca gtaccgggcg gacttcattg gcgcgcagtg agcagagatc gtcagaaagc
     1261 tcgcgaggca gcatagggat gttgaagcca ggcagatagt tggtgaatgc gcgaattttc
     1321 gcggctttgt ccagcttgct gccttcagca atccacgcgg ttggatcggc aatcgccaca
     1381 atcagctgaa gtttgtcatc cggcaacgcc ttagcgaaaa gggcgtcatc catatcttct
     1441 gtgctggcac tgtcgatggt gacaaaatcc agcgcggtca gatcttcacg aaccagacct
     1501 tcatcgagca tttcggtagc gacgccgtct ggtgcttctt tttccagatt atggcgcgca
     1561 agggtaaccc accacggtac aaagtggtcg tcaccaaaag tgatgtattg tgtcagttca
     1621 gcatagaaag aacgatcgcc tttcagcgga tgacggcgca tttcggcaac cgcccagtcg
     1681 ccttctttaa actcgtggtt caggccacgg gctgcgcggc aaggaatggc gtcttttaag
     1741 agtggatgat caggaacgat ggccagacgg tcatttttgc cctgaacttt acccacgaaa
     1801 cgagtcagga acggttcaac cagttcttct ggctctgcgg attcacgttc tttttcactg
     1861 tggatcaccg cgataattcg gtcgccatgc atgacttttt tcatctgcgg cggcggaatg
     1921 aaataacttt tttgcgcgtc gacttccagg aagccaaagc ctttttctgt ggcttttacc
     1981 accccttcag cgcgtggcgt ctgggaatgc agttgctgtt taagctgcgc tagcagcggg
     2041 ttgtcctgaa acataattgt ctattttggt ggccattaga gcggctgaca gttttacgcg
     2101 aatctgtctg acgcggcaag gttaatatgt ctcacccaac gcgattttta agcgatttat
     2161 ccagccacac agcccgctcc ataccagcag attaataatc tgcgttgatg atttttcgtg
     2221 ttcgagtaaa gggctgaact gggcggcgct aaagcgatcg ggtgctcggg tcagccattg
     2281 tatcgcctga acggtagcgt gctcaacaga atgacggtca gcccattggt taatttcatt
     2341 ttcgcccttg cgcagaaggg ggctgatttt tttatattca tttgatgaat ccatacaggc
     2401 ggcaaaacaa gcgggactgc catttatccg cgcgcataac aacgccgcaa gcttattatc
     2461 cggctgtgta ttaacccgtg tactgtttaa caacctttcg agaagggaaa gtgaaatagg
     2521 gtggagagag agtgtttcgg ccagttggca aagttctgct gttgaacttt ttgtgtactc
     2581 ttcaggtagt ttttccacct catagctgct tcgccagcgt attgattcat cagcaaacag
     2641 tgacgcgtcg gcgtagtttt gtatttccag accgggtaac cagcgtaccg ggtgcccgag
     2701 ataagcctga aatgtcgcaa tggtccgcgc ctgaaagcca ataaaaccaa tgatttggtt
     2761 aatgatcaca cagtccccgg cagtaagacc gacgtcattc agatgctggc gtgatcggtt
     2821 gtcgataata gaaggcgagc tagccagttg gcgggcatat tgcgtgattt gtgccaacct
     2881 atgattgctt tccctggagg aatcaggccc ggggagcggc gtaagtcgcg ccgaatagta
     2941 gttacataac cgctggacgc cgcaaacctg agcaaccgtc agggccgtag acaagcgttc
     3001 ataagcggtg aaggtctgtg aacgggtcac ggtaagctgg tcagggaaca attccactgc
     3061 aagaacgcgc gcagggacca gccatgaaag gaagggagcc agcgtttctt catcaaggat
     3121 cacgtcgagc agaaagggat cgctgacctt tgcggcttcc gggaccagag gcagaacgtc
     3181 atactccgca gtactggatt gcgtttcatg ataccagtgg cttttgccgg tgatgtggcg
     3241 ttgttccatg ggcgctcctt ggtcgtaaag gaaatcgtta tcctgacgca aggcgggaag
     3301 gggagaaaga cggatcggcg ataacaaata tcagaaaggt ataacagaga taacgggcgg
     3361 cagaacgccg cccaatattt accaacagaa tgattatttc aattcgagtt cgttcattgc
     3421 agcgatgctg aaaccgccgt caacgtggac cacttcaccg gagataccgg cagagagatc
     3481 ggagcacagg aatgccgcgg agttacccac atcttcaata gtaacggtac ggcgaatcgg
     3541 ggtaacggct tcgcaatgag ccagcatttt gcggaagtct ttgataccgg aggccgccag
     3601 agtacggatc ggaccagcag agatggcgtt aacacgcaca ccttccggac ccatcgcgtt
     3661 cgccatatag cgcacgttcg cttccagaga cgcttttgcc agacccataa cgttgtagtt
     3721 cgggatagcg cgctcagcgc caaggtagga aagggtcagc agggcagaac ccggattcag
     3781 catggagcgg caagcttttg ccattgcaac gaagctgtag gagctgatgt cgtgggcaat
     3841 tttgaagcct tcacgggtaa cggcgttaac atagtcacca tccagctgat cgccaggtgc
     3901 aaaaccaata gagtgtacga aaccgtcaaa tttcggccaa actttcccca gttcagcgaa
     3961 catggtgtcg atgctggcat cttctgcaac atcgcactgc agaacgatgt cagaacccaa
     4021 ttgagcggca aattcttcta cgcggccttt cagtttgtcg ttctggtagg tgaatgccag
     4081 ttcagctcct tcgcggtgca tcgcctgagc gataccgtag gcgatggata gtttgctggc
     4141 aacaccggtt accagaatgc gcttaccgga aagaaaaccc atagctttaa tccttattgt
     4201 tgatgcttgt tgtgcctgaa aatcaggcga ttcgtcgttt tagtaaacag tacgaacaga
     4261 taaacggtta ttataatcaa cctggctgtg agtagctata gttgccaggt ccgaccggag
     4321 caggctgcgg cagggtgggc gcttttcccc ttaccctaac cctctcccca gaggggcgag
     4381 gggaccatat tgtgcaaata ttgttacccc agcaacaaac aggctcatac agcccctaac
     4441 ccttttatgg cgatggctgg gatggttcag accttgccga atattctcca gcactgtctc
     4501 catgttttca ccacaacagc tattcggctc ggtcggcccc ctcgcccttt cagggtgagg
     4561 gtgaccggcg gttcagttcc tgcagaataa tctccagcac cgcctcctca ttacaatcaa
     4621 tctcgttatg ccagaaacgc agcacggtcc agctctgcga ctcaagccag ctagtgcgcc
     4681 tggtatcata ggcaactgcc aaatcatgct gcccaccatc cagctcaacg actacgcgcg
     4741 ccgagcagca agcaaaatcg agaatgtagc tccccaccgg atgttgacgg cgaaatttga
     4801 aatcaccaaa acgtcggctg cgaagatatc gccagagctt tcgttcctgc aaagtgagat
     4861 tgcgtcttaa atcacgcgca tttgatttta ttttatccat cacctcattc tgacaagatt
     4921 taatctcttg tcagaatgag gtggataatc tggaaggagg attcagaaaa ttagcgatct
     4981 ttacgccacg catccgctgt caatgcttcg ccaaagtgcc cggcaatcag ccgtttggtg
     5041 agttcatgca gcggcgatgc cagcacatcc gcggtgctgc ctcgctcgac aacctcgccc
     5101 tgatgcatca ccagcacctg gtcgctaatg tgcttcatca ttccgatatg ctgggtaaca
     5161 taaatatacg aaatgccctg tttttcctgt aattccagca tcagattaat caactgcgaa
     5221 cgcatcgaca tatccagtga agcgagggct tcatcggcaa taatgacttt tgggcgcaat
     5281 atcagcgcgc gcgccagacc cagacgctgt ttttgtccgg gtgccaacat atgcggatag
     5341 taactgacgt gatccggtag cagcccaacc atacgcatcg tttcaataat ctgtttgcga
     5401 cgctgttccg gttccaggtc ggtgttcagg cgcagtggaa aatccagaat ttgcgagata
     5461 cgttgacggg gattcaacga ggtcgaagga tcctgaaaaa tcatgcgaat acgctgactg
     5521 cggaaggaat aatcgccaaa atgcagtgga tgatcgtcaa tcaataactc gccgctggta
     5581 ggctctatca ttcccgccag catttttgcc agcgtggatt tacccgaacc attctcgcca
     5641 ataatcgcca gtgtctggcc ttcacgtagc gtaaagctca agggttttac cgcttctacg
     5701 gtctgacgac gaaaccagcc ggtccggtag cggaacgttt tacttaaatt acgcacttca
     5761 agcagcgttt cgatcatctc actctttctc catgttcagc gggaaatgac aggcatagag
     5821 atgatttttc gcccccgtca aacgtggcgt cacaatgcat tctcgttgtg catacgggca
     5881 acgtggcccc agacgacaac caatcggtaa ctgttccagc agtgggatag cgccgggcag
     5941 cgtattgagg cgacttttat gcggcatcgc gctgccgaag tctggtatcg cgcggatcag
     6001 cgcctgggta taaggatgat gtggcatcgt caccaactcc ttactcggcg cggtttccac
     6061 tgtttgaccg cagtaaagca cgttaatttt atccgcccat tggctaagca tttgtaagtc
     6121 atggctgata agcaaaatag tggtattgct gttttggttg agacgcgtca gcaggcgaaa
     6181 gatttgcgcc tgggttgttg gctccattga gttggtcggt tcgtcagcaa tcagcagacg
     6241 cggttgattc gccagtgcaa tggctatcat cactttctga cattcacctt cggtcaactc
     6301 atagggaaaa ctgcgcatcg catctttgtg atctttaatc cccacgcggt gcagcagttc
     6361 aatggcacgg cgtttgcgcc agccaaaacg ctgccaccaa cggcctttat aggtccaggc
     6421 tgggatgttt tgcatcaact ggcgacccac acgttctgaa gggtcaagac acgactgcgg
     6481 ttcctggaaa atcatcgaca cgttatggcc aaccagtttg cgccgttcgc gtgcggagag
     6541 acgcagcaaa tcgatatcat caaaacgcat acggtcagca gtaacacgcc agttatcttt
     6601 attcacccca caaatcgctt tcgcaatcaa actcttgccg gaaccggatt caccaacaag
     6661 accgcggatt tcaccttcgg ttaaggtcat acttacccgg tcgacggctt taacccactc
     6721 atcaccggtt ttaaattcaa tggtcaggtt acgaatatcg agtaatggca ttattccacc
     6781 cccgcaataa tcgcacgacg aacgccatcg ccaaggaggt taaccaacaa cacgctaatc
     6841 ataattgccg cacctggcag catgacagtc cacggggcga cataaatcag ttccagcgca
     6901 tcaccgagca tcgctcccca ttcaggcgag gggagttgtg cgccgagatc gagaaagccc
     6961 agcgcggcga tatcgagaat tgccatcgac agtgcgcggg tgatctcggt gaccagcccg
     7021 gcggtgatgt ttggcatcac agcaaaccag agaatattca gcgttgatgc gccatccaga
     7081 cgggcggcga taacgtactc tttttccagt tcgtcatgca ccatgctgta aatcgaacgt
     7141 accatacgcg gcagcaatgc cagccagaca gcaaacatag cgtgggacaa actcggtccg
     7201 gcaaacgcta caacgataat tgccaacagc agcgaaggga tcgccagtaa ggtatccaga
     7261 atatggttaa gcaccgctga gcggaggccg tgtgtcgccc cggcgaaggt gcccagtacc
     7321 aggccacaga tcgtcgcggc aagcgtcacc acaaatgcgc cacccacggt tggcgcagct
     7381 ccgctcagta aacggcttaa aacatcgcgt ccgaggtcgt cagtccccag gaagaaagaa
     7441 acttcgccat agcgcgacca tgacggcggc aataattgat aaccgagaaa ttgctggtcg
     7501 atgccgtaag gcgcaaacca gccgccaaaa atacacagta cagccagccc cgcgcagccg
     7561 tacaggccga ccatcgcaga ggcgtcacta taaaatttgc gccaggcggt acgcagcgtg
     7621 cccggcgggc gcttttcgct gtatacgcta tcgtaaggca taccattcct tatgtttcag
     7681 agggttagcc atggcaccca aaatatcaga aatcacgtta acaataatga ccagtgatcc
     7741 acacaccatc actccggcgg aaatggctgc gtaatcctgc tggcggatgg cgttaattaa
     7801 ccagcgccct aaacccggcc agctaaaaac catttcggtg atcatcgcca gcgtcagcat
     7861 ggtggaaaac tgtaagccca ggcgaggaat aaccggcggt aacgcgttat gcagaacgtg
     7921 gcgacgcaaa atagtaaagc gcgacaaacc gcgggtcgcc gccgctttta catagttctg
     7981 gtcatacact tcgatagtgc tgatacgcat caggcggatc acttcagttg ttggcgcaac
     8041 cgacagggtg atcacgggca atatcatatg gcggattgcg ctcatgatca tttcatcccg
     8101 ccatggtgag tccgaaagcc aggcatcaat caacgcaaaa ccggtaatcg gtttcacttc
     8161 gtagagcaga tcgaaacgcc ctgaaacggg caaccaacct agcgtgagtg aacaaaacag
     8221 cgtcaacaga agcgccagcc agaacaccgg gattgaaaag cccagtaagg cgatggcgtt
     8281 gatcaagtta tcctgccact tatggcgcgt aatcccggca atcatcccca cggggatgcc
     8341 aacaatcagc gcaaaaccga aggcgaggat gcacagttcc atcgtcgccg ggaagacctc
     8401 ttttaactgt tcggcaatcg gttgaccgtt aatgctggaa acaccaaaat cccaatggat
     8461 caggccgtta aaccagaaca cccaggcatt ccacagtgac gcgccttgta acggcgcatg
     8521 aggggtgaaa tagctcaagc tgaagccaac gaaggtcagc aggaacaagg tgacaatcaa
     8581 taacaaaatg cggcgtaagg tgaagataat catggttttt tcacctcatc ctgtttctcg
     8641 cgatacaccc cagcaaagga ggcgttacca aacgggctaa gtaccagacc tttgatatcg
     8701 taccgatagg cctgcaaacg caatgacgac gccagcggca aaatgggcaa ttcctgcgcc
     8761 agaatactct gcgcttcgtc ataggcttca atacgcgccg ccagctgctg cgaagagagc
     8821 gccttacgca atacgctgtc gaatttcgga tcgcaccagt gggcgaggtt ggtctgcgaa
     8881 tgaattgccg cgcagctcag taacggacgg aagaaactgt ccgggtcgtt actgtccgtc
     8941 gcccaaccgg ataacgtcag atcatggctc atatccatca accgcgcctc ctgaaagcga
     9001 ccttctaccg gcacaatcac cacttttacg ccaacctgcg ccatatccgc ctgaatcagt
     9061 tcggcagttt tcagtggact ggggttccac gcctgcgaac gtgtgggcac ccacagtttc
     9121 agcgttaaat tttccagccc caacgacttc aactgttcgc gcgatttcgc cggattgtat
     9181 tcagtaattt tagcctcgtt gtcataggcc cacgaggcgc gcggtaaaat agaggccgcc
     9241 gtttcagccg taccataata gatggattgc atcaggcgct ggttattaat cgccagtgcc
     9301 agcgcatggc ggacagcggg attatttagc ggcggtttgg cggtgttaaa tgccagatag
     9361 gcgacgttca tcccaggacg cagcgttaaa cgcaagcgcg ggtcgtcacg caaaatggat
     9421 agctggctgg cagcaggcca ggccagaacg tcgcattccc cggtcaggag tttcgacaga
     9481 cgtccggtgc cgccggagcc taaatccacc actacctgcg gcattaacgg tttaccgcgc
     9541 cagaagtcat catgacgttg taggcgaata aattgcccgg cgcggtattc cgacaactga
     9601 tacggaccgg tgccgaccgg ttgacggtcg agttgctcct ggcgatcttc tttctctaac
     9661 ttccgggcat attctgccga catgaccgaa gcataatggg ttgcgaggtg ccacaaaaaa
     9721 gaagcatccg gctgggcgag tcggaactca acggtatgat tatccagttt gcggacgctt
     9781 ttcacgttat cggcaaattg caggctgtcg aagtagggga agttgctgcc gttgacgtta
     9841 tgccacgggt tgttgcggtc aaaaattcgc tggaaggtaa acaccacatc gtcggcattc
     9901 attttacgag tgggagtaaa ccagtcggtt ttttgaaacg gaacatcgcg acgcaggtgg
     9961 aagcgatagg tcgcgccgtt gtcgagtact tcccagcttt cggcaagttc cggcatcagg
    10021 cgataggtat agggatcgac atccagcagt cgatcataaa actgggcggc aagggtatcg
    10081 acaattaacc cactgctcgc tttggatggg ttaaaggtgt tgacttgccc gctgacgcaa
    10141 tagacaaaac cgctgtcgcg gatatcagca tgcggggggg attcaggcgc ggcgattgcc
    10201 tgaccactca caagtccagc aatcaccaaa agagacgata atacctggcg cataatatta
    10261 agggatttta tgtaaagagg ctatcttact aatatttaat gacatttgcc attaccgttt
    10321 gtgttcaggg gtcgtaatga gggccgtgtg gttggtcgtt gaacaggtgg cgtcgccatc
    10381 tgttcaacat tcgtacccgt gatttctcta taactataac tcacagaaca acttagcgag
    10441 gagcagagcg gtacgatcgt gagaaaccca ttaaagcacg ctgcagtgcg tgtcattgtt
    10501 agccggatgc ggcgtgaacg ctttatccgg acaacgatac tgaccgatcg tctgcaatta
    10561 aataattact cattacccca ttgattcaaa aactctgcga tctcatcaat gcgtacggga
    10621 ttaatccccg cttcagcagc catttcatgt tgggcttctt cgctgatctc ttcattgttc
    10681 atcaaacggg tgagcagtaa ctggaagtag tgggccagcg gtgtgcgttc agcctctgcg
    10741 acaggctctg cgcattcggt cgcgtactca tcggcaatat caaaatattt aagcgggata
    10801 tcgtggttca ttatttgccc ctggggttaa tgcgctaagc ataagggcgg atgatagcac
    10861 tcgtgatccg ggtcatacat ctttttatag ttaatggcgc ggcgcgttac cctcaggcag
    10921 gataatgcgc cgcgcatccg gctattacgt ttcgctcacc ggcgttctgt caccttcgta
    10981 cagcggcact ttgcgatagc ggcggatcag ataccacaaa tatgcgccgc ctaacgaagc
    11041 ccataccaga ccgagtgtca gtgacgttga ctctaggtta acccacagca cacccaccgt
    11101 cagcgcgcca accagcggca tcagcaaata gtggaagtga tccttccagc ttttattcat
    11161 tcctttacgc cgccagaaat gattaaacac cgacagattc acgaaggtaa acgccaccag
    11221 agcaccgaag ttaatcagtg ctgtcgcggt gactaaatca aagaacagcg ccgacagcgc
    11281 gacaatcccg accataatga cgttgagtgc cggagtccgc cattttgggt gcacatagcc
    11341 aaacacgcgc tccggaaaca cattgtcacg ccccatcaca tacagcagac gcgacacgct
    11401 ggcatgtgaa gccaggccag acgctaatgt gttcacaaac gtggtgcaga ggaaaatcga
    11461 ctgaaagagc ttgccgccga cgtacagcgc aatttcaggc agtgcggcgt ccgggtcttt
    11521 aaagcggctg atatcgggaa agaacagctg cataaagaac gacgccgcga taaagataac
    11581 gccgccgtat accgccgtca ggaagatggc tttcgggatc acgcgtgcgg cgtccggcgt
    11641 ctcttccgaa agcgtggtca ccgcatcgaa accgaggaac gagaaacaga caatcgtcgc
    11701 accggtaata atcgggatca ggtgcgcgtt ctcgctgata aacggctgaa gtgaccagac
    11761 ggtgccaacg ccttctcctt tatgcagtcc ctgaactacc aggaagataa acaccaccat
    11821 gatggagatt tgcaccagta caaacagggt attgaagtta gcgaccaggt tgacgctctt
    11881 cagattcgcg gcggttaaaa tggcgacgaa ggttaccacc cacacccacg gcggcacttc
    11941 cgggaagagg gcggagagat agattttcgc caacaaaacg ttgatcattg gcaaaaagag
    12001 ataatccagc agtgatgacc agccgaccat aaatccgacg tgcggattaa tcgacttttg
    12061 cgcgtaggta taggccgaac cggcctccgg aaactggcga accagtttgc cgtagctgat
    12121 agcggtaaac agcacgcccg ccagcgccag caaataggac gccggaacgt gaccgtcgct
    12181 aatgccggag acaataccaa aagtatcaaa tacggtcatc ggcgtgagat aggccagacc
    12241 catcattacc acctgccaca atttcagtga ttttcgcaga cgggttttgc cgggttgcgc
    12301 agcaatattc agtggtgaat taatagccat aattgctgcc tcccctgcgt aataaggttt
    12361 gcggtcgtgt caacctgaaa tcaaacctgt ttagcaaggg aagcaaaggg ggcggagtcc
    12421 agtcagtagc aggaggggcg catacgcagc ctccctcgcc atgcggggat ttaatcatcg
    12481 gcatcatctc atttcctcgt cacagtctta atcgttaacg gaagcccgtt gccgcctgcg
    12541 ctccataacg cgacatccgg gaaatgtctg gattaccagc aaatgtcagg cctggctccg
    12601 ctcaggccga tgaaacaacc ccgcatgggg aattacgcgt ttttcaacat ccactcaatt
    12661 tctgtttctg tgatgaggcg ctcaaactgc aacagctcat catttttaca ggcgtgatac
    12721 acatggcaaa accgttcgcc taaatagcgg cgcaggtgat cattctcgat aaactcaccc
    12781 agggcatcgc tctggcgaat cgggaagggt aagccttcct gttccagccc gttgccttcg
    12841 acttcttcct gcaacggcag ctcgttatca aggccatgca aaataccggc aaaaatcgct
    12901 gccatcacca gatatgggtt ggcatcggca cccgccacgc gatattccac gcggtgatta
    12961 tgacggtcgc cgcacggaat acgcagggcg acggtgcggt tgttatggcc ccacgacgcc
    13021 tgcgtcggca catacattcc cggctggaag cggcgatacg agttcacgtt tggtgccagc
    13081 aacgccatcg acgacggcat caggtcaatc atcccggcga gcatcttttt cagcagcggc
    13141 gaatcttcgc cttctgtgtc agaaagcaca ttctcgccac gattattttg catactgata
    13201 tggatatgca tcccgctgcc cgcgtgctct tcatacggct tcgccataaa agtggcgtgc
    13261 atcttatgct tttctgccat cagacgcacc aggcgtttta gcgccagtgc atcatcgcag
    13321 gcttccagca cgttatcggt atggtaaagg ttgatttcaa actgacccgg cgaggcttcg
    13381 gcgaccgcac cgtcggcggg aattaactgt aactgcgcca gttcatcaat atcattgagc
    13441 acgtcggcga agtggttgag gttatcaacg gagtaaacct ggctttgtgt attgcggtca
    13501 tcggtgccgg gcgcgcaggg cggttgcaga tacccttcag cgtcgcgctg gcgatccagt
    13561 aaatagaact ccagctctac cgctacgacc gggaacaatc cgcgctggcg cagctgctgc
    13621 cacaggcggt taagaacgtt ccgcggctca acgtcaaagg gagcgccatc ttcatcgacc
    13681 atggtcagga gcatctgacc gataaactct ggatcggcgg cagaaggggt taaggaaccg
    13741 agaacaggaa cacaagtacg atccggctcg cccatttcct gacccagacc cgcttcttct
    13801 actacgttgc ccagaatatc cattgcaaac accgaggccg ggaaataaca ccctttctcc
    13861 aacttcttca ggcttgaaac agggatacgt tttccacgga agcaaccatt caaatcagta
    13921 agtaaaacat caacatattg cgtattcggg tagcgctcca ggtagcgttt cacttcctgc
    13981 gtaaaggcgc taccccgcct ctcttctgac tgctgaacaa agttctctac ttcaacgata
    14041 ttggtttcca tgattcttcg cctttggttt gttttccgct cgttatcaaa gcgtaaaata
    14101 taatgaccac cattcgaatc tgtatgcaaa ctaaatgttt gtcaaatgtt aaattgagtt
    14161 tgcaaaaatg aaaacccact gctagattga aaaaatattg aacataaagg tcatttaaag
    14221 cgcagtaatg gcgataattt agtccacttt gtgagattga gcatggaaaa tataatgaac
    14281 aatccggtta tcggtgtcgt aatgtgcagg aacaggctta agggtcatgc gacccagact
    14341 ctgcaagaaa agtacctgaa tgccatcatc cacgcaggcg gcttgccaat tgcgctacca
    14401 catgcgctgg cggaaccgtc gttacttgaa caacttttgc cgaaactcga tggcatttat
    14461 cttcctggta gtcccagcaa tgtgcagccg cacctatatg gtgaaaacgg cgatgagcct
    14521 gacgccgatc ccgggcgtga tcttctgagc atggcgttaa ttaatgccgc actcgaaagg
    14581 cgcatcccca ttttcgccat ctgccggggt ttacaggaac tggtggtggc aaccggtggt
    14641 tcgttgcatc gcaagctgtg cgaacagcct gaattgctgg aacatcggga agatcccgaa
    14701 ctgccggtgg aacagcaata tgcaccgtcg catgaagttc aggttgaaga ggggggatta
    14761 ctgtctgcgt tgttacctga atgtagcaac ttttgggtaa actctctgca tggacaaggg
    14821 gcgaaggtcg ttagcccacg gttgcgtgtt gaagctcgct cgccggatgg cttagttgag
    14881 gcggttagcg tcatcaatca tccttttgcg ctgggcgtac agtggcaccc ggaatggaac
    14941 agtagcgagt acgcgctttc gcgtatattg ttcgagggct ttatcaccgc ttgtcagcac
    15001 catatcgctg aaaaacagcg actctgacta ctacagttta aggaaatgca aatatgagtg
    15061 atgagggact ggcgccagga aaacgcttgt cggaaatccg ccagcagcag gggctttcac
    15121 aacgtcgtgc cgccgaactc tccgggctga ctcacagtgc tatcagtacg atagaacaag
    15181 ataaagtcag ccctgccatc agtacgctgc aaaagctgct gaaggtgtat ggtctgtcac
    15241 tctcggaatt cttttccgag ccggaaaaac ctgatgagcc gcaggtcgtc attaatcagg
    15301 acgacttaat tgagatgggc agtcagggtg tgtcaatgaa gctggttcat aacggtaacc
    15361 cgaatcgcac gctggcgatg atctttgaaa cgtaccagcc gggcacaacc actggggaaa
    15421 gaattaagca tcagggtgag gaaataggca ctgtactgga aggtgaaatt gttctgacga
    15481 ttaatggtca ggattatcat ctcgtcgcgg ggcaaagtta tgccattaat accggcatcc
    15541 cgcacagttt cagtaatacg tcggcaggta tttgccgaat tatcagcgcc catacgccca
    15601 ccacgtttta atcttcttgt tctgtaagcc gggtaagcgc aatgcgcccg gcaatctata
    15661 cacaaaatca ttcaagttgc atcaaggcgg caagtgagcg aatcccgatg agcttactca
    15721 ggtaagtgat tcgggggagc aaacgcagcc aaggcagagg cggcttgaag gatgaagtgt
    15781 atataaagaa tgtcgccaga aataaacggg catgcggccc ggggatctct gcgccctgac
    15841 gttcacaaac tgcatatatc tgatagacgt gaaacaggag tcataatgaa ttttcatcat
    15901 ctggcttact ggcaggataa agcgttaagt ctcgccattg aaaaccgctt atttattaac
    15961 ggtgaatata ctgctgcggc ggaaaatgaa acctttgaaa ccgttgatcc ggtcacccag
    16021 gcaccgctgg cgaaaattgc ccgcggcaag agcgtcgata tcgaccgtgc ggtgagcgca
    16081 gcacgcggcg tatttgaacg cggcgactgg tcactctctt ctccggctaa acgtaaagcg
    16141 gtactgaata aactcgccga tttaatggaa gcccacgccg aagagctggc actgctggaa
    16201 actctcgaca ccggcaaacc gattcgtcac agtctgcgtg atgatattcc cggcgcggcg
    16261 cgcgccattc gctggtacgc cgaagcgatc gacaaagtgt atggcgaagt ggcgaccacc
    16321 agtagccatg agctggcgat gatcgtgcgt gaaccggtcg gcgtgattgc cgccatcgta
    16381 ccgtggaact ttccgctgct gctgacctgc tggaagcttg gcccggcgct ggcagcaggg
    16441 aacagcgttg tcctgaaacc gtctgaaaaa tcaccgctca gtgcgattcg tctcgcgggg
    16501 ctggcgaaag aagcaggctt gccggatggt gttttgaacg tggtgacagg ttttggtcat
    16561 gaagcggggc aggcgctgtc gcgtcataac gatatcgacg ccattgcctt taccggttcg
    16621 acccgtaccg ggaaacagct gctgaaagat gcgggcgaga gcaacatgaa acgcgtctgg
    16681 ctggaagcgg gcggcaaaag cgccaacatc gttttcgccg attgtccgga tctgcaaaaa
    16741 gcggcaagcg ccaccgccgc aggcatcttc tacaaccagg gccaggtgtg catcgccgga
    16801 acgcgtttgt tactggaaga gagcatcgcc gatgaattct tagccctgtt aaaacagcag
    16861 gcgcaaaact ggcagccggg ccatccactt gatcccgcaa ccaccatggg caccttaatc
    16921 gacagcgctc acgctgactc ggtccatagc tttattcggg aaggcgaaag caaagggcaa
    16981 ctgctgctgg atggtcgtaa cgctgagctg gctgccgcca tcggcccgac catctttgtg
    17041 gatgtagacc cgaatgcgtc cttaagccgc gaagagattt tcggtccggt gctggtggtc
    17101 acgcgtttca catcagaaga ccaggcgcta cagcttgcca acgacagcca gtacggcctt
    17161 ggcgcggcgg tatggacgcg cgacctctcc cgcgcgcacc gcatgagccg ccgcctgaag
    17221 gctggttccg tcttcgtcaa taactacaac gacggcgata tgaccgtgcc gtttggcggc
    17281 tataagcaga gcggcaacgg tcgcgacaaa tccctgcatg cccttgaaaa attcaccgaa
    17341 ctgaaaacca tctggataag cctggaggcc tgaaatgacc gaacatacca gcagttacta
    17401 cgccgccagt gcgaataaat acgcgccatt cgacacgctg aatgagtcga tttcctgcga
    17461 cgtttgcgtg gtcggcggcg gctataccgg gctctcttcc gcgctgcatc tggcggaagc
    17521 gggctttgac gtagtggttc tcgaagcctc acgcatcggc tttggcgcaa gcgggcgcaa
    17581 tggcgggcag cttgtgaact cctacagccg cgacatcgac gtgatcgaaa aaagctacgg
    17641 catggacacc gcccgtatgc tcggcagcat gatgttcgaa ggtggtgaga tcatccgcga
    17701 acgtatcaaa cgttatcaga ttgactgcga ctaccgcccc ggcggcctgt ttgtggcgat
    17761 gaatgataaa cagctcgcca cacttgaaga gcagaaagag aactgggaac gctacggcaa
    17821 taaacagctg gaattgctgg acgccaacgc cattcgccgt gaagtagcca gcgatcgcta
    17881 taccggtgcg ctgctggatc acagcggtgg gcatattcat ccgctaaacc tcgccattgg
    17941 cgaagcggac gccatccgcc tcaacggcgg gcgcgtgtat gaactttctg ccgtgacgca
    18001 gatccagcac accacgccag cggtggtgcg cactgccaaa ggtcaggtga cggcgaagta
    18061 tgtgattgtc gccgggaatg cgtatctggg cgataaagta gagccggaac tggcgaaacg
    18121 cagcatgccg tgcggcacgc aggtgatcac caccgaacgg ctgtcggaag atttagcccg
    18181 ttcgctgatc ccgaaaaact actgtgtgga agactgtaat tatctgcttg attactaccg
    18241 tcttaccgcc gacaaccgtt tgctgtacgg cggcggcgtg gtctacggcg cgcgcgaccc
    18301 ggatgacgtt gagcgtcttg tggtgccgaa actgctgaaa accttcccgc aactgaaggg
    18361 cgtcaaaatc gactaccgct ggacgggcaa cttcctgctg accctttcac ggatgccgca
    18421 gttcggtcgc ctcgatacca acatctatta catgcagggc tacagcggcc acggcgtcac
    18481 ctgtacccat cttgccgggc gtctgattgc cgaactgctg cgcggcgacg ccgaacgttt
    18541 cgatgccttc gccaatctgc cgcattaccc gttccccggc gggcgcacgc tgcgagtacc
    18601 gtttaccgcg atgggcgcag cctattacag cctgcgcgat cgtctgggcg tttaatttcc
    18661 gattaacagt gaagagtcaa aaggtgtgaa acatgagcaa caatgaattc catcagcgtc
    18721 gtctttctgc taccccacgc ggggttggcg tgatgtgtaa cttcttcgcc cagtcggctg
    18781 aaaacgccac gctgaaggat gttgagggca acgagtacat cgatttcgcc gcaggcattg
    18841 cggtgctaaa taccggccac cgccaccctg aactggtcga ggcggtagag cagcaactgc
    18901 aacagtttac ccacaccgcg tatcagattg tgccgtatga aagctacgtc accctggcgg
    18961 agaaaatcaa cgcccttgcc ccggtgagcg ggcaggccaa aaccgcgttc ttcactaccg
    19021 gcgcggaagc ggtggaaaac gcggtgaaaa ttgctcgcgc ccataccgga cgccctggcg
    19081 tgattgcgtt tagcggcggc ttccacggtc gtacgtatat gactatggcg ttaactggaa
    19141 aggtcgctcc gtacaaaatc ggcttcggcc cgttccctgg ttcggtgtat cacgtacctt
    19201 atccgtcaga tttacacggc atttcaacac aggactccct cgacgccatc gaacgcttgt
    19261 ttaaatcaga catcgaagcg aagcaggtgg cggcgattat tttcgaaccg gtgcagggcg
    19321 agggcggttt caacgttgcg ccaaaagagc tggttgccgc cattcgccgc ctgtgcgacg
    19381 agcacggcat tgtgatgatt gccgatgaag tgcaaagcgg ctttgcgcgt accggtaagc
    19441 tgtttgccat ggatcattac gtcgataagc cggatttaat gacgatggcg aaaagcctcg
    19501 cgggcgggat gccgctttcg ggcgtggtcg gtaacgcgaa tattatggac gcgcccgcgc
    19561 cgggcgggtt gggtggcacc tacgccggga acccgctggc ggtggctgcc gcgcacgcgg
    19621 tgctcaatat tatcgacaaa gaatcactct gcgaacgcgc gaatcaactg ggccagcgtc
    19681 tcaaaaacac gttgattgat gccaaagaaa gcgttccggc cattgctgcg gtacgcggcc
    19741 tggggtcgat gattgcggca gagtttaacg atccgcaaac gggcgagccg tcagcggcga
    19801 ttgcacagaa aatccagcaa cgcgcgctgg cgcaggggct gctcctgctg acctgtggcg
    19861 catacggcaa cgtgattcgc ttcctgtatc cgctgaccat cccggatgcg caattcgatg
    19921 cggcaatgaa aattttgcag gatgcgctga gcgattaata gcccaacgca ataatgtctg
    19981 atgcgctgcg cttatcaggc ctgcaaacga cgtattgatt atgtatgccg aataaagcat
    20041 tcacgccgca tccggcaagt tgtattgctc aacttcgcta aatctggtgc tttttcaaca
    20101 acgcgcggaa ctgatgataa gttaacccca gtaattcagc cgcgcgcttc tggttatatt
    20161 tcccttgttg caaactgagt tgcagcaact ctttttcctg ctgcatctga aactcacgta
    20221 aatccagcgg cagtgttgga agcgaggtgg tttctgaaac ggcgatagcg tcttcaggcg
    20281 gacgccgttt aaagggatca ataatgatgt catcaagcgg ataatcgctg gtgccgtggc
    20341 gatacactga acgttccacc acgtttttca attcacgaat atttcccggc caacgataat
    20401 tcagcaatgt ttctctggcg cgctccgtaa accccgggaa cagaggcagc ttgatttccc
    20461 gacacatctg gatggcaaag tgttctgcca tcaacattat gtcgctttcg cgctcgcgca
    20521 gtggtggcag ttgtacaaca tcaaaagcca gtcggtcgag caggtcagcg cgaaaagtgc
    20581 cttcattgac catcgccggg agatcggcat tcgtcgcgca taccaaccgc acattcacct
    20641 gcaatggctg gctgccgcca acgcgctcca gttcaccgta ctcaatcacg cgcaataatt
    20701 tctcctgcac catcatcggt gccgtagcga gttcatcaag aaatagcgta ccgccgtcgg
    20761 cacgttcaaa tctccctgga tgacgttttt gcgcaccggt aaacgccccc gcttcgtgac
    20821 caaacagttc ggaatccagc agattttcat ttaacgccgc gcagttaagg gaaataaacg
    20881 gcccttgcca acgggaggag agataatgca ggcggctggc aatcagctct ttaccggtgc
    20941 cgcgttcgcc gatgatgagc accggtttgt ccagcggtgc gagatgcgaa acctgttcca
    21001 gcacttcgag aaagctgttc gcctcaccaa gtaaattatc tttgtattct gccatgatga
    21061 aattcgccac ttgttagtgt aattcgctaa ctcatcctgg catgttgctg ttgattcttc
    21121 aatcagatct ttataaatca aaaagataaa aaattggcac gcaaattgta ttaacagttc
    21181 agcaggacaa tcctgaacgc agaaatcaag aggacaacat tatgggtatt ttttctcgct
    21241 ttgccgacat cgtgaatgcc aacatcaacg ctctgttaga gaaagcggaa gatccacaga
    21301 aactggttcg tctgatgatc caggagatgg aagatacact ggttgaagta cgttctactt
    21361 cggcgcgcgc gttggcagaa aagaaacagc tgactcgccg tattgaacaa gcgtcggcgc
    21421 gtgaagttga atggcaggaa aaagccgaac tggcgctgct gaaagagaga gaggatctgg
    21481 cacgtgcagc gttaattgaa aaacagaaac tgaccgattt gattaagagc ctggaacatg
    21541 aagtgacgct ggtggacgac acgctggcac gcatgaagaa agagatcggt gagctggaaa
    21601 acaaattgag cgaaacacgc gctcgccagc aggcattgat gttacgtcat caggcggcaa
    21661 actcgtcgcg cgatgtgcgt cgtcagctgg acagtggcaa actggatgaa gcaatggctc
    21721 gtttcgaatc tttcgaacgt cgtattgacc agatggaagc ggaagcagaa agccacagct
    21781 tcggtaaaca aaaatcgctg gacgatcagt ttgccgaact gaaagccgat gatgcaatca
    21841 gcgaacaact ggcacaatta aaagccaaaa tgaagcaaga caatcaataa taacatccag
    21901 gcggcgtccg aacgcgccgc cgctcatcgt ctaaggagta cttatgagcg cgctatttct
    21961 ggctattccg ttaaccattt ttgtgctgtt tgttttaccg atctggttat ggctgcatta
    22021 cagcaatcgt tctggtcgca gtgaattgtc gcaaagtgag cagcagcgat tagcgcaact
    22081 ggctgatgaa gcaaaacgga tgcgcgaacg tattcaggcg ctggaatcta ttcttgatgc
    22141 agaacatccg aactggaggg atcgctaatg gcgggcatta atctcaataa aaaattatgg
    22201 cgtattccac agcagggcat ggtccgcggc gtctgcgccg ggattgccaa ctattttgat
    22261 gtaccggtaa aactggtgcg tatcctggtg gtgctgtcga ttttcttcgg tctggcgctg
    22321 tttaccctgg ttgcttacat cattttgtca tttgcgcttg atccaatgcc ggacaacatg
    22381 gcctttggtg agcagctacc ttccagcagc gaattgctgg atgaagtcga ccgtgaactg
    22441 gcggcaagtg aaacgcgttt acgcgagatg gaacgttatg tcacttccga tactttcacg
    22501 ttacgtagcc gtttccgtca actgtgagga aagttatgaa tactcgctgg caacaggccg
    22561 ggcaaaaggt aaagcctggt ttcaaattag caggcaagct ggtacttctt actgcactgc
    22621 gctatggccc ggcgggtgtg gcgggctggg cgataaaatc agttgctcgc cgaccgctga
    22681 aaatgttgct ggctgtggcg ctggaaccgc tgttaagtcg ggctgctaat aaactggcac
    22741 agcgttataa aaggtgaggg gagtttcgca aaaattgtta aatctcaggc gtataatgga
    22801 tggcaatttt catccataga aggacgctta catgtttaaa aaaggcttac ttgctctggc
    22861 actggtgttt tcactgcccg ttttcgccgc tgaacactgg atcgatgttc gtgttccaga
    22921 gcagtatcag caagagcacg ttcagggggc catcaatatt cccctgaagg aagtgaaaga
    22981 gcgcattgcc accgccgttc cggataaaaa cgacaccgtg aaagtgtatt gcaatgccgg
    23041 acgccagtca gggcaagcaa aagagatcct tagcgagatg ggatataccc acgttgagaa
    23101 cgccggtggc ctgaaagaca tcgcaatgcc gaaggtcaaa ggttaaaaga attcaccatg
    23161 agcggcgctt atgcgccgtt ttttttctgt cacaccttat ttacatcccc atagattatt
    23221 tgcgtcagct cacaaatacg ctttttccct ggtaaaaaat gatttcctgc gtgactaaaa
    23281 cccttgtgct caattgacag tttattttct gcggagtagt ctctcgtttc atgggaccgc
    23341 taccacggaa aggcaacatg aaacagaaaa ttacggatta cctggacgaa atctacggtg
    23401 gaacatttac cgcaactcat ttacagaaac ttgtaacgcg tcttgagagt gcgaaacgat
    23461 taattacaca gcgacgtaaa aaacactggg atgaaagtga tgtcgtgtta attacctatg
    23521 ccgatcaatt tcacagcaat gatttaaaac cattacccac atttaatcag ttttaccatc
    23581 aatggctgca aagcattttt tcacatgttc atttgttgcc gttttatcca tggtcatctg
    23641 atgatggctt ttcggtaatt gattatcatc aggtcgccag tgaagcgggg gagtggcagg
    23701 atattcagca actcggtgaa tgcagtcatt taatgtttga ttttgtctgc aaccatatgt
    23761 cggcaaaaag tgaatggttt aaaaactatt tacaacagca tccaggtttt gaagattttt
    23821 ttattgccgt tgacccgcaa accgatctca gcgccgtcac tcgcccgcgt gcgttaccgt
    23881 tattaacgcc attccagatg cgcgatcatt caacgcgcca tttatggacc acctttagtg
    23941 acgatcaaat tgacctgaat taccgtagcc ctgaagtgtt gctggcgatg gtggatgttt
    24001 tactctgtta ccttgcgaaa ggtgctgagt atgtccgcct ggatgccgtt ggctttatgt
    24061 ggaaagagcc gggaacaagc tgcatccatc tggaaaaaac acatctgatt atcaaactgt
    24121 tacggtcgat tattgataac gttgcgccag gtacagtgat cattaccgag accaatgttc
    24181 cgcataaaga caacattgct tactttggcg caggcgatga cgaagcacat atggtgtacc
    24241 agttctcgct gccgccactg gtgctgcatg cggtgcaaaa acagaacgtt gaggcgcttt
    24301 gtgcgtgggc gcaaaacctg acactacctt ccagcaacac cacctggttt aacttcctcg
    24361 cctctcacga tggcatcggg ctaaacccgc tacggggctt gttgcctgaa agcgaaatat
    24421 tagagctggt cgaggcgtta cagcaggaag gtgcattagt aaactggaaa aataatcccg
    24481 acggtacacg cagtccgtat gaaataaatg tgacctatat ggatgcgtta agccgccgtg
    24541 agagtagcga tgaagaacgt tgcgccaggt ttattctcgc ccatgcgatt ttgttaagtt
    24601 tccccggtgt gccagcgata tatattcaaa gtattcttgg ctcgcgtaat gattacgcag
    24661 gtgtcgaaaa actcggatat aaccgtgcga ttaaccgtaa aaaatatcac agtaaagaga
    24721 taacccgaga actgaacgat gaagctacat taaggcatgc ggtatatcat gagttgtcgc
    24781 gtttaattac acttcgtcgc agccataacg agtttcatcc ggataataat tttaccatcg
    24841 atacgattaa ttcatccgta atgcgtattc aaagaagtaa cgctgatggt aattgtctga
    24901 ctggattgtt taatgtcagt aaaaatattc agcatgtaaa tattactaat ctgcatggtc
    24961 gggatctgat tagtgaagtt gatatattgg gtaatgaaat aacgctgcgc ccctggcagg
    25021 ttatgtggat taaataaaaa ggaacatctc atgattaaat caaaaatcgt gctgttatca
    25081 gcactggttt cctgcgccct gatttcaggc tgtaaagaag aaaataaaac gaatgtatcc
    25141 atcgaattta tgcattcttc ggtggagcag gagcgccagg ccgttatcag taaattgatt
    25201 gcccgttttg aaaaagaaaa ccctggcatc accgttaagc aagtgcccgt ggaagaagat
    25261 gcctataaca ctaaagtcat tactctttca cgtagcggtt cgctgccgga agtgatagaa
    25321 accagccatg actacgccaa agtgatggac aaagagcagc ttatcgatcg caaagcggtt
    25381 gccacagtca tcagcaacgt tggtgaaggc gcgttttacg atggcgtact gcgtattgtg
    25441 cgtaccgaag atggtagcgc atggaccggt gttcctgtca gcgcctggat tggcggtatc
    25501 tggtatcgca aagatgtgct ggcaaaagcg gggcttgagg agccgaaaaa ctggcaacag
    25561 ctgctggacg ttgcacagaa actgaatgac ccggcgaata aaaaatacgg cattgcgctg
    25621 cctacagcag aaagcgtgtt gacggaacaa tccttctccc agtttgcgtt atccaaccag
    25681 gctaacgtct ttaacgccga aggcaaaatc acccttgata caccagagat gatgcaggca
    25741 ctgacctatt accgcgacct tgctgccaac actatgccgg gttctaacga catcatggaa
    25801 gtgaaagacg cctttatgaa cggcaccgcg ccgatggcga tttactccac ctatatcctt
    25861 ccggctgtga ttaaagaagg cgacccgaaa aacgtcggtt tcgtggtgcc aaccgagaaa
    25921 aactctgcgg tctacggcat gttgacctcg ctgaccatta ccgccgggca aaagaccgaa
    25981 gagacggaag cagcagaaaa atttgtcacc tttatggagc aggcagacaa cattgccgac
    26041 tgggtgatga tgtcgccagg tgctgcgctg ccggtgaata aagcggttgt cactaccgcc
    26101 acctggaaag acaacgacgt tattaaggcg ctgggtgaac taccgaatca gctaatcggt
    26161 gaactgccaa atattcaggt ctttggtgca gtaggggata aaaactttac ccgcatgggt
    26221 gatgtgacgg ggtctggcgt ggtgagctcc atggtgcata acgtcaccgt gggtaaagcc
    26281 gatctccctg gcacgctgca agcgagccag aaaaagctgg atgaactgat cgaacagcac
    26341 taaacccagg acaggaatcc gcaatgaaca ggcttttttc aggtcgttcc gatatgccct
    26401 ttgcgctgct gcttctcgcg cccagcttat tactgctggg tggtctggtg gcctggccaa
    26461 tggtgtcgaa tatcgaaatc agttttttac gtctgccgct caatcccaac atcgagtcaa
    26521 cctttgttgg ggtgagtaac tatgtgcgta tcctctccga tcccggcttc tggcactcgc
    26581 tgtggatgac ggtctggtat accgcgctgg tggtggcggg cagcaccgtg ctcgggctgg
    26641 cagtggcgat gtttttcaac cgcgagttcc gtctgcgcaa aaccgcgcgt tcgctggtga
    26701 tcctctccta cgtaacgccg tccatttcgc tggtgtttgc ctggaaatac atgttcaaca
    26761 acggctacgg cattgtgaac tacctcggcg tcgatcttct gcatctctat gagcaggcac
    26821 cactgtggtt cgataatccg ggcagtagct ttgtgctggt ggtgctgttc gccatctggc
    26881 gctacttccc gtatgccttt atctcgtttc tggcgatttt gcagaccatc gacaaatcgc
    26941 tgtacgaagc ggcagagatg gatggcgcta atgcctggca gcggtttcgc atcgtcacgc
    27001 tgcccgcgat tatgccagtc ctggcgacgg tggtgaccct gcgcaccatc tggatgttct
    27061 acatgttcgc ggatgtttat ttgctgacga ccaaggtcga tattctcggt gtatatctct
    27121 acaaaaccgc ctttgccttt aatgatttag gaaaagcggc ggcgatctcg gtggtgctct
    27181 tcatcattat tttcgctgtc attctgctga ccaggaaacg ggtgaacctc aatggcaaca
    27241 aataaacgca cactcagtcg catcggtttt tactgcgggc tggcgctgtt tctcatcatc
    27301 acgctgtttc cattttttgt gatgctgatg acctcgttca agggcgcgaa agaggcgatc
    27361 tcactgcatc ctacgctgct gccgcagcag tggacgctgg agcattacgt cgacattttt
    27421 aacccgatga ttttcccgtt tgtcgactac ttccgtaaca gtctggtggt gtctgtggtt
    27481 tcatccgtgg tggcggtatt cctcggcatt cttggggctt acgcgctttc ccgcctgcgc
    27541 tttaaaggtc ggatgaccat caacgccagc ttttacacgg tgtatatgtt ctctggcatt
    27601 ttgctggtgg tgccgctgtt caaaatcatc accgcgctgg gcatttacga cactgagatg
    27661 gcgctgatca tcaccatggt gacgcaaact ctgcccaccg ccgtgtttat gctgaaaagc
    27721 tacttcgaca ccatccccga tgagatcgaa gaagcggcga tgatggatgg cctcaaccgt
    27781 ctgcaaatta tcttccgcat caccgtgccg ttggcgatgt ccgggctgat atccgtgttc
    27841 gtctactgct ttatggtggc gtggaacgac tatctgtttg cgtcgatctt cctctccagc
    27901 gccagcaatt tcaccttacc ggtgggcctg aacgcgctgt tcagtacgcc agattacatc
    27961 tggggacgga tgatggcggc ctcactggtg accgcattgc cggtggtgat tatgtatgcg
    28021 ctttcggaac gttttattaa gagtggtttg accgccggtg gcgtgaaggg ctaaagcggc
    28081 cagtttcttt acaaggagtt ttaaatgaaa aagttagtag ccacagcacc gcgtgttgct
    28141 gcgctggttg agtatgaaga tcgggcgatt ttagccaatg aagtgaagat ccgcgtgcgt
    28201 ttcggcgcac cgaaacacgg aacggaagtg gtcgacttcc gcgccgccag cccgtttatc
    28261 gatgaagact ttaacggcga atggcagatg ttcactccgc gtccggcaga tgcgccgcgc
    28321 ggcattgagt ttggcaaatt ccagcttgga aacatggtgg ttggcgacat tatcgagtgc
    28381 ggcagcgacg ttaccgacta cgcggtggac gacagcgtat gcggctacgg cccgctctcc
    28441 gagacggtca tcattaacgc ggtgaataac tacaagctgc gcaaaatgcc ggaaggcagc
    28501 tcgtggaaaa acgccgtttg ttacgacccg gcgcagtttg ccatgagcgg cgtgcgcgat
    28561 gccaacgtgc gcgtgggtga ttttgttgtg gtggtagggc ttggcgcgat tggtcaaatt
    28621 gccattcaac tggcgaaacg cgctggcgcg tcggtggtga ttggcgtcga tcctatcgcc
    28681 catcgctgtg atattgcccg tcgccacggc gcggatttct gccttaaccc cattggtact
    28741 gacgtaggca aagagatcaa aacgctgacc ggcaagcagg gtgccgatgt gattatcgaa
    28801 accagcggct atgccgacgc gctgcaatcg gcgttccgcg gtctggccta cggtggcacc
    28861 atctcctatg tcgcgtttgc caaaccattt gctgaaggtt ttaacctcgg acgcgaagcg
    28921 catttcaata acgccaaaat tgttttctcc cgcgcgtgca gcgaaccgaa cccggattat
    28981 ccgcgctgga gccgtaagcg tattgaagaa acctgctggg aactgctgat gaacggttat
    29041 ctcaattgcg aagatttaat cgacccggta gtgacctttg ccaatagccc ggaaagctac
    29101 atgcagtatg tcgatcagca tccggaacag agcatcaaaa tgggcgttac gttttaattc
    29161 aaaggaatga aaaaatgaaa ataggcacac agaatcaggc attctttccg gaaaacattc
    29221 tggagaaatt tcgttatatc aaagagatgg gcttcgatgg ttttgagatt gacggcaaac
    29281 tgctggtcaa caacatcgaa gaagtcaaag cagcaatcaa agaaaccggt ttaccggtga
    29341 ccaccgcctg cggtggctat gacggctgga ttggtgactt tattgaagag cgtcgtctta
    29401 atggcttaaa gcagatcgaa cgcattctcg aagcgctggc agaagtgggg ggtaagggta
    29461 tcgtcgttcc ggcagcgtgg ggcatgttta ccttccgctt accgccgatg acctcgccgc
    29521 gcagcctgaa cggcgatcgt aaaatggtga gtgattccct gcgcgtactt gaacaggtcg
    29581 ccgcgcgtac cggaaccgtg gtgtatctcg aaccgttaaa ccgctatcag gatcatatga
    29641 tcaacaccct cgccgatgcc cgccgttaca tcgtcgaaaa cgatcttaaa catgtacaga
    29701 ttatcggcga tttctatcac atgaatatcg aagaagataa cctggcgcag gcgctgcatg
    29761 acaaccgcga cctgctcggt catgtgcata ttgcggataa ccatcgctac cagccgggca
    29821 gcggcaccct ggatttccac gcgctgtttg aacagctgcg cgcggataac tatcagggct
    29881 atgtagtgta cgaagggcgt atccgggcgg aagatcctgc ccaggcgtac cgtgattcgt
    29941 tggcctggtt gcgtacctgc taagaggtct ttgtgaaaag tgcaatgaca agctctccgc
    30001 tgcgggtcgc gataataggc gcaggccagg tggcggataa ggttcatgct tcgtactact
    30061 gcacccgcaa cgatctggaa ctggtggctg tctgtgacag ccgcctttcc caggcgcagg
    30121 cgctggcaga aaaatacggg aatgcatccg tgtgggacga tccgcaggcc atgctgctgg
    30181 cggtgaaacc tgatgtggtt agcgtctgct cacctaaccg ttttcattac gaacataccc
    30241 tgatggcact ggaagcgggc tgccatgtga tgtgcgaaaa accgcccgcc atgacgccag
    30301 aacaggcgcg ggaaatgtgc gataccgcgc gcaaactggg caaggtgctg gcctacgact
    30361 ttcaccatcg ttttgcgctc gatacgcaac agctgcgtga acaggtgacc aacggcgttt
    30421 tgggagagat ttacgttacc accgcccgcg ccctgcgtcg ctgcggcgtt cccggctggg
    30481 gtgtctttac caataaagaa ctgcagggtg gtggcccgct gatcgacatc ggcattcata
    30541 tgctggatgc tgcgatgtat gtgctgggct ttccggcggt gaaaagcgtg aatgcgcata
    30601 gctttcaaaa gatcggcacg caaaagagct gtggtcaatt tggcgagtgg gatccggcaa
    30661 cttacagcgt cgaagattcg ctgtttggca ccattgaatt tcataacggc ggcattctgt
    30721 ggctggaaac gtcatttgca ctcaacatcc gccaacagtc gattatgaac gtcagctttt
    30781 gtggtgataa agccggtgcg acgctgtttc cagcacatat ctacaccgat aacaacggtg
    30841 aattaatgac gctgatgcaa cgggaaatgg cagacgacaa ccgccatttg cgcagcatgg
    30901 aagcctttat caatcacgta cagggcaagc ccgtgatgat agccgacgcc gagcaggggt
    30961 acatcatcca gcaactggtg gcggcgttgt atcaatccgc agaaacaggg acgcgtgtgg
    31021 aattatgacc aggccagtaa cgttatcaga accccatttc agccagcata ccctgaacaa
    31081 gtatgcatcg ctgatggcgc aggggaacgg ctatcttggg cttcgcgcca gccatgaaga
    31141 agattacacg cgccagacgc gagggatgta tctggcgggg ttgtatcatc gggcgggtaa
    31201 aggtgaaatc aacgaactgg tgaacctgcc tgatatcttg gggatggaga ttgccataaa
    31261 tggtgaggtt ttctcgttat cccacgaaac ctggcagcgt gagcttgact ttgccagtgg
    31321 cgaattacgc cgcaacgttg tctggcgtac cagcaacggc gcaggttaca ccatcgccag
    31381 ccgtcgcttt gtttcggcag accaactgcc gctcattgcg ctggaaatca ctattacgcc
    31441 actggacgcc gacgcgtcag tgctgatttc aacaggtatc gacgccacgc aaaccaacca
    31501 cggtcgccaa catctcgacg aaacccaggt gcgggtgttt ggtcagcatt tgatgcaggg
    31561 gatctacacc acccaggatg gacgcagtga tgtggccatc agctgttgct gtaaggtgag
    31621 cggtgatgtg cagcaatgct ataccgccaa agagcgccgt ttactgcaac ataccagtgc
    31681 gcagcttcat gcaggcgaga cagtgacgtt gcaaaaactg gtgtggatcg actggcggga
    31741 tgacaggcaa gccgttttag acgagtgggg cagcgcgtcg cttcgccagc ttgaaatgtg
    31801 cgcgcagcag agttacgacc aacttcttgc agtatcaaca gaaaactggc gtcaatggtg
    31861 gcagaaacgt cgtatcgcgg taaatggcgg cgatgcgcac gatcagcaag cgttagatta
    31921 tgcgctttat catctgcgca tcatgacgcc tgcccacgac gagcgcagca gcattgcggc
    31981 aaaaggctta accggcgaag gctacaaagg ccacgttttc tgggatacag aagtattttt
    32041 gttaccgttt catctgttta gcgatccgac ggttgcccga agtttactgc gttatcgctg
    32101 gcacaacttg ccaggcgcgc aggagaaagc gcgacgcaac ggctggcagg gcgcgctatt
    32161 tccgtgggaa agcgcgcgca gcggcgaaga agagacgccg gaatttgccg ccattaacat
    32221 tcgcaccggg ctgcggcaaa aagtggcctc ggcgcaggcg gaacatcatc tggtggccga
    32281 tatcgcctgg gcggttattc aatactggca gaccacgggg gatgaaagtt tcatcgctca
    32341 tgaaggcatg gcgctacttc tggaaactgc aaagttctgg attagccgcg cggtgagggt
    32401 taacgaccgt ctggaaattc atgatgttat tgggccagac gaatataccg aacatgtcaa
    32461 taataacgcc ttcaccagct atatggcgta ttacaacgtc cagcaggcgc tgagtattgc
    32521 ccgccagttt ggctgtagcg acgacgcgtt tattcatcgt gccgaaatgt tccttaaaga
    32581 actgcggctg ccagaaattc agcccgacgg cgttttgccg caggatgatt cgtttatggc
    32641 taagccggcg attaatctgg cgaaatacaa agcggcggcg gggaagcaaa ccattctgct
    32701 ggattattca cgcgcagaag tgaacgagat gcaaatcctc aaacaagctg atgtggtgat
    32761 gctcaattac atgctgccgg agcagttctc agcggcatcg tgtcttgcca atctgcaatt
    32821 ttatgaaccg cgcactattc acgactcgtc attaagtaaa gcaatccacg gcattgttgc
    32881 cgcacgctgt ggcctgctga cccaaagtta tcagttctgg cgcgagggga ctgaaatcga
    32941 tcttggtgct gatccgcata gttgtgatga tggtatccat gctgccgcaa ctggcgctat
    33001 ctggctgggg gcgattcagg gttttgccgg ggtgagcgtg cgtgacggtg aattgcatct
    33061 caatccggcg ttacctgagc agtggcaaca gttgtctttc cctctgttct ggcagggctg
    33121 cgaattacag gtcactcttg acgcgcagcg tattgcgatt cgaacttctg cgcccgtttc
    33181 actgcgtttg aacggtcagc ttatatccgt ggctgaagaa tctgttttct gtttgggtga
    33241 ttttattttg ccctacaatg ggaccgctac caaacatcag gaggatgaat gaaactgcaa
    33301 ggggtaattt tcgatctgga tggtgtaatc accgataccg cgcatctgca tttccaggcg
    33361 tggcagcaga ttgccgctga aattggcatc agcattgatg cgcagtttaa cgaatcccta
    33421 aaagggatca gccgcgatga gtctctgcgg tgcattctgc aacacggggg caaagagggc
    33481 gactttaact cgcaggagag ggcgcaactg gcgtatcgca aaaatctgct ctatgtccac
    33541 tcactgtgcg agttgacggt caacgctgtt ctacccggca ttcgctcttt gctggcagat
    33601 ctccgcgcac aacagatccc ggtagggctg gcttctgtct ccctcaatgc gccgacgatt
    33661 ttagatgcac tggagctgcg tgagtttttc accttctgcg cggatgcttc ccaacttaaa
    33721 aactcgaaac cggatccgga aatctttctc gctgcctgtg ccgggctggg cgtgccgccg
    33781 caggcatgta tcggcattga agatgcgcag gcgggcattg acgccattaa cgccagcggt
    33841 atgcgctcgg tggggatcgg cgcgggctta accggggcgc aattactgtt gccttcaacg
    33901 gaatcactca cctggccgcg gttatcggcc ttctggcaaa acgtatagca aaggaatcaa
    33961 catggctcag ctttcgttac aacatattca aaaaatctac gataaccagg tgcatgtggt
    34021 gaaggacttc aacctggaaa ttgccgataa agagttcatc gtgtttgtcg gcccgtcggg
    34081 ctgcggtaag tcgaccaccc tgcgcatgat tgccgggctt gaggagatca gcggcggcga
    34141 tctgttgatc gacggcaaac gaatgaatga cgttccagcc aaagcacgca atatagcgat
    34201 ggtgttccag aactacgcgt tgtatccgca tatgacggtt tacgacaaca tggcgtttgg
    34261 cctgaagatg caaaaaatcg ccaaagaggt gattgatgag cgggtgaact gggcggcgca
    34321 aattctcggc ctgcgtgagt acctgaaacg caagccgggc gcgctttccg gcgggcaacg
    34381 ccagcgtgtg gcgctcgggc gggcgattgt gcgcgaagcg ggcgtgtttt taatggatga
    34441 accgctctct aaccttgatg ccaagctgcg cgtgcaaatg cgcgcagaga tcagcaagct
    34501 acaccagaag ctgaatacca ccatgatcta cgtgacccac gatcagaccg aagcgatgac
    34561 catggcgacg cggattgtga ttatgaagga tgggattgtt cagcaagttg gtgcgccgaa
    34621 aacggtttat aaccaaccag cgaatatgtt tgttgccgga tttattggat caccggcgat
    34681 gaattttatt cgtggcacga tcgatggcga taaattcgtt acggaaacgc ttaaattaac
    34741 cattcccgaa gagaaattag cggttctgaa aacacaggga agtttgcata agcccatcgt
    34801 gatgggaata cgtccggaag atattcatcc ggacgcgcaa gaggaaaata acatttccgc
    34861 caaaattagc gtggcagaat taaccggtgc ggaatttatg ctctacacca cggttggggg
    34921 gcacgagtta gtggtccgtg ctggtgcgtt aaatgattat catgcaggag aaaatatcac
    34981 tattcatttt gatatgacga aatgtcattt ctttgatgca gaaacggaaa tagcaattcg
    35041 ctaaatacag ggggaaggca ttcccccaga ataatacaag gaacaataat gaaaaagtta
    35101 ttaccctgta ccgcactggt gatgtgtgcg ggaatggcct gcgcacaggc cgaggaaagg
    35161 aacgactggc actttaatat cggcgcgatg tacgaaatag aaaacgtcga gggttatggc
    35221 gaagatatgg atgggctggc ggagccttca gtctatttta atgccgccaa cgggccgtgg
    35281 agaattgctc tggcctatta tcaggaaggg ccggtagatt atagcgcggg taaacgtgga
    35341 acgtggtttg atcgcccgga gctggaggtg cattatcagt tcctcgaaaa cgatgatttc
    35401 agtttcggcc tgaccggcgg tttccgtaat tatggttatc actacgttga tgaaccgggt
    35461 aaagacacgg cgaatatgca gcgctggaaa atcgcgccag actgggatgt gaaactgact
    35521 gacgatttac gtttcaacgg ttggttgtcg atgtataaat ttgccaacga tctgaacacg
    35581 accggttacg ctgatacccg agtcgaaacg gaaacaggcc tgcaatatac cttcaacgaa
    35641 acggttgcct tgcgagtgaa ctattatctc gagcgcggct ttaatatgga cgacagccgc
    35701 aataacggtg agttttctac gcaagaaatt cgcgcctatt tgccgctgac gctcggcaac
    35761 cactcggtga cgccgtatac gcgcattggg ctggatcgct ggagtaactg ggactggcag
    35821 gatgatattg aacgtgaagg ccatgatttt aaccgtgtag gtttatttta cggttatgat
    35881 ttccagaacg ggctttccgt ttcgctggaa tacgcgtttg agtggcagga tcacgacgaa
    35941 ggcgacagcg ataaattcca ttatgcaggt gtcggcgtaa attactcgtt ctgataatgg
    36001 gctaaattgc cggatgcggc acgagtactt tatccgaact ataaatgtag gccggataag
    36061 atgcgctagc atcgcatccg gcattcaggc aaggtagctg gtatttattt cagcgtcata
    36121 tgcgtggcaa cggtaatatt ctgtggtgac ggttttccag aaattaagcg gaataataac
    36181 tcacagcttt gttgacctaa ctcctgtgtt gggatatcaa taccgcctgg cgcaggcgtt
    36241 aaaataaacg acagcgtttc attactataa cccaccaccg ctaactgctg cggaatagca
    36301 atatttttct ctgctgccgc acgataaata ctcattaatt tcaggctgtc agtagcaaac
    36361 actgcctcag gcaacggtga ctggcttaat aattcccgcg cggcttgcag tgctgtttca
    36421 tgggtataac cgccgtcaac aatccattca tcacgcactg caatattatg cgcagccagg
    36481 ctctgcttat aaccatttac ccgatccact gaaacatgga catcaagcgg agcatgcagg
    36541 caggcaatat tttgatgccc actttcaatt aatgcatcgg tcaacgcaat gctgtcgcca
    36601 aaattatcgg tatcgacaga ataaacatgg gcatattgac cttcaacttt gccaatcacc
    36661 acgacaggaa tatcgtattt atcaagttgg gcaaaaaatg actcatccgc tggcgaactt
    36721 agcataataa tgcctttaat cattttctgc ttaattttgc tttcacattt ttgtaagtct
    36781 tctgccggat tgtgcgacgt ctgcaatatc acgtcgaaac cttcctcttc agctttggcg
    36841 gtgatggcat gcaaaacttc agagaaaaac ggattacccg ccgtagtttt ggtcgaacgg
    36901 gtagaaatca ccataatggc atcaaaaccc gaagaggtca gcgcgcgggc tagcttgttt
    36961 ggctgatact gtaattcttc aatggcccgt aacacttttt cgcgcgcttc cggggagata
    37021 ttagtttgct tattcagcac gcgtgatacg gtggattttg atacgcctgc aaccctggca
    37081 atatcataaa tagtagggga cataggtcag gaactccgtc cgaacgtgaa tgctgcacat
    37141 cttatggagt tcgcccgtcg atgacaacac gggcagttga taatcaatgg cctggccccc
    37201 acattcatat ccttacgaat gatttttttc tggtcttcag agcagcacag gacagcaatg
    37261 aagcgactta aaaatgaact taatgcgctg gtgaatcggg gtgtcgacag acatctgcgc
    37321 ctcgccgtaa ccggactaag ccgcagcggc aaaacagcgt ttatcactgc gatggtcaat
    37381 cagttgctca atattcacgc cggagcacgc ttgccgctgt taagcgcggt gcgtgaagag
    37441 cgcctgctgg gcgtgaaacg cattccccag cgtgactttg gcattccgcg cttcacctat
    37501 gatgaagggc tggcgcagtt atatggcgat ccacccgcct ggccaacgcc aacgcgcggc
    37561 gtcagtgaaa tccgcctggc gctacgtttt aaatcgaacg attcgctgct acgccacttc
    37621 aaggacacct ccacgctgta tctggagatt gtggattatc ccggcgaatg gttgctcgac
    37681 ctgccgatgc tggcgcagga ctatttaagc tggtcacgcc agatgacggg cttactcaat
    37741 ggtcagcgcg gcgaatggtc ggtcaaatgg cgaatgatgt gcgaagggct ggacccgcta
    37801 gcacctgccg acgaaaaccg gctggcagac attgccgccg cgtggaccga ttatctccac
    37861 cactgtaaac agcaggggct gcactttatt cagccagggc gctttgtctt gccaggagat
    37921 atggcaggtg cgcccgcgct gcaattcttc ccgtggccgg atgtcgatac ctggggcgag
    37981 tccaaactgg cgcaggccga taagcacacc aatgccggaa tgctgcgcga gcggtttaat
    38041 tattactgcg agaaggtggt gaaggggttc tataagaatc attttctgcg ctttgaccgc
    38101 cagattgtgc tggttgattg cctgcaacct ctcaacagtg ggccacaggc atttaatgat
    38161 atgcgtctgg cactgacgca gctgatgcaa agtttccact acgggcagcg taccttgttc
    38221 cggcgtttgt tttcgccggt tatcgataag ctattgtttg ctgccactaa agcggaccat
    38281 gtgaccatcg atcagcacgc caatatggtt tcattactgc aacaactgat tcaggatgcc
    38341 tggcaaaatg cggcgtttga agggattagt atggattgcc tggggctggc ctcggtgcag
    38401 gcgaccacca gcggcattat tgatgttaac ggtgagaaaa tcccggcgct acgcggtaat
    38461 cgactcagcg atggcgcacc gctcactgtt tatcctggcg aagttcccgc tcgtttgccc
    38521 ggtcaggcgt tctgggataa gcaaggcttc cagtttgagg cgtttcgtcc gcaggtgatg
    38581 gatgtcgaca aaccgctgcc gcatattcgc cttgatgccg cgcttgaatt tttaatagga
    38641 gataaattgc gatgaccgaa ccgttaaaac cacgtattga tttcgacggt ccgctggagg
    38701 tcgatcagaa tccaaaattc agggcgcagc agacctttga cgaaaatcag gcgcaaaatt
    38761 ttgccccggc cacgctcgat gaagcgcctg aagaagaggg gcaagttgaa gcggtaatgg
    38821 atgcagcgtt acgtccgaaa cgcagcctgt ggcgcaaaat ggtgatgggc gggctggctc
    38881 tgtttggcgc aagcgttgtc gggcagggtg tacagtggac aatgaatgcc tggcaaactc
    38941 aggactgggt ggcgctgggt ggatgtgccg ctggggcatt gattatcggc gctggcgtag
    39001 gttctgtggt aacagagtgg cggcgcttat ggcgcttgcg acagcgcgcc catgaacgcg
    39061 acgaagcgcg cgatttgttg cacagccacg gcacgggcaa aggccgcgca ttttgcgaaa
    39121 aactggcgca gcaggcgggt attgatcagt ctcatccagc gctgcaacgc tggtatgcct
    39181 caatccatga aacgcaaaac gaccgtgaag tggtcagttt gtatgcgcat ttggtccagc
    39241 cagttttaga tgcccaggcg cggcgcgaaa tcagccgttc ggcggcggaa tcaacgttga
    39301 tgattgcggt cagcccgctg gcgttggtcg atatggcgtt tatcgcctgg cgcaatctgc
    39361 gtttaattaa tcgcatcgcc acgctgtatg gcattgaact ggggtattac agccgtttgc
    39421 gtctgtttaa gctggtattg ctgaatatcg cttttgccgg agccagcgaa ctggtgcgcg
    39481 aagtggggat ggactggatg tcgcaagatc tcgctgctcg tttgtctacc cgcgcagctc
    39541 agggaattgg tgctggactt ctgacggcac gactcgggat taaagctatg gagctttgcc
    39601 gcccgctgcc gtggattgac gatgacaaac ctcgcctcgg ggatttccgt cgtcagctta
    39661 tcggtcaggt gaaagaaacg ctgcaaaaag gcaaaacgcc cagcgaaaaa taatgcaata
    39721 tcgggtgctg accggatatc tttacgccga agtgcccgtt tttccgtctt tgtgtcaatg
    39781 attgttgaca gaaaccttcc tgctatccaa atagtgtcat atcatcatat taattgttct
    39841 tttttcaggt gaaggttccc atgcgtctgg aagtcttttg tgaagaccga ctcggtctga
    39901 cccgcgaatt actcgatcta ctcgtgctaa gaggcattga tttacgcggt attgagattg
    39961 atcccattgg gcgaatctac ctcaattttg ctgaactgga gtttgagagt ttcagcagtc
    40021 tgatggccga aatacgccgt attgcaggtg ttaccgatgt gcgtactgtc ccgtggatgc
    40081 cttccgaacg tgagcatctg gcgttgagcg cgttactgga ggcgttgccc gaacctgtgc
    40141 tctctgtcga tatgaaaagc aaagtggata tggcgaaccc ggcgagctgt cagctttttg
    40201 ggcaaaaatt ggatcgcctg cgcaaccata ccgccgcaca attgattaac ggctttaatt
    40261 ttttacgttg gctggaaagc gaaccgcaag attcccataa cgagcatgtc gttattaatg
    40321 ggcagaattt cctgatggag attacgcctg tttatcttca ggatgaaaat gatcaacacg
    40381 tcctgaccgg tgcggtggtg atgttgcgat caacgattcg tatgggccgc cagttgcaaa
    40441 atgtcgcagc ccaggacgtc agcgccttta gtcaaattgt cgccgtcagc ccgaaaatga
    40501 agcacgttgt cgaacaggcg cagaaactgg cgatgctaag cgcgccgctg ctgattacgg
    40561 gtgacacagg tacaggtaaa gatctctttg cctacgcctg ccatcaggca agccccagag
    40621 cgggcaaacc ttacctggcg ctgaactgtg cgtctatacc ggaagatgcg gtcgagagtg
    40681 aactgtttgg tcatgctccg gaagggaaga aaggattctt tgagcaggcg aacggtggtt
    40741 cggtgctgtt ggatgaaata ggtgagatgt caccacggat gcaggcgaaa ttactgcgtt
    40801 tccttaatga tggcactttc cgtcgggttg gcgaagacca tgaggtgcat gtcgatgtgc
    40861 gggtgatttg cgctacgcag aagaatctgg tcgaactggt gcaaaaaggc atgttccgtg
    40921 aagatctcta ttatcgtctg aacgtgttga cgctcaatct gccgccgcta cgtgactgtc
    40981 cgcaggacat catgccgtta actgagctgt tcgtcgcccg ctttgccgac gagcagggcg
    41041 tgccgcgtcc gaaactggcc gctgacctga atactgtact tacgcgttat gcgtggccgg
    41101 gaaatgtgcg gcagttaaag aacgctatct atcgcgcact gacacaactg gacggttatg
    41161 agctgcgtcc acaggatatt ttgttgccgg attatgacgc cgcaacggta gccgtgggcg
    41221 aagatgcgat ggaaggttcg ctggacgaaa tcaccagccg ttttgaacgc tcggtattaa
    41281 cccagcttta tcgcaattat cccagcacgc gcaaactggc aaaacgtctc ggcgtttcac
    41341 ataccgcgat tgccaataag ttgcgggaat atggtctgag tcagaagaag aacgaagagt
    41401 aagcgcgaat atgcctgatg gtgcaacacc atcaggcata ttaaattatg ctttcagtac
    41461 agccagagct gcttcgtaat ccggctcggt ggtgatttca tccaccagct ggctgaaaat
    41521 cacattgtca ttttcgtcaa taaccacaac ggcacgtgct gccagacctt tcagtgggcc
    41581 atcagcaatt gccacaccgt aggcttgcag gaattcagcg ttacggaaag tggagagggt
    41641 gataacgttg ttcagacctt ctgcgccgca gaaacgagac tgggcgaacg gcagatcggc
    41701 agaaatacac agcacaacgg tgttgtcgat ctcggttgcc agttggttaa acttacgtac
    41761 tgatgcggcg caaactccgg tatcaatact cgggaaaatg ttcagcactt tgcgtttacc
    41821 cgcaaactga ccgagggtga cgtcagacag atcttttgcc acgagagtaa aagtctgcgc
    41881 tttgctaccc gcctgcggga tggaattggc gactgtaacc gggttgccct ggaaatgaac
    41941 ggtttgtgac atgattatct ttcctgttta catatagtta acgtcacacc tagtttatgc
    42001 taactgtcaa taacacagca aacgctattt gcgcttaatc cgcagaccac cgcgacaaca
    42061 aggagtaaag atgagaaccg ttaaggtatt tgaggaagcc tggcccttac ataccccgtt
    42121 tgtgattgcc cggggaagtc gcagtgaagc gcgcgtggtg gtggttgaac tggaagaaga
    42181 gggtattaaa ggcaccggcg aatgcacgcc gtatccgcgt tatggggaaa gtgatgcctc
    42241 ggtaatggcg caaattatga gcgtcgtacc gcaattagag aaagggctga cacgggagga
    42301 gttgcaaaaa attctccctg ccggcgcagc acgtaatgcg ctggattgtg cattgtggga
    42361 tctggcggcg cgaaaacagc agcaatcgct ggctgatttg atcggcataa cgcttcctgg
    42421 gacagttatc actgcacaga cggttgtcat cggtacgcct gatcagatgg ccaatagtgc
    42481 atcaacactc tggcaggcag gcgcgaaatt actgaaagtg aagctggata accatcttat
    42541 cagtgagcgg atggtggcaa ttcgcaccgc tgtgcccgat gccacgctga tcgttgatgc
    42601 aaatgaatcc tggcgtgcag aagggttggc ggcgcgttgc cagctattgg cggatttagg
    42661 cgttgcgatg cttgaacaac cgcttcctgc gcaggacgat gcggcactgg agaattttat
    42721 tcatccgttg ccgatttgtg ctgatgaaag ttgtcatact cgtagcaatc tgaaggcgct
    42781 gaaagggcgc tatgagatgg ttaacattaa gctcgataaa accgggggtc tgacggaagc
    42841 gctggcgctg gcgactgaag cacgtgcaca aggttttcgt ctgatgctgg gctgcatgtt
    42901 gtgtacctct cgtgccatta gcgccgcttt accgctggtg ccgcaggtca gtttcgccga
    42961 tcttgacgga ccgacctggc tggcggtaga tgtggaaccg gcgcttcagt tcacgacggg
    43021 cgaattgcat ctttaggatg ccagcgcagc aagtttgcca tcgcaaagag atatttttcg
    43081 ctggcttcgt cggaggagat gggcggaaac tctgcggtga tgcaatgcaa gttgagatct
    43141 gcacaccaac tgccgaaaga gccaggcgtt tcatagccga cgctggttac cagaggcaat
    43201 tcaaacgcct gggacagcca ttcgcctaat tcgctgtgtc tgggatcttc aatacaagcc
    43261 agtggatcgt ggaatgagac cacccaggca ggttgaatgc ggtgtataag ctggcacagc
    43321 gcctgggttt caggttcgga gccaggtttg tcacctgtca gtaatacgac atcacgttct
    43381 tcagcggcgc tgttccagcg ataaaccgtt tcaccttcct tccagttcgc cgccggaaag
    43441 tttctgttta aatccacacc attagcattg gcccgtaacc ctaactggca gccgtcagga
    43501 ttcacacaca gcaccacatg atggcggcgc aaagaaggtg tcaaagtccg cagagcacag
    43561 gagagcgtga cgaccgaaga gttttcatca ccgtgagtgc cagccagaat caaaccactt
    43621 tcgcgactgg ctgcaggggc aggaaaccag attaacggcg cgcccaataa tgaacgtccg
    43681 taatgttctg ttccgggcgg aaatgcgccg cgttcggcgc gtgggcgggt tacggtcatg
    43741 atcgtctctg gttcaggaag tattacaggc agtgttgtgc aaattttgcg gattatcaaa
    43801 tccttttctt caacgctatg tcatcgatct tgtatcactt ttttgccgga agttgtttgc
    43861 attttcattg atgtgcagat attagaattg tcgtaatgtt atatacatgg cgtattttat
    43921 tcttactggt tctacacatg ttaatcgcat cggattgatc agttcacctt aataagttta
    43981 ataaatcgta ctgcacatag tacagtacga tggtagttat gtaattgatt tacatgaaga
    44041 atggcttatc accatcagta ccgggttgat tgatgcctat attctcacca atgtgtttct
    44101 caggtttatc gatgatatct gtgattaatg ctgcgacact cttacgtgat acgatcgtac
    44161 ctttgaatgg ttcattgcgt gaggtaagct cataatcaat aatatcttca tcagtcagcc
    44221 aggcagggcg aagaattgta tattccagtc cagaagcctc aattgcatcc gcagccctgc
    44281 ggaatggttt taggggctca ccaataactg cattattcca ttcgccaaat tttcctggaa
    44341 cttcatcata aataccgagg gaaagaacaa agatcagacg tttaacatca caggctttca
    44401 tcgcagcgat cactgaattt gcctgaatat ctaaatcttc ccccgtaagg ttggcataca
    44461 caacgtcctg tccctgcatg gcctgcttca gtgctgcatg attcagtaca tctcccataa
    44521 taatcttgct attagttgga tagggtttgt ggatttttgc tggctgacgg gcaaataaag
    44581 tttgtttaat ggtttgttta tctgccagtt gattaatcac atggcgggct atctgaccgc
    44641 cagcaccaag aatcagtaca tttttcataa tatttctcat gaaacttatc ccagatgggt
    44701 gttgaaaaaa ggaataactt tttccagcgc ctcctgaact gcttccggtt tgtcatacag
    44761 ttcataatgt gagaaatcgg gtaatgaaac taactgacga tcctgcgaaa ccatcgcccg
    44821 gccatagact tccagtccat cacgataagc accgaaggca ccgacttttt caccaactac
    44881 taccatcacc ggctgagtca gtaacacttc agtaaatgcg aaggcatccc aggccagcgt
    44941 tttctgtgca tgtgagaaca acatttttgt cgcacctccc ggttgttgcc cgcgtggtgt
    45001 tttatagtag tcagttgctt cataaacatc gcgttcggtc agtccatggg attttgctgc
    45061 atccaggctg gcgggaagta attcgttgat ctgtaattct ccaccacgag cctctttggt
    45121 tcgttgcgac gccattgcat tcaatgcgcc aatcggatcg taattgctga aaccttcccg
    45181 aaaaagacgc ccgatattaa cacccgtaat actcactact gctttaattc gtttttctgt
    45241 cagcgcggca ttaatggcat agccaccacc cccacacact cccagcacgc caatacgttc
    45301 agcattaaca tatggcagag ttactgcata atcgataaca cggctaatat cctctacgcg
    45361 ctgtgtcgga tcttctatcc agcgaggctc cccaccagat tctccctgga aactggcgtc
    45421 ataagcgaga acgagatagc ctttttcagc caatgctttg ccatagatat tgccagacgt
    45481 ctgctcctta caactgccaa aaggatggac gctgataatc gccggatact gttttgactc
    45541 atcaaaattt ggtggaaact gaatattggc agcaatatgc caggccatat cactattgcg
    45601 gatttgaata tgtttcatta cagagtttcc tcaaagaacg gagctaatac tgaaattgct
    45661 tcagccacat aggctttgcc gtcataaaga tccatatggt ttgcgccttc aacaatgtga
    45721 tagcgtttat cctggcttga tgcccgatcg tacaggtcgt cactcatcca tttgctccct
    45781 gcctggctgc ccgccacaat ctgcatcggc tgagtcaggt acacttccgc catatggtaa
    45841 gcatcatagg taataatctg gttaaggctg cgcaaagtag cgtaacccgg tgctgttgga
    45901 tactgcgcgc gaggggtgtg gtaatactcc caggcctgac gcagttcttc gttcggcgca
    45961 tcggactcct tcattggtgc cagtggcata acggcatatt ctccgctgct aatatcgctg
    46021 gttcgggcgt ttgaacccgc ttcaacgtat ggcagggcat caatagattt cacattgttt
    46081 tcccaaccat tacggaacat cgaaccaata ttgaccgcac taacggtacc gatggccttg
    46141 atgcggcgat cctgaattgc agcattggct gtatatcctg caccggcaca aattcccatc
    46201 gcaccaattc gggtattgtc gacataagaa agcgttgtca ggtaatcaat cacggcactg
    46261 atgtcttcag tacgaatgta tgggttttct aactgacgcg gctcgccgcc actttcacct
    46321 tgataagatg cgtcataagc aatagtgaca aatccctttt ccgccagttt tttggcatag
    46381 gttccggccg tttgttcttt aacgccccca cctgggtgag ataccacaat tgcctgatac
    46441 tgacgggttt catcaaattt tggagggaaa tagatcactg cagacaaaga gatggttgga
    46501 ttattgctgt tagtgaagct gactttatta ttcatcattc cgttcctcat ggagttgtcg
    46561 gttcgtttta acggttggtg atatcactat agatattgat cattaagttg attagaccca
    46621 aaatcatgat tagacctatc atttaaatga ttaatagatt gcatggagat actgaatgaa
    46681 gcgtgaagaa atcgctgatc tgatggcgtt tgtcgtcgtt gcagaggagc gtagcttcac
    46741 tcgtgcagca gcccgcctga gcatggcgca gtcagcttta agccagatag tgcgtcgtat
    46801 agaagaacga ttgggattgc ggcttctgac gcgaaccacg cgcagcgttg ttccaactga
    46861 agcgggcgag catcttttgt ctgttcttgg ccctatgttg catgacatag attcagccct
    46921 ggcatccctg agcgagctgc agaaccgccc atccgggaca atacgtatta ctactgtaga
    46981 acatgcagca aaaacgatat tgttaccagc aatgcgcaca ttcctgaaat cgcatcctga
    47041 aattgatatt cagctcacca ttgattatgg tttgaccgat gtcgtttctg aacgttttga
    47101 tgcaggcgtc cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc
    47161 agatatacca atggctattg ttggctcacc ggattatttt tctcgccgaa gtgttccaac
    47221 gtcagtgtca caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac
    47281 agcaaatcgc tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca
    47341 gcttttactg aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc
    47401 gtatctacct tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt
    47461 ggataaattc acaccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc
    47521 tggctcggca ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta
    47581 cttgctgata cattaattta atttctctct taacgtattc tcagttcctt tcaacgtttt
    47641 ggttattttt tattcttcgt acaatggcga cagatgctga ttttgatacc gaaaactggt
    47701 ttgaacgtgc gaagcctgaa cgtagtgttc ggtgttctat gtgctttgcc gcattttggc
    47761 g
//
LOCUS       DAPGWD010000020        46114 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPGWD010000020 DAPGWD010000000
VERSION     DAPGWD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.020"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            53..2281
                     /gene="crfC"
                     /locus_tag="RHX88_001065"
     CDS             53..2281
                     /gene="crfC"
                     /locus_tag="RHX88_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDV2229790.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHDDLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQARGYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2278..3156
                     /locus_tag="RHX88_001066"
     CDS             2278..3156
                     /locus_tag="RHX88_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDV2229791.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQVGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGVFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            3420..4922
                     /gene="proP"
                     /locus_tag="RHX88_001067"
     CDS             3420..4922
                     /gene="proP"
                     /locus_tag="RHX88_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDV2229792.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5034..5123
                     /gene="pmrR"
                     /locus_tag="RHX88_001068"
     CDS             5034..5123
                     /gene="pmrR"
                     /locus_tag="RHX88_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDV2229793.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5099..6199)
                     /gene="pmrB"
                     /locus_tag="RHX88_001069"
     CDS             complement(5099..6199)
                     /gene="pmrB"
                     /locus_tag="RHX88_001069"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDV2229794.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6200..6868)
                     /gene="basR"
                     /locus_tag="RHX88_001070"
     CDS             complement(6200..6868)
                     /gene="basR"
                     /locus_tag="RHX88_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDV2229795.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6865..8508)
                     /gene="eptA"
                     /locus_tag="RHX88_001071"
     CDS             complement(6865..8508)
                     /gene="eptA"
                     /locus_tag="RHX88_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDV2229796.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8612..9949)
                     /gene="adiC"
                     /locus_tag="RHX88_001072"
     CDS             complement(8612..9949)
                     /gene="adiC"
                     /locus_tag="RHX88_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDV2229797.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10086..10847)
                     /gene="adiY"
                     /locus_tag="RHX88_001073"
     CDS             complement(10086..10847)
                     /gene="adiY"
                     /locus_tag="RHX88_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDV2229798.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11172..13439)
                     /gene="adiA"
                     /locus_tag="RHX88_001074"
     CDS             complement(11172..13439)
                     /gene="adiA"
                     /locus_tag="RHX88_001074"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDV2229799.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEIQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13638..14546)
                     /gene="melR"
                     /locus_tag="RHX88_001075"
     CDS             complement(13638..14546)
                     /gene="melR"
                     /locus_tag="RHX88_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDV2229800.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14829..16184
                     /gene="melA"
                     /locus_tag="RHX88_001076"
     CDS             14829..16184
                     /gene="melA"
                     /locus_tag="RHX88_001076"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDV2229801.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELECKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16287..17708
                     /gene="melB"
                     /locus_tag="RHX88_001077"
     CDS             16287..17708
                     /gene="melB"
                     /locus_tag="RHX88_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDV2229802.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(17847..18476)
                     /locus_tag="RHX88_001078"
     CDS             complement(17847..18476)
                     /locus_tag="RHX88_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDV2229803.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(18599..20245)
                     /gene="fumB"
                     /locus_tag="RHX88_001079"
     CDS             complement(18599..20245)
                     /gene="fumB"
                     /locus_tag="RHX88_001079"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV2229804.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(20323..21663)
                     /gene="dcuB"
                     /locus_tag="RHX88_001080"
     CDS             complement(20323..21663)
                     /gene="dcuB"
                     /locus_tag="RHX88_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDV2229805.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(22234..22953)
                     /gene="dcuR"
                     /locus_tag="RHX88_001081"
     CDS             complement(22234..22953)
                     /gene="dcuR"
                     /locus_tag="RHX88_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDV2229806.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(22950..24581)
                     /gene="dcuS"
                     /locus_tag="RHX88_001082"
     CDS             complement(22950..24581)
                     /gene="dcuS"
                     /locus_tag="RHX88_001082"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDV2229807.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAMARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            24762..24992
                     /locus_tag="RHX88_001083"
     CDS             24762..24992
                     /locus_tag="RHX88_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDV2229808.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            25004..25276
                     /locus_tag="RHX88_001084"
     CDS             25004..25276
                     /locus_tag="RHX88_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2229809.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            25503..25799
                     /gene="ghoS"
                     /locus_tag="RHX88_001085"
     CDS             25503..25799
                     /gene="ghoS"
                     /locus_tag="RHX88_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV2229810.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            25827..26000
                     /gene="ghoT"
                     /locus_tag="RHX88_001086"
     CDS             25827..26000
                     /gene="ghoT"
                     /locus_tag="RHX88_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDV2229811.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(26119..27636)
                     /gene="lysS"
                     /locus_tag="RHX88_001087"
     CDS             complement(26119..27636)
                     /gene="lysS"
                     /locus_tag="RHX88_001087"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV2229812.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(27873..29330)
                     /gene="dtpC"
                     /locus_tag="RHX88_001088"
     CDS             complement(27873..29330)
                     /gene="dtpC"
                     /locus_tag="RHX88_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDV2229813.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(29389..31536)
                     /gene="cadA"
                     /locus_tag="RHX88_001089"
     CDS             complement(29389..31536)
                     /gene="cadA"
                     /locus_tag="RHX88_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDV2229814.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(31616..32950)
                     /gene="cadB"
                     /locus_tag="RHX88_001090"
     CDS             complement(31616..32950)
                     /gene="cadB"
                     /locus_tag="RHX88_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDV2229815.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(33316..34854)
                     /gene="cadC"
                     /locus_tag="RHX88_001091"
     CDS             complement(33316..34854)
                     /gene="cadC"
                     /locus_tag="RHX88_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDV2229816.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(35471..35546)
                     /locus_tag="RHX88_001092"
     tRNA            complement(35471..35546)
                     /locus_tag="RHX88_001092"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35511..35513),aa:Phe,seq:gaa)
     gene            complement(35653..36228)
                     /locus_tag="RHX88_001093"
     CDS             complement(35653..36228)
                     /locus_tag="RHX88_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2229817.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(36265..37962)
                     /gene="dsbD"
                     /locus_tag="RHX88_001094"
     CDS             complement(36265..37962)
                     /gene="dsbD"
                     /locus_tag="RHX88_001094"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDV2229818.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(37938..38276)
                     /gene="cutA"
                     /locus_tag="RHX88_001095"
     CDS             complement(37938..38276)
                     /gene="cutA"
                     /locus_tag="RHX88_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDV2229819.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(38392..39693)
                     /gene="dcuA"
                     /locus_tag="RHX88_001096"
     CDS             complement(38392..39693)
                     /gene="dcuA"
                     /locus_tag="RHX88_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDV2229820.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(39811..41247)
                     /gene="aspA"
                     /locus_tag="RHX88_001097"
     CDS             complement(39811..41247)
                     /gene="aspA"
                     /locus_tag="RHX88_001097"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDV2229821.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            41584..42060
                     /gene="fxsA"
                     /locus_tag="RHX88_001098"
     CDS             41584..42060
                     /gene="fxsA"
                     /locus_tag="RHX88_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDV2229822.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(42076..43332)
                     /gene="yjeH"
                     /locus_tag="RHX88_001099"
     CDS             complement(42076..43332)
                     /gene="yjeH"
                     /locus_tag="RHX88_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDV2229823.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWVGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            43608..43901
                     /locus_tag="RHX88_001100"
     CDS             43608..43901
                     /locus_tag="RHX88_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDV2229824.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            43945..45591
                     /gene="groL"
                     /locus_tag="RHX88_001101"
     CDS             43945..45591
                     /gene="groL"
                     /locus_tag="RHX88_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDV2229825.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            45729..46082
                     /locus_tag="RHX88_001102"
     CDS             45729..46082
                     /locus_tag="RHX88_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDV2229826.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCLN"
BASE COUNT        11683 a        11118 c        11692 g        11621 t
ORIGIN      
        1 aacaatctgg ctaaataaaa gtaacaaaat ttgctttaag gaagaatttt ctatgtacac
       61 acagaccctg tatgagttaa gtcaggaggc tgaacgcctg ttacagcttt ctcgccaaca
      121 gttgcagtta ctggaaaaaa tgcctctctc tgtacccgga gacgacgcgc cacaactggc
      181 tttaccctgg agtcagccta atatcgccga acgtcacgcg atgctgaata atgagttgcg
      241 taaaatttcc cgactggaaa tggtgctggc aattgtcggt accatgaaag cagggaaatc
      301 aaccaccatt aatgccattg ttggtacgga ggttctgcct aatcgtaatc gcccaatgac
      361 tgcgctgccg acgcttattc gccatacgcc cgggcaaaag gaaccggtac tgcatttttc
      421 acatgtcgcg ccaatcgatt gtttaattca acaattacaa cagcgcctgc gtgattgcga
      481 tattaagcat ctgaccgatg tgctggaaat agataaagat atgcgtgcgc ttatgcagcg
      541 gatcgaaaat ggcgtcgctt tcgaaaaata ttatctgggt gcccagccta tttttcattg
      601 tctgaaaagt ttgaatgatt tagtgcgact ggcgaaggcg ctggacgtcg attttccttt
      661 ttctgcttac gccgccattg agcatattcc cgtgattgaa gtggagtttg tccatctggc
      721 ggggctggag agttatcccg gtcagttgac gttactggat acccccgggc caaatgaagc
      781 cgggcaaccg catctgcaaa aaatgcttaa ccagcagctg gcacgcgcct cggcggtact
      841 ggcggtgctg gattatacgc aactgaaatc gatctccgat gaagaggtcc gtgaggcgat
      901 tttggcggtg gggcaatcgg tgccgctgta tgtgctggtc aataagttcg atcaacagga
      961 tcgtaacagt gacgacgccg accaggtgcg ggcactgatt tccgggacgc tgatgaaagg
     1021 ctgtattacg ccacagcaga tatttccggt gtcgtcgatg tggggctacc tggcgaatcg
     1081 agcgcgccat gagttagcca acaacggtaa gttaccagcg ccagagcaac aacgctgggt
     1141 ggaagatttt gcccatgctg cgctcggcag gcgctggcgt catgacgatc tggcggacct
     1201 cgaacatatt cgtcatgctg ccgatcagtt gtgggaagat tcgctgttcg cccagccaat
     1261 tcaggcgttg cttcatgccg cttacgctaa cgcctcgttg tatgctctgc gatctgctgc
     1321 gcataaactg ttgaattacg cgcagcaggc gcggggatac ctggattttc gtgcgcacgg
     1381 gttaaacgtc gcttgtgaac aattgcggca aaatatccat caggtcgaag aaagtttgca
     1441 gctattgcaa ctcaatcagg cgcaggtgag cggcgagatt aaacatgaaa tcgagctggc
     1501 cctgacctcc gccaaccact ttctgcgtca acaacaagat gcgctgaatg cgcagttagc
     1561 cgccttgttt caggatgatt cggagccgtt aagcgagatg cgtacccgct gtgagacact
     1621 gttacagacg gcgcagaaca ccatcagtcg cgactttacg ctgcgttttg ccgagctgga
     1681 atccaccctt tgccgggtgt taaccgatgt tattcgcccc attgagcaac aagtcaaaat
     1741 ggaattgagc gagtcagggt ttcgtcctgg gtttcatttt cctgtttttc acggcgtagt
     1801 tccccacttc aacactcgcc agctgttcag tgcagtcatt tcgcgccagg atgcaacgga
     1861 cgagcagagc acgcgtttag gcgttgtgcg tgagactttt tcgcgctggt tgaatcagcc
     1921 cgactgggga cggggaaatg agaaatcccc gacagaaacg gttgattaca gtgtgttgca
     1981 acgagcatta agcgcagaag tcgatcttta ttgccaacaa atggctaaag ttctggcaga
     2041 gcaggtcgat gaatctgtta cggcaggcat gaatactttt ttcgctgagt tcgcttcatg
     2101 tttgacggaa ttacagacgc gtttacgcga aagcctggct ctgcgtcaac aaaatgaatc
     2161 ggtggtcagg ctgatgcagc agcaattgca gcagactgtg atgactcacg gctggattta
     2221 caccgacgcc cagctgttac gcgatgatat tcaaacactt ttcacggcag aacgatattg
     2281 accaagacgt tactggacgg ccccggtcgc gtgctggagt cggtttatcc ccgcttttta
     2341 gtggatctgg cgcagggcga tgatgcccgc cttccacaag cccatcagca gcagtttcgt
     2401 gaacgactga tgcaggaact tctttcgcgt gtgcagcttc agacatggac gaacggcggc
     2461 atgttaaatg cgccgcttag cctgcgactg acaatggtgg aaaaactggc gtcgatgctg
     2521 gatcccggtc atctggcgct gacgcagatc gcgcagcatc tggcgctgct gcaaaaaatg
     2581 gatcaccgcc agcactctgc tttcccggag ctcccccagc aaattgccgc cttgtatgag
     2641 tggttttcag cccgttgtcg ctggaaggaa aaggcattaa cgcaacgagg cctactggtg
     2701 caggtgggtg aacagagcga gcaaattttt acccgctggc gtgctggggc gtataacgcc
     2761 tggtcgttgc ctgggcgctg ttttatcgtt ctcgaagagt tgcgttgggg ggtatttggc
     2821 gatgcctgtc gtctgggaag cccgcaagcg gtggcgttgt tgctgggtga tttgcgcgtg
     2881 aaagcgacac aacatctggc agagagtatc aatgcggccc cgaccacgcg tcactattac
     2941 catcagtggt ttgcctcttc gaccgttccg acgggcgggg atcatgctga ttttttaagt
     3001 tggctgggaa agtggaccac ggcagataaa caacccgttt gctggtcagt gacccaacgc
     3061 tggcaaactg tcgcgctggg gatgccacga ctctgttcag cgcagcgtct ggcgggggca
     3121 atggtcgagg aaatcttctc tgtaaatttg gcgtaaataa tcagttacat caatgagtcc
     3181 taaacgaaat ccatgtgtga agttgatcac aaatttaaac actggtaggg taaaaaggtc
     3241 attaactgcc caattcaggc gtcaactggt ttgattgtac attccttaac cggagggtgt
     3301 aagcaaaccc gctacgcttg ttacagagat tgcatcctgc aattcccgct ccccttttgc
     3361 ggccgtcgcg ctgatttttc tggcgtttgc ggaaatgggc caactctgcg aggaaagcta
     3421 tgctgaaaag gaaaaaagta aaaccgatta cccttcgtga tgtcaccatt attgatgacg
     3481 gtaaactgcg taaagccatt accgcagcat cactgggtaa tgcaatggaa tggtttgatt
     3541 ttggtgttta tggttttgtt gcttacgcat taggtaaagt ttttttcccg ggggctgacc
     3601 ccagcgtgca gatggttgct gcacttgcca cgttctccgt tccctttctg attcgaccgc
     3661 ttggcggact cttctttggt atgttgggcg ataaatatgg tcgccagaag atcctcgcta
     3721 tcactattgt gattatgtcg atcagtacgt tctgtattgg cttaataccg tcctacgaca
     3781 cgattggtat ttgggcaccg attctgctgt tgatctgtaa gatggcacaa ggtttctcgg
     3841 tcggcggtga atataccggg gcgtcgatat ttgttgcgga atactcccct gaccgtaaac
     3901 gtggctttat gggcagctgg ctggactttg gttctattgc cgggtttgtg ctgggtgctg
     3961 gcgtagtggt gttaatttcg accattgtcg gcgaagcgaa cttcctcgac tggggctggc
     4021 gtattccgtt ctttattgct ctgccgttag ggattatcgg gctttacctg cgccatgcgc
     4081 tggaagaaac tccggcgttc cagcagcatg tcgataaact ggaacagggc gaccgcgaag
     4141 gtttgcagga tggcccgaaa gtctcgttta aagagattgc caccaaacac tggcgcagcc
     4201 tgttgacatg tattggtctg gtaattgcca ccaacgtgac ttactacatg ttgctgacct
     4261 atatgccgag ttatttgtcg cataacctgc attactccga agaccacggg gtgctgatta
     4321 ttatcgccat tatgatcggt atgctgtttg tccagccggt gatgggcttg ctgagtgacc
     4381 gttttggccg tcgtccgttt gtgctacttg gtagtgttgc cctgtttgtg ttggcgatcc
     4441 cggcgtttat tctgattaac agtaacgtca tcggcctgat ttttgccggg ttactgatgc
     4501 tggcggtgat ccttaactgc tttacgggcg ttatggcttc taccttgcca gcgatgttcc
     4561 cgacgcatat ccgttacagc gcgctggcgg cggcatttaa tatttcggtg ctggttgccg
     4621 gtctgacgcc aacactggcg gcctggctgg tcgaaagctc gcagaatctg atgatgcctg
     4681 cctattacct gatggtagtg gcggtgattg gtttaatcac cggcgtaacc atgaaagaga
     4741 cggcaaatcg tccgttgaaa ggtgcgacac cggcggcgtc agatatacag gaagcgaagg
     4801 aaattctcgt cgagcattac gataatatcg agcagaaaat cgataatatt gaccacgaga
     4861 ttgccgattt gcaggcgaaa cgtacccgcc tggtgcagca acatccgcga attgatgaat
     4921 aagctgaaac ggatggcctg atgtgacgct gtcttatcag gccaattgaa ctcttaaggt
     4981 tcacttaatc tctgacgcgc atactctcct ccaggttaac ggaggagagt gcaatgaaaa
     5041 accgtgttta tgaaagttta actaccgtgt tcagcgtgct ggtggtcagc agctttcttt
     5101 atatctggtt tgccacgttc tgatctttct tcagccgtac ccaggcccgc gtgccggaag
     5161 tctcttgccg gttttgcagg aaaaactgcc cgtgatgcaa ctgtgtaatg cggctgacaa
     5221 tacttaaccc cagaccaatc ccgccataac ggctgtccat acgtacaaac gctttactca
     5281 actccccgca tttactctca tcaatacctg gtccttcatc ttcaactgcc atgaccgctc
     5341 cgccatcttc ttgcagctta atcataatgt tgctgccttg cgggctgtaa cgatgggcgt
     5401 tttctaccag gtttcgcaat aacatccgca gcagggttgc atcaccctga acggtgatgt
     5461 cggcggcgct ctctggcaat agcagggttt gctgtcgctg gtcgagcatg gtactgagtt
     5521 cgtcatacga ggggagaatg acatcttcca gcagttttac atgttgataa ttaccggaag
     5581 aaaatgactg tccggcacgc gccagttgca gcagctggga gacgctctcc atcatctgat
     5641 caagccgtgc cactaacggt gctacatcaa tgtgatgcgt tttcgccagc agttccagat
     5701 gcaaacgcac ccccgccagt ggcgttcgca gttcgtgcgc gacgtcagcg gtaaacaacc
     5761 tttcgttatc cagcgtgctg gtcaggcgac tgaccagatc gttcaacgcc gaaaccaccg
     5821 cttcgatttc gagggtggcg ctgtgaatgg caatgggcgt taagttgtcg gcggtgcgcg
     5881 cttccagctc tttttgcagc tccgccagcg ggcgggtgat gcggcgtacc gcctgatagc
     5941 agataaatag cgtcaggctg accataaaga cgccggggac aatcaggctg gcgaccgcct
     6001 cgcggatctc acgcatgatg tggcgatcgt tgttgcgatt gtcgcgtagc gcctgctcaa
     6061 acagctgaat ctgctcggta ctttcatgcc atagccagaa gacgctgatc agctcaaaca
     6121 ccaacaaaat ggccccgatg gtcaatatca gccgttggcg cagcgatatt ggtcggcgca
     6181 gaaaatgcat cagattcaat tagttttcct cattcgcgac cagcatatag ccaaagccgc
     6241 gcacggtgcg gatacgggct ttgcccactt tgtcgcgcag attgtggata tgcacttcca
     6301 gggtgttggt cgagggttca ttgtcccagt tatagatgtc gttgtagaga atttcccgat
     6361 gcaccggact gcctgctttg agcattaacc gtgacagcag agcatattct ttgggcgtca
     6421 gaatcaactc ttcaccgccc atccatacct gacggcgacc catattcagc gtcagattgc
     6481 caacaatcag ctcactttcg ccctgattat tatggcgtcg tagcagggcg cggatacggg
     6541 cgtgtaactc ttccagcgca aaaggcttca ccagatagtc atcggcaccg acatccagcc
     6601 cggcgatttt gtcggtcagc gtatcgcgag cggtgaggat cagtaccggc agggtatatt
     6661 ttttctgccg gatacgggcg agaaaatgca gtccatcttc gtcgggtaac cctaaatcca
     6721 gtaccaccag gctgtaatga ccggcctcaa ggctttgttc cgccatccgc gcggttgtca
     6781 cgccatcgca cgcgtagcct tcagtttgcg ccgccagaat caatccctgc aataacagcg
     6841 tatcgtcttc aacaatcaga attttcattc actcactctc ctgcaagttt gcagaatatc
     6901 atccgcagcc tggtaatact tcgtctcaac gccagttaat cccaatagcg tggagaataa
     6961 attgtcttgt gaatagtgtt gcgtttgcgc ctgtttttgc aggcagttct ggtcaacctg
     7021 ataccgtttt tgataatcct ccgacagcca cagcagcatc ggcacctgtt tttggctatc
     7081 cggggcgatg gcataaggca gaccgtgcag atagatgcca ttttcaccta acgattcacc
     7141 gtggtcagaa agataaacca ggctggtggt aaatttatcc tgatgttctt tcagcagatt
     7201 aatcgcttta tcaacaatat agtcgacgta aaccagcgtg ttgtcgtaag tgttcaccag
     7261 ttgctctttg ctacaggtct ggatctcatt ggtgtcgcag gttggggtaa atttcctgaa
     7321 ctgcggcgga tagcggttgt aataggtcgg accgtggctg ccgatggtgt gtaagacaat
     7381 cacgccatca ccctgcaggt tattgatgta ctcttccagc ccgtggaaca gtacttcgtc
     7441 atagcattcg ccgttgatgc actgaccagg cagattcagc gcggtgacgt tctggtgagg
     7501 cacgcggtcg caggcacctt tacagccgcc atcgttgtca ttccacagca cgttgatgcc
     7561 cgctcgctga atgatatcca gcacgccttc ctggtgctgt gccagctctt ctttgtaatg
     7621 ctcacgcggc atatccgaga acatgcacgg tactgaaact gccgttgccg tgccgcaaga
     7681 tgcggtatta gggaaataga ccacgttatc tttcgccagc cgcgggttag tttcacgcgg
     7741 gtagccgttg agggagaagt tctccgcccg cgaggtttcg ccgacaatca ggatggtcaa
     7801 atttttacgt ttttcgttct gcattaacgg gttgcggtgg gcgtcttcac caattcgcac
     7861 cagcggtaga tttgccagtc gctgatggga gtaccatgac cagctggcaa caatgctgtt
     7921 agaggggctt aaggatttca ccagctcttt gttattgcgg aacaacgagg cgtagtcttt
     7981 ataaaacagt gcggcgacca gcaaaatcag tagtacagaa accagaatat tggctccacg
     8041 gaaaagaaca ctgcgcagac gcgaggtggc aggtttgatt tttatccagc aggcaatcag
     8101 cgcagcaagc acgccgctga atcccagcgt taataacatt tgcggtgtca tcagcgcata
     8161 actttctgcc ggagtggtat caataatatt ggcaatcatc gagcggtcga tgacgatgcc
     8221 gtaagtcatt atgaaatatt gtgcagccgc gccaaccaga ataaacaggc aggccagtgg
     8281 tcgattaagc cataagaaag agcttagtgt caggacaata ttaatcacgc tgaaagcgac
     8341 gaccggcatc gacaagaaaa ccagtacgtt atgcagcgaa tccagcggca gcgcctgcaa
     8401 cacctgttta aaaaaggcaa tattcaggca gatagagata taaaaagcgg ccaacaatag
     8461 ccaggcgagt aaattcaaag agggtctttt tagtaggcgc ttcaacatca cggtgtttcc
     8521 atcgaacaaa gtgcgcatat gctcgcaaag caaaattaag ccaaccttaa gttcttaagg
     8581 ttggctttta tgtttgctgg attaagggga attaatcttt gcttattggt gcatctaagg
     8641 gatacgggtt tttatgtagc cggttgtaat tcagcgcata catggcggtg atgaccatca
     8701 gggtgacaaa tgaccacata acctctttcg ctccggaccc caccacggcc cagatgcagt
     8761 agaggaaggc aatggtagta actgccagat atgccgggcg tgctttacca aagtgaccgt
     8821 gtccgagcag cagtaacgcc gcacaggtgt aaagatatgg caccagtgta aagatgaccg
     8881 agacggaaga aaccagaccg aactctttgg tcgcgtttgg tgaaatgctg ctgagctgga
     8941 agatggtcat caaaataccg acgataatca gccccgccac tggcgtaccc gctttattta
     9001 cacgggcaaa aattggtggg aacagtccgt catcggcagc ggctttcgcc gtttgacccg
     9061 ccagcaacgt ccagccgccc agtgaaccta agcaacccgc agctgcgcag aaggaaacaa
     9121 tggccccggc ggtgtcaccc aacgccatcc gtgcggcatc accgaatggc gaagcagaaa
     9181 cgcgcagtgc ggcattaggg atcatcccca taatcgcggt ggtagaaagt acatagcaaa
     9241 cggcggcaat caatacccca ccaatggtgg cgatagggac attgcgtttc gggtttttca
     9301 ccacacctgc ggcaacggag gcactttcca caccgatgaa cgaccacagc gtaacgttaa
     9361 gggtactttg aattgcaccg aaggtgccca ggccgctgac gttccatgcc gccatatagg
     9421 tttcaccacg gaaccagaac cagccaaata cggcaatccc gacgatgggg atcagcgcca
     9481 gcacggtggc aactgcctgc acacgggtga tcattttcgg accgacaatg ttcagcagga
     9541 cgaagatcca cagcaccacg acgcaggtga tggttaatac caatggatct ttcagaatcg
     9601 ggaagaagta acttaaatat cctacgccaa tgaccaccat ggcgatattg ccgatccagc
     9661 aggccagcca gtagaggacg ttggtttgat aaccgagaaa cgggccaaag cagcggcggg
     9721 cgtaagcgta agaaccacca ggacttgggt cgaggaacga cattttggcg tataccatcg
     9781 agagccccag tgcaccgata atcgtcacca accatccata aatggcaatc ccgccagtag
     9841 aggccaggtt tgcaggtaac agaaaaacac ctgaccccat aatattcccc gacaccatca
     9901 gggtgacggg gattaagccc actttgtgag catcagcatc cgaagacata attaaactcc
     9961 tgcgaaggcg agcttcgtga caataaatta cgtcatatca tacgcctgca ttcgatagtt
    10021 gaattattat cgaccgggtt atcgctgatg ccgttattaa tatagctctt cgttaaggtt
    10081 aaaaatcagg cgacagtgcg ttctctgtgc tgactaacat aatgcagcgg cgtcataccg
    10141 tagaagtctt taaagacaga aataaagtac gacgtactgt tgtagccgca ggactgtgat
    10201 acctgagaga tatttttacc gtccatcatt aattcattta cggcataacg catgcggcag
    10261 gtggtgatta tttggctata actggtgttt tcgcttttca actttttctt taacagactt
    10321 gggctaagac ataaacaact ggctaccata ctaagattcc agtctttgtg aatatcgctt
    10381 tcaataattt gataaacgct gtcacttaca caattacgta acatatacat cattaatgaa
    10441 aggaattttt tactgtcgag aaaacgggat agcaccgtga ataataatgc gcgtgttcgt
    10501 tccttttctg actcagtttc tgcgggcatc atgctgtgtt gcgcggcaac gcgaaagacg
    10561 tctggcgtca ggcatggcag ggtgagtatg ggcgtagcgc tacgttgcca tactggtatt
    10621 tgcgagagat ctttattcag aaaccggagg taatctttga tgatgtcgtg actaatatga
    10681 gcaaccaaag tgttgttaag agaggagacg tcgataatat tattttcaca atttaataac
    10741 gccatatggt tagctttaag gctaataggt tctttcccat tcacccttat ccagacatct
    10801 ttttcagtca ataaaacaat acaaggttgg tcgctgcaaa tcctcataca tgtactcctg
    10861 agtgcaaata aaagaccagt tctcgctaaa gcaaagcgat accgggaaat ggttatataa
    10921 ttgtatgatg aatataaaca tgcataaaaa taacgagttt tactaatatt acaaattttt
    10981 atagtaattt ctcatgtaca tattttttta caggataaat aacaggcaaa aaaaagcgcg
    11041 gggtggggcc gcgcaaaaag tatgacattt ttgtttgcag caatttttat gaaaacgttg
    11101 ccatactcaa aaggcctaag gccaggcggc gtaatgttat ttaaacaatt acgccttcag
    11161 cggaatagtg gttacgcttt cacgcacata acgtggtaaa taccgtcaat aatttcagtc
    11221 ccttcagttt cgtgttcaaa tccagggaaa tggtggtccc aggattgcag cgagcgtaaa
    11281 taacttactt gcggactgtt tttatcgccg aagttttcac cagacagcag catcgggatt
    11341 cctggcggat acgggataac tgagtttgcc gcgatgcgtc ctggcagatt ttcaatggat
    11401 accagttcga cattgttgtc gacaatcgcg ttgtacgctt cacgcggggt gatttccgcc
    11461 atcggcaggc cggaataggc ttcgttcaac cgtgcgccag ggttgttttc tttcagccag
    11521 gcaaacatgg tgtcacccag atcgtgaatc cccatgttcg cgtaagtgtc aggatattgt
    11581 tcaacaagtt ccggcatcac ctgcgccagc ggtgtgttgg cgtcatagtg gcgtttgaag
    11641 gagcaaaggg tgttaaccag agtcccccat ttcccacggg ttacgcccat agagaacagg
    11701 aacataattt ggaagtcagt ggtgcgggta ggcacaatgc cgtggcgacc aagccaggca
    11761 gtgaccagcg ccgccggaac accggtttct tccagttcac catcttcacc cattcccgga
    11821 gcaaggatgc tgactttaat cgggtcgagc atactccagt tatccggaat atctttgaag
    11881 ccgtgccagc tttcgcccgg atgcattacc cagcagtcct gaacggtggt cagcagtttg
    11941 gttggtgcgt cagcaaagtc ataggttttg ccggtttgtg ggtcggtgac gacttctttg
    12001 ttccacggtt tgaagaacca gctaccgtca gcggtgaact ctttatacag ccgcgccatc
    12061 gcctgacgaa aatcaaccgc ttcgtcaatc acttcttgtg tcagtgacag gccgctgttg
    12121 ccgtccatca tcgacaccgc cacgtcgttg gatgcgcaga tggcatacag cggggaggtg
    12181 gtggcatgca tcatgtaggc ctggttgaag cgggagaagt taatcgcccc acgaccttca
    12241 cgtacatgaa tataagaagc ctgtgacagc gcattcagca gtttgtgggt ggagtgggtg
    12301 gcgaaaacgg taggaccgtt gtgatcgcca ggttcgccgc gcatggcata gtgatcggca
    12361 tagatcgggt tgaaacgtgc atagccgtac caggcttcgt caaagtgcag acgatcggag
    12421 gttttttcca gcagatcctg cgcttcttta gcgttataac acacgccgtc ataggtgcag
    12481 ttggtcacca cgcagtaaga cggtttttgc ccagctttgt ctttagtcag cgggctttca
    12541 ctgattttct tctgcaaggt ttcaggttgt atttcctgcg gatagattgg cccgataatg
    12601 ccgtagcggt tgcggcttgg caccatgtag actggtttcg cgcctgtcag catcaagcct
    12661 tgttcgatgg atttatggca attacggtca acgaccacga catcgttatc ggtcatgcaa
    12721 gcctgcatga tggtgcggtt agagccggaa gtaccgacga ctaccgacca ggagcgatcg
    12781 gcaccaaata cgcgtgcggc atatttttcg ctttcgccaa atgcgccagt atggtcaagc
    12841 aaagaaccga gggaagttcg ttcgatgccc atgtcggtgc gaaacagatt ttcaccatag
    12901 tagtcatggt agaaacgtcc ggcgggtgtt ttggtaaaac caacgccgcc ctggtggcct
    12961 ggcgctgccc aggaatattc atggatgtca ctatatttca tcagcgcgct gaacagtggc
    13021 ggcaacagct gctggcggta gcgggtcatc gcggcaacgg cgcgtccggc gataaagtcg
    13081 gcggtatctt ccagaatcca ggcgaattca tcgacaagct ccagcagatc ccggtccatt
    13141 gcggcgaggg ctttttcccg atcgcccaac aggaagaccg gcacgttttg ttggcgctca
    13201 tgaagcttac cgatcaattg tctgacgttt tgatgttcgt ccggatgttc catttgatag
    13261 ctgaacatca ggcagtcaat ggcttcgttt gaagagagaa tggcaaaacc atcatcaaag
    13321 gaggtggatt taatcacggt aacattttgc tggcttaaag catctgccag acgctcaacg
    13381 gcgttaccga cccaggtgtc ttgatggaga aactcgcttt caacaattaa tactttcatc
    13441 attgcttacc cggttatgaa ggaatgttcg ttgcgggcaa gtatcttccg gccttttcgg
    13501 gctgtaaagc gcgtgaaaaa caaacatttt tgaaatattt agttttataa acaatacgtt
    13561 atgtgattat tttaaattat tttcactgct tattatcgac ggctaaacta tttttttggc
    13621 tgatactgat atcgtcttta gccgggaaac gtctggcggc gctgttggct aagtttgcgg
    13681 tattgttgcg gcgacatgcc gacatatttg ccgaacgtgc tgtaaaaacg actacttgaa
    13741 cgaaagcctg ccgtcagggc aatatcgaga atacttttat cggtatcgct cagtaacgcg
    13801 cgaacgtggt tgatgcgcat cgcggtaatg tactgtttca tcgtcaattg catgacccgc
    13861 tggaatatcc ccattgcata gttggcgtta agtttgacgt gctcagccac atcgttgatg
    13921 gtcagcgcct gatcatagtt ttcggcaata aagcccagca tctggctaac ataaaattgc
    13981 gcatggcgcg agacgctgtt tttgtgtgtg cgcgaggttt tattgaccag aatcggttcc
    14041 cagccagaga ggctaaatcg cttgagcatc aggccaattt catcaatggc gagctggcga
    14101 atttgctcgt tcggactgtt taattcctgc tgccagcggc gcacttcaaa cgggctaagt
    14161 tgctgtgtgg ccagtgattt gatcaccatg ccgtgagtga cgtggttaat caggtcttta
    14221 tccagcggcc aggagagaaa cagatgcatc ggcagattaa aaatcgccat gctctgacag
    14281 gttccggtat ctgttagttg gtgcggtgta caggcccaga acagcgtgat atgaccctga
    14341 ttgatattca ctttttcatt gttgatcagg tattccacat cgccatcgaa aggcacattc
    14401 acttcgacct gaccatgcca gtggctggtg ggcatgatat gcggtgcgcg aaactcaatc
    14461 tccatccgct ggtattccga atacagcgac agcgggctgc gggtctgttt ttcgtcgctg
    14521 ctgcacataa acgtatctgt attcatggat ggctctcttt cctggaatat cagaattatg
    14581 gcaggagtga gggaggatga ctgcgagtgg gagcacggtt ttcaccctct tcccagagag
    14641 gcgaggggag cgtccgagca tcatgaggcc gaaaactctg cttttcaggt aacttattac
    14701 cataaactca gatttactgc tgcttcacgc aggatctgag tttatgggaa tgctcaacct
    14761 ggaagccgga ggttttctgc agattcgcct gccatgatga agttattcaa gcaagccagg
    14821 agatctgcat gatgactgca cccaaaatta catttatcgg cgctggttcg acgattttcg
    14881 ttaaaaatat tcttggtgat gtgttccatc gcgaggcgct gaaaacggcg catattgccc
    14941 tgatggacat tgatcctacc cgcctggaag agtcgcatat tgtggtgcgt aagcttatgg
    15001 attcagcagg ggccagcggc aaaatcacct gccacaccca acagaaagaa gccttacagg
    15061 atgccgattt tgtcgtggtg gcatttcaga ttggcggtta tgaaccttgc acggtgactg
    15121 atttcgaggt ctgtaagcgg catggtctgg aacaaaccat tgccgatacg ttggggccgg
    15181 gcggtattat gcgcgcgcta cgtaccattc cgcatctgtg gcaaatttgc gaggacatga
    15241 cggaagtctg ccccgatgcc accatgctca actatgttaa cccaatggcg atgaatacct
    15301 gggcgatgta tgcccgctat ccgcatatca aacaggtcgg gctgtgccat tcggtgcagg
    15361 gaacggcgga agagctggcg cgtgacctca atatcgaccc ggctacgctg cgttaccgtt
    15421 gcgcaggtat caaccatatg gcgttttacc tggagctgga gtgcaaaacc gccgacggca
    15481 gttacgtgaa tctctacccg gaactgctgg cggcttatga cgcagggcag gcaccgaagc
    15541 cgaatattca cggcaatact cgctgccaga atattgtgcg ctatgaaatg ttcaaaaagc
    15601 tgggctactt cgtcacggaa tcgtcagaac attttgctga gtacacaccg tggtttatta
    15661 agccaggtcg tgaggatttg attgagcgtt ataaagtacc gctggatgag tacccgaaac
    15721 gctgcgtcga gcagctggcg aactggcata aagagctgga ggagtataaa aacgcctccc
    15781 ggattgatat taaaccgtca cgggaatatg ccagcacaat catgaacgct atctggactg
    15841 gcgagccgag tgtgatttac ggcaacgtcc gtaacgatgg tttgattgat aacctgccac
    15901 aaggatgttg cgtggaagta gcctgtctgg ttgatgctaa tggcattcag ccgaccaaag
    15961 tcggtacgct accttcgcat ctggccgccc tgatgcaaac caacatcaac gtacagacgc
    16021 tgctgaccga agctattctt acggaaaatc gcgaccgtgt ttaccacgcc gcgatgatgg
    16081 acccgcatac tgccgccgtg ctgggcattg acgaaatata tgctcttgtt gacgacctga
    16141 ttgccgccca cggcgactgg ctgccaggct ggttgcaccg ttaaaacgcg actaaacgct
    16201 actgcgccgg gggaattatt ccggcgcaca cctctgacga taccaataac agaaggcggg
    16261 cgttggtaac agcgacccga taccctatga gcatttcaat gactacaaaa ctcagttatg
    16321 gatttggagc gttcgggaag gattttgcga tcggcattgt gtatatgtac ctcatgtatt
    16381 actacaccga tgtcgtcggg ctgtctgtgg gtttggtcgg tactttgttt ctggtggcga
    16441 ggatctggga tgctattaac gatccgatta tgggatggat tgtaaatgct acgcgatcgc
    16501 gatggggtaa gttcaaaccc tggatcctga tcggtacgtt ggcaaactct gtaatcttat
    16561 ttctcctctt tagtgcgcat ctgtttgaag gtactactca gattgtcttt gtttgcgtga
    16621 cctacatcct ctggggcatg acttacacca ttatggatat tcccttctgg tcgctggttc
    16681 caaccatcac gctcgataaa cgtgagcgtg aacaactggt tccttatccg cgtttttttg
    16741 ccagtctggc gggctttgtt acggcaggtg tgacgctacc atttgttaat tatgtcggcg
    16801 gtggcgatcg gggatttggc tttcagatgt tcactctggt actgatcgcc ttttttattg
    16861 tttcaaccat catcactctg cgcaatgtgc atgaagtctt ttcgtcagac aatcaaccgt
    16921 ctgctgaagg aagccatctg acacttaaag ccatcgttgc gcttatttat aaaaacgatc
    16981 agctttcatg cctcttgggt atggctcttg cttataatgt agccagcaat attattaccg
    17041 gctttgctat ctattatttc tcatatgtta ttggtgatgc ggatttgttc ccctattatc
    17101 tgtcgtatgc gggagctgct aacctggtga cgttagtatt cttcccacgc ttagttaaat
    17161 cattatcccg acgcatttta tgggccggag catctattct tccggtgtta agctgtggtg
    17221 ttctcctgtt aatggcatta atgagctatc acaacgtcgt cctcattgtg attgcgggta
    17281 ttttgctgaa tgtgggaacg gcgcttttct gggtattaca ggtcatcatg gtggcagata
    17341 ccgttgatta cggtgaatat aaactgcacg tacgctgtga aagtatcgct tactccgtgc
    17401 agactatggt ggtgaagggc ggttcagcct ttgcggcttt tttcattgcg gttgtgttag
    17461 ggatgattgg ctatgtaccg aatgttgaac agtctacgca agccctatta ggtatgcagt
    17521 ttattatgat tgctctacca actctgtttt tcatggtaac gctgattctc tacttccgtt
    17581 tctatcgcct caatggcgac acgctgcgca ggatccagat tcatctgctg gataaatatc
    17641 gcaaagtacc gcccgagcct gttcatgctg atattccggt cggtgcagtg agtgatgtga
    17701 aagcctgacg tggacaatag ctaacgaaaa tggcctgatg cgatatgttt atcaggccac
    17761 gtcttaatgt aatgctttgg atttgcaaat tttgtagacc ggataaggaa ttcacgccgc
    17821 atccggcatc aacaaagcgc acgttgttat ccggttatca agccaaagcg ccgtagctgg
    17881 cggcaatgaa agcgagcgag gaatatcacc gtcgttaatg cgccaagcag cgcgcagaac
    17941 atatcggatt gcgtgtccca ctggtcgccc tgggtgccga gaaaatcatc cgctccctga
    18001 cccattgcca gcgctgccca ccactcgatt aattcataca tcgcgcttat cgccagcgcc
    18061 acgcagcaga ccaggaacgc caccatttta cgtccgcgca cgtacatccc gcgaacgaga
    18121 atttctcgtg ccaccagtgc aggcaccagc ccctggaaaa agtgccccag cttgtcatac
    18181 ggattacgac tcaaccccag ccattcctgc acctcaaaac caacggggac tttcgcgtag
    18241 gtgtattgcc cgccgaccat caggatgatg gcgtgaaaga aaatgagcgt atagagcagc
    18301 ggcgttaacg gataacgtct ggcggtggca agcagtagcg gcacgacaat aatcaccggt
    18361 gtcacttcca tcagccaggt gagtttgtca tgggccgaaa tgccggtata aatcaggatt
    18421 aatgttagcg ccagtgcgcc ggtgttaaga attaacggct tgagtgtgcg ggtcatggtg
    18481 agttcgcaag tcagggaaaa tactgactat tcaccagcaa gcatgaaatt acaatcgcaa
    18541 aagaggtggc ccagggggat cacctggcag catgctgcca ggcgctgggc cgaagaggtt
    18601 acttagtgca gttcgcgcac tgtttgttga cgatttgctg gaagaagtcg ttacctttgt
    18661 catcgaccag gataaacgcc gggaaatctt ctacttcgat tttccagata gcttccatac
    18721 ccagctccgg ataagcgacg cattccagat gcttgatgct ctgctgcgcc agtaccgccg
    18781 ccggaccgcc gatgctaccg aggtagaagc cgccgtgttt atgacacgcg tcggtaacct
    18841 gctgactgcg gttacctttc gccagcatga tcatgctgcc gccgtgggat tgcagcagat
    18901 ccacgtagga gtccatacgg cctgcggtgg ttgggccaag tgaacctgat ggataaccgg
    18961 caggggtttt cgccggaccc gcgtagtaga tcgggtgatc tttgatgtac tgcggaagtt
    19021 ctttaccggc gtcaatcagc tctttcagct tggcgtgtgc aatatctcgg cccacgataa
    19081 tggtgccggt gagcgacaaa cgagtggata ccgggtattg cgaaagctgg gcgaggatct
    19141 ctttcatcgg gcggttaagg tcaactttca ccgcttcgcc ttcaccggcc tggcgcagtt
    19201 cttgtggaat gtactggcct gggttgtgtt ccagtttttc gatccagata ccttcgcggt
    19261 tgattttcgc tttaatgtta cggtcagcgg agcaggagac gcccatgccg accgggcagg
    19321 atgcgccgtg acgtggcaga cggataacgc gaatgtcgtg cgcgaagtat ttaccgccaa
    19381 actgcgcgcc aagaccgagt ttctgggcct cttccagcag ttcctgttcc agctggacat
    19441 cgcggaacgc ctgaccatgt tcgttccctt ccgtcggcag ttcatcgtaa tagtgagcgc
    19501 ttgctaactt gacggttttc aggttggttt ccgcagacgt accgccaatc acaaacgcga
    19561 tatggtacgg cgggcaggct gcagtaccga gggtacgcat tttctcgacg aggaagtttt
    19621 tcagtttgcc gggagtcagc agggctttgg tttcctggta gagatacgtt ttgttggcag
    19681 agccgccgcc tttcgcaacg caaaggaatt tgtactcatc gccatctacc gcgtacaggt
    19741 cgatttgcgc aggcaggtta gtgccggtgt tgacctcttt gtacatgtcc agcgccgcgt
    19801 tttgcgagta gcgcaggtta tcttcgatat aggtgttata gacgcctttc gacagcgctt
    19861 cttcatcacc gccaccggtc cacacgcgct ggcctttttt accgacgatg atcgcggtgc
    19921 cggtatcctg gcaggtcggc agcacgcctt tggcggcgat ttcggagttt cttaagaatt
    19981 gcagcgccac gtacttgtcg ttttcgctgg cttctggatc gtgaagaata gccgcaacct
    20041 gtttctggtg tgccggacgg agcatgaaag aagcgtcgtg aaaggcttgc tgcgccagta
    20101 gggtcagggc ttctggttcc actttcagga tggtttcgcc gtcgaagtcg gcaacgctaa
    20161 cgtaatcgga agtgagtaga tagtattcgg tattgtcttt ccccatcggg aaaggtgcct
    20221 ggtagataaa gggtttgttt gacatagctt ccagcctgta actctgtatt tgttattcga
    20281 aaatggcgtg ccgcgtagtc acgggcggca cgcaaagtgc atttataaga acccgtacat
    20341 cgcggcgaag atccagccga agacgcacga tacgctcaca ccaatcaacc ccggcagaat
    20401 aaaactgtgg ttgatgacga agcgaccgat gtgggtggtg ccggaacggt caaactgaat
    20461 cgctgccaga tcgctcggat aagtcggcag gatgtaataa ccgtagcaag ccggtgctga
    20521 agccacgatg tatgccggat caacgccgat cgccagcgcg accggaacaa tcgccgccag
    20581 cgccgcagcc tgagagttta caaacttgga aaccagcagc agaacaatgg catacgccca
    20641 cggatactct ttcaccattt cacccagtac gccttgaatt tcagacatat gcgcaccgaa
    20701 catggtttct gccatccatg cgataccgta caccgccacg atggcgatca taccggaacg
    20761 gaagacttcg ttttttgaga tagacgcggg attggttttg gtcaggataa taatcagcgc
    20821 cccggtcagc agcataaaca tctgaataac cagtaccatc gacagcggtt tgccgccgaa
    20881 ggatggacgc aggtccgaat cagcaccaag aagtgcgact acagcgattg caccgaggaa
    20941 aatccacatt gccagccagt tgcttttcgg cagtttttta tcgagcagcg tcgcggtatc
    21001 accgtaaaca tactcacggt tttccggtac ggagatgaat ttctggaact cttcgtcttt
    21061 atccagatct ttaccgcgga accagctgaa gataccgatc gccaggatac cgattaacgt
    21121 cgatggaatg gtgattgcca gcaggtcgag gaactcaaga tggcgaccat caaaggtgac
    21181 attacccagc attgcaacca gagacacgac cgcaaccgac accggactgg cgataatccc
    21241 catctgtgca ccgatagaac ttgccgccat cggacgttcc ggacggatgt tgttcttaat
    21301 ggcgacgtcg tagatgatcg gcagaatggt gtaaaccaca tgacccgtac cgcaaagaat
    21361 ggtcagtgta caggtcacaa acggcgcgac aattgagaca tatttcgggt tgcggcgcag
    21421 caacttctcg gcaatttgca gcatgacatc aagaccgccc gaagcttgca aggtcgccga
    21481 tgccgccacc accgcaatga taaccagcat gacatcaact ggtggtttac ctggctgaag
    21541 gtggaagacg aagaccagaa tgaccagacc gataccgcct aataaaccca gcgcgatacc
    21601 accctttctg gcaccataaa acagacatat cagtattatg ataagttgga tagtaaataa
    21661 catgtgtgaa ccctcgcgat aatcctattt aaatttttgc tgaatagatc acagtcacgt
    21721 tctgttttgt atgaactgtt ttcagagcct gactaaatat ccgtttggtc gttaccggct
    21781 ttagcaaata cctcacagtg aatattggct gattaaagca gcggcaaatt tatgcactgt
    21841 gataagcggc tttttcagag agaaagactc tctggcgatg ggtttatagt ttgtactgcg
    21901 cagatgttaa tgttttgttt gctgttatct tattgatatt tatcggtcta ttttttgttc
    21961 tcagggcaga tgtcattagg tttatagatt aatctgatct acccatttgt gggtaaaaat
    22021 acacataatg cgggtgacat aatagttaat taacttttgt tagcgttttg aaattaaaaa
    22081 caccgttcac ctgaagagat attaattttt agcgatgatg gagggataat tatatttgat
    22141 ctggcacaag ttttactgat gaaggatgta acttgtgcca ggggtatttt gcattacggt
    22201 aataattatt acttacagat aaccagcgat ccgttattgg caatattgtt tcagcagtga
    22261 gtagtgttct gcctgaatac ggtaacggta aaccggacgc cccgtgacgc cataatggat
    22321 actggtgaac aagatgtggc agttgaccag ccagatgagg tattttcggc aggaaacacg
    22381 cgagatgtta acctcgttag ccagctcgtc ggttgaaaac tcatagtcct gatgcgcgtc
    22441 aatccactgg cacagcgtgc gtaacgtctg cggcgttaag ccttttggca agcgacgagg
    22501 atcctgttcg ttggagctgc tgccgtggat tagctgatca agctcggcct ggtcataata
    22561 ctgatgtttt tccagcgcca ttttcttttg ccgccagccg gtgagcgcct cttcaaagcg
    22621 ggaagcctgg aagggtttga tcaggtaatc cacgacaccg taatgcagcg aatctttaat
    22681 ggttgccgca tcggctgcgg aggagatgac aatcacatca cttttgcaac gcgcgttatg
    22741 caggacaggc agtaaatcga gcccgttctc tttttgcata tagatatcga gcaatatcag
    22801 gtcgataggc gtatcgctat tgaagataat ctctttggct ttctccagcg tcgaggctgt
    22861 tccacagcat tgaaagcctg ggatttgtgc tacgtatcgg cgattcagct ccgcgaccat
    22921 tgcgtcgtca tcgataatta atacattgat catctgttcg acctctcccc gtcccagggt
    22981 atctggacaa aaaattgtgt gaaaatcccg ggttccgatt ccacggcgat gctgccgccg
    23041 agattttcta cctgttgttt gacaagtgct aaaccgacgc ctcgctcgct tccttttgtc
    23101 gagacacctt tgtcaaaaat gtgatcgatt ttgtcgggtg cgatccccgg tccatcatcg
    23161 ttaacttcgc agtgcagcca gccgtgacgg tagtgcaatg ttacgctaat ttcgccaccg
    23221 ggttccggcc ctaatgcttc cagcgcgttt tctatcagat ttcccaatgt ggtaatcagc
    23281 gtcgcgacct ggtcctcact gccgctgtct ggcagcaggc tttcactgtt taaaatcagc
    23341 gtatggccta aatcggtcgc gcggttaatc ttgctgatta aaaaaccagc gataaccgga
    23401 gatttgatct tacccagcag agagccaatc tcttcctgat agttattggc tgttttgaga
    23461 atgtaatctt ccaactgctt ataactcttc agatgcaata atccgagaat cacatgcaat
    23521 ttattcataa attcgtggga tcgttcacga agtgcatcag catagttgac cagaccgtcg
    23581 agtcgctgca tcagtttacg aacttcagtt ttgtccctga aggttgaaat ggcaccgatg
    23641 ataacgccat tactgcgcac cggaacggtg ttgatcagta atagccggtc tttaatcgta
    23701 atctcttcgt cgcggcgcgg cgtaccgtcg cgtaacactt ccgagacatc taccacctgt
    23761 gaccatgagt ggcttagcgt cgacagtttc tcatcgtcct gcgacttacg gtaattcagc
    23821 aattcttgtg cggcatcgtt gatcagcgtg acctcgccgc gatcgtccac ggcaacgacg
    23881 ccttctttga tagactgcaa catggcctgg cgttgctcaa acagcgtgga gatttcgtag
    23941 ggttccaggc cgaaaaggat ttttttcagt accttaacca gaatgcaggt gccaatcagt
    24001 ccgaccagca tgccaaataa taccgaccag ataatgctcc agcgactgtc attgatttgt
    24061 tgggtcacgc ggcttaactc aaggccgatc gccaccacgc caatttgttt atgattttca
    24121 tcgtagatgg gggtaaatac gcgtaaagcc tgtgccagaa aaccgcgatt gatagcgaca
    24181 ttttctttgc cattcagcgc tttaaggatg tcatcacctt taaatggctg accaatacgc
    24241 tgggcttcag gatgcgagta gcgaagactt tgcatatcgg taacgacaat aaacagcaga
    24301 tcgttgcgtt tgcgcacggc ttccgcgatg gcctggattc cgctctcctg cggttttttc
    24361 tgcaagccct gacggatttc cggcgagtcg gcgagggtac gcgccattgc cagtgccttg
    24421 ttggccagcc cgtctcgcgt catatcactg atttgcgaga agtaaatcag atgcaccacc
    24481 aatagcaccg agaacagtac cgcgctgacc attaagatca ctgtggtact caatttcatc
    24541 ggacgtttgc gtaacatgcg gtagggcaat gaatgtctca tcagcttcct tgtgtgacaa
    24601 atttcttaag cattatctct gatgaggcgg gtaattcaaa gggagtaaga atgattggct
    24661 atatagggga agagactctg gcaacggaaa ctgccagtgc tgtatgaaga ttccagggct
    24721 atgcttatag cgataatcat actgatgaga gagggaaagt catggatcag gcgctactgg
    24781 acgagggtta tcgctgttat accggcgaaa agatcgatgt ctatttcaac actgcgatat
    24841 gtcagcattc tggcaattgc gtacgtggca acggcaagtt atttaatctc aaacgaaagc
    24901 cgtggatcat gccggatgaa gtcgacgtcg tcactgtggt taaagtgatt gatacgtgcc
    24961 cgagcggcgc gctgaaatac cgtcataaat aagcgagggt aaaatggaaa tacgcgaagg
    25021 ccacaataaa ttttacatta atgacgaaca aggcaagcaa atcgctgaaa ttgtctttgt
    25081 gccgaccgga gagaatttag cgattatcga acataccgat gtcgatgaaa gcctgaaagg
    25141 gcaagggatt ggtaaacagc tggttgcgaa agtcgtggaa aaaatgcgtc gggaaaaacg
    25201 aaaaattatc ccattatgtc catttgcgaa acacgaattt gataaaacgc gggagtatga
    25261 tgatattcgc agttgatggg agagtacaga gtcacgatat ttttcttgct ctccgcggag
    25321 tgatgcagga gagcatctga agggtagggg gatgcacaaa gaatgggcag agagcgcgtt
    25381 tttttgtccc aagtcatccc ctttactgag caaaaaaaag aatatctcct atatgagaat
    25441 catcaatcgg ggttaataat ttttgcgtcc ccagagcatt caatattgat aggagtcata
    25501 ttatggaagg taaaaacaag ttcaatactt atgttgtttc ttttgattat ccatcatctt
    25561 attcctcagt gttcttaagg ttaagatcat tgatgtatga tatgaatttc tcttctatcg
    25621 tggctgatga atatgggata ccacgacaat tgaatgaaaa ttctttcgca ataacgacat
    25681 cgttagccgc aagtgaaatc gaagatttaa tcaggcttaa atgcttagac ttaccggata
    25741 ttgattttga cctcaacatt atgacagttg atgactattt ccgtcagttt tacaagtagc
    25801 caggaaggat aagaagaaca taaaaaatgg cactattctc taaaatatta attttttatg
    25861 tgattggtgt gaacatatcc tttgtcatta tctggtttat ctcacatgag aaaacacata
    25921 ttcgtttact tagtgcattc ctggtcggaa taacctggcc aatgagtctg cctgtggcat
    25981 tacttttttc tctcttttag gagctgtagt tgagtggacc cgctggttat cgtgaaacga
    26041 agcgtctctc ctcaaaactg gatttgcgga acgacgaacg aagcagtcgc ctgactgctt
    26101 cgttcattaa agtgaaattt atttctgtgg gcgcatcgcc gggaagagaa taacgtcgcg
    26161 aatagtatga ctgtttgtaa acagcataat cattcggtcg ataccaatac ccagaccagc
    26221 ggttggcggc agaccatatt ccagcgcagt cacgtaatct tcgtcataga acatggcttc
    26281 gtcgtcacct gcagctttag cgttaacctg ttcctggaaa cgttcagcct gatcttctgc
    26341 gtcgtttaat tcgctaaaac cattaccgat ttcacgacca ccgatgaaga attcaaaacg
    26401 gtcggtgatt tccgggttaa catcattacg gcgcgccagc ggtgacactt ctgccggata
    26461 ttccgtaata aaggttggct gaatcagatg tgcttctgcc acttcatcaa agatctccgt
    26521 gacaatacgt cccagccccc agcttttctc taccgtaatt ccgatagatt cagctaatgc
    26581 tttagcagca tcaaaattat ccaggtcggc catatcggtt tctggacgat attttttgat
    26641 tgcttcgcgc atggtgagtt tttcaaacgg tttgccgaaa tcaaacacat gctcgccata
    26701 agtgacttta gtggtaccca gaacctcttg tgccagggtg cggaacaggg actctgtcag
    26761 ttcaatcaaa tcgtggtaat ccgcatacgc catgtagagt tccatcattg tgaactcagg
    26821 attatggcga acagaaatac cttcattacg gaagttacgg ttgatttcga atacccgttc
    26881 aaaaccgcct acaaccagac gtttcagata cagctccggc gcgatacgca ggtacatatc
    26941 taaatccaga gcgttatgat gggtaataaa cgggcgagca gatgccccac ctggaattac
    27001 ctgcatcatc ggggtttcta cttccataaa gccgcgcgcg accatgaact ggcggatagc
    27061 ggccagaatt tttgaacgga caacaaacgt ttgacgggat ttatcgttag cgatgaggtc
    27121 cagataacgt tgacgataac ggacttcctg atcctgcaga ccatggaatt tatctggtaa
    27181 aggacgtagt gcttttgtca gcaggcgcaa ctcagtacag tgaatggaaa gctcaccggt
    27241 ttgcgtctta aacagcgtac cgcgggcacc gataatgtca cccagatccc attttttaaa
    27301 ctgatcgtta taaacacctt ctggcaggct atctcttgca acgtacagtt gaatacggcc
    27361 accgacatcc tgcaacgtta caaaggaggc tttccccatg atacgacggg tcatcattcg
    27421 gccagcaacc gagacttcaa tgtttaagga ttccagttcc tggttatcct tcgcatcaaa
    27481 ctcttcgtgc agctggtcag aggtatggtc gcggcgaaaa tcattgggaa acgccacacc
    27541 ttgctgacgt agtgctgcca gtttttcgcg gcgatttctc agttcatcgt taaaatcaat
    27601 agcctcattg gctccccgtg tttcttgttc agacattttg gttcctctaa atccagcttt
    27661 caatttagcg tagataaaga gacagatcgg tacactgtat tatctgcctc gactattaat
    27721 aactcagttt atcttatcga aactaacgct tacctcaagt tgatatcact aataaataac
    27781 catcaaaatg gtttaaatga cttattaatc tcgatttact agtgtgatta actgaaagtt
    27841 aatttattca taaccaattg tttttacgac agttaatcgt tgctctcctg tatcatattc
    27901 gtcggcgtgc taaacagaaa acgggtagca aaggccaata cgacgatgat cgcgacacaa
    27961 gccaacgtcc actctcccat ctgagaaaag aatcgctggt aagctgcaat tgccataccg
    28021 ctaatttgcg actctgtcgt ctgctgtgcc acgacgcctg ccagccagtt ggcgaccgcg
    28081 cctgttgcca gcatataaat accggttaat acgccagaca ttttcagacg cgtgatttgc
    28141 gcaatcgcca ccgggtcaat aaagagttcg gcaaagccca ttagcgccag cccggatatc
    28201 atcacggcca ttgacgcttg accgccagcc gctgcatgtc gggcatcaaa tgccaacaac
    28261 ataaagccac aagccatcag cagtaagcca aaggcaaact tcagccagac gcgcaatgtt
    28321 gagttgccgc ggctttctgg cgacgccagc caggccagta caaccccagc gagcatcact
    28381 gcaattgcat tcaccgactg gaatagtgct gtaggtactt caatattgaa tgcctgacga
    28441 ttcacaaagc gatcgataaa caagctgatg gtactgccgc cctgttgtgc cagtacccag
    28501 aacaacgtcc cgacaaacat caacaataca atttgccaaa gagcacggcg gtgttcgggg
    28561 aatttgatca tcatgcgggc aatgatttgt gcggcaatga ggcaaacgat cgccagcaaa
    28621 tatccggacc agtcgttctc cagcagcagg gtaaaaaata ctggggctaa acagagcatc
    28681 accactaacc agctccatac cggtaaggca aatttgacgc tagtgagcgc ttttttatcc
    28741 atactacgcg tggactggaa atgacgatgc ccgcttaaga aaatcaacaa accgataaac
    28801 atgccgccac ccgcaagggc aaagccaaca tgccatccat accactgagc agccaggccg
    28861 caggcgattg gggcggcgat agaaccgata ttgcccgcag catacagcag cgaaaaaccg
    28921 ccatcacgtc gatgatcgtt ctcgtcgtaa agctcgccaa gcaaacagct gatgtttgat
    28981 ttgaataaac cgtagccaca aataatgatt gccagcgcca gatacaggct aaaggttgaa
    29041 tttgtatcaa tgcccagcac cacatggcca agggtcatta acagcgcgcc ggcaatcact
    29101 gcagtgcggt tgccgagcag gcggtcggca agccagccgc cgagaatagg ggtaacgtaa
    29161 accagagaag catatgcgct gaacaggctg atggcatggt tatcatcaaa accaagctga
    29221 tgggtgagat agagaatgag taaggcacgc atgccgtaaa aactgaagta ctcccagatt
    29281 tggatcgcca cgatatagta tatcgcgcgc ggctgtgagg gtgttttcat gtgttctcct
    29341 tatgagcaaa aaagggaagt ggcaagccac ttcccttgta cgagctaatt attttttgct
    29401 ttcttctttc aataccttaa cggtatagcg gccatcagcc tgacggtatg caccgtgaat
    29461 atcggtttca aagcccggat agtgagcgcc gatttcacac agcatctgca ggaactccag
    29521 aaccggacgg ctttcttcgg tgatcatttc acccggcatt accagaggaa ctcccggcgg
    29581 atatggaagg atcatattgg cgttaatacg acctaccatt tcgtcgaggt aaacttcttc
    29641 ggtcataccg tgcagctctt tctggaacgc agcatacgga gtcattacca tcgtcggcag
    29701 cacttcaaat gcgcgataca tcagatccgg cagattgtgg tgaacaatca gtttgtggat
    29761 attctgagcc agttcctgaa tacgcatgtt ttcatagaat tcaggatctt cacgatacag
    29821 agacggcagc atgtttttca cacgcaggtt caggtcgaac gcacgtttga agtcagtcag
    29881 agcacgcagc aggctcagtg ctttggtctt atcgataccg atgctgaaca ggaacagcag
    29941 gttatacgga ccggttttct caacaacgat gccatgttcg tcgaggtatt tcgccacgat
    30001 gctggccgga ataccaaagt cgctcatggt gccgtctttt tccatccccg gagtcagcag
    30061 ggtgactttg atcgggtcaa gatacatgtg ctcgttatcg atgtttttga agccgtgcca
    30121 ggtgctgtca gaacgcagcg gccagcattc agtcgtatcg atatgatccg gctgccatac
    30181 atcaaagaac cagccatcag attccgttct cagacgtttg atctctttac ggaatttgat
    30241 cgcacgttca atggaaccgt tgatcagacg tttacctgca ttgcctttca tcatcgccgc
    30301 agcggtttca gtggacgcca cgataccgta gtgcggagaa gtggtggtgt gcatcatgta
    30361 ggcttcgtta aaggtttctt cgtttacgtc acctttaacg tggatcatgg aagcctgaga
    30421 gaacgccgcc agcagtttgt gagtggactg ggtttcgtaa atcactttcc cttctacacg
    30481 gccaccgctc ataccgcatt taccttcgta aatcggtgag aagttggtgt aaggcaccca
    30541 cgcggagtca aagtggatgg atttcacatc cagtgttttc ttgatgaagt cggtgttgta
    30601 cagcagacca tcataggtag agttggtaat tacagcatgt accggccagg ttgcgtttgg
    30661 tgtttctttc acgcgcttag caatggtagc gtgctggaat tcactctgtg ggataccacc
    30721 aagaataccg taagcgttac gggtcgggcg gaaatagatt ggcgtaacat cgctcatcat
    30781 catcaggtgg gtcagcgatt tgtggcagtt acggtcaatc agaatggtgc tgcctgccgg
    30841 agcagagtac atgccaacaa ttttgttcgc agtggaagta ccgttggtca ccatgtagct
    30901 gcggtctgcg ttaaagacgc gagcgatata ctgttctgct tctttgtgtg gaccactgtg
    30961 atccagcaga gaacccagtt cagatactga aatggaaata tcagatttca tggtattcgg
    31021 accaaagaaa tcatagaaca ggctacctac cgggcttttc tggaatgcag taccgcccat
    31081 gtgaccagga gtacagaaag tatatttacc ttcacgaaca tatttaaaca gtgctttagt
    31141 cagcggaggc agaatagtgt tgatatattc gtcagtggtc tgcttgatct tattagcaat
    31201 atcttcagca gcacccagcg catattcaaa gaagctaatc tgtaaacgca ggtcattcag
    31261 gcttacatcg agagtggaat acgtattagc gaacgcgtac aacggcaggt tctcgttcat
    31321 tttgctaatt tcttcgcaca gctcgagatt atatttatcc cagtcaaaaa taacgccgca
    31381 cagacgcgca ttgttttcga tcagttttaa taagtcgtca cggtcgttcg ggtaaacaat
    31441 ctggaagttc agacgttcaa gcgcgcgatg aagttcacgg atgggttctt ctttaaaata
    31501 aacccccatg tgattcaata ttgcaataac gttcatagtc atatctccag gtaaaaaagg
    31561 cccctcccaa cacatgggac aaaatgaaag gaggagcctc ggaaaatact tttaattaat
    31621 gtgcgttaga cgcggtgtgg ttatccattg agtggctctg gcgctcgtgc attttgcgag
    31681 cgtagaacat caggataatc aggctgacga tgaaggtacc tgccagctcg aaggagcttg
    31741 cgcccatcag cgcgatgaag cagaacacac aacccagtac agagcagatc aggctgacaa
    31801 agttgcggat gttaacgcct tcaaaacgaa tcaggtcaac gcaagagtag aagtacggca
    31861 gcatagtcag cagtactgcg ataccggtca gttcaccgaa caggtcagat gctttaccac
    31921 cggcagagtt catcagagtg atgaggatca tcagggcagt cattttcact gcagccagca
    31981 gcagaccttt tttcggaata ccgttgctgt cgacttcacc ataaactttc gggaagttac
    32041 cgtcgttagc ggcacgtaca cctgcctggc ctaccaacat catccaggag cccagagaag
    32101 tcaggcacgc aaaggcggtg aatgcagaaa ccagcggcgc agcccagtta ccgaggatgg
    32161 ttgaagcact gattgcaaac ggagcaccgg aagctgccat tacagaagat ggatacatac
    32221 cggaaagcac ctgagtcgca gcgatgtaaa caatacctgc taaaccagta cccagcatgg
    32281 ttgccagcgg aacggtacgt ttcgggtttt taaccatacc agtacttact gctgcggatt
    32341 caacacccac gaaggcccac aggcagagca gaatactttt aatgatcgca tgaccatcag
    32401 tggtatccgc agtattccag ttagctgcat aagttgccgc atcaaaccaa tgccagccaa
    32461 caatagcagt catcaccaca ggaataagaa ccagcaccag accaatagtg gttaaacggc
    32521 ttacccaggt accgccgagc atatttacaa aggtaaatac ccagacgata gcaatacagg
    32581 cgatacccgc cggaacagga tcatttaata ctgggaagaa ggtggaaaga taagataccg
    32641 cggtaatacc aatcgccagg ttaccaatcc agttagcatg gtaataaaga acacctgtct
    32701 gaaaaccaaa tgcaggggaa atttctccgg cataagcaat tgggccacct tgttgcgggt
    32761 tttttgttgc cagtcgggca tatacatacg ccagcgacat tgcaccaata atagagataa
    32821 tccaacccca gatagcaata ccaccgatac ttgctaggtt cgcaggtaat aatgcaatac
    32881 cgctccccat catattaccg gcaacaacac cggtacaggc aaatagcccg atcttcttgg
    32941 cagaactcat gctcttctcc taatttcatt tttgaatttg gagtccgggt catgatgtat
    33001 aactatttcc tgaccagacc aaactggcgc taagaatact cacgaaaaaa ggattaatcc
    33061 taaagattag gtgaaataac acaaaagttt ctgtaagtga gaacttgagg ttttttatta
    33121 acacatcagg atcgcaagtt gatatcatga aaagataaac attcaatgtt tacaatggat
    33181 tgcgggatat tctctggtta aatttatgta ataaaaatta tgcggcaaat aaattgccgc
    33241 aacatattat accaacagaa acatacaaaa actcaacaac aaatattccc gagcataaat
    33301 caacccggga gttacttatt ctgaagcgag aaatttgtcg agataaggta caacataagg
    33361 aacagaagtc tggaatatac cattttcaat ccagtaaagg gtgtttgccc ctgggcgtaa
    33421 attaaaggcg gtgagatatg catcagccgc ttcccggttc atccccttca tttcataaac
    33481 cttgccaagc aacacataat ttagccagga catttcaaga tcaatgccag tatttatcgc
    33541 ctggtaagac tcatctgttt taccttttac cagagcactg accgctttta tttgatatat
    33601 aatggacagg ttattcaatt ccggcagtgt aacaatgttg tctatttctg tgttcagtgc
    33661 tgctaattgt ttttcatcta aagggtgttg agaatggcgc acgatatcaa ctaatgcttt
    33721 ttctgctctc gcgtaggtaa attctgggga tgattgaaca atctcaccta ataattcact
    33781 ggcacggttc aatgatttat catcgccatg cagtaaataa tcatgtgcct gataaaaatt
    33841 agttaataac gcaccacgat gaggcaatat ttgctggagc gtctcctgca ttcgttgtgg
    33901 ccacggttgg tttaacgctt ttgataaact ctccagtaaa tcattttgaa tcgccagctg
    33961 attaccgtta gtgatgacat aacgtttatc cagcatggtt gaaccatctg cattgtctac
    34021 caattttatc gacataaagc attgttgagc acggtattgg cgctgattaa caaacgcaat
    34081 agataatgtt ttaccggaac tgctcggttc atcaatgttg tagttgattt tgtcatgcac
    34141 cataaaggtg gagaaggtgt taagtgatgt cgccaccaaa tcacccacgc ctatcgcgta
    34201 agagagctga tacggggaac tccagctgtt acaactttta tttaccatat taatgtcaat
    34261 atcgcgtgga ttgagcaaaa tacgcgattt gctcatagga agacgtgtat caagagttga
    34321 aaacgctacc agtgctacac agatacctaa cgacaacagg aaaaaaaacc atacccaaaa
    34381 ggtagtgaat cgtttgcttt taactgggga ttgttcaggt ggcgttgcgg tgttttgaat
    34441 gttaagactg tgggagggag aatctgtggc aggaaccgcc tctggtatag ggggaggcga
    34501 agatagcatt atttcctctc cctcttcttc gctgtaccag ataaccggca ccattaattt
    34561 atagccgcgc tttggtacag tagcgatata gacaggacta tcttcatcat tatcttttaa
    34621 tgacttacgt agttctgaga tactctgcgt cacaacatga ttggtgacaa tacttctctt
    34681 ccagacatta tcgataagtt catccctgct aagtacttcg ccactgtgtt gagcaaagaa
    34741 aaccagaaga tcgattaatc tcggctcaag ggtaagttga cgcccattgc ggctaatttg
    34801 gtttatggac ggagtaacaa gccattcgcc aacgcgaact acaggttgtt gcataataga
    34861 aactcattcg aaaagggaat gatgcaatga taattgccac aacctatttt taccatctat
    34921 agatgggttt atttacatat tattggtgaa tgcaagacgt tatttttacc agtcataaac
    34981 ttcttgatta catagtatta cgaaaggatt ttactgagaa ccagaagtaa tattccttac
    35041 catcaaaatt catcatcttt gccaaagaaa aatgttcaga aaataatcca tggaaaattg
    35101 tccggagcgc ttactatttt aatggattgt tagtctttgc atgagcaagc ggactgatac
    35161 atttctcttt gttctcattc agaaaatctc atcagtcggc gttcagaccg gtgttgtgct
    35221 ttatggatgc gttacgctcc tgatgacgtc atttgacgtt caacaacatc acggggccgc
    35281 acgacatttc acgtcagtta gtgctatcgc tcaggaacaa attttcccga attgggatat
    35341 gcccgcaaat tgctggtgat gtggggagaa tctggttgag ttcgggagaa ttgatttgga
    35401 ggcagaacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
    35461 tatatgaatt tggtgcccgg actcggaatc gaaccaagga cacggggatt ttcaatcccc
    35521 tgctctaccg actgagctat ccgggcaacg gggcgcatta aaccgtaatc tgcacatctc
    35581 gtcaacctaa tttcagtaaa agcgattcaa ctgcttaaga ttgcggcaaa tcgcttcttt
    35641 tctgtgtttc tgtcaggtca atgcaccacc ctggcggcag cgggcgaagc gcaggatatc
    35701 ttccgccagc cgatgtgcag tgtcgacatc cgcctggcta cgattcacca gcattcggct
    35761 taaacagcct tccagcacca gttccatctg ctttgctacc atcgccggat cgtcaacttc
    35821 cagtgtggtt aacagttcgt gggtgaaatc gtaggccgcg cttttttgct gatcggccag
    35881 ttgatgaata gggtggccag gatcgggata aaacgtacag gcagcgataa atagacagcc
    35941 cggatagcgg ttgtttttaa cgcactccga taacgcctga taacgtgcca gcagcttttg
    36001 ttcggcggtt tgcgtttcgt ccagcatcag ctgacgacgc cagacatcta tctgttggct
    36061 aagataacgc agcgcatcgt agaggattgc ctctttgtct ggccagaagc ggcgtagctc
    36121 gtccagtgga taatccacac gttcagcaac catctccagc gtggtgttgg caatcccttg
    36181 taattctaat aatttcaggg cttctcccag tacatcttca cgttgcacgc tattttcctc
    36241 cgtctttccc actgcaagtg tcgttcacgg ttggcgatcg cgcaaatgtg cgctgaaggt
    36301 ttcagcatcc ataaagcccg tgacgcgtgc ttgtggatgc tcctggcctt gtccgtcaaa
    36361 aaagagaatt gtcggtaggc caaggacatt aagatgcttt aacagcgcca catcttgtgc
    36421 gtcgttggcc gtgacgttgg cctgaagtaa gaccgtgtct gctaacgctt tttgcacctg
    36481 cgggtcgctg aaggtgtatt tctcaaactc tttacaggcg acgcaccagt cggcataaag
    36541 atctaacatc accggtttgc ctttggcttc aacgagcgcc tgatttaact catctaccgt
    36601 tttgatttgt gtaaagttga gatgcgtctg agtttgcgcg gtatgcgtcg caccaaatgc
    36661 ccaatcctga agtgggcgca cgctaaccaa tgccgctgcc agcaggatta tttgcaccac
    36721 gcgcatccag ccgcgtttag cctgtaggct ggtgataaag gcccagccaa agaatgcgac
    36781 acccagcgcc gaccacaagc gtaatcccca tacatcacca atcactcgct ccagcaggaa
    36841 gaccggcagt gcgaggatca caaaaccaaa cgcggttttg acttgttcca tccacgggcc
    36901 gcttttcggc agcaagcggt taccaaagac ggtaattagc atcagcggca ggcccatgcc
    36961 caacgcatag agataaagcg tgccgccgcc cagccacatg ttcccgcttt gggcgatata
    37021 cagcagaatc gcgctaagcg gtgcggtggt gcatggtgaa cagatcagtc cggcaatcgc
    37081 ccccataaca aacacaccgc caggtgagcc gccctgttgg cgattgctca tcaacgtgag
    37141 acgtgtttgc agcgaagagg ggagttgtag agtaaacaag ccaaacattg acatcgccag
    37201 caaggtaaag acgatggcga ggccaatgag cacgtatggg tgctgtagcg ccgcctggaa
    37261 ctgtaaccct gcggcggcaa ctaccagacc cagcgccgtg taagtcagtg ccatcccctg
    37321 cacataaata aaggtcagca acaatgctct ggcagtggag agccgctgtt taccacccag
    37381 cacgatgcca gaaatcagtg ggtacattgg cagcacgcat ggcgtaaagg cgataccaat
    37441 accgatcaac aacgcccaga gcgcggaaaa gggcaattgc gcggtgtgct gctcttgctg
    37501 cgaaacagac acaggctgtg acgctgcgtt gttggcgacc acttcgctta acggaacggt
    37561 tttggtttct ggcggataac agaaaccggc atcagcacag ccctggtagg tgacagttaa
    37621 cgtcgctccc gcactcgcct ggttgatggt tacgggaagc gtcagccgat cgcggtaaat
    37681 ctcgcttttg ccgtaaaact catcttcatg ccagacgcct tgcggcagct gcacgtcggc
    37741 aattttcgcg tgttccggcg taatgcggat ctgtttacgg tagaggtagt aaccgtcttt
    37801 gatctgccag gtcagattca ggtcatgttg gttttgctga aaatcaaaag caaaggcttg
    37861 atccgcgggg acaaaatgtg aacgtcccgg cgcgtcgaat aatccggcaa aaacggaagt
    37921 gctgcaaagt agcaggatca gcgtaaagat gcgttgagcc atgagaggta atctgtgtct
    37981 ccgtgtgtaa caggtaaaac cagaagttcc ggggtttgat atggatgatg agacttcagg
    38041 cattccagca gtgcctgctg gtgagataca gtagttttta aaatcatctg cacttcgtat
    38101 tcttgctcca gcttaccttc ccagtaatag agagaggtag cgccggggat caaggtcgcg
    38161 caggccgcca gtttttccgc cagcactttg gcagctaaat cctgggctgt cgcttcatct
    38221 ggtgcggtac atagcaccac gacagacgcg gtattcgaac ttttttcatc aagcataaac
    38281 acctcgcaag aacagatgga accgcaagag agaggtcact atacaacgta cgggggaagg
    38341 atgttagtcg gcgggaaaga aagcggggcg tgaacgcccc gcgattgatc attacagcat
    38401 gaagctaccc agcacgaagc cgaagcaaac ggccagggca acacccagag tacccgggat
    38461 gaagaacgga tggttgaaga cgaatttacc gatacgggta gtacccgtgt catccatctg
    38521 taccgcagca accagcgtcg ggtaggtcgg cagaatgaac agaccagaca ccgcagcgaa
    38581 agaagcaaca gcggtcagcg gtgaaacgtt cagtgccaga gccatcggca tcagtgcttt
    38641 tgcggttgca gcctgagagt acagcagagc agaagcaaag aagaagatga cggccagcag
    38701 ccacggatga ccctgaatca cttcaccagc ggtatctttg atccagtcga tgttgttgga
    38761 aacgaaagta tcgcccagcc acgcaacacc caggatacaa atacaggcgc tcatacctgc
    38821 tttgaaggtg ctggagttga ggatgttgtc ggtatcgact ttacagataa cggtggtcag
    38881 agttgcaacg ctgagcatga tgatcaggat tgcgttggtg gtgttcatca gcggtttttc
    38941 aaccagaccc atgcttgggc tgttgatgat tgcatagata accacgccaa ctacgcccag
    39001 caggaacagc cagacggacg tttttgcacc ggatttgatt tcaatctgct tttcaccgcg
    39061 cagttcaacc aggccctctt ccagacgctt gcgataaatc ggatcgtcag agagtttgga
    39121 gttgaacagc atagtgacca gaaaggacat caccagaacc gccagcaggg tggacgggat
    39181 gaccacggag agcagatgga ggtagctgat gccatgacct tccatcacgg aagacatgta
    39241 aaccactgcc gctgagattg gcgatgcggt gatcgcaatc tgcgcggata ccactgcagt
    39301 agacagcgga cggcaaggtt tgacgccttg ttccttcgca acttcagcga taactggcag
    39361 tgtcgccaga gatatgttgc cagtaccagc aaagatagtc aggaaatagg tcacgatcgg
    39421 tgcgaggatc gtgatgtatt tcgggttacg gcgcagcagc ttttctgtct gatgaaccag
    39481 atagtccaga ccgccagcaa cctgcatggc agaaatagcg gcgataaccg ccatgataat
    39541 ggaaatgaca tcgaacggga tgttaccggg tttaacgcca atagcggcaa gaaccagcac
    39601 ccccaatccg cctgcaaaac caatacctat tccccccaat ctggcgccca agaagatcgc
    39661 cagcaaaact atgatgagtt ctacaactag catattagcc ttccttgttt tttaacaagt
    39721 tgatattagg ttgttatttt taagttactg ctcacaagaa aaaaggcacg tcatctgacg
    39781 tgccttcttt atttgtacta ccctgtacga ttactgttcg ctttcatcag tatagcgttt
    39841 tgctttgtaa gccgggtgca tcagattctg tacggagaaa atatcgtcaa gttccgcttc
    39901 agtcaacaga ccgcgttcca gaacgacttc acgtacactc ttaccggttt cggcacagat
    39961 tttacccacg atgtcaccgt tgtggtgacc gatgaacggg ttcaggtaag taacgatacc
    40021 gatagagttg taaacgtaac cttcgcacac ttctttgtta gcagtgatgc cgttaatgca
    40081 tttttccagc aggttgtagc aagcgttggt cagaatgtga acggattcga acatggcctg
    40141 gccaatgacc ggctccataa cgttcaactg cagctgacct gcttctgctg ccatggtaac
    40201 agtggtgtcg ttaccgatga ctttgaagca tacctggtta accacttccg gaacaaccgg
    40261 gtttacttta gctggcatga tggaagagcc cgcctgcagt tccggcaggt tgatctcgtt
    40321 caggccggca cgtggaccag aagagagcaa gcgcaggtcg ttacagattt tggacatctt
    40381 cacagccagg cgtttcagcg cgccgtgaac cataacataa gcgccgcagt cagaggtcgc
    40441 ttcgatcagg tcttcagccg gtacgcatgg gaagccagtg acttcagcca gttttttcac
    40501 tgccagcgga gagtactctt tcggcgtgtt cagaccagta ccgattgctg ttgcgccaag
    40561 gttaacttcc agcagcagtt cagcggtacg ttggatattt ttcacttctt ctttcagcag
    40621 gatgctgaaa gcgcggaatt cctgaccgag ggtcatcggt actgcgtcct gcagctgggt
    40681 acgacccatt ttcaggatgt cctggaattc gacagcttta cgttcaaagc cttcacgcag
    40741 ttggttaatc gcatctacca gcttaatcag ggaagagtaa actgcgatac ggaaaccggt
    40801 cgggtaggcg tcgttagtgg actgacattt gttaacatgg tcgttcgggt tcaggtactg
    40861 atattcacct ttctggtgac ccatcagttc cagaccgata ttggccagca cttcgttggt
    40921 gttcatgttt acggaagtac ctgcgccgcc ctggtagacg tctaccggga actgatccat
    40981 gcattttccg ttgttcagga cttcatcaca tgcggcaatg atggcattcg ctacactttt
    41041 aggaatggtt tgcagctctt tgtttgccat agctgcggct tttttaacca ttaccatacc
    41101 gcgaacaaat tcaggaatat cactgatttt gttgttgctg atatagaagt tttcaatcgc
    41161 tctcagagtg tgaacaccat agtaggcatc agctggaact tccctggtac ccaacagatc
    41221 ttcttcgata cgaatgttgt ttgacatgtg aaccttcttt ttcaagctgc caatgatttg
    41281 ctttaaacac acagaatata tgtggtttcg aatgtttttc gaccgacgat tatcccctgc
    41341 atcgaccgaa tacccgagat catatgctgc ttgaggattt ctaccgtaat ctggatcact
    41401 ttaagtgtcg gtttttaccc cttaattatt aatttgtgaa atagatcacc gctttgggat
    41461 tactaccaaa aatagttgcg caaacatctt gaaattttgc taatgaccac aatataagct
    41521 gaacgcgatt cgcaacccat tcaggtagcc gggttttacc ggctgctatt acaggagaaa
    41581 cctttgcgct ggttaccttt tattgccatt ttcctttatg tctatattga gatttcaatc
    41641 tttattcagg ttgcccatgt attgggggta ttgctgaccc tcgtgctggt tatattcacg
    41701 tcagttatcg ggatgtcact ggtacgtaac cagggcttta agaatttcgt gctgatgcag
    41761 caaaaaatgg cggcgggtga aaacccagcg gcggagatga ttaaaagtgt ttcgctgatc
    41821 attgccggtt tgctgctttt attaccgggc tttttcaccg acttcctcgg tcttctgctt
    41881 ttattaccgc cggtgcaaaa gcatctgaca gtgaagttga tgccgcattt gcgcttttct
    41941 cgtatgccag gcggcggttt tagcgccggg accggtggcg gtaatacttt tgatggtgag
    42001 taccagcgaa aggatgatga gcgcgaccgc cttgatcata aagacgatcg ccaggattaa
    42061 tgtcgaaacg ccggattatg tggttatgcc attttccggc gtttttcgtt ttggcagcaa
    42121 cagccataac cccgccagca tgatcagcgc atagagactt ttccagccga ccattgccag
    42181 taacagaacg cataacagcc cgccaaccac cgccagtagt cgataacgtc cttgcaataa
    42241 tttacagcct gccagcatgc ataacagata aatcataata aagatgccat tggcataaat
    42301 aataagagcg tccagattga tctctaaagc atgaatcacc aacgtgctca ccacacagca
    42361 gccgagcacc gcattgaggg cattattcgg gatatggcga gaagagaggc gtgccaggta
    42421 gtggtcagga ttatgttgcg cctgcgacca gaccaggcgg gcgaagctct gtatataaat
    42481 gttgagactg gcaaagcagg ccagatagcc aatcacgcag gcaatccata acgctcctac
    42541 accgaacaac tgcactacaa tttttggaag cgatgctgcc gccgccattt tttcaccata
    42601 ggcgtcgaag tgtaagacga ctaccgtaca gccccagtag actaatcctg ccagcagcag
    42661 accaatcatc aatgcgcgag gaaaatcacg ctctggattt ttaaattccg aggcaagatg
    42721 ggcaaatgct tccagaccga caaaacacca gaacatcact gataacgcag caaataaccc
    42781 ggtaagttcg atattaccag gtgccgggaa ggggatattc gcaggtttga tatcgcccac
    42841 ccaccagata gcgacaatca gcgcgacgat aagtccggca ataacggttt gtagattagc
    42901 actggaactg gcaccgcgag taccgatata ccacaccagc gccagcgtac cgagttctgc
    42961 caacaacagt tgccagctat gccagccaaa catcgcctgg ccgaacccgg cggcaatttg
    43021 tagtgcggca ggcaaaccca cgggaatgac cgataaaaac agccagccgg tgactcgctc
    43081 aagccgcgaa ccaaacgcca taccgacgaa gtgcgcgacg ccgcctgcgc tgggatagtg
    43141 gcgacccaga atcgcaaaca caatcgcaat cgggaacact aagataatca aaacgggcca
    43201 cgcccacagg ctgttattgc ctgctaccag tgcagctaac gcaggaacgg caaacacgcc
    43261 agtgcctaat aatgacgtcg atagcaggcc aatgccctgg gccagcccca gttcttgttt
    43321 gagtccactc atgggttgat gtccgattgc gcccaaattt tgggcaactg cgtagatttt
    43381 cgatggtagc acaatcagat tcgcttatga cggcgatgaa gaaattgcga tgaaatgtga
    43441 ggtgaatcag ggttttcacc cgattttgtg ctgatcagaa ttttttttct ttttccccct
    43501 tgaaggggcg aagcctcatc cccatttctc tggtcaccag ccgggaaacc acgtaagctc
    43561 cggcgtcacc cataacagat acggactttc tcaaaggaga gttatcaatg aatattcgtc
    43621 cattgcatga tcgcgtgatc gtcaagcgta aagaagttga aactaaatct gctggcggca
    43681 tcgttctgac cggctctgca gcggctaaat ccacccgcgg cgaagtgctg gctgtcggca
    43741 atggccgtat ccttgaaaat ggcgaagtga agccgctgga tgtgaaagtt ggcgacatcg
    43801 ttattttcaa cgatggctac ggtgtgaaat ctgagaagat cgacaatgaa gaagtgttga
    43861 tcatgtccga aagcgacatt ctggcaattg ttgaagcgta atcctcgcac gacactgaac
    43921 atacgaattt aaggaataaa gataatggca gctaaagacg taaaattcgg taacgacgct
    43981 cgtgtgaaaa tgctgcgcgg cgtaaacgta ctggcagatg cagtgaaagt taccctcggt
    44041 ccgaaaggcc gtaacgtagt tctggataaa tctttcggtg caccgaccat caccaaagat
    44101 ggtgtttccg ttgctcgtga aatcgaactg gaagacaagt tcgaaaatat gggtgcgcag
    44161 atggtgaaag aagttgcctc taaagcgaac gacgctgcag gcgacggtac caccactgca
    44221 actgtactgg ctcaggctat catcactgag ggtctgaaag ctgttgctgc gggcatgaac
    44281 ccgatggacc tgaaacgtgg tatcgacaaa gccgttaccg ctgcagttga agaactgaaa
    44341 gcgctgtccg taccgtgctc tgactctaaa gcgattgctc aggttggtac catctccgct
    44401 aactccgacg aaaccgtagg taaactgatc gctgaagcga tggacaaagt cggtaaagaa
    44461 ggcgttatca ccgttgaaga cggtaccggt ctgcaggacg aactggacgt ggttgaaggt
    44521 atgcagttcg accgtggcta cctgtctcct tacttcatca acaagccgga aactggcgca
    44581 gtagaactgg aaagcccgtt catcctgctg gctgacaaga aaatctctaa catccgcgaa
    44641 atgctgccgg ttctggaagc tgttgccaaa gcaggcaaac cgctgctgat catcgctgaa
    44701 gatgttgaag gcgaagctct ggcaactctg gttgttaaca ccatgcgtgg catcgtgaaa
    44761 gttgctgcgg ttaaagctcc gggcttcggc gatcgtcgta aagctatgct gcaggatatc
    44821 gcaaccctga ctggcggtac cgtaatctct gaagagatcg gtatggagct ggaaaaagca
    44881 accctggaag acctgggtca ggctaaacgt gttgtgatca acaaagacac caccaccatc
    44941 attgatggcg tgggtgaaga agctgcaatc cagggccgtg ttgctcagat ccgtcagcag
    45001 attgaagaag caacttctga ctacgaccgt gaaaaactgc aggaacgcgt agcgaaactg
    45061 gcaggcggcg ttgcagttat caaagtaggt gctgctaccg aagttgaaat gaaagagaaa
    45121 aaagcacgcg ttgaagacgc cctgcacgcg acccgtgctg cggtagaaga aggcgtggtt
    45181 gctggtggtg gtgttgcgct gatccgcgta gcgtctaaac tggctgacct gcgtggtcag
    45241 aacgaagacc agaacgtggg tatcaaagtt gcactgcgtg caatggaagc tccgctgcgt
    45301 cagatcgtcc tgaactgcgg cgaagaaccg tctgttgttg ctaacaccgt taaaggcggc
    45361 gacggcaact acggttacaa cgcagcaacc gaagaatacg gcaacatgat cgacatgggt
    45421 atcctggacc caaccaaagt aacccgttct gctctgcagt acgcggcttc tgtggctggc
    45481 ctgatgatca ccaccgagtg catggttacc gacctgccga aaaatgatgc agctgactta
    45541 ggcgctgctg gcggtatggg cggcatgggt ggcatgggcg gcatgatgta attgccctgc
    45601 acctcgcaga aataaacaaa cccccgggca gaaatgtcag ggggtttttc ttttggtcat
    45661 ctttctagta taagattcag acacggacga cgcgagtggc gtccagctca ttgattatgg
    45721 ggaataacat gcacgtaaaa tacttagcag ggattgtcgg tgccgcgcta ctgatggcgg
    45781 gttgtagctc cagcaacgaa ttgagtgctg ccggtcagag tgtacgcatt gtggacgagc
    45841 agccaggcgc agagtgccag ctgattggta ctgcgacagg taagcaaagc aactggcttt
    45901 ccgggcaaca cggagaagag ggcggttcta tgcgcggcgc agcaaacgat ctgcgcaacc
    45961 aggcggctgc aatgggcggt aacgtgattt atggcatcag cagcccgtcg cagggaatgt
    46021 tgtccagttt tgtcccgacg gatagccaga ttatcggtca ggtttataag tgcctgaact
    46081 gatgtgatcc agaggctggg tgacaaacgt atca
//
LOCUS       DAPGWD010000021        45421 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPGWD010000021 DAPGWD010000000
VERSION     DAPGWD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.021"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(349..435)
                     /locus_tag="RHX88_001103"
     tRNA            complement(349..435)
                     /locus_tag="RHX88_001103"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:Leu,seq:gag)
     gene            complement(450..782)
                     /gene="secG"
                     /locus_tag="RHX88_001104"
     CDS             complement(450..782)
                     /gene="secG"
                     /locus_tag="RHX88_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDV2229827.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1010..2347)
                     /gene="glmM"
                     /locus_tag="RHX88_001105"
     CDS             complement(1010..2347)
                     /gene="glmM"
                     /locus_tag="RHX88_001105"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDV2229828.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2340..3188)
                     /gene="folP"
                     /locus_tag="RHX88_001106"
     CDS             complement(2340..3188)
                     /gene="folP"
                     /locus_tag="RHX88_001106"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDV2229829.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3278..5212)
                     /gene="ftsH"
                     /locus_tag="RHX88_001107"
     CDS             complement(3278..5212)
                     /gene="ftsH"
                     /locus_tag="RHX88_001107"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDV2229830.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5312..5941)
                     /gene="rlmE"
                     /locus_tag="RHX88_001108"
     CDS             complement(5312..5941)
                     /gene="rlmE"
                     /locus_tag="RHX88_001108"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDV2229831.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6067..6360
                     /gene="yhbY"
                     /locus_tag="RHX88_001109"
     CDS             6067..6360
                     /gene="yhbY"
                     /locus_tag="RHX88_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDV2229832.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6516..6992)
                     /gene="greA"
                     /locus_tag="RHX88_001110"
     CDS             complement(6516..6992)
                     /gene="greA"
                     /locus_tag="RHX88_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDV2229833.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7240..8673
                     /gene="dacB"
                     /locus_tag="RHX88_001111"
     CDS             7240..8673
                     /gene="dacB"
                     /locus_tag="RHX88_001111"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV2229834.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8713..9885)
                     /gene="cgtA"
                     /locus_tag="RHX88_001112"
     CDS             complement(8713..9885)
                     /gene="cgtA"
                     /locus_tag="RHX88_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDV2229835.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9901..10866)
                     /locus_tag="RHX88_001113"
     CDS             complement(9901..10866)
                     /locus_tag="RHX88_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2229836.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTRG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(10993..11250)
                     /gene="rpmA"
                     /locus_tag="RHX88_001114"
     CDS             complement(10993..11250)
                     /gene="rpmA"
                     /locus_tag="RHX88_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDV2229837.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11271..11582)
                     /gene="rplU"
                     /locus_tag="RHX88_001115"
     CDS             complement(11271..11582)
                     /gene="rplU"
                     /locus_tag="RHX88_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDV2229838.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11841..12812
                     /gene="ispB"
                     /locus_tag="RHX88_001116"
     CDS             11841..12812
                     /gene="ispB"
                     /locus_tag="RHX88_001116"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDV2229839.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13040..13318
                     /gene="sfsB"
                     /locus_tag="RHX88_001117"
     CDS             13040..13318
                     /gene="sfsB"
                     /locus_tag="RHX88_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDV2229840.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13366..14625)
                     /gene="murA"
                     /locus_tag="RHX88_001118"
     CDS             complement(13366..14625)
                     /gene="murA"
                     /locus_tag="RHX88_001118"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDV2229841.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14680..14934)
                     /gene="ibaG"
                     /locus_tag="RHX88_001119"
     CDS             complement(14680..14934)
                     /gene="ibaG"
                     /locus_tag="RHX88_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDV2229842.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15094..15387)
                     /gene="mlaB"
                     /locus_tag="RHX88_001120"
     CDS             complement(15094..15387)
                     /gene="mlaB"
                     /locus_tag="RHX88_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDV2229843.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15387..16022)
                     /gene="mlaC"
                     /locus_tag="RHX88_001121"
     CDS             complement(15387..16022)
                     /gene="mlaC"
                     /locus_tag="RHX88_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDV2229844.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16041..16592)
                     /gene="mlaD"
                     /locus_tag="RHX88_001122"
     CDS             complement(16041..16592)
                     /gene="mlaD"
                     /locus_tag="RHX88_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDV2229845.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16597..17379)
                     /gene="mlaE"
                     /locus_tag="RHX88_001123"
     CDS             complement(16597..17379)
                     /gene="mlaE"
                     /locus_tag="RHX88_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDV2229846.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17387..18196)
                     /gene="mlaF"
                     /locus_tag="RHX88_001124"
     CDS             complement(17387..18196)
                     /gene="mlaF"
                     /locus_tag="RHX88_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDV2229847.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18406..19383
                     /locus_tag="RHX88_001125"
     CDS             18406..19383
                     /locus_tag="RHX88_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDV2229848.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19397..20383
                     /gene="kdsD"
                     /locus_tag="RHX88_001126"
     CDS             19397..20383
                     /gene="kdsD"
                     /locus_tag="RHX88_001126"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDV2229849.1"
                     /translation="MTHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20404..20970
                     /gene="kdsC"
                     /locus_tag="RHX88_001127"
     CDS             20404..20970
                     /gene="kdsC"
                     /locus_tag="RHX88_001127"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDV2229850.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            20967..21542
                     /gene="lptC"
                     /locus_tag="RHX88_001128"
     CDS             20967..21542
                     /gene="lptC"
                     /locus_tag="RHX88_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDV2229851.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21511..22068
                     /gene="lptA"
                     /locus_tag="RHX88_001129"
     CDS             21511..22068
                     /gene="lptA"
                     /locus_tag="RHX88_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDV2229852.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22075..22800
                     /gene="lptB"
                     /locus_tag="RHX88_001130"
     CDS             22075..22800
                     /gene="lptB"
                     /locus_tag="RHX88_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDV2229853.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22848..24281
                     /gene="rpoN"
                     /locus_tag="RHX88_001131"
     CDS             22848..24281
                     /gene="rpoN"
                     /locus_tag="RHX88_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDV2229854.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24304..24591
                     /gene="hpf"
                     /locus_tag="RHX88_001132"
     CDS             24304..24591
                     /gene="hpf"
                     /locus_tag="RHX88_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDV2229855.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24709..25200
                     /gene="ptsN"
                     /locus_tag="RHX88_001133"
     CDS             24709..25200
                     /gene="ptsN"
                     /locus_tag="RHX88_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDV2229856.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25246..26100
                     /gene="rapZ"
                     /locus_tag="RHX88_001134"
     CDS             25246..26100
                     /gene="rapZ"
                     /locus_tag="RHX88_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDV2229857.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26097..26369
                     /gene="npr"
                     /locus_tag="RHX88_001135"
     CDS             26097..26369
                     /gene="npr"
                     /locus_tag="RHX88_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDV2229858.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26583..27215
                     /gene="yrbL"
                     /locus_tag="RHX88_001136"
     CDS             26583..27215
                     /gene="yrbL"
                     /locus_tag="RHX88_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDV2229859.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27212..27940)
                     /gene="mtgA"
                     /locus_tag="RHX88_001137"
     CDS             complement(27212..27940)
                     /gene="mtgA"
                     /locus_tag="RHX88_001137"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDV2229860.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(27937..28590)
                     /gene="elbB"
                     /locus_tag="RHX88_001138"
     CDS             complement(27937..28590)
                     /gene="elbB"
                     /locus_tag="RHX88_001138"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDV2229861.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28820..31156)
                     /gene="arcB"
                     /locus_tag="RHX88_001139"
     CDS             complement(28820..31156)
                     /gene="arcB"
                     /locus_tag="RHX88_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDV2229862.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31252..32181)
                     /locus_tag="RHX88_001140"
     CDS             complement(31252..32181)
                     /locus_tag="RHX88_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDV2229863.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            32856..37316
                     /gene="gltB"
                     /locus_tag="RHX88_001141"
     CDS             32856..37316
                     /gene="gltB"
                     /locus_tag="RHX88_001141"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDV2229864.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37329..38747
                     /gene="gltD"
                     /locus_tag="RHX88_001142"
     CDS             37329..38747
                     /gene="gltD"
                     /locus_tag="RHX88_001142"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDV2229865.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            38931..39410
                     /locus_tag="RHX88_001143"
     CDS             38931..39410
                     /locus_tag="RHX88_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDV2229866.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            39427..39732
                     /locus_tag="RHX88_001144"
     CDS             39427..39732
                     /locus_tag="RHX88_001144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2229867.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(39808..40272)
                     /locus_tag="RHX88_001145"
     CDS             complement(39808..40272)
                     /locus_tag="RHX88_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV2229868.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(40269..41144)
                     /gene="nanK"
                     /locus_tag="RHX88_001146"
     CDS             complement(40269..41144)
                     /gene="nanK"
                     /locus_tag="RHX88_001146"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDV2229869.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHLSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(41141..41830)
                     /locus_tag="RHX88_001147"
     CDS             complement(41141..41830)
                     /locus_tag="RHX88_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDV2229870.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(41878..43368)
                     /gene="nanT"
                     /locus_tag="RHX88_001148"
     CDS             complement(41878..43368)
                     /gene="nanT"
                     /locus_tag="RHX88_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDV2229871.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(43477..44370)
                     /gene="nanA"
                     /locus_tag="RHX88_001149"
     CDS             complement(43477..44370)
                     /gene="nanA"
                     /locus_tag="RHX88_001149"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDV2229872.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(44492..45283)
                     /gene="nanR"
                     /locus_tag="RHX88_001150"
     CDS             complement(44492..45283)
                     /gene="nanR"
                     /locus_tag="RHX88_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDV2229873.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        11175 a        12338 c        11316 g        10592 t
ORIGIN      
        1 ttttcttttt aaatgatgcg gccattgttc tcagtaatta aataacatta tcaatgcgtc
       61 tctaaacatt acgcagaacg tagccatcag tagttatact gacaggagca ggatggttta
      121 tcgagaagta aacatatccg tattcgtacc ggaactggag ttgacacaat aaagtgccaa
      181 ttatgtcagc agaagggaaa agtttataac taaggcgtac cggcaccatc gtttcaaggt
      241 accagctacg agtaaagcaa ctggacgaga tacagatacc tgacaaccat tcctcagacc
      301 aggaccaaaa cgaaaaaaga cgcttttcag cgtctctttt ctggaatttg gtaccgagga
      361 cgggacttga acccgtaagc cctattgggc actaccacct caaggtagcg tgtctaccaa
      421 ttccaccacc tcggcacgga tactactttt tagttcggga tatcgctggt cggcttagcc
      481 ggagcagctg gttgagtttg ttcggttttc accggtgcac tcagattttc ccattcgcta
      541 cctttattgg ttttgttgct attgatgtta cccagcacca gactgatgat gaagaataac
      601 gttgccagca gcgccgtcat gcgggtcatg aagttaccag aaccacttga accaaacagc
      661 gtagcggaag cgcctgctcc gaaggaggct cccatatcag cgcctttacc ttgctgcagc
      721 atgatcagac caacaaggcc aattgccaca ataaggaaaa ctactaaaag agcttcgtac
      781 ataatcaacc tgttccttgc ggagttgccg cgtaccaatg cttcaaccaa taaagcggga
      841 gtttttaatc tttcccactg aagcgggtgt gaatactaac caaagcgaat gaccttcgca
      901 agggcaattt tatcgcattg tatcaactgc ggaaaaaaac agcaaaaccg atgtgttcgc
      961 tgagaaaaaa ggcgacaatc gccgcctttt tagccagtta tctaacgctt taaacggctt
     1021 ttactgcatc ggcgatgcgg tgtgcaaatt cagtcacctg cgcttcgtct tcgccttcca
     1081 ccatcacgcg aattaacggt tcggtgccgg atttacgcag caacacgcgt ccacggttgc
     1141 ccagcgcagc ttcaacctct gcggtcacgg ctttaactga ctcatgctca agtggatcgc
     1201 cgctacctgc ggtgtaacgt acgttaacca gaatctgcgg gaacattttc atgccgctgc
     1261 aaaggtcgtg caggctcata tggttacgtg ccatcgccgc cagcacctgc aagccagcaa
     1321 cgatgccgtc accggtagta gttttatcca gtaggatcac atgaccggaa ttctctgcac
     1381 cgatacgcca gcctttctcc tgcatttttt ccagtacgta gcggtcaccc actttcgcgc
     1441 gcgcaaatgg aattcctaac tgtttcagcg ccagttcaag ccccatgttg ctcatcaatg
     1501 tacccacagc gccaccacgc agctggccct gacgaagacc ttcacgcgcg atgatataca
     1561 tgatctgatc gccatcgact ttattgcctt catggtcgac cataatcacg cgatcgccat
     1621 cgccgtcgaa ggcaataccg agatccgctt tttcagccag cacacgagcc tggagcgcgc
     1681 gaacgtcggt agcccccact tcggcattga tgtttacacc gtttggctca caaccgatag
     1741 cgataacgtt cgcccccagt tcgcgcagca cgttcggcgc gatgtgataa gtcgcaccgt
     1801 ttgcacaatc caccacaatc ttcagttcac tgaggctaag ttcgttcggg aacgtggctt
     1861 tgcaaaactc gatatagcga cccgcggcat caacgatacg gctggcttta cccagttctg
     1921 ccgaatcaac gcagctgatc tccttttcca tttccgcttc gatggcctct tctaccgcat
     1981 ccggcagttt ggtgccgtcg atagagaaga atttaatccc attatcgtag aacgggttat
     2041 gcgatgcaga tatcacaatt ccggcctctg cgcggaaggt acgcgtcaga taagccacgg
     2101 ccggtgttgg catcgggcca gtgaagagtg cggaaaggcc cgctgccgcc agacccgctt
     2161 ccagtgctga ctccagcata tagccagaaa tacgcgtgtc tttaccaata ataatcttac
     2221 gggagccgtg gcgcgccagc actttacccg cggcccaacc cagcttaagc acaaaatcag
     2281 gtgtgatcgg cgcatcccct acacgaccac gaatcccatc ggtaccgaaa tatttacgat
     2341 tactcatagc gtttgttttc ctttgcagac agagtggctt ccaccacccg catcgcttct
     2401 acggtttctt tgacgtcatg aacacgaatg atgtgcgcgc cttgcattgc ggcaatgacc
     2461 gcacaggcca gactaccgct caggcgctcg gacggcccca cgttcagcag ctgcccaatc
     2521 atcgattttc gtgacatacc caccaacagc ggcaggttga aatggtgaaa ttcagccagg
     2581 cgcgccagta atgaatagtt atgggagaga tttttaccga aaccgaatcc ggggtcgagc
     2641 aacaatttct cttttgcgat acccgcctgc tcgcaacgtg ctatttgctc aataaagtag
     2701 cgattcactt ctgcaaagac atcgtcatac ttcggagctt cctgcatggt ttttggattt
     2761 ccctgcatat gcatcagaca aaccggtaaa ccggtttctg cagccgcctc cagagcgcca
     2821 ggttcggaaa gggagcggat atcattaata atgtgagcgc caactttcgc tgactcacgg
     2881 atgacttctg gtttggatgt atcgaccgag atccagactt cgaagcgttg agcaattgcc
     2941 tcaaccacag gaataacacg ttgcaactct tcttcaacgc taacttccgc cgcccctggg
     3001 cgcgtggact cgccaccaac gtcaatgatc gtcgcgccag cattgatcat cagattcgca
     3061 tgtttcaccg catctatcag cgagttatgc gtgccaccat ccgaaaagga atcaggcgtg
     3121 acgttgagga tccccattac gtgaggatgg ctaaggtcca gtgaagtacc ctgggcaaag
     3181 agtttcatga tgttatccct ggtatgaatt gataagaaaa aaccccggag cacgccccgg
     3241 ggttttcggt acaaatacag tcatctgatg cgggaactta cttgtcgcct aactgctctg
     3301 acatggtgtt acccgggttc ggcgtacgcg gttcatcaac cggacgagga gcctttggac
     3361 taccattgtc gccagaattg ttagaagcgc ctggttcttc ccagcccgct ggcggacgta
     3421 catcgcgacg tgccatcagg tcatcaatct gcggtgcgtc gatagtctca tatttcatga
     3481 gagcatcttt catcgcatgc agaatatcca tattgtcggt cagaagctga cgcgcacgat
     3541 tatagttacg ctcaatcagt gctttcactt cctggtcgat gatacgtgca gtttcatcgg
     3601 acatatgttt cgctttcgct acgctacggc cgaggaatac ttcaccttct tcttccgcgt
     3661 acagcagtgg acccaatttc tcagagaagc cccactgggt caccatgtta cgtgccagat
     3721 tggtcgcaac tttaatatcg ttggacgcac cggtagaaac atgttccggc ccatagatga
     3781 tctcttctgc cagacgacca ccgtacagcg tagaaatctg gctttccagt ttctgacggc
     3841 tggcgctgat tgcgtcgccc tcaggcaaga agaaagtcac acccagcgca cgaccgcgtg
     3901 ggataatcgt cactttgtgc accggatcgt gttccggcac caggcgaccg ataatcgcat
     3961 gacccgcttc gtggtaagcc gttgattctt tctgcgcttc cgtcatcacc atggagcgac
     4021 gttccgcacc catcatgatt ttgtctttcg ctttctcgaa ctcaaccatc gacacaacgc
     4081 gtttgttgcc acgagcagcg aacagtgccg cttcgttcac caggttcgcc aggtcagcac
     4141 cggagaaacc aggagtacca cgggcaatga ttgccgcgtc gatatcgggg gccaatggta
     4201 cgcgacgcat gtgaactttc aggatctgct cacgaccgcg aacatctggc aagccgacca
     4261 caacctgacg gtcgaaacgg ccaggacgca gcagggccgg gtcgagaacg tccggacggt
     4321 tagtcgcggc gataacgatg ataccttcgt taccttcgaa gccatccatc tcaaccagca
     4381 tctggttcag agtctgttca cgttcatcgt gaccaccgcc cagaccagcg ccacgctggc
     4441 ggcctacggc gtcgatttca tcgataaaga tgatgcacgg tgccgctttc ttcgcctgtt
     4501 cgaacatgtc acgaacacgg gatgcaccca caccgacgaa catttctacg aagtcagaac
     4561 cggagatagt aaagaacgga actttcgctt cgcctgcaat cgctttcgcc agcaacgttt
     4621 taccggtacc cggaggaccg accatcagga cgcctttcgg gatcttaccg ccgagtttct
     4681 ggaagcggct cggctcgcgc agatactcta ccagttcagc aacttcttct tttgcttcgt
     4741 cgcagcccgc aacgtcagca aaggtcgttt tgatctgatc ttccgtcagc atgcgcgctt
     4801 tgctcttacc aaacgacatg gcacctttgc caccgccgcc ctgcatttga cgcatgaaga
     4861 agatccagac accaattagc aacagcatcg ggaaccagga gatgaagata gaagccagca
     4921 ggcttggttc ttcaggcggt tcaccgacaa ccttgacgtt cttggtcaac aggttatcca
     4981 gtaatttcgg atcctgaacc ggaatgtaag tggtataacg gttactatct ttcttggtaa
     5041 cgttgatttc acgtccgttg atacgcgctt cacgaacctg gtcgttattg acctcttgta
     5101 ggaaggtaga gtaatccacc ttacggccat tagactcgct gggcccaaag ctctggaata
     5161 ctgacatcag cacaacggca atgaccagcc agagtattag gtttttcgcc atgtcactca
     5221 agggattaac ctcttattac aactgtgtta aaaacagcgt caggatactc tatatccagc
     5281 atctttcaaa ctttcgtctg aaatctcccg gttagggttt acgcccggtc gctacaatat
     5341 acacttcccg cgaacgtgca cgagaagagt ccggcttacg aactttgacc ttcgtaaaca
     5401 gggagcgaat ttcccttaga tactcatcga aaccttcgcc ctggaacacc ttcactacaa
     5461 aactgccacc tggcgctaat acatcacgac acatttctaa cgccagttcc accagataca
     5521 tggcacgggg gatatccacc gccggtgttc cgctcatgtt tggtgccata tcggacatga
     5581 caacctggac tttgctgtcg ccaacgcgct ccagcagtgc tttcatcacc agttcatcac
     5641 gaaaatcgcc ctgaagaaag tccacaccaa cgataggatc cataggtaaa agatcgcaag
     5701 cgatgatgcg gcctttgccg ccaatttggg tgaccacata ttgtgaccaa ccacccggag
     5761 cagcaccaag gtcgacaacc gtcattcccg gtttaaagag tttgtcactt tgctgtattt
     5821 catcaagttt aaaccaggca cgggaacgta accccttttt ctgtgcctgt tgaacatatt
     5881 tatcgctaaa gtgttcctga agccagcggc tggagctggc agaacgcttc ttacctgtca
     5941 tttaactttc ccatggggat aactcatcgt aaccaattgc gtaaattttt acgcgcctat
     6001 ttggtgatat atgggagatg gcggtagaat gacccgtttt caatcccaac gtaagcaaaa
     6061 aatacgatga atctgagtac taaacaaaaa cagcacctga aaggtctggc acatccgctc
     6121 aagccagttg ttctgcttgg cagtaatggt ttgaccgaag gggtgctggc cgagattgaa
     6181 caagcgttag agcaccatga actcatcaag gtgaaaatcg ccaccgaaga tcgcgaaact
     6241 aaaaccttga tcgtggaagc tatcgtgcgc gaaaccggcg cctgtaatgt acaggtcatc
     6301 ggtaaaacgc tggtgcttta tcgcccaact aaagaacgta aaatctcgct gccacgctaa
     6361 gattatccta aagttacaca catttgctgt gtaaaacgag gggttttccg caggcaggag
     6421 agcaaaatgc cacgctctgt tcgttgataa aaggccgcat agcggccttt ttcctttctt
     6481 tacaatacat caacatcttg agtattgggt aattcttaca ggtattccac cttaattact
     6541 tcaaattcta cttcgccgcc cggcgttttg atgaccacaa catcatcttc ttctttgccg
     6601 atcaggccac gagcaatagg cgagtttaca gaaatcaggt tttgtttaaa gtcagcttcg
     6661 tcatcgccaa cgatgcgata agtctgttct tcgtcagaat ccagattcag caccgttacg
     6721 gtagcaccaa aaataacgcg cccattgttg ggcattttgg tgacatcaat cacctgcgcg
     6781 ttcgacagct tggcttcgat gtctttaata cggccttcgc agaaaccctg ctgttcacga
     6841 gctgcgtggt attcggcgtt ttctttcagg tcgccatgct cacgcgcttc cgcgatagca
     6901 gcaatgattt caggacggcg cacagatttc agaaaatcca gctcttcgcg taatttttca
     6961 gcgccgcgta aggtcatcgg aatagcttgc atttgttata cctcttgaat attcctgatg
     7021 gggcaagtct tcacccaccc cggctgttcg gcctgcccgg cataactcct caccgggacc
     7081 agaagcaaaa aaataccgac ccgggtacaa gtcccaggtc agctacaatt cacattttga
     7141 tagtcatttt accctgaagt tcccgaaggg tcatcgttta ctttataggg cgttgcgccg
     7201 tagtatgacg gctcgattcc aggttgttag cgcgagatta tgcgattttc cagatttatc
     7261 atcggattga ccagctgtat agcgttcagt gttcaggccg cgaatgttga tgagtacgtt
     7321 actcaactcc ccgctggtgc caaccttgcc ctgatggtgc aaaaagtcgg cgcgtcggcc
     7381 cccgctattg attaccacag tcagcagatg gcgctgcccg ccagtacgca gaaagtgatt
     7441 actgcgctgg cggcgttgat tcaactcggc cccgattttc gtttcaccac gacgcttgaa
     7501 accaaaggca atgtggaaaa cggcgtactt aagggtgact tagtggcgcg atttggtgcc
     7561 gatccgacgt taaaacgtca ggatattcgc aatatggtcg cgactttgaa aaaatctggc
     7621 gtcaaccaaa tcgatggcaa tgtgttgata gatacctcca ttttcgccag ccacgataaa
     7681 gcccccggct ggccatggaa tgacatgaca caatgcttta gcgctccgcc tgccgctgcc
     7741 atagttgacc gcaactgttt ctccgtctcg ctctacagtg ccccaaaacc tggtgatatg
     7801 gcttttatac gcgtggcatc ttattacccc gttacgatgt tcagccaggt acgcaccctc
     7861 ccccgaggtt ctgccgaagc gcaatactgc gaactggatg tcgtaccagg cgacctgaac
     7921 cgctttacgc tgacgggatg cctgccacaa cgttctgagc cgctcccgtt ggcttttgcc
     7981 gtgcaggatg gagccagcta tgccggggca attctgaaag atgagttaaa acaggcaggt
     8041 atcacctgga gcggaacact gctgcgccag actcaggtta acgaacctgg aacggtagtt
     8101 gccagtaaac agtcggcccc gttgcacgat ctgcttaaga ttatgctgaa aaagtcggac
     8161 aacatgatcg ccgatacggt tttccgcatg ataggccatg cgcgcttcaa tgtgcctgga
     8221 acatggcggg ccgggtcgga cgccgtgcgt cagatcctgc gccagcaagc cggtgtcgat
     8281 attggaaaca ccattattgc cgatggttca gggctttcgc ggcataacct gattgccccc
     8341 gccaccatga tgcaggtgct gcaatacatt gcccaacacg acaatgaact taactttatc
     8401 tccatgctgc cgcttgcggg ctatgacggc tctttgcagt accgtgcagg tctgcatcag
     8461 gcgggcgtgg atggaaaagt ctcagcgaaa accggttcgt tgcagggggt atataacctg
     8521 gcgggattca ttaccacagc gagcgggcaa cgaatggcgt ttgtacaata tctttctggc
     8581 tatgcagtag aaccggcgga tcagcgtaat cgccgtattc cgttagtgcg ttttgaaagc
     8641 cgtttgtata aagatattta tcagaacaat tagtcaaaaa gaaaccccgg cacatggccg
     8701 ggtttttgct gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca
     8761 gtcatcatcc cagtcctcat catcctcttc agcaatctct tcaagctgct ggcgatgata
     8821 atcatcccac atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg
     8881 gttttcaatg ataaaggtca tcacatccca gcagagatct ttcacgccca gtccactcgc
     8941 cgcagagatc agataatatt tatcttccca gcccagcgct tcagcaatcg ctttcgcttt
     9001 ctcttcagct tctgccttat ccagcagatc gatcttattg aacactaacc aacgtggttt
     9061 cgccgccaga tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac
     9121 cggatcggtg ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc
     9181 caggtgcttc aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc
     9241 tggaatatcg gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg
     9301 caccagagtg gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat
     9361 aaaggtcgat ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag
     9421 ctccagcagc agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg
     9481 gttaactgac gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag
     9541 cagacgctga ccgtgtttgg tcatatcgcc catggtttca ccagtaccct ggtcgattac
     9601 acgcgtacct accggcactt taattgtgac gtctttaccg cgcttaccgg tacagtcgcg
     9661 gcttgcgcca ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag
     9721 cgtgttcagg ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc
     9781 atccgggccg cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc
     9841 atcacctgca acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa
     9901 tcattcgcct gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat
     9961 acgatgacca atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc
    10021 taaaaggttt aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga
    10081 aaaaaacagc gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca
    10141 gcgagccatc gcttccgcca gggcgccata tcctaccaat gtattcagtc cgcaaaaaat
    10201 taaacatgcg agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa
    10261 gagcgcaatt gtacataaag cgtacagtaa aaacaggatc tgcggtgagg ccagccgacg
    10321 caataaaacc ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa
    10381 gatcactccc ctggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa
    10441 aaacatcacc aggccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt
    10501 ctctttcagg ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac
    10561 ctgcgaagcg gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa
    10621 cagcccggcg gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa
    10681 tggcggcaac ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa
    10741 acggtaaaac acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg
    10801 caacgccccc cagcaaattg ctgtggtgag cgccaaaaga atgccaatgc ctgcctgctg
    10861 cttcataccg tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt
    10921 taccggttat cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg
    10981 gacgcgaaaa acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac
    11041 ttcgaatttc actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac
    11101 gttagcgcca gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac
    11161 ggattcgcca ccgaaacgct taacgcccag gcgtttagct tctgaatcgc gaccgttacg
    11221 tgtggagccg ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg
    11281 atgccagtaa ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt
    11341 ttacgacgac gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca
    11401 gctttgatta cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg
    11461 atcatcagca cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg
    11521 cgaacggtct gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac
    11581 atataaaact ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc
    11641 acaatagggc gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca
    11701 cgaagaaaaa aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat
    11761 cactctatat tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg
    11821 cgaaaagccc ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata
    11881 tggcgggtgt taatgcggca atccttgagc agcttaattc cgacgtccag ctgatcaatc
    11941 agttaggcta ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac
    12001 tggctgcacg agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg
    12061 agtttatcca cacggcgact ctgctgcacg acgacgttgt ggatgaatca gatatgcgca
    12121 ggggtaaagc taccgccaac gctgcatttg gcaatgccgc cagcgtgctg gtaggcgatt
    12181 ttatttatac ccgcgcgttc cagatgatga ccagcctcgg ttcgctcaaa gtgctggaag
    12241 tcatgtcaga agccgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta
    12301 acgatccgga catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc
    12361 tgtttgaggc tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaagaggaga
    12421 aaggcctgca ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac
    12481 tcgattacaa tgccgatggc gaacagttag gtaaaaatgt cggcgacgat ctgaacgaag
    12541 gtaaaccgac gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga
    12601 tgatccgtac cgccatcgaa cagggtaacg gtcgccatct tctggaaccg gttctggaag
    12661 caatgaacgc ttgtggatct cttgaatgga cgcgtcagcg tgccgaggaa gaagcagaca
    12721 aagccatcgc agcattacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc
    12781 tcgcgcacat cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc
    12841 gggatgattc cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaaa
    12901 gggctgtgca tctttatata ttctgaatat tcacactttt tacaggaact ttttagagca
    12961 ataggccatc aggagtatag tgatgcccga cagaagaagt gttctgaatg aaagcgacta
    13021 acttaaggag tgaggaaaga tggaaagtaa tttcattgac tggcatcccg ctgacatcat
    13081 tgcgggtttg cgcaagaagg gaacgtcaat ggcggcggag tctcgcagaa atggtttgag
    13141 ttcctcaacg ctggcaaacg cattatcgcg cccatggccg aaaggagaga tgattattgc
    13201 gaaagccctg ggaactgacc cctgggttat ctggccatca cgctaccatg atccgcagac
    13261 ccatgagttt atcgacagaa cgcagttgat gcgtagctac actaaaccga aaaaatgagt
    13321 ggtctggcgg tagccccgcg aacggggctg ccagctctca gacgattact cgcctttcac
    13381 acgctcaata tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt agccacgatc
    13441 gatgtgataa atacgatcaa ccaccgtcgt cccttccgca atacagccag ccagcaccag
    13501 gcttgctgat gcacgcagat cggttgccat aacctgtgcg ccagaaagtt tttcaacacc
    13561 gtgacaaata acggtattgc tttcgatttc ggcatgcgcg cccatacggc taagctctgg
    13621 cacatgcata aagcggtttt caaagaccgt ttcggtgatg aatccggtcc cttctgccac
    13681 caggttcaac agcgtgaact gggcctgcat atcggtcggg aatgccggat gcggcgcggt
    13741 acgtacgtta acagccttcg gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc
    13801 gacttcgatg tccgctccag cgtcacgcag tttcgccagc acggcgtcga gagtatctgg
    13861 ctgcgcgtta cggcagataa ttttgccgcg agaaatcgcc gccgccacca ggaaagtacc
    13921 ggtttcgata cgatccggta gcacgcgata gacaccgccg cctaaacgtt ccacaccttc
    13981 gatgacgata cgatcggtgc cctgaccgct aattttcgca cccagcgtaa tcaggaagtt
    14041 cgcggtatcg acgatttccg gttcacgcgc tgcgttttca ataatcgtgg taccttccgc
    14101 cagggttgca gcacacatga tggtcaccgt tgcgccaacg ctgactttat ccatcacgat
    14161 atgtgcgcct ttcagacgac catcgacgga agctttaacg taaccttctt ccagtttgat
    14221 ggtcgcgcct aattgttcga ggccagaaat gtgtagatca accggacgcg caccgatcgt
    14281 acaaccgcca ggcagtgaaa cttgcccctg accaaagcgc gctaccagcg gccccagtgc
    14341 ccagatagaa gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata cattaacgtc
    14401 gcgggcatca atatgcacag aaccattacg ttctactttc gcacccagct ggcttagcag
    14461 cttcattgat gtatcgacgt ctttcagttt tgggacgttc tggatctcta ccggttcttc
    14521 cgccagtaga gcggcaaaaa ggataggcag agcagcattt ttagcgccgg aaattgtgac
    14581 ttcgccctgg agcttcgttg gcccctgaac acgaaattta tccatttagt ttgttctcag
    14641 ttaacaattc atatccgcta ccggcgaatc gcccatagct caaaagccgt tcagtttgcg
    14701 atcgcgcgcc cactccgcag gggtatacgc tttgatcgac acagcatgaa tgcggttatc
    14761 cgcaatatat tccatcagcg ggccatagac cgtctgctgt tttttaaccc gactcatgcc
    14821 gtcaaacaac tcacccacgg caataacctg aaagtggctg ccatcgccgg aaacgtggac
    14881 ttcctggagg gagagagcgt tcatcaacac gctctgaatt tcattatttt ccatgggatc
    14941 ttcaatcatc agttaataaa ccagcgaaac atcttagagc aaagttgcgc tggcataaat
    15001 aagcaaaaag cctcgctgat aaatcagaca aggctcgact tgcaggcagg tttgccggac
    15061 aggcggttaa cgccatatcc ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa
    15121 attatacaat ttcgcgaggg tatacacttt gtcgtttacc ccctgaagcg tcacgttgtt
    15181 gccctgcttt ttcgccagat cgataagatg gagcagcagt gccagtccac ccgtatccac
    15241 gcgggacaca cggctaagat cgatgcaggt aatccccttc accgcttcct cacgcatttc
    15301 ccaaagcggt agcagaacgt cctgatccag ctctccggat aacgccagcg tgtcacccgt
    15361 ctgcatccag ctcagtgact cgctcattat tttttctctt ccagagtgat tttctgttga
    15421 gaaatcgatt tcagttgcgc agtcaggccg tcgatacctt tggtacgcag cagcgttccc
    15481 cactcgtttt gtttggtggt gatcatactg acgccttcag caatcatgtc gtaagcctgc
    15541 cagttgcccg tctgggagtt tttacgccac tggaagtcca gacgcaccgg cggacggcca
    15601 ttcgggtcaa taatggtaac gcgaataggc acaatggttt tatcgcccag cggctgttct
    15661 ggcgcaatct gataggtttg accgtgatac atcgccagcg cctgaccgta agcctgcttc
    15721 aggtactcac ggaaagcggc aaagtaggct tcacgttgag caggggtcgc actcttgtaa
    15781 tactggccca gcaccagcgc accggcgtat ttcacctgta cgtatggcag cagttcctga
    15841 tcaacaatgg tacgcagata atccgggttg gcccgaattt gcggttgctc attcttcagg
    15901 cgatcgaacg ttttctgcgc cgcctcgtcc atcagcttat aaggattggt ctggtctgcc
    15961 gcggttgccg cactcagagg tgcaatcacc agcaaagcga ccatcattaa acgtttaaac
    16021 atgcgtcggt tctcctgaaa ttatttcgtt gtacccacag gttcagtggt ttcattatta
    16081 cctggcgcag cagctggcgc atcgccacta ttcttattgt catcgccttt actaccgtaa
    16141 aggaactgac caatgagatc ttccagcacc atcgcagact tagtgtcctg aattgtatcg
    16201 ccatccttca ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt taatgccaga
    16261 tattgttccc ccagcaggcc ggaagtacga atgctcagcg aactggtatc tggaatgtgg
    16321 ttataacgtt gttcaatttc cagcgttacg cgcggcagat aggttttcgg gtccagcgta
    16381 atatccgcca cccgacccac aacaacgcca ccaatactga ccggagagcg cgctttcagg
    16441 ccgccaatgt tatcgaacgt cgcataaagc gtgtaggtcg gttcagtacg tatggacgtc
    16501 acgttcgccg ccttcaggca aacaaacagc gccgccagca gtgctgctaa taaaaagata
    16561 cccacccaaa tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt
    16621 cagcacaaaa tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc
    16681 ccggctaatc ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt
    16741 caccgtgatg gcgaacacca cgctcttaat cagacagttg accagatcca tacgccagtc
    16801 gacggcattt tgcattgccg accagaagaa cccgctatca atgcctttcc aactgacgcc
    16861 gaccagagat ccgccccaga tccccacggc gacgaaaata accgtcaaca gtggtaatga
    16921 aataacccca gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat
    16981 ctccatactg gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttagcgccga
    17041 accagcacgc ccggcaaaca acaacgcggc aaccaccggc cccagttcac gcagtagcga
    17101 taacgccacc agcataccca gactggtttc cgcactataa gtggtcagaa ccagataacc
    17161 ttgcagcccc aacaccattc cgatgaacac gccagaaacc acaataatca gcatcgacag
    17221 gacgccgaca ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa attccggttt
    17281 gccgaccagc gcattgaata acattaaccc ggcccgcccg aacgttctca gggttttaat
    17341 ccctttatgt ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta
    17401 aaagatcagc gtgataatcg ccggcaggat agcggaacgg aacaggcccg tcagctatcc
    17461 cgtccagaaa ctgacgtacg cgcggatcag gattcgcctg caacgcctgg gcactgccat
    17521 gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt aacacttccg
    17581 gcacatcgtg agaaaccacc acacaagtca cgcccagcgc gctgttcagc tcagaaatca
    17641 gcttcaccag tacgcccatg gtgatgggat cttgcccaac aaaaggttca tcaaacatga
    17701 tgagatccgg ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc atcccaccag
    17761 aaagttcaga aggcattagc ttagccgctc cacgcagccc cacggcctcc agcttcatca
    17821 tcactgtact atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga
    17881 cgttgtcaaa tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc
    17941 gtttgcgcac tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa
    18001 ggatctcacc atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac
    18061 cgatgcccga tggccccatg attgccgtga tcttccctcg cggcacggtc agggaaatat
    18121 tatcgaagat gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg
    18181 ccacagactg ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca
    18241 atttaacctt gaacccaaca tatttacaga atattacccg ccgtggttag cgaaagctgg
    18301 catttgtttt actttttagc cgcataaagt caaaattaag aatccgttac ggctttctga
    18361 aaatcttcag cggaccggcg agtatacctg aagaaaggac gttagatgct tttagctacg
    18421 gcactgttaa ttgttggttt acttttggtc gtttacagtg ccgaccgcct ggtttttgcc
    18481 gcgtctattc tttgccgaac ctttggcatc ccgccgctga tcatcggcat gacggtggtc
    18541 agtattggta catcgttacc agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa
    18601 cgcgatttag ccgttggtac agccctcggc tcaaacatta tcaatatatt gctgatcctc
    18661 ggtctggctg cgctggttcg tccttttacc gtccattctg atgttctacg ccgtgaatta
    18721 cccttaatgt tgttggtcag cgtggtggcc ggttccgtac tctatgacgg acaacttagt
    18781 cgcagcgatg gtatctttct cctctttctg gctgtgctat ggctgctgtt cattgttaaa
    18841 cttgcacgtc aggctgaacg tcaggggact gacagcctga ccagagagca gcttgcagag
    18901 ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc tcggcattgc gcttatcatc
    18961 atgccagtgg ccacgcggat ggtggttgat aacgccacgg tgctggcgaa ttactttgcc
    19021 atcagcgagt tgacgatggg tctgacggca attgctatcg gaaccagcct gccggaactg
    19081 gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca ttgctgtcgg aaatatcatt
    19141 ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat aacgccagga
    19201 gagattgatc cactggcgta cagtcgtgac tacagcgtga tgttgctggt gagcattatt
    19261 tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc gtggtgtagg ggtattatta
    19321 actggcggat ttatcgtatg gctggcgatg ttgtactggt tatcgccaat actcgttgaa
    19381 taactggaaa cgcattatga cgcacgtaga gttacaaccg ggttttgact ttcagcaagc
    19441 aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg gagcttgatc aatacatcaa
    19501 tcagaatttc acgcttgctt gtgaaaagat gttctggtgt aaagggaaag ttgtcgtcat
    19561 ggggatgggg aaatcggggc acatcgggcg caaaatggcg gcaacgtttg ccagcaccgg
    19621 tacaccttca tttttcgtcc atcctggtga agccgcgcat ggtgatttag gcatggttac
    19681 cccacaggat gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa tcacggcctt
    19741 aattccagtg cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc gcccggagag
    19801 cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag aagcctgtcc
    19861 gttagggctg gcaccgacca gcagcaccac cgccacgctg gttatgggcg atgccctcgc
    19921 tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat tttgcgctct cacacccagg
    19981 cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata cgggcgatga
    20041 gatcccgcat gttaagaaaa cggccagtct gcgtgacgca ttgctggaag ttacccgcaa
    20101 aaatcttggt atgactgtca tttgcgatga caatatgatg attgaaggca tctttaccga
    20161 cggtgattta cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa gtattgccga
    20221 tgtgatgacg ccggggggaa tacgtgtgcg ccctggcatt ctggccgttg aggcactgaa
    20281 cttaatgcag tcccgccata tcacctccgt gatggttgcc gatggcgacc atttactcgg
    20341 tgtgttacat atgcatgatt tactgcgtgc aggcgtagtg taaagattca aggataaaca
    20401 acaatgagca aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag cgccgacgtt
    20461 atagcaaaag cagagaacat tcgtctgctg atcctcgatg tcgatggcgt actgtcagat
    20521 ggcctgattt atatgggcaa taatggcgaa gagctgaaag cgttcaatgt tcgtgacggt
    20581 tatggcattc gttgtgcgct cacctctgat attgaagtcg ctatcattac cgggcgaaag
    20641 gctaaactgg tagaagatcg ttgtgccaca ttggggatca ctcacttgta tcaggggcag
    20701 tcaaataaac tgatcgcctt tagcgatctg ctggaaaaat tggcgattgc cccggaaaat
    20761 gtggcttatg tcggcgatga tctcatcgac tggccggtaa tggaaaaagt gggtttaagc
    20821 gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg ccgattacgt gacgcgcatt
    20881 gctggcggtc gtggcgcagt gcgcgaagtt tgcgacttat tactcctggc gcagggcaaa
    20941 ctggatgaag ccaaagggca atcgatatga gtaaagccag acgttgggtt atcattgtgc
    21001 tatcactggc ggttctggtg atgatcggca ttaatatggc cgaaaaagac gataccgccc
    21061 aggtggtcgt caacaacaat gatcccacct ataaaagcga gcatacggac acgctcgtct
    21121 ataacccaga aggggcacta agctatcgat tgattgctca acacgttgaa tattattccg
    21181 atcaggccgt ttcgtggttt acgcagccgg tacttaccac gtttgataag gataaaatcc
    21241 cgacatggtc cgtaaaagca gataaagcca agctgaccaa tgaccggatg ctctatttat
    21301 atggacacgt tgaagtcaac gcacttgtgc cagactctca acttcgcaga atcacgacgg
    21361 ataacgcgca gatcaatctg gtgacgcagg atgttacctc tgaagacctc gtcacgttat
    21421 acggaacaac atttaactcc agcggtctga aaatgcgcgg caacttacgc agcaagaacg
    21481 ccgagctgat tgaaaaggtt agaacatcct atgaaattca aaacaaacaa actcagcctt
    21541 aatcttgtgc ttgccagctc acttctggcc gccagcattc cggcatttgc cgtaaccgga
    21601 gacactgatc agccgatcca cattgaatcg gaccagcaat ctcttgatat gcaaggcaac
    21661 gtggttacct ttaccggtaa tgtcatcgtc acccagggca ccatcaaaat taatgccgac
    21721 aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag aagtgattga cggctacggt
    21781 aaaccggcaa cgttctacca gatgcaggac aacggtaaac cggttgaagg tcacgcttcc
    21841 cagatgcact acgaactggc aaaagatttt gtcgttctga cgggtaatgc ttatctgcag
    21901 caggttgata gcaacattaa gggcgataag atcacttacc tggtaaaaga gcagaaaatg
    21961 caggctttca gcgacaaagg caagcgcgta accaccgttc tggtgccgtc gcagctgcag
    22021 gacaaaaaca acaaaggcca gaccccggca cagaagaagg gtaattaatt cgttatggca
    22081 acattaactg caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt agaagacgtc
    22141 agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg tgccggtaag
    22201 accaccactt tctacatggt tgtaggcatt gtgccgcgcg atgcgggcaa catcattatt
    22261 gatgatgacg atatcagtct gctgcctctg catgcacgcg cgcgccgcgg tatcggctat
    22321 ctgccacagg aagcctccat tttccgtcgc ctcagcgttt acgataacct gatggcggta
    22381 ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa cgagctgatg
    22441 gaagagtttc acattgagca cctgcgtgac agcatggggc agtcactctc cgggggtgaa
    22501 cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat tctgctcgac
    22561 gaaccgtttg ccggggttga cccgatctcg gttatcgaca ttaaacgcat cattgagcac
    22621 ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca acgtgcgtga aacactggcg
    22681 gtttgtgaac gcgcttatat cgtcagtcag gggcatttga tcgcccacgg cacgcctaca
    22741 gaaatcttac aagacgaaca cgttaagcgt gtataccttg gggaagactt cagactctga
    22801 tagggtagaa gtttgcgacg ttttagcagg agagtacgat tctgaacatg aagcaaggtt
    22861 tgcaactcag gcttagccaa caactggcga tgacgccaca gctccaacag gcaattcgtc
    22921 tgttgcagtt gtcgacgctg gaacttcagc aggagctaca gcaggcgctg gagagtaatc
    22981 cgctgcttga gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg caagacagtg
    23041 aaacgctgga caccgccgac gcgctcgaac aaaaagagat gccggaagag ctgccgctcg
    23101 atgccagttg ggacaccatt tacaccgctg gtacaccatc cggcaccagc ggtgactaca
    23161 ttgacgatga gctgccggtc tatcagggcg aaacgacgca gaccttgcag gattacctga
    23221 tgtggcaggt cgagctgaca ccgttttccg acactgaccg cgctattgct acctctatcg
    23281 tcgatgccgt tgatgacacc ggttatctga ctgtcccgct ggaagatatt ctcgaaagta
    23341 tgggcgatga agagatcgac atcgacgagg ttgaagccgt ccttaagcgg atccaacggt
    23401 ttgatccggt cggtgtagcg gcaaaagatc tgcgtgactg cctgctaatc caactctccc
    23461 aattcgataa aaccacgccg tggctggaag aggccagact gatcattagc gatcatctcg
    23521 atctgttagc caatcacgac ttccgcactt taatgcgcgt cacgcgtctg aaagaagatg
    23581 tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc gcgccccggg cagtcgatcc
    23641 agactggcga acctgagtat gtcattccag atgtgctggt gcgtaagcat aacggtcact
    23701 ggacggtaga actcaacagt gacagcattc cgcgtctgca aatcaaccag cactacgcct
    23761 cgatgtgcaa taacgcgcgc aacgatggtg acagccagtt tatccgcagc aatctgcagg
    23821 atgccaaatg gttgatcaag agtctggaaa gccgtaacga tacgctactg cgcgtgagtc
    23881 gctgtatcgt tgaacagcag caagccttct ttgagcaagg tgaagaatat atgaaaccga
    23941 tggtactggc cgatatcgcc caggctgtcg aaatgcatga atcgacgata tctcgcgtga
    24001 ccacgcaaaa atacctgcat agtccacgag gcatttttga actgaagtat ttcttttcca
    24061 gtcacgtcaa taccgagggc ggcggcgaag cttcctccac ggcgattcgt gcgctggtga
    24121 agaaattaat cgcggcggaa aacccagcga aaccgttgag cgacagcaag ttaacctctt
    24181 tgctgtcgga acaaggtatc atggtggcac gccgcactgt tgcgaagtac cgagagtctt
    24241 tatccattcc gccgtcaaac cagcgtaaac agctcgtttg acccaaccga taaggaagac
    24301 actatgcagc tcaacattac cggaaataac gtcgagatca ccgaggcact gcgcgaattt
    24361 gttacagcca aatttgccaa acttgagcaa tattttgacc gaatcaacca ggtctatgtt
    24421 gttctgaaag tggagaaagt cacccacacc tcagatgcaa cactgcatgt aaacggtggt
    24481 gaaattcatg ccagcgcgga aggtcaggac atgtacgctg ccattgatgg cttaattgat
    24541 aagctggcac gccagttgac caaacataaa gataaactga aacaacacta attgtccggg
    24601 caattagcat gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt acggttaatg
    24661 ctccgagcct gttccactgt ttgagtgggc aggttcttag gtgaaattat gacaaataat
    24721 gatacaactc tacagcttag cagtgttctt aacagggaat gtacgcgaag ccgcgtccac
    24781 tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa acaacttagc
    24841 ctgccgcctc aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg cagtaccggt
    24901 atcggcaatg gtattgcaat tccgcatggc aaactggaag aagatactct gcgtgccgtt
    24961 ggcgttttcg ttcagctcga aacgcctata gctttcgatg ccatcgacaa ccaaccggtg
    25021 gacttacttt ttgccctgct ggtgccggca gaccaaacta aaacgcacct gcatactctg
    25081 tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc gccgtttacg cgcagcccag
    25141 agcgatgaag agctgtatca aatcattacg gataccgaag gtactccgga tgaagcgtag
    25201 ttattcggta atgtctcttt tagacgttgt gaggagaaac agtacatggt actgatgatc
    25261 gtcagcggac gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga agatatgggt
    25321 ttttactgcg tggataacct tcccgtagtg ttgttacccg atctggctcg aactctggcc
    25381 gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta atatgccgga gtcaccagaa
    25441 atattcgaac aggcgatgag taacctgcct gacgctttct caccgcaact actgttcctg
    25501 gatgccgacc gtaatacctt aattcgtcgt tacagtgaca cgcgccgact gcatccgctt
    25561 tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag aaagcgattt gctggagcct
    25621 ctgcgttcgc gagcggatct gattgtcgat acctcagaaa tgtccgttca cgagctggct
    25681 gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg aactgaccat ggtctttgag
    25741 tctttcggct tcaaacacgg tatccctatc gatgcagatt acgtctttga cgtgcgcttc
    25801 ttgccgaacc cgcactggga tccgaaactg cgtccaatga caggtcttga taaacctgtc
    25861 gccgcgttcc tcgaccgcca cacagaagta cacaatttta tctaccagac gcgaagctat
    25921 cttgaactat ggttacctat gctggaaacc aacaaccgta gctacttgac ggtcgccatt
    25981 ggttgtaccg gcgggaagca ccgttcggtg tatattgcag agcaactggc agactacttc
    26041 cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg taaaccatga
    26101 ccgtcaagca aactgttgaa atcacaaaca agctgggcat gcatgcccgg cctgcaatga
    26161 agctgtttga attaatgcag ggttttgacg ctgaagtgct gttacgtaat gacgaaggca
    26221 ccgaggccga agccaacagc gttattgcgc tgctgatgtt ggattctgcc aaaggacggc
    26281 agattgaagt tgaagcgacc ggcccacagg aagaggaagc actggccgcc gttatcgccc
    26341 tctttaactc tggttttgat gaagattaat cttcatcact ttgacataca actcccttca
    26401 aactcccccc gacaataaga aaatcacgta ctgaaatcgt tctcaatcaa cgtcatttgt
    26461 acattttgtg cgcttttcac tttcagaaga accttaagaa aaccttaaga ggcattgttt
    26521 aggttttgtt taagttaatc gaccatactg gagatcgtca gaaaatattt ccaggagatg
    26581 gcatgattcg cttatctgaa caaagtcccc tgggcaccgg aagacatcgg aaatgttatg
    26641 cgcatccgga agatgcccaa cgctgtatca agattgtcta ccatcgtggc gatggtggcg
    26701 ataaagagat ccgccgtgag ttaaagtact acgcgcatct tggtcgccgg ttaaaagact
    26761 ggagtggaat accgcgctat cacggtaccg ttgagacgga ttgcggaacc ggttatgtct
    26821 acgatgtgat tgcagatttt gacggcaaac cctccattac gctgaccgaa tttgctgaac
    26881 aatgccgtta cgaagaagac attgcacaac tgcgccagct actgaaacag ctgaagcgtt
    26941 atttgcagga taaccgtatc gtgacgatgt cgctgaagcc gcagaatatc ctctgtcacc
    27001 gcatcagtga gtcagaggtt atcccggtcg tctgtgacaa cattggtgaa agcacattga
    27061 ttccactggc gacctggtcg aagtggtgtt gtctgcgtaa gcaagagcgg ttatggaaac
    27121 gatttatcgc ccaaccggcg ctggctattg ccctgcagaa agatctgcaa cctcgtgaga
    27181 gcaaaacgct ggccctcact tcgcgcgaag cttaatccag ctggtgttgt tgcataaacg
    27241 gctcaccgcc taactgatac atctgccgta aaatccacgc ctgacggcta cgcacgtagc
    27301 ccgatggcga ggagactttg aaacgaagtg gattaggtaa tacagctgcc agtaatgcag
    27361 cttccgaccg ggtaagtttg ctcgcgggtt tgtggaaata acgttgtgcc gcagcttcga
    27421 cgccaaacac accgtcgcca aattcggcga tattcaggta aaccgtcagg atacgctttt
    27481 tgctccagac tgtttctatc cccagcgtta atccagcttc cagccctttt cgtacccagc
    27541 tacgcccatc ccataaaaag agatttttgg ccgtctgctg agaaatcgtt gaagcaccgc
    27601 gaatacggtt ttcattgcgc tcgttgtgcg ccagggcttt ctcaatggaa gcgacatcaa
    27661 agccccagtg ctcaggaaat ttctgatctt ctgcggcaat caccgccagt cccatccacg
    27721 gcgagatttg atccatactg acccagtcag aatgtgccac gtaacgaaaa ttgccatgca
    27781 gccaggcgct gacctgtcgc tcgaccatta ccgctgagaa gggaacaggc gcaacactaa
    27841 acaacgcgat gcccccgccc cagaaaacgg cgaggacaac cattaaccgc aaaagaaaac
    27901 ggcgaacgaa actaaacacc gttaagcggc ttttactcat tcagccagaa ccagcacgcg
    27961 ggaaaccagc ttatcaatgc cgctcgccgc ttctgcaatg ttctgcgcca gcatatatgc
    28021 tggggtggtg acaatcttat tgtcttcatc aaccacgata tcatcgacag gacagggcac
    28081 atgctccgcg cccatctctt ccagcacttc tgcggtatcg atatcagtac cgatggtcaa
    28141 acgcagcggg aaatcgaaaa ttttcggcag catcgccggg gcaatacaca taaaaccaag
    28201 cggttttccg gcctgatgca tcgcttgtgc cagcgccttt aattcacggt caacggtgca
    28261 ttcgctacca agagtggcaa aattgcttaa attcttcgcc gcgccaaacc cccccggcac
    28321 aatcaacgca tccagttcag cggcatcggc ctgggccaga ggacggattt caccgcgcgt
    28381 tattcgtgcc gcttcaatca gcacgttgcg cgtttccgtc atcgcttcgc cagttaaatg
    28441 gttgataaca tcaacctgct gcttatccgg tgcaaagcag accgcctgcg caccgctgcg
    28501 tgaaatagct aacaacgtca acaccgcttc atgaatttca gaaccgtcat agacgccgca
    28561 tccgctcaga attacgccaa ttttcttcat tgtgatcatc ctttcgcaat tgactgaaac
    28621 acattaattt ttttaataaa aatggtacgc atcacacatt taactgattc atgtaacaaa
    28681 tcatttaagt tttgctatct taactgcgtg cggcctgaaa aacagtgctg tgcccttgta
    28741 actcatcata ataatttacg gcgcagccaa gatttccctg gtgttggcgc agtattcgcg
    28801 caccccggtc tagccggggt cattttttag tggcttttgc cacccacgct ttcagcactt
    28861 ctacgtcgtg acgccactct tctttcatct cttcaatcca ttcaccgacg ttatcttccc
    28921 aggcaggaag gtccggagac tgaatttgct gacccagctg ttgcagatgg cgtaacccca
    28981 ctgaccccgc cgcaccttta attttatgtc cttcctctac aatgcctttt ttatcctgcg
    29041 ccgtcagatt cgactccagc acgctgacat agcccggcat catcttctca aacaccgcta
    29101 acccgtcggt gatcagcttc ggtcctacaa gttcgagata ctgttccagc atgggaatat
    29161 cgagcaatgc ttctgattta ctgttctctt ctgtcgtcac cgtactctcc tcatcatcct
    29221 gggtatccca gaatttcttg atcatcgcgg ttagcgccgg aaccgaaagc ggcttactca
    29281 gcacatcatc cattccagca ttgaggtact cttgtttgtc tttcagcacg ttagcggtta
    29341 aggccaccag cggcggtaaa tcctcgcgcg gataacgctt cgtcagttca cgagagatat
    29401 ccagcccggt catatctggc aactgaatat ccagcaacac caggtcgtat tcgcccggtt
    29461 taaacatctc cagcgccgcc ttgccggtca tggcgacatc aacgctgtta cctaattttt
    29521 ccagcacaga acgcgcaaca atcacgttca gttcaatgtc ttccaccagc agcacattca
    29581 gcgccggtaa aggcatatcg tcttcatcaa acgcatcatc gacctcttct gctaccgacg
    29641 gtgcgtggat cgtcaacgta aaggttgaac ctttgccctg ttcgctggta accgtaatat
    29701 cgccgcccat atttttcgcc agacgacgag aaacggccag accaataccg gtgccggtgg
    29761 caggtttacc gccatgactg tctttcacct ggtaatacat ggcgaaaatt ttatccagct
    29821 catcctgcgg aatgccgata ccagagtctt ccacttcaaa atgcagcata tcgccttcat
    29881 cgtagcgcac gcgcacggta acctggcctt gctgggtgaa tttgacggcg ttactgatga
    29941 ggttccacag gatctgccgt aaacgcgtcc cgtcggtaat gacctgatgc ggtaatggca
    30001 gcgtcggctc caggttaaag cgcaatcctt tttgttgcgc ctgcaatgcg gagagatttt
    30061 ccagatcggc aaggaagctg gtgaaatcaa ccggttgatt atcaagctgg accttgcgcc
    30121 gttccatctt atccatgtcg ataatatcgt taaagatatt ccccagcgtg acggccgaaa
    30181 catggatggt cttgagatat ttttcctgct cggcggtgag ttcggtatcc agcagaatac
    30241 ggctcaggcc gacgataccg ttcagcggtg tacgcaattc gtgactgatg gtggagataa
    30301 acgtcgtttt gtcgcggctg gcccgttcaa gcgcatcctg ataccgctta cgctcggtaa
    30361 tgtcgcgacc aaagcccatc aaaccgtgac gtttacccac gcggtcgtag tacggcactt
    30421 tacggatttc aaagcaggct ttgcgcccgt ccgggtaatc cagccactgt tcataggtca
    30481 gtgacacatt atgacggaac actttttcat cggtttcaat gacttttgcg gcggcttccg
    30541 gtgagtaaac atcagcaggt ttcaggtgaa ccagttgttt ttcgcttttt ccggtcagca
    30601 gctccatcgc gcggttacag ccggaaaact ctttatcttc gttacgataa aaaaccaggt
    30661 cgggtgaagc atcaaggaag gaacgtaaga atgaggattg ctgctcgagc tgaatttgtg
    30721 tctcttcgcg ctctttgatt tcaattttca gttggccgaa ggtttcctgc agttctgctt
    30781 ccgctttttc acgaacggcg atttcctgat ttagctgggc aatattatct tttaactgaa
    30841 cgttgaggct caaatcgcgc tcgcgcatct cctccagttt ttgcaccagc cgtgacagac
    30901 gttgtcgtga ctcctccagt tgctcgacga ccaccgatag aaagtagacc gcccacggcg
    30961 taatcagcaa accaaagaag atagaacgaa taacatcaat gctttcgacc tgaccatgca
    31021 gcaccatggt taccgccatt tgtaccacaa tggcaagaac gacgagggcc agcgccagca
    31081 acattgagaa gcgcaccaga cctaacttca tcatcaggtc aacataatac tgcgccagca
    31141 gacgaatttg cttcattagg gaattccttc acgacaacct gccccaataa tacccaattc
    31201 tgacagttac gttataaatt gtgtgagaaa tgcggaattc ccctcaacga cctactccgt
    31261 tggaggtatc cacggacgtt ccagcgccga tccctgtacg ccatgttcat tcagatagcg
    31321 atccagctcg accattcccg tccatcgatt ttcgcaccac agaggagcaa gcagcgtcgg
    31381 gcgacgggcg ctggcggaaa tgcggtggta gatcacttcc ggtggcgtat ggcgaatcat
    31441 ttctccggca gtgagcgtgt aatcctccag ttcaataccg ttcaaacgcc ccgcttccca
    31501 ggctttcgcc ataatgctgc ctttcacaat atgcagcgga tgcagcttta tgccgtccac
    31561 gccggtttca accactcgct ccagcgtttg caaacattca gcctgccctt cgccaggcag
    31621 gccgacaatt aagtgggaac acaccttcag cccgcgctga cgtgccagct gggttgtacg
    31681 ctgataacag gcaaaatcat ggccgcggtt gatgcgatgc agtgttttgt cgtgggcggt
    31741 ttgtagcccc agctccagcc acacttcgta accctggtcc ttatattcgc aaagcagatc
    31801 cagcaccgca tccggcacgc agtccgggcg ggtaccaaca cacaaaccga caatattggc
    31861 ctggctcacc gcctgctgat acatagaacg cagcacctga acttccgcaa aggtgctggt
    31921 atacgcctga aagtaggcca gatagcgttt agcgcggtta actaaattcg cctggtgcgc
    31981 cagttgctcg gcaatggaac gatgctgctg cgcttcatcg gcaaacgagg caacattaca
    32041 gaatgtgcag ccaccacgcc cgatggtacc gtcacggtta gggcagctaa aaccgccgtg
    32101 tagcgtcagc ttatgcacct tttgcccata acgacgggtg agatcaccac caaacatatt
    32161 gactaatttc tgtaactgca taatctgata gaccgcgcct tgaaaagagg ccaaagcctg
    32221 ccatttttag ctcaattcgg cgatgacctg gatcaatcgt cccggcccgc ttttatcaac
    32281 tgcataatca atcaaaatta ccgaaatttc atgcataatc acataaatca cttttgctta
    32341 tcttgtgtca gattttttta tctccttatg gattttaggc aaaaacagta gcatgaaacg
    32401 tcattaccaa ttaaggcagt ataaaatgct ggttttctcg tcagttcaag gcaggataag
    32461 ggttaacacg cctttatgac agtcaggaat tgactgtttc tctaacgact tcccttttag
    32521 ccttaaagat aaaatcaatt ttaatttcag taatttaata aagaattttg cgctaaagca
    32581 catttctgta ccaataagct tgccatttga cctgtatcag ctttcccgat aagttggaaa
    32641 tccgctggaa gctttctgga tgagcagcct gctcatcata tttatgcagt aattgagatc
    32701 ccctcttcac cgtattaacc gatgcgaaaa ggacaacaag ggggcgaatg cgaggcgcgc
    32761 gtatgacacg caaaccccgt cgccacgctc tttctgtgcc cgtgcgcagc ggttcggaag
    32821 tggggttccc gcagagcctg ggggaggttc acgatatgtt gtacgataaa tcccttgaga
    32881 gggataactg tggtttcggc ctgatcgccc acatagaagg cgaacctagc cacaaggtag
    32941 tgcgtactgc aatacacgca ctggcccgca tgcagcaccg tggcgcgatt ctcgccgatg
    33001 gtaaaaccgg cgacggttgc ggcttgctgt tacaaaaacc ggatcgcttt tttcgcatcg
    33061 ttgcgcagga gcgcggctgg cgtttagcaa aaaactacgc tgtcgggatg ctcttcctga
    33121 ataaagatcc tgaactcgcc gctgccgcac gccgcatcgt tgaagaagag ctgcaacgcg
    33181 aaaccttgtc gattgtgggc tggcgtgatg tccccactaa cgaaggcgtg ctgggtgaaa
    33241 tcgccctctc ctctctgcca cgcattgagc aaatttttgt gaacgccccg gcaggctggc
    33301 gtccgcgcga tatggagcgc cgtctgttta tcgcccgccg ccgcattgaa aagcgtctcg
    33361 aagccgacaa agacttctac gtctgtagcc tgtcgaatct ggtgaacatc tataaaggtc
    33421 tgtgtatgcc ggcggatctg ccgcgctttt atctggatct tgcggacctg cgtctggaat
    33481 cggccatctg cctgttccac cagcgcttct ccactaacac cgtaccgcgc tggccgctgg
    33541 cgcagccgtt ccgctatctg gcgcataacg gtgaaatcaa caccatcacc ggtaaccgcc
    33601 aatgggcgcg tgcgcgtacc tataaattcc agacgccgct tatccctgat ctgcacgacg
    33661 cagcgccatt cgtcaacgaa accggttctg actccagctc gatggataac atgctggaac
    33721 tgctgctggc aggcgggatg gatatcatcc gcgctatgcg tctgttagtg ccgcccgcct
    33781 ggcagaacaa cccggatatg gacccggagc tgcgtgcctt cttcgacttt aactccatgc
    33841 atatggagcc gtgggatggc ccggcgggca tcgtgatgtc cgacggtcgt tttgccgcct
    33901 gtaacctcga ccgtaacggt ctgcgtccgg cgcgctacgt catcaccaaa gataagctca
    33961 tcacctgcgc ctctgaagtc ggtatctggg attatcagcc tgacgaagtg gtcgaaaaag
    34021 gccgcgtcgg gccaggcgaa ctgatggtga tcgacacccg cagtgggcgt attctgcact
    34081 cggcagaaac cgatgacgat ctgaaaagcc gccatccata taaagagtgg atggagaaaa
    34141 acgtccgccg actggtaccg tttgaagatc tgcccgatga agaagtgggt agccgcgaac
    34201 tggacgacga cacgcttgcc agctaccaga aacagtttaa ctacagcgcg gaagagctgg
    34261 actccgtaat tcgcgtactg ggcgaaaacg gtcaggaagc ggtcggttcg atgggcgatg
    34321 ataccccatt cgccgtgctc tccagtcagc cgcgcattat ttacgactac ttccgccagc
    34381 agtttgccca ggtgactaac ccgccaatcg acccgctgcg tgaagcgcat gttatgtcgc
    34441 tcgccaccag tatcggtcgt gaaatgaacg tcttttgcga agcagagggc caggcgcacc
    34501 gtttaagctt taaatcgccg attctgctct actccgattt caaacagctc acgacgatga
    34561 aagaggagca ctaccgcgca gatacgctgg atatcacctt tgacgtcact aaaaccacgc
    34621 tcgaagcgac agtcaaagag ctgtgcgaca aagccgaaaa aatggtacgt agcggcaccg
    34681 tgctgctggt gctctccgac cggaatatcg ctaaagatcg cctgccggtt ccagccccga
    34741 tggcggttgg cgcgatccag acccgtctgg tcgatcaaag cctgcgttgc gatgccaaca
    34801 tcatcgtcga aaccgccagc gcccgcgatc cgcaccactt cgccgtgttg ctgggcttcg
    34861 gcgcgacggc tatttatcca taccttgcct atgaaacgct gggccgcctg gtagacaccc
    34921 atgcgattgc caaagattat cgtaccgtga tgctcaacta ccgtaacggc atcaacaaag
    34981 gcttgtacaa aatcatgtcc aaaatgggca tctccaccat cgcctcttac cgctgctcga
    35041 aactgtttga agcggtcggt ctgcacgatg atgtagtggg cctgtgcttc cagggggcgg
    35101 tcagccgcat tggtggggcg agctttgaag acttccagca ggatctgctg aacctgtcga
    35161 aacgtgcctg gctggcgcgt aagcccatca gccagggcgg cctgctgaaa tacgtccacg
    35221 gcggcgaata ccacgcttac aacccggacg tggtgcgcac gctgcaacag gcggtacaaa
    35281 gcggcgagta cagcgactat caggaatacg cgaagctggt taatgagcgt ccggcaacca
    35341 cgctgcgcga tctgctggca attacgccgg gtgaaaacgc ggtcaacatt gctgatgttg
    35401 aaccggcaag cgaactgttt aaacgctttg ataccgccgc gatgtctatc ggcgcgttaa
    35461 gcccggaagc ccacgaggcg ctggcggaag cgatgaatag catcggcggt aactcgaact
    35521 ccggtgaagg cggcgaagac ccggcgcgct atggcactaa caaagtgtcg cgcatcaagc
    35581 aggtggcttc tggtcgcttc ggcgttactc cggcgtatct ggtcaatgcc gacgtcattc
    35641 agattaaagt cgcccagggc gcgaagccgg gcgaaggcgg tcagttgccg ggcgacaaag
    35701 tcactcctta catcgccaaa ctgcgctatt cggtgcccgg agtgacgctg atctccccgc
    35761 cgccgcacca cgatatctac tctatcgagg acttagcgca gctcattttc gacctcaagc
    35821 aggttaaccc gaaagcgatg atctccgtga agctggtttc cgaaccgggc gtaggcacca
    35881 tcgcgactgg cgtggcaaaa gcttatgccg atttaatcac catcgcaggc tatgacggtg
    35941 gcaccggcgc aagcccgctg tcgtcagtga aatacgcagg ttgcccgtgg gagctggggc
    36001 ttgttgaaac ccagcaggcg ctggttgcca atggtctgcg tcataagatc cgtttgcagg
    36061 tcgatggcgg cctgaaaacc ggtgttgata tcatcaaagc ggcgattctc ggcgcagaaa
    36121 gcttcggctt cggcactggc ccgatggtgg cgctcggctg taaatatcta cgtatttgcc
    36181 atctgaacaa ctgcgcaacg ggtgtagcaa ctcaggatga caaactgcgt aagaaccact
    36241 atcacggcct gccgttcaag gttaccaatt actttgaatt tattgcccgt gaaacccgcg
    36301 agctgatggc acagctgggc gtaacgcgtc tggtggatct gattggtcgc accgacctgc
    36361 tgaaagaact ggacggtttc accgccaaac agcaaaaact ggcgctgtcg aagctgctgg
    36421 agaccgccga accgcatcca ggtaaggcac tctactgcac cgaaaacaac ccaccgtttg
    36481 ataacggcct gctgaacgcg cagttgctgc aacaggcgaa accgtttgtc gatgaacgcc
    36541 agagcaaaac cttctggttc gatattcgca acaccgaccg ttctgtcggc gcgtcgcttt
    36601 caggctatat cgcccagacg cacggcgatc aggggctggc agccgatcct atcaaagcgt
    36661 acttcaacgg caccgcaggc cagagtttcg gtgtgtggaa cgcgggcggc gtggaactgt
    36721 acctgaccgg ggatgccaac gactatgtcg gtaaaggcat ggcgggcggc ttaatcgcca
    36781 ttcgtcctcc ggttggttcc gccttccgca gccatgaagc aagtattatc ggcaacacct
    36841 gcctgtatgg cgcgaccggt ggtcgtctgt atgccgcagg ccgcgcgggt gaacgtttcg
    36901 gcgtgcggaa ctccggtgct atcaccgtgg tagaaggcat tggcgacaac ggctgtgaat
    36961 atatgacggg cggtattgtc tgtattctgg gtaaaaccgg cgttaacttc ggtgcgggca
    37021 tgaccggcgg cttcgcttac gttctcgatg aaagcggtga tttccgcaaa cgcgttaacc
    37081 cggaactggt cgaggtctta agcgttgacg atctggcgat ccatgaagag catctgcgcg
    37141 gtcttatcac cgagcatgtt cagcatactg gctctcagcg cggtgaagag attctggcga
    37201 actggtcaac cttcgccact aaatttgcgc tggttaaacc gaagtccagt gatgtaaaag
    37261 cactgctggg tcatcgtagt cgtagcgcag cagagttgcg cgtgcaggcg cagtaagggg
    37321 tagcaacaat gagtcagaat gtttatcaat ttatcgacct gcagcgcgtt gatccgccaa
    37381 agaaaccgct gaagatccgc aaaattgagt ttgttgaaat ttacgagccg ttttccgaag
    37441 gccaggccaa agcgcaggct gaccgctgcc tgtcgtgcgg caacccatac tgcgagtgga
    37501 aatgtccggt acacaactac atcccgaact ggctgaagct cgccaacgag gggcgtattt
    37561 ttgaagcggc ggaactgtcg caccagacca acaccctgcc ggaagtttgc ggacgagtct
    37621 gcccgcaaga ccgtctgtgc gaaggttcct gcactctgaa cgatgagttt ggcgcggtga
    37681 ccatcggcaa cattgagcgt tatatcaatg ataaagcgtt cgaaatgggc tggcgtccgg
    37741 atatgtccgg cgtgaagcaa accggtaaaa aagtggcgat tatcggcgca ggcccggcag
    37801 gtctggcgtg tgcggatgtc ctgacgcgta acggcgtaaa agccgttgtc ttcgaccgtc
    37861 atccagaaat tggcgggctg ctgaccttcg gtattccggc cttcaagctg gaaaaagagg
    37921 taatgacgcg tcgccgtgaa atcttcaccg gcatgggtat tgaattcaaa ctcaataccg
    37981 aagtgggccg cgacgtgcag ctggacgatc tgctgagtga ttacgatgcc gtcttccttg
    38041 gcgtcgggac ttatcagtca atgcgcggcg ggctggaaaa cgaagacgcc gatggcgtgt
    38101 acgcagcgct gccgttcctt atcgccaaca ccaaacagtt aatgggcttt ggtgaaaccc
    38161 gcgacgaacc gttcgtcagc atggaaggca aacgcgtggt ggtccttggc ggtggcgaca
    38221 ctgcgatgga ctgcgtgcgt acgtccgtgc gccagggagc gaagcacgtt acctgtgcct
    38281 atcgtcgtga tgaagagaac atgccgggtt cccgccgcga agtgaaaaac gcgcgggaag
    38341 aaggcgtaga gttcaaattc aacgtccagc cactggggat tgaagtgaac ggtaacggta
    38401 aagtcagcgg cgtaaaaatg gtacgtaccg aaatgggcga accggatgcc aaaggccgtc
    38461 gccgcgcgga gatcgttgca ggttccgaac atatcgttcc ggcagatgca gtgatcatgg
    38521 cgtttggttt ccgtccacac aacatggaat ggctggcaaa acacagcgtc gagctggatt
    38581 cacaaggccg catcatcgcc ccggaaggca gcgacaacgc cttccagacc agcaacccga
    38641 aaatctttgc tggcggcgat atcgtccgtg gttccgatct ggtggtaacc gctattgccg
    38701 aaggtcgtaa ggcggcagac ggtattatga actggctgga agtttaagct ctgtcccagc
    38761 gccgcctcct cagaaaagag tcggcgctga aattctcctt tctttctgga aagcgcctcg
    38821 cataatctga agaaaaccaa tgtaatacgc tcatctggat tgttaccggt agatgcccgg
    38881 cgtatagctg attccattat tcaacgcagc gccatataaa gggacagata atggaatcgc
    38941 tctcagagag aacctcaaca ggctaccagc aaatccacga cggtattatt catctggtcg
    39001 atagcgcccg gacggaaacg gtacgtagcg ttaacgcgct gatgaccgcg acatactggg
    39061 aaattggccg acgaattgtc gaatttgaac aaggtggcga ggccagggct gcgtatggtg
    39121 cgcagctaat caagcgacta tcaaaggatt taagtctaag gtataagcgt gggttctctg
    39181 caaaaaactt acgccaaatg aggctttttt acctcttttt tcaacatgtt gaaattcgcc
    39241 agacagtgtc tggcgaatta acaccattgg gaattccgca gacaccatct gcagaattcc
    39301 catcggcaaa gatttggcag acgctgtctg ccaaattgag aggctgtttg atatttccac
    39361 agcttttgta ttgcatctcc tattttcggt ttgtatattc atgcaaataa acgaaggaaa
    39421 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
    39481 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
    39541 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
    39601 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
    39661 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
    39721 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
    39781 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
    39841 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
    39901 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
    39961 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
    40021 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
    40081 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
    40141 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
    40201 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
    40261 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
    40321 tggcggtagt gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
    40381 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
    40441 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagaga
    40501 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctagca
    40561 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcaattgct
    40621 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
    40681 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
    40741 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
    40801 tgatactccg cccatgctgc agcctgcgcg tcgttaatgg caatggtcgg caaattggta
    40861 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
    40921 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
    40981 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
    41041 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
    41101 gcggcaagtt tagtaccgcc gatatcaatc gccagcgtgg tcatagcacc gcctttttca
    41161 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
    41221 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
    41281 cggcaatcac ccgacatccg gcgtcgctca acgttttcac cagcgccaga tccggctctt
    41341 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
    41401 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
    41461 ggtgaatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
    41521 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
    41581 tgcgtaccgg agaatcctcc agatcgcgtt tcacaatccc gataattggc acgctcacca
    41641 ccgcacgcgt ggcttgcaga tttgccacac cttcaatgcg aatggcaacc gcgcccgcct
    41701 gctctgccgc taatgccatg gcggcgacga tttcgggttt atcgagcgga ctgtccggaa
    41761 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
    41821 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
    41881 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
    41941 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
    42001 ggaaggcata tccagaccaa tcagcaggat caccacgaac gtcagactga acgagagcga
    42061 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
    42121 tgcgccgccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
    42181 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
    42241 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
    42301 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgcggg ttcccagcca
    42361 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
    42421 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
    42481 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
    42541 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
    42601 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
    42661 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
    42721 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
    42781 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
    42841 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
    42901 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
    42961 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
    43021 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
    43081 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
    43141 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
    43201 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
    43261 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgccc gccattgtgc
    43321 acgattgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
    43381 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
    43441 cccctcaccc ggtaggggcg agcgagggga aacaactcac ccgcgctctt gcatcaactg
    43501 ctgggccagc gccttcagtt ctggcagata tttttcatct accggaccaa acggtttgcg
    43561 gcacagcggc acagaaacga catccatata atgcaggaca gttttcaggc cgcggaatac
    43621 gcccgttttg atcagtaaat caatgacttt attacattcg gtttgcagct tctgcgcggt
    43681 ctggatatcg ccttctttca gcgccttcac aatcccctga taacgccagc ccatgatgtt
    43741 gtaggtacta ccgataccac catcagcgcc cgccagaaga ccagaggcga agatttcgtc
    43801 gtaaccgtta tagagcacca gatcaggatg ttcacgacgg atctgctcca tctgatagag
    43861 atcgccagag gtctgtttca gcgcacctac gccaggcaac gtaacaagtg tgttgatctg
    43921 atccagggtc agttttaccc cactcagggc tggaatgttg tacaccacca tcggcaaacc
    43981 atccgccgaa tcaataattg cccgatagtg atcgcagtgt tcttcaaagc tgaaaggata
    44041 gtagaacggc gtgacggcgg agacggcatc gaagccataa cgtttagccg atgccgcaag
    44101 ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg atgagtttaa tcttaccttt
    44161 cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg gaaaggcttt gtacaaaggc
    44221 ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc tgctgaatat tgaactgaac
    44281 caggcgacgc agactcgctt tatccagtgc ttgttgttgg tcaaaaggag tcaggagtgc
    44341 agccattacg ccacgtaaat tcgttgccat aaatacctct gaagtgatgc ttgtctgata
    44401 aacgatatac ctttatacct gttataccag atcaattaag caacacccca tacagaaagc
    44461 ttataatgcg atctgcttca ctaaagtggc attatttctt tttgttggtg gtctgaccga
    44521 aagcgtgcca ggtagcagag acgctgttga gatgcgattg caacgcacga tcggcttcgt
    44581 caggatcgtg gcggcggatc gcgtcaacga tcgcaatatg ctgttgatag ctaacgttgt
    44641 tatgctcgtg cagagcctga tcagccacgg ttggacgtgc ggcgattagc cagtcgagca
    44701 gggcaacgtg gatcgccatg aagattgggt tacctgggat ctccgccagc acgcggtgga
    44761 aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag cgactgactg ttgatttcca
    44821 gtgcttttgc cagcaaatcg atttgctcat cggtggcgtg ttcagccgca tagcgcacca
    44881 ggctggattc aaagaacaga cgtaattgtt cgaaatgggc aatcccacca ggatgagaaa
    44941 ggaaatcttt cgccatgccg gaaagctcac cgatgatagt gtccgcagaa ggacgcgaga
    45001 cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt gcgttttaac gctgccagcg
    45061 cttcgcgcac agaaggacgc ccgacgttaa agaacgccat cagttcgcgt tcagacggta
    45121 attgttcacc ttcgccaaat tcacgacggc ggatcatctg ttccagctct tcttccacca
    45181 tttcggagag ttttttacgc gccagcgggc ggctacgcaa gttgcgacca attgcaggtg
    45241 aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc cattctgtaa ggtcagtgtg
    45301 attaacatca tcagtgacat cctatcacag gattgaaagt aggggaaaat ggcaggtttt
    45361 tctctttgtg cctcatcatt accataatta acggaataat taactattgc gaaaaattaa
    45421 t
//
LOCUS       DAPGWD010000022        44526 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPGWD010000022 DAPGWD010000000
VERSION     DAPGWD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.022"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(179..1543)
                     /gene="mnmE"
                     /locus_tag="RHX88_001151"
     CDS             complement(179..1543)
                     /gene="mnmE"
                     /locus_tag="RHX88_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDV2229874.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1649..3295)
                     /gene="yidC"
                     /locus_tag="RHX88_001152"
     CDS             complement(1649..3295)
                     /gene="yidC"
                     /locus_tag="RHX88_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDV2229875.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3298..3555)
                     /gene="yidD"
                     /locus_tag="RHX88_001153"
     CDS             complement(3298..3555)
                     /gene="yidD"
                     /locus_tag="RHX88_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDV2229876.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3519..3878)
                     /gene="rnpA"
                     /locus_tag="RHX88_001154"
     CDS             complement(3519..3878)
                     /gene="rnpA"
                     /locus_tag="RHX88_001154"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDV2229877.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3895..4035)
                     /gene="rpmH"
                     /locus_tag="RHX88_001155"
     CDS             complement(3895..4035)
                     /gene="rpmH"
                     /locus_tag="RHX88_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDV2229878.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4265..4345)
                     /locus_tag="RHX88_001156"
     CDS             complement(4265..4345)
                     /locus_tag="RHX88_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229879.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4642..6045
                     /gene="dnaA"
                     /locus_tag="RHX88_001157"
     CDS             4642..6045
                     /gene="dnaA"
                     /locus_tag="RHX88_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDV2229880.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6050..7150
                     /gene="dnaN"
                     /locus_tag="RHX88_001158"
     CDS             6050..7150
                     /gene="dnaN"
                     /locus_tag="RHX88_001158"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDV2229881.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7150..8223
                     /gene="recF"
                     /locus_tag="RHX88_001159"
     CDS             7150..8223
                     /gene="recF"
                     /locus_tag="RHX88_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDV2229882.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8252..10666
                     /gene="gyrB"
                     /locus_tag="RHX88_001160"
     CDS             8252..10666
                     /gene="gyrB"
                     /locus_tag="RHX88_001160"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDV2229883.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10906..11304
                     /locus_tag="RHX88_001161"
     CDS             10906..11304
                     /locus_tag="RHX88_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDV2229884.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11419..12231
                     /gene="yidA"
                     /locus_tag="RHX88_001162"
     CDS             11419..12231
                     /gene="yidA"
                     /locus_tag="RHX88_001162"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDV2229885.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12277..12933)
                     /locus_tag="RHX88_001163"
     CDS             complement(12277..12933)
                     /locus_tag="RHX88_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229886.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGANDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13211..13900
                     /gene="dgoR"
                     /locus_tag="RHX88_001164"
     CDS             13211..13900
                     /gene="dgoR"
                     /locus_tag="RHX88_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDV2229887.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13897..14775
                     /gene="dgoK"
                     /locus_tag="RHX88_001165"
     CDS             13897..14775
                     /gene="dgoK"
                     /locus_tag="RHX88_001165"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDV2229888.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14759..15376
                     /gene="dgoA"
                     /locus_tag="RHX88_001166"
     CDS             14759..15376
                     /gene="dgoA"
                     /locus_tag="RHX88_001166"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDV2229889.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15373..16521
                     /gene="dgoD"
                     /locus_tag="RHX88_001167"
     CDS             15373..16521
                     /gene="dgoD"
                     /locus_tag="RHX88_001167"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDV2229890.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16596..17933
                     /locus_tag="RHX88_001168"
     CDS             16596..17933
                     /locus_tag="RHX88_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2229891.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17930..18994)
                     /gene="cbrA"
                     /locus_tag="RHX88_001169"
     CDS             complement(17930..18994)
                     /gene="cbrA"
                     /locus_tag="RHX88_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDV2229892.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            19095..20309
                     /locus_tag="RHX88_001170"
     CDS             19095..20309
                     /locus_tag="RHX88_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDV2229893.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDNNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20311..20643)
                     /locus_tag="RHX88_001171"
     CDS             complement(20311..20643)
                     /locus_tag="RHX88_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV2229894.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYLGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20883..20955
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20949..21362
                     /gene="ibpA"
                     /locus_tag="RHX88_001172"
     CDS             20949..21362
                     /gene="ibpA"
                     /locus_tag="RHX88_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDV2229895.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21474..21902
                     /gene="ibpB"
                     /locus_tag="RHX88_001173"
     CDS             21474..21902
                     /gene="ibpB"
                     /locus_tag="RHX88_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDV2229896.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            22099..23760
                     /locus_tag="RHX88_001174"
     CDS             22099..23760
                     /locus_tag="RHX88_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDV2229897.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23757..24473)
                     /locus_tag="RHX88_001175"
     CDS             complement(23757..24473)
                     /locus_tag="RHX88_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2229898.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24769..26385
                     /locus_tag="RHX88_001176"
     CDS             24769..26385
                     /locus_tag="RHX88_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV2229899.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26385..27199
                     /locus_tag="RHX88_001177"
                     /pseudo
     CDS             26385..27199
                     /locus_tag="RHX88_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27196..28089)
                     /locus_tag="RHX88_001178"
     CDS             complement(27196..28089)
                     /locus_tag="RHX88_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV2229900.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28256..29971
                     /locus_tag="RHX88_001179"
     CDS             28256..29971
                     /locus_tag="RHX88_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDV2229901.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29968..31461
                     /locus_tag="RHX88_001180"
     CDS             29968..31461
                     /locus_tag="RHX88_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDV2229902.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPLEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31508..31957)
                     /locus_tag="RHX88_001181"
     CDS             complement(31508..31957)
                     /locus_tag="RHX88_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229903.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            32067..32414
                     /locus_tag="RHX88_001182"
     CDS             32067..32414
                     /locus_tag="RHX88_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDV2229904.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32404..32766
                     /locus_tag="RHX88_001183"
     CDS             32404..32766
                     /locus_tag="RHX88_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDV2229905.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32763..33260
                     /locus_tag="RHX88_001184"
     CDS             32763..33260
                     /locus_tag="RHX88_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDV2229906.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33268..34452)
                     /gene="emrD"
                     /locus_tag="RHX88_001185"
     CDS             complement(33268..34452)
                     /gene="emrD"
                     /locus_tag="RHX88_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDV2229907.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(34871..34960)
                     /gene="tisB"
                     /locus_tag="RHX88_001186"
     CDS             complement(34871..34960)
                     /gene="tisB"
                     /locus_tag="RHX88_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDV2229908.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35525..35623
                     /gene="ivbL"
                     /locus_tag="RHX88_001187"
     CDS             35525..35623
                     /gene="ivbL"
                     /locus_tag="RHX88_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDV2229909.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35729..37417
                     /gene="ilvB"
                     /locus_tag="RHX88_001188"
     CDS             35729..37417
                     /gene="ilvB"
                     /locus_tag="RHX88_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDV2229910.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37421..37711
                     /gene="ilvN"
                     /locus_tag="RHX88_001189"
     CDS             37421..37711
                     /gene="ilvN"
                     /locus_tag="RHX88_001189"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV2229911.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37786..38376
                     /gene="uhpA"
                     /locus_tag="RHX88_001190"
     CDS             37786..38376
                     /gene="uhpA"
                     /locus_tag="RHX88_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDV2229912.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38376..39878
                     /gene="uhpB"
                     /locus_tag="RHX88_001191"
     CDS             38376..39878
                     /gene="uhpB"
                     /locus_tag="RHX88_001191"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDV2229913.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLSLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39888..41207
                     /gene="uhpC"
                     /locus_tag="RHX88_001192"
     CDS             39888..41207
                     /gene="uhpC"
                     /locus_tag="RHX88_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDV2229914.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            41345..42736
                     /gene="uhpT"
                     /locus_tag="RHX88_001193"
     CDS             41345..42736
                     /gene="uhpT"
                     /locus_tag="RHX88_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDV2229915.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42781..>44526)
                     /gene="adeD"
                     /locus_tag="RHX88_001194"
     CDS             complement(42781..>44526)
                     /gene="adeD"
                     /locus_tag="RHX88_001194"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDV2229916.1"
                     /translation="KFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGP
                     IVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLP
                     RGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFT
                     LEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNA
                     YITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCT
                     DDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQA
                     DIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFA
                     LQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLL
                     GGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLP
                     LPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGL
                     FDGEKFAFTTLEVTE"
BASE COUNT        10241 a        11459 c        12100 g        10726 t
ORIGIN      
        1 aattgtttaa atgtgaatcg aatcacaatc gttcggggag caatattaaa tgcttttttt
       61 taagattata aattacgtaa gaattttaat gtttaaaaaa agggttgagg aataacagga
      121 gtgctaacaa tgaagcgaag ccgcatctga cagtcagaat acggcttcgt aagcgcggtt
      181 acttaccaat acagaagctg gagaaaatcc gccccagcag gtcgtctgaa gtaaattccc
      241 cggtgatttc gcttaagttc tgctgtgcca ggcgcaactc ttccgccagc agttcacccg
      301 cccaggcacc taacagttgc gctttacctt gctgcaaatg ctccgctgcc tgttccagcg
      361 cctgtaagtg gcgacggcgc gccaggaaac cgccttccat gttggtgtca aagcccatgc
      421 tctgtttgag atggttacgc agcacgtcca cgccttcacc agtccttgcc gagagacgaa
      481 ttaacgcgtg accgttcact tcactcattc ccagcgtttc gccggtgata tcggctttat
      541 tgcgcaccac ggtgatcggc agtttcgctg gcagacgggc gataaattcc ggccagatct
      601 ctgccggatc cacggcgtct gttgtggtgc catcgaccat aaacagcacc cggtcggcct
      661 gttcaatttc ctgccacgcg cgctcgatcc caatacgttc cacttcgtca ctggcttcac
      721 gtagcccggc ggtgtcgatg atatgcagcg gcattccgtc aatgtggata tgctcacgca
      781 acacgtcacg cgtagttccg gcgatatcgg ttacgattgc tgcttcacgg ccagccagcg
      841 cgtttaacag gctcgattta ccggcgttag gacgtccggc aatcaccact ttcatccctt
      901 cgcgcaacaa actaccctga cgtgcttcag cacgcactgc atcaagatcg gcaataacgt
      961 cattgagctg ggcttcaatt ttgccgtcgg agaggaaatc gatctcttca tcagggaaat
     1021 caattgccgc ttcgacgtaa atgcgcaagt gggtgagggc ttccaccaga tggttgaccc
     1081 gtgcggagaa tgcgccttgc agcgagttaa gtgccgaacg ggccgcctgt tccgaactgg
     1141 cgtcgataag atcggcaatc gcctcggcct gggctaagtc gagtttatcg ttaagaaacg
     1201 cgcgttcgga aaactcacca gggcgagcaa tccgcaggcc gggaatggtc agaatgcgtt
     1261 ttaacagcag gtcgaggatc accggaccgc catgaccttg cagttccagc acatcttcgc
     1321 cggtgaacga gttcgggcca gggaaccata gcgcaatccc ctgatcgagc acgctgccgt
     1381 cggcgtcttt aaacggaaga taatcggcgt agcgcggctt aggtagttta cccagcacgg
     1441 tttcggcaac ttcacgggct ttgaagccgg agatgcgcag gatgccaacg ccgccacgtc
     1501 ccggaggcgt ggcctgggct acgatagtgt cattatcgct catgatgttc ctgttgcttt
     1561 gtgtggcgga tgcgcggtgc ttatccgccc tacgaaaaga aaaaaggcgg tcaactgacc
     1621 gcccttattt tagcgaaaac tcaccgaatc aggatttttt cttctcgcgg ctatgcaggc
     1681 cacgtttttc cagaccacgg taaatcagct gctgctgaat aatggttacc aggttgctga
     1741 cgatatagta cagcaccaga cctgacggga accacaggaa gaacacggtg aagatgaccg
     1801 gcataaaggt catgatcttc tgctgcatcg ggtcggtcac tgtggtcggc gacatcttct
     1861 gaatgaagaa catcgttacg cccatcagga tcggcaagat gtagtacggg tcctgtgccg
     1921 acaggtcgtg gatccacagt gcaaacggtg cctgacgcag ttcaacggaa cccatcagca
     1981 tgtagtacaa cgccaggaag attggcatct ggatcagcag cgggaagcag ccgcccagcg
     2041 ggttaacctt ctcagctttg tacagcgcca tcatttcctg gctgatacgc tgtttgtcat
     2101 cgcccagacg ctcacgcatt gcctgaatct tcggctgcag catacgcatc ttcgccatgg
     2161 aggtgtactg cgctttggtc agcgggtaca tgatgccacg aacgataaag gtgatgatga
     2221 taatggagaa gccccagtta cccacaaagc tatggatcca tttcagcagt ttgaacagcg
     2281 gctgagagat gaaccacaac caaccgtaat caacggtcag atccaggtgc ggagcaacag
     2341 ctgccatttt gtcctggatt tccgggccaa cccacagggt gctgttcatc gcgccagtct
     2401 gaccaggctg aaccagtacc ggctgagatt tatagccgat agcggcgatg ccgttaccca
     2461 gattagcggt atagaagttg ttggtaccgt cgttatgcgg gatccacgcc gtcgcgaaat
     2521 actgttgcag catcgccacc caaccacctt tcgaagagat gttcaggttt tcgttatcgg
     2581 caatggtatc gaacttgtat ttctcgtact tctcgtcagg cgtggagtac gccgcgccgc
     2641 ggaaggtgtg cagtgcgaag ttgctgcttc cggtatcgag atgcggtggc agagtgatgg
     2701 attgcttcaa ctgaccaaag gtggagattt ccagcggttt ctcgccagcg ttctgcacgt
     2761 tgtagttgac gttgacagcg taatcaccac gtttcaggac aaacgttttg gtaaacgtgt
     2821 tgcctgccgc gtcggtatac gtcatcggca cctgcagttc gttttgacct tcagccagca
     2881 cataagcgtc tttttcaacg ttatacagcg gacgcgggcc gttagccggg ttatccgggc
     2941 catcacgacc ggtcagaccg ctctgtgcct gataaataaa ctgcggtgaa gtttccagca
     3001 gctggaacgg ctgggtagag ttcagctctt tcgggtaagc aggcagcaga gcttgctcaa
     3061 catcaccacc acgggtgttg atggtcagat caagcacgtc ggtcttaacc gagatcagtt
     3121 tcccctggcc actggccggt acgccctggt cggcggcgct acccgctgcg gtggtcgttg
     3181 tctgcgtggt ctgttgggcc tgaggttgcg ggtttttatc ctgctcccag gcttgccaga
     3241 tcatgaaaga cacgaacagc aaagcgatga ctaaaagatt gcgttgcgaa tccatcgtta
     3301 gtgttctctg gtatcaaatg gtccgggcgg gacgggatcg tcaccaccag ggtgtaaagg
     3361 gtggcatttt aatacgcgtt tcaccgtcaa ccaactgcct tttatcactc caaacctgcg
     3421 caatgcctca attccgtagc ttgaacaggt tggagtgaaa cgacaatgcg gcccgagtag
     3481 cggactaatc aggcgttgat agacccgaat gagggctatc aggacccgcg agccaggcga
     3541 cagtggcggc gccataattt ttccaacgct tccgagagag cacggttatc gaggtcggca
     3601 accccttttt tcgccaccac cacgaaatcc atagccggga gttcatgttg gcgcagacgg
     3661 aagctttcac gcgtcagacg tttaatccga ttgcgttcat gggcgcgtcg aacgtttttc
     3721 ttggcgactg taagaccgat acggggatgc cccagcgaat tcaggcggcc gagaatggta
     3781 atttgcggcg tgccagcccg ttgtggctgc tggaagacga atgtgaattg actgggagtt
     3841 aacaagcgta actccctggg aaatgcgagc ttaaccactc aggggttagc tttattactt
     3901 agaaacggtc agacgagcgc ggcctttagc acgacgacgt gccagaacct gacgaccatt
     3961 tttagtagcc atacgagcac ggaagccgtg agaacggttg cgcttcagta cagacggttg
     4021 aaaagtgcgt ttcatggcga tttctaccta aacttgaata aattcaatgg ctttattgga
     4081 tatccgccga aaaatgaaac gatggacacc gaagccatgg gtgattaaag aggccggatt
     4141 gtaataattg tacactccgg agtcaattct ctttccttat ttaccgcgct tttccgcacc
     4201 ttttcgcagg gaaaatgtac gacctcacac cagtgaaaac cagcatggcg cgccgggtgg
     4261 aggattatac gggctgatgg gtaaagcgca aggatcgtcc tggatcttta ttagatcgat
     4321 taagccaatt tttgtctatg gtcattaaat tttccaatat gcggcgtaaa tcgtgcccgc
     4381 ctcgcggcag gatcgtttac acttagcgag gtctggaaag tcctgtggat aaatcgggaa
     4441 aatctgtgag aaacagaaga tctcttgcgc agtttaggct atgatccgcg gtcccgatcg
     4501 ttttgcagga tcttgatcgg gcatataacc gcagacagcg gttcgtgcgt caccctcaag
     4561 cagggtcttt tcgacgtacg tcaacaatca tgaatgtttc agccttagtc attatcgact
     4621 tttgttcgag tggagtccgc cgtgtcactt tcgctttggc agcagtgtct tgcccgattg
     4681 caggatgagt taccagccac agaattcagt atgtggatac gcccattgca ggcggaactg
     4741 agcgataaca cgctggccct gtacgcgcca aaccgttttg tcctcgattg ggtacgggac
     4801 aagtacctta ataatatcaa tggactgcta accagtttct gcggagcgga tgccccacag
     4861 ctgcgttttg aagtcggcac caaaccggtg acgcaaacgc cacaagcggc agtgacgagc
     4921 aacgtcgcgg cccctgcaca ggtggcgcaa acgcagccgc aacgtgctgc gccttctacg
     4981 cgctcaggtt gggataacgt cccggccccg gcagaaccaa cctatcgttc taacgtaaac
     5041 gtcaaacaca cgtttgataa cttcgttgaa ggtaaatcta accaactggc gcgcgcggcg
     5101 gctcgccagg tggcggataa ccctggcggt gcctataacc cgttgttcct ttatggcggc
     5161 acgggtctgg gtaaaactca cctgctgcat gcggtgggta acggcattat ggcgcgcaag
     5221 ccgaatgcca aagtggttta tatgcactcc gagcgctttg ttcaggacat ggttaaagcc
     5281 ctgcaaaaca acgcgatcga agagtttaaa cgctactacc gttccgtaga tgcactgctg
     5341 atcgacgata ttcagttttt tgctaataaa gaacgatctc aggaagagtt tttccatacc
     5401 ttcaacgccc tgctggaagg taatcaacag atcattctca cctcggatcg ctatccgaaa
     5461 gagatcaacg gcgttgagga tcgtttgaaa tcccgcttcg gttggggact gactgtggca
     5521 atcgaaccgc cagagctgga aacccgtgtg gcgatcctga tgaaaaaggc cgacgaaaac
     5581 gacattcgtt tgccgggcga agtggcgttc tttatcgcca agcgtctacg atctaacgta
     5641 cgtgagctgg aaggggcgct gaaccgcgtc attgccaatg ccaactttac cggacgggcg
     5701 atcaccatcg acttcgtgcg tgaggcgctg cgcgacttgc tggcattgca ggaaaaactg
     5761 gtcactatcg acaatattca gaagacggtg gcggagtact acaagatcaa agtcgcggat
     5821 ctcctttcca agcgtcgatc ccgctcggtg gcgcgtccgc gccagatggc gatggcgctg
     5881 gcgaaagagc tgactaacca cagtctgccg gagattggcg atgcgtttgg tggtcgtgac
     5941 cacacgacgg tgcttcatgc ctgccggaag atcgagcagt tgcgtgaaga gagccacgat
     6001 atcaaagaag atttttcaaa tttaatcaga acattgtcat cgtaaaccta tgaaatttac
     6061 cgtagaacgt gagcatttat taaaaccgct acaacaggtg agcggtccgt taggtggtcg
     6121 tcctacgcta ccgattctcg gtaatctgct gttacaggtt gctgacggta cgttgtcgct
     6181 gaccggtact gatctcgaga tggaaatggt ggcacgcgtt gcgctggttc agccgcacga
     6241 acctggtgcg acgaccgttc cggcgcgcaa attctttgat atctgccgtg gtctgcctga
     6301 aggcgcggaa attgccgtgc agctggaagg tgaacggatg ctggtgcgct ccgggcgtag
     6361 ccgtttttcg ctgtctaccc tgccagcggc ggatttcccg aacctcgatg actggcagag
     6421 cgaagtcgaa tttaccctgc cgcaggcgac gatgaagcgt ctgattgaag cgacccagtt
     6481 ttctatggcg catcaggacg ttcgctatta cttaaacggt atgctgtttg aaaccgaagg
     6541 tgaagaactg cgcaccgtgg cgaccgatgg ccaccgtctg gcggtctgtt caatgccaat
     6601 tggtcaatct ttgccaagcc attcggtgat cgtgccgcgt aaaggcgtga ttgaactgat
     6661 gcgtatgctc gacggcggcg acaatccgct gcgcgtgcag attggcagca acaacattcg
     6721 cgcccacgtt ggtgacttta tcttcacctc caaactggtg gatggtcgct tcccggatta
     6781 tcgccgcgtt ctgccgaaga acccggacaa acatctggaa gctggctgcg atctgctcaa
     6841 gcaggcgttt gcccgtgcgg caattctctc taacgagaaa ttccgcggcg tgcgcctgta
     6901 tgtcagcgaa aaccagctga aaatcaccgc caacaacccg gaacaggaag aagcggaaga
     6961 gatcctcgac gttacctata gcggtgcgga gatggaaatc ggcttcaacg tcagctatgt
     7021 gctggatgtt ctgaacgcgc tgaaatgcga aaacgtccgc atgatgctga ccgattcggt
     7081 ttccagcgtg cagattgaag atgccgcatc acagtcggct gcctatgttg tcatgccaat
     7141 gagactgtaa tgtccctcac ccgcttgttg atccgcgatt tccgcaacat tgaaaccgcg
     7201 gatctcgctt tatctcccgg ctttaacttt ctggtaggtg ccaacggcag tggcaaaacc
     7261 agcgtgctgg aagccatcta tacgctcggc catggtcggg cgtttcgcag tttgcagatt
     7321 ggtcgcgtca ttcgccatga gcaggaggca tttgttctcc atgggcgatt acagggcgaa
     7381 gagcgcgaga cggcgattgg cttaaccaag gacaaacagg gcgacagcaa agtccgcatc
     7441 gacggtactg acgggcataa agtcgcggaa ctggcgcacc tgatgccaat gcagctgata
     7501 acgccagaag ggtttacttt actcaacggc ggccccaaat acagaagagc attcctcgac
     7561 tggggatgct ttcacaacga acccggattt ttcaccgcct ggagcaatct caagcgattg
     7621 ctcaagcagc gcaatgcggc gctgcgccag gtgacacgtt acgaacagct acgcccgtgg
     7681 gataaagagc tgatcccgct ggcggagcaa atcagcacct ggcgcgcgga gtatagcgcc
     7741 ggtatcgcgg ctgatatggc cgatacctgt aagcaatttc tccctgagtt ttctctgact
     7801 ttctctttcc agcgcggctg ggagaaagag acagaatatg ctgaggtgct ggaacgtaat
     7861 tttgaacgcg atcgccagct aacctacacc gcgcacggcc cgcataaagc ggacttacgc
     7921 attcgcgccg acggtgctcc ggtggaagat accttatcgc gtgggcagct taagctgttg
     7981 atgtgcgcct tacgtctggc gcaaggagag ttcctcaccc gtgaaagcgg gcggcggtgt
     8041 ctctacctga tagatgattt tgcctctgag cttgatgatg agcgtcgcgg gttgcttgcc
     8101 agccgcttaa aagcgacgca atcacaggtc tttgtcagcg cgatcagtgc tgaacacgtt
     8161 atagacatgt cggacgaaaa ttcgaagatg tttaccgtgg aaaagggtaa aataacggat
     8221 taacccaagt ataaatgagc gagaaacgtt gatgtcgaat tcttatgact cctccagtat
     8281 caaagtcctg aaagggctgg atgcggtgcg taagcgcccg ggtatgtata tcggcgacac
     8341 ggatgacggc accggtctgc accacatggt attcgaggtg gtagataacg ctatcgacga
     8401 agcgctcgcg ggtcactgta aagaaattat cgtcaccatt cacgccgata actctgtctc
     8461 tgtacaggat gacgggcgcg gcattccgac cggtattcac ccggaagagg gcgtatcggc
     8521 ggcggaagtg atcatgaccg ttctgcacgc aggcggtaaa tttgacgata actcctataa
     8581 agtgtccggc ggtctgcacg gcgttggtgt ttcggtagta aacgccctgt cgcaaaaact
     8641 ggagctggtt atccagcgcg agggtaaaat tcaccgtcag atctacgaac acggtgtacc
     8701 gcaggccccg ctggcggtta ccggcgagac tgaaaaaacc ggcaccatgg tgcgtttctg
     8761 gcctagcctc gaaaccttca ccaatgtgac cgagttcgaa tatgaaattc tggcgaaacg
     8821 tctgcgtgag ttgtcgttcc tcaactccgg cgtttccatt cgtctgcgcg acaagcgcga
     8881 cggcaaagaa gaccacttcc actatgaagg cggcatcaag gcgttcgttg aatatctgaa
     8941 caagaacaaa acgccgatcc acccgaatat cttctacttc tccaccgaaa aagacggtat
     9001 tggcgtcgaa gtggcgttgc agtggaacga tggcttccag gaaaacatct actgctttac
     9061 caacaacatt ccgcagcgtg acggcggtac tcacctggca ggcttccgtg cggcgatgac
     9121 ccgtaccctg aatgcctaca tggacaaaga aggctacagc aaaaaagcca aagtcagcgc
     9181 caccggtgac gatgcgcgtg aaggcctgat tgcggtcgtt tccgtgaaag tgccggaccc
     9241 gaaattctcc tcccagacca aagacaaact ggtttcttct gaggtgaaat cggcggttga
     9301 acagcagatg aacgaactgc tggcagaata cctgctggaa aacccaaccg acgcgaaaat
     9361 cgtggttggc aaaattatcg atgctgcccg tgcccgtgaa gcggcgcgtc gcgcgcgtga
     9421 aatgacccgc cgtaaaggtg cgctcgactt agcgggcctg ccgggcaaac tggcagactg
     9481 ccaggaacgc gatccggcgc tttccgaact gtacctggtg gaaggggact ccgcgggcgg
     9541 ctctgcgaag caggggcgta accgcaagaa ccaggcgatt ctgccgctga agggtaaaat
     9601 cctcaacgtc gagaaagcgc gcttcgataa gatgctctct tctcaggaag tggcgacgct
     9661 tatcaccgcg cttggttgtg gtatcggtcg tgacgagtac aacccggaca aactgcgtta
     9721 tcacagcatc atcatcatga ccgatgcgga cgtcgacggc tcgcacattc gtacgctgct
     9781 gttgaccttc ttctatcgtc agatgccgga aatcgttgaa cgcggtcacg tctacatcgc
     9841 tcagccgccg ctgtacaaag tgaagaaagg caagcaggaa cagtacatta aagacgacga
     9901 agcgatggat cagtaccaga tctctatcgc gctggacggc gcaacgctgc acaccaacgc
     9961 cagtgcaccg gcattggctg gcgaagcgtt agagaaactg gtatctgagt acaacgcgac
    10021 gcagaaaatg atcaatcgta tggagcgtcg ttatccgaaa gcaatgctga aagagcttat
    10081 ctatcaaccg acgttgacgg aagctgacct ctctgatgag cagaccgtta cccgctgggt
    10141 gaacgcgctg gtcagcgaac tgaacgacaa agaacagcac ggtagccagt ggaagtttga
    10201 tgtccacacc aatgccgagc aaaacctgtt cgagccgatt gttcgcgtgc gtacccacgg
    10261 tgtggatact gactatccgc tggatcacga gtttatcacc ggtggcgaat atcgtcgtat
    10321 ctgcacgctg ggtgagaaac tgcgtggctt gctggaagaa gatgcgttta tcgaacgtgg
    10381 cgagcgtcgt cagccggtag ccagcttcga gcaggcgctg gactggctgg tgaaagagtc
    10441 ccgtcgcggc ctctccatcc agcgttataa aggtctgggc gagatgaacc cggaacagct
    10501 gtgggaaacc accatggacc cggaaagtcg tcgtatgctg cgcgttaccg ttaaggatgc
    10561 gattgctgct gaccagctgt tcaccacgct gatgggcgac gccgttgaac cgcgccgtgc
    10621 gtttattgaa gagaacgccc tgaaagcggc gaatatcgat atttaatggc gttaaccatg
    10681 cgagcgtgcc tgatgcgcta cgcttatcag gcctacgaga atcttgcaat gtattgaatt
    10741 ttcatgattt tgtaggccgg gtaaggcgtt agcgccgcat ctggcaagaa gctataagaa
    10801 aagggcggag atcatctccg ccctttttat ttctgcaatc cgcaattatc atcccgcaaa
    10861 tctatacttt ttgggtcaac acgtaacgct ttacaggtga ttatcatggg gctttttgat
    10921 gaagttgtcg gtgcctttct gaaaggcgat gcggggaaat atcaggctat tttaagttgg
    10981 gttgaggagc agggcggcat tcaggtgctg ctggaaaaac tgcaaagtgg cggcttaggg
    11041 gccattctct caacctggct gagtaatcaa cagggcaatc aatcggttag tggcgagcaa
    11101 ctggaatcgg cgctcggcac aaatgcggtg tccgatctcg ggcaaaaact tggcgtggat
    11161 accagtacag cttccagttt actggcagag caattgccga agattattga tgcgctctca
    11221 ccgcaaggtg aagtgtcacc acaagccaat aacgatctgc tttccgcagg tatggaactg
    11281 ctgaaaggga aactcttccg ctaagcaaat ggggagcatg caacccgcgt tgcatccccc
    11341 ttttccctcg ggtgttgtac tgatttttga gcggaatcgc gttagcatgg gtcaggaacc
    11401 aatctacctg gggaactcat ggctatcaaa ctcattgcta tcgatatgga tggcaccctt
    11461 ctgctgcccg atcacaccat ttcacccgcc gttaaaaatg cgattgccgc agctcgcgcc
    11521 cgtggcgtga atgtcgtgct aacgacgggt cgcccttatg caggcgttca caactacctg
    11581 aaagagctgc atatggaaca gccgggcgac tactgcatta cttataacgg cgcgttggta
    11641 cagaaggccg ccgatggtag caccgtggcg caaaccgctc tcagctatga cgactaccgt
    11701 ttcctggaaa aactctctcg cgaagtcggt tctcatttcc acgccctgga ccgcaccacg
    11761 ctttatacag caaatcgcga tatcagctac tacacggtgc atgaatcctt cgttgccacc
    11821 attccgctgg tgttctgcga agcggagaaa atggacccca atacccagtt cctgaaagtg
    11881 atgatgattg atgaacccgc catcctcgac caggctattg cgcgtattcc gcaggaagtg
    11941 aaagagaaat ataccgtgct gaaaagtgcg ccgtacttcc tcgaaatcct cgataaacgc
    12001 gttaacaagg gtacgggtgt gaaatcactg gccgacgtgt taggtattaa accggaagaa
    12061 atcatggcga ttggcgatca ggaaaacgac atcgcaatga ttgaatatgc aggcgtcggt
    12121 gtggcgatgg ataacgctat tccttcggtg aaagaagtgg cgaactttgt caccaaatcc
    12181 aaccttgaag atggcgtggc gtttgctatt gagaagtatg tgctgaatta atcagtggac
    12241 gggcaaacag cataaacgcg tttgcccgct tactgattat tggtttactg catatacaac
    12301 cagaaattgc agcggtgcag taatgatgtc cagcgtaaca gtcactggca gtaatgctgc
    12361 ggaaacacca tccggaatgc ctctgggtga atagtattca taaaatcgca ctttaaacgg
    12421 atgatagaac gccatcacct ttgagtaatc catgttttta tttttctcat ggattgtgcc
    12481 ttttaggttt tccagtgacc tggtacagtg ttgaacacca caggcaaaac cataatgcgt
    12541 tgccagtgcc ttttcttctt cgttattcca gtagtagtaa agcgatattg tgccggtaaa
    12601 tttctttttc cccgcattta aaacaaatct tgcgtcatcg gcaacctgaa tattgtgccg
    12661 gttaagtgct ggatcattga gcatcttaac gatgtcattg gcaccatcag tgataaggta
    12721 atccagcgat tcccctacaa aaacatagcc ttttgttcca ttactatctt ttgcctgcga
    12781 tagcccagtg atggtgtctg actgcgattg atactttcct gaactaccct ttgcggtatg
    12841 ggaaaccaga gcatacgaga tacagcctga aagcatcagc gccagtggga ataaaccggc
    12901 agccttaaaa aatcccttaa aattcaactt catgattaat cctttaaacg agtagctaag
    12961 ccgttgaaag catactcttc tttccctcct gtttagcgcc tgatatccct tttcagattt
    13021 ctgcccgacg catgtcattt ttttatgcat tgttcttttt gtgatctaaa ttgtagtaca
    13081 acaatataag tttgtactac attacgcaca cggcaaacgc gaacgtcatc acgctggtac
    13141 tacaaagttg ccgcgttatg catcgatcgg ggtaaagtag agaagaacat acagagcaca
    13201 aggactctcc atgactctca ataaaaccga tcgcattgtc attacgctgg gtaaacagat
    13261 cgttcacggc aaatacgtac ctggctcgcc acttccggct gaggcggagc tctgtgaaga
    13321 gtttgcaacc tcgcgcaaca tcatccgtga ggtgttccgt tcgttgatgg cgaagcggct
    13381 gattgaaatg aaacgttatc gcggcgcgtt tgtggcaccg cgtaaccagt ggaattacct
    13441 cgacactgac gtactgcaat gggtgctgga aaacgactac gacccacggc ttatcagtgc
    13501 catgagcgaa gtgcgaaatc tggtggaacc ggcgattgcc cgttgggcag cagagcgcgc
    13561 gacctccagc gatctggcgc agattgaatc ggcgctgaac gagatgattg ccaacaatca
    13621 ggaccgcgaa gcgtttaacg aagcggatat tcgctaccac gaggcggtgc tgcagtcggt
    13681 gcataacccg gtgttacagc aacttagcat tgcgatcagt tcgctgcagc gggcggtttt
    13741 tgaacgaacc tggatgggcg atgaggccaa catgccgcaa acgctccagg aacataaggc
    13801 gctgttcgat gcgatacgac atcaggacgg cgatgcggca gagcaggcgg cgcttaccat
    13861 gatcgccagc tcgacacgaa ggttaaagga aatcacatga cagctcgcta catcgcaatt
    13921 gactggggat cgaccaatct gcgtgcctgg ctttatcagg gcgaccactg cctggacagc
    13981 aggcaatcag aagcaggcgt cacgcgcctg aacggaaaat ctccggctgc ggtgttagca
    14041 gaagtcacga ccgactggcg tgaagagaat acgccagtgg tgatggcagg aatggtcggc
    14101 agcaatgtcg gctggaaagt tgcaccgtat ttatctgttc ctgcccgttt ttcgtctatt
    14161 ggcgatcaat taacgtcggt tggcgacaat atctggatta ttcccggatt atgcgtctct
    14221 catgacgata accacaatgt gatgcgcggc gaagaaacac aattgatcgg cgcgcgcact
    14281 ctggctcctt cctctcttta tgtcatgccc ggaacccatt gcaaatgggt gcaggccgat
    14341 agccagcaaa tcaacgattt tcgcaccgtg atgaccggtg aattacatca tttactgtta
    14401 aatcactcat tgattggcgc aggtttgccg ccgcaggaaa actctgccga tgccttcgcg
    14461 gctggccttg agcgtggcct taatgcgccc gccatattgc cgcagctttt tgaagttcgc
    14521 gcctcgcatg tgctgggaac acttccccgc gaacaggtca gcgaatttct ctctggtttg
    14581 ttgattggcg cagaggtcgc cagtatgcgc gactatgtgg cccatcaaca cgccatcacc
    14641 cttgtcgccg gaacatcgct gaccgcgcgc taccagcaag cctttcaggc gatgggttgc
    14701 gacgtgacag cggtagcagg cgacacggca tttcaggctg gtataaggag catcgctcat
    14761 gcagtggcaa actaaactcc cgctgatcgc cattttgcgc ggcattaagc ccgacgaggc
    14821 gctggcgcat gttggcgcgg tgattgacgc cgggttcgac gcggttgaaa tcccgctgaa
    14881 ttccccacaa tgggagcaaa gcattcccgc catcgttgat gcgtatggcg acaaggcgtt
    14941 gattggcgca ggtacggtac tgaaacctga acaggtcgat gcgctcgcca ggatgggctg
    15001 tcagctcatc gttacgccca atatccatag tgaagtgatc cgccgtgcgg tgggctacgg
    15061 catgaccgtc tgccccggct gcgcgacggc gaccgaagcc tttaccgcgc tcgaagcggg
    15121 cgcgcaggcg ctgaaaatat ttccgtcatc ggcttttggt ccgcaataca tcaaagcgtt
    15181 aaaagcggta ttgccatcgg acatcgcagt ctttgccgtt ggcggcgtga cgccagaaaa
    15241 cctggcgcag tggatagacg caggttgtgc tggggcgggc ttaggcagcg atctctatcg
    15301 cgccgggcag tccgtagaac gcaccgcgca gcaggcagca gcatttgtta aggcgtatcg
    15361 agaggcagtg caatgaaaat caccaaaatt accacgtatc gtttacctcc ccgctggatg
    15421 ttcctgaaaa ttgaaaccga tgaaggcgtg gtcggttggg gcgagcccgt gattgaaggc
    15481 cgcgcccgta cggtggaagc ggcagttcac gagctgggtg actatttgat tggtcaggat
    15541 ccttcgcgca tcaacgactt atggcaagtg atgtatcgcg ccggatttta tcgtggcggt
    15601 ccaatcctga tgagcgccat cgccgggatc gaccaggcgt tatgggatat caaaggcaaa
    15661 gtgctgaatg cgccagtctg gcaactgatg ggcggcctgg ttcgcgacaa aattaaagcc
    15721 tacagttggg tcggcggcga tcgtccggcg gatgttatcg acggcattaa aaccctgcgc
    15781 gaaatcggct tcgatacctt caaactgaac ggttgtgaag aactggggct aattgataac
    15841 tcccgcgcgg tagatgcggc agtcaacacc gtggcacaaa ttcgtgaagc ttttggcaat
    15901 cagattgagt ttggtcttga tttccacggt cgcgtcagcg cgccgatggc gaaagtgctg
    15961 attaaagaac tggagccata tcgcccgctg tttattgaag agccggtgct ggcggaacaa
    16021 gccgaatact acccgaaact ggcggcacaa acgcatattc cgctggcggc gggtgagcgc
    16081 atgttctcac gtttcgattt taaacgcgtg ctggaggcag gcggtatttc gattctacaa
    16141 ccggatctct cccatgcagg cggtattacc gaatgctaca aaattgccgg aatggcagaa
    16201 gcctatgacg tgacccttgc gccgcactgt ccgctcggac cgattgcact ggcggcttgt
    16261 ctgcatatcg actttgtttc ctataacgcg gtacttcagg aacaaagtat gggaattcat
    16321 tacaacaaag gcgcggagtt actcgacttt gtgaaaaaca aagaagactt cagcatggtt
    16381 ggcggcttct ttaaaccgtt aacgaaaccg ggcttaggcg tggaaatcga cgaagctaaa
    16441 gttattgagt tcagtaaaaa tgccccggac tggcgtaatc cgctctggcg tcatgaagat
    16501 aacagcgtag cagagtggta attcctgcca cgtaagcccc tcatcgggca ctaaaacagc
    16561 aatacaaaaa tataaccctc tgtaaattac agggcatggt gagcggcttc gctatgccca
    16621 aaatctggag aaagctcgct atggatattc ccgttaatgc agcaaagccg gggcgtcggc
    16681 gttatctgac gctggtgatg atctttatta cggtggtcat ttgttatgtt gaccgcgcta
    16741 acctggccgt ggcttccgcc catattcagg aagagttcgg cattacgaaa gcggaaatgg
    16801 gctatgtatt ttcggccttc gcctggcttt atacgctatg ccagatcccc ggcggttggt
    16861 ttttagatcg cgtaggttct cgcgtgactt attttattgc gatatttggc tggtcagtgg
    16921 cgactttatt ccagggcttt gccacgggat taatgtcatt aattggtctg cgcgcgataa
    16981 ccggtatttt cgaagcgccc gctttcccga ccaataaccg gatggtgacc agctggttcc
    17041 cggaacatga acgcgcttcc gccgttggtt tttatacgtc tggtcagttt gtcggtctgg
    17101 cgtttttgac tccgctgctg atctggattc aggagatgtt gagctggcac tgggtgttca
    17161 ttgtcactgg tggtatcggc attatctggt cgctgatttg gtttaaggtt tatcagccgc
    17221 cgcgcctgac caaaggtatc agcaaagctg aactggatta cattcgtgat ggcggcggtc
    17281 tggtggatgg tgatgcgccg gtgaaaaaag aggcacgtca gcccttaaca gccaaagact
    17341 ggaagctggt gttccatcgt aaactgatcg gcgtttatct tgggcaattt gcggtggctt
    17401 ctacactgtg gtttttctta acctggttcc cgaactattt aacccaggaa aaaggaatca
    17461 cggcgctgaa agcaggcttt atgaccacgg taccgttcct cgcggcgttt gtcggcgtcc
    17521 tgctctctgg ctgggtcgcg gatctgctgg tacgtaaggg cttttcactg ggctttgcgc
    17581 gtaaaacgcc gattatctgc ggcttgctga tctccacctg cattatgggc gctaactaca
    17641 ctaacgatcc gatgatgatt atgtgcctga tggcgctggc attcttcggc aacggttttg
    17701 cttcgattac ctggtcgctg gtctcttctc tggcaccgat gcgcctgatt ggtttaaccg
    17761 gcggcgtgtt taacttcgcc ggtggtctgg gcggcatcac cgttccgctg gtggtggggt
    17821 acctggcaca gggttacggt ttcgcacctg cactggttta tatctccgcc gtcgcgttga
    17881 ttggcgcgct ctcttacatc ctgctggtgg gcgatgtgaa gcgcgttggc taatctttta
    17941 actgtggaat atgcgccaca ccactgcgca taatccactt tcttaaagcc ggtgcggtca
    18001 ggcatcggct ttttactatc ttgccgaaga gttttaaacg cagtttgcgt gtggcgcgcc
    18061 agtaagcggc gttgctcttc tccggctgct tcagtaacac cgaacgcaga atctctgcgc
    18121 tatccagcgc atagctaatc ccctccagcg agctggcgct gataaatccc gccgcttcgc
    18181 caatcagaaa ggcgttgtcc ttaccgcaga caaaatcctg ccagcgtgag ggaaacagca
    18241 ccgtgcattt ttcgcttttc accgccttac caaactggaa ctgaaaggcg ctcattttct
    18301 ctttcagcgt cgtgaaacgc gtctgaccgt ctttcattgg ataagcaccg ccaaagataa
    18361 aataaccgtc tttgctgata ctccatgaat aacagttagt tatcgaatta tcaaagatgc
    18421 aggagtagaa cggcaccgga tgtttctccg cgaaccactg ctggatagcg acatatttac
    18481 ggatttgatg atccgggtag agatgtcgcc gcaccatcga gtttgcgcca tcggcaccga
    18541 ccagatagcg ggcggtaata tgctgctccc agccgtctgc acgaaaaatg acatgccatt
    18601 tatcatcctc acgccagatt tttcggcaca ggctatcgtg ataaacctca acgctggtgg
    18661 ggatcagcga tttcatccac aagtcgaaag catggcgatt aatattgata tagcttcgct
    18721 ggtagttacg cgtgagcgat gcggcgacgt cgacagtttt gacgctgaaa atctgtggat
    18781 tggcgatcac atcgacagga agcgtcagtc catcgcgaat aaaagatcgc tgcgcgtccg
    18841 gtgccagcag accgccgcaa ggtttgctga aaccttcagt accacactgg tgctttttat
    18901 ccagcgcgat cacctgcatt ttgcctgcta attttcgcgc caacgccgat cctgccgggc
    18961 cgaggccaat aatcgccacg tcgaaatgtt ccatcttttc actcacattc atcacgatgg
    19021 caaaagacta ttgtctgaaa gtgaagtcct tgtgaagtat gatggcggga cccgttttgt
    19081 atcaggaccg cgccatgaaa cagataacct ttgctccccg taatcacctg ctcaccaata
    19141 ccaatacctg gacgcccgac agccagtggc tggtatttga cgtgcgtcct tctggggcgt
    19201 cgtttaccgg cgagaccatt gagcgtgtga atatccatac cggcgaggtc gaggttatct
    19261 atcgcgcgtc acagggcgca cacgtcggcg tggtgaccgt tcatccgaag tcagagaaat
    19321 atgttttcat ccatggcccg gaaaatcctg atgaaacatg gcattacgat ttccatcatc
    19381 gacgcggagt cattgctgaa ggcggcaagg tgagcaatct cgatgcaatg gatattaccg
    19441 cgccgtatac gccaggagcg ctgcgcggcg gcagccatgt gcatgtcttt agcccgaacg
    19501 gtgaaagggt gagctttacc tataacgacc atgtaatgca tgaactcgat ccggcgctgg
    19561 atttgcgaaa cgtcggtgtt gctgcgccgt ttggcccggt caatgtacaa aagcagcatc
    19621 cgcgtgaata cagcggtagc cactggtgcg tgctggtgag taaaaccacg cccacgccgc
    19681 agcctggcag cgatgaaatc aatcgtgctt atgaagaagg atgggtagga aatcacgcgc
    19741 tggcgtttat tggcgacaca ctttcgccaa agggcgagaa agtgccggag ctgtttatcg
    19801 ttgagttacc gcaagatgaa gctggctgga aagcggcagg tgatgcgccg ttaagtggaa
    19861 cggaaaccac cctgcccgcg ccaccgcgcg gcgtcgtgca gcgacgttta acctttaccc
    19921 accatcgggc ttatccgggg ttagtcaacg tcccgcgcca ctgggtgcgc tgtaatccgc
    19981 agggtacgca aatcgcgttt ttaatgcgtg ataataacgg cattgtgcaa ctgtggctta
    20041 tctcgccaca gggcggcgag ccgcgccagt taacccataa caaaacggat attcagtctg
    20101 catttaactg gcatccgtca ggagaatggt tgggctttgt gctggataat cgaattgctt
    20161 gtgcccatgc gcaaagcggc gaggttgagt atttaaccga aaaccacgcc aatccacctt
    20221 ctgcggacgc cgtggtcttc tcgccggatg gtcaatggct ggcgtggatg gaaggtggcc
    20281 agctgtggat caccgaaact gatcgctaaa tcagttatca ggcattctgg ccggtggaat
    20341 aaccgcattg gtggcttgcg cgttggcttc gcttttttca acgcgtgagc gcaccgaact
    20401 gtctttgcgg aacacatccc acggcagtaa aagcgtgtcc atcacagcgg taaacggcat
    20461 atcgagaatc gccagcgact tagtgcccca gttggtttca tcgtcgccaa tcatggttgc
    20521 gctggcgcgg gtgccgagat acgttccttc tttccctccg gtatgggaca tcacgctgga
    20581 gcatcctccg agtaatgtca ttccgctgca tatcatgaac gctaacaaca catttcttat
    20641 cataataaat tcatctgttg atcgtgggtg ttggcctgat gagttatagc gatcccttgc
    20701 tgaaaataac atcatcatta cgtcgcactg tggcggctat cgcactttaa cgtttcgtgc
    20761 tgccccctca gtctatgcaa tagaccataa actgcaaaaa aaagtccgct gataaggctt
    20821 gaaaagttca tttccagacc catttttaca tcgtagccga tgaggacgcg cctgatgggt
    20881 gttctggcta cctgacctgt ccattgtgga aggtcttaca ttctcgctga tttcaggagc
    20941 tattgattat gcgtaacttt gatttatccc cgctttaccg ttctgctatt ggatttgacc
    21001 gtttgtttaa ccacttagaa aacaaccaga gccagagtaa tggcggctac cctccgtata
    21061 acgttgaact ggtagacgaa aaccattacc gcattgctat cgctgtggct ggttttgctg
    21121 agagcgaact ggaaattacc gcccaggata atctgctggt ggtgaaaggt gctcacgccg
    21181 acgaacaaaa agagcgcacc tatctgtacc agggcatcgc tgaacgcaac tttgaacgca
    21241 aattccagtt agctgagaac attcatgttc gtggcgctaa cctggtaaat ggtttgctgt
    21301 atatcgatct cgaacgcgtg attccggaag cgaaaaaacc gcgccgtatc gaaatcaact
    21361 aattccttaa ggccgcctgg cgcggcctga catctccatg ctcgccgtca gggagcatat
    21421 gcgaatcttc ggatttgcag gtacttactc gcttcttaga aggagaaatg actatgcgta
    21481 acttcgattt atccccactg atgcgtcaat ggatcggttt tgacaaactg gccaacgcac
    21541 tgcaaaacgc cggtgaaagc cagagcttcc cgccgtacaa cattgagaaa agcgacgata
    21601 accactaccg cattaccctt gcgctggcag gtttccgtca ggaagattta gagattcaac
    21661 tggaaggtac gcgcctgagc gtaaaaggca cgccggagca gccaaaagaa gagaaaaaat
    21721 ggctgcatca agggcttatg aatcagccat ttagcctgag ctttacgctg gctgaaaata
    21781 tggaagtctc tggcgcaacc ttcgtaaacg gtttactgca tattgattta attcgtaatg
    21841 agcctgaacc catcgcagcg cagcgtatcg ctatcagcga acgtcccgcg ttaaatagct
    21901 aactagctat tctctttgcc ccgccattct ggtggggctt tttttgtctt acttactcaa
    21961 caatgcgtta aatttcgact gtttaagata tttcggcacg ttctttcccg tgataatgtg
    22021 cgctataagg caaatctgct tcaccgattc agccataatc cgtagcaatt aaaaatgtta
    22081 tctcaagaga aatggacgat gagtgatata gcattaacgg tcagtattct ggctttggtg
    22141 gcagtcgtcg gtttgtttat cggcaatgtc aaatttcgcg gcataggatt aggtattggc
    22201 ggcgtgctgt ttggtgggat catcgtcggc cattttgttt ctcaggcggg gatgacatta
    22261 agtagcgata tgctgcatgt tattcaggaa tttggcctga tcctgttcgt ttatactatc
    22321 gggattcagg tagggccggg cttctttgcc tcattgcgcg tctccggatt acgcctcaac
    22381 ctgtttgctg ttctgatcgt catcatcggt ggtctggtta ccgccatcct gcataaactg
    22441 tttgatattc cactgccggt agtgctgggg attttctccg gtgcggttac caatacgcca
    22501 gcgctggggg cagggcagca gattttgcgc gacctgggta caccaatgga aatggtcgat
    22561 cagatgggga tgagttacgc gatggcgtat ccattcggca tttgcgggat tttgttcacc
    22621 atgtggatgt tgcgggttat tttccgcgtc aatgtcgaga cagaagctca gcagcacgag
    22681 tcttcacgca ccaatggcgg cgcgctgatc aagactatca atattcgcgt tgagaaccct
    22741 aacctgcatg atttagccat taaagatgta ccgattctca acggcgacaa aattatctgc
    22801 tcgcgtctga aacgcgaaga aaccctaaaa gttccttcgc cagataccat tattcaactg
    22861 ggcgatttgc tgcatctggt gggtcagcca gcggatttac ataatgcgca actggtgatt
    22921 ggtcaggagg tcgatacttc gctgtccacg aaaggcactg atttgcgcgt cgagcgtgtg
    22981 gtggtcacca atgaaaacgt gctcggaaaa cgtattcgcg acctgcactt taaagaacgc
    23041 tatgacgttg ttatctcgcg cctgaaccgt gccggggtcg aactggtcgc cagtggcgat
    23101 atcagcctgc agttcggcga tatcctcaac ctggtggggc gtccgtccgc aattgatgcc
    23161 gttgccaatg tgctggggaa tgcgcagcaa aaactgcaac aggttcagat gctgccagtg
    23221 tttattggca tcgggcttgg cgtattgtta ggttctattc ccgtctttgt gccaggattc
    23281 ccggccgcgt tgaaactggg gctggcgggc ggtccgctga ttatggcgtt gatcctcggg
    23341 cgtatcggca gtatcggcaa gctgtactgg tttatgccgc caagtgccaa cctcgcgctg
    23401 cgggagctgg ggatcgtgct gttcctctcg gtcgttggtc tgaaatctgg tggggatttt
    23461 gtgaataccc tggtcaatgg cgaagggcta agctggattg gttatggtgc cctgatcacc
    23521 gccgttccgc tgattactgt tggcattctg gcgcggatgt tagccaaaat gaattacctg
    23581 accatgtgcg ggatgctggc aggttccatg accgatcctc cggcgctggc gtttgctaat
    23641 aatcttcatc caaccagcgg tgcggcggcg ctctcttacg ccactgtcta tccgttagtg
    23701 atgttcctgc gcattatcac cccccaatta ctggcggtgc tcttctggag tatcggttaa
    23761 ctcttttccg ggtggagtcg ccgtaaatgg tactccacct ggtattcgct ggcatttctg
    23821 aacatcactg aataattcaa aaactccccg ctctcgctat atgacaacga ggtgatccgc
    23881 agcagtggcg tgtgttccgc taccttcatt tgccgcgcca gcagtctatc ggcgagcact
    23941 ggcgtcaggc tttcataatt accgccaatc aaaatcccgc actcttgttc aatatattca
    24001 aacttcgacc cttccagatg ttgcagcgaa agattacgga acagttttac cggcatatag
    24061 ctgtcttcca gcatcagcgg tttcccttcc acaaaacgaa cgcgacggga gaagtagatc
    24121 tgctcgttga tttgaatccg taactggctg gcaatggccg gaggcgcagg catgatttca
    24181 aaaatcagca cctggctggt gaccgttttt ccctgccgtt ttaacacctc caccagcccg
    24241 gtcaggctgg cggtttgatg cagtacatct ttgcgcacca ggtaagtgcc gctgccgtgg
    24301 cggcggacca ccaatcccca cgctaccagc aggtcaatgg ctttacggat ggtcatccgc
    24361 gataccgcaa attcttccgc caactttttt tcaccgggaa gaaggctatt tagcgtgaaa
    24421 tctgcggaat taagtcgaat tcttaaccgc tccgcaatgc ttttgtagat catctctaga
    24481 tctctttttt gcctgcaagc acgggtaatg cgcccacaag ttctgcttaa tcgattgaaa
    24541 aaacatcttt tttttaaaga tgtgttcgat gctgtgtacc tgtgctcaca gatgtctact
    24601 ttttcgcgaa aacgtagatc tctaccgccc aacgaaaagc atgaaagcga tcacgaatcc
    24661 cattgggtcg tcatgttctg ccgatcgcat cttcctatcc tcgctccagg cctgccgcat
    24721 aaccaatcag gcttcctact tacagaattg agaaaagagg atgtggaaat gctcagtcaa
    24781 attcaacgct ttggcggcgc gatgttcacg ccagtgctgc tgtttccctt cgccgggatt
    24841 gtggtgggtc ttgccatctt gctgcaaaac ccgatgtttg tcggggaatc actgaccgat
    24901 ccgaacagtt tattcgcgca aatcgtacac attattgaag agggcggttg gacggtgttc
    24961 cgtaatatgc cgctgatttt tgctgtcggt ttacccattg gccttgctaa gcaagcgcag
    25021 gggcgtgctt gtctggcggt gatggtgagt ttcctgacct ggaactattt catcaacgcg
    25081 atgggaatga cctggggaag ctacttcggc gtcgatttca ctcaggacgc ggtggcaggt
    25141 agcggtctga caatgatggc cgggattaaa accctcgata ccagcattat cggcgcaatt
    25201 atcatttccg gcattgtgac ggcgttgcat aaccgtctgt tcgataaaaa actgccggtt
    25261 tttctcggca ttttccaggg gacgtcttat gtggtgatta tcgccttcct ggtgatgatc
    25321 ccctgtgcct ggctgacgtt gctcggctgg ccaaaagtac aaatggggat tgaatctctg
    25381 caagcgttcc tgcgttcggc gggtgcgctt ggggtgtggg tttacacctt cctcgaacgt
    25441 attctgatcc caaccggttt acaccacttc atctacggac agtttatctt tggtccggca
    25501 gctgttgaag gcggcattca gatgtactgg gcgcagcatc tgcaagagtt cagtctgagc
    25561 gccgagccgc tgaaatcgtt gttcccggaa ggcggttttg ccctgcacgg taactcaaaa
    25621 atctttggtg ccgtgggcat ttctttagcg atgtacttca ctgccgcacc ggaaaatcgg
    25681 gtaaaagtgg cgggcttgct gattcccgca accttaaccg ccatgctggt gggaattacc
    25741 gaaccgctgg aatttacctt cctgttcatt tcaccgttgc tgtttgcggt acacgctgtg
    25801 cttgcggcct caatgtcgac cgtgatgtat ctctttggtg tggtgggcaa catgggcgga
    25861 ggtctgattg accaggtttt accgcaaaac tggatcccga tgttcagcaa ccacgcggat
    25921 atgatgctga cccaaatcgc cattgggttg tgctttaccc tgctgtactt cgtggttttc
    25981 cgcacactga ttctgcagtt caacatgtgc acgccgggac gtgaagatgc ggaagtgaaa
    26041 ctctactcaa aagccgaata caaagcctcg cgaggccaaa ccaccgcggc agagccaaaa
    26101 aaagagctgg atcaggctgc cggtatcctg caagccctgg gcggggtcgg caatatctcc
    26161 agcattaaca attgcgcgac gcgtttacgc attgcactgc atgacatgtc acaaacgctg
    26221 gatgacgaag tctttaaaaa gctgggagcg cacggcgtct tccgtagtgg cgatgccatt
    26281 caggtgatca ttggtctgca tgtatcccag ctgcgtgaac agctcgatag cttaattaat
    26341 tctcatcaat cagcagaaaa tgttgccatt acggaggcag tataatgacc aaattctcag
    26401 tggttgtcgc aggcggtgga agcaccttta cgccaggcat cgtgttgatg ctcctggcga
    26461 atcaggagcg tttcccgctt cgtgcactga aattttatga taacgatggc gcgcggcagg
    26521 aagtgattgc cgaagcctgt aaagtcatcc ttaaagagaa agcgccggac attgcgttta
    26581 gttacaccac cgatcctgaa gtggcgttca gcgacgttga ttttgtcatg gcgcacatcc
    26641 gcgtcggcaa gtacccgatg cgcgaactgg atgaaaaaat cccactgcgc cacggtgttg
    26701 ttggtcagga aacttgcgga cccggcggaa tagcgtacgg catgcgttcc attggcggcg
    26761 tcctggaact ggtggattat atggaaaaat attcaccaaa tgcctggatg ctcaactact
    26821 ccaacccggc agccattgtc gcagaagcca cgcgtcgtct gcgcccgaat gcgaaaatcc
    26881 tcaacatctg tgacatgcca atcggtattg aaagccggat ggcgcaaatt gttgggctgc
    26941 aagatcgcaa acagatgcgc gtgcgctact acggcctgaa ccactggtgg tcggcgatat
    27001 cccgcagttt cagaaagggt taatgagtca gcaagtggcg gtggaaaaac tggtcgtgga
    27061 cgcctgggaa cagcgttcat atcagcacct gtggcaggcg attacgttgt cgaaaacggt
    27121 accgagcgcc tcggtcgcca aagctattct ggatgagttg ctggaggcca acaaagcgta
    27181 ctggccagag ttacgttaat tgtgtggact ggcatccgtt gggtgctggt ccgtattctt
    27241 tagcacggct gacggcgcat aaccaaattt atgtttaaag gctttgctga aatgaaagga
    27301 gtcgaagaag ttgagcgtat cagcgacttc gccaaccgag tttccttgtt gctttaatag
    27361 cgatagcgcc agatccagac gcgcatcgag gtaatattct ttcggcgtct tgccggtata
    27421 gcgtaaaaac agacgacgca accaggcttc gctacagggg atcgtggcag ccatatcagc
    27481 tacgctccag cgttgttgca gactggcatg taaagtggca attaattttt caatttgccg
    27541 ccgttgtgga tcttttttac catctgcgta cataagacaa atccactgat aaataatttt
    27601 cgtcagaaaa gcgactgcca gattattttt tattgcttct ggtgaagtta ttaactcagc
    27661 aacttcggtg agttcctgat tataaatttc gccattataa ataacgcttt gctgaccaac
    27721 gggaatatcc atcatactgg tgggggtaaa ttccatccag tactgttccc agactaaacc
    27781 ttcacagtga taagagtgaa tatccattgg ctttaagaat ataatacagt tgccatgcag
    27841 tgttatttgt tcgccattct ttaaaagaat tttcccacat ccttgtacgg tatacaccgc
    27901 aacccatgag ttggcgataa gcggtttctt cttgtcccgc ggccagatca cccggtaatg
    27961 ctcgtcggcg taggtatgcc agagcgagat cagagaaatt ccgctggaga tcacattttc
    28021 gttaatcaat aagggaatat tgtacggagt ttcgtttttt acatctgaac tttgcaattt
    28081 tccattcatt ttaatatccc tccggcgaat tatgctcaca acctgaactt aacagaaaac
    28141 aatccgttca gggatacgac gtttgtattt taagaatctg actgcctgac ccgacgcatt
    28201 ttacctctcc ctatattcat gcgtccggga cataaacata aataagggct atgagatgaa
    28261 ttcgttacaa atcttgagtt ttgtcggttt tacgctgctg gtggcggtga tcacctggtg
    28321 gaaggtccgc aaaacagata ccggatcgca acaaggctat tttcttgccg gacgttcact
    28381 aaaagcgccg gttattgccg cttcgttaat gctaaccaac ctttccacgg aacaactggt
    28441 cggcctttcc gggcaggcct acaaaagcgg catgtcggtg atgggctggg aagtgacttc
    28501 agcggtgacg ctgatcttcc tcgcgctaat ctttttaccg cgctatctga agcgcggcat
    28561 cgccaccatc cccgattttc tggaggaacg ttatgataaa acgacgcgta ttatcatcga
    28621 cttctgcttc ctcattgcca ccggcgtctg ctttctgccg attgttctct actccggcgc
    28681 gttggcgctc aacagcctgt ttcacgtcgg ggaatcgcta cagatttctc acggtgcggc
    28741 tatctggcta ctggtaattt tgcttggtct ggcgggaatt ttgtatgcgg tgatcggcgg
    28801 actgcgcgca atggcagtgg cggactccat caacggtatt gggctggtta tcggcgggtt
    28861 gatggtgccg gtatttggcc tgatcgcgat gggcaagggc agctttatgc agggcattga
    28921 gcaactcacc accgttcacg ccgagaaatt aaactcaatc ggtggcccga ccgatccctt
    28981 gccgattggc gcggcattta ccggtttgat tctggtgaac accttttact ggtgtacaaa
    29041 tcagggcatc gtgcaacgca cgctggcgtc aaaaagcctg gcggaagggc aaaagggggc
    29101 gctgttaacg gcggtgctga aaatgctcga cccgctggta ctggtgctgc cagggttgat
    29161 tgcgtttcat ctgtatcagg atttaccgaa agccgacatg gcctacccga cgctggtcaa
    29221 taacgttctg ccagtgccaa tggtgggttt cttcggcgcg gtgttatttg gtgcggtgat
    29281 cagtaccttc aacggctttc tgaatagcgc cagtacgtta ttcagtatgg gtatttaccg
    29341 tcgcatcatt aaccagaatg ccgagccgca gcagctggtc accgtcgggc gcaaatttgg
    29401 tttctttatc gctatcgttt cggtgctggt cgcgccgtgg atcgccaacg cgccgcaggg
    29461 gctgtatagc tggatgaaac agctcaacgg catttacaac gtgccgctgg ttaccatcat
    29521 cattatgggc tttttcttcc cgcgcatccc ggcgctggcg gcaaaagtgg cgatggggat
    29581 tggcataatc agctacatca ccatcaacta tctggtgaag ttcgacttcc atttcctcta
    29641 tgtgctggcc tgtacgttct gcatcaacgt ggtcgtgatg ctggtgatcg gttttatcaa
    29701 accgcgcgcc acgccgttca ccttcaaaga tgcgtttgcg gtggacatga aaccgtggag
    29761 aaacgtcaag atcgcgtcaa ttggcatcct gttcgcgatg attggcgtct atgccgggct
    29821 ggctgaattc ggcggctacg gtacgcgctg gttagcgatg atcagttatt tcattgccgc
    29881 cgtagtgatt gtctacctga tttttgacag ctggcggcat cgtcacgacc cagccgtaac
    29941 ctttactccc gacgcgaagg atagcctatg aaacgcccca attttctgtt catcatgacc
    30001 gatacccagg ccaccaatat ggtcggttgc tatagcggta aaccgctgaa tacgcaaaat
    30061 attgatagtc tggcggcgga aggtattcgc tttaattccg cctacacctg ttcaccggtt
    30121 tgtacgcctg cacgcgccgg actatttacc ggtatctacg ctaaccagtc cggcccgtgg
    30181 accaacaacg tcgcgccagg caaaaacatc tccactatgg ggcgctactt taaggatgcc
    30241 ggctatcaca cctgttacat cggcaaatgg catctcgacg gtcatgacta tttcggcact
    30301 ggcgagtgtc cgctggagtg ggacgctgat tactggttcg atggggcgaa ctaccttagc
    30361 gaactgacgg aaaaagagat cagcctgtgg cgcaatggcc taaacagcgt tgaggattta
    30421 caggcgaacc atatcgacga aaccttcacc tgggcgcacc gcatcagcaa tcgggcggta
    30481 gattttctgc aacagcccgc gcgcgccgag gaacccttcc tgatggtggt ttcgtatgat
    30541 gagccgcatc acccgttcac ctgtccggtg gagtatttag agaaatacgc tgatttttac
    30601 tacgatctgg gcgagaaagc tcaggatgac ctggcgaaca aaccggaaca tcaccgctta
    30661 tgggcgcagg cgatgccatc gccagtcggt gatgacgggc tttatcacca tccgctctat
    30721 tttgcctgta atgactttgt tgatgaccaa atcggacggg tcatcaatgc cttaacgcca
    30781 gagcaacgtg aaaatacgtg ggtcatttat acttccgatc acggcgaaat gatgggcgca
    30841 cataagctga tcagtaaagg agcggcgatg tatgacgaca tcacccgtat tccgctgatc
    30901 atccgttcgc cgcaagggga gcggcgacag gtcgatacgc cagtcagtca tatcgattta
    30961 ctgccgacaa tgatggcgct ggcagatatt gaaaaaccag agattctgcc gggggaaaat
    31021 atccttgccg tgaaagagcc acgcggtgta atggtggaat ttaaccgcta cgagattgag
    31081 catgacagct ttggcggttt tattccggtg cgttgctggg tgacggatga ctttaaactg
    31141 gtactcaacc tcttcaccag tgatgaactt tacgatcgcc gtaatgaccc aaatgaaatg
    31201 cataacctga tcgatgatat ccgttttgca gacgttcgca gcaaaatgca tgacgcctta
    31261 ttggattaca tggacaaaat tcgcgatccg ttccgcagtt accaatggag cctgcgtccg
    31321 tggcgtaaag atgcactgcc gcgctggatg ggggcatttc gtccacgccc acaagatggc
    31381 tattcgccgg tggtacgtga ctatgacacc ggcctaccga cgcaaggagt gaaagtggaa
    31441 gagaaaaaac agaagttctg acgataatgc ccgctgatga acacccggcg ggcattattc
    31501 aggctgctta aaccagtgaa ccgccggtca ggaaggaaaa aatcaggaat atcagcaaaa
    31561 tgctgcacac gatgccgata ccaaaaagca aggtatggaa agccagcgtc ccgccgccaa
    31621 aaaccagcgc gccgcgaatg ccccagcgat tttccttacg cataccgcca ataaatgcgc
    31681 aaagtagcgc agcaatggcc agtgcgatac cgctttgatc gaggatttta tcgacatcta
    31741 cgtcctcttt tttctccacg gtggtaattt tttcgccttt tattccggcg agaagacctc
    31801 ttttaatttc agccgttttg tccgccacga tgctttcaaa cgttggcggt ggtgcggaaa
    31861 aagggccgaa tgaaaagtgg ataatcccca tcattaaagc gatggcaccg aacagcatgc
    31921 ccaatgaact gattttattt tgagctatga tacccatctg acctccttgt agcggatggc
    31981 gaaagcatat caggaaacgg gcggacgaaa ttcttaaaaa aaaaccgcta aaggtagagc
    32041 gttagtgaaa ttgcaaagga gcaacaatga aaatttcccg ccttggagaa gcgcccgatt
    32101 accgcttctc gctggctaat gagcgtactt ttctggcgtg gatccgtacc gcactaggat
    32161 ttctggcggc gggggttggg cttgatcaac ttgcgccaga tttcgccacg cccgtcattc
    32221 gtgaactgct ggcgttgttg ttgtgtctgt tttccggcgg gttagcgatg tatggctacc
    32281 tgcgctggtt acgcaatgaa aaagcgatgc gtctgaaaga ggacctgccc tacaccaaca
    32341 gcttattaat cattagctta atattgatgg tcgtggcggt gatcgttatg ggactggtgt
    32401 tgtatgccgg atagccgcaa agccagacgc attgccgacc caggactgca gccggagcgc
    32461 acatcactgg cgtggtttcg taccatgctg ggctacggcg cgttgatggc gttggctatc
    32521 aaacacaact ggcaccaggc gggcatgtta ttctggattt cgattggcat tctcgccatc
    32581 gtggcgctga tcctctggca ctacacccgt aatcgcaatt taatggatgt cacgaatagc
    32641 gatttttccc aatttcacgt agtccgtgac aaatttttga tctccctcgc ggtgttatct
    32701 ctcgcaatac tgtttgctgt aacgcatata catcaactta tcgtatttat tgagagagtc
    32761 gcatgacagg aagtcaggtc attgatgctg aagaggacag gcataaactg gttgtagagt
    32821 acaaggatgc tctgcaacct gccgattttt atcacaattt caaacagcgg ggcatccgct
    32881 ccgtacagct cattccctat cttgaatttg atgaccgggg cgatctgacg gccgcttcgg
    32941 tgacagcaga gctgtggggg aaatttttaa ttgccctgtt cgagtgttgg gtgcgggcag
    33001 atatcagtcg tatctcgatt gagctttttg acgccacctt acaaaaatgg tgtggcagcg
    33061 aaaacccgca accgcgccgt gactgccagg cgtgcgactg gcatcgcctg tgcccgcacg
    33121 ctcgccagga gacgccagac agcgtgcttt gtgcgggcta tcaggcgttt tattcttact
    33181 ccgcaccgca tatgcgggtg atgcgtgatc tgataaagca gcatcgttct cccatggagc
    33241 tgatgacgat gctgcggtga cgtgcgctta aacgggctgc ccctgatgcg acatccgcgt
    33301 cgccagcggc agccagcaca gcacgatcaa caatcccatt aaggtcatca acaaccccag
    33361 gctgccctga ccggtttgcg gcaacatcgc agagagcgac gccagcacgc cggaaccaat
    33421 gttttgcaga ccgccgacca gcgcgccagc cgtgcccgcc aggaagggga acggctccat
    33481 cgcgccgctg gtcgccagcg gaaacagcat cccggcaccg aaaaagaaca gcgcggcggg
    33541 aacgagcagc gtccagacat tcatcacgcc aaaccagtcg gggatccaca tcagcaagcc
    33601 agccagcagg cagcagataa ccgactgcca cattaacgtg gagaagcgtt tattgggacg
    33661 tccggcaaac catgcgccaa aaaacgctgc cggaatcggc agaataaaca aaatactgac
    33721 cgtcatactg ctcagcccta acaccgcgcc catcagcacg ccggagcagg cttcaaaggc
    33781 ggcaatcccg gccagaccgc caatcagcat cagcaaataa cagttaaaac cgctgttacc
    33841 gaaaagcgtt ttataactgg taagcaggcg cgtgcgcggt gcatcgaccg gacgcgtttc
    33901 cggcatccag cgggccatac tgaaggtcac accagcacaa agaaccaaca aaaagagata
    33961 acaggcgcgc cagttccaca tcgtatccag cagaccgccg attagcggtg cgagcaacgg
    34021 actgacgaga atccccatgt ttaacaggct gttagcatgg cgcaactgtg tccgttcata
    34081 taaatctcgc ggcaaagtac gcgccattac gccgccaacg ccggtgccca tcccctgcat
    34141 cgcgctggcg gcaatcaaca ccgtcaaact ggaggtcgtg accgcgacca gcgttgccag
    34201 cataaaaatg gacattccga cgaggatcac cggtcggcgg cccacgcggt cggaaatcgg
    34261 gccataaaac agctgtgaga caccgtaagt cagcagataa gcgcccatta cgctctgcac
    34321 cgccccttca cggacgttga gatcgcgcgc catatcggca atagctggaa tataaatggt
    34381 ttgcgccatc tgaccgacgg ccacgagtaa taccaacatc aataacaaat tgacgtttct
    34441 ttgccttttc attatcacgg atgcttttat aaaaataatt agaataagtc actacgcatt
    34501 cccataaatg cgggagagga atctatcaca ttgatgaatg ttagccagat atacgccagg
    34561 aatggggaat tgtttagcgg cagaatatgt aaacaaaagc ggcaataaat gttgccggga
    34621 gggattacgt gcgaactgta accctctccc caaaggggcg agggggaaac caccgttccc
    34681 tttcagaaaa tgagctaaac attgcacggg acggtcccct cgcccctttg gggagagggc
    34741 tagggtgagg ggcacaggca gcgcaggtgc aaacattctg ctggtagaag aagcccccaa
    34801 ctccaacccg cacgctaaat ataaaagggg agcggttacc cgctcccctt tggtgcgact
    34861 tgaatctgaa ttacttcagg tatttcagaa cagcatcaag cagttgcagt gctgcaacaa
    34921 tgagtttgag gataagaatg gcgatatcca ccaggctcat acgcgtctcc tgtggttcag
    34981 gagcactgcc agctgacgta cctttccgct gcctgttgcc agctgttgtg cacgcggtat
    35041 cttaaggaga aacgttgtgc ttgttgacat aacacagtgt gctcacgcgg ggttaaggcg
    35101 ctgacggcac cacccgtttc agccaggact tagtgcgccg ggtaacgatg cggcccccgc
    35161 ataacacatt gcgtacagtg atattataga agtttactgt ataaataaac agtaatattt
    35221 ggacaaaacg caaactgtga tcgatactca actatgccct aaatacgtca aaattcgtgc
    35281 cgaaattgcg cgttctgcgc ggaacacgta tactttcagt gttgacataa tacagtgtgc
    35341 tttgcggtta ccagccgcag gcggctgatg aaacctcgct ccggcggggt tttttgttac
    35401 ctgcaattca gtacaaaacg tgatcagccc ctcaattttc ccttgctgaa aaattttcca
    35461 ttgtctcccc tgtaaagctg tgcttgtata aatattgtta aacacaaaac caacaaggtc
    35521 cccaatgact acttccatgt tcaacgcaaa actactacca actgcgctat ccgccgcagt
    35581 ggtcgtcgtg cgtgtggtgg tggtcgtcgg caatgcgccg tagggactgg aacaacacac
    35641 gattccaaaa ccccgccggc gcaaaccggg cggggttttt cgtttaagca cctcccggaa
    35701 agtcggccca gaagaaaagg actggagcat ggcaagttcg ggcacaacat cgacgcgtaa
    35761 gcgctttacc ggcgcagaat ttatcgttca tttcctggaa cagcagggca ttaagattgt
    35821 gacgggcatt ccgggcggtt ctatcctgcc tgtttacgat gccttaagcc aaagcacgca
    35881 aatccgccat attctggccc gtcatgaaca gggcgcggga tttatcgctc aggggatggc
    35941 gcgcaccgac ggtaaaccgg cggtctgtat ggcctgtagc ggaccgggtg cgactaacct
    36001 ggtgaccgcc attgccgatg cgcggctgga ctccatcccg ctgatttgca tcaccggtca
    36061 ggttcccgcc tcgatgatcg gcaccgacgc cttccaggaa gtggacacct acggcatctc
    36121 tatccccatc accaaacaca actatctggt cagacatatc gaagaactcc cgcaggtcat
    36181 gagcgatgcc ttccgcattg cgcaatcagg ccgcccaggc ccggtgtgga tagacattcc
    36241 taaggatgtg caaacggcag tttttgagat tgaaacacag cccgctatgg cagaaaaagc
    36301 cgccgccccc gcctttagcg aagaaagcat tcgtgacgca gcggcgatga ttaacgctgc
    36361 caaacgcccg gtgctttatc tgggcggcgg tgtgatcaat gcgcccgcac gggtgcgtga
    36421 actggcggag aaagcgcaac tgcctaccac catgacttta atggcgctgg gcatgttgcc
    36481 aaaagcgcat ccgttgtcgc tgggtatgct ggggatgcac ggcgtgcgca gcaccaacta
    36541 tattttgcag gaggcggatt tgttgatagt gctcggtgcg cgttttgatg accgggcgat
    36601 tggcaaaacc gagcagttct gtccgaatgc caaaattatt catgtcgata tcgatcgcgc
    36661 agagctgggt aaaatcaagc agccgcatgt ggcgattcag gcggatgttg atgacgtgct
    36721 ggcgcaattg atcccgcagg tggaagcgca accgcgtgca gagtggcacc agttggtagc
    36781 ggatttgcag cgcgaattcc cgtgtccaat cccgaaagcg tgcgatccat taagccatta
    36841 cggcctgatc aacgccgttg ccgcctgtgt cgatgacaat gcgattatca ccaccgatgt
    36901 gggtcagcat cagatgtgga ccgcgcaagc ttatccgctc aatcgcccac gccagtggct
    36961 gacctccggt gggctgggca cgatgggttt tggcctgcct gcggcgattg gcgcggcgct
    37021 ggcgaacccg gatcgcaaag tgttgtgttt ctccggcgac ggcagcctga tgatgaatat
    37081 tcaggagatg gcgaccgcca gtgaaaatca gctggatgtc aaaatcattc tgatgaacaa
    37141 cgaagcgctg gggctggtgc atcagcaaca gagtctgttc tacgagcaag gcgtttttgc
    37201 cgccacctat ccgggaaaaa tcaactttat gcagattgcc gccggattcg gcctcgaaac
    37261 ctgtgatttg aataacgaag ccgatccgca ggctgcattg caggaaatca tcaatcgccc
    37321 tggcccggcg ctgatccatg tgcgcattga tgccgaagaa aaagtttacc cgatggtgcc
    37381 gccaggtgcg gcgaatactg aaatggtggg ggaataagcc atgcaaaaca caactcatga
    37441 caacgtgatt ctggagctca ccgttcgcaa ccatccgggc gtaatgaccc acgtttgtgg
    37501 cctttttgcc cgccgcgctt ttaacgttga aggcattctt tgtctgccga ttcaggacag
    37561 cgacaaaagc catatctggc tactggtcaa tgacgaccag cgtctggagc agatgataag
    37621 ccaaatcgat aagctggaag atgtggtgaa agtgcagcgt aatcagtccg atccgactat
    37681 gtttaacaag attgcggtgt tttttcagta accgctcaag gcttgaacaa catcgcgctt
    37741 atcgttaagg taagcgcgtt tttttttacc cgccaggaca agaccatgat caccgttgcc
    37801 cttatagacg atcacctcat cgtccgctcc ggctttgcgc agctgctggg gctggaacct
    37861 gatttgcagg tagttgccga gtttggttcg gggcgcgagg cgctggcggg gctgccgggg
    37921 cgcggtgtgc aggtgtgtat ttgcgatatc tccatgcccg atatctccgg tctggaactg
    37981 ctaagccagc tgccgaaagg tatggcgaca ataatgctct ctgttcatga cagtccggcg
    38041 ctggttgagc aggcgcttaa cgcgggggca cgcggctttc tctccaaacg ctgtagccct
    38101 gacgaactga ttgcggcggt gcatacggtt gccacgggcg gctgttatct gacgccggat
    38161 attgccatta aactggcatc cggtcgtcag gacccgctaa ccaaacgcga acggcaggtg
    38221 gcggaaaaac tggcgcaagg aatggcggtg aaagagattg ctgccgaact gggcttgtcg
    38281 ccgaaaacgg tgcacgtcca tcgcgccaac ctgatggaaa aactgggtgt cagtaacgac
    38341 gtagagctgg cgcgccgcat gtttgatggc tggtgatgaa gacgttgttc tcccgcttaa
    38401 ttaccgttat tgcctgcttt tttatcttct ctgccgcatg gttttgcctg tggagtatca
    38461 gcctgcacct ggttgagcgc cctgatatgg cggtgctgtt atttccgttt ggtctgcgtc
    38521 tggggctaat gctgcaatgc ccgcgcggat actggccggt gctgctgggc gcggagtggc
    38581 tgctgattta ctggctaacg caggcggtcg gtttaaccca ttttccgtta ttgatgatcg
    38641 gtagtttact gacgttactg cccgtggcgc tgatctcgcg ctatcgccat cagcgtgact
    38701 ggcgcacctt gctgttacag ggtgcggcgt taacggcggc ggcgttgttg cagtcgctgc
    38761 cctggctttg gcacggcaaa gagtcgtgga atgcgctgtt gctgacttta actggcggcc
    38821 tgacgctggc cccgatatgt ctggtgttct ggcactatct cgccaataac acctggctgc
    38881 cgctcggccc gtcactggtt tctcagccga tcaactggcg cgggcgacat ctggtctggt
    38941 acttgctgct gtttgttatc agtctctggc tccagttggg attgccggac gaactgtcgc
    39001 gctttacgcc attttgtctg gcgctgccga ttatcgcgct ggcctggcac tatggttggc
    39061 aaggggcgct gattgcgacg ttgatgaacg ccatcgcgct gatcgccagt caaacctggc
    39121 gcgatcatcc ggtggattta ttgctctcgc tgctggtgca aagtctgaca gggttgttgc
    39181 ttggcgctgg catccagcgg ttgcgtgaac ttaaccagtc gctgcaaaag gaactggcgc
    39241 gcaatcagca tctggctgaa cggttgctgg aaaccgaaga gagcgtgcgc cgtgatgtgg
    39301 cgcgtgagct gcatgatgat atcggtcaga ccatcacagc cattcgtact caggcgggca
    39361 ttgttcagcg gctggcggca gataacgcca gcgtgaagca gagcgggcag ctcatcgaac
    39421 aactgtcgct gggcgtgtac gacgcggtgc gccgtttatt aggacggtta cgtccgcgcc
    39481 agttggatga tctctccctg gagcaggcca tccgctcact gatgcgggaa atggagctgg
    39541 aagggcgcgg cattgtcagc catctcgaat ggcgaatcga tgaatcagca ttaagtgaaa
    39601 accagcgcgt gacgctgttt cgtgtctgcc aggaagggct gaacaacatt gtgaaacatg
    39661 ctgatgccag cgcggtcacg ctgcaaggct ggcagcagga tgaacggttg atgctggtga
    39721 ttgaagacga tggcagcggt ttgccgccgg gttccgggca acaaggtttt ggcctcaccg
    39781 gaatgcgcga gcgcgtaacg gcgctgggcg gcacattaca catttcctgt ctgcacggca
    39841 cgcgtgtcag cgtttctcta cctcaacgct atgtctaagg ttcgatgatg ttgccgtttc
    39901 tgaaagcgcc tgccgatgcg ccattaatga ctgacaaaca tgaaattgat gcccgctatc
    39961 gctactggcg tcggcatatt ctgctgacca tctggctggg ttacgcgctg ttttacttca
    40021 cgcgtaaaag ttttaacgcc gccgtaccag aaatccttgc taacggcgtg ctcagccgta
    40081 gcgatatcgg cctgttagcg accctgtttt acattaccta cggcgtgtcg aagtttgtct
    40141 ccggcattgt cagcgatcgc tcaaatgccc gttattttat ggggataggg cttatcgcca
    40201 cgggcatcat caacattctg tttggcttct cgacgtcgct atgggcgttt gccgtgctct
    40261 gggtgctgaa cgcctttttc cagggctggg gttcaccggt gtgtgcgcgt ctgttaacgg
    40321 cctggtattc acgtaccgag cgcggcggtt ggtgggcatt atggaacacg gcgcataacg
    40381 tcggcggcgc actcattccc attgtgatgg cagcggctgc gctgcattac ggctggcgtg
    40441 ccgggatgat gattgctggt tgtatggcga tagtcgtggg gatttttctc tgctggcggc
    40501 tacgcgatcg cccgcaggcg ttaggtttac cggcggtcgg tgaatggcga cacgacgcgc
    40561 tggaaattgc tcaacaacaa gaaggggcag ggttgacgcg taaagagatc ctcaccaaat
    40621 atgtgttgct gaatccgtat atctggctgc tttcgttttg ctatgtgctg gtctatgtgg
    40681 tccgggcggc gatcaacgac tggggcaatt tgtatatgtc cgagacactg ggcgtcgatc
    40741 tggtcacggc gaatacggca gtgacgatgt ttgaactggg cggatttatc ggtgcgctgg
    40801 tagccggttg gggctcggac aaattgttta acggcaaccg tgggccgatg aatttgattt
    40861 tcgccgccgg aattttgctt tcagtcggct cgctgtggct gatgccattt gccagctacg
    40921 tgatgcaggc aacctgcttc ttcaccattg gtttttttgt ctttggcccg cagatgttaa
    40981 tcggcatggc ggcggcagag tgttcccaca aagaggcggc aggggcggcg acggggtttg
    41041 tcggcttgtt tgcttatctg ggggcgtcgc ttgctggttg gccgctggcg aaagtactcg
    41101 atacctggca ctggagcgga ttttttgtgg ttatctctat cgccgccggg atttccgcac
    41161 tgctgttact gccctttttg aacgcccagg caccgcgcga agcgtgatgc atctcacctt
    41221 ttcacttcat atccggcaaa actaagaaat tttccaggtt ttgcctggac gctatctcag
    41281 gcctgatttg ctgctgattt ttacaatgca tgcctcacgc aggtattcat ttcaggagta
    41341 acccatgctg gctttcttaa accaggttcg caagccgacc ctggaccttc cgctcgaagt
    41401 gcggcgcaaa atgtggttca aaccgttcat gcaatcctac ctggtggtct ttatcggcta
    41461 cctgacgatg tacctgattc gcaagaactt taacatcgcg cagaacgaca tgatctcgac
    41521 ctacgggttg agcatgacgc agctggggat gatcggcctg ggtttctcca tcacttatgg
    41581 cgtgggtaaa acgctggttt cctactacgc cgacggcaaa aacaccaaac aattcctgcc
    41641 gttcatgctg atcctgtctg ccatttgtat gctgggcttc agtgccagta tgggcagcgg
    41701 ctcggttagc ctgttcctga tgattgcctt ctacgcctta agcggctttt tccagagtac
    41761 cggcggttcg tgcagttact ccaccatcac caaatggacg ccgcgtcgta aacgcgggac
    41821 attcctcggt ttctggaata tttctcacaa ccttggcggt gcaggcgcag caggtgtggc
    41881 gctgttcggg gcaaattacc tgttcgatgg ccatgtcatc ggcatgttta tcttcccgtc
    41941 gattatcgcg ctgattgtcg gttttatcgg cctgcgttac ggcagcgact ccccggaatc
    42001 ttatggcctc ggcaaagctg aagaactgtt cggcgaggag atcagcgaag aggacaaaga
    42061 gacagaatct accgatatga ccaagtggca gatctttgtt gagtatgtgc tgaaaaacaa
    42121 agtgatctgg ctgctgtgct tcgccaacat tttcctctat gtggtacgta ttggtatcga
    42181 ccagtggtca accgtatacg cgttccagga actgaaactc tctaaagcgg tggcgattca
    42241 gggctttacg ctgtttgaag ctggtgcgct ggtcggtacg ctgctgtggg gctggctctc
    42301 tgacctggcg aacggtcgcc gtggcctggt ggcctgcatc gcgctggcgc tgattatcgc
    42361 cacgctcggt gtgtatcaac atgccagcaa cgaatatatc tatctggctt ctctctttgc
    42421 gttgggtttc ctggtctttg gcccgcaatt gttgattggt gtggctgctg ttggctttgt
    42481 acctaaaaaa gcgattggcg ctgccgatgg tattaaaggc acctttgctt acctgattgg
    42541 tgacagcttt gccaagttag gtctgggaat gattgccgat gggacgccgg tattcggcct
    42601 taccggctgg gcaggcacct tcgccgcact tgatatcgcc gcgattggtt gtatctgcct
    42661 gatggcgata gtggcggtga tggaagaacg caaaatccgc cgcgagaaaa aaattcagca
    42721 gttgacagtg gcataaacgt aactggtgac ttttgcccgg catgacgccg ggctttttta
    42781 ttattccgtg acttccagcg tagtgaaggc aaacttctcg ccatcaaata gcccctgact
    42841 ggttagtttt agcgcgggga tcactggcag agaaagaaac gccatctgaa taaacggctc
    42901 atcgggtaac ggaccgcatt cacgggcggc ggctttcaag gcgtcaattt gttccgccag
    42961 cgactgcgcc gtgtcggtgc tcatcaggcc ggcaatgggc aacggcagat gactttggac
    43021 ctggccgtta cgtaccacgc acagcccgcc gccatcctga atcacctgat tgaccgccag
    43081 cgccatctct tcggcactgc gaccgatcac cacaatatta tggctgtcat ggctgaccgt
    43141 cgcagccagc gcaccttcat tcagtccaaa gccgccaagc aaaccacaag ccggagccag
    43201 ccgttgcccg taacgctcaa gtacggcaat aaagctcaca tcatcgcgat caaaaccatt
    43261 ttcgctgtag acgctggagt gggagtgcgt aatcaattcg ttatggatga cgtcaatgac
    43321 ccgatagcgt tttccgggcg taaattgcag ggcaaagtcg ctggcggaaa ctggctggcg
    43381 ggcaatggtg ttgccatatg gcggagcgga ttgtgccagt ctcgccgact cttccgcctg
    43441 taaggtttgc gcatcaatcg gctcgccttt caccagtacc tgctgcaccg tgaccttacg
    43501 cgcatcgctc aacaggacga tatcggcctg cttgccgggt gccagtaagc cgaggtgatt
    43561 cagaccaaag tggcgcgccg tcgaccagct ggcgacgcga tatgccacat gcagcggcac
    43621 attgtgttgt tcgatcaggc ggcgaattaa ggcatcgatg tgtccttcat gggcgatctc
    43681 ccacgggtta cggtcatcgg tacagagcat gcattgcggg ctgttaaatt cgttgatcaa
    43741 cggtgccagc gcgttgagat tgcgggcagc ggacccttcg cggatcatca acgacatgcc
    43801 gagttgtaat ttccggcgtc cttcttccag ctgataactt tcgtggcagt tttcaatacc
    43861 cgcagtaata taggcgttaa gttctttacc acccaaaccc gggcagtgac cgtccagcgt
    43921 caggtggcga aatgcatcca gtttatcgag cagcgcattc tgcccgctaa ttacgccagg
    43981 gtagtccatc atttctgcaa ggccggtaac ctgcggatgg tcccgccagg cgagcatctg
    44041 ttcaagggta aaactggcac cgttaacatc gcagccttcc agggcgggta cgcaagagct
    44101 gacctgtaag tactggtttt gccttgcctg ttcggcacag cgggcaaacc aggcgaatcc
    44161 ggcttctccc atcacgttga cgatttcatg agggtcgcaa ataacggtcg tcaggccgcg
    44221 cggcagggta gcggtttcaa aagtgaccgg cgtcatcatg ctggattcaa tatgcaggtg
    44281 agcatcaata aaccctggca ccgccgttgc gccgcgagca tcaatccgct gcaaagccgg
    44341 agcatcagcg tattctgcgc caacaccggc aatgtaacgt cctttaatca caattgggcc
    44401 ggaaatttct ccgccattga tcaggtcgag aatagagaca ttatcaataa tataatcggc
    44461 aacagcgtcg ccacgggaaa cggctaacaa ttcctggtat tcagcccggc taatgtgatg
    44521 aaattt
//
LOCUS       DAPGWD010000023        44148 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPGWD010000023 DAPGWD010000000
VERSION     DAPGWD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.023"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            53..1006
                     /locus_tag="RHX88_001195"
     CDS             53..1006
                     /locus_tag="RHX88_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2229917.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1047..2042)
                     /gene="pgl"
                     /locus_tag="RHX88_001196"
     CDS             complement(1047..2042)
                     /gene="pgl"
                     /locus_tag="RHX88_001196"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDV2229918.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2197..3015
                     /gene="ybhA"
                     /locus_tag="RHX88_001197"
     CDS             2197..3015
                     /gene="ybhA"
                     /locus_tag="RHX88_001197"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDV2229919.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3016..4074)
                     /gene="modC"
                     /locus_tag="RHX88_001198"
     CDS             complement(3016..4074)
                     /gene="modC"
                     /locus_tag="RHX88_001198"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDV2229920.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4077..4766)
                     /gene="modB"
                     /locus_tag="RHX88_001199"
     CDS             complement(4077..4766)
                     /gene="modB"
                     /locus_tag="RHX88_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDV2229921.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4766..5539)
                     /gene="modA"
                     /locus_tag="RHX88_001200"
     CDS             complement(4766..5539)
                     /gene="modA"
                     /locus_tag="RHX88_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2229922.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5706..5855)
                     /gene="acrZ"
                     /locus_tag="RHX88_001201"
     CDS             complement(5706..5855)
                     /gene="acrZ"
                     /locus_tag="RHX88_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDV2229923.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5984..6772
                     /gene="modE"
                     /locus_tag="RHX88_001202"
     CDS             5984..6772
                     /gene="modE"
                     /locus_tag="RHX88_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDV2229924.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6840..8312
                     /gene="modF"
                     /locus_tag="RHX88_001203"
     CDS             6840..8312
                     /gene="modF"
                     /locus_tag="RHX88_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDV2229925.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8573..9589
                     /gene="galE"
                     /locus_tag="RHX88_001204"
     CDS             8573..9589
                     /gene="galE"
                     /locus_tag="RHX88_001204"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDV2229926.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9599..10645
                     /gene="galT"
                     /locus_tag="RHX88_001205"
     CDS             9599..10645
                     /gene="galT"
                     /locus_tag="RHX88_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDV2229927.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10649..11797
                     /gene="galK"
                     /locus_tag="RHX88_001206"
     CDS             10649..11797
                     /gene="galK"
                     /locus_tag="RHX88_001206"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDV2229928.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11791..12831
                     /gene="galM"
                     /locus_tag="RHX88_001207"
     CDS             11791..12831
                     /gene="galM"
                     /locus_tag="RHX88_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDV2229929.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            13036..13788
                     /gene="gpmA"
                     /locus_tag="RHX88_001208"
     CDS             13036..13788
                     /gene="gpmA"
                     /locus_tag="RHX88_001208"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDV2229930.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VLPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13946..14998)
                     /gene="aroG"
                     /locus_tag="RHX88_001209"
     CDS             complement(13946..14998)
                     /gene="aroG"
                     /locus_tag="RHX88_001209"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDV2229931.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15314..15694
                     /locus_tag="RHX88_001210"
     CDS             15314..15694
                     /locus_tag="RHX88_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDV2229932.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15808..16749
                     /gene="zitB"
                     /locus_tag="RHX88_001211"
     CDS             15808..16749
                     /gene="zitB"
                     /locus_tag="RHX88_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDV2229933.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16746..17465)
                     /gene="pnuC"
                     /locus_tag="RHX88_001212"
     CDS             complement(16746..17465)
                     /gene="pnuC"
                     /locus_tag="RHX88_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDV2229934.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17503..18546)
                     /gene="nadA"
                     /locus_tag="RHX88_001213"
     CDS             complement(17503..18546)
                     /gene="nadA"
                     /locus_tag="RHX88_001213"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDV2229935.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18795..18920)
                     /locus_tag="RHX88_001214"
     ncRNA           complement(18795..18920)
                     /ncRNA_class="other"
                     /locus_tag="RHX88_001214"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(18951..19026)
                     /locus_tag="RHX88_001215"
     tRNA            complement(18951..19026)
                     /locus_tag="RHX88_001215"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18991..18993),aa:Lys,seq:ttt)
     gene            complement(19031..19106)
                     /locus_tag="RHX88_001216"
     tRNA            complement(19031..19106)
                     /locus_tag="RHX88_001216"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19071..19073),aa:Val,seq:tac)
     gene            complement(19151..19226)
                     /locus_tag="RHX88_001217"
     tRNA            complement(19151..19226)
                     /locus_tag="RHX88_001217"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19191..19193),aa:Val,seq:tac)
     gene            19485..20900
                     /gene="gltX"
                     /locus_tag="RHX88_001218"
     CDS             19485..20900
                     /gene="gltX"
                     /locus_tag="RHX88_001218"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDV2229936.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(20952..21344)
                     /locus_tag="RHX88_001219"
     CDS             complement(20952..21344)
                     /locus_tag="RHX88_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV2229937.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(21346..21705)
                     /locus_tag="RHX88_001220"
     CDS             complement(21346..21705)
                     /locus_tag="RHX88_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV2229938.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            21926..22001
                     /locus_tag="RHX88_001221"
     tRNA            21926..22001
                     /locus_tag="RHX88_001221"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21959..21961,aa:Ala,seq:ggc)
     gene            22041..22116
                     /locus_tag="RHX88_001222"
     tRNA            22041..22116
                     /locus_tag="RHX88_001222"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22074..22076,aa:Ala,seq:ggc)
     gene            22324..24513
                     /locus_tag="RHX88_001223"
     CDS             22324..24513
                     /locus_tag="RHX88_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDV2229939.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(24563..25765)
                     /gene="nupC"
                     /locus_tag="RHX88_001224"
     CDS             complement(24563..25765)
                     /gene="nupC"
                     /locus_tag="RHX88_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDV2229940.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            26101..27339
                     /locus_tag="RHX88_001225"
     CDS             26101..27339
                     /locus_tag="RHX88_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDV2229941.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(27480..27806)
                     /locus_tag="RHX88_001226"
     CDS             complement(27480..27806)
                     /locus_tag="RHX88_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV2229942.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(27921..29177)
                     /locus_tag="RHX88_001227"
     CDS             complement(27921..29177)
                     /locus_tag="RHX88_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDV2229943.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIALALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            29381..30346
                     /gene="glk"
                     /locus_tag="RHX88_001228"
     CDS             29381..30346
                     /gene="glk"
                     /locus_tag="RHX88_001228"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDV2229944.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            30565..30891
                     /locus_tag="RHX88_001229"
     CDS             30565..30891
                     /locus_tag="RHX88_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDV2229945.1"
                     /translation="MSKKLIALCACPMGLVHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            30913..32160
                     /locus_tag="RHX88_001230"
     CDS             30913..32160
                     /locus_tag="RHX88_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDV2229946.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            32175..33260
                     /gene="ypdF"
                     /locus_tag="RHX88_001231"
     CDS             32175..33260
                     /gene="ypdF"
                     /locus_tag="RHX88_001231"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV2229947.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            33260..34297
                     /gene="ypdE"
                     /locus_tag="RHX88_001232"
     CDS             33260..34297
                     /gene="ypdE"
                     /locus_tag="RHX88_001232"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV2229948.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRTVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            34322..36817
                     /gene="ptsP"
                     /locus_tag="RHX88_001233"
     CDS             34322..36817
                     /gene="ptsP"
                     /locus_tag="RHX88_001233"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV2229949.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELACQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQYFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(36820..37677)
                     /locus_tag="RHX88_001234"
     CDS             complement(36820..37677)
                     /locus_tag="RHX88_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2229950.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(37690..38424)
                     /gene="ypdB"
                     /locus_tag="RHX88_001235"
     CDS             complement(37690..38424)
                     /gene="ypdB"
                     /locus_tag="RHX88_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDV2229951.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(38439..40136)
                     /gene="ypdA"
                     /locus_tag="RHX88_001236"
     CDS             complement(38439..40136)
                     /gene="ypdA"
                     /locus_tag="RHX88_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDV2229952.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIICDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            40513..41751
                     /gene="alaC"
                     /locus_tag="RHX88_001237"
     CDS             40513..41751
                     /gene="alaC"
                     /locus_tag="RHX88_001237"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDV2229953.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(41816..41887)
                     /gene="ypdK"
                     /locus_tag="RHX88_001238"
     CDS             complement(41816..41887)
                     /gene="ypdK"
                     /locus_tag="RHX88_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDV2229954.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(42243..43163)
                     /gene="lpxP"
                     /locus_tag="RHX88_001239"
     CDS             complement(42243..43163)
                     /gene="lpxP"
                     /locus_tag="RHX88_001239"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDV2229955.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMLGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            43516..43758
                     /locus_tag="RHX88_001240"
     CDS             43516..43758
                     /locus_tag="RHX88_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDV2229956.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(43835..44110)
                     /gene="ypdI"
                     /locus_tag="RHX88_001241"
     CDS             complement(43835..44110)
                     /gene="ypdI"
                     /locus_tag="RHX88_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HDV2229957.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGGSERSYKETEKTSAM
                     TITHSTKLQPSQAILFKMREDAPPLNLTEETTPPFPTKANYLIHPVR"
BASE COUNT        10800 a        11146 c        11416 g        10786 t
ORIGIN      
        1 atttttaacg accagccacc caaaatcgtc gcttccattt tccggagtaa taatgaagca
       61 tgaattatca agtatgaagg catttgtcat actggcagag tccagttcat ttaacaatgc
      121 cgctaaatta ctcaatatta cgcaacctgc attaacgcga agaataaaaa agatggagga
      181 agatttacat attcaactat ttgagcgtac aactcgcaag gttacgttaa caaaagcagg
      241 aaaaaggttg ctcccggagg cgcgggaatt aataaagaaa ttcgatgaaa cgctttttaa
      301 tattcgtgat atgaatgctt atcatcgtgg tatggtgacg ttagcgtgta ttccgaccgc
      361 agtattttat tttttaccgc tggcaatcgg taaatttaat gagctgtatc ccaatattaa
      421 agtgcggatt ctggaacaag gcacgaataa ttgcatggaa tcggtgctgt gtaacgaatc
      481 agactttggc ataaacatga acaacgtcac aaattcatcc attgatttta ccccactggt
      541 caacgagcca ttcgtgctgg cctgtcggcg tgaccatcca ttggccaaaa agcagcttgt
      601 agaatggcag gagttagtgg gttacaaaat gattggcgta cgttcctcca gcggcaaccg
      661 attgttgata gaacaacagc tagccgacaa gccctggaag ctggactggt tttacgaagt
      721 gcgtcatctt tcgacgtcat taggactagt ggaggcagga ctggggattt cggcgcttcc
      781 tggtctggca atgccccatg cgccctattc ttcgattatt ggtatcccac tggtggaacc
      841 cgttatacgt cggacattgg ggattattcg ccgcaaagat gccgtgcttt ctccggcagc
      901 tgaacgcttt tttgccttgc tgattaattt gtggactgac gataaagaca atttgtggac
      961 caatatcgtc gaacgccaga gacatgcgtt acaggagata ggttaataca ccggcgcagg
     1021 agagactgcg ccggttaaat cagcggttag tgtgcgttaa ccaccaccca cattggtccc
     1081 tgcccgaccg cataacggcc tttttcatgc agtagtccct gctcgccaac aatttcgtat
     1141 accgagatgt ggtgagattt ttgcccggcg gcaatcagat acttgccgct gtgatcaaca
     1201 ttgaagccgc gcggctgggt ttccgttggc tggaagcctt ctttactcaa cacgctgcca
     1261 tcttccgaaa cgctgaaaac ggtaatcagg ctggcggtac ggtcgcaggc gtataaatgg
     1321 cgaccatccg gggtgatatg aatatcagcc gcccaacggg tgtcggagaa gttttccggc
     1381 atcatatcca gcgtctggac acattcgata ttaccgtgcg gatctttcag ttcccagaca
     1441 tccactgagc tgtttaactc attgacgcaa tacgcatatt gttcgtttgg atggaatacc
     1501 atatgacgcg ggccggcccc ttcaacggtg gtcacttccg cagggtcctg cgccacgaga
     1561 tgaccatcat cgctgaccgt aaacaggcaa atgcgatcct gctttaatgc cggaacccac
     1621 agcgtacggt tgtccggtga gatattggcg gaatggcaac cgtccagccc ctcgaccaca
     1681 tcgacgacgc ccactggcag gccatcttcc agacgcgtta cgctcacgtt acccgcattg
     1741 taagaaccta caaagacaaa ctgcccctgg tgatcggtgg aaatatgcgt cggactaccc
     1801 ggcagcgcag actctgcggc aaaggtcagt gcgccatcgt ccggggcgat acgatacgcc
     1861 aggacgcgaa actcagggcg aacaccaaca tagagataac gtttgtccgg gctgaccacc
     1921 atcggctgca cctgccccgg cacatcgaca acctgtgtca gcgtcagtgc gccttcatga
     1981 ttcaggttcc agacgtgaat ttgctggctc tcagggctgg cgatataaac tgtttgcttc
     2041 atgaatgctc ctttgcattt agcaactgac tgtaacagct aaaattagtc gcttttggcg
     2101 gtgaatgctg aattaaaacg cagaattttg tccgatagtg gtatcggtgt accattcgcg
     2161 cagacgaaaa ttcttaacct taatctggaa tacgccatga ccacacgcgt gattgctctc
     2221 gacttagacg gcaccttatt gaccccgaaa aagaccctgc ttccttcatc gatagaagcc
     2281 ctggcccgcg ctcgcgaagc aggctatcaa ttaatcatcg tcacaggtcg ccatcacgtc
     2341 gctattcatc ctttttatca ggcgctggcg ctggatacac ctgctatttg ctgtaatggc
     2401 acctatttgt atgattatca tgcaaaaacc gtgctggaag cggacccaat gcccgttaat
     2461 aaagccctgc aactcattga gatgctgaat gaacaccaca ttcacggtct gatgtatgtg
     2521 gatgatgcaa tggtctatga gcacccgacc gggcatgtca ttcgcacgtc taactgggcg
     2581 caaaccctgc cgccggaaca gcgtccgact ttcacacaag tcgcttctct ggctgaaacg
     2641 gcgcaacaag ttaacgccgt atggaagttc gccctgacgc acgatgacct gccgcaattg
     2701 cagcattttg gtaagcatgt cgaacatgaa ctgggactgg agtgtgaatg gtcctggcac
     2761 gatcaggttg atattgcacg cggcggcaac agcaaaggta aacgtttgac gaaatgggtt
     2821 gaggcgcaag gttggtcgat ggaaaacgtc gtggcattcg gcgataactt taatgatatc
     2881 agtatgctgg aagccgctgg tacaggcgtg gcgatgggca acgccgatga cgcggtaaaa
     2941 gcgcgcgcca acattgtgat tggtgataac accaccgaca gcattgccca gttcatttat
     3001 agccacctga tttaatcagg cggttatcga cacactttta atttgcgcgt acagccacag
     3061 gcctggtttg atcgccagtt catccctggc ccacgggctg atacgcgccc acagcgtttt
     3121 accgccgact tccagctcca cttccacctg accgttgtcg tcataactat taacaacttt
     3181 tgcccgcaat acgttacgaa tgctggtttg ctgcggcggt tgtaaaacca gagaaacatc
     3241 ggaagcctga atgcggatac gtagcgcagc ttgcagcggt tcgtccagct tattgaccca
     3301 caaatgctga tcgcccagcg ccagcgcggt catcgcgtaa tgcggatgat gctcaagcac
     3361 cgtcactttc agaatgctac tttgttgctc tttcggcagc cacggattca tcacgctact
     3421 accccagact tcttccagtg cgccaaaggc tttcacctga ccgttttcca gtaccatcac
     3481 tctgtctgcc aaatggagga tctcatccag cgaatggctg acatacaaca tcgggatgtt
     3541 gatttcccgc gtcagacgtt gcagataagg caacagttcg cgtttacgcg gaatatccag
     3601 tgacgccagc ggttcatcca gcaacagcaa ttctggtgcc gtcagcaaag cccgcccaat
     3661 cgctacgcgc tgtttttcgc ctccggacag gctgcctggt aaacggtcaa gcaacggttc
     3721 aatgcctaaa agcgccacca gcttatcgaa ctgatcgacc atgcttttcg ccatgccgta
     3781 gcgcagattg ccacgcactt tgtaatgcgg aaacagccgc gcatcctgaa aaacatagcc
     3841 aacgcgacgc ttttctggcg tcaggcagat acctttttcg gcatcattta gtacccgacc
     3901 attgaggaca atcctccctt tttgcgggcg cgtcagtcca ctgatggcgt taatcagcga
     3961 agtttttccg gcaccggaga cgccaaagat agcagtgatg ccattggcgg gcagcgtttc
     4021 attaatggtc aggcaatggt tgcccaaggt ctgggaaaaa ttcagttcca gcatgattag
     4081 cgccccgccc gttcacggct gattctggcc agccattctg aaatcaacag ggagatcatc
     4141 gccagcgcaa tagaaataat gcacagtctc gccgcgccac tttccccgcc gggggtctgg
     4201 atcagggtat acatggcaga aggaatggtt cgcgtttcac caggaatgtt cgacacaaag
     4261 gtgatggttg caccaaactc accgagagaa cgagcaaaag ccagtaccgt accaacaata
     4321 attcccggta aggtcagcgg taacgtgata gtaaagaaaa cgcgccagcg cccggccccc
     4381 agtgttcttg cggcctgttc gagtttgaca tcaacccctt ccagcgccag acgaattgcc
     4441 cgcaccatca gcggaaacga catgacggcg gcagcgagaa ccgcgccgcg ccagctaaag
     4501 gcgaaggtaa taccaaacca gtcatacaga cgttcaccga taaatccgcg ccgtcccatc
     4561 gaaactaata ataagtaacc gacgaccacg ggcggtaaca ccagcggtag atgcagtacg
     4621 ctgtcgagca gagctttgcc cggaaacgtg caacgcacca gtaaccaggc aaaaaagatc
     4681 ccaaacggca ggctaaacag cacagccagg gaagaaactt tcaggcttaa taaaactgcc
     4741 tgccattctg gatcggtcag tatcattact tggttgtaaa tccgtaacgt ttaaagattt
     4801 ccgctgcctg cggtcccttc agataatcat aaaatgcttt cactgtcgca ttgttatgcc
     4861 cttccacaac agcaaccgga tattccactt ttttatgtga atcttccggg aaggtggcaa
     4921 ccacttttac ccctttgctg acaactgcgt cagaaccgta aacaatgccc aacggcgctt
     4981 cattgcgttc gaccaacgcc agcgcgccac gaacatcttc cgccggagcc agtttcgggg
     5041 agagcgtatc ccatgcgccc agtttttgca gtgcttcttt tgcataaatg ccagcaggga
     5101 catgttccgg atcgccaacc gccaggcgac cgccattcag cagcgaagtc cagttggttt
     5161 tgctgtcgat gatgaaatct ttctgctcgc tggctttcgg tgctacaacg accaggctat
     5221 tgccgagcag tgtctggcgc gaagccgtat cgatcgcttt tttatcaacc gcataatcca
     5281 tccatttctg atcggcagag ataaacagat ccgcaggcgc tcccgcttca atctgacggg
     5341 ccagagtaga tgacgaagcg aaagaagaaa ccacatccac gcctttttct tttttatact
     5401 gcgtagcaat gtcctgcatt gcgttagtca gtgatgcggc ggcgaacacc gtgattttcc
     5461 cttcatctgc cagtgtattg ccagcaacag cgaaagagag tgccgccccg gcaaacaagt
     5521 tcaaccattt acgagccatt ggtaacccct taatgtaacg ttatgtaggc gacaatataa
     5581 cggctaactc ggaattttcc cagcagttat tgctaacctt tgtgaggtag ataagaaaaa
     5641 atatcggcag gaaaagcagg aagttgagaa aaagaaaatg cccgactaag cgggcattca
     5701 gggaatcaat gattttgtcc gggctggtct tttttaccaa caccagaaaa gatgttgaat
     5761 acttcaccaa gaccgtaaat cagacccagg atgatggcca tcacgacagg taccataatt
     5821 acggcgaata ccagactttt taataactct aacatggtca actccagata tagtcatgag
     5881 actattctaa ccgctaagca cagaaaagca ctcccctttt gtgcggtcag ctttgcgtgg
     5941 cgttcgtttt ccgtcacaat aagacttttg ccaggacatt gttatgcagg ccgaaatcct
     6001 tctcaccctt aagctccaac aaaaattatt cgccgacccg cgccgcattt cgctactaaa
     6061 acacattgcg ctttccggtt ccattagcca gggagcgaaa gatgccggta tcagctataa
     6121 aagcgcctgg gatgccatta acgagatgaa tcagttaagt gagcatattc tggtcgagcg
     6181 cgcaacaggc ggtaaaggtg gcggcggcgc agtactgacc cgctatggtc agcgactgat
     6241 tcagctctat gacttactgg cgcaaatcca gcaaaaagcc tttgatgtgt taagtgacga
     6301 tgacgccctg ccgctgaaca gcctgctggc cgcgatctca cgtttttcac tgcaaaccag
     6361 cgcccgtaac cagtggttcg gcaccatcac cgcccgcgat catgatgacg ttcaacagca
     6421 tgttgatgtc ttactggctg acggaaaaac acgcctgaaa gtcgcaatta ccgcacaaag
     6481 cggcgcacgt ctggggctgg atgaaggcaa agaagtgttg atattgctaa aagcgccgtg
     6541 ggtaggcatt actcaggacg aagcggtcgc acaaaacgcc gacaaccaat taccgggtat
     6601 tattagtcat attgagcgcg gcgcagagca gtgcgaagta ttaatggcgc tacccgacgg
     6661 gcaaacactg tgcgccacag tgccggttaa tgaagcgact tctcttcagc aaggacagaa
     6721 tgtcacggcc tactttaatg ccgacagcgt gattatcgcc acgctatgct aagcgtgttg
     6781 acaatttgtt atgaaacacg tatccctatc agtaatcgct gcacaaaatg ggatataaaa
     6841 tgtcatcgtt gcaaattttg caaggcacgt ttcgtcttag cgacacaaaa acgctgcaat
     6901 tgcctcagct aacgttaaac gcgggtgata gttgggcgtt tgtcggttcg aatggaagtg
     6961 gaaagtcggc gctggcccgt gcgctggcgg gggaacttcc gcttttgaaa ggtgaacggc
     7021 aaagccagtt ttcccacatc actcgtctct ccttcgagca attgcaaaag ctcgtcagcg
     7081 acgaatggca gcggaataac accgatatgc tcggccctgg cgaagatgac accggacgca
     7141 ctacggctga gatcattcag gatgaagtta aggatgcacc gcgttgcatg caattggcgc
     7201 agcagttcgg tattaccgcc ctcctcgacc gacgctttaa atacctttcc actggcgaga
     7261 cgcgaaaaac cctgctgtgt caggcgctga tgtcggagcc tgacttgttg attcttgatg
     7321 agccgttcga tggcctggat gttgcctcac gtcagcagct ggctgagcga ctcgcctcgt
     7381 tacatcagtc cggtattact ctggtactgg tgctcaatcg cttcgatgag atcccggaat
     7441 ttgtccagtt tgctggcgtg ctggcggatt gtacgttagc ggaaacgggc gctaaagagg
     7501 aactgctcca gcaagcactc gtcgcgcaac tggcgcatag cgaacagctt gaaggtgtgc
     7561 aactgccgga gccggatgaa ccttcagcac gtcacgcctt acccaccaac gaaccgcgca
     7621 ttgtgctgaa taatggcgtg gtttcttata acgatcgccc cattcttaat aaccttagct
     7681 ggcaggtgaa tcctggcgag cactggcaaa ttgtcgggcc aaatggtgcg ggaaaatcga
     7741 cgttattaag cctgattact ggcgatcatc cgcaaggtta cagcaacgat ttgacgcttt
     7801 tcggccgacg tcgcggcagc ggcgaaacca tctgggatat caaaaagcat atcggttacg
     7861 tcagcagtag tttgcatctg gattaccggg tcagcactac cgtgcgtaat gtgattcttt
     7921 ctggctattt tgattcgatt ggcatttatc aggccgtttc ggatcgccag caaaaactgg
     7981 tgcagcagtg gctggatatt ctcggcattg ataaacgtac ggctgacgct ccgttccata
     8041 gtctttcctg gggacagcag cgtctggcgc tgatcgtccg cgcactggtg aaacatccga
     8101 cgttgcttat tctcgatgaa ccactacagg ggcttgatcc gctcaatcgc cagcttatcc
     8161 gccgttttgt tgatgtgctg attagcgaag gtgaaacgca attgttgttt gtttcgcacc
     8221 acgctgaaga tgcgcctgcc tgtattaccc atcgccttga gttcgtgccg gacggtggac
     8281 tctatcgcta tgtgctgaca aaaatatatt gagtcggtag tgctgacctt gccggaggcg
     8341 gcctaagcgc cctctccggc caacggttcg acgcatgcag gcatgaaacc gcttcttttt
     8401 tcagataaaa agcgcaatca ttcataaacc ctctgtttta taatcactta atcgcgcata
     8461 aaaaacggct aaattcttgt gtaaacgatt ccactaattt attccatgtc acacttttcg
     8521 catctttgtt atgctatggt tatttcatac cataagccta atggagcgaa ttatgagagt
     8581 tctggttacc ggtggtagcg gttacattgg aagtcatacc tgtgtgcaat tactgcaaaa
     8641 cggtcatgat gtcatcattc ttgataacct ctgtaacagt aagcgcagcg tactgcctgt
     8701 tatcgagcgt ttaggcggca aacatccaac gtttgttgaa ggcgatattc gtaacgaagc
     8761 gttgatgacc gagatactgc acgatcacgc tatcgacacc gtgatccact tcgccgggct
     8821 gaaagccgtc ggcgaatcgg tacaaaaacc gctggaatat tacgacaaca atgtcaacgg
     8881 cactctgcgc ctgattagcg ccatgcgcgc cgctaacgtc aaaaacttta tttttagctc
     8941 ctccgccacc gtttatggcg atcagcccaa aattccatac gttgaaagct tcccgaccgg
     9001 cacaccacaa agcccttacg gcaaaagcaa gctgatggtg gaacagatcc tcaccgatct
     9061 gcaaaaagcc cagccggact ggagcattgc cctgctgcgc tacttcaacc cggttggcgc
     9121 gcatccgtcg ggcgatatgg gcgaagatcc gcaaggcatt ccgaataacc tgatgccata
     9181 catcgcccag gttgctgtag gccgtcgcga ctcgctggcg atttttggta acgattatcc
     9241 gaccgaagat ggtactggcg tacgcgatta catccacgta atggatctgg cggacggtca
     9301 cgtcgtggcg atggaaaaac tggcgaacaa gccaggcgta cacatctaca acctcggcgc
     9361 tggcgtaggc aacagcgtgc tggacgtggt taatgccttc agcaaagcct gcggcaaacc
     9421 ggttaattat cattttgcac cgcgtcgcga gggcgacctt ccggcctact gggcggacgc
     9481 cagcaaagcc gaccgtgaac tgaactggcg cgtaacgcgc acactcgatg aaatggcgca
     9541 ggacacctgg cactggcagt cacgccatcc acagggatat cccgattaag gaacgaccat
     9601 gacgcaattt aatcccgttg atcatccaca tcgccgctac aacccgctca ccgggcaatg
     9661 gattctggtt tcaccgcacc gcgctaagcg cccctggcag ggggcgcagg aaacgccagc
     9721 caaacaggtg ttacctgcgc acgatccaga ttgcttcctc tgcgcaggta atgtgcgggt
     9781 gacaggcgat aaaaaccccg attacaccag gacttacgtt ttcactaatg actttgcggc
     9841 cttgatgtct gacacgccag atgcgccaga aagcaacgat cctttgatgc gttgccagag
     9901 cgcgcgcggt accagccggg tgatctgctt ttcaccggat cacagtaaaa cgttgccaga
     9961 actgagcgtt gcggcattga cggaaatcgt caaaacctgg caggagcaaa ccgcagagct
    10021 gggaaaaacg tacccgtggg tgcaggtctt tgaaaacaaa ggtgcggcga tgggctgctc
    10081 taacccgcat ccgcacggac agatttgggc aaatagcttc ctgcctaacg aagctgagcg
    10141 cgaagaccgc ctgcaaaaag aatattttgc cgaacagaaa tcaccaatgc tggtggatta
    10201 tgttcagcgc gagctggcag acggtagccg taccgttgtc gaaaccgaac actggttagc
    10261 cgttgtgcct tactgggctg cctggccgtt cgaaacgcta ctgctgccca aagcccacgt
    10321 tttgcggatc accgatttga ccgacgccca gcgcagcgat ttggcgctgg cgttgaaaaa
    10381 gctgaccagt cgttatgaca acctcttcca gtgttccttc ccctactcta tgggctggca
    10441 cggcgcacca tttaatggcg aagagaatca acactggcag ctgcacgcgc acttttatcc
    10501 gcctctgttg cgctccgcca ccgtacgtaa atttatggtt ggttatgaaa tgctggcaga
    10561 gacccagcga gacctgaccg cagaacaggc agcagagcgt ttgcgcgcag tcagcgatat
    10621 ccattttcgc gaatccggag tgtaagaaat gagtctgaaa gaaaaaacac aatctctgtt
    10681 tgccaacgca tttggctacc ctgccactca caccattcag gcgcctggcc gcgtgaattt
    10741 gattggtgaa cacaccgact acaacgacgg tttcgttctg ccctgcgcga ttgattatca
    10801 aaccgtgatc agttgtgcac cacgcgatga ccgtaaagtt cgcgtgatgg cagccgatta
    10861 tgaaaatcag ctcgacgagt tttccctcga tgcgcccatt gtcgcacatg aaaactatca
    10921 atgggctaac tacgttcgtg gcgtggtgaa acatctgcaa ctgcgtaaca acagcttcgg
    10981 cggcgtggac atggtgatca gcggcaatgt gccgcagggt gccgggttaa gttcttccgc
    11041 ttcactggaa gtcgcggtcg gaaccgtatt gcagcagctt tatcatctgc cgctggacgg
    11101 cgcacaaatc gcgcttaacg gtcaggaagc agaaaaccag tttgttggct gtaactgcgg
    11161 gatcatggat cagctaattt ccgcactcgg caagaaagat catgccttgc tgattgactg
    11221 ccgctcactg gggactaaag cagtttccat gccgaaaggt gtggctgtcg tcatcatcaa
    11281 cagtaacttc aaacgtaccc tggttggcag cgaatacaac acccgtcgtg aacagtgcga
    11341 aaccggtgcg cgtttcttcc agcagccagc tctgcgcgat gtcaccattg aagagttcaa
    11401 cgctgttgca catgagctgg acccaatcgt ggcgaaacgc gtgcgtcata tcctgactga
    11461 aaacgcccgc accgttgaag ctgccagcgc gctggagcaa ggcgacctga aacgtatggg
    11521 cgagttgatg gcggaatctc atgcctctat gcgcgatgat ttcgaaatca ccgtgccgca
    11581 aattgacact ctggtagaaa tcgtcaaagc tgtgattggc gacaaaggtg gcgtacgcat
    11641 gaccggcggc ggatttggcg gctgtatcgt cgcgctgatc ccggaagagc tggtgcctgc
    11701 cgtacagcaa gctgtcgctg aacaatatga agcaaaaaca ggtattaaag agacttttta
    11761 cgtttgtaaa ccatcacaag gagcaggaca gtgctgaacg aaactcccgc actggcaccc
    11821 gatggtcagc cgtaccgact gttaactttg cgtaacaacg cagggatggt agtcacgctg
    11881 atggactggg gtgcgacttt actttccgcc cgtattccgc tttccgatgg cagcgtccgc
    11941 gaggcgctgc tcggctgtgc cagcccggaa tgctatcagg atcaggctgc gtttctgggg
    12001 gcctctattg gtcgttatgc caaccgcatc gccaatagcc gttatacctt tgacggtgaa
    12061 accgtgacgc tttcgccaag tcagggcgtt aaccagctgc acggcggccc ggaagggttc
    12121 gacaaacgtc gctggcagat tgtgaaccag aacgatcgtc aggtgctgtt tgccctgagt
    12181 tcagatgatg gcgatcaggg cttcccgggt aatctcggcg cgacggtgca atatcgtctg
    12241 accgacgata accgtatctc tattacttat cgcgccacag ttgataaacc ttgcccggtg
    12301 aatatgacta atcacgtcta tttcaatctt gacggcgagc agtctgacgt gcgcaatcac
    12361 aagttgcaga ttctggcgga cgaatatctg ccggttgatg aaggcggcat tccgcacgac
    12421 ggtctgaaat ctgtcgccgg aacgtctttt gatttccgta gcgccaaaat catcgccagt
    12481 gagtttcttg ccgacgacga tcagcgcaaa gtgaaaggtt acgatcacgc attcttgtta
    12541 caggccaaag gcgatggcaa gaaagtggcg gcgcatgtct ggtcagcaga tgaaaaattg
    12601 cagctgaagg tctacaccac cgctccggct ctgcaattct actccggcaa cttcctcggc
    12661 ggcacgccat caagaggaac tgaaccttac gccgactggc aaggcctggc actggaaagc
    12721 gagtttctgc cggacagccc aaaccaccct gaatggccgc aaccggactg cttcctgcgt
    12781 cctggcgaag agtattccag cctgacggaa tatcagttta ttgctgagta gcacctgaag
    12841 ccctcgtaat gagggcttta tccttatatt gctcaaatca ccagcaaaca ccgacatatt
    12901 tgcaactcaa tattcacaac aaccttacac tgcgacacta ttttcgctat ggttatgcgt
    12961 aagcattgct gttgcttcgt cacggcaata taatgagaat tattatcatt aaaaaatgat
    13021 ttgaggagta agtatatggc tgtaactaag ctggttctgg ttcgtcatgg cgaaagtcag
    13081 tggaacaaag aaaaccgttt caccggttgg tacgacgtgg atctgtctga gaaaggcgta
    13141 agcgaagcaa aagcagcagg taagctgctg aaagaggaag gttacagctt tgactttgct
    13201 tacacctctg tgctgaaacg cgctatccat accctgtgga atgtgctgga cgaactggat
    13261 caggcatggc tgcccgttga gaaatcatgg aaactgaacg aacgtcacta cggtgcgttg
    13321 cagggtctga acaaagcgga aactgctgaa aagtatggcg acgagcaggt gaaacagtgg
    13381 cgtcgtggtt ttgcagtgac tccgccggaa ctgactaaag atgatgagcg ttatccgggt
    13441 cacgatccgc gttacgcgaa actgagcgaa aaagaactgc cgctgacgga aagcctggcg
    13501 ctgaccattg accgcgtgct cccttactgg aatgaaacca ttctgccgcg tatgaagagc
    13561 ggtgagcgcg tgatcatcgc tgcacacggt aactctttac gtgcgctggt gaaatatctt
    13621 gataacatga gcgaagaaga gattcttgag cttaatatcc cgactggcgt gccgctggtg
    13681 tatgagttcg acgagaattt caaaccgctg aaacgctatt atctgggtaa tgctgacgag
    13741 atcgcagcga aagcagcggc ggttgcaaac cagggtaaag cgaagtaaac gtcattcgtt
    13801 taaaatgaga aagccgactg caagtgagtc ggcttttttg ttgctaacaa tggagcacat
    13861 tgcctgatgc gacgctgcgc gacttatcag gcctgtggtg attcatcgga tacgccactc
    13921 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcgccagtt
    13981 gacgtaacag agcatcggta tcttcccagc cgatgcaggc atcggtgatg ctcttaccgt
    14041 aggccagcgg ctccccgctc tcgaggctct gattgccttc caccagatgg ctttccacca
    14101 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    14161 ccatctgctt tttgaattgt ttggacgagt tagcatggct gaaatcgatc atcacctgtg
    14221 ctggcaggcc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    14281 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccgctg gtattcacaa
    14341 tcgccgaatg cccccatttc gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    14401 tggcatcgat agccacttta atcgtaccgt cggtgccatt tttgaagccg accggacaag
    14461 aaagccctga tgccagttcg cggtgcacct gcgattcggt ggtacgtgcg ccaattgcgc
    14521 cccagctcat caggtcagcg agatattgtg gggtgatcat atcgagaaac tcacctgccg
    14581 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    14641 tgatctggaa gctattatcc atatgcggat cgttaatcag ccctttccag cccaccgtgg
    14701 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    14761 gcagcgccag caagcgagtg gcatactctt ttgccgcgac aggatcatga attgagcatg
    14821 ggccaatcac aaccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    14881 gggcatgggc aaccgtattc gcggcatttt cagtagcggg gaatttttcc agcaatgcga
    14941 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    15001 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    15061 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    15121 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    15181 tttgttaatt atttcaagat tctctgctgc gtttcataac ctggctgaaa tcttaaacca
    15241 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    15301 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    15361 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    15421 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    15481 taacgtcgac aataacgggg taaataccgg ttctggtggc acaatgctgc attcggatgg
    15541 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    15601 agatggtcgc tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    15661 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    15721 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    15781 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    15841 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    15901 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    15961 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    16021 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    16081 tttgtgaatg ccatagccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    16141 cgcttccgca cgccgcgtcc ggtcgagggc ggcatgatga tggcaattgc cgtggctggg
    16201 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    16261 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    16321 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    16381 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    16441 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    16501 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    16561 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    16621 atccaacact acctgatgga tcactatcag attgagcacg ccaccattca gatggaatat
    16681 caaccttgtc atgggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    16741 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    16801 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    16861 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    16921 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    16981 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    17041 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    17101 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    17161 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccaaccgtaa
    17221 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    17281 aaaataatgc caaacaaggt gacgttgatc aggccaaaga agtagttgct gatcttctcc
    17341 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    17401 agatcataac cgcctgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    17461 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    17521 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tttcatcaac
    17581 atgaacctcg tgattgcttc cttcctgttc taatgcctct gcgatggact gaaggccatt
    17641 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcaccctctc ctgcggttgg
    17701 tgcttccagt aactctttat ctggcactgc ctgctgcatt ttgtagaaaa tgccccgatc
    17761 ggttgccacg ataagccgct gatgtggcaa tgttttcgca gcagcgatca gttgactggt
    17821 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    17881 ggcagcatcc gggtattctt cttgcaagcg agttaacgcc tgagtcttaa attcatcatg
    17941 cacaatacag gcaccctgcc agcatagaat gtctccaccc gtctgttttt gcacgtaacg
    18001 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    18061 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    18121 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgcaaaatg cgttaaattc
    18181 ttcaacaggg cagccgagat ccagtgaaca ttcagcctga agtgtcggca tcagaattgt
    18241 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    18301 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    18361 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    18421 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    18481 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    18541 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaatattc
    18601 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    18661 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    18721 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    18781 gcgctttttt acattggtgg gtcgtgcagg atgactcgct tcgctcgccc ttcgggccgt
    18841 cgccgcgagc ggctccgttg tttcgctgcg ctcaactcaa acctgctgca ggttcgaatc
    18901 tttgagtatc gcagatagca aaaaagcgcc tttagggcgc ttttttacat tggtgggtcg
    18961 tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa
    19021 cgacccgaag tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg
    19081 tgctctccca gctgagctaa tcacccactt ctcaatttct tgctacacgg cggagactac
    19141 ataaagtagt tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg
    19201 tgctctccca gctgagctaa tcacccccgc tgtgtggagt cgcattatag ggagagttca
    19261 aaatgagtca acgcattttc taaagaaatt gttcgttcgt cgtaaattta agcaagatga
    19321 tcgcaaaaca gaccgtgttg cgcaatttgt caacgaaaac aataatgcgt aaggtagaaa
    19381 cccgaactac attgaggaat caggcgggag tgatagaata tcgcccactc aatttttcca
    19441 ggatttgccg gttgtcggca tctttctaaa cgtaaggcca tttcatgaaa atcaaaactc
    19501 gcttcgcgcc aagcccaaca ggctatctgc acgttggcgg cgcgcgtact gctctttact
    19561 cctggctttt tgcacgtaac cacggcggtg agttcgtgct gcgtattgaa gacaccgatc
    19621 ttgagcgttc cacgccggaa gctatcgaag ccattatgga tggcatgaac tggctgagcc
    19681 tggagtggga tgaaggtccg tactaccaga ccaaacgttt tgatcgctac aatgcggtga
    19741 tcgatcagat gctggaagag ggcactgctt ataaatgcta ttgctctaaa gagcgcctgg
    19801 aagcgctgcg cgaagagcaa atggcgaaag gtgagaagcc gcgttatgac ggtcgctgcc
    19861 gccacagcca tgagcatcat gctgatgatg aaccgtgtgt cgtgcgtttt gctaatccgc
    19921 aggaaggttc tgttgttttt gacgatcaga tccgtggtcc gatcgagttc agcaaccagg
    19981 agctggatga tctgattatc cgccgtaccg atggttcccc aacctataac ttctgtgtgg
    20041 ttgtcgatga ctgggatatg gaaatcaccc acgttatccg tggcgaagac catatcaaca
    20101 acacgccgcg ccagatcaac attcttaagg ccctgaaagc gccggtgccg gtttacgcgc
    20161 acgtttctat gatcaatggc gatgacggta aaaaactgtc caaacgtcac ggggcagtca
    20221 gcgtaatgca gtatcgtgat gacggttatt tgccagaagc actgctgaac tatctggtgc
    20281 gtctgggctg gtcccacggc gatcaggaaa tcttcactcg tgaagagatg atcaaatact
    20341 tcactttgaa tgccgtcagc aaatctgcca gtgcgttcaa caccgacaag ctgctgtggc
    20401 tgaaccatca ctacattaac gcgctgccgc cggagtatgt tgctactcac ttacagtggc
    20461 acattgagca ggaaaatatc gatacccgta acggcccgca gctggctgat ctggtgaaac
    20521 tgctgggcga acgctgcaag acgctgaaag agatggcaca gagctgccgt tatttctacg
    20581 aagattttgc tgagttcgat gccgatgcgg cgaaaaaaca tctgcgtccg gtagcgcgtc
    20641 agccgctgga agtggttcgt gacaaactgg ccgcgattac tgactggacc gctgaaaacg
    20701 ttcatcacgc tattcaggcg acggcggatg agctggaagt gggtatgggt aaagttggta
    20761 tgccgctgcg tgtcgccgta accggtgcgg ggcagtctcc agcactggat gttaccgttc
    20821 acgcaattgg taagacccgc agtatcgagc gtatcaacaa agcgctggat tttattgctg
    20881 aacgcgaaaa tcagcagtaa ttagcgtgta aaaagataaa cggcaggaga taatatcctg
    20941 ccgtttttta tttatgctga ttcgtcaata tcgagacggg cgaggaaatt gcgaattcct
    21001 tcacgtgaca aaaattgcgc gactttttcc tgctcaatgt ctgtcatatt atctatcgcg
    21061 ttgatgattt gtcgatacgc cggggcgcgt gctccaggag cgtattcagc aattctctct
    21121 tcagcttcca tcaacattgg tgtgttatgg aaggctggcg tgttctgaat aaactcgcaa
    21181 acttgcgtat cgaccagaat aagtctggca cgaccgcctt tcacgccggg aaatttttcc
    21241 gttttccagc ctttttctct gatccaacgg ttaacagttt gtttagccac accgaggcat
    21301 tcagccagtt cttcggtggt cattttgttg cgtaatcttt tcatattatt tgctatcgcg
    21361 aatccctaag cgttgtaaca atccgataat cccttcccgc agaagcaagg atgtaaactg
    21421 tttttgttca actggcgtca tctctttcgc cagtgtcacc agcaatactt caagcggcgc
    21481 atcaccagaa agggcaggcg cttctccctg accttctgga cgttcagcat tgcgaatata
    21541 ttcacgaacc tgttcattga cgtgaaccag tcgggctttg ccaccctgga cgccaggttt
    21601 tggtgacgtt gtccagcctt ccttgcgtac ccatttatta atggtctggc ggctatagcc
    21661 ggtcagtctg gcaagttcat ctggtgtcat cctctccttg aacataacaa ttccctgaat
    21721 gtatgtgggt ctattagtag ttcattttat aacaccactt tacaggaaac cgtgacatga
    21781 ataactactg aatgcgagcg gttttccaaa attgttcatt tgcctgattt ttcagcgatt
    21841 gaaactacac gttgcaattt gccgttgaca cattcgggcg gaattcatat gatgccgccc
    21901 gtcaactcga caagctttac gtgacggggc tatagctcag ctgggagagc gcttgcatgg
    21961 catgcaagag gtcagcggtt cgatcccgct tagctccacc aaattttgca cccagcaaat
    22021 ttggtacgta aacgcatcgt ggggctatag ctcagctggg agagcgcttg catggcatgc
    22081 aagaggtcag cggttcgatc ccgcttagct ccaccaaatt tccaaccctc gctgcaaagc
    22141 gggggttttt tgtctctgct ttttgccgct tttgtaatac agtctacgtc cgggttagcg
    22201 ccgcctggtg aaagcatcat tggatgaaaa atcggcaaca ggctggcccc tgtttgcttc
    22261 gcgatgcgaa taaacttatt atttgtgtgc ctgaaaaccc cgatcagtga gagtagtgta
    22321 ctcatgtttg tggagcataa cctgataaaa aatatcaaga tattcacact agcgtttacg
    22381 ctcaccgtgg tacttattca gctatcccgt tttatttcgc cacttgccat tatccattcc
    22441 agttatatct ttctggcgtg gatgccactg tgcgtaatgc tgtcaatctt gtttatcttt
    22501 ggctggcgcg gtgtcgttcc cgttttatgc gggatgtttt gcaccaatct gtggaacttt
    22561 catctctctt ttttacagac tgcggtcatg ctcggtagcc agacgtttgt cgtgttgtgt
    22621 gcctgcgcaa tattacgctg gcagctgggg acgcgttggc gttatggatt gaccagccga
    22681 tatgtctggc aacgtctgtt ctggcttggt ttggtgacgc cgatcggcat caaatgcagc
    22741 atgtatcttg tggggagctt ctttgatttc ccgctaaaga tatccacctt tttcggcgat
    22801 gcggatgcca ttttcacggt cgttgatttg ctaagccttt tcaccgctgt gctgatttac
    22861 aacatgcttt tctactatct cactcgcatg attgtaagtc cccactttgc gcagatattg
    22921 tggcgcaggg atatcgctcc gtcgttgggc aaagagaaac gcgcatttac cttaagctgg
    22981 ctggcagcgt taagcgtgct gctacttctg ttgtgcacac cttatgaaaa cgactttatt
    23041 gccggttact tggtacccgt tttcttcatc atctttaccc tcggggtcgg taagcttcgc
    23101 tatccgtttt taaatctcac ctgggctgtt tcaacgcttt gcctgctgaa ttacaaccag
    23161 aactttttgc aaggggtgga aaccgaatat tcgctggcat ttattcttgc ggtgctgatt
    23221 tcctttagcg tttgcctgct ctatatggtg cgcatttatc atcgcagtga atggcttaat
    23281 cgccgctggc atttgcaggc gctgacagat ccgttaacgc tcctacccaa ctttcgtgcg
    23341 ttggaacaag cgccggagca agaggcgggc aagagttttt gctgcctgcg cattgataat
    23401 cttgagttta tgagtcgtca ttacggctta atgatgcgcg ttcactgtat ccgctcaatt
    23461 tgccgtacgc tgctgccgtt gatgcaggaa aacgaaaagt tgtatcaatt gccgggtagt
    23521 gaactgctgt tagtgctgag cgggccggaa acggaagggc gactccagca tatggttaac
    23581 atcctgaata gtcggcaaat tcactggaac aataccgggc tggatatggg ctatggtgct
    23641 gcctgggggc gttttgatgg aaatcaggaa accctgcaac ccttgttggg gcagttaagc
    23701 tggctggcgg agcaatcctg cgcacatcat catgtgctgg cgctggatag cagagaggag
    23761 atggtttccg ggcagaccac taaacaggtg ctattgctga ataccattcg cacggcgtta
    23821 gatcagggtg atttgctgct ctacgcccag ccaattcgca acaaagaggg tgaaggttat
    23881 gatgagatcc tcgcgcgact gaaatatgac ggcggcatta tgaccccgga taagtttctg
    23941 ccccttattg ctcagtttaa ccttagcgcg cgttttgatt tgcaagtgct ggaatccttg
    24001 ttgaagtggc tggcaacaca cccttgcgac aaaaaaggac cgcgcttttc agtcaattta
    24061 atgccgctca cgctgctgca aaagaatatt gccgggcgga ttattcgtct gtttaagcgt
    24121 tatcacatct ccccgcaggc ggtcattctt gagatcaccg aggagcaggc gttttctaac
    24181 gcagaaagca gcatgtacaa catcgagcag ctgcataagt ttggtttccg gattgcgatt
    24241 gatgactttg gcaccggata tgccaactac gaacggttaa agcgtttgca ggctgatatc
    24301 atcaaaattg atggcgtctt tgtgaaagat attgtcacga atacgctgga tgcgatgatt
    24361 gtgagatcaa ttaccgatct ggcgaaagcg aagtcattga gtgtggtcgc ggagtttgtc
    24421 gagacgcaac agcagcaggc gctattgcat aagctcgggg tgcaatatct gcaagggtat
    24481 ttgattggtc gcccgcagcc attagctgat taacaggcgt aaaaaagccg gggaatgatc
    24541 cccggctttt ttatgtatgg ttttacagca ccagtgctgc gattgacgca gacagcacac
    24601 tcaccagggt agagccgtaa accagtttca gaccgaagcg agaaaccacg ttaccttgct
    24661 cttcattcag gcctttaact gcacctgcga taatcccgat tgaagagaag ttagcgaagg
    24721 aaaccaggaa cacagagatt atgccttcag cacgcggaga gagcgtggaa gcaattttct
    24781 gcagatccat catcgcaacg aactcgttgg aaaccagttt ggtcgccatg atactgccca
    24841 cttgcagtgc ttcactggaa ggaacaccca tcacccatgc aatcggatag aagatgtagc
    24901 ccaggatgcc ctggaaggag atgctgtagc caaaccagcc agtaacggtg gcaaacagtg
    24961 cgttcagcgc ggcgatcagg gcgataaagc cgatcagcat cgcggcaacg ataatggcaa
    25021 ctttgaaacc tgccagaatg tattcaccca gcatttcgaa gaagctctga ccttcgtgca
    25081 ggttggacat ctggatgttt tcttcactgg catcaacacg gtaaggattg atcagcgaca
    25141 gcacgataaa ggtgctgaac atgttcagta ccagcgcagc aacgacgtat ttcggttcca
    25201 gcatggtcat gtatgcacca acgatggaca tcgacacggt ggacattgcc gtggcagcca
    25261 tggtgtacat acgattgcgg gagattttgc cgaggatatc tttataggca ataaagtttt
    25321 cagactgacc cagaatcagg gagctgacgg cgttaaagga ttccagtttg cccatgccgt
    25381 tgactttgga gagcaggaaa ccaattgcgc ggataatcac cggcaacacg cgaatgtgct
    25441 ggagaatacc gatcagtgca gagataaaga cgattgggca cagcactttc aggaagaaga
    25501 atgccaggcc ttgatcattc atgctaccaa agacgaagtt agtcccttcg ttggcaaatc
    25561 cgagcagttt ttcgaacatt tcggagaagc ctttcacgaa gcctaaacca acgtcggagt
    25621 tcaggaagaa ccacgccagt aacacttcga taacaagcag ttgaataaca taacggatac
    25681 gaattttttt gcggtcgctg cttaccagca gtgcgagaat cgcaacaacg gcaagtgcca
    25741 gtacaaaatg aaggacgcgg tccatatttg ctccaaatat gaggcaggtt aaatttccgt
    25801 gcacattcta tgtaacatgg ttaaggaaaa cgagatctga cacacactat aatgacgtcc
    25861 tgtgacaagc ttcaaaaata gtgatctgtc atacatttct gctatgttgt gtatggaagc
    25921 tgaaagttat gtaaatgtgc taacattaaa cataaccata acattcaagt catcaatctt
    25981 ttcgtagtca taatccaggt ctatcagaga aatcaccaca acccatttaa atgaatttga
    26041 taatcattct cgtttggcat agcatgaaac atagcaaagg ctatgtttta gaggcacaag
    26101 atgacgaact atcgcgttga gagtagcagc ggacgggcgg cgcgcaagat gaggctcgca
    26161 ttaatgggac ctgcgttcat tgcggcgatt ggttatatcg atcccggtaa ctttgcgacc
    26221 aatattcagg cgggtgccag cttcggctat cagctactgt gggttgtcgt ttgggccaac
    26281 ctgatggcga tgctgattca gatcctctct gccaaactag ggattgccac cggtaaaaat
    26341 ctggcggagc agattcgcga tcactatccg cgtcccgtag tgtggttcta ttgggttcag
    26401 gcagaaatta ttgcgatggc aaccgacctg gcggaattta ttggcgcggc gatcggtttt
    26461 aaactcattc ttggtgtctc gttgttgcag ggcgcggtgc tgacggggat cgcgactttc
    26521 ctgattttaa tgctgcaacg tcgcgggcaa aaaccgctgg aaaaagtgat tggtgggtta
    26581 ctgttgtttg ttgccgcggc ttacattgtc gagttgattt tttcccagcc taacctggcg
    26641 cagctgggta aaggaatggt gatcccgagt ttacctactt cggaagcggt cttcctggca
    26701 gcaggcgtgt taggggcgac gattatgccg catgtgattt atttgcactc ttcgctcact
    26761 cagcatttac atggcggttc gcgtcaacaa cgttattccg ccaccaaatg ggatgtggct
    26821 atcgccatga cgattgccgg ttttgtcaat ctggcgatga tggctacagc tgcggcggcg
    26881 ttccactttt ctggtcatac tggtgttgcc gatcttgatg aggcttatct gacgctgcaa
    26941 ccgctgttaa gccatgctgc ggcaacggtc tttgggttaa gtctggttgc tgccggactg
    27001 tcctcaacgg tggtggggac actggcgggg caggtggtga tgcagggatt cattcgcttc
    27061 catatcccgc tgtgggtgcg tcgtacagtc accatgttgc cgtcatttat tgtcattctg
    27121 atgggattag atccgacacg gattctggtt atgagtcagg tgctgttaag ttttggtatc
    27181 gccctggcgc tggttccact gctgattttc accagtgaca gcaagttgat gggcgatctg
    27241 gtgaacagca aacgcgtaaa acagacaggc tgggtgattg tagtgctggt cgtggcgctg
    27301 aatatctggt tgttggtggg gacggcgctg ggattgtagt tgaatgagcg tcgcatctgg
    27361 cactattggc ggatgcggcg taaacgcctt atccgcccta cgcgttaaag agccggtttg
    27421 taggcctgat aagactcgct agcgtcgcat caggcattgt gttccattgg cgaatgtgtt
    27481 taatggcggt gtccatgtcc gcggcctttt ccgcgatgat cgtcacgatc gcgccagcct
    27541 tcacgatagc cacgctcata cgctttacgc ttatcccagc cacgatgata gccattatca
    27601 tgacgccacc agcggttttt acgccactca taattgcgat gccagtagtc acggtcgcgc
    27661 cagtgaccac cgtcccagta attaccgtaa tgatcgcgat cgccaatttg taatttgatt
    27721 gatggcagta gggtgatttc acctgcgttt gctgcaagcg gggtaaatgc catcagggcg
    27781 gccgccagaa acagtgacct gaacattgtt attctccttc acgatcgaag ccgtcagcgg
    27841 cctgttaacg caatattacg gggaggtaaa gccccgtatc atcgccataa ctcttaaatc
    27901 ataaaggaga gcattttttg ctaaacgtta tcgtggggtg gctgttgctt cggacgattg
    27961 accatcatca tcggcttacc tgctaataac agccatgccg gaagcatgac gatacagagc
    28021 agcggtagca atgtggtatt gggtacaacg accgccgcca taaaaagact taaccagcca
    28081 tcgcgtgtta ccaccagcac gatgccgaga atagcgcaag aaacggttat tgccgctggt
    28141 acggcgggta cgtgctcatg cagcatcaac cctaatgcca cgccgacaaa cactgccggg
    28201 aagattcgcc caccgcgaaa gccactggcg gcagcaacga ccagggcggc aagtttaatt
    28261 accgccagca aaaagtaatc gctggtgctg aaagcctgat ttgccaccat ctgctgcatc
    28321 tcatccagcc ctttaaacag cgaaactggt ccaccaataa cccccagaat accgagaata
    28381 aatccgccaa tacccagcac gagcaccgga tttttcattt gattcatcat cgcgtgcaac
    28441 cgtggtaagc accatacggc gaccatccct gctgcgatgg caatcgccgc gacaattgca
    28501 ccgctgagaa tatcggtcat ttccatctgt ccgtaatgcg caatgggcag tgaaaaatgg
    28561 ggatggaaaa ataatccggt agtaagtgca ccagctgctg ccgccattaa tggcgcaaaa
    28621 agacgatccc atagcggaac ttcactactg ccatttaagg tttgcgaaaa tatcaacgcc
    28681 gccgcaacag gtgtaccaaa cagcgcgccg atggttccgg cagaggctaa aatagtccac
    28741 tccattcggt tgacgcgcgg taacagacga gcgccaatgg caaccgcaag ggcgatattg
    28801 acggtcatga tcggatgttc cggccccagg ctgacgccgc cagcaagacc gagaattaat
    28861 gctacgataa gtcctggtag cgcagagggc ggaactggtg cgccgatcag cggttcacag
    28921 gcggggtctg gtccggcatg accctggctg aaacggataa ccaaccccac cgcaataccc
    28981 gttagcgtta atacaccgat gatccagaga ggtgaatcct gggctatccc cagagttccc
    29041 ggcagtcgtt gccagagcaa attctgtaat gccaaggcga ttttcatcac cacaatcaga
    29101 ataagactgg acgcaatccc aattgccacg gcggggagcg ataataacaa catggttctg
    29161 gctcgcggat ggagcatgtt ctattcctta tgcggggtca gatacttagt ttgcccagct
    29221 tgcaaaaagg catcgctgca attggtgctg aaacgataaa gtaaatgtgt gacccagatc
    29281 gatatttaca gggagcctgc ctttccggcg ttgttgttat gcccccaggt atttacagtg
    29341 tgagaaagaa ttattttgac tttagcggag cagttgaaga atgacaaagt atgcattagt
    29401 cggtgatgtg ggcggcacca acgcacgtct tgctctgtgt gatattgcca gtggtgaaat
    29461 ctcgcaggct aagacctatt cagggcttga ttaccccagc ctcgaagcgg tcattcgcgt
    29521 ttatcttgaa gaacataagg tcgaggtgaa agacggctgt attgccatcg cttgcccaat
    29581 taccggtgac tgggtggcaa tgaccaacca tacctgggcg ttctcaattg ccgaaatgaa
    29641 aaagaatctc ggttttagcc atctggaaat tattaacgat tttaccgctg tatcgatggc
    29701 gatcccgatg ctgaaaaaag agcatctgat tcagtttggt ggcgcagaac cggtagaagg
    29761 taagcctatt gcggtttacg gtgccggaac ggggcttggg gttgcgcatc tggtccatgt
    29821 cgataagcgt tgggtaagct tgccgggcga aggcggtcac gttgattttg cgccgaatag
    29881 tgaagaagag gccattatcc tcgaaatact gcgtgcggaa attggccatg tttcggcgga
    29941 acgcgtgctt tctggccctg ggctggtgaa tttgtatcgc gcaattgtga aagctgacaa
    30001 ccgcctgcca gaaaatctca agccaaaaga tattaccgaa cgtgcgctgg ctgatagctg
    30061 caccgattgc cgccgcgcgt tgtcgctgtt ttgcgtcatt atgggccgtt ttggcggcaa
    30121 tctggcgctc aatctcggga catttggcgg cgtgtttatt gcgggcggta tcgtgccgcg
    30181 cttccttgag ttcttcaaag cctctggttt ccgtgccgca tttgaagata aagggcgctt
    30241 taaagaatat gtccatgata ttccggtgta tctcatcgtc catgacaatc cgggtcttct
    30301 cggttccggt gcacatttac gccagacctt aggtcacatt ctgtaaatcc ttcctgttat
    30361 atcgggaggt aactctcccg ataatctttt aaatcataca gttaattcaa tttttctttg
    30421 tgtcccctca caaggccgac ctgcgtcaca cttccataca gcgggattaa ttctccagta
    30481 aatgcattat ttgtctggta acggcgattt gttttgcacg ttcataattt cactcgtcaa
    30541 ctatcactga acgaggaatt aacgatgagt aagaaactga ttgccttatg tgcctgcccg
    30601 atgggcctgg ttcacacttt tatggccgct caggcgctgg aagaagcggc ggtagaagcc
    30661 ggttatgaag tgaaaattga aacccagggc gcggacggca tccagaatcg cctgacggcg
    30721 caggatatcg ccgaagcgac catcatcatc cattccgtgg cagttacccc ggaagataac
    30781 gaacgtttcg aatcacgcga cgtttatgaa atcactttgc aggacgcaat taaaaacgct
    30841 gtaggcatca tcaaagaaat cgaagagatg attgcctctg aacaacagta atattcgaca
    30901 gggataattg ttatggccat taaaaaacgc agtgcaaccg ttgtgcctgg cgcatccggt
    30961 gcggcagcgg cagttaagaa tccgcaggcc tctaaaagca gcttctgggg tgaactcccg
    31021 cagcatgtga tgtcagggat ttcacgcatg gtgccgacct taattatggg cggtgtgatc
    31081 ctcgctttca gccagctgat tgcttatagc tggcttaaaa ttcctgctga gatcggcatc
    31141 atggatgctc ttaatagcgg gaaattctcc ggtttcgacc tctccttact taaatttgcc
    31201 tggctgtcgc agtccttcgg cggcgtgttg tttggttttg ccattccgat gtttgctgct
    31261 tttgtggcga actctatcgg tggcaaactg gcgttcccgg ctggttttat cggcgggttg
    31321 atgtctaccc agccgacgca actgctgaac ttcgatccca gcactatgca atgggcgacc
    31381 tcttcgccgg tgccgtcaac cttcattggt gcgctgatta tttctatcgt cgcgggttac
    31441 cttgtgaagt ggatgaacca gaaaatccag ttaccggatt tcctgttagc gttcaaaacc
    31501 acatttttgc taccgattct ttccgccatt tttgtcatgc tggcgatgta ctacgtcatc
    31561 accccctttg gtggctggat caacggcggt atccgtactg tgctgactgc cgcaggtgag
    31621 aagggcgcgc tgatgtacgc aatggggatc gccgcggcga cagcaatcga ccttggtggc
    31681 ccgatcaaca aagccgcagg tttcgttgcc ttcagcttta ccactgacca cgtattaccg
    31741 gtcaccgctc gttctatcgc tatcgttatt cctccgattg gtctgggtct ggcgaccatt
    31801 attgaccgtc gtttaaccgg caaacgcctg ttcaacgctc aactttatcc gcagggtaag
    31861 accgcgatgt tccttgcctt tatggggatt agtgagggtg cgattccgtt tgcgctggaa
    31921 agccccatca ccgccattcc gtcgtatatg gtcggcgcga ttgtcggctc aaccgccgct
    31981 gtctggctgg gcgcagtgca atggttcccg gaatccgcta tctgggcatg gccgctggtc
    32041 actaacctgg gcgtctatat ggcggggatc gcgctggggg cagtcataac tgcgctgatg
    32101 gtggtgttcc tgcgtctgat gatgttccgt aaaggcaaat tgttaatcga tagcctgtaa
    32161 gaaggaaaat ccagatgaca ttactcgctt cgctgcgcga ctggcttaag gcgcaacaac
    32221 tggatgcagt gcttctctcc tcacggcaga acaaacagcc gcatctgggg atctccaccg
    32281 gatcaggcta tgtggttatt agccgtgaaa gtgcgcacat tctggtagat tcgcgctatt
    32341 tcgttgaggt ggaagcccgt gcgcaaggct accagctgca tttgcttgac gcgacgaaca
    32401 cgcttaccac tatcgtcaat caaatcattg ccgatgagca gttgcaaacg ctcggttttg
    32461 aaggccagca ggtgagttgg gaaaccgcgc accgctggaa gtctgaactc aatgcgaaac
    32521 tggttagcgc cacgccggat gtgctgcggc aaatcaaaac gccagaggag gtggagataa
    32581 tccgcctcgc ctgtgggatt gccgatcgcg gagcggagca tattcgccgc tttattcagg
    32641 cggggatgag cgagcgcgag atagccgctg aactggagtg gtttatgcgc cagcagggcg
    32701 cagaaaaagc ctcttttgac accattgtcg ccagcggctg gcgcggggcg ctgccgcacg
    32761 gcaaagccag cgacaagatt gttgcagcgg gcgagtttgt cactctcgat tttggtgcgc
    32821 tgtatcaggg ctactgctct gatatgacgc gcaccttgct ggtgaatggc gaaggggtga
    32881 gcgccgaatc tcacccgctg tttgacgtct atcagattgt cctgcaggca cagctcgcgg
    32941 caatctctgc aattcgcccc ggcgtgcgct gccagcagat tgacgacgcc gcgcgccggg
    33001 tcattaccga ggctggattt ggcgactatt tcggtcataa cactggtcac gctatcggca
    33061 ttgaagtcca tgaagatccg cgtttttcac cgcgggacac cacgacgcta cagccaggca
    33121 tgttactgac cgtggagccg gggatttatt tgccagggca agggggcgtg cgcatcgaag
    33181 atgttgtgct ggttaccccg caaggcgcag aagtgctcta cgccatgccg aaaacagtgt
    33241 tgctcacggg agaggcataa tggatttatc gctattaaaa gcgttgagcg aggcagatgc
    33301 gatcgcctcc tcggaacagg aagtgcggca gatcctgctg gaagaagcgg atcgcctgca
    33361 aaaagaagtg cgatttgatg gtctgggatc ggtgctgatc cgcctcaatg aatcgacagg
    33421 tccgaaggtg atgatctgcg cccatatgga cgaagtggga tttatggtgc gtagcatctc
    33481 ccgcgaaggg gcaattgatg tgctgccggt tggcaacgtg cgcatggctg cccgccagct
    33541 gcagccggtg cgcatcacca cccgtgaaga gtgcaaaatt ccaggcctgc ttgacggcga
    33601 ccggcagggg aatgacgtca gcgccatgcg cgtggacatt ggtgcgcgct cgtatgacga
    33661 agtgatgcag gcgggaattc gtccaggcga tcgcgtcacc tttgatacaa cttttcaggt
    33721 tctccctcac cagcgggtga tggggaaagc ctttgatgat cgcctcggtt gctatctgct
    33781 ggtgacgtta ctacgtgagc tgcacgatgc cgaactgcct gcggaggtgt ggctggtggc
    33841 aagttccagc gaagaggtgg gattacgcgg tgggcaaacc gctacccgca cggtgtcgcc
    33901 ggacgtcgcc attgtgctgg ataccgcctg ctgggcgaaa aactttgatt atggcgcggc
    33961 taaccatcgc cagattggta acggcccgat gctggtgtta agcgacaagt cactgattgc
    34021 gccgccaaaa cttaccgcct ggatcgaaac cgtggcggct gaaattggcg taccgttgca
    34081 ggcagatatg ttcagcaacg gcggcacgga cggcggagcg gtgcacttaa ccggtactgg
    34141 cgtacccaca gtggtaatgg ggcctgccac ccgccacgga cattgcgcgg catcgattgc
    34201 cgattgccgc gacattttgc agatggagca acttttatct gcccttattc aacgtcttac
    34261 gcgtgagacg gttgttcaac tgacggattt cagatgatct cctgattaac ccggagcggt
    34321 tatgttaacg attcaatttc tctgtccttt gcctaacggt ctacatgctc gtccggcgtg
    34381 ggaacttaaa gaacagtgca gccagtggca aagcgaaatc acctttttca accatcgcca
    34441 gaacgcaaag gccgatgcta aaagctcgct ggcgttgatt ggaactggca ccttatttaa
    34501 tgacagttgc agcctgaaca ttagcggcag cgatgaagag caggcgcggc gcgtgctgga
    34561 agagtacatc ctggtgcgct ttatcgacag cgacagcgtt cagccgacgc aggcagaact
    34621 gacggcgcat ccgctgccgc gttcattaag ccgccttaac ccggatttac tgtacggcaa
    34681 tgtgctggca agcggcgtcg gcgtgggtac gctgaccctg ttacaaagcg acagcctcga
    34741 cagttatcgg gcaatccccg ccagtgcgca agattccacc cggctggagc acagcctggc
    34801 aacgcttgcc gagcaactga accagcaatt gcgtgagcgt gacggcgaaa gcaaaactat
    34861 cctcagcgcc catttgtcgc tgattcagga tgatgaattt gcaggcaata tccgtcgcct
    34921 gatgacagaa cagcatcagg gactgggggc ggcgatcatc agcaatatgg agcaggtttg
    34981 cgccaagctt tctgcttctg ccagcgatta tctgcgcgaa cgtgttagcg acattcgcga
    35041 tattagcgaa cagttgctgc atatcacctg gccggaactg aagccgcgca acaatctggt
    35101 gctggaaaaa ccgaccattc tggtggctga agatttaacc ccaagccagt ttttgagcct
    35161 tgatttgaaa aatcttgcgg gcatgattct ggagaaaacc gggcgcacct cgcataccct
    35221 gattctggcc cgcgcctcgg cgatcccggt actgagcggc ctgccgctgg atgcgattgc
    35281 ccgttatgcc gggcaaccgg cggtgcttga cgcccagtgc ggcgtgctgg cgattaaccc
    35341 gaatgacgcg gtgagcggtt attatcaggt tgcgcagacg ctggcggata aacgccaaaa
    35401 acaacaggcg caggctgccg cgcagctggc ctattcccgt gataacaagc gtattgatat
    35461 cgcggcgaat attggcaccg ctctggaagc accaggcgcg tttgccaacg gcgcggaagg
    35521 cgtcgggctg ttccgtaccg aaatgctgta tatggatcgt gacagcgcgc cggacgagca
    35581 ggagcaattt gaagcctacc agcaggtgct actggcagcg ggcgacaagc cgattatctt
    35641 ccgcacaatg gacatcggag gcgataaaag cattccttat ctgaatattc cccaggaaga
    35701 gaacccgttc ctcggctatc gcgcggtacg tatttacccg gaatttgctg gcctgttccg
    35761 cactcaacta cgggcgattt tgcgcgctgc cagttttggc aacgcccagt tgatgatccc
    35821 gatggttcac agcctcgatc agatcttatg ggtgaagggc gagatccaaa aagcgatcgt
    35881 tgagcttaag cgcgatggcc tgcgtcatgc agagacgatt actctgggta tcatggtgga
    35941 agtgccgtcg gtgtgttaca tcatcgacca cttctgcgat gaggtcgatt tcttcagtat
    36001 cggttctaac gatatgaccc agtatctgta tgcggtcgat cgtaataacc cgcgcgtatc
    36061 gccgctgtat aacccgatta cgccatcgtt cctgcgcatg ttgcagcaga ttgttaccac
    36121 tgcgcatcag cgcggcaaat gggtaggtat ttgcggtgaa ctgggcggtg aaagtcgtta
    36181 tctaccgcta ctgcttgggc tgggcctgga tgagctgagt atgagtagcc cgcgtattcc
    36241 ggcggtgaaa agccagcttc gtcaactgga tagcgaggcg tgtcgggaac tggcgtgtca
    36301 ggcatgtgaa tgccgcagtg cgcaggaaat tgaagcgtta ctcaccgcct ttacgccgga
    36361 agaagacgtt cgcccactgc tggcgctgga gaatatcttt gttgatcagt attttagcaa
    36421 taaagagcag gcgatccagt tcctgtgcgg caacctcggc gttaacgggc gtactgaaca
    36481 tccgtttgag ctggaagaag atgtctggca gcgggaagag attgtcacta ccggcgttgg
    36541 ttttggcgta gcgatcccgc acaccaaatc tcagtggatc cgtcattcca gtatcagcat
    36601 tgcccggctg gcgaaaccga ttgactggca gtcagaaatg ggcgaagtcg aactggtgat
    36661 catgctgacg ctgggtgcta acgaagggat gaatcatgtg aaagtcttct cgcagctggc
    36721 gcgtaaactg gtgaataaaa acttccgcca gtcgttgttt gccgcgcaag atgcacaaag
    36781 tatcctgacg ctgctggaaa cagaattaac cttctgacgt tagccctgaa aacgggcgct
    36841 gtactctccc ggcgtcagac caaactgacg ccggaaaacg cgacaaaaat agtcgctatc
    36901 cggaaaaccg caacgctgtg ccacttcatg aattgacaga tgatatttct gcaaaatcat
    36961 cctcgccttc gccattcgca cccaacgcac atactcgata aaacgcatcg ttccatgctg
    37021 agcaaacagt tttgacagat gattgggcgt gatattaaaa aactgcgcca cgctctcgcg
    37081 ggtgagcggc tgggcataat tatcctgaac ccagttacaa atgctgtgat agagaaaatc
    37141 gccgcgcggt tgcgaattac ccggcggcat attcaccacg ttgcggcaaa gatgcagcaa
    37201 acttaacacc agtggctgaa tgatattctg ctccagcggc gaacggctaa gatgggtgag
    37261 ggcggtaagc atcgcttcac cttcaccgcg ttgcaggctg ggaagctgga tctggcgagc
    37321 aggatgcaac aacgacgtgg tgcggctatc gtaaaacgat aacccaagcc aggtcggtgc
    37381 gaacaccagg cttaacagca tcaccggttt gttgttgatc ggtaaattag ccgcgcgtgc
    37441 cggaataaac agcatttctc cttccaccat tcgctgctgc tttgcttcca gcaggttgcc
    37501 gtattcgccg cgcagcacga tatccagacg gggaaaatca atacataaac tgcccaccgg
    37561 taacgaggcc ggctggctgg caaaccagac gcgccccagt agttgcgggt taagcaccag
    37621 gccgctgaac agatcggcga aaaattgttg atcggcgggc aagcctggtg ctttcatgaa
    37681 agttcccgat taaagatgca ttaactggcg aaattctttc actttgctgc gactgaccgg
    37741 cacttcaaaa tccagatctt tcagtcgcag aatgtaggtg ttattaaacc acggttcgat
    37801 ttcgcgtatt ttgttcagat tgacacaaaa tgagcgatgg cagcggaaaa aatgcgacgg
    37861 cggcagtttg ctgcaaaatt cggtaatgtt catcggcatt acgtaggatt cacgccgcgt
    37921 atagacaaag gtcattttct cgtgcgcttc ggcgtaatag atatcgttaa ttggcgtgac
    37981 gattattcgc tcatctttca ccagattaat ggtgtcattc tcacgcgtta ccgtcgcggc
    38041 aggcgtactg ctggtctgct gttgttgcca ggccgcttcc agtttttgca gcatcccggt
    38101 aatacgtgac tcctgatacg gtttgagaat gtagtcaaac gcctccagtt caaacgcttc
    38161 taccgcatgt tctttccacg cggtgatgaa cacaataaac ggtttatggg cgaactggct
    38221 gatgttttgc gccagcaaca cgccatccag cgacggaata ttgatatcca gaaaaatggc
    38281 gtcgacgcgg ttatgctgca aaaacttcaa cacgtccaga ccgtcgtcaa aggtgccgac
    38341 aatctccatc tggctgtgct ctttaattag ccagctcagt tcctgttgtg ccaggaattc
    38401 gtcttcaaca atgatgactt tcacaatatc actccggctc aaagcaataa cgtagcctgt
    38461 gaggcgactg gggtgcgttg gttaggaatg taaaacgcaa tttccgtccc cggctccagg
    38521 cggcggatat gcagcccctc gccatacaat aacttcacgc gatgatggac attcagcagg
    38581 ccaattttat tgcccggcat ttcattcgct tcgacccgct caatcacctt tggatcgatg
    38641 ccgtgcccgg tatcccgcac cgcaatgcgt acccgattgc cgcactctgc aacgctgatg
    38701 gtgacaacgc ctttaccttt gcaaggctga ataccgtgga caatggcatt ctccaccaac
    38761 ggctggatca gcaggctggg aatgcagcaa ttcacctctt catcaatatc atagataacc
    38821 gtcagcttgt cgccaaagcg ggcctgctca atggcaatat agtctttaat ttgatacagc
    38881 tcttttttga tatcgatttg ctcatcgtct tttaattcaa tgttatagcg cagataacgc
    38941 gacagattaa agatcagctg gcgagcagta tccggattca gacggattga cgatgaaata
    39001 gcgttcaaag cgttaaacag gaaatgggga ttaattttgc tttgcagggc gcgaagctct
    39061 gccttatttg ccatttcacg tagctgctcg gcgcgtgaaa cctccagttg ggtggagatg
    39121 atttgcgaca gaccgaccgc catttcctgt aatgacgagg tgatctgatg cgcgtggcag
    39181 tagtaaattt tcagcgttcc ggtcacaacg cctttctccc acaatgggat caccagcatg
    39241 gaatgaatct ctggtgtgcg gtgggcttca tcattgtttt taatgatgat ttttccgtaa
    39301 ttcatcgcct gacgggtagt cgggctaatg aagtcatcgc cattctgata gttatgttca
    39361 cccacgccaa cataggccag cacatgatcg gtattggtaa tcgccaccgc atcggcgtga
    39421 atgtcatcgc aaataatttc acaaactttg cgtaatgact cgctattgac atggcgaaac
    39481 agaggtagcg ttttgttggc gatatccagc gccagcttgg cctgccgcgc ggcgctggcc
    39541 tctttttcgc cctcaacgct ttgcaccaga agcacaataa agccgataca gacgctaccg
    39601 aggatcattg gaatgccgat tttagagacg atatcgatcc ccaacgcggt agttggtgcc
    39661 cagacaatta ccagaatcat ggtcaacgtc tcacacaaca tgccgccgag aataccgacg
    39721 cgccagcgct gtgctttggg gattttcagg tttatccagc ctgatataca acccgccaga
    39781 atgctggtga taaagcaggg gatcgccgtc acgccgccga tatcaattaa ataccggtga
    39841 atacccgcaa tcacgccggt aatgatgcct acccacgggc caaacagaat cccgccggac
    39901 atcaccgcga taatacgtac gttcaccagc gagccttcta cgggaacgcc ggaccaggtg
    39961 ctgaacaggg cgaacagcga gaaaatggcg gtaacggcga gcaattcttt tggggagtgc
    40021 gccgacttgt gcaacagttc gcgaaacagg cggatacgga tgagaaagaa caggcagata
    40081 agcattaacg ctgcccgatc gaagaccgcc agcagcatgt tgaatatttc gtgcacgggt
    40141 gactctgaat aaggattaaa cctctatgat aggtaacctg aaggctgatg accagcaggc
    40201 cgtttttgag gaaaaaccat aaatttaaaa ggggattgaa agataaatgg tgtattagca
    40261 atgtatctct tgataatgca cttctggctg gtaaatttat tgaaattata atgttaataa
    40321 aattggtggt aattttcttg ttagcgtaga aaaatttcct gtttttgaag tggagttcct
    40381 catttaaagc cattgataaa aatgttctaa aacgctggac agacagaaat taatcaggct
    40441 attttcaaaa cagccaccat tgtggcagcg tcgctcggac ggtccgggcg ctaacgttaa
    40501 tctgaggaaa ttatggctga cactcgccct gaacgtcgct ttacgcgcat tgatcgtctc
    40561 ccgccctacg tttttaacat caccgctgag ctgaaaatgg ctgcgcgtcg gcgcggcgaa
    40621 gatattatcg atttcagcat gggtaacccg gacggtgcga ctccgccgca tatcgtcgaa
    40681 aaattatgta ctgtggccca gcgcccggac acgcatggtt actccacttc acgcggcatt
    40741 ccgcggttac gtcgcgccat ttcccgctgg tatcaggatc gctacgacgt tgaaatcgac
    40801 ccggaatcag aagccatcgt cactattggt tcgaaagagg gcctggcgca tctgatgctg
    40861 gcgacgctgg atcatggtga cacggtgctg gtgccgaatc caagttaccc gatccatatt
    40921 tatggcgcgg tgattgccgg ggcgcaggta cgctcagtgc cgctggtgga aggtgtcgat
    40981 ttcttcaacg aactggagcg cgccattcgt gaaagttatc cgaaaccgaa gatgatgatc
    41041 ctcggcttcc cgtcgaatcc aaccgcgcaa tgcgtggagc tggagttctt cgaaaaggtt
    41101 gtggcgctgg cgaaacgcta cgatgtgctg gtggtccacg acctggccta tgccgatatc
    41161 gtctacgatg gctggaaagc gccgtcaatc atgcaggtac cgggtgcacg cgatgtggca
    41221 gtcgagttct ttacgctgtc gaaaagctac aacatggcgg gctggcgtat cggctttatg
    41281 gttggcaaca aaacgctggt cagcgccctg gctcgtatta aaagctatca cgattacggc
    41341 acctttacgc cgttgcaggt ggcggcgatt gcggcgctgg agggcgatca acagtgcgtg
    41401 cgcgacattg ccgaacagta caaacgccgc cgtgatgtat tggttaaagg gctgcatgaa
    41461 gcgggctgga tggttgaaat gccgaaggct tcgatgtatg tctgggcgaa aatcccggaa
    41521 ccatatgcgg ccatgggatc gctggaattt gccaagaagc tccttaacga agcgaaggtt
    41581 tgtgtctcgc cggggattgg ctttggcgac tatggtgata cgcatgttcg ctttgcactg
    41641 attgaaaacc gcgaccgtat tcgtcaggcc atcagaggga ttaaagcgat gttccgtgcc
    41701 gacggtttac tacccgccag cagcaaacat attcacgaaa acgcggaata aacaccgtcg
    41761 tcagaaatgc aaacaggagc ctgatggctc ctgtttttca ttgcacagtt tgcttttaga
    41821 tcatcagggc aaaagtaccg gcccaaacga tgaccataaa agaaatgccc ataaagaaat
    41881 atttcacttt acatctctcc gcgtaattac tgttacgcct gaatgacttc attacacctg
    41941 atacagtgag ttgacaccgg gcaaagcgac cttaaaagcc ctgtatttta ccgtgggcat
    42001 caccccagaa ttctgagcta aactgctcga gacggcgcta atgtacgcct aaaactgaaa
    42061 cgagacaaga ggcattttct tatttacttt tagtaactta caagtgtctc atatcggcga
    42121 cagtttaatt tctgacggta ataattagat attcagcgaa ggaaaaaacc ttaagaaaaa
    42181 agccataaaa accatgaggt tattatggcc gatttgagga gggaaagagt aagagcagtt
    42241 tgttaaatgt acaacgacga ttctcccacc gggcgcgttt taaagcgacg gtggatccag
    42301 aggtactgct ccggtgcgcg catgatctct ttctcgataa tcttgttcat ataggcagcg
    42361 gcttgatttt catctgtcgg gtagccttcc atctctgggg tgatgaacaa acgatatccg
    42421 ctgtaatccg cttttcttac catcgttacg gtcaacatgg ctgcgccaga gagacgggag
    42481 agaacatagg tgccattggt tgtggcgaca ttttccaccg caaagaacgg cgcgaaggag
    42541 ctgcctttac gaccataatc ctgatcggga gcaaaccata ccgcttcacc tttcttcagt
    42601 gcaccgacaa tgccgcgcag attatttctg ccgagcatcg ctttgttaga gcgcatgcgc
    42661 ccacgggtct gcacccattc catcagctga ttattatgtg gacgataggt agccatcatt
    42721 ggttggcaca gtcccatcac gcggccgccc agttccagcg acataaaatg gacgccgaca
    42781 accattacgc cgcgattttg catttgtgcg cgtttaaggt tatccaaccc ttcaacatca
    42841 aaccatttac gtacgcgact gtcgggccag aaccatgcca tgccggtttc taccagcgcc
    42901 atgccgagtg aacgaaagtt ttcggcaatc atcttctcgc gttcttccgc agaatgctgc
    42961 gggaaacaaa gttccaggtt tttacgggcg atagattcac gacgtttcag gaatggtcgc
    43021 gccattgcgc caatacgcgt gccgagaaag cagagaacag gataaggaag ctgtacccaa
    43081 agccagagta caccaagccc aaaccatgtg agccagtagc gaggatgtag aaactcgcgg
    43141 gaaaatttgc attgtggaaa catagttacc ttctgtagat acgaattgtt gtatcgcaga
    43201 agattccatg tcgacagcgg tacactcaat tggttgggcc atcatgtacg acaatggttc
    43261 ctgtaaagag tgtaaagact agtcaggaca taaccacgag gagcatgaat gcggtggctc
    43321 tactttatca ctggctgact gctttcagct actgattatt aatcgtgcaa agtaaaaaat
    43381 tttccgcacg caatagcgtt gcatttaaca tgctgttttc attgatgctt agcaaaaatt
    43441 gccagaggtt aaggattgtc ttactattaa agtgactgaa gttacaggtt ttttagcgcg
    43501 gcaggaggtt gagctatgat ttatttatgg atgtttctcg ccctgtgtat tgtctgcgta
    43561 agcggataca tcggtcaggt actgaacgtg gtatcggcgg tctcttcttt cttcggcatg
    43621 gtgatcctcg ctgcactcat ttattacttc accatgtggt taacaggcgg taatgaactg
    43681 gtgacgggga tatttatgtt tcttgccccg gcttgtggct tgatgattcg ctttatggtg
    43741 gggtatggca ggcggtagtg ggtcaggatg gctcatttgg gcaggtgttc acatcagcat
    43801 cagacatgat tgttcacctg ccaggtgaca gacgctatcg cacaggatga ataagataat
    43861 tcgcctttgt cggaaaaggg ggcgtggttt cttctgtgag gtttaatggt ggcgcatctt
    43921 ctctcatctt aaacaaaatt gcctgtgatg gctgaagttt tgtagagtga gttatcgtca
    43981 tcgctgatgt tttttccgtc tctttatagc tcctctcact tccaccacca gaaatggaaa
    44041 atgcaaaaac attgcatgcc ataatagaga ccaataaaaa taagctaaaa agtattaagt
    44101 ttactttcat agaaagttat cctgaaaatg gttcactgag gacctacg
//
LOCUS       DAPGWD010000024        43712 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPGWD010000024 DAPGWD010000000
VERSION     DAPGWD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.024"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..832
                     /locus_tag="RHX88_001242"
     CDS             <1..832
                     /locus_tag="RHX88_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV2229958.1"
                     /translation="KSESTALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDN
                     IDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYE
                     LQGKRIGMVGYGNISKTLAKRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQS
                     HVIIVLLPFSPELENFISHKYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAAL
                     DVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL
                     "
     gene            complement(911..987)
                     /locus_tag="RHX88_001243"
     tRNA            complement(911..987)
                     /locus_tag="RHX88_001243"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(951..953),aa:Met,seq:cat)
     gene            1196..2293
                     /gene="mltA"
                     /locus_tag="RHX88_001244"
     CDS             1196..2293
                     /gene="mltA"
                     /locus_tag="RHX88_001244"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDV2229959.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            2532..3338
                     /gene="tcdA"
                     /locus_tag="RHX88_001245"
     CDS             2532..3338
                     /gene="tcdA"
                     /locus_tag="RHX88_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDV2229960.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3389..3832)
                     /gene="csdE"
                     /locus_tag="RHX88_001246"
     CDS             complement(3389..3832)
                     /gene="csdE"
                     /locus_tag="RHX88_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDV2229961.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQALSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(3832..5037)
                     /gene="csdA"
                     /locus_tag="RHX88_001247"
     CDS             complement(3832..5037)
                     /gene="csdA"
                     /locus_tag="RHX88_001247"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDV2229962.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5229..5456
                     /locus_tag="RHX88_001248"
     CDS             5229..5456
                     /locus_tag="RHX88_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDV2229963.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            5807..6724
                     /gene="gcvA"
                     /locus_tag="RHX88_001249"
     CDS             5807..6724
                     /gene="gcvA"
                     /locus_tag="RHX88_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDV2229964.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            6743..7138
                     /locus_tag="RHX88_001250"
     CDS             6743..7138
                     /locus_tag="RHX88_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDV2229965.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            7131..8231
                     /gene="rlmM"
                     /locus_tag="RHX88_001251"
     CDS             7131..8231
                     /gene="rlmM"
                     /locus_tag="RHX88_001251"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDV2229966.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8275..9006)
                     /gene="fucR"
                     /locus_tag="RHX88_001252"
     CDS             complement(8275..9006)
                     /gene="fucR"
                     /locus_tag="RHX88_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDV2229967.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVAASLVTA"
     gene            complement(9064..9486)
                     /gene="fucU"
                     /locus_tag="RHX88_001253"
     CDS             complement(9064..9486)
                     /gene="fucU"
                     /locus_tag="RHX88_001253"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDV2229968.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(9488..10906)
                     /gene="fucK"
                     /locus_tag="RHX88_001254"
     CDS             complement(9488..10906)
                     /gene="fucK"
                     /locus_tag="RHX88_001254"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDV2229969.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(11015..12790)
                     /gene="fucI"
                     /locus_tag="RHX88_001255"
     CDS             complement(11015..12790)
                     /gene="fucI"
                     /locus_tag="RHX88_001255"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDV2229970.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKKFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(12823..14139)
                     /gene="fucP"
                     /locus_tag="RHX88_001256"
     CDS             complement(12823..14139)
                     /gene="fucP"
                     /locus_tag="RHX88_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV2229971.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            14686..15333
                     /gene="fucA"
                     /locus_tag="RHX88_001257"
     CDS             14686..15333
                     /gene="fucA"
                     /locus_tag="RHX88_001257"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDV2229972.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            15361..16509
                     /gene="fucO"
                     /locus_tag="RHX88_001258"
     CDS             15361..16509
                     /gene="fucO"
                     /locus_tag="RHX88_001258"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDV2229973.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPTLAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(16564..17319)
                     /gene="xni"
                     /locus_tag="RHX88_001259"
     CDS             complement(16564..17319)
                     /gene="xni"
                     /locus_tag="RHX88_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDV2229974.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYGNLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(17431..18798)
                     /gene="sdaB"
                     /locus_tag="RHX88_001260"
     CDS             complement(17431..18798)
                     /gene="sdaB"
                     /locus_tag="RHX88_001260"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDV2229975.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(18856..20145)
                     /gene="sdaC"
                     /locus_tag="RHX88_001261"
     CDS             complement(18856..20145)
                     /gene="sdaC"
                     /locus_tag="RHX88_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDV2229976.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(20702..22066)
                     /gene="ppnN"
                     /locus_tag="RHX88_001262"
                     /gene_synonym="ygdH"
     CDS             complement(20702..22066)
                     /gene="ppnN"
                     /locus_tag="RHX88_001262"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDV2229977.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(22178..23026)
                     /gene="queF"
                     /locus_tag="RHX88_001263"
     CDS             complement(22178..23026)
                     /gene="queF"
                     /locus_tag="RHX88_001263"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDV2229978.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            23094..23639
                     /gene="syd"
                     /locus_tag="RHX88_001264"
     CDS             23094..23639
                     /gene="syd"
                     /locus_tag="RHX88_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDV2229979.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            24261..24590
                     /locus_tag="RHX88_001265"
     CDS             24261..24590
                     /locus_tag="RHX88_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDV2229980.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            24590..25372
                     /gene="truC"
                     /locus_tag="RHX88_001266"
     CDS             24590..25372
                     /gene="truC"
                     /locus_tag="RHX88_001266"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDV2229981.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            25390..25839
                     /locus_tag="RHX88_001267"
     CDS             25390..25839
                     /locus_tag="RHX88_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDV2229982.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            26274..27626
                     /gene="gudP"
                     /locus_tag="RHX88_001268"
     CDS             26274..27626
                     /gene="gudP"
                     /locus_tag="RHX88_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDV2229983.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            27628..28968
                     /locus_tag="RHX88_001269"
     CDS             27628..28968
                     /locus_tag="RHX88_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV2229984.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            28989..30329
                     /gene="gudD"
                     /locus_tag="RHX88_001270"
     CDS             28989..30329
                     /gene="gudD"
                     /locus_tag="RHX88_001270"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV2229985.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGRTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(30561..33317)
                     /gene="barA"
                     /locus_tag="RHX88_001271"
     CDS             complement(30561..33317)
                     /gene="barA"
                     /locus_tag="RHX88_001271"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDV2229986.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            33374..34675
                     /gene="rlmD"
                     /locus_tag="RHX88_001272"
     CDS             33374..34675
                     /gene="rlmD"
                     /locus_tag="RHX88_001272"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDV2229987.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            34723..36957
                     /locus_tag="RHX88_001273"
     CDS             34723..36957
                     /locus_tag="RHX88_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDV2229988.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            37035..37283
                     /gene="mazE"
                     /locus_tag="RHX88_001274"
     CDS             37035..37283
                     /gene="mazE"
                     /locus_tag="RHX88_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDV2229989.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            37283..37618
                     /gene="mazF"
                     /locus_tag="RHX88_001275"
     CDS             37283..37618
                     /gene="mazF"
                     /locus_tag="RHX88_001275"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDV2229990.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            37689..38480
                     /gene="mazG"
                     /locus_tag="RHX88_001276"
     CDS             37689..38480
                     /gene="mazG"
                     /locus_tag="RHX88_001276"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDV2229991.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            38708..40345
                     /gene="pyrG"
                     /locus_tag="RHX88_001277"
     CDS             38708..40345
                     /gene="pyrG"
                     /locus_tag="RHX88_001277"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDV2229992.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            40433..41731
                     /gene="eno"
                     /locus_tag="RHX88_001278"
     CDS             40433..41731
                     /gene="eno"
                     /locus_tag="RHX88_001278"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDV2229993.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(41787..42149)
                     /locus_tag="RHX88_001279"
     CDS             complement(41787..42149)
                     /locus_tag="RHX88_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229994.1"
                     /translation="MSKILANHPRLSKFVFGLFGAAGMIWLNKAFQGDELFQLITSLL
                     ALDTFVYFCIYSLINPIPEANDFLWDYKVHQRKYSSNISHNDFVSNVRNSRKIVRILI
                     LVGITFINVIALLTKLKM"
     gene            complement(42185..43090)
                     /locus_tag="RHX88_001280"
     CDS             complement(42185..43090)
                     /locus_tag="RHX88_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDV2229995.1"
                     /translation="MKNLILLLVLICSSAFAVQVPVPEFAKYINDLTGTLTREEVSTL
                     TSQIKTLTQKSHAQLIVLVVETTGDETIEQYATRVFERWQPGHKNLDDGILLVVAWQD
                     HTVHIEIGYGLEGILTDAQSGQIIRNSIIPAFKKGDLAVGLQQGIGDIETRLSENDLV
                     NNTSPDHPLPFSGWWALLVWAIVLTFISARGYVKTLGVICFAAIILAFVLPIAGYSGS
                     WGVLATLLCFATPFLVVAIAFTPFGRKVRDSMRSANRPSRYERSNSASRHSSSSSSSS
                     SSSFNNDNFSGGGGSSGGGGASGRW"
     gene            complement(43104..43706)
                     /locus_tag="RHX88_001281"
     CDS             complement(43104..43706)
                     /locus_tag="RHX88_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDV2229996.1"
                     /translation="MLRTFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRA
                     DLIPNLVASIKGYSNHEKDVLEAVTLARSQANRASSDLQQTPGNEQKLQAWQQAQAQV
                     TRTLGQLTIISERYPELKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNASAH"
BASE COUNT        10912 a        10524 c        11506 g        10770 t
ORIGIN      
        1 taaatcagaa tccactgcat tacgagaatc tatttgcgcg gtcatcgccg gaaaagattg
       61 ttatcaggcg gacacgctat cattgctgcc tggtgtgaaa ataatatcga gattcgggac
      121 ggggatagat aatattgatc ttcgtgcagc acaacaatcc ggtattgtag tcaataatgc
      181 ggttgggatt aattcaaatg ctgtcgcgga atttatcatt ggacttatct tcgcgagcat
      241 gagaaatatt ccaggcagct atcatgcgat gcaaaatggc tactggggtg agtcgcatgg
      301 ctatgaatta caaggcaaac ggattggcat ggttggctac ggcaatataa gtaaaaccct
      361 ggcaaaaagg ttatccggtt ttgatgttga gcttttagct tatgacaaac aacccgatta
      421 tcaggttgct gacaaagttg gagttcagtt tgtatcaatt gaagatatct ttatgcagtc
      481 gcatgtcatc attgttcttt tgcctttttc tcctgagctg gagaacttca ttagtcataa
      541 atatttgtcg atgatgcgca atggtgctct aatcatcaat gccgcaaggg gaaagttgct
      601 ggatgagggc gctttactac aggtgattga agaacgaaat gtgtttgcgg cgctggatgt
      661 gtttagctct gaaccgttgg cgcaatttag cccactgcta catgccaaaa atatcattac
      721 aacgccgcat attgcggccg ccaccgttga gtcataccag caaaccggta cacacgttgc
      781 ccagtcgata attgattact tcgcaggaag ggagataaaa aacgtgttgt gagttcagcg
      841 aacgaagttt ttaaatacaa aaaaaccgca atttgtggaa cacattgcgg tttatcagca
      901 ttctaagtat tggttgcggg ggccggattt gaaccgacga ccttcgggtt atgagcccga
      961 cgagctacca ggctgctcca ccccgcgtcc gtggatgcgc actatactcc gctcgcgttt
     1021 tcatgcaacc ttttttttac cccgatcacg gatctggcat gattttgaac aaaaacaaat
     1081 cactaaagcc tattttttgt gcaaaatttg cgtcagggag aggctgctca tttcattaaa
     1141 cgcggcggtt tgttatcttc gttgcgcctt attttttaac ctgaagaaga gaacaatgaa
     1201 aggacgttgg gtaaagtacc ttcttatggg cacggttgtg gcaatgcttg ccgcctgctc
     1261 ttccaaacca accgatcgcg gacagcaata taaagacggg aaatttaccc agcctttctc
     1321 tctggtgaac cagccagatg ccgttggcgc gccgattaac gccggtgatt ttgccgagca
     1381 aattaaccat atccgtaatt cgtcaccgcg tctgtatggc aaccagagta atgtttataa
     1441 cgcggtgcaa gagtggctgc gcgcaggcgg tgatacccgc aatatgcgtc agttcggcat
     1501 tgatgcctgg cagatggaag gtgccgacaa ctatggtaac gtgcagttta caggctatta
     1561 cacgccggta attcaggcgc gccatacccg ccagggcgag ttccagtatc cgatttaccg
     1621 tatgccgcca aaacgtggtc gtctgccgtc tcgtgcggag atctacgcag gggcgctgag
     1681 tgataaatat attctcgctt acagcaactc cctgatggat aacttcatta tggatgtgca
     1741 gggtagtggg tatatcgact ttggtgatgg cagtccgctt aactttttca gctatgcagg
     1801 gaaaaacggt catgcctatc gcagcattgg taaggtgctg atcgaccgtg gcgaagtgaa
     1861 aaaagaagac atgtcgatgc aggcgattcg tcactggggc gaaacacaca gtgaagccga
     1921 ggttcgcgag ctgctggaac agaacccgtc tttcgtcttc tttaaaccgc aatcttttgc
     1981 accggtgaaa ggggcaagtg cggtgccgct ggttggccgt gcttctgttg cctccgatcg
     2041 ttcaattatt ccgccaggta caaccttgct ggcagaagtg ccgctgctgg ataataacgg
     2101 caaatttaac ggtcagtacg aattgcgtct gatggtggcg ttggatgtcg ggggcgcaat
     2161 caaaggccaa cacttcgata tctatcaagg gatcgggccg gaagccggac accgcgcagg
     2221 ttggtacaac cactatggac gtgtctgggt gctgaaaacc gccccgggcg caggtaacgt
     2281 ctttagcggc tgatgtgata ttctgtatgc cgtttttacg gatatgacag ggtgaggggt
     2341 aacctcacat cctgattatt gactaagtgc gcgttgttca tgccggatgc ggcgtgaacg
     2401 ccttatccgg cctacaaatc atgcaaatcc aaaatattgc aggagttatg taggcctgat
     2461 aagcgtagcg catcaggcat ttatgcgttt agggtgaggg ataacctcgc cctttttaat
     2521 tctgaggtgc tatgtctgtg gtaattagtg atgcatggcg tcagcgtttt ggtggcacag
     2581 cgcgtctgta tggtgaaaaa gcgttgcaac tgtttgctga cgcgcatatt tgtgtggttg
     2641 gtattggcgg tgtcggttcc tgggcggcgg aagcgctggc gcgcacgggg attggcgcaa
     2701 tcacgcttat cgatatggat gatgtgtgcg tcaccaatac caatcggcaa attcatgccc
     2761 tgcgcgataa cgttgggctg gcaaaagcgg aagtcatggc ggaacgtatt cgccagatta
     2821 acccggagtg ccgtgtaacg gtggtggatg atttcgtgac gccggataac gtagcgcagt
     2881 atatgagtgc cggttattcg tacgtgattg atgccattga tagtgtacga cccaaagcgg
     2941 cgctgattgc ttattgtcgg cgcaataaaa tcccgctggt cacgaccggt ggcgcgggtg
     3001 ggcagattga tccgacgcag attcaggtta ccgatctggc gaaaacgatt caggacccgc
     3061 tggcggcgaa gttgcgcgag cgcctgaaaa gcgattttgg cgtagtgaaa aacagtaaag
     3121 gcaagctcgg cgtggattgc gtgttctcta ccgaagcatt ggtgtacccg cagtcagacg
     3181 gcacggtctg tgcaatgaaa gccacggcag aagggccgaa gcggatggat tgtgcatctg
     3241 gatttggtgc ggcgacgatg gtgaccgcca cctttggttt tgttgcggtt tctcatgcgc
     3301 tgaagaagat gatggcgaaa gcggcgcgtc agggttaaac aatcaggtcg gatgcgacgc
     3361 gaacgtctta tccgacctgg ttctgtgctt aaacctgctt cgccgcagcg ataatcgcct
     3421 cgcttaacgc attcaacccc tggctgcgtg aggcgctaag ctgcgcacgt aatcccagct
     3481 catcaaacaa tgccagcggc gacagtgcct gcaattcggc ggcggttttc ccctcaacgg
     3541 cagtcaacag caccgccagc aggccgcgca ctatacgtcc ttcgctgtcg ccaaagaagt
     3601 gcattttacc gttttcagcc acggtatatc ccagccagac gcggttttcg catccggcaa
     3661 tctctttagc ctgcgctttt aactcgtctg gcaaagccgg aagctgtttc cccagcatga
     3721 tcaactggcg atatttatct tcccattgcg tcaacggtgc gaaggtattg cgtaacgttt
     3781 ctgcggttac ggttgtgccg aacggatgtc cggcgaattg cgggttcgtc attaatccac
     3841 caataattcc agcgcgcggt caacggcatt caccagcgca tccacatcac tctttgtatt
     3901 atatggcgca aaagaggcgc gcagtgtgcc ggttacgcct aattctgcca gtagcggctg
     3961 agcgcaatgt tgcccggccc gcaaggcaat accgtactcc gccagcagcg tcaccatatc
     4021 gctgtgatga acgccagcaa aatcaaaggc cagcaggctg gaatcctggc agcggaatga
     4081 acggaagcct ggacgtttcg ccagcgcatc ttccgccagt gttgctaagc tacggctcca
     4141 gttttcggcc tggttgatat cgtaatctgc cagccattcc agcgccgcgc ttaatcctat
     4201 gacaccagcc acatttggcg ttcctgcttc cagtttccac ggcgcagatt gagtcgtgaa
     4261 gccgtcaaaa ctcacttcgt gaaccatttt gccgccgccc agccagggcg acatcgcttc
     4321 cagcagttct gatttgccat acagcacgcc gatacctgtc gggccataca gtttgtgacc
     4381 tgaaaaagca tagaaatcaa tattcagttg ctgaacatcc gcggggaaat gcaccgcccc
     4441 ctgagcacca tcaaccatca ccaccatccc cgctgaatga gcaaaggtaa tcgctcgcgc
     4501 cagatccggg caaccgccag taacgttcga catctgaccc aacgccagaa tccgactacg
     4561 gggagtaatc agttctggca acaaatcgac atccggcagt cgctgcgcat taagcggcaa
     4621 tttcaccact ttggctccag tttgttgggc gaccatcagc caggggacga ggttggcgtg
     4681 gtgttctgcc acgctgacaa taatctcatc gcccggttgc agacgcggac gcgcatagca
     4741 ttgtgcaacc atgttgatgg attcagtggt gccgcgcgtc cagacgatag ttttatcatc
     4801 cggtgcattc agtaattgcg ccactttctc tcgtgcagct tcataacgcg cggtcaggcg
     4861 ttgggcttcg gcaaactggc tgcgatggac gtttccggcg ctcaggctgt aaaactgtcg
     4921 ggtggcttca accacggctt caggtttaag cgcggtcgcg gcgctgtcga gatagacgcc
     4981 cgcatcctgt agagcgggaa actgggcgcg aaactgcgcg ggattaaaaa cgttcatggt
     5041 actcctcggc ttgatgcacc gatcgtgacg caaaattcac tttgatacaa ggagagtgct
     5101 aaccatcaga attatctgga tatcctggcg aagccgcgta agtaacatat gctgaatagt
     5161 gttaggcgtt aattttagcc tgttaatcaa tactattaag atttattaat cgctttaagg
     5221 agaagatgat gaaaaagact gccgcaatta tttctgcctg tatgctgact tttgccctga
     5281 gcgcctgttc cggttcgaac tatgtgatgc acaccaatga cggacgtacc atcgtctctg
     5341 acggcaaacc acagactgat aacgataccg gtatgatttc gtataaagac gctaatggca
     5401 acaaacagca gatcaaccgt actgacgtga aagagatggt cgaactggat cagtaattca
     5461 cgatcgcaag gtaaaaaaaa gcaccgcaat taggcggtgc tacattaatc actatggaca
     5521 gacagggtaa atgtacagga agtgaaaaaa ggtagctttg ctaccatggt ctgaatcgca
     5581 gaccaattgc aaacacaaca acacaacatc acaaccgtaa gccaaaagtt caccagaaca
     5641 cgcattccga taaaactttt cgttccggct caggaagtgc cgccactata ggtatttgct
     5701 ggtagaagct caacggacaa tttataatgg ctcagattaa aaaaactaat aggttacata
     5761 gtgtgatcta attgttaaat tcatttaaca tcaaagttta aaagccatgt ctaaacgatt
     5821 accaccgcta aatgccttac gagtttttga tgccgcagca cgccatttaa gtttcactcg
     5881 cgcagcagaa gagctttttg tgacccaggc cgcagtaagt catcaaatca agtctcttga
     5941 ggattttctg gggctaaaac tgttccgccg ccgtaatcgt tcactcctgc tgaccgagga
     6001 aggtcaaagc tatttcctcg atatcaaaga gatattttcg caattaaccg aagcgacgcg
     6061 taaactccag gcccgtagcg ccaagggggc gttgacggtc agtttactcc ccagtttcgc
     6121 cattcattgg ttggttccgc gactttccag ctttaattca gcttatccgg gaattgacgt
     6181 tcgaatccag gcggttgacc gtcaggaaga taagctggcg gatgatgttg atgtggcaat
     6241 attttatggt cggggcaact ggccggggct acgggtggaa aaactttacg ccgaatattt
     6301 attgccggtg tgttcgccgc tactgctgac tggcgaaaaa cccttgaaga ccccggaaga
     6361 tctggctaaa catacgctat tacatgatgc gtcacgccgt gactggcaga catatacccg
     6421 acaattaggg ttaaatcaca tcaacgttca gcaagggcca atttttagcc atagcgccat
     6481 ggtgctgcaa gcggctattc acgggcaggg agtggcgctg gcaaataacg tgatggcgca
     6541 atctgaaatc gaggccggac gtcttgtttg cccgtttaat gatgttctgg tcagtaaaaa
     6601 cgctttttat ctggtttgtc atgacagcca ggcagaactg ggtaaaatag ccgcctttcg
     6661 ccaatggatc ctggcgaaag ccgctgctga acaagaaaaa ttccgctttc gttatgaaca
     6721 ataatttacg tagggtacga ccatgaccag ccgttttatg ctgattttcg ccgccattag
     6781 cggcttcatt tttgtggcac tgggcgcttt tggcgcgcat gtgttaagta aaaccatggg
     6841 ggccgttgag atgggctgga tccagaccgg cctcgaatac caggcgtttc atacgctggc
     6901 gatcttaggt ctggcggtgg caatgcagcg tcgcatcagt atctggtttt actggagtag
     6961 cgttttcctc gcgttaggca cggtgttgtt cagcggcagc ctttattgcc tggcgctgtc
     7021 ccatctgcgt ttgtgggcat ttgtcacacc ggttggcggc gtgagcttcc tcgcgggctg
     7081 ggcgttaatg ttagttggtg ctatccgttt aaagcgcaag ggcgtaagtc atgaataagg
     7141 ttgtattgct gtgccgtccg ggctttgaaa aagagtgtgc cgcagaaatt accgataaag
     7201 ccggccagcg ggaaattttc ggttttgccc gcgtgaaaga gaatgcgggt tatgtcattt
     7261 atgaatgtta tcaacctgat gatggcgata agttaatccg tgagctgccg ttcagttcat
     7321 taatttttgc ccgccagtgg tttgtggtgg gggaactcct gcagcatttg ccgccagaag
     7381 atcgtattac ccccattgtc ggcatgttac agggcgtagt agagaagggc ggtgaactgc
     7441 gtgttgaagt tgccgatacc aacgaaagca aagagttgct gaaattctgc cgtaaattta
     7501 ctgttccgct acgcgctgcc ttgcgcgatg ctggggtgct ggcgaactat gaaacgccga
     7561 agcgtccggt tgtgcatgta ttctttattg caccaggatg ctgctatacc ggttactcat
     7621 acagcaacaa taattcgccg ttctatatgg gcattccgcg cctgaaattt ccggcagatg
     7681 cgccgagtcg ttccacgctc aaactggaag aggcatttca tgtgtttatt cctgcggatg
     7741 agtgggatga acgcctggcg aacgggatgt gggcggtgga tttaggcgct tgcccaggcg
     7801 gctggaccta ccaactggtg aagcgcaaca tgtgggttta ttccgtcgac aacggcccga
     7861 tggcgcaaag tctgatggat accggacagg tgacgtggct gcgggaagac ggtttcaaat
     7921 tccgtccgac gcgcagcaat atctcctgga tggtatgcga tatggttgaa aaaccggcga
     7981 aagtagcggc attgatggcg cagtggctgg ttaatggctg gtgccgtgaa accattttca
     8041 acctcaaact gccgatgaaa aaacgctacg aagaagtgtc acacaatctg gcgtatattc
     8101 aggcacagct tgatgaacat ggcataaatg ctcagattca ggcgcggcag ttatatcacg
     8161 atcgcgaaga agtgacggtg cacgtccgcc gtatctgggc tgcggtgggt ggtcgtcgcg
     8221 acgagcgata acaggcaagg ccggggcgtc gtctccggcc tgctaccctt tccgtcaggc
     8281 tgttaccaaa gaagccgcaa cctggcgctc atcagaaata atgtgcgtga cctcatccag
     8341 atgcccgatg cgggcttccc ctgaacgatt aaatttactt ttatcaatca ataacaacga
     8401 ttgcgcggca cgttttaata gcattgattt gtaatcagcg ttgatggcat tggagtccca
     8461 cagtgcgccg ctgctatcga tcccttcaca agaaaaaata aacagatcga tttccagcga
     8521 tttaagttgg gaaatcagcg agggattgac gtaacagcca tatttgcgct caagtgtgcc
     8581 gccggaactg attagttgaa tgcgttcgcg tttaccgagt tcatggcaaa tcggatggct
     8641 attggtgaag acctggatgt tgatgtcagg caactggcgt gccagatacc agcaggttga
     8701 actggcatct aaggctatca ccatcccttc ttcaatccac gcgagcgcct cgcgcgcgat
     8761 atctgcttta tgcgcataat ggcttttcag ccggatgtga aagggatcgc cactgtcttg
     8821 attttgacgg tggatatatt tagcgcgccc atgattgcgc agaattttac cctgcgtctg
     8881 taattcattg agatcgcgac gaatggtttc tttactgacc tttagctgtt cagagagagc
     8941 ttccgtggtc aggctggtat ggttcagcag caggtcgact atcgcttgct ggcgtgccgc
     9001 tttcatattt ttcaccccat cattcgataa ccgcccgcgt catgcgggcg taccggtatg
     9061 agattacggt gttacccctt tttttaaaag aatattcccg tacttcgctc gttcgcctgt
     9121 gataacgatc gcaaaggctt tttgcgcccg ttcataaaac gcaaaacgat tgatgcggat
     9181 gatgtcagga catggggctt gtagtgaaag cgcattacgg taacgtcgtt ctacttcagg
     9241 atcgagagtg tcaccttcta ccgccgccat catcaccagt ggcggggcat aactgtccag
     9301 ttcaaataac gggataatcg cctggagcaa gtcgctcacc aacaggccat cagcgcggat
     9361 cacctgcggt cccatcgaat gggcgggaaa gtgagcatcg gaaaaaataa tttcatctcc
     9421 atgtcccatc tctgccagca ctttcaatag ttcgggagaa attaacggcg aaattgtttt
     9481 cagcatttca cacttcctct ataaattcag gttcagtttg cgggtagaaa taacggtact
     9541 gataatgaat ctgtgcacgg gcttcttccg ggctgttaaa ttcccctacg ccataccaac
     9601 cgaacagcgc agctcctgca acggtcgttt cggcatcgtc gagaactttt accggaatat
     9661 caagcatatt ggctttaatc tgattccaca atgtgttgcg acttcctcca ccgactaaca
     9721 ataattcaga ggctttaaag tgcccgattt tttccagcat ctgtagattg cgctgtaatt
     9781 gcgcagttaa cccttccagc gccgcgcgat agaaatgccc ccgcgtggta ttaagcgtca
     9841 ctccttgcca gccagcgttc tgacacgaca ataaatcaca ctgcattttc acaccatccg
     9901 cgccaggtgc gatcagacga gcttcttcaa tcaacatttg ccagggtgtt tcagccgtcc
     9961 agaacagttt tctcacccat tccagcacgc cggatgccag ccattgcata cctgggttat
    10021 acaaccctgc ctggctatcc agttcgcagg tggaaccggc gtactgactt aacagcgaag
    10081 tatcaacctg ggcgctacga accattaaaa tttcccatgt accggaagag agcacgggtt
    10141 cattttggtc ggcaccagcg ccaaaaaggg cgaactgggt atcgtgacct gcggaaatca
    10201 ccggtatgcc aacgggtaag ccgagcattg ctgcggcgct gttctgtagc gtaccaatct
    10261 gttcacccgc ttccaccaga cgagggaaga gtcggcgtgg aataccggtg gcttgtaaaa
    10321 tttgcggact gaaatcgcgt tgctggatat ccagcatctg gctggttccg gccatcgtga
    10381 tatcagtagt gaattcgccg gttaaacggt ggttaatcag cgacgaaata aagagccagg
    10441 cgtgcgcgcg ttccagcagt tgtggatgat tttctttcaa ccacaccaac ttatataacg
    10501 tattgaaact aaaggctccg acgccagaaa tagcctgcaa ccgctgtgcg gagatcaacc
    10561 gttcaatatt gtccataacc gctgctgttc gcggacattt ccagctaatg atcggataga
    10621 gcagattgcc ttgcttatct accagagcgc catccacacc aaaggtggtg acggcgatac
    10681 cgcggatgtg gcattcagtc agttcactat tgatttgccg acagcaatca gcaaagcgtt
    10741 gcaaaatggc gtctaaagac cactggtgcc aggtgttgtt ttccatcgcg atatcgctgg
    10801 cattaggcgt tgaggcgcgg gcaacaattt ttccctgccg attaaccgcg atggccctga
    10861 cattggtcgc gccacagtcg agtaccagga taacttcttg tttcataatc gctcctgcaa
    10921 tatagccgga taacattgct tatccggcta accactcttg cttgttcagg cccggtagcc
    10981 ggagcgaccg ggcatcacat cagggagtaa tgtattaacg cttgtacaac gggccgtagt
    11041 tctggcaagc gcggtaatcc tgaccttcaa tatccatgcc gtgcgcggcc caggcagaag
    11101 gacgatatac tttggtctct tcaacgttgt gcatacatac cgggatacgc agcatggagg
    11161 cgagagtgat aaagtctgcg ccaacgtggc cgatggtcag aaccccgtgg ttagcgcccc
    11221 agttcgccat taccgaatac acatccgtaa acggcccttt accggtgagg cgcggtgcaa
    11281 accaggtggt tggccaggtt gagttggtgc gtttgttgag gatgtcatgc acatccttcg
    11341 gcaattccac gctccagcct tccgcgattt gcagtaccgg tcccaggcct ttgatgatgt
    11401 tgacacgggt catggtgaac gggacgccgc cttcggtaag gaagcgggaa gagtaaccgc
    11461 cgccacggaa gtattcgtgg atcgccgggc accattcggt agcggcgagg caagcgtcag
    11521 cctcttgctg agagatttcc cagtgcggct tcatcgtcgg gttaccttcg ctgtcgcgtt
    11581 gtttacagga accgtccagc gcagcagaac ctgagttgat caaatggatg atgccgtgtt
    11641 ctgccagtcc atcgagttta tgccccgtta cacgctcaat tgcttctggt gaccagtagg
    11701 tacgcacatc ggcaaatacc tgagcggtgc cggtgagctg gtgacccatt agcattgcca
    11761 cgccgttaag actgtcgttt tcagtcgcca cgacaaaggg ttcgcgcacg ccattccagt
    11821 caaatgaact gttgaggatc gcttcggcgg tgtcaccatt gggatattga tcggtccagt
    11881 gacgttgccc ctggaagccc gcagcgatgg cgttgtagcc aagtgattct tccacgcgac
    11941 caatatcggc cagtttgctg ttgccttgca tcatgtcgcg gatacacatc gccatcagta
    12001 aactttcgcg cagaactgcg cggctttgct cggcattgcg ttgatactgt ttgttatttt
    12061 catcttcgcc atagcggaat tttttatcag cccaggccag tgccatttcc agttcggctt
    12121 cgtcgtaaat cttctgatcg atacggcgac gcagttcggt catatccacc gcctggactt
    12181 tcattcccag ccaggattca aagaagttgt gatcaacaat agaaccggcg atacccatcg
    12241 aaacgccgcc cagcgacaga tagcttttac ctttcatgct ggcgacggcc aaaccggcgc
    12301 gggcaaagcg cagcagtttt tcttcaacat cggcaggaat cgatgtgtca tcggcatcct
    12361 gaacgtcatg accgtaaatg gagaatgccg ggatgccttt ctggctgtga gctgccagag
    12421 ccgctgccag gtaaacagcg ccgggacgtt cagtgccgtt aaagccccaa atggctttcg
    12481 ggcgggttgg atccatgtcg atggtttcac tgccatagca ccagcaaggc gttaccgtaa
    12541 tggtgaggcc tacattctga ctgctgaatt tttcttcgca agcagcggct tcagccatac
    12601 ccgcgataca ggtatcggaa atgacacact cgacggcagc tccgcaggca tggcgcagtt
    12661 tctcggtcag cagtgcggcc gtagctttcg ccatattcat tgtttgttct tcaagcgact
    12721 cacgaacacc catgcgacga ccgtcaataa ccgggcggat accaattttc ggtaagctga
    12781 tttttttcat tacagattcc tcactttatt cggaaaatat gttcagttag ttgccgtttg
    12841 agaacggaaa cgggcaaaga taaagatgac cgcgaagcag agtgcgggga tcagttcagc
    12901 tgtggggatg ttgcccgcag cgtcactgac aaaacccatg accggagtga caataccgcc
    12961 gccaataatg gtcataacga tgaaggacga accgtatttg gtgtcctggc cgagattctt
    13021 aatgcccagc gagaagattg ttgggtactg aatcgacata aaggcgctgc ataaagtcag
    13081 ggctattaag cccacatgac cgccagcgaa ggctgagatc aggcacagtg ccatagcgat
    13141 taatgcgtag gcggccagga ctttgtgtgg tgcgaagcga ctgatgagcc aggtaccggt
    13201 gaaacgacca ataaagaagc acaccatggt tccggttaaa tagtttgcgg caaagcctgc
    13261 agtcatacct ggaatttctt ctacagcgta gcgaatcaaa tagctccagc aggccgtttg
    13321 cgcgccgacg tagcagaatt gcgctaatac cgcccagcgc cagtgacgaa tacgcgccag
    13381 gcgagaaagc gatgcggaga acgatccttg tttggcgtca ctgtgattat cactctgcaa
    13441 tgccgggaat ttcgtcagca tgatcagcag ggcgaccagt aacactatag ccacgatgat
    13501 catataaggt gtctgtaccg ataataccag gctgtgttta tacgcactca attgctctgg
    13561 agacatttta tcgagaacgt cttgcgattg atgtggcacg ttagacaaaa taagactttg
    13621 cccaaagaca accgcgataa ttgcgccaaa tgagttaaat gtttgcgcaa gatttaagcg
    13681 gaagtgacca ctgctttccg gccctaatac cgtaacaaaa gggtttgcgg cagtttccag
    13741 acaacctaat ccggctgcaa taataaatag gccaactaaa aataaggtgt agttcattat
    13801 ttctgcagcg ggccagaata atgcagcgcc caaggcatat aaaaataatc cggtaataat
    13861 tcctgcttta taactgagtt ttttcatcaa tatcccagca gggattggga taatgaaata
    13921 accaaagtaa aaggccgatt ggatcaggcc agcctggaaa tttgtcagcg taaaagcctg
    13981 ctggaattga ggtaataata tgtcgttaag attattggct accgcccaaa gaaaaaacag
    14041 tgagcacagc agcgcgaatg gaataatgta acttctgctt tgccctgcat ctttatctac
    14101 cgcacggtaa ctctgcgttt gtattgatgt gtttcccata gcatcctctt aggttcagaa
    14161 gcttaagcac gtaatcggtt atgttttcaa aagtgcaaaa ccagcgagtt cgggcttcat
    14221 atcggtgaat atttgcctgt ctgaaatgaa ctcatggatt cattttcaaa taaaaaacgc
    14281 tctgtaatat gacggcggtc acacttatta gctggaaggt gactttatgt gactaccatc
    14341 actttaataa tcgaaatttc atgcccgttt cagaaaataa atcataaaaa acacaaacca
    14401 gatgaccgta ttaattgttt atgccgaaaa cgggcattcc tggcgagggg aaatggaaaa
    14461 atgaccgttt tgataaatat ttttaagagc ggtcattttt aacggggtta taaaaagaga
    14521 acttgcagtt atttactgtg attagttgag ccaggtcaca aaatataagc ttgtgaattt
    14581 ctttcttgtt aatcaggaag aagatggtgg gtaattaaac ggctaattca atagtgtgaa
    14641 aggaacaaca ttattgccct gttttgaatc agagagaggt agctaatgga acgaaataaa
    14701 cttgctcgtc agattattga cacttgcctg gaaatgaccc gcctgggact gaaccagggg
    14761 acagcgggga acgtcagtgt acgttatcag gatgggatgc tgattacgcc tacaggcatt
    14821 ccatatgaaa aactgacgga gtcgcatatt gtctttattg atggcaacgg taaacatgag
    14881 gaaggaaagc tcccctcaag cgaatggcgt ttccatatgg cggcctatca aagcagaccg
    14941 gatgccaatg cggttgttca caatcatgcc gttcattgca cggcagtttc cattcttaac
    15001 cgaccaatcc ccgctattca ctacatgatt gcggcggcag gcggtaattc tattccttgc
    15061 gcgccttatg cgacctttgg aacacgcgaa ctttctgaac atgttgcgct ggctctcaaa
    15121 aatcgtaagg caactttgtt acaacatcat gggcttatcg cttgtgaggt gaatctggaa
    15181 aaagcgttat ggctggcgca tgaagttgaa gtgctggcgc aactttacct gacgaccctg
    15241 gcgattacgg acccggtgcc agtgctgagc gatgaagaga ttgccgtagt gctggagaaa
    15301 ttcaaaacct atgggttacg gattgaagag taatttcgta aagcaacaag gagaaggatg
    15361 atggctaaca gaatgattct gaacgaaacg gcatggtttg gtcggggtgc tgttggggct
    15421 ttaaccgatg aggtgaaacg ccgtggttat cagaaggcgc tgatcgtcac cgataaaacg
    15481 ctggtgcaat gcggcgtggt ggcgaaagtg accgataaga tggatgctgc agggctggca
    15541 tgggcgattt acgacggcgt agtgcccaac ccaacaatta ctgtcgtcaa agaagggctc
    15601 ggtgtattcc agaatagcgg cgcggattac ctgatcgcta ttggtggtgg ttctccacag
    15661 gatacttgta aagcgattgg cattatcagc aacaacccgg agtttgccga tgtgcgtagc
    15721 ctggaagggc tttccccgac caataaaccc agtgtaccga ttctggcaat ccccaccaca
    15781 gcaggcactg cggcagaagt gaccattaac tacgtgatca ctgacgaaga aaaacggcgc
    15841 aagtttgttt gcgttgatcc gcatgatatc ccgcaggtgg cgtttattga cgctgacatg
    15901 atggatggta tgcctccagc gctgaaagct gcgacgggtg tcgatgcgct cactcatgcg
    15961 attgaggggt atattacccg tggcgcgtgg gcgctaactg atgcgctgca cattaaagcg
    16021 attgaaatca ttgctggggc gctgcgagga tcggttgctg gtgataagga tgccggagaa
    16081 gaaatggcgc tcgggcagta tgttgcgggt atgggcttct cgaatgttgg gttagggttg
    16141 gtgcatggta tggcgcatcc actgggcgcg ttttacaaca ctccacacgg tgttgcgaac
    16201 gccatcctgt taccgcatgt tatgcgttat aacgctgact ttaccggtga gaagtaccgc
    16261 gatatcgcac gcgttatggg cgtgaaagtg gaaggtatga gcctggaaga ggcgcgtaat
    16321 gctgctgttg aagcggtgtt tgctctcaac cgtgatgtcg gtattccgcc acatttgcgt
    16381 gatgttggtg tacgtaagga agatattccg acactggcgc aggcggcact ggatgatgtt
    16441 tgtaccggtg gcaacccgcg tgaagcaacg cttgaggata ttgtagagct ttaccatacc
    16501 gcctggtaaa tgcgctgatg tgataatgcc ggatacgacg tttgcggtgt atccggcacg
    16561 ccgttaccgt accaaccgca attgctgtaa attgccgtcg atatgcaaat cggtttgtaa
    16621 gcgggcaata tcgcggcaca gaaacgccat ctctttatgg gtttctaatt ttttgcgcca
    16681 cttttcggca accgcatcca gattcccata tatcccttcc agactctgaa actcgaccag
    16741 cagctgcgtg gcgctttttg gtccgattcc cgcaacaccc ggtacctttg aactgctgat
    16801 ccccgcaagt ccccagtaat cgggcaactg ctgcggttga acgccaaatt ctttatcgat
    16861 aaatggcgca tccagccaac gtttctggaa gtaatcacga atacgtaatg tcggtgaaag
    16921 taactgacag tagcctttat ctgtcgaaac aatcgttgcc tgatgcccgg cctgtgtcac
    16981 tttgaccgcc agcgtggcgg ctaagtcatc ggcttcgttg ccgctggctg accaacacgg
    17041 gacgccgcgt tgctcaaagg cggcgcgtaa tgcaggcatc tcgtcgtgca actcttccgg
    17101 cattggcggt cgacccgctt tgtaatctgg taaacgctga tgacgccagc cgctactgcg
    17161 gttttcatca tcaaaaacgg cgaccgcgtg ggttggctgg ctgtgcataa tgagctgatc
    17221 gagcgcgtgc tggcaggttt cgacacaggg cgaaccctga acggcatgaa tgcgacgaat
    17281 aagattcagt gcatcgacaa taagtaaatg aacagccacg ataaccccct taaagatcag
    17341 gcgataaggg taacattcga tgccggaaca ggctatggct ggttgagaaa acaggaagag
    17401 gcctcgcaaa acgaggcctc tggagagcga ttaatcgcag gcaacgatct tcattgccag
    17461 gccgccgcga gaggtttcgc ggtacttggc gttcatatct ttacctgttt cgtacatggt
    17521 ctcgataact ttatcgaggc agacgcgcgg ctcgctggta cggcgcagcg ccatacgcgc
    17581 ggcgttcacc gcttttactg ccgcaatggc gttacgctcg atgcatggca cctgcacctg
    17641 tccagcaacc gggtcacacg tcagaccgag gttatgctcc atggcgattt ccgccgcgat
    17701 gcacacctgc gccgggcttg cgcctaacag ttctgccaga ccagctgccg ccatagagca
    17761 cgccacgcca acttcaccct ggcaacccac ttcggcacca gaaatcgacg cgttcatctt
    17821 ataaagagaa ccaatggcgc tggctaccag caggtaacga gccagtgagt tagcgttcac
    17881 ttcgcggata aacttatcgt agtacgccag cacagccggg ataatcccgc acgcaccgtt
    17941 agtcggcgca gtcaccacgc gaccgccagc agcgttctct tcgttcactg ccagtgcaaa
    18001 catgttgatc cagtcaacaa ccgccatcgg gtcagtggtg gttttatcct ggctgaccag
    18061 catccggcgt agtgccgcag cacggcgtgg aacgcgcagt ttgccaggca acacgccttc
    18121 ggtggaaata ccgcgctcaa taccgccacg catgacttcc cagacgttcg ccaggtgctg
    18181 ttccagctct tctttgctgt gcagcgccag ctcgtttttc atcatcaggc cagagagtga
    18241 cagcccggtt tcctgacaat gtttttgcag atcggctgct gaactgtacg gataaggaac
    18301 ttcaaccggt gcgctattct gctggccaaa atgctcttca tcaacgataa agccaccgcc
    18361 aatagagtag taagtctggc tgtaaacgac tttatcgccc gccagcgcgg taatgcgcat
    18421 accgttttca tgcagagaaa ggttgtctgc atggaagttc atgcactgat caaccgggaa
    18481 ctccacttca tgctgaccgt ttgccagcat caggcgacca tgagtattca catcctgaat
    18541 aaaaccaggg atggaatcga tatccacggt atccggcagg ttacccgcca ggcccataat
    18601 aatggcgata tcagtgtggt ggcctttacc ggtcagagag agcgagccgt acacgtcaac
    18661 caccacgcgg gtcacgtctt taagcagatt acgggcaatc agatcgtcgg taaattgttt
    18721 acccgctttc attggtccaa cggtatgaga actggaaggg ccaatgccga ttttgaaaat
    18781 atcgaatacg ctaatcatag gaaatacatc gcgttaaaac ggaggaagcg ccgcccgaaa
    18841 gcggcgcgaa aggacttagc tgaacagaga gtagaagatt gcggagattg caatcagacc
    18901 catcacgaca acgaatacgt tgctgatgtg accgctgtac ttacgcattg ccggtacttt
    18961 ctgaattgcg tacatcggca tcaggaacag gatcatcgcg atgattgggc cgcccagggt
    19021 ttcaatcata cccaggatgc tcgggttcag ggtggcaaca atccaggtcg ttaccagcat
    19081 gaacagcgca gtgatacggt tcagcttgtt gatttcgata gacttacctt taccacgcag
    19141 agatttaatc accataccgt tgaagccttc acgtgcgccc aggtagtggc cgaggaagga
    19201 tttggtgata gcgataatcg cgataatcgg agccatccac gcgataaccg gtgcgttaaa
    19261 gtggttagcc aggtaagaca gaatcgagat gttctgctct ttagccgcag ccaggtctgc
    19321 cggagtcagg ctcagtacgc agctgaagac gaagaacatt acggtcagca ccatcatgat
    19381 gtgtgcgaat gccaggatct tcgagcattt ctgttctgcc atatcgccgt actcttcacg
    19441 cttcgcaacg gcgaaagaag agatgatcgg agagtggttg aacgagaaca ccattaccgg
    19501 aattgccagc cacagggtca tccacagacc gtttccggtt gcagatgcag tgtccagaga
    19561 cagcgtttcc agtgctgcgc cgttccactg cgggatcagg tacagagcca gcagcatcag
    19621 tacgccaaca aacgggaata ccagaatact catcgcttta acgatcatct gctcaccaaa
    19681 gcgaacgatg gtcatcatac ccacgatcag gatcagcgac agaatcgcac gcggcggtgg
    19741 cgtcataccc agctggtgag acatgaagct ttcaactgta ttggtgattg ccacgctata
    19801 aaccagcagg atcgggtaga tagcgaagaa gtagagcagg gtaatcagtt tacctgcgcc
    19861 aataccaaag tgttcttcta caacctcggt gatgtcttcg cccgggtttt taccagacag
    19921 tacgaagcga gtcaggccgc ggtgagcaaa aaacgtcatc gggaacgcaa ggatagccat
    19981 gatgatcagc gggatcatac cgccaacacc ggcgttgatt ggcaggaaca gcacgcccgc
    20041 gccgattgcc gtgccgtaaa ggcccagcat ccacatggtg tctgtcttgc gccaggcact
    20101 acgagagtct ttcgacgcaa tcgtgctggt ttgagtcgtt tccatctatt tctcctggag
    20161 gaatgcaaat aattcagctt ttagccagtt cagcccggga aggtgcctga ccgtaaaaat
    20221 gaaatctgtc tatcggcgat ggcttaatca tttaccgccg taataatttg agggcggaaa
    20281 gatacacaca agttatgtat ctatcagtga tcttgatctc aaatacaata atctacttgg
    20341 aagatagatt atcccaacgc tttactctgt aaatcaaaag taaaagagaa tttaagggca
    20401 atatttgccc attaaacgac atctgacttc ttccggggcg cgcaatatat gtaggttgaa
    20461 ctgaaaaaat caatactttt agttaatcat ggcgataatt tctttttcat agcgataaaa
    20521 tacggtttat gaaaataggg aaacgtttgc attgcaacat aaattgtatg acatattaac
    20581 tgtgatgata tttgtcgcgt gagggtggta agaaacattc atataacaaa tgaaatgagt
    20641 tgatgagtaa gtgaccaaaa acgagaaaac ccggcaacaa acgtcaccgg gggaggggag
    20701 attacgtgca gatttcgtag caagggatgt aggctgagcc tggcaacttc atacgatgct
    20761 gggcaacaaa accctgtagc aggtcgtcca tacgacgcat aatctcttta tcgccgttga
    20821 ttttgtaagg accaaactct tcaatggcgc gaataccgac ttcttttacg ttacccgcca
    20881 caataccgga gaacgcacgg cgcaggtcgg cagccagcac ttcaacaggt tgatccgggt
    20941 aaagcttcag attagccata ttctcgtgag acggctcaaa cggcatttgc aaatctggcg
    21001 caatgcgcat tgaccagtta aagctgtagg catcgcctgt atcacggcga ttttctttca
    21061 ccagcggcat cgattttttc atctgacgag cgacttcagc ggcgtcatca atgatgatgc
    21121 ggtaatggcg gcgcgcgttt tcgcccagcg tatgtacgac aaactcgtcc agtacgcgga
    21181 agtagtcggc gctctctttc gggccggtga ggatcaatgg taaaacctga tctttgttgg
    21241 ccgggttcat taaaattccc agcaaataca gcaactcttc tgccgtaccc acacctccag
    21301 ggaagataat gatgccgtga gcgatacgga caaacgcttc cagacgtttt tcgatatccg
    21361 gcatgatgat caattcgttg accagcgggt taggcggttc agcggcgata atcgacggct
    21421 ctgtcatacc aataaaacga ctgtctttgt aacgctgctg cgcgtgtccg accgcagcac
    21481 ctttcatcgg cgcttccatc gctcccggac cacagccggt gcagatattc agctcacgca
    21541 ggcccagctg gtttccgacg cgacgggcat acaaatactc gttttcgtta attgagtgac
    21601 cgccccagca gaccaccata tttggcgctt cacccacatg cagcgcgcga gcgttacgca
    21661 agatggaaaa gaccaggtta gtgatgtgga cggagttgtc gagattcagg ttgggaaaac
    21721 gaacggtatt atggatttgc ccgtaaacga agagaatgtc tcgcagaacc gcgaacaagt
    21781 tggcctgcaa agcgcgaata attcgcccat cgacaaaagc ctcttccggg ggattaatca
    21841 gttccagctt tacgccgcgt tcacggcgca ggacgttaat atcgaaattt tcaaaacgag
    21901 acagcaattc tttgctgtta tcggtcaaac taccggagtt cagtacggca agtgaacagt
    21961 tgcgaaacag ttgatagagg tcgctgctgg cggtgcgttt aagcatatcc acttccagct
    22021 gcgacaacat atccatggag ccaagcgggc taatatgtgt aatcaagaaa aactccttat
    22081 gggacgaata tcgtcattcc ctttgattac aatagccctg cctgatgacc tttcacaacc
    22141 ttctgcgtga atccagcacg caaaattgag aaaaaattta ttgccgaacc agtcttgtgg
    22201 tcgatgggac aaaatcgcta ttactgcgcc acgggttaat gtccagaccg ccacgacggg
    22261 tataacgtgc gtaaacgctt aatttctctg gctggcagaa gcgtaacagg tcattaaaga
    22321 tgcgttccac gcactgttcg tggaactcgt tgtgatgacg gaatgagacc aggtaacgca
    22381 gcagtttttc tctgtcaatt tggcgtccac gatactgaat ttggatcgaa ccccaatctg
    22441 gttgatgggt gatcaggcag tttgatttca gcaggtggct gacaagcgtc tcttccacta
    22501 ctttttcacc gctggtggca ttctccagat agtcagtagt gaattcatag ttatcgatag
    22561 tgatatcctg gtcatcaatg caggtgccat tgaaatgacc tatcggctgg ccttccagtt
    22621 catcaagacg atataacgcc acgctaacct taccctgggc gcaagtgctt aagtcgcgct
    22681 ccagcgtctg gcgcacctca tcccagttat taaaacgcgt ctggttaaaa ctgttgagat
    22741 agagcttaaa actcttcgac tcaatcagat ttacgctggt gtaatcaagt tcaacatgac
    22801 cgacagcgac ctgcggcaaa cctttcgcat tcagccagga aagttcatac agcgtccaga
    22861 tatccgtacc gtgaaaaggc aggttatccg ctttcagacc cagcgggtcg cgattcaggc
    22921 tgcgtggaac gccttgcagt aggctggcgt cataggtatc ccggtaatcg gttgattttc
    22981 caagagtcag gcccgcaagt gcctgatggt ttgcataaga agacatgttt caccgtcata
    23041 aaccagataa actaaacgac aagtgtatct ggtttaagtg aagagagaaa ttagtggacg
    23101 atttgaccgc acaagccctg aaagatttta ctgcacgcta ctgcgatgcg tggcatgaag
    23161 agcataaaag ctggccgtta agtgaggaac tgtacggtgt tccttcaccg tgcattattt
    23221 caactaccga agatgccgta tactggcaac cccagccgtt taccggggaa caaaatgtaa
    23281 acgcggttga acgcgctttt gatattgtga tacaacccac aattcatacc ttttatacga
    23341 ctcagtttgc cggggatatg cacgcgcagt ttggcgatat caaactgaca ttgctgcaaa
    23401 cctggagtga agacgacttc cgtcgggtgc aggaaaatct gattggtcat ctggtgactc
    23461 agaaaaggct aaaactgccg cccacgctat ttatcgcgac gctggaagaa gagttagagg
    23521 tcatctcggt gtgtaatctc tccggtgaag tgtgcaaaga gacgctggga acacgtaaac
    23581 ggacgcattt agcctcaaat cttgcggaat tccttaatca acttaagcct cttctgtaat
    23641 ccaaataccc catctggttg tgagagatct cttacagact ctgtaggaga tcgccaggaa
    23701 ataagcgaat acttaaaaag ataagaatcg ttattttcat ttaaaatcaa aatgttgatt
    23761 gttaattctt aactttcata tgaaattttc cttaaggcat tgtctgtaag cgccttgtaa
    23821 gacttcgcga aaaagacgat tctatcttcg tcgacaggga gtcagacaac gaagtgaaca
    23881 tcaggatgat gacacttctg caggacacac caggatggtg tttcagggaa aggcttctgg
    23941 atgaagcgaa gaggatgacg caggacgcgt taaaggacac ctccaggatg gagaatgaga
    24001 accggtcagg atgattcggt gggtcaggaa ggccagggac acttcaggat gaagtatcac
    24061 atcggggtgg tgtgagcagg aagcaatagt tcaggatgaa cgattggccg caaggccaga
    24121 ggaaaagttg tcaaggatga gcagggagca acaaaagtag ctggaatgct gcgaaacgaa
    24181 ccgggagcgc tgtgaataca gtgctccctt tttttattcc tgctatcctt cgcggcagtt
    24241 tttcttattg aggttgcttt atgaccactc atgaccgcgt tcgtctccag ttgcaggcgc
    24301 ttgaagcgtt actgcgtgaa catcagcact ggcgaaacga tgaaccccag ccgcatcaat
    24361 ttaatagtac ccaaccgttc tttatggaca ccatggaacc actggagtgg ttgcagtggg
    24421 tgctgatccc gcgaatgcac gatttgttag ataacaaaca gccgttaccg ggcgcttttg
    24481 ccgttgcacc ctattatgaa atggcgctgg cgacggacca tccgcaacgc gcgcttattc
    24541 tggctgagct ggaaaaactg gatgctcttt ttgcggatga tgcgagctaa tgctggaaat
    24601 actctatcag gatgaatggc tggttgcggt aaataaaccc tccggctggc tggttcaccg
    24661 cagctggctg gatcgcgacg agaaagtagt ggtcatgcaa accgtgcgtg atcagattgg
    24721 ccagcacgtt tttaccgctc atcgtctgga ccgacccact tctggtgtgt tgttgatggg
    24781 attatccagc gaggccggac ggctgctggc acaacagttt gaacagcacc aaatccagaa
    24841 acgttaccat gcgattgtgc gcggctggtt gatggaagaa gcagttctgg attatccact
    24901 ggtggaagaa ctggacaaaa tcgctgataa atttgcccgc gaagataaag gctcgcagcc
    24961 agcagtgacg cattatcgcg gtctggcgac cgtagaaatg ccggtagcga ccggacgtta
    25021 cccgaccacg cgctacggcc tggtggaact ggagccgaaa accggacgca aacatcagct
    25081 tcgccgacat cttgcccatt tgcgtcatcc gattattggc gatagcaaac atggcgattt
    25141 acgccagaat cgcagcggtg ctgagcattt tggcctccag cggttaatgc tacatgccag
    25201 tcagttgtca ttgacgcatc cttttactgg cgagccgctg actattcacg cgggcctgga
    25261 cgacacctgg atgcaggcgt tatcacaatt tggctggcgc ggtctgcttc ctgaaaatga
    25321 aagggttgag ttcagtgcgc catcgggtca ggatggagag ataagttcct aatccaggga
    25381 gaaaacggta tggcggaaat tggtattttt gtcggcacca tgtacggaaa ttcgctgtta
    25441 gtggcggaag aagcagaagc aattctgacc gcacagggcc acaaagcaac ggtatttgaa
    25501 gatcctgaat taagcgactg gctgccttat caggataagt atgttctggt ggttacgtcc
    25561 acgaccgggc agggcgacct tcctgatagc attgtgccgc tctttcaggg aatcaaagat
    25621 agtctgggct tccagccgaa tctgcgttat ggcgtgattg cgctcggcga cagcagttat
    25681 gtaaatttct gcaatggcgg caaacagttc gatgccttgt tgcaggaaca gagcgctcag
    25741 cgggttggcg aaatactgtt gattgacgcc agtgagaacc cggaaccgga aacggaatca
    25801 aatccgtggg tcgaacagtg gggcacgctg ttgtcctgat atgttcagcg agcggtaaat
    25861 gtcgttttag cggtgctgaa tcgaatcttt tcaggcaaat gccagtaaaa actgcttcat
    25921 agcgcggatt tttactggcg tttgcctgga gtcaagcgat ccatttcata ctcttcttta
    25981 tttcttcgtt ttaacccttc ctttcttgtt cttgttttca tttccgtgaa gtggattcca
    26041 ccgtccaggg ctaatgccaa aatcgggcct cattgaacgc attaatgttg tgttgttgca
    26101 cggtgagccg ctatggcgcg ctttttatac tgctattgcc agatataaac acgcgccgta
    26161 ttcggcgaac gacctataaa aacggcaaaa aacaccctac gtcacctctg atttcctggc
    26221 tatgtcgcag tccagagtga gcgtggctaa cgcgaatttt caggagtgca acaatgagtt
    26281 ctttaagtca ggctgcgagc agtgtggaaa aacgcacgaa tgctcgttac tggatagtgg
    26341 tgatgttgtt tatcgtcaca tccttcaact acggcgaccg cgctacgctt tctattgccg
    26401 gttcggaaat ggccaaagat atcggccttg atcccgtggg aatgggctat gtgttctctg
    26461 ccttctcatg ggcttatgtt atcgggcaga tccctggtgg ctggttgctg gaccgttttg
    26521 gttcaaaacg cgtctacttc tggtcgatct ttatctggtc gatgtttacc ttgctgcaag
    26581 gcttcgtcga tatctttagt ggattcggca ttatcgttgc cctgtttact ctgcgcttcc
    26641 tggtcgggct tgctgaagcg ccatctttcc ccggcaacag tcgcattgtt gcggcctggt
    26701 ttccggcgca ggaaagggga acggcggtgt cgatttttaa ctccgctcaa tacttcgcaa
    26761 cggtgatctt cgcgccgatt atgggctggc tgacgcatga agtgggctgg tcacacgtct
    26821 tcttctttat gggcggtctg gggattgtca tcagctttat ctggttgaaa gtcatccacg
    26881 agccaaatca acatccgggg gtaaatcaga aagagctgga gtacatcgcc gcgggtggcg
    26941 cgctgatcaa tatggatcag caaaacacca aagttaaagt gccgttcagc gtgaagtggg
    27001 ggcagatcaa acaactgctc gggtcacgga tgatgatcgg cgtttatatc ggtcagtact
    27061 gtatcaacgc cctgacttac tttttcatta cctggtttcc ggtttatctg gtgcaggcgc
    27121 gtgggatgtc gattctgaaa gcgggctttg tggcttccgt tccggcggtt tgcggtttta
    27181 tcggcggtgt gctgggtggg attatttccg actggctgat gcgccgcacg ggatcgctga
    27241 acattgcgcg taaaacaccg atcgtaatgg gcatgttgct gtcgatggtg atggtgttct
    27301 gcaactacgt caacgttgag tggatgatca tcggctttat ggcgctggcc ttcttcggta
    27361 agggcatcgg ggcgctgggt tgggcagtaa tggcagatac cgcgccaaaa gagatcagcg
    27421 gtctttccgg tggcctgttc aacatgttcg gtaacatttc tggcatcgtc acgccaatcg
    27481 caattggtta tatcgttggc acgactggct cctttaatgg ggcgctgatt tatgttggtg
    27541 ttcatgcctt aatcgcggta ctgagctacc tggtgctggt gggcgatatc aagcgtatcg
    27601 agctgaaacc tgtcgcgggg caataagatg acgacacaat ccagtcctgt tattactgat
    27661 atgaaagtca ttccggtcgc cgggcacgat agcatgttgc ttaatattgg tggggcacat
    27721 aacgcatatt tcacccgcaa tattgtggta ctcaccgata acgccgggca taccggcatt
    27781 ggtgaagcgc cgggcggaga ggtgatttat caaacactgg tcgatgctat tccgatggtt
    27841 ctgggccagg aagttgcgcg cctgaataaa gtggttcagc aggtgcataa aggtaatcag
    27901 gcagccgatt ttgatacctt cggcaaaggt gcctggactt ttgaattgcg cgttaatgcc
    27961 gtggcggcgc tggaagccgc cttgcttgac ctgctaggta aggcgctgaa tgttccggtc
    28021 tgcgaactgt tggggccagg caagcaacgc gagactatta ccgtcctcgg ttatctgttt
    28081 tatatcggtg atcggaccaa aaccgatctt ccttatctgg aaaatacgcc gggcaaccat
    28141 gagtggtatc agttgcgcca tcagaaagcg atgaacagcg aagccgttgt gcgtctggcg
    28201 gaagcctcac aggatcgcta tggctttaaa gatttcaaac ttaagggcgg agtgttacct
    28261 ggcgagcaag aaatcgacac tgttcgtgca ttgaagaaac gcttcccgga tgcgcggatt
    28321 accgttgatc ccaacggtgc atggctgctt gatgaagcca tttctttgtg caaagggctg
    28381 aatgatgttc ttacctatgc cgaagatccg tgcggcgctg agcagggttt ctccggtcgt
    28441 gaagtgatgg cggagtttcg acgggcgacc ggcttgcccg tcgcgactaa catgatcgcc
    28501 accaactggc gcgaaatggg ccatgcggtg atgctcaatg cggtagatat tccacttgcc
    28561 gatccgcact tctggacgct ttccggtgca gtccgcgtgg cacagctttg cgacgactgg
    28621 gggctaacct ggggctgcca ttctaataac catttcgata tttctctggc gatgtttacc
    28681 catgtgggcg cggcggcacc gggtaatcct accgctatcg atacccactg gatttggcag
    28741 gagggcgatt gtcgcctgac caaaaatccg ctggagatta aaaacggaaa aattgccgtt
    28801 cctgatgcgc ccggtctggg cgtggaactg gactgggaac aggtacaaaa ggcacatgag
    28861 gcctataaac gtctgcctgg cggtgcgcgt aacgacgcag gtccgatgca gtacctgatc
    28921 cccggctgga cctttgaccg taaacgtccc gttttcggcc gtcactgatt ctgaataagg
    28981 acataaatat gagttctcaa tttacgacgc ctgttgttac tgaaatgcag gtcatcccgg
    29041 tggcgggtca tgacagtatg ctgatgaatc tgagtggtgc acacgcaccg ttcttcacgc
    29101 gtaatattgt gattatcaaa gataattccg gtcacaccgg cgtaggcgaa attcccggcg
    29161 gcgagaaaat ccgtaaaacg ctggaagatg cgattccgct ggtggtgggt agaacgctgg
    29221 gtgaatacaa aaacgttctg acgctggtgc gtaatacatt tgccgatcgt gatgccggcg
    29281 ggcgcggttt gcagacattt gatttgcgta ccactattca tgtagttacc gggatagaag
    29341 cggcaatgct ggatctgctg gggcagcatc tgggggtaaa cgtggcatcg ctgctgggcg
    29401 atggtcaaca gcgtagcgaa gtcgaaatgc tcggttatct gttcttcgtc ggtgatcgca
    29461 aagccacacc gctgccgtat caaagccagc cggatgactc atgcgactgg tatcgcctgc
    29521 gtcatgaaga agcgatgacg ccggatgcgg tggtgcgcct ggcggaagcg gcgtatgaaa
    29581 aatatggctt caacgatttc aaactgaaag gcggtgtact ggccggggaa gaagaggccg
    29641 agtctattgt ggcactggcg caacgcttcc cgcaggcgcg tattacgctc gatcctaacg
    29701 gtgcctggtc gctgaacgaa gcgattaaaa ttggtaaata cctgaaaggg tcgctggctt
    29761 atgcagaaga tccgtgtggt gcggagcaag gtttctccgg gcgtgaagtg atggcagagt
    29821 tccgtcgcgc aactggcctg ccgaccgcaa ccaatatgat cgccaccgac tggcggcaga
    29881 tgggccatac gctctccctg caatccgttg atatcccgct ggcggatccg catttctgga
    29941 cgatgcaagg ttcggtacgt gtggcgcaaa tgtgccatga atttggcctg acctggggtt
    30001 cacactcaaa caaccacttc gatatttccc tggcgatgtt tacccatgtt gccgccgctg
    30061 caccgggtaa aattactgct attgatacgc actggatctg gcaggaaggt aatcagcgcc
    30121 tgaccaaaga accgtttgag atcaaaggcg gtctggttca ggtgccggaa aaaccggggc
    30181 tgggtgtaga aatcgatatg gatcaagtga tgaaagccca tgagctgtat cagaaacacg
    30241 ggcttggcgc gcgtgacgat gcgatgggaa tgcagtatct gattcctggc tggacgttcg
    30301 ataacaagcg cccgtgcatg gtgcgttaag acagaaaacc aaaaccggct ctatctggag
    30361 ccggcttgag actgatgcta aacgcaaaat agcctgttgt gctatgtatc aggtctacat
    30421 cattttttaa ttcattgaat ttaattggtt ttgtaggccg gataaggcgt taacgccgca
    30481 tccggcataa acacaggcac tttgtcacca atctgaaacc agcgtcataa aaagccgatt
    30541 gctactcgac aagacgtccg ttacccgaga attttgctgg cttcgcgcgc tacattatcc
    30601 atctcgtcca acagttccag cagctccggc tccaaatctt cttctttagt accgctacgt
    30661 agctgttgtt cgatcagttg gcagagattc ttcatacgcg gcacaccgct atagccgcaa
    30721 ctgccatgca gtttatgaat caaatcaacc aggccttccg ggttttctcc aaccagctgt
    30781 tcctcaactt tgttgcgaac ttcaggcagg aaatcgagta acatttgcag catatcgcgc
    30841 gctaaatcgg tttttcctgc tgcctggcgt agtgccagtt gccagtcgag ggtcgcattc
    30901 gggttcacca caatttcgtt gacttcgggc gtcacgacgc gagaggaaat accgctgcca
    30961 ggcttgtagc gcaacaacaa attatgcaat cgctcttctt caatcggttt cgccagataa
    31021 tcgctcatcc ctgcgccaag cagcttctct ttttgcccgg ccattgcatg cgccgttacc
    31081 gcgataaccg gcgtttgttg ctgatggggg agctggtgga tgagctcgca ggcccgaatg
    31141 ccatccatgt caggcatttg aatatccatt aagatcaaat cgaacggcat ctgtttcgcc
    31201 cgttcaaccg cctgatgccc gctatcgcaa agttccacat gttgcaccat atcttccagc
    31261 aatgcgccga taagtttcag gttagcgggg ttgtcatcaa ctgccatgac tgtcattgcc
    31321 agcttgcttt catcggttac aggcaaaagc gtgttttgtt tgtgatgaca aaattccgtc
    31381 agggcaggca acaggcgcgt aggtgttaat ggtttcagca gacacgcgcc gataccatct
    31441 tgcttgagtt tttctgcatt gacttgtgca tggcaaggaa gtgccagcat caggaaatcg
    31501 gtcatcgata ccgctttcgc taatcgctca tgttgcattg ttagcggctc gcggaaggtc
    31561 accgcgatgc ctaacaacat catgtcgtaa tgcgcgggag gcagcgcgga gaacgttggg
    31621 ctataaacca cttccagcgg cgtttcactt aaaatatcca gcgtgcattg cgctgctgcg
    31681 gagtttggtt cgacataggc caggcgctta ccggcgaggc actgggtgga tggcccttcg
    31741 ataataatgt tcgggttcag atcgagatta atgtggaacc agaaagttga accgcgattc
    31801 ggctggctat ggaacgaaat atcgccgccc atttcattaa ccagtttttg tgtaatcacc
    31861 agccccagac cggtgccacc atgacggcgg gaaatactgg catcagcctg tcggaaggcc
    31921 tggaataagc gcgattgatc gcgttcagga ataccaatgc cggtatcccg aatctgcact
    31981 tcaatctgca ctttggtatt actcagcgca cgtttttcta ccagaatatc aatgttgcca
    32041 ttctcggtga atttaattgc attccccacc aggttagtga tgatttgctg taatcgcagt
    32101 gggtcgccga tcacgttatc aggcacgtcg cttttaatat tgagcgtcag ttccaaccct
    32161 ttatcgtgag aagaatgtgc cagcagagta acgacttcat ccagcgtgct gcgtagtggg
    32221 aatggaatac tttccagaat cagcttacct gcttccagtt tcgagaagtc gagaacatca
    32281 ttaataattg ccagcaaatt atttgccgaa cgttcaatcg tattcaggtg atcgcgctgc
    32341 gttggtgtta attctgtttt cagcgtcagg cgggtaaagc caataacacc attcagtggt
    32401 gtacgcagct cgtgtgacat atttgccaga aactcggatt taatacgcgc cgcttcctgg
    32461 gcgcgctttt tcgccagatc taactcaacg ttctgaattt ccatctgctc cagcgtttca
    32521 cgcagatcgg acgtcgcctg gtcgatattg tgctgcatct cttcgtgata agcagccagc
    32581 gacattgcca tcgagttgat accgtttttc agcatatcca gctcgccgag cataaatcct
    32641 tccacccggc tgtcgagttg cccgcgacgg atgcggtcga cggtattcac catgttgcga
    32701 atcggaccgg ttacatcgcg cattaagcgc cagccaaaaa taagcgcaat accgatacaa
    32761 aacagcatca tcacgctgga aataaagatc tctttatatt gctgcaagcg aaccgattta
    32821 agatccagct caagcgcaat atatcccaac atattttgac tatttttggc atcgctactg
    32881 ggcgattcgt cgggggagta actctcagaa ataatcggcg tgcggaggat cataatatcg
    32941 ccgtcacgag tgacagtgag ctggcgagga aacggcacgt tgctgccgag ctgcattgat
    33001 gagggatcaa gatgaaaatt ggaggtgaca aagagtcggt tattttcatc ataaaccgaa
    33061 atcgcgcgaa caatatcgga atggcgacga tgcagtacgc ttattaactg accgatagat
    33121 tcgcgatttt gcaggctcat gccatattca gtagaaactg caagcggctc aataatgctg
    33181 gcaccggcat cttccagttg acgctgcaag tcgttatagc gatgcacgac gaaaaagata
    33241 ctcagcaata aaccaataag gacggtcggt gccaggatca gaatcatcat gcgtgcgcgc
    33301 aggctgtagt tggtcatgga gttccgttat gggacaatta aggtcacact gttaaattga
    33361 gaaagtctcg ccaatggcgc aattctactc tgcaaaacga cgcacgacga cgcgtcagat
    33421 cataaccgtt tcagtcaacg acctcgactc ttttggtcag ggcgtggcgc gacataacgg
    33481 caaagcgtta tttattcctg gattactgcc acaggaaaac gcggaagtta ctgttactga
    33541 agataaaaaa cagtacgccc gcgctaaagt cgtgcgccgg ttaagcgata gcccggaacg
    33601 cgaaacgcca cgctgccctc attttggcgt atgcggtggc tgtcagcaac aacacgccag
    33661 cgtggattta cagcagcgaa gcaaaagtgc ggcactcgcc cgattaatga aacacgaagt
    33721 ctctgaagtg atcgccgatg ttccctgggg ctatcgccgt cgcgcgcgtt taagtttgaa
    33781 ctacttaccg aaaacacagc aacttcagat gggctttcgc aaagcgggct ccagtgacat
    33841 tgtcgacgtc aaacaatgcc ccattttagt gccccaactt gaagcattgc tgcccaaagt
    33901 cagggcatgc ctgggcagct tacaagctat acgccatctt ggtcatgttg aactggtaca
    33961 ggcaaccagc ggcacgctga tgattttgcg ccataccgca ccgctaagtt cggctgatcg
    34021 cgaaaaactg gaacgctttt cgcattctga aggcctggat ctgtatctcg cccccgatag
    34081 tgagatactc gaaaccgtct ctggtgagat gccctggtat gactcaaacg ggttgcgctt
    34141 aacttttagc ccgcgcgatt ttattcaggt caatgcgggt gtgaaccaaa aaatggtagc
    34201 gcgtgcgttg gaatggctgg atgtgcaacc tgaagatcgc gtactggatc tgttctgcgg
    34261 tatgggcaac tttacactgc cattggcgac acaagctgcc agtgtggtgg gtgtagaagg
    34321 tgttccggcg ctggtggaaa aaggccagca gaatgcgcgt cttaatggct tacagaatgt
    34381 gacgttttat cacgaaaatc ttgaagaaga tgtcacaaag cagccgtggg cgaaaaacgg
    34441 cttcgataaa gtgttgctgg accccgcgcg agcaggtgcc gcaggtgtta tgcagcaaat
    34501 tataaaactg gaacctattc gtatagttta tgtatcctgt aatcctgcaa cgctggctcg
    34561 ggatagcgaa gcgttattaa aagcaggata taccattgcg cgactggcga tgctggatat
    34621 gttcccacac acgggacatc tggaatcgat ggtacttttc tcgcgcgtta aatagttacg
    34681 atttgccgat ttcggcaggt ctggtcccta aaggagagga cgatggttgc ggtaagaagt
    34741 gcacatatca ataaggctgg tgaatttgat ccggaaaaat ggatcgcaag tctgggtatt
    34801 accagccaga agtcgtgtga gtgcttagcc gaaacctggg cgtattgtct gcaacagacg
    34861 caggggcatc cggatgccag tctgttattg tggcgtggtg ttgagatggt ggagatcctc
    34921 tcgacattaa gtatggacat tgacacgctg cgggcggcgc tgctgttccc tctggctgat
    34981 gccaacgtag ttagcgaaga tgtgctgcgt gagagcgtcg gtaagtcggt tgttaacctt
    35041 attcacggcg tgcgtgatat ggcggcgatc cgccagctga aagcgacgca cactgattct
    35101 gtttcctccg aacaggtcga taacgttcgc cggatgttat tggcgatggt cgatgatttt
    35161 cgctgcgtgg tcatcaaact ggcggagcgt attgctcatc tgcgcgaagt aaaagatgcg
    35221 ccggaagatg aacgcgtact ggcggcaaaa gagtgcacca atatctacgc gccgttggcg
    35281 aaccgtcttg ggattgggca actgaaatgg gagctggaag attactgctt ccgttatctg
    35341 cacccgaccg aatacaaacg catcgcaaaa ctgctgcatg aacgccgtct cgaccgcgaa
    35401 cactatatcg aagagtttgt cggtcatctg cgcgctgaga tgaaagctga aggcgttaaa
    35461 gctgaagtgt atggtcgtcc gaaacacatc tacagcatct ggcgtaaaat gcagaaaaag
    35521 aacctcgcct ttgatgagct gtttgatgtg cgtgcggtac gtattgtcgc cgagcgttta
    35581 caggattgct atgccgcact ggggatagtg cacactcact atcgccacct gccggatgag
    35641 tttgacgatt atgtcgctaa cccgaaacca aacggttatc agtctattca taccgtggtt
    35701 ctggggccag gtggcaaaac cgttgagatc caaatccgca ccaaacagat gcatgaagat
    35761 gcagagttgg gtgttgctgc gcactggaaa tataaagagg gcgcggcagc tggcggcgca
    35821 cgttcgggac atgaagaccg gattgcctgg ctgcgtaaac tgattgcgtg gcaggaagag
    35881 atggctgatt ccggcgaaat gctcgacgaa gtacgcagcc aggtctttga cgaccgggtg
    35941 tatgtcttta cgccgaaagg tgatgtcgtt gatttgcctg cgggatcaac gccgctggac
    36001 ttcgcttacc acatccacag tgatgtcgga caccgctgca tcggggcaaa aattggcggg
    36061 cgcattgtgc cgttcaccta ccagctgcag atgggcgacc agattgaaat tatcacccag
    36121 aaacagccga accccagccg tgactggtta aacccaaacc tcggttacgt cacaaccagc
    36181 cgtgggcgtt cgaaaattca cgcctggttc cgtaaacagg accgtgacaa aaacattctg
    36241 gctgggcggc aaatccttga cgacgagctg gaacatctgg ggatcagcct gaaagaagca
    36301 gaaaaacatc tgctgccgcg ttacaacttt aatgatgtcg acgagttgct ggcggcgatt
    36361 ggtggcgggg atatccgtct caatcagatg gtgaacttcc tgcaatcgca atttaataag
    36421 ccgagtgccg aagagcagga cgccgccgcg ctgaagcaac ttcagcaaaa aagctacacg
    36481 ccgcaaaacc gcagtaaaga taacggtcgc gtggtggttg aaggtgtcgg taacctgatg
    36541 caccacatcg cgcgctgctg ccagccgatt cctggagatg agattgtcgg cttcattacc
    36601 caggggcgcg gtatttcagt acaccgcgcc gattgcgaac aactggcgga actgcgctcc
    36661 catgcgccag aacgcattgt tgacgcggta tggggtgaga gctactccgc cggatattcg
    36721 ctggtggtcc gcgtggtggc taatgatcgt agtgggttgt tacgtgatat cacgaccatt
    36781 ctcgccaacg agaaggtgaa cgtgcttggc gttgccagcc gtagcgacac caaacagcaa
    36841 ctggcgacca tcgacatgac cattgagatt tacaacctgc aagtgctggg gcgcgtgctg
    36901 ggtaaactca accaggtgcc ggatgttatc gacgcgcgtc ggttgcacgg gagttaggcc
    36961 gaaatttgct cgtatctaca atgtagattg atatatactg tatctacata tgatagcggt
    37021 ttgaggaaag ggttatgatc cacagtagcg taaagcgttg gggaaattca ccggcggtgc
    37081 ggatcccggc tacgttaatg caggcgctca atctgaatat tgatgatgaa gtgaagattg
    37141 acctggtgga tggcaaatta attattgagc cagtgcgtaa agagcccgta tttacgcttg
    37201 ctgaactggt caacgacatc acgccggaaa acctccacga gaatatcgac tggggagagc
    37261 cgaaagataa ggaagtctgg taatggtaag ccgatacgta cccgatatgg gcgatctgat
    37321 ttgggttgat tttgacccga caaaaggtag cgagcaagct ggacatcgtc cagctgttgt
    37381 cctgagtcct ttcatgtaca acaacaaaac aggtatgtgt ctgtgtgttc cttgtacaac
    37441 gcaatcaaaa ggatatccgt tcgaagttgt tttatccggt caggaacgtg atggcgtagc
    37501 gttagctgat caggtaaaaa gtatcgcctg gcgggcaaga ggagcaacga agaaaggaac
    37561 agttgcccca gaggaattac aactcattaa agccaaaatt aacgtactga ttgggtagtg
    37621 ttactaactc acttctattc tggtcacagg tttccacctg acagacccgc aattttcagg
    37681 acaattcaat gaatcaaatc gaccgtttgc tcactattat gcagcgcctg cgcgatccgg
    37741 aaaacggctg cccgtgggat aaagagcaga catttgccac cattgcgcct tacacccttg
    37801 aagaaaccta cgaagtgctg gacgccatcg cccgtgaaga ttttgacgat cttcgcggtg
    37861 aactgggcga tctgctattc caggtggtgt tttacgcgca aatggctcag gaagaagggc
    37921 gctttgactt taatgatatt tgcgctgcta ttagcgataa attagagcgt cgccatccgc
    37981 atgtttttgc tgacagttct gccgaaaaca gtagtgaagt gcttgcccgt tgggagcaaa
    38041 tcaaaaccga agagcgcgcg cagaaagcgc agcattcggc gctggacgat attcctcgta
    38101 gtttaccggc tttaatgcgg gcgcaaaaaa tccagaaacg ttgcgccaac gttggcttcg
    38161 actggacaac gcttggccca gtggttgata aagtctacga agagatcgac gaggtgatgt
    38221 acgaggcacg gcaggctgtt atcgaccagg ctaaactgga ggaggaaatg ggcgacctgc
    38281 tgtttgccac ggttaatctg gctcgccatt tagggacgaa agcagaaatc gcattgcaaa
    38341 aagcgaacga aaaattcgag cgtcgttttc gcgaagtgga gcgtattgtt gccgcgcgtg
    38401 gactggaaat gacaggtgtt gacctcgaaa caatggaaga agtctggcaa caggtaaaac
    38461 ggcaggaaat tgatctctaa gggaattacg cggtcaagcg cgatttgtgt cattttttaa
    38521 atgacaagcg cttgatttgc gtcaaaaaca tttaccccaa aggggctatt ttctcactcc
    38581 tgatttcaat agtgcgctgg cgaagaggag ggataatgaa agtttgtggc acaggtcatg
    38641 ttcgggtata ctgctttccc gtcctggtta ttccatcgtc ttttcaacct aacttctcag
    38701 gttcagcatg acaacgaact atatttttgt gaccggcggg gtcgtatcct ctctgggtaa
    38761 aggcattgcc gcagcctccc tcgcagccat tcttgaagcc cgtggcctca atgtgaccat
    38821 catgaaactg gatccgtaca tcaacgtcga tccaggtact atgagcccaa tccaacacgg
    38881 ggaagtgttc gttactgaag acggcgctga aaccgacctg gacctggggc actacgagcg
    38941 tttcattcgt accaaaatga gccgccgcaa caacttcacc acgggtcgta tctactctga
    39001 cgttctgcgt aaagaacgcc gcggtgacta cctcggcgca accgtgcagg ttattccgca
    39061 catcactaac gcaatcaaag agcgcgtgct ggaaggtggc gaaggtcatg acgtagtact
    39121 ggtagaaatc ggcggtacag taggtgatat cgaatccttg ccgttcctcg aagcgattcg
    39181 ccagatggct gttgaaattg gccgtgagca cactctgttt atgcacctga cgctggtgcc
    39241 gtacatggca gcgtctggtg aagtcaaaac caaaccgact cagcactctg taaaagagct
    39301 gctctccatc ggtatccagc ctgacatcct gatttgtcgt tcagatcgcg ctgttccggc
    39361 gaacgaacgt gcgaagattg cattgttctg taatgttccg gaaaaagcgg ttatttctct
    39421 gaaagacgtc gattccatct ataaaattcc gggcctgttg aaatctcagg ggctggacga
    39481 ttatatttgt aaacgattca gcttaaactg cccggaagcg aatctgtccg aatgggaaca
    39541 ggttatcttc gaagaagcga acccggtaag tgaagtcacc atcggtatgg tcggcaagta
    39601 cattgaactg ccggatgctt ataaatcagt gatcgaagca ctgaaacacg gtgggctgaa
    39661 gaatcgtgtc agcgtcaaca tcaaactgat cgattcacaa gatgttgaaa cgcgcggcgt
    39721 tgaaatcctt aaaggtctgg acgcaatcct cgtacctggc ggtttcggct atcgtggcgt
    39781 agaaggcatg attacgaccg cgcgttttgc gcgtgagaac aatattcctt atctgggcat
    39841 ttgcctgggt atgcaggtgg cgttaattga ttacgctcgc catgttgcca acatggagaa
    39901 cgccaactct acggaatttg tgccagactg taagtacccg gttgtggcgc tgattaccga
    39961 gtggcgcgat gaaaacggca acgttgaagt tcgtagcgag aagagcgatc tcggcggtac
    40021 catgcgtctc ggcgcacagc agtgccagtt ggttgacgat agcctggttc gccagctgta
    40081 caatgcgccg acaattgttg agcgtcatcg tcaccgttac gaagtcaaca acatgctgtt
    40141 gaaacagatt gaagatgcag gtctgcgcgt tgcgggccgt tccggggatg atcagttggt
    40201 cgagatcatc gaagttccga atcacccgtg gttcgtggct tgccagttcc atccggagtt
    40261 tacttctact ccacgtgatg gtcacccgct gtttgcaggc tttgtgaaag ccgccagcga
    40321 gttccagaaa cgtcaggcga agtaagtaaa aaagttagaa cggcaacgcg taccctgggt
    40381 acgcgttgtt tgtctggagt ttcagtttaa ctagtgactt gaggaaaacc taatgtccaa
    40441 aatcgtaaaa atcatcggtc gtgaaatcat cgactcccgt ggtaacccga ctgttgaagc
    40501 cgaagtacat ctggagggtg gtttcgtcgg tatggcagct gctccgtcag gtgcttctac
    40561 tggttcccgt gaagctctgg aactgcgcga tggcgacaaa tcccgtttcc tgggtaaagg
    40621 cgtaaccaaa gctgttgctg cggtaaacgg cccgatcgct caggcgctga ttggcaaaga
    40681 tgctaaagat caggctggca ttgacaagat catgatcgac ctggacggca ccgaaaacaa
    40741 atccaaattc ggcgcgaacg caatcctggc tgtatctctg gctaacgcca aagctgctgc
    40801 agctgctaaa ggtatgccgc tgtacgagca catcgctgaa ctgaacggta ctccgggcaa
    40861 atactctatg ccggttccga tgatgaacat catcaacggt ggtgagcacg ctgacaacaa
    40921 cgttgatatc caggaattca tgattcagcc ggttggcgcg aaaactgtga aagaagccat
    40981 ccgcatgggt tctgaagttt tccatcacct ggcaaaagtt ctgaaagcga aaggcatgaa
    41041 cactgctgtt ggtgacgaag gtggctatgc gccgaacctg ggttccaacg ctgaagctct
    41101 ggctgttatc gctgaagctg ttaaagctgc tggttatgaa ctgggcaaag acatcactct
    41161 ggcgatggac tgcgcagctt ctgaattcta caaagatggt aaatacgttc tggctggcga
    41221 aggcaacaaa gcgttcacct ctgaagaatt cactcacttc ctggaagaac tgaccaaaca
    41281 gtacccgatc gtttctatcg aagacggtct ggacgaatct gactgggacg gtttcgcata
    41341 ccagaccaaa gttctgggcg acaaaatcca gctggttggt gacgacctgt tcgtaaccaa
    41401 caccaagatc ctgaaagaag gtatcgaaaa aggtatcgct aactccatcc tgatcaaatt
    41461 caaccagatc ggttctctga ccgaaactct ggctgcaatc aagatggcga aagatgctgg
    41521 ctacactgca gttatctctc accgttctgg cgaaactgaa gacgctacca tcgctgacct
    41581 ggctgttggt actgctgcag gccagatcaa aactggttct atgagccgtt ctgaccgtgt
    41641 tgctaaatac aaccagctga ttcgtatcga agaagctctg ggcgaaaaag caccgtacaa
    41701 cggtcgtaaa gagatcaaag gccaggcata agactgactt aacgtcttaa taaaaaaccc
    41761 gcttcttgaa gcgggttttt ttatcttcac attttgagtt tagtaagcag agcgataaca
    41821 ttaataaatg ttattcccac caggataagt atccgaacaa tttttctgct attccttaca
    41881 ttgctgacaa aatcattatg agaaatattg gatgaatatt ttcgttgatg gactttataa
    41941 tcccaaagaa aatcatttgc ctcaggtatt gggttaatca gactgtagat gcaaaagtac
    42001 acaaacgtat ctaaagcaag cagtgatgtt atcagttgaa ataattcatc gccctggaag
    42061 gctttattaa gccaaatcat tccagcagca ccgaataagc caaatacaaa tttacttaat
    42121 cgtggatgat tcgctaaaat cttactcata tttccttacc ttccctgtaa tctgttttta
    42181 agtcttacca tcttcctgac gcgccgccac cgccggatga accgccacca ccgctgaaat
    42241 tgtcattgtt gaaagaggat gaagaggaag atgacgatga tgaagaatgt cgggaggccg
    42301 agttgcttct ttcgtaacgt gacggtcggt tggcgctacg cattgaatca cgcacctttc
    42361 tgccgaaagg cgtgaaagcg atagctacga ccaggaatgg agtggcgaaa cataataatg
    42421 tggcaagaac tccccagctc ccgctatatc cagcgatggg aagtacaaag gccagtatta
    42481 tggcagcaaa gcaaatcact cccagcgttt tgacatatcc tctggcagaa ataaacgtca
    42541 gtactatcgc ccagactaac agagcccacc aaccgctaaa gggtaaagga tgatcgggcg
    42601 atgtattatt gactaaatca ttttctgata atcgggtttc aatatcacca ataccttgtt
    42661 gcaagccgac agcaagatcg ccctttttaa acgcaggaat aatactgttg cgtataattt
    42721 gcccggactg cgcatccgtc agaataccct caagtccgta gccaatttca atatgtactg
    42781 tatgatcttg ccaggcaact accagtaaaa taccgtcatc aagattctta tgaccgggct
    42841 gccaacgctc gaatactcgt gttgcatatt gctcgatggt ttcatcgcct gttgtttcta
    42901 ctactaacac gatcagttgt gcgtggcttt tttgcgtcag ggttttaatc tgactggtca
    42961 gcgtacttac ttcctccctg gttaatgtcc ctgttaaatc atttatatac ttagcgaatt
    43021 caggtactgg aacttgcact gcaaacgcag atgaacagat cagaacaaga agtaagatta
    43081 aatttttcat ataattaact cgcctaatga gcactagcat tctggctaaa atctattgtt
    43141 ggtgctttac tcacagcggc gacatcatcc ggcaaataat ttttcttcgg tgtgtaatcc
    43201 atgacctttg cggttaatac agcggggaat ttgcggatag taacattata ttgttcaatg
    43261 gctttaatat atcttcctcg cgcaacggca attcgatttt cacttccttc gagctgtacc
    43321 attaagtttt gatagagctc ttgagccttt aactcagggt aacgctcact aattatcgtt
    43381 aactgaccga gagtgcgagt cacctgcgct tgagcctgtt gccaggcttg taatttttgt
    43441 tcatttcccg gagtttgctg aagatcgctg ctggcgcgat tagcctggct acgagccaat
    43501 gttacggctt ccaaaacgtc tttttcatgg ttggaataac ctttaatact ggcgacaaga
    43561 ttaggaataa gatcggcgcg acgttgatat tgatttaata cctcagacca ggaggcgtta
    43621 acctgctcat cataggtttg aatatcgtta tatccacagc ctgataaatt gaaaataaat
    43681 ataattacga tgaatgtcct gagcatgttt aa
//
LOCUS       DAPGWD010000025        41182 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPGWD010000025 DAPGWD010000000
VERSION     DAPGWD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.025"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..121
                     /locus_tag="RHX88_001282"
     CDS             <1..121
                     /locus_tag="RHX88_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2229997.1"
                     /translation="TLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
     gene            108..332
                     /locus_tag="RHX88_001283"
     CDS             108..332
                     /locus_tag="RHX88_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDV2229998.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(399..1121)
                     /gene="sdiA"
                     /locus_tag="RHX88_001284"
     CDS             complement(399..1121)
                     /gene="sdiA"
                     /locus_tag="RHX88_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDV2229999.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1351..2103)
                     /gene="yecC"
                     /locus_tag="RHX88_001285"
     CDS             complement(1351..2103)
                     /gene="yecC"
                     /locus_tag="RHX88_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDV2230000.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(2100..2768)
                     /gene="tcyL"
                     /locus_tag="RHX88_001286"
     CDS             complement(2100..2768)
                     /gene="tcyL"
                     /locus_tag="RHX88_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDV2230001.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2783..3769)
                     /gene="dcyD"
                     /locus_tag="RHX88_001287"
     CDS             complement(2783..3769)
                     /gene="dcyD"
                     /locus_tag="RHX88_001287"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDV2230002.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3874..4674)
                     /gene="tcyJ"
                     /locus_tag="RHX88_001288"
     CDS             complement(3874..4674)
                     /gene="tcyJ"
                     /locus_tag="RHX88_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2230003.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4762..5313)
                     /gene="fliZ"
                     /locus_tag="RHX88_001289"
     CDS             complement(4762..5313)
                     /gene="fliZ"
                     /locus_tag="RHX88_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDV2230004.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5359..6078)
                     /gene="fliA"
                     /locus_tag="RHX88_001290"
     CDS             complement(5359..6078)
                     /gene="fliA"
                     /locus_tag="RHX88_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDV2230005.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDITDYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6243..7706)
                     /locus_tag="RHX88_001291"
     CDS             complement(6243..7706)
                     /locus_tag="RHX88_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDV2230006.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRVRELTVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGDAYTVNVDSGAVKDTT
                     GNDIFVSAADGSLTTKSDTNIAGTGIDATALAAAAKNKAQNDKFTFNGVEFTTTTAAD
                     GNRNGVYSAEIDGKSVTFTVTDADKKASLITSETVYKNSAGLYTTTKVDNKAATLSDL
                     DLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNEDAAK
                     SLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDAD
                     YATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            7961..9373
                     /gene="fliD"
                     /locus_tag="RHX88_001292"
     CDS             7961..9373
                     /gene="fliD"
                     /locus_tag="RHX88_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDV2230007.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDSAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            9388..9798
                     /gene="fliS"
                     /locus_tag="RHX88_001293"
     CDS             9388..9798
                     /gene="fliS"
                     /locus_tag="RHX88_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDV2230008.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            9798..10163
                     /gene="fliT"
                     /locus_tag="RHX88_001294"
     CDS             9798..10163
                     /gene="fliT"
                     /locus_tag="RHX88_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDV2230009.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10241..11728
                     /gene="amyA"
                     /locus_tag="RHX88_001295"
     CDS             10241..11728
                     /gene="amyA"
                     /locus_tag="RHX88_001295"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV2230010.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(11762..12175)
                     /locus_tag="RHX88_001296"
     CDS             complement(11762..12175)
                     /locus_tag="RHX88_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2230011.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12362..13567
                     /gene="yedE"
                     /locus_tag="RHX88_001297"
     CDS             12362..13567
                     /gene="yedE"
                     /locus_tag="RHX88_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDV2230012.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            13564..13797
                     /gene="yedF"
                     /locus_tag="RHX88_001298"
     CDS             13564..13797
                     /gene="yedF"
                     /locus_tag="RHX88_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDV2230013.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13904..14572
                     /locus_tag="RHX88_001299"
     CDS             13904..14572
                     /locus_tag="RHX88_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDV2230014.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNIKNQGAEL
                     IQPV"
     gene            complement(14967..15281)
                     /gene="fliE"
                     /locus_tag="RHX88_001300"
     CDS             complement(14967..15281)
                     /gene="fliE"
                     /locus_tag="RHX88_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDV2230015.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            15496..17154
                     /gene="fliF"
                     /locus_tag="RHX88_001301"
     CDS             15496..17154
                     /gene="fliF"
                     /locus_tag="RHX88_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDV2230016.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            17147..18142
                     /gene="fliG"
                     /locus_tag="RHX88_001302"
     CDS             17147..18142
                     /gene="fliG"
                     /locus_tag="RHX88_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDV2230017.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQITNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            18135..18821
                     /gene="fliH"
                     /locus_tag="RHX88_001303"
     CDS             18135..18821
                     /gene="fliH"
                     /locus_tag="RHX88_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDV2230018.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            18821..20194
                     /gene="fliI"
                     /locus_tag="RHX88_001304"
     CDS             18821..20194
                     /gene="fliI"
                     /locus_tag="RHX88_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDV2230019.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            20213..20656
                     /gene="fliJ"
                     /locus_tag="RHX88_001305"
     CDS             20213..20656
                     /gene="fliJ"
                     /locus_tag="RHX88_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDV2230020.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            20653..21780
                     /gene="fliK"
                     /locus_tag="RHX88_001306"
     CDS             20653..21780
                     /gene="fliK"
                     /locus_tag="RHX88_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDV2230021.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            21885..22349
                     /gene="fliL"
                     /locus_tag="RHX88_001307"
     CDS             21885..22349
                     /gene="fliL"
                     /locus_tag="RHX88_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDV2230022.1"
                     /translation="MTDYATSKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            22354..23358
                     /gene="fliM"
                     /locus_tag="RHX88_001308"
     CDS             22354..23358
                     /gene="fliM"
                     /locus_tag="RHX88_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDV2230023.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            23355..23768
                     /gene="fliN"
                     /locus_tag="RHX88_001309"
     CDS             23355..23768
                     /gene="fliN"
                     /locus_tag="RHX88_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDV2230024.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            23771..24136
                     /gene="fliO"
                     /locus_tag="RHX88_001310"
     CDS             23771..24136
                     /gene="fliO"
                     /locus_tag="RHX88_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDV2230025.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            24136..24873
                     /gene="fliP"
                     /locus_tag="RHX88_001311"
     CDS             24136..24873
                     /gene="fliP"
                     /locus_tag="RHX88_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDV2230026.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            24883..25152
                     /gene="fliQ"
                     /locus_tag="RHX88_001312"
     CDS             24883..25152
                     /gene="fliQ"
                     /locus_tag="RHX88_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDV2230027.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            25161..25946
                     /gene="fliR"
                     /locus_tag="RHX88_001313"
     CDS             25161..25946
                     /gene="fliR"
                     /locus_tag="RHX88_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDV2230028.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFSAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            26236..26859
                     /gene="rcsA"
                     /locus_tag="RHX88_001314"
     CDS             26236..26859
                     /gene="rcsA"
                     /locus_tag="RHX88_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDV2230029.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(26903..27091)
                     /gene="dsrB"
                     /locus_tag="RHX88_001315"
     CDS             complement(26903..27091)
                     /gene="dsrB"
                     /locus_tag="RHX88_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDV2230030.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            27254..27481
                     /gene="yodD"
                     /locus_tag="RHX88_001316"
     CDS             27254..27481
                     /gene="yodD"
                     /locus_tag="RHX88_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDV2230031.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            27779..28594
                     /locus_tag="RHX88_001317"
     CDS             27779..28594
                     /locus_tag="RHX88_001317"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDV2230032.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(28591..30285)
                     /gene="yedQ"
                     /locus_tag="RHX88_001318"
     CDS             complement(28591..30285)
                     /gene="yedQ"
                     /locus_tag="RHX88_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDV2230033.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(30523..30705)
                     /locus_tag="RHX88_001319"
     CDS             complement(30523..30705)
                     /locus_tag="RHX88_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDV2230034.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(30784..31701)
                     /locus_tag="RHX88_001320"
     CDS             complement(30784..31701)
                     /locus_tag="RHX88_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDV2230035.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            31874..32794
                     /gene="yedA"
                     /locus_tag="RHX88_001321"
     CDS             31874..32794
                     /gene="yedA"
                     /locus_tag="RHX88_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDV2230036.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(32783..33253)
                     /gene="vsr"
                     /locus_tag="RHX88_001322"
     CDS             complement(32783..33253)
                     /gene="vsr"
                     /locus_tag="RHX88_001322"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDV2230037.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(33234..34652)
                     /gene="dcm"
                     /locus_tag="RHX88_001323"
     CDS             complement(33234..34652)
                     /gene="dcm"
                     /locus_tag="RHX88_001323"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDV2230038.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(34719..35157)
                     /locus_tag="RHX88_001324"
                     /pseudo
     CDS             complement(34719..35157)
                     /locus_tag="RHX88_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            35213..35910
                     /locus_tag="RHX88_001325"
                     /pseudo
     CDS             35213..35910
                     /locus_tag="RHX88_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            35926..36654
                     /gene="hchA"
                     /locus_tag="RHX88_001326"
                     /pseudo
     CDS             35926..36654
                     /gene="hchA"
                     /locus_tag="RHX88_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
     gene            complement(36762..38120)
                     /gene="hprS"
                     /locus_tag="RHX88_001327"
     CDS             complement(36762..38120)
                     /gene="hprS"
                     /locus_tag="RHX88_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDV2230039.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYKKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(38120..38791)
                     /gene="hprR"
                     /locus_tag="RHX88_001328"
     CDS             complement(38120..38791)
                     /gene="hprR"
                     /locus_tag="RHX88_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDV2230040.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            38924..39337
                     /gene="hiuH"
                     /locus_tag="RHX88_001329"
     CDS             38924..39337
                     /gene="hiuH"
                     /locus_tag="RHX88_001329"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDV2230041.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            39446..40450
                     /gene="msrP"
                     /locus_tag="RHX88_001330"
     CDS             39446..40450
                     /gene="msrP"
                     /locus_tag="RHX88_001330"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDV2230042.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            40451..41086
                     /gene="msrQ"
                     /locus_tag="RHX88_001331"
     CDS             40451..41086
                     /gene="msrQ"
                     /locus_tag="RHX88_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDV2230043.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLALLLLALRYKKLRSLFNRLRKQVHNKLSV"
BASE COUNT        10232 a        10534 c        10219 g        10197 t
ORIGIN      
        1 cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact ccgccgcctt
       61 aacatttttc accgcaaatt ttcattgcaa catgacgagg aaaataaatg agtacgcctg
      121 atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc ctgaaagcga
      181 tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg atgttaaaga
      241 tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt tccaaaacgg
      301 ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgagag cgtgcctttc
      361 agccggtttt tgcatctggc acgcagaaag aaaagcgatc aaattaagcc agtagcggcc
      421 gcgtaacagg ccacctgggt cttatttggt gcattgattt ttttctgcat gtttttctga
      481 tggaaattga ccgtattctc agagattgac aaaatcatcg ctatctctgc tgatgtcttc
      541 ccttccgccg tccatttcag aatttctttt tcgcgcttgc tgaaattcat ctctggcgtc
      601 atcactattt catcatttaa acgcatcaga gccatcagac tttcgcgcac cagtaactgc
      661 atttttaatt gcagttcatc actaagaatg ggtatttcgc gcgtgctgca acgggaaaag
      721 gacaaaaagc ccagcgcccg atttggcagc attaaatact gagtgacacc gcggcgtaaa
      781 ccatgtgcgt gcgcggcttc ccataatggc tgtgcttcgc tgaataagtc atcattccac
      841 attaaatggc cctgactaaa gttttcaggg ttcagcacag gatcaattgc gagaaaattt
      901 tttgcctgat aataactaac ccacgactca gggtaattgg tgtagaaagc cactttaggt
      961 cgcgtgaatg gtaccgggtg gcgaacacat aacgaatagt aatcgtactc cagctgctga
     1021 gcctgaagct ctatttcatg gtagacctct tctgcggcct ccatcctctg aaaacgcaac
     1081 agcatcgtgc gacgccagct gaaaaaatcc gtatcctgca tagtaaaccg caacgcccct
     1141 gagagtgagt atcatttata atgataattc aaactatcac ataaataaga tttatatata
     1201 atttatatta ttcaggcaat gaattacttt tgcaagccat cgcattctct tatgttatta
     1261 atgagttatg ctgatttgtt aagcagtttt atcacgcttg aaatggcgtc ccgccccgac
     1321 aggtgaatcg tcggggctga ttttttctta ttattgcagc agaaacttct cgaggaactg
     1381 tcgggtacga ggctgctggg gatcggcaaa taacgctttt gctgccccct gctcgactat
     1441 ccgcccctgg tccataaaaa tcgcccggtc cgcaacatcc cgggcaaagc tcatttcgtg
     1501 cgtcacaatc accatcgtgc gcttttcctg cgccagctgg cggattgtgt tcaggacttc
     1561 gcccaccagc tctggatcca gcgctgacgt tggctcgtca aacaaaatca cctcaggacg
     1621 cattgccagc gcacgcgcaa tcgcaacacg ctgctgttga ccgccagaca aacgacgtgg
     1681 atagctggtt tctttaccgg ccagcccaac tttcgccagc agctcgcgag cgcgcgccgt
     1741 ggcctcttct ttcggttcgc ctttgacgat caccggccct tcaataatgt tttccagcac
     1801 cgtacgatgc ggaaacaaat taaagttctg gaagacaaac ccgacgtgct gacgcaactg
     1861 gcgaatcagc gatttttgct gacttaatga acgtgcagta tcaatagtga tatcgccaac
     1921 ggtgatcgtt cccgcttccg gttgttccag cagatttatg ctgcgtagca acgtggtttt
     1981 gccggaacca ctcggaccga taattgccac cacttcgcca ggctttacct cgaggtcgat
     2041 accgtgcagc accgtctgac cgtgaaattt tttcaccaag ttcttaactt caatggcact
     2101 cattttggtt ctctctcctg gcgattaagt tgattctcaa aatggttctg caacgtcgat
     2161 aatactgtcg ccatgatcca gtagatgagc gaagccgcca ggtacatggt gaacacctcc
     2221 agcgtgcgcg aggtaatcaa ctgcgcctga cggaacagct ccggcacctg gattgtcgcg
     2281 gccagcgacg tatcctttac caggctgata aagctgttcg acagcggcgg caacgccacc
     2341 cgcgccgcct gtggcaaaat cgcgcgacgc atagtctgcc atggcgtcat accaatactg
     2401 gcagctgctt cccactgacc tttatcaata gaagaaatgg cggcgcgcag tgtttcggcg
     2461 gcataggcgg cagtgttcag cgacagacca atcattgctg acggaatggg atctaattcg
     2521 ataccaaact gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag cggtgtacca
     2581 cgaaaaatag agatataaaa acgcgccagc cagcgcacgg gccagattgg cgacaggcgc
     2641 atcagcgcga gaataaaccc gagcagtaag ccaaaaaaca tgccgccgat actgagttgc
     2701 agcgtatacc cagccccttt cagcaggaac ggcaaggaat caataaccag ttgtatactt
     2761 tcttgcatga gcgttcccgg agctaaacgt ggggatgata ggcgaacagc gcaggcgcac
     2821 cgccggtatg aataaacaga atcggccctt catctttgaa gcgtttctga ctgataccgt
     2881 caatcagccc cgccatcgct tttccggtat acacaggatc aagcagaatg ccttccagcc
     2941 gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg cacgccgtag ccaggtgcaa
     3001 aatagtcatc ccagagtata atttccgctg atgcggtcag ctccagttct ttcgcaatcg
     3061 cctgttgtag gttaaccact ttcggcaact gatcggcaac ggaacgcgac acggtcacgc
     3121 caatcagttc gctttcaggc atcaggtgtt ccagcccaac agccagtccg gcgtgagttc
     3181 cggcactgcc cgatgcgact accaccgacg aaatattaac cgccccttca cactgttgcg
     3241 cgatttccag cgcactctcc acataaccta gcgcgccaag agcattagaa ccgccaaccg
     3301 gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt cgccagctct tccagttggg
     3361 cattgggatc ggtcagtgct tcgcacattt caatctgggt attgaacaga tccagcaaca
     3421 agcgattgcc gttggttaaa tagttttctg cggttgtgcc aataggattt tccagcagcg
     3481 ccacacagtg cagaccgagt ttcgccgcaa ctgcggcagt ctgccgcaca tggttagact
     3541 ggatcgcccc ggcagtaatc agcgtatcgg caccttcgcg cagagcatct gctgcgagaa
     3601 attccagctt acgtaattta ttgccgccca ttgccatggg ggtgacgtca tcccgtttga
     3661 tgaaaatttc ccgtccaaga taatcagaaa agcgcggcag atattcgagc ggtgttggcg
     3721 cgccgataaa ctccaaccgt ggaaaacggg ttaaattatg cagtggcata acagcctccg
     3781 atgtgtgttg ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc
     3841 tggtgaaagc gccctttttt gtcattatgc tgattatttg gtcacatcag caccaaacca
     3901 tttttcggaa agggcttgca gagtgccatc tttttgcatt tccgcaattg catcgttcac
     3961 tgctttcagc aggtcttcat ttcctttacg cagcgccacg ccagactcct gacgggagaa
     4021 tgcttcaccg gttactgcca gcgtatcgtt ggttttcttc accagatcca gcgccgccag
     4081 acggtcaacg aggatcgcat cgatacggcc tacgcgcaga tcctgatatt tggtcgggtc
     4141 atcatcatag gtacgcacat cgacgccctg aacattctgc cgcagccact cttcatagtt
     4201 ggtgcccaga ccgacaccca cttttttgcc tttcagatca gcagccgttt taatggtgcc
     4261 ttcgttacct tttttcacca gcgcctgaat accagaaatg gtgtacgggg tagagaaatc
     4321 gtattttttc ttgcgctcat cagaaatggt gacctgatta atcaccacat caatgcgttt
     4381 agagtccagc gacgccagca taccgtccca tttggtcggt tttagtgacg cctcaacgcc
     4441 aagatgtttt gccagctgtt gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc
     4501 cccctgaaaa ctgaacggcg gataagtgcc ttccagccct accagcagcg tgccgcgctc
     4561 tttaacttta ttaagcagac cttcatctgc aaaactttta acgctcatgc ccgcaaccag
     4621 cgctacggcc atcacactca tcaatgcctg acgtcccaga tgtgctaatt tcatatttac
     4681 cccgaatgtt gttatgtctg tttgcagtgt agagccattg tttgtaaaca caaaaacaac
     4741 tccgctacat cttattctta tttaatatat atcagaagca ggcaggctgg aggatttctg
     4801 cacaagtccg gtacacgttt gacgctgata gtgttgatac ttccgtaatg cgatgcggta
     4861 attgttggtg ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga aaccgtcctg
     4921 cagcggatcg agggaaatat tttgctcgtg tagatgattc cccaaacggc gcaaacgaac
     4981 gacatattca cgcaccgtac ctgggctcat ttcagtttgc tcaaaaagaa attgcttaaa
     5041 gccgataata tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc aaaatcggca
     5101 caatgccgcc catgattttt gttccgtttg ccagccattt tcatcaagca gcgcgtccag
     5161 gcgggaaatc tcgattttat tcactatttt gccgtcgcga acgagggtaa tgcgatcgag
     5221 taatttacgg caatgcgcgc aatgagtctg gctgtgttta aagtctttaa gatagcggct
     5281 taatggccgt cttttcaggt gctgcaccat cattaagaac tcctggtagt caaagttaaa
     5341 gtgcggcatt tactgacgtt ataacttacc cagtttagtg cgtaaccgtt taatagcctg
     5401 gctgtgtaac tgactgaccc gcgattcccc gacctccagc accgcgccaa tctctttgag
     5461 attcagctct tcctggtaat agagggttaa taccagtttt tcgcgctccg gcaacgtttc
     5521 gatggcttcc atcacccgct gacgcagatt actgtccagc agttgttgta gcgggttttc
     5581 tcgctgatga tcatcagtaa ccagttcgat gctatcgccg tgctcttcgc gccactcatc
     5641 gtaggagaag agctggctgt tattggtgtc gagcaacatt tggcgataat cggtaatatc
     5701 gatccctaaa cgttccgcta cctcagtttc cgtggcgttg cggccaagtt cctgctccag
     5761 ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg gcacccagtc
     5821 acggctgcga agttcatcca gcatagcgcc acggatacgc tgcactgcgt aagttgtaaa
     5881 tgccgttcct tgtagggcgt catagcgttc gacggcatta agtaacccaa tgccgcccgc
     5941 ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc gcaatgcttc
     6001 gtgacgcacc agcgggacat aacgctgcca tagcgagtgt ttatccatta caccttcagc
     6061 ggtatagagt gaattcacga taaacagccc tgcgttatat gagttatcgg catgattatc
     6121 cgtttctgca gggtttttaa tcggacgatt agtgggtgaa atgaggggtt atttgggggt
     6181 taccggtaaa ttgcgggcag aaaaaacccc gccggtggcg ggggttgagc gataagtgta
     6241 aattaacgca gcagagacag tacgttctgc gtggtctggt tagcctgcgc cagaacagag
     6301 gtacccgctt gttgcaggat ctgcgcacga gacatgttag acacttcggt cgcgtagtca
     6361 gcatcttcga tacggctacg ggcagaagac aggttgttta cggtgttgcc aaggttggtg
     6421 atagcagagt cgaaacggtt ttgtactgca ccgaggtcag aacgcagatt gtcaacttta
     6481 gccaatgctt tgtcgatagt ttcgagcggg ttggtggtag attgcaacga ttttgctgca
     6541 tcttcgttta ccagaatcgg gctaccacct tctgatttgc tcagatacat gactttattg
     6601 ttaccagaag cagtctccgt tatcgtttta ccatctgcac taacatcgta agttgcaccg
     6661 ttaacaacta acgtgcttcc tgttttctta gctgcattga gatcaagatc ggaaagtgtg
     6721 gcagccttgt tatcaacttt ggttgtcgta taaaggccag cgctattttt gtaaactgtc
     6781 tcactcgtaa tcaaagaagc ttttttgtca gcatctgtca cagtaaatgt cactgactta
     6841 ccatcaattt ctgcagaata tacaccattc ctattgccat ccgctgcagt tgttgttgtg
     6901 aattcaactc cattaaacgt gaatttatca ttctgtgctt tattcttagc cgctgctgcg
     6961 agtgctgtag catcaatccc tgtaccagct atgtttgtgt cagatttagt tgtcagtgaa
     7021 ccatctgctg cactaacaaa aatatcattc cctgtagtat ctttaacagc tccgctatct
     7081 acgttaacag tataagcatc accgccaaca tcatagttat cagtacctgt cgctttaaat
     7141 ttagaaatca ggtcactgcc agttgctttc tgcgcgccat cgatattaaa accgtccagg
     7201 ccgagagttt tcgcatcaat ttttgccaga ttgatagtga tggtttcacc atcattagca
     7261 ccaacctgaa ttttcatttc attattttca gcaaggactt tcacgccgtt aaactgagtt
     7321 tgctcagata cacggtcaat ttcttccaga cgttgagtaa tttcagcctg gatagaagaa
     7381 agatcgctgt cagagttagt accgttagtt gcctgaacag tcagttcacg tacacgctgc
     7441 aggttgttgt taatttcatt cagcgcacct tcagtggtct gcgcaacaga aataccatca
     7501 ttcgcgttac gggaagcctg agtcagacct ttaatatttg ctgtaaaacg gttagcaatc
     7561 gcctgacctg ctgcgtcatc tttagcactg ttaatacgca ggccagaaga gagacgttca
     7621 atggcggagc tcagagaaga ctgagatttg ttcaggttat tctgggtcaa cagcgacagg
     7681 ctgtttgtat taatgacttg tgccatgatc ttttccttat caattacaac ttgatgttat
     7741 tgggctgttg cccacggttt ctcaccgtac cccttgtatc ggcacctgaa tttcgaactt
     7801 tagaaaattt tagtttctct cctgtttttt cttaacggct gaaatatccg taatatacaa
     7861 ctctactctg cgcatcatct tcgtgaatca actattaaac tttgcgcaca aattgccgat
     7921 aacccgctta tctactgttt gcaatcaaaa ggaattaggt atggcttcat ttacatctct
     7981 tggcgttggt tcgaatctgc cgctggatac attgctaaac aacctgacca tcgctgaaaa
     8041 aaaacgctta aacccaatta ctcagcaaca gagtgacaat accgcacgtc tgacagccta
     8101 tggcacatta aaaagtgcat tagaaaaatt ccagactgca aatactgcgt taaataaagc
     8161 cgatctgttc aaaagcacta ccgttaccag tagcaatgaa gaccttaaag tttccaccga
     8221 agcaggtgct gcccccggta tctataccat cagcgtaacc caactggcgc aggcgcaatc
     8281 tttacgaact gattccccga caatcattgc atcaacaaaa gatgcactgg gtgatgagtc
     8341 atcagataca cgcacaataa aaattaccca agacggtcgt aaagagccgc tggaaatcaa
     8401 gctcaataaa gatcagacgt cattggatga gatcagcaaa gccattaacg atgccgatag
     8461 tggaatttcc gctagtatcg ttaaagtcaa agatggtaac tatcaattgg tattgaccgc
     8521 cagtgaaggt ctggccaata aaatgaccat atctgttgag ggtgacagta aattaaatga
     8581 tctgctggct tatgacagca agaccaacac cgggaatatg aaggaactgg ttaacgcgca
     8641 aaatgcacag ctaaacgtta acggtatcga tattgaacgt tcaagtaaca aaattaccga
     8701 tgctcctcag ggagtgactc tcgatttgac caaaaaagtc actgacgtcc gggtcacggt
     8761 gacaaaaagc aacgataaag caaccgaagc aattaaaggc tgggtggatt catataactc
     8821 cctcattgac acctttaaca ccctgacaaa atataaagaa gtagacccag gtgctgaagc
     8881 acaagataaa aataatggtg cacttctcgg agacagcgtt gtacgtacaa ttcaaagcgg
     8941 catccgcgca caatttgcaa acggtgccag cgacagtgcc ttcaaaacat taaacgagat
     9001 cgggattaag caggacggca cgacgggtaa gctgaaaatt gatgatgata aactcaaaaa
     9061 agtcctgaat gaaaatacag catccgtacg tgaattgctg gtaggcgatg gtaaagaaac
     9121 aggaattacc accaaaattg ccaccgaggt aaaaggttac cttgccgatg atggcattat
     9181 tgatagcgca caggacagta ttaacgccac actgaaaaaa ctgaccaaac agtatctatc
     9241 cgtcagtgcc agcattgatg acaccgtggc gcgttacact gcacagttta cgcaattaga
     9301 taccatgatg agcaaactga ataacaccag tacctatctc agccaacaat ttactgcaat
     9361 gagtaattcc tgatcacgga ggtcagcatg tataccgcga gcggctcaaa agcctatgca
     9421 caaattggcg tcgaaagcgc cgtaatgagc gccagccagc agcagctggt caccatgcta
     9481 tttgatggag tgctgagcgc actggttaga gcacgcctgt ttatgcagga caacaatcag
     9541 caaggcaaag gcgtctcttt gtcaaaagcg atcaacatca ttgagaacgg actgcgggtg
     9601 agtcttgatg aagagagcaa agacgaatta acccaaaact tgattgctct ttatagctat
     9661 atggtcaggc gcttgctgca agccaattta cgcaacgatg tctccgcagt cgaagaagtg
     9721 gaagcattaa tgcgcaatat tgccgatgcc tggaaagagt cgttactctc cccttctttg
     9781 attcaggacc cagtctgatg aaccacgcac cgcatttgta tttcgcctgg caacaactcg
     9841 tcgaaaaaag ccagctcatg ttacgcctgg caacagaaga acaatgggac gaactcatcg
     9901 ccagcgagat ggcgtatgtg aatgcggtgc aggagattgc acatctgact gaagagattg
     9961 acccgtccac cacgatgcag gagcagctcc gcccgatgct gcgcctgatt ctcgacaacg
    10021 aaagcaaggt aaagcagtta ttacagattc ggatggatga actggcgaaa ctggtcggtc
    10081 agtcatcggt gcaaaaatcg gtgttaagtg cctatggtga tcagggcggc tttgtgctgg
    10141 ctccgcagga taacctcttt tgaatctgaa cgagtcgatg gctcgcgaat aatccgatta
    10201 cggctacgct tctaatgttc cccttgaatg gagtcgaaga atgcgtaatc ccacgctgtt
    10261 acaatgtttt cactggtatt acccggaagg cggtaagctc tggcctgaac tggccgagcg
    10321 cgccgacggt tttaatgata ttggtatcaa tatggtctgg ttgccgcccg cctataaagg
    10381 cgcatcgggc gggtattcgg tcggctacga ctcctatgat ttatttgatt taggcgagtt
    10441 tgatcagaaa ggcagcatcc ctactaaata tggcgataaa gcacaactgc tggccgccat
    10501 tgacgccctg aaacgtaatg acattgcggt gctgttggac gtggtagtca accacaaaat
    10561 gggcgcggat gaaaaagaag ctattcgcgt gcagcgtgta aatgctgatg accgtacgca
    10621 aattgacgaa gaaatcattg agtgtgaagg ctggacgcgt tacaccttcc ccgcccgtgc
    10681 cgggcaatac tcgcagttta tctgggattt caaatgtttt agcggtatcg accatatcga
    10741 aaaccctgat gaagatggca tatttaaaat tgttaacgac tacaccggcg aaggctggaa
    10801 cgatcaggtt gatgatgaat taggtaattt cgattatctg atgggcgaga atatcgattt
    10861 tcgcaatcat gccgtgacgg aagagattaa atactgggcg cgctgggtga tggaacaaac
    10921 gcaatgcgac ggttttcgtc ttgatgcggt caaacatatt ccggcgtggt tttataaaga
    10981 gtggatcgaa cacgtacagg aagttgcgcc aaagccgctg tttattgtgg cagagtactg
    11041 gtcgcatgaa gtcgataaac tgcaaacgta tattgatcag gtggaaggca aaaccatgct
    11101 gttcgatgcg ccgctgcaga tgaaattcca tgaagcatcg cgcatggggc gcgactacga
    11161 catgacgcag attttcacgg gtacattagt ggaagccgat cctttccacg ccgtgacgct
    11221 cgttgccaat cacgacaccc aaccattgca agccctcgaa gcaccggtcg aaccgtggtt
    11281 taaaccgctg gcgtatgctc taattttgtt gcgggaaaat ggcgttcctt cggtgttcta
    11341 tccggacctc tacggtgcgc attacgaaga tgtcggtggt gacgggcaaa cctatccgat
    11401 agatatgcca ataatcgaac agcttgatga gttaattctc gcccgtcagc gtttcgccca
    11461 cggtgtacag acgttatttt tcgaccatcc gaactgcatt gcctttagcc gcagtggcac
    11521 tgacgaatat cccggctgcg tggtggtcat gtcgaacggg gatgatggcg aaaaaaccat
    11581 tcatctggga gagaattacg gcaataaaac ctggcgtgat tttctgggga atcggcaaga
    11641 gagcgtagtg accgacgaaa acggcgaagc gaccttcttt tgcaacggcg gcagcgtcag
    11701 cgtgtgggtt atcgaagagg tgatttaaat tcatccccgg cggcaagccg gggagatttc
    11761 attacggcag tggcgtcggt aacggcgctt tatccagcgc ggcggcacac tctgccgtcg
    11821 ggcgatccac acgctccatc gtcataccat cgtactcaat ggtgttaccg tcacgctcaa
    11881 cctcgtacag ctcacgtttt acggtaacgt tagtgagatc atcagacatc aatgtcaact
    11941 tgcccggcac ggcaatcacc cgctgccact gacggcaatc cagggtatcg ccctctttag
    12001 tgacaatcag gctgcctatc gcttccggac tgaccagcgc acgttgcggc cctttggtct
    12061 gccagtagcc cgccagccag tccggcgcgg gggttttcac gacattgtta tagttttcga
    12121 cttcggcaca gcccgccagc aacatgagcg cgcttgcaat tgcgagtttt ttcatcattc
    12181 gtcctgcatg agaagaaaag aggattgtgg cattaaagcc ctgatgtcgc cagcctttcc
    12241 aggttcagag agaaatttga tctaactatt atttttgtga attattaccc cgaaaataaa
    12301 tttgcatgat gtatgattcg cgattcttgt ttcaaacctt ctgagttcag aggctacatt
    12361 catgtcatgg cagcaattca aacacgcctg gttgattaaa ttctgggcac ccatccctgc
    12421 ggtcatcgcg gcgggtattc tctccactta ctattttggc attactggca ccttttgggc
    12481 tgtcacgggt gaatttaccc gttggggcgg ccagctcctg cagctgtttg gcgtccatgc
    12541 tgaagagtgg ggttatttta aaattatcca tctggaagga tcgccattaa cccgcatcga
    12601 cgggatgatg atcctcggca tgtttggcgg ctgctttgcc gcagcgctgt gggccaacaa
    12661 tgtcaaactg cgcatgccac gcagccgtat ccgcattatg caggccatta ttggcggtat
    12721 tatcgctggt tttggcgcgc gtctggcaat gggctgtaac ctggcggcgt tctttaccgg
    12781 tattcctcag ttctcgctgc atgcctggtt ctttgccatc gccactgcca ttggttcatg
    12841 gtttggcgca cgctttaccc tgctgcccat cttccgtatt cccgtgaaaa tgcagaaagt
    12901 ttctgccgcc tcaccgctga cgcaaaaacc ggatcaggcg cggcgtcgtt ttcgtctcgg
    12961 gatgctggtc tttttcggta tgctgggctg ggcgctgctc acggcgatga accaaccaaa
    13021 actggggctg gcaatgctgt ttggcgtcgg ctttggttta ctgattgaac gtgcgcaaat
    13081 ctgctttact tcggcgttcc gcgatatgtg gatcaccgga cgtacccata tggcgaaagc
    13141 aatcattatc ggtatggcgg tgagtgccat cgggatcttc agttacgtac agttaggcgt
    13201 tgaacccaaa atcatgtggg cgggaccaaa cgcggtaatt ggtggtttac tgtttggttt
    13261 tggcatcgtg ctggcaggcg gctgcgaaac cggctggatg taccgcgcgg tagaaggcca
    13321 ggtgcactac tggtgggtcg gtctgggcaa tgtgatcggc tcgacgattc tggcgtatta
    13381 ctgggatgat ttcgctccgg cgctggccac cgactgggac aaaatcaacc tgctgaaaac
    13441 ctttggcccg atgggtggcc tgctggtgac atatttgctg ttgtttactg cgctgatgtt
    13501 gattatcggc tgggaaaaac gcttcttccg ccgtgcggca ccgcagactg ctaaggagat
    13561 cgcatgaaaa atatcgttcc tgattaccgc cttgatatgg tgggtgaacc ctgcccttat
    13621 ccggcagtcg caactcttga ggcgatgccg cagttaaaaa aaggggaaat cctggaagtg
    13681 gtgagcgact gtccacagtc gatcaataat attccactgg atgcgcgtaa tcacggctat
    13741 acggtgctgg atattcagca agacgggccg accattcgtt atttaattca gaagtaatcc
    13801 ctcattcccg ctgtggccca cagcgggaac atcgttgcct ctgacacata gattttaccc
    13861 gccccctctc ctattctttt cagtatgatc tcaggaggtt tctatgtgtg gacgctttgc
    13921 ccaatcccaa acgcgtgaag attaccttgc gcttctcgcg gaagatattg aacgcgatat
    13981 tccctacgat cccgaaccca ttggcagata caacgtcgcg ccgggaacca aagttcttct
    14041 gctcagtgaa cgtgatgaac accttcatct ggatccggtt ttctggggat atgctcccgg
    14101 atggtgggat aaaccgccgc tgattaacgc ccgcgtagaa actgcggcca ccagtcgtat
    14161 gtttaaaccg ctctggcaac atggtcgggc aatctgtttt gccgatggct ggtttgagtg
    14221 gaaaaaagaa ggcgacaaaa aacagcctta ttttatctat cgcgctgatg gacaacctgt
    14281 ttttatggcc gcgataggta gcacaccgtt tgagcgcggt gacgaagccg aaggattttt
    14341 aattgtcact gcagcagcag accagggtct ggtagatatt catgaccgcc gcccgctggt
    14401 tctgtcgcca gaagctgcac aggaatggat gcggcaagag attggcggta aagaagcatc
    14461 agaaattgct accaacggct gtgtgccagc aaaccagttc acctggcacc cggtatcgcg
    14521 cgcggtgggt aatatcaaaa accagggagc ggagttaatt caaccggttt gataagccag
    14581 gagactattg atcaataata ctgcgtcata agaaatctct attagacaat agtagacgct
    14641 ccttccacat gcgccacaag aaacctctat tccagtgaca caattacgcc tgattatttc
    14701 cctagcccgg aatatgaata aaaacatcaa caaagattaa attattcaaa cgttactgtt
    14761 gcgtctgcgg tcgtagcaac atccgacgtg gtgatgagtg ttaaaagaga cactctgatg
    14821 ttcagagggg ttatcgcgtt tacggatcgt ggatggcaga aaactttcag gatcaggtat
    14881 ccattttaaa ccagaaattg agtgagtttg ccccatccat gccccaagcg gtgagattgg
    14941 aagtaattaa caacaggtta caaaacctac acctgcatgg acatcacttc ctgatacgcc
    15001 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
    15061 tcggtcatca catcgtttaa cgccacgccg ggttcaccga gggtgaattt ttctgcctgc
    15121 gtgcgggcag ctgtttgtgt atcgctaatg cgatcgagcg cggcgtgcag ctgcccggca
    15181 aaactaatgg tcggttgcgg cagtgaatcc tgcgcacgcg cactcatcgc cgtagcctgt
    15241 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc ctggataatt
    15301 tctggtagca aagcctacca gtaagtcaat aagataaagg cgctaaataa caacaaaaaa
    15361 acgggtttat tggcggatag aaaaaaacga aagcacaaat aatggcagcg tcaatttttc
    15421 gagtttgctg acccgggagt gagtcttgtt ccactttgcc aataacgccg tccataatca
    15481 gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa aatctcttga gtggcttaat
    15541 cgcctgcgtg cgaatccgaa aattccattg attgttgccg gttccgcggc agtggcggtc
    15601 atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc gcacattatt cagcaatctt
    15661 tccgatcagg atggtggcgc aattgtcagc caactgacgc aaatgaatat tccttaccgc
    15721 ttcagcgaag ccagcggcgc tattgaagtt ccggcagata aagttcacga actgcgtctg
    15781 cgcctggcac aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact actcgatcag
    15841 gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt atcagcgggc gctggaaggc
    15901 gagctttctc gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt acatctggca
    15961 atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta
    16021 aatctgttac ccggccgcgc actcgatgaa gggcaaatta gcgccattgt gcatctggtt
    16081 tccagcgccg ttgctggtct gccgccggga aacgtcacgc tggtggatca gggcggacat
    16141 ctgttaaccc agtccaatac cagcgggcgc gatcttaatg atgctcagtt gaaatatgcc
    16201 agcgatgtcg aaggccgtat tcagcggcgt attgaagcaa tcctgtcgcc tattgttggt
    16261 aacggtaata ttcacgccca ggtcacggcg cagctggact tcgccagtaa agaacaaacg
    16321 gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg cgcttcgttc acgccagctt
    16381 aatgagagcg agcaaagcgg ttccggttat ccgggcggcg taccgggggc gttgtcgaat
    16441 caaccggcac ctgcgaataa cgcgccaatc agcacgcctc cggcaaatca aaataaccgc
    16501 cagcagcagg cgagcaccac cagcaatagc gggccgcgta gcacacagcg gaatgaaacc
    16561 agtaactacg aagtcgatcg caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa
    16621 cgtctgtcag tcgcggtagt ggtgaattac aaaaccttgc cagacggtaa accattgcct
    16681 ctcagcaacg aacagatgaa gcaaattgaa gatctgaccc gcgaggcgat gggcttttct
    16741 gaaaaacgcg gtgactcgct caatgtcgtt aactcgccgt tcaatagcag tgacgaaagc
    16801 ggcggagaac tgccgttctg gcaacagcaa gcgtttatcg atcagttgct ggctgccggt
    16861 cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc ggaaagcggt acgcccgcag
    16921 ttaacacgtc gcgccgaggc gatgaaagct gtacagcaac aggcgcaggc ccgtgaggaa
    16981 gtggaagatg cggtggaagt ccgcctgagc aaagacgaac aactccaaca acgacgcgct
    17041 aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat
    17101 ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac
    17161 aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt
    17221 gttcaagcac ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac
    17281 gcagatcacc aacaagcagc taaccgatgt gctggcggag tttgagcaag aagctgaaca
    17341 gtttgccgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct
    17401 gggtgaggaa cgtgccgcca gcctgctgga agatattctc gaaactcgcg ataccgccag
    17461 cggtattgaa acgctcaact ttatggagcc gcagagtgcc gccgatctga ttcgcgatga
    17521 gcatccgcaa attatcgcca ccattctggt gcatctgaag cgcgcccagg ccgccgatat
    17581 tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg
    17641 cggcgtgcag ccagccgcgc tggcggaact gaccgaagta ctgaatggct tgctcgacgg
    17701 tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct
    17761 gatgaaaact cagcaggaag aagccgttat taccgccgtg cgcgaattcg acggcgagct
    17821 ggcgcaaaaa attatcgacg agatgttcct gttcgaaaat ctggtggatg tcgacgatcg
    17881 cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgttgatcg cactgaaagg
    17941 ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat
    18001 tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg aaaacgaaca
    18061 gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact ggcgagatgg taattggcag
    18121 cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg gacgatctcg
    18181 cgccaccaca ggcagagttt gtgcccatgg tcgagccgga agaaaccatc attgaagagg
    18241 ccgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccat gagcaaggtt
    18301 atcaggcggg tattgccgaa ggtcgccagc aaggtcataa gcagggctat caggaaggac
    18361 tggcccaggg gctggagcaa ggtctggcag aggcgaagtc tcaacaagcg ccaattcatg
    18421 cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcactt gatagtgtga
    18481 ttgcttcgcg cctgatgcag atggcgctgg aggcggcacg tcaggtcatc ggtcagacgc
    18541 caacagtgga taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt
    18601 tattcagcgg caaaccgcaa ctacgcgtgc accccgatga tctgcaacgt gtggatgata
    18661 tgctcggcgc aaccttaagt ttgcatggct ggcgcttgcg gggcgatccc acactccatc
    18721 ctggcggctg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct
    18781 ggcaagaact ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg
    18841 ctggctaacc acgctggata actttgaagc caaaatggcg cagttgcctg cggtacgtcg
    18901 ctacgggcga ttaactcgcg ctaccgggct ggtgctggaa gccaccggat tacaattgcc
    18961 gctcggcgca acctgtgtca ttgagcgcca gaacggcagc gaaacgcacg aagtagaaag
    19021 cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg ccgctggagg aagtcgaagg
    19081 tgtcctgccc ggcgcgcgtg tttatgccaa aaacatttcg gcagaagggc tgcaaagcgg
    19141 caagcagttg ccgctcggtc cggcgttatt aggtcgtgtt ctggacggca gcggtaaacc
    19201 gctcgatggc ctcccctccc ccgatacgac ggaaaccggt gcgctgatta ccccaccatt
    19261 taacccgttg caacgtacac cgattgaaca tgtgctggac accggcgtgc gcccaatcaa
    19321 tgccctgctt accgttgggc gtgggcagcg tatggggctg tttgccgggt ccggcgttgg
    19381 taaaagtgtg ctgctgggga tgatggcacg ttacacccgc gccgatgtca ttgtcgtggg
    19441 tttgattggt gaacgcggac gcgaagtaaa agattttatt gagaatatcc tcggtgccga
    19501 agggcgtgca cgctccgtgg tgattgccgc tccggcggat gtctctccgc ttctgcgaat
    19561 gcaaggtgcc gcctatgcca cgcgaattgc cgaagatttt cgcgatcgtg gtcagcatgt
    19621 gttgctgatt atggactccc tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc
    19681 gattggcgaa ccacctgcca ctaaaggtta tccaccgtcg gtgtttgcca aattaccggc
    19741 actggtcgag cgtgccggaa atggaattag cggcggcggc tcgattaccg cgttttatac
    19801 cgtgctcacc gaaggcgatg accagcagga ccccattgcc gactctgcgc gggcaatcct
    19861 cgacggtcac attgtgctgt ctcgccgact ggcggaagcc gggcactatc cggctatcga
    19921 tattgaagcg tcgatcagcc gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg
    19981 agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt
    20041 cggcgcgtat gccaaaggca gcgatccgat gctcgacaaa gccatcgccc tgtggccgca
    20101 gctggagggc tatttgcaac aaggcatttt tgaacgcgcg gactgggaag cgtctctcca
    20161 ggggctggag cgtattttcc cgacagtgtc ataacccagg agataacggc agatggcaga
    20221 acatggcgcg ctggcgaccc tgaaagatct ggcagaaaaa gaggtagagg atgccgcgcg
    20281 cctgctgggt gaaatgcgtc gcgggtgtca gcaggcggaa gaacagctca aaatgctgat
    20341 cgattatcag aatgaatatc gcaataacct caacagcgat atgagtgccg ggatgaccag
    20401 caaccgctgg atcaactatc agcagtttat ccagacgctg gaaaaagcca ttactcagca
    20461 tcgccagcaa cttaatcagt ggacgcagaa agttgacatt gccctgaaca gctggcgaga
    20521 aaagaaacaa cgtttgcagg cctggcagac actgcaggaa cggcaatcca cggcggcact
    20581 gcttgcagaa aaccgcctcg atcagaaaaa gatggatgag ttcgcccagc gcgccgccat
    20641 gaggaaacct gaatgattcg cttagcgccc ttaattaccg ccgacgttga taacgccaca
    20701 ttgcctggcg gcaaagccag cgatgctgca caagattttc tcgcgttgtt gagcgaagca
    20761 ttagcaggcg agacaactac cgacaaagcg gccccccagt tgctggtggc aacagataag
    20821 cccacgacaa aaggcgagcc gctggtcagc gagattcttg ccgatgcgca acaagcggat
    20881 ttactgatcc ctgtggatga aacaccgcct gtcatcaacg acgaacaatc cacatcaaca
    20941 ccattaacca ccgctcaaac gatgacgttg gctgcggtgg ctgacaacaa tacggcaaaa
    21001 gacgaaaaag cggatgatct gaatgaagac gtcaccgcaa gcctgagcgc cctttttgcg
    21061 atgttgccgg gttttgacaa tacgcccaaa gtgactgatg tgccgtcaac cgtgttaccg
    21121 gcagagaaac caacgctctt cacaaaactg acttctgcgc aactcacaac agcacagcct
    21181 gatgatgccc ccggcacgcc agctcagcca ttaacaccgc tggtagcaga agcccagagt
    21241 aaagcggaag tcatcagcac accttcaccg gtgaccgctg ccgccagccc gctaatcact
    21301 ccacaccaga cacagccact gcccaccgtc gccgcgcctg tgttgagtgc accgctgggt
    21361 tctcacgaat ggcaacaatc attaagccag catatttcgc tgttcacccg ccaggggcaa
    21421 caaagtgcag agttgcgtct gcacccgcag gatttaggtg aagtgcaaat ctccctcaaa
    21481 gtggatgata accaggcaca aatccagatg gtttcaccgc atcagcatgt acgcgccgcg
    21541 ctggaagcag cgcttccggt actgcgtacg cagctggccg aaagtggcat tcagttaggg
    21601 caaagcaaca tcagtggcga aagctttagt ggtcagcagc aggccgcttc ccaacaacag
    21661 caaagccaac gcacagtaaa ccatgaacct ctggcggggg aagacgacga tacgcttctg
    21721 gttcccgtct ctatacaagg gcgcgtaaca ggcaacagcg gcgttgatat tttcgcctaa
    21781 cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt tgttgcgctc aagacgcagg
    21841 ataattagcc gataagcagt agcgacacag gaagaccgca acacatgact gattacgcga
    21901 caagcaagaa aagcaagcga tcgctttgga tcccgattct ggtattcatt accctcgcgg
    21961 cctgtgccag cgcaggttac agctactggc attcgcatca ggttgccgct gacgacaaag
    22021 cgcagcaacg cgtcgtaccc tccccggtct tctacgcgct ggataccttc acggtcaatc
    22081 tgggcgatgc ggatcgcgtg ctttatatcg gcataaccct gcgcctgaaa gatgaagcta
    22141 cccgctcgcg gctgagtgag tatttgccgg aagtccgtag ccgtttgctg ttactgtttt
    22201 cgcgtcagga tgctgccgta ctggcgacag aagaaggcaa gaaaaacctg attgccgaga
    22261 ttaaaaccac gctttccacc ccgcttgttg ccgggcaacc gaaacaggat gtcaccgacg
    22321 tgctgtatac cgcttttatt ctgcgataac gacatgggcg atagtattct ttctcaagct
    22381 gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag acgaaccgac agccagtgtt
    22441 agcggcgaaa gtgacattcg tccgtacgat ccgaataccc aacgacgggt tgtgcgcgaa
    22501 cgtttgcagg cgctggaaat cattaatgag cgctttgccc gccattttcg tatggggctg
    22561 ttcaacctgc tgcgtcgtag cccggatata accgtcgggg ccatccgcat tcagccgtac
    22621 catgaatttg cccgcaacct gccggtgccg accaacctga accttatcca tctgaaaccg
    22681 ctgcgcggca cagggctggt ggtgttttca ccgagtctgg tgtttatcgc cgtggataac
    22741 ctgtttggcg gcgatggacg cttcccgacc aaagtggaag gtcgcgagtt tacccatacc
    22801 gaacagcgcg tcatcaaccg catgttaaag ctggcgcttg aaggctatag cgacgcctgg
    22861 aaggcgatta atccgctgga agttgagtac gtgcgttcgg aaatgcaggt gaaatttacc
    22921 aatatcacca cctcgccgaa cgacattgtg gttaacacgc cgttccatgt ggagattggc
    22981 aacctgaccg gcgaatttaa tatctgcctg cctttcagca tgatcgagcc gctacgggaa
    23041 ttgttggtta acccgccgct ggaaaactcg cgtaatgaag atcagaactg gcgcgataac
    23101 ctggtgcgcc aggtgcagca ttcacagctg gagctggtcg ccaactttgc cgatatctcg
    23161 ctacgcctgt cgcagatttt aaaactgaaa cccggtgacg tcctgccgat agaaaaaccc
    23221 gaccgcatca tcgcccatgt tgacggcgtc ccggtgctga ccagtcagta cggcactctc
    23281 aacggtcagt atgcgttacg gatagaacat ttgattaacc cgattttaaa ttctctgaac
    23341 gaggaacagc ccaaatgagt gacatgaata atccggccga tgacaacaac ggcgcaatgg
    23401 acgatctgtg ggctgaagct ttgagcgaac aaaaatcaac cagcagcaaa agcgctgccg
    23461 acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg aacgttgcag gatatcgacc
    23521 tgattatgga tattccggtc aagctgaccg tcgagctggg ccgtacgcgg atgaccatca
    23581 aagagctgtt gcgtctgacg caagggtccg tcgtggcgct ggacggtctg gcgggcgaac
    23641 cactggatat tctgatcaac ggttatttaa ttgcccaggg cgaagtggtg gtcgttgccg
    23701 ataaatatgg cgtgcggatc accgatatca ttactccgtc tgagcgaatg cgccgcctga
    23761 gccgttagtg atgaataacc acgctactgt gcaatcttcc gcgccggttt ctgctgcgcc
    23821 actgctgcag gtgagcggcg cactcatcgc cattattgcc ctgatcctcg ctgctgcctg
    23881 gctggtaaaa cggttgggat ttgtccctaa acgcactggc gttaacggtc tgaaaattag
    23941 cgccagtgct tcgctgggcg cgcgtgaacg ggttgtggtg gtcgatgtgg aagatgcacg
    24001 gctggtgctc ggcgttaccg cagggcaaat caatctgctg cataaacttc ccccttcagc
    24061 accaacggaa gagataccgc agcccgattt tcagtcggtt atgaaaaact tgcttaagcg
    24121 tagcgggaga tcctgatgcg tcgtttgttg tctgtcgcac ctgtccttct ctggctggtt
    24181 acgcccctcg ccttcgcgca actgccgggt atcaccagcc agccgctgcc tggcggtgga
    24241 caaagctggt cgctcccggt gcaaacgctg gtgttcatca cctcgttgac gtttattccg
    24301 gcaattttac tgatgatgac cagtttcacc cgcatcatca ttgtttttgg tttattgcgt
    24361 aacgcgttgg gaacaccctc cgcgccacct aaccaggtat tgctgggact ggcgctgttt
    24421 ttgacctttt ttattatgtc gccggtgatc gataaaattt atgtcgatgc gtaccagcca
    24481 ttcagcgaag agaaaatctc aatgcaggag gcgctggaaa aaggggcgca gccgctgcgt
    24541 gagtttatgc tgcgtcagac ccgcgaggcg gatttagggt tgtttgccag actggcgaat
    24601 actggcccgt tgcagggacc tgaagctgtg ccgatgcgca ttttgctccc ggcctacgtg
    24661 accagcgagt tgaaaaccgc atttcagata ggcttcacga ttttcattcc ttttttgatt
    24721 atcgacctgg tgattgccag cgtgttgatg gcattgggga tgatgatggt tcccccagcc
    24781 acaattgctc tgccctttaa actgatgctg tttgtactgg tggatggctg gcaattgctg
    24841 gtcggttcgc tggcgcagag cttttacagc tagagaggca aaatgacacc tgaatcggtc
    24901 atgatgatgg ggactgaagc gatgaaagtc gcgctggcac tggctgcccc actgttgctg
    24961 gtggcgctgg tcacgggtct tatcatcagt attttgcagg ccgccaccca gattaacgaa
    25021 atgacgctgt cgtttattcc gaaaatcatc gccgtattta tcgccattat tattgccgga
    25081 ccgtggatgc tcaatctgtt gctggattac gtccgcacct tgttcactaa cctgccgtat
    25141 atcatcgggt aggccgtaca atgatgcagg taacaagcga tcaatggctt tcctggttaa
    25201 gcctgtactt ctggccgtta ctgcgcgtgc tggcgctcat ttctaccgca ccaattctga
    25261 gcgaacgcag cgtgccgaaa cgggtaaaac tgggtctggc aatgatgatc acgttcgcta
    25321 ttgccccatc attacctgcc aacgatgttc ctgttttttc gttctttgct ctgtggctgg
    25381 ccgtgcagca gatcctgatc ggcattgcgc ttggttttac catgcaattt gccttttccg
    25441 ctgtgcgaac cgctggtgaa attatcggtc tgcaaatggg gctgtcattt gcgacgtttg
    25501 tcgatccggc cagccatctc aatatgcccg ttttagcgcg tatcatggat atgctggcgt
    25561 tactgctgtt cctgacattt aacggtcatt tatggttgat ttcactgctg gccgatacct
    25621 ttcacaccct gccaattggt ggcgaaccgt tgaacagcaa tgcgtttctg gcactcacca
    25681 aagcagggag tttgattttc cttaacgggc tgatgctggc attaccgctc attactctgc
    25741 tgctgaccct gaatctggca ttaggcttac ttaatcgtat ggccccgcaa ttatccattt
    25801 ttgttattgg atttccatta actctgactg tcggtatctc tttaatggcg gcattaatgc
    25861 cgttaattgc gcctttttgc gaacatttat tcagtgagat gtttaatttg ctggctgata
    25921 ttattagtga attgccatta atataattcc gtaacgttta tcatgttatc ctaaggatta
    25981 tccgaaaaat aatacctacg aacatcttcc aggatactcc tgcggcgaaa tatttgtttt
    26041 aagctcactc acatatcgca acatttactt tactttaaga caattccagg caaattatac
    26101 aacactttac gggatagtaa gtccgcctga aaaatcgcga gagtggcgca ttaggtgacc
    26161 catgttgttc cgtttagtca tgatgaaata ttcaggtaag gggaattatc gttacgcatt
    26221 gagtgagggt atgccatgtc aacgattatt atggatttat gtagttacac ccgactaggt
    26281 ttaaccgggt atctgttgag tagaggggtt aaaaaaagag aaatcaacga cattgaaacc
    26341 gttgatgacc ttgccatagc ttgtgattca cagcgccctt cagtggtgtt tattaatgag
    26401 gactgtttca tccacgatgc ttctaacagt cagcgtatca agctcatcat taatcaacat
    26461 cccaatacgt tatttatcgt ttttatggca attgccaatg ttcattttga tgaatatcta
    26521 ttggtcagaa aaaatttatt gatcagttct aaatcgatta aaccggaatc tctcgacgat
    26581 atccttggcg atattctgaa aaaagagaca acgataacct cgtttttaaa tatgccgacg
    26641 ttatcattga gccgaaccga atcgagtatg ttgcgaatgt ggatggcagg tcagggaacc
    26701 attcaaatct ctgaccaaat gaatatcaaa gccaagaccg tttcatcgca taaaggtaat
    26761 attaaacgta agatcaaaac gcataataaa caggttatct accatgtcgt ccgactgacg
    26821 gataatgtga ctaatggtat ttttgtcaac atgcgctaac acattctgac tggtggtttc
    26881 ccaccagtca ggccgaatta gattactctg ctttctccac aaagataccg tcctgatgcc
    26941 ctgcttcatt aaagaaccag atgccgagcg ggtagtcttc cagcgaaacc aggtacattg
    27001 tgccttcact aaactcctca actgccagta ccacgccagg acgacgcgga ccgccatccg
    27061 ttttgactgt tacccgatca ttcaccttca tcgttttcct cctgtggctt tgtgccagtg
    27121 tagaacaatt tcgtttttct ggcagcgcca ggcgcgcgcg agtgctgatt ttctcgacgg
    27181 tctatactta agagatgcca ggcggactta acgactggcc gcaacaacag agtaacggtt
    27241 gcgaggaaag atgatgaaaa ccgcaaaaga gtacagcgat accgcaaaac gtgaggtcag
    27301 cgtcgatgtc gatgccctgc tggcggcgat caatgaaatt agcgaaagcg aagttcatcg
    27361 cagccagaac gattctgaac acgttagcgt cgatggacgt gaatatcata catggcgtga
    27421 attggcggat gccttcgaac tggatattca tgacttcagc gtctctgaag tgaatcgttg
    27481 aatgcacaat aaaaaaatcc cgaccctgag ggggtcggga tgaaacttgc ttaagcaaga
    27541 agcacttaaa aaattcgtta caccaggaaa tctgatgtgt tcatcacctt atccgcaatt
    27601 tttttcgctg acaagaaaat attcgtgata tgaatgatta acgctactta tgaatagaaa
    27661 tgtgacgcgc gtcacccctt ggtggctctg gcgtcaacgg gatcgctccc gaaaaaatca
    27721 tctttgataa tttgtgctgt tttgagaatc atcctggtaa ggggagtact gcgcaaccat
    27781 gctttcaatt caacaaccac tactggtttt tagcgatctt gatggcaccc tgctggacag
    27841 tcatagttat gactggcaac cagctgcccc ctggctcagc cgtttacgcg aagcgaatgt
    27901 tcccgtcatt ctctgtagca gtaagacatc agccgaaatg ctgtacttgc aaaaaacgtt
    27961 ggggctacaa ggtttaccgc tgattgccga aaatggcgca gtgatccagc ttgctgagca
    28021 atggcaggat atagacggtt ttccacgcat catctcaggt attagccatg gcgaaatcag
    28081 ccaggtttta aatacgctac gcgagaaaga acattttaaa ttcacaactt ttgatgatgt
    28141 cgacgatgca accatcgccg aatggacggg attaagccgt agccaggcgg cgctgacgca
    28201 gctacatgag gcgtcggtaa cgctaatctg gcgcgacagt gacgagcgta tggcacaatt
    28261 taccgctcgt ctgaacgaac tgggcttgca gtttatgcag ggtgcgcgct tctggcacgt
    28321 cctggatgcc tctgccggaa aagatcaggc tgccaactgg attatcgcga cctatcaaca
    28381 atcgtcaggc aaacgcccaa ccacacttgg cctgggcgat gggccaaacg atgcgccctt
    28441 actggaggta atggattacg cggtgattgt gaaagggtta aaccgtgaag gggtgcatct
    28501 gcatgatgag gatccggccc gcgtctggcg aacgcagcgt gaaggaccgg aaggctggcg
    28561 tgaagggctg gaccattttt tctccgccca ttaagcgtta tcgctcgcga atacccgatt
    28621 gcgcccggcc tgtttagcga gataaagtcg acggtcggcc agtgactgga gttgttcaaa
    28681 atcataatca ccggtttcct cgctgctgct tacccccagc gaggcactga tgcgtatcgt
    28741 cgtactcttg gcgatcaaca tctctttttc atttagcttc aggcgaatac gttctgcaac
    28801 ctccgcagcc tgcgtcaggc tcgcgcctgg cagaatcaca caaaactcct caccaccgac
    28861 ccgcccggca acatcctgcg ctcgcaagga actgctaatt aatccggcag catgagaaag
    28921 aacacggtcg cccgcctgat gaccaaagcg gtcattaatc gctttaaaat ggtcaaggtc
    28981 gacctgaatg acagaaaaag gatgttggtg cgtctgacac aatttagcga gcggacgggc
    29041 tttttcgaac agtgcgccac ggttatataa acgcgttaag gtgtcgtgcc acgcctgcca
    29101 ctgcaacgag ctttgcaaaa catacatgtt gctgaccatc cggcgaatca cataccagga
    29161 gatgagtaac atggtggtaa agagcgccca cagcagggtt aatgcaatgc tgatactgcc
    29221 gaaatcgccg cgcacgcctt cgcttagcgt atggacgcgc accagcacac cgtcgaaatg
    29281 gtccagacgt tcccagctaa catagcgact gttcatacga atgccgcccc gcgtgtcatg
    29341 ttccattgcc tgcgccagca aggccagttc acgaggatca aaaatattcc ctgttggatg
    29401 gtcaggattg gaagaggtca ataatctcag cttactgtca tagagctgat actcaccatc
    29461 gaggttttta tcgatggcgt ttcttaaaaa ttgctgcatg gtacgcacgg gaatactcat
    29521 ccccagcacg ccataccagt agttttcact gtctaccgga acactgacgg taacctgcgg
    29581 ttcagtattg ctggcgtgtt ccggttgcga agtgaaccag cgtaccgcgc ggtgacgatt
    29641 ttctcgttgc gaatggccga caaaccaggg ttgggtgaca tagccgtaat aacgcgttgg
    29701 tacattgcgc gtaaacaagg tcggctgcgt cgaaacgtaa aatccggcac gcgagacata
    29761 catcgcctgt tcaaccatcg acgaggagtt gtgtgccagt cgcagcaagt aaccaacttc
    29821 cagtgcagcg gtaatttcat tgtcgaggct ttcattttcg cgagacagga gattcccctc
    29881 gctgactaat gcatccgaca caccattgac tggcagggtg cgtcgtcggt tgagttcgat
    29941 ttgccaggcg tgctcgtcgc gatgctgttc gaactcggtt acggcattac gcagagaggt
    30001 gaaatccagt ggcgctacga gagcttcgcg catgccatta cgcaaaaaga tcagtttgtc
    30061 gacgttatac tgcaaatgct tatcgagcgc gttggctacg ttttccagat gattacgctg
    30121 gctggagata taggcatctt ccagcaccac cacttcacgc caggtgagca aggtggaaaa
    30181 aagcaatacc acgataaagc agagattaac gacatgacca ggccccaggc ggcgtgcgag
    30241 ttttttcaac cagctctggt tttccatttt tgtctcgtgc tgcaccctga cgactcccaa
    30301 cctgcttttt tatgattctg gcataacctg gcgatagcgt accgcaaaac agcgataatt
    30361 ttataatggt ggcatgataa gacgcgacag acatcgatag ttttataacg taggcatgat
    30421 aagacgcgat agcgtcgcat caggcaattc agtgcagaca ctggcacacc cgaccggatg
    30481 cgacgtgagc gccacatccg gtcgggtgtt aatgataccc tttcaaactt cttccgcaga
    30541 acgactcccc tcccccggaa caagctcggt ttcatgaaaa gcttcccgct tgaccccgta
    30601 accgtcatac caacgacact caaccatacc gctggagtat ccagtgacaa tcatccgcgg
    30661 gccgccctct ttaaccgtaa cttcctcact aaccataaag ctcatactcg cctccttttt
    30721 tcgagtgaaa cttgttcacc ttagttgaag atggcgaatt ttgcataata gcctgtgcga
    30781 gtattaatgt gcctgaccgc gcattttcgc tacggctttc actcccagca ccactatgcc
    30841 gccgatgata aatccaagaa tcagatttaa aacagtcggt aatatcatcg ccaccactgc
    30901 actttgctgc ccggcgaaat gttcaatggc gtgatgcagc ggcgcaattc catgtaccac
    30961 aatcccgccg ccgacgagga acatcgccag cgtgcctaca attgataacg ctttcatcag
    31021 ccagggcgca atgatcaata accctttacc taatgcctgc atcagcgcgc tggatttttc
    31081 cgccagccaa taccccaggt catcaatctt aacgataacc cctaccagac cgtagacgcc
    31141 cacggtcact accagcgcga tgcctgaaag caccagcacc tgattaagca acggcgcttc
    31201 ggccacaatc cccagcgtga tggcgacgat ttccgcagac aatataaaat cggtacgaat
    31261 cgcccctttt attttgtctt tttcaaactt cagcggatcc tgcgccgcca gcttctccag
    31321 acgctgctga ctctgcgccg gatcttcttt atgtttacgc gcctccagca tatgcagcac
    31381 tttctctacc ccttcaaagc agagaaacgc gccgccaatc atcaacagtg gcgtaatcgc
    31441 ccacgggata aacgcactga tgatcagcgc cagcggcacc agaatcactt tattgatcag
    31501 tgatcctttc gccacgcccc agaccacggg aagttcccgg ttggcgcgca cgcctgaaac
    31561 ttgttgcgca ttgagcgata agtcatcccc taatacaccg gcggttttct tcgccgccag
    31621 tttgcccatc acggagatat cgtccagcag tgtggcgata tcatcgagca acgtcagtaa
    31681 actactcccg gccaaaagca ttccctctca tttttattgt tgaagggtgt aagtatgaag
    31741 caaaaacggc agctctgata cttgcttcac aggtcaacat ttatttaaca atcaagtgca
    31801 attatatctc tcgccagcag aatgattttc acgtttacta tgaaccacct ttttatttca
    31861 gccgtgaggg attatgcgtt tccgccagtt gttaccgctt tttggcgcgc tgtttgcgtt
    31921 gtatatcatt tggggctcaa cctattttgt cattcggatt ggcgtggaaa gctggcctcc
    31981 gttaatgatg gcgggcgttc gattcctggc agccggtatt ttattgctgg catttttgct
    32041 actgcgcgga cacaaacttc ccccgctacg tccgctgctc aatgccgcgc tgattggcct
    32101 gttattgctg gctgtcggta atggcatggt gacggttgcc gaacatcaaa atgttccttc
    32161 cggcatcgcc gccgttgtgg ttgcaaccgt gcccctcttt accctgtgct tcagccgcct
    32221 gtttggcatt aaaacgcgca aactggaatg ggtgggtatc gccattgggc ttgccggaat
    32281 catcatgctc aatagcggtg gaaatttaag cggcaatccg tggggcgcga ttctgatttt
    32341 aatcggctcg attagctggg cgtttggctc ggtttatggc tcgcgcatta ccttacctgt
    32401 agggatgatg gcgggtgcga ttgagatgct ggcggctggc gtggtgttaa tgattgcgtc
    32461 gatgattgcg ggtgaaaaac tgacggcgct cccttccctt tcaggcttcc ttgcggtcgg
    32521 ctatctggcg ctgtttggtt cgattatcgc cattaacgct tatatgtatt taatccgcaa
    32581 cgtcagtccg gctctcgcca ccagctacgc ctacgttaac ccagtggtcg cggtcttgct
    32641 gggtacgggg ctgggtggcg aaacgctgtc gaagattgaa tggctggcgc tcggcgtaat
    32701 tgttttcgcg gtggtactgg tcacgttggg aaaatatctc ttcccggcca aacccgttgt
    32761 cgcgccagtt attccggatg catcaagcga gtaaatgaat cccctgcgtg tcgatctgcg
    32821 cgctggcacc ttcgccgcag atccactctt ccagacgctc ggtaagcgcc gcatccgtca
    32881 gcttctcgcg cccgcgtaac gcacattccc agacaatcaa tacgcgccaa ccgagttcct
    32941 gcaaatggct gatatcacga cgatcgcgct cgacattttt acctatcttc tccagccaaa
    33001 aatctgttcg cgtggcgggg actttaaaca ggtagcaatg gtgatggtgc cagaagcaac
    33061 cgtgggcaaa tatcacacag cgaaattcat caaggacaaa atccgggcgt ccgggcagac
    33121 tggcgtcctg aacgcgaaac gccaggccct gcccagttaa caaactggcg aggcgcttct
    33181 caatcgccgt atcacgcgtg gcaatcgcgc gcatattttt gctgcgggtg gccttatcgt
    33241 gaacgtcgac catgttgtgc ctcttgctga cgcaacgcca ccgcctgttt gatttttggc
    33301 tcaagcagtt ttgccaccgc ggcaaagacc ggcacgacca ccgagttacc gaactggcga
    33361 taagcctgag tgtccgaaac cggaatgcgg aatttcgctt ctcccggcgc ttcaaaaccc
    33421 attaagcgcg cacattcccg aggcgttaac cgacgtgggc gatgttgctg attcagcgga
    33481 tcgtcaaagt ctttctcacc cgttgccata tcccagccgc gatcgattaa aatttccgcg
    33541 ccatctttgt aataacgcgc agacagcgta cgcgtgacgc tttgcggatt gttcggataa
    33601 accattccat aaccgaagcc gttaccgcgc gcctgatgtt ttttcgcata tcgatagagg
    33661 tacttccaca gtaccggcgt caggatatat ttcgcctcga ccatcgggtc cagcagctgc
    33721 gccagcgtca ctcgctgtgc agggaaacat ttgctgatat cacgcagagt aaaatcggct
    33781 ttcagattca gatcgcgacg aaaacccacc agcacgatgc gttcacggtg ctgcggcaga
    33841 aaatgtttgc cgtcgatgat tttcggatcg tccggcccgt tatcttctgc atcagccacg
    33901 tcatagccca gttcgtccag cgtctgcatg atgatgcgga acgttttacc ctggtcgtga
    33961 cttttcaggt ttttgacgtt ttcgagcaca aacatcgccg gacgacgcgc gtcgataatg
    34021 cgcaccacat caaagaacag cgtgccctgg gtatcgcagg caaaaccgtg cgcccgcccg
    34081 agcgagttct ttttcgatac gccagccagc gaaaatggct gacaagggaa accggccagt
    34141 aaaacatcgt gttcaggaat gtgttgacga atatgttccg ccgccgcctc atcactcacg
    34201 ccttcttgat ggctgagggt gatgtcgcgg atatcctcat taaaatgatg cgtcgccggg
    34261 tcgcaataat ggttggcttt ataagtgcgt accgcatgtt tgttccattc gctggtaaac
    34321 acgcactgtc caccaatcga ttcaaaaccg cgacggatgc cgccaattcc ggcaaataga
    34381 tcgataaagc gaaacgcata atgcggatga tgtgccggtg gtttgggtaa caacgtttgc
    34441 agatgggcga actctttctc acttaaacgg tgccatgccg agtcattcgc cagcgcacgt
    34501 tttaaaattg ccgcgctcca gtgattctca cccacaccat taagctgcgc caccagcgtt
    34561 ttaacatcat aaatttgcag cagtttctcc agcattgcct gtgcggcatt cccggtgctg
    34621 tatgaatcgg ttactgatat attttcctgc atagatttaa ccggccatct gagatggccg
    34681 acaggttaac ataattactg ccgtttaggg ccacgcgctc agccagcagg tgaaaacgca
    34741 tctatcacct tgtgatcgat accttcattc tcccccgcca gttcggcacc gagcttcgcc
    34801 ataaactcga ctaaaaattg cgcgttgtgc tgtgccagct gcttgcctct tgcggtttgc
    34861 atggtttgcg gtaatttcag taactttgtc tggaaatgat ccagcgcgta gcgtttatca
    34921 tcaagcgggc gatgctgtgc gaacggatct tcgccatcaa acagcgccaa gcccaatgcc
    34981 cctgaaacgg caaatacgcg cgccaggcca atcgcccccc agggcttcca gccgatccgc
    35041 atcctggacg attttagctt ccgttgttaa gggggcgatt tgcgcgctga aactgtgagc
    35101 ggcaatggca tgacaaacgg cctcaatttt ctccgccgga aattgcacga actcttcggt
    35161 aatgctgcca acttactgat ttagtgtatg atggtgattt taaggtgctt gcgtggcttc
    35221 catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta acggcaaaag
    35281 cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc aactacagtt
    35341 cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg ccatgaatgg
    35401 cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg ttttacgtca
    35461 cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag tgatgtgatt
    35521 gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg tcagcgctgg
    35581 ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtatt cggtgaacgc
    35641 actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt cgtggtatgg
    35701 atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca cgttatcagc
    35761 aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca tctggcaagg
    35821 ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa ggtcatcggg
    35881 cattatctga acataaaaca ctatcagtaa gttggagtca ttaccccaaa accgtatcgc
    35941 ggtaaacata aaattctggt gatcgccgcg gacgaacgtt atttgccgac cgataacgga
    36001 aaactgttct cgaccggtaa ccatccgatt gaaacgttgc tgccgttgta tcatctccat
    36061 gctgcaggtt tcgaattcga agtggcgacc atttccggtc tgatgaccaa gtttgaatac
    36121 tgggctatgc cgcacaaaga tgaaaaagta atgccattct ttgagcagca taaatcgttg
    36181 ttccgcaatc cgaaaaaact cgcggatgtt gtcgtcagcc tcaacgcaga cagcgaatat
    36241 gcagcaatct ttgttcctgg tggtcatggc gcacttattg gtttaccaga aagccaggac
    36301 gtggctgctg ctttgcagtg ggcaatcaaa aatgaccgtt ttgttatctc cctttgccac
    36361 ggcccggcgg cttttctggc gcttcgccac ggcgataacc cactgaatgg ttattccatt
    36421 tgcgcattcc cagacgccgc agacaaacaa acgccagaga taggctatat gccgggtcat
    36481 ctcacctggt acttcggcga agagctgaag aaaatgggca tgaacattat taatgacgac
    36541 atcaccgggc gagtacataa ggaccgtaaa gttctcaccg gcgacagtcc tttcgcagcg
    36601 aatgcgttgg gtaaactggc ggcgcaggaa atgctggcag cttatgcggg ttaatccctg
    36661 ttacgtatga atgtaagcgc ataatcaatc gcagccacgt taatactcac gacgtggctg
    36721 cgttttttca tggttgattt atggcgtact gaagccctat gttaatttct ttgcggtaac
    36781 gtaattcgga acacattatg cttattaaaa tagtgatacg tagcacttcc cccatgcaat
    36841 tcggcaatcg ctttgactaa agaaagacca agtccctgac cttcggaatg acgcgaatta
    36901 tctccccgcc aaaatctacg gaagagtttt tcaggctcat taattttcgt tccaggactg
    36961 gcgatatcaa tattaagata gccgttggta tcaagaaaac tggttatatg aatgcgcgat
    37021 ttttctggtg aatatctaat ggcattaaca ataagattcg ataacattcg ttgcagtaaa
    37081 attttatccg caaagatttg ctgattgcac tcgaccttaa agcaaatctc tttctcgtct
    37141 gaaaggtatt caagataatc caacaaattt tcgacttcct tattgagaaa aagtgagtcc
    37201 agtttcacca aaacattgtt tttatctgcc cgggcaagaa acagtatgtt ctctgttaac
    37261 cgcgaaatat tttccagctc ttcaatgtta ccggcaattg tttttttata ttcagcgata
    37321 cttctggttt gactgagcgt aacctgattc tgacccagta atgcattaat gggcgttcta
    37381 agttcatgag cgagatcgtc agcaaactga cttagacgct caaaatcttt gactaaagca
    37441 tgatgcattt tattcaacgc ctgcccaaga ggttttagtt ctctcggtaa tgcgctaacc
    37501 tcaacaggct cccggctatc gttataattc agcgcttccg ttacaccact caactttttg
    37561 atctctcgta atcccgttct gattaacagc ggacttaata ctgagcaaag tacaatggcg
    37621 acaatgcaaa ttataatgct attaatttta tactgttcaa gcatgttatg tctggctgaa
    37681 gccaatttag ccacagtaac cgttaatgat ggcgaaactt catcaatatt aattcgtaaa
    37741 gcatctatct ctgtatcatt aataatgctt ctgtaaatgc cagctgcgct gattgtctca
    37801 gtagcaggta tgttatttaa catgtcatca ctgacattcg tccggttaac aattttgttg
    37861 atgctatcac catgaatgat caagatatcc tgactaacat ccatcatccg gttaaagtac
    37921 acaggtaacg tatctggatt taccccatca attaacaact gcttgatctg cgctgtccgg
    37981 ttaatgagtg ttgtatcatc gcgccatttc aactcacttg ccaagccatt atagagagtc
    38041 catacaattc cagcgccagc aacagacagc agcaatataa aaagcaaggt taaacggacg
    38101 gttatagata atcttttcat tattttttta ccgctacgaa tgaatagccc atcccccgga
    38161 ttgtggcgat tagcttttca ggaaaaggat catcaacttt tgcgcggagc ctgcgaatgg
    38221 cgacgtccac cgtattggta tcactatcaa agttgattcc ccaaatttca ctcgcaataa
    38281 ccgttctggg gataatctcg ccagctctgg aggccagtag ccaaagtaac tgaaactcct
    38341 tgcgcgtcag tgtaatactg atattgtccc tgctcacact ttgactaaca gagtccattc
    38401 ttaagccact gatttctaat gttgaattca aagcgtgatg ttgccttaat tgtgcccgaa
    38461 cccttgccag caactcagaa aatgaaaaag gttttaccag ataatcattt gccccactgt
    38521 ccagccctct gactctgtca tcgacagaat ctcttgcagt aaggcaaata acaggagttt
    38581 gctttgctgt tcttaacgtt tgtaagatct gccagccatc catacccgga agcataatat
    38641 ccagaatgat caatgcataa tcatccttaa gcgcaagata aagcccatct ctgccatcag
    38701 aaacggcatc aatgacataa cccgcttcgg aaagcccctg cgttacccac tcctgggtcc
    38761 tttgattatc ttcaataagt aaaatcttca tgaaaataat atgccataaa tcatatgtta
    38821 tagaaacagc ctggttcatt acaaaattgt aatgctgctg taaggttacc gtggacgctt
    38881 tttcgctatc ctcaaaactc attcacatga caaggatata aacatgttaa agcgttattt
    38941 agtactctcc gtagtaacgg ctgcattttc attaccttct ttggtctatg ctgcacaaca
    39001 aaacattctt agtgtgcaca ttttgaacca gcaaacagga aaacccgctg ccgacgtgac
    39061 agtcactctt gaaaagaaag cggacaacgg ctggttacaa cttaataccg ccaaaacaga
    39121 taaggatgga cgaattaagg cactgtggcc cgagcaaact gcaactacgg gcgattaccg
    39181 tgtcgtattt aaaaccgggg actatttcaa gaaacaaaat cttgaaagtt tcttccctga
    39241 gatccccgtt gaatttcata ttaataaagt gaacgagcat tatcatgtgc ctttattact
    39301 tagccaatat gggtattcaa cctatcgtgg cagttaatgc ataaatatgt aaaagcagat
    39361 ctctgctact gttagggagg acacttcctg acagattaac agtaaacggc tcttgctggc
    39421 taaagacaaa aaagtgtgat ggcttatgaa aaagaatcaa tttttaaaag aatcagatgt
    39481 tacggccgag tcggtattct ttatgacgcg tcggcaggtg ttaaaagcac tgggcatcag
    39541 cgcggctgca ctttctttgc ctcacgctgc gcatgccgat ctgcttagct ggtttaaagg
    39601 gaatgatcgc ccacccgccc ccgccggaaa accgttggag ttcagcaagc ctgccgcctg
    39661 gcaaaatgac ctgccactga cgccagcaga taaagtctcc ggttataaca acttctatga
    39721 attcgggctg gataaagccg atcccgccgc taatgctggt agcctgaaaa ccgatccatg
    39781 gacactgaaa atcagcggcg aagtggcaaa accattgacc ctcgatcacg atgatttaac
    39841 ccgtcgcttt ccgctggaag agcgtattta tcgtatgcgc tgcgtggaag catggtcgat
    39901 ggtggtgccg tggattggtt ttccgctgca caaattgctg gcgcttgccg aacccaccag
    39961 caatgcgaag tatgtcgctt tcgaaacaat ttatgcaccg gaacaaatgc caggccagca
    40021 ggaccgcttt atcggcggcg ggctgaaata tccttatgtc gaaggattgc gtctcgacga
    40081 agcaatgcat ccgctcacac tgatgaccgt gggtgtttat ggcaaggcgt taccgccaca
    40141 aaatggcgcg ccggtacgac tgattgtgcc gtggaaatat ggctttaaag ggattaaatc
    40201 gatagtcagt attaagctga cccgcgagcg tccgccaacc acctggaatc tggcagcgcc
    40261 tgacgaatac ggtttttacg ccaacgttaa tccgcatgtt gatcacccgc gctggtcaca
    40321 ggctaccgaa cgatttattg gttcaggcgg catcctcgat gttcagcgcc agccaacgct
    40381 actgtttaat ggttacgccg accaggtggc atcgctgtat cgtggcctgg atttgcggga
    40441 gaatttctga atgcgtctga cagcaaaaca ggtgacatgg ctgaaagtta gcctgcatct
    40501 tgccggattg ttgccgtttc tctggctggt ctgggcgatc aatcacggtg gactgggtgc
    40561 cgatccggtg aaagatattc agcattttac cggtcgcact gcactgaaat ttttgctggc
    40621 gaccttgtta atcacacctc tggcacgcta cgcaaaacag ccgttattga tacgcactcg
    40681 ccgcctgtta ggcttatggt gctttgcctg ggcgacactg catttaacca gttacgcatt
    40741 gctggagctg ggcgtgaata atctggcgtt attaggcaag gagttaatta cccggcctta
    40801 tttaacgtta ggaattatca gctgggttat tttgcttgct ttagcgttta cttcaaccca
    40861 ggcgatgcag cgaaaactcg gcaaacactg gcaacagttg cataatttcg tctatcttgt
    40921 cgcgatcctg gccccaatac attatctgtg gtcagtgaag attatttcac cgcagccgct
    40981 catctacgct gggctggctt tactgctttt agccttacgg tataagaagt tgcgttccct
    41041 ttttaaccgg ttacgcaagc aggttcacaa caaattatct gtataacttc atattgccga
    41101 caaagtacgc gttatccctg ccggatgcgg cgcgagcgcc ctatccggcc tacgcagacc
    41161 acatcaggtt atgtttcgtt tg
//
LOCUS       DAPGWD010000026        40344 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPGWD010000026 DAPGWD010000000
VERSION     DAPGWD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.026"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            325..411
                     /gene="leuL"
                     /locus_tag="RHX88_001332"
     CDS             325..411
                     /gene="leuL"
                     /locus_tag="RHX88_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDV2230044.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGSQH"
     gene            504..2075
                     /gene="leuA"
                     /locus_tag="RHX88_001333"
     CDS             504..2075
                     /gene="leuA"
                     /locus_tag="RHX88_001333"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDV2230045.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2075..3166
                     /gene="leuB"
                     /locus_tag="RHX88_001334"
     CDS             2075..3166
                     /gene="leuB"
                     /locus_tag="RHX88_001334"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDV2230046.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3169..4569
                     /gene="leuC"
                     /locus_tag="RHX88_001335"
     CDS             3169..4569
                     /gene="leuC"
                     /locus_tag="RHX88_001335"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDV2230047.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4580..5185
                     /gene="leuD"
                     /locus_tag="RHX88_001336"
     CDS             4580..5185
                     /gene="leuD"
                     /locus_tag="RHX88_001336"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDV2230048.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5234..6412)
                     /gene="setA"
                     /locus_tag="RHX88_001337"
     CDS             complement(5234..6412)
                     /gene="setA"
                     /locus_tag="RHX88_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HDV2230049.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6514..6645)
                     /gene="sgrT"
                     /locus_tag="RHX88_001338"
     CDS             complement(6514..6645)
                     /gene="sgrT"
                     /locus_tag="RHX88_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDV2230050.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6734..8389
                     /gene="sgrR"
                     /locus_tag="RHX88_001339"
     CDS             6734..8389
                     /gene="sgrR"
                     /locus_tag="RHX88_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDV2230051.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8423..8522
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8553..9536
                     /gene="thiB"
                     /locus_tag="RHX88_001340"
     CDS             8553..9536
                     /gene="thiB"
                     /locus_tag="RHX88_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDV2230052.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9512..11122
                     /gene="thiP"
                     /locus_tag="RHX88_001341"
     CDS             9512..11122
                     /gene="thiP"
                     /locus_tag="RHX88_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDV2230053.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11106..11804
                     /gene="thiQ"
                     /locus_tag="RHX88_001342"
     CDS             11106..11804
                     /gene="thiQ"
                     /locus_tag="RHX88_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDV2230054.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTMPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKARASALLGITG"
     gene            complement(11906..12670)
                     /locus_tag="RHX88_001343"
     CDS             complement(11906..12670)
                     /locus_tag="RHX88_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2230055.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12756..13634)
                     /gene="araC"
                     /locus_tag="RHX88_001344"
     CDS             complement(12756..13634)
                     /gene="araC"
                     /locus_tag="RHX88_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDV2230056.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13973..15673
                     /gene="araB"
                     /locus_tag="RHX88_001345"
     CDS             13973..15673
                     /gene="araB"
                     /locus_tag="RHX88_001345"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDV2230057.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGSLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15684..17186
                     /gene="araA"
                     /locus_tag="RHX88_001346"
     CDS             15684..17186
                     /gene="araA"
                     /locus_tag="RHX88_001346"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDV2230058.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17355..18050
                     /gene="araD"
                     /locus_tag="RHX88_001347"
     CDS             17355..18050
                     /gene="araD"
                     /locus_tag="RHX88_001347"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV2230059.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18125..20476
                     /gene="polB"
                     /locus_tag="RHX88_001348"
     CDS             18125..20476
                     /gene="polB"
                     /locus_tag="RHX88_001348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDV2230060.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20641..23547
                     /gene="rapA"
                     /locus_tag="RHX88_001349"
     CDS             20641..23547
                     /gene="rapA"
                     /locus_tag="RHX88_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDV2230061.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQIMESLDQAGWRLDALRLIVVTHQ"
     gene            23559..24218
                     /gene="rluA"
                     /locus_tag="RHX88_001350"
     CDS             23559..24218
                     /gene="rluA"
                     /locus_tag="RHX88_001350"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDV2230062.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24335..25150)
                     /gene="djlA"
                     /locus_tag="RHX88_001351"
     CDS             complement(24335..25150)
                     /gene="djlA"
                     /locus_tag="RHX88_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDV2230063.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25405..27759
                     /gene="lptD"
                     /locus_tag="RHX88_001352"
     CDS             25405..27759
                     /gene="lptD"
                     /locus_tag="RHX88_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDV2230064.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27812..29098
                     /gene="surA"
                     /locus_tag="RHX88_001353"
     CDS             27812..29098
                     /gene="surA"
                     /locus_tag="RHX88_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDV2230065.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29098..30087
                     /gene="pdxA"
                     /locus_tag="RHX88_001354"
     CDS             29098..30087
                     /gene="pdxA"
                     /locus_tag="RHX88_001354"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDV2230066.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30084..30905
                     /gene="rsmA"
                     /locus_tag="RHX88_001355"
     CDS             30084..30905
                     /gene="rsmA"
                     /locus_tag="RHX88_001355"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDV2230067.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSAEVLTGMGIDPSMRAENISVAQYCQMANYLAENAPLQES"
     gene            30908..31285
                     /gene="apaG"
                     /locus_tag="RHX88_001356"
     CDS             30908..31285
                     /gene="apaG"
                     /locus_tag="RHX88_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDV2230068.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31292..32134
                     /gene="apaH"
                     /locus_tag="RHX88_001357"
     CDS             31292..32134
                     /gene="apaH"
                     /locus_tag="RHX88_001357"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDV2230069.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32212..32691)
                     /gene="folA"
                     /locus_tag="RHX88_001358"
     CDS             complement(32212..32691)
                     /gene="folA"
                     /locus_tag="RHX88_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDV2230070.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32883..34745)
                     /gene="kefC"
                     /locus_tag="RHX88_001359"
     CDS             complement(32883..34745)
                     /gene="kefC"
                     /locus_tag="RHX88_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDV2230071.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34738..35268)
                     /gene="kefF"
                     /locus_tag="RHX88_001360"
     CDS             complement(34738..35268)
                     /gene="kefF"
                     /locus_tag="RHX88_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDV2230072.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35376..36707)
                     /locus_tag="RHX88_001361"
     CDS             complement(35376..36707)
                     /locus_tag="RHX88_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2230073.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPSLKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36765..37052)
                     /gene="fixX"
                     /locus_tag="RHX88_001362"
     CDS             complement(36765..37052)
                     /gene="fixX"
                     /locus_tag="RHX88_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDV2230074.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37049..38335)
                     /locus_tag="RHX88_001363"
     CDS             complement(37049..38335)
                     /locus_tag="RHX88_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV2230075.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38386..39327)
                     /locus_tag="RHX88_001364"
     CDS             complement(38386..39327)
                     /locus_tag="RHX88_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV2230076.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKDNIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39342..40112)
                     /locus_tag="RHX88_001365"
     CDS             complement(39342..40112)
                     /locus_tag="RHX88_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDV2230077.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9711 a        11106 c        10605 g         8922 t
ORIGIN      
        1 tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac aaatcatcag
       61 gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat
      121 ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc
      181 ttactttaac caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg
      241 gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
      301 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
      361 ctaaacgcat cttctttgcg cggtagacga gtgagcggca gccagcatta agccagcacg
      421 cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc
      481 taaaagagac aaggacccaa accatgagcc agcaagtcat tattttcgat accacattgc
      541 gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc
      601 tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg
      661 gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt
      721 tagctcgctg cgtggagaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag
      781 ccttccgtat tcataccttt attgccactt cgccaatgca catcgccacc aagctgcgca
      841 gcacgctgga tgaagtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca
      901 ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc
      961 gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg
     1021 gctacaccat gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta
     1081 acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca
     1141 actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg
     1201 gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg
     1261 atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag
     1321 ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcggcgcat
     1381 tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa
     1441 tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg
     1501 ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt
     1561 tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt
     1621 acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc
     1681 tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg
     1741 cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct
     1801 atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg
     1861 ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc
     1921 gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg
     1981 tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag
     2041 ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt
     2101 attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctggatgc
     2161 cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc
     2221 cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga
     2281 tgccgtgctg tttggctcgg taggcggtcc gaaatgggaa catttaccac cagaccagca
     2341 accagaacgc ggcgcgctgt tgcctttgcg taagcacttc aaattattca gcaacctgcg
     2401 cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc
     2461 taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc
     2521 aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg
     2581 ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa
     2641 agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt
     2701 caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc
     2761 caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt
     2821 tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc
     2881 cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga
     2941 tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct
     3001 gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc
     3061 attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga
     3121 tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg
     3181 ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg
     3241 ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt tgatggtctg
     3301 cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac
     3361 gtctctaccc agaccaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag
     3421 gagctgatta aaaactgcaa agaatttggc gtcgagctgt atgacctgaa tcacccgtat
     3481 caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt
     3541 gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc
     3601 ggcacttccg aagttgaaca cgtactggca acacaaaccc tgaaacaggg ccgtgcgaag
     3661 accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttacagcaaa agatatcgtg
     3721 ctggcaatta tcggtaaaac tggcagcgca ggcggcaccg ggcatgtggt ggagttttgc
     3781 ggcgaagcaa ttcgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc
     3841 gaaatgggcg caaaagccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa
     3901 ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc
     3961 ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt
     4021 tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt
     4081 cccgatccgg cttcgtttgc cgatccggtt gaacgcgcgt cggcagaaaa agcgctggcc
     4141 tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc
     4201 ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg
     4261 cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc
     4321 caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg
     4381 cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt
     4441 gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg
     4501 gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac
     4561 attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc
     4621 gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt
     4681 gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg
     4741 ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt
     4801 gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac
     4861 cgactacggt tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag
     4921 ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc
     4981 gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa
     5041 agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa
     5101 cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc
     5161 aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg gctttccgct
     5221 atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa
     5281 taaccgcaat tacccagtag acagcaaagt gcccccaact ttgtgcaatt gctccctgaa
     5341 taacgccagc cagaattacc ccggtagaaa tactgttagt aaataaggtg gtagctgccc
     5401 ccgctcttcc aggcattaaa tcctgaaacc atagcatccc aatacccgca acaatgccga
     5461 taaatacagc gttaaaaagt tgcagcgtca tcaacgccat acggctatga aagaaaatca
     5521 atccggtgta aaacagtact cctgccgcca ctgctatgac catcattcgc cgcttaccat
     5581 aacgtttgac atagtagcca gccagaatca ttgctggtat ttccagtcca gctgccgtcc
     5641 ccatcaggaa acccgccagt ttgtctggca atcctaactc gctactgatc cacaacggca
     5701 tatcaataat gtacatggtg ttgcaggtcc acattaacgt cgaggcgaca aataacatcc
     5761 gtacgttact atcctgccag ccaccttgca ttgataaagc attttccgac ggcagttcta
     5821 cccgcgccac agacggaagc ataaatgcaa tcaataccag actgagtgtg aatatcccgg
     5881 cggcaatcga aaacatcacc gtaaagccgt aattcaacgc cagcataaag gccaacggtg
     5941 gaccgataac ccatgccaga gaaagctgcg cacgcatcac cgagctaaac atcaccactt
     6001 ctcgcgccga gttatccgca tattcccgcg ccagagcaaa taactgtggc attgccgtat
     6061 tggccagaga tgccagaagc acaccacagg tgataagcgt cagataatga cgattaaatg
     6121 caaacaatag cgcattgccg atagccatca aacagcaaaa tataatcagt tttcgccgat
     6181 cgccctgact gtcagaacgt tttgccaacc agaggcttac gccgatccca gcaatagcat
     6241 tcaccgtata aaagagtccg atccagaaag gttgcgcgcc aacctcacga ctcagaaata
     6301 agctcaatgt aggagcctgt agcgccccgg ccacccccat cataaaagcg accagcataa
     6361 atgccgcgta aacaccgttc atacggcgag ccatcgtcat tatccagatc atacgttccc
     6421 tttttagcgc ggcgagaata aaaaaaacca gcaggtataa tctgctggcg ggtgatttta
     6481 caccaatact cagtcacaca tgatgcaggc aagtcaactt tcagaattgc ggtcatccca
     6541 ctgcatcagt ccttcaagca tggttaatcg ttgtgggacg cttaaccaac gcaaccagca
     6601 caacttcgct gtcgcggtaa aatagtgctg ataaaactga cgcatggggc acctccttgc
     6661 ttcatcgtta ggaattattg acttaatata gggaaaataa aattgctgtc ttttgcgcag
     6721 gagttcccct tttatgccat ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg
     6781 ctgcgagggt aaatcgcagg acacaacgct caacgaactg gcagcgttat tgagctgctc
     6841 gcgtcgtcat atgcgtaccc tgctcaacac catgcaggat cgcggctggc tgacgtggga
     6901 agcggaagtc gggcgtggta aacgctcgcg tctgacattc ctctataccg ggctggcgct
     6961 tcagcaacag cgggcggaag acctgctgga gcaggatcgt atcgaccaac tagtgcagtt
     7021 agttggcgac aaagcgactg tgcggcaaat gctggtttct catctgggcc gcagcttccg
     7081 ccaggggcgg cacatcctgc gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg
     7141 cagcgcattg cgccgttccg aaacccatat tgcccggcaa atcttcagtt cgctaacgcg
     7201 cataaatgag gaaaatgggg aactggaagc agacatcgcc caccactggc agcaaatatc
     7261 accgcttcac tggcgtttct ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact
     7321 ggaaatggac gacgtgatcg cctctttaaa acgaatcaat acgctgccgc tctattcgca
     7381 tattgcggac attgtgtcgc cgacgccctg gacgctggat atccatctca cgcaaccgga
     7441 ccgctggtta ccgttactgc tgggacaagt tccggcgatg atcctgccgc gcgaatggga
     7501 aaccctcagt aactttgcca gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa
     7561 caccaccaat caactgaaaa ttcaggcatt cgatgacttc ttcggttacc gggcattaat
     7621 cgatgaagtt aacgtctggg ttctgccgga aattgccgac gagccagctg gaggtctgat
     7681 gctaaaaggt ccacagggcg aggaaaaaga gattgaaagc cgcctggagg aaggttgcta
     7741 ctatttactg ttcgacagcc gcacccatcg cggggcgaat cagcaagtca gggactgggt
     7801 aagctatgtg ctttctccaa ctaatctggt ctatttcgct gaggaacagt accagcaact
     7861 gtggttcccg gcttatggac tgctcccccg ttggcaccat gcccgcacca taaagagcga
     7921 aaaaccggct ggcctggaaa gcctcaccct gaccttttat caggatcaca gtgagcatcg
     7981 ggtgattgcc gggatcatgc agcagattct ggcaagtcac caggtcacgc tggaaatcaa
     8041 agagatcagc tacgatcagt ggcatgaagg agagatcgag agcgatatct ggcttaatag
     8101 cgccaacttt acgctgccgc tggatttttc gctgttcgcg cacttgtgcg aggtgccgct
     8161 gctccaacac tgtattccga tcgactggca agccgacgcc gcccgctggc gcaatggcga
     8221 aatgaacctg gcgaactggt gccagcaact ggtcgccagt aaagcaatgg tgccgcttat
     8281 ccaccactgg ctgatcattc agggacaacg cagtatgcgc ggcctgcgta tgaataccct
     8341 cggctggttc gattttaaat cagcgtggtt tgcgccaccg gatccatgat tgctggtaga
     8401 taacaaaatc actacactaa cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga
     8461 gaaaataccc gtcgaacctg atccggataa cgccggcgaa gggatttgag gctccttctc
     8521 aagtcctttg ccactctttt ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt
     8581 tgctgtgcac agcgcccgtt ttcgctaaac ccgttctgac tgtttatacc tacgattcct
     8641 tcgccgccga ctgggggcct ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact
     8701 gcgaactgaa actggtggcg ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg
     8761 aaggcaaaaa tagtaaagcc gatgtggtgc tggggctgga taacaatctg ttagacgccg
     8821 ccagtaaaac cggactattt gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg
     8881 gcggctggaa taatgacact ttcgtcccgt ttgattacgg ctatttcgcc ttcgtctatg
     8941 acaagaacaa actgaaaaac ccgccgcaaa gcctgaaaga attggttgag agcgatcaaa
     9001 actggcgggt gatttatcag gatccgcgca ccagtacgcc ggggctgggc ctgctgttgt
     9061 ggatgcaaaa agtctatggc gatgacgccc cacaagcctg gcaaaaactg gcgaagaaaa
     9121 ccgtcacggt caccaaaggc tggagcgaag cctacggcct gtttttaaaa ggtgaaagcg
     9181 atctggtact gagttacacc acctctccgg cttatcacat tctcgaagag aaaaaagata
     9241 actacgccgc cgcgaacttt agcgaaggtc actatctgca ggtggaagtc gccgcccgca
     9301 ccgctgccag caagcagccg gagctggcgc aaaaattcct ccagtttatg gtttctccgg
     9361 cttttcagaa tgcgatccca accggtaact ggatgtatcc ggtggcaaat gtcacgctgc
     9421 ctgccgggtt tgaacaattg accaaaccgg ccaccacgct ggagttcaca ccagctgaag
     9481 tggcggcaca acgtcaggca tggattagcg aatggcaacg cgccgtcagc cgttaattcc
     9541 cggctggtta attccaggtg taagcgccgc cacgctggtg gtagcggttg cgctcgcggc
     9601 gtttctcgcc ctgtggtgga acgcgccgca gggtaactgg gtggcagtct ggcaggacag
     9661 ctacctgtgg catgtggtgc gcttctcctt ctggcaggcg tttctctcgg cgctactctc
     9721 tgtcgtaccc gcgatattcc tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct
     9781 ggcgctgttg cgtctgtgcg caatgacctt gatcctcccg gtgctagttg ctgttttcgg
     9841 cattcttagc gtctatggtc gtcagggctg gctggcatcg ctctgccaat cgctcggtct
     9901 ggagtggacc ttttcgccct acggcctgca aggcattttg ctggcgcacg tattttttaa
     9961 tctgccgatg gcgagccgct tattactcca ggcactggaa aacattcccg gcgaacaacg
    10021 tcagcttgcc gcccagcttg ggatgcgcgg ctggcatttt ttccgcttcg tcgaatggcc
    10081 gtggctacgg cgacaaatcc cgccggttgc cgcgcttatc tttatgctct gtttcgccag
    10141 cttcgccacc gtgctatcgc tgggcggcgg tccgcaggca actactatcg agctggctat
    10201 ttatcaggcg ctgagttacg actacgatcc tgcccgcgcg gcgatgctgg cgctgctcca
    10261 gatggtgtgt tgccttgggc tggtgctgtt gagtcagcga ctaagtaagg ccattgcgcc
    10321 cggtaccacg ctgctgcaag gctggcgcga tccggacgat cgtctgcata gccgcatttg
    10381 cgacacggtg ttaatagtgc tggcgctgct gctgttgctg ccaccgttgc tggcggtgat
    10441 cgtcgatggc gtaaatcgcc agttgccgga agtgctggca caaccggtgc tgtggcaggc
    10501 gctgtggacc tcgttgcgta ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat
    10561 gatgctgcta tggagcagtc gcgaactgcg ggcgcggcag aaaatgctgg cgggtcaggc
    10621 gctggagatg agcggcatgt tgatcctcgc catgccgggg attgtgctgg ctaccggctt
    10681 ctttttactg ctcaacaaca ctatcggcct gccacaatct gctgacggca ttgtgatttt
    10741 caccaatgcg ttaatggcga tcccttatgc gctgaaagta ctggaaaacc cgatgcgcga
    10801 tatcaccgcc cgctacagta tgttgtgtca gtcgctgggc attgaaggct ggtcgcgctt
    10861 aaaagtggtc gagctgcgcg ccctgaaacg tccactggcg caggcgctgg cttttgcctg
    10921 cgtgctgtcg attggtgatt ttggcgtagt ggcgttgttc ggtaacgatg atttccgtac
    10981 tctgccgttt tatctctacc agcaaattgg ctcctatcgc agccaggacg gcgcggtcac
    11041 cgcgttaatt ctgctgctgc tctgttttct gctgtttacc gtgattgaaa aactaccggg
    11101 gcgaaatgtt aaaactgact gatatcacct ggctttatca ccatttgccg atgcgtttta
    11161 gcttaacggt ggaacgcggc gagcaggtgg cgatcctcgg gccaagcggc gcgggtaaaa
    11221 gtaccctgct gaatttgatt gccggttttc tgacgccagc cagcggttcg ctgactatcg
    11281 atggcgtaga tcacacaact atgccgccgt cacgccgtcc ggtgtcgatg ctgtttcagg
    11341 agaacaacct gttcagccac ctgacggtcg cgcagaacat cgggctgggg cttaatccag
    11401 gactgaagct gaacgcggca cagcaggaga aaatgcacgc gattgcccgt cagatgggga
    11461 ttgataattt aatggcgcgg ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg
    11521 cgttagcgcg ttgtctggta cgcgaacaac cgattttatt gctcgatgaa ccgttttctg
    11581 cgctcgatcc ggcgctgcgt caggagatgc tgacgctggt gagcacgagc tgccagcagc
    11641 aaaaaatgac gctattgatg gtgtcgcaca gcgtggaaga tgcggcgcgg attgccacgc
    11701 gctcggtggt ggtcgccgac gggcgtatcg cctggcaggg taagaccgat gagttgttga
    11761 gcggtaaggc gagagcttcg gcactattgg ggattacggg ttagtatgca ctgtcggatg
    11821 cggcgtgaac gccttatccg acctacggac agtgcgtttt gtaggcctga taagacgcgg
    11881 caagcgtcgc atcaggcaac ccgccctaaa ccccaaccac tttacgcaaa atatcgatat
    11941 acaccggcat caacgggtgg cgaattaaca ccaccagcgc caccacgccg atggcagaaa
    12001 tcaacggcgt cagccacaac aaacgaccgc gggacaaata atgactcaaa cggtcagtcg
    12061 ctttaccgct gcgccataac cgccagcaca gccagccacc aacccacaaa aataccgctg
    12121 ttgccagcag caaccattta aactcaccgc tctgcattcc ggcaggaata tcgatcgccg
    12181 cgcccgccag aatccccggc aggaagtaaa acggcggcca taacaggcag ccaataatat
    12241 ttggcgtgat aaattttgcc accggcagat ccagcattcc cgccaccatt ggcaccagcg
    12301 gacgcgtcgg gccaacaaag cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg
    12361 catgttcagt tttatcgagt aatgctttgt ttttcttcag aaatgaccag cgatgcaacg
    12421 gctttttaaa acgccagccc agccagaagg agatccagtc gcccagcaga caaccgacga
    12481 tccctgccag ccaggcgtgc cagaaactta actcgccgct gccaatcagc gcacccagcc
    12541 ccgccatcag caccgtaccg ggtagaatca aaccgaccag cgccagcgac tccagaaagg
    12601 ccaccaacac caccgccatc aatgaataca cggtggattg ggtaataaag tgttccagca
    12661 atgcttgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
    12721 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
    12781 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
    12841 aatactcgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
    12901 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
    12961 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggtgacaag
    13021 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
    13081 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
    13141 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
    13201 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
    13261 gcttcatccg ggcgaaagaa acccgtattg gcaaatattg acggccagtt aagccattca
    13321 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
    13381 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
    13441 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
    13501 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
    13561 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
    13621 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
    13681 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta
    13741 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
    13801 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
    13861 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
    13921 tcgcaactct ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat
    13981 tgcaattggc ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg actgcgccag
    14041 cggtgaagag atcgccacca gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg
    14101 tgatgccccg aataaccagt tccgtcatca tccgcgtgac tacattgagt caatggaagc
    14161 ggcactgaaa accgtgcttg cagagcttag cgtcgaacag cgcgcagctg tggtcgggat
    14221 tggcgttgac agtaccggct cgacgcccgc accgattgat gccgacggta acgtgctggc
    14281 gctgcgcccg gagtttgccg aaaacccaaa cgcgatgttc gtattgtgga aagaccacac
    14341 tgcggttgaa gaagcggaag agattacccg tttgtgccac gcgccgggca acgttgacta
    14401 ctcccgctac attggtggta tttattccag cgaatggttc tgggcaaaaa tcctgcatgt
    14461 gactcgccag gacagcgccg tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg
    14521 ggtgccagct ctgctttccg gtaccacccg cccgcaggat attcgtcgcg gacgttgcag
    14581 cgccgggcat aaatctctgt ggcacgaaag ctggggcagc ttgccgccag ccagtttctt
    14641 tgatgagctg gacccgatcc tcaatcgcca tttgccttcc ccgctgttca ctgacacctg
    14701 gactgccgat attccggtgg gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc
    14761 tgaaagcgtg gtgatttccg gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg
    14821 cgcacagcct aacgcactgg taaaagttat cggtacttcc acctgcgaca ttctgattgc
    14881 cgacaaacag agcgttggcg agcgggcagt taaaggtatt tgcggtcagg ttgatggcag
    14941 cgtggtgcct ggatttatcg gtctggaagc aggccaatcg gcgtttggtg atatctacgc
    15001 ctggttcggt cgcgtactca gctggccgct ggaacagctt gccgcccagc atccggaact
    15061 gaaagcgcaa atcaacgcca gccagaaaca actgcttccg gcgctgaccg aagcatgggc
    15121 caaaaatccg tctctggatc acctgccggt ggtgctcgac tggtttaacg gtcgtcgcac
    15181 accgaacgct aaccaacgcc tgaaaggggt gattaccgat cttaacctcg ctaccgacgc
    15241 tccgctgctg ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg caatcatgga
    15301 gtgctttacc gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg
    15361 gaaaaaccag gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt
    15421 tgcctctgac cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt
    15481 gcacgcagac atcccatcag cccagcaaaa aatggccagt gcggtagaga aaaccctgca
    15541 accgcgcagc gaacaggcac aacgctttga acagctttat cgccgctatc agcaatgggc
    15601 gatgagcgcc gaacaacact atcttccaac ttccgccccg gcacaggctg cccaggccgt
    15661 tgcgactcta taaggacacg ataatgacga tttttgataa ttatgaagtg tggtttgtca
    15721 ttggcagcca gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc
    15781 acgttgttaa tgcgctgaat acggaagcga aactgccctg caaactggtg ttgaaaccgc
    15841 tgggcaccac gccggatgaa atcaccgcta tttgccgcga cgcgaattac gacgatcgtt
    15901 gcgctggtct ggtggtgtgg ctgcacactt tctccccggc caaaatgtgg atcaacggcc
    15961 tgaccatgct caacaaaccg ttgctgcaat tccacaccca gtttaacgcg gcgctgccgt
    16021 gggacagtat cgatatggac tttatgaacc tgaaccagac tgcacatggc ggtcgcgagt
    16081 tcggcttcat tggcgcgcgt atgcgtcagc aacatgccgt ggttaccggt cactggcagg
    16141 ataaacaagc ccatgagcgt atcggctcct ggatgcgcca ggcggtctct aaacaggata
    16201 cccgtcatct gaaagtctgc cgttttggcg ataacatgcg tgaagtagcg gtcaccgatg
    16261 gtgataaagt tgccgcacag atcaagttcg gtttctccgt caatacctgg gcggttggcg
    16321 atctggtgca ggtggtgaac tccatcagcg acggcgatgt taacgcgctg gtcgatgagt
    16381 acgaaagctg ctacaccatg acgcctgcca cacaaatcca cggcgaaaaa cgacagaacg
    16441 tgctggaagc ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa ggtggcttcc
    16501 acgcgttcac caccaccttt gaagatttgc acggtctgaa acagcttcca ggtctggccg
    16561 tacagcgtct gatgcagcag ggttacggct ttgcgggcga aggcgactgg aaaaccgccg
    16621 ccctgcttcg catcatgaag gtgatgtcaa ccggtctgca gggcggcacc tcctttatgg
    16681 aggactacac ctatcacttc gagaaaggta atgacctggt gctcggctcc catatgctgg
    16741 aagtctgccc gtcgattgcc gtagaagaga aaccgatcct cgacgttcag catctcggta
    16801 ttggtggtaa ggacgatcct gcccgactga tcttcaatac ccaaaccggt ccagcgattg
    16861 tcgccagcct gattgatctc ggcgatcgtt accgtctgct ggttaactgt atcgacacgg
    16921 tgaaaacacc gcactccctg ccgaaactgc cggtggcgaa tgcgctgtgg aaagcgcaac
    16981 cggatctgcc aactgcttcc gaagcgtgga tccttgctgg tggcgcgcac cataccgtct
    17041 tcagccatgc gctgaacctc aacgatatgc gccagttcgc cgagatgcac gacattgaaa
    17101 tcacggtgat tgataacgac acccgcctgc cagcgtttaa agacgcactg cgctggaacg
    17161 aagtgtatta cggatttcgt cgctaagtag ccacatcagg tatgtaacgc ctgatgcgac
    17221 gctgacgcgt cttatctggc ctacacgctg cgattttgta ggccggataa gcaaagcgca
    17281 tccggcattg ttttccccct cgcccctttg gggagagggc cggggtgagg ggaaacgacc
    17341 tctcaggagt caacatgtta gaagatctca aacgccaggt attagaagcc aacctggcgc
    17401 tgccaaaaca caacctggtc acgctcacat ggggcaacgt cagcgccgtt gatcgcgagc
    17461 gcggcgtctt tgtgatcaaa ccttccggcg tcgattacag cgtcatgacc gctgacgata
    17521 tggtcgtggt tagcatcgaa accggtgaag tggttgaagg tacgaaaaag ccctcctccg
    17581 acacgccaac tcaccggctg ctctatcagg cattcccctc cattggcggc attgtgcata
    17641 cgcactcgcg ccacgccacc atctgggcgc agtcgggtca gtcgattcca gcaatcggca
    17701 ccacccacgc cgactatttc tacggcacca ttccctgcac ccgcaaaatg accgacgcag
    17761 aaatcaacgg cgaatatgag tgggaaaccg gtaacgtcat cgtagaaacc tttgaaaaac
    17821 agggtatcga tgcagcgcaa atgcccggcg ttctggtcca ttcccacggc ccgtttgcat
    17881 ggggcaaaaa tgccgaagat gcggtgcata acgccatcgt gctggaagag gtcgcttata
    17941 tggggatatt ctgccgtcag ttagcgccgc agttaccgga tatgcagcaa acgctgctgg
    18001 ataaacacta tctgcgtaag catggcgcga aggcatatta cgggcagtaa tgactgtata
    18061 aaaccacagc caatcaaacg aaaccaggct atactcaagc ctggtttttt gatggatttt
    18121 cagcgtggcg caggcaggtt ttatcttaac ccgacactgg cgggacaccc cgcaagggac
    18181 agaagtctcc ttctggctgg cgacggacaa cgggccgttg caggttacgc ttgcaccgca
    18241 agagtccgtg gcgtttattc ccgccgatca ggttccccgc gctcagcata ttttgcaggg
    18301 tgaacaaggc tttcgcctga caccgctggc gttaaaggat tttcaccgcc agccggtgta
    18361 tggcctttac tgtcgcgccc atcgccaatt gatgaattac gaaaagcgcc tgcgtgaagg
    18421 tggcgttacc gtctacgagg ccgatgtgcg cccgccagaa cgctatctga tggagcggtt
    18481 tatcacctca ccggtgtggg tcgagggtga tatgcacaat ggcactatcg ttaatgcccg
    18541 tctgaaaccg catcccgact atcgtccgcc gctcaagtgg gtttctatag atattgaaac
    18601 cacccgccac ggtgagctgt actgcatcgg cctggaaggc tgcgggcagc gcatcgttta
    18661 tatgctgggg ccggagaatg gcgacgcctc ctcgcttgat ttcgaactgg aatacgtcgc
    18721 cagccgcccg ctgctgctgg aaaaactcaa cgcctggttt gcaaaccacg atcccgatgt
    18781 gatcatcggc tggaacgtgg tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg
    18841 ttaccgtctt ccgctgcgtc ttgggcgcga taatagcgag ctggagtggc gcgagcacgg
    18901 ctttaaaaac ggcgtctttt ttgcccaggc taaaggtcgg ctaattatcg acggtatcga
    18961 agcgctgaaa tccgcgttct ggaatttctc ttcattctcg ctggaaactg tcgctcagga
    19021 gctattaggc gaaggaaaat ctatcgataa cccgtgggat cgaatggacg aaattgaccg
    19081 ccgtttcgcc gaagataaac ctgcgctggc aacttataac ctgaaagatt gcgagctggt
    19141 gacgcagatc ttccacaaaa ctgaaatcat gccattttta ctcgaacgag caacggtgaa
    19201 cggcctgccg gtggatcggc acggcggttc ggtggcagcg tttggtcatc tctattttcc
    19261 gcgaatgcat cgcgctggtt atgtcgcgcc taatctcggc gaagtgccac cgcacgccag
    19321 ccctggcggc tacgtgatgg attcacggcc agggctttat gattcagtgc tggtgctgga
    19381 ctataaaagc ctgtacccgt cgatcatccg cacctttctg attgatcccg tcgggctggt
    19441 tgaaggcatg gcgcaacctg atccagagca cagtaccgaa ggttttctcg atgcctggtt
    19501 ctcacgggag aaacattgcc tgccggagat tgtgactaac atctggcacg ggcgcgatga
    19561 agccaaacgt cagggtaata agccgctttc gcaggcgctg aagatcatta tgaatgcctt
    19621 ttatggcgtg ctcggcacca ccgcctgccg cttcttcgat ccgcgcctgg catcgtcgat
    19681 caccatgcgt ggtcatcaga tcatgcggca gaccaaagca ttgattgaag cgcagggcta
    19741 cgacgttatc tacggagata ccgactcgac ttttgtctgg ctaaaaggtg cacattcgga
    19801 agaagaggcg gcgaaaatcg gtcgtgtact ggtgcagcac gttaacgcct ggtgggcgga
    19861 aacgctgcaa aaacaacggc tgaccagcgc attagaactg gagtatgaaa ctcatttctg
    19921 ccgttttctg atgccaacca ttcgcggagc cgataccggc agtaaaaagc gctacgccgg
    19981 gctgattcag gagggcgata aacagcggat ggtgtttaaa gggctggaaa ccgtgcgcac
    20041 cgactggacg ccgctggccc agcagtttca gcaggagcta tacctgcgca tcttccgcaa
    20101 cgagccatat caggaatatg tacgcgaaac catcgacaaa ctgatggcgg gtgaactgga
    20161 tgcgcgactg gtttaccgta aacgccttcg ccgtccgctg agcgagtatc agcgtaatgt
    20221 gccgcctcat gtacgcgccg ctcgccttgc cgatgaagaa aaccaaaagc gcggtcgccc
    20281 cttgcaatat cagaatcgcg gcaccattaa gtacgtatgg accaccaacg gcccggagcc
    20341 gctggactac caacgttcac cactggatta cgaacactat ctgacccgcc agctacaacc
    20401 cgtggcggag ggaatactcc cttttattga ggataatttt gctacactta tgaccgggca
    20461 acttgggcta ttttgagcaa aaaaaagagt tcgccagata ccattttgat gcgtgacgaa
    20521 tgctttgcca tccagtacca tagcgccctt tccattcctg gacctgaata acaccactac
    20581 ctcataagca cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat
    20641 atgcctttta cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga
    20701 accgttgtcg cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac
    20761 cgtctgtacg cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc tggtgatact
    20821 attaccagcc atgacggctg gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg
    20881 ctgacctata tcggtactcg cctggatact gaagagtccg gcgtagccct gcgtgaagtt
    20941 ttccttgata gcaaactggt gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt
    21001 gaccgtatgg accgctttgc gctgcgttat cgcgcgcgta agtattccag cgaacagttc
    21061 cgtatgccgt acagcggcct gcgcggtcag cgtaccagcc tgattccgca tcagctcaac
    21121 atcgctcatg atgttggtcg ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt
    21181 ttagggaaaa ccattgaagc cgggatgatc ctgcatcagc aactgctctc tggcgctgct
    21241 gaacgtgtgc taattatcgt cccggaaacc ttacagcatc agtggctggt agaaatgctg
    21301 cgccgtttca acctgcgctt tgcgctattt gatgatgagc gttatgccga agctcagcac
    21361 gatgcttaca acccgtttga caccgaacag ctggtgattt gctcgctgga ttttgcccgt
    21421 cgtagcaaac agcgcctgga acatctctgt gaagccgaat gggacctgct ggtggtcgat
    21481 gaagcgcatc acctggtgtg gagcgaagat gcgccaagcc gtgaatatca ggccattgaa
    21541 caactggcag agcacgtgcc gggcgttctg ctgctgaccg cgaccccgga acagctgggg
    21601 atggaaagcc acttcgcccg tctgcgtctg ctggacccga accgtttcca cgattttgcg
    21661 cagttcgttg aagagcagaa aaattatcgt ccggttgcgg acgccgttgc catgctgctg
    21721 gcaggtaaca aactgagcaa tgacgaactg aacatgctcg gcgagatgat cggcgagcag
    21781 gatatcgagc cgctgttgca ggcagcaaac agcgacagcg aagatgccca gagcgcccgt
    21841 caggagctgg tttcgatgct gatggatcgc cacggcacca gccgcgtgct gttccgtaac
    21901 acgcgtaacg gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa actgccgcta
    21961 ccgacgcagt atcagacggc tattaaagtc tccggcatta tgggcgcacg taaaagtgcg
    22021 gaagatcgtg ctcgcgatat gctctacccg gagcgtattt atcaggaatt tgaaggtgat
    22081 aacgccacct ggtggaactt cgatccgcgc gttgagtggc tgatgggcta cctgaccagc
    22141 catcgctctc agaaagtgct ggtgatctgc gccaaagctg ccactgcgct gcaactggag
    22201 caggtactgc gcgaacgtga aggtattcgc gctgcggtgt tccacgaagg tatgtcgatt
    22261 atcgaacgtg accgcgctgc cgcctggttt gccgaagaag acaccggcgc acaggtactg
    22321 ctgtgctcag aaatcggttc tgaaggacgt aacttccagt tcgccagcca catggtgatg
    22381 tttgacctgc cattcaaccc ggatctactg gagcagcgta ttggtcgtct ggatcgtatc
    22441 ggccaggcgc acgatattca gatccatgtg ccttatctgg agaaaaccgc tcagtcggtg
    22501 ctggtgcgct ggtatcacga aggtctggat gcatttgagc acacctgccc gaccggacgc
    22561 actatttacg atagcgtata caacgatctg attaactatc tggcttcacc ggatcaaacc
    22621 gaaggctttg acgatctgat caaaaactgc cgcgagcaac atgaagcgct gaaagcacag
    22681 ctggaacagg gtcgtgaccg cctgctggaa atccactcca acggtggcga aaaagcccag
    22741 gcactggcag aaagcattga agagcaggat gacgatacca acctgatcgc cttcgccatg
    22801 aacctgttcg atattatcgg tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg
    22861 acgccgtccg atcatatgct ggtgccggac ttccctggcc tgtcggaaga tggcatcacc
    22921 atcacctttg atcgtgaagt ggcactggcg cgtgaagatg cacagtttat tacctgggag
    22981 catccgctga tccgcaacgg tctggatctg atcctttctg gcgataccgg tagcagcacg
    23041 atttcactgt taaaaaacaa agcgttgccg gtaggtacgc tgttggtgga actgatttat
    23101 gtggttgaag cccaggctcc gaagcagttg cagctcaacc gcttcctgcc accgacgccg
    23161 gtacgtatgc tgctggataa aaacggcaac aacctggcgg cgcaggtaga gtttgaaacc
    23221 tttaaccgcc agcttaacgc ggttaaccgt cacaccggca gcaaactggt taacgccgtg
    23281 cagcaggatg ttcacgctat ccttcaactg ggtgaagcgc agatcgagaa atctgcccgt
    23341 gcattgattg atgcagcgcg taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt
    23401 ctggaagctc tgcgtgcagt gaacccgaac attcgtgacg acgaactgac cgccattgag
    23461 agcaaccgtc agcagataat ggaaagcctg gatcaggcag gttggcgtct ggatgccctg
    23521 cgtttgatcg ttgtgacgca tcagtaacgg agctgaaaat ggggatggaa aactacaatc
    23581 caccgcagga accctggttg gttatcctgt atcaggatga ccacattatg gtggtcaaca
    23641 agccgagcgg tttgttgtct gtgccgggtc gtctggaaga gcacaaagac agcgtgatga
    23701 cgcgcattca gcgtgattat ccgcaggcag aatcggtgca tcgtctggat atggctacca
    23761 gcggcgtgat tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc
    23821 gcgagcgcga gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca tcccccgcag
    23881 aaggtctggt ggatctgccg ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct
    23941 gttacgaaac gggtaaacct gcgcagacgg aatatgaagt ggtggagtat gcggcggata
    24001 acacggcaag agtggtgtta aaaccgatta ccgggcgttc gcatcaattg cgtgtgcata
    24061 tgctggcgct ggggcatccg attctcggcg atcgttttta tgcatcacca gaagcgagag
    24121 cgatggcacc gcgtttgttg ctacatgcag agatgttgac gattacccat ccggcgtatg
    24181 gcaatagtat gacgtttaaa gcgccagcgg atttttaatc gtcacgctgg cgcagttcgg
    24241 taggcggata aggcgttcac gccgcatccg gcaattgtgc gccgattgcc tgatgcgacg
    24301 cgggccgcgt cttatcaggc ctacagttta aaacttattt aaaccctttc tgctgcttta
    24361 tcagctcata tgcctgctga atttcctgcg ctttctgctt cgccatctcc atcatctcag
    24421 gcggcaaacc tttcgccacc agcttatcgg gatggtgttc actcatcagc ttacggtagg
    24481 cacgtttgat ggtggtcgca tcatccgtcg gcttcacgcc cagcacatta caggcatctt
    24541 ccagcgtcgg gccacgctgc gcttgctgcc agttaccacc gccagattgc tgctgataac
    24601 cgccgccaaa ctgtgcaccg ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg
    24661 agatccctaa ttcttcagca ataacataca gcaccgcccg ttcattcggg tgcagtgaac
    24721 catcagcaaa cgccgcctga atctggatct ccagaaacat acgaattaag tcaaaacgac
    24781 caaagcagac actgcgaaac tggcgcatct tttcgcgcag cgggtaattg tctgattttc
    24841 ccacccggaa cgcattttgc gccgcagtac gggaagcgcc atgaagattc attcggtcca
    24901 tcaactggct ggcgatatga atatcagcct ccgtgacgcg acctttggat ttggttaaat
    24961 gccccatcac ttcaaaagtg gtggcaaaaa acagcgcctg acgctcacgc tggttggcga
    25021 accacgccat tttacggcta cgggctttat caaacatatg gccaattaac aggcctaaca
    25081 ctacgcccca aaagccgccg cccatcagta aggccacggc cacgccaatg atttttcccc
    25141 aatactgcat atattcccca aatcgacaca cggatatcag ggctatctcc cacaatataa
    25201 aggtgctttt accgttttcc ggctgcggtc aattgtggga catcgcctat aatttgcatt
    25261 atcatacctg tcattcaatg ccgtgcctaa caccactgac gctattcgga caggattaac
    25321 actagcgtag agatgacgag tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg
    25381 atgatggaac aataaaatca acgtatgaaa aaacgtatcc ccactctcct ggccaccatg
    25441 attgccaccg ccctttatag tcaacaggga ctggcagccg acctcgcctc acagtgcatg
    25501 ttgggcgtgc caagctatga ccgtcctctg gtacagggcg ataccaatga cttacccgtg
    25561 actatcaatg ctgaccacgc gaaaggggac tacccggatg acgccgtgtt tactggcagc
    25621 gtggatatca tgcagggtaa cagccgtctg caggccgacg aagtgcagct ccatcaaaaa
    25681 gaggcaccag gacaaccgga gccggtacgt accgttgatg cgctcggtaa tgtccattac
    25741 gacgataacc aggtgatcct caaagggccg aaaggctggg cgaatctgaa caccaaagat
    25801 accaacgtct gggaaggtga ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac
    25861 ctgatgaaac aacgtggcga aaaccgctat accattctgg ataacggtag ctttacctcc
    25921 tgtctgccgg gttctgacac ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa
    25981 gaacaagttg cggagatctg gaacgcccgc tttaaggtgg gtccggtacc gatcttttat
    26041 agcccctatt tgcagttgcc ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac
    26101 gccaagtaca ccaccaccaa ctactttgag ttctacctgc catattactg gaacatcgcg
    26161 ccaaatatgg atgccaccat cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg
    26221 gagaacgaat tccgctacct ctcccaagcg ggcgctggct tgatggaact ggactatctg
    26281 ccttcagata aagtctatga agatgaacac ccgaacgatg acagttcacg tcgttggtta
    26341 ttctactgga accactccgg ggtcatggat caggtgtggc gtttcaacgt cgactacacc
    26401 aaggtcagcg atcctagcta cttcaatgat ttcgataaca agtacggttc cagtactgac
    26461 ggctacgcaa cgcaaaaatt cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt
    26521 tcaaccaagc agttccaggt tttcagcgaa cagaacacca gtagctactc ggcagagccg
    26581 cagttagacg ttaattacta ccagaatgat gttggtccgt ttgatacgcg tatttacggc
    26641 caggcagtgc actttgttaa caccagagac gacatgcctg aagcaacccg tgttcacctg
    26701 gaaccgacca tcaatttgcc gctctctaat aactggggca gcatcaatac cgaagcgaag
    26761 ttgctggcaa cccattatca gcaaaccaat cttgactggt ataactccag aaacacgacc
    26821 aagctggacg aatccgttaa ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc
    26881 tttgaacgcg atatggaaat gctggctccg ggttataccc aaacgctgga accgcgcgcg
    26941 cagtatttgt acgtgccgta tcgcgatcag agcgacatct ataactacga ctcgtctctg
    27001 ctgcaatctg actactctgg cctgttccgg gaccggactt acggcggtct tgaccgtatt
    27061 gcctccgcta accaggtgac gaccggtgtc acatctcgca tatatgatga tgctgccgtt
    27121 gaacgtttta atatttccgt tggtcaaatc tactatttca cggagtctcg cactggcgat
    27181 gacaacataa catgggagaa tgacgacaaa acgggttcac tggtgtgggc aggcgatact
    27241 tactggcgta tctccgagcg ttggggattg cgtggcggga ttcagtacga tacacgtctg
    27301 gataacgtag cgaccagtaa ctccagcatt gaataccgtc gggatgaaga ccgtctggta
    27361 cagctgaatt accgttacgc cagcccggaa tatattcagg ctacgctgcc taagtactat
    27421 tccactgctg agcaatataa gaatggtatt tcgcaggtag gtgctgtcgc cagctggcca
    27481 attgccgatc gttggtccat tgttggggcc tactactacg acaccaatgc gaacaagcaa
    27541 gccgactcta tgttaggtgt gcaatacagc tcctgctgct atgcaattcg cgtcggttac
    27601 gagcggaagc tgaacggttg ggataacgat aaacaacatg cggtatatga caacgcaatc
    27661 ggctttaaca tcgaacttcg cggcctgagc tccaactacg gtctgggtac gcaagagatg
    27721 ctgcgttcga acattctgcc gtatcaaaac actttgtgat ttgttgattt accacgtaat
    27781 ccgcagtgcg gttaattgaa atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg
    27841 tatcgccatg atcgcgaata ccagtttcgc tgccccccag gtagtcgata aagtcgcagc
    27901 cgtcgtcaat aacggcgtcg tgctggaaag cgacgttgat ggattaatgc agtcggtaaa
    27961 actgaacgct gctcaggcaa ggcagcaact tcctgatgac gcgacgctac gccaccaaat
    28021 catggaacgt ttgatcatgg atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa
    28081 aatctccgat gagcagctgg atcaggcgat tgctaacatc gcgaaacaga acaacatgac
    28141 gctggatcag atgcgcagcc gtctggctta cgatggactg aactacaaca cctatcgtaa
    28201 ccagatccgc aaagagatga ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat
    28261 caccatcctg ccgcaggaag tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc
    28321 cagcactgag ctgaacctga gccacatcct gatcccgctg ccggaaaacc cgacctctga
    28381 tcaggtgaac gaagcggaaa gccaggcgcg cgccattgtc gatcaggcgc gtaacggcgc
    28441 tgatttcggt aagctggcga ttgctcattc tgccgaccag caggcgctga acggcggcca
    28501 gatgggctgg ggccgtattc aggagttacc cgggatcttc gcccaggcat taagcaccgc
    28561 gaagaaaggc gacattgttg gcccgattcg ttccggcgtt ggcttccata ttctgaaagt
    28621 taacgacctg cgcggcgaaa gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat
    28681 tctgctgaaa ccgtcgccga tcatgactga cgaacaggcc cgtgtgaaac tggaacagat
    28741 tgctgctgat atcaagagtg gtaaaacgac ttttgctgcc gcagcgaaag agttctctca
    28801 ggatccaggc tctgctaacc agggcggtga tctcggctgg gctacaccag atattttcga
    28861 tccggccttc cgtgacgccc tgactcgcct gaacaaaggt caaatgagtg caccggttca
    28921 ctcttcattc ggctggcatt taatcgaact gctggatacc cgtaatgtcg ataaaaccga
    28981 cgctgcgcag aaagatcgtg cataccgcat gctgatgaac cgtaagttct cggaagaagc
    29041 agcaagctgg atgcaggaac aacgtgccag cgcctacgtt aaaatcctga gcaactaatg
    29101 gttaaaaccc aacgtgttgt gatcactccc ggcgagcccg ccgggattgg cccggactta
    29161 gttgtccagc ttgcacagcg tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact
    29221 ctccttaccg accgggcagc gatgctcggt ttgccgctca ccctccgccc ttattccccc
    29281 aactcccctg cacaaccgca aactacgggc acattaacgc tacttcctgt cgcgctacgt
    29341 gaatctgtca ctgcggggca gttagcgatt gaaaatggac attacgtggt ggagacgctg
    29401 gcgcgagcgt gtgatggctg tctgaacggt gaatttgctg cgctgatcac aggccccgtg
    29461 cataaaggcg tcattaacga cgcaggcatt ccgtttaccg gtcataccga gtttttcgaa
    29521 gagcgttcgc aggcgaaaaa agtggtgatg atgctggcga ccgaagaact tcgcgtggcg
    29581 ctggcaacga cgcatttacc gctgcgcgat atcgcagatg ctatcacccc tgcacttttg
    29641 cacgaagtga ttgctatttt gcatcacgat ttgcgtacaa aatttggtat tgccgaaccg
    29701 cgcattctgg tctgcgggct gaatccgcac gcgggcgaag gcggtcatat gggtacggaa
    29761 gagatagaca ccattattcc ggtgctcgac gagctgcggg cgcaggggat gaaactcaac
    29821 gggccgctgc ctgccgatac cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg
    29881 ctggcgatgt accacgatca gggtcttccc gtgctaaaat accagggctt cgggcgcggt
    29941 gtgaacatta cgctgggcct gccctttatt cgcacatcag tggaccacgg caccgcgctt
    30001 gaactggcgg gacgtggcga agccgatgtc ggcagtttta ttacggcgct taatctcgcc
    30061 atcaaaatga ttgttaacac ccaatgaata atcgagtcca ccagggccac ttagctcgta
    30121 aacgcttcgg gcaaaacttt ctcaacgatc agttcgtgat cgacagcatt gtttctgcca
    30181 ttaacccgca aaagggccag gcaatggtcg aaatcggccc cggtctggcg gcattgaccg
    30241 agccggtcgg cgaacgtctg gaccagctga cggtcatcga acttgaccgc gatctggcgg
    30301 cacgtctgca aacgcatcca ttcttaggcc cgaaactgac gatttatcag caggatgcga
    30361 tgacctttaa ctttggcgaa ctggccgaga aaatgggtca gccgctgcgt gttttcggca
    30421 acctgcctta taacatctcc acgccgttga tgttccatct gtttagctat actgatgcca
    30481 ttgccgacat gcactttatg ttgcaaaaag aggtggtgaa tcgtctagtt gcaggaccga
    30541 acagcaaagc gtatggtcga ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg
    30601 tactggaagt tccgccgtca gcctttacac caccacccaa agtggattcc gccgtcgtgc
    30661 gcctggttcc tcatgcaacg atgcctcacc cggttaaaga tgttcgtgtg ttgagccgca
    30721 tcaccaccga agcctttaac cagcgtcgta aaaccattcg taacagcctc ggcaacctgt
    30781 ttagcgccga ggtgttaacg ggaatgggga tcgacccgtc gatgcgagcg gaaaatatct
    30841 ctgtcgcgca atattgccag atggcgaact atctggcgga gaacgcgcct ttgcaggaga
    30901 gttaacgatg atcaattcgc cccgagtgtg tattcaggtt caaagcgtct acattgaggc
    30961 acaatcttca cctgataatg aacgttacgt ttttgcttat accgtaacca tacgcaatct
    31021 ggggcgagcg ccagtgcagt tgttggggcg ttactggctg atcaccaatg gcaatggccg
    31081 tgaaaccgaa gtccagggcg aaggagtggt tggcgtccag ccacttatcg cgcctggcga
    31141 agagtaccag tacaccagcg gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca
    31201 ctacgaaatg atcgatgaaa atggcgtccc tttcagcatc gacattcccg tattccgact
    31261 cgccgttccc acactcattc attaaaagaa tatggcgaca taccttattg gcgacgttca
    31321 tggttgttac gatgaactga tcgcattgct gcataaagta gaatttaccc ctgggaaaga
    31381 taccctctgg ctgacgggcg atctggtcgc gcgcggcccg ggttcgctgg atgttctgcg
    31441 ctatgtgaaa tccttaggcg acagcgtacg tctggtgctg ggcaatcacg atctgcatct
    31501 gctggcggta tttgccggga tcagccgcaa taaaccgaaa gatcgcctga caccgctgct
    31561 ggaagcgccg gatgccgacg agctgcttaa ctggctgcgt cgccagcctt tgctgcaaat
    31621 cgacgaagag aaaaagttgg tgatggccca cgcagggatc acgccgcagt gggatctaca
    31681 gaccgccaaa gagtgcgcgc gcgatgtaga agcggtgcta tcgagtgact cctatccctt
    31741 ctttctcgac gctatgtacg gcgatatgcc aaataactgg tcaccggaat tgcgggggct
    31801 gggaagactg cgttttatca ccaacgcctt tacccgtatg cgtttttgct tcccgaacgg
    31861 tcaactggat atgtacagca aagaatcgcc ggaagaggcc cctgccccac tgaaaccgtg
    31921 gtttgcgatt cctggccctg tcgctgaaga atacagcatc gcctttggtc actgggcatc
    31981 gctggagggc aaaggtacgc cggaaggtat atacgcgctg gataccggct gctgctgggg
    32041 tggtacatta acctgcctgc gctgggaaga taaacagtat tttgtccagc cgtcgaaccg
    32101 gcataaggat ttgggcgaag cggcggcgtc ttaaacacag cctgatatag gaaggccgga
    32161 taagacgcga ccggcgtcgc atccggcact agccgtaaat tctatacaaa attaccgccg
    32221 ctccagaatc tcaaagcaat agctgtgaga gttctgcgca tcagcatcgt ggaattcgct
    32281 gaataccgat tcccagtcat ccggctcgta atccgggaaa tgggtgtcgc cttccacttc
    32341 tgcgtcgata tgcgtcagat acagtttttg cgcttttggc aagaactgtt cataaacgcg
    32401 accgccgcca atcaccatga tttctggtac gtcaccacac gccgcgatgg cttcatccac
    32461 cgacttcacc cacgttacgc gatcgtccgt acccggttga ctgctgagga taatattttt
    32521 gcgtcctggc aacggacgac cgattgattc ccaggtatgg cggcccataa tcacgggttt
    32581 atttaaggtg ttgcgtttaa accaggcgag atcggcaggc aggttccacg gcatggcgtt
    32641 ttccatgccg ataacgcgat ctaccgctaa cgccgcaatc agactgatca ttgagatttc
    32701 ccgataaaaa aaattgtcgc cactatacgt aaagcgtaaa ccgtcgtcga ctggtgcgag
    32761 gatgatgttg aggaaaattt tatattctgc tggcgagtcc acgctctctc cctggactcg
    32821 ccgcattaca atgaaacaaa aacaaacagt tagctgtaaa gtgtgattta cgtcactctt
    32881 tattaggatg agggtttcgt ttccggttca tccgccatgt tgccggtatg tttaccttct
    32941 tcggttccct gccagccatg tcgttgaatt aatgacagat gttcgcggtc ctcggtaatg
    33001 atctcactta acatcgcgct ggtgcgttta tagaccgccg cgcgggcttt ggtgtcgttc
    33061 tcaaccattg ccatctcttc caccatctga atattaaagc ggcggaacac atcggcacgt
    33121 tctcgcgctt catacggccc cagacctaaa ctttccagtg ccagacgccc ggttttcagc
    33181 gcaccttcga aggtttcacg ctccggcttt tcaacgcctg cctgacgcaa acgaatgtag
    33241 tggtcgacat cgcgggcgcg ggcaataatc tgcaaatgcg ggaaatgttc tttcaccatc
    33301 tctgtcagtt gcaggttggt ttgcggatcg tcgatggcgt taatcagcac ttccgctttc
    33361 gccgctccgg cagattccag taaatccatc cgcgtggcat cgccataaaa cactttcata
    33421 ccaaatttac gcaaggtttc gatatggtcc ggatcgtgat cgagtaccac cattttcacc
    33481 ccgctggaga gcagtaaacg tccggtaatc tgcccaaaac gaccgaatcc ggcgataatc
    33541 acgcgcggct gttcttcgtc gatctcatcg gcttcacgcg cttcctcagt agaagattgc
    33601 tcaaggcgat tgaggatcac cagcagaatc ggcgttgctg ccatcgacag cgccaccgcc
    33661 agggtcagcg atttcgccca ctccggctcc agcacattcg ccatctgcgc cgcgccaaat
    33721 accacaaagg caaactcact gccctgccct aacaacaccg caaaccaacg acgctgttta
    33781 tttggcactt gcaacggtcg ggcaatcagc cacagcatgg cgattttgat gatgaggaaa
    33841 ccgagcagca aaatgacaat gcgcaatggg ttttcaagca gcgtgccaaa gtctatcgac
    33901 atgccaacac cgatgaaaaa cagccccaac agcaaacctt taaatggttc gatatcgctc
    33961 tccagcgcat gacggtattc cgagcttgcc agcagtacgc ccgccagaaa cgcgcccatc
    34021 gccatcgaca agccgacctc ttccagcagc aaaccaaagc caaacacgag gaataacgcc
    34081 acggcactaa acacttcccg caagccagag cgggctacaa aacgcagcgc cggacgcgtg
    34141 acatagcgcc ccagcaatac caccagcacc agcgcacccg ccacttttaa cgccgagaga
    34201 gcaaatgcgc ccatcgtcgt cgaggcactg ctcgttgcca gtagcggaat catcgccacc
    34261 agcgggatcg ccgcgatatc ctggaacagc agcaccgcaa aggcactgcg acccatttgc
    34321 gtcaccatca gattgcgttc attcatcgcc tgcatggcaa tcgccgtaga ggagagcgcc
    34381 agcgtcatgc cgatcaattc cgcgacctgc cagcgcagcc caagtaacat gcagaacagc
    34441 cccagcaggc caccgcaaat caccatctgc aatgcgccgc cgccgaacac tgccgcacgc
    34501 agcttccata gcctttgtgg atcgagttcg aggccgataa taaacagcat cagcaccacg
    34561 ccaatctcgg caaagtgcag gatagattcg gcatcggtca ccagtcgcag cccccacggg
    34621 ccaataatgc agccggcaat caggtagcca agtaccgatc ccagaccaag acgtaccgca
    34681 atgggtacaa tcagcgccgc cgatccgagg tagatcagcg cctgaaccag cgtatggcta
    34741 tccatgatgg gcctcctgcc attccagcag acgttgctta tagtgacgcg cctgcccttc
    34801 gagggtttcg tcgtcacaaa taaaggtgca gtgcatggca aacggtggca gccagttcag
    34861 cccgcagtag attgccgtcg cctgtagcgg ctgcgacagc acatcaaagc ccggatgcgc
    34921 accaatttca aaatggcttt ccccgccgcc ggtcgtcacc gcccacagca aatgtttgcc
    34981 atgcagcgcc gtgccgccgt gaccgtaagc ccagccgtgc gagaaaactt tatcgatcca
    35041 aagtttgagg agcggtggaa tgctgtacca ctgcatcggg tgctgccaga cgatcagatc
    35101 ggcgcgagac agcgcctcct gctcggcggc aatatcgata ttgaagtcag gatagagttg
    35161 ataaagagag cgaatttcga cgccttccag cgtccttgcc tgttcaagca tccgtttatt
    35221 cgcatgggaa tgatgcggat acggatgcgc ataaattata agaatcatag gtagcccgcg
    35281 tgattacgcc tttgttttcc cacaaagtgt agtcatatat tcacgcggct tatagtcaat
    35341 aatattgatt cagaaaagag agaaatctga acaatttacc ccattctctg cccgcggatc
    35401 gtcatattgc tggtttgcgc cagtgacatc cctcgagtct ccggggcaaa cgctacggaa
    35461 atcaacaagc caaacagcga gatacccgcc cccattagca tcgtgttact gataccgtaa
    35521 ttattgataa agatcggcag tgcccaggtc gaaacaatgg tgccaatacg gcttaaggac
    35581 ataatcacgc ccacggcaga ggcgcggata tctgtcggga agagttcatt aggatagagc
    35641 cactgcaaat tacccggccc accagagaaa aaggcataca ccgcaaaggc catcactacc
    35701 agccagatcc ccatatccgg gatcagcccc aaaaccgcca gcgccagcgt catcatggca
    35761 aagctgccaa tcaacaatgg acgccgtccg gcagtgttta gccacagcat cggcggaata
    35821 cagccgagca taaagaacag gctaatcacc acgttcccca gtgccgcgtt tttgccaacc
    35881 cccaatccca acaaaccaac gatttgcggg ccaaaggtgt aaatggcgaa cattgggatc
    35941 acctggcagg tccagatggc ggcaacaaac agaacaaaag gaaaatggcg gcgattaaac
    36001 agatcgcgaa aacgggtttg ctgcggctgc tcttcatcga aagccaccgg ttcgccaaac
    36061 agttttatca tcatctcttc gcactctttt actcgccctt tgcgtaataa ccagcgggga
    36121 gattcaggca gttcgaatcg accaatcaaa atcaacaaac aggggatcgc cgcgctaccc
    36181 agcatccagc gccagccgcc ttccacatca taaagccagt agccgaccag atcggcgcag
    36241 gtcgcgccga cataccacat ggcggcgata aagctgatgg aaaacgcccg ctgacgggta
    36301 ctggagaact cggtgatcat tgaggtggcg atgggataat ctgcaccgat gacaatgccg
    36361 ataagtaccc gcatcaccaa cagttcgaca ggggatgaga caaacatcgt cgccaccgat
    36421 atcacgccga tggcgatgat atcaatgagg aacattttgc gccgtccgac tttatcggag
    36481 atatagccaa acagcgatgt gccaacgaac agcccggcga gcgttcccgc acccagcaag
    36541 ccaatccagt cagcgtccag tttcagcgac ggagtcagtt gctccagcgc cacgccaatc
    36601 attaccagta cataaccatc cagaaacgga ccaccacttc cccacagcaa aatgcggcgg
    36661 tgaatagagg agaatttgag atcgtcaaag tttctggacg gttgcatggg ttattcctgt
    36721 tttttgcaat gagagtcagt gcggcgttac gtatcaaacc aacatcagcc gtaacggaac
    36781 tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc cagccccaga
    36841 atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact gccgtcatcc
    36901 tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagggc ctgtttatcg
    36961 gcatcggcct tcacaacaat gtgtggatgc tcttcatcga cattgaattt attgacgccc
    37021 agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc atgccatcct
    37081 tgatcagatt gatgaagccc actttcttgc cgtggcggag gattttcttg cgcatcagtt
    37141 ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc agctccgggt
    37201 agccgctgaa catgcgtggg ttatcaagga acgccggtag tttctggtac atgcgcatat
    37261 cgcgcagcgg gccactctca agatgctgac gatattccgc cagtttttgc ttactgaaat
    37321 cgtcgctttt catcgctgaa agcacggttt ttgctgcagc ttccccggcg gcaatcgcca
    37381 gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca tcgccggcaa
    37441 tcaatacgcc gtcaccgacc agctccggca gcatgttgat gcctgcttcc ggcactacgt
    37501 gagcggaata ttccaccagc ttgccacccg cgatcagcgg tgcaacggcc ggatgttgtt
    37561 tgaaatcttc cagcatttgc ggcaccgatt tttttgcgtc atgcagatga tgcagaccac
    37621 aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg cccatcaggc
    37681 catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc aactgaaaac
    37741 ggtcttcgat taccgacttc ggtaactcga tcagttcctt cacgccaacc gccacatccg
    37801 tcggtttaac gcgttttgcc atccccaact tttcggcaag gatggaattc accccgtcag
    37861 caaggatcac cgttttcgct tcaatcacat cgccatcggc ttctacaccg acgactttgc
    37921 catcgcgctg tacgaggtta tcgacacgaa tcccggtaat taactgcgcg cccgcttctt
    37981 cggcctgctc catcaaccag gcatcaaatt tactgcgcaa aacggagtaa gaacgctggg
    38041 atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt tccgtcataa
    38101 acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggagtcggcg aaaccaggaa
    38161 taatgtgttc cagactgtgg gcatagagac gcccgccggt gacgttcttg gcacctgcgg
    38221 aattgccacg ctcgataact aacacttgcg caccttcgcg ggcgagcacc agtgcggcaa
    38281 ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa gatatcttcg gacataagag
    38341 ctccctgtca gcggcagggc caaaatgcag ccctgccaga gtggatcaac gcgctaaagc
    38401 tgcggtcagc gccggaagga tcttcacggc gtcgccaaca atgccgtaat ccgcgtactg
    38461 gaagatcggc gcatttttat ctttgttgat ggcgaaaatg gtttgcgatg cattagcacc
    38521 aaccatgtgc tggatctgcc cggagatccc caccgccagg tacagttcag gtttcagcat
    38581 caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt ccgccaccgg
    38641 acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca gcgcaatgtt
    38701 atctttgctg ccaatgccgc gaccgacgct gaccaccaga cgggctttgt cgagatcgac
    38761 gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg gagcctgcca
    38821 ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat cgaacgtacc
    38881 gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa tcgccagacc
    38941 accgtaaacc atgtgtttca ctgtcgcttt accgtcctgt acgctgacgg tgctggcatc
    39001 gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt tgccgcgacg
    39061 ggtgtttggc agcagcacca ggccgtctgc gccgtgctgg cgaatagtgt cagccatgac
    39121 accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc agacatgatt
    39181 agcgccgagc tggattgcct gtgcgccgtc ggcatcattg aggacaaagg cattgatttg
    39241 attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaagggg tatcgctgaa
    39301 tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac tttgcgaaga
    39361 ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat gcgctgacgt
    39421 tcgcgctgtt tcggcgcggc aacctgttgt tctgaccagg ctgcctctgc gttaaaacca
    39481 atatccgccg ccgaccatac ctggacgggc tttttcgccg cgccgagaat ggctttcatc
    39541 gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg cagcggaatg
    39601 cttaaggttt cggtttcatc ttccagttcg cgctcaacgg tgagggtatc tgccgtcagg
    39661 gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc caccagcaga
    39721 ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcaa atcaaagcct
    39781 gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc ctgctcgaac
    39841 tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg atagcacatc tttacgccct
    39901 ttggcgttgg tcagggcttt accgcccaca cttaaggccg tcacctgcgc ctctgctgcc
    39961 tgttgcttta gctggcaagc cgcttcaata gcgttcagat cgtattggct tattttggca
    40021 tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc ctgttcatca
    40081 ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat gaaggaaacg
    40141 tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga aataaaactt
    40201 taatcaccaa tattgaaaat gtcacacgca gataaatcca actttcaata ttgttaagtt
    40261 cctcaccaat attgaaaaca tggcgtagaa aaaagaaatt ttcaatattg ctttatggat
    40321 caccaatatt gaaagtcaga tgct
//
LOCUS       DAPGWD010000027        40330 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPGWD010000027 DAPGWD010000000
VERSION     DAPGWD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.027"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            215..739
                     /gene="lpfA1"
                     /locus_tag="RHX88_001366"
     CDS             215..739
                     /gene="lpfA1"
                     /locus_tag="RHX88_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HDV2230078.1"
                     /translation="MKKVVFALTALALTSGSAFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADQSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            804..1490
                     /gene="lpfB"
                     /locus_tag="RHX88_001367"
     CDS             804..1490
                     /gene="lpfB"
                     /locus_tag="RHX88_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone LpfB"
                     /protein_id="HDV2230079.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSATRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFRLDPGKNNILRIVNTTPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLQGDVKTAPNELRFTRSGNQI
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGSLNITLPAGAGSVSKIKYNTINDFGS
                     SAEMMTKNVD"
     gene            1519..4092
                     /gene="lpfC"
                     /locus_tag="RHX88_001368"
     CDS             1519..4092
                     /gene="lpfC"
                     /locus_tag="RHX88_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HDV2230080.1"
                     /translation="MSRKTVSRTFSSFSISVVAVAVASTFSAHAGKFNPKFLEDVQGV
                     GQHVDLTMFEKGQEQQLPGIYRVSVYVNEQRMETRTLEFKEATEAQRKAMGESLVPCL
                     SRTQLAEMGVRVESFPALNLVPAEACVPFDEIIPQASSHFDFSEQKLVLSFPQAAMHQ
                     VARGTVPESLWDEGIPALLLDYSFSGSNSEYDSTGSSSSYVDDNGTVHHDDGKDTLKS
                     DSYYLNLRSGLNLGAWRLRNYSTWSHSGGKAQWDNIGTSLSRAIIPFKAQLTMGDTAT
                     AGDIFDSVQMRGAMLASDEEMLPDSQRGFAPIVRGIAKSNAEVSIEQNGYVIYRTYVQ
                     PGAFEINDLYPTANSGDLTVIIKEADGSEQRFIQPFSSVPIFQREGHLKYSFAAGEYQ
                     AGNYDSASPRFGQLDLIYGLPWGMTAYGGVLISNNYNAFALGIGKNFGYIGAISIDVT
                     QAKSELNNDRDSQGQSYRFLYSKSFESGTDFRLAGYRYSTSGFYTFQEATDVRSDADS
                     DYNRYHKRSEIQGNLTQQLGAYGSVYLNLTQQDYWNDAGKQNTVSAGYNGRIGKVSYS
                     IAYSWNKSPEWDESDRLWSFNISVPLGRAWSNYRVTTDQDGRTNQQVGVSGTLLEDRN
                     LSYSVQEGYASNGVGNSGNANVGYQGGSGNVNVGYSYGKDYRQLNYSVRGGVIVHSEG
                     VTLSQPLGETMTLISVPGARNARVVNNGGVQVDWMGNAIVPYAMPYRENEISLRSDSL
                     GDDVDVENAFQKVVPTRGAIVRARFDTRVGYRVLMTLLRSAGSPVPFGATATLITDKQ
                     NEVSSIVGEEGQLYISGMPEEGRVLIKWGNDASQQCVAPYKLSLELKQGGIVPVSANC
                     Q"
     gene            4107..5168
                     /locus_tag="RHX88_001369"
     CDS             4107..5168
                     /locus_tag="RHX88_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2230081.1"
                     /translation="MKAAIALSLLGCVFGFSGKALAGGSWGPCTPVDGTTYHYNVDVD
                     VGVPDASKNVAGTVLTDVLNWSNGQNVSIICECPDKGDYKIEKDTLIQGVSGIANMTR
                     DIGGFKYYYLTDELSVSTKIQVSSGQYGFVPFKGQQALQVTGCNEVITTPYMGGGGLL
                     SFLITKPFIGDSVIPPTLIAELYASKTNKDYGTIPISSVSIQGRVTITQDCEIKPGTV
                     LDVPFGEFPSSAFKNRQGQMPEGATEQEINLGFDCNNISDGIKVSLRLEGATNADDPR
                     AVDMGNPDIGVLVKDSSGKILVPNDASSTTLLNLSSLDSKTHRNAAIRLLALPISTTG
                     KAPKGGTFEGVTTIYLEME"
     gene            5173..5703
                     /gene="lpfE"
                     /locus_tag="RHX88_001370"
     CDS             5173..5703
                     /gene="lpfE"
                     /locus_tag="RHX88_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HDV2230082.1"
                     /translation="MKFNLLLHSGIASLSLFACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5958..7649
                     /gene="eptB"
                     /locus_tag="RHX88_001371"
     CDS             5958..7649
                     /gene="eptB"
                     /locus_tag="RHX88_001371"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDV2230083.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAVAN"
     gene            7741..7817
                     /locus_tag="RHX88_001372"
     tRNA            7741..7817
                     /locus_tag="RHX88_001372"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7775..7777,aa:Pro,seq:cgg)
     gene            8561..10168
                     /gene="dppA"
                     /locus_tag="RHX88_001373"
     CDS             8561..10168
                     /gene="dppA"
                     /locus_tag="RHX88_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDV2230084.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10476..11495
                     /gene="dppB"
                     /locus_tag="RHX88_001374"
     CDS             10476..11495
                     /gene="dppB"
                     /locus_tag="RHX88_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDV2230085.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11505..12407
                     /gene="dppC"
                     /locus_tag="RHX88_001375"
     CDS             11505..12407
                     /gene="dppC"
                     /locus_tag="RHX88_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDV2230086.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12418..13401
                     /gene="dppD"
                     /locus_tag="RHX88_001376"
     CDS             12418..13401
                     /gene="dppD"
                     /locus_tag="RHX88_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDV2230087.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13398..14402
                     /gene="dppF"
                     /locus_tag="RHX88_001377"
     CDS             13398..14402
                     /gene="dppF"
                     /locus_tag="RHX88_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDV2230088.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14432..15703)
                     /locus_tag="RHX88_001378"
     CDS             complement(14432..15703)
                     /locus_tag="RHX88_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV2230089.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16179..16286
                     /locus_tag="RHX88_001379"
     CDS             16179..16286
                     /locus_tag="RHX88_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV2230090.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16662..16769
                     /locus_tag="RHX88_001380"
     CDS             16662..16769
                     /locus_tag="RHX88_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV2230091.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17145..17252
                     /locus_tag="RHX88_001381"
     CDS             17145..17252
                     /locus_tag="RHX88_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV2230092.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17339..19018)
                     /gene="bcsG"
                     /locus_tag="RHX88_001382"
     CDS             complement(17339..19018)
                     /gene="bcsG"
                     /locus_tag="RHX88_001382"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDV2230093.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMVQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19015..19206)
                     /gene="bcsF"
                     /locus_tag="RHX88_001383"
     CDS             complement(19015..19206)
                     /gene="bcsF"
                     /locus_tag="RHX88_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDV2230094.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            19358..19558
                     /locus_tag="RHX88_001384"
                     /pseudo
     CDS             19358..19558
                     /locus_tag="RHX88_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19565..20671
                     /gene="bcsZ"
                     /locus_tag="RHX88_001385"
     CDS             19565..20671
                     /gene="bcsZ"
                     /locus_tag="RHX88_001385"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDV2230095.1"
                     /translation="MNVLRSGLVTMLLLAAFSIQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALSANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            20653..24126
                     /gene="bcsC"
                     /locus_tag="RHX88_001386"
     CDS             20653..24126
                     /gene="bcsC"
                     /locus_tag="RHX88_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDV2230096.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKTNNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            24208..26196
                     /gene="hmsP"
                     /locus_tag="RHX88_001387"
     CDS             24208..26196
                     /gene="hmsP"
                     /locus_tag="RHX88_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDV2230097.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLQEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            26379..27665
                     /gene="dctA"
                     /locus_tag="RHX88_001388"
     CDS             26379..27665
                     /gene="dctA"
                     /locus_tag="RHX88_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDV2230098.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            27886..29382
                     /locus_tag="RHX88_001389"
     CDS             27886..29382
                     /locus_tag="RHX88_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV2230099.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(29478..30407)
                     /gene="kdgK"
                     /locus_tag="RHX88_001390"
     CDS             complement(29478..30407)
                     /gene="kdgK"
                     /locus_tag="RHX88_001390"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDV2230100.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            30639..31406
                     /gene="pdeH"
                     /locus_tag="RHX88_001391"
     CDS             30639..31406
                     /gene="pdeH"
                     /locus_tag="RHX88_001391"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDV2230101.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            31476..33536
                     /locus_tag="RHX88_001392"
     CDS             31476..33536
                     /locus_tag="RHX88_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV2230102.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKNANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLTLRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPIIDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(33718..35040)
                     /locus_tag="RHX88_001393"
     CDS             complement(33718..35040)
                     /locus_tag="RHX88_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV2230103.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(35433..36446)
                     /gene="yhjD"
                     /locus_tag="RHX88_001394"
     CDS             complement(35433..36446)
                     /gene="yhjD"
                     /locus_tag="RHX88_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDV2230104.1"
                     /translation="MTQENEIKRPTQDLEHEPIKPLDNSEKGGKVSQALETVTTTAEK
                     VQRQPIIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISILANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(36495..37394)
                     /locus_tag="RHX88_001395"
     CDS             complement(36495..37394)
                     /locus_tag="RHX88_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2230105.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            37914..38516
                     /locus_tag="RHX88_001396"
     CDS             37914..38516
                     /locus_tag="RHX88_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV2230106.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(38567..40216)
                     /locus_tag="RHX88_001397"
     CDS             complement(38567..40216)
                     /locus_tag="RHX88_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV2230107.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         9806 a        10023 c        10972 g         9529 t
ORIGIN      
        1 gtatttgttt tttcctaaaa atcattcctg attttttatc ccgaaaggca acttagtgat
       61 ttaaatcaaa attcacactt tcttaattta ttgatatttt atcctcttaa ttttcttttc
      121 cttgtttatt tgtataatcc cgccttcaaa gttgatgcaa tgttaaagca tcactatttg
      181 ctaattaatg tgatttaata aaatggagtt tttcatgaaa aaggtcgttt ttgccttaac
      241 tgcactggcg ttaacctccg gctctgcatt tgcagcagaa tctggtgatg gcacggttaa
      301 atttacaggc gagatcgtgg attcaccttg cgtactgtcc gttgactctc agaaccagga
      361 agttgtgttg ggtcaggtac aaaaatctgt atttgctgcg gttggtgaca aatccccggc
      421 aaaacctttc gaaatcaaac tggaagattg cgataccacc acgatgaaaa aagccaatgt
      481 gagctttagc ggtgttggtg atgcagatca atctgatctg atttctgtta gtactgaagc
      541 cggtgcggcg aaaggtgttg gtatcggtat ttatgataac agcaacaccc tggtcgctct
      601 taacggtggt aaagcaagtg ttgatctgag taaaggccag acggttctct actttaccgc
      661 caactacgtt tcaactctgg caacggtaac gaccggttac ggcaatgcgc aggtcgattt
      721 caacctgtct tacgagtaat cgtctgtttt acccatggcg gcgtaattgc cgccattagt
      781 cgtcaaaatt catggatcga atgatgaaaa gtaaatttgt tgcgttggct ctgtctctgt
      841 tcctctccca gtcagttctg gcggggggcg ttgggcttag tgctacccgt gtgatttatg
      901 atggcagtaa gaaagaagcc tcgctgaccg tacagaataa aagtaagacg gaagagtttc
      961 tgatccagtc ctgggtggat gatgccgcgg gcagcaaaaa aacaccgttt ataattaccc
     1021 cgccgttgtt caggctcgat cctggaaaaa acaatatatt gcgtattgta aatactaccc
     1081 ctggcctgcc gcaggatcgc gaatcggtct actgggttaa tgtgaaagcc attccgtcta
     1141 aaagtgacga cagcgaaaac aaaaatgtat tacagattgc tgttcgcacc cgcatcaaac
     1201 tgttttatcg cccggcaggg ttacagggtg atgtaaaaac cgcgccaaac gaactgcgat
     1261 ttacgcgtag cggtaatcag atccgtgtcg ataaccccac cgtttttaat attaccttta
     1321 atcaattttt tgcgaatgac aaagaaattg aaaaagccgg catggtaccc gcaaaagggt
     1381 cattaaatat tacattaccc gccggggcag gtagcgtttc gaagattaaa tataacacta
     1441 tcaatgactt tggatcgtca gcggaaatga tgactaaaaa tgttgattga ttaatacctt
     1501 ttcaggagaa attgctcaat gtcacggaag accgtttcac gtactttttc ttccttttct
     1561 atctctgtgg tggcggttgc tgttgccagc acgttttctg cacatgccgg aaagtttaac
     1621 cccaaattcc tggaggacgt tcagggtgtg ggccagcatg tcgatttaac gatgttcgaa
     1681 aaaggccagg aacagcagtt acccggcatc tatcgtgtct ctgtttacgt caatgaacag
     1741 agaatggaaa cccgcacact ggaatttaaa gaggccacag aagcacagcg caaagcgatg
     1801 ggggaatccc ttgtgccttg ccttagccgg acgcaactgg cagagatggg cgtgcgcgtt
     1861 gaaagctttc cggccctcaa tctggtgcct gccgaagcct gcgtgccgtt tgacgaaatc
     1921 attccccagg cctcaagcca tttcgacttc agtgaacaaa agctggtatt aagtttccct
     1981 caggcggcga tgcaccaggt ggcgcgtggc acggtgccgg aatctctatg ggacgaaggt
     2041 atccctgcgt tgctgctgga ttacagtttt agcgggagca acagtgaata tgactcgacg
     2101 gggtcttcca gcagttatgt cgatgataat ggcactgttc atcatgatga tggtaaagat
     2161 acgctaaaga gcgacagcta ctacctcaac ttacgcagcg ggctgaacct tggcgcttgg
     2221 cggttacgga attacagtac ctggagccat tccggtggca aagcgcagtg ggataatatc
     2281 ggtacgtctt tgagtagggc catcatccct ttcaaagcgc aattaacaat gggggatacg
     2341 gcgaccgcgg gggatatttt tgacagcgtt cagatgcgtg gcgcgatgtt agcctctgat
     2401 gaagagatgc tgcccgacag ccagcgaggc tttgcaccca tcgttcgagg gatcgccaaa
     2461 agcaacgccg aagtcagtat tgaacaaaac ggctacgtta tttatcgcac ctatgtgcag
     2521 cctggtgcct ttgaaataaa cgatctttac cccactgcaa acagcggtga tctcacggtc
     2581 attattaaag aagcggatgg cagtgaacaa cgctttattc agccattctc atcggtgcca
     2641 attttccagc gtgaaggcca tctcaaatat agctttgccg caggtgaata tcaggccggg
     2701 aattatgaca gcgcctcgcc gcgtttcggg cagcttgatc tgatctacgg tttaccgtgg
     2761 gggatgacgg cctacggcgg cgtattaatc tctaataatt acaatgcatt tgcattaggg
     2821 atagggaaaa actttggtta tatcggggcg atttccattg atgtgacgca ggctaaaagc
     2881 gaactgaata acgatcgcga tagccaggga caatcttatc gtttcttata ttccaagagc
     2941 ttcgaaagcg gcaccgattt ccgccttgcg ggctatcggt actctaccag cggtttctat
     3001 accttccagg aagccaccga tgtgcgcagt gacgctgaca gcgactataa ccgttatcac
     3061 aagcgcagcg aaatacaggg taacctgacg cagcaattag gggcctatgg ctctgtttat
     3121 ttaaatttaa cgcagcagga ttactggaac gacgcaggta aacagaacac ggtatcggcg
     3181 ggttacaacg gacgtattgg caaggtcagt tacagtattg catatagctg gaataaaagc
     3241 cctgaatggg atgaaagcga tcgcttgtgg tctttcaata tttccgttcc actaggccgg
     3301 gcctggagta actatcgcgt cacgaccgac caggatggtc gtaccaatca acaggttggg
     3361 gtcagcggaa cgctgcttga ggatcgcaac ctgagctaca gtgtccagga aggctacgcc
     3421 agcaacggtg tgggtaacag cggtaacgct aacgttggct atcagggtgg gtccggtaat
     3481 gtcaacgtag gctatagcta cgggaaagat taccggcagc tcaactacag cgttcgcggc
     3541 ggcgtgatag ttcatagcga aggcgtgacg ctttcccaac cgctaggcga aaccatgacg
     3601 ctcatctccg tacccggtgc gcgcaatgcc cgcgtggtga ataacggcgg cgttcaggtt
     3661 gactggatgg gtaacgcgat cgtgccttac gccatgccgt atcgtgaaaa cgaaatctca
     3721 ctgcgtagcg attcgttggg tgacgatgtt gacgttgaaa atgcgttcca gaaagtggtg
     3781 ccaacgcgtg gagcgattgt cagagcgcgt tttgataccc gcgttggtta ccgcgtatta
     3841 atgacgctgc ttcgttccgc gggcagtccg gtgccctttg gagcaacggc aacgctaatc
     3901 actgataaac aaaacgaggt gagcagtatc gtcggtgagg aagggcagct ctatattagc
     3961 gggatgccag aggaagggcg ggtactgatt aaatggggta atgatgcgtc gcagcaatgc
     4021 gtggctccat ataaattatc cctggaatta aaacagggcg gaattgttcc tgtttcagcc
     4081 aattgccagt aagcaaggag agcgaaatga aagcggcaat agcattatct ttattaggat
     4141 gcgtattcgg attttctggc aaggcattgg ccggaggttc ctgggggcca tgtacccctg
     4201 tggatggcac gacctatcac tataacgtcg atgtggatgt cggtgttccc gatgcaagta
     4261 aaaacgtcgc agggacggtt ttaacggatg tccttaactg gtcgaatgga caaaatgtat
     4321 cgataatttg tgaatgtcct gataagggtg actataaaat tgaaaaagat acgttgatac
     4381 aaggggttag tggaatagcg aatatgacca gggatatcgg tggttttaag tattattact
     4441 taaccgatga actgtctgta tctacaaaaa ttcaggtaag ttcggggcaa tatggttttg
     4501 tcccctttaa aggtcaacag gcgcttcagg taacgggctg taatgaagtc atcactactc
     4561 cttatatggg cggaggagga ttactttctt tcttgattac taaacccttt atcggcgatt
     4621 cagtcattcc ccctacatta attgccgaac tgtatgcctc caaaacaaat aaagattacg
     4681 gaacaatacc catatcttcc gtatctattc agggccgcgt caccataact caggattgcg
     4741 aaataaagcc cggcacggtg ctggatgtgc catttggtga attcccctcc tcagcgttta
     4801 aaaacagaca agggcaaatg cctgaaggtg cgacagagca agaaattaac ctcggctttg
     4861 attgcaacaa tatctcggat ggtatcaaag tctcgttgcg ccttgagggg gcaaccaatg
     4921 ctgacgatcc ccgagcggtg gatatgggca accccgatat tggcgtactg gtgaaagatt
     4981 ccagcggcaa aattttggta ccgaatgatg caagcagcac cacgttatta aatttgagca
     5041 gcctggattc taaaactcac cgcaatgccg cgattcgact gctggcttta ccgattagca
     5101 cgacgggtaa agcgcctaaa ggcgggacgt ttgaaggcgt aacgaccata tatcttgaaa
     5161 tggagtgatg ccatgaaatt caatctattg ctgcatagcg gcatcgccag tttgagtctg
     5221 tttgcctgcg gggtgaatgc ggcgacggat cttggcccgg caggggatat tcatttctcc
     5281 atcactatca ccactaaagc ttgcgagatg gaaaaaagcg atctcgaagt cgatatggga
     5341 acaatgacgc tgcaaaaacc tgcggcagtc ggtacggtgt tgagcaagaa agatttcacc
     5401 attgaactca aagagtgtga tgggatatcc aaagcgaccg ttgagatgga cagtcagtcg
     5461 gacagcgatg atgattccat gtttgccctt gaggctggtg gcgcaacggg ggttgcgttg
     5521 aagatagagg acgataaagg aacgcagcaa gtacccaaag gctccagcgg aacgccgatt
     5581 gaatgggcga ttgatggcga aaccacgtcg cttcactacc aggcgagtta tgtggtcgtc
     5641 aacactcagg ccactggtgg cacagcgaat gcccttgtca atttttccat cacctatgag
     5701 taagttactg ggccagcatc ctggcccaac ctatctctga atagtcgtta atccgttaaa
     5761 atggtctatt ccggacttgc acttttgtaa atttgtgctt catgcacact ctttccccac
     5821 actttttccc tttgctgtgg tctacttatt cgcgcgtgta gattttactt atctgactac
     5881 ctccgcactt ttcccctgct gggcctgaaa agccgctaag cagggtgtta tcacctgttt
     5941 gtccagggtt tgtttgcatg agatacatca aatcgattac acagcagaag ctgagctttt
     6001 tgcttgcaat ctatattggc ctttttatga atggcgcggt tttttaccgc cgcttcggca
     6061 gctatgcgca cgattttacc gtctggaaag gcatttctgc tgttgttgaa ctggccgcca
     6121 ccgtactggt gaccttcttt ttactacgtc ttctttcgct gtttggccgc cgcagctggc
     6181 gtattctggc atcgctggtt gtgctctttt ccgcaggtgc cagctattac atgaccttcc
     6241 ttaatgtggt cattggttat ggcatcatcg cttccgtcat gaccaccgat atcgacctgt
     6301 caaaagaagt tgttggtctg aactttattc tctggttaat cgccgttagc gcattgcctc
     6361 ttatccttat ctggaataac cgctgtcgct acaccttgct ccgacaactg cgaaccccag
     6421 ggcagcgtat tcgcagcctg gcggtcgtcg tactggcggg aattatggtt tgggcaccga
     6481 ttcgtttgct ggatatccag cagaagaaag tggagagggc gaccggcgtt gatttgccga
     6541 gttatggcgg tgtcgtagcg aactcttatc tgccatcaaa ctggctttct gcgttggggc
     6601 tgtacgcctg ggcgcgggtc gatgaatctt ccgataataa ttcattgctt aacccggcga
     6661 agaaattcac ttatcaggca ccgcaaaatg ttaatgacac ttatgtcgtg tttatcatcg
     6721 gtgaaactac gcgttgggac catatgggca ttttcggcta tgagcgtaat accacgccga
     6781 agctggccca ggagaaaaat ctggcggcgt tccgtggtta ctcctgtgat accgcaacca
     6841 aactctctct gcgttgcatg tttgtacgtc aggggggcgc ggaagataat ccgcagcgca
     6901 cattaaaaga acagaacatt ttcgcggtac tgaagcagtt aggattcagt tctgacctct
     6961 acgctatgca gagcgaaatg tggttctaca gcaatacgat ggcggacaat attgcttacc
     7021 gtgaacagat tggtgcggag ccacgtaacc gtggcaagcc ggtagatgat atgttgctgg
     7081 tagacgaaat gcagcaatcg ctggggcgca acccggatgg taagcatcta atcattctgc
     7141 ataccaaagg ttcgcacttt aactacaccc agcgttaccc gcgcagcttt gcgcagtgga
     7201 agccggaatg tattggtgtt gatagcggat gtaccaaagc gcagatgatc aactcctatg
     7261 acaactcggt gacctatgtg gatcacttta tctccagtgt gattgatcag gttcgcgata
     7321 agaaagcgat tgtgttctac gcagctgacc acggcgagtc aattaatgaa cgcgagcacc
     7381 tgcacggcac gccgcgtgaa ctggcaccgc cggagcagtt ccgcgtaccg atgatggtct
     7441 ggatgtcaga taaatatctg gaaaatccgg tcaatgcgca ggcgtttgcg cagctgaaaa
     7501 aagcagccga catgaaagtg ccacgccgtc acgtagagct gtacgacacc atcatgggtt
     7561 gtcttggcta tacttcaccg gatggtggaa ttaacgaaaa caacaactgg tgtcacatcc
     7621 cgcagacaaa agaggcagtg gctaactaag aggcctgctg actttctcgc cgatcaaaag
     7681 gcattttgct attaagggat tgacgagggc gtatctgcgc agtaagatgc gccccgcatt
     7741 cggtgattgg cgcagcctgg tagcgcactt cgttcgggac gaaggggtcg gaggttcgaa
     7801 tcctctatca ccgaccaatt tagaaaaacc agcctacggg ctggtttttt gctttctgca
     7861 acccatgagg atgagaacct ccgggggcag ggaggttcga ctcgacgtag gcctgataag
     7921 acgcgtcagc gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg gcgcgaccgc
     7981 cttattcgac ctacacacca cacatttccc tgccaaattt gcgccccata tctcactatc
     8041 cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa atcttttttt
     8101 gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc atggtattaa
     8161 tcgctcagat atccaccttt tcctcagatt tttttcatta aatgcataaa acttaatcac
     8221 caaatgttgc taaatattaa gctgattgtt ctggtcatgt tggagtagca caaatttctc
     8281 tgctgcaaat agctgtttga agttttttta atctttgttt gtggattctc accgttggtg
     8341 taaatcccgg ttggctgtat tgacgttttc acattctgtt gacagattgt aggtcacgag
     8401 gggcatttta tggaggatcc gcactgttac actgatgtta attagtacgg catccccacc
     8461 tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc aggcagcgac aacccactgc
     8521 aaagggttaa aacaacaaac atcacaattg gagcagaata atgcgtattt ccttgaaaaa
     8581 gtcagggatg ctgaagcttg gtctcagcct ggtggctatg accgtcgcag caagtgttca
     8641 ggctaaaact ctggtttatt gctcagaagg atctccggaa gggtttaacc cgcagctgtt
     8701 tacctccggt accacctatg acgcctcttc cgtaccgctt tataaccgtc tggttgaatt
     8761 taaaatcggc accactgaag tgatcccggg cctcgctgaa aagtgggaag tcagcgaaga
     8821 cggtaaaacc tacaccttcc atctgcgtaa aggtgtgaag tggcacgaca ataaagaatt
     8881 caaaccgacg cgcgaactga acgccgatga cgtggtgttc tcgttcgatc gtcagaaaaa
     8941 cgcgcaaaac ccgtaccata aagtttctgg cggtagctac gaatacttcg aaggcatggg
     9001 cttgccggag ctgatcagcg aagtgaaaaa ggtggacgac aacacagttc agttcgtgct
     9061 gactcgcccg gaagcgccgt tcctcgctga cctggcaatg gacttcgcct ctattctgtc
     9121 aaaagaatat gctgatgcga tgatgaaagc cggtacaccg gaaaaactgg acctcaaccc
     9181 aatcggaacc ggtccgttcc agttacagca gtatcaaaaa gattcccgta tccgctacaa
     9241 agcgtttgat ggctactggg gcaccaaacc gcagatcgat acgctggttt tctctattac
     9301 ccctgacgct tccgtgcgtt acgctaaatt gcagaaaaat gaatgccagg tgatgccgta
     9361 cccgaacccg gcagatatcg cccgcatgaa gcaggataaa tctatcaacc tgatggaaat
     9421 gccggggctg aacgtcggtt atctctcgta taacgtgcag aaaaaaccac tcgatgacgt
     9481 gaaagttcgc caggctctga cctacgcggt gaacaaagac gcgatcatca aagcggttta
     9541 tcagggcgcg ggcgtggcag cgaaaaacct gatcccgcca accatgtggg gctataacga
     9601 tgacgttcag gactacacct acgatcctga aaaagcgaaa gccttgctga aagaagcggg
     9661 tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg gtacaacgtc cgtataaccc
     9721 gaacgctcgc cgcatggcgg agatgattca ggcagactgg gcgaaagtcg gcgtgcaggc
     9781 caaaattgtc acctacgaat ggggtgagta cctcaagcgt gcgaaagatg gcgagcacca
     9841 gacggtaatg atgggctgga ctggcgataa cggggatccg gataacttct tcgccaccct
     9901 gttcagctgc gccgcctctg aacaaggctc caactactca aaatggtgct acaaaccgtt
     9961 tgaagatctg attcaaccgg cgcgtgctac cgacgaccat aacaaacgcg ttgaactgta
    10021 caagcaagcg caggtggtga tgcacgatca ggctccggca ctgatcatcg ctcactccac
    10081 cgtgtttgaa ccggtacgca aagaagttaa aggctatgtg gttgatccat taggcaaaca
    10141 tcacttcgaa aacgtctcta tcgaataatt aaaagccata caagactgat ggcaaaggca
    10201 aaactgcctg atgcgctccg cttatcaggc ctacgaaaat tctgcaatgt attgagtttg
    10261 cacgattttg taggccggat aaggcgttaa cgccgcatcc ggcataaaca aagcgctctt
    10321 tgtcaacaat ctgcataccc ggtggtgctg tgcctctgcg tgcagcgcca tccggcagca
    10381 atacttcatt ccctgcccga cctgggcggg aattgatttg tgagcaatac agacacgcag
    10441 ttccaggctg cgggtcacta cagagaatcc gggttatgtt gcagtttatt ctccgacgtt
    10501 tgggactcgt catccccacg tttatcggta ttacccttct cacatttgcc tttgtccaca
    10561 tgatcccggg cgatccggtg atgatcatgg cgggcgaacg tgggatctcc ccagagcgtc
    10621 acgcgcagtt gctggctgaa ctcggcttag ataaaccgat gtggcagcag tatctccatt
    10681 acatttgggg cgttatgcat ggcgatctag gcatttcaat gaaaagccgc atcccggtat
    10741 gggaagagtt cgtgccgcgc tttcaggcca cgctggaact tggcgtctgc gcgatgattt
    10801 ttgctacggc agtcggtatt ccggttggtg tgctggctgc ggttaaacgc ggttccattt
    10861 tcgatcacac agcggttggc ctggcgctga caggttattc aatgcctatc ttctggtggg
    10921 gcatgatgct gatcatgctg gtttcggtgc actggaacct gacacccgtc tccggtcgcg
    10981 tgagcgatat ggtgttcctc gatgattcca atccgttaac cggttttatg ctgatcgaca
    11041 ccgccatctg gggtgaagac ggtaacttta tcgatgccgt cgcccatatg atcttgcccg
    11101 ccattgtgct gggtactatt ccgctggcgg tcattgtgcg tatgacacgc tcctcgatgc
    11161 tggaagtgct gggtgaagat tacatccgca ccgcgcgcgc caaagggttg acccgcatgc
    11221 gggtgattat cgtccatgcg ctgcgtaacg cgatgctgcc ggtagtgacc gttatcggcc
    11281 tccaggtggg aacattgctg gcgggggcga ttctgaccga aaccatcttc tcgtggcccg
    11341 gtctggggcg ctggttgatt gacgcactgc aacgccgcga ctatccggta gtgcagggcg
    11401 gcgtattgct ggtggcgacg atgattatcc tcgtcaatct gctggtcgat ctgctgtacg
    11461 gcgtggtgaa cccgcgtatt cgtcataaga agtaagggga catcatgtca caggttactg
    11521 aaaataaagt gattagcgca ccggtgccga tgaccccgtt acaggagttc tggcactatt
    11581 ttaaacgcaa caaaggcgcg gtcgtcgggc tggtttacgt cgtcatcgtg ctgtttatcg
    11641 cgatctttgc caactggatt gctccctata acccggcgga acaattccgc gatgcgctgc
    11701 ttgccccgcc cgcctggcag gaaggcggca gcatggcgca cttgctgggc accgatgacg
    11761 taggccgtga tgtgctgtcg cggctgatgt acggcgcgcg tttatcgctg ctggttggct
    11821 gtctggtggt ggtgttatcg ctgattatgg gcgttattct cggcctgatc gccggttact
    11881 ttggcggcct ggtcgataac atcatcatgc gcgtggtcga tatcatgctg gcgctgccaa
    11941 gtctgctgtt ggcactggtg ctggtggcga ttttcggccc gtcgattggt aacgccgcgc
    12001 tggcgctgac ctttgttgcc ttgccgcact atgtgcgctt aacccgcgcc gccgtgctgg
    12061 tggaagttaa ccgcgattac gttaccgcgt ctcgcgtggc aggtgccggg gcgatgcgtc
    12121 agatgtttat taacatcttc ccgaactgcc ttgcgcctct gattgttcag gcgtcgctcg
    12181 gtttctctaa cgccattctc gatatggctg ctcttggttt cctcggcatg ggggcgcagc
    12241 cgccaacacc agagtggggc accatgctct ctgacgtgtt gcagttcgcg caaagcgcct
    12301 ggtgggtcgt gaccttccct ggtctggcga tcctgctgac ggtgctggca tttaacctga
    12361 tgggtgacgg tctgcgtgac gcgctcgatc ccaaactgaa gcagtaagag gttcgagatg
    12421 gcgttattaa atgtagataa attatcggtg catttcggcg acgaaagcgc accgttccgc
    12481 gccgtagacc gcatcagcta cagcgtgaaa cagggcgaag tggtcgggat tgtgggtgag
    12541 tccggctctg gtaagtcggt cagttcactg gcgattatgg ggctgattga ttatccgggc
    12601 cgcgtgatgg cggaaaagct ggagtttaac ggtcaggatt tgcagcgtat ctcagaaaaa
    12661 gagcgccgca atctggtggg tgccgaagtg gcgatgatct tccaggaccc gatgaccagc
    12721 cttaacccgt gctacaccgt gggtttccag attatggaag cgattaaggt gcatcagggc
    12781 ggtaataaga gcactcgccg tcagcgggcg attgacctgc tgaatcaggt cggtattccc
    12841 gatccggcat cgcgtctgga tgtttacccg catcagcttt ccggcggcat gagccagcgc
    12901 gtgatgatcg ccatggcgat tgcctgtcgg ccaaaactgc tgattgccga tgaaccgacc
    12961 actgcgctgg acgtgaccat tcaggcgcaa atcatcgaac tactgctgga gctacagcag
    13021 aaagagaaca tggcgctggt gttaattacc catgacctgg cgctggtggc ggaagcggca
    13081 cataaaatca tcgtgatgta tgccggtcag gtagtggaaa cgggcgatgc gcacgccatc
    13141 ttccatgcgc cgcgtcaccc gtatactcag gcattgctgc gtgcgctgcc ggaatttgct
    13201 caggacaaag aacgtctggc atcgttgcct ggcgttgttc ccggcaagta cgaccgcccg
    13261 aacggctgcc tgcttaaccc gcgctgcccc tatgccactg acagatgtcg cgctgaagaa
    13321 ccggcgctga atatgctcgc tgacgggcgt cagtccaaat gccattaccc acttgatgat
    13381 gccgggaggc cgacactatg agtacgcaag aggccacctc gcaacaaccg ctgttgcagg
    13441 ctatcgacct gaaaaaacat tatccggtga agaaaggtat gttcgctccg gaacgtctgg
    13501 tgaaggcgct ggacggcgtt tcgtttaacc ttgaacgtgg caaaacgctg gcggtagtag
    13561 gtgaatctgg ctgcggtaaa tcgaccctcg gtcggttgct gacgatgatt gaaacgccca
    13621 ccggcggcga gctgtattac caggggcagg atctgcttaa gcacgatccg caggcgcaga
    13681 agctgcgtcg gcagaaaatc cagattgtct tccagaaccc ttacggttcg ctgaatccgc
    13741 gtaaaaaagt tgggcaaatt cttgaagagc cgctgctgat caacaccagc ttaagcaaag
    13801 aacagcgtcg ggaaaaagcc ctgtcgatga tggcgaaagt cggcctgaaa accgagcact
    13861 atgaccgcta tccgcatatg ttctccggcg gacaacgtca gcgtatcgct attgcccgtg
    13921 gtctgatgct cgacccggac gtggtgattg ccgatgaacc ggtctccgcg ctggacgtct
    13981 cggtgcgtgc acaggtgctc aacctgatga tggatttgca gcaggaactg gggctttctt
    14041 atgtcttcat ctcccacgac ctgtcggtgg tggagcacat tgctgatgaa gtgatggtga
    14101 tgtacctggg ccgctgcgtg gagaagggaa cgaaagacca aatcttcaat aacccgcgtc
    14161 atccgtacac tcaggcgcta ctttccgcta caccacgcct gaacccggac gatcgccgcg
    14221 agcgcatcaa actcaccggt gaactgccaa gcccgctgaa tccaccgccg ggctgcgcgt
    14281 ttaacgcccg ctgtcgtcgg cgcttcggcc cctgcaccca gttgcagccg cagctaaaag
    14341 actacggcgg tcaactggta gcttgttttg ctgttgatca ggatgaaaat ccgcagcgtt
    14401 aactcacaaa accggggcca tgcgctccgg tttactcaat cagcttcaac agtggcgaca
    14461 agcatagcaa aatgccgtaa agcagaatca gccaggcttt aaatccgcga agtttttcca
    14521 gttgtgcgac tttatagatc aggaaaaacg gaatgaggca cgacacaatg ccatataacg
    14581 ggcttcccaa ctgaaagaac accagcaccg atacacgaaa cgaaacccaa atcgtcaacg
    14641 taatgacgat aaaagcgcag atcgccagag tcagcatgcg tgagttaatt ttcttggtat
    14701 caataattcg gcttaacaga ttgagaataa tgcctttaat ggcctcgtgg aaaccgagat
    14761 aaatgccaaa gaatgcggtc agtacggcaa agatattcag caccgtagag gtgatatgaa
    14821 tgatatgccc agggatcacc tgcgcggcca gtgccagtgc cgagatattt tgttcaaagg
    14881 cagaaacggc ttcttcgtgg ctaattgaga aggtaaacga aaaggcaaaa aacagtatca
    14941 ccgcgatgag cgtgatataa ctaattcggt gggtacgcag cgcgagccgg gttgccagta
    15001 ctttatccgc ttcccgttta cgataggcaa tattcattgg gttaagtacc tgaataaata
    15061 ctgcagaaaa gaagcaaaat ggaatggtaa gcaggacatc gcggaaaaag acggaggctt
    15121 gcgggaaggc ggttatattg gcgaaattcc agtgcgggat catcgcaaaa ccgaacacga
    15181 caataatccc tactttgacc accaccattg gcccagaaat cttaaacagt aatcgttcac
    15241 caccagacgc aatcgctacc agtacggcga aaatagcgac tttataaagt agagattgtg
    15301 aaagatcggc atcggttaaa ccgaaggttt tcaggtacga ggcgctgtcg aaaaccacgg
    15361 agagagagta gataaaaatc ccgtggataa tcatcaaaaa gtagataacc ccgaggaaaa
    15421 ttccccagtt cttccccagg taatgactga taatatcggt gtagtcatta caggaatcgc
    15481 tttcagaaag ggtttttaaa taaatgtcct gcactaccca ggtggcagga taagcaatga
    15541 tcgctgcggt aataaatacc caaattccct tcaagccaat ttgtactggc atcagcacgg
    15601 ttccggcacc aatcgccatg cctatgcata ataaaaccca gccgaagtcg tagcgtgtaa
    15661 acggcaattt ctgatcgtgt ttcggtagtg tgttgtgctg cataaagtcc tgtccagaaa
    15721 gaagtcggcc tcgcaaaaat gaataaaccc agtttctctt gcgcaacagg tggagaagtc
    15781 gaatacgcag tatgtaaaaa atgaggcaac agattcactg gggaggtcgc gaggaggcgc
    15841 aattctacaa aattgcgcat ttcactacca gtctggagtc agcccggata tgaactgcaa
    15901 ctattgcaaa ttaagaaatt aagctggaag gcatcacgca atgaggaaga ttgccgagtg
    15961 ctgacagtaa aaagcaatcg taaaattagc ggcaagccgg aagggttccg gtgaggcgca
    16021 atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
    16081 ttgcaggtat gcacctgaca acaccacggg ggctaatctt gaacctagac cactcaagaa
    16141 tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca gaactgggca
    16201 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
    16261 tgaactggct gaacaagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
    16321 atttgcgccc ggaccaggcc gcaggggtga aactctgcgg cctttttcgt aagaaaaatg
    16381 caacgattgc aagcggataa tattctgcgg aatgcgcagc acagagtgaa gacgtgacgg
    16441 attccggaac agaaaaacag tcgtagaatc agcgataagc cggaaggatt ccggtgaggc
    16501 gcaatgttgc gggggcttaa tccctggtgg cattggttgc tggagagaga aaacccccgc
    16561 acattgcagg tatgcacctg acaacatcac gggggccatt cttgcatcta gaccattcaa
    16621 gaatagccgc gaaacgttgt cattacaaca caggcggcta tatgacgctc gcagaactgg
    16681 gcatggcctt ctggcacgat ttagcggctc cggtcattgc tggcattctt gccagtatga
    16741 tcgtgaactg gctgaacaag cggaagtaac gtgtcatgcg ggcgtcaggc tgccataatg
    16801 gcaatttgcg cccggaccag gccgcagggg tgaaactctg cggccttttt cgtaagaaaa
    16861 atgcaacggt tgcaagcgga taatattctg cggaatgcgc agcacagagt gaagacgtga
    16921 cggattccgg aaccgaaaaa cagtcgtaga atcagcggca agccggaaag gttccggtga
    16981 ggcgcaatgt tgcgggggct ttatccctgg tggcattggt tgctggagag agaaaacccc
    17041 cgcacgttgc aggtatgcac ctgacaacat cacgggggct aatcttgaac ccaaacaact
    17101 caagaatagc cgcgaaacgt tgtcattaca acacaggcgg ctatatgacg ctcgcagaac
    17161 tgggcatggc cttctggcat gatttagcgg ctccggtcat tgctggcatt cttgccagta
    17221 tgatcgtgaa ctggctgaat aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta
    17281 atggcaattt gcgcccggac caggccgcag gggtgaaact ctgcggcctt tttcgtgctt
    17341 actgcgggta aggcacccag tcgccgccgt tcaggcgaac gtacggttta tcctggtatt
    17401 gaataactac tgcatttgag ttctctgaga ccggtgctgt ttgtggcaac ccactggtga
    17461 gttttttcca gtcaacattg tcttcggtga aaatcttgcc atcaagaacg cgaaccacca
    17521 gatcggagat agccaggaag ctgctcggtt ggtcgatgac aatcggtgcc ccctgatgtg
    17581 gtgccttcat gccgaagaat ttcaccccaa cggggacgtc ggtgatagac gggctaggga
    17641 tatcacgtag gccagatacc tgcattctgt cgcccttcag cgcgccgccg tgttccggca
    17701 ccacgaccac catcacttta cgacccgatt tctccagttc agtaaagaag gcgtccagtt
    17761 catcaaagaa tttctgcgcc cgcgctttgt aatccgctgt tttgctgacg cccggataat
    17821 ggttgccgtc atgcagtgga agcgtgttgt agaacgtggc actacggctg tttttatctt
    17881 tttcggtaac gtccagccag cggttaagca cggcggtatc gtcataaacc ggcgaaccat
    17941 caaagcccag caaaataacc ggcagatttg tttgaaccat caattcagtc tgcatgccgc
    18001 cattttcgcg aacttctttc aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt
    18061 gctgggtaaa gcccagtttc gacaggttat caaacagata gcagtcgtta tttgccggtt
    18121 gatacagatt agtgtgcgaa gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg
    18181 ggccactgta ggaggtggcg gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca
    18241 gtggatgcga catcaaccca gcggcttcta tatccgacca ggaaagcgaa cagatgttaa
    18301 tcaccagtag ttcaaatggc tgagcatcag cgggcagcga agacgggaag gtcgatttac
    18361 gtttcgcctc cgcgttatag aaattattca gccaggcgtt aaggttcgcc gttgttggcg
    18421 gtgcagtttg tgcgggcata tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg
    18481 ccgctgcgtt accacccgtc gttgttacag tggtcgtcgg ttgtccggct ggccacaagg
    18541 agaaacttgg tcccgccagg gtaagtacgt tcagccatag cagtatggca accacaaaaa
    18601 cggtaatgcg aatccattgt gacaggaata accaggccac taataaaaca aaaatggccc
    18661 caatcatctg ccagttaata aagcgtgtga caaggtcgat taaataatcg gtactgaacc
    18721 ccgccacctg cgaaccctgg ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc
    18781 agaacaaagc aaagccgatc ggcagggcaa tccagtggcg caagcgatgc aggctgtagc
    18841 gcggaatggg catcagcaga aacgcggcaa acaccaaatt gaggagcgga tggaagttaa
    18901 gatatcccgc ccacaacagg ccgaacttaa ccagaaaata gaagttccag ccggaaaggc
    18961 cgcgccagta ttgccagagg gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt
    19021 tttggttgcc ctggcttttt ccgtgcggcg taacgtccca gccggtttaa cataacgagg
    19081 tttagcaaag aacaaacgta aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata
    19141 gcccagcggg aaaaatatca gtgcgcaaac gacaataatt tcaatgatat cgctgatggt
    19201 catcatgatg agcgctccac agcatcatct aacagtcgca tgggttctgg tattcgtcgc
    19261 cagtggcgac catcgtgctc ggcattgatg accggtttag aactttgcgt taaaggcagc
    19321 ggcatgcccc attgttctgg ggcaagcagg cgcatcgtcc ggtatcaaca gcctacgtgt
    19381 tggcgacgtt tattacgtag gtcatctgcc gtggttcgag cgcttgtggt atgcgctggc
    19441 aaaccatccg attctgctgg cggtgctggc ggctatcagt gtgatattgc tggcatgggt
    19501 actgtggcgt ctgctgcgaa ttattagtcg tcgtcgtctt aacccggata acgagtaatt
    19561 gaagatgaat gtgttgcgta gtggactcgt gacgatgctg ctgttggctg cctttagtat
    19621 tcaggcagcc tgtacctggc ctgcctggga gcagtttaaa aaggattaca tcagtcagga
    19681 agggcgcgtc atcgacccca gcgacgcgcg caaaatcacc acctctgaag ggcaaagtta
    19741 cggcatgttc tttgccctgt cggctaacga ccgtgcagct ttcgataata ttctcgactg
    19801 gacgcagaac aatctcgctc agggttcttt aaaagaacgt ttgcccgcct ggctgtgggg
    19861 caagaaagag aacagtaagt gggaagtgct ggacagcaat tcggcctccg atggtgatgt
    19921 ctggatggcc tggtcgttgc tggaggcggg gcgtttgtgg aaagagcagc gttataccga
    19981 catcggtagc gcgttgctaa aacgtatcgc gcgggaggaa gtggtgacgg tgcctgggct
    20041 gggttccatg ttgttaccgg gcaaagtggg ttttgctgag gataacagct ggcgttttaa
    20101 ccccagctac ctgccgccga cgctggcgca gtatttcacc cgctttggtg cgccgtggac
    20161 tacgctgcgc gaaaccaatc aacgtttatt gctggaaacc gccccgaaag gcttttcgcc
    20221 agactgggtg cgctatgaga aagacaaagg ctggcagcta aaagccgaaa aaacattgat
    20281 cagcagctac gacgccatcc gcgtttacat gtgggtaggc atgatgcctg acagcgatcc
    20341 gcaaaaagcg cggatgctca accgatttaa accgatggcg acattcactg agaaaaacgg
    20401 ttatccgccg gaaaaagtgg atgtggctac ggggaaagcg cagggcaaag gaccggtcgg
    20461 tttttctgcc gccatgctgc cctttttaca aaaccgcgat gcgcaggccg ttcagcgcca
    20521 gcgcgtggcc gataactttc ccggcagcga tgcctattac aactatgtgc tgaccctgtt
    20581 tggacaaggc tgggatcaac accgtttccg cttctcgaca aaaggtgagt tattacctga
    20641 ctggggccag gaatgcgcaa attcacacta aacatattca cgctttccct cggtctggcc
    20701 gtcatgccga tggtcgaggc agcaccaacc gctcagcaac agttgctgga gcaagttcgg
    20761 ttaggcgaag cgacccatcg tgaagacctg gtgcaacagt cattgtatcg gctggaactt
    20821 attgatccga ataacccgga cgtcgttgcc gcccgtttcc gttctttgtt acgtcagggc
    20881 gatattgatg gcgcgcaaaa acagctcgat cggctgtcgc agttagcgcc gagttcaaat
    20941 gcgtataaat cgtcgcggac tacgatgcta ctttccacgc cggatggtcg tcaggcactg
    21001 caacaggcaa gattgcaggc gactaccggt catgcggaag aagctgtggc gagttacaac
    21061 aagctgttca acggtgcgcc gccggaaggt gacattgctg tcgagtactg gagtacggtg
    21121 gcgaaaattc cggctcgccg tggcgaagcg attaatcagt taaaacgcat caatgcggat
    21181 gcaccgggca atacgggcct gcaaaacaat ctggcgctat tgctgtttag tagcgatcgc
    21241 cgtgacgaag gttttgccgt cctggaacag atggcgaaat cgaacgccgg gcgcgaaggg
    21301 gcctctaaaa tctggtacgg gcagattaaa gacatgcccg tcagcgatgc cagcgtgcag
    21361 gcgctgaaaa aatatctttc gatctttagt gatggcgata gcgtggcggc tgcgcaatcg
    21421 caactggcag aacagcaaaa acagctggcc gatcctgctt tccgcgctcg tgcgcaaggt
    21481 ttagcggcgg tggactctgg tatggcgggt aaagccattc ccgaactaca acaggcagtg
    21541 cgggcgaacc cgaaagacag tgaggctctg ggggcgctgg gccaggcata ttcacagaaa
    21601 ggcgatcgcg ccaatgcagt ggcgaatctg gaaaaagccc tcgcactgga cccgcacagc
    21661 agcaacaacg acaaatggaa cagtctgctg aaagtaaacc gctactggct ggcaatccag
    21721 cagggcgatg ctgcgctgaa aaccaataat cctgaccggg cagaacgcct gttccagcag
    21781 gcgcgtaatg tcgataacac cgacagttat gcagtgctgg ggctgggcga tgtggcgatg
    21841 gcgcgcaaag attaccccgc cgctgaacgc tattatcagc agaccctgcg gatggacagc
    21901 ggcaacacta acgccgtgcg cgggctggcg aatatttatc gccagcagtc gccagaaaag
    21961 gctgaagcgt ttatcgcctc gctctctgcc agccagcggc gcagcattga tgatatcgaa
    22021 cgcagcctgc aaaacgaccg tctggcacag caggctgagg cactggaaaa ccagggcaaa
    22081 tgggcgcagg cggcagcact tcagcggcaa cgactggcgc tggaccccgg cagcgtatgg
    22141 attacttacc gactttcgca ggatctctgg caggccggac aacgcagcca ggccgatacg
    22201 ttaatgcgca atctggcgca gcagaagccg aacgacccgg agcaggttta cgcttacggg
    22261 ctgtacctct ctggtcatga ccaggacaga gcggcgctgg cgcatatcaa cagcctgccg
    22321 cgtgcgcagt ggagcagcaa tattcaggag ctggttaatc gactgcaaag cgatcaggtg
    22381 ctggaaaccg ctaatcgcct gcgagaaagc ggcaaagaag ctgaagcgga agcgatgctg
    22441 cgtcagcaac caccttccac gcgtattgac ctcacgctgg ctgactgggc gcagcagcga
    22501 cgtgattaca ccgccgcccg cgctgcatat cagaatgtcc tgacgcggga gccaactaac
    22561 gccgatgcca ttcttggtct gacggaagtg gatattgctg ctggtgacaa agcggcggca
    22621 cgtagccagc tggcgaaact gcctgccacc gataacgcct cgctgaacac acagcggcgc
    22681 gtggcgctgg cgcaggcgca gcttggagat accgcagcag cgcagcagac gtttaataag
    22741 ttgatcccgc aggcaaaatc tcagccaccg tcgatggaaa gcgcgatggt gctgcgcgac
    22801 ggtgcgaagt ttgaagcgca ggcgggcgat ccaaagcagg cgctggaaac ctacaaagac
    22861 gccatggtcg catccggtgt gactacgacg cgtccgcagg ataacgacac ctttacccga
    22921 ctgacccgta acgacgagaa agatgactgg ctgaaacgcg gcgtgcgcag cgatgcggct
    22981 gacctctatc gccagcagga tcttaacgtc accctcgagc acgattactg gggttcgagc
    23041 ggcaccggtg gttactccga tctgaaagcg cacactacca tgttgcaggt ggatgcgccg
    23101 tattctgacg ggcggatgtt ctttcgcagt gatttcgtca atatgaacgt cggcagtttc
    23161 tccactaatg ccgatggcaa atgggatgac aactggggca cctgtacatt acaggactgt
    23221 agcggcaacc gcagccagtc ggattccggt gccagcgtgg cggtcggctg gcgaaatgac
    23281 gtctggagct gggatatcgg caccacgccg atgggcttca acgtggtgga tgtggtcggc
    23341 ggcatcagtt acagcgatga tatcgggccg ctgggttaca ccgttaacgc ccaccgtcgt
    23401 cccatctcca gttctttgct ggcctttggt gggcaaaaag actccccgag caataccggg
    23461 aaaaaatggg gcggcgtgcg tgccgacggc gtggggctaa gcctgagcta cgataaaggt
    23521 gaagcaaacg gcgtctgggc atcgcttagt ggcgaccagt taaccggtaa aaatgtcgaa
    23581 gataactggc gcgtgcgctg gatgacgggc tattactata aggtcattaa ccagaacaat
    23641 cgccgcgtca caatcggcct gaacaacatg atctggcatt acgacaaaga tctgagtggc
    23701 tactcactcg gtcagggcgg ttactacagt ccgcaggaat acctgtcgtt tgccataccg
    23761 gtgatgtggc gggagcgcac ggaaaactgg tcgtgggagc tgggggcgtc tggctcgtgg
    23821 tcgcattcac gcaccaaaac catgccgcgt tatccgctga tgaatctgat cccgaccgac
    23881 tggcaggaag aagctgcgcg gcaatccaac gatggcggca gcagtcaggg cttcggctat
    23941 acggcgcggg cattacttga acgacgtgtt acttccaact ggtttgtcgg cacggcaatt
    24001 gatatccagc aggcgaaaga ttacgccccc agccatttcc tgctctacgt acgttattcc
    24061 gccgccggat ggcagggtga catggattta ccgccgcagc cgctgatacc ttacgccgac
    24121 tggtaagttt tcagatagcg cctctcttaa tgccgctgcg atcgggtata ctcgggcggc
    24181 aatctgggat ttccgggggg agacaatttg cgcgtaagtc gctcgttaac aatcaagcag
    24241 atggcaatgg tggcagccgt tgtcctggtg ttcgttttta ttttttgcac cgttttgctg
    24301 ttccatctgg ttcagcagaa tcgctataac acggctacgc aactggaaag cattgctcgc
    24361 tctgtccgcg aacctttatc ttcagccatt ttgaaaggtg atattcccga agcggaagct
    24421 attcttgcca gcattaaacc ggcaggcgtg gtcagccgtg ccgatgtagt gctgcctaac
    24481 cagttccagg cgctgcgtaa aagttttatt ccagagcgtc cggtgccggt aatggttact
    24541 cgcctgtttg agctaccggt tcaaatctcg ctgggcgtct actcgctcga acgtccggca
    24601 aatccgcagc caattgccta tctggtgcta caggcggatt ccttccgtat gtataagttc
    24661 gtgatgagca ccctctcaac gttagtgacc atttacttac ttttgtcgtt aatattgacg
    24721 gtggcgatta gctggtgcat taaccgcctg attttgcatc cgttacgcaa tattgctcgc
    24781 gaacttaacg ccatcccagc ccaggagctt gttggtcacc aactggcatt accgcgtctg
    24841 catcaggacg atgaaatcgg tatgttggtg cgcagttaca acctcaacca gcaattgctg
    24901 cagcgccatt atgaagaaca gaacgaaaat gcgatgcgct tcccggtgtc ggatttgccg
    24961 aacaaagcct tgctgatgga gatgctggag caggttgtcg cgcgtaaaca aaccaccgcg
    25021 ctgatgatca tcacctgtga aaccctgcgt gatactgcgg gtgtgctgca agaggcgcaa
    25081 cgagaaattc tgctgctgac gctggtggaa aaactcaaat cggtactgtc gccacgtatg
    25141 atcctcgcgc agattagcgg ttatgacttt gctgtcattg ccaacggtgt acaggaaccg
    25201 tggcacgcaa tcactttggg tcagcaagtg ctcactatca tgagcgagcg cctgccgatt
    25261 gaacgtattc aactccgtcc gcactgtagc attggcgtgg cgatgttcta cggcgatctc
    25321 accgccgaac agctttacag tcgcgctatt tctgcggcat ttaccgctcg ccataaaggc
    25381 aagaatcaga ttcagttctt tgatccgcag cagatggaag ctgcccagaa gcggttgacg
    25441 gaagagagcg atatccttaa tgcactggaa aatcatcagt ttgctatttg gttacagcca
    25501 caggtcgaga tgaccagcgg taaactggtc agtgcggaag tgttactgcg tatccagcaa
    25561 ccggatggca gttgggacct gccggatggc ttaatcgatc gcattgagtg ctgtgggctg
    25621 atggttaccg tcggtcactg ggtgctggaa gagtcctgtc gactgttggc tgcctggcaa
    25681 gagcgcggca ttatgctgcc cttgtcggta aacctctctg cgctgcaact gatgcacccg
    25741 aatatggtgg cggatatgct ggaactgtta acccgctatc gcattcagcc gggaacactg
    25801 attctggaag tgacagaaag ccgacgtatt gacgaccctc atgctgcggt ggcaatcctc
    25861 cgtccgctgc gcaatgccgg agttcgggtg gcgctggatg atttcggcat gggctacgca
    25921 gggctgcgtc agctgcagca tatgaaatcg ttgccaatcg acgtactgaa aatcgacaaa
    25981 atgtttgttg aaggcttgcc ggaagatagc agcatgattg ctgcaattat catgctggcg
    26041 cagagcctga acttacaaat gattgccgaa ggcgtggaga ctgaagcaca acgcgactgg
    26101 ctggcaaaag cgggcgttgg tattgcccag ggcttccttt ttgctcgtcc actccctatt
    26161 gaaatcttcg aagagagtta cctggaagaa aagtagctac cccaaactga ttacaaaact
    26221 ttaaaaagtg ctggtttgtg cgagccagct caaacttttt aacctttttg tttcaattat
    26281 gatccaggta catttctgtg atgttgtctg ggtgttattt taaggccgca ggtaccccat
    26341 aaccttacaa gacctgtggt tttactaaag gacaccctat gaaaacctct ctgtttaaaa
    26401 gcctttactt tcaggtcctg acagcgatag ccattggtat tctacttggc catttctatc
    26461 ctgaaatagg cgagcaaatg aaaccgcttg gcgacggctt cgttaagctc attaagatga
    26521 tcatcgctcc tgtcatcttt tgtaccgtcg taacgggcat tgcgggcatg gaaagcatga
    26581 aggcggtcgg tcgtaccggc gcagtcgcac tgctttactt tgaaattgtc agtaccatcg
    26641 cgctgattat tggtcttatc atcgttaacg tcgtgcagcc tggtgccgga atgaacgtcg
    26701 atccggcaac gcttgatgcg aaagcggtag cggtttacgc cgatcaggcg aaagaccagg
    26761 gcattgtcgc cttcattatg gatgtcatcc cggcgagcgt cattggcgca tttgccagcg
    26821 gtaacattct gcaggtgctg ctgtttgccg tactgtttgg ttttgcgctc caccgtctgg
    26881 gcagcaaagg ccaactgatt tttaacgtca tcgaaagttt ctcgcaggtc atcttcggta
    26941 tcatcaatat gatcatgcgt ctggcaccta ttggtgcgtt cggggcaatg gcgtttacca
    27001 tcggtaaata cggcgtcggc acactggtgc aactggggca gctgattatc tgtttctaca
    27061 ttacctgtat cctgtttgtg gtgctggtat tgggttcaat cgctaaagcg actggtttca
    27121 gtatcttcaa atttatccgc tacatccgtg aagaactgct gattgtactg gggacttcat
    27181 cttccgagtc ggcgctgccg cgtatgctcg acaagatgga gaaactcggc tgccgtaaat
    27241 cggtggtggg gctggtcatc ccgacaggct actcgtttaa ccttgatggc acatcgatat
    27301 acctgacaat ggcggcggtg tttatcgccc aggccactaa cagtcagatg gatatcgtcc
    27361 accaaatcac gctgttaatc gtgttgctgc tttcttctaa aggggcggca ggggtaacgg
    27421 gtagtggctt tatcgtgctg gcggcgacgc tctctgcggt gggccatttg ccggtagcgg
    27481 gtctggcgct gatcctcggt atcgaccgct ttatgtcaga agctcgtgcg ctgactaacc
    27541 tggtcggtaa cggcgtagcg accattgtcg ttgctaagtg ggtgaaagaa ctggaccaca
    27601 aaaaactgga cgatgtgctg aataatcgtg cgccggatgg caaaacgcac gaattatcct
    27661 cttaatctca tcacttatgc ccgcactccc ttccccgagt gcgggtgttt gcgcataatt
    27721 gacccctgtg cctgaactta ccacctgggt gagttcaggt ttatttcact tcttccgtga
    27781 cattttcatg ttcttgcggt ctaacacgaa gtgtttttaa cgtcatttag actgaccgac
    27841 aacgggcagt ttttttatta ccaggatagt tgatcagggg ttcacatgca gggcacaaaa
    27901 attcgacttt tagcgggcgg tttgctgatg atggccactg ctggctatgt gcaggcagat
    27961 gcgctccagc ctgatccagc atggcaacag gggacgcttt ccaacggttt acagtggcaa
    28021 gtgctgacca ccccccagcg tcccagcgat cgtgttgaaa ttcgcctgct ggttaatacc
    28081 ggttcgctcg ccgaaagtac acaacagagc ggttacagtc acgccatccc tcgtattgcg
    28141 ctaacgcaaa gcggtggcct tgacgcagca caggcgcgtt cattgtggca gcaggggatc
    28201 gaccctaaac gcccgatgcc gccggtaatt gtctcttatg acaccacgct gtttaatctg
    28261 agtttgccca ataaccgtaa cgatttgctg aaagaggcgc tctcttatct ggcaaatgcc
    28321 actggcaaac tgaccatcac accagaaacc atcaaccacg cgctgcaaag tcaggacatg
    28381 gtggcaacct ggcctgccga tactaaagag ggctggtggc gctatcgtct gaaagggtca
    28441 accttgttag gtcacgatcc tgccgatccg ctgaaacaac ccgttgaagc ggaaaagatt
    28501 aaagatttct atcagaaatg gtacaccccg gatgcaatga cgctgctggt ggtgggaaac
    28561 gtggatgcgc gctcggttgt cgaccaaatc aacaaaacgt ttggcgaact gaaaggcaaa
    28621 cgtgaaacac cggctccggt gccgacgctt tctccgctgc gtgcggaagc ggtgagtatt
    28681 atgactgacg cggtgcgtca ggaccggtta tctatcatgt gggatacgcc gtggcagccg
    28741 attcgtgaat cagccgcact gctgcgctac tggcgtgcgg acctggcccg tgaggcgctg
    28801 ttctggcatg ttcagcaagc gttaagcgcc agtaacagca aagacatcgg tcttggattt
    28861 gactgccgtg tgctgtatct gcgtgcgcag tgtgccatca acatcgaatc accaaacgac
    28921 aagctgaaca gcaaccttaa tctggtggcg cgtgaactgg cgaaggttcg cgataaaggt
    28981 ctgccggaag aagagttcaa tgcgttagtg gcgcaaaaga aactggagct gcagaaactg
    29041 tttgccgcct atgcacgggc tgataccgat attctgatgg gccagcggat gcgttcgttg
    29101 caaaatcagg ttgtcgatat cgcgccggag cagtatcaga aactgcggca ggatttcctt
    29161 aacagcctga ctgtggagat gttaaatcag gatctgcgtc agcagttgtc gaatgatatg
    29221 gcgttaatac tgctgcagcc gaaaggcgag ccggaattta acatgaaagc gttgcaggcg
    29281 gcctgggatc aaatcatggc cccatctacc gccgctgcga ccacctctgt cgccacggat
    29341 gacgtacatc ctgaagtgac ggatattcca cctgcacagt aatgcctgat ggtgatgagt
    29401 tagcacaacc atcaggtatt tatggatgag ctggatagag gggtaacact ttatccagcc
    29461 ttttgcatat gctgcgttta cgctggcatc gcctcacgcg ggataatcgc gccgcgatac
    29521 tgaataacgg tacttgcggt caggtgcccg cgtttcgctg cgttttccgc actgccgccc
    29581 gtcaaacgta ccgccagata accggcactg aaagagtcgc cagctgcggt ggtatcgatc
    29641 actttttctt tcggcagttt caccgccgga acatccacta acccttcgcc agcaatggac
    29701 accaggcaag aatccgcccc gcgtttcacc accacttctt tcacgcccgc gttatgggtg
    29761 cgcgcaatga cgtcttccac cggctgttga ccccacaacg cgtcttcgtc gtccagcgtc
    29821 aggaaggcga tatccgtgca ttccagcatt tgctggtaca cctgctgtgt ctcttctttg
    29881 ctggcccaca ggcgcggacg atagttattg tcgaaaatca cttttccgcc gttggcgcgg
    29941 cattcgcgca gcagggaaag cagtttctcg cggctggtcg ggcttaagat cgccagacta
    30001 atcccgctca ggtagagata atcgaaattc gccagctctt cgcaaatcgc cgcagactgc
    30061 tcgctctcca gccagaattt ggcggcggct tcgttacgcc agtagtagaa cgtacgctcg
    30121 ccggtgctgt cggtttcaat gtagtaaagg cccggcagac ggttttccat ccgttgggtc
    30181 agggaagtat caacgttctc gccgtgccag gcgtccagca tttgctggct aaaactgtcc
    30241 gttcccagcg ccgttacgta atgaacggtt aatgccgcag gatcgacctg acgggcgata
    30301 tagacggaag tgttcagggt atcgccgccg aaaccgcgct taacgtccgc gcctttctcg
    30361 gaaagctcaa tcatgcattc gccaatcacg gcaatctttt tggacatagt cgtgaacctg
    30421 atctgtaaaa aattagcgtt agtttgcgct gtggtggttt gctggtcaac catattaaaa
    30481 cagtgttcca ttattttttt gagccagaac gtattcctga aagattccgt tgtggagtga
    30541 ggaaatttac cccggccagg cggtaaagtt ctgcccttac gcgccgataa tctttgtcga
    30601 gtccgggcag catcactttt aaacacagga catctttgat gataaggcag gttatccagc
    30661 gaataagcaa ccctgaagca agcatcgaga gcttgcagga acggcgtttt tggttgcagt
    30721 gtgagcgtgc ttacacctgg cagccgatct atcaaacatg cgggcggtta atggccgtgg
    30781 agctattaac ggtggtcacg catcccttga acccttcgca gcgcctgccg ccggatcgct
    30841 attttactga aatcaccgtc agccatcgga tggaggttgt gaaagagcag attgatttgc
    30901 tggcgcaaaa agccgacttc tttatagagc acggcctgct ggcatcggtc aatattgatg
    30961 gccctacgct catcgccctg cgtcagcaac caaaaatcct gcgccagatt gagcgtcttc
    31021 cctggctgcg tttcgaactg gtggagcata tccgtctgcc gaaagattca acctttgcct
    31081 cgatgtgtga atttggcccg ctgtggctgg atgattttgg taccgggatg gcaaatttct
    31141 ctgcactaag tgaagtgcgt tatgactaca tcaaaatcgc gcgagaactg tttgtgatgc
    31201 tgcgtcagtc gccggaaggc cgcacactct tttctcagct tttacatcta atgaatcgct
    31261 attgtcgcgg ggtgattgtc gagggcgtag aaacaccgga agagtggcgt gatgttcaga
    31321 actcgcccgc attcgccgca caaggctggt ttctttcacg cccggcaccg atagaaacgc
    31381 tgaatacggc ggttctggcg ctataagctg cctcattttc cgcctggctg gactatcttt
    31441 aggactggca caggaagaat atgaggaagc gaacgatgag caaggcaggc aaaataaccg
    31501 ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc gatcattttg attgcaactt
    31561 ttgactggaa tcgactcaaa ccgaccatca accagaaagt ctctgcggag ttgaatcgtc
    31621 cgttcgctat ccgtggcgat ctgggcgtgg tgtgggagcg gcaaaagcaa gaaactggct
    31681 ggcgcagctg ggtgccgtgg ccccatgtac acgcggaaga catcattctt ggcaatccac
    31741 cggatattcc cgaagtcacg atggtgcatt tgccacgcgt agaggcaacg ctggcaccgt
    31801 tggcgctgct gaccaaaacg gtctggctac cgtggatcaa actggaaaag cccgacgcac
    31861 gcctgattcg cctctctgaa aagaacaata actggacgtt taatcttgcc aacgatgaca
    31921 acaaaaacgc gaatgcaaag ccgtcggcgt ggtcgtttcg gctggataat attctgttcg
    31981 atcaagggcg gatcgccatt gatgacaaag taagcaaagc ggatctggaa atttttgtcg
    32041 atccgttagg caagccactg ccgttcagcg aagttactgg atcgaaaggt aaagcggata
    32101 aagaaaaggt gggcgattac gtttttggcc tgaaggcgca gggacgttat aacggtgaac
    32161 cgctcactgg tacgggaaaa ataggcggta tgctgacgct gcgtggcgaa gggacgccgt
    32221 ttccggtaca ggctgatttc cgttcaggta atacccgtgt tgcttttgat ggcgtcgtga
    32281 atgacccaat gaagatgggc ggtgtcgatt tacggcttaa attttctggc gattcgctgg
    32341 gtgatctcta tgaactgacg ggcgttctgc tgcccgatac cccgccgttt gaaacggatg
    32401 gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt ctttgattat cgcggcttta
    32461 atgggcgcat tggcgatagc gatatccacg gttctctggt ctacaccacc ggcaaaccac
    32521 gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg gctggcggac ctgggaccgt
    32581 tgattggcgt tgattccggg aaaggggcag aaaagtcgaa acggtctgaa cagaagaagg
    32641 gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta tgaccgcttc gaaaccgata
    32701 aatgggacgt tatggatgcc gatgttcgct tcaaagggcg gcgcattgag catggcagta
    32761 gcctgccgat tatcgatctt tctactcata tcatcctcaa aaatgctgac ctgcgcctgc
    32821 aaccgctgaa atttggcatg gcgggcggca gcattgcggc gaatattcat ctggaaggcg
    32881 ataaaaagcc gatgcagggg cgggcagata ttcaggctcg tcgactgaaa ctgaaagaac
    32941 tgatgcccga tgtggaactg atgcagaaga cgctggggga aatgaacggt gacgcggaac
    33001 tacgcggtag cggtaactcg gtggcggcac ttttaggcaa cagtaacggc aacctgaaac
    33061 tgttgatgaa tgacgggctg gtgagccgca acctgatgga gattgttggg ctgaatgtcg
    33121 gcaactacat tgtcggtgcg atatttggtg atgatgaggt gcgggtgaac tgcgcggcgg
    33181 cgaatctgga tattgccaac ggcgtggcgc gcccgcagat ctttgctttc gatactgaga
    33241 acgcgttgat taacgttacc ggcacggcaa gttttgcttc ggaacagctg gatttgacta
    33301 ttgatccgga gagtaaagga attcggatta tcacactgcg ttcgccgctg tatgtgcgtg
    33361 ggacgtttaa aaatcctcag gctggggtga aagccgggcc gttgattgcc cgtggtgctg
    33421 ttgctgcggc actggcaacg ctggtaacac cggcggcggc gttactggcg ctgatctcac
    33481 cttccgaagg ggaggctaat cagtgtcgga cgattttgtc gcagatgaag aagtgatgat
    33541 aattgccgga tgtctgacat ccggcaatta aacagattta gattgttggc aaagtgtgct
    33601 ttgttcatgc cggatgcggc gttaacgcct tatccggcct acaaatcctt gtgaattcaa
    33661 tatattgtat ggaaattcag gcctgataag cgtagcgcat caggcttttc acacttatta
    33721 caacgactga tgtcgcgtct catgggtcag cagcagggcg attaacgtca agccagccat
    33781 cgccgccaga tataacccca ccgcacctaa cccgtagtta gtctgcaacc aggctgcgat
    33841 atacggcgca acggaagccc cgagaatcga cgctacgttg taagagaatg atgctccggt
    33901 gtaacgcact tctgttggga acagctctgg taacagcgcg cccatcggcc cgaaggtcag
    33961 acccatcaga cttaacccca gcagcaggaa ggcaaaaacc agaatcgggt tgccagaacc
    34021 gagcagtggg ttaaaggcga acagcgcgaa caggatgatc agcgtggtga taattaccat
    34081 gcttttacgg cgaccaaagg catcagccag taatccggcg actggcacca tcacgccaaa
    34141 accaataact gccatcatca gcatccacaa cacttcgtta cgcggcaggc caagcccaac
    34201 tggcgcggcg gcggtactga aggtcatcga gtagaccgtc atgatgtaaa acagcgtata
    34261 ggttgccagc ataatgaacg tacccagtac ggttacgcga acatgtttgg tcagcagcgt
    34321 acccagcggg atcttcacct gttttttcgc tttagcgact ttctcaaaca ccggcgactc
    34381 atgcagcgac acgcgaacat acaggccgat aatgaccagc accgccgaga agataaatgg
    34441 cacacgccag ccccagctca taaactgctc atcggtcagt agccaggaaa gcagcaagaa
    34501 agtgccgttg gcaaagaaga agccaatcgg tgcgcccagc tgtgggaagg agccatacag
    34561 tgcacgtttg cgcggtgggg cgttttcagt cgccagcagc gccgcgccgc cccattcacc
    34621 gcctaagccc agaccctgac caaatcgagc cagcgccagc agcagcgggg cgaaaatacc
    34681 aatcgtggca tagcccggca gcagaccaat caccacggtc gaaatcccca tcgttagcaa
    34741 cgaggcgacc agcgtcgctt tacgcccaac gcgatcgcca aaatgaccaa aaacggcaga
    34801 gccaatgggg cgcgcgacga aggcgatggc gaaggtggcg agcgactgta gcgttgctgc
    34861 cgttggatcg ccctgcggga agaagatatg cggaaacaca ataacggccg cagtggcgta
    34921 aatgtaaaag tcgaagaact caatggccgt gccaatgaga gaggcgacaa ggactttatt
    34981 acgcgagttg atcggcgtgt attcttgctc gtggtcgagt gttgtggctg ttgcttgcat
    35041 aatcttttct tattttttgg ctaacgaata gccatattac gcacagcaaa agtgatattt
    35101 caatgtgtaa caaatcgcgg aaatggtcaa aaataaggca aaaaggggat aatgtttagg
    35161 ccagtgattt ttcttctatg aaatgcttca cataatttta taaatggtgg ataaaactgg
    35221 tttcaggtta aataaaagtt accaactgga tttatatgct ggggttatga gttggcatga
    35281 aattgtttgc tcccaaatag atggctgaag aggctctggg gaagagtttc aagaggttat
    35341 gtgcgcatta tagccggatg cgatgctggc gcatcttatc cggcctacgg ggagccaggc
    35401 cgaataaatt cgtacgccgc atccggccta ttttaaggct gcgttttccc cggcattcgc
    35461 gggtcgtctt tatattcggc ggtggcaatc cacgccgcgc aaaacagcgt caaacgggcg
    35521 aagaagtaga aaaatgccat cagccccagc acggaaccaa atgctgcacc agagggggat
    35581 ttcatcaacg atggcagggt gtaggtcatc acgattttaa tcacttcaaa accaatagca
    35641 gccagaaatg ttccgcgaat cagggctttt ttgcgcggac ggtggcgcgg caaccgccag
    35701 aagatccaga agaaaagcag atagttggct aagatggaaa tcgccaggcc aatcaaccgc
    35761 cacgtcggtt tcagccactc aatgctgttc aggtgcaggg cgctaataat catttgctgc
    35821 gccgaaccgg caaccgaggt gatcgaaagc gtcacaatca gcgcaatcaa caaaccaatc
    35881 agcgaaataa aatcacgcag atattttacc cagaacttct cctgatcttg cggcgagcgt
    35941 tcccagacat cgcgcgactg ggcgcgaatc gcttcacgca ggttacccat ccagttgatg
    36001 ccggaataaa gcgccaccgc caggccgaca agccctacag tcgtacgctg ctgaacggcg
    36061 gtgttgatgg tgtttttcaa cgtagcggct agcgtcggat cgctgatgtt ttgcagaatt
    36121 ttgtcgaaga tatcctgtag caacatcgga tgggaggcca gcacaaagcc ccctgcggca
    36181 aacgacacca tcaaaatcgg gatcatcgac aaaaatgaga aataggtgat agccgcacca
    36241 aactggttac ccagccgatc gttaaagcgt tctgtcgcgc gaataaggtg cgcaataatc
    36301 ggttggcgtt ggactttttc ggcagtggtg gtgacggttt ccagcgcctg gctaactttg
    36361 ccgcctttct cgctattatc caacggctta atcggctcgt gctccagatc ctgggtggga
    36421 cgtttgatct cgttttcctg cgtcatccgc tcttttgtcc ttctgtcgtg gtcattctta
    36481 aaaagtatag tcggtcagtc caggtactct ttcattacgc cagccagcca ctgcataaac
    36541 aggtttacac gccgggaaag ctcccgacgc tgcggataaa ccagcgaaag ggagagcggc
    36601 tcggcacggt agcctggcaa cacttcaata agccgcccgg cacgcagggc ttcgcgcacg
    36661 gcaatgcgcg ggatctgaat aatccccaga ccggcaagac cggcggcgag atagttttcg
    36721 ctactgttta ccgtcaacat gccgccagac ttaaaccact ggacgccatt aacgctggca
    36781 acctcaaaac ctaacggatg tacacccagg tgcggtgtat aacgcactat tgcgtgtgaa
    36841 gtcagatcat cggggctttg cggataacca aagcgcgtca gatagtgcgg actggcacag
    36901 ttgaccatgg tcagtttgcc gaggggacgg gcgataacgc catcttccgg taacgcgcca
    36961 gtgcgtatca cgcaatcaaa accatcatga agaatatcta ccggacggtc atggctactc
    37021 agttccagct caattcccgg atgcagatag agaaattccg acaggcgcgg cagtaacagg
    37081 cttttcgcga ttcctggcgg gatgtcgatg cgtaatttac cgctgatact ggtggcatcc
    37141 tgttgaaaca gaccgtccag ttcgctgaga ttactcaaca catcttttgc ccgttgataa
    37201 taggtcatgc cttctggcgt gagtttgacc cgtcgcgtgg tgcgctggag aagctgggtg
    37261 ccaagttgat gttccagtgc ctgtatctgg cgcgaaacac ttccctttgg caaagcaaag
    37321 aaatccgccg cgcgggaaaa actttccagc tccgcgactt taatgaacaa ctgcattgcg
    37381 tgaattttat ccatttcttt gaccgattgt tgttcacagc gaaacagtaa cgcacatatc
    37441 gcattgttta ttgttttttc taacatctaa tgccgggttt cacaatgttg tcataacgaa
    37501 tgtggcgatt tctcagcagg aaaagggcgg aatagctttc tgagaaaggt gaatcccctt
    37561 aaacagagcg tatttttcag cgattccctt taaagtgaat agaggatgta ttggctattt
    37621 gtgcttattt ttgtctcatt gccgtttgtt attttggctt gtggtcgcgg aatttcattt
    37681 ttaattgcat gcgtactaac caccaatata aggaaatcct caaaattgtt ttaaatagtg
    37741 ccctcgacat gtaatttcag gcgtttagct gtaactattt atatcggtaa ctattttcta
    37801 cgcgtcatct ctgtagaatg gccaccgcca aatggcgaat tcttattttt tctcgcgggt
    37861 aatacccttc cactacttaa gtctttttaa catattcagg tggagattca gttatgcaaa
    37921 tagtcatgtt tgacaggcag tcaatattta ttcatggaat gaaaatcagt ttacagcagc
    37981 gtattccagg agtgagtatt cagggggcca gtcaggcaga cgagttatgg caaaagctgg
    38041 aaagttaccc tgaagcctta gttatgctcg atggcgatca ggatggtgag ttttgctact
    38101 ggttgctgca aaaaaccgtg gtgcaatttc ctgaggttaa ggtgttaatt acggcgacgg
    38161 attgcaataa acggtggtta caggaagtta tccattttaa tgtgctggcc attgtgcctc
    38221 gtgattcaac cgtcgagaca tttgcgttgg cggtaaatag tgcggcgatg gggatgatgt
    38281 ttctgcctgg agactggcgg acgacgccgg aaaaggacat taaagatctc aaatcgttaa
    38341 gcgcccgcca gcgggagatt ttaaccatgt tagcggcggg cgaatcaaat aaggagatcg
    38401 gcagagcgct gaatatcagt accggaacgg taaaagcgca tctggaatcc ctgtatcgcc
    38461 gtctggaagt gaaaaatcgc acccaggcgg caatgatgtt aaatatctcc tcctgaagag
    38521 gataatttta cagccagcag gaagttagcg gctggctgta aaaatattat ggttcgccgt
    38581 acaaaccaat taaacggcgt accacaccgt tagtccagcc aaacccatcc tgcaacggat
    38641 actcgccacc gccgccttcg cggggaacac catcggcaat atggtatttt tcgatcagtt
    38701 tgtgctgttc cagatagaac tgattcaccg tcttcagcca gcttcgcgcg atttcatcac
    38761 ccagaaggtc atcgccatac attttaaatc cctgaattgc catccactgt aacggtgccc
    38821 agccgttggg tttatcccac tgttcaccgg tttcgtactc gcttgccaga atcccgccag
    38881 gtgtcagtaa ccgactgcgc acggcgtttg ccagacgatc ggcctgttca tggttcgcca
    38941 taccgacata gagtggcaca atggcggcag cggaaaacag cgccagttgt tcgcgtcgcc
    39001 agtcgtaatc gcggtagatg ccgttttcat catcccagag gtaacggttt accgcatcgc
    39061 gacgggcact ggccttctgg cggaacagtg cttctgtctc tttctcacct ttcagcgccg
    39121 agatgttggc gatggcgctc tccagtttaa acaggaaggc attcagatcg atggggatga
    39181 actgggtggt acgaatgctc gccagacgac cagtatcacg cagccaacgg gaagagtaat
    39241 cccaaccgga ggccgccccc gcgcgtaaat cgcggtacac ctcgttgggc gggcgaccag
    39301 aatgtttcgc ggtttcaacg tcctcaagcc aggattcgtc acgcggcgtg tcgcgatcgt
    39361 cccagtaacg gttgagcagc gatccgtccg gcatccgcac aacatggcga taggcctgat
    39421 tagggatcaa cgattctgca ccgtccatcc agaaggcata ttccattttc aggtggtcga
    39481 gatagcggcg tgcaccgcgt acaccatctt cttcaaacaa ctccaccatc agcgcaaaaa
    39541 ccggtggttg cgagcggctc aaataatagg tgcggttgcc gttggggatg tgaccgtagt
    39601 tttcgatcat ccaggcgaag ttatcggcca tgcatttcag caaatcttcc cgaccacttt
    39661 ccgccagccc cagcatggtg aagtaggaat cccaatagta ggtttcgcta aaacggccgc
    39721 ccgggacaat atatgactgc ggcagcgcca gcagagaaga ccacggaatg tgatcctgtg
    39781 gttcgcgggt tagcaccggc cacaactggt cgatatgctc tttcagggaa ttttgcgggt
    39841 ccgatacata ctcgctggag tagacctccg gcagccagaa gtgattttca acaaacttgc
    39901 gcaagtcaaa atcacgatga cggcgcactt tacggtagcg gattaaaata tccagcgggt
    39961 ccattttggg tgcgcagtcg ggaaaggttt tactgtcggg gaaaattttc gccgactgaa
    40021 catgctcgaa cagttcgaga tagcgatcgg caggcgtcag cgcatcagag gcgggcagcc
    40081 cttcaatcat ctcgggttcc ggttctgcct cgatcatctc atccagtttt aattcatacg
    40141 ggtccagttc atagcagaga tcgacctcga tcatcagttc gtctggatta gggttttgaa
    40201 ttttctgatt gagcataagg atccaacctc cgaaattcgt cgctaattat cggcttttct
    40261 ttaagggtag tcattcgctc cgcctggtgg gcgacaaagc gtgcggtgga tcacgtttat
    40321 taacctgtca
//
LOCUS       DAPGWD010000028        38380 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPGWD010000028 DAPGWD010000000
VERSION     DAPGWD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.028"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            167..1825
                     /locus_tag="RHX88_001398"
     CDS             167..1825
                     /locus_tag="RHX88_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDV2230108.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1822..2778)
                     /locus_tag="RHX88_001399"
     CDS             complement(1822..2778)
                     /locus_tag="RHX88_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDV2230109.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2929..4044
                     /gene="macA"
                     /locus_tag="RHX88_001400"
     CDS             2929..4044
                     /gene="macA"
                     /locus_tag="RHX88_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDV2230110.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4041..5987
                     /gene="macB"
                     /locus_tag="RHX88_001401"
     CDS             4041..5987
                     /gene="macB"
                     /locus_tag="RHX88_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDV2230111.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6060..6284)
                     /gene="cspD"
                     /locus_tag="RHX88_001402"
     CDS             complement(6060..6284)
                     /gene="cspD"
                     /locus_tag="RHX88_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDV2230112.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6607..6927
                     /gene="clpS"
                     /locus_tag="RHX88_001403"
     CDS             6607..6927
                     /gene="clpS"
                     /locus_tag="RHX88_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDV2230113.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6958..9234
                     /gene="clpA"
                     /locus_tag="RHX88_001404"
     CDS             6958..9234
                     /gene="clpA"
                     /locus_tag="RHX88_001404"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDV2230114.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9578..9665)
                     /locus_tag="RHX88_001405"
     tRNA            complement(9578..9665)
                     /locus_tag="RHX88_001405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9629..9631),aa:Ser,seq:gga)
     gene            complement(9919..10137)
                     /gene="infA"
                     /locus_tag="RHX88_001406"
     CDS             complement(9919..10137)
                     /gene="infA"
                     /locus_tag="RHX88_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDV2230115.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10422..11126)
                     /gene="aat"
                     /locus_tag="RHX88_001407"
     CDS             complement(10422..11126)
                     /gene="aat"
                     /locus_tag="RHX88_001407"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDV2230116.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11168..12889)
                     /gene="cydC"
                     /locus_tag="RHX88_001408"
     CDS             complement(11168..12889)
                     /gene="cydC"
                     /locus_tag="RHX88_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDV2230117.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12890..14656)
                     /gene="cydD"
                     /locus_tag="RHX88_001409"
     CDS             complement(12890..14656)
                     /gene="cydD"
                     /locus_tag="RHX88_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDV2230118.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14779..15744)
                     /gene="trxB"
                     /locus_tag="RHX88_001410"
     CDS             complement(14779..15744)
                     /gene="trxB"
                     /locus_tag="RHX88_001410"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDV2230119.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16289..16783
                     /gene="lrp"
                     /locus_tag="RHX88_001411"
     CDS             16289..16783
                     /gene="lrp"
                     /locus_tag="RHX88_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDV2230120.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16918..21024
                     /gene="ftsK"
                     /locus_tag="RHX88_001412"
     CDS             16918..21024
                     /gene="ftsK"
                     /locus_tag="RHX88_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDV2230121.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVERQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQPQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21179..21790
                     /gene="lolA"
                     /locus_tag="RHX88_001413"
     CDS             21179..21790
                     /gene="lolA"
                     /locus_tag="RHX88_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDV2230122.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21801..23144
                     /gene="rarA"
                     /locus_tag="RHX88_001414"
                     /gene_synonym="mgsA"
     CDS             21801..23144
                     /gene="rarA"
                     /locus_tag="RHX88_001414"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDV2230123.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23235..24527
                     /gene="serS"
                     /locus_tag="RHX88_001415"
     CDS             23235..24527
                     /gene="serS"
                     /locus_tag="RHX88_001415"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDV2230124.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24766..27210
                     /gene="dmsA"
                     /locus_tag="RHX88_001416"
     CDS             24766..27210
                     /gene="dmsA"
                     /locus_tag="RHX88_001416"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDV2230125.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27221..27838
                     /gene="dmsB"
                     /locus_tag="RHX88_001417"
     CDS             27221..27838
                     /gene="dmsB"
                     /locus_tag="RHX88_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV2230126.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27840..28703
                     /gene="dmsC"
                     /locus_tag="RHX88_001418"
     CDS             27840..28703
                     /gene="dmsC"
                     /locus_tag="RHX88_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDV2230127.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28739..29365)
                     /locus_tag="RHX88_001419"
     CDS             complement(28739..29365)
                     /locus_tag="RHX88_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV2230128.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29680..30828
                     /locus_tag="RHX88_001420"
     CDS             29680..30828
                     /locus_tag="RHX88_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2230129.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31038..32468
                     /locus_tag="RHX88_001421"
     CDS             31038..32468
                     /locus_tag="RHX88_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2230130.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32469..33377)
                     /locus_tag="RHX88_001422"
     CDS             complement(32469..33377)
                     /locus_tag="RHX88_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2230131.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            33477..34067
                     /locus_tag="RHX88_001423"
     CDS             33477..34067
                     /locus_tag="RHX88_001423"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HDV2230132.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(34149..34889)
                     /gene="pflA"
                     /locus_tag="RHX88_001424"
     CDS             complement(34149..34889)
                     /gene="pflA"
                     /locus_tag="RHX88_001424"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDV2230133.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(35081..37363)
                     /gene="pflB"
                     /locus_tag="RHX88_001425"
     CDS             complement(35081..37363)
                     /gene="pflB"
                     /locus_tag="RHX88_001425"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV2230134.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(37418..38275)
                     /gene="focA"
                     /locus_tag="RHX88_001426"
     CDS             complement(37418..38275)
                     /gene="focA"
                     /locus_tag="RHX88_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDV2230135.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9457 a         9356 c        10205 g         9362 t
ORIGIN      
        1 gattttgttg tttttgcaga cttatcagca agagggagta taacgcgatt attcgctcat
       61 ttttcagaca tttgccatgc ttaaatgtga tgtcatcacg tattagcaag gcctttcccg
      121 ttatactgcc agcgtaaagg ataagtcaca tatttctgga ggggatatga ttcttgagcg
      181 cgttgaaatt gtgggttttc gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa
      241 cgtcctgatt ggggagaacg cgtggggtaa atccagcttg ctggacgcct taaccctgct
      301 gctatcgcca gaatcagatc tctaccattt tgagcgcgac gatttctggt tcccgccggg
      361 agatatcaac gggcgagaac atcatctgca tattattttg accttccgcg aatcgctgcc
      421 aggccgacat cgggttcgcc gttatcggcc gctggaagcg tgctggacgc catgcaccga
      481 tggctatcac cgtatttttt atcgtctgga aggggagagt gcggaagacg gcagcgtgat
      541 gacactgcgc agttttctcg ataaagacgg acatccgatt gatgtcgagg atattaacga
      601 tcaggcacgc catctggtgc gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat
      661 gcgccgtatt cgtaacggca cggtgccaaa tgttcccaat gtggaagtca ccgcgcgcca
      721 gctcgatttc ctcgcccgtg agttgtcctc acatccgcaa aatctctctg atggacagat
      781 tcgtcaggga ctttccgcaa tggtgcagtt gcttgagcat tatttctctg agcagggggc
      841 tggacaggcg cgatatcgtt taatgcggcg gcgggccagc aatgagcaac gaagctggcg
      901 ctatctggac atcatcaacc ggatgattga ccgacctgga gggcgctcgt atcgggttat
      961 tttgctcggc ctgtttgcta ctttattgca ggcaaaaggc acattgagac tggataaaga
     1021 cgcgcgtccg ttattgctaa ttgaagatcc agaaacccgc ctgcatccga ttatgctttc
     1081 cgttgcctgg catctgttga atcttttgcc tttacagcgt attgctacca ccaactctgg
     1141 cgagctgctt tcgttaactc cagtggaaca tgtttgccgc ctggtgcgtg aatcttctcg
     1201 tgttgccgcc tggcgtctgg ggccaagtgg cttgagcacc gaagacagcc gccgcatctc
     1261 ttttcacatt cgttttaatc gtccgtcatc gctgtttgct cgctgctggt tgctggtgga
     1321 aggggaaacg gaaacctggg ttatcaatga actggcgcgt cagtgcggac atcattttga
     1381 tgccgaaggg atcaaggtca ttgagtttgc ccagtccggg ctaaagccac tggttaaatt
     1441 tgcccgccga atggggattg aatggcatgt actggtcgat ggcgatgaag cagggaagaa
     1501 atatgccgct acggtacgca gcctgttgaa taacgatcgg gaagccgaac gagaacattt
     1561 aacggcgtta ccggcgctgg atatggaaca ttttatgtat cgccagggat tttccgatgt
     1621 gttccaccgc gtggcgcaaa tcccggaaaa tgtaccgatg aatctgcgca aaattatctc
     1681 gaaagcgatc catcgctctt ccaaacccga tcttgccatt gaagtggcaa tggaggccgg
     1741 acgtcgtggt gtggactccg taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg
     1801 gctggcgcgc ggtcgcgcgg attaaccgcg aaacatcgtg gccatttgtg gctgaatagc
     1861 gtcgagcatc tcatagcgcc gacggtattc agcccgtttt ttactggcga tttcggcaat
     1921 ctcttttcgt gctatctgtg ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc
     1981 aaccgattcc cagaaagcgt tgtaatcggc atggatcttg ccttctttat cacgataacg
     2041 caggctgcgg taaatatgcg tttcattgct gacggcaata atctgctcta cctgcaaacg
     2101 ttgggcaaac agacaggccg cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc
     2161 tttcgtcgca ttctggattt cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc
     2221 aataaacatc gttcttttcc cctgatattc acacagggta aacgtgatct ctgccagagg
     2281 aataccttcg ctgttgcgaa acaggattgt gctgtcacct tctttatcca ttgagatcat
     2341 catggtcagc tcaagcgtga actgctcgcc gtttttgcct tccagtttag ccagttgcag
     2401 cccgggggta ttcaaatata agctgaattc ttccgccgac atacatccgc ggagtaacgc
     2461 ataatggtaa cgtaacgcct ccagcaattg cttacggcta agattcgccg caaggtaagg
     2521 gcgatgcaga cgcacaggca gtcgcggttg gcgcgttaac aacacattga gattaggcca
     2581 gtgggaaagt tcgttcatcc actcaacgct taaacgcggc ataatcaacg agcgcagcaa
     2641 aaatttctgg cgaaaactac ggcgatgcca gaatttaccc ggccgacact gtccacgtgc
     2701 cagactaaga aaaagtgaca ggctgctgag agattcagat ggcgtaaagg tccgttcagt
     2761 tagctgcgac atattcatga aatcaatggt tatacatgac gtcgatttca ccattgcgta
     2821 tcttaaccaa acatcaatag tgtgattact aacgtaaatt ttagggtttt gttgatattt
     2881 cgttgaagtt aatgacccgg attggcatat ggagtattca gaatatttat gaaaaagcgg
     2941 aaaaccgtga agaagcgtta cgttattgcg ctggtgatag tcatcgccgg actgattacg
     3001 ttgtggagaa ttcttaacgc acccgtgccg acttatcaga cactgattgt gcgccccggt
     3061 gatttacagc aaagcgtgct ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg
     3121 ggcgcgcagg tcagcggtca gttgaaaact ctgtcggtgg cgattggcga taaagtaaaa
     3181 aaagaccagc ttttaggggt tattgatcct gaacaggctg aaaaccagat taaggaggtc
     3241 gaagcaacgc tgatggagct acgtgcgcag cggcagcagg cggaagcgga gctgaaactg
     3301 gcgcgggtga cgtattcccg tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag
     3361 gatctcgaca ccgccgcgac ggagatggct gtgaaacagg cgcaaattgg caccattgac
     3421 gcgcaaatca agcgcaatca ggcctctctc gatacggcta aaaccaatct cgattacact
     3481 cgcatcgttg ccccgatggc cggggaagtc acgcaaatca ccactctgca aggccagacg
     3541 gtgattgccg cacaacaagc accgaacatt ctgacgctgg cagatatgag caccatgctg
     3601 gtaaaagcgc aggtttctga agcggatgta atccacctga agccggggca aaaagcctgg
     3661 tttacggtac ttggcgatcc actgacgcgc tacgaggggc aaatcaagga tgtactaccg
     3721 acgccggaaa aggttaacga cgctattttc tattacgccc gttttgaagt ccccaacccc
     3781 aatggtttgc tgcggctgga tatgactgcg caagtgcata ttcagctcac cgatgtgaaa
     3841 aatgtgctga cgatccctct gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa
     3901 gtcaaattgt tgcgtaatgg tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac
     3961 gataccgatg ttgagattgt caaagggctt gaagcgggcg atgaagtggt gattggtgag
     4021 gccaaaccag gagctgcaca atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct
     4081 atcctgccgg tgatgagcag gttgaggtgc tgaagggcat caccctcgat atttatgccg
     4141 gtgagatggt agcgattgtt ggcgcttcag gttccggtaa atcgaccctg atgaatattc
     4201 tcggctgtct ggataaggcc accagcggca cctatcgcgt cgccggtcag gatgttgcca
     4261 cactggacgc cgatgcgctg gcgcaactgc gccgcgagca tttcggcttt attttccagc
     4321 gttaccattt actttcgcat ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg
     4381 ccggtcttga gcggaaacag cggctgcttc gcgcccaggg gttactgcaa cgactggggc
     4441 tggaagaccg tacagagtat tatccggcac agctttcggg tggtcagcaa cagcgcgtca
     4501 gcatcgcgcg ggcattgatg aacggcggtc aggtaattct tgccgatgaa ccaaccggcg
     4561 cactggacag ccattctggc gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg
     4621 ggcatacggt gattatcgtc acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga
     4681 tcgaaattcg cgacggcgaa attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg
     4741 ctggcgggac ggagcctgtt gtcaacacgg tgtctggctg gcggcagttt gtcagcggtt
     4801 ttaacgaggc gctgacgatg gcatggcggg cgctggcagc gaataaaatg cgtactttac
     4861 tgaccatgct ggggattatt atcggtattg cgtcggtggt ttccattgtc gtggtgggtg
     4921 acgccgccaa acaaatggtg ctggcggata ttcgttctat tggtacgaat actattgatg
     4981 tctatcccgg gaaagatttt ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg
     5041 acgacttaat cgccatccaa aaacaaccgt gggtcgcctc agccacacct gccgtctcgc
     5101 aaaacctgcg cctgcgttat aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg
     5161 gcgattattt taatgtctat ggcatgacct tcagtgaagg aaacaccttt aatcaggagc
     5221 agctgaacgg tcgtgcgcag gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc
     5281 cccataaagc agatgtggtt ggcgaggtga ttctggtcgg caatatgccc gccagagtca
     5341 ttggtgtggc ggaagaaaaa cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc
     5401 taccttacag cacgatgtcc gggcgagtta tgggccagtc gtggctgaac tccattactg
     5461 tcagggtaaa agaaggattt gacagcgccg aggcggaaca gcaactcacg cgtttacttt
     5521 cactgcgcca cggaaagaag gatttcttta cctggaacat ggacggcgtc ttgaaaactg
     5581 ttgaaaagac cacacgtact ttacaactgt ttctgacgct ggtggcggtg atttcgctgg
     5641 tggtgggcgg tattggtgta atgaatatta tgctggtgtc agtgaccgag cggacgcggg
     5701 aaattggcat tcgcatggct gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga
     5761 tcgaagccgt actggtttgc ctggtcggtg gcgcgttggg aataacactg tcactgttaa
     5821 ttgctttcac cttgcagctt ttcttacccg gctgggagat tggtttttca ccgttggcac
     5881 tgctgctggc gtttctctgc tcgacggtca ccgggatttt atttggatgg ttacccgcac
     5941 gaaatgcggc acgactggat ccagtagatg ctctggcacg agagtaattt ttgagataaa
     6001 aatgccagcc gatcgggctg gcattttgcc tttaggatgt acacaatgag acagaagagc
     6061 tatgcgactg ccgcttctac ttcgacgggc acaataacac tggcgtgatt gccttttggc
     6121 ccctggtgga catcaaactg aacggattgt ccagctttta gcgttctgta accatccatc
     6181 tgaatggtgg aataatgagc gaaaatatct tcgccgccgc cttcagggca gatgaaacca
     6241 aaccctttgg cattgttgaa ccacttaaca gtaccctttt ccatgcttcg acatccttcg
     6301 caaatcttat acaagtaaga tggaataaac cggggtcaga gagggggctg ttcaaaacct
     6361 cgccaactct agaaatacaa tttagagaat tagggcgagc cgtcaagcgt ttgacagggg
     6421 ccagggggca ggtatgaatc aaaaatttga agcagttaac gctattgaca ggaatgtgac
     6481 agatgtcgct gatgccaacg atagatgata gttatctatc atgtggagta gattggtcag
     6541 gcaaataagc tcttgtcagc ggcagggcgt tctgccgata accgtaaccg aagatgataa
     6601 ctgacaatgg gtaaaacgaa cgactggctg gactttgatc aactggcgga agaaaaagtt
     6661 cgcgacgcgc taaaaccgcc atctatgtat aaagtgatat tagtcaatga tgattacact
     6721 ccgatggagt ttgttattga cgtgttacaa aaattctttt cttatgatgt agaacgtgca
     6781 acgcaattga tgctcgctgt tcactaccag gggaaggcca tttgcggagt ctttaccgcc
     6841 gaggttgcag aaaccaaagt ggcgatggtg aacaagtacg cgagggagaa tgagcatcca
     6901 ttgctgtgta cgctagaaaa agcctgaatg caggcataaa aattggggga ggtgcctatg
     6961 ctcaatcaag aactggaact cagtttaaat atggctttcg ccagagcgcg cgagcaccgt
     7021 catgagttta tgaccgtcga gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg
     7081 gaggcgctgg aagcgtgttc tgtggatttg gttgcgctcc gtcaggaact ggaagccttt
     7141 attgaacaaa ccacacccgt tctgcctgcc agtgaagagg agcgcgacac acagccgacg
     7201 ctgagttttc agcgtgtact gcaacgtgcg gtcttccatg tccaatcctc cggtcgcaat
     7261 gaggtaaccg gtgcaaacgt tctggtcgct atctttagcg aacaggagtc gcaggcggca
     7321 tatctgttgc gtaaacatga agtcagccgt ctcgatgtgg tgaactttat ctctcatggc
     7381 acgcgtaaag acgagccgac acagtcttct gatcctggca gccagccaaa cagcgaagaa
     7441 caagctggtg gggaggaacg tatggagaat ttcacgacga acctgaatca gcttgcgcgc
     7501 gtgggcggaa tcgacccact gattggtcgt gagaaggagc tggagcgtgc tattcaggtt
     7561 ctctgccgtc gccgtaaaaa caacccgctg ctggtggggg aatctggtgt cggtaaaacc
     7621 gcgattgcgg aaggtcttgc ctggcgaatt gttcagggcg atgtgccgga agtgatggct
     7681 gactgtacga tttactctct cgatatcggt tctctgttag cgggcactaa atatcgcggc
     7741 gactttgaaa aacgttttaa agcgttgctc aagcagctgg agcaggacac taacagcatc
     7801 ctgtttattg atgagatcca caccattatc ggtgcggggg cagcgtctgg tggccaggtc
     7861 gatgcggcta acctgatcaa accgttgctc tccagcggta aaattcgcgt aattggttcg
     7921 acaacctatc aggagttcag caacattttc gagaaagacc gtgctctggc gcgtcgcttc
     7981 cagaaaattg atattactga accgtcgatc gaagaaactg ttcaaatcat caatggcctg
     8041 aaaccgaagt atgaagcgca ccacgacgtg cgttataccg caaaagcggt gcgtgcagcg
     8101 gtagagctgg cggtgaaata cattaacgat cgtcatctgc cggataaagc cattgacgtt
     8161 atcgacgaag cgggcgctcg cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt
     8221 aatgtggcgg atattgagtc cgtggtggcc cgtattgcgc gcattccaga gaagagtgtt
     8281 tctcagagtg accgcgatac cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc
     8341 ggtcaggata aagccattga ggcgctgact gaagccatta agatggcgcg tgcaggttta
     8401 ggtcacgaac ataaaccggt tggttcgttc ctgtttgccg gccctaccgg ggtcgggaaa
     8461 acagaggtga cggtacagct ttcgaaagcg ttgggcattg agctgctgcg ctttgatatg
     8521 tccgagtata tggaacgcca taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt
     8581 ggttttgatc agggaggttt gctgactgat gcggtcatca agcatccaca tgcggtgctg
     8641 ttgctggacg aaatcgagaa agcgcatccg gacgtgttca atattctgtt gcaggtgatg
     8701 gacaacggta cgctgaccga taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg
     8761 gtgatgacca ccaacgccgg ggtacgtgaa actgagcgta aatccattgg tcttatccac
     8821 caggataaca gcaccgatgc gatggaggag atcaagaaga tctttacacc ggaattccgt
     8881 aaccgtctcg acaacattat ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg
     8941 gtggataaat tcatcgtcga gttgcaggtt cagctggatc agaaaggtgt ttctctggaa
     9001 gtgagccagg aagcgcgtaa ctggctggcc gagaaaggtt acgaccgggc aatgggcgca
     9061 cgtccgatgg cgcgtgtcat ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg
     9121 tttggttcgc tggtggacgg cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag
     9181 ctgacttacg gattccagag tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat
     9241 tgttaggtag gttggtcaag tccgtaatct cgaaagaggt tacggacttt ttgtttatgg
     9301 ggtggaggag gttcagaccc tttttttaat gatgatggta agttgttgat aattagtgct
     9361 gcgggaaggt aaggataaaa aagggtgctg caggagaatg ggatggtttt gctttattaa
     9421 caacgggcta aacgtgtagt atttgagttc actgccgtac aggcagctta gaaatcgacg
     9481 gggtgcggta aaacctttgc gaacgcgttc actgccgtac agacagataa aatgcgaaaa
     9541 aaaagctcgc actttcgtac gagctcttct ttaaagatgg cggtgagggg gggattcgaa
     9601 cccccgatac gttgccgtat acacactttc caggcgtgct ccttcagcca ctcggacacc
     9661 tcaccaaatt gttttgtcgc ctgacctcat gggtggcaac ggggcgctac tatagggagt
     9721 tggagtaaaa cggtcaagaa gaattttaat gataattatt gtttgctcat actgtaaaca
     9781 agttgtgcag tatatctaca tcgagacaag ttacggactt atacttccaa agtacttcat
     9841 acatatcaca aaataaaaag gccggttaaa ccgacctttt actcgttctt tctcttcgcc
     9901 catcaggcgg taaaacaatc agcgactacg gaagacaatg cggcctttgc tcaggtcgta
     9961 cggggtcagt tcaacagtca ctttgtcgcc cgtcaggatg cggatgtagt ttttgcgcat
    10021 tttaccggag atgtgtgcag taaccacgtg accgttttct aactctacgc ggaacatggt
    10081 attaggcaac gtttcaagaa cggtaccttg catttcaata ttgtcttctt tggccatcta
    10141 atcctctggg gtatcactac cgtaatttga accggcaaga taatgccgaa gttctgtaaa
    10201 taagtaaaga tttgcgcgct aaatcgcaac aaacaggttc ggcacattac tccgaaaaca
    10261 cacggctaag ccgcaccaaa agcgcaacgt ataagggagc ggtgagataa acgatgggcg
    10321 ttacctgacg cgaaaaattc cttatcggca gcggggtaat gagcgtaacc aactctgcga
    10381 ccgcaattat aacactctgg ggagaaatgt gccgaaaaca ttcattcttg tggtgaaaac
    10441 aagcatcgtg gtacccagaa attattcggc aatcgtccga ggcgcatttg attgagataa
    10501 ttaaggtaat cccggcgggg aatttcgcag gcaccaagcg atgctgtgtg atcgttaagg
    10561 acctggcagt cgataagctt accgccatga ccgataaatt cctcacagaa taccagaagc
    10621 gccgttttag acgcattttc catccggctg aacatggact cgccacaaaa tagcgttccc
    10681 tgggccacgc cgtacatacc gccgacaagc tcatcttcac gccagacttc aatggagtgg
    10741 gcatggccga gttcgtgaag gcgatggtag gcttcgacca cgccacgcgt gatccaggtt
    10801 ccttcttcgc gatcgctggc acagccttca atgacctgac caaaagcgta attcatcgtg
    10861 acacgatagg gcgagcgttt atgaaatcgc ttcatactac ggctgatatg cagtgattct
    10921 ggccatagca ccgcgcgggg atcgggcgac caccagagga tggggtcgcc tggagaaaac
    10981 cacggaaaaa taccacgctg gtaagccatt aacaggcgcg cagggctaag atcgccccca
    11041 agtgccagca ggccgttagg ctcacgtaat gcgccttccg gggaagggaa ggctattgaa
    11101 tggcgagaaa gctgaaccag gcgcatgacc gcaaaactcc acgcaagtcg gatcgttcaa
    11161 taatagctta caaaccctgc ttgaactggt aataacgccc ctgtctggca agcagctctg
    11221 cgtgagtacc ttgctcaata atttgcccgt tgtccatcac tattatttgt tggaaacgag
    11281 agagtccgcg aagtcgatgg gtgaccatta acaccgtttt ctcacgcatc atttctgcaa
    11341 gcaattcaag gatctggctt tcggttgtgg catctaagcc ttcggtaggt tcatccagca
    11401 acaccagtgg cgcatcatgt aacagcgcac gggcgatagc cagacggcgc agttcaccac
    11461 cggagagctg gcgtccgcct tcacctaacc aactgttgag acctgcatcc tcgagcagct
    11521 tctccaggcc aacgcgacgc aagctctccg ccagagcctc atcactactg ccaggcgagg
    11581 cgagtaacag attatcacgc agcgtggcgc taaacagatg cactcgctga ggaacaacgc
    11641 tgatggtctg tcgtagagcc gcttcattca ggctggctat ggggctatcg ttaagcaaaa
    11701 tctcgccctg ttgcgggtcc caagcgcggg tcagcagttg taacagtgtt gatttgccgc
    11761 atccggttcg cccgagaatc gcaatatgtt ccccggcgtt tacctgaaga gaaatccctt
    11821 taagtgcctg ttgggattgc tccggataag tgaactgaac atcccgtaat gtcagcgaaa
    11881 cgcgatcggc aacacgagtt tgggtatcag ggaaggtgac ttccggtttt tgatccgtta
    11941 aatcagtgat ccgaacggca gaggcaatga cttgccccag atgctgaaat gcacccgtta
    12001 ctggtgccag tgcttcaaac gcggctaacg cgcagaagac aaacagggca attaacgcgc
    12061 cgggttgagc attgccgcca acgccgccag acgccatcca cagcatcagg atcaccgcta
    12121 acgcgccaat gagtagcatt atcgcttgcg acaatgcggt cagttcagat tgacggcgtt
    12181 gcgcttccag ccattgaatt tctgtattct ctagttgcgt gcgataacga tcgctggcac
    12241 caaaaatggt cagctcagct tgcccttgca gccaggccgt cagttgttgg cgatactgtc
    12301 cgcgaagatg agtcagattt tgcccggtgc tttttcccgc acgataaaac agcggtggca
    12361 tcaggaaaag cgtcagtaac ataatgccgc ccagcgtaaa ggcgagggtg aaatcaagga
    12421 aacttaaccc gattgtcacc accataatca ccacaaaagc gcccaccagc ggcgagataa
    12481 cgcgcagata aagatgatcg agcgtatcaa catccgccac cacgcgattg agcaattcgc
    12541 cctgacgata gcgcgccagt ccggcagggg agaggggcag caatttgctg aaggtgtaaa
    12601 tgcgcagatg ctgcaacacg cggaaagtcg cgtcgtgact taccagacgt tcaaaataac
    12661 gcccggcagt acgggtgatt gctgcgccac gcacgcccgc agcgggtagc atatagttga
    12721 agctgtacag tccggcaacc cccgcaaccg ctgaggccga gaggaaccag ccggaaagtg
    12781 tcaacagacc gatactggcg agcagcgtga caattgccag cacgatacca agacttaaca
    12841 tccatttatg acgtttatac agtgccagat agggtagcaa agcgcgcatt taaatctcct
    12901 cctgacgatg ggccagtaat gtggcgaatg ggccaccagc cacacttaat tccgcgtaac
    12961 gtccttgctc aataatccgg ccatcctgca taacccaaat gacatcccag tcagcaagat
    13021 cttctaactg gtgggtgacc attaacgttg tctggcgcag agaggcggca ttcagcgcct
    13081 ccattacgcg ctgttcactg tgagcatcaa ggctggcagc gggttcatcc aacagtaata
    13141 gcgaacaggg atttagtaac gcacgggcca ccgccacgcg ctgcgcctgc cccacggaaa
    13201 ggcgggcagc ctggtcgcca acaggtgtat caacgccttg tggcaggagc ggtagaaact
    13261 cgctgaccca ggcgttatcc agcgctgctt gtaattcttg ttcgctggca tcaggtcgcg
    13321 ccagtagtac gttatcccgc aatgttgctg ccggtaattg tgggttttgc ccaacccagg
    13381 agagatgttt acgccatgat tctggtgata aatcgcgtaa ttctatcccg ttgattcgta
    13441 atgatccctg atatgagaga aaaccagaaa gcgcgttcag cagtgagctt ttacctgaac
    13501 cgctgcgacc aaccaacact gcacgttggc ctgctggcaa agtaaagttc agcggtccgg
    13561 ccagcgtttt accttccggc gacgtgataa acagatcctc ggcctcaatg gtcaccggat
    13621 cggtcgatgc taattccacc tcgccacgct gcggatgggc gagcggggtt tccataaacg
    13681 ttttcagact gtcagctgca ccaacagcct gggctttagc atgataaaac gtaccgagat
    13741 cgcgcaatgg ctggaaaaac tctggcgcaa ggatcagggc cagaaaaccc gcagccagcg
    13801 tcacaccggt atcgtagtga ccaaaatcca gctcgccgag ataggaaaaa ccaaagtaga
    13861 ccgccaccag agcaattgac agcgaggtaa aaaattcgag aatgccggag gataaaaacg
    13921 ccagccgtag cacttccatt gtccgttggc ggaaatcttc cgaagcagaa cgaatacttt
    13981 caatttcagc ttcaccacga ccaaaaatac gcaatgtttc catgccgcgc aggcgatcga
    14041 ggaaatgccc acttaagcga gcaagagcga gaaagttacg ccggttagca tcggcagccc
    14101 ccatcccaac cagcgccata aacaacggaa ttaacggtgc tgtgcccagc agaatgagcg
    14161 ccgcagccca gttagagggg aagatcgcca ccacaatcag caacggcacc gacactgcca
    14221 gcgccatttg cggcaggtag cgtgcatagt aatcatgcat atcgtcaatt tgctcgagta
    14281 ccaacgtcgc ccagctcccc gcaggtttac cctgaatcca cgctggccct gcttgttgca
    14341 gacggtcgag aacctgacgg cggatggcaa agcggatatg ctgcccggcg tgataaccca
    14401 cccgttcgcg taaccagacc acccatgcgc gcagtacaaa ggtcagaacc agtaacgtaa
    14461 agggaagcag cagggcttca cggggaatat tctccataat catatgttgc agaattcgcg
    14521 ccatgaacca ggcctgggca atgatcaata tgccgcttac aaagcccagc agacgagaaa
    14581 tattcagcca acgttgggag atgacgcttt gctgttttaa ccagcgggtt aactcttttt
    14641 gacgggattt attcattgca cgcttagcag gtgagttatc agaattattt gcagagcaat
    14701 gttacaacgg ggaaaaaata aaggcgaccc atagtcgcat ggtgtcgcct tctttacttt
    14761 tgttactgat ttgtaaaatt attttgcgtc agctaaacca tcgaggtagc gttccgcatc
    14821 aagtgctgcc atgcagcctg taccggccga agtaatggcc tgacgataaa tatgatccat
    14881 cacgtcaccc gcggcaaaga cgccagggat gctggtctgg gtggcattac catgaatacc
    14941 cgactgcact ttgatgtagc cgttttccag ttccagctgc ccttcgaaaa tggcagtatt
    15001 cgggctgtga ccgatagcaa caaacagacc agcaacgtcg agtgactcga tgttatcgct
    15061 gttttgcgta tcgcgcagac gaacgccagt gacacccatt tgatcgccgg tcacttcttc
    15121 cagcgtacgg ttggtgtgca gaatgatgtt gccgttctcc actttatcca tcagacgctt
    15181 aataaggatt ttttccgcgc ggaaaccgtc acggcggtga atcagatgca cttccgaagc
    15241 gatgttagac agatacagcg cctcttcaac cgcggtattg ccgccgccga tgaccgcaac
    15301 tttctggttg cgatagaaga aaccgtcgca ggttgcacaa gcagaaaccc cacggccttt
    15361 aaaggcttct tcagagggca ggccgagata gcgtgcagaa gctccggtgg caataatcag
    15421 cgcgtcgcaa gtgtattcgc cgttatcgcc attcagacgg aacggacggt tttgcagatc
    15481 caccttgttg atatgatcaa aaatgatctc agtttcaaac ttggtggcat gttcgtgcat
    15541 gcgctccatt aataacggac cggtcagatc gtttggatcg ccaggccagt tttccacttc
    15601 cgtggtggtg gtcagttggc cgcctttttc catgccggta atcagcacag gttgcaggtt
    15661 ggcgcgcgcc gcgtagacag cagcggtgta tcccgccggg cctgaaccca ggataagcag
    15721 tttactgtgt ttggtcgtgc ccatgagatc cccatagttg ttggcagaca atgggcagga
    15781 ttgtagggaa tttacagacg taaaaaaaga gtatgacgat tttgttaaca atttgtgcaa
    15841 tcggcagcat cgataagcag gtcaaattct cccgtcatta tcacctctgc tacttaaatt
    15901 tcccgcttta taagccgatt aaatgatgaa taaacgcccc tgttaatgaa tatctggcat
    15961 gttgtactaa aaatcgatgt tttgctttga caatcccctg gtgttttgcg aaaacattcg
    16021 aggaagaaaa aaaacagtat tcttatatgc gcataaccat gcatgtaaat accatgttta
    16081 ccgtgctagt gaaatctacg tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc
    16141 cacaaggcaa cggtcttctc accgtagacc caggcattgc gcgccgtgaa tcttcatgat
    16201 ttcggtctat cgtgacgggt agcgactctg aacagtgatg tttcagggtc agacaggagt
    16261 agggaaggaa tacagagaga caataataat ggtagatagc aagaagcgcc ctggcaaaga
    16321 tctcgaccgt atcgatcgta acattcttaa tgagttgcaa aaggatgggc gtatttctaa
    16381 cgtcgagctt tctaaacgtg tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg
    16441 gctggaaaga caagggttta ttcagggcta tacggcgctg cttaaccccc attatctgga
    16501 tgcatcactt ctggtattcg ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga
    16561 acaattcaat accgctgtac aaaaacttga agaaattcag gagtgtcatt tagtatctgg
    16621 tgatttcgac tacctgttga aaacacgcgt gccggatatg tcagcttacc gtaagttgct
    16681 gggggaaacc ctgctgcgtc tgcctggcgt caatgacaca cggacatacg ttgttatgga
    16741 agaagtcaag cagagtaatc gtctggttat taagacgcgc taacacggaa caggtgcaaa
    16801 atcggcgtat tttgattaca ctcctgttaa tccatacagc aacagtactg gggtaacctg
    16861 gtactgttgt ccgttttagc atcgggcagg aaaagcctgt aacctggaga gcctttcttg
    16921 agccaggaat acactgaaga caaagaagtc acattgacaa agttaagtag cggccgtcgc
    16981 cttctggaag cgttgctgat ccttattgtc ctgtttgccg tctggttgat ggctgcctta
    17041 ctaagcttta acccttcgga ccccagctgg tcgcaaacgg cctggcatga acctatccat
    17101 aatttaggtg ggatgcccgg tgcgtggctg gcagatacgc tgttctttat ttttggcgtg
    17161 atggcttaca ccattcccgt cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag
    17221 tccagtgacg aatacattga ttattttgcc gtttcgctac gcatcattgg cgttttggcg
    17281 ctcatcctta cctcctgtgg tctggcggca atcaacgctg acgatatctg gtattttgcc
    17341 tccggtggcg tcattggcag cttactaagc actacgctac aaccactgct acacagtagc
    17401 gggggaacta ttgcgctgct ctgcgtttgg gctgcgggcc tgacgctgtt caccggttgg
    17461 tcatgggtga ccattgctga aaaactcggc ggctggattt taaacattct caccttcgcc
    17521 agtaatcgta cccgtcgcga tgatacctgg gtcgatgaag atgaatatga agatgacgaa
    17581 gagtatgaag atgaaaacca cggcaaacag catgaatcac gccgtgcccg tattcttcgc
    17641 ggcgcgctag cgcgtcgtaa acggttggcg gaaaaattca ttaatccgat ggggcggcaa
    17701 acagacgctg cgttgttctc cggtaagcgg atggatgatg acgaagagat tatctacact
    17761 gcacgcggtg tggctgccga cccggacgac gtcctatttt cgggcaatcg tgcaacgcag
    17821 ccagaatatg acgaatacga tccattatta aacggtgcgc caattaccga acctgtcgct
    17881 gtagcagctg ctgctaccac ggcgacacaa agctgggctg cgccggttga acctgtgact
    17941 cagacgccgc ctgttgcctc tgttgatgtt ccaccctcgc aacctacagt agcctggcag
    18001 cctgtaccgg gtccacaaac gggagagccg gttattgctc ctgcaccgga aggttaccca
    18061 cagcagtcac aatatgcgca gcctgcagtg caatataatg agccgctgca acaaccagta
    18121 cagccgcagc agccgtatta tgcacctgca gctgaacaac ctgcgcaaca accttactac
    18181 gctcctgcgg cggaacaacc tgttcagcaa ccgtattacg caactgcgcc ggaacagcct
    18241 gcacaacagc cgtattacgc ccctgcgcca gaacaaccgg tggcaggtaa cgcctggcaa
    18301 gccgaagagc agcaatccac ttttgctcca cagtctacat accagactga gcaaacttat
    18361 cagcaaccag ccgctcagga gccgttgtac caacagccgc aatccgttga acggcagcct
    18421 gttgtggagc ctgaacccgt tgtagaagag acaaaacccg cgcgtccgcc gctttactat
    18481 tttgaagaag tggaagagaa gcgagcccgt gaacgtgaac aacttgcggc ctggtatcaa
    18541 ccgattccag aaccggttaa agaaccagaa ccgatcaaat cttcgttgaa agcgccttct
    18601 gttgcagcag tacctccagt agaagccgct gccgctgttt ctccgctggc atctggcgtg
    18661 aaaaaagcga cactggcgac gggggctgcc gcaaccgttg ccgcgccagt cttcagtctg
    18721 gcaaatagcg gtggaccgcg tccgcaagtc aaagagggga ttggcccgca gttgccacga
    18781 ccgaaacgta tccgcgtgcc aactcgtcgt gaactggcgt cttacggtat taagctgccc
    18841 tcacagcgtg cggcggaaga aaaagcccgt gaagcccagc gcaatcagta tgattctggc
    18901 gatcagtaca acgatgatga aatcgatgcg atgcagcagg atgaactggc acgtcagttc
    18961 gcccagacac agcagcaacg ctatggcgaa cagtatcaac atgatgtgcc cgtaaacgca
    19021 gaagatgcag atgctgcggc agaggctgaa ctggctcgtc agtttgcgca aactcaacaa
    19081 caacgttatt ccggcgaaca accggctggg gcgaatccgt tctcgctgga tgattttgaa
    19141 ttttcgccaa tgaaagcgtt gctggatgat ggtccacacg aaccgttgtt tacgccaatt
    19201 gttgaacctg tacagcagcc gcaacaaccg gttgcaccgc agcagcaata tcagcagccg
    19261 caacaaccag ttccgccgca gccgcagtat cagcagccac aacagccggt tgcgccgcag
    19321 ccacaatatc agcagccgca acaaccggtt gcgccacagc cgcagtatca gcagccgcaa
    19381 caaccggttg cgccgcagcc gcagtatcag cagccgcaac aaccggttgc gccgcagcag
    19441 cagtatcagc agccacaaca gccagttgcg ccacaaccgc aggataccct gcttcatccg
    19501 ctgttgatgc gtaatggcga cagccgtccg ttgcataaac cgacgacgcc gctgccttct
    19561 ctggatttgc tgacaccgcc gccgagcgaa gtggagccgg tagatacctt tgcgcttgaa
    19621 caaatggctc gcctggtgga agcgcgtctg gctgatttcc gtattaaagc cgatgttgtc
    19681 aattactctc cggggccggt tatcactcgc tttgaattga acctggcacc gggcgtaaaa
    19741 gcggcgcgca tttctaactt gtcacgggac cttgcccgtt cactttcgac ggtggcggtg
    19801 cgtgtcgttg aagttattcc tggcaaaccc tatgtaggtc tggagttacc gaataaaaaa
    19861 cgacaaaccg tttatctgcg cgaagttttg gataacgcca aattccgcga taatccgtcg
    19921 ccattaaccg tggtgctggg taaagatatc gccggtgagc cggtggttgc cgatctggcg
    19981 aaaatgccgc acttgttggt tgcggggact accggttccg gtaaatctgt cggtgtgaac
    20041 gcgatgatcc tgagcatgct ttataaagca cagccagaag atgtacgttt catcatgatc
    20101 gacccgaaaa tgctggagct ttcggtttat gaaggcattc cgcatctgtt aacggaagtc
    20161 gttactgata tgaaagatgc cgccaacgcg ctgcgctggt gtgttaacga gatggagcgt
    20221 cgttataaac tgatgtctgc gctgggtgtg cgtaatctgg cgggttataa cgaaaaaatt
    20281 gctgaagccg atcgcatgat gcgtccgatt ccagacccgt actggaagcc gggtgacagt
    20341 atggatgccc agcatccggt gctgaaaaaa gaaccataca ttgtggtgtt ggttgacgaa
    20401 tttgccgacc tgatgatgac ggtaggtaaa aaagtggaag agctgatagc acgtctggcg
    20461 caaaaagccc gtgccgcggg tatccacctc gtactggcaa ctcagcgtcc atcggttgat
    20521 gttattactg gtctgattaa agcgaatatt ccgacccgta tcgcctttac cgtatccagt
    20581 aagattgact cacgtaccat tcttgatcag gctggcgcgg aatcactgct ggggatgggg
    20641 gatatgctct actctgggcc gaactccacg ttgccggtac gtgtccatgg tgcttttgtt
    20701 cgcgatcagg aagttcatgc cgtggtgcag gactggaaag cgcgtggtcg cccacagtat
    20761 gttgatggca tcacttccga cagtgaaagc gaaggtggcg cgggcggttt cgatggcgct
    20821 gaagaactgg atccgttgtt cgatcaggcg gtgcagtttg tcactgaaaa acgcaaagcg
    20881 tcaatttctg gcgtacagcg tcagttccgc attggttata accgtgcagc gcgtattatc
    20941 gaacagatgg aagcgcaggg gattgtcagc gaacaggggc acaacggtaa tcgtgaagtg
    21001 ctggccccac cgccgtttga ctaattaatg cattgccgga taaggcgcgg tagcgtcgca
    21061 tccggcactc tatcaactga aaattcagta ttttcttctt tcctcaagct gattattagc
    21121 ctggaataga gagtagaggg aactcccgat cgggagtgac gtaatttgag gaataatgat
    21181 gaaaaaaatt gccatcacct gtgcattact ctcaagctta gtagcaagca gcgtttgggc
    21241 tgatgccgca agcgatctga aaagccgcct ggataaagtc agcagcttcc acgccagctt
    21301 cacacaaaaa gtgactgacg gtagcggcgc ggcggtgcag gaaggtcagg gcgatctgtg
    21361 ggtgaaacgt ccaaacttat tcaactggca tatgacacaa cctgatgaaa gcattctggt
    21421 ttctgacggt aaaacactgt ggttctataa cccgttcgtt gagcaagcta cggcaacctg
    21481 gctgaaagat gccaccggta atacgccgtt tatgctgatt gcccgcaacc agtccagcga
    21541 ctggcagcag tacaatatca aacagaatgg cgatgacttt gtcctgacgc cgaaagccag
    21601 caatggcaat ctgaagcagt tcaccattaa cgtgggacgt gatggcacaa tccatcagtt
    21661 tagcgcggtg gagcaggacg atcagcgcag cagttatcaa ctgaaatccc agcaaaatgg
    21721 ggctgtggat gcagcgaaat ttaccttcac cccgccgcaa ggcgtcacgg tagatgatca
    21781 acgtaagtag aggcacctga gtgagcaatc tgtcgctcga tttttcggat aatacttttc
    21841 aacctctggc cgcgcgtatg cggccagaaa atttagcaca gtatatcggc cagcaacatt
    21901 tgctggctgc ggggaagccg ttgccgcgcg ctatcgaagc cgggcattta cattctatga
    21961 tcctctgggg gccaccgggt accggcaaaa caaccctcgc tgaagtgatt gcccgctatg
    22021 cgaacgctga tgtggaacgt atttctgccg tcacctctgg cgtgaaagag attcgcgagg
    22081 cgatcgagcg cgcccggcaa aaccgcaatg caggtcgccg cactattctt tttgttgacg
    22141 aagtgcaccg attcaacaaa agccagcagg atgcatttct gccacatatt gaagacggca
    22201 ccatcacttt tattggcgca accactgaaa acccgtcgtt tgagcttaat tcggcactgc
    22261 tttcccgtgc ccgtgtctat ctgttgaaat ccctgggtac agaggatatt gagcaagtac
    22321 taactcaggc gatggaagac aagacccgtg gttatggtgg tcaggatatt gttctgccag
    22381 atgaaacacg acgcgccatt gctgaactgg tgaatggcga cgcgcgccgg gcgttaaata
    22441 cgctggaaat gatggcggat atggccgaag tcaatgatag cggtaagcgg gtcctgaagc
    22501 ctgaattatt gaccgaaatc gccggtgagc gaagcgcccg ctttgataac aaaggcgatc
    22561 gcttttacga tctgatttcc gcactgcata agtcggtacg tggtagcgca cccgatgcgg
    22621 cgctgtactg gtatgcgcga attattaccg ccggtggcga tccgttatat gtcgcgcgtc
    22681 gctgtctggc gatagcgtct gaagatgtgg gtaatgctga tccacgagcg atgcaggtgg
    22741 caattgcggc ctgggattgc tttactcgcg ttggcccggc ggaaggtgaa cgcgccattg
    22801 ctcaggcgat tgtttacctg gcctgcgcgc caaaaagcaa cgctgtctac actgcgttta
    22861 aagccgcgct ggcggatgct cgcgaacgtc cggattatga cgtgccggtt catttgcgta
    22921 atgcgccgac gaaattaatg aaggaaatgg gctacgggca ggaatatcgt tacgctcatg
    22981 atgaagcaaa cgcttatgct gccggtgagg tttacttccc gccggaaata gcacaaacac
    23041 gctattattt cccgacaaac aggggccttg aaggcaagat tggcgaaaag ctcgcctggc
    23101 tggctgaaca ggatcaaaat agccccataa aacgctaccg ttaatgttat cgttgcggta
    23161 atgttgttac tgtatccctg tggtcgcagg ctgtggccac atctcccatt taattcgata
    23221 agcacaggat aagcatgctc gatcccaatc tgctgcgtaa tgagccagac gcagtcgctg
    23281 aaaaactggc acgccggggc tttaagctgg atgtagataa gctgggcgct cttgaagagc
    23341 gtcgtaaagt attgcaggtc aaaacggaaa acctgcaagc ggagcgtaac tcccgatcga
    23401 aatccattgg ccaggcgaaa gcgcgcgggg aagatatcga gcctttacgt ctggaagtga
    23461 acaaactggg cgaagagctg gatgcagcaa aagccgagct ggatgcttta caggctgaaa
    23521 ttcgcgatat cgcgctgacc atccctaacc tgcctgcaga tgaagtgccg gtaggtaaag
    23581 acgaaaatga caacgtcgaa gtcagccgct ggggcacccc gcgtgagttt gactttgaag
    23641 ttcgtgacca tgtgacgctg ggtgaaatgc actctggcct cgactttgca gccgcagtta
    23701 agctgaccgg ttcccgcttt gtggtaatga aagggcagat tgctcgcatg caccgcgcac
    23761 tgtcgcagtt tatgctggat ctgcataccg aacagcatgg ctacagtgag aactatgttc
    23821 cgtacctggt taaccaggac acgctgtacg gtacgggtca gctgccgaaa tttgctggcg
    23881 atctgttcca tactcgtccg ctggaagaag aagcagacac cagtaactat gcgctgatcc
    23941 caacggcaga agttccgctg accaacctgg tacgcggtga aatcattgat gaagatgatc
    24001 tgccaattaa gatgaccgcc cacaccccat gcttccgttc tgaagccggt tcatatggtc
    24061 gtgacacccg tggtctgatc cgtatgcacc agttcgacaa agttgaaatg gtgcagatcg
    24121 tgcgcccgga agactcaatg gcggcgctgg aagagatgac tggtcatgca gaaaaagtcc
    24181 tgcaactgct gggcctaccg taccgtaaaa ttatcctttg taccggcgac atgggctttg
    24241 gcgcttgcaa aacttacgac ctggaagtat ggatcccggc acagaacacc taccgcgaga
    24301 tctcttcctg ctccaacgtt tgggatttcc aggcgcgtcg tatgcaggct cgttgccgca
    24361 gcaagtcgga caagaaaacc cgtctggttc ataccctgaa cggttctggt ctggctgttg
    24421 gtcgtacgct ggttgcagta atggaaaact atcagcaggc tgatggtcgt attgaagtac
    24481 cagaagttct gcgtccgtat atgaacggac tggaatatat tggctaatac ccaatttttc
    24541 tgaatctaaa aagcgcctgc gggcgctttt tttgtctccc tttgataccg aacaataatt
    24601 actcctcact tacacgtaat actactttcg agtgaaaatc tacctatctc tttgattttc
    24661 aaattattcg atgtatacaa gcctatatag cgagctgcta tagaaataat tacacaatac
    24721 ggtttgttac tggaatcaat cgtgagcaag cttgagtgag ccattatgaa aacgaaaatc
    24781 cctgatgcgg tattggctgc tgaggtgagt cgccgtggtt tggtaaaaac gacagcgatc
    24841 ggcggcctgg caatggccag cagcgcatta acattacctt ttagtcggat tgcgcacgct
    24901 gtcgatagcg ccattccaac aaaatcagac gaaaaggtta tctggagcgc ctgtacagtt
    24961 aactgtggta gtcgctgccc actacgtatg cacgtcgtgg acggtgaaat caaatatgtc
    25021 gaaacggaca ataccggcga tgacaattac gacggcctgc accaggttcg cgcctgtctg
    25081 cgtggacgtt ccatgcgtcg ccgtgtctat aatccggacc gcctgaaata tccgatgaaa
    25141 cgagtcgggg cgcgcggtga aggcaaattt gagcgcatta gctgggaaga agcctacgac
    25201 atcatcgcga ccaatatgca gcgcctgatc aaagagtacg gcaacgaatc catctatctg
    25261 aactatggca ccggtacgct gggcggcacc atgacccgct cctggccccc gggaaatacc
    25321 ctggtcgcgc gcctgatgaa ctgctgcggc ggttatctga accattacgg tgactactct
    25381 tccgcgcaaa ttgcggaagg tttgaactat acctacggcg gctgggcaga tggtaacagc
    25441 ccgtcggata tcgaaaacag taaactggta gtgctgtttg gtaataaccc tggcgaaacg
    25501 cgaatgagtg gcggtggggt gacttactat cttgaacagg cacgccagaa atctaatgcc
    25561 cgcatgatca tcatcgatcc gcgctatacc gacaccggtg ccgggcgcga agatgagtgg
    25621 atccctattc gtccgggaac agatgccgca ctggttaacg gtctggcgta cgtcatgatc
    25681 actgaaaacc tggtggatca ggcattcctc gataaatatt gcgttggcta cgatgagaaa
    25741 accctgccag ccagtgcgcc gaaaaatggc cactataaag cttatattct gggtgaaggg
    25801 ccagatggcg tggctaaaac accggaatgg gcctcacaaa tcaccggtgt tccggcagac
    25861 aaaatcatca aactggctcg tgaaatcggc agtaccaaac cggcgtttat cagccaggga
    25921 tggggcccgc agcgtcacgc taacggtgaa atcgcaaccc gtgctatctc gatgctggcg
    25981 attctgaccg gtaacgttgg tattaacgga ggcaacagcg gcgcgcgtga aggttcatac
    26041 agcttaccgt ttgtccgtat gccgaccttg gaaaacccga tccagaccag catttcgatg
    26101 tttatgtgga ccgatgccat tgaacgtggc ccggaaatga cggcgctgcg tgatggtgta
    26161 cgcgggaaag ataagctgga tgtgccgatc aaaatgatct ggaactatgc cggtaactgc
    26221 ctgattaacc agcattctga aatcaaccgt acccatgaaa tccttcagga tgataagaag
    26281 tgcgagctga ttgtggttat cgactgccac atgacctcat cggcgaaata tgctgacatc
    26341 ctgctgcctg actgcaccgc ttccgaacag atggactttg cgctggatgc atcctgcggg
    26401 aatatgtctt acgtgatttt caacgatcag gtgattaaac cgcgctttga atgtaagacc
    26461 atctatgaaa tgaccagcga actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa
    26521 ggccgtaccc aggaagagtg gatgcggcat ctgtatgccc agtcgcggga agcgattcct
    26581 gaactgccaa cgtttgaaga gttccgcaag caggggatct ttaaaaagcg cgacccacaa
    26641 gggcatcacg ttgcttataa agccttccgt gaagatccgc aagcaaaccc actgactacg
    26701 ccatcgggca aaattgagat ttattcgcag gcgctggctg acattgccgc tacctgggaa
    26761 ttgccagaag gcgatgtgat cgatccactg ccgatctaca cgccgggctt tgaaagttat
    26821 caggatccgc tgaacaaaca gtatccgctg cagcttacgg gtttccacta taaatctcgc
    26881 gttcactcaa cttacggcaa cgttgatgtg ctgaaagcgg cttgccgtca ggaaatgtgg
    26941 atcaacccgc ttgatgcgca aaaacgcggt atcaacaacg gcgataaagt caggatcttt
    27001 aacgatcgtg gtgaagttca tattgaggcg aaagtgacgc cacgaatgat gccgggtgtg
    27061 gtcgcactgg gtgaaggtgc ctggtatgac ccggatacaa aacgtgtcga taagggcggt
    27121 tgtatcaacg tactgaccac tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat
    27181 acaaaccttg ttcaggttga aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt
    27241 ttttattgat tccagccgtt gcaccggttg caaaacctgc gagctggcct gtaaagacta
    27301 caaagatttg acgccagaag tcagcttccg ccgcatttat gaatatgctg gcggcgactg
    27361 gcaggaagat aacggtgtct ggcaccagaa cgtgtttgcc tactatctgt cgatttcatg
    27421 taaccactgc gaagatccgg cttgtactaa agtctgcccg agcggtgcga tgcataaacg
    27481 tgaagatggt tttgttgtgg tcgatgaaga tgtgtgcatt ggctgccgct actgccatat
    27541 ggcttgcccg tatggcgcac cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga
    27601 tggttgttat gaccgtgttg cggagggtaa aaagccgatc tgtgttgaat cttgtccgct
    27661 gcgggcgctg gatttcggcc ctatcgacga gctgcgtaaa aaacatggcg atctggcggc
    27721 cgttgcgccg ttgccgcgag ctcactttac caaaccgaat attgtgatca aacccaatgc
    27781 caatagccgc ccgaccgggg ataccactgg ctatctggca aacccgaagg aggtgtgaga
    27841 tgggaagtgg atggcatgaa tggccgctga tgatcttcac ggtcttcggg caatgtgtag
    27901 caggtggttt tatcgttctg gctttggcgc tgctcaaagg cgacctgcga gcagaagccc
    27961 agcagcgtgt tatcgcctgc atgtttggtt tatgggtgct gatgggcatt ggctttatcg
    28021 cctctatgct ccatcttggt tcaccaatgc gcgcttttaa ctcgctcaac cgggtagggg
    28081 cttcagcact cagtaacgaa atcgccagcg gttcgatctt ttttgccgta ggcggcatcg
    28141 gctggttgct ggcaatgctg aaaaagctgt caccggcatt gcgtacgcta tggctggtgg
    28201 tgacgatggt tcttggcgtc gtctttgtct ggatgatggt gcgtgtgtat aacagcattg
    28261 ataccgttcc gacctggtac agcatctgga cgccgatggg cttcttcctg acgatgttca
    28321 tgggcggccc gttactgggt tacctgttgc tgagtctggc tggcgtcgat ggctgggcga
    28381 tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggtgtg atgtcagtga
    28441 tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca gcgctggtgc
    28501 cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt
    28561 ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc gtctcattca
    28621 ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg catatgaccg
    28681 tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg gttctctttt
    28741 atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca ggaatatggt
    28801 tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc agctcgcacg
    28861 ccacgccgaa ccatgtcatc aattgcgcac ctgcttgcga catgcgatcc catgccgaat
    28921 gccgggtaat ttcattaaag gtccctgacg cgtcagtcac aacaaagaca tcaaaccctt
    28981 cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca ccggcaataa
    29041 ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta tcccaggcgt
    29101 taatatttcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc agttctggaa
    29161 ctaatggacc gttaggaccg gtttcaaaac tggtggtaag aatagtaggt aagttgaaat
    29221 acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt tcgatatccc
    29281 gtacaaggga aagtaaacca gcctgatgat caacaagcaa aacggcagca tcatttttat
    29341 caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg gtacgtctga
    29401 aatcggtcag acgtgatgca ttgctctgaa agcatagacg ggaaatatga gtttgctgtg
    29461 accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca ataaaaacaa
    29521 tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata taaatcgata
    29581 ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg gcggtggcat
    29641 gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta tacccggcct
    29701 gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt gttaaataca
    29761 ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg cgttgtcagc
    29821 tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt cattaagcgc
    29881 attggcttta accgcagcta ttatctggcc tccttcattt ttgccgctgg ctgtgccggc
    29941 cttggcctga tgattggatt ctggagctgg ttggcttggc gttttgtcgc gggcgtcggc
    30001 tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac
    30061 cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt tttaggccag
    30121 ttactggtca gcaaagtttc aaccgagctg atgtccgtat tgccgtgggt tacaggtttg
    30181 acgttggcag ggatcttacc gctgttgttt acgcgtgtgc tgaatcagca ggctgaaaac
    30241 catgattcga cgtcaattac gtcaatgcta aaactccgtc aggcgcggct tggcgtgaat
    30301 ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc gctgtacctc
    30361 aatcacaaag gggtgagcaa tgccagcatt ggtttctgga tggcggtact ggtcagtgcg
    30421 ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg actgctggtg
    30481 ttgcgtgttc aggtctttgt cgtcattctc ggcagtatcg cgatgcttag ccaggcggcg
    30541 atggcccctg cgttattcat tctcggtgcc gctggcttta cgctatatcc ggtggcgatg
    30601 gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca ggccttactg
    30661 ttgagctata ccgtgggaag tctgcttggc ccgtcattta ccgctatgct aatgcagaat
    30721 ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta tttgctgatg
    30781 ctgctgcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc
    30841 agagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg acttgcttta
    30901 ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt gcatttcgct
    30961 atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc cctttaattt
    31021 tggcgaagga tttgtctatg gctgggaatg ttcaggaaaa acagttgcga tggtacaaca
    31081 ttgcgctgat gtcttttatc actgtctggg gttttggcaa cgttgttaat aactatgcca
    31141 accaggggct ggtggttgtt ttttcatggg tgtttatctt tgcactctat ttcacacctt
    31201 atgcgctaat tgttggtcag ttaggctcga ccttcaaaga tgggaagggc ggggtcagta
    31261 cctggattaa acacacgatg ggacccggac tggcttatct cgccgcgtgg acctactggg
    31321 tggtgcatat tccctatctg gcacaaaaac cccaggcaat tctgattgcg ctcggttggg
    31381 cgatgaaagg cgacggttcg ctaatcaaag aatattcagt cgtagcgtta caggggttaa
    31441 cgctggtgct gtttatcttc tttatgtggg ttgcttcacg cggtatgaaa tcgctgaaaa
    31501 tcgtcggttc tgtggcaggg attgcgatgt ttgttatgtc gctcctgtat gtggcgatgg
    31561 cggtaaccgc gcctgcgatt actgaagtgc atattgcgac cacaaacatt acctgggaaa
    31621 cgttcattcc tcatatcgac tttacctaca ttaccactat ttcaatgctg gttttcgcgg
    31681 ttggcggagc agagaagatt tctccttacg ttaatcaaac gcgcaaccca ggaaaagaat
    31741 ttccaaaagg gatgctatgc ctggcggtga tggttgcggt ttgtgccatt ctgggctcac
    31801 tggcgatggg gatgatgttt gactcgcgta atatcccgga tgacttaatg acgaacggtc
    31861 aatattacgc ctttcagaag ctgggcgagt attacaacat gggtaatact ttaatggtga
    31921 tttacgccat tgcgaatacc ctgggacaag tagctgcgct ggtattctcg attgatgccc
    31981 cgcttaaagt gctattaggt gatgctgaca gcaaatatat tccagccagt ttatgtcgta
    32041 ccaacgcttc tggtacgccc gttaatggct attttctgac cctggtgctg gtggcaattc
    32101 tgattatgct gccgactctc ggcatcggtg atatgaacaa tctctataaa tggctgttga
    32161 atcttaactc ggtagtgatg ccgctgcgtt atttatgggt atttgttgca tttattgcag
    32221 tcgttcgcct ggcgcagaaa tataaaccag aatatgtctt tattcgtaat aagcctctgg
    32281 caatgaccgt cgggatttgg tgttttgcct ttaccgcctt tgcctgtttg acggggatct
    32341 tcccgaaaat ggaagccttc actgcagagt ggaccttcca gttggcgctg aatgttgcaa
    32401 cgccgtttgt gctggtagga ttaggactga tattcccgct gctggcgcgt aaagcgaata
    32461 gtaaataatt attgtggtcg gtgacgctga ctctggcaga tctgacccgt ttttacataa
    32521 tcgagaaatg cgcgcaagcc gcaggacata tactggcggt ttggataata gatctggaag
    32581 cctgaacgct ctgtgctcca gtcttccagt acacattcta aacgaccagt atcaaggtac
    32641 tccttgatct gttcatagag cagatacccg ataccagcac ccatcaaaac ggcctccagt
    32701 tcagcatcaa catcatccag aatgatattt cctgcgacag caatttccag ctcctttgca
    32761 aattgccagt ggaaaggttt gccgctggga taacgaaaaa caacacattg atgattaagc
    32821 agatcatgag gatggcgggg tttgccatat cttgcaaagt actctggcgt tgctgctaca
    32881 cataatttca ctggtgggcc gatcgccacg ctgatcatat ctttttcgac gatgccacta
    32941 agtcgaacgc cagcgtcaaa gccctgttgg acgatatcga caagagaatc gtcagttgtg
    33001 agctcgactt taatatcagg atattcacgt gtgaaaccaa ccagtaaagg cattaaaaat
    33061 atacgtgcag ctacccgggc ggcattgatt ttgagcgttc ctgtcggtgt cagacgaaaa
    33121 tcattcatct catctaacat gatctgaatt tcgtcgaaag ctgggcgcaa tcgttcatag
    33181 agattagaac ccgcttccgt taacgatacg cttcgggttg tccgattgaa taagcgaatt
    33241 ttaagacgtt gttccagtgt tttaatacta tggctaatgg cggacgagga taagcctaat
    33301 tcatcgcccg ctgcacgaaa gctttgatta cgggccacgg caaagaaagt ggcaaagtca
    33361 gacatattca tccgcattag tgaaaatcct tcattaactc atcctgaatc atcacgttta
    33421 caggattatg ccgcctgcgt aaagtgcgct ccagaactta acgtggaggt aaaattatgc
    33481 agtctgaacg tatttatttg gtatgggccc atcctcgtca tgattcattg accgcacata
    33541 ttgctgatgc gatccatcag cgggcaatgg agcggaaaat acaggtgacg gaactcgatt
    33601 tatatcggcg taatttcaac ccggtgatga cgtcggaaga tgaaccagac tggaagaata
    33661 tggataaacg ttattctcca gaggttcatc agctttattc agagctgctt gagcatgaca
    33721 cgttagtggt ggtttttcct ctctggtggt acagcttccc ggcaatgcta aaaggatata
    33781 ttgacagagt atggaataat gggctggctt atggagatgg gcacaaatta ccattcaata
    33841 aagttcgttg ggtggcgctg gttggaggag acaaagaatc atttgtccag atgggctggg
    33901 aaaaaaatat aagcgattat ttaaaaaata tgtgcagtta tcttggtatt gaagatgccg
    33961 atgtcacttt cttgtgtaat acagtggtat tcgatgggga agaacttcac gcgagctatt
    34021 atcagtcgtt attatctcag gtacgggata tggtagatgc actataagat gtgttaaaaa
    34081 cgctgtagca gaatgaagcg cggaataaaa aagcggcaac tcaataaagt tgccgcttta
    34141 cggggaaatt agaacattac cttatgaccg tactgctcaa gaatgccttt cacgcgttcc
    34201 atggtctctt tcttcggtgg tttaacaccg tcgagtttgt actcttcacc cattgccacc
    34261 catttgtgtt tgcccagctc gtggtagggg agaagctcga ttttctcaac gttgcccata
    34321 tcacgggtaa attcaccgag gcgatgcgct gaatcgtcat cgtcagacca gcctgggaca
    34381 acaacgtagc ggatccacac cttcacattt ttgttcgcca gatatttggc gaactccagc
    34441 gtgcggtgat tggaaactcc aaccagattt tggtggatct cgtcgttcat ctgtttgaga
    34501 tcgagcatta ccaggtcggt tacttccagc agttcatcaa tcaccggatc gtaacgacga
    34561 acaaaaccgt tggtgtccag acaggtatga atgccttctt ttttgcaggc gcggaaccag
    34621 tcacgaacaa actcagcttg taggattgcc tcaccgccgg atgcggtaac gccgccgccg
    34681 gaagcgttca taaagtggcg ataggtcacc acctctttca ttaaatcttc aacggtaact
    34741 tctttaccgc catgcgtatc ccaggtgtcg cggttatgac aatacaggca gcgcatcagg
    34801 cagccctgga aaaaggtgat aaagcgaata cctgggccgt ctacggttcc acaggattca
    34861 aaggagtgaa tgcgaccaat aactgacatt gcggtgtttc tccagatgtg gcccatctga
    34921 ggccgtgttg gtgcgcagct cgaaggctac gtcgagtctg ttttggcagt caccttagag
    34981 tatagatagc tgacaaaaaa ggctctcgtg ctaaaaaagg ccccactttc gtggagcctt
    35041 tattgtacgc tttttactgt acgatttcag tcaaatctaa ttacatagat tgagtgaagg
    35101 tacgagtaat aacgtcctgc tgctgttctt tagtcagcga gttgaaacgt actgcgtagc
    35161 cagatacacg gatggtcagc tgcggatatt tttccgggtt ttccatcgcg tcgagcagca
    35221 tttcacggtt catcacgtta acgttcaggt gctgaccacc ttcgatggat gcttcgtggt
    35281 ggaagtaacc atccatcaga ccagccaggt tggtcttacg aacttcgtcg tctttaccca
    35341 gtgcgttcgg aacgatagag aaggtgtagg agataccatc tttagcgtaa gcaaacggca
    35401 gtttagcaac ggaagtcaga gacgctacag cacctttctg gtcacgaccg tgcatcgggt
    35461 tagcacccgg tccgaacggc gcgccagcac gacgaccgtc tggggtgtta ccagttttct
    35521 taccatacac aacgttagaa gtgatggtca gaacagactg agtcgggata gcgtcacggt
    35581 aggtgtgcag tttctgaatt ttcttcatga aacgttctac caggtcaaca gccaggtcat
    35641 ctacacgcgg atcattgtta ccaaactgcg ggtattcgcc ttcgatttcg aagtcgatag
    35701 caagaccgtc ttcgtcacga atcggtttaa ctttcgcata tttgattgca gacagggagt
    35761 cagcagcaac ggacagacca gcgataccac acgccatggt gcggataacg tcacggtcgt
    35821 gcagcgccat cagagaggct tcgtagctgt acttgtcgtg catgtagtgg atgatgttca
    35881 gtgcagtgat gtactgttta gccagccagt ccatgaagtg atccatgcgc tccatcactt
    35941 catcgtagtt caggagatcg cctttgatcg gttcagactt cggaccaacc tgcattttca
    36001 gtttttcgtc aacgccgccg ttgattgcgt acagcatggt tttcgccagg tttgcacgcg
    36061 caccgaagaa ctgcatttgt ttaccaacga tcatcgggct tacgcagcaa gcgatagcat
    36121 agtcatcgtt gttgaagtcc ggacgcatca ggtcatcgtt ctcatactgc agagaagagg
    36181 tgtcgatgga cactttagcg gcgaatttct tgaagttcag cggcagtttt tcagaccaca
    36241 gaatggtcat gttcggttcc ggagacggac ccatggtgta cagggtgttc aggaaacgga
    36301 agctgttttt ggtaaccagg gtacgaccgt cgaggcccat accaccgata gattcggttg
    36361 cccagatcgg gtcgccagag aacagttcat cgtattccgg agtacgcagg aagcgaacca
    36421 tacgcagttt catgaccagg tggtcaacca tttcctgcgc ttcttgttcg gtgatcttgc
    36481 cagctttcag gtcacgttcg atgtacacat ccaggaaggt ggaggtacga ccgaaggaca
    36541 ttgcagcacc gttctgagac ttaacagcag ccaggtagcc gaagtaagtc cactggatag
    36601 cttcctgagc gttggtagcc ggaccagaga tgtcgtagcc gtatttcgca gccatttctt
    36661 tcatctgacc cagagcgcgg tgctgttcag cgatttcttc gcgcagacgg atagtctgtt
    36721 ccaggtttac gccgttttcc agatcagcct gcagagaagt gaactgtgcc agtttgtctt
    36781 tcatcaggta gtcgataccg tacagcgcaa cgcgacggta gtcaccgatg atacggccac
    36841 ggccatatgc atctggcaga ccggtcagaa caccagattt acggcaacgc aggatgtccg
    36901 gagtgtaaac gtcgaacacg ccctggttgt gagttttacg gtattcagtg aagatttttt
    36961 tgatcatcgg atccagttcg cggttgtacg ctttgcagga accttcgatc attttgatac
    37021 caccgaacgg gataagagca cgtttcagcg gagcttcagt ctgcagacca acgattttct
    37081 caagctgctt gttgatgtag ccagcgtcgt gagaggtgat ggtggaagca acagcggtgt
    37141 caaagtcaac tggcgcgtga gtgcggtttt ccagtttaac gccttccatt actttgtccc
    37201 acagggtggt ggtcgcttca gtagcgccag ccaggaagga ctcgtcaccc tcgtacggag
    37261 tgtagttttt ctgaatgaag tcacggacgt ttacttcatt ctgccagtca cctttggtaa
    37321 aaccttccca ggctgtggct aacttttcat taagctcgga catgtaacac ctaccttctt
    37381 aagtggattt tttatttact gcgtacttcg acaaccatta atggtgatcg ttttcacgca
    37441 ggtaaatgac ccagtatgtc aacccaacca acaaaccacc gccgataata ttaccgatcg
    37501 taaccggaat caggttatca gtgatgaagt tcatcacggt caggtgagaa aaattttccg
    37561 gtgcagaacc gacagcggtc cagaattccg gagatgcgaa gtcgcggatt acaataccca
    37621 tcgggatcat aaacatgttt gcgatactgt gctcaaaacc gctggcaaca aacatcgcga
    37681 ccggcagcac cataatgaac gctttgtcca tcaggctacg gccagaataa ctcatccata
    37741 ctgccagaca taccatcagg tttgccagga taccaaggca gacggcctca ataaaagtat
    37801 ggtgcacttt gtggtcggcg gtttgtagga cgtttagtcc ccattgacca tttgcggtca
    37861 tatactcgcc ggaaagccac attaaaagta caaacagcag tgcgccgacc aggttgccaa
    37921 aatagacatt tagccagttt ttcgccaact gaccccaggt gatgcgccca ctcgccttag
    37981 caacaacaat caacactgtg gaagtaaaga gatcggctcc gcagacaaca caaagaatca
    38041 gccccagaga gaagcaaatg ccgccaacca gttttgccat gccgaagggc attgtgcctg
    38101 tgccagtggt tgctgtgata tagaagacga atgcgattga gatgaaaaca ccggcggtaa
    38161 tcgccagata gaaagtctta agcggatgtt tcgttgcttt atagacaccc gcctcttcgg
    38221 ccactttggc cattgcagca ggaagtaaaa gatcaaaagg gttgtcagct ttcacactaa
    38281 ctctctcttt attaagtcgg cgacgagata ctaacaaagc attatagatg agaaattgat
    38341 atagatcata tctcgcctgg cttataggcc cgtaactcgc
//
LOCUS       DAPGWD010000029        38255 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPGWD010000029 DAPGWD010000000
VERSION     DAPGWD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.029"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            155..316
                     /locus_tag="RHX88_001427"
     CDS             155..316
                     /locus_tag="RHX88_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230136.1"
                     /translation="MEFSFSPKLFVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(400..1635)
                     /locus_tag="RHX88_001428"
     CDS             complement(400..1635)
                     /locus_tag="RHX88_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV2230137.1"
                     /translation="MLQQIPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKSVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1782..1858)
                     /locus_tag="RHX88_001429"
     tRNA            complement(1782..1858)
                     /locus_tag="RHX88_001429"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1822..1824),aa:Pro,seq:tgg)
     gene            complement(1901..1987)
                     /locus_tag="RHX88_001430"
     tRNA            complement(1901..1987)
                     /locus_tag="RHX88_001430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1951..1953),aa:Leu,seq:cag)
     gene            complement(2008..2083)
                     /locus_tag="RHX88_001431"
     tRNA            complement(2008..2083)
                     /locus_tag="RHX88_001431"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2048..2050),aa:His,seq:gtg)
     gene            complement(2142..2218)
                     /locus_tag="RHX88_001432"
     tRNA            complement(2142..2218)
                     /locus_tag="RHX88_001432"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2182..2184),aa:Arg,seq:ccg)
     gene            complement(2321..3706)
                     /locus_tag="RHX88_001433"
     CDS             complement(2321..3706)
                     /locus_tag="RHX88_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2230138.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKVHKLEE"
     gene            complement(3897..4637)
                     /gene="rffM"
                     /locus_tag="RHX88_001434"
     CDS             complement(3897..4637)
                     /gene="rffM"
                     /locus_tag="RHX88_001434"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDV2230139.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4640..5992)
                     /gene="wzyE"
                     /locus_tag="RHX88_001435"
     CDS             complement(4640..5992)
                     /gene="wzyE"
                     /locus_tag="RHX88_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDV2230140.1"
                     /translation="MSLLQCSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5989..7068)
                     /gene="rffT"
                     /locus_tag="RHX88_001436"
                     /gene_synonym="wecF"
     CDS             complement(5989..7068)
                     /gene="rffT"
                     /locus_tag="RHX88_001436"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDV2230141.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7065..8315)
                     /gene="wzxE"
                     /locus_tag="RHX88_001437"
     CDS             complement(7065..8315)
                     /gene="wzxE"
                     /locus_tag="RHX88_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDV2230142.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAVASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8317..9447)
                     /gene="rffA"
                     /locus_tag="RHX88_001438"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8317..9447)
                     /gene="rffA"
                     /locus_tag="RHX88_001438"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDV2230143.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9452..10126)
                     /gene="rffC"
                     /locus_tag="RHX88_001439"
                     /gene_synonym="wecD"
     CDS             complement(9452..10126)
                     /gene="rffC"
                     /locus_tag="RHX88_001439"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDV2230144.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10104..10985)
                     /gene="rfbA"
                     /locus_tag="RHX88_001440"
     CDS             complement(10104..10985)
                     /gene="rfbA"
                     /locus_tag="RHX88_001440"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDV2230145.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11004..12071)
                     /gene="rffG"
                     /locus_tag="RHX88_001441"
     CDS             complement(11004..12071)
                     /gene="rffG"
                     /locus_tag="RHX88_001441"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDV2230146.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12071..13333)
                     /gene="wecC"
                     /locus_tag="RHX88_001442"
     CDS             complement(12071..13333)
                     /gene="wecC"
                     /locus_tag="RHX88_001442"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDV2230147.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13330..14460)
                     /gene="wecB"
                     /locus_tag="RHX88_001443"
     CDS             complement(13330..14460)
                     /gene="wecB"
                     /locus_tag="RHX88_001443"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDV2230148.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLIDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14516..15562)
                     /gene="wzzE"
                     /locus_tag="RHX88_001444"
     CDS             complement(14516..15562)
                     /gene="wzzE"
                     /locus_tag="RHX88_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDV2230149.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15574..16677)
                     /gene="wecA"
                     /locus_tag="RHX88_001445"
     CDS             complement(15574..16677)
                     /gene="wecA"
                     /locus_tag="RHX88_001445"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDV2230150.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16917..18176)
                     /gene="rho"
                     /locus_tag="RHX88_001446"
     CDS             complement(16917..18176)
                     /gene="rho"
                     /locus_tag="RHX88_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDV2230151.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18261..18362)
                     /gene="rhoL"
                     /locus_tag="RHX88_001447"
     CDS             complement(18261..18362)
                     /gene="rhoL"
                     /locus_tag="RHX88_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDV2230152.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18503..18832)
                     /gene="trxA"
                     /locus_tag="RHX88_001448"
     CDS             complement(18503..18832)
                     /gene="trxA"
                     /locus_tag="RHX88_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDV2230153.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18963..20228
                     /gene="rhlB"
                     /locus_tag="RHX88_001449"
     CDS             18963..20228
                     /gene="rhlB"
                     /locus_tag="RHX88_001449"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDV2230154.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20362..21846
                     /gene="gppA"
                     /locus_tag="RHX88_001450"
     CDS             20362..21846
                     /gene="gppA"
                     /locus_tag="RHX88_001450"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDV2230155.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21893..23914)
                     /gene="rep"
                     /locus_tag="RHX88_001451"
     CDS             complement(21893..23914)
                     /gene="rep"
                     /locus_tag="RHX88_001451"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDV2230156.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24131..24580
                     /locus_tag="RHX88_001452"
     CDS             24131..24580
                     /locus_tag="RHX88_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDV2230157.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTKYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVNNGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24779..25060
                     /gene="ppiC"
                     /locus_tag="RHX88_001453"
     CDS             24779..25060
                     /gene="ppiC"
                     /locus_tag="RHX88_001453"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDV2230158.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25147..26622)
                     /gene="ilvC"
                     /locus_tag="RHX88_001454"
     CDS             complement(25147..26622)
                     /gene="ilvC"
                     /locus_tag="RHX88_001454"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDV2230159.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQDKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26772..27665
                     /gene="ilvY"
                     /locus_tag="RHX88_001455"
     CDS             26772..27665
                     /gene="ilvY"
                     /locus_tag="RHX88_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDV2230160.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27717..29261)
                     /gene="ilvA"
                     /locus_tag="RHX88_001456"
     CDS             complement(27717..29261)
                     /gene="ilvA"
                     /locus_tag="RHX88_001456"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDV2230161.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29264..31114)
                     /gene="ilvD"
                     /locus_tag="RHX88_001457"
     CDS             complement(29264..31114)
                     /gene="ilvD"
                     /locus_tag="RHX88_001457"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDV2230162.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31179..32108)
                     /gene="ilvE"
                     /locus_tag="RHX88_001458"
     CDS             complement(31179..32108)
                     /gene="ilvE"
                     /locus_tag="RHX88_001458"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDV2230163.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKHIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32128..32391)
                     /gene="ilvM"
                     /locus_tag="RHX88_001459"
     CDS             complement(32128..32391)
                     /gene="ilvM"
                     /locus_tag="RHX88_001459"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDV2230164.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32388..34034)
                     /locus_tag="RHX88_001460"
     CDS             complement(32388..34034)
                     /locus_tag="RHX88_001460"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDV2230165.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34037..34087)
                     /locus_tag="RHX88_001461"
     CDS             complement(34037..34087)
                     /locus_tag="RHX88_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230166.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34174..34272)
                     /gene="ilvL"
                     /locus_tag="RHX88_001462"
     CDS             complement(34174..34272)
                     /gene="ilvL"
                     /locus_tag="RHX88_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDV2230167.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            34625..36145
                     /locus_tag="RHX88_001463"
     CDS             34625..36145
                     /locus_tag="RHX88_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDV2230168.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36170..36508)
                     /gene="maoP"
                     /locus_tag="RHX88_001464"
     CDS             complement(36170..36508)
                     /gene="maoP"
                     /locus_tag="RHX88_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDV2230169.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36627..37466
                     /gene="hdfR"
                     /locus_tag="RHX88_001465"
     CDS             36627..37466
                     /gene="hdfR"
                     /locus_tag="RHX88_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDV2230170.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(37562..37637)
                     /locus_tag="RHX88_001466"
     tRNA            complement(37562..37637)
                     /locus_tag="RHX88_001466"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37602..37604),aa:Trp,seq:cca)
     gene            complement(37646..37722)
                     /locus_tag="RHX88_001467"
     tRNA            complement(37646..37722)
                     /locus_tag="RHX88_001467"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37686..37688),aa:Asp,seq:gtc)
     gene            complement(37777..37892)
                     /gene="rrf"
                     /locus_tag="RHX88_001468"
     rRNA            complement(37777..37892)
                     /gene="rrf"
                     /locus_tag="RHX88_001468"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(37988..>38255)
                     /locus_tag="RHX88_001469"
     rRNA            complement(37988..>38255)
                     /locus_tag="RHX88_001469"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         9328 a        10436 c         9625 g         8866 t
ORIGIN      
        1 acgctctcct gattttccaa ttcattttct ggataaataa ataatttatt tttgtcacta
       61 ttatttatgt aatcatcctg tcagggagag ggatctcaat tatcaatgct taattacgtc
      121 atcattttga tgacatgaaa ccggagcatt ttgcatggaa ttttcgtttt cacccaaact
      181 ttttgttgtt gctgtcgccg ccgctcttcc tctcatggcc agcgcagcag ataccccgtc
      241 aactgccacc gcacgcaaag gctttgccgg atacgatcac ccaaaccagt atccacagaa
      301 cgctgcaatt tattgaattt gcacgatcat gtaggccgga taaggcgttt acgccgcatc
      361 cggcaatcaa ccgcaggcgg ccacaaaatg gccgcttttt tacttattca ccaccagcaa
      421 atgtgcctgc ataatgtcac tggccgggcg accgtgcgcc agcaaatcag ccattgcttt
      481 aagatatggc ggtagatggc ggaaataacg ctgatacccg gcacacaaat aattcagccc
      541 cggtttgccg ctggcatcga gcatgaagcg gtgtttcggg cagcctcccc agcacgcttt
      601 taacacgtta caactgcgac actgcgccgg taattgctta aatttatctt caccaaacac
      661 ctgctgttgc ggggaatcga tcatttctgc aattgtttgc tgatgcatat tccccagccg
      721 atattgcgga taaacatagt gatcgcaggc gtaaacgtcg ccgttgtgct caacaatcac
      781 cgagcgccca caggttggct gatgatggca aaccgcaccc ggcgcaccga caaaattggc
      841 aaacgcccat tcgatattca tcacgaaaat cttgccgacg tcgcgtttga tccagtggtc
      901 gaatatcgcc accagaaatt caccaaattc atcggggcgc accgaccatt ccgtcagttc
      961 cccctgaata tcaccaggcg catgtagttt cagtccatcg ctggcagctg tttcatcagc
     1021 caggcgctcg accaccggaa taaactggat gaattcgact cccgcatcgc acaaaaaatc
     1081 ataaacctgc aacggttgct gcgcgctggt gcggttgacg cagaccagca cgttatagtc
     1141 gacatgatgt ttttgcagca gcgtcagggc acgcatcacc agcttatgcg ttggtctgcc
     1201 acctttggtc acgcgatatt gattgtggat ctcagccgga ccgtccagcg ataacccaac
     1261 aagaaaatga ttttctgcca gaaatgcaca ccatttatca tcgagcagca cgccgttagt
     1321 ctggaagctg ttacttatct tcctgccagc accgtatttc gcctgtaact ttacggcacg
     1381 gcggtaaaaa tccagcccga gtaacgttgg ttcgccgccc tgccaggtaa aagccacttc
     1441 gttttgcggt tcgctggcag cgatatagtg acggacatac gcttccagcg tgtcatcgtc
     1501 catatgcgtg actgactttt cgcggtaaag ggattgtttt tcgagataaa aacagtagtc
     1561 acagttcaga ttacaatcgg aaccgctcgg tttcgccatc acatgaaaag cacgcgttgg
     1621 aatctgttgc agcatggtcg ctccttgcct gaagtcatcc ttcaatacgt taagggcgaa
     1681 atcataccgt ggggcaatga gttattacgg gcgtttattg cgaagggaga gtgacgaaat
     1741 gcacagaaaa caaaaaaccc cgccgaagcg gggttcaaaa ttggtcggcg agagaggatt
     1801 cgaacctccg acccactggt cccaaaccag ttgcgctacc aagctgcgct actcgccgaa
     1861 atactgcttt ttgaattttt agttcaattc tttaaagtcg tggtgcgagg ggggggactt
     1921 gaacccccac gtccgtaagg acactaacac ctgaagctag cgcgtctacc aattccgcca
     1981 ccttcgcatt gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa
     2041 ctggaatcac aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta
     2101 cgcggtatta ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt
     2161 cgaacctgag acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt
     2221 agcgccgttg cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa
     2281 ataaattgcg cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgca
     2341 ctttaccgtg gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga
     2401 tgatgccgac aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc
     2461 aaatcaggaa tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat
     2521 ggctggcaat cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa
     2581 accatggcac catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg
     2641 gattgggaat gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca
     2701 cacccgcaac tggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt
     2761 ttttcgccag tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga
     2821 gcgcagccgt cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg
     2881 caaaagtcag tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga
     2941 cgataacgaa aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg
     3001 cactgcgcag cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca
     3061 cgccctggta ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg
     3121 caaagaaacc gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa
     3181 agccaaagaa aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga
     3241 tcatcgcgaa ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg
     3301 ccaccagcgc cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact
     3361 ggacataaac gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt
     3421 aagaccaggc agtgagatag ccaaagaacg ggctcatata acgatgcgca taaacggcga
     3481 acgaaccggt aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga
     3541 cgaacagccc ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac
     3601 tggcggcccc cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat
     3661 gtcgagcttc cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg
     3721 tgtttgcatg ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg
     3781 ttttcgtaat tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat
     3841 aaaaaagcag gcaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag
     3901 aggttgccgg tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg
     3961 ctcggctgcg aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt
     4021 gcgcgtttta cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca
     4081 tctggatgca ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca
     4141 acggtgacga tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg
     4201 ggtttaaaat aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta
     4261 gcttcagttt gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct
     4321 ttgcctgcgc gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc
     4381 gcctgcgggt actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat
     4441 tcggcagcgt taattaactc cctgacctcg gcgttatctt caatagtcag cattttttca
     4501 gcattaatgg caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg
     4561 gcgtgctgca tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc
     4621 gtggtgttgt tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt
     4681 tatgaatgag tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg
     4741 cgccgaagac cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg
     4801 ccagcacgat catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat
     4861 agcgattagt ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc
     4921 caaccacaat cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta
     4981 taagcgtagg cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt
     5041 tggctgagtt cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat
     5101 agaaatcgcg gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca
     5161 gcaacgccag attctcccat ggcgagaagg tgtcgcgagt gagatagaga aacgtataga
     5221 acgcttcatc gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccca
     5281 caatccccag cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc
     5341 caataaacag gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca
     5401 tataagtcag caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctat
     5461 cctggcgcag aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta
     5521 acgccacgcc ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca
     5581 aaaagccgtt gtgcatgaag aagatgccga cgcttaccag cgcgatgccc atcaggatca
     5641 cccacgtaag attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag
     5701 caacgcgttt gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg
     5761 cagaaagcaa cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata
     5821 ccagcacgct ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga
     5881 agacattgaa gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca
     5941 gcgtgcagag cagccagaca acaaacaggc cactgcattg cagcagactc atgcgacctc
     6001 cccggcggca atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc
     6061 aatggtgttt ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt
     6121 gagatcgtcg gtagtaaaca gcaccggcaa atgctgttcc gtcatatcct gccagaacgg
     6181 attttcccgg ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat
     6241 gccctgctgg cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata
     6301 ggcgtcaaat tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag
     6361 ccccgcctga cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac
     6421 caccactttt accgtatcgc caaattgctg atgaacggcg cacaaggcag caatatggtc
     6481 attgctgcgg tcaccggagt tccccaccag aatggtcatt ttcccttcac gttgccgatc
     6541 gttcgccatc gtattgagag aagggtccat ccgcgtcggg aagtacagca gttcgccccg
     6601 cacctttgga tgcgttttgg caaaaaagct caaatcaccg cgggtggcaa atacacagcc
     6661 gactcgcttt tgcgccaggc gacgtagcgg gtaaaaaagc ttatatctca agccactgga
     6721 aagctcatac aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc
     6781 actcagcaga gccagccaca gtttgggatt gaactgaccg tggaagaaaa aacgctgctg
     6841 acggttagct tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa
     6901 ttgcacagaa agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa
     6961 ctcgcgcgcg tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg
     7021 gttatggtga gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc
     7081 cagagtaaaa acacgccaca acaaagagaa aaatagacga tataagttgc catataggcc
     7141 tgcgccgcgc ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa
     7201 gtgaactggc tgacctccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga
     7261 taaccaaaga cataagcgcc cacttttaac acatcaccca ccaactgcca ggcaaagaga
     7321 tcgcgcatag cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcagc
     7381 cagacggtaa aactcgccac cgccactgcc ggtaagacga atttcagcga tttaaccact
     7441 tcccgtgtga tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg
     7501 aacgatgccg taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc
     7561 cccacttcat cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca
     7621 ggcaaggtca ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga
     7681 ccgttatccc agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg
     7741 atggcggcag gaatcaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca
     7801 taaccgccca aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc
     7861 agcgataacg cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg
     7921 ccccaggcaa tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta
     7981 ttaccaaaca atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc
     8041 gtagagaagc caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga
     8101 ttatcatggt actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc
     8161 acgccgagca cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgcaggacca
     8221 aatgacaccg ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg
     8281 gccgccgtcc acaaggacgc ttttgccaac gacatatcag gaaaagtagt tcaacaaagt
     8341 cgcaattacc gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag
     8401 gcgctcgctc tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc
     8461 cgcagggcaa ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag
     8521 aaagttaatc aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc
     8581 gttctgcacg cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg
     8641 cgctaacgca tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc
     8701 tgcttccagt tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc
     8761 aatatcgcgc caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc
     8821 cttttcacgg atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc
     8881 gccttcaccg cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc
     8941 aatggttccc agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac
     9001 cacaaacaga ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacgcc
     9061 cgcgtaatgc acaggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt
     9121 ttcgtcgata ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag
     9181 cacaaaggca ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg
     9241 ctggatatcg agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa
     9301 cactttggcg ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc
     9361 atcgccacac agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac
     9421 caccggcggt gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt
     9481 ctacattcgc accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg
     9541 ccacccgcaa agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct
     9601 cagcacctgc accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc
     9661 gtaaagagac atagccgcga atatcgccgg acgccgcacg taaaatcaga cattgatgat
     9721 caaaggtgcc gcgcacggca ttttcaatcc actgtgcata aaagcgactg ctggcgtcag
     9781 gcgcatacca cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta
     9841 atgcgggaat atcggtctct tgtgccacta cagcaccgct atcactggca ttgttcacgg
     9901 gtagcgccaa atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca
     9961 gttcacccgt atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacgcgt
    10021 ctggcgtcag gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga
    10081 aggcgttttc ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc
    10141 agatattggc cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca
    10201 tcgagccagc cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt
    10261 ttttctactg tctgcacaaa ggtactggct tcaatcaggc tgtcgtgagt gccagtgtcc
    10321 agccaggcaa atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc
    10381 tggttgatgg aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac
    10441 tccacgactt tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt
    10501 tttggctttt cttccagcga gatagcgcgg aaattgtcgt caaactccac cacgccaaag
    10561 cgttccgggt ccatcacctg atagccaaat accgtcgccc cttccgtgcg cgccgcaaca
    10621 tgacgcagct tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa
    10681 gaaggttcac cgttgaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg
    10741 ctgggctgtt cggcgtattc cagctggata ccgaactcac taccatcgcc cagcaggcgc
    10801 tggaaataac ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccaac
    10861 atcagcaccg acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc
    10921 gatacgccgc gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct
    10981 ttcatgtttg cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg
    11041 ccgtcctgca cctgcttcca ccagctttca ttagccagat accactgcac cgttttacgc
    11101 attccacttt caaaggtttc ctgtggcagc caaccaagtt cacgggcaat tttcgaagca
    11161 tcaatggcat aacgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga
    11221 tagtgcgcca cgccgtgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt
    11281 tccacaacat cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact
    11341 ttcccggtgg tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca
    11401 cggatttgct gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc
    11461 atcagcggga tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg
    11521 atcagcgtcg gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctactggct
    11581 tttgacgcgg aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc
    11641 gtcgagtgca ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat
    11701 tttttatctt ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc
    11761 ccgacaatgt tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt
    11821 tcggctgcca gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct
    11881 gcccgatcgc agatatcaac tttctcaaag gcaaagtgct cgctttgcgc gaccggtgcc
    11941 agcgacatca ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt
    12001 tcgttgatga tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga
    12061 atttttctca tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt
    12121 cgccattgat aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg
    12181 ccagcgcctc gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt
    12241 ggatgttagg ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga
    12301 tttccatcgc cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc
    12361 aggcgatttt cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg
    12421 ctttcacctg atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga
    12481 taagccgcgc ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt
    12541 gaccgcccac gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc
    12601 gaatcagttc ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa
    12661 aagcgatatt cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg
    12721 agttagtgac gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggtcg
    12781 aacaaaccgg cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct
    12841 ggcccggtaa cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct
    12901 gctgtgggaa agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg
    12961 accccaccgg cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa
    13021 tggagcgagc agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg
    13081 taggtacagc aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac
    13141 cgccttctac ggcagttttt actacgctcg ccaaatcagg ttcgacgata tggatttcgc
    13201 cacgattgat ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc
    13261 gtgaggcaaa cgcagctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg
    13321 tcgcaaaact catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc
    13381 ctgaccatca ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtctat
    13441 taaaagacgc gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt
    13501 acccgccgtc accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc
    13561 cagcgaaggc gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt
    13621 catcagccag acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg
    13681 ccccagaatg cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat
    13741 ctggatgtcc tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc
    13801 acgaccgaaa ctttcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat
    13861 aaacgggtaa tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctgatcacg
    13921 cacccataac agtgcatcaa tgactgtatt accagtaatg aagattcggc tatccgcaac
    13981 gttttcacgc agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag
    14041 atgcccggtc aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg
    14101 taaaccagcc tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc
    14161 cagcgtcgtt gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat
    14221 aggttttagc ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat
    14281 tatgttgaga tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc
    14341 ccgatgctgc gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg
    14401 caccaacgga gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac
    14461 atcgattctc ttcgaataag cggcgagcgc ctttgcgctc gcagcagcag tgttgctatt
    14521 tcgagcaacg gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca
    14581 taatcatcag gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca
    14641 aatagcgata ggtctgaaaa cgcggatcca gagttggacc aacattcagg gtgtttaaca
    14701 tggcccgatt ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca
    14761 gtcgagcctg gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg
    14821 caggcacatc tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct
    14881 caatgctgtt catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct
    14941 gagctttcat ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc
    15001 tggctgcacg ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg
    15061 cggtttcggc aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa
    15121 actggatgtt gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca
    15181 tccgctgttt gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca
    15241 gttgcataac aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca
    15301 tgtttgaacg gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca
    15361 tattcaccgt tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag
    15421 tatacgccag cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag
    15481 cccacaaggt acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt
    15541 tcccaggcat tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc
    15601 acgatttcta cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg
    15661 cttaatgcaa tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc
    15721 cgggacaaaa tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag
    15781 ggtaatcagc acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat
    15841 atgctgacgg tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc
    15901 caccatatcc attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg
    15961 ggttttgccc tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact
    16021 gcccgcatca cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat
    16081 gtatggcagg atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc
    16141 gaaccacaaa atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc
    16201 atcaatgcca tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag
    16261 gaagtaacca aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact
    16321 gagataaagt ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg
    16381 gatttttacg ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc
    16441 acaagcgaga tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca
    16501 aatccctgcg taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg
    16561 gaagtttggt ttatccacta aaccgacttt ttttgccact ttacgggcaa aaaacagaaa
    16621 cagtgtcgtg aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat
    16681 gcatgctctc agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg
    16741 gaaagtccgt tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg
    16801 cataacatgc cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta
    16861 cagatataaa agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat
    16921 gagcgtttca tcatttcgaa gaaatcgtca ttggtcttag tcatggccag tttattaatg
    16981 aggaattcca ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt
    17041 ttctgcagtt cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag
    17101 tcgatagccg ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg
    17161 cctgtacctt taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc
    17221 gccgtcgcga taatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag
    17281 cgtttcggac gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc
    17341 ggaacaacag tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg
    17401 tctttcttgt gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg
    17461 tggcgagatg cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc
    17521 atctcggtta cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg
    17581 tggttgtaag caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc
    17641 ggtgccacaa tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga
    17701 gcggttaaat cttccgtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc
    17761 agcggggtta agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta
    17821 acttcgttaa ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta
    17881 ccagagatgg tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag
    17941 atgtcatcag gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc
    18001 tgcaatatct ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc
    18061 aggatggcaa aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt
    18121 tcgccgagag tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg
    18181 gtgtgagttc ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga
    18241 tcatccatgc ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag
    18301 tacgcagaac tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc
    18361 atttaagata aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt
    18421 aatacgaagt caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg
    18481 ggcacccgaa catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac
    18541 ctttagacag tgcacccact ttggttgccg ccacttcacc gtttttgaac agcagcagag
    18601 tcgggatacc acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt
    18661 ttgcaacggt cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca
    18721 ttttgcacgg accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga
    18781 gtacatccgt gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact
    18841 ccacaggaat aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg
    18901 ttgactttat ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca
    18961 ctatgagcaa aacacattta acagaacaga agttttccga cttcgccctg catccgaagg
    19021 ttgtagaagc ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc
    19081 ttccgctgac gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa
    19141 cgatggcgtt tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc
    19201 gcaaggtgaa tcaaccacgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga
    19261 tccatgccga cgcagaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg
    19321 gtggtgatgg ctacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg
    19381 gcactacggg gcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc
    19441 aggtggtggt actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc
    19501 gctggctgtt ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca
    19561 cgctttcgta ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg
    19621 aagtggaacc ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta
    19681 acgaagaaaa aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga
    19741 ttattttcgc caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg
    19801 gtcatcgtgt cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg
    19861 atgaatttac ccgtggcgat ctggatattc tggttgccac tgacgttgcc gcgcgtggtt
    19921 tgcatattcc ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt
    19981 acgttcaccg tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg
    20041 cgtgtgaaga gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc
    20101 cggtaagcaa atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca
    20161 cgcgcccacg tacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt
    20221 caggttaaat aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat
    20281 gaatcccgat gagcatactg tagtaagtga ttcaggtgaa agaatgcagc caacacagag
    20341 acagattgaa ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc
    20401 ggttcgaata gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg
    20461 acgcgaataa aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgccctgtct
    20521 aatgaagcaa tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat
    20581 atccctccct cgcaaattcg cgttgtcgct acggcgacat tacgtcttgc cgtcaatgcg
    20641 ggtgatttta ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt
    20701 gaagaggaag cacgtctgat ttatcagggc gttgctcaca caactggcgg tgccgatcag
    20761 cgcctggtgg tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa
    20821 accacctcgt tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc
    20881 gatcgtaatc tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta
    20941 cgtccggttg ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc
    21001 accgtgcagg cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg
    21061 gaaaagttgc agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag
    21121 attgacgggc tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc
    21181 gccattttta ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa
    21241 ggcctggtct acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacgctg
    21301 cgtaatattc agcgccgctt tatgatcgat attgatcagg cgcagcgcgt agccaaagtt
    21361 gcggctaact tcttcgatca ggtggaaaag gaatggcatc ttgaagcaat aagccgcgat
    21421 ttgctcatca gcgcctgtca gcttcatgaa atcggcctga gcgttgactt taaacaagcg
    21481 ccgcaacacg ctgcttatct ggtgcgtaat ctggatcttc ccggttttac ccccgcacag
    21541 aaaaaattgc tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg
    21601 catcagcaaa atgccgtacc gccgcgcgtc gcagaacaac tctgccgttt actccgcctg
    21661 gccatcattt ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct
    21721 aaccatgaac tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt
    21781 aaagagatta ttgctcagga aagccagtgg cagagctatg tccactggcc gctggaagtg
    21841 cattaatgag taagtgccgg atgcgatgct gacgcatctt ttccggcctt gattatttcc
    21901 ctcgttttgc cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc
    21961 gttcttcggc gctgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct
    22021 ccagcaaaaa gcggctcggc tccgggcgca ccagctcgcc gtactgacgg cgttctttac
    22081 acagcgtaaa ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct
    22141 cctcatcgat attatcttca tcgatgctgc tctggtgcgg caaaaaccct tcttccatac
    22201 cgaccatgta gacataagga aactccagcc cttttgacgc gtgcagagtc atcagttgca
    22261 cctgatccag ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg
    22321 tcaccacctg ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca
    22381 tccagctaaa cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg
    22441 atgtttcgta cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa
    22501 tcggctcccg ctccgccaga cgctggattt ctgccaacca gtgagtaaag cgggtcaatg
    22561 cttcataacc acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca
    22621 tgcttttatt gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct
    22681 ctctcttcgg cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca
    22741 cgcgcagata agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag
    22801 atattttgta tgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatggt
    22861 taccgcgata aagaatggcg taatctttgt actgcgtttt attgacgaag tgatgggcga
    22921 tcagctcgcc agtaacgcgc tcggcttcat gttcctcgtt attcgcgctt aaaactttta
    22981 gctctgtgcc gtaacccagt tcggagaaca gacgcttttc aaagacgtgc ggattattgg
    23041 cgatcaggat gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct
    23101 taatcacctt cagcgccgga aaatcctgac tcagcagcac caagttttgt ggacgtgcgc
    23161 cgcgccagga gtagatcgac tggtcgtcat cgcccaccac ggtaaagcgc gcgcggctgc
    23221 ccaccagcag tttcaccagt tcatactggc tggtgttggt gtcctgatac tcatccacca
    23281 gcagatagcg aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagcagca
    23341 acgtcggcaa taaaatcaga tcatcgaagt cgagaacatt gcacgctttc aggtgtgcat
    23401 catacagccc ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg
    23461 cctgggacgg tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca
    23521 ggagaacttt gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat
    23581 cggtatcgtc aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga
    23641 tgatatctag ccccagcgtg tggaaggttg agatcatcag cccacgcgcc tctttgcgac
    23701 ccagcgtctg tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg
    23761 ccgcgatatg tcgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga
    23821 tcacacgagt tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt
    23881 cgacagcttg ttgttggccg gggtttagac gcataggtgt attgctcaat cttcgaacgg
    23941 ggggaggatt gtagcagaat cgcatttgaa tgtgaccgcg tgtaagccgc taaccggttg
    24001 gctgcggatg tttaaatcag agagagaaga ccgaaaccgg agttcatcgt gaatccatta
    24061 gacttagaaa atatcgggtc gtaacgttgt gttattactt tttcagagtt tactaaggag
    24121 aggtgtactc gtggcgatta atttcagtcc taaggttggt gaaatactgg aatgcaactt
    24181 tggtaactat cctgttagcc agaatggaca gttcagcacc aagtattacg atggccgcat
    24241 tcctcctgaa atgataaaaa acaggttagt tgtcgtgctc aacggaaaaa ttaatggcaa
    24301 cgcatgtatc gtcgttccat tatcaaccac tcgcgatcat gacaagttaa atcgtggaat
    24361 gcatgttgaa atagccagta atgttaacaa tggcctgcaa ttctttgatc agcagattcg
    24421 ttgggccaaa gccgacctgg tacaacaagt cagtcgaaac cgattaaata gagcaaggac
    24481 ttacagaggg tatcttaatc aatgtttgcc acatgagttg gttgcagaca tacagagggc
    24541 ggtcatcaag tcaatcaatg ccatatcgct cataaattga caataaaata accatccccg
    24601 ataatgactt tgtagcccat agaggcccta agttttctcc ccttgaggga cctgcgaagc
    24661 ccggattttc cgggcttttg ccatatatgt accagcccct ccctgactga caattacgtt
    24721 agcatctgcg gatcatgcta aaatcgccgc ctgacaatta ttcactcaag gcacgatcat
    24781 ggcaaaaaca gcagcagcac tgcatatcct tgtaaaagaa gagaaactgg ctctggatct
    24841 tctcgagcag attaagaacg gtgccgattt cggcaagctg gcgaagaaac actctatttg
    24901 cccgtcaggc aaacgcggcg gtgatttagg tgaattccgc cagggtcaga tggttccggc
    24961 gttcgataaa gtggttttct cttgtccggt actggagccg accggcccgc tgcacaccca
    25021 gttcggatat cacatcatta aggtgctgta ccgcaactaa tagcaaggcc ttctccagga
    25081 gaaggccttg agtgttttct ccctctccct gtgggagagg gtcggggtga gggcatcagc
    25141 gcgcacttaa cccgcaacag caatacgttt catatctgtc atatagccgc gcagtttctt
    25201 acctacctgc tcaatcgcat ggctgcgaat cgcttcgttc acatcacgca gttgcgcgtt
    25261 atctaccgcg ccttccggaa tagctttacc caagtcgccc ggttgcagct ctgccataaa
    25321 cggtttcagc aacggcacac aagcgtaaga gaacagatag ttaccgtact cagcggtatc
    25381 agagataacc acgttcattt cgtacagacg cttacgggcg atggtgttgg caatcagcgg
    25441 cagctcgtgc agtgattcat aatatgcaga ctcttcaatg atgccggaat cgaccatggt
    25501 ttcgaacgcc agttcaacgc ccgctttcac catcgcgatc atcagtacgc ctttatcgaa
    25561 gtactcctgc tcgccgattt tgccttcata ctgcggcgcg gtttcaaacg cggttttgcc
    25621 ggtctcttca cgccaggtca gcagtttctt atcatcgttg gcccagtccg ccatcatacc
    25681 ggaagagaat tcgccggaga tgatgtcgtc catatgtttc tggaacagcg gtgccatgat
    25741 ctctttcagc tgttcagaaa gcgcataagc acgcagtttc gccgggttag agagacggtc
    25801 catcatcagg gtgatgccgc cctgcttcag cgcttcggtg atggtttccc aaccgaactg
    25861 aattagtttt tctgcgtatg ccggatcggt gccctcttcc accagcttgt cgaagcacag
    25921 cagcgagcca gcctgcaaca taccgcacag gatggtttgc tcacccatca ggtcagattt
    25981 cacttccgca acgaaggacg attccagcac gcccgcacgg tgaccaccgg ttgctgccgc
    26041 ccatgcttta gcgatcgcca taccttcgcc tttcggatcg ttttccgggt gaacggcaat
    26101 cagcgtcggt acgccgaatc cacgtttgta ctcttcacgc acttcggtgc cagggcattt
    26161 cggcgcaacc atgactacgg tgatatcttt acggatctgc tcgcccactt cgacgatgtt
    26221 gaaaccgtgc gagtaaccca gcgccgcgcc gtctttcatc agtggctgta cggtgcgcac
    26281 tacatcagag tgctgcttgt ccggcgtcag gttaaccacc agatccgcct gcgggatcag
    26341 ttcttcgtaa gtaccaactt taaaaccgtt ttcggtcgct ttacgccagg acgcgcgctt
    26401 ctcggcaatc gcttctttac gcagagcgta ggagatatcg agaccagaat cacgcatgtt
    26461 caggccctgg ttcagcccct gtgcgccaca gccgacgata accacttttt tatcctgaag
    26521 gtagctcgcg ccgtcggcga attcgtcgcg ccccataaag cgacatttgc ccagctgtgc
    26581 cagctgctgg cgcaggttca gtgtattgaa gtagttagcc atggtgattc ctcgtgatgt
    26641 tgtgcttctt attgttcggt tcgcgtttgc gagattgaat tcactatatg acaggaaatt
    26701 tattgcggaa attgatatat tcacaacgtc acattgcaat ttttgcaacg tcaacatcga
    26761 gggctgcgcc attggattta cgcgatctga aaaccttcct gcatctggcg gaaagccgcc
    26821 attttggccg cagcgcgcgg gcgatgcacg ttagcccatc cacgctctca cggcagattc
    26881 agcgcctgga agaagatctt ggtcagccgc tatttgtgcg cgataaccgc acggtaacgc
    26941 tgacggaagc gggcgaagag ctgcgcgttt tcgcccagca aacgctgttg cagtatcagc
    27001 aattgcgcca caccatcgat cagcaagggc cgtcgctctc tggcgaatta catatcttct
    27061 gctcggtgac cgctgcctac agccatctgc cgccgattct ggatcgcttc cgcgcggaac
    27121 acccgtcggt ggagatcaaa ctcaccactg gtgatgcggc agatgcaatg gaaaaagtgg
    27181 tcacgggaga agcggatctg gcgattgccg ggaaaccgga gacgctaccc ggcgctgtgg
    27241 cgttttcgat gctggagaat ctggcagtag tgctgattgc gcccgcgctg ccctgcccgg
    27301 tgcgtaacca ggtttcggca gagaagccgg actggtctac agtgccgttt atcatggccg
    27361 atcagggacc ggtgcgtcgc cgcattgaac tgtggtttcg acgcaataaa atcagcaacc
    27421 cgatgattta cgccacggtt ggcgggcatg aagcgatggt atcgatggtg gcgctcggct
    27481 gtggtgtggc gctgttgccg gaagtagtgc tggaaaacag ccccgaaccg gtgcgtaacc
    27541 gcgtgatgat tttagagcgc agcgatgaaa aaacgccgtt tgaactgggc gtgtgtgcac
    27601 aaaaaaagcg gctgcatgag ccgctgattg aggcgttctg gaggattttg ccgaaccaca
    27661 aatgacgttg tcgcgcgggt aggcctgata agcgaagcgc tatcaggcat ttttccctaa
    27721 cccgccaaaa agaacctgaa cgccgggtta ttggtttcgt cgtggcaatc gtagcccagc
    27781 tcattcagcc gggtttcgaa atccggttca tggtcgccaa gttcgaacgc cgccagtacg
    27841 cgcccgtagt cggtgccatg gctgcgatag tggaacaaag aaatgttcca gtgcgtaccc
    27901 agcgtgttga ggaagcgcag cagcgcgccc ggtgattccg ggaattcgaa gctgtagagg
    27961 cgttcctgca acggatgtga cggacgcccg ccgaccatat agcgcacatg caacttcgcc
    28021 atttcgtcgt cggagagatc caccacgctg tagccgccgt cgttgagcat ctgcaaaatt
    28081 tctttgcgct cttcgaggcc gcggctcagg cgcacgccga caaagatgca ggcgtttttg
    28141 gcatcggcaa aacggtagtt gaactcggtg accgatcgcc cgccaagcag ttggcagaat
    28201 ttgaggaagc tgcctttttc ttccggaatg gtcaccgcca acaacgcttc acgctgttcg
    28261 cccagttcgc agcgttccga gacgtagcgc aggccgtgga agttcacgtt ggcaccggaa
    28321 agaatatgcg ccagccgttc gccgcgaatg ttgtgctggg cgatatattt tttcattccc
    28381 gccagcgcca gcgcgccaga gggttccgcc accgcgcgca catcttcgaa taaatccttc
    28441 atcgccgcac agatcgcatc actatcgacg gtgatgatgt cgtcgagata ctcctggcat
    28501 aaacggaagg tttcgtcacc gatgcgtttt accgctacgc cttcagcaaa tagccctacg
    28561 cgcggcagat caaccggatg acccgcatcc agcgctgctt tcaggcaggc ggagtcttcc
    28621 gcttctacgg cgatcacttt gatttgtggc atcagttgtt tgatcagcac cgcaacgccc
    28681 gccgccagac cgccgccgcc gactggcaca aatacgcggt cgagatgggc gtcctgctgg
    28741 agcagttcca gcgccagcgt gccttgcccg gcaatcacca tcggatggtc gaacggcggc
    28801 acccaggtga acccctgctg ctgtgacagt tcgatcgctt tagctttcgc ttcgtcaaag
    28861 ttcgcgccat gaagcagcac ttcgccgccg aatccacgta ccgcatcgac tttgatgtcg
    28921 gcggtggcgg ttggcataac gatcagggcc ttcacgccta accgcgcaga agaaaacgcg
    28981 acgccctgcg cgtggttacc cgcagaagca gtgatcacgc cgtgcgcttt ttgttcttcc
    29041 gtcaggcccg ccatcatggc gtatgcgccg cgcagcttaa agctgtgcac tggctggcga
    29101 tcttcgcgct tcaccagaat gacgttatca agacgcgacg acagtttttc cattttttgt
    29161 agcggcgtaa cctgcgccgc ctcgtaaacc ggcgcgcgca gcactgctct taaatattcg
    29221 gcaccttccg gagcaccgga caggggttgc gagtcagcca ttattaaccc cccagtttcg
    29281 atttatcgcg caccgcacct ttgtcggcgc tggttgccag gctggcgtag gcgcgcaggg
    29341 caaaggaaac ctgacgttca cggtttttcg gcgtccaggc tttgtcacct cgagcgtcct
    29401 gcgcttcacg acgcgccgcc agttcggcat cgcttacctg taactgaatg ccacggttcg
    29461 ggatgtctat agcgatcagg tcaccatctt caatcaggcc aatgctgccg ccgcttgccg
    29521 cttccggtga gacgtggccg atggaaagac cagaggtgcc gccagagaaa cgaccgtcgg
    29581 tgatcagcgc acaggcttta ccgagaccca ttgatttcag gaagctggtt gggtagagca
    29641 tttcctgcat gccagggccg cctttcgggc cttcatagcg gatcaccacc acatcgcccg
    29701 cgacaacttt gccaccgagg atcgcttcta ccgcgtcgtc ctggctttcg tacactttcg
    29761 ccgggccggt gaatttgagg atgctgtcat cgacgcccgc ggttttaacg atgcagccgt
    29821 tttctgcgaa attaccgtag agcaccgcca ggccgccgtc tttgctgtag gcgtgttcca
    29881 gcgagcggat acagccattg gcgcgatcgt catcgagaga atcccaacgg caatcctgcg
    29941 agaatgcctg tgtggtacga atgcctgcag gacctgcgcg gaacatattt tttaccgcgt
    30001 catcctgggt cagcataacg tcgtattgtt ccagcgtttg cggcaacgtc aggccaagta
    30061 cgtttttcac atcacggttc agtaaccccg cgcgatccag ttcgccgaga ataccgataa
    30121 caccaccagc acggtgaaca tcttccatat ggtatttctg ggtgctcggc gcaactttac
    30181 acagctgtgg aaccttgcgg gaaagcttat cgatatcact catggtgaag tcgatttccg
    30241 cttcctgcgc cgccgccagc aggtgaagta cggtgttagt cgatccaccc atcgcgatat
    30301 ccagcgtcat ggcgttttca aacgccgctt tgctagcaat attgcgcggc agtgcacttt
    30361 cgtcgttttg ctcgtagtaa cgtttggtca attcaacaat gcgtttaccg gcattgagga
    30421 acaactgctt acggtcggcg tgggttgcca gcagcgagcc gttgcccggc tgcgacagac
    30481 ccagcgcttc ggtcaggcag ttcattgagt tagcggtaaa catcccggag caggagccgc
    30541 aggtcggaca cgcggaacgt tcaacctgat cgctctggga gtcagaaact ttcgggtctg
    30601 cgccctggat catcgcatca accagatcga gtttgattat ctgatcggac agtttggttt
    30661 tcccggcctc catcgggccg ccggaaacaa agatcaccgg aatattcagg cgcagggaag
    30721 ccatcagcat ccccggggtg attttgtcgc agttagagat gcagaccatg gcgtcggcgc
    30781 agtgggcgtt gaccatatac tcaacggaat cagcgatcag ttcgcgagat ggcagtgaat
    30841 aaagcatccc cccgtggccc atggcaatcc catcatccac cgcaatggtg ttgaactctt
    30901 tggcaacgcc gccagccgct tcaatttgtt cggcgaccag tttaccgaga tcgcgcagat
    30961 ggacgtgacc cggtacaaat tgggtgaacg agttcacaac cgcgataatc ggcttgccaa
    31021 aatcggcgtc ggtcattccg gtggcgcgcc acagcgcacg agcacccgcc atattacgac
    31081 catgagtggt ggtggcggaa cggtacttag gcatacttta tttactccca gtgtctgtct
    31141 cgtaaatggg acggtgcgtg ccgtcccatt ttttgtattt attgattaac ttgatctaac
    31201 cagccccatt tatcttcggt ttcgccagtg aagaggccga agaaggcttg ctgaatgtgt
    31261 ttggtaaccg ggccacaacg gccttcgcca acctgaatac catctacact gcgcactggc
    31321 gtgatttctg ccgcagtacc ggacataaac acttcatccg ccaggtacag ggattcgcgc
    31381 gacagaacct gctcacgcac ttcaattccc agctctttcg ccagtttgat gatggcatca
    31441 cgggtaatac ccggcagcgc ggaggaggtg aacggtgggg tgaacagcac accatctttc
    31501 acttcaaaca ggttttcgcc tgcgccttca gagatataac cgttcacatc cagcgcgata
    31561 ccttcctgat aaccgtggcg gcgcgcttcg ctacccacca gcagggaaga gagatagtta
    31621 ccaccggctt ttgccgcggt tgggatggtg tttggtgctg cgcggttcca ggaggaaacc
    31681 atcgcatcga tcccctgctc cagcgcttct gcgcccagat acgctcccca cgggaaagcg
    31741 gcgataatca catcggttga gtatcccgct ggcgggttta cacccatgcc aacatcacca
    31801 acgaagatca gcggacggat ataggcgctg gtgagattgt ttttgcggat cacgtcacga
    31861 caagcttcca tcagctcatc aatgctctgc gaaaccggga agcgatagat tttggcggag
    31921 tcatgcagac gctgcatatg ctcacgatgg cggaatacaa ccggtccttt gtgcgagtcg
    31981 tagcaacgga tgccttcaaa aaccgaagtg ccatagtgca gcgcgtgcga catcacatgc
    32041 accttcgcgt cttcccagcg aaccatctcc ccattgaacc aaatgtaatc agctttcttc
    32101 gtggtcattt ttatattcct tttgcgctca ggcgcggatt tgttgtgatg tggttgtgct
    32161 ctggcagatg gcaacgtgtg cgacgtccac cagtttattt aactgactaa acagtaagtc
    32221 gaccgaccgt gggctggcaa cggtcaattc gatatttata ttttgtgcat cgctggcggc
    32281 agccatattc attgagcaga cgtggaaacc acgatgacgc accacgcgta aaacacgttc
    32341 taaggtttcc ggattgaagc gagccgatac attgacctga tgttgcatca tgataatttc
    32401 tccaacattt ctgaattact ggcgccaggc ggcaccagcg gccagacgtt ctcaagttcg
    32461 tcgattgaga catgaagcag gtatggccca tcactgttca gcatggtgtc gagtgccgct
    32521 tcaacctggt ctttacgggt gatgtgttgg ccagggatgc cgaaggcgct ggctaacatg
    32581 aggaaatcgg ggttatcagt aagggtggtt tcgctgtatc gttcctgaaa aaacagttgc
    32641 tgccattgtc gaaccatccc taaccgttgg ttatcgagta agacgatttt caacggtaac
    32701 tgcttgcgtt ttacggtgcc cagctcttgc acattcatca tgaaagagcc gtcaccggag
    32761 atacagacga cagtatcgtt cggtcgtgcg acttgtgcgc caaccgccgc tggtaaaccg
    32821 aaacccatgg tgcctaagcc gctggaggta atgaaatttt ccgggcgagt gtgtgcgatg
    32881 tgctgcgcgg cccacatctg gtgctgcccc acatctgtgg tcacgacgca atccgcaggt
    32941 ttacgatccg acagttgttt taacaacaac ggcgcgtaga tagcgtcacc gggatggtcg
    33001 taacgccagg tatgttcatc acgcagctgc gcgcagtatt gctgccagtc attgatattt
    33061 aacggctgct gtaatgctgg taacagagca tttaaatcac cttgtaatgc cacatgtgcc
    33121 tgacgcagct tgttcatttc tgccgggtcg atatccatat ggataacact ggcgtgtggt
    33181 gcgaaggtgt tcagtttgcc ggtcacccgg tcatcaaaac gtgcacccac ggcgatcagc
    33241 aagtcgcact cctgcaccgc gaagttcgcc gctttggtgc catgcattcc cagcatgccc
    33301 aggtagtacg gataatctgc ttcaactgcg cccagccctt tcagcgtgca ggtggcaggc
    33361 atttttgtgg tagcgagaaa ttctcgtaaa gcaggaactg cctgcgccat acccacgcca
    33421 ccaccaacgt acagcatcgg tttttgcgct tttgccagca tctggcgcgc ttgctcaact
    33481 tcggcatgtg ggaaagtcac ttcgttttca acggtggtga accacggttc caggtcaccg
    33541 ctggctagct ggatatcttt tgggatatcg accagaaccg gaccaggacg acctgagctg
    33601 gcaacgtcga atgctgcagc catgatgcgc ggcaactctt ccagcgactg caccagaaag
    33661 ctgtgcttgg tacaggctaa cgacaatccc aggacatcca cttcctgaaa tgcgtcggtg
    33721 ccgataaacg gtgcggacac ttgaccggtg atggcaacga cagggatgga atctaacagt
    33781 gcgtccgcaa gcccggttat caggttggtt gcgcccggac cagacgtggc gatacatacg
    33841 ccagttttac cggtagcacg ggcataaccg atagccgcca ttgccgcacc ctgctcatgt
    33901 cggcacagca agtgctccac gccgccgtca tacaatgcat cgtaaaccgg cataattgcg
    33961 ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc gcaacgcatg taccacccac
    34021 tgtgcgccat tcatagttag ttccccgtcc tgaatcttga gaaacagaat tttgtgctgt
    34081 tattcattgt ctgctcctcg gttatgtttt taaggtcaaa aaaaaccccc ggacctttcg
    34141 gtgcgggggt cttagttcgt taaggcttga tttctaagcc tttcctcgtc caagtgcagc
    34201 cccgcacggt gggataataa tcaccaccac gctaatcacg accaggctaa tcactcgtag
    34261 aagggctgtc attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca
    34321 tagattttgc gaatagcaca agatattttt tggccgattt ttatgatggc tttaataatt
    34381 tcaatagaaa ttgttgtttt ttaaaaagaa aatagtgagt gaaaaaaatt caatttcagt
    34441 ataaatgttg ttcccttcct cgtagttcat cctttgtccg ctgcattttt gcgagcatca
    34501 ttccaccgcc agaacaactc aacttcactt gcacaaataa acaggaatca gccactaaaa
    34561 ttcacgaatt agcgggctac agcagcgata tcttatgcac ataatggtga ctcaaggagg
    34621 gcttatgtca ctgtcaattg ttcatacccg cgcagccctg ggagtaaatg cgcccccgat
    34681 cactgttgag gtacatatca gtaaaggtct acccggctta acgatggtgg gcttaccaga
    34741 aacaacggta aaagaagctc gcgatcgcgt gcgcagcgcc attatcaata gcggatatga
    34801 atatccggcg aaaaaaatca ccatcaacct ggctccagcc gatctgccaa aagaaggggg
    34861 acgatatgat ttacctatcg ccattgcgtt gctggcggcc tcagaacagc ttacagccaa
    34921 taagttagat gaatatgaat tagtcggaga actggctctt acaggcgctc tgcgtggcgt
    34981 tcccggtgca atctccagtg caactgaagc tattaagtcg ggcagaaaaa ttatcgtcgc
    35041 gaaagataac gaagatgaag tggggctaat taacggtgaa ggatgcctga tagccgatca
    35101 tctgcaagct gtctgtgcgt ttctggaagg taagcacgct ctcgaacgcc cgaaaccaac
    35161 tgatgcagta tcccgggcgc tacaacatga tctcagtgat gttgtcggtc aggaacaagg
    35221 aaagcgagga ctggaaatta ccgccgctgg cgggcacaac cttttactga ttgggccgcc
    35281 gggaacaggt aaaacaatgc tcgccagccg tattaatggc ctgttgccag atttaagcaa
    35341 tgaagaggca ctggagagcg ctgcgatatt aagtctggta aatgctgaat cagtacaaaa
    35401 acaatggcgg cagcgcccgt tccgctcacc tcatcacagt gcgtcgttaa ctgcgatggt
    35461 gggcggtggt gcaattccag ggccaggtga aatttcgttg gcgcataacg gcgtgctttt
    35521 tcttgatgag ctacctgaat ttgaacggcg tacactggat gccttgcgag agccgattga
    35581 atccgggcag atccatcttt cacgcacacg agcaaaaata acctatccag cccgtttcca
    35641 gcttgttgcg gcgatgaatc ccagccctac cggacattat cagggaaacc ataaccgctg
    35701 cacgccagaa cagacattac gttatctcaa ccggctctcg gggccctttc tcgaccgctt
    35761 cgatctctca ctggagatcc cattaccacc ccccggcatt ttgagtaaaa cggtagtgcc
    35821 gggagaaagc agcgccaccg ttaaacaacg cgtaatggcc gccagagagc gccaatttaa
    35881 gcggcagaat aaactgaatg cctggctgga tagtccggaa atacgccaat tctgcaaact
    35941 tgagagcgaa gatgcgatgt ggttggaagg aacactgatc catctggggt tatcgattcg
    36001 tgcctggcag cggttattga aagttgcacg aaccattgct gatattgatc agtctgacat
    36061 tatcacacgt cagcatttgc aggaggcagt tagctatcgt gcgattgacc gtttgctcat
    36121 ccatctgcaa aaactactga cataaaaaaa gggcatttcg ccctttttat taatcatcag
    36181 aatcggtgta gtcttcagca ccttcaacct gcggtttacc gccggaaagg gtgtgaaaac
    36241 gttttggacg cttaatacgc gtcatatact tggaccacac gcgttctgct tctgtcactg
    36301 gctcacgttc gccacggcat actgctacga agagtttctc ttcctcggta accggctcgc
    36361 gtttgccaag atccaactca ttgaaggcat aaccatgacg ctcaagcagt tgtgcctctt
    36421 tgatggtgaa atcaccatga cgagagaatc cacgtggata atgtttattg tcgaaatatc
    36481 gattagtcgt cgtaaagctt tccgccatcc tgcacgctcc taattctttg accgagctag
    36541 ttatggcgcg gagtattagt tacgcttgac agagtgtaaa acaaaacatt taaatcataa
    36601 cgacaaataa ttttgcggag agcactgtgg atacggaatt gttaaaaact ttcctggaag
    36661 ttagccgaac gcgtcacttt ggtcgagcgg ctgaatcgct ctatctgacc cagtcagcag
    36721 tgagctttcg aatcagacaa ctggaaaatc aactgggtgt gaaccttttc acccgccaca
    36781 gaaacaatat ccgtttaacc gctgccggtg aaaaactact gccttatgca gaaacgctca
    36841 tgagcacctg gcaggccgcc cgtaaggagg tggcgcatac ctcacgacat aacgagtttt
    36901 ctatcggtgc cagcgcctcg ttgtgggaat gtatgcttaa tcagtggctg ggacgcttgt
    36961 atcaaaatca ggatgcccat acaggcttac agttcgaagc gcgaattgcc caacggcagt
    37021 ctctggtaaa acagctgcat gaacgccagc ttgatcttct tattaccact gaagcgccca
    37081 aaatggacga atttagtagt cagttgctgg gatatttcac tttagcgctt tataccagtg
    37141 ccccttcaaa actaaaggga gatcttaatt atctgcgact tgagtggggg ccagattttc
    37201 aacagcatga ggcaggtttg atcggtgctg acgaagtgcc cattctgaca accagttctg
    37261 ctgaactggc acagcaacag attgcgatgc ttaatggttg cacctggcta cccgtcagct
    37321 gggcgcgtaa aaaaggcggc ctgcataccg ttgtcgatag cacaacactt tcacggccgc
    37381 tttatgccat atggctgcaa aatagcgata aaaatacgtt gattcgcgat cttttgaaaa
    37441 ttaacgtgct ggatgaagtg tattaatatg aatggctggc aaggatgccg gtagaaggat
    37501 ttacttcgga gagggttatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
    37561 ctggcagggg cggagagact cgaactccca acacccggtt ttggagaccg gtgctctacc
    37621 aattgaacta cgcccctaat tagggtggcg gaacggacgg gactcgaacc cgcgaccccc
    37681 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
    37741 caccggtttt atgaccggct tactgcttaa tttgatgcct ggcagttccc tactctcgca
    37801 tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
    37861 tcaggtggga ccaccgcgct acggccgcca ggcaaattct gttttatcag accgcttctg
    37921 cgttctgatt taatctgtat caggctgaaa atctttctct catccgccaa aacagcttcg
    37981 gcgttgtaag gttaagcctc acggttcatt agtaccggtt agctcaacgc atcgctgcgc
    38041 ttacacaccc ggcctatcaa cgtcgtcgtc ttcaacgttc cttcaggact ctcaaggagt
    38101 cagggagaac tcatctcggg gcaagtttcg tgcttagatg ctttcagcac ttatctcttc
    38161 cgcatttagc taccgggcag tgccattggc atgacaaccc gaacaccagt gatgcgtcca
    38221 ctccggtcct ctcgtactag gagcagcccc cctca
//
LOCUS       DAPGWD010000030        37915 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPGWD010000030 DAPGWD010000000
VERSION     DAPGWD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.030"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(87..374)
                     /locus_tag="RHX88_001470"
     CDS             complement(87..374)
                     /locus_tag="RHX88_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDV2230171.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(449..592)
                     /gene="mgrB"
                     /locus_tag="RHX88_001471"
     CDS             complement(449..592)
                     /gene="mgrB"
                     /locus_tag="RHX88_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDV2230172.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            751..990
                     /locus_tag="RHX88_001472"
     CDS             751..990
                     /locus_tag="RHX88_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230173.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1133..1924)
                     /gene="kdgR"
                     /locus_tag="RHX88_001473"
     CDS             complement(1133..1924)
                     /gene="kdgR"
                     /locus_tag="RHX88_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDV2230174.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2101..3474
                     /locus_tag="RHX88_001474"
     CDS             2101..3474
                     /locus_tag="RHX88_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2230175.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMMAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3520..4401)
                     /gene="htpX"
                     /locus_tag="RHX88_001475"
     CDS             complement(3520..4401)
                     /gene="htpX"
                     /locus_tag="RHX88_001475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDV2230176.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4593..6641)
                     /gene="prc"
                     /locus_tag="RHX88_001476"
     CDS             complement(4593..6641)
                     /gene="prc"
                     /locus_tag="RHX88_001476"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDV2230177.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6661..7359)
                     /gene="proQ"
                     /locus_tag="RHX88_001477"
     CDS             complement(6661..7359)
                     /gene="proQ"
                     /locus_tag="RHX88_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDV2230178.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7456..7953)
                     /gene="msrC"
                     /locus_tag="RHX88_001478"
     CDS             complement(7456..7953)
                     /gene="msrC"
                     /locus_tag="RHX88_001478"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDV2230179.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8083..9366
                     /gene="yebS"
                     /locus_tag="RHX88_001479"
     CDS             8083..9366
                     /gene="yebS"
                     /locus_tag="RHX88_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDV2230180.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9335..11968
                     /gene="yebT"
                     /locus_tag="RHX88_001480"
     CDS             9335..11968
                     /gene="yebT"
                     /locus_tag="RHX88_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDV2230181.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12048..13487
                     /gene="rsmF"
                     /locus_tag="RHX88_001481"
     CDS             12048..13487
                     /gene="rsmF"
                     /locus_tag="RHX88_001481"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDV2230182.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13605..13841
                     /locus_tag="RHX88_001482"
     CDS             13605..13841
                     /locus_tag="RHX88_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDV2230183.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13946..14137
                     /locus_tag="RHX88_001483"
     CDS             13946..14137
                     /locus_tag="RHX88_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDV2230184.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14138..14794)
                     /gene="pphA"
                     /locus_tag="RHX88_001484"
     CDS             complement(14138..14794)
                     /gene="pphA"
                     /locus_tag="RHX88_001484"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV2230185.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15190..15531)
                     /locus_tag="RHX88_001485"
     CDS             complement(15190..15531)
                     /locus_tag="RHX88_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDV2230186.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15544..16416)
                     /gene="copD"
                     /locus_tag="RHX88_001486"
     CDS             complement(15544..16416)
                     /gene="copD"
                     /locus_tag="RHX88_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDV2230187.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16420..16794)
                     /gene="yobA"
                     /locus_tag="RHX88_001487"
     CDS             complement(16420..16794)
                     /gene="yobA"
                     /locus_tag="RHX88_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDV2230188.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16933..17163
                     /gene="holE"
                     /locus_tag="RHX88_001488"
     CDS             16933..17163
                     /gene="holE"
                     /locus_tag="RHX88_001488"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDV2230189.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17265..17921
                     /locus_tag="RHX88_001489"
     CDS             17265..17921
                     /locus_tag="RHX88_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDV2230190.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17945..18607
                     /gene="exoX"
                     /locus_tag="RHX88_001490"
     CDS             17945..18607
                     /gene="exoX"
                     /locus_tag="RHX88_001490"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDV2230191.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18604..20664)
                     /gene="ptrB"
                     /locus_tag="RHX88_001491"
     CDS             complement(18604..20664)
                     /gene="ptrB"
                     /locus_tag="RHX88_001491"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDV2230192.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20873..21532)
                     /locus_tag="RHX88_001492"
     CDS             complement(20873..21532)
                     /locus_tag="RHX88_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDV2230193.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21859..22215)
                     /locus_tag="RHX88_001493"
     CDS             complement(21859..22215)
                     /locus_tag="RHX88_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDV2230194.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22282..22572)
                     /gene="yebG"
                     /locus_tag="RHX88_001494"
     CDS             complement(22282..22572)
                     /gene="yebG"
                     /locus_tag="RHX88_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDV2230195.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22706..23884
                     /gene="purT"
                     /locus_tag="RHX88_001495"
     CDS             22706..23884
                     /gene="purT"
                     /locus_tag="RHX88_001495"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDV2230196.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHIINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23940..24581)
                     /gene="kdgA"
                     /locus_tag="RHX88_001496"
                     /gene_synonym="eda"
     CDS             complement(23940..24581)
                     /gene="kdgA"
                     /locus_tag="RHX88_001496"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDV2230197.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24618..26429)
                     /gene="edd"
                     /locus_tag="RHX88_001497"
     CDS             complement(24618..26429)
                     /gene="edd"
                     /locus_tag="RHX88_001497"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDV2230198.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26664..28139)
                     /gene="zwf"
                     /locus_tag="RHX88_001498"
     CDS             complement(26664..28139)
                     /gene="zwf"
                     /locus_tag="RHX88_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDV2230199.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNLL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28477..29346
                     /gene="hexR"
                     /locus_tag="RHX88_001499"
                     /gene_synonym="yebK"
     CDS             28477..29346
                     /gene="hexR"
                     /locus_tag="RHX88_001499"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDV2230200.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29474..30916
                     /gene="pyk"
                     /locus_tag="RHX88_001500"
     CDS             29474..30916
                     /gene="pyk"
                     /locus_tag="RHX88_001500"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDV2230201.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31047..32018)
                     /gene="lpxM"
                     /locus_tag="RHX88_001501"
                     /gene_synonym="msbB"
     CDS             complement(31047..32018)
                     /gene="lpxM"
                     /locus_tag="RHX88_001501"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDV2230202.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32138..33460)
                     /gene="mepM"
                     /locus_tag="RHX88_001502"
     CDS             complement(32138..33460)
                     /gene="mepM"
                     /locus_tag="RHX88_001502"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDV2230203.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33476..34408)
                     /gene="znuA"
                     /locus_tag="RHX88_001503"
     CDS             complement(33476..34408)
                     /gene="znuA"
                     /locus_tag="RHX88_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDV2230204.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34487..35242
                     /gene="znuC"
                     /locus_tag="RHX88_001504"
     CDS             34487..35242
                     /gene="znuC"
                     /locus_tag="RHX88_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDV2230205.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35239..36024
                     /gene="znuB"
                     /locus_tag="RHX88_001505"
     CDS             35239..36024
                     /gene="znuB"
                     /locus_tag="RHX88_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDV2230206.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36171..37181)
                     /gene="ruvB"
                     /locus_tag="RHX88_001506"
     CDS             complement(36171..37181)
                     /gene="ruvB"
                     /locus_tag="RHX88_001506"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDV2230207.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37190..37801)
                     /gene="ruvA"
                     /locus_tag="RHX88_001507"
     CDS             complement(37190..37801)
                     /gene="ruvA"
                     /locus_tag="RHX88_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDV2230208.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATNDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9070 a         9795 c         9358 g         9692 t
ORIGIN      
        1 tgaagcagtt gcaccatggc agtgcgtata atcaggatga attgagtaac ccttcaacga
       61 cggggttact caagattgag gaaggattat cgttcggcga ccagacgaat aatatcttcg
      121 tcttcaaccg atttttgact ctcaacggtc tttttatctg ctttctcagg ttcgtttgct
      181 tcgcacaaac gacgtaacag tgcattttga cgtttttgct gatccagcaa cgcctcaagc
      241 agttcaatct gttcgttagt ccgagaactg gcacgattga taaaaaacca caagatgagc
      301 ccgataagaa gaaccaccac cgatacaacc aaagacgcaa tgttcatcac gcctgaattt
      361 acaacttcgt tcatttcacc acctctgagt agaggcgcta ttctaccact gctggagagg
      421 aagaaaatct agtgctgaaa aaatgatatc accacgggat aaactggtta atggcacaaa
      481 ttccgctgaa aaattgtaca tcctgatcgc acatcatgtt gaatacctgc gcccaaagca
      541 gcaagcaagc caacaccacg acaaccagaa cgacccatcg aaactttttc actccaatct
      601 ccgcttcacc taccttatgt cattaaacta gcatgcgtta gttaaacagc gcctaactat
      661 gtcgatattc gtgctttttc ggaatgagta gcttgcttca gcgcatattg ccgctacgat
      721 taagcgaaca taaaaagaga agaggttgta atgcgattca tcattcgcac agttatgctg
      781 atagcactgg tatggatagg attattactc agcggatatg gcgttttgat tggaagcaaa
      841 gagaatgccg caggattagg tttgcaatgt acctatctga ctgccagagg caccagcacg
      901 gtacaatact tgcacactaa aagtggtttc ttggggataa cagattgccc actgttacgt
      961 aaaagcaata tcgtggtcga taatggttaa gggtaataaa taaccagatt ctcactcatg
     1021 agtaaaatat tcttatagca agcgcgttta atataacgca ccttcaactt tatttttctc
     1081 atgctaaata aagaacctgt ctgtttgata ttccgacagg ttcttactct catcagaacg
     1141 gatagtcgtg ataacccatt tgggcagaaa ttttgcgcgc tgcggtatgc aacattgcga
     1201 catattcctg taaacgctct tcagagaaac gcaacgtcgg gaaggagatg ctcaaaccgg
     1261 caatgaccac gccaaagcga tcaaataccg gtaccgcaat gcatcgcagc ccttcttcct
     1321 gctcttcgtt atcttcgcca tacccctgct cgcgcacctg gtccagaacg ggtaataacg
     1381 cttctgtact ggtgatggtc cgctcggtac tgcgtttata ctccacgccc tcaagaattt
     1441 gcttcacttc atcgagatcg cgccatgcca gcagtacctt accaatcgcg gtgctgtaca
     1501 gcggattacg acgcccaatc cgtgaataca tgcgcaaatt gtacatagag tcaattttgt
     1561 gaatgtaaac aatactgtct tcgtccagtg cgccgaggtg gatagtttct ttggtcaggc
     1621 gggagatctc acgcatctgg atatctgcgc tacgaattaa atcgacgttt tgtaacgcgc
     1681 gagcgcccag ttcaaacaat ttcagggtca gggaatattt ctccgactcc ccttcctgcg
     1741 ccacataacc taaggttttc atggtctgta aaaagcgata aacggtgctt tttgacatca
     1801 tgacgcgctg cgacagctcg gttatcccta tttcgcgctc ttcacccagc gcctgcaaaa
     1861 tgccaaaaac ttttagcacg gaagatacag aatcaggctg tttatccaga tctgcgttag
     1921 ccatttatca cctcattgcg agtgttttat aaaaatcaga actgtttttt attataattt
     1981 cgcaccaggg tggtcgcaat ccatcttttg ccggttagtt acaattctgc gacatccacc
     2041 gtgaatatca gtgctagaat catacccctg ttgattattc accaaagata taaaattcct
     2101 atgccaaaag ttcaggccga cggcctgcca ttgccccagc gatacggtgc gatattaacc
     2161 attgtgattg gcatttcgat ggctgtcctt gacggcgcaa tcgccaacgt cgccctgcca
     2221 acaatcgcca cggaccttca tgccacgcca gccagttcca tctgggtagt gaacgcctat
     2281 caaatcgcca ttgtcatctc cctgctctcg ttttcgtttc tgggcgatat gtttggctat
     2341 cgacgtattt ataaatgcgg tctggtcgtt tttctgttgt cttcactgtt ctgcgccctt
     2401 tctgattcgc tgcaaatgct cacccttgcg cgtgtcatac aaggtttcgg cggtgcagcg
     2461 ttgatgagcg ttaataccgc acttatccgc ctgatctatc cacaacgttt tctggggaga
     2521 gggatgggca taaactcgtt tattgttgcc gtctcttctg ctgccgggcc gacaattgct
     2581 gcagcaatcc tctccatcgc atcctggaaa tggttatttt taatcaacgt accgttaggt
     2641 attatcgccc tgcttctggc gatgcgtttt ctgccaccca atggttctcg cgccagtaaa
     2701 ccccgtttcg acctgcccag cgccgtgatg aacgcgttaa ccttcggcct gcttatcact
     2761 gcgttgagtg gtttcgctca ggggcaatcg ctgacgttaa ttgctgcgga actggtggta
     2821 atggttgttg ttggtatttt ctttattcgc cgccagcttt ctcttcccgt accgctgcta
     2881 ccggtggatt tactgcgtat cccgctgttt tcactttcta tttgcacatc tgtttgctct
     2941 ttctgcgcac aaatgatggc aatggtttcc ctgccctttt acctgcaaac cgtgctcggg
     3001 cgtagtgaag tcgaaacagg tttacttctg acaccgtggc cgttagcaac gatggtgatg
     3061 gctccgctgg caggctattt gattgaacgc gtacatgcag gattgctggg tgctttaggg
     3121 ttgttcatca tggctgcggg gcttttttcc ctggttctgc tgcccgcgtc acctgcggat
     3181 atcaatatta tctggccgat gatcttatgt ggcgctggat ttggcttatt ccagtcaccc
     3241 aataaccaca ccattattac ctccgctccg cgcgaacgta gcggtggggc cagtggcatg
     3301 ttaggaacgg ctcgtctact gggtcagagt agtggcgcgg cgctggtggc gctgatgcta
     3361 aatcagttcg gtgataatgg tacgcacgtt tcgctgatgg ctgcggctat tctggcagtg
     3421 attgctgcct gtgtgagtgg tttacgtatc actcagccac gatccagggc ataaaaaaag
     3481 cgcgtcgatc aggacgcgct ttttagtatt tacttcatat tacttcaggt attcacccgt
     3541 acgcagagct tcaattcgtt tatccagcgg cgggtgagtc atgaacaact cactgagcga
     3601 tttcgactta ccgttaatgc agaaagccat catgctggtt gcttcttgcg gttcatagct
     3661 ggttttcagg cgctgcagcg cggcaatcat tttctcgcga ccaaccagtt ttgccgaacc
     3721 ggcatcagca tggaattcac gatgacgcga gaaccacatg gtgataatgc tcgccagaat
     3781 accaaacacc agttccagaa ccgttgcaac cgcaaagtag atcagcgggt tgccgttgct
     3841 ctcttcacct tcatcacgat ttccgcccat aaaacccgcg gcaagctgcg ccagaatacg
     3901 ggaaataaag ataacgaagg tgttcaccac gccctgaatc agcgtcatgg taaccatatc
     3961 accattggcg atgtggctga tttcgtgagc aattaccgcc tcggcttcat ccgggctcat
     4021 gttctgcagc aaaccggtgc tgacagcaac cagagaggca tcacggcgcg caccggttgc
     4081 aaaagcgttg atgtccggcg catggtagat agccacttgc ggcatagcga tccctgcctg
     4141 acgagcctgg gttgctacag tattaaccag ccaacgttcc ctttcgttac gcggttgctc
     4201 gatcacttcc ccgccaacag atcgtaatgc catccatttg gacatcagaa gcgaaacgaa
     4261 ggaaccacca aaaccgaaca gcaaggccat gatcatcagc ccctgaacgc tgctcgactg
     4321 tatccctgtc aggctcagta ccagcccgaa aacgaccatt acggccaggt tcgttagcag
     4381 gaagagcgcg attcgcatca taattttctt tttacctcag tttaacaaaa cgcaatatgc
     4441 gatacccaca tcgtatgggt tacgcgacta ttttcaagtc tggatagtgc gtaagtcacc
     4501 agaaagacac aactttacat tttgtagcat ctgatttacg gcatcttgcc gctgttaaaa
     4561 aatcaggcac aatttcttgt gcctgattga tattacttga cgggagcggg ttgttccgcg
     4621 ggtctggctt tttcaagctt cgccagatcg agtgcgatat tcaccgtctc atccagataa
     4681 ggatccggct cctggtaatc ttttggtaga tcatccagct tcttcaactc cggtttacct
     4741 tcgcgtttaa agcgttcgtt caaacgcgcc agacgcgtcg catcatcttc attattctct
     4801 ttctcacgca cagcgtaatt cagagaaacg atattgcgct tgtccttcat agcgttgaag
     4861 cgcgcgatat ccttcatgat gttctggaac tcaggatctt tcgcgatacg cgcattatgt
     4921 tccttcagta gctccggttc aaaggccgtt aaatcgcctg atttcacata agtcgcagca
     4981 tcaatgctat cccacggcag cgcgttatct tcgaatttct cacccgtttc cgtttcttca
     5041 ttacccgtcg gcatgatgat gtctggcgtt acgcctttac gttgcgtact gccgccgtta
     5101 acgcgataga atttctggat cgtgtactgc acagaaccca gcgctggcca ttcaggacgt
     5161 aacatctgat cgtaaatacg gttcaatgaa cggtattgct gaacagtgcc tttaccaaac
     5221 gtcggttcac ccacaaccag cgcacgaccg taatcctgca ttgccgcggc aaagatttct
     5281 gaagccgaag cactgaaacg gtcaaccagt accaccagcg ggcctttata gaacacctgc
     5341 ccgtcggtat cgctgtcttc acgaaccttg ccgttgttat cgcggacctg aacaatggga
     5401 cccgcaggaa taaacagacc tgagagcgat accgcttcgg tcagcgcccc accgccattg
     5461 ctacgcaggt cgatgatcac actgctgaca ttctgttttt ccagtttctg cagttgaact
     5521 ttgacatcgt ctgtcaaacc cacatagaag cccggaatat ccagcacgcc gactttctct
     5581 ttaccgacgg tcttcaccga cattttaacc gcgcggtctt cgagacgaat acgttcacgg
     5641 gtcaatgtta cagtgcgggt cttggtccct ttaccagcag gtaaaatttc cagacgaact
     5701 ttactgccct tcggcccttt aattaaggca accacatcat caagacgcca gccaatcacg
     5761 tcaaccatcg gcttgcctgt ttgaccaaca ccgacaattt tgtcaccaac gctgatagct
     5821 ttactcttcg ctgccggacc acctgccacc atcgaattga taacggtgta gtcatcatcc
     5881 atttgcagca ctgcgccaat accttccagc gacaaactca tttcagtgtt gaactgttcg
     5941 gtattacgcg gggaaagata gttggtatgc gggtcgattt cacgcgcaaa cgccgtcatt
     6001 gccagcgaga aaacatcttc gctgttggtt tgcgccagac gacgaatggc aaatttgtag
     6061 cggcgtgtca gggtttcacg aatttcttta tccgtttttc ctgccagctt caggcttaac
     6121 tcgtcgaatt tgactttact gtcccacagc gcgttcaact cagcctcgtt tttcggccag
     6181 ggcgctttgc tgcggtcaag gttataagtg tcgttgccgg tgaaatccat cggcttttcc
     6241 agtaccgaca aagcgtactg gtaacgctca aaacggcgct tttgcgccag attgtagaga
     6301 tcgtagaaaa cgtcgagttt gcctgaacgc agttcatcgc ctaactcggt tttctttttc
     6361 gcgaactgtt caacatcgct tgccagcagc acgttgtggc tgtaatcgag cagattcagg
     6421 tagcggtcaa agattttggc cgaaaatgcc tgatcgaggt cgaactggcg ataatgagaa
     6481 cgggtgaagc gcgacgttac gcgctcactt accgtcgcat gctgcgtctc ttcctttaat
     6541 accggaattt gatcagcacg cgtgatatct tctacagcga aggtctggcc tgctattgca
     6601 agcaggccag ctaacgcggt aagcctaaaa aacatgttca tgcctggccc ggcctccgtt
     6661 tcagaacacc aggtgttctg cgcgcacaat caaagacata cccgaattca gctggacgcg
     6721 gacgccgtct ttggtgattt ctaatacggt ggcatccatc gcgttttgac ccgctttcac
     6781 cttcagggct tgtccgacag tcagagctga aatgtcagaa accggggtgt gctgttcttc
     6841 gcgaggtgct tttactgttt ttggcgcttt ctctaccggc ttttgcgcac gaggtttacg
     6901 ttcagcgcct tctttgcggc gtggcgtggt cggacgtggc ttgcgttcgc ggcgcggtgc
     6961 gtcttctttc tcaccagcag ctgcggcagc ttcgcgtttt ttcgcttgct gttcagcacg
     7021 ctgtgcctga acacgcgctt tcgcttcttc aagctgcttg cgagcatgct ctacatgttg
     7081 ctcgtccagc tcaccgcatg ggttgccgtc aagatcgaca cgcgttgcgc cgggtttaac
     7141 accgtaaaga taacgccagc tcgaagtgta gagacgtaaa gcggatcgta attgcgtttt
     7201 gctcaggttc atttccccgg caacacgatc gaccaaatcc tgaaaaatac cgattttcag
     7261 cggacgcgct tcaccttccg cactgaaaca gtggggaaaa cgttcggcca gaaacgcgat
     7321 tacttcttta ctgctattca acttaggttg attttccatg aaatttcctg attacaacgg
     7381 acgtagccaa caagcgcagg catgaacagg cgtcattata atgacgctac cagtaaatgc
     7441 tacgttatcc gttgattatc ctgcgacgct cgcaaagaat tttttgtaat ccgtcgttgc
     7501 aagcactttt tcaagctgtg ccacaagctg acgtaagcct tgctcgtcct cgtctgtaaa
     7561 gcgaccgaag acggtactat cgatgtcaag aacaccaata atctgatttt tcaccaccag
     7621 cggcagaaca atttcagaat tactcgccgc atcacaggca atatgcccgt caaacgcgtg
     7681 aacatcctcg atacgctgca cttgattgcg ggcaaccgca gtgccgcaca ccccgcgccc
     7741 gacgggtatc cggacacagg caattttgcc ctgaaatggt cctagtacca gtgtatcgtc
     7801 ctcaagcaaa taaaaacctg cccagtttac gtcagtgaga cgctcgtata acaacgcact
     7861 ggtgttcgca agcgttgcca gaaaactggt ttctcccgcc atcagcgcgt taaagtcgcg
     7921 atttaaatcc gcgtaaaatt ctgttttgtt cattatataa tcacttggtt gtcttacctg
     7981 gaactgccag cctattaaaa taagcattaa atgcgttaat gctcaagatc attcccatca
     8041 tgggttaata ttaatgttaa ttcttattac atttggcacg tcatggctct taacacacca
     8101 caaattacgc cgacaaaaaa gataacagtg agggcaatcg gcgaggaact gccgcgtggt
     8161 gattaccaac gttgcccgca atgtgacatg ctgtttagcc tgcccgagat aaattctcat
     8221 cagagtgcct attgtccgcg ctgtcaggca aaaattcgcg acgggcgcga ctggtcgcta
     8281 acgcgcctgg cggcaatggc tttcaccatg ctgttgctga tgccgtttgc ctggggcgaa
     8341 ccgctgttgc atatctggct gttaggtatt cgtatcgacg ccaacgtcat gcaaggcatc
     8401 tggcaaatga ccaaacaggg cgatacgata acgggggcga tggtcttttt ctgcgttatt
     8461 ggtgcccccc tcattctggt gtcctccata gcatatttat ggtttggtaa ccgacttgga
     8521 atgaatttac gtccggtact gctgatgctt gagcgactta aagagtgggt aatgctggat
     8581 atctacctgg tcggcattgg cgttgcttct ataaaggtac aggattatgc ccatatccag
     8641 gcgggtgtcg gcttgttctc ttttgtggcg ttggtgattt taacgacggt gacgttgtca
     8701 catcttaatg tcgaggagct gtgggagcga ttttatccgc agcgccccgc tacgcgtagg
     8761 gacgagaaac ttcgtgtctg tcttgggtgc cattttaccg gctatcctga tcagcgtggt
     8821 cgctgcccgc gttgccatat cccgctacgc ctgcgtcgcc gtcatagttt gcaaaaatgc
     8881 tgggcggcgc tgttagcgtc aatcgttttg ttgttacctg ccaacctgtt gcctatttct
     8941 atcatttatc tgaacggagg acggcaggaa gatacaattc tttccggaat tatgtcgctg
     9001 gcaagtagca acattgcggt tgcgggaatc gtgtttatcg ccagtattct ggtaccgttt
     9061 actaaagtga tcgtcatgtt cactttactg ttgagcattc attttaaatg ccagcaaggt
     9121 ttacgcacac gcattctgtt actgcggatg gtgacctgga ttggtcgctg gtcgatgctc
     9181 gacctgtttg tcatatcttt aaccatgtcg ctgattaatc gcgatcagat cctcgctttt
     9241 actatgggac cggctgcgtt ttatttcggc gcagcggtaa ttttgactat tcttgctgtg
     9301 gaatggctgg acagccgctt actttgggat gcacatgagt caggaaacgc ccgcttcgac
     9361 gactgaagcg cagattaaaa ataaacgccg tatctcacct ttctggctgc tgccgttcat
     9421 cgcgctaatg attgccggtt ggctgatttg ggacagttat caggaccggg gtaataccgt
     9481 caccatcgac tttatgtcgg cggatggtat tgtcccgggc cgtacgcctg ttcgttatca
     9541 gggtgttgaa gtcggaacag tgcaggatat cagcctcagc gacgatcttc gtaagattga
     9601 agtcaaggtc agcatcaagt ccgatatgaa agatgcgctg cgcgaagaga ctcagttctg
     9661 gctggtgacg ccaaaagcat cgttggcagg tgtctccggg ctggacgccc tcgtcggtgg
     9721 taactatatc ggcatgatgc caggtaaagg taaagagcag gatcactttg tcgcactcga
     9781 tacccaaccg aaatatcgac tggacaatgg cgatctgatg atccacctgc aagcccccga
     9841 tctcggttcg ctgaacagcg gttcattggt ctatttccgc aagatccctg tgggaaaagt
     9901 ctacgactat gccatcaatc ccaacaagca aggcgtggtg attgatgtcc tgatcgagcg
     9961 gcgttttacc gatctggtga aaaaaggtag ccgtttctgg aacgtttccg gcgttgatgc
    10021 caacgtcagt gtcagtggcg cgaaggtgaa actggaaagt ctggcggcac tggttaacgg
    10081 tgcgattgcc ttcgattcac ctgaagagtc gaaacctgcc gaggcggaag atacctttgg
    10141 tctgtatgaa gatctggccc acagccagcg tggcgtaata ataaaactgg aactgccgag
    10201 tggggccgga ttgaccgccg actcgacgcc gttaatgtat caggggctgg aagtcggaca
    10261 gctaactaaa ctggatttaa atcctggtgg taaagtcact ggggaaatga cagttgatcc
    10321 cagcgtcgtt accctgcttc gtgaaaatac ccgcatcgaa ttacgtaatc cgaaattatc
    10381 ccttagcgat gccaatctca gcgccctgct gactggcaaa accttcgagt tggtacccgg
    10441 cgatggcgag ccacgcaaag agttcgttgt tgtgccaggc gaaaaagcac tgctgcatga
    10501 acctgatgtt ctgacgctga ccctgaccgc gccggaaagt tacggtattg atgctggtca
    10561 gccgctcatt cttcacggcg tgcaggtagg ccaggttatc gatcgtaaac tcaccagcaa
    10621 aggcgtcacc tttaccgtcg ccatcgagcc tcagcatcga gaactggtaa aaggcgatag
    10681 caaatttgtc gtcaacagcc gtgtcgacgt gaaggtgggg ctggatggcg ttgagtttct
    10741 cggtgccagc gcctcagaat ggattaacgg cgggatacgt attctgccgg gcgataaagg
    10801 cgagatgaaa gccagctatc cactgtatgc caatctggaa aaagcgctgg agaacagcct
    10861 tagcgattta cccaccacaa ccgtgagttt gagtgcagag acgctgccgg atgtgcaggc
    10921 aggatcggta gtgctctacc gtaaatttga agttggtgaa gttattaccg tccgtccgcg
    10981 agctaacgcg tttgatatcg atctgcatat taagccggag tatcgcaacc ttctgaccag
    11041 caatagcgtg ttctgggcag aaggcggggc gaaagttcag ctgaatggta gtggcctgac
    11101 cgtacaggca tccccgctct ccagagcatt aaagggagcc attagcttcg ataacctcag
    11161 cggtgccagc gccagtcagc gtaaaggcga caaacgaatt ctgtatgctt ccgaaacagc
    11221 ggcccgtgcg gttggtgggc agattacgct tcacgctttc gatgccggaa aactggcggt
    11281 cgggatgcca attcgctatc tcggtattga tatcgggcaa atccagacgc tggatctgat
    11341 taccgcgcgc aatgaagtac aggcaaaggc ggtgctctat ccggagtatg tccagacctt
    11401 cgcccgcggc ggtacgcgct tctcggtggt cacaccgcaa atttcagccg cgggcgttga
    11461 gcatctagat actatcctcc agccgtatat caacgtcgaa ccaggccggg gcaatcctcg
    11521 ccgcgacttt gaattacaag aggccaccat tactgattcg cgttacctgg atggcttaag
    11581 cattattgtt gaagcgccgg aagccggttc gttaggtatt ggtacgcctg tgctgttccg
    11641 tggtctggaa gtcggtacgg ttacaggaat gacgctgggg acattgtcag atcgcgtgat
    11701 gattgcgatg cgcatcagta aacgctatca acacctggtg cgtaacaatt ccgtcttctg
    11761 gttggcatcg ggttacagtc tggactttgg tctgacgggc ggcgtagtga aaaccggcac
    11821 ctttaaccag tttatccgtg gcggcatcgc cttcgccacg cctccgggta cgccactggc
    11881 accgaaagcc caggaaggca aacacttcct gttgcaggaa agtgaaccga aagagtggcg
    11941 tgaatgggga actgcgcttc ccaaataatg cccactgctc cggcgtgcct gcgccggagc
    12001 gtttatgcta aactgcgcgc ctgttttttt gccagtggta catgctcgtg gcccaacaca
    12061 ccgtttattt cccggacgcc tttctgacac aaatgcgcga agcgatgcct tcgacgctct
    12121 catttgatga ttttcttgcc gcctgtcagc gcccgttgcg ccgcagcatt cgcgttaata
    12181 cgctgaaaat ctccgttgct gatttcctgc aattaaccgc tccttatggc tggacgctta
    12241 cgccaattcc gtggtgtgaa gaaggtttct ggattgaacg cgacaatgaa gatgcattgc
    12301 cattgggtag taccgccgag catttaagcg gcctgtttta tattcaggaa gccagttcaa
    12361 tgttgcccgt tgccgccttg tttgctgacg ataatgcacc acagcgggtg atggatgtcg
    12421 cagctgcgcc aggctccaaa acgacgcaaa ttgccgcgcg gatgaataac gaaggggcaa
    12481 tccttgccaa tgagttttcc gccagtcggg taaaagtgtt acatgccaat atcagccgct
    12541 gtggcatcag taatgttgcg ctcacacatt ttgatggccg cgtgtttggt gcggcagtgc
    12601 cagaaatgtt cgatgccatt ttgctggacg ctccctgctc tggcgaaggc gtggtgcgta
    12661 aagatcccga tgcgctaaaa aactggtcac cagaaagcaa tcaggaaatc gcagctacac
    12721 aacgggagct tatcgacagc gcctttcatg cattacgtcc tggtggtacg ctggtttact
    12781 cgacctgtac cttaaaccag gaagaaaacg aagccgtttg cctgtggctg aaagagactt
    12841 accccgacgc agtagagttt ttaccacttg gcgatctctt ccctggtgca aacaaagcgc
    12901 tgaccgaaga aggctttttg catgttttcc cacaaattta cgactgcgaa ggcttcttcg
    12961 ttgctcgtct gcgtaaaact caggcgatcc ccgccttacc cgcccccaaa tacaaagtcg
    13021 gtaatttccc gttcagcccg gtgaaagatc gcgaagccgg acaaattcgt caggcggctg
    13081 caagtgttgg cttaaactgg gatggaaacc tgcgactctg gcaacgcgac aaagaactgt
    13141 ggttgttccc ggtgggcatt gaagccctga tcggtaaagt ccgattttct cgcttgggga
    13201 ttaaacttgc cgaaacgcac aacaaaggtt atcgctggca gcatgaagca gttattgccc
    13261 ttgccacccc cgacaatgtg aacgcttttg aactgacacc gcaggaagcg gaggagtggt
    13321 atcgcgggcg cgatgtttac ccgcaagccg cgccagtggc ggatgacgtg ttggttactt
    13381 tccagcatca accgattggt ttagccaaac ggattggttc gcgattgaaa aacagctatc
    13441 cgcgtgaact ggtgcgcgat gggaaacttt ttaccggtaa cgcctgacag cgcacaaaaa
    13501 aagcgcactt tttgactggc acattcggct gcctcaacta ggctgaaaaa tggtgcgatc
    13561 ggactggtcg taccacaacc ggcagctaaa tggagagcac gaagatgaaa accagtgtgc
    13621 gcataggcgc ttttgaaatc gacgacggcg aattacacgg tgaatcgccg ggtgatcgaa
    13681 cgttaaccat tccttgtaaa tctgaccccg atttatgtat gcaactcgat gcctgggatg
    13741 ctgaaaccag tatccctgcc ctgctaaatg gcgaacactc tgtcctttac cgtacccgtt
    13801 acgatcaaca atctgatgcc tggattatgc gtcttgcctg atccaaaaag aacccgtcgg
    13861 catggcgggt tatttgtcct ggttattccc ccgttgtaaa atctctccta aacttaacgg
    13921 tacggcacca cacttcgggg atgaaatgtt cgcgctggta ctttttgttt gctacctgga
    13981 tggcggttgt gaagatattg ttgtggatgt ctacaacacg gaacagcagt gtctttattc
    14041 tatgagcgat caacggatcc gccatggcgg ttgttttccg attgaggatt ttatagatgg
    14101 tttctggcga ccagcacagg agtatggtga tttttaatta ttgcaattgc acaagagtca
    14161 gttcgccccc aaagacagca ccggtatcaa tataatgcag gttgccaata tccacgcgat
    14221 gtcgcaacgg tgtatgacca aaccagaaat gatcagcacc tgtaattccc tgcccttttt
    14281 ggcgttcacc taatcgcgag cggctccaca agacctgatg caaatcaacg tccttttgcc
    14341 attcataaac atcatctgga taatcggcat gagcaataac atgtttgccg gtacgactgt
    14401 gtacttcaag aataaagggc aaatgctgac atttttccag cgccgttttc gcttgtttct
    14461 gttgattatc tgccagcgca ataaaccagt cgccgccatt catcaaccac aaagacatct
    14521 gctgggatgc cagcgcatcc atcgccatct gttcatgatt gcctcttacc gcacgaaccc
    14581 aatgttgttc cagtaactgc aggcaacgta aactttgcgg cccacgatcg ataacgtctc
    14641 ccactgagat aagtaaatct cgccacggat caaaaccaca atgccataat ttgcggcgca
    14701 actgctcaag acaaccgtgt atatcgccag aaagccagat atgtcgccat tgatgacccg
    14761 caattctctg ataaacgggc gcaggctgtt tcatcaatat tttcctcccg cgctaaagat
    14821 cacataatct taacaagaat gttaaaaaac gctggactca gacagtagag tgtgtgttat
    14881 ggttgactat aaagtcagcg aaggaaatgc ttctggcttt taacagataa aaagagaccg
    14941 aacacgattc ctgtattcgg tccagggaaa tggctcttgg gagagagccg tgcgctaaaa
    15001 gttggcatta atgcaggctt agttgccttg ccctttaaga atagatgacg acgccaggtt
    15061 ttccagtttg cgtgcaaaat ggtcaataaa aagcgtggtg gtcatcagct gaaatgttaa
    15121 aaaccgcccg ttctggtgaa agaactgagg cggttttttt attggaaatc aaaaggctat
    15181 tttaggtaat taacagagtt tttcagctcg ttctataaac ggtgccagac tcattttttc
    15241 gccgggattg ttaggatcat caatctgaat caccgaaatg ggttgggctt tagtcttccc
    15301 actggcaact tccttttgtg cgatatcgtt taaaggatac tgcacgaggg tacttgggtt
    15361 gatgacatac aaagcattac ccggtcggca agtcagcatc acctcttcgc gattaaacgc
    15421 ccatttgtct ttacccactt caaaacggct gacggtaatc acctgcggtg cagccagcgc
    15481 cgctgcagaa ctggtgagta acagaaacgc cagaatactt tttttcatca ttttcgtaat
    15541 ccatcaaaaa ggttcgagag tcgcaagcag gctgatgatc accagcacta ccgcacctat
    15601 cgcccattcg agtttagtca tccactcaaa ccagggtgcc gcacgatctt catcctgcct
    15661 catgcgcggt acaagaacat aacgattagc cagcgcaatg accaccataa tcatcaccag
    15721 gatggctttg agcaataaga gttggcccca gtaagtcgta agcgtggggg gaaatccggt
    15781 aatcaacaat gcattaagca cgccgcttgc cagtacgccg atcaccgcaa aatgcccgca
    15841 ccaggaaaaa cgcatcagcg cctgaatggc ctggtgtcgc caacggcctt tgatgagttg
    15901 catacaccag agcaccggca gtaatccccc aaaccacgcc gccgcacaaa tcaggtgaat
    15961 cgcgtgatta gtctgatgga ttttcgccgt taccccttca ttcagtgtcg catgcccgat
    16021 tcccgccagc agaataaatt gcgcggtagt gagcataaac agcaaacgtg gcatattccg
    16081 cggttgcata agggcgacga tcaacgttac gagggcgaga acaatttgcc ataaccagac
    16141 gccaccaaac tgcgtttgca aaacagcctg ccaaatgttt ggcgaaaata catctgccca
    16201 tcccgttccc atcaacccgc cctgaacggc gagcattgca gtggcactga tcaaactcca
    16261 gacggcagcg tgctgttgta aacgcagaaa acgcttcgcc agcaagcgac gaatcgttaa
    16321 gggggccagc caggcaccgt acattgcgaa gccaaagacc agcatcagcg aggtgaaatg
    16381 gataaatcgc agcgcgatcc aggtaaacgc cagcatgatt tatttcacgc taaaggtgta
    16441 gtgtcctttc gttttatgcc cgtccaccga aacaacatgc cagtcaacgg tgtaggttcc
    16501 gggtttcagc gaatcggcca gtggaacaat cagttgcttt tggtcctgct catttcgctt
    16561 cgccggtaat gttttaatat tttcgttttt tggccccgtg atttttgcac cactgaatcc
    16621 tgtttcaaca ccttccgaga agtttaaagt gattgcctgc ggtgcagctg tcacttgcgc
    16681 gtttgccgca ggatactgat gcgttaaatg cgcatgtgcc cagacggaag gtgtcaccaa
    16741 cgaagtggtc aggattgcca gcgcgtagcg aagggagcgt gcagttgaag tcatattatc
    16801 tattcctttt tgtaataact tttttacaga gcataacctt gtctaatgtc tgagtcgagg
    16861 atcatcaatt ccggcttgcc atcctggctc actcttagta acttttgccc gcgaatgatg
    16921 aggagattaa gaatgctgaa gaatctggct aaactggatc aaacagaaat ggataaagtg
    16981 aatgtcgatt tggcggcggc cggggtggca tttaaagaac gctacaatat gccggtgatc
    17041 gctgaagcgg ttgaacgtga acagcctgaa catttgcgca gctggtttcg cgagcggctt
    17101 attgcccacc gtttggcttc ggtcaatctg tcacgtttac cttacgagcc caaacttaaa
    17161 taaaacttat acagagttac actttcttac ataacgcctg ctaaattatg agtattttct
    17221 aaaccgcacc cataatttgc agtcattttg aaaaggaagt cattatgtcg ttctggaaag
    17281 ttgcagcagc gcaatatgag cctcgtaaaa cctcactcac cgaacaggta gctcatcatc
    17341 tggagtttgt gagggccgcc gccaggcaac aatgtcagct gttagttttt ccatcgcttt
    17401 ctttactggg gtgtgattat tcgcgacgtg cccttcctgc cccacccgat ctctcactgt
    17461 tagacccgct ttgttatgcc gcaacgacct ggcgaatgac catcattgcc ggccttcccg
    17521 ttgaatataa cgatcgcttt attcgtggaa ttgcggtgtt cgcaccgtgg cgaaaaacgc
    17581 ctgggatcta ccatcagagt catggtgcat gtctgggtcg tcgttccaga accatcacgg
    17641 tggtcgatga acaaccgcaa ggtatggata tggacccgac ctgttcactg tttactacag
    17701 ggcaatgtct gggagaaccc gacctgctgg cgtcggcccg tcgcttacaa tttttttcac
    17761 atcagtactc gattgccgtg ctgatggcaa atgcccgtgg taacagtgca ctatgggatg
    17821 aatatggtcg tctcatcgtt cgcgccgatc gtggttcatt attgttagtc ggtcagcgtt
    17881 catcacaggg ttggcaaggc gatatcattc cattacgcta ggctttttcg gcctggagca
    17941 tgccatgttg cgcattatcg atacagaaac ctgcggtttg cagggaggga tcgttgagat
    18001 tgcctctgtt gatgtcattg acggaaaaat cgtcaacccc atgagccatc tggtgcgtcc
    18061 cgatcgtcct attagtccgc aagcgatggc gattcatcgc atcaccgaag ccatggtcgc
    18121 cgataaaccg tggattgaag atgtgatccc acactattac ggtagtgaat ggtatgtcgc
    18181 gcataacgcc agctttgacc gccgcgtact gcctgagatg cccggtgagt ggatttgcac
    18241 tatgaaactg gcccgtcgtt tgtggccagg gatcaagtac agcaatatgg cgttatataa
    18301 aacacggaag ctcaatgtac agacgccgcc gggcctgcat catcaccgcg cgttgtatga
    18361 ctgttatatc accgccgcgt tgcttatcga tattatgaac acctccggct ggacggcaga
    18421 acagatggcc gatatcaccg gacgtccgtc gttgatgacg accttcacct ttggcaaata
    18481 ccgtggcaaa gcggtttccg acgttgccga acgcgatccg ggctatctgc gctggttatt
    18541 taataacctg gacagcatga gcccggagct gcgtttaaca ctgaaacatt atctggaaaa
    18601 tacttaatcc gcaggcgtag cgggtaatgt tccctgcgcc agcgcgacca gaaaagcata
    18661 ttccatcgct acgccttcgt acgatttaaa gcgaccagat ttaccgccat ggcctgagtc
    18721 catgtcggta cagagcaata aaagatggtc atcggttttc agctcgcgca atttagcgac
    18781 ccattttgcc ggttcccaat attgcacctg agaatcgtgc aaaccggtcg ttaccagtaa
    18841 atgcggataa gcctgtgcgg tgacgttgtc atacgggctg tagcttttca tgtactcgta
    18901 atattgcgga tcctgcgggt taccccactc ttcaaactca ccagtggtaa gaggaattga
    18961 ttcatcaagc atcgttgtta caacatcaac aaacggtacc tgggcgataa cgccgtggaa
    19021 taattccggg cgttgattaa ttgcaacgcc catcaacatg ccccccgcac tcccgcccat
    19081 cgcataacaa agcgaaggag agccatagcc cagttttaac aatgcatcgc aggcatcaag
    19141 ataatcatta aacgtatttt tcttcttcag aaattttccg tcttcgtacc attgttgccc
    19201 cagctcacca ccgccgcgaa catggacaat ggcgtagaca aagccacgat ctaacaaact
    19261 caagcggcta aaactgaaat cggcatcaat acttgcgccg taagaaccat agccatacac
    19321 cagcaacggg ttgtgtcctt tgcgaaaatg tttgcgatgg tagaccaacg aaaccggaac
    19381 ttcgacgcca tcacgggcga ctatccacag gtgttcactg cggtaattcg ccgcatcaaa
    19441 accgggcact tctgtttgtt ttaatacacg acgctcaccg gtatccatat ccagttcaaa
    19501 caaagtgtct ggtgtggtca tggaagaata accataacgc aatcgcgcgg tttcaggttc
    19561 tggattgtag gcaatccagg tcacataggc tggatcatca aaggcaatac caatgacttc
    19621 ccgggtcttg cggttaattt ggcgcaaact ggttaacccg cgctgacgct cttcaaccac
    19681 cagccagtcg gtaaacagcg taaacccttc cagcatgatg ttttcgcgtg gcggaattaa
    19741 ctcttcccac tgttgctcat cacgcatacg ggtacggtat aagccaaagt ttttgccgtg
    19801 gcggttggaa cgtagataaa aacgatgctg gtagtgatca aggctgtatt cgtgatcttt
    19861 gcggcgcggc agaaaaacaa acggctcggc atcggccatt tccgcgtcca gcaggcgaac
    19921 ttcactggtg gtggcgctgg ccaaatgaat gactacatag tgcttcgagg tcgttttatg
    19981 caggctgacg taataggtat cgtctttttc ttcgtagatc agtttatctt gcgatgctgg
    20041 cgtaccgatg gcgtgacgcc agacctgata aggcagcagc gtcaccggat gcttgcgaac
    20101 atagtagaaa gtccaggagt catttgccca gacaaagctg ggttcaacgt tatccagcag
    20161 ttccgggtac cagttaccag tttccagatt acgaaaacga atgccgtact ggcgtcggga
    20221 aagaaaatct tctgccagcg ccataatggt gttatcgggc gtaatcgcca ttccgcccat
    20281 cgaataaaac tcactatgag ctgcgcgctt attggcatcg agcaatgttt cccactcatc
    20341 ccactcttca ctgaatgccg attgacgctg gtagatagca tattcacagc ctggttcata
    20401 aatatgccga tagcggtagc catttttgat gtagggcgca gaaacttctc gttgcggaat
    20461 gcggtcgatg atttccttta agatgcgatc ctgcaaggct tgttgtgagg ccatcacccg
    20521 atgaccgtaa ctattttctt gttgcaggta gtccagaact tccggctgag aacgcgtatc
    20581 gtcccgcagc cagtagtaat tatcgattcg cgtatcgcca tgaagcgtca tggcgtgggg
    20641 aatgcgggcg gcttttggta gcatgttatt gttctttctg gttgaaacat cttataaggg
    20701 tggcaaaact caccggggat gcaagcgaaa caggggagtc attgcttaga tgatgacagg
    20761 taatggcgcg gatatcgaat gttatgcaga cagagaaaat cagcctgttc aaatggctgt
    20821 gcgattctgg atagcccgaa atagtcaact tcaggctatc cagagagcgg aattattccg
    20881 ccaaagtgcg tttttgctgt tcgagatcgc gttcaatgcc gtcacgaaca tcctggggga
    20941 ttttcagcgc gtcacccagt gcattcaggt aactgcgttc cataaaatgg tcaatatcaa
    21001 tagccgcgca actcaggaaa tagatttcca gcgcctcttc ttcattgcgg accccggtag
    21061 ccaggcgttg tggatccagc ggttgttcga ttgcctgctc aatgagtaca cgcccctgct
    21121 cttccacgcc ggcttcacgc aattgctggt cgattgccgc acgttctttg gcatcaatat
    21181 gaccatcact tttagcggca aagaccagcg caaggatcaa acgttctgta cgctcatcca
    21241 gcggcgtact ttgcgcgcca aactgcggtt cgtcctgatg cgccgcgcga attttatctt
    21301 tgtatttatt ccacagcacc gtacccgcta ccgctccgcc gccaaccagt aacgcgttgg
    21361 tgccatattt cgtaagaagt ttacgtgctg atttatttgc gaccagcagc ccagccagtc
    21421 cgcctaatgc gcctggcact aacagtttgc ccaatccctg atccgcagac gaggaggtag
    21481 aagaactgct ttgcccaagc agggattgca gttgatttaa ccagttagcc atatttgctc
    21541 ctcaataacc atttttagtg cctgtagcat agcggatgag gatgtcagaa aatgtatatc
    21601 gagacaaaag atgcgcaaat aagcggggcg gtaggccgtt attcaaagaa attcgcgcca
    21661 ggtaaggtat ttactgtata tcaggtgata agggtatttt aggtgaaagt agtttgtcgt
    21721 tttgagtggc attgtttgat gctgattgcc cgatgcgacg ctgttgcgtc ttctcaggcc
    21781 tacgattgtg gtgcgttctg gggcggacaa ggcactcgcg ccgcatccgc caacggtcaa
    21841 tcgaattatc agaagccatt aacgccgctg atattccgcc attcccgctt tgcagtccac
    21901 gctgacctgg taatgaatat cggcactttt accacgcacg gttagcggta ctgaccattt
    21961 atcatcttta ccctgaatgt cctgcaaact gacccaagcc acgggatcgg cctgaccgac
    22021 aattttttga tcatctgccc agcgcgccac gcgattttgt tgataatcac gttttacgct
    22081 cgcggcaatt ccggcagcat ccagaccttc acactttggg aaagtgaccg acttgctggt
    22141 ttcattattg gcagcgaaaa ctgatgcgca ggcagaaacc aacaacagcc ctaaaaacgc
    22201 ccctcttttt ttcatgtttt tctccatagc acaatgattc aggagaaagc atggtacaaa
    22261 ttgtcaggag cgcaagttgc ttcaggccgc gtgtgaggca tcttcctctt cggattcagc
    22321 accgacgacc tgtggggacg gtaatttgcc ggttttcaga atggtgctca gcacatcttt
    22381 ttgttctgcc agccatagcg aaagggcttc acgttgctcg tcttccattt gcactggaga
    22441 atttgtcagc caggtgtcaa gcagatccgc cgtatcaagc attttatcat aagcatcggc
    22501 ttcctttttg ctggtaaacg acattttctc ttcgccctca cgaatgacta cgtatttaac
    22561 ttcaaccgcc atttgcagcc tctcataata actgtgattt tatacagtat atttcttttc
    22621 ggttgagaaa tcaacatcag caataaagac acgcgcaaac gttttcgttt atactgcgcg
    22681 cggaattaat caggggatat tcgttatgac gttattaggc actgcgctgc gtccggcagc
    22741 aactcgcgtg atgttattag gctccggtga actgggtaaa gaagtggcaa tcgagtgtca
    22801 gcgtctcggc gtagaggtga ttgccgtcga tcgctatgcc gacgcaccag ccatgcatgt
    22861 cgcgcatcgc tcccatatca ttaatatgct tgatggtgat gcattacgcc gtgtggttga
    22921 actggaaaaa ccacattata tcgtgccgga gatcgaagct attgccaccg atatgctgat
    22981 ccaacttgaa gaggaaggac tgaatgttgt cccctgcgct cgcgcaacga aattaacgat
    23041 gaatcgcgag ggtatccgtc gcctggcggc agaagagctg cagctgccca cttccactta
    23101 tcgttttgcc gatagcgaaa gccttttccg cgaggcggtt gctgacattg gctatccctg
    23161 cattgtaaaa ccggtgatga gctcttccgg caaggggcag acgtttattc gttctgcaga
    23221 gcaacttgct caggcatgga agtacgctca gcaaggcggt cgcgccggag cgggccgcgt
    23281 aattgttgaa ggcgtcgtta agtttgactt cgaaattacc ctgctaaccg tcagcgcggt
    23341 ggatggcgtc catttctgtg caccagtagg tcatcgccag gaagatggcg actaccgtga
    23401 atcctggcaa ccacagcaaa tgagcccgct tgcccttgaa cgtgcgcagg agattgcccg
    23461 taaagtggtg ctggcactgg gcggttatgg gttgtttggt gtcgagctat ttgtctgtgg
    23521 tgatgaggtg attttcagtg aggtctcccc tcgtccacat gataccggga tggtgacgtt
    23581 aatttctcaa gatctctcag agtttgccct gcatgtacgt gccttcctcg gacttccggt
    23641 tggcgggatc cgtcagtatg gtcctgcagc ttctgccgtt attctgccac aactgaccag
    23701 tcagaatgtc acgtttgata atgtgcagaa tgccgtaggc gcagatttgc agattcgttt
    23761 atttggtaag ccggaaattg atggcagccg tcgtctgggg gtggcactgg ctactgcaga
    23821 gagtgttgtt gacgccattg aacgcgcgaa gcacgccgcc ggacaggtaa aagtacaggg
    23881 ttaaacccgg caaaaaaacg ctacaaaaat gcccgatcct cgatcgggca ttttgacttt
    23941 tacagcttag cgccttctac agcttcacgc gccagcttag taatgcggtc gtaatcgccc
    24001 gcttccagcg catctgccgg aaccagccag gaaccaccga tgcacagcac gcttttcagc
    24061 gccaggtagt cacggtagtt agccggagaa ataccacccg tcgggcagaa acggacctgg
    24121 gagaacggac ccgcgatcgc ctgcagggct ttcacgccgc cgttagcttc agccgggaag
    24181 aatttgaact ctttcaaacc gtagtccata cccagcatca gttcggaaac agtgctgatc
    24241 cccggaatca gaggaatagt cccttcggta gcagctttca gcagcggctc ggtcagaccc
    24301 gggctaattg cgaactgtgc acccgcttca gtgacttctg ccagctgctg tggattcagc
    24361 accgtaccgg cacccacaat cgcttcaggc acttctttgg cgatagcacg gatagcgtca
    24421 actgcacact cggtacgcag agtcacttcc agaacgcgca ccccaccagc aaccaacgct
    24481 tttgccatcg gcaccgcgtg ttccagtttt tttaccacga taaccggtac aaccgggccg
    24541 gtggtcagga ttgattctgc acttgttttc cagtttttca tcagagtttt ctctcgcctg
    24601 attacaaatt tgtcgtctta aaaagtgata caggttgcgc cctgttcggc accggacagt
    24661 ttttcacgca aggcgctgaa taattcacgt cctgttccca cgcgtgacgc gctcaggtca
    24721 ggaatgtgcg gttcgcgagc agccagttcc gcttcgtcta ccagcagcgt cagttcgcct
    24781 gtctgtccat tcacacgaat gatgtccccg tcgcgcactt ttgccagcag cccgccatcg
    24841 taggcttctg gtgttacgtg gatagctgac ggcactttac ctgaagcgcc ggagagtcgt
    24901 ccatcggtaa ctaacgcaat tttgaaacac cggtccaata atacaccaag tggcggcatg
    24961 agtttatgta attctggcat tccgttcgct tttggcccct gatgacggac aacaacgaca
    25021 caatcgcggt ccagcaaacc cgcttcaaag gccggcataa cgtcatgctg gctttcaaaa
    25081 acaaccgctg gcgcttcaat cacctggttc tcaaccggca cggcagaggt tttcataacc
    25141 gcacggccca ggttaccgct taacactttt gtcccaccat gatgagagaa aggttgttcg
    25201 aaggaagcga tcacattgct gtcgagtgat ttttccgccc cttcccgcca gtccagttca
    25261 ccattattca gccatggttc aagggtataa cgagacagac caaagcctgc caccgtattg
    25321 acatcttcat gcagcaggcc tgctttgagc agttcacgca ccagaaccgg tacgccacct
    25381 gccgcctgga agtggttaat atcggccgga ccgttcgggt agaggcgtgc catcagcggt
    25441 acaacatcag aaaggtcaga gaagtcatcc cagttaatct gaataccggc cgcgcgcgcc
    25501 atcgccacca ggtgcatggt gtggttagtg gaaccaccgg tcgccagcag tgcaacgata
    25561 ccgttcacca ccactttctc atcgatcatc ttaccgatcg gcatccattc attaccatta
    25621 ccggtcatgc gtgtaacctg acgcgcagcg gcagcggtca aagcatcgcg cagcggagaa
    25681 tccggatgaa caaaagaaga gcctggcaac tgcatcccca taaactccac caccatctgg
    25741 ttggtgttgg cagtaccgta gaaagtacat gttcccggcg catggtaaga cgcggcttct
    25801 gactccagta aggccatgcg gtccacttta ccttcggcat aaagctggcg aatacgcact
    25861 ttttctttat ttggcaaacc gcttgccatc ggtccagacg gcacaaacac cgcaggcaaa
    25921 tgaccaaacg acagggctgc catcgtcaga cccgggacaa tcttgtcgca cacaccgagg
    25981 aacagagcac catcaaacat gttatgggac agccccaccg ccgcagacat cgctatcact
    26041 tcgcggctta gcagcgacaa ttccattcca tcctgcccct gggtgacacc atcacacatc
    26101 gccggaacac cgcccgcaac ctgaccaacc gcattcgctt catgcagggc tttacgaatg
    26161 atttctggat agtgttcata aggctggtgc gcggagagca tgtcgttata ggaggtgatg
    26221 atggcgatat tgttacgcaa catgcttttc aaagaggctt tgtcttctgg ctggcaggca
    26281 gcgaaaccgt gtgccaggtt accgcatgcc aactgcgaac gatgaacggt cgacgttttc
    26341 gcttgttcta tccgggcgag ataagcagag cgagtctcgc gcgaacgttc aatgattcga
    26401 tttgttacgc gtaacaattg tggattcata aaggctcctg aaattgagtt gtcagagcag
    26461 gatgattcac aacgcgtttc attcagagga tttatgactg aaacgcctgt aaccggagct
    26521 catagggcaa aaacgtttca gtcagtgtaa taaaaaaagc ctcgtgggtg aatccgcacg
    26581 aggcctgaaa gtgtaaaaat tgttctacaa tctgcgcaag atcatgttac cggtaaaata
    26641 accataaagg ataagcgcag atattactca aactcattcc aggaacgacc atcacgggta
    26701 atcatcgcca ccgaggcaac gggtccccag gttccggcct gatacggttt cggcgcatca
    26761 ttgtccatcg cccacgcctc agtaatggag tctacccatt tccaggcttc ttccacttca
    26821 tcacgacgta caaacagtgc ctgaatacca cgcatggttt ccagcagcag acgttcatag
    26881 gcatccgcca gatgcgtctg attaaaggtt tctgaatagc tcagatccag cttggtgatt
    26941 tgcaggttat gtttgtggtc aaggccagga actttattca gtacctggat atccacgcct
    27001 tcatcaggtt gcagacggat agtcagttta ttctgcggca gatcctgcca cgactcttta
    27061 aacagattca gttcaggtgt tttgaaatag accacgactt cagaacattt ggtcggcaga
    27121 cgtttaccag tacgcaggta gaatggcaca ccggcccagc gccagttatc aatgtcgacg
    27181 cggatcgcca cgaaggtttc tgtattgctg ctcttgttcg cgccctcttc ttccagatat
    27241 cccggcactt ttttgccctg ggcgaagccc gcagtatatt gcccgcgtac ggttttttcg
    27301 cgtacgttgg agcggtcgat gcggcgcagg gacttcagta ctttcacttt ttcatcgcgg
    27361 atgctgtctg cgctcaggtc agatggcgga gacatcgcaa tcatgcagag aatttgtagc
    27421 aggtggttct gaatcatgtc acgcatctga ccggctttat caaaatagcc ccagcgccct
    27481 tcgatcccca cttcttctgc cacggtaatc tcaacatgat caatggtgcg attgtcccag
    27541 ttattcacaa acagggagtt agcaaaacgc agcgccaaca ggttcagcac cgtttcttta
    27601 ccaagatagt ggtcgatacg gtaaacctgg cactcctcga agtattcgcc aacctgatca
    27661 ttgatttcct gcgaggtcgc cagcgacgtc cccagcggtt tctccatgac tacgcgtgcc
    27721 ggtttagcat tcagttttgc ctcgccaagc cctttgcaaa ttgcgccaaa agtgctgggc
    27781 ggcatggcaa agtagttaat ggtgatacga tttttttgat ccagcatcgc gccgagaagg
    27841 ttgaatgcag cagtgtcatt gacatcgaga ttacaaaaat ccagacgtgc actcagggtg
    27901 tcccataaac cttcatcaat ggtttctttc atgaaagttt cgagcgcctc gcggacaact
    27961 ttggtatatg ccgctttatc ccagtcagca cgccctacgc cgataatccg ggtgtccggg
    28021 ttgagctgac cggctttttc cagttgatac agggaaggca gcaatttacg acgcgcaagg
    28081 tcgcctttcg cgccgaaaat gaccaggtca caggcctggg ctgtttgcgt taccgccatg
    28141 tcattctcct taagttaact aacccggtac ttaagccagg gtatacttgt aattttctta
    28201 cggtgcactg tactgctttt acgagcttgc gaaaactgta aacgcttatc cacccgtgcg
    28261 attacgggaa aagcgcgcaa agtgcggcaa aactgataaa aaaatcatcg ttttccattc
    28321 ggtaaaaatc tgacactgat catgttatga aaaaaaataa caactttttt atctgctttt
    28381 gtcattaaca cggcacacag gcgtaatatc tgacaaaact gcatttcgat ttctttcagt
    28441 gcggaaatcg tcattacccg tgagtctctt tacatcatga atatgctgga aaaaatccag
    28501 tctcagctgg aacatttgag caaatcagag cgcaaagttg ccgaggtcat tctggcttcg
    28561 cccgataacg cgatccattc gagtattgct gctatggcac tggaagccaa tgttagcgaa
    28621 ccgacggtga atcgtttctg tcgcagcatg gacacgcgcg gttttcctga ttttaaactt
    28681 catctggcac agagtctggc gaatggcact ccctatgtta atcgcaacgt caatgaagat
    28741 gacagcgttg aatcatacac agggaaaata tttgagtccg caatggcaac gctcgatcat
    28801 gtccgtcatt cactggataa atctgccatc aaccgcgccg tcgacttgct cactcaggca
    28861 aaaaaaatcg cctttttcgg attaggctct tcagccgccg ttgcccacga tgcgatgaat
    28921 aagttctttc gttttaatgt tccggtggtg tactccgatg atattgtgct gcaacgcatg
    28981 agttgtatga attgtagcga cggagacgtg gtggtgctga tttctcacac tggaagaaca
    29041 aaaaatctgg tcgagctggc gcagctggct cgcgaaaacg acgcaatggt gattgccctc
    29101 acctctgcgg gtaccccgct cgcccgggaa gcaacgctgg caattaccct cgacgtaccg
    29161 gaagatactg acatttatat gcccatggtt tctcgacttg cacagctgac cgtgatagat
    29221 gtgctggcga caggatttac tttgcgacgc ggtgcaaaat tcagagataa cttgaagcgg
    29281 gtcaaggaag cgctgaagga atcgcgtttt gataagcagt tacttaattt aagtgacgat
    29341 cgctaaaaac gactgtcact gtcctaatct tatacgacat ccgaatgaga ttaatttatc
    29401 gccatcgcgg cgttatttca ttcggatttc atgttcaagc aacacctggt tgtttcagtc
    29461 aacggagtat tacatgtcca gaaggcttcg cagaacaaaa atcgttacca cgttaggccc
    29521 agcaacagat cgcgataata atcttgaaaa agttatcgcg gcgggtgcca acgttgtacg
    29581 tatgaacttt tctcacggct cgcctgaaga tcacaaaatg cgcgcggata aagttcgtga
    29641 gattgccgca aaactggggc gtcatgtggc tattctgggt gacctccagg ggcccaaaat
    29701 ccgtgtatcc acctttaaag aaggcaaagt tttcctcaat attggggata aattcctgct
    29761 cgacgccaac ctgggtaaag gtgaaggcga caaagaaaaa gtcggtatcg actacaaagg
    29821 cctgcctgct gacgtcgtgc ctggtgacat cctgctgctg gacgatggtc gcgtccagtt
    29881 aaaagtactg gaagttcagg gcatgaaagt gttcaccgaa gtcaccgtcg gtggtcccct
    29941 ctccaacaat aaaggtatca acaaacttgg cggcggtttg tcggctgaag cgctgaccga
    30001 aaaagacaaa gcagacatta agactgcggc gttgattggc gtagattacc tggctgtctc
    30061 cttcccacgc tgtggcgaag atctgaacta tgcccgtcgc ctggcacgcg atgcaggatg
    30121 tgatgcgaaa attgttgcca aggttgaacg tgcggaagcc gtttgcagcc aggatgcaat
    30181 ggatgacatc atcctcgcct ctgacgtggt aatggttgca cgtggcgacc tcggtgtgga
    30241 aattggcgac ccggaactgg tcggcattca gaaagcgttg atccgtcgtg cgcgtcagct
    30301 aaaccgagcg gtaatcacgg cgacccagat gatggagtca atgattacta acccgatgcc
    30361 gacgcgtgca gaagtcatgg acgtagcaaa cgccgttctg gatggtactg acgctgtgat
    30421 gctgtctgca gaaactgccg ctgggcagta tccgtcagaa accgttgcag ccatggcgcg
    30481 cgtttgcctg ggtgcggaaa aaatcccgag catcaacgtt tctaaacacc gtctggacgt
    30541 tcagttcgac aatgtggaag aagctattgc catgtcagca atgtacgcag ctaaccacct
    30601 gaaaggcgtt acggcgatca tcaccatgac cgaatcgggt cgtaccgcgc tgatgacctc
    30661 ccgtatcagc tctggtctgc caattttcgc catgtcgcgc catgaacgta cgctgaacct
    30721 gactgctctc tatcgtggcg ttacgccggt gcactttgat agcgctaatg acggcgtagc
    30781 agctgccagc gaagcggtta atctgctgcg cgataaaggt tacttgatgt ctggtgacct
    30841 ggtgattgtc acccagggcg acgtgatgag taccgtgggt tctactaata ccacgcgtat
    30901 tttaacggta gagtaagtac gttgccggat gcggcgaaaa cgccacatcc ggcctacagt
    30961 tcaatgatag ttcaacagat ttcgaatatt ctgaagcaaa cttgaactta tcatcaggcg
    31021 aaggcctctc ctcgcgagag gcttttttat ttgatgggat aaagatcttt gcgcttatac
    31081 ggctggattt cgcccggttt gcgagttttc agcaatttta gtatccaggt gtattgttct
    31141 ggtcgcggac caacaaaaat ctcgacttct tcattcatcc gccgcgcaat cgtatgatca
    31201 tccgcctcta acagatcatc catcggtggg cgcacctgaa tcgtcagacg atgcgtcttg
    31261 ccatcataaa tcggaaacag cggtacaacg cgcgcacggc acactttcat caaacgacca
    31321 atcgcgggca acgtcgcttt ataggtggca aagaaatcca caaattcgct gtgctctggg
    31381 ccatgatcct gatcgggtaa ataatatccc cagtacccct gacgtaccga ctggatgaat
    31441 ggtttaatac cgtcatttct cgcatgcaga cgaccgccaa agcgacgacg caccgtgttc
    31501 cagacataat caaaaaccgg gttgccctga ttatggaaca tcgctgccat tttctgccct
    31561 tgcgaggcca tcagcatggc aggaatatcg acggcccaac cgtgcggcac cagaaagata
    31621 actttctcgt tattacgccg catctcttcg atgatctcca gcccttgcca gtcaacgcgc
    31681 ggctgaattt tctccggtcc gcgtattgcc aactcagcca tcattgccat cgcttgcggc
    31741 gcggtggcaa acatctcatc aacaatcgct tcgcgttcag cttcactacg ttctggaaag
    31801 cagagcgaca gattgattaa cgcacgacgg cgtgagcttt ttcccagtcg tccggcaaaa
    31861 cgtcccagcc gtgccagaat gggatcacgg aactttggcg gcgttaaagc gatacccgcc
    31921 atcgctgcta cacccagcca tgctccccag tagcgcgggt ggcgaaagga tttatcaaac
    31981 tcaggaatgt attcgctatt attttttttc gtttccatgc ttttccagtt tcggataagg
    32041 caaaaatcaa tctggtgata gtgtagcggc gcaacttgcc ccgcaccaaa taaaaaaagc
    32101 cggtactgac tgcgtaccgg ctgcgaatgg atgttaatta atcaaaccgt agctgcggca
    32161 caatctcttt ggcctgcgcc aggaattcgc gacgatcgga gccggtcagc ccttcagtac
    32221 gcggcagttt tgccgtcagc gggtttacgg cctgctggtt tatccatact tcatagtgca
    32281 gatgcggccc agttgaacgt ccggtattac cggaaagcgc gatacggtcg ccacgtttca
    32341 ccttctgtcc cggtttcacc agaatcttgc gcaagtgcat ataacgcgtg gtgtagctgc
    32401 gaccatgacg aatagccaca taataacctg ctgcgccact gcgtttggca accaccactt
    32461 caccgtcacc cactgaaagc actggcgtac cttgtggcat ggcgaaatca acacctctgt
    32521 gtggtgcaac gcgacctgtc accggattag ttcgacgcgg gttaaagtta gacgagatac
    32581 ggaactgttt cgccgtcggg aatcgcaaga atcctttcgc cagaccagta ccgttacgat
    32641 cgtagaattt gccatcttca gcgcggatag cgtaataatc tttaccttct gaacgcaaac
    32701 gtacgcccag cagctggctt tgctcacgtt taccatcaag catttctcga gacattaaca
    32761 ccgcaaattc atcgcctttt ttcagtttgc ggaaatccat ttgccactgc atggctttaa
    32821 tcactgcgct cacttcggcg ctggttaaac cggcgtttct ggcgctggca acaaagctcc
    32881 ccccgacggt acctttcagc agattgttaa cccactctcc ttgctgcatt tcgctggtca
    32941 ttttaaaacc gttagcggca gtacggtcat aggttcgggt ttcacgacga gacacttccc
    33001 aggtgaggcg ctgcagttcg ccgtccgcgg ttaatgtcca ggagagttgt tgaccgattt
    33061 tcaggttacg caattctttg tcggccgcag ccagttgggt gatatcaccc atatcaatac
    33121 catactgatt gagaatgctg cttagcgtat cgccagtgga aacaacatat tcatgcacgc
    33181 ccgcttcacc ggcgattttg tcatccagtt cgtcctgggg aatggcttca tcttcttgtg
    33241 cagcttgatc aatcggctca ctggcttcag gtaagagcga acgaatttcg ttctgttcca
    33301 gctcaatggt tttgacaatt ggtgtggcat cacggtgata aacataaggc cgccagacag
    33361 cgacggccag agtaagaacg gtgagcgacc ccaacataac gcggtgtggt cgcggtaaat
    33421 tattaaacgc cagggcgaca gagcgggcta tctgttgcac gtaatcactt cctcattaat
    33481 ctcctttcag gcagctcgca tactggttgg ctaattgact caggaattct gaatagcttg
    33541 ttttacccag tttgatattc gtccccaggg gatccaacgt tcccatacga acggatgtcc
    33601 ctcgtgcgac gctctcaacg accgctggcc tgaactgtgg ctcagcaaaa acgcaggttg
    33661 ctttttgctc aaccaactgt gttcttattt catgtaaacg ctgcgcgcca ggttgaatct
    33721 cagggttaac ggtaaaatga ccaagcggtg tcagtccaaa ctgtttttcg aaatagccgt
    33781 aagcatcgtg aaaaacgaaa taacctttcc ccttgagcgg cgcgagctcg ttaccaacct
    33841 gcgtttcggt tgaggctaat tgtgcctcaa aatccttcag gttggcgtca agtttggctc
    33901 gactttgcgg cataagttcc actaattttc catggattgc aaccgctgta gcccgcgcta
    33961 tctctgggga aagccaaaga tgcatgttga aatcgccgtg atggtgatct tcgtcacttt
    34021 tttccgcgtg gtcgtgatca tcatcatcgc cgtgaatact tttcatcagt agcggtttca
    34081 catcttcaag ctgcgcaatc gttacctgct tcgctcctgg taatttgctt accggttttt
    34141 gcataaacgc ttccatctcc gggccaaccc aaacgactaa gtccgcgttc tgtaagcgtt
    34201 ttacatccga tggacgcagt gaataatcat gttctgaagc gccgtcaggc agtaaaacct
    34261 ccgtttctgt tacaccatca gcaatggcag aagcgatgaa cccaacgggt ttaagcgaag
    34321 cgacaacggc ggcatctgcg gcctgtgttg cacctcccca gagagcggcg gataatgctg
    34381 cgaaaagaag cgttttttta tgtaacataa tgcgaccaat catcgtaatg aatatgagaa
    34441 gtgtgatatt ataacatttc atgactactg caagactaaa attaacatga caagtctggt
    34501 ttccctggaa aatgtctcgg tttcttttgg ccaacgccgc gtcctctctg atgtgtcgct
    34561 ggaacttaaa cctggaaaaa ttttgacttt acttgggcca aatggcgcag gtaagtcgac
    34621 actggtacgg gtagtgctcg ggctggtaac acccgatgaa ggggttatca agcgcaacgg
    34681 aaaactgcgc atcggctatg taccgcagaa gctgtatctc gacaccacgt tgccactgac
    34741 cgtaaaccgt tttttacgct tacgccctgg tacacataaa gaagatattt tgcctgcact
    34801 gaaacgtgtc caggccgggc acctgattaa cgcaccgatg caaaagctct ctggtggcga
    34861 aacgcagcgt gtactattag cgcgagcatt gttaaatcgc ccgcaattat tagtgctgga
    34921 tgaacccact caaggcgtgg atgtaaatgg ccaggtggcg ttatatgacc ttattgacca
    34981 actgcgtcgt gaactggatt gtggcgtttt aatggtttct cacgatctgc atctggtgat
    35041 ggcaaaaacc gatgaagtgc tgtgcctgaa tcaccacatt tgttgttccg gcacaccgga
    35101 agttgtttcc ctgcatccgg agtttatttc aatgtttggt cctcgtggtg ctgaacaact
    35161 gggtatctat cgccatcatc ataaccatcg tcacgattta cagggacgaa ttgttttgcg
    35221 tcggggaaat gatcgctcat gattgaatta ttatttcccg gttggttagc cgggatcatg
    35281 ctcgcctgtg ccgcgggtcc gctgggttcg tttgtagtct ggcgtcgtat gtcttatttc
    35341 ggtgatacgc tggctcatgc ctcattactt ggcgtcgcgt ttggtttgtt gctggacgtg
    35401 aatccattct atgcggtgat tgccgttacg ctgctgctgg cgggcggtct ggtatggctg
    35461 gagaagcgtc cacagctggc gatcgacacg ttattaggga ttatggcgca cagtgccctg
    35521 tcgctgggcc tggtggtcgt tagtctgatg tctaatattc gtgttgattt gatggcttac
    35581 ttgttcggtg atttgctggc agtgacgccg gaagatctca tctccatcgc cattggcgtg
    35641 gtcatcgtgg tggctatttt gttctggcaa tggcgcaatt tgctgtcgat gacgattagc
    35701 ccggatctgg cgtttgttga tggtgtgaaa ttacagcgcg tgaaattgtt attgatgctg
    35761 gtgacggcat tgacgattgg tgtagcgatg aaatttgtcg gtgcgttgat tattacttcg
    35821 ttgctgatta ttcctgcagc tactgcgcgt cgctttgccc gcacgccgga acagatggct
    35881 ggtgtcgctg ttttggtggg gatggtggca gtgactggcg gtttaacctt ttccgcattt
    35941 tacgatacgc cggcgggtcc gtcggtagtg ctatgtgcgg cactgttatt tattctcagt
    36001 atgatgaaaa agcaggccag ttaatctgtc gctgaacaca tttgtcggat gcggcgcgag
    36061 cgccttatcc gacctacggt tcggtatctc tggtagtcct ggtaagacgc gaacagcgtc
    36121 gcatcaggca tattgccagt gccggatgcg gcgcgagcga ccaatccgac ttacggcatt
    36181 tctggcggcg ttatgccaaa gtgattccac gcccgcgtcg tcgccatacg cccacgcggt
    36241 gtacgctgca aaaagccttg ctgaatcaaa taaggttcca gcacatcctc aatggtttca
    36301 cgttcttcgc caatggctgc cgccaggtta tccagaccta caggtccacc aaagaactta
    36361 tcgattaccg ccagcaacaa tttgcggtcc atataatcga aaccttcagc atcgacattc
    36421 aacatatcca acgcctgagc agcgatatct gccgagatgg tgccatcgtg cttcacttcg
    36481 gcgaaatcac gcactcgacg cagcagacgg ttggcaatgc gcggcgtacc gcgagcgcga
    36541 cgagcaactt ccagcgcgcc gtcatcactc atctcaagcc ccataaagcg tgcgctgcga
    36601 ctgacgatat attgcagatc cggcacctga taaaactcca gacgttgcac aataccaaaa
    36661 cggtcgcgca acggtgatgt cagcgaacct gcgcgcgtgg ttgcaccaat cagggtaaac
    36721 ggcggcaaat caattttaat ggagcgtgcc gccggacctt caccaatcat gatatccagt
    36781 tggtagtctt ccattgccgg gtacagcact tcttcaacaa ctggcgataa acggtggatc
    36841 tcatcaataa acagcacgtc atgcggttca aggttagtga gcatcgcagc caaatcgccc
    36901 gccttttcca gcaccggacc agaagtcgtg cgtaaattaa cgcccatttc attggcgaca
    36961 atgttggcaa gcgtagtttt acccaacccc ggaggaccaa aaatcaacaa atgatcgagg
    37021 gcatcgccgc gcagtttcgc tgctttgatg aaaatctcca tctgtgaacg aacctgcggc
    37081 tgaccaacat actcttccag taatttaggg cgaatggcgc gatctgctac atcttccggc
    37141 aaagtggtac cggcagaaat cagacggtct gcttcaatca tcctttacct cataacgcgg
    37201 cgcgtagggc ttcgcgaatt aatgtttcac tgctggcgtc agggcgagcg attttgctca
    37261 ccatgcggct ggcttcttgt ggtttatagc ccagcgccac cagcgcagca accgcttcct
    37321 gttcagcatc gttggttgcc gggctggcag gcgacgtgag caccagatcg gcggctggcg
    37381 taaagagatc gccatgcaga cctttaaatc ggtctttcat ttcaacaatc aagcgttcgg
    37441 cggttttttt gccaataccc ggcagtttca ccagcgcccc cacttcttca cgctcaacgg
    37501 cattaacgaa ctgctgcgct gacattccgg agaggatcgc cagcgccaac ttcgggccga
    37561 cgccgttggt tttgatcaac tctttgaaca acgtgcgctc ttgtttattg ttaaaaccgt
    37621 acagcagctg cgcgtcttca cgcaccacaa agtgggtgaa aacgatcgct tcctgtcccg
    37681 cttcagggag ttcataaaaa caggtcatcg gcatatgcac ttcatagcct acgccgccca
    37741 cttcaattaa caccagcggg ggttgttttt caatgatgat gcctctgagt ctgcctatca
    37801 catgacgctc ctgcgtaatg aatcaaagat aatgctgtat gataaaaaaa tgctggatag
    37861 atatccagcg aaggatgaag aaaacttgcg aggtgtctcg atgatctgaa aaatg
//
LOCUS       DAPGWD010000031        36340 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPGWD010000031 DAPGWD010000000
VERSION     DAPGWD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.031"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            67..783
                     /gene="nanC"
                     /locus_tag="RHX88_001508"
     CDS             67..783
                     /gene="nanC"
                     /locus_tag="RHX88_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDV2230209.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            803..1909
                     /gene="nanM"
                     /locus_tag="RHX88_001509"
     CDS             803..1909
                     /gene="nanM"
                     /locus_tag="RHX88_001509"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDV2230210.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNNTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1974..2954
                     /locus_tag="RHX88_001510"
     CDS             1974..2954
                     /locus_tag="RHX88_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDV2230211.1"
                     /translation="MDAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPSIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(2962..3084)
                     /locus_tag="RHX88_001511"
     CDS             complement(2962..3084)
                     /locus_tag="RHX88_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV2230212.1"
                     /translation="MISFVFLLPVYPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            3166..4038
                     /locus_tag="RHX88_001512"
     CDS             3166..4038
                     /locus_tag="RHX88_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV2230213.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSHYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            4208..6283
                     /locus_tag="RHX88_001513"
     CDS             4208..6283
                     /locus_tag="RHX88_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2230214.1"
                     /translation="MTLVDSVEAGRLTISELIDALAKDKNYTASRWYQRYRAFTTLLQ
                     QTSTFAEPATDGLVKQLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
                     FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKVA
                     STTSITRSEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWFLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPGNVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSEAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKDFDQEIWNMPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            6276..7619
                     /locus_tag="RHX88_001514"
     CDS             6276..7619
                     /locus_tag="RHX88_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDV2230215.1"
                     /translation="MGEVISVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIQTQQATLQAQRMPLLEIFINQFLHSVSQLLKQGLRSDYVSKQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPYELPSHLKVLPQAATYSLVKHGVKDCFKLRPDLLIQSNKPVQTE
                     MVMDTKWKLVNSSQQTKSLYGLAQADFYQMFAYGQKYLGGNGEMYLIYPAHDDFSQPI
                     PQHFAFSETLKLWVVPYRIMAKRGERMMWPNDVSCTMSPKLDRMAASVSYSGGMR"
     gene            7616..8002
                     /locus_tag="RHX88_001515"
     CDS             7616..8002
                     /locus_tag="RHX88_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230216.1"
                     /translation="MTHEYQRQSLEQLITQQTATPRSAAVVFNENQTLWQTLGFSQSQ
                     VSLWLASLPQSNHLKGPTTATYQVIPDIAAHLVSLLHQAGGRMPLAQVLKKLPAGITT
                     SEQQIRKLAQKHAQIEIKGPLLVLIN"
     gene            8053..10200
                     /locus_tag="RHX88_001516"
     CDS             8053..10200
                     /locus_tag="RHX88_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDV2230217.1"
                     /translation="MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAG
                     LIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDLAYQLLSDIEGDWPVYAVVDDGL
                     NHRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFH
                     ENVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFNWQY
                     VESHGDKAVAQIKAAFEEFKSHENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEA
                     IQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESKGGLGVYLTPNPVKQ
                     AMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADD
                     KKESLKQKLRDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSF
                     DLICTNPPFGTPKFDKGKNGQNSKANYEANMEQVLGGFRPTKKVVDSYNAWYDHVKMK
                     WEDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPDSKGNWQ
                     PVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEF
                     VGGKAIVKAVISLPSDCFKLSGTGAKTSILYLQKRHANPNQPEQFLPEPQTDVFMAVA
                     ETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE"
     gene            10200..11675
                     /locus_tag="RHX88_001517"
     CDS             10200..11675
                     /locus_tag="RHX88_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV2230218.1"
                     /translation="MATIFKRTPEQLSERRIDPWHYQPRFERQINKIKDNLGALPLEA
                     VIDSKRGVASGATPLGANYLEKGRVKFYRTSEVEEMFLQAEEAVFINDEDDEKLKRSR
                     LVDGDVLLTITGAKFGKSAVVSAKHLPANISQHSVRFKPDPKKVDAYFLVAYLNSKTG
                     QVAIWKEAYGATRPAIDFPSVRSLAVPKVLPLAQKYIGDKVRQAEQLRAWAKRLEGMA
                     DRKIKDLFHFNLVDSLTLKPRRMKQQVLSAVSLAPEFARAADSQMTFRNSSKLSNFIS
                     KCKCGDPIKSEDRVPGPYFYYGASGPIDTHTEFNFNGKYLIIAQDGSIGCANVADGKF
                     WANNHVWVLKVKDEYDIESICRFLDKHFPCWKGVTTGSVVPKVTSENLLNILIPIDIA
                     KNREIGSKLRLAVTTAAYAKKLTASAKTLVESLIEGQLTEQQLIQAQQALEDGDSSFD
                     QAILSKLSAEGYAIEGATPLFSDVDELYSLLEEAAQAEAEE"
     gene            11678..12979
                     /locus_tag="RHX88_001518"
     CDS             11678..12979
                     /locus_tag="RHX88_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230219.1"
                     /translation="METRGTDSSTTRQYNDPVNSEAALNLCLQLWQQGGLNANKAALL
                     LAAVPALRSLLQPIIQPKKNDAETDIVSAFSLTAPLLDAFNDLSQSGEWQLALLGLNP
                     DVRQHWINLAAARCQEAGTMSDTMVLVKLIQQLGNASEWVLAQLESTATTPQIIAGPL
                     AQTERDLLGHSLNDNAAIPALCRILRTSYTLFTVSEQNEPPAPIQAVDVTAKQLTNNW
                     CSGRLLALPNTLLDEHNLKPNADWLLVSRSGHDNMPLTELFAQQPWLFLLSLIIFVQD
                     AWAAEQRGGLLLTLPAGQNAFAPGQINVAVQGIEGDEVSLGSLAEFLVLLLGELNIPL
                     YPALDANTESINRLNRVLSSFIAELLAKKIWQFTEAGRGEAGQYRIHTSFSDACYSLP
                     LAPLFGYKSQTLQRTIKQLAQNCYANKKRAANRINLQGSSL"
     gene            12976..17262
                     /locus_tag="RHX88_001519"
     CDS             12976..17262
                     /locus_tag="RHX88_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230220.1"
                     /translation="MSFELNEQMVDSLAKQHRWAATGVSDYPPRDPLVGQSRFFKRFQ
                     TFLHTVDHDDDRFAHVFAVEAEWGRGKSRLGHELVAQINDCSRGWFVRDEQGQLHDKQ
                     LFNRDTQDKYLALYIRYSQVASDYQNSDNWFAFGLYQALLPLATQTFDGSIQSEIAKQ
                     AYERLMPFGFKSELLADALQLNANHSEEDLYTDQTLVVSLVQAAYKVLKSFGVQYMLV
                     VLDELETVAEAATFGLEDEQNKRLDGQAIRLIGKAIKEEDPRRKLPWLRYVALCSPLL
                     GQQLREIQSVARRFELVELEHNAFADVSDYVASLLKDGKLGFNYPAGLVEAAYAMSGA
                     NFGWFNVVMANVDAVLSQYQQAGRSIKDVGDLFEAVLAGSGRVARHVLDKGAIEGIQT
                     RDQALLTECRSLLYGQLPVRLAEMGYATELLALKNEDLEPIAAHYRKLSIDRLQCRQA
                     LEQAKFRRDKDEWYYPAVDQALNLDTLLDNLRTFSIKEQAASGDKGAVLLPLSKGEFK
                     YLLSLLYDHPAIEFAADALWHKLVGETTELDASEATHIGPSVAMLLRLDLRYRSAQQN
                     SLIFKDPAMGDAHEQAMSELSKQSSQKPLIKALARVTGFVRLLDNNWTYDENLLSNKA
                     GSDDAPLAILTEPRGRQGGLLTLDGFKLHPEGKALFAWVSNLIELNNLHTFAANHSHQ
                     NGRTPVLALTASAHLMEQYSRLDERNELRDAILLHYVNPSEADQLERIGLSLAACQLH
                     GVNLTADSFTAKFKNKLHALTTFATDAIHKWRQRLQQRGLIAWPLKVDGKLSPNDRDL
                     LFKGWYQLAIAHPELNGILDLQQQHGVPVNELSSLLDKLKVPGSYIAKGYTADEHAGL
                     FTELNNVQRSQAQIPLFLARIAHPNKKHKWQFEGFKQQFYFAYVAETSVTAKGVFNDW
                     MWWCGELNLLTLTNPTEKQAIWEHYPRSRLENAIREAQNWFRGNDMGSYAANVEVMSR
                     VYGYARINEMFAPLGKNKLGFVTVEAKEQLEKAQSLFNVLKQQEEQLADMVEANDTKV
                     LAGLIHKRAEVLELVAKVKPLNSSRPMLKDAHILSLEDKTTSLYQRIEQACLFAEFVE
                     CSAERINNRLVDLIIDVETECAPLTNFPKRLYTNTLRTIGHILDGALKDDTSSATGRK
                     EQQADSDTLLHYLRKLDLGRAHDKLSALAQEVGLNLHNDQQLPIAEIQGHILSSYRNC
                     KERFSKLVNNLTEQKLRAQQLQEWLSSATAEYQFADDIAELPKLVMKLQLIEDAIADL
                     PSDAESKRQSMQNSLRNGQFSSLRDLPEELLKPARGQLTPIQGQLLKIEERLNQVRRN
                     SIAQVNSWLPLLKPLLASQKQAEPAALTLEDVSNLGVRELQQVCESTQAKWQSQGEQI
                     LNDTGLTLADWQPIYKALSQNQEPVLTPEQQKGLVDKGIVKMRLTFATGL"
     gene            17273..18121
                     /locus_tag="RHX88_001520"
     CDS             17273..18121
                     /locus_tag="RHX88_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230221.1"
                     /translation="MSRYLSAALASNRKGRFLQTVAGATPLMKDWISSPPASGLLIVQ
                     AEELTDANTMQHLYHWAMQAGCAALVINLKAEQFTLLAQLPYPLDWQLVPASLRGQEP
                     GLTALLASETDQAIAGFTGSADRYQHQAGDVVHTRYIRKHSNSGLLAFTTLPLWSLTL
                     LDHSELLVSWLNWFVDHAGIAERIIEPKAPSTDYTPDKHDLVVLLLLYAGGGMNLQAL
                     SEHNAVKLMFDVNSLDIVKRGEMLRQHDFIDDAGITATGKTCLQASQYWAYAPLLGEQ
                     LHTGTL"
     gene            18124..19281
                     /locus_tag="RHX88_001521"
     CDS             18124..19281
                     /locus_tag="RHX88_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230222.1"
                     /translation="MNENKTMNLAQSLAIDSQLRLPGSAGRLLKQVRDIEQVAPLFRD
                     AGLVKAVPKPTIVNLRVAGIATKSCLDKVLGGFIYASAAVRTDLLIDDNKVSTVGSDS
                     VSELDDIDFEAQRKRLDLIEIRQAYQLVEKKLLGYEPGDLILLDTPLFLARDMAPLER
                     NVRHKQEYEKTRQVIETFWQNHRSRIFPWNQNGVVLASILAERFSAIVSIAKQDLRTE
                     QGRKQILASDGFSKELMQNLEGLDESLVGVGDLRFINGILGNYSRTIAFRLSEQEKRI
                     EPNIEAKQGLIGFHFRSARGGQIKMVQLAGDEPEWKSADLDLVASRLMVLDMQNKGNA
                     MPLPQLLGYQQLGILPKFATFYRQGLHQALKNNDIEAGWLAGLDEGLNNGL"
     gene            19271..20956
                     /locus_tag="RHX88_001522"
     CDS             19271..20956
                     /locus_tag="RHX88_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV2230223.1"
                     /translation="MAYKVLGKLVGNTGDASQLTMVVQDSFSVRRGEFVRIMHQERKD
                     EGQVAVLGRVTKISRTNMLYNAGFGDGVTELELLPGANVTGENMFAQVELVGYRDPVT
                     RQIKIPRRPLNPGTAVETVDFQFLSDFYEFNEHSSLHLGNLVGYDKGENTVPVFIDVN
                     KLVTEHLAVLAMTGSGKSYTVGRIIERLVAVNNGTVVVFDPHGEYGKALAGGNLQFSS
                     FLEATDDKRDQEALPLIKQTFEKLQAAGAGIQVYSPQHESFKHKYASKNTPLALQFDH
                     FEMDDIAEILPGLTEPQQRVLDVAIRYWRSADKTEPRDINRLRFLLGDGIEELKEWDD
                     LSEAEAKALSGRSAAVASMKLSRVLNEAKSFYSATMAEPTDIYKMVGRPSNQQGRLVV
                     VDLQGLSDTAKQVICALLSSEILKAASSKTDPLRPCFIIYEEGHNFAPAGGNAVSHRI
                     IKKIAGEGRKFGVGFGIVSQRPSKLDSDVTSQCNTLITMRLKNPDDQRFIAKASDMVS
                     KADLEELPSLSTGEALVCGRSIPAPLLVKVGSKALIHGGESPEVLRVWGSFSG"
     gene            21093..21506
                     /locus_tag="RHX88_001523"
     CDS             21093..21506
                     /locus_tag="RHX88_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230224.1"
                     /translation="MAVVECPAPGTFGADIRSDSGWFHKSSASPVCLIEFERFDGSAK
                     GQQKLEEKLKNLLEAAQRWNHCPKTLVLSAWSQGLVGVPDTQKLKDICRMGFTSSTGT
                     QVIAAPDVEVVFSRFLFIKNLNMIVLDRIHYEVLM"
     gene            21503..22972
                     /locus_tag="RHX88_001524"
     CDS             21503..22972
                     /locus_tag="RHX88_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDV2230225.1"
                     /translation="MNIKSAHFLPGTNKRLGDKYLLLECLGDGSHGWVWRAERLKDGK
                     IVAVKIPKDITREDRQLAEGKELLNVEPHENIVQIFDMGRIDNEWFYIEMEYFPSQTL
                     AQKLDDRQRNFGQTYERLFRIYRQVLCAVHYLAELPVPISHGDIKPHNILVGERDLVK
                     LTDFGSSALPEEIYVRTRENGGTVLYSAPEFSNVDSRRGSLEELLLGDIYSLGVLLYQ
                     LLTGKLPHDTPAQVQRHAPFKLPTEINSSIHTDLEQVVLTCLQKRAEDRFSTISDLIH
                     AFDSAATKQLKVGAIAPVFQTKPAQDWSSAVLEAMSDQSYQKAAQLASQEYSRSKDLQ
                     ALHQQLIALYRANRLFDFEKVVEDNKAILLEGKLTQPAALFELIVKANLQLRNISQAN
                     FWLLQRKQAEAENAATMYLESSILGLEAKYSEARVLLEKVNQTTPMKFHVLSQLILVC
                     EQMRDYSGAAAYLKAALRVAPLDNSMKEKKELYAKLGVI"
     gene            complement(23174..24439)
                     /locus_tag="RHX88_001525"
     CDS             complement(23174..24439)
                     /locus_tag="RHX88_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDV2230226.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLVDARGRRDEARKLLANGVDPSENKKAVKVEHEQEVITFEVV
                     AREWHASNQKWSASHSARVLKSLEDNLFAAIGKRNIADLKTRDLLVPIKAVESSGRLE
                     VAARLQQHTTAIIRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLQRIY
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFDTAMWTIPGEREPLEGVKHSQRG
                     SKMRTPHLVPLSRQALTILEKIKSMSGNRELIFVGDHDPRKPMSENTLNKALRVMGYD
                     TKTEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERMLM
                     LQWWADYLDANRMAAITPFDYAKINSSFR"
     gene            complement(24641..24725)
                     /locus_tag="RHX88_001526"
     tRNA            complement(24641..24725)
                     /locus_tag="RHX88_001526"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24689..24691),aa:Leu,seq:caa)
     gene            24921..25940
                     /gene="ahr"
                     /locus_tag="RHX88_001527"
     CDS             24921..25940
                     /gene="ahr"
                     /locus_tag="RHX88_001527"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDV2230227.1"
                     /translation="MSMIKCYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            26070..27572
                     /locus_tag="RHX88_001528"
     CDS             26070..27572
                     /locus_tag="RHX88_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDV2230228.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANSVFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGVLGSLLGGRRR"
     gene            complement(27691..28773)
                     /gene="lptG"
                     /locus_tag="RHX88_001529"
     CDS             complement(27691..28773)
                     /gene="lptG"
                     /locus_tag="RHX88_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV2230229.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(28773..29873)
                     /gene="lptF"
                     /locus_tag="RHX88_001530"
     CDS             complement(28773..29873)
                     /gene="lptF"
                     /locus_tag="RHX88_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV2230230.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            30140..31651
                     /gene="pepA"
                     /locus_tag="RHX88_001531"
     CDS             30140..31651
                     /gene="pepA"
                     /locus_tag="RHX88_001531"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDV2230231.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            31785..32228
                     /gene="holC"
                     /locus_tag="RHX88_001532"
     CDS             31785..32228
                     /gene="holC"
                     /locus_tag="RHX88_001532"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDV2230232.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            32228..35083
                     /gene="valS"
                     /locus_tag="RHX88_001533"
     CDS             32228..35083
                     /gene="valS"
                     /locus_tag="RHX88_001533"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDV2230233.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(35139..36335)
                     /locus_tag="RHX88_001534"
     CDS             complement(35139..36335)
                     /locus_tag="RHX88_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDV2230234.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
BASE COUNT         9975 a         8528 c         9014 g         8823 t
ORIGIN      
        1 acataaatgc taattgttta tgcgggcttt gtattgcttt ctgtatccta caaatgagtg
       61 aaatttatga aaaaggctaa aatactttct ggcgtattat tactgtgctt ttcgtcacca
      121 ttaatttctc aagctgcgac actggacgta cgtggtggat atcgtagtgg aagccacgcc
      181 tatgagactc gactcaaagt cagtgaggga tggcaaaatg gatggtgggc aagcatggaa
      241 agtaatacct ggaataccat tcatgataat aaaaaggaaa atgccgcact caatgatgtt
      301 caggttgaag ttaattacgc aattaaactt gatgatcaat ggacggtgcg cccgggaatg
      361 ttaacgcatt ttagcagcaa cggcacacgc tacggaccct acgtaaaact gtcctgggac
      421 gcgacaaaag atcttaattt tggcattcgc tatcgttacg actggaaagc ttaccgacaa
      481 caagacttat ccggtgatat gtctcgtgat aacgttcatc gttgggatgg atatgtcact
      541 taccatatta atagtgattt caccttcgca tggcaaacga cgctatacag caaacagaac
      601 gattatcgct atgcaaacca taagaaatgg gcgacggaaa atgcatttgt tctacaatac
      661 catatgacgc ccgatattac gccatacata gaatatgact accttgaccg tcagggtgtt
      721 tacaacggca gagataattt atcggaaaac agttatcgca ttggtgtgtc atttaaactg
      781 tagtagacag gagacagtca caatgaataa aacaataacg gcgcttgcta tcatgatggc
      841 ttcatttgcc gcaaacgcgt ctgtattacc ggaaactcct gtgccattta aaagtggtac
      901 cggagcaatt gataacaaca ctgtctacat tggtttaggt agcgcaggta cggcatggta
      961 caagctggat acacaggcca aagataaaaa atggacagcg ttagctgcat tccctggcgg
     1021 accaagagat caagcaacct ctgcatttat tgatggcaat ctgtatgtgt ttggcggcat
     1081 tggcaaaaac agcgagggct tgactcaggt atttaatgac gtacacaaat acaaccccaa
     1141 aaccaatagt tgggttaaat tgatgtcgca cgcgccgatg ggcatggcgg gccatgtgac
     1201 ttttgtacac aacggcaagg cttatgttac tggtggtgtt aaccagaata tcttcaatgg
     1261 ctattttgaa gatctcaacg aggctggaaa agattcaacc gctatagata aaatcaacgc
     1321 tcactatttt gacaaaaaag cagaagatta tttcttcaat aagtttctgt tgtcttttga
     1381 tccctcaaca cagcaatgga gttacgctgg cgaatctccc tggtacggaa cggctggtgc
     1441 ggcggttgtg aataaaggtg ataaaacctg gcttattaat ggcgaagcca aaccaggatt
     1501 gcgaacggat gccgtatttg aacttgattt caccggtaat aatttaaaat ggaataggct
     1561 tgctcccgtc tcatcaccag atggcgtcgc tggcggtttt gcggggataa gcaatgattc
     1621 tcttatattt gccggagggg ccggattcaa aggttcacga gaaaattacc agaacggtaa
     1681 gaactatgcg catgaaggcc tgaaaaaatc atatagcgct gatattcatc tttggcataa
     1741 cgggaaatgg gataaatcgg gtgaattatc gcaaggtcgg gcctacggag tatcattgcc
     1801 ctggaataat agtctattga ttattggcgg tgaaactgca ggcggcaaag cggtgacgga
     1861 ttcagttttg atctctgtga aggataataa agttacagta caaaactaac gcttcagggc
     1921 cccagtaaag gggccctgct atcaatttac catttcaatt acgcgaaaat tatatggacg
     1981 caataatatc gccagattat tactatgttc ttaccgttgc tggtcagtct aatgccatgg
     2041 cgtatggcga aggactgcca ttaccggaca gggaagatgc gcctcatccc agaattaaac
     2101 aattagcgag atttgcgcat acgcatcccg gaggcccgtc atgtcacttt aacgacatta
     2161 ttccactgac tcactgccca cacgatgttc aggatatgca gggttatcac catcctctgg
     2221 caacgaatca tcaaacacag tacggcaccg ttggccaggc actgcatatt gcacggaaat
     2281 tactgccctc tattcctgat aatgcagggg ttctcatcgt tccatgttgt cgtggcggct
     2341 cggcttttat cgcgggcagc gaagggacat attcagaacg gcacggagcc agccatgatg
     2401 cttgtcgttg gggatcggat actccgctat accaggattt agtcagcaga acgcgagccg
     2461 cactggcaaa aaatccgcag aacaaattcc tcggcgtatg ctggatgcaa ggcgaatttg
     2521 acttaatgac cagtgactac gcgtcacacc ctcaacactt taatcatatg gttgaagcct
     2581 ttcgtaggga tctaaaacaa taccattctc agcttaataa tattactgac gcgccgtggt
     2641 tttgcggcga taccacctgg tactggaaag aaaatttccc gcattcgtat gaagctattt
     2701 atggcaatta tcaaaataat gttttagcca atattatttt cgtcgacttc cagcaacaag
     2761 gtgaaagagg actgacgaac gcgcctgatg aagacccgga cgatttaagc acgggatatt
     2821 acggttcagc gtaccggtca ccggagaact ggacgacggc actgcgaagc agtcatttca
     2881 gcacggcagc ccgtcggggg gttatttctg acaggtttgt agaagcaatt ttgcagtttt
     2941 ggcgcgaaaa gtgagcgttc attaatttga tttattcgca tccatcaacg cttttaactc
     3001 ctccggcgta aaaggtggtt tgcgtcggtg caacatcgca tggcaattag ggtacacagg
     3061 cagcaaaaaa acaaatgaga tcaaagtaat atatcttcac ttgcaggctt gaacagccga
     3121 aaatttgcga gcatgatcgc aattatttca ctgccaggga tatcaatggc ttccagtaaa
     3181 tcgctacagc aggcaattgc caacataaaa atctggcaca aaggtgaaca gcgcgcgccg
     3241 cataagccat tgttattgct atacgtatta gcgggatacc tgaatggaca tccacgcctt
     3301 ttcgattatg gatcggaaat ctacgaacct ctgcacagtt tactggaacg ttttgggcca
     3361 cagcgttcac attaccgacc tgatatgccg ttctggcgat tacaaggcga cggattctgg
     3421 caattacaca atgccgagct ttgctcaaca gcagggagca gccgacaacc gccagtgaag
     3481 gaactgaacg aataccacgt tgcaggcggt ttcgatgaac agcactacgc cctggtaacc
     3541 ggcaataaaa aactcatcaa tactctggcg caacagatac tcgaagcgca cttcaccgag
     3601 agtattcagg aagaaattgc tgacgagctg ggttttgatc tccagcaaat ccgtaaacag
     3661 cgcgaccctt tatttcgcaa aaatgtatta cgcgcttata actaccagtg cgcgatatgt
     3721 ggctttaata tgcgccacga cgacaccacc gtagcgttgg aagcggcgca tataaaatgg
     3781 aaacagcaag gaggtccttg tgaaattcct aatggcctgg cgctttgcgc aattcaccat
     3841 aaagcctttg ataaaggatc gattggcctg gatgaaaata tgcgggtact ggtatcagat
     3901 gcggtaaacg gcggagggat cgttgagaga ttattctggg attttgacgg gaaaactatt
     3961 gcgttgccgc aggtgcgtaa aaattaccct tatgaggtgt ttgtagagtg gcatcggaaa
     4021 gaggttttta gggggtgagg ttgataacag cattcaccct acaaccttac attgtattta
     4081 aattagagct gagttaacag atagttgtat atataacagg cacttgtcac aacgattaac
     4141 tcaatatagt tagaatacct catattaatt aaaaaataag taatgtcaca acagaggaaa
     4201 agtcacgatg actcttgtag atagtgtcga agcaggtagg ctaacgatta gtgaattgat
     4261 tgatgccctg gcgaaagata aaaattacac agcttccaga tggtatcagc gataccgtgc
     4321 atttacgact ttgctacagc aaacctcaac ttttgctgag cctgcaacag atggtctggt
     4381 caaacagctt tggtatgagc gtgacaacgg cattgcaagt attcgccagg gcgttccatc
     4441 cttagcagaa tatcagcaaa gcctgccact gcttagagaa ctaactgaac gaattcggca
     4501 acagccggat gaagaaactt accaatatgt tggcaatgca cttcaacaag ctaaagaaaa
     4561 cggacttctc aagcgtatgt attggagttt gagaaatcgc gtctttgccg cgttctcgcc
     4621 agaaaactac accagtactg tggatgagaa tgcttttaat aaagcagcag aattcttaaa
     4681 tcagcacttc catctcggtt tggtactgac cggaaattgg ttacagaaaa actatgaatt
     4741 gaaacaagcc atacacgccc aatctcctga tacagatcct tattatgtga atatggccat
     4801 ctggcatctc tatgaattgc tccgtgaacg cgataatgaa caaaagcagg agaaagtagc
     4861 tagcactaca tccataaccc gcagtgagcc catcgagaac aagatcatcc tacattcacc
     4921 aactaacgtg atcttctttg gcccccctgg cactggcaag accttcaggt tgcagcaaaa
     4981 aatgaaagag tacacttctc atgctgttcc cgctgatcgt gatgcctggc tggattctcg
     5041 ccttgaatcg ttgaactgga tgcaggttat aacgctggtg ctgctcgatc ttgggaaacg
     5101 agcgaaagtt cgccaaatta ttgaacatat gtggtttcaa cgtaaggcat tattaaacgg
     5161 tcgtaatggc aatctatcga atactgcctg ggcagctttg caatcctata cagttcccga
     5221 gtcgttaacc gttgattata agaatcggcg tgagcctgcc gtatttaaca aaacagataa
     5281 cagcgaatgg tttctagttg attcacagct cgagcaagtg gaggatttgg tagagctcta
     5341 cgccgaactt aaacgtggcc ctaaatctgc cgaagccatc cagcgttttg cggtggttac
     5401 gttccaccaa tcttacggct atgaagaatt tattgaaggt atacgcgctc gctctgacga
     5461 gagtggcaat atctcttatc ccattgagcc gggtatcttt atgcgccttt gccaacgtgc
     5521 gaatgccgat ccaggacatc gctacgccat tttcattgat gagatcaatc gcggcaacat
     5581 atccaagatc tttggtgaac taatctcact cattgaagta gacaagcgtg caggcatgcc
     5641 caatgcgatg agcctgcaac tggcttatag cggtgatcac ttcagcgtac ccggcaatgt
     5701 cgatatcatc ggagccatga atacagcgga ccgttcttta gctctgatgg acacggcttt
     5761 gcgccgtcgc tttgactttg tcgaaatgat gcctgatctc tctttactga gtgaagctaa
     5821 ggtgaaaggc atagagctcg agtcgttgtt agagaaactc aatagccgca tcgaggctct
     5881 ttacgatcgt gaacatacgc tggggcatgc gttctttatg ccggtaaaaa atgcactcga
     5941 tgccggtgat gaagaagctg cgtttaaaca attgaagatc gcattccaga aaaagatcat
     6001 tccgctttta caggaatact ttttcgatga ctggaacaag atccggttgg tgctggcaga
     6061 caatcaaaag caagacgaca acctgcaatt cgtgattgag aaaaccgacg atctcgatac
     6121 gctttttggt aacaaccatg gtttacgacg ccatgatcag caatcaacag cttatgagct
     6181 caaagatttc gatcaagaga tctggaatat gccacaggct tatcgttcaa tttatcagcc
     6241 ccaacagact ccccttgatg agcaggcagt aaatcatggg tgaagttatc tccgtttttg
     6301 aatatgacct cttggggagt ggaaaagcgg cttcagttgg cgcaaaaccg gtgcctccac
     6361 aggtctttaa ttatttagaa gcgcttagcc tggcaagtaa tcaagggagc cagttcctca
     6421 aactcacctc tcgctcaggc ttcaaattgc ttcaggttca aaactacgca gggatgcttt
     6481 caacacctca cggtttccag cttgagatac tgcccaaggt tggtaaaaac ctcacagcgg
     6541 ctaatgcacg tcaaacattg ctgacgatgc taagccatct gcctgggttt cgacatattc
     6601 aaacccagca agccactctt caggctcagc gtatgccttt gcttgagatt tttattaacc
     6661 agtttttgca tagcgtcagc caattgctta aacagggttt acgctccgac tatgtgagca
     6721 agcaaggtaa tctggctttt atgaagggca agctgatgct ttctgcacag ctgcgacata
     6781 acgcggtgaa tcgccataag ttttgtgtcg attatgatga ctacatgcct gattgtgcag
     6841 ccaatcggct cttacactcc gcgctagata agcttcttag tctaaagctg tcatcagaga
     6901 atcaacgctg gctttacgaa ctgcgctttg cgtttgacgg tattccactt agccgggata
     6961 ttgaacgtga tataaacaac ttacgcctcg agcgtggtat ggctcattac aacgagccaa
     7021 tggcatgggc gcaattgatc ctgaggggaa tgagcccgag tgctttgcag ggaaacacca
     7081 aagcgatatc acttttgttc cctatggaag cggtatttga atcctttgtg gcacagaccc
     7141 taccctacga attaccttct cacctcaagg ttttacctca agcagcaact tattctttgg
     7201 taaagcatgg agttaaagat tgctttaagc ttcgccccga cttgctgatt caatcaaata
     7261 aaccagttca aaccgaaatg gtgatggata caaaatggaa gctggtgaat agcagccagc
     7321 agacaaaatc actctatgga ctggcacaag ctgacttcta tcaaatgttt gcctacggac
     7381 aaaaatatct tggtgggaat ggcgaaatgt atctgattta ccctgcgcat gatgacttta
     7441 gccaaccgat accgcagcat tttgctttct cggagacttt aaaattatgg gttgtgccat
     7501 accggataat ggcaaaacgc ggtgagagga tgatgtggcc gaatgacgtg tcctgtacaa
     7561 tgagccccaa actggatcgg atggctgctt cagtatccta tagtggaggc atgagatgac
     7621 ccatgaatac cagcgacaaa gcctggaaca actaataact cagcaaacag caactcccag
     7681 aagcgccgcc gttgtattta atgagaacca aaccctctgg caaacacttg gctttagcca
     7741 gtctcaagtt tcgctatggc ttgcctcatt gcctcaaagt aatcatctaa aagggcccac
     7801 aacggcaacg taccaggtta tacctgatat cgcggcacat ttggtatcat tgttgcatca
     7861 agctggtggg cgcatgcccc ttgcacaagt actgaaaaag ctccctgctg gtattacgac
     7921 cagtgaacag cagatacgca aactggcaca gaagcacgcg cagatagaga ttaaaggccc
     7981 cttgctggta ctgataaatt agccggcatt tgtagtaacc attggctgta ttaaaggatt
     8041 ttagaaaaca tcatggcaaa agaagcaaaa aaacaagacc cgcaacagca gctagaagca
     8101 ctgttcaata aactggtcaa acagcactcc gagtttactc agccattggt cgaattaccc
     8161 gagcatgaat tcgccaaggc tggtctcatt caaggcctgg ttgaaggcaa cttaacgcca
     8221 ccagtcatgt tgattgcgct gacagaaggc cattgggaat acgccgctga cactgcaaag
     8281 tttgatgatt tagcctatca gctgttaagt gatatcgaag gtgactggcc agtatatgcc
     8341 gttgtggacg atggcttgaa ccaccgtatt ttggccttgt tcggtgccga tggatctgac
     8401 ggtacccacg gtgcagatac cctgcctggt ctggaagaat tacgtagctt tgatcgcatg
     8461 gagcgtgatc caacctttcg ctggtcgatg cgtgtttata cacgcttaat gcagcgattt
     8521 gatgccttcc atgaaaatgt ttatcgcgtt accaaagaca aggtaaacga caagaacgac
     8581 attattgagg aagtagctaa gcttctgttt ttagaaactt tccgcctgca tcacgatgaa
     8641 gaccttacct ttaaagatga tgagggaaat accctgcgtt ttcgtgatgt ctttaattgg
     8701 caatatgtgg agagccacgg cgataaagcc gttgcccaaa tcaaagccgc ctttgaggaa
     8761 tttaaaagcc acgaaaacta cgttgtgatc tcagatgatg gctcacgtaa ccccatcttt
     8821 agtaaagaaa cacacctgcg gctgtctgtt gcgaaaaact accaggattt gctggaagca
     8881 atacaaaacc tgggtccggt aaaaaccaac gacggtaaaa tcgctaaaga acacggcacg
     8941 ttggctgatg tgtcaggaga tttactcggc agagtgtttg atgtattcct acgcgctaac
     9001 tttgaatcca aaggtggcct tggcgtttat ttaacaccta acccagtaaa acaggccatg
     9061 ctagaaattg ctatgcacga catcgacgat gatgatgaaa tgcgatcgcg cttagccaat
     9121 ggcgattttc gtttttgcga ccccacctgt ggctcctttg gttttggctc ggtggcgtta
     9181 agccagattg ataaatggat cgattttaag ctggtgctgg ccgacgataa aaaagaatca
     9241 ttgaaacaga aattacgcga ctgtgcattt accggtgccg atgctgcacc acgcatggta
     9301 atgctggccc gcgtgaatat ggcattgcaa ggtgccccca aagcgcagat tttctatacc
     9361 gacaactcac tcactactaa cgccctgaag ccgaatagtt ttgaccttat ttgcaccaac
     9421 ccgccgttcg gtacgcctaa atttgataaa ggcaaaaatg gtcaaaactc aaaagccaat
     9481 tacgaagcca atatggagca agtgctcggt ggttttcgcc cgaccaaaaa agtcgttgat
     9541 agctacaacg cctggtatga ccacgtaaaa atgaagtggg aggatatcgg tgatttagag
     9601 ctggatgaaa acggcgagcc taaatgggcg ggctatcgta ccgatttgcg cagaacgggc
     9661 gggactgata aaaagccgtt ttacagcctg cagcctacaa ccgcagggct cgcgctgggc
     9721 agcaagcccg atagcaaggg caactggcaa ccggtaggtg ccactattga cccagcagta
     9781 ctttttatcg accgctgcct gcaactgctt aaacccggag gccgtctgtt aatagtgctg
     9841 cccgatggta ttttatgtaa ctccggcgac cgttatgtcc gtgagtacat tatggggaaa
     9901 aaagacgaaa aaaccggcga gtttgttggc ggtaaagcta ttgtaaaagc agtaatttca
     9961 ttgccgagcg actgctttaa actctccggt acgggggcca aaacctcgat tttgtattta
    10021 caaaaacgcc atgctaaccc caaccagcca gagcaatttt tgcccgagcc acaaaccgac
    10081 gtgtttatgg cagtggctga aacattgggt tatgtggtga aaaacaatat cgaagactat
    10141 aacgccggtg tcgcaaacga tctggataag attgtatcgg cttataagcg gggtgagtga
    10201 tggcaactat ttttaaaaga acacccgaac aattgtctga aaggcggatt gatccttggc
    10261 actatcaacc gcgatttgaa agacaaatta ataagataaa agacaaccta ggtgctttgc
    10321 ccctagaagc ggtaattgat tcaaagcgcg gggtggctag tggcgcaaca cctttaggag
    10381 caaattattt agaaaaaggc cgtgttaagt tttatcgcac ttctgaagtt gaagaaatgt
    10441 tcttgcaagc tgaagaagct gtttttatta acgatgaaga tgatgaaaaa ttaaagcgca
    10501 gccgattagt ggatggcgac gttttactta cgattactgg tgcaaaattt ggtaaatcgg
    10561 ctgttgtgtc agctaaacat ctaccggcca acattagtca gcactcagtc agattcaagc
    10621 ccgatcccaa aaaagttgac gcctatttct tggtggcata tttgaatagc aaaaccggac
    10681 aagtagcgat ctggaaggaa gcatatggcg caactcgccc tgcaattgac ttcccaagtg
    10741 tccgctcgct agcggttcct aaagttttac ctctagccca aaaatacatc ggggataaag
    10801 ttcgccaagc cgagcagtta cgcgcttggg cgaaacgact tgaaggaatg gctgatagaa
    10861 aaattaaaga tttatttcac ttcaaccttg ttgattcatt aacattgaaa cctagaagaa
    10921 tgaaacaaca ggtattatca gcagtgtctt tagctcctga gtttgcacgt gcggccgata
    10981 gtcaaatgac ctttaggaat tcatcaaaac tatctaattt cattagtaaa tgcaaatgtg
    11041 gcgaccctat taaatcggaa gacagagtac ccggaccata tttttactat ggcgcatcag
    11101 gtccaataga tactcatacc gaattcaatt tcaacggtaa atatctgatt attgcgcaag
    11161 atggctctat tggctgtgca aatgtagctg atggaaagtt ttgggcaaac aaccatgttt
    11221 gggttttaaa ggtaaaagat gaatatgata ttgagtctat ttgtaggttc ctggataaac
    11281 actttccgtg ttggaaaggt gtgactactg gcagcgttgt accaaaagtg acttcagaaa
    11341 acctgttaaa tattctgatt ccgattgaca tagcgaagaa tagagaaata ggaagtaagc
    11401 tgagactagc cgttactacg gcagcatatg caaaaaagtt aacagcatct gccaaaactc
    11461 tagttgagtc attaatcgaa ggccaactga ctgagcaaca actaatccag gcccaacaag
    11521 ccctggaaga tggcgacagc agctttgatc aagcgatttt aagcaaactg agcgctgagg
    11581 gttatgccat tgaaggtgcc acaccgctat ttagcgatgt ggatgagtta tacagtttgc
    11641 tggaagaggc ggcgcaagcg gaggctgaag agtaaagatg gagacaaggg gcacggacag
    11701 cagtacaacc aggcaatata acgaccccgt aaatagcgaa gcggcactta acctctgcct
    11761 gcagctttgg caacaaggtg gtttgaacgc caacaaagca gcattgttat tggcggctgt
    11821 gcctgccctt aggtcactgc tacaaccaat aatacagccg aaaaagaacg acgctgaaac
    11881 ggatatcgtt agcgccttta gcctcactgc accattgctt gatgccttta acgatttaag
    11941 ccagtcaggt gaatggcaat tagcgttgtt gggcttaaac ccagatgttc gccaacattg
    12001 gatcaattta gccgccgctc gctgccagga agcaggcacg atgagtgaca caatggtatt
    12061 ggttaaactc attcagcaac tgggcaatgc cagtgaatgg gtcttagccc agctggaaag
    12121 cactgccacc acgccgcaaa tcattgccgg ccctctggca caaaccgaac gcgatttgct
    12181 cggtcatagc ctaaacgaca atgctgccat tcccgcgcta tgtcgtattt tgcgcaccag
    12241 ctataccttg tttaccgtga gcgaacaaaa tgaaccaccg gcacctatac aagcggtgga
    12301 tgtcaccgca aaacaactga caaataactg gtgtagtggg cggttgttag cactccctaa
    12361 taccttgctt gatgaacata accttaaacc caatgccgat tggttattgg tgtcccgctc
    12421 gggtcatgac aacatgccgc ttaccgagct attcgcccaa cagccctggt tatttttact
    12481 cagtctaatt atctttgtgc aagatgcctg ggctgccgag caacgtggcg gtttgttgct
    12541 caccttacct gccgggcaaa atgcctttgc ccccggtcaa ataaatgtgg cggtacaggg
    12601 cattgaaggt gatgaggtga gtctgggtag tttggccgag tttcttgtgc ttctgctcgg
    12661 tgagctcaat atacccctct acccggcact cgatgcgaac acggaaagca tcaaccgcct
    12721 aaaccgcgtc ctgagctcat tcattgccga attgttggca aaaaaaatct ggcaatttac
    12781 cgaggcgggt cgtggagagg ccggccaata tcgaattcat acaagcttca gcgatgcctg
    12841 ttatagcctg ccgttagcac cactatttgg ctacaaaagc caaacgttac aacggaccat
    12901 taagcaactg gcgcaaaact gctatgccaa taaaaaacgc gccgccaata gaataaacct
    12961 tcaaggatcg tcgttatgag ttttgaatta aacgaacaga tggttgatag cctggcaaaa
    13021 caacatcgct gggcggcgac gggcgtatca gattatccac ctcgcgaccc gttagtgggc
    13081 cagagccgct tttttaaacg ctttcaaacc tttttgcata cggtggatca cgacgatgat
    13141 cgctttgccc atgtgtttgc cgtcgaagcc gaatggggtc ggggtaaatc acgtttaggc
    13201 catgaattgg tcgcacaaat taacgactgt tcaaggggct ggtttgtccg cgacgaacaa
    13261 ggccaactgc acgataagca gctgtttaat cgcgacacgc aagacaaata cctggcgctc
    13321 tacattcgct actcgcaagt ggcatcagat tatcaaaact cggataactg gtttgctttt
    13381 ggtttatatc aggcgctatt accactggcc acccaaacct ttgatggatc gattcaaagt
    13441 gaaattgcca aacaagccta tgaacgctta atgccatttg gctttaaatc tgaactgtta
    13501 gcagatgcct tacagctgaa cgccaaccac agcgaagaag acctctatac tgaccaaacg
    13561 ctggtagtca gtttggtgca agccgcctat aaagtattaa agagctttgg cgtgcagtat
    13621 atgttggtgg tgctggatga gctggaaacc gttgcagaag ccgccacctt tggcctggaa
    13681 gatgaacaaa ataaacgcct cgatggccag gccattcgcc tgattggtaa agcaattaaa
    13741 gaggaagacc ctcgccgtaa acttccatgg ttgcgctatg tagcgctgtg ttcaccactg
    13801 cttggccagc aattgcgcga aattcaatcg gtcgcacgcc gctttgagtt agtggaactt
    13861 gagcataatg cctttgccga tgtatcagac tatgtggcaa gcctcttaaa agacggcaag
    13921 ctgggtttta attaccctgc tggtttagtg gaagctgcat acgccatgag tggtgccaac
    13981 ttcggttggt ttaacgtggt catggctaat gtggatgcag tgctgagcca ataccaacaa
    14041 gcaggccgca gtatcaagga tgtgggcgat ttatttgaag ctgtgctggc aggctctggc
    14101 cgcgtagcca ggcatgtgct ggataaaggc gcgatagaag gcattcaaac tcgcgaccag
    14161 gcactattga ccgaatgtcg tagtttgctc tacggccaat tacccgtgcg cttagctgaa
    14221 atgggctatg ccacagaatt gctggcgctg aaaaacgaag atctggaacc catagccgct
    14281 cattaccgca aactgagcat tgaccgccta cagtgccgac aagccctgga gcaagccaaa
    14341 ttccgccgcg ataaagatga atggtactac cctgcagtag atcaagcgct gaacttagat
    14401 actttgctgg ataacctgcg tactttttca attaaagaac aagccgcaag cggcgataaa
    14461 ggcgcggtat tactacctct gtcaaaaggt gagtttaaat acttactcag cttgctgtat
    14521 gaccacccag cgattgaatt cgctgccgat gccttatggc acaagctagt gggtgaaact
    14581 accgaactgg acgccagcga agcaacgcat attggcccca gtgtggcgat gttactgcgc
    14641 ctggatttgc gctatcgcag tgcccagcaa aactcactga tttttaaaga tcccgccatg
    14701 ggcgacgctc atgaacaggc aatgagtgaa cttagcaaac aaagtagcca gaagccgctc
    14761 atcaaggcat tagcccgtgt aacgggcttt gtgagattac ttgataacaa ctggacctat
    14821 gatgaaaact tgctgagtaa taaagcgggc agcgacgatg cacctttggc cattttaacc
    14881 gagccccgtg gccgccaggg tggtttactt accctggatg gatttaagct tcacccagaa
    14941 ggtaaagcct tgtttgcctg ggtcagcaac ctaattgagc tgaacaatct tcacaccttt
    15001 gcggcaaacc acagccacca aaatggtcgc acgccagtat tagcgttaac tgcttctgca
    15061 catctcatgg agcaatacag ccgactggat gaaagaaatg agttgcgtga tgcgatactg
    15121 ctgcattatg tcaacccaag cgaagcagac cagttagagc gtattggctt aagtttggca
    15181 gcatgtcagt tgcacggcgt gaatttaact gccgacagct ttaccgcgaa attcaaaaac
    15241 aagctgcatg ccctgacaac ctttgccacg gatgccattc acaagtggcg ccagcgtttg
    15301 cagcagcgcg gtttaattgc ctggccgtta aaggtagatg gtaaattatc accgaatgac
    15361 cgagacctgt tgtttaaagg ctggtatcag ctggccattg ctcaccctga gttaaatggc
    15421 attctcgatt tacagcagca acacggcgtg ccagtgaatg agctgagcag cttgttggat
    15481 aaacttaagg tgccgggcag ctatattgcc aaaggttaca ctgccgatga acatgccggg
    15541 ctatttactg aacttaataa tgtgcagcga agccaggcgc agatcccatt atttttagcc
    15601 cgtattgctc accctaataa aaagcacaag tggcaattcg aaggctttaa gcaacagttt
    15661 tatttcgcct atgtggctga aacgagtgtt accgccaaag gcgtgtttaa tgactggatg
    15721 tggtggtgtg gagagctgaa cttactcact ttaaccaatc caacagaaaa acaggcaatt
    15781 tgggagcact acccgcgctc ccgcttagaa aatgccatta gagaagcgca aaactggttc
    15841 cgtggtaacg atatgggcag ctatgcagcc aatgtggaag tcatgagccg cgtgtatggt
    15901 tatgcacgca ttaatgaaat gttcgcgcca ctgggtaaaa ataagctggg tttcgtcacc
    15961 gtggaagcta aagagcagct ggaaaaagca caatcgctat ttaacgtgct caaacagcaa
    16021 gaagaacaat tggctgatat ggtcgaggcc aacgacacga aagtattggc agggcttatc
    16081 cacaaacgcg ctgaagtgct ggagctggta gcaaaggtta aaccgcttaa tagttccagg
    16141 cctatgctaa aagatgccca tattctcagc ttagaagaca aaacgacctc gctttatcaa
    16201 cggattgaac aggcctgttt atttgccgag tttgttgagt gcagcgctga gcgtatcaac
    16261 aaccgactcg ttgatttaat catagatgtt gaaacagagt gtgccccgct gacgaatttt
    16321 ccgaaacgtc tgtacaccaa cacgctgcgc accataggtc atattttaga tggagcgtta
    16381 aaagacgata ccagcagtgc cactggccga aaagagcagc aagcagatag cgatacctta
    16441 ctgcattacc tgcgtaaact cgatttaggt agagcgcatg acaagctttc agccttagcg
    16501 caagaagtgg gtttaaacct gcacaatgat caacagttgc cgatagcgga gattcagggg
    16561 catattttgt ccagctaccg caactgcaaa gaacgattta gtaagctggt taataacctc
    16621 acagagcaaa aactacgtgc acaacaactg caagaatggc ttagtagcgc cacggctgag
    16681 tatcagttcg ccgatgacat tgcagagcta ccaaaactgg taatgaaatt acagctcatt
    16741 gaagatgcta ttgccgatct ccccagtgat gcagagagca agcgtcaaag tatgcaaaac
    16801 agtttgcgca atgggcagtt ttcatcgtta cgggatttac cagaagagct tttaaaaccg
    16861 gcacgcggtc aacttacacc gattcaaggc cagctactta aaattgaaga gcgtctaaac
    16921 caagtaagac gcaactccat cgcacaggta aacagctggc tgccgttatt aaaaccgcta
    16981 ttggcttctc aaaagcaagc ggaaccagca gcgttgactc tggaggatgt gagcaatttg
    17041 ggtgtcagag aacttcaaca ggtgtgtgaa agcacccagg ccaaatggca gagtcaaggt
    17101 gagcaaatat taaatgatac aggcttaaca ctggcggatt ggcaaccgat ttataaggct
    17161 ttgagccaaa atcaggaacc cgtgttaaca cctgagcaac agaaaggact tgtagacaaa
    17221 ggcattgtaa aaatgcgcct tacctttgcc actggtttgt agaggtgacg cgatgagtcg
    17281 ttatttatct gcggcactgg ccagtaatcg taaaggccgt tttttgcaaa ctgttgcggg
    17341 ggctaccccc ttaatgaagg attggattag ctcgccgcca gccagcggtt tgttaatagt
    17401 gcaagcagaa gagttgactg acgctaatac tatgcagcac ctgtatcact gggctatgca
    17461 ggcaggttgt gccgctttgg taataaacct gaaagcagaa caatttacct tattagcgca
    17521 attgccctat ccactggatt ggcagttagt accagcgagc ttgagggggc aggaaccggg
    17581 gctaaccgct ttgctagcca gtgaaacaga ccaggctatt gctggcttta ctggcagcgc
    17641 agatcgctac cagcaccagg ccggtgatgt agtgcatacc cgctacatac gaaaacacag
    17701 caacagtggg ctattagcct ttaccacgtt acctttgtgg tcgctgactt tactcgatca
    17761 tagtgagctc ctggtgagct ggttgaattg gtttgtggac catgcaggca ttgctgaacg
    17821 aataatcgaa cctaaagcgc catcaactga ctacacgcca gataagcacg atctggttgt
    17881 attgctatta ctctacgctg gcggtggcat gaacttacaa gcgttgtctg agcataatgc
    17941 ggttaagctg atgtttgatg ttaatagcct tgatattgtc aagcggggtg agatgttgcg
    18001 gcagcacgat tttatcgacg acgcgggcat aacagctacc ggtaaaacat gcctacaagc
    18061 ctcgcaatat tgggcttatg ccccattact tggcgagcag ctacacacag gaacgctata
    18121 agcatgaatg agaacaaaac gatgaattta gcgcaatcgc tcgccatcga tagccagctt
    18181 cgcttaccgg gaagtgcagg acgcttgctt aaacaggtcc gtgatattga gcaagtggcg
    18241 ccgctattcc gtgatgctgg cttagtgaaa gcggtcccca agcccacaat tgttaattta
    18301 cgcgtagccg gtattgcgac taaatcgtgc ttagacaagg tattgggtgg ttttatttat
    18361 gccagtgcag cagtgcgcac agatttgtta atagatgaca ataaagtcag cacggtgggt
    18421 agcgactcgg tatcagaact ggatgatatt gattttgaag ctcagcgtaa acggcttgat
    18481 ttaattgaaa ttcgtcaggc ttatcagtta gttgagaaaa aacttttagg ttatgagcct
    18541 ggcgatttaa ttttgctgga tacgccgttg tttttagcac gggatatggc tccgctggag
    18601 agaaatgtcc gtcataaaca agagtacgaa aaaaccagac aagtgataga aactttttgg
    18661 caaaaccatc gctccagaat ttttccctgg aatcaaaatg gtgtagtact tgcgagcatt
    18721 ttggctgagc gctttagtgc cattgtaagt atcgccaaac aagatttacg taccgaacaa
    18781 ggacgcaaac aaattttagc atctgatggg ttttctaaag agttgatgca aaatctggaa
    18841 ggactggatg aaagtttggt tggcgtggga gatttgcgat ttatcaatgg cattttaggc
    18901 aactattcgc gcaccatcgc atttcgtttg tctgaacaag aaaagcgcat tgaacccaat
    18961 attgaagcta agcaaggtct aattggtttt cacttccgta gtgcccgagg tggacagata
    19021 aaaatggtgc aattagcggg tgacgaacca gaatggaaaa gtgctgatct cgatttagtt
    19081 gcctctcgcc tgatggtgct ggatatgcaa aacaaaggta atgcaatgcc gttgccacaa
    19141 ttacttggtt atcaacagct tggtatttta cctaaatttg ctacgtttta tcgccagggt
    19201 ttacaccagg cactaaagaa taatgatatt gaggcagggt ggttggcagg cctcgatgag
    19261 ggattgaata atggcttata aagttttagg taaattagtc ggtaacactg gtgatgccag
    19321 tcagttaact atggttgtac aagactcgtt ttcagtacgc cgtggtgagt ttgtacgcat
    19381 tatgcaccag gaacgcaaag atgaggggca agtcgccgta ctggggcgag tgaccaagat
    19441 aagccgtact aatatgctct ataacgccgg ctttggcgat ggtgtaaccg agcttgagct
    19501 tttgcccggt gcgaatgtaa ctggtgagaa tatgtttgct caggtcgagc tggttggtta
    19561 ccgcgatcct gttactcgtc aaatcaaaat tccacgtcgc ccgcttaacc cgggtaccgc
    19621 tgttgaaacc gtcgattttc agtttttgag tgatttttac gaatttaatg aacactccag
    19681 tttgcactta ggtaacttag taggctacga caaaggtgag aatacagttc ctgtttttat
    19741 tgacgtgaac aagttggtca ctgagcattt ggctgttctg gcaatgacgg gttcaggtaa
    19801 gtcttataca gttggccgca ttattgagcg tttagttgcg gttaataacg gtacggtagt
    19861 agtatttgac ccacatggtg aatacggtaa agccctcgct ggcggtaatc tgcaattctc
    19921 aagcttctta gaagccactg acgataagcg tgatcaagaa gcactgccac tgataaaaca
    19981 gacctttgaa aagctgcaag ccgccggtgc aggtattcag gtttactcgc cgcagcatga
    20041 atcttttaaa cataaatacg ccagtaagaa tacgccatta gcgcttcagt ttgaccattt
    20101 tgaaatggac gatattgctg aaatattacc tggcttaacg gaaccgcagc aacgagtact
    20161 cgatgttgca attcgttatt ggcgctctgc agataagact gagccacgag atattaaccg
    20221 cctacgcttc ttattaggtg atggtattga agagttaaaa gagtgggacg acctttctga
    20281 agctgaggcg aaggcgttat caggccgtag tgccgccgtc gcctcgatga agctttcacg
    20341 tgtgcttaat gaagctaaaa gtttttacag cgcaacaatg gctgagccaa ctgatattta
    20401 caaaatggtg ggtcgcccga gcaatcaaca ggggcgttta gtcgttgttg acttacaagg
    20461 tttgagtgat accgctaagc aagtcatttg tgcactgtta tctagtgaaa tattaaaagc
    20521 agcctcgagc aaaacggacc cgctgcgccc atgctttatc atttacgaag agggtcataa
    20581 ttttgccccg gccggcggca acgcagtcag tcaccgcatt attaaaaaga tcgctggtga
    20641 aggtcgtaaa tttggcgttg gatttggcat tgtgagccag cgtccatcga aattagattc
    20701 agatgtgacc tctcagtgca atacgctaat tacgatgcgc ttaaagaacc ctgacgatca
    20761 acgatttata gctaaagcat cagatatggt gagcaaagct gatctggagg aactcccgag
    20821 tttatcaacc ggtgaagctt tggtgtgtgg tcgttcaatc cctgcaccac tgcttgttaa
    20881 agttggtagt aaggcattaa ttcacggtgg tgaatcacca gaggtactgc gtgtttgggg
    20941 ctctttcagt ggctaaggtg cctatgcctg aagttgcctg ggtaaatgag acatttccac
    21001 ttttagcaag cggtaagcta ttggaccagg atgtattggt ccacagcctg ggctgtagcg
    21061 tttggaatac attcgggcat gaacagtcat ttatggcggt agtggaatgc ccggctccag
    21121 ggacatttgg tgctgatatt cgctctgact cgggctggtt tcataaatct tctgcatcgc
    21181 ctgtgtgttt aatcgaattt gaacgctttg atggttcagc gaaggggcag caaaagctgg
    21241 aagaaaaatt aaaaaactta ctggaagcag cacagcgctg gaatcattgc cccaaaaccc
    21301 tggtcctcag tgcctggagc caaggcttag taggtgtacc tgatactcaa aaactgaaag
    21361 acatatgccg tatgggtttt acttcgtcca cgggtaccca agtaatcgcc gctcctgatg
    21421 ttgaggtggt atttagtcgc tttttattta tcaaaaacct gaacatgatt gttttggaca
    21481 gaattcacta tgaggtgttg atgtgaatat aaaatcagca cattttttgc cagggacgaa
    21541 taaacgcctt ggtgacaaat acctgttact tgaatgcctt ggtgatggca gccatggctg
    21601 ggtatggcgg gcggaacgcc tgaaagatgg aaaaattgtc gcagtaaaaa tccctaagga
    21661 tattactcgg gaagaccgtc agcttgccga aggtaaagag cttttaaacg tcgagccgca
    21721 cgaaaatatt gtgcaaattt ttgatatggg ccgtatagat aatgaatggt tctatattga
    21781 aatggagtat tttcccagcc aaaccctggc gcaaaagctc gatgacaggc agcgtaattt
    21841 tggccagacc tatgaacggt tgttcagaat atatcgtcaa gtgctctgtg ctgtgcatta
    21901 tttggccgaa ttaccagtac caatatctca tggagatatt aagccacata acattttggt
    21961 tggagagcgg gacctggtga agttgacgga ctttggcagc tcagctttac cagaagaaat
    22021 ttacgttcgt acccgtgaaa atggcgggac tgtgctgtat tcggccccag agttctccaa
    22081 cgtcgatagc cgtagaggca gcctggaaga gctactattg ggcgacatct atagcttagg
    22141 tgttttgcta taccagctgc tgacaggaaa gctgccccat gatacgccag cacaagttca
    22201 gcgccacgca cctttcaagc ttccgacaga gattaatagc tctattcata cggacctgga
    22261 acaagtggtt cttacttgtt tacaaaaacg agcggaggat cgcttttcca ctatttccga
    22321 cttaattcat gcatttgatt ctgctgccac taagcaatta aaagttggtg ctattgctcc
    22381 cgtttttcaa actaaacctg ctcaggactg gtcaagtgcg gtacttgaag caatgtccga
    22441 tcaatcttac caaaaagctg ctcaattagc gtcgcaggag tatagccgca gtaaagatct
    22501 acaagcttta catcagcaac ttattgcctt gtatcgcgcc aatcgcttat tcgactttga
    22561 aaaagttgta gaagataata aagcgatctt acttgagggt aaattgactc aaccagcagc
    22621 tttgttcgag ttgatagtta aggcaaacct acagttacgc aatatcagtc aagcaaattt
    22681 ctggctgttg caacgaaagc aagctgaagc agaaaacgcg gccaccatgt atttagaatc
    22741 atctatcctt ggccttgaag cgaaatattc tgaagcaaga gtactattgg agaaagtaaa
    22801 ccaaacaact ccaatgaagt tccatgtgtt aagccaactc attttagttt gcgagcaaat
    22861 gcgtgattac tctggtgctg cggcttattt gaaagcggca ttgcgagtag caccattgga
    22921 taacagcatg aaagagaaaa aagaattgta tgcaaagctt ggagttattt gattgaaagg
    22981 atgaccattg cgcgaacaag gcatagtgga tatttaacac ttccgttcac ataccggaca
    23041 aaaaaataaa gttttacata acgatttcct tcttgattga gttaagacaa aagcctctcc
    23101 ccattagggc ggaggcttct cagttataag cctttaatta aaaaacagtt aatattcata
    23161 tacttagata gaattatcga aaagaactat taatctttgc atagtcaaat ggagtgatag
    23221 ccgccattct attcgcatcc agataatcag cccaccactg cagcatcaac attcgctccc
    23281 caagatgttc cgccttatgg atataagccg cccgtactga gctacgttcc tgatgactca
    23341 tctgccgctc tactgcatcc ctcgaccaca atcccgattc aatcaaagaa ctacacgcca
    23401 tcgtcctgaa accatgtcca cacacttccg ttttcgtgtc atagcccatc actcgcaagg
    23461 ccttgttaag cgtattctca ctcattggct tacgcggatc gtgatcacca acgaagatca
    23521 gctcacggtt tccactcatg cttttgattt tttccagaat ggtgagagct tgtcgagata
    23581 agggtacaag atgtggagtc cgcatcttcg aaccacgctg agaatgctta actccctcca
    23641 gtggctcacg ttctcccgga atcgtccaca tggcagtatc aaaatcaact tctgaccagc
    23701 gagcaaaacg caactcgctt gaacggatga aaactaacaa tgtgagttca accgccagtc
    23761 gggttaacgg cctgccagaa tagtgataga tgcgttgaag caattcagga atgcggttaa
    23821 gttccagagc agcacgatgc tgtcttttcg ccgtagctac cgcaccagca atctcttgag
    23881 cggggttgta gtcgattaat ccactctgta cagcaaagcg tataatcgcg gtagtatgct
    23941 gctgcaaacg agcagcgact tcgagacgcc cagacgactc caccgctttg atggggacaa
    24001 gcagatcccg agtcttcaga tcagcaatgt tccgcttacc gatagcagca aagagattat
    24061 cttcgaggct tttcaataca cgagcgctat gcgatgcaga ccacttctga ttgctggcgt
    24121 gccactctct ggctaccact tcaaatgtta tcacctcttg ctcatgctct accttaacag
    24181 ctttcttgtt ctcactagga tcgacgccgt tagctaacaa cttacgggct tcatcccggc
    24241 gacctctggc atcgaccagc gagacttcag ggtatttccc cagcgccagc atcttctcct
    24301 taccgccaaa acgataacga agtcgccagt atttggagcc attagggtga accaacaaaa
    24361 ccatgccttc accgtcagta agtttatagg cttttgcttc cggcttagcc gaacggacct
    24421 tcacatcact cagagccatg atgagtatcc tttcaagggc cctgtgtggg tacaaacata
    24481 atcgaaccgg gaaatacccg cagttgtacc cgcatcagta agttgatgta gattgaatca
    24541 ggttgactta ggttgagtga aaaagcgagg aaagccttgc ggatactgga tttcaggcac
    24601 aaaaaaagcc gcccttgagc gactcgattt gcatacgatg tggtgccgaa ggccggactc
    24661 gaaccggcac gtatttctac ggttgatttt gaatcaactg cgtctaccga tttcgccact
    24721 tcggcactga agaggtatgc ggaaaacgtt gtggattata cctggcgcac gccaccatgc
    24781 aagcgccagc ttgctcccac ttcgctaagt gctgaaattt tcagcattgc atacagcgat
    24841 gtgtaacctt tgtcacactc caggcacccc gccctgccat gctctacact tcccagacaa
    24901 caccagagaa ggatcaaaaa atgtcgatga taaaatgcta tgccgcaaaa gaagcgggcg
    24961 gcgaactgga agtttatgag tacgatcccg gtgaactgaa gccacaagat gttgaagtgc
    25021 aggtggatta ctgcgggatc tgccattccg atctgtcgat gatcgacaac gaatggggat
    25081 tttcacagta tccgctggtt gccgggcatg aggtgattgg tcgcgtggtg gcgctcggga
    25141 gcgccgcaca ggataaaggt ttgcaggtcg gtcagcgtgt cgggattggc tggacggcac
    25201 gcagctgcgg gcattgcgat gcctgtatta gcggtaatca gatcaactgc gagcaaggtg
    25261 cggtgccaac aattatgaat cgcggcggtt ttgccgagaa gttgcgtgcg gactggcaat
    25321 gggtgattcc gctgccagaa aatattgata tcgagtctgc cgggccgctg ttgtgcggcg
    25381 gtatcacggt ctttaaacca ctgttgatgc accatatcac tgccaccagc cgcgttgggg
    25441 taattggtat tggcgggctg gggcatatcg ctataaaact tctgcacgca atgggatgcg
    25501 aggtgacagc ctttagttct aatccggcga aagagcagga agtgctggcg atgggtgccg
    25561 ataaagtggt gaatagccgc gatccgcagg cactgaaagc actggcgggg cagtttgatc
    25621 tcattatcaa caccgtcaac gtcagcctcg actggcagcc ttattttgag gcgctgacct
    25681 atggcggtaa tttccatacg gtcggtgcgg ttctcacgcc gctgtctgtt ccggccttta
    25741 cgttaattgc gggcgaccgc agcgtctctg gttcagcaac cggcacacct tatgagctgc
    25801 gtaagctgat gcgttttgcc gcccgcagca aggttgcgcc gaccaccgaa ctgttcccga
    25861 tgtcgaaaat taacgacgcc atccagcatg tgcgcgacgg taaggcgcgt tatcgcgtgg
    25921 ttctgaaagc cgacttctga tccctttgcc gggtgcctgt cgcccggcac ttaaatctct
    25981 ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc cgttgcctat
    26041 actccaggca ggaaactgga ggatatctca tgagcgaacc cctgttaatt gcccgcacgc
    26101 cggacacaga actgttttta ctgccgggaa tggctaaccg tcacgggctg attactggcg
    26161 caacggggac gggtaaaact gtcacgctgc aaaaactggc ggagtctcta tcggaaatcg
    26221 gtgttccggt gtttatggcc gatgtgaaag gcgatctgac cggcatcgcg caggcaggaa
    26281 cggcatcgga aaaactactc gcaaggctta aaaatatcgg cgtcaatgac tggcaaccgc
    26341 atgctaatcc ggtggtggtg tgggatatct ttggcgagaa aggccatccg gtgcgggcga
    26401 cggtttcgga tctggggccg ctgttgctgg cgcggctgtt gaatctcaac gatgtgcaat
    26461 ctggcgtgct gaatatcatc ttccgtattg ctgacgatca gggattgttg ctgctcgact
    26521 ttaaagatct gcgggcgatt acccagtaca tcggcgataa cgccaaatct ttccagaatc
    26581 agtacggtaa tatcagtagc gcatcggttg gtgccatcca gcgcgggctg ttatcgctgg
    26641 aacagcaagg cgcggcgcac ttttttggtg agccgatgct ggatatcaaa gactggatgc
    26701 gtaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaag ctttatcaga
    26761 tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agaattgtat gaacaattgc
    26821 ctgaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgaa gcacatctgc
    26881 tgtttaacga tgcgccacag gtactgctgg ataagattga gcaggtgata aggcttattc
    26941 gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtcagatatt ccggataatg
    27001 tgctcgggca gctaggtaat cgcgttcaac acgctttgcg ggcttttacg cccaaagatc
    27061 agaaagcagt aaaagctgcg gcgcaaacca tgcgggccaa ttcggtgttt gataccgaaa
    27121 aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat gcgaaaggaa
    27181 gtccttcagt ggtggaacgg gcgatggtga ttgccccctg ttcgcgaatg gggccggtga
    27241 cggaagatga gcgtaacggc ctgattaatc actccccggt gtatggcaag tacgaggatg
    27301 acgtggaccg ggaatccgcc tatgagatgt tgcaaaaagg ctttcaggcc agtaccgagc
    27361 agcaaaataa tcctcccgcg aaagggaaag aggtggcggt ggatgacggt attctcggtg
    27421 gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa gatggtgtgg
    27481 tgcaaacaat ggcgaaaagc gccgcccgcc aggtgacgaa tcagattgtg cgtggtgtgc
    27541 tggggagttt gctggggggg agaagaaggt aagtcaggtg cggtctgtgc ccctcaccct
    27601 aaccctctcc ccaaaggggc gaggggaccg atcgaactcg tatttgactt tatcaacaat
    27661 gtgaaaggga gctttcgctc ccttgcttgg ttacgatttt ctcattaaca gccacaggct
    27721 gattaagaag aagctggcgc ttggcaacag tgcgccgata atcggcggga tgccgtaaac
    27781 caacgtcagc gggccgaaga tctggtccag tacgtagaag acaaaaccga agctgatacc
    27841 ggtgaccaca cgcacgccca tcggtacgct acgcagtggg ccaaagataa acgacagcgc
    27901 catcagcatc atcaccgcca cggatagcgg ctggaagatt ttgctccaca tgttgagctg
    27961 ataacgtccg gcatcctgac cgctcgactt cagatacttc acatagttgt gcaaaccact
    28021 gatggagagt gcatccgggt ccagcgccac cacgcccagt ttgtctggcg tgaggttggt
    28081 tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg tcagatcaga
    28141 ttcatcaacc tgcgacagac gccagacttt atgttccggg tcaaacttcg cggtagcggc
    28201 atagcgtacg gattgcagac gacgattctc gttaaaggca taaatactga tgccacctaa
    28261 ctcttcgtca cctttaaccc gctcaatgta gacgaagttg ttgccatctt tcgcccataa
    28321 gccttgctga gtagagagca acgagccgcc gtacatcgcc tgcgcacggt agttacgcgc
    28381 catctgttcg ccctgcggcg cgacccattc gccaatcgcc atcgtcagca agaccagcgg
    28441 aatggcggtt ttcatcaccg acagcgccac ctgcatacgg gtaaaaccag aagcctgcat
    28501 caccaccagt tcgctgcgct gcgccagcat cccaagacca agcaacgccc caagcagagc
    28561 cgccatcggg aagaagattt gcacatcttt cggcacgctc agcaaggtat acattcctgc
    28621 gcctaacgcg tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa acttgataat
    28681 gcccgacagc gacaccagca tgaacagcgt catcatgatg gtggtgaaaa tagttttacc
    28741 gatatagcgg tcaagtacgc caaaaggttg catcacaccg ctcctttacg cgaaaaactg
    28801 gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc caaagccaga
    28861 taaatcaggt taacggtcca catccacagc gtcgggtcca gcttaccttt accaccgttc
    28921 gatttcaggg aggtctggat caggaagaaa agtagataca gcagcatggc tggcagcatc
    28981 gacagtacgc gtccctgacg tgggttaacc acgctcagcg gtacgaccat aagtgccatc
    29041 ataaacacgg tgaataccaa cgtgatacgc cagttcagtt ctgcgcgggc acgatcggtg
    29101 tcagtgttcc acaatgtacg catgtccatc tggtcggtat cgttcgggtc gagcgccacc
    29161 gcctggtgac caatgatcgc ctgataatcc tggaaatccg taatgcggaa atcacgtaac
    29221 agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga cgacctggga gccgtcgcgc
    29281 agctgggtta aatgtccgga atcggccacc accacagaag gacgtgcatt accttttggt
    29341 cgaatttgcg cgaggaacac atctttgaaa tcgctgccgt caacgctttc gatgaacagc
    29401 accgaactgc cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc catgccaggg
    29461 ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg atgaccacgg tcccgcccac
    29521 atcacgttaa ccgccgcgac gattgccgtg aataccgcaa ggatcattgc cgctttcacc
    29581 agaaccgctt tgctcaggcc gcaggcatgc attaccgtaa tttcactttc ggtatacagt
    29641 ttgcccagcg tcatcagcag cccgaggaac aggcttaatg gcaggataag ctgcgccatt
    29701 tccggcacgc ccaacccgag aagggagagc accagattcg ccggaatatc gccgtcaacc
    29761 gctgcgccga ggatcctcac taacttttga cagaagaaga tcaaaagcaa gatgaagagt
    29821 atcgccagct ggcttttgag cgtctcccgc accagatatc ttatgattat cactttaaat
    29881 acgcccgtaa aaactcgtct tttgcaggat tttagcttgt ttcatggctt aaacgtcatt
    29941 tattctcttg agtcgtcgaa atcgtcgcta agataattat actcaacgga ttcacctctc
    30001 agattttgtt ctgacgtgcc aatgccgtaa taacgttaag attaacacga agtcatcgca
    30061 acagcggaca tgagttacga aagcttgcaa ttctatctgt agccaccgcc gttgtcttta
    30121 agattcagga gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg agaaacagcg
    30181 gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga ttgcagaaca
    30241 gctcgataaa atcagcgatg ggtacatcag cgccctgcta cgtcggggcg aactggaagg
    30301 aaaaccgggg cagacattgt tgctgcacca tgttccgaat gtactttccg agcgaattct
    30361 ccttattggt tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc aggttattca
    30421 gaaaaccatt aatacgctga atgatactgg ctcaatggaa gcggtctgct ttctgactga
    30481 actgcacgtt aaaggccgta acaactactg gaaagtgcgt caggctgtcg agacggcaaa
    30541 agagacgctc tacagtttcg atcagctgaa aacgaacaag agcgaaccgc gtcgtccgct
    30601 gcgtaagatg gtgttcaacg tgccgacccg ccgtgaactg accagcggtg agcgcgcgat
    30661 ccagcacggt ctggcgattg ccgccgggat taaagcagca aaagatctcg gcaatatgcc
    30721 gccgaatatc tgtaacgccg cttacctcgc ttcacaagcg cgccagctgg ctgacagcta
    30781 cagcaagaat gtcattaccc gcgttatcgg cgaacagcag atgaaagagc tggggatgca
    30841 ctcttatctg gcggtcggtc agggttcgca aaacgaatcg ctgatgtcgg tgattgagta
    30901 caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag gtttaacctt
    30961 cgactccggc ggtatctcga tcaagccttc agaaggcatg gatgagatga agtacgatat
    31021 gtgcggcgcg gcggcggttt acggcgtgat gcgtatggtc gcggagctac aactgccgat
    31081 taatgttatc ggcgtgctgg caggttgtga aaacatgcca ggcggtcgcg cctatcgtcc
    31141 gggcgatgtg ttaaccacca tgtctggtca aaccgttgaa gtgctcaaca ccgacgctga
    31201 aggccgcctg gtactgtgcg acgtgttaac ttacgttgag cgttttgagc cggaagcggt
    31261 gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc acattactgg
    31321 tctgatggcg aaccacaatc cgctggccca tgaactgatt gccgcgtctg aacaatccgg
    31381 tgaccgcgca tggcgcttac cgctgggtga cgagtatcag gaacaactgg agtccaattt
    31441 tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg attaccgcag gttgcttcct
    31501 gtcacgcttt acccgtaagt acaactgggc gcacctggat atcgccggta ccgcctggcg
    31561 ttctggtaaa gcgaaaggcg caaccggtcg tccggtagcg ttgctggcac agttcctgtt
    31621 aaaccgcgcc gggtttaacg gcgaagagta agtttgcgtt ttaccctcac cctaaccctc
    31681 tccctaaggg agagggaacc gatcgagcgc aatagaaatt cattgctcat cttttagggt
    31741 gaggaatgca gcaatattta aatccaccac aagaagcccc atttatgaaa aacgcgacgt
    31801 tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag caactggtgt
    31861 gtgaaattgc cgcagaacgt tggcgcagtg gtaagcgcgt actcatcgcc tgtgaagatg
    31921 aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa agctttgttc
    31981 cgcataattt agcgggagaa ggaccgcgcg gcggtgcgcc ggtggagatc gcctggccgc
    32041 aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc tttgcagatt
    32101 ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct ctgaaacaac
    32161 tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat acggcaacct
    32221 ggaaataatg gaaaagacat ataacccaca agatatcgaa cagccgcttt acgagcactg
    32281 ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt tctgcatcat
    32341 gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc agcaaaccat
    32401 catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt ggcaggtcgg
    32461 tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg ccgcagaaga
    32521 aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct gggaatggaa
    32581 agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact ccgtggactg
    32641 ggagcgtgaa cgcttcacca tggacgaagg cctgtccaat gcggtgaaag aagttttcgt
    32701 tcgtctgtat aaagaagacc tgatttaccg tggcaaacgc ctggtaaact gggacccgaa
    32761 actgcgcacc gctatctctg acctggaagt ggaaaaccgc gaatcgaaag gttcgatgtg
    32821 gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag attatctggt
    32881 ggttgcgact acccgtccgg aaaccctgct gggcgatact ggcgtagccg ttaacccgga
    32941 agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg ttaaccgtcg
    33001 tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct gcgtgaagat
    33061 caccccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc tgccgatgat
    33121 caacatcctg acctttgacg gcgatatccg tgaaagtgcc caggtgttcg ataccaaagg
    33181 taacgaatct gacgtttatt ccagcgagat ccccgcagaa ttccagaaac tggagcgttt
    33241 tgctgcacgt aaagcggtcg ttgctgcggt tgacgcactc ggcctgctgg aagaaattaa
    33301 accacatgac ctgaccgttc cttatggcga ccgtggtggc gtggtcatcg aaccgatgct
    33361 gaccgaccag tggtacgtgc gtgccgatgt cctggcgaaa ccggcagttg aagcggttga
    33421 gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct cctggatgcg
    33481 cgatattcag gactggtgta tctctcgtca gttgtggtgg ggtcaccgta tcccggcatg
    33541 gtatgacgaa gcgggtaacg tttatgttgg ccgcaacgaa gacgaagtgc gtaaagaaaa
    33601 taacctcggt gctgatgttg tcctgcgtca ggacgaagac gttctcgaca cctggttctc
    33661 ttctgcactg tggaccttct ctacccttgg ctggccggaa aacaccgacg ccctgcgtca
    33721 gttccaccca accagcgtga tggtatccgg cttcgacatc atcttcttct ggattgcccg
    33781 catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc aggtgccgtt
    33841 ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggccagaaga tgtccaaatc
    33901 caagggtaac gttatcgacc cgctggatat ggttgacggt atttcgctgc cagaactgct
    33961 ggaaaaacgt accggcaata tgatgcagcc gcagctggcg gacaaaatcc gtaagcgcac
    34021 cgagaaacag ttcccgaacg gcattgagcc gcacggtact gacgcgctgc gcttcaccct
    34081 ggcggcactg gcgtctaccg gtcgtgacat caactgggat atgaagcgcc tggaaggtta
    34141 ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga acacggaagg
    34201 tcaggattgc ggcttcaacg gtggtgaaat gacgctgtcg ctggcggacc gctggattct
    34261 ggcagagttc aaccagacca tcaaagcgta ccgcgaagcg ctggacagct tccgcttcga
    34321 tatcgctgca ggcattctgt atgagttcac ctggaaccag ttctgtgact ggtatctgga
    34381 gctgactaag ccggtaatga acggtggaac cgaagccgaa ctgcgcggta ctcgccatac
    34441 gctggtgacc gtactggaag gtctgctgcg cctcgcgcat ccgatcattc cgttcatcac
    34501 cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca ccatcatgct
    34561 gcagccgttc ccgcagtacg atgcatctca ggttgatgaa gccgcactgg ccgacaccga
    34621 atggctgaaa caggcgatcg ttgcggtacg taacatccgt gcagaaatga atatcgcgcc
    34681 gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac gtcgcgtaaa
    34741 tgaaaaccgt ggcttcctgc aaaccctggc gcgtctggaa agcatcaccg tgctgcctgc
    34801 cgatgacaaa ggtccggttt ccgttacgaa gatcatcgac ggcgcagagc tgctgatccc
    34861 gatggctggc ctcatcaaca aagaagatga gctggcgcgt ctggcgaaag aagtggcgaa
    34921 gatcgaaggc gaaatcagcc gtatcgagaa caagctggcg aatgaaggct ttgtcgcccg
    34981 cgcaccggaa gcggtcatcg cgaaagagcg tgagaagctg gaaggctatg cagaagcgaa
    35041 agcgaagctg attgaacagc aggctgttat cgccgcgctg taatcaaaac acagtaatga
    35101 aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg taaagaaggc
    35161 ataattcccc gtgaaatcct ggctaagaag cctttatctg gttgttcttc tttattgatt
    35221 aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca gtcaatcatc
    35281 catattttca gcagtggata agccagaccg cccgtaatcc cggatatcac caccaacgca
    35341 cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat acgcccatca
    35401 ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata actggtgcta
    35461 acagcaatcc caaaataata gattaactgc gcgattatca ttttacgttg catttccata
    35521 aattgctgta aattctcaat atcatcattt gcatacacag aactatcata cgcatttaat
    35581 atttgatcga agataatata accagcaaca gcgagaaaag gtaataaagc taaaaatgcg
    35641 agaatggcat attttataca atacgtagta tctaatttca ccttgaaacg atgtataccg
    35701 aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc attaaaaata
    35761 ccaatggaaa caataccaac tattgccatc agaaccacag atataataac actacttgaa
    35821 ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc aatggccatt
    35881 aaaatcggca agaaaaaggt tacccaccag aaccctttca tagagcaatt aaaactaaat
    35941 cttacgccgt tgagactggt catcaaggcc tgataacgca gtccttttgc tgccataaat
    36001 ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagtggcat atctgctgac
    36061 actgtcatta agattgcgaa atagcaaaaa acaaaaacaa gacaactaac aaaaacattc
    36121 ccaccagtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc atagagataa
    36181 cgcttacatt tcattaatgc ccatggcaaa tagataccta gcgttataat cgttaacaac
    36241 acattcacca cacaaataag aaaatatctc tcacctgtac catgaaaaac aaacgaatgg
    36301 gacggaacat ccatttcatt aataacttga gccataaaaa
//
LOCUS       DAPGWD010000032        34455 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPGWD010000032 DAPGWD010000000
VERSION     DAPGWD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.032"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..294)
                     /locus_tag="RHX88_001535"
     CDS             complement(<1..294)
                     /locus_tag="RHX88_001535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230235.1"
                     /translation="MSNNNSQKSTINVIGSNNDAKVNQYNISIYANDVLSIPNEIREG
                     IVDALENELVHSELNIFLCYPNKLQDKHFDSCIKNLKTELIKIDAVVYEGGGKQ"
     gene            complement(284..490)
                     /locus_tag="RHX88_001536"
     CDS             complement(284..490)
                     /locus_tag="RHX88_001536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230236.1"
                     /translation="MQEKLQWVLNNKEWLFSGAGLLIVTFIIFIAKLFFRSEKDGNKS
                     NKQKSCMFTIGSNNKAKNTQKNEQ"
     gene            complement(490..1665)
                     /locus_tag="RHX88_001537"
     CDS             complement(490..1665)
                     /locus_tag="RHX88_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2230237.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAEAREKRDTARKQVANGIDPVEERKAQKLAQQLSTENSFEA
                     ICREWHSSKADRWTLAYREEIIKTFEQDVFPLIGKRPISEIKPLELLEVLRRIEKRGA
                     LEKTRKVRQRCGEVYRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFIRD
                     LEAYTGSIITKNATKIVMLTGVRTQEMRFATWEEVDLDKGIWEIPAERMKMRRPHIVP
                     LSTQVVDLFKQLKPITGHYPYIFIGRNNRSKPISKESVSQVIELLGYKGRATGHGFRH
                     TMSTILHEQGFDSAWIELQLAHVDKNSIRGVYNHANYERNRKKMLQWYADYILTGDV"
     gene            complement(1828..1922)
                     /locus_tag="RHX88_001538"
     tRNA            complement(1828..1922)
                     /locus_tag="RHX88_001538"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1886..1888),aa:Sec,seq:tca)
     gene            2215..3597
                     /locus_tag="RHX88_001539"
     CDS             2215..3597
                     /locus_tag="RHX88_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDV2230238.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3607..5925
                     /gene="yicI"
                     /locus_tag="RHX88_001540"
     CDS             3607..5925
                     /gene="yicI"
                     /locus_tag="RHX88_001540"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDV2230239.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFAGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5978..7687)
                     /locus_tag="RHX88_001541"
     CDS             complement(5978..7687)
                     /locus_tag="RHX88_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV2230240.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(7808..9199)
                     /gene="xanP"
                     /locus_tag="RHX88_001542"
     CDS             complement(7808..9199)
                     /gene="xanP"
                     /locus_tag="RHX88_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDV2230241.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9479..10684
                     /gene="gltS"
                     /locus_tag="RHX88_001543"
     CDS             9479..10684
                     /gene="gltS"
                     /locus_tag="RHX88_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV2230242.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10687..11553
                     /locus_tag="RHX88_001544"
     CDS             10687..11553
                     /locus_tag="RHX88_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230243.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11538..13619)
                     /gene="recG"
                     /locus_tag="RHX88_001545"
     CDS             complement(11538..13619)
                     /gene="recG"
                     /locus_tag="RHX88_001545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDV2230244.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDVLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELQLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKMAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13625..14314)
                     /gene="trmH"
                     /locus_tag="RHX88_001546"
     CDS             complement(13625..14314)
                     /gene="trmH"
                     /locus_tag="RHX88_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDV2230245.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(14321..16429)
                     /gene="spoT"
                     /locus_tag="RHX88_001547"
     CDS             complement(14321..16429)
                     /gene="spoT"
                     /locus_tag="RHX88_001547"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDV2230246.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16448..16723)
                     /gene="rpoZ"
                     /locus_tag="RHX88_001548"
     CDS             complement(16448..16723)
                     /gene="rpoZ"
                     /locus_tag="RHX88_001548"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDV2230247.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16778..17401)
                     /gene="gmk"
                     /locus_tag="RHX88_001549"
     CDS             complement(16778..17401)
                     /gene="gmk"
                     /locus_tag="RHX88_001549"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDV2230248.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17659..19341
                     /gene="ligB"
                     /locus_tag="RHX88_001550"
     CDS             17659..19341
                     /gene="ligB"
                     /locus_tag="RHX88_001550"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDV2230249.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(19338..19955)
                     /locus_tag="RHX88_001551"
     CDS             complement(19338..19955)
                     /locus_tag="RHX88_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDV2230250.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(20247..21071)
                     /gene="dinD"
                     /locus_tag="RHX88_001552"
     CDS             complement(20247..21071)
                     /gene="dinD"
                     /locus_tag="RHX88_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDV2230251.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(21292..22155)
                     /locus_tag="RHX88_001553"
     CDS             complement(21292..22155)
                     /locus_tag="RHX88_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDV2230252.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            22282..22998
                     /gene="rph"
                     /locus_tag="RHX88_001554"
     CDS             22282..22998
                     /gene="rph"
                     /locus_tag="RHX88_001554"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDV2230253.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            23064..23705
                     /gene="pyrE"
                     /locus_tag="RHX88_001555"
     CDS             23064..23705
                     /gene="pyrE"
                     /locus_tag="RHX88_001555"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDV2230254.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(23742..24338)
                     /gene="slmA"
                     /locus_tag="RHX88_001556"
     CDS             complement(23742..24338)
                     /gene="slmA"
                     /locus_tag="RHX88_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDV2230255.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(24445..24903)
                     /gene="dut"
                     /locus_tag="RHX88_001557"
     CDS             complement(24445..24903)
                     /gene="dut"
                     /locus_tag="RHX88_001557"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDV2230256.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24881..26101)
                     /gene="coaBC"
                     /locus_tag="RHX88_001558"
     CDS             complement(24881..26101)
                     /gene="coaBC"
                     /locus_tag="RHX88_001558"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDV2230257.1"
                     /translation="MSLAGKKIVLGVSGGIAAFKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARIAAGM
                     ANDLVSTICLATPALVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            26273..26941
                     /gene="radC"
                     /locus_tag="RHX88_001559"
     CDS             26273..26941
                     /gene="radC"
                     /locus_tag="RHX88_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV2230258.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            27158..27394
                     /gene="rpmB"
                     /locus_tag="RHX88_001560"
     CDS             27158..27394
                     /gene="rpmB"
                     /locus_tag="RHX88_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDV2230259.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            27415..27582
                     /gene="rpmG"
                     /locus_tag="RHX88_001561"
     CDS             27415..27582
                     /gene="rpmG"
                     /locus_tag="RHX88_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDV2230260.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            27680..28489
                     /gene="mutM"
                     /locus_tag="RHX88_001562"
     CDS             27680..28489
                     /gene="mutM"
                     /locus_tag="RHX88_001562"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDV2230261.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(28528..29007)
                     /gene="coaD"
                     /locus_tag="RHX88_001563"
     CDS             complement(28528..29007)
                     /gene="coaD"
                     /locus_tag="RHX88_001563"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDV2230262.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(29015..30292)
                     /gene="waaA"
                     /locus_tag="RHX88_001564"
     CDS             complement(29015..30292)
                     /gene="waaA"
                     /locus_tag="RHX88_001564"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDV2230263.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            30705..31763
                     /gene="rfaQ"
                     /locus_tag="RHX88_001565"
     CDS             30705..31763
                     /gene="rfaQ"
                     /locus_tag="RHX88_001565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDV2230264.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31760..32884
                     /locus_tag="RHX88_001566"
     CDS             31760..32884
                     /locus_tag="RHX88_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV2230265.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            32877..33674
                     /gene="rfaP"
                     /locus_tag="RHX88_001567"
     CDS             32877..33674
                     /gene="rfaP"
                     /locus_tag="RHX88_001567"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDV2230266.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKLSVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            33690..>34455
                     /locus_tag="RHX88_001568"
     CDS             33690..>34455
                     /locus_tag="RHX88_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HDV2230267.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVC"
BASE COUNT         8190 a         8467 c         9082 g         8716 t
ORIGIN      
        1 ctgtttccct cctccctcat ataccacagc atctatttta ataagttcgg tctttaagtt
       61 tttaatacat gagtcgaaat gcttgtcttg taatttattg gggtagcata aaaaaatatt
      121 aagctcagaa tgaactagtt cattctctaa agcatcaact attccctctc taatttcgtt
      181 agggattgat agaacatcat tggcgtaaat tgaaatattg tattgattaa ccttggcatc
      241 attatttgag ccaataacgt ttattgttga tttttgagag ttattattgc tcattcttct
      301 gagtattttt tgctttattg ttcgacccaa ttgtaaacat gcaagacttt tgtttgttag
      361 atttgttccc atctttttcc gaccgaaaga ataatttagc gataaatatg ataaatgtga
      421 caatcaataa gccggcacct gagaataacc attctttatt attcaatacc cattgaagtt
      481 tctcttgcat cagacatctc cagttaaaat ataatcagca taccattgta acattttttt
      541 tctatttcgc tcataattag catgattgta cacaccgcga atactattct tatcgacgtg
      601 cgctagctgc aattcaatcc aagcactatc aaacccttgt tcatgcaata ttgtcgacat
      661 tgtatgtcta aaaccgtgac ccgtagcacg tcctttgtag ccaagtagct caatcacttg
      721 tgacacactt tctttagaaa taggcttgct tcgattgttt ctaccaataa aaatgtaggg
      781 ataatgacca gtgataggct tgagctgttt aaagaggtct accacctgag tagacaaagg
      841 tacaatatgt ggtctgcgca ttttcatccg ttctgctggt atctcccata tacctttatc
      901 caagtcaact tcttcccatg tagcaaagcg catctcctgc gttcttacac cagttagcat
      961 gactatcttt gtagcatttt tggtgatgat gctgccggtg tatgcttcca aatcccgaat
     1021 gaaatgaggc agctcttcgg cagatagaaa aggatggtgc ttttgcttcg gaacggccag
     1081 agcgatggct aaatcaggcg caggattgta ttcagcgcgg ccagttatga ttgcatagcg
     1141 ataaacctca ccgcacctct gacgcacctt acgtgttttc tcaagtgccc cacgcttctc
     1201 tattcggcgc aatacttcaa gcagttctaa cggtttgatt tcactgatag gacgtttccc
     1261 aatcaacggg aacacatctt gctcaaatgt tttaatgatt tcttcgcggt aggccagtgt
     1321 ccagcgatcg gctttactag aatgccactc ccgacatatc gcttcgaatg agttttctgt
     1381 tgagagctgc tgtgcaagct tttgggcttt acgttcttcc actgggtcta tgccgtttgc
     1441 tacctgctta cgagctgtat cgcgtttctc acgtgcttct gcgaggctca caaggtcgta
     1501 gctgccaaat gacattagcc gtgctttccc ggcaaagcgg aaacggaaac gccagccttt
     1561 cgagccatcg ggattgataa gcaatgacag gccttgcccg tcgttcaatg tgtatggctt
     1621 gtcttggggc tttgctcgtt tgatttgtat gtctgtaagt gccatgtgta taagccaaaa
     1681 atgtgtataa aaaatctata cacatcacta tacatatttt tcatggattc agggagaact
     1741 tgtcggactg ctacggatga ttaatctaca atggtgattg aaaataaagg gtttttaaga
     1801 ctttctcgga tgggtgagga ggaactttgg cggaagatca caggagtcga acctgcccgg
     1861 gaccgctggc ggccccaact ggatttgaag tccagccgcc tcaccggaga cgacgatctt
     1921 ccgcgcctcg attgctacat ggaggcgggg cgcattatag ctacttcctt gagtttctac
     1981 atcccccaga tcagtttttt ccgcctaata cacgcagcgt attatcacga aagcattata
     2041 gttcatataa aacccatatt ttacaatgat atatgagcgg aattaacgct accacgatca
     2101 cataaaacaa gaatcataaa ttaataacca gatatcggaa tattcgctct cacagggatg
     2161 gtttacaaaa tgcgttatcg gtctcagcac ccctatagca tcaaggaaaa gcagatgaag
     2221 agtgaagtgt tgtccgttaa agagaaaatt ggttatggca tgggagacgc cgccagccac
     2281 attattttcg ataacgtaat gttatatatg atgttctttt ataccgatat ttttggcatt
     2341 cctgccggtt ttgtcggaac catgtttttg gtcgctcgtg cactggatgc gatttccgat
     2401 ccttgcatgg ggttgttggc cgatcgaacg cgctctcgct ggggtaaatt tcgtccgtgg
     2461 gtactgtttg gcgcactgcc attcgggatc gtctgtgtac tggcctatag cacgccagat
     2521 ctcagtatga acggcaaaat gatctatgca gcaattactt acaccctact taccttactt
     2581 tataccgtcg tcaatatccc ttactgcgca ttgggtggtg taatcaccaa tgacccgact
     2641 cagcgtatct cgctgcaatc ctggcgtttt gtgctggcga ccgcgggagg catgctttct
     2701 actgttctga tgatgccact ggttaattta attggcggtg ataataaacc actcggtttc
     2761 cagggcggta tcgcggtcct ttccgtggtg gcattcatga tgctggcatt ttgtttcttc
     2821 accactaaag aacgcgttga agcaccacct acaacaacgt ctatgcggga agatttacgt
     2881 gatatctggc aaaacgacca gtggcggatt gtcggtttac taaccatttt caatatcctg
     2941 gcggtgtgcg tacgcggtgg ggcgatgatg tattacgtca catggatttt gggcacgccg
     3001 gaagtgtttg tcgcttttct caccacttat tgcgtgggta acctgattgg ttccgcactg
     3061 gcaaaacctc tgaccgactg gaaatgtaaa gtcactatct tctggtggac gaacgccctg
     3121 ctggcagtga ttagcctcgc gatgttcttt gttcccatgc aggccagcat cactatgttt
     3181 gtcttcatct tcgtgattgg tgtgttgcat caactggtga cacctatcca gtgggtaatg
     3241 atgtccgata ccgtcgacta cggcgagtgg tgcaatggta aacgcctgac cgggatcagt
     3301 tttgctggca cgctgtttgt gctcaaactg gggttggcct tcggcggcgc tcttatcggc
     3361 tggatgctgg cttatggcgg atatgatgcg gcagaaaaag cgcagaacag cgccacgatt
     3421 agcatcatta ttgcgctatt cacgattgtt ccggcgatct gttatttgct gagcgcgatt
     3481 atcgctaaac gctactactc actcacgacg cacaatctga aaaccgttat ggaacagctg
     3541 gctcagggta aacgccgttg ccagcaacaa ttcacctctc aagaagtgca gaactaagga
     3601 acggcaatga aaattagcga tggaaactgg ttgattcaac ctggcctcaa tttgattcac
     3661 ccgcttcagg tgttcgaggt tgaacagcag gataatgaaa tggtggtcta tgctgccccc
     3721 cgtgatgtgc gtgaacgtac ctggcagctt gatactcctt tatttacgtt gcgctttttc
     3781 tccccacagg aaggtattgt cggtgtgcgg attgagcatt ttcagggggc gctgaataac
     3841 ggtcctcatt atccgctcaa tattttgcag gacgtgaagg tcacaatcga aaacacagaa
     3901 cgttatgctg agtttaaaag tggcaactta agcgcgcgtg tcagcaaagg tgagttctgg
     3961 tcactggatt ttctgcgcaa cggcgaacgt attaccggta gtcaggtgaa aaataatggc
     4021 tacgtgcagg acacgaataa tcaacgcaat tatatgtttg agcggcttga tcttggcgtt
     4081 ggcgaaacag tttacggtct gggagagcgc tttactgccc tggtgcgcaa tggccagacg
     4141 gtagagacct ggaaccggga cggcggcaca agtactgaac aggcgtataa aaatatcccg
     4201 ttctacatga ctaaccgtgg ttatggggta ctggtcaatc atccccagtg tgtctctttt
     4261 gaagtgggat cggagaaagt ctccaaagtg cagttcagcg ttgagagtga atatctcgaa
     4321 tactttgtta tcgacggccc gacgccgaaa gcggtacttg atcgttatac ccgctttgct
     4381 ggtcgtccgg cgctgccgcc cgcgtggtcc ttcggcctgt ggctaaccac ttcatttacc
     4441 accaactacg acgaagcgac ggtaaacagc tttatcgatg gtatggcgga acgcaatctg
     4501 ccgctgcatg ttttccactt tgactgtttc tggatgaaag ccttccagtg gtgcgatttt
     4561 gagtgggacc cgctgacttt ccctgacccg gaagggatga tccgccgcct gaaagcgaaa
     4621 ggactgaaaa tctgcgtctg gattaacccc tatatcggtc aaaaatcccc cgtctttaaa
     4681 gagttacaag agaaaggcta tttactcaaa cgcccggacg gttcgctatg gcagtgggat
     4741 aaatggcagc caggtctggc gatttatgac tttaccaatc cggatgcctg caaatggtac
     4801 gccgacaaac tgaaaggtct ggtcgcgatg ggcgttgatt gctttaagac cgactttggc
     4861 gaacgtatcc caactgatgt tcagtggttt gacggttccg atccgcagaa aatgcataac
     4921 cattatgcgt acatctacaa cgaactggtg tggaacgtgc tcaaggacac cgttggtgag
     4981 gaagaagctg tcttgtttgc ccgctcggcc tccgtcggtg cgcagaaatt cccggtacac
     5041 tggggtggcg attgttacgc taactacgaa tcaatggcgg aaagcctgcg cggtggtttg
     5101 tctattggcc tttcaggttt tggcttctgg agccacgata tcggcggctt tgaaaatacc
     5161 gctccggcgc acgtttacaa acgctggtgc gcgtttggtt tgctctccag ccatagccgt
     5221 ttacacggta gcaaatctta tcgtgtgccg tgggcctacg atgatgagtc ctgtgatgtg
     5281 gtgcgcttct tcacgcaact gaaatgccgc atgatgccgt atctgtatcg tgaagctgcg
     5341 cgtgcgaacg cgcggggtac gccgatgatg cgggccatga tgatggagtt cccggacgat
     5401 ccggcttgtg attaccttga ccgtcaatac atgttaggcg acaacgtgat ggttgcgccg
     5461 gtgttcactg aagcgggcga tgtgcagttc tacctgccgg aaggtcgctg gacacacctg
     5521 tggcacaacg atgagctcga cggtagtcgc tggcataaac agcagcacag cttcctgagt
     5581 ctgcccattt atgtgcgtga taacacccta ctggcgctgg gcaacaacga tcaacgtccc
     5641 gattacgtgt ggcacgaagg cacggcattc cacctcttta atctgcaaga cgggcatgaa
     5701 gccgtctgtg aagtgcccgc tactgacgga tcggtgatct ttactttaaa agcagcacgt
     5761 actggcaaca cgattactgt gactggtgcg ggcgaggcga agaactggac gctgtgcctg
     5821 cgcaatgttg tgaaagtaaa tggtctgcaa gacggttcgc aggctgaaag tgagcagggg
     5881 ctggtggtga agcctcaagg gaatgcgctg acaattacgt tgtaatctat tgatggaatg
     5941 ccgggtacgt ctgagtcgta cccggcgaga ttgatgttta ccgcaccggc tccgtcgatg
     6001 taacttcacc gctggaaacg atgccagcat tcatggtttg agtcacttgc tgctttgcgg
     6061 cgttcacggc atgcaattgc ccgctaaccg taggtttcaa cgggacattg gcctgaatat
     6121 cgccactggc ggtaagctga atattgccgt cgccagtcaa cggtaattta ggccatcccc
     6181 actgttgcaa aatattcacc ggcacaccgc gtccattcag gctgatatgt gtctgacgtt
     6241 gtggcgtttg tgaaacactg gcggtggctt ccagaatgcc tttttcagta aatgcactca
     6301 gttcgctgat attcaccgtg ctgctgttgg cggtcagcgc cagcgacggg cgacgaacat
     6361 caacacgatt gaatgtcgcg gcggcggcat tcagattcgc cgagccactc cagacgcccc
     6421 atttatgatc ggtaaccagc gtcaggttgg caccgtaacc atcgagcgcg gtgagctgcc
     6481 acgggaagtc agggtcgata tcaatgatca gattgcggct ggcgctaaat ctcttcagtt
     6541 gcaggctgtt taaccaaccg ggtgtcgttt ccatccacaa ctgttgccag tttttcggca
     6601 aggtatattc cagcccggca attgccacat catcaaggat caacgttttc ccgtcacgca
     6661 gccagttccc tgacgttctg accatacccc cttcccagcg gctggtgaac tggcgcagcg
     6721 ctacgccctg cggggaaaat tccgcgttta taatcgggtc aaataaatgc agcgaaccat
     6781 aaatgaactc gctggcgttc atcgacagtt tgccttcctg tgtctgccag tcatctttac
     6841 tgaaggtcat gttgcgcaag ctgagatcga ggtcggtcac cgcccagtcc ggaccttgca
     6901 gacgagcatc gatgacttcc aggcgaccaa tctgcaacga cgggacagag cgtaatggcg
     6961 caaagaagtc ggttagcgat ttttcgcttt gtagacggat atccgccatg cgcagatttt
     7021 ccacttgcca gctgccgtcg gcgttacgct gcgcgtttcc ggttaatgtc ccgcgggcga
     7081 tgtcggcacc caggttagtc agcgtaacgc gatcgttatc aatactgcct tcaatcagta
     7141 cattggtggc aggaacatca ttgagcgaaa gcgatccggc actaaactga atctgcgcct
     7201 tcgtacccag cactttaccg gcttctggtg accacggaac cacgccgcca tttacccgct
     7261 gcgcgctcag tttccattcg ctattcgggc tattaaacgc catatcacgc agttgcagac
     7321 gatcggcttt gaacggtagc ggcgcggtct ggtcggtgag attcagcgtc ccgttttcca
     7381 gcaggatggt atcgacatgg cgtggttcgg tcagttgccg actgcttagc gcaatgtcga
     7441 cactttttgc caccagggtc gcgggctgac catcacgacc aaacgtgacg ttctccagca
     7501 cgatatgaga tggcgcggaa aaacggtgat ccatcgcccc gaaggccaga tgatagtcgc
     7561 tattctcgga aacccatgcg ctgatatgtt ctgctcccca gcgggtttgc agaagaaaat
     7621 agaggccagc gatcaccact aacagagcga tgagaatgta gagaagcagc ttcccaataa
     7681 atttcatggt cttccatccc gtgaaatgca cataagggag ttatgcacga tttacgcgca
     7741 atcctcaagg cgggaatggt gaaagaggtg agactgcggc ggtaattatc ttaccgccgc
     7801 cagtgaatta ctgtttttct ggtgggaaaa tcagattcag cacgatggca gtaataccgc
     7861 ccgcggcgat cccggaggag agcaggtttt tcagccattc aggggcaaac tgcaaaatca
     7921 gcggctgctg agacacgccc agaccaaccg ccagcgacag cgcgataatc agaatcgccc
     7981 gacggttcag cggctcacga gaaacgatac gcacaccgga ggcggcgatg gtgccaaaca
     8041 ttacaagcgt tgcgccgccc agaaccggtt ctggaatgtg ttgtacaaaa ccgctcactg
     8101 ccgggaacag acccagcacg atcaacatca gcgcgacgac aaaaccgaca tagcggctgg
     8161 caacaccagt caactggatc actccgttgt tttgcccgaa gcaggagttc gggaaggtgt
     8221 taaacaccgc cgaaacaaac gagttcaggc cgtttgccag cacgccgcct ttcaggcgtt
     8281 tcatgtacag cggaccggac actggctgtt cggaaacgtc agaggtcgcc gtgatatcgc
     8341 caatggtttc cagcgaagtg atcataaaga ccagcatcag cggcagcagc agactccatt
     8401 caatgccaag accgtaatag agcggcgttg gcaccataat cagttcttgc gtcatcggtt
     8461 cgttgctttc tggcaacatg cccataaacc acgccagcgc atatccggcc gccatcgcaa
     8521 tcaccagtga ggccacgcgt aagtaagggt tacgttgacg gttaagcagg ataattaagg
     8581 ctaagaccac gcctgccagc agcagatttt tcggtgcgcc gaaggtgtta tcgctcatgg
     8641 ctgcgtaacc gccgccaatg gacgttaacc caacctgaat tagcgacagg ccgataatca
     8701 tcaccacaac gccagaaacc agcggcgtaa taatgcggcg cgccagatgc agaacgcggg
     8761 agatcaccat ctcggtgcaa cttgccagca tcaacgtgcc gaacaaagcc gccatcatgg
     8821 taggaacatc agcaccaccg gttttcagcg cggtaccgcc cataatcagc ggggcaacaa
     8881 agttgaagct ggtgccctga atagacaaca gcccggagcc aaccggaccc caggccttaa
     8941 tttgaataat cgatgccaca ccggaggcaa acagcgacat actaataatg tgttgcgtgt
     9001 cttgtgccgg taaacccagc gcctggcaga ttaatagcgc tggcgtgatc accgcaacga
     9061 acatcgccag cagatgctga caggcggcaa acagggtttg aggaagcggc ggacgatctt
     9121 caagacggta aatcagttcg ctgttttgag tctgcgcaac cggttgcgca ttttctgact
     9181 cgagggtgga aacagacatc gaaaacaatc ccgtggtagc aaagcgggca ttttagctga
     9241 ccgaaagaga aaagcaaacg tttgccacat cctcatctaa atatttaaat gagataaatg
     9301 cactttttta tataactttt ggtatttttc tgcttaaaat ccatgccatt tgttcattaa
     9361 cagctgagaa tttgacccag gaaagcataa ctcgataccc gtcatagttc atgaagttgc
     9421 cactgcgcga tgaagtatga cgagtatgaa agagtgatgc ggatacaaag gagtaactat
     9481 gtttcatctc gatactttag caacgcttgt tgccgcaacg ctgacgttgc tgctcgggcg
     9541 taagttggtc cattccgtct cctttttgaa gaaatacacc ataccggaac ctgttgcggg
     9601 tggtttgttg gtggcgctgg cgctactagt actgaaaaaa agcatgggct gggaagtcaa
     9661 ctttgatatg tccctgcgcg atccgttaat gctggctttc ttcgccacca ttggcctgaa
     9721 cgccaacatt gccagtttgc gtgccggtgg gcgtgtggtt ggcatcttct tgattgtggt
     9781 tgttggtctg ttggtgatgc aaaatgccat tggcattggt atggctagcc tgttagggct
     9841 tgatccgctg atggggctgt tggccggttc tattacgctt tccggcggtc acggtacggg
     9901 cgctgcgtgg agtaaattgt tcattgaacg ttatggcttc accaatgcga cggaagtggc
     9961 gatggcctgt gcaacgttcg gtctggtgct gggcggcttg attggcggtc cggtggcgcg
    10021 ctatctggtg aaacactcca ccacgccgaa cggtattccg gatgaccagg aagtcccgac
    10081 ggcgtttgaa aagccggatg tgggacgcat gatcacctcg ctggtgctga ttgaaactat
    10141 cgcgctgatt gctatctgcc tgacggtggg gaaaattgtt gcgcaacttt tggctggcac
    10201 tgcttttgaa ctgccgacct tcgtctgtgt actgtttgtt ggcgtgattc tgagcaacgg
    10261 tctgtcaatg atgggctttt accgcgtctt tgagcgtgcg gtatccgtgc tgggtaacgt
    10321 aagcctgtcg ttgttcctgg cgatggcgct gatggggctg aaactgtggg agctggcttc
    10381 gctggcgctg ccgatgctgg cgattctggt ggtacagacc atcttcatgg cgttgtatgc
    10441 catcttcgtt acctggcgca tgatgggcaa aaactacgat gcggcagtgc tggctgcggg
    10501 tcactgtggt tttggcctcg gtgcaacgcc aacggcaatc gccaacatgc aggcgatcac
    10561 tgaacgcttt ggcccgtcgc acatggcgtt tttggtggtg ccgatggtcg gtgcgttctt
    10621 tatcgatatc gtcaatgcgc tggtgattaa gctgtattta atgttgccga tttttgccgg
    10681 ttaaaaatga aaaagcgtcg gaaggaaccg gaaacgctac gtgaacactg tcgccatatt
    10741 tttggtgatg agccgccagt gctctgtgta tgggaaacgg aatttgatta cgcagatgcc
    10801 gaactcaagg cgctggcggc taaagagtgg cagcagatca gcgaatggga tttgagtgct
    10861 tactatgtac tcaatctcgt ttataacgaa ccgatgcaaa tagagttgtt tcgctatctg
    10921 tttccgctct gcctggcgca atggcatgaa accgttttag ctggcggtta tggtgatcac
    10981 ttcgaagaat ccttaatgaa ggcattgtgt cggccctatc tctggcagga gatgatgaat
    11041 gcttcccaac gccagcaggt acggcaattt ttactggaca ccgcgttgca gcgtatggat
    11101 aacgagcgtg gtttcaataa cgttctttgc tggctggcgg tgttcaatac tctgggtggg
    11161 gccgcgccgt taattcgttc tctctggtcg cgctggtggg cgctggatac gccaggaaaa
    11221 gcggtgtgtg ctattcagta tgccgcgcat ctaatttatc ccatagaagc caatccgtta
    11281 tggtcgcaag aatggatagg ctgggggcat ccattaggcc ataaagatgg ctggtcctct
    11341 gataatcgcg cttttcttcg ccaaatgtta acacctgaaa tgattgtggc tggcgtacag
    11401 gcagcggctg aaattctgcg tggtgaaccg gaaggcgcaa tggcggcgcg aattgcacag
    11461 gatgcgtatg aggcaatgga tatcctcact atccagattg aagatttact gcgtgacttg
    11521 tcttgtgatg agtctggtca tgcgttggag taacgttccg tctccggcat ccagcgttct
    11581 atcagggctt ttgcttgttg tgggtaacgt tcgtgaatat gtcgcgccag gcgctgaact
    11641 tccgggatca tcgcctgatc gcgcagtaaa tccgccactt taaattcagc attacccgtc
    11701 tgacgcgtgc ctaacaattc gccagggccg cgaatctcca gatctttttg cgcaatcaca
    11761 aaaccgtcgt tactgtcgcg cagcacttgc aggcgaattt gcgccatttt ggagagcggc
    11821 gttttgtaga gcagcacgca gtgagaagcc accgcgccac gacctacgcg cccgcgcagc
    11881 tggtgtaact gcgctagacc cagacgctca gggttttcga taatcatcag actggcgtta
    11941 ggcacatcaa cgccgacttc aataacggta gtggcaacca gcagttgtag ctcaccttgt
    12001 ttaaacgacg ccatcaccgc ctgtttctcg gctggtttca tccgcccgtg taccaggcca
    12061 acattcaatt ctggtagcgc cagtttcaac tcttcccagg tagcttccgc cgcctgcgct
    12121 tccagtaact ctgactcttc aatcaacgta caaacccagt acgcctgacg accttcagtc
    12181 atgcaggcgt ggcgcacgcg gtcaatgatg tcggtacggc gggtatcagg aatagcgacc
    12241 gtagtcactg gcgtgcggcc tggcggcagc tcatctatca ccgaggtatc gagatcggca
    12301 tacgccgtca ttgccagcgt gcgggggatc ggcgtggcgg tcatgatcaa ctgatgcgga
    12361 tggaagccct gctgctggcc tttctcccac aatgccagac gctgatgcac gccaaaacga
    12421 tgctgttcgt cgataatcac cagcgccaga ccgttaaact gcacctgttc ctggaagatg
    12481 gcgtgtgtac cgacaatcat ctgcacctga ccgctggcga tggcttcctg ctgtgccagt
    12541 cgcgctttac ctttctgctt accggcgagc cagcccactt cgataccgag cggtgcaaac
    12601 cagttgcgga agttattggc gtgctgctcg gcgagtaatt cggttggtgc catcagggcc
    12661 acctgtttgc cgtgggcaat cgcacgcagc gcggcgaggg cggcgaccag tgttttaccg
    12721 gaacctacat cgccctgcac caggcgcatc attggaacat ccagcgccat atcatgctcg
    12781 atctccgcca ctacgcgtgc ctgtgcgccc gttggcttga acggtaaggc ggcgaggagt
    12841 ttatttttca gcacgtcatt ggcgctcagc ggctgggcat gaaaacgctg tgctccggca
    12901 cgtaaggcta acatgctgag gttgtgcgcc agcagttctt ccagaatcag acgacgttgc
    12961 gccggatgct gcccggtttc cagatcgcta agctgtagcg tcggcggcgg gcggtgcaaa
    13021 gtgcgcaacg cttccggtag cgtcatcatt ccttgtgaca gttccggcgg caggagttct
    13081 tcaatggcgc aggtgtcgag cagatccagc gcctggtcgg ttaatttacg cagcgtggcc
    13141 tgctttacgc cttccgttgt tggataaacc ggcgtgagcg tttcctgtaa ttctggcgtg
    13201 ctgagatcgc cctgcacgcg gtattccggg tggatcatct ccgcaccata tttaccgcgc
    13261 tttgcttcgc cataagccag tacacggcgg cccgtcgcca ggctattttt cattgccgcg
    13321 ctgaaattga aaaagcgcat ggtgaggatg ccggaaccgt cgctgatctg gcaggtcatc
    13381 atccgccgac cgccgaagga gatattgcag ttcagcactt cgccttccac cgtggcataa
    13441 acgcccggca gtagttctcc gatggggtag agatgggtgc gatcttcgta gcgcagggga
    13501 aggtgtaaga gtagatcctg cacggtatgc aggttgattt tcgccagttt gttactaagt
    13561 gctgcgccaa cgcccgttag ggaactgagt gggacagcat ctaacaggcg acctttcatg
    13621 gcacttaccc tgcagcctgc atagtagccc accagtcggc atcagcttcg atctcgcctt
    13681 gctgattgac gtggggataa ggcaggcctt tgcgttttgc gactttcgcc agcaccggat
    13741 agccgccttc aaacaacagg cgttgttgct ctgcttccgg caacatgctg ttttcacgca
    13801 ggtacatgcc tgcattttgc cgctgacgct gggcttcgta aagaatgagg gctgaggcaa
    13861 cggaaacatt cagcgactgc accatgccga tcatcggaat gatgatgtcc tgatccgcca
    13921 gggccaatgc ttcctgcgtg atgcccgttt tctcctgtcc catcaaaatg caggtcgggc
    13981 gagtgtaatc aatttcgcgg aaatcgacag cgttatcaga aagatgggtt gccagaatct
    14041 gcatgccctg gcctttgaga tgagcgacgg catcgccaat ggtgcggtgt gttttcacct
    14101 gtacccagct gttactaccc gccgctgccg aagccatggt gcgcatgcgg ctaccaggcc
    14161 agacggcgtg aacttcatgt acgccaacgg catctgcggt acgaataatc gcagaaacgt
    14221 tatgaggttt gtggacctgc tccatgcaga cggtcagatc aggctgccgc ctggcgagca
    14281 tttcgcagat gcgtgcataa cgtgttgggt tcataaaaca ttaatttcgg tttcgggtga
    14341 ctttaatcac gtctggcatc acgcggattt tgcgcatgat attcgccaga tgcacacggt
    14401 cacgggcggt cagacgaata aaggcgctgt agacgcgacc atctttctct tccgtattca
    14461 aactttgaat attcgaagtc gtggtgttaa ttgccgccgt caggtttgcc agcgcgccct
    14521 gatgattgaa catctccacc ttgatttcgg tgatgaactc ctgcgccgtc tctttatccc
    14581 attccacagc cataaacttc tctggctctt tctggtagcc acggatatta cggcaggatt
    14641 catggtggat caccagacct ttaccggggc tgacgtgggc gataatcggg tcgccaggaa
    14701 tagggcggca gcatttcgca aaggtgatca gcacgccatc ggcaccttta atgggcagat
    14761 gtccgtggct ttgggttgcc ggtggaatgg aggcgtcccc atgttgcaga tttttcgcga
    14821 ccaccacgct cattgcgtta ccaagtccga tttctgccag cagatcgtca agcgttgcca
    14881 gcttcatgcg atccagctcg cgctgaatat tttcctgcgg gatttcattc agcttacggc
    14941 taccacccaa agcatggttg agcagacgac ggcccaggct tacagaatca tcacgcttga
    15001 ggtttttcag caactgacga attttggcgc gcgcttttga gctaacgaca aagttcagcc
    15061 acgcggcatt cgggcgagcg cccggtgcgg taatgatttc aacggtttga ccgctggtaa
    15121 gcggctgcga cagcgggtaa ggctggcggt caacgcgtgc gcccacgcag gcatgaccga
    15181 tatcggtatg cactgcataa gcgaagtcga cgggcgttgc accggcaggc agctcgacaa
    15241 ttcgcccttc cggtgtgaaa acgtaaatct catccgggaa gagatcggat ttaacgctct
    15301 cgataaattc aaacgaacta ccggcgctct gttgcagctc cagcaggctt tgcatccagc
    15361 gctgggcgcg gatttgcgcg gtggtgctgg tttcgccgtg ctctttataa gcccagtgcg
    15421 cggcaacacc catctccgcc atctgatcca tatcttcggt acggatctgg acctcaaccg
    15481 gcacaccgtg cgggccgatc atcgaggtgt gcaaagactg atagccgttc gcttttggaa
    15541 tggcgatgta gtctttcacg cgacccggac gcggcttgta caggctatgc atttgcccga
    15601 gcacgcgata acaggtgtca gaatcattga cgatcacgcg gaaagcgtag atgtccatga
    15661 ttgagtgaaa acgctgctct ttgagcacca ttttgcagta aatcgaataa agatgctttt
    15721 cgcgaccact gacgcggcac ggtattcccg cttcctgcaa acgcccttcg atttcagaga
    15781 ggattttttg gatcatctct ttacggttgc cgcgcgcggc tttcaccact tctttaatta
    15841 cgcggtaacg attgggatac agcgcctcaa aacccagctc ttcgagttcg gttttaatgt
    15901 ggtggatacc taaacggtgc gccagcgggc tataaatttc gagagtttca cgggcgatgc
    15961 ggcgacgttt gtccgggcga agtgagccca gcgtgcgcat gttgtgggta cggtcggcaa
    16021 gtttgatgag gatgacgcgg atatcctgca ccatcgccat aatcatcttg cgaaagtttt
    16081 cggcctgcgc ctctttctta tcgcggaact tgagtttatc aagtttcgac accccctcta
    16141 ccagctcggc gacgctttta ccaaaaagct gttccatatc ctggtaggtg gcgggagtat
    16201 cttcaatcac gtcatgcagc agcgccgcca tcagcgtttc atagtcgagt ttcatctcgg
    16261 ccagaatgca ggcaaccgct accgggtgcg tgatataggg ttcaccgctt gaacgtgttt
    16321 gcccctcgtg agcatcacgt gcaacgagat acgcctgccg cagacgcttg atttggtctt
    16381 ccggcaggta ggtttgaatc agttgattca ggctttcaaa cagatacaag ggcgacccgc
    16441 tttgtgatta acgacgacct tcagcaatag cggtaacggc ttgtaattca gcggcttcct
    16501 gctcttgctg ttcctggcgt tcgcgaacgt cgaggatctg gttgttgatc agaccttctt
    16561 cgatttcgcg cagcgcgatt acagtggttt tatcgttttc ttccggtacc agcggatcct
    16621 ttccgcctac ctgcatctga cgagcgcgac gcgcggcgac cagtaccagg tcaaaacggt
    16681 taccaatttt ctctacagcg tcctgaacag ttacgcgtgc catacttaaa aagctccaca
    16741 ggtgaagaaa tgactgggca tgatactgaa atcaggttca gtctgccaac aatttgctga
    16801 ttaaagcgtc atgacgctgc ttttggcggc tcatgcgcag acgttcggcg cgaataatgg
    16861 tcttcaaatc ggtcaacgcg gtatcgaagt catcattcac aatcagataa tcatattcgg
    16921 cgtaatggct catttctgca acagcttgcg ccatacgctt tgcaatgacc tcttcgctgt
    16981 cctgaccgcg accgcgtaga cggcggtcca gttcaatttt ggacggcggt aaaataaaga
    17041 tactccgcgc gtgcggcatc ttctggcgaa tttgctgcgc accctgccag tcgatatcga
    17101 gaaaaacatc gacaccggtc gccagtactt gctcaatggc ctcacgcgaa gtgccatagt
    17161 aattaccaaa aacttctgcg tgttcgagga acgcatctct gctaatcatt tctttaaatt
    17221 catcatgatt aacaaagaaa taatgttcac cgtggacttc accaggacgc ggttggcgtg
    17281 tggtgtgtga aacagaaacc tgggtgtcat acaacggttg ggtttttaat aaagcctgaa
    17341 tcaggctgga tttacccgcg ccactggggg cagaaacaat ataaagcgtg ccttgagcca
    17401 tgagtatctt tcgtatgtga ttagcgaaat aaagcctaca tacgagctta ttatacacgg
    17461 cgcggccatg tgacgtagcc cttgctcaca ctttttgcgc aaagttgttg cgttttgcgt
    17521 agctgtttcc gcatttgcct gtgatttact gcaataaaga cgattctcag gaagcgtcct
    17581 cgcagactcc gtttttgcca ctcggctttg ccttcaccac gttgtatatc tgccagcctg
    17641 actacagcgg aggtgaagat gaaagtatgg atggcgatat taataagtat cttgtgctgg
    17701 caatcatctg tgtgggcggt ctgtccggcc tggtcgccag ccagagcaca ggaagaaatt
    17761 tcccgcctgc aacagcaaat aaaacagtgg gacgatgact actggaagga aggaaaaagt
    17821 gaggtggaag acggtgttta cgatcagtta agcgcccgtc ttacgcagtg gcaacgctgc
    17881 tttgggagcg agccccgcga cgtgatgatg ccgcctttaa atggtgcggt tatgcatccg
    17941 gttgcccata cgggtgtacg caaaatggtg gataaaaacg cattaagtct gtggatgcga
    18001 gaacgtagcg atctttgggt gcagccaaaa gttgatggcg ttgcggtaac cctggtttat
    18061 cgggacggga aactgaacaa agcaatcagt cgcggtaatg gcctgaaagg cgaggactgg
    18121 acgcagaaag ttagcttaat ttccgctgtg ccgcaaaccg ttagcgggcc tttagccaac
    18181 agtacgcttc agggggaaat atttctccag cgcgaggggc atatccaaca acaaatgggg
    18241 ggaataaatg cccgcgcaaa agttgctggc ttgatgatgc gccaggacga tagcgacacg
    18301 ctgaattctc tgggcgtttt tgtctgggca tggccggatg gaccgcagtt aatgtctgat
    18361 cgtttaaaag agctggctac cgcaggtttt actctaacgc agacgtatac ccgtgcggtg
    18421 aaaaatgctg atgaagttgc gcgcgtacgc aatgagtggt ggaaagcgga attacccttc
    18481 gtcaccgatg gcgtagttgt gcgaggggcg aaagaacccg aatcccgcca ttggctacca
    18541 ggccaggcag aatggctggt ggcctggaaa tatcaacctg tagctcaggt tgccgaagtg
    18601 aaggcgattc agtttgcggt gggtaagagc ggtaaaatat cggtggttgc gtcactcgca
    18661 cctgtcatgc tggatgataa aaaagtccag cgggtgaata ttggttccgt caggcgctgg
    18721 caggagtggg atattgcgcc tggtgatcag atcctcgtca gccttgccgg tcagggtatt
    18781 cctcgcattg atgatgtggt ctggcgcggt gcagaacgta caaaaccgac accaccagaa
    18841 aaccgcttta actcgttgac ctgctacttt gcttctgatg tttgtcagga acagttcatt
    18901 tcacgcttag tctggctggg gtcaaaacag gttctggggc tggatggcat tggtgaggcc
    18961 ggctggcgcg cgctgcatca gactcatcgc tttgagcata tcttttcctg gcttttatta
    19021 acgccagagc aattacagaa cacgccgggg atcgcgaaaa gtaaaagtgc gcagctatgg
    19081 catcagttta atctggctcg taagcagcct tttactcgct gggtgatggc aatgggaata
    19141 ccgctaaccc gggcggcgct taatgccagt gatgaacggt cctggtcgca acttttattt
    19201 agcacggagc agttctggca gcagctgccg gggactggat cggggcgcgc cagacaggtt
    19261 attgaatgga aggaaaatgc gcaaatcaag aagctgggca gttggctggc tgcccagcag
    19321 atcacaggtt ttgaacctta gtgtccttcg tggctgtagt aaaagaccgg taatcccagc
    19381 ttaaggcgta gcgccagtaa gcgggcaaaa aagccgaata ccagggtgga gataattacc
    19441 acatcatggt tagaaacata gtgttgcagc gcgatgtaca gcacggcgga ggcaaatgag
    19501 acaccggcat acaactcttt ctggaatacc agtgggatac gtttacagaa catatcgcgc
    19561 aaaacgccgc caaacacgcc ggtggttacc gccgcgacaa cggcaataat tgggccgtgc
    19621 cccatatcca gtgcaacctg tgccccgatg atagaaaaga ccaccagtcc gagcgcatcg
    19681 agcaccagaa acactttgcg caggtaaggc attacaggag caacgatagt ggtgagtact
    19741 gcggcggtag cgacgataat cacatattcc gggtgtttga cccagccgag cggatagtgg
    19801 cccagcagaa tatcgcgcac tgaccctccg ccaattgcgg tggcggtcgc aataataatt
    19861 acgccaaatg tatccatgcg gcgtcgcccg gccgccagag cccccgtcat ggcttcggca
    19921 gtaatgccaa ccaaatacaa aatgtgtaac agcatggttc tctccctaaa aatctgtctg
    19981 gcagggtagc gatttgtgcg tattgtcacg attgagattt tttaaggtga gaggaaattg
    20041 ataacttgta gttatgtgaa atcgctattt tctgtagcgt agatgcattc ttctgacttc
    20101 ttgtctgaga aaatgcataa gaaaaaatag gttaaaattg aggggtggaa aagaaaggtg
    20161 caatgtttag ccattgtgtt caccgcacct ggactttggc ttacgcatcg ggtatctcaa
    20221 tcaacaaaaa gagtttttag tttctgctat ttcttctctg taatttttac actgttttcc
    20281 aactgcttga tgctgacctg cggggtcggc aactcctcag gcatggttcc gccaagttcc
    20341 tgaatggttt gcctcacctt gctacccacg tcaaagtgtg tggtattagc ttgctgtttt
    20401 gaattcacgc catcccgctt gagtttttct tctgtttggg tagctcgaaa gagattagcc
    20461 gccagttctg ttgaacccat atgatccagg atcttctgac tctttttcag ccccttcagt
    20521 tggtggatgg ctttctgatc taatccacca tacagcccct ggtaaccatg attctgaaat
    20581 atggcgaaat cagtagctgt cgctacccca gcttgctgtg ctgcctcaac caattgtttg
    20641 ttatgttctt tcaactcatt tctcagaaac agacgctttt cgtcttcacg aagttgctta
    20701 aatgcttcat cgtcagcaag ctcctgccgt cgggtctgga tagcaaaata agtctgccct
    20761 gccgcaatga ccggtttcgc agggtcgccg ttttgtacta ccaggtaaca ggcatagcgg
    20821 gagagatgaa catcttcaag ttctcgttga gcccctgaac ctaatacaac cattttggtg
    20881 gtttcaacga aatggtcaga tgcagcttga ttgctggctt cgcacgcttg agtcgcgcgg
    20941 gccagtacct tttgaaaatt gcgccagtcc cgataatcca gtaacggtgc caattctcta
    21001 gctgaccaaa attcttgccc ttcagtgcca taatgccgta tttcttcaaa aggttgatga
    21061 tgttcgttca ttgcccactc catgtgatat cgattgcaaa gaaagtaaat ctgtaactgt
    21121 atttatatac agttgtgagt atctcacgtt ctcagtgtgc tgggcaaaaa aacatcgcaa
    21181 aatctggagc aggcacgcaa aacagaattg aagttgcggg gaagaaaaat gccgctctgt
    21241 ttgagtgggc ggcatttgtg aaggctgaaa agtaagaatt ggcgttacga gttattcaat
    21301 gttctgaatc tgctcgcgca tctgctcaat caacactttc agctcaatcg cggagtttgt
    21361 cacttcggca ttgatagact tcgacgcaag agtgttcgac tcgcggttga attcctgcat
    21421 cataaagtcc agacgacgac caacggcttc ttttttcttc agaatgttgt aggtctcttt
    21481 gacatgcgct tccaggcggt ccagttcttc ggcaacgtca attcgttgtg ccagcagaac
    21541 cagctcctgc tccagacggt tgttttccaa ctgaacctga gcatcttcca gcttcgcgac
    21601 cagacgctca cgctgccatt gcaggatttc cggcatatgg gcgcggactt tgaccacttc
    21661 ggcggtgacg ccttccagac gctgctcgat caatgctttc agcgcctgac cttcggtttc
    21721 gcgcgcgacg ataaagtcgt ccagcgtacc atccaacgcc gcgagaattt cagcggcaat
    21781 cgcgtcgaga tcctgctcct gggctgccat cacgcccggc cagcgtagaa tatcaaccgg
    21841 gttgatttcc ccttcgtcac tctgcatttt tacccagttc gcggcagtta ccagctgttt
    21901 agccagtttt tcattgagga tcagctcacc ttgtgcgcta acatctggct catagcgcag
    21961 ggtacattcc actttaccgc gcgtcaggcg agaacgaata cgctcgcgaa cgacaggttc
    22021 aaggctacgg aactgctccg gcagacgaaa gtaagtttcc agataacgct ggtttaccga
    22081 gcgcatttcc caggttgcgc tcccccattc acccttgatt tcacgccggg cgtaggcggt
    22141 catactgcgg atcatagacg ttcctgttta taaaaggaga ggtggaagga ttatagccat
    22201 cgatgccttg tcaggatagg aataaccgcc ggaagtccgt ataatgcgca gccacatttg
    22261 tttcaagccg gagatttcaa tatgcgtcca gcaggccgta gcaataatca ggtgcgtccc
    22321 gttaccctga ctcgtaacta tacaaaacat gcagaaggct cggtgctggt cgaatttggc
    22381 gataccaaag tgttgtgtac cgcctctatt gaagaaggcg tgccgcgctt cctgaaaggt
    22441 cagggccagg gctggatcac cgcagagtac ggtatgctgc cgcgttctac ccacacccgt
    22501 aacgctcgtg aagcggcgaa aggtaagcag ggtggacgca caatggaaat ccagcgtctg
    22561 atcgcccgtg ctcttcgcgc ggcagtagat ttgaaagcgc tgggtgagtt caccattacg
    22621 ctggactgcg acgtgcttca ggctgatggt ggcacgcgta ccgcgtcgat tacgggtgcc
    22681 tgcgtggcgc tggcagatgc gctacagaag ctggtggaaa acggcaagct gaaaaccaac
    22741 ccgatgaaag ggatggtagc cgcagtttct gtcggtattg taaacggtga agcaatttgc
    22801 gatctggaat acgttgaaga ctcagccgca gagaccgaca tgaacgtagt gatgaccgaa
    22861 gacgggcgca tcattgaagt gcaggggacg gcagaaggcg agccgttcac ccatgaagag
    22921 ctactcacct tgttggctct ggcccgaggg ggaatcgaat ccattgtagc gacgcagaag
    22981 gcggcgctgg caaactgatt tttaaggcga ctgatgagtc gccttttttt tgtctgtaga
    23041 aaagtaagat gaggagcgaa ggcatgaaac catatcagcg ccagtttatt gaatttgcgc
    23101 ttggcaagca ggtgttaaag tttggcgagt ttacgttgaa gtccgggcgc aaaagcccct
    23161 atttcttcaa cgccgggctg tttaataccg ggcgcgatct ggcactgtta ggccgttttt
    23221 acgctgaagc gttggtggat tccggccttg agttcgatct gctgtttggc cctgcttaca
    23281 aagggatccc gattgccacc actaccgccg tggcgctggc ggagcatcat gaccttgacc
    23341 tgccgtactg ctttaaccgc aaagaggcaa aagaccacgg tgaaggcggc aatctggttg
    23401 gtagcgcgtt acaaggacgc gtaatgctgg tagatgatgt gatcaccgcc ggaacggcga
    23461 ttcgcgaatc gatggagatt attcaggcta atggcgcgac gcttgctggc gtgttgattt
    23521 cgctcgatcg tcaggaacgg gggcgcagcg agatttcggc gattcaggaa gttgagcgtg
    23581 attacaactg caaagtgatc tctatcatca ccctgaaaga cctgattgct tacctggaag
    23641 agaagccgga aatggcggaa catctggcgg cggttaaggc ctatcgcgaa gagtttggcg
    23701 tttaaagaaa ctcgccggat gaaaagtcat ccggcgtcat attactgcaa ctgcgcggca
    23761 attagcggcc agcgggcgtc aaaatcatcc gtcgggcggt atttaaattc gctgcggaca
    23821 aaacgtgaca gcataccttc acagaaggcc aggatctggc ttgccagcag ggtttcatcg
    23881 gtggtgtaac cttcaccctc acgcattctc ttttcacgca atacctggcg cagctgcgct
    23941 tcaatacgct cgaacagctg gttgatgcgc ccttgcaggc gatcctgttc aaacattagc
    24001 gcatgaccag tgaggatgcg ggtcaggcca ggattacgct caccaaaacc gagaagcagc
    24061 aacacaatca gacgcaggcg cgctgtggtg tctttctcat ctttcagaat caggttgatg
    24121 cgagtaatca ggctatcttc gataaactca atcaggctat cgaacatgcg ggtcttactg
    24181 gggaagtggc gatacagtgc cgcttcggaa acgccgacag aggcggccag ttttgccgtc
    24241 gtgatacgtt ggcttccatc gctggattcc agcatcagcg ccagagactg aagtatttcc
    24301 tcgcgacggt tccttttcgc agtttgtttt tctgccatgt tacaaaatac ccctgaaaat
    24361 aagcacttgc caggcggcac ccacgctatg accgcaaacg aaatgtttgc ggctatgtta
    24421 tgacgttatt cggatgcgta tgtgttactg acgaccagag tgaccaaagc cgccttcacc
    24481 gcggtcggtg gcgtcgaaat cttccaccag attaaattca gcctgtacta ccggaacaaa
    24541 aatcatctgg gcgatgcgtt cgccaggttg aatggtgaag ctgtcctgac cacggttcca
    24601 cacggaaatc atcaactggc cctgatagtc ggaatcgatc aatcctacca ggttaccaag
    24661 cacgataccg tgcttatgtc ccaatccgga gcgcggcagc atcattgccg ccagtgaagg
    24721 atcggcaata tgaatcgcca gcccggtcgg aaccagcgta gtgtcacccg gagccagttc
    24781 tacggcgtcg tcgagacagg cacgcaggtc aagtccggca gagccagagg tggcataagt
    24841 cgggagcgga aattccttcc caacgcgcgg gtccagaatc ttaacgtcga tttttttcat
    24901 cataacgggt cacgatctcg tcgagtaata attggccaag gagttctttg cgctcaagcg
    24961 gtaagacttt atctccgtcc tgccagaaaa ggtgtaatgc gttgttgtcg ctgttaaatc
    25021 cttgagttgg ctgggaaaca tcgttcgcgc agatcagatc aaggttttta cggatacgtt
    25081 tttgccgggc gtattcttcc acattatttg tttcggcggc aaatccaacg acgtagggtc
    25141 gatggtcttt tagtgcggca acgcctgcga cgatatcggg gtttttaacc atttttattg
    25201 ttaattcatc accctgcgtg gcctgctttt tgattttctc tggggccacg gtagctgcgc
    25261 gataatccgc cacggcggcg caaccgataa aaatattttg ctgctgtaca gaagcattca
    25321 cggcggcttc catttccagc gcagtcatca catcaacacg tttaacaaac ggtggcgtcg
    25381 gtagtgaaac cggacctgat accagcgtga cgttcgcgcc acgacgggca gcggcggcgg
    25441 cgatagcaaa acccatcttg ccggagctgt gattagagat ataacgcacc ggatcgagcg
    25501 gttcacgcgt cgggccggcg gtaatcataa tgttcagatg tttcaggtcg ttgacgggcg
    25561 aaaaatgcgc caccgccata tccacaatgg ttaacggatc gagcattcgc ccaggaccga
    25621 tatcaccaca agcctgactg ccactgtctg gcccccagat gagcaaacca cgggaagcaa
    25681 gcacctctaa attatgctgc gtggcagcgg cacggtacat ctgctggttc atggcgggga
    25741 gcacggctac aagcgcaggt gtagccagac aaatcgtcga taccaggtca ttcgccattc
    25801 cggcagcaat acgggcaatc aaatctgccg tggcaggggc gagaatcact aaatcagccc
    25861 atttacccag ctcaatatgg cccatagcgg cttcggctgc cgggtccagc agactgtcgg
    25921 aaacgggata accagaaacc gcctgcaagc taagtggggt gataaaggct tttgccgctt
    25981 cggtcatggc tacgcggacg tcggccccgc gatcgcgcaa acgacgcacc agttcagggg
    26041 ttttaaaggc agcaataccg ccgctaacgc cgagaacgat ttttttaccg gccaggctca
    26101 tgatgatttt tcctgttggg tgacaccaga agttggcgat tttatcacaa tacttttgtt
    26161 gtcgtgcctt cacctgagaa tcactttgcg aggcgctttc caggattgaa aactggccgt
    26221 cgatttaacg gaacggctat gacaggatgc gagcaccaca aaggaggtga aggtgaaaaa
    26281 caatgcacag ctgttgatgc cgcgcgaaaa aatgctgaag tttggtatta gcgccttaac
    26341 ggatgtcgag ctgctggcgc tatttctgcg taccggaacg cgcggtaaag atgtattaac
    26401 cctggcaaaa gagatgctgg agaatttcgg ctctctttat ggcttgttaa cctctgaata
    26461 tgaacaattt agtggtgttc atggaattgg cgtagcgaaa tttgcccagt taaaggggat
    26521 tgctgaactg gcgcgacgtt actacaacgt gcggatgcgt gaagaaagcc ctttactcag
    26581 cccggagatg acgcgggaat ttttacaaag ccagctcacg ggtgaggagc gggagatctt
    26641 tatggtgatc tttctcgact cacaacaccg ggttataacg catagccgtc ttttttccgg
    26701 cacgctaaac catgttgaag tccatcctcg ggaaattatc cgcgaagcga taaaaataaa
    26761 cgcctcggcg ctgatccttg cacataatca cccttcgggt tgtgctgaac ccagtaaagc
    26821 ggataaactc attactgaac ggataataaa gagttgtcag ttcatggatt tacgcgtgct
    26881 cgaccatatc gtgattgggc gtggagagta tgtttctttt gccgaacgcg gctggattta
    26941 acccgctatg cgcgatcctt cgggatcttt gtctgttcgg gacttgagca catcgctgag
    27001 tcagcgtata ctacgccacc tttgagaatc tcgggtttgg catttgggcc tggcaatcga
    27061 gagttcacat agaactgcga tgaccgggct gtaaagcctg acgaggcgcc aataccccat
    27121 acgaagctcg agctaatttg atttttggag aatagacatg tcccgagtct gccaagttac
    27181 tggcaagcgt ccggtgaccg gtaacaaccg ttcccacgca ctgaacgcga ctaaacgccg
    27241 tttcctgccg aacctgcact ctcaccgttt ctgggttgag agcgagaagc gttttgtcac
    27301 cctgcgcgta tctgctaaag gtatgcgtgt aatcgataaa aaaggcatcg atacagttct
    27361 ggctgaactg cgtgcccgtg gcgaaaagta ctaagtactt agaggaaata aatcatggct
    27421 aaaggtattc gtgagaaaat caagctggtt tcttctgctg gtactggtca cttctatacc
    27481 actacgaaga acaaacgtac taagccggaa aaactggaac tgaaaaaatt cgatccagtt
    27541 gttcgccagc acgtgatcta caaagaagcg aaaatcaaat aattctcgct ttgatgtaac
    27601 aaaaaacctc gctccggcgg ggttttttgt tatctgcttg cccccatatt gactgcatct
    27661 gttcattcct ggagatgcta tgcctgaatt acccgaagtt gaaaccagcc gccgcggcat
    27721 agaaccgcat ctcgttggtg caaccattct tcatgcagtg gtgcgcaacg gacgcttgcg
    27781 ctggccggtt tcagaagaga tctaccgttt aagcgaccaa ccagtgctta gcgtgcagcg
    27841 gcgggctaaa tatctgctgc tggagctgcc tgagggctgg attatcattc atttagggat
    27901 gtctggcagc ctgcgcatcc ttccagaaga acttccccct gaaaagcatg accatgtgga
    27961 tttggtgatg agcaacggca aagtgctgcg ctacaccgat ccgcgccgct ttggtgcctg
    28021 gctgtggacc aaagagctgg aagggcataa tgtgctgacc catcttggac cggagccgct
    28081 tagcgacgat ttcaatggtg agtatctgca tcagaagtgc gcgaagaaaa aaacggcgat
    28141 taaaccgtgg ctgatggata acaagctggt ggtaggggta gggaatatct atgccagcga
    28201 atcactgttt gcggcgggga tccatccgga tcggctggcg tcatcactgt cgctggcaga
    28261 gtgtgaattg ttagctcggg tgattaaagc ggtgttgctg cgttcgattg agcagggtgg
    28321 tacaacgctg aaagattttc tgcaaagtga tggtaaaccg ggctatttcg ctcaggaatt
    28381 gcaggtttac gggcgaaaag gtgagccgtg tcgggtgtgc ggtacgccga ttgtggcgac
    28441 taaacatgcg cagcgggcaa cgttttattg tcggcagtgc cagaagtaat tcatgcgcgc
    28501 cggatggcat accatccggc ataaacgcta cgctaacttc gccatcagcg cctgatggac
    28561 attctccggc aggaaatggg tgacatcgcc ctgatggcgt gccacctctt tcaccaacga
    28621 tgaagagata aacgaccact ctttcgacgg catcagaaac acactttcca gttccggcat
    28681 taagtggcga ttcatatgcg ccagctgcat ttcatattca aaatccgcca ccgcacgcag
    28741 gccacgaatc agcaccgtag cgtgttgatt acgggcgaag ttcgccatca aatcactaaa
    28801 cccgaccact tccacgttcc ccagatgcgc ggttgcctgc tgtgccagtg ccacacgctc
    28861 ttccagggta aacatcggtt ttttactggg gctggcggca atcgccagaa taacgtgatc
    28921 gaacatctgc gtggcgcgcg tcacgatatc gatatgacca ttggtaatgg gatcgaaagt
    28981 acccggataa atcgcccgtt tttgcataac aacctcaatg cgttttcggt ggcaggtaag
    29041 gttccagcag ttgaagcaga cgctgtagcg cgccctggtt ttgatacagt acttcaacgg
    29101 catgacggcc atagaaacta cggtaatcgg cgtcggtgag taaagaggac acctcttttg
    29161 caagcgtagt ggcatcggta acggtaatca gcccgcttgc ctgctccagc cgcgcgcaaa
    29221 tgtctttaaa gttaaaagta tgcggcccca tcaataccgg aatagcgtgt gcggcagctt
    29281 ccagcggatt atgcccacca cgttcaacca gtgaaccgcc aacaaaggcg agatcggcaa
    29341 tgccgtacag taacatcaac tcgcccatcg tatcgccaac tacaacctgc gtgctggtgg
    29401 aggggacttc ccctgaagag cgtgtgatat agcttagtcc agcctggcgg acaaggttaa
    29461 tcgcatccgg gaagcgttct ggatgacggg gtaccaggat gagcaataaa ttcgggaatt
    29521 gctgtaacaa tgcctgatgt gcggcaatga ccacgctctc ttcaccttcg tgagtgctgg
    29581 tggcaatcca taccgggcgg tgtggtgccc attggcggcg tagcgtcacg gctttagcag
    29641 ccaactgcgg cgttacagaa atatcgaatt tcaggctacc ggtaacggtt acctggttat
    29701 tttttgcgcc cagcgccaca aaacgtgcac catcttcttc attttgcgca gcaatcagcg
    29761 taatacgacg cagcaagcgg cggacgaatt tacccagttt ggcataacct gcggccgagc
    29821 gggcagagag tcgcgcgtta gcgatcacca gcggaatttt acgtttatgt agcgccgcaa
    29881 tcaggttagg ccatagttcg gtttccataa tcaacaccag tttagggtcg actttattca
    29941 ggaaacggtt gagtgcatcg ggcaggtcat acggcagata aacgtgctga acatccttcc
    30001 cgaaagccga ttgtacgcgc tccgaaccgg ttggcgtcat ggttgtaacg gtaatcggta
    30061 aatcaggata acgatggcgc agcgcgcgca ccaacgggat cgccgccaga gtttcaccga
    30121 cggagacgga gtgcagcata atgccgcctg gttttagcgg atggcggtaa aaaccgtaac
    30181 gttcacccca gcgttttcga taggccggag ccttacgtcc gcgcacccag agccgtatcc
    30241 agatcagcgg ctgaataagg tagagaaggg cggtgtaaag caattcgagc atagtaaata
    30301 gctgacttat ggatgtgctg gggattctat gtatttagct gtggctttac cattactttt
    30361 cccgtttttg acttaaatag caatttaccc gccaattctt gctgaacatc tatacttttt
    30421 ttgtattttt atgcgattca ttgatttctg ctgtacgtgc aggaaggaat cgcagagcaa
    30481 cagtgaaaga atatggttgc taatgatttt atttgattga tttctttatt gaaaatcaac
    30541 aatgtgctta aaaatcattc gggtcggatt gccgcgaaag aagtgataca ctagcgcgtc
    30601 aatatcagtg cggtatcagt attcaggtag ctgttgagcc tggggcggta gcgtgctttt
    30661 tctgcttcac cttaatcgga taatctcaac aaaaagagtt acttgtggat aagccatttc
    30721 gaagaatttt gctcattaag atgcgttttc atggggatat gttattaact actcccgtca
    30781 ttagttcgct gaaaaaaaat taccctgacg caaaaatcga tgtgctgctt tatcaggaca
    30841 ccatcccgat cctgtctgaa aatccagaga ttaacgcgct ctacggcata aaaaataaaa
    30901 aagcaaaagc ctcagaaaaa attgccaact tttttcatct catcaaggta ttacgtgcca
    30961 ataagtatga ccttatcgtc aatcttactg atcaatggat ggttgctata ctggttcgct
    31021 tattaaatgc ccgtgtgaaa atttcccagg attatcatca tcggcagtct gctttttggc
    31081 gtaaaagttt cacccatttg gtgccgttgc agggtggaaa tgtggtggaa agtaacttat
    31141 ccgtgctgac accattggga cttgattcgt tggtgaagca gacaaccatg agttacccgc
    31201 ctgcaagctg gaaacgtatg cgtcgcgaac ttgatcacgc tggtgttgga caaaattatg
    31261 tggttatcca acctacggcg cggcaaatct tcaaatgctg ggacaacgcc aagttttccg
    31321 ctgtgattga tgccttacat gctcgtggtt atgaagtcgt tctgacgtcc ggcccagata
    31381 aagacgatct ggcctgcgtc aatgaaattg cgcagggatg ccagacgcca ccagtaacgg
    31441 cgctggctgg aaaggtgacc ttcccggaac ttggtgcgtt aatcgatcat gcgcagctgt
    31501 ttattggcgt tgattccgca ccggcgcata ttgccgctgc agttaatacg ccgctgatat
    31561 cgctgtttgg cgcaacagat catattttct ggcgtccctg gtcaaataac atgattcaat
    31621 tctgggcggg agattaccgg gaaatgccaa cgcgcgatca gcgtgaccga aatgagatgt
    31681 atctttcggt tattccggcg gcagatgtca ttgctgctgt cgataaatta ctgccctcct
    31741 ccacgacagg tacgtcgtta tgatcgttgc tttttgttta tataaatatt ttccctttgg
    31801 cggtttgcag cgcgatttta tgcgtattgc tcagacagtc gccgcccgag gtcatcatgt
    31861 tcgggtttat acccagtcgt gggaaggcga atgccctgat gtatttgaac tgatcaaagt
    31921 gccggttaaa tcgcatacca atcacgggcg caatgcggag tattttgcct gggtgcaaaa
    31981 acatttacgc gaacatcccg tcgataaagt cgttggattc aacaaaatgc cggggctgga
    32041 cgtttattat gccgctgatg tttgttatgc cgagaaagta gcgcaggaaa aaggcttttt
    32101 ctatcgcctg acgtcacgtt atcgccatta tgccgccttt gagcgggcaa ccttcgaaca
    32161 gggcaagccg acacagctgc tgatgctgac agataagcaa atcgccgatt tccagaaaca
    32221 ttatcagact gaagcggagc gttttcatat tctgccaccg gggatttatc ctgatcgtaa
    32281 atatagccag cagccagcca atagccgtga aatcttccgt aagaagaatg gaataaccga
    32341 acaacaatat ttattgttgc aggtcggttc agacttcacg cgtaaaggtg tcgatcgttc
    32401 cattgaagca cttgcttcgt taccggattc gctgcgccac aacacattgc tatatgttgt
    32461 tgggcaggat aaaccgcgaa aatttgaggc gctggcagaa aaacgcggcg tgcgcagtaa
    32521 tgttcacttt ttctcggggc gcaatgatgt ctcggaatta atggcggcgg cggatttatt
    32581 actgcatcct gcctaccagg aagcggcggg aattgtgctg ctggaagcga ttactgcagg
    32641 attaccggta ctaacaacag ccgtttgtgg ctatgcgcat tatattgtcg acgccaattg
    32701 cggcgaggct attgctgagc cattccgcca ggaaacattg aatgagattt tacgcaaagc
    32761 gttaacgcaa tcttcattgc gccaggcctg ggcggaaaat gcgcgacatt atgctgatac
    32821 acaagattta tacagtctgc cagagaaagc ggcggatatc ataacgggtg gtctggatgg
    32881 ttgaacttaa agagccgttt gccacgttat ggcgcggtaa agatcctttt gaggaagtta
    32941 aaaccttgca gggtgaggta tttcgtgaac tggaaactcg ccgtactctg cgctttgaaa
    33001 tggcgggcaa aagctatttt ctcaaatggc atcgcggcac gaccctgaaa gagataatca
    33061 aaaatttact ctcattgcgg atgccagtat taggcgctga ccgcgaatgg aatgcgattc
    33121 atcgactgcg ggatgtcggc gttgatacta tgtatggggt ggcatttggc gaaaaaggca
    33181 tgaatccgct gaccagaact tcatttatta ttaccgaaga tctgacacca accataagtc
    33241 tggaagatta ctgtgctgac tgggcgacta accctccaga tgttcgcgta aagcgtatgc
    33301 ttattaagcg tgtcgcgacg atggtgcgcg atatgcatgc tgcgggcatt aaccaccgtg
    33361 actgttatat ctgtcatttc ctgctgcact tgcctttttc cggtaaggaa gaggagttaa
    33421 aactttcagt aattgacctg caccgggcgc agcttcgcac gcgcgttcca cgtcgttggc
    33481 gggataaaga tcttattggg ctttattttt cttcgatgaa tatcggcctg actcagcggg
    33541 atatctggcg gtttatgaaa gtgtattttg ccgccccgct taaagacatt ctcaagcagg
    33601 aacaaggact gctgtcgcaa gcagaagcaa aagccacaaa aatcagggaa agaacgattc
    33661 gaaaatcgtt gtaatttccg gagagaaata tgagtgccca ctattttaat ccacaagaga
    33721 tgatcaataa gacaatcatc ttcgatgaaa ggccagcggc gtcagtggca tcatcattcc
    33781 atgttgctta tggcattgat aaaaactttc tttttggttg tggtgtttca atcacgtcag
    33841 ttttgttaca taacaacgac gtgagttttg ttttccacgt ttttattgat gatatccctg
    33901 aagccgatat ccagcgttta gcccaattgg cgaaaagcta tcgtacctgt atccagatcc
    33961 atctagtaaa ttgtgaacgg cttaaggcat taccgacgac caaaaattgg tctattgcca
    34021 tgtatttccg ttttgtaatt gcagattact ttattgatca acaagataag atcctttacc
    34081 tggatgctga tatcgcctgt cagggaaact taaagccgct gataacaatg gatcttgcca
    34141 ataacgttgc tgctgttgtt actgaacgcg atgctaactg gtggtcgtta cggggtcaaa
    34201 gtctgcagtg taatgaactt gaaaagggtt actttaattc aggtgtcctg ttaattaata
    34261 cactagcgtg ggcgcaggag tccgtttctg ctaaagcgat gtcgatgctt gctgataaag
    34321 ccatcgtttc ccgtttaacc tatatggatc aagatatcct taatcttatc ctgttaggga
    34381 aagttaaatt cattgatgct aaatacaata cgcaatttag tttaaattat gaattaaaaa
    34441 aatcatttgt ttgtc
//
LOCUS       DAPGWD010000033        34415 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPGWD010000033 DAPGWD010000000
VERSION     DAPGWD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.033"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..320)
                     /locus_tag="RHX88_001569"
     CDS             complement(<1..320)
                     /locus_tag="RHX88_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230268.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLR"
     gene            complement(480..692)
                     /gene="rpmE"
                     /locus_tag="RHX88_001570"
     CDS             complement(480..692)
                     /gene="rpmE"
                     /locus_tag="RHX88_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDV2230269.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            895..3093
                     /gene="priA"
                     /locus_tag="RHX88_001571"
     CDS             895..3093
                     /gene="priA"
                     /locus_tag="RHX88_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDV2230270.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3249..4274
                     /gene="cytR"
                     /locus_tag="RHX88_001572"
     CDS             3249..4274
                     /gene="cytR"
                     /locus_tag="RHX88_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDV2230271.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4366..5325
                     /gene="ftsN"
                     /locus_tag="RHX88_001573"
     CDS             4366..5325
                     /gene="ftsN"
                     /locus_tag="RHX88_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDV2230272.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5418..5948
                     /gene="hslV"
                     /locus_tag="RHX88_001574"
     CDS             5418..5948
                     /gene="hslV"
                     /locus_tag="RHX88_001574"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDV2230273.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5958..7289
                     /gene="hslU"
                     /locus_tag="RHX88_001575"
     CDS             5958..7289
                     /gene="hslU"
                     /locus_tag="RHX88_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDV2230274.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7356..8282
                     /gene="menA"
                     /locus_tag="RHX88_001576"
     CDS             7356..8282
                     /gene="menA"
                     /locus_tag="RHX88_001576"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDV2230275.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8375..8860
                     /gene="rraA"
                     /locus_tag="RHX88_001577"
     CDS             8375..8860
                     /gene="rraA"
                     /locus_tag="RHX88_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDV2230276.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8945..9190)
                     /gene="zapB"
                     /locus_tag="RHX88_001578"
     CDS             complement(8945..9190)
                     /gene="zapB"
                     /locus_tag="RHX88_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDV2230277.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9616..10461
                     /gene="glpF"
                     /locus_tag="RHX88_001579"
     CDS             9616..10461
                     /gene="glpF"
                     /locus_tag="RHX88_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDV2230278.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10484..11992
                     /gene="glpK"
                     /locus_tag="RHX88_001580"
     CDS             10484..11992
                     /gene="glpK"
                     /locus_tag="RHX88_001580"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDV2230279.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12128..13138
                     /gene="glpX"
                     /locus_tag="RHX88_001581"
     CDS             12128..13138
                     /gene="glpX"
                     /locus_tag="RHX88_001581"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV2230280.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13235..13981
                     /gene="fpr"
                     /locus_tag="RHX88_001582"
     CDS             13235..13981
                     /gene="fpr"
                     /locus_tag="RHX88_001582"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDV2230281.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13986..14414)
                     /gene="uspD"
                     /locus_tag="RHX88_001583"
     CDS             complement(13986..14414)
                     /gene="uspD"
                     /locus_tag="RHX88_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDV2230282.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14441..14740)
                     /locus_tag="RHX88_001584"
     CDS             complement(14441..14740)
                     /locus_tag="RHX88_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDV2230283.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14952..15392)
                     /locus_tag="RHX88_001585"
     CDS             complement(14952..15392)
                     /locus_tag="RHX88_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV2230284.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWLAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15493..16092
                     /locus_tag="RHX88_001586"
     CDS             15493..16092
                     /locus_tag="RHX88_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDV2230285.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16200..16967
                     /gene="tpiA"
                     /locus_tag="RHX88_001587"
     CDS             16200..16967
                     /gene="tpiA"
                     /locus_tag="RHX88_001587"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDV2230286.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17022..17777)
                     /gene="cdh"
                     /locus_tag="RHX88_001588"
     CDS             complement(17022..17777)
                     /gene="cdh"
                     /locus_tag="RHX88_001588"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDV2230287.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17884..18873)
                     /gene="sbp"
                     /locus_tag="RHX88_001589"
     CDS             complement(17884..18873)
                     /gene="sbp"
                     /locus_tag="RHX88_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDV2230288.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19193..20155)
                     /gene="pfkA"
                     /locus_tag="RHX88_001590"
     CDS             complement(19193..20155)
                     /gene="pfkA"
                     /locus_tag="RHX88_001590"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDV2230289.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20336..21238)
                     /gene="fieF"
                     /locus_tag="RHX88_001591"
     CDS             complement(20336..21238)
                     /gene="fieF"
                     /locus_tag="RHX88_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDV2230290.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21387..21887)
                     /gene="cpxP"
                     /locus_tag="RHX88_001592"
     CDS             complement(21387..21887)
                     /gene="cpxP"
                     /locus_tag="RHX88_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDV2230291.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22037..22735
                     /gene="cpxR"
                     /locus_tag="RHX88_001593"
     CDS             22037..22735
                     /gene="cpxR"
                     /locus_tag="RHX88_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDV2230292.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22732..24105
                     /gene="cpxA"
                     /locus_tag="RHX88_001594"
     CDS             22732..24105
                     /gene="cpxA"
                     /locus_tag="RHX88_001594"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDV2230293.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24211..24885)
                     /gene="yiiM"
                     /locus_tag="RHX88_001595"
     CDS             complement(24211..24885)
                     /gene="yiiM"
                     /locus_tag="RHX88_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDV2230294.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25034..26017)
                     /gene="kdgT"
                     /locus_tag="RHX88_001596"
     CDS             complement(25034..26017)
                     /gene="kdgT"
                     /locus_tag="RHX88_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDV2230295.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26277..26897)
                     /gene="sodA"
                     /locus_tag="RHX88_001597"
     CDS             complement(26277..26897)
                     /gene="sodA"
                     /locus_tag="RHX88_001597"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDV2230296.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27182..28216
                     /gene="rhaT"
                     /locus_tag="RHX88_001598"
     CDS             27182..28216
                     /gene="rhaT"
                     /locus_tag="RHX88_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDV2230297.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28213..29079)
                     /gene="rhaR"
                     /locus_tag="RHX88_001599"
     CDS             complement(28213..29079)
                     /gene="rhaR"
                     /locus_tag="RHX88_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDV2230298.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(29135..29971)
                     /gene="rhaS"
                     /locus_tag="RHX88_001600"
     CDS             complement(29135..29971)
                     /gene="rhaS"
                     /locus_tag="RHX88_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDV2230299.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30259..31728
                     /gene="rhaB"
                     /locus_tag="RHX88_001601"
     CDS             30259..31728
                     /gene="rhaB"
                     /locus_tag="RHX88_001601"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDV2230300.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31725..32984
                     /gene="rhaA"
                     /locus_tag="RHX88_001602"
     CDS             31725..32984
                     /gene="rhaA"
                     /locus_tag="RHX88_001602"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDV2230301.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            33226..34050
                     /gene="rhaD"
                     /locus_tag="RHX88_001603"
     CDS             33226..34050
                     /gene="rhaD"
                     /locus_tag="RHX88_001603"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDV2230302.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34060..34374
                     /gene="rhaM"
                     /locus_tag="RHX88_001604"
     CDS             34060..34374
                     /gene="rhaM"
                     /locus_tag="RHX88_001604"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDV2230303.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         8398 a         8971 c         8968 g         8078 t
ORIGIN      
        1 cgcagctgta agcggatatc cgcaggcatt ttccagccgg ggccgaggct ccccaccggc
       61 gcgggcgttt ttgtccggta actgctgtag gtgcgggaga ggatgaacgg cagcgggccg
      121 ggcagggcga tgtcggtttc accgggaagg acctttgcac cgagcagggg attgaccgga
      181 tggccggacg tcactccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccgatg
      241 cgcaccccgg ctgaaccctg gacaatgcta ccgccatact gcgtcatgtc gccctgacgc
      301 gctgccggtt ttccgctcat cgtaaaatcc ttttgaaata aataagaacc agaagcctag
      361 cgccagtcaa caacaggaaa tgataatgag ttaacacccc ggaatttttc agaatttata
      421 aaaggcaaat tacaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct
      481 tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc accggtagca
      541 acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc gaggttcagg
      601 tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct agcagtaatt
      661 tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg
      721 ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc aatacgatca
      781 tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac tgatccgcac
      841 tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga tgctatgccc
      901 gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct gctgccagaa
      961 ggcatgacgg ttaaagccgg gtgtcgcgtg cgcgtgccgt ttggcaaaca gcaggagcgc
     1021 atcggggttg tggtgtcagt tagcgatgtc agtgagctgc cgcttaacga gctgaaagcg
     1081 gtagttgaag tgctggacgt tgagccggta tttactcact ccgtctggcg gttactgctc
     1141 tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt
     1201 ttactgcgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa
     1261 caaggtcagg cggtggatct taacagcctg aaacgttcgc caaagcaaca acaggcgctg
     1321 gcggcgttac ggcaaggcaa aatctggcgc gaccaggtcg cggaacttga atttaatgat
     1381 gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag
     1441 tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt gaataccgaa
     1501 caagccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc ctggctgctg
     1561 gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga aaacgtgctc
     1621 gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc gcaaactatc
     1681 gcccgttttc gtgaacgttt taatgccccc gtggaagttc tgcattccgg cctgaacgac
     1741 agcgagcggc tttcggcgtg gttgaaagcg aaaaatggcg aggcggcgat tgtgatcggc
     1801 acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat tgatgaagag
     1861 cacgacagct cctacaagca gcaggaaggc tggcgctatc atgcccgcga cctggcggtg
     1921 tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc cgcgctggaa
     1981 acgttatgca acgtccagca gaaaaaatac cgcctgctgc gcctgacccg tcgggcaggg
     2041 aatgcgcgtc cggcaattca acatgtgctg gatttaaaag gtcagaaggt gcaggcaggt
     2101 ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa tcaggttatc
     2161 ctctttctta atcgtcgggg ttttgcgcct gcgctgctgt gccacgactg cggctggata
     2221 gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca gcatttgcgc
     2281 tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg cggttccacg
     2341 cacctggtcc ccgtggggct gggcaccgaa cagcttgaac agacgcttgc gccgatgttc
     2401 cccggcgtgc ccatttcacg tatcgaccgc gataccacca gccgcaaagg ggcgctggaa
     2461 cagcaactgg cggaagtgca tcgcggcggc gcgaggatct tgattggcac gcaaatgctg
     2521 gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt ggacggcgcg
     2581 ctgttttctg ccgattttcg ctcggcagaa cgtttcgccc agctttacac ccaggtcgcc
     2641 ggtcgtgccg ggcgtgcggg taaacagggc gaagtggtgc tgcaaacgca ccatccggaa
     2701 catcctctgt tgcaaacgtt gctctataaa ggctacgacg cctttgccga acaggcgcta
     2761 gctgagcgac gcatgatgca gctgccgccg tggaccagcc atgtgattgt gcgtgcggaa
     2821 gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct gatcctttcc
     2881 agcccgctgg cagacgacaa actgtgggtt ctcggcccgg ttccggctct ggcacctaag
     2941 cgtggtggcc gctggcgctg gcagatcttg ttgcagcacc cttcccgcgt gcgcttgcaa
     3001 cacatcatta gtggtacgct ggcgcttatt aatacaatac cggattcccg caaggtgaaa
     3061 tgggtgttgg atgtcgatcc catcgaaggc taatgcgcaa acgttgcgag gcggatcgaa
     3121 aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac gcgctatctg
     3181 ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt gccaggcgag
     3241 gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac gttgccctca
     3301 aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat aaagtctccc
     3361 aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta ccgcaaccta
     3421 tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc ccggatatct
     3481 gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca aatcacggat
     3541 atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt
     3601 tgatcatcac caagcaaatt gatggcatgt tgctgctggg ctcaaggctg ccgtttgatg
     3661 ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa tttgcaccgg
     3721 aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat gcagtaaatt
     3781 atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa gagatgccac
     3841 tgtgtcacta tcgcctgcaa ggctatgttc aggcgctgcg tcgctgcggc attatggttg
     3901 atccgcaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa gcgatgcagc
     3961 agctgcttga tcttccacaa ccgcctactg ctgtcttctg ccatagcgat gtgatggcgc
     4021 tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac ctttccataa
     4081 tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc
     4141 agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa atgcaggggc
     4201 aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg ggatcaacac
     4261 gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc ccgccgctta
     4321 agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc acaacgagat
     4381 tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag
     4441 caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg
     4501 acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc cgagacgctg
     4561 caaagccaga aagtgaccgg taacggacta ccaccaaaac cagaagaacg ctggcgctac
     4621 attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc ttctgccggt
     4681 ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct tgaacaaatg
     4741 caggctgata tgcgccagca gccaacgcag ctggttgaag ttccgtggaa tgaacagacg
     4801 ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt agcggagcag
     4861 caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca gacgcgtacg
     4921 tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccggcttc tactcagcag
     4981 ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa gccgcagcaa
     5041 gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa
     5101 cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga gacggtacgt
     5161 gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg ctggaatcgt
     5221 gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa tcggttgaag
     5281 atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc tcaaaatccc
     5341 ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt
     5401 aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc catgtggtca
     5461 tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg
     5521 tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact gcggatgctt
     5581 ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat ctggtcaaag
     5641 ccgccgttga gctggcgaaa gactggcgta ccgatcgcat gctgcgcaaa cttgaagcac
     5701 tgctggcagt cgcggatgaa actgcatcgc ttatcatcac cggtaacggt gacgtggtgc
     5761 agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag gctgcggcgc
     5821 gtgcactgtt agaaaatact gaactgagtg ctcgtgaaat tgctgaaaag gcgttggata
     5881 ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa ttaagctaca
     5941 aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc gaactggata
     6001 agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg cgtaaccgct
     6061 ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa aatatcctga
     6121 tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct aagctggcga
     6181 atgcgccgtt catcaaagtt gaagcgacca aattcaccga agtaggctac gtcggtaagg
     6241 aagtggattc tattattcgc gatctgaccg atgccgccgt gaaaatggta cgcgtccagg
     6301 ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt ctcgacgtgc
     6361 tgatcccacc tgctaaaaac aactggggac agaccgaaca gcagcaggaa ccgtccgctg
     6421 ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga
     6481 tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccaggc atggaagaga
     6541 tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa aaagcgcgta
     6601 agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg aaactggtga
     6661 acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg atcgtgttta
     6721 tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg
     6781 aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc accaaacacg
     6841 ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag attgcgaaac
     6901 cgtctgacct gatcccggaa ctgcaaggtc gtctgccaat ccgcgttgaa ctgcaggcgc
     6961 tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc accgtgcagt
     7021 acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc ggtattaagc
     7081 gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt gctcgtcgtt
     7141 tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc gatttaagcg
     7201 gtcaaactat catcattgac gcagattatg tgagcaaaca tctggatgcg ttggtggcag
     7261 atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc attgtttgat
     7321 ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa attagccgaa
     7381 ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt gctgcaatta
     7441 tcgtcgggac agcgctggca tggtggcaag gtcacttcga tccgctggtc gccctgctgg
     7501 cactgattac cgccgggcta ttacagatcc tttctaacct cgccaatgat tacggcgatg
     7561 cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg caaaaagggg
     7621 tcattaccca gcaagagatg aaacgggcgc tcattattac cgtcgtgctc atctgtctct
     7681 ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc ggtttcctga
     7741 ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat cgtccttatg
     7801 gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg agtgtcatgg
     7861 ggagctggta tttacaggct catacattga ttccggcact gatccttccg gcgaccgcat
     7921 gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc aatagcgacc
     7981 gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg cgtcgttatc
     8041 atgcctgcct gctgatgggc tcactggtgt gtctggcgct gtttaatctc ttttcgctgc
     8101 atagcctgtg gggctggctg ttcctgctgg cggcaccatt actggtgaag caagcccgtt
     8161 atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt actgtcaagg
     8221 gagcgttact gactaacctg ctgtttattt tagggatatt cctaagccag tgggcagcat
     8281 aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata gcgcgatata
     8341 ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt ccgagctttg
     8401 tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg
     8461 ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg ggttgctgta
     8521 cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg gtggttctgt
     8581 tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg aatgggaagg
     8641 tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg atatcggcat
     8701 ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt
     8761 ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg ccgacaatac
     8821 cgggattatt ctttcagaag atccgctgga tattgaatga tagaaaaggc accgcaaggt
     8881 gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga aagatgaagc
     8941 gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct
     9001 gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt
     9061 gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca
     9121 gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctaatg
     9181 acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc tcgtcccgat
     9241 taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg tatgaagccg
     9301 cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt ttcatccttc
     9361 tcctggtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg cgaatgatct
     9421 aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc tcattttatg
     9481 acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac gataagttta
     9541 cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca aacattaact
     9601 cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat tgctgaattc
     9661 ctcggtaccg ggttgttgat tttcttcggt gtgggttgcg ttgcagcact aaaagtcgct
     9721 ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt ggcaatggcc
     9781 atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac cattgcattg
     9841 tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc acaagttgcc
     9901 ggcgctttct gtgctgcggc tttagtttac gggctttact acaatttatt tttcgacttc
     9961 gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc tggcactttc
    10021 tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga gatggtgatt
    10081 accgctattc tgatggggct gatcctggcg ttaacggacg atggcaacgg tgtaccacgc
    10141 ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc atctatgggc
    10201 ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt ctttgcctgg
    10261 ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta cttcctggtg
    10321 ccgcttttcg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt
    10381 ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac aactccttca
    10441 gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg aaaaaaaata
    10501 tatcgttgcg ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg atcacgatgc
    10561 caatatcatt agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac caggttgggt
    10621 agaacacgac ccaatggaaa tctgggccac ccaaagctcc acgctggtag aagtgctggc
    10681 gaaagccgat atcagttccg atcaaattgc agctatcggt attacgaacc agcgtgaaac
    10741 cactattgtc tgggaaaaag aaaccggcaa gcctatctat aacgccattg tctggcagtg
    10801 ccgtcgtacc gcagaaatct gcgagcattt aaaacgtgac ggtttagaag attatatccg
    10861 cagcaatacc ggtctggtga ttgacccgta cttttctggc accaaagtga agtggatcct
    10921 cgaccatgtg gaaggctctc gcgagcgtgc acgtcgtggt gaattgctgt ttggtacggt
    10981 tgatacgtgg cttatctgga aaatgactca gggccgtgtc catgtgaccg attacaccaa
    11041 cgcctctcgt accatgttgt tcaacatcca taccctggac tgggacgaca aaatgctgga
    11101 agtgctggat attccgcgcg agatgctgcc agaagtgcgt cgttcttccg aagtatacgg
    11161 tcagactaac attggcggca aaggcggcac gcgtattcca atctccggga tcgccggtga
    11221 ccagcaggcc gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga agaacaccta
    11281 tggcactggc tgctttatgc tgatgaacac tggcgagaaa gcggtgaaat cagaaaacgg
    11341 cctgctgacc accatcgcct gcggcccgac tggcgaagtg aactatgcgt tggaaggtgc
    11401 ggtgtttatg gcaggcgcat ccattcagtg gctgcgcgat gagatgaagt tgattaacga
    11461 cgcctacgat tccgaatatt tcgccaccaa agtgcaaaac accaatggtg tgtatgtggt
    11521 tccggcattt accgggctgg gtgcgccgta ctgggacccg tatgcgcgcg gggcgatttt
    11581 cggtctgact cgtggggtga acgctaacca cattatacgc gcgacgctgg agtctattgc
    11641 ttatcagacg cgtgacgtgc tggaagcgat gcaggccgac tctggtatcc gtctgcacgc
    11701 cctgcgcgtg gatggtggcg cagtagcaaa caatttcctg atgcagttcc agtccgatat
    11761 tctcggcacc cgcgttgagc gcccggaagt tcgcgaagtg actgcattgg gtgcggccta
    11821 tcttgctggt ctggcggtcg gtttctggca gaacctcgac gagctgcaag agaaagcggt
    11881 gattgaacgc gagttccgtc caggcatcga aaccactgag cgtaattacc gttacgcagg
    11941 ctggaaaaaa gcggtcaaac gcgcgatggc gtgggaagaa cacgacgagt aatgtaaatg
    12001 ccggatgaag cgtttatgcc gcatccggta gtcctgaaac cgtgcggggg caaccccgca
    12061 cacatcaata atcccaccct tcccctgtgc tacacttcgc gccattcctt actgcttaga
    12121 gtttgctatg agacgagaac ttgccatcga attttcccgc gtcaccgaat ctgcggcgct
    12181 ggctggctac aaatggttag gacgcggcga taaaaacacc gcggacggcg cggcggtaaa
    12241 cgccatgcgt attatgctca accaggtcaa cattgacggc accatcgtca ttggtgaagg
    12301 tgaaatcgac gaagcaccga tgctctacat tggtgaaaaa gtcggtactg gtcgcggcga
    12361 cgcggtagat attgctgttg atccgattga aggcacgcgc atgacggcga tgggccaggc
    12421 taacgcgctg gcggtgctgg cagtaggcga taaaggctgc ttcctcaatg cgccggatat
    12481 gtatatggag aagctgattg tcgggccggg agccaaaggc accattgatc tgaacctgcc
    12541 gctggcggat aacctgcgca atgtagcggc ggcgctcggc aaaccgttga gcgaactgac
    12601 ggtaacgatt ctggctaaac cacgccacga tgccgttatc gctgaaatgc agcaactcgg
    12661 cgtacgcgta tttgctattc cggatggcga cgttgcggcc tcaattctca cctgtatgcc
    12721 agacagcgaa gttgacgtgc tgtacggtat tggtggcgcg ccggaaggcg tagtttctgc
    12781 ggcggtgatc cgcgcattag atggcgacat gaacggtcgt ctgctggcgc gtcatgacgt
    12841 caaaggcgac aacgaagaga atcgtcgcat tggcgagcag gagctggcac gctgcaaagc
    12901 gatgggcatc gaagccggta aagtattgcg cctgggcgat atggcgcgca gcgataacgt
    12961 catcttctct gccaccggta ttaccaaagg cgatctgctg gaaggcatta gccgcaaagg
    13021 caatatcgcg actaccgaaa cgctgctgat ccgcggcaag tcacgcacca ttcgccgcat
    13081 tcagtccatc cactatctgg atcgcaaaga cccggaaatg caggtgcaca tcctctgatt
    13141 gatttgatcg attgagcctt ccagtccttc gggactggaa tttttttgtt cggagaacga
    13201 agataaggca agttaatcaa aacaggagaa aaacatggct gattgggtaa caggcaaagt
    13261 cactaaagtg cagaactgga ccgacgccct gtttagtctc accgttcacg cccccgtgct
    13321 tccgtttacc gccgggcaat tcaccaagct tggccttgaa atcgacggcg aacgcgtcca
    13381 gcgcgcctac tcctatgtta actcgcccga taatcccgat ctggagtttt acctggtcac
    13441 cgtccccgat ggcaaattaa gcccacgatt ggcggcactg aaaccaggcg atgaagtgca
    13501 ggtggttagc gaagcagctg gcttctttgt tctggatgaa gtaccggact gcgaaacgct
    13561 atggatgctg gcaaccggta cagcgattgg cccttattta tcgattctgc aactaggcaa
    13621 agatttagat cgcttcaaaa atctggtcct ggttcacgcc gcacgttatg ccgccgactt
    13681 aagctatttg ccactgatgc aagaactgga aaaacgctac gaagggaaac tgcgcattca
    13741 gacggtggtc agtcgggaaa cggcagcggg gtcgctcacc gggcggatac cggcgttaat
    13801 tgaaagtggg gaactggaaa gcgcgattgg cctgccgatg aataaagaaa ccagccatgt
    13861 gatgctgtgc ggcaatccac agatggtgcg cgatacacaa cagttgctga aagagacccg
    13921 gcagatgacg aaacatttac gtcgccgacc gggccatatg acagcggagc attactggta
    13981 agcggttact tatcgataaa cggcacgatg agcaaatccg cactcatctt attgatcatc
    14041 ccgcgatatg ccggcatcaa acggttgata aatgagtgat gatgaccaca gacaaggagg
    14101 tcgcactgct ctttttgcat aatttccagc agtgtttccg gcatttctcc gcgttcaata
    14161 cgcagttttg tcttcggcca ttgaatattt ttcgtcagtt tatacagctt gttatccgac
    14221 ttattcttca acaattgaag aatatcttct gttgcaggga agtagatacc cggatacaac
    14281 tcgcttaagc catcatcaat atgaattaac gtcaggtgag cgtcattatg tctggcgagc
    14341 tccagagctt tattcaccag taaggcatct tcttcattcc cggaaattgc cacgccaatg
    14401 tgtttataag ccatgtttaa ctccttctaa agcaactcca tcaagctaac gaacgcagtg
    14461 ataactcaaa aataatcatc tcggcctggc atgagaaagt gaaggccgca tcaagctcaa
    14521 cttgcccctc cagatcctta atcttgtagg ccacgctggc cccttcatct gcagaggatg
    14581 cagcggcggc aagctctttc agtttggtca taaaagactc tgcttcttcg cgggtggcaa
    14641 aaaagcgcga aaacttactg gtacagttgt cgttatcaat caccgtaccg atatcaatcg
    14701 cacatccttt agtactgcat ttatctacga catctttcat agggcacctc tgtatattcg
    14761 cctctctgtc gattacgact taagaataat gcgccgttaa gggaagaaca aacaagtaat
    14821 gatcttatgg attaccattt tttttgatat tgcttaaggt gaagagttca gaaggatttt
    14881 ccgggcattg tcatttgcat cgtggaaata acaatcccgg aaaaaaatag cagtaagaga
    14941 tatattaaag gttaattact tgatttattg tcggctttat acttcacatc ctgggtatct
    15001 ttaccatatt tattttcgcc ttgtgtgcca atgaacgctc caagatcgat aagcatcatc
    15061 accagaatca acgtcgggac aaaacgcccc accgcccatt gccagacacc cggtaaaatc
    15121 gcccagttac ccgccagcag catccacgcc acaatcatca gaaatgccca tgcgccggaa
    15181 cgcccgcgat catgcaagcg cttaacggtt actgccgctg ttggccaaag caagcacacc
    15241 aggcaaaacg ccgcggtctg aatatcgagt aaatttttac ccgccagtga gaaaagcacc
    15301 agcatgcctg ctaaccacag gcctatccaa atccagaaat cacggcgtcc aatacgccct
    15361 ttaaatgaga ataaccattg ctgtatggtc atgtaagttc cttgatggtt gtcttttcca
    15421 ggattctacc cttttgacaa gggcgacaga tatcgtttta atcggagcca gtcataacaa
    15481 aaggtactgt caatgaagcc agggtgtacg ctgttttttc tcttatgttc tgcattaacc
    15541 gttacaacaa cggcgcatgc acaaacacca gatacggcaa cgaccgcgcc ttatctgctg
    15601 gctggagccc ctactttcga tctctccatc agccagtttc gagaagactt taacagccag
    15661 aatcccagcc tgccactgaa cgaatttcgt gccatcgaca gcagtcccga caaagccaat
    15721 ctcactcgtg ctgcaagtaa aattaacgag aacttgtatg cttctacagc gctggagcgc
    15781 ggtaccttaa aaatcaaaag cattcaaatg acctggctac ccatccaggg gccagagcaa
    15841 aaagcagcga aagcgaaagc tcaggaatac atggcagcag tgatccgcac actcacccca
    15901 ttaatgacca aaacacaaag ccagaaaaaa ctgcagtcgc tactaacggc ggggaaaaac
    15961 aaacgttatt acaccgagac agaaggtgca ctgcgttatg ttgtcgcgga caacggcgaa
    16021 aaggggctga ccttcgctgt tgaaccgatt aagctggcgc tatctgaatc gcttgaaggt
    16081 ttgaataaat gacaaaaagc aaagcctttg tgccgatgaa tctctatact gtttcacaga
    16141 cctgctgccc tgcggggcgg ccatcttcct ttattcgctt ataagcgtgg agaattaaaa
    16201 tgcgacatcc tttagtgatg ggtaactgga aactgaacgg cagccgccac atggttcacg
    16261 agctggtttc taacctgcgt aaagagctgg caggtgttgc tggctgtgcg gttgcaatcg
    16321 caccaccgga aatgtacatc gatatggcga agcgcgaagc tgaaggcagc cacatcatgc
    16381 tgggtgcgca aaacgtggac ctgaacctgt ccggcgcatt caccggtgaa acttctgccg
    16441 ctatgctgaa agacatcggc gctcagtaca tcatcatcgg ccactctgaa cgtcgtactt
    16501 accacaaaga gtctgacgaa ctgatcgcga aaaaattcgc ggtgctgaaa gagcagggcc
    16561 tgactccggt tctgtgcatc ggtgaaaccg aagctgaaaa cgaagcgggc aaaactgaag
    16621 aagtttgcgc acgtcagatc gacgcggtac tgaaaactca gggtgctgcg gcattcgaag
    16681 gtgcggttat cgcttacgaa cctgtatggg caatcggtac tggcaaatct gcaactccgg
    16741 ctcaggcaca ggctgttcac aaattcatcc gtgaccacat cgctaaagtt gacgctaaca
    16801 tcgctgaaca agtgatcatt cagtacggcg gctctgtaaa cgcgtctaac gctgcagaac
    16861 tgtttgctca gccagatatc gacggcgcgc tggttggcgg tgcttctctg aaagctgacg
    16921 ctttcgcagt gatcgttaaa gctgcagaag cggctaaaca ggcttaagtc tgacaggtgc
    16981 cggatttcat atccggcact tactttcctt aactcttcgc cttaacgcaa aatctcacac
    17041 tgatgatcct gaatttcctc ggctgaagca cggttaagcg tcagtagatt tcgttgtgtc
    17101 gccagcaata caaatgagtt atcactctgc cgtaccatcg ccagcccgta gcttcccata
    17161 tgttcccgcg cctcaggtac ttcttctgcc agcatcataa atgggctgcg ttgtaccagt
    17221 tcgctttccg ttacccgacg cgccaggtat tcatgcccgc gcaaaccacc tggcagtggc
    17281 aaccagcggc tgctgatgtt cgccagattg ttatccagct gttcgcgcac atcaggacga
    17341 atacaagaga tatgaatatg aaaatggttt tgcgtacgcc cggtgcggga gttgatcgcc
    17401 aaagaaaccg cgcgatcggg aaccggctgg ccgtattttt tgctcataaa atcacgcgcc
    17461 tgccaggcca accaaaagaa gttcggcgtt gaaggatcgg tcaacaaagg actttcagta
    17521 ccgttaatac gatacgttgg catcaacaga tattgcagtg gaccattaag atcttttaaa
    17581 accacgtatc cggcattggg tttgacttcc gcacatggcg aaggattttg attttgctgc
    17641 tgattgggca aacattcctc aaggacaatc ttacgtaatg tatccgactc ttcaccggtt
    17701 aatttccagt aaccaatacc ggcagccaca acggcgataa ctatcatcac caaaaaaaga
    17761 agacccgctt ttttcatctt tttttccctg tacctcaaag agatgcaagg gtaacgcaaa
    17821 atcgtgacaa ataaaaaacc cggtcgcgag aaaccaccgg gtttgataat tatcctgggg
    17881 aaatcagcgt ttgctgatct gatcgaacgt accgccgtta gcaaaatgct ctttttgcgc
    17941 tttcgtccag ccgccgaact cttcatcaat ggtgaataac ttcagctttg gaaacgcatt
    18001 ttcgtacttt ttcgctactt cagcgtcgcg cggacggtag tagtttttcg cggcaatttc
    18061 ctgaccttct ggcgagtaga gatatttcag gtaggcttcc gccacctctt tggtgccttt
    18121 tttctcgacc actttatcga ccaccgacac ggttggctct gcgaggatag actcactcgg
    18181 cgtgacgatt tcgaatttat ctttccccag ttcattcgct gccagcagag cttcgttttc
    18241 ccaggcgatc agtacatcgc caatcccgcg ctcgacaaag gtgttagtgg agccgcgcgc
    18301 gccagaatcc agaacttcga cgtttttata cagtgcccga acaaaatcct gtgcttttgc
    18361 ctgatcgttg ttgttgtgat gcagcgcgta gccccaggca gccaggtagt tccagcgtgc
    18421 gccaccagag cttttcggat taggcgtgat caccgaaaca cccggtttaa tcagatcgtt
    18481 ccagtcatgg atctgcttcg gattgccctt acgcaccagg aaaacaatgg tggaagtgta
    18541 cggtgcggag ttatccggca gacgtttgat ccactcttta tcaatccgcc cgcgttccgc
    18601 aatcgcgtcc acgtcatagg ccagagccag cgtgacaaca tcagcttcaa taccgttgat
    18661 taccgacgtc gcttgtttac ctgaaccacc gtgtgactga cgaatcacca cgttatcacc
    18721 agtttgctgt ttccagtggg cgctgaatgc cttgttgtac tgttcgtaca attcgcgcgt
    18781 tggatcatat gaaacgttaa gaagctgaat atcctttgcc ataacgctgg ttgccgccag
    18841 caaaaatgtt aaccctacgc cccacttgtt catcgcccga ctctcttatg ttgtgttgtg
    18901 atgagcaaag cgtgccagaa ggttaaccaa acattaaaga ataaaaaaag attggctata
    18961 acttgcgggt atatgttgag ggattaaaaa ggcggaagaa atccgcctca tattgctgac
    19021 aaagtgcgct ttatcgatgc cggatgcggc gcgaacgcct tatccgacct acaaaaattt
    19081 gtaaattcaa taaattgcag aattcacgta ggcctgataa gcgaagcgca tcaggcattt
    19141 ttgcttctgt catcggtttc aggctaaagg aatctgcctt tttccgaaat cattaataca
    19201 gttttttcgc gcagtccagc cagtcacctt tgaacggacg cttcatgttt tcgatagcgt
    19261 cgatgatgtc gtggtgaacc agctgttcgt tctggatacc tacacaacga ccgccgtaac
    19321 ctgccagcag cagatcgata gcgtaagcgc ccatacggga agccagaata cggtcgtaag
    19381 gcaccggaga accaccgcgc tggatgtggc ccagcacagt tgcgcgggtt tcacgaccgg
    19441 tttctttctc gatgaaatgc gccagttcgt caacatcaca catatgttcg gtaatcgcca
    19501 cgatcgcgtg ttttttacct ttcgcgatac ccgctttgat ttcgtttacc aggtcttcac
    19561 ggctgaattc aacttccgga actacaacga attcacagcc accggcaatg gccgcagcca
    19621 gggtcagatc gccacaataa cgacccatca cttccaccac ggaaatacgc tggtgagaag
    19681 aagaggtgtc acgcagacgg tcgatcgctt ctacaacggt gctcagcgca gtgaagaaac
    19741 cgatagtgta gtcagtgcct ttgatgtcgt tgtcgatagt gcccggcaga ccgatgcacg
    19801 ggaagcccat ttcggtcaga cgcattgcac ccatgtagga accgtcaccg ccgataacca
    19861 ccagcgcgtc gataccacgt tttttcaggt tttcgatagc cacggcgcgg atgttctcgt
    19921 cgcggaattc cgggaagcgc gcagaaccga ggaacgtacc gccacggttg atcatgtcag
    19981 aaacgctgta acggtctagc tgtaccatac ggtcttcata cagacccaga tagccgtcat
    20041 aaatgcccat tacttccaga ccttctgtca gcgcagaacg aacaaccccg cgaattgcgg
    20101 cgttcatgcc tggcgcatca ccgccgcttg tcaacacacc gattttctta atcatgacta
    20161 cctctgaact ttggaatgca aaataaaatc tgttgccgga agtcttcttg cacatcgaag
    20221 tgatccaacg aatgtgcaaa tagtataaca atcacttcct gctgaattga ttcaggtcag
    20281 gccaaatggc ggtattttat acacaaaatg cgggtctggc tctcttttat actgattatg
    20341 aaagcataga ccgtttaccc tccctgggta cgacggaaca ggggtcctga tggataatta
    20401 catccgatcc cggaaaacgc cgtaaaatag cctgctctac ctgatccgcc accatatgtg
    20461 cctgaaccaa aggcagagag tcttccattt ccaaatgaat ctgaataaag cgggtcggcc
    20521 ctgactgccg cgtgcgaaga tcgtgagcgc cgctaacacc cggccaggaa gtcacgatat
    20581 caataatttc ttgccgttcc tcatcaggca atgcgcgatc cagtaatgac tgtaccgcct
    20641 catatcccat gcgtaacgcg ctatataaaa tatagatgcc gattcccaat gcaaacagag
    20701 catcggcgcg atgccagccg taccaggaca accccagcgc cagcagaatt gcgccgttca
    20761 tcataacatc agactggtaa tgtagcatat cagcccgcac cgcctggctt tgcgtccggc
    20821 gcaccaccca acgctgaaac gagacaagga taatcgtaca aattagcgcc acaattgtca
    20881 cgataacccc gacgcctgga tctgtcatcg gtgttggaga tatcagatgt tgaatacccg
    20941 tcaaaaacag gaatagtgcc gaaccggaga taaacatact ttgcgccagc gccgcgaggg
    21001 actctgcttt accgtgacca aacgagtgat tatcgtcggc aggttgcagg gaatatcgca
    21061 ccaccagtaa attcgtcaac gacgcgccga tatccaccag cgaatccacc agcgcggcga
    21121 gaatactcac cgacccggta taccaccatg caaaaatttt aatcagcaat agcaacgaag
    21181 ccatcgccgt cgcagcaatc gccgcccgac tgaccagccg tccataagat tgattcataa
    21241 atactcccgc tatcaactga cgctagtata acggaagcaa atcatctgca atgcattaag
    21301 cagcaggcaa attgaggata aaaaaaaccc ccacatcatg tgggggaaga cagggatggt
    21361 gtctatggca aggaaaacag ggtttactac tgggaacgtg agttgctact actcaatagc
    21421 ttcaacgatg aacttttttg ccattgcgtc acgtcacgca actgctccat tcgttgttga
    21481 tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg
    21541 acttttgcca tctcaacctg acgagcaatt tgctcattcg ccattttttc tgcctgtgcg
    21601 cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc gatgcattgt ctccagttcg
    21661 ctaacattaa caggaggctg ttcgtgccgg gcctgttgca taagatctcg catctgctga
    21721 cgctgatgtt cggttaaact tatgccgtcg aacatatggc tctgcgtact gcgctgcgta
    21781 agttcttcac ccggatgcca gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa
    21841 ctgactgcca gcgttgaggc catgacggca gcggtaacta tgcgcatcat ttgctcccaa
    21901 aatctttctg tcgcgattca acgatagaga gtttacgatt caggctgcaa acatgcgtca
    21961 gggggtgtaa aacaacgtaa agtcatggat tagcgacgtc tgatgacgta atttctgcct
    22021 cggaggtatt taaacaatga ataaaatcct gttagttgat gatgaccgag agctgacttc
    22081 cctattaaag gagctgctcg agatggaagg cttcaacgtg attgttgccc acgatgggga
    22141 acaggcgctt gatcttctgg acgacagcat tgatttactt ttgcttgacg taatgatgcc
    22201 gaagaaaaat ggtatcgaca cattaaaagc acttcgccag acacaccaga cgcctgtcat
    22261 tatgttgacg gcgcgcggca gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga
    22321 tgactatctc ccgaaaccgt ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct
    22381 gcgccgttcg cactggagcg agcaacagca aaacaacgac aacggttcac cgacactgga
    22441 agttgatgcc ttagtgctga atccaggccg tcaggaagcc agcttcgacg ggcaaacgct
    22501 ggagttaacc ggtactgagt ttaccctgct ctatttgctg gcacagcatc tgggtcaggt
    22561 ggtttcccgt gaacatttaa gccaggaagt gttgggcaaa cgcctgacgc ctttcgaccg
    22621 cgctattgat atgcacattt ccaacctgcg tcgtaaactg ccggatcgta aagatggtca
    22681 cccgtggttt aaaaccttgc gtggtcgcgg ctatctgatg gtttctgctt catgataggc
    22741 agcttaaccg cgcgcatctt cgccatcttc tggctgacgc tggcgctggt gttgatgttg
    22801 gttttgatgt tacccaagct cgattcacgc cagatgaccg agcttctgga tagcgaacag
    22861 cgtcaggggc tgatgattga gcagcatgtc gaagcggagc tggcgaacga tccgcccaac
    22921 gatttaatgt ggtggcggcg tctgttccgg gcgattgata agtgggcacc gccaggacag
    22981 cgtttgttat tggtgaccac cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag
    23041 atcattcgta actttattgg tcaggccgat aacgccgatc atccgcagaa gaaaaagtat
    23101 ggccgcgtgg aactggtcgg tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt
    23161 tatctgattc gtccggccag cagttctcaa tccgatttca ttaacttact gtttgaccgc
    23221 ccgctattac tgctgattgt caccatgttg gtcagtacgc cgctgctgtt gtggttggcc
    23281 tggagtctgg caaaaccggc gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga
    23341 aacttacgcc agcacccgga actggaagcg gggccacagg aattccttgc cgcaggtgcc
    23401 agttttaacc agatggtcac cgcgctggag cgcatgatga cctctcagca gcgtctgctt
    23461 tctgatatct ctcacgagct gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta
    23521 ctgcgccgtc gtagtggtga aagcaaggaa ctggagcgta ttgaaaccga agcgcaacgt
    23581 ctggacagca tgatcaacga tctgttggtg atgtcccgta atcagcaaaa aaacgcgctg
    23641 gttagcgaaa ccatcaaagc caaccagttg tggagtgaag tgctggataa cgcggcgttc
    23701 gaagccgagc agatgggcaa gtcgttgacg gttaacttcc cgcctgggcc gtggccgctg
    23761 tacggcaatc cgaacgcact ggaaagtgcg ctggaaaaca ttgttcgtaa tgctctgcgt
    23821 tattcccata cgaagattga agtgggcttt gcggtagata aagacggtat caccattacg
    23881 gtggacgacg atggtcctgg cgttagcccg gaagatcgcg aacagatttt ccgtccgttc
    23941 tatcggaccg atgaagcacg cgatcgtgaa tctggcggta caggattggg gctggcgatt
    24001 gttgaaaccg ccattcagca gcatcgtggc tgggtgaagg cagaagacag cccgctgggc
    24061 ggtttacggc tggtgatttg gttgccgctg tataagcgga gttaaactcc gcatttgtag
    24121 gcaggataag gcgtttacgc cgcatccggc atttgagcag gatgcctgat gcgacgctga
    24181 tagcgtctta tcaggcctac actccggggg ttattttccc cacaaccggc gggaaaaatc
    24241 ttcgatcttg ccgctcagtc ggcgcttttg catcgtccgc gtccagcttt tcgataaccc
    24301 ggcggcggag agtaaacggt gatattgatc gtcatcaaac ggcatatgcc atgcgatggc
    24361 ggcagcctcc tgcacggtca catcgctgac acgggaaacc aactccagcg gcgcgtccgc
    24421 agacactttc cctggcgcta tcacgctata caaccacccc accttacctg tgttttgcat
    24481 caactgcgct atatcgctga tatcaaaatg gtaattgagt ttatagcaag gcgagcgcgg
    24541 ctggctgacc tgaattaatg cctctcccca gcggaaaata tcgcccatat aaacattact
    24601 ttccgtgaga ccgtcggttg agagattttc gccaaacgca ggcgcaacaa acaactccgc
    24661 ctgttccgga aattcccgcg cccagtagag ataatgctca cgaggataat gacacagcgc
    24721 tctgtctggc ccgccgtgaa cctttttctc cgcctgctcg tcaccttcca gccccagctc
    24781 cgtcagcatc aactcaccat caacctggat tttggcaatt gcgctgggtt tgccttcggg
    24841 ataagcctga atcttgcctg tgtatacatc aaccggatat cgcatctgtt ccctctccac
    24901 gagaaatttc cccattagac catatttatt ttatgtcagg cgtgctgttt tcgcggtaag
    24961 tatccaggca ggaactcaat agcaaaccgg acgaacgtta gttcatccgg ctgtggggat
    25021 taaggaagca ggtttatttc accgtaccta aaatttcgat tttcgctgct ctggctttga
    25081 ctttacgtga ccagatagag gtaagaattg gcaccagaat cgaagtcaca atgaccgccg
    25141 tcgctaccag cgaagttgct gccggagcca tcggtttaaa cgcaggcacc atttctgcaa
    25201 tcagcacagg tgtcgctacc gcggcccctg cggaactgga agcggcaatt ccggcagtgc
    25261 catcgccacc gccaatcaat ttatcggcga taatcaacgg aataccggtc acgataatta
    25321 ctgccacacc caacaggatc cccagcaaac cagtctgggc aattacagtc aaatcaatgg
    25381 tattgcccag tgcgaaggca aagaatggaa tcagcgtctg caccgctttg ctgaaaaatt
    25441 ctcgtaattc agggtcaagg ttccccaggg caaagcccac caggaacggt aatactgcgc
    25501 cgacgaaaac atgcggttca aacgaggcaa tccctgcagt gcccagaata atcatcgtca
    25561 tgagcggccc ggactccaac gacatcaaca caaatgcccc agcttcttct tttgtgccgt
    25621 actgctgcat gatggaagcg taaagtccgc cattggtcat atccatcgcc gccaccagcg
    25681 ccagcgttga aagtccggca aagaatccaa cttcaacacc atgttccgga ataatgcgcg
    25741 aggcaattgc cgcaaccacc cacgcgacgg caatttttgt taccaccagc gtaccggatt
    25801 tacgcaatac cgttcccgtc gcgcttaatt ttattgacgc ccccatgcaa aaaaaccaca
    25861 ccgccagaat gggcaccgta ccggtaatca tcccgttggt gaatgatcca aaatatttcc
    25921 ccgcccccgg cgagaaggtg tggcacagtg cgccaaggaa tagcgggacg agcatcatcc
    25981 cccccgggat tttctcaatc gagcgtttta tctgcatttc catcacctgt cggcatattt
    26041 ttaagggctg atgcgtcatt tctcaatgga gcatcagtgt tgatggaaac gaagatagac
    26101 attcatttaa tcaataaaag tgatcaaaac cacatcaatt gaaacactgt tttattttta
    26161 tcggatcatt gtttttattt tgaaattgat cacaaaaaaa ccaccgccgt tggcgatggt
    26221 tcatttttta agctgatatg cggcctatac gcctcattgc agcaggcggc aaatgattat
    26281 tttttcgccg caaaacgtgc cgctgcttcg tcccagttca ccacgttcca gaactcttta
    26341 atgtagtccg gacggcggtt ctggaatttc aggtagtaag catgttccca cacatccagg
    26401 cccagaatcg ggaagccgga agcgccagaa atagcttcac ccatcagcgg agaatcctgg
    26461 ttagcagtag aaaccaccgc cagtttatcg cctttcagca ccagccatgc ccagccggaa
    26521 ccaaagcggg aagctgccgc tttttcaaat tctgctttga agttatcaac ggagccgaag
    26581 tcacgttcga tagccgcttt caggtcaccc tgcagggtgg tgcctttttt cagacctttc
    26641 cagaacaggc tgtggttagc gtgaccgcca gcgttgtttc gcagtacggt tttcttgtct
    26701 gctggcagct ggtccagttt ggtgatcagc tcttcaaccg gcaggttggc aaattctggc
    26761 aggctttcca gcgccgcgtt ggcgttgttg acgtaggtct gatggtgttt ggtgtggtgg
    26821 atttccatgg tctgcttatc gaagtgcggt tccagggcat cgtaagcata cggcagggat
    26881 ggcagggtat agctcatatt catctccagt attgtcgggc ggccgattgt taatgccgcg
    26941 taagcagttg gttcattata gttaattaaa tgatattgaa aatgattatc aatgccgtac
    27001 ttttcgtaag ggtatggttt tgcaggaaaa tgcccgagat gtgaagcaaa tcacccactt
    27061 aatgccgtga ttgccagtaa atcgacaacg gcggcaacag gcgaaaggtt aatcgacagc
    27121 acgattttta cactcatctc gtcggagatg tgacgcgacg aaaaatgatg aggataagaa
    27181 gatgagtaac gcgattacga tggggatatt ttggcatttg atcggcgcgg ccagtgcagc
    27241 ctgtttttac gctccgttca aaaaagtaaa aaaatggtca tgggaaacca tgtggtcagt
    27301 cggtgggatt gtttcgtgga ttattctgcc gtgggccatc agcgccctgt tactaccgaa
    27361 tttctgggcg tattacagct cgtttagtct ctctacgctg ctgcctgttt ttctgttcgg
    27421 cgctatgtgg gggatcggta atatcaacta tggcctgacc atgcgttatc tcggcatgtc
    27481 gatgggaatt ggcatcgcca ttggcattac gttgattgtc ggtacgctga tgacgccaat
    27541 tatcaacggc aatttcgatg tgctgattaa caccgaaggc ggacgcatga cgttgctcgg
    27601 cgttctggtg gcgctgattg gcgtagggat tgtaactcga gccgggcagt tgaaagagcg
    27661 caagatgggc attaaagccg aagagttcaa tctgaaaaaa gggctggtgc tggcggtgat
    27721 gtgcggcatt ttctctgccg ggatgtcctt tgcgatgaac gccgcaaaac cgatgcatga
    27781 agccgctgcc gcactaggcg tcgatccact gtatgtcgct ctgccaagct atgtcatcat
    27841 catgggcggc ggcgcgatca tcaacctcgg tttctgtttt attcgtctgg caaaagtgaa
    27901 ggatttgtcg ctaaaagccg acttctcgct ggcaaaaccg ctgatcactc acaatgtgtt
    27961 actctcagca ctgggcgggt tgatgtggta tctgcaattc tttttctatg cctggggcca
    28021 cgcccgcatt ccggcgcaat atgactacat cagttggatg ctgcatatga gcttctatgt
    28081 attgtgcggc ggtatcgtcg ggctggtgct gaaagagtgg aacaatgcag gacgccgtcc
    28141 ggtaacggtg ttgagcctcg gttgtgtggt gattattgtc gccgctaaca tcgtcggcat
    28201 cggcatggcg aattaatctt tctgcgaatt gagatgacgc cactggctgg gcgtcatccc
    28261 ggtttcccgg gtaaacacca ccgaaaaata gttactatct tcaaagccac attcggtcga
    28321 aatatcactg attaacaggc ggctatgctg gagaagatat tgcgcatgac acactctgac
    28381 ctgtcgcaga tattgattga tggtcattcc agtctgctgg cgaaattgct gacgcaaaac
    28441 gcgctcactg cacgatgcct catcacaaaa tttatccagc gcaaagggac ttttcaggct
    28501 agccgccagc cgggtaatca gcttatccag caacgtttcg ctggatgttg gcggcaacga
    28561 atcactggtg taacgatggc gattcagcaa catcaccaac tgcccgaaca gcaactcagc
    28621 catttcgtta gcaaacggca catgctgact actttcatgc tcaagctgac cgataacctg
    28681 ccgcgcctgc gccatcccca cgctacctaa gcgccagtgt ggttgccctg cgctggcgct
    28741 aaatcccgga atcgccccct gccagtcaag attcagcttc agacgctccg ggcaataaat
    28801 aatattctgc aaaaccagat cgttaacgga agcgtaggag tgtttatcgt cagcatgaat
    28861 gtaaaagaga tcgccacggg taatgcgata agggcgatcg ttgagtacat gcaggccatt
    28921 accgcgccag acaatcacca gctcacaaaa atcatgtgta tgttcagcaa agacatcttg
    28981 cggataacgg tcagccacag cgactgcctg ctggtcgctg gcaaaaaaat catctttgag
    29041 aagttttaac tgatgcgcca ccgtggctac ctcggccaga gaacgaagtt gattattcgc
    29101 aatatggcgt acaaatacgt tgagaagatt cgcgttattg cagaaaacca tcccgtccct
    29161 gacgaatatc acgcggtgac cagttaaact ctcggcgaaa aagcgtcgaa aagtggttac
    29221 tgtcgctgaa tccacagcga taggcgatgt cagtaacgct ggcctcactg tggcgtagca
    29281 gatgtcgggc tttcatcagt cgcaggcggt tcaggtatcg ctgaggcgtc agtcccgttt
    29341 gctgcttaag ctgccgatgt agcgtacgca gtgaaagaga aaattgatcc gccacggcat
    29401 cccaattcac ctcatcggca aaatggtcct ccagccaggc cagaagcaag ttgagacgtg
    29461 atgcgctgtt ttccaggttc tcctgcaaac tgcttttacg cagcaagagc agtaattgca
    29521 taaacaagat ctcgcgactg gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca
    29581 tctgtgcaac cagctgtcgc acctgctgca atacgctgtg gttaacgcgc cagtgagacg
    29641 gatactgccc atccagctct tgtggcagca actgattcag cccggcgaga aactgaaatc
    29701 gatccggcga gcgatacagc acattggtca gacacagatt atcggtatgt tcatacagat
    29761 gccgatcatg atcgcgtacg aaacagaccg tgccaccggt gatggtatag ggctgcccat
    29821 taaacacatg aatacccgtg ccatgttcga caatcacaat ttcatgaaaa tcatgatgat
    29881 gttcaggaaa atccgcctgc gggagccggg gttctatcgc cacggacgcg ttaccagacg
    29941 gaaaaaaatc cacactatgt aatacggtca tactggcctc ctgatgtcgt caacacggcg
    30001 aaatagtaat cacgaggtca ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa
    30061 acgtcgattt ttcaagatac agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc
    30121 acaattcagc aaattgtgaa catcatcacg ttcatctttc cctggttgcc aatggcccat
    30181 tttcctgtca gtaacgagaa ggtcacgaat tcaggcgctt tttagactgg tcgtaatgaa
    30241 attcagcagg atcacattat gacctttcgc aattgtgtcg ccgtcgatct cggcgcatcc
    30301 agtgggcgcg tgatgctggc gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa
    30361 atccatcgtt ttaaaaatgg gctgcatagc cagaacggtt atgtcacctg gaatgtggat
    30421 agcctggaaa gtgccattcg ccttggatta aacaaggtgt gcgaggaagg gattcgtatc
    30481 gatagcattg ggattgatac ctggggcgtg gactttgtgc tgctcgacca acagggtcag
    30541 cgtgtgggcc tgcccgttgc ttatcgcgat agccgcacca atggcctaat ggcgcaggca
    30601 cagcaacaac tcggcaaacg cgatatttat caacgtagcg gcatccagtt tctgcccttc
    30661 aatacgcttt atcaactgcg tgcgctgacg gagcaacaac ctgaacttat tccacacatt
    30721 gctcacgctc tgctgatgcc ggattacttc agctatcgcc tgaccggcaa gatgaactgg
    30781 gagtacacca atgccaccac cacacaactg gtcaatatca atagcgacga ctgggacgag
    30841 tcgctactgg cgtggagcgg ggccaacaaa gcctggtttg gtcgcccgac gcatccgggt
    30901 aatgtcattg ggcactggat ttgcccgcag ggtaatgaga ttccggtggt cgccgttgcc
    30961 agccatgata ccgccagcgc ggttatcgcc tcgccgttaa acggttcacg cgccgcttat
    31021 ctctcttctg gcacctggtc attgatgggc ttcgaaagcc agacgccatt taccaatgac
    31081 acggcgctgg cagccaacat caccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg
    31141 aaaaatatta tgggcttatg gctgcttcag cgagtgcttc aggaacggca aatcaacgat
    31201 ctcccggcgc ttatcgccgc gacacaggca cttccggcct gtcgcttcat catcaatccc
    31261 aatgacgatc gctttattaa tcctgaagcg atgtgcagcg aaattcaggc tgcgtgtcgg
    31321 gaaacggcgc aaccgatccc ggaaagtgat gctgaactgg cgcgctgtat tttcgacagt
    31381 ctggcgctgc tgtatgccga tgtgttgcat gagctggcgc agctgcgcgg tgaagatttc
    31441 tcgcaactgc atattgtcgg cggcggctgc cagaacacgc tgctcaacca gctatgcgcc
    31501 gatgcctgcg gtattcgggt gatcgccggg ccagttgaag cctcgacgct cggcaatatc
    31561 ggcatccagt taatgacgct ggatgaactc aacaatgtgg atgatttccg tcaggtcgtc
    31621 agcaccaccg cgaatctgac cacctttacc cctaatcctg acagtgaaat tgcccactat
    31681 gtggcgcaga ttcactctac acgacagaca aaggagcttt gcgcatgacc actcaactgg
    31741 aacaggcctg ggagctggcg aaacagcgtt tcgcggcggt ggggattgat gtcgaggagg
    31801 cgctgcgcca acttgatcgt ttacccgttt caatgcactg ttggcagggc gatgatgttt
    31861 ccggttttga aaacccggaa ggttcgctga ccggtgggat tcaagctact ggtaattatc
    31921 cgggcaaagc gcgtaatgcc agtgagctac gtaccgatct ggaacaggct atgcggctga
    31981 ttccggggcc gaaacggctt aatttacatg ccatctatct ggaatcagat acgccagtct
    32041 cgcgcgatca gatcaaacca gagcacttca aaaactgggt tgaatgggcg aaagccaatc
    32101 agctcggtct ggattttaac ccctcctgct tttcgcatcc gctaagcgcc gatggcttta
    32161 cgctttccca tgccgacgac agcattcgcc agttctggat tgatcactgc aaagccagcc
    32221 gtcgcgtttc ggcctatttt ggcgagcaac tcggcacacc atcggtgatg aacatctgga
    32281 tcccggatgg tatgaaagat atcaccgttg accgtctcgc cccgcgtcag cgtctgctgg
    32341 cagcactgga tgaggtgatc agcgagaagc taaaccctgc gcaccatatc gacgccgttg
    32401 agagcaaatt gtttggcatt ggcgcagaga gctacacggt tggctccaat gagttttaca
    32461 tggggtatgc caccagccgc cagactgcgc tgtgcctgga cgccgggcac ttccacccga
    32521 ctgaagtgat ttccgacaag atttccgccg ccatgctgta tgtgccgcag ttgctgctgc
    32581 acgtcagccg tccggttcgc tgggacagcg atcacgtagt gctgctggat gatgaaaccc
    32641 aggcaattgc cagtgagatt gtgcgtcacg atctgtttga ccgggtgcat atcggccttg
    32701 acttcttcga tgcctctatc aaccgcattg ccgcgtgggt cattggtaca cgcaatatga
    32761 aaaaagccct gctgcgtgcg ttgctggaac ctaccgccga gctgcgcaag ttggaagcgg
    32821 cgggcgatta cactgcgcgt ctggcactgc tggaagagca gaaatcgttg ccgtggcagg
    32881 cggtctggga aatgtattgc caacgtcacg ataccccaac gggtagcgaa tggctggaga
    32941 gcgtgcgggc ttatgagaaa gcgattttga gccagcgtgg gtaaacactg ccggatgcgg
    33001 cacgagcgcc ttatccggcc tacgggtcga atgtccatgc gggctgtcac cctcaccccg
    33061 gccctctccc tgaaagggcg agggagaaaa acatagcctc gtgcggcaac ggagctgaac
    33121 actgctccgg acagtcccct cgcccctacg gggagagggt tagggtgagg ggaacaagcc
    33181 gcaccatgtc taaacaaggc aacctaattt acaggaacac agaacatgca aaacattacc
    33241 cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg atgcctggct gaaaggctgg
    33301 gacgagcgca acggcggcaa cctgacgctg cgcctggatg acgccgatat cgcgccttat
    33361 aaagacaact tccatgcaca accgcgctat atcccgctca gccagcccat gcctttactg
    33421 gcaaatacac cgtttattgt caccggctcc ggcaaattct tccgtaacgt ccagcttgat
    33481 cctgcagcta acttaggcgt cgtaaaagtc gacagcgacg gcgcgggcta ccatattctc
    33541 tgggggttaa ccaacgaagc cgttccaact tctgaacttc cggctcactt tctctcccac
    33601 tgcgagcgca ttaaagccac caacggtaaa gatcgggtga tcatgcactg ccacgccacc
    33661 aacctgatcg ccctcaccta tgtactggaa aacgacaccg cggtctttac tcgccaactg
    33721 tgggaaggca gcaccgagtg tctggtggta ttcccggatg gcgttggcat tctgccgtgg
    33781 atggtgcccg gcacggacga aatcggccag gcgaccgcac aagagatgca aaaacattcg
    33841 ctggtgttgt ggcccttcca cggcgtcttc ggcagcgggt cgacgttgga tgaaacattc
    33901 ggtttaatcg acaccgcaga aaaatcagca caagtattag tgaaggttta ttcgatgggc
    33961 ggcatgaaac agaccatcag ccgtgaagag ttgatagcgc tcggcaagcg cttcggcgtt
    34021 acaccactag ccagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgtc
    34081 atgcaggtta accccgacgc tcacgaagag tatcagcgtc ggcataaccc catctggccg
    34141 gaactggaag cagtgttgaa atctcacggt gcgcataact acgccattta cctcgacaaa
    34201 gcgcgtaatc tgctgtttgc cacggtagag attgaatctg aagagcgctg gaatgcggtt
    34261 gccagtactg atgtttgcca acgttggtgg aaatatatga ccgacgtcat gcccgcgaac
    34321 ccggataaca gcccggtgag tagcgaactg caagaagtgt tttacctgcc gtaattgcct
    34381 aatgtgccgg agacgcctcc ggcacaatat tttta
//
LOCUS       DAPGWD010000034        34371 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPGWD010000034 DAPGWD010000000
VERSION     DAPGWD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.034"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            39..623
                     /locus_tag="RHX88_001605"
     CDS             39..623
                     /locus_tag="RHX88_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2230304.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            703..1398
                     /locus_tag="RHX88_001606"
     CDS             703..1398
                     /locus_tag="RHX88_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2230305.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1428..3944
                     /locus_tag="RHX88_001607"
     CDS             1428..3944
                     /locus_tag="RHX88_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2230306.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDAKGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3955..5046
                     /locus_tag="RHX88_001608"
     CDS             3955..5046
                     /locus_tag="RHX88_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2230307.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFNITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5089..5949)
                     /gene="rsmI"
                     /locus_tag="RHX88_001609"
     CDS             complement(5089..5949)
                     /gene="rsmI"
                     /locus_tag="RHX88_001609"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDV2230308.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6013..8049
                     /gene="lpoA"
                     /locus_tag="RHX88_001610"
     CDS             6013..8049
                     /gene="lpoA"
                     /locus_tag="RHX88_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDV2230309.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8007..8402
                     /locus_tag="RHX88_001611"
     CDS             8007..8402
                     /locus_tag="RHX88_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDV2230310.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8422..9012
                     /gene="diaA"
                     /locus_tag="RHX88_001612"
     CDS             8422..9012
                     /gene="diaA"
                     /locus_tag="RHX88_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDV2230311.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9022..9597
                     /gene="yraP"
                     /locus_tag="RHX88_001613"
     CDS             9022..9597
                     /gene="yraP"
                     /locus_tag="RHX88_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDV2230312.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9711..10751)
                     /locus_tag="RHX88_001614"
     CDS             complement(9711..10751)
                     /locus_tag="RHX88_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV2230313.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10824..11459)
                     /locus_tag="RHX88_001615"
     CDS             complement(10824..11459)
                     /locus_tag="RHX88_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDV2230314.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVASAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11587..12105
                     /gene="yhbO"
                     /locus_tag="RHX88_001616"
     CDS             11587..12105
                     /gene="yhbO"
                     /locus_tag="RHX88_001616"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDV2230315.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12085..12528)
                     /locus_tag="RHX88_001617"
     CDS             complement(12085..12528)
                     /locus_tag="RHX88_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDV2230316.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12579..12881
                     /gene="yhbQ"
                     /locus_tag="RHX88_001618"
     CDS             12579..12881
                     /gene="yhbQ"
                     /locus_tag="RHX88_001618"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDV2230317.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12868..13371)
                     /locus_tag="RHX88_001619"
     CDS             complement(12868..13371)
                     /locus_tag="RHX88_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2230318.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13365..13889)
                     /locus_tag="RHX88_001620"
     CDS             complement(13365..13889)
                     /locus_tag="RHX88_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDV2230319.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14098..15093
                     /locus_tag="RHX88_001621"
     CDS             14098..15093
                     /locus_tag="RHX88_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV2230320.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15102..15980
                     /locus_tag="RHX88_001622"
     CDS             15102..15980
                     /locus_tag="RHX88_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV2230321.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16186..17193
                     /locus_tag="RHX88_001623"
     CDS             16186..17193
                     /locus_tag="RHX88_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDV2230322.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSRSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFRALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17311..18555)
                     /gene="mtr"
                     /locus_tag="RHX88_001624"
     CDS             complement(17311..18555)
                     /gene="mtr"
                     /locus_tag="RHX88_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDV2230323.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFVPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18709..20598)
                     /gene="deaD"
                     /locus_tag="RHX88_001625"
     CDS             complement(18709..20598)
                     /gene="deaD"
                     /locus_tag="RHX88_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDV2230324.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20591..20671)
                     /gene="yrbN"
                     /locus_tag="RHX88_001626"
     CDS             complement(20591..20671)
                     /gene="yrbN"
                     /locus_tag="RHX88_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDV2230325.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20778..21662)
                     /gene="nlpI"
                     /locus_tag="RHX88_001627"
     CDS             complement(20778..21662)
                     /gene="nlpI"
                     /locus_tag="RHX88_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDV2230326.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21771..23906)
                     /gene="pnp"
                     /locus_tag="RHX88_001628"
     CDS             complement(21771..23906)
                     /gene="pnp"
                     /locus_tag="RHX88_001628"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDV2230327.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24153..24422)
                     /gene="rpsO"
                     /locus_tag="RHX88_001629"
     CDS             complement(24153..24422)
                     /gene="rpsO"
                     /locus_tag="RHX88_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDV2230328.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24571..25515)
                     /gene="truB"
                     /locus_tag="RHX88_001630"
     CDS             complement(24571..25515)
                     /gene="truB"
                     /locus_tag="RHX88_001630"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDV2230329.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25515..25916)
                     /gene="rbfA"
                     /locus_tag="RHX88_001631"
     CDS             complement(25515..25916)
                     /gene="rbfA"
                     /locus_tag="RHX88_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDV2230330.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26080..28752)
                     /gene="infB"
                     /locus_tag="RHX88_001632"
     CDS             complement(26080..28752)
                     /gene="infB"
                     /locus_tag="RHX88_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDV2230331.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28777..30264)
                     /gene="nusA"
                     /locus_tag="RHX88_001633"
     CDS             complement(28777..30264)
                     /gene="nusA"
                     /locus_tag="RHX88_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDV2230332.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30292..30744)
                     /gene="rimP"
                     /locus_tag="RHX88_001634"
     CDS             complement(30292..30744)
                     /gene="rimP"
                     /locus_tag="RHX88_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDV2230333.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30951..31027)
                     /locus_tag="RHX88_001635"
     tRNA            complement(30951..31027)
                     /locus_tag="RHX88_001635"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30991..30993),aa:Met,seq:cat)
     gene            31375..32718
                     /gene="argG"
                     /locus_tag="RHX88_001636"
     CDS             31375..32718
                     /gene="argG"
                     /locus_tag="RHX88_001636"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDV2230334.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(32726..34351)
                     /locus_tag="RHX88_001637"
     CDS             complement(32726..34351)
                     /locus_tag="RHX88_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV2230335.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVVRTTENDIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT         8499 a         9205 c         8496 g         8171 t
ORIGIN      
        1 aatatgatta tgtacttgtt acaaggataa ggttataaat gaataaagtt acaaaaacag
       61 ctattgtggg cttacttgcg cttttcgcag gtaatgccgc tgcaaccgat ggtgaaatag
      121 tttttgatgg cgagatttta aaatccgctt gtgaaatcaa tgactctgat aagaaaattg
      181 aagttgctct tggtcactat aatgccgaac agtttcgttc tgttggcgat cgtagcccaa
      241 aaattccatt cactattcct ttagttaact gcccagtgac tgggtgggag cacgataacg
      301 gcaacgttga agcgtctttc cgtttatggc tggaaacgcg ggataatggc accgtcccta
      361 acttccctaa tctggcaaaa gtgggctctt ttgctggcac agcggcaaca ggtgtgggta
      421 tccgtattga cgatgcggaa agcggaaacc ttatgccact gaatgctatg gggaatgata
      481 acacggttta tcagatccca gcggactcca atggtattgt caatgttgac ctcatcgctt
      541 actacgtatc aaccgttgaa gcatcagaaa tcaccccagg ggaagcagac gctgtcgtta
      601 acgtaacgct ggattaccgt taatacgtta cggcgttatc tgacctttca gataacgccc
      661 ttttccttcc tctttctcgt tgtatcaggt tgaaaaataa ctatgtcaaa acgaacattc
      721 gcggtgataa taaccttgtt gtgtagcttc tgtatcggcc aggcgcttgc agggggtatc
      781 gttttacagc gaacgcgagt gatctatgat gccagccgca aagaggctgc gttacctgtc
      841 gcaaacaaag gcgcagaaac gccttattta ctgcaatcat gggtagataa tatagatggt
      901 acaagccgtg ccccatttat tataacccca ccgctatttc gtcttgaagc tggcgatgac
      961 tcatcactgc gaattattaa aacagctgat aacctgcctg aaaataaaga gtcgctgttc
     1021 tacattaatg ttcgtgccat tccagcaaag aaaaaatcag atgatgttaa tgctaacgag
     1081 ttgacgctgg tatttaaaac acggatcaaa atgttttatc gccccgcaca cctgaaggga
     1141 cgggtaaacg atgcgtggac atcactggaa tttaaacgta gtgaccattc actcaatata
     1201 tataacccaa ctgaatatta cgtcgtattt gccggactgg cagtcgataa aaccgatctc
     1261 acaagcaaaa ttgaatatat cgcgcccgga gaacataaac agttaccact tcctgcatct
     1321 ggcggaaaga acgtgaaatg ggctgcaatc aatgattatg gcggcagttc cgggacagaa
     1381 actcgtccac tgcaataaca aatataaaaa acacaggtca tcagggaatg ccacaacgac
     1441 accaccaggg acataaacgc acaccgaaac agttggcgct catcatcaaa cgctgtttgc
     1501 cgatggtgct cactggcagc ggcatgcttt gcactaccgc taacgccgaa gagtattatt
     1561 tcgaccccat tatgctggaa actacaaaaa gtggtatgca aacaactgat ctgtcacgtt
     1621 tttcaaaaaa atacgcacaa ctaccaggaa cttatcaggt tgatatctgg ctgaataaaa
     1681 agaaggtttc acagaaaaaa attacattta ccgccaatgc agagcaactt ctgcagccac
     1741 agtttacggt agaacaacta cgtgagctgg gtattaaggt ggatgaaatc ccggcgctgg
     1801 ctgaaaaaga tgacgatagc gtgatcaact cgcttgaaca aatcattccc ggtacagctg
     1861 ctgaatttga tttcaatcat cagcgactta atttgagcat tccccaaatt gcactgtacc
     1921 gtgatgcaaa aggttacgtc tccccttctc gttgggacga tggtatacca acgctgttta
     1981 ccaactactc gtttacaggt tctgataacc gttaccgcca gggcaatcgt agccaacgac
     2041 agtacctgaa tatgcaaaat ggtgctaatt ttggcccctg gcgattacgt aactattcta
     2101 cgtggacacg caacgatcag gcgtcaagct ggaacactat cagtagttat ttacaacgtg
     2161 atatcaaggc gttgaagtct cagttgcttc tgggagaaag cgccaccagc ggcagtattt
     2221 tttccagcta cacctttact ggcgtgcaac tcgcttccga cgataatatg ttgccaaaca
     2281 gccagcgcgg atttgcccca acggtacgcg gtatcgcaaa cagtagtgca atcgtgacta
     2341 tcaggcaaaa tggttatgtg atctatcaaa gcaacgtgcc agcgggtgcc tttgaaatta
     2401 acgatctcta cccctcttcc aacagcggcg atttagaagt cacgattgaa gaaagtgacg
     2461 gtacgcaacg tcgctttatc cagccttatt cttcattacc catgatgcag cgacctgggc
     2521 atctaaaata tagcgcgacc gctggacgct atcgcgctga tgcaaacagt gatagcaagg
     2581 aacccgaatt tgctgaagcc acggcaatat atggtttgaa taatactttt acgctgtatg
     2641 gcggcctgct cggttctgaa gattattatg cgctggggat cggtatcggc ggcacacttg
     2701 gcgcactggg cgcgttgtcg atggatatca acagagctga cacccaattc gataaccggc
     2761 actcttttca tggctatcaa tggcgtacgc agtacatcaa agatatcccg gaaaccaaca
     2821 ccaatatcgc tgttagctac tatcgctata ccaacgatgg ctattttagt tttgatgaag
     2881 ccaatacccg taattgggac tataacagtc gccaaaaaag tgaaattcaa ttcaacatca
     2941 gccagacaat atttgatggg gtaagtctgt atgcctccgg ttcgcagcaa gactattggg
     3001 gcaataacga taaaaacagg aatatctcag ttggggtttc cggtcagcaa tggggaattg
     3061 gttacagcct gaattatcaa tacagtcgct acacggatca aaataatgac cgcgcactct
     3121 ctttgaatct cagtattccg ttagaacgct ggttaccgcg tagccgggtt tcctatcaga
     3181 tgaccagcca gaaagatcgc ccaacccaac atgaaatgcg tcttgatggc tcactgctgg
     3241 atgatggtcg cctgagctat agcctggaac aaagtctgga tgacgataac aaccataaca
     3301 gtagcctgaa cgccagttac cgttcacctt atggcacctt cagtgccgga tacagctacg
     3361 gtaatgacag cagccaatac aattacggcg ttaccggcgg cgtggttatc catcctcatg
     3421 gcgtgacgct ctcgcaatat ctgggcaacg cttttgcgct tatcgatgct aatggggctt
     3481 ctggcgtgag gatacaaaac tatccgggga ttgctactga tccctttggc tatgcagtgg
     3541 ttccttatct cacgacttac caggaaaacc gtctctcggt agatactacg cagctgcccg
     3601 ataacgtcga tcttgagcaa acaacacagt ttgtggtgcc caacagaggt gcaatggtag
     3661 cggcgcgttt caacgccaat atcggttatc gcgtacttgt tacagtcagc gatcgcaacg
     3721 gtaaaccgtt gccctttggc gctcttgcca gcaacgatga gacggggcaa caaagtatcg
     3781 tcgatgaggg cggcatacta tatctctctg ggatatcgag taaatcacaa agctggactg
     3841 tacgctgggg aaatcaggca gatcaacaat gtcagtttgc ttttagtaca ccggattcag
     3901 aaccaacaac ctctgtatta caaggcacgg cgcagtgcca ttaaggataa aaaaatgaaa
     3961 agagcgcctc ttataacagg acttttgttg atatccacat cctgcgctta tgcctcctca
     4021 ggagggtgtg gagctgacag cactagcggt gcgacaaatt acagcagtgt ggttgatgat
     4081 gttacggtga accagacaga taacgtgaca ggacgggagt ttacctctgc cacgctaagt
     4141 agcactaact ggcaatacgc ctgtacctgc tctgcgggta aggcagttaa acttgtctat
     4201 atggtcagcc ctgtacttac caccactgga catcaagcag gatattacaa actcaatgat
     4261 agtctggata ttaaaaccac attacaggcg aatgacattc caggactcgt gactgaccag
     4321 accgtttctg ttaacacccg attcacacag ataaaaagca acaccgtata ttctgctgca
     4381 acccaaacgg gtgtttgcca gggtgacacg tctcgttatg gacccgttaa tattggtgca
     4441 aataccacct ttaccctgta tgtcaccaag ccatttctcg gctcgatgac cattccgaaa
     4501 acggatattg ccgtcattaa aggcgcatgg gtcgatggaa tgggaagccc gtctacaggt
     4561 gacttccatg atttagtcaa gttatcgatt cagggaaatc tcaccgcccc acagtcgtgc
     4621 aaaattaatc agggcgatgt tattaaggtt aattttggat tcatcaatgg tcagaagttt
     4681 accacccgca atgccatgcc agacggtttt actccagtag actttaatat cacttatgac
     4741 tgtggtgata cttcaaagat taaaaactcg ttgcaaatgc gcatcgacgg tacaactggg
     4801 gtagtagacc agtacaacct ggtcgccaga cgaagaagtt cagacaatgc gcccgatgtc
     4861 ggtattcgta ttgaaaatct cggcggcgga gttgcaaata ttccttttca gaacggtatc
     4921 cttcccgttg atccttccgg gcatggcacc atcaacatgc gcgcctggcc agttaatctg
     4981 gtcggtggtg agctggaaac aggaaaattt cagggcaccg ccaccattac cgtcatcgtg
     5041 cggtaaaaga agacgttgcg ttttgattaa cgcaacgctt cggcactctt acccctgctg
     5101 ctccagcgca tacttataca gcgcattttt cttcacgccg tgaatttctg ccgccagcgc
     5161 cgccgctttt ttcaacggca gttctgcctg tagcagcgcc agcgtgcgca gggcatcggc
     5221 gggtaagtct tcttcctgtg ctttatgacc ttcgataatc agcaccattt cgcctttgcg
     5281 acggttttca tcttctttta cccacgccag cagctcgcca acgggcgcgc cgtgaatggt
     5341 ttcccaggtt ttggtcagct cacgcgccag aaccacgtag cgggattcgc ctaataccgc
     5401 aacgatatct tccaggctat ctaacagacg gtgggtggat tcataaaaaa tcagcgtgcg
     5461 cggctccgct tcaatggctt ttagcgcatc acggcggcct tttgatttgg caggtaaaaa
     5521 gccttcgtaa cagaaacggt cagagggtaa acccgcggcg cttaacgcag tgatagcagc
     5581 acacggcccg ggtagcggca ccacgcaaat ccccgcttca cggcaggtac gcaccagatg
     5641 gtagccagga tcgttaatta gcggcgttcc ggcatcggaa accagcgcaa tgttctgccc
     5701 ttcttgcagc ttcgccagca gcgtttcggc tttttgttgt tcgttatggt cgtgcagcgc
     5761 aaacaaccgg gcattaatcc caaaatgttg cagcaataaa ccggtgtgac gagtatcctc
     5821 ggcggcaatc agatcaacgg cctgtaatac ctctaacgca cgctgggtga tatccgccag
     5881 attgccgatt ggcgtcggta caatgtaaag ctggccctga gaattatccg ccgattggtg
     5941 ttgtttcatt gtgtcgtccg tattgccgat ttaatattga gcattgcgta aaaaaatatc
     6001 actggataca ttatggtacc ctcaacattt tctcgtttga aagccgcgcg ttgtctgcct
     6061 gttgttctgg cagccctgat tttcgccggt tgtggcaccc atactcccga tcagtccact
     6121 gcttatatgc agggcacggc gcaggctgat tctgcctttt atcttcagca gatgcagcaa
     6181 agctctgatg ataccaggat caactggcaa ttactcgcca ttcgtgcact ggtgaaagaa
     6241 ggtaaaaccg ggcaggcggt tgagttgttt aaccaactac cgcaagaact gaacgattct
     6301 cagcgtcgcg agaaaacact gctggcggta gagattaaac tggcgcagaa agattttgct
     6361 ggcgcgcaaa acttgctggc gaaaatcaca cctgccgatt tagaacaaaa ccagcaagcg
     6421 cgttactggc aggcaaaaat cgatgccagc caggggcgtc cttccattga tttactgcgc
     6481 gcgttaattg ctcaggaacc gctgctcggc gcgaaagaaa aacagcagaa tattgatgcc
     6541 acctggcagg cgctctcctc catgactcag gaacaggcga atacgctggt gatcaacgcc
     6601 gacgaaaata ttctgcaagg ctggctggat ctgcagcgcg tctggtttga taaccgtaac
     6661 gatcccgaca tgatgaaagc cgggatcgcc gactggcaaa aacgttatcc gaacaatccg
     6721 ggcgcgaaaa tgctgccaac gcagttggtt aacgtaaaag cgtttaaacc agcctcgacc
     6781 aacaaaatcg ccctgctgtt gccgctgaat ggccaggcag cggtatttgg tcgcactatt
     6841 cagcaaggct ttgaagcggc gaaaaatatc ggcactcagc cagtggcggc tcaggtagct
     6901 gccgcacctg ccgcagacgt agctgaacaa cctcagccgc aaaccgtgga tggcgttgcc
     6961 agcccggcac aagcctcggt tagcgatctg accggtgaac agcctgcggc acagccagcg
     7021 cctgtaagca ccccggcagc aaccaccaca gcggtaagcg cgccagcgaa tccatccgca
     7081 gaactgaaaa tctacgatac ctcatcacaa ccacttagcc agatcttaag ccaggttcag
     7141 caggatggcg cgagtattgt ggttggtccg ttgctgaaaa ataacgttga agagttgctt
     7201 aagagcaaca ccccgctgaa cgtgctggca ctgaaccagc cggagaatat cgaaaaccgc
     7261 gtcaatattt gttacttcgc gctttcaccg gaagacgaag cgcgcgatgc agcgcgtcat
     7321 attcgtgacc agggtaaaca agcgccgctg gtgctgatcc cacgcagttc attgggcgat
     7381 cgcgtagcca atgcgtttgc gcaggagtgg cagaaactgg gcggcggcac cgttctgcaa
     7441 caaaaatttg gttccaccag cgaattacgc gcgggtgtta acggcggttc tggtatcgct
     7501 ttaacgggta ccccgattac tcccagagcg acaaccgact ccggcatgac gaccaacaat
     7561 ccaacgctgc aaaccacgcc aaccgatgac cagttcacca ataatggcgg tcgtgtcgat
     7621 gcggtgtaca ttgtggcaac gccgggtgaa atcgctttta tcaaaccgat gatcgccatg
     7681 cgtaacggta gccagagcgg tgcaatgctg tacgccagct cccgcagcgc acaaggcacc
     7741 gcaggcccgg atttccgtct ggagatggaa ggtttgcagt acagcgaaat cccgatgctg
     7801 gcgggcggta atctgccgtt aatgcagcag gcactcagcg cggtgaacaa cgattattcg
     7861 ctggctcgca tgtatgcgat gggcgttgat gcctggtcgt tggcaaatca tttctcacaa
     7921 atgcgccagg ttcagggttt tgaaatcaac ggtaataccg gaagtctgac ggctaacccg
     7981 gattgcgtga ttaacaggaa gttatcatgg ctacagtacc aacaaggtca ggtagtcccc
     8041 gccagttaac caccaaacag accggcgatg cgtgggaagc acaagcgcgt cgctggctgg
     8101 aaggcaaagg actgcggttt atcgccgcta acgtgaacga gcgtggcggc gagatcgatc
     8161 tgataatgcg tgaaggccgg accaccgttt ttgtcgaggt acgctatcgc cgctctgcgc
     8221 tttatggcgg cgcggcagcc agtgtgaccc gcagcaaaca acacaaatta ttacagactg
     8281 cccgcttgtg gctcgcgcgt cataatggga gttttgatac tgtggattgc cggttcgatg
     8341 tggtagcctt caccgggaat gaggttgagt ggattaagga tgcctttaat gaccactcat
     8401 aattaaggtt taaggattag cgtgcaagaa agaattaaag cttgcttcac tgaaagcatt
     8461 caaactcaaa ttgcggcggc agaggcgctt ccggatgcca tctcccgtgc agccatgacg
     8521 ctggttcagt ctctgctcaa tggcaacaaa atcctctgtt gtggtaatgg aacttccgct
     8581 gccaatgcac agcattttgc tgccagcatg atcaaccgtt tcgaaacgga gcggcccagc
     8641 ttacctgcca ttgcactaaa tactgataat gttgtcttaa cggcgattgc caacgatcgc
     8701 ttacatgatg aagtgtatgc aaaacaggtg cgggcgctgg gtcatgcggg agatgtattg
     8761 ttagccattt ccacccgtgg caacagccgc gatattgtaa aagcggtaga agctgccgtt
     8821 acgcgtgata tgactattgt ggcattgacc ggctatgacg gcggcgaact tgcaggtttg
     8881 ttagggccac aagatgtgga gatccgcatt ccttcgcatc gtagtgctcg cattcaggaa
     8941 atgcatatgc tgacggtaaa ttgcctgtgc gatctgatcg ataacacgct tttccctcac
     9001 caggatgatt aaggagaata catgaaggca ttatcgccaa tcgcagtcct tatttccgcg
     9061 ctgctgttgc aaggttgtgt tgccgctgcc gtagtgggta ccgctgctgt gggtaccaaa
     9121 gccgcaactg acccacgcag tgtcggcacc caggtggacg atggtaccct ggaagtgcgc
     9181 gtgaacagcg cattgtcgaa agacgaacag attaagaaag aaacccgtat taatgtcacg
     9241 gcgtatcagg gcaaagtgct gctggttggg cagtcaccaa acgctgaact ttcggctcgc
     9301 gccaaacaga ttgctatggg cgtagacggt gccaacgaag tgtataacga gattcgtcag
     9361 ggccagccga ttggtctggg cgaagcatct aacgatacgt ggatcaccac caaagtgcgt
     9421 tcgcagctct taaccagcga cctggtgaaa tcgtccaacg tgaaagtgac caccgaaaac
     9481 ggtgaagtgt tcctgatggg gctggtgact gaacgtgaag cgaaagcggc ggcagatatt
     9541 gccagccggg tgagcggcgt aaagcgggta accacagcgt ttacgtttat taaatagcag
     9601 ccccttgtaa tgcctgatgc gacgcttgcc gcgtcttatc aggcatacaa cgatgcaaac
     9661 cgtagatcgg ataaggcgtt tacgccgcat ccgacatgtt ttccctcaaa tcagaacaac
     9721 agcgccagcc cgccgacaat cacaccagac actgccacca tcgcccctgt cagccataag
     9781 gctttcgccg ggaacgcttt gcgcagcata atcagtgacg gcaaactcac tgccgggagc
     9841 gtcatcaaca atgccagcgc cggagcggtt cccatacctg ccagcatcat cgtttgtaca
     9901 atcggaattt ctgctgccgt gggaatgaca aacaagcatc ctgccaccgc catcgccacc
     9961 acccacatca ggctgttatc gacagcacca tcggcatggg ggaataacca gacgcgagcg
    10021 gcacccaaca ccagtactgc aaggatgtaa accgggatcg tactccagaa aagcgtccat
    10081 agcgccctgc cccagcggct aaaaaaaccg ccctgtgctt ccggtatgtc aatttcgacc
    10141 ggtgcctgcg tttgcggtgt ttcacgcacc catttttgca ccagcgtcgc aatcagcaac
    10201 accatcacca gcccggccac cagacgaatc gccgcaaaac cccagccgag gacaaacccc
    10261 ataaacacca gcgtcgccgg gtttaacacc ggattgccca tccagaatgc cagcgcaccg
    10321 cccatcgaca cctgttggcg acgcattccc gccgcgaccg gagccgcaca gcaggtacac
    10381 atcatgcccg gcagcgaaaa cagcgttccc agcagcgtgc cgcgaaagcg cgattgccca
    10441 agcgtacgca acaaccagtc acgcgggatc agcacctgaa tcaacgaacc gaggatcacc
    10501 cccagcactg ccgctttcca taccgcgagg aaatagatca tcgcgtaatc caacgccgcc
    10561 tgccacggat tagcatccgc ctgcgcaagg atagatttac cgatactgtg ggtttcggca
    10621 gcagtaaacg ctttgccgta gtaaggttgc catttcacat accagagacc ggcaatgaca
    10681 acgagaaaga aaagcgcggg tttccaccac tgaatgggcg ttgccgcctg agatgaagac
    10741 tgaccagtca tagcattccc caggaataag ttatgataat tgagcgcgtg aatattacgc
    10801 tcactatcaa ttcttgggaa taattattca gctcttttgc gtaattctga agagcttaaa
    10861 atcgtcacac cttcatgccc gggccgcatc gcctctgcca gcatgacccg cgccacgtct
    10921 ctcgcatcaa tggatttcca gttaccgggt aataaacgga acagcggcgc aaaaagcgtt
    10981 tcgttcatcc gctgtttgct acgatcgccc agtaacatcg acgggcgagc aatggtcaat
    11041 ttcggccagt tctgggcgat taatgcttct tccatctccc ctttgacgcg gttatagaaa
    11101 aacggcgagt gggcattggc acccatagca ctggccacca gcatatgctg cgcgcccagt
    11161 cgccgcccgg ttaatgcggt atccactacc agcgtgtaat cggcatgaat aaacgcctct
    11221 ttgctccccg cttctcgccg cgtggtgccg agacaacaaa acacaatgtc gatgggatcg
    11281 gtgacctgcg ccagcgcgtc agtcagttgc ggatcatggg gattaaacac cccgggcata
    11341 tcgcccaacg gacgtcgcgt cggcgcggct atggcgttaa ctttcggttc gttaatcaac
    11401 atccgcagca ggtgaccgcc caccagcccc gttgcgcctg taatcagtac ctgactcatc
    11461 ttcgctcctt tacagaattg tctgccttgc gctccacggc tcatgcacca ggcttaatag
    11521 accatgaggt aattatcccc gattgtggga aattcgcctc atccaatgca acaacgcgga
    11581 ggaagcatga gtaagaaaat tgccgtttta atcactgatg aatttgaaga ttcagaattt
    11641 acttcacccg cagacgagtt ccgtaaagcc ggacacgaag tgattaccat tgaaaaacaa
    11701 gcgggtaaaa cggtgaaagg caaaaaagga gaagccagcg tgaccatcga taaatccatc
    11761 gatgaagtga cgccagcgga gtttgatgcc ctgctgctac cgggcggcca ttcaccggat
    11821 tatctgcgtg gggacaaccg ttttgtcacc tttacccgtg attttgtgaa tagtggcaaa
    11881 ccggtgtttg ccatctgtca cggcccgcag ttgctgatca gcgccgatgt gattcggggg
    11941 cgcaaactga ccgcagttaa accgatcatt attgatgtta aaaatgcggg cgcggaattt
    12001 tacgatcagg aagtcgtggt tgataaagat cagctggtta ccagccggac accggacgat
    12061 ctgccagcgt ttaaccgcga ggcgttacgc ctgctcggtg cctgagtcgc gtagccaaat
    12121 cattttttta ccaaagccca gcgtgttgtc ggtgaatttg atttcatcga gccggatttc
    12181 ccacaccggg gccgacagca ttctggcaac cggaaagcga cgattgtacg ccttgcgcgc
    12241 gaggtcgctt tcctcacctt ccagcctgcg gatctcacct ttaaactgca caccgcgaat
    12301 taacgctacc gttttcggct gaccgtttac cgttccggca actgccgcct gcggcccgct
    12361 catctgcgcg tggcgcgttt tttcttccgt caaaatgtag aaggcgactt tctgcgcatc
    12421 aaaaagataa aaggcattag cgcaccagag ttccccttcc tgctgcacac accaggtgac
    12481 aacatgttgt tttgccagcc aacggctgat ggcgatgagt gtttccattg ctgttctctt
    12541 ttatactgtg ggcctgacct taacatgcga tgcagacgat gacaccctgg tttctttact
    12601 tgatccgtac cgccgacaat aagctttata ccgggatcac cacggatgtc gaacgccgct
    12661 atcagcagca ccaaagcggc aaaggggcga aagcactgcg cgggaaagga gaactgacgc
    12721 tggcgttttc tgctccagtg ggcgatcgtt cgctggcgtt acgggcggaa tatcgcgtta
    12781 agcaactgac caaacggcaa aaagagcgtc tggtagcgga aggcgcaggg tttgcagagc
    12841 tgttaagcag tctgcaaacc ccggagatta aaagcgattg aaatgctcgt gatactcaac
    12901 caggccggta acgccattca gcgcgtcatc cgctaaacga tgtacctgga aggcgctttc
    12961 agtgcccggc cagcggcaac gcagatcgtg atgcgccgcc agttcaaagc cgaaacgact
    13021 gtacagcgcc ggatcgccca gcgtcaccac tgcggcatag ccgaactcat taagcgaatc
    13081 gagtccttca tagaccagtt ggcgtgccag cccctgtcca cggtattttt catcaaccgc
    13141 caacggtgcc atgccgaccc attgcaggtc ttcgccctgc acatcaaccg gactaaatgc
    13201 cacatagcca atgacctgac cttcgtcatc tgtcgccacc agccccagcg tcagaaagcc
    13261 atcttcacgc agatcgtgaa ccagcttcgc ttccgcatca ctttcgaatg agcgacgcag
    13321 cagggcatca atacccggcg catcaatggg aatttctact cgaattagca tggttcacct
    13381 accgatgtct gtttggtttc aggcgcggtt ttcattcccg cctcaacaaa atccgccagt
    13441 tgcagcagca tcatgcgtaa ggctttcggc atttgctcca gttcaatggc gtccatcagg
    13501 tttttcacat acagccccag ctccgtatcg ccttcaatca ccagccgacg ctggaagaag
    13561 agcgtatccg gatcttgttt acgcgccgca atcatcagca gatcgctggc gtcggcacta
    13621 aaactcacat cagcttgcgc gttctggctg accaccagtt tgccattcac caccgaggta
    13681 aaccattgca ggtcaatatc acgcacatga atacttaacc agcggccttc aagaaactcc
    13741 agctcaccat catccagcgc ctgacggaat tgccagctta agacctgctc aagaacctgg
    13801 cgttttagcg caaatggcgt cagttttacc ggtacactca acagagatgg ccccaaatgc
    13861 acaatacggg aacgcagttt atccaacacg agttttactc cctgtttcaa caatcatcct
    13921 attttgccat atcagaaaaa taacatagcg gtataaatca acaattccat atgaaattgc
    13981 tgctaccacc aatacaactt taactgcctt aaatcaaaaa ttgtcgcagc aaggttaact
    14041 aaaatcccag ttcgttaaca tttttgcgtt ttgatagcgc aaccttcagg aaaaattatg
    14101 gagctgctct gccctgccgg aaatctcccg gcgcttaagg cggccatcga aaacggcgca
    14161 gatgctgttt atatcgggct aaaagatgat accaatgccc gtcacttcgc cggccttaac
    14221 tttaccgaga aaaaattgca ggaagcggtg agttttgtcc atcaacatcg ccgcaaactt
    14281 cacatcgcga ttaacacttt tgcgcatccg gacggttacg cccgttggca gcgcgccgtg
    14341 gatatggcgg cgcagctggg tgccgacgcg ctgatcctcg ccgacctcgc catgctggaa
    14401 tacgccgccg agcgttatcc gcatattgag cgccacgtat cggtgcaggc ttcggcgacc
    14461 aatgaagagg cgattaactt ttatcatcgc cattttgacg ttgcccgcgt ggtgctgccg
    14521 cgcgtgttgt cgattcatca ggtgaaacaa ctggcacggg tcacacctgt accactggaa
    14581 gtctttgctt tcggcagcct gtgcattatg tcggaaggtc gttgctatct gtcgtcgtat
    14641 ctgacgggtg agtcgcccaa caccgtaggc gcgtgttctc cggcccgttt cgtgcgctgg
    14701 caacaaacgc cgcaggggct ggaatcccgc ctgaacgaag tgctgatcga ccgttatcag
    14761 gacggcgaaa acgcaggtta tccgacgcta tgtaaagggc gttatctggt ggacggcgag
    14821 cgctatcacg cgctggaaga accaaccagt ctcaataccc tggaactgct gccggagtta
    14881 atggcggcga atattgcttc ggtgaaaatt gaaggccgcc agcgtagccc ggcgtatgtc
    14941 agccaggtgg cgaaagtctg gcgtcaggct atcgaccgtt gtaaggccga tccgcaaaac
    15001 tttgtaccgc aaagcgcgtg gatggagacg ctcgggtcga tgtccgaagg cacgcaaacc
    15061 actcttggcg cgtatcaccg taaatggcag tgagaagagc aatgaaatat tccttagggc
    15121 cagtgctgtg gtactggcca aaagagacgc tggaagattt ttatcagcag gccgccacca
    15181 gcagcgccga cgtgatttat cttggtgaag cggtatgcag caagcgccgg gcaaccaaag
    15241 ttggcgactg gctggagatg gcaaaatcgc tcgccggaag tggcaagcag attgtgctct
    15301 ccacgctggc gctggtgcag gcatcctctg aactgggcga actgaaacgc tatgttgaga
    15361 acggtgagtt tctgattgaa gccagcgatc tcggcgtggt gaatatgtgc gctgaacgca
    15421 aactgccgtt cgtcgccggg cacgcgctga actgctacaa cgcggtgacg ctgaaaatat
    15481 tgctcaaaca gggcatgatg cgctggtgta tgccggtgga gctttcccgc gactggctgg
    15541 tgaatctgct taatcagtgc gatgagctgg gcattcgcaa ccagtttgaa gtggaagtcc
    15601 tgagctacgg tcatctaccg ctggcctact ccgcccgctg ctttaccgcg cgttcggaag
    15661 accgcccgaa agatgagtgt gaaacctgct gcattaagta tccgaacggg cgcaacgtgc
    15721 tgtcgcagga aaaccaacaa gtgtttgtac tcaatggcat tcagaccatg agcggctacg
    15781 tttacaacct cggtaacgag ctggcatcca tgcaggggct ggttgatgtg gtccgcctgt
    15841 caccgcaggg tactgacact ttcgcgatgc tcgacgcctt ccgcgctaat gaaaatggcg
    15901 ctgcgccact accgttgacg gcaaacagcg actgtaacgg ctactggcgg cggctggcag
    15961 gactggagct gcaagcctaa gtaaatatgc tcgtcatact tcaagttgca tgtgctgcat
    16021 ctgcattcgt tcaccccagt cacttactta tgtaagctcc tggggattca ctctcttgtc
    16081 gccttcctgc aactcgaatt atttagagca cagctcactt tgttaacaac tttaactact
    16141 ctttaatgca gtattaaaga ttaatcggta acaaagtgag ctgttatgac tgataaaacc
    16201 attgcgtttt cgctactcga tctggccccc attcccgaag gttcttcagc gcgggaagca
    16261 ttctcccgct ctctcgacct cgcccgtctg gctgaaaagc gcggctatca tcgctactgg
    16321 ctggcggaac accacaatat gaccggcatt gccagtgctg caacatcggt attgattggc
    16381 tatctggcgg cgaataccac cacgctgcat ctggggtctg gcggcgtgat gttgcctaac
    16441 cactcaccgt tggtcattgc cgaacagttc ggcacgctca atacgctcta tccggggcga
    16501 atcgatttgg ggctgggtcg tgctccgggt agcgatcagc gaaccatgat ggcgctacgt
    16561 cgtcatatga gcggcgatat tgataatttc ccccgcgatg tcgcggagct ggtggactgg
    16621 tttgacgccc gcgatcccaa tccgaatgtg cgcccggtac caggctatgg cgagaaaatc
    16681 cccgtgtggt tgttaggctc cagcctttac agcgcgcaac tggcggcgca gcttggtctg
    16741 ccgttcgcgt ttgcctcaca cttcgcgccg gatatgttgt tccgggcgct gcatctttat
    16801 cgcagcaact tcaaaccgtc ggcacgactg gaaaaaccat acgcgatggt gtgcatcaat
    16861 attatcgccg ccgatagcaa ccgcgacgct gagtttctgt ttacctcaat gcagcaagcc
    16921 tttgtgaagt tgcgccgtgg cgaaaccggg caactgccgc caccgattca aaatatggat
    16981 cagttctggt cgccgtccga acagtatggt gtgcagcagg cgctgagtat gtcgttggta
    17041 ggtgataaag cgaaagtgcg tcatggcttg cagtcgatcc tgcgcgaaac cgacgccgat
    17101 gagattatgg tcaacgggca gattttcgac caccaggcgc ggctgcattc gtttgagctg
    17161 gcgatggatg ttaaggaaga gttgttggga tagtgtgtct taacgcggga agccttatcc
    17221 gagctggcaa cgctgtccta cgtagacctg ataagcgaag cgcatcaggc attgtgtagg
    17281 cagcagaaat gtcggataag gcaccgctga ttactgatac accggcagta aattaaagct
    17341 cgataaaata tggaccagtg cgttgccaac gccaaacacc agaatcagcg taatcatcgg
    17401 cttgccgccc cagacgcgga atttcgggct gccaaagcgt ttacgcgatg cgcgggctaa
    17461 cagcgccgga acaattgccg cccagatggt agctgctaaa ccagcataac caatggcgta
    17521 caggaatccg ttcgggaaca gtagaccacc cacaacaggc gggacaaagg tcagcaatgc
    17581 cgttttcaag cgccccaggg ctgagtcatc gaaaccaaac agatctgcca gatagtcaaa
    17641 caaacccagc gttacgccaa ggaacgaact cgctaccgca aagtttgaga acacgaccag
    17701 cagaaggtcc aggctacggc tgttcagcac gccgcttaat gcctgtacca gcacatcaat
    17761 attaccgccc ttctctgcaa taccgataaa ctccggacgc gggatgttac ccatcgtcgc
    17821 cagcaaccag atggtataca gcgccagtgc catcagcgta ccgtacacca ggcatttcac
    17881 gatggttttc ggatctttgc cgtaatactt catcaggctt ggcacgttac cgtgataacc
    17941 aaacgacgcc aggcagaacg gcaatgtcat cagcagatac ggcgcataag atgcattgct
    18001 ttctgcaacg ttgaacaatg tcgcaggctg cacatgcccc aacaggctgc caaaggtgag
    18061 gaagaaggta atgactttcg cccccagcac aatcgccgtc atgcggctga cggccttagt
    18121 actcaaccac accacaaacg ctaccagcag tgcaaaacca aaacccgccg cccgtgccgg
    18181 gacgtttagc gacatctctg cgaaggtgtg atgcagaatt gaaccactgg cagaaatata
    18241 ggcataggtc aggatataga gcacaaaggc aatggaaata ccgttgacca cgttccagcc
    18301 tttgcccagc aaatctttgg tgatggtgtc aaaactcgaa ccgattcggt aattcaggtt
    18361 agcttccaga atcatcaagc cggaatgcag catacagaac caggtaaaga tcagcgccgc
    18421 cattgaccag aaaaaccacg ccccggacat gaccactggc agagaaaaca tccctgcgcc
    18481 aataatggtg ccgccgataa tcaccacgcc gccaagcagc gacggtgacg tttgggtggt
    18541 ggttagtgtt gccatgaggg cttctctcca gtgaaaaata gtgcgactgc gttgttatgc
    18601 attgcactgt accagtacac gagtacaaaa gacagaaaaa aagccccgat ggtaaaaatc
    18661 ggggctgtat atattatttt acagattgtg ttcgctgttc agcgatgatt acgcatcacc
    18721 accgaaacga cgacgaccgg tagagtcatc acgacgcgga gcgcggcctt cacgacgttc
    18781 gccgctaaaa cgacgaccat caccacggcc accttcacgg cgttcaccac tgaagttacg
    18841 accgccttca cgacgttcgc caccgaaacc acgaccaccg ccacgacgct caccgccagt
    18901 atgcggctgt gcatcgccca gtaactgcat gttcatcggc ttgttgagaa tgcgagtacg
    18961 cgtaaagtgt tgcagcactt cacccggcat acctttcggc agttcgatgg tggagtgaga
    19021 agcaaacagc ttgatgttac caatgtaacg gctgctgatg tcgccttcgt tagcaatcgc
    19081 accaacgata tgacgaactt caacaccatc atcgcggccc acttcaatgc ggtacagctg
    19141 catatcgcca acatcacgac gttcacgacg cggacgatct tcacggtcac cacgcgggcc
    19201 acggtcgtta cgatcgcgcg gaccacggtc atcacggtca cggaattcac gtttcggacg
    19261 catcggcgca tctggcggta cgatcagagt acgttcaccc tgtgccattt tcagcagtgc
    19321 cgcagccaga gtttcgagat ccagctcttc accttctgca gtcggctgaa ttttgctcag
    19381 cagtgcgcgg tattgatcca gatcgctgct ttccagctgc tgctgtactt tagcggcgaa
    19441 tttttccaga cggcgtttgc ccagcagttc tgcgttcggc agttctactt ccggaatagt
    19501 cagcttcata gtacgttcaa tgttgcgcag cagacgacgc tcgcggttct caacgaacag
    19561 cagcgcgcgg ccagcacgac ccgcacgacc ggtacgaccg atacggtgaa cgtaagactc
    19621 agaatccatc gggatatcgt agttaactac caggctgata cgctcaacgt ccaggccacg
    19681 ggctgcaacg tcggtcgcaa tcaggatgtc cagacgacca tctttcaggc gttccagtgt
    19741 ctgttcacga agcgcctggt tcatgtcacc gttcagcgcg gcgctgttgt agccgttacg
    19801 ctcaagagct tcagccactt ccagagtcgc gtttttggta cgaacgaaga taatcgccgc
    19861 atcaaaatct tccgcttcca ggaaacgtac cagtgcttcg tttttgcgca taccccagac
    19921 agtccagtag ctctggctga tgtcaggacg ggtagtcacg ctggactgaa tgcgcacttc
    19981 ctgcggctct ttcataaagc ggcgggtaat gcgacgaatc gcttccggca tggttgcaga
    20041 gaacagagcg gtctgatgac cttccgggat ctgcgccata atggtttcaa cgtcttcgat
    20101 gaagcccatg cgcagcattt cgtcagcttc atccagaacc agaccgctca gtttagagag
    20161 gtccagagtg ccacgtttca ggtggtccag cagacggccc ggagtaccga caacgatctg
    20221 cggcccctga cgcagggcgc gtaattgcac gtcataacgc tggccgccgt acagagcaac
    20281 cacatttacg ccgcgcatgt gtttagagaa atccgtcatt gcttcagcaa cctgtaccgc
    20341 cagttcgcgg gtcggtgcca gcaccagaat ctgtggtgct ttcagctcag gatcaagatt
    20401 ctgcaacaga ggtaaagaga atgctgcagt ttttccgctc cccgtctggg ccatacccag
    20461 aacgtcgcgg ccattcagca gatgtggaat acactctgcc tgaattggag atggtttttc
    20521 gtaacccaga tcgttaaggg cttcaaggat aggagccttc aggcccagat ctgcaaaagt
    20581 ggtttcgaat tcagccatgt agtacgtgtg cctcaaaatt aatggcggcc agtctacata
    20641 actcatcatg aaattgatca gcaattttca ttgaaaagtg tgaaccggct caaagtaggt
    20701 gtattaacga acaacaacgc cctcacccgt taaggtgatg gcaatcaaaa aagattacgg
    20761 gctgatgtgt acgtcagcta ttgctggtcc gattctgcca ggtcatcttg gtcctggccc
    20821 aggagcgata attccaacaa tgcgtatcgg tgctcaacaa agttatgaac gttgttggca
    20881 accgccagtt tgaacagtgc cgtggcgctg tccaaatccc ccagacttag gtagtactta
    20941 cctaaataga agttggtttc actgagatgc tcagcgagcg aggtgttatc cgttgcgtcc
    21001 gccttgagcc tttccattaa cgtttgttcg ctaatgttgc ccaggtagaa ctcgacaatg
    21061 ttccatcccc actgttcctt atccgatttt tcgaagtgct gtttcaacac ttctttagcc
    21121 tgcttctcat cgagcttctg ctcggcgaga taaagccaca gactacggaa aggatcattg
    21181 ggatcgtctt gataaaacgc cagcagatca tcttgcgcta acttgtcacg accgccgtaa
    21241 tataatgcga tcccgcgatt caagtgcgcg tagttgtaag ttggatcaag ctcaagtaca
    21301 gaatcaaacg cttcataggc agcatcaaaa ttgcctgcct gcgttaaata tatgcctaag
    21361 taattgaata cttcaggcat atccggtcgg attgccagcg cttgcgaaaa atcgttacgc
    21421 gctaatgccc tcagaccgag actatcatac aacactccgc gctcatataa aagctgtgcg
    21481 cgttcgtcat cggttaaagc ccgactggca aggatttgtt ccatacgtgc cagaatcact
    21541 tcctgctgta aagtcggttg caatggtacc gcgaggactt cacttttacg ccaggaagta
    21601 ttactgcatc ctgcaagcgt aagtgctgtc gcaacgaaac accagcgcaa aaaaggcttc
    21661 atttcccact cccgaagacc acggttgaat gaacgtcctg ttcccggttg ctaacaaggc
    21721 gtcctgcccg gttaaaagcc ccccgccgca gcggagggca aatggcaacc ttactcgccc
    21781 tgttcagcag ccggagcttc cggtgctgca gcaggttgag actgctcagt cgcttcttta
    21841 atgctcagac ggatacggcc ctggcgatca acttccagaa ctttcaccgg tacttcctga
    21901 cccatctgca ggtaatcggt cactttctca acgcgtttgt cagcgatttg agagatgtgg
    21961 accagacctt ctttaccgcc gccgatggca acaaatgcgc caaagtcaac gatacgggtc
    22021 actttaccag tgtagacgcg gcccacttcg atttctgcag tgatctcttc gatacgacga
    22081 atagcatgtt tcgctttctc gccgtcggtc gctgcgatct tcacagtacc gtcatcttcg
    22141 atttcgatgg tagtgccagt ttcttcggtc agggcacgga taacagagcc gcctttaccg
    22201 ataacgtctt tgatcttatc cgggttgatc ttgatggtat ggatacgcgg tgcgaactca
    22261 gagatatcgc cacgcggcgc gttgatcgcc tgttccatta cgcccaggat atgcagacgc
    22321 gcacctttag cctggttcag cgcaacctgc atgatctctt tggtgatacc ttcaattttg
    22381 atatccatct gcagtgcaga gataccgtcg cgggaacctg caactttgaa gtccatatcg
    22441 cccaggtgat cttcgtcgcc caaaatgtca gacagtacaa cgtagttgtc gccttctttc
    22501 accagaccca ttgcgatacc cgcaacggca gctttgattg gcacacctgc gtccatcagc
    22561 gccagagacg cgccgcacac ggaagccata gaagaagaac cgttggattc ggtgatttca
    22621 gacactacac gtacggtgta cgggaatttg tccatatccg gcatgactgc cagcacgccg
    22681 cgcttcgcca gacgaccgtg accaatttca cgacgcttcg gagaaccgac catgccggtt
    22741 tcgcctacgg agtacggagg gaagttgtag tggaacagga aggtatcggt acgttcgccc
    22801 atcagttcat caagaacctg cgcgtcacga gcggtaccca gcgttgcagt aaccagtgcc
    22861 tgcgtttcac cgcgggtgaa cagcgcagaa ccgtgagtac gcggcagcac gccagtacgc
    22921 acatccagac cacggatcat atctttttca cgaccgtcga tacgcggttc gcctgccagt
    22981 acgcggctac gaacaacgtt tttctcgata gcgtgcagaa tttcacccag ttcgttttcg
    23041 tccagggttt cgtcttcagc aagcagcgtc gcgatggttt cagatttgat gacatcaacc
    23101 tgcgcataac gctcttgttt gtcggtgatg cggtaagcat cgctcaggcg agcttcagcc
    23161 agtgcagcaa cgcgcgcgtt cagcgcttcg tttaccggct ccggctgcca gtcccaacgt
    23221 ggtttaccgg cttctttcac cagttcattg atgttctgaa taacaacctg ctgttgttca
    23281 tgaccgaaca ctactgcgcc cagcatctgg tcttcgctca gcagttcagc ttcagattca
    23341 accatcagaa cagcggcttc agtaccggca acaaccagat ccagtttgct ctctttcagc
    23401 tcgtcctgag tcgggttcag tacgtactgg tcattgatgt aacctacgcg agcagcacca
    23461 atcggaccat tgaacggaat accagacaga gacagtgctg cggaagcacc aatcatcgcg
    23521 acgatatccg ggttaacttg cgggttaaca gaaaccacgg tggcgataac ctgaacttcg
    23581 ttgacgaagc cttccgggaa cagcgggcga atcgggcggt caatcagacg cgcgatcagg
    23641 gtttcgcctt cgcttgggcg gccttcacga cggaagaagc tacccgggat acgaccagca
    23701 gcgtaggtac gctcctgata gttaacggtc agtgggaaga agtcctgacc tggtttggct
    23761 tttttctggc caacaacggt aacgaatacc gcggtgtcat ccatgctaac cataacagcg
    23821 gcagtagcct gacgagccat catgccggtt tccagagtca cggtgtgttg gccgtactgg
    23881 aatttacgaa cgatcggatt aagcaatgta atatcctttc tctttcttag acagtacctt
    23941 acggcactgg tgttaatacc cgatcttctg cgcatcctcg cgactaatga caaccctaac
    24001 ccagctctat gtgggtaaag cctctcatta gccgcgcgaa cctctgcaac ggaagatcat
    24061 tcatagcaac aatacattag tttccagtga attgctgccg tcagcttgaa aaaaggggcc
    24121 actcaggccc ccttttctga aactcgcaag aattagcgac gcagacccag gcgctcgatg
    24181 agctgggtgt aacgtgctac gtctttacgt ttcaggtagt cgagcagttt acgacgctga
    24241 gaaaccatgc gcagcagacc acgacggctg tggtgatctt ttttgtgctc tgcaaagtgg
    24301 ccctgcaggt ggttgatctg tgcagtcagc agtgctacct gaacttcggt agaaccggtg
    24361 tcgtttgcgt cacgaccaaa ctcagaaacg attttagctg ttgcttcagt acttagagac
    24421 attttaaaac tccaaagtat atagaatgaa aggacgccga tctctaattc agcgatccca
    24481 gtgtacgtta cgcaaagtgt taaacaattt acgcgacgtt aagcggcagt attctactcg
    24541 tagcgacctg ttatcgcaag acggttaaca ttacgccggg tattcaacca ccaggcgacg
    24601 aggcgcaacg cggccttcat cgtcaatttc gcccataccg ataaatttgc cgttctcacc
    24661 ttctgtaacg cgaaccagtc cttccagcgg cgcaccggat gtacgaactg ggttaccatt
    24721 tttgaagtaa acagaagacg ttaacggaag attcaccacc gggtagtccg aagctggact
    24781 gtccattggc atcagtaatg gatcaagtaa ctccgcagcc ggaatatcct gctgttcggc
    24841 ttgttcgacc agttcacgca gatgttccag ggtcaccatc cgttcaaccg gatatttact
    24901 taccgccaga cgacgcaggt aaataacatg cgcgccacaa ccgagttttt cacccaggtc
    24961 atcaatgatg gtgcggatat aagtgccttt tgagcagtga atttccagct ccagctcatt
    25021 gccttcgtgg cgaataaaca gcaattcata aacggtaatc gggcgcgctt cacgcggtac
    25081 ttcaatgccc tgacgcgcat attcgtacag ttttttaccc tgatatttca gtgctgaata
    25141 catcgaaggg atctgttcga tatcgccacg gaaagtatcc agcgccgccg ccagttgctc
    25201 tgcgctaaag gttaccggac gttcttcaac gatttgtccg tcggcatcag aagtatcggt
    25261 acgctgtcca agacgcgcaa tgacccgata gcgtttgtcg gagtccagca gatactggga
    25321 aaacttcgtc gcttccccga ggcaaattgg caacatgcca gttgccagcg ggtccagcgc
    25381 accggtatgc ccggcacggt tggcgttata aatccgtttc actttttgca gcgcatcatt
    25441 gctggacata ccctgcgatt tatccagcaa caaaacgccg ttaatgtcgc gaccgcgacg
    25501 acgaggacga ctcattagtc ctccttgctg tcgtccgggt taacacgacg ttcttcgtca
    25561 tgtttgacca cgctggtcac caggtttgac atgcgcatcc cttcaaccag agagttgtcg
    25621 tagaagaagg tcagttccgg cacgatacgc aggcgcatcg ctttccccag caggctgcgg
    25681 atgaaaccag aagcttcttg caacgctttg atgcccgctt taaccgcgtc ttcatctttg
    25741 tcgttgagga acgtcacata tactttggca tacgccaggt cgcgagacat ttcgacaccg
    25801 gaaacggtgg tcatcatgcc caggcgagga tctttaattt cacgctgcag gatgagagcg
    25861 atctcttttt gcatttcctg cgctacgcgc tgcgggcgac caaattcttt cgccataata
    25921 aattctcctg acaaaaaagg ggctgttagc ccctttttaa aattaatttc aggtggaagg
    25981 gctgttcacg ttggcctgat aagacgcgcc agcgtcacat caggcaatcc atgccggatg
    26041 cggcataaac gccttatccc gcatggaacc ctaaaaacct taagcgatgg tacgttggat
    26101 ctcgatgatt tcgaatactt cgatcacatc gccagtgcgg acgtcgttgt agttcttaac
    26161 gccgatacca cattccatac cgttacggac ttcgttaacg tcatctttga agcggcgcag
    26221 ggactccagc tcgccttcgt agataaccac gttgtcacgc agaacgcgga tcgggttgtg
    26281 acgtttaacc acaccttcgg taaccataca gcctgcgatg gcaccaaatt tcggcgattt
    26341 gaacacgtca cgaacttccg ccagaccgat aatctgctgt ttcagttccg gagacagcat
    26401 accgctcatc gccgctttca cttcgtcaat caggttatag atgacggagt agtaacgcag
    26461 atccaggctt tccgcttcaa tcactttacg tgcagaggca tcagcacgta cgttgaagcc
    26521 aaccaggatg gcgttggacg ccgcagccag ggtggcgtcg gtttcggtga taccacctac
    26581 gccagaaccg atgatcttca ctttaacttc gtcagtagac agtttcagca aggagtcgga
    26641 gatcgcttcc acagaaccct gtacgtctgc cttcaggacg atgttcactt cgtgaacttc
    26701 gccttcggtc atgttggcga acatgttctc gagtttagat ttctgctgac gcgccagttt
    26761 aacttcgcgg aatttaccct gacgatacag tgcaacttca cgcgctttct tctcgtcacg
    26821 tacaacggta acttcatcac ccgcagccgg tacgccggac aggccgagga tttccaccgg
    26881 aatggacgga cccgcttcca gcacttcctg acccagttcg ttacgcatcg cacgaacacg
    26941 accgtattcg aagccacaca gaacgatatc gcccttgtgc agagtacctt cacgtaccag
    27001 aacggtagca accggaccac gacctttatc gaggaaggat tcgataaccg caccgctcgc
    27061 cataccttta cgtaccgctt tcagctccag aacttccgcc tgcagcagga tagcgtccag
    27121 cagttcatcg atgccggtac ccgctttcgc agatacgtgt acgaactggc tttcaccgcc
    27181 ccactcttcc ggcaggatgc catactggga gagttcgttc ttaacacgat ccggatcggc
    27241 ttctggttta tcaatcttgt tcactgcaac caccaccggt acctgcgccg ctttcgcgtg
    27301 ctggattgct tcgatggtct gcggcatcac gccgtcgtcg gcagcaacaa ccaggactac
    27361 gatgtccgtt gcctgcgcac cacgtgcacg cattgaagta aacgcggcgt gccccggggt
    27421 gtccaggaag gtgatcatgc cgttttcagt ttcaacgtgg tatgcaccaa tgtgctgggt
    27481 aatgccgccc gcttcgccag aggcaacttt cgttgaacga atgtagtcca gcagagaggt
    27541 tttaccgtgg tcaacgtgac ccatgatggt cacaaccggc gcgcgcggtt cagccgcagc
    27601 acccgtgtca cggtcgctca ttaccgcctc ttccagctcg ttttcacgac gcaggataac
    27661 tttgtggccc atctcttcag caaccagctg tgcggtttcc tgatcgataa cctggttgat
    27721 ggttgccatt gcgcccagtt tcatcatcgc tttgatgacc tgagagcctt taaccgccat
    27781 cttgttcgcc agttcgccaa cggtgatagt ttcgccgatc acaacgtcac ggttaacggc
    27841 ctgagcaggc ttctggaagc cttgctgcag ggaagaacct ttacgttttc cgcctttacc
    27901 gccacgtact gctgcgcgtg cttcttcacg atcagctttt gattcagcgt gtttgttacc
    27961 tttcttcgga cgcgctgctt tcgcgttacg accacggcca cggccgcctt cgacttcacg
    28021 atcgctttcg tcttcggcct ggcgagcatg ttgagaagta gtgacgtgat aatcgctgga
    28081 atcttcagtc ggttccgcgt tatcagtcca tttgttttct tccgccatac gacgtgcttc
    28141 ttcagcaacg cgacgtgctt cttcttcgag tttacgacgc gcttcttctt cagctttacg
    28201 cttgagctct gcagcttcct gctcacggcg ggctttttca gcctgggcgt ttttagtcat
    28261 atcgtcttgt tgattgctca ctttgtcttt ttccgcagct tcacgtttcg cttgttcagc
    28321 agcttcacgc ttagcttgtt ctgcggcctc acgttcagct ttttgttgcg cctcgcgttt
    28381 agccgattct tctgcctcac gacgggcttg ctcttccgct tcacgctgcg cttgctcttc
    28441 cgctgcaagg cgttcagcct cttgcggatc gcgtttcaca aaggtgcgtt tcttgcggac
    28501 ttcgatttgt accgatttgc tttttccacc ggtaccagga atgttaaggg tgctgcgtgt
    28561 tttacgctgc agcgtcaatt tgtccgggcc tgaatttttc tgattcaggt ggtcaatcaa
    28621 agtctgtttc tcttgtgcag acacagagtc gtcagcagac ttccggatac ctgcatcagc
    28681 aaattgctgt accaggcgtt ccacggaggt ctgtcgctct gcggccagcg ttttaatcgt
    28741 tacatctgtc atgctgttcc ttcctgctac agtttattac gcttcgtcac cgaaccagca
    28801 aatattacgg gcagccataa tcagtgctcc ggctttttcg tcggtcaacc cttcgatatc
    28861 agccagatca tcaatgccct gttcggcgag atcttccagc gtacaaacgc cacgggcggc
    28921 cagtttgaat gccaaatcac gatctacccc ttcaaggttc agcagatcgt cagccggttt
    28981 gttatcaccg aggctttctt cctgggcctg tgcaatggtg gccagtgcat ttttagcacg
    29041 ctcgcgcagt gcttcaacgg tcggctcatc aaggccttcg atttccaaca gctctttcat
    29101 cggcacatag gccagttctt ccagcgtcga gaagccttct tctaccagaa cagtcgcgaa
    29161 gtcttcgtcg atgtcgagat atttggtgaa ggtgtcgatc gctgcgtgcg cttccgcctg
    29221 atgcttagcc tgcaggtcgt caacggtcat cacgttgagt tcccaaccgc tcagctgcga
    29281 agccagacgc acgttctgac cgttacggcc aatcgcctgc gccaggttac cggcttcaac
    29341 ggcgatatcc atggtgtgtt tatcttcatc caccacgata gaagcaacgt ctgccggtgc
    29401 cattgcgtta atcacaaact gcgccgggtt atcatcccac aggacgatat cgatacgctc
    29461 gccgcccagt tcagtagaaa ccgcctgaac acgcgcgcca cgcataccta cacaggcacc
    29521 taccggatcg atacgtttat cgttggtttt caccgcgatt ttcgcacgag aacccggatc
    29581 gcgagccgct gctttaattt caatcacttc ttcgccgatt tctggcactt caatacggaa
    29641 cagttcgatc agcatttccg gcttggaacg agtgacgaac agttgcgcgc cacgcgcttc
    29701 cgggcgaacg gaatagagca cgccacgaac gcggtcgcca gggcggaagt tttcacgcgg
    29761 cagcatatct tcgcgcagga tcacggcttc agcattgttg cccagatcca gagagatgtt
    29821 gtcgcggttt acttttttca ccacgccggt gatgatttca ccttcgtgtt cacggaactg
    29881 atcaaccacc atcgcacgtt cggcttcacg cactttctgc acgataacct gttttgccgt
    29941 ctgggtagtg atacggtcaa aggtaacaga ctcaatctga tcttcaacgt aatcgcccag
    30001 gttcaggctt tcatcttcat aacgtgcggc ttcaagggtg atttccttgg tcggctgggt
    30061 gacttcatca acaactaacc agcgacggaa ggtgtcaaaa tcaccgcttt tgcgatcgat
    30121 ctgtacgcgg acgtcgatct cttgttcata ttttttcttt gttgctgtcg ccagcgcgct
    30181 ttccaatgct tcgaaaatct tctcgcgagg tagcgccttt tcattggata cggcttcaac
    30241 tacagccaaa atttctttgt tcatcgcggg cttttcacct catccagact attaaaagtg
    30301 gggaaccagg ttcgccttct ggatattact cagcgcgaac acttcatctt taccttcgac
    30361 ggtaactgtg atcatttcac cgtctaccgc tttgataacg ccctgccatt tacgacggtt
    30421 ttgtaccgcc atacggagaa ccagagtcac ctcttctccg acaaaacggg cgtagtgttc
    30481 agccgtgaac agtgggcgat cgagacccgg tgaggagact tccaggttat aagcaacggt
    30541 gatgggatct tcaacatcca gcacagcact tacctggtgg ctcacatcag cacaatcatc
    30601 aacattgatg ccatcttcac tatcaatata gatgcgcagt gtggatgtgc gaccgcgaat
    30661 aaattcgatg ccaaccagtt caaaacccag ggcctcaact ggcgcagtaa tcatctctgt
    30721 taatttttgc tctaatgtgg acaagcccac ccccaagaca taaaaaaagg gcctaaagcc
    30781 cagttattct gtagtcagat aacaaaaaac cccgataaat cggggcttta tataactgaa
    30841 ccctataacc gcaactgcgg tctggagcac tttccagaag gattttttca aatcccacta
    30901 cgaaggccga agtcttcaca gtatatttga aaaaggactc taagggaaag tggttgcggg
    30961 ggccggattt gaaccgacga ccttcgggtt atgagcccga cgagctacca ggctgctcca
    31021 ccccgcgcct gaaacgtggc aaattctact cgttttgggt aaaaaatgca aatactgctg
    31081 ggatttggtg taccgagacg ggacgtaaaa tctgcaggca ttatagtgat ccacgccaca
    31141 ttttgtcaac gtttattgct aatcatgtga atgaatatcc agttcacttt catttgttga
    31201 atacttttgc cttctcctgc tcccccttaa gcgcattatt ttacaaaaaa cacactaaac
    31261 tcttcctgtc tccgataaaa gatgattaaa tgaaaactca tttattttgc ataaaaattc
    31321 agtgagagcg gaaatccagg ctcatcatca gttaattaag cagggtgtta ttttatgacg
    31381 acgattctca agcatctccc ggtaggtcaa cgtattggta tcgctttttc cggcggtctg
    31441 gacaccagtg ccgcactgct gtggatgcga caaaagggag cggttcctta tgcatatact
    31501 gcaaacctgg gccagccaga cgaagaggat tatgatgcga tccctcgtcg tgccatggaa
    31561 tacggcgcgg agaacgcacg tctgatcgac tgccgcaaac aactggtggc cgaaggtatt
    31621 gccgctattc agtgtggcgc atttcataac accaccggcg gcctgaccta tttcaacacg
    31681 acgccgctgg gccgcgccgt gactggtacc atgctggttg ctgcgatgaa agaagatggc
    31741 gtgaatatct ggggtgacgg tagcacctat aaaggaaacg atatcgaacg tttctatcgt
    31801 tatggtctgc tgaccaatgc tgaactgcag atttacaaac cgtggcttga tactgacttt
    31861 attgatgaac tgggcggccg tcatgagatg tctgaattta tgattgcctg cggtttcgac
    31921 tacaaaatgt ctgtcgaaaa agcctactcc acagactcca acatgcttgg tgcaacgcac
    31981 gaagcgaagg atctggaata cctcaactcc agcgtcaaaa tcgtcaaccc aattatgggc
    32041 gtgaaattct gggatgagag cgtgaagatc ccggcagaag aagtcacagt acgctttgag
    32101 caaggtcatc cggtagcgct gaacggtaaa acctttagcg acgacgtaga aatgatgctg
    32161 gaagctaacc gcatcggcgg tcgtcacggc ctgggcatga gcgaccagat tgaaaaccgt
    32221 atcatcgaag cgaaaagccg tggtatttac gaggctccgg ggatggcact gctacatatc
    32281 gcttatgaac gtctgttgac cggtattcac aacgaagaca ccattgagca gtatcatgca
    32341 catggtcgtc agttgggccg tctgctgtac caggggcgtt ggtttgactc ccaggcgctg
    32401 atgctgcgtg actctctgca acgctgggtt gccagccaga tcactggtga agttaccctg
    32461 gagctgcgcc gtgggaacga ttattcaatc ctgaataccg tctcagagaa cctgacctac
    32521 aagccagagc gtctgacgat ggaaaaaggc gactcggtgt tctcgccaga tgatcgtatt
    32581 ggtcaattga ccatgcgtaa tctggatatc actgataccc gagagaaact ttttggttat
    32641 gccaaaactg gcctgctctc ctcctctgcc acttcaggcg tgccgcaggt ggagaatatg
    32701 gaaaacaaag gccagtaatt cgacatcaac cctgcccttc aggggtgggg ttaccttgct
    32761 tatcctctgt aaactccttt ctgagcatca cataatcgaa aacatcatgg tttgcatcca
    32821 caacccgtga gtctccttta tagtgtgcaa tcacttcctc cagcgtttgc ggctgttccg
    32881 gctttgttac ccccatccac gcattaataa ggtaattatt gtacgcagtg gaaaagatat
    32941 cgttttctgt tgtgcgaacc acgccatccc caagaacggg gctataccag ataaacatcg
    33001 ggacatgata tgcctgctgg ctggcctccc tgcctccatg aaaatagacg ttcttcttcg
    33061 tagggtcgcg ttccaggccg tggtcggcaa aatacatgac cgaggcgcgg cgatctttta
    33121 ataattcaaa aacctgacct agcaaactgt ctgtgtaatg aatggagttg tcatagcagg
    33181 catcctgatc gtcctgcggt tgaaacacgg cgctggattg cggataggcg ctacaagccg
    33241 gttcatggct tccatttaaa tgaagaacaa tcagtttctt ttgctgcgtc ttttgctgta
    33301 acgcttgcga taaatgcggc aacaacaatt catcaaatcc tctgacatag actgtttcca
    33361 tggcgcgcat ggcgatactg gtaactgctg taccattctg ccgaaaagcg gattgcgagc
    33421 ttagccagaa agtctgaaat cctgcttgat tagccatatt aataatgttg tcggggtaat
    33481 tatgaatgtc atgactcaaa acagaatcag cagttaaaga aaggggaacc gacagcgcag
    33541 tgtaaggtgc gccgcttatt gcttgattaa acagtttgat ctgttttctt tgtgcttcaa
    33601 cttgcggtgt cgtagagcgt gtatatccat acaaagacat attgtcgaca cgtacagact
    33661 cccccacaat caacacgtag gtatcaatac ctgtatccct gactgataat tgaaaatacg
    33721 gcaccgtgtt tgcaattgag agtaatcttt gatgctcttt cgctgctaaa gcaaaatagt
    33781 tgagattaaa aaacggcgta taggtagcaa atcgcgacgc tagtatatat ggactgaacg
    33841 cttttttatt ttttgcatct ttataagcaa attgacaagc ggaaaataaa ctgcccgaaa
    33901 tgacaatcag caataatatt cctgtcactt tttttgtcgg caaggaaaca tcatatttta
    33961 ttattactgc caaaaaaaga agggataaaa aagcaaaagc acatagataa gggatataca
    34021 tccccagcat tttgacaact tcatccggat cactctgcag cacactaatc gcaaatccat
    34081 cattgaaagt tgtaccaaag gtacaccatg aatagaggct aatactcatg tctgccgcag
    34141 tcagtaacac aaatggtgcg gcaacaagca ggcgaagcgg aagggatgat atacgtttaa
    34201 caaccaggat ggtcaggacg aagaacagca atcgctgaac catatgcgct ccggaagagg
    34261 cgactaagtt cgtaatacca aaaacaataa cacaaagata caacaaccaa tgagatttaa
    34321 aacttctagc aaatttgttg aatactgtca tccagaaatc caagttcaca a
//
LOCUS       DAPGWD010000035        33630 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPGWD010000035 DAPGWD010000000
VERSION     DAPGWD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.035"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            16..252
                     /locus_tag="RHX88_001638"
     CDS             16..252
                     /locus_tag="RHX88_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDV2230336.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            255..1112
                     /locus_tag="RHX88_001639"
     CDS             255..1112
                     /locus_tag="RHX88_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2230337.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1112..3124
                     /locus_tag="RHX88_001640"
     CDS             1112..3124
                     /locus_tag="RHX88_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV2230338.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3125..3646)
                     /gene="sodC"
                     /locus_tag="RHX88_001641"
     CDS             complement(3125..3646)
                     /gene="sodC"
                     /locus_tag="RHX88_001641"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDV2230339.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3727..4623)
                     /locus_tag="RHX88_001642"
     CDS             complement(3727..4623)
                     /locus_tag="RHX88_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDV2230340.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4672..4911)
                     /locus_tag="RHX88_001643"
     CDS             complement(4672..4911)
                     /locus_tag="RHX88_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDV2230341.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5014..5613
                     /gene="nemR"
                     /locus_tag="RHX88_001644"
     CDS             5014..5613
                     /gene="nemR"
                     /locus_tag="RHX88_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDV2230342.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIDLLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            5650..6747
                     /gene="nemA"
                     /locus_tag="RHX88_001645"
     CDS             5650..6747
                     /gene="nemA"
                     /locus_tag="RHX88_001645"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDV2230343.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6828..7235
                     /gene="gloA"
                     /locus_tag="RHX88_001646"
     CDS             6828..7235
                     /gene="gloA"
                     /locus_tag="RHX88_001646"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDV2230344.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7338..7985
                     /gene="rnt"
                     /locus_tag="RHX88_001647"
     CDS             7338..7985
                     /gene="rnt"
                     /locus_tag="RHX88_001647"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDV2230345.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8078..12694
                     /locus_tag="RHX88_001648"
     CDS             8078..12694
                     /locus_tag="RHX88_001648"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDV2230346.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAITGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(12745..13092)
                     /gene="grxD"
                     /locus_tag="RHX88_001649"
     CDS             complement(12745..13092)
                     /gene="grxD"
                     /locus_tag="RHX88_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDV2230347.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13427..14242
                     /gene="mepH"
                     /locus_tag="RHX88_001650"
     CDS             13427..14242
                     /gene="mepH"
                     /locus_tag="RHX88_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDV2230348.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14370..14951
                     /gene="sodB"
                     /locus_tag="RHX88_001651"
     CDS             14370..14951
                     /gene="sodB"
                     /locus_tag="RHX88_001651"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDV2230349.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15097..16266)
                     /locus_tag="RHX88_001652"
     CDS             complement(15097..16266)
                     /locus_tag="RHX88_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2230350.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16432..16521)
                     /gene="ynhF"
                     /locus_tag="RHX88_001653"
     CDS             complement(16432..16521)
                     /gene="ynhF"
                     /locus_tag="RHX88_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDV2230351.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16820..17845
                     /gene="purR"
                     /locus_tag="RHX88_001654"
     CDS             16820..17845
                     /gene="purR"
                     /locus_tag="RHX88_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDV2230352.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17842..18774)
                     /locus_tag="RHX88_001655"
     CDS             complement(17842..18774)
                     /locus_tag="RHX88_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2230353.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            18887..20098
                     /locus_tag="RHX88_001656"
     CDS             18887..20098
                     /locus_tag="RHX88_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDV2230354.1"
                     /translation="MQPGIRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            20389..21537
                     /gene="cfa"
                     /locus_tag="RHX88_001657"
     CDS             20389..21537
                     /gene="cfa"
                     /locus_tag="RHX88_001657"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDV2230355.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21577..22218)
                     /gene="ribE"
                     /locus_tag="RHX88_001658"
     CDS             complement(21577..22218)
                     /gene="ribE"
                     /locus_tag="RHX88_001658"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDV2230356.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22433..23806
                     /gene="mdtK"
                     /locus_tag="RHX88_001659"
     CDS             22433..23806
                     /gene="mdtK"
                     /locus_tag="RHX88_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDV2230357.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(23847..25103)
                     /locus_tag="RHX88_001660"
     CDS             complement(23847..25103)
                     /locus_tag="RHX88_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV2230358.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25411..25487
                     /locus_tag="RHX88_001661"
     tRNA            25411..25487
                     /locus_tag="RHX88_001661"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25445..25447,aa:Val,seq:gac)
     gene            25492..25568
                     /locus_tag="RHX88_001662"
     tRNA            25492..25568
                     /locus_tag="RHX88_001662"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25526..25528,aa:Val,seq:gac)
     gene            25676..25981
                     /locus_tag="RHX88_001663"
     CDS             25676..25981
                     /locus_tag="RHX88_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDV2230359.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26107..27711
                     /locus_tag="RHX88_001664"
     CDS             26107..27711
                     /locus_tag="RHX88_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDV2230360.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(27723..28535)
                     /locus_tag="RHX88_001665"
     CDS             complement(27723..28535)
                     /locus_tag="RHX88_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230361.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(28539..29324)
                     /locus_tag="RHX88_001666"
     CDS             complement(28539..29324)
                     /locus_tag="RHX88_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDV2230362.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29321..29989)
                     /locus_tag="RHX88_001667"
     CDS             complement(29321..29989)
                     /locus_tag="RHX88_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV2230363.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30053..30691)
                     /locus_tag="RHX88_001668"
     CDS             complement(30053..30691)
                     /locus_tag="RHX88_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDV2230364.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30704..32806)
                     /locus_tag="RHX88_001669"
     CDS             complement(30704..32806)
                     /locus_tag="RHX88_001669"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDV2230365.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(32827..33453)
                     /locus_tag="RHX88_001670"
     CDS             complement(32827..33453)
                     /locus_tag="RHX88_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDV2230366.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8067 a         8881 c         8293 g         8389 t
ORIGIN      
        1 aaaggaatct actttgtgaa gtttatgctc aatgcaacag gattgccctt acaagatctg
       61 gtgttcggtg cgtccgtcta ttttcctccg tttttcaaag ccttcgcgtt tggattcgtc
      121 atctggcttg tcgtacaccg cctgcttcgt ggctggatct acgccggtga catctggcat
      181 cccttgttaa tggatttatc gctgtttgcg atttgcgttt gccttgctct ggcaatactg
      241 attgcgtggt aactatgtca attaaaacaa ttaagtattt ctcaacaatc attgtagcga
      301 tagttgcggt tcttgccgga tggtggctgt ggaattatta catgcaatct ccgtggacac
      361 gcgacgggaa aatacgcgca gaaaaggttt ctatcacccc acaggtgtcg ggacgtattg
      421 ttgagctgaa tataaaagac aaccaactgg ttaacgcggg ggatcttctg ctcacgatcg
      481 ataaaacgcc ctttcagatc gccgagctga acgcacaggc tcagctggcg aaagcgcaat
      541 ctgacctcgc aaaagccaat aacgaagcga atcgtcgtcg tcatctctca caaaatttta
      601 tttctgccga agaactcgat accgctaacc tcaatgttaa agcgatgcag gccagtgtta
      661 atgccgcaca ggccacgctg aaacagacac aatggcaact ggcgcaaacg gaaattcgcg
      721 ccccggtgag tggttgggtg actaacctca ctacccgcat cggcgattac gctgacaccg
      781 ggaaaccgct gtttgccctt gtcgacagcc actcgtttta cgtcattggt tattttgaag
      841 aaaccaagtt gcgccatatc cgcgaaggtg caccagcaca aattaccctc tatagtgata
      901 acaaaacgtt acggggtcac gtttccagta tcggtcgggc gatttatgat caaagcgttg
      961 aaagtgactc aagccttatt ccggatgtga aacctaacgt cccctgggtt cgtctcgccc
     1021 aacgcgttcc cgtccgcttt gcgctggata aagttccagg cgatgtcacg ctggtgtcgg
     1081 gcaccacctg tagcattgcc gtaggtcaat aatgaacgca tcgtcatggt ccttgcgcaa
     1141 tttgccctgg ttcaaggcca cgctggcgca atggcgttat gcgttacgca ataccattgc
     1201 catgtgtctg gcgctgacgg ttgcctatta tttgaatctg gatgaaccct attgggcgat
     1261 gacctcggct gcagtggtta gctttcccac cgttggcggt gtcatcagca aaagcctcgg
     1321 acgcatcgct ggcagtttac tgggagccat tgcggcactg cttcttgccg ggcatacgct
     1381 caatgagccg tggttttttc tattgagcat gtcggcgtgg cttggctttt gtacctgggc
     1441 ctgtgcgcac ttcacgaata acgtcgcgta tgcatttcaa ctggcgggct acacggctgc
     1501 catcatcgcc tttccgatgg ttaatattac tgaggccagc cagctgtggg atatcgctca
     1561 ggcgcgcgtt tgcgaggtga tagtcggtat tttgtgcggc ggaatgatga tgatgatcct
     1621 gcctagcagt tccgatgcta cagccctttt aaccgcattg aaaaacatgc acgcccgatt
     1681 actggaacat gccagtttac tctggcagcc tgaaacaaac gatgccattc gtgcagcaca
     1741 tgagggggtg attgggcaga tactgaccat gaatttgctg cgtatccagg ctttctggag
     1801 ccactatcgt tttcgccagc aaaacgcgcg ccttaatgcg ctactccacc agcaattacg
     1861 tatgaccagt gtcatctcca gcctgcgacg tatgttgctc aactggccct caccgccagg
     1921 tgccacgcga gaaattctcg aacaattgct gacggcgctc gccagttcgc aaacagatgc
     1981 ttacaccgtc gcacgtatta tcgccccgct acgcccgacc aacgtcgccg actaccggca
     2041 cgtcgccttc tggcagcgac tacgttattt ttgccgcctt tatctgcaaa gtagtcagga
     2101 attacatcgt ctgcaaagcg gtgtagatga tcatgccaga ctcgcacgga catccgctct
     2161 ggctcgtcat accgacaacg ccgaagctat gtggagcggg ctgcgtacat tttgtacgtt
     2221 gatgatgatt ggcgcatgga gtattgcatc gcaatgggat gccggtgcca atgcattaac
     2281 gctggcagca attagctgcg tactctactc cgccgtcgca gcaccgttta agtcgttgtc
     2341 actgctgatg cgcacgctgg tgttactttc gctattcagc tttgtggtca aatttggcct
     2401 gatggtccag attagcgatc tgtggcaatt tttactgttt ctctttccac tgctggcgac
     2461 aatgcagctt cttaaattgc agatgcccaa atttgccgca ctgtgggggc aactgattgt
     2521 ttttatgggt tcgtttatcg ctgtcactaa tcccccggtg tatgattttg ctgattttct
     2581 taacgataat ctggcaaaaa tcgttggcgt tgcgctggct tggctagcgt tcgccattct
     2641 gcgtccagga tcggatgctc gtaaaagccg ccgccatatt cgcgcgctgc gccgggattt
     2701 tgtcgatcag ctaagccgcc atccaacact gagtgaaagc gaatttgaat cgctcactta
     2761 tcatcacgtc agtcagttga gtaacagcca ggatgcgctg gctcgccgtt ggttattacg
     2821 ctggggtgta gtgctgctga actgttctca tgttgtctgg caattgcgcg actgggaatc
     2881 gcgttccgat ccgttatcgc gagtacggga taactgtatt tcactgttgc ggggagtgat
     2941 gagtgagcgt ggcgttcagc aaaaatcact ggcggccaca cttgaagaat tacagcggat
     3001 ttgcgacagc cttgcccgtc atcatcaacc tgccgcccgt gagctggcgg caattgtctg
     3061 gcggctgtac tgctcgcttt cgcaacttga gcaagcacca ccgcaaggta cgctggcctc
     3121 gtaattactt aattacacca caggcatagc gttcaccgcc accgcccagc ggtttaggtt
     3181 gatcggacat attatcgccg ccaacgtgga ccatcagcgc tttgtctttg atttcatcca
     3241 gtgatttcag acgaggcgcg atgacggcat cggtagcttt gccgtcatta ttgacgacca
     3301 gtgcaggcag atcgcctaaa tgcccggcac cttctggccc ttcatgttta ccggtatttt
     3361 gtggatcaag atgcccgcct gcggattccg cggcgctggc tttgccatct ttggtggctg
     3421 gctggcagct tcctttggca tgaatatgga agccatgttc accggggggt aatgctttca
     3481 gatcgggcga aaactccaga cctttatcgg tttcagtaat ggtgacgcta ccaattgact
     3541 gccctacccc ttgcgacgtg acgaggttca tctcgacttt ttcactggca gcttgtgcgc
     3601 cggttgcaac aaccagcgcc agaatagcca gactaaaacg tttcatagga cctccgttca
     3661 ttgtgcattc ctgaataagt gtaaaccagt gacacatttt tgaacggcgg ctattcctaa
     3721 aagtgcttac ggtacgtcgt accccagtgc cgctttacgg atacgaaacc attgttgacg
     3781 ggtcattttc agtgtttctg cttcgacagc tgcccgtacg cgctcaattt tacctgaacc
     3841 gataattggc agcggctgcg atggtaaacg taatacccag gcgtaaacca cctgttcaat
     3901 cgagcccgcg tttaactcct ctgccaccac agccagttca tcacgcagct gctggaaata
     3961 atcatcatta aacagacgac caccaccaag gcaggaccac gccatcggac gaacacgcag
     4021 ttgttgtagt tggtcgagcg tgccatccag cagtaacggc tgatgcaccg gggatatttc
     4081 cacctgatta gtggcaaggg taaacggcag acgtgattgc aacagggcaa attgcgcagg
     4141 cgtaaagttc gatacgccaa aatgacgcac tttgccgctc tgatgcagat gtttgaacgc
     4201 gtccgccact tcatcggcat ccattaacgg gtctggtcgg tggattaaca gcagatccag
     4261 atgatcggtc gcgagattaa ttagcgactg ttcggcgctc ttaatgatgt gatcgcggtc
     4321 agtgatgtaa tgaccaatga cgttttcttc acgcgcggtc gtcgcgatac cgcatttact
     4381 gacgatttcc atccgttcac gcaggtgagg tgccagtttc agtgcctcgc caaacgccgc
     4441 ttcgcactga tagccaccat aaatatcagc atggtccacg gtggtcacgc cgagatccag
     4501 atgctcttca ataaaactga ccagctggcg ggcggacata ttccagtcca tcaatcgcca
     4561 gtagcccatc acaaaacggg aaaactccgg gccttgcggc gcaatagtaa tacgctgaac
     4621 cataatcgct tcctcttatc agatatgaga ggagtatacg caagattagg ttcaaaagag
     4681 tgatggttgc tccggttcgt ctgatgacgc tggcttattt gcgcgtaatt tgcgcattaa
     4741 tcgctgccga caaaggcgca gcacctcttg tttttcgcca tcgctcattt tattccagtt
     4801 aaaacgctca tcccgactac gaaaacagcc gcgacaaaac ccgcgttcgt cagactggca
     4861 aattccccgg cacgggctct ggacgggaaa gaactctaat tgctccgcca cttcgccctc
     4921 ctcagataag attattacca ttattgaagc tattaatgtc caaagtagca actttgcttg
     4981 cactagaccg actggtctac tacactccaa cgcatgaaca aacacaccga acatgatact
     5041 cgcgaacatc tcctggcgac gggcgagcaa ctttgcctgc aacgtggatt caccgggatg
     5101 gggctaagcg aattactaaa aaccgctgaa gtgccgaaag ggtccttcta tcactacttt
     5161 cgctctaaag aagcgtttgg cgttgccatg cttgagcgtc attacgccgc atatcaccag
     5221 cgactgactg agttgctgca atccggcgaa ggtaactacc gcgaccgcat actggcttat
     5281 taccagcaaa cactgaacca gttttgccaa catggaacca tcagtggttg cctgacagta
     5341 aaactctctg ccgaagtgtg cgatctgtca gaagatatgc gcagcgcgat ggataaaggt
     5401 gctcgcggcg tgatcgacct gctctctcag gcgctggaaa atggccgtga gaaccattgt
     5461 ttaacctttt gtggcgaacc gctacaacag gcacaagtgc tttacgcact atggctgggt
     5521 gcgaatctgc aggccaaaat ttcgcgcagt ttcgagccac tggaaaacgc gctggcccat
     5581 gtaaaaaaca ttattgcgac gcctgccgtt tagcaggcat tttttatcac cagacgaccg
     5641 ggagccttta tgtcatctga aaaactgtat tccccactga aagtgggcgc gatcacggcg
     5701 gcaaaccgta tttttatggc accgctgacg cgtctgcgca gtattgaacc gggtgacatt
     5761 cctaccccgt tgatggcgga atactatcgc caacgtgcca gtgccggttt gattattagt
     5821 gaagccacgc aaatttctgc ccaggcaaaa ggatatgcag gtgcgcctgg catccatagt
     5881 ccggagcaaa ttgccgcatg gaaaaaaatc accgctggcg ttcatgctga aaatggtcat
     5941 atggccgtgc agctgtggca caccggacgc atttctcacg ccagcctgca acctggcggt
     6001 caggcaccgg tagcgccttc agcacttagc gcgggaacac gtacttctct gcgcgatgaa
     6061 aatggtcagg cgatccgtgt tgaaacatcc atgccgcgtg cgcttgaact ggaagagatt
     6121 ccaggtatcg tcaatgattt ccgtcaggcc attgctaacg cgcgtgaagc cggttttgat
     6181 ctggtagagc tccactctgc tcacggttat ttgctgcatc agttcctttc tccttcttca
     6241 aaccatcgta ccgatcagta cggcggcagc gtggaaaatc gcgcacgtct ggtactggaa
     6301 gtggtcgatg ccgggattga agaatggggt gccgatcgca ttggcattcg catttcgcca
     6361 atcggtactt tccagaacac ggataacggc ccgaatgaag aagccgatgc actgtatctg
     6421 attgaacaac tgggtaaacg cggcattgct tatctgcata tgtcagaacc agattgggcg
     6481 gggggtgaac cgtatactga tgcgttccgc gaaaaagtac gcgcccgttt ccacggtccg
     6541 attatcggcg caggtgcata cacagtagaa aaagctgaaa cgctgatcgg caaagggtta
     6601 attgatgcgg tggcatttgg tcgtgactgg attgcgaacc cggatctggt cgcccgcttg
     6661 cagcgcaaag ctgagcttaa cccacagcgt gccgaaagtt tctacggtgg cggcgcggaa
     6721 ggctataccg attacccgac gttgtaatcc aacattgcga gcggcgtaaa gccgccgcta
     6781 tactaaaaca acattttgaa tctgttagcc attttgagga taaaaagatg cgtcttcttc
     6841 ataccatgct gcgcgttggc gatctgcaac gctccatcga tttttatacc aaagtgctgg
     6901 gcatgaaact gctgcgtacc agcgaaaacc cggaatacaa atactcactg gcgtttgttg
     6961 gctacggccc ggaaaccgaa gaagcggtga ttgaactgac ctacaactgg ggcgtggata
     7021 aatacgaact cggcactgct tatggtcaca tcgcgcttag cgtagataac gccgctgaag
     7081 cgtgcgaaaa aatccgtcaa aacgggggta acgtgacccg tgaagcgggt ccggtaaaag
     7141 gcggtactac ggttatcgcg tttgtggaag atccggacgg ttacaaaatt gagttaatcg
     7201 aagagaaaga cgccggtcgc ggtctgggca actaatctcc tgccgggcgt gaactcatcg
     7261 cgcccgcatc tttactgcat cgacaagtaa tatttgtcat aatgcgcgct gcaatttatc
     7321 cgtattaaga gaatcagatg tccgataacg ctcaacttac cggtctgtgc gaccgttttc
     7381 gtggttttta tcctgttgtg atcgatgttg aaacagccgg atttaacgcc aaaaccgatg
     7441 cgctgcttga gattgccgcc atcaccctga aaatggatga acaaggctgg ctgatgccgg
     7501 acaccacatt acatttccac gtcgaaccat ttgtcggcgc aaatttgcaa ccagaagccc
     7561 tcgccttcaa cggcattgac ccgaacgatc ccgatcgcgg cgcggtcagc gaatacgagg
     7621 cgctgcacga aatttttaaa gttgtacgta aaggtattaa agcgagcggc tgtaaccgcg
     7681 ccattatggt ggcgcacaat gccaattttg atcacagctt tatgatggcc gccgcagaac
     7741 gcgcctcact gaaacgtaac ccgttccacc ctttcgccac ttttgacact gctgcactgg
     7801 ccgggctggc actcggacaa accgtattgt caaaggcttg ccagaccgct ggcatggact
     7861 tcgacagcac ccaggcgcac tccgcgctgt acgacaccga acgcactgct gtgctgtttt
     7921 gtgaaatcgt caaccgctgg aaacgtctgg gaggctggcc gctacctgcc gccgaagagg
     7981 tgtaatcgag tcgatgcctg atgacatgca atgattcagg catctatagt gaggctattc
     8041 cacgcatcct gcatgatatt cacggggaat agcgttaatg gcagataatc cagacccttc
     8101 atcgctcctg ccggacgtgt tttcaccggc gacccgcgac tggtttcttc gcgcctttaa
     8161 acagccgacc gctgtccagt cgcaaacctg gcatgtggcg gcgcgaagcg aacatgcgct
     8221 ggtgattgca ccgaccggct ccgggaaaac gctggcagca tttctctacg ccctcgatcg
     8281 gctcttccgc gaaggcggcg aagatacccg cgaggcgcat aagcgtaaaa cctcacgcat
     8341 cctctatatt tcaccgataa aagctctggg caccgacgtt cagcgcaact tgcagctccc
     8401 gttgaagggt attgccgatg aacggcggcg gcgcggcgaa acggaagtca atcttcgcgt
     8461 agggatccgt actggcgata cgcctgcaca ggaacgcagc aaactcaccc gtaatccgcc
     8521 ggatattctg atcaccacac ccgaatcact ctatctgatg ctgacctccc gcgcgcgcga
     8581 aacgctacgc ggcgtcgaaa cggtaattat tgatgaagtc cacgcggtag cgggcagtaa
     8641 acgtggtgcg catctggcgt taagtctgga gcggctcgat gcgctgctcc acacctcagc
     8701 acagcgaatt ggcctttctg ccactgtgcg ctcagccagc gatgtggcag catttcttgg
     8761 tggcgatcgc ccggttacgg tagtcaaccc gcccgcaatg cgccatccgc agatacgaat
     8821 tgtcgtaccg gtcgccaata tggatgatgt ctcatcggtc gccagcagca ccggcgaaga
     8881 cagccatgcc ggccgggaag gctccatctg gccatatatt gaaacgggta tccttgatga
     8941 agtgttgcgc catcgctcga ccattgtctt tactaattca cgtgggctgg cggaaaaact
     9001 gacggcacga ttaaatgagc tttacgccgc acgcttacag cgttccccgt ctatcgccgt
     9061 tgatgcggcc catttcgagt cgacctccgg cgcaacctct aaccgtgtac aaagtagcga
     9121 cgtttttatt gcccgctcac accacggctc cgtctctaaa gaacaacgag caatcaccga
     9181 gcaggcgctg aaatcgggtg agttacgctg cgtggtcgca acctccagtc tcgaactggg
     9241 gattgatatg ggcgcagtgg atctggtgat tcaggtggca acgccgcttt ctgttgccag
     9301 tgggttacaa cgcattggtc gcgccggaca tcaggttggc ggtgtatcta aagggctgtt
     9361 tttccctcgt acccggcgtg acttagtcga ttccgcagtc attgtagagt gtatgttcgc
     9421 aggcaggctg gaaaacctga caccgccgca taatccactc gacgtccttg cgcagcaaac
     9481 cgttgccgcc gcggcgatgg aggcattaca ggtagacgaa tggtactccc gcgtacgccg
     9541 tgccgcaccg tggaaagatc tgccaagacg tgtttttgac gccacgctgg atatgctttc
     9601 cgggcgctat ccctctggcg atttttctgc ttttcgcccc aaactggtct ggaacaggga
     9661 gaccgggata ttgaccgccc gacctggcgc tcaattgttg gcggttacca gcggcggcac
     9721 cattccggat cgtggcatgt atagcgtgtt attacccgaa ggtgaagaaa aggccggttc
     9781 gcggcgggtg ggtgaactgg atgaggagat ggtatatgag tcgcgggtga acgacattat
     9841 cactctcggc gctacctcat ggcggatcca gcaaatcacc cgcgatcagg tgattgtgac
     9901 tcctgctccg ggtcgttctg cccggctccc cttctggcgt ggtgaaggta acggacgtcc
     9961 ggctgaatta ggcgagatga tcggcgattt tcttcatttg ctggcggatg gcgcgttctt
    10021 ttccgggact attcccccgt ggctggcaga agaaaatacg aacgccaata ttcaggggtt
    10081 gattgacgag caacgcaacg cgacgggcat cgttccgggg agtcgccatc tggtcctcga
    10141 acggtgccgt gatgaaattg gcgactggcg tattattttg cactctccct atggaagacg
    10201 ggtgcatgaa ccctgggcgc tggcgattac cgggcgaata catgccctat ggggcgctga
    10261 cgcgtctgtg gtcgccagtg atgacggcat tgttgcgcgc attcctgaca ctgatggtaa
    10321 attgcccgac gccgcgattt ttttgtttga accagaaaag ttgctgcaaa ttgtccgcga
    10381 ggcggtaggc agctcggcac ttttcgccgc ccgttttcgc gaatgcgccg cgcgggcatt
    10441 attaatgccg gggcgcactc cgggccatcg caccccgctt tggcaacaac ggctgcgcgc
    10501 cagtcagttg ctggaaatcg ctcagggata tccggatttt ccggtcattc tcgaaaccct
    10561 gcgcgaatgt ctgcaagatg tttatgatct tcccgcactg gaacgtttga tgcgtcgcct
    10621 gaacggtggc gaaattcaaa tatccgatgt aacgaccact acgccctcgc ctttcgccac
    10681 aagtttattg ttcggctatg tcgcggaatt tatgtaccag agcgacgccc cgctggcaga
    10741 gcgccgggca tccgtactgt cgctggacag cgagttactg cgcaatctac tcggacaggt
    10801 cgatccgggg gaattactcg acccgcaggt cattcgccag gtggaagaag agttgcaacg
    10861 actggctcct ggcagaagag cgaaaggtga agaaggattg ttcgacctgc tgcgcgaact
    10921 ggggccaatg accgttgaag acctggcgca acggcataca ggcagcagtg aagaggttgc
    10981 gtcgtatctg gaaaatcttc ttgcagtaaa acgaatcttc ccagcgatga ttagcggaca
    11041 ggagcgtctt gcctgtatgg atgatgccgc caggctgcgt gatgccctcg gcgtacgact
    11101 accagagtca ttgccagaga tttatttaca tagagtcagt tacccgcttc gcgacctctt
    11161 tctgcgctat ctccgggctc atgctctggt cacggctgaa caactggctc atgagtttag
    11221 tctcggtatt gccattgtcg aagagcagct tcagcaactg cgtgaacagg gtctggtgat
    11281 gaatctgcaa caagacatct gggtgagcga tgaagtattt cgtcgtctgc gtttgcgctc
    11341 gctgcaagcc gccagagaag cgacgcgtcc cgttgcagcc acgacctatg cgcgattgct
    11401 gctggaacgt cagggcgtat tacccgccac cgatggtagc ccggcgctct ttgcctcaac
    11461 atcgccaggc gtttatgagg gcgtagatgg cgtgatgcgg gtgatcgaac agcttgccgg
    11521 agtcggttta cccgcctcac tctgggaaag ccagatcctg cctgcccgcg tacgcgacta
    11581 ttcgtcagaa atgctcgatg aattactggc aaccggtgca gttatctggt cggggcaaaa
    11641 aaaactgggt gaagatgacg gcctggtggc actgcatcta caggaatatg ctgcagaatc
    11701 gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc
    11761 tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga tacgcgacaa
    11821 aatcggcgaa tcggttgatc tctctgccct gcaagaggcg ttatgggcgc tggtctggca
    11881 aggcgtcatc accagcgaca tttgggcacc gttacgcgcc ctcacccgca gcagttccaa
    11941 cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca
    12001 acccgtctcg ccgctggtat cttacaacac accaaatctg gctggacgct ggtcgttatt
    12061 gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa atatgctcga
    12121 ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg gcgggtttcc
    12181 atcgatgcaa acgctttgtc gaagtatgga agactccggg cgaattatgc gaggtcgttt
    12241 tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc gattgcgcga
    12301 tctggcgaca caagccacgc aaacgcgcca ctatacacca gtggcgctct ctgccaacga
    12361 tccggctaat gtgtggggaa atcttctgcc ctggcctgca catccggcaa cgctggttcc
    12421 aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac tctatctggc
    12481 gcaaggtggc aaaaaaatgc tggtctggca ggaaaaagag gaattactcg ccccagaggt
    12541 tttccacgcg ctgactaccg cactgcgtcg cgaaccacgg ctgcgcttta cgctaacaga
    12601 agtgaatgat ctaccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg aggcgggatt
    12661 ttcaagttcg ccacaagggc tggattgggg atagagaaag gactgacgga tgcccgttcg
    12721 catccgtcag tattgcagga cggattattc cgcgtccggc tcttcagact tgtatttagc
    12781 ggcagtttct ttgatcagct gctgcagttc gccacgctga tacatttcga tcacgatatc
    12841 acaaccgccg accagctcgc cgtcaaccca cagttgcggg aaggtcggcc agttagcata
    12901 tttcggcagt tccgcacgaa tgtccggatt ctgcagaata tcaacatagg caaaacgttc
    12961 gccacatgcg gcaagcgcct ggactgcctg ggcagagaaa ccgcagctcg gcagtttcgg
    13021 tgaacctttc atgtacagca ggatcgggtt ttcagcaatc tggcgttgga ttttttcgat
    13081 agtggtgctc attgtcttgc ttcctcaaac ttctttacgg cagtaatctg acattgtagc
    13141 gggtcagtgc ggcatcggaa aataacattt tcatcacgct tttgctattt tatccctttg
    13201 ctctatttgt tgcatttcaa atattcgttt cttctctgat gcgcggcaaa ctggcgtatt
    13261 tataaccatt tttagctttc actgctgcat ttttttgcgc tcgccaacga aacgtatttt
    13321 ttaacaataa aagctattaa ctttctcttc ttctatgcat tagaatcatc aagttttgta
    13381 aatcagacgc aggcatgata gacctgcctt aacagaggga cgctcagtgg cgcggataaa
    13441 ccgtatttcg atcacgctct gtgctttact ttttaccacc ctgcctttaa cgcctatggc
    13501 ccatgcttca aagcaagcca gggagagttc tgctaccact catatcacca aaaaagcaga
    13561 taaaaagaaa agcacggcaa ccaccaaaaa gacccagaaa acagcgaaaa aagccgccag
    13621 taaaagtacg accaaaagca aaaccgcttc ttccgttaaa aaatcttcca ttaccgcttc
    13681 taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa acggcctcag ccagctttac
    13741 cgaaaagtgt accaaacgta agggctataa atcgcattgt gtgaaagtca aaaatgccgc
    13801 gtcaggaact cttgccgacg cgcacaaagc gaaggtgcaa aaagctacga aagtggcaat
    13861 gaataaactg atgcagcaaa ttggtaagcc atatcgttgg ggtggcagct caccgcgtac
    13921 cggttttgat tgcagcggcc tggtttatta cgcttataaa gatttggtga aaattcgtat
    13981 tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca gcgccaatcg aacgtagtga
    14041 actgaaaaac ggcgacctgg tctttttccg tactcaggga cgcggcacag ccgatcatgt
    14101 cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg cgcacaggtc aggaaattca
    14161 aatcacttct ctcagtgaag actactggca gcgccactat gttggcgctc gtcgggtaat
    14221 gaccccaaaa acacttcgct aaaactttac cctgttgtta cggcaacagg gtaagttcat
    14281 cttttgtctc accttttaat ttgctaccct atccatacgc acaataaggc tattgtacgt
    14341 atgcaaatta ataataaagg agagtagcaa tgtcattcga attacctgca ctaccatatg
    14401 ctaaagatgc tctggcaccg cacatttctg cggaaactat cgagtatcac tacggcaagc
    14461 atcatcagac ttatgtcact aacctgaaca acctgattaa aggtaccgcg tttgaaggta
    14521 aatcactgga agagattatt cgcagctctg aaggtggcgt attcaacaac gcagctcagg
    14581 tctggaacca tactttctac tggaactgcc tggcaccgaa cgccggtggc gaaccgactg
    14641 gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt tgccgatttc aaagcgcagt
    14701 ttactgatgc agcgatcaaa aactttggtt ctggctggac ctggctggtg aaaaacagcg
    14761 atggcaaact ggctatcgtt tcaacctcta acgcgggtac tccgctgacc accgatgcga
    14821 ctccgctgct gaccgttgat gtctgggaac acgcttatta catcgactat cgcaatgcac
    14881 gtcctggcta tctggagcac ttctgggcgc tggtgaactg ggaattcgta gcgaaaaatc
    14941 tcgctgcata ataactgatg gcaaatgcag cattgcctga tgcgcttcgc ttaccatgcc
    15001 aacgcggatg aggcgttaac agcgcattcg gcaagataaa tcgcacgttg tcagcaactg
    15061 taacgcagaa ggttatcctt ctgcgttttt gtttaattag ctgttagcaa cgcaaactgt
    15121 ttcaggttgt tttctggctg acataaacac cagcaataat gccagtcccg cgacaatcgc
    15181 tcccatcacc ggcacaaagc tgtatcccag cccagcggaa attaccgcac caccagcagc
    15241 tgctcccagc gcatttccaa gattaaaggc accaatattg actgatgaag acagacctgg
    15301 cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc ggtacgaccg caaaggttgc
    15361 tgcgccccac accaccatgc taatagctgc gccgaactca ttgcgggcca ggaacgggat
    15421 tgccagcata atcaccatca acaacaacaa aaagcctttc aacgtgccgt taactgaacg
    15481 atctgccagt ttgccgccga gatagttacc gatagagaat ccgacaccaa tcagcaccag
    15541 cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt tgcagtaccg gagagatata
    15601 ggtgtagaga gtaaacattg caccagctcc cagtaccgtc gtcagcaatg cagacagcac
    15661 ctgcggacgc attaataccg ccagctcttt tttcacttca ggtcgtgccc ctgcaccacc
    15721 tttaggtaat gagaagaaca gacttaccat tgaaatcact cccagccccg ccgttgccag
    15781 aaatgacatc cgccagccga tggtttcacc caaccaggtc gccgccggca cgccaccgat
    15841 atttgccagg gttaacccca taaacatagt ggcaactgcg ctggcctgtt tatgttttgg
    15901 caccacgctt gcggccacga ctgaacccaa accaaaaaat gctccgtgat tcaggctggt
    15961 caaaatgcgt gaaagcatca gggtcatata atccggcgcg atggcggaaa gtacgttgcc
    16021 gagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg cgggcacgat gagaaagtag
    16081 aagcgtcatc agcggcgcgc ccaccattac gccaactgca taggcactga ttaacattcc
    16141 ggcagcggga atcgagacat ccacaccgcg cgcaatgacg ggcaacaagc ccattggcga
    16201 gaactccgtt gtcccgatac caaacgcgcc aatcgccagc gccagcaacg gatagttaat
    16261 tttcatgcct tatctccacc tcttcgcgtc attacgcgat attcattaaa gtggcgaaag
    16321 catgacagca atcacaaaaa aatgaaaata acaaaaagag aaaacacttt tgccattttg
    16381 ctaacaaaca ggaaggagat gcgagggaga acgcgctccc tcgagaggaa atcagtgcag
    16441 cgcggcagtc aaacccacgg ctacgatcaa accgaggacg ataatcgttg ttaccagtga
    16501 aaatttaagg tcggtgctca tcaagttttc tcctttttta ttaccacaca aaaagtgata
    16561 ttacgcattt ttacacactg tgatgaaaaa atctcccgtc atttataatg ataagtgttt
    16621 ttaccacttc cccttttcgt caagatcggc caaaattcca cgcttacact atttgcgtac
    16681 tggccattga ccccttcctg acgctccgtg tcgtttttcc ggcgtaccgc aacacttttg
    16741 ttgtgcgtaa ggtgtgtaaa ggcaaacgtt taccttgcga ttttgcagga gctgaagtta
    16801 gggtctggag tgaaatggaa tggcaacaat aaaagatgta gcgaaacgag caaacgtttc
    16861 cactacaact gtgtcacacg taatcaacaa aacacgtttc gtcgctgaag aaacgcgcaa
    16921 cgccgtgtgg gcagcgatta aagaattaca ctactcccct agcgcggtgg cgcgtagcct
    16981 gaaggttaac cacaccaagt ctatcggttt gctggcgacc agcagcgaag cggcctattt
    17041 tgccgagatc attgaagcgg ttgaaaaaaa ttgcttccag aaaggttaca ccctgattct
    17101 gggtaatgcg tggaacaatc ttgagaaaca gcgggcttat ctgtcgatga tggcgcaaaa
    17161 acgcgtcgat ggtctgctgg tgatgtgttc tgagtaccca gagccgttgc tggcgatgct
    17221 ggaagagtat cgccatatcc caatggtggt gatggactgg ggtgaagcaa aagctgactt
    17281 caccgatgcg gtcattgata acgcgttcga aggcggctac atggccgggc gttatctgat
    17341 tgaacgcggt caccgcgaaa tcggcgtcat ccccggcccg ctggaacgta acaccggcgc
    17401 aggccgcctt gccggtttta tgaaggcgat ggaagaagcg atgatcaagg tgccggaaag
    17461 ctggattgtt cagggtgact ttgaacccga atctggttat cgcgccatgc agcaaatact
    17521 gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc gatatcatgg caatgggcgc
    17581 actttgtgct gctgatgaaa tgggtctgcg cgtcccgcag gatgtttcgc tgatcggtta
    17641 tgataacgtg cgcaacgccc gctattttac gccggcgctg accacgatcc accagccaaa
    17701 agattcgctg ggtgaaacag cgttcaacat gctgttggat cgtatcgtca acaaacgtga
    17761 agaaccgcag tccattgaag tgcatccgcg cttgattgaa cgccgctccg tggctgacgg
    17821 cccgttccgc gactatcgtc gttaatcacc cgttgcggga gtctcttccg gctcccgcag
    17881 ccactcctta ttcagcgtct cactatcgcc gagatactca agcagccagg ttaacgcagg
    17941 cgacatatca ttttgctgcc atgtcagaca acatgccgaa tccgggaagg ggttttccaa
    18001 ttcaagtgct acccacttcc cctcattaag ccacggtttg gcgaaatgtg ttggcaccat
    18061 ccctatgcat aatcctgccg agatacaggt tgccgatgat tcccagtcag gcacgacaac
    18121 tcttttttgg ttatccagca accaggtaat acgtttaggt agcgttcgcg aggtgtcttc
    18181 gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg aatgggccat ccatcaacgc
    18241 cagcgggtgg tggctggcaa caacgcaact ccagcttagc atccccatat cccggaaggc
    18301 ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatc gccagttcca cgcgcccgtc
    18361 ggaaagcgca tcccagacac cgttgaacac ttcctgaaag acaagaagtt cgacatcatc
    18421 aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt tctggcctga caatattatc
    18481 cactgcgata gctaactgac cgcgccagcc gttcgctatc tgctgacatt gctggcgggt
    18541 gatctgcatt tttttgacaa cagagcgccc ttctttgaga aaccacgctc cagcagcggt
    18601 cagctccaca tcacggtgcc gtcgttcaaa gagcggcacc gccagccact cttccagctg
    18661 acgcacggta tagctgaccg cagaaggaac gcgatgcagc tcctgtgccg cagcgctaaa
    18721 actaccatta cgcgctaccg catcaacaac ttcgagtgaa tattctgacc acatagtctg
    18781 cctgcaaaat ttttgaaacc agtcatcaaa tattaccgtt tcacaacact aatttcactc
    18841 cctacacttt gcggcggtgt ttaattgaga gatttagaga atatacatgc aacctgggat
    18901 aagattttta gtctggctgg cgggtttgag cgtactcggt tttctggcaa ccgatatgta
    18961 tctgcctgct ttcgccgcca tacaggccga cctgcaaacg cctgcgtctg ctgtcagtgc
    19021 cagccttagt ctgttccttg ccggttttgc tgcagcccag cttctgtggg ggccactctc
    19081 cgaccgttat ggtcgtaaac cggtgttatt aatcggcctg acaatttttg cgttaggtag
    19141 tctggggatg ctgtgggtag aaaacgccgc tacgctgctg gtattgcgtt ttgtacaggc
    19201 tgtgggtgtc tgcgccgcgg cggttatctg gcaagcgtta gtgacggatt attatccttc
    19261 acagaaagtt aaccgaattt ttgcgaccat catgccgctg gtgggtctat ctccggccct
    19321 ggctcctctg ttaggaagct ggctgctggt ccatttttcc tggcaggcga ttttcgccac
    19381 cctgtttgcc attaccgtgg tgctgattct gcctattttc tggctcaaac ccacgacgaa
    19441 ggcccgtaac aatagtcagg atggtctgac ctttaccgac ctgctacgtt ctaaaaccta
    19501 tcgcggcaac gtgctgatat acgcggcctg ttcagccagt ttttttgcat ggctgaccgg
    19561 ttcaccgttc atccttagtg aaatgggcta cagcccggca gttattggtt taagttatgt
    19621 cccgcaaact atcgcgtttc tgattggtgg ttatggctgt cgcgccgcgc tgcagaaatg
    19681 gcaaggcaag cagttattac cgtggttgct ggtgctgttt gcagtcagcg tcattgcgac
    19741 ctgggctgca ggcttcatta gccatgtgtc gctggtcgaa atcctgatcc cattctgtgt
    19801 gatggcgatt gccaatggcg cgatctaccc gattgttgtc gcccaggcgc tgcgtccctt
    19861 cccacacgca actggtcgcg ccgcagcgtt gcagaacact ctacaactgg gtctgtgctt
    19921 cctcgcaagt ctggtagttt cctggctgat cagtatcagc acgccattgc tcaccaccac
    19981 cagcgtgatg ttatcaacag tagtgctggt cgctctgggt tacatgatgc aacgttgtga
    20041 agaagttggc tgccagaatc atggcaatgc cgaagtcgct catagcgaat cacactgatc
    20101 tatatcgata tacttatact taggctgcta acaaaatttt gttgtatgat tgaaattagc
    20161 ggcctatact aatttcgagt tgttaaagct acgataaata ttatgttttt acggggacag
    20221 gatcgttccc gactcactat ggatggtcat ttcggcaaag gttcctcctt tccctctgtt
    20281 ctacgtcgga ttatagactc gcggtttttt ctgcgagatt tctcacaaag cccaaaaagc
    20341 gtctacgctg ttttaaggtt ctgatcaccg accagtgatg gagaaactat gagttcatcg
    20401 tgtatagaag aagtcagtgt accggatgac aactggtacc gtatcgccaa cgaattactt
    20461 agccgtgccg gtatagccat taacggttct gccccggcgg atattcgtgt gaaaaacccc
    20521 gattttttta aacgcgttct gcaagaaggc tctttggggt taggcgaaag ttatatggat
    20581 ggctggtggg aatgtgaccg actggatatg ttttttagca aagtcttacg cgcaggtctc
    20641 gagaaccaac tcccccatca tttcaaagac acgctgcgta ttgccagcgc tcgtctcttc
    20701 aatctgcaga gtaaaaaacg tgcctggata gtcggcaagg agcattacga tttgggtaat
    20761 gacttgttca gccgcatgct tgatcccttc atgcaatatt cctgcgctta ctggaaagat
    20821 gccgataatc tggaatctgc ccagcaggcg aagctcaaaa tgatttgtga aaaattgcag
    20881 ttaaaaccag ggatgcgcgt actggatatt ggctgcggct ggggcggact ggcgcactac
    20941 atggcatcta actatgacgt aagcgtggtc ggcgtcacca tttctgccga acagcaaaaa
    21001 atggctcagg aacgctgtga aggcctggat gtcaccattt tgctgcaaga ttatcgtgac
    21061 ctgaacgacc agtttgatcg tattgtttct gtggggatgt tcgagcacgt cggaccgaaa
    21121 aattacgata cctattttgc ggtggtggat cgtaatttga aaccggaagg tatattcctg
    21181 ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg atccctggat taataaatat
    21241 atttttccga acggttgcct gccctctgta cgccagattg ctcaatccag cgaaccccac
    21301 ttcgtgatgg aagactggca taacttcggt gctgattacg atactacgtt gatggcgtgg
    21361 tatgaacgat tcctcgccgc atggccagaa attgcggata actacagtga acgctttaaa
    21421 cgaatgttta cctattatct gaatgcctgt gcaggtgctt tccgcgcccg tgatattcag
    21481 ctctggcagg tcgtgttctc acgcggtgtt gaaaacggcc ttcgagtcgc tcgctaaagg
    21541 ctattctatc gccccctctc cgggggcgat ttcagatcag gcttctgtac ctggttgatt
    21601 catggcattt tctcgtgccg ccagcacacg ttctaccgta tctaccactg cctgagtttg
    21661 tggatcaatt tcaatgttga cgcgtgcgcc gagttttttc ttcccaagag tcgtgcgttc
    21721 cagagtttcc ggaattaaat ggacgcaaaa acgcgttggc gtgacttcgc cgacggtcag
    21781 gctaataccg tcgatgccaa taaatccttt gtacaggata tatttcatca actgactatc
    21841 ctggacttta aaccagatct ggcgattatt ttccgaggtt aatattttcg ccacttcagc
    21901 ggtggtcata atatgacctg acattaagtg tccgccaatt tcatcactga atttcgccgc
    21961 acgctcaacg tttacccaat cccccacttt taaatcgcca agattggtaa tgcgtaacgt
    22021 ttctttcatc aggtcaaaac tgacatggtt gccgttaatt tccgtcacgg tcaggcagca
    22081 accgttatgc gccacggaag cacctgtttc caggccgtcc agcatgtggt cgggtaactc
    22141 caccacatgc gtacgaaaat ttggtttctc gtcaatcgac accagttttg cggtgccctg
    22201 tacaatcccc gtaaacatac ttacaactcc tgaaatcagt taagacattc tgttcagcac
    22261 aatagcaggt ggcaaacgcc cttaccagtg aaggggtaag aatgactatt ttttcactgg
    22321 agaattaata aatcctcgct acaatagact gaatttcccc tgcttcttct ttttgctgcc
    22381 cattcaggcg gctttttagt ctctcatata actacaaata aaaggtgttc acgtgcagaa
    22441 gtatatcagt gaagcgcgtc tgttattagc attagcaatc ccggtgattc tcgcgcaaat
    22501 cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg ggcggctata gtgccaccga
    22561 catggcggcg gtcgctatcg gtacttctat ctggcttccg gcgatcctct ttggtcacgg
    22621 actgctgctg gcattaacgc cggttatcgc gcaattaaat ggttccggtc gacgtgagcg
    22681 cattgcgcat caggtgcgac aaggtttctg gctggcaggt tttgtttccg ttctcattat
    22741 gcttgtgctg tggaatgcag gttacattat ccgctccatg gaaaacatcg atccggctct
    22801 ggcggacaaa gccgtgggtt atctgcgtgc gttgttgtgg ggcgcgccgg gctatctgtt
    22861 cttccaggtt gcccgtaacc aatgtgaagg tctggcaaaa accaagccgg gtatggtaat
    22921 gggctttatc ggcctgctgg tgaacattcc ggtgaactat atctttattt atggtcattt
    22981 cggtatgcct gagctcggtg gcgttggttg tggcgtggct actgcggcgg tgtattgggt
    23041 catgttcctt gccatggttt cttacattaa acgcgcccgc tccatgcgcg atattcgtaa
    23101 cgaaaaaggc accgcgaaac ccgatcctgc ggttatgaaa cgactgattc aactcggttt
    23161 gccgattgcg ctggcactat tctttgaagt gacactgttt gccgtcgtgg ctctgttagt
    23221 gtctccgctc ggtattgttg atgtcgcagg acaccagatt gccctgaact ttagttcact
    23281 gatgtttgtg cttccaatgt cgctggcggc agcggtaact atccgcgtag gttatcgtct
    23341 gggtcagggc tcaacgctgg atgcgcaaac cgctgcgcgg accgggctta tggtgggtgt
    23401 ctgtatggca accctgacgg ccatattcac ggtttcactg cgggagcaaa tcgccctgtt
    23461 gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat ttgatgttgc tggcggcggt
    23521 atatcagatt tctgactcaa tccaggtgat tggcagtggg attttgcgtg gttataaaga
    23581 tacgcgttcc attttctata tcacctttac ggcttactgg gtgctgggct tgccaagcgg
    23641 ctatattctg gcactgaccg atctggtcgt tgaacctatg gggccagcag gcttctggat
    23701 aggctttatt attggcctga cgtcggcagc cattatgatg atgctgcgta tgcggttcct
    23761 gcaacgtctg ccgtcagtta tcattctgca acgagcatcc cgctaataaa gacaaggcgc
    23821 aaccttcacg ggttgcgcct gtgtttttac gcaggctgga gcgttgcgcc aatcccgtct
    23881 tcgtctggct gtaatttcag agcgttgcac agagtttgcc gactggcatc tgttatctca
    23941 acgtgtatat ccccggtaac ttcccctttc acattgatga agtcaggtga gatttctttc
    24001 gcactggttt gcaggcaaat cgctccctct ccgcgcaggc gtttcacttt cagttgcaca
    24061 tgcgttgccg caccagtgat cagccacagt tttccgccat tcagattgat gttaccaaca
    24121 ataaaagtga caccgtcacc ttttaccgta ctgccgtcga gaatcaacac accgccctgc
    24181 tgtacagtaa tcgttgattg ttgcagatct gcgccatagc caataatcat tgaagcgccg
    24241 tccagtaatg caacactccc ggtcagttca accggtgcgc gatcttcctc tacgacaaaa
    24301 ttttcgtccg cgctggtggg tgacaacagg atcactgttc caccttgtat taccgcattt
    24361 tccagcgtac cggcatgcac ttcagcccga ccacccacat tcacggacag gttttgcgtc
    24421 ttaccggaac tgtagagctg aaggccatcc gtccccagac gataaatgga tccattctca
    24481 acaatagtgt cagagtcttc accaccgaga ttattcatgg tgccaccagc aagcaacgta
    24541 tcactggctt tcccggccag cataaaaacg ccgccctgat taatctcaca attgctggct
    24601 tccccacctg gatacaccaa caattcacca ccttcatcta ccacgaccgc tgaggttgtc
    24661 ccattaacca acaggccgcc ctcttgatcg agaatgattt tttccgcgcg atgtccttcc
    24721 agtacacgca ggttaccgcc attttccagc aacaaaccgc aggcataacc ctgatcaacg
    24781 ctgaattcac cttcgggatg gcggccatta accgtagcga gcgtagagag actaattgcg
    24841 cctccctcgg actgaacaat atctgtggca atggcaccgt cgctgaccga tagcgtgcca
    24901 ttgttaccgt actgtgtgca tttggcgatc ccaccggttg ccactttcaa ccagccagcc
    24961 cccgtgacca cggtattttc ggctcttccg ccagcttcaa caatcaactc gccttcagta
    25021 agttgcgtcg cgccaatgac ctcgccggtc ttaacaattt gcacattccc gtcgctcatc
    25081 aagtttttcg catcagatcc catgatttat tcctttgctg catctgtgtg cctttattgc
    25141 tacctaagtg taaaggctac ggaggattta tccacgacag atttgagatg gtggcaaaca
    25201 actctgttta aactctgata cacgaattat tgggttgtat cagatgtaaa tgcgatcctg
    25261 aataaaaatc gcccttgcaa atcaacaaaa tatagccaat ttgcttaaaa gctctgcaaa
    25321 cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc accttcccac tctgccgcta
    25381 atattcgtcc ccgttgtcac ctacaacgtt gcgttcatag ctcagttggt tagagcacca
    25441 ccttgacatg gtgggggtcg ttggttcgag tccaattgaa cgcaccatcc tgcgtccgta
    25501 gctcagttgg ttagagcacc accttgacat ggtgggggtc ggtggttcga gtccactcgg
    25561 acgcaccaga ttttcttaat ctggtcttct cctttttccc tctgtttctt ctctgtatcc
    25621 aatacgttaa aagatttaca ctgtcttcgt atgcgttatc agaaggagaa tcgctatggc
    25681 aactttgtta caacttcatt ttgcttttaa tggcccattt ggcgacgcaa tggctgagca
    25741 gcttaaaccg cttgctgagt cgattaatca ggaacctggt tttctgtgga aggtatggac
    25801 agaaagtgaa aagaaccacg aagccggtgg gatctatctt tttactgatg aaaaaagcgc
    25861 ccttgcctat ctggaaaaac atactgcccg acttaaaaat ctcggcgttg aggaagttgt
    25921 cgccaaagtt ttcgatgtca atgagccact gagtcaaatc aatcaggcaa aactcgcctg
    25981 acagaattta atcaagggcg gttagcgccc ttttcatccc tgtctgaaat ttctcaaatt
    26041 ctaaaaatct caaccaaact tatctgataa cactaaattc gaaagaatgc gtacaggtaa
    26101 gtaacaatga aaaaaattgc tattgtgggt gccgggccta cggggatcta caccttattc
    26161 tcgcttctac agcaacaaac tccactttct atttctatct tcgagcaggc tgacgaggcc
    26221 ggtgtcggga tgccatacag tgatgaggaa aactcaaaaa tgatgctggc aaatattgcc
    26281 agtattgaaa taccgccgat taattgtacg tatctcgaat ggctacaaaa gcaagaagcc
    26341 agccatctcc agcgttatgg cgttaaaaaa gaaaccttgc acgatcgtca gtttttaccg
    26401 cgaattctgc tgggcgaata tttccgcgat caatttttac gattagtaga ccaggcacga
    26461 cagcaaaaat ttgcagtggc tgtttatgaa tcatgccagg ttaccgatct gcaaattaca
    26521 aatgctggcg tcatgctcgc tacaaatcag gatttaccca gcgagacgtt tgatttagcg
    26581 gtgattgcca cgggtcacgt ctggcctgat gaagaagaag caaccagaac gtattttccc
    26641 agtccgtggt caggcctgat ggaagcaaag gtcgatgcgt gtaacgtggg tattatggga
    26701 acatccttga gcggactgga tgcggcaatg gcagtggcta ttcagcatgg ttcgttcatt
    26761 gaagatgata aacaacacgt cgtttttaac cgcgataacg caagtgaaaa gctaaatatt
    26821 acgttgatgt cgcgcacggg tattttaccc gaagccgatt tctattgccc tattccctac
    26881 gagcccttac acatcgtcac cgatcaggca ttaaatgctg agattcaaaa aggtgaagag
    26941 ggccttttgg atcgggtatt tagattgata gtagaggaaa tcaagtttgc tgatccagac
    27001 tggagccaac gcatagcctt agagagcctg aatgtcgatt cctttgctca agcctggttt
    27061 gccgagcgca aacaacgcga cccatttgac tgggcagaaa aaaatctcca ggaagtcgaa
    27121 cgcaataaac gagaaaaaca tactgttccc tggcgttatg tcattctgcg cctgcatgaa
    27181 gccgtacagg aaattgttcc tcatctgaat gaacacgacc ataaacggtt cagtaaaggc
    27241 cttgccaggg ttttcatcga taattatgcg gcaatccctt cagagtctat tcgtcgccta
    27301 cttgccttac gtgaagcggg aatcattcat attctcgccc tcggtgaaga ctacaaaatg
    27361 gaaattaatg agtcgcgcac cgtcctgaaa acggaagaca acagctactc gtttgacgtt
    27421 tttattgatg cccgcggaca gcgtccgctt aaagtgaaag atattccttt ccctgggcta
    27481 cgcgagcaat tacagaaaac aggggatgaa atccctgatg ttggcgaaga ttatacgtta
    27541 cagcaacccg aagatattcg tggacgcgta gcgttcggcg cgttgccctg gttgatgcac
    27601 gaccagcctt tcgttcaggg acttacggca tgtgcagaaa ttggtgaggc gatggctcgg
    27661 gcggtcgtaa agcctgcatc ccgtgctcgt cggcgtcttt cgtttgatta aaggtaaagc
    27721 tatcagcact caacaaactg atgctgttca ttaagacgat agcggactcc ggttcgaata
    27781 ttgttttcac cttcaatggc gacggcgaag cgaactcttt cgccgtcatg atatgccagt
    27841 gcggcgcttc cccctggccc gagaataatt gagtcgacca cgcccgtgct cactaccgtt
    27901 gaattttcac cgctggcaat gatgtgtaca ttgtcgccgc tgttggcaat tctggcgttt
    27961 gcgccaaagc tggcgatttg caccaggtca ccattactgg cgacatgaca ccgttcaccc
    28021 aatgtacaaa cgcgtactcg cattccggta ttcgcaatcc gactactatc tccagcgctg
    28081 cttatccgcg aagaattacc ggttacagca acccgtgcgc gttcaccgga gctgccaatg
    28141 tgactgttaa agccgacact tccaatccgc acgctataac ccatactggc aatttgcgcg
    28201 gcatacccag cggttgcgat tcgggcgtta tcctcacaac agccgatgcg agcatgatcg
    28261 tcgctgcggg caaactgact gcaaaatggt atctctccgg ggtctgtggc cagtttttgc
    28321 atagaggaaa cttcctgatg cgcgaagttt tcatccgcca gccatttttt ccacgcatac
    28381 tcaaccagac tctccgccca gtccgtatat ccttcctcaa tcagtgcatg atgaatatcg
    28441 gcataactac cgccgcgtgg gaaatggcgc aggaaccagc gatacatcac cgctccgata
    28501 cgccattctc gcaggtctgc tcgtgtaatg atcattcgtt agtgccggtg atagccatcg
    28561 accatgcttt taaaggtttc gtgtggcgta cgccccgtgg tgcaaagata aagatgaccg
    28621 aagataaaaa agaggcttat aaatgccaga gcaaaatgcg cctgcaataa ccagtatctt
    28681 acgccaggaa acacatctcc cacggcttgc ggatagagac acagcagccc cgtcaatagt
    28741 aacaacggca gcaatccata cataacacca acataggcga cctgctgtaa gggattaaat
    28801 ttagactggg ttgttgccgg gaaaggatgt tcttccccct gcataatgcc aaacaaataa
    28861 aatcgcgttt gttttgccgc tcgctccagc cacccctgac gacgaatgcg atagtggtga
    28921 ccattacccc caacggcatt gatcagcaca aagccgagcc agcatgccag taacaaaaat
    28981 ccgcaaactt catgcaccgc aaccagactt ttaaccgcag ttgcgcccac cagcgcaaag
    29041 tgatttatca gcccacttgc cagcaacaat acaaagagta acgcattcga ccagtgccat
    29101 aatctgaccg ctttgcagta aagataaatc ttctcaccat aatcggtgac tgatttcttc
    29161 acgccacgag cacgaagcaa ggcatgcagc cccagcacca accacatccc aaccagcaat
    29221 actccggcaa tgatcaacca caccggccaa aattcgggag tgaactgtgg cacatagttc
    29281 gctaactggc tctgaaactg ttcagcatgt tgcgacgggt tcatacattt tcctttttaa
    29341 tcaaatgttg accgaaccgg cgatacagat gcggtttccc ggcaccaggt agctgatatt
    29401 gatagtattt attttgctgc aaccacgcct gaatttctgg gctatcttcg cgaccaaaga
    29461 tcaatgcgtg ttccgggcag gcgctgacgc aaatgggcgg aaagcctttt gccagtcggg
    29521 actcagcgca gaaatcacat ttatctgcca ctttagtgac agggttaagg tagcgaacct
    29581 ggtacggaca agcgccaatg caataactgc agccgatgca ctgcgatttt tctacccgca
    29641 cgatcccctg ctcatcacgc cacgacgccc ctgtcgggca tacgtcgatg cacggagcat
    29701 cttcgcagtg ctgacatgac tggcgaaaaa agtgatactg cgtttcgttg tcgttatcgg
    29761 tgactggaat gtgtgctatc gataaacggc ttccctgagc ggggacgtgg ttagttttac
    29821 ggcatgcgcg ggcgcaaata ttacagccat tgcaccgtga ctcgtcatga atcatggcat
    29881 accgaacttc cttttcttgc ctcgtgttcg ctaacagaga agacgcagaa ccagtaaaaa
    29941 agattactgt tcccatcccc agaacaaatt tgcgtcgagt gaaggacatc tgattatccc
    30001 ctaatgcagt ggctgcaact gtccacccta tccatgacat ctccctgaaa tttcaggggt
    30061 tctgaaactg ctctacctcg aaccattcgc ataagccata agccttgcca taggtcataa
    30121 atcgctcgct aaacaagtac aacaccgccg tcgatgactc cacctgccgt atatcggcat
    30181 attcaggtgc aacatccatc gcggcgatcg cggcttcaat ctgcgcttcc tggaagtaat
    30241 atggcgcttg catcagcatg gcgactttgt aaggacgagg atatgtctgg cactcaaaac
    30301 gaacggcgtg tgcaatggcg cgacaaatgt cctgctccac aacttgcaaa gacatcgccg
    30361 cgtagttttc actcatggcc tgcgttgagt aatagtaatc atcctggctt cccgtcatac
    30421 aagcgatatc agcgtagtct ccgttctgct taatgctttc cagcaaaact gacagctcct
    30481 cttctgcaac cgaatagggt ggcgagagaa acacggcgcg cgccaccagc tgcccagccg
    30541 cagaatgctg gcgaatgaac tctgccagga tttcagccgg ttctggttcg gttaccgtat
    30601 caacggggtg acgcatcccc tgtaaccatt gccgcttcgc ctcgtcaact tcgctgactt
    30661 tcgctaacgg caattcatcc cgatggttca tctcacccat tccttacaca ggcagtagat
    30721 tgtgtgctgc cagatcggcg gcaatatctt ccagccccag acgctgcaat gtttcgcggg
    30781 tgggacaacc aagctgtgga tcccagccca tttctttgta gaacatcgtg agcgaagcat
    30841 gcatatcgtc acgatccatt ttgtcagtac cttcagtaaa gaccgggatc tgcggatcct
    30901 tgtcgaatac ccaggaacag ataagatcgt gttcattacg catatctttg gtttgcataa
    30961 gttttaccgt gtaggcacga tgcaacgtaa agatacgctc tgcagctaaa tctaattttt
    31021 cctgagtcat ctcttcgccg gtgatcgctt tgaagaattt ggcttcaagc gccagatcgc
    31081 cccggtaatt acggcttttc agcggcgaaa cggtcattgg ccagacccag ttgcacaggg
    31141 tgacggcgtt atgcaaacag acccgcaaca gcgaccactt tgcatattta atttttgcgt
    31201 cattgattgg cgtgtagttt ttggtttcat cgtaggcatc ttcagaacca aaaagctctt
    31261 tcgccacttc acgctgcagt ttcaatggca aacccgaacc aataaaattg atatgggtat
    31321 gcgtcatgca gtcacggttg aacatacagt taacaatgga accgacctgc gctgacgctt
    31381 cattggcatg gtgaaccgga tagccaaacg gcgaccagag tttatttttt gcgtagcccc
    31441 agtactcttc acccaaattc cagcgttctg cgatggcata tgaaccatca gccaggtgac
    31501 tcagctcacc cacacgatgc gccagacggt agtaaaaatc tttaatgaaa ttaacgtcac
    31561 ccgcttccag ttgatcccag tgaatttccg catactcttc agctggcaga acacgcttga
    31621 acaccccttt gctgtaacaa taagtaaagt cgcgatgcag ctgcccgtag ttacaccata
    31681 ggccgtagtc atcgaacaaa ttcagaccaa ccaggttacc aatcacacga ccatcgtctt
    31741 tatcttcaaa atctttcggc ccgttcggga agatggtggt atggacaaag ttggctacac
    31801 aagtgttacc acctgtgctg ggcacgccaa actctttcac ccgaggaata ttcatttggg
    31861 tcatacaacg gatcgggcaa gagtggcagc cactcatttt tactgtgtat ttctctgccg
    31921 ccggacctaa gtcaaaaacg gatttatagg tacgaaagcc gaccgtgttc tgattgcctg
    31981 gcggaatttc ccccgtttca atcggaccac cttcagccgc gccccaaaac agccctttac
    32041 gtgctgtcca acgtgacttg ggatctgaat actctgccca cgattgcggc gtacttggca
    32101 cgacatggtt gttattcgca ccaataagtt cagtcatcat gtaatcgttg agacgcttca
    32161 tctcctgacg atcggcaatg ttgacccctt tcgtcccttc aaccgcaatc gctttcaggt
    32221 ttttcgaacc cataattgcg ccagttcccg caccgccgct gtggttacgg ctattcaaca
    32281 tgccagagag aggaacaagg ttttccccag cctgaccaat agccgccaca caggtttccg
    32341 gactggtcaa tcgacaaatt tcttccgtcg ttgcgcgcgt cccttttccc cataagaaat
    32401 cggctttttc caggctaact ttgtcatctt taatcttcag ccataccggt gatttcgcct
    32461 tcccttcgat aataatgacg tcgtatccag cgaatttcat ttgcgccgca aaaaagcccc
    32521 ccatatgggc atcgacaact aaatttcctt tggtaaaagt agaaagtgag gtgatattta
    32581 cgcgagaact acagggggca ccagatccag ttaatgggcc ggtagcaaag actaatttat
    32641 tcgcttcatc gaaaggtttc gtgcctggcg gtacttcgtc atacataatt ttgtagccga
    32701 agcccatgcc accgacaaaa cttttaaact tactggaatc ttcgagggta atatttcctg
    32761 tcgtgagatt gactcttaat atattacctg tccaaccgtt agccatgatt ttttcctttg
    32821 caagattcac acagtaatat ctttccactc gataattttt aacgcccctg ttgggcaggc
    32881 gtttgcgcat tcaccgcata acacacattt cgaggatttt ttactttcgg tatttacggt
    32941 ggccatcatc cacgggcatg ccgtagtaca ggcgctacag ccaatacagc gtttatgatc
    33001 gacggtaata cagccttctt tctgctgcca ggtaatcgca ccaatcgggc agacgttcat
    33061 gcattgcggc tctttgcatt gacggcaggt gtccgcggta tagttgagat cgccatacag
    33121 gccgccgcca gagccaaccc cgttgtcgcc aaagaaataa ttgcgatgga ttttgatacg
    33181 ggagaagaat gtccctactg agccatcgtt gaagttggta caagagattt cacagcggtg
    33241 gcaacccgtg catcgcgctc gctgggtcac cagcacccct ttgggtgtat ttatcaaccc
    33301 tactgtgccg ctatcaatat cttcctgttt gcaacccaaa agtgacagta acgcaggggc
    33361 aattgttaga ccagcaaggc cttttcctga tatacgcaag aattctaatc gcgttaagcc
    33421 aatatctaat agtggacgat caaccgggtt catttatttc gcatcctctt cgcaaacaca
    33481 gtgctgacaa ttcagcagcc tgatattaag cattcaacta atacgctgct gactctgtcc
    33541 ccaacaccag atattttccc taacccgata gtgtatgaga aaagatttaa cgccgataaa
    33601 ctaatccttt atggttagga gaaaaaataa
//
LOCUS       DAPGWD010000036        32618 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPGWD010000036 DAPGWD010000000
VERSION     DAPGWD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.036"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(52..213)
                     /gene="rsmS"
                     /locus_tag="RHX88_001671"
     CDS             complement(52..213)
                     /gene="rsmS"
                     /locus_tag="RHX88_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDV2230367.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(227..754)
                     /gene="priC"
                     /locus_tag="RHX88_001672"
     CDS             complement(227..754)
                     /gene="priC"
                     /locus_tag="RHX88_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDV2230368.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVEQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            824..1201
                     /locus_tag="RHX88_001673"
     CDS             824..1201
                     /locus_tag="RHX88_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDV2230369.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            1354..1905
                     /gene="apt"
                     /locus_tag="RHX88_001674"
     CDS             1354..1905
                     /gene="apt"
                     /locus_tag="RHX88_001674"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDV2230370.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            2034..3965
                     /gene="dnaX"
                     /locus_tag="RHX88_001675"
     CDS             2034..3965
                     /gene="dnaX"
                     /locus_tag="RHX88_001675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDV2230371.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    3298..3362
                     /gene="dnaX"
                     /locus_tag="RHX88_001675"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            4018..4347
                     /locus_tag="RHX88_001676"
     CDS             4018..4347
                     /locus_tag="RHX88_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDV2230372.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            4347..4952
                     /gene="recR"
                     /locus_tag="RHX88_001677"
     CDS             4347..4952
                     /gene="recR"
                     /locus_tag="RHX88_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDV2230373.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            5062..6936
                     /gene="htpG"
                     /locus_tag="RHX88_001678"
     CDS             5062..6936
                     /gene="htpG"
                     /locus_tag="RHX88_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDV2230374.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            7117..7761
                     /gene="adk"
                     /locus_tag="RHX88_001679"
     CDS             7117..7761
                     /gene="adk"
                     /locus_tag="RHX88_001679"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDV2230375.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            7997..8959
                     /gene="hemH"
                     /locus_tag="RHX88_001680"
     CDS             7997..8959
                     /gene="hemH"
                     /locus_tag="RHX88_001680"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDV2230376.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8956..9915)
                     /gene="aes"
                     /locus_tag="RHX88_001681"
     CDS             complement(8956..9915)
                     /gene="aes"
                     /locus_tag="RHX88_001681"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDV2230377.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            10067..11371
                     /gene="gsk"
                     /locus_tag="RHX88_001682"
     CDS             10067..11371
                     /gene="gsk"
                     /locus_tag="RHX88_001682"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDV2230378.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(11454..13130)
                     /locus_tag="RHX88_001683"
     CDS             complement(11454..13130)
                     /locus_tag="RHX88_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDV2230379.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(13368..14588)
                     /gene="fsr"
                     /locus_tag="RHX88_001684"
     CDS             complement(13368..14588)
                     /gene="fsr"
                     /locus_tag="RHX88_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDV2230380.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            14806..16458
                     /gene="ushA"
                     /locus_tag="RHX88_001685"
     CDS             14806..16458
                     /gene="ushA"
                     /locus_tag="RHX88_001685"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDV2230381.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(16495..16974)
                     /gene="ybaK"
                     /locus_tag="RHX88_001686"
     CDS             complement(16495..16974)
                     /gene="ybaK"
                     /locus_tag="RHX88_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDV2230382.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17178..17972)
                     /locus_tag="RHX88_001687"
     CDS             complement(17178..17972)
                     /locus_tag="RHX88_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDV2230383.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            18110..18451
                     /locus_tag="RHX88_001688"
     CDS             18110..18451
                     /locus_tag="RHX88_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV2230384.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(18565..21069)
                     /gene="copA"
                     /locus_tag="RHX88_001689"
     CDS             complement(18565..21069)
                     /gene="copA"
                     /locus_tag="RHX88_001689"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDV2230385.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            21331..22263
                     /gene="glsA"
                     /locus_tag="RHX88_001690"
     CDS             21331..22263
                     /gene="glsA"
                     /locus_tag="RHX88_001690"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDV2230386.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            22266..23558
                     /locus_tag="RHX88_001691"
     CDS             22266..23558
                     /locus_tag="RHX88_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2230387.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            23683..24090
                     /gene="cueR"
                     /locus_tag="RHX88_001692"
     CDS             23683..24090
                     /gene="cueR"
                     /locus_tag="RHX88_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDV2230388.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(24091..24549)
                     /locus_tag="RHX88_001693"
     CDS             complement(24091..24549)
                     /locus_tag="RHX88_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDV2230389.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(24546..25463)
                     /locus_tag="RHX88_001694"
     CDS             complement(24546..25463)
                     /locus_tag="RHX88_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDV2230390.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            25609..26286
                     /gene="fetA"
                     /locus_tag="RHX88_001695"
     CDS             25609..26286
                     /gene="fetA"
                     /locus_tag="RHX88_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDV2230391.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            26273..27052
                     /gene="fetB"
                     /locus_tag="RHX88_001696"
     CDS             26273..27052
                     /gene="fetB"
                     /locus_tag="RHX88_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDV2230392.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(27115..27969)
                     /gene="cnoX"
                     /locus_tag="RHX88_001697"
     CDS             complement(27115..27969)
                     /gene="cnoX"
                     /locus_tag="RHX88_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDV2230393.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(28030..28839)
                     /gene="ybbO"
                     /locus_tag="RHX88_001698"
     CDS             complement(28030..28839)
                     /gene="ybbO"
                     /locus_tag="RHX88_001698"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV2230394.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(28829..29452)
                     /gene="tesA"
                     /locus_tag="RHX88_001699"
     CDS             complement(28829..29452)
                     /gene="tesA"
                     /locus_tag="RHX88_001699"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDV2230395.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            29423..30109
                     /locus_tag="RHX88_001700"
     CDS             29423..30109
                     /locus_tag="RHX88_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2230396.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            30106..32520
                     /locus_tag="RHX88_001701"
     CDS             30106..32520
                     /locus_tag="RHX88_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2230397.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLKVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         7734 a         8612 c         8793 g         7479 t
ORIGIN      
        1 ttttgcgttt gcccacaatc ttaggggaca tttagcggcc ccatttattt ctcacttttc
       61 cgcctcatca tcgcgcgtta atttcttttc ataatcgcgt tttacaatat ccagcgcgcg
      121 cagcacggtg cgggctggga tctgattttc ttccagcagc acaatcaaat cgacagccag
      181 tttgacatcg tcaggggcat tttccagtga catactctct ccattgctag cgggttaagc
      241 gcgctagcct gttttcgatt ttttccagcg catggcggca gcgtgccagg cgcgcttcat
      301 aggcttccac ttcacgatgc agcgtttgct gttccacgag atcggtcacc cgcgccaggc
      361 gggctctgcg ttcggcaacc atctcacgta gccgccgctc aaaatcctga tgctgaatac
      421 gtttacgctg ccagcgggca attttcggtg gcgcactgtc ccactcgcgc aatgaccagg
      481 cggaggcttc acgcgcgatg gcttccagtt gtgccgccag atgttccgcc agccaggcca
      541 cttgcggcag ctgttgctgc tcaactgcat gacgaagcgc agccagatta tcgcccgcct
      601 catcgagaca agcctgcagt gttgtcgcac gggtttgaaa aaggtgtctg tcaaaacggg
      661 cacttagcgt ggcaaattgc tctaccgggg cacaacgctg acgcagcgta gcgagctgtc
      721 cttccagttt ttccagcagc agggcggttt tcacgataag acctcaatga aaatgataat
      781 tgttatgcta aagtagccac tctgtaagct gacaacactc aatatgcaac gaatcatttt
      841 aatcatcatt ggctggctgg cggtagtgct gggtacgctg ggcgtggtat taccggtatt
      901 accgacgacg ccgtttatcc tgctggcggc ctggtgcttt gcccgttctt ccccgcgctt
      961 tcacgcctgg ttgctgtacc gctcatggtt tggcagctat ctacgtttct ggcagaaaca
     1021 tcatgcgatg ccgcgcggcg tcaaaccgcg ggcgattttg cttattttgc tcacgtttgc
     1081 catttctctg tggttcgtcc agatgccatg ggtgcgcatc atgttgctgg taattctcgc
     1141 ctgtttgctt ttctatatgt ggcgaattcc ggtgattgat gaaaagcaag aaaagcactg
     1201 aagcacaaca atcgcagttg caattattgc gtacagccag tacattctgg cgttttcgag
     1261 cacaggcgca ggcggtcaaa ggttaaacaa ctgttacttt tgatacgttt aaaacgcgcc
     1321 gtgagtacca ccgtaacaag caggcataca cttatgaccg cgactgcaca gcagcttgag
     1381 tatctcaaaa atagcatcaa aagcattcag gactacccaa aacccggcat tcttttccgc
     1441 gatgtcacca gcttactgga agacccgaaa gcttacgctc tcagcatcga cttgctggtt
     1501 gagcgttaca aaaatgcggg cattaccaaa gttgtcggca ccgaagcgcg tggcttcttg
     1561 tttggcgctc cggtagctct gggtctgggc gttggctttg taccggtccg taaaccgggc
     1621 aaactgccgc gtgaaaccat cagtgaaact tacgacctgg aatacggcac cgatcagctg
     1681 gagatccacg ttgatgccat caaaccgggc gacaaagttc tggtggtgga cgatctgctg
     1741 gcaaccggcg gcactatcga agcgaccgtt aaactgatcc gtcgtctggg tggtgaagtg
     1801 gctgacgctg cgttcattat caacctgttc gatctcggcg gcgaacagcg tctcgaaaaa
     1861 cagggcatta ccagctacag ccttgtcccg ttcccgggcc attaattatc gccagtcttg
     1921 tgctgcccac gctacggaca gcacaagatg tgcattcagc ctcgccgttc tgacggggct
     1981 gtgttagcat taccccttcg tgaatccacc ttccagcgtt tcagagcctg ccaatgagtt
     2041 atcaggtctt agcccgaaaa tggcgcccac aaacctttgc tgacgtcgtc ggccaggaac
     2101 atgtgctgac cgcactggcg aacggcttgt cgttagggcg tattcatcat gcttatcttt
     2161 tttccggtac ccgtggcgtc ggaaaaacct ctatcgcccg actgctggcg aaggggctaa
     2221 actgcgaaac cggcattacc gcgacgccgt gcggcgtgtg cgataactgt cgtgaaatcg
     2281 agcaggggcg ctttgtcgat ctgattgaaa tcgacgccgc ctcgcgcacc aaagttgaag
     2341 atacccgcga cctgctggat aacgtccagt acgctccggc gcgtggtcgt ttcaaagttt
     2401 atctgatcga cgaagtgcat atgctgtcgc gccacagctt taacgcactg ttaaaaaccc
     2461 ttgaagagcc gccggagcac gttaagtttc tgctggcgac gaccgatcca cagaaattgc
     2521 cggtgacgat tttgtcgcgc tgtttgcaat ttcatctcaa ggcgctggat gtcgagcaaa
     2581 ttcgccatca acttgagcac atcctcaacg aagaacatat cgctcacgag ccgcgggcgt
     2641 tgcaattgct ggcgcgcgcc gctgaaggca gcctgcgaga tgccttaagt ctgaccgacc
     2701 aggcgattgc cagcggtgac ggccaggttt caacccaggc agtcagtgcg atgctgggta
     2761 cgcttgacga cgatcaggcg ctgtcgctgg ttgaagcgat ggtcgaggcc aacggcgagc
     2821 gcgtaatggc gctgattaat gaagccgctg cccgtggtat cgagtgggaa gcgttgctgg
     2881 tggaaatgct cggcctgttg catcgtattg cgatggtaca actttcgcct gctgcacttg
     2941 gcaacgacat ggccgccatt gagctgcgga tgcgtgaact ggcgcgcacc ataccgccga
     3001 cggatattca gctttactat cagacgctgt tgattggtcg caaagaatta ccgtatgcgc
     3061 cggaccgccg catgggcgtt gagatgacgc tgctgcgcgc gctggcgttc catccacgta
     3121 tgcctctgcc tgagccagaa gtgccacgcc agtcctttgc gcccgtcgcg ccaacggcag
     3181 taatgacgcc aacccaggtg ccgccgcaac cgcaatcagc gccgcagcag gcaccgactg
     3241 taccgctccc ggaaaccacc agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc
     3301 agggagcaac caaagcaaaa aagagtgaac cggcagccgc tacccgcgcg cggccggtga
     3361 ataacgctgc gctggaaaga ctggcttcgg tcaccgatcg cgttcaggcg cgtccggtgc
     3421 catcggcgct ggaaaaagcg ccagccaaaa aagaagcgta tcgctggaag gctaccactc
     3481 cagtgatgca gcaaaaagaa gtggtcgcca cgccgaaggc gctgaaaaaa gcgctggaac
     3541 atgaaaaaac gccggaactg gcggcgaagc tggcggcaga agccattgag cgcgacgcgt
     3601 gggcggcaca ggtgagtcaa ctttcgctac caaaactggt cgaacaggtg gccttaaatg
     3661 cctggaaaga ggagagcgac aacgcagtat gtctgcattt gcgctcctct cagcggcatt
     3721 tgaacaaccg cggtgcacag caaaaactgg ctgaagcgtt gagcacgtta aaaggttcaa
     3781 cggttgaact gactatcgtt gaagatgata atcccgcggt gcgtacgccg ctggagtggc
     3841 gtcaggcgat atacgaagaa aaacttgcgc aggcgcgcga gtccattatt gcggataata
     3901 atattcagac cctgcgtcgg ttcttcgatg cggagctgga tgaagaaagt atccgcccca
     3961 tttgatcgta agcacagctt acgttcgtca tccttaacgt gattgagaga gaaacctatg
     4021 tttggtaaag gcggtctggg taacctgatg aagcaagccc agcagatgca agaaaaaatg
     4081 cagaaaatgc aggaagagat cgcgcagctg gaagtcaccg gcgaatctgg cgcaggtctg
     4141 gtaaaagtga ccatcaacgg tgcacacaac tgccgtcgcg tagagatcga cccgagcctg
     4201 ctggaagacg acaaagagat gctggaagac ctggtggctg cagcattcaa cgacgcagca
     4261 cgtcgtattg aagaaacgca gaaagaaaaa atggcctctg tatcctccgg aatgcagctg
     4321 ccgcctggct ttaagatgcc gttctgatgc aaaccagccc gctgttaaca cagcttatgg
     4381 aagcactgcg ctgtctgccg ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc
     4441 tgcttcagcg cgatcgtagc ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt
     4501 cggaaatcgg ccactgcgcc gattgccgca ctttcaccga acaggaagtc tgtaacatct
     4561 gttcgaatcc gcgtcgtcag gaaaacggtc aaatctgtgt ggtggagagt ccggcggaca
     4621 tctacgccat tgagcagacg gggcagtttt caggccgtta ttttgtgttg atggggcacc
     4681 tgtcaccgct ggacggcatc ggtccggatg atatcggcct tgatcgtctg gaacagcgtc
     4741 tggcagagga aaaaatcact gaagtgatcc tcgccaccaa tcccacggtt gaaggtgaag
     4801 ctaccgctaa ctacattgcc gagctttgcg cgcaatatga cgtggaagcc agccgaatcg
     4861 ctcatggcgt accggttggc ggcgaactgg aaatggtcga tggcaccacg ctgtcacact
     4921 cccttgccgg gcgtcataag attcgttttt aagcaaacga gagcaggatc acctgctctc
     4981 gcttgaaatt attctccctt gtccccatct ctccctcatc ctgtttttaa ccttaaaatg
     5041 gcattattga ggtagaccta catgaaagga caagaaactc gtggttttca gtcagaagtg
     5101 aaacagcttc tgcacctgat gatccattct ctctattcca ataaagaaat cttcctgcgt
     5161 gagcttatct ctaacgcctc cgatgcggcg gacaagctgc gtttccgtgc gctatctaac
     5221 ccggacctgt acgaaggtga tggcgaactg cgcgttcgtg tctctttcga taaagacaag
     5281 cgcacgctga ccatctccga taacggcgtg gggatgaccc gcgacgaagt gattgaccat
     5341 ctggggacta tcgctaaatc cggtaccaaa tcattcctcg aatccctggg ttctgaccag
     5401 gcgaaagaca gccagctgat cggtcagttt ggtgttggtt tctactctgc gtttatcgtg
     5461 gccgacaaag tgaccgtgcg tactcgcgcg gcaggcgaaa aaccagaaaa cggcgtcttc
     5521 tgggaatcgg ctggcgaagg tgaatatacc gttgccgaca tcaccaaaga agatcgtggt
     5581 actgaaatca ccctgcatct gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg
     5641 cgttccatca tcagcaaata ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc
     5701 gaagagaaag acggcgaaac cgttatctcc tgggagaaaa tcaacaaagc gcaggcgctg
     5761 tggactcgta acaagtcgga aatcaccgat gaagagtaca aagagttcta caaacacatc
     5821 gcccacgact ttaatgatcc gctgacctgg agccacaacc gcgttgaagg taagcaggag
     5881 tacaccagcc tgctgtacat cccgtcccag gctccgtggg atatgtggaa ccgtgatcat
     5941 aaacacggcc tgaaactgta cgtccagcgt gtgttcatca tggacgacgc agaacagttc
     6001 atgccgaact atctgcgctt cgtgcgtggt ctgattgact ccagcgatct gccgctgaac
     6061 gtttcccgtg aaatcctcca ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc
     6121 aagcgtgtgc tgcaaatgct ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc
     6181 ttctggcaac agtttggcct ggtactgaaa gaaggtccgg cggaagattt cgctaaccag
     6241 gaagcgatcg ccaaactgct gcgttttgct tctacttata ccgattcttc tgcgcagacc
     6301 gtgtctctgg aagactacgt ttcccgcatg aaagaagggc aggagaaaat ctactacatc
     6361 actgcagaca gctatgcggc agcgaagagc agcccgcacc tggaactgct gcgtaagaaa
     6421 ggcatcgaag ttctgctgct ttccgaccgc atcgatgagt ggatgatgaa ctatctgact
     6481 gagttcgacg gtaaaccgtt ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg
     6541 gctgacgaag ttgatgagag cgcgaaagaa gcggagaaag cactgactcc gttcatcgac
     6601 cgtgtgaaag ccctgctcgg cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc
     6661 gatacgccag cgatcgtttc gaccgacgcg gacgaaatga gcactcagat ggcgaaactg
     6721 ttcgctgcgg cgggccagaa agtgccagaa gtgaaataca tcttcgaact gaacccggat
     6781 cacgtactgg tgaaacgtgc ggcagatact gaagatgaag cgaagttcag cgagtgggta
     6841 gaactgctgc tggatcaggc gctgctggca gaacgcggca cgctggaaga tccgaacctg
     6901 tttattcgtc gtatgaacca gctgctggtt tcctgatgtc atgccggatg acctttgtgt
     6961 catccggcat ttttcttttc atcatctgca ctttccgcaa attatctcgc cattaaccgt
     7021 ttcagcctca ggtgcctttc ttgaggcaat cgcctgttgg tggtatcgtt tattgctttt
     7081 tcaaaaaatt cgacacattt taaggggatt ttcgcaatgc gtatcattct gcttggcgct
     7141 ccgggcgcgg ggaaagggac tcaggctcag ttcatcatgg agaaatatgg tattccgcaa
     7201 atctccactg gcgatatgct gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa
     7261 gcaaaagaca ttatggatgc tggcaaactg gtcaccgacg aactggtgat cgcgctggtt
     7321 aaagagcgca ttgctcagga agactgccgt aatggtttcc tgttggacgg cttcccgcgt
     7381 accattccgc aggcagacgc gatgaaagaa gcgggcatca atgttgatta cgttctggaa
     7441 ttcgacgtac cggacgaact gattgttgat cgtatcgtag gccgccgcgt tcatgcgccg
     7501 tctggtcgtg tttatcacgt taaattcaat ccgccgaaag tagaaggcaa agacgacgtt
     7561 accggtgaag aactgactac ccgtaaagac gatcaggaag aaaccgtgcg taaacgtctg
     7621 gttgaatacc atcagatgac tgcaccgctg atcggctact actccaaaga agcggaagcg
     7681 ggtaacacca aatacgcgaa agttgacggc accaagccgg ttgctgaagt tcgcgctgat
     7741 ctggaaaaaa tcctcggcta attcgaaagc gcgcacggac agtcccctcg ccccctcggg
     7801 gagagggtta gggtgagggg aacaggcccg cacaagcaaa cttatcagca atctcaggcc
     7861 ggatattcat tcggcctttt acaaaaacct caatctgcgc tattcctttt tctgatttga
     7921 cctctcacag caattagttc ttcttcctca cttttccgct acaattatca acaagttgaa
     7981 tcgataagag gcggtaatgc gtcagactaa aaccggtatc ctgctggcaa acctgggtac
     8041 gcccgatgcc cccacacctg aagcggtaaa acgctatctg aaacaatttt taagcgacag
     8101 acgcgtggtt gatacctcac ggttgttatg gtggccgttg ctgcgcggcg tgattttgcc
     8161 gctgcgctcg ccgcgtgtgg cgaagctgta cgcctcggtc tggatggaag acggctcgcc
     8221 gctgatggtt tacagccgcc agcaacagca ggcgttggca caacgtttac cggatacgcc
     8281 cgtagcgctg ggcatgagct acggctcgcc atcactggaa agcgccgtcg atgaactcct
     8341 ggcagagcat gtagatcata ttgtggtgct gccgctttat ccgcaattct cctgttctac
     8401 ggtcggtgcg gtatgggatg aactggcacg cattctggcg cgcaaacgta gcattccggg
     8461 gatatcgttt attcgtgatt acgccgataa ccacgattac attaatgcac tggcgaacag
     8521 cgtacgcgct tcttttgcca aacatggcga accggatctg ctgctgctct cttatcatgg
     8581 cattccccag cgttatgcag atgaaggcga tgattacccg caacgttgcc gcacaacgac
     8641 tcgcgaactg gcttccgcac tggggatggc accggaaaaa gtgatgatga cctttcagtc
     8701 gcgctttggc cgggaaccct ggctgatgcc ttataccgac gaaacgctga aaatgctcgg
     8761 agaaaaaggc gtaggtcata tacaggtgat gtgcccgggc tttgctgcgg attgtctgga
     8821 gacgctggaa gagattgccg agcaaaaccg tgaggtcttc ctcggtgccg gcgggaaaaa
     8881 atatgaatat attccagcgc ttaatgccac gccggaacat atcgaaatga tggctaatct
     8941 tgttgccgcg tatcgctaaa gctgagcggt aaagaactga gcgccgtcgc gaagagcctc
     9001 gtcggcggtt ttcatcattc gtgaataatg caaaaaggca tgcagcgtac ctgggtagag
     9061 tttgaactca cagggctgct gatgcgccgc taacgtctgg taaagcagac ggctgtcatc
     9121 cagcagcgga tcgaactccg ccccggcaat aaaacagggc ggaacttcgc gagtgagatc
     9181 attattaaac agacagtaat acggtgactc gcggtccgcg tcgttgctta aatacgcctc
     9241 ttcatacatc tgcaaatcct gttgcgttaa gccatcccag acaccgccca acagacgacg
     9301 agtcacggaa tcccgtaatc cgtaaagccc ataccacagc aaaacacccg caattttacc
     9361 gcaatcgatc tgtttatcac gcaaccacaa cgcactggcg agcgccagca tggcacctgc
     9421 ggaatcaccg gcaaagccaa tgcgggacat attgatttga taatcctccg cctgctggtg
     9481 gaaataacaa caagcagcca caatttcctc tatcgcttgc ggaaaacgcg cttcaggtga
     9541 aagggggtaa tcaataccaa tcaccgtaca ttggctgtag cttgccagca ggcgcatgat
     9601 gcgatcgtgg gtatcgagat tgccgagaat aaaaccgcct ccatgcaaat aaaatagcgt
     9661 cgccgggcta tctggctgcg gacaaaagag acgtgtttcc acctgcccat attttgttgg
     9721 aaccatgtaa gctctggttg ccatttctgg agcgcccgca ttccagaatc ggcgctcaag
     9781 cgtgtaatac tgtcgttgct cagcaatcgt tcccgttgcg ggccaggacg gtaaatccgg
     9841 ctgaagagta ttcacaacgg tcttcatttc agcagaaata aggtccagaa caggtagttt
     9901 gttttccggc ttcatacaaa aactcctttc aaattacgtc attgtaagga accactgcca
     9961 tgaaaatgcg atcccgcctg ctgatattga aactggctgc gtctcgcgcg ctcccgtcag
    10021 attgtgttaa cattcgccgc tcagttaacc acccgtaaaa acaaccatga aatttcccgg
    10081 taaacgtaaa tccaaacatt acttccctgt aaatgcacgc gatccgctgc ttcagcaatt
    10141 ccagccagaa aacgaaacca gcgccgcctg ggtcgtaggt attgaccaga cgctggtcga
    10201 tattgaagcg aaagtggatg atgaattcat tgagcgttat ggattaagcg ccgggcattc
    10261 actggtgatt gaggatgatg tagccgaagc gctttatcag gaactaaaac agaaaaacct
    10321 gattacccat cagtttgcgg gtggcaccat tggtaacacc atgcataact actcggtgct
    10381 cgcggacgac cgttcggtgc tgctgggcgt catgtgcagc aatattgaaa ttggcagcta
    10441 tgcctatcgt tacctgtgta acacctccag ccgtaccgat cttaactatc tacaaggcgt
    10501 ggatggcccg attggtcgtt gctttacgct gattggcgag tccggggaac gtacctttgc
    10561 tatcagccct ggccacatga accagctgcg ggctgaaagt attccggaag atgtgattgc
    10621 cggagcctcg gctctggttc tcacctctta tctggtgcgt tgcaagccgg gtgaacccat
    10681 gccggaagca accatgaaag ccattgagta cgcgaagaaa tataacgtac cggtggtgct
    10741 gacgctggga actaagtttg tcattgccga gaatccgcag tggtggcagc aattcctcaa
    10801 agaccacgtc tctatccttg cgatgaacga agatgaagcc gaagcgttga ccggagaaag
    10861 cgatccgttg ttggcatctg acaaggcgct ggactgggta gatctggtgc tgtgcaccgc
    10921 cgggccaatc ggcttgtata tggcgggctt taccgaagac gaagcgaaac gtaaaaccca
    10981 gcatccgctg ctgccgggcg ctatagcgga attcaaccag tatgagttta gccgcgccat
    11041 gcgccacaag gattgccaga atccgctgcg tgtatattcg cacattgcgc cgtacatggg
    11101 cgggccggaa aaaatcatga acactaatgg agcgggggat ggcgcattgg cagcgttgct
    11161 gcatgacatt accgccaaca gctaccatcg tagcaacgta ccaaactcca gcaaacataa
    11221 attcacctgg ttaacttatt catcgttagc gcaggtgtgt aaatatgcta accgtgtgag
    11281 ctatcaggta ctgaaccagc attcacctcg tttaacgcgc ggcttgccgg agcgtgaaga
    11341 cagcctggaa gagtcttact gggatcgtta agttatcgac cagaccggac gacgatggca
    11401 tccgtaggcc ggataagatg cgctagcatc gcatccggca ataacgtctt gttttacccc
    11461 gtcaccacct cacctgccgg tggcgtttcc agcaattcca gcatagtacg ggcaatttca
    11521 cgttcaccca tcactacctg attcgcgcca cgttcggtga tatacgtcac ttcatcgtca
    11581 taatgggcgc gggcgataat ctcaatatcc gggtttttcg cgcgggcaga cgccacaatc
    11641 tcacccgctt cataaccgtt ggggatcgtc aggatcagcc attttgcaca ttccagatgc
    11701 gccagttgca taatttcttc gttcgctgca ttgcccaata cagcgcggac cccacgctct
    11761 cgcagctcat caacacgggt tcgtgacgtc tcaatcacca ccagcggaat atcagaggcg
    11821 agcaatttct cccccagcag gctgcctaca cgaccgtaac ccaccaatag cgcatggttg
    11881 caaatatcca ctgggatctg tttctcttct tcgattgcct cttccagcgt ctgctcttcc
    11941 agcgtttcgg tcttcgccag atatttctcc agtagtgcga acagtaccgg gttgagcata
    12001 atcgacagga tcgcccctgc cagtaccagg ttttgtccgg cctgcggcag taaattcaat
    12061 gccattccca gtcccgccag gataaacgcg aactcaccaa tctgcgccag gctggcggcg
    12121 atggttaatg ccgtacgttg ggagtgacca aacagtcgca ccaggaaaaa tgcggctaac
    12181 gacttaccaa acagaataat cgccagcgtc gccagcactg ccagcggttg ctgaatcaga
    12241 attaacggat caaacaacat cccgacggag acaaaaaaca gcaccgcaaa cgcgtcgcgc
    12301 aatggcagcg tatcgtgggc ggcacggtga ctcagttcag actcgttcag taccatcccg
    12361 gcaaagaacg caccgagtgc aaaggagaca tcaaacagct ctaccgcacc aaaggcaatc
    12421 cctaacgcca gcgccagcac cgacagggta aacagctcgc gagaaccggt tgccgcgctg
    12481 cgtgccataa tccacggcac cagacggcga cctaccagca tcataatggc gataaatgcg
    12541 atcactttgc cgatggtgat ccccatatcg actgcaagag tggcaaagcc cacatcgccc
    12601 tgttccatca ttcctgccac tgcgggcagc aacaccagcg tcagaaccat taccaggtct
    12661 tccacaatca accaaccgat ggcgatttgc ccacgctgac tgtcaattaa ttgccgttct
    12721 tcaagtgcgc gcaataacac cacggtactg gcggtggaaa gacataaacc gaacacgata
    12781 ccggtcatta acgaccagcc cagcacggca gagagcgcca tacccagcag cgtcgccacg
    12841 gctatctggg cgatcgcacc gggaatggcg atggccttta ccgccatcaa atccttcagc
    12901 gaaaagtgca aaccgacgcc aaacatcaac agaatgacgc ccagttcagc cagttccggc
    12961 gcaagcttgg tatcggcaac aaagcccgga gtgaatggtc ctgccagcac acccgctaac
    13021 agatatccca ccagaggaga aatacgtagt ttattggcca gcatgccgag gataaaggcg
    13081 agcacaaggc cgccaacaat ggtggtgata agcggggtgg cgtgatgcat tccgtctcct
    13141 tttcctggtg gttattgtcc atttttggcc gggaaaacca aaattacagg taatagttta
    13201 tgacaatttc attgatgatg ttcatgaata attattgaat tttgcagaaa aatggaatta
    13261 gctgcaagaa aagcacggat cggaaaacag aaggcggttt aacagaggaa aggcttatgg
    13321 cgactgtaaa acgatgcagc caaagtttgc ctttggctgc aatgaaatca gtctttatgc
    13381 cggttatcag gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc acagatttta
    13441 tagactaact cgatgctggt gtgatcggcg ataagcccca gaactgccgc tcccagacct
    13501 cccatgccaa aagcaaaacc gaaaaagagt ccagaaacca taccgatacg tcccggaagt
    13561 agctcctgag cgtagaccag aatggcagag aatgccgaag cgaggataaa tccaataatc
    13621 accgttaaaa cccccgtcca gtgcaggctg gcgtagggta aaatcagcgt aaacggcgca
    13681 acgccgagga tagagcccca aatcacatat ttccgtccaa ttttatcccc tacaggcccg
    13741 ccgatcaccg tacctgccgc aacggcaaac aggaaggcaa acagatgaag ctgggcattc
    13801 tggatagata atccgaattt ttgcatcaga taaaaggtgt aatagctgct gatgctcgcc
    13861 atatagaaat atttcgagaa aatgaggatt aacagaatgc tgactgccag taccactttg
    13921 ttgcgcggca gtggattgat aatcgtcgct ttgggttttc ctttattcat tcggtgctgt
    13981 gccgagtacc aacggctgat ttgcgccaac accacgatcg ccagcagtgc cgcaagcaca
    14041 aaccaggcaa cgttgccttt gccataaggc gcgataatca ccgccgccag caagggtccc
    14101 agggaactgc caaagttgcc gccgacctga aagatagatt gcgccaggcc atgccgcccg
    14161 ccggaagcca tacgggctac gcgagaagat tccggatgaa agaccgatga accggtaccg
    14221 accagcgccg ccgccagcag aactgcgcca aaactgcccg ccagcgcaag cagcaccaga
    14281 ccacttaagg taaagcacat gccaattggc aacgaccacg gcatcggata tttatcggtc
    14341 cagtagccga ccactggttg cagtagcgaa gaggcgagct ggaaggtgag ggttatcatg
    14401 ccaatctgca taaatgtcag agaaaattct gactgaagca gcggataaat cgccagaatc
    14461 agcgattgga tcatgtcgtt cagcagatgt gagaggctga tagcacctaa aataccaaac
    14521 gatgttcggg ccttggtcgt tgacgcagcc gcgcccgcca caggctgggg ttgttcactc
    14581 attgccatag gaaagtcact ttttcagggt tgcgatgtaa agaatgatct tatttgtgat
    14641 tattacccga ctaacatacc tgtatgcgtc gcctgaagga agtctcaacg ccgaatacag
    14701 aatttctaat ctggatgcag atttatcttc accggacgca gacttgtcta tgatgtcgcg
    14761 tcatactatt tttcaacacg ttgaaatcag gtcagggaga gaagtatgaa attattgcag
    14821 cggggcgtgg cgttagcgct gttaaccaca tttacactgg cgagtgaaac tgctctggcg
    14881 tatgagcagg ataaaaccta caaaattaca gttctgcata ccaatgatca tcatgggcat
    14941 ttttggcgca atgaatatgg cgaatatggt ctggcggcgc aaaaaacgct ggtggatggt
    15001 atccgcaaag aggttgcggc tgaaggcggt agcgtgctgc tactttccgg tggcgacatt
    15061 aacactggcg tgcccgagtc tgacttacag gatgccgaac ctgattttcg cggtatgaat
    15121 ctggtgggct atgacgcgat ggcgatcggt aatcatgaat ttgataatcc gctcaccgta
    15181 ttacgccagc aggaaaagtg ggccaagttc ccgttgcttt ccgcgaatat ctaccagaaa
    15241 agtactggcg agcgcctgtt taaaccgtgg gcgctgttta agcgtcagga tctgaaaatt
    15301 gccgtcattg ggctgacaac cgatgacaca gcaaaaattg gtaacccgga atacttcact
    15361 gatgtcgaat ttcgtaagcc cgccgatgaa gcgaagctgg tgattcagga gctgcaacag
    15421 acagaaaagc cagacattat tatcgcggcg acccatatgg ggcattacga taatggtgag
    15481 cacggctcta acgcaccggg cgatgtggag atggcacgcg cgctgcctgc cggatcgctg
    15541 gcgatgatcg tcggtggtca ctcgcaagat ccggtctgca tggcggcaga aaacaaaaaa
    15601 caggtcgatt acgtgccggg tacgccatgc aagccagatc aacaaaacgg catctggatt
    15661 gtgcaggcgc atgagtgggg caaatacgtg ggacgggctg attttgagtt tcgtaatggc
    15721 gaaatgaaaa tggttaacta ccagctgatt ccggtgaacc tgaagaagaa agtgacctgg
    15781 gaagacggga aaagcgagcg cgtgctttac actcctgaaa tcgctgaaaa ccagcaaatg
    15841 atctcgctgt tatcaccgtt ccagaacaaa ggcaaagcgc agctggaagt gaaaataggc
    15901 gaaaccaatg gtcgtctgga aggcgatcgt gacaaagtgc gttttgtaca gactaatatg
    15961 gggcggttga ttctggcagc ccaaatggat cgcactggtg ccgactttgc ggtgatgagc
    16021 ggaggcggaa ttcgtgattc tatcgaagca ggcgatatca gctataaaaa cgtgctgaaa
    16081 gtgcagccat tcggcaatgt ggtggtgtat gccgacatga ccggtaaaga ggtgattgat
    16141 tacctgaccg ccgtcgcgca gatgaagcca gattcaggtg cctacccgca atttgccaac
    16201 gttagctttg tggcgaaaga cggcaaactg aacgacctta aaatcaaagg cgaaccggtc
    16261 gatccggcga aaacttaccg tatggcgaca ttaaacttca atgccaccgg cggtgatgga
    16321 tatccgcgcc ttgataacaa accgggctat gtgaataccg gctttattga tgccgaagtg
    16381 ctgaaagcgt atatccagaa aagctcgccg ctggatgtga gtgtttatga accgaaaggt
    16441 gaggtgagct ggcagtaatc cgaaagtgcc ggatgtttgc atccggcaca atgcttaatc
    16501 gcggcgggcg atatcagcaa atttggcatc gaggatcttt gccagatcgc ctgccgccag
    16561 ttcgatatcc agtccgcgct tgccgccgga aacataaata gtggcaaatt cttgtgcggg
    16621 ggcgtcgata atcgttggca gacgtttttt ctgccccagt gggctaatcc ccccaaccag
    16681 gtatcccgtc gaacgctgcg cgaccatcgg atcggccatc tcaactttct tggcacccag
    16741 cgcttttgct acttttttaa gatccagttg acctgcgacc ggcgtaacgg ccacggcaag
    16801 gtgtttcata tcaccgttca ctgccaccag cagcgttttg tagacctgat ccggattcaa
    16861 acctaatttt ttgacgactt catcgccaaa attggtttca gccggatcgt gctcgtaggt
    16921 atggatttga aacgaaatct tgtttttttc gagtaattta actgcgggtg tcatggtaat
    16981 tttcctgcct taaacaaaaa aacgaagcaa gcatacgcct gatggcggtc caaaaaaaag
    17041 agtcatcttg cctaagagta ttggcaggat ggtgagattg agcgacaatc gagttacacc
    17101 gtcgcttaaa gtgacggcat aataataaaa aaatgaaatt cctctttgac gggccaatag
    17161 cgatattggc cattttttta gcgcaacatt tgcggcaaat tcccttctcc atacaggtgt
    17221 agtgcaccga ccgcgaccac atatcgcccc ggcggcatgg cgcgtaattt atcccgccag
    17281 gcgagatttc gctgatgcat cagcacatcg tacagcgact gactgaacgt attgggcagc
    17341 gttatatcat tattttgcgg cggtgcattc agccaccagc tcatcatttg ttgcagcaac
    17401 cgtgcgttgg tatgccagtg ggtcagcgta tcgtccagca gcgccagtcc tttgtcaggg
    17461 agctggagca acatggcaat ctggttttca gccccttcca gttcaatcac gggtttatgt
    17521 tgttgcttcg ccgcctgcaa taactggtaa tcgataccgt attctgcccg cagccccagt
    17581 ttttgtgcct gcgtcgcctg aagaaccatc gcgatttgcc acagcggttg agtagaaaag
    17641 agcgacggag aaatgcccat ctcctggcta atgtgctgca gattttggag ttgctcctcg
    17701 ttaatgcgct cttccagcgc ctcgcaagta ggcaaattag caaaaggcgt atcgctggtg
    17761 gaaacatccg cctcgacgat cagcgcatcg gcgtttttga gctttttgag caaacgggtg
    17821 ggcaggggag ccatatcgtg gctacccata tgaatactgc caatcagatg aaaatggcga
    17881 ttgccgggaa gggtgatatc gatggcaggc caggtgtaat gattaccgcg cagcgcggcc
    17941 caaagcgttt ttacccggta caacagatcc atacgacctc cctttgtgaa atatcatgct
    18001 agcgcgcggt gagggatggc gcaaatggag ttggggactt gtttaatgtg tttgtatgat
    18061 tcagtatgtt cttgcatcgc tattcacagg gaagcaacag ttaaaaacca tgaaacaggc
    18121 aacaagaaaa ccgacgacac ccggagatat tcttctctat gaatatctgg aaccgctcga
    18181 tttgaaaatc aatgagttag cagagttgct gcatgttcat cgtaatagcg tcagtgctct
    18241 gatcaataac aatcgtaaac tcaccactga gatggcattc cgtctggcga aagtttttga
    18301 taccacagtc gattttaggc taaacctcca ggcggcagtt gatctttggg aagttgaaaa
    18361 caacatgcgc acccaggaag aattgggacg gattgaaaca gtggctgaat atttggcacg
    18421 ccgtgaagag cgtgcaaaaa aggtcgcgta aggccaaaac atcgcttcat ctcgtaggcc
    18481 ggataaggcg tttacgccgc atccggcagt catgcgtcga tgccaaatgt gccaccctaa
    18541 agcagcgcat ccgcaatgac atacttattc cttcggttta aaccgcagca accggttggc
    18601 gttactcact acggtaatcg acgagagcgc cattgccgct ccggcaacta ccgggttaag
    18661 cagtgttcca gtaaacggcc acaaaatacc ggcggcgacc ggaataccga tactgttgta
    18721 gataaaggca ccgagcaggt tctgcttcat gttgcgcagc gttgcgcggg aaatcgcgag
    18781 cgcatccgca acgcccatca ggctatggcg catcagggta atcgccgcgg tttcaatggc
    18841 aacatcactg ccgcccccca tcgcaatacc gacatccgcc tgggccagcg ctggcgcatc
    18901 gttaatgccg tcgcccacca ttgccacctg acgtccttca ctttgcaggc gtttaatcgc
    18961 ttcggcttta ccgtccggca gcaccccggc gatcacttca tcaatcccgg cttctttggc
    19021 gatcgcattg gcggtggttg ggttatcccc ggtcaacatc accagacgat atcccgcttt
    19081 atgcaggcgt tgcagcgccg ccacgctatc actacgcaat ggatcgcgta ctgccagcag
    19141 ggctaccgct ttcccgtcaa ccgccagcag cacaggcgtt gccccttgcg atgcctgagc
    19201 agtaatctcc gcttcgatag ctttggtacc aacctgttgc tcatttaaca gcgcctgatt
    19261 gcccagcaat aacgcatgac cttcagcttc accgctcacg cccagcccgc gcaatgtgcg
    19321 gaaaccgttg acctgcggca gctgcatatc acctgcttta tcgaggatcg cccgtgccag
    19381 cggatggctg gaaccttgct ccagtgccgc cgccagacgc aatgcctgcg cttcatcaac
    19441 atcagcaaac gttttcactg cgacaacctg cggcttccct tcggtcagcg tcccggtttt
    19501 atcgaacacg acggtatcga gtgtactggc gcgttgcagc gcgtcggcgt cccgcaccag
    19561 cacgccaaac tcagccgccc gcccgacgcc ggaaataatc gacatcggcg tcgccagccc
    19621 cagcgcacac ggacaggcga taatcagtac cgtggtggca atcaccaggg tataaacaat
    19681 ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg accagcgcaa tcaccaccac
    19741 cacaggtaca aatacggctg agattttatc cgccagctga ccgatttctg gcttgctgct
    19801 ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg gtatggctgc caaccgcact
    19861 ggcacgaaac agcacgctac cgtcctgtac cactgtcccg gcatggacgc tttcgccttc
    19921 gcctttttgc tgcgggattg gttcgcccgt cagcatcgct tcatccagcc atgcttcgcc
    19981 ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc gtcaggcgca gcaacatacc
    20041 tggctgcact tccgccagag gcacgctttt ttcaccttcg tcagtaacca ggcgcgccgt
    20101 cggtggggtt aaatcgagta atttctccag cgccttagaa gaacgctgac gtgcgcgtgc
    20161 ttccagcata tggccgagat tgatcagacc gataatcatc gcgctggctt cgtaataaag
    20221 atgtcgcgct tccatcggga accactgcgg ccacaggttg acgctcatcg aatagagcca
    20281 cgccacgcca gtacccagcg ccaccagcgt atccatcgtc gctgcaccgt tcagcaggct
    20341 tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc atcaccgcca gggttatcag
    20401 gccgataacc aaccacaggc tgcggttgtc agcggtgacc atcatgttat cgccaatcat
    20461 cccccagacc atcaccggga tacccaccgc cagtgcgaca attgcctgcc agcggaagcg
    20521 cttcatcgta gcaacggcgg tttcctgctg gcgctcacgg cgtttcgcgt catcttcaat
    20581 cgcttccgcg ccgtagcccg ctttttccac cgcctgcact aaatcttgtg gggaggcact
    20641 gcccatcacc agcgcagtac gctccgccag gtttacccgt gcctgagtga cgcccggtac
    20701 gctttgcagc gcattttgta cgcgggtgac acagctggcg cagctcatgc cgctcagcag
    20761 caactgctgg ctgtcatcgt catcggcggt cgctgccgga agcgcctcag aaaccgctgt
    20821 cagtgcttcc gacgggattg atgactccgc cagcggttta gcctttgggt ggcttacaga
    20881 tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt tctgcactgg cagtcccggt
    20941 aacgtgcgct tcagtgatag acacatccgc ttgttccaca tccgggcgct gttcgagact
    21001 ttctttcacg cgtttaacgc agtgaccgca ggacaggccg tccagggtca ggtcgatagt
    21061 ttgtgacata aaacactcct ttaagacagt tttgactggc tgtgataaag gttaaacctt
    21121 ccagcaaggg gaaggtcaag aaattaataa accaggcgag taaaagtccg taaagattaa
    21181 aaaattggct caatttgcat caggattaga catttatctc tttgttttcc tgtagttaag
    21241 ttgcgggtgc taagttaaat caggatgcct caaaatcggc accggggtga ggaattacct
    21301 cccgcatcta taaaaaggag ttaacaaaag atgttagatg caaacaaatt acagcaagca
    21361 gtggatcagg cttacaccca atttcactca cttaacggcg gtcaaaatgc cgactacatt
    21421 ccctttctgg cgaatgtgcc tggtcaactg gcggcagtgg ctatcgtgac ctgcgatggc
    21481 aaggtctata gtgcgggtga cagtgattac cgctttgcgc tggaatccat ctcgaaagtc
    21541 tgtacgttag cccttgcgtt agaggatgtc ggcccgcagg cggtacagga caaaattggc
    21601 gctgacccga ccggattgcc ctttaactca gttatcgcct tagagttgca tggcggcaaa
    21661 ccgctttcgc cactggtaaa tgctggcgcc attgccacca ccagcctgat taacgctgaa
    21721 aatgttgaac aacgctggca gcgaatttta catatccaac agcaactggc tggtgagcag
    21781 gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa ccaacttcca taaccgggcc
    21841 attgcctggc tgctgtactc cgccggatat ctctattgtg atgcaatgga agcctgtgac
    21901 gtgtacaccc gtcagtgctc cacgctcctc aatactgttg aactggcaac acttggcgcg
    21961 acgctggcgg cggggggagt gaatccgctg acgcataaac gcgttcttca ggccgataac
    22021 gtgccgtaca ttctggccga aatgatgatg gaagggctgt atggtcgctc cggtgactgg
    22081 gcatatcgcg ttggtttacc gggcaaaagc ggtgtaggtg gcggtattct ggcggtcgtc
    22141 cctggtgtga tgggaattgc cgcgttctca ccaccgctgg acgaagaagg caacagtgtt
    22201 cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg gctataacgt gtttaagggc
    22261 tgatcatgat gaacacggaa ggtaataacg gtaacaaacc cctcggtcta tggaatgtcg
    22321 tttccatcgg tattggggca atggtggggg cggggatctt cgcgctgctg gggcaggctg
    22381 cgttgctaat ggaagcctcg acctgggtcg cctttgcttt tggcggtatt gtggcgatgt
    22441 tttccggtta tgcctatgcg cgtctggggg cgagctatcc cagcaatggc ggcattatcg
    22501 acttctttcg tcgcggatta ggcaacggcg tcttttcgct ggcgctctcg ttactgtacc
    22561 tgttgacgct ggcggtgagc atcgccatgg tcgcccgtgc ttttggcgct tatgccgtgc
    22621 agtttttgca tgaaggcagc caggaggagc accttatttt gctctacgcg ttggggatca
    22681 ttgcggtgat gacgcttttc aactccttaa gcaaccatgc ggtagggcgg ctggaagtga
    22741 tcctcgtcgg cattaaaatg atgatcctgt tattgctgat tattgccggt gtctggtcgc
    22801 tgcaaccggc gcatatttcc gtctctgcgc cccccagctc cggtgcgttc ttctcctgta
    22861 tcgggataac cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg gcggataaag
    22921 tgaaagatcc gcaggtcatt atgccgcggg cgtttctggt ggcgattggc gttaccacgt
    22981 tgctttatat ctcgctggca ctggttttgc ttagcgatgt atcggcatta gagttagaaa
    23041 aatatgccga taccgccgta gcgcaggctg cttctccgct gctcggacat gtgggttatg
    23101 tgatcgtcgt catcggcgct ttactggcga cggcttcagc cattaacgcg aacctgttcg
    23161 ccgtgtttaa catcatggac aacatgggca gcgaacgcga actgccgaag ctaatgaata
    23221 aatccctgtg gcggcagagt acctggggca acatcattgt cgtggtgttg attatgctga
    23281 tgacggcggc gctaaattta ggctcactcg ccagtgttgc cagcgccacc tttttgattt
    23341 gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac gcctcgttgc
    23401 cgattcttat cgttggtacg ttggtgatgt tgttggtgat cgttggcttt atctacagtc
    23461 tgtggtccca gggtagccgt gcgttgatat ggattattgg ctcactctta ctcagcctta
    23521 ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca tctctctgtg cgcagtactt
    23581 cctgtattat tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc ttgaatatct
    23641 gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat cagcgatgta
    23701 gcaaaaatta ccggcctgac cagcaaagcc attcgcttct atgaagagaa ggggctggtg
    23761 acgccgccga tgcgcagcga aaacggttat cgcacctaca cgcagcagca tctcaacgaa
    23821 ctgaccttac tgcgccaggc acggcaggtg ggctttaacc tggaagagag cggcgagctg
    23881 gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg tcaaacggcg cacgctggag
    23941 aaggtggcgg agatcgaacg acacattgag gagctgcaat ccatgcgcga ccagctgctg
    24001 gcactggcga atgcctgccc tggcgatgac agcgccgact gcccgattat cgaaaatctc
    24061 tccggctgct gtcatcatcg ggcagggtga ttaagacgag acggctcgga tatgtagggt
    24121 tatcccttct atcgcaatga cttcaacatg cgtacctgca ccgagatcct cgctggcgct
    24181 gacaggccat gaactgtcac cgacgcgcat atgaccgcgc ccgttgacca gcggagattc
    24241 cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg tttaaatgac tatcgctgtg
    24301 cttttgttcg cgcacccgcc gcgacaacca tttccaccac agccaggcgg cgagcagcgt
    24361 caggatggca aacattaccc cttgccactc ccaacccagc ggcaccagcc agaccaccag
    24421 gccggtaatc accgctgcca cgccgctcca caacaaataa ccatttccgc ccagcatctc
    24481 ggctgccagc agcaaaccgc cgagactgag ccagaaaata tgtggatgaa cgaccattaa
    24541 ctccatcatg gctgagtccg tttgttggcg ctgtctttca ccagctcggc aatcccggca
    24601 atcgacccca tcaggctgct ggcctctaat ggcatcatca ctactttgct gttactggag
    24661 gaaccgatct gctgtaacgc ttcggtgtat ttctgcgcta cgaagtagtt caccgcctga
    24721 atatcaccgg aggcgatagc ttcagacacc attttggtgg cgcgggcttc tgcttcggcg
    24781 gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac gttcgccttc cgctttcagg
    24841 atttgcgact gtttttcacc ttcggctttg aggatttccg cctgacggat cccttccgct
    24901 tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct gcgcgttcat tgaagagata
    24961 agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg tgactttaat cccccacggg
    25021 ttggtggctt catcgacaat acgcagcagg cgtgagttga tgctgtcgcg ctgagagagc
    25081 atttcgtcaa gttccattga acccaacacg gtacggatgt tagtcatggt caggttgatg
    25141 atcgccagct ccagattgct gacttcataa gccgcgcgcg gcgcgtcaat cacctgaata
    25201 aaacagactg cgtcgatggt aacgttggcg ttatctttcg agataacttc ctgggaaggg
    25261 atatcgagca cttgctccat catattgatc ttgcgaccaa tgcgatccat aaacggcacc
    25321 accagactga gccccggctg taacgttttg gtatagcgac caaaacgttc taccgtccat
    25381 tgatagccct gcggcacgat tttgacgccc gcgccgacaa tgaccagcgc gacaaaaatg
    25441 agaatcggga taaagataag catcggaaaa acctcctgtt gtaccgtcca taatcagcaa
    25501 aattgctgct tgattaaaca aattatacct gattactgaa agagagttcc cccttattcc
    25561 tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg gaacagtgat gcaggaaaat
    25621 agtcctttgc ttcagctaca aaacgtagga tatctggcgg gtgatgcgaa gattcttaat
    25681 aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa ttaccggtcc ttccggttgt
    25741 ggcaaaagta cgctgctaaa aatagttgct tcattgatca gcccaaccag cggaacgtta
    25801 ctgtttgaag gtgaggatgt cagcacacta aagccagaaa tctaccgcca gcaagtctct
    25861 tactgcgccc agacgccgac gctgtttggc gatacggtat acgataatct gatctttccc
    25921 tggcagatcc gtaaccagca gcctgacccg gccatttttc tcgattttct cgaacgcttc
    25981 gctttgccgg acagcatttt gacgaaaaat atcgccgagc tatctggtgg tgaaaaacaa
    26041 cgcatctcac tgattcgtaa cctgcaattt atgccgaagg ttttattgct ggatgaaatc
    26101 accagtgcgc tggatgaaag taataaacat aacgttaatg agatgatcca tcgttatgtg
    26161 cgcgagcaaa atattgccgt gctgtgggtg acacacgata aagacgaaat taatcatgcg
    26221 gataaagtga ttacactgca accgcatgcc ggagaaatgc aggaagcacg ctatgaactc
    26281 gcataatatt actaacgaat cattggcact ggcattaatg ctggtggtgg tggcaatctt
    26341 aattagccat aaagaaaaac tggcgctgga gaaagatatt ttctggagcg tcgggcgagc
    26401 gataattcag ctgattattg tcggctatgt gctgaagtat attttcagcg tggatgatgc
    26461 cagcctgaca ttattgatgg tgttatttat ctgctttaat gcggcgtgga acgcgcaaaa
    26521 acgcagtaaa tatattgcta aagcttttat ctcatcgttt attgccatta cggtcggggc
    26581 gggaattacc ctggcggtgc tgattctctc tgggtcgatt gaatttatcc cgatgcaggt
    26641 gatccctatc gccgggatga ttgccggtaa cgccatggta gcggtggggt tgtgttacaa
    26701 caatttaggg caacgggtca ttagcgaaca gcaacagatt caggagaaac tgagtcttgg
    26761 tgcgacgccg aagcaggctt cagcgatatt gattcgcgac agtattcgcg cggctttaat
    26821 tccgacggtc gattcagcaa aaacggttgg cttagtgagt ttaccaggaa tgatgtccgg
    26881 gctgatattt gccgggattg atccggtgaa ggcgattaaa tatcagatta tggtgacctt
    26941 tatgctgctc tcaaccgcca gcttgtcgac cattattgcc tgctatttaa cctatcgtaa
    27001 gttttataat tcgcgccacc agttggtggt gacgcaattg aagaagaaat gatgcgatgc
    27061 cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc cgatcaacgg aatatcaata
    27121 caacaatgca tacagctggc ggcgatactt cgacgccagt gcatcacccg tacccagcgc
    27181 agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct gcggcggtga gatctttacg
    27241 cagatgcccg aacagcaact ccagcgcctc ttcattgcgc ccaacctggt gcagttgcag
    27301 agccagttgc gtcgccagtg cggcatcttc tggattctcc gccacctgct gttgcaactg
    27361 ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg atttgcgcca ccagcccctg
    27421 gtagcgggtg tcctgatcct gcaacggaat ggttttcagc accgcttccg catcttcaga
    27481 acggttcagc gcaatcagcg tttctgccag cagcaggccg atctccccgt tctgattcga
    27541 caactgccag gcgtctttca gcaatggcag ggcatcggtg taattgcctt cctgcatcag
    27601 ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc agcactttat ccagcagggc
    27661 gcggatcgcc tcttccggtt gcggcccctg gaagccatct accggttgcc cgttctggaa
    27721 cagatacacg gtcggaatcg cgcgcagacc aaactgggcg gcaatcatct gctccgcgtc
    27781 acagtccagc ttcgccagaa taaattgccc gttgtactgc gccgcgaggc tttccaggat
    27841 cggggttaac tgcaaacagt gctggctacg ttcagaccaa aaatagaaca gcaccggagt
    27901 ggtcatcgac tgttcaagaa cctgttgcag gttagattcg ttaatgttga caatattttc
    27961 tacggacatg gagtcgctct ctgttgtcga tattttcttt gacatggggg cttaagcgcg
    28021 cgcttcaact cacccctgca atattttgtc catcacgcgc cccggcagca ggcgcttgag
    28081 caccattact gcccaggtca ccagcgttac cggatagcgc atcttcggct tctcgctaat
    28141 aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc aacgtaaagc gggcggcgat
    28201 gccgggattt tcgactggtt tatcactttg cgtctggttg acgttgtcgg tgaagcgagt
    28261 acgaatggga ccgggttcga tcaggctgac tttaattccg ctgtggcgca gctccatgcg
    28321 cagtgcgtct gaccacgcct ccagcgcata tttactggcc gcgtaagcgc cacgacccgg
    28381 cgtggagatt aatcccatca ccgatgatgt catcacaata cgcccttcac cgtgcggtaa
    28441 catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg aaaaagttgg cggaaaactg
    28501 ctgttccatc tgcgcacggc tgatggtgga aagggggcca tacatgccga atccggcatt
    28561 gttaaagatc ccatacagac aattatcggt cagggcgatc acctcgtctg ctgcgcgatc
    28621 aacactttct ggtgaatcca gatcgatcaa cacgccggta aatcccatgt tgttcatacg
    28681 ctcaacatca tccggttttc ggcaacctgc cagcacatga aaaccctggc gttttaattc
    28741 gagcgcgctt tccaggccaa ttccactgga acatccggta attaagaccg atttttgcat
    28801 aactttacct gtcaggatct ccgttgcttt atgagtcatg atttactaaa ggctgcaact
    28861 gcttcgccat ccagtcggca ataaacggct gggcgtcgcg gttgggatga ataccgtcat
    28921 cctgcatcca ttgtggcttg aggtagacct cttccataaa aaagggcagc agcggaacat
    28981 caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc ttcattataa cggcgaccat
    29041 agtttgcagg cagacgtatt tgcattaaca atggttcagc gttggcggct ttgacatcct
    29101 gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg aaaaccacgc aaaccgtcat
    29161 tgccgcccag ttcaaccagc acccaacgcg gctgatgctg tttcagcaga gccggaaggc
    29221 gtgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc attaactacc gacgttttac
    29281 tctgccactt atcattcaac aaggcaggcc aggccgcgct ggcagacatt cgatacccgg
    29341 cgctcaggct atcacccaga atcaataacg tgtccgctgc ggcggcacgg aaggttaaca
    29401 ggaccaggaa caggaagggc aaatgccagc ggaaaacatt gttgaagttc atcatcttaa
    29461 gaagtccgtc ggtcaggggg agcatgaact ctccatcctc accggagttg agctggttgt
    29521 caaacgtggc gagaccatcg cactggtggg cgagtcggga tcgggtaagt caaccttgct
    29581 ggcgatcctc gccgggcttg atgacggcag cagtggcgaa gtgagtctgg tgggacaacc
    29641 gctacataat atggacgaag aagcgcgggc aaagttgcgc gcgaagcacg tcggctttgt
    29701 ttttcagtca tttatgttaa ttcctaccct taacgcgctg gaaaacgtcg agcttccggc
    29761 tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg gcgaaagcgt tgctcgaaca
    29821 gttagggctg ggtaaacgtc tggatcatct tccggcacag ctttccggcg gtgaacagca
    29881 acgagtggcg ctggcacgag cctttaatgg tcgacctgat gtgctgtttg ccgacgaacc
    29941 caccggcaac cttgaccgcc agacgggcga taaaattgcc gacctgctgt tttccctcaa
    30001 ccgtgaacat ggcaccacgt tgattatggt gacccacgac ctgcaactgg cggcacgctg
    30061 cgaccgctgc ttacggctgg tgaacgggca gttgcaggag gaagcatgat tgcacgctgg
    30121 ttctggcgcg aatggcgctc gccttcgcta ttgattgtct ggctggcgtt aagcctggcg
    30181 gtggcctgcg tgctggcgct gggtaatatc agcgatcgca tggaaaaagg cttaagccag
    30241 caaagccgtg aatttatggc gggcgatcgg gcgttgcgca gttcgcgcga agtgccgcaa
    30301 gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg gcaagcagct gactttcgcc
    30361 accatgacat ttgcaggcga cacaccgcag ctggcgaacg ttaaagcggt ggatgatatc
    30421 tacccgatgt atggcgatct gcaaaccaat ccccctggcc tgaaaccgca ggcgggcagc
    30481 gtattgctgg ccccacgcct gatggcactg cttaacctga aaacgggcga caccattgac
    30541 gtgggcgatg ccaccttgcg gattgccgga gaagtgattc aggaaccgga ttccggtttt
    30601 aaccccttcc agatggctcc gcgtctgatg atgaatctgg cggatgtcga taaaaccgga
    30661 gccgtgcagc cggggagtcg ggtcacctgg cgctataaat tcggcggcag tgagaaccag
    30721 ctcgacagct atgagaaatg gttgttacct cagcttaaac ccgaacaacg ctggtacggt
    30781 ctggaacagg acgaaggcgc gctggggcga tcgatggaac gctcgcaaca gttcctgctg
    30841 ctttcggcgc ttctgacctt gctgctggca gtggcagcag tggcggtagc gatgaatcat
    30901 tactgtcgca gtcgctacga tctggtggcg atcctcaaaa cgctgggggc agggcgagcg
    30961 caactgcgta agctaatcgt cggtcagtgg ttgatggtgc tgacgctttc agccgttacc
    31021 ggtggggcga taggcctgtt gttcgaaaac gtgttgatgg tgctgctcaa gcccgttctg
    31081 cctgctgcac taccgccagc cagcctctgg ccgtggctat gggcgctggg caccatgacg
    31141 gtcatctcgc tgctggtggg gctacgacca taccgcttgt tgctggcaac gcagccttta
    31201 cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc tgaagtttta tctgccgatt
    31261 gtcagtgtag tggttgtgct gctgctcgcc ggattaatgg ggggcagtat gctgctctgg
    31321 gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg gtgtgctggg ctggatgctg
    31381 ctgaatgtac ttcgccgcat gacgctgaaa tcgctgcctc tgcgcctggc ggtcagccgc
    31441 ctgttacgtc agccgtggtc aacgttaagc cagctttcgg cattttcgct ctcctttatg
    31501 ctgctggcac tgctgctggt gttgcgtggt gatctgctcg accgctggca gcagcagcta
    31561 cctccagaaa gcccgaacta ctttttaatt aacatcgcca cagaacaggt agcaccgcta
    31621 aaagcgttcc tcgcggaaca tcagatagtc ccggaatcgt tttatccggt ggtgcgggcg
    31681 cggctgacgg cgattaacga taagccgaca gaaggtaatg aagatgaggc gcttaaccgc
    31741 gaactcaatc ttacctggca aaatacgcgg cccgatcata atccgattgt cgccggtaac
    31801 tggccgccaa aagccgatga agtgtcgatg gaagaggggc tggcgaaacg cttaaaggtt
    31861 gccctcggtg ataccgtgac ttttatgggc gatacccagg agttccgcgc taaagtgacc
    31921 agcctgcgca aagtggactg ggaaagtctg cggcctaatt tctattttat tttcccggaa
    31981 ggggcattag acgggcaacc gcagagctgg cttaccagtt tccgctggga gaatggcaac
    32041 ggcatgttga cgcaactcaa ccgccagttc ccgaccatta gcctgttaga cattggcgcg
    32101 attttaaaac aggtcggtca ggtgctggag caggtaagtc gggcgctgga ggtgatggtg
    32161 gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac aggtgcaggt gggtatgcgt
    32221 cagcgccacc aggagctggt ggtgtggcgc acactcggtg cggggaaaaa actgctgcgt
    32281 accacgttgt ggtgtgagtt cgccatgctt gggtttgttt ccggcctggt ggccgcaatt
    32341 ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt ttgattttcc gtgggagcca
    32401 gactggcgat tgtggattgt tctgccgtgc agcggagcgc tgctgctgtc gcttttcggc
    32461 ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt tcaggcagtt tgcggggtga
    32521 tgagagtaaa taaggggcgt tctggttgaa tggaacgcct tgtttaaatt aattctacaa
    32581 tatgtttcat tgatgatgtt attgaattgg tgatttcc
//
LOCUS       DAPGWD010000037        31756 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPGWD010000037 DAPGWD010000000
VERSION     DAPGWD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.037"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(204..1577)
                     /gene="argH"
                     /locus_tag="RHX88_001702"
     CDS             complement(204..1577)
                     /gene="argH"
                     /locus_tag="RHX88_001702"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDV2230398.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1638..2414)
                     /gene="argB"
                     /locus_tag="RHX88_001703"
     CDS             complement(1638..2414)
                     /gene="argB"
                     /locus_tag="RHX88_001703"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDV2230399.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2422..3426)
                     /gene="argC"
                     /locus_tag="RHX88_001704"
     CDS             complement(2422..3426)
                     /gene="argC"
                     /locus_tag="RHX88_001704"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDV2230400.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3580..4731
                     /gene="argE"
                     /locus_tag="RHX88_001705"
     CDS             3580..4731
                     /gene="argE"
                     /locus_tag="RHX88_001705"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDV2230401.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5083..7734
                     /gene="ppc"
                     /locus_tag="RHX88_001706"
     CDS             5083..7734
                     /gene="ppc"
                     /locus_tag="RHX88_001706"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDV2230402.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7917..9650
                     /locus_tag="RHX88_001707"
     CDS             7917..9650
                     /locus_tag="RHX88_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDV2230403.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9799..10650
                     /locus_tag="RHX88_001708"
     CDS             9799..10650
                     /locus_tag="RHX88_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2230404.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10637..10978)
                     /locus_tag="RHX88_001709"
     CDS             complement(10637..10978)
                     /locus_tag="RHX88_001709"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV2230405.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10980..11858)
                     /locus_tag="RHX88_001710"
     CDS             complement(10980..11858)
                     /locus_tag="RHX88_001710"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDV2230406.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVSAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(11824..14121)
                     /locus_tag="RHX88_001711"
     CDS             complement(11824..14121)
                     /locus_tag="RHX88_001711"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV2230407.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLYEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14172..14492)
                     /locus_tag="RHX88_001712"
     CDS             complement(14172..14492)
                     /locus_tag="RHX88_001712"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV2230408.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14507..15586)
                     /locus_tag="RHX88_001713"
     CDS             complement(14507..15586)
                     /locus_tag="RHX88_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDV2230409.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15895..18396
                     /gene="ptsP"
                     /locus_tag="RHX88_001714"
     CDS             15895..18396
                     /gene="ptsP"
                     /locus_tag="RHX88_001714"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV2230410.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18408..19070
                     /gene="fsa"
                     /locus_tag="RHX88_001715"
     CDS             18408..19070
                     /gene="fsa"
                     /locus_tag="RHX88_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV2230411.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19081..20184
                     /gene="gldA"
                     /locus_tag="RHX88_001716"
     CDS             19081..20184
                     /gene="gldA"
                     /locus_tag="RHX88_001716"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDV2230412.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QTTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            20459..21076
                     /locus_tag="RHX88_001717"
     CDS             20459..21076
                     /locus_tag="RHX88_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDV2230413.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21103..22008)
                     /gene="yijE"
                     /locus_tag="RHX88_001718"
     CDS             complement(21103..22008)
                     /gene="yijE"
                     /locus_tag="RHX88_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDV2230414.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22102..24282)
                     /gene="katG"
                     /locus_tag="RHX88_001719"
     CDS             complement(22102..24282)
                     /gene="katG"
                     /locus_tag="RHX88_001719"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDV2230415.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24611..25501)
                     /gene="metF"
                     /locus_tag="RHX88_001720"
     CDS             complement(24611..25501)
                     /gene="metF"
                     /locus_tag="RHX88_001720"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDV2230416.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25850..28282)
                     /gene="metL"
                     /locus_tag="RHX88_001721"
     CDS             complement(25850..28282)
                     /gene="metL"
                     /locus_tag="RHX88_001721"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDV2230417.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28285..29445)
                     /gene="metB"
                     /locus_tag="RHX88_001722"
     CDS             complement(28285..29445)
                     /gene="metB"
                     /locus_tag="RHX88_001722"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDV2230418.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29722..30039
                     /gene="metJ"
                     /locus_tag="RHX88_001723"
     CDS             29722..30039
                     /gene="metJ"
                     /locus_tag="RHX88_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDV2230419.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30223..30831
                     /locus_tag="RHX88_001724"
     CDS             30223..30831
                     /locus_tag="RHX88_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDV2230420.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            30976..31651
                     /locus_tag="RHX88_001725"
                     /pseudo
     CDS             30976..31651
                     /locus_tag="RHX88_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         7647 a         8855 c         8074 g         7180 t
ORIGIN      
        1 ttatggggag tgtgaaggta aagaagacac aatgataaaa aggaatgtga caaataatgg
       61 ttctggttgg gcgttaaatt taagctcacc agaatgctga caaatttctg cctgatgtgg
      121 cgtaaacgcc ttatccgcct acgaagtgtc gcactttgta ggcgtggtaa gctccccatt
      181 ttcaggggag caatataaac aaattactct aaccgcgcct gcgcaaaagc aattgcctgc
      241 gccacctgct gcggtgagac gccgcctttt gccgcacgct tgtcgaggca cgattgcagc
      301 gacagaatcg gatagacatc ttcgtcaatc accgggctga atttctgcaa ctcgtcgagc
      361 ggcagatctt ccagcggttt gccctgacga atggcttcca ccaccgcttc accaacaata
      421 tggtgcgcct cgcggaacgg tacgcctttc gccaccagat aatccgccag ttcggtggcg
      481 ttggcgtaac cctgctgagc cgcttcctgg caacgtggac gtttcacctg aatgccgtcc
      541 agcaccagcg ccgccatatg caggcagtcc agccaggtat cgagcgcgtc gaacagacct
      601 tctttgtctt cctgcatatc tttgttgtaa gccagcggca aacctttcag cgtcatcatc
      661 atgccggtta acgccccctg cacccgaccg catttaccgc gaatcagctc cagcgcatcc
      721 gggtttttct tctgcggcat taatgatgaa ccggaagtca cgcggtcaga aagctccaca
      781 aaccccgctt cgccggtgtt aaagaaaatc agatcttcag caaaacgcga cagatgcacc
      841 atgccgatag cggcagccga cagcagttcc aacacatggt cacggtcaga aacgctgtcc
      901 agactgttac gggtcgccga agcaaagccc agccagcctg ctaactgttc acggtcgatt
      961 tcataggccg ttcccgccag cgcgccacag cctaatgggc tgacatccag acgcttaagc
     1021 gcatcctgca agcggctttc atcacgcgcc agcatctcaa cataggccag gcaccagtgc
     1081 gcgaacgtca ccggctgggc gcgttgcagg tgagtgtaac ctggcatcac cgcgtcctga
     1141 ttgttttgtg cggtttccac cagcgccgat tgcagctgcc ggttagccgt cagcaactcg
     1201 ccaacggtat ctttgcacca cagtttcagg tctgtcgcta cctgatcatt acggctacgc
     1261 ccggtatgca gctttttgcc taactggccc actttgtcga tcagtttgcc ttccacccag
     1321 ctatggatat cttcggcgtc gctttcaagg atttgttgtg gcctggcgcg aacatcttcc
     1381 agcaacacgt tcagcgcctc ttccagttgc gcctgctctt ctgcggttaa cacgccgacc
     1441 gttaccaggg ctttggacca ggccacagag ccaacaatat cctgctccgc cagacggtaa
     1501 tcaaagcgca gtgagtcgtt gaattgtttg aaccgttgat ctgctgcctg ggtaaaacgc
     1561 ccgccccaaa gtgccataac tctgtttcct tattttgaaa ttcaatgccg gaaagctgcg
     1621 cctccggcca acaaaactta agctaaaatc cgcgtaccca tcggcatacc gttaaacagt
     1681 gccggaagct gctccgcatg acgccaggag gcgatatcta ccggacggcc cagcgtgcgg
     1741 gccgcatcca gcgccgcgtt cactttcact atcatgccgt cagtaataat gccctgctca
     1801 atcagttgtt ctgctttcgc ggcggtcatt tcggcaatgc gttgcccttt gccgtcgaga
     1861 atgccgctga cgtcggagag caaaatcaga tccgcgccca gcgttgccgc cagcgccgtt
     1921 gccgcctggt cggcattgac gttcatcagt tgcccttcgt ctgttacgcc aatggagctg
     1981 accaccggca gataaccgtt ctccagcaag gagttgataa gcttaggcga acctggctgc
     2041 gccagtccaa catgacctaa ctcttcatca agctgggtca ctttgacgct gtcgccgtca
     2101 ccgagaaaca aacctacggc cgcaatctga tgtttcttcg cccatgccaa cagggtttta
     2161 tttgccgttc ccgccagtgc tccggtgata atgtctatct gatcagcagg cgtcacccgc
     2221 aggccgtttt tctttttcac cggcagattc agccctttca tcagctcatc caccacgcaa
     2281 ccgccgccgt gcacaatcac cagcggacgc tgatgtgact cacgataatt caccagtgcg
     2341 ctaaacagac gttccagcgc ctcttcacta tccagcagta cgccgcccag tttgataatt
     2401 aatggattca tcattgcacc cttaaataag agactgcgtt tccgcatagc cgaaacgaat
     2461 attggcgcac tgtaccgctt gtgccgccgc gcctttcagt aagttgtctt cggtcgccac
     2521 aataatcaga tgctcgccct gaacggcaaa cccgatatcg caaaatggca gcccaacgac
     2581 atttttcagc gccggaacgc ctttgtcata cagccgcacc agcggtttat gggcatacgc
     2641 ctgctgtaac acttgcgcga cttgcgcctg ggtcacaccc gatttcaggc ggcaggtaat
     2701 ggtttcgaga atgccgcgcg ggaaattgcc cagatgtggg gtgaagataa cgtcagcacc
     2761 gaggtgtgtg gcgatctctg gttgatggcg atgagtaaag acgccatacg gttgcaggct
     2821 aacttcacaa aagctgtttg aaatggccgc tttacgccct gcaccgctca cgccgctggt
     2881 ggcgttgatc accggccact gattgaggtc aagaagatcg gcatcaatca acggtttcag
     2941 cgccagctgt gccgccgtcg gataacagcc cggcaccgca atcaaattcg cttcttttaa
     3001 tttattaccg caccactccg ccagaccgta ggctgcctgt tccaacagtt ccgggtattg
     3061 atgggtaaag ccgtaatatt tttcatagaa ggtggcgtcg ttaacacgaa acgcgccgga
     3121 aaggtcgaac accacgcagc ccgcttcaag aaactgcggc gctaaatcgt ggctaacttc
     3181 atgggcggtg gcgagaaaca ctacgtccac ccctgggcta aactcgctga tatccgacat
     3241 cggctgcaac ggcagatcaa cgatgccttt tagctgcgga tgcaaatcgg agattaactt
     3301 tcccgcatca ttgctttgcg ctgaaacagt caaagcggtt atgttcatat gcggatggcg
     3361 atttacatag gtcactagct ctgcgccagc gtagccgctg gcacccacaa tcagcgtatt
     3421 caacatcggg gctattcacc ttcttatgtc tggttgccag gttaaacgta aaacattcac
     3481 cttacggctg gtgggtttta ttacgctcaa cgttagtgta tttttattca taaatactgc
     3541 atgaatattg atactatcat gaccagaggt gtgtcaacaa tgaaaaacaa attaccgcca
     3601 tttatcgaga tttaccgcgc tctgattgcc acaccttcaa taagcgccac ggaagaggca
     3661 ctcgatcaaa gcaatgcaga tttaatcact ctgctggcgg actggtttaa agatttgggc
     3721 ttcaatgtgg aagtgcagcc tgttccagga actcgcaaca aatttaatat gctggcaagt
     3781 atcggacagg gggctggcgg cttgttgctg gcggggcata ccgatacggt gccatttgat
     3841 gacggtcgct ggacgcgcga tccgtttaca ctgacggagc atgacggcaa gctttacggc
     3901 ttaggcaccg ccgacatgaa aggctttttt gcgtttatcc ttgatgcgct acgcgatgtc
     3961 gacgtcacga aactgaaaaa accgctctac attctggcga ctgctgatga agaaaccagt
     4021 atggccggag cgcgttattt tgccgaaact accgccctgc gcccggattg cgccatcatt
     4081 ggcgaaccga cgtcactaca accggtacgc gcacataaag gtcatatctc taacgccatc
     4141 cgtattcagg gccagtcggg gcactccagc gatccagcac gcggagttaa cgctatcgaa
     4201 ctaatgcacg acgccatcgg gcatattttg caattgcgcg ataacctgaa agaacgttat
     4261 cactacgaag cgtttaccgt gccataccct acgctcaacc tcgggcatat tcacggtggc
     4321 gacgcttcta accgtatttg cgcttgctgt gagttgcata tggatattcg tccgctgcct
     4381 ggcatgacac tcaatgaact taatggtttg ctcaacgatg cattggctcc ggtgagcgaa
     4441 cgctggccgg gtcgtctgac ggtcgacgag ctgcatccgc cgatccctgg ctatgaatgc
     4501 ccaccgaatc atcaactggt tgaagtagtt gagaaattgc tcggagcaaa gaccgaagtg
     4561 gtgaactact gtaccgaagc gccgtttatt caaacgttat gcccgacgct ggtgttgggg
     4621 cctggctcaa ttaatcaggc tcatcaacct gatgaatatc tggaaacacg gtttatcaag
     4681 cccacccgcg aactgataac ccaggtaatt caccattttt gctggcatta aaacgtaggc
     4741 cggataaggc gctcgcgccg catccggcac tgttgtcaaa ctccagtgcc gcaataatgt
     4801 cggatgcgat acttgcgcat cttatccgac ctacaccttt ggtgttactt ggggcgattt
     4861 tttaacattt ccataagtta cgcttattta aagcgtcgtg aatttaatga cgtaaattcc
     4921 tgctatttat tcgtttgctg aagcgatttc gcagcatttg acgtcaccgc ttttacgtgg
     4981 ctttataaaa gacgacgaaa agcaaagccc gagcatattc gcgccaatgc gacgtgaagg
     5041 atacagggct atcaaacgat aagatggggt gtctggggta atatgaacga acaatattcc
     5101 gcattgcgta gtaatgtcag tatgctcggc aaagtgctgg gagaaaccat caaggatgcg
     5161 ttgggagaac acattcttga acgcgtagaa actatccgta agttgtccaa atcttcacgc
     5221 gctggcaatg atgctaaccg ccaggagtta ctcaccacct tacaaaattt gtcgaacgac
     5281 gagctgctgc ccgttgcgcg tgcgtttagt cagttcctga acctggccaa caccgccgag
     5341 caataccaca gcatttcgcc gaaaggcgaa gctgccagca acccggaagt gatcgcccgc
     5401 accctgcgta aactgaaaaa ccagccggaa ctgagcgaag acaccatcaa aaaagcagtg
     5461 gaatcgctgt cgctggaact ggtcctcacg gctcacccta ccgaaattac ccgtcgtaca
     5521 ctgatccaca aaatggtgga agtgaacgcc tgcttaaaac agctcgataa caaagatatc
     5581 gctgactacg aacacaacca gctgatgcgt cgcctgcgcc agttaatcgc ccagtcatgg
     5641 cataccgatg aaatccgtaa gctgcgtcca agcccggtag atgaagccaa atggggcttt
     5701 gccgtagtag aaaacagcct gtggcaaggc gtaccgaatt acctgcgcga actgaacgaa
     5761 caactggaag agaacctcgg ctacaaactg cccgtcgaat ttgttccggt acgttttacc
     5821 tcgtggatgg gcggtgaccg cgacggcaac ccaaacgtca ctgccgatat cacccgccac
     5881 gtcctgctgc tcagccgttg gaaagccacc gatctgttcc tgaaagatat tcaggtgctg
     5941 gtttctgaac tgtcgatggt tgaagcgacc cctgaactgc tggcgctggt tggcgaagaa
     6001 ggtgccgcag aaccgtatcg ctatctgatg aaaaacctgc gttctcgcct gatggcgaca
     6061 caggcatggc tggaagcgcg cctgaaaggc gaagaactgc caaaaccaga agggctgctg
     6121 acacaaaacg aagaactgtg ggaaccgctc tacgcttgct accagtcact tcaggcgtgt
     6181 ggcatgggta ttatcgccaa cggcgatctg ctcgacaccc tgcgccgcgt gaaatgtttc
     6241 ggcgtaccgc tggtccgtat tgatatccgt caggagagca cccgccatac cgaagcgctg
     6301 ggcgagctga cccgctacct cggtatcggc gactacgaaa gctggtcaga ggccgacaaa
     6361 caggcgttcc tgatccgcga actgaactcc aaacgtccgc ttctgccgcg caactggcaa
     6421 ccaagcgccg aaacgcgcga agtgctcgat acctgccagg tgattgccga agcgccgcaa
     6481 ggctccattg ccgcctacgt gatctcgatg gcgaaaacgc cgtccgacgt actggctgtc
     6541 cacctgctgc tgaaagaagc gggtatcggg tttgcgatgc cggttgctcc gctgtttgaa
     6601 accctcgatg atctgaacaa cgccaacgat gtcatgaccc agctgctcaa tattgactgg
     6661 tatcgtggcc tgattcaggg caaacagatg gtgatgattg gctattccga ctcagcaaaa
     6721 gatgcgggag tgatggcagc ttcctgggcg caatatcagg cacaggatgc attaatcaaa
     6781 acctgcgaaa aagcgggtat tgagctgacg ttgttccacg gtcgcggcgg ttccattggt
     6841 cgcggcggcg cacctgctca tgcggcgctg ctgtcacaac cgccaggaag cctgaaaggt
     6901 ggcctgcgcg tgactgaaca gggcgagatg atccgcttta aatatggtct gccagaaatc
     6961 accgtcagca gcctgtcgct gtacaccggg gcgattctgg aagccaacct gctgccacca
     7021 cctgagccga aagagagctg gcgtcgcatt atggatgaac tgtcagtcat ctcctgcgat
     7081 ctctaccgtg gctacgtacg tgaaaacaaa gattttgtgc cttacttccg ctccgctacg
     7141 ccggaacaag aattgggcaa actgccgttg ggttcacgtc cggcgaaacg tcgcccaacc
     7201 ggcggcgtcg agtcgctgcg cgccataccg tggatttttg cctggacgca aaaccgcctg
     7261 atgctccccg cctggctggg tgcaggtacg gcgctgcaaa aagtggtcga agatggcaaa
     7321 cagagcgagc tggaagccat gtgccgcgat tggccattct tctcgacgcg tctcggcatg
     7381 ctggagatgg tcttcgccaa agcagacctg tggctggcgg aatactatga ccaacgcctg
     7441 gtagacaaag cactgtggcc gttaggtaaa gagttacgca acctgcaaga agaagacatc
     7501 aaagtggtgc tggcgattgc caacgattcc catctaatgg ccgatctgcc gtggattgca
     7561 gagtctattc agctacggaa tatttacacc gacccgctga acgtattgca ggccgagtta
     7621 ctgcaccgct cccgccaggc agaaaaagaa ggccaggaac cggatcctcg cgtcgaacaa
     7681 gcgttaatgg tcactattgc cgggattgcg gcaggtatgc gtaataccgg ctaatcttcc
     7741 tcttctgcaa accctcgtgc ttttgcgcga gggttttctg aaatacttct gttctaacac
     7801 cctcgttttc aatatatttc tgtctgcatt ttattcaaat tctgaatata ccttcagata
     7861 tccttaagga attgtcgtta cattcggcga tatttttttc aagacaggtt cttactatgc
     7921 attccacaga agtccaggct aaaccccttt ttagctggaa agccctgggt tgggcactgc
     7981 tctacttttg gtttttctct actctgctac aggccattat ttacatcagt ggttatagtg
     8041 gcactaacgg cattcgcgac tcgctgttat tcagttcgct gtggttgatc ccggtattcc
     8101 tctttccgaa gcggattaaa attattgccg cagtaatcgg cgtggtgcta tgggcggcct
     8161 ctctggcggc gctgtgctac tacgtcatct acggtcagga gttctcgcag agcgttctgt
     8221 ttgtgatgtt cgaaaccaac accaacgaag ccagcgagta tttaagccag tatttcagcc
     8281 tgaaaattgt gcttatcgcg ctggcctata cggcaatggc agttctgctg tggacacgtc
     8341 tgcgcccggt ctatattcca aagccgtggc gttatattgt ctcttttgcc ctgctttatg
     8401 gcttgattct gcatccgatc gccatgaata cgtttatcaa aaacaagccg tttgagaaaa
     8461 cgttggataa cctggcctcg cgtatggagc ctgccgcacc gtggcaattc ctgaccggct
     8521 attatcagta tcgtcagcaa ctaaactcgc taacaaagtt actgaatgaa aataatgcct
     8581 tgccgccact ggctaatttc aaagatgaat cgggtaacga accgcgcact ttagtgctgg
     8641 tgattggcga gtcgacccag cgcggacgca tgagtctgta cggttatccg cgtgaaacca
     8701 cgccggagct ggatgcgctg cataaaaccg atccgaatct gaccgtgttt aataacgtag
     8761 ttacgtctcg tccgtacacc attgaaatcc tgcaacaggc gctgaccttt gccaatgaaa
     8821 agaacccgga tctgtatctg acgcagccgt cgctgatgaa catgatgaaa caggcgggtt
     8881 ataaaacctt ctggatcacc aaccagcaga cgatgaccgc ccgcaatacc atgctgacgg
     8941 tattttcgcg ccagaccgac aagcagtact acatgaacca gcaacgtacg cagagtgcgc
     9001 gtgaatacga caccaacgtg ctgaagccgt tccaggatgt gctgaatgac cctgcgccga
     9061 agaaactgat catcgttcat ctgctgggta cgcatatcaa atacaaatac cgctacccgg
     9121 aaaatcaggg caagtttgat ggcaataccg atcatgttcc gccaggatta agcgcagaag
     9181 agctggaatc atataacgat tatgacaacg ctaacctgta taacgatcat gtggttgcca
     9241 gcctgattaa agactttaaa gcggcagacc cgaacggatt cctggtttac ttctctgacc
     9301 acggtgaaga ggtttacgac acgccgccgc acaaaactca ggggcgtaac gaagacaacc
     9361 cgacgcgcca catgtacacc attccgttcc tgctgtggac gtcggaaaaa tggcaagcga
     9421 ctcatccccg tgatttctca caggatgtcg atcgtaaata cagcctggcg gaactgatcc
     9481 acacctggtc agatttggcg ggcttatctt acgacggtta cgatccaacc cgttcagtgg
     9541 tgaatccgca gttcaaagaa actacccgct ggattggtaa cccgtacaag aaaaacgcgc
     9601 tgatcgatta cgacactctg ccgtatggcg accaggtagg taatcagtaa atgacctttg
     9661 tatcgcgtct ggcgctattt gccggatgcg atactgccgc aatgtttaca tcgaactccc
     9721 ctcaggccac acttttcagg tggccttttt taataatgcc agttactgtt ttcccgtcta
     9781 catacctaag ggaataacat gtatcacgac gtcagctatc tgctctcccg cctgatcaac
     9841 ggcccgctgt ccctgcgcca gatttacttt gccagcagta acggtccggc gcccgatctt
     9901 gcgtatcagg tcgattttcc acggctggaa atagtgctgg aaggtgagtt tgttgatacc
     9961 ggcgctggag caacgttagt tcccggcgat gtgctgtacg ttcccgctgg tggctggaat
    10021 tttccacaat ggcaagcccc cgctactacc tttagcgtgc tgtttggcaa acagcaactc
    10081 ggcttcagcg tcgtgcaatg ggatggcaaa cagtatcaaa atctggcgaa gcaacacgtc
    10141 gcccgacgcg gcccgcgcat tggttctttt ctgctacaaa cgctcaatga aatgcaaatg
    10201 cagccacagg agcagcaaac ggcaaggctt atcgtcgcca gcctgcttag ccactgccgc
    10261 gatttgcttg gcagccaaat acagaccgcc tcacgcagcc aggctctatt cgaagctatt
    10321 cgtgattata tcgacgaacg ctatgcctcc gcgcttaccc gcgaatctgt cgcacaggcg
    10381 ttctatattt caccaaacta tctctcgcac ctgtttcaaa agacgggggc cattggtttt
    10441 aacgaatacc tgaatcacac gcgactggag cacgctaaga cgttattgaa aggctatgac
    10501 ctgaaggtaa aagaagtggc gcacgcctgc ggctttgtcg acagcaacta cttctgccgt
    10561 ctgtttcgta aaaacaccga acgctcgccg tcggaatatc gtcggcagta ccacagccaa
    10621 ctgacagaaa aaccgactac tccagaataa gatgggtttg ctgcggcgca gaaagcactt
    10681 tacgtaccgc gctcatcacc cgctgcggct cacgcaggaa cgcgtagata ctgcattgca
    10741 cgtagcgaca atgttcaaat cgctcggcac cagcaagctc gatatccgta atcaacagcg
    10801 caacatccgc ccggcggata tcctcgtccg ctaaacgatt ctccgttccc agcgcgccct
    10861 gagtttcaat gctgacgccc cacttctcta actggcacaa cttttccagc cgttccgccg
    10921 ccatataagt atgcgccacg ccactgacgc aggcggttac tgccaccagg tatgccattt
    10981 taacctccca cggtaacctg aaatccggcc cgttctgcca tttcgcgcat cgttgccaca
    11041 tcggctgacg acggcgcaga aacctctttc atcgaccatg ttttccccag cagacggtat
    11101 ttcggttcgc cgtactgatg aaacggtaac agatggatct gcctgatatt cagcgggatc
    11161 agcacgtcca gcgcctgctg catattctcc cggctgagtg tgaaaccggg gatcagcggc
    11221 aaacgcggga tcacgttgac accttcactc accagcaaac gcagattctc cagcacgcgt
    11281 ggcaggttca tcttcaccac atcccgcgcc tgagtcgcgt ccataatttt taaatcgaac
    11341 aacacttcat cgcacaattt cgccagcggt aacagcttgc tggctggcgc gtctccggca
    11401 gtttcgatgg cgcatgacac gccccacagc cgcagtcgct gtaaaaaacg ggtagcaaac
    11461 tcggcctgca ttaacacttc gccgccagaa agcgtgacgc cgccgccgga cgtgcgaaag
    11521 aaaatgtcat ctttcatcac ttcccgttcc agagcgtcaa ggctgatatc gcgaccaatc
    11581 cgttcaaacg caccggaggg gcattcatcc gcatcacgca aacatttcgc acagtgcaga
    11641 catttcgcct ctctgcgtac cgtctggatt ttgccggaga tcgactccgg attagcacac
    11701 cacgggcaaa gatgcggaca gcctttaaaa aagaccaccg tacgaatgcc ctcgccgtca
    11761 ttcagtgaat aacgctgaat gttgaaaatg cgcgccacat catcgcggcg cgtttccaca
    11821 acgttacagc tgatgcgctg tccggcggat gatgtcatcc tggatctcct tcgacagttc
    11881 gacaaagaag gcgctgtatc cggcaacgcg caccaccagc ccggcataat cttgtgggcg
    11941 ctgttgcgct tcccgcaacg tgtcggcgtt caccacgtta aactgaatat gctgcaactt
    12001 aagctgggta aacgcccgta agaagtcggc cagtttgcgt aatcccgctt caccttccag
    12061 ggtcgccgga gtgaatttca cgttcagcaa cgtaccgtta gacagcagcg tgttatcgag
    12121 cttactgact gacttcagta ccgctgttgg cccttgtgcg tcctggccca gcataggtga
    12181 caagccgccg tctgccagtt gttccccggc aaaacgaccg tctggcgtcg cgccaaccac
    12241 cgatcccaac gggacgtgag cagaaacggt atacgatccc ggcgtgaagt aaccgccgcg
    12301 cgggttctgg tatttttcca cttctttgca gtagtggcgt aacagttcgg cgctgatgtt
    12361 atccacctcg tcgatatcgt taccgtattt ctcgaagcgg ttaattaagc gagcacggac
    12421 tttttcgcct tctggcgttg caaagttggc ttttaatacc gacagcaatt cgtcaaaact
    12481 taaacgctgt tgctcaaaaa ccatcccttt caacgcatgg agagaatcgc tcaggttggc
    12541 gataccgatc ccctgtacgc cggagaagtt ataacgcgcg ccgccatcgg taatatcgcg
    12601 gcctttttcc agacaatcgc tgataaaaga cgagagcagc ggtacaggtg cccagtcgcg
    12661 atggccgata tcacaaatat tgctgccctc aaccatcagg gtgatgtagt ggctgatctt
    12721 ggcgcggatc tgctccagta aaccttcata agtcagcgca gcattgcctt cattttcata
    12781 caggcagatt tccatcactt tcagcagatt aaacatcgcg atgtcatgca agccgtaggt
    12841 tctgccggga atagataatt ccacacagcc cactacggaa tagtcgcgcg catcttccag
    12901 cgaaacgcca cggttgagga acgctggtac caccacttca tcgttaaaga tttgcggaat
    12961 accggtgccg aggcgaatgg tttcggcggt tttcatcagg aacggcgtgt cgatcaaggc
    13021 gttagtgcgc acgccgaggt tcggttgcgg taattgcacg ctttgataag cgtcaaggca
    13081 aaggaacgaa agcacgttca ccgcgctacg tccgttctcg gttaacccgc cgagcagtgc
    13141 ggtatagccg gtcgggaaac ctgcgaaata acgcgcgctg ctggtggagc gcaacagcac
    13201 gatgtcgttg catttcaccc ataaagattc gagtagttct ttcaggaacg ccggatcttc
    13261 gccctgggtt aaagatgcct gatagaacgg caacatatac tggtcgaagc gccccaacga
    13321 tagcgaactg gcgttggatt cgtattgcag aatgatgttc atgtaccaga ataactggca
    13381 cgcctgccag aacgtctgcg gcttatgttc ggcgttatgg cgggagatct ccgcaatagt
    13441 cagcagttct tcgcgacgct gggcatctgt gcagtttgcc gccattgttt ccgccagttc
    13501 agcgtaacgc agaatgtgtt tctgcgaggc ttccagcagt aacagcgcgg cctgataaaa
    13561 gtgattctcc ggctgttgct gacaatgttg ctgcatctgc gccaccagct cccccagccc
    13621 gtgattcagc aggcgtgggt aatcaataat aatgtgcccc tggcctttat ctgtctggtt
    13681 gatgctgaaa atctgcgtgc tggtcgcggc ttttacttca tctgtcatct gcccgttgat
    13741 gaaatctttc atcgaacgtt tttcccagta cgggaacaac tcttcacggt agatacgttt
    13801 atcttcttca ctgatagcaa agcggtcctg cggacgcgtc gggaattgat ccagctcttt
    13861 cagcagccag taagggtcca tttccggcga cataatccca gcgcgcggtt ttacggtgcg
    13921 gttaccggca atcagttctt catcacgaat cgaaatttca acatgttcaa ggatatacgc
    13981 tgttgctttc gcccggcgca atatcaccgg ttcgccttcg gtttgccgat ggctggcggt
    14041 ataaagcagc gcccgctcca gcgagatttc acgggtattg gaaaacagtg cagttttgag
    14101 gcgagagata cgattcgtca tgaggaactc ctgtgggaaa gcgaacccgc ccttgcgagc
    14161 gggagaagaa cttaagcggt ttgcgccagg tgagcttcaa ttttgctcat aatggcgtca
    14221 gcacgtttta ccgcatcgct gatgttgacg cgcacgatgg tcttgccagc aaaacgctct
    14281 tcaaacttga tgccgatatc tttggtcaga atcaccatat cggcgctcgc cacgtcttcc
    14341 gcagtcagtt cgttttccag accaattgac ccctgagttt ctactttcac ttcccagcct
    14401 ttcgctttcg cggcgctttc cagcgcctct gccgccatat aggtatgggc aacaccagat
    14461 ggacacgcgg ttactgcaat aattttcgtc atggctcgtt cctcaattaa ttaatttcaa
    14521 aatccaaatc caggtcgtct tccttctcat cagtcgaaga cccattttta cgcgccagac
    14581 ttttcagcac gttcacgcaa acagccgtca ccactgctcc gacagccact gctgcgatat
    14641 aacccagctt gccttcaacc accggcagca caatcagacc accccagcct gcatagcact
    14701 gcgcaccgac cagcgccgca gttaccgcac cacaaacaga accgaccatg atggaaggaa
    14761 tgacacgcag cggatcggca gcggcgaaag gaatcgcccc ttcagtaacc ccaacgcacc
    14821 ccatcaccag cgccgctttg ccggtttcgc gctcttctgc ggagaaattt ttacgaccaa
    14881 tcagcgtcgc cagccccatc cccagcggcg ggatacaaat acccacggca gcaatggcca
    14941 ccacggtata aacaccctga gcaacgcaaa tcagcatgaa ggcataggcc actttgttaa
    15001 ccggaccgcc catatcgaac gccagcatca gccccatgat caccgccagc ataacaatgc
    15061 tgccctgctg catcccctga agccactgag tcaggctgtt ggtcaacgcc cctacaggct
    15121 cgcccaagcc ccacatcatg atgcctgcgg taatcagtgt gccgatgata ggaataatga
    15181 agatgggcat caccgagcgc agaactttat gcaccggaat tttcttcagg taatgcacca
    15241 cgatgccgcc gataatcccg gcgatcagcg caccaaagaa gcccgcacca aagctgttac
    15301 caacccaggc accgatagcg cacggggcca gcgcagaacg ctctgcaatg gagtaaccga
    15361 tgtaagcggc gaggaaaggc accatcagcg tcaaacctgc aacgccgata tcaaacagtt
    15421 ttttcagatt tggatcggct acggcatccg gcactgcgcc tttgccatac aacatgacgg
    15481 aaaccgccag caaaataccg cccgatacca cgaagggaat catgtgtgaa acgcccgtca
    15541 ttaaatgctg acgggtattt ttcaggatct gcaccaactc attcataagc tgctcccggg
    15601 cttgcgaaag cccatgtcca tatttttgat ggaggaaaca ataggcaatc gcaggcggag
    15661 caaacagtgg ataaaaaggc catttactgg acttttgtaa tatcagtaca aaaatgcgat
    15721 ccgcctcata acttgcgata aaccggaagc atgccgcaaa acagcgtctt gtgagacgcg
    15781 tcgcaaattg acggttacat tacatttgtc cagcttttgg cagatttgtc acatggcagg
    15841 ccgttgccgt gcgctcttat cctgtaaaca gaaaaccgta ccgggagaga ggcaatggcc
    15901 ctgattgtgg aatttatttg tgagctacct aacggcgtac atgcacgtcc ggcaagccac
    15961 gttgaaacgc tgtgtaatac tttttcatca caaattgagt ggcataacct gcgcactgac
    16021 cgcaagggca acgccaaaag cgcccttgcg ctgattggca ccgatacgct ggcgggcgat
    16081 aactgccagt tactgatttc cggggccgac gaacaggaag cgcaccagcg tttaagccaa
    16141 tggctgcgcg atgaattccc ccactgcgat gcgccgctgg cggaagttaa atctgacgaa
    16201 ctggaaccac tgccggtttc actgaccaat ctgaatccgc aaattatccg cgcccgcacc
    16261 gtgtgcagcg gtagtgcagg cggcattctg acgccgatct cttctttaga tctcaatgcg
    16321 ctgggtaatc ttcccgcagc caaaggcgtt gacgccgagc aatccgcact ggaaaacggc
    16381 ctgacgctgg tactgaaaaa cattgagttt cgtctgctgg atagcgacgg tgccaccagc
    16441 gcgattctgg aagcgcaccg atccctggct ggcgatactt ccctgcgcga acatttactg
    16501 gcaggcgtca gcgccggatt aagctgcgcc gaagcgattg tcaccagtgc gaatcacttt
    16561 tgcgaagagt tcgcccgttc cagcagcagc tacctgcaag aacgtgccct ggacgtacgc
    16621 gacgtctgct tccagttact ccagcaaatc tacggtgagc aacgcttccc ggcaccgggc
    16681 aaactgacgc agcccgccat ttgtatggct gatgaactga ctcccagcca gttcctcgaa
    16741 ctggataaaa atcacctcaa aggtctgtta ctgaaaagtg gcggcaccac ctcacatacc
    16801 gtaattcttg cccgttcgtt caacattccg acactggttg gtgtagatat tgatgccctt
    16861 actccgtggc agcatcaaac gatttatatc gacggcaacg ctggggcgat tgtggttgag
    16921 ccaggagaag ccgtagctcg ttattatcag caagaagccc gcgtacagga cgccctgcgt
    16981 gagcaacagc gtgtctggct gacccaacaa gcccgtaccg ctgacggtat ccgcattgaa
    17041 attgccgcta acatcgctca ctccgtggaa gcgcaggccg cattcggcaa tggtgcggaa
    17101 ggcgttggtt tgttccgcac tgaaatgctc tatatggatc gcaccagtgc accgggcgaa
    17161 agcgagctgt acaacatttt ttgtcaggcg ctggagtctg ccaacggacg cagcattatt
    17221 gtgcgcacta tggacattgg cggcgacaaa cccgttgatt atctgaacat tccggcagaa
    17281 gctaacccgt tcctcggtta tcgcgctgtg cgtatttatg aagaatacgc ctcgctgttc
    17341 accacacaac tacggtcgat cctgcgcgcc tccgctcacg gcagcctgaa aatcatgatc
    17401 ccgatgatct cctcaatgga agagatctta tgggtgaaag aaaaactggc ggaagccaaa
    17461 cagcaactgc gtaacgaaca cattccgttt gatgagaaga tccagcttgg cattatgctg
    17521 gaagtaccgt cggtgatgtt catcatcgat caatgctgcg aagagattga tttctttagt
    17581 attggtagta atgacctgac gcaatatctg ctggcagtgg atcgcgataa cgctaaggtt
    17641 actcgtcact acaacagcct gaatccggca ttcttgcggg cgctcgatta cgccgtgcag
    17701 gcggtgcatc gccagggcaa atggattggt ctgtgcggtg agctgggagc gaaaggttcc
    17761 gtgctgccgt tgctggtcgg cttagggctg gatgaactca gcatgagcgc accatcaatt
    17821 ccggcggcga aagcgcggat ggcgcaactt gatagccgtg agtgccgcaa gttgctcaac
    17881 caggcaatgg cctgccgtac ttcgctggaa gtggaacacc tgctggcgca attccgcatg
    17941 acccaacagg acgcaccgct ggtcaccgcc gagtgcatca cgctggaaag tgactggcgc
    18001 agcaaagaag aagtgctcaa aggcatgacc gataacctgc tgctggcggg ccgctgccgc
    18061 tatccgcgta aactggaagc cgacttatgg gcgcgcgagg ccgttttctc taccggtctg
    18121 ggctttagtt ttgccattcc acacagcaaa tcagaacaca ttgagcaatc caccatcagc
    18181 gtggcgcgtc tgcaagcgcc ggtgcgctgg ggcgatgatg aagcgcaatt catcattatg
    18241 ttaaccctga acaaacacgc tgcgggcgat cagcacatgc gcattttctc gcgcctcgct
    18301 cgccgcatca tgcacgaaga attccgtaac gcgctggtta acgccgcctc tgccgacgct
    18361 atcgccagcc tgctgcaaca tgaactggaa ctgtaaaagg aaacatcatg gaactgtatc
    18421 tggacaccgc taacgtcgca gaagtcgaac gtctggcacg catattcccc attgccgggg
    18481 tgacaactaa cccgagcatt atcgctgcca gcaaggagtc catatgggaa gttctgccgc
    18541 gtctgcaaaa agcgattggt gatgagggca ttctgtttgc tcagactatg agtcgtgacg
    18601 cgcagggtat ggtggaagaa gcgaagcgcc tgcgcgacgc cattccgggc attgtggtga
    18661 aaatcccggt gacttccgaa ggtctggccg ccattaagat gctgaaaaaa gagggtatta
    18721 ctacgcttgg caccgcagtg tacagtgctg cacaagggtt actggccgcg ctggcagggg
    18781 caaaatacgt tgctccgtat gttaaccgcg tagatgccca gggcggggac ggcattcgta
    18841 cggttcagga gctgcaagcg ctgttagaaa tgcacgcacc agaaagcatg gtgctggcgg
    18901 ccagctttaa aacgccgcgt caggcgctgg actgtttact ggcaggatgt gaatccatca
    18961 ccctgccctt agatgtagcg caacaaatgc tcaacacccc tgcggtagag tcagctatag
    19021 agaagttcga acacgactgg aatgccgcat ttggcactac tcatctctaa aggagcaatt
    19081 atggaccgca ttattcaatc accgggtaaa tacatccagg gcgctgatgt gattaatcgt
    19141 ctgggcgaat acctgaagcc gctggcagaa cgctggttag tggtgggtga caaatttgtt
    19201 ttaggttttg ctcaaaccac tgtcgagaaa agctttaaag atgctggact ggtagtagaa
    19261 attgcgccgt ttggcggtga atgttcgcaa aatgagatcg accgtctgcg tggcatcgcg
    19321 gagactgcgc agtgtggcgc aattctcggt atcggtggcg gaaaaaccct cgatactgcc
    19381 aaagcactgg cacatttcat gggtgttccg gtagcgatcg caccgactat cgcctctacc
    19441 gatgcaccgt gcagcgcatt gtctgttatc tacaccgatg agggtgagtt tgaccgctat
    19501 ctgctgttgc caaataaccc gaatatggtc attgtcgaca ccaaaatcgt cgcgggcgca
    19561 cctgcacgtc tgttagcagc gggtatcggc gatgcgctgg caacctggtt tgaagcgcgt
    19621 gcttgctctc gtagcggcgc gaccaccatg gcgggcggca agtgcaccca ggcggcgctg
    19681 gcactggctg aactgtgcta caacaccctg ctggaagaag gcgaaaaagc gatgcttgcc
    19741 gccgaacagc atgtagtaac cccggcgctg gagcgcgtga ttgaagcgaa cacctatttg
    19801 agcggcgttg gttttgaaag cggcgggctg gcggcggctc acgcggtgca taacggcctg
    19861 accgctatcc cggacgcgca tcactattat cacggtgaaa aagtggcatt tggtacgctg
    19921 acgcagctgg ttctggaaaa cgcaccggtt gaggaaatcg aaaccgtagc tgcacttagc
    19981 catgcggtag gtttgccaat aactctcgct caactggata ttaaagaaga tgtcccggcg
    20041 aaaatgcgaa ttgtggcaga agcggcatgt gcagaaggtg aaaccattca caacatgcct
    20101 ggcggcgcga cgccagatca ggtttacgcc gctctgctgg tagccgacca gtacggtcag
    20161 cgtttcctgc aagagtggga ataacctact ccaaactccc ggcttgtccg ggagtttgaa
    20221 cgcaaaattg cctgatgcgc tacgtttatc aagcctacga aatctctgca atatattgaa
    20281 tatgcgtgct tttgtaggcc ggataaggcg ttcactccgc atccggcact ctgtgccagt
    20341 aatctgaaac tcccacgttg ttcaggagtt ttttatccgc ttctggacat tttctccaca
    20401 gaacaatcgt tagcccggaa ggtcgaatct gtgactctta ttttcacgga cgaaccagat
    20461 gaaagcttca ctggcgctgc tcagcctgct aacggcattt accagccact ccttaaaatc
    20521 tcccgccgtt ccaccaaccg tggtacaaat ccaggccaat accaaccttg ccatcgcaga
    20581 cggtgccaga cagcaaatcg gcagcacgct attttacgat ccggcgtata tgcagcttac
    20641 ctatcctggc ggtgatgtcc cgcaagaacg cggggtatgt tccgatgtgg tgatccgtgc
    20701 attgcgcagc cagaaggtcg atttgcagaa actggtgcac gaagatatgg cgaagaattt
    20761 tgccgagtac ccgcaaaaat ggcagttaaa gcgcccggac agcaacatcg atcaccgtcg
    20821 ggtgcctaat ctggaaacct ggtttacccg ccacgataaa acacgcccca tcagcaagaa
    20881 ccccagtgac tatcaagcgg gcgatattgt ctcctggcga ctggataacg gactggcaca
    20941 tattggggtg gtgtcagatg gcttcgcccg cgacggtacg ccgctggtga tacataacat
    21001 tggcgcaggc gcgcaggaag aagatgtgct gtttagctgg cggatggtgg ggcattatcg
    21061 ttattttgtt aaataagcga tcgccccaca tgaagaaaaa attcagatgc tttttacact
    21121 gacagcttct tttttcttac ggctcaccag cgccagtgcc agcacaatca gcacaatacc
    21181 gctaccttca acggcccccg gattctcacc gagcagccac caggaaaaga gtacgccgca
    21241 aacgggaacg gccagcgtgc ttaagctggc aatactggca ggcaagtttt tcaatacaaa
    21301 caaccataag ctccacgcca gcgccgtcgc cagaatcgca ctgtaggcca gcgcccagaa
    21361 cacggtgggc tgccagtcaa tttcacgttg cggcaccagt aaagcgacca cactcatcac
    21421 cagcgccgca tagagcatct gccaggatgt taacgataat aaatccacgc gcggatgacg
    21481 ggtgtatagg cgtttagcaa caatcgcgct cgccccccaa ctgacgccgg agaggattgc
    21541 cagcatggca cttttcattg aagagaaatc gagttgccac ggctgcaaca ccaaaaataa
    21601 gccgaaagcg gcaatcagta tcgcgaaata ttgcccacgt cgcaggcgtt caccgagaaa
    21661 caacgcggcg aaaatcacca cccagaacgg catggtatag cttaggatcg ccaccttccc
    21721 cgctccgccg ctgaccagcg cccactgcgc caggccaacc atcccgcagg tttgtaacag
    21781 ggcaatggct aaggtgtatt taaacggtgt cgggcgcatt ccgcgaccac gtaataaaag
    21841 gacgatgaat aaaacgagag cgccgaaaat gcagcgtaag gcggtaaagt cgaaggcacc
    21901 gatgtagctg gtgacttgct tcatgaaaat ccagctataa ctccagataa gtgtgagcac
    21961 aaccaggcca ctgattgcca gtgggttgct ctttcctgcg gcagacatga gagatccagt
    22021 gtgtagtttt cctgtggaca ctatacgccg gtaaagaaac ttcagcgact gcctgaaaga
    22081 cagtcgctga agaaggtcag attacagcag gtcgaaacgg tcgaggttca tcactttcac
    22141 ccatgccgcc acgaagtctt taacaaactt ctcgtgggca tcgctgctgg cgtaaacttc
    22201 cgctaccgca cgcaggacag agttagaacc aaataccaga tccgcacggc tggctgtgta
    22261 tttcacttcg ccggtttcac ggtcacgacc ttcgaacagc tctttcgatt cgtcagtcgc
    22321 tttccactcg taacgcatat ccagcaagtt cacgaagaag tcattgctca atacgccaac
    22381 gcggtcagtg aagacgccgt ttttgctgcc atcgaagttg gcacccagta cacgcatccc
    22441 gcccaccagc gcagtcattt ccggcgcggt cagcgtcagt tgctgtgctt tgtcgatcag
    22501 cagtgactcg gtggtggaaa cgtccagacg agcgcgatag ttacggaaac cgtcagcaat
    22561 tggctccagc agctcaaaca tctcaatgtc agtctgatcc tgacgcgcat caacgcgacc
    22621 cggcgcaaac ggtacatgaa tgctcaaacc tgcggcgctt gcggctttct caacaccaac
    22681 cacaccagcc agcacgatga tatccgccag cgaggcttta ccagactctt tctggatttt
    22741 ctccagaaca ggcagagcac gaacggctgc ggcgttcaca tcccagtcgc gctgcggcat
    22801 taatgccaga cgcgcaccgt tggcaccacc gcgtttgtcg ccaccacgga aggtagaagc
    22861 agatgcccag gccaccgata ccagctcact aacagacaga ccagaatccg caatcgcgaa
    22921 tttcagatcg ataatgtcct gctcggtcgg gttgtagatc ggctgcggca gcggatcttg
    22981 ccagatcaga tcttctttcg gtacttccgg tccgatgtaa cgagatttcg gccccatatc
    23041 cctgtgcgtc agtttgaacc aggcacgggc aaaggcttcg ttgaacgcct gcggatcgtt
    23101 gaggaaacga cgagagatct tctcgaactc aggatcaaaa cgcagcgtca ggtcggtcac
    23161 cagcatcgtc ggtttacgtt tcttcgacgg atcgaacgga tccgggataa tttccggtgc
    23221 gtctaccgct tcgaactgga ttgcgccagc cggactgcgg gtctgtaccc actcatattt
    23281 gaacaggttc tcgaagaaat agttgctcca ctgggtcggc gtctgggtcc agacaacttc
    23341 cagaccagag gtaatggcat ctgcgccaac gccgctgccg taagtgctcg cccagcctaa
    23401 accttgttct tcaatcggtg cagcttctgg atcaggacct acatttgatg tcggaccggc
    23461 accgtgggtt ttacccagcg tatgaccacc cgcaatcagc gccacggttt cttcgtcgtt
    23521 catgcccatg ttgccgaagg tcgcgcggat agctgctgcc gcagaaagcg gttcgccgct
    23581 gtggtccggg ccttccgggt taacgtaaat caggcccatc tcggttgcac ccagcggtgc
    23641 tttcgccagc gcttccggat gacggtgagt cagccaggct ttttcatcac cccagttaac
    23701 atccagatcc ggttcccaga cgtcttcacg accggcacca aaaccgaagg tacggaagcc
    23761 ggagttttcc agcgccacgt tacctgcgag gataaacagg tcggcccagg agattttctg
    23821 accatatttc tgtttgattg gccacaacag gcgacgcgct ttatcgaggc ttacgttatc
    23881 cggccaggag ttcagcggtg caaaacgttg ctgaccacga cccgcgccgc cgcgtccatc
    23941 gattgaacgg taagtacccg cgccgtgcca ggccatacga ataaacagac cggcgtaact
    24001 gccccagtcg gcaggccacc acggctgaga ttctgtcagc agggctttca gatctttttt
    24061 caggccgtag taatctaatt tgctgaattc tttgcggtag tcaaagtcct cacccagtgg
    24121 gttagaacga ttagaatgtt ggtttaacag gtcaacacgg agttgatttg gccaccagtc
    24181 gcgagtggtt gtgcccgccc ccgcgctctg gtcgtgaccg ccctgatgga acgggcattt
    24241 gccagtggct gtggtgttat gaatatcgtc tgacgtgctc atcaatgtgc tcccctctac
    24301 agtgttaccg ttacgataca cagcgttaga gagaagttat aattgaatta atccacggat
    24361 gcgatagttg agatcttaca tattgttggt taaagagatg tagatcaaat tgatcgtaat
    24421 taagcttttt tgattatgtc cgaattcgga caaatgcttt ataaaaaagg gttttaggtt
    24481 catatccttc aagtaatgag ggagaaaaca aggctgataa ccttattttc ccgccctcat
    24541 ttcgaggcag cattttgtgc tctgtttaaa atttgtgatc actgtgtgat tttcacaaaa
    24601 gccacactat ttataaacca ggtcgaacac ccagcgtatg gcaaatcgcg taactcattt
    24661 cagcgcggtt aagcgtatag aagtggaaat ctttcactcc ttcacggctt aaaatcttca
    24721 ccatatccat ggcaatattc gctcccacca gtttgcgggt ttcggcatcg tcgtccagcc
    24781 cgtcaaacat ttgtgccatc cacgccggaa tacgcacgtt ggtcatatcg gcaaatttct
    24841 tcgcttgttt aaagttcgag accggcaaaa ttcccggaat aatttccaca tcaatacccg
    24901 ccgatacgca gcggtcacga aaacgcaggt agctttcgac atcaaagaag aactgagtaa
    24961 tcgcgcggtt ggctccggca tcgactttgc gtttcaggtt gagcaaatcc gcctgagcgc
    25021 tttttgcttc cgggtgaacc tccggatacg ccgccacgga gatatcgaaa tctgccactt
    25081 cttttaacag cgtcaccagg tcagaagcat acatttccgg cttaccactt cccggcggca
    25141 gatcgccacg cagcgccacg atatgacgaa taccgttatt ccagtagtcg cgtgcaatgg
    25201 tgcgcaactc gtcgggcgtc gcatcaatgc aagtaagatg cggtgccgct tccagaccag
    25261 tgcgatcttt aatgccttta ataatgctgt gcgtacggtc gcgctcgccg gagttcgcgc
    25321 cataggtcac cgatacaaac ttcggtttca ggctgctaag gcgatcgatg gagttccaca
    25381 gggtctgctc catttcactg gtacgcggcg ggaaaaactc gaacgaaacg ttaatctgcc
    25441 cctggacttc tgccaggctc tgattcaggg catcccgctg gctggcgtga aaaaagctca
    25501 taccttacct catcaatcgc ttgtagttat atgttgtgtt gtgcacatct atccatttag
    25561 acgtccagat gtaaagatga aggaaaagcc gaagggcgtc aaccgaaaaa tgaccaatga
    25621 tggtgaggaa aaatcaggga agatgaaaaa acttcaggat ggtaagaaaa agaaaatccg
    25681 gtatgcaaag caaaccggac tgcaaatata aactaaccat gtacttattg cttcttgccg
    25741 gattcgtcgt aaacgcttca tccggcctaa tcctatgata attcaatata tttcattaga
    25801 aaccatagac ccgaaaacat gagtaccggg cattattaaa tttctgaaat tacaacaact
    25861 gtgccagccg gttgatatcc gactgaatcg ccccggcggt gacgtcgcgc cccgcgccag
    25921 gcccacggat caccagcggg ttgtcgcgat accagcggct ttcgatggca aagacgttat
    25981 cgcacggcag cagtgatgcc agcggatggt cttcacgcac cgcctccacg cccacacgcg
    26041 ctttgccgtt ggcatcgaaa cgcgccacgt agcgcagcac cagccccatt tcgcgggccg
    26101 cttccagccg ttgcaccatc tgctcgttaa gttcatcgcc attttcaaag aaatggtcga
    26161 tgctgccgcc ttcgcaatga gcaggcacca gcgattccac acgtacctgg tccggttcaa
    26221 tgttgtaacc cgcttcacgc gccagaatta ccagcttgcg cattacgtct ttgccggaga
    26281 ggtcgtcacg cggatcaggc tcggttaagc cctgctgcca cgcctgatcc accagctcgg
    26341 taaacggcac gctaccgtcg aattgcagga acagccagga gagcgtgccg gagaagatcc
    26401 cgctgatcga caaaatagta tcgccgctgt cgatcagatc gcgcacggtg tggttgatcg
    26461 gcaagcccgc accgacggtg gcattgtaca gccagtgacg cccggttttt tcgaaggcgt
    26521 cgtggatctg gcgatattta ttgctgtcgc tggctcccgc cagtttgttg gcgctgataa
    26581 cgtggaaacc gtggctggcg aaatcaagat actgatcagc aagctgctgg ctggcggtaa
    26641 cgtccagcac cactaaatca tcatacggat gggcgcgcat ccacaggaac aacgactctt
    26701 catcctgctc aaccgcttca tcgttgaaga aggctaacgc gcggctggcg tccagcccgt
    26761 catagctcaa caggctgcgg cggctgtcca ccacacctgc cagcacaaac tcaaagccgg
    26821 tacgtgccga aagcgtgctc tgctcacggg cgaacagttc cagccaacgg gaaccgatat
    26881 tgcccttacc gaacaatacc aggccgatgc gtttttctgc gcggaagacg gactgatgca
    26941 gcccctgaat caggctttcg gtcgggccgg tgcgcagtac tgccaccagg ctgatgccgt
    27001 catcggactg ccaggtaaat tcgaccggct ggcctttcag ttgctgccag aagcggtggc
    27061 aatgcagcgg gttacgggtg acgcctgcac cgaccatcgc caccagcgcc agcccctgac
    27121 gcaggcgcag ttcgccaggt aatcccgctt cgtcgaggat tttcagcgca ctgtcggcca
    27181 cttctgaggt gtagcaaaat tgcagcaact ggcgatcgtt atgtacgcca accgccagcg
    27241 ggcgtacctg cgcgcgtttc aggatttggt cgatctcttt atgcgccagt ttgaaatcct
    27301 gactggcggg cacctgaaac tcaatcaaac agacatcatc gtggctggtg acaatacgcg
    27361 caccagtacc ggaggccagc acgcgttcaa tgcgcgtgga accttgatcc ggcgtgtagc
    27421 tacagcgcag ttgcaggtcg atttcgctgc cagaaaccgg ctgtaaagta cgggcgtgaa
    27481 gaacgggagc cgccaggcgc gccagttcgc tggcctcatc cagacgcagc aacggcagca
    27541 ggcaggcatc tttcacttta cgcgggtcgg cactgtatac cccggcgacg tcgctccaga
    27601 tggttacgcg agaaacaccc gccagcgcac cgatttgtgt cgcggaatag tcggaaccgt
    27661 tacgccccag cagcaccgtt tcaccggcgt tgttgcggct gataaatccg gtcaccacca
    27721 gacgtttgcc cggatgttgc accagcagct gttgcagcaa cgggtaagaa agcccttcat
    27781 caacctgcgg ttgtgcggcg cgttcagcgc gtaaaaactc gcgggcatca agccaggccg
    27841 ctggcagccc ttgttgatta agtaccgcag acatcaggcg tgccgaccat acttccccgt
    27901 ggcccaccac ttccgcatac actgcgtcgt taataccgct gtcgagcagc gccgccaggc
    27961 gctcaaggtc gctgacaaaa gcgctaatga ggctatcggc ttcttcagcg ggtagcagac
    28021 cgctaatcag atcgcactga tagcgacgca gcgtttgttg aacctgatgc gcagagagac
    28081 gatcggtctg gcttagtttc aaccagttaa tcaactggtt agtggtgcta ccggcggcgg
    28141 aaaccaccat catatcgtca ggctgagagt actccgccat aatgcccgcg acacgcaaat
    28201 aacacttcac atcagccaga ctactgccac caaatttatg cagctgacga cctttcgccc
    28261 ctgcctgcgc aatcacactc atttttaccc cttgtttgca gcccggaagc cattttccag
    28321 gtcggcaatt aaatcttcgc catcttcaat accggtggag atacgcagca gcgtctcgga
    28381 aatcccggcg gcagcacgcg cttctggtgc catgcctgca tgtgtcatgg ttgcggcgtg
    28441 agagattaaa ctttccactc cccctaatga ttccgccagc gtaaacaacg acagcccgcc
    28501 caggaaacga cgcagcgtct gctcatcgcc atccagttca aaactcaaca ttgcgccaaa
    28561 gcctttttgc tgacgcgcgg caatttcatg cccctgattt tccggcagcg acgggtgata
    28621 cagttttttc accaacggct gggtttgcag atatttcaca atcgcctgcg cgttacgctg
    28681 ggccaactcc atgcgcggca ccagcgttcg caacccgcgt agcagcagat agctgtcaaa
    28741 cgcgccgccc gtcacgccaa tattgtttgc ccaccaggca agttcagtga caacgtccgg
    28801 gtctttagca atcaccacgc cggccaccac gtctgagtga ccgttcagat atttcgtgca
    28861 tgaatgcaac accagatcgg cacctaatgc cagcggattt tgtaatgccg ggcttaagaa
    28921 ggtgttatcc accacgctca ccgccccgac ttcccttgcc agatggcaga ttttcgcaat
    28981 atccacgacg cgtaacaatg gattacttgg gctttctacc agtaccagtt tgggtttttc
    29041 tgccagcgct gcccgtaatg cctgttcatc gccttgatca acaaacaaca cgcgatagca
    29101 accacgtttc gccaggctgt cgaacaggcg atagctaccg ccgtagcagt cgtgcggcgc
    29161 taccagcaga tcgccaggtt tcaaaaagac ggtcgttacc aggtgaatcg cggacatgcc
    29221 ggtattagta agtactgcac cagcaccgcc ttccagttct gccagtgctc gctgaaccac
    29281 atcgcgcgtt gggttgccac gacgcgagta atcatgcgcg cgcggttcat taaatccggt
    29341 aaagttatag gtactggaaa gatggatggg tgggacaacg caaccatact gttcgtcgtc
    29401 atttaacccg ctacgcactg cgatggtggc ctgtttacgc gtcatgtgat gatgttccct
    29461 gggctttatt ggtgaaatgt caggcaccag agtaaacatt gtggtaatgg acgtcaatac
    29521 atctggacat ctaaacttct ttgcgtatag attgagcaaa tcccaaatag ccgttaaaat
    29581 tatatgcatt atcacgccaa caggtggatt acccgatgta acggtagcgc ctgtacggta
    29641 aactatgcgg gtttacggcc agtgcccaca ttaactgtgt ggtctggtct caatttattg
    29701 acgaagagga ttaagtatct catggctgaa tggagcggcg aatatatcag cccatacgct
    29761 gagcacggca agaagagtga acaagtcaaa aagattacgg tttccattcc tcttaaggtg
    29821 ttaaaaatcc tcaccgatga acgcacgcgt cgtcaggtga acaacctgcg tcacgctacc
    29881 aacagcgagc tgctgtgcga agcgtttctg catgccttta ccgggcaacc tttgccggat
    29941 gatgccgatc tgcgtaaaga gcgcagcgac gaaatcccgg aagcggcaaa agagatcatg
    30001 cgtgagatgg ggattaaccc ggagacgtgg gaatactaaa gcggaaaaga gcggcgcgga
    30061 gtggaaccgc ctgatgcgct acgcttatca ggcctacgtc atattgcaat ttattgaatt
    30121 tgcacgaact tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgagcac
    30181 gttgtctgcg acccaccgct ttttatacat ggacgtttaa ctatgaaaaa caggctgctg
    30241 atcctcagcc tgctggtttc tgtacctgcc tttgcctggc agccgcaaac tggcgacatc
    30301 atctttcaaa tctctcgctc atcgcaaagt aaagcgatcc aactggcgac ccattccgat
    30361 tacagccaca ccggtatgct ggtgatgcgt aacaaaaagc cctacgtttt tgaagcggtc
    30421 ggcccggtga aatacacccc gctcaagcag tggattgccc atggtgaaaa gggcaaatac
    30481 gtcgttcgcc gcgttgaagg cggactgagt gttgaacaac agcaaaaact ggcgcaaacg
    30541 gcaaaacgtt atctcggtaa accgtatgat ttcagcttct catggagcga cgatcgccag
    30601 tactgttcgg aagtggtgtg gaaggtttac cagaacgcgc tgggaatgcg cgtgggcgaa
    30661 cagcagaagt tgaaagagtt tgatctcagc aacccgctgg tccaggcgaa gctcaaagaa
    30721 cgctacggta aaaatattcc gcttgaggaa acggtagttt caccacaagc cgttttcgac
    30781 gcgccacaac tcaccacggt tgccaaagag tggccgctgt tttcgtggta atggatgatg
    30841 aaaaaaataa ttcccgccgt agtctgtttg ttgctttgta gttgcacgac ctttccgcaa
    30901 gctgtcgctc cggtaaacgg tgatgcctcc aattagttga attaaggata taatactcgc
    30961 ttttaaggtt gtttcatggt cacagttaac cttcattgcc cccgttgcca gtccgttcag
    31021 gtttaccgtc acgggcagaa cccaaaaaga catgatcgat tccgcggccg tgactgtcac
    31081 cgggtatttc agctcagtta ctgttacgag ccccgtaaac cgggagttaa agaccaaatc
    31141 gctgaaatga ctttcaacgg tgccggggtt cgcgatactg cccgtacgct gaaaatcggc
    31201 attaatacag tcatccgaac tttaaaaaac tctcgccacg aagaataacg tcatccccgg
    31261 tcgctcatgc tgatgtggca ctcatctgca aacttagtca gcaatggagc tttgtcggca
    31321 gtaaagcgcg acagcactgg ctctggtacg tgtacaacac gaaaactggt ggtgttctgg
    31381 cctacacttt tggcccccgg actgacgaaa cctgtcgtga attgcgggcg ctgcttaccc
    31441 ttttaccatc ggcatgttga ccactgacga atggggtagt tacgctagag agttaccgaa
    31501 ggagaagcat ctgactggta agatttttac tcagcacatt gagcgtaaca atctgaccct
    31561 gagaacccga atcaaacgtc tggcccgcag aaccatctgt ttctcgcgtt ctgtagaact
    31621 tcatgaaaaa gtcatcggtg cttttatcga aaatatatgt tctactaatt ggagtcatca
    31681 cccttctgat aagctttcct tggatgatta ccagactgaa tacacagcca aagattttta
    31741 cctcattctg ctcttt
//
LOCUS       DAPGWD010000038        31740 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPGWD010000038 DAPGWD010000000
VERSION     DAPGWD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.038"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(23..910)
                     /gene="rarD"
                     /locus_tag="RHX88_001726"
     CDS             complement(23..910)
                     /gene="rarD"
                     /locus_tag="RHX88_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDV2230421.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(962..1429)
                     /locus_tag="RHX88_001727"
     CDS             complement(962..1429)
                     /locus_tag="RHX88_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDV2230422.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1594..2463
                     /gene="pldA"
                     /locus_tag="RHX88_001728"
     CDS             1594..2463
                     /gene="pldA"
                     /locus_tag="RHX88_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDV2230423.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2596..4425
                     /gene="recQ"
                     /locus_tag="RHX88_001729"
     CDS             2596..4425
                     /gene="recQ"
                     /locus_tag="RHX88_001729"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDV2230424.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQINSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4489..5109
                     /gene="rhtC"
                     /locus_tag="RHX88_001730"
     CDS             4489..5109
                     /gene="rhtC"
                     /locus_tag="RHX88_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDV2230425.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5171..5791)
                     /gene="rhtB"
                     /locus_tag="RHX88_001731"
     CDS             complement(5171..5791)
                     /gene="rhtB"
                     /locus_tag="RHX88_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDV2230426.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5902..6924
                     /gene="pldB"
                     /locus_tag="RHX88_001732"
     CDS             5902..6924
                     /gene="pldB"
                     /locus_tag="RHX88_001732"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDV2230427.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6932..7732
                     /gene="yigL"
                     /locus_tag="RHX88_001733"
     CDS             6932..7732
                     /gene="yigL"
                     /locus_tag="RHX88_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDV2230428.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            7808..8707
                     /gene="bioP"
                     /locus_tag="RHX88_001734"
     CDS             7808..8707
                     /gene="bioP"
                     /locus_tag="RHX88_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDV2230429.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8595..9548)
                     /gene="metR"
                     /locus_tag="RHX88_001735"
     CDS             complement(8595..9548)
                     /gene="metR"
                     /locus_tag="RHX88_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDV2230430.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9671..11932
                     /gene="metE"
                     /locus_tag="RHX88_001736"
     CDS             9671..11932
                     /gene="metE"
                     /locus_tag="RHX88_001736"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDV2230431.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLSEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(11972..12787)
                     /locus_tag="RHX88_001737"
     CDS             complement(11972..12787)
                     /locus_tag="RHX88_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV2230432.1"
                     /translation="MATTQQSGFTPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGQLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13049..13810
                     /gene="udp"
                     /locus_tag="RHX88_001738"
     CDS             13049..13810
                     /gene="udp"
                     /locus_tag="RHX88_001738"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDV2230433.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            13950..15377
                     /gene="rmuC"
                     /locus_tag="RHX88_001739"
     CDS             13950..15377
                     /gene="rmuC"
                     /locus_tag="RHX88_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDV2230434.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15472..16227
                     /gene="ubiE"
                     /locus_tag="RHX88_001740"
     CDS             15472..16227
                     /gene="ubiE"
                     /locus_tag="RHX88_001740"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDV2230435.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16241..16846
                     /gene="ubiJ"
                     /locus_tag="RHX88_001741"
     CDS             16241..16846
                     /gene="ubiJ"
                     /locus_tag="RHX88_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDV2230436.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16843..18483
                     /gene="ubiB"
                     /locus_tag="RHX88_001742"
     CDS             16843..18483
                     /gene="ubiB"
                     /locus_tag="RHX88_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDV2230437.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18562..18831
                     /gene="tatA"
                     /locus_tag="RHX88_001743"
     CDS             18562..18831
                     /gene="tatA"
                     /locus_tag="RHX88_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDV2230438.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18835..19350
                     /gene="tatB"
                     /locus_tag="RHX88_001744"
     CDS             18835..19350
                     /gene="tatB"
                     /locus_tag="RHX88_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDV2230439.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19353..20129
                     /gene="tatC"
                     /locus_tag="RHX88_001745"
     CDS             19353..20129
                     /gene="tatC"
                     /locus_tag="RHX88_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDV2230440.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20171..20953
                     /gene="tatD"
                     /locus_tag="RHX88_001746"
     CDS             20171..20953
                     /gene="tatD"
                     /locus_tag="RHX88_001746"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDV2230441.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(20950..21438)
                     /gene="rfaH"
                     /locus_tag="RHX88_001747"
     CDS             complement(20950..21438)
                     /gene="rfaH"
                     /locus_tag="RHX88_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDV2230442.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21605..23098
                     /gene="ubiD"
                     /locus_tag="RHX88_001748"
     CDS             21605..23098
                     /gene="ubiD"
                     /locus_tag="RHX88_001748"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDV2230443.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23144..23845
                     /gene="fre"
                     /locus_tag="RHX88_001749"
     CDS             23144..23845
                     /gene="fre"
                     /locus_tag="RHX88_001749"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDV2230444.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24037..25200)
                     /gene="fadA"
                     /locus_tag="RHX88_001750"
     CDS             complement(24037..25200)
                     /gene="fadA"
                     /locus_tag="RHX88_001750"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDV2230445.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25210..27399)
                     /gene="fadB"
                     /locus_tag="RHX88_001751"
     CDS             complement(25210..27399)
                     /gene="fadB"
                     /locus_tag="RHX88_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDV2230446.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPNTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSLLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDTIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEESIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27589..28920
                     /gene="pepQ"
                     /locus_tag="RHX88_001752"
     CDS             27589..28920
                     /gene="pepQ"
                     /locus_tag="RHX88_001752"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDV2230447.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28920..29534
                     /locus_tag="RHX88_001753"
     CDS             28920..29534
                     /locus_tag="RHX88_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDV2230448.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29573..31024
                     /gene="trkH"
                     /locus_tag="RHX88_001754"
     CDS             29573..31024
                     /gene="trkH"
                     /locus_tag="RHX88_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDV2230449.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31036..31581
                     /gene="hemG"
                     /locus_tag="RHX88_001755"
     CDS             31036..31581
                     /gene="hemG"
                     /locus_tag="RHX88_001755"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDV2230450.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7363 a         8106 c         8698 g         7573 t
ORIGIN      
        1 aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc gcatccatca
       61 caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg gcacccggtt
      121 tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg tactggaaaa
      181 agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac agcggtacgg
      241 tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt tgccccatat
      301 ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc agcagccaca
      361 tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt accagaccgt
      421 agaaggcaaa actaaatgcc agccccagcg cgataatagg tagcgaacca aaagtccaca
      481 gctggaccaa cacgccacat atcgccagaa tcaccgccag ccattgcatc cggcggaatc
      541 gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata aagtaaccaa
      601 ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc cagttgccac
      661 caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga atcagcgttt
      721 ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa aacgaccaga
      781 tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg aagtacgctg
      841 gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc cgcgtttgtt
      901 ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg cttttatgtc
      961 ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct tcattgtgta
     1021 attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg gtagcagtaa
     1081 aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc cccatccgcg
     1141 atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag gtacttccca
     1201 cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg tgcaaaatgc
     1261 tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt gcgaactctt
     1321 tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga taaacaaaca
     1381 tctcacccac taatttcagg gcttgttcag cagtcagtac ggcagacata tcatccttac
     1441 acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt ctactttagg
     1501 aagggataac taacggcaat ggagttaagt atgtaaaata gcccgcagaa aaacattcac
     1561 cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg ctggttgttg
     1621 ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa agaggtgcat
     1681 gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca tgacaatccg
     1741 ttcacgctct atccttatga caccaactac ctcatctaca cacaaaccag cgatctgaat
     1801 aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga agtaaagttt
     1861 cagttgagcc tggcatttcc actgtggcgt gggattttag gcccgaactc ggtgttgggt
     1921 gcatcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc ttcaccgttt
     1981 cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta ccgttttgca
     2041 ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg gcgttcagac
     2101 ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa cggtaactgg
     2161 ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc ggatatcacc
     2221 aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc ggtgctcagt
     2281 gcgaaaggac agtacaactg gaacaccggc tacggcggcg cggagttagg cttaagttac
     2341 ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg cgaatcgctc
     2401 atcgactata acttcaacca gacccgtgtc ggtgtggggg ttatgctaaa cgatttgttt
     2461 tgatgaacgg ttgagtggtt ggcaaatctg gaatccagca tccaggatta ccctctcaga
     2521 gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt tttatttcag
     2581 gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg agctaaacag
     2641 gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga aattatcgac
     2701 actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg aaaatccctt
     2761 tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc accgctgatt
     2821 tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc ggcgtgcctt
     2881 aactcgacgc aaacccgcga acagcaactt gaagtgatga caggctgccg caccgggcaa
     2941 attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct tgagcatctg
     3001 gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc ccaatggggc
     3061 cacgatttcc gcccggaata tgccgcgctc ggtcagttgc gccagcggtt cccgacgctg
     3121 ccgtttatgg cgctgaccgc cacagccgac gacaccacgc gccaggatat cgtgcgcctg
     3181 ctggggctga acgatccgct gattcaaatc aacagttttg accgtccgaa tattcgctac
     3241 atgctgatgg agaagttcaa accgctcgat cagttgatgc gctacgtgca ggaacagcgc
     3301 ggtaagtcag gcattatcta ctgcaacagc cgcgcgaaag tagaagacac cgctgcgcgc
     3361 ctgcaaagca agggaattag cgcggcggcc tatcatgccg ggctggaaaa taatgttcgc
     3421 gccgatgtgc aggaaaaatt ccagcgcgat gacctgcaaa ttgtggtggc gacggtggcg
     3481 ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga tattccgcgc
     3541 aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct gcctgcggaa
     3601 gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct ggaagagaag
     3661 ccgcaggggc agttgcagga tatcgagcgc cacaaactca atgcgatggg cgcgtttgcc
     3721 gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg gcgtcaggag
     3781 ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac aatacgatgg ttcaaccgat
     3841 gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat gggttatgtg
     3901 gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca tgacaaactg
     3961 aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt gatccgccag
     4021 ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct acaactgaca
     4081 gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt gccgcgtatc
     4141 gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga tcgcaaactg
     4201 ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt cccgccgtac
     4261 gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat caccgccagc
     4321 gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg taaaccgttt
     4381 atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc agcataaaaa
     4441 agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat gttgatgtta
     4501 tttctcaccg tcgccatggt gcacattgtg gcgcttatga gtcccggtcc cgatttcttt
     4561 tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg cgtgctgggt
     4621 attacctgcg gcgttatggt ttgggcaggg attgcgttgc ttggcctgca tttgattatc
     4681 gaaaaaatgg cctggctgca tacgctgatt atggttggcg gtggcctgta tctctgctgg
     4741 atgggttatc agatgctgcg cggcgcgctg aaaaaagagg cggtttctgc acctgcgcca
     4801 caggtcgagc tggcgaaaag tgggcgcagt ttcctgaaag gtttactgac caatctcgct
     4861 aatccgaaag cgattatcta ttttggttcg gtattctcat tgtttgtcgg tgataacgtt
     4921 ggcactaccg cgcgctgggg catttttgcg ctgatcattg tcgaaacact ggcgtggttt
     4981 accgtcgttg ccagcctgtt tgccctgccg caaatgcgcc gtggttatca acgtctggcg
     5041 aagtggattg atggttttgc cggggcgtta tttgccggat ttggcattca tttgattatt
     5101 tcgcggtgat gccagacgcg tcttcagagt aagtcggata aggcgtttac gccgcatccg
     5161 acattatttt tcacgcatgc ctcgccgatg ctaacagtgc tcccaccagc ataaacaacg
     5221 agccgaaaat cttattcagc gccttcatct gctttggtcc tttaatccat agagcaatcc
     5281 gttgagcaag ggtggcgtaa ccgatcatca caataatatc gaccacaata gtggtgacgc
     5341 cgagcacgat atactgcatc agttgcggct gttgcggcat gatgaattgc ggaaatagcg
     5401 ccgccagaaa cacaatactt ttgggattgg tgagattcac aaaaactgcg cgctggaaca
     5461 aatgtcgacg cgattgagta gaggccagcg atttaaggtc aattgcacca gcggcgcgcc
     5521 actgctggat tcccagccaa atcaagtaag ccgcgcctgc ccacttcaac acttcaaacg
     5581 caatcactga gcgggaaaat agcgtcccca accccacgcc aaccagcaca atatgaatcg
     5641 ccagtccggt ctgaagccca gcaatagacg ccaccgcgcc gcgataaccg tggttgagcg
     5701 aggtggtcat agtgttgatt gcaccagagc ctggcgacag gcttaaaatg atcgatgtca
     5761 gcaggtaggc aaaccaccat tctaaggtca tgatgaactc ccggtgtgtc tatttttgtg
     5821 ccacaatacg ctactgtcgc agcgttgtgt caggcacgct aaaaaaaacg attttacgtg
     5881 gtttaagagg cagattaccc gatgtttcag cagcaaaaag actgggaaac aagagaaaac
     5941 gcgtttgctg cttttaccat gggaccgctg actgatttct ggcgtcagcg tgatgaagca
     6001 gagtttactg gtgtggatga cattccggtg cgctttgtcc gttttcgcgc acagcaccat
     6061 gaccgggtgg tagtcatctg cccggggcgt attgagagct acgtaaaata tgcggaactg
     6121 gcctatgacc tgttccattt ggggtttgat gtcttaatca tcgaccatcg cgggcaggga
     6181 cgttccggtc gcctgttagc cgatccgcat ctcgggcatg ttaatcgctt taatgattat
     6241 gttgatgatc tggcggcatt ctggcagcag gaggttcagc ccggtccgtg gcgtaaacgc
     6301 tatatactgg cacattcgat gggcggtgcg atctccacat tatttctgca acgccatcca
     6361 ggtgtatgtg acgccattgc gctaactgcg ccaatgtttg ggatcgtgat tcgtatgccg
     6421 tcatttatgg cacggcagat cctcaactgg gccgaagcgc atccacgttt ccgtgatggc
     6481 tatgcaatag gcaccgggcg ctggcgcgcg ttgccgtttg ctatcaacgt actgacccac
     6541 agcagacagc gatatcgacg taacttacgc ttctatgctg atgacccaac gattcgcgtc
     6601 ggtgggccga cctaccattg ggtacgcgaa agtattctgg ctggcgaaca ggtgttagcc
     6661 ggtgcgggtg atgacgccac gccaacgctt ctcttacaag ctgaagagga acgcgtggtg
     6721 gataaccgca tgcatgaccg tttttgtgaa ctccgcaccg ccgcgggcca tcctgtcgaa
     6781 ggaggacgac cgttggtaat taaaggtgct taccatgaga tcctttttga aaaggacgca
     6841 atgcgctcag tcgcgctcca cgccattgtt gattttttca acaggcataa ctcacccagc
     6901 ggaaaccgct ctacagaggt ttaaatttct tatgtaccag gttgttgcgt ctgatttaga
     6961 tggcacgtta ctttctcccg accatacgtt atccccttac gccaaagaaa ccctgaagct
     7021 gctcaccgcg cgcggcatca actttgtgtt tgcgaccggt cgtcaccacg ttgatgtggg
     7081 gcaaattcgc gataatctgg agatcaagtc gtacatgatc acctccaacg gcgcgcgcgt
     7141 tcacgatctg gatggcaatc tgatttttgc tcataacctg gatcgcgaca ttgccagcga
     7201 tctgtttggc gtagtcaacg acaatccgga catcattact aacgtttatc gcgacgacga
     7261 atggtttatg aatcgccatc gcccggaaga gatgcgcttt tttaaagaag cggtgttcaa
     7321 atatgcgctg tatgagcctg gattactgga gccggaaggc gtcagcaaag tgttcttcac
     7381 ctgcgattcc catgaacaac tgctgccgct ggagcaggcg attaacgctc gctggggcga
     7441 tcgcgtcaac gttagtttct ctaccttaac ctgcctggaa gtgatggctg gcggcgtttc
     7501 aaaaggccat gccctggaag cggtagcgaa gaaactgggc tacagcctgc aagattgtat
     7561 tgcctttggc gatggaatga acgatgccga aatgctgtcg atggcgggta aaggctgcat
     7621 tatgggcagc gcgcatcagc gcctgaaaga tctgcatccc gagctggaag tgattggtac
     7681 taacgccgaa gacgcagtgc cgcattatct gcgtaaactc tatttatcgt aatcgttctt
     7741 tatttggtca gttgtcaacc tgatacttcg ctacaatgga tactcgttaa tcaaagagtt
     7801 ttccattgtg gcgctactta tcatcaccac gattctgtgg gccttctcct ttagctttta
     7861 tggcgagtac cttgcggggc acgtcgatag ctattttgcg gtgctggtgc gcgttggcct
     7921 ggcggcactc gtttttctgc cgtttctgcg tacccgtggc aatagcctga aaacagtcgg
     7981 cctgtatatg ctggtgggcg cgatgcagct tggcgtgatg tatatgctga gtttccgcgc
     8041 ttatctctat ctgacggttt ccgaactgct gctgttcacc gtactgacgc cgctctacat
     8101 cacgctgatt tatgacatca tgagtaagcg ccgtctgcgc tggggatatg cttttagtgc
     8161 cttgttggcg gtgattggtg ccgggattat tcgctatgat caggtcaccg accatttctg
     8221 gactggcttg ttgctggtgc aactctccaa tatcactttt gccatcggca tggtgggtta
     8281 caaacgcctg atggaaactc gcccgatgcc gcagcataac gcctttgcgt ggttctatct
     8341 tggcgcgttc ctggtggcgg taattgcatg gttcttgctg ggaaatgcgc agaaaatgcc
     8401 gcaaaccacg ttgcaatggg gcattctggt gtttcttggc gtggtggctt ccgggattgg
     8461 ctactttatg tggaactacg gcgcgacgca ggtggacgcc ggaacgctgg gaattatgaa
     8521 taatatgcac gttccggcag ggctgctggt aaacctggct atctggcacc aacagccgca
     8581 ctggccaacg tttattacag gcgcgctggt gatcctggcc tcactgtggg tgcatcgtaa
     8641 gtgggtcgct ccgcgctctt cacaaacggc agatgatcgc aggcgtgatt gcgcgctgag
     8701 cgaataaacg cttccgtaac tggctgacgc tgctcgccat cgcgcacggc ggcgtacagt
     8761 cggctccaca agccttcgcc cagggttttg gtcaccacca gaccctggcg ctcaaaactc
     8821 tctactaccc aatgcggtag cgcggcaata cccatccgcg cggcaaccat ctgaatcaac
     8881 aataaggtgt tatcgacgct ttttagtgac ggactgacgc ctgccggctg aagaaaatgc
     8941 cgccagacat ccagtcgact acgctgcacc ggataaatta atagcgtctc gctggcgaga
     9001 tcttccggtg taatacgcgt ttttgccgcc agtggatggt caggtgctaa caccagacgc
     9061 acttcatagt cgaacatcgg cgaataatgc agaccactgc gcggcagaat atcggacgtc
     9121 attaccagat ccagctcgcc ctgttgcaac gcgggctgcg ggtcaaacgt cacgcccgat
     9181 ttaaaatcca tctctacctg cggccagttc ttatggaaat tttctaacgc gggcgtcagc
     9241 cactgaatac agctatggca ctcaatggca atgcgcagac gcgtctgctg cggttcattg
     9301 caggcttgca gtgcctggct aatttgcggc agcacctggt ttgccagttg caacaggatt
     9361 tctccctgcg gtgtaaagcg tagcggctgg ctcttacgca caaatagccg gaagccaagg
     9421 cgttgttcca gatcgctaaa ctggtgagac agggcggatt gcgtctgatg caacgtcgcc
     9481 gcagcggctg cgagagagcc gcagttccgc aacgcttgta gcgttttcag gtgttttact
     9541 tcgatcatga aagtccttca cttcggcatg aataatttgc gcttgaggaa tatacagtaa
     9601 ccgccaatta tggatgtgta aacatctgga cggctaaata ctaaaaattc cataaggggc
     9661 atatcatata atgacaattc ttaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
     9721 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
     9781 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
     9841 cctgctgccg gtgggcgatt ttgcctggta cgatcatgtt ctgaccacca gtctgctgct
     9901 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
     9961 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    10021 atggtttaac accaactatc actacatggt gccggaattc gttaaaggcc aacagttcaa
    10081 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    10141 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    10201 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    10261 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    10321 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    10381 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    10441 gcctgttcag ggtctgcatg ttgacctcgt acatggtaaa gatgacgttg ctgaactgca
    10501 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    10561 gcgcgccgat cttagcgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    10621 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    10681 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactggc
    10741 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    10801 gattcaggca cgtcgtcact ctacccgcgt acataatccg gcggtagaaa agcgtctggc
    10861 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ccgaagccca
    10921 gcgtgcgcgt tttaaactgc cagcgtggcc gaccaccacg attggttctt tcccgcaaac
    10981 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    11041 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    11101 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    11161 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    11221 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    11281 gaagtatgcg caatcgctga ccgacaaacc ggtgaaaggg atgctgacgg ggccggtgac
    11341 catactctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    11401 tgcgctggcg ctgcgtgatg aggtggccga tctggaagcg gcagggattg gtatcatcca
    11461 gattgacgaa ccggcgctgc gcgaaggttt accactgcgt cgtagcgact gggacgccta
    11521 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    11581 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcca ttgcggcgct
    11641 ggatgcagac gtcatcacca tcgaaacctc gcgttccgac atggagttgc tggagtcgtt
    11701 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    11761 cgtaccgagc gtggaatgga ttgaagccct gctgaagaaa gcggcaaaac gcattccggc
    11821 agagcgcctg tgggtcaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    11881 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcgggggt aaaatccaaa
    11941 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
    12001 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
    12061 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
    12121 tagcagcccg cagcgcctgg cgcatggttt caacgctctc ctgcggaatg ctgttatcca
    12181 gaccgccata taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt
    12241 tcggtgaatt cagcgactta tcgcccgtca gtttgccgta ccacgccact gcggctttta
    12301 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
    12361 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
    12421 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
    12481 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
    12541 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
    12601 gcactacaat gaccactggc agttggccat cgctttgctt tggtctggca tggtaagcag
    12661 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
    12721 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgt aaatccagat tgttgtgttg
    12781 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
    12841 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
    12901 tgatttatgt gatttgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgccatg
    12961 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
    13021 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
    13081 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
    13141 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
    13201 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
    13261 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
    13321 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
    13381 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
    13441 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac
    13501 tcacgttggc gtgacagctt cttctgatac cttctaccca ggtcaggaac gttacgatac
    13561 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
    13621 cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gccagggcct
    13681 gcgtgccggt atggtggcgg gcgttatcgt taaccgcacc cagcaagaga tcccgaatgc
    13741 tgagacgatg aaacaaaccg aaagccaagc ggtgaaaatc gtggtggaag cggcgcgtcg
    13801 tctgctgtaa tcctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
    13861 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctatgttg
    13921 tgcgggtaag taatgaggct ggaggcgttg tggatttctc aatcatggtt tacgcagtta
    13981 ttgcgttggt gggtgtggca attggctggc tgtttgccag ttatcaacat gcgcagcaaa
    14041 aagccgagca attagctgaa cgtgaagaga tggtcgcgga gttaagcgcg gcaaaacaac
    14101 aaattaccca aagcgagcac tggcgtgcag agtgtgaatt gctcaataac gaagtgcgca
    14161 gcctgcaaag tattaacacc tctctggagg ccgatctgcg tgaagtaacc acgcggatgg
    14221 aagccgcaca gcaacatgct gacgataaaa ttcgccagat gattaacagc gagcagcgcc
    14281 tcagtgagca gtttgaaaac ctcgctaacc gtatttttga gcacagcaat cgccgggttg
    14341 atgagcaaaa ccgtcagagt ctgaacagcc tgttgtcgcc gctacgtgaa caactggacg
    14401 gtttccgccg tcaggttcag gacagcttcg gtaaagaagc acaagaacgc cataccctga
    14461 cccacgaaat tcgtaatcta cagcaactca acgcgcaaat ggcccaggaa gcgatcaacc
    14521 tgacgcgcgc gctgaaaggc gataacaaaa cccaggggaa ctggggtgaa gtggtactga
    14581 cgcgggtgct ggaggcttcc ggtctgcgtg aagggtatga atatgaaacc caggtcagca
    14641 tcgaaaatga cgcccgctcg cggatgcagc cggatgtcat cgtgcggttg ccgcagggaa
    14701 aagatgtggt gatcgacgcc aaaatgacgc tggtcgccta tgaacgctat ttcaacgccg
    14761 aagacgacta cacccgcgaa agcgcgctac aggaacatat cgcgtcggtg cgtaaccata
    14821 tccgtttgct gggacgcaaa gattatcaac agctgccggg gctgcgaact ctggattacg
    14881 tgctgatgtt tattcccgtt gaacccgctt ttttactggc gcttgaccgc cagccggagc
    14941 tgatcaccga agcgttgaaa aacaatatca tgctggttag cccgactacg ctgctggtgg
    15001 cgttgcgtac tattgccaac ctgtggcgtt atgagcatca aagccgcaac gcccagcaaa
    15061 tcgccgatcg tgccagcaag ctgtacgaca agatgcgttt gttcatcgat gacatgtccg
    15121 cgattggtca aagtctcgac aaagcgcagg ataactaccg ccaggcaatg aaaaaactct
    15181 cttcaggtcg cggaaatgtg ctggcgcagg cagaagcgtt tcgcggttta ggagtagaaa
    15241 ttaaacgcga gattaatccg gatttggctg aacaggcggt gagccaggat gaagagtatc
    15301 gacttcggtc ggttccggag cagccgaatg atgaagctta tcaacgcgat gatgaatata
    15361 atcagcagtc gcgctagccc attaggggta gttaagccgg gtagaaatct agggcatcga
    15421 cgcccaatct gttacacttc tggaacaatt ttttgatgag caggcattga gatggtggat
    15481 aagtcacaag aaacgacgca ctttggtttt cagaccgtcg cgaaggaaca aaaagcggat
    15541 atggtcgccc acgttttcca ttccgtggca tcaaaatacg atgtcatgaa tgatttgatg
    15601 tcatttggta ttcatcgttt gtggaagcga ttcacgattg attgcagcgg cgtacgccgt
    15661 gggcagaccg tgctggatct ggctggtggc accggcgacc tgacagcgaa attctcccgc
    15721 ctggtcggag aaactggcaa agtggtcctt gctgatatca atgaatccat gctcaaaatg
    15781 ggacgcgaga agctgcgtaa tatcggtgtg attggcaacg ttgagtatgt tcaggcgaac
    15841 gctgaggcgc tgccgttccc ggataacacc tttgattgca tcaccatttc gtttggtctg
    15901 cgtaacgtca ccgacaaaga taaagcactg cgttcaatgt atcgcgtgct gaaacccggc
    15961 ggccgcctgc tggtgcttga gttctcgaag ccaattatcg agccgctgag caaagcctat
    16021 gatgcatact ccttccatgt gctgccgcgt attggctcac tggtcgcgaa cgacgccgac
    16081 agctaccgtt atctggcaga atccatccgt atgcatcccg atcaggatac cctgaaagcc
    16141 atgatgcagg atgccggatt cgaaagtgtc gactactaca atctgacggc aggggttgtg
    16201 gcgctgcatc gtggttataa gttctgacag gagaccggaa atgcctttta aacctttagt
    16261 gacggcagga attgaaagtc tgctcaacac cttcctgtat cgctcacccg cgctgaaaac
    16321 agcccgctcg cgtctgctgg gtaaagtatt gcgcgtggag gtaaaaggct tttcgacgtc
    16381 attgattctg gtgttcagcg aacgccaggt tgatgtactg ggcgaatggg caggcgatgc
    16441 tgactgcacc gttatcgcct acgccagtgt gttgccgaaa cttcgcgatc gccagcagct
    16501 taccgcactg attcgcagtg gtgagctgga agtgcagggc gatattcagg tggtgcaaaa
    16561 cttcgttgcg ctggcagatc tggcagagtt cgaccctgcg gaactgctgg ccccttatac
    16621 cggtgatatc gccgctgaag gaatcagcaa agccatgcgc ggaggcgcaa agttcctgca
    16681 tcacggcatt aagcgccagc aacgttatgt ggcggaagcc attactgaag agtggcgtat
    16741 ggcacccggt ccgcttgaag tggcctggtt tgcggaagag acggctgccg tcgagcgtgc
    16801 tgttgatgcc ctgaccaaac ggctggaaaa actggaggct aaatgacgcc aggtgaagta
    16861 cggcgcctat atttcatcat tcgcactttt ttaagctacg gacttgatga actgatcccc
    16921 aaaatgcgta tcaccctgcc gctacggcta tggcgatact cattattctg gatgccaaat
    16981 cggcataaag acaaactttt aggtgagcga ctacgactgg ccctgcaaga actggggccg
    17041 gtttggatca agttcgggca aatgttatca acccgccgcg atctttttcc accgcatatt
    17101 gccgatcagc tggcgttatt gcaggacaaa gttgctccgt ttgatggcaa gctggcgaag
    17161 cagcagattg aagctgcaat gggcggcttg ccggtagaag cgtggtttga cgattttgaa
    17221 atcaagccgc tggcttctgc ttctatcgcc caggttcata ccgcgcgatt gaaatcgaat
    17281 ggtaaagagg tggtgattaa agtcatccgc ccggatattt tgccggtcat taaagcggat
    17341 ctgaaactta tctaccgtct ggctcgctgg gtgccgcgtt tgctgccgga tggtcgccgt
    17401 ctgcgcccaa ccgaagtggt gcgcgagtac gaaaagacct tgattgatga actgaatttg
    17461 ctgcgggaat ctgccaacgc cattcagctt cggcgcaatt ttgaagacag cccgatgctc
    17521 tacatcccgg aagtttaccc tgactattgt agtgaaggga tgatggtgat ggagcgcatt
    17581 tacggcattc cggtgtctga tgttgcggcg ctggagaaaa acggcactaa catgaaattg
    17641 ctggcggaac gcggcgtgca ggtgttcttc actcaggtct ttcgcgacag ctttttccat
    17701 gccgatatgc accctggcaa catcttcgta agctatgaac acccggaaaa cccgaaatat
    17761 atcggcattg attgcgggat tgttggctcg ctaaacaaag aagataaacg ctatctggca
    17821 gaaaacttta tcgccttctt taatcgcgac tatcgcaaag tggcagagct acacgtcgat
    17881 tctggctggg tgccaccaga taccaacgtt gaagagttcg aatttgccat tcgtacggtc
    17941 tgtgaaccta tctttgagaa accgctggcc gaaatttcgt ttggacatgt actgttaaat
    18001 ctgtttaata cggcgcgtcg cttcaatatg gaagtgcagc cgcaactggt gttactccag
    18061 aaaaccctgc tctatgtcga aggggtagga cgccagcttt atccgcagct cgatttatgg
    18121 aaaacagcga agcctttcct ggagtcgtgg attaaagatc aggtcggtat tcctgcgctg
    18181 gtgagagcat ttaaagaaaa agcgccgttc tgggtcgaaa aaatgccaga actgcctgaa
    18241 ttggtttacg acagtttgcg ccagggcaag tatttacagc acagtgttga taagattgcc
    18301 cgcgagcttc agtcaaatca tgtacgtcag ggacaatcgc gttattttct cggaattggc
    18361 gctacgttag tattaagtgg cacattcttg ttggtcagcc gacctgagtg ggggctgatg
    18421 cccggctggt taatggcagg tggtctgatc gcctggtttg tcggttggcg caaaacacgc
    18481 tgattttttc atcgctcaag gcgggccgtg taacgtataa tgcggctttg tttaatcatc
    18541 atctaccaca gaggaacatg tatgggtggt atcagtattt ggcagttatt gattattgcc
    18601 gtcatcgttg tactgctttt tggcaccaaa aagctcggct ccatcggttc cgatcttggt
    18661 gcgtcgatca aaggctttaa aaaagcaatg agcgatgatg aaccaaagca ggataaaacc
    18721 agtcaggatg ctgattttac tgcgaaaact atcgccgata agcaggcgga tacgaatcag
    18781 gaacaggcta aaacagaaga cgcgaagcgc cacgataaag agcaggtgta atccgtgttt
    18841 gatatcggtt ttagcgaact gctattggtg ttcatcatcg gcctcgtcgt tctggggccg
    18901 caacgactgc ctgtggcggt aaaaacggta gcgggctgga ttcgcgcgtt gcgttcactg
    18961 gcgacaacgg tgcagaacga actgacccag gagttaaaac tccaggagtt tcaggacagt
    19021 ctgaaaaagg ttgaaaaggc gagcctcact aacctgacgc ccgaactgaa agcgtcgatg
    19081 gatgaactac gccaggccgc ggagtcgatg aagcgttcct acgttgcaaa cgatcctgaa
    19141 aaggcgagcg atgaagcgca caccatccat aacccggtgg tgaaagataa tgaagctgcg
    19201 catgagggcg taacgcctgc cgctgcacaa acgcaggcca gttcgccgga acagaagcca
    19261 gaaaccacgc cagagccggt ggtaaaacct gctgcggacg ctgaaccgaa aaccgctgca
    19321 ccttcccctt cgtcgagtga taaaccgtaa acatgtctgt agaagatact caaccgctta
    19381 tcacgcatct gattgagctg cgtaagcgtc tgctgaactg cattatcgcg gtgatcgtga
    19441 tattcctgtg tctggtctat ttcgccaatg acatctatca cctggtatcc gcgccattga
    19501 tcaagcagtt gccgcaaggt tcaacgatga tcgccaccga cgtggcctcg ccgttcttta
    19561 cgccgatcaa gctgaccttt atggtgtcgc tgattctgtc agcgccggtg attctctatc
    19621 aggtgtgggc atttatcgcc ccagcgctgt ataagcatga acgtcgcctg gtggtgccgc
    19681 tgctggtttc cagctcgctg ctgttttata tcggcatggc gttcgcctac tttgtggtct
    19741 ttccgctggc atttggcttc cttgccaata ccgcgccgga aggggtacag gtatccaccg
    19801 acatcgccag ctatttaagc ttcgttatgg cgttgtttat ggcgtttggt gtctcctttg
    19861 aagtgcctgt ggcaattgtg ctgctgtgct ggatggggat tacctcgcca gaagacttac
    19921 gcaaaaaacg cccgtatgtg ctggttggtg cattcgttgt cgggatgctg ctgacaccgc
    19981 cggatgtctt ctcgcaaacg ctgttggcga tcccgatgta ctgtctgttt gaaatcggtg
    20041 tcttcttctc acgcttttac gttggtaaag ggcgaaatcg ggaagaggaa aacgacgctg
    20101 aagcagaaag cgaaaaaact gaagaataaa ttcaaccgcc cgtcagggcg gttgtcatat
    20161 ggagtacagg atgtttgata tcggcgttaa tttgaccagt tcgcaatttg cgaaagaccg
    20221 tgatgatgtt gtagcgcgcg cttttgacgc gggagttaat gggctactca tcaccggcac
    20281 taacctgcgt gaaagccagc aggcgcaaaa gctggcgcgt cagtattcgt cctgttggtc
    20341 aacggcgggc gtacatcctc acgacagcag ccagtggcaa gctgcgactg aagaagcgat
    20401 tattgagctg gccgcgcagc cagaagtggt ggcgattggt gaatgtggtc tcgactttaa
    20461 ccgcaacttt tcgacgccgg aagagcagga acgcgctttt gttgcccagc tacgcattgc
    20521 cgcagattta aacatgccgg tatttatgca ctgtcgcgat gcccacgagc ggtttatgac
    20581 attgctggag ccgtggctgg ataaactgcc tggtgcggtt cttcattgct ttaccggcac
    20641 acgcgaagag atgcaggcgt gcgtggcgcg tggaatttat atcggcatta ccggttgggt
    20701 ttgcgatgaa cgacgcgggc tggagctgcg ggaattgttg ccgttgattc cggcggagaa
    20761 attgctgatc gaaactgatg cgccgtatct gctcccgcgc gatctcacgc caaagccatc
    20821 atcccggcgc aacgagccag cccatctgcc ccatattttg caacgtattg cgcactggcg
    20881 tggagaagat gccgcatggc tggctgccac cacggatgcc aatgtcaaaa cactgtttgg
    20941 gattgctttt tagagtttgc ggaactcggt gttcttcaca ctgtgcttaa tctctttatt
    21001 aataagatta agcaatagca tggagcgagc ctcaccatcg ggttcggtga aaatggcctg
    21061 aaagccttcg aacgcgcctt cggtaataat caccttatca cccggataag gggttgccgg
    21121 atcgacaatg tctttcggtt tatataccga tagctgatga atcaccgccg atgggactat
    21181 cgctggcgac gcgccaaagc gcacgaagtg gctgacaccg cgggtcgcgt tgatagtcgt
    21241 ggtatgaatc acttctggat caaattcgac aaacaggtag ttgggaaaca atggctcact
    21301 gactgcagta cgttttccac gcacgatttt ttccagggtg atcatcggtg ccaggcaatt
    21361 cacagcctgt ctttcgaggt gttcctgggc acgttgaagt tgcccgcgct tgcagtacag
    21421 taaataccag gattgcataa tgactcttat ccgtttaatc ggggcgcaag gatagcaaaa
    21481 gctttacgct aagttaatta tattccccgg tttgcgttat accgtcagag ttcacgctaa
    21541 tttaacaaat ttacagcatc gcaaagatga acgccgtata atgggcgtag attaagaggc
    21601 tacaatggac gccatgaaat ataacgattt acgcgacttc ttgacgctgc ttgaacagca
    21661 gggtgagcta aaacgtatca cgctcccggt ggatccgcat ctggaaatca ctgaaattgc
    21721 tgaccgcact ttgcgtgccg gtgggcctgc gctgttgttc gaaaacccta aaggctactc
    21781 aatgccggtg ctgtgcaacc tgttcggtac gccaaagcgc gtggcgatgg gcatggggca
    21841 ggaagatgtt tcggcgctgc gtgaagttgg taaattattg gcgtttctga aagagccgga
    21901 gccgccaaaa ggtttccgcg acctgtttga taaactgccg cagtttaagc aagtattgaa
    21961 catgccgaca aagcggctgc gtggtgcgcc ctgccaacaa aaaatcgtct ctggcgatga
    22021 cgtcgatctc aatcgcattc ccattatgac ctgctggccg gaagatgccg cgccgctgat
    22081 tacctggggg ctgacagtga cgcgcggccc gcataaagag cggcagaatc tgggcattta
    22141 tcgccagcag ctgattggta aaaacaaact gattatgcgc tggctgtcgc atcgcggcgg
    22201 cgcgctggat tatcaggagt ggtgtgcggc gcatccgggc gaacgtttcc cggtttctgt
    22261 ggcgctgggt gccgatcccg ccacgattct cggtgcagtc acccccgttc cggatacact
    22321 ttcagagtat gcgtttgccg gattgctgcg cggtaccaag accgaagtgg tgaagtgtat
    22381 ctccaatgac cttgaagtgc ccgccagtgc ggagattgtg ctggaagggt atatcgaaca
    22441 aggtgaaacg gcgccggaag ggccgtatgg cgaccacacc ggttactata acgaagtcga
    22501 tagtttcccg gtatttaccg tgacgcatat tacccagcgt gaagatgcga tttaccactc
    22561 cacctatact ggtcgtccgc cggatgaacc cgcggtgctg ggtgtcgcac tgaacgaagt
    22621 gtttgtgccg attctgcaaa aacagttccc ggaaattgtc gatttttacc tgccgccgga
    22681 aggctgttct tatcgtctgg cggtagtgac gatcaaaaaa cagtacgccg gacacgcgaa
    22741 gcgcgtcatg atgggcgtct ggtcgttctt acgccagttt atgtacacta aatttgtgat
    22801 cgtttgcgat gatgacgtca acgcacgcga ctggaacgat gtgatttggg cgattaccac
    22861 ccgtatggac ccggcgcggg atactgttct ggtagaaaat acgcctattg attatctgga
    22921 ttttgcctcg cctgtctccg ggctgggttc aaaaatgggg ctggatgcca cgaataaatg
    22981 gccgggggaa acccagcgtg aatggggacg tcccatcaaa aaagatccag atgttgtcgc
    23041 gcatattgac gccatctggg atgaactggc tatttttaac aacggtaaaa gcgcctgatg
    23101 cgcgtttgtt ttgccctatt tatcgatccg acagagaaag cgcatgacaa ccttaagctg
    23161 taaagtgacc tcggtagaag ctatcacgga taccgtatat cgtgtccgca tcgtgccaga
    23221 tgcggccttt tcttttcgcg ctggtcagta tttgatggta gtgatggatg agcgcgacaa
    23281 acgtccgttc tcaatggctt cgacgccgga tgaaaaaggg tttatcgagc tgcatattgg
    23341 cgcttctgaa atcaaccttt acgcgaaagc agtcatggac cgcatcctca aagatcatca
    23401 aatcgtggtc gatattcccc acggagaagc gtggctgcgc gatgatgaag agcgtccgat
    23461 gattttgatt gcgggcggca ccgggttctc ttatgcccgc tcgattttgc tgacagcgtt
    23521 ggcgcgtaac ccaaaccgtg atatcaccat ttactggggc gggcgtgaag agcagcatct
    23581 gtatgatctc tgcgagcttg aggcgctttc gttgaagcat cctggtctgc aagtggtgcc
    23641 ggtggttgaa caaccggaag cgggctggcg tgggcgtact ggcaccgtgt taacggcggt
    23701 attgcaggat cacggtacgc tggcagagca tgatatctat attgccggac gttttgagat
    23761 ggcgaaaatt gcccgcgatc tgttttgcag tgagcgtaat gcgcgggaag atcgcctgtt
    23821 tggcgatgcg tttgcattta tctgagatat aaaaaaaccc gcccctgaca ggcgggaaga
    23881 acggcaacta aaactttatt cagtggcatt tagatctatg tcgcatccgg cattcggcac
    23941 acgatgcctg atgcgacgct ggcgcgtctt atcaggccta cattggtgcc ggatcggtag
    24001 accggataag gcgttcacgc cgcatccggc aagtggttaa acccgctcaa acaccgtcgc
    24061 aataccctga cccagaccga tacacatcgt cgccagacca aactggacgt ctttgcgctc
    24121 catcagattt agcagcgtgg tgctgatacg cgcaccggaa cagcccagcg gatgacccag
    24181 cgcgatcgcg ccaccgttga ggttgatctt ctcgtcaatc tgctccatta gccccagatc
    24241 tttaatacat ggcaggatct gcgcggcaaa ggcttcattc atttcaaaca cgccgatatc
    24301 actgacagaa agccccgctt ttttcagcgc cagcttcgag gccggaaccg ggccgtaacc
    24361 cataatcgat gggtcacaac caacgaccgc catcgaacgc acgcgggcgc gcggcttaag
    24421 acctaattca tgggcgcggc tttcactcat caccagcatg gcagctgcgc catcggaaag
    24481 cgcagaagat gtacccgccg ttaccgtacc gcttactgga tcaaacgccg gacgcagcgt
    24541 ggctagggct tccacggtgg tttccgggcg aatcacttcg tcgtaattaa actgcttcag
    24601 gacgccgtcg gcatcgtgac caccggtcgg gataatttca tttttaaatg cacccgactg
    24661 cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag gcatcctgca tttcacggct
    24721 gataccgtgc atacgcgcca gcatttctgc cgttaagccc atcatgcccg ccgctttggc
    24781 gacattgcgg ctcaggccgg ggtgaaaatc gacgccgtgg ctcattggca catggcccat
    24841 atgctccacg ccgccgacaa gacatgcctg cgcatcgcca gtcatgatca ttcgtgctgc
    24901 gtcatgcaat gcctgcatgg atgaaccaca caagcgattg acggtcaccg ccgggacaga
    24961 gtgtggtact tctgccagca gcgccgcgtt acgggcgata ttaaaaccct gctccagcgt
    25021 ctgctgcaca caaccccagt aaatatcgtc gagggccgcc gcttccagcg ccgggttacg
    25081 cgccagcagg ctacgcatta aatgagcgga gagatcttct gcacgcacgt tacgaaaagc
    25141 accgcccttc gaacggccca tcggggtgcg aattgcatcg acaatgacaa cctgttccat
    25201 tgtgactcct taagccgttt tcaggtcgcc aaccggacgg gctggctcaa ccgggggata
    25261 gtacggttcg ttatgacgcg ctttattacg cagaccttcc ggcacttcat acagcgggcc
    25321 gaggtgctga tattgctgtg ccatatcgag gtattttgcg ctaccgaggg tgtccagcca
    25381 gcggaacgcg ccgccgtgga acggagggaa gcccaggccg tagaccagcg ccatatccgc
    25441 ttctgccgga gtggcgataa tgctttcttc cagacagcgc accacttcgt tgaccatcgg
    25501 gatcatcatg cgggcgataa tctcttcttc gctgaaatcg cgcttcggct ggctcacttc
    25561 tgccagcagg tcgtcaacga cggcgtcttc ttctttcttc ggcttacctt tgctgtcttc
    25621 tttataacgc cagaaaccga ggccgttctt ctgaccaaag cggttggcat caaacagcgc
    25681 gtcgatggta tcgcgataat ctttctgcat ccgctgcggg aagcctgctg ccatgacagc
    25741 ctgggcatga tgcgcggtgt caatgcccac gacatccagc agatatgccg gacccatcgg
    25801 ccagccaaac tgtttttcca ttactttgtc gatcttgcgg aaatccgcgc cgtcgcgcag
    25861 caacaggctg aaaccggcga aatacgggaa cagcacgcgg ttgacgaaga agccggggca
    25921 gtcgttaacc acaatcggcg ttttgcccat cttgctcgcc caggcgacga ctttcgcgat
    25981 ggtttcgtcg gagcttttct cgccgcgaat tatttctacc aacggcattc ggtggaccgg
    26041 gttaaagaag tgcatcccgc agaagttttc tggacgttcc agcgcgttgg ccagttcgct
    26101 gataggaatg gttgaagtgt tagacgccag cacggtattc gggcgtactt tctgctcggt
    26161 ttctgccagt acggcttttt tcacttttgg gttttcgaca accgcttcta ccacaacatc
    26221 cacgcggtca aacccggcgt agtcgagcgt tgggtggatt gtggagatca cgccagccag
    26281 tttcagacca tcgatcttgc cgcgctcaag ctgcttgttc agcagtttcg cggcttcggt
    26341 catgccgagg gttaacgact tgtcgttgat atctttcatg acaaccggca cgcctttcca
    26401 cgcagactgg taagcgatgc cgccgcccat aatgcctgca cccagcaccg cggcctgttt
    26461 cggggtttca acgtctttgg tgagtttctt cgctttgcct tttacatatt gatcgttaag
    26521 gaaaatgccg accagtgcgc gggcttcgtt ggtatgcgcc agcgggacaa aacttttgtt
    26581 ttccaggttt aaggcttctt cacgaccaaa acgggccgca gcttcaatgg tttttactgc
    26641 ggtgatgggg gccggataat gtttccccgc tgtttgtgca accatccctt tagcgatggt
    26701 gaagctcatg gtggcttcaa tcttgctcag ttttagtggt tccagcttcg gctgacgttt
    26761 tgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa accgcctttg cgccttcaac
    26821 cagtttttct gctttgacta cgccatccac cagaccgatt ttcagcgcct gatccgcgcc
    26881 gacatcttta ccggcggcaa tgatttccag cgcactgtca gcgcccagca tacgtggcat
    26941 acgtacagaa ccgccaaagc ccggcatgat gcccagtttg gtttccggca gaccgatgcg
    27001 cagatccggc gtcgccagac gataatcggt cgccagcacg cattcgcagc caccgcccag
    27061 cgcatagccg ttgacggcag caatggtcgg caccggcaga tcttccaggc gattaaacac
    27121 gctattggca aagtgcagcc actgacttaa ctgttcttca ggaacgagga acagggacaa
    27181 aaattcggtg atatcagcac cgacgataaa ggctgctttg ttcgaacgca gcagcagccc
    27241 ttttagatct gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg cgacggtcgc
    27301 agtgtcgagt ttattaactg aacctggggc atcaaatacc agttcggcaa tgccatcttc
    27361 cagccagtca aggtacaggg tgtcgccttt gtaaagcatg tcagtctcct gaatccgcaa
    27421 ggtgatctgg tcgtaccaga tgcatcgaag tgtgtatttt gtgttaaaaa tatgcaaata
    27481 aaagattaaa gaaatgccga tctgatcacg ctcggcagaa atcacgctct ggatgaacga
    27541 tgtgctaaga tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat ggaatcactg
    27601 gcctcactct ataaaaatca tatagctacc ttacaggaac ggactcgcga tgcgctggcg
    27661 cgcttcaagc tggatgcgtt acttattcac tccggcgaac tgttcaacgt ttttctcgac
    27721 gatcatccct atccgtttaa agtgaacccg caattcaaag cgtgggtgcc ggtaactcag
    27781 gtgccaaact gctggctgct ggtggatggc gtgaacaagc cgaaactgtg gttctatctg
    27841 ccggttgatt actggcacaa cgtcgaaccg ctgccgacct ctttctggac tgaagacgtg
    27901 gaagtgatcg cgctgccgaa agccgatggc attggtagcc tgctgcctgc tgcgcgcggg
    27961 aatatcggtt atatcggtcc ggtgccggaa cgtgcgctgc aactgggtat tgaggccagc
    28021 aatatcaacc cgaaaggggt gatcgactac ctgcattact accgctcctt caaaaccgag
    28081 tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg tcaacggtca tcgcgcggca
    28141 gaagaagcgt tccgttctgg catgagcgag ttcgatatca acattgccta tctgaccgcg
    28201 accggtcatc gtgataccga cgtaccttac agcaacattg tggcgctcaa cgaacacgct
    28261 gcggtgttgc attacaccaa actggaccac caggcaccgg aagagatgcg cagcttcctg
    28321 ctggatgccg gggccgaata taacggctat gcggctgacc tgacccgtac ctggtcggca
    28381 aaaagtgaca acgactacgc acagctggtg aaagacgtaa atgatgaaca actggcgctg
    28441 atcgctacca tgaaagcagg cgtcagctat gtggattacc acatccagtt ccatcagcgc
    28501 atcgccaaac tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga agcgatggtc
    28561 gaaaacgatc tcaccggacc gtttatgccg cacggtattg gccatccgct gggcctgcag
    28621 gtgcatgacg tagccggttt tatgcaggat gatagcggta cgcacctcgc ggcaccggca
    28681 aaatatccgt acctgcgctg cacccgtatt ctccagccgg gcatggtgtt aaccatcgaa
    28741 ccgggtatct acttcattga atcgctgctg gcaccgtggc gtgaagggca gttcagcaag
    28801 cacttcaact ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg tatcgaagac
    28861 aacgtggtga tccacgaaaa caacgtggaa aacatgaccc gggatctgaa actggcgtga
    28921 tggaaagctg gttaattcct gcggcaccgg tcacggtcgt tgaagagatc aaaaaaagcc
    28981 gcttcataac gatgttggcg cataccgatg gcgttgaggc ggcgaaagcg tttgttgaat
    29041 cggtgcgggc agaacacccc gatgcccgcc accattgcgt ggcgtgggtg gcgggtgcgc
    29101 cggatgattc tcaacagctg ggtttctctg acgacgggga gccggcggga acggcaggta
    29161 aaccgatgct cgcccagcta atgggcagcg gcgtcgggga aattaccgct gtggtagtgc
    29221 gctactacgg cggcatattg cttggcaccg gtgggttagt gaaagcgtat ggcggcggcg
    29281 tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac gccattaacc gaatatactt
    29341 tgcaatgtga atatagtcag ttaaccggca ttgaagcgtt gctggggcag tgtgacggca
    29401 aaattatcaa cagtgattat caggcattcg ttctgctgcg ggtggcgctt ccggcggcga
    29461 aagtggctga attttccgca aagctggcgg attttagccg tggttcattg caattgttag
    29521 cgattgaaga ataatcccca cttcgttttg cagaactaag gaagcggcag agatgcattt
    29581 tcgcgccatt acccgaatcg ttggactact ggtcatctta ttttcaggga ccatgattat
    29641 ccctgggctg gtagcactca tctaccggga tggagcgggc cgcgctttta cccagacctt
    29701 ttttgtcgcc ctcgccattg gctctatgct gtggtggccg aaccgcaaag agaagggcga
    29761 actgaaatcc cgtgaggggt ttctgatagt ggtgctgttt tggaccgtgc tgggtagcgt
    29821 cggtgcgctc ccttttatct tctcggaaag cccgaacctc acgattaccg atgcgttttt
    29881 tgaatctttc tctggcctga ccaccaccgg ggccactacg ctggtggggc tggattcgct
    29941 ccctcatgcc atcctctttt atcgccagat gctgcaatgg tttggcggga tggggatcat
    30001 cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt gggatgcagc tctatcgcgc
    30061 agaaatgccc ggcccgctga aagataacaa aatgcgcccg cgaattgcgg aaacggcgaa
    30121 aaccctgtgg ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt tgtggtttgc
    30181 cggaatggat gcctttgatg ccatcggcca tagctttgcg actatcgcta ttggcggctt
    30241 ctcgacacat gatgccagta tcggttattt cgacagcccg actattaaca ctatcattgc
    30301 tatcttcctg ctgatctccg gctgtaacta cggtctgcac ttttcactgt taagtgggcg
    30361 tagtctgaag gtttattggc gcgatccgga atttcgcatg tttatcggcg tacagtttac
    30421 gctggtggtt atttgtacac tcgtactgtg gtttcataat gtctacagtt cggcgctgat
    30481 gacaattaac caggcgtttt tccaggtggt atcgatggcg acaaccgccg ggtttacgac
    30541 tgacagcatt gcccgctggc cgctcttttt gccggtactg cttttatgtt cagcttttat
    30601 cggcggttgc gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc tgctgctgtt
    30661 taagcagggg aaccgtgagc tgaaacgact ggtgcatccg aacgccgtct atagcattaa
    30721 gctggggaat cgcgcactgc cggaacgtat cctcgaagcc gtttggggat ttttctccgc
    30781 ctatgcattg gtgtttattg tcagtatgct ggcgattatc gccacgggcg tggatgactt
    30841 ttctgccttt gcctcagttg ttgcgacatt gaataacctg gggccagggc ttggcgtggt
    30901 tgctgataac tttaccagta tgaacccggt ggctaaatgg atcctgattg ccaacatgct
    30961 gtttggtcgt ctcgaggtct ttacattgct ggtgctcttt accccgactt tctggcgtga
    31021 atgatggagt aatacgtgaa aacattaatt cttttctcaa caagggacgg acaaacgcgc
    31081 gagattgcct cctacctggc ttcggaactg aaagaactgg ggatccaggc ggatgtcgcc
    31141 aatgtgcacc gcattgaaga accacagtgg gaaaactatg accgtgtggt cattggtgct
    31201 tctattcgct atggtcacta ccactctgcg ttccaggaat ttgtcaaaaa gcatgcgacg
    31261 cgactgaatt cgatgccgag cgccttttat tccgtaaacc tggtggcgcg caaaccggag
    31321 aagcgtactc cacagaccaa cagctacgcg cggaagtttc tgatgaactc gcaatggcgt
    31381 cccgatcgct gtgccgtcat tgccggggcg ctgcgttacc cacgttatcg ctggtacgac
    31441 cgttttatga tcaagctgat tatgaagatg tcaggcggtg aaacggatac gcgcaaagaa
    31501 gttgtctata ccgattggga gcaggtggcg aatttcgccc gagaaatcgc ccatttaacc
    31561 gacaaaccga cgctgaaata agcataaaga ataaaaaatg cgcggtcaga aaattatttt
    31621 aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca
    31681 acggcaaaca cgccgccggg tcagcggggt tctcctgaga atctcaacag agaaaagcaa
//
LOCUS       DAPGWD010000039        30860 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPGWD010000039 DAPGWD010000000
VERSION     DAPGWD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.039"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            248..631
                     /gene="secE"
                     /locus_tag="RHX88_001756"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="RHX88_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDV2230451.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="RHX88_001757"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="RHX88_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDV2230452.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="RHX88_001758"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="RHX88_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDV2230453.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="RHX88_001759"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="RHX88_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDV2230454.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="RHX88_001760"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="RHX88_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDV2230455.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="RHX88_001761"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="RHX88_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDV2230456.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="RHX88_001762"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="RHX88_001762"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDV2230457.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYVDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="RHX88_001763"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="RHX88_001763"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDV2230458.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12676..13215
                     /locus_tag="RHX88_001764"
     CDS             12676..13215
                     /locus_tag="RHX88_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HDV2230459.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="RHX88_001765"
     CDS             complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="RHX88_001765"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDV2230460.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="RHX88_001766"
     CDS             complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="RHX88_001766"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDV2230461.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="RHX88_001767"
     CDS             complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="RHX88_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDV2230462.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="RHX88_001768"
     CDS             complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="RHX88_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDV2230463.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="RHX88_001769"
     CDS             complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="RHX88_001769"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDV2230464.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="RHX88_001770"
     CDS             complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="RHX88_001770"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDV2230465.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19070..19170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="RHX88_001771"
     CDS             complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="RHX88_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDV2230466.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19793..20566
                     /gene="nudC"
                     /locus_tag="RHX88_001772"
     CDS             19793..20566
                     /gene="nudC"
                     /locus_tag="RHX88_001772"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDV2230467.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20606..21670
                     /gene="hemE"
                     /locus_tag="RHX88_001773"
     CDS             20606..21670
                     /gene="hemE"
                     /locus_tag="RHX88_001773"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDV2230468.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21680..22351
                     /gene="nfi"
                     /locus_tag="RHX88_001774"
     CDS             21680..22351
                     /gene="nfi"
                     /locus_tag="RHX88_001774"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDV2230469.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22394..22984
                     /locus_tag="RHX88_001775"
     CDS             22394..22984
                     /locus_tag="RHX88_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDV2230470.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23171..23443
                     /gene="hupA"
                     /locus_tag="RHX88_001776"
     CDS             23171..23443
                     /gene="hupA"
                     /locus_tag="RHX88_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDV2230471.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23456..24151
                     /locus_tag="RHX88_001777"
     CDS             23456..24151
                     /locus_tag="RHX88_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDV2230472.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="RHX88_001778"
     CDS             complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="RHX88_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDV2230473.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24816..26192
                     /gene="zraS"
                     /locus_tag="RHX88_001779"
     CDS             24816..26192
                     /gene="zraS"
                     /locus_tag="RHX88_001779"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDV2230474.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26189..27514
                     /gene="zraR"
                     /locus_tag="RHX88_001780"
     CDS             26189..27514
                     /gene="zraR"
                     /locus_tag="RHX88_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDV2230475.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27511..28800)
                     /gene="purD"
                     /locus_tag="RHX88_001781"
     CDS             complement(27511..28800)
                     /gene="purD"
                     /locus_tag="RHX88_001781"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDV2230476.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28812..30401)
                     /gene="purH"
                     /locus_tag="RHX88_001782"
     CDS             complement(28812..30401)
                     /gene="purH"
                     /locus_tag="RHX88_001782"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDV2230477.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7297 a         7985 c         8432 g         7146 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatatt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttcta agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agactccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgta tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatgtt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc gggttaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gggcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcacg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaatactg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagta ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggtca gaccattact cgtcagacag acgaattgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt acagcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagca cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt
    12541 cagaatattt aaggaatttc tgaatactca taatcaatgt agagatgact aatatcctga
    12601 aactgactga actaattgag tcaaactcgg caaggattcg atactattcc tgtgtaactt
    12661 tcttaaggaa cgagaatgaa acaggaagtg gaaaagtggc gaccttttgg acatccggat
    12721 ggtgatattc gtgatttatc atttcttgat gctcatcagg ctgtctacgt tcagcatcat
    12781 gagggcaaag agcctttaga gtatcgcttt tgggttacct actctcttca ctgcttcaca
    12841 aaagattatg aacatcagac gaacgaagaa aaacaatcgt taatgtacca cgcgcctaaa
    12901 gaatctcgtc ccttctgcca gcaccgttat aacttagcgc gcacacactt aaaaagaact
    12961 attttggcgc tgccagaaag caacgttatt catgccgggt atggtagcta tgccgtgatt
    13021 gaggtggact tagacggagg agataaggca ttttactttg ttgcgttcag ggctttcagg
    13081 gaaaagaaaa aactccgttt gcatgtaact agcgcttatc ccatttctga aaaacagaaa
    13141 ggtaaatcag tgaaattttt caccattgcc tacaacttat tgagaaataa gcagcttcct
    13201 cagccctcaa aataacaaaa cccaccttaa ggtgggtttc gccagagaat tatctctggt
    13261 attcagaacg ccattaccgg actttgcctt gaccttgcga taatcgcagg ttgcgggatg
    13321 tctgaatttc ttcagtctgc tgcatcctgg aagatgagaa catgtgttct tattttcgtc
    13381 tctatcatag ttgagtattt actctcttac aatcagatct ctttcattgc tcaacaggcg
    13441 atggcttcag actttgcatt acggaatttt taagaaaggc agggcgaaac gaggaagaag
    13501 cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca caacgctttc
    13561 aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt tcttacattc
    13621 cgtctcatag tctttgcgag gcccgtccca gatagctgtc ccagtctttc cataccggct
    13681 gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg
    13741 agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt ttcgagaagg
    13801 cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg
    13861 tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta
    13921 actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg cgcaggcgcg
    13981 gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc aaatgttcgg
    14041 caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg ccgaggccta
    14101 tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag aagaagtcct
    14161 gtttttttcc tttcagatga tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa
    14221 ccatcacgcc atccagacca agctgtttta actcggcgta ttccgtctcc gccagcggtt
    14281 gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga cgacgaaagt
    14341 aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt tcaaagccca
    14401 tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga
    14461 tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat
    14521 aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga cgctgcgccc
    14581 gttgggccag ttgttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt
    14641 cgcgggtgaa ttgcgaggca tttagcgccc gctctacgtc aacagccgtt ttgccgttga
    14701 tacgcaggcg gatgtcatcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc
    14761 cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc
    14821 cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat
    14881 gttgacggga tcgtccgcta cggcaatcgc cgtattcact aacaccgcgt cggcccccat
    14941 ttccagggcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccggaac
    15001 tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct gattcgagcc
    15061 aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa
    15121 taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc
    15181 ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa
    15241 ttttaaccag tttgtgccta acgcttcacg agccagatgg gcggcgaaaa tggcttcttc
    15301 cgctgttttc gccccggatg tatttggcag cagggtcaca cccgccgcga taagcggttc
    15361 gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg
    15421 gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc
    15481 ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatttcaa ccccctgcaa
    15541 taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac
    15601 gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct
    15661 ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga
    15721 tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc
    15781 cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc tataccgctt
    15841 aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc
    15901 acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag gcagcggtaa
    15961 cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg
    16021 gcggtgataa gcggcgtgtt gagtaccacg caggcggcat taatctcctg gcgagtcgcc
    16081 atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt taacgcctca
    16141 cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc
    16201 tgttggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag gatttgtcgt
    16261 tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg
    16321 cccgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccgataat cagcacctgg
    16381 ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcaggat ttggcggcta
    16441 taacgcataa aatcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt
    16501 tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac tgccgacacc
    16561 cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca cggtgggata
    16621 atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat
    16681 ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag
    16741 cgcgacgtcg atttccatat cgtcatgtgt cgaaacaccc agccgcaggc ctgccgcgcg
    16801 gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc catacgcctg
    16861 atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc
    16921 cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat ctttgatgcg
    16981 tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac
    17041 cactgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat acatcacgct
    17101 tcctccttac gcaggtagat ttctccgcct ctggcacgga agttctccga catatccgcc
    17161 attcccattt caatagtttg cgtggcggcg taatcacgca cttcctggct gattttcatc
    17221 gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttacctga ctcttgcggc
    17281 agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct
    17341 tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga
    17401 tgccctttcg ccaggtcagc ggcgtgggcg gcaatcttat aggtgataag cccctgctta
    17461 acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg
    17521 cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc atagcccggc
    17581 gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac
    17641 tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc
    17701 acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg cagctcggca
    17761 aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc gcccagcgac
    17821 agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa
    17881 ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata
    17941 ccggtcaggc gttttgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg
    18001 aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta
    18061 agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc
    18121 ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag
    18181 agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct
    18241 tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac caggaagtta
    18301 cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcacgt
    18361 ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca
    18421 aagctcattc ccggatgctg gtggcgtaaa acttcgctac gtatgcgctc gcggcccata
    18481 ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc
    18541 agttgggtga cacggcgtcc tgctttggcg cgttttggtg ttagtacgcc gctaaaacgc
    18601 agttcgtcga ggccatcatc tgccagccgc gctttagtgt aatcggaact gcgcacggta
    18661 agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagcccttgc
    18721 tgcacgttaa tggcaatctg cggatcacca tacgggccgg aggtgtcgta gaccggaatc
    18781 gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt cgggctaagc
    18841 tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt
    18901 gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt
    18961 tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag gacgtggctt
    19021 gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata gcagattaac
    19081 tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct
    19141 cagccagtta aggcactccg acaagaaatt agccccgcaa atggggcatt gaatgtaaat
    19201 tacgcgttaa cagcgcagaa ctcaagcagg atgtttgacg cgggcggcgt caagcaccag
    19261 cagaatcagc ttatcttcca gcacaaagcg cgcttccagc gcctcgccga tgtcagataa
    19321 aacctgttga aactcaaggt aattatcatg atcgatggcg gtttccagac tggaatcgta
    19381 gtaatccata atctgttgag tattggcttc gagttgcggc caaatcttcg cggcgcgcgc
    19441 gagttgcccg ttgccttcca gcttatgaag aatgcgctca taaatactga aatgtccggc
    19501 agacaagtaa tcgaccaggc tctgacaaaa atcatcaagg gctttttcgt ttagcctcat
    19561 gtacgattct ttgccaggct taatgccaac cagattgtag taagccacga gcagatgctt
    19621 acgtacatgt agccagcgat caaccagttt gttacttcct ctgacgcgct ccgtcaggtt
    19681 atcgagctgg ttaagcatga ttgactccgc aagtttgtat tcaaaaactg ctcagtgaga
    19741 aatgtaaaaa ccatgttaaa catgccagtg atgcaaaggt agtgcaagag ctatggatcg
    19801 tataattgaa aaattagatc acggctggtg ggtcgtcagc catgaacaaa aattatggtt
    19861 gccgaaggga gaattgccat atggcgaagc ggcaaatttc gatcttgtgg gtcagcgcgc
    19921 actacaaatc ggcgaatggc agggggaacc tgtttggtta gtacaacagc agcggcgtca
    19981 cgatatgggg tcggtacgtc aggtcattga tctcgatgtt gggctgtttc aactggccgg
    20041 acgaggcgta caactggcgg agttttaccg atcgcataaa tactgtggtt actgcgggca
    20101 tgaaatgtat ccgagcaaaa ccgaatgggc gatgctgtgc agccattgcc gtgagcgtta
    20161 ctacccgcaa atcgccccct gcattattgt tgccatccgt cgcgatgatt cgatcctcct
    20221 cgcccagcat acccgccatc gtaacggtgt ccatacagta cttgccggat tcgtcgaagt
    20281 gggcgaaacc ctcgagcagg cagtcgcgcg ggaagtgatg gaagagagcg gaattaaagt
    20341 taaaaacttg cgttacgtga cttctcagcc gtggccgttt cctcagtctt taatgaccgc
    20401 gtttatggcg gaatatgaca gcggcgacat cgtgatcgac ccgaaagaat tgctcgaggc
    20461 gaactggtat cgctatgacg atttgccgtt actcccgccg cccggcaccg tagcgcgccg
    20521 tctgatagaa gatacggtgg cgatgtgtcg ggcagagtat gagtgatgat acactgaccg
    20581 cctgacgcac taaggaacag ccaaaatgac cgaacttaaa aacgatcgtt atctgcgggc
    20641 gctgctgcgc cagcccgttg atgtcactcc agtatggatg atgcgccagg cgggtcgcta
    20701 tctaccggaa tataaagcca cgcgcgccca ggcgggcgat tttatgtcgc tgtgcaaaaa
    20761 cgccgagctg gcgtgcgaag tgactttgca accgctgcgt cgctacccgc tggatgcggc
    20821 gatcctcttt tccgatatcc tcaccgtgcc ggacgcgatg gggttagggc tctattttga
    20881 agccggagaa ggtccgcgtt ttacctcgcc agtcacctgc aaagccgacg tcgataaact
    20941 gccaattccg gacccggaag atgagctggg ttatgtgatg aacgcggtgc gtaccattcg
    21001 tcgcgaactg aaaggcgaag tgccgctgat cggtttttcc ggcagcccgt ggacgctggc
    21061 gacctacatg gtggaaggcg gcagcagcaa agcgttcacc gtgatcaaaa aaatgatgta
    21121 tgccgatccg caggcgctgc acgctctgct cgataaactg gcgaaaagcg tcactttgta
    21181 tctgaatgcg cagattaaag ccggtgctca ggcagtgatg attttcgaca cctggggcgg
    21241 tgtgcttacc gggcgcgatt atcaacagtt ctcgctctat tacatgcata aaattgttga
    21301 tggtttactg cgtgaaaacg acggtcgccg cgtgccagtc acgctgttta ccaaaggcgg
    21361 cggacagtgg ctggaagcga tggcagaaac cggttgcgat gcgctgggcc tcgactggac
    21421 aacggacatc gccgatgcgc gccgccgtgt gggcaataaa gtcgcgttgc agggtaatat
    21481 ggatccgtcg atgctgtacg cgccgcctgc ccgcattgaa gaagaagtag cgactatact
    21541 tgcaggtttc ggtcacggcg aaggtcatgt ctttaacctt ggtcacggca ttcatcagga
    21601 tgtgccgcca gaacatgctg gcgtgtttgt ggaggcagtg catcgcttgt ctgaacaata
    21661 tcaccgctaa ggagtgatta tggatctcgc gtcattacgc gctcaacaaa tcgaactggc
    21721 ttcttctgtg atccgcgagg atcgactcga taaagatcca ccggatctga tcgccggagc
    21781 cgatgtcggg tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata
    21841 tccctcgctt gagctggtgg agtataaagt ggcccgcatc gccaccacca tgccttacat
    21901 tccaggtttt ctttccttcc gcgaatatcc tgcgctgctg gcagcgtggg agatgctgtc
    21961 gcaaaagccg gatttagtgt ttgtcgatgg tcatgggatc tcgcatcctc gccgtcttgg
    22021 cgtcgccagc cattttggtt tgatggtgga tgtgccaacg atcggcgtgg cgaaaaaacg
    22081 gctctgcggt aaattcgaac cgctctccag cgaaccgggc gcgctggccc cactgatgga
    22141 taaaggcgag cagctggcct gggtctggcg cagcaaagcg cgctgtaacc cgttgtttat
    22201 cgctaccggc catcgggtca gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa
    22261 aggctatcgt ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc
    22321 gttcgtgcgc tatacagcaa atcagcccta attcaggtaa actgcggata atttccgtat
    22381 ttgagaactc aacatgttac aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg
    22441 gcagcatgtc actttcatgg cttgcttatg cgaacgcatg taccccaatt acgccatgtt
    22501 ctgccagcaa accggttttg gtgatgggca aatttaccgt cgtattctcg atctcatctg
    22561 ggaaacgctg accgttaaag acgcaaaagt aaatttcgac agccaactgg agaaatttga
    22621 agaagcgatt ccttcagccg acgatttcga tctgtacggc gtttatccgg caatcgatgc
    22681 ctgcgtggcg ttaagtgaac tggtccattc gcgtttgagt ggtgaaacgc tcgaacacgc
    22741 ggtggaagtg agtaagacct ccatcacgac cgttgcgatg ctggaaatga ctcaggctgg
    22801 tcgcgaaatg agcgatgaag agcttaaaga aaacccagct gtagagcaag aatgggacat
    22861 tcagtgggaa atattccgac ttttagctga gtgcgaagaa cgcgatatcg agctgataaa
    22921 aggccttagg gcagacctgc gtgaggcggg tgagagcaat attggtataa tttttcagca
    22981 ataagaccag aaaacgtgat ttaacgcctg atttgtcgta cctggagtct tccctttcgc
    23041 cccccgtctg gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg
    23101 agagtgctat tctggcattt ccgtcgcact cgatgcttag caagcgataa acacattgta
    23161 aggataactt atgaacaaga ctcaactgat tgatgtaatt gcagagaaag cagaactgtc
    23221 caaaacccag gctaaagctg ctctggagtc cactctggct gcaattactg agtctctgaa
    23281 agaaggcgat gctgtacaac tggttggttt cggtaccttc aaagtgaacc accgcgctga
    23341 gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa attgccgcag ctaacgtacc
    23401 ggcatttgtt tctggcaagg cactgaaaga cgcagttaag taagattgcg tggcagtgaa
    23461 cagttttaac gaaggggtgg tttcacccct tttgtctttc tggcgtcgat cattgatgct
    23521 ggcaggcgct ctgtttctca ctgcctgtag tcacaactct tcacttcctc cctttaccgc
    23581 cagtggattt gctgaagacc agggcgcggt acgcatctgg cgaaaagaca gcggcgataa
    23641 tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc gataccacga cgcgagagta
    23701 tcgctggcag ggcgataacc tcacgctcat caatatcaat gtttacagca aaccgccggt
    23761 gaacattcgc gcgcgttttg acgatcgcgg tgatctgagc tttatgcaac gtgaatccga
    23821 tggggaaaag cagcagcttt ctaacgacca aatcgattta taccgttatc gtgctgatca
    23881 gatccgccag attagcgatg ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg
    23941 gcatgcgatg gaacagaccg tgaccacctg cgaagggcaa accattaaac ctgatttaga
    24001 ttcgcaggcg atagcgcata tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt
    24061 ggcatggctg gaagcgcccg aaggttcgca attactgtta gtggcaaact ctgatttctg
    24121 tcgctggcaa cccaacgaga aaacgttctg atttaccagt ggcccatacc catatgaccg
    24181 ccaccaccgc agccgccgta gcccatgccc attccggctc cgcgcggaat acccgcttca
    24241 gccatcgcga tatctcgttt cacccgtaac tcatctaacg actgacgcaa attctccatc
    24301 tctttggcga ccgcgttaat tttgctgcta tccggtgggt tcgcggctaa cagggcattg
    24361 tattcataac gcttcgtcac cagttgctgt tgcagtgcgc tgctttgagc gtaaaagtca
    24421 ttatggattt tctgccacgc tgtctgttgt tcgctggtca aaggcgcggc attttgctgc
    24481 cacataccgt gtccgccgtg agcaaatgca gatgtcgatc ccatcgccat tgctgaaagc
    24541 gccatcatta ccagggcaat tttcgtgttc cgtttcatgg ttaatcctcc agtggttgtc
    24601 ttacttcggg tattgcatct tccgtgccaa cgatgaaacg ctgatatgac gggtaatctg
    24661 gcatgataaa cgagtaaaaa tgactcgcct gctgcgggta gcgagtcatt tttactcatt
    24721 gaaacttaag cctttgtgtt acagcgcagg gtaagcgctg ataaaagatg gcatgatttc
    24781 tgctgtcaga aagggatgag caggcaaaga agaagatgcg ttttatgcaa cgttctaaag
    24841 actccttagc taaatggtta agcgcgatcc tccccgtggt cattgttggg ctggtagggt
    24901 tgtttgcggt gacggtgatt cgcgattatg ggcgcgagac tgccgccgcc agacaaacgc
    24961 tgctggaaaa aggcagtgta cttattcgcg ctcttgaatc cggctcgcgc gtcggcatgg
    25021 ggatgcgcat gcatcatgcg cagcagcagg ccttactgga agaaatggcc gggcagcctg
    25081 gagtacgttg gtttgcggtc acggatgaac aaggaacaat cgtgatgcat agcaactccg
    25141 gcatggtggg aaaacagctt tattccccgc aggaaatgca gcagttacat cctggagatg
    25201 aagaagcgtg gcggcggatc gatagcgcag acggcgagcc tgttctggaa atttatcgcc
    25261 agtttcaacc gatgtttgct gctggaatgc accggatgcg ccatatgcag cagtatgccg
    25321 caacaccaca agcaattttc atcgctttcg acgccagtaa tattgtgagt gccgaagatc
    25381 gtgagcagag aaacaccctg attatcctct tcgccctggc gacggtcttg ctggcaagcg
    25441 tattgtcatt cttctggtat cgccgctatc tgcgctcgcg ccagcttcta caagatgaaa
    25501 tgaagcgcaa agagaagctg gtggcgctgg ggcatcttgc ggcaggcgtt gcccacgaaa
    25561 tccgtaaccc actttcctcg attaaaggac tggcgaaata ctttgccgaa cgcgcgccag
    25621 cagggggaga agcgcatcaa ttggcgcagg tgatggcgaa agaagccgac cgtttaaacc
    25681 gcgtggtaag cgagttgctg gaactggtta agccaacgca tctggctttg caggcggtgg
    25741 atctcaacac gctgattaac cactcattac agctggtaag ccaggatgca aacagccggg
    25801 agatccagtt acgctttacc gccaacgaca cattaccgga aattcaggcc gatccggaca
    25861 ggctgactca ggtcctgttg aatctctatc tcaatgctat tcaggcgatt ggtcagcatg
    25921 gcgtgattag cgtgacggtc agcgaaagcg gcgcgggcgt gaaaatcagc gttaccgaca
    25981 gcggtaaggg aattgcggca gatcagcttg aagccatctt cactccgtac ttcaccacca
    26041 aagccgaagg caccggattg gggctggcgg tcgtgcataa tattgttgaa caacacggtg
    26101 gtacaattca ggtcgcaagc caggagggaa aaggctcaac gttcaccctc tggcttccgg
    26161 tcaatattac gcgtaaggac ccacaaggat gacgcacgat aatatcgata ttctggtggt
    26221 ggatgatgac attagccact gcactatttt gcaggcttta ctgcgcggct ggggctataa
    26281 cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag gtgcgggaac aggtttttga
    26341 tcttgtgctt tgcgatgtgc gaatggcgga gatggacggc atcgccacgc tgaaagagat
    26401 caaagcgtta aacccggcga ttccggtact gattatgacc gcatactcca gcgtcgagac
    26461 ggcggtagag gcactgaaaa ccggggcgct ggattatctc atcaagccgc tggatttcga
    26521 caccctgcag gcgacgctgg aaaaggcgct cgcgcatacg cacagtattg atgctgaaac
    26581 gcctgcggtg actgccagcc agttcggtat ggtcggtaaa agcccggcga tgcaacacct
    26641 gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg gtgctgatcc acggcgattc
    26701 cggcaccggt aaagagctgg tcgccagggc gattcacgcc agtagcgcac gtagcgaaaa
    26761 accgctggta acgctcaact gtgcggcact caacgaatcc ttgctggaat ctgaattgtt
    26821 cggtcacgaa aaaggggcgt ttactggagc cgataaacgc cgggaagggc gctttgttga
    26881 ggcggacggc ggcacgctgt ttctcgatga aattggcgat atctcgccga tgatgcaggt
    26941 gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt gtcggtagca accagactat
    27001 ctcggttgat gtccggctga ttgcggcgac ccatcgcgat cttgccgcag aggtgaatgc
    27061 cgggcgtttt cgccaggatc tctactatcg cctgaatgtg gtggcgattg aagtaccatc
    27121 gctgcggcaa cggcgggagg atatccctct gctggctagc cattttcttc agcgttttgc
    27181 cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag gcgatggatt tgctgatcca
    27241 ctacgactgg ccgggaaaca ttcgtgaact ggaaaacgcg gtggaaaggt cggtggtgct
    27301 gctgaccggg gaatatattt ccgaacgcga gctgccgctg gcgattgcca gtacgccgat
    27361 cccgctggga caaagtcagg atattcagcc gttggtggaa gtggaaaaag aggtgattct
    27421 ggcggcgctg gagaaaacgg gcggcaacaa aaccgaagcc gcccgtcagt tagggatcac
    27481 gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc tcgcgttcga tagcgcgcca
    27541 gccgatatct ttccggcaga agcagtcgtc ccagtgaata tcggtcatta aggcataagc
    27601 gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg acgcacagta cgcgcccgcc
    27661 gctggtcact acctgctcgt catccgccag ttttgtgcct gcatggaaca ctttgccgtc
    27721 ttccacttct tccagcggca ggccgtggat cacatcaccg gtgcggtaat cgcccggata
    27781 tccacccgca gccatcacca cgccgagaga agcgcgttca tcccactcgg atgttttctc
    27841 gtccagcttc ccttcacagg ctgccaggca gagctcaacc agatcggact tcatgcgcag
    27901 cataatcggc tgggtttccg gatcgccaaa gcggcagtta aattcgataa ccttcggatt
    27961 gccctgtttg tcgatcatca ggcccgcgta gagaaaaccg gtgtaggtgt tgccttccgc
    28021 cgccatgcct ttcacggttg gccagatgat acgttccatg gtgcgctgat gaacttcatc
    28081 ggttactacc ggcgcggggg agtaagcgcc catcccgccg gtgtttggtc cggtatcttt
    28141 atcgcctacg cgtttgtgat cctggctggt agccatcggc agcacatgct cgccgtccac
    28201 catcacgata aagctcgctt cttcgccatc gaggaactct tcgataacga tgcgatgacc
    28261 cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca gccgcttccg cttcttccag
    28321 cgtcatcgcc acgataacgc ctttcccggc agccagaccg tccgctttaa tgacgattgg
    28381 cgcgcctttc tcacgcagat acgccagcgc aggttctacc tcggtgaagt tctggtattc
    28441 cgccgtaggg atcttatggc gggccaggaa atctttggta aacgcttttg agccttccag
    28501 ttgggccgca cctgcggttg ggccgaagat tttcagcccg gcggcgcgga aggtatcgac
    28561 cacgcctttc accagcggcg cttccgggcc gacgatggtc agatcaatct tttcgttttg
    28621 tgcgaaatcc aacagcgccg ggatatcggt tacgccgata gcgacgttct gtagcgcggg
    28681 ttccagcgca gtgcctgcat tacccggagc aacaaaaaca gtttcaacca gcggcgactg
    28741 cgccgctttc caggccagcg cgtgctcgcg cccgccgtta ccaatcacta atactttcat
    28801 ctattgctcc attaatggcg gaagtggcgc atgtcggtga agagcatcgc aataccgtgc
    28861 tcgtcggcgg cggcaatcac ttcgtcatca cggatagaac cgccaggctg gattacgcag
    28921 gtcacgcctg cagcggcggc ggcatcaata ccgtcgcgga acgggaagaa cgcgtcagaa
    28981 gccatcgagg aacctttcac ttccaggcct tcatcggcag ctttaatacc ggcgattttc
    29041 gcggagtaca cgcggctcat ctggcccgcg ccaatcccga tggtcatatt gtttttggca
    29101 tagacgatag cgttggattt cacgaacttc gccactttcc agcagaacag cgcatcacgc
    29161 agttcctgtt cggtcggctg acgttgggtg acgacgcgca gttcttccgc accgaccatg
    29221 cccaggtcac gatcctgaac cagcagaccg ccgttcacgc gtttgaaatc aagacccgga
    29281 acacgctcgc cccactgacc gcaggtcaga acgcgtacgt tctgcttggc ggcggtgatt
    29341 ttcagggctt cttcgctggc ggaaggcgca ataatcactt cgacaaactg acgagaaatg
    29401 atggcctgtg cggtttccgc atccagctcg cggttaaagg caatgatgcc gccgaatgcg
    29461 gaggttgggt cggttttgta cgcgcgatcg taagcatcaa gaatagaatt gctgatagcc
    29521 acgccgcaag ggttggcgtg cttcacaatc acacatgccg gctcggcgaa ctctttcacg
    29581 cactccagcg ccgcatcggt atcggcgatg ttgttataag agagggcttt accctgaacc
    29641 tgggttgcgg tagcaacgga ggcttctttc acattctctt ctatatagaa ggcagcctgc
    29701 tggtggctgt tctcgccgta acgcatatcc tgcttcttaa tgaagttcag gttcagcgtg
    29761 cgtgggaagc gaccggcggc ttctttgctt tcaccgtggt aagccggaac catgctgccg
    29821 aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt cgaaggcttt gatggcgagg
    29881 tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat ccatctcttt aataatggcg
    29941 tcgtagtcgc tgctcttcac cacgattgcg acatctttat ggttcttggc ggcggagcgc
    30001 accatcgttg ggccgccgat atcgatgttc tcaaccgcat cttccagcga gcaaccttca
    30061 cgggccacgg tctgggcgaa cggatacagg ttaacaacca ccatatcgat aggctggatc
    30121 tgatgttctt ccataatggc atcgtcctgg ccgcgacggc ccagaatgcc accatgtact
    30181 ttcggatgca gggtcttcac gcgtccatcc atcatctccg ggaaaccggt gtaatcggaa
    30241 acttcggtta ccggcagacc tttttctgct aacagacggg cagtgccccc tgtagacagc
    30301 agctccacac cgcgtgcgga aagtgcctgg gcgaattcga cgataccggc tttgtcagaa
    30361 acactgagca gagcgcggcg gactggacga cgttgttgca tggtaaatcc cctggatttg
    30421 actattacag agagcgttag ctgaattttt cgcgaaaaac tcagctaacg cccctaacgg
    30481 ggcatcctta tttttcgccc gcattgtaac gaaaacgttt gcgcaacgct cgcgaatttt
    30541 tctctttcaa tggtgatcac aattttgaca gtggttaccg tgggaaaaat acagaaatta
    30601 cattgatgat tgtggataac tctgtgcgta aaaaggtata aagcggggtt ttgctgggga
    30661 atgcagcagt cagtcatttt tctgcaattt ttctattgcg gcctgcggag aactccctat
    30721 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    30781 aagagaaaaa tcctgaaatt cagggttgac tctgaaagag gaaagcgtaa tatacgccac
    30841 ctcgcgacag tgcgctaaag
//
LOCUS       DAPGWD010000040        30590 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPGWD010000040 DAPGWD010000000
VERSION     DAPGWD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.040"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(393..683)
                     /locus_tag="RHX88_001783"
     CDS             complement(393..683)
                     /locus_tag="RHX88_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2230478.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(684..995)
                     /locus_tag="RHX88_001784"
     CDS             complement(684..995)
                     /locus_tag="RHX88_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2230479.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1224..2132
                     /locus_tag="RHX88_001785"
     CDS             1224..2132
                     /locus_tag="RHX88_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2230480.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2196..3137)
                     /gene="fabY"
                     /locus_tag="RHX88_001786"
                     /gene_synonym="yiiD"
     CDS             complement(2196..3137)
                     /gene="fabY"
                     /locus_tag="RHX88_001786"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDV2230481.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3182..3619)
                     /gene="dtd"
                     /locus_tag="RHX88_001787"
     CDS             complement(3182..3619)
                     /gene="dtd"
                     /locus_tag="RHX88_001787"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDV2230482.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3616..4488)
                     /locus_tag="RHX88_001788"
     CDS             complement(3616..4488)
                     /locus_tag="RHX88_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDV2230483.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4482..5081)
                     /gene="yihX"
                     /locus_tag="RHX88_001789"
     CDS             complement(4482..5081)
                     /gene="yihX"
                     /locus_tag="RHX88_001789"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDV2230484.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5222..6109)
                     /locus_tag="RHX88_001790"
     CDS             complement(5222..6109)
                     /locus_tag="RHX88_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV2230485.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(6099..7448)
                     /locus_tag="RHX88_001791"
     CDS             complement(6099..7448)
                     /locus_tag="RHX88_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV2230486.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7547..8980)
                     /locus_tag="RHX88_001792"
     CDS             complement(7547..8980)
                     /locus_tag="RHX88_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2230487.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9050..10060)
                     /locus_tag="RHX88_001793"
     CDS             complement(9050..10060)
                     /locus_tag="RHX88_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDV2230488.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10093..10980)
                     /locus_tag="RHX88_001794"
     CDS             complement(10093..10980)
                     /locus_tag="RHX88_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV2230489.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(11005..11883)
                     /gene="yihU"
                     /locus_tag="RHX88_001795"
     CDS             complement(11005..11883)
                     /gene="yihU"
                     /locus_tag="RHX88_001795"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDV2230490.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12056..12952
                     /locus_tag="RHX88_001796"
     CDS             12056..12952
                     /locus_tag="RHX88_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV2230491.1"
                     /translation="MTKIACVGITVQDRIYSLLTLPDGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLIALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            12992..13780
                     /locus_tag="RHX88_001797"
     CDS             12992..13780
                     /locus_tag="RHX88_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2230492.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(13891..15156)
                     /locus_tag="RHX88_001798"
     CDS             complement(13891..15156)
                     /locus_tag="RHX88_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2230493.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(15258..16238)
                     /locus_tag="RHX88_001799"
     CDS             complement(15258..16238)
                     /locus_tag="RHX88_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDV2230494.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(16246..16956)
                     /locus_tag="RHX88_001800"
     CDS             complement(16246..16956)
                     /locus_tag="RHX88_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDV2230495.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(17173..18996)
                     /gene="typA"
                     /locus_tag="RHX88_001801"
     CDS             complement(17173..18996)
                     /gene="typA"
                     /locus_tag="RHX88_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDV2230496.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            19369..20778
                     /gene="glnA"
                     /locus_tag="RHX88_001802"
     CDS             19369..20778
                     /gene="glnA"
                     /locus_tag="RHX88_001802"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDV2230497.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            21097..22146
                     /gene="glnL"
                     /locus_tag="RHX88_001803"
     CDS             21097..22146
                     /gene="glnL"
                     /locus_tag="RHX88_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDV2230498.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            22158..23567
                     /gene="glnG"
                     /locus_tag="RHX88_001804"
     CDS             22158..23567
                     /gene="glnG"
                     /locus_tag="RHX88_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDV2230499.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            23679..23789
                     /locus_tag="RHX88_001805"
     CDS             23679..23789
                     /locus_tag="RHX88_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDV2230500.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(23979..25352)
                     /gene="hemN"
                     /locus_tag="RHX88_001806"
     CDS             complement(23979..25352)
                     /gene="hemN"
                     /locus_tag="RHX88_001806"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDV2230501.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(25541..26050)
                     /gene="yihI"
                     /locus_tag="RHX88_001807"
     CDS             complement(25541..26050)
                     /gene="yihI"
                     /locus_tag="RHX88_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDV2230502.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            26632..27264
                     /locus_tag="RHX88_001808"
     CDS             26632..27264
                     /locus_tag="RHX88_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDV2230503.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(27646..30432)
                     /gene="polA"
                     /locus_tag="RHX88_001809"
     CDS             complement(27646..30432)
                     /gene="polA"
                     /locus_tag="RHX88_001809"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDV2230504.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         7681 a         7967 c         7401 g         7541 t
ORIGIN      
        1 ggatgtttat tttttgattg gatctttatt tgcataatct gaatggtgta cccgaaaaat
       61 gtcagcatcc taacagcacc cggatttaag gtgaaattaa tcatccatca cagaccggtc
      121 agtactgcca ccaaccggtc tttacttgtt catcgttata attttgttgg taacagttcg
      181 ggaaacaagg aattccttcc ggaacccccc ggtatcatcc gatagcattg acgtcaatca
      241 tcagcgcgac gatgagtttt tttaatcgca tcatagggtg gcgacgttca gcacgattac
      301 gggtcatagc aaaactcctg ggcaggcgac cactatgccg ctttcacagg agttctgaat
      361 gctagtgctt tctgtatggc ataaccatgc ctctaacggt taagtgcttg cagcacatgt
      421 tgcggatcat tggcaatcgc tcgcagcaaa gcctttgcag gcccggtcgg tgaacgtcgc
      481 ccctgctccc agttacgcaa cgtgtctacg cttacagaaa taagatcggc aaatttagat
      541 tgtgacaacc ctgaagcgcg gcgtatttct ttgattttca tagcatcaac ttcaaaagtc
      601 cgcgaaggct gtcgcgttcc ttcaataatt tcattcatct gggccatgct gtgagtcaac
      661 cgctcaaaaa gtgccttatc catttaccac ctcgtattta acaaccggag cattgctttt
      721 tcctggggag acagatcatc tttaataccc ttacgataaa taagcagtaa tctgatctca
      781 taatgtttaa cctgatgaaa gtaaataacc ctgacaccag cacgtttccc ctttcctcct
      841 gaaacccatc gaacctttct taacccacca gtttcaggta ttacctcacc atagtcagga
      901 ttcaaggcca ggaaaaactg aaaacgactg aattcatcgt cggtcagcag tttttgaaca
      961 tcttcagtaa aaatttccgt ttcgatgaag agcataatta tacgccattg gcttagagta
     1021 acaatccttt ttgtacgtca ttgacgtacc cttgaaacca ctagcttact gatagtgctc
     1081 cagcaaaacc acgtctctgc cgggtttgtc tttagatttg cgaggcgctt tccagatttt
     1141 tcacgcccgt gaatttccct gtatccatcc agcaaaaacg ggtgtataag actgtacatt
     1201 catacagcaa aataagttct actatggcac tggaaaaagg aattgcagag ttagtcgaaa
     1261 agtttatcgc tgcgggtcgc ccctcatcgc gtcaacaaag tattgcgcag cgcagggagg
     1321 gctatatcgc cagcgctgtc cttgcgggtg agacagaaac gcgggtagat attcagacta
     1381 tcgaacttga agggatgacc ttacgaatag tgtcgccgct gaatgccccg acattactcc
     1441 cgacgataat ttattactac ggtggctgtt ttgttagcgg cgggttcgca actcacgaca
     1501 accagctgcg ccagttagcc tattacgggc aatgtcgggt tattgccgtc cagtatcggc
     1561 tggcaccgga acatactttc cccgccgccc atgatgatgc gcaacgaggt gcagaaatag
     1621 tctggcaaca cgccgaacga ctcggcgttg ataaacaacg tataactctt gccggtgaca
     1681 gtgcgggcgg acatctggcg ctggtaactg ctttgcgatt aaagcgtgct ggcgagtggc
     1741 aaccggcaca actcatctta atttatccta tgcttgatgc gactgcgcat ttcgaaagct
     1801 acatacgcaa tggtcacgat tacatcatca cacgcgacac tttactgagc ggctttgaga
     1861 tgtatctgcc aggcattgaa cgcaggcatc ctgaagccag ccctctttgg cgcgatgatt
     1921 tcaatggctt gccacctgta catatcatta ccgctgaata cgacccactt tgcgatgaag
     1981 gtgaagcgtt gtatcaccgc atgacagggc aaggagtgca atgcacctgc caacgctacc
     2041 ttggcgttat ccacggattt tttcagttag gtggtatcag cgaggccgcg cgatcggcac
     2101 tacgggatgt tgcctggaga gcgggaaggt aacttcggac ctgggccctg aaaaggtttt
     2161 cagattgaaa accttttcag tcaagtaaaa cgcccctact cttcttcgtt cccgccctct
     2221 tcatacgggc caaatggctt cgcgggcaga acgatatacg tgccttcaaa cactgcaccc
     2281 ggcgtctcgt cgccaaagat ttcaacttgc atctgcaccc gtgcttttcg tccgcgcgcc
     2341 agacggtcga gatcgccgct taaggcacca aggtcggcta ccgcatgagg tttaccgcta
     2401 atcggcttgc tgtagcggat atgcgcatcc gccagaataa tcgttccgcc gagatggcgt
     2461 tcgcgcagca tcagccagat aagcccccaa ccggtgagcg tcgccagcga gaataaactc
     2521 ccggcaaaca gcgtatggtg cggattctga ttgccggttt ccggcatggt ggtgataaat
     2581 ttttgcccgg tatattgttg aatacgcacg cccatttttt cactaagcgg gatatgttcg
     2641 taccacgcct gttgcagctg cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg
     2701 gcgacgggct taatcatcaa aaaatggcga atcggcgtgg tggttggcgt ggtgatttct
     2761 ccctgattaa caaaccccag cttggcgaaa aactccaccg cgtcttcacg ggcgctacag
     2821 gtcacgcgct taacgccttc ctgacgcgcc accgactcca gggtcatcgc catcagcgtt
     2881 cctaaccctt tgtcctgcac gtccgggtga acggccataa agcgaatgga cgcttcattg
     2941 tcggcattaa tatacagtcg gcctaccgcc accagattac cctgctcgtc gacgaccatc
     3001 tggtgatgcg ccatcgcatc ccacgcgtcg cgttccgaac cttttggttg atgcaggggc
     3061 ttacgcaaca tttcccagcg aaactgatag taacgctcta attcttcttc tgtttgtgga
     3121 acccgaaggt gatacatagc gatactctct cttgtttccc gtgacaaccc tggaagctgg
     3181 ctcatacctg caaccagaat gtcacggggc catcattgac cagcgatacc tgcatatcgg
     3241 cagcgaagcg tcctgtttgc gtgttcatct cttgctgacg gcagcgttcg acgaaatagt
     3301 catataacgc ctctgcgcga tccggtgatg cacctttgga gaaacttggg cgcatccccc
     3361 gttcggtatc tgcggcgagg gtaaactggg aaaccaccag cacactgccg cccgcctgtt
     3421 gcacgttgag gttcatcttg ccttcggcat cgctaaagat gcggtagccg agcacacgct
     3481 cgcacagacg gtttgctttc tgttcgtcgt catccttttc gacacccaat aacaccaaaa
     3541 gtcccgcgcc aatttcgccc gtcacttctc cctccacggt gacgctggca cgggttacgc
     3601 gttgaattaa tgcaatcatg gttcgtcgtc ttcttcttgt tcagctgctt gttttagttt
     3661 gcggtattcc ccgagagtga cagtaatttc cgcgccaagc aagacgatac accatgtcca
     3721 gtagacccaa acaaagagaa tggggatcac cgccaacacg ccgtaaatga gctgatatga
     3781 cggaaacatg gtgatataaa gcgcgaaacc tttctttcct gcttcgaaca ggagcgcggc
     3841 gacaaacgcg ccgacaatcg cgtcgcggtt aggtacgcgg atggtaggaa caatgctgta
     3901 cagcaaccag aaggagatcc acgacaacag cagcggaaaa atacgcagca cgttatcgat
     3961 gacagtattg agatcgctcg cccagcgcag ggagagcaaa taggaactga tcgccagact
     4021 ggcccctgcc agcagcggcc ccagcgttaa aatcatccag tacacggcga acgagtaaat
     4081 ttttggtcgc gctcgtttac tgcgccagat ggtattcaac gcgctatcga tggagtacat
     4141 cagcaataac gcggtgacga tcagcccgca cgccccaacg gcggtcatct tgttggaatt
     4201 ggcaacaaat tgttcgatat accgctgaat aacatcgcca gtagcaggca gaaagttggc
     4261 aaaaataaag tgacgtaact gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag
     4321 cgcaaaaaca acggcaacca gcggcactaa tgagagcaac gacacatagg caaggttacc
     4381 tgccagggtt gtcatgttgt cctcatcaat gcgttgccag agtagtttta gccaggccca
     4441 tagtggacgg gtacggtgcc tggctttgtc ctgaatggtt tttagcataa caccttcgcg
     4501 aaatagtccg ggatggtggt tttatctttc accagaatac tggtaatgcc cagctgattg
     4561 gctccttcta tattatcggc gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct
     4621 tccgcctgca aaacatgctg gtaaattcgt gcttcaggtt tgcgcatccc cagatcttgc
     4681 gacagataga tatggtcagc agcatcacga atttccgggt attcttccgg ccagaaggtg
     4741 gtatgcaggc ggttggtatt ggaaagcacc accacgcgat gcccctgctc acgcagttta
     4801 tgcatgatgg cgatcacttc cgggcgcagc gcaacaaaca ccgcctgcca gccgtgagag
     4861 aactgctcgt agcttagcgg tagagccatc tcatgacaca gcgcctctgc gaacgcttcg
     4921 tcgctaattt ccccacgctc atgctgatga aacgcctccc ccatatgaaa actcttctta
     4981 agcgatgcca gcggaatacg cgttaaatcg ctccaggctc ccagcacacg gttaaagtcg
     5041 atatcgacaa tcacattacc taaatcaaag atatagagca tttttgcctc ctttcgcgcc
     5101 acaagaaatt aactgtagcg ggaaaggatg ggtttggcta tgtggcaata agggtaatct
     5161 tgtgatgctg attgcgaaaa agcagagtaa tttcactggc tttttcgcaa tagatgaatg
     5221 gttaagattc aggtgtaaat tgccaacgag tttcgatgac cagcggccta tctggtgtca
     5281 ccaattccgg cgttagattt attgcgttag gtgcctgagt cagcgggtta atacaggtgg
     5341 catcgggctg tttatcaaat accaccagac tgtttgcaga agagatcatg gtcatcgcga
     5401 gtttaccagg ccacagtaac ttaaccttca ccccgtcata aaaaccaaaa cagtcatccc
     5461 agggaccggc ttgaggagag atacgatttc cagttggcag ttcatcacta cccgcttctt
     5521 cttgccagtc cgcatcaaaa agcacctgca acgattctgt attttgcgga gtcagctttt
     5581 tcgcaaacca cggatgccag cctgcggagg ccgggaatgt atcggcatat gaatggactt
     5641 ccagttgcaa aaccagcgca tcgttctcca gcaaaaaagt ctgaatcact tccccttgcc
     5701 agggccacgg tgaagccagg ggcatacgta aagtcaggga atctgctgtt ttgtcgatga
     5761 tttcccacgt tgagtaacaa gccataccat gcaacgcatg tggtggttta ttggcgggta
     5821 aagaataaca ttgcccacca gcattcagcg tcgcattccc cagtcgccct gcccacggca
     5881 ccattgggaa acagccatac tgaaaagctc tgcgctgcgg ctgccattgg cgtaatactt
     5941 cagaaccaaa tgcctttaac gaggttatcc tggcaccatc atgcggataa atctgcaggt
     6001 gaatattgcc tttttccagc gtcactaact ctcctacagg cccaggccat ggcgcgtcaa
     6061 acgcctgttg tagcgccagg taatgctcgt tttttcgttc agttttcatg agattgttcc
     6121 ctgatgtagc ggtaagaagc ggtttcttgt tcatcggtcg gctcaatcag ccagacgcaa
     6181 acaaaggaaa gaactgagac agcaaataaa aacagagcga cataccacgg ttttcctccc
     6241 cctagcgcca gcaaacttga ggcgataagg gggcttagtc cgccaagaat cgcggaaaat
     6301 tgataagcaa aagaaaggcc ggtatatcgt actttggtac cgaacatgcg ggtgaaaagt
     6361 gttggctgca cggcaaacat catcgtcgga cctaagttgt agccgaggat catactgcac
     6421 cagatgatga gcgtactttt gctatccagt aataagaaga aaggaaacgc gaacaggaca
     6481 caaaatcctg ctcccgacaa atacagactt ttttgaccta tacgatctga taaccatcca
     6541 accaatggac aactgaaaat accgatcgcc gaggcaatca acatacccgt caggggaata
     6601 tctcgcgaca atgctaattg gctggaaata tagacaatac caaaggcgtt aataatatta
     6661 gaggagacac tttccgccag cctcgcaccc agtgccaata aaatatttcg tggatgtttt
     6721 ttgaataatt caatcaacgg aggaacggac ttttctttat tattcgtcgt tttttgcttt
     6781 tgaaaatcca gcgtttcttc ggtatgcagg cggataaaca gcccaacgat caacatcacc
     6841 gcagaaagcc aaaacggaat gcgccatccc caggagagaa actgctccga agtaagaaaa
     6901 tggttacaga gagcaaaaat accggttgcc agcataatac ccacagacgc agccgtttgc
     6961 gggagtgaac ctaaaaatcc gcgtcgggat tcgggcgctg attctatggt catcaatgcc
     7021 gctccgccat actcgccacc aagaccaaag ccctgaatta aacgcagcgc cattaatatg
     7081 agcggtgccc agatcccaat ttcctgatac gtcggaataa aaccaatcaa aaatgtggag
     7141 caaccgacaa tagccagtgt ccagataagt gttatttttc gcccaatttt atcgccaaaa
     7201 tggccaaaga cgataccacc taaaggacgg gtcaaaaaac caacgccaaa ggtagcaaag
     7261 gcggctataa gacctataaa aggatcgctg tctggataaa acagcggtgc aaataccagt
     7321 cctgctgccg tgccaaaaat aaagaagtca taccattcca ttaacgcgcc tgcgaaactg
     7381 cccataacga cacgtctcat ggctttaccg gaaagccctt cacgcggtgc gggtaacttt
     7441 tcagacataa ttttcctctc ctggtaggcg agaaatctgg gatacgaacc actaaacgtg
     7501 ggcagtttga attaatgcgc atcggtttaa gccgatgcgc attaatttat ttcttcaatt
     7561 cagccagaac ctctgcatcc tcagcatcaa catttgcacc cagtggttcc caattaatca
     7621 atacgcaggt caggaacccc aagaatggca cgattgccaa ccagtaaaac gcttcagttt
     7681 tcaacgcccc ccacagcatc ggccagaaaa tgagtcctgc aattgcccct gtgcgcataa
     7741 tagcgcgggc aaatccgacc ccagtagggc gaatagcggg aggataagag agtgtggcaa
     7801 tggtcatgcc cagcccacca ggacccgctg aatggaagaa aataattgat cccaataacc
     7861 ctaaagacag ccatggatta gtcgttgcca gtgcgcctaa cgacagcaat gctaaggtca
     7921 caacagcaaa tccatacatc gactgtcggc gagtacccag tcgttggaga ataaacgagc
     7981 cgatcatccc gcctgtcacc ccacaaaggg cattcacgac ggcagaaccc gtcaacgcac
     8041 cagtaagccc gccacttatt attcccgcca acgtaagagg aagataaaca cctacggcgt
     8101 tatattgcca cgcctgcatg gttgccacga cacagccaag aatggtgcgt ttgcggtaac
     8161 gatcattaaa taagatgcca tatccaccac tgtatttatt ctctgctttt tccgagaatt
     8221 tcgcttctgt tgtactcgat gccgcaacgc gagcctgaac accataacgt tttcccagaa
     8281 tgtcgcacgc ttcctggtaa cgcccgacgc gtgcggccca cattgcggat tcaccaataa
     8341 agaagtaacg caaaatcatg aagatgactg caaaaatagc tccgatgaat attccgtaac
     8401 gccacaattg ttcttcagcg atacccgaca gaagcagcgg caagagcacc aaataaacaa
     8461 cagttgtcga aacataccag gccatttgcc agaggttgac cgacgttccg gttttcttcg
     8521 acgttgaacc acgaagctct gccacggcat tggttgccaa agggaagtcg atgcctaaac
     8581 caaagcccat gatgacgcgg cacaccagca cccaccagat actgggcgca atagctaccg
     8641 cagcagcccc gatggtgcac agccccatgc caataataaa tgctcgcttt tgcccaaagc
     8701 ggtttattac tcggttcgct aataaaccgc caattaacgc ggcgattaat actgacgcat
     8761 taacggttgc ggcaaggcct ggagaaatgc caaattgagc ggcaatgtat ttattaccaa
     8821 accccaccat cgccgcctgg taggcatcaa tcaatatgcc gcccaaagca ataaaaataa
     8881 cgccgatact ggtgttgatt gcagagttct tattaacaat atcaattacg tcctgtggcc
     8941 gactaattga ataagttgca ggactggtga tttgactcat cgcagtgtcc tttttaattg
     9001 ttgtagggtg gcgctgacgt gacgcagcgc cagacagaga tttagaaaat cattccggcg
     9061 caaattgcag catgaccttt atggagtcat tttgctgcgc gcgttcgaat gccgccgctg
     9121 catcctcaat ggcaaataaa tcggtaacca gttcactggt atcaaaccgc ccatccgcca
     9181 gccattcgat aacagcatcg aaatcttcag ggatgtacat gccagagttg agcaagtcac
     9241 gctcgaaacg ttgcatcatc ggtaatggta tttcacgcgg cccacttggt acgcccatgc
     9301 acacgacggt gcctccggca tatacacggg tacatgcatc gttaagcgtg gcctgcgctg
     9361 caacgacatc aatcgccccc gtaaatcgca catctgcggc cagctcacct ggtgcccaaa
     9421 cttctgctgc gcccagcttt aacgccagcg cgcgtttggc agcgtcaggt tcaatcacgg
     9481 taatcttacc tgcccccata atgcgcagtg cctgcacgat ggataagcct atcgttccgg
     9541 caccaatcac cagtacgctt tccagagaag ctttcggcat tcggttaacg cagtgacggg
     9601 cacaagccgc cggttcggca aaggccaaca cttttagcgg taaagaggct ggtgcgacat
     9661 ggcaattacg cgcaggaaca atgtgttgtg agcgagcaaa gcccggtgcg cgaaaaccag
     9721 caacctgcgg tggttcacaa agattaaaac gtcctgcttt gcaggctcgg cattccatgc
     9781 aagccatgat gggatcgacc acaacatgat cgcccggctg tacattttta acgtcgctgc
     9841 caacttccgt aacgcgcgct gcaatttcat gaccggggac gacaggtggt ttagcaaacg
     9901 gatgaccgcc tttcagaaca tgcagatccg aaccgcatat cccatagaac acaggcgcaa
     9961 tccgcacttc gtgcggttta agttcctggt gtggtacttc aacatattga gtggtgactt
    10021 tcccaatatc tgaaaataaa acctgggtaa ttttatccat tgttattctc cagaatcgat
    10081 atccataggg gattagcgtt gcgcgatacc ttcatcgaca atttctgcca accgttgtaa
    10141 acgaggaaca gagacatcgc gcagcatagt ttgtggatct tgtgcaccaa caaccgacgc
    10201 ccaaaccgct cgtccagcca ggaagcctga cgcaccacct ttcatggcaa tacttactgc
    10261 acggccaaaa atatcggcat caacgccaga agaaagaatt acccacggca tcttcatctg
    10321 atcgttaagt tgctgacagg cggcaagtaa ggttttttca tcgcctttgc cccctaatgg
    10381 catctcggct ttgtaaagat cagcctctgt tccaccaagc tctgcggcgg cggcaacaat
    10441 ggcgctttca cgatcaaagt cccaaccgcg acgaggtgga cgcaccacag gttcaataat
    10501 gctaaccagc ccggcactac ggcagcgacg aacaaattta tcaaccattg ccagacgttc
    10561 ctctgcgggt tcatcttcac gccagagtac aagtaatttc attgccttag cccccatttc
    10621 acgggcttta tgcgggtcga cggacatatc tatttcaacg ctatcaaccg ggatgccatt
    10681 acctgggata aaaagatcgg cagcgacaat caggccgcaa gaattagcaa cagcgccctg
    10741 ctcgactatt tgctcaagac aaaattgttt atccgccaga acggcagaag cataaggaga
    10801 aaggattcgg gtcgcggcaa ctttaaaatc ggtgagtaca ctgtcggcga tgggttttgg
    10861 ctgccctgct gccgcaaaca tcaagcgcat ggcctcacgc tgatcgacag ctaacatggc
    10921 aaatccacca cctgggcggg agatatcttt cagcgtaaat ggggtgaagt gggatgacat
    10981 agaaactcct gttaatagat aaaattaaag accagcctgc tggcgaacct gatttagcag
    11041 ggcactccag tcctgccgcc ctttgccgtc ttgacttgct gcagcgtaat aactttctgc
    11101 tgcaatcccc atattgagcg gtgcccccgt ctcccgagcg acgtcaactg caatacgcag
    11161 atctttcaac gccagatcca ccatgaaggc gggtgaaaga tcgcctttca gcactttgca
    11221 aggccaggtg gtcgtaaaat gtcctttccc tgctgccgta ccactcatca ccttaattgc
    11281 gacatcaaga tttaatccca gtgactcaca taacacagcg gcctcggagg acagcgcatt
    11341 aagcgcaatg ctcatatagt tgttaattag cttcacgcgg atccccattc ctgggccgcc
    11401 agcctcgacc agctcattac ccatgcacat gagaatcggg cgtgcacgtg caatctgctc
    11461 atcagtgcca cccgctaaaa tcagcaatgt gccatcaatc gcattcacgg acgttctgcc
    11521 cacgggggcg tccatcatac tgatgccttt ttcttgtaac tcccgtatta atgcatcggt
    11581 ctgtaaggga tgaatagtcg acatatcaat caataacgcg tcagatgaaa cagcctcaca
    11641 gacgcctttt tcccccaaca gaacctgtcg aacaatgtca ccattgggaa gcatagtgat
    11701 gataaattca gcgcctgctg ccgcttccgc tggggtttgt gccgcagtgg ccccctgcgt
    11761 aactagcgca tcgacggcct gtgcattgac atcaaatacc tgcaatgaat gccctttttg
    11821 caatagattg ttagccattg ggctacccat ctgccccagc ccgagaaatg caatggttgc
    11881 catgaatact ctcctgtctg acattgacaa aataatgaca attgaaaatt cgttttcgtc
    11941 tgtttttgag catatttgta atttatgttc aaaaacacga cacgcatcac taatttgatc
    12001 atttgtgtca tttaaattga ccgaagacga caaaattaaa caggacaaga caaatatgac
    12061 caaaattgca tgcgtgggta tcacagttca ggacaggatt tattctttgc tcactctgcc
    12121 ggatggcggc ggaaaatatc aggcaaatca ttatcttgag attggtggcg gcccggcagc
    12181 aacagccgct gttgcaatcg caaagcttgg cgtagaggtt gatttcatcg gtcgcgtcgg
    12241 cgatgacagt tgtggaaata ccttacttgc agaacttgag ggttggggcg tcaataccgc
    12301 attttgccgc cgatatccta acgcccgttc atcgcaatca gcgatcctcg ttgatcagca
    12361 tggcgaacgg attatcgtta attatccaag tcccgatctc ggtactgatg ccgaatggtt
    12421 agaagcgatc gatttctctc gttatgatct gattctggcc gatgttcgct ggcatagcgg
    12481 tactgaaaaa gcattctcgc tagcgcgtct tgcgggtgtg acaacgcttt tagacgccga
    12541 tatgacaccg caggatattt ctccacttat cgcgcttgcc gatcacgccg tgttctcaac
    12601 accagggctg aaacgcatga ctggcctgca gtcacctgaa gaaggtttgt tccaggcaac
    12661 gacacaaaca gcaggtaaag tgtatgttac gctgggttct gaaggatcgt tgtggattga
    12721 agatggtcat ttgtgtcaac aggaggcatt ttctgtcaat gtggttgata ccactggagc
    12781 aggcgatgtt tttcacggcg cactcgcggt agcgttggca gaaaaaatgc caacaaaaga
    12841 ggctattcgt tttgccagtg cggttgccgc gatgaaatgt acccagcctg gcggtcgggc
    12901 cggcattcct aatcgtgagc aaaccgaatc atttttgtca ctttatgcgt aaaatggtaa
    12961 acatctaccg aggaggggca agggagtatt catgagtatc atcgaagtga caggtaatcc
    13021 acgccatgac caactcgttc atctaatcgc agaacgcggt tatatgaata ttgaagaact
    13081 ggcacaactg ctggatgttt caacgcaaac cgtgcgccgg gatatccgaa aactcagtga
    13141 gcagggcttg atcacccgtc atcatggagg ggccgggcgc gtctccagcg tcatgaatac
    13201 cgcttttgag caacgggaac tttcgctcac cgccgagaaa cgggcgatcg ctgaggcagt
    13261 cgccgattac cttcccgaac gctgtaccgt ctttatcacc atcggtacaa ccgtagaagc
    13321 cgttgccagg gcattactca accggcgtga tttacgcatt atcactaaca gcctacgtgt
    13381 ggcacagatt ctttataaga atcaggatat tgaagtgatg gtgccgggag gcactttacg
    13441 cgctcataac ggcgggatta tcggcccagg agccgtggat tttattgaag gcttccgcgc
    13501 agattattta atcaccagta taggtgccat tgaacatgac ggcaccctac tggaatttga
    13561 tgtaaatgaa gcgttagtcg cgagaacgat gattaaacat gcgcggaata cattgttagt
    13621 ggccgatcat acgaagtttg ccgcgtctgc tgccgtttca attggcaatg cacggaatgt
    13681 cagggctttt tttacagatg ccccgcctcc caattctttc tgccagttgt taagtgaaga
    13741 gaatgttgaa ctggtggttg ccgagcagga agtttcctga aattgaaact tcgaaaaatc
    13801 cgctggggat tcagcggatt ttattttgcc ggatgcggcc tacggttgat acggttttcg
    13861 ttggccggat taagcacatc cggccattaa tcaagcttca gccgccagca tctctttctt
    13921 acgttggcta attcgcttaa ccagcattac ggaaaccacc gcaccacaga atgcgaagca
    13981 ggccataatg ccaaacacga ttttgtagcc aataatcccc ggatttaaat ccagaatgta
    14041 gccatacaga ctgaagcaga acatcgccgg agcgtaacca ataaagctac ccaacgccat
    14101 cgccgcgcct gttttatttt cagcaatttt tgcttcgccg ataggtgcaa aaaatacggc
    14161 tcgctgtgta aagactatcg cgccaaagcc cagcgtacat gccatcccta aatagaccgg
    14221 catactttcg tgcggcagca taatcaacag tacgagcgca gcggtactga tgataaaggt
    14281 gtagcataga tatttactcg gcgatttcag gatcttatct gaaatcatgc caccaatcgg
    14341 tccaccaatc attttcagac agtattggtt aatgatgccg taagccccca ccagcgcaac
    14401 gggcaataga tagatgtttt tcaggaatgg aatgaagaat gtcaggccac agtaaaccgc
    14461 gtaaacgaag aagacgttaa aagcgataag ccagatggtt ttgtctctca gcaccgaggt
    14521 catactggtg ttttgcgatg ctccgtcttc ttttttcacc tcaacggacg gtgcctcttc
    14581 tttgtcattc aggacaaaga aaataataat gcctacggca atcacaataa gggaatagaa
    14641 ccagatgcct gctttaaaac ccaataagcc actgccaaac caggtaaata ctgccaacgc
    14701 agaaaatgcc accacggtat cgacaatgcc acgccctgtt tcgaagaagc caaacaaccg
    14761 accttgttgt tcgctattgc ccaatcgact taccgacttg agcaagaccg gccagttcat
    14821 catgtcgcaa gtaacgccaa atagcgccca gacaaagagg atgccccaat accccggcat
    14881 tgtcgtcaaa taaacaccca gtaatcctgt cgcaatgagt gagaatgaca tggtgtatct
    14941 gcgcggtagt ttatcggcaa aataaataga cagaaaaaaa cccactgtcg tgacaaatga
    15001 gtttaccgac atagcattac caatttgacc attggtcaaa tggaaatatt cctgcatcgg
    15061 gatataaaac gcatctttca gcgacggtaa tttataaatt gtaccgccac acaatgttaa
    15121 tagactaaat aacgcccatt tctttttcgt gagcataaca tcccctaagc gatttttaaa
    15181 atcactgttt taaaggcaat aagataccga ccattcatat ggaatggttt tattaactgc
    15241 gtatgagtta aaaattatta gtggttcaga agatggtttg cctcttgttt gatttgttgt
    15301 agtacgttac gcacatgatt tatctgatta attgcatctt ctttttcttt acgtaaccgc
    15361 gcatccagta aatgattttc tggcaatggt gtttcactca tctggcgata agggatccac
    15421 ggattatcat cttcgtcttc aaagcggaac gccggcgcat agtaatcaac ctcttcttcc
    15481 aggccatatg ccgtcacctg cggctcatca tcacccaggc agataaggtg cgttaataac
    15541 gctttgaacg gcatatcacc ctgacctgaa atacaggctt tatgacccag gccaccctgt
    15601 tctttaacga tcaaggcatc tttaatatgg acctgggtaa tatgcggtgc catcgttttt
    15661 aaggcgtcga tgggatgctc atttgcattg atcatgttcg caaaatcaaa cagtaaggaa
    15721 agagactcca tctcactttc tttgaccagc gacaccaact catgactttt taaatcttca
    15781 tgctgctcga tcgtaaaagt caggccgctg tcctgatacg tttcccgtac ataggcaatg
    15841 tcgttagcga taatcgataa tacgtcgcgc aaattacctt cataacgtgg ataaaaacgt
    15901 acggacgatg ccccagtttt caacgcgatg gcgacggctt cgtcaataga tgccttatct
    15961 gaggcgctgg tttcaatatg aatatcaagg ttcagacggc gggctttatc cccaaaggca
    16021 gagagttctt tatcgtccat attaccaaga gaaaaacgct cgccatccag aacatggatt
    16081 ttgacgccac gtagattatt ttcacttgcg atatcaagta aatcagcagg caacactctt
    16141 tccagccgca tatttaaatg aaaggcataa gcatgtaaat aaagaggaag agtgtcgaca
    16201 cgttgtagaa tctttcttgc gttatttatt gtcaccataa aacacctaca gattaatgat
    16261 gaggtctacg gcctcaaatc gccaatattc aatatctatc tggacaatat tgccttgctg
    16321 gtcggcgcga tgcttttcaa ttaagatcgc tggcataccc gcagaaccgc caagatactt
    16381 gctgatatcg cccggcattc tgacgggttt gaaaatcaat ttttttacta ccgtctcttt
    16441 ttggtagcac ttttcccaga ctgcagaaaa tgagtggttt tcaagttgtt caataaaacc
    16501 cggagcaact tcaggattaa tatatgtttc gtgatagaaa actttatgtc cttccagcgc
    16561 cccccagccg gtgatgcgat agagttcagt tgatggtgtc actgcaatca gtggcgcgag
    16621 cgtctcggga atatcgctgg tacggttttt ctcggtaaac ccccaggaag gctctcgtcc
    16681 ttgctcaatt gcggcccgct gaaagctcgc cgacagctcc ggactgtaat taaatcgcgg
    16741 ctgggtcacg aaccacccct tacgatcctt acggaagatt ttggattctg cctcgaggtt
    16801 caacaacgcc tggcgcagcg tcatacgttt aatgcccagc agttctccca gctctctttc
    16861 tgaagggagt tttccacccg gcgtggtgat gccatctttc aaccaccgat ccagtttctc
    16921 ttttgcattt tctacggtgg attgattctc cgccattgtt taatcctttt ggtttaaacc
    16981 agatgaggca tcttacgctt acgccaaaaa agaaagcaat taacggcgta tgactttgca
    17041 gataattgtg acaaagctct ctattgacgt aatcccagac ttataaggga gatactcgca
    17101 ttggtgtgcg ctcacgacag gtaaaaaaaa acctgccagc gatggcaggt tttttataac
    17161 taaaaaacga aattaatcgt ctttcggtgc gcggttggcg cgacgacgat cgttttccgt
    17221 caggtgacgt ttacgaatac ggatagaggt cggggtcact tctaccagtt cgtcatcatc
    17281 gatgaactcc agagcttgtt ccagagtcat gcggataggc ggaaccagaa caacggcttc
    17341 gtcagtaccg gaagcacgca tgttggtcag tttcttaccg gtcaggcagt ttacagtcag
    17401 gtcgttagag cggctatgaa taccgataat ctgaccttcg taaacttctg caccgtgacc
    17461 gaggaacagc ttaccgcgat cctgcagacc gaacagcgcg aacgcgaccg ctttaccctg
    17521 accgttagag atcagtacgc cgttctgacg ctgacccact tcacccggac gtacgtcgtc
    17581 gtagtggctg aaggtggagt acagcagacc agtaccggaa gtcatggtca tgaactcaga
    17641 acggaagcca atcagaccac ggcttgggat cacgtagtcg agacgtacgc ggcctttacc
    17701 gtctggattc atgtttttca ggtcgccttt acgttcgccc agcgcctgca ttacagaacc
    17761 ctgatgctgt tcttcaacgt ccagagtcac gttttcatat ggctcttgtt tacgaccgtc
    17821 gatttcacgg aagataactt tcggacggga taccgccagt tcgaaacctt cacgacgcat
    17881 gttttcgatc agaacagaca ggtgtagttc gccacgacca gaaacgcgga acgcatcggc
    17941 gtcttcggtt tcttctacgc gcagcgcaac gttgtgtacc agttctttgt tcagacgatc
    18001 caggatctga cgagacgtta cgaacttacc ttctttaccg cagaacggcg aggtgttaac
    18061 gcagaagaac atagaaacgg tcggctcatc aacggagagt gccggcagcg cttcaacgtt
    18121 ttgcgtgtcg caaacggtgt cagaaatgtt cagttcgcca aggcccgtga tcgcaacgat
    18181 atcgccagct tccgccaggt cggtttcgat acgttccaga ccgaggtggc ccagcacttt
    18241 accgactttc gcgttgcggg ttttgccttc gctatcgatg atagtgacct gctggttcgg
    18301 cttcacttta ccgcgcttga tgcggccaat gccgataacg ccaacatagc tgttgtaatc
    18361 gagctgagaa atctgcatct ggaacggacc gtcaaggtca acgtccggcg caggaacgtg
    18421 atcaacaatc gcctgataca gcggggtcat gtcttccgcc atatcttcgt ggtccagacc
    18481 cgcgataccg ttcagagcag aagcgtaaac gatcgggaag tccagctgct cgtcggtcgc
    18541 gtcgaggtta acgaacagat cgaatacctg atccacaacc caatcaggac gcgcgccagg
    18601 gcggtcaact ttgttgataa caacaatcgg cttcaggccg taagcaaacg cttttttggt
    18661 tacgaagcgc gtttgcggca tcgggccgtc aaatgcgtca accaccagca gcactgagtc
    18721 taccatggac attacacgtt caacttcacc accgaagtcg gcgtgccccg gggtatcaac
    18781 gatgttgata cggtaatcat tccatttgat agcggtgttt ttcgcgagga tggtaatccc
    18841 acgctctttc tccaaatcgt tggagtccat cacgcgctct tgggtttcgg cacgagagtc
    18901 gaacgtaccg gattgttgga gcagcttgtc taccagggtg gttttaccat ggtctacgtg
    18961 cgcgatgatg gcgatattac gcaatttttc gatcacaact ttgcctcagg cattagaaat
    19021 agcgcgttat tgtacacgga ttaatcgcac tacaaaacag gatcacaaac atcctccgca
    19081 aacaagtatt gcagagtccc tttgtgatcg ctttcacgga gcataaaaag ggttatccaa
    19141 aggtcattgc accaacatgg tgcttaatgt ttccattgaa gcactatatt ggtgcaacat
    19201 tcacatcgtg gtgcagccct tttgcacgat ggtgcgcatg ataacgcctt ttaggggcaa
    19261 tttaaaagtt ggcacagatt tcgctttatc ttttttacgg cgacacggcc aaaataattg
    19321 cagatttcgt taccacgacg accatgacca atccaggaga gttaaagtat gtccgctgaa
    19381 cacgtactga cgatgctgaa cgagcacgaa gtgaagtttg ttgatttgcg cttcaccgat
    19441 actaaaggta aagaacagca cgtcactatc cctgctcatc aggtgaatgc tgaattcttc
    19501 gaagaaggca aaatgtttga cggctcctcg attggcggct ggaaaggcat taacgagtcc
    19561 gacatggtgc tgatgccaga cgcatccacc gcagtgattg acccgttctt cgccgactcc
    19621 accctgatta tccgttgcga catccttgaa cctggcaccc tgcaaggcta tgaccgtgac
    19681 ccgcgctcca ttgcgaagcg cgccgaagat tacctgcgtt ccactggcat tgccgacacc
    19741 gtactgttcg ggccagaacc tgaattcttc ctgttcgatg acatccgttt cggatcatct
    19801 atctccggtt cccacgttgc tatcgacgat atcgaaggcg catggaactc ctccacccaa
    19861 tacgaaggtg gtaacaaagg tcaccgtccg gcagtgaaag gcggttactt cccggttcca
    19921 ccggtagact cggctcagga tattcgttct gaaatgtgtc tggtgatgga acagatgggt
    19981 ctggtggttg aagcccatca ccacgaagta gcgactgctg gtcagaacga agtggctacc
    20041 cgcttcaata ccatgaccaa aaaagctgac gaaattcaga tctacaaata tgttgtgcac
    20101 aacgtagcgc accgcttcgg taaaaccgcg acctttatgc caaaaccgat gttcggtgat
    20161 aacggctccg gtatgcactg ccacatgtct ctgtctaaaa acggcgttaa cctgttcgca
    20221 ggcgacaaat acgcaggtct gtctgagcag gcgctgtact acattggcgg cgtaatcaaa
    20281 cacgctaaag cgattaacgc cctggcaaac ccgaccacca actcttataa gcgtctggtc
    20341 ccgggctatg aagcaccggt aatgctggct tactctgcgc gtaaccgttc tgcgtctatc
    20401 cgtattccgg tggtttcttc tccgaaagca cgtcgtatcg aagtacgttt cccggatccg
    20461 gcagctaacc cgtacctgtg ctttgctgcc ctgctgatgg ccggtcttga tggtatcaag
    20521 aacaagatcc atccgggcga agccatggac aaaaacctgt atgacctgcc gccagaagaa
    20581 gcgaaagaga tcccacaggt tgcaggctct ctggaagaag cactgaacga actggatctg
    20641 gaccgcgagt tcctgaaagc cggtggcgtg ttcactgacg aagcaattga tgcgtacatc
    20701 gctctgcgtc gcgaagaaga tgaccgcgtg cgtatgactc cgcatccggt agagtttgag
    20761 ctgtactaca gcgtctaata gttgaagttg tactacccgg cgcaacaacg ccggaggttc
    20821 aaaccaggcc catctggcgt aattgttgca gccagtttga acacggacag cgcgcagaac
    20881 ccggagcgta cactggtacg tgaggagttc gagcactgcc caggttcaaa atggcaaata
    20941 aaatagcctg atgggactgg tttttagttg ccgtggaaat ttcagcccat ctctgcatgg
    21001 gcttttttct ccgtcaattc tctgatgctt cgcgcttttt atccgtaaaa agctataatg
    21061 cactaaaatg gtgcaacctg ttcaggagac tgctttatgg caacaggcac gcagcccgat
    21121 gctgggcaga tcctcaactc gctgattaac agtattttgt taatcgatga caacctggcg
    21181 atccattacg ccaaccctgc cgcgcaacaa ctgctcgccc aaagctcccg caaattgttt
    21241 ggtacaccgt taccggaact gttgagctac ttctcattaa atatcgagct gatgcaagaa
    21301 agtctggagg cggggcaagg ttttaccgat aacgaagtga cgctggtcat cgacgggcgc
    21361 tcgcatatcc tttctgtgac ggcccagcgt atgccggacg gcatgatcct gctggagatg
    21421 gctccgatgg ataaccagcg ccgcttaagt caggaacagc tacagcacgc ccagcaggtt
    21481 gctgcccgtg atttagtgcg cggcctggca catgagatta aaaatccgct tggcggttta
    21541 cgtggcgcgg cgcagctgct cagcaaagcg ttacctgacc catcactact cgaatatacc
    21601 aaagtgatta tcgaacaggc ggaccggctg cgaaatctgg tcgaccgtct gttggggccg
    21661 cagctgcccg gtacgcgcgt taccgaaagt attcacaaag tggctgaacg cgtggtaacg
    21721 ctggtgtcga tggaactgcc ggacaacgtg cggttgattc gtgattacga tcccagcctg
    21781 ccggaactgg cgcacgaccc ggatcaaatt gaacaggtct tgctgaatat tgtgcgcaat
    21841 gcgctacagg cgctggggcc ggaaggcggt gaaatcattc tgcgtacccg caccgcgttt
    21901 caactgacct tacacggcga gcgctaccgg ctggcggcgc ggattgatgt ggaagataac
    21961 gggccgggca ttccgcctca tttgcaggat acgctgtttt acccgatggt cagcggccgc
    22021 gaaggtggca ccgggcttgg cttatccatc gctcgtaatt tgattgatca gcattcaggc
    22081 aaaattgaat ttaccagttg gccagggcat accgagttct cggtttacct gcctatcagg
    22141 aaataaaggt gacgtttatg caacgaggga tagtctgggt agtcgatgac gatagttcca
    22201 tccgttgggt gcttgaacgt gcgctcgctg gagcgggttt aacctgtacg acatttgaga
    22261 acggcgcgga agtactggag gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata
    22321 tccgtatgcc gggaatggac gggctggcgc tgctcaagca gattaaacag cgccatccaa
    22381 tgcttccggt catcattatg accgcacatt ccgatctgga tgctgccgtc agcgcctatc
    22441 aacaaggtgc gtttgattat ctgcccaaac cgtttgatat cgacgaagca gtggcgctgg
    22501 ttgagcgcgc tatcagtcat taccaggaac agcagcagcc gcgtaatatt cagcttaacg
    22561 gcccaacgac cgatatcatc ggcgaagcgc cagccatgca ggacgtgttc cgtattatcg
    22621 gtcggctttc gcgttcttct attagcgtgc tgattaacgg cgaatccggc accggtaaag
    22681 aactggtcgc tcatgccctg catcgccaca gtccgcgcgc caaagcgccg tttatcgcgc
    22741 tgaatatggc agctatccca aaagatttga tcgaatcaga actgtttggc cacgagaaag
    22801 gcgcgtttac tggcgcgaat accattcgtc aggggcgttt tgaacaggcc gatggcggta
    22861 cattattcct cgacgaaatt ggtgatatgc cgctggatgt gcagacgcgt ttgctgcgcg
    22921 tgctggcaga cggtcagttt taccgcgttg gcggctatgc gccggtgaaa gtggatgtgc
    22981 ggattatcgc tgccactcac cagaatctcg aacagcgagt gcaggaaggt aagttccgtg
    23041 aggatctgtt ccaccgcctg aacgttatcc gcgttcatct gccgccgctg cgcgaacgtc
    23101 gggaagatat tccccgtctg gcgcgccatt ttttacaggt tgccgcgcgc gaactgggcg
    23161 tagaagcgaa gttactgcat ccggaaaccg aagctgctct gacgcgtctg gcgtggccag
    23221 gcaacgtgcg ccagctggaa aacacctgcc gctggctaac ggtgatggcc gccgggcagg
    23281 aagtgttgat tcaggatttg cccggcgaac tgtttgaatc aacggttgcg gagagtactt
    23341 cgcaaatgca accggacagc tgggcgacgc ttcttgcgca gtgggcagac agagcgctgc
    23401 gttccggtca tcaaaatctg ctttccgaag cgcagccaga gctggagcgg acgttactga
    23461 cgaccgcgtt gcgacatacg caggggcata aacaggaagc ggcgcggcta ctcggctggg
    23521 gccgcaacac cctgacgcgt aagttaaaag agctggggat ggagtgattc acagcttgtg
    23581 tgtaaagatt gattattgag cgcaaattgc tggtatttta cgctttactg ttccgataag
    23641 ttcagtatga tcttgcccgg aaaacgggga gagtcattat gctggaatca ataattaatc
    23701 tggtatcgag tggcgcagtt gacagccaca caccgcaaac tgccgttgct gccgtgctgt
    23761 gtgccgcaat gattgggctg tttagttgag tttcagattg ctgatgaagt gctctttgtt
    23821 catgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcaatattt tcaacaggtt
    23881 ttgtaggcct gataagcgta gcgcatcagg caatcttgtt ctcagcacat tgatggcgct
    23941 ttcgttttct actttgtaaa cgaagcgcca ttcactattt aaatcacccg tgagaactgc
    24001 tgcatccgcg ctttctggcg cagataggta tcaaagcaca tgcaaatgtt gcggatcagc
    24061 aagcgacctt tcgccgtcac ctgtattccc ttctcatcca catccaccag cccatctttt
    24121 gctaacgggg cgagcagctt gagatcttcc gcaaagtaat cagcgaagtg caaatcccac
    24181 tgtttctcaa taggggcgta atccagacgg aagttgcaga tgagcgactt aatcacatcg
    24241 cggcgaatac agtcatcacg cgttagcgca ataccacgcc acagcgcatt gccttgttca
    24301 tccacttgct gatagtactg cttcaactct ttctggttct gcgcgtagca gtcgccaatc
    24361 atgctgatgg cggaaacgcc catccccagc agatcggtat cgccctgagt ggtgtagccc
    24421 tggaagttac gatgcagcac gccttcacgc tgggccaccg ccagctcgtc atccggacgg
    24481 gcaaagtgat ccataccgat aaactgatag cccgattgcg tcaggaaggc gatggtttcc
    24541 tgcaggatat cgagtttttg ctgcggactc ggcaggtcag catctttgat tttgcgctga
    24601 gcagcaaaaa tggtcggcag atgcgcgtag ttaaagacac tcagacgatc ggggttcagc
    24661 tccgccacac gtttcagggt aaaggcgaaa ctctccggcg tctgtttcgg caggccgtaa
    24721 atcaggtcga tgttggtgga ggtaaaacca atctcacgcg catggttaag cagtgcaaag
    24781 atgaactctt catcctgctc gcggttaacc agacgttgca cttctttgtt gaagtcctgc
    24841 acgcccatgc tcaggcgatt aaagccttcg gcgcgtaaat gatcgagtac atccagttcg
    24901 atttcccgcg gatcgacttc gatcgaaatc tccgcatcgg cattgaactg gaagttttcg
    24961 cgcagcagct tcatcaggcg gctgatttgc gctttattca gatacgtcgg cgttccgccg
    25021 ccccagtgca attggctgac gtgacgcccg gcgaacagcg gtgcacgatg gacgatttct
    25081 tgctccagcg cgtccagata ctgatcggcc ttgtgctgct ggcgagtaac aatcttattg
    25141 caaccgcaga agtaacaaag cttatggcag aacgggatat gtacgtagag agataatgga
    25201 cgctcaggat agcgcgccac ggcttgtaaa aacgcctgtt cgccgaagtc ttctgaaaac
    25261 tccagcgcgg tcgggtacga ggtgtatcgt ggcccggaat agttatattt ctggatcagg
    25321 gccagatccc agtcgatttg ctgtacagac atgctcactc cttccgatgg cgtctctggc
    25381 ggctacggcg taccggcgga tgcccgtttc gggtcatcag ccggttgcgc agccactttt
    25441 gtcgccgcga caaccgctgt agtttaacga ataaccacac cagataacat ataaccggaa
    25501 gggttatcag cagaacggga aggcccacgg cggcaatccg ttagttgccc cgcagcagac
    25561 gcatcatgtc ttcttgcttc tcgtcttctt cctcttcttc gtcatcgtca taagagaggc
    25621 cgagtttctg catcaactca tcaatacgat ccagtttagc atccacccag gattgctctt
    25681 cggcactcag ggtttcgcct gcttccagac gttccagcag cgcatccaga cgctcatccg
    25741 tttccagtaa ttccaactcc gcctgcggtg aaagcatagg tttctcactc ttcggtttgt
    25801 gctgtttggt gactttttca gtcacgccca atggaatagg ggttttactg ccaatacgtg
    25861 gatcttttgg tgcgttctgg cctttgctgc ctgacgtggt gtttccgccc gctgcgcggc
    25921 tgcccggcgc atgaccacga cgttttttct gacgcttgcg atcacgagct tcctgatcca
    25981 gctcctcgcg tgtttttcga cgcgcttttg catgaccttt gctgcgtgag tttgaagatg
    26041 atggtttcat aatggttcgt cttttagccg ttttattcag tatagatttg cggcggaatc
    26101 taacagaaag caagcaaaga aaaaaggcga cagattactc tgtcgccttt tttcctgact
    26161 cataaccctt aacgggtctt acaatccttg caggccgcca acacaccctg tttttccttt
    26221 agccggaaca atccgtgttg attccatttc cgtttaatta cgtctccgga cgtttgtctt
    26281 cctgacaatc ctctgtcttc gcctcctggc gctcctgacc ctcatcctga gtcattgttc
    26341 ctgttagcgt cctcgctcta tgggtgctac tttacgcctt tgctttaaac aaacaatcaa
    26401 ccgccatcag tatttgcaca ttttcagagt tttatataaa agcaattcac tgatttatat
    26461 agcttatttg acatgcatcg cagaattttc tctgctattt cgcataaaga ctatcggcaa
    26521 tgtctcacaa agcgtatggc ttttacttac aacgctcacg ccgaaaaatc cgccttttat
    26581 tatggttcag gtataatcgc cacaaagctt tgactgacgg agacgaccgc tttgactaat
    26641 ttgaattatc aacagacgca ttttgtgatg agtgcgcctg atattcgcca cctaccttcc
    26701 gataccggaa ttgaagtggc ttttgcaggc cgttccaacg caggtaaatc cagcgcgctg
    26761 aacacgctga ctaaccagaa aagcctggct cgtacctcaa aaaccccagg gcgcacccag
    26821 cttatcaacc tgtttgaagt ggctgacggc aagcgtctgg ttgacttgcc tgggtacggt
    26881 tatgcggaag tcccggaaga gatgaagcgc aaatggcagc gtgcgctcgg cgaatacctc
    26941 gaaaaacgtc agagcctgca aggtctggtg gtgctaatgg atattcgcca tccgctgaaa
    27001 gatttggatc agcagatgat tgagtgggcg gtagacagca atatcgccgt tctggtgctg
    27061 ctgaccaaag cggacaaact ggcaagcggc gcacgtaaag cgcaattgaa tatggtgcgt
    27121 gaagctgtac tggcgtttaa cggtgatgtg caggttgaaa cgttttcttc gttgaagaaa
    27181 caaggcgtgg acaagctgcg gcagaaactg gatacctggt ttagcgagat gcagcctgta
    27241 gaagaaacgc aggacggcga ataattttct tgccttaatg cttgtgccgg atgtggcgta
    27301 tccggcccgt aaattcaata gattacaatt ctaccgttgg cctgataaac atagcgcatc
    27361 aggcattacg gatcttttct ttcgcccaat aaaaaacgcc ccagtcatta ctgactgggg
    27421 cggctaaaat attcagccaa atccgattac gtgaagtaaa aggtctgaaa gatagaacat
    27481 cttacctctg taccctacgc gactaacttt actctttttt gttcagttca gaaagcactt
    27541 tttgtaattt tttttcagtt gttgcatagg aatttctaat agccatcaca aaacgtgaca
    27601 gcttatgttg cttacttacg aaaaaagggc atgttcaggc gaatcttagt gcgcctgatc
    27661 ccagttttcg ccactcccca cttccaccag caacggcaca tccagacggg tacagttttc
    27721 catcagttga tgaatctgct tcgcgacggc atcaacatca tctttatgaa cttcaaatac
    27781 cagttcatcg tgtacctgca tgatcatacg tacacgcggt tgctcagcct gtaaccacgc
    27841 atcaacggca atcatcgccc gtttgataat gtcggcggcg gttccctgca ttggcgcgtt
    27901 aatggctgca cgttcagccg ctgcacgacg agcaccattg ctggatttga tatccggcag
    27961 atacagacgg cgtccgtcca gcgtttcaac gtagccctgc tctttcgcct gagcacgggt
    28021 gcgttccata tactccagca cgccagggta gcgttcgaag taaaggtcca tgtacttctg
    28081 cgcttcttta cgtggaatgt tcaattgccg cgccagaccg aaagcactca tgccataaat
    28141 cagaccaaag ttgatcgctt tcgcgctacg gcgttgctcg ctggtgacgg tttccagtgg
    28201 caaaccaaac acttctgccg ccgttgcccg gtggatatct tttccttccg cgaatgcggt
    28261 cagcaagcct ttgtcacgcg agagatgcgc cataatgcgc agttcaatct gcgagtagtc
    28321 cgctgagaca atcacataat cctctggcgc aataaacgcc tggcggatac gacgaccttc
    28381 ttcattacgc accggaatgt tttgcaggtt aggatcggtt gacgataaac gtcccgttgc
    28441 agttactgcc tggtgataag aggtatgcac acgcccggtt ttcgggttga tcatcagcgg
    28501 cagcttgtcg gtgtaagtcg actttagctt cgccagacca cgatactcca gaatcacttt
    28561 tggcaacgga tagtccagcg ccagttcttc cagtacctct tccgacgttg acggcgcgcc
    28621 acccggcgtt ttcttcagcg gtttaatgcc ctgtttttca aacagaatgg tttgtaactg
    28681 cttggtggaa gaaaggttaa attcctcacc tgcaatttca tgcgctttct tttccagctc
    28741 agccagacga agggtgagct cttcagaatg attgtgcagc actttcggat cgatcttcac
    28801 accgttacgt tcaatgcgtg aaagcaccgg caccagcggc atttcgatat tctcgaagac
    28861 gttcaacggc cctttgtgtt tttgcagatc tggccacatt ttcagatgca actgcaaggt
    28921 gacatctgca tcttcggcgg cgtaacgtcc ggcttcttcg agggcaatct ggttaaaggt
    28981 cagttgattt ttgcctttac ccgcaatctc ttcaaaagtg atggttttgt gcttcaacca
    29041 acgttccgcg aggctgtcca tatcgtgacg cccggcaacg ctattgagaa tgtaggactc
    29101 cagcatggta tcaaacgcaa tcccacgcag ctcaatgccg taattcgcca gaataccgcg
    29161 atcgtatttc aggttttgcc cgaccttcag cgccttttca tcttccagca gcggttttag
    29221 caactcgagt gcacgctcgc gagagatttg atcgggcgcg tcaagataat catgagcaac
    29281 cggaatatat gccgctacgc ctggctcgat agcaaaagaa agcccgacca ggttagcaga
    29341 gatgttatca aggctgtcgg tttcggtatc aaatgcaaat accggcgctt tttccagctt
    29401 cgcaatccac gctttcagtg tttcttcatc aaggatggtg acgtagttgt cataagaaat
    29461 caccgttgcc gtcacttctg gtgcttcgtc tgcaacactg gtttcctgtg gcttcgcggc
    29521 tggttttgcc cctttggcct gtaaccattt gcccgcttcg acatcagcag tccagcgttt
    29581 gaactcatac tttttgaaca gccccaacaa ctcttccgct gccggttgct gcacttccag
    29641 ttgttcacag gtcagctcca gttcaacgtc ggttttaatc gtcgccagct ggtatgagag
    29701 ataagcaact tctttgtttt gctcgagctt cgctgccatt gttttcgcgc cacggaagct
    29761 caacccagca attttttctg gctcggcata cagcgtatcc agcccgccca gaccttgcag
    29821 caatgcctgc gcggtttttt caccgacgcc cggtacgcca ggaatgttat cggaggagtc
    29881 acccatcagc gccaggaaat cgatgatcag ttctggcggc acgccgtact tattcaccac
    29941 ctcttccggt ccgaggatgg tattcgtcat ggtattgata agcgtaatat ttggcgtcac
    30001 cagctgcgcc atatctttat cgccagtgct gatcagcacc ggacgcccgg ctttttcggc
    30061 ttcgcgcgcc agagtaccga taacgtcgtc cgcttctacg ccagaaaccg ccagcagcgg
    30121 cagtcccatc gctttaacca tcgcgtgcag gggttcgatt tgtgctcgca gatcgttcgg
    30181 cattggcggg cgatgtgatt tgtaatgttc aaacagttca tcacgaaagg tttttccctt
    30241 ggcgtcaaag accaccgctg catgcgtcgg tttatattgc atgatcagac tgcgcagcat
    30301 gttgaggaca ccatacatcg caccggtcgg ctcgcctgcg ctgttagtca gcgggggaaa
    30361 cgcgtgatat gcgcgataaa gataagatga accatctaca aggataagtg gattttgggg
    30421 gatctgaacc ataatgtccg tgcctgttta tcagattatg ggtaaaggat gccacagaca
    30481 ggatgaaaac atgagttttt caccgctttt cgtgaaaatc ttttgtagat cttctggatc
    30541 gctcgcaaat cgtcatgtgg ataactttgt gcataaatta tttacatcgc
//
LOCUS       DAPGWD010000041        30222 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPGWD010000041 DAPGWD010000000
VERSION     DAPGWD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.041"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="RHX88_001810"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="RHX88_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDV2230505.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="RHX88_001811"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="RHX88_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV2230506.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="RHX88_001812"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="RHX88_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDV2230507.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="RHX88_001813"
     CDS             complement(1087..1581)
                     /locus_tag="RHX88_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDV2230508.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="RHX88_001814"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="RHX88_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDV2230509.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="RHX88_001815"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="RHX88_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDV2230510.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="RHX88_001816"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="RHX88_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDV2230511.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="RHX88_001817"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="RHX88_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDV2230512.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /locus_tag="RHX88_001818"
     CDS             complement(6745..7032)
                     /locus_tag="RHX88_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDV2230513.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /locus_tag="RHX88_001819"
     CDS             complement(7151..8038)
                     /locus_tag="RHX88_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV2230514.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="RHX88_001820"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="RHX88_001820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDV2230515.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="RHX88_001821"
     CDS             complement(9275..9769)
                     /locus_tag="RHX88_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDV2230516.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="RHX88_001822"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="RHX88_001822"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV2230517.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="RHX88_001823"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="RHX88_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDV2230518.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="RHX88_001824"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="RHX88_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV2230519.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="RHX88_001825"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="RHX88_001825"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDV2230520.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /locus_tag="RHX88_001826"
     CDS             13861..14520
                     /locus_tag="RHX88_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2230521.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="RHX88_001827"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="RHX88_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDV2230522.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="RHX88_001828"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="RHX88_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDV2230523.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="RHX88_001829"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="RHX88_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDV2230524.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="RHX88_001830"
     CDS             17948..18874
                     /locus_tag="RHX88_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDV2230525.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="RHX88_001831"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="RHX88_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDV2230526.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="RHX88_001832"
     CDS             complement(19225..21444)
                     /locus_tag="RHX88_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDV2230527.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="RHX88_001833"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="RHX88_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDV2230528.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="RHX88_001834"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="RHX88_001834"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDV2230529.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24013..26271)
                     /gene="parC"
                     /locus_tag="RHX88_001835"
     CDS             complement(24013..26271)
                     /gene="parC"
                     /locus_tag="RHX88_001835"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDV2230530.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26817..27299)
                     /locus_tag="RHX88_001836"
     CDS             complement(26817..27299)
                     /locus_tag="RHX88_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2230531.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27352..27744)
                     /gene="ygiW"
                     /locus_tag="RHX88_001837"
     CDS             complement(27352..27744)
                     /gene="ygiW"
                     /locus_tag="RHX88_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDV2230532.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27896..28555
                     /gene="qseB"
                     /locus_tag="RHX88_001838"
     CDS             27896..28555
                     /gene="qseB"
                     /locus_tag="RHX88_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDV2230533.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28552..29901
                     /gene="qseC"
                     /locus_tag="RHX88_001839"
     CDS             28552..29901
                     /gene="qseC"
                     /locus_tag="RHX88_001839"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDV2230534.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLNNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29947..>30222)
                     /locus_tag="RHX88_001840"
     CDS             complement(29947..>30222)
                     /locus_tag="RHX88_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HDV2230535.1"
                     /translation="YWLIDAFFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFF
                     LLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         7448 a         8087 c         7639 g         7048 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccatgaca gatcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catactgtgc gaaagcggtg acatcagcga acaggagagg
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcgcc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgcgctctt
     1381 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg ccccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacg accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttaacaa
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagc gaccccattg
     3901 aagactggtg catggtcggc agcagcgcac cgagcgcgat gatgaagaac atcaccttgt
     3961 ttagtcgctg tagcgacacc ttccacccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtgccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtagcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagttggtt
     9061 gctgaaagat gagcgagtga cgtcggtatt gattggtgcc agccgcgcgg agcaacttga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcataa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc cgatctggac agcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagcaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac ctgttgcaat agattttgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aggtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtcccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttccattca ggccgctacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tgcgggagaa tgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc agtaatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccccaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctagccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgacg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgctg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gcattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgc ttgtcgccct tcggtacgaa
    19561 gacgtcttca ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga aagaacagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcac agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaaca cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gtggctgcac ggttacggct ttggcttcct gctttttcgg
    20641 tgccaccggt ttgttatccg cgcacggcaa atcttcacca tacggatgcg ggattgggtc
    20701 gatttttcca ggggtatcaa gacgggtgga atccacgccg ctccagccag gcagcgcctc
    20761 tttcacgata atcgcggtat tacgcacatc gcggatttca ctaatcggct cgcccatcgc
    20821 cagacggtgc gccacctcca ccagcggacg ctcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgtgcggt
    20941 acggcgcaga ctagcctcaa taccgccgag gatcaccggt acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccgggcgc ttacccgcga cgttatccgg
    21061 cgtgtaggca tcgtcatgac gtaaacggcg atcggcggta taacggttga tcatcgaatc
    21121 catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca tgaaatcgtc
    21181 tttgctgctc cagtctggct gggcgatgat cccgacgcga aagccctgtg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaaaagtt gccctatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagttgt ccattaatac ccgggtcatc acccagactg
    21901 atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accagaatgc tggatggctc aaagaagcca cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gataacgacg agagttcgac
    22261 gcgttcagca ggcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctga ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaataggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcgggcc tggtacctgt agcccggcta ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag cagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacagcgcc
    24001 ctccccggca tattactctt cgctatcacc gctgctggca cggcgaggag agtcgatctc
    24061 aacacgatcg atacgctgca aaccgcgcat caacgtaccg cggcgtccac gttcgccagt
    24121 gactttctgt agctcttccg gacgcagttt aattttgcgt ttcccaacat gaatggtcag
    24181 cgtgctttgc ggcggcagaa cgtacaattg cgccagacca tcttctccac gcgcggcttc
    24241 tgccgatgga atgttgataa tcttgttgcc tttgcccttc gacagctgcg gcagatcgct
    24301 taccgggaac atcaacatac ggcctgcctg agtgattgcc agcagcatat cggaagcatc
    24361 ttcaatcacc accggcggca taacatgggc attttccggt aaggtgatca aagccttacc
    24421 tgcacggtta cgcgccacca gatcgttaaa ggtgcagacg aaaccgtaac ccgcatcgga
    24481 agccatcagc agtttctgat cgtcgctttc catcagcata tggtcaacgg tcgccccagg
    24541 cggcaacgtt aatttgccgg tgagcggctc gccctgacca cgcgccgacg gcagcgtaat
    24601 cgggtcaatg gcatagctac gaccggtgga atcaacaaac actaccggtt ggttgctctt
    24661 acctttcacc gccgctttga agctatcacc cgctttataa ttcaggcccg gcgcgtcgat
    24721 atcatggcct ttagcgctgc gtacccagcc catctgcgac agcacaatgg tgacaggttc
    24781 agacggcagc atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct gcaacggcga
    24841 acgacgatcg tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca gcaggttatt
    24901 cattttacgc tcggaagcca aaatgccctg caactggtcg cgctcttttt ccagttcact
    24961 ctgctcaccg cgaatcttca tctcttccag tttggcaaga tgacgcagtt tcagttcgag
    25021 gatcgcttcc gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg gtttcggttc
    25081 atcttcatta cgaatgatct caatcacttc gtcgatattg agaaacgcca ccagcaaacc
    25141 ttcgaggata tgcaggcgct tgaggacttt ctccagacga tagttcagtc ggcggcgcac
    25201 ggtatcgcga cggaacacca gccattcgga gaggatttcc agcaggtttt tcaccgccgg
    25261 acgaccatcc agaccgatca tattgaggtt aatgcgatag ctcttttcca gatcggtggt
    25321 agcgaagagg tggttcatca cctgatccat atccacgcgg ttggaacgcg gcacaatcac
    25381 cagacgggtc gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca tcggcagctt
    25441 tttgttgcgc atttgcgcag caatttgctc cagtacgcgc gcacctgaaa cctgatgcgg
    25501 taatgcgctg ataaccaccg cgccatcttc tttcttccac accgcgcgca tacgcactga
    25561 accacgtccg ttctcgtaga ttttacggat ctcggcgcgc gaagtgataa tttccgcttc
    25621 agtcggataa tccggcccct gcacgatatc cagcagctga tcgagcgtgg ttttcggctg
    25681 gtcgattaat gcgattgccg cctgagctac ttcacgcagg ttatgcggtg gaatatcggt
    25741 cgccatgccg acggcaatac cggtggtgcc gttaagcaaa atgtttggca gacgagcagg
    25801 cagcattttc ggctcctgca aagtgccgtc gaagtttggc acccagtcag ccgtcccctg
    25861 ccccagctcg ctcaatagca gctcggaata tttcgacaat cgggattcgg tgtaacgcat
    25921 tgccgcgaac gatttcggat cgtccggcgc gccccagttc ccctgaccat caaccagcgg
    25981 ataacggtaa gagaacggct gcgccatcag gaccatcgct tcataacagg cgctatcgcc
    26041 gtgcggatgg tatttaccca gtacgtcacc gacggtacgg gccgattttt taaatttggc
    26101 gctggcattc aggcccagtt cagacatcgc atacacaatg cggcgctgaa caggtttaag
    26161 accatcgcca ataaacggca acgcacggtc catgatcacg tacatggagt agtttaagta
    26221 ggcgttttcc gtaaattcat gtagcgcaag gcgctctgcc atatcgctca ttaattctga
    26281 ttcctcaact tattcgccag ccttcaaagg ggcaatattg ccggagatac tacattatct
    26341 gccgcgtcga gtcacaggag atcagcgtga atagcgccgc cggacgtgct ccgacggcga
    26401 taaaggcatc aatgtgcctt gatatacaac tctttgctgt agacttgatc ggcatagagg
    26461 gcagcacaag gttgcgccca tgccacacca ctcggcatgg gggtccgctc cgggcgattc
    26521 gagaagttga ggttatgcga gtccagggaa gcctaaagca tcaaagtagt cgactgtaag
    26581 tatacggttc agcccagttt agctgtgtct ccaccagtgc cccgcgcctc ctgccataag
    26641 tgcggcttgc tcatggatac ccccgagacg gcttacggcg caccggataa taaatttcgc
    26701 gtatacatgg tattccttac cggagaaatt ataagcccaa caacaatacg cctgtggcat
    26761 tgttcgctca aacttatcgc gagtgatttg gctcacactc cggtaaagat tccccatcac
    26821 gccaacggca cataaacatc cgtttccagt cgctgctctg tcaccccttc ggcaagattg
    26881 gtgtagtgaa acagaatcgg tgctttacgc attttctcgc cgcttgcagg cagccagtgg
    26941 cgaataatgc cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg gcgggccacg
    27001 gcataacgtc caccggtaag ttcaccattg ctcacaccat aacgattatc gggaatcggt
    27061 tcgctaacgc tgccgcagat atcaaagcga aacgcttccg gtgcggtggt ggcagggtcg
    27121 tcccaggcga cgccaaaagt ctggctttgg ttaacaggag aaagccccgt ctctttacgc
    27181 cacatgataa atttcgccac actgtagttg agcaactcag ggctacagcg gtgcggcaac
    27241 atcgccacag ggattgatgg gaaatcgata atgtttacat ccagtgtcag gtttgtcatt
    27301 ttgcctccct gcgaaattat tcccgggaac gcgtcccggg agcggtaaca attacggatt
    27361 tactttgcgg atctgtttga cgtcaatttc aacagaattc cagtctttat cgacttcacc
    27421 ctgaatctca accgtatctt tcggcgtcac cgtcacaccg ttccagcgtt tgtggtcgat
    27481 atcaacattg atagtaccgc tggcatcttt aaacacgtag agatcgtcag agatgcgttc
    27541 aacgatattg ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg cgctttctac
    27601 ggtcgttacg ctgccgttcg gcccctggaa tcctccggcc tgactttgcg ttgccgatgg
    27661 gccagaaaaa ccgccctgct ctgctgccat caccggtgcg ctgcacaggg ccattactgc
    27721 gattactgct gcgaattttt tcatgtttat tactcccttt aatgtctgtt tccgagcatt
    27781 taacaagata gtccttaaca acttcttaag ggaaaaaaat aaaatttagt gctgtacaga
    27841 gcgcgttaca acacggttta ctggcagcaa atacggttat cgcagggatg aaaaaatgcg
    27901 aattttactg atagaagatg acatgctgat tggcgacggc atcaaaacgg gccttagtaa
    27961 aatgggtttt agcgtcgact ggtttacaca aggtcgtcag ggaaaagagg cgctttatag
    28021 cgcgccttat gatgcggtga tcctggattt aaccttacca ggcatggatg gtcgcgatat
    28081 tttgcgcgaa tggcgagaaa aaggtcagcg tgagccggta ctgatcctga ccgcgcgcga
    28141 tgcgctggag gaacgtgtag aagggctgcg tctgggagct gacgattatc tgtgtaaacc
    28201 ttttgcgttg atagaagtcg ccgccaggct ggaagctctg atgcgccgaa ccaacggcca
    28261 ggccagcaac gagctgcgcc acggtaacgt catgctcgac cccggcaaac gtatcgccac
    28321 gctggctggc gaacccttaa cactgaaacc aaaagaattt gccctgctgg aattactgat
    28381 gcgtaacgct ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt atacctggga
    28441 cgaagaggtc accagtaatg ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg
    28501 tagtgatttt attcgtaccg tgcatggtat tggctacaca ttaggtgaga aatgaaattt
    28561 acccaacgtc ttagtctgcg cgtcaggctg acgctaatct ttttaattct ggcctcggtg
    28621 acctggctgc tttccagctt tgtcgcctgg aaacaaacaa cggataacgt cgatgaattg
    28681 ttcgacaccc aactgatgct gtttgccaag cggttaagta cgctcgatct caacgaaatc
    28741 aacgcggcgg atcgcatggc acagacgcca aataaattaa aacacggtca tgttgatgac
    28801 gatgcgctga cctttgccat ctttacccac gacggcagaa tggtccttaa cgatggcgat
    28861 aacggagaag atattcctta tagctatcaa cgggaaggtt ttgctgacgg gcaactggtg
    28921 ggcgataaag atcaatggcg ttttgtctgg atgacctcac ctgatggcaa atatcgcatc
    28981 gttgttggtc aggaatggga ataccgtgaa gacatggcgc tggcgattgt tgccgggcaa
    29041 ttgatcccgt ggctggtcgc actgccgatt atgttaatca tcatgatggt actattgggt
    29101 cgtgaactcg cgccgctgaa caaactggcg ctggcgctgc gtatgcgtga ccctgactcg
    29161 gaaaaaccac taaacgcgac tggcgtaccc agcgaagtgc gtccgttggt tgagtcgcta
    29221 aatcaactgt tcgcccgcac acatgcgatg atggttcgtg aacgacgctt tacctccgac
    29281 gcagctcacg aactacgtag cccgttaacg gcgctgaaag tgcaaaccga agttgcgcag
    29341 ctttctgacg atgatccgca agcgcggaaa aaagcgctgc ttcaattaca ttacgggatc
    29401 gatcgcgcta ctcgtctggt tgatcaactg ctcactctat cgcggctgga ctcactgaat
    29461 aaccttcagg acgtcgcgga gatcccgctt gaagatctcc tgcaatcgtc ggtgatggat
    29521 atttaccaca cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa tgcccacggc
    29581 atcaaacgca ccggacaacc gctattgcta agtttgttgg tgcgaaattt gctggataac
    29641 gccgtgcgct acagtccaca gggcagcgtg atagacgtca cgctgaatgc tgataatttc
    29701 atcgtgaggg ataacggccc cggtgtgacg ccagaggctc tggcgcgaat tggcgaacgc
    29761 ttctatcgcc cccccggaca aaccgctacc ggcagtggac ttgggctatc gattgtccag
    29821 cgaatcgcca aattgcatgg catgaatgtt gagtttggga atgcggaaca aggtggattt
    29881 gaggcgaagg taagctggta acttctctgt ctatgagagc cgttaaaacg actctcatag
    29941 attttactaa tagcaaaata taaaccgtcc ccaaaaaagc caccaaccac aaaccagaca
    30001 aataccgagc gagaatacat atacccaaaa ctcgaacatt tcccgcataa agagttttct
    30061 taagataaga ataataagtg gcgtaagaag aaaaaatgct gcaatcattg cttgcaatat
    30121 acgatcttcg cctgaaacgg aaggtatact gcaaatattg tctcccgact caagcatcca
    30181 gtcatactta ttgaaagcaa aaaaagcatc tattaaccaa ta
//
LOCUS       DAPGWD010000042        30186 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPGWD010000042 DAPGWD010000000
VERSION     DAPGWD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.042"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1522
                     /gene="mltF"
                     /locus_tag="RHX88_001841"
     CDS             <1..1522
                     /gene="mltF"
                     /locus_tag="RHX88_001841"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDV2230536.1"
                     /translation="ILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTY
                     NEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSE
                     RVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKF
                     PELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVT
                     WFSPLDGDNTLSAALLDFFNEMNEDGTLTRIEEKYLGHGDDFDYVDTRTFLRAVDAVL
                     PQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDR
                     TDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGN
                     PDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQA
                     TEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN"
     gene            complement(1519..2055)
                     /gene="tadA"
                     /locus_tag="RHX88_001842"
     CDS             complement(1519..2055)
                     /gene="tadA"
                     /locus_tag="RHX88_001842"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDV2230537.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(2080..2715)
                     /gene="yfhb"
                     /locus_tag="RHX88_001843"
     CDS             complement(2080..2715)
                     /gene="yfhb"
                     /locus_tag="RHX88_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDV2230538.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2924..3772
                     /locus_tag="RHX88_001844"
     CDS             2924..3772
                     /locus_tag="RHX88_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV2230539.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3828..4088
                     /gene="yfhL"
                     /locus_tag="RHX88_001845"
     CDS             3828..4088
                     /gene="yfhL"
                     /locus_tag="RHX88_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDV2230540.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(4282..4362)
                     /gene="shoB"
                     /locus_tag="RHX88_001846"
     CDS             complement(4282..4362)
                     /gene="shoB"
                     /locus_tag="RHX88_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDV2230541.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(4782..5162)
                     /locus_tag="RHX88_001847"
     CDS             complement(4782..5162)
                     /locus_tag="RHX88_001847"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDV2230542.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(5162..5893)
                     /gene="pdxJ"
                     /locus_tag="RHX88_001848"
     CDS             complement(5162..5893)
                     /gene="pdxJ"
                     /locus_tag="RHX88_001848"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDV2230543.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(5905..6633)
                     /gene="recO"
                     /locus_tag="RHX88_001849"
     CDS             complement(5905..6633)
                     /gene="recO"
                     /locus_tag="RHX88_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDV2230544.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(6645..7550)
                     /gene="era"
                     /locus_tag="RHX88_001850"
     CDS             complement(6645..7550)
                     /gene="era"
                     /locus_tag="RHX88_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDV2230545.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(7547..8227)
                     /gene="rnc"
                     /locus_tag="RHX88_001851"
     CDS             complement(7547..8227)
                     /gene="rnc"
                     /locus_tag="RHX88_001851"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDV2230546.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(8500..9474)
                     /gene="lepB"
                     /locus_tag="RHX88_001852"
     CDS             complement(8500..9474)
                     /gene="lepB"
                     /locus_tag="RHX88_001852"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDV2230547.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(9490..11289)
                     /gene="lepA"
                     /locus_tag="RHX88_001853"
     CDS             complement(9490..11289)
                     /gene="lepA"
                     /locus_tag="RHX88_001853"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDV2230548.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(11487..11966)
                     /gene="rseC"
                     /locus_tag="RHX88_001854"
     CDS             complement(11487..11966)
                     /gene="rseC"
                     /locus_tag="RHX88_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDV2230549.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(11963..12919)
                     /gene="rseB"
                     /locus_tag="RHX88_001855"
     CDS             complement(11963..12919)
                     /gene="rseB"
                     /locus_tag="RHX88_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDV2230550.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(12919..13569)
                     /gene="rseA"
                     /locus_tag="RHX88_001856"
     CDS             complement(12919..13569)
                     /gene="rseA"
                     /locus_tag="RHX88_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDV2230551.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(13602..14177)
                     /gene="rpoE"
                     /locus_tag="RHX88_001857"
     CDS             complement(13602..14177)
                     /gene="rpoE"
                     /locus_tag="RHX88_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDV2230552.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(14174..14329)
                     /gene="rseD"
                     /locus_tag="RHX88_001858"
     CDS             complement(14174..14329)
                     /gene="rseD"
                     /locus_tag="RHX88_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDV2230553.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            14585..16207
                     /gene="nadB"
                     /locus_tag="RHX88_001859"
     CDS             14585..16207
                     /gene="nadB"
                     /locus_tag="RHX88_001859"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDV2230554.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(16192..16929)
                     /gene="trmN"
                     /locus_tag="RHX88_001860"
     CDS             complement(16192..16929)
                     /gene="trmN"
                     /locus_tag="RHX88_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDV2230555.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            17061..18395
                     /gene="srmB"
                     /locus_tag="RHX88_001861"
     CDS             17061..18395
                     /gene="srmB"
                     /locus_tag="RHX88_001861"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDV2230556.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(18604..19485)
                     /locus_tag="RHX88_001862"
     CDS             complement(18604..19485)
                     /locus_tag="RHX88_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2230557.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELG
                     EQPLVLVASPQIADIDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVGNELASGELIELPFGEQTQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFVAG"
     gene            19588..20175
                     /gene="eamB"
                     /locus_tag="RHX88_001863"
     CDS             19588..20175
                     /gene="eamB"
                     /locus_tag="RHX88_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDV2230558.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(20230..20613)
                     /gene="grcA"
                     /locus_tag="RHX88_001864"
     CDS             complement(20230..20613)
                     /gene="grcA"
                     /locus_tag="RHX88_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDV2230559.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            20918..21607
                     /gene="ung"
                     /locus_tag="RHX88_001865"
     CDS             20918..21607
                     /gene="ung"
                     /locus_tag="RHX88_001865"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDV2230560.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(21655..22692)
                     /locus_tag="RHX88_001866"
     CDS             complement(21655..22692)
                     /locus_tag="RHX88_001866"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV2230561.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            22899..23318
                     /gene="trxC"
                     /locus_tag="RHX88_001867"
     CDS             22899..23318
                     /gene="trxC"
                     /locus_tag="RHX88_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDV2230562.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            23387..24085
                     /gene="tapT"
                     /locus_tag="RHX88_001868"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             23387..24085
                     /gene="tapT"
                     /locus_tag="RHX88_001868"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDV2230563.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTDPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGELFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            24117..26777
                     /gene="pat"
                     /locus_tag="RHX88_001869"
     CDS             24117..26777
                     /gene="pat"
                     /locus_tag="RHX88_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDV2230564.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            26891..28246
                     /gene="pssA"
                     /locus_tag="RHX88_001870"
     CDS             26891..28246
                     /gene="pssA"
                     /locus_tag="RHX88_001870"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDV2230565.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGIPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            28292..28615
                     /locus_tag="RHX88_001871"
     CDS             28292..28615
                     /locus_tag="RHX88_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDV2230566.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(28612..29910)
                     /gene="kgtP"
                     /locus_tag="RHX88_001872"
     CDS             complement(28612..29910)
                     /gene="kgtP"
                     /locus_tag="RHX88_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDV2230567.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT         7385 a         7931 c         7523 g         7347 t
ORIGIN      
        1 cattctggca ctgctgctcg cggtcgctct ctggccatcc attccctggt ttggtaaagc
       61 cgacaaccgt atcgccgcca ttcaagcgcg gggagagttg cgtgtgagca ccattcatac
      121 tcccctgact tataacgaaa tcaacgggaa accttttggc ctggattacg aactggcgaa
      181 acagtttgcc gattacctcg gcgtaaaact gaaagtgacc gtgcggcaga atatcagcca
      241 gctgtttgac gaccttgata atggtaacgc cgacctgctg gcggcaggac tggtctataa
      301 cagtgagcgg gtaaaaaatt atcagcctgg ccctacctat tattccgtgt cacaacaact
      361 ggtttataaa gtgggtcagt atcgcccacg tacgctgggc aacctgacgg cggagcaact
      421 caccgttgca ccgggtcatg tggtggttaa cgatctccag accctgaaag aaacaaaatt
      481 cccggaatta agctggaagg tagacgacaa aaaaggctct gcggaattaa tggaagatgt
      541 catcgaagga aaactcgatt acaccattgc tgattctgtc gccatcagcc tgtttcagcg
      601 cgttcacccg gagctcgccg tagcgctcga tatcaccgat gaacaaccgg tgacttggtt
      661 tagcccgtta gatggcgata ataccctttc cgccgccctg ctcgacttct tcaacgaaat
      721 gaatgaagac ggtacgctga cacgcattga agagaaatac ctggggcatg gcgatgattt
      781 tgattacgtc gatacgcgca catttttacg cgccgtcgat gcggtactgc cgcagttaaa
      841 gcccctgttt gagaaatacg ccgaagaaat tgactggcgt ttgctggccg ctattgctta
      901 tcaggaatcg cactgggatg cacaggccac ttcaccgacg ggtgtgcgcg gcatgatgat
      961 gttaaccaaa aataccgcgc aaagcctcgg cattacggat cgtaccgatg ccgaacagag
     1021 catcagcggt ggcgtgcgtt atttgcagga tatgatgagt aaagtgccgg aaagtgtgcc
     1081 ggagaacgag cggatctggt ttgccctcgc tgcgtacaat atgggctatg cgcatatgct
     1141 ggatgcccgc gccctgacgg caaaaaccaa agggaatcct gacagttggg ctgacgtaaa
     1201 acagcgtctg cctttactta gccagaaacc ctattacagc aagctgactt acggctacgc
     1261 tcgtggacat gaagcctacg cttatgtcga aaatattcgt aagtatcaga ttagcctggt
     1321 gggttatctg caagagaaag agaagcaggc tacagaagcg gcgatgcaac tggcgcagga
     1381 ttatccggcg gtatcgccta cggagttggg caaagagaaa tttccttttc tctcgtttct
     1441 ttcccagtcg tcatcaaact atttgaccca ttctccctct ctgctgtttt ccaggaaagg
     1501 gagtgaagag aaacaaaatt aatccgtcga ggattgcgct tttttctgcg ctttaatttc
     1561 ctggcggcgc atgcgaaaga agtcactgag caacgccgcg cactcatccg ccagtattcc
     1621 ttccgtaatt tccactcggt gattcatacc cggatgatgc agcacatcca ttaaagatcc
     1681 cgcagcgcca gttttcgcgt cacgcgcacc aaagaccacg cgaccaatgc gactgtggat
     1741 catcgctccg gcacacatta cacatggttc aagcgtgaca tacaacgtgg cgtcgatcag
     1801 acgataattt tgcatcacca gaccaccctg ccgcagggcc atgatttctg catgtgcggt
     1861 gggatcatgg cgaccaatcg ggcggttcca gccttcgccg attacccgat tgttatgcac
     1921 taataccgcg ccgaccggca cttcccgctc atcccaggca cgtttcgcca gcgtcagcgc
     1981 gtgacgcatc cagtattcgt ggctaaattc gacttcagac aaaaagaaaa ctccggttat
     2041 aaaagcgcgg cgcattatac acggacgcta tgctttactc tattccagtt gctggagttc
     2101 accgcgcggg gttactcgcc aacgatgctg acagaaataa agcagcggat tgtcctgatt
     2161 actgtcgcta tagccactgt acagccgcag cggagtgccg attttgcgct ccagttgtgc
     2221 gaccttttca tgtcccagac aacgcatcgt caatacccaa ccaccatagc cacgctgaat
     2281 ttggctggcg ataagattaa cccgcggcag ccagggcgta tcgaaataaa ccgcttcaac
     2341 cagcggctgc ggagagccgg taatcaacca gatatcagca tcggaactta acaggtaggt
     2401 ggttaatcgc tcctgaacca gcggaaaggc ggtaacattg tcgcgaaacc agcgcacgaa
     2461 atcggcctgc aacgtctgta aacgtgcttc gctgtgacca aaagtgcacc cccacagaag
     2521 cagactcatc ggccagcgtg ccgcacgacc ttttatcaat aacgcaatgg ctataatcgg
     2581 taacaacggc aggacaagta acgcattcag cggttggcga cgcagtaaat agcgcagaaa
     2641 actgccgaac atatcctgct gatgcaatgt tccatctaag tcaaaaaaca ccacacgacg
     2701 ctcgtgagtt gccaaaccat actcctctgg actgaaacat cctgattaat tttcgctact
     2761 acatagccta acagatagat catcactttt ccggcaacaa tcgaccgcat catggctaac
     2821 tggaattttt aattcatggc aattagcggc aatggaatat aaaattcact cgcgtgtctc
     2881 tcatattgcc tgttgtcgcc actatttcat cgcgaggaaa gatatgaacg gcttacttcg
     2941 tatccgtcag cgttaccagg ggcttgccca gagtgataaa aaactggcgg attatctgct
     3001 gctacaacct gatacggcgc gccatttaag ctctcagcaa ctggccaacg aagccggagt
     3061 cagtcagtcc agcgtcgtga agttcgcgca aaaactcggc tataaaggtt ttccggcgct
     3121 taagttggcg ttgagtgaag cgctggcaag ccagccggaa tcgccctccg tgcccattca
     3181 taaccaaatc cgcggtgatg atccgttacg gctggtcggc gaaaaactga ttaaagaaaa
     3241 tactgccgct atgtacgcaa cgctaaacgt taatagtgaa gagaaactgc atgaatgcgt
     3301 aacaatgttg cgctctgcgc ggcggataat tctgaccggt attggcgctt cgggtctggt
     3361 ggcgcaaaac tttgcctgga agctgatgaa gattggcttc aatgctgccg cagtgcgcga
     3421 tatgcatgcg ctactcgcaa cagtacaggc gtcgtcccct gacgatctgt tattagccat
     3481 ttcctacacc ggtgtacgac gcgagttaaa cctggcggca gatgagatgc tgcgagtggg
     3541 cggaaaagtg ctggcgatta ccggctttac tccgaatgcc ctgcaacagc gtgcttctca
     3601 ttgcctgtat accattgccg aagaacaagc gacaaacagc gcttcaatct ctgcttgtca
     3661 cgctcaggga atgttaacgg atttgctgtt cattgcgctg attcagcagg atctggaact
     3721 ggcaccagaa cgtattcgtc atagtgaagc gctggtgaaa aaactggtct gagtaaagaa
     3781 tgcgcgtata atgcccgccc ggtttgtgtt gttttgagag tttccttatg gcgttgttaa
     3841 tcactaaaaa atgcatcaat tgtgatatgt gtgaacccga atgcccgaat gaggcgattt
     3901 caatgggaga tcatatctac gagattaaca gcgataagtg taccgaatgc gtagggcact
     3961 acgagacacc aacctgccag aaggtgtgcc cgatccccaa tactattgtg aaagatccgg
     4021 cgcatgtcga gacagaagaa cagttgtggg ataaatttgt gctgatgcac cacgcggata
     4081 aaatttaata ttctactctg gaagtagagt attaattata ttactgggaa gccttaacgc
     4141 cattatattt atttaattga tgacattagc ataatcattc actaagttaa tttatatagt
     4201 atctgccaag acacttattt atagttatta aaggcgcgtc cgattggttc accggacgca
     4261 ccttaagtac gtttccttgt gttataagaa cagaaggatc agctgtaaaa cagcaatgat
     4321 gattttgatg acccgtttaa tcaggtatcg gcaatcagtc attcgttttt ccttaaacaa
     4381 ggaatgcagc cgttcagatt agcccttaca tctccccaaa actgaacgtg cgagttattg
     4441 agggtgcatg ctgcactcca caccagagct ttgacgacac cactcgtttc aatgggggaa
     4501 ttctgtggca tggggtaaag cacagcaaaa tcttcaataa cgaagccaat tttaatgtac
     4561 ttacgaattg gcggtcacgt taatctttcc atcaatattg ctttcttcgt aaaggctcga
     4621 gtttttatgc taaagattgc aagttgcttg taaaagataa gtacactgat ccataatcgc
     4681 tgttgttgag ggtgcatgct gcacaaaatt aaagttaaaa agtaaaaccc ccgttcctta
     4741 ccagttcggg ggttttactt tttaaagaga acggtattat tttaactttc aataattacc
     4801 gtggcacaag catagtggcg ttcatctgcc agcgttacat gcatatttac aacgccgagc
     4861 ttttccgcca gttttaatgc ctcaccccat agccgtagcc gtggtttgcc gagctcatca
     4921 ttgaatactt caaattgatt aaacgccaga ccattgcgga tcccggtgcc aaacgctttt
     4981 gctgcggctt ctttcacagc aaaacgcttc gccagaaaac gcaccggctg gtggtgcgtt
     5041 ttccagattg cccattcgtt atcgcttaat acgcggcgtg ccaggcgctc accggatcgg
     5101 gcgatcactg cttctatgcg agcgatctct acgatatccg tgcctaaacc taatattgcc
     5161 attagccacg cgcttccagc atcagacgct tcatttctgc caccgcatct ttcagtccgg
     5221 tcatcactgc acgaccaata atggcatgac cgatattcag ttcatgcatc tcagggatgg
     5281 cggcaatagc tttcacgttg tgataggtca gaccgtgacc agcgttaact ttcagaccga
     5341 gactagcggc aaaggtcgcg gctttggcga tacgcgccag ctcttgcgcc tgttcggcgt
     5401 cagttttggc atcagcatag caaccggtgt ggatctcgat aaacggtgcg ccaacctctg
     5461 ccgcagcttt gatctgctct tcatcggcgt cgataaagag agaaacctgg atcccggcat
     5521 ctgccagacg tttgcaggca tcgcgcattt tgtcacgctg ccctgcgacg tccaggccgc
     5581 cttcggttgt tacttcctga cgcttttccg gtaccagaca gcaaaaatgt ggcttcgtct
     5641 caacggcgat cgccagcatc tcttcggtca ccgccatctc cagattcatg cgggtatcca
     5701 gcgtctgacg caggatgcgc acatcacggt cagtaatgtg gcggcgatct tcacgtaaat
     5761 gcacggtaat gccgtccgct cccgcctgct cagcaataaa cgccgcctgc accggatccg
     5821 ggtaagcagt accgcgtgcg ttacgcagcg ttgcaatatg gtcaatgttg acgcctaaca
     5881 gtaattcagc catgacaatc ctcatcattc ataatgtgtt ttcaccgttc gcttaggcat
     5941 aaactgccgg aacagttccc tgctctttaa tggtttaccg ccaagatacg gcttaagcgc
     6001 catgcgggta aagcgtttcg cggcgcgtag tgtgtctgcg tcaggaaatt cccgcgcgtt
     6061 taacgctttt aactgccttc cggtgaacgt tttattgtcg ataacgacgc ttgcgataaa
     6121 ccctttttct tcgcgataac gatacgtcat ggtgtcatct accggctcgc cgctacccgc
     6181 acaatgggta aaattgacgc catagcccag atgcccgagc agtgccagtt caaagcggcg
     6241 cagcgcgggt tctggcgtac cagtggcacc tgcaagagac tgaatgcagt gcaagtaatc
     6301 gaaaaagagt tcagagaagc gcgtctcgta ttccagtacg cgggagagaa gttcgttgat
     6361 gtacagaccg ctgtaaagcg tgataccgct taatggcagc gccagcgaga cagcttcagc
     6421 actgcgcagc gttttgactt cgccacgccc gccgaaacgt agcaagagag gggtgaaagg
     6481 ctgtaatgca cctttcagag tagagcgttt agagcgtgcg cctttggcaa ccagacgcac
     6541 gcgacccgat tcctccgtga agacgtccag catcaggctg gtttcgctcc acgggcgact
     6601 atgcaggaca aatgcgcgct gccagccttc catcggagtg actcttaaag atcgtcaacg
     6661 taaccgagac tgcgcagtgc gcgttcgtcg tcggcccaac cggatttcac ttttacccac
     6721 agctcaaggt ggacaggcgc ttcgaacatt tcctgcatgt ctttacgcgc ttcaatcccg
     6781 atggttttga tcttggcccc tttgttgcca ataaccatct tcttctgccc ttcacgctca
     6841 acgagaatca aaccattgat gtcataacca ccgcgttcgt tagagacgaa acgttcgatc
     6901 tccacggtca cggagtacgg cagttcagcg ccgaggaaac gcatcagttt ttcgcggatg
     6961 atttcagacg ccataaaacg ctgtgagcga tcggtgatgt aatcttccgg gaagtgatgg
     7021 gtcgcttcag ggaggtgctt acgcacgatt gctgcgatag tgtcgacatt cagcccggtt
     7081 tcagcagaga tcggcacgat atcgaggaag ttcatctgac ttgccaggaa ctgtaagtgc
     7141 ggcagcagat cggctttttc ctgcacgtta tccactttgt tcaccgcgag gatcaccggc
     7201 gctttgccat cgcgcagttt gttgagcacc atttcgtcgt ccggcgtcca gcgggtgcct
     7261 tcaacgacaa aaatcaccag ctcaacgtcg ccaatagagc tgctcgccgc tttgttcatc
     7321 aggcggttaa tagcgcgttt ttcttccata tgcaggccag gggtatcaac gtagatcgcc
     7381 tgatacgcgc cttcagtatg gatccccaca atgcggtgac gagttgtttg cgctttgcgg
     7441 gaggtgatgg agatcttctg ccccagcagt ttgttcaaca atgtggattt gccaacgttc
     7501 ggacgtccga cgatggcaat aaatccgcag taacttttat cgatgctcat tccagctcca
     7561 gttttttcaa cgcctgttcg gcggcagcct gctcagcctt acgacggctt gaacctgtgc
     7621 caaccaccgg ttcactcagg ccgctgacct ggcagtggat agtaaattcc tgatcgtgcg
     7681 cttcgccacg tacctggact accagataag tcggcagcgg cagatggcga ccctgcaaat
     7741 attcttgcaa gcgcgttttc ggatcttttt gtttatcgcc tgggctaatt tcgtccaaac
     7801 gagtttgata ccagttgagg attaatttct cgacggtttg aatatcactg tcgaggaata
     7861 cgccaccaat taatgcttcg acggtgtcgg cgagaattga ctcacgacga aatccaccgc
     7921 ttttaagttc acctggccct aaacgtaagc actcgcctaa ctcaaattcg cgcgccagtt
     7981 ccgccagcgt attgccacgg accagcgtgg cgcgcatccg gctcatatcg ccttcatcca
     8041 cacgagggaa acggtgataa agcgcattgg cgataacgta gctcagaata gagtcgccta
     8101 aaaattctaa acgctcgtta tgtttactgc tggcactacg atgagttaat gcctgctgca
     8161 acagttcctg atgattaaaa gtgtagccca gcttccgttg aagccgatta attacgatgg
     8221 ggttcatgcg ataccaataa ataaatgcgt caacaattca gcacacgaaa cagacctgat
     8281 atacatggct ctgctaactg cttcgctgca gtttcagttt accggtatat ggggaccaac
     8341 gctgtttcgt gtgccgtggc aacctggagg tgccaacctt aaacttcggg ggaatattct
     8401 atacacaacg acgggggatg tcgttagcca cgggagattt atctcataaa taattcacgt
     8461 tgtcgccata acggcgacaa cgtgaacgaa gatgagctat taatggatgc cgccaatgcg
     8521 acttaagcgc acaccagtcg gccattcgcc ttcttgctta tcgaagctca tccagatagc
     8581 cgttgcccga ccgaccagat tcgcttcagg cacaaacccc caataacggc tgtccgcgct
     8641 gttgtcgcgg ttgtcgccca tcatgaagta ttgtcccgga ggaacaatcc aggttgccag
     8701 ttgttgccct ggctgctggt aatacatccc cacctgatcc tgcgcaatcg gcactgtcag
     8761 aattcggtgc gtcacatcac ccagtgtctc tttacgctcg gaaagacgaa ttccattttc
     8821 tttggtttcg tttttcggca cttcaaagaa tccgctggtc gcttccccac cattacggcg
     8881 tgagaaggtc tgaacgaaat cgctcggttc cacgtttgag taggtgaccg gcagcgcgtt
     8941 ttcacacgcc tggccggaac tgcatcccgg ttgaatcgtc agctctttcg agaccggatc
     9001 ataagtaact ttatcgcccg gtaaacccac cgcgcgcttg atgtaatcaa gctttggatc
     9061 ttccggatat ttaaagacca cgatatctcc gcgtttcgga tgaccggttt cgatcagcgt
     9121 tttctggtag ataggatctt taatgccata agcaaacttt tctaccagaa taaaatcacc
     9181 gattaacaga gtcggcatca tcgaacctga cgggatctgg aacggttcat aaataaacga
     9241 acgcacaatc aatacgatag ccagtaccgg aaaaacagaa gcaccggttt ccagccagcc
     9301 aggcttcggc gcaacctttt tcaacgttgc tttatccagt gagtcaccgg cagccgcctg
     9361 cgccgctgcc tgacgttccc gccgtttagg tgcgaaaaag aatttatcca cgcaccataa
     9421 aatgcccgtc accagtgtgg caatcaccag aatcagggca aacatattcg ccatgccaac
     9481 tcctaagggt tatttgttgt ctttgccgac gtgcagaatg gcgaggaacg cttcctgcgg
     9541 cagctcgacg ttaccgatct gcttcatgcg tttcttacct tctttctgct tctgcagcag
     9601 ctttttctta cggctgatat cgccgccata acatttagcc agtacgtttt tacgcagctg
     9661 tttcacggtg gatcgcgcaa taatgtgcgt accaatcgct gcctgaatgg cgatatcaaa
     9721 ctgctggcgt gggatcagat ctttcatctt ctccaccaac tcgcgaccgc ggttttgcga
     9781 attatcacgg tgggtgatca gcgccaacgc atcaacacgt tcaccgttga ttaatacgtc
     9841 tacacgtacc atgtcggacg cctggaagcg tttgaagttg taatccagag acgcataacc
     9901 acgcgaggta gatttcaggc gatcgaagaa gtcgagcacc acttccgcca tcgggatctc
     9961 gtacgtcagc gccacctgat taccgtggta aaccatattg gtctgcacgc cgcgtttttc
    10021 tacgcacagc gtaataacgt tgccgagata tgcctgcggc agcagcatgt gacattcggc
    10081 aatcggctcg cgcagttcgt agatgttatt taccgctggc agcttggatg gactgtcgac
    10141 gtaaataact tctcttgacg tggtttcaac ttcatacact acggtcggcg cagtggtgat
    10201 cagatccaga tcgtattcac gttccagacg ttcctggatg atctccatgt gcagcaggcc
    10261 gaggaagccg cagcggaaac caaagcccag cgcgctggag ctttccggct catagaacag
    10321 tgaagcatcg ttcaggctga gtttacccag cgcgtcacgg aaggcttcat agtcgtcgga
    10381 acttaccggg aacagaccgg cgtatacctg cggtttgact ttcttaaagc caggcagcgc
    10441 cttttctgcc ggattacgcg ccagcgttaa ggtatcgccg actggagcgc cgtggatatc
    10501 tttaatcgca cataccagcc agcctacttc gccacatttc agttcagtgc ggtcaacctg
    10561 tttcggcgtg aagatgccca gacggtcggc gttataggtc tgcccggtac tcatgacttt
    10621 cactttgtcg cccttacgca gggtgccgtt tttaatacgg ataagtgaaa caacgcccag
    10681 gtagttgtcg aaccatgagt cgataattag tgcctgcaac gggccttccg gatcgccttc
    10741 cggcggcgga atgtcgcgca ccagacgttc gagaacgtcc tgcacgccaa cgccggtttt
    10801 cgctgaacag cgcaccgcgt cagtggcgtc gatgccgacg atatcttcaa tttcttccgc
    10861 cacgcgttca ggatcggctg ccggcaggtc aatcttgttc agtactggca ccacttcgag
    10921 atccatttcc atggcggtgt agcagtttgc cagggtttgc gcttccacgc cctgcccggc
    10981 gtcgaccacc agcaatgcac cttcacaggc agccagcgaa cgggaaactt cataggagaa
    11041 gtctacgtgg cccggggtgt cgataaagtt aagctgatag gtttcgccgt cagacgcttt
    11101 gtagtccagc gtcacgcttt gcgctttgat ggtaatgcca cgctcacgct caagatccat
    11161 ggaatcgaga acctgcgcct ccatttcacg gtcagacagg ccaccgcaga tctggataat
    11221 acggtcagac agcgtcgatt taccgtggtc aatgtgagct atgatcgaaa agttacgtat
    11281 attcttcata aagtatgatt attgtgcctt aatgcccggt taaccaggct tttagaagtc
    11341 gctgttctga gcttaacgtc tgtattaata gaaacgccgc attctacact acaacattga
    11401 ggcgaggaaa tgttcatacc gtatggattg tggtatctgg aaacgtcctc gcatttgtta
    11461 tgcaaaatgc aacaaagcca gtgaaatcac tggctcgcgt cttccgaaga tgtttcaaat
    11521 cgcacaaggc caggcggcaa tgccacgctt aagatgatcg gttgccattc tgcccgggca
    11581 gcaaacttac gcgagtagcc gcgcgcaatc aggaacccgc caataccacc gaggatcgca
    11641 ccacataatg ctgcgacgtc agaagcaaag agtagctgaa atagcgaagc gataaggaat
    11701 aatcccacca gcggcgacat ataaaccagt aatgcggagc taagcaggct gccttcggcg
    11761 atccccaatt ccactttttg ccccggcact aacggttcat cacagggtac gacaatggta
    11821 tgcgtggttt gcgggccaag tttattcaac acgcggctac cgcaaccggc gcgtgaagcg
    11881 cagctgctgc atgaagcttt aacatcacaa ctgaccagcg cctgcccgtt ttgccaggag
    11941 acgacggtag cccactcttt gatcattgcg ctgccccgaa cttaatattc tcggcaatgc
    12001 gtttcgccgt ttgcggcggc agttcaccga caatggtgat ttcggcgtta tcacgtacgc
    12061 ttgtactgac ggttctgcgt ccggtgcgca acatctgatc ggtgctcgat ggcgtagcgc
    12121 ggttaacgtt taccgagaag ctgaataatc cgtcggaata gagacgtgat tcgataggca
    12181 tgttgtccat cgtcggtagc ggacgtcgac tactggaaac ttcgctaaaa ccctgtggca
    12241 accaggttgg cgtccagctg aatttagctt tttcacctac aggaacagaa agcaacggcg
    12301 gcaaatttgc cttcgccagc gtctgcatac tgctactgat atcctgattg acgttaaaag
    12361 caatcacgcg aaattgttcc agcgtttcac catcgcgatc aaggagatca accctcatcg
    12421 gtaatttcga ttcgatgtcc atccacacga tgtagctgta gcgtgtacca tctcgggcaa
    12481 ccacgcgaat gacttcgcaa agacgatcag caatacgcgt gcgcccgacg gagataaagt
    12541 cgtagtaagg agaaaggcgt ttgaaatcgg tatagatgag cgatggcaga gaatcaacaa
    12601 tgtaatcgcc attaagcgtg aacggttcaa gtcccggttc aaaatagctg atttcattgc
    12661 cacgctgtac cacttcccgg cgcgggccat ccatttgcaa caattgtgca agaggacggt
    12721 tatcgaggcg tgcatgtcga taacgcagag actcaacacc ctgtttattg atgctgatga
    12781 atgacagctc gtaattcagt gactgactgg ccaggttcat ctgctgtaat aacgccccgg
    12841 acgcgggagt ggccgaggcg ttagcagaga ataacaggct acctgtcact aatgacatgg
    12901 caaaccaaag ttgcttcatt actgcgattg cgttcctaaa gtttgaattc ctggcacctg
    12961 tacagcggct tgctgggttt gcgcctgctc aaactgaagc tgttcagagt ggagtcggcg
    13021 ttgcagttcg taatcctgca acattgcatt aatgcgacga cgctgctcct gtacctgctg
    13081 ctgttgacca ttgtttgcgg tcgcttcaga aggtactccc aggcttaccg ggctggcttt
    13141 acccatcatc ggcagtgtat taaataccgg cgtttcgggc tgctgggacg tttcagattg
    13201 tccattatag tgctggacgc caacgataac tgcaagcgat acgcatgctg ctacgcccat
    13261 ttgggtaagc tgtgccgccc acggacgtac tttctgccag aatggcattt tctgccattg
    13321 atgcggcgca ggctgggctt ccgggatcaa tgtcgccggt tgacgtactg gctcttcttc
    13381 aatggcggcc atcacgcgtg aagagatatc gaaatggagc acctcgggag tatcaccccg
    13441 cattgagtca cggattaagt gatagctttc ccaggttttc tgcatttctg ggttatgagc
    13501 cagttcgtta agcagctcac tatccagcgt ttcgccatcc attaaagcgg aaagttgttc
    13561 tttctgcatg cctaataccc ttatccagta tcccgctatc gtcaacgcct gataagcggt
    13621 tgaactttgt tatcaatagc ttccctcgct cggaagatac gtgaacgcac cgtacctacc
    13681 ggacaatcca tgatagcggc tatctcttca tagctcaggc catccagctc ccgcaaggtt
    13741 attgccatgc gcaaatcttc cgggagggac tcaatagttc ggaaaactat ctgtctcagt
    13801 tcttctgaca acattaagtt ctcagggttc gaaatttctt tcaacgcgcc gccactttcg
    13861 aagttttcag cttcaatggc atccacatca ctggaaggtg gacgacgccc ctgagcaacc
    13921 aggtaatttt tcgctgtatt tacagcaatc cgatacagcc atgtataaaa agcgctatct
    13981 ccccggaacg aatccagcgc acgataggct ttaataaaag cttcttgtac cacatcggga
    14041 acatcacccg acggcacata gcgggaaacc agactcgcca ctttatgctg atagcgcact
    14101 accagtaggt taaaggcttt ctgatctccc ttctggaccc gttcaaccag gacctggtcc
    14161 gttaactgct cgctcatccg aggtaaagtc tccccaaacc aaatttccac gcgctatcga
    14221 aacgccactc cattagctgc aatttgagca agcaaagggt tagagtgtct cgtttttgta
    14281 aagttccgta acgcatctgt ttttgtttgt catgctgtag acggatcatt atctatcatt
    14341 ataagtctac agaatctgaa catcgcatta tctgtgtaga aatgcccatt taactgcctg
    14401 aagagtaacc caacggcctt tttatttcac cacctaatcc tccaccagcc agtaacttct
    14461 ctttttctcg ccgccctgcg tcagcgtgtt tagccactgt aacaaatatt aaaatagcag
    14521 gtgtttattc gcacaacatg atgctatgct gaccaaacgg tgtttagtaa attaaacaaa
    14581 gaaaatgaat actctcccag aacattcatg tgacgtgttg attatcggta gcggcgcagc
    14641 cggactttca ctggcgctac gcctggctga ccagcatcag gtcatcgttc taagtaaagg
    14701 cccggtaacg gaaggttcaa cattttatgc ccagggcggt attgccgccg tgtttgatga
    14761 aactgacagc attgactcgc atgtggaaga cacattgatt gccggggctg gtatttgcga
    14821 tcgccatgca gttgaatttg tcgccagcaa tgcacgatcc tgtgtgcaat ggttaattga
    14881 ccaaggggtt ttgtttgata cccacgttca accgaatggc gaagaaagct accatctgac
    14941 ccgtgaaggt ggacatagtc accgtcgtat tcttcatgcc gccgacgcca ccggtagaga
    15001 agtacaatcc acgctggtga gcaaggcgca gaaccatccg aatattcgtg tgctggagcg
    15061 cagcaacgca gttgatctga ttgtttctga caaaattggc ctcccgggca cgcgacgggt
    15121 tgttggcgcg tgggtatgga accgaaataa agaaacggtg gaaacctgcc acgcaaaagc
    15181 agtggtactg gcaaccggcg gtgcgtcgaa ggtttatcag tacaccacca atccggatat
    15241 ttcttctggc gatggcattg ctatggcgtg gcgcgcaggc tgtcgggttg ccaatctcga
    15301 atttaatcag ttccacccta ccgcactgta tcacccacag gcacgcaatt tcctgttaac
    15361 ggaagcactg cgcggcgaag gcgcttatct caagcgcccg gatggcacgc gttttatgcc
    15421 cgattttgat gagcgcggcg aactggcccc gcgcgatatt gtcgcccgcg ccattgacca
    15481 tgaaatgaaa cgcctcggcg cagattgtat gttcctcgac atcagccata agcccgccga
    15541 ttttattcgc cagcatttcc cgatgattta tgaaaaattg ctcgggctgg ggattgatct
    15601 cacacaagaa ccggtaccga ttgtgcctgc tgcacattat acctgcggtg gtgtaatggt
    15661 tgatgatcat gggcgtacgg acgtcgaggg cttgtatgcc attggcgagg tgagttatac
    15721 cggcttacac ggcgctaacc gcatggcctc gaattcattg ctggagtgtc tggtctatgg
    15781 ctggtcggcg gcagaagata tcaccagacg tatgcctgat gcccacggcg tcagtacgtt
    15841 accgccgtgg gatgaaagcc gcgttgagaa ccctgacgaa cgggtagtaa ttcagcataa
    15901 ctggcacgag ctacggctgt ttatgtggga ttacgttggc attgtgcgca caacgaagcg
    15961 cctggaacgc gccctgcggc ggataaccat gctccaacaa gaaatagacg aatattacgc
    16021 ccatttccgc gtctcaaata atttgctgga gctgcgtaat ctggtacagg ttgccgagtt
    16081 gattgttcgc tgcgcgatga tgcgcaaaga aagccggggg ctgcatttta cgctggatta
    16141 cccggaactg ctcacgcatt ccggtccatc gatcctttcc cccggcaatc attacataaa
    16201 cagataaaaa gcctgggtca gcgccgtata cgcttcggaa tagttctggt ctggcccacg
    16261 aatgactaag cgatcgctaa agcattctcc cgcctgcggg gagaatgcca gcagcacccg
    16321 atgcggcagt cgcgcttcgt tttccgccac atccgtccgc aaacgtaaat gccagcccat
    16381 gcttaatgcc agttcagtaa aaccattacc aatctgctct ggcagcacca cgcagaaaaa
    16441 tccctcttcg gtaatgcact ccgccgcaca ggtcagcagc gatgggtgat caagcgtagt
    16501 ggtatagcga gcctgttccc gttgaggtgt cgcgcactct actccctgct gatagtaagg
    16561 tgggttactg atgattaaat cgaagcgtac tgtctgctgt gtgatccact gctgaatatc
    16621 cgccgtatgg acgttaatcc gctctgccca cggggactgg ttgatatttt cctgcgcctg
    16681 cgcggcagct tcactttcca gctcaacggc atcaattatc acgctgtcac tggttcgctg
    16741 cgccagcatc agtgccagca acccgctacc cgcgccgata tcaaggcaac gtttcacccc
    16801 agccaccggt gcccatgcgc ccagcaaaat gccatccgtt cccactttca tcgcacagcg
    16861 atcgtgagca acaaaaaact gtttaaaagt aaatccatta cgacgaagca cggatgtaga
    16921 ctgtgacatg aaaataaaac cttgcaggaa aaacggcgat agcaccgggt gagaacaata
    16981 cccgagaagc gatatccata caaacagatg aagattgcag ccgtaacgtc tataatcagc
    17041 gccccacaca gaggtagaac atgactgtaa cgactttttc cgaacttgaa ctcgacgaaa
    17101 gcctgctgga agccctccag gataaaggtt tcactcgccc gaccgccatt caggctgccg
    17161 ccattccgcc tgcgctcgat ggccgtgatg tactcggttc tgcgccgaca ggcaccggta
    17221 aaacggcggc gtatctgctg ccagcgttgc agcacctgct cgatttcccg cgtaagaaat
    17281 ccggtccgcc gcgtattttg atcctcaccc caactcgcga gctggcgatg caggtggccg
    17341 atcatgcccg cgaactggcg aaacatacgc atctggatat cgccaccatc accggcggcg
    17401 tagcctatat gaaccacgcg gaagtgttca gcgaaaatca ggacatcgtg gtcgccacga
    17461 ccggacgtct gctgcaatac ataaaagaag agaacttcga ttgccgcgcg gttgaaacgc
    17521 tgatcctcga cgaagcagac cgtatgctgg atatgggctt cgctcaggat atcgaacata
    17581 ttgctggcga aacgcgctgg cgtaaacaga ccctgctctt ttcggcaacg ctggaaggcg
    17641 atgcgattca ggactttgcc gagcgtctgc tggaagatcc ggtggaggtt tctgccaatc
    17701 cgtccacccg tgagcgcaaa aaaattcatc agtggtatta ccgcgccgat gacctggaac
    17761 ataaaaccgc attgctggtg catctgttaa aacagccgga agcgacccgc tcaattgtgt
    17821 ttgtgcgtaa gcgtgaacgt gtgcatgagc tggcaaactg gctgcgcgaa gcgggcatca
    17881 acaactgcta tctcgaaggt gagatggtac aaggcaagcg taacgaagcg atcaagcgtt
    17941 tgaccgaagg tcgcgtaaac gtactggttg caaccgatgt tgccgcgcgc ggtatcgaca
    18001 ttcctgacgt cagccacgtc tttaacttcg atatgccgcg cagtggcgat acttatttgc
    18061 accgtatcgg acgtaccgcg cgcgccggtc gtaaaggcac cgcaatttcg ctggtggaag
    18121 cccatgacca tttgctgctg ggcaaggttg gccgttacat tgaagagccg attaaagctc
    18181 gcgttattga tgagttacgc ccgaaaacgc gtgcgccaag cgagaagcag accggcaagc
    18241 catcgaagaa agtactggca aaacgcgccg agaagaaaaa agctaaagag aaagagaaac
    18301 cgcgtgtgaa aaaacgccat cgcgacacca aaaatattgg taagcgccgt aaaccaagcg
    18361 gaacgggcgt gccaccgcaa acgacagaag agtaatctca atgccgggtt taagcctggt
    18421 attaaaagtg ctggcaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    18481 ctcatgcaat atattgaatt tgcacgatct tgtaggccgg ataaggcgtt cacgccgcat
    18541 ccggcatgaa taaagcgcac tttgtcgaca agttaaatgc cgggtttaat cccggcatta
    18601 tttttatcca gccacaaaac actcttcaac acactgaata aaagtgtgca tcgccggact
    18661 aaccgctttt ccggcatggt gagcgcacat tgccgtaatg gtctgcgtct gttcgccaaa
    18721 aggcaattcg attaattcgc cactcgcaag ttcatttccc actgcaaagc gcggcaggta
    18781 actgaccccg atatttgccg cgacacaacg cttgatgctt tcgatactta taagctcaat
    18841 ggtgttttcc accgtgatcc gccgctggcg cagcgtgctc tcaaatatct gccggaagac
    18901 acattgtggt tcgttgataa taaagctaca ggcgttatgt cttcccggct cagtaaaatc
    18961 gatatctgca atttgcggtg aagccaccag caccagcggt tgttcaccca actctcgtcg
    19021 gttcagaaca tcatcattcc ctacgcgata aaaaaccccg acatcggcct catcattcag
    19081 tagcgcatca cggatcacgt aacagttcag cgactgcaac gataaacgca cttttggagc
    19141 gcgttgccga aaacgctgca acacctgcgg cattcggtaa gagagcaacg tttcgcctga
    19201 aacaacgcgt agttccccgt ccggatccga ctcttttttc gcagcctcac gaagcgtatc
    19261 catcactcgg gtaagttcat agatgtgcgg cagcaacttt tttccttcgc gggtaaggca
    19321 cattcgtcga ccaattttct caaacaattg taccgagaac tcctgctcaa gctgctgaat
    19381 atgaaaagtc accgtcgatt gtgtacagca caatttttgc gaagctcgca aaaaggaacc
    19441 ctcttccacc acggttttaa gtgtaataaa acggcgcaga tccataaccc caaacctatc
    19501 gaaaatatcg aatttagaat gtaaaaacat tcattttttt aaatgttccg tgtcgggtac
    19561 tgtctaccaa aacagaggag ataacaagtg acaccgaccc ttttaagtgc tttttggact
    19621 tacaccctga ttaccgccat gacgccagga ccgaacaata ttctcgccct tagctctgct
    19681 acgtcgcatg gatttcgtca aagtacccgc gtgctggcag ggatgagtct gggatttttg
    19741 attgtgatgt tactgtgtgc gggcatttca ttttcactgg cagtgattga cccggcagcg
    19801 gtacaccttt tgagttgggc gggggcggca tatattgtct ggctggcgtg gaaaatcgcc
    19861 accagcccaa caaaggaaga cggacttcag gcaaaaccaa tcagcttttg ggccagcttt
    19921 gctttgcagt ttgtgaacgt caaaatcatt ttgtacggtg ttacggcact gtcgacgttt
    19981 gttctgccgc aaacacaggc gctaagctgg gtagttggcg tcagcgtttt gctggcgatg
    20041 attgggacgt ttggtaatgt gtgctgggcg ctggcggggc atctgtttca gcgattgttt
    20101 cgccagtatg gtcgccagtt aaatatcgtg ctcgcgctgc tgctgatcta ttgcgcggta
    20161 cgcattttct attaattaaa aaagcggaag aggtcgccct cttccgctta gtaacttgct
    20221 acttaagcct tacaggcttt cagtaaaggt acgagcgata acgtcgcgct gctgttccgg
    20281 agtcagagag ttaaagcgaa ctgcataacc tgatacacgg atggtcagct gcggatattt
    20341 ttccggatgc ttaactgcat cttccagagt ttcacgacgc agaacgttaa cgttcaggtg
    20401 ttgaccacct tcaacgcgaa cttctggttt cacttctact ggaacttcac ggtattcaat
    20461 gtcacccagt ttgcttactg caaccacttc atcttctgca taacctgctt ttgcaacgat
    20521 gcaacgcgct tcgccttttt cgctgtccag cagccagaaa gagttcagca gatcgtcgtt
    20581 agcggcttta gtaatctgga tacctgtaat catgtgatgc ctccccggca aaattatttg
    20641 atttattcag cctgtcgcgg ccaattggta aaaccattgt tgcttgagtg tatatatact
    20701 cctcaacaac ccttgattcc ttgatttaaa tcaataaaaa ccacacatcg attgtggtcg
    20761 caaatggatt ttattgtttt acatcaactt atgcgggtgt gaaattttac caatttacat
    20821 ttttttgcac tcgtttaagt ctaaaaaatg agcatgattt tgttctgtag aaataagcag
    20881 ttaagctagg cggattgaag attcgcagga gagcgagatg gctaacgaat taacctggca
    20941 tgacgtgctg gctgaagaga agcagcaacc ctattttctt aatacccttc agaccgtcgc
    21001 cagcgagcgg cagtccggcg tcactatcta tccaccacaa aaagatgtct ttaacgcgtt
    21061 ccgctttacc gagttgggtg acgttaaagt ggtgattctc ggccaggatc cttatcacgg
    21121 accgggacag gcgcatggtc tggcattttc cgttcgtccc ggcattgcca ttcctccgtc
    21181 attattgaat atgtataaag agctggaaaa tactattccg ggcttcaccc gccctaatca
    21241 tggctatctc gaaagctggg cgcgtcaggg cgttctgctg ctcaatactg tgttgacggt
    21301 tcgcgcaggt caggcgcatt cccacgccag cctcggttgg gagacgttta ctgataaagt
    21361 catcagcctg attaaccagc accgcgaagg cgtggtgttt ttgttgtggg gatcacatgc
    21421 gcaaaagaaa ggggcgatta tagataagca acgccatcat gtactgaaag caccgcatcc
    21481 gtcgccgctt tcggcgcatc gtggattctt tggctgcaac cattttgtgc tggcaaatca
    21541 gtggctggaa caacgtggcg agacgccgat tgactggatg ccagtattac cggcagagag
    21601 tgagtaaatt tgcggggaaa tgccggatgg cagagttgcc acccggctga tttatcaggc
    21661 tttattctga cgccaccatt caccaagcaa aacgccggtt gcgacagaga tattcagccc
    21721 ggcaacgttg cccgtaccgt caatcttcac gcgcagatcg ttcggatcgc gtgcggcatc
    21781 cggtaaccct tcatattcct gacccagcac cagtaccatt ttcgctggca gactagtttt
    21841 gaacagcggt ttaccctgct cgctggaagt ggtcactacg gtgtaacctg cctgacggaa
    21901 atcatccagc acgttaacaa tgttgtcgcc agtaatcggc tgaacgtgct ctgcgccgcc
    21961 ttctgcggta cggatagccg cccccgactc cagcagtgcc gcatcctgca ccacgacacc
    22021 tttcacgccg aagtgcgcgc agctgcgcat catgccaccc aggttatgcg ggttagattc
    22081 gttttccagt gccagaacgc aatcctgcgc gcctgcctgg cttacccact gctgcacggt
    22141 tgtaccgtta cgctttttga tcaagaagca aacgccgccg tgatgttccg tgcctgacgc
    22201 ttttgtcaat tccgcttcat ccaccacatg gtacgctttg cggtttgctg ccatccagcg
    22261 caaggcttct ttaaaacgcg gcgttacact ctggataaac caggcgcgaa caatcgcttc
    22321 cggacggctc tggaaaagtg cctgacatgc attttcgccg tagacgcggg tttcttccgc
    22381 acgctgacga cgcaacactt ccggatcaat aaaactttta ccactgatgc caccgtgatc
    22441 ggccttttcc ggcgtctcat cacccggcgc gcgggaaacc gtgcgccacg gcgaatcttc
    22501 ccacttgcgg tcacggggct gactctgttt gtcatcgcgg gcggggcgac ggccaccgtc
    22561 agcacgagat tttcctggac gcccgccccc tttcccggta cgcgggttgt gggtacgttt
    22621 atcagaatca tcatcactgc ggacatacat cactttgacc ttgccgcttt tacctttcat
    22681 ttcatcgttc atgcttttct ccaccagcgc tgcgcgaagc gcgcagatta cccgaagtcc
    22741 gcgcggttcg ccatgatttc gtaccaaagc ctgcgactat catacctatt gaataaaaca
    22801 gattgttgtc tggaacaatg tccccgataa tatgtaacat attagaaaca taccggcgtc
    22861 gttgccgata agtctcctta ctcatcccga ggttagttat gaataccgtt tgtacccatt
    22921 gtcaggccat caatcgcatt cccgacgatc ggatcgaaga tgcggcaaaa tgcggacgct
    22981 gcggtcacga cttgtttgac ggagaggtga ttaatgcgac cggtgaaaca ctcgacaaat
    23041 tgctgaagga tgatctacct gtggtgatcg acttctgggc accgtggtgc ggcccctgcc
    23101 gtaatttcgc accaattttt gaagatgtcg cgcaagagcg tagcggtaaa gtgcgctttg
    23161 tgaaagtgaa taccgaagct gaacgtgaat tgagcagtcg ctttggaatt cgtagtatac
    23221 cgacgatcat gattttcaaa aacggtcagg ttgtcgacat gcttaatggc gcagtaccga
    23281 aagcgccgtt cgatagctgg ctgaacgaat ctctttaatc ttaccggggc acatcttgtg
    23341 ccccgttttc tcctctgcga caatggcgtt ttttcgacgc tctcttatga ccgaaaacgc
    23401 tgttctccag ttacgcgccg agcgtattgc gcgcgcaaca cgtccttttc ttgcccgcgg
    23461 taatcgcgtt cgtcgctgcc aacgctgtct tttgccagag aaattatgtc tctgttcgac
    23521 aattacccca gcacaagcaa aaagtcgctt ctgtttgctg atgttcgaca ccgagccaat
    23581 gaagccaagt aacaccgggc gtctgattgc tgatattttg cctgataccg ttgcgtttca
    23641 atggtcgcgt accgacccct cgcaagactt gctggatctg gtacaaaacc cggactatca
    23701 accaatggtg gtctttcccg cctcgtatgc tgatgagcaa cgggaagtga tcttcacacc
    23761 acctgccggt aagccaccgc tgtttatcat gctcgatggt acctggccgg aagcgcgcaa
    23821 gatgtttcgt aaaagtccgt atctggataa tcttcccgtc atttccgtcg atctttcccg
    23881 gctttctgcc tatcgcctgc gtgaagccca ggctgaaggc caatattgta ctgccgaggt
    23941 agccatcgca ctgttagata tggccggcga taccggggcg gcagcagggt taggcgagct
    24001 ttttacccgc tttaaaacac gctatctggc aggaaaaacg caacatctgg gtagcatcac
    24061 agcagaacag ttagaaagcg tttaaaatca ttcggtcact tctgcgggag accggtatga
    24121 gtcagcgagg actggaagca ctactgcgac caaaatcgat agcggtaatt ggcgcatcga
    24181 tgaaacccaa tcgcgcaggt tacctgatga tgcgtaacct gctggcggga ggctttaacg
    24241 gaccggtact tccggtgacg ccagcctgga aagcggtgtt gggtgtgtta gcctggccgg
    24301 atattgccag cttgcccttt acacccgacc ttgcggtttt atgtaccaat gccagccgta
    24361 atcttgctct tctggaagag ctcggcgaga aaggctgtaa aacctgcatt attctttccg
    24421 ccccggcatc gcaacatgaa gatctccgcg cctgcgccct gcgccataac atgcgcctgc
    24481 taggaccaaa cagtctgggt ttactggctc cctggcaagg tctgaatgcc agcttttcgc
    24541 ctgtgccgat taaacgcggc aagctggcgt ttatttcaca atcggctgcc gtctccaaca
    24601 ccatcctcga ctgggcgcaa cagcgtgaga tgggcttttc ctactttatt gcgctcggcg
    24661 acagcctgga tatcgacgtt gatgaattgc tcgactatct tgcgcgcgac agtaaaacca
    24721 gcgccatcct gctctatctc gaacagttaa gcgacgcgcg acgctttgtt tcggcggccc
    24781 gtagtgcctc gcgtaataaa ccgattctgg tgattaaaag cggacgtagc ccggcggcac
    24841 agcgactgct caacacgacg gcaggaatgg atccggcgtg ggatgcggct attcagcgtg
    24901 ccggtttgtt gcgggtacag gacacccacg agctgttttc ggcggtggaa acccttagcc
    24961 atatgcgccc gctgcgtggc gaccggttga tgattatcag caacggtgct gcgcctgccg
    25021 cgctggcgct ggatgcctta tggtcacaca atggcaagct ggcaacgcta agcgaagaaa
    25081 cctgccagaa actgcgcgat gcactgcctg gacatgtggc aatctctaac ccgctcgatc
    25141 tgcgcgatga cgccagcagc gagcattatg tcaaaacgct ggacattctg ctccacagcc
    25201 aggattttga tgcgctgatg gttattcatt cacccagcgc agcggctccc gcaacagaaa
    25261 gcgcgcaaga attaattgaa gcggtaaagc atcatccccg cagcaaatat gtctctttgc
    25321 tgacgaactg gtgcggcgag cactcctcgc aagaggcacg acgtttattc agcgaagccg
    25381 ggctgccgac ctatcgcacg ccggaaggca ccatcaccgc gtttatgcat atggtggagt
    25441 accggcgtaa tcagaagcaa ctacgcgaaa cgccggcgtt gcccagcaat ctgacttcca
    25501 ataccgcaga agcgcatctt ctgttgcaac aggcgattgc cgaaggggct acgtcgctcg
    25561 atacccatga agttcagcct atcctgcaag cgtatggcat gaacacgctc cccacctgga
    25621 ttgccagcga tagcaccgaa gcggtgcata ttgccgaaca gattggttat ccggtggcgc
    25681 tgaaattgcg ttcgccggat attccacata aatcggaagt tcagggcgta atgctgtacc
    25741 tgcgtacagc caatgaagtc cagcaagcgg caaacgctat tttcgatcgc gtaaaaatgg
    25801 catggccgca ggcgcggatc cacggcctgt tggtgcaaag tatggctaac cgggctggcg
    25861 ctcaggagtt gcgggttgtg gttgagcacg atccggtttt cgggccgttg atcatgctgg
    25921 gtgagggagg tgtggagtgg cgtcctgaag atcaagccgt cgtcgcactg ccgccgctga
    25981 acatgaacct ggcccgctat ctggttattc aggggatcaa aagtaaaaag attcgtgcgc
    26041 gcagtgcgct acgcccattg gatgttgcag gcttgagcca gcttctggtg caggtttcca
    26101 acctgattgt cgattgcccg gaaattcagc gtctggatat tcatcctttg ctggcttctg
    26161 gcagtgaatt taccgcgctg gatgtcacgc tggatatcgc gccgtttgaa ggcgataacg
    26221 agagtcggct ggcagtgcgc ccttatccgc atcagctgga agaatgggta gaattgaaaa
    26281 acggtgaacg ctgcttgttc cgcccgattt tgccagaaga tgagccacaa cttcagcaat
    26341 tcatttcgcg agtcaccaaa gaagatcttt attaccgcta ctttagcgag atcaacgaat
    26401 ttactcatga agatttagcc aacatgacgc agatcgacta cgatcgggaa atggcgtttg
    26461 tagcggtacg acgtattgat caaacggaag agatcctcgg cgtcacgcgt gcgatctccg
    26521 accctgataa catcgatgcc gaatttgccg tgctggttcg ctcggatctc aaagggttag
    26581 gcttaggtcg acgcttaatg gaaaagttga ttacctatac gcgagatcac ggactacaac
    26641 gtctgaatgg tattacgatg ccaaacaatc gtggcatggt ggcgctagcc cgcaagctcg
    26701 ggtttaacgt tgatatccag ctcgaagagg ggatcgttgg gcttacgcta aatcttgccc
    26761 aacgcgagga atcatgagta aggtactgga aatgttgacc acttaatcgg gactggtgtt
    26821 attattgccc gcttatgtcg tctgcattgc acagaggacc cttcaatgaa cagagaagaa
    26881 atgcactgtg atgttgtcaa aatttaagcg taataaacat caacaacacc ttgcccaact
    26941 acccaagatt tctcaatcag ttgatgatgt cgatttcttt tacgctcccg ccgacttccg
    27001 ggagacgctg ctggaaaaaa tagccagcgc gaagcagcgc atttgcattg tcgccctgta
    27061 tctcgaacag gatgacggtg gcaaaggcat tctgaacgcg ttgtatgaag ctaaaaggca
    27121 gcgtccggaa ctggatgtgc gggtgctggt cgactggcat cgtgcacaac gtggacgcat
    27181 tggcgctgcg gcatctaaca ctaacgctga ctggtactgc cgcatggcgc aggaaaatcc
    27241 gggcgtagat gttccggttt atggcattcc aatcaatacc cgtgaagccc ttggtgttct
    27301 gcactttaaa ggctttatca tcgacgatag cgtactttat agcggtgcca gcctgaacga
    27361 tgtttacctg catcagcacg ataaatatcg ctacgaccgt tatcatctta tccgtaaccg
    27421 taagatgtca gacattatgt ttgaatgggt tacacagaat attatgaatg gccgcggcgt
    27481 taatcgtctg gatgatgtta atcggccaaa aagcccggaa atcaagaacg atattcgtct
    27541 gttccgccag gagctgcgtg atgccgctta tcatttccag ggcgatgccg acaacgatca
    27601 gctttccgta acgccgctag tggggctggg gaaatcgagt ctgttgaaca agaccatttt
    27661 ccatcttatg ccttgtgccg agcagaaact aaccatctgt acgccatact tcaacctgcc
    27721 agcaatcctt gtgcgcaata ttatccagtt gctgcgcgaa gggaaaaagg tcgaaattat
    27781 tgttggtgat aaaaccgcga acgacttcta cattccggaa gatgaacctt tcaagataat
    27841 tggcgcattg ccttatctct atgagatcaa tctgcgtcgt ttcctgagcc gtttgcagta
    27901 ttacgtcaat actgaccagc tagtggttcg gttatggaaa gatgacgaca acacctatca
    27961 cctaaaaggg atgtgggttg atgataagtg gatgttgatc accggtaata acctgaaccc
    28021 gcgcgcctgg cgtctggatc tggaaaacgc cattttgatc cacgatccgc aacttgagct
    28081 ggcgccacag cgagagaaag aactggagct gatccgcgag cataccacca tcgttaagca
    28141 ctatcgcgat ctgcaaagta ttgccgatta tccggtgaag gttcgtaaac tcatccgccg
    28201 tttgcgccgt atccgcatcg accgattaat tagccgcatc ctgtaatcac aaccccgtcc
    28261 tgtacggggt ttgttttttg gaggccacgt tttgcgtatt ctttttgtct gttcgctatt
    28321 gttactttct ggatgcagcc atatggctaa cgatagctgg agcgggcagg ataaagctca
    28381 acactttatc gcctcggcga tgctttccgc cgccggaaat gaatattcac agcatcaggg
    28441 gatgagccgg gatcgcagtg ccatgtttgg attgatgttc tctgtcagtt tgggggcgtc
    28501 aaaagagctt tgggatagcc gccccgaagg gagcggctgg agctggaagg atttggcctg
    28561 ggatgtcgcc ggtgcaagca ccggctatac cgtctggcaa ctgacccgtc actaaagacg
    28621 catccccttc cctttgcgat gtagcatcaa agaaaccaga aacgccacca cggccatcaa
    28681 ggtcacatac cagaagaagg ctgtttccat tcctattgat ttcagcgaca acgctacgta
    28741 ctccgccgaa ccaccaaata tagcattagc gaccgcatat gacaggccaa cgcctaatgc
    28801 gcgaacctgt gccgggaaca tctcagcctt cagtattcca ctgattgatg tataaaaact
    28861 cactatcagc agggcacaca tcaccagacc aaaagcggca taaggcgagg aaacgttttg
    28921 caatgctgag agaataggaa cggtaaaaat ggctgccagc gaaccgaaac ataacattga
    28981 ggtacggcga ccaatcttat ccgacagcgc gccaatgagt ggttgaataa gcatgaatac
    29041 aaacaatgcg gcagtcataa tgccactcgc cacgttggca tgcattcccg cagtatttac
    29101 cagatacttc tgcatataag tggtgaaggt atagaaacaa agggagcccg cagcggtaaa
    29161 accgagaacc atgatgaatg cacggcgatt gcgccataat cctttcagag atccagcttc
    29221 ttttaaagcg cgcgtttctt gttgcgaagt ttcatctaac tgacgacgta accacaacgc
    29281 cacaacagct aacacagctc ctaacgcgaa aggaatacgc catccccact ctctgagtgc
    29341 agcgtcttcc atggtgtgtt gtaaaaccac gacaaccagt agggctagca gttgtccgcc
    29401 gatcaacgtc acatactgaa atgatgcgta aaaacctttg cgcccttcaa cggcaacttc
    29461 actcatatag gtggcgctgg tgccatattc tccgccaaca gataatccct gaaataaacg
    29521 cgcgagaagc aataatgccg gagcccacgt acctatagtt tcataacctg ggaggcaggc
    29581 gataaccagc gatccgaaac acatcataca caccgataac agcatcgatt ttttgcgacc
    29641 atgtttatcg gctatgcggc caaatagcca accgcctatt gggcgcatca ggaatcccgc
    29701 agcaaaaaca cctgctgttt gtagtagttg agtcgtcgtg ttcccggaag ggaagaaaat
    29761 gtgggcaaag tagagtgaac agaacgagta gacatagaaa tcgaaccact cgaccagatt
    29821 acctgaagag gcccccacaa tcgcccaaat gcggcgacga gtatcactac ttgtcagttt
    29881 gctgtctgcc gttacagtac tttcagccat gccattatgt ctcctgccgt aatccgatgc
    29941 ttttgtcggt cgcttttatt tatttctttt ggtaaagaaa atgttataca tttgttgcgt
    30001 atcattatgc aaccttaacc atgaatttag ttagcaggaa aatggttatt gaggagctta
    30061 aggataaatt tctggtaagg aggacacgta tggaagtagg caagttgggg aaaccgcatc
    30121 cgttgctgaa tctggcatat gtgggagtat aagacgcgca gcgtcgcatc aggcattttt
    30181 ttctgc
//
LOCUS       DAPGWD010000043        29847 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPGWD010000043 DAPGWD010000000
VERSION     DAPGWD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.043"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..1321
                     /gene="uraA"
                     /locus_tag="RHX88_001873"
     CDS             32..1321
                     /gene="uraA"
                     /locus_tag="RHX88_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDV2230568.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            1371..2117
                     /gene="hda"
                     /locus_tag="RHX88_001874"
     CDS             1371..2117
                     /gene="hda"
                     /locus_tag="RHX88_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDV2230569.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(2255..2614)
                     /gene="arsC"
                     /locus_tag="RHX88_001875"
     CDS             complement(2255..2614)
                     /gene="arsC"
                     /locus_tag="RHX88_001875"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDV2230570.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2635..4098)
                     /gene="bepA"
                     /locus_tag="RHX88_001876"
     CDS             complement(2635..4098)
                     /gene="bepA"
                     /locus_tag="RHX88_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDV2230571.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4311..5372
                     /locus_tag="RHX88_001877"
     CDS             4311..5372
                     /locus_tag="RHX88_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV2230572.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFTVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(5410..6258)
                     /locus_tag="RHX88_001878"
     CDS             complement(5410..6258)
                     /locus_tag="RHX88_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDV2230573.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VIGWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(6280..8292)
                     /gene="hyfR"
                     /locus_tag="RHX88_001879"
     CDS             complement(6280..8292)
                     /gene="hyfR"
                     /locus_tag="RHX88_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDV2230574.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(8322..8735)
                     /gene="hyfJ"
                     /locus_tag="RHX88_001880"
     CDS             complement(8322..8735)
                     /gene="hyfJ"
                     /locus_tag="RHX88_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDV2230575.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMNFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(8728..9485)
                     /gene="hyfI"
                     /locus_tag="RHX88_001881"
                     /pseudo
     CDS             complement(8728..9485)
                     /gene="hyfI"
                     /locus_tag="RHX88_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(9482..10027)
                     /gene="hyfH"
                     /locus_tag="RHX88_001882"
     CDS             complement(9482..10027)
                     /gene="hyfH"
                     /locus_tag="RHX88_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDV2230576.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFEPTVTN
                     KADLYTRATFHLQRCSRCERPFAQQKTVALAAELLAEQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(10037..11752)
                     /gene="hyfG"
                     /locus_tag="RHX88_001883"
     CDS             complement(10037..11752)
                     /gene="hyfG"
                     /locus_tag="RHX88_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDV2230577.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRMMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(11742..13322)
                     /gene="hyfF"
                     /locus_tag="RHX88_001884"
     CDS             complement(11742..13322)
                     /gene="hyfF"
                     /locus_tag="RHX88_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDV2230578.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLI
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGNLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(13327..13977)
                     /gene="hyfE"
                     /locus_tag="RHX88_001885"
     CDS             complement(13327..13977)
                     /gene="hyfE"
                     /locus_tag="RHX88_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDV2230579.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(13989..15304)
                     /gene="hyfD"
                     /locus_tag="RHX88_001886"
                     /pseudo
     CDS             complement(13989..15304)
                     /gene="hyfD"
                     /locus_tag="RHX88_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(15316..15426)
                     /locus_tag="RHX88_001887"
     CDS             complement(15316..15426)
                     /locus_tag="RHX88_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HDV2230580.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(15443..16390)
                     /gene="hyfC"
                     /locus_tag="RHX88_001888"
     CDS             complement(15443..16390)
                     /gene="hyfC"
                     /locus_tag="RHX88_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDV2230581.1"
                     /translation="MRQTLYDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16401..18418)
                     /gene="hyfB"
                     /locus_tag="RHX88_001889"
                     /pseudo
     CDS             complement(16401..18418)
                     /gene="hyfB"
                     /locus_tag="RHX88_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(18418..19035)
                     /gene="hyfA"
                     /locus_tag="RHX88_001890"
     CDS             complement(18418..19035)
                     /gene="hyfA"
                     /locus_tag="RHX88_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDV2230582.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNENLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(19288..19758)
                     /gene="bcp"
                     /locus_tag="RHX88_001891"
     CDS             complement(19288..19758)
                     /gene="bcp"
                     /locus_tag="RHX88_001891"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDV2230583.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(19758..20330)
                     /locus_tag="RHX88_001892"
     CDS             complement(19758..20330)
                     /locus_tag="RHX88_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDV2230584.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            20476..21354
                     /gene="dapA"
                     /locus_tag="RHX88_001893"
     CDS             20476..21354
                     /gene="dapA"
                     /locus_tag="RHX88_001893"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDV2230585.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21371..22405
                     /gene="bamC"
                     /locus_tag="RHX88_001894"
     CDS             21371..22405
                     /gene="bamC"
                     /locus_tag="RHX88_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDV2230586.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            22618..23331
                     /gene="purC"
                     /locus_tag="RHX88_001895"
     CDS             22618..23331
                     /gene="purC"
                     /locus_tag="RHX88_001895"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDV2230587.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            23486..23713
                     /gene="vapB"
                     /locus_tag="RHX88_001896"
     CDS             23486..23713
                     /gene="vapB"
                     /locus_tag="RHX88_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDV2230588.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSEDFMDIRDQPAMQERESF"
     gene            23713..24111
                     /gene="vapC"
                     /locus_tag="RHX88_001897"
     CDS             23713..24111
                     /gene="vapC"
                     /locus_tag="RHX88_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDV2230589.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            24258..25121
                     /locus_tag="RHX88_001898"
     CDS             24258..25121
                     /locus_tag="RHX88_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDV2230590.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            25136..27151
                     /gene="tmcA"
                     /locus_tag="RHX88_001899"
     CDS             25136..27151
                     /gene="tmcA"
                     /locus_tag="RHX88_001899"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDV2230591.1"
                     /translation="MAELTAFHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDTDSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHTATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAELEHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            27225..27923
                     /gene="ypfH"
                     /locus_tag="RHX88_001900"
     CDS             27225..27923
                     /gene="ypfH"
                     /locus_tag="RHX88_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV2230592.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(28033..28233)
                     /locus_tag="RHX88_001901"
     CDS             complement(28033..28233)
                     /locus_tag="RHX88_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDV2230593.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(28261..29388)
                     /gene="dapE"
                     /locus_tag="RHX88_001902"
     CDS             complement(28261..29388)
                     /gene="dapE"
                     /locus_tag="RHX88_001902"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDV2230594.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(29392..29748)
                     /locus_tag="RHX88_001903"
     CDS             complement(29392..29748)
                     /locus_tag="RHX88_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDV2230595.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         7235 a         7764 c         8007 g         6841 t
ORIGIN      
        1 gcctataaca cataatacag aggataatac tatgacgcgc cgtgctatcg gggtgagtga
       61 aagaccgcca cttttacaga caatcccgct tagtttgcaa catttgttcg ccatgtttgg
      121 tgcaaccgtc ctggtgcccg tcttatttca tattaacccg gcgactgtac tgttatttaa
      181 cggtattgga acgctgctgt atctcttcat ctgtaaaggg aaaattccgg cttatcttgg
      241 ttccagcttt gcctttattt caccggtatt gttactgttg ccgttagggt atgaagtcgc
      301 gctgggcggc tttattatgt gcggcgtgct gttctgcctg gtttctttta tcgtgaagaa
      361 agcggggacc ggctggctgg acgtgctgtt tccacctgcg gcaatgggcg caatcgttgc
      421 cgtcatcggt ctggagctgg cgggcgtagc tgccggtatg gcgggtttac tcccggctga
      481 agggcaaacg ccagactcca aaaccatcat catctctatt accaccctgg cggtcacggt
      541 tttaggttcc gtgctgtttc gtggtttcct ggcaattatc ccgattttaa ttggcgtgct
      601 ggtggggtac gcgctctctt tcgcaatggg aattgtcgat accacgccga ttattaatgc
      661 tcactggttt gcgctgccaa ccctctatac gccgcgcttc gagtggtttg ccattctgac
      721 tattctgcca gcggcgttag tggttattgc cgaacacgta gggcacctgg tagtaacggc
      781 taatatcgtc aaaaaagatc tgctgcgcga tccaggtctg caccgttcga tgtttgctaa
      841 tggcttgtcg accgtgattt ccggcttctt tggctctacg ccaaatacca cttacggaga
      901 aaacattggc gtgatggcga tcacccgtgt ttacagtacc tgggttatcg gcggggcggc
      961 gattttcgct atcctgcttt cctgcgtcgg taaactggct gccgctatcc agatgatccc
     1021 attgccggtg atgggcggcg tttcgctgct gctttatggt gtcatcggtg cttccggtat
     1081 tcgtgttttg atcgaatcga aagtggatta caacaaagca cagaacctga tcctgacttc
     1141 cgtgattttg atcatcggcg tcagtggcgc gaaggtaaac atcggcgcgg cggagttaaa
     1201 aggtatggcg ctggcgacca tcgtcggtat cggcttaagt ttgattttca aactgatcag
     1261 tgtgctgcgc cctgaagaag tggtgctgga cgcagaagac gcggatataa cagacaaata
     1321 atgtaccgac cgggcagtgt tgctgcccgg ttcaaacatc atgggattct gtggtaaact
     1381 tctcgcgatt ttgtgaaatc ctggttgagg tatctctgaa cacaccggca cagctctctt
     1441 tgccacttta tcttcctgac gacgaaacct ttgcaagttt ctggccgggg gataactcct
     1501 ctttactggc cgcgctgcaa aacgtgctgc gtcaggaaca tagcggttac atctatctct
     1561 gggcacgcga aggcgcgggg cgcagccatc tgctgcacgc ggcttgcgcg gaattgtcgc
     1621 agcgtggcga tgcggtgggc tatgtcccgc tggataaacg cacctggttt gttccggaag
     1681 tgctcgacgg tatggagcat ttgtcgctgg tctgtatcga caacattgag tgtattgcag
     1741 gcgatgagtt gtgggagatg gcgattttcg atctctacaa tcgaattctg gaatcgggca
     1801 aaacacgatt gttgatcacc ggcgatcgtc caccgcggca gttgaatctg ggattaccgg
     1861 atctcgcgtc gcgactcgac tgggggcaga tctacaaatt gcagccactt tctgatgaag
     1921 ataagttgca ggcgctacag ttacgcgcgc gtttgcgtgg ttttgaactg ccggaagatg
     1981 tggggcgttt cttgctgaag cggctcgaca gagaaatgcg cacgctattt atgacgttgg
     2041 atcagttgga tcgtgcgtcg attaccgcgc aacgtaagct gaccattccg tttgtgaaag
     2101 aaattctgaa gttgtagata aggtgtttat tgtcggatgc ggcgcgaacg ccttatccga
     2161 actacgcgaa gttaatccat agtattcgta ggccggataa gacgtggcaa gcgtcgcatc
     2221 cggcaatgtg cttcaactag cgatggagca catcttaacc cacgatttcc agtacctgct
     2281 ccggtggacg accaatccgc gctttgccat tcgcgaccac aatcgggcgc tccattagct
     2341 tcggattatc caccattgcc tgaatcagcg cctcttcgct aagcgaactg tcagccaggt
     2401 tcagttcttt ataaagatcc tctttctggc gcatcaattc acgggcgcta ttcatcccca
     2461 gcattttcag caaatcgcgc agcgttgccg catcggcggg tgtctcaagg tagagtacca
     2521 cttctggctc cacgccgttt tctttcagca ggttcagcgt ttcccggctc tttgaacagc
     2581 gtgggttatg gtagattttt acctgtttgg tcattgttct tcctttaatg cgaattacat
     2641 cttggtataa ggcttaaagc gttcctgcag ctggcgcaac tggtcgatgc gcgcatcgta
     2701 acgcgcttgt tgcaggctgc ctaatttcac ctgcgaactg gcgctactca acagcgaaat
     2761 ggcctgatcg agtcgtccgg cgagcgcata accttctgct cgcgcagcca gctcctgatc
     2821 gcggttattt agcgcggctt ccgcctgtgc cagtaaatcc cagccgttgc tgtcatcttt
     2881 attattaaag gtgtagcgat tcagaatatt ggccgcttct tgtggttgac cgccttgtag
     2941 ataagcgttc gccaggttga gctgcaacac cggattggtg cgcaaatcac gggcattttt
     3001 caggcgattg attgcctcat tggctttgtt ttgcccaaga tcgatatcgg tagccagatc
     3061 gagataccat gcgttgccag gttctgccgc cagtaacggt tgcagcgttt tacgcgcctc
     3121 gtcgtattta ttggcttcca tcgcctgtaa agcacgacca tattgcgccg ctcgttgctg
     3181 acgaacgttt cctttcgccc attcatccag caaatcactg gtgagctggt tacgtccgga
     3241 attatacatc cccagtgtgc gcgctttcgc cagatagaaa tcttccgacg actgcaccac
     3301 catcggtcgc atctgattag cacggttgcg ggcatctgcc agacgacttt ccggcaacgg
     3361 gtgagtcaat aaaatttccg gcgggcgcga ggagtaacgc gcctgatcga gtaatttttc
     3421 gaggaaggtt ggcatcgcct gcggatcgaa tcccgagcgt tgcagcacct gaataccaat
     3481 gcgatccgct tcctgttcat tttgctgggt gaagctgatc atcccctggc gcgttcccgc
     3541 cagtgtaccg gtcagcgctg ccatccccgc ctgcggactg gccatcgcca gtaaaataga
     3601 acctaacgcg ccgacccagg tcagcggcgc gctgcgctgt tgatcttcca tcgctcgcgc
     3661 caggtgacgt tgggtgacgt gggagatttc gtgcgccatt actgaagcca gttggctttc
     3721 gttatcggaa taacggaaca gggcagagtg cagtaccacg ttgccgccaa agaaagcaaa
     3781 ggcgttaatt tcgtcgttgt tgatcagaaa aaaatgaaac ggtgtcttaa ccgaattggc
     3841 atgcgaaacc agacgcattc ctagtgaatt aatgtactgc gttaatagtg gatcattaat
     3901 taacggcgcg ctgccgcgta gctggcggac ataatagtcg cccatctgca tttcctgacc
     3961 aatggaaagc gtgcttcctg cggaggttcc catatccggc aaggtgtctg cactatcggc
     4021 aaacgccggg gctacctgac caatggtcat ggcagcaatg agggttgcaa ccaggttttt
     4081 tttcaactgc ctgaacataa cctctatcct gtatttctgg agatagtcat ttgaccgata
     4141 ctgtgaaata tcgttcccac cgcgccgcct ttgagtgtaa cggcgaagat tagtccactc
     4201 aaatgacttt tcagtttcct gaatccgtca taaaaagcga agtctttttt gtgacatttc
     4261 gatacaattg cggatcgcaa tcccgctctt gaggttcagg gaaggtcttt atgctcgaaa
     4321 tgttgatgca atggtatcgc cgccgtttta gcgacccgga agcgattgcc ttgctggtta
     4381 ttttagttgc cggatttggc attatctttt tctttagtgg cctgcttgct ccgctgttgg
     4441 tggctattgt gctggcctat ttgttggaat ggccaaccgt gcgcctgcaa tctattggct
     4501 gctcccgccg ctgggcgacg tcgattgtat tggtggtttt cgtcggtata ttgctattga
     4561 tggcgttcgt ggtactgcct atcgcctggc agcagggcat ctacttaatt cgcgatatgc
     4621 cggggatgct caataagctt tctgactttg ccgccacgtt gccgcgccgc tatccggcgt
     4681 tgatggatgc gggcattatt gatgcaatgg ccgaaaatat gcgcagtcgg atgctgacca
     4741 tgggcgattc ggtggtgaaa atttccctcg cctcgctggt cggtttgctg accattgccg
     4801 tctatctggt gctggtacca ttgatggtct tcttcctgct gaaagacaaa gagcagatgc
     4861 tgaacgccgt tcgccgggtg ctgccgcgta accgtggact ggcaggacag gtgtggaagg
     4921 agatgaatca acaaatcacc aactatatcc gcggcaaagt gctggagatg atcgtggtgg
     4981 ggattgccac ctggctggga ttcttactct tcgggctgaa ctattcgctg ctgctggcgg
     5041 tgctggtcgg cttctcggta cttattccgt acattggcgc atttgtggtg accattccgg
     5101 tggttggcgt ggcgctattc cagtttggtg caggcacgga attctggagc tgcttcacgg
     5161 tgtatctgat tattcaggcg ctggacggca acctgttagt accggtgttg ttctccgaag
     5221 cggttaacct gcatccgctg gtgattattt tatcggtggt gatcttcggt ggtttgtggg
     5281 gattctgggg cgtattcttc gccattccgt tggcgacgct gatcaaagcc gtgatccacg
     5341 cctggcccga tgggcaaatc gcgcaagaat aatcttcagc ccgacgaact gtttcgccgg
     5401 gctgattttt cagggttcct gacgtaaata aatagcccga taacaaatac tcaccagcac
     5461 tgcaccgccg ataatattac ccagcatcac cgggagcaga ttggcggtaa taaaatggct
     5521 gaccgtcagt gccggaaaat tgtctgcgct actgtgcgcc agttgccaga aggaggtagg
     5581 ggcgaaatgg cgaatggcaa tggcgaacgg aatcacaaac aaattggcga tacagtgctc
     5641 aaagccactg gcgacaaaca gggtgatggg caaaatcatg gcgacgattt tgtcgcataa
     5701 cgaacgcccg caataactca tccacagcgc caggcaaacc atcagattgc acataatgcc
     5761 gaggctgaca gattcagtaa atgtatgatg cattttgccc tcggcgcagt gtaaaaccgc
     5821 gactccccac atcgcgtttt cactcatcac cagcccggaa aaccagatta acaaactgaa
     5881 acaggcaata cctgccagat taccgcaggc caccagaagt gcgttaatca gccaagttcg
     5941 ccaaccaata acgccccgac tttttgccat caccgtcatt accgacgagg tgaacaggct
     6001 ggtgccgcaa actgccagca aaataaagcc gagtgtaaag caaaggccgc ccaccagatg
     6061 ggttaatgcc tgtgaagacg gggcatctgc gataacggaa aggtaaaaag taaatccgat
     6121 cgccataaac acgccagcgg acatcgccat taaaaagacc gacactttac tgcgggcaat
     6181 tttatgggcg gcaatccgtt cagcgattgc cgcttttctg gcgctcaact gcaagtcgaa
     6241 agagagtttg tttctcatgc atactcctgc aaaagcagat tacaacacct cgcgaaccga
     6301 gatccccaga cgctgcattc gtgacagcag cgtggtgcgc ttcatcccta atcgtgtcgc
     6361 cgcgccacgg ggcccggcaa caatgccatt ggtttctcgc aatacctgaa caatgcgctg
     6421 acgttcttct tcgtcattct ctggcgtcgt cgggtgcagc aactgcgcca ttgaactgcg
     6481 aagcgctgaa tcttcattta gcgtcggcag taaacggctt tgtcggacat taagatgtaa
     6541 attcagactg ttaccgcgag tcagcagtac cgctcgctca atcacgtttt ccagctcgcg
     6601 cacgttgccc ggccagtccc acgacatcaa ctggcgtagc gcctcggtcg ggatggcgtc
     6661 aattgaacga ttcatatggc gcgccatttt ttgcgtgaaa tgctttgcta aaagagggat
     6721 atcttccgga cggtcgcgta gcggcggcaa ttccagtggg aagacattca ggcgataaaa
     6781 gagatcgctg cgaaactggc gatcttcaac catttgccac aaatcacggt tggtggcggc
     6841 aatgactctg acattcaccg ggatcgttct actcccgccg agacgctcaa tctcccgttc
     6901 ctgcaatacg cgcagcagtt taggctgaag ttctaacggc agatcgccaa tttcatcgag
     6961 aaacaacgtg ccgccatcgg caatttcaaa acgaccacga tgggtattaa tcgcaccagt
     7021 aaacgcccct ttgtcatgac cgaataactc actttccaga agactggcgg ggatggcagc
     7081 gcagttgatt ttgaccagcg gcttgtcgcg tcgcgggcta agttgatgga tcgctctggc
     7141 gatcacctct ttgccggttc cggtttcacc gcaaatcaac accgtactgt cgctcttcgc
     7201 cacaatatct acctgctgga gtaggtcttc cattgcctgg ctttgataga taatgtcacc
     7261 gatgcccaga ttcgaaagga gctgctcgct aagctggtgg ttttcttgct gcaaactttc
     7321 ctgcaaatcg gtcatgctac gccaggcatc ggcattgcca acggcaatag cgatgcgatc
     7381 ggctatgtgt tgtagtagct ggcagttttc ctcactaaag agagtggaag aggtatgcgc
     7441 cagcaacaat gcacccggtg tatggttgcc aaaggtaagc ggtataagga gcgcagattc
     7501 gcagccgtta gatgcaagta agagtaataa cgggtcgcgt tgccagagaa acagatcgtc
     7561 tgcacggtgc gtcaaggtcg gttgtcgggt ttgtagcgtt tgtgtcagca atacactgtc
     7621 gccaggcata ttgcgtggca gacacgcaca atgtgaggca gaaagatcgc tgcaccacag
     7681 gctaaacttc tcgttctttc gatgatcgcc cagtaccata ctgactgaag ccagaccgaa
     7741 aaaatgatgg atctcacgag cgacgtcagc gatcagatcg tcgagatcaa gatgtgacag
     7801 cactgcatta gtaatatcaa ccagtacccg atgatgatcg cgatctttct ccagcaaacg
     7861 cagcgccacc tgctgttctt catcattgag ccatgattgc agaaccgggg tgattatccg
     7921 cgccagccag ttaaacagtg tgcaatcctg gggagtaaaa cggctgccgt gggtacgaat
     7981 aaagcgtagc tccgccattc cactactgcc gagaaggacg gcgtgacgcg aataatcgac
     8041 catctcgccc tgatactctg ttgttgccgt cacatcgctg ccatcatcat ggtagcggta
     8101 atagaagttg cttttgagaa catgcagttc aataagttgt accgggctga acggcggcgg
     8161 catcgggatg aaggcgcgta ataaagacgc cttaccgtgt ttctgtgcta accgctccgc
     8221 gagcattccg tttaccgctt caattgttat tccttgtggc ggggcaaaca tcgcctcgtc
     8281 tgacatagcc atcaacagtc cttattaatg agaaatcctt ctcacgcaat ttttctggcc
     8341 atcagataga tggccggttc ctgcacaatt tcatccaaca gatgaatgaa ttgaatgcta
     8401 cgcgcctgcc acacagacgt cgcgtcatcc gccagtggcg cgaatgcccg ggtcaacagg
     8461 gcggtgtggc tggcgtcaat aacaatctca ccaaaattca tcacccccag catcttacgt
     8521 cgggcttgct cctcttcgac ctgggcaagc caatcttcgt attccgtcag tgggcagggg
     8581 aaggcgacat tcagacaatc aatcacgcca acgtgatgac cgatagccag cgagtaatac
     8641 attacctgag agctgtgttc cggcatctcg tcgctactgg cgacaaactt ttttcgcagc
     8701 gtccagaaaa caatttcccc gcactcttca gtcatgtaac ccccggagta cgcgaaagag
     8761 ttgctgaacg atactattga gacgtggatc gtcggcatcg cgcaaccagg tgttaacccg
     8821 atttcctgta ggatcgtcgc ttaaatggcg caggagccga tcgcaaattt ctcgcccctg
     8881 acgatagccc gccagccgcc gcgcttctcg ctcaatggcg atacgctgtg atggcgggat
     8941 ctgcggccac aacggttgca tagtcacccc ggtgggatcg cgataatcca ccgcgtgaat
     9001 cttctgttgc agcaaaccga gcgccacggc gaaaccgtga atggtggcgg ccggtgttgg
     9061 cgggcagccg gggatccaaa catcaatggg gacaatggtg tcgctgccgc cccagacgct
     9121 gtagagatcg tggaaaatcc gccgccggca ccgcacgcgc cgtaggaaac acagatttta
     9181 tgatcggggg cagactcata cgcccgaagt gcaggcatac gcatcgcccg ggtgactgcg
     9241 cctgtaaata acaaaatatc ggcgtgacgc ggtgatgaaa taaccttaat gccaaaacgt
     9301 tctgcgtcga atactggtgt gatggcggca aaaatttcga tttcacaggc gttgcagccg
     9361 ccgcaatcga cgcgataaac gtaagccgag cgacggatat cctgtagcaa atgctgcttc
     9421 atcttttgcg tttgctcgtc cagcgtgatg ggctggctga catattgtgt aagcactgga
     9481 ctcatagctg ctccttagcc accagcggta catctgtatc gtcgttgagc agcgtcgcgc
     9541 gttgtttgca ctccgggcag acgctcgctt gcgcccataa catttcgcgg ttttgtggcg
     9601 cattttgttg ctctgccaac aattcagcag ccagtgcgac ggttttttgc tgggcaaacg
     9661 ggcgttcgca acggctgcaa cgttgtagat ggaacgtcgc gcgggtatag agatcggctt
     9721 tattggtgac ggtcggttca aagttattgg taagctggat ggctctggtc gggcacactt
     9781 cttcacaacg tccgcagtaa atacaacgcc tcagatagag ctgccaggtg cgcgaatttt
     9841 gctggtcgtc ggtctggata gtcagcgcat ttgccggaca agcacaggcg caggcaccgc
     9901 aggcaataca ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca
     9961 gtggcgcgaa gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact
    10021 tcagcatctt ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga
    10081 tctctttata cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt
    10141 cggtacagga gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat
    10201 tgccgcgcag catgtaacgc aacaccggcc agttggcgta ggtggcggca cgacaacgcc
    10261 agcggaacaa tttttggtta tcaccgagca tgctccagtg cacgtcttcg ccgcgtggcg
    10321 cttcaacaaa gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg
    10381 gggtatccgg catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt
    10441 tgacacggac catcatgcgg gagaagacat cgccgccggt aaaggtaaac agtgtttttg
    10501 gaatattgcc gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc
    10561 cgcggatcag cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa
    10621 tgccctgagt gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca
    10681 cgtcggcgcg catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat
    10741 cgcggcgaac accaccaatc agattcagac cgtaggtttt acgcgacccg atcagcaatt
    10801 ccgccatcgt catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaaac
    10861 caacgaaatg gcaggagagg ccaaggttaa gcaaatgact gtgtagccgt tcgacttcca
    10921 gcagaatcga gcgaatagta tgtgctcgtt gcggcacctc aatccccagt gcattttcaa
    10981 ccgaattggt ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cggtccgata
    11041 agaaggtcac ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga
    11101 cataaaacag gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt
    11161 gacccggttc atcggaggtg atatgcagcg ggccgacagg gatcacccgc gcatcgctgt
    11221 tgccctcatt gataaacgca tacgtttcgg aatctgtcgt cggttcaggg cgcagtcgat
    11281 aatccatcgc atctttgcgc agcggatgca tatcttccgg ccagtcatcg ggcaacacca
    11341 ggcgacgctg atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct
    11401 cgccccagac cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat
    11461 cgaccagtgc cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat
    11521 aaaccgcgta atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc
    11581 catcatgttg ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa
    11641 tggtgacctg ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt
    11701 tcagggcggc gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc
    11761 tccgaaacag atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc
    11821 aggtcgtgcg tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt
    11881 tgtggaatat gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg
    11941 gtcaaccagc cgagattacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc
    12001 gctgccatcc gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata
    12061 atcagcaggt gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg
    12121 ttgaagggcg gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat
    12181 ggcatgattt tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt
    12241 acattgccgg aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc
    12301 agcgcggcaa aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg
    12361 ttctccacgc tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat
    12421 gccgccacgg caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg
    12481 ccgatggctt ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc
    12541 aatacggcgg agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc
    12601 caggcgtgca tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag
    12661 gccaacagta ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat
    12721 atcgccattt ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg
    12781 aacagaccaa aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc
    12841 gatgaacgct gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc
    12901 gccgcccaca tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac
    12961 agatggaaga aaccgtagta atcgcacagt gtaacgggtg aaagctcgcc gtgtgccact
    13021 tcatgacgca tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg
    13081 gcgaggaaca aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct
    13141 cctgcatcag cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct
    13201 aagctatgta atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa
    13261 cagagcagcg aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc
    13321 atctcattat cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca
    13381 tatttttctt gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc
    13441 tatttccacc agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt
    13501 ttccatcagg cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag
    13561 caggccaagc agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat
    13621 cggtagcttc acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag
    13681 tgccatcatt gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc
    13741 agcgtaagtc atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca
    13801 gatcagcagt tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac
    13861 cagcgactgg caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa
    13921 cagcgatgtc agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca
    13981 tcatctcctt actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc
    14041 agtacaatca acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg
    14101 ctcggtttgc caggcacaac gcgaccaaac cagcgaataa accaggcgaa actggcgacc
    14161 gattcaatca tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca
    14221 aaaccggcag caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacgccggta
    14281 atcgccagcg ctgccacgca gaaacccacg cctggcaacg gcagggtgtg caatacgcca
    14341 cgcagacgcg gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa
    14401 aacaggcttt tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc
    14461 cgcgagccga agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc
    14521 gaccaggcca gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga
    14581 atggtgacca gtgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg
    14641 tcgataattg cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga
    14701 taggcgctga tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc
    14761 tgggccgatt tcccccacgc ggcaaacagg atcccgccat aaaccagata acgtgcgtcg
    14821 ccgtgtaact cgctcattgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg
    14881 gcggcaagat acaaccccaa cgaaccgata tgggtgataa gcaacgcttt tagtgctgaa
    14941 cgctgtgctt tatcgctctg gtaataactg atcaacgccc aggagcagcc gccctaattt
    15001 caaaaaacaa caactgaccg agcagcgtcg aggagagtac cagtcccgcc atcgcgccga
    15061 taaacaccag caaaaatgcg taataacgat tcgtgccgtt atgcgggtgt tcgcgatttt
    15121 tatccgtcag ataacccgtg gagtagatcg tgaccagcaa accgagaaac accaccacaa
    15181 acagaatcag cgtactcacg cgatcaatga ccaggccaaa caatgccaca tcccccacgt
    15241 tgaccaacgt caacgtgacg gcaactttat cggcctgata aaacgcggag atcagcgaca
    15301 acatgcacag cgtggtcagt gcggcgaaca aaacccccat tcggcggccc gacgttgtgg
    15361 cgaaaacgaa acgaccagtg cgccgataaa aggcagcatt aacgtcgtca gagcaagatt
    15421 ttccatattc cgtcagtgct ccttacagac cggttaacca gaagacccat gcaagcgcag
    15481 caaggctgaa gccaagccag gtcacatggt gaatgagtaa aaaacgcccg cgtgccagcg
    15541 tgttttctgc gattgaggcc agtacaaaaa tcagcaaaac cttgagcagc gtaacgacaa
    15601 gtgaagtcag caggcaggcg agagaaagtt cttgcgcgcg cccaaagggc agaaacaggg
    15661 ccacaaacag tgctgccatc acgacctgtt tcagccccag cccccatttc gctagcgcca
    15721 gcccggcacc ggaatattcg gtcagcgggc cttcctgtaa ttcctgttct gcttcagcaa
    15781 catcaaaggg aatttttccc atctcaatga agcaggcaaa accacaggcc agtaacgcca
    15841 gtacggtggt tagcggcgag ttccagcccg ttgccagcgt attgctgatc atctcgatat
    15901 gcgtggaacc tgctatcagc gccaatacca gcagtgagag aatgagcatg ggttcgacca
    15961 gaatgccgag cgtcaactcg cgactggcac cgactcccgc aaacgggctt ccggtatcca
    16021 gcccggaaag agcaaagaaa aaacgaaaca gggcaagaag atagataagg gtgatcagat
    16081 cgccgccgcc cgcaaaaggg gaaacggtaa taaaaagtgg tagggccatc gccagcacca
    16141 gcatgctgct gattaatacc cacggcatca ggcggaacat cagacctgaa gatataggcg
    16201 caacttcctg gcgtttaaac agtttgtgga tatcgcgata atcctgccag atccccggcc
    16261 cgcggcggga gtgcatacgc gcgcgtatct gccgggaaat acccgtaaaa agcggggtta
    16321 gcatcagcag aatcacggcc tgtgctaacg caaaaatgac cagatatccg tcgtaaagag
    16381 tttgtctcat ggtgatttcc ttagacggca atagcgatta gcagcacaac cagtgcggcg
    16441 accacataca ggcaatagag acgaaagtcg ccgctttgca gatgctgtat ttctttggcc
    16501 agccgttggg tggcgctcac gatggggcgg atcacccgct catcccagaa gggttctgtg
    16561 ctctgagccc tggcggtgac gtgcgcaaga cctttattta gcctcagcgt agggtcgaac
    16621 tgttgtcgta gacgaaatag cgcagaaaag accacacgca gcggctgcat cacgccattg
    16681 cctgacgggg ccatcgcatt ttcccagccg tagccgcatg cccaggcatc tcctgtgcga
    16741 cgaaacgctg cgcggcgcga acgacaccac agccagtaaa ggccaggcag caaaggcatc
    16801 gccagtagta acagcaacag taatgagggg gtgacccgtg tatgaaacgt gccgggtaca
    16861 agtgctattc cgctggcgac agtgacggga ggggtattgg taaacgcatg ggcaatatgc
    16921 attatcttcg gtgccagcca actggcacta agcgcaatta atacgcagag tgccgcgagc
    16981 agtagcatgg cgacgatcat cgtatttggc acttcctgtg cctcttcagc gtgtgtactg
    17041 cgcggcgcac cacagaaagt aataccgtac attttgacga agcacattac tgccagccca
    17101 ccagtgactg ccagcattac catagcaata ggacccgcaa gttgtagcgc tacggcttcc
    17161 acacgactta gtgagaacag cgactgccag gtgtaccatt cgctgataaa accattcagc
    17221 ggaggaatgg ctgatatcgc gaggcaacca atcaggcatg ctgcggctgt ccacggcatc
    17281 cgtttcgcca gtgcccccat tttttccatg tcgtgggtat gcaaacgcga aataatcgca
    17341 cccgcgccga gaaatagcag ccctttgaac agcgcatggt tgagcagatg aaacagtgcg
    17401 ccgagcagtc caaccacggt gagcagcggg tcgtgcagtg acagaccgtc catcgccaca
    17461 ccgaccgcca gcaaaataat gccgacgttt tcgacggtac tccaggccag cagccgtttg
    17521 atatcctgtt ccgccagtgc atacagcacg cccaggagcg cggagattgc gccgatcgcc
    17581 atcaccagag tgccccacca cagaggcaaa cccgtttgcg ccagcagatc catcgctact
    17641 ttcaggatgc cgaaaatacc gattttgacc attacgccag acatcaacgc cgaagcgtgc
    17701 gatggtgcgg cagggtgagc gcgcggcaac cagctgtgca acggcatcat cccggctttc
    17761 gcgccaaaac cgaaaaaggc cagcagaaac accgccgacg ccagccccgg agaaagtgaa
    17821 agcgtgcgaa actggcaaaa tcgaggctgc cgctttcgcg ccccatcagc aagaaggcga
    17881 tcataatcag caccgatccg gcgtgggcga taaaaaagta gagcatgcca gcgttgatcg
    17941 acgttttatc ctgcctggca atgaccagaa accaggaaga cagcgacatc atttcaaaca
    18001 gcacgatgaa ccaaaaagcg ttgtccatca ccagcagggc aaccatcgat gcgatgaaaa
    18061 gattcataaa gaagcccatc gccgccgcgc ctttgccctc gtattcgcgc atataagtca
    18121 atgaatagag cgaacaaacc accaccagca aggagataac cagcaccata aatgccgaga
    18181 ggctatccat tcgcagggtt aaatcggcaa agtcaaaagg ggtggcaaaa tgggcgacta
    18241 aagttacacc agcatgtaat tgcgtaattc cgctgatgat gccaatcacg ccgcccacca
    18301 gcgatgtgat gccggaaagc ttaatagcca gtctgtccag accgagtaaa aacagcgaag
    18361 ccagactggc aaacagatag aggatcagcg accaggttaa taattgcagg gcatccatta
    18421 gtgttgctcc tgagtgaggg aaaggggatc ctccccgcca ttggcaaacc agcttgcggc
    18481 aaggcgctgt ttttctttca tctgacgttg caggctatcg tcggtgatca gccgtaacgc
    18541 ctgattcggg caagcgcgaa cgcaggccgg accttctggc aagaaatcac acaggtcgca
    18601 tttcaccgcg acggtctgga cacccggttc ccagcgcagc aacgcatgtt ggggcagcac
    18661 gttttcttcg ccgtctttga gtgagccttc agcctgaaaa acatattgcg catgggcatt
    18721 caccggacgg cttcctgaag cgctgattgc gccaaatggg cagaccacgg cgcaaagctt
    18781 gcagccaata cagaggtttt cgttgagttg gatcgcatca tccctctgag agatggcatt
    18841 gaccgggcag acctgcaggc aaggggcttc ctcacagtga tgacacacga caggggcagt
    18901 gattgttgac gtcttcgcca gggccaggcg cgggtgttgc tgtaaacctt gcgttttatg
    18961 cacgtccgaa caggcagcga gacaggtatt acatcctgta caccacagtg gttcggccac
    19021 cacaaagcgg ttcatgtcgg ctccttgaaa atgatatgaa acattgcatt tcatattgca
    19081 taaaggatgc cattctgatt aactcaatat attcaataag ttatgtatta actggattct
    19141 gaacggtaca aaaatgatgg tcattaacgt tcagcaaaaa atggattcga cacccgtgtc
    19201 gcaggggaaa gcgagaatgt gatcttgatc acgagaagtg aggtatgctg gccgcaagcg
    19261 cagccagcac ggaatggagc aaagtaatca ggcgtgttct ttcagccagt tcagcacaac
    19321 gtcgtggtga ttgctggttt tgaaatcgtc aaagacatgt tcgattttgc catcagcgtc
    19381 aatcaggaag ctgatgcgat gaataccatc gtaggttttg cccatgaagg acttttcacc
    19441 ccagacgccg aattgttcgc acacctggtg gtcctcatca gacaggagcg taaagttaag
    19501 cagctctttt tccgcaaaac gggagagttt ttcgggttta tcggtgctga tacccagcac
    19561 atcaacgccc gcttttttca actcatccat gttatcgcgt aagccgcagg cctgtacggt
    19621 acagccgggg gtcatggctt tcgggtagaa ataaaccaga acacgctgtc cctggaagtc
    19681 ggtcaaatta acttgttctc cgtcttgatc cggcaagcta aatttcggtg cgatatcacc
    19741 ggctttcagt ggattcatta cttaactcca tcctgttcat catgttggga ataattgacg
    19801 acgttaatac tgccttgtgc attgagttct gtacataggg ctttgaacgc ttgctcaata
    19861 tttgctgcgt ccgcagatgc ggggctgtgg gcggttatct gaatatgcaa ctgcgcagcc
    19921 cgttcatttt cagcaggttg cgtgcgcgac accagctccg caatgttcat atgatgcgcg
    19981 tcgaaaagtg ctgtgaagcg ttcaattaaa tgcggggagt ctgccacatc gacctgaacc
    20041 cagacagatg ctggcattgg cggacgcgga cgcgccgtcg tgcgcttcat cacgattaaa
    20101 agatccagtt cggcaccttt caacggtaac gttgattcaa tcagagtaat ggcattccat
    20161 gaaccggaaa gcagcataat aaacgtgaac tcttctccca gcatcgccag gcgactgtct
    20221 tcaatattac agccgcaact actgacatga cgggtgatgg tgttcacaat tccagggcga
    20281 tcggcaccca acgcagtgat caccagataa tgttgcgatg acagtgtcaa actggttatt
    20341 cctttaaggg gtgagttgtt cttaaggaaa gcataaaaaa aacatgcata caacaatcag
    20401 aacggttctg tctgcttgct tttaatgcca taccaaacgt accattgaga cacttgtttg
    20461 cacagaggat ggcccatgtt cacgggaagt attgtcgcga ttgttactcc gatggatgaa
    20521 aaaggtaatg tctgtcgggc tagcttgaaa aaactgattg attatcatgt cgccagcggt
    20581 acttcggcga tcgtttctgt tggcaccact ggcgagtccg ctaccttaaa tcatgacgaa
    20641 catgctgatg tggtgatgat gacgctggag ctggctgatg ggcgcattcc ggtaattgcc
    20701 gggaccggcg ctaacgctac tgcggaagcc attagcctga cgcagcgctt caatgacagt
    20761 ggtatcgtcg gctgcctgac ggtaacccct tactacaatc gtccgtcgca agaaggtttg
    20821 tatcagcatt tcaaagccat cgctgagcat actgacctgc cgcaaattct gtataatgtg
    20881 ccgtcccgta ctggctgcga tctgctcccg gaaacggtgg gccgtctggc gaaagtaaaa
    20941 aatattatcg gaatcaaaga ggcaacaggg aacttaacgc gtgtaaacca gatcaaagag
    21001 ctggtttcag atgattttgt tctgctgagc ggcgatgatg cgagcgcgct ggacttcatg
    21061 caattgggcg gtcatggggt tatttccgtt acggctaacg tcgcagcgcg tgatatggcc
    21121 cagatgtgca aactggcagc agaagggcat tttgccgagg cacgcgttat taatcagcgt
    21181 ctgatgccat tacacaacaa actatttgtc gaacccaatc caatcccggt gaaatgggca
    21241 tgtaaggaac tgggtcttgt ggcgaccgat acgctgcgcc tgccaatgac accaatcacc
    21301 gacagtggtc gtgagacggt cagagcggcg cttaagcatg ccggtttgct gtaaagttta
    21361 gggagatttg atggcttact ctgttcaaaa gtcgcgcctg gcaaaggttg cgggtgtttc
    21421 gcttgtttta ttactcgctg cctgtagttc tgactcacgc tataagcgtc aggtcagtgg
    21481 tgatgaagcc tacctggaag cggcaccgct tgcggagctt catgccccgg ctggaatgat
    21541 tttgccggtg acctccggtg attatgcaat cccggtgacc aacggtagtg gtgctgtcgg
    21601 taaggcgctg gacattcgtc caccagccca gccgctggca ctggtttctg gcgcgcgtac
    21661 ccagttcacg ggcgataccg cttcattgct ggtggaaaat ggtcgtggca atactctgtg
    21721 gccgcaggtg gttagcgtgc tgcaggcgaa aaactacacc atcacccaac gtgatgatgc
    21781 tggtcagaca ctgaccaccg attgggtaca atggaaccgt ctggacgaag acgagcagta
    21841 tcgtggtcgt tatcaaatct ctgttaagcc gcagggttat cagcaggcgg ttacggttaa
    21901 actgctgaac ctggaacagg cgggcaaacc ggttgcagac gcggcttcca tgcagcgtta
    21961 cagcacggag atgatgaacg ttatttccgc cggtctggat aaatctgcca ctgacgccgc
    22021 gaacgctgcg caaaatcgtg cctccaccac tatggacgta caaagtgcag ctgatgacac
    22081 cggtttacca atgctggtcg tacgcgggcc gttcaatgtg gtttggcaac gtctgccagc
    22141 ggcgctggaa aaagtgggca tgaaagtgac cgacagcacc cgttcgcagg gcaacatggc
    22201 cgtaacttat aagccgctgt ctgacagcga ctggcaggaa ctgggcgcga gcgatccagg
    22261 cctggcatcc ggtgactata aactgcaggt tggcgattta gataaccgca gcagcctaca
    22321 gttcatcgat ccgaaaggtc atactctgac tcagagtcag aacgacgcgc tggtagctgt
    22381 cttccaggct gcgtttagca agtaaaaata cagggctgga atcatccggc ccttttttct
    22441 gatatgatac gcaaacgtgt gcgtcggcag gaaaacgcga ttttagcggt aattcgcacg
    22501 aaatttgttt gtcggacgta gttcggataa ggcgttcacg ccgcatccga caaaacatcc
    22561 ggcacaccag acagcaaaag attttaaaac gttaattcac acccaggagt gataaagatg
    22621 caaaagcaag ctgagttgta tcgtggtaaa gcgaaaaccg tatacagcac ggaaaacccg
    22681 gacctgttgg tgctcgaatt ccgcaatgat acgtcagcag gggatggcgc gcgcattgag
    22741 cagtttgatc gcaaaggtat ggtgaacaac aagttcaact acttcattat gagcaaactg
    22801 gctgaagcgg gtatcccgac tcaaatggag cgtctgctct ccgataccga atgtctggtg
    22861 aaaaagctgg atatggtgcc ggttgagtgt gtcgtgcgta accgtgctgc tggctctctg
    22921 gtgaaacgtc ttggaatcga agaaggtatt gagctgaacc cgccgctgtt cgatctgttc
    22981 ctgaaaaacg acgccatgca cgatccgatg gtcaacgaat cttactgcga aacctttggc
    23041 tgggtgagca aagagaacct ggcgcgtatg aaagagctga cctacaaagc gaacgacgtg
    23101 ctgaaaaaac tgttcgatga tgctggtctg attctggtcg acttcaagct ggagtttggt
    23161 ctgtacaaag gtgaagtggt actgggcgat gagttctccc cggacggcag ccgcctgtgg
    23221 gacaaagaaa cactggagaa aatggacaaa gaccgtttcc gccagagcct cggtggcctg
    23281 atcgaggcct atgaagccgt cgcccgccgc ctgggtgtac agctggactg atttttctgg
    23341 ttgcttgttt atttagccgg agggtactcc ggctaaatgt cttcgatacc cccgctacaa
    23401 tacttcgcat tatctccctt tgacaggaaa gtaataacag gccaatatgt atatacatga
    23461 cgtatatccc aaaaaagggg gggctatgga aactactgta tttttaagta atcgcagtca
    23521 ggccgtcaga ttaccgaaag cggtcgctct gccagaggat gttaagaaag tagaaattat
    23581 tgcgattggt cgtacaagaa tcatcactcc cgccggtgag tcctgggaca gttggtttga
    23641 tggcgaaaat gtgagtgagg attttatgga tatccgtgat caacccgcaa tgcaggaaag
    23701 ggaatcattc tgatgctgaa attcatgctg gataccaata tctgtatttt cactattaag
    23761 aataaaccag tacatgtcag agagcggttt aacctgaatt ctggccggat gtgtataagt
    23821 tctgtaacat tgatggagct tatctatggg gcagaaaaaa gtcagatgcc agaacgcaat
    23881 ttggcggtga ttgaaggttt cgtatctcgt ttggaagtgc ttgattatga tagtgctgct
    23941 gcgacgcata ccgggcaaat cagggccgag cttgcccggc agggaaggcc tgttggacct
    24001 tttgaccaga tgatagcggg acatgctcgc agtcgcggtt tgattgtggt taccaacaat
    24061 acccgagagt ttgagcgagt ggcgggaatc agactagaag attggagcta acattctctt
    24121 ttacttctaa cattcgtaaa ttatataacg tggcatgaag ccttaattcc tccgccattt
    24181 catcccgctt tcctgtggta atcttcctct gaaccaccta cgatcatttc tataatgaat
    24241 atattgttga ggtatctatg cgttggcaag gacgacgtga aagtgacaat gttgaagaca
    24301 ggcgcaacag ctctggtggt ccgtctatgg gcggtcccgg ttttcgcctg ccaagcggta
    24361 aaggcgggct gattttactg atagtcgtgc tggttgcagg ctactatggc gttgatttaa
    24421 ccgggttgat gaccgggcag ccggtttccc aacagcaatc aacgcggtca attagcccta
    24481 atgaagacga agccgcaaaa ttcacctcgg tgattctggc aaccacggaa gacacctggg
    24541 gacaacagtt cgagaagatg ggtaagacct atcagcaacc gaagctggtc atgtaccgtg
    24601 gaatgacgcg taccggatgc ggggcgggcc agtccataat ggggccgttc tattgcccgg
    24661 cggatggcac ggtttatatc gatctctcct tctatgatga catgaaagac aaacttggcg
    24721 cggatggcga ttttgcccag gggtacgtta tcgcccatga agtcggtcat catgtgcaga
    24781 aactgttagg catcgagccg aaagttcgtc aactgcaaca aaacgcgacg caggcggaag
    24841 tgaaccgctt atctgtgcgt atggaactcc aggccgactg ttttgccggt gtctgggggc
    24901 atagtatgca gcagcaaggc gttctggaaa ccggcgatct ggaagaggcg ctgaacgcgg
    24961 cgcaggccat cggcgatgac cgtttacaac agcaaagtca ggggcgagta gtaccagaca
    25021 gtttcactca tggcacttct cagcaacgct acagctggtt taaacgtggt ttcgacagcg
    25081 gcgatccggc acaatgcaat acttttggta aaagcattta acttccgggg cagggatggc
    25141 tgaactgact gcgtttcaca cattaacagc gcaaatgaaa cgtgaaggga tccgccgctt
    25201 gctggtgttg agcggggaag agggttggtg ttttgatcat gcgcttaagt tgcgtgatgc
    25261 cttacctggc gactggctgt ggatttcgcc gcagccagat gctgaaaacc actgttctcc
    25321 ctcggcacta caaactttac ttgggcgcga gttccggcat gcggtattcg acgcccgcca
    25381 cggctttgat gccgctgcct ttgcggcact tagcggaacg ttgaaagcgg gaagctggct
    25441 ggttttgtta ctccctgtat gggaagagtg ggaaaaccaa cctgataccg actcgctgcg
    25501 ctggagtgat tgccctgacc ctattgcgac gccgcatttt gtccagcatt tcaaacgtgt
    25561 acttacggcg gataacgacg ctattctctg gcggcaaaac cagccgttct cgttggcgca
    25621 ttttactccc cgtactgact ggcacaccgc gaccggcgca ccacaaccag aacaacagca
    25681 actcttacag cagctactga ccatgccgtc gggcgtggca gcggtaacgg ctgcgcgtgg
    25741 gcgcggtaaa tcggcgctgg cagggcaact catttctcgt attgcgggta gtgcgattgt
    25801 cactgcgccc gcaaaagcgg caacggatgt actggcacaa tttgcgggcg agaagtttcg
    25861 ctttattgcg ccggatgcct tgttagccag cgatgagcaa gccgactggc tggtggtcga
    25921 tgaagccgca gccatacctg cgccgttgtt gcatcaactg gtatcgcgtt ttcctcgaac
    25981 gttgttaacc actacggtgc agggctacga aggcaccgga cgtggttttt tgctgaaatt
    26041 ttgcgctcgc tttccgcatt tacaccgttt tgaactgcaa cagccgatcc gctgggcaca
    26101 gggatgcccg ctggaaaaaa tggtcagcga ggcactggtt tttgacgatg aaaacttcac
    26161 ccatacacca caaggcaata tcgtcatttc cgcatttgaa caaacgttat ggcgaagcga
    26221 tccagaaacg ccgttaaagg tttatcagtt attgtctggt gcgcactacc ggacctcgcc
    26281 gctggattta cgccggatga tggatgcacc agggcaacat tttttacagg cggctggcga
    26341 aaacgagatt gccggggcgt tgtggctggt ggatgagggc ggattatctc aacaactcag
    26401 tcaggcggta tgggcaggtt ttcgtcgccc gcggggtaat ctggtggccc agtcgctggc
    26461 ggcgcacggt agcaatccac tggcagcgac attgcgtgga cggcgggtca gccggatagc
    26521 agttcatccg gcgcgtcagc gggaaggcac agggcagcaa cttattgctg gtgctttgca
    26581 atatacgcgt gacctcgact atctttcggt gagttttggt tacaccgggg agttatggcg
    26641 tttctggcac cgctgcggtt ttgtgctggt gcggatgggt aatcatcgtg aagccagcag
    26701 cggttgctat acggcgatgg cgctgttacc gatgagtaat gcgggtaaac aactggctga
    26761 acttgagcat taccgtttac gtcgcgatgc gcaagctctc gcaaagtgga atggcgaaac
    26821 gcttcctgtt gatccactaa acgatgccgt cctttctgac gacgactggc ttgaactggc
    26881 cggttttgct ttcgctcatc gtccgctatt aacatcgtta ggttgcttat tgcgtctgct
    26941 acaaaccagt gaactggcat taccggcgct gcgtgggcgt ttacagaaaa acgtcagcga
    27001 cgcgcagtta tgtaccacac ttaaactttc aggccgcaag atgttactgg tccgtcagcg
    27061 ggaagaggcc gcgcaggcgc tgttcgcact taatgatgtt cgcactgagc gtctgcgcga
    27121 tcgcataacg caatggcaat tttttcactg actccttcaa ttttctccca tggtcattgt
    27181 gagtcagcgg cgtaaaatta atgacatcag ttaaggagtt cgccatgaaa catgaccatt
    27241 ttgttgttca aagcccggat aaacctgcgc aacagttgtt actgcttttc cacggtgtcg
    27301 gggataaccc ggtggcaatg ggggaaatag gtagctggtt tgcaccgctg tttcctgatg
    27361 cgctggtggt gagtgtcggt ggcgcggagc cgagcggtaa tccggcgggg cgtcagtggt
    27421 tttcggtgca gggtattacg gaagataatc gccaggcgcg tgtggatgcg atcatgccga
    27481 cgtttattga aacggtgcgc tactggcaga aacagagcgg agtaggggct aacgccacgg
    27541 cgctcatcgg tttctctcaa ggcgcgatta tggtgctgga gagcattaaa gccgaaccgg
    27601 gccttgcttc acgcgtcatt gcttttaacg gacgctatgc gagcctgccg gaaactgcgt
    27661 ctacagccac gacaattcac ctgattcatg gcggtgaaga tccggttatc gatctggcgc
    27721 atgcggttgc ggcgcaggaa gcgttaatca gtgccggtgg tgacgtgacg ctggatatcg
    27781 tggaggattt aggccatgcg atcgacaatc gcagtatgca atttgccctg gatcacttgc
    27841 gttataccat tccgaaacat tatttcgacg aagcgctaag tggcggtaag cctggcgatg
    27901 atgatgtgat tgaaatgatg taaaagacag attacctggc ctgataagac gcgtgagcgt
    27961 cgcatcaggc atcgttcacc attgccggat gcggcgttaa cgccttatcc ggcctacggt
    28021 tgagcactca gcttacttct tcggttgatc ctttttcggc cagtcgtcgt catcgtccca
    28081 cttatcgttg aaatcacgat gcgggggaag ctccggcttg ttggcgagaa atttcttatg
    28141 gtcgacgcgc ttgagatctt taatcacgtt cagcaggacg cctaccaaaa agacaatcac
    28201 cagaatccac caatatttag ccagccagtc catgcttatt tcctcttgca gaaccactca
    28261 tcaggcgacg agctgttcca tgatacgttg atacatacgg gcaagtagct gcaggtcggc
    28321 agcgttcaca cattcattaa ttttatgaat agtggcattg accggcccga gttccaccac
    28381 ctgcgccccc atgcgagcaa taaagcgccc gtcggacgtt ccgcctgtgg tcagtagctg
    28441 cggtttaatt tcattatagt gctcaaccgc gttaacgacc gcatccacca gtttaccgcg
    28501 cgcggtcaaa aatggctgcc cggaaagcca ccagtccacc gtataacgca gttgatgttt
    28561 ttcaagcagg gcaagcacct gtgctttgat catctcatca gtcagttcgg tgctgaagcg
    28621 gaagttaaac tgcacaaaca gttcacccgg aataacattg ttactgcccg ttcccgcctg
    28681 aatattagca atctgcatac tggtcgccgg gaaaaattca ttgccctgat cccactcaat
    28741 agccactaat tcattaagga aaggtgctgc gcgatgtact ggattgtcag ccagatgcgg
    28801 gtaggccaca tgcccctgaa cgccatgaat ggtaaggttg caggttaacg atccgcgacg
    28861 accatttttc accacatcac caaccacttc gatactcgac ggttcgccga ccaggcagta
    28921 atcgagacgc tcattacgtg ccatcaacgc ttcgacgact tttaccgtac cgttgtgggc
    28981 actggcttct tcatcagagg tgatcagaaa tgccagtcgc cccgtatggt tgggatgttg
    29041 tgcgacaaag cgttctgccg ctaccaccat cgccgccagc gagcctttca tatctgccgc
    29101 accgcgtccg aataacatgc catcacgaat ggtgggttca aacggcggat tgatccaacg
    29161 atcggcgtcg ccaggcggca ccacgtcggt atgcccggca aaggccaacg tttcaccttg
    29221 cccacgccat gcccaaaaat tctgcgtatc ggcaaaatcc atgcgttcaa cggtaaaacc
    29281 gatcgcctgc aaacgttcaa tcaacaaagc ctggcatcct gcgtcatcag gactcaggga
    29341 agggcggcga ataagctgtt gtgtcagctc aataaccggg cacgacatag actacacctc
    29401 atggaaaaat tgctgataac tggaatcact gaaacccagc agcataggct taccaggcgc
    29461 gcagagcaat ggacgtttga taattgcagg catttcagtc attaatgccg ccgcagaggc
    29521 cgcatcggtg attttattgc gggtggtttc gtccagttta cgccaggttg taccacgggt
    29581 gttcagtaac gcttcccagc ctaattcgtt gataaaaccg ttcaataatt cgctgtccag
    29641 cccatcgacg cggtaatcat gaaaacgata gtcgatgtta ttggcttcca gccaacggcg
    29701 agccttttta atggtgtcac aatttttgat gccgtaaagt gtaaccataa tgaatccttt
    29761 ttcgatttat gagaataaat actcatttaa gggtgaaaac attttgcctg caaatgatta
    29821 tcaggaaata taacattatc gaagtat
//
LOCUS       DAPGWD010000044        29641 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPGWD010000044 DAPGWD010000000
VERSION     DAPGWD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.044"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="RHX88_001904"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="RHX88_001904"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDV2230596.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="RHX88_001905"
     CDS             complement(804..1352)
                     /locus_tag="RHX88_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDV2230597.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="RHX88_001906"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="RHX88_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV2230598.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQMTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="RHX88_001907"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="RHX88_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDV2230599.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="RHX88_001908"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="RHX88_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDV2230600.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="RHX88_001909"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="RHX88_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDV2230601.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="RHX88_001910"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="RHX88_001910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDV2230602.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="RHX88_001911"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="RHX88_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDV2230603.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /locus_tag="RHX88_001912"
     CDS             complement(11782..12675)
                     /locus_tag="RHX88_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDV2230604.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="RHX88_001913"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="RHX88_001913"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDV2230605.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="RHX88_001914"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="RHX88_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDV2230606.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /locus_tag="RHX88_001915"
     CDS             complement(13806..15038)
                     /locus_tag="RHX88_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDV2230607.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="RHX88_001916"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="RHX88_001916"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDV2230608.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="RHX88_001917"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="RHX88_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDV2230609.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /locus_tag="RHX88_001918"
     CDS             complement(17843..20107)
                     /locus_tag="RHX88_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDV2230610.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSVWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="RHX88_001919"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="RHX88_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDV2230611.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="RHX88_001920"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="RHX88_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDV2230612.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="RHX88_001921"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="RHX88_001921"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDV2230613.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="RHX88_001922"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="RHX88_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDV2230614.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="RHX88_001923"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="RHX88_001923"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDV2230615.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="RHX88_001924"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="RHX88_001924"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDV2230616.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIECPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /locus_tag="RHX88_001925"
     CDS             complement(26972..27664)
                     /locus_tag="RHX88_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDV2230617.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /locus_tag="RHX88_001926"
     CDS             27794..29554
                     /locus_tag="RHX88_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDV2230618.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7197 a         8065 c         7199 g         7180 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgtc acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtacgaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcac gaacggcagg cgcaggttta
     2461 ctacgagacg tcatttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtcctgca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgca gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgc tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccaaccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgtgcg ctgtcccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccaacg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttgagg cgcacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcacggga ggttaattct tcggtcagac
     4441 ggctaagttc gatctccatc tgttcgatag cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgct
     4561 ccggacgttt cggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gcgcaaatca tcagcggaga
     4681 gataagccag ctcacgacgg tgtaagcggc gctcaacgcc gttatctttc accatgcgca
     4741 tcaccgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcaggt tgtccatctc cgcttcgcag aaggtaagcg
     4861 ctttttccag ttgattgcgg cgtgaacggt tattgctcag ttgcgcatgc agctcgtcac
     4921 ggcgaatacg cgcccgctct tctgccccgc tatcagcacg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagctt ttcgttgaga tcgctattac
     5161 cgctcagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttagctg ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttactcaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaagag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 tcaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatctccttc gatcagatag agatcttccg ggcaatcggt caagccttcc aggtgttcag
     5941 ttacctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acaccaccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacggcg tttttgcgcg cgcccacttc atcgcgttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcgccgca ctgttcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagc tcgtcaatgt caaaattctt gccctgacgt ttgcagaaat
     6481 ctgccagcag acgctcagct tcttgctgtt cgcgcagacg ctgttccagt tcgcttaagc
     6541 gcatccgcaa cggctgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcaata
     6601 gttcgcgagc gacatcccac gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgctgtc ggcggttaag tccggcagat ggcacagttc tttggcgcga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 cagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgcg cgagcctcgt
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtaac tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat
     7321 gcttaaacag gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atgcggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcagtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgtgtcggct gcaccgacat cggcagtccc tgaatggcaa
     7741 acggcttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atggtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ctcttcggtt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc gaaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt tttgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg gctgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagtcc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tacgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacggc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc cagatcctca ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaacc gctgagcaaa gacgcggttt ttatccatat
     9241 cgatcgcggt acgaataaat ttgtgtacgt ggcggtcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccaactgata atacgatcga gtttgctctg cagatcgaac accagacgat
     9361 cgacgaaatg cagatcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaatttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtc ccggaagttt
     9481 ccgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 cgatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatctg ctgcgcgttt gagctcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttcac caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgtgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggaggtggtt atacctggaa cccacagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacgggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta acccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtgttattg aactgattga gcgtggtcca tagcacttca acatgttgcc ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagt tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcggggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac ggacttgcct gccagttgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggcctt ctgcaaaacg
    13081 atcacgatcc caaggaatgg tggcgcgaga gaagtacagt gcataccctt cagcgtcgag
    13141 aaccactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcaccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgattgtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca ggcgttctgt ccctgactgg tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcgacatcc tcatgatcgg ttgccacgat
    13441 gatgcgatcg gcacctgatt cgcgagcacg ttcaagaaca tgaacaatca tgggtttgcc
    13501 attaatatca accaatggtt taccgggcag acgcgttgac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcacg cagcgggaaa gccaggttgt ccagtttgca aataagttct
    13681 tgtttttctt ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 agacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tgctctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctgcgt
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgctcgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga
    15001 ttacgcgcat cagcaaggga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggt gcttaccaac
    15241 gcgctgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccgc acatcttcag cgtggcaaaa aagcgcggcg gatgcccaat ccctgccatc
    15361 gccaccacat gttcaagctg agcaacatca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagatgcat ggggatttca ccgctgcggg ggacaccgcc gttgacgatt
    15481 accgcatcaa ctgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atgatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc accatatccc cgcgatacga cccccacgcg aattccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc actcaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgccg cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt catccagaat cagaatcggg ctatcacgca
    16321 gcaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccatg gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agtccgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gtaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgagtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagtgat ttcagcggac
    16921 gcatcagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagctcgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcaaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc cttcagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt ccccgttgac tgtttgtcaa
    17461 aaaatgaaac cggcattccc atcatgtgac caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgaggc tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgtcac ttgaccctga tgtggagtat caagccattg atatccttgc tgttgatagc
    18001 gatgcttaac tttattagag ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gtctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtgtgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcggg ccaggccagt cctgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacctg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagcaagc
    18661 ctacagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacgttcagc aatgttgatc cacccttctg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcta atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacaggca taagcaggag tgtgattccc agttgcaaat
    19021 gaatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actggtacca aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccaga
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccac
    19321 caattaatgg catttgccag tggatccagc gcccaggcag ataaacttgc ccaccgcgaa
    19381 tgagtccggc agccaacaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcacgc atgatatttt tgatttcttt ggggacggat aaccgttccc
    19501 ccatacctaa accaagaata accgcattcc acatataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaagc
    19621 ggccggtgag cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttggcc atgaactgca cgaactttga gtttcattga ccacagttgt ccggcacaaa
    19741 ccgcttgcgg aagatattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac
    19801 cttgtagata agtaatttga ccgtaatgag tggtcatgcc gtcagtagca gtgatctcaa
    19861 caattgcatc ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg
    19921 ataatatgcc ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag
    19981 gtataaatgc cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaatttta cgttccccga ggcacgttca gaagaattta acgagagggt gaaaactgct
    20221 caaaactctg cgaggcagct tccagttcct gattagagaa atgaaaaaag cacccgatag
    20281 gtgcttttct cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaggcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc
    21661 cagagataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattactttg aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgctcaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gcctggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgta cgcgtgggaa tgccgcgact
    22921 tgtgaagcag cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggtcgac acaaaacgta catccagatg ggatgccagc
    23041 ggcaccagcg catcttccga tgcaacatca acatggtgat gtaaagccgc cagtgccagt
    23101 acgcgataaa ttgcacccga gtccagcaga tgccattgca acgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccgccatc cagactagcc agtttttcaa agctgccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt gatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagcagaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acgaacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgatg gccagatcgg tattaatacc gtgggtattt tgacaccccg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac gactgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccggtaatgt gccgacgttt tgataaagaa
    24301 agatatggct atttattgga cgttattcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttaggg tcaagaattg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt atgtcgcgat
    24481 ctcggcaaaa ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cttcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tctctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gcaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgcggctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaataactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca ctcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgcggt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agctgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtaaatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttcgccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggtaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 cgccacgctg atggctatct tcaaaaagga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggt gtctacgctt gccgcattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaagct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgttttttta atgccccgct ttacatatat
    29641 t
//
LOCUS       DAPGWD010000045        29512 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPGWD010000045 DAPGWD010000000
VERSION     DAPGWD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.045"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..2663
                     /gene="rhsB"
                     /locus_tag="RHX88_001927"
                     /pseudo
     CDS             1..2663
                     /gene="rhsB"
                     /locus_tag="RHX88_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
     gene            complement(2811..3791)
                     /locus_tag="RHX88_001928"
     CDS             complement(2811..3791)
                     /locus_tag="RHX88_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HDV2230619.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSH"
     gene            complement(4087..5223)
                     /locus_tag="RHX88_001929"
     CDS             complement(4087..5223)
                     /locus_tag="RHX88_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2230620.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5226..5588)
                     /locus_tag="RHX88_001930"
     CDS             complement(5226..5588)
                     /locus_tag="RHX88_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV2230621.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6125..8038
                     /gene="mtlA"
                     /locus_tag="RHX88_001931"
     CDS             6125..8038
                     /gene="mtlA"
                     /locus_tag="RHX88_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDV2230622.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8268..9416
                     /gene="mtlD"
                     /locus_tag="RHX88_001932"
     CDS             8268..9416
                     /gene="mtlD"
                     /locus_tag="RHX88_001932"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV2230623.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9416..10003
                     /gene="mtlR"
                     /locus_tag="RHX88_001933"
     CDS             9416..10003
                     /gene="mtlR"
                     /locus_tag="RHX88_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDV2230624.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(10015..10224)
                     /locus_tag="RHX88_001934"
     CDS             complement(10015..10224)
                     /locus_tag="RHX88_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230625.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10509..10871
                     /locus_tag="RHX88_001935"
     CDS             10509..10871
                     /locus_tag="RHX88_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDV2230626.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            11243..12898
                     /gene="lldP"
                     /locus_tag="RHX88_001936"
     CDS             11243..12898
                     /gene="lldP"
                     /locus_tag="RHX88_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDV2230627.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCIVGVITTLQAYVLTWMIP"
     gene            12898..13674
                     /gene="lldR"
                     /locus_tag="RHX88_001937"
     CDS             12898..13674
                     /gene="lldR"
                     /locus_tag="RHX88_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDV2230628.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            13671..14861
                     /gene="lldD"
                     /locus_tag="RHX88_001938"
     CDS             13671..14861
                     /gene="lldD"
                     /locus_tag="RHX88_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDV2230629.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            15059..15532
                     /gene="trmL"
                     /locus_tag="RHX88_001939"
     CDS             15059..15532
                     /gene="trmL"
                     /locus_tag="RHX88_001939"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDV2230630.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(15585..16406)
                     /gene="cysE"
                     /locus_tag="RHX88_001940"
     CDS             complement(15585..16406)
                     /gene="cysE"
                     /locus_tag="RHX88_001940"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDV2230631.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(16486..17505)
                     /gene="gpsA"
                     /locus_tag="RHX88_001941"
     CDS             complement(16486..17505)
                     /gene="gpsA"
                     /locus_tag="RHX88_001941"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDV2230632.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(17505..17972)
                     /gene="secB"
                     /locus_tag="RHX88_001942"
     CDS             complement(17505..17972)
                     /gene="secB"
                     /locus_tag="RHX88_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDV2230633.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18035..18286)
                     /gene="grxC"
                     /locus_tag="RHX88_001943"
     CDS             complement(18035..18286)
                     /gene="grxC"
                     /locus_tag="RHX88_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDV2230634.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(18428..18859)
                     /locus_tag="RHX88_001944"
     CDS             complement(18428..18859)
                     /locus_tag="RHX88_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDV2230635.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            19104..20648
                     /gene="gpmM"
                     /locus_tag="RHX88_001945"
                     /gene_synonym="pgmI"
     CDS             19104..20648
                     /gene="gpmM"
                     /locus_tag="RHX88_001945"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDV2230636.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            20658..21941
                     /gene="envC"
                     /locus_tag="RHX88_001946"
     CDS             20658..21941
                     /gene="envC"
                     /locus_tag="RHX88_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDV2230637.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            21945..22904
                     /locus_tag="RHX88_001947"
     CDS             21945..22904
                     /locus_tag="RHX88_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDV2230638.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(22891..23925)
                     /gene="waaH"
                     /locus_tag="RHX88_001948"
     CDS             complement(22891..23925)
                     /gene="waaH"
                     /locus_tag="RHX88_001948"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDV2230639.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(24164..25189)
                     /gene="tdh"
                     /locus_tag="RHX88_001949"
     CDS             complement(24164..25189)
                     /gene="tdh"
                     /locus_tag="RHX88_001949"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDV2230640.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFMIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(25199..26395)
                     /gene="kbl"
                     /locus_tag="RHX88_001950"
     CDS             complement(25199..26395)
                     /gene="kbl"
                     /locus_tag="RHX88_001950"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDV2230641.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPYLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            26609..27541
                     /gene="rfaD"
                     /locus_tag="RHX88_001951"
     CDS             26609..27541
                     /gene="rfaD"
                     /locus_tag="RHX88_001951"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDV2230642.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            27551..28597
                     /gene="rfaF"
                     /locus_tag="RHX88_001952"
     CDS             27551..28597
                     /gene="rfaF"
                     /locus_tag="RHX88_001952"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDV2230643.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            28601..>29512
                     /gene="rfaC"
                     /locus_tag="RHX88_001953"
     CDS             28601..>29512
                     /gene="rfaC"
                     /locus_tag="RHX88_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDV2230644.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPLMNL"
BASE COUNT         7098 a         7850 c         7838 g         6726 t
ORIGIN      
        1 gttacgacag cgacgggcgg gtgacagagc agcttaaccc ggcgggctta agctacacct
       61 actgttatga gcaaaaccgc atcaccatca ccgacagcct ggaccgccgt gaagtgctgc
      121 acacgcaggg cgaaggcggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg
      181 tcacgcagag tcagtttgat gcggtgggca ggcttaaggc acagacggat gccgcaggcc
      241 ggacaacaga atacagcccg gatgtggtga cgggactcat cacgcgcatc accacaccgg
      301 atggcagggc atcggcattt tactataacc accacagcca gttaacgtca gccaccgggc
      361 ctgacgggct ggaattgcgc cgggaatatg atgaatccgg ccgtctgatt caggaaaccg
      421 cccctgacgg cgatatcacc cgctaccgtt atgataatcc acacagtgac ttaccctgcg
      481 caacggacga tgccaccggc agccggaaaa ccatgacgtg gagccgttac ggccagttgc
      541 tgagcttcac cgactgttcc ggttatgtaa cccgttatga ccatgaccgt ttcgggcaga
      601 tgacggcggt gcaccgcgag gaagggctga gtcagtaccg cgcatacgac agccgtggac
      661 agttaattgc cgtgaaagac atgcagggcc atgaaatgcg gtatgaatac aacgccgccg
      721 gtgacctgac cgccgtcatt gctccggacg gcagcagaaa cgggacacag tacgatgcgt
      781 ggggaaaggc catccgtacc acgcagggcg ggctgacgcg cagtatggaa tacgatgctg
      841 ccggacgggt catccgcctg accagtgaaa acggcagcca taccaccttc cgttacgatg
      901 tactcgaccg gctgatacag gaaaccggct ttgacggccg cacacagcgt tatcaccacg
      961 acctgaccgg caaacttatc cgcagcgagg atgaggggct ggtcacccac tggcactatg
     1021 acgaagcaga ccgcctcacg caccgcaccg tgaatggcga aaccgcagag cggtggcagt
     1081 atgacgaacg cggctggctg acagacatca gccatatcag cgaagggcac cgggtgacgg
     1141 tgcattacgg gtatgatgag aaaggccggc tgaccggtga gcgtcagacg gtgcatcacc
     1201 cgcagacgga agcactgctc tggcagcatg agaccagaca tgcgtacaac gcgcagggac
     1261 tggcgaaccg ctgtataccg gacagcctgc ccgccgtgga atggctgacc tacggcagcg
     1321 gctggctggc aggcatgaag ctcggcgaca caccgctggt ggatttcacc cgcgaccgcc
     1381 tgcaccggga aacgctgcgc agcttcggcc gttatgaact caccaccgct tatacccctg
     1441 ccgggcagtt acagagccag cacctgaaca gcctgctgtc tgaccgcgat tacacctgga
     1501 acgacaacgg cgaactcatc cgcatcagca gcccgcgcca gacccggagt tacagctaca
     1561 gcaccaccgg caggctgacc ggcgttcaca ccaccgcagc gaatctggat atccgcatcc
     1621 cgtatgccac agacccggca ggtaaccgcc tgcccgaccc ggagctgcac ccggacagca
     1681 ccctcagcat gtggccggat aaccgtatcg cccgtgacgc gcactatctt taccggtatg
     1741 accgtcacgg caggctgaca gagaaaaccg acctcatccc ggaaggggtt atccgcacgg
     1801 atgatgagcg gactcaccgg taccattacg acagtcagca ccggctggtg cactacacgc
     1861 ggacacaata tgaagagccg ctggtcgaaa gtcgctatct ttacgacccg ctgggccgca
     1921 gggtggcaaa acgggtgtgg cggcgtgaac gggacctgac gggctggatg tcgctgtcac
     1981 ggaaaccgca agtgacctgg tacggctggg acggcgaccg gctgaccacg atacagaacg
     2041 acaggagccg catccagacg atttatcagc cggggagctt cacgccactc atcagggtcg
     2101 aaactgccac cggtgagctg gcgaaaacgc agcgccgcag cctggcggat gcccttcagc
     2161 agtccggcgg cgaagacggt ggcagtgtgg tgttcccgcc ggtgctggtg cagatgctcg
     2221 accagctgga aagtgaaatc ctggctgacc gggtgagtga ggaaagccgc cgctggctgg
     2281 catcgtgcgg cctgaccgtg gagcagatgc aaaaccagat ggacccggtg tacacgccgg
     2341 cgcgaaaaat ccgcctgtac cactgcgacc atcgcggcct gccgctggcc cttatcagca
     2401 aggaagggac aacagaatgg tgcgcagaat acgatgaatg gggcaacctg ctgaatgaag
     2461 agaacccgca tcagctgcag cagcttatcc gcctgccggg gcagcagtat gatgaggagt
     2521 ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcgg tatatcactc
     2581 aggatccgat tgggctgaag gggggatgga atttttatca gtatccgttg aatccagtta
     2641 cgaatacaga tcctctgggg ttagggaagg tgcgaataag cggggaaatt cttctcggct
     2701 gactcagtca tttcatttct tcatgtttga gccgattttt tctcccgtaa atgccttgaa
     2761 tcagcctatt tagaccgttt cttcgccatt taaggcgtta tccccagttt ttagtgagat
     2821 ctctcccact gacgtatcat ttggtccacc cgaaacaggt tggccagggt gaataacatc
     2881 gccagttggt tatcgttttt cagcagcccc ttgtatctgg ctttcacgaa gccgaactgc
     2941 cgcttgatga tgcgaaacgg gtgctctacc ttggcacgga tgctggcttt catgtattcg
     3001 atgttgatgg ccgttttgtt cttgcgcgga tgctgcttca aggtttttac cttgccggga
     3061 cgctcggcga tcagccagtc cacatccacc tcggccagct cctcgcgctg tggcgctcct
     3121 tggtagccgg catcggctga gacaaattgc tcctctccat gaagcagatt acccagctga
     3181 ttgaggtcat gctcgttggc cgcggtggtg actaggctgt gggtcaggcc actcttggca
     3241 tcgacaccaa tgtgggcctt catgccaaag tgccactgat tgcctttctt ggtctgatgc
     3301 atctccggat cgcgttgctg ctctttgttc ttggtagagc tgggtgcctc aatgatggtg
     3361 gcatccacca aagtgccttg ggtcatcatg acgcctgctt cggccagcca gcgattgatg
     3421 gtcttgaaca attgacgggc cagttgatgc tgctcgagca ggtggcggaa attcatgatg
     3481 gtggtgcgat ccggcagggc gctatccagg gataatcggg caaacaggcg catggaggcg
     3541 atttcgtaca gggcatcttc catggcaccg tcgctcaggt tgtaccaatg ctgcatgcag
     3601 tgaatacgca gcatggtctc cagcggatag ggccgtcggc cattgcccgc cttgggataa
     3661 aacggctcga tgacagcggt catattctgc catggcagaa tctgctccat gcgggagagg
     3721 aaaatctctt ttcgggtctg acggcgctta gtgctgaatt cactatcggc gaaggtgagt
     3781 tgatggctca tgatgtccct ctgggatgcg ctccggatga atatgatgat ctcatatcag
     3841 gaacttgttc gcaccttcct tagagccgtc gcaggaggaa caactcatat catcattgcg
     3901 atctcgacga tgtatagtct catcctgcgg cagaacaaga cggataaaaa atccaatgag
     3961 agcacactca tccagaactt attaacataa tgaatattaa agaaaaatca tcgcatctaa
     4021 tgaagtaata tgcaaaaata attagtcctt acaaggagca aagttccata atctcccccc
     4081 tccccctcaa tgatccaaat aaagataatg catccagctg gtcattctta gcagcacttt
     4141 ccgcatcacc gaaacatggc tgaaatggtc ggagtaaacc gccacctggg cgtagatgcc
     4201 gtcgggtaag gcatcgatat catcgttagg gtccagttca atggttccca gcacaccgtc
     4261 cgtgccgggc acgaccgtta atgattgcaa taccccctgc gcctgataag aaccgcctgg
     4321 cacgacaggt aaaatactag tcagtttgcc gtgaaacacc tgcccaggta gcgcgttaaa
     4381 caccacttcc gcatcatcgc cgggtttcag acgtaacagc gagttttgcc gaaattgggc
     4441 gacaatttgc cgtttttgct cggggatgaa gaccatcacc ggacgcagcg gcaaggcagc
     4501 tgcgtatgta cctgggcgga tcagtacctg agtaacgtag ccattgctcg gcgcgcgaat
     4561 gacagtctgc tcaaggttat attttgcttc agtaagttgc gctcttaagc tcacaatctg
     4621 cgattgctcg ccgttaacca tactgtcgag ctggctctgg atctgcgcct gctccgccac
     4681 cgagcctttc accagcgcat cctgcgcgag gaaattttgc cgcgcatcgt cgatgtcacg
     4741 ttccgagaac ggattcaccg ccgcctggct gcctttcaag taacgttgat aatttttaaa
     4801 cagacggtcg cgctccgctg aaacctgggt ggtgttggcc tgcgcttcag tgagctgcgc
     4861 acgcagcgtc tttatattat gcgtcgccgt catcaggtca gcctgaagtc tgtcaactcg
     4921 cgcctggtaa cgaaccgggt cgagcttaaa aagcacctcg cccttttgaa taagctgatt
     4981 attcttgtca gtgacttcag taacaattcc cgtcacctgt ggcgtgatag ggatcgctat
     5041 cactgccttt tgcgcggtaa aagtgtaagg gtggttgtag ttcatcaaca aaatcaaacc
     5101 actcaccaga aacacgcctc ccagcgccgc cgtcgccagc gtccactgat ttaccggaat
     5161 gcggaagatt ttaaagaccg cccacgccag cgccacgtaa gttaaaacaa tcaatagatc
     5221 cataattaga tctccgggaa cgtggaatag tcagtctttt tctcggcggc gagttgatgc
     5281 tccagccggg aaattcggtc agaaagtgcg gcgatttccg ggtcagtcgc tttctcctgc
     5341 gacgcaacat gtgactgcat accccagcca cgctccggtt gatagagcgt cgcccagatc
     5401 cacagaaacg gccagataac atgcagagta aacaggctca cccagcccgc cgtatgaata
     5461 gcgtcggcat ggggatggtt gcgctttttg gcaatcagat aagggatgtc atgtatcgcg
     5521 atgattccat aaaaaatcac cagaaagacg aagatcagca ctcccaacgc gaaatagttt
     5581 agaaacatat ctgcctcgga ttcacgttta tcagtgttgt ttttgggctg gcagccagaa
     5641 gggagtcagg ctgatatttt gacaataatc cgggttcgcg attctcgcca taacaccaaa
     5701 gaataatttt tagaggtgat gagttgctta gttacataac gattgtatga cgaaggcata
     5761 acatgctgta gatcacatca ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca
     5821 aagattcaac aaaccatcaa aacaaaaatg tgacactact cacatttaaa tgccattttt
     5881 agcgaaaatc gccgccttgt tgctttttta cacaagcgtt ttgtgatgaa cgtcacgtca
     5941 attacctctc taccccctat atttatgtga ttgatatcac acaaaaggcc gtcgactgga
     6001 cagttaaccg attcagtgcc agatttcgca gtatctacaa ggtccggcta cctctgccgc
     6061 cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt
     6121 ttttatgtca tccgatatta agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat
     6181 ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc atcaccgcgt tatttattcc
     6241 aacagggtgg ttaccgaacg agacgctggc gaagctggtc gggccgatga tcacttatct
     6301 cctgccgctg ctgatcggtt ataccggtgg taagctggta ggcggcgaac gtggcggcgt
     6361 agtcggtgcc atcaccacca tgggcgttat cgtcggcgca gacatgccga tgttcctcgg
     6421 ttctatgatt gcaggtccgc tgggcggctg gtgcattaag cacttcgacc gctgggtaga
     6481 cggtaagatc aaatccggtt ttgagatgct ggtgaataac ttctccgcag gcatcatcgg
     6541 gatgatcctc gctattctgg cattcctcgg cattggcccg attgttgaag ccctgtccaa
     6601 aatgctggct gcgggcgtta acttcatggt tgtccatgac atgctgccgc tggcgtctat
     6661 ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc attaaccacg gtatcttctc
     6721 gccgctgggt attcagcagt cccatgaact gggtaaatca atcttcttcc tgattgaagc
     6781 taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc
     6841 taaacagtct gcgggcggtg cggcaatcat ccacttcctg gggggtatcc acgaaatcta
     6901 cttcccgtat gtgctgatga atccgcgtct gatcctcgca gtcatcctcg gcggtatgac
     6961 tggcgtgttc acgctgacta tcctgggcgg tggtctggtt tctccggcat ctccgggttc
     7021 tatccttgct gtactggcga tgacaccaaa aggtgcttac ttcgctaaca tcgcgggtgt
     7081 gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt
     7141 gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg caggacatga aagctgagtc
     7201 taaaggcgca tctccgctgt ctgctggcga tgtgactaac gacctgagcc acgtacgtaa
     7261 aatcatcgtt gcctgtgacg ccggtatggg ttccagtgcg atgggcgcag gcgttctgcg
     7321 taagaaaatt caggatgcag gtctgtcgca gatttctgtt actaacagcg cgatcaacaa
     7381 cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg
     7441 ccaggttccg caggcacagc atatttcgct gaccaacttc ctcgacagcg gcctgtacac
     7501 cagcctgacc gaacgtctgg ttgctgccca acgccacacg gcaaacgaag agaaagtaaa
     7561 agacagcctg aaagacagct ttgacgattc cagtgctaac ctgttcaagc taggcgcgga
     7621 gaacatcttc ctcggtcgca aagcggcaac caaagaagaa gcgattcgtt ttgctggcga
     7681 gcagctggtg aaaggcggtt acgttgagcc ggaatacgtt caggcgatgc tggatcgtga
     7741 aaaactgacc ccgacttatc tgggtgagtc tatcgcggtg ccacacggta cggttgaagc
     7801 gaaagatcgc gtactgaaaa cgggcgtcgt gttctgccag tacccggaag gcgtgcgctt
     7861 cggtgaagaa gaagatgaca ttgcccgtct ggtgattggt attgctgccc gtaacaacga
     7921 gcacattcag gttatcacca gcctgaccaa tgcactggat gatgagtccg tcatcgagcg
     7981 tctggcacac accaccagcg tggatgaagt gctggaactg ctggcaggtc gtaagtaatc
     8041 caatcccacc ctctccacat ggagaaggtg gggttaattg cctgatgcgc tacgcttatc
     8101 aggcctacag gatgcatcac aatttgttga atttgcacgt tcttgtaggc cggataaggc
     8161 gcttacgccg catccggcgc tgcccctctc ctcacggaga gggtttgggt gagggaaaag
     8221 cctcacccca gccctctcgg gtaaaaacat tgatgaaggt taatactatg aaagcattac
     8281 attttggcgc aggtaatatc ggtcgtggct ttatcggtaa actgctggca gacgcgggta
     8341 tccaactgac gtttgccgat gtcaatcagg tggtacttga tgccctgaat gcccgtcata
     8401 gctatcaggt acatgtggtt ggtgaaaccg agcaggtaga taccgtttcc ggcgtcaatg
     8461 ctgtcagcag cattggtgat gatgtcgttg atctgattgc tcaggttgat ttagtcacta
     8521 ccgccgttgg cccggttgtg ctggaacgta ttgctccggc aatcgccaaa gggctggtga
     8581 aacgtaaaga acaaggtaat gaatccccgc tgaacatcat cgcctgtgaa aacatggtac
     8641 gcggtaccac gcagctgaaa ggccatgtga tgaacgccct gccggaagac gccaaagcgt
     8701 gggtagaaga acacgttggc tttgtcgatt ccgccgttga ccgcatcgta ccgccttcgg
     8761 cttcggcaac taacgatccg ctggaagtga cggtagaaac cttcagcgaa tggattgtcg
     8821 ataaaacgca gttcaaaggc gcactgccga acatcccagg catggagtta accgacaacc
     8881 tgatggcatt tgtcgaacgt aaactcttca ccctgaacac gggtcatgct ataaccgcgt
     8941 acctcggaaa actggccggt catcagacca ttcgtgacgc gattctcgac gagaaaatcc
     9001 gcgcggtggt aaaaggtgcg atggaagaaa gtggtgcagt attgatcaag cgctacggct
     9061 ttgacgctga caagcatgcg gcgtacatcc agaaaattct cggccgtttt gagaacccgt
     9121 atctgaaaga tgatgtagag cgcgtaggcc gtcagccact gcgtaaactg agtgctggcg
     9181 accgtctgat caagccactg ctcggtacgc tggaatatgg tctgccacat aaaaacctga
     9241 ttgaaggtat tgccgctgca atgcacttcc gcagtgaaga tgatccgcag gctcaggaac
     9301 tggcagcact gatcgctgac aaaggtccgc aggcggcgct ggcacagatt tccggtcttg
     9361 atgccaacag cgaggttgta tccgaggcgg taaccgctta taaagcaatg caataatggt
     9421 ggaccaggcg caggacaccc tgcgcccgaa taacagattg tcagatatgc aggcaacaat
     9481 ggaacaaacc caggcctttg aaaaccgtgt gcttgagcgt ctgaatgctg gcaaaaccgt
     9541 gcgaagcttt ctgatcaccg ccgtcgagct cctgaccgag gcggtaaatc ttctggtgct
     9601 tcaggtattc cgcaaagacg attacgcggt gaagtatgct gtagaaccgt tactcgacgg
     9661 cgatggtccg ctgggcgatc tttctgtgcg tttaaaactc atttacgggt tgggcgtcat
     9721 taaccgccag gaatacgaag atgcggaact gctgatggca ttgcgtgaag agctaaatca
     9781 cgacggcaac gagtacgcct ttaccgacga cgaaatcctt ggaccctttg gtgaactgca
     9841 ctgcgtggcg gcgttaccac cgccgccaca gtttgaacca gcagactcca gtttgtatgc
     9901 aatgcaaatt cagcgctatc aacaggctgt gcgatcaaca atggtccttt cactgactga
     9961 gctgatttcc aaaatcagct taaaaaaagc ctttcaaaag taagcaacgt ctgcttactg
    10021 cccctctacc tgcttcggcc gataaagccg acgataatac tccagacgtt gtagatataa
    10081 cggcacgctt tcatcgggta ttccggacgg aatcgcgtta cggggaggaa gttttttcag
    10141 atactcccgg aacgcctggc ttgatgccat gaaatctacg gctttatcga taagaagcgg
    10201 aacgttttca cctaatttgc ccatgatgat atctccgtat taccccgcgc cgggaatacg
    10261 cggccgccaa ttttagttta ggttcccggt aaagatgaca ttaaggaaaa cgtgctgaat
    10321 cctcaataat catccgctgc tgtttattat cttttttcta tagatttctt aattaatcaa
    10381 cgaattgcgt agtttttgag catgttaatg attacgttac agaactatta caatgtcatt
    10441 agttgtgaac aaagcacctg gtcgcgcata ctaggggcta taaatttatc tttatcagaa
    10501 gccatcacat gaaagaagtc gaaaaaaacg aaatcaaacg tctcagcgat cgcctggacg
    10561 ccatccgcca ccagcaggcc gatctttcgc tggttgaagc cgcagacaaa tatgccgagc
    10621 tggaaaaaga gaaagccacg ctggaagcag aaattgctcg cctgcgtgaa gttcatagcc
    10681 aaaaactgag taaagaagca cagaagctga tgaagatgcc attccagcgc gcaattacca
    10741 aaaaagagca ggctgatatg ggcaagctga agaaaagtgt tcgcggactg gtcgttgtgc
    10801 acccaatgac cgcactgggc cgcgaaatgg gcctgcagga gatgactggg ttttcaaaga
    10861 ccgcgtttta agaacacagt atctacaggg tgattctgca cattcctata ggccgagtaa
    10921 ggtgttcacg ccgcatccgg caagataagg cgctctggat caacaaccta agggcaattc
    10981 tctgatgagg attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc
    11041 aattcttcgc ttatctgacc tctggttcac aatttcccaa ttaaaactca catcaatgtt
    11101 gccaatacat aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc
    11161 agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg
    11221 cgtggactcc aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa
    11281 tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
    11341 taagctcaaa ctgaaaggat acgtcgccgc ctcgtggacg gtggcaatcg cccttgccgt
    11401 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggttt
    11461 cttctacggg ttgtggccca tcgcgtggat cattattgca gcggtgttcg tctataagat
    11521 ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga
    11581 ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gcgttccttg aaggagccgc
    11641 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc
    11701 gctgtacgcc gccgggctgt gcctgattgt taacaccgcg ccagtggcat ttggtgcgat
    11761 gggcattcca atcctggttg ccggacaggt aacaggtatc gacagctttg agattggtca
    11821 gatggtgggg cggcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat
    11881 tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg cgggcggctc
    11941 gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat
    12001 ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc agccagtgcg
    12061 tgtattccgt tttggtgatt tgggggcgtc acaggttgat atgacgctgg cccacaccgg
    12121 ttacactgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga cagctaccgt
    12181 aacactgtgg agtatcccgc cgtttaaagc cctgttcgca tcgggtggcg cgctgtatga
    12241 gtgggtgatc aatattccgg tgccgtacct cgataaactg gttgcccgta tgccgccagt
    12301 ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg
    12361 caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga
    12421 cgctatcagc accttcggca gcacgctgaa agaactggct ctgcccatct actccatcgg
    12481 tatggtgctg gcattcgcct ttatttcgaa ctattccgga ctgtcatcaa cactggcgct
    12541 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg
    12601 ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac
    12661 cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg
    12721 cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct
    12781 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
    12841 tatagtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg
    12901 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
    12961 gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc
    13021 ggcgtatcac gtaattcact gcgcgaggcg ctggcaaaac tggtgagtga aggcgtgctg
    13081 ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
    13141 aacatcgtcc agccgctaaa aacactgatg gccgatgatc cggattacag tttcgatatt
    13201 ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca
    13261 cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
    13321 atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
    13381 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc
    13441 cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct
    13501 gtcattgacg ccatttttgc cggtgatgct gacggggcgc gtaaagcaat gatggcgcac
    13561 cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg
    13621 attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt
    13681 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
    13741 atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
    13801 aagtggcgct gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc
    13861 tgtttaatga gaaattgtcg atgccggtgg cactggctcc ggtgggtttg tgtggcatgt
    13921 atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggt attccgttta
    13981 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa
    14041 tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag
    14101 caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac
    14161 gctaccgtga tgcgcattca ggtatgagcg gcccgaacgc ggcaatgcgc cgctacttgc
    14221 aagcggtgac acatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt
    14281 taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc
    14341 tggggaataa cttcgatccg tccatctcat ggaaagacct tgaatggatc cgcgatttct
    14401 gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac
    14461 gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac
    14521 tctcttccgc ccgtgcactg cctgctattg cagatgcggt gaaaggtgat atagccattc
    14581 tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg
    14641 acaccgtact gctgggtcgt gctttcttgt atgcgctggc aacagcgggc caggcgggtg
    14701 tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
    14761 cgaaatcgat cagcgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc
    14821 ctgcggcact ggctcccatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc
    14881 ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
    14941 aacgacaccg cactcttttt ttctccctcg cccctccggg gagagggccg gggtgagggg
    15001 aaaaggccgc actgctcacc aattttctgc tatcctgccc ccgcactaag ggggcagtat
    15061 gctaaacatc gtactttacg aaccagaaat tccgccaaat actggcaaca tcatccgtct
    15121 ttgcgctaat accggctttc gtctgcatat catcgaaccg atgggatttg cctgggacga
    15181 taagcgcctg cgccgcgcgg ggctggacta tcacgagttt accgccgtta cgcgtcatca
    15241 tgactatcgc gcgttcctcg aagcagaaaa tccccagcgc ctgttcgccc tcaccacgaa
    15301 aggtacacct gctcacagcg ccgtaagcta tcaggatggc gattatctga tgttcggccc
    15361 ggaaacacgc ggcctgccag cgagcattct tgatgccctg cccgctgaac aaaaaattcg
    15421 cattccgatg gtgccggaca gccgcagcat gaatctgtcc aatgcggtgt cggtagtggt
    15481 gtatgaagcc tggcggcagt tggggtatcc gggagcggta ttgagagatt agttactgta
    15541 ggccggatag atgattacat cgcatccggc acgatcacag gacattagat cccatcccca
    15601 tactcaaatg tatggttaat accgttgaaa tgctggtcca tatccattga tggcttatcg
    15661 ctgtctggtt taccgacaat acgagccgga acgccagcgg cggtggtatg cggcggcacc
    15721 ggttgcagca ccacggaacc tgcgccaatc ttcgcgccgc gcccaacttc aatattgccg
    15781 aggattttcg cgcccgcgcc aatcatcaca ccttcacgaa ttttcgggtg acggtcacca
    15841 ccagatttac ccgtaccgcc aagcgtcaca gattgcagaa tcgatacgtc gttttcaatc
    15901 accgccgttt caccaacgac gatgcctgtc gcgtggtcaa gcatgatacc gcgaccaatt
    15961 tttgctgccg ggtgaatatc gacctggaac gtcacagaaa cctggttttg cagaaagatt
    16021 gccagtgcgc gacgcccctg attccacaac cagtgaccga tgcgataggc ctgcaaggca
    16081 tgaaaaccct tcaggtataa caacggggtt gagtatttat cgactgccgg gtcgcgggta
    16141 cgcaccgcct gaatatcaca ggccgcagag gcgatcattt ccgggtcagc ggcgtaggct
    16201 tcttccacca cttcacggat agcaatagca ggcataattg gcgatgacag cttgttcgcc
    16261 agcatgtagc tcagtgcact gccaaggttt tcgtgtttga gtagcgtcgc gtggtaaaaa
    16321 ctggccagca ttggctcaca gtccgccagc gttctggctt cggctttaat attgttccag
    16381 acaatttcca gttcttcaca cgacattgct tactccacac gatgagataa tgaccggccc
    16441 gttctgcgcg ggccgggtca tagcggtaac aaaggttccc tgaggttagt ggctgctgcg
    16501 ctcgtccttg cgtgcacgac ctaataacgt caatgctgcc tcgcgcgcgt tttttccgca
    16561 atataatact tgataaattt cctcggttat tggcatttca acgccgaagc gatgcgccag
    16621 ttcgcggact tctttcgtat tgcggtagcc ttccaccacc tgaccaatct tctcctgcgc
    16681 gctttgtaca tccatgccct gaccgagcat catgccaaaa cggcggttac gcgactggtt
    16741 gtcggtacag gtaagcacca gatcccccag ccccgccatg cccataaagg tggcaggatc
    16801 ggcacccagc gccgcgccaa gacgcgacat ttcagccagc ccacgggtga tcagcgccgt
    16861 acgcgcattc gcaccaaaac cgataccgtc ggacatcccc gcaccaatgg caatgacgtt
    16921 tttcaccgcg ccgccaagct gcacgccaat gaaatccggg ttactgtaaa cgcggaaact
    16981 tttgccgcag tgcagcagct gctgaagatc atcagcaaag gtctgatcgg tcgaggccag
    17041 cgaaatagct gtcggtaaac ctgccgccag ttctttcgca aacgttgggc cagagataac
    17101 cgccagcgga atttgatcgc ctaacgcctc gcgggccacg tcctgtaaca gacgcccggt
    17161 ttccgcttcc agccctttgg tcgcccacac cagacgcgca tcaggacgca tcagcggttt
    17221 aatctggcgc agcacttcac caaagacatg gctgggtacg acgacgagaa tattacggct
    17281 ggctgccagc gcagtggcga gatcgctttc aagatggagc gtatcgggaa aaggcacatc
    17341 ggggagaaac gcggcgttac agcggtcgcg ttcaagcgtt gcgatatgtt cagggtcatg
    17401 gccccagagg acaacctcgt ggccatttct tgccagggta atggcaagag cggtgccgta
    17461 cgagccggca ccgatcacag tcattgaagc attacgttgg ttcatcaggc atcctgatgt
    17521 tcttcagtac cttcgccagc ctgctgctgc aaatagttca tgaacagcgc atcgaagtta
    17581 accggcgcaa ggttcagttg cgggaatgta ccgcgggata ccatgctggt gatgcactca
    17641 cgagcatacg ggaacagaat gttcgggcag tatgcgccca ggcaatgtgc catctgggtg
    17701 ccttcgatac ccgcgatgga gaaaataccg ccctgctgaa cttcacacag gaacgcggtt
    17761 tcttcgccca gggaggccgt tacggtaaca cgcagtacca cttcgtatac gtcatctgcc
    17821 agttgggaag atgccgtatc cagatcaagt ttaacttctg gttgccaatc tttctggaaa
    17881 acgtgcggcg cgttcggcgc ttcgaaagag atatccttgg tataaatacg ttggatctgg
    17941 aaagtcattt cagtgttgtt ttgttctgac atgtgtagaa aacccttaag tgttgtcctt
    18001 aaatactgcg caatgtaatg ccaacgttca cacgttattt cagcagggga tccagtccac
    18061 cacgtgcatc caatgcatac aagtcatcac agccgccaat gtgctgtgcg tcaataaaaa
    18121 tctggggaac cgtggtgcga ccgctgcgtt tgatcatctc ttcacgcttg gcggcattgc
    18181 catcgatcgg caactcctga aaactcacgc ccttgctgct cagcagtgct tttgcacgat
    18241 ggcaatacgg gcaggtttct ttggtataga tttcaacatt ggccataatt tagctcctgt
    18301 ttttttaccc tgaaatttca tgttgcaggg cggctgcaaa atggagtgtc cccagaagct
    18361 tataacaata agtcgctggg ggcctaattg cagctaacgg cctgcatcat gaaagacgac
    18421 aggtaaatta tttgccgcgc accagaggca agttttcgcc agcccagcca gcaacgccct
    18481 ctttcagcac gaatacttgc gcaaaaccag ctttcgtcag cgcgtttgca ggctcctggc
    18541 actgcatgcc agaaccgtct accacgataa ccggtttgtc tttgtgcttc tcaagctcac
    18601 caacattgtt ggctttgatt tcgctcggca acagattgat agaacctgcg atatggcctt
    18661 tacggaagtc atcacgctga cgtaaatcca caaccacagc gtcttctttg ttgatcagac
    18721 gcgtagcttc accacgagta atcaccttca ctttcgaggt caggctctta aacgtagtca
    18781 caagaaccgc caccagtaac gcgatccagg cgatactcag tatgggatga cggccaacaa
    18841 attgcataat ttcttgcatg gggggtaaca actcccgacg tagtgattaa aaaaaccagg
    18901 aaaggagtat acctgcgcgg tgcggcaaat acagccagcg cgttaactgg aatgcaattt
    18961 tgcggggcac gacgaaaaaa aagcgcaaat ctggcatcgg cttgccgcga gcggtcgtat
    19021 attttgatct tcagaggcta ttttatcgat tcagctgtag taaaattacg caaattttga
    19081 ctcttgagta tgaggttgtc gcaatgtcgg tttctaaaaa acctatggta ctggtgattc
    19141 tggatggcta tggctatcgt gaagaacagc aggataacgc catttttagt gctaaaaccc
    19201 cggtaatgga tgcactgtgg gccaatcgtc cgcataccct gatcgacgct tccggtctgg
    19261 aagtcggtct gcctgaccgt cagatgggta actccgaagt aggccacgtt aacctgggtg
    19321 ccggccgcat cgtgtatcag gacctgactc gtctggacgt tgaaatcaaa gatcgcgctt
    19381 tctttgctaa tccagtgctg actggcgcgg tagataaagc gaaaaacgca ggtaaagcag
    19441 tacacattat gggtctgctc tccgcaggcg gcgtacacag ccacgaagat cacatcatgg
    19501 cgatggtaga actggcagct gaacgcggcg cagaaaaaat ctacctgcac gcattccttg
    19561 acggtcgcga caccccgccg cgcagtgctg aatcctcgct gaaaaaattt gaagagaaat
    19621 ttgctgcgct gggtaaaggc cgcgtagcgt ccatcattgg tcgttactac gcgatggacc
    19681 gcgataaccg ttgggatcgc gttgaaaaag cctatgacct gctgactctg gcgcagggtg
    19741 agttccaggc cgatactgcc gttgctggtt tgcaggcagc ttatgctcgc gacgaaaacg
    19801 acgagttcgt gaaagcgacc gttattcgtg cagaaggtca gccagatgcg gcaatggaag
    19861 acggcgatgc gctgattttc atgaacttcc gtgctgaccg cgcgcgcgaa atcactcgtg
    19921 cattcgtaaa cgcagatttc gacggcttcg cgcgtaagaa agtggttaac gtcgatttcg
    19981 tgatgctgac cgaatacgcc gctgacatca aaactgcggt tgcttaccca cccgcttccc
    20041 tggttaacac cttcggcgag tggatggcga aaaacgacaa aactcagttg cgtatttccg
    20101 aaaccgaaaa atatgcccac gttactttct tcttcaacgg tggcgtagaa gagtcgttca
    20161 aaggcgaaga tcgcattctg atcaactcac cgaaagtggc tacctacgat ctgcaaccgg
    20221 aaatgagctc cgcagagctg accgaaaaac tggttgcggc catcaagagc ggcaaatacg
    20281 acaccattat ctgtaactat ccgaacggcg acatggtagg tcacaccggg gtgatggaag
    20341 cggcggttaa agcggttgaa gcactggatc actgcgtgga agaagtcgcg aaagcggttg
    20401 agtccgtggg tggacaactg ctgatcaccg ctgaccacgg taacgctgaa cagatgcgcg
    20461 atccggcaac gggtcaggca cacacggcac acaccaacct gccagttccg ctgatttacg
    20521 ttggtgataa gaacgtgaaa gcggttgcag gcggcaaact ttctgacatc gcgccgacca
    20581 tgttgtcgct gatgggtatg gaaatcccgc aagagatgac tggtaagccg ctgttcatcg
    20641 tggaataatc cctccccatg aggggaaagg cgattaatac catgacacgg gccgtgaaac
    20701 cgcgcaggtt tgcaatcagg cccatcatct acgccagcgt tcttagcgct ggcgtattgt
    20761 tgtgcgcctt ttccgcccac gcggatgagc gtgaccaact caaatctatt caggccgata
    20821 tcgccgcgaa agagcgcgcg gtacgccaaa agcaacaaca acgcgcaagc ctgctcgcac
    20881 aattgaaaaa gcaggaagaa gcgatctctg aagccacccg taagctgcgc gaaacgcaaa
    20941 acacgctcaa tcaactcaat aaacagattg atgagatgaa cgcgtcgatt gccaaactgg
    21001 agcagcaaaa agccgcccag gagcgcagcc tcgccgcaca actggatgcc gcattccgtc
    21061 agggcgagca taccggtatt cagctaattc tcagcggtga agaaagccag cgtggacagc
    21121 gtttacaggc ttatttcggc tatctcaacc aggcgcgaca agaaaccatt gcccagttga
    21181 agcaaacgcg tgaagaagtc gccatgcagc gtgctgaact ggaagagaaa caaagcgagc
    21241 aacaaacgct gttatatgaa cagcgcgccc aacaggcgaa actgactcag gctctgaacg
    21301 agcgtaaaaa gacgctggcg gggctggaat cttccatcca gcaaggtcag cagcagctaa
    21361 gtgaactgcg tgccaacgaa tcccgtctgc gtaacagcat tgcccgtgcg gaagccgcgg
    21421 cgaaagcgcg tgctgaacga gaagcgcgtg aggcccaggc ggttcgcgac cgccagaaag
    21481 aagcgacgcg taaaggcacc acctacaaac cgactgaaag cgaaaaatcg ctgatgtccc
    21541 gtaccggtgg tctgggtgcg ccgcgtggtc aggcattctg gccggttcgc gggccgacgc
    21601 tgcatcgcta tggtgaacag ctacagggcg aactacgctg gaaaggaatg gttatcggtg
    21661 cctctgaagg tactgaagtt aaagcgattg ccgacggtcg ggtgattctg gctgactggc
    21721 tgcaaggtta cggtctggtg gtggtggttg agcacggtaa aggcgacatg agtctttacg
    21781 gctataatca gagcgcactg gtgagcgttg gttcgcaggt tcgcgcgggc cagccaattg
    21841 cactggtggg cagcagtggc ggtcagggtc ggccttcact ctatttcgaa attcgccgcc
    21901 agggtcaggc ggtcaatcca cagccgtggt tgggaagata agttttgttt ccatttcgtc
    21961 gtaacgttct tgcatttgcc gctctgttgg cgctctcctc ccccgtactt gctggcaaac
    22021 ttgccatcgt cattgatgat tttgggtatc gcccgcacaa cgaaaaccag gtgctggcga
    22081 tgccctccgc tatctccgtc gctgtattac ccgattcacc gcacgccaga gagatggcga
    22141 ccaaagcgca taacagcggg cacgaagtgt tgattcatct cccgatggcg ccactgagta
    22201 aacagccgct ggagaaaaat acgctgcgcc cggagatgag cagcgacgaa attgagcgca
    22261 ttattcgtag tgcggtcaat aacgtgccct atgccgtggg gatcaacaac cacatgggta
    22321 gcaagatgac ctctaacctg tttggtatgc agaaagtgat gcaggcactg gagcgttaca
    22381 atctttactt cctcgacagc gtaaccatcg gtaataccca ggcgatgcgc gccgcgcaag
    22441 gtactggcgt gaaggtgatc aaacggaagg tgttcctcga cgattcgcag aacgaagcgg
    22501 acatccgtgt gcaatttaat cgcgcaattg atctggcgcg tcgcaacggt tcaaccattg
    22561 ccattgggca tcctcaccct tcaacggtac gcgtgctaca acagatggtt tataacctgc
    22621 cgccagacat tacgctggtg aaagccagca gcttgctgaa tgaaccgcag gttgatactt
    22681 ctacaccgcc gaaaaacgct gtgcctgatg caccgcgtaa tccgttccgt ggcgtgaagc
    22741 tgtgcaaacc gaagaaaccg atagaacctg tttatgctaa tcgcttcttt gaagtattaa
    22801 gcgaaagcat cagccagagc acgctgatcg tttacttcca gcatcagtgg caaggctggg
    22861 gcaaacagcc cgaagcggcg aagtttaacg ctagcgcaaa ttaaaggcgc tacgcgtaat
    22921 gcgatggtgc gaccgcgttt tgtcgcgcca ttgccataag cggaaagacc acagtaacgc
    22981 ctggtaaccg accttcacgc tgcgcacatt ggtaatcagg cgcttataca taccggaagt
    23041 aaatatctcg gcaatcatcc gttggcgggt aagaatatcc ggctctttgc gcaccgcatg
    23101 gcaaacacgc aatgcttcgt aagttatttg ctgatgaaat tccggataaa tcgtaatttt
    23161 gtcggcataa tttcgattta atttctccag caggcgggta atcttaatat agtgacgttg
    23221 ataattaagg tttttattcc cttgtctatg caaccgactc actgacgtat tatgcagata
    23281 atatttatat aatgattgct cggtatatcg cgcacgcagc gcgttaaaca tgaattctgt
    23341 tgtccagaca atatcctgat gatgtaatcc ggcaataaat ttaatgttat ttttaacaat
    23401 aacatcacgg cgataaaccc ccatccagac aacgtgagtc caacggcgcg aagaaagccc
    23461 catccgcagc cagtccgggc cggttaatac gccggttgag cgaaggcgat cgctggggat
    23521 ggattgccag gtttctcccg tttcacgaaa acaccagtca gcgttgcact gcgccacgtc
    23581 gaggtcatcc tctaacgcca tggtcatcag cgtttcgtac atggtgggat agacctcatc
    23641 gtcagcatcg acaaaagcga catatttgcc cgtcgccacc tcgatcccac gattacgcgc
    23701 taccgatgcg ccagcattcg cctgatgcaa caaacgaacg tgcggatagt tttctgcgta
    23761 atgctttgct atttcaacag aattatccgt tgaaccatcg ttaataataa tgatttccag
    23821 agcagtccag gtttgcgtaa ttaaagattc catacaagtg cggaaatcat cgcccgcatt
    23881 atataacgga ataataacac taagtttatt ggtgctgttc atcataaatt accgacgacc
    23941 tctgaagtta tcctgacgtt ttacaggctt tgtattaaga aactattaag cctgtgagat
    24001 attattttta caggtgagag agtcacttca gggtagagag ataatgagag cagctatata
    24061 agagatcagc gcaattacga tatctttatt acactgacag gattttacat atttaacgga
    24121 ttatcctcag gcaaaagggc tggaatacca gcccttgttc gtgttaatcc caactcagaa
    24181 ttactttccc ggactggccc gaacgcatag cgtcaaagcc cttctggaaa tcatcgatag
    24241 agaaacgatg ggtaatgatc ggcgagagat ccagacctga ctgaatcagc gccgccatct
    24301 tgtaccaggt ttcaaacatc tcacgaccgt aaataccttt aatgaacaag cctttaaaga
    24361 ttactttggt ccagtcgata gacatatcgg acggtggaat acccagcatc gcaatgcgac
    24421 cgccgtggtt catggtgtcg agcatggtac gaaacgctgg cggcgcaccg gacatttcca
    24481 gaccgacatc aaagccttcg gtcatgccca gttctgccat cacatcatta aggttttctt
    24541 tagcgacgtt gaccgcacgg gtgataccca ttttgcgcgc cagttcgaga cgatattcgt
    24601 taacatcagt gatcaccaca ttgcgtgcac caacgtgttt cgccaccgcc gctgccataa
    24661 taccaatcgg gcctgcacca gagaccagca catcctcgcc aaccagatcg aacgacagcg
    24721 cggtatgcac ggcgttaccg aagggatcaa aaatggaagc caggtcgtcg gagatattgt
    24781 cgggaatttt gaaggcgttg aacgccggaa tcaccagata ttcggcgaag cagcccgggc
    24841 ggttaacgcc gacgccgatc gtgttgcggc acagatgggt acgaccgcca cgacagttgc
    24901 gacagtgacc gcaggtgata tggccttcgc cagaaacgcg atcgccaatc atgaagccct
    24961 tcacttcctg accaatacct accacttcac cgacatattc atggcccacg accatcggca
    25021 ccgggatggt tttttgcgac cactcatccc agttatagat gtgaacgtca gtcccgcaga
    25081 tggctgtttt acggatttta atcagcagat cgttatgccc gagttccggt acaggaacgt
    25141 cggtcatcca gatgccctct tccgctttca gtttggataa cgctttcatc tcacatcctc
    25201 aggcgataac gcccagttgt ttaccaatac gcgtgaatgc ttctactgca cgcgtaattt
    25261 gctcaggggt atgcgccgca gacatctggg tacgaatacg cgcctgacct ttcggaacga
    25321 ccggatagaa gaaaccggtg acgtaaatgc cctctttttg cagctctcgg gcaaatttct
    25381 gcgccactac cgcatcacca agcatgaccg gaataatggc gtgatcggct cccgccaggg
    25441 taaagcccgc cgccgacatt tgctcacgga actgacgcgc gttcgcccac agacggtcac
    25501 gcagttcgct gcccgcttcg accatctcca gtactttgat ggacgcggca acaatggccg
    25561 gtgccagcga gttggagaac aggtacggac gagaacgctg gcgcagccac tcaaccacct
    25621 ctttgcgcgc cgcggtataa ccaccagaag ccccgcccag cgctttacca agtgtgccgg
    25681 tgataatatc gacccggccc atcacatcgc agtattcatg ggaaccacga ccattttcac
    25741 cgacaaaacc gaccgcgtgg gagtcatcca ccatcaccag ggcatcgtat ttatctgcca
    25801 gatcgcaaac gcctttcagg ttggcaatca cgccgtccat tgagaacaca ccatcggtgg
    25861 caatcagcac atgacgcgca ccggcttcac gcgcttcttt cagacgtgct tccagctcct
    25921 gcatatcgtt gttggcatag cgatagcgtt tagctttgca cagacgcaca ccatcaataa
    25981 tagacgcgtg gttcagtgcg tcggagataa tggcgtcttc cgcacccagc agcgtttcaa
    26041 acagtccacc gttagcatca aagcaggaag agtagagaat cgcatcttcc atccccagga
    26101 atgccgccag tttttgttca agctctttat ggctgtcctg agtgccgcaa ataaaacgca
    26161 ccgaagccat gccgaaaccg tgagaatcca ttcccgcctt tgccgccgca atcagatacg
    26221 gatgattcgc cagcccgaga tagttattgg cacaaaagtt aatgacgtgg cttccatcag
    26281 ccacagtgat atctgcttgc tgcgcagacg taataatgcg ctcttcttta aacaaccctt
    26341 ccgcccgtgc ggtttccaga tcgttggtta actgctgata aaattctcca cgcattgcga
    26401 ttctccagac agggcaaatt ccagcacata ttacccaaac ttataggtcg cgacgagatg
    26461 acacgttaac acttctgcaa aattcaggat aaataacgtc catcaatctc acttttggcg
    26521 aatggctgaa gccaacgccg tgtagtgata tgatagaagt actcgtgtct gagattgtct
    26581 ctgactccat aattcgaagg ttacagttat gatcatcgtt accggcggcg cgggctttat
    26641 cggcagcaac atcgttaaag ccctgaatga taaaggcatc accgatattc tggtggtgga
    26701 caacctgaaa gacggcacca agtttgtgaa cctggtggat ctgaatatcg cagactatat
    26761 ggataaggaa gacttcctga tccagattat ggctggcgaa gagttcggcg atgtcgaagc
    26821 gattttccac gaaggcgcgt gctcttccac caccgagtgg gacggcaagt atatgatgga
    26881 taacaactat caatactcca aggagctgct gcactactgc ctggagcgtg aaatcccgtt
    26941 cctgtacgct tcttccgcag ccacctacgg cggacgcacc tccgacttta ttgaatcccg
    27001 cgagtacgaa aaaccgttga acgtctacgg ttactcaaaa ttcctgtttg atgaatatgt
    27061 tcgccaaatc ctgccggaag cgaactcgca gattgttggc ttccgctatt tcaacgttta
    27121 tggaccgcgt gaaggccata aaggcagcat ggcgagcgtc gctttccatc tcaacaccca
    27181 gcttaataac ggtgaatcac cgaagctgtt cgaaggtagc gagaacttta aacgcgactt
    27241 cgtctatgtt ggcgatgtgg cagatgtaaa cctgtggttc ctggaaaatg gcgtttccgg
    27301 catcttcaac ctcggcaccg gtcgtgcgga atccttccag gctgtagctg atgctacgct
    27361 ggcttatcac aagaaaggcc agatcgaata cattccgttc ccggataagc tgaaaggccg
    27421 ctaccaggcg ttcactcagg cagatctgac aaatctgcgc gcggcgggtt acgacaaacc
    27481 gttcaaaacc gttgctgaag gtgtaacgga atacatggcc tggctgaatc gcgacgcata
    27541 agagctctgc atgaaaatac tggtgatcgg cccgtcttgg gttggcgaca tgatgatgtc
    27601 gcaaagtctc tatcgcacgc tccaggcgcg ctatccccag gcgataatcg atgtgatggc
    27661 accggcatgg tgccgtccat tattatcgcg gatgccggaa gttaacgaag ctatccctat
    27721 gcctctcggt cacggagcgc tggaaatcgg cgaacgccgc aaactgggtc atagcctgcg
    27781 tgaaaagcgc tacgaccgcg cctacgtctt acccaactcc ttcaaatccg cattagtgcc
    27841 tttcttcgcg ggtattcctc atcgcaccgg ctggcgcggc gagatgcgct acggtttact
    27901 caacgatgta cgcgtgctcg ataaagaagc ctggccgcta atggtggaac gctatgtcgc
    27961 gctggcctat gacaaaggca ttatgcgcac agcacaagat ctgccgcagc cattgttatg
    28021 gccgcagttg caggtgagcg aaggtgaaaa atcatatacc tgtaatcaat tttcgctttc
    28081 atcagaacgt ccgatgattg gtttttgccc gggtgcggag tttggtccgg caaaacgctg
    28141 gccacactac cactatgcgg agctggcaaa gcagctgatt gatgaaggtt atcaggtggt
    28201 tctgtttggc tcggcgaaag atcatgaagc aggcaatgag attcttgccg ctttgaatac
    28261 cgagcagcag gcatggtgtc ggaacctggc gggggaaaca cagcttgatc aagcggttat
    28321 cctgattgca gcctgtaaag ccattgtcac taacgattct ggcctgatgc acgttgcggc
    28381 ggcgctcaat cgtccgctgg ttgccctgta tggtccgagt agcccggact tcacaccgcc
    28441 gctatcccat aaagcgcgcg tgatccgcct gattaccggc tatcacaaag tgcgtaaagg
    28501 tgacgctgcg gagggttatc accagagctt gatcgacatt actccccagc gcgtactgga
    28561 agaactcaac gcgctattgt tacaagagga agcctgacgg atgcgggttt tgatcgttaa
    28621 aacatcgtcg atgggcgatg ttctccatac gttgcccgca ctcactgatg cccagcaggc
    28681 aatcccagaa attaagtttg actgggtggt ggaagaaggg ttcgcacaga ttccttcctg
    28741 gcacgctgcc gttgagcgag ttattcctgt cgcaatacgt cgctggcgta aagcctggtt
    28801 ctcggcccct ataaaagctg aacgcaaagc gtttcgtgaa gcgctacaag cagagaacta
    28861 tgacgcagtt atcgacgctc aggggctggt aaaaagcgcg gcgctggtga cgcgtctggc
    28921 gcatggcgta aagcatggca tggactggca aaccgctcgc gaacctttag ccagcctgtt
    28981 ttacaatcgt aagcatcata ttgcaaaaca gcagcacgcc gtagaacgca cccgcgaact
    29041 gtttgccaaa agtttgggct atagcaaacc gcaaacccag ggcgattatg ctatcgcaca
    29101 gcattttctg acgaacctgc ctacagatgc tggcgaatat gccgtatttc ttcatgcgac
    29161 gacccgtgat gataaacact ggccggaaga acactggcga gaattgattg gtttactggc
    29221 tgattcagga atacggatta aacttccgtg gggcgcgccg catgaggaag aacgggcgaa
    29281 acgactggcg gaaggatttg cttatgttga agtattgccg aagatgagtc tggaaggcgt
    29341 tgcccgcgtg ctggccgggg ctaaatttgt agtgtcggtg gatacggggt taagccattt
    29401 aacggcggca ctggatagac ccaatatcac ggtttatgga ccaaccgatc cgggattaat
    29461 tggcgggtat gggaagaatc agatggtttg tagggctccg ctaatgaatt ta
//
LOCUS       DAPGWD010000046        28520 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPGWD010000046 DAPGWD010000000
VERSION     DAPGWD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.046"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..596)
                     /locus_tag="RHX88_001954"
     CDS             complement(<1..596)
                     /locus_tag="RHX88_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDV2230645.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="RHX88_001955"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="RHX88_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDV2230646.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="RHX88_001956"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="RHX88_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDV2230647.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="RHX88_001957"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="RHX88_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDV2230648.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="RHX88_001958"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="RHX88_001958"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDV2230649.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="RHX88_001959"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="RHX88_001959"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDV2230650.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLTVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="RHX88_001960"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="RHX88_001960"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDV2230651.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /locus_tag="RHX88_001961"
     CDS             complement(4985..5707)
                     /locus_tag="RHX88_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2230652.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNVDRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="RHX88_001962"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="RHX88_001962"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV2230653.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /locus_tag="RHX88_001963"
     CDS             6907..7125
                     /locus_tag="RHX88_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDV2230654.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="RHX88_001964"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="RHX88_001964"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV2230655.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /locus_tag="RHX88_001965"
     CDS             complement(7859..8059)
                     /locus_tag="RHX88_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDV2230656.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="RHX88_001966"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="RHX88_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDV2230657.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="RHX88_001967"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="RHX88_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDV2230658.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..12469
                     /locus_tag="RHX88_001968"
     CDS             10556..12469
                     /locus_tag="RHX88_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2230659.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12469..13491
                     /locus_tag="RHX88_001969"
     CDS             12469..13491
                     /locus_tag="RHX88_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV2230660.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13485..13703
                     /locus_tag="RHX88_001970"
     CDS             13485..13703
                     /locus_tag="RHX88_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDV2230661.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13757..14626
                     /locus_tag="RHX88_001971"
     CDS             13757..14626
                     /locus_tag="RHX88_001971"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDV2230662.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14681..15085)
                     /locus_tag="RHX88_001972"
     CDS             complement(14681..15085)
                     /locus_tag="RHX88_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDV2230663.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15387..16019
                     /gene="crp"
                     /locus_tag="RHX88_001973"
     CDS             15387..16019
                     /gene="crp"
                     /locus_tag="RHX88_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDV2230664.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16070..18160
                     /locus_tag="RHX88_001974"
     CDS             16070..18160
                     /locus_tag="RHX88_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV2230665.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19447)
                     /gene="argD/dapC"
                     /locus_tag="RHX88_001975"
     CDS             complement(18227..19447)
                     /gene="argD/dapC"
                     /locus_tag="RHX88_001975"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDV2230666.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="RHX88_001976"
     CDS             complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="RHX88_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDV2230667.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20128..20730)
                     /locus_tag="RHX88_001977"
     CDS             complement(20128..20730)
                     /locus_tag="RHX88_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDV2230668.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20720..20887)
                     /locus_tag="RHX88_001978"
     CDS             complement(20720..20887)
                     /locus_tag="RHX88_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDV2230669.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="RHX88_001979"
                     /gene_synonym="rotA"
     CDS             complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="RHX88_001979"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDV2230670.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21835..23016
                     /gene="tsgA"
                     /locus_tag="RHX88_001980"
     CDS             21835..23016
                     /gene="tsgA"
                     /locus_tag="RHX88_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDV2230671.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23278..25821
                     /gene="nirB"
                     /locus_tag="RHX88_001981"
     CDS             23278..25821
                     /gene="nirB"
                     /locus_tag="RHX88_001981"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDV2230672.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25818..26144
                     /gene="nirD"
                     /locus_tag="RHX88_001982"
     CDS             25818..26144
                     /gene="nirD"
                     /locus_tag="RHX88_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDV2230673.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26270..27076
                     /gene="nirC"
                     /locus_tag="RHX88_001983"
     CDS             26270..27076
                     /gene="nirC"
                     /locus_tag="RHX88_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDV2230674.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27095..28468
                     /gene="cobA"
                     /locus_tag="RHX88_001984"
     CDS             27095..28468
                     /gene="cobA"
                     /locus_tag="RHX88_001984"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV2230675.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6884 a         7667 c         7226 g         6743 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat gatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga gaacggctcg tcatcacttg cgtgacgttc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatgttac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggtcagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcta ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tggaaatcac cgcttttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcgtct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcgac attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacgtcca acagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agagagcggt
     6121 tcaccacgag tgtaagagtt gtcgaactct ttaccgtcga tcagagtacc tttgtagttc
     6181 acgacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt cttttagaga gttttccatg taacgaccca gcgaggcacc cagtgcataa
     6541 gctgacttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccgcatt attgaggcga aaaaaaagcg
     7141 gggaatcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg gtgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggaccct ggtcagtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcaataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcggcatgca ttgggatgag ctctcgcagc atccgcatat
     8221 ccaggcggcg aaaatgcagt tgcgctcgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacaa cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgagattga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa gcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaacgtac cgagcgccat cgacagcccc aacgcgtcca
     9181 taaacaatgc ggaacccaac accaacagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaaacgg aataccggac gcagtaaata gcgcccgcca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9361 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagaa
     9421 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9481 acgccattgc agttgaagac atcgcaaggc caatgccacc gacaaccgcc gcctgccagg
     9541 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9601 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagttt ggagggattc aactcaagac
     9661 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc catggaccaa ttgcaattcc tgccagcaaa tatcccaaca
     9781 cagcgccaat acccagccgc gatgccagcg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg actcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacatcgc tcatcgggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtaa tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac gacttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcatacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc gccttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggtt gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgttgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt atgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctga ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaacggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aagctcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11821 taaagtaacc gaagaaacgc gccgcttctc aggtggggaa aaagcccgcc tggtgctggc
    11881 attaattgtc tggcagcgtc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11941 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag ttcgaaggcg cgctggttgt
    12001 cgtctcgcac gaccgtcatt tgctgcgttc caccactgac gacctctacc tggttcacga
    12061 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12121 acaaaagcag gaaaaccaga ccgacgacgc gccaaaagag aatgcgaaca gcgcccaggc
    12181 acgtaaagat cagaagcgtc gggaagcgga gctgcgtgcg caaacccagc cgctgcgtaa
    12241 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12301 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12361 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg cgtggctgga
    12421 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12481 acgacgaccg atgccaatga atttagcagc agtgctgaat tcacccctat gcgcggcttt
    12541 agcaattgtc atctacaaac tatgctgccg cgtttgtttc gtcgtcaggt gaaattcacc
    12601 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatctcgc gtggagtgaa
    12661 gaccccgcac aggcgaaaca taaaccgcgt ttagtggtgt ttcacgggct ggaaggcagt
    12721 ctcaatagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12781 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12841 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat
    12901 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12961 aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg
    13021 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    13081 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac gctgccgatt
    13141 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    13201 agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13261 ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13321 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13381 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13441 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13501 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13561 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13621 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatattatgc
    13681 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13741 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13801 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13861 tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13921 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aggctaacga
    13981 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    14041 caaatatctg catacctacg acgaagccgt accgtggaat caggtaccgg ggacattcac
    14101 cccctggcaa cctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14161 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    14221 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14281 acgagaagca gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac
    14341 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14401 cccgttcgcg gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg
    14461 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14521 ccacatcaat acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat
    14581 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14641 cggataaaat actatccggc ttcacaacgg gatagttaaa tcacgcggca accacttcat
    14701 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcag
    14761 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14821 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14881 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatagaaacc acatcgatgg
    14941 cactgcaacc ccccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    15001 agttgccgtc cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt
    15061 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15121 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15181 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15241 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15301 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15361 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15421 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15481 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15541 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15601 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15661 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15721 acattctgat gcgtctgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15781 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaacctgg
    15841 caaaacaacc agatgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15901 aaatcggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg
    15961 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttagt
    16021 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16081 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    16141 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ctctcgttcc
    16201 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16261 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga
    16321 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagtt
    16381 ggggccattg cacgcaaaat tgcttcccgc atcgctgctg gcagccattt ttaccctcag
    16441 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
    16501 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16561 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16621 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16681 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16741 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16801 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16861 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16921 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16981 cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
    17041 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcccgt gacttactgg ccgataaaca
    17101 gcggcgaatg ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    17161 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    17221 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta
    17281 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17341 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat
    17401 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17461 tactattacc gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct
    17521 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
    17581 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17641 tcaggaagcg attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca
    17701 gcgaatgcgg gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca
    17761 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17821 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17881 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17941 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    18001 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18061 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18121 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18181 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca
    18241 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18301 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagccc
    18361 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgaa ctgtggctta agctccgcgc
    18421 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18481 gcaaatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18541 tgatgatatc aaacgccgcg cccgctactg cacaggacag aggattaccg ccgtaggtgg
    18601 aaccgtgaga accaggatga aatgcagaag caatttctgc cgtggtcagc atggcgctaa
    18661 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18721 gcatgtaagc aaacaaatcg ccggtccgcc ccatcccgca ctgtacttca tcaaacacca
    18781 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgctg
    18841 ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18901 tcaccgcttt cactgcgtgg agatcgttaa aaggaacgtg gatgatgtct gccggttttg
    18961 gcccaaagcc gtcggaatat tttggctgcc cgcccaccga gacggtaaac agcgagcgac
    19021 catgaaaagc gttatggaag gcaataattt tggttttgaa cggactatga cgcacacagg
    19081 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19141 ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc gccggttcat
    19201 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19261 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19321 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaatg aactctgccg
    19381 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19441 ttgccatctc atgatcaccc tgttacgcat aaataattgt gaaattataa tcacaaaata
    19501 tgcataaaaa atcactaaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19561 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19621 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcac
    19681 gctggtaatg agtcaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt
    19741 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg
    19801 acctttgcgg cgcgcacaac tttaccgcca aatgcctgcg ccattgcctg atgaccgagg
    19861 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19921 gcttcatctg gcgtacaggg gccaggtgag atgacaattt tttgtggttt aagggcgtcg
    19981 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    20041 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    20101 gctccggtac aaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20161 gctaaatatc atctgcagtg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    20221 ccaggcctct ttttcgatac cctgccagct cagttgatat ccggcatgaa tcgccagttg
    20281 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20341 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
    20401 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20461 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccccg cccagtcgaa
    20521 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20581 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20641 ctgctgctgg tggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
    20701 atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca aggcgcgcta
    20761 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20821 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20881 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20941 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgaaaaatc attacggcag
    21001 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21061 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    21121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21181 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21241 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21481 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21541 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21601 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21661 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21721 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21781 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21841 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21901 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21961 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    22021 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    22081 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg
    22141 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca
    22201 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22261 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22321 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
    22381 tgtgagttcc cggcgctggg caaacatgcg ccaaaaacgg atgctccggt agagaaagaa
    22441 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22501 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
    22561 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22621 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22681 attctgatgt acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct
    22741 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22801 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
    22861 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22921 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22981 cgtcaccgtc agcataacac cctgacctct cattaattgc taatgccgga cggcactatc
    23041 gtcgtccggc cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgttca
    23101 ttttgtctat tttttgcaca aacatgaaat atcagacaat tccgtgactt aagaaaattt
    23161 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    23221 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23281 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23341 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23401 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23461 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23521 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccggacg taccgttttt
    23581 tatgacaagc tgatcatggc aaccggttcc tacccgtgga tcccgccaat caaaggttct
    23641 gatactcagg actgctttgt ctatcgcacc attgaagacc tcaacgccat tgaatcctgc
    23701 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23761 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23821 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23881 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt
    23941 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    24001 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    24061 gggattgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    24121 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac caggctacaa aatggcgcag
    24181 gtcgccgttg accatattct gggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24241 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24301 ggcgcacgta gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc
    24361 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggt
    24421 aacctgctgc aactggtgct gaacgctatc gaactgccgg aaaacccgga ttccctgatc
    24481 ctgccagcac actctggtag cggcaagccg tctatcggtg ttgataaact gccggacagc
    24541 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24601 ggctgccaca ccgttgcggc gctgaaagcc gaaaccaaag cgggtactgg ctgcggtggc
    24661 tgtatcccgc tggtcactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
    24721 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
    24781 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ttacggttgt
    24841 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24901 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24961 gacggcacct actcggtgat cccgcgctct ccgggcggtg aaatcacccc ggaagggctg
    25021 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaaaatcac cggctcccag
    25081 cgcctggcga tgtttggcgc acagaaagac gatctgccgg aaatctggcg tcagctgatt
    25141 gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    25201 gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg
    25261 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25321 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25381 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
    25441 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25501 cgtaccgccg acaaactgac gcgtaccgca ccgtggttag aaaaccttga aggcggcatt
    25561 gattacctga aagcagtgat cattgacgac aaactggggc tgaacgctca tctggaagaa
    25621 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacgccg
    25681 tctgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
    25741 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
    25801 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25861 ctgaaaccgg cgtctgcgcg ctgttaggtg acgagcaggt agcaattttc cgcccgtatc
    25921 atagcgacca ggtgtttgcg atcagcaaca tcgacccgtt cttcgagtcc agcgtgctgt
    25981 cacgcgggct gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    26041 agcgttttcg cttaagtgat ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    26101 acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    26161 gcaatgcctc ccctttttgc atggtcctgt aatgaccttc ggtatattgc aggacatttt
    26221 ttaaactttt tgttttattt tttgttttta ttttttaaag gataatcaaa tgtttacaga
    26281 cactattaat aagtgtgcgg ctaatgctgc gcgcattgca cgcctgtcgg caaataaccc
    26341 gctcggcttt tgggtcagct ccgccatggc gggcgcatat gtgggtcttg ggatcatcct
    26401 gattttcacg ctcggtaatt tgctcgaccc gtccgtacgt ccgctggtga tgggcgcgac
    26461 ctttggtatc gccttaacgc tggtgattat cgccggttct gaactgttca ccggacacac
    26521 catgttcctc acctttgggg taaaagcggg cagcatcagc cacgggcaaa tgtgggcaat
    26581 cctgccgcaa acctggctgg gtaacctggt cggttccgtc ttcgttgcca tgctctatag
    26641 ctggggcggc ggtagcctgc tgccggtaga taccagcatc gttcactccg tcgcgctggc
    26701 taaaaccact gcaccggcaa tggtactgtt cttcaaaggt gcattgtgta actggctggt
    26761 ttgcctggca atctggatgg cgctgcgcac tgaaggggcg gcgaaattta tcgctatctg
    26821 gtggtgtctg ctggcattta tcgcgtctgg ctacgagcac tctatcgcta acatgacgct
    26881 gttcgcgctc tcctggttcg gcaaccacag cgaagcctac acgctggcgg gtattggtca
    26941 taacctgctg tgggtgacgc tgggtaatac tttatcaggt gccgtattca tgggattggg
    27001 ttattggtat gctacgccga aagcgaatcg tccggttgcg gacaaattta atcaaactga
    27061 aacggctgcc ggttaattac taaggggttt ttacgtggat catttgccta tattttgcca
    27121 attacgcgat cgcgactgtc tgattgtcgg cggtggtgat gtcgcggaac gcaaagcaag
    27181 gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca ttagcgttta ttccacagtt
    27241 caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa gggccatttg atgaaagcct
    27301 tctcgacacc tgctggctgg cgattgcagc gacggatgat gacgcgctta accagcgcgt
    27361 cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgtg gtcgatgcgc cgaaagccgc
    27421 cagctttatt atgccgtcga ttattgaccg ctcaccgctc atggtcgcgg tctcctctgg
    27481 cggcacctct ccggttctgg cgcgcctgtt gcgcgaaaaa ctcgaatcac tgctgccgtt
    27541 gcatctgggc caggtggcga aatatgccgg gcaattacgc gggcgagtga aacaacagtt
    27601 cgccacgatg agtgagcgtc gccgtttctg ggagaaattg ttcgttaacg accgcctggc
    27661 gcagtcgctg gcaaacaacg atcagaaagc cattactgaa acgaccgaac agttaatcaa
    27721 cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt gcaggtccgg gcgatgccgg
    27781 gctgctgacg ctgaaaggac tgcaacaaat tcagcaggca gatgtggtgg tctacgaccg
    27841 tctggtttct gacgatatta tgaatctggt acgccgcgat gctgatcgcg ttttcgtcgg
    27901 caaacgcgcg ggataccact gtgtaccgca ggaagagatt aaccagatcc tgctgcggga
    27961 agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc gatccgttta tttttggccg
    28021 tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt ccgttctcgg tggttccggg
    28081 tattaccgca gcttctggtt gctctgccta ttcgggtatt ccgctcacgc atcgcgatta
    28141 tgcccagagc gtgcgcttaa ttaccggaca cttaaaaacc ggtggcgagc tggactggga
    28201 aaacctggcg gcagaaaaac agacgctggt gttctatatg gggctgaatc aggctgcgac
    28261 tattcagcaa aagctgattg aacacggtat gcctggcgaa atgccggtgg caattgtcga
    28321 aaacggaaca gcagtcacgc agcgcgtgat tgacggtacg ctcacgcaac tcggtgaact
    28381 tgctcagcaa atgaacagtc catcgctaat tattattggt cgggttgttg gcctgcgcga
    28441 taaactgaac tggttctcca accattaatt taacccgggc cagagaattc tggccttctt
    28501 aatgactctt tttatatatt
//
LOCUS       DAPGWD010000047        28161 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPGWD010000047 DAPGWD010000000
VERSION     DAPGWD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.047"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..884
                     /locus_tag="RHX88_001985"
     CDS             <1..884
                     /locus_tag="RHX88_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDV2230676.1"
                     /translation="ENFNEQLDTNVNIIISILNRRDIVLESLQPYLVINKDAVTPCTF
                     LGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLN
                     EDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPITVSSDEMTNALDPVEL
                     AAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVV
                     AHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEK
                     NRNGLMPLIRRRTMLQL"
     gene            1250..2317
                     /locus_tag="RHX88_001986"
     CDS             1250..2317
                     /locus_tag="RHX88_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2230677.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2314..5049
                     /gene="rbbA"
                     /locus_tag="RHX88_001987"
     CDS             2314..5049
                     /gene="rbbA"
                     /locus_tag="RHX88_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDV2230678.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5049..6173
                     /locus_tag="RHX88_001988"
     CDS             5049..6173
                     /locus_tag="RHX88_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2230679.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6246..6521
                     /locus_tag="RHX88_001989"
     CDS             6246..6521
                     /locus_tag="RHX88_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDV2230680.1"
                     /translation="MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6518..6877
                     /locus_tag="RHX88_001990"
     CDS             6518..6877
                     /locus_tag="RHX88_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDV2230681.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6997..7398)
                     /gene="nikR"
                     /locus_tag="RHX88_001991"
     CDS             complement(6997..7398)
                     /gene="nikR"
                     /locus_tag="RHX88_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDV2230682.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7404..8210)
                     /gene="nikE"
                     /locus_tag="RHX88_001992"
     CDS             complement(7404..8210)
                     /gene="nikE"
                     /locus_tag="RHX88_001992"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDV2230683.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8207..8971)
                     /gene="nikD"
                     /locus_tag="RHX88_001993"
     CDS             complement(8207..8971)
                     /gene="nikD"
                     /locus_tag="RHX88_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDV2230684.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8971..9804)
                     /gene="nikC"
                     /locus_tag="RHX88_001994"
     CDS             complement(8971..9804)
                     /gene="nikC"
                     /locus_tag="RHX88_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDV2230685.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9801..10745)
                     /gene="nikB"
                     /locus_tag="RHX88_001995"
     CDS             complement(9801..10745)
                     /gene="nikB"
                     /locus_tag="RHX88_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDV2230686.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10745..12319)
                     /gene="nikA"
                     /locus_tag="RHX88_001996"
     CDS             complement(10745..12319)
                     /gene="nikA"
                     /locus_tag="RHX88_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDV2230687.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12430..13017)
                     /gene="acpT"
                     /locus_tag="RHX88_001997"
     CDS             complement(12430..13017)
                     /gene="acpT"
                     /locus_tag="RHX88_001997"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDV2230688.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKSAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13072..14121)
                     /locus_tag="RHX88_001998"
     CDS             complement(13072..14121)
                     /locus_tag="RHX88_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV2230689.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14253..15470
                     /locus_tag="RHX88_001999"
     CDS             14253..15470
                     /locus_tag="RHX88_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2230690.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15474..16031)
                     /gene="dcrB"
                     /locus_tag="RHX88_002000"
     CDS             complement(15474..16031)
                     /gene="dcrB"
                     /locus_tag="RHX88_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDV2230691.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16104..16769)
                     /locus_tag="RHX88_002001"
     CDS             complement(16104..16769)
                     /locus_tag="RHX88_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDV2230692.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16990..17235
                     /gene="tusA"
                     /locus_tag="RHX88_002002"
     CDS             16990..17235
                     /gene="tusA"
                     /locus_tag="RHX88_002002"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDV2230693.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17337..19535)
                     /gene="zntA"
                     /locus_tag="RHX88_002003"
     CDS             complement(17337..19535)
                     /gene="zntA"
                     /locus_tag="RHX88_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDV2230694.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTLVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALTIPAAESQRALVGSGIEA
                     QVNGEHVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19609..20235)
                     /locus_tag="RHX88_002004"
     CDS             complement(19609..20235)
                     /locus_tag="RHX88_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDV2230695.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20376..20735
                     /locus_tag="RHX88_002005"
     CDS             20376..20735
                     /locus_tag="RHX88_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDV2230696.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20738..21007)
                     /locus_tag="RHX88_002006"
     CDS             complement(20738..21007)
                     /locus_tag="RHX88_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDV2230697.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20997..21593)
                     /gene="rsmD"
                     /locus_tag="RHX88_002007"
     CDS             complement(20997..21593)
                     /gene="rsmD"
                     /locus_tag="RHX88_002007"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDV2230698.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21743..23239
                     /gene="ftsY"
                     /locus_tag="RHX88_002008"
     CDS             21743..23239
                     /gene="ftsY"
                     /locus_tag="RHX88_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDV2230699.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23242..23910
                     /gene="ftsE"
                     /locus_tag="RHX88_002009"
     CDS             23242..23910
                     /gene="ftsE"
                     /locus_tag="RHX88_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDV2230700.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23903..24961
                     /gene="ftsX"
                     /locus_tag="RHX88_002010"
     CDS             23903..24961
                     /gene="ftsX"
                     /locus_tag="RHX88_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDV2230701.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25206..26060
                     /gene="rpoH"
                     /locus_tag="RHX88_002011"
     CDS             25206..26060
                     /gene="rpoH"
                     /locus_tag="RHX88_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDV2230702.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26331..27434
                     /gene="livJ"
                     /locus_tag="RHX88_002012"
     CDS             26331..27434
                     /gene="livJ"
                     /locus_tag="RHX88_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDV2230703.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKDNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27633..28016)
                     /gene="panM"
                     /locus_tag="RHX88_002013"
     CDS             complement(27633..28016)
                     /gene="panM"
                     /locus_tag="RHX88_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDV2230704.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6699 a         7298 c         7805 g         6359 t
ORIGIN      
        1 gggagaactt taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca
       61 gaagagacat tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca
      121 cgccatgtac gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata
      181 aatttatcat cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag
      241 ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac
      301 cacttaatga ggatacctac gctgggataa aagattatcc tgtatgtttc tatggcccca
      361 aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca
      421 ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat
      481 tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata
      541 gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac
      601 ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac
      661 ataacggtaa tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg
      721 cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg
      781 gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg
      841 gacttatgcc tttgattaga aggcgcacaa tgttacagct ctgaaccata gacaacatga
      901 atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc
      961 ctgcaactga acaggcgtga aacgcgatcc aacaggatgg ataccgtttt gtcagccagt
     1021 tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga
     1081 caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg
     1141 gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg
     1201 gcagttgtaa cacgcgtcgt ttgcttacag ggattatggc gggccgatta tggataagag
     1261 taagcgccat ctggcgtggt gggttgtcgg ggcactggcg gtggctgccg tcgtggcgtg
     1321 gtggctgttg cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga
     1381 agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa
     1441 agaaggccag tttgttcgcg aaggtgaagt gctggcgaag atggatactc gcgtgttgca
     1501 ggaacagcga ctggaagcca tcgcgcaaat caaagaggca caaagcgccg ttgccgccgc
     1561 gcaggctttg ctggagcaac gacaaagcga aactcgcgcc gcgcagtcgc tggttaatca
     1621 acgccaggca gaactggact ccgtagcaaa acgtcatatg cgttcccgtt cactggccca
     1681 acgaggggct atttctgcgc aacagctgga tgacgaccgc gccgccgctg agagcgcccg
     1741 agctgcgctg gaatcggcaa aagctcaggt ttctgcttct aaagccgcta tagaggcggc
     1801 acgtaccaat atcattcagg cgcaaacccg cgtcgaagcg gcacaagcca ctgaacggcg
     1861 cattgccgca gatatcgatg acagcgaact gaaagccccg cgtgacggac gcgtgcagta
     1921 tcgggttgcc gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga
     1981 tctcagcgac gtctatatga ccttcttcct gccaaccgaa caggcgggca cgctgaaact
     2041 gggtggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat
     2101 cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg
     2161 gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct
     2221 ggaatatgtc aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaacttcc
     2281 gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc
     2341 ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaccgt tgcgctgaac
     2401 aatattactc tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc
     2461 gggaagtcga gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg
     2521 atggtgctgg gcggcgatat gcgcgacccg aagcatcgcc gcgacgtctg cccgcgcatc
     2581 gcctggatgc cgcaggggct gggcaaaaat ctctaccaca ccttgtcggt gtatgaaaac
     2641 gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat
     2701 gagctgctga ccagcaccgg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc
     2761 ggcgggatga agcaaaaact ggggctgtgc tgtgcgttaa tccacgaccc ggaactgttg
     2821 atccttgatg agccaacaac gggggttgac ccgctctccc gcgcccagtt ctgggatctg
     2881 atcgacagta ttcgtcagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg
     2941 gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact
     3001 ggcagcgccg aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata
     3061 aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa
     3121 cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc
     3181 gttgccgttg atcacgtcaa tttccgcatt ccacgcgggg agatttttgg attccttggt
     3241 tcgaacggct gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc
     3301 gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt
     3361 cgggtgggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac
     3421 cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct
     3481 gaaatgagcg agcgttttaa gctcaacgac gttgaagata ttctgccgga gtcattgccg
     3541 ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta
     3601 atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg
     3661 atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctccaccca ctttatgaac
     3721 gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt
     3781 acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc
     3841 tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac
     3901 accacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc
     3961 gaagcgctgg aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg
     4021 atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg
     4081 gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc
     4141 cgttacttta tcgaacagcc gccgctcacc agttatgacg agcttgatcg tcggatgcgt
     4201 gcgggcgata tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt
     4261 ggtacgcctg tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg
     4321 gtaaaaggtt acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa
     4381 tcgacacccg ccagccaaag cgggctgatg aacattgaga cgcgttatcg ctataacccg
     4441 gacgtaaaaa gcctgccagc gattgttcca gcggtgatcc cgcttctgct gatgatgatc
     4501 ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agcttgggtc gattatcaac
     4561 ctttacgtga cccccaccac ccgtagcgag tttttgcttg gcaaacagct gccgtacatc
     4621 gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta
     4681 ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcatcgcc
     4741 accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga
     4801 acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct
     4861 tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact
     4921 atcgcccgcg ggacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc
     4981 ccgttgctga tagccatccc gctggtgatg ggcttaagta tcctgctgct gaaaaaacag
     5041 gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc
     5101 tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt
     5161 cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg
     5221 atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac
     5281 cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg
     5341 cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc
     5401 aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga
     5461 atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt
     5521 tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg
     5581 gggtgatggc gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc
     5641 tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt
     5701 ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat
     5761 tatcgctggt gctgatggtg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc
     5821 tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg
     5881 ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc
     5941 tgcaaatgct ttccggtggt tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca
     6001 ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg
     6061 gggcaggatt cgaaatcgtc tggccgcagt tcctgacact gatggcaatt ggcggcgcgt
     6121 ttttcaccat tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg
     6181 aaacttgact tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac
     6241 atgccatgag aaccaaggta caggctttac ggaagaaaca aaaaaataca ttggatcaaa
     6301 tagttaaaac tcctgttcct caagggatca agtggtctga tatagagtca ctggttaaag
     6361 cattaggcgg agaaattaag gaaggaagag gttcgcgttg caagttcata ctaaatatga
     6421 gcgttgcgtg tttccatcgg cctcatccgt cgccagatac cgataaaggc gctgtagaaa
     6481 gcgtgcgtga ctggttacta agtatagggg taaaaccatg atcaaactga agacgccaaa
     6541 ctctatggag attgccgggc aacctgcagt tatcacttac gtgccagaac tgaatgcatt
     6601 tcgtggcaaa ttcctgggct tgtcaggtta ttgtgatttt gtctctgaca gcattcaggg
     6661 gctgcaaaaa gaaggggaac tttccctgcg agagtatctt gaggattgta aagctgcggg
     6721 tattgagccg tatgcgcgta cagaaaagat taagaccttt acgttacgct atccagaatc
     6781 gctgagtgaa cgccttaata atgcggctgc gcagcaacaa gtctctgtta atacctatat
     6841 tattgaaaca ctgaatgagc gtttgaatca tttataaatt ttagcggcgt acattacgca
     6901 gaatatctgc ctttacagga tctatctatg caactgaact taattttata aattaagttc
     6961 agttaatatc ttttctttcc tcgtttcaga aataactcaa tcttccttcg gcaagcactg
     7021 caaatgcccg tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc
     7081 gcccatatca cctttcagca cggcgatttc cagacagtcg tcgtgattga tatgcacatg
     7141 cagcgtggcg acggagagat cgtggtgatg gtgctgggtg gagacaatgc ggctggctaa
     7201 gtcgcgtttt tcgtgttcat acacatagga gagcaccgcg aaaccttgcg tgccgtgctg
     7261 ctgagtggcc tcctgggcca gagcgctacg cagaatatcg cggatagctt cggaacgatt
     7321 gttataacca cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt
     7381 gatggtgact cgttgcattt gcgttaaacc ttttctgtgg tgcgacggcg cacggggaat
     7441 gcgggtaata ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt taatttatct
     7501 cccaccacct gggtttcgac gatttgtccg ttgtccatca ccatcacccg ctgacagaag
     7561 cgttccacca ggcgtaagtc gtgggtgatg aacaggcagg cggtgccaaa ctgttgttgt
     7621 agctttttca gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt agaaacggct
     7681 tcatccagaa tcagtagttt cggttcgacc gccagcgcgc gagccaggca gacgcgctgg
     7741 agctggccgc cgcttaactg cggtgggcgt ttgtcgagaa cgctgtcatc gagatcgacc
     7801 gccttcagca tttcgctggc gcgcgccagt tgttcggatt ttttcagtga tagcaggtgg
     7861 cgcatcggtt cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc gctgatggag
     7921 tcctgaaata ccatctgaat atcgcggcgg aacgctttac gctgggcgcg attgagtttc
     7981 gccagcggtt cgccgcgcca gctaatattc ccttgtgagg gagactctaa gcctaccagc
     8041 agccgcgcga gggtgctttt tccgcagccg ctgcgtccca gcagggcgac ggtttcgccg
     8101 cttttcaggg tcagggaaac gttattcagc accgcctgat gttgatgttt tccgctaaat
     8161 ccaccgtggg catagtgatg ggaaaggccg cagacgttaa gtaaggtcat gatgccagct
     8221 ccataccgta gagggcgaga tgagcggaaa ccaggctgcg cgtcaccgta tgtttggggg
     8281 cgttaaacag cgtttctaca tcgccctgtt cgacaatttt accttgtgac atcaccgcca
     8341 cgtcatccgc cagacgcgcc acaacgccca tatcatgggt gaccagcagc attcccggcg
     8401 cttgtttttg cataatgctt tccagcagat cgaggatgcg cgcctgtgct accacgtcga
     8461 ggtcggtggt cggttcatcg gcgatgataa acggtgattc acacagcacc gccatcgcaa
     8521 tcatcatgcg ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc agcacgcgcg
     8581 cggcgttttc cagccccacc gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt
     8641 tccctaacgc caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgcagt ggattaaagg
     8701 cgctgcgcgg gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg caaggcgaaa
     8761 ccggtttgcc atcggctaaa atttcccccg ccgtctggcg aacgccagcg ggcaaaatgc
     8821 ccagcgtcgc ggcgcaggtt aatgatttcc cgctgccgct accgccgact aacgccagca
     8881 cgcgcccgcg ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg gcctgtagcg
     8941 cgatattacg tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg
     9001 atccagatga tcgcgcagtg cgtcacccac caggttaaag gccatcacgc tgataaacag
     9061 cgccagcccc ggccagaaca tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt
     9121 aatcatcacg ccccattcgg cggtcggcgc ggtcacaccg aggccaagga aagacatccc
     9181 cgcgacgtgc agcatcatat ggccgatatc cagcgttgcc agcaccagca gcgaagggat
     9241 caccgcgcct gccagatgat cgacaaacac ccgcacatgg cccgcaccgg aaagccgtga
     9301 cgccagcaca aactcgcgtt ggcgtagtga aatcaccagg ctgcgcacca tgcgtgcata
     9361 ccacgcccag tgcgacaggg cgatggcgat aattacgttg gtcagcccgg tgccgagcac
     9421 gccaaccata aagaacgaca gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg
     9481 catggtggcc tgatcaacgc gtccgccaat cagtccggcg ctgccgccaa tcaccagccc
     9541 taatgtcagc accagcagca ggcaggccat taccgaaccg agcgacacgc gggtcgccgc
     9601 catcagccgc gagaaaatat cacgccctaa gtgatcggtg cccagccagt gctgcgcatc
     9661 cggcgaaagc aggcgcgacg gcagatcaat agcctgaggg tcatacggca gccaccactg
     9721 gctggtaagc gcaatcagcg ccagcagggc gataatgatc agcgccaggc gtaccgacca
     9781 gcgggaagag aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat gcgcgggtcc
     9841 agcgcggcgt tgagcaaatc gacaatcaaa ttacagacca caaaaaccac caccatcatc
     9901 agcgtaaagc actggatcac cggatagtca cggttaaaaa tcgccgacac cgcatagcgc
     9961 ccgacgcccg gccaggcaaa gatgttttcg ataatcatcg tcccgccgat cagttcgccg
    10021 atgtgcatcc ccacggcggt gatcatcggc agcgaggcat tgcgcaggat gtggcgacgt
    10081 tcggtctgtt tgtcgttcag gccgcgcaga cgcgcccagg tgacgtgacg ctgaccggcg
    10141 acgtccagca tactggcgcg cagtaaacgc gcgttaatcg ccagcgacat aaaggcaatg
    10201 gaaaccgcag gcaaaatgat gtgctgccag ccgccgtaac ccatcgcggg tagccattgc
    10261 agatacaccg aaaacgccat caccagtaaa aacgccagcc agaagttagg catcgacacg
    10321 ccaagaaacg cgatgaaacg tacggcgaaa tccggcagac ggtcgcggtg gcgcgccgcc
    10381 cagataccga gcggtacgga agtgagcaga attaatacca gcgccgcacc tgcaagttcc
    10441 agcgtggcgg gcaggaagtt cagcatatcg tccagtaccg ggcgttggct ggcgaatgag
    10501 ataccaaagt caagatgcag cgccttccac aaccaggtgc cgtactggac gtacagcggc
    10561 tgatccagtc ccagcatggt gcgggtagag gccagcatct ccggcgtcgg cggcaggtta
    10621 gacagacgca aataatcgag cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg
    10681 atcaccgagg cggcgagcac catcgggatc agcagcagaa agcggcgtaa tacgtaacgc
    10741 aacattaagg tttcaccggt ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt
    10801 aggggatgtt acccagctcc ggttttgata ccaccatcat cgagatgtag ctgataggca
    10861 gataaaccgc ctcgtcatgc agacgggtca gaatgtcgcg atacagcgcc tgacgttgcg
    10921 tttcgtcatg ggtcgccagc acttcgccga tctctttatc aatcagcggc ttgtcggcta
    10981 atccttgctg tgcctggaag tcagcgtgcg acggtacgcg cattgaactc aggaaggcgt
    11041 gtggatcgta tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct
    11101 gacgagcata gatactgctc tcttcttcgc caatcagcga gacatctgcg ccaatctggc
    11161 gcatatcagc ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg
    11221 acagttcaat gcgcagcggc tgaccatttt tctcgcggat gtctttgccc gcaggcagcg
    11281 tccaaccggc tttttccagc aactctttcg ctttctgcgg atcgtactgg cgcggtttca
    11341 ggccgagatt agcgtaaggc acagaagggg caaacagggt gtcggcgacc tgctgggtgc
    11401 catataacgc gttatcaatc agcgattttt tgtttaccgc gtaattaaga gcttcacgta
    11461 ctgccagctc gttggtgggg gctttggcgg tattgagcgc cagcatcacg gtttcgatcg
    11521 gctgtgacag ttgggtgtgg taagccggat tctggctaaa gcgggcgaag gtatcgagcg
    11581 gtaataaccc ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc
    11641 gggtagtcgg gtccgggatg acgttaaagg tgatcttttt aatcgctggc ttttcgcccc
    11701 agtagttttc gttacggacg aagacatcgt actgattcag tttcgattcc tgcaaaatcc
    11761 acggtccggt gccaatcggc gctttaattc cgttcatggt ttcatggttt ttaaactgcg
    11821 agggagcgat aaaacggaaa ggacggggca gggccagttc ttgcaggaaa ggatagtagg
    11881 cgcttttcag ggtaatttgc agctctgttt tactgagtgc tttaacatca acaatctggt
    11941 ttgccagctc cagccaggcg tgacgttgac ggttatcgag cactgcgcgg aagttttctg
    12001 ccgccgcctc ggcatcgaac ggttcaccgt tggagaattt cacgtcatca cgcagggtga
    12061 aggtccaggt tttaccatct tctgaatgag tccagctttt tgccagccac gggatcaccg
    12121 aaccgtctgc ctgatatttc accaatggtt cataaaccat gctctgggcg aacatctggt
    12181 taggcgtgta aaggtgcggg tttagtggcc cgacattcac cggccaggcg gtggtgattt
    12241 catctggtgc agcggcatgg acgataaaag acgcacaagc cagcagcgca aatagagtgc
    12301 ggcggagtgt ggagagcatg gtaaccccaa tggattaaaa tagatggcgg aaataagtat
    12361 gacgatttta agtattcgtc atactgatta cctgttctgg atcaatagat gggcagtcgg
    12421 gtgggcggat cagttaactg aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg
    12481 ggtgggggtg cagatcgcca ggcttaaatt ttcaagctga caatggctga ctgacagcga
    12541 ggagtgatag gtgctgtcta cgctgacgat ttgccaggcg ctgccgccac gctgtttaac
    12601 gatggcttct ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat caggatgcac
    12661 ggcgtccatc tcagcgtgtt ccccgaggct gaatacggcg ttcgccagcc agcgccagtt
    12721 ggcgcgcggg cgaatcactt cgatatcgca gccgacttcg ccttcatcac tcaacagcag
    12781 agcgatatcg tcaccgctat ggcttaagtt gaaccatagc ggcgtttccg gcgcgaatgc
    12841 agatttgcct tgttcgccat agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa
    12901 tgcacgcccc gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc
    12961 cggtggcagt ggagctgcgc ttaaggtcga aactttcccc agaactatcc gatacatatc
    13021 agggccaacg tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg
    13081 taaccgactt ttcggtctgc ccggccccag taaaatcgcc agtttgctgc cacctttcgt
    13141 cgtttccatc cagattttac acacgctggt taatggcacc gaaagtagca tccctaccgg
    13201 gccgagcagc catccccaga ttaacaatga aagaaatacc accatggtgg acatccccag
    13261 gcgatggccc atcatccgtg gctctaaaat attgccgatg accatatgga caactaaaaa
    13321 caatgcgccg accagaatac attcgtaaat accattaaac agcagcacct gaatcattgg
    13381 cggtacggcg gaaattaccg cgccgatatt gggcacgtag ttaagcaaaa acgccagtac
    13441 tgcccacatc agcgcaaact gtacccccat cagctccagc cccagccaga cgatgacacc
    13501 tgtccataaa ctgagtagcg tcttcaatgc aagatagtgc gaaacgcctt taagtgcgcg
    13561 gtgtaatccc gcgatgtgaa tctgtggatt attcagcgca aaacgcattt tgtaagggac
    13621 gtggcgcact tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat
    13681 tgccccggaa agcccggtca ttagcgctgt ggtgaaggta accacttttt ccgagtccat
    13741 ccgctgcagc attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa
    13801 tttaaaaagt ttgcgcgtca gctccttatt aaacttcggc agcatagaga taaattcgtt
    13861 aaacgatgcc gccagtacgc cgaccagcgc ggttagtgcg atcagcatca ccaccactac
    13921 aatcgtaatg gcaacggggc gttgtactcc ccgacgaata aaccaggtaa cgagcgggtt
    13981 gaggacgatg gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg
    14041 aatgcccgcg aggatcacta ccagcgaggc cagcttgagc agaatgtgca tgcccgtttt
    14101 atcgggttga ggggtttcca ttggggcttc cttgtgactt tttgtattaa gtgtagcggg
    14161 agtcacgcca gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa
    14221 tgaaacactg ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc
    14281 taaacggatt gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct
    14341 acctcaccat cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct
    14401 ttagcgcttt ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc
    14461 gtcctcatgc cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt
    14521 tatgcggctg ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc
    14581 ccgtcatcag cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt
    14641 ttgccggaac gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc
    14701 gagtgatttc gtggaacggc attgtcactt acggggcgat ggcgatgggt gcgccgttag
    14761 gcgtcgtgtt ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg
    14821 cgctggtggc cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac
    14881 cgctgccgtt tcgcgcggtg cttgggcgcg tctggctgta cggtatggca ctggcactgg
    14941 cttccgccgg atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaggtt
    15001 gggacggtgc ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt
    15061 tattccctaa cggcattaac cgtatcggcg gcttaaacgt agcgatgatt tgctttagcg
    15121 ttgagataat cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaaatcg
    15181 gcgtcttact ggcgggggca gggttttcgc tggtgttccc ggcattgggt gtagtggcgg
    15241 taaaagcggt tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg
    15301 atttatcgct tggcgtgact ggaccactgg ctgggctggt gatgagctgg gcgggcgtac
    15361 cggtgattta tctggcggcg gcgggactgg tcgcaatcgc gttattactg acgtggcgat
    15421 taaaaaaacg gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga
    15481 ataaccagcg tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagcg
    15541 ggcagcgtaa tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag
    15601 tacgccgtct ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc
    15661 agctcaatgg ctttattggt taccacttgc agctgcggat cgcggctacg ttgctgatct
    15721 tccagacgct tcgccagcac cgccagatct tctttcggat catcgcccat gatgacgatg
    15781 actgctttct gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc
    15841 ttaccgctct ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg
    15901 ttgacgggat tcccggtagc gttactttcc gcgaccgaac cctgcgccgt agcgttagtg
    15961 tctttatcat cacaggccgc aagccccata accagcaggc caattccgac atatttaacc
    16021 agattgcgca ttgacttctt cctttcgata aacggccata acggctcatt catccatctt
    16081 atcacaactc tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct
    16141 gccagtcttt tcaacagcat attgagtaat acgccataca ttggcaggaa gaaaacgata
    16201 ctgattaaca ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata
    16261 aaggcatccg ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta
    16321 ccgaacagtg tggacgctgt cggtgccagc caccagcggc gactctgacg caggcggtta
    16381 aaaacgtgca cgtcgaggat ctgtcccagc gcgtaggcca tgaaactggc ggtggcgata
    16441 cgggcgacaa acaggttgaa gtgggcgagt gcgccgaatc cctgccatga acccatatag
    16501 aacagcgacg agatgacata ggagattaat aacgcaggga tcattaccgc gaagataatg
    16561 cgtcgggcca gcggtgcgcc aaaaatacgc acggtcaggt cggtcgcgag aaagataaac
    16621 ggaaaactaa acgcgcccca ggtggtatga aaaccaaaaa tggagaccgg aagctggacc
    16681 agatagttac tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta
    16741 tagcgttgag tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga
    16801 gggaacccaa tacgtacgac acgtctgtta tcttaacata ataacgtgct tacccttttt
    16861 tcgagccgcc gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc
    16921 gttaacctgg tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttaagtgat
    16981 gagaagaaaa tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg
    17041 cgctgcccgg aaccggtgat gatggtgcgc aaaactgtgc gcaatatgca gcctggcgaa
    17101 acgttgctga ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc
    17161 tttatggaac acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt
    17221 cgtaaaggcg gttgatgggg aaagattggc ttcgatgccg ccttttcccc tcaccctaac
    17281 cctctcccca gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat
    17341 ctcctgcgca acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc
    17401 agcaccgcca gccatagtcc ggtcatccct aaaagcgtgg tgacgaggaa gatccctttc
    17461 agccccagcg caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca
    17521 atcatttgca ccaggccgcg caggtggtta tgggttaatg ctgcatcagc ggtttccagc
    17581 gccacgtctg tgccactgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg
    17641 tcgttaatac catcaccgac catcgccagg ggcgcatgtt gattcagctt ggtcaccgct
    17701 ttgactttat cttccggcaa taggcccgct ttaaactcca gccccagctc cccggcgatt
    17761 gccgccgctg cacgtggatt atcgccggtg aggatcactc ctttgacccc cagcgcgttc
    17821 agttcactga tggcagttgc agcatcggcg cgcagggtat cctgcaatgc aatgataccc
    17881 agcacgtcat cgttacgtac taccagcact accgtttgcc cggcgctttc cagttcatta
    17941 atcaaaccag caaatgcatc agcgggatgt ttccctgcag cgcatatcaa cacgtgctca
    18001 ccgttaacct gcgcttcaat gccagacccg accagcgccc gctgtgactc ggcggcggga
    18061 atggtcagtg cagcaacctg tgcttctcgc acgatggcct gcgccagtgg atgcgtcgcg
    18121 ccttgctcga ccgccgccgc cagtgtcagc agttcagatt cactaatacc cgttgccgga
    18181 tgaatcgcgg taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc
    18241 agggtaacac gacccagttg ttccagcgcc gctccgcctt taattaacgc cccacgacgc
    18301 gctgctgctg ccagcccgga ggtaatcgcc gcgggcgtgg agataactaa cgcgcacggg
    18361 cagccaatca gcagcagcgt caggccttta taaatccact cctgccagct ggcggcaaac
    18421 agcagcggcg gcaccagcgt taccagcagg gcgacggcca taatcgctgg cgtataaata
    18481 cggctgaaac ggtcgataaa ccgctcaatg ggggcgcggc gctcttcggc ttcttcgatc
    18541 agtttcagaa tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc
    18601 agacggtcta cgctggtggc tccagcaggg actttatcgc ccgtcgcgcg ctccaccgga
    18661 atggattctc cggtcagggc gctttcatca aaactggcaa acggtgagag caatttaccg
    18721 tcggcaggca aacgcccacc tgcggcgact tcaatcacat cgccagggcg caggctgtta
    18781 atcgccacct cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc
    18841 attaacgcgc tgaccccttg acgtgcgcgg ctggcggccc agccttccag tcgttcgcca
    18901 atcaaaaaca gcagcaacac catcgcagct tcagccgttg cgccaataaa cagcgcacca
    18961 atagcggcta cgctcattaa cgtttcaatg gcgaagtagc tgccggattt gatcaaccgt
    19021 aatgcctgac gagcaatcgg gtacagccca accagcgtgg tcgcgataaa cgccagttgc
    19081 ccgaacggat gattgaactg ctccaaaccc cagctgattg ccatcatcac gataagcgta
    19141 atcagcggca gattctcttt caggcgcgat gcttgtggct cgtcggcggc ctgctcatcg
    19201 cgcagggaat agcccgcttt ttgcaccgca gattcaacct gggcacggat gtcattgtcg
    19261 gcatcaacca ccagtttttc ggtggcgaac aacacctgta cctgattcac gcctgcaagc
    19321 tggcgcacgg cattttctac cttgcgcgca caggcggcgc agtccatgcc gctgactttc
    19381 cagctatagc gggtgccgga gacgttttca gagagagttg gcgtgctgga acatgcgccg
    19441 tcgcagcaac agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga
    19501 ggggctttct tgccgtgatt gtcaggagtc gacatggcat cctccggtta agttttttct
    19561 cattaaccga aggatacact ctggagtcga ctccagagtc aagttttatc agagatacag
    19621 cgagcggacg atcaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc
    19681 acggaagcgt cggcgatagt gactacccag ccagacaaag ttactgataa acagcaacga
    19741 agcgccgaca aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc
    19801 cagccacacc atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc
    19861 cagccgcgtc cagataatcg ccagtaacag cgcgcccaga accagcagca ccagtggcag
    19921 aggccagaag aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga
    19981 gaggaaaaac gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc
    20041 gcccagcagt gaggcgcaca gccccgccag caccagatag ctaatggcgt cgaacatcgg
    20101 cgcttgccag gccagtaaca gcaggagaag aagggttaat ggtttaaata cccagcgttg
    20161 ccaggttggc ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat
    20221 aaacgaccaa agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac
    20281 ggtcttgctt ccagtttagt ggcgagggtg gacggatgac aacaccataa ctggcagggt
    20341 atgcttaatt ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta
    20401 ttgttatcat tggcttaatc atcgtcgccg catcgtttcg ttttatgcaa caacgacggg
    20461 aaaaagctga taatgatatg gcaccactcc agcaaaagct ggtgttggtg agcaacaagc
    20521 gggaaaaacc gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca
    20581 gtatgcgcta tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc
    20641 tcgacgccca gcagtaccac gccctgacag tgggcgataa aggtacgctg agctataaag
    20701 gaacgcgctt tgtcagcttt gtaggcgaac aataaggtta ttttttaact ttaaatttct
    20761 tctgccagac cagcagttca aacacgccga aaaggaaaat ccgtaccttt tcggcggtgg
    20821 tcatctgcgg gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca
    20881 tcagcacggt aaaaatcagc gccacgttaa cgaaaatatt cagcggacgt gggaagggat
    20941 gcaccaggtt gaggattaaa aatccccaaa cacagagcat taacaaacga ccaatattaa
    21001 tcagcatcac tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc
    21061 actttttccc gatgtaatga ccagtttgct ggcacagtgg gcagaccgtt ttcgacttcg
    21121 ctttcgacat aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc
    21181 gtctcttcta acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgtg
    21241 cctttttgcg ccaggaatga cattgcgttg ctgttcacca cgcgtgcatt gcctgctttt
    21301 agtgtcgcca gattcttaat taactgctga gaaaccgcgc gatccatctc aatcaacgtg
    21361 gcccccgcag catagcgcga caacgcttcc agccccagcg cgccgctccc ggcgaagcaa
    21421 tccagacatt gggcgtcaac aatgaccgga gccagccagt taaacaacgt ttcgcgtacg
    21481 cggtcggtgg tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac
    21541 tgcccgccaa taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat
    21601 tgctcaatcc gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata
    21661 gactatttca tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca
    21721 acagcgagga gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct
    21781 gggctttggt caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaaac
    21841 ggttgtagaa gaaatcgttc aggcgcaaga gcctgtgaag gcttctgaac aagccgtcga
    21901 agagcagccg caggcgcata ctgaggccga ggcggaaact tttgctgccg acgttgtgga
    21961 agtcactgaa caggttgctg aaagtgaaaa agcgcagcct gaagcggaag tcgttgcaca
    22021 accggaaccg gtcgtagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc
    22081 gttgccggaa gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga
    22141 aaccgtagag attgtcgaag cggcggaaga agaagcggct aaagaagaaa tcaccgacga
    22201 agagctggaa gcacaggcgc tggctgccga agcggcagaa gaagcggtga tagtggttcc
    22261 tccggcagaa gaagagcagc cggttgaaga aattgcccag gagcaggaaa aaccgaccaa
    22321 agaaggtttc ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc
    22381 cggatttatc agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg aggagctgga
    22441 agaacagctg ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaataa tcaccaatct
    22501 gacggaaggc gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa
    22561 agaagagatg ggcgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac
    22621 gccgttcgtg atcctgatgg tgggcgtcaa cggtgtgggt aaaaccacga cgattggtaa
    22681 gctggcgcgt cagtttgagc agcagggtaa atcggtgatg ctggcggcgg gcgatacttt
    22741 ccgtgcagca gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat
    22801 tgcccagcat accggtgcgg attccgcctc tgttatcttc gacgccattc aggcagctaa
    22861 agcgcgtaat atcgacgtcc tgattgccga taccgccgga cgcctgcaga acaaatcgca
    22921 cctgatggaa gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc
    22981 gcatgaagtt atgctgacta ttgatgccag taccgggcag aatgcggtaa gccaggccaa
    23041 actgttccac gaagccgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc
    23101 gaaaggcggg gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg
    23161 tgtcggcgaa cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact
    23221 ttttgcccga gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt
    23281 gggagacagg cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg
    23341 accggtcatt ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg
    23401 cccagcgccg ggaaaatctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa
    23461 gttccgtttc tgcgccgcca gattggcatg attttccagg atcaccatct actgatggac
    23521 cgtactgtct acgataacgt ggcgatcccg ctgattatcg ctggtgccag cggtgacgat
    23581 attcgtcgcc gggtgtcggc ggcactggat aaagtcgggc tactggacaa agcgaagaac
    23641 ttccctattc agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg
    23701 aataagcccg cggtactgct ggcggacgaa ccgactggta acctggacga cgcgttgtcg
    23761 gaagggattc tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt attgatggca
    23821 acgcacgaca tcaacctgat ttcgcggcgt tcctatcgca tgctcaccct gagcgatggt
    23881 cacttgcatg gaggcgtggg ccatgaataa acgcgatgca atcaatcata ttcggcagtt
    23941 tggtgggcgt ctggatcgct tccgtaaatc ggtcggcggc tctggcgacg gcggtcgtaa
    24001 cgcaccgaaa cgcgcgaaat cctcgccaaa accggtaaat cgcaaaacca acgttttcaa
    24061 cgaacaggtg cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc
    24121 cacattttta acggtgatgg ttatcgccat ctccctgacg ctgcccagcg tctgttatat
    24181 ggtgtacaaa aacgttaatc aggcggcgac gcagtattat ccgtcaccgc aaatcactgt
    24241 ttatctgcaa aaaacgctgg acgatgacgc tgctgcgggc gtggtggcac agttgcaggc
    24301 cgagcaaggc gtggagaaag tgaactatct ttctcgtgaa gacgcactgg gtgagttccg
    24361 taactggtct ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt
    24421 ggcggtggtg atcccgaaac tcgattttca ggggacggaa tcactcaata cgctgcgtga
    24481 tcgtatcacg cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg
    24541 tctggcggcg ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg tgttgatggt
    24601 ggcggcagtg ttcctcgtca tcggtaacag tgtacgcctg agtatctttg ctcgtcgtga
    24661 ctccattaac gtgcagaaac tgattggtgc aacggatggt ttcatcctgc gcccgttcct
    24721 gtacggtggc gcattgctgg ggttttctgg cgcattgttg tcactaattt tgtcagaaat
    24781 tctggtactg cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaagtt
    24841 tgatatcaat ggcttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat
    24901 tggctgggtg gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata
    24961 ataaaagcgt gttatactct ttccctgcaa tgggttccgt agcagggaaa gagaccccgt
    25021 tgtctcttcc cggtatttca tctctatgtc acattttgtg cgtaatttat tcacaagctt
    25081 gcattgaact tgtggataaa atcacggtct gataaaacag tgaatgataa cctcgttgct
    25141 cttaagctct ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga
    25201 tttgaatgac tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct
    25261 acatccgggc agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg
    25321 aaaagctgca ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc
    25381 ggtttgttgt tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga
    25441 ttcaggaagg taacatcggc ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg
    25501 tgcgcctggt ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tacgttctgc
    25561 gtaactggcg tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc
    25621 tgcgtaaaac caagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc
    25681 gtgaactggg cgtaaccagc aaagacgtac gtgagatgga atcacgtatg gcggcacagg
    25741 acatgacctt tgacctgtct tccgacgacg attccgacag ccaaccgatg gctccggtgc
    25801 tctatctgca ggataaatca tctaactttg ccgacggcat tgaagatgat aactgggaag
    25861 agcaggcggc aaaccgtctg accgacgcga tgcaaggtct ggacgagcgt agccaggaca
    25921 tcatccgcgc gcgctggctg gacgaagaca acaagtccac gttgcaggaa ctggctgacc
    25981 gttacggcgt ttccgctgag cgtgtgcgcc agctggaaaa gaacgcgatg aaaaaactgc
    26041 gtgctgccat tgaagcgtaa tttccgctat taagcagaga accctggatg agagtccggg
    26101 gtttttgttt tttgggcctc tgtaataatc aatttcccct ccggcaaaac gccaatcccc
    26161 acgcagattg ttaataaact gtcaaaatag ctattccaat atcataaaaa tcgggtatgt
    26221 tttagcagag tatgctgcta aagcacgggt agtcatgcat aaaacgaaat aaagtgctga
    26281 acaacaacat cacaatacac gtaataacca gaagaatggg gattctcagg atgaacataa
    26341 agggtaaagc gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag
    26401 aagatattaa agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc
    26461 aggagtttac cggcgcagag caggcggttg cggatatcaa cgctaaaggc ggcattaaag
    26521 gcaacaaact gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg
    26581 tggcgaacaa agtcgttaac gacggcatta aatatgtgat tggtcacctc tgttcttcat
    26641 caacgcagcc tgcgtctgac atctacgaag acgaaggcat tttaatgatc accccagcgg
    26701 caaccgcgcc ggagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg
    26761 actccgacca ggggccgacg gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta
    26821 ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct ggcgcgagcg gtgcaggacg
    26881 gcctgaagaa agacaatgct aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag
    26941 atttctcaac gctggtggcc cgtctgaaaa aagagaatat cgacttcgtt tactacggcg
    27001 gttatcaccc ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc
    27061 agtttatggg gccggaaggt gtggctaacg tttcgctgtc taacattgcg ggcgaatcag
    27121 cggaagggct gctggtgacc aagccgaaga actacgatca ggttccggcg aacaaaccca
    27181 ttgttgacgc catcaaagcg aaaaaacagg acccaagcgg cgcattcgtt tggaccacct
    27241 acgccgcgct gcaatctttg caggcgggcc tgaatcagtc tgacgatccg gctgagatcg
    27301 ccaaatacct gaaagcgaac tccgtggata ccgtgatggg tccgctgacg tgggatgaga
    27361 aaggtgatct gaaaggcttt gagttcggcg tatttgactg gcacgccaac ggcacggcga
    27421 ccgatgcgaa gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag
    27481 tacgttgctt catgccggat gcggcgtaaa cgccttatcc ggcctacaag atccaaagaa
    27541 atcagtaaat tgcaacacac attgtaggcc tgataagcgt agcgcatcag gcaatttctc
    27601 gttggtcatc acttttgaaa tcaacttgac gattaacgct tctcccagcc gccctgttgc
    27661 gccgtaaacc ccagcgcctg cataaacgcc gtcatcacac cgcgatcttc cacgcctgca
    27721 tccgccatcc accagcatga aacggcagga ttgttacgca aaacctcttc cagcagatat
    27781 tgccccacac cgcgacggcg ggtgacttcc cgcacgcgca gggaatccag tgctccctcg
    27841 gtgccgctta aggttacccg cacggccgct agcaggcgct cgttaaaacg cgcggcgtag
    27901 atgcggtggt tatcgtcaac ctgtaaagag gaaggggaat actccggcca gattttttgc
    27961 aggtcaatcc ggtcctggtc gctaaagttt tctaatcgaa ttatggtcag cttcatgggt
    28021 aacccgtgta aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc
    28081 tcttttttat ttcttttggc aggtgattaa ttttttaaca gcaataatta caaagttaaa
    28141 acattagaga atgaaaaata c
//
LOCUS       DAPGWD010000048        28118 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPGWD010000048 DAPGWD010000000
VERSION     DAPGWD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.048"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..797
                     /locus_tag="RHX88_002014"
     CDS             72..797
                     /locus_tag="RHX88_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV2230705.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            755..1428
                     /gene="bglJ"
                     /locus_tag="RHX88_002015"
                     /pseudo
     CDS             755..1428
                     /gene="bglJ"
                     /locus_tag="RHX88_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(1466..2254)
                     /gene="fhuF"
                     /locus_tag="RHX88_002016"
     CDS             complement(1466..2254)
                     /gene="fhuF"
                     /locus_tag="RHX88_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDV2230706.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2395..2631
                     /locus_tag="RHX88_002017"
     CDS             2395..2631
                     /locus_tag="RHX88_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDV2230707.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2670..2756)
                     /locus_tag="RHX88_002018"
     tRNA            complement(2670..2756)
                     /locus_tag="RHX88_002018"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2720..2722),aa:Leu,seq:cag)
     gene            complement(3024..4055)
                     /gene="rsmC"
                     /locus_tag="RHX88_002019"
     CDS             complement(3024..4055)
                     /gene="rsmC"
                     /locus_tag="RHX88_002019"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDV2230708.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4158..4571
                     /gene="holD"
                     /locus_tag="RHX88_002020"
     CDS             4158..4571
                     /gene="holD"
                     /locus_tag="RHX88_002020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDV2230709.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            4540..4986
                     /gene="rimI"
                     /locus_tag="RHX88_002021"
     CDS             4540..4986
                     /gene="rimI"
                     /locus_tag="RHX88_002021"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDV2230710.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5001..5678
                     /gene="yjjG"
                     /locus_tag="RHX88_002022"
     CDS             5001..5678
                     /gene="yjjG"
                     /locus_tag="RHX88_002022"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDV2230711.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5769..7358
                     /gene="prfC"
                     /locus_tag="RHX88_002023"
     CDS             5769..7358
                     /gene="prfC"
                     /locus_tag="RHX88_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDV2230712.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7751..8356
                     /gene="osmY"
                     /locus_tag="RHX88_002024"
     CDS             7751..8356
                     /gene="osmY"
                     /locus_tag="RHX88_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDV2230713.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8483..8644
                     /locus_tag="RHX88_002025"
     CDS             8483..8644
                     /locus_tag="RHX88_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDV2230714.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8766..9839
                     /locus_tag="RHX88_002026"
     CDS             8766..9839
                     /locus_tag="RHX88_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDV2230715.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9836..10618
                     /locus_tag="RHX88_002027"
     CDS             9836..10618
                     /locus_tag="RHX88_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2230716.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QANRPEQAARVFAVLCELRSEPADEIAEVLLNNTYAVFSVSG"
     gene            complement(10731..11594)
                     /locus_tag="RHX88_002028"
     CDS             complement(10731..11594)
                     /locus_tag="RHX88_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDV2230717.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(11566..13116)
                     /locus_tag="RHX88_002029"
     CDS             complement(11566..13116)
                     /locus_tag="RHX88_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDV2230718.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13374..14153
                     /gene="deoC"
                     /locus_tag="RHX88_002030"
     CDS             13374..14153
                     /gene="deoC"
                     /locus_tag="RHX88_002030"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDV2230719.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14280..15602
                     /gene="deoA"
                     /locus_tag="RHX88_002031"
     CDS             14280..15602
                     /gene="deoA"
                     /locus_tag="RHX88_002031"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDV2230720.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15654..16877
                     /gene="deoB"
                     /locus_tag="RHX88_002032"
     CDS             15654..16877
                     /gene="deoB"
                     /locus_tag="RHX88_002032"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV2230721.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            16957..17676
                     /gene="deoD"
                     /locus_tag="RHX88_002033"
     CDS             16957..17676
                     /gene="deoD"
                     /locus_tag="RHX88_002033"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDV2230722.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(17951..18100)
                     /locus_tag="RHX88_002034"
     CDS             complement(17951..18100)
                     /locus_tag="RHX88_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230723.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(18132..19148)
                     /gene="lplA"
                     /locus_tag="RHX88_002035"
     CDS             complement(18132..19148)
                     /gene="lplA"
                     /locus_tag="RHX88_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDV2230724.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(19176..19820)
                     /locus_tag="RHX88_002036"
     CDS             complement(19176..19820)
                     /locus_tag="RHX88_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDV2230725.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            19926..20894
                     /gene="serB"
                     /locus_tag="RHX88_002037"
     CDS             19926..20894
                     /gene="serB"
                     /locus_tag="RHX88_002037"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDV2230726.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            20943..22325
                     /gene="radA"
                     /locus_tag="RHX88_002038"
     CDS             20943..22325
                     /gene="radA"
                     /locus_tag="RHX88_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDV2230727.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            22346..23578
                     /gene="nadR"
                     /locus_tag="RHX88_002039"
     CDS             22346..23578
                     /gene="nadR"
                     /locus_tag="RHX88_002039"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDV2230728.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(23885..25552)
                     /gene="ettA"
                     /locus_tag="RHX88_002040"
     CDS             complement(23885..25552)
                     /gene="ettA"
                     /locus_tag="RHX88_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDV2230729.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            25763..27700
                     /gene="sltY"
                     /locus_tag="RHX88_002041"
     CDS             25763..27700
                     /gene="sltY"
                     /locus_tag="RHX88_002041"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDV2230730.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27790..28116
                     /gene="trpR"
                     /locus_tag="RHX88_002042"
     CDS             27790..28116
                     /gene="trpR"
                     /locus_tag="RHX88_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDV2230731.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
BASE COUNT         6639 a         7185 c         7690 g         6604 t
ORIGIN      
        1 ctggaaacat ttaaggaatg aatatcctga ataaaccatc ataattttta gacagtggat
       61 gtggaggaaa tatgttgcca ggatgctgca aaaatggaat tgttatcagt aaaatacctg
      121 ttatgcaagc agggttaaaa gaggtcatga ggactcactt ccctgaatat gaaataatat
      181 ccagcgcctc tgcggaggac cttaccttat tacaattacg tcgttccgga ttagtcattg
      241 ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat tattctttaa
      301 tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg tattcccagg
      361 cagtagaatt gctcatgtgc cctacggcga cgttattgtc tgatgttgaa cccattgaga
      421 atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc gccatgctga
      481 cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt ctcactcttt
      541 cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag atagcttcgt
      601 tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg cgtcgactgg
      661 caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt gcaagagaac
      721 ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa ttaagaagag
      781 aaatgtcgca ctcatagaaa aatgcgtcat gagcagtatc ggtattgaga gtttattcag
      841 aaagtttgcg ggtaaccctt ataagctcca tacctatacc agtcaggagt catttcagga
      901 tgccatgtcg cggatctcat ttgcggcggt cattttttct ttttctgcca tgagaagtga
      961 gcgcagagag ggattatctt gcctgactga actggcgatt aagtttccgc gtacccggcg
     1021 tttagttatt gcggatgatg atattgaagc tcggctgatt ggttcattgt cgccatcacc
     1081 gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg aactcttttt
     1141 gtcattaaat ggtgtacgtc aggcgaccga ctgaacaatc agtggtacat taaccaaagc
     1201 cggacgctaa gcccgacgga gagagaaata ttgcgcttta tgtcgcgtgg ctactcaatg
     1261 acacaaattg ccgagcagct taaacgcaat atcaaaacga tccgtgcaca taaatttaat
     1321 gtgatgtcga aactgggcgt cagttctgat gcagggttgt tggaggccgc agatattctg
     1381 ttatgtatgc ggcattgcga agcaagtaat gtgttgcatc cctattaatc cgcatgatgc
     1441 cgggtttact tccccggcag tgctttcatt tcagcgtaca atcgccacat tgctgcacat
     1501 ccggtaagcg ataacgctgg cagcaggtcc ggcgcaccag caggccgtcg cgtagcacca
     1561 cggtacgcca gagtggatta tcttcaccgg tagtaagcgt tttctcaaaa aagagggcat
     1621 ggcgcagcga ttcaaccgtc gcctcgccga gcagttgctt catctcagta agataccagt
     1681 tgatcaaata accggtatta ctccagataa gtttgccgtt gatctctccg gtcgcttcta
     1741 gtgcctgcac aaccggaacc agcgcctggc tgattaacct ttccattcgc tgctgcggcg
     1801 aatgtggtgt tgcgttttta tcttcacaca catcgaccca gaaacaggcg gcacggccgg
     1861 tttcgtgaaa ctcagcatgg aaatgttccg gcgacacatc taatgccttt tcctgcgtca
     1921 gtagcgccag cattaatggt ggcaccatca ggccgatata ccattgtgcc catagtgaga
     1981 tcagcggttt gttctcgcgg atcatcgttg gttggttgcg atagatatga tcggaataga
     2041 ccgcgagtag agaacttagc gcattcggtg atgaccattg cgccagcgtc atggcgttaa
     2101 gcggagcggg ttcatccagg cggataaact ccagcaaatg ttcacgatgt tcggcgatcg
     2161 tcgcacgtac ggcttgtgca agcccagcat cctgtggctg gagatgcgtt cgccagataa
     2221 catcttcata gagcggtgcg gaacgatagg ccataatcgg gatagtaatc taaatgataa
     2281 tgattgctaa tcctagcgat aggtttaccc gatagcaagg gatttctctg gcttgcaaat
     2341 gataaaaatt atcatatgat attggttatc attatcaatg aaagagatga aaccatgttg
     2401 caacgtacgc tgggcagtgg ctggggagtg ttgctgccag gattgctgat tgcagggctg
     2461 atgtatgcgg atttatcacc ggatcagtgg cggattgtca ttctgatggg attagtattg
     2521 acgtcggtga tgctgtatca caaacagtta cggcattacg ttttgctacc atcgtgcctg
     2581 gcgcttattg ctgccatcat gctgatgata atgaatttga atcagggatg aaaaatcaag
     2641 gaagaaacaa gaaaggaagt aaagataatt ggtgcgaggg gggggacttg aacccccacg
     2701 tccgtaagga cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcatac
     2761 catcaattct taaaaagaat tgctaccacg gaggcgcatt ctagtggttt tcagcttttc
     2821 gtcaatagtt aattatcgcc ggaggtgtaa ttgctggaaa aatgtccatc aggaaactag
     2881 cgtgcaggtt tggtaggcat gcgggggcag atgccagatg cgacgctggc gcgtcttatc
     2941 tggcctacga agggctaacg tgcaggtttt gtaggtcgga taaggcgttc acgccgcatc
     3001 cgacacggta ttcggcgaga taattaacct ttcttcgcct ggcgggtcat aatggcgcga
     3061 tacaccttga aacgcccggt ttgcgcaatc acttcatgga agccaaatgt ctcatccagc
     3121 acgtccgggt aaggcaggaa ggcgttcgct acaattcgca gctcgccgcc gctattaaga
     3181 tgacgcaccg cgccgcgaat cagcgtttgc gccgcatcca ggctggtttg catcccatcg
     3241 tggaacggcg ggttggagat gatcatatca aaacgacctt tcacctcgga aaagacgttg
     3301 ctggcaaaga cttcaccttc aacacagttg gccgcaagtg ttgcgcggct ggcttctacc
     3361 gctggcgcag agacatcgca caaggtgaga cgaattttcg gcgaatggcg cgcaaaggca
     3421 actgaaagca cccccgcgcc acagccgaca tccagcactt tacctttcgt gtgcggagtt
     3481 aacgtcgaga gcagcaactg gctaccgaca tccagaccgt cgcggctaaa cacgccaggc
     3541 agcgttttga ccgtcaggcc atcgacgctg tattcgcccc agaatttatc ggcatcaaat
     3601 accggctgtt tttccagacg accaaaatag aggccacagc gacgagcgct gtcgacttta
     3661 ttcaacggcg cataatctgc cagcatctgc tcggcgctgc gcacgccgct gcggttctcg
     3721 ccaacgacaa aaatatccgt ccccaccggc agcagagaaa gtaaattcat caactggaac
     3781 tgggcttccg gtttgttctt cggccagtag taaatcagcg tatcgcaatc tgcgacgtca
     3841 tccgccgtgg cgaccagact gaaacgggcg ttatccccca tctggcggct taatacctgc
     3901 cagtggtgga attgctgggt atgagcacgg ctggccgcgg tatctaaacg cgcgggcagg
     3961 tcatcctgta agtctccggc aaacagaata cggctttgtt cgaaatcatc actgtgacgc
     4021 agcaagactt cacttgccgg ggtaaatgca gacatggaat gctcctcaat tgatactggc
     4081 ggcgattata gccatatgtt ggcgcggtat cgacgaattt gctatatttg cgcccctgac
     4141 aacaggagcg attcgctatg acatcccgac gagactggca gttacagcaa ctgggcatta
     4201 cccagtggtc gctgcgtcgc cctggcgcgt tgcaggggga gattgccatt gcgatcccgg
     4261 cacacgtccg tctggtgatg gtggcaaacg atcttcccgc cctgactgat cctttagtga
     4321 gcgatgttct gcgcgcatta accgtcagcc ccgaccaggt gctgcaactg acgccagaaa
     4381 aaatcgcgat gctgccgcaa ggcagtcgct gcaacagctg gcggttgggt actgacgaac
     4441 cgctatcact ggaaggcgct caggtggcat caccggcgct caccgattta cgggcaaacc
     4501 caacggcacg cgccgcgtta tggcaacaaa tttgcacata tgaacacgat ttcttccctc
     4561 gaaacgactg atttaccggc ggcttaccac attgaacaac gcgcccacgc ctttccgtgg
     4621 agtgaaaaaa cctttgccag caaccagggc gagcgttatc tcaactttca gttaacgcaa
     4681 aacggcaaaa tggcggcgtt tgcgattacg caagtggtgc tggatgaagc tacattgttc
     4741 aatattgcgg tcgatcctga ctatcagcgt cagggattgg gaagggcgct gctggaacat
     4801 ctgatcgacg aactggaaaa acgcggcgtg gcgacactat ggctggaagt ccgtgcttca
     4861 aacgctgccg ccattgccct gtacgaaagt ttaggcttta acgaggcgac gattcgccgc
     4921 aattactacc ccaccacgga cggtcgcgaa gacgccatca tcatggcgtt gccaatcagt
     4981 atgtaagaca aggtggaata atgaagtggg actggatttt ctttgatgcc gatgaaacgc
     5041 tgtttacctt tgactcattc accggcctgc agcggatgtt tcttgattac agcgtcacct
     5101 ttaccgctga agattttcag gactatcagg ccgttaacaa gccactgtgg gtggattatc
     5161 aaaacggcgc gatcacttca ttacagcttc agcacgggcg ttttgagagc tgggccgaac
     5221 ggctgaaagt tgaagcaggc ttgcttaacg atgcctttat taatgcgatg gcggaaatct
     5281 gcacgccgct gccgggcgcg gtttctctgc ttaacgccat tcgtggcaac gccaaaatcg
     5341 gcatcatcac caacggcttt agcgccttgc agcaagtgcg tctggaacgc acgggcctgc
     5401 gtgattactt cgatttgctg gtgatttccg aagaagttgg cgttgccaaa ccgaataaga
     5461 aaattttcga ttatgcgctg gaacaggcgg gcaatcctga ccgttcacgc gtgctgatgg
     5521 ttggcgacac tgccgagtcc gatattctcg gtggcatcaa cgccgggctt gcgacctgct
     5581 ggctgaatgc gcaccatcgc gagcaaccag aaggcatcgc gcccacctgg accgtttctt
     5641 cgttgcacga actggagcag ctcctgtgta aacactgatt gcctcccccc cgttgatggg
     5701 taaaatagcc gcaatttttc gttttcaaca agcgcggcgc gatgccgctt actcaagaag
     5761 aaagaattat gacgttgtct ccttatttgc aagaggtggc gaagcgccgc acttttgcca
     5821 ttatttctca cccggatgcc ggtaagacta ccatcaccga gaaggtgctg ctgttcggac
     5881 aggccattca gaccgccggt acagtaaaag gccgtggttc caaccagcac gctaagtcgg
     5941 actggatgga gatggaaaag cagcgtggga tctccattac tacgtctgtg atgcagtttc
     6001 cgtatcacga ttgcctggtt aacctgctcg acaccccggg gcacgaagac ttctcggaag
     6061 atacctatcg taccctgacg gcggtggact gctgcctgat ggttatcgac gctgcaaaag
     6121 gtgttgaaga tcgtacccgt aagctgatgg aagttacccg tctgcgcgac acgccgatcc
     6181 tcacctttat gaacaaactt gaccgtgata tccgcgaccc gatggaactg ctcgatgaag
     6241 ttgagaacga gctgaaaatc ggctgcgcac cgatcacctg gccgattggc tgcggcaagc
     6301 tgtttaaagg cgtttaccac ctttataaag acgaaaccta tctctatcag agcggtaaag
     6361 gccacacgat tcaggaagtc cgtattgtta aagggctgaa taacccggat ctcgacgctg
     6421 cggttggtga agatctggca cagcagctac gtgacgaact ggaactggtg aaaggcgcgt
     6481 ctaacgagtt cgacaaagaa ctgttccttg cgggcgaaat cactccggta ttcttcggta
     6541 ctgcgctggg taacttcggt gttgatcata tgctggatgg cctggtggag tgggctcctg
     6601 cgccgatgcc gcgtcagact gatacccgta ccgtagaggc gagcgaagac aaatttaccg
     6661 gcttcgtatt taaaattcag gccaacatgg acccgaaaca ccgcgaccgc gtggcgttta
     6721 tgcgcgtagt atccggtaaa tatgaaaaag gcatgaagct gcgccaggtg cgtactgcga
     6781 aagatgtggt tatctccgac gcgctgacct ttatggcggg cgaccgttcg cacgttgaag
     6841 aagcgtatcc gggcgatatc ctcggcctgc acaaccacgg cactattcag atcggcgaca
     6901 cctttaccca gggtgagatg atgaagttca ccggtattcc gaacttcgca ccagaactgt
     6961 tccgtcgtat ccgcctgaaa gatccgctga agcaaaaaca gctgctcaaa gggctggtac
     7021 agctttccga agagggcgcg gtgcaggtgt tccgtccgat ctccaacaac gatttgatcg
     7081 ttggtgctgt tggtgtgctg cagtttgatg tggtggtatc gcgcctgaag agcgaataca
     7141 acgttgaagc agtatatgaa tcagtcaacg ttgccactgc ccgctgggta gaatgtgcgg
     7201 acgcgaagaa attcgaagag ttcaagcgta agaacgaaag ccaactggcg cttgatggcg
     7261 gcgataacct cgcttacatc gctaccagca tggtcaacct gcgcctggcg caggaacgtt
     7321 atcccgacgt tcagttccac cagacccgcg agcattaatt cttgcctcca gggcgcggta
     7381 gccgctgcgc cctgtcaatt tcccttcctt attagccgct tacggaatgt tcttaaaaca
     7441 ttcacttttg cttatgtttt cgctgatatc ccgagcggtt tcaaaattgt gaactatatt
     7501 taacaaagta atgacatttc tgacggcgtt aaataccgtt caatgcgtag atatcagtat
     7561 ctaaagccgt cgattgtcat tctaccgata ttaataactg attcagaggc tgtaatggtc
     7621 gttattcatc acccatcgct tttgtgatgg cgaccattga cttctgtaga gggtgaagtc
     7681 tctccctatt cagcaatgca acctcgtgtt gccaggctca aattacgagc aaacatacag
     7741 gaataaatcg atgactatga caagactgaa gatttcgaaa actctgctgg ctgtaatgtt
     7801 gacctctgcc gtcgcgaccg gctctgccta cgcggaaaac aacgcacaga ctaccaatga
     7861 aagcgcaggg caaaaagtcg atagctctat gaataaagtc ggtaatttca tggatgacag
     7921 cgccatcacc gcgaaagtga aggcggcgct ggtggatcat gacaacatca agagcaccga
     7981 tatctctgtc aaaaccgatc aaaaagtcgt gaccctgagc ggtttcgttg aaagccaggc
     8041 tcaggccgaa gaggcagtga aagtggcgaa aggcgttgaa ggggtgacct ctgtcagcga
     8101 caaactgcac gttcgcgacg ctaaagaagg ctcggtgaag ggctacgcgg gtgacaccgc
     8161 caccaccagt gaaatcaaag ccaaactgct ggcggacgat atcgtccctt cccgtcatgt
     8221 gaaagttgaa accaccgacg gcgtggttca gctctccggt accgtcgatt cccaggcaca
     8281 gagcgaccgt gctgaaagta tcgccaaagc ggtagatggt gtgaaaagcg ttaaaaatga
     8341 tctgaaaact aagtaattcg tcgtaattcg tcctcccgaa ctttgtcggg aggcgcgatg
     8401 tgcaccacac taaaaatatc gcattgagtg attttcacgc tcatatcaag cggtgacatt
     8461 aactatggta aaggagacgc ttatgtttcg ttggggcatc atatttctgg ttatcgcgtt
     8521 aatcgccgcc gcacttgggt ttggtggtct ggccggtacc gctgcaggcg cagctaaaat
     8581 tgtctttgtc gtcgggatta ttctgttcct ggtgagtttg ttcatgggcc gaaaacgacc
     8641 ctagatttca gaccattaag aaaattttat ccaaagccag tccagcggac tggcttttgc
     8701 ggttttagcg aataataaat tgcgttactt tgtcattctt tcacaacaaa aacaggaagg
     8761 cagaggtggg gcagcgaata cctgtaacgc ttggtaatat tgcgccgttg tcgctaaggc
     8821 cgttccagcc tggacgaata gctctggtgt gcgaaggcgg cggacagcgt ggaattttca
     8881 cggctggcgt gctggatgag tttatgcgcg cgcagtttaa tcctttcgat ctttatctcg
     8941 gcacatctgc cggggcgcag aacctctcgg cgtttatctg caatcagccc ggttacgcgc
     9001 gcaaagtcat catgcgctat accacaaaac gcgaattttt cgatccattg cgctttgtcc
     9061 gtggaggaaa tcttatcgat ctcgactggc tggtggaggc caccgcaagc cagatgccgt
     9121 tgcaaatgga taccgccgcg cggttgtttg acagcggcaa atcgttttat atgtgcgcct
     9181 gtcgtcagga tgactacgcg cctaattact ttttaccaac caaacaaaac tggctggatg
     9241 tgattcgcgc ctccagtgcg atacctggct tttatcgtag cggagtgtcg ctggaaggca
     9301 ttaactacct ggatggcggg atcagtgatg cgattccggt taaagaggcg gcaaggcagg
     9361 gcgctaaaac gttggtcgtc atccgcactg tgccgtcaca aatgtactac acgccgcagt
     9421 ggttcaaacg catggaacgc tggctgggtg acagtagcct gcagccgctg gtcaatctgg
     9481 tgcagcatca tgaaaccagc tatcgtgaca ttcagcaatt tattgagaaa ccaccgggca
     9541 agctgcggat attcgagatt tatccaccga agccattaca tagtatcgcg cttggcagtc
     9601 ggattccggc gctgcgtgaa gactataaac ttgggcgttt atgcggtcgt tatttcctcg
     9661 ccacggttgg caagctatta actgaaaaag cgccgcttac ccgccatctg gtgccagtgg
     9721 tgacgccgga atcgatcgtt attccgcctg cgccagtcgc caacgatacg ctggttgccg
     9781 aagtgagcga tgctccgcag gcgaacgacc cgacatttaa caatgaggat ctggcttgat
     9841 ttgccgtttt atcgataccc actgccattt tgatttcccg ccgtttagtg gcgatgaaga
     9901 ggccagcctg caacgcgcgg cacaagcggg cgtaggcaag atcattgttc cggcgacaga
     9961 ggcggaaaat tttgcccgcg tgcaggcgtt agcggaaaaa tatcaaccgc tttatgccgc
    10021 attggggttg catccgggga tgttggaaaa acatagcgat gtgtctcttg atcagctaca
    10081 gcaggcgctg gaaaggcgtc cggcgaaggt ggtggcggtg ggggagatcg gtctggatct
    10141 cttcggcgac gatccgcaat ttgagaggca gcagtggtta ctcgacgaac agctgaaact
    10201 ggcgaaacgc tacgatctgc cggtgatcct tcattcccgg cgcacacacg acaaactggc
    10261 gatgcatctt aaacgccacg atttatcgcg cactggcgtg gttcacggtt tttccggcag
    10321 cctgcaacag gccgaacggt ttgtacagct gggctacaaa atcggcgtag gcggtactat
    10381 cacctatcca cgcgccagta aaacccgcga tgtcatcgca aaattaccgc tggcatcgtt
    10441 gttgctggaa accgacgcgc cggatatgcc gctcaatggt tttcaggggc aggctaaccg
    10501 cccggaacag gccgcccggg tatttgccgt gctgtgcgag ttgcgctcgg aaccggcgga
    10561 tgagattgcc gaagtgttgc ttaataatac gtatgcggtg tttagcgtta gtgggtagtt
    10621 tgcccttcgt aggcagcatc cggcagtcgg cgcataatgc ctgatgcgac gcttgtcgcg
    10681 tcttatcatg cctacaggtt tgtgccgaac cgtaggccgt atccggcatg tcacaaatag
    10741 agcgccggaa atatcaaccg gctcaccccg cgcaccttta acgcatcagc caacggctca
    10801 acgtcttccg gcgtggcgct cgcccagctt tgcgcctcgc catacacgcc gtgggcatga
    10861 aacgcattca ggcgaaccgg aacatcgcca agtctcttga taaacgtcgc cagctcatcg
    10921 atgtgatgca aataatccac ctggtctgga attaccagca aacgcagttc cgccagctta
    10981 ccgcgctctg ccagcaaaca gatgctgcgc ttaatctgct gattatcgcg tccggttaat
    11041 tgctgatgac attcgctccc ccacgctttg agatcgagca tcgcgccgtc gcacaccggg
    11101 agtaattttt cccagccggt ttcgctcaac ataccgttac tgtccacgag gcaggtgagg
    11161 tggcgcagtt gcggatcgtt tttgatggct gtaaacagcg ccaccacaaa cggcagttgg
    11221 gtcgtggctt cgccgccgct taccgttatc ccttcaataa acagcaccgc tttgcggaca
    11281 tggctaagca cttcgtccac gctcatggat tgcgccattg gcgtggcatg ttgcggacac
    11341 ctcttcaggc aggtatcaca ctgctcgcaa accacagcgt tccacaccac tttgccgtca
    11401 acaatctgca acgcctgatg cggacactgt ggcacgcact ccccacagtc attgcaacgt
    11461 cccatcgtcc acggattgtg acagtttttg cagcgcagat tgcagccctg caaaaacaga
    11521 gccagacgac tgcctggccc gtcaacgcag gagaagggga taatcttact gactaaagcg
    11581 catctgctgt tcatggctta tcacgcgcgg ctggcgttcc agaatacgag tgttgcgtgc
    11641 ggcttcttcg cccagccagg tggtgttggt gcgtgaacct tcggcgcgat atttttctaa
    11701 gtccgacaaa cgcaccatat aaccggtaac gcgaaccaga tcgttaccgc tgacattggc
    11761 ggtaaattca cgcattccgg ctttaaaggc accgaggcaa agctgtacca gtgcctgcgg
    11821 gttacgtttg atggtttcgt cgagcgtcag aatgtcgctg atgccggaat aataataagc
    11881 atgatgcggg gcgacagttt gcagatgggt gatcggatct ggctcatcgc catacggcaa
    11941 acgcgcgccc ggcgtggtgc cgatatcgga actgatcccc gactgtgcgt gtaacatggc
    12001 gcgtttttgc cagccatatt tcacgggggt attggcgaca aactccgcca gttgcgcgct
    12061 gatgcgataa cccacttcat ttgcggcggc ttctttaccg tagcgcgcgg caatcccttc
    12121 tttcttacac agcaagttaa ccgcttccgc cagcccatac atgccaaaca ttggcacaaa
    12181 acgttcaggg ttaatcagcc cttctttcac caggaagcta ttctcaaaga agtgtgattg
    12241 ttgatagagg aattcacacc gcgcatcgat gatggcgatc tgctgctggc agtagtgcgg
    12301 tagagtgcgc gtaaagaagt catccagcga ttcgctgcgc tcggcaatgg ctttcaggtt
    12361 aaggcgtacc agcgtgctgc caccacccgc cagcggcagt gagttgtaac agctcacaat
    12421 cccgtagccc ccttttgtga aaattttatc atgcaccgga ccgttggcga tgtgcggttt
    12481 gctacattca cagatgttct tcgccacttc cagcagcagg tcatcagggg tgatttcagg
    12541 atcgtagata aaggtcaggt tcggtgaaac ctgcttcaac tctgcgtctg cacgtaagat
    12601 cgcgcgggta atgggcgaat cagacgggcc gatattggcg tgcataaagg cgtctggcag
    12661 ggttctgtcg aggtaacgcc agaaacgttt tattcgaaca tcgatctcgt cttgtgttag
    12721 aattctaaca tacggttgca acaacgcatc cagttgcccc aggtagaccg gcatcgatgt
    12781 gaccgacggt acgtggtggt aaagaatggt cagcagagag agtgcgtcat caagatcttt
    12841 cgcgccttcc agctccagcc attcggaacc gttcgccaga aaacgggcgt aatcgggtaa
    12901 gacatagcgc ggtttgtacg gcgcatgacc ttcaaacata tcgcagatta caccctcatc
    12961 cagcgcgcgg cgggcttcgg caggaagctg cggataaggc agattgtttt ctgcttccag
    13021 agccagaaaa tggcgcttct gctccgggct aagcactggg ctggtgacaa tttgctggca
    13081 acgttgttgc agtgcatttt catgagaagt gggcatcttc ttttcctttt atgccgaagg
    13141 tgatgcgcca ttgtaagaag tttcgtgatg ttcactttga tcctgatgcg tttgccacca
    13201 ctgacgcatt catttgaaag tgaattattt gaaccagatc gcattacagt gatgcaaact
    13261 tgtaagtaga tttccttaat tgtgatgtat atcgaagtgt gttgcggagt agatgttaga
    13321 atactaacaa actcgcaagg tgaattttat tggcgacaag ccaggagaat gaaatgactg
    13381 atctgaaagc aagcagcctg cgtgcactga aattgatgga cctgaccacc ctgaatgacg
    13441 acgacaccga cgagaaagta attgctctgt gtcatcaggc caaaactccg gtcggcaata
    13501 ccgccgctat ctgtatctat cctcgcttta tcccgattgc tcgcaaaacg ctgaaagagc
    13561 agggcacccc ggaaatccga atcgctacgg taaccaactt cccacacggt aacgatgaca
    13621 tcgaaatcgc gctggcagaa acccgtgcgg caatcgccta cggtgctgat gaagttgacg
    13681 ttgtgttccc gtaccgcgcg ctgatggcgg gtaacgagca ggttggtttt gacctggtga
    13741 aagcctgtaa agaggcttgc gcggcagcga atgtactgct gaaagtgatc atcgaaaccg
    13801 gcgaactgaa agacgaagcg ctgatccgta aagcgtctga aatctccatc aaagcgggtg
    13861 cggacttcat caaaacctct accggtaaag tggctgtgaa cgcgacgccg gaaagcgcgc
    13921 gcatcatgat ggaagtgatc cgtgatatgg gcgtagaaaa aaccgttggt ttcaaaccag
    13981 cgggcggcgt gcgtactgcg gaagatgcgc agaaatatct cgccatcgca gatgaactgt
    14041 tcggtgctga ctgggcagat gcgcgtcact accgctttgg tgcttccagc ctgctggcaa
    14101 gcctgttgaa agcgctgggc cacggtgacg gtaagagcgc cagcagctac taagtaagat
    14161 gctttacgcc tgatgcgctg cgcttatcag gcctacgaga cgtatctacc cgtaggccgg
    14221 ataaggcgta gacgcatccg gcaaaagccg cctcatactc ttttcctcgg gaggttacct
    14281 tgtttctcgc acaagaaatt attcgtaaaa aacgtgatgg tcatgcgcta agcgatgaag
    14341 aaattcgttt ctttatcaac ggtattcgcg acaacactat ctccgaaggg cagattgccg
    14401 ccctcgcgat gaccattttc ttccacgata tgacaatgcc tgagcgtgtc tcgctgacca
    14461 tggcgatgcg agattcagga accgttctcg actggaaaag cctgcatctg aatggcccga
    14521 ttgttgataa acactccacc ggcggcgtcg gcgatgtgac ttcgctgatg ttggggccga
    14581 tggtcgcagc ctgcggcggc tatattccga tgatctccgg tcgcggcctc ggtcatactg
    14641 gcggtacgct cgacaaactg gaatccatcc ctggcttcga cattttcccg gatgacaacc
    14701 gtttccgcga aattattaaa gacgtcggcg tagcgattat cggccagacc agctcactgg
    14761 ctccggcgga taaacgtttc tacgcgaccc gtgatattac cgcaaccgtg gactccatcc
    14821 cgctgatcac tgcctcgatc ctggcgaaga aactggcgga aggtctggat gcgctggtga
    14881 tggacgtgaa agtgggtagc ggcgcgttta tgccgaccta cgaactctct gaagcccttg
    14941 ccgaagcgat tgttggcgtg gctaacggcg ctggcgtgcg caccaccgcg ctgctcaccg
    15001 atatgaatca ggtactggcc tccagtgcag gtaacgcggt tgaagttcgt gaagcggtgc
    15061 agttcctgac gggtgaatat cgtaacccgc gtctgtttga tgtcacgatg gcgctgtgcg
    15121 tggagatgct tatctccggc aaactggcga aagatgacgc cgaagcgcgc gcgaaattgc
    15181 aagcagtgct ggacaacggt aaagcggcag aagtctttgg tcgtatggta gcggcacaaa
    15241 aaggcccaac cgacttcgtt gagaactacg cgaagtatct gccgacagcg atgctgacga
    15301 aagcagtcta tgctgatacc gaaggttttg tcagtgaaat ggatacccgc gcgctgggga
    15361 tggcagtggt tgcaatgggc ggcggtcgtc gtcaggcatc tgacaccatt gattacagcg
    15421 tcggctttac tgatatggcg cgtctgggtg accaggtaga cggtcagcgt ccgctggcgg
    15481 ttatccacgc gaaagacgaa aacagctggc aggaagcggc gaaagcggtg aaagcggcaa
    15541 ttaaacttgc cgataaagca ccggaaagca caccaactgt ctatcgccgt atcagcgaat
    15601 aacggtatac tgatctgatc atttaaattt gaagcactga gtacggagaa catatgaaac
    15661 gtgcatttat tatggtgctg gactcattcg gcatcggcgc tacagaagat gcagaacgct
    15721 ttggtgacgt cggggctgac accctgggtc atatcgcaga agcttgtgct aaaggcgaag
    15781 ctgataacgg tcgtaaaggc ccgctcaatc tgccaaatct gacccgtctg gggctggcga
    15841 aagcacacga aggttctacc ggtttcattc cggcgggaat ggacggcaac gctgaagtta
    15901 tcggcgcgta cgcatgggcg cacgaaatgt catccggtaa agataccccg tctggtcact
    15961 gggaaatcgc cggtgtcccg gttctgtttg agtggggata cttctccgat cacgaaaaca
    16021 gcttcccgca agagctgctg gataaactgg ttgaacgcgc taacctgcct ggttatctcg
    16081 gtaactgcca ctcttccggt acggtcattc tggatcagct gggcgaagag cacatgaaaa
    16141 ccggcaagcc gattttctat acctccgctg actccgtgtt ccagattgcc tgccacgaag
    16201 aaacctttgg cctggataaa ctctacgaac tgtgcgaaat cgcccgtgaa gagctgacca
    16261 acggcggcta caacatcggt cgtgttatcg ctcgtccgtt tattggcgac aaagccggta
    16321 acttccagcg taccggtaac cgtcacgacc tggctgttga gccgccagca ccgaccgtgc
    16381 tgcagaaact ggttgatgaa aaacacggtc aggtggtttc tgtcggtaaa attgcagaca
    16441 tctacgccaa ctgcggtatc accaagaaag tgaaagcaac tggcctggac gcgctgtttg
    16501 acgccaccat caaagagatg aaagaagcgg gtgataacac catcgtcttc accaacttcg
    16561 ttgacttcga ctcttcctgg ggccaccgtc gcgacgtcgc aggttatgcg gcgggtctgg
    16621 aactgttcga ccgccgtctg ccggagctga tgtctctgct gcgcgatgac gacatcctga
    16681 tcctcactgc tgaccacggt tgtgatccga cctggaccgg tactgatcac acgcgtgaac
    16741 acattccggt actggtttac ggcccgaaag taaaaccggg ctcactgggt caccgtgaaa
    16801 ccttcgcgga tatcggccag actctggcaa aatattttgg tacttctgat atggaatatg
    16861 gcaaagccat gttctgataa tgggtgcggc ttgatgccct caccccggcc ctctccctca
    16921 gggagagggt gaaaacatgt agataaggat aaaacaatgg ctaccccaca cattaatgca
    16981 gaaatgggcg atttcgctga cgtagttttg atgccaggcg acccgctgcg tgcaaagtat
    17041 attgctgaaa ctttccttga agatgcccgt gaagtgaaca acgttcgcgg tatgctgggc
    17101 ttcaccggta cttataaagg ccgcaaaatt tccgtaatgg gtcacggtat gggtatcccg
    17161 tcctgctcca tctacaccaa agaactgatc accgatttcg gcgtgaagaa aattatccgc
    17221 gtgggttcct gtggcgcagt tctgccgcac gtaaaactgc gcgacgtcgt tatcggtatg
    17281 ggtgcctgca ccgattccaa agttaaccgc atccgtttta aagatcatga ctttgccgct
    17341 atcgctgact ttgacatggt gcgtaacgcg gtagacgcgg ctaaagcact gggcattgat
    17401 gctcgcgttg gtaacctgtt ctccgctgac ctgttctact ctccggacgg cgaaatgttc
    17461 gacgtgatgg aaaaatacgg catcctcggc gtggaaatgg aagcggctgg tatctacggc
    17521 gtcgctgcag aatttggcgc gaaagccctg accatctgta ccgtgtctga ccacatccgc
    17581 actcacgagc agaccactgc cgctgagcgt cagaccacct tcaacgacat gatcaaaatc
    17641 gcactggaat ccgttctgct gggcgataaa gagtaattgt gtttcgctga aaggcgattg
    17701 tcgtgtgaag ccggagtggg agactgctcc ggctttttag tatctattca tcaatagcaa
    17761 ctgatttata gatttactat tatttttcat atggatagaa aataaaatct attcaagtat
    17821 ctattcacga atctattcat ttatgttcag ccgtcatcca ggaagggcat atcagcaact
    17881 ctggtggaca accataaatt tctgccattt tgcttttgat gtttgagcta aaccgttccg
    17941 tcttttcgat ctattttaga gaattaatcg agatacctaa cattttactt acttcagcaa
    18001 gcgataattt atgcctgata cgcacggcgg ctagccagga aatacgctgg cttaacatta
    18061 aattgataat ctcccgctcc tggttttcca gatcgatcat attcccttcc tttggtattt
    18121 taatcagcga gttaccttac cgcccccgct atccacgtcg acaactcccg tagctctttt
    18181 tcctggtccg ggaagtcaac caacagcgct tcgcactctt gttgcagcat atccgcgcgg
    18241 tacaggcagc cttgcagtcg cccggcgagg gcttccagcg gcgcggggtt gaggctgtcg
    18301 gtaaacacct gggcgcgggt gatatggcct ttttcaacgt cgaaatgcag ttccacgccg
    18361 ccccagctaa agcgttcatc cagcagatgc gagaatgccg gagcctgacc gaagttccat
    18421 tcccagctac tctgacgggc aaaggtttcg gcgaagtttg gcaagtctgg cgttttgtcc
    18481 ggggagatga tttccgcttc tacacgctcg ccataatggg cgaaaaaggc cttggttatg
    18541 gcctcgcaaa cctgctcatg ggtgatcccc ggcagcagct cggtgaggtt ggtcacgcgg
    18601 gaacgcactg aggtaatgcc tttcgccgcc agtttctttt tatccggatt gagatagttt
    18661 gccaggcggc taaggtcggc attgagcagc aaggtgccgt ggtggaagcc acgatctttg
    18721 gtttcgcgat aggccgatcc tgagactttg cggtcgcctt cgactgtttt caccaccaga
    18781 tcgttacgtc cggacgcttc ggcgctgacg ccgagcgcgt tcagcgcatt gagcacaatc
    18841 gacgtagaga tggttttatc gtactccggc ttgccagcca taaaggtaaa gcaggtattg
    18901 ccgagatcgt ggaacaccgc gccgccaccg ctactgcgcc gtgccaggcg gacgttatct
    18961 tcttccatcc gccgggtatt acactctttc cacgggttct gcgcgcgacc aatcactacc
    19021 gtgtcggcat tgcgccagag aaacagaacg cgctgcgtgg cgggcatctg gcgaaaaata
    19081 cactcctcca ccgccaggtt aaaccacggg tcgtaagagt cagagatgag caggcgtaat
    19141 gtggacataa cgatttcctt tcttgtagta atgggtcact cttttttctc gctttcttcc
    19201 tcttccggca ccggtttgct ggcggttaac aggaagggcg attgctgcca gcgggtacgt
    19261 ttaccctgta gcagcgtgcg ggtcagcact acgccgattg ccagtgaaag cagcaacatc
    19321 aggcgtaaaa tgttagtggt gttatccacc tgttgggctt cggtggcgag cgtatgggtg
    19381 tcgagcgtca ggcgcagata gccgagcggt ccgtttttcc cggcaattgg ttcgacaatc
    19441 tgctggttaa aatagccgcc cgctttttta ccgtcgagcg ccagccggtc gcgcacttcg
    19501 acgctttcgc cagaacgtgc gataagatcg ccttgttcgt catacacacc cgcgtcgagg
    19561 atacggcttt catccgttaa ctgatcgagg atcgcctgaa tgcgtttttc atccggtgag
    19621 tcggtacgca tcagcggtgc aacgttcagc gttacctgac gcgccagggt gcgggccagt
    19681 tcttccagct gtggattacg ttgtcgctgg tggttttgac taaaccatga cgctccctgc
    19741 atcagcgcca ctaacaaggc aagacagaac aggacaatca ctgcccgatg cagccggaat
    19801 ttcagttttg tgcgagccat cttccaccct ttgaaaattt gagacttaat gttgccagaa
    19861 gcaatggata caaggtagcc tcatgcgtta ttttccctgc ttcgaacgat tttacaggag
    19921 ccttaatgcc taacattacc tggtgcgacc tgcctgaaga tgtctcttta tggcctggtc
    19981 tgcctctttc attaagtggt gatgaagtga tgccactgga ttaccacgca ggtcgtagcg
    20041 gctggctgct gtatggtcgt gggctggata aacagcgtct gacccaatac cagagcaaac
    20101 tgggtgcggc gatggtgatt gttgccgcct ggtgcgtgga agattatcag gtgattcgtc
    20161 tggcaggttc actcaccgca cgggctacgc gcctggccca tgaagcgcaa ctggatgtcg
    20221 ccccgctggg gaaaatcccg cacctgcgca cgccgggttt gctggtgatg gacatggatt
    20281 ccaccgccat ccagattgaa tgtattgatg aaattgccaa actggccgga acgggcgaga
    20341 tggtggcgga agtaaccgaa cgggcgatgc gcggcgaact cgattttacc gccagcctgc
    20401 gcagccgtgt ggcgacgctg aaaggcgctg acgccaatat tctgcaacag gtgcgtgaaa
    20461 atctgccgct gatgccaggc ttaacgcaac tggtgctcaa gctggaaacg ctgggctgga
    20521 aagtggcgat tgcctccggc ggctttactt tctttgctga atatctgcgc gacaagctgc
    20581 gcctgaccgc cgtggtagcc aatgaactgg agatcatgga cggtaaattt accggcaatg
    20641 tgatcggcga catcgtagac gcgcagtaca aagcgaaaac tttgactcgc ctcgcgcagg
    20701 agtatgaaat cccgctggcg cagaccgtgg cgattggcga tggagctaat gacctgccga
    20761 tgatcaaagc agcagggctg gggattgcct accatgccaa gccaaaagtg aatgaaaagg
    20821 cggaagtcac catccgtcac gctgacctga tgggggtatt ctgcatcctc tcaggcagcc
    20881 tgaatcagaa gtaattgctc gcccgccatc ctgcgggcgg cacagcatta acgaggtaca
    20941 ccgtggcaaa agctccaaaa cgcgcctttg tttgtaatga atgcggggcc gattatccgc
    21001 gctggcaggg gcagtgcagt gcctgtcatg cctggaacac catcaccgag gtgcgtcttg
    21061 ctgcgtcgcc aacggtggcg cgtaacgagc gtctcagcgg ctatgccggt agcgccgggg
    21121 tggcaaaagt ccagaaactc tccgatatca gccttgaaga gctgccgcgt ttttccaccg
    21181 gatttaaaga gttcgaccgc gtactaggcg gcggcgtggt gccaggaagt gccattctga
    21241 ttggcggtaa ccctggtgcg gggaaatcca cgctgttgct gcaaacgctg tgcaaactgg
    21301 cccagcagat gaaaacgctg tatgtcactg gcgaagagtc gctgcaacag gtggcaatgc
    21361 gcgctcatcg ccttggcctg ccgactgaca atctcaatat gttgtcggaa accagcatcg
    21421 aacagatctg cctgattgcc gaagaagagc aaccgaaact gatggtaatt gactcgatcc
    21481 aggtgatgca tatggcggat gtacaatcat cgcctggtag cgtggcgcag gtgcgtgaaa
    21541 cggcggctta tctgacgcgc ttcgccaaaa cgcgcggtgt ggcgattgtc atggttgggc
    21601 acgtaaccaa agatggctcg ctggctggcc ctaaagtgct ggaacactgt atcgactgtt
    21661 cggtgcttct ggatggtgat gccgactccc gttttcgcac cttgcgcagc cataaaaacc
    21721 gcttcggcgc ggtgaatgag ctgggcgtct tcgcgatgac cgaacagggg ctgcgtgaag
    21781 tcagcaaccc ttcggcaatt ttcttaagtc gcggagatga agtgacctcc ggcagctccg
    21841 tgatggtggt gtgggaagga acgcgtccgt tgctggtgga gattcaggcg ctggtcgatc
    21901 actcgatgat ggcgaatccg cgccgcgtgg cagtggggct ggaacaaaac cgtctggcaa
    21961 tcctgctggc tgtcttgcac cgtcacggtg gtctgcaaat ggccgatcag gatgtatttg
    22021 tgaacgtggt cggcggcgtg aaggtaaccg aaaccagtgc cgatttagcg ttactgctgg
    22081 cgatggtttc cagcctgcgt gacagaccgc tgccacagga tctggtggtg tttggtgaag
    22141 tcgggctggc aggggagatc cgcccggtgc ccagcggtca ggaacgaatt tcagaagcgg
    22201 cgaaacacgg ttttcgccgg gcgattgttc cggcggctaa cgtaccgaaa aaagcgccgg
    22261 aagggatgca gatttttggc gttaaaaaac tctccgacgc gcttagcgtg ttcgacgact
    22321 tataatgaga gatacggagg gagatatgtc gtcatttgat tacctgaaaa ctgccatcaa
    22381 gcaacagggc tgcacgctac agcaggtagc tgatgccagc ggtatgacca aagggtattt
    22441 aagccagtta ctgaatgcca aaattaaaag ccccagcgcg caaaagctgg aggcgttgca
    22501 ccgttttttg gggcttgaat ttccccgaca gaagaaaacc attggtgtcg tattcggtaa
    22561 gttctacccg ctgcataccg gacatatcta ccttatccag cgtgcctgta gccaggttga
    22621 cgagctgcat atcattatgg gttttgacga tacccgcgac cgcgcgttgt ttgaagacag
    22681 cgccatgtcg cagcagccca cggtgccgga tcgtctgcgc tggttattac aaacttttaa
    22741 atatcagaaa aatattcgca ttcatgcttt caacgaagag ggcatggagc cgtatccgca
    22801 cggctgggat gtgtggagca acggcatcaa aaagtttatg gctgaaaagg ggattcagcc
    22861 ggacctgatc tacacctcgg aagaagccga tgcgccacag tatatggaac atctggggat
    22921 cgatacggtg ctggtcgatc cgaaacgtac ctttatgagt atcagcggtg cgcagatccg
    22981 cgaaaacccg ttccgctact gggaatatat tcctacggaa gtgaagccgt tctttgtacg
    23041 taccgtggcg atccttggtg gcgagtcgag cggtaaatcc accctggtaa acaaacttgc
    23101 caatatcttc aataccacca gtgcgtggga atatggtcgc gattatgtct tttcacacct
    23161 cggcggtgat gagatcgcat tgcagtattc cgattacgat aaaatcgcgc tgggccacgc
    23221 acaatacatt gattttgcag tgaaatatgc caataaagtg gcgtttattg acactgattt
    23281 tgtcactacc caggcgttct gcaaaaagta cgaagggcgt gagcatccgt tcgtacaggc
    23341 gttgattgat gaataccgtt tcgatctggt gatcctgctg gagaacaaca cgccgtgggt
    23401 ggcggatggt ttacgcagcc tcggcagttc ggtggatcgc aaagagttcc agaacttgct
    23461 ggtggagatg ctggaagaga acaatatcga attcgtgcgg gttgaagagg acgattatga
    23521 cagccgtttc ctgcgctgcg tggagctggt gcgggagatg atgggggagc agagataacc
    23581 gcgatgaaac ggctcaaagg cgaggtataa aataagtttt ttgatgagtt gaaaacctct
    23641 caggaagagg ctgtcgagat taaacaaggt ttaaaagaat cagcacgggt gacccgctac
    23701 gaatttgcgg atactaaggc tgttgtaggc cggaaaaggc gttcacgccg tatccggcat
    23761 tcggtgctca atgcctgatg cgacgcttac cgcgtcttat caggcctgga gattcacaac
    23821 gtattgaaat ttcttggttt gtaggccgga taaggcgttc acgccgcatc cggcatttta
    23881 cgcattactt cgcaatacgc ttgtacttga tacgcttcgg ctccagtgcg tctgcgccca
    23941 gcgtgcgttt cttgtactct tcgtactcgg taaagttacc ttcgaagaac tcaactttac
    24001 cttcatcctg gtaatccaga atgtgcgtgg cgatacggtc gaggaaccaa cggtcgtgcg
    24061 agataaccat cgcgcagccc gggaactcca gcagggcgtt ttccagcgcg cgcagggttt
    24121 cgatatccag gtcgttggtt ggttcgtcga gcagcagcat gttgccgcca acctgcagca
    24181 gcttcgccag atgcagacga ccgcgctcac caccggaaag ttcaccaacg cgtttaccct
    24241 gatcaacccc tttaaagtta aagcggccaa cgtaggcgcg gcttggcatc tcggtgttgc
    24301 cgatcttcat aatatccagc ccgccggaaa cttcttccca aacggttttg ctgttatcca
    24361 ttgagtcacg gaactgatca accgatgcca gtttcaccgt ttcacctaaa gtgatggtgc
    24421 cgctgtccgg ctgttcctga ccagagatca tacggaacag ggtcgattta cccgcgccgt
    24481 tcggaccgat gatcccgacg attgcccctt tcgggatcga gaagctcagg tcatcaatca
    24541 gcaggcgatc accgtaggat ttacgcaggt tgctgacttc cagcacttta tcgcccagac
    24601 gcggtccagg tggaataaac agttcgttgg tttcgttacg tttctgatat tcggtgctgt
    24661 tcagctcttc aaagcgtgcc agacgtgctt tacctttcga ctggcggcct ttagttccct
    24721 gacgtaccca ctccagctct ttctcgatcg acttacgacg cgccgcttct tgtgaagctt
    24781 cctgcgccag gcgctgatct ttctgctcca gccaggagga gtagttgcct tcccacggaa
    24841 taccttcacc gcggtcaagt tcgaggatcc agcctgcaac gttatcgagg aagtaacggt
    24901 cgtgggtaat cgccaccacg gtgccctcga agtcgtgcag gaagcgttcc agccaggcca
    24961 cggattcggc atccaggtgg ttggtcggtt cgtcgagcag cagcatgtct ggtttttcca
    25021 gcagcaggcg gcacaacgct acgcgacgac gctcaccacc ggagaggtta gcgattttcg
    25081 cgtcccaatc tggcagacgc agcgcatccg ccgcacgctc cagctgtacg ttcaggttat
    25141 gaccgtcgtg agcctgaatg atctcttcca gacggccttg ttcagcggcc agcttgtcaa
    25201 aatcggcatc cggatcggcg tacagcgcat acacttcatc caggcgtttc agggcgttaa
    25261 ccacttcaga aaccgcttct tcaatggact cacgcacggt gtgttccggg ttcagctgcg
    25321 gttcctgcgg caggtaacca atcttgatgt ctggctgcgg acgcgcttca ccttcgatgt
    25381 ctttatcaat gcccgccata atgcgcagca gggtagactt acccgcgccg ttcagaccca
    25441 ggacaccaat ttttgcccca gggaagaaac tcagagagat gtttttcaaa atatgacgtt
    25501 tcggcggaac aactttgccg acacgatgca tggtataaac gaattgagcc acgttggact
    25561 tcgcctctat gtttatcgtg atgatgagtt ttcaaaggcg aagtgtagcc tttttccccg
    25621 cctaatccca gttggtcgat caccttcgca gtaaaagtaa aaaagtgtcc gtaacgtggc
    25681 gtaaacggca atgactggtt agcataaatc tattacgcgg catgacgctg cattgatgta
    25741 tttacactta gaggatgcgc ttgtggaaaa agccaaacaa gttacctggc ggctgttggc
    25801 tgccggtgtc tgtctgctga cggtcagcag cgtggcgcga gccgactcac tggatgagca
    25861 gcgtagccgt tacgcgcaaa tcaagcaggc ctgggataat cgacaaatgg atgtggtcga
    25921 acaaatgatg cctggactga aggattatcc gctttatccc tacctggaat accgccagat
    25981 caccgatgat ctgatgaatc aaccggcggt gacggtcact aactttgttc gcgctaaccc
    26041 cacgcttcct cccgctcgca cgctgcaatc tcgtttcgtc aatgaactgg cgcggcgtga
    26101 agactggcgt ggcttgttag cctttagccc ggaaaagccc ggaactaccg aagcgcaatg
    26161 taattactac tatgcgaaat ggaacaccgg gcagagtgaa gaagcctggc aaggggcgaa
    26221 agagctgtgg ctaaccggca agagccagcc taacgcctgt gacaagttat ttagcgtctg
    26281 gcgtgcgtca ggtaaacaag atccgctggc gtatttagag cgtatccgtc tggcgatgaa
    26341 agcgggtaac actggcctgg taacagtgct ggcagggcag atgcctgccg attaccagac
    26401 tatcgcctcg gcaatcattt cactggcgaa caaccctaat acggtactga ccttcgcgcg
    26461 tacaaccgga gcgaccgatt ttacccgtca aatggcagcg gtggcgtttg ccagtgtggc
    26521 gcggcaggat gctgaaaatg cacggctgat gatcccgtcg cttgcccagg cgcagcagct
    26581 taatgaagat cagattcagg agctgcgcga tatcgtcgcc tggcgtttga tgggcaacga
    26641 tgtcaccgac gagcaggcga aatggcgcga tgacgccatt atgcgctcgc aatccacgtc
    26701 gttgattgaa cgccgtgtgc gaatggcact tggcaccggc gatcgtcgcg gcctgaatac
    26761 ctggctggcg cgtctgccga tggaagcaaa agagaaagat gaatggcgtt actggcaggc
    26821 ggatttattg ctggaacgcg gacgtgaagc tgaagcgaaa gagattttgc atcaactcat
    26881 gcaacagcgt ggtttctacc cgatggttgc agcacaacgc atcggagaag agtatgagct
    26941 gaagattgat aaagcgccgc agaatgttga cagcgccctg actcaggggc cggagatggc
    27001 gcgcgtgcgc gagttgatgt actggaatct cgataacacc gcgcgtagcg agtgggccaa
    27061 tctggtgaag agcaagtcaa aaacagagca ggctcaactg gctcggtatg ctttcaataa
    27121 ccaatggtgg gatcttagcg ttcaggcaac gatcgccggg aagctttggg atcatctgga
    27181 agagcgattc ccgctggctt acaacgatct tttcaaacgt tacaccagtg ggaaagagat
    27241 cccgcaaagc tatgcgatgg cgattgcccg tcaggagagc gcctggaatc cgaaagtgaa
    27301 atcaccggta ggggccagcg gcctgatgca gattatgcct ggtacagcga cccatacggt
    27361 gaagatgttc tctattcctg gttatagcag ccctgggcaa ttgctggatc cggaaacgaa
    27421 tatcaacatt ggcaccagtt acctgcaata tgtttatcag cagtttggca ataaccgtat
    27481 tttctcctca gcagcttata acgccggacc tggacgggtg cgaacctggc ttggcaacag
    27541 cgccgggcgt atcgacgctg tggcatttgt cgagagtatt ccgttctccg agacgcgcgg
    27601 ttatgtgaag aacgtgctgg cttatgacgc ttactaccgc tatttcatgg gggataaacc
    27661 gacgttgatg agcgccacgg aatggggacg tcgttactga tccgcacgtt tatgatatgc
    27721 tatcgtactc tttagcgagt acaaccgggg gaggcatttt gcttcccccg ctaacaatgg
    27781 cgacatatta tggcccaaca atcaccctat tcagcagcga tggcagaaca gcgtcaccag
    27841 gagtggttac gttttgtcga cctgcttaag aatgcctacc aaaacgatct ccatttaccg
    27901 ttgttaaacc tgatgctgac gccagatgag cgcgaagcgt tggggactcg cgtgcgtatt
    27961 gtcgaagagc tgttgcgtgg cgaaatgagt cagcgtgagt taaaaaatga actcggcgcg
    28021 ggcatcgcga cgattacgcg tggatctaac agcctgaaag ccgcgcccgt ggagctgcgc
    28081 cagtggctgg aagaggtgtt gctgaaaagc gattgatt
//
LOCUS       DAPGWD010000049        27822 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPGWD010000049 DAPGWD010000000
VERSION     DAPGWD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.049"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            83..745
                     /gene="acrS"
                     /locus_tag="RHX88_002043"
     CDS             83..745
                     /gene="acrS"
                     /locus_tag="RHX88_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDV2230732.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(748..927)
                     /locus_tag="RHX88_002044"
     CDS             complement(748..927)
                     /locus_tag="RHX88_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDV2230733.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1011..1895)
                     /gene="yhdJ"
                     /locus_tag="RHX88_002045"
     CDS             complement(1011..1895)
                     /gene="yhdJ"
                     /locus_tag="RHX88_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV2230734.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1981..2277)
                     /gene="fis"
                     /locus_tag="RHX88_002046"
     CDS             complement(1981..2277)
                     /gene="fis"
                     /locus_tag="RHX88_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDV2230735.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2303..3268)
                     /gene="dusB"
                     /locus_tag="RHX88_002047"
     CDS             complement(2303..3268)
                     /gene="dusB"
                     /locus_tag="RHX88_002047"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDV2230736.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3597..4478)
                     /gene="prmA"
                     /locus_tag="RHX88_002048"
     CDS             complement(3597..4478)
                     /gene="prmA"
                     /locus_tag="RHX88_002048"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDV2230737.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4490..5941)
                     /gene="panF"
                     /locus_tag="RHX88_002049"
     CDS             complement(4490..5941)
                     /gene="panF"
                     /locus_tag="RHX88_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDV2230738.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5931..6173)
                     /locus_tag="RHX88_002050"
     CDS             complement(5931..6173)
                     /locus_tag="RHX88_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDV2230739.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6282..7631)
                     /gene="accC"
                     /locus_tag="RHX88_002051"
     CDS             complement(6282..7631)
                     /gene="accC"
                     /locus_tag="RHX88_002051"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDV2230740.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7642..8112)
                     /gene="accB"
                     /locus_tag="RHX88_002052"
                     /gene_synonym="fabE"
     CDS             complement(7642..8112)
                     /gene="accB"
                     /locus_tag="RHX88_002052"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDV2230741.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9090..10064)
                     /locus_tag="RHX88_002053"
     CDS             complement(9090..10064)
                     /locus_tag="RHX88_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV2230742.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10216..12156
                     /gene="csrD"
                     /locus_tag="RHX88_002054"
     CDS             10216..12156
                     /gene="csrD"
                     /locus_tag="RHX88_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDV2230743.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12461..13504
                     /gene="mreB"
                     /locus_tag="RHX88_002055"
     CDS             12461..13504
                     /gene="mreB"
                     /locus_tag="RHX88_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDV2230744.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13570..14673
                     /gene="mreC"
                     /locus_tag="RHX88_002056"
     CDS             13570..14673
                     /gene="mreC"
                     /locus_tag="RHX88_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDV2230745.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14673..15161
                     /gene="mreD"
                     /locus_tag="RHX88_002057"
     CDS             14673..15161
                     /gene="mreD"
                     /locus_tag="RHX88_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDV2230746.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15170..15763
                     /gene="yhdE"
                     /locus_tag="RHX88_002058"
     CDS             15170..15763
                     /gene="yhdE"
                     /locus_tag="RHX88_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDV2230747.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVITDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15753..17222
                     /gene="rng"
                     /locus_tag="RHX88_002059"
     CDS             15753..17222
                     /gene="rng"
                     /locus_tag="RHX88_002059"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDV2230748.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17290..21090
                     /gene="yhdP"
                     /locus_tag="RHX88_002060"
     CDS             17290..21090
                     /gene="yhdP"
                     /locus_tag="RHX88_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDV2230749.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFDLTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21246..22691
                     /gene="tldD"
                     /locus_tag="RHX88_002061"
     CDS             21246..22691
                     /gene="tldD"
                     /locus_tag="RHX88_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDV2230750.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22819..23748)
                     /gene="aaeR"
                     /locus_tag="RHX88_002062"
     CDS             complement(22819..23748)
                     /gene="aaeR"
                     /locus_tag="RHX88_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDV2230751.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23931..24134
                     /locus_tag="RHX88_002063"
     CDS             23931..24134
                     /locus_tag="RHX88_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDV2230752.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24142..25074
                     /gene="aaeA"
                     /locus_tag="RHX88_002064"
     CDS             24142..25074
                     /gene="aaeA"
                     /locus_tag="RHX88_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDV2230753.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25080..27047
                     /gene="aaeB"
                     /locus_tag="RHX88_002065"
     CDS             25080..27047
                     /gene="aaeB"
                     /locus_tag="RHX88_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDV2230754.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27139..27411
                     /locus_tag="RHX88_002066"
     CDS             27139..27411
                     /locus_tag="RHX88_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDV2230755.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27467..27730)
                     /gene="yhcN"
                     /locus_tag="RHX88_002067"
     CDS             complement(27467..27730)
                     /gene="yhcN"
                     /locus_tag="RHX88_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDV2230756.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6681 a         6960 c         7398 g         6783 t
ORIGIN      
        1 ataaatacac attaggtgat agattaacct tcgctatttt ctcactctgt gtcgaatata
       61 tttatttcct gaataattaa tcatggcaaa aagaaccaaa gccgaagctc tgaagacccg
      121 gcaagaactg attgaaactg ccatcgccca gtttgcgcag catggcgtaa gcaagacgac
      181 gctcaacgac attgccgacg ccgctaacgt tacgcgtggc gctatctact ggcacttcga
      241 aaacaagact caactgttta atgagatgtg gttgcaacag ccttcattgc gggagttaat
      301 ccaggaacac ttgacggctg gattagagca tgacccgttt caacaattgc gtgaaaaatt
      361 gattgtcggc ttgcaatata ttgccaaaat tccccgccag caggcgttgc tgaaaatctt
      421 atatcacaaa tgtgaattta atgatgagat gctggccgag ggagtgatac gcgaaaagat
      481 gggcttcaat ccgcagactc tccgcgaagt attgcaggcg tgtcagcaac aaggttgtgt
      541 agcaaataac ctcgatttag atgttgtgat gattattatt gatggtgcct tcagcggaat
      601 tgttcaaaac tggttaatga atatggcggg ttatgatctt tataaacaag cacccgcgct
      661 ggtcgataac gtattaagaa tgttcatgcc agatgaaaac ataacgaaat taattcatca
      721 aacgaatgaa ttaagtgtca tgtaaattca cattctgtcc tgaaaattca gttcgtcaca
      781 gttaaccagt tttagtggat taactgagtt catattacgg catttgtctg tttctgttgt
      841 cagcagttca atccactgca tcagtaaagt atcaaacaga aaaacgaaga cagcgaaaac
      901 gacgagccac caatacttgc gaatcattac ctgagcctgt gcatgtttaa ccaagtctgg
      961 caggaaatat acacgaaatg aataatctga aaaccaggct tacacttcct ttactttgca
     1021 atgagatcgg ggtcaacttc gcttaaccgg cttcgttttg acaggttgcc ggtctttctt
     1081 tttttcactt tcgccagttc ttccgcagag taatgcgacg cgacatccag ccgtcgaagc
     1141 cccattttga tgtactcgct gttgatctca ataccaatga attttcgccc gctggagacg
     1201 gctacggcac cggtagtaaa gctaccggca aacgggtcga gaacgatatc gcctgggttg
     1261 gaagaggcga gaataatgcg tttcagtaag gcttccggtt tttgcgtcgg gtggttctca
     1321 tattcatcca tcaaataacg cacgcgcgga aaatcccaga cgttacccgg tactttttga
     1381 tgattgtatg gctgtggagg atttttgcga taatcgatca acgcgcgctg cgatccggtt
     1441 ttggcttcta ccagaatagc atcaccgttg aatgtgtagt tctttgcgtc tttcaccatc
     1501 atcaggatgg gttcgtacat ggagccgtag tgttttttcg cctgtactcc agaactgtca
     1561 tatgaccaga cgatgcgact tttgatggta aaaagcttgc ggcactggag atcgataaag
     1621 ggcatgtttt ccgtgctgtt catgatgtac atgctgccct gctttttcag aacgcggtgg
     1681 cactctgcaa tcacttcaaa cagccagtcg ataaacagat cttctttcca ggcttcgatc
     1741 agaccatcaa aatttttacc gatgttatat ggtgggtcgg caaagatcag atcgacactt
     1801 tcggcgggga tctttttaag ttcggcaagc gcatcaccgt gaataatggt cttagcttca
     1861 ttaccaaacc gggtcggttc acatcctgtt ctcatggtca ctccctttgt gacacctata
     1921 aaaaaggcgc ttccccatgc cgagtagcgc ctttttaatc aagcatttag ctaacctgaa
     1981 ttagttcatg ccgtattttt tcaatttttt acgcagcgta ccacggttga tgcccatcat
     2041 cagcgcagca cgggtctggt taccacgggt gtattgcatc accatgtcca acaggggctg
     2101 ttctacttca gccagtacca gctcatagag gtcattcaca tcctgaccat tcagttgagc
     2161 aaaatagttc ttcagtgcct gtttaaccga gtcacgcagg ggtttttggg ttacctgatc
     2221 ctgagagtta acggtagaaa cggtcagtac gtcagaattt acgcgttgtt cgaacatagt
     2281 tctgtcagct ctttatttct gtttacgcaa aattttcgaa gtatgcctcc aacgcctcca
     2341 gctgttcgct ggcatcctca atggcgttga atgtgcgccg aaactggtca tttggagcgt
     2401 gttcctggag ataccaggaa acgtgtttac gtgcaattcg gtaccctttt gccggaccat
     2461 aaaagtcatg cagttcccga acgtgcgcgc aaagcaagcg cttaacctct gccaaaggca
     2521 gcgggggcag caactcccca gtgtccagat aatgctggat ttcccgaaag atccagggtc
     2581 ttccctgagc tgcgcggcct atcatcaggg catccgcccc tgtatagtcg agcacagctc
     2641 tggctttaag cgggtcagta atgtcgccat tcgcgataac cggaatggaa actttctgct
     2701 taactgcccg aatactgtcg tactcagctt ctccattgaa caaacaggcg cgtgtacggc
     2761 catgaatggt cagagcctga atgccacagt cttcagccag ttgggcaatc tcttcgcagt
     2821 tacggtgttc cggtgcccag ccggtgcgaa tcttcagggt aacaggaacg tccactgcat
     2881 tgacgacctc ggtaaggatc gatttaacga catccgggta ctgcaagagg gctgaacctg
     2941 cgagcttgcg attcactttt ttagccgggc aacccatatt gatatcaata atctgggcac
     3001 cgctttccac gttaatacgt gctgcatctg ccatttcttt cggatcgcta ccagcaattt
     3061 gcacggtgcg aataccgggt tcatcaatgt gcaccatccg taaacgagat ttgtcgcttt
     3121 cccaaacctg tgggttagaa gacatcatct cggatactgt caatccggct cccatctcgt
     3181 agcacaacgt ccgaaaaggt ctgtctgtaa tgccagccat gggcgctgcg atcaggcgat
     3241 ttctgagctg atattgtccg atgcgcatga gttaagaaat gaccatactg tgactgcaag
     3301 gcggcgtata ttacgcattt tttgcacgag atgaaaggcc aaactttgac caatcctctg
     3361 agatggatca aagaattgca tttaaaatga gcgtggtgcg ataattactc ataaaaatca
     3421 tcatattaga aaatagtgac taaaatttac actcaaagaa atttgagtaa gttctcaatt
     3481 tttctttatg aatgaaaatt ttggcacgca aatttgcgta aataatcggc aaatttacgt
     3541 gccttttgtg agcttgctcg cacttcgccc cgcgtcaccc tacggcgatg cgaaggttaa
     3601 ttcttacgac cggtaatacg gcaccactct tctttttcca cgaccgggtc cagtgcgaag
     3661 ctgtcggcat aagcttcaca aacgctctct gcctggcttg ccagaatacc ggaaaggccc
     3721 agcaaaccgc ctgaaaccgg caggacgctg attaacggtg ccagttcacg taatgggcct
     3781 gcaaggatgt tagcgaccac cacgtcggct ttcatttctt ctggctgatc tttcggtaag
     3841 tagagttcca gacggtcaga aacgccatta cgttcggcgt tgtcgcggct ggcctgaatc
     3901 gcctgcggat cgatatcaat accaatggct tttgctgcac ccagtttcag tgccgcgatc
     3961 gccagaatgc cggaaccaca gccaaagtcg atgactgttt taccggttaa atcgaggctg
     4021 tcgagccatt gcaggcacag agaggtggtt ggatgggtac ccgtaccaaa cgccagccct
     4081 ggatctaaca tcacgttgac ggcgttttcg tccggcacat cacgccagct agggcagatc
     4141 cacagtcgtt caccaaagcg catcgggtgg aaattatcca tccattcgcg ctcccagtct
     4201 ttatcttcta gttgttcgat tttatgcgcg aagcctgcgc cgagcagcgg atggttttcc
     4261 agaatcgcca ccacgtcgtt catatcggtt tcagcgtcaa acagaccaat cacatcggtg
     4321 tcgccccaca ggcgcgtttc gcccggcagc ggctcaaata ctggcgtatc gtgggtatcc
     4381 tgaaaagtga tagaaacggc acccgcttcc atcagcgcat cgctaagatc ttccgcgttc
     4441 gcgccggtgg tgttcagttt cagttggatc caaggcatgg caaaactctt tatttatcag
     4501 tagtcaaaac ggtagcttgc gggacggatg taccgaaacg gtttccgacc aggaaagcca
     4561 gcaaacttag tagtaacgag ggcacgatag ggtggaagcc caggtactga atattcagcg
     4621 tcgcgagtac ggcatacagc acgccgccaa cgatcatcgc acttagcgcg cctttggcgt
     4681 tggcgcgttc ccagtaaaga cccagcacca gcggccacag gaaaacggct tccagcccac
     4741 cgaaggccag caaattcagc cagatgatca tttctggcgg cttccaggcg gcaagcagca
     4801 gcaacgcgcc gagaactaac gtaattaccg ccgacatccg cttcagacgc gtctcgtttt
     4861 gcatttgatc cggacggata ttcagataga gatctttaat gatcgtagcg gaactttgca
     4921 gcaactgggc attgatcgtc gacatgatcg cagccatagg tgcagccagg aagattccgg
     4981 cagcaaacgg tggcagcact tttaccatta acgtcgggat caccagatcc ggtacggtga
     5041 gatcggggat cactgcacga cctaacgctc cggccaggtg cataccgaac atcaggattg
     5101 cgactacaat cgtaccgatg atgatccccc ggtgtactgc tttgctgtct ttataagaga
     5161 tacagcgcac cgcagtatgc ggcaggccaa tcacgccaaa gcacaccagt acccagaacg
     5221 acgtcataaa ggcaggcgac agaatatcgt cagcgccttg tggcgtaacc agttgcggat
     5281 cgatggtttg caaggtctgt actgcgttgc ttaagccgcc agcggcatgt accacgccaa
     5341 taagcagcac aacggtgcca atcagcatca caagcccttg catggtgtcg ttcagcacgc
     5401 tggcgcgaaa gccaccaaag gcggtatata acgcaatgct gataccaaaa atcagcagcc
     5461 cggtttcata aggaataccc gccgcggttt ccagcaggcg cgcaccgccg ataaactgca
     5521 cggtcatcgc accaacgaac gcaaccagca aactcaaact cgccagccac accagaagac
     5581 gactctggta gcgggcaaac agcatatcgt tcagcgtcac tgcattgtag cggcgcgcaa
     5641 gaatcgcaaa cttcttgccg agaataccga gtgaaagcca gactgcagga agctgaatca
     5701 tcgccagcaa tacccagccc agcccgtatt tataagcagc tcctggcccg ccgataaacg
     5761 aactggcact gatataggtc gcggtgagcg tcatcgccag cacaataccg cccatagagc
     5821 ggctgccgag gaaatactca ttaaggaagg tgccggtgct ccgtttacgc atcgcataaa
     5881 ccgagatacc gaacaccacc accagatagg cgaccagcgg tagaattact tcaagctgca
     5941 tcgtcatcct ccagtgggat atcgcgatag ataaatttca ccatcgccca gcacagtcca
     6001 ataaacagta gcggcgtcag gatgcaggcc atctcaaacc agcgcggaaa gccggtaaaa
     6061 ccgggggcaa cgccaggtaa gtaagcggct actaaccaaa ctgccagata caaaagggtc
     6121 agccccagcg cccagcgcgc ctctttatgg gcctgaacaa aacgagtgtc cattttttgt
     6181 ccctgatggg tgaagaaagc gaggattgta ccttatgggg gttgtcgatc cccagtaata
     6241 aaaaaggccg gaaaatccgg ccttttgacg cttttgcagt cttatttttc ctgaagaccg
     6301 agttttttct ccagatagtg gatgttagtg ccaccatgct ggaagttctc gtcattcatg
     6361 atgcggatct gcagatcaac gttggttttg ataccgtcga tgatcagctc ctgcagcgca
     6421 ttcttcatgc gagcaatcgc cacgtcacgg ttttcaccgt agcaaatcag cttaccgatc
     6481 attgagtcat agtacggcgg tacggtgtag cccgcgtaga tatgagactc ccaacgcact
     6541 ccaaaaccgc caggtgcgtg gaaacgggtg attttgcctg ggcttggcag gaaggtgttc
     6601 ggatcttcgg cgttgatacg acattccacc gcatggccgc gaacgtgaac ttcttcttgc
     6661 ttgatcgaca gcggttgacc ggcagcgata cgcagctgtt ctttgatcag gtcaacgccg
     6721 gtgatcattt ctgtaaccgg gtgttctacc tgaatacggg tgttcatttc gatgaaatag
     6781 aactcgccgt tttcgaacag gaactcgaaa gtacctgcac cgcgatagcc gatatcaaca
     6841 cacgctttag cacaacgttc gccgatgtag cgacgcagtt ccggggtaat gcccggtgct
     6901 ggtgcttctt cgaccacttt ctggtggcgg cgctgcatgg agcagtcacg ttccgccaga
     6961 tagatagcgt tgccctgacc gtcagccagt acctgaatct cgacgtggcg aggattttcc
     7021 aggtatttct ccatgtaaac catatcgttg ctgaaagcag ctttcgcttc cgcacgggtc
     7081 atggagatgg attgtgccag ttcagcgtcg ccgcgcacta cgcgcatacc gcgaccaccg
     7141 ccgccgccgg aggctttgat aatcaccgga taaccaatgc gtttagcaat ggcacggttt
     7201 ttatccatat cgtcgcccag cgggccgtca gaacccggta cgcaaggaac gcccgctttt
     7261 ttcatcgccg cgattgcgga tactttgtcg cccatcaggc gaatggtttc tgctttcggg
     7321 ccgatgaaga taaagccgga gcgttcaacc tgctcggcaa agttggcgtt ctcggagagg
     7381 aagccgtaac ccggatggat tgctactgcg ccggtgattt cagcggcgct gatgattgcc
     7441 gggatgttca gataactttt tactgacgga gcagggccga tacagaccgt ttcatctgcc
     7501 agtaatacgt gttttagatc gcgatccgcg ctggagtgca cagcgacagt cttgatgccc
     7561 agttctttac aggcacgaag aatacgcaat gcaatctcgc cgcggttggc aataacaatt
     7621 ttatccagca tgttcgcctc gttactcgat gacgaccagc ggctcgtcaa attctaccgg
     7681 ttgtccactt tcgaccagaa ttgctttcac ggtaccggat ttgtccgctt cgatctggtt
     7741 catcattttc atggcttcaa cgatgcacag ggtatcgccc acgttgactt tctgacccac
     7801 ttcgatgaac gcttttgcgt ccgggcttgg ggtgcggtag aaagtaccaa ccatcgggga
     7861 acgtacgatg tgaccactga tttccgctgc tgctggcgct tccatggaag gaacggtcgc
     7921 cggagcggct gcgttagatt gagctggctg ctgcatcatt ggtgcagcgt aagcttgttg
     7981 catcacaggg aaacttgcgg caggagctgc acggctaatg cgtactgact cttcgccttc
     8041 agaaatttcc agttcggaga tgcctgattc ttcaaccagc tcgatcagtt ttttaatctt
     8101 acgaatatcc atgagtgggt tccgtactct ttgtttagtg tgattgtgac aggcgtttca
     8161 ccgccgtctg taaagcgtat gccccgacag ctgtatgcat agcgataaat tccagcaggc
     8221 cgggacgccg gtctattttg ccccagagtg tcaatgttag acttgacgga cattgtgcag
     8281 cgatctgctg ctatcctccg gcaaaaaaca aaatatacct tcgttgcgcc aatgtcacct
     8341 tttccgcagc gcaaaaactg cgtcagggag gacgaggtcg cacattataa cgatttcgta
     8401 gcaattggca gctaaatact ggtcttatca gggaagataa tcaacagcta acatgtaaat
     8461 aaccttcaac accgtgtaat ttgcaacaag ccgcacaatt cacgaaatta gcgccatcat
     8521 cgacggaact tcttataacg taaggtgaaa agctgtaaag ccagccctgc gaagatgagg
     8581 ggctgcaatg agataatctt cacagaccac aaataatgta gggatgccag gatcgcgaca
     8641 agatagacga cattacgcag cggttgctgg ggattaccca attttcactg cattgtctgg
     8701 atagatgtta atgctaaagc acgcaaaatt aaccgattga caatgctcag cattaaatag
     8761 gttcgagtga ttaatttgta gtcaaataaa atcagattat tcaccccatg gtaccagaaa
     8821 tcagtaacag gttaagcgta gtgtcgccca ggcaaagcac cataaaccta acaggcgacg
     8881 gaggcatatc aataatggct gtttagagga gtatcaatgc gcatcaattt gtggtcagtc
     8941 gaaatcatga ctttccagtt tggatgatgt tgtacaactt cctgaccaag gtgttcgacg
     9001 aaagctgtcc ggctaaccgc ttttcccgtt aaccgattga ataatgatgt caaaatgagt
     9061 tccctctctt attattcctt gctaaatggt tagttaacct tcaccagcgt gcgaccctgg
     9121 atctggttat taatgatggc ctcggcaaag ttcggtgcct ctgacagaga tatctctttt
     9181 gccgcctggg tatagaatga ttccggtaaa tcggcgacca gtcgctgcca ggcttgtgcg
     9241 cggcgttctg gtggcgtcat tactgaatcc accccttgca aacggacatt acgcagaata
     9301 aatggcatga ccgtggttgg cagagtaaaa ccacccgcca gaccacaggc agccacgcag
     9361 ccgccgtaat tcatttgcgc cagcactttt gccagcactt tgtcgccaac agtgtcaatt
     9421 gccccagccc agacttgttt ttccagagga cgggattcgg caaactcatc acgagggaga
     9481 atacgactgg cacctaaact tttcagatat tcatgggtac tttcgcgacc ggaaacggca
     9541 acgacctgat aacccaactt atgcagcagc gccacggcgg tactgccgac gccaccactg
     9601 gcacccgtca cgacaatctc cccgtcctgc gggcgaacac cggcatcttc cagcgccatc
     9661 acacacagca tggcggtaaa accggcagta ccgataatca ttgctttacg cgcgtccagc
     9721 ccttgcggca tggcaaccag ccagtcacct ttcactcgcg cctgctccgc cagcccaccc
     9781 cagtggtttt caccaacgcc ccagccagtg agtaacacct cctgaccggc atgaaaacgc
     9841 ggatcttcgc tggtgcgtac agttccggca aaatcgatcc caggaatcat cggaaaatta
     9901 cggatgattt ttcccttacc ggtaatcgcc agcgcatctt tatagttcag gctcgaccag
     9961 tgaacatcga ccgtgacatc gccctccggc aggcgacttt cgtccagagt ctgtactgat
    10021 gcgagagttt tgccgtcctg ctgttctaaa agtaacgcct gcataagtgg tcctcatgtg
    10081 catgatggat tggaaaataa tttctgaaga ctatactcgc taatggaaaa gcaaagccga
    10141 tgaagcgcaa taaattgcga gataaatctg atttgctagt atgcccgctt cctcactatc
    10201 ggagttaaca caaggatgag attaacgacg aaattttcgg cctttgttac gctgctcacc
    10261 gggttaacaa tttttgtgac tttgctgggc tgttcgctaa gtttctacaa cgccattcag
    10321 tataagttta gtcatcgcgt tcaggcggtg gcgacggcga ttgataccca ccttgtgtcg
    10381 aatgacttca gcgcattaag gccacaaatt accgaattaa tggtgtcagc agatatcgtt
    10441 cgtgtagacc tgctgcatgg tgataaacag gtttataccc tggccagaaa tggtagttat
    10501 cgtccggttg gcaccagcga tctgtttcgt gaactgagcg ttccgttgat aaagcatccg
    10561 gggatgtcgt tgcgtctggt ttatcaggat ccgatgggca actatttcca ttcgttgatg
    10621 accaccgcgc cgctcacggg ggcgattggc tttatcattc ttatgctctt tctggcggta
    10681 cgctggttac aacggcaact tgccgggcaa gaattgctgg aaacccgggc tactcgtatc
    10741 ttaaacggtg agcgtggctc taatgtgttg ggaaccatct atgaatggcc gcccagaacc
    10801 agcagtgcgc tggatacgct gcttcgtgaa attcagaacg cacgcgaaca acacagccgt
    10861 cttgatacgc tgatccgctc ttatgccgca caggacgtga aaaccggcct caataaccga
    10921 ctctttttcg ataatcagtt agcaacgtta ctggaagatc aggagaaagt aggtacccac
    10981 gggatcgtga tgatgattcg tctgccggat ttcaatatgt tgagcgatac ctgggggcac
    11041 agccaggttg aagaacagtt cttcactctg acgaatctgc tgtcgacatt tatgatgcgc
    11101 taccctggcg cactgctggc gcgttaccac cgcagtgatt ttgctgcgct gttaccgcac
    11161 cggacgttaa aagaggcaga gagcatcgcc ggtcagttaa tcaaagccgt cgataccttg
    11221 ccgaacaata aaatgctcga tcgcgacgat atgatccaca ttggtatctg cgcctggcgt
    11281 agtggtcagg ataccgagca ggtaatggaa catgcagagt ctgccacgcg taatgcggga
    11341 ttgcagggcg gcaatagctg ggctatttac gatgactcgt tgcctgaaaa aggacgcggt
    11401 aatgttcgct ggcgtacgct tatcgagcaa atgctcagtc gcggcggccc gcgcctttat
    11461 caaaaaccgg cggttactcg cgaaggtcag gttcatcatc gcgaactcat gtgccgcatc
    11521 tttgatggta atgaagaggt tagctcggcg gagtatatgc cgatggtctt gcagtttggc
    11581 ttatcggaag agtatgaccg tctgcaaatc agccgtctta ttccactatt gcgttactgg
    11641 ccagaggaaa atctggcgat tcaggttacc gttgagtcgc tgattcgccc gcgttttcag
    11701 cgttggctgc gcgatacgtt aatgcaatgt gaaaaatcac aacgaaaacg cataattatt
    11761 gaacttgcag aggccgatgt aggtcaacat atcagtcgtt tacaacctgt tattcgttta
    11821 gtgaatgctt taggggtacg ggtagccgtc aaccaggctg gtttgacgct ggtaagcacc
    11881 agttggatca aagaacttaa tgttgagtta ctcaagctcc atccggggct ggtcagaaac
    11941 attgagaagc gaacggagaa ccagctgctg gttcaaagcc tggtggaagc ctgctcaggg
    12001 accagcaccc aggtttacgc caccggtgtg cgttcgcgaa gcgagtggca gaccctgatt
    12061 cagcgcggtg ttacaggcgg gcaaggggat tttttcgcgt cctcacagcc acttgatact
    12121 aacgtgaaaa aatattcaca aagatactcg gtttaacctg ccgtttaatc cgttttcacg
    12181 tagaataacg cgcgctgcgt ctcatgggag tgtgcttgtc tgctcgccag attgttgcag
    12241 cacatatgca gatgaatgac cttacgcggt tgcaaacagg cgaggaatgc tgctgatgca
    12301 ttaagccttt ctggactcag gcagagattt gtaacaaagg aaacgaactg cactaatttt
    12361 caccgtagca gatgattttt gcgccttgtc gctgctgcgt gtggttggta aagtaagcgg
    12421 attttctttt ccgccccagc tttcaggatt atcccttagt atgttgaaaa aatttcgtgg
    12481 catgttttcc aatgacttgt ccattgacct gggtactgcg aataccctca tttatgtaaa
    12541 aggacaaggc atcgtattga atgagccttc cgtggtggcc attcgtcagg atcgtgccgg
    12601 ttcaccgaaa agcgtagctg cagtaggtca tgacgcgaag cagatgctgg gccgtacgcc
    12661 gggcaatatt gctgccattc gcccaatgaa agacggcgtt atcgccgact tcttcgtgac
    12721 tgaaaaaatg ctccagcact tcatcaaaca agtgcacagc aacagcttta tgcgtccaag
    12781 cccgcgcgtt ctggtttgtg taccggttgg cgcgacccag gttgaacgcc gcgcaattcg
    12841 tgaatccgcg cagggcgctg gtgcccgtga agtcttcctg attgaagaac cgatggctgc
    12901 cgcaattggt gctggcctgc cggtttctga agcgaccggt tctatggtgg ttgatatcgg
    12961 tggtggtacc actgaagttg ctgttatctc cttgaacggc gtggtttact cctcttccgt
    13021 acgcattggt ggtgaccgtt tcgacgaagc tatcatcaac tatgtgcgtc gtaattacgg
    13081 ttctctgatc ggtgaagcca ccgcagaacg tatcaagcac gaaatcggtt cggcttatcc
    13141 gggcgatgaa gtccgtgaaa tcgaagttcg tggccgtaac ctggcagaag gtgttccacg
    13201 cggttttacc ctgaactcca acgaaatcct cgaagcactg caggaaccgc tgaccggtat
    13261 tgtgagcgcg gtaatggttg cactggaaca gtgcccgccg gaactggctt ccgacatctc
    13321 cgagcgcggc atggtactca ccggtggtgg cgcactgctg cgtaaccttg accgtttgtt
    13381 aatggaagaa accggcattc cagtcgttgt tgctgaagac ccgctgacct gtgtggcgcg
    13441 tggtggcggc aaagcgctgg aaatgatcga catgcacggc ggcgacctgt tcagcgaaga
    13501 gtaatcggat gcaggcaggg gaagcgtctg tttaccctgc ctggtctgat acgagaatac
    13561 gcataactta tgaagccaat ttttagccgt ggcccgtcgc tacagattcg ccttattctg
    13621 gcggtgctgg tggcgctcgg cattattatt gccgacagcc gcctggggac gttcagtcaa
    13681 atccgtactt atatggatac cgccgtcagt cctttctact ttgtttccaa tgctcctcgt
    13741 gaattgctgg atggcgtatc gcagacgctg gcctcgcgtg accaactaga acttgaaaac
    13801 cgggcgttac gtcaggaact gttgctgaaa aacagtgaac tgctgatgct tggacaatac
    13861 aaacaggaga acgcgcgtct gcgcgagctg ctgggttccc cgctgcgtca ggatgagcag
    13921 aaaatggtga ctcaggttat ctccacggtt aacgatcctt atagcgatca agttgttatc
    13981 gataaaggta gcgttaatgg cgtttatgaa ggccagccgg tcatcagcga caaaggtgtt
    14041 gttggtcagg tggttgccgt cgctaaactg accagtcgcg tgctgctgat ttgtgatgcg
    14101 acccacgcgc tgccaatcca ggtgctgcgc aacgatatcc gcgtaattgc agccggtaac
    14161 ggttgtacgg atgatttgca gcttgagcat ctgccagcga atacggatat tcgtgttggt
    14221 gatgtgctgg tgacctccgg tctgggcggt cgtttcccgg aaggctatcc ggtcgcggtt
    14281 gtctcttccg taaaactcga tacccagcgc gcttatactg tgattcaggc gcgtccgact
    14341 gcagggctgc aacgtttgcg ttatctgctg ctgctgtggg gggcagatcg taacggcgct
    14401 aacccgatga cgccggaaga ggtgcatcgt gttgctaatg aacgtctgat gcagatgatg
    14461 ccgcaggtat tgccttcgcc agacgcgatg gggccaaagt tacctgaacc ggcaacgggg
    14521 atcgctcagc cgactcctca gcaaccgacg acaggaaatg cagctactgc gcctgctgcg
    14581 ccgacacagc ctgctgctaa ccgatctcca caaagggcta cgccgccgca aagtggtgct
    14641 caaccgcctg cgcgtgcgcc gggagggcaa tagtggcgag ctatcgtagc cagggacgct
    14701 gggtaatctg gctctctttc ctcattgcgc tgttgctgca aatcatgccc tggccggaca
    14761 acctgattgt tttccggcca aactgggtgt tactcatctt gttgtattgg atcctggcct
    14821 tgccccatcg cgtaaatgtg ggcacaggtt ttgtgatggg tgccatactg gatctgatca
    14881 gcggctcgac gcttggcgta cgcgtattgg cgatgagcat cattgcttac ctggtggcgc
    14941 tgaaatacca gcttttccgc aacctcgcat tatggcagca ggcgctggtc gtcatgttgc
    15001 tttcgctggt ggtggatatt attgttttct gggcagagtt tttagtgatt aacgtctctt
    15061 tcagaccgga agtgttctgg agtagtgtag tcaatggggt gctctggccg tggattttct
    15121 tgctgatgcg taaagtccgt cagcagtttg cagtgcaata aaggtttcta tgacttctct
    15181 gtatttagct tccggttctc cgcgtcgtca ggagttactt gcgcaacttg gcgtgacctt
    15241 tgaacgtatt gttacgggca ttgaggagca gcgtcagccg caggagagcg cgcagcagta
    15301 tgttgtgcgt ctggcgcgcg agaaagcacg ggcaggtgtc gcgcaaacgg cgaaggatct
    15361 cccggtgctg ggtgcggata ctatcgttat cctgaacgga gaagtgctgg agaaaccgcg
    15421 tgacgcagag catgcggcgc agatgttgcg caaattatcg ggtcagaccc atcaggtgat
    15481 gacggcagtg gcgttggccg acagccagca cattctcgat tgcctggtga tcaccgatgt
    15541 gactttcaga acgttaacag acgaagacat cgcgggctat gtcgccagcg gtgaaccgtt
    15601 agataaagca ggtgcatacg gtattcaggg gctgggtggc tgttttgtca ggaagataaa
    15661 tggcagctat cacgccgtag tcggcttacc gctggttgaa acgtatgaat tattaagtaa
    15721 ttttaacgca ctgcgtgaga aaagggataa acatgacggc tgaattgtta gtaaacgtaa
    15781 cgccttcgga aacgcgagtg gcgtatattg atggcggtat tctgcaggaa attcatattg
    15841 aacgtgaggc gcgacgcgga atagtaggca atatctacaa gggtcgtgta agtcgtgtac
    15901 ttccgggtat gcaggcggct tttgtagata ttgggctgga taaagccgcg tttcttcatg
    15961 catctgacat catgccgcac accgaatgtg tggcgggtga agaacaaaag caattcacgg
    16021 tgcgcgacat ctcggaactg gttcgtcagg ggcaagatct gatggtgcag gtggtgaaag
    16081 atccgcttgg cactaagggg gcgcgcctga ccaccgatat cacgctgcct tctcgctatc
    16141 tggtctttat gccaggggct tctcacgttg gtgtttccca acgtattgaa agcgaatcag
    16201 aacgtgaacg cctgaaaaaa gtggtcgcgg agtattgcga cgagcagggc gggtttatca
    16261 tccgtaccgc agcggaaggg gttggcgagg ctgaactggc ctccgatgcc gcttatctga
    16321 aacgcgtctg gaccaaagtt atggagcgta aaaagcgccc gcagacccgt tatcagctgt
    16381 acggcgaact ggcgttggcg cagcgtgttc tgcgtgattt cgccgatgcc gaactggacc
    16441 gcattcgcgt tgactcacgc ctgacttacg aagcattact tgagttcacc tcggagtaca
    16501 ttcccgagat gaccagcaag ctggagcatt acaccggacg ccagccgatt ttcgatctct
    16561 ttgatgtcga aaacgaaatc cagcgagcgc tggaacgcaa agtagaactg aaatccggtg
    16621 gttatctgat tatcgaccag accgaagcga tgaccaccgt ggacatcaat accggagcgt
    16681 ttgtcggtca tcgcaatctg gacgacacca ttttcaatac caatattgaa gcgacgcagg
    16741 ctatcgctcg ccagttacgg ttgcgcaatc tgggcgggat tatcattatt gatttcatcg
    16801 atatgaataa tgaagatcac cgccgccgcg tgctgcattc gctggagcaa gcgttgagca
    16861 aagaccgggt gaaaaccagc gttaatggtt tttcggcgct ggggctggtg gagatgacgc
    16921 gtaaacgcac ccgcgaaagc attgagcacg tactgtgtaa cgaatgccca acctgccacg
    16981 gtcgcggaac ggtgaaaacc gtggaaacgg tatgctatga aatcatgcgc gagattgttc
    17041 gtgtccacca tgcttacgac tccgaccgtt tcctggtcta tgcttctccg gcagtagctg
    17101 aagccttgaa aggcgaagag tcacactcgc tggcggaagt ggaaattttc gttggtaaac
    17161 aggttaaagt acaaattgaa ccgctctata accaggagca gtttgacgtc gtaatgatgt
    17221 aaacagatgc tgggccgcca tccggcaaag ggtttttgag tcacatattt agcagacaag
    17281 gagtgacggg tgaggcgatt gccggggatt ttactgctta ctggagccgc gctcgttgtg
    17341 atcgctgccc tgctggttag cggcctgcgt attgctttac cgcatcttga cgcttggcgt
    17401 ccggaaatcc tcaacaaaat agaatccgcg actggcatgc cggttgaagc cagtcagctc
    17461 tcagccagct ggcagaattt tggcccgacg cttgaagcac acgacatccg tgcagaacta
    17521 aaagatggcg gcgaattttc ggttaaacgc gttactctgg cgctggatgt ctggcagagc
    17581 ctgttacata tgcgctggca gtttcgcgac ctcactttct ggcagctgcg ctttcgcacc
    17641 aacactccta tcaccagcgg tggtagcgat gacagtctgg aagccagtca catcagcgat
    17701 ctgtttcttc gtcaatttga ccatttcgat ctccgcgaca gtgaagtcag tttcctgacg
    17761 ccatccggtc agcgcgccga gctggcgatc ccacaactca cctggctgaa cgatccacgt
    17821 cgacaccgtg cggaaggcct ggtaagcctc tccagcctta ccggacagca cggcgtgatg
    17881 caggtgcgca tggatttgcg cgatgatgag gggttgttaa gcaatggtcg cgtctggctc
    17941 caggcggatg acatcgacct gaagccgtgg ctcggtaaat ggatgcagga caatattgcg
    18001 ctggaaacgg cacagttctc ccttgaaggc tggatgacga tcgataaagg cgatgtaacc
    18061 ggtggcgacg tctggctgaa acagggcggt gccagctggt tgggcgagaa ggaaacgcat
    18121 acgctgtcgg tggataatct gaccgcgcat attacgcgtg aaaatccggg ctggcagttc
    18181 tctattcccg atacacggat cacgatggac ggcaaaccct ggccgagcgg agcattgacg
    18241 ctggcctgga taccggaaca ggacgttggc ggcaaagaca ataaacgcag tgacgaactc
    18301 cggattcgcg ccagtaatct ggagctggca ggcctggagg gcatacgccc gctggccgcg
    18361 aaactttcac ctgcactggg tgatgtttgg cgctccacac aaccgagcgg caagattaac
    18421 acgctggcgc tggatatccc gcttcaggcg gcagacaaga cccgttttca ggcatcgtgg
    18481 agcgatctgg cctggaagca atggaaatta ttaccgggtg cggaacactt ctccgggacg
    18541 ctttccggca gcgttgaaaa tggtttgctt accgcgtcga tgaagcaggc aaagatgcct
    18601 tacgaaacgg tattccgtgc gccactggaa atcgccgacg gccaggcaac tataagctgg
    18661 ctgaacaatg acaaaggttt ccagctggat gggcgtaata ttgacgttaa agccaaagcc
    18721 gtccatgcgc gcggtggttt tcgttacctg caacctgcta acgatgaacc ctggctgggt
    18781 attctggctg gcatcagtac cgatgatggt tcacaagcct ggcgctattt cccggaaaac
    18841 ttgatgggta aagacctggt tgattactta agtggcgcga ttcagggcgg tgaagcggat
    18901 aacgcgacgc tggtttatgg tggcaatccg caactcttcc cctataaaca caacgaaggt
    18961 cagtttgaag tgctggtgcc gctgcgcaac gcgaagttcg ccttccagcc ggactggcct
    19021 gcattaacta accttgatat tgaactggac tttattaacg acggtttatg gatgaaaacc
    19081 gatggcgtta atctgggcgg cgtgcgcgcg agtaatctca ccgcagtgat ccctgactac
    19141 tcaaaagaaa aactgctgat tgacgctgac attaaaggtc cgggtaaagc cgttggccct
    19201 tactttgatg agacaccgct gaaagattct ctgggtgcga ccctgcaaga actccagctc
    19261 gacggcgatg tgaatgctcg cttacatctt gatatcccgc tgaacggcga gctggtaacc
    19321 gcgaaaggtg aagtgacgct gcgtaataac agtctgttta tcaaaccact cgacagcacc
    19381 ctgaaaaatt tgagcggtaa attcagcttt atcaatggcg atctgcaaag tgaaccactg
    19441 acagcaagct ggtttaatca gccgttgaac gtggattttt ccaccaaaga aggggcaaaa
    19501 gcctaccagg tagcggtaaa cctcaacggt aactggcaac cggcgaaaac cggcgttctg
    19561 cctgaagcgg tgaacgaagc attgagtggc agcgtggcgt gggatggtaa agtgggcatt
    19621 gatctgcctt atcatgctgg cgcgacctat aacgtagagc tgaacggcga tctgaagaat
    19681 gtgagcagtc acttaccttc accgttagcc aaacctgcgg gtgaaccact gccggtaaac
    19741 gttaaggttg atggcaatct caacagcttt gatttaaccg gacaggctgg tgcggataac
    19801 catttcaata gccgctggtt gctcggtcaa aagctcacgc tcgaccgtgc tatttgggcg
    19861 gcagacagta aaacgctccc gccgttgccg gaacaaagtg gtgttgaact caatatgccg
    19921 ccgatgaatg gtgccgagtg gctggccctg tttcagaaag gtgcggcgga gagtgtcggt
    19981 ggtgcagcga gtttcccaca acacataacg ttacgtacgc ctatgttgtc actgggaaat
    20041 cagcaatgga ataacctgag tattgtttcg caaccgacgg caaatggcac ccaggttgag
    20101 gcgcaagggc gtgaaatcaa cgccacgctg gcgatgcgta ataacgcgcc gtggctggcg
    20161 aatatcaaat atctttatta caacccgagc gtggcgaaaa ctcgtggtga ttcaacgccg
    20221 tcatcacctt tcccgacaac ggagcgcatt aacttccgtg gctggccgga cgcacaaata
    20281 cgatgcgcag agtgctggtt ctgggggcaa aaattcggtc gtattgacag tgatctcacc
    20341 atttctggcg atacattaac gctgaccaat ggactgattg ataccggttt ctcgcggctt
    20401 actgccgatg gtgaatgggt taataatccg gggaatgaac gtacctcgct gaaaggaaaa
    20461 ctgcgcgggc agaaaattga tgccgccgca gaattttttg gtgtcacgac gcccatacgg
    20521 cagtcgtcat ttaatgtgga ttacgattta cactggcgta aagcaccgtg gcagccagat
    20581 gaggcgacgt tgaatggcat cattcatact caactgggta aaggcgaaat taccgaaatc
    20641 aataccggac atgccgggca attgctgcgc ttattgagcg tagatgccct gatgcgtaag
    20701 ctgcgttttg atttcagaga cacttttggc gaagggttct attttgactc cattcgcagc
    20761 accgcgtgga ttaaagacgg cgttatgcac accgacgaca cgttggtgga tggcctggag
    20821 gcggatatcg ccatgaaagg gtcggtaaat ctggtacgtc gcgacctgaa tatggaagcg
    20881 gttgtcgcac cagagatttc tgcgacggtg ggcgtggctg cggcttttgc ggttaacccc
    20941 attgttggcg cggcagtgtt tgccgccagt aaagtgctgg ggccgctgtg gagcaaagtc
    21001 tccattttgc gctatcacat ttcgggtccg ctggacgatc cgcaaatcaa cgaagtgttg
    21061 cgccaaccgc gtaaagaaaa agcgcaatga tttgacgagg gcgcgtaatt gccccaatct
    21121 cataggataa tcgttgccaa aggccaacga gccagaacat aaccgtaggc tggatagggc
    21181 gttcacgccg catccggcag ccgtaaaaaa tcctctactg cagtaactaa cgagtagcaa
    21241 aaacgatgag tcttaacctg gtaagtgaac aattgctagc ggcgaacggc ctgaaacatc
    21301 aggacttgtt cgcgatcctc ggtcaactgg ccgaacgtcg ccttgattat ggcgatctct
    21361 attttcagtc gagctatcac gaatcctggg ttttagaaga ccgcattatt aaagatggtt
    21421 cttacaacat cgatcagggc gttggtgtgc gtgcaatcag cggtgaaaaa accggatttg
    21481 cttacgctga ccaaatcagc ctgctggcgc tggaacagag tgcgcaagcg gcgcgcacca
    21541 tcgtccgtga tagcggtgat ggcaaagtac agacgctggg cgcggtagag catagcccgt
    21601 tgtatacctc ggtagatccg ctgcaaagca tgagccgtga agagaagctg gatatcctac
    21661 gtcgcgtcga taaggttgcc cgcgaagcgg acaagcgcgt acaggaagtg actgccagcc
    21721 tcagtggtgt ctatgaatta attctggttg cggccaccga cggcacgcta gcggcggatg
    21781 tccgtccgct ggtgcgtctt tccgtgagcg ttctggtcga agaagatggc aaacgcgaac
    21841 gcggtgccag tggcggcggc ggtcgttttg gttatgagtt cttccttgcc gatcttgacg
    21901 gcgaagtccg tgcggatgca tgggcaaaag aagcagtgcg tatggcgctg gtcaatcttt
    21961 ctgccgttgc tgcaccagcg ggcaccatgc cggtagtact tggcgcaggt tggccgggcg
    22021 tgctgttgca tgaagcggta ggtcacggtc tggaaggcga cttcaaccgc cgtggcactt
    22081 cagtatttag tggacaggtc ggggagctgg tggcttcaga actgtgtacc gtggttgatg
    22141 acggtacgat ggtcgatcgc cgtggttcgg tggcgattga tgacgaaggt acgccaggcc
    22201 agtacaacgt gctgattgag aacggcattc tgaaaggcta catgcaggat aaactcaacg
    22261 cgcgtttgat ggggatgacg ccgactggca acggtcgccg tgaatcctac gcccatctgc
    22321 ccatgccgcg tatgaccaac acctatatgc tgccgggtaa atcgaccccg caagaaatta
    22381 ttgaatccgt tgagtacggt atctatgcac cgaactttgg tggcggtcag gtggatatca
    22441 cctccggcaa attcgttttc tccacttcag aagcatatct gattgaaaac ggtaaagtaa
    22501 cgaagccggt gaaaggcgca acgttgattg gttccggtat cgaaaccatg cagcagattt
    22561 cgatggttgg caacgacctg aaactggata acggcgtggg tgtctgcggt aaagaagggc
    22621 aaagtttgcc ggttggcgtg ggccagccaa cgctgaaagt tgataacctg actgttggcg
    22681 gtactgcgta ataatttcag tattttcaga aggataatta atctttctac gtgcacgaac
    22741 ggtcccctcg ctcctctggg gttagggtga ggggaacccg ttggcacagg tttgtacaac
    22801 gtaacagtac aatatgaatt acttctcttt cccgcgcccg tgcatctcct gaaacaattt
    22861 accgacctca acaaaataat ccgtcagcga gttgatcacg acctgtacct tcagcggcag
    22921 cttatctttt tcggtatata acgcataaac cgggcgtgga tctgactgat aacgcggcag
    22981 caggatctcc agctccccac gattgatctc gttgattacc cacatcagcg gcacgtaggc
    23041 gatcccggca cccgccgtta gccagcgcac cagcgtcatc ggatcattag tcacaaatct
    23101 cccttgcggg atcaggcgag tcgagatccc ttccggtgca atcagttcaa attcattgtc
    23161 gggccgcacg ctgtattcaa gccatgaatg gctacttaaa tcggcgggtt tttccggtat
    23221 gccgtattgt gtgagatagc ttttcgcggc gcacaccacc attggcatcg cgccaagacg
    23281 gcgggaaaac aggctggaat cctgcaacgc gccgacgcgg atcaccacat ccagaccgtc
    23341 ggcaatcagg tcgggggcag gaattccggt aaccagattg acactcaaac ctgggtattc
    23401 tttcaacatt tttgctgtca gcccggcaag aacattttgt gccatagttg aagaacagcc
    23461 aatgcgcagc gtcccgatgg gggtgttatt gaaggcatac agttgctcat gaacatcctg
    23521 cacttcatga agcatccgac ggcagccctg gtagtaaatt ctaccggctt cggtcaggcc
    23581 aatgctgcgt gtactacggt ttaacagctt tacctgcaac tcatcttcca gttttgacac
    23641 cgtctgactg atggacgaaa cgctcatctg tagctgtctg gcggcggcgg taaaagagcc
    23701 aaattcaact actttggcaa acaccgacat gcgttttagt cgttccatta ttcactctga
    23761 cttaaaagtg atttagatca cataatatag ataacagcat aacagttacg ctaatatatt
    23821 aaatatcaat ctacagcaat gttgctctcg cccggctttc catgcccttc tctgcggcga
    23881 cagatgctga aaataataac gcctgctctc tctatacaac caaggtcaac atgagtctgt
    23941 ttcccgttat cgtggtgttt gggctgtcct tcccaccgat attttttgaa ttgcttttat
    24001 cactggcgat tttctggctg gtgcgccggg tacttgtgcc aacaggtatc tacgactttg
    24061 tctggcatcc ggcgttgttc aacaccgcgc tctattgctg cttgttttat ttgatatcgc
    24121 gactgttcgt ttgaggttga agtgaaaaca ctaataagaa aattctcccg tacggccatc
    24181 acggtcgtat tagtcattct ggccttcatc gcaattttta atgcctgggt ctattacacc
    24241 gaatccccgt ggacgcgtga cgcgcgcttt agcgctgacg tcgttgcgat cgcgccggac
    24301 gtttctggac tcattaccca ggtgaatgtt catgataacc agctggtgaa aaaaggacag
    24361 gtactgttca ccatcgacca gccgcgctat caaaaggcgc ttgaggaagc gcaagccgat
    24421 gttgcttatt atcaggtatt ggcacaggag aaacgccagg aggccggacg tcgtaaccgt
    24481 ctcggtgtgc aggcgatgtc tcgcgaagag atcgaccagg ccaacaacgt actacaaacg
    24541 gttctgcatc agttagcgaa agcgcaagcg acccgcgatc tggcaaaact ggatcttgaa
    24601 cgcacggtga tccgcgcgcc agcggatggc tgggtgacca acctcaacgt ctataccggt
    24661 gagtttatta ctcgtggatc aacggcggtt gcgctggtga aacagaactc tttctatgta
    24721 ctggcctata tggaagaaac taagctggaa ggggtgcgcc ctgggtatcg tgcagagatc
    24781 acgccgcttg gcagtaacaa agtgctgaaa gggactgttg atagtgttgc cgctggggtc
    24841 accaacgcca gcagcacgcg tgacgacaaa gggatggcga ccatcgactc taaccttgaa
    24901 tgggtgcgtt tggcgcaacg tgttccggtt cgtattcgtc tcgacaacca gcaagagaac
    24961 atctggcctg cgggcaccac tgctacagtg gtggtcactg gcaaacaaga tcgcgacgaa
    25021 agccaggatt cgttcttccg taaaatggcc catcgcctgc gtgagtttgg ttaatcacga
    25081 tgggtatttt ctccattgct aaccaacata ttcgctttgc ggtaaaactg gcgaccgcca
    25141 ttgtactggc gctgtttgtt ggctttcact tccagctgga aacgccacgc tgggcggtac
    25201 tgacagcggc gattgttgcc gccggtccgg cctttgctgc gggaggtgaa ccgtattctg
    25261 gcgctattcg ctatcgtggc tttttgcgca tcatcggcac atttattggc tgtattgccg
    25321 gactggtgat catcattgcg atgatccgcg caccattatt gatgattctg gtgtgctgta
    25381 tctgggccgg tttttgtacc tggatatcct cgctggtacg aatagaaaac tcgtatgcgt
    25441 gggggctggc cggttatacc gcgctgatca ttgtgatcac cattcagccg gaaccattgc
    25501 ttacgccgca gtttgccgtc gaacgttgta gcgagatcgt tatcggtatt gtgtgtgcga
    25561 ttatggcgga tttgctcttt tctccgcgat cgatcaaaca agaagtggat cgagagctgg
    25621 aaagtttgct ggtcgcgcaa tatcaattaa tgcaactctg tatcaagcat ggcgatggtg
    25681 aagttgtcga taaagcctgg ggcgacctgg tgcgacgcac cacggcgcta caaggcatgc
    25741 gcagcaacct gaatatggaa tcttcccgct gggcgcgggc caatcgacgt ttaaaagcga
    25801 tcaatacgct atcgctgacg ctgattaccc aatcctgcga aacttatctt attcagaata
    25861 cgcgcccgga attgatcact gatactttcc gcgaattttt tgacacgccg gtagaaaccg
    25921 cgcaggacgt ccacaagcag ctcaaacgcc tgcgaagagt tatcgcctgg accggggaac
    25981 gggaaacgcc tgtcaccatt tatagctggg tcgcggcggc aacgcgttat cagcttctca
    26041 agcgcggcgt tatcagtaac acaaaaatca acgccaccga agaagagatc ctgcaaggcg
    26101 aaccggaagt caaagtagag tcagccgaac gtcatcatgc aatggttaac ttctggcgaa
    26161 ccacactttc ctgcattctg ggaacgcttt tctggctgtg gacgggctgg acttccggca
    26221 gtggtgcaat ggtgatgatt gcggtagtga cgtcactggc aatgcgtttg ccgaatccac
    26281 gcatggtggc gatcgacttt atctacggga cgctggccgc gctgccgtta gggctgctct
    26341 actttttggt gattatccct aatacccaac agagcatgtt gctgctgtgt attagcctgg
    26401 cagtgctggg attcttcctc ggaatagaag tacagaaacg gcgactgggc tcgatggggg
    26461 cgctggccag caccataaat attatcgtgc tggataaccc gatgactttc catttcagtc
    26521 agtttctcga cagcgcatta gggcaaatcg tcggctgtgt gctcgcgttc accgttattt
    26581 tgctggtgcg ggataaatcg cgcgacagga ctggacgtgt actgcttaat cagtttgttt
    26641 ctgccgctgt ttccgcgatg actaccaatg tggcacgtcg taaagagaac cacctcccgg
    26701 cactttatca gcagctgttt ttgctgatga ataagttccc aggggatttg ccgaaatttc
    26761 gcctggcgct gacgatgatt atcgcgcacc agcgcctgcg tgatgcgccg atcccggtta
    26821 acgaggattt atcggcgttt caccgacaaa tgcgccgcac agcagaccat gtgatatctg
    26881 cccgtagcga tgataaacgt cgtcggtact ttggccagtt gctggaagaa cttgaaatct
    26941 accaggaaaa gttacgcatc tggcaagcgc caccgcaggt gacggaaccg gttcatcggc
    27001 tggcggggat gctccataag tatcaacatg cgttgaccga tagttaagtc aaaaccgacg
    27061 ccaaaagcgt cggttttttc atggctatac ttagcgatga acggcagaac tcgccgcgaa
    27121 acgtgacggt ggcaacagat gaatatttat acctttgatt ttgatgagat tgagagtcag
    27181 gaggattttt atcgcgactt cagccaagcc tttggtctgg cgaaagataa ggtacgcgat
    27241 ctcgactcac tatgggatgt gttaatgaac gatgtcctgc cgctaccact tgagattgaa
    27301 tttgttcatc tgggagagaa aacgcgtcgc cgttttggcg cgttaatatt gctgtttgat
    27361 gaggcagagg aagagctgga agggcatttg cgttttaatg ttcgtcatta gcgcaaaaaa
    27421 aagcccccga accgggggca atatcgtcgg acaagacgat gagggtttat ttgtacagtt
    27481 cagccgtagc gtgccaggtg tcaccgctac gagcttccgt aatctggtag gccgttgcgc
    27541 ctttctcttc cgcttttttg ttcagcattt cacgcatatc cattggcgaa gacgccacac
    27601 cacttacgga tacggtcccg attgcttcac gattttgtgc ttgtgcagca tcaatggagt
    27661 cggcagcgaa tgcaccgaaa gagagaacag aaagtacgct taatgcagca acagtggttt
    27721 tgattttcat gatttttacc tcgacataat ctttttgctg ggtctttgtt tcgtgaccct
    27781 tatcacaaaa tcaagtatac actaattatt ggactaatta at
//
LOCUS       DAPGWD010000050        27404 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPGWD010000050 DAPGWD010000000
VERSION     DAPGWD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.050"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2120
                     /locus_tag="RHX88_002068"
     CDS             <1..2120
                     /locus_tag="RHX88_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HDV2230757.1"
                     /translation="TEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVY
                     HQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTL
                     RINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLI
                     IYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGSLLNASDKLFYLPFAFII
                     IVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSI
                     AEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVF
                     GVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSS
                     FGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEEN
                     IWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAE
                     DIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMK
                     ETGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEIN
                     PDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQA
                     QGYLYQKPMPFSAWDKSGKLVKE"
     gene            2128..3456
                     /locus_tag="RHX88_002069"
     CDS             2128..3456
                     /locus_tag="RHX88_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV2230758.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIVEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(3503..4828)
                     /gene="rimO"
                     /locus_tag="RHX88_002070"
     CDS             complement(3503..4828)
                     /gene="rimO"
                     /locus_tag="RHX88_002070"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDV2230759.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            5041..5424
                     /gene="bssR"
                     /locus_tag="RHX88_002071"
     CDS             5041..5424
                     /gene="bssR"
                     /locus_tag="RHX88_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDV2230760.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            5535..6650
                     /gene="yliI"
                     /locus_tag="RHX88_002072"
     CDS             5535..6650
                     /gene="yliI"
                     /locus_tag="RHX88_002072"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDV2230761.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(6647..7273)
                     /gene="gstB"
                     /locus_tag="RHX88_002073"
     CDS             complement(6647..7273)
                     /gene="gstB"
                     /locus_tag="RHX88_002073"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDV2230762.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            7519..8721
                     /gene="dacC"
                     /locus_tag="RHX88_002074"
     CDS             7519..8721
                     /gene="dacC"
                     /locus_tag="RHX88_002074"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV2230763.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(8768..9526)
                     /gene="deoR"
                     /locus_tag="RHX88_002075"
     CDS             complement(8768..9526)
                     /gene="deoR"
                     /locus_tag="RHX88_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDV2230764.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(9584..10180)
                     /gene="ybjG"
                     /locus_tag="RHX88_002076"
     CDS             complement(9584..10180)
                     /gene="ybjG"
                     /locus_tag="RHX88_002076"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV2230765.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            10465..11697
                     /gene="mdfA"
                     /locus_tag="RHX88_002077"
     CDS             10465..11697
                     /gene="mdfA"
                     /locus_tag="RHX88_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDV2230766.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(11738..12022)
                     /locus_tag="RHX88_002078"
     CDS             complement(11738..12022)
                     /locus_tag="RHX88_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230767.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(12108..12923)
                     /gene="ybjI"
                     /locus_tag="RHX88_002079"
     CDS             complement(12108..12923)
                     /gene="ybjI"
                     /locus_tag="RHX88_002079"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDV2230768.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(12923..14131)
                     /locus_tag="RHX88_002080"
     CDS             complement(12923..14131)
                     /locus_tag="RHX88_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2230769.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14215..14751
                     /gene="rcdA"
                     /locus_tag="RHX88_002081"
     CDS             14215..14751
                     /gene="rcdA"
                     /locus_tag="RHX88_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDV2230770.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(14926..16611)
                     /locus_tag="RHX88_002082"
     CDS             complement(14926..16611)
                     /locus_tag="RHX88_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDV2230771.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            16881..17258
                     /locus_tag="RHX88_002083"
     CDS             16881..17258
                     /locus_tag="RHX88_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDV2230772.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(17288..17545)
                     /gene="grxA"
                     /locus_tag="RHX88_002084"
     CDS             complement(17288..17545)
                     /gene="grxA"
                     /locus_tag="RHX88_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDV2230773.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            17705..17992
                     /locus_tag="RHX88_002085"
     CDS             17705..17992
                     /locus_tag="RHX88_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDV2230774.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            17976..18698
                     /gene="nfsA"
                     /locus_tag="RHX88_002086"
     CDS             17976..18698
                     /gene="nfsA"
                     /locus_tag="RHX88_002086"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDV2230775.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            18759..19661
                     /gene="rimK"
                     /locus_tag="RHX88_002087"
     CDS             18759..19661
                     /gene="rimK"
                     /locus_tag="RHX88_002087"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDV2230776.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            19749..20225
                     /locus_tag="RHX88_002088"
     CDS             19749..20225
                     /locus_tag="RHX88_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDV2230777.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            20576..21688
                     /gene="potF"
                     /locus_tag="RHX88_002089"
     CDS             20576..21688
                     /gene="potF"
                     /locus_tag="RHX88_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDV2230778.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     NLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            21783..22916
                     /gene="potG"
                     /locus_tag="RHX88_002090"
     CDS             21783..22916
                     /gene="potG"
                     /locus_tag="RHX88_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDV2230779.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            22926..23879
                     /gene="potH"
                     /locus_tag="RHX88_002091"
     CDS             22926..23879
                     /gene="potH"
                     /locus_tag="RHX88_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDV2230780.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            23876..24721
                     /gene="potI"
                     /locus_tag="RHX88_002092"
     CDS             23876..24721
                     /gene="potI"
                     /locus_tag="RHX88_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDV2230781.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            24781..25269
                     /locus_tag="RHX88_002093"
     CDS             24781..25269
                     /locus_tag="RHX88_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDV2230782.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            25310..26437
                     /gene="rlmC"
                     /locus_tag="RHX88_002094"
     CDS             25310..26437
                     /gene="rlmC"
                     /locus_tag="RHX88_002094"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDV2230783.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(26612..27343)
                     /gene="artJ"
                     /locus_tag="RHX88_002095"
     CDS             complement(26612..27343)
                     /gene="artJ"
                     /locus_tag="RHX88_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDV2230784.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT         6775 a         6566 c         7079 g         6984 t
ORIGIN      
        1 atacagagga gattcaacgc gccgtgattc tgaaaatgga aatgatgcca tttttagact
       61 cggtgggact ggtacttgat gataataaat attatctctt ttcgcggagg gcgaatgata
      121 aaatcgttgt ttatcatcag gaacaagtaa atggaccgct tgtcgacgag tcagggcggg
      181 ttatttttgc cgattttaac ccatcgaaac gaccgtggtc ggtggcttca gatgactcta
      241 acaacagctg gaatccggca tacaattgct ttgatcgtcc gggtaaaaaa tgtatctctt
      301 ttacgctacg tatcaacggc aaagatcacg atttgttagc ggtagataaa attcatgtcg
      361 atttaaactg gcggtatctg aacgagtatc ttgatcaaat cagcgctaat gatgaagttc
      421 tatttttgaa acaaggtcat gagatcattg ccaagaatca actcgctcgt gaaaaactga
      481 ttatttataa tagcgaaggt aattataata ttattgattc tgtcgatacg gagtatattg
      541 caaagacatc agcagtgcca aataatgcat tgttcgaaat ctatttttat tatcctggcg
      601 gtagtttatt gaacgcatca gataaacttt tttatctacc gtttgcgttc attattatcg
      661 tgttgctggt ggtatatttc atgaccactc gtgtgttccg tcggcaattt tctgaaatga
      721 ccgagctggt taatacgctg gcgtttttgc ccgactcaac ggatcagatc gaggctctga
      781 aaattcgcga aggcgatgcg aaagagatta tcagcatcaa aaattcgatc gcggaaatga
      841 aagatgccga aattgaacgg tcaaataaat tgctctcact gatctcttac gatcaggaaa
      901 gcggttttat taaaaatatg gcgattattg agtccaacaa taatcagtat ctggctgtgg
      961 ggatcatcaa actgtgtggt ctggaagccg tggaagcggt gtttggtgtt gatgaacgca
     1021 ataaaatcgt caggaaattg tgtcagcgaa ttgccgagaa atatgcgcaa tgctgcgata
     1081 tcgtgacatt caatgccgat ctctatttac ttctgtgtcg ggaaaatgta cagacattta
     1141 cccgtaaaat agcgatggta aacgattttg acagcagctt tggctaccgc aatctgcgca
     1201 tccataagtc tgccatttgt gaacctttgc agggggaaaa cgcctggagt tacgcagaaa
     1261 aactgaaact ggcgatttcc agtatccgtg accatatgtt ctccgagttt attttctgtg
     1321 atgacgcgaa actcaacgaa atagaagaga atatctggat tgcgcgtaat attcgccatg
     1381 caatggaaat tggcgaacta ttcctcgtct atcaaccgat cgttgatatt aacacccgcg
     1441 ccattctggg cgcggaggcg ttgtgccgtt gggtttctgc ggagcggggg atcatttcac
     1501 cgctgaagtt cattaccatt gctgaagata tcgggtttat caatgagctg ggttatcaga
     1561 ttattaaaac ggcaatgggt gaattcagac attttagtca gcgtgcgtcg ctgaaggatg
     1621 atttcttact gcatattaat gtttcgccct ggcagttaaa cgaaccgcac tttcatgagc
     1681 gttttaccac catcatgaaa gaaactggcc tgaaggcgaa cagcctctgt gttgagatca
     1741 ctgaaaccgt gatcgagcga attaatgaac atttttatct caatattgaa caactgcgta
     1801 aacaaggggt acggatatcg attgatgact ttggcaccgg tttgtcaaac ctgaaacgtt
     1861 tttatgaaat taatccagac agcataaagg tggactcgca atttaccggc gatattttcg
     1921 gtactgcggg aaaaattgtg cgcattattt tcgatctggc acgctataac cggatcccgg
     1981 tgattgcgga aggcgtagag agcgaagacg ttgcgcgcga attaattaaa ttaggatgtg
     2041 ttcaggctca ggggtatctg taccagaaac ccatgccatt ctccgcctgg gataaaagtg
     2101 gaaaattagt aaaagagtag tttacgtatg tccagaatca ataagttcgt acttacagtc
     2161 agtctgctga tttttatcat gatttcagca gttgcctgcg ggatctacac tcaaatggta
     2221 aaggaacggg tgtatagcct gaaacagtcc gttattgata ctgcttttgc ggtggcaaat
     2281 attgttgaat atcggcgtag cgtggcaatt gatcttatca acacgctaaa tcccacggag
     2341 gaacagctgt tggttggttt gcgcacagct tacgccgact cggtttcccc ctcttatttg
     2401 tacgatgtcg gtccttatct gatttccagt gacgaatgta ttcaggtaaa ggagttcgag
     2461 aaaaattatt gtgcagatat tatgcaggtt gtgaagtatc gacatgtcaa aaatacaggg
     2521 tttatctctt ttgacggtaa aaccttcgtc tattacctct atccggtaac tcacaatcgt
     2581 agtctgatat ttttgcttgg tctggagcgt ttttctttac tgtcaaaatc gctggcgatg
     2641 gacagcgaga acctgatgtt ctctctattt aagaacggta aaccggtgac cggtgatgaa
     2701 tataatgcta aaaacgccat cttcaccgtt tcggaagcga tggagcactt cgcctatttg
     2761 ccgaccggat tgtacgtatt tgcgtataaa aaagatgttt atttgcgggt ctgtacattg
     2821 attattttct ttgccgcatt ggtggcagtg atatcgggta ccagttgcct ctatctggta
     2881 cgcagagtga ttaatcgtgg tattgtggag aaagaagcca tcattaataa ccattttgaa
     2941 cgcgtactgg atggcgggct tttcttttcg gctgccgatg tcaaaaaact ctacagtatg
     3001 tataactcgg cgttcctgga cgacctgacc aaagcaatgg gcagaaaatc ctttgacgaa
     3061 gatttaaaag cgctgccgga aaaaggcggt tatttgtgcc tgtttgacgt cgataaattc
     3121 aaaaacatta acgacacctt cggtcatttg ctgggcgatg aagtgttgat gaaagtggtg
     3181 aaaatcctta aatcacagat cccggtagat aaaggtaaaa tctaccgctt cggcggtgac
     3241 gaatttgcgg tgatttatac gggtggaacg ctggaagagt tgctatcgat tctaaaagaa
     3301 atcgttcatt tccaggtggg aagcattaat ttaagtacca gtatcggtgt agcgcattca
     3361 aatgaatgta ccaccgtcga acgcttgaaa atgctggcgg atgagcggct gtataagagt
     3421 aagaaaaacg gcagggcaca gattagctgg cagtaatcat tattcgcagg ccggacaaat
     3481 gattttgccc ggcctgaatt aattaaaccc ggctacccca caaatcgtac tcatcggcgt
     3541 gctcgacttt cacacgcagg atatcacccg gcttaacgtt ggtttcacca ttgagataaa
     3601 ccgcgccgtc gatttccggt gcatctgcca tgctgcgacc aatcgcgcct tcttcgtcca
     3661 cttcgtcgat aatcaccaga atttcacggc ccactttctc ttgcaggcgc tcggcggaaa
     3721 tctgctgctg cagctgcatg aaacggttcc agcgttcttc tttaacttct tccggaacct
     3781 ggtcaggcag ggcattggcg tctgcacctt caaccgggct gtatttaaag cagccaacgc
     3841 gatccagacg cgcttctttc aggaagtcga gtagcatctg gaaatcttct tctgtctcgc
     3901 cagggaagcc gacaataaag gttgagcgta gggtcagttc cgggcagatt tcgcgccact
     3961 gtttgatgcg cgccagttgg cgatctacag aacccggacg cttcatcagt ttgagaatgc
     4021 gcgggctggc gtgctgcaac ggaatgtcca gatacggcag gattttgcct tctgccatca
     4081 gtgggatgac gtcgtccaca tgcggataag ggtaaacgta gtgcagacgt gtccagatcc
     4141 ccagtttcga taactgttcg cacaggctga ccatgctggt ttttaccggc tcgccgttgt
     4201 ggaagccagt acgatgttta acatcaacgc cataggcgga agtatcctgc gagatcacca
     4261 gaatctcttt aacgcccgca tctaccagac gtttcgcttc acttaacact tcgccaatcg
     4321 gacggctcac caggtcgccg cgcatagacg gaataatgca gaaggtgcag cggtgattac
     4381 agccttccga aattttcaga taggcataat gacgcggcgt cagtttcaca ccttgttctg
     4441 gcaccaggct caggaatggg ttgtgtttcg gttttggcac gtagtgatga acgtgctcca
     4501 gaacctgctc atagctatga ggcccggtga tttccagcac tttcgggtgg acttcgcgga
     4561 tctgatcttc ttttgccccc agacaaccgg tcacaattac cttgccgttt tcattcaacg
     4621 cttcaccaat ggcttccagt gattcttgta ccgcgctgtc aataaagccg caggtgttga
     4681 cgatcaccat gtccgcatcg tcatagctcg gtaccacgtc ataaccttca gtgcggagtt
     4741 cggtgagaat acgctctgaa tcaacaaggt tttttggaca gccaagggaa acaaagccga
     4801 ttttcggctg gggagttact ttgctcatag cttaaaaaat attcagttac aggaaaggtc
     4861 agggcaggga ttctacagag ttctgcataa aatttgtatc gcaatctcat tcgctggcgg
     4921 aggcgaagga aatgtaaatt ttgttaattc ggcgtgaaga attgatcctg gacagcattt
     4981 tgctcaaaaa atagccatac tatttaattg caacaaggct ggaaagagga ggatcgaagt
     5041 atgttcgttg acagacagcg aatcgatctg ctgaaccggt tgatcgacgc acgcgttgac
     5101 ctcgccgcat acgtgcaact gaggaaggca aaaggataca tgtccgtcag cgaaagcaat
     5161 catctacgag ataacttttt taaactgaat cgcgaactgc acgataaatc gctgcggttg
     5221 aatcttcatc tggatcagga agagtggagt gctcttcatc atgctgaaga agcattagcg
     5281 acagccgcag tatgtttgat gagtgggcac catgattgcc cgactgttat taccgtcaac
     5341 gccgataagc ttgaaaattg tctgatgagc ttaacgctga gtatccagag cctgcagaag
     5401 cacgccatgc ttgagaaggc ctgaaaacta agggggagaa agcgtctccc ccttcatgtt
     5461 taagtttttg taaaaatgaa tttgttatct cctccactga ctacgcttta agccagagtc
     5521 aatccggagg cgttatgcat cgacaatcct ttttccttgt gccccttatt tgtctttctt
     5581 ccgctctctg ggcggctcct gcaacggtaa atgtcgaagt actgcaagac aaactcgacc
     5641 atccctgggc actggccttt ttacccgata atcacggtat gttaatcact ctgcgcggcg
     5701 gcgagttgcg tcactggcaa gcaggaaaag gattatctgc gccgctttcc ggagttccgg
     5761 acgtttgggc gcacgggcag ggcggcctgc tggacgtggt tttagcgcct gattttgctc
     5821 agtctcgccg catctggtta agttattccg aagttggcga tgatggcaaa gccggaactg
     5881 ctgtgggtta tggccgctta agtgatgatc tctcaaaagt gaccgacttc cgcaccgtct
     5941 tccgccagat gccaaaactg tctaccggca accattttgg cgggcggctg gtattcgacg
     6001 gtaaaggtta tctttttatt gctctgggcg aaaacaatca gcgcccgacg gcgcaggatc
     6061 tggataaatt acagggcaaa ctggtgcgtc tgaccgacca gggcgaaatc ccggatgata
     6121 atccttttat aaaggaatcc ggtgtgcgcg ccgagatctg gtcttatggc attcgtaatc
     6181 cgcaaggaat ggcgatgaat ccgtggagta atgcactgtg gctgaatgaa catggcccgc
     6241 gcggtggtga tgaaattaat atcccgcaaa aaggcaaaaa ctacggctgg ccgctggcaa
     6301 cctggggaat caactattca ggctttaaga taccggaagc gaaaggggag atcgtcgccg
     6361 ggaccgagca acctgttttt tactggaaag attcgcccgc tgtgagcggc atggccttct
     6421 ataacagcga taaattcccc cagtggcagc aaaaattatt tattggcgcg ctgaaagata
     6481 aagatgtcat tgtgatgagc gtcaacggcg acaaagtgac agaagatggc cgtattttaa
     6541 cggacagagg gcagcgaatt cgtgatgttc gcactggacc cgacggttat ttatacgttc
     6601 tcaccgacga gtccagtggg gaattactta aagttagccc acgcaattag ctaacgggaa
     6661 tcatcaccac tttgcgcact gcggggcgtt cagtgagttg ctgataccag cgttgcagat
     6721 ttggacgcgg tgtccaggtc aggccaacgt taaacaaatt gtagatgaac ggtgcgatag
     6781 cgatatcgcc cacaccaaac tcgtcgccag agaaccattt tacttttgcc agttccgcat
     6841 cgagcagggc aaacagggcg tcgcactctt tgcaactggc atcaatggcg gcctgatcgc
     6901 gctcttccgg tggtgttctg actaatccca tcaggatccc gcgatgagca ttactgagcg
     6961 tctggtttgc ccagtccatc catttttccg cttccgcacg acgtgccggt gagtcgatcc
     7021 acagacgttt ttgcccgtac tgtgccgcca gatagcggac aatggcgttt gattcccaaa
     7081 gaatgagatc actttcgtcg tcacgcaaca acggcaccag cccgttaggg ttcatcgcca
     7141 gaaaatcagc atcgtgattt atcccaaact cacggcccgc gagaatttgc tcataaggta
     7201 gttccagttc ttcgagcgtc agcaatactt tttttacgtt agttgaatta ttccgacccc
     7261 acagcgtaat catactcacc ccttttccga caggcagcca caggctgaac aggactccga
     7321 tggtgaagta aagttaactt ttaagcaaca gctggcaaaa aaatgacacc agaagcagcg
     7381 aagcggcagg atattgcata aactttaaaa actttaccaa cttacggttt ctttaagttt
     7441 gtgtgtgcgt tattaatcac cgaacttatc atacggcgat ataacgtatt tttttgaatg
     7501 gatactcggg tggcatttat gacgcaatac tcctctctcc ttcgtggtct tgcagcgggt
     7561 tctgcatttt tattcctttt tgccccaacg gcattcgcgg cggaacaaac cgttgaagcg
     7621 ccgagcgtgg atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca
     7681 gaaggcaacg cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat
     7741 gtggttgggc aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt
     7801 aaagatgcct gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa
     7861 ccgggcgatc aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat
     7921 gacgcctgta ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg
     7981 atgaatggtt atgccaaaaa actgggtctg accaacacta ccttccagac ggtgcacggt
     8041 ctggatgcgc cggggcagtt cagtaccgcg cgcgatatgg cattgctggg taaagcattg
     8101 atccacgatg tgccggaaga gtacgccatt cataaagaga aagagttcac cttcaacaaa
     8161 attcgtcagc ctaaccgtaa ccgtctgctg tggagcagca atctgaatgt tgatggcatg
     8221 aagacaggaa ccacggcagg cgcgggatat aatctggttg cttcggctac ccagggcgat
     8281 atgcgtttaa tctccgtagt gctgggggcg aaaactgacc gtatccgttt taatgagtct
     8341 gagaaattat tgacctgggg tttccgcttc tttgaaaccg tgacgccaat taaacctgat
     8401 gccacctttg tgactcagcg cgtctggttt ggtgataaga gcgaagtgaa tcttggggca
     8461 ggtgaagcgg gctcagtgac cataccgcgt gggcagctga aaaacctgaa ggcgagctac
     8521 acgttaacgg aaccgcagct taccgcaccg ctgaaaaaag gtcaggttgt cgggaccatt
     8581 gatttccagc ttaacggtaa atccattgag cagcgtccgc tgatcgtgat ggaaaatgtg
     8641 gaagagggcg gattctttgg tcggatgtgg gatttcgtga tgatgaaatt ccatcagtgg
     8701 ttcggcagct ggttctctta atcttctgat aaccggatgg cgcgaaacgt catccggtta
     8761 tacgtcatta atacatcaac ttaatacggt gcgtctgcgc gtacttcaca tactcatctt
     8821 ccgggcaaca atcactcacc acaatatcaa agcgtttcag gtcacccatg cgcgccggac
     8881 gcaccttgcc aaatttactg tggtcgacaa ccagcacatg cttttgcgcc atcgacatgg
     8941 cccagtgttt taccggcaac tcttcaagat taaaacaggt agcgccttta ctgacatgca
     9001 cgcccgccgc ggaataaaaa gcgatatccg ggcaaaaatt attcaacgtt tgctgaaaat
     9061 cgatgggttt gaaaatggcg ttgctggcgt gaaattcacc accgcacaga aatgcgcggc
     9121 aatggggttt ctctttcagc gccagaaagg tatttagcga ataacaaacg gcggtaaaag
     9181 ggatttcatt atcaatcgct tcaataatcc acggcgtggt ggtgccacag tcaaaaaaga
     9241 gagtctgatc gggttctacc agtgtcgcag ccatttttgc cgcccggcgt ttttcttcca
     9301 ccaggcggga tttttgatcg cttaacaggt aatggctggc actgcgcggt tccagaacaa
     9361 tatagccgcc gagcaaaacg acgggcgcac tgtggttgtt cagatcgcga cgaatcgtca
     9421 tctccgaaac cccaagcagg gcggcggcgt ctttaagatg taacttatcg ctgcgtttta
     9481 attcttgcag cagctgcccg atacgctctt cgcgacgtgt ttccataatc cctctgaata
     9541 gttattgaag cgagccgctc aatactacac tttttagcag agatcagtca cgcacccagc
     9601 ctttgcggat cggtaatgca aaacagaagc gataccacga ttgcagacgt tgatagagtt
     9661 tatgccccat cgcttgccag ataatctgag cgctaaggca gccaatcata cctgccagca
     9721 atccaccgag catatccagc ggccagtgca cgccaagata aacgcgcgac caggcaatga
     9781 cgacggccag caccattaaa agtgagccgg accacaggcg atgccagcat aaaaatgcca
     9841 gtgcaaaggt gaaaatcacc gtaccgtggt cgcttgggaa tgagtcatcc gccgcatgat
     9901 gcaggaagtt atagccgata ttttcgacaa agggtcggtc gtgcggaaaa agatgtccca
     9961 tcgtccagga cacaaacagg ctgaccgcca gcgcgatagc gatttttatc accagttgcc
    10021 gttgtgctgt aagcccccac aaccaaagca ccgcggccag cagcggtacc acggtaatca
    10081 aatctttagc aataaaaatc gccaacgaga tcatccacgg agccgagtct ggcgtcgcgt
    10141 taataagaga gaatagagag agattcaaat tttccagcat aacttcccga cgcaaagtga
    10201 ttaaaaggga gccaatacag gcaagtcgtt gagaataaag tgcaggttaa actgggtaaa
    10261 gcggcatcgt cttatttccc tcaagcggcc tgtttacggt gggtaattgt aacgggcata
    10321 ggttaaataa aacttaaaga aagcgtagct atactcgcaa taatgtaaga atgtgcttaa
    10381 ccgtggtttc agctacaaaa ttcgctttct cgttagctgt gcttttatta aactctgcgc
    10441 gattattatt ggcgaagaaa ttgcatgcaa aataaattag cttccggtgc caggcttgga
    10501 cgtcaggcgt tacttttccc tctctgtctg gtgctttacg aattttcaac ctatatcggc
    10561 aacgatatga ttcaacccgg tatgttggcc gtggtggaac aatatcaggc gggcattgat
    10621 tgggttccta cttcaatgac cgcatatctg gcgggcggga tgtttttaca atggctcctg
    10681 gggccgctgt cggatcgtat tggtcgccgt ccggtgatgc tggcgggggt ggtgtggttt
    10741 atcatcacct gtctggcaat attactggcg caaaacattg aacaattcac tctgttgcgc
    10801 ttcttgcagg gcataagcct ctgtttcatt ggcgctgtgg gatacgccgc aattcaggaa
    10861 tccttcgaag aggcggtttg tatcaagatc accgcgctga tggcgaacgt ggcgctgatt
    10921 gctccgctac ttggtccgct ggtgggcgcg gcgtggatcc atgtgctgcc ctgggaaggg
    10981 atgtttgtct tgtttgccgc attggcagcg atctcctttt tcggtctgca acaagccatg
    11041 cctgaaaccg ccacgcgtat aggcgagaaa ctgtcactga aagaactcgg tcgtgactat
    11101 aagctggtgc tgaagaacgg ccgctttgtg gcgggggcgc tggcgctggg attcgttagc
    11161 ctgccattgc tggcgtggat cgcccagtcg ccgattatca tcattaccgg cgagcagttg
    11221 agcagctatg aatatggctt gctgcaagtg cctattttcg gggcgttaat tgcgggtaac
    11281 ttgctgttag cgcgtctgac ctcgcgccgc accgtacgtt cgctgattat tatgggcggc
    11341 tggccgatta ttattggtct gttggtcgct gctgcggcaa cggttatctc atcgcacgcg
    11401 tatttatgga tgaccgccgg gttaagtatt tatgctttcg gcattggtct ggcgaatgcg
    11461 ggactggtgc gattaaccct gtttgccagc gatatgagta aaggtacggt ttctgcggcg
    11521 atgggaatgc tgcaaatgct gatctttacc gtcggtattg aaatcagcaa acatgcctgg
    11581 ctgaacgggg gcaacggact gtttaatctc ttcaaccttg tcaacggaat tttgtggctg
    11641 tcgctgatgg ttatcttttt aaaagataaa cagatgggaa attctcacga agggtaaaaa
    11701 aatgcctgat tgctttgtgc gatcaggcat tctcgaatta atggtgatgg tcgtcaatct
    11761 ggtgttcgat aaccatccct tcacctacgc tggcaagatg gcgaacataa ggatgcgggc
    11821 ggtaagccgg agctggcgca ggagccacat agacggtttg cggcacagtc gcgacgctga
    11881 ccgcttgtgg aacgctgact gaatcaggaa ccacaaccac tttgtatcca ctcggcacat
    11941 cgacggtgac actttgcgcc atcgccacac cagtaaagcc cattaaaagc gcgcccaaca
    12001 gtagacaatt tttcataaac atttcgcctg aaataatgca gcgggttaat ttccgcgaat
    12061 tatgcagcct tcattcaggt attgtgttgc cgggcgtgct gccgttttta ttggtcaaat
    12121 ggcgcttcgt gtttaagcac tttatcgatc acatccagta cgccttcacg gttattggag
    12181 cctgcccggt attttgccgc tgcgacgacc gcattgccgg cattttccat tgcaaaacta
    12241 aagcctgcct gacgcagcat ctcaatatcg ttaccgccat cgccaaagac caccacttcg
    12301 ctgtcgtcta ttccccataa tttctgcagt tggcgaaggc cattggcttt atgtacgccg
    12361 gggataatca gatcgatgct gccgttgccg gtgtggaccg gcaccataat atcgccgatg
    12421 gcctcatgta atgctttttg tacttgtgga atcagttcat cggaaagatt caggccaaac
    12481 ttaaagaaga tatcctctaa gttgtcaaag ttatcgacgt attccagacg gtgataatac
    12541 atttccgcca ccgttttcat ggcatcgtca tattttttga gcgtataggc gctatttttt
    12601 ccgcaggcaa taatttccac ttccgggcgc gtcagcaaat gttccacgac agtagtaaac
    12661 gcatccttcg acagctcgcc attaaaaaca tctttgcctt cgctcactac ccagccgccg
    12721 ttttcagcca caaaggcaat ttcattcgca atttcaggga agaaagagat caactgataa
    12781 tattgattcc cgctggcgac tacaaagcga attccttgtg ctttcatttg ctgatactga
    12841 gccataaacc gctcacggtt ataggttttt tgatcgctta agaaagtacc atccatgtct
    12901 accgcaatta atttaatgct catcaactat tctccatcgc cgtctgcgtt ttggtatcgg
    12961 gtttggcgac ggctttcgcg acaatagccg cgagaataac cagcgccagt acaaccagca
    13021 ttgcactacg taatccataa tgttcgccga gatagcccag cagcggcggc ccgacgagga
    13081 aagccagata accggtcgtt gctaccacac tgacgcgggt tggtgcatcg gggccggtat
    13141 cgctggcggc agaaatggtc agcgggaagc ccagcgaggc acccagtccc cagagtacaa
    13201 cagacacccc agcgacccag gcgctatcga caaaaataat cagcccaata cccaacgccc
    13261 ccattagtgc actggcccga accacggcaa cgcgactgta acggtcgatg aaccaaccgc
    13321 cggtgaagcg tccaacggtc atccccaggg taaaaccggc ataaatcagc gagccggaag
    13381 tagggctaaa accgtgaccg tcaaccatta ataagggtaa ccagtcgttg gcagaacctt
    13441 cggcaaaggc catcgccagc accacaacac ctatcagcag caactggata tcgcgataaa
    13501 aaggaatgcc tttttcgcca tgctgggtgc catcggcagc atttttgccc gtaccgtcag
    13561 ggattgcctg aatggcgata taaataggcg cgatacctac cagcgccgcc aataaaatgt
    13621 gcaccgttgc cggaacgcca aaggccgtca gtgccatccc gacaccagcg cctgccagcg
    13681 tgcccaggct ataaaaaccg tgcatcattg gcaaaaccgt tttattcatt tctcgctcaa
    13741 cggcggcacc ttcaacgttt atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc
    13801 cgagaccaac ggcaaagagc aggggcgatg tcagccagag tgccagactt aatatcatca
    13861 tcccgatcaa tgcgcaggac atcgtgacca ggatgacatt acgcgtgcca aagcgtttca
    13921 ctaaccacgc cgagcagaga ataccgctca tcgaaccgat cgacagacca aagagaacac
    13981 cgcccatttc agcgatcgag acagagagaa tatcgcggat agcaggcgta cgggttgccc
    14041 aggacgccat taacaggcct ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt
    14101 tcaatgcatt acgtgaagaa tttacggtca tagatcacgt caaaataaga agagggaaga
    14161 caacattagc aaggttgtgt acatttgtac acaattgcag agagaggaaa tgacatgcgt
    14221 cgcgctaacg atccgcaacg gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa
    14281 ctttacggaa tacatgctgt tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg
    14341 ggatcgatga cctactattt ttcaggaatt gatgagttgt tgctggaggc gttcagcagt
    14401 tttactgaga ttatgtcccg gcaatatcag gcatttttta gcgatgttag tgatgctcaa
    14461 ggcgcatgcc aggctatcac cgatatgatc tacagctcac aggttgcaac gccggataat
    14521 atggagctga tgtaccagct ctacgcgctg gctagccgaa aaccgctatt aaaaacggta
    14581 atgcaaaact ggatgcagcg cagtcagcaa acgctcgaac aatggtttga acccggaacc
    14641 gcccgcgcgc ttgatgcgtt tattgagggg atgacgctgc attttgtcac cgaccgtaag
    14701 ccgctatcgc gcgaggagat tttgaggatg gttgagaggg ttgcagggta gtagataagt
    14761 tttagataac aaaaaaccca tcaaccttga accgaaatgg cggggttgat gggctccaca
    14821 aaatggggac atcaaagaaa agcagtggca ctaattaaga ctgatgccct gcggaaaagt
    14881 tctgcggttg tgcaaaaaaa tttcattttc agggcaactt cagttttatc ctaatcctgg
    14941 ccataccatg acgatgattg tccctgccag cgtcagcagg acgttggcga ttgcgtaggt
    15001 gcccgcgtag cccagcgcag ggatgttact gcgagctgta tcactgatga tctccattgc
    15061 cggcgcgcag gtgcgtgcgc ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa
    15121 tacataagca ccgaacaaga aacagataac cacgggcaca agactgacaa ttaatccggc
    15181 aatcaacatc tgaccgccaa tcgcgcccag gccgttatta ataccgctac cggcgctcag
    15241 accaacgcct gccataaaca ccatcaagcc gaactctttc accatgctta atgcaccttg
    15301 cggaatgtaa ccgaaggtcg ggtggttagc acgcataaag cccagcataa ttccggcgaa
    15361 taacaacccg gcagcgttcc ccatgccgaa actgaatgtg ctgaactgga aggtgatcat
    15421 cccgatcatc agcccaataa caaagaaggc gcaaaatgcc agcaggtcag tgacctggct
    15481 gtgaatcgag ataaagccga tgcgatcggc gatggttttt acgcgacggg catcaccgct
    15541 gacttgtaaa acgtcacctt tgttaagcac gacgttgtca tctatcggca tctcaatctg
    15601 gctacgaatg acgcggttaa ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg
    15661 tttacctaca gcgttatggt ttttaacgac cacttcttca gtgacgatac gcatgtcgag
    15721 aaggtcacga tcgaaaactt ctttaccgtt acggaagctg ggatcgagtc gggcatgggc
    15781 gtcgggatag cctaccaacg ctatttcatc gcccatttgt agcacggcat caccgtctgg
    15841 atttgccaga atcccgttac gtcgaatacg ttcaatgtag cagccggttt gtcgataaat
    15901 acccagttca cgcagatttt tgccgtcggt ccaggccacc agctccgggc cgacgcgata
    15961 ggcgcggatc accggtaaat aaaccttacg gttggcatca gtgtccaggc cacgttcgcg
    16021 ggcgatttgc tgggcgctgg tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc
    16081 accaacaatc aaactcacca gaccgattaa ataggttaag gcatacccga ggctcagatt
    16141 atccagtgcc agtgagagct gcctgctttc catgccggaa tgacgcagtg tatcgccagc
    16201 accgaccaga accggtgtcg acgtcataga gcctgctaac ataccggccg tcaggccaat
    16261 atcccagcca aacagcttac ctaaccctaa ggcgatcacc agcgcactgc caaccatcac
    16321 cagtgctaac attaggtaat ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc
    16381 ggcttcgacc ccgacgcaga aaataaacag cataaagcca agattaagcg catcggtgtt
    16441 aatgctgaaa tgttgttggc ctaataacag cgatacgact aaaacgccaa tggaattacc
    16501 cagttggatc gaaccaagtc gtaactttcc gagacatagc ccaagcgcga ggaccacaaa
    16561 taataacaga atgtaattcc catttaacaa ttcggcgacg tttatattca cggaggctaa
    16621 cttcttgttt actagtaagc tgttgaaaga aatggtaatt tacgataatg ttttttacca
    16681 gaattcaggg cgcagattca ttcagcgcac ctaaacgata gtaaagtaac aatatatttt
    16741 actagtgtaa tcacattagg tatcaacggc tatatgaatt gcgttggcct atattagcat
    16801 ggaatgcgaa gcggctttat cttactgaac gccacactgg cgaaaaatgt gttcgataga
    16861 cgcagtgtca ggaggaacga gtgaaacata aacaacgttg ggcgggggca atctgctgtt
    16921 ttgtcctctt cattgtggtg tgcctttttc tggcgacgca catgaaaggc gcttttcggg
    16981 ctgccgggca tcctgaaatc ggcttgctat ttttcattct tcctggagca gttgccagtt
    17041 tcttttcaca gcgtagagaa gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct
    17101 gttcaatgct cattatgcgg ctgttttttt caccgacgcg ctcattctgg caagagctgg
    17161 catggttact aagcgcggtg ttctggtgtg cgctgggggc actgtgtttc ttatttatca
    17221 gtagtttgtt taaaccacag cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt
    17281 cagacgatca ggcgtccaga ttttctttca cccatgcagc aaaatcggta tagccgccga
    17341 tatgttgctg atcgacaaaa atctgcggca cggtttctac gggtttacct gccttttgtt
    17401 gtagatcttc tttagtgatc ccttccgcac gaatatctac atactgatac tgaaaatcat
    17461 cgcgttcatt gctcaatttc tcagccagat cttttgcacg cacacagtaa gggcaacccg
    17521 aacgaccaaa aataacggtt tgcattattt ctctcctcat agatttatgc ctgtaatgat
    17581 cacgctaaaa tgtattcgct gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta
    17641 taactgttaa acacaataca gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa
    17701 gcgtatgcgc gcgatcggta aattgcctaa aggcgtgttg atactggaat ttatcggaat
    17761 gatgctactg gcggtggcgc tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt
    17821 ttctcggcca gaagtgcaga ttctgatgat ttttctcggt gttttgctca tgcttcccgc
    17881 tgcggtggtg gttattcttc aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc
    17941 accgcaatat tcacgttcag aaagagaaaa agataatgac gccaaccatt gaacttattt
    18001 gtggccatcg ctccattcgc catttcactg atgaacccat ttccgaagcg cagcgtgagg
    18061 cgattattaa cagcgcccgt gcgacgtcca gttccagttt tttgcagtgc agtagcatta
    18121 ttcgcattac cgacaaagcg ttacgtgaag agctggtgac gctgaccggc gggcaaaaac
    18181 acgtagcgca agcggcggag ttctgggtgt tctgtgccga ctttaaccgc catttacaga
    18241 tctgtccgga tgctcagctc ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg
    18301 caatgatggc gcagaatgca ttaatcgcag cggaatcgct gggattgggc ggggtatata
    18361 tcggcggcct gcgcaataat attgaagcgg tgacgaaact gcttaaatta ccgcagcatg
    18421 ttctgccgct gtttgggctg tgccttggct ggcctgcgga taatccggat cttaagccgc
    18481 gtttaccggc ctccattttg gtgcatgaaa acagctatca accgctggat aaaggcgcac
    18541 tggcgcagta tgacgagcaa ctggcggaat attacctcac ccgtggcagc aataatcgcc
    18601 gggatacctg gagcgatcat atccgccgaa caatcattaa agaaagccgc ccatttattc
    18661 tggattattt gcacaaacag ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat
    18721 acgcgggctt ttgaccaggt ctgacagaga ggtgcagggt gaaaattgcc atattgtccc
    18781 gggatggaac gctctattcg tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc
    18841 tggttgaaat tcttgatccg ctttcttgct acatgaacat aaatcctgcg gcgtcttcta
    18901 ttcactacaa aggccgcaag ttaccccatt ttgacgcagt gatcccgcgt attggcaccg
    18961 ccattacctt ttatgggacg gcggcactgc gccagttcga gatgctgggg agctatccgc
    19021 tcaatgagtc ggtcgccatt gcccgggcgc gtgacaaatt gcgttccatg caactgctgg
    19081 cgcgtcaggg catcgacctg cctgtcacgg gcattgcgca ttcgccggat gataccagcg
    19141 atttaatcga catggtcggt ggtgcgccgc tggtggtcaa gctggttgaa ggcacgcagg
    19201 gaattggcgt cgtgctggcg gagacgcgtc aggcggcgga aagcgtgatt gacgctttcc
    19261 gcggtctgaa cgcgcatatt ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata
    19321 tccgctgtct ggttgttggc gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg
    19381 gcgattttcg ttccaatttg catcgtggcg gcgcggcaag cgtcgccagt atcacaccac
    19441 aggagcgtga aatcgcgata aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg
    19501 atattctgcg tgctaatcgc gggccgttgg tgatggaggt gaatgcgtcg ccggggctgg
    19561 aaggaataga aaaaaccacc ggtatcgaca tcgcgggtaa aatgatccgc tggatcgaac
    19621 gccacgctac gacagaatat tgcctgaaaa cgggtggtta gtcgcaatca cattactgat
    19681 catggttttg cctgcgcttt ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt
    19741 acaaaattat gacatcgctg gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg
    19801 acctggggat gagttttttt gaatgtgata actgtcaggc tctgcatctg ccccatatgc
    19861 agaatttcga cggtgtcttt gatgccaaaa tcgatctgat cgataacacg atcctgtttt
    19921 ctgccatggc ggaagtccga ccttcagccg tattgccgct ggcggcggat ttatctgcca
    19981 tcaatgccag ttcgctgacc gtgaaagcat ttcttgatat gcaggatgat aatctgccaa
    20041 agctggtggt ttgccagtct ttatccgtta tgcagggcgt aacctatgag cagtttgcat
    20101 ggttcgtgcg tcagagcgaa gagcagattt cgatggtcat tcttgaagct aatgcccatc
    20161 aactgctgtt accgactgat gatgaagggc aaaacaacgt taccgaaaac tatttcctcc
    20221 actgataact cctttcgtgc acgcagtcgc tggtgcagtg gctgcgcgct gcaaaattat
    20281 ctgctgtttt taaccttttc ttaaagatta tttcacttct cttgtgtcga tttggcttta
    20341 tcacatagag caaatacgca taaaaatttg ttaaataccg ttttttaatc cgagctatag
    20401 tctcaaaccc tggctaaagt tattcttgcg atgcttttat atagcgagca gtgctggccg
    20461 ggagagagtt ctcttttctt acaccgcgcc gataaaaaat atgcacgttt attgcatatc
    20521 tttcagtgtg acaacttttg ttcgtttgtt aacgaacttt cagaaggaaa gagatatgac
    20581 cgccttaaat aaaaaatggc tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt
    20641 cggcacgctc gcggctgaac aaaaaacact ccacatttat aactggtctg attatatcgc
    20701 cccggacacg gtggccaatt ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt
    20761 cgactctaac gaagtactgg aaggcaaatt aatggccggg agcaccggct ttgacctggt
    20821 ggtgccgtca gcaagctttc ttgaacgcca gctgacagcg ggagttttcc agccgctgga
    20881 taaaagcaaa ttgccggagt ggaagaatct cgacccggaa ctgctgaagt tggtcgccaa
    20941 acacgatccc gacaataaat ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta
    21001 taacgttgat aaagttaaag cggtgctggg cgaaaacgcg cccgtcgata gctggaactt
    21061 gatcctcaag cctgaaaatc tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc
    21121 gccagaagaa gtttttgcta ccgtgctgaa ttatctcggc aaagatccca acagcactaa
    21181 agcggatgat tacaccggac cggctaccga tctgctgtta aagctgcgcc cgaacattcg
    21241 ttatttccat tcatctcaat acattaacga cctggcaaac ggcgatatct gcgtcgctat
    21301 tggctgggcg ggcgatgtct ggcaggcgtc aaaccgcgcg aaggaagcga agaatggcgt
    21361 taatgtctcg ttctcgattc caaaagaagg ggcgatggcg ttctttgatg tcttcgccat
    21421 gcctgcggat gccaaaaaca aagacgaagc ctatcagttc ctgaattacc tgctgcgccc
    21481 ggatgtagta gcgcatattt ccgaccatgt gttctatgcc aacgccaata aagcagccac
    21541 gccgctggtg agtgcggaag tccgtgataa cccgggtatt tatccgcctg cggatgtccg
    21601 tgcgaagctg ttcactctga aagtgcagga tccgaaaatc gaccgtgtgc gcacccgcgc
    21661 gtggaccaaa gtgaagagcg gaaaataatc cgcagcctta gatgccgggc gggcgcacca
    21721 cacccgccgg caattcgcac catcatggtg cgcttgcaca cattcaatgc cggagagcag
    21781 ccgtgaatga cgctatccct cgcccgcagg cgaaaacccg taaggcgctg acgccgctat
    21841 tagaaatccg caacctcacc aaatcctacg atggtcaaca tgcggtggat gatgtcagcc
    21901 tgactatcta caaaggtgaa atcttcgcgc tgctgggcgc ttccggctgt ggtaaatcca
    21961 cgctgctgcg tatgctagca ggttttgaac aaccctctgc cggacagatt atgcttgatg
    22021 gcgtcgattt gtcacaggtg ccaccttatc tacgccccat caatatgatg tttcagtctt
    22081 acgcattgtt tccccatatg actgtggagc agaacatcgc ctttggcctg aaacaggaca
    22141 aactaccgaa agcggaaatt gccacccggg tcaacgagat gcttgggctg gtgcacatgc
    22201 aggagttcgc caaacgcaaa ccgcatcagc tttccggtgg tcagcgacag cgtgtggccc
    22261 tggcccgaag ccttgccaaa cgcccgaaac tattactgct cgatgagccg atgggcgcgc
    22321 tggataaaaa gctgcgtgac cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg
    22381 gtgtgacttg tgtgatggtc acccacgatc aggaagaggc gatgaccatg gcggggcgta
    22441 tcgccattat gaatcgcggg aaatttgtcc agattggcga gccggaagag atctacgagc
    22501 atccaactac ccgctacagc gctgaattta tcggttcggt taatgtcttt gaaggtgtac
    22561 tcaaagagcg tcaggaagat ggcctggtgc ttgattcgcc ggggctggtg catccactga
    22621 aagtcgatgc ggatgcttca gtggtcgata acgtgccggt acacgtagcg ctgcgcccgg
    22681 aaaaaatcat gctttgcgaa gagccgcccg ccaatggttg taacttcgcg gtgggggagg
    22741 tgatacacat tgcctatctc ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc
    22801 agatgattag cgcccagcta caaaacgccc atcgtcatcg taaagggtta ccgacctggg
    22861 gcgacgaagt gcgtttgtgc tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag
    22921 caaagatgaa tacacttgaa cctgctgccc agtcgaaacc gccgggcgga tttaagctgt
    22981 ggttgtcgca gctgcaaatg aagcatgggc gcaaactggt cattgcgttg ccatatatct
    23041 ggttgatctt gctgtttctg ctgccatttc tgattgtctt taaaataagc ctggcggaga
    23101 tggcgcgcgc tattccacct tataccgaac tgatggagtg ggctgacggg caactgtcca
    23161 tcactcttaa tctcggtaat tttctgcaac tgaccgacga tccgctctat ttcgatgctt
    23221 atctccagtc gctacaggtg gctgcgattt cgacattttg ctgtttactg atcggctatc
    23281 cgctggcgtg ggcggtggcg cacagtaagc cttcgacccg taatatttta ttactactgg
    23341 tgatcctgcc gtcgtggacc tcgtttctga tccgcgttta tgcctggatg ggaatattaa
    23401 aaaacaacgg tgtgctgaat aattttctgc tgtggctggg ggttatcgat caaccgctga
    23461 ccattcttca taccaatctg gcagtttata ttggcattgt ttacgcctac gtgccgttta
    23521 tggtactgcc gatttatacc gcgttgattc gtattgatta ttcgctggtg gaagcagcgc
    23581 tggatctcgg tgcacgaccg ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag
    23641 gtgggattat tgccggatcg atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc
    23701 cggaactgct cggtggcccg gacagcatca tgatcgggcg cgtgctatgg caagagtttt
    23761 tcaataaccg cgactggccg gtggcctcgg cggtagcgat catcatgttg ctgctgctaa
    23821 ttgtgccgat aatgtggttc cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa
    23881 taatttaccg gtagttcgtt cgccctggcg gattgtgatt ttgctgctgg gctttacctt
    23941 tctctacgcg ccaatgctga tgctggttat ctattcgttt aacagctcga agctggtgac
    24001 ggtgtgggcc ggctggtcaa cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat
    24061 gagtgcggtt ggtttaagcc tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct
    24121 cgggactatt gcggcggtgg tgctggtgcg ctttggcaga tttcgcggat caaatggctt
    24181 tgcctttatg atcaccgcgc cgctggtgat gccagatgtc atcacgggct tgtcgctgtt
    24241 gttattattc gtcgcgcttg ctcatgccat tggctggcct gcggaccgcg gtatgctcac
    24301 catctggctg gcgcatgtca ctttttgtac ggcttatgtg gcggtcgtta tttcttcaag
    24361 attgcgggaa ctggatcgct cgatagaaga agcagcgatg gatctcggtg cgacgccgct
    24421 gaaagtgttt ttcgtcatta cgctaccgat gatcatgccc gcgatcattt ctggctggtt
    24481 actggctttt actttgtcgc ttgatgatct ggtgatcgcc agctttgtat ccgggccggg
    24541 agccaccacg ttaccgatgc tggtcttttc cagcgtgcgg atgggggtga atccggaaat
    24601 caacgccctt gcaacgttaa ttctcggtgc ggtcggaatt gtcggattta tcgcctggta
    24661 tctgatggct cgcgcagaaa aacagcggat acgcgatatc cagcgtgcaa gacatggctg
    24721 aagacactaa aatttgccaa cctggctaca taatgccgcg catgccgcgg cattgttttc
    24781 atggaagacg aaacgttggg attttttaag aaaacatctt catctcatgc tcgcctgaat
    24841 gtgcctgcgc tggtgcaggt ggcggcgctc gccattatta tgatccgtgg cctcgacgtg
    24901 ctgatgattt tcaatacgct gggcgtgcgc ggtattggcg agttcattca tcgcagcgta
    24961 caaacctgga gtttaacgct ggtcttttta agcagtctgg tgctggtttt cattgagatc
    25021 tggtgtgcgt tttcactggt gaaagggcgt cgctgggcgc gctggctata tctgctgaca
    25081 caaatcaccg ccgcaagtta cttgtgggcg gcttcgctgg ggtatggtta tccggagctg
    25141 ttcagcattc ccggtgaatc aaaacgtgaa atcttccata gcctgatgct gcagaagctg
    25201 ccggatatgc tcatcctgat gctgctgttc gttccctcga ccagtcggcg gttcttccag
    25261 ttgcaataat gtgtataatc gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc
    25321 actttacgac gcgggtcgct gtcgttcctg tcagtggata acgcagccga ttccagagca
    25381 actctccgct aaaaccgccg atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg
    25441 gtgcgcgccg gtgtcaggcc cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag
    25501 tggtagcgtt gaaaaaccac tgctcggtat gctgcatcgc gatggcacac cagaagacct
    25561 ttgtgactgc ccgctttatc cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt
    25621 tatcgcccga gcggggttaa cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata
    25681 cattctgctg actgaaagcc agagtgatgg cggcatgatg ctgcgctttg tactgcgttc
    25741 tgataccaag ctggcgcaac tgcgtaaggc gctgccgtgg ttacaggaac aactaccgca
    25801 gctgaaagtt attaccgtca atattcagcc ggtacatatg gcgattatgg aaggggagac
    25861 ggagatctac ctgaccgaac aacaggcact ggcggagcgt tttaatgacg taccgctgtg
    25921 gatccgtccg caaagtttct tccagactaa tccggcggtc gccagccagt tgtacgccac
    25981 cgcgcgcgac tgggtacgac agctgccggt taaacatatg tgggatctgt tctgcggtgt
    26041 ggggggcttt ggtttacact gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc
    26101 gccagaggcc attgcctgtg caaagcagtc agccgctgaa ctgggcttaa cgcgtctgca
    26161 atttcaggcg ctggactcca ctcagtttgc caccgctcag ggggaggtgc cggagctggt
    26221 gctggttaat ccgccgcgcc gcggcattgg taaaccgcta tgtgattatc tctcaacaat
    26281 ggcaccgcgt tttattatct actccagctg taacgcccaa accatggcga aagatatccg
    26341 cgaactgcca ggttaccgca tcgaacgggt acagcttttt gatatgttcc cgcacaccgc
    26401 gcactatgaa gtgatgacgc tgctggtgaa gcaataaaaa agccgcaggt gcggcttcag
    26461 attgctgatc aggtgcgctt tgttcatgcc ggatgcggcg tgtacgcctt atccggccta
    26521 caaaatcatg ctaaatcaat acattgcaga gttgatgtgg gccagataag cgcagcgcat
    26581 caggcaatct tacttttgtc atcagtctca attactgcgg gaaccactgg tcattgattt
    26641 tctgatatgt accgtcagct ttaattgctg ccagcgcgtt attcagtttt tccagcagag
    26701 ctttgttatc cggacgtaca gcgataccca ggccggtgcc gaagtattgc ggatcggtca
    26761 ctttttcagt cgcaacgccc agttgcggat tggttttcag ccattcgttt accaccgctg
    26821 tgtcaccaaa taccccatca atacgaccat ttttcagatc gataaaggca ttctgataac
    26881 tgtcatagga gacagttttc acttcggggt gctgatcctg aatatatttc tggtgcgtgg
    26941 tgccgttttc catcccaata cgtttgcctt tcagatcggc aaacgttttg taggtatctt
    27001 ttttggcaat cacgacggct gagttttcat agtagggcgt ggtaaacgat acctgcttgc
    27061 tacgctcggg cgtgatatcc ataccggaga ttacggcgtc atattttctg aatttcagtg
    27121 acgggatcag gctgtcgaac gcgtgattag taaaagtaca ttctgcctgc atttgtttgc
    27181 acaaggcttt tgccagatcg atatcaaagc cgacaatctc attattagca cctatagatt
    27241 caaagggtgg ataggtggct gaaacgccaa aattgatttt ctctgcggca gaagcaccga
    27301 aagtaaagga agcaagtaaa gcggcaagaa ctaacttttt catgatggaa ctcccgtctg
    27361 tcaatcttat gatttttggc cgtgtctgcg gcatgggata acaa
//
LOCUS       DAPGWD010000051        27312 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPGWD010000051 DAPGWD010000000
VERSION     DAPGWD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.051"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            376..2799
                     /gene="pgaA"
                     /locus_tag="RHX88_002096"
     CDS             376..2799
                     /gene="pgaA"
                     /locus_tag="RHX88_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDV2230785.1"
                     /translation="MYSSSRKRCPQTKWALKILTAAFLAVSPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITIYNSYRHQQLPARGYA
                     AVAIAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALQNNQLVAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQIDHLGALLTRDRYKDVISHYQRLKKTGQIIPLWAQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDLSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2808..4826
                     /gene="pgaB"
                     /locus_tag="RHX88_002097"
     CDS             2808..4826
                     /gene="pgaB"
                     /locus_tag="RHX88_002097"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDV2230786.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSPLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSSQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4819..6144
                     /gene="pgaC"
                     /locus_tag="RHX88_002098"
     CDS             4819..6144
                     /gene="pgaC"
                     /locus_tag="RHX88_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDV2230787.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6146..6559
                     /gene="pgaD"
                     /locus_tag="RHX88_002099"
     CDS             6146..6559
                     /gene="pgaD"
                     /locus_tag="RHX88_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDV2230788.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6609..7397)
                     /gene="phoH"
                     /locus_tag="RHX88_002100"
     CDS             complement(6609..7397)
                     /gene="phoH"
                     /locus_tag="RHX88_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDV2230789.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8016..9287)
                     /gene="efeB"
                     /locus_tag="RHX88_002101"
     CDS             complement(8016..9287)
                     /gene="efeB"
                     /locus_tag="RHX88_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDV2230790.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9293..10420)
                     /gene="efeO"
                     /locus_tag="RHX88_002102"
     CDS             complement(9293..10420)
                     /gene="efeO"
                     /locus_tag="RHX88_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDV2230791.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10478..11308)
                     /locus_tag="RHX88_002103"
     CDS             complement(10478..11308)
                     /locus_tag="RHX88_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDV2230792.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11850..13358)
                     /gene="putP"
                     /locus_tag="RHX88_002104"
     CDS             complement(11850..13358)
                     /gene="putP"
                     /locus_tag="RHX88_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDV2230793.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13517..13726)
                     /locus_tag="RHX88_002105"
     CDS             complement(13517..13726)
                     /locus_tag="RHX88_002105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230794.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13781..17743
                     /gene="putA"
                     /locus_tag="RHX88_002106"
     CDS             13781..17743
                     /gene="putA"
                     /locus_tag="RHX88_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV2230795.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEAILSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLCGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17783..18421)
                     /gene="rutR"
                     /locus_tag="RHX88_002107"
     CDS             complement(17783..18421)
                     /gene="rutR"
                     /locus_tag="RHX88_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDV2230796.1"
                     /translation="MTQGAVKTKGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            18709..19800
                     /gene="rutA"
                     /locus_tag="RHX88_002108"
     CDS             18709..19800
                     /gene="rutA"
                     /locus_tag="RHX88_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDV2230797.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19800..20492
                     /gene="rutB"
                     /locus_tag="RHX88_002109"
     CDS             19800..20492
                     /gene="rutB"
                     /locus_tag="RHX88_002109"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDV2230798.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20504..20890
                     /gene="rutC"
                     /locus_tag="RHX88_002110"
     CDS             20504..20890
                     /gene="rutC"
                     /locus_tag="RHX88_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDV2230799.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20898..21698
                     /gene="rutD"
                     /locus_tag="RHX88_002111"
     CDS             20898..21698
                     /gene="rutD"
                     /locus_tag="RHX88_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDV2230800.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21708..22298
                     /locus_tag="RHX88_002112"
     CDS             21708..22298
                     /locus_tag="RHX88_002112"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDV2230801.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            22309..22803
                     /gene="rutF"
                     /locus_tag="RHX88_002113"
     CDS             22309..22803
                     /gene="rutF"
                     /locus_tag="RHX88_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDV2230802.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22824..24152
                     /gene="rutG"
                     /locus_tag="RHX88_002114"
     CDS             22824..24152
                     /gene="rutG"
                     /locus_tag="RHX88_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDV2230803.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(24235..24408)
                     /locus_tag="RHX88_002115"
     CDS             complement(24235..24408)
                     /locus_tag="RHX88_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV2230804.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24781..25377
                     /gene="wrbA"
                     /locus_tag="RHX88_002116"
     CDS             24781..25377
                     /gene="wrbA"
                     /locus_tag="RHX88_002116"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV2230805.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25398..25625
                     /locus_tag="RHX88_002117"
     CDS             25398..25625
                     /locus_tag="RHX88_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDV2230806.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25663..26904)
                     /gene="agp"
                     /locus_tag="RHX88_002118"
     CDS             complement(25663..26904)
                     /gene="agp"
                     /locus_tag="RHX88_002118"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDV2230807.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
BASE COUNT         6679 a         7062 c         6909 g         6662 t
ORIGIN      
        1 ttaattcata gatacattag gaataacaat taaatccgtg agtgccgtag cgcagccttt
       61 catcaggact ttcgaaaaat ccgaaatcat gcatcggaat ttactgattt aattatttta
      121 ctcctaactt attttgaaaa aggcattggg aattatgccg tattcctgaa gatcctcatc
      181 attggaatgg attttcgggc gagaaaagga ttttatatgg actctctgct catcatttct
      241 tcttctcatc atcaacaatt cacgtctctc ttccgcgttt aataacgggt tatgaggtgc
      301 aaaaaatatc tttcttttca gttacctgtc attagataca gagagagatt ttggcaatac
      361 atggagtaat acaggatgta ttcaagtagc agaaaaaggt gcccgcaaac caaatgggct
      421 ttgaaaattc ttactgccgc atttttagca gtgagtcccg cggcgaagag tgccgttaat
      481 aacgcctatg atgcattgat tattgaagct cgcaagggaa atactcagcc agctttgtta
      541 tggtttgcac aaaaatcagc actcagtaat aaccaaattg ctgactggtt acagattgcc
      601 ttatgggccg ggcaagataa acaggttatt accatttaca acagctaccg tcatcagcaa
      661 ttaccagcgc gtggttatgc agctgtcgcc atcgcttatc gtaacctgca acaatggcaa
      721 aattcactta cactgtggca aaaggcgctc tctctggagc cgcaaaataa ggattatcaa
      781 cggggacaaa ttttaaccct ggcagatgcg ggtcactatg atactgcgct ggttaaactt
      841 aagcagctta actctggagc accggacaaa gccaatttac tcgcagaagc ctatatctat
      901 aaactggcgg ggcgtcatca ggatgaatta cgggcgatga cagagtcatt acctgaaaat
      961 gcctctacgc aacaatatcc cacagaatac gtgcaggcat tacagaataa tcaacttgtt
     1021 gccgcgattg acgatgccaa tttaacgcca gatattcgcg ctgatattca tgccgaactg
     1081 gtcagactgt cgtttatgcc tacgcgcagt gaaagtgaac gttatgccat tgccgatcgc
     1141 gccctcgccc aatacgctgc attagaaatt ctgtggcacg ataacccaga ccgcactgcc
     1201 cagtaccagc gtattcagat tgatcatctt ggcgcgttat taactcgcga tcgttataaa
     1261 gacgttattt ctcactatca gcgtttaaaa aagacggggc aaattattcc gctctgggcg
     1321 caatattggg ttgcgtcggc ttatctcaaa gatcatcagc cgaaaaaagc acagtcaata
     1381 atgaccgagc tcttttatca caaggagacc attgccccgg atttatccga tgaagaactt
     1441 gcggatctct tttacagcca cctggagagt gaaaattatc ccggcgcgct aactgtcacc
     1501 caacatacca ttaatacttc gccgcctttc ctgcggttaa tgggcacgcc tacgagcatc
     1561 ccgaatgata cctggttaca ggggcattcg tttctctcaa ccgtagcaaa atatagtaat
     1621 gatcttcctc aggctgaaat gacagccaga gagcttgctt ataacgcacc aggaaatcag
     1681 ggactgcgca ttgattacgc gagtgtgtta caagcccgcg gttggcctcg tgcagcagaa
     1741 aatgaattaa aaaaagcaga agtgatcgag ccacgtaata ttaatctgga ggttgaacaa
     1801 gcctggacag cattaacgtt acaagaatgg cagcaggcag ctgtcttaac gcacgatgtt
     1861 gtcgaacgtg aaccgcaaga tcccggcgtt gtacgattaa aacgtgcggt tgatgtacat
     1921 aatcttgcag agcttcgtat cgctggctca acaggaattg atgccgaagg cccggatagt
     1981 ggtaaacatg atgtcgactt aaccaccatc gtttattcac caccgctgaa ggataactgg
     2041 cgcggttttg ctggattcgg ttatgccgat ggacaattta gcgaaggaaa agggattgtt
     2101 cgcgactggc ttgcgggtgt tgagtggcgg tcacgtaata tctggctcga ggcagagtac
     2161 gctgaacgcg ttttcaatca tgagcataaa cccggcgcgc gcctgtctgg ctggtatgat
     2221 tttaatgata actggcgtat tggttcgcaa ctggaacgcc tctctcaccg cgttccatta
     2281 cgggcaatga aaaatggtgt tacaggcaac agtgctcagg cttatgttcg ctggtatcaa
     2341 aatgagcggc gtaagtacgg tgtctcctgg gctttcactg atttatccga cagtaaccag
     2401 cgtcatgaag tctcacttga gggtcaggaa cgcatctggt cttcaccata tttgattgtc
     2461 gatttcctac ccagtctgta ttacgaacaa aatacagaac acgatacccc atactacaac
     2521 cctataaaaa cgttcgatat tgttccggca tttgaggcaa gccatttgtt atggcgaagc
     2581 tatgaaaata gctgggagca aatattcagc gcaggtgttg gtgcctcctg gcaaaaacat
     2641 tatggcacgg atgtcgtcac ccaactcggc tacgggcaac gcattagttg gaatgacgtg
     2701 attgatgctg gcgcaacgct acgctgggaa aaacgacctt atgacggtga cagagaacac
     2761 aacttatacg ttgaattcga tatgacattc agattttaag gataaatatg ttacgtaatg
     2821 gaaataaata tctcctgatg ctggtgagta taattatgct caccgcgtgc attagccagt
     2881 caagaacatc atttataccg ccacaggatc gcgaatcttt actcgccgag caaccgtggc
     2941 cgcataatgg ttttgtggcg atttcatggc ataacgttga agacgaagct gccgaccagc
     3001 gttttatgtc agtgcggaca tcagcactgc gtgaacaatt tgcctggctg cgcgagaacg
     3061 gttatcaacc ggtcagtatt gctcaaattc gtgaagcaca tcgaggagga aaaccgctac
     3121 cggaaaaagc tgtagtgctg acttttgatg acggctacca gagtttttat acccgcgtct
     3181 tcccaattct tcaggccttc cagtggcctg ctgtatgggc ccccgtcggc agttgggtcg
     3241 atacgccagc ggataaacaa gtaaaatttg gcgatgagtt ggtcgatcga gaatattttg
     3301 ccacgtggca acaagtgcga gaagttgcgc gttccccgct cgttgaggtc gcttctcata
     3361 catggaattc tcactacggt attcaggcta atgccaccgg cagcttattg cctgtatatg
     3421 taaatcgtgc atattttact gaccacgcac ggtatgaaac cgcagcagaa taccgggaaa
     3481 gaattcgttt ggatgctgta aaaatgacag aatacctgcg tacaaaggct aaggtaaatc
     3541 cacacgtttt tgtttggcct tatggcgaag cgaatggcat agcgatagag gaattaaaaa
     3601 aactcggtta tgacatgttc ttcacccttg aatcaggttt ggcaaatgcg tcacaattgg
     3661 attccattcc gcgagtatta atcgccaata atccctcatt aaaagagttt gcccagcaaa
     3721 ttattaccgt acaggaaaaa tcatcacaac ggataatgca tatcgatctt gattacgttt
     3781 atgacgaaaa ccgccagcaa atggatcgca atattgatgt gctaattcag cgggtgaaag
     3841 atatgcaaat atcaaccgtg tatttgcagg catttgctga tcccgatggt gatgggctgg
     3901 tcaaagaggt ctggtttcca aatcgtttgc taccaatgaa agcagatatt tttagtcggg
     3961 ttgcctggca attacgtacc cgctcaggtg taaacatcta tgcgtggatg ccggtattaa
     4021 gctgggattt agatcccaca ttaacgcgag taaaatactt accaacaggg gagaaaaaag
     4081 cacaaattca tcctgaacaa tatcaccgtc tctctccttt cgatgacaga gtcagagcac
     4141 aagttggcat gttatatgaa gatcttgccg gacatgctgc ttttgatggc atattgttcc
     4201 acgatgatgc tttgctttca gattatgaag atgccagtgc accggctatc acggcttatc
     4261 agcaagcagg ctttagcggg agtctgagcg aaattcgaca aaacccggag caatttaaac
     4321 agtgggcccg ctttaaaagt cgtgcgttaa ctgacttcac tttagaactt agtgcgcgcg
     4381 taaaagccat tcgcggtcca catattaaaa ctgcacgaaa tatttttgca cttccggtaa
     4441 tacaacctga aagtgaagcc tggtttgcac agaattatgc tgatttccta aaaagctatg
     4501 actggaccgc tattatggct atgccttatc tggaaggtgt cgcagaaaaa tcggctgacc
     4561 aatggttaat acaattgacc aatcaaatta aaaacatccc tcaggctaaa gacaaatcta
     4621 ttttagaatt acaggcacaa aactggcaga aaaatggtca gcatcaggct atttcttcgc
     4681 aacaactcgc tcactggatg agcctattac aactgaatgg agtgaaaaac tatggttatt
     4741 atcccgacaa ttttctgcat aaccaacctg aaatagacct tattcgtcct gagttttcaa
     4801 cagcctggta tccgaaaaat gattaatcgc atcgtatcgt tttttatatt atgtctggtg
     4861 ttatgcatac ccctatgcgt agcgtacttt cactctggtg aactgatgat gaggttcgtt
     4921 ttcttctggc cgttttttat gtccattatg tggattgttg gcggcgtcta tttctgggtc
     4981 tatcgtgaac gccactggcc gtggggagaa aacgcaccag ctccccagtt gaaagataat
     5041 ccgtctatct ccattatcat tccctgtttt aatgaggaga aaaacgttga ggaaaccata
     5101 cacgccgctt tagcacagcg ttatgagaac attgaagtta ttgccgtaaa tgacggttca
     5161 acagataaaa cccgtgccat cctggatcgc atggctgcac aaattcccca tttgcgggtc
     5221 attcatctgg cgcaaaacca ggggaaagcc attgcgctta aaaccggagc tgccgcggcg
     5281 aaaagtgaat atctggtgtg cattgatggc gatgcgttat tagaccgcga tgcggcggca
     5341 tatattgtgg aaccgatgtt gtacaacccg cgtgtgggtg ccgtaaccgg taatcctcgt
     5401 attcgaacac gttctaccct ggtgggtaaa attcaggttg gcgagtattc ctcaattatt
     5461 ggtttgatca agcgaaccca gcgtatctat ggaaacgtat ttaccgtttc cggtgttatt
     5521 gccgcatttc gtcgcagcgc cctggcagaa gtgggttact ggagtgacga tatgatcacc
     5581 gaagatattg atattagctg gaagctgcag ttgaatcagt ggacgatttt ttacgagcca
     5641 cgggcactgt gctggatatt aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg
     5701 cgctgggctc agggcggtgc agaagtattc ctcaaaaata tgacaaggct atggcgcaaa
     5761 gaaaactttc gaatgtggcc actgtttttt gaatactgtc tgacgacaat atgggccttt
     5821 acctgcctgg tcggtttcat tatttacgca gtccaacttg ccggtgtacc gttaaatatt
     5881 gaattgacac atatcgctgc gacacatact gccggaatat tattgtgtac gttatgttta
     5941 ctgcaattta ttgtcagcct gatgatcgag aatcgctatg agcataatct gacttcatcg
     6001 cttttctgga ttatttggtt cccggttatt ttctggatgc tgagcctggc aacgacattg
     6061 gtatcattta cacgagtcat gttgatgcct aaaaagcaac gcgcccgttg ggtaagtccc
     6121 gatcgcggga ttctgagagg ttaatatgaa caatttaatt attacgaccc gacaatcgcc
     6181 agtacgttta ctggttgatt atgttgccac aaccatcttg tggacattat ttgctttgtt
     6241 catattctta ttcgccatgg atctgctgac gggttattac tggcaaagcg aggccagaag
     6301 ccgacttcag ttctattttt tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc
     6361 gctgtacaat aagctgcgtt ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc
     6421 gcaagaatat gcagagagct tagcaatacc tgatgagctc tatcagcaac tacaaaaaag
     6481 ccataggatg agcgtacact tcaccagcca ggggcaaata aaaatggttg tttcagaaaa
     6541 agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc tctgcaccga
     6601 taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg tacgcagtcc
     6661 tctttaccga accggacgat cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca
     6721 cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata
     6781 accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc agtcacattc
     6841 tgcgcctcgt caagaatgac gactgcattt tcaaaggtac gtccacgcat ataggcgaac
     6901 ggcgcaattt ccaccttccc tatttccggt cgcaggcagt actgcataaa ggaagcccct
     6961 aagcgccgga ccagcacgtc gtagaccggg cgaaaatagg gagcaaactt ttctgcgata
     7021 tctccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt gacgataatc
     7081 ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat ccaggttttt
     7141 ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc cttcagatag
     7201 tgcaattgcg cttcatttcg cgcgaggatg ggcgaagtat cgcgactgtc gcgggccata
     7261 ccaatggctt ctacgccgcc catctgcaca agcgaggtga ccgattcttc ttcacgctgc
     7321 ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cacgacgagc tttgatcact
     7381 gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca cgcgccgttg
     7441 gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc atagtttgct
     7501 tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt aacacgagta
     7561 aagagaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag aaatctccgc
     7621 aagtggtgga gcgtagctta acaagacatt gtccagaaca ggacgttacc atccgcgatc
     7681 tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa ttacaacaga
     7741 tctcgcattc attgcaacaa tatattcact tatatttaac cataaaacac catttcagtg
     7801 acattagttt ctactggaaa gatgacagag tgatgacaga tatgaaaaaa gctgtgtgct
     7861 ttcagcagga ttcgcatcag gcatctgcgc acgaacgccg gatgcggcgt gaacgcctta
     7921 tccgcctacc gaacccgcac cgattgtagg cataagaaga cgcgcaagca tcgcatcagg
     7981 catctgcgca agaacgcctt atccgtctat aaacattaaa cccgcaataa cgcgcttccg
     8041 agataatcgt tcgcgtcctt cactcccggc agcgcaaaaa aataaccgcc gccgataggt
     8101 ttaacgtatt cctccagcgc ttcgccattg agcctttttt gtacagtcag gaagcctttt
     8161 tccagatcgt gttggtaaca gacaaacagt agccccatat ccagttgccc ggagttggtg
     8221 acgcccagtg aataactgta gccacgacgc agcatcaggc tggactcact ctccgccgtg
     8281 cggggattcg ccagccggat atggctgtcc agcgcaatca ccttcccttc cggatcgctg
     8341 gcgtaatcgg gaacatcatg ctcatgctgc attcccagcg gcgcaccggt ttgcttatca
     8401 cgaccaaaaa tcgtctgctg ttctttcagc ggagttctgt cccaaaattc cactcgaaac
     8461 tgaatcaagc gtactgcctg atagctgcca ccgattgtcc acgctggctc ctgctgatct
     8521 gccgttaccc acaccacttt ttgcatcaac ttatcattct ggctatcggg attggcagtg
     8581 ccgtctttga aacccagcaa attaatcggc gtctctttgc ctttactacg cgccgcgtga
     8641 tcggaaataa acccttcccg cttccagcgc acgctgagca aatctggcgt atgtttgatg
     8701 atatcgcgca gcgcatggat caccgtgtcc tgggtgttgg cgcaaatttg tagcaacaca
     8761 tcaccatgac ataacgccgc atccagcgag tcgttgggaa aacgcgtcat cttctgcaac
     8821 tttttcggca tctgtggcgc aaggccaaag cgctcatcaa acaatgagtg acccaccgat
     8881 aacgtgatgg tgagattatc gggcgcaatg tagccgccaa gaatgccgga atcgagtggt
     8941 ggcaggcgcg gatttggcgt ttctggtgcc gctccgccct gagtcagaaa agcaaaacgc
     9001 tgagtcaaca agcgaaacaa ccgttcaaga tcgggtttat cgctggcaag cacatcaaac
     9061 gccaccagca tcattgcggc ctgttgtggc gtcaggatcc ctgcctgatg ctcaccataa
     9121 aacggctgtt tctcattgcg tgcatccggt gaaagcgtac ccggcgcact ttgcgttttt
     9181 tgtgcatgag cgaccggaca acttcccgcc agcgccagcg caccaatgcc tttcagtaaa
     9241 cgtcggcgtg acggttcatt cacgccgttt ttatcttcat actgcataat gcttaatcca
     9301 atcccagcac accgcgaagt tgcgccagat cttccgccag cgcagtaatc ggtcctttca
     9361 gtgcattccg gtcggcatcg gtcaatttgt cgtaggtttc aaaaccgtct ttagtacggt
     9421 atttcgccag aatggtatcg acctttttaa agttggcatc gacttttgcc aacagttccg
     9481 ggttggcttt ttgcagttgt ggacgcagca gatcgacaat tttctgcgag ccttcaacgt
     9541 tagcctggaa atcccacaga tcggtgtggc tgtagcgatc ttcttcaccg ctgattttgc
     9601 tggctgccac ttcctcaatc agtccggctg caccgccgac cacttttgaa ggtgggaaag
     9661 ccagttcact gatgcgtttt tgcaaatcga ccacatcggt ataaagctgg tcagcgtact
     9721 gatccatgcc tttggtggtg ttgtcgccaa acaatgcttt ttccagacgg tggaaaccgg
     9781 tgaatttcgg atcggcggct ttttgctcgt aatcatcttc acgggcgtca atgctgccat
     9841 ccagatcgga gaacagttca gcaatcggtt cgatgcgctc atagtgctgg cgcgtcggcg
     9901 catacagtgc tttcgctttt tcgatatcgc ctgctttaat agcgtcagta aaggctttcg
     9961 tgtcggtcac cagctgcgtg gtttccgcca tgacatacgc tttatatgca gtaattgcac
    10021 caccaagact taacagcgca tcactttgcg ccgcatccgc cgttgcctca cctttgacga
    10081 tcaacttccc tttcgggtta gtcagcagac cgcaggtcat atcgtattcg ccaggctgta
    10141 aattcgccgt cattttctgg ctaaagccag gggcgatatt ttcccgctct tccaccacca
    10201 tcacgccttt gaggatctcc cactccagcg ccttctggct gtggttctga ataatgaact
    10261 gtgttttccc ggcgttaacc gtaatggtca tcggttcgca ctgcttatcg gtcacggtca
    10321 ccttgacctg cggcacatta gcggcgttag ccataaaagc agaagagaac agcgcagcca
    10381 cgctcaactg caatgcgtta cggcggaagt taatggtcat gacgagacta tccctttaaa
    10441 gtatgttgta actaagcaag agtttgcgtc gtatttgtta cgcggagcga gacgctgtcg
    10501 cccctgcgcg tggtggcaga gcaaatgcca ccagcgccgg gatgagataa ataaaccaga
    10561 cggcgacttc gctgacgctc ggcgcttcct gatagccaaa aatcccttcc atcagcgtgc
    10621 caaacagcga gtgagttgag agcaccgcac tcatatcgaa ggcgatttcc tgaaagtggt
    10681 tccacaatcc ggcttcatga aatgcgcgaa tggcaccagc tgccagccct gcggcgacga
    10741 agagaataaa caggctggtc catttaaaaa atgcaccaag attgaggcga ataccgcccc
    10801 agtagagcag gaagcctagc accacggcag tagcaagacc gagcattgca cccagcggcg
    10861 gccagatccc gacatcttgt tgaaatgccg ccagcaggaa aaagaccgac tccagccctt
    10921 cccttgcaac ggcaaaaaag accatcatca ccagcgccca gccatgatga tttccacgct
    10981 gcaatgcgct atcgactgcc tgctccagtt gtactttgac gttgcgcgac actttgcgca
    11041 tccagaaaac catccaggta aggatcacca cggcgatcac cgccacgata ccttcaaaca
    11101 gttcctgctc tttttgcgga aattcgccgg tggtttcgtt aatgaagatc cccaagccca
    11161 ggcacaacgc agcggcaagc aacacgccaa tccacatcac accaatccat cggcctcgct
    11221 gggtacgctt aagatagctg gcaatcaaac tgacaatcag cgcggcttca agtccttcgc
    11281 gcaacataat gagaaacgga acaaacatgc cacccaccct taaacgtttt tctgagtcaa
    11341 tctatgcaaa gaaaagtaaa tcgcagtgat agcgattatc attacggtaa agaaaaatac
    11401 aagcggaatg tttataaaat gatgttgaaa tgtaaacaaa gagaggtaat gccaggggat
    11461 ataaacgatt attccaggta caaagatggg attgtttaca acgagccatg tgttcttgcc
    11521 ggatgccgcg taaacgccgt atccagccga caaaactgtc cagactcaat gcatcagagc
    11581 ttccatgtaa aactgataag cgggtgtatc aagcaatttc gtccatccgc gattatcaaa
    11641 tctaactttt tgcccggaac ataataacag ctcgggtgtt ggtggcgtca aaatcgctga
    11701 agcgttcccc gaaggccggg acagcagggg gcggcggcga ttggccgccc cctgcgcgct
    11761 ccctgcacca ttcaatttat atggcgaggc acattaaggg agcgcaaccc gctgctgaag
    11821 ccaaacaaaa ccgccgcagg ctaagtccct tagctttcct gcaaccgtga cggcggagcc
    11881 gaatgatagt gcgcatcggc ctcggcaaag cgtttttgca tcgccgctga cggcgcttta
    11941 cccagcaaac taaacactac aatcccgata ctgccgaaga tgaagcccgg aataatttcg
    12001 tagagtccca gccagccgaa ctgcttccag acgataaccg tcagcgcacc gatgatcatc
    12061 cccgccagcg caccgttgcg cgtcatgcgt gaccacatca ccgagaacag caccaccgga
    12121 ccaaacgccg cgccaaagcc tgcccacgcg tagctcacta agcccagcac gcggttttcc
    12181 gggtttgccg ccagcgcaat cgccaccagc gccaccacca gcaccatcac acgtcctacc
    12241 cacaccagct ctttctggct ggcctgttta cgcagaaacg ctttgtataa atcttcggta
    12301 atcgcactgg agcacaccag cagctggcag cttaacgttg acattaccgc cgccagaatt
    12361 gccgacagca gaatcccggc aatccacggg ttaaacagaa tttgcgccag ttcgataaac
    12421 acgcgctcgg cgttctggtt taccgcgcct gctaccgccg ggtgctcatt aaagtaagca
    12481 atcccaaaga agccgacagc caccgcccct gccaggcaga ggatcatcca ggtcatactg
    12541 atacgacgcg catggacaat gctgtggtga gaatccgccg ccataaaacg cgccagaatg
    12601 tgcggctgcc cgaagtaacc cagcccccag cccatcagcg agataatggc aacaaagttc
    12661 agacctttga gcatatcaac gttttcgatg ctcttttgtt tgatcacttc cagcgagtca
    12721 ccaaagccac cgacactgat aatgacgata accggtgtca ggatcagggc aaaaatcatc
    12781 aggctggcct gtacagtatc agtccagctc accgcgagga aaccgccaat aaaggtgtaa
    12841 aggatcgtcg ccgcagctcc cgcccacaga gccgtttcgt agctcatgcc aaaggtactt
    12901 tcaaacagac gcgcgcctgc cacaatgccc gaagcgcaat aaatggtgaa gaacagcaaa
    12961 ataaccagcg cggagataat gcgcaaaatg cggcttttat cttcaaagcg cccggtgaaa
    13021 taatccggca gggttaaggc gttattgttg tattcggtat gcacacgcaa ccgcccggcc
    13081 accagcttcc agttgatcca cgcgcctaat gtcaggccaa tggcgatcca gctttcggaa
    13141 atcccggaaa gaaaaaccgc gcccggcaac cccattaaca gccagccgct catatccgac
    13201 gcacccgccg ataatgccgt cacgaatggc ccaagactac gaccgcccag aatatagtcg
    13261 tcaaagtttt tcgttgatcg ccaggcgata aacccaatca atatcatgcc aaagatatag
    13321 acacaaaatg tcaccaacat cggtgtgcta atagccatct aaagtctcca aaaaattatt
    13381 atcggcaatg tcgaaacttg ccgttatatc tgccaccgga acggggtaac agagtttatg
    13441 ttttaccagg gcgaccgtat cctgccggaa gcgctggtta ttcacaatcg atttaacaca
    13501 ccatttacat taaattttag tgctcagcga cactattttt catcaggttg cactctctca
    13561 cattttttgc ggttgcacct ttcaaaaatg ttaactgccg cagagaaaaa gtctgagtta
    13621 tttttttaat ccctgtcata tcgatttctt ttattaacat ttcattcatt tttaagcttg
    13681 ctacgcaggt cacatttaac atggttgcac aaagttgcaa catcatggat atttcacgat
    13741 aacgttaagt tgcacctttc agaacaacag gagtaatggc atgggaacca ccaccatggg
    13801 ggttaagctg gacgacgcga cgcgtgagcg tattaagtct gccgcgacac gtatcgatcg
    13861 cacaccacac tggttaatta agcaggcgat tttttcttat ctcgaacaac tggaaaacag
    13921 cgatactctg ccagagctac ctgcgctgct ttctggcgct gccaatgaga gcgatgaagc
    13981 accgactccg gcagaggaac cacaccagcc attcctcgac tttgccgagc aaattttgcc
    14041 ccagtcggtt tcccgcgccg cgatcaccgc ggcctatcgt cgcccggaaa ccgaagcggt
    14101 ttctatgctg ctggaacaag cccgcctgcc gcagccagtt gctgaacagg cgcacaaact
    14161 ggcgtatcaa ctggccgata aactgcgtaa tcaaaaaaat gccagtggtc gcgcaggtat
    14221 ggtccagggg ttattgcagg agttttcgct gtcatcgcag gaaggcgtgg cgctgatgtg
    14281 tctggcagaa gcgttgttgc gtattcccga caaagccacc cgcgacgcgt taattcgcga
    14341 caaaatcagc aacggtaact ggcagtcaca cattggtcgt agcccgtcac tgtttgttaa
    14401 tgccgccacc tgggggctgc tgtttactgg caaactggtt tccacccata acgaagccat
    14461 cctctcccgc tcgctgaacc gcattatcgg taaaagcggt gaaccgctga tccgcaaagg
    14521 tgtggatatg gcgatgcgcc tgatgggcga gcagttcgtc acgggtgaaa ccatcgcgga
    14581 agcgttagcc aatgcccgca agctggaaga gaaaggtttc cgttactctt acgatatgct
    14641 gggcgaagct gcactgactg ccgcagacgc acaggcgtat atggtttcct atcagcaggc
    14701 gattcacgcc atcggtaaag cgtctaacgg tcgtggcatc tatgaagggc cgggcatttc
    14761 aatcaaactg tcggcgctgc atccgcgtta tagccgcgcc cagtatgacc gggtaatgga
    14821 agaactttac ccgcgtctga aatcactcac cctgctggcg cgtcagtatg atattggtat
    14881 taacatcgat gccgaagagg ccgatcgtct ggagatctcc ctcgatctgc tggaaaaact
    14941 ctgtttcgag ccggaactgg caggctggaa cggcatcggt tttgttattc aggcttatca
    15001 aaaacgctgc ccgttggtga ttgattacct gattgatctc gccacccgta gccgtcgccg
    15061 tctgatgatt cgtctggtga aaggcgcgta ctgggatagt gaaattaagc gtgcgcagat
    15121 ggacggcctt gaaggttatc cggtttatac ccgcaaggtg tataccgacg tttcttatct
    15181 cgcctgtgcg aaaaaactgc tggcggtgcc gaatctaatc tacccgcagt tcgcgacgca
    15241 caacgcccat acgctggcag cgatttatca actggcaggt cagaactact atccgggtca
    15301 gtacgagttc cagtgcctgc atggtatggg cgagccactg tacgagcagg tcaccgggaa
    15361 agttgccgac ggtaaactta accgaccgtg tcgtatttat gccccggttg gcacacatga
    15421 aacgctgctg gcgtatctgg tgcgtcgcct gctggaaaac ggtgctaaca cctcgtttgt
    15481 taaccgtatt gccgacacct ctttgccact ggatgaactg gtcgccgatc cggtcactgc
    15541 tgtagaaaaa ctggcgcaac aggaagggca aactggatta ccgcatccga aaattcccct
    15601 gccgcgcgat ctttacggtc acgggcgcga caactcggca gggctggatc tcgctaacga
    15661 acaccgtctg gcctcgctct cctctgccct gctcaatagt gcactgcaaa aatggcaggc
    15721 cttgccaatg ctggaacaac cggtagcggc aggtgagatg tcgcccgtta ttaaccctgc
    15781 ggaaccgaaa gatattgtgg gctatgtgcg tgaagccacg ccgcgtgagg tagaacaggc
    15841 gctggaaagt gcggttaata acgcgccaat ctggtttgcc actcctccgg ttgaacgcgc
    15901 ggcgattttg catcgcgctg ccgtgctaat ggaaagccag atgcagcaac tgattggtat
    15961 tctggtgcgt gaagccggaa aaaccttcag taacgccatt gccgaagtgc gcgaagcggt
    16021 cgattttctc cactactatg ccggacaggt gcgggatgat ttcgctaacg aaacccaccg
    16081 tccattaggg cctgtggtgt gtatcagtcc gtggaacttc ccgctggcta ttttcaccgg
    16141 gcagattgct gccgcactgg cggcaggtaa cagcgtgctg gcaaaaccgg cagaacaaac
    16201 gccgctgatt gccgcgcaag ggatcgccat tttgctggaa gcgggtgtac cgccaggcgt
    16261 ggtgcagttg ctgccaggtc agggggaaac cgtgggcgcg caactgacgg gtgatgatcg
    16321 cgtacgcggg gtgatgttta ccggttcaac cgaagtcgct acgttactgc agcgcaatat
    16381 cgccagccgc ctggacgctc agggtcgccc tattccgctc atcgctgaaa ccggcggcat
    16441 gaacgcgatg attgtcgatt cttcagcact gaccgaacag gtcgtcgtgg atgtactggc
    16501 ctcggcgttc gacagtgcgg gtcagcgttg ttcggcgctg cgcgtgctgt gcctgcaaga
    16561 tgagattgcc gaccacacgt tgaaaatgct gtgcggcgca atggccgaat gccggatggg
    16621 taatccgggt cgcctgacca ccgatatcgg tccggtgatt gatagcgaag cgaaagccaa
    16681 tattgagcgc catattcaga ccatgcgtag caaaggccgt ccggtgttcc aggcggtgcg
    16741 ggaaaacagc gaagatgccc gtgaatggca aagcggcacc tttgtcgccc cgacgctgat
    16801 cgaactggat gactttgccg aattgcaaaa agaggtcttt ggtccggtgc tgcatgtggt
    16861 gcgttacaac cgtaaccagc taccagagct gatcgagcag attaacgctt ccggctatgg
    16921 tctgacgctt ggtgtccata cgcgtattga tgaaaccatc gcccaggtca ctggctcggc
    16981 ccatgttggt aacctgtatg ttaaccgtaa tatggtgggc gcagtggtgg gtgtgcagcc
    17041 attcggcggc gaagggttgt ccggtaccgg gccgaaagca ggcggtccgc tctatctcta
    17101 ccgtctgctg gcgaatcgcc cggaaagtgc gctggcagtg acgctcgcgc gtcaggatgc
    17161 agagtatccg gtcgatgcac agttgaaagc cgcattgact cagccgctaa atgcactgcg
    17221 ggaatgggca gcaaatcgtc cagaattgca ggcgttatgt acgcaatatg gcgagctggc
    17281 gcaggcagga acacaacgat tgctgccggg gccgacgggt gaacgcaaca cctggacgct
    17341 gctgccgcgt gagcgcgtgt tgtgtattgc cgatgatgag caggatgcgc tgactcagct
    17401 cgccgccgtg ctggcggtgg gcagccaggt actgtggccg gatgacgcgc tgcatcgtca
    17461 gctagtaaag gcattgccat cggcagtcag cgaacgtatt caactggcga aagcggaaaa
    17521 tataaccgct caaccctttg atgcggtgat cttccacggt gattcggatc agcttcgcgc
    17581 attgtgtgaa gccgttgctg cgcgggatgg cgcaattgtt tcggtgcagg gttttgcccg
    17641 tggcgaaagc aatatccttc tggaacggct gtatatcgaa cgttcgctga gtgtgaatac
    17701 cgctgccgct ggcggtaacg ccagtttaat gacgatcggt taaacaggta gccggaggat
    17761 gttacaacct cctccggcat ctttaacgtg gtcgaatccc ctcaataata atccgctgca
    17821 cgttttcaac cgtttgattg aaaaatacct catcgcgcaa cgtcgcgcct gtcaccgcct
    17881 ccacctgagg ggcaaaatcg gcgtaatgtt gagtggaagc ccaaatcata aaaatcaaat
    17941 gctgcggatc aatcggcgcg agtttgccgc ttttgaccca accggcaatc agcgccgatt
    18001 tctcatcaat taatgccttc aaatcgcccg tcagttcatc cattaacagc ggcgcgcctg
    18061 ccagcatctc catacagaac aggcgcgaag cctgcggata atcgcgggag acttccagct
    18121 tcagacggat gtactctttg atcgccgcca gcggggcgaa atcttctcga aacgctttta
    18181 acggtgccag ccagatatcg agaatctgcc gcagcacggc aatatacagc gcctcttttg
    18241 acggaaagta atacagcaga ttggtttttg aaacacccgc caactctgcg atctgctcca
    18301 gccttgtgcc gtgaaaaccg aattgtgaaa aagtgtccag tgctgcgcta agaatcgctt
    18361 ttttcttcgc gcttactgcg cgcgaacgtt taccctttgt tttcactgcg ccttgcgtca
    18421 tgcgctctcc cctcttgtag attggcaaca ggatagcaaa acccccgccc ggcctcgacc
    18481 ttctgcactc tcatcgcgca cagatgcatg ttttatgtgc aactgttttg accgtttagt
    18541 ccacttttta ccagatattt tagataacgc attgttaaca tattaacaac attaactttt
    18601 taaaaactgg catccgcttt gcaaacaagc caatacgcgg ctgaaaagga tatgcaggat
    18661 gcagcgcccc gtctgacttt cactttacga gacgaagaga ggttgatgat gaaaattggc
    18721 gtattcgtac ctattggcaa caacggctgg ctcatttcga cccacgcgcc gcagtacatg
    18781 ccgacctttg aactgaataa agccattgtg caaaaagcgg agcactacca tttcgatttc
    18841 gccctgtcga tgatcaaact gcgtggcttt ggcggcaaaa ctgagttctg ggatcacaac
    18901 cttgagtcgt tcaccttgat ggcggggctg gcagccgtca cctcacgcat tcagatttac
    18961 gcgacagctg ccaccttaac gttacctccg gcaatcgtcg cccgtatggc cgcaaccatt
    19021 gactccatct ctggcgggcg ttttggcgtc aacctcgtga ctggctggca aaagcccgag
    19081 tatgagcaga tgggtatctg gcctggcgat gactatttct cccgtcgtta cgactatctc
    19141 accgaatatg ttcaggtgct gcgcgacctg tggggctcgg ggaaaagcga ttttaaaggc
    19201 gattttttca ccatgaatga ttgtcgcgtc agtccgcaac cgagtgtacc catgaaagtg
    19261 atctgcgccg ggcaaagcga cgctggcatg gcgttctccg cccagtatgc cgatttcaac
    19321 ttctgtttcg gcaaaggcgt aaatacaccc acggctttcg ccccgaccgc tgcgcggatg
    19381 aaacaggccg cagagcaaac cgggcgcgac gttggctctt atgtattgtt tatggtgatt
    19441 gccgatgaaa ccgacgatgc cgctcgcgcc aaatgggaac actacaaagc gggcgcggat
    19501 gaagaggcgt taagctggct aaccgaacaa agtcagaaag atacccgctc cggtactgat
    19561 accaacgtcc gtcagatggc cgatcccact tcggcggtaa acatcaatat ggggacgtta
    19621 gtcggttctt acgccagtgt cgcgcgcatg ttagatgaag tcgcaagcgt gcctggtgcc
    19681 gaaggcgtgc tgttgacctt cgatgatttt ctgtcgggaa tcgaaacctt cggcgagcgc
    19741 attcaaccac tgatgcagtg ccgcgcccat ctacctgcgc tgactcagga ggtggcatga
    19801 tgacaacctt aaccgctcga ccggaagcca ttaccttcga tccgcagcaa agtgcgctga
    19861 tcgtggtgga tatgcaaaac gcttatgcca cgccaggcgg ctacttagat ctcgccgggt
    19921 ttgatgtctc aaccactcgc ccggtcattg ccaacattca aaccgccgtg accgcagcgc
    19981 gagcggcagg gatgctgatc atctggtttc aaaatggctg ggatgaacag tatgtcgaag
    20041 ctggcggacc cggctcaccg aattttcata aatcgaacgc cctgaaaacc atgcgtaagc
    20101 agccgcagct gcaggggaaa ttgctggcga aaggctcctg ggattatcaa ctggtggatg
    20161 aactggtgcc gcagcctggc gatattgtgc tgccgaagcc gcgctacagc ggtttcttca
    20221 atacaccgct ggacagcatt ttgcgcagcc gtggaatacg ccatctggtt ttcaccggta
    20281 tcgctaccaa cgtctgcgtc gaatcgacgc tacgcgatgg cttttttctg gagtatttcg
    20341 gcgtggtgct ggaagacgca acacaccagg cggggccgga atttgcacag aaagccgcgt
    20401 tgttcaatat cgaaaccttt tttggctggg tcagcgacgt cgaaacgttc tgcgacgcgc
    20461 tttctcccac atcctttgct cgtatcgctt aaggagttta acgatgccaa aatccgtaat
    20521 tattcccgct ggcagcagcg caccgctggc ccccttcgtt cccggcacgc tggctgatgg
    20581 cgtggtgtat gtctccggta cgctggcttt tgatcaacat aataacgtgc tgtttgccga
    20641 tgacccaaag gcgcaaaccc gccacgttct ggaaattatc cgcaaggtga tcgagacggc
    20701 gggtggcacg atggcggatg tgaccttcaa cagcatcttt attaccgact ggaaaaatta
    20761 cgccgcgatt aacgaaatct acgccgagtt ttttccgggt gataaaccgg cgcgattctg
    20821 cattcagtgc ggactggtaa aacctgacgc gctggtggaa atcgccacaa ttgcgcatat
    20881 cgccaagtga ggccgcgatg aaactttcac tctcacctcc cccttatgct gatgcgcccg
    20941 tagtggtgtt gatttcgggt cttggcggta gcggcagtta ctggttaccg caactggcgg
    21001 tgctggagca ggagtatcag gtagtctgtt acgaccagcg cggcaccggc aataatcccg
    21061 acacgctggc agaagattac agtatcgccc agatggcagc ggaactgcat caggcgctgg
    21121 tagccgcagg gattgagcat tacgcggtgg tcggccatgc gctcggtgcg ctggtgggaa
    21181 tgcagctggc gctggattat cccgcgtcgg taactgtgct gatcagcgtt aacggctggc
    21241 tacgaataaa cgcccatacg cgccgctgtt ttcaggttcg cgaacgatta ctgtatagcg
    21301 gcggcgcgca ggcatgggtg gaagcgcagc cgttgttcct ctatcccgcc gactggatgg
    21361 cggcccgcgc acctcgcctt gaggcagaag acgcgctggc actggcgcat tttcagggca
    21421 aaaataattt actgcgtcga cttaacgccc tcaaacgcgc tgactttagt caccatgcgg
    21481 atcgcatccg ctgcccggtg caaatcatct gcgccagtga tgatctgctg gtgccatcag
    21541 catgttccag tgagcttcat gccgccctgc ccgatagcca gaaaatggtg atgcgctatg
    21601 gcggacacgc ctgcaacgtg accgaccccg aaacgtttaa tgctctttta ctcaacgggc
    21661 ttgccagcct gttacatcac cgcgaagccg ccctgtaagg aattgctatg aacgaagccg
    21721 ttagcccagg tgcgcttagc accctgttca ccgatgcccg cactcacaac ggctggcggg
    21781 agacacccgt cagcgatgag acgttacggg agctttatgc cctgatgaaa tgggggccga
    21841 catcagctaa ctgttctccg gcacggatcg tgtttatccg cactgtagaa ggaaaagaac
    21901 gtctgcgccc ggcgctttcc agcggcaatc tacaaaaaac cctgaccgcg cccgtcaccg
    21961 ctatcgttgc ctgggacagt gaattttatg aacggttacc actactgttt ccccacggtg
    22021 atgcccgcag ttggtttacc tccagcccac aacttgccga agaaacagcg tttcgcaaca
    22081 gctccatgca ggcggcctat ctgatcgtcg cttgccgtgc gctgggactg gataccggcc
    22141 cgatgtcggg ttttgaccgt caatacgttg acgacgcttt ttttgcaggc agcacgctga
    22201 agagcaatct gctgattaat atcggctatg gcgatagcag caaactttat gcgcgcctgc
    22261 cacgtctgtc ctttgaagaa gcctgcgggc tgttgtaagg agcaaactat gaacatagtt
    22321 gatcaacaaa cctttcgcga tgcgatgtcc tgtatgggcg cggcggtcaa tatcatcacc
    22381 acggacggtc cagccgggcg cgccgggttc accgccagcg ccgtctgcag cgtgaccgat
    22441 acgccgccca cattactggt gtgcctgaat cgtggggcgt ccgtctggcc ggtattcaat
    22501 gaaaatcgaa cgctgtgtgt aaatacactg agcgccgggc aggagccgct ttcaaatctt
    22561 tttggcggca aaacgcccat ggaacaccgc tttgccgccg cccgctggca gacgggtgtg
    22621 accggatgcc cgcaactgga agaggctctg gtttcgtttg actgccgtat cagccaggtg
    22681 gtgagcgtcg gcacccacga cattctgttt tgcgccatcg aagcgattca tcgtcacgcc
    22741 acaccctacg ggctggtgtg gttcgatcgc agttatcacg cgctgatgcg ccctgcttgt
    22801 taacctccta aaggagacag ctgatggcaa tgttcggttt tcctcactgg cagttaaaat
    22861 cgacctctac agagagcggc gtggtcgcgc cgtatgaacg attgccgttt gctcagacgg
    22921 cagtaatggg cgttcaacac gcggtggcga tgtttggcgc gacggtgtta atgccgattc
    22981 tgatggggct ggatcccaat ctttccattt taatgtcggg gatcggcacg ttactgtttt
    23041 tctttattac cggcgggcgc gtccccagtt atctcggttc cagcgcggct tttgtcggcg
    23101 tggtgattgc cgcaacgggc tttaacggtc agggcattaa cccgaatatc agcattgcgc
    23161 tgggcgggat tatcgcctgt gggctggttt ataccgtcat tggcctggtg gtgatgaaaa
    23221 ttggtacacg ctggattgaa cgactaatgc cgccggtggt gacgggtgcg gtggtgatgg
    23281 cgattggcct gaacttagcg cctatcgcag tgaaaagtgt atcggcttcg gcctttgata
    23341 gctggatggc ggtaatgacg gtgctgtgta ttgggctggt ggcggtgttt acccgtggca
    23401 tgatccagcg gttattgatt ctcgtaggat taattgtcgc ctgtctgctg tatggcgtga
    23461 tgaccaacgt tttagggctg ggaaaagccg tcgattttac tctcgtcagc catgctgcct
    23521 ggttcggtct gccacatttt tccactcctg cttttaatgg tcaggcaatg atgctgattg
    23581 cgcccgtggc ggtgattctg gtggcggaaa acttaggtca tctcaaggca gtcgccggga
    23641 tgaccggacg caatatggac ccgtatatgg ggcgggcatt cgtaggcgat ggtctggcaa
    23701 cgatgctttc cggctctgtc ggcggtagcg gcgtgaccac ctatgctgaa aatatcggtg
    23761 tgatggccgt gaccaaagtc tactcgacgc tggtgtttgt tgctgctgcc gtcatcgcca
    23821 tgctgttggg cttttcgccg aaatttggcg cgctgatcca taccattcct gcaccggtga
    23881 ttggtggtgc ttcaattgtt gtgttcgggc tgattgccgt cgcaggcgca aggatctggg
    23941 tacaaaaccg tgtcgattta agccagaacg gtaatttgat tatggtcgca gtaaccttag
    24001 tgctgggcgc gggtgatttt gctctcacgc tgggcggttt tacgttggga gggatcggta
    24061 cagcaacctt tggcgcgatt ttactcaatg cgttgttaag cagaaggttg gttgacgttc
    24121 caccgccaga agttgttcat caggagccat aaacagaaaa ccgccggggt aaccggcggt
    24181 gatgactttt tgacgagctt cagacaaaaa tgcattcgtc actgattagt ccggctagtt
    24241 gtcactttta ccgtgactgc tcttgccacc ttttttacct gcttcagatg cgcgctgcgg
    24301 gtcattcttg aaattacccc cgctgtgctg tccacctttt ttacctgctt ctgatgctct
    24361 ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta ctgacctccg
    24421 tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct gccatatttt
    24481 cgctacgtgg gactaagctt agtgaactga cgcgggcaga cccgcgtttg gtaatatcct
    24541 gcaacagctt cttagcgaga atagatatca tctcaaataa ttatccataa gccgctctta
    24601 tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt acgtgcttgt
    24661 gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa ggacgtctta
    24721 tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga gtggttagaa
    24781 atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac gatggcacgc
    24841 gcagtcgctg agggtgcaag caaagtcgat ggcgcagaag ttgtcgttaa gcgtgtaccg
    24901 gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac tgcaccggtt
    24961 gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc tacccgcttt
    25021 ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct gtgggcttcc
    25081 ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg cggcggtcag
    25141 gaacaaacta ttacttcaac ctggacgacc cttgcgcatc acggcatggt aattgtcccc
    25201 attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg tacgccgtac
    25261 ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga agaactgtct
    25321 attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa cggctaatct
    25381 tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc ggtgaatggg
    25441 catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc gccgggtacg
    25501 acgaaaaaat ggcggaaaag atttgggaag aaggtagcga tgaagtgtta gtcaaagcct
    25561 ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa cgtaaaaacg
    25621 tttaaccagc gactccccca cttctcgcgg gggagttttc tgttatttca ccgcctcatt
    25681 caacacgcta tcaaacttat ccattgggca gaaaccgttg gcatctatcg ggcaaccgct
    25741 taattccagc gtcacacgct gagcaggtgc ctgcagggtt aacgcatcgg cattacgtaa
    25801 ctgttccgca ctctgataca catattcaat tttcatcaaa tcgcgattgg ctttgctgtc
    25861 atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct ggtcatgcaa
    25921 ctgatacggt ttgaaatcca gcgccgttaa cagagaggca atgttggagt cgtgcccaac
    25981 caacactgta attttcggtg cgctggtgcg atcggtgacc agagctttgt cgatataact
    26041 gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacaggc tgtcctggta
    26101 gccgttttta agcttcgaca acaccttcca ctgctggtca gatttgattt ctccccaggc
    26161 cacctgatcc atcggaaaac cttcgtaata ttgcaaagta aacgcatcta ccagcgagtt
    26221 gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc taaaggtatt
    26281 tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat ctttatagtt
    26341 aacgattttt tccagtagtt ggtagctgtc ggtaagctgg agtttgctga gctctttctc
    26401 cattgccgcc accgcctgtt cactgaatgc agcggaatca tcggtgatca ccgggttaaa
    26461 ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac accccgggaa
    26521 tgcgccggta ataaagaact gtgcggtcgc aacggtacgt tgcagactat tggcataggc
    26581 ataaacggtg tccggcggcg ggcattcccc cgatttcacc atcccctgct gtgccagcca
    26641 ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg tgagttgccc
    26701 accggggacg tcccattcgg gccatttatt cggcgtagac tgctccagca cactgccatt
    26761 gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct gtagctgata
    26821 gccttccggt acggtttgtg cctgagcgtt tgaagcgagt aaaactatcc ctgccacagc
    26881 tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc actaaagagt
    26941 gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt tgaacgcctg
    27001 tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc ctgattagcc
    27061 cctgcctcgc gtacgccgct gataatttca cctgtggcta aacagaatat ctgcggcgtt
    27121 accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca atggttttta
    27181 gcatatgcct ttgccattaa attgcataac gatttaaata gtcgagttgc gcattcattt
    27241 ccttattttt taacttataa gcctgagcaa aaagttcagg agggaggtaa acatcataat
    27301 gattgccagc gt
//
LOCUS       DAPGWD010000052        27081 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPGWD010000052 DAPGWD010000000
VERSION     DAPGWD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.052"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(248..1840)
                     /gene="lsrK"
                     /locus_tag="RHX88_002119"
     CDS             complement(248..1840)
                     /gene="lsrK"
                     /locus_tag="RHX88_002119"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDV2230808.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1919..2872)
                     /gene="lsrR"
                     /locus_tag="RHX88_002120"
     CDS             complement(1919..2872)
                     /gene="lsrR"
                     /locus_tag="RHX88_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDV2230809.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3121..4656
                     /gene="lsrA"
                     /locus_tag="RHX88_002121"
     CDS             3121..4656
                     /gene="lsrA"
                     /locus_tag="RHX88_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDV2230810.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     VSC"
     gene            4650..5678
                     /gene="lsrC"
                     /locus_tag="RHX88_002122"
     CDS             4650..5678
                     /gene="lsrC"
                     /locus_tag="RHX88_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDV2230811.1"
                     /translation="MLKLIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5678..6670
                     /gene="lsrD"
                     /locus_tag="RHX88_002123"
     CDS             5678..6670
                     /gene="lsrD"
                     /locus_tag="RHX88_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDV2230812.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6682..7704
                     /gene="lsrB"
                     /locus_tag="RHX88_002124"
     CDS             6682..7704
                     /gene="lsrB"
                     /locus_tag="RHX88_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDV2230813.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7731..8606
                     /gene="lsrF"
                     /locus_tag="RHX88_002125"
     CDS             7731..8606
                     /gene="lsrF"
                     /locus_tag="RHX88_002125"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDV2230814.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8630..8920
                     /gene="lsrG"
                     /locus_tag="RHX88_002126"
     CDS             8630..8920
                     /gene="lsrG"
                     /locus_tag="RHX88_002126"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDV2230815.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8977..9735
                     /gene="tam"
                     /locus_tag="RHX88_002127"
     CDS             8977..9735
                     /gene="tam"
                     /locus_tag="RHX88_002127"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDV2230816.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLIESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9739..10653)
                     /locus_tag="RHX88_002128"
     CDS             complement(9739..10653)
                     /locus_tag="RHX88_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDV2230817.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10860..12311)
                     /gene="uxaB"
                     /locus_tag="RHX88_002129"
     CDS             complement(10860..12311)
                     /gene="uxaB"
                     /locus_tag="RHX88_002129"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDV2230818.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12538..13956)
                     /locus_tag="RHX88_002130"
     CDS             complement(12538..13956)
                     /locus_tag="RHX88_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV2230819.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLSF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14095..14454)
                     /locus_tag="RHX88_002131"
     CDS             complement(14095..14454)
                     /locus_tag="RHX88_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDV2230820.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14454..15380)
                     /gene="glsB"
                     /locus_tag="RHX88_002132"
     CDS             complement(14454..15380)
                     /gene="glsB"
                     /locus_tag="RHX88_002132"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDV2230821.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15444..16832)
                     /gene="sad"
                     /locus_tag="RHX88_002133"
     CDS             complement(15444..16832)
                     /gene="sad"
                     /locus_tag="RHX88_002133"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDV2230822.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16933..17814
                     /locus_tag="RHX88_002134"
     CDS             16933..17814
                     /locus_tag="RHX88_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2230823.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17892..19007
                     /gene="yneK"
                     /locus_tag="RHX88_002135"
     CDS             17892..19007
                     /gene="yneK"
                     /locus_tag="RHX88_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDV2230824.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPARNGRHKENI
                     EFQVVINLIPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMINPGVPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDGELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            19157..20347
                     /gene="ydeA"
                     /locus_tag="RHX88_002136"
     CDS             19157..20347
                     /gene="ydeA"
                     /locus_tag="RHX88_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDV2230825.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20372..21037)
                     /gene="marC"
                     /locus_tag="RHX88_002137"
     CDS             complement(20372..21037)
                     /gene="marC"
                     /locus_tag="RHX88_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDV2230826.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21249..21683
                     /gene="marR"
                     /locus_tag="RHX88_002138"
     CDS             21249..21683
                     /gene="marR"
                     /locus_tag="RHX88_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDV2230827.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21703..22086
                     /gene="marA"
                     /locus_tag="RHX88_002139"
     CDS             21703..22086
                     /gene="marA"
                     /locus_tag="RHX88_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDV2230828.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22118..22336
                     /gene="marB"
                     /locus_tag="RHX88_002140"
     CDS             22118..22336
                     /gene="marB"
                     /locus_tag="RHX88_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDV2230829.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22393..23832)
                     /locus_tag="RHX88_002141"
     CDS             complement(22393..23832)
                     /locus_tag="RHX88_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV2230830.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYAQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23857..25530)
                     /locus_tag="RHX88_002142"
     CDS             complement(23857..25530)
                     /locus_tag="RHX88_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDV2230831.1"
                     /translation="MNIKALNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25586..25897)
                     /locus_tag="RHX88_002143"
     CDS             complement(25586..25897)
                     /locus_tag="RHX88_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDV2230832.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25925..>27081)
                     /locus_tag="RHX88_002144"
     CDS             complement(25925..>27081)
                     /locus_tag="RHX88_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDV2230833.1"
                     /translation="SPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFL
                     VARNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVAL
                     ITSEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLALISPAG
                     LNDLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLE
                     NLSWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKM
                     SFIPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPW
                     TTPGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT         7001 a         6772 c         6425 g         6883 t
ORIGIN      
        1 gctaaaaatc gaagtttatt aaaccccttt tgcatccatt agtgtcttta ggaatattcg
       61 ctataaaata agggttgtgc ttaatgcttt aagaaaaata gcaattttcc cttgaatata
      121 gtactggtga tggaacgatg aatctgcaag ctggctttta acaagccagc tctaaaagaa
      181 gggaaataag aataactata ctcaaaaact aacagccacg gtcatcatga tgtggctgtc
      241 aatgaaacta taacccaggc gctttccata acgacgtcgt cagtccatga tcaaccagcc
      301 ccagctgatc ctgataaact gcctgccact tatcgcgtga atcctgataa agttcatgct
      361 tttccgggtc tggtgtgtgc gtccgttccc agcgaaccag gcgttctccg gtttctgcca
      421 ttgatgaaaa aattccggca ccgacgccag ctgcaatggc acatcctaat gcagtggctt
      481 ctttgaccac cggaatattg acgggtaatc ccgagacatc agcgagaatt tgactccata
      541 atttcccttt tgaacctccg cctgcaaaga ctaacgatga aggatgaata ttcgagaaat
      601 cagcaatttg ctgcaagtta cacgctgata caatcgccgc attttcttcc agcgcacgga
      661 acaatgtcgc tttgttacat ttatccgggt caatggacaa gttaataaag gaaggcgcag
      721 cgtgatacca ggttttaaag cgcattctgt cggagaagat cggcattacg ccccacgacc
      781 caggcggcac ccgactggcc atctcttcca gcagcgtata ggtgtcgatg cctaaacgtt
      841 cggcaatcag tttttcttcg gcacagaaag catcgcggaa ccagcgcatg gtgagtccgg
      901 taaaaaagct tatagattca gcttgtacca tgccaggaat aacatgagga ttaacgcgca
      961 cgttcatttc tgggtctgtc accggcgcgg ctaaatttac aacttgctgc cagaatgtgc
     1021 cgccaagaac cgcggtttgt gccggacgca caacgcctaa cccaaggcaa ccaagctgca
     1081 cgtcgcctcc tccaacgacc accggagtgc ccgccttcag accgcagagt tccgccgctt
     1141 gtgaacttac cacgcccagc aatgtgccgg tttctttgac aggagaaaga atatcggcac
     1201 gcaggccagc catatccagc aatgcaggtt tccagtcacg ggtggttaga tcaagaagtc
     1261 ccgtggtgcc agcgttagag ggatccaccg ccagttcgcc gctgagcata taggccagcc
     1321 agtcgctgat catggtgatg gttgatgcct gacggtaaat atcggaacga tggtgcgcca
     1381 gccaaagtaa tctggggatg gcacttaaag ccagtgtttg tccggtcgcg cgataaactt
     1441 cgttttcaaa ggtattgttg tgcagttctt taagttcgct aacttcgcgt gccgctctgg
     1501 catccacatt ggcgcaggcc cagatcgggg ctccttcatt attatataaa acaatgcctt
     1561 cacgcatcga acatgccgaa acggcagcga tatactccgg ggctatgccg gcgttgtgca
     1621 gcgcctggcg catacactca cacgccagtt gccagttttt gttgagatca aattccatag
     1681 aaccaggaac gtccggtact gccaggtgcc gccactccgc ctgtcccact gctatttgat
     1741 tgccttccag gtcgaatatc acagcccgaa tacttccggt acctgcatcc agcgccatca
     1801 ggtaatactt tgattctgaa ggggtaaaga gtcgagccat tagattatcc tcggcttatg
     1861 cgttctgtct gttcctctat acgttctcca tcattcccgg taataaggtc atgcaaattt
     1921 aactacgtaa aatcgccgct gctgtgtcct gatcggtaac cagtgcgttg atataaccgc
     1981 ctttcattgc agcggcaatt gcttcggctt tattttctcc ccctgccacg ccaacccgga
     2041 cgggtatggt cttcagcgag cttaaaggta agccaatcag ttcgttatgt attttaatat
     2101 ccgtgacaac gtcacctttt gcatcaaaaa agtagcctaa aatgtcgcca accgcccctt
     2161 ttcgaccaat cattaactgt tcgccctggc tgatataacc ggagcgaatg atcgtcgcgt
     2221 cgtcctgttg actgacggcc ccaatgccga caatcgccac atcggctgct tgcgcggcta
     2281 acagaacatc tttgacgcaa ttttcatttt ttagcgtacg ggcaatgtca gcggaggatg
     2341 cccgcaacgg agccggaata atattcacac tgcacgccgc gttaagctgc ccgatacccg
     2401 tcatataaga accgacgcca ccggagagcg tgaccaggcg aatttgctgt gacgaaataa
     2461 aaccacttaa gcgttgcagc gtattcatgg ttgcctcgcc aaaaccaatc gccagcatct
     2521 gttgtggttg aagtaaactc atcaacatat gcgccgcgcc tatccccagt cgcccgccga
     2581 catcagcatc cgcaagccca gggatcaccc ggacatgttg cagcgaaaac tgacgacgta
     2641 attgagtttc atattccaga cagccttcaa agcgagaatt aatctgtacg cgaataatgc
     2701 cggactgatg ccctttctcc agcaagcgcg acactttcaa acgggtcagg ccgagacgat
     2761 cgctgatctc gctctgggtc agcccgtcgt gatagtaaaa ccacgcgatc cgcgcgacct
     2821 gttcttcttc acacattccc tgttctgaaa ttgccgaatc gttgattgtc ataattcatt
     2881 cttcactttg aacatattta aatctttaat gcaattgttc agttcttgcg catttatatc
     2941 tgtgatggca gccacagttt gactctacga gcatgaacaa acgcaaccgt gaaaatcaaa
     3001 atagcataaa ttgtgatcta ttcgtcggaa atatgtgcaa tgtccaccta aggttatgaa
     3061 caaattaaaa gcagaaatac atttgttcaa aactcacctg caaaactgaa cgggggaaat
     3121 atgcaaacga gtgatacccg cgcgttaccg ctactttgcg cccgctcggt ttataaacag
     3181 tattcagggg tcaatgtcct gaaaggcatc gattttacgt tgcatcaggg ggaggtccac
     3241 gccctgctcg gcggcaatgg tgccggtaaa tcgacgttaa tgaagattat tgccggtatt
     3301 acccctgctg atagcggtac gctggagatt gggggcaaca actacgccag attaacgcca
     3361 gttcatgctc atcagctggg catttatctc gttccccagg aaccgctgct tttcccaagc
     3421 ctgtcgataa aagaaaacat cctgtttggg atggcaaaaa aacagctctc catgcaaaaa
     3481 atgaagaact tgctggcggc gctgggctgc cagtttgatc tgcatagtct ggcaggatcg
     3541 ctggatgtcg ccgatcgcca aatggtggaa atcctccgcg ggctaatgcg cgactcgcgg
     3601 attctgatcc tcgatgaacc taccgcctcg cttacccctg cggaaaccga acgcttgttt
     3661 actcgcttgc aagagctgct tgctactggc gtgggtattg tttttatctc gcataagctg
     3721 ccggaaattc gccagattgc cgatcgaatt agcgtgatgc gcgacggaac catcgcctta
     3781 agcggcaaaa ccagcgaact gtctaccgac gacattattc aggccatcac gccagtggta
     3841 cgggaaaaat cgctctctgc cagccaaaaa ttatggctgg agttacctgg taaccgccca
     3901 caacatgctg ccggaacgcc ggtgctgaca ctggaaaatc tgaccggcga aggtttcagg
     3961 aatgtcagca tgacgctcaa tgccggagaa attctgggcc tggctgggct ggtgggggcc
     4021 ggacgcacag aactggccga gacgctctat ggtctgcgta ctttgcgtgg cggacgcatt
     4081 atgctgaatg gtaaagagat caataaatta tccactggag aacgtttact gcgcggtctg
     4141 gtttatctgc cggaagatcg ccagtcatcc ggactgaatc tcgatgcttc gcttgcctgg
     4201 aacgtctgcg cccttactca taaccttcgt ggattctggg cgaaaaccgc gaaagataat
     4261 gccaccctgg aacgttatcg tcgggcgctg aatattaaat ttaaccaacc ggaacaagct
     4321 gcacggacat tatccggtgg caaccagcaa aaaatcctca ttgccaaatg cctggaagct
     4381 tcgccgcaag cattgattgt cgatgagccg acgcgcggcg tggatgtctc ggcccgtaat
     4441 gatatctacc agctgttgcg cagcatcgcc gcacaaaatg tggcagtgct gcttatctcc
     4501 tccgatctgg aagagatcga actgatggct gatcgcgtgt atgtgatgca tcagggcgaa
     4561 attgcccact ctgcactgac cgggcgcgat attaatgtcg agactattat gcgcgttgcc
     4621 ttcggcgata gtcagcgtca ggaggtgtca tgctgaagct tattcagaac aaccgtgaaa
     4681 tcacggcact gctggcggtg gtgctgctgt ttgcattacc cggttttctc gaccgccagt
     4741 atttaagtgt gcaaacgctg accatggttt atagcagcgc gcaaatcctg atcctgctgg
     4801 caatgggcgc gacgctggta atgcttacgc gcaatattga tgtttcagtg ggttcgatta
     4861 ccggaatgtg cgcagtgctg ttggggatgt tactgaacgc ggggtattca ctacctgttg
     4921 cttgtgtcgc gactttactg cttggtttgc tcgcgggatt tttcaacggt gtcctggtcg
     4981 cgtggctaaa gatccctgcc attgttgcca cccttggcac gttagggttg tacagaggca
     5041 tcatgttgct gtggactggc ggtaaatgga ttgaagggtt acccgccgaa ctgaaacagc
     5101 tctccgcacc gctgctgttt ggcgtttccg cgattggttg gttgacgata attctggtgg
     5161 catttatggc ctggctgctg gcaaagacgg cgtttggacg cagtttttat gccacgggcg
     5221 ataatttaca gggcgctcgt caactgggtg ttcgtacgga agccattcgc attgtggcat
     5281 tttcgttgaa cggctgcatg gcggcactgg cgggaattgt gtttgcttcg cagattggtt
     5341 ttatccccaa ccagaccggt accgggctgg agatgaaagc aattgcagcc tgcgtgctgg
     5401 gcggcattag tttgctcggc ggttccggtg cgatcatcgg tgcggtactc ggcgcatggt
     5461 tcctgacgca gatcgatagc gtactggtgc tgttgcgcat tccggcatgg tggaacgatt
     5521 ttatcgcggg cctcgtgctg ctggcggtgc tggtgtttga tggacgcctg cgttgtgcgc
     5581 tggaacgtaa tctacggcgg caaaaatatg cccgctttat gacgccaccg ccatccgtta
     5641 aacccgcttc gtcaggtaaa aaacgggagg ccgcataatg cgtattcgct acggttggga
     5701 actggctctt gccgcactgc tcgttattga gattgtcgca tttggtgcaa ttaacccgcg
     5761 aatgttagat ctcaatatgt tgctgttcag caccagtgac tttatctgca ttggcattgt
     5821 cgccctaccg ctgacgatgg tgattgtcag tggcgggatc gatatttcgt ttggttcgac
     5881 catcggcctc tgcgccattg cattgggcgt actgtttcaa agtggtgtgc cgatgccgct
     5941 ggcgatactc ctgaccttac tgctcggcgc attgtgcggg ctgatcaacg ccggattaat
     6001 tatctatacc aaagttaacc cgctggtgat tacgcttggc acgctgtatc tgtttgccgg
     6061 aagcgctctg ctgctttccg gtatggccgg agcgacgggg tacgaaggta ttggtggatt
     6121 cccgatggcg tttacagatt tcgctaacct ggatgtgctg ggactccccg ttccgctgat
     6181 tatcttcctg atatgtctcc tcgttttctg gctctggctg cataaaaccc atgccggacg
     6241 taatgtgttt ttgattgggc aaagcccgcg cgtggcgctt tatagcgcga ttccagttaa
     6301 ccgcacctta tgtgcgctct atgccatgac ggggctggcg tctgcggtcg ccgctgtgct
     6361 gctggtatcg tattttggtt cagcacgttc cgatctcggt gcgtcgtttc tgatgcccgc
     6421 catcaccgcc gtggtgcttg gcggtgccaa tatttatggt ggttccggtt ccattatcgg
     6481 caccgccatt gcggttttat tagtgggata tttgcaacaa ggtttgcaaa tggcaggagt
     6541 gccaaatcag gtgtccagcg ccctttccgg tgcgctactt atcgtcgttg tcgtaggtcg
     6601 ttccgttagc ctgcatcgcc agcaaattaa agagtggctg gcgcgtcggg ccaataaccc
     6661 attgccataa aggatatctt catgacactt catcgcttta agaaaatcgc cttacttagc
     6721 gctcttggca ttgccgcaat ctctatgaat gtgcaggccg cagagcgtat tgcatttatt
     6781 cccaaactgg ttggcgtggg attttttacc agcggtggca acggcgcaca acaagcgggt
     6841 aaagagctga gcgttgatgt gacctacgac gggccgacag aacccagtgt ttctggtcag
     6901 gtacagttga ttaataactt cgtcaatcaa ggttataacg ccattatcgt ttctgcggtt
     6961 tcgcctgatg gcttgtgtcc ggcactgaaa cgcgccatgc aacgtggtgt gagagtgctg
     7021 acctgggact ctgatactaa accggagtgc cgctcttact acattaatca gggaacgccc
     7081 gcccagttgg gaggtatgtt ggtggatatg gcggcgcgtc aggtgaataa agacaaagcc
     7141 aaagtcgcgt ttttctactc aagccccacc gttacggacc aaaaccagtg ggtgaaagaa
     7201 gcgaaagcga aaatcgccaa agagcatcct ggctgggaaa ttgtcactac gcagtttggc
     7261 tataacgatg ccactaaatc attacaaacc gcagaaggaa tattaaaagc gtatagcgat
     7321 ctcgacgcca ttatcgcccc cgacgccaac gccctgcccg ctgccgcaca agccgcagaa
     7381 aacctgaaaa atgacaaagt tgcgattgtc ggattcagta cgccaaacgt gatgcgtcca
     7441 tatgtggaac gcggcacggt gaaagaattt ggcctgtggg atgtggttca gcaaggcaaa
     7501 atttcagtgt atgtcgcgga tgcattattg aaaaaaggat caatgaaaac gggcgacaag
     7561 ctggatatcc agggcgtagg tcaggttgaa gtctcgccaa acagcgttca gggctatgac
     7621 tacgaagcgg atggtaatgg catcgtactg ttaccggagc gcgtgatatt caacaaagag
     7681 aatatcggca aatacgattt ctgatgtgca ttacttaacc ggagtaagtt atggcagatt
     7741 tagacgatat taaagatggt aaagattttc gtaccgatca accgcaacaa aatatccctt
     7801 ttaccctgaa aggttgcggt gcgctggatt ggggaatgca gtcacgctta tcgcggatat
     7861 ttaatccgaa aacgggtaat accgtgatgc tggcctttga tcatggttat tttcaggggc
     7921 cgactaccgg acttgaacgc attgatataa atatcgcccc gctgtttgaa catgccgatg
     7981 tattaatgtg tacgcgcggc attttgcgca gcgtagttcc cccagcgacc aataagccgg
     8041 tggtactgcg ggcgtcaggt gcgaactcta ttctggcgga attaagtaat gaagccgtgg
     8101 cgttatcgat ggatgatgcc gtgcgcctga acagttgcgc ggtggcggcg caggtttata
     8161 tcggcagcga atatgaacat cagtcgatca aaaatattat tcagctggtt gatgccggaa
     8221 tgaaagtggg aatgccgacc atggccgtga ctggcgtggg caaagatatg gtgcgcgatc
     8281 agcgttattt ctcgctcgcg actcgaatcg ccgctgaaat gggggcgcaa attatcaaaa
     8341 cctattatgt cgaaaaaggt tttgaacgga ttgttgccgg atgtccggta cccattgtta
     8401 ttgctggcgg taaaaaatta ccggagcgcg aggcgctgga aatgtgctgg caggctatcg
     8461 atcagggcgc ttctggtgtg gatatggggc gtaatatttt ccagtctgac catccggtgg
     8521 cgatgatgaa agccgtacag gcggtggttc accataacga aacggctgat cgggcatatg
     8581 aactctatct gagtgaaaaa cagtaactgc ggatttaagg agaagaatta tgcacgtcac
     8641 actggttgaa attaacgttc atgaagacaa gattgacgag tttatcgaag tttttcgcca
     8701 gaaccacctg ggctctgtac aggaagaagg caatttgcgc ttcgatgtct tacaggaccc
     8761 agaagtgaat tcgcgctttt atatttacga agcctataaa gatgaagacg cagtggcatt
     8821 ccataaaacc acgcctcact acaaaacctg tgtcgcgaaa ctggaatctt taatgaccgg
     8881 gccgcgtaaa aaacgtctgt tcaatgggtt gatgccgtga ggcgaattta tcatttttat
     8941 ctacaattgg ggtaacgcgc tgacgggagt aaaaatatgt ctgactggaa cccctcttta
     9001 tatctgcact ttgccgctga acgatcgcgt ccggcggtgg agctgcttgc cagagtgccg
     9061 ctggagaata tcaaatacgt tgccgatctt ggctgtggcc caggtaacag caccgccctg
     9121 ctgcatcaac gttggcctgc ggccaggata acaggcatcg actcgtctcc ggcgatgatt
     9181 gctgaagcgc gcagtgcttt gccagactgc cagttcgtgg aagcagacat ccgcaactgg
     9241 caaccggaac aggcactcga tctgattttt gctaatgcct cactgcaatg gctgcccgac
     9301 cactacgaat tgtttcctca tctggtttct ttacttaatc cgcagggcgt gctggcagta
     9361 cagatgccag ataactggct ggagccgacc catgtgctca tgcgcgaagt tgcctgggaa
     9421 caaaactacc cggatcgcgg gcgtgagccg ttggctggcg ttcatgctta ctacgatatt
     9481 ttgagcgaag ccggatgtga ggtcgatatc tggcgaacca cctactatca ccagatgccg
     9541 tcacaccagg cgattatcga ttgggtgact gccactggat tacgtccgtg gttacaggat
     9601 ctgatcgaga gcgaacagca gctttttctt aagcgctacc atcagatgct ggaagagcag
     9661 tatccactgc aagagaacgg acagatactg ctggcatttc cgcgtctttt tattgttgcc
     9721 cggcgtatgg agtaaatatc acgtcagctg gtaatgacga tcgggaagaa tttttgctgg
     9781 aatttcggct tcatcgttca tctgtaacag gtcaatttca atagcgttgc agatggcatc
     9841 cagcggtaaa tcattgtttt cagtaccgaa cggatcttcc agttcttccg ccagacaatc
     9901 cagcgaaata aaagtgtagg aaatcagcac agagataaaa ggcgtcatgt aatgcaggtc
     9961 cacgaccagc gcgaacggca gcatgataca aaacagataa acggtacgat gcaaaatcag
    10021 ggtgtaggca aagggaattg gcgtataggc aatgcgctcg catcctgcca ggaccgctga
    10081 aatatcatta agccgatcgt tgaggctaat aaacaggata tctgaaagct gcccattgcg
    10141 gcgctgaacc gccaaccact cccccattat taacaagata cggttagctg gagagttcga
    10201 agccagtaca ggctgaagat cttcagtctt gagataatga gccagcactt ccgcctgtgg
    10261 ctgtttgcgt aatgtcatgc gtaaacagtg ggcgaaggcg atttgcaacc gggcaaactc
    10321 ccttacactt gccgaatccg gcaatgtcgt ttttacctcg cgcagtaacg accgtgaggc
    10381 aatcatcaac tgcccccaaa gttttcgcgc ttcaacgtaa cgggcgtacc cggcattatt
    10441 acgaaaacca agaaaaatgg cgatggcgac accgagaatg ctgaacggtg cgagggtgaa
    10501 tttgatgccc agatgcgtgt accagggcag catgaaaata acagcgatag aaaagagaaa
    10561 attgagtagt aagcgcgatg atatcttgga taatactgag ccgtgccaga caaaaatacg
    10621 gcgcagccag tgttgttgtg gacgaacaat catggttatc ttcaggcgtg aaaaagtcgc
    10681 cctattaaac gtgattacga tcacattctc aagacgctac ttacaaatta cctacaagct
    10741 tacaacatta acaggcgctg catgtggcat ctgttaatga taaaggcaca taggatgttg
    10801 taactaatat agtgaaataa gaaacccggt cgaaaccggg ttcagaagta acggtgttat
    10861 tagcacaacg gacgtacagc ttcgcgcatc cctttttcaa gaatcgcatc caggtcatta
    10921 gcaacctgct cgaccagacc aggcacttgc gtcaggtcct gctcccagtg gtctttttct
    10981 gccaacacaa tcgctaccag ttcctgggta ccgataacac ggtcacgatg ctggctccag
    11041 agttgctgat aacgttccag ccagtgtgca tcgtcctgta ccggatatgt ttctccgtta
    11101 cgctcaccac gatagaaggc aatcaatgcc gctaatgcga aagtaaggcg cgccggaagt
    11161 gtgccttttg ccttctgccc tgccagcagc tgcggcagga tgcgagtgcg gaacttggtc
    11221 ataccgttga gcgcgataga cagcagctga tgcttaatgt acgggttacg gaaacgcccg
    11281 gtgactgcac tggcgaaaga ttccagttca tcacgaggca aatccagtac cggaataatt
    11341 tcttcgtaga tagctttttc aacgaatgcg caaatttcag catcgttcat cgcttcacct
    11401 acggtatcca gccctgcctg gaacgccacc ggcaccagcg cggtgtgcgc accgttgagg
    11461 attgccactt tgcgttcttt gtacggttta atatcgtcaa caatcagcac gttgagcgga
    11521 tatttgtcca gacgcagttc agtcgctaag gatttcggcc cctgaatcac aaacaggtaa
    11581 aagtgttcag cggtgtcgag gaaaccatcg tgataaccca gttcttcttc cagtttagcc
    11641 acttcatcgc gcggataacc ggtaacgata cggtccacca gcgtagaaca gaagctgtta
    11701 gcctgatcca accattgaat aaatgcttct ggcagtgccc actcttgagc atagcgcagc
    11761 accagttcac gcaacgcgtc gccattgtag tcgatcaact cacacgggat gatgatccaa
    11821 cctttatcca gcgcaccgtt gaaatggcta aaacgttcga acagcagacg ggtcagtttt
    11881 gcgggatagc ttaccgctgg cgcgtcatcg aatttatcgc ccgcatggta gctgataccc
    11941 gcttcggtgg tgttagagaa aacaaagcgc atttccggat tgtgcgccag tttcaggaat
    12001 tcatcgtatt cactgtagac gctgatttca cgattaacag agcgaatcag acgcgcgtcg
    12061 ctgaccgctt cccccttctc attcaggcca cggataatgg tggtgtacag accatcctgc
    12121 gtgctcagtg acggcgggaa tgaagtttca atcggacgaa caacgaccac gccagaattc
    12181 agatcggtgt gctcattcag gagatcgatt tgccagtcaa caaaggcgcg caggaagtta
    12241 ccttcaccaa actgaatgat acgttctgga tactgtgcac cgggaaaatc gcgacgattt
    12301 agtgttttca caatgggttc ccttctgatt agtcatacaa cctgtttgaa ttggtacgac
    12361 aggttagcaa actttaatac gccgaacccc tgttttgatc aactcctgat gattaatgag
    12421 cagttttatg agaaaagtgt ggcgcggatc atggtttaat cgaggaaata cgccttttcc
    12481 tggatcataa agtggtagaa cacattgcat tcaaatcgcg cggaatgaat aaagatgtca
    12541 gacaacttcc tcaccgtaac gcatagtgct ggtacggttg cgcccatctt tcttcgagcg
    12601 atacagacac gtatcggctt cgaccatcaa tttattaaaa tcatcggtca gcgttcggta
    12661 cgatgcgcga ccactaccga cgccaatact taccgtgaga taaagcgttt tttgttgcca
    12721 ggtgaatggt tgcagttcaa cgcctttacg gattttttcc gccattagca gaccatcgac
    12781 aggattcacc gacggcactg caacagcaaa ttcttcgccg cccatgcgcg ccaccagccc
    12841 cttatcaccg acaatcttct gaatatgccg ggcaaacacg cttaacactt tatcgccaca
    12901 ttcatgtcca tagttatcgt taatgctttt gaaatagtca atatcaagca gcatgaccgt
    12961 cagatgttgt gtctgtttca gcgatggact tttcagcgcc tcataaagac cggaccgtga
    13021 gtaaacctga gtcagaaaat caaagtcggc tcgcagcgca acttgcttca ttagcgaatt
    13081 gatcgctgcc acgctaaaag aaaccataat tgggcatatc gccatcgtgg caataccgag
    13141 acgtgcggag aacatttccg gaatggagaa cggcgaaccg accgagatat caatcaccga
    13201 atttgccacc agtacgattt ccaccgcacc ggtgacaaag gtcaacagac atgttacctg
    13261 cggcgtatag cgcactgcac accagattaa tgcaggcagc gggaacgaca gactccccgc
    13321 tccgccaatg actaccgagg caataaccga cacaataagc gcgatggcgg gcatcatctg
    13381 ttctgcttta aagcgcggca gcactccagg aatagccaac gtcagcatac aaggcacgat
    13441 caacacgccc gttgagaatt gctcactgaa ccagtccgca agcaaaggcc agaaatccag
    13501 actatcaata ctgaccgaac caatcgcccc gacaatagcg cataataatg cggcaatcag
    13561 acagtaatta aatagccgta aggcacttac tggctcatac ttatttttcc ccagacgctt
    13621 atcccgcacg accagtaagg cgacggtaac aataaacatc atattggaga aattgataac
    13681 cagtgaaacc agtccccatt cggtggttat ggcatcataa accagcatcg ccacataact
    13741 gatcgcatag taatgcagtc gattaagcca gacatagcgg gcaaacaccc ccgccattac
    13801 gccattcagc ggccagaaaa gagaaagttc gtgaaccagc cgtaactctg caccaataaa
    13861 atagaacagc gtggttagcg caaaaatagc gatgctatta cgtagcagat gaccttcctg
    13921 gaacaaacgg aaagttgaaa tggggtgtac atgcattaat tatctgtcca tacaattgcg
    13981 ggtggcaatt atctcaacgt aataagtaaa ttcacataaa ggatagcatg ttcgctaaca
    14041 gcggcggcaa ataaaggtac cctgggaatg ctattttatc gttttccgca attctcatgg
    14101 ctgattcatc tgtacaacca gccagtgata gattaccgcc acgatataac gttgttgttc
    14161 ttcgcttaac gaaacaccct gcggaatgtt atgccactta gccagacaac cgcgacagca
    14221 ggtcgcggtg gcgtgctgag cgataaacac cggatgcccg cgcatggggg tttgcttacc
    14281 gtcattagcc ggtaatgccg gagcaagacg tttagcgaca aaatccgccg catgttgttc
    14341 gatgactggc gcgcctttct ccaggcaata ctgacgctct ttcatgccca gacgaaagcg
    14401 agagcgaaat tttgaacggg ataaacgcgc gaagagtgga tcgagagact gcattaataa
    14461 accgaacgcc ctaactgttt cgtcaattgt tcaaggacgg cgatacccgc aagcgagtta
    14521 cctgcatcat ccagttccgg actccagact gcgatggcca tttcatgcgg aacaatcgcc
    14581 acaataccgc caccaacgcc agatttcgcc ggtagtccca cccgccaggc aaactccccc
    14641 gcgttctggt acataccact ggtcgccatc agcgcgttaa tttgccgcgc ctgcattggc
    14701 gtcaccacag gttcatcaat atgaatagct ttcccctgat tagccagaaa gacaaacgtc
    14761 cgggccagct ctacacagct cattttcaga gcgcagtaat gaaagtagtt ttgcagaacg
    14821 gttgtcacgt catgatggaa attgccaaac gacttcatca gccaggcgat agccgcattt
    14881 cgcgcggaat gttcaaattc ggaacgcgct accaccgtat cgtatgaaat atcagacaca
    14941 ccgcttaagc cgcgcacgac ttccagcata cgttgccgtg gtgcgcttag tcgcccttgc
    15001 aacatatcgc agaccaccag cgcaccggca ttaatgaacg gattacgcgg aataccctgt
    15061 tccatttcca gttgcactaa agaattgaac ggtgatccag acggatcttt gccgacgcgt
    15121 tgccagattt cctcttcgga gtaatgacgc atggcgacaa cgagactcag cactttggaa
    15181 atagactgaa tggaaaaacg ttcttgcgcg tctccggcct gaaaaagctg tccgtcaacg
    15241 gtacagatag caatccccaa tcgggaaccg tctactgtag ccagcgccgg aatataatcc
    15301 gcgactttac cctgaccaat gagcggccgc acttgccgca agatgttttc taaaattgca
    15361 ttatccatgg cgactgccac tttctactcc tggaccgcag gtctgaaaag acctgcgagt
    15421 atatcagagc tgaatatgtc gcgtcagatc cggtctttcc acaccgtctg gatattacag
    15481 aattcgtgta agccgaaatg ggaaagctca cgaccaaagc cactcttttt cacgccacca
    15541 aaggccactc gcgcgtcgct ggcacaataa ccattgataa acaccccacc gcattccaga
    15601 cgtgccgcca tctgtctggc ctgtgtttcg tcggtggtaa aaatggtcgc tgaaaggccg
    15661 aactcactat cattagccag ttccagcgca tgttctgcat ctttcgcaac ggtgattgcc
    15721 gcaacagggc caaacatttc ttcccgaaac gcggtcattt ctggggtaac attcgccaga
    15781 accgttggcg gatagtagtt acctgcccca gccatctttt ccccgcccag taacaaacgc
    15841 gcaccctgcg ccagggtttt ctccacctga tgatgcagct catcacgtaa atcaaaacga
    15901 gccattggtc cgagagcgtt ctcttcatca cggggatcgc ccattttcaa ggctgccgca
    15961 gctgccacaa aacgttcggt aaatgcagaa gcaattccct cttcgataat aaagcgtttt
    16021 gccgctgcac aaacctgtcc ggtattctga taacgtccgg ctaccgccgc tttaaccgcc
    16081 agttccagat cggcatcgtt aagcacaata aatggatccg aaccgcccag ttccagtacg
    16141 cattttttca gtgccgctcc agcctgtgcg ccaatagccg ctccagcacg aacacttccg
    16201 gtcaccgtga cagcagcaat gcgcgagtct ttaatcatct gactgacacc gtcgttgtcg
    16261 gcattcagcc agccatatac gccttgtggg attcccgcat ctttaaacac ctgggcaatg
    16321 agctgagcac agcccatcac attcggcgca tgtttaagta agtaaccgtt acctgcaaga
    16381 atgatgggaa ccgcgccacg catcacctgc cataacggaa agttccacgg cataatcgcc
    16441 agaatcgtcc ccaacggtcg atactcaata actgcctgct gattttccac cagcgtaggt
    16501 tccgccttca gcattgccgg accatgttct gcataccagt cacacaaatt cgccgatttc
    16561 gccacttcag cgcgcgcctg attgattggt ttgcccattt cacgggtgat catttgcgcc
    16621 atttcttcgc tacgggcgcg cagagcctta ccgatatcac gcagtttttc agcacgataa
    16681 tctatatttg tctcgcgcca gtcgcgaaag cctgctgccg ccagctgaag tgcgttttcg
    16741 atatcgttag cgccagccca cggcagcaca gaaagttgtt cacccgtggc aggatttatc
    16801 gaaattgcat gagttgccgg agtaatggtc atcggggtat ctcctttatg agtcatggta
    16861 tgaagatacg cagatttact cttgctttaa aatgaataat attaagccac ttattcacga
    16921 atcgagaata ttatggatct gacccaactg gagatgttca acgcggttgc cgaagctggc
    16981 agcataaccc aggctgcagc aaaagtgcat cgcgtgccgt cgaatctcac tacccgtcta
    17041 cgccagctgg aaacagaact gggggtagat ctgtttattc gcgagaatca gcgtttacgt
    17101 ctctctcctg ccgggcataa ctttttacgc tatagccaac aaattctcgc gttagtggat
    17161 gaagcgcgga gcgttgtcgc tggcgatgag ccgcaaggtt tattttctct tggttcgctg
    17221 gaaagcaccg cagcagtgcg cattccggcc acgctggcgg aattcaacca tcgttatccc
    17281 aaaattcagt tttcactttc caccggccct tccggcacta tgctggaagg agtgctggag
    17341 ggaaaactga atgcggcatt tattgatggg cccattaacc atactgccat tgacgggata
    17401 ccggtatacc gcgaggagct gatgatcgtc acgccacaag gtcatgcgcc agtaatccgc
    17461 gccagtcagg ttaacggcag taacatttat gccttccgcg ccaattgttc gtatcgtcgc
    17521 cacttcgaga gctggtttca tgctgacggt gccgctccgg gaactatcca tgaaatggag
    17581 tcttatcacg gaatgttggc ctgtgtggtc gcaggagcag gcattgcgct tattccacgc
    17641 tctatgctgg aaagtatgcc ggggcatcac caggttgaag cgtggccgtt agctgagcaa
    17701 tggcgttggt taacaacctg gctggtctgg cgtcgcggtg cgaaaacgcg tccgcttgag
    17761 gcatttattc aactactgga tgtgcctgac tcggcaaaac agggttacca atgagctatt
    17821 tttgatagtt cttgcagctc aaatgcccta tatagtgatg ttccgatgac ttatgactat
    17881 atggggcaaa tatggttacg ccagtaagca tcagtaatta catatctctt cctgatgatt
    17941 ttcccgtacg taatattgca cctcaggtaa aagaagtttt aaaggacttt attgatgcgc
    18001 taagtacaat aatatgtgat gaagagtggc gcacgtcttt aaacatcaac tcagccacaa
    18061 aaaagatatt taataacctt gataacttat catatattca gaggacttct tttcggggta
    18121 acgacacgct atacaatgaa aaggttcagt ttaaactcac ttatcctgcc aggaatggaa
    18181 gacacaaaga aaatattgaa tttcaggtag taataaattt aatccctatt tatttagata
    18241 attttcgcca tgatggagaa attaatattt tttgcgctcc caacccaaag cctgtcacta
    18301 tggggcgcgt atttcagacc ggcgtcgagc gtgtgctctt tctgtttctg aatgatttta
    18361 ttgaacaatt tccgatgatc aaccctggcg ttcccatcaa aagagcgcat acaccacata
    18421 ttgaacccct gcctccggat caccataccg ctgcagatta cttacgccag tttgatttgc
    18481 ttgtcctgaa ttttatctct cgcggtaatt ttgtcatact cccccgatta tggaataact
    18541 ctgaggttca cagatggttc gtcaataaag atcctaactt gatcaccgcc attctcgaca
    18601 taacagacgg cgaattaaaa gaggatttat tgcaaagcct gatggattca ttaggttcta
    18661 acaaacatgt actacccgaa gtctgcatct gctttttatc ccttttagca gaacaagaat
    18721 ctcctcattt tcaggacttg tttttatttt tcgccaatat gttattgcac tatcaccaat
    18781 ttatgaatcc caatgaaagt gatttgaatg acgtgttgat gccagcatca ttaagtgatg
    18841 ataaaattat caaacatatg gcgcgcagga ccctcaaact gtttgtaaaa aatgaaacgc
    18901 cgccaaaagt tactcacgaa gacctggtga aaaacaggcc tcgctcccct gtcagaccac
    18961 ctatacccgc aaccgccaaa acgccagacc tccctgaacg tcattaaacc gtgatgttac
    19021 cgactctctg acgcggaaca gaatcagcgt cagagaaacg gaaaacgcga tccagatcac
    19081 aaatgcattg tattcacatc attaaccgtt ttaagatcat ttcatcactt tttcgcaact
    19141 cacccgataa tctgttatga caacaaacac tgtttcccgc aaagtggcgt ggctacgggt
    19201 cgttacgctg gcagtcgccg ccttcatctt caacaccacc gaatttgtcc ctgttggcct
    19261 gctctctgac attgcgcaaa gttttcacat gcaaaccgct caggtcggca ttatgttgac
    19321 catttatgca tgggtagtgg cgctaatgtc attgcctttt atgttaatga ccagccaggt
    19381 tgaacggcgc aaattactga tctgcctgtt tgtggtgttt attgccagcc acgtactgtc
    19441 gtttttatcg tggagcttta ccgttctggt gatcagtcgc attggtgtgg cttttgcaca
    19501 tgcgattttc tggtcgatta cggcgtctct ggcgatccgt atggctccgg ccgggaagcg
    19561 ggcacaggcc ttgagtttaa ttgccaccgg tacagcactg gcgatggtct taggtttacc
    19621 tctcgggcgc attgtgggcc agtatttcgg ttggcgaatg accttcttcg cgattggtat
    19681 tggggcgctt atcacccttt tgtgcctgat taagttactt cccttactgc ccagtgagca
    19741 ttccggttca ttgaaaagcc tcccgctatt gttccgccgc ccggcattga tgagcattta
    19801 tttgttaact gtggtggttg tcaccgccca ttacacggca tacagctata tcgagccttt
    19861 tgtgcaaaac attgcgggat tcagcgccaa ctttgccacg gcattactgt tattactcgg
    19921 tggtgcgggc attattggca gcgtgatttt cggtaaactg ggtaatcagt atgcgtctgc
    19981 gttggtgagt acggcgattg cgctgttgct ggtgtgcctg gcattgctgt tacctgcggc
    20041 gaacagtgaa atacacctcg gggtgctgag tattttctgg gggatcgcga tgatgatcat
    20101 cgggcttggt atgcaggtta aagtgctggc gctggcacca gatgctaccg acgtcgcgat
    20161 ggcgctattc tccggcatat ttaatattgg aatcggggcg ggtgcgttgg taggtaatca
    20221 ggtgagtttg cactggtcaa tgtcgatgat tggttatgtg ggcgcggtgc ctgcttttgc
    20281 cgcgttaatt tggtcaatca ttatatttcg ccgctggcca gtgacactcg aagaacagac
    20341 gcaatagttg aaaggcccat tcgggccttt tttaatggta cgttttaatg atttccagga
    20401 tgccgttaat aataaactgc acacccatac ataccagcag gaatcccatc agacgggaga
    20461 tcgcttcaat gccacccttg cccaccagcc gcataattgc gccggagctg cgtaggcttc
    20521 cccacaaaat aaccgcaacc aggaaaaaga tcagcggcgg cgcaaccatc agtacccaat
    20581 cagcgaaggt tgaactctga cgcactgtgg acgccgagct aataatcatc gctatggttc
    20641 ccggaccagc ggtacttggc attgctagcg gcacaaaggc aatattggca ctgggttcat
    20701 cttccagctc ttccgacttg cttttcgcct ccggtgaatc aatcgctttc tgttgcggaa
    20761 agagcatccg aaaaccgata aacgcgacga ttaagccgcc tgcaattcgc agaccgggaa
    20821 tcgaaatgcc aaatgtatcc atcaccagtt gcccggcgta atacgccacc atcatgatgg
    20881 caaatacgta caccgaggcc atcaacgact gacgattacg ttcggcactg ttcatgttgc
    20941 ctgccaggcc aagaaataac gcgacagttg ttaatgggtt agctaacggc agtaacacca
    21001 ccagccccag gccaattgct ttaaacaaat ctaacattgg tggttgttat cctgtgtatc
    21061 tgggttatca gcgaaaagta taaggggtaa acaaggataa agtgtcactc tttagctaac
    21121 cttgcatcgc attgaccaaa acttgaaccg atttagcaaa acgtggcatc ggtcaattca
    21181 tttatttgac ttatacttgc ctgggcaata ttatcccctg caactaatta cttgccaggg
    21241 caactaatgt gaaaagtacc agcgatctgt tcaatgaaat tattccattg ggtcgcttaa
    21301 tccatatggt taatcagaag aaagatcgtc tgcttaacga gtatctgtct ccgctggata
    21361 ttaccgcggc acagtttaag gtgctctgct ctatccgctg cgcggcgtgt attactccgg
    21421 ttgaactgaa aaaagtgttg tcggtcgacc tgggagcact cacccgtatg ctggatcgcc
    21481 tggtctgtaa aggctgggtt gaaaggttgc cgaacccgaa tgataagcgc ggcgtactgg
    21541 taaaacttac caccagcggc gcggcaatat gcgaacaatg ccatcaatta gttggccagg
    21601 acctgcatca agaattaaca aaaaacctga cggcggacga agtggcaaca cttgagcatt
    21661 tgcttaagaa agtcctgccg taaacaaaaa agaggtatga cgatgtccag acgcaatact
    21721 gacgctatta ccattcatag cattttggac tggatcgagg acaacctgga atcgccactg
    21781 tcactggaga aagtgtcaga gcgttcaggt tactctaaat ggcacctgca acggatgttt
    21841 aaaaaagaaa ccggtcattc attaggccaa tacatccgca gccgtaagat gacggaaatc
    21901 gcgcaaaagc tgaaggaaag taacgagccg atactctatc tggcagaacg atatggcttc
    21961 gagtcgcaac aaactctgac ccgaaccttc aaaaattact ttgatgttcc gccgcataaa
    22021 taccggatga ccaatatgca gggcgaatcg cgctttttac atccattaaa tcattacaac
    22081 agctagttga taacgtgaca acgtcactga ggcaatcatg aaaccacttt catccgcaat
    22141 agcagctgcg cttattctct tttccgcgca gggcgttgcg gaacaaacca cgcagccagt
    22201 tgttacttct tgtgccaatg tcgtggttgt tcccccatcg caggaacaac caccgtttga
    22261 tttaaatcac atgggtactg gcagtgataa gtcggatgcg ctcggcgtgc cctattataa
    22321 tcaacacgct atgtagttat tgtcaggctc tgatcacaga gcctgacatc tccgtaatat
    22381 cttcctcaag tctcaaatat tttcgccatt gttggcgatg agatcccgat accagtaaaa
    22441 cgacgcttta cgtatccgtt ccagcgttcc cttcccttcg ttgtctttat cgacataaat
    22501 cattccgtag cgctttttca tttcccctgt tccggcagaa accaggtcaa tgcagcccca
    22561 cggggtgtag ccaattaagt caacaccatc ttcaactacg gcttttttca tttcccgaat
    22621 atgggaagca aagtaatcaa tgcgatagtg atcgttcacc gtgccgtcag cttgtctctg
    22681 gtcaaccgca ccaaatccat tttcgacaat aaacagcggc aactggaaat gatcccagaa
    22741 ccagttgagt gaataacgta gccctgccgg atcaatttgc catccccatt cggatgtatc
    22801 gatataaggg ttgctgacca aatctcgtgg ttcaacataa tccagttgcg ggttatcggg
    22861 cgaaaattgt gtcgtaaaag acatgtagta gctaaagccg ataaagtcga cacaaccact
    22921 ggcaagaatc gcgttatctt ctggtgtgat atcgaggttg aatcctttcc tggcaaagta
    22981 attcagcata tgttgcgcat aatatccacg agcatgaaca tcagtaaacc agtaacgacg
    23041 atgcattgct ttcgtggcca tcatcatatc gttgggtgca cacgtcagag gatagatggg
    23101 acacatggcg atcatacagc caatgttaaa ttcaggattg atcgattttc cagtctgtac
    23161 cgccagggca ctggcaacta actcgtaatg taccgcctgg tacattattt gttcgcgctc
    23221 attgatatct tcctctggcg aatacaagat accggaatta gtaaatggac acaggctttc
    23281 gctgaaattc acctgattat tgatttcgtt aaacgtcatc cagtacttta ctttttcttt
    23341 atagcgcgtg aagaccgttg atgcgaagcg gataaaaaag tcgatcagtt tacggtttcg
    23401 ccagccacca tattttgtca ccagatgata aggcatctca aaatgtgaaa gcgtcaccac
    23461 aggttccatt ccctgcttaa ggcattcatc gaacagatca tcataaaatt gtaaaccctc
    23521 ttcattcggc tcctgttcgt caccttgcgg aaagattcgt gtccaggcaa tggaagttcg
    23581 aaagcatttg aatcccatct cggcaaataa ctgaatatct gttttatagc gatgataaaa
    23641 atcaattgct tcatgattgg gataattaag cccgtcgata acgccttctg tcacttcacg
    23701 cggcaccccg tgagcaccag cagtcatcac atcagcgatg ctgatgcctt ttcctccttc
    23761 attccagcca ccttccaact gatgcgcggc taccgcaccg ccccataaaa aacctttttt
    23821 aaatcctgac atacacaact ccttaactaa aatagattac caccagactt ccgcctggat
    23881 gccggccata aactcgtctt tgctgttctc attaaactta aaattcgata attcgttatc
    23941 cagaatattg atatacgttc catagaagcg aatttctggt cgtgaaccta aaatgctttc
    24001 acccactttc aatgcatgcc agagtgtcgt tttataagcc gattcattta gtgttacacc
    24061 ctgctgagtt ttgttctgtt gcttaaacca gcctaattca agccccgtct gattccatgt
    24121 attccagatc caggccggtc ttattacagt gcgaatactg tcaaaatcac tatgagcacc
    24181 actttcataa ctataaacat cttcgccgtg agaatagaca agcgcgttag ccataataat
    24241 attgtcagta agatacatct cgccctgaga gattaaacgc caggcgatcc cattggtatg
    24301 atcaccatag taatagcgac catgcgccat cgtattactg gcatctgaga aactggcaaa
    24361 actgctggca taggaattat tggcaacctg taacgtaaat tcattaaacg tatcgcgttg
    24421 caggttttgc cgtaaaacag aagtcagcat ccagctatct ttcatttcaa aataactgtt
    24481 gtcattttcg ttatcttgtt gttgatccgt tttattaggt gcggaatatt tagccattaa
    24541 tgataatgtc gcatcatccc ataacgggat attgcgataa cgaacatcca cagaattggt
    24601 attcatctgg ctagtacgcg aaaaatcacg agagtaaaca tcgacatcat cacggcttaa
    24661 ggacatatca aacagcccta caccaagtgc ccagttttca atccccacac ccgcggcgac
    24721 atccgtggtt aaggttttcc agtccagcat ctggatctca tattgcggga gtttatgttt
    24781 gccgacccag aagtctgcct ctggtgcgaa gggtaaaaaa cctcgcgttg tcaggtaaat
    24841 atcgctgaac tgcatgatat tttcattggc actgtcacca aaccaggcat cgttatactg
    24901 ctcacccacg ttgccatcat aggtaacgac cgcatttgcc gttttaccgt cctgattata
    24961 aacacgctga tttaaggtca ggtcaaacca accgctcatc tcattaccaa aacgtcccag
    25021 agaacccgct gcataagttt gtggtgagcc gtgattacca gtaccccagc ctgagcggaa
    25081 gtacccctga tagctgaacc caatatcatc ctttatatat ttactgatat ctttcagggt
    25141 cacgctttcg atagtggttt tgtcgccttt actttcaata acggcaactt gctgcgatcc
    25201 atttatagta tgcgctgcgg cagacgagtt ctgttcaccg ttttcatttt tgatgttatc
    25261 agcgacggga gatgtcgctg atatttcagc aagcgaggct gatttatatt tgttttccgt
    25321 gatgcttttt tgtaaggtcg aaagtcggga tttatatact cccagttcat tctgtgttgc
    25381 tttcagctct tgtttatttt gcgacaattc attttcaagc aattcaaggc gctgctctat
    25441 ggttaatttt gttgccatcg cgttcaatgg aaacaatgct gtaataatag aaaaagacaa
    25501 aaggctgacg ttaagcgcct ttatattcat gacttatcct taagagtata ataatttcct
    25561 ccagctatcg cactggagga aaaaatcagg catggagttc ttttcttaat gaaataattt
    25621 ccctggctaa atcctggcat aatattgcat tcatcaaatg atcctgtgca tgaatcagaa
    25681 ttaaattaac ttttattttg ccacagcctt catcctgact aattaatgcg gtctgaatct
    25741 tatgtgcctg aagacaggca gattcagaac tggccattag ctgagttgcg ccctgccagt
    25801 ctttttgccc ggcacaacgt atggcttcca tcgcgtcaga acgtgcctgc cccgcgttga
    25861 ccagtaactc cataacaagt tcttcatctg caaacatcac aatctccttc ctgggtaaat
    25921 ggaattatcc gttagttgtc atgttattga ctttgttggc ggcaataaca aaaggtaaat
    25981 agatcattgt cgccacacct aaacataaaa aaccaaccag taaagccagc cagttccccc
    26041 cggttccgag gaaagcaatt aaaggtccgg gcgtagtcca gggcacggca taagcaaccg
    26101 gtggaataat ttccatcgaa acaaagaagt aaccaatggc acagttaaca atgggaacca
    26161 taataaacgg caccatcatg atggggttaa gtacaatagg aaggccaaac attatcggtt
    26221 cattgatatt gaaaataccg gggataaatg acatttttgc cagatcacgg tattccgcac
    26281 gcttagaagc gataaaaata gccaacaata accccaacgt catacctgaa ccgccgcagt
    26341 tggcgaatgc atcattaata gaagtccagg taatcggata tggcgcgccc caggtagtgc
    26401 catgttgagc ggcccaggag agattctcca gattagcctc agaaaaaacg gtttctcgaa
    26461 ttgccgatgt ggtattaggt ccgtggatcc ccagcaccca caggaaatta cccacagccc
    26521 ccaggataat taccgcaaag atattcgttc ccatatgttt gagcggcgtc tggattaatg
    26581 tgtaaataag atcgtttagt cctgccgggg atatcagtgc caggcaataa ttaagtgcgg
    26641 aaaagaacac catgacaaaa aatattggca ataaaacttt aaatgaacgc gctaccgcag
    26701 gcggtacagc tgccggcatc gttatggtga tttttgggtt tcgagctaag cgacaaaata
    26761 tttcactggt gatcaatgca acgataacag caacaaacaa cccctgcgcg ccgagatatt
    26821 tggtcgtcag gaatgcttgc ccatctatca tctgatatgg cgtataaaca ataaaaaatg
    26881 ctcctattgc cgttaatccg cataaaagat catcttgctc atagctaatc gccatattcc
    26941 tggcgaccag gaaagcaatc ataattgaca tgatatttac agaaccattc attaccggag
    27001 taaaaatagc ttgtgctttt tcaaggttgg ggaaaatgct attcagatgc agtaatccgg
    27061 caataaaacc gtctggcgat a
//
LOCUS       DAPGWD010000053        26639 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPGWD010000053 DAPGWD010000000
VERSION     DAPGWD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.053"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(61..2229)
                     /locus_tag="RHX88_002145"
     CDS             complement(61..2229)
                     /locus_tag="RHX88_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HDV2230834.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSDPTLIGSMFWVFNNIIAVLAIVWCIFISIRHVVRSGHQGQVFSV
                     GRDIVGILSFVAGFLMIVPTGNGWSLAQLIMLWGASIMGVGSANVMVQVAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGTASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQKAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYR
                     TQLQNSSYTPPLGTVVSSSEQRMANAQDPKSALSEIMGPLVSLTNRIATETSGTGTTS
                     AQINPLIKMKNIGDITMVSAEGIWTIYTTARVVVAGGKDSILVKFFNSLTGAASMLTA
                     LFEALAPPVYFLLFLMFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTTVAAIFALQAYLPDHVINFLGGRDGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKDNTKGSDDKDGIKG"
     gene            complement(2326..2910)
                     /gene="traX"
                     /locus_tag="RHX88_002146"
     CDS             complement(2326..2910)
                     /gene="traX"
                     /locus_tag="RHX88_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDV2230835.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRRIINIFVPLMETRLITNS
                     VQHMLKQQKSRLEKFRQVNNKKAQLCLSWEEALLASHMSVDDLDRRFRRRRTAWRLCC
                     WSLLAIALFLSGMLFAASSLPLMTLVRAISTLMLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            complement(2939..4141)
                     /gene="traW"
                     /locus_tag="RHX88_002147"
     CDS             complement(2939..4141)
                     /gene="traW"
                     /locus_tag="RHX88_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HDV2230836.1"
                     /translation="MQRKTLLAALIAILSGTACQAHAYSVTVVASRPVEQQVVPRMEA
                     IKGVLSDILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRNIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            complement(4108..4722)
                     /gene="traV"
                     /locus_tag="RHX88_002148"
     CDS             complement(4108..4722)
                     /gene="traV"
                     /locus_tag="RHX88_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HDV2230837.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRLFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            complement(4722..7766)
                     /gene="traU"
                     /locus_tag="RHX88_002149"
     CDS             complement(4722..7766)
                     /gene="traU"
                     /locus_tag="RHX88_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HDV2230838.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPEMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMALAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAIEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDATWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLIRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEDLASELIRKQGYNL"
     gene            complement(7856..8656)
                     /gene="traT"
                     /locus_tag="RHX88_002150"
     CDS             complement(7856..8656)
                     /gene="traT"
                     /locus_tag="RHX88_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HDV2230839.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            complement(8640..8828)
                     /gene="traS"
                     /locus_tag="RHX88_002151"
     CDS             complement(8640..8828)
                     /gene="traS"
                     /locus_tag="RHX88_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HDV2230840.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            complement(8892..9296)
                     /gene="traR"
                     /locus_tag="RHX88_002152"
     CDS             complement(8892..9296)
                     /gene="traR"
                     /locus_tag="RHX88_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HDV2230841.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVIAFLGGLIGLKKVGKQGGMGLAGC
                     ILSIVIGALLAAGPEMMSRSQKQLGISSISIG"
     gene            complement(9347..9874)
                     /gene="traQ"
                     /locus_tag="RHX88_002153"
     CDS             complement(9347..9874)
                     /gene="traQ"
                     /locus_tag="RHX88_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HDV2230842.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            complement(9874..10578)
                     /gene="traP"
                     /locus_tag="RHX88_002154"
     CDS             complement(9874..10578)
                     /gene="traP"
                     /locus_tag="RHX88_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HDV2230843.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            complement(10578..11867)
                     /gene="traO"
                     /locus_tag="RHX88_002155"
     CDS             complement(10578..11867)
                     /gene="traO"
                     /locus_tag="RHX88_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HDV2230844.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTQ
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALKNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            complement(11870..12853)
                     /gene="traN"
                     /locus_tag="RHX88_002156"
     CDS             complement(11870..12853)
                     /gene="traN"
                     /locus_tag="RHX88_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HDV2230845.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            complement(12864..13556)
                     /locus_tag="RHX88_002157"
     CDS             complement(12864..13556)
                     /locus_tag="RHX88_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HDV2230846.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            complement(13553..13900)
                     /locus_tag="RHX88_002158"
     CDS             complement(13553..13900)
                     /locus_tag="RHX88_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV2230847.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            complement(13918..17685)
                     /locus_tag="RHX88_002159"
     CDS             complement(13918..17685)
                     /locus_tag="RHX88_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV2230848.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADNSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYINKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILIDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEMR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAATPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTDTVE
                     PAVRDDPTPQQPVQSTSSSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSPETTTTT
                     PAPDSIVYAPERPDVPISLNLDEIIKSLEGEERADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATDFYGGVIELTGSDAFKQKAMQLIIEHNIKVRMKFPDQRAA
                     LEKLRKEMAVGKDTVVTHKPTPELNRNTPEQPAVPDPVKEKEATQSPASPVAPEASTV
                     STVPASSPAEPGKTADAAQGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            complement(17775..18326)
                     /locus_tag="RHX88_002160"
     CDS             complement(17775..18326)
                     /locus_tag="RHX88_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HDV2230849.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWRP"
     gene            complement(18341..18631)
                     /locus_tag="RHX88_002161"
     CDS             complement(18341..18631)
                     /locus_tag="RHX88_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV2230850.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            complement(18628..19776)
                     /gene="traJ"
                     /locus_tag="RHX88_002162"
     CDS             complement(18628..19776)
                     /gene="traJ"
                     /locus_tag="RHX88_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HDV2230851.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            complement(19773..20591)
                     /gene="traI"
                     /locus_tag="RHX88_002163"
     CDS             complement(19773..20591)
                     /gene="traI"
                     /locus_tag="RHX88_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HDV2230852.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            complement(20588..21046)
                     /gene="traH"
                     /locus_tag="RHX88_002164"
     CDS             complement(20588..21046)
                     /gene="traH"
                     /locus_tag="RHX88_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HDV2230853.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            complement(21441..22025)
                     /locus_tag="RHX88_002165"
     CDS             complement(21441..22025)
                     /locus_tag="RHX88_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDV2230854.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGEHCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            complement(22085..23287)
                     /locus_tag="RHX88_002166"
     CDS             complement(22085..23287)
                     /locus_tag="RHX88_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HDV2230855.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            complement(23373..24197)
                     /locus_tag="RHX88_002167"
     CDS             complement(23373..24197)
                     /locus_tag="RHX88_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDV2230856.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTAGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINDSSNTEVIFWHSFPFAGNTKREYYGVILRQKKEINGSVIYPALSLS
                     PTILNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            complement(24348..25502)
                     /locus_tag="RHX88_002168"
     CDS             complement(24348..25502)
                     /locus_tag="RHX88_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV2230857.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTINEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            26193..26441
                     /locus_tag="RHX88_002169"
     CDS             26193..26441
                     /locus_tag="RHX88_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230858.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(26438..>26639)
                     /locus_tag="RHX88_002170"
     CDS             complement(26438..>26639)
                     /locus_tag="RHX88_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230859.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT         6127 a         6500 c         6838 g         7174 t
ORIGIN      
        1 ccctcgtact atctattatt ttaatgccga tggggaggtt gtgcaatatt tatattgata
       61 ttatcctttt attccgtcct tgtcgtcact tccttttgta ttatctttaa gacgatctgt
      121 tttcattcca ggagtatgtc ggatattgcg acttcctcca ataaatattt ctttaacaga
      181 atttgccatg ctgccaagcg tgttggctcc atcacgtccc cctaggaagt taataacatg
      241 atcaggtagg taggcctgaa gggcaaatat ggcagctaca gtggttgtac aaattctggc
      301 gtatatcagc aagaaaccag caagactgaa aataccggtc agcgaattga actgtacgtt
      361 aaccagggca gcgccaaata atgcattcag tattgtgcct acggcgatga tggctacaga
      421 agcaaagaaa aaaccaaaga ccatgatggg cggccttatc atactatcaa ttaagtatat
      481 ataaccgtag gctgcccgac ttcctctgtc ttctgatgtc ccgagatgtg ttgcagccca
      541 cagcggcccg gcggtacatc ctattaatac gctgactatc cagttcccga tgcctgtcat
      601 ccagaaaata aacggaatga atggcaggta aattgataag gaaaatccag cacagaacat
      661 caggaacaaa agaaaataaa caggtggtgc aagtgcttca aacagcgcgg taagcatcga
      721 agcggcacct gttagtgagt tgaagaattt tacaagaatt gaatctttgc caccggcaac
      781 gacaactctg gccgttgtat atatagtcca gattccttct gcacttacca ttgttatatc
      841 tccgatattt ttcattttta tcagaggatt aatttgagca ctggttgtgc ctgtgccact
      901 tgtttccgtt gcaatcctgt tagtgagact aacaaggggg cccataattt cagacaaggc
      961 agatttcgga tcttgtgcat ttgccatgcg ttgttcactg gatgatacta ctgtgcccag
     1021 cggtggcgta taggaggaat tctgcagttg cgtcctatag gcactcatga ccgcactgaa
     1081 taagtcagta tcaccaactt ctcccagtga tgacattgaa gttacagctg gagctctgtc
     1141 agccagctct gccagacgct ggttagcagt ggcaaaggtc tgataccatg cgccaagcgt
     1201 tacccacccg taggttgtga gataagattt aagagcctct tttctggatt gctctccgtt
     1261 atcagtgggt agcgattttt gcacagcacg ttcgtattcg tcggctgctt tctgaattcg
     1321 tgtctcgata tccggcagtg ttccgttgcc agaatttctt ttttccagaa atgttgtcac
     1381 aaactcactg gcagcgttgt ccatgtcgct aatcatatta tccatggctg cacgttgtgc
     1441 tgagataaca ccgctgtact cgtttttact gaagggatta aagaattttc caatcgtcga
     1501 ctgatctgtt gttccgtttc cttctactga caggctggct gtaccacaga taccgctgcc
     1561 attggatacc gtgacagtgt aattaccact ggcggtttta gctgattctg tcatcagtga
     1621 agtcgaggat ttggcggtct gattaaaatc atttagtcct gcgttaaccg catacttaca
     1681 tagttccatt tcaaaaatgc cacgcgcagc tgttcgtgtg gatgcctgaa ctggctgtac
     1741 agtcattgaa taaccatttg cgatattatc agcggcaact tgcaccatca cattggcgga
     1801 cccaaccccc attatcgatg cgccccataa cataatgagc tgagcgagac tccagccatt
     1861 tccggtagga acgatcatca gaaaacctgc gacgaaactc agtatgccga caatatcgcg
     1921 cccgacgctg aatacctgtc cctgatgccc cgaacgcaca acatggcgga tactaatgaa
     1981 gatacaccag acgatagcaa gtacggcaat gatattgttg aacacccaga acatgcttcc
     2041 gatcagggtg gggtcgctgg ttgacagtgg attggtgacc acatcaccga atatcgtgac
     2101 cagcgcctgg cgggacagat cgtcaggatt ggtggcggca gacacaatat cctgataggt
     2161 cactgaagcc attgccggca gtgaggatat agcaaggcct gcacagagtg cgcgaagtaa
     2221 aattttcaca gtaactccgg aatagaattg cgtatgggca aaggccacac aggatttaat
     2281 taagggggat ggcgacatgc cagtggtact ggcatctgga cgtgatcagt attgtttact
     2341 ggttacggca attaatgtgg cattgcgcca gccattgcgg tcgttcagaa aatcacggaa
     2401 agtcccctgc tcgggttcgc tgacttttcg ttcatgcaac tgccagaggc ggtatgtaac
     2461 tatcagagct ctggaagcac aaagagcgac gcctgacagt atcaacataa gagtgctgat
     2521 ggcgcgtacc agtgtcatca aaggtaatga ggaggcggca aagagcatac cgctgaggaa
     2581 taaagcgatg gccagcagac tccagcagca taaccgccat gctgttctgc gccgacggaa
     2641 acggcgatcc agatcgtcta ctgacatatg gctggccagc agagcttctt cccacgagag
     2701 gcagagttgc gcctttttat tatttacctg acggaatttt tccagcctgc ttttttgttg
     2761 ctttagcata tgctgtacag aattcgttat caggcgagtc tccatcagcg gaacaaaaat
     2821 gttaataatc ctccgggtgg ctatgcgggt tatggatggt gattttttga cgtttttcgt
     2881 ctggtctgaa ttttccggta acttattcat tttttcagtc ctgttctgac gtcagctttc
     2941 aacgtgcgtt tgcctgattg attgttttct caaggctacc gagttgtttt tcataatact
     3001 ggcgggcagt cagagccagt tgctggccag agataacatt accctggcgg atctgttctt
     3061 tcagatcgtt cagctgccag ttcatttgcg cagtgattct taccagttca cggagcaggt
     3121 tatccccctg catttcctgt aaatccacca ggtatgctgt gttggcgtag cgccggccag
     3181 cctcaaaagc ttcaaactca cgggtactca tatatccccg tgcttttgct tctggtgaag
     3241 ccacctggtc gaagtaagcg gccgctgagt ctgactgcag tgtttcagcc agtgctttac
     3301 gtgtgctttc attaggcgtg ctgtcagcaa tcaacgtcag ttggggattc gacgcagaat
     3361 caatgatgcc gttgtattcg ttctggagtc cgacgtaggt tcgcccactc tgggtattca
     3421 cttctccttt acccagagca cgaccggcag aggggcggga agtgtttttc atgtaagccg
     3481 tcgctgcatc tatttgcttc tgatcccatg tcagtgccgc aggtgtgtcc gctgtacctg
     3541 cgccgtgata aatcgagcgt acctgactgt ctccacctgg tatttcgccc acagaaggac
     3601 aaacggcggt tcctccaaaa cgtgcatatt ctgcttctgt acagtaactg gcgtgtatgg
     3661 ccgcgccatc ataagcttcc cgcacgggag agtttgctgc gctggccaga cggtcgcgga
     3721 tgttacggtt gctcacaccg ccacctttac tgagttttga ggctgcggat gctgaggcac
     3781 ttttgctttc tgttgcaatc cctgatgcgg attcactgca gatggagtct ggcacggtga
     3841 agcttttccg tgcctcctcg aggcgctgag tttcattact gaaaatcatc tgctgacgtg
     3901 tcgcctgtcc gttctgggca atgacacttg ccagcttttc agagttctga ttgatagcgg
     3961 tacccgtggc tgtctgggta ctcagtatgt cactcagcac acctttgatg gcctccattc
     4021 tggggacgac ctgctgttca acggggcggc tggcgacgac ggtgactgaa tacgcatgag
     4081 cctggcatgc cgttccactc aggatggcta taagggctgc gagtaatgtt tttctttgca
     4141 ttttattgtc tcctgctcgg ggtggagcac aaggtcagga aggctgtcgt attttctgtc
     4201 ttcaggtagt ggggccagtg gaaaacactg atatcgcctg gtgatataac ctttcctgtt
     4261 ccggttatcg atgatgctga aagaacgacg tctgacgcgc cgtaaggtgg acgggtcagt
     4321 atccatgaca ggccagtcac cggcactcag tgccggcgtt ccgggaacgg atggccataa
     4381 ccaggtatca tcggacatca gcgaagccag cggtgaaggg agtatccact ggatgacatg
     4441 ctgaacgtcg aatggtgctt catccgggac agaaacctgc catattgccg ttgttatcag
     4501 ctcatctgtg ctggacggca acaacagccg catgcctgta acctttccct gtgttctggc
     4561 atccgaggcg tatatcactc tgggattggg ggccggatgt aacctgaaga gacgtgacgg
     4621 cagagtgaat gagaaactgt caccttttac ctcaagtttt ttaagaaata cctgccctct
     4681 ggcaggtctc cggcagcgtg gggtgatatg aattgtacac atcagagatt gtatccctgt
     4741 tttcggatga gctccgaggc cagatcttca ataacattgt cactgttatg ttggcccgcc
     4801 cggtgctcaa ttaatgacgt tgcagaacct ctggggaagt tttcggccag aatcttacgt
     4861 gcccgaactg aacctaactt atttgtcagc gttttacgca gggcactgtc ttttggcgat
     4921 gagttcagcg cccatagctc cagtggaccg acggtaaatt tgagtattct cgcaagcgtg
     4981 ccacttttta ccctgaatac tcccagaaca ggaacaccac taccatctgg tgccgggcct
     5041 tccggcattt tcaggaagcg tttcagcata aattcaggca cgttgaagtt atccctcagt
     5101 acggggatat cttcgggttt gtagcgtatt aaccagagag tatttgctga cttaagaacg
     5161 ctttccgggt agtccctcag atactgcgta ctcagaaccg tgcggatagc aaacttgcgt
     5221 tgttcgcgct cctgggtatc cagattttcc cagatgaagt caattccccg ggcgttatgc
     5281 agttcatcat aaacttttgt cttaacctcc tgatcaagtt ggttaatacg tttaagagcg
     5341 atttcatggt attcccgtgg aagctgcttt aacacttcat ccctgtattg tggcagggtg
     5401 aagtcaccac cggcgatctg acctgcaagc aggtacatga tgccggtttt aagcctgcct
     5461 gcaggcgttt tgtcaccggc aacattattc agatcgacag cgatgacccg ggtttccggg
     5521 ttgatcataa accgggtata cccggatatc atggggtagt cgttatgtcc ctgctccagg
     5581 gcgcggcgga tgtagtccag cagaagctcg tttgaaccat cacgctgaac agtaccgaaa
     5641 acatccctga gactggtatg gcccagcatc gaggacactt cgggaagctg gggcattgcc
     5701 tgataatgtg cccgctgagc cgccatgata tacccggcat tgtggagcat atcccggact
     5761 tcgaaccagg ttgatctggc ccaccaggtt gcatcatgtt tttcatacag tcccgagtcc
     5821 tgcagtgctg aatccaccag ttcttccgtt gaggcgcgat aaagtcgcgg attattttca
     5881 ccgtactctt taaaagccag ctcgataagc tggttgataa tttgtcgggt gtctccgggg
     5941 ttacagggtt gaccggtacc ggtatcgaca cagagagcac acagcaccga tgacatgaaa
     6001 ttcttttccg gcgtaatagg ctttttagcg ccgtacatca cgtcaaaaag gttacgggtg
     6061 tattccggat cattgctcag aataataccg acagcttcgt cttttctttc cggaggaaga
     6121 ctgtcgcgaa taagctgaac cagtccctga gcactgtatc ccttatcgat ataggcaatg
     6181 aagggcaggt ttttctgagc agatgatatc tgtatttccg acaatgtatt aatcagaact
     6241 gatttaccca gtcccggatc gcctggtgcg agttctgtat gtttattttg ttgggatgac
     6301 gccagaccaa cctcaattgc ggaaccgtct tctgtatgta acatcagatt ccctttgcct
     6361 cgccagacag agcctgccct gttaagcggg aaaagcgaga tggcatgtga caacggggga
     6421 tacaggggaa ccgggccgga gcctccggat gccgcgagaa tggtattcac ccaggcgcgt
     6481 cggggatcgc caaatgttgt cgtggtgcca catacgcccc agccttcaat ggctgatttc
     6541 agaatggcct ggtttcgtgt gcatatttca cgtgtttttc cccacgtgga ggccatgatg
     6601 gtcattatgc agacaggttc tttttcatcc gttgccgcca gggccataac ggattcatac
     6661 attggacgaa cggcagaaat gaaactgctg taagtcagaa gcgttttctt cagattaaga
     6721 gccttcatcc cgccgggcat cagatccatt ctgatgcgcc aggggactgc gcgtgggacg
     6781 gcacggacca gttcgttaaa cgtctgcaga ttttgcggag gtaaagtaat cgataccatt
     6841 ccgtgccaga gtccgcccgc ctgaaccagg ttgccctgtg tggtcacctg agtgttgaat
     6901 aactgcaaat gcaatgaagg agcatgtagt acggattcat cgtcagtcca gcgatatccc
     6961 gctggctggg catcttcagg caggtggggt tgccagttac gcggtgtgct gtgccgttct
     7021 gtctgacgac gtatttcacg cccgacctcg tgtatatcca gtagccggag aatcaggcca
     7081 tcagatgaat gtctcagtgc ctgttccaca ttatcaagaa aagcttcgtg gcggattttc
     7141 agtgcagata aggtccactg ccagggggac tgggcaaagc gtgctttcgg tacccgttca
     7201 gccagtcttc tgacaagctc atcatgggcg gtacgatcgc tgttgctgat aaggtcgggt
     7261 ccactccaga tcgccagcca gcagcgctcc cggaccagcc atggggacag tgttgtgact
     7321 ttctcatcga ccacatcctg taactggata cctgtattag ccagtgagcg tttttgtggg
     7381 gcgaccatgt cttcgatttc ttctttcccc atttcagggt cacgctcaaa aacgaagctg
     7441 attttatgac cgctgttttt atatgccgta ttcaggctgt cactcatgct ggtgatcagc
     7501 gattccagtg aacctgggcc agtctggtcg gatgcttcat ccatttccct gaaagcaccg
     7561 ctgacttcaa agacagagag atattctcca cgctttgtgg ctgcaatata gggcgaattc
     7621 agccatgggc gacgttcacg atcctgtctg tccagaccta ttacagtacg taaatcgcaa
     7681 tactcaacaa aactgcttgc gacagagaaa cgggatagcg tgttcagaca gtcttcgata
     7741 gtgaagatta aacggtttat gttcataggt gagggaactc cggacttaca ggagggaggt
     7801 ttgtgcagtt tgattttttt aatgccaccc cgcgctggag tggcatgaag aaaaatcaat
     7861 caacgtattc ttttttgatg aatgaatctg gtgtcagaat cagggcaatc ccgttcattt
     7921 tttcacgcag gggcgaacga ccggaaggtg gatagcgacg aaaaacgtca gcgaaatcgg
     7981 ccgggcggaa actgcggaat ttatcctcaa ccgccagtct gttttgcatc agtgcagaga
     8041 gcgcttcccg tccccgccgg acccggaact tatcattact gaaaacttcc tgtaatgaac
     8101 gacggttaag ggccacgacc gtggatggat gaaggtcggg tagccacacc ataacgccct
     8161 gtgctgcggc ataaccattc agtgaatgcc aggcgtggca gaaagcacat aagctgtaag
     8221 gcgggccgtc ttcatgaaac agttcctgct ctttctctga caacgactct gtactggtga
     8281 tttcagcttt ctgtacatgg cggagtgaaa gacgtctggc agacagacgg gcctgctcaa
     8341 gcgcccgttc agcggctttt actgccgtgt tgtacttttc cttttcgccg gttgcgactg
     8401 gttcaccacg cttttttaac ttatccagat gttctgtggc cttattaaga cgaactcgtg
     8461 tgtcactgat tgtgtcggta agctgtctga gtggccatga gtcatccagt ctgaaacggt
     8521 gcctgacgtt ttgccggcag cagatacatc gaccgggcgt tgttaattcc ggttccggta
     8581 acagtggctc ctgtgtatga accacatcaa tttctggaaa gggatacaac cacaagtctt
     8641 taatgctgtg ttgcatgagc aggctccgta ctggtgtaac gtttcacggc ttccagaatg
     8701 atgtttgcga tttcatcgga acagcattct gcaaaaatga attcctgatc gtcccgactg
     8761 tcgtgggcaa tcacattgcc gttttctgtt cgtcgtaacg ttatgtgctc cgggctccag
     8821 gcagacatat gtgcctcctt gtattgccga acaggaaggg caccggataa atgccagtgc
     8881 cctggacagg attagccaat ggaaattgag gaaatcccca actgtttctg gctgcgactc
     8941 atcatttcag gtccggcggc aagtagcgcc ccaatgacga tagatagaat gcaaccagcc
     9001 agtcccatcc ccccttgttt cccaactttt ttcagcccaa tcaggccccc gagaaacgca
     9061 attacgccga tgaactgcgc aatagtcagt gatgatgact gtgctgtttt ggcaccttgt
     9121 tcgacattgc gaaccatatc ggcaagatct ccatcggcaa gaacggcttt gggcagcaac
     9181 atcatgggaa tgagcagcag gaacttagtc agtttgctcc ggcatgacat tacaaacagt
     9241 tgtgcgtgga taaatgcggc gtacagacgg gccgctacga cacgaatacg tgacatagtt
     9301 tctccttaaa tggatatatc agtggtttat atgaaggtga aaaaaatcag aagacgattc
     9361 caatgcttgc cagcagggca ttgaggacac gatcgataaa gaccagcagg acgccggcaa
     9421 taaatttgac gtttccctta ctgacggatt cactggcact cagtgcggta tgtccatcga
     9481 gtccggcctt gcgccagatg gatatcccgt tcattacaaa tccaacaccg gccagcttgg
     9541 ccagcgacag aactgcattt gctgcgccag cagcagcgcc aaacgtctga ggttgtacat
     9601 atgcaatcgg gccaaatgag tcagccctga atcccatttg tacgcctccg gcgttaatca
     9661 ttgccgggag tgatacgagc atgcagctgg ttaacatccc cgcaataaat tttccgggag
     9721 acatttttac cggaccggct gaacgggcgc gggcaaacag ggtaatcatg ctgatcaccc
     9781 cgatgaattc tccccaggta aacaggaaat ccattccggc actgaaaata ctgctggcaa
     9841 agttctcaat ggcggtcagg gcatccatgt tcatcagcgt aacgtccctg cactggtgat
     9901 gatggtccgg gtagtcggat caatccgctg gatcaccaca ttgcccagcg tctgaccttc
     9961 tctgacagcc caggtactac cctgccagcg aatccaggcc ataccagttt ccatcgacat
    10021 gattttcatt ccgttaagta cagacgacgt ttttttcttt gctggatgcg gtgtggaaga
    10081 ggattcagtg acgcttttct gtgtcattat tccgttcagg cggctccgga tatcatccag
    10141 ctgacggcgt gtttcctgtg cgtattcttc atcctttttt atagcttcgc ccagcttgtt
    10201 aatgctgtcg tgaagcatat tcagtgttgc ctgaattttt tcatcgcggt tatccagctc
    10261 tgtttttaca tcccgggcaa gctggctcat atcttctcct gtccggacag tcatagtctc
    10321 agtggactga acggaaggtg atgattcttg tgtggtttgc caggtctgta cgccagcatc
    10381 cgcatcatta aacggaggca tgcctgtttc cgatggcgct ctcaggaaca gataccatat
    10441 ggctgcgagc aacacgactg cacatagccc ccatgttgct acagatatcc cccagacgga
    10501 acgtttccag aagggggcgg gtttctcttc gggttcaccg aagctgccgg tttcatctat
    10561 ttctgtttca ggtttcatta tggtgtctcc ggttgttcat cataccgacg ttgcatcaca
    10621 gcttttcttt gctccatggc tgtctgcatt cgtgcctgcg agcgttgttc cgcctgtgat
    10681 gtacgattaa ctgaagggga aatgccactg gaaagggaag atgacctggc gtcgtttgtc
    10741 attaccgggt caacaaacag aattgatacg acttcatgct gatacacttc tacctgttta
    10801 tagggctcac tttctgcctg gcgggttaat atctgacttc ctctttccgc cataccaccg
    10861 gcaataacgc ccgcaacagc ttcaccactg ggcattccca cgcgtccggt aaccgtgcca
    10921 tagttaccct gaataacctg cgtgttggca tctttataaa gtgtgccaat actgcccaca
    10981 cctcctaaca ctgacggcag aatgatatgt ttaaaccagc gtgtattcac attgctggcc
    11041 acggatgaca tcaggttgtc ttcccgttga gcatatgcat tgaccttaag gtccatgccg
    11101 ttccagctca tccggtcgaa atggatctcc acgccgttac ccaccagttt gactcccgga
    11161 ctgaacagcg ttgcccctgc ccaggggcca gcgggaatgg ttgcgataac cttggatgac
    11221 gggttatcag aatccactgc agtatcaata tatgccggta tacgggtcag ggcaggtatc
    11281 agttgtatcc ctttttctgc tacaggcgtg gacaggctgg cattctttaa tgcgaattgc
    11341 tgcgtaccat tctgccctgt tgtttctgcc ggagatttat tccacatagt cgttgctatg
    11401 gttggtgtac taccggcagg atgctgatct tttattctta caatcagctt ttgcagccgc
    11461 tccatacgtt tttcactggc tgctcgatcc tcctgtggtg tgccggaagc ctggcgatga
    11521 gcatagtttt ccggttttgc tgcaggctgt tgttgctttt cctgagtgtc cggaatatca
    11581 agcccctgtg gaagactggc aatgaacgtc tgattattgc gggctgcagc tgcggcccct
    11641 cgttcattat ccgctcttaa cagttcacga tagtgaggcg tttcggtaac gctgcgcgtt
    11701 ccgccgcttg ctgcactgtt aatgttcacg gctgactgca tatcgctgtt actccctgat
    11761 agtttggaaa acgcgatata gccgccgcca aataagatga tgctgcccag tccaagcagg
    11821 gcagccagtt tctttccgga tttaccggca tcctgttcag cactcatgat tactccagtt
    11881 ctggtgtcac gtgaatggac tggccattca cagagaaagt cagtagtggg gtgacgggaa
    11941 gtttccagag atgagttccg tcggcagagg acaaggtctg ttcaaattca tcgcgcaaca
    12001 tcgcccggga acgaaccaga aggtcatcgc cgtgttgcca gatcgttgta tccgggacat
    12061 tacctgtgaa tttcagcctc ttaactgaag ggtcccgggg aggaatacca tccagaaatg
    12121 cctgtaatgt tgcatcatgc agggcgatct tatccgtcgg gatggagaca accgggctgg
    12181 ttggtccctg ccgtggtatt cgcagatcca gacgactgtc catttcctga ctggatgacg
    12241 gcgtatctgt ttcgccactg gttacgttca ggatgaccgg taccgacaga cctttcaggt
    12301 agacagaaat atttccggat gcccatggcc gcaatggtgt gattgtcacc atgttctcgt
    12361 tgtactgcac atcaaagagt tttggtgccg cgttataagg aggatcggat tgtggccatg
    12421 gtgaaccgtt gatatcggta aatgtcacca cgctcaggtt gttcatggct gtgcgaacaa
    12481 gaggaaggct ggcaccgggg gacagattaa ctgtcagaga gctgatacgg ggtacaacac
    12541 tggtgatggg cgcattgatg gcccgctcgt tatccgccat gagactgcgt agtgagcgaa
    12601 tctcatcggc ggagagctca gaatcaagcc ttgccgcatc ctgtaccagc gggctggcct
    12661 gcccgggggc aggcagctca ccttttacca gtgttgccgg aacacctccg gtgttttggg
    12721 aggcgttctg aacgtgatcg ctggcagaat ttgtcgtttg tggtgtgcgt gcgttctgcc
    12781 atcctgcgtt atctgcagac gtggcggcgc tgcacaccag gagcagtgtg ctcagtaaat
    12841 aacgtgattg catattactt atctcaggag ttcggtccgg cgttacggga gatcatctgc
    12901 cggatttcca taccggatgg tttcagtctg gggtcaacac gctggatggt gatttcgaaa
    12961 acaaaagact gttctggctt gctggttgtc tggccgacca gacgcatacg gacaggatac
    13021 tggaatgtcc agaaccatac gccgtcggac atttgtccct gtcgtaccag aacgccagca
    13081 cctgtcgtgg cggtcagatt catcttctct ttcttgatcg tctccagaat gttagatgcc
    13141 tgtaatgcat tcacatatcc gacaaaacct tcgtcagaaa aacgcggaga cagggaggag
    13201 atctgtgaac gatagtgcac aaaatccagg ttgaatgcgg tcgccagtgc ctggctgcca
    13261 aaggccattg catcattctg actccatgct ggctgacttg ttggtgccag cctgaccaga
    13321 cgaccattgt ctgtagcgaa atattcgcgc tccacattcg ttgagtgcca gtactgaatg
    13381 gcgttaccag taatactgat aagaagacat gtgcccgtac ataaaagcgc ttttatcagc
    13441 gcagggacat tatttgcccg ctgttctgac tgcttcatag ccctgattgt gctcttcagt
    13501 atttccgggt caggtgccgg atgtgatggc gaggatggct cggtattttc ggtcataggt
    13561 tgtagtcctt ccagggatca atattccccg gcagcttttc attcaccttg tccacgaccg
    13621 cgtcgcagac ctggttttcc agctgcgaca gaatgtcgtc aaggctgggg aactggggga
    13681 ggtttatgct gatttttttg attcgtgaca gacaggactg cagggatgag gaaacatcgt
    13741 tttcacgttg tgaccatgca tccgcagctg cacgtgctcg ctcatatcct gtattactac
    13801 cggcctgtgc cgcgctaccc gcacgacagg ttgcagcact tgccgtggat gaaatcagga
    13861 taaggccgag cacggcaacc agagacttat gcatgttcat atggcttcct taccggctca
    13921 tcggccgggg ctgtgggaga gatcctgatg cagagttttt tcctgcctgt cctccggctt
    13981 ttggctgttg gaccactgct gccatgactg ctcgaagccc aggttccggt acaacgcatc
    14041 aggttgtttt tccggcgtgt agacagcagc aaaaagcggt tcttcccggg ttttcagatg
    14101 aaactgcata agctccggtg cagggcgatt cgtgctgtca gcatcgtggt ttaccgcttc
    14161 accataacct gcaaagcgta aacctttgtc ggtcagcgca ttgataacca gctgactgtt
    14221 gtcggctgat ggggtggcca gtgccggata caatgtgccg ttcagccgga ccacaccctg
    14281 cagatgttca tcggcactct ggacaagtag caggttgagt tcacgcgtat ccttatccac
    14341 tgatcccaga acaggtgtca gcttgtcagg ggctgcgctg gttatttctg ttacctgatt
    14401 tgcgttctgt ggcgcggaga ccggatgtcg gtcttcaccg ataaacagat gtgagccgac
    14461 gttttctggc atacgtaacg ctttcatctc ttcgggccac ccctggctga tttgcgcagc
    14521 cactttcata aaggtttcga ccggaatcgc ctgaatacgc tgacctttat ccggattatc
    14581 atgtgatacc gagatcccgt tggtaactga ctccatggac cacttattcc ggaaacgtgc
    14641 tttttgctct cccgggagga tgaattgttc gcggttctga agtgtcagtg tcaccacgtc
    14701 acctttctgg tggtttttga ccagactttc cagttgttcc ccccagtaga gtttgctgcc
    14761 actgcggttt ttaagctcaa caaagaaact ctctcctttg ccgggtgcat attcagcacg
    14821 accaaaattg tgcagtacgg cagtcacgct ctctcccggg cgaagttttc ctggtggttc
    14881 ttcaccttgt gccgcatctg ccgttttgcc aggttcagcc ggtgatgatg cagggacggt
    14941 gctgacggtt gatgcttcag gggctactgg ggacgccggg gattgtgtgg cttctttttc
    15001 cttaacaggg tctgggactg ctggttgttc aggggtattc cggttaagtt ccggtgttgg
    15061 cttatgggtg acaacagtat ccttaccgac agccatttcc ttgcgtaatt tttcgagcgc
    15121 cgctcgctga tccgggaatt tcatgcgcac tttgatgttg tgctcgatga tcaactgcat
    15181 cgctttttgc ttaaaggcat cactgcccgt cagctcaatg acaccaccat aaaaatcagt
    15241 ggccacagca agtgcagcca gaacactgcg atcgtcattg ctggcaccgt tgaccatttc
    15301 cagccggttt acccggtcaa tgaaggcagg tttgccatcc agtttgtaca ggacggttct
    15361 gtctgctcgt tcttcacctt ccagcgattt aatgatttcg tccagattga gcgatatcgg
    15421 tacatcaggg cgttccgggg cgtagacaat actgtcaggt gccggtgtgg tcgtcgtggt
    15481 ttccggtgag ggggtgggat cactgttctc atgcgcctgt gaatccgttt ttttacgcgt
    15541 aaaaaagcca cgcgctttgt cgagaaacga tgaagtatta tcagaagatg atgtgctctg
    15601 aacaggttgc tgtggagtgg ggtcatcccg gacagccggt tctactgtat ctgttgtggc
    15661 ctgaagttct gactcatcag cctgaacgtg gcctgtgtcc ggttgcgacg gcatatcact
    15721 gttttcctgc agatgctcag gattttcatt gttcatcagc tcctgatatg cgctgtagtt
    15781 ttcgtattct tcggggccat ctgactcagg aaggaacggc tctggctgaa ggggaatgtc
    15841 ttcagttaca tggttgaccg cctcttcccg gggagtatcc ggggatattc cggtgctttg
    15901 tttgtcatca ggctccgtca tcacggtcat ttcctctttg actgcatcag gggaaggtga
    15961 tggctcggct gattcattca tggtttcaga tgagggactg tcttcagggg atgcgggggt
    16021 ggcagcaggg gccggtattt caggtgaagt gccggattcc tgttgtgact cgtttttttc
    16081 cggttgtgac agttcggctt caaattcacg ccagtcttta tctgtggcca ccgagacaac
    16141 tttttccgga ggttccgccg gatgagcggg ttcatcaggg gcagacgccg ttgtactttc
    16201 cggggacgcc ggtgattctg caggggagtc ctgaactaca gagctttccg tttgcgcctg
    16261 aacgttatca gcgtgccgct catctttgcc gttattttca gtggcgtgac tggtaaatga
    16321 ctgtgtttca tcagcaatgt tactgctgga agcagctggt tgttcctgct gcgtggcgga
    16381 cggcagagaa ggtccctggt catggctgtg ccctgtatcg ggtgatgttt caggatattc
    16441 tgtcggagtc atggtttttt cctcagtgtg gaccggtgta aaaacgaggt tgcctatcac
    16501 cagtgcctcg cgcatttccg gggtgttact gttgggggtg gaaaccccga tgtgttctaa
    16561 ctgggcgttg atgacatgct gaaaagcggc ccgtccccgg cttgcgtcca ggtcattgaa
    16621 atcggtcagc ccctgcgctt tttcggcatc ggtgaacgca ggaaagatga ccgatcctcc
    16681 tgtcagttct gcggcttttg ttgcactgac gatacctttg ttaacccgga ttgcatgatc
    16741 gttgtcggca cagaagataa aggggctttg cgtccacttt tgccgggcat tttcggcaac
    16801 ggcaatcatg ttgcctccat ccaccgtcat cagtaccgga aggcccgtgg cttcgtgcag
    16861 ggaggccgca gtggcatagc cctcggcaaa cagtaccggc tgaccgttgc gcggtgtgcc
    16921 cagcgcaaac cagttaccgg ttttctccga atctatcagg atgcgggcat ctttcccccc
    16981 ggtgaccgga atacgctggt aagagcgtat cgcgccgtta cggttactga agggaataac
    17041 cagttcattt ttgttgttta cccgcacccc cggtgcagcc tgaatgcctt tccgggtcag
    17101 gtattcatgt gctgtggcct gcggccattt gttgatatag cggcgggcat acgcggcctg
    17161 tcggttgtac tgtgctttca gttcccgctc cgcgtcctcc ctgcgttgca ttgaatgcgc
    17221 tttcaggtgg agtcttgcgc ggggatctgt ctgttctccg ccggagaagg tccaggtgat
    17281 cggcgaatta tccgcgctgc gataatccct gtaccagccg gcaggtctgc cgtccaggaa
    17341 tccgcgatat gcgccacttt tttgtccttt tttgtcatcg gcggtcggga cacggtggat
    17401 tttcccgtcc atgaccggca gttctttcag aaccagtccg gcattctcca gaacctgggc
    17461 aaactcggtg accggatcac tgccgttcat ggagacgtcc tgtggccggg gcagccagcg
    17521 gtccagcctg gacaggtccg cccccgggcg ggcgaaccag agtttgtgct ccttgtccca
    17581 gatgacgggc gtgtgcccgt tcatcggggg atgtgctttt ttgagatctt ctatttcatc
    17641 tgcaggcacg gcaagccagg tgcggtagcg ggatacagac ggcatgagtg cttctcctga
    17701 aaaaagacgg aaaaaaacca ccgccggaag ccgggggtgg ctggggggat agaaaatcgg
    17761 gggagaaatg tttgtcaggg tctccagtct gtacccttat tccagcgatc ctgccagtgt
    17821 tcaagatatt gtgcggctac atccggcata ttcttgagca caacgacatt ctctgaattc
    17881 ttgcgtgcag cagcacgaga gaagttatag gagccagttt caaccgtgtt tccgtcaaca
    17941 atgatgactt tgtcgtgatg gatggggaag gcatctacag tccggagagg aatgtccagc
    18001 aggttgatat atttcatcgc ttcctgactt gcccggttcg tattcccctt gtcatcaaca
    18061 acaatacgga cgtccactcc gcgttttttc gcttttgcca gcgcatgcat tacatccggg
    18121 tcggtgaagg aatatgccat catcctgatg gagctttccg cactgttgat tgtgtcaagc
    18181 accagagcgg atgcgcttcc ttcaggagaa aagccgacct tcacggatgg cgtgtcgatc
    18241 attgcaaacg atggtgtcgt gaacagtaac gggcatgcca gtagcgatac ccggagcaga
    18301 gtaacgatag tttttttacc tgacataaag actccagaat ttaatcgagg ccagtccagt
    18361 ctcgtggtga ttccgtatgg tggcgatacc accatggacg accggaggcg atggcccctc
    18421 tcagaatgct gaaaatgcga gtacagatcg tcctgacagt ccagcctgaa cggtcgagaa
    18481 taacgaaaaa taaaatgatg ccggtgcaaa tccagaaggt tgttttggag ggaaaggggc
    18541 atataaacag ataaatcatg gtgaacggca gaggtacgcc ctggatgttg actggcggtg
    18601 ttgcatatcg ccagaaacct tctcttttca tgaccgtaac tcccctggcg ggataaaaag
    18661 aatcgcttct cttcggtcaa cttcattccg gatatacatt tccagaatct ggtcccggat
    18721 acggcgcttc tcctgccgga ttatggcatc aatgtgatgc ccccactctg catgcggcat
    18781 tccggaaaga ttgtcgcgaa gctcatcgtt aatgacgata tattcccgga cggctttacg
    18841 accaccgtca tttgttctga ccagaacctg aaccagaatg aactgcaaca gtgacaggac
    18901 agcccaggcc atcgaatccc tgatgacagg gggaaacagc ccaagaagac gtgccagcgt
    18961 ttcaccagga gatttcgtat gcatggtgct caggcagtaa tgcccggtgt tgcctgcctg
    19021 taccgctgcg tcggcggttt cgttatccct gatctcacca acaccgataa tttcgggatt
    19081 acgtcgtaca gcagaacgca gtccggcagc aaaactgacc acatcacgcc cgatttctgc
    19141 ctgatgcggt gggagcagat cgttttcatt tcccaggata tattccaccg ggtcttcata
    19201 tgtcacgatt ttggcgtcgg ggaaattgtc cataatgtaa cggtacagtg ctgaacacag
    19261 cgttgatttc cctgacccgg tttcaccaca tacaaatccc agaccgcttt tgcagaccat
    19321 tgcttcaagc aggtctggtt caatgttcat ggagaggatg tccggtattt ctgtcgggat
    19381 gacacgcatg gttattgata ttgctttctc ctccgcacca gatgtgccct gaatgaggtg
    19441 agagcgtaac cggatgcgtt ctccgcgttt aagaccatag cgaccggatg catccccgtt
    19501 aatctgtatc gtcctgtcaa ccggattgcc tgccagaacg cggggaatga cttcccgacc
    19561 gaacagttca tcaatcaggg aggacatgag tgtggttggt aacgttgcac tggaacaacg
    19621 cacccgtcgg ccaaagcgac tgacagatac aggtgacccg ccggtcagat ccacatcact
    19681 gactttatgt gcggcacacc agacaaagaa atgccgtagt tcatctgccg tgaaacgctg
    19741 aaacggaaaa agaccaaact catcaggaat attcatcggg agcctccttc agtgcctata
    19801 agcggacgcc agtgagattt ctgaagattg aattccgcat gatttttaag gcgacgaaca
    19861 cgatctccag tgaccagttt tttcccgtca cctgtaacgg tttgcatttg atcagaaaca
    19921 tccggacggg tgatcatgcc ctgacgccag agaagtgagt acagcatcat gccccggtaa
    19981 tcacgggtga gggttttctg gttggcttca agcgtcaggt cggcgttttg tcttccttcc
    20041 tgccatccct gccgtaatgc ggtttcccac acttttcgtt gcacgctgtc ttctggaacg
    20101 acgctgcctt ctgtacccgg agtggctgtt gttgaaagcc cccggagaag ccagtcacgc
    20161 cagcccggtg gattgctgac aaagcgttca gggcgaatga tggtccagac ccgggaagag
    20221 gtccggattt gatctggcgt gatatgagct acgtcctgtg cttcatcaat aaccggtggt
    20281 agccagcctt ccgggctaat cagtggccgg aaatcgtata aggcattaag tgtgctttcc
    20341 cgggcgttga gtgcctgaat cagctcccat gaacgctgag ccttgccacc acgaaatcca
    20401 agcgttttac ctgcgtcggt cagcattttc cagcgtgttt ctgacagact atccggcttt
    20461 tcacttcgtg gtttaagcca tgcattaatg tcaggtggtg gcgtattgac accacctggc
    20521 gagatggtag gggaagaaga acaaccagta agcagtatta ctggcaaaat gagagaggtg
    20581 agtgctttca ttttttccgg ttctccggct gcatgaagct gacctgtaac aagcccggat
    20641 attgatggat tgttgctctc caggctgtct gggcttcaat aagtctcaac gtattggaaa
    20701 acgtcatatc ccggacgtga aggttgactg gtaacggcag gcggacacca ttgtagttaa
    20761 actgcatccc cctgactctg gccagttcgt tcagtaattc aattgcatca ccttcccagt
    20821 caaaggaaag gcgatcgctg ttagctgtca gaagatgaga ccccgttgaa accggaagtt
    20881 ggccggaaaa cacacctttg tcccataatg tgtactgagt gtgccgggta accgccacat
    20941 cagaggcagc accgtagtta cgcggaacag gagtatgctg agaaggggag ttgcagccag
    21001 ccagaaaaag ggcggcaaga caccccgcag gtataatacg gttcataata attcctgagc
    21061 agttaaatca gaatgggata taccggtgct ccggtacaga caggatgatg tttgtttccg
    21121 gctatacttg cgttaccgga aataaatgcc agtgtggcca ggtaaatagc ctcatagttt
    21181 actgaaggtg attattcctg atactggctg gttgatgtgt cgtcagacat aaaacgaata
    21241 tgagcgtaat ctttaacgct ccgcgccgaa tcgatccatg atggatgact cgacgattta
    21301 tgaatcgaac gtaccggcgc ttgtccctga aacagttccc ggccctcgtc cggataacct
    21361 gcaattcaga caggcacatg tgcccgtaca ctcttgtatc ttactgagta tatcagaatt
    21421 tatgtaaatt caaatatcct ctatgcctct ttgttgtatt ttccgtcaag tatgagccgg
    21481 gtaccatcgt aatgcatgat cagggcatca aggtacgccg gtttgaattt cttgccgagc
    21541 ctatctctgg ccagaaatga taaacaatga ttatgtgtca ttattactat gttcctttta
    21601 tgagattctc ttgcgattga ttcaagcgtc ttataaagat cattattgca gtctgataaa
    21661 ctgtccataa cgacgggttc tttaccggag aagaattttg cagtttgtat ggttcttacc
    21721 gcattgctgg agtaaatatc atattcactg aagattgttg cgaacttaat accttcctgc
    21781 tgtgctttct ctgtaccggt aatagtgatt ccgcttttat ccgaatagca tggcatatct
    21841 gaacggtcgc aatgttctcc atggcggaaa aggaaaataa cggtgttatc agcattaatt
    21901 tcttttgcct gttccagggt gatatcggga agcgagcttt ttgcttttgt cactgccgct
    21961 accaggaaaa cagaaaataa aagaatgaat gctgtttttg aataagaaga acgtggtttc
    22021 atcatgttat ttaaactgaa tataaaaata cccccgggtt tagtgccggg ggcggggaca
    22081 gtaatcagaa agtgaactga agttgtgcca ttgcaccgta atcgtggtca gttcctacca
    22141 ggccggaaac atcgatatgt acgacgtcaa atggagagat accaacgcca gcggtgaagg
    22201 cgcttccgga gtttgatgcc atgttctgac gataaccggc acgcaattgc gcccatttcc
    22261 atgcgttaaa ttcagcacca acacttgcaa actgacgttt gctgtcagaa gtgaagccac
    22321 tggcaggagt cagatcaacg tcaagagcag tggtaaacag gtcattatgc caggatacac
    22381 cggcggttgc ctgtggacga actttgaagg tttctttaaa gccattgacg actttagtat
    22441 caatactgcg tggaatgatg ttctgggcaa caagaccgac agtccagttg tcattcaggt
    22501 ctgtataagc accaatatcg gcgttgaatc cgtttttcgt gttgtggtac ttgtcaccgt
    22561 caaaatcgtc tttgtcgtaa tcacgaaccg ttacgttgta gttaaacagg tccacacgct
    22621 gatattttgg tgtgacgcca agagaccatt tttgtccggc cgtttccagt tctttggcaa
    22681 atgaaattcc gacgtcagta attacagctg cacgcccgaa tgcgcgtgag gtcagggcat
    22741 tcttatcgac gtcggtaatt gttccattgg caactttgtc cagataatcc aggtctgcat
    22801 cgtttacttt tccgtttacg ctgacagttc cgtaagactt gaccataagg gcaaacggca
    22861 gagtgtcatt tgccatggct gctacagctg atacacctac ctgagcatct gcattgatat
    22921 tgcggaattc ctgcagacga tgtttaagat ttgctgctgc ctgttgtacg ccatgctggt
    22981 tatcaacagc actatcaaac aaatcccagt catctttaac atcatcagct ttatttgata
    23041 catcatcagg atcagcaact tgtgccccaa cagacggcag aagaaggctg aaatcatcat
    23101 tgctgttgtg ctttgtcagt aaagccgggt ttgccagagg tgccacacca taatgggaag
    23161 atgctacccc ggtgcccccc attgcatcgt tacgtgcttc aaaatatgac gtcgctgctg
    23221 cagcgttaaa ggaaaataat acagcagatg caatgatggt gcgggtaata tgatttttct
    23281 tcatcattta atttcctgtt tatcagaatt tagacaaaac aacgctatga cacggatgtg
    23341 ccgtaatttt ttactttggt actatctggt atttaccagg gtaataaaga cgtattaaat
    23401 aagctaacag gtagtatttg ccctgtaata aggttcccgg caaggccagg tctcaacgtg
    23461 acgcgtcgca aagacattgt tgtattctgc ctggttgtat attcagttaa ccattttctt
    23521 ttttcatcgt tcagaattgt tggtgataat gatagtgccg gatatattac agaaccattt
    23581 atttcttttt tctgtctcaa tattaccccg taatattctc ttttcgtgtt tcctgcgaag
    23641 gggaaactat gccagaaaat aacttcagtg ttgctgctat cattaataag cattctgaaa
    23701 ttatcagcag tatctggcca ttcaccggac aacgctatcg gatagttttc atggccattc
    23761 agccagtccg gattgagatt aaaacattca gcaagatgtt cgagagcgtc agctgaaaga
    23821 tagtctgcaa gtctggccgg gtcctgtata accgtggagc ggatattcca tggcgacagc
    23881 aatgttgcca gtaatggcgc atttatatca tgcgacagca tgatgtattc aattctgctt
    23941 attatgttcc ctgcggtgtt atccgcaggc ataaacatat tatgaagagc atcctcaagc
    24001 aggattcggg ttaaatcagc ggtggatatg gctaattgtt cgctcattat ccccagaact
    24061 tctcttaccc cttgggggta acggacctgc agaggtgtcg ggtttatctg acgaccgttg
    24121 atgagttttt cgccgattgc ttttttgtcc agcgagagca tcattcgggc cgcaataccg
    24181 gtcgtattca gtctcattgt tcctccgact gaaaaagtct tttcagctca tgtggttatt
    24241 gtatcttcgg tgttttttct ggtgaaacga ataatttcga tcatgcagat cgaatgtgtt
    24301 tggcgagttg ttgcaactcg cctttctgta tgtgttttga atgatgttta cagcggggtg
    24361 ctgcccgggt tcagagggca aatcataatg tagtcagggt cgttaacagg taacgctccg
    24421 ggagatggga cacgttcgcc tttctgtgct gcaatggcca gtgtacgcaa caaaacctca
    24481 ctggcgcggt gaactgcttc ctctttcgtt gtggcagtga cgtgaaggtt atcaaaatca
    24541 ccgatctcaa tacgatgcgc tttctgccca ttttcttcat tgatggtcgt gatatgggca
    24601 ggatacggca caaaccatgc tgccactttt tgtgtctgcc gccggcgggc atcaagttta
    24661 ctgaccagtt gccatgcacg aagatgagta taccgtttca gcatattcat ggaacgatgt
    24721 cctgatattg cagcaatctc cattacattc aggctaccca gttcgaagaa gcggcttatt
    24781 gcttcatgcc gtagatcgtg aaaatgcagg tcctcgatgc gaagtcgttg tgtggctatt
    24841 ctccaggcat ttttaaagcc ggatgcggtg taatcaaaaa cattgccgtg gagattaacg
    24901 ggcatcatct gaagaaagtt acgggcacgt ctggacagag gaacatcccg tgagtgaccg
    24961 tttttggttt caggtaaatg agccacaccg tggcgcaaat caatgtgctc ccaacgtaag
    25021 gccagtattt cgccctgccg catggctgtc tcaagggcaa gatggaaaat gacatacaac
    25081 atcagatttt tttcgcggaa atagcgagaa aggcgacgtt cttctgaaga cgttagccgg
    25141 cgatctcgtc cggaggatac tttcggcttg cgaaccagtt caaccgggtt agtacgacag
    25201 gttccccatt caacacgagc aatattgaac agagatgaca gaagggcgag ttcaagacgt
    25261 acagtattac ctgtaatggg tttacccgtt cgggggttga tttctgctaa acgaacgtct
    25321 ctgtatgtag caatatcaac ggttgttatt tcgtccatat tccgaagggc aatgggatat
    25381 cgcttgataa cattgctccg gtaaaactcc tgttgatgcc ctttcttgtg aacagaaact
    25441 gttttcaggt acttatccag tgcgcgtgac agggacattt tacggatgcg tggagacggc
    25501 atgctaacct caaaagaggc aaaaaataat gccaacaatc attctctgca agcgtgccaa
    25561 tccggtacct ggaaatcatc atcagcgtcg atatggacga atataaaaac atttacgtta
    25621 tatcccaaaa acacacaggt cttagggagg tttaaactgt gtattaacac ctatcgtatt
    25681 gacgggcgtg aaatggcaga aacagaagtc gttcctattg acatgcctga ctctaacggc
    25741 gagatgactt ggcaagccaa aaactacaca cagtattcgt cctactttat gaagattact
    25801 tgcttgaaat agcttattgg caaatggcat atacagttat tgaaccattc tgatcttcgg
    25861 ttgtatcgca cgcagccgac catgagttat tggctgatgg gaatgacctg gtaagccata
    25921 tgtaaccagt tcgtgaggtg catattccgc cgccacctgt aacacgtttt ccagttggac
    25981 aagtagctgt tgctccagga aagcgccaac ctgtggcctg tgctgtagca atttgaagct
    26041 caccagatcc tacctttctc caggtaccgg attggcacgt aggccagtgc caatccggta
    26101 cgtggaagac ttctggttcg ctcaatggtt cttacacaaa cttaggttca catagaggtt
    26161 cattctcagg gcggaattca gggggcagta cattgtttat ttatgcatct ggaggtaatg
    26221 gaggatctgc cggaggtgca tgtgcaaata catcccgact gcagggatat gttggtggga
    26281 cgctaattag cgtgaatgcc agcaataacc ctgcatatgg aaaaacagcc tttatcagct
    26341 ttgctgtacc tgcaggtact tcctatcaga taacatccta tccaacagaa aatacatcat
    26401 gtggtgccgg ggtattttca gtatttggat atcaaactta attgagcgtt acacacgacg
    26461 caatacaacc tgtcttatgc tcgtatttgt accatttgcg tgaacctgct gggctctcaa
    26521 ctacctggca gtagtgggca tcctctgcgt tgcccattct ggcaatggca cagaagtcga
    26581 attgccccag ataacctgtg gtggaaagct gagtaacttt gagttttcca cctattgtc
//
LOCUS       DAPGWD010000054        26146 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPGWD010000054 DAPGWD010000000
VERSION     DAPGWD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.054"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="RHX88_002171"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="RHX88_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDV2230860.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="RHX88_002172"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="RHX88_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDV2230861.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="RHX88_002173"
     CDS             complement(941..1408)
                     /locus_tag="RHX88_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDV2230862.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="RHX88_002174"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="RHX88_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDV2230863.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="RHX88_002175"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="RHX88_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDV2230864.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="RHX88_002176"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="RHX88_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDV2230865.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="RHX88_002177"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="RHX88_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDV2230866.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="RHX88_002178"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="RHX88_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDV2230867.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="RHX88_002179"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="RHX88_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDV2230868.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="RHX88_002180"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="RHX88_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDV2230869.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="RHX88_002181"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="RHX88_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDV2230870.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="RHX88_002182"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="RHX88_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDV2230871.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="RHX88_002183"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="RHX88_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDV2230872.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="RHX88_002184"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="RHX88_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDV2230873.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="RHX88_002185"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="RHX88_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDV2230874.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="RHX88_002186"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="RHX88_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDV2230875.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="RHX88_002187"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="RHX88_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDV2230876.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="RHX88_002188"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="RHX88_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDV2230877.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="RHX88_002189"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="RHX88_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDV2230878.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="RHX88_002190"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="RHX88_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDV2230879.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="RHX88_002191"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="RHX88_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDV2230880.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="RHX88_002192"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="RHX88_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDV2230881.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="RHX88_002193"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="RHX88_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDV2230882.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="RHX88_002194"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="RHX88_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDV2230883.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="RHX88_002195"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="RHX88_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDV2230884.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="RHX88_002196"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="RHX88_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDV2230885.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="RHX88_002197"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="RHX88_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDV2230886.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="RHX88_002198"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="RHX88_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDV2230887.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="RHX88_002199"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="RHX88_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDV2230888.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="RHX88_002200"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="RHX88_002200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDV2230889.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="RHX88_002201"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="RHX88_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDV2230890.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="RHX88_002202"
     CDS             15548..15916
                     /locus_tag="RHX88_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDV2230891.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="RHX88_002203"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="RHX88_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDV2230892.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="RHX88_002204"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="RHX88_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDV2230893.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="RHX88_002205"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="RHX88_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDV2230894.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="RHX88_002206"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="RHX88_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDV2230895.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="RHX88_002207"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="RHX88_002207"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDV2230896.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="RHX88_002208"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="RHX88_002208"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDV2230897.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="RHX88_002209"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="RHX88_002209"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDV2230898.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="RHX88_002210"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="RHX88_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV2230899.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /locus_tag="RHX88_002211"
     CDS             22593..23066
                     /locus_tag="RHX88_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDV2230900.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="RHX88_002212"
     CDS             23095..23637
                     /locus_tag="RHX88_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDV2230901.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="RHX88_002213"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="RHX88_002213"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDV2230902.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="RHX88_002214"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="RHX88_002214"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDV2230903.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="RHX88_002215"
     CDS             25034..25291
                     /locus_tag="RHX88_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDV2230904.1"
                     /translation="MNQALQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="RHX88_002216"
     CDS             complement(25267..25821)
                     /locus_tag="RHX88_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDV2230905.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6712 a         6204 c         6784 g         6446 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gctagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggtaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacaacgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatttatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgctgaag aatgccggtt gcttaccgcc
    15721 agaacttgag caacggagag aagcaattca gcttctggat atcctgagag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ttgagtaccg attttttacg cggcgattat gctgacaagt tgttggacaa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc tgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg cgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgca
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgttc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actggcagga ttacactcgc
    16381 gccgtaaaat aaccaattgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg cgtagagaaa
    16501 aataagaagg ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgtt
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgctggtgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgccaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcgcggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 acgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga tgctttccag
    20101 actttaaccg gctgtccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac cgacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcaa cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccgtctg
    21001 cttccaattc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttctggtg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacggtta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tctgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcaaattcag gtgcttaacc ttgaaacccg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccttg catgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtt gggtgcgatc ctgatagcga
    23761 aacagcagtg atgagactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattctgctc atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactgccac ttctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccctcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgaa
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaag tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacaag
    26041 atggtcgtaa caacgaagag aaaaaatcct gaaattcagg gttgactctg aaagaggaaa
    26101 gcgtaatata cgccacctcg cgacagtgag ctgaaagctg cgtcgc
//
LOCUS       DAPGWD010000055        26040 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPGWD010000055 DAPGWD010000000
VERSION     DAPGWD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.055"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..1747)
                     /locus_tag="RHX88_002217"
                     /pseudo
     CDS             complement(1..1747)
                     /locus_tag="RHX88_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2271..4199
                     /gene="thrS"
                     /locus_tag="RHX88_002218"
     CDS             2271..4199
                     /gene="thrS"
                     /locus_tag="RHX88_002218"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDV2230906.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4203..4745
                     /gene="infC"
                     /locus_tag="RHX88_002219"
     CDS             4203..4745
                     /gene="infC"
                     /locus_tag="RHX88_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDV2230907.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4842..5039
                     /gene="rpmI"
                     /locus_tag="RHX88_002220"
     CDS             4842..5039
                     /gene="rpmI"
                     /locus_tag="RHX88_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDV2230908.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5092..5448
                     /gene="rplT"
                     /locus_tag="RHX88_002221"
     CDS             5092..5448
                     /gene="rplT"
                     /locus_tag="RHX88_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDV2230909.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5565..5689
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5571..5615
                     /gene="pheM"
                     /locus_tag="RHX88_002222"
     CDS             5571..5615
                     /gene="pheM"
                     /locus_tag="RHX88_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDV2230910.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5899..6882
                     /gene="pheS"
                     /locus_tag="RHX88_002223"
     CDS             5899..6882
                     /gene="pheS"
                     /locus_tag="RHX88_002223"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDV2230911.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6897..9284
                     /gene="pheT"
                     /locus_tag="RHX88_002224"
     CDS             6897..9284
                     /gene="pheT"
                     /locus_tag="RHX88_002224"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDV2230912.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9289..9588
                     /gene="ihfA"
                     /locus_tag="RHX88_002225"
     CDS             9289..9588
                     /gene="ihfA"
                     /locus_tag="RHX88_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDV2230913.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9689..10669
                     /gene="btuC"
                     /locus_tag="RHX88_002226"
     CDS             9689..10669
                     /gene="btuC"
                     /locus_tag="RHX88_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDV2230914.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10732..11283
                     /gene="btuE"
                     /locus_tag="RHX88_002227"
     CDS             10732..11283
                     /gene="btuE"
                     /locus_tag="RHX88_002227"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDV2230915.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11283..12032
                     /gene="btuD"
                     /locus_tag="RHX88_002228"
     CDS             11283..12032
                     /gene="btuD"
                     /locus_tag="RHX88_002228"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDV2230916.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12110..12574
                     /locus_tag="RHX88_002229"
     CDS             12110..12574
                     /locus_tag="RHX88_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2230917.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12821..13534
                     /gene="ydiV"
                     /locus_tag="RHX88_002230"
     CDS             12821..13534
                     /gene="ydiV"
                     /locus_tag="RHX88_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDV2230918.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13597..15033
                     /locus_tag="RHX88_002231"
     CDS             13597..15033
                     /locus_tag="RHX88_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDV2230919.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(15037..15228)
                     /gene="hemP"
                     /locus_tag="RHX88_002232"
     CDS             complement(15037..15228)
                     /gene="hemP"
                     /locus_tag="RHX88_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDV2230920.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15360..16406)
                     /gene="aroH"
                     /locus_tag="RHX88_002233"
     CDS             complement(15360..16406)
                     /gene="aroH"
                     /locus_tag="RHX88_002233"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDV2230921.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16563..17396)
                     /gene="ppsR"
                     /locus_tag="RHX88_002234"
     CDS             complement(16563..17396)
                     /gene="ppsR"
                     /locus_tag="RHX88_002234"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDV2230922.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17729..20107
                     /gene="ppsA"
                     /locus_tag="RHX88_002235"
     CDS             17729..20107
                     /gene="ppsA"
                     /locus_tag="RHX88_002235"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDV2230923.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(20164..21810)
                     /gene="fadK"
                     /locus_tag="RHX88_002236"
     CDS             complement(20164..21810)
                     /gene="fadK"
                     /locus_tag="RHX88_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDV2230924.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSVLRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(21867..22160)
                     /locus_tag="RHX88_002237"
     CDS             complement(21867..22160)
                     /locus_tag="RHX88_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV2230925.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(22157..23446)
                     /locus_tag="RHX88_002238"
     CDS             complement(22157..23446)
                     /locus_tag="RHX88_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV2230926.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPKMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(23502..24440)
                     /locus_tag="RHX88_002239"
     CDS             complement(23502..24440)
                     /locus_tag="RHX88_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDV2230927.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(24460..25224)
                     /locus_tag="RHX88_002240"
     CDS             complement(24460..25224)
                     /locus_tag="RHX88_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDV2230928.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            25540..>26040
                     /locus_tag="RHX88_002241"
     CDS             25540..>26040
                     /locus_tag="RHX88_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDV2230929.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGAARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKN"
BASE COUNT         6497 a         6209 c         6768 g         6566 t
ORIGIN      
        1 acatgttttc atgattttta gcggccaaca gtgtgaataa ttgacgttga ctaaaagaat
       61 atttttttgc aaaagtactc agtgttgata tataagataa cacgacgtct ttattaccct
      121 tgctcatggc gatgtataaa gcaggggttc catgtgctag ctttgctcaa cttgtattta
      181 aaggcgatgc cattaatttt agagaggaac cgtgtgacac acaaagggtg attattttcc
      241 attgcggcgt ataatcctgg ctcaccatca tgtattttca tggctaaaat actcatcgcc
      301 agttttttgt ttaaaagctc ttgtgctatt ggagtggtta atgcattcag aaagatatcg
      361 attatttcat cgaatccata caacatggag atataaagtc caggtgtttc gttagcactt
      421 ttagtttgca gtaatttaac cagattatct tcatgaatga tgtgattatc taccagtgac
      481 tgaatttctt gcataaatat tttcacgttg tcagtatgtc tgttcatcat agccaaaaat
      541 aaagcaggtg taccaacgct attctgcgca gttattattt caatgatttc tttttcaggg
      601 agtatgggca actgtttgaa taatgtaata agctgttgag tctggcctgt agccattatt
      661 aacaacattg ccgatggatg tataggggca aatggcattc gtgatgcaat acccgcgaaa
      721 atagcccctg gaactcccac aggtaatagg ggcagaatag caattgcaca gttgcgaaca
      781 tacctatcag cccaggtctg agcatcggga cctgaactga tgaacttatc aagcgaaaga
      841 aagccccgtg ctgtcctgtg ggtgtctctg acggcaacgt tggtatcgtt cgggtcgtaa
      901 acggacacaa catagtttaa ttgtccatac actgactgct ttattcgtag ccttactgac
      961 aaagtatgat tgtctatata taaaatcgcg gacatatgac tgacattgtt tgccatcatt
     1021 cgacaaaaga aaatatctaa agcgaagcca aaatccttac tcgcgataac gcgactccct
     1081 gcaggaggat ggcgaagtaa tgctctgaac tccgcgtccc ggactaaggg gacatgctgc
     1141 tgtatggatt caacggagga gtaatgttta atcaggttga ctttgccttt tccatttctc
     1201 aatgaatcaa gggcgtattg caatgacaga tggcgacaaa aaatagcgtc agaaggagat
     1261 tgcaaaaaac atgcactacc attgagattt actgttgggt gtatagtttt ttctctatgg
     1321 atttttttag acagtttcat acaatggtta cgatcttcag cagttaactg ttcctgttca
     1381 atataatcca gtatgcttcg caaatggtta gctttaatag gcgtggtttc cagtaataag
     1441 gaaatgaaat ctttttatag tgtaatgtaa tgagtatatt ttctgcatgg atgggtgaag
     1501 tggcaaagtt aatggtaaaa agggcagtgg aataacaatt ttgtgatatt tttcgacagt
     1561 ttgcggtaaa ttcctgaata acacagtatg acagcatcga acttaagtca gaggtatcaa
     1621 gtagggtgat gagctggcgc gcaaatttat taataggctc tgatgaaggt gttggtatgt
     1681 cgctatcgat gtgcaatcta tgaggagact tataatgagt tctgataatg atatcgtttt
     1741 gactcataat ctattcgcct tatatatatc tggaggtttt gaggatgatg attaattatt
     1801 atcgccagaa cgaaatgtgc acgaaaaatt gttataagaa ttgtgtcact tttatcattg
     1861 tgatatgacc acttcgtcat taatctcatg agcgtttaaa atactaccgc caaatcacgc
     1921 aacgaacttt gctggattca gtttatgctg ctgtaaatcc gctcgcagta aacctttcag
     1981 acgcaccgtg atgttatcag tggttcttta agcgttttgc tggtgtactc actacaaacg
     2041 aattgcgaat cgaatcaatg tgaaacggaa aggtacaatc tccctttttt tagtgttaac
     2101 atcgctcaac cgggtggaag gtgagaggtt aatgtctttc atttgtgtac ctgattgcga
     2161 accaatttag catttgttgg ctagatggtt tcgcaatgaa ctgttaataa acaaattttt
     2221 ctttgtatgt gatctttcgt gtgggtcacc actgcaaata aggatataaa atgcctgtta
     2281 taactcttcc tgatggcagc caacgccatt acgaccacgc tgtaagcccc atggatgttg
     2341 cgctggacat tggtccaggt ctggcgaaag cctgtatcgc agggcgcgtt aatggcgaac
     2401 tggttgatgc ttgcgatctg attgaaaacg acgcacaact gtccatcatt accgccaaag
     2461 acgaagacgg tctggagatc attcgtcact cctgtgcgca cctgttaggg cacgcgatta
     2521 aacaactttg gccgcatacc aaaatggcaa tcggcccggt tattgacaac ggtttttatt
     2581 acgacgttga tcttgaccgc acgttaaccc aggaagatgt cgaagcactc gagaagcgga
     2641 tgcatgagct tgctgagaaa aactacgacg tcattaagaa gaaagtcagc tggcacgaag
     2701 cgcgtgaaac tttcgccaac cgtggggaga gctacaaagt ctccattctt gacgaaaaca
     2761 tcgcccatga tgacaagcca ggtctgtact tccatgaaga atatgtcgat atgtgccgcg
     2821 gtccgcacgt accgaacatg cgtttctgcc atcatttcaa actaatgaaa acggcagggg
     2881 cttactggcg tggcgacagc aacaacaaaa tgttgcaacg tatttacggt acggcgtggg
     2941 cagacaaaaa agcgcttaac gcttacctgc agcgcctgga agaagccgcg aaacgcgacc
     3001 accgtaaaat cggtaaacag ctcgacctgt accatatgca ggaagaagcg ccgggtatgg
     3061 tattctggca caacgacggc tggaccatct tccgtgaact ggaagtgttt gttcgttcta
     3121 aactgaaaga gtaccagtat caggaagtta aaggtccgtt catgatggac cgtgtcctgt
     3181 gggaaaaaac cggtcactgg gacaactata aagatgcaat gttcaccacg tcttctgaga
     3241 accgtgaata ctgcattaag ccgatgaact gcccgggtca cgtacaaatt ttcaaccagg
     3301 ggctgaagtc ttatcgcgat ctgccgctgc gtatggccga gtttggtagc tgccaccgta
     3361 atgagccgtc aggttcgctg catggcctga tgcgcgtgcg tggatttacc caggatgacg
     3421 cgcatatctt ctgtactgaa gaacaaattc gcgatgaagt taacggatgt atccgtttag
     3481 tctatgatat gtacagcact tttggcttcg agaagatcgt cgtcaaactc tccactcgtc
     3541 ctgaaaaacg tattggcagc gacgaaatgt gggatcgtgc tgaggcggac ctggcggttg
     3601 cgctggaaga aaacaacatc ccgtttgaat atcaactggg tgaaggcgct ttctacggtc
     3661 cgaaaattga atttaccctg tatgactgcc tcgatcgtgc atggcagtgc ggtacagtac
     3721 agctggactt ctctttgcct tctcgtctga gcgcttctta tgtaggcgaa gacaatgaac
     3781 gtaaagtacc ggtaatgatt caccgcgcaa ttttggggtc gatggaacgt ttcatcggta
     3841 tcctgaccga agaattcgct ggtttcttcc cgacctggct tgcgccagtt caggttgtta
     3901 tcatgaatat taccgattca cagtctgaat acgttaacga attgacgcaa aaactatcaa
     3961 atgcgggcat tcgtgttaaa gcagacttga gaaatgagaa gattggcttt aaaatccgcg
     4021 agcacacttt gcgtcgcgtc ccatatatgc tggtctgtgg tgataaagag gtggaatcag
     4081 gcaaagttgc cgttcgcacc cgccgtggta aagacctggg aagcatggac gtaaatgaag
     4141 tgatcgagaa gctgcaacaa gagattcgca gccgcagtct taaacaattg gaggaataag
     4201 gtattaaagg cggaaaacga gttcaaacgg cgcgccctaa ccgtatcaat ggcgaaattc
     4261 gcgcccagga agttcgctta acaggtctgg aaggcgagca gcttggtatt gtgagtctga
     4321 gagaagctct ggagaaagca gaagaagccg gagtagactt agtcgagatc agccctaacg
     4381 ccgagccgcc ggtttgtcgt ataatggatt acggcaaatt cctctatgaa aagagcaagt
     4441 cttctaagga acagaagaaa aagcaaaaag ttatccaggt taaggaaatt aaattccgtc
     4501 ctggtacaga tgaaggcgac tatcaggtaa aactccgcag cctgattcgc tttctcgaag
     4561 agggtgataa agccaaaatc acgctgcgtt tccgcggtcg tgagatggcg caccagcaaa
     4621 tcggtatgga agtgcttaat cgcgtgaaag acgatttgca agaactggca gtggtcgaat
     4681 ccttcccaac gaagatcgaa ggccgccaga tgatcatggt gctcgctcct aagaagaaac
     4741 agtaaggcct tcaagtagca ctgtctgtgg agccttcggg cttcacaggc tttgttcgcc
     4801 tttgtttcgt ttattaacaa tgcgaagtgg aagttattaa aatgccaaaa attaagaccg
     4861 tacgcggtgc tgctaagcgc ttcaaaaaaa ccggtaaagg tggttttaag cacaagcacg
     4921 ctaacctgcg tcacattctg accaaaaaag cgaccaaacg taaacgtcac ctgcgtccga
     4981 aagccatggt ttccaaaggc gatctgggcc tggtaatcgc gtgcctgccg tacgcataag
     5041 ccgttaacgt ttttaacttt ttaattagaa tatagataca ggagagcaca tatggctcgc
     5101 gtaaaacgtg gtgttattgc acgcgcacgt cacaagaaaa ttttgaaaca agctaaaggc
     5161 tactacggtg cgcgttctcg cgtataccgc gttgccttcc aggctgttat caaagctggt
     5221 cagtatgctt accgtgaccg tcgtcaacgt aagcgtcagt tccgtcaact gtggattgcg
     5281 cgtatcaacg cagcagcacg tcagaacggt atttcttaca gcaaattcat caatggcctg
     5341 aaaaaagcct ctgttgaaat cgaccgtaag atcctggctg atatcgcagt attcgacaaa
     5401 gtagcgttca ccgctctggt tgaaaaagcg aaagcagctc tggcataagc cagttgaaag
     5461 agggagctag tctccctctt ttcgtttcaa cgccatcaaa acattgactt ttatcgccgt
     5521 agccttttca ataaaggtct tttgaagagt accaaaaggt aacgcaagca atgaatgctg
     5581 ctattttccg cttctttttt tactttagca cctgaatcca ggaggctagc gcgtgagaag
     5641 agaaacggaa aacagcgcct gaaagcctcc cagtggaggc tttttttgta tgcgcgtttt
     5701 gaaaaataaa aagcctgtct catcaggtgt aattgtcgca gccagtctgg acacggccag
     5761 cgcggagaaa ccggagcgta tttaagtacg tgagaatttc gagcactgcc cgggaccaaa
     5821 atggcaagta aaatagcctg atgggatagg ctctaagtcc aacgaaccag tgtcaccact
     5881 gacacaatga ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc
     5941 attagccagg cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa
     6001 aaagggcact taacccttca gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg
     6061 gcagctggtg cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt
     6121 aaagcggaac tggaaagcgc agcgctgaat gcgcgtctgg cggcggaaac gattgatgtc
     6181 tccctgccag gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg taccatcgac
     6241 cgtatcgaaa gtttcttcgg tgagcttggc tttactgtgg caaccgggcc ggaaatcgaa
     6301 gacgattatc ataacttcga tgctctgaac attcctggtc accacccggc gcgcgctgac
     6361 cacgacactt tctggtttga cactacccgc ctgctgcgta cccagacctc tggcgtacag
     6421 atccgcacca tgaaagccca gcagccaccg attcgtatca tcgcgcctgg ccgcgtttat
     6481 cgtaacgact acgaccagac tcacacgccg atgttccatc agatggaagg tctgattgtt
     6541 gataccaaca tcagctttac caacctgaaa ggcactctgc acgatttcct gcgtaacttc
     6601 tttgaggaag atttgcagat tcgcttccgt ccttcctact tcccgttcac cgagccttct
     6661 gcagaagttg acgtcatggg taaaaacggt aaatggctgg aagtgctggg ttgcgggatg
     6721 gtgcatccga acgtgctgcg taacgttggc atcgacccgg aagtttactc tggtttcgcc
     6781 ttcgggatgg ggatggagcg tctgactatg ttgcgttacg gcgtcaccga cctgcgttca
     6841 ttcttcgaaa atgatctgcg tttcctcaaa cagtttaaat aaggcaggaa tagattatga
     6901 aattcagtga actgtggtta cgcgaatggg tgaacccggc gattgatagc gatgcgctgg
     6961 cgaatcaaat cactatggcg ggcctggaag ttgacggtgt agaaccggtt gccggtagct
     7021 tccacggcgt ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac gctgacaaac
     7081 tgcgtgtgac aaaagtgaat gttggcggcg atcgcctgct ggacatcgtc tgcggtgcgc
     7141 caaactgccg tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt ctgccgggtg
     7201 atttcaaaat taaagcggcg aaactgcgtg gtgaaccgtc tgaagggatg ctgtgctcct
     7261 tttctgagct gggaatttct gacgatcata gcggcattat cgagctgcct gcggatgcgc
     7321 cgattggcac cgacatccgt gaatacctga aacttgatga caacaccatc gaaatcagcg
     7381 tgacgccaaa ccgtgccgac tgtttaggta tcattggtgt tgcgcgtgac gttgccgtgc
     7441 tgaatcagct gccgctggtt gaaccggaaa tcgttccggt tggtgcgacc atcgacgaca
     7501 cgctgccgat tacagtcgaa gcgccggaag cctgcccgcg ttatcttggc cgtgtggtaa
     7561 aaggcattaa cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg
     7621 ggatccgttc tatcgatgca gttgttgacg tcaccaacta tgtactgctc gaactgggcc
     7681 agccgatgca cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg cggatggcga
     7741 aagagggcga aacgttggta ctgcttgatg gcactgaagc gaagctgaat gctgacactc
     7801 tggtcatcgc cgaccacaac aaggcgctgg cgatgggcgg catctttggt ggcgaacact
     7861 ctggcgtgaa tgacgaaacg caaaacgtct tgctggaatg cgctttcttc agcccgctgt
     7921 ctatcaccgg tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac cgttatgagc
     7981 gtggcgttga tccggcactg cagcacaaag cgatggaacg tgcgacccgt ctgctgatcg
     8041 acatctgcgg tggtgaggct ggtccggtaa ttgatatcac caacgaagca acgctgccga
     8101 agcgtgcaac catcacttta cgtcgtagca aactggatcg cctgatcggc catcatattg
     8161 cggatgagca ggtaactgac attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag
     8221 acgagtggca ggcagttgcg ccgagctggc gtttcgatat ggagattgaa gaagatctgg
     8281 tcgaagaagt cgcgcgtgtt tacggctaca acaacatccc ggatgagccg gtacaggcaa
     8341 gcctgattat gggtactcac cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc
     8401 tcaacgacaa aggctatcag gaagtgatca cctacagctt cgttgatccg aaagtgcagc
     8461 agatgatcca tccaggcgtt gaagccttac tgctgccaag cccgatctct gttgaaatgt
     8521 cagcaatgcg tctttctctg tggaccggcc tgctggcaac cgtggtgtac aaccagaacc
     8581 gtcagcagaa ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca gatactcagg
     8641 caccgttggg cattcgtcag gatctgatgt tagccggtgt gatttgcggt aaccgttacg
     8701 aagagcactg gaacctggca aaagagaccg ttgatttcta tgatttgaaa ggcgatcttg
     8761 aatccgttct cgacctgacc ggtaaactga atgaggttga gttccgtgca gaagcgaatc
     8821 cggcactgca tccggggcaa tccgcagcga tttatctgaa aggtgaacgt attggttttg
     8881 ttggggttgt tcatcctgaa ctggaacgta aactggatct taacggtcgc actctggtgt
     8941 tcgaactgga gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc
     9001 gcttcccggc gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg
     9061 atattttatc cgaatgtaag aaagttggcg taaatcaggt agttggcgta aacttatttg
     9121 acgtgtaccg cggtaagggt gttgcggagg ggtataagag cctcgccata agcctgatcc
     9181 tgcaagatac cagccgtaca ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg
     9241 tagaggcatt aaaagagcga ttccaggcat cattgaggga ttgaacctat ggcgcttaca
     9301 aaagctgaaa tgtcagaata tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa
     9361 gaactggttg aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg
     9421 aaactctctg gttttggtaa cttcgatctg cgtgataaga atcaacgccc gggacgtaac
     9481 ccgaaaacgg gcgaggatat tcccattaca gcacggcgcg tggtgacctt cagacccggg
     9541 cagaagttaa aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc tgatctaact
     9601 aaaaaggccg ctctgcggcc ttttttcttt tcactgtcga agagtcaccg taaaatcaac
     9661 gccatgacac ttcagcagaa cggataccat gctgacactt gcccgccaac aacagcgaca
     9721 aaatattcgc tggttattat gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct
     9781 ttgcgccggt gaacaatgga tctcgccagg tgactggttt tctcctcgtg gcgaactgtt
     9841 cgtctggcag attcgcctgc cacgtacgct ggctgtattg ctggttggtg cggcgctggc
     9901 tatatccggc gcagtaatgc aggcgttgtt tgaaaatcct ctggcagaac ctggactact
     9961 tggcgtctct aacggcgcag gcgtagggct tatcgccgcg gtattgcttg ggcaagggca
    10021 actcccaaac tgggcactag ggctgtgtgc gattgctggc gcgcttatca tcactttaat
    10081 actcttacgt ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc
    10141 attagggatt atctgtagcg cactaatgac gtgggctatc tacttttcca cctctgttga
    10201 tttacgtcag ctgatgtact ggatgatggg cggttttggc ggcgtagact ggcggcaaag
    10261 ctggctgatg ctggcattga tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat
    10321 gaatatgtta gcacttggcg agatctcggc gcggcaactg ggtttacccc tgtggttctg
    10381 gcgcaatgtg ctggtggcag cgaccggctg gatggttggc gtcagtgtgg cgctggcggg
    10441 tgctatcggc tttattggtc tggtgatccc acatattctt cggttgtgtg gtttaaccga
    10501 tcatcgcgta ttacttcccg gctgcgcgct ggcaggggcg agcgcattgc tgctggccga
    10561 tgttgtagcg cgcttggcat tagctgccgc agagctgcct attggcgtgg tcaccgcaac
    10621 gttgggtgcg ccggtgttta tctggttatt gttaaaagca ggacgttagc cgcaaaaaga
    10681 cggtctatga ttaaaagcta agatttttac tgaccacacc caggagaaac gatgcaagat
    10741 tccattctga cgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac
    10801 gccggtaatg tgctgttgat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat
    10861 gagcagttgg agaatattca gaaagcctgg gccgatcgag gttttgtggt gctgggattc
    10921 ccgtgcaacc agtttctgga acaagaaccg ggcagcgatg aagagattaa aacttactgt
    10981 accaccacat ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa tggcgaagga
    11041 cgccatccgc tgtatcaaaa attgattgcc gcagcgccga ccgcagtcgc gccggaagag
    11101 agcggattct atgcccgtat ggtcagcaaa ggtcgtgcac cgctgtaccc tgatgatatt
    11161 ttatggaatt ttgagaaatt tttagttggc agggacggat tggtcatcca gcgtttttcc
    11221 ccggatatga cgccggaaga ccccattgtg atggaaagca ttaaactggc gttggcaaaa
    11281 taatgtctat tgtgatgcag ttacaggatg ttgcggaatc tacccgcctg gggccacttt
    11341 ctggcgaggt tcgggctggg gagatcctgc atctggtggg gccgaatggc gcgggtaaga
    11401 gtaccttgct tgcacgaatg gccggaatga ccagcggtaa gggaagcatt cagttcgcgg
    11461 ggcaacccct ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac
    11521 aacagcagac gccgccgttt gcaatgccgg tctggcacta cctgacactg catcagcacg
    11581 ataaaacgcg taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac
    11641 tcggacgtag caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg
    11701 tggtgctgca aatcacacca caagccaatc ccgcaggtca attgctgctt cttgatgagc
    11761 cgatgaacag tcttgatgtt gcgcaacaaa gtgcgttaga caaaattctg agcgctctgt
    11821 gtcagcaagg actggcgatt gtgatgagca gtcacgatct caaccacaca ttgcgtcatg
    11881 cgcatcgggc gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agagaagagg
    11941 tgctcacgcc ggcaaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg
    12001 aaggtcacag aatgctgatt tcgaccatct gatttcatta gtatcttgaa aatcgggtaa
    12061 ttacgacgct aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg
    12121 ccttattttg atcacagcac tgtttctggc cgggtgtagc caccataaag caccgccgcc
    12181 aaatgccaga ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag
    12241 ctggcatggc acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg
    12301 atttgtggtt gtaacgatgc gcgatcgttt cgatttgcag ctgccccgag aaaccaaaga
    12361 acaagcctct atcggcacgc aaattgataa agacgagttg ctgcctggtg acctggtctt
    12421 tttcaaaacg gggtccggac aaaatggttt gcatgtaggt atttacgata ccaacaacca
    12481 atttatccac gcctctacca gcaagggagt gatgcgttcc tcacttgata atgtctattg
    12541 gcagaaaaat ttctggcagg cgagacgaat ctagtatgtc gaaaaatgga cggcgatgat
    12601 acatcgccgt ccatttactc ctgaatacag tagtattttg cagacaaaaa ttccgtaaat
    12661 ggattattcg tttataatca ggataatcta aaaaaaggac ccctgaaaaa gtggttaaca
    12721 tggaacaaat gaaagttact ccttctgtaa ttgttatgtc ttatggcctc atccctttta
    12781 gccggatact gaaaaacatc cttcgagagg gacggttacc atgaagattt ttttggagaa
    12841 tctttatcat tctgattgct atttcctgcc gataagagat aaccagcaac tccttgtcgg
    12901 cgttgagtta attacccatt tctccagtga agatggcacg gtgcgtatcc cgacgagtcg
    12961 ggttatagcg caacttaccg aggagcaaca ttggcaatta ttttcagagc agttggaatt
    13021 actaaaatca tgccaacatt tttttattca acataaactc tttgcctggc ttaatttaac
    13081 gccacaagtg gctacattat tattagagag ggataattac gcaggcgaac tattaaaata
    13141 tccgtttatt gaactgttga ttaatgagaa ttacccgcat ctcaatgaag gaaaagataa
    13201 tcgcgatctt ttatccttat cgcaaatgta tccccttgtg ttaggcaatt tgggagcagg
    13261 taatagcaca atgaaagcgg tttttgatgg tcttttcacc cgcgtcatgc tggataaaag
    13321 ttttattcag caacaaatca cgcatcgctc cttcgaaccg tttattcgtg cgatccaggc
    13381 gcaaatttcc ccttgttgca attgcatcat cgcggggggc attgatacga cagaaatatt
    13441 ggcacagatt acccccttcg atttccatgc tctccaggga tgcctgtggc ctgccgttcc
    13501 tataaatcag ataacgacgt tggttcagcg ataaccctcc tgtttgctgg tgtttaagac
    13561 gagagtaacc gtctacacta tcaaacagga ggatctatga ccctgtcttt tattacccgc
    13621 tggcgcgatg aattgccaga aacctataca gcactttccc ctacgccttt aaataatgcc
    13681 cggctgattt ggcataatac cgaactcgct aacacgctga gtattccatc gtcgctgttt
    13741 aaaaatgctg caggtgtctg gggcggcgaa accctactgc ctggtatgtc accactggcg
    13801 caggtttaca gtggtcatca gttcggcgtc tgggcgggcc aactgggtga tgggcgcggc
    13861 attttacttg gcgaacaact gcttgctgat ggcactacaa tggactggca tctgaaaggt
    13921 gctggcctga cgccttattc gcgaatgggt gatggacggg cggttttacg ttcgacgata
    13981 cgagaaagtc tcgccagtga ggcgatgcat tatctgggca ttccgacgac ccgcgcgtta
    14041 agtatcgtca ccagcgattc gccagtgtat cgggaaacgg tggagccagg cgcgatgctg
    14101 atacgtgtgg caccaagtca tctgcgcttt ggtcatttcg aacattttta ctatcgccgc
    14161 gagccggaaa aggttcgtca gttagctgac tttgccattc gtcattactg gtcacatctt
    14221 gcagatgatg aggacaaata ccgtctctgg tttaccgatg ttgtcgcacg taccgcatcg
    14281 ttaattgccc aatggcagac ggtcggcttt gctcatgggg tgatgaatac cgacaacatg
    14341 tcgctgctgg ggctgacgct tgattacggg ccgtttggtt ttcttgatga ttacgaaccc
    14401 ggttttattt gtaatcactc ggatcatcaa gggcgttata gctttgataa tcaacctgct
    14461 gtcgcgttgt ggaatttaca gcgtctggcg cagacattgt caccatttgt tgccgtagat
    14521 gccctgaatg aggccctgga cagctatcag caggttttgt tgacgcatta tggacaacgg
    14581 atgcggcaga aactgggctt catgacggag caaaaagagg ataacgcgct actgaatgaa
    14641 ttattcagtc tgatggcgcg agagcgcagc gattataccc gcacattccg catgctgagt
    14701 ctgaccgagc agcacagcgc ggcgtcaccg ctacgtgatg agtttattga tcgtgcggca
    14761 tttgatgact ggtttgcccg ttatcggagg cgtttgcaac aagacgaggt tagcgatatt
    14821 gagcgtcagc aactgatgca aagcgttaac ccagctctgg tgttgcgcaa ctggttggcg
    14881 caacgggcga ttgaagcggc agaaaagggt gatatgacgg aattgcaccg cctgcatgag
    14941 gcgttgcgaa atcctttcag cgacagagat gatgactatg tcagtcgtcc acctgactgg
    15001 ggtaaacggc tggaagtcag ctgctcgagt taaaggctac ttagttaaca gcagcttgcc
    15061 agcctgagtt ttacggagca gatattcttg cccgtcatga tcgataatca gtttgccatc
    15121 cggacctaac agtgtctggc tggaaattcg ccgaataggc tgcggggaag cggtcttgtg
    15181 attggcatcc gtttcaggcg tgagttttct gctatccgta taacgcataa aaattaataa
    15241 tagttgatat aacaatgatt cttattatca atattatggc taaccagagc aagcagtaat
    15301 gtgctgagaa gacaacaagt tgcgcagaaa tgcccatccc atcaggaatg ggcacgcatt
    15361 cagaagcggg tatctaccgc agaggcgagt ttttcgacca ggcgttcggt atcttcccag
    15421 cccagacacg ggtcggtaat ggattgaccg taagtgagcg gctgactgcc gacgattttt
    15481 tgcgttcctt cgcgcaggaa actttccgcc ataattccag caatcgccgt agagccattg
    15541 cggatttgct gacaaatatc ctcacaaact tctaactggc gacggtgctg cttctggcag
    15601 ttaccgtggc tgaaatccac caccagatgt tcaggtaaat caaactcgtg cagcgtatcg
    15661 caggctgcgg cgatatcatc ggcatgataa ttcggttttt tgccgccacg cataataatg
    15721 tggccatacg ggttgccgct ggtctgatag atggtcatct gaccattttt gtctggcgag
    15781 aggaacatat ggctggcgcg ggctgcgcgg atagcatcca cggcaatccg cgtgttgcca
    15841 tcggtaccat ttttaaaacc taccggacag gagagtgccg aagccatttc acggtggatc
    15901 tgactttcgg tagtacgtgc gccaatcgcg ccccaactga ttaaatcagc aataaactga
    15961 ccggtcacca tatcgaggaa ctcagtcgcg gtagggacac ccagctcatt tacctgtaaa
    16021 agtaatttgc gcgccagctc cagaccgtga tttacccgat agctgccgtt taaatccgga
    16081 tcggagatta gccctttcca gccgacaaca gttcgtggtt tttcaaaata ggtgcgcatt
    16141 acgatttcca gccgtgactg gtactggttg cgcagcgact gcagacgggt ggcgtactcc
    16201 attgcagcgg tgagatcgtg gatcgagcag gggccaataa tgaccaacag tcgcttatct
    16261 tcaccattca gtattttttc aattctgcgg cgggagtcgg tgacatgggt ggcgacgcca
    16321 ggcgttacgg gataccgtag cgcgagttcg gcgggcgtta ccaggctctc aatacgcgca
    16381 gtacggagtt cgtcagttct gttcattaca agtctcagaa atttgttgct tctacggcag
    16441 gactaccggg aagtgatgcg catcacgata aaccaatcgc agctgatttc aagtgcgagg
    16501 tgtgtcacac ctcgccgggt ggttagcatg ataacaaaaa aataagctaa tgcactagtt
    16561 ctctagtaca ttcggcggct aagccccatg atatcgagga tcttggtggc aatctcttct
    16621 accgaataat tggtactgtt aatccacggg atctgatttt tacggtacaa cgcttctact
    16681 tccgcgactt ccatcctgca ctgacgaagc gaggcatagc ggctgttctc ccgacgttcc
    16741 tcgcgaatcg ccgccagacg ttccgggtcg atagtcaggc cgaacaattt atgctgaagc
    16801 ggtttgagcg acgcgggtag caccagattg tccatatcgt cggcaataaa ggggtagttt
    16861 gccgcgcgga taccaaactg cattgccaga tacagactgg tgggggtttt accacagcga
    16921 gaaacaccga gcaggatcac ctgagcctgg tccagattgc gcaacgaaat gccgtcatcg
    16981 tgggcgaggg tgtaatcaat cgccgcaatg cgcgcatcat atttattgag attattaggg
    17041 ttaaggccat gggtacgatg agcaatcggc gttggatcca gtttcatctc ttgttgtagc
    17101 ggggcaacca gcgcctgaac aatatcctgg caaaagcctt cactttgcaa gatgatggcg
    17161 cgaatctccg gcaacacgat ggagtagaag accagcgggc gcacacctgt ctggtgataa
    17221 attgcgtcaa tctgatcctt cactgcccgt gcacggctct cattttcgac aaacggcagc
    17281 gtaatgctgc tgatagtgac gggaaattgt gacattactg cgtgtcctaa tacctccgca
    17341 gttattgccg taccatcaga aatataaaaa acgtggcgat caacagcatt atccattttg
    17401 ttcttcccgt gatgcagaca taattctatg aaagcataaa ttaaaaacgc acagaagcgt
    17461 aggacgtaat gtctggttta taaaatgaac cttcaatttt attttttatg aaaacagcat
    17521 ttcattttta tggtttcgtt tataccgatg gtttatgtgg aaattgtcga atagagcagg
    17581 tttgcgcaac gctgggatca gtcttaaaaa gtaaaaaaat atatttgctt gaacgattca
    17641 ccgttttttt catccggtta aatatgcaaa gataaatgcg cagaaatgtg tttctcaaac
    17701 cgttcattta tcacaaaagg attgttcgat gtccaacaat ggctcgtcac cgctggtgct
    17761 ttggtataac caactcggca tgaatgatgt agacagggtt gggggcaaaa atgcctccct
    17821 gggtgaaatg attactaacc tttccggaat gggtgtttcc gttccgaatg gtttcgccac
    17881 aaccgccgac gcgtttaacc agtttctgga ccaaagcggc gtaaaccagc gcatttatga
    17941 actgctggat aaaacggata ttgacgatgt tacccagctt gcgaaagcgg gcgcgcaaat
    18001 ccgccagtgg attatcgaca ctcccttcca gcctgagctg gaaaatgcca tccgcgaagc
    18061 ttatgcacag ctttccgccg atgacgaaaa cgcctctttt gcggtgcgct cctccgctac
    18121 tgcagaagat atgccggacg cttcctttgc cggtcagcag gaaaccttcc tcaacgttca
    18181 gggttttgac gccgttctcg tggcagtgaa acatgtattt gcttctctgt ttaacgatcg
    18241 cgccatctct tatcgtgtgc accagggtta cgatcaccgt ggcgtggcgc tctccgccgg
    18301 tgttcaacgg atggtgcgct ctgacctcgc atcttctggc gtgatgttct ccattgatac
    18361 cgaatccggc tttgaccagg tggtgtttat cacttccgca tggggccttg gtgaaatggt
    18421 cgtgcagggc gcggttaacc cggatgagtt ttacgtccat aaaccgacac ttgcagcgaa
    18481 tcgcccggct atcgtgcgtc gcaccatggg gtcgaaaaaa atccgcatgg tttacgcgcc
    18541 gacccaggag cacggcaagc aggttaaaat cgaagacgta ccgcaggaac agcgtgacat
    18601 cttctcgctg accaacgaag aagtgcagga actggcaaaa caggccgtac aaattgagaa
    18661 acactacggt cgcccgatgg atattgagtg ggcgaaagat ggccacaccg gcaaactgtt
    18721 cattgtgcag gcgcgtccgg aaaccgtgcg ctcacgcggt caggtcatgg agcgttatac
    18781 gctgcattca cagggtaaga ttatcgccga aggccgtgct atcggtcatc gcatcggcgc
    18841 gggtccggtg aaagtcatcc atgacatcag cgaaatgaac cgcatcgaac ctggcgacgt
    18901 gctggtcact gacatgaccg acccggactg ggaaccgatc atgaagaaag catctgccat
    18961 cgtcaccaac cgtggcggtc gtacctgtca cgcggcgatc atcgctcgtg aactgggcat
    19021 tccggcggta gtgggctgtg gtgatgcaac agaacggatg aaagacggtg agaacgtcac
    19081 tgtttcttgt gccgaaggtg ataccggtta cgtctatgcg gagttgctgg aatttagcgt
    19141 gaaaagctcc agcgtagaaa cgatgccgga tctgccgttg aaagtgatga tgaacgtcgg
    19201 taacccggac cgtgctttcg acttcgcctg cctgccgaac gaaggcgtgg gccttgcgcg
    19261 tctggaattt atcatcaacc gtatgattgg cgtccaccca cgcgcactgc ttgagtttga
    19321 cgatcaggaa ccgcagttgc aaaacgaaat ccgcgagatg atgaaaggtt ttgattctcc
    19381 gcgtgaattt tacgttggtc gtctgactga agggatcgcg acgctgggtg ccgcgtttta
    19441 tccgaagcgc gtcattgtcc gtctctctga ttttaaatcg aacgaatatg ccaacctggt
    19501 cggtggtgag cgttacgagc cagatgaaga gaacccgatg ctcggcttcc gtggcgcggg
    19561 ccgctatgtt tccgacagct tccgcgactg cttcgcgctg gagtgtgaag cagtgaaacg
    19621 agtgcgcaac gacatggggc tgaccaacgt tgagatcatg atcccgttcg tgcgtaccgt
    19681 agatcaggcg aaagcggtgg ttgaagaact ggcgcgtcag gggctgaaac gtggcgagaa
    19741 cgggctgaaa atcatcatga tgtgtgaaat tccgtccaac gccttgctgg cagagcagtt
    19801 cctcgaatat ttcgacggct tctcaattgg ctcaaacgat atgacacagc tggcgctcgg
    19861 tctggatcgt gactccggcg tggtgtctga attgttcgat gagcgcaacg atgcggtgaa
    19921 agcattgctg tcgatggcta tccgtgccgc gaagaaacag ggcaaatatg tcgggatttg
    19981 cggtcagggt ccgtccgacc atgaagactt tgccgcatgg ttgatggaag aggggatcga
    20041 tagcctgtct ctgaacccgg acaccgtggt gcaaacctgg ttaagcctgg ctgaactgaa
    20101 gaaataaaat aaatccccgg cggcgttcag tcgccgggtt tatgtgatcc ccgaagataa
    20161 aacttattca atctcttcac agacatcctg cgttaatcgc cgcataatat cttttcttaa
    20221 caaaaacttt tgtattttac ctgaggcagt gcgcggtagt ttttcgatta ccacgatatg
    20281 ttcaggatat ttatattttg cgacccgttt acggctaaaa aaagccacca cctcttccag
    20341 cgataatgaa tgatgcggcg ctttcagcac gacataagcg catgatcgtt cacctaaacg
    20401 ttcatcgggc attgcaacca cacaggcatc gtgaatttta ggatgctgca ataaaatatc
    20461 ttccacttca cggctgctaa tattttcgcc gccgcggaca ataatatctt ttttgcgtcc
    20521 ggtaattttt atatagccag cctcatccat acggcagaga tcgccgctgt aataccagcc
    20581 ttcttcatcc agcgcacggg cggttaattc aggttcatca aaatatccca taaacacatt
    20641 ggggccacgc gaggcttctt caccttcgca acctggcggt aaggtcttgc gtgcgtcatc
    20701 gaccacttta atctctacac ctgcggcagc gtaaccatcg gtgtgcataa agcgcgacaa
    20761 aggatcatcg agattcacca ccgcatgcgg cgaactttct gtggaaccat aaacacttaa
    20821 taatttaatg ccgagctgct ggcattcacg cgccactttt ttggggattg tggttccgcc
    20881 gcaaagaaag aaacgcagca ctgacaggtc cgcgggttgt ttctctagta cattcaaaag
    20941 atcatagaca aacggcgttg cgcctagcat acaggtgcaa cgctgctgct caagcagcgc
    21001 gagacacgca tcaggagtga aaatatctaa caacacgctg cgagcgccaa ttaagaatgg
    21061 tgccgttacg ccatgcagaa agcccgttgc gtggccgagt ggcgcaggca tcataaagac
    21121 atcctgccag gtcagattca gccgcgcgca ataagcccgc tcactggcga gaatattgtt
    21181 atgcgttagc atcacgccct ttggcagacc ctcggttccg gaggtaaaca gcaccgcagc
    21241 taattcatcg ccgtgggtcg ttatcgccgt ggtcagtggg gtattgtcgg cgataatctg
    21301 acttaatgag agggaagagg tggcgggagc cagtttgtcc acgccgacaa tttgttgtag
    21361 ttgtggaagc tgattttgca gcggcaggat taaatctacc gggcgcgttt gtttaaacaa
    21421 cgtcggtgca aagaacattt ttgcctgaca cttattgagc acccacacca gttctgcttc
    21481 ccgccaggaa ggcaacagcg gcacggaaac cgcaccgatt ttcaggcagg caagatagat
    21541 aacggtaaat tcacaccagc caggcagttg aaatgcgatg cgatcgcctg attcaatacc
    21601 cttcgctaac atccagtttg ccagacagct cgcggcgtga tcgagcgcgc tataggtgta
    21661 cgatgcacca tgattatcga ccacggcaat tttgtctggc atcgcacgag cggtctgctg
    21721 ccagtaatcg gccagcgaag catcgcccca tagcccttgc tgacgatacg ccgcacgacg
    21781 ttgttcgttg aacgttaatg tcactttcat gttggactct cgaaacagaa catcaggccc
    21841 gagatgcggg cctgtgggat gcatgattag ccgtagcgaa aatcaatacc gaaggtgcct
    21901 gcgggatatt gccactgttc gagaatagtg ttaccgcaca gcacccgaca ggtgccgcac
    21961 tccagacaac cggcagaatc aaaatgaatg ttgccggcgt catcctgctt ataaagtccg
    22021 gcagggcagg ctttcattaa tttacggaat tcattgatat cgggatttgc cgccagaatg
    22081 atatgcgggt ggccctcatc aacatggaat ttattgacgc ctaatttgat gtcgacgtta
    22141 accgtagcgt tctggctcat agcgcggttg ctcccttaat gccatctttc agcaagttga
    22201 tcagcccaat tttcttcgca tgtcccatga tcattttgcg taccggctgg tttggtttgc
    22261 cgtcaatggt gaacatctcg ttcatgatgt cggcgaccat tcgtgggtat tggctaaaca
    22321 ggcgcgggtt ttccatcagc gccgggatct tgcggaaatg ctgcatatcg cgcatgacgc
    22381 agctttgttc cagctcacgt ttgtattgcg ccagactgct ggcggagaaa tcctcgcgtt
    22441 ctttggcggc gatcaccgtt gtggcggcag cctgagccga tgcaatggct aaatccatgc
    22501 cacgaactgt aaaacccaaa ttcaggcaga agcctgcggc gtcaccaacg atcatcacgc
    22561 catcgttaac catctgcggc accattgcca gaccgccttc tggcaccata tgcgcggaat
    22621 attcaagcag tttgccgccg ctaattagtg ggcgaatggc ggggtgttgt ttaaaatctt
    22681 ccagcatttt cggcacgctt ttttgcgcat gggcgatatc acccaatcca caaaccagcc
    22741 ccaaggatat ggaatccttg ttggtataga gaaatccccc gcccatcagg ccgtcagaag
    22801 gggaaccggc aaacagccag gcggcacctt cctcgcccgt aatattaaag cgatcgttga
    22861 tctgttctgg tgtgaggcca ataacctctt taacaccaac agcgtaatga tgcggatcgg
    22921 aagcgggaac cattcccagc gagcggccaa gcatcgagtt aacgccatca gccagaatca
    22981 ccacattcgc ttcgagaata tcatccccag cctgcacgcc agtgaccttg tttccttcac
    23041 gaaccagcgc atcgacgcga acgcctggga taaactgcgc gcccgcctgc tcggcttgtt
    23101 ccatcaacca cgggtccaga cgattacgca atacggtata agatgcgtgt tgcggaacat
    23161 ctggttgctc gcggtgaaaa tcgagggtaa cggcgctttc ttcggttaag aaggagattt
    23221 tctcgcgtgt gaccttgcgt tctaccggcg ctgatactgc aaagcctgga atgattgctt
    23281 caagtgtgtg ggcataaaga cgcccgccgg tcatgttttt acatccggca ctgtcgccgc
    23341 gttctatcac caggacatcc agcccggctc gcgccatgac cagtgcggca acgctaccag
    23401 caacgcccgc accgaccaca atggcatcaa atttgtcttc cgacatgcgt tatgctccct
    23461 tcccgctgcg tttacagcgg gagatgtggt tgaaagggga attagcggct caactggcta
    23521 atcagggcag ggacgacttt gtagatatcg cccaccagac cgtagtcggc ataattgaag
    23581 attggcgcat ttttatcttt attgatggcg acaatcactt ttgcgccgtt gccgccaacc
    23641 atatgctgga tctgcccgga gatccccagc gtcaggtaga gatcggattt cagcaacacg
    23701 ccggagacac cgatataacg ttcacgctcc atccagttct cgccttcggc aattggacgt
    23761 gaacagccga cttcagcatt cagcaccgcc gccagttcgt ggaccatttt caggtcatcc
    23821 tgcgccgcca gaccacgacc gacgccaacc acacgttttg ctttgctcag gtccacgctg
    23881 cttgcggctt tcgcacggcg ttcgcgacag agaatttcat gacgcggagc aacataaggt
    23941 accgtttctg tcgggcactg atgagaggta tcactggtgc acggttcctg aacaccgggt
    24001 gcaagggtaa tgatcgccag cgggctgttg atcttttcct gagcgaacgc taacccgcca
    24061 tacatccggt gttcggcgca aatgtgacca tcgacaatat ccaccgccgt ggcatcgttc
    24121 accagcgccg cattcagttg cacacttaac cgtgctgcca gagctttacc acgtttcgtc
    24181 gcggccaaca gcaacatagc ggggtgttta tctttcagca gggcagcaat gctttcggcg
    24241 taattttcag tgcgttgcag cgcgtcgttt tgctcaagaa cataaataca ttttggacca
    24301 taaggcataa ctgcctgcgc ctggtcggta ttttgtacaa tggcatacac ctgttggccc
    24361 cattgctgag cgccgccaaa cagttcagca taacgttcag gattatcgct aaagacccag
    24421 acgctgttta attgactcat cgtttcttct ccgtaggctt cagttcaggg ctttcttcag
    24481 atgttccgcc agctcggcaa tggcctccgg cgaatcgtta tcgataatga tgtgcttacg
    24541 ttctgtttgc ggcggtacgc gaatgccgac cagttcagca agtggcgcgc tctggctcca
    24601 gtcaatatca cttgcctgcc actgatttac cggtttttta cccgcgccga gaatggcttt
    24661 catcgaagga atgcgtggca cgttaatatc ggaggtgacg cagagcacgg ctggaacaga
    24721 gagttcaata acttcaacat catcttcaag cgtgcgttca atcaccagtg tattgccctg
    24781 acgctgaata gcactcactg cattaatcac cggaagttgc agaatttctc cgaccagcaa
    24841 gccaacctgc tgggcataaa ggtcgccgga accttcacca aagatcagta aatcgaagcc
    24901 gatcttttca actgctgccg ccagcgcttt tgcggtatcg agaggcagtg catgttcaag
    24961 ttgcgcatcc tgcaccaaat acaggctgtg cggcccgcgg gatagcacgt ctttgcgcac
    25021 tttcgagttc tgcaacaatg agccgccaac ggtcagcgcg gctatctcat cgtcatctgt
    25081 tgcaagctgg cttgcagctt caatggcatt gagatcgaac tggctgattt tggcgtcggc
    25141 attgtcgaaa ttcagggtgt attctggagt gacaacaatg tcctgttctt caggcaccag
    25201 cttaaagcag gttattattt tcatgatttc tcctgtgagt cgcatacgga accatctccg
    25261 atggaaggta aaaatggcct gaaaatagtg tgcgatagcg gtgcggggtt ttcgacggag
    25321 caagctgttt tccttgccaa caatcttgca aaaaaaatgc gatcgctaaa acaaaagtgc
    25381 catttgcggc aatatagtta ttccatgacc gtacaatctt gcattcagtg taaataatct
    25441 tgtttagctc tcgctaaacg cggttgtata accgcaatac acgccgtatt ttatccggca
    25501 tatattgttt ttgcttgctc atggaatagg gaggttgtca tgtatcaacg ctgttttgat
    25561 aatgccagtg aaacgctgtt tgtcgccggt aaaacgccac ggctttcacg ttttgcattt
    25621 agcgatgatc caaaatggga gtctggacat cacgttcatg acaatgaaac cgagctgatt
    25681 tacgtcaaga aaggggctgc aaggtttacc atcgattcat cgttatatgt cgcacatgcc
    25741 gatgatatag tggtgataga acgcggcagg ctgcatgcgg tggcctctga cgttaacgat
    25801 ccggcaacga cgtgtacctg tgcgctgtac ggctttcagt ttcagggggc tgaggaaaat
    25861 cagctactgc aaccgcattc ttgtccggta attgccgcgg ggcagggaaa agaagtcata
    25921 aaaaccttat ttaatgagct aagtgtgatt ttgccgcaaa gtaaaaatag ccaaacatct
    25981 tcgttatggg atgcatttgc ctatacgtta gccattcttt actacgaaaa ttttaaaaat
//
LOCUS       DAPGWD010000056        26005 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPGWD010000056 DAPGWD010000000
VERSION     DAPGWD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.056"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            377..1516
                     /gene="wza"
                     /locus_tag="RHX88_002242"
     CDS             377..1516
                     /gene="wza"
                     /locus_tag="RHX88_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDV2230930.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1522..1965
                     /gene="wzb"
                     /locus_tag="RHX88_002243"
     CDS             1522..1965
                     /gene="wzb"
                     /locus_tag="RHX88_002243"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDV2230931.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1968..4130
                     /gene="wzc"
                     /locus_tag="RHX88_002244"
     CDS             1968..4130
                     /gene="wzc"
                     /locus_tag="RHX88_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDV2230932.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4308..5147
                     /gene="wcaA"
                     /locus_tag="RHX88_002245"
     CDS             4308..5147
                     /gene="wcaA"
                     /locus_tag="RHX88_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDV2230933.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5150..5638
                     /gene="wcaB"
                     /locus_tag="RHX88_002246"
     CDS             5150..5638
                     /gene="wcaB"
                     /locus_tag="RHX88_002246"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDV2230934.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5635..6852
                     /gene="wcaC"
                     /locus_tag="RHX88_002247"
     CDS             5635..6852
                     /gene="wcaC"
                     /locus_tag="RHX88_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDV2230935.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6827..8044
                     /gene="wcaD"
                     /locus_tag="RHX88_002248"
     CDS             6827..8044
                     /gene="wcaD"
                     /locus_tag="RHX88_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDV2230936.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGVGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8055..8801
                     /gene="wcaE"
                     /locus_tag="RHX88_002249"
     CDS             8055..8801
                     /gene="wcaE"
                     /locus_tag="RHX88_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDV2230937.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNSIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8817..9365
                     /gene="wcaF"
                     /locus_tag="RHX88_002250"
     CDS             8817..9365
                     /gene="wcaF"
                     /locus_tag="RHX88_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDV2230938.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9391..10512
                     /gene="gmd"
                     /locus_tag="RHX88_002251"
     CDS             9391..10512
                     /gene="gmd"
                     /locus_tag="RHX88_002251"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDV2230939.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10515..11480
                     /gene="fcl"
                     /locus_tag="RHX88_002252"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10515..11480
                     /gene="fcl"
                     /locus_tag="RHX88_002252"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDV2230940.1"
                     /translation="MSKQRVFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11483..11962
                     /gene="gmm"
                     /locus_tag="RHX88_002253"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11483..11962
                     /gene="gmm"
                     /locus_tag="RHX88_002253"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDV2230941.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVSEEELLLPDEQHDDYRWLTPDALLASDNVHANSRSYFLAEKRAGVPGL"
     gene            11959..13182
                     /gene="wcaI"
                     /locus_tag="RHX88_002254"
     CDS             11959..13182
                     /gene="wcaI"
                     /locus_tag="RHX88_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDV2230942.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLVAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13185..14621
                     /gene="cpsB"
                     /locus_tag="RHX88_002255"
     CDS             13185..14621
                     /gene="cpsB"
                     /locus_tag="RHX88_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDV2230943.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLKGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     TRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14814..16184
                     /gene="cpsG"
                     /locus_tag="RHX88_002256"
                     /gene_synonym="manB"
     CDS             14814..16184
                     /gene="cpsG"
                     /locus_tag="RHX88_002256"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDV2230944.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPIEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16320..17714
                     /gene="wcaJ"
                     /locus_tag="RHX88_002257"
     CDS             16320..17714
                     /gene="wcaJ"
                     /locus_tag="RHX88_002257"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDV2230945.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     FYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17716..19194
                     /gene="wzxC"
                     /locus_tag="RHX88_002258"
     CDS             17716..19194
                     /gene="wzxC"
                     /locus_tag="RHX88_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDV2230946.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19470..20750
                     /gene="wcaK"
                     /locus_tag="RHX88_002259"
     CDS             19470..20750
                     /gene="wcaK"
                     /locus_tag="RHX88_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDV2230947.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKLLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERYTGMQMVQSVLERIGEVK"
     gene            20747..21967
                     /gene="wcaL"
                     /locus_tag="RHX88_002260"
     CDS             20747..21967
                     /gene="wcaL"
                     /locus_tag="RHX88_002260"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDV2230948.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGKLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21978..23372
                     /gene="wcaM"
                     /locus_tag="RHX88_002261"
     CDS             21978..23372
                     /gene="wcaM"
                     /locus_tag="RHX88_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDV2230949.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYTPHD
                     WIASFKQAFSEVQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     NHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGIFMAKEETLLSFANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23547..24440
                     /gene="galF"
                     /locus_tag="RHX88_002262"
     CDS             23547..24440
                     /gene="galF"
                     /locus_tag="RHX88_002262"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDV2230950.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            24812..25885
                     /gene="rfbB"
                     /locus_tag="RHX88_002263"
     CDS             24812..25885
                     /gene="rfbB"
                     /locus_tag="RHX88_002263"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDV2230951.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNSNEALPLFTEMTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVMTQGVVGETYNIGGRNEKKNLDVVNTICDL
                     LDEIVPKQVSYRDQIIYVTDRPGHDRRYAIDASKISDELGWKPQETFESGIRKTIGWY
                     LNNLEWCRRVQDGSYQRERLGLQ"
     gene            25886..>26005
                     /locus_tag="RHX88_002264"
     CDS             25886..>26005
                     /locus_tag="RHX88_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HDV2230952.1"
                     /translation="MKGIILAGGSGTRLYPVTMAVSKQLLPVYDKPMIYYPLST"
BASE COUNT         6404 a         6425 c         7028 g         6148 t
ORIGIN      
        1 ttgcctgcta ttccgttagc tgtaacactt cctcctgcat tattggagag ccaatattca
       61 attaccataa tgtcacctta tattcggaag ttaatttccg caataaccct ttctcatcaa
      121 cgattgtatg cagagagggt ttgaaaaatt aagctaaatc agtgtattgg tagctaaaaa
      181 gccaggggcg gtagcgtgtc tggatgcctg aaagaccagt ctgaattatt ctgtcaatag
      241 cctgcggatg aaatcaattt ttttaggact gatgccagtt aaatttgtat tcaattaact
      301 gcaaggtaaa tgtgcatggc gacattgcta ttaatagtgc acaggataat tactctgcca
      361 aagtgataaa taaacaatga tgaaatccaa aatgaaattg atgccattat tggtgtcagt
      421 aaccttgata agcggttgca cagtacttcc gggcagcaat atgtcgacga tgggcaaaga
      481 cgtcatcaaa cagcaggacg ctgatttcga tctcgacaaa atggtgaatg tttatccgtt
      541 gaccccgcgc ctgattgatc aattacgtcc acgacccaac gtagcgcgcc ccaatatgac
      601 gctggaaagt gagatcgcga attaccagta tcgcgtcggg ccgggggacg ttctgaatgt
      661 caccgtctgg gatcacccgg aactcaccac gccagccggt cagtaccgca gctccagtga
      721 caccggcaac tgggtacagc ctgacggcac tatgttttac ccgtatatcg gcaaggttca
      781 cgtagtcggg aaaacgctcg ctgaaatccg cagtgatatt accgggcgct tagcgacgta
      841 catcgctgac ccgcaggtgg acgttaatat cgccgccttc cgctcacaaa aggcctatat
      901 ctccggacag gtgaataaat ccggtcaaca ggcgatcacc aacgtaccac tgaccattct
      961 cgacgccatc aacgccgcag gtggcctgac cgacaccgct gactggcgca acgtggtgct
     1021 aacacacaat ggtcgtgaag aacgcatttc tttgcaggcg ctgatgcaaa acggcgacct
     1081 caatcagaac cgactgcttt atcccggcga tattctctac gtgccgcgta atgatgatct
     1141 gaaagtgttt gtgatgggtg aagtgaagaa acagagcacc ctgaaaatgg actttagcgg
     1201 catgaccctg actgaagccc tgggcaatgc cgaaggtatc gacatgacca cctccaacgc
     1261 cagcggcatc tttgtcattc gtccgctgaa aggcgagggc gggcgtaatg gcaagattgc
     1321 caatatctac cagctggata tgtccgatgc gacgtcgctg gtgatggcga cagaattccg
     1381 cctgcaacct tatgacgtgg tgtatgtcac caccgccccg gtttcccgct ggaaccgtct
     1441 gatcaatcag ttgctgccaa ctattagcgg tgtccgttac atgacggata cagccagcga
     1501 cattcataac tggtaatcgt catgtttaac aacatcttag ttgtctgtgt cggcaatatt
     1561 tgccgttccc cgacggcgga acgcttactg caacgttatc acccggagct gaaagtggag
     1621 tccgctggac tcggcgcgct ggtcggtaag ggcgctgatc ctaccgctat cagcgtcgcc
     1681 gcagaacatc aactgtctct ggaaggtcac tgtgcacggc aaatcagccg ccgcctgtgt
     1741 cgcaactatg acctgatttt gaccatggaa aagcgccata tcgaacgctt atgcgagatg
     1801 gcacctgaga tgcgcggcaa agtgatgctg tttggtcact gggataacga atgtgaaatc
     1861 cccgatccgt atcgcaaaag ccgggaaacg tttgcagcgg tgtacacatt acttgaacgg
     1921 tctgcccgcc agtgggcgca ggcattgaac gcagagcagg tataagaatg acagaaaaag
     1981 taaaacaaca tgccgctccg gtaacgggca gtgatgaaat cgatattggt cgcctggtcg
     2041 gcaccgtcat tgaagcgcgc tggtgggtga ttggcatcac cgctgtattt gccctctgtg
     2101 ctgtggttta cactttcttc gccacgccga tttatagtgc cgacgcactg gtacaaatcg
     2161 aacaaaacag cggcaattcg ttagtgcagg acattggctc ggcattagcc aacaaaccgc
     2221 ctgcatcgga cgccgagatc cagttgattc gttcgcgtct ggtgcttggt aaaacggtgg
     2281 acgatctaga cctcgatatt gcggtgagca aaaacacgtt cccgattttc ggtgcgggct
     2341 gggatcgttt gatgggacgt cagaacgaga cggtgaaagt gaccaccttt aaccgcccga
     2401 aagagatggc ggaccaggtg tttacgctta atgtgctgga tgacaaaaac tacactctga
     2461 gcagtgatgg cggctttagc gcccgtgggc aagcgggcca gatgctgaaa aaagaaggcg
     2521 tcacgctgat ggttgaagcc attcacgccc gcccgggcag tgagtttacc gtcaccaaat
     2581 actccacgtt ggggatgatc aaccaactgc aaaacagcct gacggtaacg gagaacggca
     2641 aagacgcagg cgtactgagc ctgacttata ccggtgaaga tcgcgaacag atccgcgaca
     2701 ttcttaacag catcgcccgt aactatcagg aacaaaatat tgagcgcaaa tcggcggaag
     2761 cgtcgaaaag cctcgctttc ctcgcgcaac agttaccgga agtacgtagc cgcctcgatg
     2821 ttgccgaaaa caaactgaat gccttccgtc aggataaaga ttctgttgat ctgccgctgg
     2881 aagcgaaagc ggtgctcgat tcgatggtga acatcgacgc gcagttgaac gaactgacct
     2941 ttaaagaggc ggaaatctcc aagctgtaca ccaaagttca ccccgcgtac cgcacgctgc
     3001 tggagaaacg tcaggcgctg gaagacgaaa aagccaaact taatggtcgc gtaacggcga
     3061 tgccgaaaac ccagcaggaa attgtccgtc tgacccgcga tgtcgagtct ggtcagcagg
     3121 tctatatgca actgctgaat aaagagcagg agctgaaaat caccgaagcc agcaccgtcg
     3181 gcgatgtgcg cattgttgac ccggcaatca cccagccagg tgtactgaaa ccgaagaaag
     3241 ggctgattat ccttggggcg attattcttg gcctgatgct ctctatcgtg ggtgtgctgc
     3301 tgcgctcgtt gtttaatcgc ggcatcgaaa gcccgcaggt gctggaagaa cacggtatca
     3361 gcgtctatgc cagcatcccg ttgtcggaat ggcagaaagc gcgcgatagc gtcaaaacca
     3421 tcaaagggat taaacgctat aaacagagcc agctactggc ggtggggaat ccaaccgatc
     3481 tggcgattga agccatccgc agtctgcgta ccagtctgca cttcgcgatg atgcaggcgc
     3541 agaacaatgt gttgatgatg accggggtta gcccgtcaat cggtaaaacc tttgtctgcg
     3601 ccaacctggc ggcggtgatc agccagacca ataaacgcgt gttgttgatc gactgcgata
     3661 tgcgcaaagg ctacacccac gagctgttgg gcaccaataa cgtcaatggc ctgtcggaaa
     3721 ttctgattgg tcagggcgat attactaccg cagctaaacc gacctctatt gccaaatttg
     3781 acctgatccc gcgcggtcag gtgccgccaa atccttctga actgttgatg agcgaacgct
     3841 ttgccgaact ggtgaactgg gcgagtaaaa actatgacct ggtgttgatt gatacgccgc
     3901 cgattctggc agtgaccgat gcggcaattg ttggtcgtca tgtcggaacc acattaatgg
     3961 tggcgcgtta tgcggtcaac acattgaaag aagtggaaac cagtctgagc cgctttgagc
     4021 aaaacggtat tccggtgaaa ggggtgattc tgaactccat cttccgccgc gccagcgcgt
     4081 atcaggatta tggctattac gaatacgaat ataagtcgga tgcgaaataa cgacatccgc
     4141 tgtcggatgc gatgctgacg catcttatcc gacctacctt cacgccgcat ccggcaagca
     4201 aaccagctcc gtaggccgga taaggcgctc acgccgcatc cggcaacaga acccgtaaac
     4261 aagcttataa accggcagaa catctcgata ctccgggaga acaacctatg aaagacaatc
     4321 cgctgatctc aatctatatg ccgacctgga accgccaaca actggcgatt cgggcgataa
     4381 aatcggtcct gcgccaggac tacagcaact gggagatgat catcgtggat gattgctcca
     4441 cctcctggga acaactgcaa cagtacgtca ccgccctcaa cgatccgcgt attacctaca
     4501 ttcacaacga cattaacagc ggggcgtgcg cggtacgtaa ccaggcgatt atgctggcgc
     4561 aaggggaata cattaccggg atcgatgacg atgatgaatg gacgcccaac cgcctgagcg
     4621 tcttcctcgc ccataaacag caactggtca ctcacgcctt tttgtacgct aacgactacg
     4681 tatgccaggg agaagtttat tcccaaccgg caagcttacc actgtatccc aaatcgccgt
     4741 actcacgccg cctgttctat aaacgcaata tcattggcaa tcaggttttt acctgggcgt
     4801 ggcgtttcaa agagtgcttg ttcgataccg aactgaaagc agcacaggat tacgacatct
     4861 tcctgcggat ggtggtggag tatggcgaac cgtggaaagt ggaagaggcg acgcagatcc
     4921 tgcatatcaa tcacggtgaa atgcagatca cctcgtcacc gaaaaaattc tccgggtact
     4981 tccactttta ccgcaagcac aaagataaat ttgaccgtgc aagcaaaaaa tatcagctgt
     5041 ttaccctcta ccagatccgc aataaacgta tgacctggcg cactttgctg acgctgcttt
     5101 cggtgcgtaa cggcaaacgt ctggctgatg gtattcgggg gcgctaaaga tgctggaaga
     5161 tctgcgcgcc aacagctgga gtttacgtcc gtgctgcatg gttctagcct atcgcgtcgc
     5221 tcatttttgt tcggtgtggc gcaaaaagaa cgtcctcaac aatctgtggg cggccccgct
     5281 gctggtgctg tatcgcatta tcaccgaatg ctttttcggt tatgaaatcc aggccgccgc
     5341 gaccattggc cgccgcttta ctatccatca cggttacgcc gtagtgatca ataaaaacgt
     5401 ggtagcgggg gatgatttca ccattcgcca cggcgtcact atcggcaatc gtggtgccga
     5461 taacatggca tgtccacata ttggcaacgg cgtcgaactc ggtgccaacg ttattattct
     5521 tggtgatatc acgcttggta acaacgtcac cgtgggtgcg ggcagcgtgg tgctcgactc
     5581 tgtcccggac aacgcgctgg tggtgggaga aaaagcgcga gtgaaggtaa ttaaatgaat
     5641 attttgcaat ttaatgtgcg actggcggaa ggcggggcag caggtgtggc gttagatctc
     5701 caccagcgtg cgctgcaaca ggggctagcg tcacattttg tctacggtta cggcaaaggc
     5761 ggcaaagaga gcgtcagcca tcaaaactat ccgcaggtca tcaaacatac gccgcggatg
     5821 actgcaatgg cgaatattgt cctgtttcgt ctgtttaatc gcgatctgtt tggcaatttc
     5881 aatgagttat atcgcaccat tactcgcaca ccgggtccgg tggtcctgca ttttcatgtg
     5941 ctgcacagct actggctaaa tcttaagagc gtggtgcgct tttgcgaaaa agtgaaaaac
     6001 cataaaccgg acgtcactct ggtctggacg ctgcacgacc actggagtgt taccggacgc
     6061 tgcgccttta ccgacggttg cgaaggctgg aaaacaggct gccagaaatg cccgacctta
     6121 aataattatc cgccggtgaa gattgatcgg gcgcaccagc tggtggcggg caaacgccag
     6181 ttattccgtg agatgctggc gctgggctgt cagtttattt cccccagcca gcatgtggct
     6241 gacgctttca atagcctgta cggtccaggg cgttgccgga ttatcaataa cggtattgat
     6301 atggcaaccg aagcgattct ggcggatctg cctccggtgc gcgaaaccca gggtaagccg
     6361 aaaatcgcgg tggtggcgca tgacctgcgt tacgacggca agactaacca gcaactggtg
     6421 cgtgagatga tggcgctggg cgacaaaatc gaactgcata cctttggtaa gttctcgccg
     6481 ttcaccgctg gtaacgtggt taatcacggc tttgaaaccg acaaacgtaa gctgatgagc
     6541 gcgctcaatc agatggatgc gctggtattc agttctcgcg tcgataacta cccgctgatt
     6601 ttgtgtgagg cgctatcgat tggcgtgccg gtgattgcca cccatagcga cgcggcgcgg
     6661 gaagtgctgc aaaaatccgg cggtaaaacc gtcagcgaag aagacgtgct gcaactggtg
     6721 cagttaagca aaccggaaat cgcgcaggcg atatttggta ccacgttggc cgggttcagc
     6781 caacgtagcc gcgccgccta cagtggacaa cagatgctgg aggagtatgt caacttctat
     6841 cagaatctgt agctacctgc tgctgccgct gatctacctg ctggttaacg tcaaaatcgc
     6901 ccagcttggt gaaagtttcc ccatcactat cgtcactttt ttacctgtct tgttactgct
     6961 gtttttagaa cgcatcagcg ttaaaaaatt gatgattgcc ttaggggttg gcgcggggct
     7021 cacggcgttc aactatctgt ttggtcagtc gttggatgcc agtaaatacg tcacttcaac
     7081 catgctgttt gtctatattg tgatcattat tggcatggtg tggagtattc gctttaaaac
     7141 gatttcgcca cacaaccatc gcaagatatt acgtttcttt tatctggtgg tcgggctggt
     7201 ggtggcgctg gcggcggtgg agatggcgca aattatcctc accggtggca gcagtattat
     7261 ggagtcgatt tcgaaatatc tgatttatag caacagctat gtactgaatt tcattaaatt
     7321 cggcggcaag cgcacgacag cactttattt cgaaccggca tttttcgctc tggcattaat
     7381 ctcaatttgg ctcagcatca aacagtttgg tatcaaaacg cctaaaacag atgctatgat
     7441 tctcgcaggg ataatattat ccggatcgtt ttcaggggtt atgaccttta tcctgtttta
     7501 tttgctggag tgggcattcc aatatctgaa taaagaggcg attaagaaaa agttaccgtt
     7561 agcattgatt tctctggctg tattcctggt tggtgtggta atcgcgtttc cttatatttc
     7621 cacccgtctg ggcgatttag gtacggaagg atcgtcatcg tattatcgta ttgtcggtcc
     7681 gctggtgatg gtcggttatt ctttgaccca tattgacggt gtagtcagat ttggctcact
     7741 ttatgaatat gtcgcatcat tcggaatatt taacggtgcg gatgtcggaa aaaccataga
     7801 caatggtttg tatctgctga ttatttattt ttcctggttc gcggtgtttt tatcgctgtg
     7861 gtacatgggg aaagtgataa aaatgatgat caacgctttt ggtgataacc gcaattttcg
     7921 cgtgcaatta tatcttttta ctccggtttc gctgtttttt accggttcga tatttagccc
     7981 ggaatatgca tttttaatcg tctgtccgtt tattttgcga aaagcgttaa atattacgag
     8041 gtaagaataa gaacatgttg cttagcataa tcactgtcgc gtttcgtaac ctcgaaggga
     8101 tagtcaaaac acatgcctcg ctggcgcatc tggcgcaggc ggacgatatc agcttcgaat
     8161 ggattgttgt cgatggcggt tccaacgacg gtacccgtga gtatctggag aatctcaata
     8221 gtatctataa cctacgcttt gtcagcgagc cagataacgg tatctacgac gccatgaaca
     8281 agggcattgc gatggcacaa ggcaagttcg cgttgtttct caactcgggc gatatttttc
     8341 atcaggatgc cgcaaatttt gttcgtaagt taaaaatgca aaaagataac gtgatgatca
     8401 ccggcgatgc gctgctggat tttggcgacg ggcataaaat taagcgtagt gctaaaccgg
     8461 gttggtatat ttatcacagc ctgcccgcca gccatcaggc gatatttttc ccggtatccg
     8521 gtttgaaaaa atggcgttat gacctggaat ataaagtttc ttctgattac gcgctggcag
     8581 ctaaaatgta taaagcaggt tatgcattta aaaaactcaa tggcctggtg tctgaatttt
     8641 ccatgggtgg ggtatcaacc accaataata tggaattgtg tgctgacgcc aaaaaagtcc
     8701 aacggcagat attacatgtg cctggcttct gggcagaatt atcctggcat ttacgccaac
     8761 gtactacctc aaagacgaaa gccttatata acaaaagctg aatataaggg aaaacgatgc
     8821 aggatttaag cggtttctcg gtgccgaaag ggttccgggg cggcaacgct attaaagtgc
     8881 aattatggtg ggcagtacag gcaacaatat ttgcctggtc gccacaagta ttgtatcgct
     8941 ggcgggcttt tttattacgt ttattcggag caaaaatagg aaaaaacgta gttattcgtc
     9001 cgtcagtaaa aattacctat ccgtggaaat taaccttagg tgattacgcg tgggtcggcg
     9061 atgacgtcaa tttatatacc ctcggtgaaa taaccattgg cgcacattcg gttatatcgc
     9121 aaaaaagtta tttatgcacc ggtagccacg accatgcaag tcaacatttc accattaacg
     9181 ccacgcctat tgtgattggc gagaaatgct ggctggcaac cgatgtcttt gttgccccag
     9241 gcgtcacaat cggcgacggc actgtcgtgg gtgcacgaag cagtgttttt aaatcgcttc
     9301 cggcaaatgt ggtttgccgg gggaatcccg cagtggtgat acgcgaacgc gttgaaactg
     9361 aataaattca aaaatacaga ggaataagac atgtcaaaag tcgctctcat caccggtgta
     9421 accggacaag acggttctta cctggcagag tttctgctgg aaaaaggtta cgaggtgcat
     9481 ggtattaagc gtcgcgcatc gtcattcaac accgagcgcg tggatcacat ttatcaggat
     9541 ccgcacacct gcaacccgaa attccatctg cattatggcg acctgagtga tacctctaac
     9601 ctgacgcgca ttttgcgtga agtacagccg gatgaagtgt acaacctggg ggcaatgagc
     9661 cacgttgcgg tctcttttga gtcaccagaa tataccgctg acgtcgacgc gatgggtacg
     9721 ctgcgcctgc tggaggcgat ccgcttcctc ggtctggaaa agaaaactcg tttctatcag
     9781 gcttccacct ctgaactgta tggtctggtg caggaaattc cgcagaaaga gaccacgccg
     9841 ttctacccgc gatctccgta tgcggtcgcc aaactgtacg cctactggat caccgttaac
     9901 taccgtgaat cctacggcat gtacgcctgt aacggaattc tcttcaacca tgaatccccg
     9961 cgccgcggcg aaaccttcgt tacccgcaaa atcacccgcg caatcgccaa catcgcccag
    10021 gggctggagt cgtgcctgta cctcggcaat atggattccc tgcgtgactg gggccacgcc
    10081 aaagactacg taaaaatgca gtggatgatg ctgcagcagg aacagccgga agatttcgtt
    10141 atcgcgaccg gcgttcagta ctccgtgcgt cagttcgtgg aaatggcggc agcacagctg
    10201 ggcatcaaac tgcgctttga aggcacgggc gttgaagaga agggcattgt ggtttccgtc
    10261 accgggcatg acgcgccggg cgttaaaccg ggtgatgtga ttatcgctgt tgacccgcgt
    10321 tacttccgtc cggctgaagt tgaaacgctg ctcggcgacc cgaccaaagc gcacgaaaaa
    10381 ctgggctgga aaccggaaat caccctcaga gagatggtgt ctgaaatggt ggctaatgac
    10441 ctcgaagcgg cgaaaaaaca ctctctgctg aaatctcacg gctacgacgt ggcgatcgcg
    10501 ctggagtcat aagcatgagt aaacaacgag tttttattgc tggtcatcgc gggatggtcg
    10561 gttccgccat caggcggcag ctcgaacagc gcggtgatgt ggaactggta ttacgcaccc
    10621 gcgacgagct gaacctgctg gacagccgcg ccgtgcatga tttctttgcc agcgaacgta
    10681 ttgaccaggt ctatctggcg gcggcgaaag ttggcggcat tgttgccaac aacacctatc
    10741 cggcggattt catctaccag aacatgatga ttgagagcaa catcattcac gccgcgcatc
    10801 agaacgacgt gaacaaactg ctgtttctcg gatcgtcctg catctacccg aaactggcaa
    10861 aacagccgat ggcagaaagc gagttgttgc agggcacgct ggagccgact aacgagcctt
    10921 atgctattgc caaaatcgcc gggatcaaac tgtgcgaatc atacaaccgc cagtacggac
    10981 gcgattaccg ctcagtcatg ccgaccaacc tgtacgggcc acacgacaac ttccacccga
    11041 gtaattcgca tgtgatccca gcattgctgc gtcgcttcca cgaggcgacg gcacagaatg
    11101 cgccggacgt ggtggtatgg ggcagcggta caccgatgcg cgaatttctg cacgtcgatg
    11161 atatggcggc ggcgagcatt catgtcatgg agctggcgca tgaagtctgg ctggagaaca
    11221 cccagccgat gttgtcgcac attaacgtcg gcacgggcgt tgactgcact atccgcgagc
    11281 tggcgcaaac catcgccaaa gtggtgggtt acaaaggccg ggtggttttt gatgccagca
    11341 aaccggatgg cacgccgcgc aaactgctgg atgtgacgcg cctgcatcag cttggctggt
    11401 atcacgaaat ctcactggaa gcggggcttg ccagcactta ccagtggttc cttgagaatc
    11461 aagaccgctt tcgggggtaa tgatgttttt acgtcaggaa gactttgcca cggtagtgcg
    11521 ctccactccg cttgtctctc tcgactttat tgtcgagaac agtcgcggcg agtttctgct
    11581 tggcaaaaga accaaccgcc cggcgcaggg ttactggttt gtgccgggag ggcgcgtgca
    11641 gaaagacgaa acgctggaag ccgcatttga gcggctgacg atggcggaac tggggctgcg
    11701 tttgccgata acagcaggcc agttttacgg tgtctggcag cacttttatg acgataactt
    11761 ctctggcacg gatttcacca ctcactatgt ggtgctcggt tttcgcttca gagtatcgga
    11821 agaagagctg ttactgccgg atgagcagca tgacgattac cgctggctga cgccggatgc
    11881 actgctcgcc agtgacaatg ttcacgctaa cagccgctcc tattttctcg ctgagaagcg
    11941 tgccggagta cccggattat gaaaatactg gtctacggca ttaactactc gccggagtta
    12001 accggcatcg gcaaatacac tggcgagatg gtggaatggc tggtagcaca aggtcatgag
    12061 gtgcgggtca ttaccgcacc gccttactac ccgcagtggc aggtgggcga gaactattcc
    12121 gcctggcgct acaaacgaga agagggggcc gccacggtgt ggcgctgccc gctgtacgtg
    12181 ccaaaacagc cgagcaccct gaaacgcctg ttacatctcg gcagttttgc cgtcagcagt
    12241 ttctttcctc tgatggcgca acgtcgctgg aagccggatc gcattatcgg cgtggtgcca
    12301 acgctgtttt gcacgccggg aatgcgcctg ctggcgaaac tctctggtgc gcgtaccgtg
    12361 ctgcatattc aggattacga agtggacgcc atgctggggc tgggccttgc cggaaaaggc
    12421 aaaggcggca aagtggcaca gctggcaacg gcgttcgaac gtagcggact gcataacgtc
    12481 gataacgttt ccacgatttc gcgttcgatg atgaataaag ccatcgaaaa aggcgtggcg
    12541 gcggataacg tcatcttctt ccccaactgg tcggaaattg cccgttttca acatgttgca
    12601 gacgccgatg ttgatgccct tcgtaaccag cttggcctgc cggataacaa aaaaatcatt
    12661 ctttactccg gcaatattgg tgaaaagcag gggctggaaa acgttattga agctgccgat
    12721 cgtctgcgcg atgaaccgct gatttttgcc attgtcgggc agggcggcgg caaagcgcgg
    12781 ctggaaaaaa tggcgcagca gcgtggactg cgcaacatgc aatttttccc gctgcaatcg
    12841 tatgacgctt tacccgcact gctgaagatg ggcgattgcc atctggtggt gcaaaaacgc
    12901 ggcgcggcag atgccgtatt gccgtcgaaa ctgaccaata ttctggcggt aggcggtaac
    12961 gcggtgatta ctgcagaagc ccacacagaa ctgggacagc tttgcgaaac ctttccgggc
    13021 attgcggttt gcgttgaacc ggaatcggtc gaggcgctgg tggcggggat tcgtcaggcg
    13081 ctcctgctgc ccaaacacaa cacggtggca cgtgaatatg ccgaacgcac gctcgataaa
    13141 gagaacgtgt tacgtcaatt tataaatgat attcggggat aattatggcg cagtcgaaac
    13201 tctatccagt tgtgatggca ggtggctccg gtagccgctt atggccgctt tcccgcgtac
    13261 tttaccccaa gcagttttta tgcctgaaag gcgatctcac catgctgcaa accaccatct
    13321 gccgcctgaa aggcgtagag tgcgaaagcc cggtggtgat ttgcaatgag cagcaccgct
    13381 ttattgtcgc ggaacagctg cgccaactga acaaactcac cgagaacatt attctcgaac
    13441 cggcagggcg taacactgca ccagccattg cgctggcggc gctggcggca acacgtcata
    13501 gtccggagag cgacccgtta atgctggtgc tggcggcgga tcatgtgatt gccgatgaag
    13561 acgcgttccg tgccgccgtg cgtaatgcca tgccgtatgc cgaagcgggc aagctggtga
    13621 cctttggcat tgtgccggat ctaccagaaa ccggttatgg ctatattcgt cgcggtgaag
    13681 tatctgcggg cgagcaggat acggtggcct ttgaagtggc gcagtttgtc gaaaaaccga
    13741 atctggaaac cgcccaggcc tatgtagcaa gtggcgaata ttactggaac agcggtatgt
    13801 tcctgttccg tgccggacgc tatctcgaag aactgaaaaa atatcgtccg gatattctcg
    13861 atgcctgcga aaaagcgatg agcgccgtcg atccggatct cgattttatt cgcgtggatg
    13921 aagaagcgtt tctcgcctgc ccggaagagt cggtggatta cgcggtcatg gaacgtacgg
    13981 cagatgccgt tgtggtgccg atggatgcgg gctggagcga tgtcggttcc tggtcctctt
    14041 tatgggagat cagcgcccac accgcggaag gcaacgtttg ccacggcgat gtgattaatc
    14101 acaaaactga aaacagctat gtgtacgccg aatctggcct ggtcaccacc gtcggggtga
    14161 aagatctggt ggtggtgcag accaaagatg cggtgctgat tgccgaccgt aacgcggtgc
    14221 aggatgtgaa aaaagtggtc gagcagatca aagccgatgg tcgccatgag catcgggtgc
    14281 atcgcgaagt ctatcgcccg tggggcaaat atgactctat cgacgcgggc gaccgctacc
    14341 aggtgaaacg catcaccgtg aaaccgggcg aaggcttgtc ggtacagatg caccatcacc
    14401 gcgcggaaca ctgggtggtg gtcgcgggaa cggcaaaagt cactatcgac ggtgatatca
    14461 aactgcttgg tgaaaacgag tccatttata ttccgctggg ggcgacgcat tgcctggaaa
    14521 acccggggaa aattccgctc gatttaattg aagtgcgctc cggctcttat ctcgaagagg
    14581 atgatgtggt gcgcttcgcg gatcgctacg gacgggtgta aacgtcgcat caggcgctgt
    14641 ttgtcggatg cggcgtgaac gccttatcca acctacggtt cggatttgta ggcctgataa
    14701 gacgcggcag cgtcgcatca ggcaatgaat gcgaaaccga gatgtaaata acgacaaaaa
    14761 caaaattggc cgtttcggtc agggccaact attgcctgaa aaagggtaac gacatgaaaa
    14821 aattaacctg ctttaaagcc tatgatattc gcgggaaatt aggcgaagaa ctgaatgaag
    14881 atatcgcctg gcgcattggt cgcgcctatg gcgaatttct caaaccgaaa accattgtgt
    14941 taggcggtga tgtccgtctc accagcgaaa ccttaaaact ggcgctggca aaaggtttac
    15001 aggatgcggg cgttgacgtg ttggatattg gtatgtccgg caccgaagag atctatttcg
    15061 ccacgttcca tctcggcgtg gatggcggca ttgaagttac cgccagccat aatccgatgg
    15121 attataacgg catgaaactg gtgcgcgaag gggctcgccc aatcagcggt gataccggac
    15181 tgcgcgacgt ccagcgtctg gcagaagcca acgactttcc tcccgtcgat gaaaccaaac
    15241 gcggtcgcta tcagcaaatc aacctgcgtg acgcttacgt tgatcacctg ttcggttata
    15301 tcaatgtcaa aaacctcacg ccgctcaagc tggtgattaa ctccgggaat ggcgcagcgg
    15361 gtccggtggt ggacgccatt gaagcccgct ttaaagccct cggcgcaccc gtggaattaa
    15421 tcaaagtgca caacacgccg gacggcaatt tccccaacgg tattcctaac ccgctactgc
    15481 cggaatgccg cgacgacacc cgcaatgcgg tcatcaaaca cggcgcggat atgggcattg
    15541 cctttgatgg cgattttgac cgctgtttcc tgtttgacga aaaagggcag tttatcgagg
    15601 gctactacat tgtcggcctg ttggcagaag cattcctcga aaaaaatccc ggcgcgaaga
    15661 tcatccacga tccacgtctc tcctggaata ccgttgatgt ggtgactgcc gcaggcggca
    15721 ctccggtgat gtcgaaaacc ggacacgcct ttattaaaga acggatgcgc aaggaagacg
    15781 ccatctacgg tggcgaaatg agcgcccacc attacttccg tgatttcgct tactgcgaca
    15841 gcggcatgat cccgtggctg ctggtcgccg aactggtgtg tctgaaagga aaaacgctgg
    15901 gcgaactggt gcgcgatcgg atggcggcgt ttccggcaag cggtgagatc aacagcaaac
    15961 tggcgcaccc cattgaggcg attaaccgcg tcgaacagca ttttagccgt gaggcgctgg
    16021 cggtggatcg caccgatggc atcagcatga cctttgccga ctggcgcttt aacctgcgca
    16081 cctccaacac cgaaccggtg gtgcggttga atgtggaatc gcgcggtgat gtaccgctga
    16141 tggaagcgcg aacgcgaact ctgctgacgt tgctgaacga gtaatgtcat tactttcccc
    16201 ctcaccctga cgctctactg cctgcggaga gaagtgagca cggaactttc ttctccccct
    16261 cgcccctttg gggagagggc cggggtgagg ggcgaggcga caatagaaca ggaacaacga
    16321 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
    16381 aacgcttttc agatatcacc atcatgtttg ccggactatg gctggtttgc gaagtgagcg
    16441 gactgtcatt cttctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
    16501 tgctgggtgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
    16561 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
    16621 tcaataatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagta
    16681 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
    16741 gctataacaa gcgcatggtc gcggtggcgg gggatttagc cgccggacaa atgctgatgg
    16801 agagcttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
    16861 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcga
    16921 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcgcgagtga
    16981 aaaaactggt ccatcaactg gcggacacca cctgttcggt gctgctgatc cccgacgtct
    17041 ttaccttcaa cattctccat tcacgcctcg aagagatgaa cggcgtaccg gtggtgccgc
    17101 tttacgacac gccgctttcc ggggttaacc gcctgctcaa acgtgcggaa gacattgtgc
    17161 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
    17221 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
    17281 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgactc
    17341 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
    17401 tggatgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcgggccac
    17461 gtccacacgc agtagcgcat aacgaacagt atcgacagct cattgaaggc tacatgctgc
    17521 gccacaaagt gaaaccgggc attaccggat gggcgcagat taacggctgg cgcggcgaaa
    17581 ccgacacact ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
    17641 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaaaggc ttcgttaaca
    17701 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggcg
    17761 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
    17821 atagataacc accagttcgg tttgcttacc gtgtcgctgg tgatcattgc gctggccgat
    17881 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagccacctt
    17941 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtgtg tgtggcggtg
    18001 tttttgttga gtgatgccat tggtgacgtg ctgaataacc cggacctggc accgttgatt
    18061 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc agcagttccg cgcgttgatg
    18121 caaaaggaac tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
    18181 ttcactttta cggtggttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
    18241 tatctggtca atagtgcggt aagaacgctg ctgtttggct actttggccg caaaatttat
    18301 cgccccggtc tgcacttctc gctggcgtcg gtggcaccga acttacgctt tggtgcctgg
    18361 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
    18421 cgtattctcg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgttgtg
    18481 ccaccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
    18541 caggacgaca ccgaaaagct gcgcgttaac ttctacaagc tgctgtcggt agtggggatt
    18601 atcaactttc cggcgctgct cgggctgatg gtggtgtcga ataattttgt accgctggtc
    18661 tttggtgaga agtggagcag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
    18721 ctgcgctcag tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    18781 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    18841 cagatggcgg gcgcaatcgg cgtcacgctt ggcttcctgc tggtgcagat tatcaacacc
    18901 attctgagtt acttcgtgat gattaaaccg gtactcggtt ccagttatcg ccagtacatc
    18961 ctgagtttgt ggctgccgtt ttacctctcg ctgccgacgc tggtggtcag ttatgcgctg
    19021 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    19081 ggcgtgctgg cgtttgtagt gatgattgtg ctgtcgcgcc atccgctggt ggtggaagtg
    19141 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgagttgtt
    19201 gttttcggtt ccgacaacgg ttgccgggtg cagcgtaaaa cgccttatcc gtcctacggt
    19261 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcattgaatg
    19321 ccggatgcgg cgtgaacgcc ttatccggcc tacggttctg tgctcgggtc ttgtaggcct
    19381 gataagacgc gccagcgtcg catcaggcaa aaaacggcaa cgaacacaac aaacacagcc
    19441 tcacctggca tcaaatttat gaggaaaaaa tgaaattact tattctgggc aaccacactt
    19501 gcggcaaccg cggcgacagc gccatcttgc gcggcttact tgatgccatc aacatcctca
    19561 atccacacgc cgaagtggac gtcatgagcc gctatccggt cagttcttcc tggctgctca
    19621 accgcccggt aatgggcgat ccgctgttcc tgcaaatgaa acaacacaac agcgcggcgg
    19681 gcgttgtcgg gcgcgttaaa aaagtcctcc gtcgccgcta ccagcatcag gtattgctct
    19741 cacgcgtcac cgacactggc aagctgcgta atatcgccat cgcccaggga ttcaccgact
    19801 tcgtgcgcct gctgtcaggt tacgacgcca ttattcaggt cggcggatcg ttttttgtcg
    19861 atctctacgg cgtgccgcag tttgaacatg cactttgcac gtttatggcg aaaaagctgc
    19921 tgtttatgat tggtcacagc gtcggtccct tccaggatga gcaatttaac caactggcga
    19981 actatgtctt tggtcactgt gacgcgctga tcctgcgcga atcggtcagc cttgatctga
    20041 tgaaacgcag caatatcacc actgcaaaag ttgaacatgg cgtcgatacc gcgtggctgg
    20101 tcgatcacca cacagaagac ttcaccgcca gctatgccgt tcaacattgg ctggacgttg
    20161 ccgcacaaca gaaaacggtg gcaattaccc tgcgcgaact ggcaccgttc gacaaacgcc
    20221 tcggcaccac tcaacaagcg tatgaaaaag cctttgccgg ggtggtcaat cgcattctcg
    20281 acgaaggcta tcaggtcatt gcgctctcca cctgtacggg catcgacagc tataacaaag
    20341 acgaccgcat ggtggcgctt aatctgcgcc agcacatcag cgatcctgcc cgttaccacg
    20401 tggtgatgga tgaactcaac gatctggaaa tgggcaaaat tctgggagcc tgtgaactca
    20461 ccgtcggtac gcgcctgcac tcagccatta tctcaatgaa ctttgctact ccggcgattg
    20521 ccatcaacta tgaacataaa tccgccggga tcatgcagca gctggggctg ccagagatgg
    20581 caattgatat ccgtcattta ctggacggta gcctgcaagc gatggttgcg gataccttag
    20641 gtcaacttcc ggcgctgaac gcacgactta atgaagctgt cagtcgtgag cgttatacag
    20701 gaatgcagat ggtgcaatct gtgcttgaac gcatcgggga ggtgaaatga aggtcggctt
    20761 ctttttactg aaatttccgt tgtcgtcgga aaccttcgtc ctcaaccaaa tcactgcgtt
    20821 tattgatatg ggctttgagg tggagattgt cgcgctgcaa aaaggcgaca cccagaacac
    20881 ccacgcggca tggacgaaat acaaccttgc cgcaagaacc cgctggttac aggacgaacc
    20941 tacgggcaaa gtggcgaagc tgcgccaccg cgccagccag accttacgcg gcattcatcg
    21001 taaaaatacc tggcaggcgc tcaacctcaa acgctatggt gccgagtcgc ggaacctgat
    21061 tttgtctgcc atttgcggcc aggtcgcaac accatttcat gccgatgtct ttatcgctca
    21121 ttttggccct gcgggggtaa ccgcagcaaa actacgcgaa ctgggtgtca ttcgcggcaa
    21181 aattgccact attttccacg gcattgatat ttccagtcgg gaagtgctca accattacac
    21241 tcccgaatat caacaactgt ttcgacgtgg cgacctgatg ctaccgataa gcgatctgtg
    21301 ggccggaaag ctgcaaaaaa tgggctgccc gagggaaaaa atcgccgtat cgcgcatggg
    21361 cgtagatatg acgcgcttta gcccgcgtcc cgtgaaagcg cccgcaacac cgctggagat
    21421 tatttccgtc gcacgcttaa ccgagaaaaa aggcctgcat gtggcgattg aagcctgccg
    21481 tcagttgaaa gagcagggcg tggcatttcg ctatcgcatc ctcggcattg gcccgtggga
    21541 acgacgcctg cgcaccctca tcgaacaata tcaactggaa gatgtggtag agatgcctgg
    21601 ctttaaaccg agccatgaag tgaaagcgat gctcgacgac gcggatgtct tcctgttgcc
    21661 atcggtaacg ggcgcggatg gcgatatgga aggcattccg gtagcgctga tggaagcgat
    21721 ggcggtcggc attccggtgg tttctactct gcatagcgga ataccggaat tggtggaggc
    21781 cgataaatcc ggctggctgg tgcctgagaa cgatgctcgc gcactggcgc aacgactggc
    21841 ggcgtttagc caactggaca ccgacgaatt ggctccggtc gtcaaacgcg cgcgcgaaaa
    21901 agttgaacac gattttaacc agcaggtgat caatcgagaa ctcgccagct tgctgcaggc
    21961 tttatagagg agaacgtatg ccatttaaaa aactctcccg acgcaccttc ctgacggcaa
    22021 gctcggcgct tgccttcctc catacccctt tcgctcgcgc acttcccgcc cgacaaagcg
    22081 ttaacattaa cgactacacc cctcacgact ggatcgcctc atttaaacaa gccttcagcg
    22141 aagtgcaaac ggtcgtcgtg cctgccggat tggtttgtga caatatcaac accggcatct
    22201 tcatccctcc cggtaaaacg ttacacatcc ttggaagcct gcgcggcaac ggcagagggc
    22261 gatttgtctt acaggacggc agccaggtga caggggagga gggcggcagt atgcataaca
    22321 ttaccctgga tgtgcgtggt tctgactgca ccatcaaagg gctggcgatg agcggctttg
    22381 gtccggtaac gcagatttat atcggcggca aaaacaaacg ggtcatgcgc aacctgacca
    22441 tcgataatct caccgttaac cacgctaatt acgctatttt gcgccaggga tttcataacc
    22501 agattatcgg tgccaacatc accaactgta aatttagtga tttacagggc gacgccattg
    22561 aatggaacgt ggcgattaac gaccgtgata ttttgatatc tgaccatgtc atcgagcgca
    22621 tcaactgtac caacggcaaa atcaactggg gcatcggcat aggccttgcg ggaagcactt
    22681 atgataacaa ctacccggaa gaccaggcag tgaaaaactt tgtcgtggcg aatatcacgg
    22741 gatcggattg tcggcagttg atccatgttg aaaatggtaa acattttgtt attcgtaata
    22801 tcaaagcccg caatatcacg ccggatttca gtaagaaagc gggtattgat aacgcgacag
    22861 tcgctattta cggttgtgac aatttcgtga ttgataatat tgaaatgatt aatagcgccg
    22921 ggatgttaat cggctatggg gtaattaaag gcaaatatct ctcgataccg caaaatttcc
    22981 gagtgaataa tattcaactg gataataccc accttgctta caaattgcgc ggtatccaaa
    23041 tctctgccgg gaatgccgtc tcctttgtgg cactgactaa cattgagatg aagcgtgcct
    23101 cgctggagct tcacaataaa ccgcaacatc tttttatgcg taatatcaaa gtgatgcagg
    23161 aatcctctgt tggaccagca ttgagcatga actttgacat gcgcaaagac gttcgcggca
    23221 tttttatggc gaaagaagaa acactgctgt cttttgcaaa tgttcatgcg gtgaatgaaa
    23281 gagggcaaag ttccgtcgat atcgacagga ttaatcacca tattgttaat gtggaaaaga
    23341 ttaactttag attgccggaa cggagggagt agatttgcga ccattcctgg aaaaatggag
    23401 tcatacttag gaacaatgct actgcaatcc acaacgaagc ggcgtaacat cacaagtaat
    23461 tcagtaatca attcagggta attgatgctg gcgaaaaaaa tcgaacaagc tataattcag
    23521 caaccatttt acaggtggat gaaataatga cgaatttaaa agcagttatt cctgtagcgg
    23581 gtctagggat gcatatgttg cctgccacta aggcgattcc caaagagatg ctaccgatcg
    23641 tcgacaagcc aatgattcag tacattgttg acgagattgt ggctgcaggg atcaaagaaa
    23701 tcctcctggt aactcacgcg tccaagaacg cggtcgaaaa ccacttcgac acctcttatg
    23761 aattagaatc tctccttgaa cagcgcgtga agcgtcaact gctggcggaa gtgcaatcta
    23821 tctgcccgcc gggcgtgact attatgaacg tgcgtcaggg cgaaccttta ggtttaggcc
    23881 actccatttt gtgtgcgcga cctgccattg gtgacaaccc gtttgtcgtg gtactgccag
    23941 acgttgtgat cgacgatgcc agcgccgacc cgctacgtta caaccttgct gccatgattg
    24001 cgcgcttcaa cgaaacgggc cgcagccagg tgctggcaaa acgtatgccg ggtgacctct
    24061 ctgaatactc ggtcatccag accaaagaac cgctggatcg cgaaggtaaa gtcagccgca
    24121 ttgttgaatt tatcgaaaaa ccggatcagc cgcagacgct ggactcagac atcatggccg
    24181 ttggtcgcta tgtgctttct gccgatattt ggccggaact tgaacgcacg cagcctggtg
    24241 catggggacg tattcagctg actgatgcca ttgctgaact ggcgaaaaaa cagtccgttg
    24301 atgccatgct gatgaccggc gacagctacg actgcggtaa aaaaatgggc tatatgcagg
    24361 cgtttgtgaa gtatggactg cgtaacctaa aagaaggggc gaagttccgt aaagggattg
    24421 agaagctgtt aagcgaataa tgaaaatctg accggatgta acggttgata agaaaattat
    24481 aacggcagtg aagattcgcg gtgaaagtaa tttgctgcga atattcctgc cgttgtttta
    24541 tataaacaat cagaataaca acgagttagc aataggattt tagtcaaagt tttccaggat
    24601 tttccttgtt tccagagcgg attggtaaga caattagcgt ttgaattttt cgggtttagc
    24661 gcgagtgggt aacgctcgtc acatcgtaga catgcatgca gtgctctggt agctgtaaag
    24721 ccaggggcgg tagcgtgcgt taatacctct attaatcaaa ctaagagccg ctaatttcac
    24781 agcatgctct gaagtaatat ggaataaaaa agtgaagata cttgttactg gtggcgcagg
    24841 atttattggt tctgctgtag ttcgtcacat tataaataat acgcaggata gtgttgttaa
    24901 tgtcgataaa ttaacgtacg ccggaaacct ggaatcactt gctgatgttt ctgattctga
    24961 acgctatgtt tttgaacatg cggatatttg cgatgctgct gcaatggcgc ggatttttgc
    25021 tcagcatcag ccggatgcag tgatgcacct ggctgctgaa agccatgtgg atcgttctat
    25081 cactggccct gcggcattta ttgaaaccaa tattgttggt acttatgtcc ttttagaagc
    25141 cgctcgcaat tactggtctg ctcttgatag cgacaagaaa aatagtttcc gttttcatca
    25201 tatttctact gacgaagtat atggcgactt accccatcct gatgaagtaa atagtaatga
    25261 agcgttaccg ctatttacgg aaatgacagc ttatgcacca agtagcccat attctgcttc
    25321 taaagcatcc agcgatcatt tagtccgcgc gtggaaacgt acctatggtt taccgaccat
    25381 tgtgactaat tgttctaaca attatggtcc ttatcatttc ccggaaaaac tgattccatt
    25441 ggttattctt aatgctctgg aaggtaaggc attacctatt tatggtaagg gggaccaaat
    25501 tcgcgactgg ttgtatgtag aagatcatgc ccgggcattg tacaccgtta tgacccaggg
    25561 tgtagtaggt gaaacctaca atatcggtgg tcgtaacgag aaaaaaaacc tggacgtggt
    25621 aaatactatt tgcgatctgc ttgatgagat tgtacctaaa caagtgtctt atcgcgatca
    25681 aattatctac gttactgacc gtcctgggca cgatcgccgc tatgcgattg atgcatcaaa
    25741 aatcagcgat gagctgggct ggaaaccgca ggaaaccttt gaatcgggaa ttcgaaaaac
    25801 tattggttgg tatttaaata atttagagtg gtgccgtcgt gtgcaagacg gtagctatca
    25861 acgtgaacgt ttaggattac agtaaatgaa aggtatcata cttgcaggtg gttcaggaac
    25921 tcgtttatat ccagtcacaa tggctgtaag taaacaattg ttgccagttt atgataaacc
    25981 aatgatttat tacccattaa gtaca
//
LOCUS       DAPGWD010000057        25482 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPGWD010000057 DAPGWD010000000
VERSION     DAPGWD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.057"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            214..633
                     /locus_tag="RHX88_002265"
     CDS             214..633
                     /locus_tag="RHX88_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV2230953.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            633..1901
                     /gene="umuC"
                     /locus_tag="RHX88_002266"
     CDS             633..1901
                     /gene="umuC"
                     /locus_tag="RHX88_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDV2230954.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1947..2477)
                     /gene="dsbB"
                     /locus_tag="RHX88_002267"
     CDS             complement(1947..2477)
                     /gene="dsbB"
                     /locus_tag="RHX88_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDV2230955.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2623..4164)
                     /gene="nhaB"
                     /locus_tag="RHX88_002268"
     CDS             complement(2623..4164)
                     /gene="nhaB"
                     /locus_tag="RHX88_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDV2230956.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4386..5105
                     /gene="fadR"
                     /locus_tag="RHX88_002269"
     CDS             4386..5105
                     /gene="fadR"
                     /locus_tag="RHX88_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDV2230957.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5157..6689)
                     /locus_tag="RHX88_002270"
     CDS             complement(5157..6689)
                     /locus_tag="RHX88_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDV2230958.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7019..8317
                     /gene="dadA"
                     /locus_tag="RHX88_002271"
     CDS             7019..8317
                     /gene="dadA"
                     /locus_tag="RHX88_002271"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDV2230959.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     HFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8327..9397
                     /gene="dadX"
                     /locus_tag="RHX88_002272"
     CDS             8327..9397
                     /gene="dadX"
                     /locus_tag="RHX88_002272"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDV2230960.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9784..11520)
                     /locus_tag="RHX88_002273"
     CDS             complement(9784..11520)
                     /locus_tag="RHX88_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDV2230961.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQHHESNISWMFIVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFSLTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11615..12529)
                     /gene="ldcA"
                     /locus_tag="RHX88_002274"
     CDS             complement(11615..12529)
                     /gene="ldcA"
                     /locus_tag="RHX88_002274"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDV2230962.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12629..13240
                     /gene="emtA"
                     /locus_tag="RHX88_002275"
     CDS             12629..13240
                     /gene="emtA"
                     /locus_tag="RHX88_002275"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDV2230963.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13242..13976)
                     /locus_tag="RHX88_002276"
     CDS             complement(13242..13976)
                     /locus_tag="RHX88_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDV2230964.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGVLLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14177..14431
                     /locus_tag="RHX88_002277"
     CDS             14177..14431
                     /locus_tag="RHX88_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV2230965.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISSFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14609..15049
                     /locus_tag="RHX88_002278"
     CDS             14609..15049
                     /locus_tag="RHX88_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230966.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRYKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15128..16825)
                     /gene="treA"
                     /locus_tag="RHX88_002279"
     CDS             complement(15128..16825)
                     /gene="treA"
                     /locus_tag="RHX88_002279"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV2230967.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17146..18564)
                     /gene="dhaM"
                     /locus_tag="RHX88_002280"
     CDS             complement(17146..18564)
                     /gene="dhaM"
                     /locus_tag="RHX88_002280"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDV2230968.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVVPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICLQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            complement(18575..19207)
                     /gene="dhaL"
                     /locus_tag="RHX88_002281"
     CDS             complement(18575..19207)
                     /gene="dhaL"
                     /locus_tag="RHX88_002281"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDV2230969.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19218..20288)
                     /gene="dhaK"
                     /locus_tag="RHX88_002282"
     CDS             complement(19218..20288)
                     /gene="dhaK"
                     /locus_tag="RHX88_002282"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDV2230970.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20516..22435
                     /gene="dhaR"
                     /locus_tag="RHX88_002283"
     CDS             20516..22435
                     /gene="dhaR"
                     /locus_tag="RHX88_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDV2230971.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22535..25402)
                     /locus_tag="RHX88_002284"
     CDS             complement(22535..25402)
                     /locus_tag="RHX88_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2230972.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
BASE COUNT         6288 a         6351 c         6579 g         6264 t
ORIGIN      
        1 tggcgaatgc ttctattcta tttttttgcc gggtgatatt tttcatttct gctggatgag
       61 cgtcgtcgcc agaaggccac gtgagcacag gataagagaa cgaaaaatca gcagtctatg
      121 cagcgacaaa tattgatagc ctgaatcagt attgatctgc tggcaagaac agactactgt
      181 atataaaaac agtataactt caggcagatt attatgttgt ttatcaagcc tgcggatctc
      241 cgcgaaattg tgacttttcc gctatttagc gatcttgttc agtgtggctt tccttcaccg
      301 gcagcagatt acgttgaaca gcgcatcgat ctgaatcaac tgttgatcca gcatcccagc
      361 gcgacttact tcgtcaaagc aagtggtgat tctatgattg atggtggaat tagtgacggt
      421 gatttactga ttgtcgatag cgctattacc gccagccatg gtgatattgt catcgctgct
      481 gttgacggcg agtttacggt gaaaaaattg caactacgcc cgacggtaca gcttattccc
      541 atgaacagtg cgtactcgcc cattaccatc agtagcgaag atacgctgga tgtctttggt
      601 gtggtgatcc acgtcgttaa ggcgatgcgc tgatgtttgc tctctgtgat gtaaacgcgt
      661 tttatgccag ctgtgagacg gtgtttcgcc ctgatttatg gggtaaaccg gtggttgtgc
      721 tatcgaataa tgacggttgc gttatcgccc gaaacgctga ggcaaaggcg ctgggtgtta
      781 aaatgggcga tccctggttc aaacaaaaag atctgtttcg tcgctgtggc gtggtttgct
      841 ttagcagcaa ttatgagctt tacgcagaca tgagcaatcg ggtgatgtcg acgctggaag
      901 aactatcgcc ccgcgtcgag atttacagta ttgatgaggc attctgcgat ctgacaggtg
      961 tgcgtaattg tcgcgatctg actgattttg gcagagaaat tcgcgcaacg gtgctacaac
     1021 gtacccatct tactgttggt gtggggatcg cccagaccaa aacgttggct aagcttgcca
     1081 atcatgcggc aaaaaaatgg cagcggcaga cgggtggggt ggtggattta tcaaatctgg
     1141 aacgccagcg taaattaatg tctgctctcc ccgtggatga cgtctggggg attggacggc
     1201 ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt tctcgatttg gcggatacag
     1261 atatccggtt tatccgtaaa cattttaatg tcgtgctcga aagaacggtg cgtgaactgc
     1321 gcggcgaacc ctgtttgcaa ctggaagagt ttgcaccgac gaagcaggaa attatctgtt
     1381 cccgctcgtt tggtgaacgc atcacggatt atccatcgat gcggcaggcc atttgtagtt
     1441 acgctgcccg ggcggcggaa aaacttcgca gcgagcatca atattgtcgg tttatctcca
     1501 catttattaa gacgtcacca tttgcgctca atgaacctta ttacggcaat agcgcgtcgg
     1561 taaaactgct gacgcccact caggacagca gggatatcat taacgctgct acgcgatctc
     1621 tggatgccat ctggcaagcg ggccatcgct accaaaaagc gggcgtgatg ctgggggatt
     1681 tcttcagtca gggagtcgcg cagctcaatt tattcgatga caacgcaccg cgccccggga
     1741 gtgagcaatt gatgacggta atggatacac tgaatgctaa agagggcaga ggaacactct
     1801 attttgccgg gcaggggatc cagcaacaat ggcagatgaa gcgtgccatg ctttcaccac
     1861 gttatacaac gcgaagttct gatttactgc gggtcaaata aatatagcgg caggaaaaaa
     1921 gcgctcccgc aggagcgctg aagggattag cgaccgaaca ggtcacgttt tttcgcttta
     1981 aacggctggg aaatcaccac cagcaccgcg acaatcaggt aagcgataaa aataccgagc
     2041 agccactgcg gcatttccat acctaaaaaa tcccactgac gctcggcgca atcgccagag
     2101 gcgacaaaca cttgcggcac ccatttatcc agcggcagcc attccgggaa acgaaccata
     2161 aaatcacagg tggcaaacgg cgaaggatag agctgaagca tggtgtgctc gtaagttaac
     2221 tgcacaccgc ggaacgcact atacaaccag ataaccatcg ctacataacg cagcggagtt
     2281 ttcggggcga tcgcgccaat cagcgcagca cccagaacgc cgaataacgc gcagcgttca
     2341 taaatacaga gcacgcaagg tttcagtaac atcacatgct ggaaccacag cgccgtcagt
     2401 tccagtgcca gagcagtaaa cgccatcaac agccacgcgc cccggccttg tgaacattgg
     2461 ttcaaaaatc gcaacataat catttccctg caatatgcat agagtgcgca gtttaaacca
     2521 attcattcgc tgcgccacca ggaggcagca aaaataggac gcaattggac aattatcggg
     2581 cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt tatcaaagcg ttgctatcca
     2641 gcccatttgc ataaaccatt cggttacagg ggcaagcgta aactcgacgc agagcaagcc
     2701 gacgagtgtc aggacgaggg tgtaaggcag ggccatccac accatgcggc cataagagag
     2761 gcgaatcaat ggcgcgagtg cagaggtcag caggaacagg aacgcagcct gaccgttcgg
     2821 cgtagcgaca gagggcagat tggtaccggt attaatggcg accgccagca gctcgtattg
     2881 cttcaacgtg atagcgccac tttccattgc cgctttcgct tcgttgatat aaatcgtccc
     2941 cacgaagacg ttatccgaaa tggatgacag cagaccgttg aaaatataga acagcgacag
     3001 ctgagcatgt tcagatgcct gcaacacaaa ctgaataatc ggcgaaaaca gttgttggtc
     3061 gataatcacc gcgactaccg agaaaaacac cgtcaacagt gcggtgaatg gcagagattc
     3121 ggtgaaggct ttaccgatag catgttcatc ggtgacaccg gtcaatgatg tcgccagaat
     3181 aatgactgac aaaccaatca agccaacttc cgccagatgc aacgccagcg cagtcaccag
     3241 ccagacgcca ataatcgcct ggacaatcag acggatttta tcctgacggg tacgctggtg
     3301 gcggctttga tcgtcaaact gttgcaacac ttcgcggact ttctccggca gcgtttcacc
     3361 gtagccaaac caacgcagct tctctaccag caggcaggtt aacaggccac aaatcagaac
     3421 cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca aaatgccagc cagccgcttt
     3481 agcgatgatc aggttctgtg gttcgcccac catggtcatc acgccgccta atgcagtgcc
     3541 gacaccggca tgcatcatca ggctacgcag aaagccacgg aactgttcca gaaccacttt
     3601 gtaatgcttg tcgatatgac tatcgtcttg caggtcggta tcttcggtac gggaagaggc
     3661 tacgcggtga taaataccat aaaaaccgac agcaacgctg atcaccaccg ccacgacggt
     3721 taaggcatcg aggaacgcgg agaggaacgc tgccgccacg caaaaagaga gcgacagcag
     3781 cattttggag cgaatgctta acagcaaacg ggtaaatatg aacagcaaca gctgtttcat
     3841 aaaatagata cccgccacca taaacatcaa taacagcaag acttcaagat ttgccgccac
     3901 ctcttcacgg acgtgttccg cgctggtcat gccgatgaat accgcttcga tagccaacag
     3961 accaccgggg agcagcgggt agcatttcag ggccatcgcc agagtgaaaa taaattccgc
     4021 taccagcaac cagcccgcga cgaaagggct gatgaggaaa attaacgggt ttacgattaa
     4081 gaaaatgatg agggcgagtt tgtaccagtc gggggactgg cccaaaaaat tgcgccatag
     4141 cgcgcggccc caggagatct ccatgatggt ttcccttacc ttacaaataa tcaatgatgt
     4201 ttttatgttt aaacgcaaag cttaacggtc aggcaggagt gaggcaagtc ttgatagtca
     4261 aggggaaaga gatgcggaaa atgaagcctt gatccctttt ttcttctttt tgtctgctat
     4321 cagcgtagtt agccctctgg tatgatgagt ccaactttgt tttgctgtgt tatggaaatc
     4381 tcactatggt cattaaggcg caaagcccgg cgggtttcgc ggaagagtac attattgaaa
     4441 gtatctggaa taaccgcttc cctcccggga ctattttgcc cgcagaacgt gaactttcag
     4501 aattaattgg cgtaacgcgt actacgttac gtgaagtgtt acagcgtctg gcacgagatg
     4561 gctggttgac cattcaacat ggcaagccga cgaaggtgaa taatttctgg gaaacctctg
     4621 gtttaaatat ccttgaaaca ctggcgcgac tggatcacga aagtgtgccg cagcttattg
     4681 ataatttgct gtcggtgcgt accaatattt caactatttt tattcgcacc gcgtttcgtc
     4741 agcatcccga taaagcgcag gaagtgctgg ctaccgctaa tgaagtggcc gatcacgccg
     4801 acgcctttgc cgagctggat tacaacatat tccgcggcct ggcgtttgct tccggcaacc
     4861 cgatttacgg tctgattctt aacgggatga aagggctgta tacgcgtatt ggtcgtcact
     4921 atttcgccaa tccggaagcg cgcagtctgg cgctgggctt ctaccacaaa ctgtcggcgt
     4981 tgtgcagtga aggcgcgcac gatcaggtgt acgaaacagt gcgtcgctat gggcatgaga
     5041 gtggcgagat ttggcaccgg atgcagaaaa atctgccggg tgatttagcc attcaggggc
     5101 gataatccct tccgtttaaa gagcaaaccc ctcaaacgag gggttttttg ttgtttttac
     5161 agatttccca ttcttggcgg gcaacgttcc agcaactcga tgctgccgtc ttcgttttgc
     5221 tgttcgagca tcacatcaaa tccccacagg cgatgcacat gcttcagcac ttctttgcgc
     5281 ccccgatcca gcggtgcgcg attatgtgga atgtaacgca gcgtcagcga acggtcgccg
     5341 cgcaaatcca cgttccagat ctgaatattc ggctccagat tacttaagtt atattgcgac
     5401 gataaccggt tacggatctc ccgataacct tcttcattat gaatagcgga aatctccaga
     5461 taattatgcc gatcatcatc cagcacggtg aagaagcgga aatcacgcat cactttcggt
     5521 gacagaaact ggctgataaa actctcatct ttgaaatcac gcatcgcgaa atgcagcgtt
     5581 tccagccagt cggaaccggc gatatccggg aaccagtatt tgtcttcttc cgttggcgac
     5641 tgacaaatcc gtttaatatc ctggaacatg gcgaacccga gggcatacgg gttgatgccg
     5701 ctgtaccacg ggctgttata ggggggctgg aagaccacat tggtgtggct gtgcaaaaac
     5761 tccagcataa aacgttccgt tactttccct tcatcataca gatggttaag gatggtatag
     5821 tgccagaagg tcgcccagcc ttcgttcatc acctgagtct gtttttgcgg ataaaaatac
     5881 tggctcacct tacgcacaat acgcaggatt tcacgctgcc atgattccag cagtggcgca
     5941 tttttctcca taaaatagag caggttttct tgtggttcgg acggatagcg gcgcgcttca
     6001 gcaaccgttt tctcttcctc gcgcttcggc agggtacgcc agagcatatt gacctgactt
     6061 tgtagatact cttcgcgact tttctgccgg gctttctctt cttgcagcga gattttttgc
     6121 gggcgtttgt accggtccac gccgtagttc atcagcgcgt ggcacgagtc cagaagccgt
     6181 tctacttcat caacgccata acgttcttcg cactcggtaa tatatttacg ggcaaaaatc
     6241 agataatcga caatcgaact ggcgtcggtc caactgcgga ataagtaatt gtttttaaag
     6301 aaagagttat gcccatagca ggcgtgtgcc atcaccagcg cctgcatggt aatggtgttc
     6361 tcttccatca ggtaagcgat acacgggtta gagttaatga cgatttcata ggccagtcct
     6421 tgctgaccgt gcttatacag ccgttcagtc tcgataaact ttttaccgaa tgaccagtgc
     6481 ggatagttaa ttggcatgcc gacgctggag taggcatcca tcatctgttc tgaggttatc
     6541 acttcaatct ggtgcgggta ggtatccagc cggtagagtt tcgccacccg gtctatctct
     6601 gccagataaa catccagcag gtcgaacgtc cagtcgggtc catcgctcaa acgtgtggtg
     6661 tccttattca tagaatcgat cgtcgccata cgcgcacctc attgttgtcg gcgctctctg
     6721 tgtggagcac ctcatttcaa gcatagaaca cctgttaaaa accgcgtcgc cggagaattt
     6781 ttttctttgc gatttcttat tatcagagtg ccactaatcc gcttctgaac ggaattttat
     6841 gctggataaa aagggcgttc agcaggagat actaaagacg ccatattgcc gcagagtcag
     6901 ggagatgtga gccagctcac cataaaaaag ccgtatgttg aataatattt tcaactgagt
     6961 tatcaagatg taattagatt attattcttt tactgtatct accgttatcg gagtggctat
     7021 gcgagttgtc atactgggaa gtggtgtggt aggcgttgcc agcgcctggt acttaaatca
     7081 ggcaggacat gaggtcaccg tcattgatcg ggagccgggg gccgccctgg aaaccagcgc
     7141 cgcgaatgcc gggcaaatct cccccggata tgctgcaccg tgggcggcac caggtgtgcc
     7201 tttaaaagcg attaaatgga tgttccagcg ccatgcgccg ctggcggttc gtctcgacgg
     7261 tacgcagttc cagttgaaat ggatgtggca aatgttacgt aactgcgaca ccagccacta
     7321 catggaaaac aaagggcgga tggtgcgtct ggcggaatac agccgtgatt gcctgaaagc
     7381 attgcgcgcc gaaaccaata ttcagtatga agggcgtcag ggtgggacgc tgcaactgtt
     7441 ccgtaccgaa caacagtatg aaaatgcgac ccgcgatatc gccgtgctgg aagatgccgg
     7501 cgtaccgtat cagctgctgg aatccagccg cctggcggaa gtggagcccg cgctggcaga
     7561 agtagcgcac aaactgacgg gtggtctgca actgcccaat gatgaaaccg gagactgcca
     7621 gctgtttacc cagaatctgg cgcggatggc agagcaggcg ggggttaaat tccactttaa
     7681 tacgcccgtt gaccaactgc tttgcgacgg cgagcaaatc tacggcgtga agtgtggcga
     7741 tgaagtgatt aaggccgatg cgtatgtgat ggcgtttggt tcttactcga cggcgatgct
     7801 caaaggcatt gttgatattc cggtgtatcc gctgaaaggc tactcgctga ccattccaat
     7861 tgcgcaggaa gatggtgcgc cggtatccac cattcttgat gaaacctaca aaatcgccat
     7921 tacccgtttc gataaccgca ttcgtgttgg cggaatggcg gaaattgttg gttttaacac
     7981 cgagctgttg caaccgcgtc gtgaaacgct ggaaatggtg gttcgcgatc tctatccacg
     8041 cggcggtcat gtcgagcagg cgactttctg gacgggtttg cgcccgatga cgccagacgg
     8101 tacgccggtt gtcgggcgta cacgctttaa aaatctgtgg ctgaataccg gtcacggcac
     8161 gctcggctgg acgatggctt gcggttccgg tcagttgtta agcgatctgc tctctggtcg
     8221 cacgccagcg atcccatatg aggatctaag cgtagcgcgc tacagccgtg gatttacgcc
     8281 atcacgtccg ggccatttac atggcgcaca cagctaagga aacgagatga cccgtccgat
     8341 acaggccagc ctcgatctgc aggcattaaa acagaatctg tccattgtcc gccaggccgc
     8401 gccgcacgcg cgcgtctggt cggtggtaaa agcgaacgct tacgggcatg gtattgagcg
     8461 tatctggagc gcgctcgggg ccaccgatgg ctttgcatta cttaacctgg aagaggcaat
     8521 aacgttacgt gagcgcggct ggaaggggcc gatcctgatg ctggaaggat ttttccatgc
     8581 tcaggatctg gagatttatg accagcaccg cctgaccacc tgcgtacaca gcaactggca
     8641 gctcaaagca ctgcaaaatg cgcggctaaa agcaccgttg gatatttatc ttaaagtgaa
     8701 cagtgggatg aatcggttgg gcttccagcc cgatcgcgtg cttaccgtct ggcagcagtt
     8761 gcgggcaatg gcgaatgttg gcgagatgac cctgatgtcg cattttgccg aagcggaaca
     8821 tcctgatgga atttccagcg cgatggcgcg tattgagcag gcggcggaag ggctggagtg
     8881 tcggcgttcg ttatccaatt cggcggcgac tctgtggcac ccggaagcgc attttgactg
     8941 ggttcggcct ggcattattt tgtatggcgc ttcgccgtcc ggtcagtggc gtgatatcgc
     9001 caataccgga ttacgtccgg tgatgacgct aagcagtgag attattggtg tccagacgct
     9061 aaaagcgggt gagcgtgtgg gctacggcgg tcgctatact gcgcgcgatg aacagcgaat
     9121 cggcattgtc gccgcagggt acgccgacgg ttatccgcgc cacgcgccta ccggtacccc
     9181 tgttttagtg gacggcgtgc gcaccatgac ggtggggacc gtctcgatgg atatgctagc
     9241 ggtcgattta acgccttgcc cgcaggcggg tattggtacg ccggttgagc tgtggggcaa
     9301 ggagatcaaa attgatgatg tcgccgccgc tgccggaacg gtgggctatg agttgatgtg
     9361 cgcgctggcg ctacgcgtcc cggttgtgac ggtgtaactt gttgtaagcc ggatcggagg
     9421 caacgtcttc tgggtgcaaa aaaaatcatc catccggctg gtcagcaact gtagttgtta
     9481 atgtgacaga gccattgccc atgatagtgt ccattaaaag gatggacact atttccccgg
     9541 aacctgaact caccgcacag gcgttctaca taaaacgctt acgcttcatt gttgactcgg
     9601 agtcgacttc agataaatca cgctttaccc ttgatggagc ctgtacatag atttgtgtaa
     9661 ttgcctgatt ttgatatgtt caattcaaca tcaaatgaag gttaaattat ggacgacaaa
     9721 caattgcagg ctcaggctgc gttcagcaaa gcatcgcaac cggcgataga tgcttcatta
     9781 aatttaagat tcagcttcct cttcagccac ccgtacgcca atcttcaaca cttcattatc
     9841 ttctttctcg gccaccgtcc agatcatccc ggcaaactct acctggtcac cgacaaccgg
     9901 tgccgcgcct aacaactgct ggacaatttc acccagcgtt tgctgcttat cacgatattc
     9961 tcgcccgtct tccaggccat atatcagcgc aacatcagca tatttggcgc tggcttcgag
    10021 aatgaagtca ccaaagaagc gttgatccag cgcgaccggc ggcgactggc tgaacagttt
    10081 accgagcgcc ggaagatcgc gttcccgacc aattacacac aacacatcgc cttcgcgcag
    10141 tcgggtgctg ccggtaggat gaagcaactg gttatcacga aacagtgccg caatacgcgt
    10201 ctcttttggc atatgcaaat cgcgcagtgc cgcgcccacg caccatttat cggcactcaa
    10261 ttgataaaca aactgctccc acggattttc cggatgaata tccaggccaa cgcgtgacac
    10321 cggacgtccc actggcggaa ccaccacttt ggctttttta gccgcccacg agagtgatgt
    10381 tccctgcaag agcagtgaaa ccagaaccac aaagaaggcg acattaaaga acagacgtgc
    10441 attctccagc cccgccatca tcgggaacac ggccaggatg attggcaccg cgccgcgtaa
    10501 tcctacccag ctgataaaca cgcgctcacg cagattgaaa ccgcggaagg ggagcaatcc
    10561 agcaaatacc gaaaggggac gggcgaagaa tatcatccat gccgacaaaa tgagcgccgg
    10621 aatggcaata ggcagcagat cgcttgggtt aaccaacagc cccagcacca ggaacatggc
    10681 gatttgcgcc agccaggcga ggccgtcgaa attttgcagg atgccgtagc ggttgcgaat
    10741 agggcgatta cccagcagaa aaccgcacag atacaccgcc agaataccgc taccttccag
    10801 cgcagtagtt aatgagaaaa tcagaatacc gccacttaat gccagcaatg gatataatcc
    10861 ggcgggcagg gcgatgcggt tgatcatttg cagtagtaaa taaccgccgc caagacctat
    10921 tacaatgccc agaccaaatt gttgcagaat atcgacaatg aacatccagc taatattgct
    10981 ctcatgatgc tggatcatcg caatcagggt aatcgtcaga aagaccgcca ttggatcatt
    11041 actgccggat tcaatttcca gcgtcgagcc aacacgttcg ttaagcccct taccacccag
    11101 cagagaaaag accgctgcag catcggtaga gccgacgata gcgccgatta ataagccttc
    11161 aatcaaatca agattaaaca gccacgccgc catcatgccg gttaaaccag aggtgataag
    11221 cacgcccagc gtcgccagcg acagtgccgg tcctaacgcc acacgaaagg aactggcctg
    11281 agtgcgcatc ccgccgtcga gcaaaataat cgccagtgcc aggttactta ccatgtaggc
    11341 gaaggggtaa ttatcaaacg ggataccgcc gacgccatcg actcctgcca gcatgccgat
    11401 cgccagaaaa ataaccagaa taggaatgcc aagacgggaa gaaaatgaac taagtaatat
    11461 actgctggtg acaaggatgg atcctaagat gaaaaggcta attattgttg tggcatccaa
    11521 cggtcggtta ctcctgatta cgctgtctct tatataaacc ctaccatatt agcggcagag
    11581 acagcgtttt acttagccct gaagcgtgat ttttttacat tttaagaaca ggatgaccgc
    11641 taatcgtcaa ctgagtgcct tcccgggtgt tattcagaat ggcatgtgcg cccaacggca
    11701 gcgtaaccgt gcgttgttca tgaccaaaat cgaggccggt aatgagtgga atcgacaggc
    11761 gggaacgcaa aaacgcgtac actgactcca ggttgtaacc tgcgtcataa tcattgggcg
    11821 tgctgccgct aaagctaccg agaataatcg ccttctgacg tggcaaaatt cccgcatgat
    11881 agagctgcaa cagcatacgt tcgacccgga aagggtgctc gttaatatct tccagcacca
    11941 gaataccgtt ctcaattttt ggcatccacg gtgtaccgat cagtgaaatc agcatcgcaa
    12001 gattgcctcc ccacaacgtg ccttcggccc gacatgtcgg gccttcccct tgccattcaa
    12061 tggtgaaggt ttcattgcgt aacgccagcc agaaatggtg ctcggtaaag gcgttcagtt
    12121 catccgcgcc aaagtttgct accagcatgg ggccgctaaa ggtgatgaca ttgccatgcg
    12181 ccagaagacc gcactgaatg gcggtaaaat cgctatgtcc gcaaatgagc aacgggtcat
    12241 gttgttggcg agccaccagt gcctgccagt caatatctgc cagtaaacga ctggcaccgt
    12301 aaccgccgcg tacagccagc acgatggtgt tgggggtagt cagtctggca agggaattga
    12361 gatcttccag acgctctgtt tccgtacccg caaaacgctc acaacgacgg gcaataacct
    12421 cgacgttatt gacttgatgc cccgcgtcgg taaggcgctg gataccacgc agcgcggcgt
    12481 gctgtttaat gcagtaaccc gatggggcaa ttaagtgaaa cagagacatg gcaattcctt
    12541 gctgacaaca gaaacgaaat gtatatcatg ccgcttaggt gtgccgttgt cacctcaacg
    12601 gcgattccag gctataagga tagaagaagt gaaattgaga tggtttgcct ttttgattgt
    12661 gttattagcg ggttgttcat caaagcatga ctatacgaac ccgccgtgga acgcgaaagt
    12721 tccggtgcaa cgtgcgatgc agtggatgcc aataagccag aaagccggtg cagcctgggg
    12781 cgtcgatcca caattgatca cggcgattat cgctatcgaa tcgggtggta atccgaacgc
    12841 ggtgagtaaa tcgaatgcca ttggtttgat gcagttaaaa gcttcaacct ccggacgtga
    12901 tgtttatcgc cgtatgggct ggagtggtga gccgacgacc agcgagctga aaaatccaga
    12961 gcgtaatatt tcaatggggg cggcttacct gaatattctg gaaaccggcc cgctggcagg
    13021 cattgaagat ccgaaggtac tgcaatatgc gctggtggtg tcatacgcta acggggcagg
    13081 tgcgctgcta cggactttct cgtcagatcg gaaaaaggcg atcagcaaaa tcaacgattt
    13141 ggatgctgac gagttcctcg aacacgtagc gcgaaatcac cctgcgccgc aggctccgcg
    13201 ctatatctac aaacttgagc aggcactgga cgcgatgtaa atcagtcgcg cactttgtcc
    13261 gctttttccc gggcttctcg ctcgagagag aaaataatcc gctgtaattg ccgctccacc
    13321 gtcgggctga cgttaagaaa acggaagctc agacggggag tggtgatggt ttcattcttg
    13381 ccatcaatca ctttgcgctc gctgatggag attaactggg cgtcaaagtg aaaaacaccc
    13441 cattgcccca tgttgacttc aatctgagcg aagcgcatgc cttcttgtaa ttcggcaggc
    13501 tttgctgttt ccagtaatac gcccatgccg cctaacgaca aatcatacag gcggaaacgt
    13561 aacgtactgt tatccgccag tttggtctgg caaaaataag gcggatggag tggggcggag
    13621 atgcggaaat atcggcgtcg ttgtacaaac cataaggtgg gaggcggtac ggtaataaat
    13681 gccggaagct gcaagtattc actctgctgt agttgttcaa cagtaaactc gactttcgca
    13741 ccctgagttt cggcggtaat ggtaatgtgc tgtgccttta gcacggcgat gttgtcttcg
    13801 gcttgactgc cgaaatccag caccagttta tccggggtta ttgccagtat tttgctgatc
    13861 agctgcccgc cattccaact gagacgcaaa ggaattgcgg ctttgtgcaa atcgcgtaac
    13921 acgcccagga cggctaacgg attttgtttc aggaactgct catggtaatg actcacgcgg
    13981 aaaaactcct gactgacaaa ctgtctttgg tttatcggtc acagttaaca aaacttaata
    14041 caaatgcgtg aatatttttt acatgttgtt cttaaatcag ccgacatacg cccagcatcg
    14101 atcccgccct tgcctatact tagagcgttg atgtaagcat ttcttgcgtc cgattcatcg
    14161 aaacgagggc ctgaacatgg gaattattgc ctggattatt tttggcctga tagccggcat
    14221 tatcgccaag ctaatcatgc cggggcgtga tggtggtgga tttttcctga cctgtattct
    14281 cgggatagtc ggtgcggtgg tcggcggctg gctggcgacc atgtttggca ttggcggctc
    14341 catcagtagt tttaatctgc acagcttcct ggtggcggtg gtgggagcta ttctcgttct
    14401 gggcatattc cgcctcctgc gaagagaata agattttcat aaggcggata gcgatacaga
    14461 tgccgctatc cgctttcaca tcagaacgta tactcgacac ctgctttacg ggtgaaaaaa
    14521 atcaatatag cacttcgata tccgataaag ccggttcccg gtaacaacaa cagaatcctt
    14581 ttttaattat tgtttcgttg tttttggtgt gattcagaaa ataaatattt tagacagtag
    14641 ccagtcaggc gttaaaaatg gggcggatag tgaggcggtt ttggctgcgc ttattgaaca
    14701 tttagaattg atcaatcctt ctggcagatt aagccaaaat acccgttcgg caatgcttca
    14761 gttacgcgaa gaggaatggt ctgaattctt tttttggttg ctaaattctc tggagtgtct
    14821 ggattatgtg attattaatt taactccaga aagcaaaaaa acgttgatga gtgaacaccg
    14881 taacaatatt caggtagcaa tcgatgcttt atataggcaa cgtcggtata aatcaccggg
    14941 ggatgatagt gaaacactta cccgtagaaa tgacgctatc ttcggcaacc acgtatggca
    15001 aacatttgcg caatacttcc cgccaggact tgaaaaaccg tctgtgtaga aaacaccata
    15061 gctatggtgt tatctgccga caacgccatc cggagaagcc gaatgacgtt gtcggagtaa
    15121 gcgctggtta aggtgtgggt tgtgcctctt tggttgaggg ttgcgtcgtt gctgacttaa
    15181 cggtcggacg cgtcgccgga acattgtcac acggttgctc tttcgggcag atcaaatcca
    15241 gcattttcag cgtcacgcca ttggtccagc caaagccatc ctgtaatgga tattcgccac
    15301 cgccgccccc cgttccggtg gtgctgacat catatttttc caccagcttt ttctcccggt
    15361 cataggtgtg ctgaacattg gtcaggaagt gccagctaat gtccatcgcc acctcttttt
    15421 gcccgtagtt ttgtaatcct tctgtcgcga cccactgtaa cggtgcccag ccatttggcg
    15481 catcccattg ttgcccactt ttcaccgacg tggtgttcag gccgccgggt tgcagcagat
    15541 gtgttttcgt cgccgtcgcc attttgttgg cgcgatcttt cgctgccgca ttgacgtaca
    15601 gcgggaacag ggcggccgcg gttaactgat tgcgcacttt atgacttttc aggtcgtaat
    15661 cggcatacca gccttgttga tcgttccaca ggtatttttc gatccctttt tgacgggcat
    15721 ttgccagcgt ttcgtactgg tttgccatcg cgttatctcc ggcagctttg ctggcgcggg
    15781 cgaggatttt ttccatttta aacatcaggc tgttcagatc gaccggtacg atgctggtgg
    15841 tgcgtaaggt atttaactgc tgcgggttgt ccatccagcg cgagctgaaa tcccagccag
    15901 acgcagcggc agagcgcagg tcgcggtaaa tttcagtggc aggtcgattc ggattgcttt
    15961 tggcggtggc aatatcttcc acccatgact ctggtcgtgg cgtatcgcga tcgtcccagt
    16021 agcggttgag aagggtacca tcctgaagtt ttacaacgcg tttttcctgt tgtccggctt
    16081 gcaggttttc aacaccgtcc atccagtaag catattcttt ttgcatttgc ggcaggtatt
    16141 gcttcaacgc ggcatcgcct tcatgctgcg ccagtaactc taccatcagg gcaaagaagg
    16201 gcggttgcga gcggcttaaa tagtaactgc ggttgccgtt gggaatatga ccgtaagtgt
    16261 ctatttcatg agcaaaattg gccaccatat ccgcgacttt atcccagtga ccgctttcgg
    16321 caagtcctaa catggtgaag taactgtccc agtaatatac ctcgcgaaaa cgtccgcccg
    16381 gcacgacata aggttccggc agcggtaaca gagaatccca tttttcggta ttttcggtag
    16441 aacgcgttaa taccggccaa agtccgtcaa tatgttcgcg tagtgattgc ccctctggcg
    16501 gaacatattt ctcgccctct ttcggcaggg tgaaattgac gttaacgaaa tggcgcagat
    16561 caaatccgct ctggttttgc tgcatccgat aatcagcaag gatcatcagc ggatcgctgt
    16621 tcggcacggc atcggcaaag gttttttggt ccggaaaaag tttggcgttt tgcacatcat
    16681 taaacagcgg ccctaataaa atatcaggcg gctgtggcgt taccggtgtt tcttctgcct
    16741 gcaccgatag cgcagcgaaa cacaaaaaga tgcaggctgg aattaacgcc attttttgcg
    16801 ggcgagaagg tgcgggggat ttcatcaatc attctccttt ggcgaaaccg aaaaaaacgg
    16861 tattcaagta tcagaaaacc ttagttcagg atcgcactag ctgcatgatc gaaatctcat
    16921 tctgcgaact gtcaggcatt ttagaaaagg gaattaaagg gataaataac ggcgaaatag
    16981 agtgattaaa taacgtctgt ttattagcca gccagcgacg ttggggtcgc gattagcgca
    17041 gaaaaaatca tcatgatttg gtaaaaaata taacaggtag ttaaaaaact tccgtgcaga
    17101 gtaaatagcc ggatggcatc agaacgatgc catccgaacg gtggtttaac cctgacggct
    17161 gaaacgttgc gttttaacgt ccagcgttag cgtttcttct ggttgtatcg catacagttt
    17221 ctcaccctgc aggcaaatcc agccaatccc cagttcacgg gcgatcaggg cgctgtggga
    17281 tagcggactt ccggcgctaa ggcagatacc ttttacaacc gccggatcca gttgcagtat
    17341 tgtggaggga tagatgttct ccgccagtag aatagttggc gagttaaact gcgggagttc
    17401 ttctttcgtt tgggtcaggt ggaccagggt gcgatgcaga agatcgtcca catcaatata
    17461 gcgagcttgt agatattcat catccagttg ctggtattgc tggctaagtt ctttaagaac
    17521 ttgctgccag gcatattctg ccgtgcaatg ttcatgctga aggagttcgc ttgccgccgc
    17581 cagcagttcc ggatcatcta acagtgtatg gtgaccagaa aagattgcgg caatatcgtc
    17641 aagcccgctg gcttctgctt tcgctgttaa cgtcatcaga tctaataacg tgaagtcaat
    17701 ggcctggcgt aatcgttctt gttcttcttc cacggtcagg gtcgattttg cctgtaccgt
    17761 acataaaact ggttgataat aaaaggcttt acccgaaaca ggcggaacgg gacgcagagt
    17821 aggtggaaca acttcctccg tttcaccaaa gttatcttca gccagctgac ggaaagcgat
    17881 cagtgcctct tcagcttctg gccctttcgc aatcaggcgc agcgtatcgt tatagcgaac
    17941 ttgtagtagc gcaatctggt taatgctctc cggtgtgacg catttgccgt ttttctccag
    18001 caacatatcg gcattaaatg tcgataaggt ataaaccagc cgggaggccg gacgtacatg
    18061 caggccgtta cggtttttta tgaccaccgc cagagaacgg gcttcttcat cgtacggagg
    18121 acatgtgtca gagatttcag tgtcggagga cggtaaaccc agttgttcac gtttggcttc
    18181 cagcgcatgc atagcgtcaa agataacttt gtcgatatcc gcccccgagg ccgcgctgac
    18241 cgttgctgcc agtgtacctt cgaccaacgg cgcagcacac aaacgtactt ttgcggcgat
    18301 cttgggagcc agcaattcca gcgcagtttc agcactcaat aatgcgctac ccatatccat
    18361 catgaccagc acatggtcgg catcagcaac agattcgatg gcctccatca ctttgacggc
    18421 atcggtacca ataggatttt gtggatcgtc aattcccgcg gcaatggcga ttttacaact
    18481 atcactcatt aacatctgac gggctaattc accgacacct tctcccagtc ggctgctatg
    18541 tgaaactatg accaggttta ccatcaccaa ttccttactc ttttgcggct aacgccaaca
    18601 tttgcatcat aaacatcacc gaggtcgcgc cgggatcctg gtgaccaata ctgcgttcac
    18661 cgagataact ggcgcggcct ttgcgggctt gcatcgtaat cgtactttgt gcagcggatt
    18721 cggcgattct actggcagct tcgagcgcca ccggaacaga gagattttgc tcgctggact
    18781 gacgtaacga ttccaccacc ggcacccaca catcacacat ggttttatcg ccaggttcgg
    18841 ctttcccgcg actgattacg ccgtccgcgc catcgcggaa catctgataa agctcttcca
    18901 gtgtcaggct ttgccgtgcc tgggtcgcct gtgcggcgcg gataaagaag gtaccgaaca
    18961 gcggaccact ggcaccaccg acgctggaaa gcagcgtcat accggtattc ttgagaatga
    19021 aaccgatatc tttatctgcg atagcaggga gtttttccac cactttgcta aagcctcggt
    19081 tcatatttag cccgtggtca gcgtcgccaa tttcgcgatc cagtccggta agatactcgc
    19141 tctcggtgct gaaaatatcg ccacaacgag tgagccagtt aacaatttga gttctgctca
    19201 gtgacattgc tttctcctta tttaccccag ttaagggccg gggtgtggac cggggcgtcc
    19261 cagagtgcca gcgtttcgtc atcaactttc agtaaggtga ttgagaaacc ggtcatatcc
    19321 agtgaggtgc agtacgcgcc aattaaatta cgttcgatag tcaatcccgc ttgctggcaa
    19381 cgtgtggtca ggcggttata gacgccgtac agctcagaaa gcggagttgc gccaagattg
    19441 ttaaccagcg caatcacccg atcgccagac tggagcggtt gtttggtttg ttgttcttcc
    19501 tgccaactgc cttgttgata atcccagaaa cgcaaagtgc gatggtatga gccatttacc
    19561 agcagggtgt cgaacatttc atcgacggtt tgatcaaggg aagagaaggg gcggcggtca
    19621 atacccggct caccatgaat gccgacgcca aactccatct cattatccgc cagggtaaaa
    19681 gaaggtttgc ccgcggcagg aacggtacag gcaccgagag cgatacctat tgagtggcct
    19741 tgattattca acttacgccc cagttccgca caggcgtcca gtgagtcgcc acgctccgcc
    19801 gctgcgccta cgagtttttc aattaatacg gtgttggcaa cgccgcgtcg cccggcagta
    19861 taaagactgt cttttaccgc aacgtcgtca tcaatgacca cagtggtcac ttttacgccg
    19921 ctatcgtgca gtaactcggt cgctgtttca aagttaagaa tatcgccggt gtaatttttg
    19981 ataatcatca gtacaccttc gccgccatca atttgcatgg cgcattcaaa gattttatcg
    20041 ggcgtcggtg aggtgaaaat ttcgcccgga caggcccccg aaagcatccc ctgaccgata
    20101 taaccacagt gcatcggctc gtgtccgctg ccgccacccg acagcagggc gacttttcct
    20161 gcaacggggg catcagctcg ggtgacatac accggatcct gatgcagtgt cagagatgga
    20221 tgcgctttcg ccagtcctgc cagttgttcg tccagtacgt cttgcacatc attgatcaat
    20281 tttttcatta ttttgctcca gcaattacgg tagggcatgg atgatgttca acgacacggc
    20341 gaccactgac tgccgatgaa tccattgtgc atcaggcaag ggaaaagaaa attagcccaa
    20401 attatgtttc atagtgaaac atatgcttta atgaatgttc catattgaaa cttttacgtg
    20461 tattaatact taaaattgcg agccggaaca ccttttgtca taagggatgc gggatatgag
    20521 tggcgctttt aacaacgatg gtcggggcat atctccctta attgcaacct cctgggagcg
    20581 atgcaataag ctgatgaaac gggagacatg gaacgtacca catcaggccc agggcgtgac
    20641 atttgcttct atttatcggc gtaagaaagc gatgctgacg ctcgggcagg ctgcgctgga
    20701 agatgcctgg gaatatatgg caccgcgaga gtgtgcgctg tttatcctcg atgaaaccgc
    20761 ctgcattctc agccgtaatg gcgatccgca aaccttgcag cagctaagtg cacttggatt
    20821 ccatgacggc acgtattgcg ccgagggaat tattggtact tgtgcgctat cgttagcggc
    20881 tatctctggt caggccgtga aaacgatggc cgatcaacat ttcaaacagg cactctggaa
    20941 ctgggccttt tgtgcaacgc cgttgtttga cagcaagggc cgattgacgg gaacaatagc
    21001 gcttgcgtgt ccggttgagc aaactaccgc agctgatttg ccgttgacgt tggcaatcgc
    21061 ccgcgaggtc ggaaatttac tgctgacgga cagtttgctc gctgaaacta accgtcattt
    21121 aaatcaactt aatgccctgt tagaaagtat ggatgatggc gtgattagct gggacgagca
    21181 gggtaatttg caatttatta atgcccaggc ggcgcgggtc ttgcgccttg acgcgacggc
    21241 aagtcaggga cgggcaatca ctgaactctt aacgttaccc gccgtattgc aacaagcaat
    21301 aaaacaggca catccgctca aacacgtaga agcaaccttt gaaagccagc accagtttat
    21361 tgatgcggtg ataaccctta aaccgataat agaaacgcag ggaaccagct ttattttatt
    21421 gcttcatcct gtggaacaga tgcggcagtt gatgaccagt caattaggaa aagtcagcca
    21481 taccttcgct catatgccac aggacgatcc gcaaacccgc cgcttgattc attttggtcg
    21541 ccaggcggcg cgcagtagct ttcctgtcct gctttgtgga gaagagggcg tgggcaaggc
    21601 actgctaagt caggcaattc ataatgaaag cgagcgtgct gcaggtcctt atatcgccgt
    21661 caattgtgag ttatatggtg atgcggcact ggcggaagaa tttattggtg gcgatcgcac
    21721 ggacaatgaa aatggccgtc tgagtcggct ggaactggcg cacggcggca cgctgtttct
    21781 tgaaaagatt gaatatctgg cggtggagtt acagtctgct ttgcttcagg ttatcaagca
    21841 gggtgttatc acgcgactgg atgcgcggcg tttaatacca attgatgtca aagtgattgc
    21901 cacaacgacc gcggacctcg caatgctggt ggaacaaaat cgttttagtc gccagctgta
    21961 ttacgcgttg catgcatttg aaattaccat cccgcctctg cgtatgcggc gtggcagcat
    22021 tccggcgctg gtgaataaca aattacgcag tcttgaaaaa cgcttctcta cgcggctgaa
    22081 aattgatgac gatgccctcg ctcgcctggt ttcctgtgca tggcctggca acgattttga
    22141 actttacagc gtcatcgaga atcttgctct gagtagtgat aatgggcgca ttcgcgtcag
    22201 tgatttgccg gaacatctgt ttaccgagca ggcaacagat gatgtcagcg ccacacgcct
    22261 ttccaccagt ctgtcatttg cggaagttga aaaagaggca attattaacg cagcccaggt
    22321 cacaggcggt cgcattcagg aaatgtcggc tttacttggg atcggccgca ctacgctgtg
    22381 gcggaaaatg aagcaacatg gcattgatgc agggcagttt aagcgccggg tatgaaagac
    22441 agagacgatt tctgatacat cagagtgatc tatatttcat tccggcgcac gctaacattt
    22501 ttcagcatcg tttaagggct ggtctatccc gcacttaaaa gctgaagcga tatcctccgt
    22561 tgacttgctt ctgatcaaag aggttactct gcgtgtaatc cgcttcgagg tagaatgtgt
    22621 gctgtttgtt atactgtgca ctgactccca cgccgttatt ccagccatta cctttgaaac
    22681 tgtacttctc ccgggagttg ttcaacagat agtctgtatc ccctgaaaac tcgcggatag
    22741 ctccagtctt cacatacata ttcagctggc tgttgcctgc ggtgatgtca tatcccagta
    22801 tcatgctggc acgccccagc agcgattcgt agtgattcag atgtatattg aggccattac
    22861 tcgccttcat agccatctca ttctggtggc tgtatgtaag ctgggtttgc ggctctatat
    22921 agaacccata accagtaggg gacaggttga acctctgccc ggcttccagg gagatgctca
    22981 ttccattcgc agtgccgttg gcgttaacgc cgttgttctg actgtccagt acgtggaaac
    23041 tatttttctg gcgcgatgct tttataacga gatcgctgta aaaaccgttt tgtgccatgt
    23101 aactggcgta cattcccatg tagtctgaac gtgcggtacc gtcgcctccg ctatagtccg
    23161 gcgatgcatg tgttgagcca atatacagac cgacatacaa cggcattaca tcagagagac
    23221 gtttatcccc accaaactgg ataccgctgt aacccatgtc aaagccgctc agtttgccac
    23281 tggcaaagga gtccaggctt cccccataac tgcgcaacca gatattaccg tctttactct
    23341 gatttcgcag gtcacccatc cgttgcatca gcgtacggtt ttcaacatag ttcaataagt
    23401 aaccgacatt gagataattg ccgccagcat ctgcggtcgt ggtgggttta ggcgtgggag
    23461 cgggttcagg cgtaggatcg gggttgacta tgggaggctg agcgggagct ggtgtgggtt
    23521 caggattagg agtcggttcc ggaactgtcc cggaagcgta aagctcccag ttagtgccat
    23581 ttttacgcac atcgtacaga tatcccccca actcaacctg agaagacgcg ctgaacgagg
    23641 ccgcgccgtc agtggttttt accactgtca gaacttcatt tcccgttgtg gcctcgctgc
    23701 cctggttgcg gatagccaat acgtgattac cagcactact cccgctgata ttcaataaat
    23761 cccctttatt attaacgcca ttaccctcgc caacaacatc agcacgcata ataaaggtac
    23821 tgttaccgct caggttctct acgtttaatg tggcaaatgt gccggcagtt gacccgtggc
    23881 tggcaaaatc gacagttgaa tggctcagcg ccagcgtgtc gagattagaa ttacttgtta
    23941 cgttccagac gctgttattc attgcaacgt ccagttttcc gccattgaca ttatcgctga
    24001 gggaggaacc tgtccaaacc gacccagggt gcatatccag attgattagc ccacctttgg
    24061 aaagaacgct accgttgata agcatacgac ccgaggcgtt gatgcggctg gcggcataac
    24121 catcgtcatg ttgcgttgca atggccatct ggtcgggtgt actcatatca atgaccagat
    24181 cgctcgtgag gtcgatctgg ctgttggtca tggcataaat tcccctggct cctgccgcgc
    24241 cggtgatagc caaactgtct ccggttattc taccgccgct gagcgcccac aaacccagcg
    24301 ccagactgcc attacgatca actgtaatat cggtattttg catgttgaca acagccgttg
    24361 ccgtctgggc cgaggcacca taagaaccgc cggaaaagat gctgtttcgt tgcgctgccg
    24421 tgccagaaaa attgattgtc gcgtctgaac cactggtgac gaggccaccg ccctgcgcgg
    24481 aagaaatatg gctatctgca ccgatagtgg ttgtaccacc acgaacttcg acgccattgg
    24541 ccgcggctcc agttacatca acagtgagtg cattcgcgct aacctggcca aagctccaga
    24601 ggccgtgtgc attatcgcca ttggttttaa tggaactgtt tgttccgaga tcgacaacag
    24661 agtttttctg tacgtttatc cccatggcgc tgtagccctg aacatcgatt gtcaggtctg
    24721 tagccgtaaa acgcgcagca ccattggcgt tattgccgtt gagaccaccg atataaacac
    24781 ctgtacttcc atcggtcgtg attttacttc cgcttccaag atcgacactg gtgccatagt
    24841 cattgatggt taggcctata ccgttcgagt tttcaatggt gaattgagtg gctgttagtg
    24901 ttgagctatg cccaataatg atgccgtcat cgttactctt aatggtgctg cctgtgccta
    24961 agtcagcatg ggtatagtca ccaattaagt taatgccgat ggcagaggtt tgaccaacaa
    25021 catctactgt gagtcggtta gctgtcaggg agctgttttt gccctgaata atgattcctt
    25081 ttgcatatcc agaggcgtcg ttgacattga cggtgacatc attacccagg ttgatattgc
    25141 caggcgtgtt tcctgctggg gtcattacac catagaggtt tcctgatgga tcggcggtat
    25201 ctcctgtaat tctgtcacca tcattaatag taatattggt gccatattga cttcctgtat
    25261 cagcagctat cagcaggggg gaaaagataa ttgcattcaa accaatagcg ccaaatttta
    25321 tcagttgaat tgagggcgaa cggattttta attcagcgag acgatctgct ttggtattcc
    25381 cattgtgttg tttgatgccc ataccactcc tatatagtac ccagactatg ggtctatgga
    25441 ttttgtcatt ttgtttaaat caatgaataa tatcctctta tc
//
LOCUS       DAPGWD010000058        25123 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPGWD010000058 DAPGWD010000000
VERSION     DAPGWD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.058"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1221
                     /locus_tag="RHX88_002285"
     CDS             <1..1221
                     /locus_tag="RHX88_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV2230973.1"
                     /translation="PQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFL
                     SGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNIL
                     DNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKIL
                     VCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEV
                     SSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVM
                     LVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSA
                     QAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALRE
                     AKSEGGNKVIIHHI"
     gene            1402..2892
                     /locus_tag="RHX88_002286"
     CDS             1402..2892
                     /locus_tag="RHX88_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV2230974.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEKRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2935..3438
                     /gene="yeaK"
                     /locus_tag="RHX88_002287"
     CDS             2935..3438
                     /gene="yeaK"
                     /locus_tag="RHX88_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDV2230975.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3439..3543)
                     /locus_tag="RHX88_002288"
     CDS             complement(3439..3543)
                     /locus_tag="RHX88_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2230976.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3713..4159
                     /locus_tag="RHX88_002289"
     CDS             3713..4159
                     /locus_tag="RHX88_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDV2230977.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4116..4937)
                     /gene="nimR"
                     /locus_tag="RHX88_002290"
     CDS             complement(4116..4937)
                     /gene="nimR"
                     /locus_tag="RHX88_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDV2230978.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKQDPRQ"
     gene            5034..6215
                     /gene="nimT"
                     /locus_tag="RHX88_002291"
     CDS             5034..6215
                     /gene="nimT"
                     /locus_tag="RHX88_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDV2230979.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGGDKEI
                     R"
     gene            6270..6617
                     /locus_tag="RHX88_002292"
     CDS             6270..6617
                     /locus_tag="RHX88_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDV2230980.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6639..6893)
                     /gene="yoaF"
                     /locus_tag="RHX88_002293"
     CDS             complement(6639..6893)
                     /gene="yoaF"
                     /locus_tag="RHX88_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDV2230981.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7076..8101
                     /gene="dgcP"
                     /locus_tag="RHX88_002294"
     CDS             7076..8101
                     /gene="dgcP"
                     /locus_tag="RHX88_002294"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDV2230982.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8134..8232
                     /locus_tag="RHX88_002295"
     CDS             8134..8232
                     /locus_tag="RHX88_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDV2230983.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8235..8309
                     /gene="yoaJ"
                     /locus_tag="RHX88_002296"
     CDS             8235..8309
                     /gene="yoaJ"
                     /locus_tag="RHX88_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDV2230984.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8368..8616)
                     /locus_tag="RHX88_002297"
     CDS             complement(8368..8616)
                     /locus_tag="RHX88_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV2230985.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8843..10051)
                     /locus_tag="RHX88_002298"
     CDS             complement(8843..10051)
                     /locus_tag="RHX88_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2230986.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            10059..10490
                     /gene="tnpA"
                     /locus_tag="RHX88_002299"
     CDS             10059..10490
                     /gene="tnpA"
                     /locus_tag="RHX88_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDV2230987.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(10506..10694)
                     /locus_tag="RHX88_002300"
     CDS             complement(10506..10694)
                     /locus_tag="RHX88_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDV2230988.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(10698..11057)
                     /gene="yeaR"
                     /locus_tag="RHX88_002301"
     CDS             complement(10698..11057)
                     /gene="yeaR"
                     /locus_tag="RHX88_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDV2230989.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(11230..11868)
                     /gene="leuE"
                     /locus_tag="RHX88_002302"
     CDS             complement(11230..11868)
                     /gene="leuE"
                     /locus_tag="RHX88_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDV2230990.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(11995..12918)
                     /gene="dmlR"
                     /locus_tag="RHX88_002303"
     CDS             complement(11995..12918)
                     /gene="dmlR"
                     /locus_tag="RHX88_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDV2230991.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            13021..14106
                     /gene="dmlA"
                     /locus_tag="RHX88_002304"
     CDS             13021..14106
                     /gene="dmlA"
                     /locus_tag="RHX88_002304"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDV2230992.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            14357..15967
                     /gene="yeaV"
                     /locus_tag="RHX88_002305"
     CDS             14357..15967
                     /gene="yeaV"
                     /locus_tag="RHX88_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDV2230993.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            15999..17123
                     /gene="yeaW"
                     /locus_tag="RHX88_002306"
     CDS             15999..17123
                     /gene="yeaW"
                     /locus_tag="RHX88_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDV2230994.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            17179..18144
                     /gene="yeaX"
                     /locus_tag="RHX88_002307"
     CDS             17179..18144
                     /gene="yeaX"
                     /locus_tag="RHX88_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDV2230995.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLKENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(18198..19313)
                     /gene="rnd"
                     /locus_tag="RHX88_002308"
     CDS             complement(18198..19313)
                     /gene="rnd"
                     /locus_tag="RHX88_002308"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDV2230996.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(19395..21080)
                     /gene="fadD"
                     /locus_tag="RHX88_002309"
     CDS             complement(19395..21080)
                     /gene="fadD"
                     /locus_tag="RHX88_002309"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDV2230997.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(21285..21866)
                     /gene="yeaY"
                     /locus_tag="RHX88_002310"
     CDS             complement(21285..21866)
                     /gene="yeaY"
                     /locus_tag="RHX88_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDV2230998.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(21906..22601)
                     /gene="tsaB"
                     /locus_tag="RHX88_002311"
     CDS             complement(21906..22601)
                     /gene="tsaB"
                     /locus_tag="RHX88_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDV2230999.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(22659..24569)
                     /locus_tag="RHX88_002312"
     CDS             complement(22659..24569)
                     /locus_tag="RHX88_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDV2231000.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            24701..25045
                     /locus_tag="RHX88_002313"
     CDS             24701..25045
                     /locus_tag="RHX88_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV2231001.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         6115 a         6412 c         6091 g         6505 t
ORIGIN      
        1 ccacagatga attacctgat ggttgttgtg gctttatttt tccttaacgc cgtcattttt
       61 cttttcatgt taatgaaata tttcactaac aaacaaattt taccaacact cattttaagc
      121 cttgcatttt taagtggcct tatctattta gttgaaacca ttgtaattat ccataaacca
      181 attaacggca gtacactgat ccagacaaag tcgaatgatg tttctatttt ctatattttc
      241 cgccaactca gttttatttg tttaacctcg ctggcactct tttgttatgg aaaagacaac
      301 atccttgaca acaataagaa aaaaacggga atcctgttgc tggcgctgat tcctttttta
      361 gtttttcccc ttctggcaca caatctgagc agttataacg ctgactattc tttgtatgtc
      421 gtcgactact gtccggacaa ccatactgcg acctggggaa tcaactatac aaaaatattg
      481 gtttgtctgt gggcattttt actgttcttt attatcatgc gtacacgatt agccagcgaa
      541 ctatggccgt taatagcatt attatgtctg gcatcgctat gctgcaactt acttctactg
      601 actctggatg agtataatta caccatctgg tatatcagtc gcgggattga agtttccagt
      661 aaactgtttg ttgtgtcttt tctgatttat aacatttttc aggaactgca actctccagc
      721 aaactggcag ttcatgatgt gctgaccaat atttataatc ggcgctactt tttcaacagc
      781 gtagagtcat tattgtcgcg acctgttgtt aaggacttct gtgtcatgct ggttgatatt
      841 aatcagttca aacgcatcaa tgcccaatgg ggacatcgtg tgggtgataa agtgctggtt
      901 tcaattgtcg atattatcca gcaaagcatc cgccctgatg atattttagc gcgactggag
      961 ggtgaggtgt ttggcttgct atttaccgaa ctcaatagtg cccaggcaaa aatcattgcg
     1021 gaacgtatgc gtaaaaatgt cgaactcctg accggcttta gtaacagata tgatgttcct
     1081 gaacaaatga ccatcagtat tggcacggtt ttttcaacgg gtgacacgcg taatatctcg
     1141 cttgtcatga cggaagcaga taaagcctta cgcgaagcga aaagcgaggg gggcaacaaa
     1201 gtgattattc atcatattta agtgcaaaaa tattcagagc catgcttttt gcgtggcttt
     1261 tgcatacaat ttattactat tacccctaaa tttcccctcc ccagcggcgc agagatgagt
     1321 ataattagcg cccctgtgcc aggccgcaat cgaactttat ctggttttct cgtttcacta
     1381 accgaaggag tgccatttat catgaaattg caccatagaa tgctccggca ttttatcgcc
     1441 gcaagtgtca ttgtgctgac atcttccttc cttatttttg aacttgtcgc cagcgacaga
     1501 gcaatgagcg cctatctgcg ctatattgtg cagaaagcag actcctcctt tctttatgat
     1561 aagtatcaga atcagagtat tgccgcgcat gtgatgcgcg ctctcgctgc tgagcagtcg
     1621 gaagtgtcgc cagaaaagcg gcgcgccatc tgcgaggctt ttgagtctgc caataatacc
     1681 catggcttaa acctgactgc ccataaatac ccgggcttac gcggcacact acaaaccgca
     1741 tccactgact gcgacacaat tgtggaagct gcagcgctat tacccgcttt tgatcaggca
     1801 gtggaaggca accgccacca ggatgattac ggttcaggtc ttgggatggc cgaagaaaaa
     1861 tttcactatt atctcgatct caatgaccgc tatgtctatt tttatgagcc ggttaatgtt
     1921 gaatactttg cgatgaataa ctggtccttc ctgcagtcag gaagtattgg catcgatcgc
     1981 aaagatattg aaaaggtatt taccgggcgt accgtattgt cgagcattta ccaggatcag
     2041 cgtactaaac agaacgtgat gagtttgctg acgccggtat atgtcgcagg gcagctaaaa
     2101 gggattgtgc tgctggatat taacaaaaac aatctgcgga atatctttta tactcatgac
     2161 cgccctctcc tctggcgttt tctcaatgtc acgctaaccg ataccgattc ggggcgcgac
     2221 attatcatca accagagcga agataatctg ttccagtatg tcagttacgt ccatgactta
     2281 ccgggcggca ttcgtgtctc gttatccatt gatattcttt actttatcac gtcttcgtgg
     2341 aaaagcgttc tgttctggat tttgacggcg ttaattttgc tgaatatggt ccggatgcac
     2401 ttccgtttat accaaaatgt gtcgcgagaa aatattagtg atgcaatgac tggactgtat
     2461 aatcgcaaaa ttttaacccc tgaactggaa cagcggttgc agaaactggt gcaatccggt
     2521 tcttcggtga tgtttattgc tattgacatg gacaagttaa agcaaataaa tgacaccctc
     2581 ggtcatcagg agggggattt agcgattacg ttattagctc aggcgattaa acagtcgatt
     2641 cgtaaaagtg attatgccat ccgactcggt ggcgatgaat tctgcatcat tcttgtcgat
     2701 tcgacgccgc aaattgcagc acaactgcct gaacgtatcg aaaaacgtct gcaacatatc
     2761 gcgccgcaga aagagatcgg cttctcttcc ggtatttatg cgatgaaaga aaacgatacg
     2821 ttacatgatg cgtataaagc ttccgatgag cgtttatatg tcaataagca gaacaaaaac
     2881 agccgttcat gataaccttc tgtggttgtt tgcttgtaat ctcaggagcg tgaaatgact
     2941 gaaatggcta aaggaagcgt gacgcatcag cggttaatcg ctttattatc acaagaaggt
     3001 gctaacttcc gcgtcgttac ccatgaagcg gtaggtaaat gtgaagcggt gtcagaaatt
     3061 cgtggcaccg ctctaggcca gggtgctaag gcactggtat gtaaagtcaa aggcaatggc
     3121 gtaaatcaac atgttctggc gatcctcgct gccgatcaac aggccgatct gagccaactc
     3181 gccagccata taggcggatt acgcgcttcg ttggccagcc cggcggaagt cgatgaactc
     3241 acaggctgtg tcttcggcgc gatcccccct ttcagcttcc atccaaaact caaactggtt
     3301 gccgaccctc tactcttcga acgatttgat gaaatcgcct tcaatgcagg gatgctggat
     3361 aaatccgtta ttttgaaaac cacggattat ttgcgcattg cgcaaccaga actgctcaat
     3421 ttccgccgca ctgcgtaact agcctgtccg ttcaataagc agaacggaca aaaccagcac
     3481 aacagcaatg gcaaaaaacg atgacgtgat aatcagtgtc tcgacgaaca tttgatcgtt
     3541 catagcatgc ccccgggaga tagctgtttt cacgttattg ttagcgtgca caaatggcag
     3601 tttgatgaca gttcgcctga ttttttatgc aaaaaacgta aagattttct acttccttcc
     3661 tgcagcaagc gtaaagtaag caggcttatt attttttggc aaggaaacca cgatgtttga
     3721 tgtcactttg ctgatcctgc tcgggttagc tgcgctgggc tttatcagtc ataacaccac
     3781 tgtcgccgtt tcaattctgg tgttgatcat tgtccgcgtc acaccgttaa gcaccttttt
     3841 cccgtggatt gaaaaacagg ggctaagtat cgggataatc attctgacga ttggcgttat
     3901 ggcccccatc gccagcggga cgctaccgcc ctcaaccttg attcactcgt ttctgaactg
     3961 gaaatcgctg gtggcaattg cagtaggcgt tattgtctcc tggctgggtg ggcgcggcgt
     4021 gaccttaatg ggcagccagc cgcaactggt cgccgggctg ctggtaggca ctgttttagg
     4081 cgtggcgctg tttcgcggcg taccggtcgg cccgcttatt gccgcgggtc ttgtttcgct
     4141 gattgtgggg aaacagtagt taatcccgca atatatcggc ctggcgtttg ccccaggcct
     4201 ttcttaaaca tagtaataaa ggccgttgtt gaatcatacc ccagcgtatg agcaactttc
     4261 tgtacagtat cgcctttcac taatccctga agtgccataa tcagctgtaa ctgctgacgc
     4321 cactggcgaa aactcaaccc cgtctctttt actatcagtc gcgcaagatt gcgttcactc
     4381 atcgcaaaaa agccagccca ttgccctaat gacccccact cgacgggccc tttcgccatc
     4441 atctccacca tcgtgcggat tttaggatga gaagaaacgg gcaggtgcag ctgttgttgt
     4501 ggctgctgag ggagttcatc aaacaggacc tgaattaatc tgcgggtcat cggctcagca
     4561 cgctgagtcg tcgtcctgtt tgccagggtt aaaatcaatt cccggcataa gggcgatatt
     4621 ttcaatgtgc aacatgttgt cggcattgtc actgcgctgg gttcgataaa cagaaaacag
     4681 agttcagcgt tagctgtcac ttgattgcta tgttccaccc cgcccggtat ccaaaccgca
     4741 tattgcggcg gcaccatcca caaagcattt tccaccgtac aggtaattgc accatgtaat
     4801 gctagtatca gctgtccttt gcggtgctga tgtacaggac tggttaactc atccgttccg
     4861 gcgtgaatac aaaatgcgac cgcggcttca tggtgtcggt cgggttcata gccattgaga
     4921 ttcaacctgt gcatcatttt gtccgaattt agcgataatt tgtcatttta gcttgattca
     4981 acataacaat aaaaacggta aggtacagcc tcgtttgtaa caatgagaag catatgacct
     5041 gttcaacttc attaagcggc aaaaacagga ttgtccttat cgctggcatt ctgatgattg
     5101 ccacaacatt acgcgtcacc tttaccggcg cagcgccgtt actggatacg attcgttccg
     5161 cttactcgct gacgacagcg caaaccggct tattaaccac cctgccatta ttggcctttg
     5221 cgctaatctc acctttggct gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt
     5281 ttgccgcgtt acttttgatc tgtgcaggta tcgcaattcg ttctctccct tcgccttact
     5341 tattatttgg cggtacagcg gtcattggcg gtgggattgc attaggcaat gtcttactgc
     5401 caggattaat taaacgcgat ttccctcatt ccgtcgccag acttaccggc gcatattccc
     5461 tgacaatggg agctgcagcg gcactgggat cggctatggt cgtgccgctg gctttgaacg
     5521 gttttggctg gcaaggcgcg ttgttcatgc tgatgtgttt tcctctgctg gctctttttt
     5581 tatggctgcc acagtggcga agccaacaac atgcaaattt gagtacctcg cgcgccttac
     5641 atactcgggg tatctggcgt tcgccgcttg cctggcaggt cacattgttt cttgggatca
     5701 actcactggt ctattacgtg attattggct ggcttccggc gatcctcatc agtcgcggct
     5761 atagcgaagc acaggcgggt tcactgcatg gtttgctgca actagccaca gcagctcccg
     5821 gtttgctgat ccctcttttc ttacatcatg tgaaagatca gcgtggtatt gcagcgttcg
     5881 ttgccttgat gtgcgcagtg ggcgctgttg ggctctgctt tatgccagcg cacgcgatca
     5941 cctggactct gcttttcggt tttggttccg gcgcaacaat gatactgggg ttgacgttca
     6001 ttggtcttcg ggcaagttct gcgcatcagg cggcggcact ctcggggatg gcacaatccg
     6061 tcgggtattt gctggcagcc tgtgggccgc cgctgatggg taaaatacac gatgctaacg
     6121 gtgactggtc cgtaccactg atgggtgttg ccatactttc actactgatg gcgattttcg
     6181 gactttgcgc cgggggagac aaagaaattc gctaatatcc ggtgctatag tgacgtgaca
     6241 aatcatgcgt gaaagggaga acaaacacga tgaatattca gtgcaaacgc gtttatgatc
     6301 cggctgaaca gagcgatggt tatcgcgtac tggtcgaccg cctctggccg cgcggtatca
     6361 aaaaaaccga tttagccctt gatgagtggg ataaagaaat cacgccgtca acggaactgc
     6421 gcaaagcctt tcacggcgaa gtcgtcgatt ttgcaacctt tcgcgagcaa tatcttgcag
     6481 aactggcgca acacgagcaa gaaggaaagc ggctggcgga catcgccaaa aaacagccgc
     6541 tgaccctgct ctacgcagca aaaaacacca cgcagaacca tgcgctggtg ctggccgact
     6601 ggctacgtag cttgtgattt tagtacagca tccggcggtt atttttcacc agccggatgg
     6661 tcccgtcgcc acaatgccca ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg
     6721 ctgacccctt gcgctgtctg cactggaatg agcgtcccgc ccttctgctg gcaatagacc
     6781 gacgccggat ttgccatacc aatctgcggc ggtttaggtg cttctggctg agaagaggtt
     6841 gaacaaccag ccaggaccag caagcaaggc aaagcaaaac tgataatttt catttattga
     6901 tctcacacat ttatctacgc ttagagtatc gcggtatcgt tttgttttgc agcactattt
     6961 ttattacatt cactcaaaac atattacgtc ttgtttcatc tttgttgatg atgttttatc
     7021 atgcctgcaa agattaaata atcagcattt acccgccgta tcctggagtt gtttcgtgtc
     7081 agatcagatt atcgcccgcg tctcgcaatc ccttgccaaa gaacagtcac tagaaagtct
     7141 ggtccgacag cttctggaga tgctggaaat ggtcactgat atggaatcaa cctacctgac
     7201 caaagtggat gtcgaagcgc gcctgcagca tattatgttt gcccgtaaca gccagaaaat
     7261 gcacatcccg gagaatttta ccgtctcgtg ggattactcg ttatgcaaac gcgccattga
     7321 tgaaaactgc tttttcagcg atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg
     7381 caatcttggc atcaccacat ttctgagcac gccaattcac ttaccggatg gatcattcta
     7441 tggcacgctt tgcgccgcca gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt
     7501 tttacagtta ttcgctggac tgattgcaca atatattcaa aaagaggcgc tggttgaaca
     7561 gctgcgcgaa gccaatgctg cgctgattgc gcaatcgtat accgactcgt taaccgggct
     7621 accgaatcgg cgggcgattt ttgaaaatct gacgacgctg ttttctctcg cccggcatct
     7681 taaccataag ataatgatcg cgtttatcga tctggataac ttcaaattaa tcaatgatcg
     7741 ttttggtcat aatagtggcg atctgtttct cattcaggtt ggcgagcgcc ttaatacgct
     7801 ccagcaaaat ggcgaagtta ttggtcgtct cggcggtgat gagtttttgg ttgtttcact
     7861 aaacaacgag aatgcggata tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg
     7921 agaatatcac ttaggtgatg ttgatttgta ttatcccggt gccagtcttg gcatagtaga
     7981 agtcgatcct gaaacaaccg atgcagacag tgccctgcat gctgccgata ttgcgatgta
     8041 tcaggagaaa aaacacaaac agaaaacacc ttttgtcgcg catccagcgc tacattcctg
     8101 aggcgtattc acatcctttt gattggtgat aacatgcgaa tcggtattat ttttccggtt
     8161 gtaatcttta ttacagcggt cgtattttta gcatggtttt ttattggcgg ctatgctgcc
     8221 ccgggagcat aaagatgaaa aaaacaacga ttattatgat gggtgtggcg attattgtcg
     8281 ttctcggcac tgagctggga tggtggtaac gtcacctcta aaaaatagca aaggctgcct
     8341 gtgtgcagcc tttgtgcaat ttaagcgtta acttttaatc ttcctgtaga taaatagcac
     8401 gacaatcgca ccaataacgg caaccacgaa gctgccaaaa ttgaagccat cgactttacc
     8461 aaagccaaac agtgtgctga tccatccgcc gactacggca ccgactatcc ccagcagaat
     8521 agtcataaag aatccaccac catctttacc tggcatgatc cacttcgcca gaataccggc
     8581 aataagccca aaaataatcc atgacagaat gcccattgtt tcctcactta tctgttttgc
     8641 attagcgggt tagtcgctga taaaaagcat agcacaacat cgggagggca agatttgtga
     8701 cgagcatcac ggaggatttt tttgcgatgg cgcagaaatt gcgccatcaa cgatcggtga
     8761 taattaccaa ccacaaacat catgttcgtt ttccgtgtct tgacatcctc cacgccctga
     8821 atgacgagga tccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
     8881 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
     8941 aaatgttacg agcgccgttt acatcggcgt tcgctgtata tccacatacc tggcatctga
     9001 attgactttg tgacaggcga ttttctttcg ctgtatgacc acagcacgcg caacgctggc
     9061 ttgtgtacgc tggcggaaca gcaagcacct gaccgccact ccagagctgc ttgtactcaa
     9121 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
     9181 cccggacatt gcgccccggc tggctgaccg tacccgctgc tgactttgac atgttgctga
     9241 ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt
     9301 gaaggtagtc cctgcggata tttgcgatac gggaatgcag tcgctgtatt ttgcgtttct
     9361 gcttctgcca gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca
     9421 gcgtcttctg gtttttctga aaactgttta caggctcaaa gactgtgcca tctgacagcg
     9481 tggcgagttt agccacgcca gcatccagcc cgaccattga tgctgaaggg tgaaccggag
     9541 tggatacttc actttctgtc tgaatactga tgtaccactt accgcaggac tggctgaccg
     9601 tgacattttt cacaacaccc gtgacctgac ggctattccg gtagcgcatc cagcccagtt
     9661 tcggcagaaa aatacggctg ttttcctgat cgagctttac gccctgcggg tagcggaatg
     9721 catcattctg tccccgcttt ttgaatcggg gaaaagcagc ccgcttccgg aagaagtttt
     9781 tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg ctgtgagggc gaatctttaa
     9841 gccattgcgt ttcagtggcg tttttccact caaccagcca ggaagccatt ttcccgtaag
     9901 ggatgtattt attaccggcc tcatgattct cattctgacg tgccagagca cgattgaaaa
     9961 cgaaacgaca tgcgcctgcg aagcgcctca tttcacactc ctgttgacca cctggtctta
    10021 actggaattt aaatgcctgt aatcgtttca caggtcaaat gatactttgg tctgtgaaaa
    10081 aagaaaccga tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct
    10141 ttgtcaccag ataccgactc cagatttttg accatgacgc gacagaaaaa ctacgcactt
    10201 acttttcaaa tgtatgtgct gattttgaag ctgaacttgt tgaaatggat ggcgaaccag
    10261 atcacgtcca tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca
    10321 gcctcaaagg cgtatcgggt aggttactgc gacgagatcg accagatatt gcagtcaggt
    10381 attactacaa aggcgttttg tggagtcctg gctattttgc cagtagctgc ggaggtgcgc
    10441 caatatccgt catccgccaa tacattgaac aacagcaaac acctggttag gtggaaaacc
    10501 gcgccttata tccccgccct gaaggacggg gttttacggc gcaccggata agaacgcacg
    10561 gtattcacca gatcttttat cacttcagcc gccacttctg gcaccagcaa agtcatcggc
    10621 gtctctgttt cataatcgac agaaacgcca ttgctgttat tggtgacggt cacggtatac
    10681 gttgctttgc ccatgattca tttcccgtta tgaatgactt tccgttgttg cgcaccttcc
    10741 atcaggactt caggagccac gaagaagtca atgttgaaat aagtatcgtc agtcatggct
    10801 tcaatgttgt gccacttttc tggagggaac accgcaaact gccccgcttc gataaggatc
    10861 acctgatcag gctctgcact gtgttcatca gcgtagccga gatatttgac cgccccatgc
    10921 ataacggaaa ggcgtgggta aacccccggg cgcgttcctt tatcaagatg acgttcgaat
    10981 attccggcag gcgcagtttg tttattccag aaaggcgttg agcgcgtatg aatataattc
    11041 tgtgggattt gcagcatcct attccctcct tcggggaatg cgctgaaaac ggtttattcc
    11101 agccgtttca gggtacgcct gataatttgc attttaaata ccatttattg gttacttttt
    11161 agcaccatat cagagaaaaa tcagggagga ttacaagtgg gaagcccgcg acaacgcggg
    11221 ctgaatgtat caggattgca gcgtcgccag tcgggcggcg aaacccacga acatcaaacc
    11281 aatcagtgag ttgccaactt tagccagttt ctttttggta cgtatgtact gcgtgacaaa
    11341 agcaccagaa ataatcagga agctcaaata gcagaaactc accagttcca gcgtcgtcgc
    11401 cagaataaag aatgaaattc ccgtatgtgg ggcattaaca tcgataaact gtacgaaaaa
    11461 cgacacatag aacaaaatgg ctttcggatt agtcaggctc aaaattaacg cgcgtttaaa
    11521 aatggccccg tattggggct catcggattt ggcctcgtta tttttaccct tcagggtcgc
    11581 gtagagaatt ttactcccca gatagagcaa ataaaacgca ccaagataac gtacaatgtt
    11641 gaataatatc ggggtggtct taattaatgt cgccactcca gcccatgcca gaaacatcaa
    11701 tactgcatcg ccaataaata caccgcaggc cgcaagataa ccgcctttca taccgctact
    11761 gacgctattt ttgagtacaa acagggtatt tggccctggc accaacacaa taaaaatggc
    11821 cccaaccaga taggtccagt aattcagaac cccgtattca gcgaacacat taacctcttt
    11881 aattatcttt cgatcatgcg cgattaaagg tgaatatgct aaccaatctg tagcggctta
    11941 gaaaggagaa aatcaggttt taacccgata tcaacccgat aattgaatca ttaatcaggc
    12001 atgctccagt gaaaaattcg ggtagtgctc ggcaaaatac tggcgtaaaa attctaccgt
    12061 tatccgcact ttcgctgacg tcgccagcct tgaaacataa acggcccaga cgttcgctgg
    12121 ctggtaatat tccggtagca cttgcactaa atgaccactg gcaatgtttt cgctaacatc
    12181 ccaccaggag cgcagagcaa tcccctgccc atcgagacac cactgatgga caatttctcc
    12241 atgattagac gacagtgggc cggtgacttt aatcgcatgg tggccttctt tattgcgtaa
    12301 ttgccagaca ccaaaaggat gatcgcgctc tttaatgacc aggcacggca atgcggaaag
    12361 atcggttaaa tgtttcggcg cgccatgttg cgcaataaat tccggcgacg cgcagagaat
    12421 gcggtaatta gtcgccagtt tgcgggcgat taaattaggg gcgatgtcat cgccaatgcg
    12481 aatatcgaga tcaacacctt cattgaccaa atcgaccaac cggtcttcca catcaaaacg
    12541 taattccagt tgcggatacg ccttagccag cgctgataac gccggagcca ccacctgtcg
    12601 tccaaaacca aagctgctga taatacgcag catcccctgc ggcacctgac gcacgtcaga
    12661 aagttcgtcc atcatctgac cgacatcctg caaaatccgc tgcgcccatt cataaatccg
    12721 ctctccttct tcggtaatgg tgacgcggcg ggtggtgcgg tgtaacaaca ccacgtttag
    12781 cgtttgctcc agcaaggcga tgcgcttgct gacgaacgcc ggtgaaacgc ccagttcttc
    12841 cgccacggcg gcaaaaccag cccgacgagc caccagcata aagacgcgca aatcattcag
    12901 cagcggtaaa ttattcatga ttcgtgtttt atgtttcacc agttacgggg attaattcct
    12961 tttcagtcaa ttataggatg gtgatgttgt caattttgat ggtcaggaag tgagaaccca
    13021 atgatgaaaa cgatgcgtat tgctgcgatc ccgggagacg ggattggcaa agaagtcctt
    13081 cctgaaggga ttcgcgtgtt acaggctgcc gctgagcgct ggggcttcgc cttgagtttt
    13141 gagcaaatgg agtgggcgag ctgcgagtat tacagccatc acggtaaaat gatgccggac
    13201 gactggcatg agcaacttag ccgtttcgac gccatctatt ttggtgccgt cggctggccg
    13261 gacaccgttc ctgaccatat ttcgttgtgg ggttcgctgc tgaaatttcg tcgtgaattc
    13321 gaccagtacg tcaacctgcg cccggttcgt ctctttcctg gcgttccctg cccgctggcg
    13381 ggaaaacagc ctggcgacat cgatttttac gtggtcaggg aaaacaccga aggcgaatat
    13441 tcctcgctcg gcggtagagt gaatgaaggt acagagcatg aagtcgtcat tcaggaatcg
    13501 gtatttacgc gtcgtggtgt cgatcgcatt ttgcgttatg ccttcgaact tgcgcaaagc
    13561 cgcccacgta agacgctaac ttctgccact aaatcaaacg gtttagccat cagcatgccg
    13621 tactgggatg agcgagtgga agcaatggcc gagaattacc cggagatccg ctgggacaag
    13681 cagcatattg atattctctg cgcgcgtttt gtgatgcagc cggaacgctt cgatgtggtg
    13741 gtggcgtcca atttatttgg cgatatcctt tccgatcttg gcccggcctg caccggcacc
    13801 attggcattg ccccatccgc caacctgaat ccggaacgca ctttcccgtc gctcttcgag
    13861 ccagtccacg gttccgcgcc ggatatctac gggaaaaaca ttgctaaccc tatcgccacg
    13921 atttgggccg gtgcaatgat gctcgatttt ctcggcaatg gcgatgagcg tttccagcaa
    13981 gcgcataacg gtattctggc agcaattgaa gaagtgattg ctcacgggcc gaaaacacct
    14041 gatatgaaag gcaatgccac cacgccacag gttgccgacg cgatttgcaa aattattttg
    14101 cgttaaggtc aaaccagttt atttgaaccg cgtcactgac gcggtttttt tattcgttct
    14161 ttgcagtaaa taacctgcgt catttcacct tttattgttt ccgtttcgtg ttttatggct
    14221 ttccgtattc ttaattgttt aatttatgta acatgcaaat aatcaatttg ttaaacacaa
    14281 catacaactc attaccctgc aaataattac aactggcaaa agccagagac gggcttttat
    14341 gcccaggaga tgactcatga tgagcaatgt taagaaaaaa gatgtgccgc tgataagcat
    14401 cagcctggtg gccattcttt tcatcgcagc tgcattaagc cttttcccac aacaatcggc
    14461 cgacgcggcc aacgcaatat acacttttgt tacgcgtacg ttaggttccg ccgtacaggt
    14521 attggttttg ctggcaatgg gactggtgat ttatttagcc accagtaaat acggcaatat
    14581 tcgtcttggc gaaggaaaac cggaatacag cacgctctcc tggctgttta tgtttatttg
    14641 tgccggttta ggttcttcta cgctttattg gggggttgct gaatgggcct attattatca
    14701 aacgcctgga ttaaatatcg caccgcgttc acaacaggca ctcgaattta gcgtccctta
    14761 ctccttcttc cactggggca tcagcgcctg ggcaacttat acgctggcct cattaatcat
    14821 ggcttatcac tttcatgtgc gtaaaaacaa aggtctgagt ctttccggca ttattgccgc
    14881 gattaccggc gttcgcccgc aaggcccatg gggaaaactg gttgatttga tgttcctgat
    14941 cgccactgtc ggcgcactga ctatttccct tgttgtcacc gcagcaacct ttactcgtgg
    15001 actttccgcg ctgaccggtt tacccgataa cttcaccgtg caggcatttg tgatcctgct
    15061 ttccggcggc attttttgcc taagctcgtg gattggtatc aacaacggtt tgcaacgtct
    15121 gagcaaaatg gttggctggg gcgcgttcct gctgccatta ctggtgctga ttgtcggccc
    15181 aaccgaattt attaccaaca gcatcatcaa tgccatcggt ctgaccacgc aaaacttcct
    15241 gcaaatgagc ttattcaccg atccgcttgg cgatggttca tttacccgca actggaccgt
    15301 tttctactgg ctgtggtgga tctcatacac ccctggcgta gcaatgtttg tcacccgcgt
    15361 ttcccgcggt cgtaagatta aagaagttat ctggggactg atcctcggca gcaccgtcgg
    15421 ttgctggttc ttcttcggcg taatggaaag ctatgccatt catcagttta tcaatggcgt
    15481 aatcaacgtc ccacaggtgc tggaaacact gggcggcgaa acagctgtac agcaagttct
    15541 gatgtcgttg ccagccggta aattgttcct cgccgcatac ctgggcgtga tgattatttt
    15601 ccttgcctcg catatggatg cggtggccta caccatggct gcgaccagta cgcgtaatct
    15661 ccaggaaggt gacgatcctg accgtgggct gcgtcttttc tggtgcgtgg tgatcactct
    15721 gatcccgctt tccatcttgt ttaccggtgc ttcgctggaa acgatgaaaa ccaccgtcgt
    15781 gctcacagcc cttcccttcc tcgtcatttt actggtgaaa gtcggcggat ttattcgctg
    15841 gctgaaacag gattacgccg acattccggc tcatcaagtt gaacattatc tcccgcagac
    15901 accggttgaa gccctggaaa aaacgccagt gctccctgcg ggaaccgtat tcaaaggcga
    15961 caactgagcg ccatcatcct aacgataaag gtatccctat gagcaatctg agccctgact
    16021 ttgtactacc cgaaaatttt tgcgctaacc cgcaagaggc gtggaccatt cctgcccgtt
    16081 tttataccga tcagaacgcg tttgaacacg aaaaagagaa cgtcttcgcc aaaagctgga
    16141 tttgcgtcgc tcacagcagc gaactggcga atgccaatga ttatgtgacg cgtgagatca
    16201 ttggcgaaag catcgtgctg gtacgcggtc gtgataaggt tttgcgcgcg ttctataacg
    16261 tgtgtccgca ccgtggtcat cagttgttga gcggtgaagg aaaagcaaaa aatgtgatta
    16321 cctgcccgta tcacgcatgg gcattcaaac tcgatggcaa cctggcccat gcacgtaact
    16381 gcgaaaacgt cgccaatttc gatagcgaca aagcgcaact ggttccggtg cgtctggaag
    16441 aatatgccgg attcgtcttc atcaacatgg accccaacgc caccagcgta gaagatcaat
    16501 tacccggcct gggcgcgaaa gtgctggaag cctgcccgga agtccacgat ctgaaactgg
    16561 cggcccgctt taccacccgc acgcctgcca actggaagaa cattgtcgat aactatctcg
    16621 agtgctatca ctgtggtccg gcgcatccag gtttctccga ctccgtacag gttgatcgtt
    16681 actggcacac catgcacggt aactggacgc tgcaatacgg tttcgccaaa ccgtccgaac
    16741 agtcgtttaa atttgaagag ggtacggatg cggcattcca cggtttctgg ctgtggccgt
    16801 gcacgatgct gaacgtcacc ccgatcaaag ggatgatgac ggtcatttat gaattcccgg
    16861 tggattctga aactaccctg caaaactacg atatttactt caccaatgaa gagttaaccg
    16921 acgagcaaaa atcgctgatt gagtggtatc gcgatgtgtt ccgtccggaa gatttacgtc
    16981 tggttgaaag cgtacagaaa gggctgaaat cgcgtggcta tcgtggtcag gggcgcatca
    17041 tggccgacag tagcggtagt ggcatttccg aacatggtat cgcccatttc cataatctgc
    17101 tggcgcaggt gtttaaggac taatgacatc ggcggcggta ttttccgccg ctgggctgat
    17161 ttttgatgga gtacagcaat gtcagactat caaatgtttg aagtacaggt gagccaggtt
    17221 gaacccctta ccgaacaggt gaaacgcttc acgctggtgg caaccgatgg caaaccatta
    17281 cctgcgttta ctggaggaag tcacgtcatt gtgcagatga gcgatggtga taaccagtac
    17341 agcaatgcgt attcactact gagttcgccg catgacacct cttgttatca gattgccgtt
    17401 cggctgaagg aaaactcgcg cggcggttcc cgctttttgc atcagcaggt aaaagtgggc
    17461 aatcggttaa cgatttcaac gcctaataac ctgtttgcgc taattccctc agccagaaag
    17521 catctgttta tcgcgggcgg tattggtatc acccctttcc tgtcgcacat ggcagagctg
    17581 caacacagcg acgtcgactg gcagctacat tactgctcgc gaaatccaga aagttgcgca
    17641 tttcgtgatg agctagtcca gcatccgcag gctgagaaag ttcacttgca tcactcatca
    17701 accggaacac gactggaatt agcgcgatta ttggcggata tcgaacctgg cacacacgtt
    17761 tatacctgtg gtcccgaggc gctaaatgaa gcggtaagaa gtgaagctgc gcgtctggac
    17821 atcgtcgccg atacgctgca ctttgagcaa tttgctatcg aagacaaaac cggcgatgcc
    17881 tttaccctgg tgcttgcccg ttccggaaaa gagtttgtgg tgccggaaga gatgactatt
    17941 ttgcaggtta ttgaaaataa taaagccgcg aaagtggaat gtttatgtcg tgaaggggta
    18001 tgcggaacct gcgaaacagc aatactggaa ggtgaagctg accatcggga tcagtatttt
    18061 agcgatgaag agcgtgccag ccagcaaagt atgttgatct gttgttcgcg tgcgaagggt
    18121 aaacgcctgg tgttggattt gtagtttgcc aaagccggat gtggcgctga gcgcgcccag
    18181 tccggcttcg gaagatttta ctgcggatat tcctgcaata aattgtgtaa tgcttccgcc
    18241 atcagctcac cacgccagcc ggaaatcagc tccggcaaat tgttctgcgg tttcagtttc
    18301 cagtgccagt tcagcagttg gttgatttgc cgacgcgatg ccagcaattc ggcgctgatc
    18361 ttatgcgttt cgctcacgtc agtaatcagc gacttaatcg ctttaaacgc tttacgataa
    18421 ccaggcatat ccatcaggtt aagcatcggc tgcggtaagg cctcttccgg caatgtctgc
    18481 gctttttcca ccagcgccag cagcgtttta ccgtgaaagc ggatttcgct accggataaa
    18541 cccaggctgt ccagttcgcc taaacttccc ggcatataac gcgcgaccga ccacaaatgc
    18601 tcttcacgca cgacaaagtt caccgccaga tcgcgctctc gcgccttgcg cagtcgccag
    18661 tcggctaaca gttgcagaca ggccagttgg cgtgtgcgta attgccaggc attggtgata
    18721 tcacgccagg catcttccgg cgcaacgact tcctgacgac gcatttgcat caggcggcat
    18781 tcatccagcg ccgcaggtag ccagccggag gcctccgttt ctaccataag cttggcggtg
    18841 atcggtaaca gataccagac atccgccgct gcgtattcac actgacgttc ggtcagcggt
    18901 ctggccagcc agtcggtgcg cgattcactc ttgtccagcg taacgccgga atactcttcc
    18961 accatggaag cgaaacccca tgacatcggg cgtccgcaga aggcagcaag gatttgcgtg
    19021 tcaatcaagg gttgtggtaa ttcgccaaag acattgagga acacttccag atcttcactg
    19081 cctgcatgga gaaattttgt gatggacgga tcgcgcagga tcgctttcag cggtgaccag
    19141 tcggtgatcc cgagaggatc aattagcgcc agatgctcgc catcgaaaag ttgaatcaac
    19201 cccagctgcg ggtaataagt gcgcgtacga acaaattcag tatccagggc tatcgccgga
    19261 aaggcacgga cggcttcaca caaagaagcc agcgcatcgt ccgtggtaat catttggtaa
    19321 ttcaaattgt tttctcttta gtgggcgtca aaaaaaacgc cggattaacc ggcgtctgac
    19381 gactgactta acactcaggc tttattgtcc actttgccgc gcgcttcgtc acgtaattct
    19441 cgtcgcaaaa tttttccgac gttagatttc ggtaactcat cacgaaactc caccagcttc
    19501 ggtactttgt agcccgtgag ctgacggcgg caaaaggtca ccagtgactc ttcggtaagc
    19561 gatggatctt ttttcactac gaagattttc accgcttcac cactggagcc ggaaggtacg
    19621 ccaacagccg cgacttcctg tacgccagga tgctgcatga cgacatcttc aatctcgttg
    19681 ggatagacgt taaaaccgga aaccagaatc atgtcttttt tacgatcaac aatgcgcagg
    19741 aatccttctt catccattac cgcgatgtcg ccggtgtgta accagccatt tttgatgatt
    19801 tcatcggtag catcgggacg ctgccagtaa cccagcatca cctgcggtcc tttgacacaa
    19861 agctcacccg gttgacctgg tggtacttca ttatcatcat catccaccag tttggcttcc
    19921 gtcgacggca ccggcaaacc gatgctacca ctatgataat caatatcata tgggttaacg
    19981 ctgaccagcg gcgcacactc ggtaaggcca tagccttcca gcagatactg tccggtcagt
    20041 ttcacccaac gctctgccac cacttgctgc actggcatcc ctccgcctgc ggaaagatgc
    20101 agactggaga aatccagctg ctggaactct ttattgttca gcaacgcatt gaacaaggtg
    20161 ttaacgcccg tgatagcggt aaacggatat ttcgctaact cttttaccaa ccctggaata
    20221 tcgcgcgggt tagtgataag caggttctgc ccacccagtt cgataaacag caggcagtta
    20281 atggtcaggg caaaaatgtg atacagcggc agtgccgtta ctaccagttc tttacctgga
    20341 tgcagcaacg gcccataggt ggcgttgacc tgttccaggt tcgccagcat attgcggtga
    20401 gtcagcatcg cgcctttcgc cacacctgtg gtgccgccgg tgtattgcag gaaggctaaa
    20461 tcttccggca ccagttcggg tttgacgtac tgcatccggt agccgttatg cagtgcgcta
    20521 cgaaatgaaa tggcatctgg cagatggtat ttcggcacca aacgcttgat gtatttaaca
    20581 acgaaattga ctaccgtgcc ttttgccgta gatagctgat cgcccatacg ggtcagaatt
    20641 acgtgctgaa cggcggtttt atcaaccact ttttccagtg tgtgagcaaa gttagacacg
    20701 ataacaatcg ccgatgcgcc gctatcgtta agctgatgct caagctcacg cggggtatac
    20761 aacgggttaa cgtttacgac gatcatcccg gcacgcaaaa tgccaaacag cgccaccgga
    20821 tattgcaata aattaggcat catcaacgca acgcgatcgc ctttcttcag ccccaaccct
    20881 tgttgcaaat aagcggcaaa cgcgcgactg cgttcttcca gcttgcggaa ggtcattacc
    20941 tcccccatat tcacaaacgc aggctgatcg gcgtagcgcg cgaccgactg ctcaaacata
    21001 tctaccagag attgataacg gtcagggttg atctccgtcg gaacgtccgc gggataacgg
    21061 ttaagccaaa ccttcttcaa ttcttcacct ctaaaatgcg tgttcgtcgt catcgcaacc
    21121 ccaaatgata tacatgccgt taacataata ttaactcatc ataccagctt gataattacc
    21181 caacgaaaag gttgcgaagc gcgtcactat ttatttttat ctttaccgta agaatgcaga
    21241 aacagcggac cagccgctgt ttctttttct ttaaaaacaa gcaattattc agttacaact
    21301 gtttgtactc tcgcggggcc gggattatac cagccccatc cgccgtagcc atagggccag
    21361 ccacgaccgc cataaaacca tggatcaatc ggctgaggcg gcataatcac ctgctgggtt
    21421 aaatgccaac gtttgtaacc cgttacttgc atcaccataa atttataggg cgtgttgccg
    21481 atttttccgt caaccgcacc agtgattggc ccgactaccg taaccagttg tccacggaaa
    21541 tccaccgggt ccagaaaacc gttcacatcg gcataaatgc gaccgcgaga aggttctccc
    21601 agcgtcggtc tggcaccgct gtccagcggt acggtagcaa tttccaggcg ggttttccct
    21661 tgctggtttt gtaccgcaac cactttgcca ccaaagcgtg cctcctgacc aacgtacagc
    21721 tgcggcgcac tcatcacccg aactaaatct tgttgcggcg tgggactgct gcctttaatg
    21781 gcgtccggca cagtgacaca accgctcagc attagcgcaa acgtacctgc cagtatgcct
    21841 ttgataacat tcttttgaac cgccatgatg cgactccttt ttttctcagg gcatactctt
    21901 aagattcatt ctttgcccgg aagtttcttc catgcgacgt tgttacgtaa ataaaccggt
    21961 tcggcatgtt ccaccgccac cgttttaccc tcggcaaaca tctgacaagc aatcggcagc
    22021 atatcttctg cagcggggag taacacttcg ccatcgcgca aaaccagtcc gctctcttta
    22081 ccgagatccg gccaggcttg ccagcctgta cctaccgtca cccattcccc ggaaagctgt
    22141 tgcattcgtt catgaacgag ttcgggtttg agtacggctt cggtttcttc accgtgccag
    22201 ataccgtttt catcacgctg atattcggcc cagtaaactt cgcccattcg cgcgtcaatg
    22261 gctgacagca cgcgtgttgc gccgtttttg cgccacgccc cttgcgccat cgtcattagc
    22321 gtggagacgc caatcatcgg taattccgcg ccaagcgcca gcccttgcgc gatgccaata
    22381 ccaatgcgca cgcctgtaaa gctaccgggg ccgcgcccgt aagccagagc gttaatatca
    22441 gtcagggaag ttccgctggt ggtcaggata tcctgcacca tcggtaagat tcgttgagta
    22501 tgttcacgag ggcaaagctc aaaatgagcg ttgacagtac cgtcgttcca cagggcgaca
    22561 gagcaggcct ctgtcgcggt atcgatagcc agaattcgca tgggtcttcg tgcttagatc
    22621 aataaaaagg cgcgcatcat accatactcc gtaacaaatt acctggagga tggtatcgca
    22681 aggaaacgaa ccgcacgggc aatgtcacgg gtgcgtggcg cgggcggcag actggcgaga
    22741 aacgtcgcgc cgtaaggacg catcaccagc cgattgtcac aaattaccaa aaccccgcga
    22801 tcgtcggcgt cgcgaatcag tcgcccgact ccctgcttga gagtaatcac cgcatccggc
    22861 agttgtactt catcgaacgg gtcgccaccg cgcaaacggc aatcttccat gcgcgctttt
    22921 aacagtggat catcaggcga ggtaaacggc aatttgtcga taatcaccaa tgacaatgta
    22981 tcgccacgca cgtccacccc ctcccagaaa ctactggttg ccacaagaag cgcgttaccg
    23041 gcactgacaa attgctgcaa cagttgccct ttgctggttt ccccctgcaa taatacggga
    23101 agcgtcatgg tagcgcggaa ctgctcggcc agatcgcgca tcatggcgtg cgaggtacaa
    23161 agcataaaac agcgaccgtt gttagcttcg atgatcgggc gcagcatagc cgccagttgc
    23221 cgcgcggaac ccggttgatt ggtttgcggc aggttgcgcg gcacacagag taacgcctgg
    23281 cggctgtaat caaacgggct tggcaatagc aacgactccg cctgttctat cccaagccgc
    23341 gaggtgaaat gatgcagatc gtcgttcacc gacagcgttg ctgaggtgaa gatccagcta
    23401 ccgggctttt gcgccattaa ctctttgaat ttatccgcca cgctgagcgg cgtgagagcc
    23461 agagtaaaat ggcgcgaagt gcattcgtac cagtagctgt agcccggctg attgatctct
    23521 tttagccgct tcagccgtgt gcgatacaac gtggcgcgct caaatgccgc atccagcaag
    23581 gcggaacgcc ccagtgacag tttcgccacg tcataacaaa gttccagggt gtcatcgagc
    23641 agtaaaaatg cccgctgaat ttgcggatta gctaacagct cacgcaggtt accgcgataa
    23701 cctggttcac cgagttgcag acgaaaatcc tgcgcactct gggcaagacg atcagcgcac
    23761 ttttgtaact gctgggtgtc ttttaattcg gtgcggtaag cgatggtgat gtcttttgcc
    23821 aggtcgagca gttgtcgact ggagagtgac tgaccaaaat actggctggc aatgtccggt
    23881 agctggtggg cttcgtcgaa gatcatgacg tccgcttccg ggatcaactc gccaaatcca
    23941 ctctctttaa ccaccatatc cgccagaaag agatgatggt ttaccaccac cacatcggcg
    24001 tccatcgctt ttttacgtgc tttgaccaca aagcaatctt tatacatcgg gcagtcgctg
    24061 ccaagacagt tgtcgttggt gctggtaacc agcggccacg cctgtgaatc ttccgccacg
    24121 ctgacgcagg tgctgatatc accatcgact gtttgattag accaggagcg cagcaggatc
    24181 acatcgctta agatttgtac cggcagatcg ccccccgcca gcgcctgctg ttcgagacgt
    24241 tcgaggcaga ggtagtttga gcgccctttc agcagcgcca cgttgcccgt atatttcaat
    24301 gcctttgaga ctgttggcaa atcgcggctg tagagctgat cctgcaacgc ttttgagccg
    24361 gtcgagataa tgactttctt tttcgcccgc agcgcaggag ccaggtaagc gtaggttttg
    24421 cccgtaccgg ttcctgcttc caccaccagc ggctggcctt tttctatcgc ctgggtgacg
    24481 gctaccgcca tctgtcgctg tggttctcgc ggcttaaagc ctggtatcgc tttcgccagc
    24541 tgaccgtctg gtgcaaaatc gtccgtcaca ctaccccctg ttgatttgaa cagggattat
    24601 gtcaggatga gggcgctttc gccagttgaa gtggtgacgg cgacctcaca ttgtggcagt
    24661 ctttgcagca cgaaaatgga aggataatga ggacaaaatg atgactatcg ttcgtatcga
    24721 tgctgaagcc cgctggtctg atgtggtaat ccacaacaac acgctctact acactggtgt
    24781 accggaaaac ctcgacgccg atgcctttga gcaaaccgcc aacacgctgg cgcagattga
    24841 tgccgtgctg gaaaaacagg gcagcaataa atcgagcatt ctggatgcca ccattttcct
    24901 ggccgataaa aacgacttcg cggcgatgaa taaagcgtgg gatgcctggg ttgtcgcggg
    24961 tcatgcgccg gtgcgctgca cggtacaagc gggtttgatg aacccgaagt ataaagttga
    25021 aattaagatt gtggctgcgg tgtaagcttt atcgaagcaa aataagtcag acgataattt
    25081 atcgataata ctggtcggtt ttacataaat cgaccagaga tta
//
LOCUS       DAPGWD010000059        24770 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPGWD010000059 DAPGWD010000000
VERSION     DAPGWD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.059"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            117..1553
                     /gene="mdtQ"
                     /locus_tag="RHX88_002314"
     CDS             117..1553
                     /gene="mdtQ"
                     /locus_tag="RHX88_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDV2231002.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1606..2367
                     /locus_tag="RHX88_002315"
     CDS             1606..2367
                     /locus_tag="RHX88_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2231003.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2497..3075)
                     /locus_tag="RHX88_002316"
     CDS             complement(2497..3075)
                     /locus_tag="RHX88_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2231004.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            3245..3832
                     /locus_tag="RHX88_002317"
     CDS             3245..3832
                     /locus_tag="RHX88_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDV2231005.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4006..4938
                     /gene="pbpG"
                     /locus_tag="RHX88_002318"
     CDS             4006..4938
                     /gene="pbpG"
                     /locus_tag="RHX88_002318"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDV2231006.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(4976..6691)
                     /gene="dld"
                     /locus_tag="RHX88_002319"
     CDS             complement(4976..6691)
                     /gene="dld"
                     /locus_tag="RHX88_002319"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV2231007.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            6887..9184
                     /gene="bglX"
                     /locus_tag="RHX88_002320"
     CDS             6887..9184
                     /gene="bglX"
                     /locus_tag="RHX88_002320"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDV2231008.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9395..10312
                     /gene="osmF"
                     /locus_tag="RHX88_002321"
     CDS             9395..10312
                     /gene="osmF"
                     /locus_tag="RHX88_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDV2231009.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKATAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10319..11476
                     /gene="yehY"
                     /locus_tag="RHX88_002322"
     CDS             10319..11476
                     /gene="yehY"
                     /locus_tag="RHX88_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDV2231010.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11469..12395
                     /gene="yehX"
                     /locus_tag="RHX88_002323"
     CDS             11469..12395
                     /gene="yehX"
                     /locus_tag="RHX88_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDV2231011.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFHHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            12400..13131
                     /gene="yehW"
                     /locus_tag="RHX88_002324"
     CDS             12400..13131
                     /gene="yehW"
                     /locus_tag="RHX88_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDV2231012.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13112..13219)
                     /locus_tag="RHX88_002325"
     CDS             complement(13112..13219)
                     /locus_tag="RHX88_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDV2231013.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(13279..14010)
                     /gene="mlrA"
                     /locus_tag="RHX88_002326"
     CDS             complement(13279..14010)
                     /gene="mlrA"
                     /locus_tag="RHX88_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDV2231014.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14232..15917
                     /gene="btsS"
                     /locus_tag="RHX88_002327"
     CDS             14232..15917
                     /gene="btsS"
                     /locus_tag="RHX88_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDV2231015.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            15914..16633
                     /gene="yehT"
                     /locus_tag="RHX88_002328"
     CDS             15914..16633
                     /gene="yehT"
                     /locus_tag="RHX88_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDV2231016.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16680..17150
                     /locus_tag="RHX88_002329"
     CDS             16680..17150
                     /locus_tag="RHX88_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDV2231017.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(17190..17651)
                     /locus_tag="RHX88_002330"
     CDS             complement(17190..17651)
                     /locus_tag="RHX88_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDV2231018.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(17776..19776)
                     /locus_tag="RHX88_002331"
     CDS             complement(17776..19776)
                     /locus_tag="RHX88_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDV2231019.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCTTAQPTEKEE
                     AWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(19773..20909)
                     /locus_tag="RHX88_002332"
     CDS             complement(19773..20909)
                     /locus_tag="RHX88_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV2231020.1"
                     /translation="MSKLNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFVFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(20902..23181)
                     /locus_tag="RHX88_002333"
     CDS             complement(20902..23181)
                     /locus_tag="RHX88_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231021.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRLREAFMARWIGWAMQQNNGDVLVVCGGWHAPVLAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAVLGAL
                     MRLEHPNTSAEAALTMLAQLSPAQSGEALQGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(23192..24280)
                     /locus_tag="RHX88_002334"
     CDS             complement(23192..24280)
                     /locus_tag="RHX88_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDV2231022.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRHFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5765 a         6314 c         6773 g         5918 t
ORIGIN      
        1 tatatttttc tggatatgtc gttttattgc tactgatgta ttgccacgcg aaactaaaaa
       61 atatatcgca acacacggta atctccgcgc accttttctt acctgattat tcccccatga
      121 atcgtgattc cttttatccc gccatcgcct gttttccgct gttactgatg ctggccgggt
      181 gtgcgcctat gcatgaaacc cgccaggcgt taagccagca aacgcccgct gcacaagttg
      241 acaccgcatt acccacggcg ctgaaaaatg gttggccaga cagccaatgg tggctggagt
      301 atcacgataa tcaactcacg tccttaatta acaatgcact acagaacgca ccggatatgc
      361 aggtggctga acagcgcatt cagctagctg aagcccaggc gaaggcaatt gctactcagg
      421 atggtccgca gatcgacttt tctgcggata tggagcggca aaaaatgtcg gcagaaggct
      481 taatggggcc gtttgctctg aacgatccgg ccgcaggtac gaccggtccg tggtacacca
      541 acggaacttt tggcttaacg gcgggctggc atctcgatat ctggggaaag aatcgggcgg
      601 aggttactgc ccgcctgggt acggttaaag cacgggcggc ggaacgcgag caaacccgcc
      661 aattgctggc tggcagcgta gcccgcctgt actgggagtg gcaaacccag gcggcgttaa
      721 acacggtctt gcagcaaata gaaaaagagc agaacaccat tatcgcgacc gatcgccagc
      781 tatatcagaa cgggattact tcttcagttg aaggtgtgga aaccgatatt aatgccagca
      841 aaacccggca gcagctcaac gatgtcgcgg ggaaaatgaa aattattgag gcacggttaa
      901 gcgcacttac aaataaccag acaaagtcat tgaagcttaa accggtcgcg ttgccgaaag
      961 tggcaagcca gcttcctgat gaactggggt actccttatt ggcccggcgg gcagatttgc
     1021 aggcggcgca ctggtacgtt gagtcatcgc taagcaccat tgatgcggca aaagcggcat
     1081 tttatcctga catcaacctg atggccttcc tgcaacagga tgcgttgcac ttaagcgatc
     1141 tgttccgtca ttccgcgcag caaatgggcg ttacggcagg cctgacgcta cccattttcg
     1201 atagtggtcg tcttaacgcc aatctcgata tcgcaaaagc cgaaagcaac ttgtctatcg
     1261 ccagctacaa caaagcggtg gttgaagcgg tgaatgacgt ggcgcgggca gccagtcagg
     1321 ttcagacact tgcggagaaa aaccagcatc aggcgcaaat tgagcgcgat gccttgcgtg
     1381 tggtaggtct tgcgcaggcg cgctttaacg cgggcatcat tgctggttcc cgcgtcagcg
     1441 aagccagaat ccccgcgctg cgtgagcggg ccaatggcct gttattgcaa gggcagtggc
     1501 tggatgcttc cattcaactc actggtgcgt tgggcggggg ttacaaacgc tgatgatatg
     1561 cgccttctat acttaacgtt tattcagcgt taagtggaga actcgatggc acaggttgcg
     1621 attattaccg cctccgattc ggggatcggc aaagagtgcg cgttattact ggcgcagcag
     1681 gggtttgata ttggcattac ctggcactca gatgaagaag gggcaaaaga taccgcgcgt
     1741 gaggtagtta gccacggcgt acgtgcggag attgtgcagc tggatctcgg taaactgcca
     1801 gaaggtgcac aggcgctgga gaaactcatt caacggctgg ggcgtattga tgtgctggtg
     1861 aataatgcgg gcgcaatgac caaagcgccg tttcttgata tggctttcga tgagtggcgc
     1921 aagattttta ccgttgatgt cgatggtgca tttttatgct cacaaattgc ggcccgccag
     1981 atggtgaaac aagggcaggg tggtcgcatc atcaacatta cgtcggtaca tgaacatacg
     2041 ccgctgccgg atgccagcgc ctacacagcc gctaaacatg cgctcggcgg gttaaccaaa
     2101 gcaatggcgc tggagttggt caggcataag attttggtga acgcagtcgc acctggggcg
     2161 atcgctacgc caatgaacgg catggatgat agcgacgtga agcccgacgc ggagccttcg
     2221 attcctctgc ggcgttttgg cgcaacgtat gagattgcca gcctggtggc gtggctttgt
     2281 tcggagggcg caaattacac caccgggcag tcgttgatag tggatggcgg ctttatgttg
     2341 gcgaatccgc agttcaaccc agaatagcgc agtgaggtgt gtggatattt caatgggtaa
     2401 aattttggta ggcatgataa gacgcacaag cgccgcatca ggcatggtgc accggatgtc
     2461 ggatgcggcg cgaacgcctt atccagggca atggttttac gcctgattat ccggcttttt
     2521 cttcccgcgt cgtttcagcc accagcgcac gcctaccacc agaaccacga ccagaatcaa
     2581 ccagacccag tgcttcaaat gctgatcgag attgtgcaac cacggcgcaa tcacctgacc
     2641 accagcgtaa ccgatagtgg taaaaatcaa cgcccaggca aatgcgccga gaatattaag
     2701 cggcagaaag attttcggcg gcagctggct ggcaccaatc agcgtcgggc caatcacccg
     2761 aaagccatac ataaagcgcg taccaatgac aaacagatac ggatggcgtt ggataagttt
     2821 ctgcgcccgc tcaattttat cctgatgttt cgagaaacgg cgtaacagct tgccgccaaa
     2881 ccgccgcccg cacagataaa gcacctggtc gccaatcatg ccgccaagcg ccacagaaag
     2941 taccaccagc gggaacttta atagcccctg atgcgccgca acgcctccca gcaaagtcac
     3001 ggtttcacct tccgccaggc taccgatcac cagcgcggca taaccatatt gtgagatgag
     3061 tgtattgaga tccataggcc agtctgtctc cctttggttg cctgtaagca tacaccctgg
     3121 aaatcattca cgctgaaaaa ggacgtacat cagacggtgc aaattacatg ggcgaacaaa
     3181 aaataatcac aagctgactt atacttgaac tgtcaggttc atgaactcac aacaaggggg
     3241 cggtatgagc catgtctggg gactgttttc ccatcccgat cgtgaaatgc aggtgattaa
     3301 tcgcgaaaac gaaacgattt ctcaccatta cacccaccac gttttgctga tggcggcgat
     3361 tccggtgatt tgcgccttca ttggcactac acagattggc tggaattttg gcgatggcac
     3421 tatcctgaag ttatcctggt ttaccggact ggcgctggct gtcttgtttt acggcgttat
     3481 gctggctggg gtcgcggtga tggggcgggt catctggtgg atggcgcgta attatccgca
     3541 gcgtccgtca cttgcgcact gcatggtctt cgcgggctac gttgcaactc cgctgttttt
     3601 aagcggtctg gtggcgcttt atccactggt ctggttgtgt gcgctggtcg gcacggtggc
     3661 gctgttttac accggctacc tgctttacct tggtattccg agcttcctga atatcaacaa
     3721 agaggaaggc cttagcttct ctagttcgac gctggcgatt ggcgtgctgg tactggaagt
     3781 gctgctcgcc ctaaccgtta tactatgggg ctacggttat cgtttgttct agaaagttct
     3841 ttgacgttgc attgctggcg taaatgtcag caatgcagca tttcctcacg attctccttt
     3901 gacgatcgca ctttttgctc gttatgatag cgtcgctagc ctcagtaaat ccttactccg
     3961 gcggtgcgca acccgtgcgc gtgaaccact atctgaatgc tcatcatgcc gaaatttcga
     4021 gtttctttat ttagcctggc cctgatgctg gctgtgcctt ttgcaccgca ggccgttgcg
     4081 aaaacggcag ccgctaccac cgcttcacaa ccggaaattg cctccggtag cgcgatgatt
     4141 gttgatctga ataccaacaa agtgatctat tcgaaccacc cggatctggt gcgtccgatt
     4201 gcgtctatca gcaaattaat gaccgcgatg gttgtgctgg atgcacgact gccgctggat
     4261 gaaaaactaa aagtggatat cagccagacg ccggagatga aaggggtcta ttcgcgcgta
     4321 cgactgaata gcgaaatcag ccgtaaagat atgctgttgc tggcgctgat gtcttcagaa
     4381 aaccgcgcgg cggcaagcct tgcacaccat tatcccggtg gttacaaagc ctttattaag
     4441 gcaatgaatg cgaaagcgaa atcgctcgga atgaacaaca cgcgctttgt tgaacctacc
     4501 ggattgtcgg tgcataacgt ttcaactgcc cgtgacttaa ccaaactgct cattgccagc
     4561 aaacaatatc cgttgatcgg gcagttaagt actacccggg aagatatggc aaccttctct
     4621 aacccgacgt atacgctgcc gttccgcaat actaatcatc tggtgtatcg cgataactgg
     4681 aatattcagt taaccaaaac cggttttacc aatgcggcgg gccattgtct ggtgatgcgt
     4741 acggttatca ataataaacc ggtggcgctg gtagtgatgg acgcgtttgg caaatatacc
     4801 cattttgccg atgccagccg cctgcgtacc tggattgaaa ccggtaaagt gatgcctgtg
     4861 ccggcagcag cgttgagcta taaaaaacaa aaagccgccc aaatggcggc ggcggggcag
     4921 acggcacaga acgattaagt gaattcggat ggcgatactc tgccatccgt aatttttact
     4981 ccacttcctg ccagtttttc cgtttactgg ttttaccgat ccccggattc atgctgttgg
     5041 tcggatcgtt ctcgcgatag aacttctgca acgtctccgg tgctttatac aaatgaccga
     5101 cgttatgctc ggcagggtat tgcgcgccgc gctgctgtag cagttccagc atctgttctt
     5161 ttaacgcatg cacatccacg ccttttttca ctatgtaatc ctgatggaag acatagcaca
     5221 taaaatggcc gtaatagagc ttgtgcacca gctggctgtc gatctccggc ggtaaatgct
     5281 cataccactc ggtgtcgtta cgccgcagag cgatatccaa cgccagaatg tcttcgactt
     5341 catcggaatg caccgcctga taacgaattg ctgcgcccgc agcggcgaaa cggtgtaaaa
     5401 acgctttgct gccttcctcc ggcgtacaga caaagaaatc gccttcggcc tgtttgaaat
     5461 aatccaccaa ccacgatttg gcttcgccca cgccatcgcc cgccattttt aacagcagat
     5521 gatgctcgta tttatcgcgc cagtttttca tgcgcggcgg taaatggctg gggaacaggt
     5581 gaccgaattt ttgcatcgca cggtcagtaa aatgcggacg gaagaatttc actttctcca
     5641 gcatcgcatc ggtgcgtccc ttgagattaa agaagaacgg catcttgtcg gtgcctaact
     5701 tatcaatcat caggaaggtg tctttgccgt atttttccgc aatatcgtag atatcccggt
     5761 gcatatattc cccggcaacc ggcagatttt cgaagttagc cagaatatga cggcggattt
     5821 cggtcagcac ttccggctgg ttggtgccga tataaaacac ctgctgattt ttttccgctt
     5881 cgaaggtatc tagacgtacc gcaaagacgg ccagcttccc ggcgcaacca gaagattcaa
     5941 ataaccgatc cggatcggcg ttataacgtg cgggcgtgtc ggcttcaata tcacgaacgc
     6001 ggtggacata gtcataatcg tgggcgtgac gaccatcgtg acgcacatcg tcatctttga
     6061 tgcgatcatc atccagcttg ctaaggatct gctccggcgt ttcgcccaga tcaatcccca
     6121 gatggttcac cagcgtcagt ttgccgtctt catttatacg cgcgaataac gacatttcgg
     6181 tatacgccgg gccacgttgc accagcgagc cgccggagtt gttacaaata ccgccgataa
     6241 ccgatgcgcc tatacacgac gatccaatca ccgagtgcgg ttcgcgtccc agcggtttga
     6301 gggctttttc cagcgaatag agcgtggtgc ccggataggc cagcacctgt tcgcccttgc
     6361 caagaacgtg cagcttgtcg agacgcaggg tgctgatgat cacgatatcg cgatcatagt
     6421 cgttaccgtt tggcgtcgat ccttcggtca ggcctgtatt ggcagcctgc atcagaataa
     6481 ttttgtcggc ggtgacacag gctttcagca cccgccacaa ttccagtagt gagccaggga
     6541 aaacgacagc cagcgcgtcg ccctgaccag aacggaagcc cttgcgatag cgggccgttt
     6601 ttgcgggatc ggtgagcagg tgtgaatgac cgaccagacg ggcaagttca ttcaaaaagg
     6661 ctttattatc agttgttgtc atggaagaca ttttccactc cttgtggtgg cgaaaaaata
     6721 tcacaaacta gaatagcgcc ttgtatgaca attcagcgag tgaatatttt gaaaattcag
     6781 acaataaact gagcattctt tttctctctc catcatgctt atggcaaact acggtttttg
     6841 caccattttc gctgcctcgt cgggcaacag aggaagaaaa atccatatga aatggctatg
     6901 ttcagtagga atcgcggtga gtctggccct gcagccagca ctggcggatg atttattcgg
     6961 caaccatcca ttaacgcccg aagcgcggga tgcgttcgtc accgaactgc ttaagaaaat
     7021 gacagttgat gagaaaattg gtcagctgcg cttaatcagc gtcggcccgg ataacccgaa
     7081 agaggcgatc cgcgagatga tcaaagacgg tcaggttggg gcgattttca acaccgtaac
     7141 ccgtcaggat atccgtgcca tgcaggatca ggtgatggaa ttaagccgcc tgaaaattcc
     7201 tcttttcttt gcttacgacg tgctgcacgg tcagcgcacg gtgttcccga ttagcctcgg
     7261 tctggcctcg tcttttaacc tcgatgcagt gaaaacggtc ggacgtgtct ctgcttatga
     7321 agcggcagat gatggcctga atatgacctg ggcaccgatg gtcgatgtct cgcgcgatcc
     7381 gcgctgggga cgtgcttccg aaggttttgg cgaagatacg tatctcacct caacaatggg
     7441 taaaaccatg gtggaagcga tgcagggtaa aagcccggca gatcgctact cggtgatgac
     7501 cagcgtcaaa cactttgccg catacggcgc ggtagaaggc ggtaaagagt acaacaccgt
     7561 cgatatgagt ccgcagcgcc tgtttaatga ttatatgccg ccgtacaaag cggggctgga
     7621 cgcaggcagc ggcgcggtga tggtggcgct gaactcgctg aacggcacgc cagccacctc
     7681 cgattcctgg ctgctgaaag atgttctgcg cgaccagtgg ggctttaaag gcatcaccgt
     7741 ttccgatcac ggtgcaatca aagagctgat taaacatggc acggcggcag acccggaaga
     7801 tgcggtgcgc gtggcgctga aatccggaat caacatgagt atgagcgacg agtattactc
     7861 gaagtatctg cctgggttga tcaaatccgg caaagtgacg atggaagagc tggacgatgc
     7921 tgcccgccat gtactgaacg ttaaatatga tatgggattg tttaacgacc catacagcca
     7981 tttggggccg aaagagtctg acccggtgga taccaatgcc gaaagccgcc tgcaccgtaa
     8041 agaagcgcgt gaagtggcgc gcgaaagcct ggtgttgctg aaaaaccgtc tcgaaacgtt
     8101 accgctgaaa aaatcggcca ccattgcggt ggttgggcca ctggcggaca gtaaacgtga
     8161 cgtgatgggc agctggtccg cagccggtgt tgccgatcaa tccgtgaccg tactgaccgg
     8221 gatcaaaaat gccgtcggtg aaaacggtaa agtgctgtat gccaaagggg cgaacgttac
     8281 cagtgacaaa ggcattatcg atttcctgaa tcagtatgaa gaagcggtca aagtcgatcc
     8341 gcgttcgccg caagagatga ttgatgaagc ggtgcagacg gcgaaacaat ctgatgtggt
     8401 ggtggctgta gtcggtgaag cacaggggat ggcgcacgaa gcctccagcc ggaccgatat
     8461 cactattccg caaagccaac gtgacttgat tgcggcgctg aaagccaccg gtaaaccgct
     8521 ggtgctggtg ctgatgaacg ggcgtccgct ggcgctggtg aaagaagatc agcaggctga
     8581 tgcgattctg gaaacctggt ttgcggggac tgaaggcggt aatgcaattg ccgatgtatt
     8641 gtttggcgat tacaacccgt ccggcaagct gccaatgtcc ttcccgcgtt ctgtcgggca
     8701 gatcccggtg tactacagcc atctgaatac cggtcgcccg tataatgccg acaagccgaa
     8761 caaatacact tcgcgttatt ttgatgaagc taacggggcg ttgtatccgt tcggctatgg
     8821 gctgagctac accactttca ccgtctctga tgtgaaactt tctgcgccga ccatgaagcg
     8881 tgacggcaaa gtgactgcca gcgtgcaggt gacgaacacc ggtaagcgcg agggggccac
     8941 ggtagtgcag atgtacttgc aggatgtgac ggcttccatg agtcgccctg tgaaacagct
     9001 gaaaggcttt gagaaaatca ccctgaaacc gggcgaaact cagactgtca gcttcccgat
     9061 cgatattgag gcgctgaagt tctggaatca acagatgaaa tatgacgccg agcctggcaa
     9121 gttcaatgta tttatcggca ctgattccgc acgcgttaag aaaggcgagt ttgagttgct
     9181 gtaatgcttt agtaagggcg cgacgtttgc gccctttgta ggccggataa ggcgctcgcg
     9241 ccgcatccgg caggaaacgc cggaccgaat atgcctctta agtatttatc tgattctgca
     9301 actgaatcct tccgctcaag ctaaccccgc cattatcaac tatgcttttc tcttaattcg
     9361 ctgaaaacaa cagtaaaaga atgaggaaag catcatgcca ctcttaaagc tctgggcagg
     9421 ttcactggtt atgttggcag ccgtgagcct gccgctacag gcggcttccc ccgttaaagt
     9481 cggttcaaaa atcgataccg aaggtgcgct actcggcaat atcattttgc aggtgctgga
     9541 aagccacggc gtaccgacgg tgaataaagt gcaactcgga acgactcctg tggtgcgcgg
     9601 ggcgattact tccggtgaac tggatatcta tccggaatat accggcaatg gcgctttctt
     9661 ctttaaagat gaaaacgatg cagcgtggaa aaacgcgcag caaggttacg agaaagtcaa
     9721 aaagctcgat tcggagcaca acaagttaat ctggctgacg cccgcgcctg caaataacac
     9781 ctggactatc gccgtgcgtc aggatgtggc agagaaaaat aaactcactt cgcttgccga
     9841 cctgagtcgt tatctgcaag agggcggcac cttcaaactg gcagcctcgg cagagtttat
     9901 cgaacgcgcc gatgcgttac ccgcgtttga aaaagcctat ggctttaagc tcggtcagga
     9961 tcagttgctg tcactggctg gtggcgacac ggcggtgacg atcaaagcca ctgcccagca
    10021 aacctctggc gttaatgctg caatggctta cggcactgac ggcccggtag cggcgctggg
    10081 gctgcaaacc ttaagcgatc cgcaaggcgt gcaacctatc tatgcgcctg caccagtggt
    10141 gcgtgaatcg gtgttgaagg agtatccgca aatggcacag tggctacagc cagtcttcgc
    10201 cagcctcgat gcaaaaacat tgcagcaact gaatgccagc attgcagttg aaggactgga
    10261 tgccaaaaaa gtggctgccg actacctgaa acaaaaaggg tggacgaagt aatttcccgt
    10321 gacttatcta cgtattaatc ctgttctggc gctgctgctg ttgctgacgg taatcgcagc
    10381 ggcgctgccg tttatcagtt acgcgcctaa tcgtttagtt tcgggtgagg ggcgtcatct
    10441 ctggcagctg tggccgcaaa cgatctggat gctggtgggc gttggttgcg cctggctgac
    10501 ggcctgtttt attcccggta aaaaaggcag catttgtgca ctcattctgg cgcaattcgt
    10561 cttcgtattg ctggtgtggg gcgcgggaaa ggcggcgaca caactggcgc aaaatggcag
    10621 tgcgctggcg cgtaccagcc tcggcagtgg tttctggctg gctgcggcgc tggcattgct
    10681 ggcctgtagc gatgccatcc gccgaatctc cacgcatccg ctgtggcgct ggttgttgca
    10741 tatgcagatt gccattattc cgctgtggtt gctgtactcc ggcacgctta acgatctctc
    10801 actaatgaaa gaatacgcca accgtcagga tgtgtttgac gacgcgctgg cacaacatct
    10861 gacgttgctg tttggtgcgg tgctgcctgc gttagtgatt ggtgtgccgt tgggcatctg
    10921 gtgctacttt tccactgcgc ggcagggggc aattttttct ctgctcaatg tcattcagac
    10981 cgtgccttcg gtggcgctct ttggcctgtt gattgcgccg cttgccgcgc tggttacggc
    11041 ctttccgtgg ctggggaagc tcggcatagc aggaaccgga atgacacccg cactgattgc
    11101 gctggtgctc tatgccttgc tgccgctggt gcgcggcgtg gtagtcggtt tgaaccagat
    11161 cccgcgcgat gtgctggaga gcgccagagc gatggggatg agcggggcgc agcgtttcct
    11221 gcatgttcag ttaccgctgg cgttaccggt atttctgcgc agcctgcggg tggtgatggt
    11281 gcaaactgta ggtatggcgg tgattgcggc gttaatcggc gcaggcggtt ttggtgcgct
    11341 ggttttccag gggctgctaa gcagcgccat tgatttagtg ttgctggggg tgatcccggt
    11401 aattgttctg gcggtgctga tcgatgccct gttcgatttg cttatcgcac tgctgaaggt
    11461 gaaacgtaat gattgaattt agccatgtca gcaaactgtt tggcgcacaa aaagccgtta
    11521 acgatcttaa tctcaatttt caggaaggga gtttttcggt gctgattggc acatctggct
    11581 ccggcaaatc caccaccttg aaaatgatta accgcctggt ggaacatgac agcggagaga
    11641 tccgctttgc cggagaagaa attcgctcgc tgccagtact ggagttgcgc cgccggatgg
    11701 gctatgccat tcaatctatt ggcctgttcc accactggag cgtggcgcaa aacatcgcta
    11761 ccgtgccgca attacaaaaa tggtcgcggg cacggattga cgatcgtatc gacgaattaa
    11821 tgacgctact ggggctggag tcaaatttgc gtgagcgtta tccgcatcag ctttccggtg
    11881 gtcagcagca acgtgtggga gtggcgcgcg cactggctgc cgatccgcaa gtcttactga
    11941 tggatgaacc ttttggcgca ctggacccgg taacgcgcgg cgcgttgcaa caagagatga
    12001 cgcgcattca ccgtttgctg gggcgcacta ttgtgctggt cactcatgat attgatgagg
    12061 cgctaaggct ggcagaacat ctggtattga tggatcacgg tgaagtggtg cagcagggga
    12121 atccgctgac gatgctgact cgtccggcga atgattttgt ccgccagttt tttggacgta
    12181 gtgaactggg tgtgcgcctg ctttcgttac gtagtgtggc ggattacgtg cgtcgcgaag
    12241 aacgggcaga aggtgaggca ctggcagaag agatgacgct acgcgatgcg ctctccctgt
    12301 ttgtcgcgcg gggatgcgag gtgctgccgg tggtgaacac gcagggccag ccttgcggca
    12361 cgctgcattt tcaggatctg ctggaggagg cgtaagcgta tgaagatgtt gcgcgatccg
    12421 ctgttctggc tcattgcttt gtttgtggca ctgattttct ggctgcctta cagccagccg
    12481 ctgtttgctg ccttgttccc acaactgcca cgacccgttt atcagcaaga aagttttgca
    12541 gctctggcac tggctcattt ctggctggtg ggaatttcga gtttgtttgc ggtgatcatt
    12601 ggcactggtg ccggaattgc tgtcactcgc ccgtggggcg cggaatttcg cccactggtg
    12661 gaaactattg ccgccgttgg acagactttt ccgcctgtcg cagtgctggc gattgccgtt
    12721 ccggtgatcg gctttggtct gcaaccagcg attatcgcct tgatccttta cggtgtgctg
    12781 cccgtcctgc aggcgacact tgccgggctg ggagcgattg atgccagcgt gacagaagtt
    12841 gcgaaaggta tgggaatgag tcgtggtcag cgactgcgta aggtcgagct accgctggcg
    12901 gctccggtga ttctggcggg cgtgcgaact tcggtgatta tcaacattgg tacggcgacg
    12961 atcgcctcaa cggtaggggc cagcacgctg ggtacgccca tcatcatcgg gcttagcgga
    13021 tttaataccg cgtatgtgat ccagggggcg ttactggtgg cactggcggc gatcatcgca
    13081 gaccgcctgt ttgaaaggct ggtgcaggcg cttagccagc acgcaaaata aaggtataac
    13141 ctgcgagcat gacgccacca attccgccta acgccataaa caggaacagg gcgatgaccc
    13201 caattttagc tatgcgcata ttgcactcct tatgttaacg aaaggattgt acagtaaagc
    13261 gcatttgtta acgaatcatt aaatgccgag tgggaaaata tcatggcctt gttcttgcca
    13321 actggtgagt tgctgctgtt gggcggaggt tcgattttca ccgcaccaca ccagcaatgt
    13381 acggccttcg aatagttcag ggcgtagttg attgagcgag tgggcgagga catcaatgcg
    13441 ccatccttgt tgactggcaa tccagccctc cagccacaga cgggtggtat cctgaatatt
    13501 ccagccaacc accagcgcat ctttaccctg ttttttacgt gccgaagcca gacaaatggc
    13561 gatgtagttg atcagtacgc cgtcgaggat cgccagcagc gcctggagag tcggttgttg
    13621 gcactgaagc cgtcggcgca gaggaataaa cagatgtgtg gtgagtgtct gggcggggta
    13681 atcctgaccg cgctctttga tccacgttcg caggctatgc agattgccgc tttgcaggta
    13741 ggtcagtaat gtttcttgct gatcgcgcca gccgttctgc acatcaacat tttcattact
    13801 gagcagcatt ttaactttgc tgacctgcac gccgttgtcg atccagcgtt tgatctcgcg
    13861 gatccggtca atatcggcat cgttgaacag tcgatgaccg ccgtctgtcc gttgcggttt
    13921 cagcaacccg taacgcctct gccacgcgcg taacgtgaca gggttaatat cacaaagcaa
    13981 cgccacttca ccaattgtgt aaagcgccat cgtctcaccc ttgctcgcga ggtcccggtt
    14041 taactttaga cgcagttttg cgaaccaggt agttttgccc gttttttgtg catctatagg
    14101 gtgattttat ttttgccagg cgattttgag tgatcgtact cacgaattct catttttctg
    14161 caagagttca aagaaagtta aacgcaggca atgtatgtta cgcgttttaa agggaagtgt
    14221 ggtttgcggg tatgtacgat tttaatctgg tgttgctgct gcttcagcag atgtgcgttt
    14281 ttttagtcat tgcgtggtta atgagtaaaa cgccattatt cataccgtta atgcaggtca
    14341 cggttcgtct gccgcataaa tttctctgct acatcgtctt ttccatcttc tgcatcatgg
    14401 gcacctggtt tgggttgcac attgacgatt ctattgccaa tacccgtgcg ataggcgcgg
    14461 taatgggcgg cttactcggc ggtccggtcg tcggtgggct ggttggtctg accggcggct
    14521 tacatcgata ttcgatgggg ggcatgaccg cgctaagttg catgatctca accatcgttg
    14581 aaggattgct cggcggcctg gtacacagca tcctgatccg tcgcgggcgc actgataaag
    14641 tctttaaccc cattaccgcc ggtgccgtca cgttcgtcgc tgaaatggtg caaatgctga
    14701 tcatccttgc gatcgcccga ccttatgaag atgcggtgcg tctggtgagt aatattgctg
    14761 caccaatgat ggtcaccaat accgtcggcg cggcgctgtt tatgcgtata ttgctcgata
    14821 aacgcgcgat gtttgaaaaa tacacttctg ctttttctgc cactgcgctg aaagtggcag
    14881 cctcgacgga aggcattttg cgacaggggt ttaacgaagt gaacagcatg aaagtggctc
    14941 aggtgctgta tcaggagctg gatattggtg cagtcgcgat taccgatcga gagaaattgc
    15001 tggcctttac cggaattggt gacgaccacc atttacccgg caaaccgatt tcttcgactt
    15061 acaccttaaa agcgattgaa accggtgaag tggtctacgc tgatggcaac gaagtacctt
    15121 atcgttgctc tttgcatccg caatgcaaac tggggtcgac gctggtaatt ccgttgcgtg
    15181 gcgaaaatca gcgcgtgatg ggcaccatca aattgtatga agccaaaaac cgtttattca
    15241 gttcaataaa ccgcacgctg ggcgagggga ttgcgcaact gctttcggcg cagattcttg
    15301 ccgggcaata tgagcggcaa aaagcgatgc tcacccagtc agaaatcaaa ctgcttcacg
    15361 cccaggtgaa tcctcatttt ttgtttaatg cgcttaacac cattaaagcg gtgatccgcc
    15421 gcgacagcga acaggccagc cagctggtgc agtatctttc cacttttttc cgcaaaaact
    15481 taaagcggcc ttcggagttt gttactctcg ccgacgaaat tgaacatgtg aacgcttatc
    15541 tgcaaattga aaaggcgcgc ttccagtcgc ggttgcaggt caacattgct attccgcaag
    15601 aattatccca gcagcaattg cccgcgttta ccctgcaacc gatagtggaa aacgccatta
    15661 aacatgggac atcacaactg ctggatacag ggcgagtggc aatcagcgcc cgacgtgagg
    15721 ggcaacattt gatgctggag atcgaagaca atgccggttt gtatcaaccg gtaaccaatg
    15781 ccagtgggct ggggatgaat ctggtggata agcgtttacg tgaacggttt ggcgatgact
    15841 atggaataag cgtcgcctgt gagcctgata gttacacccg aataacgtta cgactaccat
    15901 ggagggacga ggcatgatta aagtcttaat tgtcgatgat gaaccgttag cacgggagaa
    15961 cctgcgcgta tttttgcagg agcagagcga tattgaaatc gttggagagt gttcaaacgc
    16021 cgtggaaggg atcggcgcgg tgcataaact gcgcccggat gtgctgtttc tcgatatcca
    16081 gatgccgcgc atcagtggtc tggaaatggt ggggatgctc gacccggaac atcgtccgta
    16141 tattgttttt ctcaccgcgt ttgacgaata cgctattaaa gcctttgaag aacatgcctt
    16201 tgattatctg ctgaagccaa ttgatgaagc gcgactggag aaaacgctgg cgcgtttgcg
    16261 tcaggaacgc agcaagcagg atgtttcgct gttaccggaa aatcaacagg cgctgaaatt
    16321 tatcccttgt acggggcata gtcggattta tttgctgcaa atgaaagatg tggcatttgt
    16381 cagcagtcgg atgagcggtg tctacgttac cagccacgaa gggaaagagg gctttaccga
    16441 attgacatta cgtaccctgg aaagtcgtac accactactg cgctgccatc gtcagtatct
    16501 ggttaacctc gcgcatttac aggagattcg tctggaagat aacggccagg ccgagttgat
    16561 tttgcgtaat ggcttaaccg tgccggtcag ccgccgttat ctgaaaagct taaaagaggc
    16621 gattggcctg taaaagactg ctaaaatggc tttttgcctc atcaacacct gaaggcctca
    16681 tgctaagtaa cgatattctg cgcagcgtgc gctacatttt gaaagccaat aataatgacc
    16741 tggtgcgtat tctggcgctg ggtaatgtcg aagccaccgc ggaacagatc gccgtctggc
    16801 tacgtaaaga agacgaagag ggttttcagc gttgtccgga cattgttttg tcgtcattcc
    16861 tcaatggcct gatttatgaa aaacgcggca aggatgagtc tgctccggca ctggagccgg
    16921 aacgtcgcat taataacaac atcgtgctga aaaaattacg catcgcgttt tcgctgaaaa
    16981 ccgatgacat tctggctatc ctcaccgaac agcagttccg cgtttcgatg ccggaaatta
    17041 cagcgatgat gcgtgcaccg gatcataaaa acttccgcga atgcggcgat caatttttac
    17101 gttattttct gcgtggactg gcagcgcgcc agcatgtgaa aaaaggctaa aaaattggcg
    17161 gtcatgttta gagcatgacc gccaaccgat tatttcactt ctttaaaacc agcggctttc
    17221 atcaccagtt ccatttgcgc catagtgata ccttttttgg catcttcagc agaaacgttg
    17281 attcctgaaa taccctgcag ggctttaaaa tccacttttt ccatatcgat agtcacgttt
    17341 tcctgcgcgt aggtatctgt ataggttaat ttttcttcaa cacccgcgat gtttttgtat
    17401 ttggcgctta acggctcaag tgtcttggca gcgtcttctt tggtggttgc accaatagaa
    17461 gcaaattgaa ttttggtttc agaagattgc ttaagcacct tgtcaccttt gtagacatag
    17521 gtaatggcaa tttcagtgcc gttcagattg gcgctgaatt tcttcgattc ttctttgtca
    17581 ccgcagccag caagagagaa aaccagaaca gatgcaacaa cgagggaaaa cagcttattg
    17641 aaagccttca tgtaaaactc cattttattt aatcaagaaa ctggtgactc tcaccagggg
    17701 ctatatagaa tatgcctaat accgtgacgt gagcagtccg gaactggagt agaactctta
    17761 gtaaaaagca ctatttcatc cttgttgctg aagcatgggg aataattgtt cgcaaagtaa
    17821 aacaccgtta ttcattgctt ctacccgtgc ctcgctttct gtattacgaa attgtcccaa
    17881 cacatgtgcc agccgataaa aaccgaccgc ggagaggtca ttcgccagca actctgcctg
    17941 actaatagcg ctctgttcct gatagcgcca gccgttatgg agcagttgaa taagtaacgc
    18001 ctggcagcgc atcagcaact gatgagcggt agacggcaca ggcaaaacgc tggcagaagg
    18061 tagcggtgcc acaggcgcag tttccgcgtc cagcgcccag gcgcgggttt ttgtcatcat
    18121 cacccgtggt tccagtgtca attgcccatc aacaaaactg acaaagccag aaaccagact
    18181 cacggggtcg tctgtttgtt gcaaaagcgc cgccatgcgt tcaacggcaa aaggagaaca
    18241 ggcagatgcc ggaagggata acgtcaggag attatcttca ccttcgccgc tgattacctg
    18301 cgcatccagc gtctggcggc tgctatccca accgagcgaa atacactcag cgaccggcag
    18361 aataaataag ttatcgacct gattaagagg ccgtatgcag gcggggggac gctggcgtaa
    18421 atattcccgc aaagccacaa tgcccggctg gcgtaacggc gcgctcaaca tttgccaggc
    18481 atcaggcgac agcggcacaa cgctgcttaa gcggttgcgg gtagctaaca gcagctcgcc
    18541 atcggcactg cgttttgctg cttgtgaaac aatttgccca cccgccagtg cgccagcctg
    18601 aaaactaaac agccgacgcg tagctgccgg tgagttttcc tgttcacttc gcggccaact
    18661 gcgcgaaagg tgcaaaatac tgccggtgtc gggatcggta aaccagatgc gtaaaccata
    18721 atgctcaata tcctgccagc aacgcatacc taaagacacc agccgcagat gatcaagctt
    18781 tgcttctccg gcaatgccag agccaacgac cgtgcgccac ggcacaggag gaacttcacc
    18841 aacactgtcg cgccgggcca tctcttgtgc gcaatttaat cgactgttta atgccgcaag
    18901 ctgacgtaag cattctccgg catgatagtg gctggcgcga gtgtggaagg catcaacgct
    18961 tgcccgtagc tgtcgtagtg attcactcac ccagcgccag ttgcaggcct ctgccgcctg
    19021 caatgcgcgg ttgaatgctg cctcgtagtg aataagcggc tggctgatgc cgccaagcca
    19081 taatgcctgg cttaattgct gaacatattg acgacacgtt tggccttctt cgctggcaaa
    19141 cggatcgtca gatgatgtga cgtgttcgct gcgcatctgc cagattaaat ggttaaattc
    19201 tgcttgctgc gctttggcct cgacgaaggc ctgcacagcc agtacgacat gttcgcaaag
    19261 tgtgccttca atacaatcac aacgggcgaa acgaatactg ctacgggaat aaaaacgcac
    19321 atcgctcatc ggtaagcggg cagagggaat ttcgcccggc gcacagaaca actcaatggt
    19381 gatgccttta gcgaccagcg cctgcgcgcg tttgcgggtg gcatcgggca gggtagccag
    19441 ttcttccagc cagattgccg gatcccacgc ttcttctttt tccgtaggct gggcggtagt
    19501 acaaagtcgt tgataactta acacaagcat cacgcgatga cggcacatac cgctggcccc
    19561 gcagctgcac tgagcctctt tcagtgcctg gccgttcgcc agctgggtac ggacaccgtc
    19621 actgaaggtg gcgattaaag cgctgttctc atggctgatt tccgggacgt tgccattttc
    19681 cagttcctta agactgcgct taacaaaacc ggcattgctt aacgccgtca gggcctgcgg
    19741 tgtcagttct aataattccg gacgtagtga attcatgact gaagattctc cgcaagccat
    19801 gatgccagct cgcccggtgt catggcggct atttgtgcgc cgacatttac cagcgcctgg
    19861 gccgtatcgt ggtcatagca aggtgttgct gtgctatcga gcgctgccag tcccagcact
    19921 ttgatgccgc tctggacaca ctttttcacc tgatgcgtca gtaatgatga tgaaccccct
    19981 tcgtaaaaat cgctcacgag gataatgacg cttttcgctg gttgttcaat aagttgccga
    20041 ccatactcca cggcactggc gatattggtc ccgccgccca actgtacttt cattaataac
    20101 tctaccggat cggcaacgtc tgccgtgaga tcaacgacgc ttgtgtcaaa cgccaccaga
    20161 tgggtacgaa tgccgggtaa ctgccacaaa caggccgcca tcaccgcaga gtggatcacc
    20221 gaatcgacca tcgatccgct ttgatcaacc agtaagacca gttgccattg ttcgctttgg
    20281 cgtttaatgc ggctgttaaa gcggggggat tcgatataca acttgccgtg ttgcgggtgc
    20341 cagtgttgca ggttggcgcg cagagtactt ttgaaaacaa agtttcgcgc cagtggaata
    20401 aatgaacggc gacggcgatc gcggacacca gaaaaagcct ggcgaacttc ctttgccagt
    20461 cgcgccataa tttcttcaac aacctggcgc actatctggc gggcggcagc cagtacttcg
    20521 ggattcatca gatgtttggt gtgcaaaacg gcgcgtagca ggctttcgga aggctgcata
    20581 cgttccagca cgtcgagatt tgtcaccaca tcttcaatgc cgtagcgcag tacggcatcg
    20641 ctttccagcc gctcaatcac ctgttgcgga aacagcgtgt ggatactgtt gatccactca
    20701 ggagtggtga gatttgagcc acctaatcca ccagagcgtt caccacgctg gagtcgttca
    20761 ggatcgcgcc catacagcca ctccagcgcg tgatctatct gccgggcgtt gtcatccagc
    20821 ccacaaagcg tcgtttctgc cgcttcgcca agaattaatc gccagcgttg tagctcacgg
    20881 gtggtcagaa gatcgttcag tttagacatg gaaaactccc cagtgttgca gcgatgccag
    20941 tgcctgctgt tcgagttgtt gatgatgtgc aatggcttgc ggtggacaat gcaacggcat
    21001 ttgcagtgcc gaaacgggaa gttgcgccag ttgataatgc tcaagcacct gatgcgccag
    21061 cgttccgcgt tctcgtggcg gtagccaggc catcgccgcg cgtaaatcgg gcagggcatt
    21121 gataaaatcg gcatcactta gttgatttaa atgactgctg aaaccggcga taaatgtcgg
    21181 ctgacaggcc agttgatgac gggctaatgc cagcaaacct tgcagcgcct cgccggattg
    21241 tgccggggat aactgcgcca gcatcgtcag cgccgcttcg gcactggtgt tgggatgttc
    21301 cagacgcatt agtgcgccaa gaaccgcgcc gcgatccagc gccggggcat gaatcgcctg
    21361 actgcgtcgc tccagtaagg ctaccgccgc agaaagcgaa acgccggata aattaacgcc
    21421 gctatgtaga tcgcgcagaa tatggcaaag cgcttgccag ctatgcaggt gagcgtgaaa
    21481 ctccttttca tccggtctgc cgttagattc acacagccac agcgtgcgat cgatagccgc
    21541 gcataacgtc gtttgtaata tctgcgcgcc ttgcataccg ctaatttcat ccagccgcca
    21601 taaggcatat agcacttcca gcgcctggcc catttcggca aattgatttt cctgggcgat
    21661 taataatgtg agttgctcca gtaattgttg actgaaggac gccagacccg ctaacgccgc
    21721 ttggctaaga catgtggtga tactgccgat tccacccgcg tccagcatat cggcttctaa
    21781 tttatggcgt gcggcttcct ggagtgtggc accaaagcag gcggcctcaa tcaatgccgc
    21841 atgctggcta agcgggcggg ttaatttcca gtgttcttca ccgttgcctg caagtgtcag
    21901 tgtgcttccc tgctggcgca caatccctgg gatctccagt attgccagcc gatgtaacac
    21961 ctgactttgc gctagtccat tggggttaaa gcgatttaag gtaagctccg ccggaaggga
    22021 gattgcggta cgttccagtt cgcaggtgac atcttttggc agaggaggtt gtggcgtaga
    22081 aggggcaagt ttaccgaatc cgtcacccgc taatgtgtct atcaacgtta gcagaatcgg
    22141 atcggtgtcg ggatgaataa cgccgcgata gctccacggc aacggcgcat tcagggcttc
    22201 tttaatcagc gatcctgcta tggcatccag ccagtcagtg cgtaacggta gtgtatgacc
    22261 gcgcaactgt gccagtgcca tcgcatgcag atgagcggca gccatatccg cggtcgaagc
    22321 aggcaacttg tgctggcgca aacgggtgag aaccgttttt agcagttgtt ctcccgcctg
    22381 ctgtaagccc cactgccagc accagttttg ccagaccggg gcaggcattc cggaaaggta
    22441 tcctgccagc acatcaaggc gcttttcact gtagggggtg agataacaac ctgtaacggc
    22501 atctgccagc gacggcaatt ctggcgtgtt aatttcctgc gggcattcgc gccacatttt
    22561 tgccagtacc ggagcgtgcc agccaccgca gacaactaac acgtcgccat tattttgctg
    22621 catcgcccat ccaatccagc gggccataaa ggcttcgcgc agacgattga gagcatctcc
    22681 gggggagtcg ccccgcagtt gggcaaaata gtgcgccagt gcagagggta atgcagtttg
    22741 ctggctttca tcttcgaaca agtgatccca cagggtgtcg ctgttatcca tgcgggtggc
    22801 acgcagcagc aaggcctggc tttcctcttg cgtatcaggc gaatcgtcca cttcttcact
    22861 ctgcgcccag caaggcaaat cgatgaagta agtttgtgcc tgaatacgac gtgcggcttg
    22921 tagcgcctgc cactccggcg aaaattcagc aaatggcgtc caggcaccac gcccgggggc
    22981 tgcaccgtcc tgatactggc aataactgta aatagctacc ggaagctggt gggctaaaaa
    23041 cagttcgtct acccggtcat taaaatcagc cgggccttca atcaatacgt atcgtggccg
    23101 ctggctttcg attaacgatt tcaccagccg ggcgcaggcc ggactatgat gccggatgcc
    23161 gacaattaac ggctcgctca tgcatctttc ctcacggcag gcggtggcgg gcttgataat
    23221 aagcctgcca gtgagcttct ttatgtgtgg cgactcgttg ttcgaagtga cggcgcagac
    23281 gagcgcgatc ttcttcgtta tctttgacga tggttccggc aatgcagtcc accaggtcgg
    23341 ctggctcacc tgcgcgattc gctaaaaacc aggcgcggac gcctacggcg tgggccacgt
    23401 tcacggcttc agcggtggac ataatcgctg ttagtgtatc cattgacgtt ttcttttcgc
    23461 cgttggcacg cagatcgcgg aaggtgcgga ccagtaactc cagtaccgcg tcaggcactt
    23521 tatgcggaat accgctatgg gccagcaaac gcgccgaagc actggcgaca agctccagct
    23581 cctgggcgaa atccataatc ggaaagacgg tttcaaaatc gaagcgacgc tttagcgcgg
    23641 cactcatctc attgacgccg cgatcgcggg tattggcagt ggcaataata ttaaagcctt
    23701 cccgcgcata gagctgactg gcttcaccgg tgagttccgg caccgtcatc accctgtcag
    23761 aaagcatacc taacagacag tcttgtactt ccagcggtgt acgcgttatc tcttcgaaac
    23821 ggacgatttt gccgtcgcgc attccctggt aaagcggggc agggaccagc gcttccgttg
    23881 atgggccatg attgataagc agagcgtagt tccagccgta cttgatttga tcttcagtgg
    23941 tagatgcgcc gccctggatg gttaacccgg catcgccgct aatagcggtt gccagtaatt
    24001 cagacaggag agattttgcg gtgccaggtt cccccaccaa catcaaaccg cgtccggtgg
    24061 caagagtgac taacatacgt tcgacagcgg agggactaat caccacttta cggctaatat
    24121 tttgcgcgct atcgccaagg ataaaagcac gggccgcagg caaacttaac tgccaaccgg
    24181 gcgggcaagg agcgttatca ttttgtttta attttgccag ttcatcggcg tataacacag
    24241 cagcaggcgg acgctgcaga tgattattct gcggtgacat atattcatcc ctgaaatgta
    24301 ttaatgaaat aaacgatatt ttacgcgcaa taaatgtttg gcgcattagt tgtttgagca
    24361 aaaaatatct ggaaagcaga tcgcagtaaa taaaaaaacc tgctggtgag gcaggttctt
    24421 ttatttacta ttttcaggcc taacaccaca gcctgtgcaa catcgtgttc caacgaacga
    24481 attataacca cggctggacc ccgttgcaat taagataatg agatttataa tccagaccgt
    24541 tgggacgttg cgccggatcg aacctatttc aagtcctggc taccggacgg ggggtgctgc
    24601 aggtggtgcc ctgatccgga gtgcaacatc gtggtccaac atacgtaaac cagtatcgac
    24661 aggcagtgta agggtaaggt gctggtgttg cgctccttaa taaggagagc ggatggactc
    24721 gctgacacaa aagttaaccg tgctcattgc tgtactggag ctattagtgg
//
LOCUS       DAPGWD010000060        23836 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPGWD010000060 DAPGWD010000000
VERSION     DAPGWD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.060"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            327..2999
                     /gene="rcsD"
                     /locus_tag="RHX88_002335"
     CDS             327..2999
                     /gene="rcsD"
                     /locus_tag="RHX88_002335"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDV2231023.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            3016..3666
                     /gene="rcsB"
                     /locus_tag="RHX88_002336"
     CDS             3016..3666
                     /gene="rcsB"
                     /locus_tag="RHX88_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDV2231024.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3866..6715)
                     /gene="rcsC"
                     /locus_tag="RHX88_002337"
     CDS             complement(3866..6715)
                     /gene="rcsC"
                     /locus_tag="RHX88_002337"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDV2231025.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(6990..7766)
                     /locus_tag="RHX88_002338"
     CDS             complement(6990..7766)
                     /locus_tag="RHX88_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDV2231026.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(7771..9420)
                     /locus_tag="RHX88_002339"
     CDS             complement(7771..9420)
                     /locus_tag="RHX88_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDV2231027.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLPTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(9421..13935)
                     /locus_tag="RHX88_002340"
     CDS             complement(9421..13935)
                     /locus_tag="RHX88_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDV2231028.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPFEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTNKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQAIGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(13959..14582)
                     /locus_tag="RHX88_002341"
     CDS             complement(13959..14582)
                     /locus_tag="RHX88_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDV2231029.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGIASQYLPPEMTLKPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWILNDSNPNFIGIYRLNFLAR"
     gene            complement(14579..16267)
                     /locus_tag="RHX88_002342"
                     /pseudo
     CDS             complement(14579..16267)
                     /locus_tag="RHX88_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(16416..19043)
                     /gene="gyrA"
                     /locus_tag="RHX88_002343"
     CDS             complement(16416..19043)
                     /gene="gyrA"
                     /locus_tag="RHX88_002343"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDV2231030.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            19190..19912
                     /locus_tag="RHX88_002344"
     CDS             19190..19912
                     /locus_tag="RHX88_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDV2231031.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(20040..23774)
                     /gene="yfaL"
                     /locus_tag="RHX88_002345"
     CDS             complement(20040..23774)
                     /gene="yfaL"
                     /locus_tag="RHX88_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDV2231032.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSVSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSMTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVS
                     QSQGDDIQMRLGLHSEWRTAVHVIPTLDLNYYHNPHSTEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
BASE COUNT         5713 a         6504 c         5819 g         5800 t
ORIGIN      
        1 catccggttt tttttaccct tctttacaca cttttcatta ttctgtgcta ccacagaaaa
       61 actataacgc ttgttaacta tttcacaaat aattaacatc agcataattt ccagcaatct
      121 ttgtttattt gcaattattt ttgttgggct ttttgtaggt tatttgtaca gcaaaatggc
      181 gcttgtacat ctatttcccc caatgcagga tgataaatat cacgggagaa tagagaatca
      241 tcaatcaggt aagagtctgg aatttcacac tgtacccttt atactgccct atcacttcgc
      301 gaagttttaa caggtcataa acacgaatgc gtcagaaaga gacaacggcc acgacccgct
      361 tttcactcct accggggagc attacccgct tctttttact gttgatcatt gtgttactgg
      421 tgacgatggg ggtaatggta caaagcgccg ttaacgcctg gctgaaagat aaaagttacc
      481 agattgtcga cattacccac gctatccaaa agcgcgtcga tacctggcgt tacgtgacct
      541 ggcagatcta cgacaacatt gccgcgacga cctccccctc ctccggcgaa ggtttacaag
      601 agacgcgcct gaaacaggat gtctactatc tggaaaaacc gcgccgcaaa acggaagcgt
      661 taatctttgg ctctcacgac aactcaacgc ttgagatgac tcaacggatg tccacctatc
      721 tggacacatt gtggggcgca gaaaatgtac cgtggtcgat gtattacctg aatggtcagg
      781 ataacagtct ggtgctgatc tcaaccctgc ccctcaaaga tctcacctcc ggatttaaag
      841 aatcgaccgt cagtgacatt gttgattcac gtcgtgcaga aatgttgcaa caggccaacg
      901 ccctcgatga acgcgaaagt ttttctaaca tgcgccgcct ggcctggcag aacggtcatt
      961 actttacctt gcgtaccacg ttcaaccagc cgggacatct ggcaacggtc gtggcttttg
     1021 atctgccgat taatgatttg atcccaccgg gtatgccgct ggacagtttc cgccttgagc
     1081 cagacgcgac ggcaacggga gacaatgata atgagaaaga agggacggat agcgtcagta
     1141 tccactttaa cagtacgaag attgaaatct cctcggcact caactctacc gatatgcgcc
     1201 tggtctggca ggttccttat ggcaccttat tgctggatac gttgcaaaac attctgctgc
     1261 cactgctgct gaacatcggt ttgctggcgc tggcgttatt tggctatacc acattccgcc
     1321 atttctccag ccgcagtaca gaaagtctac ccagcacggc ggtcaataac gaattgcgca
     1381 ttttacgggc aattaatgaa gagatagtct cactgctgcc gctcggcctg ctggttcacg
     1441 atcaggaatc gaaccgcact gtcataagta acaaaattgc cgatcatttg ctgccgcatt
     1501 tgaatctgca aaacatcacc accatggcgg aacagcatca ggggattatt caggcgacga
     1561 tcaataacga gctgtatgag atccgcatgt tccgcagcca ggtcgcgccg cgcacacaaa
     1621 ttttcattat tcgcgatcag gatcgcgaag tgctggtaaa caagaaactc aagcaggcgc
     1681 agcgtctgta tgagaaaaac cagcaggggc ggatgacctt tatgaaaaac attggcgatg
     1741 cgctgaaaga acccgcacag tccctggcgg agagcgcggc taaactcaac gccccggaaa
     1801 gcaaacaact ggcgaatcag gcagatgtgc tggtgcggct ggttgatgaa atacagttag
     1861 cgaacatgct tgcggatgat agctggaaaa gtgagacggt gctgttctcc gtgcaggatt
     1921 taattgatga agttgtgcct tcagtgttgc ctgccatcaa gcgtaaaggt ctgcaactgc
     1981 tgattaacaa tcatctgaaa gcacacgata tgcgccgcgg cgatcgcgat gccttacgac
     2041 gtattttgct gctactgatg caatatgccg tgacctcaac gcaattggga aaaatcaccc
     2101 ttgaggttga tcaggatgag tcctccgaag accgcctgac gttccgcatt ctggacacgg
     2161 gagaaggcgt aagtattcat gaaatggata atttgcactt cccgtttatc aaccagaccc
     2221 aaaacgatcg ctatggcaag gcggacccgc tggcattctg gctgagcgat caactggcac
     2281 gtaaactggg cggtcattta aacatcaaaa cgcgggatgg gcttggtaca cgctactctg
     2341 tgcatatcaa aatgctcgca gctgacccgg aagttgaaga ggaagaagag cgtttactgg
     2401 atgatgtctg cgtaatggtg gatgttactt cggcagaaat tcggaatatt gtcactcgcc
     2461 agttagaaaa ttggggtgca acctgtatca cacccgatga aagattaatt agtcaagatt
     2521 atgatatctt tttaacggat aatccgtcta atcttactgc ctctggcttg cttttaagcg
     2581 atgatgagtc tggcgtacgg gaaattgggc ctggtcaatt gtgcgtcaac ttcaatatga
     2641 gcaacgctat gcaggaagcg gtcttacaat taattgaagt gcaactggcg caggaagagg
     2701 tgacagaatc gcctctgggc ggagatgaaa atgcgcaact ccatgccagc ggctattatg
     2761 cgctctttgt agacacagta ccggatgatg ttaagaggct gtatactgaa gcagcaacca
     2821 gtgactttgc tgcgttagcc caaacggctc atcgtcttaa aggcgtattt gccatgctaa
     2881 atctggtacc cggcaagcag ttatgtgaaa cgctggaaca tctgattcgt gagaaggatg
     2941 ttccaggaat agaaaaatac atcagcgaca ttgacagtta tgtcaagagc ttgctgtagc
     3001 aaggtagcct attacatgaa caatatgaac gtaattattg ccgatgacca tccgatagtc
     3061 ttgttcggta ttcgcaaatc acttgagcaa attgagtggg tgaatgttgt cggcgaattt
     3121 gaagactcta cagcactgat caacaacctg ccgaaactgg atgcgcatgt gttgattacc
     3181 gatctctcca tgcctggcga taagtacggc gatggcatta ccttaatcaa gtacatcaag
     3241 cgccatttcc caagcctgtc gatcattgtt ctgaccatga acaacaaccc ggcgattctt
     3301 agtgcggtat tggatctgga tatcgaaggg atcgtgctga aacaaggtgc accgaccgat
     3361 ctgccgaaag ctctcgccgc gctgcagaaa gggaagaaat ttaccccgga aagcgtttct
     3421 cgcctgttgg aaaaaatcag tgctggtggt tacggtgaca agcgtctctc gccaaaagag
     3481 agtgaagttc tgcgcctgtt tgcggaaggc ttcctggtga ccgagatcgc taaaaagctg
     3541 aaccgcagta ttaaaaccat cagtagccag aagaaatctg cgatgatgaa gctgggtgtc
     3601 gagaacgata tcgccctgct gaattatctc tcttctgtga ccttaagtcc ggcagataaa
     3661 gactaatcat ctgtaggcca gataagacgc gttagtgtct tatctggcat ttgcaccgat
     3721 tgccggatgc agcgtaaacg ccttatccgg cctacgattc ccattatttc aacagattac
     3781 attaaagtag gccagataag acgcgtcagc gtcgcatctg gcatttgcac tgaatgccag
     3841 atgcggcata aacaccttat ccgtcctacg attcccgcga tttcctgacc ctctcggcat
     3901 ataccgtcag cgtctgtttt atcacatcca gcgttaccgg cttcgacagg cagctgtcca
     3961 taccggactc cagacaccgc tgcttctctt cagccaacgc attagcagtt actccgatta
     4021 ccggcaacgt cagtcccaac tgacgaatgc gttgcgtcaa gcggtaacca tccatatttg
     4081 gcatgttgac gtcgctaagc acgatatcaa tatgattctt gctaagtaca ttaagcgcat
     4141 cgacaccatc attcgcggtt ttacattgat agcccaacga tcccaactga tctgccagca
     4201 gacgtcggtt aatcggatga tcatccacga ccagaatcat catatcgtca ttatcgctga
     4261 ccgctttgtc cgtcgacggc agagcgttag caggatcgtc gctctccatc tcgatcaaat
     4321 aaatacgcgc caacaatgcc ggtagctcat gcggggcagc cacactgtgt acccactccc
     4381 ctggcgcttt ctccagcgga ataccaatat ggcgacgaca gaaggtcact accgctctac
     4441 cctgccattt tttactcact acctcgtcag taattaacac atcttcggga gtcggttcct
     4501 gcccttcgta tgttgtaacg acgatgccgc tgcgctgcaa actggtttcc aggaactgac
     4561 agagcgacgc attgcggacc gccagccagc agcgtttacc actcaaccct tccacgcctt
     4621 ttttctgcgg gtactgagcg ccgtacaacg gaatacgcac ggtaaactgg ctgcccattc
     4681 ccggttctga atctaccgag atatcgccgt ccatcatgct gatcagtttt tcacaaatcg
     4741 ccagacccag accggtcccc tggaagtttc gctgtacgcc cgttccgacc tggaagaagg
     4801 gatcaaacaa gcgcaccact tctttcgccg gaatccccac gccggtatcg cgaacacgga
     4861 tagagagata atcgccatca gcgcggacat gcaaaactat acagccggta tcggtgaatt
     4921 ttatggcgtt actcaacagg ttggagatga cctgctgtaa acgcatcggg tcgccattta
     4981 aggccactgg cacatccggt tcaataaagc agtacaagcc taactgcttg cgtaccacca
     5041 gcggtaaata gttggcggtg atgtggttca tcacttcacg cggtgaaaac tcacgcggtt
     5101 cgatcttcaa ctgttccgat tcaatcttcg agaaatcgag aatatcgctg ataattttca
     5161 acaacaggct ggaagagttg ttcattgccg tcaccagccg atcgacgcct ttcggtaact
     5221 ctttggtttg caacagatcc aggttaccga taatgccata cagcggcgtt cgcagctcat
     5281 gactgacggt ggcaaggaac atcgattttg actggctcgc ctgttccgct gcttgtgcca
     5341 tctcctgcaa cgactcttcc atcttcacgc gcgaagaaac atccaccagc acacaaatgg
     5401 ccacgttttc attacgatag cgcgaatgga cgaagctgat ttgcagattg gtattgttgc
     5461 tggtcaggac atcaacaaaa ttgacctgct gcccacagat aatttgcgtc agtcgttggc
     5521 ggtcctcatg cgtaagcata ttgagatagg tatgcgccag ttcgttactt aaaatattga
     5581 cgccatcagc ggtacgcaaa atgcagatac ccactggcgc ggaggcgaca atcttgcgat
     5641 tgaactgctc atgttcttcc agtcgcaggg cgtcgctttc cgccggaatg aaaatacgtc
     5701 gctcgtacat ccgcgcgaga gtaaacaatg cagctccggc aagcacattc agcaaaattg
     5761 cgttaaggat caacatgcga atgcgttcca gcaccttatc aaccggcacc gaatacacga
     5821 tgcttagcga tgagggtggc agatttttct tcagcaccag ctcccggaac ccttccgtat
     5881 agccaaacca ggagcgttcc tgcatccagc gaggatcgcc cttaatttta ctttctggtc
     5941 cggtaagcga aatcagggta tgaccatttt catcaagaat ggtaaccccc atcggcaacg
     6001 tacccggtaa gaaaaagttc tccatccgga tggtctgctc gacacccaaa agcgcctgca
     6061 accggttcgc cagataaact ggcgtcaacg cgtaaaaata tccgacgcca gggcgcggac
     6121 cttcgctgat ccagtagagg ttactgccgc tatcatcttg tggtgcattt cgatatttat
     6181 tgatgcgttc atgcaaagct ttcaacgcgg tatcgcgttc cactggcata tcacgcagac
     6241 cgaaattggc catgcagagg ttatcactgc cgattaaaaa tacccggttg agatcgtaag
     6301 ccgcagaaaa attatcgcgc cagtagcgca taaaccacgc caatgactcc agagaacctc
     6361 gccaggtgtt actcattgcg gaacaatcgg agtcggcaaa cagcggttca aacgcaggca
     6421 catccgcctg cgtttctcgt ccacgcgggg aaagcacacc gttttctgcc gataagcgat
     6481 tttcggcgat gtacttcagc tctttcatca catcagaggt gcgttgaata aagcgctgag
     6541 cctgatcgga actcagatta aattcctgac gaatttccga ttctcgctga tgtaacgcat
     6601 taacgatgta aaaaacggat gaaaaagcaa tcaacagcca gagcactaac gccaatgctc
     6661 tgaacatgta gcgcgaggct ttcagggttg tacgaaaaga agcaaggtat ttcaaagggg
     6721 cgaagctccg cctcaggtga ccgatggagt gtggttaagg tagcggtaaa agcgtgttac
     6781 cgcaatgttc tgtcaccatt gcaataatgc gtatcgctta gaaaccattg ccttaaccag
     6841 gaaagccgga gtagatggac agttctcagt acgaaaaata agaaatgacc aatctgccga
     6901 cgcttgttaa tggttatatc ctggcgtcgg cagatcgccc agcagtctta tctgaaaaaa
     6961 gagagagcca ggttgtgtga agggatggat caccagtcaa aacttttcac cagcgtcagc
     7021 tcgccagcat tacgcatcgg tacaataaat gtttcctgtt tctcattgac cgatccttca
     7081 tcggtgatca gcgtcagttg ggcggtcgtc aattccgttt cgctgcgccc accatagtag
     7141 ttgatataca cctgatagcg cccgtgaact ggtgctggca tggcgaaaat ctcgggtccg
     7201 taccccgtcg tgacatccat atccagtgca ccactgtttt tcagcacggt gttaccgtac
     7261 caggcgtgtt cgccatcggg cgtgacaaca tggagatcga gatcggtatt gtccgtatcc
     7321 cacgagagaa ccagccgtaa acgtgcacga atcgttcctg tacccggcgt tgagtaaaac
     7381 tgcatttttt gtcggctttg cccatccggg ctgatgacct gcacgctgtt gctgccttca
     7441 gtgaaaatat aagggcgtgc aaatgaacca tcggattcaa ctctctgcgg catactggca
     7501 ccgttgacca ccagccggcc ctgctgaact ttacccgccg cagcataatt ctttattttg
     7561 ccgcggatct gcgctgagat attttgatca tctgccatgt tgacggacga cgccgggtaa
     7621 ttgatcgatt gtgaaaaact ggcatcttcg ccctctgccg gatgccagcc agagagcggt
     7681 gcgtcaatct cgacttcctg cacaccttca ctgtgagcga caggcgaaag tgccaccagt
     7741 aacaacggaa gaaaaatctt tcgcatagca ttagtccatt aataattgtc gggtcagcgt
     7801 ttcgatatag ttctcatcaa gcccggtggg ataaccatca aaggccaggt gcagatactc
     7861 atgggttaaa tccagccgat cctgaagcgt aaagaagtta cgaataaaca gccgtttctg
     7921 ctgccgatcg gtatagggga aacctgagac taaacgacag accgcaaaca cgtctggttc
     7981 gttgtacccc gtctcgcctt gtaatatacg ccgccactgc ggcatttttt tcgccagcca
     8041 ggctttcgct ttgggtaata actggcaggt tgagcgtgga gctccccagc ggctaaggct
     8101 gttgtcggga taagcaaacg cgagtatctg gtcgtaacgc tccccctgcc ccgcctgggc
     8161 cgtggcttgc cgccaggaaa gtgtcccttc ggtagcgcga ctgccatgat aatgaaccgg
     8221 atcgcctgca tagatgagat cctgcgtcca ggcggtcatc gttcgcgccc ccgtggtcgc
     8281 tggcgaagca gaaacacgct gcgtggcgct gctgtcgggg atggtcaggc aatcgccttc
     8341 ccgattggcg ttttgctgta aaaaagtgcg aatcgccacg gtcatggctt tcgctgcctc
     8401 agggggcgta cttttcgcct cgcgatccag aactctggca acgtactctt cacgatcgag
     8461 atgcgactgc aatttcagtt tgcccttttc gctcagtaag gtcgtctcac cgtggctgac
     8521 aaacgtaatg tggttaccat tggtaaacgt cacacgatag cgcccattaa gtacgccggg
     8581 ttttactgcc gtcgtactct tctccgctgt gattttcttc agcggatagc gggcaaacag
     8641 ctccacctct acacattgcc cactggcaac ctgcgtcggc actggcaaaa cccgattaag
     8701 caccgtcgca taacgggtta gtaccgtttt gctggtgcca ctgccagtaa cccacagcgg
     8761 tgtaccatcg gttaaccagc cagcaaaacc accctgacgg atttcctgtt tatcatcggc
     8821 aaaccagctc caggttttca cccgtacccg actaccgagc atggatgcga caccgatttt
     8881 cgcctcgtcc agcaccacat ccagtaagac ttcctgagct ttgttttgcg cgggtaacgt
     8941 cgctaatgaa tcaagcagcg atttaaccgt taccgaggtt tctggcttca gcgtaggcag
     9001 agacgctaac cacgcaggtg cctgacgctg ttgccagtat tgcccccaca catctgcgcc
     9061 aatatgcaac cgctgcggcg caaaatagag cccacaggaa cgtaccagcg cggtatcgcg
     9121 cgttatgctc tccccagcct gacaacaata aacctcttcc ggggagtttc cgcgacactg
     9181 gtagggctgc tccggttgat gggtatcctc cagccaggcg taaacataca gtttccacaa
     9241 actccctaac ggcgtggtga gcgagtcagg taatgctgag actttggtca cgcctgatga
     9301 cgacagctga tacagttgat cgtgctgcgc accgcgcagc accagttgta acggcgtctc
     9361 ctccgcaagc gttggcagag taaccaacgc caatagccag acgattctgc gccagttcac
     9421 ttatttcacc tgcatcccgg tccattcact ccctgccgca acactttgct gcgcaggtgc
     9481 ataggaacgc acataacgag caggaggcag aacgaattgc cctttttgcg agaagcgcag
     9541 caaatggcgg aaagtgaccg ttcccgtcag ttctttcacc ggcaccatat aggccagttc
     9601 gcccatttca ttacgcgctt tttccagcaa ttgcccctgc tgtttcgcgg cgttgggttt
     9661 attgactgaa atgccccatg ttgtgcgctc aacgtcggct cccggcggga gcggtacttc
     9721 cacctgacca tagcgcagaa ctgcatcctg ctcgctggta agcgtaattt catcgagata
     9781 cagcgcatcg ctgtcaatct cattgctggt caccggctgc agagtaaagc tcatctcttc
     9841 ttcaccgggg ataagccgat acaactggcg ttcaacggtc accggaatgt tactttgttg
     9901 agccgttttt gccggttcac gccagcggac ctgcacattt tgcggcgata attcgtcacc
     9961 aaaagagaga atgtccggca caccctgacc aacccaacgc cagtcttcgc cccctccagt
    10021 taacttatgt ttagcccatg cgcccgcagg cgcaggcaaa acaactggag gcattgtcgc
    10081 catatatttc gccagccagt tcatggccag cgcgcgctca atagtggatt gctcagcggt
    10141 caaaccactt aaaatagcgg ccgtctgggt agcgtcacca ccgctattga gcagcacaac
    10201 cgtgtgcgcc agcggctgat tgctgtttgc cgctttatcc cacgcatatt gctgaacctg
    10261 tggcatcacc gtggacttca actgcgcttt tttcagcaac gtcatggtta agacgtttgc
    10321 caccgcatct gccagtggag actccggtgt atcgaggatc aggctatcat tgatatcgcg
    10381 ggtgtcttcc tcagagaaat cttcagtttt agttccgcgc cgggcgatag cttcatccaa
    10441 ccctttcaac aacgttttgc acggcaaatt catctcctgc gcccatgcca gcaccagcgc
    10501 ccgatgcaat aacggcatgt tatctgcctg ctccgcgtag ctgtcgagca tatgctgcca
    10561 gtattccggt tgttgcgtta cgccgatcgc ctggctggcc tgccagtcgg cgtaccatgc
    10621 ccatgccgta aggaaggcgt caccattgcc atcttcaccc caccaggtaa agcgcgctcc
    10681 gggccccgcc agttgcatca gccgcagacg gttatcctga atcatctgac gaatgtcgtt
    10741 agcggcggca ctttgatgat cggcaagcga acgccaggcg agactgagcg ggatcagacg
    10801 gctaccggtg ttgattacgc caccccacgg ttcatcgact aacgcatcaa gattgttgcg
    10861 gaaaatctcc tgcggcgttt cactactttg tagccggata ttgctcgcct gctcgggcaa
    10921 catcagcgcg ttatcgccac caccgagcat gacatttttc tgctgttcaa cgggccagct
    10981 gttatccaca aaagacagtt ttgtgctaat gctgtcctgc acttgcccat tttgttgcag
    11041 ttctgcgctt aacaagccag attgctgaat gttctgcgcc agcgaaatat aattcgcccc
    11101 tttgtgcagc gtcagcgtct ggcgcatctc agcgcctgca aatttagtca ccagcgccac
    11161 cggttcgtta tcctgctgac tgaagataaa cagtcctgcc gccggtttgt cgcccacgcg
    11221 atacaccgtt ggcatactcc acttcatgta gagatttttt tccgaacgca gataagcacg
    11281 cccctgcccg accagcccgt cgccgttcat cccacgcgcg gtgatacgcc agcgggttaa
    11341 cgaatcaggc atcaggaacg tgaaatacgc tttgccttgt ttatcggttg tgagtgacgg
    11401 catccatgcc gcggtatcca cctcttcacg ccgtggacgt tcaagcattt ttactcgccg
    11461 ctcgctgcgg ttagttgcgc caggcgcaac cggctcgctg gagagcgcct ggtcgtagct
    11521 gataaacgac agactggagc tggtacgcac attgttacgc cccagcggat agaaaaattt
    11581 gccgatattc ggcgcgattt ctggttgcag cgcgtagatc atttcatcga ccacgcctac
    11641 cgttagctgc gcagaaacag gtttaccttt cagcgacgag gttaattcga cattgaccag
    11701 ttcaccaggc tggtaatggg ttttgttcgt tttcacccgg atatcaagct gaggaacggc
    11761 aactttgatc ccggcgttct gaaaactgta ctgaccatta cgggtataca gcaccgaaaa
    11821 agtgatgtta ggcgcaaagg aattgctcac tggaacccgg gcttcatact gggtatcgtt
    11881 taaacgttgt aacgtcagcc agtttgccgg atgcgaaagc agcgactgct gttccacgcg
    11941 atcgcgttcc agcgtcaata atgcttcatc aattggctcc ggaaaggtaa tcagcatctt
    12001 cgcagtttcg cctggctggt acagcgtttt atccgccacg atatctaccg taccagtatg
    12061 cgccgtgctg cccttaccgc tgacggcatg acttaaccca gcgagaatta agccgtcttt
    12121 atcgcgtaat gtcagattgt agttgccagg tttagcgaaa ttgacggtaa aggatttgcc
    12181 gcctgacggt agctctccgc tatggctcgt gcggtcttcg agacgcaacc attcatacgt
    12241 aacaggaacc tgttttgaag attccagcgc ggcataacgg aacacaaccg actcgccgct
    12301 attactgtat tgtgcggcag tacttaatga gtaatgcgcc agaccgcgtt caatgaggat
    12361 ctctttggtg gtggtgacac gatacgccgc gccgtcactg gcggagacgg ttaacaaata
    12421 acggctcggt ttatcggcgg cggggagatt taacgccaca tgaccgctgg cgtcggacac
    12481 cgtttcgctg ccttccagcg acacggggaa acgtccggca taacgcaaat cgttaccgac
    12541 cattgataat tgctgagcgc gcaaacttaa ctgcacgcgg gcatttttta ccggctcgcc
    12601 atccgggtag agcagttgca gtttgccgct gaccgcttcg ccagttttga actctttttt
    12661 gtcgagagct aaaccaatct cgaaatgtgg cttgatgtag tttgccacgc gaaaactgct
    12721 gctatagacc tgattgcggt aagcaagacg taactcataa cctccggcaa cggcattttc
    12781 tggcaggcgg aaacttccct gcccgccatt gcgcgcatcc agcgtgacat cgacggtttg
    12841 caacagactg ccgttagcgt ccagcaccga aagcttcgcc ggggcgctga cgatggggga
    12901 tgaatgcaac ggatcgtgga actcgcggcc catcacttta acatcgacac gatcgcctgc
    12961 gcgatatagc gggcgatcgg taaaaatata caagcgggtg ttgtagattt cgctttcgta
    13021 gaagaagttc tcggagacaa aaacgccgcc ttcagcatcc ttacccagaa tgtatgaacg
    13081 ttctggcgat atatgttgta actgcaaggt accgctgtca tcggtcacac cgcgggtcat
    13141 cacgccaaga ccgtcagtcc acaagatctc tgagccgggc ttcgcttcac cctgtttttt
    13201 acccgcggtc cacaccagaa gctctttgcc tgacactttg ctaagcgcca cggtatcgga
    13261 aacaaacacc accgtcgtcg cccgataccc accaaccatc gcctcgacga ggtacagtcc
    13321 cggctcttgt tggccgagag gaatataaat gttacccggc tgcggcgaga tgaaattgct
    13381 ggatgcgcct tccagtttta ccccttgctg cggctcgaac ggtttagcct gccatagtgg
    13441 ataacggaac tgttccacca ggggatattt tttcagcggg gaaaactggt tgttctgtac
    13501 ataacgggaa ggtttaataa tggcattgcc gagctgtaat tcgggtaacg cctgagtcac
    13561 attctgcctt gactgagaag agaaagtacg ctgcatcacg cggcgagatt tgccgtacca
    13621 gttatcccac agccaggtta gcgtattgtt cagcccgtcg cccaaatatt gcggttgcac
    13681 cacaatgcga tgcaggtttt tctgctggcg caaaaatgcc atcgggtcag gaatacgata
    13741 caggcgaacg tccacgccgc cgtactcttc catctgatag cgccgataat cacgccccgg
    13801 cgcttccagt cgcactttcg cctcttcact gctgctaaaa ctgctgtcag caagcaaaaa
    13861 gaatgttccc cctgcgggcg gcgcatagtt actggaagga agcgaatcat cagcattagc
    13921 aagccctgtt cccacaagag acagggacaa gcacgctgct atcgcgccag aaaatttaaa
    13981 cgataaatgc caatgaaatt gggattggaa tcgttgagta tccatcgggt gtccttccat
    14041 gtcataagtt gttgcagact gactgcgcgc attccgttgt cagttttcgt ggcgcttccg
    14101 gtgtggtaga tgacgtaacg ccccatccag accattaagt gctgggcatc gccctgatcg
    14161 aaaaaaatca tatcgccagg cagcgcctgg tttatgtcct ggccaataaa ctggctgttg
    14221 tactgaatca aattaatcgc ggtcacatag ggaccggttt tcccgttccc ctgattccag
    14281 ttttgcgcca gttgacgctg ttcaggtttt aacgtcattt caggcggcaa atactgactt
    14341 gctatgccgt tactttttag ccatttgctg tcgtgaactt tcagcgcctc gttcgctgca
    14401 aatcgcacca ggcccgcaca atcctgctga taccagcgtg gacttggccc ctggcggagc
    14461 tgttcttgtg caatgcgtac aaaccaggcg cgaaacagcc cggattgttc gacattcagc
    14521 atttcgctat gggcaacaac acaacacagc cagcaaatca gcgccagcag cccgtgcctc
    14581 atagcggctg ccaggttatg ggtagccact gccaggcggc accgggttcc atctgggcca
    14641 gcttcatgac ataacgcggt tgttgagata aagcgtccag cttcggcatt aataaagttt
    14701 gtgcggcgtt ataaaaaacc ggttcgagat tcttcggcag actggtcaac gtttcgttac
    14761 gcagcagttt cgctattcct tgtggattga tatagagcgg aacgatgcca tcagtgggta
    14821 ttacatccac cattgccggg cgggttttat tcagtgtttg cagcgcgtta ttaaccagcg
    14881 tgtcatcgag ggagaaaagc agcgttttgt tttgcatcgc cagcgacaca cggaaaaaat
    14941 aatccgacat taattgatcg ggttgcgccg cctgcgcttt cggatactgg ccgtatcggg
    15001 aactcacttc gcgacgccag atttgcgctt cgccctgctg agtctggctt accggcaaaa
    15061 caccttctgg cgctttgctt tcgtgcgcac caatattttg cgtaaacagt ttccctggca
    15121 attgcgccta ttcggcagtg ccatcaaact gaccgacaaa cagcggggtt tgcaattttg
    15181 agtcttcata ccagcacagc cccgcggcac cgtctaacgc cccattcagc ttgtcgtttt
    15241 cctggctgat gtgcgaaagc atctcttcgg caataccgtg tgaatacggc accgccacac
    15301 agaagctggc cccggcaggc atactgttcc agaccggcgt aaaatcgaaa ctggcatcta
    15361 cgctggcgga ttcatcattt aacgccacaa aactgtgcca gccgtcgtta cccatttcga
    15421 agcgtacgcc agcaaaagaa ggcattaatc gctggtagcc aaaacccagc aacctggcgc
    15481 tggccacgat gcgctggcgt actggcgttt tttcggcagc acgctcttcc aggccaaagc
    15541 tggcttgcca gcgttttttg ccgctcaaca aatcacttgc gatcgccgtg gcttcggtat
    15601 cctgctgatc atctttaaac agcatatccg tgctggaaaa caccagcatc ttgtcctgat
    15661 atgtcgcgaa catcagggcg ttattgccgt tatagcgcaa ctgataaaca ggaatagttt
    15721 cactatttat cttgatgcta ctgatttccg ttttgcttaa ctggctgtcg ctggtagcgg
    15781 caaacaacaa tggctccagc agtttgctta aaccactgcg ctggatcagc accatataat
    15841 gtgaaagatg gccttgttta tcgtgccaca gcgctgcctg cgcgggctga tctaagagtg
    15901 acgaaaagag cttatctttc agcgtgagat cgtgttcata gacaatacga cgaatgctgc
    15961 cttcaatgcc cagacgatcg gcatgattct gataatagaa aacgaaatct tcgctcagaa
    16021 catcgtggag aaacggaatg gtgaggagat ctttgggaag ctggctcaga gagtcgctgt
    16081 cgagaaagag gtccggctca ttgagatcga tttgcagatt gttgtgcacc accagcggcg
    16141 acaacgtttt ttctggccca ctgccagcat attgcaacgc ccagacgcca gcggaaagca
    16201 gtgctattgc gccaaaacct acaagaccat agaaccgcca gcctttcgcc tttttttcac
    16261 cactcattgc cacattcctt gtgtatagcc agccattttt tacgggcaca gccaaacttt
    16321 accgtgccct aatacgacaa aagcccagac tttgcagcct ggacttttca attcaaacaa
    16381 gggagatagc tcccttttgg catgaagaag taaaattatt cttcttctgg ctcgtcgtca
    16441 acgtccactt ccggagcgat ttcatcgtcc ccttccgcgg cactgccatc gatggtatcc
    16501 agatcttcct cgtcaaccgg ttcagcaaca cgttgcagac ccactacgtt ttcatcttcc
    16561 gcagtacgga tgaggatcac gccctgggtg ttacggccta cgatgctgat ttccgaaacg
    16621 cgagtacgta ccagcgtacc ggcatcggtg atcatcatga tctggtcgca gtcatctacc
    16681 tgtaccgcgc caaccactaa accattacgt tcagtaacct taatggagat aacccctttc
    16741 gtcgcacgcg acttggttgg gtattccgcc actgcggtac gtttaccgta accgttttgc
    16801 gttgcggtga ggattgcgcc atcgccacga ggcacgatca gagagacgac tttatcgcct
    16861 tcacctaagc gaataccgcg aacaccggtg gtgttgcagc ccatcgcacg gacagaagac
    16921 tctttaaagc gcaccacttt accttcagcg gagaacagca ttacttcgtc ttcgccgctg
    16981 gtcaggtcaa cgccgatcag ctcatcgccg tcaaccagtt tgatcgccac tttaccggcg
    17041 gtacgcagac ggttgaactc ggtgaggaca gttttcttca cggtaccgtt agcggtcgcc
    17101 atgaagactt tcacgccttc ttcaaactcg gtcactggca ggatcgcagt gatacgttcg
    17161 tcctgctcca gcggcagcag gttgacgatc ggacgaccgc gcgcgccacg agtggcttcc
    17221 ggcaactgat aaactttcat cgaatagacg cgaccacggc tggagaagca cagaatatgg
    17281 tcgtgagtgt tcgccaccag cagtcggtcg ataaagtctt cttctttaat acgtgcggca
    17341 gatttacctt tcccgccacg acgctgcgct tcgtattcag aaagcggctg atacttaacg
    17401 tagccctggt gagagagcgt cactaccaca tcttcctgag tgatcagatc ttccaggtta
    17461 atgtctgcgc tgttggcggt gatttcagta cgacgtttgt caccgaactg ttcacgaacc
    17521 agctccagct cttcacggat cacttccatc aaacgatcgg cgctaccaag aatacgcaac
    17581 agttccgcga tctgatccag cagctctttg tattcgtcga gcagtttttc gtgctcaagg
    17641 ccggtcagtt tctgcaaacg cagatccaga atcgcctgag cttgctgttc ggtcaggtag
    17701 tacagaccat cacgcacgcc gaactctggc tccagccatt ccggacgcgc agcatcgtcg
    17761 ccagcacgtt cgagcatcgc ggcaacgttg cccagctgcc acggattagc aaccagcgca
    17821 gttttcgctt ctgcaggcgt cggcgcatga cggatcagtt cgatgatcgg gtcgatgttc
    17881 gccagcgcca cggctaatgc ttcaaggata tgagcacgat cgcgagcttt acgcagttcg
    17941 aaaatagtac gacgggtcac cacttcacgg cggtgacgaa caaacgccgc gatgatgtct
    18001 ttcaagttca tgatcttcgg ctgaccatgg tgcaatgcca ccatgttgat accgaaagaa
    18061 acctgcaact gggtctggga gtagaggttg ttgagcacaa cttcaccgac cgcatcgcgt
    18121 ttcacttcaa tcacgatgcg cataccgtct ttgtcagact cgtcacgcag cgcgctgatg
    18181 ccttccacgc gtttttcttt taccagttcc gcaatcttct cgatcaggcg cgctttgttt
    18241 acctgatacg gaatttcgtg gacgataatg gtttcacgac cggttttggc gtcaacttcc
    18301 acttctgcgc gagcgcggat atacaccttg ccgcgaccgg tacggtaagc ttcttcaata
    18361 ccgcgacgac cgttaatgat tgccgccgtc gggaagtccg gccccgggat gtgttccatc
    18421 agcccttcaa tgctgatgtc ttcatcatcg atatacgcca gacaaccgtt gatgacttcc
    18481 gtcaggttgt gcggcgggat gttggttgcc atacctacgg cgataccgga agaaccgttc
    18541 accagcaggt taggaatttt ggttggcatg acgtcgggaa ttttttccgt gccgtcatag
    18601 ttatcaacga aatcgaccgt ctctttttcg agatcggcca tcagttcatg ggcaattttc
    18661 gccagacgga tttccgtata acgcattgcc gccgcagagt cgccgtcgat ggaaccgaag
    18721 ttaccctgac cgtctaccag catgtaacgc agcgagaatg gctgcgccat acggacgatc
    18781 gtgtcataaa ccgccgagtc accatgggga tggtatttac cgattacgtc accaacgaca
    18841 cgggcagatt ttttataggc tttgttccag tcattgccta gtacgttcat ggcgtaaagt
    18901 acgcgacggt gtaccggctt caggccatct cggacatctg gcagcgcacg gccaacaatg
    18961 accgacatcg cataatccag ataggagctc ttcagctctt cctcaatgtt gaccggtgta
    19021 atttctctcg caaggtcgct catctaaccg ctatccctct actgtatccc ggattcaaag
    19081 gtcgcaaatt ataacacagc cgcgcagttt gaggtaaacc tatacgcttt attcacatcc
    19141 aatgcctgat atactcgttt gtcttgccaa ttacggagta gaagtgccaa tgaatgccga
    19201 aaaatcgccg gaaaaccata acgtagacca cgaagagatc gctaaatttg aagccgtcgc
    19261 ctcccgctgg tgggatcttg aaggtgagtt caaaccgttg caccgcatta acccgctgcg
    19321 tctgggctat attgccgagc gtgctggcgg tttatttggc aaaaaggtgc tcgatgtcgg
    19381 ttgtggcggc ggcattctgg ccgagagtat ggcgcgcgaa ggcgcgacgg tgaccggtct
    19441 ggatatgggc tttgagccat tgcaggtggc aaaactgcac gcgctggaaa gcggcattca
    19501 ggtggattat gtgcaggaaa ccgtggaaga gcacgcggca aaacatgccg ggcagtatga
    19561 tgtggtgacc tgcatggaga tgctggagca cgtccccgat ccgcagtcag tggtcagagc
    19621 ctgtgcgcaa ctggtgaaac caggcggcga tgtctttttc tcgacgctta accgcaacgg
    19681 caagtcatgg ctgatggcgg tggttggcgc ggaatatatt ttgcgcatgg tgcccaaagg
    19741 cacgcatgat gtgaagaagt ttattaaacc ggcagaactg cttggctggg tagatcagac
    19801 aagccttaaa gagcgacata taaccgggct acattacaac ccgctcacta atacttttaa
    19861 actcggcccc ggcgtggatg tgaactatat gctgcacacg cagaataagt gaggttgttg
    19921 tttggccgag ccaatgcctg atgcgacgct tgccgcgtct tatcaggcct acaaatgctc
    19981 cccgtaggcc ggataaggcg tttacgccgc atccggcaac cgtgccgact agacagtgat
    20041 taccatttca ccgtcatcga caaaaaccct gccatctggg caaaatcatc actccctttc
    20101 tgccacgcca cgctgccgcg cagggacact cgctgactga tattgcccgt gactcccact
    20161 tttatttcac cccgttgctt caccgcatcg tcactgatag tgctgccatc ctcttcaatt
    20221 tccgtcgaat ggggattgtg ataataattc agatctaatg ttggtatgac atgaacagcg
    20281 gtacgccatt cgctgtgtaa acccagccgc atctgaatat catcaccctg cgattgtgac
    20341 acgcgcgcac ggttagcggc ggtaaaatca tcctgctgca cgccctgata aatcacctgc
    20401 gcctgcggtt caatcaccac accacgcccc ggtaaccact gatacccggc ttccagcgag
    20461 gcgataatcc ccgacgagtg gtaatgatct gtgccatcgt cttgttcgga aacatcattg
    20521 ttaaaccacg cgtattgcag ccagctatcc agccaggccc cctgcttctg attaccgtgc
    20581 tgataccagc ttgaggtcag cccaacggca taaccgtggt tctggttatc ggcgcgagtt
    20641 ccggtcatat tcgagcggct ctcgccctga ttatcactgt agccaccaac cgccccaagc
    20701 atccactcgc catcatcgcc ccagcgcccg ctaaacaggt caccgctaag ctgcaccgta
    20761 gaagtgtctt catgctgagc cagttgccct gccgccgtgt aatgataacg tccgccgata
    20821 acacgtaaat tcagcgtctg accattgcca cctgcatgat cgcgtcgctc catcataaac
    20881 gcctgatttg ccgcccgcag attattgaga taaccgccaa ctttggcatt cagcaccggc
    20941 tggtaagcgg ggattggatc gggtgttggg tcaggatcgg gtgttgggtc agggtctgga
    21001 tcaggtggca atggtggcgt tacttcttgc gatcgcagat accagtcgtt gttatcttcc
    21061 accagcgtgt agtcatatgc tcccatattg acgtagccgc tgcctgccag actgaacttc
    21121 gcattgtttt gaaactgcgt gggatcggct gcgaaatcaa ccactttaat gcctgtcgat
    21181 gtcggctcac caatccctgt aatggagtta accaccacag ttgtgttgcc agcagtatta
    21241 ccgttcaaca ccaattgatc gcttaccgag tcatcgccgt ttaattcgct atcgagcagt
    21301 agcgtaccgc cgccggtata atcaccgttt accgtcagcg tatcgccagc gacgccattt
    21361 tgcaggctaa ccgcaccgga cgtattggtc agattgccat taaccgtcac gtcggaaaga
    21421 taggtcaggc tgtcagtttc aaggttggta ttaagctcac ctgtcaacgt cacgccatcg
    21481 gtggcattca ccagcgtccc atcgccgtta aacaatgagg cattaagggc gacggaagta
    21541 tcctgcccgg tcagacgcaa aaccgtacca tcgctgatat cgatagtgcc gctggaagta
    21601 gcatcgatag actgaatatc ctgatccgcg ccagtaacga acgtcgcccc atcaccgacc
    21661 cacagtgacg aagcataagg aaagatatcc gcaacatcgc ctttcagggt gccttcttcg
    21721 acacgtactg ccgactgcgt tgtaccgctg gcggtcagct ccagcgtacc cgcccccgtt
    21781 ttagtaaatg tgctaccctc atcctgatgc tgcccgctgc tgtcagccat cagtgcgccc
    21841 cactgcgtgt ctacccccgc atcaactgcc acttcaccgt cggcgcgcat ttcaatatca
    21901 cgcccatgct cagtcgtcat agagcgagta tcgacattgg cagtaatctc catcacgctt
    21961 tcttgttgct tatcggtaaa gataacctgg cggttatagt gggtatctcc aagctgcgag
    22021 ttgtcgctca ccatcagcgt accggaggcg atttgtgttg tgcccaaata actattattg
    22081 tttgccagac ttacctgccc gtcgctcgca tcgaccacca cgtagacgcc atctttcccg
    22141 tcgccgccga tatgcaaatt gtcgccacct gcaagatcta ccacatcctg actgccgata
    22201 accgttccgc tgctgccact gacttcaagg ccatcattgt atgatgtgcc gctctgccag
    22261 gtggagaaat cagagagatc gagcacaccg ccgtttaaca cgatcgactg cggatcgtcc
    22321 tggagagcgg cgagatctgc cgcgtcgcct tccagcgaaa gcaccgcacc atcatcaacg
    22381 actatatcgc cggttagcgc catcgactgc gcccccgaca gcacgtagct gccgttttgc
    22441 gcaatggtga gctgaccagc accttcgatg gtgccagcaa aactgccttg attaacagta
    22501 acattgccac cagcatcgat atttaaagtg ccattctgaa agcccgtcaa tgagtgcaca
    22561 aaggtttgtt gggtggagcc aacgtttagc tctgcctgat tctggtactt atcaatactc
    22621 cctatcgtca gaccgtagca gtcttgcgga tcgtcctggc aatgcgtatc gccgacattc
    22681 atcagggagt tgctgcggcc cagggtaact tcaccgtttt caatctgcat ctcaccagta
    22741 aagtcattgt tatctgcatt aagtaccaga tcgccggaac ctgttttggt gatcaacccg
    22801 gtaccagcaa tagagtcaac agctccgtca ttctctgtat tgccaataac cagcgttttt
    22861 ccgtcggcaa tatcaaaggt aacttcactt aagccgagat acataaagcc acccgccgca
    22921 gtggaaggac catctccata gcctgctgcg ctgttgttct catccactaa cacgccgccg
    22981 ttctggctgt agctgtcatc aacagaaata tcaataagat agggagccgt cgcgctattg
    23041 gtatatatcg ccccgccata accttcggca gtgttatttg taaaggcagt gttatttatt
    23101 atcgtataac ctgaaggatg cttgctgacg ctattattat cggtaacatc gattgccccg
    23161 ccatcgccat cactgtaact tgttgatgta tatgcctggt tgttatcaaa aataacatca
    23221 cttaaataga cgtcattatt aatggtataa attgcgccac ctttgccatc attagcgata
    23281 ttattgcgaa acatggcgtt agtgacacgt aaatcgacgg caccagtatc attagtacca
    23341 gaagaataga ttgcgccacc atagccgcct gcgacgttac cagaaaaaat aacatcagta
    23401 agattcagcg ttgagttttc tttagcaaat atggccccgc cgttattata ttctcctgta
    23461 acggtgttat tggcaaacag agtcattcca gtttcatttt ctggtaatag tgaaaattcc
    23521 gctccttgtt gcaaaaatac ggctcctccg ctggcgttat tggtcatatc cgaaaaaacc
    23581 agccattgcc catcggcgac actccagtcc tgcgtaatgc ctgaaaggct ttgcctgctg
    23641 gcgtgacaac tcgctttgat atcatatccc tggcataaat caatggccgc cgcgaaaccg
    23701 ttagcagaga aaagagatgc aatcattgac gggagtagag ataaatactc cttgcgtaga
    23761 aagataatcc gcatattcat ctaaccatca ttttatataa gacggcatat ttaaacgcat
    23821 tatacaatat gggtta
//
LOCUS       DAPGWD010000061        22640 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPGWD010000061 DAPGWD010000000
VERSION     DAPGWD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.061"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(38..418)
                     /locus_tag="RHX88_002346"
                     /pseudo
     CDS             complement(38..418)
                     /locus_tag="RHX88_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(836..2012)
                     /locus_tag="RHX88_002347"
                     /pseudo
     CDS             complement(836..2012)
                     /locus_tag="RHX88_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2081..2312
                     /locus_tag="RHX88_002348"
                     /pseudo
     CDS             2081..2312
                     /locus_tag="RHX88_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2553..3224)
                     /locus_tag="RHX88_002349"
     CDS             complement(2553..3224)
                     /locus_tag="RHX88_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDV2231033.1"
                     /translation="MLIPEIKQERLYQKIANLIIKLINDNVFPPGSFLPPERELAKQL
                     GVSRSSLREALIVLEISGWIIIQSGNGIIVSDNKHSPVDYTIEEILYVRDLVDSHCAR
                     LAALANNETIINKIELLHFRMENSIHENNVAEFYMLDKQFHLTISEASHNRVLLDMSR
                     MLWEQRVNIPYAGLDKRSGDKNVLIKLNQQHKAIIEAIRAGNADNALQRALLHLKYVR
                     EIIGY"
     gene            complement(3272..3631)
                     /locus_tag="RHX88_002350"
     CDS             complement(3272..3631)
                     /locus_tag="RHX88_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231034.1"
                     /translation="MICEHILISAYVFLYGAEGFIILFVETELSHAKIILENLRNKMA
                     GLRIQALVSERFSVTDNIGVDEYDFYPDYNLLIEREGVLLRQKSGSKPDRRLSATDDK
                     LLIFRHCHAKPVDPNQV"
     gene            3953..4235
                     /locus_tag="RHX88_002351"
                     /pseudo
     CDS             3953..4235
                     /locus_tag="RHX88_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            4390..4677
                     /locus_tag="RHX88_002352"
     CDS             4390..4677
                     /locus_tag="RHX88_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV2231035.1"
                     /translation="MTPAQRRQHYDAWRVSGMSRTAYARQHGINNKTFWHLCRAFSAA
                     DARGPAPADNRPAILPVTLSVSDTATLKLQCACVTASPAGIAAIIRELNLC"
     gene            4671..5009
                     /gene="tnpB"
                     /locus_tag="RHX88_002353"
     CDS             4671..5009
                     /gene="tnpB"
                     /locus_tag="RHX88_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2231036.1"
                     /translation="MLSPHSLWLVREPADIRAGIDSLTRLATQAAGHPPREGEAFLFT
                     GKKRTRMKLLMWDRHGVWLCTRRLHQGAFRWPRDGDTTWSLTAEQFAWLTAGVDWLRL
                     SASPLQRWTE"
     gene            5084..6759
                     /locus_tag="RHX88_002354"
                     /pseudo
     CDS             5084..6759
                     /locus_tag="RHX88_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837254.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6923..7051)
                     /locus_tag="RHX88_002355"
                     /pseudo
     CDS             complement(6923..7051)
                     /locus_tag="RHX88_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7059..7726)
                     /locus_tag="RHX88_002356"
                     /pseudo
     CDS             complement(7059..7726)
                     /locus_tag="RHX88_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7869..8331)
                     /locus_tag="RHX88_002357"
                     /pseudo
     CDS             complement(7869..8331)
                     /locus_tag="RHX88_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8480..9031
                     /locus_tag="RHX88_002358"
                     /pseudo
     CDS             8480..9031
                     /locus_tag="RHX88_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9035..9364
                     /locus_tag="RHX88_002359"
                     /pseudo
     CDS             9035..9364
                     /locus_tag="RHX88_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S16"
     gene            complement(9629..10054)
                     /locus_tag="RHX88_002360"
     CDS             complement(9629..10054)
                     /locus_tag="RHX88_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231037.1"
                     /translation="MNGLKTGLGITPGEHIISADSALSRNIRHCFCLSCRGRLILQTD
                     AQGAWFEHDLHALTAQQKTDCVVLNPEKSHPYIDDMAMFLSPLPVVLEWHCVMCGQHF
                     PGKKFCEACGTGIYSRAVCTKYVYSYQPDLFLDCGSAST"
     gene            complement(10051..10434)
                     /locus_tag="RHX88_002361"
     CDS             complement(10051..10434)
                     /locus_tag="RHX88_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231038.1"
                     /translation="MYAKSFLALDGNRRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTAHGQQCPYVRPERREILLIKRLQQWVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCHTGHFSEEAVAG"
     gene            complement(10600..11169)
                     /locus_tag="RHX88_002362"
     CDS             complement(10600..11169)
                     /locus_tag="RHX88_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV2231039.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKRREGKRVHPTTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGCYATLAHFPDTPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHGTGERNFGCSRS"
     gene            complement(11804..11953)
                     /locus_tag="RHX88_002363"
     CDS             complement(11804..11953)
                     /locus_tag="RHX88_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002208622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDV2231040.1"
                     /translation="MEKVISHTRYNLTSAELEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
     gene            12740..12946
                     /locus_tag="RHX88_002364"
     CDS             12740..12946
                     /locus_tag="RHX88_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV2231041.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            13034..13642
                     /locus_tag="RHX88_002365"
     CDS             13034..13642
                     /locus_tag="RHX88_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231042.1"
                     /translation="MKMKPQYQTRYELLHESYQKWLTGFTRHAVSWGMCHPNIHYFHN
                     LTPGWVSFNGEKPEIAIVPRSLHRLIYGSDKRATPPLDDDLVVNLCTSEHLLVHHPML
                     EGILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTENLK
                     RKSEKELEEWIIDRQKQNTALTDLMDQYVLLAYRTTVDDNRN"
     gene            14840..15757
                     /locus_tag="RHX88_002366"
     CDS             14840..15757
                     /locus_tag="RHX88_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231043.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLIPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRTQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            15842..16714
                     /locus_tag="RHX88_002367"
     CDS             15842..16714
                     /locus_tag="RHX88_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV2231044.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMIITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTDAVFRLFRPVHPVVAVSACTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            17086..19932
                     /gene="ag43"
                     /locus_tag="RHX88_002368"
     CDS             17086..19932
                     /gene="ag43"
                     /locus_tag="RHX88_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV2231045.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKCAGVAVALSLTA
                     VTSVQALAADTIVQAGETVNGGTLANHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGTANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATVTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLMLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIYFDNQTTPDAVLSRAVAKGNAPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVSGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSAK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            20260..21078
                     /locus_tag="RHX88_002369"
     CDS             20260..21078
                     /locus_tag="RHX88_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV2231046.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPSRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRTIHSIDTDIKLNRALWVMAETLLESMR"
     gene            21170..21655
                     /locus_tag="RHX88_002370"
     CDS             21170..21655
                     /locus_tag="RHX88_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2231047.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRMENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            21671..22147
                     /locus_tag="RHX88_002371"
     CDS             21671..22147
                     /locus_tag="RHX88_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV2231048.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            22210..22431
                     /locus_tag="RHX88_002372"
     CDS             22210..22431
                     /locus_tag="RHX88_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV2231049.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            22505..>22640
                     /gene="cbtA"
                     /locus_tag="RHX88_002373"
     CDS             22505..>22640
                     /gene="cbtA"
                     /locus_tag="RHX88_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDV2231050.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     A"
BASE COUNT         5352 a         5984 c         5851 g         5453 t
ORIGIN      
        1 tgttcttgcc cccttatttc cagacagggg ttgtatctta agttaacatt acccgctgac
       61 gtcgatattc tcgcggagag cgataaccca acgcactatg cggatggttt tcattgtaat
      121 gctcgatcgc cactgcaagg ttatgcaatg ccgttcttac attcggtttc ggcatgaacg
      181 cgatgtagtc ttccttcatc gttttcacga accgttctgc cattccatta ctctgagggc
      241 tactgattgc cgttgtgcag ggcttcagat tcagctctct ggcgaactgc cgtgtttcat
      301 gcgctctgta cgcttaaccg ttatctgtca gccactggat ggactgttcc ggtactttat
      361 cgccaaagcg tttttctatc gctcctaaca tcacatcctg cactgtcgct ttatcgtaac
      421 tccgaaccac acggcaaaca attctgagag ttagagcgaa acagcaaatg ttgctgtata
      481 aaaaatagtc ttagcgtggg aaaatttccg aattccaagc tgccccctac gctggaaacg
      541 ccgaaatttc cgatacagcg aatttgttga tctttgaagc atgtttggca ggttttaacg
      601 tgtacgttta tggtgcagta agtgagaatt tattcgtacc agctatggat acgttacggg
      661 cagtgatttt tcgcgccagg cagctatttc tttgctgtcc ggttaacggc ggtcttacgt
      721 acaggttgcc gagcgcaaaa tttgtgcatt ccggaaattt cggtggttgc gacttggtac
      781 cccttttatt gttacaggag gactgggctt gtctgctacc ggtcaggtgt agtggtcaag
      841 taatattggc cacggtttta cagtaaatac ggtatctgtt ctctgattct tccggcgtca
      901 acccaccgtt gtaatggtga ggcctgacgt tattgtaata attcaggata taaccgctaa
      961 tttgttgccg ggcctcgtcc ttgcctacgt aaccatccgt cggcacccat tctgttttca
     1021 gactgcggaa gaagcgttcc attggactgt tatcccagca gtttccccgt cgactgacac
     1081 tttgctttat cctgtaacgc cagagaagtt gttgatattt cagtcctgta tattgacttc
     1141 cctggtcgct atggaacatg acgtcccgcg gctgaccacg cacctcatac gccatccgca
     1201 gggcactgct tatcagggca gtatcggtat tcgctgacag gctccagccg ataaccctgc
     1261 gggcaaaaag atccatgacg accgccagat agcaccagcg attttctgcc cagatatacg
     1321 taatatctcc gcaccatacc ctatctggct cgggcacagc gaactggcgc tcaagcagat
     1381 tcggcaggca ggtatgttcc tgacgagcat ttttgtactg atgttttccg ggctgacaac
     1441 tgctcaggtt cagatatttc atcagacgcc cggcacggta acggctcatc gggacgccgt
     1501 tttgggtcag catttcagcc agcgtgcgcg ccccgcagag cccctacttt ggttccacgc
     1561 ccggcgtatt tcgctgcaca acctgactcg cgccggatta accgtatcgc gtcgttttcg
     1621 ccagtaccgg taactgctgc ggtgtatttc cagagcagaa cagaggctga caactgagtg
     1681 gctgtcactc agtctggcaa ctatcgtgaa ccgttcagcg agtcggacat caagagcgcg
     1741 gtagcctttt ttaatatcgt attttgttcc tccaggcggc gaacctgctt ttccagttcg
     1801 cggatacgtt gctggtctgg agtaataggt gtggcggagg gcgcaatccc ctgacgctct
     1861 cgcctgagct ggcgcaccca actctcaagc gtggtagaac cgacattcat cgcttcactg
     1921 gcttgtcgat atgagtagcc cttatcaaca attagctgtg cacattccag cctgaattca
     1981 ggggtgaaag tacgtttggt tttcttgttc attaagtcac ctgttttgtg ttgtggtgag
     2041 gatatcacct ttaatcaggt ggccaaattt actgtgccac tacaatgtgc atgcaagagt
     2101 gcccaccgat atcacccctg cagcgtatcg gtgaactgta tgccatcgag gcagaagtcc
     2161 ggggatgtac agcagaacag agtctggcgg caagaaaagc cagagctgcg ccactgatgc
     2221 agtcactgta tgactggata cagactcaga tgaaaacact gtcgcgtcac tcggatacgg
     2281 caaaagcgtt cgcatacctg ctgttacagt acctgattcg gcaaggcaac gagagatgag
     2341 ttcggggtcg aggtaatccc ccagagaagt cagtgggtta cgcagagaat cgtaacggga
     2401 taccagatca agggcctgtc ccatgtgcat aaaaaatccg gaaacgagtg agcgtttccg
     2461 gattcttaca cagccactgg atcggtcaac tgatccttaa ctgatcggca ttacggttat
     2521 accggaggta tatttattca gcgcagaaaa ggttaataac caattatttc tcgcacatac
     2581 ttcaaatgta gcagtgcccg ttgcaaggca ttgtctgcat ttcctgcacg aatcgcctca
     2641 ataattgctt tatgctgttg attaagtttg ataagtacat tcttatcgcc ggaacgttta
     2701 tccagcccag cataaggtat attgacacgc tgctcccaaa gcatccgtga catatctaac
     2761 aacaccctat tatggctggc ttcagaaatg gtcagatgaa actgcttatc aagcatataa
     2821 aattcagcca cattattttc atgaatggaa ttttccattc ggaaatggag taactcaatt
     2881 ttattaatta ttgtttcatt attagccagt gctgcaagtc gggcacaatg actatcaact
     2941 aaatcacgga cataaagaat ttcttcgata gtgtaatcga ctggagagtg tttattatca
     3001 ctgacaataa tcccattacc agactgaata ataatccagc cagatatctc aagaacaata
     3061 agagcttctc tcagcgatga acgactaact cccagttgtt tcgccagctc acgctctgga
     3121 ggtaaaaaac tacctggcgg aaagacatta tcattgatga gcttaataat aagattcgct
     3181 atcttttgat agagcctttc ctgtttaatt tctggaatta acatgtgcct cccgctcgct
     3241 tttatcgtta gttatctgac gaattatcat atcatacttg gttcggatct actggtttcg
     3301 cgtgacaatg acgaaaaatc agcagtttgt catcagtagc agaaagacgt ctatccggtt
     3361 tcgatcctga cttttggcgt aataaaacgc cctccctttc tatcaatagg ttatagtcag
     3421 gataaaaatc atattcgtcg acgccaatat tgtccgtgac cgaaaacctt tcactgacta
     3481 aggcttggat acgcaaacca gccattttgt tacgtagatt ttcgagaatg attttagcgt
     3541 gtgataattc tgtttcgaca aacaaaataa taaatccttc ggcaccatag aggaaaacat
     3601 aagcactgat gagaatatgc tcacaaatca tcgaagagaa atttttctgt gtattaccct
     3661 cggaattatg tctataacga ttattaattt tcttgaggat aatcaatatc cagcatggca
     3721 agggagaatt gtttacgtga gtgcaacgcg atagagattt tccgccgcat aatcgtcaga
     3781 ataaagcgtc gatttaatag ttatgtaaga gaatctttac cgtttccatg aatgtaccta
     3841 aggtcattag ttcttttctg acagcttccg gaaaattttt atgtagagca gaaggcgtaa
     3901 caagtctatc ctccctccgc ttctttcatt ttctggtatt gtgccgggga tacgcgtggc
     3961 aatttccgtg gccatgggga ggcatcttac tatacctacg ctgaagcgtg tgaaggtcag
     4021 ggtatgatga catggttaaa tactcacggc cactgcctga cctacttgag caacgtaccg
     4081 aagctgctca gccccgataa actaagaagc gcagcgctat gttcctgtca tccacgacag
     4141 taaccaggcc ctgcagagta ctatggtacg gttatcatac cggttcaccc acgcaaacca
     4201 aaagcacaaa aaacggtgtt ttcaccgttg aaagatagct gcttgcccgt atccataatg
     4261 aaaccttcca tgcactcagg acgttgaatg gtaagcatcc ggtgaactcg cattgccggc
     4321 cgttgtgaac cacgccattc tgcaggttct tcctttatat gcaaaagaga gaaaactgat
     4381 ggcaaaaaaa tgaccccggc gcagcgacgc cagcactacg acgcctggcg cgtcagcggc
     4441 atgtcccgga ccgcgtatgc ccggcaacac ggcatcaaca acaaaacttt ctggcatctc
     4501 tgccgcgcct tctccgctgc cgacgcccgt ggccccgctc cggctgataa cagaccagcc
     4561 atcctgccgg tcaccctctc cgtcagtgac actgccaccc tgaaacttca gtgcgcctgc
     4621 gtcaccgcct ctccggccgg catcgccgcc atcatccggg agcttaacct gtgctgagcc
     4681 ctcattccct ctggctcgtc cgcgagcctg ctgacatacg cgccggcatc gactccctga
     4741 cgcgactcgc cactcaggcc gccgggcatc cgccccggga aggcgaagcc ttcctcttca
     4801 ccgggaaaaa acgcacccgc atgaaactcc tgatgtggga ccgccacggc gtctggctct
     4861 gcacccgccg cctgcaccag ggcgccttcc gctggccccg cgacggcgac accacctggt
     4921 cgctcacggc ggaacagttc gcctggctta ccgccggcgt tgactggctg cgtctctctg
     4981 ccagccccct gcagaggtgg actgaataac ctcctgtgcc agaatcataa tacgggcagc
     5041 ttcttatcag gatgttcccg cccgcctctg tcatcatggc tgcatgacag atgacatcct
     5101 gaacaccaca caaaaccccg atgaactgcg ccgtctggca acagcgctgc tgacggcaca
     5161 ggcatgcgaa tatgagcagc gccttcataa cctgaatacc gtcaacacca cgctggaaca
     5221 gcgcctccat aacctgaata ccgtcaccac cacgctggaa cagcgtatcc atgaccttaa
     5281 tgccgccatg caggcagaaa aaaccgcata tgaacagcgc atccgggagc tggaggaggc
     5341 cctgaagctg gcccaccagt ggcgcttcgg gcgtaaaagc gagcgcctcc cggccagcca
     5401 gaaaccactg gcagatgagg acgcggccag cgatgaggct gatatcaccc ggcaactgag
     5461 cgacctgctg ccggtgaagg agaaaaccgg gaagaagccg gcccgtcacc ccctgccggc
     5521 acacctgccg cgacaggaaa ccgtactcat gcctgagacc ggtagcacct gtccggactg
     5581 cggaagtgaa atgcgacata tccgcgacga ggtgaatgag gtgctggagt atgtaccggc
     5641 acacttcgtg gtgaaacgga ccgtgagacc gcagtacagt tgcccgtgct gcgacacggt
     5701 gcacagtgcc gtgctgccgc cggcagtcat cgacaaaggg cagccgggtc cgggtctgct
     5761 ggcgcaggtg gtgaccgcga aggtgctgga tcacctgtca ctgcagcggc agcagaagat
     5821 atacgcccgt gaaggggtac agttgccgga aagcacgctg acggactggt tcgggccgac
     5881 ggcggcggtg ctgtcgccgc tggcggcagc cctgaaacgt gacctgctca ggcaaccggt
     5941 gctgcaggcg gacgagacgc cgctgcagat actggatacg cggaaaggga aagtccggaa
     6001 agggtacctg tgagcatacg tgagtgcggc gggcagtgcc cgggacatcg tggtgtacga
     6061 ctgccgtccg gggcgtgcgg gcagtatgcg tgtgagatgc tgagcgggtg gtcggggact
     6121 ctggtggctg acggttatgc cggttaccgg gcgctgttcc gtgacgggca ggaaggggcc
     6181 cctccggcgg caccgggtat ctgtgaggcg gggtgcatgg cgcacgtgcg cagaaagttc
     6241 atggagctgt acagaatgaa cggcagtccg ggggcgaagg aggcgctgaa acagatacgg
     6301 gcgctgtata tcctggagca gaccatccgg cagcgtccgg cagagcagaa atgacgatgg
     6361 cgacggcggt acgcgaagcc gcagatggag gcgttccaca gctggctgag ggcgacggaa
     6421 aagacgagcg cgccgggtgg caggctgcac ggtgcggtga cgtatgccct gaagcgttgg
     6481 ccggcgctgg aaacatacct gaatgacgga cgggtaccgc tggacaacaa ccggagtgag
     6541 tagatgatgc gtccggtggc gcaggggcgg aagtcatggc tgttcgcggg ttcgctgcgg
     6601 ggaggagagc ggctggcgga gctgctgacg ctgctgcaca cggcgaggct gaacggtctg
     6661 gagccagtag tctggctgcg tgatgtgctg gagaagctgc cgtcatggcc ggcgtcccgg
     6721 ctggatgaac tgctgcctta ccgccatccg gcggactgaa tacgccctgc cataacgccg
     6781 gtgatcgcgc catgcgagtt caccggatgc ttacgttgaa tgtacgtttt tgggatttac
     6841 tgacggatat gaataaccgg cccatgaagg agtacggcaa aaccagcgca gaacacttcc
     6901 gtatgcgctc atttggggcg ggatttgcac agtattcagc ttccagaatc tgtacacttt
     6961 tccactcaca cagcttcctc aaaatacagc ctattgctct acgcttctct ctgtagaaca
     7021 cctgtcttcg gtattttggc gcaaaaacta tgtgatattt acagttccaa gcggctcgtt
     7081 gccattcgcc agtccttcaa cggaatcgtc catttctggg acgcagactg gattgccagc
     7141 cacaccactt ttttcaccga gtcgtctgtc gggaacactt tacgcttttt gatcgcatgg
     7201 cggatcacgc tgtttagcga ctcgatggca ttcgtcgtat agatcacttt gcggatgtcc
     7261 gttggataag cgaagaacgt ggcaagattc ggccagttag cctgccagct tcggctgtca
     7321 gcgccggtga aacatctgcg tcatacagtt ctttgaacgc agcagctatc tcacgagtgg
     7381 tcatcccttt ggcatacaac gagaggatct ggttatccat cccggtaata cgggtctgat
     7441 ttttctttac cagttgtggt tcgaaggtac cgtcacgatc gcgcggagta cgcagttcca
     7501 gtggaccgtc gcctgtgata acggtctttg tggaataacc gttgcgggag ttagctccta
     7561 gtctgggctg atttttctca tacccaagat ggtgtgtcat ctcagcattg agagcggctt
     7621 caacgctgag ctttttcagc agccgatcaa actgactgag gtcttcagag gttttgaggt
     7681 ttttggccag ttcgttagcc agagcctgta actgtttttc atccataaat taaccttcat
     7741 atgatgctgg attgaacata tcaaaatcag gcgattacac aaatctatgt acaggttcag
     7801 ccgctttatt atcgagttcg gtgaccgcca ggacggtcac ttctgagaaa aggcatttac
     7861 acagaatctt aaacaggttc acgcagcact ggcgtcaggg cattgattgt cgctttcact
     7921 gatttgctcc ggtttcccgt ggtcagactg cgcactttct ccttcagcct gttcacactc
     7981 gtcagggcta ttttcagctt caactgtttg tgccatgtca tactgtaccc gaggaacttc
     8041 tgctcccacg ggcgcgccac cgcgctcttc ttctcattca ctttctgctt cagtttgttc
     8101 tctacgacgt cctgatacca tgcagcaatg gccgccctgc ggcatgcctt cctgccattt
     8161 ctctctttgc ccgtcccgga ctatttcctc tttcaagtag cggcgtatca gcctcagcac
     8221 ccgcctgtcc tttatcgtac tggccagacg cgtcatcagg agtctgtgat ccacacggtc
     8281 gaagaacttc tccagattta tatccaccac ccaccgtttg ctgccccgta tataatgctg
     8341 tgcctattgc actgcctgcc atacgttgcg tcccagccgg aaaccgtgcc tagagtctga
     8401 gaagcttttg tccacgatgg cgcttaaatg ttgcgctata gctatagcct gctggatagg
     8461 cctatccacc actgtcggga tccctaacta ccttgaaagg cagttcgccg tgaccgagcc
     8521 aaatcaggtg tggtgcggtg atgtgaccta tatctggacg ggtaagcgct gggcgtacct
     8581 cgccgttgtt ctcgacctgt tcgcaagaaa accagtgggc tgggccatgt cgttctcgcc
     8641 ggacagcagg ctcaccatga aagcgctgga aatggcatgg gaaacccgtg gtaagcccgg
     8701 cggggtgatg ttccacagcg atcagggcag tcattatacg agcaggcagt tccgacagtt
     8761 attgtggcga taccagatca gacagggtat gagccggcgc ggaaactgca gggataacag
     8821 cccaatagaa cgcttcttca ggagtctgaa gaacgaatgg atgccggtgg tgggttacgt
     8881 aagcttcagc gaggcagctc acgccataac ggactatatc gttggatatt acagcgcact
     8941 aagaccgcac gaatataacg gtgggttacc cccaaacgaa tcggaaaatc gatactggaa
     9001 aaactctaac tcggtggcca gtttttgttg accacttcat gtcgttgaac tgcataatac
     9061 tggcccaagc accgcaacca gtcttgctgc gcttatcgct ttatgttcga tattgctggc
     9121 aaaaccagta caggaacaga tggtggtgtt gggcagtatg acgcttggtg gagtaattaa
     9181 cccggtacaa gatcttgctg ccagtttaca gctcgccttc gacagcggcg caaaacgagt
     9241 tctgttgccg atgtcctcgg ctatggatat tccaacggtt ccggcagagt tatttaccaa
     9301 gtttcaggtg agtttttact cagacccggt tgatgctgtt tataaggcgc tgggtgtgaa
     9361 ttaatgtagt aactatttta atgaactgga gaggacaatg gctcaagaac gttctgtaat
     9421 taaattcatc agaccttgcc tctccattca tgaatataaa aatacttaca tctgagtatt
     9481 gttggctgtg tttgaataaa aagagagctg ttcaaataag cctgtataac tgttctttac
     9541 aaaaaatgga ggagctatca gctaattcaa cactggaaga tgttcgtcga gttggattga
     9601 acgccctgcg cagaccaggc atttcggatt aagtgctcgc acttccacag tccaggaaca
     9661 agtctggctg gtagctgtaa acatactttg tacataccgc tctggaatag atccccgtgc
     9721 cacaggcttc acagaacttt ttcccgggaa aatgctgccc gcacatcaca caatgccact
     9781 ccagcacgac aggtaatggc gataaaaaca tcgccatatc atcaatgtaa ggatgggatt
     9841 tttccggatt cagcaccacg cagtccgtct tctgttgcgc ggtcagggca tgtaaatcat
     9901 gctcaaacca cgccccctga gcatctgtct gcaaaatcaa ccgaccacga caggaaaggc
     9961 agaaacaatg cctgatattt ctgctgagcg cagaatcggc actgataatg tgctcacccg
    10021 gcgtgatccc cagccccgtt tttagaccgt tcatcctgcc actgcctcct cactgaaatg
    10081 ccctgtatga cagtgtgtgc agtactgctc cccataataa tcgtggtgac attgtctgca
    10141 gtgccagctg gctttacgca ccacgggtaa ggcatccggt acccattgct gtaagcgttt
    10201 aatcaatagt atttctctgc gctccggtct gacataaggg cactgctgac cgtgagccgt
    10261 cagcccgtcg tcagtgtgtt caaaccaggg ccgttcggtg tcgtattgcg ggtggtatct
    10321 gagcgcactg ccgcaaaggt ggcaggtgta gcgatcgtaa ggtgcagtct gtgcggtacg
    10381 ggcgcccgtc agacgtctgt tgccatcaag tgcgagaaaa gattttgcgt acatagtata
    10441 tgttccttac cgccagacga cacgcaggca acagccgtcc cggttcgggc agcgtgatca
    10501 ggtgtgaatg gcggtttcag tccggtctca gaagggaaaa tcagtgtgat ggtcgtgaat
    10561 gcgtcatcac actgaggtca gataactcgt ctgtcgggct cagctacgac tgcaaccaaa
    10621 gttgcgctca ccggtgccgt gagctttggt atgctccttc gccagatagt cagcgcgttc
    10681 cagcacctgc tgaaagccgg tgtcatcatt acgcgccagc cacaccgccg gcgtgtctgg
    10741 aaaatgcgcc agcgtggcat agcatccggc atccaccccc agggcactgc accaggcctg
    10801 tttaatcatc ccggccagtg accccggagc gcggtaatca ccggcacgac accaggtatc
    10861 ccggttgacc agcagcagca ggtgatagtg ttttttgcct ttaagctccc cgaactcccg
    10921 ggcccaggcg taatgcaggg tcgtgggatg tacgcgttta ccttcacggc gcttacgctt
    10981 ctggtaagcg tcgattcggg ctttcagggc attgatgaag cgggatatca cagctgcgtc
    11041 cgttgctgcc ggtacatccg ggagacgcag gtcaacccgc aatgccgtca ggcggggatg
    11101 aacattcagt gcgtgccgca ccgtctcacg aatacgttgc tgccagaagg ggttgtattt
    11161 gtaggtcatg gttaaatctc cgtatggttc atacggaata gccacgctgt aaaaaatgtg
    11221 cagagtccct gaggtgacct ccggcaggac actgtctcag gcactctgtg ataacccgac
    11281 tgtcgtctcc ggactgacga ttgaattccc agcgttgttt ttttaatgta aaaaaccggt
    11341 tacgggtaag gctgtgagga ggcgttatgg tgatacgcaa aacggtgtgc agggattgtg
    11401 gcttgtgctg attctggctg tcgtttcagg cgtcagtgta tttgtactgt tgcggtaggt
    11461 tacaggggag gaattatccg gaatcagcgg agcaaagcgc cccgccgcct ctgcgcttgc
    11521 tgtgccagac agtgacagta actcaccgga tccccagaaa tacgagaaaa cctcagcaga
    11581 ggtcggtaat gcccgaaaat cggcccatta ccgtccacac ctccgtacac cattcccttt
    11641 tgatttcgaa ttctttgttg tcgctatacc gcttaacgtt cccggtatta tcctggttcc
    11701 cgacaacagg aacatattca gcttttaaca acctgagaaa acccaaaaag aatcacatag
    11761 agcagataac acctggcgca ataccgctgt gattaccgaa cgttcaggtg acatatttcc
    11821 agacggaagc cggcacgcga tcgtaaagct tgttcattgt cagttctgcc agccggtgat
    11881 cgaccgcaga gttgaaggct tccagctccg cagaggtaag gttataacgt gtgtgggaaa
    11941 tcactttttc cagtgtctcc cgggatgaac aacgacggaa ctgctttggt tttaacttcc
    12001 attagcctct catcacttta tgctgcaatt aacgtgatac tgtcagcata ccgcaccaga
    12061 ctggcattct ggtaccctcc ccaaactcat tactcccatg ccagcactta accggtctct
    12121 cagattgtca ctcaccaggt cggatacgct taccgctcag acggatgctt aatgcactct
    12181 gttcacgtgg gttgcaggta cgttttatat acgcaggtta tacgccagct atcagggata
    12241 gccctgtggg tacaacgcaa tctgctgcta ctgcgtagac tggcccactg atcgcactca
    12301 cagtgtttat ttcacatata tgaactcaat tatttgatct catgtgtttg atctctctct
    12361 taatctctct cttgatctct ctcttatata tttaatacca atcagacagc cttatcccac
    12421 cagtgatacc tgtcgcctgc ccacaggtca cgggctgact caccatcacc gccttcaaaa
    12481 caactctgca attatcagaa caccagtttt ccccttgtct cacgaaagtc cctcgcttct
    12541 ttatcgctcg tacgagcggg aagcatcgtt tcgcggtagc caccggatat ctcagcatca
    12601 tgctccctgt ttatctcccg gcatgaacat tcccctactc attttttacg tttcttcccg
    12661 gttgttatgc cggtgaaggt ggtgcgttgt tttcatcacc acaccggttg cgattaacaa
    12721 catccggagg aacattttca tgaatacccc agtttcactg atggatgacc agatggtcga
    12781 catggcattt atcactcaac tgaccggcct gaccgataag tggttttaca aactcatcaa
    12841 ggatggtgac tttcctgcgc ccatcaaaat gggccgcagc tcccgctggc tgaaaagtga
    12901 agtggaagcc tggctgcagg cgcgtattgc acagtcccgt ccgtaatttt taaccactgc
    12961 cccttcaccc acagcagacg caatctccgg cctgccggcg gcgttctgct gcctgttatc
    13021 ccccgtgagg aatatgaaaa tgaaaccaca gtatcagacc cgttacgagc tgctccacga
    13081 aagctaccag aaatggctga ccggcttcac ccggcacgcc gtatcctggg gcatgtgtca
    13141 tccgaatatc cactacttcc ataatctgac gcccgggtgg gtgtcattca atggtgaaaa
    13201 accggagatt gccatcgtcc cccggagtct gcaccggctg atttatggct ctgacaaacg
    13261 ggccacgccg cccctggatg atgatctggt ggtgaactta tgtaccagcg aacatctgct
    13321 ggttcatcat ccgatgctgg aaggcattct gctgtctgaa tgtgaacgtc tgaggcagcg
    13381 ttcgctggca aataaactga tcagtctctt ccgtcagttt ggtggcacgg aactgcgcct
    13441 caaactggtc tggctctgct ggcttgattt aatgaccgga aacagccttg acgactggac
    13501 ggagaacctg aaacggaaat cagaaaaaga gctggaggaa tggatcatcg accgtcagaa
    13561 gcagaacaca gcactgacgg atctgatgga tcagtacgtg ctcctggcat accgcacaac
    13621 ggttgacgat aaccgtaact gatgccggtt gcttccggaa ggtgcgacaa aatacggaaa
    13681 tgctgctccc ggccccgcat gctcacctgc aggacgtgtt cgtcgtcatc atactgagcc
    13741 atgacggatg tcttaagggt atccagtcat tgctgagcag gcaacaccac cagaggagcc
    13801 acggacaggg atgttcttta cagcccgata cacgccaggt tctcagcaga ctgaatgtgc
    13861 aggtgcggtt attcaccaga aatggcatgc tgcggtacat acccatcctg gtgacaatat
    13921 gtacgtggcg acagcgatcc cggcactgtg gaagagactc actcagcctg caggatattc
    13981 tttccggcat ccgggaatat ccgcctgaat gcaaacagga tgatatttga atccgtaata
    14041 ctgttctttc atcataaata atttatgcag atacagttaa ccgttacagt agccggtttg
    14101 ttttaccggg aaataatatc cgtcagactg atgccggaac atatctgaaa cacgttatga
    14161 tgttttgtta tcagaaatgt tgtatattct tatccgtgac gacaatttaa tgccctctgg
    14221 catcacctga taatgcatat cctgcgatga ttcgttatat ttacgggaca cgtctctttt
    14281 cattgtgctc cttttcagac ttcattttcc ataagtattc cccggcataa aggaggtacc
    14341 tttctggcgt ttataactct ctttatcggt gatgccggta atacagggaa taccacaccg
    14401 acaaacgagg tattaacaga cgatatttca gtgtccataa tttttctttc cggttaaata
    14461 acgtcagtct gctgccctct gactgtaatc atcaaagccg gaatgcatac cgggttacaa
    14521 cgtaaaaaaa gtgatgctgg cctccggtgc agctggcggg cgaaatatgt ttacctttca
    14581 atcccccctg tctgcagtct ccactcttcc atcctcttaa gctacgtcgg ccacatgaat
    14641 gcacttccat gataacaggt attcctgtca gcataacgaa gccagttacc ttcccttcct
    14701 cctgcatttc ctgtcagaat gactatacag cgcgcagatt gcctttttat tgaccacctt
    14761 aatatatcat tataaacttc tttttatcat gttattatca ccttattatg tttgattttt
    14821 aagcagccgg agaaaaagga tggttcagaa aattttgtcg gataaggtta tgaatgaaag
    14881 aacaaatgcg tattactctt attatcttgg agagcgtaat atctccgtac tgccccttaa
    14941 tgtttatgat cccccggaac gttttatcgc ttacataaaa aaaaacagag aaaatctgaa
    15001 catcacactt tcagattttg aacttgaaca aataatctct ggtatgcgtc tgaaggccct
    15061 ggccttcctg atccccctgg aaaaaatatc ctggatcgcg ggcagtgaac gcgcatgctt
    15121 attttcctgg tatctgttga tgcagtttat ccagaataac agggcaaaaa taagcgcaga
    15181 cttgcttcaa aagaataaac tgtatcttaa agaagagtat ctggaaggga atgccttccc
    15241 gtcggactca tcgacacagt tcagacaaat actcagagtt ctcgatattc tttctgataa
    15301 gaatttgcgg gatgagtgga taatccagac caaagacaga tggatgcggg catttaaatc
    15361 aaagagtcct ttcagttacc tcctcccgga gaacgaacat gaatgcatat ggacatggaa
    15421 ttatttgaag ggaaaaaata ttgcactgga aaaactggcc agttttcctg gttctgctga
    15481 tatttaccac gcaattcacc tttcatttga catatgggtt acctgtccac tcacctcacc
    15541 tgacgatata aaaaatttca gaaacagttt caataaggcc aaggcgcagc gcaagtacaa
    15601 aaaaatgcag gaagataaag tcaatgtaca gttttttctt gacgcagaaa ccaggacgca
    15661 gctcaaagaa ttatcgaggg tcagaagact cagtaccggc gaaatgctgc atgacctgat
    15721 tgttgaagag tataaaaggt accgacattc cagataattt cagacatata ttatctccgt
    15781 gagtccccca ccacctttcc ggtgtgcggg gtttttgtct tttttcaccg ggaatacatg
    15841 tatgaatccg tctgatgcca ttgaggcaat tgaaaaaccg ctctcctccc tgccttactc
    15901 actttcccgt cacatcctgg aacatctgcg caaactcacc agtcacgaac ccgtgattgg
    15961 cattatgggt aaaagcgggg ccggtaaatc ctcactctgt aatgcactgt ttcaggggga
    16021 ggtcaccccg gtcagtgatg ttcacgccgg cacccgggaa gtgcggcgct tccgtctgag
    16081 tggccacggt cacagcatga ttatcactga cctgcccggg gtgggcgaga gccgggacag
    16141 ggatgcagag tatgaagccc tgtaccgtga cattctgcct gaacttgacc tggtactgtg
    16201 gctgattaaa gccgatgacc gtgccctgtc tgtggatgag tatttctggc gacacatcct
    16261 gcaccgggga catcagcagg tgctgtttgt ggtgacgcag gccgacaaaa cggagccctg
    16321 ccatgaatgg gatatggccg gcattcagcc ttctcccgca caggcacaga acattcgtga
    16381 gaaaacagat gcggtattcc ggctgttccg gcccgtacat ccggttgttg ccgtatcggc
    16441 ctgcaccggc tgggaactgg atacgctggt cagtgcgctc atgacagcgc ttcccgacca
    16501 tgccgccagt cccctgatga cccgcctgca ggacgagctg cgcacggaat ctgtccggtc
    16561 tcaggcccgt gaacagttta ccggtgcggt ggaccggata tttgacacgg cggagagcgt
    16621 ctgtgttgcc tctgttgccc gtacggttct gcgtgccgtc cgtgacacgg tggtctctgt
    16681 tgcccgcgct gtatggaact ggattttctt ctgaacagtc tgccagggga aatgcctgat
    16741 tacattgcgt taaccaactg aaaacatgac acaaccccct catatccacc tctttctctt
    16801 gcgtgaccgc cttgtcgtta atagaataaa acgatcgata aaacaggtat cgatcgttgc
    16861 tatcgatcgt ttatagtggt taatggcctg cgaaaaggca gctgaatctc ttcatcatgc
    16921 agaacggaat tgcacacaac ggactgatac ttctctggcg gatgagagag gggaagcatt
    16981 tatgtccggg aaaaaccact ttatctgacc atgctgtttt gtacctgccg gtatccacat
    17041 ttgtgggtac cggctttttt attcaccctc tgtaaggaaa agcttatgaa acgacatctg
    17101 aacaccagct acaggctggt atggaatcac attacgggca cccttgtggt ggcttccgaa
    17161 ctggcccgct cacggggaaa atgcgccggt gtggcggttg cactgtctct tactgctgtc
    17221 acgtcagtcc aggcgctggc tgctgacacg attgtacagg cgggtgaaac cgtgaacggc
    17281 gggacactgg caaatcacga caaccagatt gtcctcggta cggccaacgg aatgaccatc
    17341 agtaccgggc tggagtatgg gccggataac gaggcgaata ccggcggcca atggatacaa
    17401 aatggcggta ccgccaacaa cactactgtc accggtggtg gtcttcagag agtgaatgcc
    17461 ggaggaagcg tttcagacac ggttatcagt gccggaggcg gacagagcct tcaggggcag
    17521 gcagtgaata ccactctgaa cggcggtgag cagtgggtac atgaaggcgg gattgcaacg
    17581 gttaccgtca ttaatgagaa gggctggcag gccgtcaaat ccggcgcaat ggcaaccgac
    17641 acggttgtga ataccggcgc ggaaggggga ccggatgcag aaaatggtga taccgggcag
    17701 accgtctacg gagatgccgt acgcaccacc atcaataaaa atggtcgtca gattgtggct
    17761 gctgaaggaa cggcaaatac cactgtggtt tatgccggcg gcgaccagac tgtacatggt
    17821 cacgcactgg ataccacgct gaatgggggg taccagtatg tgcacaacgg cggtacggcg
    17881 tctggcactg ttgtaaacag tgacggctgg cagattatca aggaaggtgg tctggcggat
    17941 ttcaccaccg ttaaccagaa aggcaaactg caggtgaacg ccggtggtac cgccacacat
    18001 gtcaccctga aacagggtgg agcactggtc accagtactg cggcaacggt tcttggcagc
    18061 aaccgtctgg gcaatttcac ggtggaaaac ggtaaggctg acggcgttgt tctggagtcc
    18121 ggcggtcgcc tggatgtact ggaaggccat tcagcgcaga aaacacgggt ggatgacggc
    18181 ggtaccctgg cagtgtctgc cggtggtaag gcaacaggtg tcaccatgac atccggtggt
    18241 gccctgattg cagacagtgg tgccactgtt gaggggacca atgccagcgg taagttcagt
    18301 attgatggca tatccggtca ggccagcggc ctgctgctgg aaaatggcgg cagctttacg
    18361 gttaatgccg gaggactggc cagcaacacc actgtcggac atcgtggaac actgatgctg
    18421 gctgccgggg gaagtctgag tggcagaaca cagctcagta aaggcgccag catggtactg
    18481 aatggtgatg tggtcagtac cggcgatatt gttaacgcag gggagattta ctttgataat
    18541 cagacaacac cggacgccgt actgagccgt gctgttgcaa aaggcaacgc cccggtaacg
    18601 ttccataaac tgacaaccag taacctcacc ggtcagggtg gcaccatcaa tatgcgtgtt
    18661 cgccttgatg gcagcaatac ctctgaccag ctggtgatta atggtggtca ggcaaccggc
    18721 aaaacctggc ttgcgtttac aaatgtcgga aacagtaacc tcggggtggc aacctccgga
    18781 cagggtatcc gggttgtgga tgcacagaat ggtgccacca cagaagaagg tgcgtttgcc
    18841 ctgagtcgcc cgcttcaggc cggagccttt aactacaccc tgaaccgtga cagcgatgaa
    18901 gactggtacc tgcgcagtga aaatgcttat cgtgctgaag tccccctgta tgcctccatg
    18961 ctgacacagg caatggacta tgaccggatt ctggcaggct cccgcagcca tcagaccggt
    19021 gtaagcggtg aaaataacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
    19081 aacaacggcg gtattgcccg tggggccacg ccggaaagca gcggcagcta tggcttcgtc
    19141 cgtctggagg gtgacctgct cagaacagag gttgccggta tgtctctgac gacaggggtg
    19201 tatggtgctg caggccattc ttccgttgat gttaaggatg atgacggttc ccgcgccggc
    19261 acggtccggg atgatgccgg cagcctgggc ggatacctga atctgacaca cacgtcctcc
    19321 ggcctgtggg ctgacattgt ggcacaggga acccgccaca gcatgaaagc gtcatcggat
    19381 aataacgact tccgcgcccg gggctggggc tggctgggct cactggaaac cggtctgccc
    19441 ttcagtatca ctgacaacct gatgctggag ccacaactgc agtacacctg gcaggggctc
    19501 tccctggatg acggccagga taacgccggt tatgtgaagt tcgggcatgg cagtgcacaa
    19561 catgtgcgtg ccggcttccg tctgggcagc cacaacgata tgacctttgg cgaaggcacc
    19621 tcatcccgtg acactctgcg cgacagtgca aaacaccgtg tgagtgaact gccggtgaac
    19681 tggtgggtac agccttctgt tatccgcact ttcagctctc ggggtgacat gagcatgggt
    19741 acagccgcag ccggcagtaa catgacgttc tcaccctcac agaatggcac atcactggac
    19801 ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca ccctgggcgt tcaggccggt
    19861 tatgcccaca gcgtcagcgg cagcagtgct gaaggttata acggtcaggc cacactgaat
    19921 gtgactttct gataatcagg tcactcccct tcaggccccg gtcatcatga ccggggcctt
    19981 ttacttacag ggagcaacag atatgccggg ttgtacatca cgtttattgc ccgaaggccc
    20041 ttttactgcc aaatggcatc tgaattgatc acagtctggc cagttattac ccgatcccga
    20101 tggggatatt gggtgatctc cttccatgaa aatacgcagc tcaccagtgg agggcttctc
    20161 agacaataaa cgtcattcat ttcatctgtc aggccgcgtc ttctccggga gacgcggctt
    20221 tttcatttat accgctaact cattcataag gagcaaataa tgcgattagc aagtcgtttt
    20281 ggtcatgtaa accagatacg ccgtgaccgc ccgctgacac atgaagaact gatgcactat
    20341 gtgcccagta tttttgggga agaccggcat acctcccgca gtgaacgcta tgcgtacatt
    20401 cccaccatca ccgtactaga aagcctgcag cgggagggct ttcagccctt cttcgcctgc
    20461 cagacccgcg tgcgcgaccc gggccgccgg ggatacacca aacacatgct gcgtctgcgc
    20521 cgtgacgggg agataaacgg acaacatgtc cctgaaatca tcctgctgaa ctcccatgac
    20581 ggtacttcca gctaccagat gctgccgggt tacttcaggt tcgtctgcca gaacgggtgc
    20641 gtctgtggcc agtctctggg ggaagtgcgt gttccacacc ggggaaatgt ggtggagaaa
    20701 gttatcgaag gggcttacga ggtggtgggc gtgtttgacc ggatagagga gaagcgtgat
    20761 gccatgcagt cgctggtcct gccgccaccg tcacgccagg cgctggcaca ggcggcactg
    20821 acttaccgtt atggtgacga acatcagccc gtcaccaccg ccgacattct gacgccacga
    20881 cgccgggagg attacggtaa ggacctgtgg agtgcttatc agaccatcca ggagaatatg
    20941 ctgaaaggcg ggatttccgg tcgcagtgcc agaggaaaac gtatccatac ccgtaccatt
    21001 cacagcatcg acaccgacat taagctcaac cgggcgttgt gggtgatggc agaaacactg
    21061 ctggagagca tgcgctgatg ccttttccct gaaagcgcag tcctgactca cttccgtccc
    21121 tgcctccgga cattcttcca ttcatttact ttttataagg aataatctca tgacaaccgt
    21181 tacgcataat tccaccacac cttctgtttc cgtaaccgct gcatcaggga ataacccacc
    21241 tcagctggtt gctactctcg tccctgatga acagcgcatc agcttctggc cgcagcattt
    21301 tggcctcatt ccacagtggg tgaccctgga gccccgtgtc ttcggctgga tggaccgtct
    21361 gtgcgaagac tactgcggtg gtatctggaa tctgtacacc ctgaacaacg gcggggcatt
    21421 tatggcaccc gaaccggatg acgatgatga cgaaacatgg gtactgttca atgccatgaa
    21481 cggtaaccgc gctgaaatga gcccggaagc cgccggtatt gccgcctgtc tgatgacgta
    21541 cagtcatcat gcctgtcgta tggagaatta tgccatgacg gtccattatt accggttgcg
    21601 ggattacgcc ctgcagcatc cggaatgcag cgccattatg cgcatcatcg actgaatgga
    21661 gagaagcaca atgcaacaac tttcctttct gcccggagag atgacgcccg gcgagcgcag
    21721 cctcattcaa cgggccctga aaaccctgga ccgccatctt catgaacccg gcgtggcctt
    21781 cacctccacc cgtgcagcac gggaatggct gattctgaac atggcgggac tggagcgtga
    21841 agaattccgg gtgctgtatc tgaacaacca gaatcagctg attgccggtg aaaccctctt
    21901 caccggcacc atcaaccgca cggaagtcca tccccgggaa gtgattaaac gcgccctgta
    21961 ccacaatgcc gctgccgtgg tactggcaca caatcacccg tccggtgaag tcacacccag
    22021 taaggcagac cggcttatca ccgaacgtct ggtacaggca ctgggcctgg tggatatccg
    22081 ggtgccggac catctgatag tcggtggcaa ccaggttttc tcctttgccg aacatggtct
    22141 gctttaaccc gtcacaacca catcacacct gttttcactt ttatcttctg tcttcagagg
    22201 tatcccatta tgaaaattat cacccgtggt gaagccatgc gtattcacca acaacatccg
    22261 acatcccgtc tttttccgtt ctgtaccggt aaataccgct ggcacggcag tgctgaagcg
    22321 tatactggtc gtgaagtgca ggatattccc ggtgtgctgg ccgtgtttgc tgaacgccgt
    22381 aaggacagtt ttggtccgta tgtccggctg atgagcgtca ccctgaactg agtgggaatt
    22441 ctgatgagca gaattatcgc cactaccgga ccattcttag ccgattttct gtaaggattt
    22501 tatcgtgtca gacacactcc ccgggacaac acttcccgac gacaatcacg accgcccctg
    22561 gtgggggctg ccctgcaccg tgacgccctg tttcggggca cgtctggtgc aggagggtaa
    22621 ccggttgcat taccttgccg
//
LOCUS       DAPGWD010000062        22397 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPGWD010000062 DAPGWD010000000
VERSION     DAPGWD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.062"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            116..547
                     /gene="osmC"
                     /locus_tag="RHX88_002374"
     CDS             116..547
                     /gene="osmC"
                     /locus_tag="RHX88_002374"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDV2231051.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(582..1352)
                     /locus_tag="RHX88_002375"
     CDS             complement(582..1352)
                     /locus_tag="RHX88_002375"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDV2231052.1"
                     /translation="MNLQGKTALVTGSTSGIGLGIAQTLANAGAQVILNGFGDVDAAI
                     NAVSRMGKTPGYHEADLSDESQIINMMAYARREFGGVDILVNNAGIQYVSPVESFPVE
                     KWNAIIAINLSSVFHTTRLALPEMRTRNWGRIINIASVHGLVASKDKSAYVAAKHGVV
                     GLTKTIALETAQTGITCNALCPGWVLTPLVQQQIDKRIAQGEDAESARDALLAEKQPS
                     REFVTPQQLGELALFLCSDAAAQVRGVAWNMDGGWVAQ"
     gene            complement(1364..2755)
                     /locus_tag="RHX88_002376"
     CDS             complement(1364..2755)
                     /locus_tag="RHX88_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDV2231053.1"
                     /translation="MSVLIALAALGLLMLAAYRGYSVILFAPIAALGAVLVTDPGAVG
                     PAFTGLFMEKMVGFVKLYFPVFLLGAVFGKLIELSGFSRSIVAAAINILGRRHAIPVI
                     VLVCALLTYGGVSLFVVAFAVYPFAAELFRQSNIPKRLIPATVALGAFSFTMDALPGT
                     PQIQNIIPTSFFGTNAWAAPWLGLIGSLFIIAVGLIYLERQRRKAQAKNEGYGTELLN
                     EPETPDNINLPNPLIAISPLILVGLLNLLFTWWIPVWYGASHQLELPGLAAPITTDVA
                     KITAIWAVEAALIAGILLVVIFGYRNIHGRLAEGSRTAVGGAILAAMNTASEYGFGAV
                     IAALPGFLVLSKALAAIPDPLLNEAISITTLAGITGSASGGMSIALAAMSDTFIAAAH
                     AANIPLEVFHRVASMASGGMDTLPHNGAVITLLAITGLSHRQAYGGIFAITIIKSLAV
                     LFIIGVYYTTGIV"
     gene            complement(2801..3724)
                     /locus_tag="RHX88_002377"
     CDS             complement(2801..3724)
                     /locus_tag="RHX88_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HDV2231054.1"
                     /translation="MQTEQFAVLGAGLMGVGIATHFARFGHDIWLYDTEQSRIAEIHV
                     VAGGILDELISCEQFTAPEKENVLKRLHGTTSLADIADSQLLIEAIPERLELKHALYT
                     ELETLISPEAIVASNTSGLPPDDLAAKMRYPQRLLIAHFWHPPHFIPLVEIIPGSVTE
                     PKHLHWLQHLLVSLQLEAVLLDRAAPGFVGNRLQFALLREALHIVKSGIASAEVVDQV
                     MRASLGRRYAMVGPLEAADMTGLSTVQDICRHLLPELATGSDMMSLVAEKVERGDIGV
                     RSGQGFYCWDESRKQYIQQRREHQLRFALKP"
     gene            complement(3714..4919)
                     /locus_tag="RHX88_002378"
     CDS             complement(3714..4919)
                     /locus_tag="RHX88_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDV2231055.1"
                     /translation="MQDVVIVAATRTPIGCFHGALAPLSAVDLGAATIRSLLEKTALA
                     AEQIDEVIFGQVLTAGSGQNPARQSALKAGLSHNTPAVTINLVCGSGLKAVHQAVQAI
                     RCGDAQIIIAGGQESMSNAPYFIDGARAGLRLGHTSMKDSVVHDGLWDAFNDYHMGIT
                     AENLAQKYAISRERQDTFALRSQQKAAAAMEAGRFADEITPVSVPQGKKPPRWVNTDE
                     QPRSGTQLEKLAQLKPAFLPGAGSVTAGNASSINDGAAAVMLMRASKAAELGLPVLAR
                     VAGYAVSGVDPAFMGIGPVAATRECLKRCGWTLEDIDLIEANEAFAAQALAVGDELNW
                     DCDKVNVNGGAIALGHPIGASGCRILVTLIYEMARRNAQKGLATLCVGGGQGVALTLE
                     RTTGEHHAN"
     gene            complement(4929..5585)
                     /locus_tag="RHX88_002379"
     CDS             complement(4929..5585)
                     /locus_tag="RHX88_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HDV2231056.1"
                     /translation="MLTREQMAMRVARELRDGYYVNLGIGIPTLVANYIPDGMDVMLQ
                     SENGLLGMGAFPTEDELDADMINAGKQTVTARKGAAIFDSAQSFAMIRGGHVDLTVLG
                     AFEVDVNGNIASWMIPGKMVKGMGGAMDLVAGAENIIVVMTHASKSGESKLLPDCTLP
                     LTGAGCIKRVLTDLALLEIADGAFILRERAPGISVAEIIAKTAGKLFVPEHVPEMQFN
                     "
     gene            complement(5588..6295)
                     /locus_tag="RHX88_002380"
     CDS             complement(5588..6295)
                     /locus_tag="RHX88_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDV2231057.1"
                     /translation="MAGLDKRVATYHEALEGLTNEMTILAGGFGLCGIPENLIAEVRH
                     RGVTGLTVVSNNCGVDGFGLGVLLESRQIRKVVASYVGENALFEKQVLNGELEAILTP
                     QGTLAEQLRAGGAGIPAFFTATGYGTPVAEGKEVREFNGRPYIMEQAITGDFALVKGW
                     KADWYGNVIYRHTAQNFNPLMAAAGKITVVEVEEIVPPGELDPAAIHTPGIFVNRVIQ
                     GQFEKRIEQRTLSRKGA"
     gene            6437..7327
                     /locus_tag="RHX88_002381"
     CDS             6437..7327
                     /locus_tag="RHX88_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2231058.1"
                     /translation="MKMSIKQLRAFLAVAHTLNFAHASERLNMSQPALSLAIKGLEDA
                     LGGALLLRTTRKVTLTQEGETLLSMARPLLADWDNTEEAMRQRFTLRRGKVSVAAMPS
                     FAANVLPPVLKTFRDCYSGINVTVHDVVNEQVIEMVSEGRVEMGIAFEPDYCGHVHFT
                     PLGVDRFVAIVPADSRLANRQRVSWRDLLTLDFITLQRPSAVRLMLEEQLAQSGTQLE
                     VALESHQLVTVGRLVANGLGGSAVPALCRRQMSELGAVCLELDNPVIERRVGVLRSSH
                     HKLSTAAQALMETLKKTYDA"
     gene            complement(7364..8290)
                     /locus_tag="RHX88_002382"
     CDS             complement(7364..8290)
                     /locus_tag="RHX88_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2231059.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQDNHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(8283..9269)
                     /locus_tag="RHX88_002383"
     CDS             complement(8283..9269)
                     /locus_tag="RHX88_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2231060.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQVLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(9266..10162)
                     /locus_tag="RHX88_002384"
     CDS             complement(9266..10162)
                     /locus_tag="RHX88_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDV2231061.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(10159..11181)
                     /locus_tag="RHX88_002385"
     CDS             complement(10159..11181)
                     /locus_tag="RHX88_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2231062.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(11183..12733)
                     /locus_tag="RHX88_002386"
     CDS             complement(11183..12733)
                     /locus_tag="RHX88_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2231063.1"
                     /translation="MKRSISFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQHKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(12747..13328)
                     /gene="ddpX"
                     /locus_tag="RHX88_002387"
     CDS             complement(12747..13328)
                     /gene="ddpX"
                     /locus_tag="RHX88_002387"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDV2231064.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            complement(13586..15985)
                     /gene="dosP"
                     /locus_tag="RHX88_002388"
                     /gene_synonym="pdeO"
     CDS             complement(13586..15985)
                     /gene="dosP"
                     /locus_tag="RHX88_002388"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDV2231065.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(16010..17392)
                     /gene="dosC"
                     /locus_tag="RHX88_002389"
     CDS             complement(16010..17392)
                     /gene="dosC"
                     /locus_tag="RHX88_002389"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDV2231066.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            complement(17764..19083)
                     /locus_tag="RHX88_002390"
     CDS             complement(17764..19083)
                     /locus_tag="RHX88_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDV2231067.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(19214..20749)
                     /gene="gadC"
                     /locus_tag="RHX88_002391"
     CDS             complement(19214..20749)
                     /gene="gadC"
                     /locus_tag="RHX88_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDV2231068.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(20905..22305)
                     /locus_tag="RHX88_002392"
     CDS             complement(20905..22305)
                     /locus_tag="RHX88_002392"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV2231069.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
BASE COUNT         5537 a         5818 c         5690 g         5352 t
ORIGIN      
        1 aatttaagtg cagaagtaat attttcgccg tattttattc ggaatatcct gcttatcctc
       61 gtgcggtttc tcacgtagtc tataatttcc tttttaagcc cacaggagag caacaatgac
      121 aatccataag aaaggtcagg cacactggga aggcgatatc aaacgcggga agggaacagt
      181 atccaccgag agcggcgtgc tgaaccaaca gccgtatgga tttaacacgc gttttgaagg
      241 cgaaaaagga accaaccctg aagaactgat tggcgcagcg catgccgcat gtttctcaat
      301 ggcgctttca ttaatgctgg gggaagcggg attcacgcca acatcgattg ataccaccgc
      361 cgatgtgtcg ctggataaag tggatgccgg ttttgcgatt acgaaaatcg cactgaagag
      421 tgaagttgcg gtgccgggta ttgatgcctc tacctttgac ggcatcatcc agaaagcaaa
      481 agcaggatgc ccggtctctc aggtactgaa agcggaaatt acgctggatt accagttgaa
      541 atcgtaaagc attgccggat gacgcgtcat ccggcgaaca cttactgcgc tacccaaccg
      601 ccatccatat tccacgccac cccacgcact tgtgctgccg catccgaaca gagaaacagc
      661 gccagttcac ccagttgttg cggagtcaca aattcacgag aaggctgttt ttctgccagt
      721 aaggcgtcac gcgcgctttc cgcatcttcg ccttgcgcaa tgcgcttatc aatctgctgt
      781 tgtaccagcg gcgtcagcac ccagccaggg cagagcgcgt tgcaggtaat acccgtctgc
      841 gcagtttcca gagcaatggt ctttgtgagt cccaccacgc catgttttgc cgcaacataa
      901 gctgatttgt ctttcgacgc taccagccca tgcacagagg caatgttaat gatccgaccc
      961 cagttacgag tgcgcatttc tggcagtgcc agacgcgtgg tgtgaaagac agaagagaga
     1021 ttgatagcga taatggcgtt ccatttttct acagggaaag attcaaccgg ggagacatac
     1081 tggatcccgg cgttattcac cagaatgtcc acaccgccaa attcccgccg tgcgtaggcc
     1141 atcatgttga taatttgcga ttcatcgctg aggtctgcct catgatagcc aggcgtttta
     1201 cccatacgag aaaccgcatt gattgccgcg tcaacatcac cgaagccgtt gaggatcact
     1261 tgcgcgccag cgtttgccag tgtctgagca ataccgagtc cgataccgct ggtggacccc
     1321 gtcaccagcg cagttttacc ttgtaaattc atgatgattt tccttagaca atgccggtgg
     1381 tgtagtacac gccgatgata aacagcacgg ccagactttt aatgatcgta attgcgaaga
     1441 tgccgccgta agcctgacgg tgactcagcc cagtaattgc cagcagggtg atcacagcgc
     1501 cgttatgggg caatgtatcc atgccgccac tggccataga tgccacgcgg tgaaaaactt
     1561 ccagtggaat attggcagcg tgcgcagcgg caataaaagt gtcggacata gccgcaagag
     1621 caatactcat cccgccggac gctgaaccgg taattccggc aagtgtggtg atgctgatgg
     1681 cttcgttgag taatggatca ggaatggcgg ccagcgcctt tgagagcacc agaaaaccgg
     1741 gtagggcggc gataaccgca ccgaagccat attctgaggc ggtgttcatc gcagccagaa
     1801 ttgcgccgcc taccgctgta cggcttcctt ctgccagtct tccatgaata ttgcgatatc
     1861 caaaaatcac taccagcaaa attccggcaa tgagcgcagc ttcgacggcc cagattgcgg
     1921 tgattttggc gacatctgtg gtaatcgggg cggcaagtcc cggcaactcc agttggtggc
     1981 tcgcaccgta ccaaacaggg atccaccagg taaacaacaa gttcaacagc cccaccagaa
     2041 tcaatggtga aatcgcaatg agtgggttcg gtaaattgat gttatctggt gtttcaggtt
     2101 cattaagtaa ctctgtgccg taaccttcat tttttgcctg cgctttacga cgctgccgct
     2161 ccagatagat aagcccgacg gcaatgataa acagtgaacc aataagcccc agccacggcg
     2221 cggcccaggc gttggtgcca aagaaactgg taggaataat gttctgaatt tgtggcgtgc
     2281 cgggcagagc gtccatagta aaagagaatg cccccagggc caccgttgcc ggtataagtc
     2341 gttttgggat gttactctgg cggaacagtt cagccgcgaa cggataaacg gcaaatgcca
     2401 ccacaaacag cgacactccg ccataggtga gtaatgcaca taccagcaca atcaccggaa
     2461 tcgcatgccg ccggccgaga atgttgattg cagcagcgac aatcgaacga gaaaatcctg
     2521 aaagttcaat cagttttccg aacacggccc cgagcagaaa caccgggaag tacaatttca
     2581 cgaagccgac cattttttcc atgaacaggc cggtaaatgc tgggccaacg gcccccggat
     2641 cggtgacaag aacagcgcct aatgctgcga tgggggcaaa aaggataacg ctgtatccgc
     2701 gataggctgc cagcattagc agcccaagcg cggcaagggc gattaataca ctcatcggga
     2761 ctcctcaata tcgttgttat agtgggtaca gcgagagaag ttatggttta agggcgaagc
     2821 gaagttgatg ttcccgacgc tgctggatgt actgcttccg cgattcatcc cagcagtaaa
     2881 atccctgacc gctacgtacg ccgatgtctc cacgttccac tttttcggca actaatgaca
     2941 tcatgtcgct gccagtcgct agttcaggga gcagatggcg acaaatatcc tgcaccgtcg
     3001 aaagtccggt catgtcggcg gcctccagcg ggccgaccat ggcataacgt cgcccaagcg
     3061 aggcgcgcat cacctgatcc accacttctg cactggcgat gccgcttttt acgatatgca
     3121 gggcttcacg caacagtgca aattgcagac ggttgccgac aaatcctggc gcagcgcgat
     3181 ccagcaacac tgcttccagt tgcagtgaca caagcaagtg ttgcagccag tgcaaatgct
     3241 ttggctcggt aacgctgccg gggataatct ccacaagtgg aatgaagtgc ggcggatgcc
     3301 agaaatgggc gatcagcagt cgttgcgggt agcgcatctt cgcggccaga tcatcgggcg
     3361 gcagaccgct ggtattgctg gcgacaatgg cctcaggaga aataagcgtt tcaagctcgg
     3421 tatataatgc gtgttttagt tcaagtcgct ccgggatcgc ctcaatcagt agttgcgagt
     3481 cggcgatgtc tgcaagagaa gtcgtgccgt gcagacgttt aagcacattc tctttttccg
     3541 gggcagtaaa ctgttcgcag ctaatcagct catcaagaat tccgcctgca actacgtgga
     3601 tttctgcaat ccggctctgc tcggtatcgt acagccagat atcatgacca aagcgggcga
     3661 aatgagtggc aatccccacg cccatcaatc cggcgcccag taccgcaaat tgttcagttt
     3721 gcatggtgtt ctcctgtggt tctttccagt gtcagcgcca cgccctgacc gccaccgacg
     3781 cacagcgtcg ccagcccttt ttgcgcatta cgtcgcgcca tttcataaat gagagtgacc
     3841 agaatgcggc aaccggaagc gccaatagga tgcccaagtg cgattgcgcc gccgttaaca
     3901 ttcactttgt cgcaatccca gttcagttcg tcgccaacgg ccagcgcctg cgctgcgaaa
     3961 gcctcgttgg cttcaataag atcaatatct tccagcgtcc atccgcaacg cttaagacat
     4021 tcgcgggtag cggcgaccgg accaatgccc ataaaggcag gatcgacgcc agaaacggca
     4081 taacctgcca cccgagccag cactgggagg cccagttcag cagctttgct ggcgcgcatt
     4141 aacatgactg cggcggcacc atcattaatg gaagaggcat ttcctgcggt gacgcttccc
     4201 gcccccggaa gaaaggccgg tttcagttgc gctagttttt ccagttgcgt cccggaacgt
     4261 ggttgttcgt cggtatttac ccagcgtggt ggttttttac cttgtggtac gctcaccgga
     4321 gtaatttcgt ctgcaaagcg cccggcttcc attgcggcag cggctttttg ctgagaacgt
     4381 agcgcgaatg tgtcctgtcg ctcgcgactg atggcatatt tttgcgccag gttttctgca
     4441 gtaatgccca tgtggtaatc attgaaagca tcccatagtc cgtcatgaac cacgctgtct
     4501 ttcatgctgg tatgcccgag gcgtaaacct gcccgcgcgc catcaataaa atagggcgcg
     4561 ttgctcatgc tttcctggcc acctgcgata attatctgtg catcaccgca acggatcgcc
     4621 tgcactgcct ggtgtacggc tttcagaccg gaaccacata ccaggttgat ggtaacggcg
     4681 ggtgtattgt ggcttaaccc ggctttgagc gccgattgtc gtgccgggtt ttgtccgctt
     4741 ccggcggtca ggacctgacc aaaaatcact tcgtcgatct gttctgctgc aagtgcggtt
     4801 ttctccagca acgaacggat ggtggcagca ccgagatcaa ccgcagaaag tggggcgaga
     4861 gcgccatgaa aacagccaat cggtgtgcgc gtagcggcaa caataacaac gtcctgcatg
     4921 gtgattcctc agttgaattg catttccggg acatgttccg gcacaaacaa cttaccggcg
     4981 gttttggcga tgatttccgc aacgctgatt cccggcgcgc gctcgcgcag aatgaaagct
     5041 ccgtcagcaa tttccagcag agcaaggtcg gttaagacgc gtttgatgca tccggcgccg
     5101 gtcaacggaa gggtacagtc aggaagcagt ttggattcgc cggatttgga ggcgtgggtc
     5161 attaccacaa taatattctc ggcaccggca accagatcca tggcaccgcc catgccttta
     5221 accatcttgc cggggatcat ccatgaggca atgttgccat tcacatccac ttcaaatgcg
     5281 ccaagtacag tcagatccac atgaccgcca cggatcatgg cgaatgattg cgccgaatcg
     5341 aaaattgccg cgcctttccg cgcggtaacg gtttgtttac cggcattaat catgtcggcg
     5401 tccagttcgt cttccgtagg aaatgcgccc attcccagca aaccgttttc tgactgcaac
     5461 atcacatcca tcccgtcagg aatgtagttt gccaccaggg tcgggatacc aatgccgaga
     5521 ttgacgtaat agccatcgcg caattcgcgg gcgactcgca tcgccatttg ttcacgggtt
     5581 aacatggtca ggctcctttg cggctaaggg tgcgttgttc aatgcgtttt tcaaattgcc
     5641 cctgaatgac gcgattaaca aaaatccccg gggtatggat agcggcagga tcaagttcac
     5701 caggtggcac tatttcttcc acttccacca cggtgatttt tccggcagcc gccatcagtg
     5761 ggttgaagtt ttgcgcggta tgacgataaa tcacgttgcc gtaccagtcg gctttccagc
     5821 ctttgaccag ggcaaaatca ccggtgatag cctgttccat gatgtacggg cgaccgttga
     5881 attcgcggac ttctttccct tcggcaacgg gagtaccgta accggtggca gtaaagaacg
     5941 ccgggatacc cgcgccgcct gcgcgcagtt gttctgccag ggttccttgt ggtgtgagaa
     6001 tcgcctccag ttcgccgtta agcacctgtt tttcaaacaa agcattctcg ccaacataag
     6061 aagcgacgac tttacggatc tgtcggcttt ccagtagtac acccagtcca aaaccatcta
     6121 cgccacagtt attggaaacc accgttaatc cggttactcc acggtggcga acttcagcaa
     6181 tcagattctc cgggatgccg cacaggccaa aacctcctgc cagaatcgtc atctcattgg
     6241 taagtccttc aagcgcctcg tgatacgtag cgacccgttt atccaaccca gccatacagt
     6301 tgccctccag ttgtcctttt caacatcatt aagggcaaaa aatgatttgt taattttaaa
     6361 tttgtgactc attcatttgt attacttatt aatataatgt taattaaatg tattcagtag
     6421 gttaacggaa tgccagatga aaatgagcat caaacagtta cgcgcgtttt tagcggtagc
     6481 tcacactctg aactttgccc atgccagtga acggctgaat atgtcgcagc ctgcgctaag
     6541 tcttgccatt aaaggactgg aagatgcctt agggggcgct ttactgctgc gtactacgcg
     6601 aaaagtgaca ttaacccagg agggggaaac gctattgtcg atggcacggc ctttgctggc
     6661 tgactgggat aacactgagg aggccatgcg tcagcgattc acgctcaggc ggggcaaagt
     6721 ctcggtggca gcgatgccgt catttgcagc caacgttctg cctccggtgc tgaaaacctt
     6781 ccgcgattgt tattcaggga ttaatgtcac cgtgcacgat gtggtgaatg agcaggtgat
     6841 cgaaatggtg agcgaagggc gtgtggagat gggtattgcc tttgaacctg actattgcgg
     6901 ccatgtgcat tttaccccac tgggcgtgga tcggtttgtc gctattgttc cagccgattc
     6961 acgactggca aatcggcaac gcgtgagttg gcgggatttg ctgacgctcg attttatcac
     7021 cctccagcga ccgtcagccg tcaggttgat gctggaggag cagttggcac aaagtggaac
     7081 gcaactggaa gtggcgctgg aaagccatca actggttacg gttgggcggc tggtggcaaa
     7141 cggtttggga ggaagcgctg tgcctgcgct gtgccgcagg cagatgagcg aactgggcgc
     7201 agtgtgtctg gaactggata atccagtgat tgagcgacga gtaggcgtac tgcgttcgtc
     7261 acaccataag ctttctactg cggcgcaggc gttgatggaa acgttaaaaa aaacctatga
     7321 cgcataacgt ttccgcagac atgcaatagc cggatgccgc gtcctacagc gcccgccagc
     7381 aacggatctc acgtccgtca gcccgcgtcg ttaatgattg ccggacttcg catccgctgg
     7441 ttgcaagggg gcaacgttcg tagaaaaaac agccttgcgg caacgtgcgg tttcccggca
     7501 gatccgtttt acgtaatgcc cattcttcct ccagcggttt atcaatggcg gggagggaat
     7561 ccagcaataa tcgggtatat ggatgtgccg gagcagtcaa cacctgttgc gcttccccta
     7621 gttccacaat ctgcccgaga tacatcaccg ccacccgatc gctcatatga cgtatcaccg
     7681 agacattgtg tgaaatcagc acataagtca gcccgtgatt gtcctgtagc gttaccagta
     7741 aattgaggat ctgcgcctgc acggagatat ccagcgcaga ggttggctca tcaagcacaa
     7801 tcacgtcagg ctgcgaagag agtgctctgg caatggcgat acgttgccgc tgcccgccgg
     7861 agaacgcatg aggcaggcgg tcgagatact ccggacgaat acccacctgc acagccagct
     7921 cctctgccaa cgctcgccgc tgttgttcac tactacgctt agctatccag agcggttctg
     7981 tgatgatgcg ccacaccggt aagcgcggat tgagcgaaga gagcgggtcc tgaaacacca
     8041 tctgcataat gcgttgtgag ccagaacgga tgtactgccc gtggctcggt tgcagcatgc
     8101 ccattaaaag ctgtgcgagg gtgcttttgc cgcagcctga ctcgccgacg atccctaagg
     8161 tttcaccacg gcggatctgt aaatcaatac cattaatggc atgaacatgt tccgtagttt
     8221 taccaagcca gtttttacgg gccgggaaat tgatatggac gtcgcgtaac gttaataacg
     8281 tgtcagacac ttatgacctc ctgctgcgga taccaacagg cgcagcgctg gttgttgtca
     8341 ccacacgctg tcagcgccgg gacgttttca cactgtgcac ctgccgcata acaacgatcg
     8401 cgaaaagcgc agccttcagg caaatgggtg aggtttggta ccgtccctgg aatggcgggt
     8461 aatagctggc gtggtactcc atgttctggt gcgcattgga gcaaaccaat ggtatacgga
     8521 tgccggggat gatggataac atctgccgtc acgccgcttt caattacgct tccggcatac
     8581 atcacgtaaa cgctatcgca cagttgcgac accacgacca tatcatggct gatgaacagt
     8641 accgcagttc cactggcgcg ggctttatgt ttaagcaaac gcagtacctg caactgtagc
     8701 gtgacgtcca gcgccgtagt cggttcgtcg gcaataatca attgcggctc gcaggagaat
     8761 gccagcgcaa tcattacccg ctggcgcata ccacctgaaa gctcaaacgg atagcgcgac
     8821 ataacttcca cggcatccgg gatttgcatc tcttccagca gggcaatcgc tttagctctg
     8881 gcttcccgac gacttattgg ttgatgatgg cggatcacgt ccatcatctg aagacctatt
     8941 cgacgtgtcg gattgagggc ggtcatcggt tcctgaaaga tcatcgccac tcgtgcgccg
     9001 cgccactgac gaagctgttt ttcccgagcg ttaagaacat cgtctcccag cagtgaaatc
     9061 tgcccccgat gtacgcaata actgcccgtc ggtagcagac gcataatcag cattgcggtg
     9121 actgatttac ctgagccgga ttctcccacc agaccgacaa tttcaccgcg gttaatctgc
     9181 aaggacacat tgttgagcgc gtgaacatca ccgttaaaac cggggaaact caaatgcagc
     9241 tgttgaatgt ccagaacggg ttgggtcatg actgctttcc tcctgctttc gggtcaagca
     9301 gatcgcgaat accatcacca aagagattaa acccaacggc ggtaagcaaa atcgctgccc
     9361 ccggaaatgc gcaataccac cactgatcga gtacatagtt gcgaccattc gctaccatcg
     9421 cgccccattc agcactcggt tgttgagcac ccaggccaat aaatcccaac gtggcggcca
     9481 ttaaaatcgc gctaccgata tccagcgatg cctgcacgat cagcggcggc agggagttac
     9541 gtaaaatatg ccagctgatc agatgccaac gcgacgcacc aaaggttttc gccgcctgaa
     9601 cataggtata ctggcgtaca actaatgctt gcccccgcgc caggcgcaca taaaagggaa
     9661 ttcgcacaat agcaatcgcc agcatggcgt taaataaact cggcccgaga gcggcagcca
     9721 gtgccattgt cagtaccaac gaaggaatcg acagcataat gtccatgatg cgcatgatga
     9781 tggcgtctgc gcgtccgcca agcacaccgg atagacatcc gagtagcgaa ccaatcatac
     9841 ccgcaatggc aaccacgact aatccggcga gaattgattg ctgactgccg accagtacgc
     9901 ggctgaacag atcgcgtccc acttcatcgg tgccaaacca gtgcgccgca gaaggcggca
     9961 aaaggcgggc ggttaaatca atggcattgg gatcatgcgt cgccagccac ggtgaaaaaa
    10021 tcatcatcaa tagcattaat acaatgatga ctgcgccggt cacggtgagc ggactgcctt
    10081 tcagcatcca taccagtttt gcaccgttaa atcgcgtttg tttttgtggg cgtacggcgg
    10141 acgtttcctc gcttagcatc attcaccacc tccacgtccg atacgcggat caatccacaa
    10201 atagagcaaa tccaccacca ggttgaccag cacataagca aatgaaacca cgacggcaaa
    10261 gcccatcact gccgggaagt cgagcgcctg tattgatgtt actacccatg cacccattcc
    10321 gggccaggca aaaacggttt cggtgagcac tgcgccatac aacaaatcgc ccagcgccaa
    10381 acccaatacg gtaatcgatg ggatcaacgc attgggtagc gcataacata aaacgatata
    10441 ccagccgggc aagccgctgg cccgggcggt acgaatgtag tcttcactca attgttccag
    10501 catcgctgag cggatctggc gagcgacaat tcccaggtga acgaacgcca gcgttaatgc
    10561 cggtaagatg agatgttgca acgcattgaa gaagacttca ccgttgcctt caagcaacgc
    10621 atcgagcaga taaaagccgg taacgtgcgt tggtggatcc agccagtcat caagccttcc
    10681 gccgccggga agtatttgta gatgaccata aaacagcaca atgacgccca gcccaagcca
    10741 gaacgcaggt gtggagattc cggtaatggc cattattcgc accagatgat ccagccagcg
    10801 atttcgccag actgcagaga gtatgcccag cgggatgcca atcaagagtg ccagcagcag
    10861 ggcacaaaaa gccagctcca gcgtcgccgg gaaaaatata cgcaactctt ccagcaccgg
    10921 acgcccggta cgaatggatg ttcccaggtc accatgaaac aggtcgctga cgtagcggta
    10981 aaactgtaca tacagtggct ggtccagtcc cagttgctgg cgaatatttt ccacgatagc
    11041 atcgctggcg cggtcacccg ccagtaaccg cgccggatcg ccagggatca gatgcgagat
    11101 aataaacgta atcacgcaga cgcccgccac cacgagcacc agcccccagc agcgttggcg
    11161 taaaatactc cagaaggtca cgttatttac tcatggtatt gatattgaag acctgttcca
    11221 gcatgggatt gaacacaaag cctttcacct ctttgttcat cgccagttgg tagtttttct
    11281 ggaacaggta cacataagca gcgtcatcaa tgacgatttt ctgtgcctgc tggtagtccc
    11341 gggtacgctg cgtctggtcg gtggtcgcaa gcgcattgcg cagtaactta tcgacctcac
    11401 tgttttcata gaacgagcgg ttacccggca gacctttttt gtctgactca aaccagtaat
    11461 tcataaacat atacgggtcg gcaaaatccg gactccagtt gccaatcgca atgtcgtaat
    11521 cacctttacc cactctgtcg cgcatggtgg cgttcgccag cttttccagc ttcacattga
    11581 tgcccagctt gttgagactg gattgtgtcg ccaaagcaat aggctcccag ttcggatcgt
    11641 tatcggagta gagaaacgtc aggctggtgg gtttgctcgt cactttatcc cattcggctt
    11701 tggctttcgt ttcgtcatgg ttgtattgca ttgccgtcgc atcgtagccc cacatgcctt
    11761 ccggaatcgg gccgcgcatc tgttttccgt taccactcag aatgccgtta accatgccct
    11821 gataatcggt agaccaggaa atggcccgac gcagatccgc ctgattaaga ggcgctttgc
    11881 tgttattcag atacagatag gtaacgcgca gtgacggata ctctgccaca ttgactttat
    11941 tttcctgctt cagggcgttg agttgatcca ccggcagcgc atcggcaatg tcaatgtcgc
    12001 cacgggagag ctgcaggcga cgggaggcac tttcaccgat aatttttacc gatacccgtt
    12061 tgaagttcgg tttattgcct gggtaatgcg gatttggcac cagaactaat tgctgacctt
    12121 tttgccagct tttcagcata aatggtccgg aaccggcggt attttgcgcg aggaagccgc
    12181 gagcatcatc cgctgcatgt tcctttaaga ccgccggatt gataatggat gcaccgtcat
    12241 tcgccagcgt gtagaggaac ggtgcgaatg gttggctaag ggtaaacttc accgtatgtt
    12301 cgtcgggagc atcaatcttt aaatctttgg gaaatgcttc tgctggcccc tggccgattt
    12361 ttagtagccg ctcaaaagaa agttttactg cttcggcagt gacaggtgtg ccatcggcaa
    12421 atttagcatt atctttcagg gtgaacgtcc actctttttg atcgtcagac gctttccagc
    12481 tacttgccag atcgccttca acgtcggttg agcctttatc accgtccgtt ttgtgctgaa
    12541 ccagccgctg ataagacggg taggtcactg tccagtcgtt attatctatt gttaccgccg
    12601 ggtcgagggt ttgtggatcg gcggccttac caatcaccag catatctttt ggtacggcgg
    12661 cgtgcgcaac cgggaaattt gtggcaagga cgagcgcgag caatgtggga cgaaacgata
    12721 tcgatctctt catgacagtt tcctgattaa ctgacgtgct gtgtgccagg ggatataaaa
    12781 caagagaatt gatcagcgag cagagggtaa ctcgctgcct ggggtaattc gaagtgccac
    12841 cattcgctgg agataccgac aaaaccgcca ccagtcatta tcgcattcag caacagccga
    12901 ttgcgctgag cggcgggcgg gacggaaggg tgataggcat gggagcgctc gtgcatttca
    12961 tcgaaccctg cgcccatatc gaggatgttc ccgtgctcat cacgaagcgt caggtcgatc
    13021 gccgtgccac ggctgtgatt agaaccgacc gtcacatcaa caacatattg cgggtctggg
    13081 caggcttgcc acaacatcgc ctgtgcttgt tgtgggcgat acgcatcgta aatcaccagt
    13141 tgtaagcctg acagctgggc gatgctgata cttttcgcca gcgcggtaat cgcatccttg
    13201 tgtaacagac aacgcgcttg ctgataaata gctttacctg tgatgttatc agcgcaggcg
    13261 tatttcaatt cgatctccag atcagggaag attacggcta aatcaaccag ttcggtggta
    13321 tccgacataa acggttcctg tataagacaa aaattgctgc gctttccgct tatgcagatc
    13381 tcatgccatg ccgggagaag cgccagaatg ctggcttaaa gttatatttt ttaacttttg
    13441 atcaacattt gtgcagcgta gtgcagtttt ggtgcaagag gggaagttaa ggaaggaatc
    13501 tcccggaatc gtagctgaaa tcacagtatt taagtgacag tgtcacctta aaggaaaacc
    13561 cgcgagtgcg ggcgagagga atttgtcaga ttttcagcgg taacacgctg ctcatccagc
    13621 ctggaatttc ttcggcggga agggggcggg aaaagaaata tccctgaata acgcgacagt
    13681 ggatcttacg tagcatctca aattgctctt tggtttcgac cccttccgcc acgacggtta
    13741 aattgaggct ttgcccaatg ctggtaatgg cttcaagtaa ggccaggatg cgtttttccg
    13801 tcagacaacg atcgacaaaa cttttgtcaa ttttgatttc cgttaccgga agactgacta
    13861 agcgggataa tccggaaaag cccgtaccaa aatcatctac cgataagccc acgcccatat
    13921 cacgcaggat ctgaatgcgc ttaaagattt cggtatcgtg ttccatcatc atgctttccg
    13981 tgatttctac cgtcagctgg tggccgtcaa taccccaggc gtgcattgca tcagacacct
    14041 gattaggcag ttgattactg cgaaagtgca gtgccgacaa gttaacggaa agcgccggga
    14101 tatgaatatt ctggctacgc cattctgcta actgacggca agcttccgcg atgacccagc
    14161 gcccaatatt ttcgatttca ccaatctctt ctgcgagagg aataaaccgt gaagggggca
    14221 catgaccatg ctggggatcg tgccagcgcg caagggcttc gatgccgtac agttcacccg
    14281 tttctgcgaa gatttgcggc tggtaaacaa gtttcagttg gttattgcta atcgcttctt
    14341 tcagcgctgc gcctaaaacc aaacgctctt ttaccatttc gttcatcgcc gggctgaaga
    14401 actgccagcc gttaccgcca ttcttgcgaa tataatccat tgcattgtga gcagtggaga
    14461 gtaggtaatc gcggttttta cccacgtcgt agctgatgcc aatactcaag gtaagcggga
    14521 atggtttatc gtcaatcatt atcggcttgc tgaccacatt ccgtagctca tcggcgatct
    14581 gggtaatgtt actgacgtcg ttttccaggc tcacgaggac aaactgcgta ccttcgatac
    14641 gacagagata ctgatccggt ttgagttttt cacgaaagcg attgaccact tccagcaatg
    14701 cctgatcggc ccacgcatag ccaaggctat caatcacatc ctgaatatgg tcaacaccga
    14761 tgagatacac cacgggagag acggctttgt cgaccaggtc atcgaggtaa ttgtgcaggt
    14821 tattgcgatt tggcagaccg gtcatcggat caaattggat gagttgttca atatgctggc
    14881 ggcttttttc ctgttccagc gccagcgcgg ccatatgctg actgatatct gccacgcgtt
    14941 cgataaaggc gctggtttct gctcctgacg aggttttaat ttgcaggatc cccgcaggcg
    15001 cgccatcacg ctgacgaatg gtcgctgacc agctttgcgc attttgaact tctgcaccgt
    15061 gcgaagatga cgcccagtgt atcggcatcc cgttgcgcag tgcgaacagc gaaacatgcg
    15121 attcgttgag aacagattcg atgttacgac aaatgatttc ccccatttca tgaaatggcg
    15181 ggctgctgca catggcggcg agaatattgc cttcaagctg gcggatttgc cgttcttcgg
    15241 tgatatccga gaaagtcatt accaggttct gcagatgcgc gagcacgtca taaaccgggc
    15301 tgatagaggc tttaatccag attttttcac cggtgcgcgt caacagcaga aattcgtcct
    15361 gatcgcgggc ggttttccat agcaactgtt gtaaacgaat gcggttatcg gcagggaatt
    15421 caggaatgtt caggagtgta tcgggctgca taccgctggc ttcgctaatg cagtaaccaa
    15481 acatttcggt aaatgcgcga ttgcactgca caatatggcg ttccggatcg aggacaatca
    15541 ccggtcggtc gagatggtca acggcaataa tcaattgtcg agtctgttct ttttgcgcca
    15601 tttctacgct ggcatcccgt accagcgcca ggtaataaac tttcccctcg gcgctcactt
    15661 tcgatagcgc aaaacgggtc cagattttac tgccgtcttt tttctccagc tgcagctccc
    15721 gactcatccc ctcgacacgc gctttaccgc cttcacggtt atgacgaatg tattcaggat
    15781 gcgcaggacg caaatcccgc ggaatcagca tatcaatgtt attgccaatg acttcttcac
    15841 gtttgtaccc ccagagcttc tctgcggcgg ggttgaaaaa catcacttca tcattttcgt
    15901 taattaacac cgcacccatc atattttgct caagggcggg gaaaaaaatg ccatcggcgg
    15961 cattatccgc atcggttagc ttcatgatta cctctgcatc ctggcgcatc taaagactgg
    16021 ctttccagag ttcaacacgg tttctacctc gtcttttggc gatatacaga gcttcatcgg
    16081 ctatttgaat gaggcgctca tagtcaggat gaccattaaa catggcggca ccgatggaaa
    16141 gtgagagggc aatatcctcg ccgtttgcgg ctttcagttt ggttttctcc acccgactgc
    16201 gaatacgttc tgcggtacgt aacgtttcgt tttcagaagc ttcagtcaaa acaatgataa
    16261 attcatcgcc cccgtagcgg aaaacataat cactactgcg gacgttgtca taaaaggcct
    16321 gagagacttt acgcagaatt tcatcgccag tgttatggcc ccacgtatcg ttgatctctt
    16381 tgaatttatc aacgtcaata atcagcactg acagcggtgt accggtccgg ttggcatggg
    16441 caatttcgcg tttgaagata gtcggcagga aacggcggtt aagtaatttc gtcagtacat
    16501 ccataccgac ttcgtggcgc gatacttctt caaacaattc acgcagcaag gtaataattt
    16561 gcgatacggt atttcttatc tgtaatagaa acttcacccg caaacttctg ttattcaaat
    16621 tcctggtgtt acgcatggtt tgattgaaaa taccgtcgaa atcctgaatc agacgggaga
    16681 tatggcctac ttcggcaatg ccgctaaaat aatgtcgacc tttatggtta aaccacaggc
    16741 caaaatcagc ctggcttaaa ggcaaactac tgcctaaatc agaatccagc aggattttat
    16801 agataatatc tatttcccat gaaagtattg aggctatttg ccgttctttt tcttcttcgg
    16861 cgttttccag taacgagaag atacgatagt tttcatcttc ctttgaggca ctactgtcac
    16921 taaaggtaaa cgcgcgggtc atcacttcca tagcgatatc aatactgtta atcgagaaat
    16981 ggtagacctg aagtttttct gcggcagaat aatccgaaga gaagattacc ggatagagga
    17041 tttttttcag cacccgaaac cccatctcga caatttctac cggaattcct atgcgggcat
    17101 gcacttccgc gacggtatgc tggatttgta ttagcctttc gacatcgtca acctgggcag
    17161 aaagcacgtt aataatccag cgttccatcg cacttttcaa ctgccgctca acttgttcat
    17221 tactcaagaa ttcttcggca tgcgggtcga tgcggacaat tcgataaaac tcgatactca
    17281 gataatgagc atgcgcaacg gcaatttctg cggctttagc acgaatgaga ggatctgcct
    17341 gttcgacaag tccggtccac tcatctttca ttcttttaaa atacatctcc ataattcaca
    17401 cccttataag gctgggaaat cagacggaat caaaacgcaa cgtgcgagat cgactaactg
    17461 caccatattc tcctgaaata tgaagatata ctgaaaagaa ataagcgact taggacagtt
    17521 ttaatctacg ctactgttct tcagaagagt atagcccatc gtaattattt ttcggtgaca
    17581 gcgaatatcg tatggttttt catattcata catttttatt agggatttat agctgtttaa
    17641 ctaagtgtgg ttaattgcgt ggttaatttg acttatgtaa gcatgattac tagtgggata
    17701 tttcacaagg tgtaacaagc cggtgggtaa agcaccggct tgttacaaag taagcatggg
    17761 agtttaactg ccccaacgac tttgcagata gctgaccgct tgttgagtct gcggtttatt
    17821 cagatagtcc tcacggaaca agatggtgcc gctaatttct ggtaccgcat cgttaagatc
    17881 gagctgcttt ttcagttccg gtacgccgcc gttaatcatc cagtctggct ctatctttga
    17941 aggttcaccc actttataga aggcgatacc gatatacagg cgggtcctgg tcggtttaac
    18001 gacatccgcc caccattttg ccaacacgtc ataacgcgcg gcactccgtg agaacggcca
    18061 gtaaatttgg ggagcaatgt aatccagcaa tccttgttcc acccatcgac gggtgtcagc
    18121 gtaggattcg tcataggctg ccgcgcctcg ggtatcggaa ccgagcggat cgtgtgatcg
    18181 gttacgccac acgcctgccg ggctaacgcc aaattcgact cccggcttaa tgcttttaat
    18241 ggtgtgtgat acctttgcaa ttaactgctg agtattgttg cgccgccagt ctgcttttga
    18301 cgcaaatgcg cctccgtatt tacggtacgt ttcgttatca tttagccgtg aacccggtga
    18361 ctccgtatag aaatagtcgt caaactgcac gccatctacc ggatagcggg aaaccacttc
    18421 agcgactatt gatgtgatcc agtcctgaac ctcagggatg cccgggtcga ggacaaagcg
    18481 atcgccagac gttctgatcc agtcacggtg ttgcacatag acgctcgccg gttgttgaga
    18541 cagagtgcta ttcagttctc tgatagtacc gggcttcgta ttaaccgata cgcgataggg
    18601 gttaaaccag gcgtgtactt tcatcccacg cttgtgggct tcgtcgagca taaattgcag
    18661 cggatcgtaa cccggatttt caccaatctt accggtcata agatcggacc acggcaaaat
    18721 tttcgatggc cacagggcgg taccgtccgg cttgacctgg aaaaagaccg tgtttatgcc
    18781 gagacgttgc agatgatcca gtttgtcgat catcgcctgt tgttgtacac gggcccggct
    18841 ggtggggtta ctaatgttaa ccgaggaaac cggtggccag tcgaggcgag aaaccgtggc
    18901 cagccagatg ccacgcatcg gttgtgacga ttgttgcgtc gtggctggtg gctttgaacc
    18961 tgctggtggt gtcaccatgg actctggagg cgtgctttta caactacaca gtaaaagtgc
    19021 cagtgcaacc agtatcgctg gtcttctaat cgctaatttc tcgtttcggg agcagatatc
    19081 catatgtgct ggtttccggt aaacagatgt gcgctcattc tcgtacttat cccggttaag
    19141 tcaatacgac agcaagcacg aaaaagggag cgatgaatta tcgctccctt gtcttataac
    19201 cattcagaca tggttagtgt ttcttgtcat tcatcacaat atagtgtggt gaacgtgcac
    19261 gcgggtgcag gaagaagtga ccttttggtg cgttctgact gttgattggc tccagagtga
    19321 cgccggtatt cgctttgcct ttacgatcat gaacagcata gagaataaag ggcagggcaa
    19381 gtaccaccag gaaactaaca accagtaatt caacatacat atcggtagaa tcaccctgga
    19441 tgttatccgg cggcaggaag gaaacaataa acgccataat tgaagtcagc agaccgacaa
    19501 ttgccacgac cagtttcacc cctttaccac cagggatatt aaatgtgcgt tttaagtcag
    19561 gatgtttaag aaccaacaca atgtagccaa taaacagcat gaaataagca cacagataaa
    19621 tcaccaccgt cagcgccaga gcgatcagga aggacatgtt gttaccgcca ccggtattgg
    19681 tgaggatgat caacgcgata gaagtaatca ccagctgcga aatgaccagc gttaccggta
    19741 cgccattttt gttcatttta gcgaatgccg ctggcagcag gtttttctgc gctgtgacat
    19801 acatcccgcg agaaggacca acaatccagg aggcgatttc cgccagaaca cccagcaaca
    19861 gcagtgcgga gatcacgcga accgtccact caatttccgg tgccacatgg gacatcagaa
    19921 cggtaaaggt ttgcattacc cctgcggaga ggttgatttc attacccgga atgaccatcg
    19981 caatagacaa accaccaaca gagcttaagc agattgccgc caccatcagc agtaacatag
    20041 ccagtggata gtcgcgccct gggttactca tttcattgac gtgggttgcg gaagcttcta
    20101 cgcccatata actcaaaatg aaggcaacaa atacaaccag ggtgcccact ttagagaagt
    20161 cagggaagaa ggtcttcgaa tccatttcga tagcaacggg ggcaccggag tgcagataaa
    20221 tagccgctaa tgcgatcaaa ataaatgcag gtaacaggat accggcgaag aagccaactt
    20281 tagcaattcg cgccgtgtat ttcgtgccac caaactgcgt taatgccagc gcccaaagaa
    20341 tgatgagcgc tgcaatagtt ttggtaatgg ggtcttcatt cagcgctggc catttcagga
    20401 tgtaggagag tgcccctaac acgaaataga gcatcggaat aaaaccaatg gcgatttgca
    20461 gatagccaaa tgagatcgct gcaaatcccc atctcggccc cagagtattt gatacccagg
    20521 cgaagacacc accttcttcc cagccgtcga cggtggccat ttccgcagca caaagtccca
    20581 cgggaataaa ccataaaatc ccgcctaata gcaggaagaa gactaatgaa aagcccgatg
    20641 ttgcgaaggt agggtattca taaacagcca ttaccatcga tgccgttatg gcaaagaatc
    20701 cgagtaatgt gagctgctta gctttacctg tctgtactga tgtagccata ttatccccct
    20761 aaaacggtat tcctgtcgga accgcacctg ttttgttctg aagcgtattc agaacaatat
    20821 tttccgttgc taatgccagt gaacagactt tggaaattgt cccgaaacgg gttcgtttcg
    20881 ggacaccgtt accgttaaac gttatcaggt atgtttaaag ctgttctgct gggcaatacc
    20941 ctgcagtttc gggtgatcgc tgagatattt cagggaggct ttgtagtctt ccagcaacag
    21001 ttcagcaaag tccatttcga agccgcgacg acacataatg cgcatcacca cgatgtcggt
    21061 ggcttcaccg ccgagagtga aggccggaac ctgccagccg cgcagacgca gacgttcaga
    21121 gaggtcgtac agggtgtatc ccggatcttc accatctttc agtttgaagc aaaccgccgg
    21181 gatgccttcg tccgggcgac ccgtacagat gaactcatac ggccccagtt tggcgatttc
    21241 atccgccaga taagcggcaa cctggtaaga ggcgttctgt actttggtgt agccttcacg
    21301 accgaggcgc aggaattcat agtactgtgc aattacctga cccgccgggc gggagaagtt
    21361 gatggcgaaa gtaccaattt gaccacccag gtagtcaacg ttgaacacca gttcctgcgg
    21421 cagcgcttct tcgtcacgcc agataaccca gccgcagccc agcggagcca gaccgaattt
    21481 atggcctgaa gcactgatcg atttcacacg cggcaggcgg aagtcccaga cgatatccgg
    21541 ggcgacgaac ggtgccagga agccaccgct ggcagcgtcg atgtgcatgt cgatgtcgat
    21601 accggtatcg gcctggaatt tatccagcgc atcgtgcagc ggttgtggga actcatagtt
    21661 accagtgtag gtcacgccga aagtcggcac cacgccgatg gtgttttcgt cacaggcttc
    21721 aatcatgcgt ttcgggtcca taaacaactg accggggcgc atagggatct cacgcagctc
    21781 cacatcccag tagcgggcga atttatgcca gcagatttgt accggaccgc acaccaggtt
    21841 tggtttatcc gttggtttgc ctgcagcttc catacgcttg cgccaacgcc atttcatcgc
    21901 catcccgccg agcatacagg cctcggaaga accaatggtg ttggtgccaa cggcctgacc
    21961 atttttcggc gcaggcgcat gccacagatc ggcaaccata tttacgcaac gcaggtcgat
    22021 ggctgcggat tgcggatatt cttctttgtc gatccagttt ttgttaatgg ataaatccat
    22081 caatttgtgg acattttcgt cgtcccaggt ctggcagaaa gtggccaggt tctgacgagc
    22141 gttgccatca agatataatt cgtcattgat aatctggaat gcgacatcgt cgcgcatttc
    22201 gtgcagcgga aaacgttttg attctgcgat agtggaaata gacttcgcac caaaacgtga
    22261 atcgagtagt tccgacctta aatccgttac ttgcttctta tccattttaa actccttaaa
    22321 atgattggat cgcattaaaa aagtaggatt tatcgataaa gtaagcaagt gcaaaggact
    22381 cgtgtttaaa taacaaa
//
LOCUS       DAPGWD010000063        22161 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPGWD010000063 DAPGWD010000000
VERSION     DAPGWD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.063"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..442)
                     /locus_tag="RHX88_002393"
     CDS             complement(<1..442)
                     /locus_tag="RHX88_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDV2231070.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLP"
     gene            complement(701..1954)
                     /gene="amiC"
                     /locus_tag="RHX88_002394"
     CDS             complement(701..1954)
                     /gene="amiC"
                     /locus_tag="RHX88_002394"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDV2231071.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2186..3517
                     /gene="argA"
                     /locus_tag="RHX88_002395"
     CDS             2186..3517
                     /gene="argA"
                     /locus_tag="RHX88_002395"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDV2231072.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3579..5405)
                     /gene="recD"
                     /locus_tag="RHX88_002396"
     CDS             complement(3579..5405)
                     /gene="recD"
                     /locus_tag="RHX88_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDV2231073.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5405..8947)
                     /gene="recB"
                     /locus_tag="RHX88_002397"
     CDS             complement(5405..8947)
                     /gene="recB"
                     /locus_tag="RHX88_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDV2231074.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARSVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTRPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQASLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8940..11828)
                     /gene="ptrA"
                     /locus_tag="RHX88_002398"
     CDS             complement(8940..11828)
                     /gene="ptrA"
                     /locus_tag="RHX88_002398"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDV2231075.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKR
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQVPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12004..15372)
                     /gene="recC"
                     /locus_tag="RHX88_002399"
     CDS             complement(12004..15372)
                     /gene="recC"
                     /locus_tag="RHX88_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDV2231076.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSAKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLDSSQELDAFVDVMPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPNSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLTCNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15385..15708)
                     /locus_tag="RHX88_002400"
     CDS             complement(15385..15708)
                     /locus_tag="RHX88_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDV2231077.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15693..16100)
                     /locus_tag="RHX88_002401"
     CDS             complement(15693..16100)
                     /locus_tag="RHX88_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDV2231078.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIADYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16097..16660)
                     /locus_tag="RHX88_002402"
     CDS             complement(16097..16660)
                     /locus_tag="RHX88_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV2231079.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(16651..17121)
                     /locus_tag="RHX88_002403"
     CDS             complement(16651..17121)
                     /locus_tag="RHX88_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV2231080.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17306..18100)
                     /gene="thyA"
                     /locus_tag="RHX88_002404"
     CDS             complement(17306..18100)
                     /gene="thyA"
                     /locus_tag="RHX88_002404"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDV2231081.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18107..18982)
                     /gene="lgt"
                     /locus_tag="RHX88_002405"
     CDS             complement(18107..18982)
                     /gene="lgt"
                     /locus_tag="RHX88_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDV2231082.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19133..21379)
                     /gene="ptsP"
                     /locus_tag="RHX88_002406"
     CDS             complement(19133..21379)
                     /gene="ptsP"
                     /locus_tag="RHX88_002406"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV2231083.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21392..21922)
                     /gene="rppH"
                     /locus_tag="RHX88_002407"
     CDS             complement(21392..21922)
                     /gene="rppH"
                     /locus_tag="RHX88_002407"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDV2231084.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT         5340 a         6116 c         5474 g         5231 t
ORIGIN      
        1 ccggtagttg aacgctaaat tgctcattaa tgattgttaa tgcatcacga cttattgcat
       61 actctgcttt gtacaacgct ttgatttcct caaggaaaac attattaatt tgtatgcctg
      121 agacgactcg ggatattgca ttcgaaatgt gatcagcaag aatcagtagt agcgatggat
      181 tgagtttttt ttcgagatta ctctccgcat aatgcactat cttttcggca acaaaaatat
      241 actcaatacc cacatgctca atcaatttgt agagtttatt tttttgctca tttctgacat
      301 aaaaaatccg gtttgcagga tgctcaggga ccgacatacc atatttttta ttgtaaccaa
      361 cgccaggccc agaaatgata acctcctgtc cgttcaccga tgcctgtaca caattattgt
      421 tcatgacttt ttcgatgatc attccatggc tcccaaaaaa aaagcaaggc atgtcagaag
      481 taataattac ttcaaacatt gccttgcctg atttaacagt tacaagataa ttaactaatg
      541 cgtatacctt acacggaatt gtgcttaata aaagagatga caggcttatt ttttaagccg
      601 atattcaaat attcgacagc agtcacaaaa ccatcgttca acatttatgt aaataatatt
      661 tattcaatga aacataaatt cattgaataa attaaaaaca tcatcccctt ctcgccagcg
      721 tcgccccatc ggcaaaatac gctttaatcc ccgcaagaat agactctgca acttcctgct
      781 gaaaggtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
      841 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacctgat
      901 ttttatgcag cttgttgatt ttaccgagct tattcagtac cgctttacca aacttaaggc
      961 tgtcagcaat ggtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
     1021 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
     1081 ccgcagtact ggttgcacct ttggttgaga gcgcaaacac agaggaaccg ctcggctgac
     1141 gactggtaaa ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt
     1201 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
     1261 tattgccctc tttctcgatc agagagcgca aacggcgagc tatttgcaat accacatctt
     1321 tttctcgcgt tttgtatttc cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa
     1381 gcataatgac aatcggacga tcgcgccctg ctttacctgg ttgtggacca ctttgcgctg
     1441 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
     1501 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
     1561 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatgc
     1621 gtacggtctg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
     1681 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
     1741 ccacgcgttc cgggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
     1801 ccgtcacacg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctgaccg
     1861 cagccagact gacctgactt acgctcaata gccacatggc acccgcgcct tgcagtaaac
     1921 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
     1981 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
     2041 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
     2101 tctcgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata cactaatttc
     2161 gaataatcat gcaaagaggt gtgccgtggt aaaggaacgt aaaaccgagt tggtcgaggg
     2221 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
     2281 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt
     2341 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
     2401 tctggctgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
     2461 aacactggaa ctggtgaagc aggctgcagg aacattgcaa ctggatatta ctgctcgcct
     2521 gtcgatgagt ctcaataaca cgccgctaca gggcgcgcat atcaacgtcg tcagtggcaa
     2581 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
     2641 tatccggcgg attgatgaag acgcgatcca tcgtcaactg gacagcggtg caatagtgct
     2701 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aacctgacct cggaagagat
     2761 tgccactcaa ctggccatca aactgaaagc tgagaagatg attggttttt gctcttcaca
     2821 gggcgtcact aatgacgacg gtgatattgt ctcagaactt ttccctaacg aagcgcaagc
     2881 gcgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttacg
     2941 tggcgcagtg aaagcctgcc gcagcggcgt gcgtcgctgt catttaatca gttatcagga
     3001 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
     3061 ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
     3121 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
     3181 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
     3241 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
     3301 ccgcagttca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
     3361 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
     3421 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
     3481 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaatcg ccggatgcgg
     3541 caggcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
     3601 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
     3661 tcggcataca gcgacagacg gcgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
     3721 gttactaccg gcgtgcgttg gctcggtaaa atcaatgccg catggtcgaa ctccgatccc
     3781 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
     3841 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
     3901 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
     3961 accggtcggc cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
     4021 ataaactgct caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
     4081 cacaaaagtt ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc
     4141 agcagatcca gataacgtcc ataacccgca agagcttcct caagcatcgc aatataatct
     4201 tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
     4261 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
     4321 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
     4381 ccggttcctg ccggaacgtg agtccccgtc aggcggctta gctgcctggc acgctcggcg
     4441 gtaaagcccg cgttggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
     4501 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatcgggcaa ggcgtcgatc
     4561 agtctcgaca tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
     4621 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc gcccagcaat
     4681 cggtgcaaag tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
     4741 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
     4801 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagtaacttc
     4861 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
     4921 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
     4981 tccagggttt gcgccagtag agcttcatca acctcaatgg catgattcac ctcattgaag
     5041 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
     5101 aggatcatcg gcgtgggttc atctcccctg ctgactgctt gagaagccag caagcattct
     5161 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg atgcgacgcc
     5221 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
     5281 aacagtgccg ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaat
     5341 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
     5401 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc
     5461 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca
     5521 ggaacagata gataacgccg ccaaagtgac gatcatagtc gtaatcagca atgcgatggc
     5581 gcagataacg atgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
     5641 gcatcgctgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
     5701 atttatagtc gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt
     5761 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa
     5821 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcgctaatc ggcaggtaaa
     5881 actccatctc cacctgttta tcgcggtcgg aaagctgact caggctaaca cccgtttcat
     5941 tgagagatgc ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt
     6001 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc cgggtaaaat
     6061 cgaggtcttc aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat
     6121 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
     6181 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga
     6241 cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag
     6301 catcattaac ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat
     6361 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
     6421 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
     6481 ggcgcaccag cggtgcaacg ccgagactac aatgccaaac cgaacgcgtc agcgccacgt
     6541 aaagcaaacg cagatcttcc gccagacgtt cggcctccgc gagatcgaca ctttctggcg
     6601 cagcgttaag atccagaaca gcctcaaacg agtgtcgatc gtgataaaac gcctgatcct
     6661 ggacgcggaa attggtaata aatggcaacc agaccagcgg atattccagc cctttcgatt
     6721 tatgtatcgt gacaatctgc accaaatgtt tgtcgctttc gaggcgcatt tgttggctgg
     6781 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
     6841 tttccagctg cgttccggct tcttgtagca gttcgctgat atgcaagata tcggtaagac
     6901 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
     6961 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
     7021 cgactaccgc atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
     7081 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
     7141 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
     7201 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
     7261 cctggcggct gcgcaccagc acgctgatgt ccgaagcacg caccgaacgc gcgtcgtcgc
     7321 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
     7381 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca
     7441 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
     7501 tcccggctga tttcactgga ataaacggta tttcgcgaaa catgaacgcg tcatcagtct
     7561 ggctgaaaag cttattcacg ctgttcacca ttcctggtgc ggaacgccag ttggtgtcta
     7621 aagtgtagtg ggcgtgaact tcgctacgcg ccttcatata agtgaagata tccgcacccc
     7681 ggaatgcata tatggcctgt ttcgggtcgc caattagcaa caatgcggtt tccggctgat
     7741 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
     7801 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcaccg ctttcgctac
     7861 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
     7921 tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg ggtgatcacc agatcgcgga
     7981 tcgacaatgg ttctgcaagc agttgatcga tcgcctcaaa cagtggatgt cgcggggttt
     8041 cccccccggc tttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactctg
     8101 gcaactgata actgttggtc tcttcttctg cccaggcgct gatcttttcg atccatttag
     8161 cctgattact acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
     8221 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg
     8281 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcgcttcgc
     8341 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
     8401 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
     8461 cctggtagcg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
     8521 caaaggcatt caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt
     8581 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
     8641 cttccagcag gcgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
     8701 actcgtggat attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg
     8761 tgaccaccag cagttcttca acggtcagcg ggcggggaaa ggcggcggaa ccgcctagtc
     8821 caagtaacag gcgcaaataa agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
     8881 cttcaatcag gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
     8941 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
     9001 actttccagc cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc
     9061 gacagaatag ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
     9121 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
     9181 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtacctgc
     9241 agcatctggg taattaccgc ctgctggatt tgcgcaaact cttctggctt catcgcccgc
     9301 aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa ggaaggctgt
     9361 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaat
     9421 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
     9481 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
     9541 acaaataccg ctgccagtgc ggagtcggtg ctgttaccag ctttttcaaa gatgacggat
     9601 tgttttttat cgaccacgac atctttgtta cgacaccact ctgaaccatc agcgcccaac
     9661 tgtttttgca catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc
     9721 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
     9781 gtaatggagg gcaaaatttt gcgccgttca tctcgcgaga agtacggcac ttgcgagagc
     9841 atctgcgcgg gcataatcgc ctgctcgaac gctttgccct tttctgcgga atccatcatc
     9901 tggttatacc aggacttcgc ctgctcaagc tgatcttccg tggcggtata gctaaagtag
     9961 ccttccagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaacc
    10021 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    10081 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    10141 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcggcttt gggctcgctg
    10201 gcaaaataac ggcttggcgc atacactact cgcagattcg actcatcaac aatcagctct
    10261 ggatggtcgt atttcttctc tgacttaatc agcgagaaat cgtcaggaat ataagggtta
    10321 agctctggca aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt
    10381 gcgctgattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
    10441 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc
    10501 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca
    10561 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac
    10621 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg
    10681 cctttttcac gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta
    10741 gccaggcctt tatcggttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    10801 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcagaa
    10861 agtgtacctg ggctgcgatt gccaatcaga taggtaatca attcatcggt tttactacgg
    10921 aacttcgctg agttgttatc gatgcgaaac tcaacgcgca acactttacg cggcagcgca
    10981 gggacgtaat gaatgataat gcgcttttgc gcgtcggtga ctaccggcac ggtgatttcc
    11041 ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    11101 tccggcagcg gtttattact gtaaataacc gccttcatca aattggcgga atagtacttc
    11161 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg
    11221 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc
    11281 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt caccgcatta
    11341 cgctcacgtt cggcatattt cttgtcgagc aaaggttcag caatagcatc ggccaggcgg
    11401 tctaccgcac caggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    11461 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    11521 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    11581 tacgcctcgg gatcttccag cgacccaacg ggcaccacca gcgccgagag cgatttaact
    11641 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    11701 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    11761 gcctgactta agggtgccca aagggcaact aacaacaata atgctttgaa ccaggtgctg
    11821 cggggcattc acggacctca taatcaactt aattttctgt ccagattcaa cacgttaacg
    11881 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
    11941 tcagtccgtg actggtgcgc atcataaagt aagcggatag attgcgcaat ttttatacag
    12001 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    12061 ggcctccatt gtctctggtg ttaattgccg ccagagcctt tgataccaga tatcatcacc
    12121 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
    12181 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    12241 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac atcccttcac gatacccctc
    12301 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    12361 gccgtctttg cgtagaaaaa ggcgactttc accattacca ccgctggcac agtagacaag
    12421 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    12481 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggtgag
    12541 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    12601 catctcctgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    12661 tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa
    12721 taactgctga ttgatttgat aacggctaag cccttccaga ataaatggct cggtgtctgg
    12781 gatttcgctg tcttcagtac ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
    12841 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    12901 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    12961 acgagcatag ctttgtcgtt cgcctggctg gtagttttgc ggatcaaacg gcatccgggt
    13021 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    13081 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    13141 cgggaaacgc tcactgttat cctgaatgga acgaccgata tagctgatat agagtttttg
    13201 ctgcgcggaa attaacgctt ccaggaacag atagcggtcg tcatcgcgac ggctacggtc
    13261 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    13321 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    13381 cagagtacaa atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    13441 acgctgtgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    13501 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    13561 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    13621 cagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    13681 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    13741 ccactcgccc tgcgcgctct ccatcgcgta gcccaacaac atacgcgtca ggccaaatcg
    13801 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatgcc
    13861 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    13921 gtcaaaccgc gccgccagca ccggcacatc cagcaacgcc agcacatcct ctgacacaaa
    13981 acgactgtta ggcagtgata acaggctgat aaacgcttcc agtaccggat gtgactgccg
    14041 cgcacgacgg tcggaaatgg cgtaaggtag gtaacgatcc gcaggtgcgc taccaaacac
    14101 agcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    14161 agtaagtgtc gggtcttcct ccagcatcgc cagcaggcga tcgtgtaaaa cttcaacttc
    14221 acgctgcggg ctatggcaaa cgtggaaggt gatactgcta tccagtggat caagcgggcg
    14281 tttgttatcg ctacgggaaa actcttcgat gttcacacca gcaacggcgc ggttttccag
    14341 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcatcacat cgacaaaggc
    14401 gtccagctcc tgactgctat ccagatcgga aaggagatag atatagtcgc gccccaactt
    14461 gccccaggag gccagtagcg ggttgccgac atcctgttca ccgtcgctgt taaagagctg
    14521 tccggcattt tcgctatcgc gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    14581 ctgacgagtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    14641 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg
    14701 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
    14761 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tggcgcggtg
    14821 ccagcgcggt tgcccgagtt gatgggtata ttccaccaac gccttccaca acggcgcttg
    14881 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
    14941 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgcag aaagctggaa
    15001 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    15061 atccagcaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    15121 gctctctttg gggatttccg gtaacacccg gacgaacata tcccagataa agctcgcagg
    15181 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    15241 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    15301 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    15361 gacccttaac atagcggctc ctgactactg acgattcggg caatgcaggc gggtcatctc
    15421 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    15481 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
    15541 ctgccagcca tgccgccaga gctgttggta ctggtttctg ctggcaagac tgttcattaa
    15601 tgttcgctgg aaacccgata acgcagtgac aatcatcacc atcaacacca tcgccaacat
    15661 tacctccggc aggctaaagc cttgttgatt cttcagggaa gctgacataa cgccctctct
    15721 ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa caatgtttcc atcaatgact
    15781 tcgcctgttc gccacaacga aacgccttca taatcggcaa tcaataaggc ttcattatct
    15841 gccagtaaac gcaaacaaac ctgggcatcg gttccagcgt actgcgagca ctgaactgct
    15901 ggctgcgtct gccaggagtg catttttccc cacgccagcg ccgactgaac gatggcctgg
    15961 cggcgcaatg actgactttc catgctcacg cgagaggcaa aactgcgatc ctgttggctc
    16021 attccttgta atagcaagct acctaaaacc agcagcatca ggaccagagc cagtgacgaa
    16081 acaccctttt cgcggttcac aggttgaatc ctgtcacgct atagctggca ttcaccacgg
    16141 tttgcggttc agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc
    16201 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    16261 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    16321 tcagacgaaa tccaatctgg tcagactctt ttaccggttc gcgatcccag ataccgttac
    16381 tgttcgcatc ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc
    16441 cggtacagct gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    16501 gcagccagat ttcatcttcc agcgccagct tacgggtgct cgttaaactt tcacgctgta
    16561 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    16621 acacttccag cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg
    16681 acaaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    16741 gaagcgaata tgcccggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    16801 caggtcgctc atttcgactt cgggccagcg tggcacaaag accaatggtg aactgccatg
    16861 acaggtattg gccccagcag cggaactcac aaggcaccat aacgtcccct ccctgataac
    16921 gctgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    16981 ccgcgcctgg ctggcggttt gccatagccg ttgcgactgc tgccagtatt gccagccata
    17041 gagtccactt gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt
    17101 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    17161 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa
    17221 tttcgggaag gcgtctcgaa gaatttaacg gagggtaaaa aaaaccgacg cacactggcg
    17281 tcggctctgg caggatgttt cgtaattaga tagccaccgg cgctttaatg cccggatgtg
    17341 gatcgtagcc ttcaatctca aagtcttcga aacggtagtc gaagatggat tcgggtttac
    17401 gtttgataat caacttcggc agcggacgcg gttcgcggct taattgcaga tgagtttgat
    17461 ccatatgatt gctgtacagg tgcgtgtcgc cccccgtcca gacaaaatcg cccacttcca
    17521 gatcacactg ctgcgccatc atatgcacca gtaacgcgta gctggcaatg ttaaacggca
    17581 ggccgaggaa aacgtcacag gagcgctgat aaagctggca agagagtttg ccgtctgcca
    17641 catagaactg gaagaacgca tggcacggtg ccagcgccat tttatccagt tcgcctacgt
    17701 tccacgctga aacaataatg cggcgcgaat ccgggtcgtt tttcagctgg ttcagtaccg
    17761 tagtgatctg gtcaatatga cgaccatccg gcgttggcca ggcgcgccac tgtttaccat
    17821 acactggccc gaggtcgccg ttttcatcgg cccattcgtc ccagatggtg acattgtttt
    17881 cgtgtagata agcaatgtta gtgtcgccct gcagaaacca cagcagttca tggatgatgg
    17941 aacgcaggtg gcaacgttta gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca
    18001 tctgatgacc aaaaatggaa agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt
    18061 cgtcgagcac tttttgcatc agttctaaat actgtttcat ggttcctcag gaaacgtgtt
    18121 gctgtgggct gcgacgatat gcccagacca tcatgatcac acccgcgaca atcatcggga
    18181 tggaaagaat ttgccccatg ctgatgtact gcacccaggc accggtaaac tgcgcgtcgg
    18241 gctggcggaa aaactcaaca atgatgcgaa acgcgccgta gccaatcagg aacaagccag
    18301 agacagctcc cattgggcgc ggtttacgaa tatacaggtt gaggataata aacagcacca
    18361 caccttccag cagcagctcg taaagctgtg atgggtggcg cggcagcaca ccgtaagtgt
    18421 cgaaaatgga ttgccactgc gggttggttt gcagcagcaa aatatcttct gtacgggagc
    18481 ctgggaacag catggcaaac gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa
    18541 taaagttgcc cagacgcccg gcaccaagac caaacggaat gagtggtgcg ataaaatcag
    18601 aaacctggaa gaaggaacgt ttagtacggc gggcaaagat aatcatcacc acgataacgc
    18661 caatcaggcc gccgtggaaa gacatgccgc cgtcccagac acggaacaga tacagcggat
    18721 cggccataaa ctgcgggaaa ttgtagaaca gaacataacc aatacgtccc ccgaggaaga
    18781 cgccgaggaa gcccgcatag agtaagtttt caacttcatt tttggtccag ccgctgcccg
    18841 gacgattcgc ccgtcgtgtt gccagccaca ttgcaaaaat gaaacccacc agatacatca
    18901 ggccgtacca gtgaagcgcc acgggtccta ttgagaaaat gaccggatca aactccggaa
    18961 aatgcagata gctactggtc atctgtcacc acaagttctt gttatttcgc tgaaagagaa
    19021 cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa
    19081 ggggacctgg ctggttgccg gataccgtta aaagatatgt atatgatccg cgctataacc
    19141 ctccgcgaat cagcccgccc atgccgcgac gctccataaa ggctgcaacc tgatggcgaa
    19201 cttcggtcgc cagttgcgct tccagactac gctgcgcaag attttctgct tcggcataat
    19261 caatgcgccg cagcaggtat tttacccgcg ctacagaacg tccgttcata gacagatggc
    19321 gatacccaag cccaatgagg attgccacgc acatgggatc gcccgccatt tcaccgcaca
    19381 aacggagatc gattccatgt atttccgctt cccgggcgat catcgccaga gctcgtaaca
    19441 ttgcaggatg aagactgtca taaatgttcg ccacccgggt attgttgcga tcaacggcca
    19501 gaatgtattg agtcagatcg ttggtgccaa cagagatgaa atcgacccgc tttgccagat
    19561 gcggcagcat aaataccatt gacggcactt ccagcatgat gccgatacgt ggtttgggaa
    19621 tttcgtaacc gatcatctcc tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt
    19681 catcaacttc atcgaggctt gtgaccatcg gcaacagaat attcaggttg cccgtagcgg
    19741 cattagcacg cagcatcgcc cgcacctgga tcaagaagat ctccggctga tcgagcgtaa
    19801 tgcgaatccc acgccaaccc aggcatggat tctcttcgct gatcggcatg taaggcagct
    19861 gcttatctgc tccgacatcc agcgtacgca aggtgacggg tttatcattg aacatttgca
    19921 gcatcccctg atactgcgcc acctgttctt cttccgacgg gaaaccactt tgcagcatga
    19981 atgggatttc agtgcgataa agaccgatgc catcaatacg gctgcccagt ttttcttcat
    20041 gttccgggct taaaccagca ttgagcatga cttttatacg ctcaccgctt tttaactgcg
    20101 cgggtaaatt gacgtcatct tccgccagac ggctaagctc aatctcttca ctaattagcc
    20161 gctgatattc ttgcagcagt accggctccg gatcgaccag caattcaccg cgatagccat
    20221 caacgatcag cgtcctacga tgcagcaccg aaggctgaat atccgcgccc atcacggtag
    20281 ggatccccag cgcacgtacc atgatcgcag catgggagtt ggctgcgcca tctcgcacga
    20341 caacaccgac taagcgatcc tggggcagct cagcaagagt tgtcgctgac agttcatctg
    20401 ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg attagcgtca tcaagatgaa
    20461 acagcaatcg ctgacccagc gcacgtaaat cgccagcccg ctctttgaga tagttatcgc
    20521 ttagcgcggc aaactgttcg gcaaattttt caatgaccgt ttttaccgcc cactctgcca
    20581 ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag ccgggtgtcc gaaagcaggt
    20641 gcgagtaaag atcgaaaata gccgccgttt ctttttgtgc accggcggca aagcgtttgc
    20701 tgtagcggcg aaactcgttt gccgcttctt ccagcgcccc ggtcagtcgt tcgcgttcca
    20761 gagccggatc cagcgttgat gcctgataca cctgttccat taaaggtaac gtggcatcct
    20821 gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa tgcgcggatt cgcgtctggc
    20881 gatattgccc aaacagggca gtcaactgcg actgagaaag aatagctgcc atctgggtgg
    20941 caagcgtcac caggaaggat tcttcacttt cgtcatactg gcgcaactct cgttgctgta
    21001 ccaccagtac accaagcaac tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga
    21061 aacgttcttc ttttacggag gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa
    21121 ggtttatcgg ttccgccagc ctgccaacca ggccgacgat cccttcatca aacgcgagcg
    21181 ttacagtgcg accgcgtggt tttttcagcc cccgggtcgc catcaggtag taacaacgtc
    21241 gatcatgatc ggccaggtag accgaacaga cctcggtatc catcgcaaga cagatgtcgg
    21301 taaccagaat atttaacgcc tcattcaggc gtggtgcgct ggctaccttt tcgactattt
    21361 cgcgcaggcg agtgagcata attggcgtga cttaacctct tttacgtcga taagcagatg
    21421 cgttttgtgg ttttggcgta ttttcctgca gtgacatcac cacactcgcg aactctttca
    21481 ttaccctacg gtagacatca cgtttaaatg acaccacctg tctgaccgga taccagtaac
    21541 ttacccatcg ccagccgtca aactctggtg tactgctggt ttgcatattg atttctgcat
    21601 cgccgctcac cagctgcaag agaaaccatt tttgtttttg gccgatacaa accggcttcg
    21661 tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa
    21721 ggattcgaac gtctttgcgg cttaatccta cttcttcaaa caattcacgg tacatcgcct
    21781 gctctgcgga ttctccgggg ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa
    21841 atcgccgggc ccacattacc tgcccctggc gattacaaat cacgatacct acgtttgggc
    21901 ggtagccatc gtcatcaatc accggactac ctcaaaataa agctttatat acgaatgatt
    21961 gtttcatact ccaggaagac ggtaaaccac tctctgcagg gcattacaca ctaataacaa
    22021 ttgaataact cacagttatg tgcagagtta taaacagagg aaggggtgga tagccgtttt
    22081 tattcacctt ttctgtggat agagttgtga agaactacgg aattactacg ggaaaacccg
    22141 gagcattctg aataagcccg a
//
LOCUS       DAPGWD010000064        21305 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPGWD010000064 DAPGWD010000000
VERSION     DAPGWD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.064"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..668
                     /locus_tag="RHX88_002408"
     CDS             <1..668
                     /locus_tag="RHX88_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV2231085.1"
                     /translation="ARILNSIWLITALGLVTTAGLAWVAKYLEITATKYDSPQMYVAI
                     GLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAPVPAIIVVFSTAGAMFL
                     ISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERPVWVISCLMIVLWSGII
                     SHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFGILAAIAITLVWQRHTR
                     FFH"
     gene            complement(704..1660)
                     /locus_tag="RHX88_002409"
     CDS             complement(704..1660)
                     /locus_tag="RHX88_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDV2231086.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1660..2901)
                     /gene="clsB"
                     /locus_tag="RHX88_002410"
     CDS             complement(1660..2901)
                     /gene="clsB"
                     /locus_tag="RHX88_002410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDV2231087.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2898..3659)
                     /locus_tag="RHX88_002411"
     CDS             complement(2898..3659)
                     /locus_tag="RHX88_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV2231088.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3792..4202
                     /locus_tag="RHX88_002412"
     CDS             3792..4202
                     /locus_tag="RHX88_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDV2231089.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4164..5270)
                     /locus_tag="RHX88_002413"
     CDS             complement(4164..5270)
                     /locus_tag="RHX88_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2231090.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5281..6414)
                     /locus_tag="RHX88_002414"
     CDS             complement(5281..6414)
                     /locus_tag="RHX88_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2231091.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6407..8143)
                     /locus_tag="RHX88_002415"
     CDS             complement(6407..8143)
                     /locus_tag="RHX88_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2231092.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPLVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8136..9131)
                     /gene="hlyD"
                     /locus_tag="RHX88_002416"
     CDS             complement(8136..9131)
                     /gene="hlyD"
                     /locus_tag="RHX88_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDV2231093.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(9134..9805)
                     /gene="cecR"
                     /locus_tag="RHX88_002417"
     CDS             complement(9134..9805)
                     /gene="cecR"
                     /locus_tag="RHX88_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDV2231094.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10034..11398
                     /gene="rhlE"
                     /locus_tag="RHX88_002418"
     CDS             10034..11398
                     /gene="rhlE"
                     /locus_tag="RHX88_002418"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDV2231095.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11630..12112)
                     /gene="ybiA"
                     /locus_tag="RHX88_002419"
     CDS             complement(11630..12112)
                     /gene="ybiA"
                     /locus_tag="RHX88_002419"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HDV2231096.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGHGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12232..14382
                     /gene="dinG"
                     /locus_tag="RHX88_002420"
     CDS             12232..14382
                     /gene="dinG"
                     /locus_tag="RHX88_002420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDV2231097.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14410..15372
                     /gene="ybiB"
                     /locus_tag="RHX88_002421"
     CDS             14410..15372
                     /gene="ybiB"
                     /locus_tag="RHX88_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDV2231098.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15513..16598
                     /gene="hcxB"
                     /locus_tag="RHX88_002422"
     CDS             15513..16598
                     /gene="hcxB"
                     /locus_tag="RHX88_002422"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDV2231099.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(16827..17087)
                     /locus_tag="RHX88_002423"
     CDS             complement(16827..17087)
                     /locus_tag="RHX88_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2231100.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17352..17618)
                     /gene="ybiI"
                     /locus_tag="RHX88_002424"
     CDS             complement(17352..17618)
                     /gene="ybiI"
                     /locus_tag="RHX88_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDV2231101.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17692..18369)
                     /gene="ybiX"
                     /locus_tag="RHX88_002425"
     CDS             complement(17692..18369)
                     /gene="ybiX"
                     /locus_tag="RHX88_002425"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDV2231102.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18411..20693)
                     /locus_tag="RHX88_002426"
     CDS             complement(18411..20693)
                     /locus_tag="RHX88_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDV2231103.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(20958..21218)
                     /locus_tag="RHX88_002427"
     CDS             complement(20958..21218)
                     /locus_tag="RHX88_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2231104.1"
                     /translation="MKKCLTLLIATVLSGIFLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
BASE COUNT         4941 a         5573 c         5754 g         5037 t
ORIGIN      
        1 aagccaggat attaaacagc atatggctca ttaccgcttt aggtttggtg acaaccgcag
       61 gactggcctg ggtagcgaag tatctcgaaa ttacggcaac aaaatatgat tcaccgcaaa
      121 tgtatgtcgc cataggatta ttattacttt gtatgtatgg cttaagtaag gatatcaaca
      181 agataaatgc cgtcatcgca ggcgtaatat atctgttttt actctctttg gtggcgattg
      241 tcgttgcaag tttagctcct gtacctgcca ttatcgtcgt gttcagcact gcgggcgcga
      301 tgtttttaat cagtatgctg gccggtttat tatttaatgt tgatcctggt tctcaccgtt
      361 ttatcattat gatgacgttg acagggttgg ccctggtgat catcatgaat gccgcattaa
      421 tgagtgaacg acccgtttgg gtaataagtt gcttaatgat tgtgttatgg tcaggcatta
      481 tctcgcatgg acgaaataag ctccttgaat tggcggggaa atgccatagt gaagagttgt
      541 ggagtccggt tcgttgcgct gttacaggtg cattaacact ctattactat tttatcggct
      601 tctttgggat acttgccgcg atagctataa cgcttgtctg gcaaaggcat acgcgttttt
      661 ttcattagtc gtaaatgcag atactaaaga acttaactaa acttcacatc gccgcttcat
      721 ttttcgcccg tagcttcttc gcctggcttt ccagtagcag gtaacagatc agcgccagca
      781 gcagcgggat aaagtaatac aggacgcgat aagcgagcag ggcggcgata atgctccctt
      841 tcgaggtatg ctccccagcc agtagtgcga taaacaccgc ttccagcaca ccgatccccg
      901 ccggaatatg cacgatgacg ccagcaatac tactaaccag taacacgccc agtacaaaga
      961 aatagttcac gctttgacca agtaacagcc agataatcgc ccccattacc atccagttaa
     1021 cgctggaaat cagcatctga gcgagggcga atttccatga aggcagcacc agcttttgcc
     1081 ctttgatggt catatggcgg tgcttcgcga aagcgcaaaa ccacaaataa accgcgataa
     1141 tcatcagtaa gccaatgccg agaatacgca gcgtagtttg atcgacatac cagtgatccg
     1201 gcaactccac cacgcctgcg gtaaagataa tccctgccag taaaatatag cccagccagt
     1261 tggtggtaat actgagcgag aaaatccgcg taatagtgct gcccggtaac cctagtcggg
     1321 agtacaaacg atagcgcata ccaatgccgc cgacccaggt actgagcgtc aggttgaagg
     1381 cgtagcagat aaacgacacc agcatcacct ggcgcttcgc cagtttgtga ccgcagtaaa
     1441 agcgggcgag caggtcatag cagccgtaaa tcagatagct gacgaccacc agcccgaccg
     1501 cactaagcag cgcaacgcga ttgtagtcgc ggatgacctt ccagacctct tcccagtcca
     1561 cttttttggc gtagaccacc agcaacacaa tcaccgcgat aaaaaacagc caggtgagga
     1621 tcttctttgc taagcgccag cgcgggtgtg atttactcat cagggtttta cccccgtgtt
     1681 ttcagtttct acccgatcct gcgtttccat tgtcggttgt gcgggcggat caacctgcgc
     1741 cagacgtggt gtgtgtgccg gaagccagcc aaccagcgcc gggaagtggc gtaaaaagtg
     1801 gaacgccagc acgcttttgg tcaggttcca ccaggtgcgt ttcggcagca tggtctcatc
     1861 gacctgctga caatctgcgg caataatgcc gttcagatta tcgcgcagcg tctggttaaa
     1921 atgacgatcg tggatgatga catttgcttc gagattcagt gacaaactga gcggatcgag
     1981 attactggac cctactgtcg cccagtgatc gtccatcaat gccactttgc cgtggagcgg
     2041 gcggcggcgg tactcaaata cctgaacgcc gcctttaacc agatagttat acagcaagcg
     2101 cgcaccgact ctgacaatcg gcatatccgg ttcgccctga atgatcagtt tgatccgcac
     2161 cccgcgccgt gccgctttac gcaaggcgtg taaaaatcga tagccgggga agaagtaggc
     2221 gttggcgata atgacttctc gccgcgcctg agtgagcatt ttcagatagt gacgttcaat
     2281 atcatcgcga tgttcttcgt tatcgcgcca gaccagcaat acctgcgctt ctcccggctg
     2341 gcggttctct tccgctttgt gatgacgtcg ccaccagcgt cgtgccgcgc tctgtccagg
     2401 caggttttcc agctcaaact ggagaatatc ttcgacaatc ggcccttcaa ggcgtaccgc
     2461 gtaatcctgt ttagcctctg gaccgtagct ggacatatgc tcggcggagt aattcagccc
     2521 gccaataaag gcgatacgcg cgtcgatcac cacaattttg cgatgcatcc ggcgaaacac
     2581 attggtgcgc ataccaaaaa ggcgagggcg gggatcgtag tagcgaaata ccacgccagc
     2641 tgccgtcagt tcattgacaa actcatcgct gagatccggc gaaccgtagc catccagcaa
     2701 gacttccgct ttaaccccgc gttgcgctgc tgccagtagt gccgcatgca gctgtttgcc
     2761 gacgtcatcc tcaaaccaga taaacgtttc aagaatgatg cgttcttgtg cctcgccaat
     2821 cgccttaaac accgcgggat aatattgctc gccgttttcc agcaactgga tcttattgcc
     2881 ttcgcgccag ctacatttca taaatgaatc tccgcactta aaggggcatg atcagaaagg
     2941 tgtcgccatg tccgcagcgg caacgcggtt ggcgcgctgg cgctggcatt tttgacgtag
     3001 atcctgtcca gtcgtagtag aggaaattgc accggaaacg tgcgcgccgg gcgtccgtgg
     3061 gcgcgggtaa aaatctcatc cagtccggcc tgcactttta acggatgatt agctttttgc
     3121 cgccagtcat tgaaatcacc cgccaccaat accggttcgc cgtccggtag ctcattcacc
     3181 cattcggcga gcatcgcaag ctgcgcctga cggtgcgcct cacgcaggcc cagatgtacg
     3241 cacatcacat gaatcgcttt tccggtcatc ggtggcacaa tgcggcagta gagcacgccg
     3301 cgcttttccg caccatcgac cgaaacatcg cgattctcat aatgttcaat gggataacgc
     3361 gacagtacgg cgttgccgtg atgcccttcc gggtatacgg cattgcgacc gtaggcaaaa
     3421 tcgctccaca tcgtgtcggc gagaaactcg tagtgcgagg tatcgggcca gttttccaca
     3481 tgcagcggat gaacttcgtg cgcgcccatc acttcctgca ggcaaacaat atcggcgctg
     3541 acggtacgca ccgcgtcgcg aagttccggc aaaatgaagc gtcggttaaa cgcggtaaaa
     3601 cctttgtgaa tgttgatggt gagcaccttg aacgaaaatt gttgtgtttg atcgggcata
     3661 attttcctgt ctttgcctct tatctcattg aaatagtgta gtcggcgtca caaaaaggtg
     3721 cggtcttacg gaattttccg taaagttcgg tactctgagt aagtagagat aaattcttca
     3781 ggagagaagc catgaagtgg caacaacgtg ttcgtgtcgc aactggtcta agttgctggc
     3841 agattatgtt gcatttactg gtagtggcgc tgctggtggt gggctggatg agtaagactc
     3901 tggttcacgt cggcgtggga ttatgcgcac tgtattgtgt cacggtagtg atgatgttgg
     3961 tgtttcagcg ccaccccgag caacgctggc gtgaggtggc agacgtgctg gaagagctga
     4021 ccacgacctg gtattttggc gcagcgctga ttgtgctgtg gctgttgtcc cgtgtactgg
     4081 aaaacaactt tttgctggca attgcagggc tggcaatcct tgccggcccg gcggtagtgt
     4141 ctttgctggc gaaagataag aagttacatc accttacgtc taaacatcgc gtacgccgct
     4201 gaccctgtcg tggccgttat caccagtagc ggccacaaac tattccacac aatatccaga
     4261 ctcgcatcct tcaaataaat ctgcttggta atgtccgtaa agtggcgaat agggttaatc
     4321 cacgtcaggt tttgcagcca tactggcatg ttttccaccg gagaaacgta accggaaagg
     4381 agaatggcgg gcatcataaa aacaaacacg ccgataaacg cctgctgttg tgtcgagcag
     4441 agtgatgaaa tcaacagacc gaatcccacc agcgataaac cgtaaatcac catcgtaaag
     4501 tagaacagcg ccagcgatcc ggcgaagggg atttgatacg cccagatacc aatcgccagc
     4561 acaatggtgg cctggaaggt ggcgacaatt aacgccggta cggctttgcc gatgaagatc
     4621 tgccaggtgg tgagcggcga aaccagtagc tgatcgagcg taccttgttc acgttcgcgg
     4681 gcgacggaaa gtgaagtgac gatcattacg ccgatagtgg tgatcatggc gatcagcgac
     4741 ggcaccacaa accatttgta gtcaagattc gggttatacc agttgcgtac caccagctcg
     4801 ctgttgttag gtttcggttt tccttccagc agctcctgct gataattttt gacgatctgt
     4861 tgcaggtagt tggcggcaat ttgcgcactg ttggagttac gcccgtcgag gatcaactgc
     4921 aacggcgcgg tctggaaggt atccagtttg cgcgagaagt cagccgggaa acgcaccagt
     4981 agtaacgcct tttgtgtgtc gatggttggg cggatctcct gtgggctttt cagcagcagc
     5041 acatgagtaa aggcgctggc gcgggcaaaa cgttgggtca gctccaccga atgctcgccg
     5101 ttatcttcat cgtagatggc gatggtggcg ttagtcactt ccagcgtggc ggcgaacggg
     5161 aacagaatca cctgaataag cacaggtaaa atcagaatcg cgcgggtttg cggttcgcgc
     5221 agcagcgact gcaactcttt gcggattaac gtccataagc gatgaaacat gctcttctcc
     5281 ctaatccagc cgacgtttgg ttttcagcca cgtcaggccg ataaacatca ccgccgaagc
     5341 gatcaaaaac agcacgttta ccaccagcac cactggaata ttcccggcga ggaacaggct
     5401 ttgcagggtg ctgacgaaat aacgagcggg aataatgtac gtcaccgcgc ggatcaccgc
     5461 gggcatactg tcgatctgaa aaataaagcc ggaaagcata atcgacggca gaaaagcggc
     5521 gttcagggcg acctgagcgg cattgaactg gttgcgggta atcgtggaaa tcagcagccc
     5581 catccccagg gtactgagta aaaacaggct ggagataaaa aacagaatca gcagcgaccc
     5641 gcgatacggc acgccgagaa taaacactga caccagcata cacagcaaca tcgccagcat
     5701 cccgagaaag taataaggga tcagcttaca cagcagcagt tccgtgcggg taatctccgt
     5761 agagagcaga gcctccatgg tgccgcgttc ccattctcgc gccaccacca gcgaggtgag
     5821 aatcgcgccg atgaccgtca tgataatggt caccgcaccg gggataatga agtgctggct
     5881 aatcgccgcc gggttaaacc agtagcgggt ttgtacatca ataagcggtt caaaagtctg
     5941 cccgttgtcc tccgctcgct gcatttgcca gatctgccag atcccttcga catacccctg
     6001 tacaaagtta gcggtattcg gctcactgcc gtcggtgatc acctgaatcg gtgcggtggc
     6061 gttggcgcgc tccatctgtt cggcaaaatc caccggaata accaccagac cgcgaatttt
     6121 ccccgcctgc attttggcga tcagttcctg acggttatcg ctgatggtgg cgtcgatgta
     6181 gggcgaaccg gtcatggtgt gggtgaaatc cagcgccgct tcgctacgct gttccagtaa
     6241 aatcccgacc cgcagcttgc tggagtcgag gttaatgccg taaccaaaaa taaacagcag
     6301 caacagcggg atcactaccg caatcagcca gctactcgga tcgcgaacga tctgccgcgt
     6361 ctctttgacg cacagcgccc gcacgcgacg ccaggaaagg atcgggttac tcattgctat
     6421 gctccttatc ccagtcgtgg atcaactgaa taaaggcttg ctccatggtg ggatcgggtt
     6481 gctcgtcgtt ggccgactgc gctttcaaat catccggcgt gccgctggca attaatttcc
     6541 cacggtacac caggccgatg cggtcgcaat attctgcttc atccataaag tgggtggtga
     6601 ccatcaccgt gacgcctttc tctaccatgc tgttgatatg tagccagaat tcgcggcgag
     6661 taatcggatc aacgccggac gtcggttcgt cgagaaacag aatgtccggt tcatgcatca
     6721 gcgagcaggc cagtgccagc cgctgtttaa aacctaatgg cagttcatcg gtggcgtggg
     6781 aggcgatact tttcaggccg aacgcctcgc tcatacggga gattttttcg ttctgcgccc
     6841 gaccgcgtaa gccatacaca ccagagaaaa agcgtaaatt ctgttcgacc gtcaggttac
     6901 cgtagagcga aaacttttgc gccatatagc cgagatgctg gcgcgcttta ccagaactct
     6961 ctttcagatc catccccagc accagcgcct ggccggaagt cggcaccagc aaaccgcaca
     7021 tcatcttaaa ggtggtcgat ttacccgcgc cgtttggccc cagcaaacca aaaatctccc
     7081 cacgtttaac ggcaaagttg acgtgatcgg tggcggcaaa atcaccaaat ttcttggtca
     7141 gttctttcgc ttcgatcacc gtctcgccgg gtgtgccttc taccgtatgt aatattgcgc
     7201 ccagcggcga ttctgaggtt ccggcaccac ccagcaaatc aataaatgca tcttcaaaac
     7261 gcggcgtagt ttcgttgatg ttgatttccg gcatcccgtc ggcatggcga atatcgtctg
     7321 gtgtggcctc ttttttgagg atcagacgta ccgatttccc ctgaatcatg ccgtcgctga
     7381 ccagtggcag tttcaaggcg cgttgcaata gtttgcggtt gccctcgtgt ggactggtca
     7441 tcagaaaact gcgtccggcc atggtttgtg tcagggcttt tggttccccc tgatacagca
     7501 actcgccttc gttcatcagt aacacgtcac ggcactgctc ggcttcgtcg agatacgagg
     7561 tactccagag gattaacatc ccttcgcctg ccagctcatg caccatctgc cacagttcgc
     7621 gccgtgagat agggtcaacg ccgacgccgg gttcatcgag cagcaacact ttcggttcgc
     7681 ccaccagggt acaggccaga ccgagttttt gtttcatccc accggagagc ttgcccgcca
     7741 ggcgtccggt aaacggccca agagacgtaa actccagcag gcgagcaaaa gtttgcttac
     7801 gtgcctcgcc ggtgacgctg cgcaaatccg cgtacagatt gaggttctcc atcaccgtga
     7861 gatcttcata cagaccaaat ttctgcggca tatagccgag cacggcgtgc agcgcgccgt
     7921 cgtttttgat tggatcaaag ccaatcaccg tggcattgcc gctgtcgggt ttcagtaatc
     7981 ccgccagcat ccgcattagc gtggttttac ctgcaccatc cggccccacc agccccgtca
     8041 cataaccggc gtgaatggta caatcgagcg gcgcgacggc gggcttgtcc atgcccggaa
     8101 agcgtttttc caggccgttc agcgtgataa cggcatcatt catgtcctgc ctcgttaccg
     8161 aattgcaccg tcaccggcat tccctggcgt aacgcatcat cggcgtcggt caccacaata
     8221 cgcaggcgat agacgaggtc ggtacgcaga tccggtgttt cgacggtttt cggggtaaat
     8281 tcagcagtcg gcgaaacgaa accaatctgc ccgtgatagg gcttgtccgg gcgaccatcg
     8341 gtataaagca gcactttgcg ccccggctgg gcctggtcaa gattccgttc atcaacatag
     8401 gcacgtaccc acaccggacg cgttagtgaa acggtaaaca ccgtgccacc ttcattgaga
     8461 accgtgcctg gctccaccgc gcgcgttaac agcgtgccat cagacggggc tatcaacgtt
     8521 gagtcctgca aattcaactc cgcctgcgcc agttgcgcct gcgcctgttc gaggctggct
     8581 ttcgcctggg cgatgtcctg ttcacggtta ccggaacggt actgacgcaa tttatcctgt
     8641 gctgatttca gcgttgcctg cgcctggtcg cgcgaggagc gggcattttc caggtcgttt
     8701 gccgaaatgg tgcggctttt ccacaaccct tgctggcggt tatagaagtt ctgcgcatag
     8761 tcataggcgg cttgcgcctg tttcaccgct gcggcggcct gagcgatttc ttcatcgcga
     8821 tacccggcaa gcatcaggtc atactgcgcc tgtgccaccg aaacacccgc tttcgcctgc
     8881 atcagggcaa tctcatacgg cttgtgatcc agttcgccca gcacctggcc cgctttgata
     8941 gcatcacctt cgtccaccgc cagcgattca acgcgccccc caacacggaa actaagattc
     9001 accgtacgaa tatccacgtt gccatacagc gtcaggccgt tatcctggcg gctttgatac
     9061 caccagtagc ctccggcaac cacggcggca agtaccacta ccgccagtcc gatcacgaca
     9121 ggttttttca tcactacaaa ctcctttgcg ataatccttg caaaatcagg tcgatatgac
     9181 aggtcaccgt ctggttgatc agttcggtct tttcttcatc gaacgcggtc cagccggtac
     9241 gtaacagaat tgtttctttg ccaagacgga acgccagaat ctcaccaatc aacgcatgag
     9301 tatggaggat catgcgggta tcgttggcgt cgcagccggt ccaggcggca atcaggcgtg
     9361 tcaggtggct gtgtagcggg ctaatcacct gttcgtgcac caggtgatag gctgccgtgg
     9421 gagagagctg ctcacgggag ataaacttgc tgaggttgac ggtgtcatcc tgggtgagca
     9481 gcttaatcat gttcctgcag gcgcgaagga tcagttcacg gatggctgcg cgatcaggct
     9541 gtggttgtgc gaacaagcgt tcggcctctt cggcgtgcgg acggaactgc tcgccaataa
     9601 aatcggcaat ccactgggcg caggcgaggt acaaatcttc tttcgaaccg aagtagtagg
     9661 tgatggcagc gatattctgc ccggcctggg cggctatctc gcgggtggtg gcgttcattc
     9721 catattcacc aaactgcgcc agtgcggcag caatcagctg ttttttcgcc tgttcaccct
     9781 tgattgtcat ggcaggatta ttcatcgcac agtccattct taatcaaatg attgattaag
     9841 attatgactc catatgggag ttgtccagta tggctaagaa ttttagctac gccagtcaca
     9901 gggataattt atgcgctgcg tcacaaaaac tgctacactc cgctccctca tgacattgtg
     9961 gtttttgtca ttttcctttt cagtatctcc ctgaaaacta caccggtaac ggtcggggcg
    10021 gttcggagta gttatgtctt tcgattcttt gggtttaagc cctgatatcc tgcgcgccgt
    10081 tgccgagcag ggttaccgtg aacccacccc tattcagcag caggcgatcc ctgcggtgct
    10141 ggaaggccgc gacctgatgg ctagcgccca gaccggtacc ggtaaaacag cgggctttac
    10201 gctgccgctg ttgcaacacc tgatcactcg ccagccgcac gccaaagggc gtcgtccggt
    10261 acgtgcgctc attcttaccc cgacccgtga actggctgcg cagattggcg aaaacgtccg
    10321 tgattacagc aaatatttga acattcgttc gctggtggtg tttggcggtg tcagcattaa
    10381 cccgcaaatg atgaagctgc gcggcggcgt tgatgttctg gtggcaacac cgggacgttt
    10441 gctggacctg gaacatcaaa atgcagtgaa gctggatcag gttgaaatcc tcgtcctcga
    10501 tgaagctgac cgcatgctcg acatgggctt tatccacgat atccgtcgcg tgttaacaaa
    10561 actgccagcg aaacgccaga acctgctgtt ctctgcgacc ttctctgacg atattaaagc
    10621 cctggcggaa aaactgttgc acaacccgct ggaaatcgaa gtggcacgcc gcaataccgc
    10681 gtctgatcag gtgacccagc acgttcactt tgtcgataag aaacgcaaac gcgaattgct
    10741 gtcgcacatg attggtaaag ggaactggca gcaggtgctg gtgtttaccc gtaccaaaca
    10801 cggcgctaac catctggctg agcagctcaa taaagatggc atccgtagtg cggcgatcca
    10861 cgggaataaa tcgcaaggtg cgcgtacccg tgcgctggct gattttaaat cgggtgacat
    10921 tcgcgtactg gtggcgaccg atatcgctgc gcgcggtctg gatatcgaag agttgccgca
    10981 cgtcgtcaac tatgaactgc caaacgtacc ggaagattat gtccaccgta tcgggcgtac
    11041 cggtcgtgcg gctgctaccg gtgaagcgtt gtcgctggtg tgtgttgatg aacacaaact
    11101 gctgcgtgat atcgaaaaac tgctgaaaaa agagatcccg cgcattgcga ttccgggcta
    11161 tgagccggac ccgtcaatca aagccgaacc gatccagaac ggtcgccagc aacgtggcgg
    11221 cggcggtcgt gggcaaggtg gtggtcgcgg tcaacagcaa ccacgccgtg gggaaggtgg
    11281 cgcaaaatct gcaagcgcga aacctgcaga aaaaccgtct cgccgcctcg gcgatgccaa
    11341 accggcaggc gaacaacaac gtcgccgccg tccgcgtaaa cctgccgctg cgcagtaatc
    11401 ttttatgccg ggctatgccc ggcatcaggc tgatgaacaa acgcaaaact gcctgatgcg
    11461 ctacgcttat caggcctacg tgaactctgc aatatattga atttgcatgc ttttgtaggc
    11521 cggataaggc gtttacgccg catccggcat ttcacaacaa gcacttgtca gcaatttgag
    11581 aacacgggaa aaaatttatc tgttttaccg cccataagcc accgagacgt tacttctcta
    11641 tagccaattg ttcgcgcaac tccattaaaa ggtagcccag tcgattcttg cccttaccat
    11701 gaccaccgtc tccccagtaa gcatcgtttt ccgtatgctc aaccagtttt gcgggcgcgg
    11761 ttgccagcaa gagcgcacgc agttctgcat gctgttcgaa tttagcgcga agtgcttttc
    11821 gcatcacctg ttctttgacc gactcccagt ttttacgcag aggcttagaa cgatcgcgcc
    11881 ccatgcgtgc ggcgaccata ggggaagaaa cccgacggat ctcttcgcgg tatttttcat
    11941 caaggaattt ttgtgcctga aaatagtgtt ctgaggtggg ccaggttttc ccgtcaacct
    12001 tgatgggcca ggcggcaaag ttagaaaaat caccgtagtc atcgctggtg ctgtagaaat
    12061 ttatgatggt gtcttgcatg acgtgttgga tcctttgtgc tcgaacgggc attaaaccgc
    12121 attatgttgg tggttattgc gagccgcttt ccagaaacag aaaaaccatt acccctgaaa
    12181 accgaaaaat gccacaatat tggctgttta tacagtattt caggttttct catggcatta
    12241 accgccgcgc ttaaagcgca aattgccgcc tggtataagg cgcttcagga acagatcccc
    12301 gactttattc cccgtgcgcc gcagcggcag atgattgcgg acgtcgccaa aacgctggcc
    12361 ggagaagaag ggcggcatct ggcgattgaa gcccccaccg gcgttgggaa aacgctctcc
    12421 tatttgatcc ccggcatcgc cattgcccgc gaagagcaaa aaacgctggt ggtgagtacc
    12481 gccaacgtgg cattgcagga tcagatttac agcaaagatt taccgctgct gaaaaagatc
    12541 attcccgatc ttaaattcac tgccgctttt gggcgtgggc gctacgtttg cccgcgtaat
    12601 ctgacggcgc tcgccagcac tgagcccacg caacaggatc tgctggcgtt tcttgacgac
    12661 gaactgacgc caaataatca ggaagagcaa aaacgttgtg cgaagctgaa gggcgatctc
    12721 gacacttata aatgggatgg tctgcgcgat catactgata tcgctataga tgacgatctc
    12781 tggcgtcgtt taagtaccga caaagccagc tgcctcaacc gcaactgtta ctactatcgt
    12841 gaatgcccgt tttttgttgc tcgtcgggag attcaggaag cggaagtggt ggtggcaaac
    12901 catgcgctgg tgatggcggc gatggaaagc gaagccgtac tacctgaccc gaaaaatctg
    12961 ctgctggtgc tggacgaagg tcatcacctg ccagatgtgg cccgggatgc gctggagatg
    13021 agtgccgaaa tcaccgcgcc ctggtatcga ctacagctgg acttgttcac gaaactggtc
    13081 gctacctgca tggagcagtt tcgcccgaag accatcccac cgctggcgat ccctgaacgt
    13141 ttgaatgcgc attgtgaaga gttgtatgag cttatcgcct cgttaaacaa cattctcaat
    13201 ctctacatgc ctgccgggca ggaggcagag caccgttttg cgatgggcga actgcctgat
    13261 gaagtgctgg agatctgcca gcggctggca aaactcaccg agatgctgcg tggtctggcg
    13321 gagttatttc ttaacgattt aagtgagaaa accggcagcc acgatattgt gcgtctgcat
    13381 cggttgattt tgcagatgaa ccgcgcgttg gggatgttcg aggcgcaaag caaactctgg
    13441 cggctggcgt ctctggcgca atcttccggt gcgccggtga ccaaatgggc gacgcgggaa
    13501 gagcgcgaag ggcagctgca tctctggttt cactgcgtgg ggatacgtgt cagcgatcag
    13561 ctggaaaggc tgctgtggcg cagtattccg cacattattg tcacctccgc aaccttgcgt
    13621 tcgctgaaca gtttttcgcg tttgcaggag atgagcgggc tgaaagagaa agcgggcgac
    13681 cgttttgtgg cgctggattc cccctttaac cactgcgaac agggcaaaat tgttattccc
    13741 cggatgcgct ttgagccttc catcgacaac gaagagcagc atattgccga aatggcggcc
    13801 tttttccgtg agcaggtgga gagcaaaaaa catctcggta tgttggtact gtttgccagc
    13861 ggacgggcga tgcagcgctt tctcgactat gtgacggatt tacgcctgat gctgctggtg
    13921 cagggcgatc agccgcgtta tcgcttagtt gaactgcacc gcaaacgcgt tgccaacggt
    13981 gagcatagtg tgctggtggg cttacagtca tttgccgaag ggcttgattt gaaaggtgat
    14041 ctgctcagcc aggtgcatat ccacaaaatc gcttttccgc ccatcgacag cccggtggtg
    14101 atcaccgaag gggaatggct gaaaagcctc aaccgctatc cgtttgaggt gcaaagcctg
    14161 ccgagcgcct cgtttaacct gattcagcag gttgggcgac tgattcgaag ccacggttgc
    14221 tggggcgaag tggttatcta cgacaaacgc ttgctgacca aaaattatgg caagcgacta
    14281 ctggatgcat taccggtatt tccgatagag caaccggaag tccctgaagg tatagttaaa
    14341 aagaaagaaa aaacgaaatc cccacgccgt cggcggcgtt aatgatgtga gtcaggtaag
    14401 gagtcgtaaa tggactatcg caaaatcatt aaagagatcg ggcgcgggaa aaaccacgcg
    14461 cgcgatttag accgggatac tgcgcgcggt ctgtatgccc atatgctcaa tggtgaagtc
    14521 cctgaccttg agttgggcgg cgtattgatt gcgctacgta tcaaagggga aggggaagca
    14581 gagatgctcg gcttttacga agccatgcaa aatcacacca tcaagctgac gccgcctgca
    14641 ggcaagccga tgccgattgt catccccagt tacaacggcg cgcgtaaaca ggccaacctg
    14701 acgccgttgt tggcgattct cctgcacaaa ctcggttttc ctgtggtggt tcacggggtt
    14761 agcgaagatc caacccgcgt gctgactgaa accatttttg aattgatggg tattacgcca
    14821 acgttgcacg gcgggcaggc gcaggcgaag ctcgacgaac atcaaccggt gtttatgcca
    14881 gtcggcgcgt tttgcccgcc gctggaaaaa caactggcga tgcgctggcg gatgggcgtg
    14941 cgtaacagtg cacataccct ggcgaaactg gcgacgccat ttgccgaagg tgaggcgctg
    15001 cgtctttcca gcgtttcgca tccggaatac attggacgcg tcgcgaagtt ctttagcgat
    15061 atcggcgggc gggcgctact aatgcatggt actgaaggtg aagtgtatgc taatccgcag
    15121 cgctgcccgc agatcaatct cattgaccgt gaagggatgc gggtgctgta tgaaaaacag
    15181 gacactgctg gtagcgagtt actgccacaa gcaaaagatc cggaaaccac ggcgcagtgg
    15241 attgagcgtt gccttgctgg cagcgaaccg attcccgaat cgctgaaaat ccagatggct
    15301 tgctgcctgg tggctacggg tgaagcggca actatcagcg acggcctggc gcgcgttaat
    15361 caggcatttt aattcttttc tcagccggat gagccatgtc atccggcttt taccccaccg
    15421 caatatgaaa ttcctgcatc tttattgacc ttcccacgcc cggcgtgcag cataaaaaaa
    15481 caacaaacac ataacataaa caggagttaa ccatggaaag tggtcatcgc tttgatgctc
    15541 agacgctgca cagttttatt caggctgtat ttcgtcagat gggtagcgag gaacaagaag
    15601 cgaaattagt ggccgatcat ttaatcgcgg caaacctggc agggcatgat tctcatggta
    15661 ttggcatgat cccaagctat gtgcgttcct ggagtcaggg gcatctgcaa attaaccatc
    15721 atgccaaaat cgttaaagag gcgggggcgg cagtcacgct cgatggcgat cgcgcattcg
    15781 gtcaggtcgc ggcacacgaa gcgatggcgc tggggattga gaaagcgcat cagcacggta
    15841 ttgctgccgt ggcgctacat aactcgcatc atatcggccg tatcggttac tgggcggagc
    15901 agtgtgcagc ggcggggttt gtctctatcc actttgttag cgtggtcggt attccaatgg
    15961 tcgcaccgtt ccacggtcgc gacagccgct ttggcaccaa tccgttctgt gtggttttcc
    16021 ctcgtaaaga taatttcccg ctgttgcttg attacgccac cagcgccatt gcatttggca
    16081 aaacccgcgt cgcctggcat aaaggcgtcc ccgtgccgcc aggttgcctg attgacgtta
    16141 acggcgtgcc gacgaccaat ccggcggtaa tgcaggagtc gccgttgggt tcgctgttga
    16201 cctttgccga acataaaggc tacgcccttg cagcgatgtg tgaaattctt ggcggggcgc
    16261 tttccggcgg taaaacgacg catcaggaaa cgttacaaac cagtcccgat gccattctta
    16321 actgcatgac cactatcatc atcaacccgg aactcttcgg cgcgccggat tgtaacgcgc
    16381 agaccgaagc ctttgccgag tgggtgaaag cctcgccgca tgatgatgat aagccgattt
    16441 tgctaccggg cgagtgggaa gtgaacacgc gtcgcgaacg gcagaagcag gggattccac
    16501 tggatgcggg aagctggcag gccatttgtg atgcagcgcg gcagattggt atgccggaag
    16561 agacgttgca ggctttctgt cagcagttag ccagctaaaa aaaagcccgt ccagtggcgg
    16621 acgggcaaac aagggtaaca taggatcaat gagggttaga gcatatgcgt ctgtcggcaa
    16681 acagacaggg aaatacttgt gctggacgta gcgtaaacgc ctgatccgtc caacggttcg
    16741 gattttgtag gcctgataag acgcggtagc gtcgcatcag gcatctgttg ccggatgcgg
    16801 cgtaaacgcc ttatccgtcc tacgaattac ttataaatta ccgcagtacc gcttaattta
    16861 ttgttgttgg tggcggaagt gatgctgtat ccactggcac cggctgccgc ggctttctca
    16921 gccagtttcg cttccagggc atcgagggtg gatgcgccat cggcagaaac cacgccgatt
    16981 ttattcatgt tctgtgcctg ggatgccgtt accggttccg cggcaaacac gccaaatgac
    17041 agagttgaaa gagccatagc agcaacaaca gtattgatag ttttcataat taatctctcg
    17101 caggtgattt ttgtataagt gacgttgttt cgtcgatgtg atgagtatca cgttttttta
    17161 cgagagataa aatcgaagag aattgaccgc cttgttcaaa taaattgatt gatatctaac
    17221 atactgataa accatttatt tgttgtactt atggacttgt tttatgccgc gtggcagtca
    17281 cagcagcgtt aaaggcacgg aatgccacgc aaaatacaaa ttatactcgc tatgcatctc
    17341 gccagttgta gttaacgtaa ctggctgtct ttcgaacctc tgcgattata tcctgtatac
    17401 gctggttttt gtaaatcttt ctcctgctga caatgaatgc ataagcgcac gccaggaatg
    17461 gcttcccgac gggcttgcgg gatgggggca ccgcactctt cacattcatc caggctttcg
    17521 ccacgcggaa tttcaccccg agcgcgggca atcgcatctt caattgtact gttgatctgt
    17581 tcgttgacgg cgtcatcgtt agcccaaccg gatgccatat cgtcctcccc atatcaatgc
    17641 ttgtacagtt aagtgtagct aatccaggga cgaacttggg cagttcaagc atcagatctc
    17701 cgaccattcc cgcagcagat tatgataaag attaagcagc gacaggatct cttcactttc
    17761 gccgtagcgg cttttcagcg actgaatatt gttgtccagt tcaaacagca tggcgcgctt
    17821 tttatcatca cggatcatcg actggatcca cataaatgat gccactcgta cgccgcgggt
    17881 tacgggtgtc acgcaatgca ggctgctgga gggatacaac acgagatcgc ctgccgggag
    17941 ttttacccga tgttgtccga aggtgtcatt aacgaccagt tcgccgccgt cgtagctttg
    18001 tggatcgctt aaaaacagcg tggcagaaag gtcagtacgc atccagccgt tttgtggatg
    18061 gctgcgtact gcgccatcca catggaaacc ataggtttca ttgttctgat agcgattaaa
    18121 cagcggcgtg gaaagggtac gcggcaaggc cgcggcaaag aacaaagcat gttggttaac
    18181 cgcgttcagc acctcatttt gcagggcggc gtataacgtg ctgcgggtgt cgacctgttg
    18241 attgttctta acttgcgcac cttgtgcgcc ggtggtgacg cgtccatcca cccattcggc
    18301 ttgttccagt tgttcgcgaa accgggcgac gtcctgtggc gataacacgc cgggaatgtg
    18361 gtacatcata caagttctcc aaaaagtggg gcctgcgccc cacatctgaa tcagaaatgc
    18421 atattggctg tgagcaagaa ggttcttggc tcgcccgggt gataacggta gccgctcttg
    18481 ttgattgagg cgacgtaatc agtatcaaac aggttgtaga cgtttagctg gaagtcgaga
    18541 ttgcgattaa cccgataccc cagtttggca tcggcgaccc agtaaccttc ggtaaatgct
    18601 ggcgttccca ccgcgccgtc tgaaccttta tgcatactgc cgatatagcg tgcgcccgcg
    18661 ccaacagaga tatcgtcggt tgcctgatat tggctccata aggtgaaggc gtgctccggg
    18721 gtatacggca gcgatgagga accatcctgg gcaacatctt tgccgttttt gatggttgct
    18781 ttttgctggg tatagccgcc aatcacctgc cacgcgggag tgatattccc ggccacggat
    18841 atctcatagc cttcgacgcg tttcttaccg tattgcgagt aagttccgtc atcattttgc
    18901 tcaacttcat tttcgatatc agtacggaac agcgcggcgg tgagcaacag acgtttatcc
    18961 agaacctgcc atttggtgcc aatctcgctg gtgttggctt tttgcggttt aaaatcggtg
    19021 cggttggcac tgttaccgct gccagactgc gcaagggcga agttgttgcc gcccggaggc
    19081 tgctgggaaa cggcatagtt aatatagaca ttaccgtttt ccgtcaggtg atacagcgcc
    19141 ccggctttcc agttcaccag attgcccgac ttggcggtgt cgacggtggt gaccggagaa
    19201 ccttttgcca caccagctgg gcaggtgatg gcaccgcgtc cgctgccgcc gcaggcggtg
    19261 gcactgtcat attcagtatg ataattatcc agacggatcc cgccgttcag ctcaaaatca
    19321 cgggtgattt gcagcgtatc gaaggcgtaa attgcgaagg tatccgtctg accattggcg
    19381 tttgcgccgt tgcgcgtcag gccgccggga tgaatgctgc tgtcaggatg ataaatattt
    19441 accgcgggta acgtcaccgg attaacgccg tagttagtct gcgtttcacg ggtaaattcc
    19501 acgccggtac tgacatcatg accgatagaa ccggtataga acgtcgaggt caggttggtc
    19561 tggttggtga gaattttatt actcacatct ttggtattcg ccgtgcgtga ccaggtccag
    19621 ctattcacat cgctggtggg ctgagtaata ttcgacgccc cgcccataat cgccgtcatc
    19681 aggtaatcct gctttacgcg cgaccaacgg gtagtattgc gaatggtggt gttatcgttg
    19741 atgtcgtgct caaaacgcat ggtggcggtg tcggtggtcg aatcgtcgta atcggaatcc
    19801 gtgccgtaaa agttatgagt atcaactttt ccggaatgat tcagagccgc tgttcccgca
    19861 gatggggcag aataacccgg caaaccgatg gtcggaatgc cgccgtctgg cgtgttgtgc
    19921 tgggtgacat gcagataatt aagatacaaa cgattcgctg taccaaggcc aaaagcgata
    19981 gaaggggcga cgccgtaacg ctcatttttg actttgtcgc gtccggcatc gtgcgttttt
    20041 tcgcccatca cattcaggcg caccgcagtg gtatcaccaa tgacctgatt gacgtccagc
    20101 gtgccgcggc ggaaccaggc gctgccaata ctggcggagg cgtcaatgcc ggaatcattg
    20161 cgcggctgct tgctgatcat attgatcgag cccgtcggcg cgctgcgccc gtagtcggtg
    20221 ccggaaggcc ctttaatcac ttcgacctgc tcagtattga aggtgtcgcg cgagacgctg
    20281 ccgatatcgc gaatgccatc aatataaata ctgttagagg tatcagcacc gcgcatataa
    20341 atggcgtcgc cagtggtgga gttaccgttc tcacccgcaa aaaacgcacc cacgccgggg
    20401 acgtttttga gcgcgtcggt aaggttggtt gcgccctgat ctttaatcac ttgttcagaa
    20461 atcaccgtca tcgtgcgggt agtatccgct accggacgcg agaatttcgg atcggcagat
    20521 tgttgtggcg cataaagcga aggcgttgat gcttcgacaa ccagcgtgtc atccgcgtta
    20581 gtttgccctt cggcggcgag tgcctgagcc acaggcgtga tgccaataca aagaccggca
    20641 aagaacgtga gcgaatgaaa ttgtctggca gggaaattgc gattgttttc cattttgcag
    20701 gtgacttttt cttatatgtt gaaattttca ctgccacgag aaagcatctc tgcggatgcg
    20761 atgccactgt taatcccgtg atagtgttcg tgcaagtgat aataattttg ataagcatta
    20821 tcacttggcg cgattttcta tcacccattg cgcgaaaaag aaatacattt atttacattg
    20881 agttcatatt tgatgttttt tgattacaaa acaggcgaaa actgcatcca gaggcgaatg
    20941 cagttacgtt ggtggggtta tttgtagatg gttgcagtac cgaacatctg gtcatttccg
    21001 cctgcggaat taatgacata acctttcgcg ccttgctcgc gcgctttttc tgccagttta
    21061 tcctccagat cgcttaggtt tgatgcgcca gttgccgaaa cggtaccggc gggccgtagc
    21121 tgaccgctgt ccaggttact catcggttgc gcggcataag ccgtgagaaa gattccgctc
    21181 aggacggtgg caatcagtag tgtgaggcac tttttcataa tgacatcctc ataaatacaa
    21241 cgggtagtgc ctgataagtg taggtcgcct gggcgatgaa aatgacgcaa aaaattgaca
    21301 gagat
//
LOCUS       DAPGWD010000065        20855 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPGWD010000065 DAPGWD010000000
VERSION     DAPGWD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.065"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(156..902)
                     /gene="cutC"
                     /locus_tag="RHX88_002428"
     CDS             complement(156..902)
                     /gene="cutC"
                     /locus_tag="RHX88_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDV2231105.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFLGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(916..1482)
                     /locus_tag="RHX88_002429"
     CDS             complement(916..1482)
                     /locus_tag="RHX88_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV2231106.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1698..3431
                     /gene="argS"
                     /locus_tag="RHX88_002430"
     CDS             1698..3431
                     /gene="argS"
                     /locus_tag="RHX88_002430"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDV2231107.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3608..4096
                     /locus_tag="RHX88_002431"
     CDS             3608..4096
                     /locus_tag="RHX88_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDV2231108.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSDDDS
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4214..4911)
                     /locus_tag="RHX88_002432"
                     /pseudo
     CDS             complement(4214..4911)
                     /locus_tag="RHX88_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4992..5384)
                     /gene="flhe"
                     /locus_tag="RHX88_002433"
     CDS             complement(4992..5384)
                     /gene="flhe"
                     /locus_tag="RHX88_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDV2231109.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5384..7462)
                     /gene="flhA"
                     /locus_tag="RHX88_002434"
     CDS             complement(5384..7462)
                     /gene="flhA"
                     /locus_tag="RHX88_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDV2231110.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GETTVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTAIIGGK"
     gene            complement(7455..8603)
                     /gene="flhB"
                     /locus_tag="RHX88_002435"
     CDS             complement(7455..8603)
                     /gene="flhB"
                     /locus_tag="RHX88_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDV2231111.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8805..9449)
                     /gene="cheZ"
                     /locus_tag="RHX88_002436"
     CDS             complement(8805..9449)
                     /gene="cheZ"
                     /locus_tag="RHX88_002436"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDV2231112.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(9460..9849)
                     /gene="cheY"
                     /locus_tag="RHX88_002437"
     CDS             complement(9460..9849)
                     /gene="cheY"
                     /locus_tag="RHX88_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDV2231113.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9864..10913)
                     /gene="cheB"
                     /locus_tag="RHX88_002438"
     CDS             complement(9864..10913)
                     /gene="cheB"
                     /locus_tag="RHX88_002438"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDV2231114.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10916..11776)
                     /gene="cheR"
                     /locus_tag="RHX88_002439"
     CDS             complement(10916..11776)
                     /gene="cheR"
                     /locus_tag="RHX88_002439"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDV2231115.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTKVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11795..13396)
                     /gene="tap"
                     /locus_tag="RHX88_002440"
     CDS             complement(11795..13396)
                     /gene="tap"
                     /locus_tag="RHX88_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDV2231116.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEYEVARHESVQLQIAPVVS"
     gene            complement(13442..15103)
                     /gene="tar"
                     /locus_tag="RHX88_002441"
     CDS             complement(13442..15103)
                     /gene="tar"
                     /locus_tag="RHX88_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDV2231117.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVIHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(15248..15751)
                     /gene="cheW"
                     /locus_tag="RHX88_002442"
     CDS             complement(15248..15751)
                     /gene="cheW"
                     /locus_tag="RHX88_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDV2231118.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15772..17736)
                     /gene="cheA"
                     /locus_tag="RHX88_002443"
     CDS             complement(15772..17736)
                     /gene="cheA"
                     /locus_tag="RHX88_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDV2231119.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17741..18667)
                     /gene="motB"
                     /locus_tag="RHX88_002444"
     CDS             complement(17741..18667)
                     /gene="motB"
                     /locus_tag="RHX88_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDV2231120.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(18664..19551)
                     /gene="motA"
                     /locus_tag="RHX88_002445"
     CDS             complement(18664..19551)
                     /gene="motA"
                     /locus_tag="RHX88_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV2231121.1"
                     /translation="MLILLGYMVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(19678..20256)
                     /gene="flhC"
                     /locus_tag="RHX88_002446"
     CDS             complement(19678..20256)
                     /gene="flhC"
                     /locus_tag="RHX88_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDV2231122.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(20259..20609)
                     /gene="flhD"
                     /locus_tag="RHX88_002447"
     CDS             complement(20259..20609)
                     /gene="flhD"
                     /locus_tag="RHX88_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDV2231123.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4964 a         5750 c         5114 g         5027 t
ORIGIN      
        1 ttagcacaac gctatgcgca ctgtcacccg ctcaacagat cacggttatc gttcgttttt
       61 tatactgctc agggataaaa aaaggcccct gttgaaattg caggggcctg gtacgagcaa
      121 gcatcatatt gggcgacatg atgcaacggt aaaaatcatt tggcctgatg gcgttcaatg
      181 attcctttca tttcagcaac cgccgccccg tctacgacat aacgcgaata ctcgtccgcg
      241 tgtgcatctg atgacataga caatccttga ttacgataac gcatcggtga ggcttgccac
      301 gctcccgcgg agctatggac ttccagcact ccggcatcga ggaagtggtg caggttttct
      361 gcacggactc ctgctccggc cataatgatt ggagcatcac gatgggcaat aagttccata
      421 atttttgata aaccttgcag tgcgtctgat ttttgccctg atgtcagcac ccgggcaatg
      481 cccaattccg caagattatt gagtgtattt agagggttag cgcacatatc aaaggcgcga
      541 tgaaaagtca ctgccagcgg accggcagca gccattattt tttccattcg tggcatatcg
      601 acattcccgt caacatcgag aacgcccgtc accagtccaa gaaaacctaa ttcgcggacc
      661 gtgcgcacat cctcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa atcaccaccg
      721 cgtgggcgaa ttatcggatg cacagggatc gtcacccgct ggcgcacgga tttcagtaca
      781 cccaacgacg gcgttaagcc cccctctttt ggggctgcgc ataattcaac tctgtctgcg
      841 ccgttttgct gcgccgttag tgcacattcc atgctgtaac agcaaatttc cagtaatgcc
      901 atttttactc cttaattacg ccgactgctc gctggcaacg atctcttcaa tggaccacgg
      961 atgaaactta atagttgttt tgccatcggt gaccgccagc gtcggattcg gtaatcgctc
     1021 atgctcacct tttggcgaac tggttttcac tgagatcccc ggtaggctga gtccttcatc
     1081 agaaagcaat gccagcgcac gggcattcag cgtttctgga tcacccggca gaacgatttc
     1141 aatatgttcc caaccttcgt gtgggtaacg tttttccccg ggccacggta gctccacaat
     1201 agaaaactgc cagtgcgcaa cctgtaccgg ttcatgcaat ttaaacagac aaatcggtct
     1261 gccattgatc atattttctg acaaaagctc gccacactgt tcaaacccgc gacgccagcg
     1321 ttcagcagtg gcgttttgat ggcaacgcaa agaaatgtga tcggcagtca gcggagtgat
     1381 attcaacccc agacggcggg aaagttcatc taatgcgtgg gtaaatcgcg gtaaatccga
     1441 tgcaatatcc tgcagctcgt cgatagattg ccagttcgcc ataatcactc ttcgtctttc
     1501 agtaaaagcg ttaatttacc ctgttgccct gtgccaacca accgctgatt tcacgccgct
     1561 tctgatgcaa tagtgaaaac ggcaatacgc cacgcgcacg ttgctgacga aaacagccat
     1621 ttgcagtata ctcccgccct aatttcttta actggtgcgg gcaatttttg ctcgcttcat
     1681 caatgtaagg tattccggtg aatattcagg ctcttctctc agaaaaagtc cgtcaggcca
     1741 tgattgcggc aggcgcgcct gcggattgcg aaccgcaggt tcgtcagtca gcaaaagttc
     1801 agttcggcga ctatcaggct aacggcatga tggcagttgc taaaaaactg ggtatggcac
     1861 cgcgacaatt agcagagcag gtgctgactc atctggatct taacggtatc gccagcaaag
     1921 ttgagatcgc cggtccaggc tttatcaaca ttttccttga tccggcattc ctggctgaac
     1981 atgttcagca ggcgctggcg tccgatcgtc tcggtgttgc tacgccagaa aaacagacca
     2041 ttgtggttga ctactctgcg ccaaacgtgg cgaaagagat gcacgtcggt cacctgcgct
     2101 ctaccattat tggtgacgca gcagtgcgta ctctggagtt cctcggtcac aaagtgattc
     2161 gcgcaaacca cgtcggcgac tggggcactc agttcggtat gctgattgca tggctggaaa
     2221 agcagcagca ggaaaacgcc ggtgaaatgg agctggctga ccttgaaggt ttctaccgcg
     2281 atgcgaaaaa gcattacgac gaagatgaag agtttgccga gcgtgcacgt aactacgtgg
     2341 taaaactgca aagcggtgac gaatatttcc gcgagatgtg gcgcaaactg gtcgacatca
     2401 ccatgacgca gaaccagatc acttacgatc gtctcaacgt gacgctgacc cgtgatgacg
     2461 tgatgggcga aagcctctac aacccaatgc tgccaggaat tgtggcggat ctcaaagcca
     2521 aaggtctggc agtagaaagc gaaggggcga ccgttgtatt ccttgatgag tttaaaaaca
     2581 aggaaggcga accgatgggc gtgatcattc agaagaaaga tggcggctat ctctacacca
     2641 ccactgatat cgcctgtgcg aaatatcgtt atgaaacact gcatgccgat cgcgtgctgt
     2701 attacatcga ctcccgtcag catcaacacc tgatgcaggc atgggcgatc gtccgtaaag
     2761 caggctatgt accggaatcc gtaccgctgg aacaccacat gttcggcatg atgctgggta
     2821 aagacggcaa accgttcaaa acccgcgcgg gtggtacagt gaaactggcc gatctgctgg
     2881 atgaagccct ggaacgtgca cgccgtctgg tggcagaaaa gaacccggat atgccagccg
     2941 acgagctgga aaaactggct aacgcggttg gtattggtgc ggtgaaatat gcggatctct
     3001 ccaaaaaccg caccacggac tacatcttcg actgggacaa catgctggcg tttgagggta
     3061 ataccgcgcc atacatgcag tatgcataca cgcgtgtatt gtccgtgttc cgtaaagcag
     3121 aaattgacga agagcaactg gctgcagctc cggtaatcat ccgtgaagat cgtgaagcgc
     3181 aactggcagc tcgcctgctg cagtttgaag aaaccctcac cgtggttgcc cgtgaaggta
     3241 cgccgcatgt gatgtgtgct tacctgtacg atctggccgg tctgttctct ggcttctacg
     3301 agcactgccc gatcctcagc gcagaaaacg aagaagtgcg taacagccgt ctgaaactgg
     3361 cacaactgac ggcgaagacg ctgaagctgg gtctggatac gctgggtatt gagactgtag
     3421 agcgtatgta atcgattttt cgtgagagtg aagcctgatc agggttacct gatcaggctt
     3481 ttttattgcc atctaaatgt attctgaaaa tggacatgcc atcgttttct cactgttggg
     3541 taagaagtca gaagcgtaat atccggcccc agggaaacga taacggttga atttaaggaa
     3601 taccgcagtg tttaaatttc ttgtattaac attaggcatt atctcttgcc aggtttacgc
     3661 agaagatacg cttatagtaa acgaccatga catttcagcc atcaaagatt gttggcaaaa
     3721 aaattcagat gatgatagtg acattaacgt gatcaaatca tgcctgcgac aagaatacaa
     3781 tctcgtcgat gcgcaattaa ataaagccta tggtgaagct tatcgttata tagaacaagt
     3841 gccacgcaca ggtgtaaaaa aacctgatac cgaacaactt aacttgctta aaaaatcaca
     3901 gcgagcctgg ctggatttta gggacaaaga atgtgaatta atcctttcaa atgaggacgt
     3961 tcaggattta agtgaccctt attctgaatc agaatggctc tcatgtatga tcatacagac
     4021 caatacgcga actcgccagt tgcagctata ccgtaactct gaagattttt atccaagccc
     4081 tttgacaaga ggataattca catatttttg gcatgttttg ttgcaagcta ttcctgataa
     4141 ataattgcaa caaaacatcg agcctttttc actgagttat taaacatact cgcgagcggg
     4201 taatgactcc aacttactga tagtgtttta tgttcagata atgcccgatg actttgtcat
     4261 gcagctccac cgattttgag aacgacagta acttccgtcc cagccttgcc agatgttgtc
     4321 tcagattcag attatgtcgc tcaatgcgct gagtgtaacg cttgctgata acgtgcagct
     4381 ttcccttcag gcgggattca tacagcggcc agccatccgt catccatacc acgacctcaa
     4441 aggccgacag caggctcaga agacgcgcca gtgtggccag agtgcgttca ccgaaaacgt
     4501 gcgccacaac tgtcctccgt atcctgtcat acgcgtaaaa cagccagcgc tgacgtgatt
     4561 tagcaccgac gtagccccac tgttcgtcca tttccgcgca gacaatcaca tcactgcccg
     4621 gttgtatgcg cgaggttacc gactgcggcc tgagtttttt aagtgacgta aaaccgtgtt
     4681 gaggccaacg cccataatgc gtgcactggc gcgacatcca acgccattca tggccatatc
     4741 aatgattttc tggtgcgtac cgggttgaga ggcggtgtaa gtgaactgta gttgccatgt
     4801 tttacggcaa tgagagcaga gatagcgctg atgtccggca gtgcttttgc cgttacgcac
     4861 tacgccttca gtagcggagc aggaaggaca tctgatggaa atggaagcca cgcaagcacc
     4921 ttaaaatcac catcatacac taaatcagta agttggcagc attacccgcg agcgcgtaat
     4981 ttttttgtcc ttcagcgata attcacaatc acttcattac gttgtacttt cagcggcgga
     5041 attaaccgcc caccgccagg cacttcccag ataaagcgca acggttctgc tgccgctata
     5101 ccggaaaagg ccacggtggt tccgctctgc ccctctaatt cgacacaacg agattgcgaa
     5161 cacaagcgaa cccgcagtcc tgaaggtgtc gggccgataa gctgataacg ccacgctacc
     5221 agcgtcatca atcctgaagc aggttgtcgc gtagaaagag gcgcagacga catcgactca
     5281 ccgcgatgat ttagcgtaat tcctacacta cttgcctgcc acatcccctc cccggcggct
     5341 tgcaccagca gcggaaacaa taatattgct aataaggttc tcattatttg ccgccaatta
     5401 tcgccgtcat gcggatatgt cggttatcag acagttccag attcgacagg accactaact
     5461 gcggcaagct gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca
     5521 atactggcgg cgcacccagc atctcctgac gggatagcgc ttcctgagtt tgcgccagta
     5581 aacgatccgc cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac
     5641 gttccagcgg tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct
     5701 gggtaatcgc ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt
     5761 ggatgggcgc atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt
     5821 tttcatcgag gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa
     5881 cgagatcttc cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct
     5941 cctgacgacc aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta
     6001 ccaccgtgct ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac
     6061 tttcaatcca gatagcattc aggccaaatg ccggatcgac ggtcgtctca ccaggtaacg
     6121 tcccggcagc ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc
     6181 caatctccac gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc
     6241 gaatgtgcac cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc
     6301 gtatacggcc caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac
     6361 ccacttccat tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg
     6421 tattattctc tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc
     6481 gtatccacca ggccagcccg agcaatccgg cagtgaacag caaaaatacc aggttcggca
     6541 ttccaggcac caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt
     6601 tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa
     6661 cgatgacccc cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc
     6721 caatggtcaa tagcgtataa ctttccgccg cgtgtcccat gctcatgcca tgttgcagca
     6781 cgccgaccag caacccgccg acaacgttaa tgaccatgat gaggatcccg gcgatggcat
     6841 cgccgcgaac aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca
     6901 cttcggagcg gcgttttttc gcctcatctt caccaatcaa tccggcgtta aggtcggcgt
     6961 caatcgccat ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcga
     7021 tacgcccggc acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca
     7081 cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accaccttcc
     7141 ctgccgccgc cgcgccggta tgcccttcca ttaaaatgat acgggttgaa gccacgttaa
     7201 gtgccagacg caacagcgtg gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg
     7261 tgcgctgggt aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa
     7321 acaacaggtc gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga
     7381 tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt caggtttgcg ggcaggcgca
     7441 gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc
     7501 accggaagat gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt
     7561 tgccagaccc aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga
     7621 ccaatctccg catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg
     7681 ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc
     7741 actttcggtg cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg
     7801 ttattgacaa tgacatccgc tttcggcaca tcggccatca tccgacgccg tgcggcagct
     7861 cgctgcatct gacggatccg tcctttaaca tgggggtcgc cttcgctttg tttgaactca
     7921 tcacgaatat cctgccgcga cattcgcagc tttttcaggt ggctgaagat ttggaaaaag
     7981 acgtcaaatc ccaccattgg aatgacacca agcaccacca gcagtgcgca tagccctacc
     8041 aaatccatcg cattacccat ggcggtaatc ggagactcgg ccattaagcg catcatctgc
     8101 ggccagtgat gccagagaaa aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt
     8161 gctttaagca actccgcgcc agtctgagcc gagaacatcc gtttaatgcc cggtagcggg
     8221 ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc tcccagcatg
     8281 accggagaaa tgagcgccac cagtaccacg ccgctaatca gtggcagcag cgccagcatg
     8341 gcttctctga tcagcagaat aatctgcccg aggatcagat tcgggtcttt gataatactg
     8401 tgatcaaaat gcagcccagc ggagagcatg cccgacaatc gacgggccag cgacacaccg
     8461 ccaaaccaga taacactaac gcccaccagc aaaatcagca gtgaggtcag ttcacgggaa
     8521 cgcgggattt gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg ggcttctgtt
     8581 ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa gagtattaag cgtgaatgat
     8641 gccagagcgc aaagcgttca atggtttgag taaggggcaa aacaggcggg atttagggct
     8701 tttgctgcca cacatcaagc atagtgtgcg tttgtcggat gcggcgtcat cgccttatca
     8761 gaccgcctga tatgacgtgg tcacgccaca tcaggcaata caaatcaaaa tccaagacta
     8821 tccaacaaat cgtccacctg atcctgactg gctaccacac cggctttgct ggtatcgacc
     8881 tgaggtccat taagcaaact ctggttttca cgttttggac gcgactcctg ttccgggatg
     8941 ttttccaaca gcaccatcag caactggcgt tcgatctcct gaatgacatc catcatccgc
     9001 ttaatgacct gcccggtgag atcctgaaaa tcctgcgcca tcatgatttc cagcagttgc
     9061 gcgttagtaa agctggtatg cgcgggtaca tctgccagaa attgtcgtgt atctgttacc
     9121 agttcacggg cgtcggcaag gtcaatcgga tcggcaaacc agtcatccca acgttgggtt
     9181 aacgcttttg ctgatttctc catttgatcc tgatgcggtt gtgacgcctc aacactgttc
     9241 agcgcccgct ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg atcgcgcgca
     9301 tcggggatgg cttccgccgc ttcggcaatg gcctgatcca gccccagttc ccgcaaactg
     9361 tcgcgcagca tacgcgtcag gctgccgatg cgcgcaatga tatcgccagc tgaatgctcg
     9421 tcagcaggtt tgattgatgg ttgcatcata gtcgcatcct cacatgccca gtttctcaaa
     9481 gattttgttg agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca catagccact
     9541 ggcccccgct tgcgccgcag caatgatgtt ctctttcttc gcttctgcag tcaccattaa
     9601 cactggcaat gccgacatcg cgccatccgc acgaattgtt ttcagcaatt ccaggccatc
     9661 catattgggc atgttccagt cggagataac aaatccataa ccgcctgcct gcaacttatt
     9721 gagagcgtcg acgccatctt ccgcttcctc aacattattg aatcccagct ctttcagcag
     9781 gttacgcact atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt taagttcttt
     9841 atccgccatt tcacactcct gatttaaata cgtatcgcct gtccggcact aatttttgcc
     9901 aacatttgct ggcttacctg gctaagatcg accacttcgc agacaccacc catattgatg
     9961 gcctcgcgcg gcatgccgaa caccacgcaa cttgcttcgt tttgcgcaag ggtccatgcc
    10021 cccgcctgac gcatcgccaa cattcctgcc gcgccgtcgt tgcccatacc ggtcaggatc
    10081 accccaaccg cattacgccc cgcctgtttg gcgacagaat ggaacaacac atctaccgaa
    10141 ggccgatgac ggttaaccgc cgggccatca tgaattttga tttggtaatt tgcgccacta
    10201 cgcgccagct ccatatgccg atcgcccggc gcgatatagg catgccccgg caaaacacgt
    10261 tctccgtctt cggcttcttt aaccccgatc tgacaaagct tattaagtct gtcggcaaaa
    10321 gagcgggtga aaccaggcgg catatgctgg gtaattaaca gtgccgggct ggaaagcggc
    10381 aacggttgca gtacgtgacg aattgcctca gttccacccg ttgaagcacc aatcgcaatc
    10441 agtttttcag aactcaacaa cggccctgcc ttcagcgttg tcggtgccga caatggctta
    10501 tgtgctgcaa ggctcgcctt tgctgctgta cgcacctttt cagcaatcat ttcgctatac
    10561 gccagcattc cttcgcgaat acccagttgc ggtttggtga caaaatctat cgcccccagc
    10621 tccagcgcgc gcagcgtgac ttctgaccct ttgccggtca gggaagaaac catcactacg
    10681 ggcattggac gcaaacgcat taatttttcg aggaaatcca gtccgtccat ccgcggcatt
    10741 tcaacatcca gcgtcagcac atcgggattg aatttcttaa tcaagtcacg cgcgaccagc
    10801 ggatcaggcg cggtcgccac catttccatg tcgctatggc tgttgatgat ttctgtcatg
    10861 atctggcgca tcagtgccga atcatcgaca gataacaccc tgattttgct catcgttaat
    10921 ccttacttag cgcatacacc gtctgaccac gcagcgtgaa gcggcgctca aggtggctaa
    10981 agttttcaga gtgacccgca aacagcaatc cgtcgggttt aaggagcgga acaaagcggc
    11041 gcaaaatctc ctgctgggta ttttgatcga agtagatcat gacgttacga cagaagatcg
    11101 catcaaacgg ccccggcacg gtgtactgtt tcgccagtag attcagcggg gcaaaatcaa
    11161 catagttcgc cagctcctga cgcacgcgta ccagcccttc atgcggtccc gtccctcgca
    11221 tgaaataccg ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg cgataaatac
    11281 cgcttctggc tttttccagc actttggtgt cgatatcact ggcaaacact ttccagcgtc
    11341 cgggcgcggt gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg
    11401 tcgaagccgc cgcgctccat acgcgatact cgccagaacg gcgacgtgcg tgatccgcga
    11461 gcagagggaa atggtgtgcc tcacggaaaa atgccgtcag attcgtggtc agcgaattga
    11521 taaacgcctg ccactcaccg ctgtgctgat tagattccag caagttcaga taatgaccga
    11581 aatccgtcag tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt
    11641 tatggtcagc cagaacgatc ccggctcgtt gatagatcaa ttgacttatc cgccgaaaat
    11701 gcgcgtcgga aagcgccagg cgctcggtca tctgtaacaa taaagacgtt tgcccacagg
    11761 gcagagatga agtcatagcg ccttctcaat cacttcagga taccactggc gcaatttgta
    11821 actgcaccga ctcatgtcgt gctacttcat attcttcaag ggtaaatacc gccacacgcg
    11881 acgaaagatg gtcggcctga ttcgccagtt gttccgttgc caccgccgct tcttctacca
    11941 gcgaggcgtt ctgctgagtc acctgatcca tctggctgac agcctgtgca acctgctcaa
    12001 tcccccgctg ttgttcttcc gacgcagagg caatttctcc cataatgtcg ttcacgcggg
    12061 tcaccgaact gacaatatcg atcatggtcg cggcggcgtt attcaccagt ttcgaaccct
    12121 gctggacacg attgactgac tcttcgatca gccctttgat ctcttttgct gcctgcgcgc
    12181 tacggctggc aagattgcgc acttcacctg ccactaccgc aaaaccacgc ccctgctctc
    12241 cggcgcgagc cgcttccact gccgcattca gggccagaat attggtctgg aaagcaattc
    12301 cgtcgataac gctgataatg tcgccaattt tttgcgagct ggtggcgatc tcctgcatgg
    12361 tgtgagtcat ggtactgacc tgaacaccgc ccgcctgcgc cgttgtcgcg gcattttttg
    12421 ccagttccga cgcctgtcgt gcgttatcgg cgttttgccc taccgtggcg gttaattgct
    12481 ccatactggc ggccgtttgt gccagcgatg ccgcctgctg ttcggtacga cttgagagat
    12541 cgttattgcc tgcgacaatc tccgcgatac caatgtgcat ctcctggctt cccttacgca
    12601 catcacttac cgtcccacgc aaagcctgct gcatggtctt cagactggca aaaatggcgg
    12661 tgatctcatt acgaccatat accgcaatcg gacgcgccag attacccgca gcaatgctgt
    12721 caaaatggct accgataatg gccagtggtt gaacaatcat cttgcgcgtc caccacagcg
    12781 cactgctgat gtagatcgcc gcaacaataa tcatgctgat aaacaccagt gccgaaatct
    12841 gatagttacg ctggctttgc gcactggcgg cttccagcac atggttgatc tccagctgcc
    12901 aggcctcaaa gttaacgtca aacgcattct gtgatccctg caccggcgca gtgaggaaat
    12961 ccgaaagttg attactttca agccaggtcg cctgatgttc gagatcgttg tgccagcggg
    13021 caaaactttt ctccgtctct ttttgcaatg ccctgacgtg ctcgttgccc gcagtcatcg
    13081 ccataaaact tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc gtcgtcatca
    13141 acgttttaat gtcatccgcc ggatagctaa gtgccgtcag agtgcccgct ttgttcagcg
    13201 cggtactggc ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt tgctgattgc
    13261 tctgctcgac ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa gacatgccgt
    13321 tactgccaat ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc
    13381 gaatacgatt aaacatcaac gctccccatc aggcggcaat gaccgcgtta gtaaatactc
    13441 gtcaaaatgt ttcccagttt ggatcttgtt cagcaattcg cagtcgtggc tgtgccggtg
    13501 gttgctcact ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg ctggctgcca
    13561 gacggaacgc ggaaaccgct tgcgttaaac gactcgcctg ttcttccagc gcagcggcgg
    13621 cggcagctga ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc atttccgaaa
    13681 ccgccaatgc gacttgatcg atgccacggc tctgttcatc cgatgccgat gcaatctcgc
    13741 ccataatgtc agtcacgcga gtgacagcat tgacgatatt gttcattgtt tccccggcgc
    13801 tttcgaccag caccgaaccg gtatcaacgc gtgagacgga gtcttcaatg agggctttga
    13861 tctcttttgc cgcctgggcg ctgcgactgg caagattacg cacttcaccc gccaccacgg
    13921 caaaaccacg gccctgttca cccgcacgcg cggcttcaac cgcggcattc agcgcgagga
    13981 tattagtctg gaaggcaata ccgtcgataa cgctgataat gtcggcaatt ttcttcgaac
    14041 tatcggcgat ctcatgcatc gttttcacta cgccatccac cactttgccg ccgtgctggg
    14101 cggtgtcgga ggcactttgc gccagttgcg aggcctggcg ggcgttatcg gcgttttgct
    14161 tcactgtcgc ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg gatgcctgct
    14221 gttcagtacg ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta ccggcataga
    14281 tggcatctga accttcgcgg acatgaatga cggtgtcagt caaagagcgt tgcatatgtg
    14341 aaacgctctg cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg gtattcgcca
    14401 ggttaccacc ggcgatttcg cgaatgtgag caataatttt tgccagcgga gtaagcaaca
    14461 tacggcgaat gccgtaccac gccaccagca gaatcaatac caccaccagc gcgataaccg
    14521 ccagttgcca ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata tcgcgataca
    14581 gtttttcact gctgagggcg tactgagcaa acgcttcgcc cattgcattt tgcattccct
    14641 gggttggctg agcgaaataa gctccagtat tgccataatc tagataatca atcagttcag
    14701 ttaacgctgt gtaatagttt ttatattttt catcaatatt acgactggta gcgaccattt
    14761 caggtaacgg tgccatgctt ttgaattttt tataatgcgt cgctgcctgc gccaatgttt
    14821 tcctggcgct atcgagcaat tcaactttgg cgttactttg ttgattggag gaatccatca
    14881 tcatccgtac cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg
    14941 acgtcagctc gccctgctgt tcccgtaatt gattggaaac cacaaagctc ttctggctat
    15001 ggtgaaggga agaaaaaaac agactgccgg aaataagctg taacagtgcg aataccccca
    15061 gcaccattac caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa
    15121 acaagttgat ctcgttatcg gcaaggaggg gggaaacttt attgctgatg ccacccgccg
    15181 cgaaattgaa ataaaaaacc cgatgcgcag atcatcgggt tcatttcaat tgaggaaatc
    15241 gggagaatta cgccacttct gacgccgcgc tatctaacag cgccatctct tcgctgttca
    15301 gcagtttttc gatgttcacc agaatcaaca tccggtcgcc cagtgcgccc agtccagtga
    15361 gatattctgt tgaaagcgtc acggcaaatt ccggtgccgg acgaatttgc tccgccgtca
    15421 atgaaagcac gtctgagacg ccgtcaacca cgatgccgac cacccgctgt ccgagattca
    15481 ggacgataac taccgtgttg tcgttatagt ccacatccac ctggctgaac ttaattcgta
    15541 agtcaacaat cggcacaata acgccgcgca gattcgtgac gcctttgata aacgctggcg
    15601 tgttcgcaat ccgtgttacc tgatcgtagc cacggatctc ctgcactttc aggatatcaa
    15661 taccgtactc ttcatcacca agggtaaata ccagaaattc ctggcctgac ggctcgctgg
    15721 ccagctttgt tacattcgtc ataccggtca tattgttacc tttttactca ttcaggcggc
    15781 ggtgttcgcc atacgttgtt cgcggtttat cgcctgcaag gcggagacat caacaatcag
    15841 tgccacgctg ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt tgcgatagtt
    15901 actttcaagg tttttaacca caacctggtg ttgaccaatt aattgatcca ccagcaaggc
    15961 gtagcggcga ccgccacttt gtaagatcac cacaattccc tgggtggctt cggttttcgc
    16021 gcccgcgacg ttgaacactt tccacagttc gacgatgggc agatattcac cccgcacttc
    16081 cagcacccgc tcgccgccgg ccagtggatg gagatcggct tcacggggtt gcagtgattc
    16141 cataacagca ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc
    16201 gaggatggcc agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac cctgcttcga
    16261 ctggatttcg acatgaccgc ccatctcctg gatattacgt ttaacgacgt ccatgccgac
    16321 gccgcgcccg gagacgtcgg tgacctgctc tgccgtggag aagccaggtg caaatatcag
    16381 catcgcgact tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg aggccgcttt
    16441 tgccagaatt cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt
    16501 gccgccctga tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag
    16561 ccgtttttct ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg
    16621 gtcgataatg cgttctatca ggcttttgtc gagttcagta gaactgccca ccagcgtcag
    16681 ttctacctgc ttgccgagtt ttcccgccag atcacgcacc agccggggat agcgactaaa
    16741 aacatattcc atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt
    16801 acgttgtaac tgccccatgc tggttatcaa atcaccatga ttaaccgggt ccagttcgct
    16861 ggaacgctgg gcaagcatgg actgggtgat aaccagctcg ccgacgaggt taattaattg
    16921 atcaaccttt tctaccgcta cacggatgct ggtggattca ttgctgcgcg tcgttttttc
    16981 ccgctccacg cggccggttg gcgcttgttc ggctgccagt ttaagcactg gtggggtgga
    17041 tatttttggc gagacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca
    17101 gagtaccgct gtgatgtcat cttcggcgat gtcgcccggt aatattgccg agagcgaatc
    17161 cgcccctttc accacgtcag ttaacgttgt cagatgtccc agttcttctt ccagcaggtc
    17221 gacttccccg gccttcaggc gcgaaaggat aattcgtcgc ggcgactgac tgcgactctg
    17281 ctcatcttgc ggttcacttt tggcaaccac acttaatcgg gtcactgcgg atggcgtttc
    17341 gcctttcgct tctaatgcca gttgacgcaa ggcctggcag atataatcga agctggcggc
    17401 atccggctct tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc
    17461 caaaaacaga ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcatcgag
    17521 caggttttcc atcagatgcg tggtttcctg caaaacgctg aagccaaaag ttcctgcccc
    17581 tcctttgatc gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc
    17641 cggctgcaaa accagcaaat gctgctccat gtcagccaac agttcgtccg cttcatcaaa
    17701 aaatgtctga taaaaatcgc ttatatccat gctcacgctg tcacctcggt tcggctgatg
    17761 gcattgtggg aacactgacc tgtggtgcaa cctcaggttt ttccagggcg cttactggct
    17821 cattctggct ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca
    17881 gcaggctgat gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg
    17941 ccgccatgcc gacgacacgt aacactttgc cgctatccaa ccctccgacc atcagttcgc
    18001 ggcgggatgc attggcccga tcggcagaaa gctcccagtt gctatatcct ttctcaccgc
    18061 tggcgtaggg gaaatcatcg gtatgacctg aaaggctaat acggttggga ataccgttca
    18121 gtacaggcgc aatggcgcgc agaatgtcgc gcatataggg ttcgacatcg gcactgccgg
    18181 ttctaaacat cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca
    18241 gatcgatttt gagatgggga cgtaacgccc gcagtttcgg atcggactct atcaactggt
    18301 cgagatcacc ccgcaatttc cgcaatcgac tttgctccat gcgttttttc agctcttcga
    18361 tgttcggctg cttattcact tccccctggc tttgggtgta atcatcacca ccgccgggaa
    18421 ttgggctttc actattagaa atgcgatcgc cgcccgtaac cgcagtcgcc agtggagtcc
    18481 ggaagtactc cgcaatctga atcagctctt ttgggctgga gatggagatc agccacatca
    18541 ccagaaaaaa ggccatcatc gcagtcataa agtcggcata agcaatcttc cacgatccat
    18601 gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat
    18661 tcttcatgct tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc
    18721 ttccagttca ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac
    18781 ggcgataggc ggtgcgtaac cgttcagatt agaaagcaga gtgactttga cgcactgcat
    18841 cattttgctg gtttcggcgc ttttctgacg taaaacagtc gctaatgggg aaataaatcc
    18901 gtaagccaat aaaatgccga ggaaagtccc caccatcgca tgtgcgataa gcgcacccag
    18961 ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg acccccatta cagccgcaac
    19021 aataccaaac gccggaagtg agtccccgac cagcgccaga ctgttcgccg ggacttctgc
    19081 ctcgctttcg tgcgtctcaa tctcttcatc catcagggct tcgatttcga aggtgttcat
    19141 gtgaccgctg ataatcaggc gcagataatc gacgataaaa tcaagcatga cgctatccgc
    19201 gaggatgcgt gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc
    19261 cagcgaaaac atccccatct gccgcgattt tgccatcaac cgataaagca gagccagcag
    19321 atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgccttcag
    19381 cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc
    19441 aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata
    19501 accgccgaaa actgtaccga gaacaaccat gtaacctaat aagataagca cgacatcatc
    19561 cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggca
    19621 aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt gcgcctcacc gtatcagtta
    19681 aacagcctgt actctctgtt catccagcag ttgtgggata atatcggcag gattctggga
    19741 aagtttacgt ctttttactg ccctggatgg cggttgacat aagctgcagg caaagctgcc
    19801 aacaggctgg tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg
    19861 cagtaatcca ctttcaacaa accgcaccaa tgtccaggca cgagttaatg ccagcagtgg
    19921 tccttcttct gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc
    19981 gacgccatta cacaaaccgg ttttcagtaa aaactgccat gcattacaga acatcgaagc
    20041 atgaacgttt tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg
    20101 cggtgggctt ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt
    20161 ttcgctttcc agcatctgca aacgagcgcc cagggtgatc aattccattg ccagctgaat
    20221 atcccgcgct tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc
    20281 gcttcttcag gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga
    20341 atttgctgga gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg
    20401 tcaaaacgga agtgacaaac cagctgattg gtttctgcca gcttaaccat ttgcggaaga
    20461 gtcagtgccg ctaatgttgt cgccatttct tcatttatgc cgagacgaaa catagcggac
    20521 gctttgtcct gaacaatcag acgctgtgca agtagtaaat atgacaagtt gatgtcataa
    20581 atgtgtttca gcaactcgga ggtatgcatt attcccaccc agaataacca actttatttt
    20641 tatgcggttt caccgcaccc tgtgatgtcg ccgggaagcc ccggtaaaaa taattagcat
    20701 tagaatagtt gcgataagct gcaataagca gagccacctt tttggtttaa taagtcctta
    20761 caaatagaag ggggtcttta cacttatcta agatttttcc taaatcgacg caactgtact
    20821 cgtcactaca cgcacataca acggaggggg gctgc
//
LOCUS       DAPGWD010000066        20358 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPGWD010000066 DAPGWD010000000
VERSION     DAPGWD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.066"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            25..840
                     /locus_tag="RHX88_002448"
     CDS             25..840
                     /locus_tag="RHX88_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2231124.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            842..982
                     /locus_tag="RHX88_002449"
                     /pseudo
     CDS             842..982
                     /locus_tag="RHX88_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            1084..1557
                     /locus_tag="RHX88_002450"
     CDS             1084..1557
                     /locus_tag="RHX88_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV2231125.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAVKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(1708..2961)
                     /gene="avtA"
                     /locus_tag="RHX88_002451"
     CDS             complement(1708..2961)
                     /gene="avtA"
                     /locus_tag="RHX88_002451"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDV2231126.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPDKIEAGVKILAEEIERAWAESH"
     gene            complement(3139..5169)
                     /gene="malS"
                     /locus_tag="RHX88_002452"
     CDS             complement(3139..5169)
                     /gene="malS"
                     /locus_tag="RHX88_002452"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV2231127.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSKADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVEFPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            5489..6313
                     /locus_tag="RHX88_002453"
     CDS             5489..6313
                     /locus_tag="RHX88_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDV2231128.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(6421..7599)
                     /gene="xylR"
                     /locus_tag="RHX88_002454"
     CDS             complement(6421..7599)
                     /gene="xylR"
                     /locus_tag="RHX88_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDV2231129.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(7677..8858)
                     /gene="xylH"
                     /locus_tag="RHX88_002455"
     CDS             complement(7677..8858)
                     /gene="xylH"
                     /locus_tag="RHX88_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDV2231130.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(8836..10377)
                     /gene="xylG"
                     /locus_tag="RHX88_002456"
     CDS             complement(8836..10377)
                     /gene="xylG"
                     /locus_tag="RHX88_002456"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDV2231131.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDKPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(10455..11447)
                     /gene="xylF"
                     /locus_tag="RHX88_002457"
     CDS             complement(10455..11447)
                     /gene="xylF"
                     /locus_tag="RHX88_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2231132.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            11813..13135
                     /gene="xylA"
                     /locus_tag="RHX88_002458"
     CDS             11813..13135
                     /gene="xylA"
                     /locus_tag="RHX88_002458"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDV2231133.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            13207..14661
                     /gene="xylB"
                     /locus_tag="RHX88_002459"
     CDS             13207..14661
                     /gene="xylB"
                     /locus_tag="RHX88_002459"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDV2231134.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHQPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            14830..15171
                     /locus_tag="RHX88_002460"
     CDS             14830..15171
                     /locus_tag="RHX88_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231135.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            15217..15654
                     /locus_tag="RHX88_002461"
     CDS             15217..15654
                     /locus_tag="RHX88_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDV2231136.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(15696..16691)
                     /gene="wecH"
                     /locus_tag="RHX88_002462"
     CDS             complement(15696..16691)
                     /gene="wecH"
                     /locus_tag="RHX88_002462"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDV2231137.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            16866..17165
                     /locus_tag="RHX88_002463"
     CDS             16866..17165
                     /locus_tag="RHX88_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDV2231138.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            17260..18171
                     /gene="glyQ"
                     /locus_tag="RHX88_002464"
     CDS             17260..18171
                     /gene="glyQ"
                     /locus_tag="RHX88_002464"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDV2231139.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            18181..20250
                     /gene="glyS"
                     /locus_tag="RHX88_002465"
     CDS             18181..20250
                     /gene="glyS"
                     /locus_tag="RHX88_002465"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDV2231140.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEALSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         5062 a         4976 c         5203 g         5117 t
ORIGIN      
        1 gaataagcgg gaaattacat atcaatgctc gaattatcca tgacgttacc gattaaagtg
       61 caaaatggtg gattatttat ttcccgtggc gtagggaggc atcctgcaag gcggctggag
      121 tcatgggaaa ttatctttgt cgaaaaaggt cgattaacga ttcaggaaga agaacggata
      181 tttctggtcg atgcgggaga gagtttatta ctctggccga accgacaaca tgttggggtg
      241 gaggattttc ccgccgatct caaattttac tggctgcatt ttgaggttaa agcgccggac
      301 aacgaccctc gctggcttac tcatcttagc gtgccgcaac aaacgaaggt tgtcgatccg
      361 caggcgctgg tggccctgtt tcgccagttc ttaagcgaac aggaaaagca tcaacgcact
      421 ccggcgctgg agtttattgt gctgctcatt ttgcagcagc tcacccgtga cgcgcgtctt
      481 gatgaacagg ccgatggcgc aggcaccacc cttgcctgga aagcgcagaa actgatccgc
      541 acgcaatatc atcgcccgct ttctacggcg cttctggccc gcgagctgta ctgcaatgcc
      601 gattatcttg ggcgcgtcta ccggcgggtg tttcatctga cgttaacaga agcaatccat
      661 cgccagcggg tgcgcgaagc cgaaaagctg ttaattagcg aagcgcgatc gctcacggaa
      721 gtggcggcgt tgtgcgggtt taatgacgtt ggctatttcc ggcagatttt tcgcaaacac
      781 accggtttaa cgccagcagc gtggaagcgg cgttacagca aggaacatat caattcgtag
      841 tgccgtgtcg atttcgcttt cgacatcacg tctgaaacag gtgggggaga aaaatctcct
      901 gaaaccactg cgtgaaaccg cgcaggctat ttctaatgaa ctgggattta ctgtcaggga
      961 tgacctgggc gcgatcacat aacgctttgg acaagtgcca aaactttaac atttccttcg
     1021 ttggatcaaa gcagtaggga cgcgctctct ggcactctgc tgttttagtg caaaggagtg
     1081 atcatgaacc ggtttattat tgcggatgcg acgaaatgta tcggttgccg tacctgtgaa
     1141 gtggcttgcg cagtgtcgca tcaggagaat caggattgcg ctgcgttgtc accagacgag
     1201 tttatttccc gtattcgtgt cattaaagac cacagctgga ccacggcagt agcctgtcat
     1261 cagtgtgaag atgcaccgtg cgcgaatgtc tgccctgttg acgcgataag ccgcgaacat
     1321 gggcatattt tcgttgaaca aacacgttgc attggctgta aaagctgtat gctggcttgc
     1381 ccgtttggtg cgatggaggt cgtttcttcg cgcaaaaagg cgagggcggt taagtgcgat
     1441 ctgtgctggc atcgggagac gggaccggcc tgtgttgaag cctgcccgac aaaggcgttg
     1501 cagtgcatgg atgtcgagaa agtgcagcgg catcggctac ggcagcagcc tgtttgaaac
     1561 gtttttccgg cgtttttaac gcagggatag tgcgctttgt ttatgccgga tccggctaaa
     1621 acgccttatc cggcctacaa attcttccaa attcaatata ttgcagaaat catgtaggcc
     1681 tgataagcgt agcgcatcag gcaattttta gtgactttca gcccaggctc tttctatctc
     1741 ttctgccaga atcttcacac ccgcctcaat tttatccggc tccggcacgt agttcatgcg
     1801 catacattga tgtgtatgtg gccacggttt atccagtcct gggaagaagt tgtgccccgg
     1861 caccatcagc acgccgcgtg ctttcaggcg ctggtagagt tgttcggtcg taattggcaa
     1921 atccttaaac catagccaga ggaaaatggc tccttctggt ttatgaatca ggcagcgatc
     1981 ttccggtaaa tagcggcgaa tgatggcgat agtttcctga acacgctggt agtaaaacgg
     2041 tttgatgact gtttcagaca ggcgcagcag atcgttacgc ttaatcattt cacacatcat
     2101 cgccggacca ataccgccag gtgccaggct gataatgccg ttcatattgg tgatggcggt
     2161 gatgattttt tcattggcga tgataatgcc gcagcgggag ccaggtagac ccagcttgga
     2221 aagactcatg cacagcacga tattcggatt ccatagcggg cgcgcttcac tgaagatgat
     2281 acccgggaac gggacgccat aagcgttatc aatcaccagc ggaataccgt gttgattcgc
     2341 cagcgcgtca agcttcagca actcttcgtc agtaatcaca ttgcctgttg gattcgtcgg
     2401 ccgggagacg caaatcatcc cggtttcttc gccaatatgc agatgctcaa aatcgacgtg
     2461 gtatttaaac tggccttcag gcagcagttc aatattcgga cgcgcagaga caaacagatc
     2521 ttcttccagt ccggcgtcag cgtagccaat gtattccggt gccagcggga acagcacttt
     2581 tttgacccga ccatcggcac ggcgtccggc aaacaggtta aataagtaga aaaacgcgct
     2641 ctggctgccg tttgttagtg caatattctg tggttcgata tcccaaccca acttctcgcg
     2701 cagcattccg gcaagcagtg tgagtagctc cgttttcccc tgtggaccgt cgtaattgca
     2761 cagtgcatca gtcgctttgc cactttccag catgtcggtc agtagcgtct ggaagtagtc
     2821 ctgcatttcc gggatctgcg ccggattacc gccgccgagc ataatcgcgc caggcgtgcg
     2881 taaaccgtcg ttcagatctt ccatcagcag cgtaatgccg gagtggcggg taaatttgtc
     2941 accaaaaagg gagaatgtca tagcgggata tctgtcgaac tctgtagcaa ggaaggtaac
     3001 aataacgcta catttgctgg ggtgcaaatc ggtctgttga agagtgagtg gtgctttatc
     3061 ttgcaacgct gattagggct gacattttat cctggttggc ggcttcccga ggtactgacg
     3121 aacgggaagc cggaaaagtc actgttgccc agcccagatg accagcactt tatcgtcgcc
     3181 atgctcacga acaaagccgt agccctgctt cagcgaaagt gtcgtttgtt tgcccgcgcc
     3241 aattgcggga tggcgggcgc ggaactggct gattttctgc cagtgcgcga cgctggcggc
     3301 agatttaccg ctaacatcct gccagttcat atccgaacgt gtaccttgca gcggatcaga
     3361 acctgtagga ccgaacggac gcgaggattc atcaccataa aagatttgta ccgcgcctgg
     3421 cgctaatagt aataactctg ctgctttgtc gcccccttca cggaacaggc gggtatcatg
     3481 cgacgagagg tagctcaaca cgttgaaacc ctgcaatttt tccgccattt gctgccaggt
     3541 cgtatccatc tgcgccagac aatcgactgc tttcgccgcc tgctcctgat aatcgaaatt
     3601 gatcatcgca tcgaagccgt ggcgatagta gtcactttgc atcacgccgt ggccccaggc
     3661 ttcaccggtc atccagaaag gtttgtcatc taatgctttg tcggggttag cttttttcca
     3721 ttcgcgaagc gcggcgctgg cttcggtttt cagttgctgc caggcgggaa actcaacatg
     3781 tttggcggta tcgacccgaa aaccatcaat cccatagtcg cggacccact gacttaacca
     3841 gtgggttaag taatcgcgag gtgtaaagcc ttcgatgact ttagcgtgag tatccgtttt
     3901 gtttttatag aacaccggca gaccagaagc ggtcgttgat tcggttttga tatccggcaa
     3961 aaaggccagc gacatagtga gatcgtcaaa tccaggattg tcgtaatcgc cgatatccgt
     4021 tctgatccag ttttttcccc accatttatc ccagcctgtt ttgtcgctga aattaatgta
     4081 atcgttaaag ctatgccagg tttgcccggc ggcaggtttc cagtcactcc agcgttcacc
     4141 cagcgttttt ttcagctcgt caccagaaag atataacgcg ccaaactgat actcctgcat
     4201 atccgccagc gtggcatagc cagtgtggtt catcacgaca tcaaagagaa tacgaatacc
     4261 gcgctgatgc gcgctatcaa ccagcgtccg tagatcggct ttgctgccca tattggcatc
     4321 aagattcgtc cagtcctgtg tgtaataacc gtggtaggca taatgcggga aatcaccttt
     4381 tgtaccgccg ccgacccagc cgtgaatttg ctcaaatggg gcgcttatcc acaaagcatt
     4441 gacgcccagc tgctggagat aatccagttt gttggtcagg ccgcgtaaat cgccgccgtg
     4501 aaaagtgcca atttccgcca taccgtcttt atgacgtccg taactctggt cattactggg
     4561 atcgccgttt tcgaaacgat ctgtcagcac aaagtaaacc gtggcgttat gccagtcgaa
     4621 aggggcggat gtgtcagttt ctgcccgttc cagcaggagt aaaccgttgc tggtggcagc
     4681 gggttgtaac gttatttgac cgttcttcac tatcgcaatt tgctggctgt aataatcccg
     4741 tacggcggct ccttccggaa aagtggcgct gacatccagc gtgagcggta atccatccca
     4801 tttcgggcat acacggacct ggcttgctac cggttcggcg gcgttctgga tggaaatcat
     4861 caatgttggc gtaccggagc gggtgtctat ttgcagcgta tattcgccgt ccctgaacaa
     4921 tcgccattga ggcggcgtgt tgctacaagg ttgcagggaa agcatctgat tgagttttat
     4981 cgcatccgca ggctgccagc actgttggtc aaaatttagc gttagtggac gcgtaccttt
     5041 gggcaactgc gcgtggctga caaatgttcc cgttccctgt tcactaaagg cagggaaccc
     5101 cggagaagtc cagctggcgg caacggcgaa gccaggaagg agtgtcagaa aacaggcggc
     5161 gagtttcatg gcggatgagt ccttattgct gcgatttcag acagtttgcc agcgataagc
     5221 caaacaaaac tcatccctga gcggtaagtc cacaggatga gaagcaaggg tgagcgatct
     5281 cgcgcaaaaa acggctgaat tttgcgataa accccacatt ttctgcgatt tagcgccaat
     5341 ctgaatcgtt aacacgtgac atagtttcag atttggacta ttccttgggg tgctcttgac
     5401 agctattttt gatatgattt gagattcaac tctcaaattt gtgaaaaata taaggtgttg
     5461 gaatgattaa atccgaccag gagacctgat gattttgact cccatacgac gatatggggc
     5521 gatgattctt atgttactca ctctggtgtt ttcgagtgag gtgttagcga agacgcacac
     5581 aacaacagcg agtcaaaagt cccacttaac taaagctagt aataaacagg taagcagtaa
     5641 acaagagtat tctcgcaata gtgcaaagag tagttcactt cctgatttgc gaaaataccc
     5701 ttccggaaca ccaaggaaaa aggcgtttct ccggaccgta atgccttaca ttaccagcca
     5761 aaatgcagcc attactgcag aacgtaactg gctgatttca aaacagtatc agggccaatg
     5821 gtcacctgct gagcgtgcgc gtctgaaaga catcgccaaa cgctacaagg tgaagtggtc
     5881 tggtaatacg cgaaaaatcc cgtggaatac cttgcttgaa cgcgtagaca ttatccctac
     5941 cagtatggtg gcgacgatgg ctgcagcaga aagcggttgg ggaacgtcga agctggcgcg
     6001 caacaacaac aatcttttcg gcatgaaatg catgaaagga cgttgtacca atgcgccagg
     6061 taaagtgaaa gggtactcac agtttagttc tgtcaaagaa tcggtgagcg cctatgtcac
     6121 taacctgaat acgcacccgg cttactcttc gttccgtaaa tcgcgtgcac agctgcgtaa
     6181 agcggatcag gaagtgactg ccacagcgat gattcacaag ctgaagggct actcgaccaa
     6241 agggaagagt tataacaact acctgttcgc aatgtaccag gataaccaac ggttaatcgc
     6301 ggcgcatatg taattgtatt tcctcgagcc ttatccgact tgtcggtcgg ataaggcttt
     6361 tgatcgcatc aggtggttgt gctgagttac ctgatgcgac gccgacaatt ctcatcatcg
     6421 ctacaacatg acctcgctat ttacatcgcg atactctttt ggcgtcgtgt catatgcttt
     6481 tttaaaaaca gagtagaaat attgcagcga tggataaccg cacatttgcg atatctcatt
     6541 gatcgacaag gtggttgaaa tcagcagact gcgcgctttc tccagcttct cggcatgaat
     6601 catggcatgg atggtttcac ccacctcttc tttaaaacgc ttctcaagat tggagcgcga
     6661 gatcccgacc gcatcgagta cctgatccac tttaatccct ttacaggcgt gattacgaat
     6721 gtaatgcatg gcctgaataa cggcgggatc ggtcagcgaa cggtaatctg ttgagcgccg
     6781 ttcaatgacg cgaactggag ggaccaaaat ccgctgtagc ggcatttctt ctttatctaa
     6841 taatcgatgc aacagttttg ccgcctgata gcccatttgc cgcgcgccct gagcgaccga
     6901 agaaagggcg acacgcgaca gatagcgggt cagttcttcg ttatcgatgc caatcacgca
     6961 taatttttcc ggtacgggaa tgtgtagatg ttcacatact tgcagaatat gccgcgcccg
     7021 ggcgtcagta acggcaataa tcccggtttg cggtggcagc gtttgtagcc agtctgccag
     7081 ccgattttgc gcgtgttgcc agttctctgg tgcggtttct aacccctgat aaaccactcc
     7141 gcgatacttt tcttcggcga caagctgacg aaatgcatat tcgcgttcag tggcccaacg
     7201 tttgccgctt gattccggaa gaccataaaa agcaaagcgg ttaacgcctt tctcttttaa
     7261 atgcaaaaat gcgctttcaa ccagcgcata gttatcggtg gcaatgtaat gaacgggtgg
     7321 gtaactttct gcaaggtgat acgagccgcc aaccccaaca atggggacgt cgacatcagc
     7381 cagcgcttgc tcgatctgtt tgtcgtcgaa gtcggcaatg acgccatctc ctaaccagtc
     7441 cttgatttta tcaatgcggg cgcggaaatc ttcttcaatg aaaatatccc attccgattg
     7501 tgacgcctgt aaatattccc ctacgccttc tactacctgc cggtcatagg ctttattggc
     7561 attgaacagt aatgtgatgc ggtgacgttt agtaaacatg gttcttttcc tgctgaatca
     7621 tgcaaaaact caaaaccggt aatacgttac cggctttgag aaaattttta tcaaaatcaa
     7681 gaacggcgtt tggttgcgga gtccatccat actgccagca acagaatcgc acctttaacg
     7741 atatactgcc agaaggtcgg tacatccatc atactcatac cgttatccag tgaagccatg
     7801 atgaatgccc ccattaccgc tccagccacg ctcccgacgc cgcctgccag actggtgccg
     7861 ccaatcacgc atgctgcaat tgcgtccagc tcggcgatat tccccgcaga aggtgaacca
     7921 gcgccaagtc gagaactgag gattaatccg gcgatggcta ccattaatcc gttaatagcg
     7981 aacacggcaa gtttggtgcg ttcaacgtta atcccggaga gacgtgctgc ttccagattg
     8041 ccgccgatgg cataaatgcg tcgtccaaat gccgtccgcg ttgccataaa cattccgccg
     8101 agtaacagca acgtcagcag cagaacagga gtgggaacgc cacgataatc attcaacagc
     8161 cagattgcgc ctaatacgat gatagcggtt aaagcctggc gaccgactac tgcggtagag
     8221 gccggagact gcaaacccaa agcctgacgg cgcattcttc cgcgccattg ccaaccaaca
     8281 aaagccatta agccaagcgc gccaatgatg aagccggtgc tggcggggag atagctttgc
     8341 ccaatttgtg acatcgcggc gctggtgggg gatacagttg tgccgttggt gatgccaatg
     8401 agtatgccgc gaaatgccaa catgcccgcg agagtgacaa taaatgaagg gactttgcgg
     8461 tacgcgaccc accatccgtt ccaggcaccg agaagcagtc ccagaaccaa cgtcacaatg
     8521 atggtaagtg gcaaaggcca gcctagccag acgtcacaaa tcgccgcgac gccacctaat
     8581 agccccatca ttgagccgac ggaaaggtcg atttcagcag aaattatgac gaacaccatt
     8641 cctaccgcga ggatgccggt aatcgcggtc tggcgtaaca ggttggagac gttacgggcg
     8701 cttaagtagg caccatcggt ggtccaggta aagaacagca tgattgcgat gatagctgca
     8761 atcatcacga agacctgcaa attcagtgat ttcagcccgg agaagccacc ggatgtcggt
     8821 acggccaatt tcacttcaga cggattgctt ttcgacatga tgttcgctcc tcaatgcggc
     8881 ttccatcacc tgctcctgag tcaggttatg atttatcagg ttggctttta gtttcccttc
     8941 atgcatcact agtacacgat cgctaaggcc gagcacttca ggtaattcag aagagatgac
     9001 aataacggca ataccctgct ggacgagttg gttaattaat ttgtagatct cgtatttcgc
     9061 gccaatatcg atacccctgg tgggttcatc aagaatgaga atgcgcgggt taagtaacag
     9121 acagcgagcg aggatcgctt tttgctgatt gccgccgctc aaacgtccaa tagcaaggtc
     9181 gggggacgac gttttaactt tgagttgctg gattgattcc agaatacatt tttgctctgc
     9241 cgcgtcatca agctggctaa tgccaccggt aaatttattg agtgcggcga gggtaatatt
     9301 tttaccaacc gccattaccg gaacgatgcc gtcgcgcttt ctgtcttcgg ggaccatcgc
     9361 aatcccctgg gcgatggctt gctgacagtt acgaatatct acctgtttgc catcaatata
     9421 aatttttcct tcccattgtc cgggccacac accaaacagg cactgaatgg tctcggtacg
     9481 tccggcacca acgagtccgg caatacccag tatttcgcca cgtttcaggg aaaacgagac
     9541 atcattaact cgtttaatat gacgattaac cggatgccat gccgtcagat gttcaatacg
     9601 taatatttca tctccggtgg tatgtggttc attagggtaa agcgcggtta actctcgccc
     9661 gaccatcatg gtgataatat cgtcttcact cattccggca gcatcacgcg taccaatgtg
     9721 ctgtccgtcg cgaataacgc aaatcgtatc ggaaatcgct ttgacttcgt tgagtttgtg
     9781 cgaaatataa atacaggcga taccgtgctg ttgtagatcg cgaataatat ccagtaaaac
     9841 cgacgtttcc tgctcagtta atgaggctgt cggtttatcg agaattaaca agcgcacctg
     9901 tttattaagt gccttggcaa tttcaaccag ttgttgttgc ccaagcccta aatcgccaac
     9961 gcgggtatca ggtgaaatgg ataaactgac ctgtgcgagc agcttctgac agcgtagcgt
    10021 catcaggtca taatccataa tgccattgtg ggttatttcg ttacccagga agatattttc
    10081 cagcacggtc aattctttca ccagggccaa ttcctggtga atgatggcga tacctttgcg
    10141 ttcggtatcg cggatgtgac tcgcctgaat ctcttctccc gcaaaaataa tttcgccttc
    10201 gtaggagcca tggggataaa taccacacag cactttcatc agcgttgatt taccagaccc
    10261 attttcccca cagagtgaga cgatttcgcc agcattcaac cgcaagctga cgttatcaat
    10321 cgccttcaca ctgccgaagg ttttggtaat gttcttcatt tcaagtagat aaggcataac
    10381 gactccacct aagccaattc attcacgcgg catggagaga aatcacgccc ccgctccgcg
    10441 ctggggcgta acgcttacag ctcgctctct ttgtggaatc cgtctttaat taccgtatct
    10501 ttgatgttgt ttttattcac atcgatcggt gtcaggaggc gggaggggac atctttcagg
    10561 ccattattca gtgaggtatc tgcttttggc tcctgaccat tgcccaattc aacggcaatt
    10621 tctgcggcag tatttgccag caatgtaata ggtttatata ccgtcatagt ttgcgtacca
    10681 gcagcaatac gtttaatacc tgcgagatcc gcatcctggc cagaaattgc tactttccct
    10741 gataaacctt gcgcgcttaa tgcctgaatt gccccacctg cggtggcatc gtttgaggca
    10801 actacagcat caattttgtt attattggcg gttagcgcgt tttccataat tttcaatgcg
    10861 ttttccggta accagccatc aacccattgg tcaccaacga ctttaatttt tccggaatca
    10921 acgtaaggtt gtaacacttt catttgtccg gcgcggaaca gcttagcgtt gttatctacc
    10981 ggcgagccgc ccatcaggaa gtaattacct tgcggaacaa tatcgaccag ggcttttgcc
    11041 tgcagttcac cgactttttc gttatcgaaa gaaatataaa aatcgatatc cgcatcgtta
    11101 atcatacggt cgtaagctaa tactttaatg ccttcttgtt tggcttcttt tacaacgtta
    11161 cttaatacct gaccgttata cggaataatg acaagaacat cgacaccccg gtttatcatg
    11221 ttttcaatct gcgacatttg tgtttcttca ttgccatttg cagactgtac aaatactttc
    11281 gcgccgagag attctgcctt tttcacaaag atatctcgat ctttttgcca gcgttcaaga
    11341 cggagatcat caatcgccat acctattttg acttctttgg cgtgtgccgc aacgttggta
    11401 agcagaagtg aggtgcaaag ggtgagtaga atgttcttta ttttcatggt gtagggcctt
    11461 ctgtagttag aggacagctt taataagtaa caatcaccgc gataaacgta accaattttt
    11521 agcaactaaa caggggaaaa caattacaga tttttatctt tcgattacga tttttggttt
    11581 atttcttgat ttatgaccga gatcttactt ttgttgcgca attgtactta ttgcattttt
    11641 ctcttcgagg aattacccag tttcatcatt ccattttatt ttgcgagcga gcgcacactt
    11701 gtgaattatc tcaatagcag tgtgaaataa cataattgag caactgaaag ggagtgccca
    11761 atattacgac atcatccatc acccgcggca ttacctgatt atggagttca atatgcaagc
    11821 ctattttgac cagctcgatc gcgttcgtta tgaaggctca aaatcctcaa acccgttagc
    11881 attccgtcac tacaatcccg acgaactggt gttgggcaag cgtatggaag agcacttgcg
    11941 atttgccgcc tgctactggc acaccttctg ctggaacggg gcggatatgt ttggtgtggg
    12001 ggcgtttaat cgtccgtggc agcagcctgg tgaggcactg gcgttggcga agcgtaaagc
    12061 agatgtcgca tttgagtttt tccacaagtt acatgtgcca ttttattgct tccacgatgt
    12121 ggatgtttcc cctgagggcg cgtcgttaaa agagtacatc aataattttg cgcaaatggt
    12181 tgatgtcctg gcaggcaagc aagaagagag cggcgtgaag ctgctgtggg gaaccgccaa
    12241 ctgctttaca aaccctcgct acggcgcggg tgcggcgacg aacccagatc cagaagtctt
    12301 cagctgggcg gcaacgcaag ttgttacagc gatggaagca acccataaat tgggtggtga
    12361 aaactatgtc ctgtggggcg gtcgtgaagg ttacgaaacg ctgttaaata ccgacttgcg
    12421 tcaggagcgt gaacaactgg gccgctttat gcaaatggtg gttgagcata aacataaaat
    12481 cggcttccag ggcacgttgc ttatcgaacc gaaaccgcaa gaaccgacta aacatcaata
    12541 tgattacgat gctgcgacag tctatggctt cctgaaacag tttggtctgg aaaaagagat
    12601 taaactgaac attgaagcta accacgcgac gctggcaggt cactctttcc atcatgaaat
    12661 agccaccgcc attgcgcttg gcctgttcgg ttctgtcgac gccaaccgtg gcgatgcgca
    12721 actgggctgg gacaccgacc agttcccgaa cagtgtggaa gagaatgcgc tggtgatgta
    12781 tgaaattctc aaagcaggcg gtttcaccac cggtggtctg aacttcgatg ccaaagtacg
    12841 tcgtcaaagt actgataaat atgatctgtt ttacggtcat atcggcgcga tggatacgat
    12901 ggcactggcg ctgaaaattg cagcgcgcat gattgaagat ggcgagctgg ataaacgcat
    12961 cgcgcagcgt tattccggct ggaatagcga attgggccag caaatcctga aaggccaaat
    13021 gtcactggca gatttagcca aatatgctca ggaacataat ttgtctccgg tgcatcagag
    13081 tggtcgccag gagcaactgg aaaatctggt aaatcattat ctgttcgaca aataacggct
    13141 aactgtgcag tccgttggcc cggttatcgg tagcgatacc gggcattttt ttaaggaacg
    13201 atcgatatgt atatcgggat agatcttggc acctcgggcg taaaagttat tttgctcaac
    13261 gagcagggtg aggtggttgc ttcgcaaacg gaaaagctga ccgtttcgcg cccgcatcca
    13321 ctctggtcgg aacaagaccc ggaacagtgg tggcaggcaa ctgatcgcgc aatgaaagct
    13381 ctgggcgatc agcattctct gcaggacgtt aaagcattgg gtattgccgg ccagatgcat
    13441 ggagcaacct tactggatgc tcaacaacgg gtattgcgcc ctgccatttt gtggaacgac
    13501 gggcgctgtg cgcaagagtg cactttgctg gaagcgagag ttccgcaatc acgagtgatt
    13561 accggcaacc tgatgatgcc cggatttact gcgcctaaat tgctatgggt tcagcggcat
    13621 gagccggaga tattccgtca aatcgacaaa gtattattac cgaaagatta cttgcgtctg
    13681 cgtatgacgg gggagtttgc cagcgatatg tctgacgcag ctggcaccat gtggctggat
    13741 gtcgcaaagc gtgactggag tgacgtcatg ctgcaggctt gcgacttatc tcgtgaccag
    13801 atgcccgcat tatacgaagg cagcgaaatt actggtgctt tgttacctga agttgcgaaa
    13861 gcgtggggta tggcgacggt gccagttgtc gcaggcggtg gcgacaatgc agctggtgca
    13921 gttggtgtgg gaatggttga tgctaatcag gcaatgttat cgctggggac gtcgggggtc
    13981 tattttgctg tcagcgaagg gttcttaagc aagccagaaa gcgccgtaca cagcttttgc
    14041 catgcgctac cgcagcgttg gcatttaatg tctgtaatgc tgagtgcagc gtcgtgtctg
    14101 gattgggccg cgaaattaac cggcctgagc aatgtcccag ctttaatcgc tgcagctcaa
    14161 caggctgatg aaagtgccga gccagtttgg tttctgcctt atctttccgg cgagcgtacg
    14221 ccacacaata atccccaggc gaagggggtt ttctttggtt tgactcatca acatggcccc
    14281 aatgaactgg cgcgagcagt gctggaaggc gtgggttatg cgctggcaga tggcatggat
    14341 gtcgtgcatg cctgcggcat taaaccgcaa agtgttacgt tgattggtgg cggggcgcgt
    14401 agtgagtact ggcgtcagat gctggcggat atcagcggtc agcagctcga ttaccgtacg
    14461 gggggggatg tggggccagc actgggcgca gcaaggctgg cgcagattgc ggcgaatcca
    14521 gagaaatcgc tcattgagtt gttgccgcag ctaccgttag aacagtcgca tcaaccagat
    14581 gcgcagcgtt atgccgctta tcagccacga cgagaaacgt tccgtcgcct ctatcagcaa
    14641 cttctgccat taatggcgta aacgttatcc cctgcctgac cgggtggggg ataattcaca
    14701 tctatatatc tcagtaatta attaatattt agtatgaatt tattctgaaa atcatttgtt
    14761 aatggcattt ttcagttttg tctttcgttg gttactcgta atgtatcgct ggtagatatg
    14821 gagatcgtta tgaaaacctc aaagactgtg gcaaaactat tatttgttgt cggggcgctg
    14881 gtttatctgg ttgggctatg gatctcatgc ccattgttga gtggaaaagg ctattttctt
    14941 ggcgtgttaa tgacagcaac ttttggcaac tatgcatatc ttcgcgcaga aaaactcggg
    15001 caactggata atttttttac ccatatttgc cagttagttg cgttaatcac tatcggtctc
    15061 ttgtttatcg gtgttttaaa tgcacctatc aatgcttatg aaatggtgat ctaccccatc
    15121 gccttttttg tctgcttgtt tggtcaaatg cgtttgtttc gctcggtatg agcaacataa
    15181 agctcttaca cattcaggaa tgaaaggaat actgtgatgg acaacaaaat atcaacctat
    15241 tcaccggcct ttagtattgt gtcatggata gctctcgttg gtggtatcgt tacctatctg
    15301 ttagggctat ggaatgcaga gatgcagtta aatgaaaaag gatattattt tgccgtactg
    15361 gtattaggac tgttttctgc ggcgtcttat caaaagaccg ttcgggacaa gtatgaaggc
    15421 ataccgacca cttccattta ttatatgacc tgcctgactg tctttattat ctctgttgca
    15481 ttactgatgg taggtctgtg gaatgcgaca ttattactca gcgaaaaagg tttttatgga
    15541 ctggctttct tcttaagctt gtttggtgca gtagcggtgc agaaaaatat tcgtgatgcc
    15601 ggaataaacc caccaaaaga aacacaggtt acccaggaag aatacagcga ataactcacg
    15661 taagcccggt cagtccaatg tgaccgggct tttacttaac tcactaatct gtttctgtcg
    15721 attcgttgta ccagcataga aagtaacaaa ctcgctgcca acgtcgcgca aaaaatccaa
    15781 ataatatcca gtattggcca atttttaagc tcaattcccc gggtgcgcag cgcatggata
    15841 atcaaggcgt ggaatccgta tatacccaac gaatggcggg agattaagcc aagtccgcga
    15901 atggtacgcg tatccagcgt gtttttaacc agagtcaata gcgcgattgc gcagataaaa
    15961 accatcggcc cacagtaaag ataccaggta tcggcaaaat ttccgcgcca ctgcaattca
    16021 tataatgtcc cgcgagagat aataaaaact cccgtcgcaa acagtgcggc gctcacccac
    16081 gacagtgctt tatgctgtgt gtccatcatc cctatagcgc ggcccaacat gccatacaga
    16141 atgtagtaaa aagtatcgcc attgatatat aagttaattg gcagccattc aaaaccgtcg
    16201 attttctgcg gcacggtgtt tgggttagcg atgataccaa tcaccaccat tagcaccagc
    16261 aacatttttc cgccgacgtt cttcacctga atcagcggtg aaaccagata aatcaccgca
    16321 atcgcgaaga aaaaccataa gtggtaaaac actggctttt gcagcaggtt tttcagcgct
    16381 aactccatat taatggaggt aaacagtgca atgtagagca gcgcgattgc gctataaaaa
    16441 aacagacata agccgatacg caagaaatgg cgcggctggg cgctgcgttc gccaaaaaag
    16501 agatagccgg aaatcatgaa aaatagcggc acgctgacac gagaggcaga gttcagaaca
    16561 ttggcgatat cccaggtgac ggggctaaca ctatgagcat tggtcacata ccaggtagtg
    16621 gtgtgaatca tcaccaccat taaacacgct atccctcgca ggttatcaat ccagtaaatt
    16681 ttgggctgca tctgtgtctc tgtatctggt taaaaaaagt ctgaccgata aatcattcag
    16741 ttggcgcact ggaataatct gagttttaac actacagctt atagaggctt aaggaaattc
    16801 gtaagatatc agccactata ccgatataaa taataagact cacctgcaaa ccagacggta
    16861 atttaatgat gatgaacgct ttctttccgg caatggcgct tatggtgcta gtgggttgtt
    16921 ctacaccgtc acccgtgcag aaagcacaac gggtaaaggt tgatcctctg cgttcgttga
    16981 atatggaagc gttatgcaag gatcaggcgg caaaacgtta taacaccggc gagcaaaaaa
    17041 tcgacgtcac cgccttcgaa cagttccagg gaagctatga aatgcgcggt tataccttcc
    17101 gtaaagagca gtttgtctgt tcttttgacg cagatggcca ttttttgcat ctttccatgc
    17161 gttaagccct gctttttccc gtttcgtact gtatatcttc catccagcgg gtatactgat
    17221 cccttccttt aaatccacac gtatccagca cgaaataata tgcaaaagtt tgataccagg
    17281 accttccagg gcttgatcct gaccttacag gattactggg ctcgccaggg ctgcaccatt
    17341 gttcaaccat tggacatgga agtcggcgcg ggaacctctc acccaatgac ctgtctgcgc
    17401 gcgctggggc cagaaccgat ggcggctgct tatgttcagc cttctcgtcg cccgaccgat
    17461 ggtcgctacg gcgaaaaccc caaccgttta cagcactact atcagttcca ggtggtcatt
    17521 aagccatcgc cggacaatat tcaggagctg taccttggtt ctctgaaaga gctgggcatg
    17581 gacccgacta tccacgatat ccgtttcgtg gaagataact gggaaaaccc gacgctgggt
    17641 gcctggggac tgggctggga agtgtggcta aacggcatgg aagtgacgca gttcacttac
    17701 ttccagcagg ttggtggtct ggagtgtaaa ccggttaccg gcgagatcac ctacggtctg
    17761 gaacgtctgg ccatgtacat tcagggcgta gacagcgttt acgacctggt ctggagcgac
    17821 ggcccgctgg gtaaaaccac ctacggcgac gtgttccatc agaacgaagt ggagcagtcc
    17881 acttacaact tcgaatacgc ggacgtggac ttcctgttca cctgcttcga gcagtacgag
    17941 aaagaagcgc agcagttgct ggcgctggaa aatccgctgc cgctgccagc ctacgagcgt
    18001 attctgaaag ccgcccacag cttcaacctg ctggatgcgc gtaaagccat ctccgtcact
    18061 gagcgtcagc gctacattct gcgcattcgc accctgacca aagcagtggc agaagcatac
    18121 tacgcttccc gtgaagccct cggcttcccg atgtgcaaca aagataagta agaggcggct
    18181 atgtctgaga aaacttttct ggtggaaatc ggcactgaag agctgccacc aaaagcactg
    18241 cgcagcctgg ctgagtcctt tgctgcgaac tttactgcgg agctggataa cgctggcctc
    18301 gcacacggca ccgttcaatg gtttgctgct ccgcgtcgtc tggcgctgaa agtagctaac
    18361 ctggcggaag cgcaaccgga tcgtgaaatc gaaaaacgcg gcccggcgat tgcccaggcg
    18421 ttcgacgctg aaggcaaacc gagcaaagcg gcagaaggtt gggcgcgtgg ttgcggtatt
    18481 accgttgacc aggctgagcg tctgactacc gataaaggtg aatggctgct gtatcgcgcc
    18541 cacgtgaagg gcgaaagcac tgaagcactg ctgccgaata tggttgcgac ttcgctggcg
    18601 aaactgccga ttccgaaact gatgcgctgg ggtgcaagcg acgtgcactt cgtgcgtccg
    18661 gttcacaccg tgaccctgct gctgggcgac aaagtcattc cggcaaccat tctgggcatt
    18721 cagtccgatc gcgtgattcg cggccaccgc tttatgggcg agccggaatt caccatcgat
    18781 aacgccgatc agtatccgga aattctgcgt gagcgcggga aagtcatcgc cgattacgaa
    18841 gaacgtaagg cgaagattaa agccgatgcc gaagaagcag cgcgtaagat tggcggtaac
    18901 gctgacttaa gcgaaagcct gctggaagaa gtggcttcgc tggtggagtg gccggtcgtt
    18961 ctaaccgcga aatttgaaga gaaattcctc gcggtgccgt ctgaagcact ggtttacacc
    19021 atgaaaggtg accagaagta cttcccggtg tatgcgaacg acggcaaact gctgccgaac
    19081 tttatcttcg ttgccaacat cgaatcgaaa gatccgcagc aaattatctc tggtaacgag
    19141 aaagtcgttc gtccacgtct ggctgatgcc gagttcttct tcaacaccga ccgtaaaaaa
    19201 cgtctggaag ataacctgcc gcgcctgcaa accgtgctgt tccagcaaca gctgggtacg
    19261 ctgcgtgaca agactgaccg catccaggcg ctggctggct ggatcgccga acagattggc
    19321 gctgacgtta accacgcaac ccgtgcgggc ctgctgtcta agtgtgacct gatgaccaac
    19381 atggtcttcg agttcaccga cacccagggc gttatgggga tgcactacgc gcgtcacgat
    19441 ggcgaagcgg aagacgtagc cgtcgcgctg aatgagcagt atcagccgcg ctttgccggt
    19501 gatgacctgc cgtccaaccc ggtagcttgt gcgctggcga ttgctgataa gatggatact
    19561 ctggcgggta tcttcggtat cggtcagcat ccgaaaggcg acaaagaccc gtttgcgctg
    19621 cgtcgtgccg cacttggcgt gctgcgaatt atcgttgaga agaacctcaa ccttgatctg
    19681 caaacgctga ccgaagaagc ggtgcgtctg tatggcgata agctgactaa tgccaacgta
    19741 gttgatgatg ttatcgactt tatgctcggt cgcttccgcg cctggtatca ggacgaaggt
    19801 tataccgttg acaccatcca ggcggtactg gcgcgtcgtc cgactcgtcc ggctgatttc
    19861 gatgcccgca tgaaagcggt atcgcacttc cgtaccctgg aagcagctgc agcactggcg
    19921 gcggcgaaca aacgtgtatc taacatcctg gcgaaatctg acgaagcgct gagcgatcgc
    19981 gtgaatgcct ctactctgaa agagccggaa gaaattaaac tggcgatgca ggttgtggtg
    20041 ctacgtgaca agctggagcc gtactttgcg gaaggtcgtt accaggatgc gctggtcgaa
    20101 ctggcagagc tgcgtgaacc ggtcgatgcc ttcttcgata aagtgatggt catggttgat
    20161 gacaaagaat tgcgtatcaa ccgtctgacc atgctggaga aactgcgcga actgttcctg
    20221 cgcgttgcgg atatttcgct gttgcagtaa taacgccgtt attaaaaagc ctgccatctg
    20281 gcaggctttt tttatcgcta aataatagag caacctttaa tactcttctg ctgaataaag
    20341 attatctcat atattaat
//
LOCUS       DAPGWD010000067        20293 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPGWD010000067 DAPGWD010000000
VERSION     DAPGWD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.067"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..1486
                     /locus_tag="RHX88_002466"
     CDS             32..1486
                     /locus_tag="RHX88_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDV2231141.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            1546..2907
                     /gene="allB"
                     /locus_tag="RHX88_002467"
     CDS             1546..2907
                     /gene="allB"
                     /locus_tag="RHX88_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDV2231142.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            2964..4265
                     /locus_tag="RHX88_002468"
     CDS             2964..4265
                     /locus_tag="RHX88_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDV2231143.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSELGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            4287..5432
                     /gene="glxK"
                     /locus_tag="RHX88_002469"
     CDS             4287..5432
                     /gene="glxK"
                     /locus_tag="RHX88_002469"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDV2231144.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(5561..6346)
                     /gene="allE"
                     /locus_tag="RHX88_002470"
     CDS             complement(5561..6346)
                     /gene="allE"
                     /locus_tag="RHX88_002470"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDV2231145.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(6357..7592)
                     /gene="allC"
                     /locus_tag="RHX88_002471"
     CDS             complement(6357..7592)
                     /gene="allC"
                     /locus_tag="RHX88_002471"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDV2231146.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(7614..8663)
                     /gene="allD"
                     /locus_tag="RHX88_002472"
     CDS             complement(7614..8663)
                     /gene="allD"
                     /locus_tag="RHX88_002472"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDV2231147.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            8980..10647
                     /locus_tag="RHX88_002473"
     CDS             8980..10647
                     /locus_tag="RHX88_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV2231148.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            10657..11916
                     /locus_tag="RHX88_002474"
     CDS             10657..11916
                     /locus_tag="RHX88_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV2231149.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            11927..12742
                     /locus_tag="RHX88_002475"
     CDS             11927..12742
                     /locus_tag="RHX88_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV2231150.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            12739..13632
                     /locus_tag="RHX88_002476"
     CDS             12739..13632
                     /locus_tag="RHX88_002476"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV2231151.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13827..14894)
                     /gene="purK"
                     /locus_tag="RHX88_002477"
     CDS             complement(13827..14894)
                     /gene="purK"
                     /locus_tag="RHX88_002477"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDV2231152.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFG"
     gene            complement(14891..15400)
                     /gene="purE"
                     /locus_tag="RHX88_002478"
     CDS             complement(14891..15400)
                     /gene="purE"
                     /locus_tag="RHX88_002478"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDV2231153.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(15518..16240)
                     /gene="lpxH"
                     /locus_tag="RHX88_002479"
     CDS             complement(15518..16240)
                     /gene="lpxH"
                     /locus_tag="RHX88_002479"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDV2231154.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(16243..16737)
                     /gene="ppiB"
                     /locus_tag="RHX88_002480"
     CDS             complement(16243..16737)
                     /gene="ppiB"
                     /locus_tag="RHX88_002480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDV2231155.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            16911..18296
                     /gene="cysS"
                     /locus_tag="RHX88_002481"
     CDS             16911..18296
                     /gene="cysS"
                     /locus_tag="RHX88_002481"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDV2231156.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(18332..18853)
                     /locus_tag="RHX88_002482"
     CDS             complement(18332..18853)
                     /locus_tag="RHX88_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2231157.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(18961..19173)
                     /gene="ybcJ"
                     /locus_tag="RHX88_002483"
     CDS             complement(18961..19173)
                     /gene="ybcJ"
                     /locus_tag="RHX88_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDV2231158.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(19175..20041)
                     /gene="folD"
                     /locus_tag="RHX88_002484"
     CDS             complement(19175..20041)
                     /gene="folD"
                     /locus_tag="RHX88_002484"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDV2231159.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
BASE COUNT         4633 a         5152 c         5585 g         4923 t
ORIGIN      
        1 gttattcgat acgtgaaatt aagagggatt tatggaacat cagagaaaac tattccagca
       61 acgcggctat agcgaagatc tattgccgaa aacgcaaagc cagcggacct ggaaaacatt
      121 taactatttt accttatgga tgggttcggt tcataacgtt cccaattatg tgatggtcgg
      181 cggctttttt attctcggct tgtctacctt tagtattatg ctggcaatta tcctcagcgc
      241 ctttttcatt gccgcggtaa tggtattaaa cggcgctgcg ggcagtaaat acggcgtacc
      301 gtttgccatg atcctgcgtg cttcttacgg cgtacgtggc gcactgtttc ccggattatt
      361 aagaggcggt attgctgcca ttatgtggtt tggcctgcaa tgttacgcgg ggtcactggc
      421 ctgcttgatt ctgattggca aaatctggcc gggattttta actctcggtg gtgatttcac
      481 gctgttaggg ctttctctac cgggcttaat tactttctta ctcttctggc tggtcaacgt
      541 tggtataggt tttggcggtg gcaaagtttt aaataaattc actgccattc ttaacccgtg
      601 catctatatc gttttcggcg gcatggcgat ttgggcgatt tcactggtcg ggatcggtcc
      661 aatctttgac tacattccga gcggtattca gaaagcagaa aacagtggat tcttgttcct
      721 ggtggtgatt aacgcggtag ttgcggtctg ggcggcaccg gcggtgagcg catccgactt
      781 tacgcaaaac gcccactcgt ttcgtgagca agcgctgggg caaacgctgg gtttagttgt
      841 ggcctatatt ctgtttgcgg tcgctggggt atgtattatt gccggagcca gtattcacta
      901 cggcgctgac acctggaacg tgctggatat tgttcagcgt tgggacagcc tgtttgcctc
      961 gttctttgcg gtactggtta ttctgatgac gactatttcc actaacgcca ccggtaatat
     1021 tattccggcc ggttatcaga ttgctgccat tgcaccgaca aaactgacct ataaaaacgg
     1081 cgtactgatt gccagtatta tcagcctgct gatctgcccg tggaaattaa tggaaaatca
     1141 ggacagtatt tatctgttcc tcgatatcat cggcggaatg ctgggtccgg taattggtgt
     1201 catgatggca cattattttg tggtgatgcg tgggcaaatt aatcttgatg aactgtacac
     1261 cgcacctggc gattataaat attacgataa cggttttaac ctcaccgcgt tttcagtaac
     1321 tctggtggcc gttattttat ctcttggcgg taagtttatt ccctttatgg aaccgttatc
     1381 gcgtgtttca tggtttgtcg gcgtcatcgt cgcctttgcg gcctacgcct tattaaagaa
     1441 acgtacaaca gcagaaaaaa caggagagca aaaaaccata ggttaattaa tcccgatatt
     1501 gaacattgag ttaaaaacca atctgtattt tacaaggagt ttgttatgtc ttttgattta
     1561 atcattaaaa acggcaccgt tattttagaa aacgaagctc gcgttgtaga tatcgccgtt
     1621 aaaggcggga aaattgctgc tatcggtcag gatctgggcg atgcaaaaga agttatggat
     1681 gcgtctggtc tggtggtttc gccgggcatg gttgatgcgc acacccatat ttctgaaccg
     1741 ggtcgtagcc actgggaagg ttatgaaacc ggtactcgcg cagcggcaaa aggtggtatc
     1801 accaccatga tcgaaatgcc gctcaaccag ctgcctgcaa cggttgaccg cgcttcaatt
     1861 gaactgaagt tcgatgccgc taaaggcaag ctgactatcg atgcggcaca actcggtggc
     1921 ctggtgtctt acaacatcga ccgtctgcat gagctggatg aagtgggcgt tgtcggcttc
     1981 aaatgcttcg ttgcgacctg tggcgatcgc ggtatcgaca acgacttccg tgatgtaaac
     2041 gactggcagt tcttcaaagg tgcgcagaag ctgggcgaac tggggcagcc ggtgctggtg
     2101 cactgcgaaa acgcgctgat ctgtgacgca ctgggcgaag aagcgaaacg tgaaggtcgc
     2161 gtaactgccc atgactatgt ggcttcgcgt ccggtattta ccgaagtgga agcgattcgc
     2221 cgcgtgctgt atctggcgaa agttgccggt tgccgtctgc acgtttgcca tatcagcagc
     2281 ccggaaggcg ttgaagaagt gactcgtgca cgtcaggaag gtcaggacgt tacttgtgaa
     2341 tcctgcccgc attactttgt actggatacc gatcagttcg aagaaatcgg cactctggcg
     2401 aagtgttcac cgccgatccg cgatctggaa aaccagaaag gcatgtggga aaaactgttt
     2461 aacggtgaaa tcgactgcct ggtttccgac cactcaccat gccctccgga aatgaaagcc
     2521 ggcaacatca tggaagcatg gggcggtatt gccggtctgc aaaactgtat ggacgtgatg
     2581 ttcgatgaag cggtacagaa acgcggaatg tctctgccaa tgttcggcaa attaatggcg
     2641 actaacgcag cagatatttt cggtctgcag caaaaaggcc gtatcgcccc aggaaaagat
     2701 gccgacttcg tcttcattca gccgaatagc agctatgttc ttaccaatga cgatctggaa
     2761 tatcgccaca aagtcagccc gtatgttggc cgtaccattg gcgcgcgtat cacgaaaacc
     2821 atcttacgtg gtgatgtgat ttacgacatt gaacagggct tccctgttgc gccgaaaggt
     2881 caatttatcc ttaaacatca gcagtaatct ggcccctgca atgcccgtcc ttgcggcggg
     2941 cattctccgg ttaaggtgtg tttatgttca attttgcagt cagccgcgaa agcctgttat
     3001 caggatttca gtggtttttc tttatttttt gcaacacggt tgtggttcct cctacgctac
     3061 tttctgcttt tcagttgccg caaagtagcc tgcttacgct cacgcaatat gcttttcttg
     3121 ctaccgcact ggcctgcttc gctcaggcgt tctgcggtca tcgtcgcgct attatggaag
     3181 ggccgggtgg cctgtggtgg ggaaccatcc ttactatcac ccttggcgaa gcatcgcgcg
     3241 ggacaccgat caacgatatc gctaccagcc tggcggtggg gattgcgctc tccggcgtgc
     3301 tgacgatgtt gattggtttt agcgaattag gccatcgcct ggcacggtta tttacgccgt
     3361 cggtgatggt cttgtttatg ttgatgctgg gcgcgcagct gaccactatc tttttcaaag
     3421 gtatgctcgg gctgccgttt ggcatagccg acccaaattt taaaattcag ttaccgccgt
     3481 tcgcgctctc ggtggcggtg atgtgcctgg tactggggat gattatcttc ctgccgcaac
     3541 gttttgcccg ttatggcctg ctggtcggca ccataaccgg ctggttgttg tggtactttt
     3601 gctttccttc ttcgcactcg ctctccggtg agttgcactg gcagtggttc ccgctcggca
     3661 gtggaggtgc tttgtctccg ggaattattc tgacggcggt gattacaggt ctggtgaata
     3721 tcagcaatac ctacggtgcg attcggggca cggatgtttt ttatccgcag cagggcgcgg
     3781 ggaatacgcg ttatcgtcgc agctttgtgg cgaccggatt tatgacgctg ataaccgtac
     3841 cgctggcggt aattccattt tcaccgtttg tttcatccat tggtttatta acccagacag
     3901 gcgattacac gcggcgttcg tttatttatg gcagcgttat ttgccttctg gtggcgctgg
     3961 ttccggcact cacacgactg ttttgcagta tccctttacc cgtgagtagt gcggtcatgc
     4021 tggtttctta tctgccttta ctcttttccg cgctggtgtt tagccagcaa ataacgttta
     4081 ccgctcgcaa tatttatcga ctcgcattgc cgttatttgt cggcatattt ttaatggcat
     4141 taccgcctgt gtatctgcaa gaccttccat taacgctgcg tcctctgctc agtaacggct
     4201 tactggtcgg gattttactg gctgttctta tggataatct tattccgtgg gaacgcatcg
     4261 aataatttgt tgaaaaagga ttgataatga agattgtcat tgcgccagac tcttttaaag
     4321 agagcttaag tgcagaaaaa tgttgtcagg caattaaagc cgggttttcg accctctttc
     4381 ccgatgcgaa ctatatctgt ttgccgatag cggatggcgg cgaagggacg gtggatgcga
     4441 tggtcgccgc gacgggcggc aacatcgtga cgcttgaagt ctgcgggccg atgggcgaaa
     4501 aagtgaatgc tttttatggc cttaccggcg acgggaaaac ggcggtgatt gagatggcgg
     4561 cagcaagtgg cctgatgctg gtcgcgcctg aaaagcgtaa tccgttgctg gcctccagtt
     4621 ttggtacggg ggagttaatt cgtcatgcgc tggataacgg cattcgccat attattctcg
     4681 gcattggcgg cagtgcgacg gtcgacggtg gtatgggcat ggcgcaggcg ctcggtgtgc
     4741 gtttccttga tgccgacggt caggtgctgg tggctaacgg cggtaattta gcgcgcgtgg
     4801 caagcattga gatggatgaa tgcgatccgc gtctggcgaa ttgccatatt gaagtagcat
     4861 gtgacgttga taacccgctg gtaggggcac gcggcgcggc ggcggtgttt ggcccgcaaa
     4921 aaggggcaac gccggaaatg gtcgaagagc tggaacaggg gctgcaaaat tacgcccgtg
     4981 ttttacaaca gcaaactgaa atcaacgtct gccagatggc gggcggcggc gctgcgggcg
     5041 gtatgggtat tgcggcggcg gtatttctca atgcggatat taaaccaggt attgagattg
     5101 tgttgaatgc ggtcaatctt gcgcaggcag tgcagggcgc agcactggtg attaccgggg
     5161 aagggcgcat cgactcgcaa acggcaggcg gtaaagcgcc gctgggtgtg gcgtcggtgg
     5221 cgaagcagtt taatgtaccg gtgattggga ttgctggcgt attgggtgat ggcgtggaag
     5281 tggtgcacca gtacggcatt gatgcggtat tcagcatttt gccacgtctg gcacctttag
     5341 ccgaagtgct cgccagcggt gaaaccaatc tcttcaacag cgcacgaaat attgcctgcg
     5401 ccattaaaat aggtcaggga attaaaaact gacccttacc tttaaagcgg atgcgattta
     5461 tatcgcatct ttttttacct gaatttacta caaaaaaagc cacggtataa accgtggcaa
     5521 aatccaacat agctaaaaat aatcaggcga gtggtatgac ttaaatctcg acgtcgcggt
     5581 tacaatcttt cgagtaaata tagctgaacg cttcaccacg ccctacgcca taaccagcct
     5641 gtaaagaata agcgcccata aagatgtaat cgcctttttt caccgggatc cagttattgt
     5701 cgaggttata aaccccctga ccggaaagaa tataggcacc gtgttcctga acatgtgttt
     5761 cgatataacc gtggctggca cctggtgcaa aagagaggat atgcatgttc atatcaaaac
     5821 ctaactcttt gggcaggaaa tccagcagga tcacatcatc cataccttca tagtgaatgc
     5881 gttccagttc gctggcatta ccagaaacca gccacggtgc atggccttct accggaacat
     5941 agcggcgctt atataaaaag atttggctgt cttcggcctg ggcgttaaca aacgtcatta
     6001 aggagcctgg tgggcaataa agatagccac cttcgcttaa ggcaaatgtt ttgccttcgg
     6061 ctttggcagt gatatttcca gagatcacat acaggaacgt ttcaatgcct tcgccaccga
     6121 agccctgttg gttgccaccg ttttgatgca gtgtgaccag ataatcaaca aaagaggcac
     6181 ccagctttgg cgtggagagg attgtcgcgt cacaattttc aaagcccgga ataatatttt
     6241 ttaccagacc atccggggtt aacagtgcga aattaccgtg tttaacaatc gcacggttag
     6301 ccagtaaatc atcgcggtaa ccggtgacgt tatttaaata tcccatttat gactccttat
     6361 ttctgccagg caagttgata aagcatgagt gccaacgttt tgaccccttc ggcaaggtcg
     6421 gtaatattgg tgcgttccgc cgggttatgg ctgatcccgt tgatgctggg aatgaaaatc
     6481 atgcaggttg gtacgcgagg cgcgaaaatt tgcgcgtcgt gcccggcacc actgtgcatc
     6541 acccggtaat tcagtttttc tctttcacac aattctgtca gggtggcgac cagctcctta
     6601 ttcatcggca cgggttcttc gtccatccat aaatcgatat caataccaat gtccatttca
     6661 tcgcaaatcg cccgcatgtc gttttctaac tgttgggtga aatcgcgcag cacggcagcg
     6721 tcggtatgac gacaatcaat ggtgaacgtg gttttacccg gcaccacatt taccgtattc
     6781 gggcgcggct ctactttgcc aaaggtcaga accagcggat cgcccatcct tttcgctttt
     6841 tcgaccgact gatggcaaat gcgactgaaa gcgtaaactg tatcacgacg ataacccatc
     6901 ggcgtggtgc ctgcatggtt tgattcgccg ttcagcgtta ccgtataacg acgctgcccg
     6961 acaattgcat tcaccacgcc aattgattgc ccattacttt ccagcacaca gccctgttca
     7021 atatgcagtt caacaaaggc tttaatatcc tgacgcggag ttagtggggc gttcggaaga
     7081 gtaaatccgc aagccttcat cgcatcgaca aaactatttc ctttggcatc acagatattc
     7141 cgcacgtcgt caggattcgc cagcccaaag atatttttac tgccccagaa gacatacggg
     7201 aagcggctgc cttcttcttc tgccatcgcc accacttcga ccgtacgtag cggcgcgccg
     7261 tattgcgttt tcagccagtc aattgccagc cacgccgcca gcgcgccgaa ttgcccgtcg
     7321 aggttaccgc cgttaaccac ggtatcgata tgcgaaccgc tcagaaccac ttcttgtgga
     7381 tattcggtgc cactcaggcg accatataaa ttccccactt catcgaaacg tgtttccagc
     7441 ccgcttgctg ccattctttt tttaaattgc tgctgagttt ccagccattc cggcgaataa
     7501 agtaaacggg tcataccacc cgttgggtca gcgccaaaag aggaaagcca gggcagcgtt
     7561 tcttctatgg cttgacgaaa atgtgtaatc ataagaaagt cctgtctcaa taattattgc
     7621 gcaaagggat ttttcgtttc gtatgacgtg ttataaagcg cgtcggaaat taaatactgg
     7681 taaatatcat caacaatttc gatgccttcg acggcggctt tgcgttgttt aatatcctga
     7741 tcctgtccgg gataataaac ctgattaaaa ccgggcgcgg gggtaatggc atttaattcg
     7801 cgcatggtct ggctaagatg ttgacggaat aattcgctgg agaagaaaaa gttcgggtta
     7861 ataacgatat gtaattgccc caaattacgc ccggcgtgta aatcgtcata catcgaacta
     7921 acctgtcgcc cgaacggtaa gccgagtaag acgcctgaga ggacgtcaat catcatcatc
     7981 aggccatacc cttttggccc ggcggcgggg agcagagcat gtaccgcgaa cggatcggtt
     8041 gttggtgcac cgtttttatc gaccgcccag gtatccggga tagacatatt acgcgagcgg
     8101 gcgtcgagca cttttcccca tgcctgtacg gtagtcgcca tatcaaaggt aaggatctcg
     8161 tcgccttctc ccggcgcggc aaaggccagt gggttagtac cgtagtaaat ttccgcgccg
     8221 ccaaacggca ccaccattgg atcggactgg cacatcgaaa taccaattaa tccggcgcgg
     8281 gctgcctgct gcacaaaata agagattgcg ccgctgtgac ccatccggct gataccgacc
     8341 accgcaacgc cattttgctg ggcggttttg atggcatgtt ccatacccat tttcgccgcg
     8401 acctgtccgg cggcattgtc ggcatgtaaa attgccgagc acggcccggt ttcctcaaga
     8461 cgaaactccg gttcgcggtt ggtgccgcct tttgaaatgc gttccgcgta gtattccacg
     8521 cgcaccgcgc catgagagtg gatccctctg gcatcggcgt aaaccaatac ttcagccacg
     8581 gttgcagcgt gctcacgttt taacccagcc tggcagagtt tattctcaat tagctggtgg
     8641 agtgtttccc gactgatttt catctgtctt cctttttaac gacggtgtga agcatgactg
     8701 caattaacat acagggaaaa tatctggatt atgtgatcca gacaggcaaa aaaatatagt
     8761 tagaatttat ttgataatcc gctcactttt aacctgattt ttaaaacaac cacgcttatt
     8821 aaaaaataat gcgtaatagc ctggcggtta tttgaattct tttgttaata attcctgtgt
     8881 gatattcatc accttattta cttgttgtca tcgataccgt aatcgccaca ttaacactgc
     8941 tcgtgcaatt gccatgggtg caatttttaa ggagttgtta tgatccacgc ctttattaaa
     9001 aaagggtgtt ttcaggattc ggtcagttta atgattattt cacgaaaact cagcgaatca
     9061 gaaaatgttg atgatgtttc cgtaatgatg ggtacgcccg ccaataaagc gttattagat
     9121 accacaggtt tctggcatga cgattttaat aacgccacgc cgaacgatat ttgcgtggca
     9181 attcgtagcg aagcggcgga tgcggggatc gcgcaggcga ttatgcagca gcttgaagag
     9241 gcgctaaaac aactggcgca ggggtcaggc agcagccagt ccttgacgca ggtgcgtcgc
     9301 tgggacagcg cctgtcagaa attacccgat gccagtctgg cgctgatttc agtggctggc
     9361 gagtatgcgg cggagctggc gaaccaggcg ctggatcgca acctcaacgt gatgatgttc
     9421 tccgataacg tcacgctgga agatgaaatc caactcaaaa cccgcgcgcg ggaaaaaggc
     9481 ttgctggtga tggggccgga ctgcggtaca tcgatgattg ccgccacgcc gctggctttt
     9541 gctaacgtga tgccggaagg caacattggc gtcattggcg cttccggtac cgggattcag
     9601 gagctgtgct cacagattgc gctggcaggg gagggaatta ctcacgcgat tggccttggc
     9661 gggcgcgacc tcagccgtga agtgggcggc atcagtgcgc tgacggcgct ggaaatgctc
     9721 agtgcagacg agaaaagcga agtgctggca tttgtttcaa aaccgcctgc cgaagctgtg
     9781 cgtctgaaaa ttgttaatgc catgaaagca accggcaaac cgacggtggc gctgttttta
     9841 ggttataccc cggcggtggc ccgcgacgag aatgtctggt ttgcctcctc gctggatgag
     9901 gccgcacgcc tggcttgcct gctttcacgc gttacggcgc gacgtaacgc gataacgcct
     9961 gtcagcagcg gatttatttg cggtttgtat accggcggta cgctggctgc cgaagcggcg
    10021 ggattacttg ctggacacct tggcgtggaa gccgacgata cccatcatca cggcatgatg
    10081 ctggacgccg atggtcacca gatcattgat ctgggcgatg atttctacac cgtcgggcgt
    10141 ccccatccga tgatcgaccc agccttacgc aaccagttaa ttgccgatct cggcgctaaa
    10201 ccgcaagtgc gcgtgttgct gcttgatgtc gtgattggct tcggtgcgac cgccgatcct
    10261 gctgcctcgc tggtgagcgc ctggcaaaaa gcctgtgccg cgcgttcaga cagccaaccg
    10321 ctgtacgcca ttgccacggt gacaggtacc gaacgtgacc cgcaatgccg ctcgcagcaa
    10381 atcgccacgc tggaagatgc agggatcgcg gtagtgagtt cgctgccgga agccaccttg
    10441 ctggcggcgg cactgattcg tccgctttcg cctgccactc agcaacacac accgtcatta
    10501 ctggaaaacg tcgccgtgat taacatcgga ttacgcagct ttgcgctgga gctacaaagc
    10561 gccagcaaac cggttgtgca ttaccaatgg tcgccagtcg ccggcggcaa taaaaaactg
    10621 gctcgtttat tagaacgttt gcaataaggg gttcccatgt ttacatcagt ggcgcaagcc
    10681 aatgctgcgg ttatcgaaca aattcgtcgc gctcgtccac actggctgga tgtgcaaccg
    10741 gcttcttcac ttatcagcga actaaacgag ggcaaaacac tgcttcacgc cgggccgcca
    10801 atgcgctggc aggagatgac cggacccatg aaaggggcgt gcgtgggcgc atgtctgttc
    10861 gaaggctggg cgaaagatga agcgcaggcg ctggcaatac tggagcaggg ggaagtgaac
    10921 ttcattcctt gtcaccatgt gaatgccgtc ggtccaatgg gcggtattac ttctgccagt
    10981 atgccgatgc tggtggtcga gaacgtgacc gacggcaacc gggcgtactg caacctcaac
    11041 gaaggtatcg gcaaagtgat gcgttttggc gcttacggcg aagatgtcct gactcgccat
    11101 cgctggatgc gcgatgtgtt aatgccagta ttaagcgcgg cgctggggcg catggagcgc
    11161 ggtatcgatc tcacggcgat gatggcgcag ggcattacga tgggcgatga gttccatcaa
    11221 cgcaatattg cttcctctgc actgttaatg cgtgcgctgg ccccacaaat tgctcgcctc
    11281 gatcatgata aacagcacat cgccgaagtg atggatttcc tcagcgtgac cgatcagttc
    11341 ttcctcaacc tcgcgatggc ttactgcaag gcggcgatgg atgctggcgc gatgatccgc
    11401 gcaggcagca tcgtcacggc aatgacccgc aacggcaata tgttcgggat tcgggtaagc
    11461 gggctgggcg aacgctggtt tactgcgcct gtaaacactc cgcaaggtct gtttttcacc
    11521 ggcttctcgc aggagcaggc gaacccggat atgggcgata gcgcgattac cgaaaccttt
    11581 ggtatcggag gtgcggcaat gatcgcagcg cctggcgtaa cgcgctttgt cggtgcgggt
    11641 ggcatggaag cggccagagc ggtatctgaa gagatggcgg aaatttacct tgagcgcaat
    11701 atgcagttgc agatccctgg ctgggatttt cagggcgcgt gcctgggact ggacattcgt
    11761 cgcgtggtag aaaccggcat tacgccactc atcaataccg gtatcgccca taaagaggcg
    11821 gggatcgggc agattggcgc aggcactgtg cgggcaccgc tggcgtgttt tgaacaggcg
    11881 ctggaagcac tggctgaaag catggggatt ggttgaggaa cgcgcaatga cgatcatcca
    11941 tcctctgctt gccagccgta gtgcaccgaa ttatcgccag tcctggcggt tagcgggagt
    12001 gtggcgacgg gcgattaacc tgatgacgga aagcggcgaa ctgttaacgc tgcatcgtca
    12061 gggtagtggt ttcggccccg gaggatgggt gcttcgccgc gcgcaattcg atgcgttatg
    12121 cggtggatta tgcggcaatg aacgaccaca ggttgtggct caagggattc gcctcgggcg
    12181 tttcacggtt aaacagccac agcgttattg tttgctgcgt attacgccgc cttcgcatcc
    12241 tcaaccgctt gcagctgcat ggatgcaacg cgcggaggaa accggacttt tcggaccact
    12301 ggcgatggcg gcaagcgatc cgctgcccgc tgaattacgc cagtttcgtc actgttttca
    12361 ggccgcgctc aatggcgtta agaccgactg gcggcactgg ctgggtaaag gccccggatt
    12421 aacgccaagt catgatgaca cgttgagcgg aatgctgctg gcggcctggt attatggcgc
    12481 tttagatgcg cgcgccggtc gcccgttttt tgcctgttcc gacaatcttc aactcgttac
    12541 cacagcggtg agcgtcagtt atttacgtta tgccgcgcaa ggatatttcg cctcgccact
    12601 cctgcacttt gtccatgctc tgagttgccc gaagcgtacc gctgttgcga ttgattcgct
    12661 gctggcgctg gggcatacgt caggggcaga tacgctgctg gggttctggc ttggccaaca
    12721 attattacaa ggaaaaccat gaaaacactg gttgtagctc ttgggggcaa cgccttactc
    12781 cagcgcggtg aggcgctgac ggcggaaaat caatatcgca atatcgccag tgctgtaccc
    12841 gcgctggcac gcctggcccg ttcttatcgg ctggcgattg ttcacggcaa cgggccgcag
    12901 gtggggctgc tggcgttaca gaatctggcg tggaaagagg tagaaccgta tccgctggat
    12961 gtgctggtgg cggaaagcca ggggatgatt ggctatatgc tggcgcagag cttgagcgca
    13021 cagccgcaga tgccgcctgt tacgacggtg ctgacgcgca ttgaggtttc gcctgatgat
    13081 ccggcgtttt tgcagccaga gaaatttatt ggtccggttt atcagccaga agaacaagag
    13141 gcactggaag cggcttacgg ctggcagatg aaacgtgatg gtaaatattt gcgccgggtg
    13201 gtggcgtctc cgcaaccgcg taaaattctc gacagcgaag ccatcgagtt gttgctcaaa
    13261 gaggggcatg tggtgatttg cagtggcggc ggcggtgtgc ctgtgacgga agatggggca
    13321 gggagtgaag cggtgattga taaagatctc gctgctgcgt tgctcgctga gcagattaac
    13381 gccgatgggc tggtgatcct cactgatgct gatgcggtat atgaaaactg gggaacgccg
    13441 cagcaacgcg ccattcgcca tgccacaccg gatgagttag cgccatttgc caaagccgat
    13501 ggttcgatgg ggccgaaggt aacggcggtg agtggttatg tcagaagccg tggtaaaccc
    13561 gcgtggattg gggcgttatc gcgaattgaa gagacgctgg cgggcgaagc ggggacctgt
    13621 atttcgctgt agtcgtaggc attagacatt tgtgcctgat gcgacgcttg acgcgtctta
    13681 tcaggcctac aaccggtgcc gcatccggca attggtgcac aatgcctgat gcgatgcttg
    13741 acgcatctta tcaggcctac aatgggtacc ggatcggtag gccggataag gcgtttacgc
    13801 cgcatccggc aagaatagag caccagttaa ccgaacttac tctgcgccca aatcacgccg
    13861 ctggcatatt ccggcggcag cagcgggatt aaggcttcca gcgtcgcagt cagacgcgat
    13921 gtgtcgctgt cggtcaaatt cagatgcccc actttacgcc ccggacggac ttctttgtcg
    13981 taccagtgca gatgcaccag cggcagtttc agccagtcat aattcgcatc gctaccaatc
    14041 agattgatca tcaccgacgg attattcacc actggttgcg gtaacggcag atcggtaatc
    14101 gcccgcagat gcagctcaaa ctggctgatg ctggcaccgt tttgtgtcca gtgaccgctg
    14161 ttatgcacac gcggtgccag ttcgttgatc aacagacctt gcggggtgac aaaacactcc
    14221 atcgccatca cgcccacata gcccagctcc tgcataatcg ccgaaagcat ctcttcggct
    14281 tgcgcttgct gctgcgcatt ggcctgcggg aaagcgacgc tggtgcgcaa aataccgtcc
    14341 tgatgcaggt tatgcgtcag cggataaaac acggtactgc catcaaagcc gcgcgcgcca
    14401 accagcgaca cttcaccaga gaagtttatg ccctgctcga caatacattc gccgtagcat
    14461 tcagccggta actgctcggt ttcatcggcg cgtaaacgcc attgaccgcg accgtcatag
    14521 ccaccagtgc gacgcttaac aatcgccagc tcacctaaac gatcaaacac cgcaggccac
    14581 tcgctgcgat cggcaagtaa ctgccacggc gcggtcggca ggtggagctt atcgaaaagc
    14641 tgcttctgag tcagacggtc agcaataatc gggaacacat cgcggttcac aaaggccgga
    14701 tgacgcgcca gctcgcgggt taatgcggtt tccggccagc gttcgatctc agcggtaatc
    14761 acgctttgtt gaaaaggcac cgccgccggt tcagcgtcca gcccgaccgg ccagacagca
    14821 atgcctaacg gttcgcctgc ctgacgcagc atacggccta actgcccgtt accgaggacg
    14881 caaacctgtt tcatgccgca cctcgcgggt ccgggttttc cagcacttcg tcggtctggg
    14941 ctttgcgcca gtcattcaga cgctggtgta gctctttatc gtgagtcgcg agaatttgtg
    15001 ctgccagtaa tgccgcgttt gccgcgccag ctttaccaat cgccagcgta cccaccggaa
    15061 tgccgcgcgg catttgtacg atggagtaga ggctatcgac accgctcagt gcggcgctct
    15121 gtactggcac gcccagcacc ggcaccagcg ttttggcggc aatcatgcct ggcaggtgcg
    15181 ctgcgccacc tgcgcccgca ataatcacct gataaccgtt ctcttcggcg ctttcggcga
    15241 agctgaacag tttatcgggg gtgcggtgag cagaaaccac ttcaacgtgg tgcgggacat
    15301 tcaggatttc gaagatttcg gcggcgaact gcatggtagc ccagtcgctt ttggacccca
    15361 tcacgatggc gacacgcgcc ggattattgc gggaagacat gcgtcttaaa actcctgtgg
    15421 tgcacaactc tcggctttag agggcacaga gaatagcacg gaaagagagc aaggaaaacg
    15481 gttgcgtggc tgtgaaatca gcaaagttgt gggtttttta aaacggaaaa tgaatcagct
    15541 caacgtcatc cgccgtgact ttcaccatcg aaccttccgt atgccaggca ccgagtacca
    15601 cgcgaaaagc aggttgctgg ttggcgataa gttcatgtac cgccgggcga tgggtatgtc
    15661 cgtggatcag ccattgcacc tgatgttttt ccatcgcact gactaccgcg ttttggttaa
    15721 cgtccatgat cgccagcgat ttactgctgt tggcttcttt actgttcgct cgcattcgcg
    15781 cggcaatgcg tttgcgcaca aacaacggca gggcgaggaa tagcatttgc agccagggtt
    15841 tgtggacttt ggcgcgaaaa gcctgataac ccgcgtcatc ggtgcacagc gtgtcgccat
    15901 gcataatcaa cacccggcga ccataaagtt cgagcacctt ttcttccggc aataatgtca
    15961 tgccgctttc acgggcaaag cgtttgccga gcagaaaatc acggttgcca tgaatgaaat
    16021 aacagggaac gccggaatcg gacaccgctt tgatcgctgc cgccatctgg cgatggagtg
    16081 ggttgggatc gtcgtcgcca atccatgctt caaacagatc gccaagaata tacagcgcgt
    16141 cggccttgcg ggcttccccc gctaaaaaac gcagaaaacc ggcggtgatc gccggttctt
    16201 ccacgcagag atgaagatct gcaataaaga gtgtcgccac gattactcgc taacggtcac
    16261 gctttcaatg ataacgtctt cttttggcac gtcctggtgc ataccgctac gaccggttgc
    16321 aacacctttg attttgtcta ccacgtccat gccttcaacc acttcagcaa acacgcagta
    16381 gccccaacct tgcaggcttt cgccagagaa gttcaggaag tcgttatcaa ccacgttgat
    16441 gaagaactgt gcagttgcag agtgcggagc ctgagtacgg gccattgcca gcgtaccacg
    16501 ggtatttttc agaccgttgt tggcttcgtt tttgatcggt tctttggtgg ctttttgttt
    16561 catgcccggt tcaaaaccgc cgccctgaat cataaagccg ttgataacac ggtggaaaat
    16621 ggtgttgttg taaaaacctt cgcggcagta gtccaggaag tttttaactg tttcaggtgc
    16681 tttatcgtca aaagttttga tgacaatatc gccgtgattg gtgtggaaag taaccatttt
    16741 tgcatcctgt tccgtttgat tggtgcttca acccagttcg ggtcatatat agggtggtgt
    16801 tatagcataa ccgcacgatc ggatcatcac gcaatgtatg ctgattcgcg cgggaaatat
    16861 gggtattata cgcaactcaa ttacccacac atgtctaaac ggaatcttcg atgctaaaaa
    16921 tcttcaatac tctgacacgc caaaaagagg aatttaagcc tattcacgcc ggggaagtcg
    16981 gcatgtatgt gtgtggaatc accgtttacg atctctgtca tatcggtcac gggcgtacct
    17041 ttgttgcttt tgacgtggtt gcgcgctatc tgcgtttcct cggctataaa ctgaagtatg
    17101 tgcgcaacat taccgatatc gacgacaaaa tcatcaaacg cgccaatgaa aatggcgaaa
    17161 gctttgtggc gctggtggat cgcatgatcg ccgaaatgca caaagatttt gatgctttga
    17221 acattctgcg cccggatatg gagccgcgcg cgacgcacca tatcgcagaa attattgaac
    17281 tcactgaaca actgatcgcc aaaggtcacg cttatgtggc ggacaacggc gacgtgatgt
    17341 tcgacgtccc gaccgatccg acatatggcg tgctgtcgcg tcaggatctc gaccagctgc
    17401 aggcaggtgc gcgcgttgac gtggtcgacg acaaacgcaa cccgatggac ttcgttctgt
    17461 ggaagatgtc gaaagagggc gaaccgagct ggccgtctcc gtggggcgcg ggccgtccag
    17521 gctggcacat tgaatgttcg gcaatgaact gcaagcagct gggtaaccac tttgatatcc
    17581 acggcggcgg ttcagacctg atgttcccgc accacgagaa cgaaatcgcg cagtccacct
    17641 gtgcccatga tggccagtat gtgaactact ggatgcactc ggggatggtg atggttgacc
    17701 gcgagaagat gtccaaatcg ctgggtaact tctttaccgt gcgcgatgtg ctgaaatact
    17761 acgacgcgga aaccgtgcgt tacttcctga tgtcgggcca ctatcgcagc cagttgaact
    17821 acagcgaaga gaacctgaag caggcgcgtg cggcgctgga gcgtctctac actgcgctgc
    17881 gcggcacaga taaaaccgtt gcgcctgccg gtggcgaagc gtttgaagcg cgctttattg
    17941 aagcgatgga cgacgatttc aacaccccgg aagcctattc cgtactgttt gatatggcgc
    18001 gtgaagtaaa ccgtctgaaa gcagaagata tggcagcggc gaatgcaatg gcatctcacc
    18061 tgcgtaaact ttccgctgta ttgggcctgc tggagcaaga accggaagcg ttcctgcaaa
    18121 gcggcgcgca ggcagacgac agcgaagtgg ctgagattga agcgttaatt caacagcgtc
    18181 tggatgcccg taaagcgaaa gactgggcgg cggcagatgc ggcgcgtgac cgtcttaacg
    18241 agatggggat cgtgctggaa gatggcccgc aagggaccac ctggcgtcgt aagtaattgc
    18301 gctgttgccg gatgcgagtt ttcgcatccg gttatcgcct gcgccaccac aacattccca
    18361 taagcaccat ccccggcaac cacacccaca tcaattcaga aattatcacc tgatgcccgt
    18421 acggcgtggt gtaacgagac aatgcaaacg gcgcgacttt aatcacctgc cagggagcga
    18481 aaaagcgttc atctgaccac ggccacagcc agccaacgcc cttaccgcca gtggttaccg
    18541 aatccagcaa gctgtgcgat agcaacgaga cggttaaaaa cagccagcag cgaatcagcc
    18601 cagccctgaa ccatcggcgt ccaatcagca cacataacag cgggacaaca aacgcaaaca
    18661 ccagcgaatg ggtaaaaccg cgatgaccaa aaacattgcc gtaagcaacg ccaaatttaa
    18721 acgacaatac gtcggcgtcg ggcagcatcg ccaggatgat tccggcaaat aacagacgcg
    18781 gagggatgac tttcgaaccc aaccctaaac caatgcatag gggaacggcg gcgtgcgtaa
    18841 taacggttgg catgatggtc gcttcggcaa aatgtcgatg ctatcagcat ggatgaacgg
    18901 ggcgtagagg gcaaaagtct gaaaagagaa ccggcctgtt gatacaggcc gggaaaggga
    18961 tcaggcaaca acctgtacgc tgtgacctgc aaaactcact gtctgaccgg cgacgatttt
    19021 gcagcgtttg cgcgtttcaa ccgcaccgtc gactttcacc tggccttcgg caatcgcgat
    19081 tttcgcctgc gcgccgcttt cgctccagcc ttccagtttc agcaagtcgc acagctcaac
    19141 gtgcggatgt ttacctaaag aaaatgtcgc catgttactc atcctgtgga tcatgatatt
    19201 caacgcacgc ctgtagcgtg ttttcaatca gcgtggcaac cgtcatcggg ccaacgccgc
    19261 cgggaacagg cgtaatgtat gaggcgcgtt tagccgcgtc ttcaaacacg acgtcgccca
    19321 caactttgcc attttccaga cggttgatgc cgacatcaat cacaattgcg ccttctttga
    19381 tccagtcacc gggaataaag cctggcttgc caacggcaac gatcaacaga tcggcatttt
    19441 ctacgtgatg acgcagattt ttagtgaagc ggtgagtcac ggtagtggtg caacctgcga
    19501 gcaacagttc catgctcatt gggcgaccga cgatattcga tgcgccaatc accacggcgt
    19561 tgaggccgaa ggtatcaatg ttgtaacgct caagcagcgt gacgataccg cgcggggtgc
    19621 agggacgcag acgcggcgcg cgctggcaca gacgaccgac gttgtaagga tggaaaccgt
    19681 ccacgtcttt gtccggatga atacgttcca gcactttgac gttatcaata cccgccggta
    19741 acggcagttg aaccagaatg ccatcgatgg tgttgtcggc attcagcgta tcgataagct
    19801 ccagcagctc cgcttcgctg gtggtttccg ggaggtcata agagcgggag acgaacccga
    19861 cttcttcaca agccttgcgt ttgcttgcga cataaatttg cgatgcaggg ttactaccca
    19921 ccagcacaac ggccagtcct ggtgcccgca gtccggctgc aatacgcgcc tgaacttttt
    19981 gagcaacttc agagcgcacc tgctgcgcaa tcgttttacc gtcaataatc tttgctgcca
    20041 tcagagagag gattccatct gttacgtagg tcgaagggga tgcgcctatt ttgtcagaag
    20101 cggggcgcgc tgtcaggttt cgtttcagat ttatcgcgtg aagcgacctc ttgcgaaggt
    20161 taggcgcacc gtcgctgagg tcgctgagat tgaaagcttc atttttcgtc catgatggcg
    20221 ttgtaaatct ggaactgatt tatttccttg tttaaggatt aagataattt aagaaatacc
    20281 tgacaatata aaa
//
LOCUS       DAPGWD010000068        19747 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPGWD010000068 DAPGWD010000000
VERSION     DAPGWD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.068"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1257
                     /gene="trkG"
                     /locus_tag="RHX88_002485"
     CDS             <1..1257
                     /gene="trkG"
                     /locus_tag="RHX88_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkG"
                     /protein_id="HDV2231160.1"
                     /translation="RTRDGFIIIVMFWILFSVISAFPLWFDSELNLTFIDALFEGVSG
                     ITTTGATVIDDVSSLPRAYLYYRSQLNFIGGLGVIVLAVAVLPLLGIGGAKLYQSEMP
                     GPFKDDKLTPRLADTSRTLWITYSLLGIACIVCYRLAGMPLFDAICHGISTVSLGGFS
                     THSESIGYFNNYLVELVAGSFSLLSAFNFTLWYIVISRKTIKPLIRDIELRFFLLIAL
                     GVIIVTSFQVWHIGMYDLHGSFIHSFFLASSMLTDNGLATQDYANWPTHTIVFLLLSS
                     FFGGCIGSTCGGIKSLRFLILFKQSKHEINQLSHPRALLSVNVGGKIVTDRVMRSVWS
                     FFFLYTLFTVFFILVLNGMGYDFLTSFATVAACINNMGLGFGATASSFGVLNDIAKYL
                     MCIAMILGRLEIYPVIILFSGFFWRS"
     gene            1395..2189
                     /locus_tag="RHX88_002486"
     CDS             1395..2189
                     /locus_tag="RHX88_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDV2231161.1"
                     /translation="MSEKLKIVYRPLQELSPYAYNARTHSTEQVAQLVESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEMLKMDSVPVIVLSGLTDEQKKAYRLADNRLPMNAGWD
                     EDLLRMELSDLINADFDVSLTGFIPTEIDELLTDVLPGTGNEEEPYTTKIDTPVYEPS
                     GGKPDISELYDDTKTQELISRIRSASLDPDIEKFLLCAAERHTVFNFSRIADYYAHAP
                     AEIQCLFEESALVIIDYQQAIENGFVRMTQRMVEIMHGGEEEEEYA"
     gene            2182..3114
                     /locus_tag="RHX88_002487"
     CDS             2182..3114
                     /locus_tag="RHX88_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231162.1"
                     /translation="MRDDFCAFILTHGRPDKVLTYRTLRRAGYTGKIFIVVDDEDKTR
                     HQYMAEFGEQVLVFSKADIASRFDEADNFGDRRSIFYARNACFDLAKLVGCKYFIQLD
                     DDYHEFQFRVDRNYDQAYFPIRKLDAILSEMLAYYESIPALSIAMSQGGDFLGDNGGH
                     ASWVKRKAMNSFICSVDRPFSFMGRINEDVNTYTNLGRCGELFMTIGAVQLGQKQTQK
                     NSGGMTELYLDSGTYVKSFYSVMYSPSCVKISLMGASHKRIHHQVTWNNAAVKILHEK
                     YRKKTPCISMGVTNDSVFESRVSGSVPDDCTTNR"
     gene            3050..3301
                     /locus_tag="RHX88_002488"
     CDS             3050..3301
                     /locus_tag="RHX88_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231163.1"
                     /translation="MIPYSKVESLAACRMTAQQIADVLDVDLNRLKENREAMTDFYAA
                     IRKGRAKGEAELRAALFKLARKGDAFALRELLRVDKNQD"
     gene            3305..4399
                     /locus_tag="RHX88_002489"
     CDS             3305..4399
                     /locus_tag="RHX88_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDV2231164.1"
                     /translation="MSRPDWGALQQEYIAEYTRSGISPVAWCEARGLNYATARRYIKK
                     TPKNAQKEMRKTAQKSARKKTAQTAQRQNGKSQKKKAVSDALLIEGDTEEISFCPDEF
                     GISDQQAKFAMLVAQGKKPTEAYRLAGYEGQGATANSNASRMLRNARVYRAISYFRNQ
                     YQKRYTADLDLLVSQLMAIVQADPNQLAQFRRVNCRYCWGENHLYQWRDIAEFDKAAA
                     QASRDGKPEPEYGGLGFVDNAIPNPDCPKCCGEGTGQLYMADTTLLDGDARQLYAGSK
                     LGKYGVEILLEDKAAARRELLRLLSAGGALCADKRLQELEIERRRIENQKLRKEIETV
                     EDNEHPQPVAININVVDARLRSDEDDLSDA"
     gene            4380..5681
                     /locus_tag="RHX88_002490"
     CDS             4380..5681
                     /locus_tag="RHX88_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDV2231165.1"
                     /translation="MISPTLNVPQARFLSMPHKFKAYIAGFGSGKTWVGCGGICKGIW
                     EHPGINQGYFAPTYPQIRDIFYPTVEEVAADWGLNVKINEGNKEVHFYYGRQYRGTTI
                     CRSMEKPQTIVGFKIGNALVDELDILPKEKARTAWRKIIARMRYKIDGLRNGIDVTTT
                     PEGFKFVYEQFVKAVREKTELASLYGLVQASTFDNEKNLPADYIPSLLESYPPELIKA
                     YLLGRFTNLTSGTVYHQFDRKLNNCEEVEQPGEPIYIGMDFNVGKMAGIVHVLRLGLP
                     CAVTEIINAYDTPDMIRLIKERFWLYDGHDYRKVREIYIYPDASGDSRKSSNASTTDI
                     AQLKQAGFNVVVNSSNPPVKDRVNSMNAMFCNANGERRYKVNVKRCPVYAESLEQQVW
                     DDKGEPDKKSGNDHPNDAGGYFIVKQFPIVKPTGRVTSLRI"
     gene            5684..7090
                     /locus_tag="RHX88_002491"
     CDS             5684..7090
                     /locus_tag="RHX88_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HDV2231166.1"
                     /translation="MADISTPNLDYNDMLEAWDINDALMGGTLEMRRQGENYLPKWPN
                     EDEDAYKKRLSVATLLPVYEESIKQNIGRIFAEPTVLSEETPEKIREYAENIDMEGSR
                     LDVWAQQFFSLAFQYGVAHALVDYPRTDMNEIRTKADENAAGGRPYVTMLNPRQVIGW
                     KSKVEKGKVVLTDLRIKEVIIIDGDDFGQKKVEQIRHIMPRRVEIYRRSEGTNGESVW
                     MLHESWNTSRDDIPLVTLYTKKTGFMRGTPPLLNLGLLNIKHWQSQSEQDNILHVARV
                     PLLVAYGLDRNEELTVGASTATIFEDRTKNGLEYVEHSGAAIESGETSLEKLENQMRH
                     AGAKLLRAENTSTKSVDQTNEERMQEHSPLYTMANSLEDALDNILQIMAEWSGESCGG
                     NVDVRTELDVSAQVFDSSSALAVQSLRQGGDIRQIDAVRVLQALKFIDQDSRPEDVID
                     ELKNQSVMLMEINDANRE"
     gene            7074..8186
                     /locus_tag="RHX88_002492"
     CDS             7074..8186
                     /locus_tag="RHX88_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231167.1"
                     /translation="MQTVNERLRDESIAHAVWISRYSTGVAARMVKILNDSDAELTAR
                     LLVALDSLDPGSFTVTRLESLLASVREVNRAAINSMFTSLSGELNELAIYEAGYQLSL
                     FDSLLPDFVADVHPLVGISSDALYAAAMARPFQGRLLSEWALDLEADRLRRITNTVRQ
                     GFLLGDTNEQIARKIRGHVSKGFQDGALQMSRANAASIAKTAVGHLAATARESFASAN
                     NDLIKGKQWLSTLDNRTTPQCRIRDRLKYTLDNKPVGHSVPYLHGPGKIHFCCRSTET
                     FILKSAKELGIDVRDISPAERASMDGVVAGDTTYREWFLRQPYTRQKQIVGETRAKLI
                     RDGGMSPDEFYTDKGEWLTLKQLRERDAQVFRKAGI"
     gene            8291..9055
                     /locus_tag="RHX88_002493"
     CDS             8291..9055
                     /locus_tag="RHX88_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231168.1"
                     /translation="MKLKTVEINGKQYAEIDTAGLPVYVHDDGKEIGFDAPLAIKKIT
                     ELNGEAKNHRLAKEAAEEKLAKFAAIEDPKKAIEALEMLSKIDQKKLIDAGQVDQVKA
                     EITKNFQQQLDEEKQRSQMLETQLYDSMIGGSFAGSKYIADKIAIPADLLQARFGQAF
                     KVEEGKIVAYDASGNKIYSRAKPGELAQFDEALEFLVENYPQKDYILKASGNNGGGSR
                     PTQHDVGQKTMKRSAFDALDVAGKQNALKDGITIVD"
     gene            9154..10293
                     /locus_tag="RHX88_002494"
     CDS             9154..10293
                     /locus_tag="RHX88_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 coat - protein 5 family protein"
                     /protein_id="HDV2231169.1"
                     /translation="MAGNTLTGLIPTIYTALDVVSREQVGFIPAVAKNAKADAAAKDQ
                     TVTAPVAPEAKTEDIVPGPSAPNTGDQNIGTVDVKITKSKMAPVKWNGEEQLALGPSG
                     TYNTILADQFKQAFRALANEVEADLGALYFGASRAVGTAGTTPFGVKDDLSDAALARQ
                     VLEDNGAPTTDLQMVLGSTAIANLRGKQSVLFKVNESGTEQLLREGVLGRLEGFNIHS
                     SAGVKRAPKVAATGYLVNGEKKEGDVLISIDTGSGSISAGQIVTFAGDPNQYVVAAAT
                     SNLITLAAPGLRQDLADDTAITVVGAFTANMAFDRNAFLLASRTPAMPEGGDNADDVM
                     NVTDPISGITFQIALYRQYRQVRYEVGLAWGVSSVKPAHGCLILG"
     gene            10336..10512
                     /locus_tag="RHX88_002495"
     CDS             10336..10512
                     /locus_tag="RHX88_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004121546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231170.1"
                     /translation="MAGLTKEQRAQREAAQKTQIELVVMVTDYQMFPGAPTIANVHPD
                     EVDNWKAMGWKTQE"
     gene            10516..10911
                     /locus_tag="RHX88_002496"
     CDS             10516..10911
                     /locus_tag="RHX88_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein singed"
                     /protein_id="HDV2231171.1"
                     /translation="MITYVTCDDVDNAFGNAWTSENAKNKAVLMANAWLNGFNLKINP
                     SRIPEEVKLAGAYAARIASFGKLFQQKNDSGVVISKAVSADGVSVSKSFSELPANSTA
                     LLEPDLQLAIALLKPYGLSRSQVRVVRGG"
     gene            10911..11294
                     /locus_tag="RHX88_002497"
     CDS             10911..11294
                     /locus_tag="RHX88_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDV2231172.1"
                     /translation="MGLREEIQSEVAAAFDEDLADAVSDFSGSYVTHRHWDPVTETGG
                     ESTAIYTGRGVLTRYKLGRIDGINILHGDLKLTALVCEVTDKPAVDHIIEIYDPVSRQ
                     LQRYEVITASVDPSASVYSIQLRRA"
     gene            11295..11675
                     /locus_tag="RHX88_002498"
     CDS             11295..11675
                     /locus_tag="RHX88_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDV2231173.1"
                     /translation="MAKAWDIEPSIFAGMIEEDVGLKIRYIAIQILTAIDIAAPVDTG
                     RFRNNNLVSLQHPDFGISDNVDPNGTIAVQRGIGVISKAANYGVIYIQNNLPYAEALE
                     NGHSQQAPTGVYANAFHGVLQAYK"
     gene            11672..12064
                     /locus_tag="RHX88_002499"
     CDS             11672..12064
                     /locus_tag="RHX88_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HDV2231174.1"
                     /translation="MKFTEIRNTVISRMTAQTIIDGKDVLYPNGPTFDPSGKLIWARL
                     SNIPGQAGVNEIGAGPVVYRTGIIIIQLFVPAGSGSKLITETADKLRELFEFQDDDRL
                     SYQAVSSIAVGEKNGWFQLNLQIPYRAL"
     gene            12091..13053
                     /locus_tag="RHX88_002500"
     CDS             12091..13053
                     /locus_tag="RHX88_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231175.1"
                     /translation="MSSGAKVISAFIRETVAGTTPASGDWSLLKRTSWGVKPTQNKGE
                     NNEIGGSRMAQGATPGTVDVGGDVGTKFRWGQHDDFLASCFGAEWSGDSLTMGNERIT
                     FSLATYASDVGIASVVRGAQVGSWKMQIPNDGDITATVTFAGLDWESKADDTNFIKGE
                     PVDSAGKLRYSFKEVSAVSLNGVAGGNGFCIDSFDIQFDNKLQTQRCIGTGSPYAGAN
                     IPTTFTPSGTVTLSWSKAAWEIWSKTLTGETVPFSFTLSNGEGAYTFSFPKVQVSGEW
                     PDGGNSDIIQVQLSITAADEAPTITRKKNSPAAVIAKASAEAIS"
     gene            13204..13563
                     /locus_tag="RHX88_002501"
     CDS             13204..13563
                     /locus_tag="RHX88_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231176.1"
                     /translation="MVASSDNDQYRSRNALIRRHIEKMDASLHVGTKEFDISKVSEVD
                     SVDDLLIDNAARYLLKDWKGVGELVNGVEVALEYTAERGIALLKQNPELYWQILAEAA
                     SIAQGKEQQKQDTIKKP"
     gene            14035..17268
                     /locus_tag="RHX88_002502"
     CDS             14035..17268
                     /locus_tag="RHX88_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV2231177.1"
                     /translation="MAEQTSRLAIIIDSTGAKNNADNLTSSLVKMTQAGETAANSAGK
                     VTKATEDEKNALAKLKAAIDPVGAAIDTVGRRYSELKKFFDKGLIDKEEYEFLVRKLN
                     ETTEELSGVAQAQREAEKAGKLAAAQQEAQAQAFQRMLDKIDPLAAALRNLEQQHDEL
                     NAAFASGKINGSQFENYSRKIQETRRELTGEAQAEREAAKAHDEQVVALQRLIAQLDP
                     VGTAFNRLVEQQKQLNEAKAKGMLSPEMYEELSGKLCAMRSELEVTQSQLSKTGMSAK
                     QTAFAMRMLPAQMTDIVVGLSTGQSPFMVLMQQGGQLKDMFGGIGPAIKGVGSYVLGL
                     INPFTLAAAAVGVLGLAYYKGSQEQDEFNKSLILTGNQLGTTSGQLGDIAQRAGNAAD
                     STTGAAAAVLNQLVRSGKVASSSLEQVTTAIVKTSEVTGISTEQLVNDFNEIAKDPVS
                     AISKLNDQYHFLTLATYNQIKALQDEGNQQEAARIATEEYSSSMIQRTNQIKENLGYL
                     ETAWKAVADSAKWAWDSMLDIGREASLDQKISDVLRQIDEIEKNTRPGVFGLGGIGDG
                     GAQNKRLARLKQQLGVLQAEKIAQDVLNSSINDYNKRQQEGIELRQRADAFSKQYQTR
                     EQQRASELAKLEKLKNQYSKEEYNNLIAQINERYKDPKQPKAKGYSDDAAQRMIDHLN
                     QQNALLSSQAELTVKLSSSEQELVKWRQRIADLESRPSSKLTQDQKSLLLHREEITTL
                     MEKNVAIEKNNRLIKESAEITAWRDSLQASIDNRQQGYDIQIAGYGVGDKNQQRQQEL
                     LRIEHGYNNQRLQLERDYADKSRGMSDHVFQEKMQALNDALEREKEIVRQKNEQLDIQ
                     AGDWVSGASQGFNNWLDDTKDISEQIKSTTTQMFDGMTDALGDFVTTGKANFRSFATS
                     VISDLSRIALKASITGIFDSISNSSSGGILGTIGSAISKFIPNAKGGVYESPSLSTYS
                     NGIYDSPQFFAFAKGAGVFGEAGPEAIMPLTRTSDGSLGVRAINSKSGNGGGDITYAP
                     VYQITIQNDGQNGEIGPQAIKALMGMVDQRVQGNLLNMRRDGGMLSG"
     gene            17261..17599
                     /locus_tag="RHX88_002503"
     CDS             17261..17599
                     /locus_tag="RHX88_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231178.1"
                     /translation="MANTEEFHWLPEDGMKTENKPSIKTVRFGDGYEQRSPNGLNHSL
                     RVFTCDFKVEANERDSFEKFLARHEGYKSFFWRPPGINRKIRVVCRTWSATEHITYTD
                     FSCQFDEVVI"
     gene            17599..18297
                     /locus_tag="RHX88_002504"
     CDS             17599..18297
                     /locus_tag="RHX88_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV2231179.1"
                     /translation="MQDIQQETLNECIRAEQSASVVLWEIDLTEVGGDRYFFCNEQNE
                     KGESVTWQSRQYQAYPIQGSGFEMNGKGSAARPTLTVSNLHGMITGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSEADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGSFLSINKLSQ"
     gene            18303..19046
                     /locus_tag="RHX88_002505"
     CDS             18303..19046
                     /locus_tag="RHX88_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231180.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEEERYFPCVNISGEPE
                     EYFRMSPEDWLQAKMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            18983..19585
                     /locus_tag="RHX88_002506"
     CDS             18983..19585
                     /locus_tag="RHX88_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV2231181.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGSEAIRALAMQIPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLENGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKPKTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
BASE COUNT         5304 a         4083 c         5335 g         5025 t
ORIGIN      
        1 cgtacccgtg atgggtttat tataattgta atgttttgga ttttgttttc tgttattagt
       61 gcattccctt tatggtttga ctcagaactt aatttaacgt tcattgatgc tctgtttgaa
      121 ggggtttctg gaataacaac aacaggagca actgtaattg atgatgttag ttcattacct
      181 cgggcatatt tgtactatcg gtcacagtta aattttatag gtggtttagg agttattgtt
      241 ctggcggttg cagtattgcc attattgggt attggtggtg caaagcttta tcagtcagaa
      301 atgccggggc catttaagga tgacaaactc actccccgcc tggccgatac gtcacggaca
      361 ctgtggataa cttattcttt attaggtatt gcttgtattg tctgttatag acttgcagga
      421 atgcctttgt ttgatgctat ttgtcacggg atttccacag tttcgcttgg tggtttctca
      481 actcatagcg agagtatcgg atattttaat aactatttgg ttgagctggt ggctggttct
      541 ttttccctgc tatcggcttt caacttcact ctttggtata ttgttattag cagaaaaacg
      601 ataaaacctt taatcagaga tattgaactt cgtttctttc tgttaatagc cttaggggtg
      661 atcattgtta cctctttcca ggtctggcat ataggtatgt atgacttgca tggaagtttt
      721 attcattcgt tttttcttgc cagctccatg ctcactgata atggtttagc tacgcaggat
      781 tatgcaaact ggcccacgca cacgatagtg tttttgctgt tgtcaagttt ctttggggga
      841 tgtataggtt caacctgtgg tggaattaag tcacttcgat ttcttatact tttcaaacaa
      901 agcaaacacg agataaatca gctttctcat cccagagcgt tgttgagtgt aaatgtagga
      961 gggaagatag ttacagatcg tgtaatgagg tctgtatgga gtttcttttt tctttatact
     1021 ctcttcacgg tgttttttat actggtgtta aatggtatgg gatatgattt tcttacatca
     1081 tttgcaacag tggctgcatg tattaataat atgggattag gttttggggc tactgcatcg
     1141 tcattcggag tgcttaatga cattgcaaaa tatttaatgt gcatagctat gattcttggt
     1201 cgccttgaaa tttatcctgt tattatattg ttttcaggtt ttttttggcg ctcctaatat
     1261 atggctgatt tataattgtg agtttaatat tatgttgact cactcattga tccaatacct
     1321 aactttacca gcaacacctc cgcccccagt agcactggct gctggggtgc gttttattca
     1381 taaagcaagg ctgtatgagc gagaaattaa agatagtcta tcgcccatta caagaattgt
     1441 caccgtatgc gtacaacgcc aggacgcaca gtactgagca ggtggcacaa ctggtagaaa
     1501 gtattaagca attcggctgg actaatccgg tgctgattga cgaaaagggc gaaattattg
     1561 cgggtcacgg tcgtgttatg gcggctgaaa tgctcaaaat ggattctgtt ccggtcattg
     1621 ttctgtctgg cctgacggat gagcagaaaa aggcgtaccg cctggcagat aatcgcctgc
     1681 cgatgaatgc tggctgggat gaagatctgt tgcggatgga gctgtcggac ctaatcaatg
     1741 ctgattttga tgtctccctg acaggcttca tcccgacaga aattgatgaa ctgttgacgg
     1801 atgttttgcc cggtacagga aatgaggagg agccgtatac gacgaaaatt gatacgcctg
     1861 tttatgagcc gtcgggcggt aaaccggata tcagtgaact gtacgacgat acgaaaactc
     1921 aggagctgat cagccggata cgttcggcgt cccttgatcc tgatattgag aaattcctcc
     1981 tgtgtgcggc agaacgtcac acagtgttta atttcagcag aattgcggac tattacgctc
     2041 atgcccccgc tgaaattcag tgccttttcg aggagtcggc gctggtgatc attgattatc
     2101 agcaggctat tgaaaatgga tttgtccgaa tgacgcagcg catggtggag atcatgcatg
     2161 gcggggagga ggaggaggaa tatgcgtgat gatttttgcg cctttattct gactcacggg
     2221 cgaccggaca aagttctgac ttaccggacg ttgcgtcgtg ctggctatac cgggaaaatt
     2281 tttatcgttg ttgatgatga agataagaca cggcatcagt acatggctga atttggtgaa
     2341 caggtgctgg tattttccaa agccgatatt gccagtcgtt ttgacgaagc cgataatttt
     2401 ggtgatcgcc gctcaatttt ttacgcccgt aatgcctgtt tcgacctggc aaaactggtc
     2461 gggtgtaaat acttcattca gctcgatgat gattatcacg agttccagtt tcgggtggat
     2521 cgcaactatg accaggccta tttcccgata aggaaactgg atgcgatcct ttctgaaatg
     2581 ctggcgtatt acgaatcaat acccgcgctt tccatcgcta tgtcgcaggg cggggatttt
     2641 cttggtgaca atggcggcca tgcttcgtgg gtgaaacgca aggcaatgaa cagctttatc
     2701 tgttcggttg atcgaccgtt ctcattcatg gggcgcatta acgaggatgt gaatacgtac
     2761 acgaatctcg gtcgctgtgg tgaattgttt atgacgatcg gtgctgtcca gttagggcag
     2821 aaacagacgc agaaaaacag cggcggaatg accgagctgt atctggattc cggaacctac
     2881 gttaaaagtt tttactccgt catgtattcg ccgtcgtgcg taaaaatctc actgatgggt
     2941 gccagccata aacgcattca ccatcaggtc acctggaaca acgctgcagt aaaaatcctt
     3001 cacgaaaaat acaggaagaa gacaccctgc atatcaatgg gggtgacaaa tgattccgta
     3061 ttcgaaagtc gagtctctgg cagcgtgccg gatgactgca caacaaatcg ctgacgttct
     3121 ggatgttgat ctgaaccgac tgaaagaaaa tcgggaagca atgacagatt tttatgcggc
     3181 catccgtaag ggcagagcga aaggtgaagc cgagttacga gcggcattgt ttaagcttgc
     3241 cagaaaaggg gatgcctttg ctctgcgcga actactcagg gtggataaaa atcaggacta
     3301 actgatgagc agaccggact ggggggcgtt gcagcaggag tatattgctg aatacacccg
     3361 ctccggtata tctccggtgg catggtgtga agcaagggga ctgaactacg caacagcccg
     3421 tcgttacatc aaaaaaactc cgaaaaatgc gcagaaagaa atgcgcaaaa ctgcgcaaaa
     3481 aagtgcgcga aaaaaaactg cgcagactgc gcaaaggcag aacggaaaat ctcagaaaaa
     3541 aaaggctgta tccgatgcac tcctgattga gggggacacg gaagaaattt cgttctgccc
     3601 cgatgaattc ggtatttctg accagcaggc aaaattcgcc atgttggttg ctcaggggaa
     3661 aaagccgaca gaggcatacc gactggctgg ttatgagggg caaggtgcga cagctaacag
     3721 caacgccagc cgtatgctta gaaatgccag ggtttatcgt gctatcagct acttccgcaa
     3781 tcagtaccag aaacgctata ccgcagacct ggatttactg gtgagccagt tgatggccat
     3841 tgtccaggcc gaccccaatc agttggcaca atttcgccgt gttaactgcc gttattgctg
     3901 gggcgagaat catctctacc agtggcgtga tattgcagaa ttcgataagg ctgcggcaca
     3961 ggcctccaga gatggtaaac ccgagccgga atatggaggc ctcggctttg ttgataacgc
     4021 catacccaat cctgattgcc cgaagtgctg cggtgaggga acaggacagc tttatatggc
     4081 tgataccaca ctgcttgatg gggatgcgcg gcaattatat gcagggtcaa agctcggaaa
     4141 atacggtgtt gagatcctgc tggaggataa ggctgccgcc cggcgtgaat tgttgcgttt
     4201 gctttctgct ggcggggcat tatgtgcaga taagcggcta caggaactgg aaattgaacg
     4261 gcgcagaata gagaaccaga agctgcgcaa agagatcgaa acggtggagg ataatgaaca
     4321 tccccagcct gtggcgatca atattaatgt ggttgatgcc agattaagga gtgatgaaga
     4381 tgatctctcc gacgcttaat gtgcctcagg cgcgatttct ttcaatgccc cataaattta
     4441 aagcctatat tgctggtttt ggctcgggca aaacatgggt tgggtgtggc ggcatatgca
     4501 aggggatttg ggagcatcca ggtataaatc agggatattt tgcgccaacg tatccccaaa
     4561 ttcgcgatat tttttaccct acagtggaag aagttgctgc tgactgggga ttgaacgtaa
     4621 aaattaatga gggaaataaa gaggttcact tttattacgg acgccagtat cggggaacca
     4681 ctatctgcag atcgatggag aagccacaaa cgatcgtcgg tttcaaaatt ggtaatgcgc
     4741 tggtggatga actggacatt ttgccgaagg aaaaagccag aacggcgtgg cgcaagataa
     4801 ttgcgcgtat gcgttataag attgatggac ttcgcaacgg tattgacgtt acaaccacgc
     4861 cggaaggatt caaatttgtc tacgagcagt ttgttaaagc cgtgcgtgaa aaaacagagc
     4921 tggcctcact gtatggtctg gtgcaggcat ctactttcga taatgaaaag aacctgccag
     4981 cagattacat tccttcactt cttgaatcat accctccaga gctgattaaa gcctatcttc
     5041 taggacggtt tactaacctg acaagtggta ctgtttacca tcagtttgac cggaaactga
     5101 ataattgcga agaagtggag cagccagggg agccgattta tattgggatg gattttaacg
     5161 ttggaaagat ggcgggaatc gtccatgtgc tgcgtttggg gcttccatgt gcggtaactg
     5221 aaatcatcaa tgcctacgat acgccggata tgatccgcct cattaaagaa cgcttctggc
     5281 tgtatgacgg gcatgattac cggaaagtga gggagattta tatttatcca gatgcttccg
     5341 gagattccag aaaatcaagt aacgcaagta cgacggatat agcccagctt aagcaggcag
     5401 gttttaacgt tgtggtgaac agctcgaacc cgccagtaaa agatcgcgtt aactcaatga
     5461 atgcaatgtt ctgcaatgcc aatggtgagc gtcgctataa agttaatgtg aagcgttgtc
     5521 cggtatatgc cgaatctctg gagcaacagg tctgggatga taagggggag cctgataaaa
     5581 aatctggcaa tgatcacccg aatgatgccg gaggttattt catcgttaag caattcccta
     5641 ttgtcaaacc gaccggaaga gtcacatcac ttcggattta attatggctg atatatcaac
     5701 acccaacctc gactataacg atatgctgga ggcgtgggat attaacgacg cattgatggg
     5761 cggtacgctt gaaatgcgca ggcaggggga aaattatctc cccaaatggc ctaatgaaga
     5821 tgaagacgct tataaaaaac gcctgtctgt ggctacgtta cttcctgtgt atgaagaaag
     5881 catcaaacaa aatattgggc gcatatttgc agagccgaca gtattgagtg aggaaacgcc
     5941 ggaaaaaatc agggaatatg cagaaaatat cgacatggag gggagccgac tggatgtgtg
     6001 ggcgcagcaa tttttcagtc tcgcatttca gtatggtgtg gcacatgcgc tggtggacta
     6061 tccacgaacg gatatgaacg agattcggac aaaagccgat gaaaacgcgg ccggtggtcg
     6121 cccgtacgtt acgatgctga atccacgcca ggttattgga tggaaatcga aagttgaaaa
     6181 agggaaagtt gttctcactg atttgcgtat aaaagaggtc atcattattg atggtgatga
     6241 tttcgggcag aagaaggtgg agcagatccg ccatattatg ccccgtcgag tagaaattta
     6301 tcgacgcagc gaaggtacta atggcgaatc tgtctggatg cttcatgagt catggaatac
     6361 cagccgtgat gatattcctc tggtaacact gtatacgaag aaaacagggt ttatgcgtgg
     6421 tacaccgcca ttgcttaatc ttggcttgct gaatatcaag cactggcaaa gccagagcga
     6481 gcaggacaat attcttcatg ttgcccgcgt tccattgctg gtggcctacg ggctggacag
     6541 gaatgaagaa ctgacggttg gtgcatccac ggctacgatt tttgaggaca gaacaaaaaa
     6601 tggcctcgaa tatgttgaac atagtggcgc agcgatagaa tctggcgaaa catcacttga
     6661 gaagctggaa aatcagatgc gtcatgccgg cgctaaactt ctgcgggctg aaaatacatc
     6721 caccaaatct gttgatcaga ctaatgaaga gcggatgcag gaacactcgc cgctttatac
     6781 aatggcgaac tccctcgaag atgccctcga taatattctc cagataatgg cggaatggag
     6841 cggagagagt tgcggtggca atgtggatgt gcgcactgaa ctggatgtat ctgcccaggt
     6901 gtttgactca tcctccgcgc tggctgttca gtcattgcgt cagggcggtg atatacgtca
     6961 gattgatgcg gttcgggtgt tgcaggcgtt gaaatttatc gatcaggatt cccgtccaga
     7021 ggatgtgatc gatgaattaa aaaatcagag tgtaatgttg atggaaatca atgatgcaaa
     7081 ccgtgaatga acggctgcgt gatgaatcaa ttgctcatgc agtctggata tcccgctata
     7141 gcactggtgt ggctgccaga atggtgaaaa tactgaatga cagcgatgcg gagcttacag
     7201 ctcgcctgct ggtagcactg gacagcctcg atcctggtag ttttaccgtt acgcgcctag
     7261 agtcacttct ggcgagtgtc agagaggtta accgcgctgc tattaacagc atgtttacca
     7321 gtctctccgg agagctgaac gagctggcaa tttatgaggc tggttatcag ttaagtctgt
     7381 ttgattctct gctacctgat tttgttgctg atgttcaccc tctggttggt atatcttctg
     7441 atgcacttta cgccgctgca atggcgcgac cattccaggg acgactgctc agtgagtggg
     7501 ccttggatct tgaggcggat cggctcagac gcataacaaa tacggtgcgt cagggttttt
     7561 tgctggggga taccaatgag cagatcgcaa gaaaaattcg gggacatgtc agcaagggat
     7621 ttcaggatgg tgcattgcag atgagccggg ctaatgcggc cagcattgca aaaacagcgg
     7681 ttggacatct tgctgctact gcccgtgaga gttttgccag cgcgaataat gatttgatta
     7741 agggtaagca atggttatca acgcttgata atcgtactac gccacaatgt cgaatcaggg
     7801 atcgcctcaa atatacgctg gataataaac ctgtaggtca cagtgtgcct tatttgcatg
     7861 ggccgggaaa aattcatttc tgctgccgct caacggaaac gttcattctg aaatcagcga
     7921 aggaactggg tattgatgtt cgtgatattt ccccggctga gcgggccagc atggatggcg
     7981 tggtggccgg agatacaacc tacagggaat ggtttttgcg tcagccttac accagacaaa
     8041 aacagattgt gggggaaacc cgggcaaagc tgattcggga tggcggtatg tcgccagatg
     8101 aattttacac cgataaaggc gaatggctga cgctgaagca actccgtgag cgtgatgcac
     8161 aggtattcag aaaagcaggg atttaaataa atcatttatt acaacaggct accttcgggt
     8221 ggcctttttt attgctgcga tccggatggt gagcagcgta actgtcggaa gacttaaacc
     8281 aggtactaat atgaaactga aaacggtcga gattaacgga aaacaatacg cagaaattga
     8341 tactgctggc ctgccagttt atgtgcacga cgatggtaaa gaaatcggct tcgatgcacc
     8401 gctggcgata aaaaaaatta cagagcttaa tggcgaggca aaaaatcatc gcctggctaa
     8461 agaagctgca gaggaaaaac tggctaagtt tgccgctatc gaagacccga agaaggcgat
     8521 cgaggcactg gaaatgctgt caaaaatcga ccagaaaaag ctgatcgatg cgggacaggt
     8581 tgaccaggtt aaggcagaaa ttacgaaaaa ttttcagcag caattagatg aagaaaagca
     8641 acgctctcag atgctggaga cgcagcttta cgattctatg attggcggta gttttgcggg
     8701 ttcaaaatat attgccgata aaattgcgat cccggcagat ttattacaag cccgcttcgg
     8761 gcaggcattc aaagtggaag aagggaagat cgttgcttat gacgcttccg gcaacaaaat
     8821 ttattcccgc gcgaagcctg gcgaactggc gcagtttgat gaggcgctgg agttcctcgt
     8881 cgaaaattac cctcagaaag actacatcct gaaagccagt ggcaacaatg gcggcggctc
     8941 ccgtccgaca cagcatgatg ttggtcagaa aacgatgaaa cgctctgctt ttgatgcact
     9001 ggatgttgca ggtaagcaaa acgcattgaa agacggtatc acaattgttg attaacacat
     9061 ttgtcagctt ccggatggga gctggtgtca gggttggata gctcattact ccatctattc
     9121 acaattacgc aaatttttaa ggaatattta attatggctg gaaataccct gaccgggttg
     9181 atcccgacta tttataccgc cctggatgtt gtatcccgtg agcaggtagg ttttatccct
     9241 gcggtagcaa aaaacgcaaa agctgacgcc gcagcaaaag atcagacggt aaccgcgcca
     9301 gttgcgcctg aggcgaaaac cgaagatatc gtaccggggc cgtcagctcc gaataccggt
     9361 gatcaaaata ttggcactgt tgatgtaaaa attactaaat ccaaaatggc accggttaaa
     9421 tggaatggtg aagaacaact ggctcttggc ccttcaggga cttacaacac cattctggct
     9481 gatcagttca agcaggcttt tcgcgccctt gcaaacgagg ttgaggctga tcttggtgcg
     9541 ttgtatttcg gtgcttcccg cgccgtggga accgcaggga caacgccgtt tggtgttaaa
     9601 gatgatcttt ctgatgctgc tctggctcgt caggttctgg aggataacgg cgcaccgaca
     9661 accgatctgc agatggtgct tggctccact gccattgcta atttgcgcgg aaaacagtct
     9721 gtattgttta aagtgaatga atccggcact gaacagctac tgcgtgaggg cgtgttgggg
     9781 cgtctggagg gattcaatat tcacagttcg gcaggtgtga aacgagcgcc aaaggttgct
     9841 gcaactggtt atctcgtgaa tggagagaaa aaagaaggcg atgttcttat ttccattgat
     9901 actggttcgg ggagtatttc tgcaggtcag attgttacgt tcgctggtga tccgaatcag
     9961 tatgttgtgg cagcagcgac cagcaatctg attactcttg cagcgcccgg actgcgtcag
    10021 gatctggccg atgatactgc catcacagtt gttggggcct ttactgcaaa tatggcgttt
    10081 gatcgcaacg cgtttctgct ggcatcccgt actccggcaa tgccggaagg tggcgataat
    10141 gctgatgatg taatgaatgt tacggacccg atttcaggga ttacgttcca gattgcactg
    10201 tatcgtcagt atcgccaggt gcgctatgaa gttggactgg catggggtgt ttcatcagtg
    10261 aagccggcac atggttgtct gattcttggt taaacattca aacggggctt cggccccatt
    10321 tttaatgggg ggcatatggc cggattaact aaagagcaaa gagcacagcg tgaagccgca
    10381 caaaaaacgc aaattgagtt agtggttatg gtgaccgatt atcagatgtt tcccggcgca
    10441 ccaactatcg ctaatgttca tcctgatgaa gttgacaact ggaaggctat gggctggaaa
    10501 actcaggagt aacacatgat cacttacgtg acctgtgatg acgttgataa tgcgtttggg
    10561 aacgcctgga cgagtgagaa cgctaaaaat aaagctgttt taatggctaa tgcctggctt
    10621 aatggcttca acctgaaaat taacccatcc cgtattcctg aagaggtaaa acttgcggga
    10681 gcatatgcag ccagaattgc ctcattcggt aagttgtttc agcagaaaaa tgattctggc
    10741 gttgttatca gtaaagccgt gtcggctgac ggggtcagtg tatcgaaatc attttctgag
    10801 ttgccagcaa acagcactgc attgcttgaa cccgatttac agcttgcgat agcactgctg
    10861 aaaccgtacg gacttagtcg ctcacaagtc agggttgtga gggggggata atggggcttc
    10921 gtgaagagat tcagtcagag gttgccgctg cttttgatga ggatttagcg gacgccgtga
    10981 gtgatttttc tggttcttac gttacgcacc ggcactggga tcctgtgacg gaaactggcg
    11041 gcgaatccac cgcaatctat accgggcgag gcgtgttgac acgttataag ctgggcagaa
    11101 ttgatgggat taatattttg catggtgacc ttaaattaac cgctctggta tgcgaggtta
    11161 cggataaacc agctgtcgac catattattg agatttatga tcctgtatca aggcagttac
    11221 aacgatacga ggtaattaca gcaagtgtag atccttccgc atcagtttac tctatccagt
    11281 taaggagagc gtaaatggca aaggcatggg atatagaacc gtcaatattt gccgggatga
    11341 ttgaggaaga tgtagggctg aagattcgct acatcgctat tcagattctt actgctattg
    11401 atattgctgc tccggttgat accggacgtt tcagaaataa caacctggtg tcgttacagc
    11461 atcccgattt tggtatatct gataacgtgg atccgaacgg tacgattgct gttcagcgtg
    11521 ggatcggggt tatttcgaaa gctgcaaatt acggggttat ttatatccag aataaccttc
    11581 cttatgcaga ggctcttgaa aacggtcatt cacaacaagc gccaactggc gtgtatgcca
    11641 acgcttttca tggtgtttta caggcttaca aatgaagttt actgaaatca gaaatacggt
    11701 tatttccaga atgacggcac agacgattat tgatggaaaa gacgtattgt atccgaacgg
    11761 gccaacgttc gatccttccg gtaagttaat ctgggcgcgg ttaagtaata ttcccgggca
    11821 ggctggagtt aatgaaattg gcgcggggcc ggttgtttat cgcacgggga taatcattat
    11881 tcagttattt gtccccgcag gttctggttc aaaactcatt actgagacag ccgataaatt
    11941 gcgggaactg tttgagtttc aggatgacga tcgtctcagt taccaggctg tttcctcaat
    12001 agccgttggc gaaaagaatg gctggttcca gcttaatctt caaattccat atcgcgcgct
    12061 ctagcgcaat taatgacata ggaggctcct gtgagttcag gtgcaaaggt tatctcggca
    12121 tttatccggg agacggttgc aggcactacg ccagcaagtg gtgactggag tttattaaaa
    12181 cgcacaagct ggggagtaaa acccacccag aataaaggcg aaaataatga gattggtggc
    12241 tcccggatgg ctcagggggc gacgcctggc actgtggatg tcggcggtga tgttggtacc
    12301 aaatttcgct ggggtcaaca tgatgatttt cttgcatcct gtttcggcgc ggaatggtca
    12361 ggcgattctc tgacaatggg gaatgagcga ataacatttt ctctagcgac ctatgcgtcc
    12421 gatgtcggaa ttgcctctgt cgtcagagga gcgcaggttg gctcatggaa aatgcagatc
    12481 cctaacgacg gcgatattac agcgaccgta acctttgccg gactggactg ggaatcaaag
    12541 gccgatgata cgaattttat caaaggcgaa cctgtggata gtgcaggaaa gctacgttat
    12601 tcgtttaagg aggtttcagc agtaagcctg aatggtgttg ccggaggtaa cggtttttgt
    12661 atcgacagtt ttgatattca gttcgataac aaactccaga cacagcgttg tatcgggact
    12721 ggctcgcctt atgcaggagc aaatattccg actactttta caccgtccgg tacggtgacg
    12781 ctttcatggt ctaaagccgc gtgggaaatc tggagtaaaa cactgactgg agaaacagtt
    12841 ccgttcagct tcacgctttc gaatggagag ggtgcataca ctttcagttt cccgaaggtt
    12901 caggtgtcag gtgaatggcc tgatgggggt aatagcgaca ttatccaggt tcaattgagc
    12961 attaccgcag cagatgaagc acctacgata accagaaaaa aaaattcccc ggctgccgtg
    13021 atcgcaaaag ccagtgctga ggcgattagt tgattttccg ttattcctcc tgtggtactg
    13081 ccctacaggg ggacgcattg aatgaggtta tggatgttta ttcttaatca gaaaattatc
    13141 attggtggag aacgctggtt tacgccaatg aaggacttaa aacctgtaga cgggttaaaa
    13201 ctgttggtgg caagcagcga taacgatcag tatcgctccc gtaatgcatt aatccgtcgc
    13261 cacattgaga aaatggatgc cagtttgcac gtcggaacga aggagtttga tatttcaaag
    13321 gtttccgagg tggattctgt tgatgattta ctcattgata atgccgctcg ttatctgctg
    13381 aaagactgga aaggggttgg tgaactggtt aatggtgttg aggttgcact ggaatatacg
    13441 gcagaacgag ggatcgcgct gcttaagcag aatccagagt tgtactggca gatccttgca
    13501 gaagcagcca gcatcgccca gggtaaagag cagcagaagc aggatacgat aaaaaagcca
    13561 tagctgccca gcggtggtta tcggagttcg ggggagaaag gggggaaaag gcaagatgga
    13621 agcgagaaaa actcaggttg ccaccgatac cggaaccaga aatagacccg gtgcttaagg
    13681 agttgttgta cgcctattcg gtaatatccc gtgcccgacg ttatgctgga atggctgggg
    13741 tgcctttgcc tttatctctg acagagataa atgaatattt agccactcat ccggtattga
    13801 ttgagcgcga tgaatttgaa gcagtgatct ttgcactgga tgaccagtat tttcaggagc
    13861 agtgtgtagt tgttaattac gtacactctg ttacagagat gtgatggtgt ctttaattaa
    13921 atcgatgatg ctccttgaga aaagcattgc gtggcctcgt aatcgctata tctactatta
    13981 tgtcgcctga aacccacttc gcggtgggtt ttttgttgtc aggagtttta ataaatggca
    14041 gagcaaacct cgcgtctcgc aataattatt gatagcactg gagcgaaaaa taatgctgac
    14101 aatctgacct cctcattagt caaaatgacg caggctgggg aaactgctgc aaatagcgca
    14161 gggaaagtga ctaaggcaac agaagatgag aagaacgcgc tcgcaaaatt aaaagcagct
    14221 attgatccag ttggtgccgc aattgatact gtcggtcgac gctattctga attaaagaaa
    14281 tttttcgata aagggcttat tgataaagaa gaatatgaat ttcttgtccg taaacttaat
    14341 gaaaccacag aggaattgag cggggttgcg caagcgcaga gagaagccga gaaggccgga
    14401 aaacttgctg ccgcccagca ggaagcgcag gctcaggcct ttcaaagaat gctggacaag
    14461 atcgaccctc tggctgcggc gctaagaaat cttgaacaac agcatgatga acttaatgct
    14521 gcgtttgcat ccgggaaaat aaatggttct cagtttgaga attatagccg aaaaatacag
    14581 gaaacacggc gagagcttac tggagaggct caggcagagc gagaagcagc aaaagcgcat
    14641 gatgaacagg ttgttgcttt gcaacgtctg attgctcaac ttgatcctgt cggaactgct
    14701 tttaatcgtc tggtagaaca acagaaacag ctcaatgaag caaaagctaa ggggatgctt
    14761 tctcctgaaa tgtatgagga gctttctgga aaactttgtg ctatgcggag tgagcttgag
    14821 gttactcaat cacaattaag caaaaccgga atgtcggcaa aacaaacggc ttttgctatg
    14881 cgcatgttgc ctgcacaaat gacggatatt gttgttgggt tgtccactgg tcagtcgcca
    14941 tttatggtgt taatgcagca gggcggccag ctaaaagata tgttcggtgg gattggcccg
    15001 gcgatcaaag gtgtaggttc ttatgtgctg ggattaatta atccttttac cctggccgca
    15061 gcagccgttg gggtcttagg actggcttac tataaaggct ctcaggagca ggacgaattt
    15121 aataaatctc ttattcttac cggaaatcag ttggggacaa ccagcgggca attgggcgat
    15181 atagctcaac gtgccgggaa tgcggctgat tcgacaactg gtgctgcggc ggcagtatta
    15241 aatcagcttg tgcgttcggg aaaggtagcg agcagttcgc tggagcaagt gacgacagcg
    15301 atagtaaaaa cgagcgaagt aacaggaata tcaaccgaac aactggttaa tgacttcaat
    15361 gaaattgcaa aggatcctgt cagtgccata tcaaaactta atgatcagta ccattttctg
    15421 acacttgcga cttataacca gattaaggcg ctacaggatg aagggaacca gcaggaggcc
    15481 gcccgcattg cgacagaaga atactcatcc tcaatgatcc agcgcaccaa ccagattaaa
    15541 gaaaatcttg gttatcttga gactgcatgg aaagctgtcg cagactccgc aaaatgggca
    15601 tgggattcca tgctggatat tggccgtgag gcctcccttg atcaaaaaat ctcagatgtt
    15661 ctccgtcaaa ttgatgaaat agaaaaaaat acccgacccg gagttttcgg gttaggtggc
    15721 attggagatg gcggagctca aaataaaagg ctggcacgat taaagcagca attgggcgta
    15781 cttcaagcag aaaaaattgc tcaggacgta ctaaattcat caataaacga ttacaacaag
    15841 cgacaacagg aaggaattga actcagacag agagcagatg ccttttcaaa acaatatcag
    15901 acccgggagc agcagagagc tagtgaactt gcaaaactgg aaaagctaaa gaatcagtat
    15961 tcaaaggaag aatataataa tcttatcgct caaataaatg agcgttataa agatccaaag
    16021 caaccaaagg cgaaaggtta ttctgatgat gcagcccaac gaatgattga tcatctgaat
    16081 caacagaatg cgttactaag ttcacaagct gagttgaccg ttaaattaag ttcctctgaa
    16141 caggaactgg ttaagtggcg tcagcggatt gccgacctag agtcacgacc atcatcgaaa
    16201 ttaacccagg atcagaaatc gcttctctta caccgggaag aaataaccac gttgatggag
    16261 aaaaatgttg cgattgaaaa aaataacagg ctaatcaagg aatccgccga aataacagca
    16321 tggcgtgatt cattgcaggc ttcgattgat aatcgtcagc aggggtatga tattcagatt
    16381 gctggttatg gggttggcga taaaaatcag caacgccagc aggaattact gcggattgaa
    16441 catggatata acaatcagcg tctgcaactt gaacgtgact atgcagataa atcccgtgga
    16501 atgtcagatc atgtttttca agagaaaatg caggctctga atgatgctct ggaacgggaa
    16561 aaagaaattg tcagacagaa aaacgagcag ctcgatattc aggcaggaga ctgggttagt
    16621 ggtgcctccc agggattcaa taactggctg gatgacacta aggatatcag tgagcagata
    16681 aaatcaacca cgactcagat gtttgatggg atgaccgatg cgctgggtga ttttgtcacg
    16741 acaggcaagg caaattttcg ttctttcgct acttccgtga tttcggatct tagtcgaata
    16801 gcattaaagg cttcaattac tgggattttc gacagcatta gtaacagttc ttctgggggg
    16861 attttaggaa ctatcgggag tgctattagt aaatttattc cgaatgcaaa gggcggtgtt
    16921 tatgagtctc cgtcattgag cacgtattcg aacggtattt atgattcccc gcaatttttt
    16981 gcttttgcaa aaggggctgg tgtttttggt gaggctggac cggaagccat tatgccatta
    17041 acacgaactt ccgatggttc tcttggtgtc agagctatta atagtaaaag tggtaatgga
    17101 ggcggagata ttacctatgc ccctgtatac caaatcacta ttcaaaatga cggccagaat
    17161 ggagagattg gccctcaggc aataaaagca cttatgggga tggttgatca gcgggtgcag
    17221 ggcaatctgt taaatatgcg acgtgatggg ggaatgttaa gtggctaata cggaagaatt
    17281 tcactggtta ccagaggatg gaatgaaaac agaaaataaa ccatcgataa aaactgtaag
    17341 atttggcgat ggttatgagc aacgaagtcc aaatggactt aatcattctc tgcgcgtttt
    17401 cacctgtgat ttcaaagttg aggcgaatga acgtgattca tttgaaaaat ttttagcccg
    17461 gcatgaaggc tataaatctt ttttttggcg cccgccgggt attaacagaa aaatcagagt
    17521 ggtgtgtcga acgtggtcag cgacagaaca tatcacctat accgattttt cgtgtcagtt
    17581 tgacgaagtg gtgatctgat gcaggacata caacaggaaa cgctgaatga atgcattcgt
    17641 gcggagcagt cggccagcgt ggtgctctgg gaaatcgacc tgaccgaggt aggcggggat
    17701 cgctactttt tctgtaatga gcagaacgaa aaaggtgagt ctgtcacctg gcagagtcgg
    17761 cagtatcagg cctatcccat tcaggggagt ggatttgaga tgaacggcaa gggcagtgct
    17821 gcccgtccga cactgacggt ctctaacctg cacggcatga tcaccgggat ggcggaagat
    17881 ctgcagagtc tggtcggcgg aacggtggtc cggcgtaagg tttacgcccg ctttctggat
    17941 gcggtgaact tcgtcaacgg aaacagcgaa gccgatccgg agcaggaggt gatcagccgc
    18001 tggcgcatcg agcagtgcag cgaactgagc gcggtcagtg cctcttttgt actgtccacg
    18061 ccgactgaaa cggatggcgc tgtttttccg ggacgtatca tgctggccaa cacctgcacc
    18121 tggacctatc gcggcgatga gtgcggttat agcggtccgg cggtcgcgga tgaatatgac
    18181 cagccgacgt ccgatatcac gaaggataaa tgcagcaaat gcctgagtgg ctgtaagttt
    18241 cgcaataatg tcggcaactt tggcagcttc ctttccatta acaaactttc gcagtaaatc
    18301 ccatgacaga gacagaatca gcgattctgg cgcacgcccg gcgatgtgcg ccagcggagt
    18361 cgtgcggctt cgtggtgaga acgccggagg aggaaagata ttttccctgc gtgaatatct
    18421 ccggtgagcc ggaggagtat ttccggatgt cgccggagga ctggctgcag gccaaaatgc
    18481 agggagagat tgtggcgctg gtccacagcc accccggtgg tctgccctgg ctgagtgagg
    18541 ccgaccggcg gctgcaggta cagagtgatt tgccgtggtg gctggtctgc cggggggcga
    18601 ttcataaatt ccgctgtgtg ccgcatctca ccgggcggcg ctttgagcac ggggtaacgg
    18661 actgttacac gctgttccgg gacgcttacc atctggcggg aattgagatg ccggattttc
    18721 atcgcgggga tgactggtgg cgtaacggtc agaatctcta tctggataat ctggaggcca
    18781 cagggctgta tcaggtgccg ttgtcagcgg cgcagccggg cgatgtgctg ctgtgctgtt
    18841 ttggttcatc ggtgccgaat catgccgcca tttactgtgg tgacagcgag ctgctgcacc
    18901 atattcctga acaactgagc aaacgagaga ggtacaccga caaatggcag cgacgcacac
    18961 actccctctg gcgtcaccgg gcatggcacg catctgcctt tacggggatt tacaacgatt
    19021 tggccgccgc atcgaccttc gagtgaaaac ggggtccgaa gccatccggg cgctggccat
    19081 gcagatcccg gcatttcgtc agaaactgag cgacggctgg taccaggtac gcattgccgg
    19141 gcgtgatgca ggtgaaaccg aattgtctgc ccgtcttaat gagccgctgg aaaatggtgc
    19201 cgtgatccat atcgtgccgc gtctggcagg agccaaaagt ggcggtgtgt ttcaggctgt
    19261 gctgggggcg gctgttatgg cggttgctat atggatgccg ggggtaggaa ttatggcgag
    19321 taatctgctg ttttctctcg gtgccagtat gacgcttggc ggtgttgcac agatgctggc
    19381 ccctaaaccc aaaacccccc gcacacagac aacggataac ggcaaacaga acacctattt
    19441 ttcttctctg gataacatgg ttgcccaggg caatgttctg ccggttctgt acggtgaaat
    19501 gcgcgtgggg tcacgcgtgg tttctcagga gatcagcacg gcagacgaag gggatggtgg
    19561 tcaggttgtg gtgattggtc gctgatgcaa aatgttttat gtgaaaccgc ctccgggcgg
    19621 ttttatcgtt tatggagcat gacgaatggg taaaggcagc agtaaggggc ataccccgcg
    19681 cgaagcgaag gacaacctga agtccacgca gctgctgagt gtgatcgatg ccatcagcga
    19741 agggccg
//
LOCUS       DAPGWD010000069        19432 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPGWD010000069 DAPGWD010000000
VERSION     DAPGWD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.069"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            42..1019
                     /locus_tag="RHX88_002507"
     CDS             42..1019
                     /locus_tag="RHX88_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDV2231182.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLSAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1089..1571)
                     /locus_tag="RHX88_002508"
     CDS             complement(1089..1571)
                     /locus_tag="RHX88_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDV2231183.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1806..4388
                     /gene="leuS"
                     /locus_tag="RHX88_002509"
     CDS             1806..4388
                     /gene="leuS"
                     /locus_tag="RHX88_002509"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDV2231184.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4403..4984
                     /gene="lptE"
                     /locus_tag="RHX88_002510"
     CDS             4403..4984
                     /gene="lptE"
                     /locus_tag="RHX88_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDV2231185.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4984..6015
                     /gene="holA"
                     /locus_tag="RHX88_002511"
     CDS             4984..6015
                     /gene="holA"
                     /locus_tag="RHX88_002511"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDV2231186.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENSAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6017..6658
                     /gene="nadD"
                     /locus_tag="RHX88_002512"
     CDS             6017..6658
                     /gene="nadD"
                     /locus_tag="RHX88_002512"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDV2231187.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6682..7293
                     /locus_tag="RHX88_002513"
     CDS             6682..7293
                     /locus_tag="RHX88_002513"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDV2231188.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7553..7870
                     /gene="rsfS"
                     /locus_tag="RHX88_002514"
                     /gene_synonym="rsfA"
     CDS             7553..7870
                     /gene="rsfS"
                     /locus_tag="RHX88_002514"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDV2231189.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7874..8341
                     /gene="rlmH"
                     /locus_tag="RHX88_002515"
     CDS             7874..8341
                     /gene="rlmH"
                     /locus_tag="RHX88_002515"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDV2231190.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8372..10273
                     /gene="mrdA"
                     /locus_tag="RHX88_002516"
     CDS             8372..10273
                     /gene="mrdA"
                     /locus_tag="RHX88_002516"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDV2231191.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10276..11388
                     /gene="mrdB"
                     /locus_tag="RHX88_002517"
                     /gene_synonym="rodA"
     CDS             10276..11388
                     /gene="mrdB"
                     /locus_tag="RHX88_002517"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDV2231192.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11399..12487
                     /gene="rlpA"
                     /locus_tag="RHX88_002518"
     CDS             11399..12487
                     /gene="rlpA"
                     /locus_tag="RHX88_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDV2231193.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12626..13837
                     /gene="dacA"
                     /locus_tag="RHX88_002519"
     CDS             12626..13837
                     /gene="dacA"
                     /locus_tag="RHX88_002519"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDV2231194.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13948..14211
                     /locus_tag="RHX88_002520"
     CDS             13948..14211
                     /locus_tag="RHX88_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDV2231195.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14312..14953
                     /gene="lipB"
                     /locus_tag="RHX88_002521"
     CDS             14312..14953
                     /gene="lipB"
                     /locus_tag="RHX88_002521"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDV2231196.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15212..16165
                     /locus_tag="RHX88_002522"
     CDS             15212..16165
                     /locus_tag="RHX88_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV2231197.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16374..17339
                     /gene="lipA"
                     /locus_tag="RHX88_002523"
     CDS             16374..17339
                     /gene="lipA"
                     /locus_tag="RHX88_002523"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDV2231198.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17440..17643)
                     /gene="tatE"
                     /locus_tag="RHX88_002524"
     CDS             complement(17440..17643)
                     /gene="tatE"
                     /locus_tag="RHX88_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDV2231199.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17772..18560)
                     /locus_tag="RHX88_002525"
     CDS             complement(17772..18560)
                     /locus_tag="RHX88_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDV2231200.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18653..19036
                     /gene="crcB"
                     /locus_tag="RHX88_002526"
     CDS             18653..19036
                     /gene="crcB"
                     /locus_tag="RHX88_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDV2231201.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19090..19299)
                     /gene="cspE"
                     /locus_tag="RHX88_002527"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19090..19299)
                     /gene="cspE"
                     /locus_tag="RHX88_002527"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV2231202.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT         4869 a         4920 c         4927 g         4716 t
ORIGIN      
        1 aaaatataat atgttttatc tgcaaccata aggaaatgat aatgattttc acgtcaagtt
       61 gctgcgataa tttatcaata gatgagatta tcgaacgtgc tgaaaaaggc gattgcgagg
      121 ctcaatatat tgttgggttt tattataatc gcgatagcgc aattgattct ccggacgacg
      181 aaaaagcctt ttactggctg aagctgtccg ctgagcaagg tcattgtgaa gcacagtatt
      241 ccttagggca aaagtatacc gaggataaaa gccgtcataa agataatgag caagccatct
      301 tctggctgaa aaaagctgcc ctacaaggcc atactttcgc ttccaacgcc cttggctgga
      361 cactggatcg tggagaagcc cccaattata aagaagcggt tgtctggtat cagatagccg
      421 cggagagcgg aatgtcttat gcgcaaaata atcttgggtg gatgtacaga aatggcaacg
      481 gagtcgcaaa agactatgcg ctggcatttt tctggtacaa acaagctgca ttacaaggcc
      541 atagtgacgc gcaaaacaat ctggccgatc tttatgaaga cggaaaaggc gttgctcaaa
      601 acaagacact cgccgcattc tggtatttga aaagcgcaca gcagggtaat cggcacgccc
      661 agtttcaaat tgcatgggat tataacgctg gcgaaggggt ggaccaggac tataagcaag
      721 cgatgtactg gtatctgaag gctgccgctc aggaaagcgt cggcgcttac gtcaacatcg
      781 gttatatgta taaacacgga caaggcgttg agaaagatta tcaggctgcc tttgaatggt
      841 ttacgaaagc cgctgaatgc aatgacgcca ctgcctggta taacctggcc attatgtatc
      901 actacggaga aggaagacct gtcgatctcc gacaggctct cgacctgtat cgtaaagttc
      961 agtcatccgg aaccagagat gtcagtcagg aaattcgtga gactgaagat ttactgtaga
     1021 gataaaaaca agggccgggg aatttcattc ggcccatatt ctctttattg ctgtgcgaaa
     1081 tcagactatt acggctcaaa cgggcgtctc tggaactggt catgaccaca tttcgggcat
     1141 agcgtcagca cttccggtgt gtagatcggg agatggaagt gacatttctc gcagaccaga
     1201 tttcccagcc cgaccacttc tccgctgtga taaaccccat gatgattgag gtcttggaaa
     1261 acttcgcgcc attcaagctg cgttttatcg gtgatatctg ccagctcctg ccataagctt
     1321 tctttaatca cccgcataaa gacgctgtca gattcttctt tcaggctctc ttcatagctc
     1381 atggcgaact cttccaggtc acgtcttaca gctcgcgtca gctcatcgac ctcggttcgc
     1441 gttaactccc ctgtttttat tacgcgctcg cgcgcctgtt ccaccagtgc gtcgatatca
     1501 cgttcgccat tgcgcaggcg ttcgctcagt gacgcgacca gttcacggta atattgagca
     1561 accttgttca tcgtttcatc tcccgggtcg ttaactgact ataataatag acgttctttg
     1621 ttccttgctt tgctaatacg cccacaaact ctgtaaatta atgtatgaat aatccgggca
     1681 tcatttcaac gtcgagtgcg cgaaaggctg ttttgacgcg gacgtttggg ctatgctatg
     1741 cggatctgaa aaaccacctc aacgctacat ttgtagccgt attgaaaaca ggaccactgg
     1801 ctgccatgca agagcaatac cgcccggaag agatagaatc caaagtacag cttcattggg
     1861 atgagaagcg cacatttgaa gtaaccgaag acgagagcaa agagaagtat tactgcctgt
     1921 ctatgcttcc ctatccttct ggtcgactac acatgggcca cgtacgtaac tacaccatcg
     1981 gtgacgtgat cgcccgctac cagcgtatgc tgggcaaaaa cgtcctgcag ccgatcggct
     2041 gggacgcgtt tggtctgcct gcggaaggcg cggcggtgaa aaacaacacc gctccggcac
     2101 cgtggacgta cgacaacatc gcgtatatga aaaaccagct caaaatgctg ggctttggtt
     2161 atgactggag ccgcgagctg gcaacctgta cgccggaata ctaccgttgg gaacagaaat
     2221 tcttcaccga gctgtataaa aaaggcctgg tatataagaa gacttctgcg gtcaactggt
     2281 gcccgaacga ccagaccgta ctggcgaacg aacaagttat cgacggctgc tgctggcgct
     2341 gcgataccaa agttgaacgt aaagagatcc cgcagtggtt tatcaaaatc actgcttacg
     2401 ctgacgagct gctcaacgat ctggataaac tggatcactg gccagacacc gttaaaacca
     2461 tgcagcgtaa ctggatcggt cgttccgaag gcgtggagat caccttcaac gttaacgact
     2521 atgacaacac gctgaccgtt tacactaccc gcccggacac ctttatgggt tgtacctacc
     2581 tggcggtagc tgcgggtcat ccgctggcgc agaaagcggc ggaaaataat cctgaactgg
     2641 cggcctttat tgacgaatgc cgtaacacca aagttgccga agctgaaatg gcgacgatgg
     2701 agaaaaaagg cgtcgatact ggctttaaag cggttcaccc attaacgggc gaagaaattc
     2761 ccgtttgggc agcaaacttc gtattgatgg agtacggcac gggcgcagtt atggcggttc
     2821 cgggtcacga ccagcgcgac tacgagtttg cctctaaata cggcctgaac atcaaaccgg
     2881 ttatcctggc agctgacggc tctgagccag atctttctca gcaagccctg actgaaaaag
     2941 gcgtgctgtt caactctggc gagttcaacg gtcttgacca tgaagcggcc ttcaacgcca
     3001 tcgccgataa actgactgcg atgggcgttg gcgagcgtaa agtgaactac cgcctgcgcg
     3061 actggggtgt ttcccgtcag cgttactggg gcgcgccgat tccgatggtg acgctggaag
     3121 acggtaccgt aatgccgacc ccggacgacc agctgccggt gatcctgccg gaagatgtgg
     3181 taatggacgg cattaccagc ccgattaaag cagatccgga gtgggcgaaa actaccgtta
     3241 acggtatgcc agcactgcgt gaaaccgaca ctttcgacac ctttatggag tcctcctggt
     3301 actatgcgcg ctacacttgc ccgcagtaca aagaaggtat gctggattcc gaagcggcta
     3361 actactggct gccggtggat atctacattg gtggtattga acacgccatt atgcacctgc
     3421 tctacttccg cttcttccac aaactgatgc gtgatgcagg catggtgaac tctgacgaac
     3481 cagcgaaaca gttgctgtgt cagggtatgg tgctggcaga tgccttctac tatgttggcg
     3541 aaaacggcga acgtaactgg gtttccccgg ttgatgctat cgttgaacgt gacgagaaag
     3601 gccgtatcgt gaaagcgaaa gatgcggcag gccatgaact ggtttatacc ggcatgagca
     3661 aaatgtccaa gtcgaagaac aacggtatcg acccgcaggt gatggttgaa cgttacggcg
     3721 cggacaccgt tcgtctgttt atgatgtttg cttctccggc tgatatgact ctcgaatggc
     3781 aggaatccgg tgtggaaggg gctaaccgct tcctgaaacg tgtctggaaa ctggtttacg
     3841 agcacacagc aaaaggtgat gttgcggcac tgaacgttga tgcgctgact gaagatcaga
     3901 aagcgctgcg tcgcgatgtg cataaaacga tcgctaaagt gaccgatgat atcggccgtc
     3961 gtcagacctt caacaccgca attgcggcga ttatggagct gatgaacaaa ctggcgaaag
     4021 caccaaccga tggcgagcag gatcgcgctc tgatgcagga agcactgctg gccgttgtcc
     4081 gtatgcttaa cccgttcacc ccgcacatct gcttcacgct gtggcaggaa ctgaaaggcg
     4141 aaggcgatat cgacaacgcg ccgtggccgg ttgctgacga aaaagcgatg gtggaagact
     4201 ccacgctggt cgtggtgcag gttaacggta aagtccgtgc caaaatcacc gttccggtgg
     4261 acgcaacgga agaacaggtt cgcgaacgtg ctggccagga acatctggta gcaaaatatc
     4321 ttgatggcgt tactgtacgt aaagtgattt acgtaccagg taaactcctc aatctggtcg
     4381 ttggctaagc gcgggaggaa gcgtgcgata tctggcaaca ttgttgttat ctctggcggt
     4441 gttaatcacc gccgggtgtg gctggcatct gcgtgatacc acgcaggttc cttccactat
     4501 gaaggtcatg atcctggact caggcgatcc gaacgggcca ttaagccgtg cggtgcgtaa
     4561 ccagttacgt ctgaatggtg tcgagttgct tgataaagaa accacgcgta aggacgttcc
     4621 atccttgcgt ttgggtaaag tgagcatcgc gaaagatacc gcatcggtat tccgtaacgg
     4681 tcaaacagca gagtatcaga tgatcatgac ggttaatgcg accgtgttga tccccggccg
     4741 tgatatctac ccgattagcg ccaaagtctt ccgttcgttc ttcgataacc cgcaaatggc
     4801 gttagcgaaa gataacgaac aagacatgat cgtaaaagag atgtacgacc gtgctgccga
     4861 acagctgatt cgtaagctgc caagcatccg tgctgcggat attcgttccg acgaagaaca
     4921 gacgtcgacc acaacggata ctccggcaac gcctgcacgc gtctccacca cgctgggtaa
     4981 ctgatgattc ggttgtaccc ggaacaactc cgcgcgcagc tcaatgaagg gctgcgcgcg
     5041 gcgtatcttt tacttggtaa cgatcctctg ttattgcagg aaagccagga cgctgttcgt
     5101 caggtagctg cggcacaagg attcgaagaa caccacactt tttccattga tcccaacact
     5161 gactggaatg cgatcttttc gttatgccag gccatgagtc tgtttgccag tcgacaaacg
     5221 ctattgctgt tgttaccaga aaacggaccg aatgcggcga tcaatgaaca acttctcaca
     5281 ctcaccggac ttctgcatga cgacctgctg ttgatcgtcc gcggtaataa attaagcaaa
     5341 gcgcaagaaa attccgcctg gtttactgcg cttgcgaatc gcagcgtgca ggtgacctgt
     5401 cagacaccgg agcaggctca gcttccccgc tgggttgctg cgcgcgcaaa acagctcaac
     5461 ttagaactgg atgacgctgc aaatcaggtg ctctgctact gttatgaagg taacctgctg
     5521 gcgctggctc aggcactgga gcgtttatcg ctgctctggc cagacggcaa attgacatta
     5581 ccgcgcgttg aacaggcggt gaatgatgcc gcgcatttca ccccttttca ttgggttgat
     5641 gctttgttga tgggaaaaag taagcgcgcg ttgcatattc ttcagcaact gcgtctggaa
     5701 ggcagcgaac cggttatttt gttgcgcaca ttacaacgtg aactgttgtt actggttaac
     5761 ctgaaacgcc agtctgccca tacgccactg cgtgcgttgt ttgataagca tcgggtatgg
     5821 cagaaccgcc ggggcatgat gggcgaggcg ttaaatcgct taagtcagac gcagttacgt
     5881 caggccgtgc aactcctgac acgaacggaa ctcaccctca aacaagatta cggtcagtca
     5941 gtgtgggcag agctggaagg gttatctctt ctgttgtgcc ataaacccct ggcggacgta
     6001 tttatcgacg gttgatatga aatctttaca ggctctgttt ggcggcacct ttgatccggt
     6061 gcactatggt catcttaaac ccgtggaaac gctggcgaat ttgattggtc tgacgcgggt
     6121 cacaatcatc cctaataatg ttcctccgca tcgtccccag ccggaagcga acagcgtgca
     6181 gcgtaaacac atgcttgaac tggcgattgc cgacaagcca ttatttactc ttgatgaacg
     6241 cgagctaaag cgcaatgccc cctcttacac tgcgcaaaca ctgaaagagt ggcggcagga
     6301 acaagggccg gacgtgccgc tggcgtttat tattggtcag gattcattgc tgacctttcc
     6361 gacctggtac gaatacgaaa cgatactcga caatgcacat ttgatcgtct gtcggcgtcc
     6421 aggttaccca cttgaaatgg cgcaaccgca ataccagcaa tggctggaag atcatttgac
     6481 acataacccg gaagatcttc accttcagcc tgccggtaaa atttatctgg ctgaaacgcc
     6541 gtggtttaac atctcggcga ccatcatccg cgaacgtttg caaaacggtg aatcgtgtga
     6601 ggatttattg ccggaaccgg tactgactta cattaaccaa caaggcttgt atcgctgata
     6661 cctgtcgtta cggagggcat gatgcgactg tggttaattc gtcatggtga aacgcaagcg
     6721 aatatcgatg gtctttacag cggtcatgcg cccacccccc tgaccgcgcg cggtattgag
     6781 caagcgcaaa atctgcatac actgctagat gatgtttcct ttgacctggt tttatgcagt
     6841 gaactggaac gggcacagca taccgcgcga cgggttctca gtgaccgcca gctccccgtg
     6901 caaatcatcc ctgaactcaa cgaaatgttt tttggcgact gggaaatgcg acatcatcgc
     6961 gacctcatgc aagaagatgc cgaaaactat agcgcgtggt gcaatgactg gcagcatgct
     7021 atccccacga acggtgaagg atttcaggca ttttcgcaac gtgtggaaag atttatcgca
     7081 aggcttagtg aatatcagca ctatcagaat attttaatcg tcagccatca gggcgtactg
     7141 agtctgttaa ttgctcgtct aattggcatg cctgccgaat ctatgtggca ttttcgcgtt
     7201 gaccaggggt gttggagcgc cattgatatc aaccaaaaat tcgcgacgct acgcgtcctc
     7261 aatagccgtg ccatcggggt cgagaatgca tgacttttct gtttttttac gggtaagccg
     7321 caacgaccat tgacagcccc gggcaggctg atattctccg cagccagact ttttccgcca
     7381 gacacgactt tgtagaaatt gttttacaaa aatggcgatg caatctgcgg cgcggggtgg
     7441 gatgatagcc cactttcgaa agccgattcg gcgacaattg tcccgaaatc gcctctggtt
     7501 caggtatact gacagaccat ttttatctat ttgattcacc cagggggaaa acttgcaggg
     7561 taaagcactc caggattttg ttatcgacaa aattgatgac ctcaaaggtc aggacatcat
     7621 cgccttagac gttcagggca aatccagcat caccgactgc atgatcatct gtacgggtac
     7681 gtccagccgt catgttatgt ccattgctga ccacgttgtg caggagtctc gcgcagcggg
     7741 cctgttaccg ctcggcgtag aaggtgaaaa cagcgccgac tggattgtcg tggatttggg
     7801 cgatgtgatt gtccatgtca tgcaggaaga gagccgtcgc ctgtatgaac tggaaaaact
     7861 ctggagttaa tgcgtgaagc tgcaacttgt cgccgtggga acgaaaatgc cggactgggt
     7921 acaaaccggt tttaccgagt acctgcgtcg ttttccgaaa gatatgccct tcgagctgat
     7981 tgaaattccg gccggaaaac gcggcaagaa tgcggacatc aagcgcatac tcgacaaaga
     8041 gggtgagcag atgttggcgg ccgcaggcaa aaaccgcatt gtcaccctcg atattccagg
     8101 caagccctgg gatacgccgc agttagccgc tgagctggaa cgctggaagc tggatggtcg
     8161 cgacgtcagt ctactgattg gcgggcctga agggttgtcg cctgcctgta aagcggcggc
     8221 ggagcagagc tggtcgctgt cggcgcttac cctcccccat ccgctggttc gcgtgctggt
     8281 cgcagagagt ctgtaccggg cgtggagcat caccaccaac catccttatc accgtgagtg
     8341 ataagggagc tttgagtaga aaacgcagcg gatgaaacta cagaactctt ttcgcgacta
     8401 tacggcagag tccgcgctgt ttgtgcgccg ggcgctggtc gcctttttgg ggattttgct
     8461 gctgaccggc gtgcttatcg ccaacctgta taatctgcaa attgttcgct ttaccgacta
     8521 ccagactcgc tctaatgaaa accgcattaa gctggtgcct atcgcgccca gccgcggcat
     8581 tatctacgac cgtaatggta tccctctggc cctcaaccgc actatctacc agatagaaat
     8641 gatgccagag aaagtcgata acgtgcagca aacgctggac gctttgcgca gcgtggttga
     8701 tctgaccgat gacgatattg ctgcattccg aaaagagcgc gcacgttcac accgtttcac
     8761 ctctattccg gtgaaaacta acctgaccga agtacaagta gctcgctttg ccgtcaatca
     8821 gtaccgtttt ccgggtgtcg aagttaaagg ctataaacgt cgttactatc cttacggttc
     8881 ggcgttgacc cacgtcatcg gctatgtgtc gaaaatcaac gataaagacg tcgaacgcct
     8941 gaataatgac ggcaaactgg ccaactatgc ggcaacgcat gatatcggta agctgggcat
     9001 tgagcgttac tatgaagacg tgctgcacgg tcagaccggt tatgaagagg ttgaagttaa
     9061 caaccgtggg cgtgttatcc gccagttaaa agaagtacca ccgcaagccg gacacgatat
     9121 ttacctgacg ctggatctca aactccagca atatattgaa acgctgctgg cgggtagccg
     9181 cgcagctgtg gttgtcaccg atccgcgtac aggtggggtg ctggcgctgg tttccacgcc
     9241 tagttatgac ccaaacctgt ttgttgacgg tatctccagc aaagattatt ccgccttgtt
     9301 gaacgacccg aatacaccgc tggtgaaccg cgccacacag ggggtttatc ctcccgcgtc
     9361 tacagttaaa ccctatgtgg cggtttcggc attgagcgcc ggggtgatca cgcgcaatac
     9421 gacgctgttt gacccaggct ggtggcaact gccaggttcg gaaaaacgtt atcgtgactg
     9481 gaaaaaatgg ggccacgggc gtctgaatgt cacaagatcg ctggaagaat ctgcggatac
     9541 cttcttctat caggtggcct acgatatggg gatcgatcgc ctctccgaat ggatgggtaa
     9601 attcggttat ggtcattaca ccggtatcga cctggcggaa gaacgttccg gcaacatgcc
     9661 tacccgcgaa tggaaacaga aacgctttaa aaaaccgtgg tatcagggtg acaccattcc
     9721 ggttggtatc ggtcagggtt actggacagc gaccccaatc cagatgagta aggcactgat
     9781 gatcctgatt aatgacggta tcgtgaaggt tcctcatttg ctgatgagca ccgccgaaga
     9841 cggcaaacag gtgccatggg tacagccgca tgaaccgccc gtcggcgata ttcattccgg
     9901 ttactgggag ctggcgaaag acggtatgta cggtgttgct aaccgcccta acggtacggc
     9961 gcataaatac tttgccagcg caccgtacaa aattgcggcg aaatccggta ccgctcaggt
    10021 cttcggtctg aaagcgaacg aaacctataa tgcgcacaaa attgccgagc gtttacgtga
    10081 ccacaaactg atgaccgcct ttgcgccata caacaatccg caagtggctg tcgccatgat
    10141 tctggagaac ggtggtgcgg gtccggcggt tgggacactg atgcgccaga tcctcgacca
    10201 cattatgctg ggtgataaca acaccgatct gcctgcggaa aatccagcgg ttgccgcagc
    10261 ggaggaccat taatcatgac ggataatccg aataaaaaaa cattctggga taaagtccat
    10321 ctcgatccca caatgctgct gatcttactg gcattgctgg tttacagcgc cctggttatc
    10381 tggagcgcca gcggtcagga tattggcatg atggagcgta aaatcggcca aatcgcgatg
    10441 ggtctggtca tcatggtggt gatggcgcaa attcctccac gcgtttatga aggctgggcc
    10501 ccctatctct atatcatctg tattattttg ctggtggcgg tagatgcttt cggtgccatc
    10561 tctaaaggtg ctcaacgctg gctggacctc ggtattgttc gttttcagcc gtcggaaatt
    10621 gccaaaatag ccgtaccact gatggttgcg cgctttatca accgcgacgt ttgcccgcca
    10681 tcgttgaaga acactggcat cgcgctggtg ctgatattta tgcccacgct gctggtggct
    10741 gcacagcctg acctgggaac atcaatcctc gttgcgcttt ccggtctgtt tgtactgttc
    10801 ctctctggcc ttagctggcg tctgattggc gtcgcagtag tgctggtggc ggcgttcatt
    10861 ccgattctgt ggttcttcct gatgcatgat taccagcgcc agcgcgtaat gatgctcctg
    10921 gacccggaat cagacccact cggcgcgggc tatcacatta ttcagtctaa aattgctatt
    10981 ggctccggcg gattacgcgg caaaggctgg ctgcacggca ctcagtcaca gcttgaattt
    11041 ctccccgaac gccataccga ctttatcttc gcggtactgg cggaagagct gggattagtg
    11101 ggcattttga ttctgctcgc tctctacatt ctgctgatca tgcgcgggct gtggatagcc
    11161 gccagagcgc aaaccacctt tggtcgcgtc atggctggtg gcttaatgct gatattattc
    11221 gtttatgtct tcgtaaatat tggtatggta agcggtattc tgccggttgt aggggttccg
    11281 ctcccactgg tcagttatgg aggatcggcg ctaattgtgc tgatggctgg gttcgggatt
    11341 gtaatgtcaa tccacaccca caggaaaatg ttgtcgaaaa gcgtgtaaga ggtgcgcaat
    11401 gcgtaagcag tggctcggga tctgcatcgc ggcaggaatg ctcgcggcat gtacaagcga
    11461 tgatggtcag caacagacgg taagtgtacc gcagcctgcg gtatgtaacg gccccatagt
    11521 tgaaattagc ggggcggatc cgcgtttcga accactgaac gcgacggcaa atcaggatta
    11581 ccagcgcgac ggtaaaagct acaaaatcgt gcaggatccg tctcgattta gccaggcggg
    11641 actggcggca atctatgatg ccgaaccagg cagtaacctg acggcctctg gcgaagcttt
    11701 cgatccgaca cagctgacgg cggcccatcc aacgcttccg atccccagct acgccagaat
    11761 cactaacctg gctaacgggc gaatgatcgt ggtgcgcatt aatgatcgcg gcccttacgg
    11821 caacgaccgc gttatttcgc tttctcgcgc ggcagctgac cgtcttaaca cgtcaaacaa
    11881 caccaaagtt cgtatcgatc cgattattgt cgcccaggat ggttcgcttt ctggtcctgg
    11941 tatggcttgt accacagtcg ccaaacagac ttacgccctg ccagcacctc ccgatttaag
    12001 cggtggcgcg ggaacaagtt cagtgtctgg cccgcagggt gacattcttc cggtcagtaa
    12061 ttcgacgcta aaaagcgaag atccgaccgg cgcgccggta accagtagcg gtttcctcgg
    12121 cgctccaacg accttagcgc ctggtgtact ggaaggcagc gaaccgacgc ctgcgccaca
    12181 gcccgttgtt acagctccgt cgacaacgcc tgcaacctcg cctgcaatgg tgacaccgca
    12241 agccgcctcg caaagcgcca gcggcaactt tatggtgcaa gtcggggccg ttagcgatca
    12301 ggctcgtgcg caacagtacc aacagcaact gggacagaag ttcggcgtcc ccggtcgcgt
    12361 aactcaaaat ggcgcggtct ggcggatcca gcttggccca ttcgccagca aagccgaagc
    12421 cagtaccttg cagcaacgtt tgcaaaccga agcccaatta cagtcattta ttactaccgc
    12481 gcagtagcgt aaagcaggca tctgatgtgt caatttctgt aaatgacgtt aacaaagtca
    12541 cgcggaaagt cagatgcctg ccggtagtgc atttgctata gtagggcact tttttaattc
    12601 catcacggat gtcgtagttc agaccatgaa taccattttt tccgctcgta tcatgaagcg
    12661 cctggcgctc accacggctc tttgcacagc ctttatctct gctgcacatg ccgatgacct
    12721 gaatatcaaa actatgatcc cgggtgtacc gcagatcgat gcggagtcct acatcctgat
    12781 tgactataac tccggcaaag tgctcgccga acagaacgca gatgtccgcc gcgatcctgc
    12841 cagcctgacc aaaatgatga ccagttacgt tatcggccag gcaatgaaag ccggtaaatt
    12901 taaagaaact gatttagtca ctatcggcaa cgacgcatgg gccaccggta acccggtgtt
    12961 taaaggttct tcgctgatgt tcctcaaacc gggcatgcag gttccggttt ctcagctgat
    13021 ccgcggtatt aacctgcaat cgggtaacga tgcttgtgtc gccatggccg attttgccgc
    13081 tggtagccag gacgcttttg ttggcttgat gaacagctac gttaacgcac tgggcctgaa
    13141 aaatacccac ttccagacgg tacatggtct ggatgctgat ggtcagtaca gctccgcgcg
    13201 agatatggcg ctgatcggcc aggcattgat ccgtgacgta ccgaatgaat actcgatcta
    13261 taaagaaaaa gaatttacgt ttaacggtat tcgccagctg aaccgtaacg gcctgttatg
    13321 ggataacagc ctgaatgtcg acggcatcaa aaccggacac actgacaaag caggttacaa
    13381 ccttgttgct tctgcgactg aaggccagat gcgcttgatc tctgcggtga tgggcggacg
    13441 tacttttaaa ggccgtgaag ccgaaagtaa aaaactgcta acctggggct tccgtttctt
    13501 cgaaaccgtt aacccactga aagtaggtaa agagttcgcc tctgaaccgg tttggtttgg
    13561 tgattctgat cgcgcttcgt taggtgttga taaagacgtg tacctgacca ttccgcgtgg
    13621 tcgcatgaaa gatctgaaag ccagctatgt gctgaacagc agtgaattgc atgcgccgct
    13681 gcaaaagaat caggtcgtcg gaactatcaa cttccagctt gatggcaaaa cgatcgaaca
    13741 acgcccgctg gttgtgttgc aagaaatccc ggaaggtaac ttcttcggca aaatcattga
    13801 ttacattaaa ttaatgttcc atcactggtt tggttaaaaa ttgaacactt gaaagtgtaa
    13861 tttccgtccc catatactaa gcatcagtaa aaaaactccc gccttctggc gggagttgct
    13921 atttaattac gttacgccgg agctgacatg aaaaccaaac ttaacgaact gcttgaattc
    13981 cctactcctt ttacttacaa agttatgggg caggcgttac ctgagctggt tgatcaggtg
    14041 gttgaagtgg tacagcgcca tgcgccaggt gactacaccc caacggtaaa accaagcagc
    14101 aaaggcaact accactcggt atctatcact atcaacgcca ctcatatcga gcaggttgaa
    14161 acactgtatg aagaactggg caaaatcgat attgtccgca tggttctgta actcgcttct
    14221 ccgttacccg gctcctggtc gggtaactcc cctctcctcg ctgtgatata cttttccccc
    14281 acttttactc attctccacg gagatgccgt tttgtatcag gataaaattc ttgtccgcca
    14341 gctcggtctt cagccttacg agccaatctc ccaggctatg catgaattca ccgatacccg
    14401 cgatgatagt acccttgatg aaatctggct ggtcgagcac tatccggtat tcacccaagg
    14461 tcaggcagga aaagcggagc acattttaat gccgggtgat attccggtga tccagagcga
    14521 tcgcggtggg caggtgactt atcacgggcc gggacaacag gtgatgtatg tgttgcttaa
    14581 cctgaaacgc cgtaaactcg gtgtgcgtga actggtgacc ttgcttgagc aaacagtggt
    14641 gaataccctg gctgaactgg gtatagaagc gcatcctcgg gctgacgcgc ctggtgtcta
    14701 tgtcggggaa aagaaaattt gctcactggg tttacgaatt cgacgcggtt gttcattcca
    14761 cggtctggca ttaaacgtca atatggatct ttcaccattt ttacgtatta atccttgtgg
    14821 gtatgccgga atggaaatgg ctaaaatatc acaatggaaa cccgaagcga cgactaataa
    14881 tattgctcca cgtttactgg aaaatatttt agcgctacta aacaatccgg acttcgaata
    14941 tattaccgct taattccata cctcaatggc ccaatttaca ctgggtcatt actcataata
    15001 cctgctaatg ctttacatct ccttcgcgtt tgtatatttt ctttatcact tgcagaaatg
    15061 cggtgccacg ccttgctccg taactcaccc cattgattaa cctattgaaa tagttgatta
    15121 tggtgagcaa aagcgttccg ttccaattag acataagatt attgtaattt tttaaatatt
    15181 atttttccat gaataaaatt ggagtaagtg cgtggatagt aataatcaaa ttgaaccctg
    15241 tttaagcaga aagtcgtcag aaggtaaacc acaaatattt acaaccttac gaaatattga
    15301 tcttaacctt ctgactattt ttgaagctgt atatgtacat aaagggatcg ttaatgcagc
    15361 gaaagtgctt aatctgaccc cctcggcaat cagtcagtct attcagaaac tgcgcgttat
    15421 attccctgac ccattgttta ttcgcaaagg ccagggtgtc actcctaccg catttgcgat
    15481 gcatctacat gagtatatca gtcagggcct tgagtccatt cttggcgcgc tggatatcga
    15541 aggaagctat gataagcaac gaacgataac tattgcgacc actccctcgg tcggagccct
    15601 ggtccttcct gtcatctatc gggcgattaa aactcactat ccgcagcttt tactgcgcaa
    15661 cccgcccatc agcgacgcgg aaaaccaact cagtcagttt caaaccgatc tcatcatcga
    15721 taacatgttt tgcaccaatc gtacggtgca acatcatgtt ctgttcaccg acaatatggt
    15781 gttaatttgc cgcgagggaa atccactact ctctttagaa gatgacagag agactatcga
    15841 caacgctgcg catgtacttc tgttaccgga agagcaaaat ttcagcggtc tgcggcagag
    15901 agttcaagaa atgtttccgg accggcaaat caatttcacc agctacaaca ttttgacaat
    15961 cgctgcactg gttgccaaca gtgacatgtt agcgattatt cccagccgtt tttataacct
    16021 gtttagccgc tgctggccgc tggaaaaact gccttttccg tccttaaatg aggagcaaat
    16081 agatttctct atccactaca acaaattcag cctgcgtgat cccatcctgc acggagtaat
    16141 agatgttatc cgtaatgcat tttgaaaaag tattgttaca gccagatcac agcgcagaac
    16201 gataaaatgg cacaatccaa caacaatttt acattttcgc gaccgctttg gctgctttca
    16261 ggtccgtttc aatgatatac tgccagtcgt taattcaaaa atagttgata attacaacaa
    16321 tctattgaat tgaaacgctt tccttcgtaa ttcgcaactg gaacacgcac gctatgagta
    16381 aacccattgt gatggaacgc ggtgttaaat accgcgatgc cgataagatg gcccttatcc
    16441 cggttaaaaa cgtggcaaca gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa
    16501 tcaagcttcc agcggactct acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg
    16561 gcctgcattc tgtctgcgag gaagcctcct gccctaacct ggcggaatgc ttcaaccacg
    16621 gcacagcaac gtttatgatc ctcggcgcta tttgtacccg ccgttgtccg ttctgtgatg
    16681 ttgcccacgg tcgcccggta gctcctgatg ccaatgaacc agtgaaactg gcgcagacca
    16741 ttgccgatat ggcgctgcgt tatgtggtta tcacctccgt tgaccgtgat gacctgcgcg
    16801 atggcggtgc ccagcacttt gcggattgca ttactgccat tcgggaaaaa agcccgcaaa
    16861 tcaaaattga aactctggtg ccggatttcc gtggtcgtat ggatcgtgct ctggatattc
    16921 tgactgcaac gccaccagat gtgttcaacc ataacctgga aaacgtaccg cgtatttacc
    16981 gtcaggtacg gcctggtgca gattacaact ggtcgctgaa gctgctggaa cgctttaaag
    17041 aagcgcatcc ggaaatcccg accaagtctg gtctgatggt gggactgggt gaaaccaatg
    17101 aagaaattat tgaggtaatg cgcgacctgc gccgtcatgg tgtgacgatg ttaacgctgg
    17161 ggcaatattt gcagccaagc cgccatcacc tgccggttca acgttacgtt agcccggatg
    17221 agttcgacga aatgaaagcc gaagcgctgg cgatgggctt tacccatgct gcatgcggtc
    17281 cgtttgtccg ctcttcttac cacgccgatt tgcaggcgaa agggatggaa gttaagtaat
    17341 cctgtaataa ttacttacat ctgtctgata aaaaacccgc tttttgaagc gggttttttg
    17401 tatcgaacaa gatatagagg gagcgtcctg ctcgccacgt cactctttat gagagagctt
    17461 ttcagcctga agatcaacgt ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc
    17521 cttcttgaac cctttaatgg ccgctccaag gtctccgccc agcgtacgta acttcttagt
    17581 cccaaacagc agaacgacca gcgccgcaac taccagcagt ttggtaatac taatctcacc
    17641 catagatacc ttcttgacat ataaccggca acagaacgcg ccttaaaaac cagagaatat
    17701 taacggtcat tcgacgcgcg cacacaatag caatctgtaa caaggtgaat caagcgccga
    17761 gtaaaaaaac atcataataa ttgcggcggc gcaaagcgac ggttgtttaa gacgggcagt
    17821 tgcgcgcgca cctgacgcac acgttcgggc gtcacttccg ccataatgag tgcaggcatt
    17881 tctgacgctg ccgcaatggt gacgccaaag ggatctataa tccggctttg accgatattt
    17941 ttgttcccgc actcccccgc cgccaccata taacaggtgg tatccagcgc acgagcggca
    18001 agcaacgttg accagtgatg ctctttgagc ggcccgcgaa cccaggcggc aggaagtacc
    18061 aggatttcag ctccctgtaa tgcctgtgcc agcgccagct ctggaaagcg taagtcataa
    18121 caggtcatca gaccgacctt catcccctcc acctcgagta acggagcgat ttcattacca
    18181 gcatcaacac ggcgtgattc ctgaatggca aatgcatcat agagatgcaa tttggcataa
    18241 cgggcgacga tgtttcctgc ctgtagcgcc accagcatat tccatgcgcg ccccggcgtt
    18301 gaaggaacat gaatcgtcag aattgtcgtc atcatgttac gtttactttc tcgccgtaaa
    18361 cgtccgagga attcgccttc cagcagctgt gctgatttaa ccgatagatc tgcatcatga
    18421 tcatcgcgcg ccagcaatgc ttccggcagg acgaacagcg atacgtcgtt ttccgccgcc
    18481 tgcgccatca acgaggcaca aatctcagcg ttcttttccc acacagatgt aacagcaaac
    18541 tgtcctgcgg caaccagcat tgctacctcc tgtcagagtc gtaaaattcg ccaacgacga
    18601 ttattcggcg ttacacttat cactcataca aatcaaatag caggattttg cagtgttaca
    18661 acttctttta gcagttttta ttggcggtgg tacgggaagc gtggcgagat ggctgttaag
    18721 tatgcgattt aacccgctgc atcaggcgat tccgttgggg acgctggcag caaacctgat
    18781 tggggcattc atcataggaa tgggattcgc ctggttcagc aggatgacga acattgatcc
    18841 agtgtggaaa gtattaatca ccaccggatt ttgtggcggt ctaacaacct tctcaacatt
    18901 ttcggcagaa gtggtgtttt tgttacaaga gggccgcttt ggctgggcgt tactgaacgt
    18961 tttcgtcaac cttctggggt cttttgccat gacagcactg gctttctggc tgttttcggc
    19021 ctcaaccgca cactaaagac caaaaaaaac ccgctgatta agcgggtttt gaattcttgc
    19081 tgaagtatct tacagagcga ttacgtttgc agcagaaggg cctttggcac cgttagtgat
    19141 ttcgaactct acgcgctgac cttcagcaag agttttaaaa ccattagtct ggattgcaga
    19201 gaagtgtacg aacacgtctt tgctgccgtc ttccggagta atgaaaccga atcctttgga
    19261 ctcattaaac cacttaacgt tacctttaat cttagacatc aaaattacct ttacatgaaa
    19321 aatagacaca aatgctgtgt cggttaccag tacaccaatt gtggtacgct ttgtccagtg
    19381 caactttact aaaaagtgat aaaagtcgcg ttatttttga tgttacttat tt
//
LOCUS       DAPGWD010000070        19117 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPGWD010000070 DAPGWD010000000
VERSION     DAPGWD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.070"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(160..807)
                     /gene="ychE"
                     /locus_tag="RHX88_002528"
     CDS             complement(160..807)
                     /gene="ychE"
                     /locus_tag="RHX88_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDV2231203.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1284..3959
                     /gene="adhE"
                     /locus_tag="RHX88_002529"
                     /gene_synonym="adhC"
     CDS             1284..3959
                     /gene="adhE"
                     /locus_tag="RHX88_002529"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDV2231204.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4083..4983
                     /locus_tag="RHX88_002530"
                     /pseudo
     CDS             4083..4983
                     /locus_tag="RHX88_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5265..5882)
                     /gene="tdk"
                     /locus_tag="RHX88_002531"
     CDS             complement(5265..5882)
                     /gene="tdk"
                     /locus_tag="RHX88_002531"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDV2231205.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6488..6901
                     /gene="hns"
                     /locus_tag="RHX88_002532"
     CDS             6488..6901
                     /gene="hns"
                     /locus_tag="RHX88_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDV2231206.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7045..7953)
                     /gene="galU"
                     /locus_tag="RHX88_002533"
     CDS             complement(7045..7953)
                     /gene="galU"
                     /locus_tag="RHX88_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDV2231207.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8155..9168)
                     /gene="rssB"
                     /locus_tag="RHX88_002534"
     CDS             complement(8155..9168)
                     /gene="rssB"
                     /locus_tag="RHX88_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDV2231208.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9260..10165)
                     /gene="rssA"
                     /locus_tag="RHX88_002535"
     CDS             complement(9260..10165)
                     /gene="rssA"
                     /locus_tag="RHX88_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDV2231209.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            10278..10736
                     /locus_tag="RHX88_002536"
     CDS             10278..10736
                     /locus_tag="RHX88_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDV2231210.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10786..11628
                     /gene="purU"
                     /locus_tag="RHX88_002537"
     CDS             10786..11628
                     /gene="purU"
                     /locus_tag="RHX88_002537"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDV2231211.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11788..11872
                     /locus_tag="RHX88_002538"
     tRNA            11788..11872
                     /locus_tag="RHX88_002538"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11822..11824,aa:Tyr,seq:gta)
     gene            11920..12051
                     /locus_tag="RHX88_002539"
     ncRNA           11920..12051
                     /ncRNA_class="other"
                     /locus_tag="RHX88_002539"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12235..12912)
                     /gene="narI"
                     /locus_tag="RHX88_002540"
     CDS             complement(12235..12912)
                     /gene="narI"
                     /locus_tag="RHX88_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV2231212.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12912..13622)
                     /gene="narJ"
                     /locus_tag="RHX88_002541"
     CDS             complement(12912..13622)
                     /gene="narJ"
                     /locus_tag="RHX88_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV2231213.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13619..15157)
                     /gene="narH"
                     /locus_tag="RHX88_002542"
     CDS             complement(13619..15157)
                     /gene="narH"
                     /locus_tag="RHX88_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV2231214.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15154..18897)
                     /locus_tag="RHX88_002543"
     CDS             complement(15154..18897)
                     /locus_tag="RHX88_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDV2231215.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4831 a         5011 c         4573 g         4702 t
ORIGIN      
        1 gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg agaagaatat
       61 ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa taatgacgaa
      121 gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca ggccggggaa
      181 aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca atagccccat
      241 aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc acggtgccat
      301 gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga aaccaaacag
      361 atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg cccctggccc
      421 cgccatcaac ggcaacgcca atggcaccac accaatgctt tcacgtaccg cggtttctga
      481 tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg acatcgctat
      541 cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac caaaaagttg
      601 cagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag acaggttggc
      661 tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc tgataaagac
      721 gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac
      781 gggaaaatca aaaaaggtct gaatcacggt tagctccgaa gcaaaagccg gataatgtta
      841 gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca tttttcacct
      901 cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa aacatgctaa
      961 tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg aaaagcgatg
     1021 ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta cccagaagtg
     1081 agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc taatgtttaa
     1141 actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt tcataggtta
     1201 agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt actaaaaaag
     1261 tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt aacgcactcg
     1321 tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag caagtagaca
     1381 aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc gcgaaaatgg
     1441 ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac cactttgctt
     1501 ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg tctgaagacg
     1561 acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt atcgttccga
     1621 ccactaaccc gacttcaact gctatcttca aatcgctgat cagtctgaag acccgtaacg
     1681 ccattatctt ctccccgcac ccgcgtgcaa aagatgccac caacaaagcg gctgatatcg
     1741 ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg atcgatcaac
     1801 cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg atcctcgcga
     1861 ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct atcggtgtag
     1921 gcgcgggcaa cactccagtt gttatcgatg aaactgctga tatcaaacgt gcagttgcat
     1981 ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa cagtctgttg
     2041 ttgttgttga ctctgtttat gacgctgtac gtgaacgttt tgcaacccac ggcggctatc
     2101 tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaac ggtgcgctga
     2161 acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc ttctctgtac
     2221 cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc gaaccgttcg
     2281 cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc gaagacgcgg
     2341 tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct tgcctgtaca
     2401 ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg aaaacggctc
     2461 gtatcctgat taacacccca gcgtctcagg gtggtatcgg tgacctgtat aacttcaaac
     2521 tcgcaccttc cctgactctg ggttgtggtt cttggggtgg taactccatc tctgaaaacg
     2581 ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa aacatgttgt
     2641 ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc gcgctggatg
     2701 aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc ctgttcaaca
     2761 atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa actgaagtct
     2821 tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca gaactggcaa
     2881 actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg gacgccgcga
     2941 agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg gcgctgcgct
     3001 ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa gcgaaaatga
     3061 tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg gttgtaactg
     3121 acgacgctac tggtcagaaa tatccgctgg cagactatgc gctgactccg gatatggcga
     3181 ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc ggtggtctgg
     3241 acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag ttctctgatg
     3301 gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc taccacgaag
     3361 ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcgactatc gcgggtatcg
     3421 cgtttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg ggttcccagt
     3481 tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt cgctacaatg
     3541 cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg caggctcgcc
     3601 gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac cgtactgctg
     3661 ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg ggtattccga
     3721 aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg gataaactgt
     3781 ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg ctgatctccg
     3841 agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa ggtgaaactg
     3901 cagcgaagaa agaagctgct ccggctaaag ctgagaaaaa agcgaaaaaa tccgcttaat
     3961 cagtagcgct gtctggcaac ataaacggcc ccttctgggc aatgccgatc agttaaggat
     4021 tagttgaccg atccttaaac tgaggcacta taacggcttc cacaacaggg agccgttttc
     4081 ttatgccact tctcaatgat ctgctcgatt tcagtgacca tccgcttatg cctccgccct
     4141 ctgcacaact atttgcagaa caccttccca ccgagtggat acaacactgc ctgacgcttt
     4201 ctgctcatgc gaccgttcgc cgccgtcgtt taccggggga catggttatc tggatggtgg
     4261 tgcaatgagc caattaccga tgttgttcgc cgtctgaacc tgagcgcgga tggcgaagcg
     4321 gggatgaacc tgctggcccg cagcgctgtc acccaggcgc gtcagcgcgt gggggccgcc
     4381 ccagtggaat ggctcttccg ccagaccgca cagacagggg cgcggaacgt tacctgaagg
     4441 atgactggca cggcctgcaa ctttttgcca ttgatggcgc acagttcagg acacctgact
     4501 gataaacccg agctgcgtga atattatggg tctgccaaca catccactaa gcggcagaac
     4561 gcctacccgg ttatgcgtct ggtggcctta atgaacctgg gaagtcacat tctgctgaat
     4621 gccgtgaccg caccttaccg gcagagcgaa actgtgctgg cccactccat gctcgccacc
     4681 atcccggata actctattac gttgtttgat aaactttttt acagcgaaga cctgctgctg
     4741 acgctgaacc agaagggttg taaccgccac tggctactgc ctgcgtggaa gaatatcgca
     4801 tcagaaatga tagagctggg aaacacggcg tcaccaggga ccatcccgaa acgactggag
     4861 catcttcagg gtgctctgga agtggtgttc ataacaaaac gcccccgacc atcaaggccg
     4921 aggtcggtta agatatcaaa aacccgttat cctgtgaaac atagtgccgc tcctcttaag
     4981 tgaacggcat tgcccttctg gggccgtatt ttttgtttac ccaaagcaac ttttccataa
     5041 accgacagca ttagccttca tcatatttgc gacgatgtat aacgcctaaa cacagggata
     5101 ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga gccctgtgag gcgtatagcg
     5161 gcgttaaaaa actgccgaga agggtatata gcccggaaga agtgcgtaaa acgaactgac
     5221 aggataaaag tgccctgctc accctgtcag taaagaaatt cttattaatc gtggcgatgc
     5281 ctttcctgaa tagccgttaa tgagccgact tgtaacgcct ctttatagtg tttacggcat
     5341 acagaaacgt atcgttcatt accaccaatt accacctgct caccttcgtt ataaggtctg
     5401 cctgcttgat caagacgcag caccatgctt gctttacggc cacaaaaaca gatggttttt
     5461 aattcaacca gtttgtcgga ccatgccagt aagtattggc tgccaataaa taattcacct
     5521 cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat cgagttgatc gacaacctcc
     5581 gataattcat atacttgttg tctggttaaa aactggcatt catcaaccag tacgcaatga
     5641 attgcctgct gttcatgttc cgcacgaatc tcatcaaata atgatgaatt ttggttaaat
     5701 aattttgcag gcgatgacaa acctatacgc gaactgactt tcccggcacc aaagcgatca
     5761 tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt cctggtaatt gtatgaagat
     5821 tgcaacaatg ctgtagactt acccgcattc attgcggaat agtagaaata tagctgtgcc
     5881 atcagccaca ggccctcaat gatatgaaat aaagttggct ggagtttatc ataattcgtc
     5941 agtttttcag ctcaacagta tgcacagaag tgggtatatg cgttctccct tacgaagcct
     6001 tgcataatcc ttctgagcta tcattacaac tgcctcgctt gttataagcg ggtaaatgac
     6061 tgctggtaac tattcacaat ctttaacctg ttgcgcaagt aatagccctc tgttgacctc
     6121 caggagatag tgcaatacta agtccatgct cttattgcga ctgttctact tttcatcatt
     6181 cgcttaatag ggaattctcg taaacacaac taatacagaa gactgaaagg tcgtcagcct
     6241 acgataatct ccccataaaa tgtgacatga atcaggaagt tttaacctca cgtgctgcga
     6301 aatcatcggt gtaaataggg ctatatgccg cgtcttttct ggctaatttt atgaaaagat
     6361 atttattggc ggcacaaaat aaagaacaat tttgaattcc ttacattcct ggctattgca
     6421 caactgaatt taaggctcta ttattacctc aacaaaccac ccccaatata agtttgagat
     6481 tactacaatg agcgaagcac ttaaaattct gaacaacatc cgtactcttc gtgcgcaggc
     6541 aagagaatgt acacttgaaa cgctggaaga aatgctggaa aaattagaag ttgttgttaa
     6601 cgaacgtcgc gaagaagaaa gcgcggctgc tgctgaagtt gaagagcgca ctcgtaagct
     6661 gcagcaatat cgcgaaatgc tgatcgctga cggtattgac ccgaacgaac tgctgaatag
     6721 ccttgctgcc gttaaatctg gcaccaaagc taaacgtgct cagcgtccgg caaaatatag
     6781 ctacgttgac gaaaacggcg aaactaaaac ctggactggc caaggccgta ctccagctgt
     6841 aatcaaaaaa gcaatggatg agcaaggtaa atccctcgac gatttcctga tcaagcaata
     6901 atcttttgta gattgcactt gcttaaaatc ccgccagcgg cgggattttt tattgtccgg
     6961 tttaagacaa tttaataaga ataagctata aaaaaacggc gtcgattgct caacgccgtt
     7021 tcgtggataa caccgatacg gatgttactt cttaatgccc atctcttctt caagccaggc
     7081 tttaaattcc gtgccaaggg tgttatgacg aataccgtat tcaacgaagg cctgcatgta
     7141 acctaattta ttaccgcagt catggctctt ccctttcata tgataggctt ccaccgtttc
     7201 tttttcgatc agcatatcaa ttgcgtcggt gagctgaatt tcatcaccag ctcccggagg
     7261 ggtttttgcc agcaacggcc aaatatccgc gctaagtacg taacgaccca caatagcgag
     7321 attagacggc gcaacatccg ctttcggttt ttctaccaca ccaaccatcg gtacgctttc
     7381 acccggcgct aattcaacgc ctttgcaatc cacaacgcca tatgcggtca catcagcaac
     7441 cggttcaacc atgatctggc tatgacccgt ttcatcaaag cggcggatca tctctgccag
     7501 gttatcctgt gacaaatcgg attcatattc atccagaata acatcaggca aaataacagc
     7561 taccggttca tcacccacta ccgggtgagc acacaatacc gcgtgtccca ggcctttcgc
     7621 cagaccctga cgaacttgca taatagtcac gtgcggtgga caaatagact gcacttcatc
     7681 aagcagttga cgttttacac gtttttccag cattgcttcc agttcaaaac tggtatcaaa
     7741 gtggttttca atagagtttt tagatgagtg tgtaaccagc acaatttcag taatgccagc
     7801 cgcaatacat tcattcacga cgtattgaat taatggctta tcgacaagtg gcagcatctc
     7861 tttcgggatg gctttcgtcg ccggcaacat cctggttcct aatcccgcaa cggggataac
     7921 ggcttttttg actttcgtat taatggcagc catttaaatt ctcctggact gttcgtgttt
     7981 tgaacgtgtt catatttctg tatcgcatcc cagtatatca gcaccccgaa ggggctaagt
     8041 tctgaaaagg aggcgagtta gcataattaa gacaattact tatacgaatg gcgacgatag
     8101 taccaccagc cgacattagc aggtaatgca aatttagccc gcgttatcgt ttgctcattc
     8161 tgcagacaac atcaagcgca gtcgaccacc ggttccccat atttggcatt gccaggcatc
     8221 gcatcgctgg ctcaattgat tcaaataagc gttacctaaa gtgcctaacg gaacaccatt
     8281 actgatttgc acctggtgtt cgccggtatt taacgtcgca ttcagacccg cagaaaccag
     8341 aatgagattt ttcagttcgc gatgataata gccaactaat agcggaaact gccccggcag
     8401 attggcctga cgaagtaaat ggtttacctg cttcaataac gcgcccaact ctggcaaccg
     8461 ttgattttgg tgtgcaagct gttcctgtaa taatccgtta aacaatgcgc gtaataacaa
     8521 ggcagcaagt acgccattat gtccagctcg ggtgacatca aggcaataaa atgccagatc
     8581 gttttccgaa agtgcggcaa tatcaagcac caggccgggt ttgtccgcgg caaccaattg
     8641 acgataatta acccggcaat gggaaatcac ctgctgaacc ggcggttgta gttcctgtaa
     8701 taatttcgcc gctgcggcag ggttatcaac cattgcatcc cagtcgcgaa aaagcctttc
     8761 ctcttcctca acgcgcgaat taaacatgct gggatagaga caggcaaaaa ccatctcgcg
     8821 caagcgattc agatctttaa ctggtttcag caaaacatct tcaacgccca gacgtaacgc
     8881 tttggcaata tctgccatat tttcagtggc agatatcacc agaactgggg tctggtcgcc
     8941 tctgttacgt atatgctcca gcagtttaag cccgttcatt cgtggcatcg cgatatcaca
     9001 tatcatcagg tctggagtga aacctcccag caactcaagg gcatccaccc catcagccgc
     9061 cagtaccgtt gtcgctccca atgaggaaaa ccatgaatcc agaagcgagc gaaatacctg
     9121 ctcatcttca acaatgagaa tctgttttcc gaccaatggc tgcgtcatgt tctctcccct
     9181 gactggcttt actcaatagt ggcatgctat tgccacctgc gcctgtcaga attaacttaa
     9241 gtgtagataa aaattctggt caaatgttgg tgcgtaccaa cggcaaaagt tcgtccattt
     9301 tcctttccac tgccagctgt ccggccgcaa tggcagcgtg cgcgcgatgg aaatcaagcg
     9361 tagatatttg cgggcaaaca ggttgaatca gaatatcggg cggatcacct gccatgcggt
     9421 tccttttaag gcggttctcc agcacctgga tagaagtggt cataatctct gttgccgttg
     9481 gcgctgtcac cgcccgacgt gtcgttatgc tgcctaacct ttctttcaga cgcgcatgcc
     9541 acggcagaga atcaccattc tcgctatttt cttcactgac attaaaggag agcaaatctt
     9601 gttgcatcaa atgagcatcg tgctgcaggt caaccgctat cacaatatca gcccccaatg
     9661 cacgcgtgag ggaaatagga attgggttaa cgactgctcc atcaaccagc cagtagccgt
     9721 tatgtgcaac aggtgccatg agtcctggaa tactgcatga tgcgcgaata gcaagatgga
     9781 gatcgccttc agtaaaccat aattcacgtc ccgtacttaa attggtggca acagccgcaa
     9841 agcgacggga acaattttcg atctctgttt ccggcattat ttcgcggtat tgattgaaga
     9901 cacgctcgcc gcgcagtaac ccaccgcgct gccaggagag atccatcagg cgtaaaacat
     9961 cccaataact gaaagaggtc acccaatctt ccaacgcaga taatcgatcg catgcatagg
    10021 cagcgcccac cagcgaacca attgaacatc ctgcaacgat atcaatttca atacccactt
    10081 tttttagcgc attaataacg ccaatatgcg accaacctct cgccgcgcca gatcccaacg
    10141 ccagccctat ttttatcttt ctcattattc ccgcaagatt cccctggaat gcaatcgtag
    10201 ccacatcgct actgctcggt taacatagtg ctactcaggc ggttaatacg ccgttattgt
    10261 ttccagggag attatttgtg tctcagcttt gtccctgtgg tagtgctgtc gagtatagcc
    10321 tatgttgcca cccttatgtg tctggtgaaa aggttgcacc tgatccagaa catctcatgc
    10381 gttcgcgtta ctgcgctttt gtgatgcaag acgcagatta tttaataaag acctggcatc
    10441 cctcttgtgg agcagcggcg ttacgtgccg aattgatggc cggatttgcg catactgaat
    10501 ggttgggatt gaccgtattt gaacattgtt ggcaggacgc cgacaatatc ggatttgtca
    10561 gttttgttgc tcgatttacc gaaggtggta aaacaggtgc aataattgaa cgttcgcgat
    10621 ttttgaaaga aaacggtcaa tggtactata ttgacggtac acgtccgcag tttggtcgca
    10681 acgatccctg cccttgtggt tcaggtaaaa aatttaaaaa gtgctgcggc caataatggt
    10741 tgacggtacg gtttagcaaa cactctcaac aaggtttttc cagcaatgca ttcactccaa
    10801 cgtaaagttc tgcgtactat ttgtccggac caaaaaggtc tgatcgcacg tattaccaat
    10861 atttgctaca agcacgagtt aaatatcgta cagaacaatg aatttgttga tcaccgtacc
    10921 gggcgctttt ttatgcgcac ggaactggaa gggattttta atgattccac cctgctggcg
    10981 gatctcgata gcgcattgcc agaaggctcc gtgcgtgagc tgaatcctgc cggtcgtcgc
    11041 cggatagtga ttctggtcac taaagaagcg cattgccttg gcgatttgtt gatgaaagcc
    11101 aattacggcg gcctggatgt cgaaatcgcg gcagttattg gtaaccacga tactttacgt
    11161 tctctggttg agcgttttga tattccgttt gagctggtaa gccatgaagg gttaagccgc
    11221 aacgagcacg atcaaaagat ggcggacgcc attgatgctt atcaacctga ctatgtggtg
    11281 ctggcgaagt atatgcgggt attaacgccg gaatttgtgg cacgcttccc gaataaaatc
    11341 atcaatattc accattcatt cctgcctgcg tttatcggcg cacgtcctta tcaccaggcc
    11401 tatgaacgtg gtgtgaagat tattggcgca accgctcact atgtgaatga caatctggac
    11461 gaaggcccaa tcatcatgca ggacgttatt catgtcgatc atacctacac agctgaagat
    11521 atgatgcgcg caggtcgtga cgtcgagaaa aacgtcttaa gtcgtgcgct atacaaagta
    11581 ctggcacagc gcgtctttgt ttacggtaat cggacgatta ttctttaatc gccagcaaaa
    11641 ataactggtt acctttaatc cgttacggat gaaaattacg caaccagttc atttttctca
    11701 acgtaacact ttacagcggc gcgtcatttg atatgatgcg ccccgcttcc cgataaggga
    11761 gcaggccagt aaaaagcatt accccgtggt ggggttcccg agcggccaaa gggagcagac
    11821 tgtaaatctg ccgtcatcga cttcgaaggt tcgaatcctt cccccaccac catcactttt
    11881 caaaagtccg taaactctca aacgaatccg caattaaata ttctgcccaa gcggggaagg
    11941 atgagaagct tcgaccaagg ttcgacttga gcgccagcga gagagcgttg ccgcaggcaa
    12001 cgacccgaag ggcgaagcgc gcagcgctga gtaatccttc ccccaccacc atcgcttcct
    12061 taaataatca aaacctgtta ctccgagcta attttgcccg atggcaccac gtttaccggg
    12121 cctacaatat cctcccctgt aggtcggata agcgaagcgc atccgacaac tcaccccgca
    12181 ccgtgcccat aaaaaaaccc cgccgaagca gggtctatgt gaactaaaat tcgcttagtg
    12241 acgagcgcgc accagctggt acttacgtgt cagatactcc accggtacgc tccagatgtg
    12301 taccagacgc gagaacggga acagcaggaa taacgtcatc cccagcacca ggtgcagacg
    12361 gaagataaac gccacaccat cgaggtgttg agaagcgcca ccgtggaagg tcaccaccga
    12421 ctgcgcccag ccaaccagtt tcatcatctc gctaccgtcc atatgctgag cggagaacgg
    12481 aatggtcaac aggcccagcg cgcactggat aacgagcagc gacaggatca ggatatccgc
    12541 tccggtagtg gttgcacgca cgcgtgggct gaacagacga cgcttcagca acagcacgcc
    12601 gccaatcaga cacagcacac cgctggcacc accagcaaac atcgccattt tctgtttcac
    12661 ttcaatcggc agccaggctt catacatcca gtgcggcgtc agcataccga agaagtgacc
    12721 aacaaaaatc cccagaatac cgatatggaa caggttcgac gccaggttca tccctttgcg
    12781 atccagcatc tggctggacg ccgcgcgcca ggtgtactgc ccgtagtcat aacgcagcca
    12841 gctaccaatc aggaagaccg ccccggctat gtacgggtag atatcaaaga agaacatatt
    12901 caggaattgc attagtgctg tcctccggtg gtgatattca gatattgcgg cgcgacggca
    12961 ccggcaaagc gacgctgatg agcagtgatt gctgaatcac cgcagccttt gtcagcaaag
    13021 aatttaacct gctcttcttc ccagacagca tccagcgcct gcggcgtatc atcgcgcgct
    13081 tcgtcggcaa ttttttccgc cactttgtcg ctgtcgatag cggtattggc caatttcagc
    13141 agcagatcaa acatcacggc ataacggctt tcacgctgtt gcagacgcgc gctcagcaat
    13201 gccagaatcg gcgcgatatc tttcaaacct tccacggctt cgctttgcgg cagctgcgac
    13261 aggtactcca gatacagcgg cagatggtcc ggtaattcgc ggctgtttaa ctgcaagccg
    13321 tgctgctcgt actgcgccag caggtccacc atcgcctggc cgcggtcacg ggattcaccg
    13381 tgaacatgtt caaatagcaa cagtgacgtg gcgcggccac ggtcgaacag ttcgctgtac
    13441 tgggcctggg catcgagcgg atccatcgcc gttaaatcgc gcaggaaaat gcccagcgca
    13501 tgggcatctt ctttcgacag atttttcgac gcagcaatcg cctcaaacat ctcttgttga
    13561 tgctgccata aggcagcatc cggatattca aggagacgcg atacaatcac gagttcgatc
    13621 atggatgcgg ctccgttttg ctggtcacat cgatggcatc gatacgacgg ctgttgaaca
    13681 gattgaattt ggtatctgaa ccgtggcagc catcaccaaa ggtaaagccg cagccatttt
    13741 tctccgggaa ggcttcccgt gccagttcac gatgactact cggcaccaca aagcgatctt
    13801 cgtagttagc aatcgccaga taacggtaca tctcctgtgc ctgggcttcg gtcagaccga
    13861 cctcttccag cgcacgggta tcaactttac cgtcaacggt ttcagcacgt ttgtaatgac
    13921 gcatcgccaa catacgtttc agtgcgcgca gtaccggttt ggtatcaccg gcggtcagca
    13981 gattcgccag atactgtacc ggaatacgca agctttcgac gtctggcaga atgccgttgc
    14041 tacccagctc acccgcgtct gctgcagact gaatcggaga cagaggcggc acgtaccaga
    14101 ccatcggcag tgtgcgatat tctggatgca gcggcagcgc cagtttccat tccattgcca
    14161 ttttataaac cggcgactgc tgtgcggctt caataacgct cagcggaata ccgtctttaa
    14221 tcgcctgctc gatgactttc ggatcgttcg gatcgaggaa cacctccagc tgacgctggt
    14281 aaagatcttt ctcgttctcg gtgctggctg cacgttcaat cgcgtcggca tcgtacagca
    14341 gcacgccaag ataacggata cgaccgacac aggtttctga gcacacggtc ggctgacccg
    14401 cttcaatacg cggatagcag aagatgcact tctcagactt accgctcttc cagttgaagt
    14461 agattttttt gtacgggcat ccggtgatgc acatacgcca gccacggcat ttatcctggt
    14521 cgatcaggac gatgccatct tcttcacgct tgtaaatcgc accgctcggg caggtcgcca
    14581 cacatgccgg gttcaggcag tgttcgcaca ggcgcggcaa atacatcata aaggtgtttt
    14641 cgaactggct atacatcgcc ttctggatgt tgtcgaagtt cttgtctttc gccagtttgt
    14701 caaactcacc acccaggtca tcttcccagt tcggcccttt ttcgattttc gccatccgtt
    14761 ccccggtaat cagcgaacgc ggacgggcaa tcggctgcga tttgctgcct tccggcgcgg
    14821 tatgcaggtt ctgatagtca aaatcgaacg gctcgtaata atcgtcgatc cccggcagat
    14881 gcgggttagc gaagatttta cccagcaaca tggcacggtt acccatgcgc ggctgcagtt
    14941 tgccgttgat tttacggatc cagccgcctt tgtatttttc ctggttttcc cagtcagtcg
    15001 ggaagccctg acccggcttg gtttccacgt tgttgaacca cgcgtattcc acgccttcac
    15061 ggctggtcca gacgttttta caggtaactg aacaggtgtg gcagccgatg cacttatcga
    15121 gattcagcac catgccgact tgtgaacgaa ttttcatttt acgctctcct gtacctggtc
    15181 attgccttcg ccatctaacc agtcaatgtt cttcatctta cgcactacaa caaactcatc
    15241 gcggttagaa cctacggtgc cgtagtagtt aaagccgtat gccagatggg catagccgcc
    15301 gatcatatgc gtcggtttcg gcgtgatacg ggtgaccgag ttatggatac caccacgctg
    15361 ttgggtaatt tccgaaccag gcaggttaac gatacgttcc tgcgcgtggt acatcatggt
    15421 catccctgcc ggaacacgct ggctgacaac cgcacgggca gtcagagcac cgttgctgtt
    15481 gaagacttca atccagtcgt tatcggcgat acccagatct ttggcatcgg cttcactcaa
    15541 ccagaccacc ggaccaccgc gacctaaagt cagcatcagc aggttgtcgc tgtaggtgga
    15601 gtggataccc cacttctggt gcggcgtcag gaagttgagc gctttttccg ggttgccatt
    15661 ggatttctgg cccatcactt ctttcaccga acgggtgtcg atcggcggac gataaaccag
    15721 caggctttca ccgaaatcac gcatccattg gtgatcctga tacagctgct gacggccaga
    15781 gagcgtacgc cacgggatca gctcgtgaac gttggtgtaa ccggcgttgt aagaaacgtg
    15841 ttcatcttca aggccagacc aggtcgggct ggagatgatt ttgcgcggct gtgcctgaat
    15901 atcgcggaag cgaatcttct cgtcttcttt attcagcgcc agatgcgtat ggtcacgacc
    15961 ggtaaactcg ctcagggcag cccaggcttt cacggctacc tgaccgttgg tttccggtgc
    16021 cagcgtcagg atcatctctg ccgcatcaat tgcggtgttc agcatcggct ggcctttcgc
    16081 cggaccttcc gctttggtgt agttgagctt acgcagcaga tccatctcgc tctgggtgtt
    16141 ccaggcaatc cctttaccgc cattaccgat tttctccatc agcgggccga tagaggtaaa
    16201 gcgttcgtaa gtcgccggat aatcgcgctc tacgaccata atgtgtggcg cggttttacc
    16261 agggatcagg tcgcactcgc cttttttcca gtctttcaca tccagcggct gcgccagttc
    16321 agcggcagag tcgtgctgga taggcagcgt gacgatgtcg gtttctttac ccagatggcc
    16381 gacgcacact tcggagaatt tcttcgcgat ggctttgtag atttcccagt cgcttttcgc
    16441 ttcccaggcc ggatcgaccg ccgcagacag cgggtgaata aacggatgca tatccgaagt
    16501 attcatgtcg tctttttcgt accaggtcgc cgtcggcaaa atgatgtcgg aatagagaca
    16561 ggtgctcgac agacggaagt ccagcgtaac caccagatcc agcttgcctt ccagaccatt
    16621 gtcctgccag tccacttctt ccggcttcac gccgccctgt tgccccagat ctttaccctg
    16681 gataccgtgc tccgtcccca gcaggtactt gagcataaac tcatgacctt taccggaaga
    16741 accgagcagg ttagaacgcc agatgaacag gttgcgcggg tggtttttac cgttttctgg
    16801 ttgttctgcc gcaaaacgga tggaaccctc tttcagggat ttcaccgtat agtccaccgg
    16861 attcatcccg gctttttccg cttcgccagc gatagtcagc gggttagtgc ctaactgcgg
    16921 tgcagacggc agccagccca tgcgttccgc acggacgtta aagtcgatca agtgtccggt
    16981 atagcgggat ttgtccgcca tcggtgacag caactcttcc gccgtgacgg tttcataacg
    17041 ccactggctg gagtggttat agaaataaga agtgctgttc atgtgacgcg ccggacgctg
    17101 ccagtcaagg gcaaacgcca gcggctgcca gccggtttgc ggacgcagtt tttcctgacc
    17161 tacatagtgc gcccagccgc ccccgctctg accgacacag ccgcagaaaa tcagcatgtt
    17221 gatcagacca cgatagttca tatcgaggtg ataccagtgg ttcagccccg caccgacgat
    17281 aatcatcgaa cgaccgtgcg ttttatcagc gttatcggca aattcacggg cgatgcgaat
    17341 aatctggctg cgagaaacgc cggtaatctg ctcggcccag gccggggtat aagctttcac
    17401 atcgtcatag ctggttgcac agttaacgtc gttcaggcca cgttccagac cgtagtttgc
    17461 cagcgtcaga tcataaacgg tggtcaccag ggcggtgctg ccatcagcca gttgcaggcg
    17521 tttcaccggc agtttgtgca gcagcacgtt ttccagttcc actttgttga agtgttccgt
    17581 gccgtcgcca ccaaagtacg ggaagcctac ctcagcgatc tcatcctggc tacccagcag
    17641 gctcagttgc agctcggttt cttcgccagt tttgccgtcg cgctgttcaa gattccattt
    17701 gcccttctcg ccccagcgga agccaataga accgttcggc gcaaccattt cgccattggt
    17761 attaaaggcg acagttttcc attccggatt gttttcctgg cccagcgcat caaccagatc
    17821 agcagcgcgc agcatacgac ctgcagcgta gtaaccgtcg cgttcttcca gcatcaccag
    17881 catcggcatg tcggtgtagc gacgcacata gtcggtgaaa tactggcttg ggttgtcgag
    17941 gtggaattca cgcagcatta cgtggcccat cgccagcgcc attgccgcat cggtgccctg
    18001 tttcggtgcc agccacagat cgcacagttt ggcgatttca gcgtagtctg gtgtgacggc
    18061 aacagttttg gtccctttgt aacgcacttc agtaaagaag tgagcatccg gggtacgcgt
    18121 ctgcggcacg tttgaccccc aggcgatgat gtagctggag ttgtaccagt cagcagattc
    18181 cggtacgtca gtttgctcgc cccaggtttg cggagacgca ggaggcaagt cgcagtacca
    18241 gtcgtagaag cttaagcaag taccgccaat cagcgagaga tagcgtgcac ccgatgcgta
    18301 agaaaccatc gacattgccg gaattggcga gaaaccagca acacggtccg ggccgtagtt
    18361 tttgatggtg taaacgttag atgcggcgat cagttcgttc acctcctgcc aggaagaacg
    18421 aacaaatcca ccgcgtccac gcgcctgctt aaagcttttc gctttatcgg cgtcttcaat
    18481 gatagaagcc catgcctcaa ccggatcgct atgcagcgcc ttcgcttcac gccacatttt
    18541 catcaggcgt ttgcgcatca tcgggtattt caggcggttg gcactgtaaa gataccaaga
    18601 gtagctggca ccgcgcgggc agccgcgagg ttcatggttt ggcagatccg gacgggtacg
    18661 cggatagtca gtctgctggg tttcccaggt gaccagaccg tttttgacgt agattttcca
    18721 gctacaggag ccggtgcagt ttaccccgtg ggtagagcgg acgattttgt catgctgcca
    18781 acgctggcga tatccatcct cccagtcacg gttggtattg agaagctggc catgcccatc
    18841 ggcaaaggtt tcacccttct gtttgaagta gcgaaaccgg tccaggaatt tactcatcgg
    18901 ttttctcctg tgggagcctg tcggccctct gataaatcga cattgctaat gtttaagggc
    18961 aaggtaacgc tctgaaacag cgtgggaatt gataacgatc aagagtgatg gggatgaaaa
    19021 ataaagtaaa ttcctaagat acccctttag gataattaac agacaccaca taacagactg
    19081 taaattaaga cgttttatac ctgtagttat ttaaact
//
LOCUS       DAPGWD010000071        18953 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPGWD010000071 DAPGWD010000000
VERSION     DAPGWD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.071"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            47..1282
                     /locus_tag="RHX88_002544"
     CDS             47..1282
                     /locus_tag="RHX88_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2231216.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRSLIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            1334..2269
                     /gene="ttcA"
                     /locus_tag="RHX88_002545"
     CDS             1334..2269
                     /gene="ttcA"
                     /locus_tag="RHX88_002545"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDV2231217.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2398..3771)
                     /gene="dbpA"
                     /locus_tag="RHX88_002546"
     CDS             complement(2398..3771)
                     /gene="dbpA"
                     /locus_tag="RHX88_002546"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDV2231218.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4249..5232)
                     /gene="zntB"
                     /locus_tag="RHX88_002547"
     CDS             complement(4249..5232)
                     /gene="zntB"
                     /locus_tag="RHX88_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDV2231219.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5487..6719
                     /gene="dgcM"
                     /locus_tag="RHX88_002548"
     CDS             5487..6719
                     /gene="dgcM"
                     /locus_tag="RHX88_002548"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDV2231220.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKGLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6740..7303)
                     /gene="smrA"
                     /locus_tag="RHX88_002549"
     CDS             complement(6740..7303)
                     /gene="smrA"
                     /locus_tag="RHX88_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDV2231221.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7633..8541)
                     /locus_tag="RHX88_002550"
     CDS             complement(7633..8541)
                     /locus_tag="RHX88_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2231222.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            8717..10027
                     /gene="abgA"
                     /locus_tag="RHX88_002551"
     CDS             8717..10027
                     /gene="abgA"
                     /locus_tag="RHX88_002551"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDV2231223.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10027..11472
                     /gene="abgB"
                     /locus_tag="RHX88_002552"
     CDS             10027..11472
                     /gene="abgB"
                     /locus_tag="RHX88_002552"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDV2231224.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAECAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            11509..13035
                     /gene="abgT"
                     /locus_tag="RHX88_002553"
     CDS             11509..13035
                     /gene="abgT"
                     /locus_tag="RHX88_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDV2231225.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILDSSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13046..13561
                     /gene="ogt"
                     /locus_tag="RHX88_002554"
     CDS             13046..13561
                     /gene="ogt"
                     /locus_tag="RHX88_002554"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDV2231226.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13756..14508
                     /gene="fnr"
                     /locus_tag="RHX88_002555"
     CDS             13756..14508
                     /gene="fnr"
                     /locus_tag="RHX88_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDV2231227.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14660..15610
                     /gene="uspE"
                     /locus_tag="RHX88_002556"
     CDS             14660..15610
                     /gene="uspE"
                     /locus_tag="RHX88_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDV2231228.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(15660..15917)
                     /locus_tag="RHX88_002557"
     CDS             complement(15660..15917)
                     /locus_tag="RHX88_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDV2231229.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16161..17192
                     /gene="ynaI"
                     /locus_tag="RHX88_002558"
     CDS             16161..17192
                     /gene="ynaI"
                     /locus_tag="RHX88_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDV2231230.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(17243..18856)
                     /gene="mppA"
                     /locus_tag="RHX88_002559"
     CDS             complement(17243..18856)
                     /gene="mppA"
                     /locus_tag="RHX88_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDV2231231.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT         4899 a         4655 c         4577 g         4822 t
ORIGIN      
        1 tggtacaact atccaaagat aaatcagttt atcaaagact catgatatgt ctaaattacc
       61 aacaggtgtc gagattagag gtagatacat tcgcatctgg ttcatgtttc gaggaaaacg
      121 atgtcgggaa acattaaaag gctgggagat tacaaacagt aatattaaaa aggccggaaa
      181 tttaagatcg ctgatagttc atgaaataaa ctccggtgaa tttgagtatt taagacgttt
      241 tccccagtcc agcactgggg caaaaatggt gacaacgaga gtcataaaaa cgttcggaga
      301 gctttgtgat atctggacaa aaattaaaga gacagagtta acaacaaaca caatgaagaa
      361 aacgaaatca caattaaaaa cactcagaat aataatttgt gaaagtaccc cgatatcaca
      421 tattcgttat agcgatatct taaactaccg gaatgaactg ctgcatggag aaacgcttta
      481 cctggataat ccaagatcca acaaaaaagg aagaaccgtg cgcacagttg ataactatat
      541 cgccctgctc tgttcgctgt tgcgttttgc gtatcagtcg ggatttatat caaccaaacc
      601 atttgaagga gtaaaaaaat tacagcgaaa cagaataaag cctgatccgt tatctaaaac
      661 agaattcaat gcattaatgg aaagtgaaaa aggacagagc cagaacttgt ggaaatttgc
      721 cgtttactca ggacttcgtc acggggaact ggcagctctg gcgtgggagg atgtggatct
      781 cgaaaaggga atagtgaatg tcagaagaaa cctgacgata cttgatatgt tcggtccccc
      841 aaaaacaaat gccgggatcc gaacagtaac actactgcag cctgctcttg aagcactgaa
      901 ggagcaatac aaactgaccg ggcatcatcg caaaagcgaa atcacctttt atcatcggga
      961 gtacggcaga accgaaaagc aaaaactgca ttttgttttc atgcccaggg tgtgtaacgg
     1021 aaaacaaaaa ccttattact cggtaagcag tttgggggca aggtggaatg cagcagtaaa
     1081 acgtgctggt attcgccgcc gtaatccgta ccatacgcgg catacttttg cctgctggct
     1141 gttgacggca ggagcgaacc cggcatttat agccagccaa atggggcatg aaactgcgca
     1201 gatggtgtat gaaatttacg gtatgtggat tgatgacatg aacgacgaac agatagccat
     1261 gttgaatgcg cggttatcgt agttgcaaag tttgccccca atttgcccca tttagtacca
     1321 gagaactgaa ataatgcaag aaaatcaaca aattacaaag aaagaacaat acaacctgaa
     1381 caaattacaa aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat
     1441 tgaagaaggc gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct
     1501 ggagattctg cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa
     1561 cctcgatcaa aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct
     1621 gggcgttgag tacaagattg ttgaagagaa tacttacggt atcgtgaaag agaagattcc
     1681 agagggcaaa accacttgct cactgtgttc tcgccttcgt cgcggtatcc tttatcgtac
     1741 cgcaacggaa ctgggggcga cgaagatcgc gttgggtcac catcgtgacg atatcctgca
     1801 aacgttgttc ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat
     1861 gagcgatgat ggcaaacata tcgttattcg tccgctggcc tactgccgcg agaaagatat
     1921 tcagcgattt gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca
     1981 gcctaacctg caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc
     2041 agggcgtatc gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga
     2101 taccaacctg ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga
     2161 tctggcgttt gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga
     2221 tgaaaatcag ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg
     2281 cccgatgcgc aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc
     2341 gcatccggca atcccgcata agatgtttac tcttgcaccc ggcaattcaa catttcatta
     2401 ttttaataac cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca
     2461 tgctttatga gcaacagcct gacgcaccgc gacatagaca tgcgccggat gcacggcgat
     2521 tttgccaata tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc
     2581 cgggcgcatt ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag
     2641 cggcacaatg gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc
     2701 agaaatgata ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct
     2761 atttcctgca cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag
     2821 ctcaaagttc accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt
     2881 agcaaccagt acacgggcgc taccgttagc aaaacgtacc agagtctgat cgcgatcgcg
     2941 ttgctccaga tcgccgtgta acgacaatgc actttgccct acttcattca gcgcgtcgca
     3001 gacagcctgg caatcttttt tggtattgca aaacaccacg caagaggatg gctgatgcaa
     3061 gcttaataac cgttgcaaca gaggaatttt gcctttgctg gatgtctcat aaaattgttg
     3121 ttcaatgggt ggcaaagcat ctgttgagtc aatttcaatc gccaaaggat cgcgttgcac
     3181 tcgtccgctg attgcggcga tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg
     3241 agatgcaggc gcaaaacgga tgacatcatc aatggcatcg ctaaatccca tatccagcat
     3301 gcggtcggcc tcatccatca ccaaagtatt cagcgcatcc agtgataccg tgcctttttg
     3361 caagtgatcc agcaaacgac ccggcgttgc cacgataata tgcggcgcat gttgcaacga
     3421 atcacgctgc ataccgaacg gttgaccacc gcataacgtc aaaattttgg tatttggcag
     3481 aaaacgcgcc agccgacgca attcacctgc cacctgatcc gccagttcac gcgtaggaca
     3541 cagcactaaa gcctgggttt gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc
     3601 aaaagccgcc gttttgccgc tgccggtttt cgcctgcacg cgaacatctt ttccggcaag
     3661 gatcgccgga agcgcggcgg cctgcaccgg cgtcatggtt aaataaccca actcattaag
     3721 gttcgtgagt tgggcgggag gcaaaacatt cagggtagaa aaagcggtca caatctattc
     3781 tcgtggtcat cgacgcaaag ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc
     3841 gacaatttaa tcggttcttc atcaggtggt gggtcaggca tgggttgcgg gcgagggatc
     3901 ggatcgggca ctggaacagg atcgccagga atcggttcag ggacaggaat ttgcaaataa
     3961 ataagtgtcg tcatatttcc ctccggtcat tgggtggact cttaaagggt agacgctgat
     4021 aaataacagg caaaaaaaag ccgactcatc aaagtcggcg tcgtacgaat caattgtgct
     4081 atgcagtaat tcaaaaaagg aagtaagaca atatggagcg caacgcccat cgcttgacgt
     4141 tgcattcacc tgcaagggag atattgccct gaatgggtag agagtttatt gacttcgctc
     4201 aaactttgcg gcgtttttgt atacagacag ccggaaaaat tgcttttgtt acaaccattt
     4261 actacggtgc aaccataaag caacaccacc aataagaaca actaacagaa tacaaaaaat
     4321 tgaaaatccg aattgccacc cgccgccagg gatcccacca aggttgacgc caaataaccc
     4381 tgtcagaaag gtactgggta aaaagaccat tgccatcaat gacattgtat aggtacgacg
     4441 agctaaattt tcctgcatca cctgagcgat ttcatccgcc atcacgccag tccgtgctat
     4501 acaggcgtcg atttcgtcaa gaccgcgccc aaggcgatcg gcaatatcct gcatccgacg
     4561 gcgttggtca tcgctcatcc acggcaaacg ttcactggca agacgagcat aaacatcacg
     4621 ttgcggtgcc atatagcgac gcatcacgat taattgtttg cgcagcagag ccaggaatcc
     4681 acgcggtgga atttgctgat caaggagatt atcttcaagg tcgataattt tatcgtgcag
     4741 ctgctcgata aattcgctgg aatgatcggt caacgcatcg cacacatcca ccagccaccc
     4801 tccgcaatcg gtcggacccg tgccctcttc cagatcgctc accacatcgt ccagcgccag
     4861 cactttgcgt tgtcgggtcg aaacaattaa ccgcccgtcc atatatacac gcatggcgac
     4921 cagttgatcg gggcgttcat cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc
     4981 ttcaccgaga cggctgactc ggggccgcgt gctctcgccc gccagcgcat cacgtacgtt
     5041 attgggaagc agcggtgttg tcgccagcca ttgggcgcta tcatggtgta cataattaag
     5101 gtggagccag cagggatgcg cttcatcaat cacatctgta ttttccagcg gtttaacgcc
     5161 gcctctacca tccagcatcc aggcaaatac tgcatccggg acattaacgt ccgatccctt
     5221 aatcgcttcc acagtgcctc catcatcaac gcattatttt gtagtctagc cttctggccc
     5281 tgttacgcaa catctcatca ccccattacc ctgaaatgat taataaaatt ctgtctaaat
     5341 tgaatacaaa aagcaaaatg cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc
     5401 tatcattatc agaattatct gatcatatga catggctttt ttgcgatcgg atagcaacaa
     5461 aaattgataa aaataacggg atctcaatga ttacgcacaa cttcaatacc ctggacttac
     5521 tcaccagtcc tgtctggatc gtttcgccct ttgaggaaca gttaatttat gccaatagcg
     5581 cggcgcgact gttgatgcaa gacctcacgt ttagtcagct acgaaccggt ccctattccg
     5641 tctcctcaca aaaagaactg ccgaaatacc tctccgatct gcaaaaccaa cacgatatta
     5701 tcgaaatcct cactgttcag cgtaaagaag aggaaacagc attaagctgt cggcttgttt
     5761 tgcgaaagct gacagaagca gaaccggtga ttattttcga aggtatcgaa gcgccggcaa
     5821 cgctgggttt aaaagccagt cgctcggcaa attatcagcg caaaaaacaa ggtttttatg
     5881 cgcgcttttt tctgactaac tctgcaccaa tgttgttgat tgacccctca cgagatggcc
     5941 aaatcgtcga tgctaacctc gccgcgctca atttctatgg ttataaccat gaaacgatgt
     6001 gccagaaaca tacctgggaa attaatatgc tcgggcgtcg cgtcatgcct atcatgcatg
     6061 aaatttcgca tttacccggc ggtcataaac ctttgaattt tgttcataaa ctggcggatg
     6121 gatctactcg tcatgtgcag acctatgccg ggccgattga aatttatggc gacaagctca
     6181 tgttatgtat tgtgcatgat attactgagc aaaaagggct ggaggagcag ctggaacatg
     6241 ctgctcacca tgacgcgatg accggattac tgaatcggcg acagttttat cacattacgg
     6301 aaccaggcca aatgcagcac ctcgccatcg cccaggatta cagcttgttg ctcatcgaca
     6361 ccgatcgttt taaacacatt aacgatctct atgggcattc taaaggtgat gaggtattat
     6421 gcgccctcgc ccgcaccctt gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg
     6481 gaggcgaaga gtttgtctta ttgctaccaa gaaccccact ggataccgcg ctttcgctgg
     6541 ctgaaactat ccgtgtaagc gtggcaaaag tgagtatttc gggcttacca cgctttaccg
     6601 tcagcattgg tgtggcgcat cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg
     6661 ttgatgatgc tttgtatcgg gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag
     6721 ccgcggatgc gtctcgagat caacgactgc gcttagcgtg gcgctcccag ttttcttgct
     6781 tcgcctgcgc cgttttacgt agtgcgacgt aacacgcccc gctgccgcca tgatgcggta
     6841 gcgcagtgca atatgcctga acatcatcaa attcggtcag ccagcgcgcc acatagctgc
     6901 ggacaatatt ggcatgcgat ttatcatccc gtcctttgcc atgaataatc agcacgttac
     6961 gcaaaccatc agccatggct tgttgaatga aactgaacat cattttgcgg cactcttcca
     7021 ccggctggcg caaaagattc aggctcgcct gttgcggata tttaccactg cgcagcttat
     7081 ccagcacccc atgttgcaac ccttcccgcc gaaactccag cggctgactt agtgggatga
     7141 tgtcgagaaa tccggtggtg aggaaattat caagctgcag cgtgtcgata cgctgcggcg
     7201 cacgttggtt acgcgttgga tgccagtgga catcggtagc acgtttcagc ggctggacat
     7261 cttccatggc gtcaagaaac agcgatttgt cgtcaaggtt catgttacat cctcccgcaa
     7321 ttaagagcgc gatatgataa ccagaccggg tcggtccaac aacgtattac ccaaaattcc
     7381 agtaataagt tccaaatatt gccgatattt taagcaaaat gcttatgcat gattattcat
     7441 tcacgatatt aataatgtaa cttatatttt cgtgaaatct gtcactgaag aaaattggca
     7501 actaaaggtt aaaaccgtta taacacagtc accggcgcag aggagacaat gccggattta
     7561 agacgcggat gcactgctgt gtgtactgta gagtctggcg gatgtcgaca gactctattt
     7621 ttttatgcag ttttaacttt gcagatagcc gcattctcgc cggaattgcg tgattaatga
     7681 cgcggtcagt ggtgtctggc gactatcacg ccgctgaatc aaataatagg ccgctttcgg
     7741 taaaatttcg ctaaccggca acatcaccag cccctgtccg tgcaagggat cgcagcccat
     7801 ttcttcaggc agtatgctga gaaaatcgct ttttgccacc agactgatac aggctgagaa
     7861 cgtctcgcag actacaccga cctgtggcgt ttgcgcctga tcgtcaagca attcactcaa
     7921 ctgtttgtag tagctgccgt gtggcgtcgg cattgtccag ctgtaatcca gtaactgttt
     7981 gatcgaacgg gcaccaatgg cggggtgtcc cgggcggcaa aagatcgcga attgcttttc
     8041 cagtaatttc tcaaaagtaa attcgtggtc gtacggtccc tgataatagg tattgatggt
     8101 gaaatccaat tctccctgac gcaattcatt aatcatcgac accagttgcc cttccataat
     8161 gcgtactttt acctgcggat gctgctgatg aaaacgagat atgacagctg gcatcagact
     8221 gcgggaaata ctggccccca tgccgatatt aatctgccct gccagttgcc cttgtcgttg
     8281 gcgaatatcc tcttgggctg cgcgcagttc ttcaagaata agactggcgt gctgataaaa
     8341 actttcaccg gcatcagtta acgtcacgcc tttactacgg cgaaaaaaga gttgcgccgc
     8401 taatccttct tccagctcct gaatagattt actcagtgcc ggttgcgaca tattcaacat
     8461 tcggctcgct ccgcgaatgc tgccctgacg agccacttca acaaaagcac gaatttgatg
     8521 aatttttacc tgaaaagcca tgacgccacc gataaccgtt atttatcaga ccaaagaaac
     8581 tggcatctac tttaatgcag atgattgtgt cagggtaatt tatgaacggt taaaactgtg
     8641 aaaaatcagt tagtgataag taaaaactat cgctacgtga accgggtcac actttttact
     8701 gatgacggga aaggttatgg agtctttgaa tcaatttgtt aattcgcttg ccccaaaatt
     8761 atcgcactgg cgacgtgatt ttcatcacta tgcagagtct ggctgggtgg aattccgcac
     8821 tgccaccctt gttgcggaag aattgcagca gctcggctat tcactggcgc tgggccgcga
     8881 agtagttaat gaaagtagcc ggatgggact acctgatgaa ctcactctac aacgcgaatt
     8941 cgagcgcgct cgtcaacagg gtgcgctagc acaatggatt gcggtttttg aaggtggttt
     9001 cactggtatc gtcgccaccc tggataccgg tcgccccggt ccggtgatgg ctttccgtgt
     9061 cgatatggac gcgctggatc tcagtgaaga gcaggatgtc agccatcgcc cctaccgcga
     9121 cggttttgcg tcatgtaacg ccggaatgat gcatgcctgt ggtcatgatg gacataccgc
     9181 cattgggctt gggctggcgc atacccttaa acaattcgag tccggactac atggcgtcat
     9241 caaactgatt tttcagcctg cagaggaagg tacgcgtggc gcgcgggcga tggtcgatgc
     9301 aggtgtcgta gatgatgttg attattttac tgccgtgcac attggcactg gcgtacctgc
     9361 gggcaccgtg gtgtgcggca gtgataattt tatggcaacc accaaatttg acgcgcactt
     9421 caccggtacc gccgctcacg caggcgcaaa accagaagac ggtcacaatg ccttgttggc
     9481 ggcagcacaa gccactcttg cactgcatgc aatcgccccg cacagcgaag gagcttccag
     9541 agtaaacgtg ggcgttatgc aggcaggaag cggtcgtaac gttgttcctg cctcggcgtt
     9601 gctgaaagtg gaaacacgcg gggccagcga cgtcattaat caatatgttt ttgaccgtgc
     9661 acaacaagcg attcagggcg cagcaaccat gtatggtgtc ggcgttgaaa ctcgtctgat
     9721 gggtgcagct accgccagtt ctccttcgcc gcaatgggtc gcatggttgc aaagtcaggc
     9781 ggctcaggtc gcgggggtca atcaggccat tgaacgtgtt gaagcgcctg cgggttccga
     9841 agatgccaca ttaatgatgg cccgcgtgca gcaacatcaa gggcaagcct cctacgtggt
     9901 gtttggcaca cagctggcgg caggtcatca caacgaaaaa ttcgattttg acgagcaggt
     9961 tctcgctatt gccgtcgaaa cgctggcgcg caccgcgctc aattttccct ggacgcgagg
    10021 tatctgatgc aggaaatcta tcgttttatc gacgatgcga ttgaagccga tcgccaacgt
    10081 tataccgata ttgccgatca aatctgggat catccagaaa cacgttttga agagttctgg
    10141 tcagcggagc atctggcttc ggcgctggaa tctgcaggct tcaccgttac ccgcaacgta
    10201 ggcaatatcc caaatgcctt tattgcttcg tttggtcaag gcaaaccggt tatcgccctg
    10261 ctgggagaat atgacgccct ggcaggttta agtcagcaag caggttgcgc gcaacctaca
    10321 tccgtgacgc ccggtgaaaa tggtcacggt tgcggacaca atttgctggg aaccgccgcc
    10381 tttgccgctg caatagccgt caagaaatgg ctggaacaat atgggcaagg cggcacagtg
    10441 cgcttttatg gttgtcctgg cgaagaaggc ggctcgggta aaacgttcat ggtccgcgag
    10501 ggggtatttg atgatgtgga tgcggcactc acctggcacc cggaagcctt tgccggtatg
    10561 ttcaatacac gcacgctggc aaacattcag gcatcatggc gctttaaagg gatcgcagca
    10621 catgccgcga attcccctca tttgggacgc agcgcccttg atgccgtaac gttgatgacc
    10681 actggcacca acttcctcaa cgaacatatt attgaaaaag cgcgcgtaca ctatgccatc
    10741 acagatagcg gcgggatctc gcccaacgtg gtccaggcgc aggcagaagt gctttatctt
    10801 atccgtgccc ccgaaatgac cgacgtgcag catatttatg atcgggtcgc caaaatcgcc
    10861 gaatgtgcgg cattgatgac cgaaaccacg gttgaatgcc gcttcgacaa agcctgttcc
    10921 agttatctcc cgaatcgcac cttagaaaat gccatgtacc atgccctatc ccattttggt
    10981 accccggaat ggaactccga agaactggct tttgcgaaac aaattcaggc tacgctcacc
    11041 cccaacgatc ggcaaaacag tctgaataat atcgccgcaa ccggtggcga aaacggcaag
    11101 gtttttgcac tacgtcatcg tgaaacggta ctggcgaatg aggtcgctcc atatgccgcc
    11161 accgataacg tgcttgcggc atcgactgat gtcggcgacg tcagttggaa actgcctgtt
    11221 gcccagtgtt tcagcccctg ttttgccgtc ggtacaccgc tacatacgtg gcaactggtt
    11281 agccaggggc gaacatctat tgctcataaa ggaatgctgc tggcggcgaa aactatggca
    11341 gcaaccacag tcaatctctt cattgattca gggctattgc aagaatgcca acaagagcat
    11401 cagcaagtta cggacacgca accgtatcac tgccctatcc cgaaaaacgt gacaccgtca
    11461 cctttaaaat aacaacaaca acgcaaacac aacaaccgag gaatgcccat gagtatgtca
    11521 tccataccgt cgtcctccca atccgggaag ctctatggct gggtcgaaag aattggtaac
    11581 aaggttcccc atcctttttt gctctttatc tatttgataa tcgtactcat ggtgacgacg
    11641 gcaattttgt cagcctttgg cgtcagtgcg aaaaacccga ccgatggtac gccggtggtg
    11701 gtgaaaaacc tgctcagtgt ggaaggatta cactggtttt tacccaatgt gattaaaaac
    11761 tttagcggtt ttgctccact tggagcgatc ctggcgctgg ttttaggtgc cggcctagcg
    11821 gagcgcgtcg gcttactgcc agcgctaatg gttaaaatgg catcgcatgt taatgcccgc
    11881 tacgccagtt atatggtgct gtttattgct tttttcagcc acatttcttc cgatgcggcg
    11941 ttagtgatca tgccaccgat gggtgcgctg atttttctgg cggtgggcag gcatccagtt
    12001 gcaggtttac tggctgccat tgcaggcgta ggttgcggct ttacggctaa tttactgatt
    12061 gtcacaaccg acgtgttgct gtcggggatc agcacggaag cggcggctgc gttcaatccg
    12121 caaatgcacg tcagtgtaat tgataactgg tattttatgg ccagctccgt agtcgtactg
    12181 acgattgttg gcggcctgat aaccgacaaa atcatcgagc cacgattagg tcaatggcag
    12241 ggaaacagcg atgagaaact gcagacattg accgaaagtc agcgttttgg tttacgcata
    12301 gcaggtgtcg tatcgctact ttttattgct gcgatcgcgc tgatggtgat cccggaaaac
    12361 gggatattgc gcgatccgat taatcacacc gtgatgccat caccctttat taaaggtatc
    12421 gtgccactga tcattctttt tttctttgtt gtctcgctgg cttatggcat cgctacccgc
    12481 acaattcgac gtcaggcgga tttaccgcat ttaatgattg aaccgatgaa agagatggcg
    12541 ggatttatcg tgatggtttt tcccctcgcc caatttgtcg ccatgtttaa ctggagcaac
    12601 atggggaaat tcatcgccgt ggggctgacc gatatacttg acagttcagg gcttagcggc
    12661 atcccggcgt ttgtcggtct ggcgttgctt tcctctttct tatgcatgtt tattgccagc
    12721 ggttccgcaa tctggtcgat tctggccccc attttcgtac caatgtttat gctacttggc
    12781 tttcacccgg catttgcgca aatcctcttt cgtattgccg actcatccgt attgccttta
    12841 gcgccggtat ctccttttgt tccactgttt cttggattcc tgcaacgcta caaaccagac
    12901 gcaaaactgg gtacttacta ttcgttagtc ttgccctatc cgcttatctt tttggtggta
    12961 tggctgctga tgttgctggc gtggtatctt gttggtctgc cgataggtcc ggggatttac
    13021 ccacgtttgt cttaagagag aacggatgct gagattactt gaagaaaaaa ttgccacgcc
    13081 actgggtcca ctgtgggtga tttgcgatga gcaatttcgc ctgcgggcgg ttgaatggga
    13141 agagtacagc gaacgcatgg tgcagctgct ggacatccat tatcgcaaag aaggctatga
    13201 gcgcatttct gccaccaacc caggcggttt aagcgacaag cttcgtgatt attttgccgg
    13261 taatcttagc attattgata cgcttcccac tgccacaggg gggacgccat ttcagcgcga
    13321 agtctggaaa actctacgca ctatcccctg cgggcaggta atgcattacg gccaactggc
    13381 tgaacaattg ggccgccctg gcgcggcgcg tgccgttggt gcggcaaacg gatcgaatcc
    13441 catcagcatc gtcgtacctt gccatcgggt tattggccga aacggcacca tgaccggata
    13501 tgcaggcgga gttcagcgaa aagagtggtt attgcgccac gaaggttatc ttttgctgta
    13561 aacattaaac aatttgtgcc agcttgttca cacttttatg taaagttacc cttaacaact
    13621 taagggtttt caaatagata gacatatatt tacatctgat atcggaattc tctgctgtta
    13681 aggtttgctt agacttactt gctccctaaa aagatgttaa aattgacaaa tatcaattac
    13741 ggcttgagca gacctatgat cccggaaaag cgaattatac ggcgcattca gtctggcggt
    13801 tgtgctatcc attgccagga ttgcagcatc agccagcttt gcatcccgtt cacactcaac
    13861 gaacatgagc ttgatcagct tgataatatc attgagcgga agaagcctat tcagaaaggc
    13921 cagacgctgt ttaaggctgg tgatgaactt aaatcgcttt atgccatccg ctccggtacg
    13981 attaaaagtt ataccatcac tgagcaaggc gacgagcaaa tcactggttt ccatttagct
    14041 ggcgacctgg tgggatttga cgccatcggc agcggccatc acccgagctt cgctcaggcg
    14101 ctggaaacct cgatggtatg tgaaatcccg ttcgaaacgc tggacgattt gtccggtaaa
    14161 atgccgaatc tgcgtcagca gatgatgcgt ctgatgagcg gtgaaatcaa aggcgatcag
    14221 gacatgatcc tgctgttgtc gaagaaaaat gccgaggaac gtctggctgc attcatctac
    14281 aacctgtccc gtcgttttgc ccaacgcggc ttctcccctc gtgaattccg cctgacgatg
    14341 actcgtggcg atatcggtaa ctatctgggc ctgacggtag aaaccatcag ccgtctgctg
    14401 ggtcgcttcc agaaaagcgg tatgctggca gtcaaaggta aatacatcac catcgaaaat
    14461 aacgatgcgc tggcccagct tgctggtcat acgcgtaatg ttgcctgatt tttccgcata
    14521 actcactatc cttctgtcat atcattaaat ttttctgatt tattgatctg gcagaaggtt
    14581 catcactgtt tcattcacca gatatgggtt aatcttttaa ttacaaactg cgttgacagt
    14641 tgttgtaagg agaccctgta tggctatgta tcagaacatg ctcgttgtta tcgatcctaa
    14701 ccaggacgac caaccagcat tgcggcgagc tgtttattta catcaacgga ttggtggcaa
    14761 aattaaagcc tttttgccga tctatgactt ctcatacgaa atgaccaccc tgctctcccc
    14821 ggacgaacgt acggctatgc gtcagggcgt catcagccag cgtacagcct ggatccacga
    14881 gcaggcaaaa tattatctca atgctggcgt tcccattgaa attaaagtgg tctggcataa
    14941 ccgtcctttc gaagccatca ttcaggaagt gatcagcggc gggcacgatt tggtgctaaa
    15001 aatggcgcac caacatgacc gtctggaagc ggtgattttt acgccaacgg actggcatct
    15061 gttacgcaaa tgcccaagcc cggtgtggat ggtgaaagac cagccgtggc cggaaggagg
    15121 caaggcgctg gtggcggtga atctcgccag tgaagagccg tatcataatg cgctcaatga
    15181 aaaactggtc aaagagacga tcgaactggc agaacaagtc aaccataccg aagttcatct
    15241 ggttggcgct tatccggtaa cgccaattaa tatcgcgatt gaactgccgg aatttgatcc
    15301 gagcgtttat aacgatgcca ttcgtgggca acatttgctg gcaatgaaag ctctgcggca
    15361 gaaattcggc attaatgaaa acatgacgca cgtagaaaaa ggtctgccag aagaggtgat
    15421 tcctgatttg gcggaacact tacaggcggg gattgtggtt ctgggcacgg taggacgcac
    15481 cggtatttca gcagcattcc tcggcaacac ggcggaacag gtgattgatc atcttcgctg
    15541 cgatctgttg gtcattaaac ctgaccagta tcagacaccc gttgaactgg atgacgaaga
    15601 agacgattaa cgactcttca ataactgaca aggcccgagt tatcgggcct attttttact
    15661 tactgctctt cgccgccaag gaaataaatt cccaacggga ttgccagcaa cacagtaaag
    15721 accaggctat aaacaaaaat catcgatgac tgaatcacat acattgatgt cgtccactca
    15781 gacagcggaa tattgtactg ttctataacg ccgccgatag tcgcgcgagt cacaaccgac
    15841 gccgcaataa tgaaaaccgc caacaaacca aagagaaatt tcttcccttt cgctgacttc
    15901 agtttcgcca taatcatgat gtaatccttg tgcagacaaa tttcttgtta cctaaacgta
    15961 acaataccat gacatttcac cgttgtctgc tctgaaaacc tcacctctta gctggattta
    16021 caatcaacgc attgattagt tagctaatgt tgctacttcc ttccctgact attgatgatc
    16081 tgaatgaatt gtcgcggtaa aatagccccg ccaggggttt ccggcatgac gacaattatc
    16141 tggaggagcc tgataaaaca atgatcgctg aactgtttac aaataatgcg cttaatctgg
    16201 tcattatttt cggtagctgc gcagcattga ttctgatgag cttttggttt cgccgcggaa
    16261 atcgtaaaag aaaaggtttt ttattccatg cggtgcaatt tttaatctac accataatta
    16321 tcagtgctgt tggtagcatt attaattatg ttatagaaaa ctataaactc aaatttatca
    16381 ccccaggcgt tatcgatttt atctgtacgt ccctgattgc ggttattctg acgattaagc
    16441 tatttttgct gattaatcag tttgaaaaac agcagattaa aaaaggtcgc gatatcacca
    16501 gtgcgcggat tatgtcgcgc attatcaaaa tcaccattat tgtggtgctt gttctgcttt
    16561 atggcgaaca tttcggcatg agcctttctg gcttgctgac ctttggtggt attggtggtc
    16621 tggctgtcgg tatggccggt aaagatattc tgagtaattt cttttccggg atcatgctct
    16681 atttcgaccg tcctttcagt attggcgact ggatccgttc accggacaga aatatcgaag
    16741 gtacagtagc ggaaattggc tggcgaatta ccaaaattac gacctttgat aatcgtccat
    16801 tgtacgtacc gaactcgctg ttttcatcga tcagcgtaga aaacccagga cgaatgacca
    16861 accgccgcat taccacgacc attggtttac gttatgagga tgcggcaaaa gtgggcgtta
    16921 ttgtcgaagc cgtacgtgag atgctgaaaa atcacccggc catcgaccag cgacaaacct
    16981 tactggttta tttcaaccag tttgccgact cgtcattgaa tattatggtt tattgcttta
    17041 ccaaaaccac ggtatgggct gagtggcttg ctgcacagca agacgtttat ttgaagatta
    17101 tcgatattgt acagtcacac ggcgcggact ttgccttccc cagccagacg ctgtatatgg
    17161 ataacattac accgccggaa cagggtcgct aaatcagaca tcactggcgt tatccacaag
    17221 ggtaacgcca gttcccatca catcaatgct tcacaatata catagtccgg ctgtacgcca
    17281 catcttccgg gttattaatg ggataacctt tcagccacgg cttgattaat cgtccattgg
    17341 tatattgata aattggtgca atcggtgctt gctccatgag gattttttct gccgcgttgt
    17401 aatcggcatt acgcgcttta acggtatttt ccgtcgatgc ctgggccaga actttgtcgt
    17461 atgccggatt gttaaagcgt gaaatatttc ctgaatgcgt tgacgttaat aatgtcagga
    17521 aagtggaggg ttcattataa tcccccaccc atgaggcacg gataacatca aaattgccgg
    17581 tgttacggct atcgatatag gttttccatt cctgattttg caatttaaca tctacgccaa
    17641 ggttcttttt ccacatcgat gctacagcaa tcgcaatttt ttgatggttt tctgaagtgt
    17701 tatacaaaag cgtcagcttc agcggttttt gcggaccata accagctgcg ctcaacaaag
    17761 tttttgcctg tgcattcagt tcttcctgac tcatttgttc aaacggcgaa ggttccggcg
    17821 taaatccggc ggtaacatct ggtgtaaaat gccacgctgg cttttcgccc gttcccaaca
    17881 ctttttcggt catcaggcgg cgatctatcg tcatacttaa tgccaggcga acgcgctgat
    17941 ctgccgtcgg ccctttttgc gtgttaaacg cataataata ggtcccgagc tgtggcggcg
    18001 tataaacctg ccccggaata tccttcaaca gcttctgata catatttttc gggaaggatt
    18061 cggtgatatc aatatccccc gcgaggtaac gcttagtggc tgcggattcc tgattaattg
    18121 gcaggaaggt cactttttgc agtaccgttt tggcgttatc ccaataatgg gtattcggta
    18181 ccacgaccag tttttcattg actacgcgat ctttaagaac ataagcgcca ttgccgatca
    18241 gatttccggg tttcgtccac tctttaccgc tttctacgtt ggctttttgc accgggaaga
    18301 aggcaaagtt agcggttaaa ttcacaaacc acggcaacgg tttatcaagc tgaattttca
    18361 aggtatgggc atcaactgcg gtgacgccaa gctggtcagg cgtagcttta ccatcaataa
    18421 tcgcctgtgc gttgttgatt cccgccagcg cggcaaacca tgcaaatggc gatagcgttt
    18481 tcgggtccac cagacgctgc cagctgtaga caaaatcttg cgccgttacc ggtgtgccat
    18541 ccgcccattt tgcgttatcg cgcagggtaa aagtccagat acggttgtca ttacttttcc
    18601 actgagtcgc aacgccgggg acaatctccc ctttctcgtt ctgattcacc agtccttcaa
    18661 acagatcgcg aatgacctga atctctggca ggcccacggc tttagcggga tccagcgacg
    18721 caggctcatc tttaatgtgg cgcaccagct cctgcttctc tgccagtact gtgccgctcg
    18781 gaacttctgc agcatacgaa agagaaatgc tgctgaccaa cagcgcacaa cacgtgactg
    18841 aaacagagtg cttcataagt ttccctctaa ccgacattaa aattgatgcg taattatttg
    18901 tttcgcctta aagaaatgca aacaacttcc atcaaaaaag tgacacagga agc
//
LOCUS       DAPGWD010000072        18711 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPGWD010000072 DAPGWD010000000
VERSION     DAPGWD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.072"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..776
                     /locus_tag="RHX88_002560"
     CDS             <1..776
                     /locus_tag="RHX88_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV2231232.1"
                     /translation="GFKVKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSET
                     LEFWPRKPEPGKDTFQYHVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGD
                     HGANTFKANMEEAPEQVIALLNKFAVFPSDYISDCANNRSGKSSAKLIWAAELSWMIS
                     ISSTAFQNGTIEEELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLT
                     DAELEACYRYDKQFWEHYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(893..2236)
                     /gene="gdhA"
                     /locus_tag="RHX88_002561"
     CDS             complement(893..2236)
                     /gene="gdhA"
                     /locus_tag="RHX88_002561"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDV2231233.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2472..2744
                     /locus_tag="RHX88_002562"
     CDS             2472..2744
                     /locus_tag="RHX88_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDV2231234.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(2710..3117)
                     /gene="nudG"
                     /locus_tag="RHX88_002563"
     CDS             complement(2710..3117)
                     /gene="nudG"
                     /locus_tag="RHX88_002563"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDV2231235.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            3204..3824
                     /locus_tag="RHX88_002564"
     CDS             3204..3824
                     /locus_tag="RHX88_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV2231236.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3833..5140)
                     /gene="ynjE"
                     /locus_tag="RHX88_002565"
     CDS             complement(3833..5140)
                     /gene="ynjE"
                     /locus_tag="RHX88_002565"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDV2231237.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLYEWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(5207..5860)
                     /locus_tag="RHX88_002566"
     CDS             complement(5207..5860)
                     /locus_tag="RHX88_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV2231238.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWFNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5860..7395)
                     /locus_tag="RHX88_002567"
     CDS             complement(5860..7395)
                     /locus_tag="RHX88_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2231239.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(7368..8534)
                     /locus_tag="RHX88_002568"
     CDS             complement(7368..8534)
                     /locus_tag="RHX88_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2231240.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFSPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(8544..9092)
                     /locus_tag="RHX88_002569"
     CDS             complement(8544..9092)
                     /locus_tag="RHX88_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDV2231241.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSNDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(9092..9799)
                     /locus_tag="RHX88_002570"
     CDS             complement(9092..9799)
                     /locus_tag="RHX88_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV2231242.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9814..10491)
                     /gene="ydjY"
                     /locus_tag="RHX88_002571"
     CDS             complement(9814..10491)
                     /gene="ydjY"
                     /locus_tag="RHX88_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDV2231243.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAGKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(10496..11206)
                     /locus_tag="RHX88_002572"
     CDS             complement(10496..11206)
                     /locus_tag="RHX88_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV2231244.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSSSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(11373..12179)
                     /gene="xthA"
                     /locus_tag="RHX88_002573"
     CDS             complement(11373..12179)
                     /gene="xthA"
                     /locus_tag="RHX88_002573"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDV2231245.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12625..13845
                     /gene="astC"
                     /locus_tag="RHX88_002574"
     CDS             12625..13845
                     /gene="astC"
                     /locus_tag="RHX88_002574"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDV2231246.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            13842..14876
                     /gene="astA"
                     /locus_tag="RHX88_002575"
     CDS             13842..14876
                     /gene="astA"
                     /locus_tag="RHX88_002575"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDV2231247.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            14873..16351
                     /gene="astD"
                     /locus_tag="RHX88_002576"
     CDS             14873..16351
                     /gene="astD"
                     /locus_tag="RHX88_002576"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDV2231248.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHCPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            16348..17691
                     /gene="astB"
                     /locus_tag="RHX88_002577"
     CDS             16348..17691
                     /gene="astB"
                     /locus_tag="RHX88_002577"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDV2231249.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            17684..18652
                     /gene="astE"
                     /locus_tag="RHX88_002578"
     CDS             17684..18652
                     /gene="astE"
                     /locus_tag="RHX88_002578"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDV2231250.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         4412 a         4850 c         5056 g         4393 t
ORIGIN      
        1 ccggttttaa agtaaaaaaa gaacaactgc attattatca ggctaaaggt attgagccgc
       61 tcagtatcga gaagttacag gcgctacaat tgatcgcacc ttatcgattc tatcataagc
      121 aatggtctga aacgctggag ttctggccgc gaaagcctga acctggcaaa gataccttcc
      181 aatatcatgt gcttcctttt gactcgatcg atatcataag taaaagacgc gagtctttag
      241 aggatcaatg gggtatcgaa gatagcgaaa gttattgtgc cttaatggag cattttcttt
      301 ctggcgacca tggagccaat acctttaaag caaacatgga ggaagcccca gagcaggtta
      361 tcgccttgtt gaataaattt gctgtttttc cctcagacta tatctctgat tgcgctaata
      421 atcgctccgg taaatcctcg gcgaagctaa tatgggcggc ggaattatca tggatgatct
      481 cgatatcaag cacagctttt caaaacggga caattgaaga agaactggcc tggcattata
      541 taatgcttgc ttctcgaaag gcgcacgagt tgttcgaaag cgaagaagat tatcaaaaaa
      601 atagtctaat gggatttctt tactggcata tctgctgcta tcgcagaaag ttaacggatg
      661 ctgaacttga agcgtgttat cgttacgaca agcagttttg ggagcactac agtaagaaat
      721 gccgttggcc cataagaaat gttccctggg gagcatcatc cgttaaatac tcataaacgc
      781 ctgaaatttt gccgggggcg ctgcgcttgc ccggcctaca ttagagcgta actgcaatga
      841 attgtgccca tttgtaggcc tgataagcgt agcgccatca ggcatttaca acttaaatca
      901 caccctgcgc cagcatcgca tcggcaacct tcacaaaacc ggcaatgttc gcgccctgca
      961 cgtagttggt ttgctcacct tcaccaccat gctcaacaca ggcatggtgg atatccagca
     1021 tgatgtgatg caaacgtgcg tcaactttct cggctttcca gcccaggcgc gcagcgtttt
     1081 gtgccatttc caggcccgac gttgctacac cgccagcatt agccgcttta cccggtgcaa
     1141 acagtacgcc tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc atatttgccc
     1201 cttcagcgac ggctttaacg ccattggcga taagctgatg cgcggcgtca acatccagtt
     1261 cgttctgggt ggcgcaaggc agggcgatat caaccggtac agaccacggc tgttggcctt
     1321 cgagatagac cagaccaaat tctttggcgt aatctgccac tcgaccatcg cggctggctt
     1381 tgatttcgat aagacgtgcc agtttctctt tcgtgaatcc gctttcatca actacagtgc
     1441 cgctggagtc tgacgcagtg atcacacgag caccaaattc catcgctttt tcgatagcgt
     1501 actgggcgac gttgccggag ccagaaacgg aaacgcgcat cccttcaaaa cccataccgt
     1561 ggcgttttaa cattgcttct gtgaaataaa ccagaccgta gccggtagct tccgggcgaa
     1621 taagactacc gccaaatgaa aggcccttac cggtgaagac gcaggcggta ttgttggaga
     1681 gctttttcat catccccgcc ataaagccga cttcacgacc accaacgccg atatcacctg
     1741 ccggaacgtc ggtatccgcg cccagatgac gataaagctc ggtcatcagc gcctggcaga
     1801 aacgcatcac ttcgccttcg cttttacctt tcgggtcaaa atcgctaccg cctttaccac
     1861 cgcccatcgg cagggtagtc agggcatttt tgaaggtttg ttcgaagccg aggaatttga
     1921 gaatggagag gttaactgac ggatggaagc gcataccgcc tttgtacggg ccgatggctg
     1981 agctgaactg cacacgccat gcacggttga cctgtacctg attgcgatca tcaacccata
     2041 ccacgcgaaa ctggatcacg cgctccggtt caaccagacg ctccagtaat gacatctggc
     2101 gatattttgg attttgttca agaaaaggcc agagtgtggt cattacttca cgaacggctt
     2161 gcgcgaactc ggtttgattc gggtcgcgct tttggacatg gttgaggaat gactccagag
     2221 aatatgtctg atccatagat ataaaaccct tatatattaa tacgattgtg cttatgttgt
     2281 ttatgtgatg tgcttttgca gttttcgact ataccatcaa gaatgtgacg gtaagcaaga
     2341 aaaaaatgac ccaggaaagc aaattaataa cgagagtaat ctcataacca aatgcgataa
     2401 ctcagacgct aaagttttgc cacccggcta ccgttatcag tagcagagac aataacgtta
     2461 aggagttccc tgtgagtcga gcattgttcg ccgtagtatt agcatttcca ttaattgcac
     2521 tggctaaccc gcattatcgt ccggacgtgg aggtaaacgt accgccagaa gtgttcagtt
     2581 caggcgggca gtcagcgcag ccctgtactc agtgctgcgt ttatcaggat aaaaattatt
     2641 ctgaaggcgc ggtaattaaa gccgagggaa tactgctgca atgccagcgg gatgacaaaa
     2701 cccttagcac taatccgctg gtctggcggc gcgtaaagcc ataaacgcct ctaataatgg
     2761 aatgtcagca ggggccagcg gatattgcag cgcctcttca ggtgagcacc agaccagcgc
     2821 ctgatgttca tgtgcctgta acgtcccgtg gaagtcgggt acgtgccagg catgaagatg
     2881 gataatccgg cccgaaactt ctcgctgatg gctggcaaca tactcaccca cagttgcttc
     2941 gatgcccaat tcttcgttta actcacgcac cagtgcctgc tgctggcttt catccagctc
     3001 gactttacca ccggcaaact cccataatcc cgcctgatcg ctctgggcgg ggcgttgcgc
     3061 gagtaaaatt ttgccatcac gttcaatgat ggcggcaaca acttcaatca ttttcataaa
     3121 ttacacacgg gcagataagc gaaaaatgcc ataatatccg cactgttgct caatcagaaa
     3181 ggctttatat ttaaggagtg cctgtgctag accgccatct tcatccccgg attaaaccgt
     3241 tgttgcatca gtgcgtgcgg gttcttgata aaccgggcat tacgccagat ggtttaacat
     3301 tagtgggatt tgccatcggc gtgctggctc tgccgtttct ggcgctgggc tggtatctgg
     3361 cggcgctagt cgtcattttg ttgaacaggc tgcttgatgg tctggacggt gcgctggcgc
     3421 ggcgcagaga gcttaccgac gcgggcggct ttcttgatat ttctctcgat tttctctttt
     3481 acgcgctggt gccgtttggc tttattcttg ctgcaccgga gcaaaatgcg ctggcaggcg
     3541 gctggttgtt gtttgcgttt atcggcacgg gcagcagttt tctcgccttt gctgcactgg
     3601 cagcaaaaca tcagattgat aaccccggtt atgcacataa gtcgttttac tatctggggg
     3661 gattaaccga aggcacagaa acgatcttac tgtttgtgct gggatgttta ttccctgcat
     3721 ggtttgcgtg gtttgcgtgg atcttcggtg cgttgtgctg gatgacgacc tttacgcggg
     3781 tgtggagtgg gtatctgacg ctgaagtcgc tccagcgtca gtaattgcaa tgttatttgc
     3841 tactgtccgg gccgcgttca ccggttgcta ccggattttt tggatcgctg ctccattcgt
     3901 accagccgcc gtcatacacg gaaacattct tccaacccat ggcgcgtgcg tacataaagg
     3961 tttcggacgc gcgccagccg gtgccgcagt agaatgaaac ttgctgctct ggtttgatat
     4021 tccacgcttt ccacatagcg gtaatatcat cggcgctgcg catggtgcca tccgggttat
     4081 gaaaatcttc catatgcgtc gagtcgctac cagcgtgtcc ccaacgtgct ccggctattt
     4141 caccttttgg tttaatatag ctgtaaccgc tggtcgtacc gataaattct ggccacgaac
     4201 gaatgctcac cagcgatgca tcctggcgat gcagcagtcc acgggcctgt tccatatcga
     4261 gcatcaactg cggttgtgcc gggatcttca cgccgaaatc cggttccgct ttcactttcg
     4321 gtggcgttcc tcgctcaaca ggcagtcccg cgtcggacca ggtttgccag ccgccatcca
     4381 gcagacgcac atctttcacg cctgcgtaga gcataatttg cgccacacgc gctccagcgt
     4441 atacgtcacg cccgtacaga atgaccgtgg tgtcatggcg aatgccgtgt tttgccagca
     4501 tcgcttttag ttgttcatca gaaactttgt tccacagcgg ttcgctttcc acttcgttgg
     4561 tatcgatgta gtcagcgcca ggaatatggc tgataaggta aagcttagga gcgccccagg
     4621 ccgcttcaat gactttccag tcaccggcag gtttcgccgt aacctcttta ccttgttgca
     4681 ggtcgtgcag ccattgcgga taaaccagct gctcaaagtg cggcagtttt tgtagacggg
     4741 aaggttcgct tagcgcgtca ctcagaatgg agatatgcgt aaaacctgct ttttgcagac
     4801 gcgttttgac ggcgtcgaca tctttgtcat tgccgtacag cgccaccgga gcatcggttt
     4861 tcagattatg ttgcttgatc cactcataga gctgttcggt gctcattttg tcgagccagc
     4921 tggcagagag gtttaaggcg gcaggttcat gaccagaagg gccatttaag gtttgtggcc
     4981 agccgttata aaacgcgctg gggcgagtat ctatcgcttt gccattttgt tgttgaagct
     5041 ggtcaagtgt aagaggcttc gccagttcag cggcccacga agaagcgcaa gccagcccta
     5101 aagccattgc cagcgcggtc atttgagaaa cacgtttcat cgaaaatccc gacattgtag
     5161 ttttgagtgc gccattctga actgacgcat tgagaaaaac tttgcgttat cgcagtttgt
     5221 tgtaattttc tgaccactgc gccatatcca gaacagaact atcagcagga acatcctgga
     5281 gatcgtgcgt tacctgaacg acggggatcg ccagttcgcg aacttcgctg aacacccact
     5341 ggcgaaaatt atcgcgcaga gccacatcaa gacggctgaa tggctcatcc aggagtaacg
     5401 cttttggctg agcgagaagg gcgcgcagca gggccacccg cgctcgctga ccgcctgata
     5461 atgtcgcagg gtcctggtga taagtttcag ccagtcccgc tcgatcaagc gcgtctttca
     5521 cggcattgcg cctggcagtc cccttgagcg tcgacggtag cgccagcagt aaattttgcc
     5581 cgacgctgaa ctggtcaaat aacagtgcat cctggaaaag aatgccaatc tgacgctgtg
     5641 cggtgggtag catgtcaatc cgttgctcat tgaaccatag ctcacctgta caagaaaact
     5701 gtccagccag tgcaccaatc atccatgaaa acagagtgga ttttccacag ccagacggcc
     5761 ccattaacgt gacaatgtca cctttatcaa ccgtaaagtt aacgtttgtc agcaagcggc
     5821 tttctggtaa acgtagcgaa acatttttca cgcagagcat tagcggagtc cttgtctgac
     5881 ataacctacc cattttgcga ctaacgcggt cagggcaaaa ataataagcg gtaacaacag
     5941 ttgccataaa gcctgggcgg cgagaatacc gttgctgccg ccgctgctta atgccaccgc
     6001 ctcagtggtg agcgtcggaa aacgccccgc gcccagccac agcgtcggca tatactgcgc
     6061 aatactgact gaaaatccca ccgcgaaggc aatcagcaca gggcgcaaca tgagtgggca
     6121 tttcacgtaa aagaagattt tggcccgcga ccagcccagt gtttgcgcaa tcaaaattaa
     6181 ccgtgaatca atgcgctgcc aggcgggttg caggataaac agcatccacg gcattaccca
     6241 cagcagatgc ccccagacca ccgctgtcca gcttccatcc aggttcagcc atagcgccag
     6301 cgtgtactgg cccgccacca gtggcagagc gggcaataga atgggcaacc agagccacaa
     6361 ctggcgacgc tgtggccccc attccagcca cagcagtaac aggagcaaag cgatgaatgt
     6421 cgccaccagc cccattgtca ggctgttgat gagtgcttca ctattgatcg tcgactgatc
     6481 cgcgaggatc gccagcagaa ccacacacag cacaccggtt aagggtaaaa aactcgccag
     6541 cgtagtgcct ggtaataaag gtgtggcagg cttgcgaatg ccatctacgc ggggaatagt
     6601 gcgccgccag ctgcgccata gcagatagct cagcaaaacg taggcggcga gtaacagcat
     6661 cagcagcaga ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca
     6721 ctgccagcta attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc
     6781 cacgaccgaa agcgaccagg caacaatcgc cagcatcgcc attgccagcg aaggcgcgac
     6841 ggagggcaac agcagccagt tcaggcattg ccagcggctg tagcccagcg aatccagcac
     6901 aatgacctgc tgcaacagcc gtttttcgct caatactgcc gctaagatcc acagcagaaa
     6961 tgcgctttct ttcactgcaa gggtgaggcc cagaccgatg ccaaatcggt ccattggcgg
     7021 agtgaaatac gggaaatagt catatagcag ccctccgtca gcaaagagca gcagggcgct
     7081 ggtggcaaaa gccacatggg gaatggcgag cagccacggc agacgggcgc acatacgctg
     7141 ccatttcggc ccaggccata gcgccacaat caccagcagg gcaatcaaca atgccccgac
     7201 cgccgcgatg gttgtcgaca ccagcgttgc cagtaatgca tgcggtaact gtggatcggc
     7261 aaataacgcc tgccagtgtg tcaacgacaa cgcaggcgat atcagcgtga gagctgccgg
     7321 gatcagcggt gcataaatca ccgccaccat cgcccacagc aaaaagatta atgcgtaccg
     7381 taacggcgta accattcttg ttccagtgca ttcacccatc ctgcgtgcgg ttcagccagt
     7441 accggcggca gatcctgcgg cattcttgat tgcaatattt cgcgctgccc gtcaggcagt
     7501 ttttgcggat cgagaacaga aggatcgccc cagacaacgg gatctgcttt acgcagttgc
     7561 gcatcgggtg agagcaggaa attggcaact accttcgccg cagcactggc attcgcgttg
     7621 gctggaatgg tgacgaaatg cacgttgcct atcatcccct cgcgaaagcc aaaactgtaa
     7681 ctgcttgccg gcaaatcgcc gctggcgatt ttttgctgcg catgcgcggg gctaaaggtc
     7741 agcgacaggc gcaatgtgcc ggctttcagc agagcatcca tccgcgcggg cgaaggcggg
     7801 aaatcctttc cttcgcgcca caaatacgga tgtagcgcat caagatattg ccacaaggga
     7861 gcagtgacac gggcgaaagt cgcatcgtcc ggcgcttctt ttaatgcggc gggatctggc
     7921 gtcagcataa tcagcaactg ttcaagaaac gccgtgccgg taaagtccgg tgggcgcgga
     7981 taagtaaccg tgccgggatt gactttagca aactccagta aggcttgcgg cgtttgtggt
     8041 ggctgtggcg taacatcgcg gcgggcgata aacgtcagtt gtgcgccgcc ccagggcgat
     8101 tccgcacctt gtgtcggcac tgaaaaatct tcccgcaccg gcagctgtgt gtcgacatag
     8161 cgccagttgg gcagagtctc cgcccagccc gtttgtaata aattggcctc ttttaaggtg
     8221 cggaagtttt cgccgttcac ccagagcaga tccaccgagc cgcccgtttt acgtccggca
     8281 gcagcttcgg tctgaatgcg cttcaccgcg tctgcggcat ccgcgagacg aacaatcttc
     8341 aggtttatag cgtaatgggt tttcatctcg ccgctaaccc agtcgagata gcggttaatt
     8401 gcggtatcgc cgccccaggc gttaaaccag acggtttgcc ctttggcctc atttttaatt
     8461 tcctgccagt cactggcatg tgttgccaaa gaaaacagcg ataacaatcc cagcaaccac
     8521 ccacaatggc gcatcttccc tccttaagaa cgtttttccg gaatacggca cagcccctga
     8581 gcgggaatgt ccatggcaga attaaaacga gccgacaggt tttgcaggcc gatcaatgcc
     8641 gtcagttcgg tgagcgcctg agcgtcaaaa tgcgcagcca gtcgggtacg cagggcatca
     8701 tcgactgttg gcggtgtgac gcttgcggct tcggcgtact ccagcgccag ccgttcttca
     8761 tcgctaaaga gcgggctttg ccgccagtca gccactgcca gcagtttatc gttgctgccg
     8821 gtacgctcgg cgactttcat actggtgata tccacgcaaa actcacacag gcacatttgc
     8881 gcaatgcgcg cgctgacaag cgatcgcact accggatcga gcggtgagcg tttgcgctcc
     8941 agccacccaa caaacattga caccaaataa aagataaacg ggatccgccc ccaccagcga
     9001 atcgggctta gcacctcgcc gtaatggcga cgatgcagcc acgcctgcgg acgtaaaatg
     9061 aaaggaattt tgctaagcgg cggtaaaccc atcagtcatt cctcttctgg cgctcaagcc
     9121 atattttctt cgccataaaa atcaccacgg tcagggcaaa cagaataaac agtccggtga
     9181 caaaccagaa agtaccgcct gttaacatgc tgcccgccca ggaataaaca atagtcgccg
     9241 gtaattgacc aagcccggtg gcgataaaaa acgagcgaaa acgtattgaa gtcagaccgg
     9301 cagcatagct gattggatcg aaagggacaa aaggcaataa ccgacagacc aggatggtgt
     9361 gtttgccgta gcgagtgaaa aagccgtcca tactgtcaag cacggttttg ccggttaatt
     9421 tttccaccac ttcgcggccc atcactctgg cgataaaaaa gcacagcgcc gcgccggcca
     9481 tcgaactggt ccacgacagc aagccgcccc agaacgcgcc aaacagcgac gcattggcaa
     9541 aggtgatcaa aaacgcaggt agcggtgcag caatggcctg taaaatcatc aacaagaacg
     9601 agacaactgc cgccagtgcg ccgtaagact gaataaagcg ttctataccc tgttggtcca
     9661 cggcggcaaa cgccgcaacg ctgcgattga taaactcatg gacgccggga agcagcgccc
     9721 atgcgagcag catagcgaac aacaggatga tgagggttat acggtagtac cagattttac
     9781 gcgattgtat cttcatcatc ctttatcctg gctttattcg gtgattttaa aggtaaccgt
     9841 tgccagcgtg ttatccgccg ggagaactga ggcattgcct ttgaatttca cttcgccgcg
     9901 tttttccacc gcaccataag tgtatgttgc attgctgacg atgccgaccg ggcagctatc
     9961 cagacacacc aggcaacccg ttttcttctc ttctgctgcc gttaaattac cgccaaagcg
    10021 catgtccagt ttcttgccat tactgtcaac aatcacttca tcgaaggaat acgcttttgc
    10081 cgccccttgc cagttgaccg aaatatccag tttgctgcct gtgacaagag tcgtttcttt
    10141 attatccatc gtcatatttt cgcccggcgt accacctgcc tctttcaggg cttcataaaa
    10201 tgctttcggg gtcgcataag ccataaacag cgatttatgt ccgttggagc catctttaaa
    10261 cacaataccg tgacgagtgt cgtcggtgag gaaacgacca ttaatttgca ccagcatggt
    10321 taccgtatgg ttattggcat cgacgcgcag cggttggctg ttcgaaagtc cgtcgtattc
    10381 cacttttccc gccgcattct ctttttgatc gcagccgcta agcccggcaa cagccagtaa
    10441 gcagagtgca gccagtcctt ttttccatga cactgaataa tgttgcgaca tcgttctatc
    10501 cttcattttt tgggtgagta agtgggctgc ggcgcgaagc agacagatcc acatgtttgt
    10561 gtcgggcgta gagttttgcg agctggacga gaataaacag cgccaggccc gccagacaga
    10621 gttgtaaaat aaagcgcagc gtaatgcctt cattggcgag atcgcttgcc atcacggtat
    10681 aaataacaat accgggcagg gtcgtgagtg ccgaaataag ggtataaggc cagaaggcga
    10741 tggtggttaa tccgtaagcg taattttgaa tattgtaagg aaacaacggg attaagcggg
    10801 tcagaataag aaaatcaata ccgttacgcg caatgccttt ttcaatggcc tggaaggtat
    10861 ggctatggcc cacgtatttc agcagtaaat cacgtcccag ccagcgcgcc agcaggaatg
    10921 agctcgagga ggccagcgtt gcggcaatta atgagagtag tgtccctaag agcgggccaa
    10981 aaactattcc tccagcgatc accagtatgc ttcctggtaa cagaaagagg gtggcaatga
    11041 tgaataacag aatatagagg ctataaccga acaatccgct ctggcgaatg agtgtctgta
    11101 agtggggtaa atcggtgagc agatcgaata aaccgaaagc gtggatagcg taaatgacca
    11161 gcgcaaaaat aagacaggca aacagaaagt tacgctcagc gttcatcgtg gagattccta
    11221 tatcaaactt gataatagag aagtggtcag acaactaaaa acgcagtgac ggtataacaa
    11281 aggacggcag gcaacaaatc attactgaca gtttgttgat ttttctcaag gttaattctc
    11341 ctgacccagt ttgagccagg agagctgcta aattagcggc ggaaggtcgc ccagacgggg
    11401 gcgtgatcgg acggtttttc catgctgcga atttcatagt cgatgccggt ttctacgcaa
    11461 cattctgcca gcggttggct ggcgagcagc aggtcgatgc gcagaccacg gttatcgtca
    11521 aaaccttttg agcggtaatc aaaccatgag aaacgatctg ctgtttgcgg attagcatgg
    11581 cggaaggtat cgaccaaccc ccagctcatc agtctgtcca tccattcgcg ctcttccggc
    11641 aggaaagagc atttaccggt acgcagccag cgcttacggt tttcttcgcc aatgccgata
    11701 tccagatctg tagggctgat attcatatcg cccataatca gtaccggatt atcacgtttg
    11761 agttcggttt ccaggtagtt ttgcagattc tgataaaact gcgcttttgc cgggaatttt
    11821 atcggatggt cgcggctttc accctgcggg aagtaaccgt tgatcacggt gacattaccc
    11881 agcagtgagg ggatttccgc cataataatc cgccgctgcg cctcttcgtc gtcaccggga
    11941 aagccgcgac gcacggcaat cggcgtctct ttggtcagca gcgccacgcc ataatggcct
    12001 ttctgcccgt gataaaacac gttgtagccg agcttcgcca cctcttcgag cggaaacata
    12061 tcgtcatgaa cttttgtctc ctgcaggcca atcacatccg gttggtgctt ttcgacgatg
    12121 gcttcaagct ggtgaggtct ggcgcgcagg ccgttgatat taaaagagac aaatttcata
    12181 gtcgctgcca tttattcaga taaagagtgc gtggatggta gcagaatttc gcgttgctta
    12241 ccgcctgttg ttaagtgatt tgatcgatgg ttgttaatga tgtcaacgat ggcgcaaaaa
    12301 atgcccgctt ttggtgcgcg ctgcgtcaga atggcgcagt aatttccagt aaatttcgtc
    12361 aatgatcaca attccagaat tatattttac ttctgcataa cattgcgttt tttattcttt
    12421 ttattcaccc acatcgcaaa cgtattcact ttatatgcac tttaaatgca tatggtttgg
    12481 ttataactcc ttgatttcca gttagcctcc gccgtttatg cacttttatc actggctggc
    12541 acgaaccctg caatctacat ttacagcgca aacattactt attgttaaca tataaataac
    12601 gaattattta ctgtagaggt cgctatgtct cagccaatta cgcgtgaaaa ctttgatgaa
    12661 tggatgatac ctgtttacgc tccggcaccc tttataccgg tacgtggcga aggttcgcgc
    12721 ttgtgggatc agcaggggaa agagtatatc gacttcgcgg gtggcattgc ggtgaacgcg
    12781 ctgggccatg cgcatccgga actgcgtgaa gcgctgaacg aacaggcgag taagttctgg
    12841 cataccggca acggttacac caacgagccg gtactgcgac tggcgaaaaa attgatcgac
    12901 gccacgtttg ccgatcgcgt cttcttttgt aactccggtg cggaagccaa cgaagcggcg
    12961 ctaaaactgg cgcgtaaatt cgctcacgac cgctacggca gccataagag cggcatcgtg
    13021 gcgttcaaaa atgcgtttca tggtcgcacg ctgtttactg tcagtgcggg tgggcagcca
    13081 gcctattcac aggattttgc gccactgccg gcggatattc gtcatgctgc atataacgat
    13141 attaactctg ccagcgcgct gattgacgac tctacctgtg cggtgattgt cgaacccatc
    13201 cagggggaag gcggtgtggt gccagccagc aacgcgtttt tacaaggtct gcgtgaattg
    13261 tgtaaccgcc acaatgcgct gttgattttt gatgaagtac aaaccggcgt cgggcgcacc
    13321 ggggaactgt atgcctatat gcactacggc gtgacgcctg atctgttaac taccgccaaa
    13381 gcgctgggcg gcggtttccc ggtcggtgcg ttgttggcaa ccgaagagtg cgcccgcgtg
    13441 atgaccgttg gcactcatgg caccacctat ggcggtaatc cgctggcctc ggcggtggca
    13501 ggcaaagtgc tggagctcat caacacacca gagatgctta atggcgttaa acagcgtcac
    13561 gactggtttg ttgagcgtct taatactatt aatcaccgct atggtttgtt cagtgaagtt
    13621 cgcggcttag gtttgctgat tggctgtgta ctgaatgccg attacgccgg gcaagcgaaa
    13681 cagatctctc aggaagcggc gaaagcaggc gtgatggtac tgattgcggg tggcaacgtg
    13741 gtgcgttttg cgcctgcgct caatgtcagc gaagaagagg tgacgaccgg actggatcgc
    13801 tttgcagctg cttgcgaaca ctttgttagc cgaggttcat catgatggtc atccgtcccg
    13861 ttgagcgatc agatgtctcg gcgctgatgc agcttgccag caaaacgggc ggcggcctga
    13921 cgtcgcttcc cgccaatgaa gccacgcttt cggcgcgtat cgaaagggca atcaaaacct
    13981 ggcaaggcga actgcccaaa agtgagcagg gctatgtgtt cgtgctggaa gatagcgaga
    14041 caggcaccgt ggcggggatt tgtgccattg aggtggcggt tgggctgaac gatccctggt
    14101 acaactatcg cgtcggcacg ttggttcacg cctcaaaaga gctgaatgtc tataacgcat
    14161 tgccgacgct gtttctcagt aacgatcaca ccggcagcag cgagctgtgc acgctgtttc
    14221 tcgacccgga ctggcgcaaa gagggcaacg gctatttgct gtcgaaatcg cgctttatgt
    14281 ttatggcggc ttttcgcgac aagtttaatg acaaagtggt tgctgaaatg cgcggggtga
    14341 ttgacgaaca cggctattca ccgttctggc aaagcctcgg taaacgcttc ttttcgatgg
    14401 attttagccg cgccgatttt ctctgcggca ccgggcaaaa ggcatttatt gcagaactga
    14461 tgccgaaaca tccgatctat acccactttt tatcccagga agcccaggac gtcatcggtc
    14521 aggtacatcc gcaaaccgcg cctgcccgcg cggtgctgga gaaagaaggt tttcgttacc
    14581 gcaactatat cgacatcttc gacggcgggc caacgcttga gtgtgacatc gaccgcgtgc
    14641 gcgccattcg taaaagtcgg ctggtggaag tggcagaagg gcagcctgcg cagggcgatt
    14701 ttccggcctg cctggtcgcc aatgaaaatt atcaccattt ccgcgtggtg ctggcgcgta
    14761 ccgatccggc aaccgagcgt ttgattttaa ccgccgcaca actggatgcc ctcaaatgcc
    14821 acgcagggga tcgcgttcgt ctggtgcgcc tgtgcgcaga ggagaaaaca gcatgacttt
    14881 atggattaac ggtgactgga taacgggcca gggcgcatcg cgtgtgaagc gtaatccggt
    14941 atcgggcgag gtgttatggc aaggcaatga tgccgatgcc gctcaggtcg aacaggcttg
    15001 tcgggcagcc cgtgcggcgt ttccgcgctg ggcgcggctc tcattggctg aacgtcaggt
    15061 cgttgtcgaa cgctttgccg gattgctgga aaggaataaa ggcgaattaa ccgcgattat
    15121 tgccagagaa acgggtaagc cgcgctggga agcggcaacc gaagtgacgg cgatgatcaa
    15181 taaaatcgcg atatcaatta aggcgtatca cgttcgtacc ggcgagcagc gtagtgaaat
    15241 gccggacggt gcggcgagcc tgcgacattg cccgcacggc gtgctggcgg tgtttgggcc
    15301 gtataatttc cctggtcatt tgccgaacgg acatatcgtt ccggcattgc tggcaggtaa
    15361 caccattatc tttaaaccca gcgaactgac accgtggagt ggcgaagcgg taatgcgttt
    15421 atggcagcag gctggcttgc cgccgggcgt actgaacctg gtgcagggcg ggcgtgaaac
    15481 gggtcaggcg ctgagtgcgc tggaggatct cgacggtttg ctgtttaccg gtagcgccaa
    15541 taccggctac cagctgcatc gccagctctc cggtcagccg gagaaaattc tcgcccttga
    15601 gatgggcggt aataatccgc taattatcga tgaggtggcg gatatcgacg cggctgtcca
    15661 tctgaccatt cagtcggcgt ttgtcacagc cgggcaacgc tgcacctgcg cccgccgttt
    15721 attgctcaaa agcggagcgc agggcgatgc gtttcttgct cgtctggttg ccgtcagcca
    15781 gcgattaacg ccgggcaact gggatgacga accgcagccg tttattggcg ggctgatttc
    15841 tgaacaggcc gcacagcagg tggttactgc ctggcagcaa ctggaagcga tgggcggacg
    15901 aaccctgctt gcgccgcgct tattacaatc agagacatcg ttgctgacgc cggggatcat
    15961 tgaaatgaca ggcgttgctg gcgtaccaga tgaagaggtg ttcggaccgt tattgcgcgt
    16021 ctggcgttat gattctttcg aggaagcgat tctaatggcg aataacactc gcttcggact
    16081 ctcttgcggt ctggtttccc ccgagcggga aaaattcgat caactgttgc tggaggcgcg
    16141 ggcggggatt gttaactgga acaaaccgct tactggtgct gccagtaccg cgccattcgg
    16201 cggcattggt gcatccggta accatcgccc cagcgcctgg tatgccgcag attactgcgc
    16261 atggcctatg gcgagcctgg agtcggactc gttaactttg cccgcaacgc ttaaccccgg
    16321 gctggatttt tccgatgagg tggtgcgatg aacgcctggg aagttaattt cgacgggctg
    16381 gtagggctga cgcatcatta cgctggcctg tcgtttggta atgaagcctc tacccgtcac
    16441 cgttttcagg tgtctaaccc gcgacaggcg gcaaaacagg gcttactgaa aatgaaagcc
    16501 cttgccgatg cgggattccc ccaggccgtg atcccgccgc acgagcgtcc gtttattccg
    16561 gtgctgcgtc agttgggatt cagtggtagc gatgagcagg tactggaaaa agttgcacgc
    16621 caggcaccgc actggctttc cagcgtcagt tccgcttcgc caatgtgggt agccaatgcg
    16681 gcaacgatcg cgccatctgc cgatacgctg gatggcaaag tgcatctcac cgttgccaac
    16741 ctgaacaata aatttcaccg ctcgctggaa gcgcacgtca ccgaatcact tttaaaagcg
    16801 atttttaacg acgaagagaa atttagcgtt cattcggcgt tgccgcaggt agcgttgctc
    16861 ggtgatgagg gggcggcaaa ccacaatcgt ctcggcggtc attacggtga gccgggtatg
    16921 caactttttg tctacgggcg agaagaaggc aatgataccc ggccttcccg ttatccggcg
    16981 cgacagactc gcgaagccag cgaggcggtg gcaaggctga atcaggtgaa tccccaacag
    17041 gtgattttcg cccagcaaaa cccggacgtt atcgaccagg gcgtttttca taatgacgtg
    17101 attgccgtga gtaaccgcca ggtgctgttt tgccaccaac aggcgttcgc tcgccagtca
    17161 cagttactgg caaacctgcg tgcgcgggtg aacggtttta tggcgataga agttccggca
    17221 actcaggttt ccgtgtcgga tgcggtgtct acctatctgt ttaacagcca actgttgagc
    17281 cgcgatgatg gttccatgat gttggtgctg cctcaggagt gtcgggaaca cgccggagta
    17341 tggggttatc tcaatgaact ccttgccgct gacaacccga ttagcgaact aaaagtcttt
    17401 gatttacgtg aaagcatggc gaatggcggc ggcccggcgt gcctgcggtt gcgggtggta
    17461 ttgacagaag aagaacgccg ggcggtgaat ccggcggtga tgatgaacga tacgctgttt
    17521 aatgcgctca atgactgggt ggatcgttac taccgcgatc gccttactgc tgccgatctg
    17581 gccgacccgc aattgctgcg cggagggcgg gaagcactgg atgtattgag ccaattactg
    17641 aatctcggtt cggtttatcc gttccagcgc gagggagggg gcaatggata attttcttgc
    17701 tctgacctta acgggtaaaa aaccggttat caccgagcga gaaatcaacg gcgttcgctg
    17761 gcgctggctg ggcgatggtg tgctggaact gacgccatta acgccaccgc aaggcgcact
    17821 ggtgatttca gcgggaatac acggtaatga gacggcacct gtggagatgc tggacgcgtt
    17881 gcttggcgcg atatctcacg gcgagatccc gttacgttgg cggttgctgg tgatcctcgg
    17941 gaatcctcct gcgctgaagc aagggaaacg ttattgccat agcgatatga atcgaatgtt
    18001 tggcggtcgt tggcagctat ttgctgaaag cggagaaacc tgtcgggcgc gcgaactgga
    18061 acagtgcctg gaagattttt atgacctggg caaagaatct gtgcgctggc accttgatct
    18121 acataccgca attcgtggct ccttgcatcc gcagttcggt gtattaccgc aacgcgacat
    18181 tccctgggac gagaaatttc tgacgtggct gggtgcggcg gggctggagg cgctggtgtt
    18241 ccatcaggaa cctggtggta cgtttaccca tttcagcgcc agacattttg gcgcgctggc
    18301 ctgtacgctg gaacttggca aagcgttgcc ctttgggcaa aacgatcttc gccagtttgc
    18361 agtaactgcc agcgcaattg ctgcgctgct atctggtgag agtgtcggta tcgtgagaac
    18421 accgccgctc cgttatcggg tggtttcgca aattactcgc cactcgccgt ccttcgaaat
    18481 gcatatggca agtgacacgc tgaattttat gccgtttgag aaaggaacat tgctggcgca
    18541 ggacggagag gaacgtttta ccgtaaccca tgatgtagag tatgtgttat tccctaatcc
    18601 gttggtagcg ttgggattac gcgcgggatt aatgctcgaa aaaataagct aatacttacc
    18661 cgcagaaatc attctgcggg tattaagttg ctttcttata aattaataca g
//
LOCUS       DAPGWD010000073        18564 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPGWD010000073 DAPGWD010000000
VERSION     DAPGWD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.073"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            236..928
                     /locus_tag="RHX88_002579"
     CDS             236..928
                     /locus_tag="RHX88_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDV2231251.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
     gene            951..2378
                     /gene="mdtD"
                     /locus_tag="RHX88_002580"
     CDS             951..2378
                     /gene="mdtD"
                     /locus_tag="RHX88_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDV2231252.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2344..3336)
                     /gene="rbsR"
                     /locus_tag="RHX88_002581"
     CDS             complement(2344..3336)
                     /gene="rbsR"
                     /locus_tag="RHX88_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDV2231253.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3340..4269)
                     /gene="rbsK"
                     /locus_tag="RHX88_002582"
     CDS             complement(3340..4269)
                     /gene="rbsK"
                     /locus_tag="RHX88_002582"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV2231254.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4395..5285)
                     /gene="rbsB"
                     /locus_tag="RHX88_002583"
     CDS             complement(4395..5285)
                     /gene="rbsB"
                     /locus_tag="RHX88_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDV2231255.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5310..6275)
                     /gene="rbsC"
                     /locus_tag="RHX88_002584"
     CDS             complement(5310..6275)
                     /gene="rbsC"
                     /locus_tag="RHX88_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDV2231256.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6280..7785)
                     /gene="rbsA"
                     /locus_tag="RHX88_002585"
     CDS             complement(6280..7785)
                     /gene="rbsA"
                     /locus_tag="RHX88_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDV2231257.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7793..8212)
                     /gene="rbsD"
                     /locus_tag="RHX88_002586"
     CDS             complement(7793..8212)
                     /gene="rbsD"
                     /locus_tag="RHX88_002586"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDV2231258.1"
                     /translation="MKKGTVLNSDISSMISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8379..10247)
                     /gene="kup"
                     /locus_tag="RHX88_002587"
     CDS             complement(8379..10247)
                     /gene="kup"
                     /locus_tag="RHX88_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDV2231259.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10470..11966
                     /gene="ravA"
                     /locus_tag="RHX88_002588"
     CDS             10470..11966
                     /gene="ravA"
                     /locus_tag="RHX88_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDV2231260.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11960..13411
                     /gene="viaA"
                     /locus_tag="RHX88_002589"
     CDS             11960..13411
                     /gene="viaA"
                     /locus_tag="RHX88_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDV2231261.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13416..14408)
                     /gene="asnA"
                     /locus_tag="RHX88_002590"
     CDS             complement(13416..14408)
                     /gene="asnA"
                     /locus_tag="RHX88_002590"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDV2231262.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14560..15018
                     /gene="asnC"
                     /locus_tag="RHX88_002591"
     CDS             14560..15018
                     /gene="asnC"
                     /locus_tag="RHX88_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDV2231263.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15108..15551
                     /gene="mioC"
                     /locus_tag="RHX88_002592"
     CDS             15108..15551
                     /gene="mioC"
                     /locus_tag="RHX88_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDV2231264.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15930..17819
                     /gene="mnmG"
                     /locus_tag="RHX88_002593"
     CDS             15930..17819
                     /gene="mnmG"
                     /locus_tag="RHX88_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDV2231265.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17883..18506
                     /gene="rsmG"
                     /locus_tag="RHX88_002594"
     CDS             17883..18506
                     /gene="rsmG"
                     /locus_tag="RHX88_002594"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDV2231266.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVCPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4656 a         4839 c         4600 g         4469 t
ORIGIN      
        1 agaaccccgc tgacgcggcg gcgtgtttgc cgttgttccg tgtcagtggt ggcgcattat
       61 agggagttat tccggcctga caagaggaaa tttaaaataa ttttctgacc gcgcattttt
      121 tcagcaaaag ccccgttaat gtgccataaa acaagcaatt tgccgttttt gcaaccgtaa
      181 tcacacttcc tggtggcata ctaaggagga ataaaaagaa aaaggaagat tagtaatgcc
      241 tttaagcgca caacagttag ctgcacagaa aaacctttcg tatgttctgg ctgagaagct
      301 ggcgcaacgc atcttaaaag gtgaatatga acccggcacc attttgcctg gtgagattga
      361 gctgggcgag caatttggag tgagtcgtac agcggtacgc gaagcggtca aaacgttaac
      421 ggcaaaaggg atggttttac cgcgaccgcg aattggtacc cgggtcatgc cacaatcaaa
      481 ctggaatttt cttgatcagg aattgcttac ctggtggatg acagaagaga actttcatca
      541 ggtcatcgat cactttctgg ttatgcgtat ctgtctggag ccgcaagctt gcctactggc
      601 tgcaacggtt ggcaccgcgg aacagaaggc gcatctcaat acgttaatgg ccgaaatggc
      661 ggcattaaaa gagaactttc gccgtgaacg ctggatcgaa gtcgatatgg cctggcatga
      721 gcatatctat gaaatgagcg ccaatccgtt tttgacctca ttcgcctcgc tattccattc
      781 ggtttatcac acttacttca cgtcaattac cagcgacaca gtgataaagc tggatctgca
      841 ccaggcgatt gtcgatgcga ttgtccaaag cgatggcgac gcggcattta aagcttgtca
      901 ggcgctgcta cgctcacctg ataagtgaca acccgataac aggacattga atgagcgata
      961 aaaagaagcg cagtatggcg ggtttgccgt ggatcgcggc gatggccttc ttcatgcagg
     1021 cacttgatgc cactattctg aataccgcct tacccgcaat cgctcatagc cttaatcgtt
     1081 ctcctctcgc gatgcaatca gccatcatca gttatacgct gacggtggcg atgcttattc
     1141 cggtaagcgg atggctagcc gatcgcttcg gtacgcgtcg catttttacc cttgccgtga
     1201 gtctgttcac attgggttct ctggcctgcg cactttctaa ttcgctacca cagctggttg
     1261 tcttccgggt tattcagggg ataggcggcg caatgatgat gcctgttgct cggctggcct
     1321 tactgcgcgc ttatcctcgt aatgaacttc ttccagtatt gaattttgtc gccatgccgg
     1381 gtctggtggg gccaatttta ggccccgttc ttggcggcgt gctggtcacc tgggcaacct
     1441 ggcactggat atttttaatc aatatcccca taggtattgc gggccttctt tacgcgcgca
     1501 aacatatgcc caatttcacc accgcacgac gcagattcga tatcactggc tttttgctgt
     1561 ttggcctcag ccttgttctc ttctcaagcg gaatagagct attcggggaa aagattgtcg
     1621 ccagctggat tgccttgacg gtaattgtca ccagcatcgg gttactgctt ctctatattc
     1681 tccatgcgcg acacacgcca aacccattaa tttcattaga tttatttaaa acccgcactt
     1741 tctcaatcgg tatcgtaggc aatattgcaa cccgtctggg gaccggttgt gtaccgttcc
     1801 ttatgccatt gatgttacag gtaggatttg gttatcaggc gtttattgcc ggctgtatga
     1861 tggcaccgac agcgttaggt tccattattg caaaatcgat ggttacccaa gtcttacgtc
     1921 gtctgggcta tcgccatacg ttagtgggga tcacggtgat tattgggcta atgatcgctc
     1981 agttctcttt gcaatcaccg gcaatggcga tatggatgct gatcttgccg ttgtttatat
     2041 tagggatggc tatgtcgacg caatttaccg cgatgaatac catcacactt gccgatctga
     2101 ccgatgacaa cgccagcagc ggtaacagtg ttctggcggt cacgcagcaa ctgtcgatta
     2161 gtttaggcgt tgctgtaagt gcggccgtcc ttcgcgttta tgaagggatg gaaggcacaa
     2221 cgactgtcga acaattccac tatacgttta tcacgatggg cattattact gttgcttcag
     2281 cagcaatgtt catgcttctg aaaacaaccg atggtaataa tttgatcaaa agacagcgta
     2341 aatctaagcc gaaccgcgtt ccatcagaat cggagtaagt tgtaatcgtt gttgctgaag
     2401 ggtcggctgg gttatccgat ggatgagtac atcaatcgcc agctccccca gttcatcttt
     2461 cggttggtgg atagtggtta atggtggcgt cataaagctt gccagttcga tatcgtcata
     2521 gccaatcacc gcgatatcct gcggaacctg taactctgcc tgatataacg cctggtaaac
     2581 gccaacagcc atagcgtcat ttccggtaaa gacggcctga ggacgcagcg gatgtgataa
     2641 cagttggcgc atggcgtcaa acccgccgtt aaattcaaaa tcaccagtga cttcatagcc
     2701 atcaggaatg ttgagacccg cacgtttcat cgccgcccga taaccttcca accgcaggcg
     2761 cgccggagtt ttatccagcg gaccggtaat acaggcgata cgggtatgac ctttatcgat
     2821 cagatattgc gttgctaagt ctccgcccag caacgagtta tcctgaataa gatcgctgtc
     2881 gccatcgaac ggagcccagt ccatcatcac agtaggcact gtcggataac gttgcatgat
     2941 ttcacgcgaa ggttgatgcg tttcggtgca cagtaacagc aagccatcaa cgcgtttttg
     3001 catcagcgtt tccagattgc gattcatccg ctgttcatcg ccttcggtat tgcaaaggac
     3061 gagactataa ccgcgttcga agcagctgcg ttcaacgcca cgcaccagtt ctgaatagaa
     3121 aggattggta ctggcagtga tcaacatgcc aatggtatgt gtttgattga gtttgaggct
     3181 acgcgccagg gctgatggcg cgtaattgag ttctttaatc gccgcttcaa ctttggcggt
     3241 aatcgcttca ctgacgaagc gatctttatt gataacgtga gaaactgttg aggtagaaac
     3301 gcccgccagg cgggcaacat ctttcattgt agccaagcgt cacctctgcc tgtctaaaaa
     3361 tgcgtcgatc tcttcacgcc acggtacgga aggttgtgcg cctttacgtg ttacggcaat
     3421 cgcagcggca gcatgggcaa accgaatcgc ctctggcaat ggtttttctt ccagcaacgc
     3481 cgtgattaac gcgccgttaa aggtatctcc ggcagcaatg gtatcgacag cctgcacccg
     3541 gaatccagga acgcgctgac cttcaccatt cacgctagcc catacaccac gacttcctaa
     3601 agtaatcagt acagtacgga tacctttttc atgcagtacc tgcgccgcct tcgctgcatc
     3661 ttcatcattt tcaacacgaa taccggtgag cttttctgct tccgtttcgt ttggcgtaat
     3721 aatgtccacc agcgccagca gttcgtcagg aagttcgcga gccggagccg ggttaagcgc
     3781 aacgatagtc ttattttgat gggcgatttt cgccgctgcc atcacacttt cgagtggtga
     3841 ttccagctgc attaataatg ctgacgcgtt ggcaatacgc tcacgttgcg cttccaccag
     3901 cgccggggaa agggcagcat tagcgccggc atgaataccg atgacattct caccttcgcc
     3961 attaacaaaa atcagcgcca cacctgttga ttcgcctttg atcacgctga ccggagtaat
     4021 atcaatgtta tcagtggcga gctgctggcg aacgctctca ccaatgctgt catcacccgt
     4081 acaggcaata aacgcgatat tcgcaccgct acgcccagca gccacagcct gattcgcgcc
     4141 tttgccgcca aatgcaacct gatagtggtt accggttacg gtttcgcctg gagtaggaaa
     4201 agattgaaga ttaagaatgt ggtcagcatt aatgctgcca agaacaacga ggctgcctgc
     4261 gttttgcata ttcgggatgt ccataaaatg cgccaccgtg ttagggtggc gcgtgccctg
     4321 cttttcttta tattacacgt tcatctgtcg atgacgtatt tatgtcacca tcaggtcata
     4381 caacctgatt aaaactactg cttaacaacc agtttcagat caaccggata cttagcctga
     4441 actttctcgc ctttcagcac tttatctgcg gtttcgacgc ctttcgcgcc aatctgatcg
     4501 ggtagctgag cgatagtcgc tgctagtttg ccatcattca ccgctttttc gccatccggt
     4561 gtaccgtcaa atccgacgac catcacatcc gatttaccgg cagtttgcag tgcgcgcagc
     4621 gcgcccagcg ccatttcatc attctgcgcg aatacagcct gaacatccgg atgagcggtc
     4681 aacaggttct gcattacgtt caaaccttta gtgcgatcaa aatctgctgg ctggctggca
     4741 agaacattaa acttgtgagc agcaacggcc tgctggaagc cttcgccacg ttcacgggct
     4801 gcggatgtac cagcaatgcc ttgcagctcg ataactttgg caccttcacc cgctttcttc
     4861 gcgatgtaat caccggcgat tttgccaccc agtacgttat cagaagcaat gtggctcacc
     4921 acttcacctt tcgttgcctg gcggtcaaga gtgataaccg ggatgttcgc ctggttagcc
     4981 atcttcacag cattacctac tgcgtcggag tcggtcgggt taatcagcag aattttggtg
     5041 ccgcgaacgg ttaagtcctg cacgttcgcc agctctttcg ccgggttgtt ctgggagtcc
     5101 agcaccacca ggttatagcc aagcttatcc gcctctttct gcgcgccatc tttcagcgat
     5161 acaaagaacg ggttgttaag cgtggagacc accagcgcga tggtgtcttt tgccatcgca
     5221 ttcgcactga cggtggcgct tagcgcaaca gcggaaacca gggtagccag ttttttcatg
     5281 ttcatattca agatgtcctg tagtcgttat tactgctttt tgttgtctac cagcaccgcc
     5341 agcaaaatca ccaccgcttt gacgatcatc tggtaatagg aggaaacacc taacaaattc
     5401 aatccattat taaggaagcc aagaattaat gcgccgatca acgtcccaac aatgcgacct
     5461 tttccgcccg ccagactcgt accgcccaga accaccgcag caatagcatc cagctcatag
     5521 ccagtccccg ccgtgggttg tgcggaggag agacgcgcca cttcaatgat cccggccagc
     5581 gatgccagca gaccacaaag agaatagacg atgattttga ttttattgac gttgatacca
     5641 gaaagacgcg ttgccgcttc gttgccgccc agcgcgtaga tgtaacgccc cagacgcgtg
     5701 tgatgcagca tgtaccaggc cgcgaggaag acaatcccca tgatccagac tggcgtcggt
     5761 acgcccagcg gacgaccaat accaaaccag ccaaacagat cggcgttctc agtaaagccg
     5821 gtattcactg ggctaccgtt ggtataaacc atggtcacgc cgcgcagtaa aagcatcata
     5881 accagcgtag cgataaacgc ctgaacgcga cctttcgcta caatcacccc ggttaccgca
     5941 ccaattgcgg cacctaacgc gagagcggca gcgacagcca ccagcgcatt gacttcaatg
     6001 ccgacgatag atgcagcaac tgcgccggtt agcgccaaca gagaacctac agacaagtcg
     6061 atgcccgacg tcaggatcac cagcgtcatc ccgaccgcca taatggcgtt caccgaggtt
     6121 tgctggagaa tattgaataa gttattgatg gtgaaaaagt tcgggcttaa cgtcgagaca
     6181 atcgcgatca gcaccagcag agcgataagc gatttctgct ccatcagcca cgctttcgtg
     6241 aaataacggc gaccagagac agtctgggtt gtcatttttt tactcctgat tcacgcgatt
     6301 aagcttgccc acagcggcag ccattaacac ttcctgggtg gcctgctcac gagtaaattc
     6361 cccgctgaga tgcccttcat gcatgacgat gatgcgatcg ctcatgccta atacttctgg
     6421 catctccgat gacaccagaa tgatgctcaa gccatcggct ttgaactggt taatcagttg
     6481 atagatctct tttttcgcgc cgacatctac gccacgggta ggttcatcaa ggatcaacac
     6541 tttggggcgt gtcatcagac cgcgggcaat cgccactttt tgctgattgc caccggaaag
     6601 cagaccaatt gcctgttcca tcgacggtgt tttcacatta aacagacgaa tgaaatcact
     6661 cacagcctgc tgttcatcgg catgcttcaa actgccgcca gcgcggctga agtagcgcag
     6721 cgctgtcagc gacatgttct cttttactga catgcccaac actaaaccgt cacgtttacg
     6781 gtcttcggag atatacacaa tgccgcttgc caggccatcc tgcggtgaac gggtaacgac
     6841 ttcatgccca tccagggtga cgtaaccgct ggtgcgcggt agtgcgccgt agagcacttt
     6901 catcagttcg gtacgacctg cgcccatcaa accagagacg ccaagaattt cgcctttgcg
     6961 taaagtaaaa gagacatcgt taacgccagg tccgcagaga ttatcgactt tcaggcggat
     7021 atctcccggc gctttgtcca ggtgtggata ttgatcttct agcttgcggc ccaccatcat
     7081 ctcaatcagc gaatcttcgg tcagtgatgc cacttcgcgc tcagcaataa attgcccgtc
     7141 acgaaaaacg gtaacgtcat cgcaaatctc gaagatttct ttcatgcggt gggagatata
     7201 gacaataccg cggccttgcg atttcagctc gcgtatgacg cggaacaggg attcggtttc
     7261 ggtatcggtc agcgcatcgg tcggttcatc cataatgatg actttcgact caaagctcag
     7321 cactttggcg atttcaacca tttgctggtc accgatggaa agatcgccca ccagcttgtc
     7381 gcttttaaag cgcaggttaa gtttagccag caatttatcc gcttcggcat acatggtttt
     7441 ccagtcaatt ttgccaaagc gattaacaaa ctcacgaccg aggaaaatgt tttcggcaat
     7501 ggtcaactgc gggatcaggt tcagttcctg atggataatc ccaatcccgg cttcctggga
     7561 agatttcggc ccggtaaatg tcgtttcttt ccccagccat aaaagcgtgc cggcatcgcg
     7621 agcatagata ccagtaagca ctttcatcat ggtggattta cccgcgccgt tttcgcccac
     7681 cagcgccatc acgcggcccg gatagacatt taacgctgcg cccgagaggg cttttacgcc
     7741 cgggaaggct ttatcgatgc ctttaagctg aagtaatgct tccatgacgg cctcagaacg
     7801 tcacgccagc acagagaatg atattcgcat acggagaaca ttctccgctg cgaattaccg
     7861 cctgactttc tgcggtttgt tgtttgaatt gttcatgcgt ggtgtaacga atttcaatgg
     7921 tatttccctg gtgtttttgc agctgctcaa ggtgagtgag caacgtttcg tggagttgcg
     7981 gattatgttg tttgatctct tccgcgataa tggcagcctc gacctgcatt tcatttgtga
     8041 cgacgcccag cacctgcata aaagaaggta caccctgggt taatgccata tcgatacgcg
     8101 ttgtactttt ggggatgggt aaaccagcat cacacaccac cagcgtatcg gtatgtccca
     8161 gacgggagat catcgatgaa atatcagaat taagaacggt gccttttttc atttttttct
     8221 ccatcagcga aacgtttcgc tgacccgcag tttaatcctg actgagttgg gaaaaccatc
     8281 gtgacgttac ggaaatgtga tcaacctcga aacgttttac atggtgatta accatgaaaa
     8341 caaaaacgcc cccttatgga gaagggaggc gtctggcgtt agatttcgac ctgagtaccc
     8401 agttcgataa ccctgtttgg cggaatttca aattgatctg gtgcacgcag cgcattacgt
     8461 tgcagcagca agtacagctt gccgcgcaga cgcaaatacc acgggcgttt gccgaggatc
     8521 aacgactcat gcgacataaa gaaggaggtt tccatcatcc ggcaacttaa tccttccaga
     8581 ccgcagcggt ggaaaacttc ttccacgttt ggcgtttctc gccaaccata acttgccacc
     8641 acgcgccaga aagtgggcga cagttgttca atctgtaccc gacggacatt atggacatat
     8701 ggcgcgtctt cggtacgcag agttaacaga atcacccgct catgcaatac cttgttatgt
     8761 ttaaggttat gcatcagcgc aaagggaatg acgttgattg cacgcgacat atacaccgcg
     8821 gtcccgggca cgcgaacagg cggtgatttc tccagcgaag caatcatcgc ttccagagag
     8881 ttaccatgtt catgcatccg ccgcagcaag cggaaacgct cgctcttcca ggtggtcatc
     8941 acgataaaca tcacagtacc gaggctcaat ggcaaccagc cgccggagag cagtttatcg
     9001 aggttagcgg tgaacaatgg aatatcgaca caaaggaaag caatcaggat cagcgcaaca
     9061 aaatacttat tccagtgcca gttctgacgt gccacggtag tcgagagaat agacgtcagc
     9121 accatggttc cggtcaccgc aatcccgtac gccgccgcca ggttgctgga gtgctcaaag
     9181 ctgacaatca caatcacgac cgcgacatag agcatccagt tcacaaacgg aatatagatt
     9241 tgccctgact ccatttcgga ggtgtgaata atgcgcatcg gcgacaaata tcccagacgt
     9301 accgcctgac gcgtcaatga gaagacgcca gagataaccg cctgcgaggc aattaccgtc
     9361 gccagtgcgg cgatgatcag cagcgggatc agcgcccagt ccggtgccaa caggaagaac
     9421 gggttcttaa tcgcttccgg gttctttaac aacagcgctc cctgaccgaa gtaattaagg
     9481 gtcaaggaag gtaatacgac ggtgaaccac gccaggcgaa tagggaactt accaaagtgc
     9541 cccatatcag catacagcgc ctcgaccccc gtaatcgaca acaccactgc ccctaatgca
     9601 ataaaagaaa ccgttttgta ttcaaggaag aaatgcaccg cccacattgg attcagcgca
     9661 tgcagcactt ccgggttagc aataatgcta cgtaacccca gtcctgccag aatcaaaaac
     9721 caggtcagca tgatcggcgc aaacagctta ccgaccatag ccgtgccatg tttttgaatc
     9781 ataaataata aagtgagaac gataattgag aggggaacta tccaggtatc cagctgcggg
     9841 gcgacgattt ccagaccttc aatggctgac atcaccgaaa tagcgggtgt tatgacgact
     9901 tcaccataga aaaagctgcc gccgattagc cccataatca ccagcattga tgtggttcgc
     9961 gccgacgtat tacgcccggc aagcgacatc aacgtcagga tccccccctc accggcgtta
    10021 tctgcccgca tcacgaaggt gagatattta atggaaacca caaagattag cagccagaag
    10081 atcagcgata aaaagccaaa cacggcatcg cgttcaacgc caaaaccaaa ctggccggac
    10141 aaacattcac gaagtgtata taacgggctg gtaccaatat cgccgtagac aactccaatc
    10201 gccgcgaggg taatcgcggg caatgattgc ttattatcag tgctcataga ctagtctttc
    10261 gttgaaatat gaaatgtgtg cttagtccct tggcccacaa aaaagcgcac agtatgcacg
    10321 attaacggca aaatcgtact cctaaatgcg gccacattaa cctggctcaa agaaaaatac
    10381 cccccctttg agactatttt agccaccagc aagaaacgtc tatactcgca atttacgcag
    10441 aacttttgac gaaaggacgc cacttcatta tggctcaccc tcatttatta gcggaaagaa
    10501 tttcccgcct gagcagttcg ttggaaaagg ggctttatga acgtagccac gccatccgct
    10561 tgtgtttatt agcggcatta agtggtgaaa gtgtgttcct ccttggcccg ccaggtattg
    10621 ccaaaagttt gatcgcccgg cgcttaaaat tcgcctttca gaatgcccgc gcgtttgaat
    10681 atctgatgac ccgcttctcc acgccggaag aagtttttgg tcccctttct attcaggcgc
    10741 taaaagatga agggcgctat gaacgtttaa ccagcggtta cctgccggaa gcagaaattg
    10801 tctttctgga tgagatctgg aaagcgggcc cggcaattct taataccttg ctcaccgcca
    10861 ttaacgagcg ccagttccgc aacggtgcac acgtagaaaa aatcccgatg cgtctgctgg
    10921 tggcggcctc caacgagctg ccggaagcag acagcagtct ggaagcgtta tatgaccgca
    10981 tgctgattcg tctgtggtta gataaagtgc aggataaagc gaatttccgc tccatgctga
    11041 ccagtcaaca ggatgaaaac gacaatccgg ttcctgatgc cttgcaggtc acagatgaag
    11101 aatatgaacg ctggcagaaa gagattggtg aaattacgct gcccgatcat gtatttgagc
    11161 tgatttttat gctgcgccag caactggata aattaccgga tgcgccttat gtctcggatc
    11221 gtcgctggaa aaaagcgatc cgcttattgc aggccagcgc cttttttagc ggtcgcagtg
    11281 ctgttgcccc ggtagatctc attttgctga aagattgcct gtggtatgac gcgcaaagcc
    11341 tgaatttgat acaacaacaa attgatgtat tgatgaccgg tcacgcctgg caacagcaag
    11401 ggatgttgac tcgccttggc gcgattgtgc aacgtcacct gcaactccag cagcaacaaa
    11461 gcgataaaac agccttaacg gtaattcgtc tgggcggcat tttcagccgt cgtcagcagt
    11521 atcaacttcc tgttaacgtt actgcttcca ctctgactct gctgctgcaa aaaccgttaa
    11581 aattgcatga tatggaagtg gttcatatct cctttgagcg taacgcgctg gaacagtggc
    11641 tgagtaaagg tggtgaaatt cgcggcaaac tgaacggtat cggctttgcc cagaaattga
    11701 atctggaagt ggatagcgcc caacatcttg ttgtacgcga tgtaagttta caaggcagta
    11761 cgctggcact tcccggttca tcggctgaag gtctgccagg tgaaataaaa caacaactgg
    11821 aagagcttga aagtgactgg cgcaagcaac acgctttatt cagcgaacag caaaaatgtc
    11881 tgtttatccc tggcgactgg ttaggtcgca ttgaagccag cctacaggat gtcggcgcac
    11941 agattcgcca ggcacaacaa tgctaacgct ggatacgctt aatgtgatgc tggccgtcag
    12001 cgaagaggga ttgatcgaag agatgatcat cgcgctgctg gcctcaccgc agctggcagt
    12061 cttctttgaa aaattcccac gactgaaggc ggcaatcact gatgatgttc cccgctggcg
    12121 tgaggcgctg cgcagtcggc tgaaagatgc ccgagtcccg ccggaactca ccgaagaggt
    12181 gatgtgctat cagcaaagcc agctcctctc cacgccgcag tttattgtgc agctaccaca
    12241 gatcctggac ttactgcatc gtctgaattc cccatgggca gaacaagccc gacagttggt
    12301 tgatgctaac agcacgatca cttcagcgtt acatacgctt ttcctccagc gttggcgttt
    12361 aagtctgatc gtgcaagcaa cgacgttaaa tcaacagcta ttagaagaag aacgcgaaca
    12421 actgttaagt gaagttcagg aacgcatgac gctgagcgga caacttgaac cgattctcgc
    12481 agataacaat accgcagctg gtcgtctgtg ggatatgagc gccggtcagc ttaaacgtgg
    12541 cgactatcag ttgattgtga aatacggtga atttcttaac gaacagccgg aactgaaacg
    12601 cctggcagag cagctggggc gttctcggga agccaaatca ataccgcgca acgatgcgca
    12661 gatggaaacc ttccgcacca tggtgcgcga accggcgacg gttcctgagc aggttgatgg
    12721 tctgcaacaa agcgatgata ttttacgtct cctgccgcca gaactggcga cactagggat
    12781 aacggaactg gagtatgagt tttaccgtcg gctggtggaa aaacagttgc tcacctatcg
    12841 cctgcacggt gagtcgtggc gtgaaaaagt gatcgaacgt ccggtggtac ataaagatta
    12901 cgatgaacag ccgcgcgggc cgtttattgt ctgtgtggat acttccggct caatgggcgg
    12961 ctttaatgaa cagtgtgcga aagcgttctg cctggccttg atgcgcattg ctctcgcaga
    13021 aaaccggcgc tgctatatta tgctattttc caccgagatc gtccgttatg agctttcagg
    13081 cccacaaggc atcgaacaag caatccgttt tttaagccag cagtttcgtg gcggcaccga
    13141 tcttgccagt tgttttcgcg ccattatgga acgcttgcaa agcagggaat ggtttgatgc
    13201 cgatgcggtg gtgatttctg attttatcgc tcagcggttg cctgacgacg tgacgagtaa
    13261 agtgaaagag ctgcagcggg tacatcagca tcgctttcat gccgtggcga tgtcggcaca
    13321 cggcaaaccc ggcatcatgc gcattttcga tcatatctgg cgctttgata ccgggatgcg
    13381 aagccgcctg ctcagacgct ggcggcgata aattattaca gcagagaagg gacgctctcg
    13441 cgaacagcag ctggccatac tccacactga acctggccga tatgcggcag ttgcagcagc
    13501 agcatggtca aacgagactg gccgataccg ccgccgatgg tctgcggcat ttcaccgcgc
    13561 agcagcgcct gatgccattc cagctgcaga cgatcttcat caccggtcag cgccaactga
    13621 tgcttcaacg tgtcggcatc cacgcggatc cccatggaag aaagctcaaa cgcatcttcc
    13681 agtacagggt tccataccag aatatcgccg ttcagacccg catggcccag ctctgacggg
    13741 gtgctccagt catcataatc cggtgcgcgc acgtcgtgac gatgaccatc actcagcttg
    13801 ccgccaatcc cgacgaggaa taccgcgcca agatctttcg caatcgcccg ctcacgccct
    13861 ttggcatcga gatccggata acgagacagt aactcctggc tgtgtacgaa gtggatctga
    13921 tccggcagga acggtgccaa accaaactct tcgctaactg cagcttcggt tgctttaatt
    13981 cccgcccaga tcgcctctac cgtgcttttc agagtcgaga attgacgctc accgtcgccc
    14041 attacgcgtt cccagtccca ctggtcaaca tagaccgagt gcaacggaga aagacgctct
    14101 tcatcggggc gaagggcttt catgtgcgtg tacagccctt cgcccgcgct gaagtcgtgt
    14161 tgccctaagg tctgacgttt ccacttcgcc agtgaatgaa ccacttcgaa ctgggcatca
    14221 ggcagagctt tcacttttac ctgcaccgct ttttcacagc ccgacaagtt atcctgcgtg
    14281 ccatccccca cacggctaag aatcggcgcc tggacttcga tcagccccag acgttcttcc
    14341 agttggcgag aaaagtgaga tttcacgaag ctaatttggc gttgtttggc aatgtaagcg
    14401 gttttcattt tttatactcc tgcgtcctgt tgcttatgat taagcaacaa aaaccgacac
    14461 acatgcaata atcattcaat aaaaagccta acacactttt gattcattaa aaaagaaggc
    14521 taaaatagaa tgaatcatca atctgcataa gaaaatccta tggaaaatta tctgatcgac
    14581 aatctggacc gtggcatcct ggaagcatta atgggcaatg cgcgcaccgc ttacgccgaa
    14641 ctggcgaaac aatttggcgt cagtccgggg acgattcacg ttcgagtaga gaaaatgaag
    14701 caggcgggga tcattaccgg ggcgcgtatt gatgtcagcc cgaagcagct cggttatgac
    14761 gtaggctgct ttatcggcat tatattaaag agcgccaaag actacccttc cgcgctggca
    14821 aagctggaaa gccttgatga agtcactgaa gcctattaca caaccggcca ctacagcatc
    14881 tttataaaag taatgtgccg ttcgatcgac gctctccagc atgtacttat caacaagatc
    14941 caaacaattg atgaaattca gtccaccgag acattgatcg tcctgcaaaa cccgatcatg
    15001 cgtactatca agccctgatc ggctttttta atcccatact tttccacagg tagatcccaa
    15061 cgcgttcaca gcgtacaata cgccactctt aataaaggtg gcggtttatg gcagatatca
    15121 ctcttatcag cggcagcacc ctcggcggtg ccgaatatgt agcagaacac ctggctgaaa
    15181 agctggaaga ggcgggtttt accaccgaaa cgctgcacgg tccgctgttg gaagatttat
    15241 ctgcctcggg gatctggctg attatcagct ccacccacgg tgccggagat attccggaca
    15301 acctttctcc tttctatgaa gcattgcagg aacagaagcc cgatctttct gcggtccgct
    15361 ttggcgcaat cggtattggt agtcgtgaat atgacacctt ttgtggggct atcgataaac
    15421 tcgaggcaga actcaaaaat tccggtgcaa aacagacagg cgaaacactg aagatcaaca
    15481 ttcttgatca cgacattccg gaagatccgg cagaagaatg gctgggatcg tggattaatt
    15541 tactcaaata agtatacaga tcgtgcgatc tactgtggat aactctgtca ggaagcttgg
    15601 atcaaccggt agttatccaa ggaacaacgg ttgttcagtt tttgagttgt gtataacccc
    15661 tcattctgat cccagcttat acggtccagg atcaccgatc attcacagtt aatgatcctt
    15721 tccaggttgt tgatcttaaa agccggatcc ttgttatcca cagggcagtg cgatcctaat
    15781 aagagatcac aatagaacag atctctaaat aaatagatct tctttttaat acccaggatc
    15841 ccaggtcttt ctcaagccga caaagttgag tagaatccac ggcccgggct tcaatccatt
    15901 ttcataccgc gttatgcgag gcaatcacca tgttttatcc ggatcctttt gacgtcatca
    15961 tcattggcgg gggtcatgca ggcaccgagg ccgcgatggc cgcggcgcgt atgggtcaac
    16021 agactctgct tttgacacac aatatcgaca ctctggggca gatgagctgc aacccggcga
    16081 tcggcggtat tgggaaggga catctggtaa aagaagtgga tgcactcggc ggtctgatgg
    16141 cgaaagcgat cgatcaagcg ggtatccagt ttaggatact aaacgcaagc aaaggaccgg
    16201 cggttcgcgc tacccgagct caggcggatc gtgtgctcta ccgtcaggcg gtacgtacgg
    16261 cgctggagaa ccaaccgaac ctgatgatct tccagcaggc ggttgaagat cttattgtcg
    16321 aaaacgatcg cgtggtcggt gctgttaccc aaatgggact gaagttccgt gccaaagccg
    16381 tcgtgctcac cgttgggacg ttcctcgacg gtaaaattca tatcggtctg gataattaca
    16441 gcggtggccg tgctggtgat ccgccgtcca ttccgctttc tcgccgtttg cgtgaactgc
    16501 cgctgcgcgt tggtcgtctg aaaaccggga caccaccgcg tattgatgct cgaactatcg
    16561 actttagtgt gctggcgcaa cagcatggcg ataacccaat gccggtattc tcgtttatgg
    16621 gcaatgcgtc ccagcatccc cagcaggtgc cgtgttatat cactcatacc aacgagaaaa
    16681 cccatgatgt gatccgcagt aacctcgatc gtagcccaat gtatgcaggg gtgatcgaag
    16741 gtgtcggccc acgctactgc ccgtcgatcg aagacaaagt catgcgcttc gccgacagaa
    16801 atcagcatca gatcttcctt gaaccggaag gactgacctc taacgaaatt tatccgaacg
    16861 gtatctccac cagcctgccg ttcgatgtgc agatgcaaat cgtccgctct atgcagggga
    16921 tggaaaacgc gaagatcgtg cgtccgggtt atgccattga gtatgacttc ttcgatcctc
    16981 gcgacctgaa accgacgctg gagagcaagt ttatccaggg gctgttcttt gctggtcaga
    17041 ttaacggcac taccggttac gaagaagccg ctgcgcaagg tttgctggcc ggtcttaacg
    17101 ctgcccgtct gtctgctgac aaagaaggtt gggctccggc acgttctcag gcgtatctcg
    17161 gcgtactagt tgatgacctg tgcactttag gaaccaaaga accgtatcgt atgtttactt
    17221 cgcgcgcaga atatcgtctg atgctacgcg aagataatgc ggatctgcgt ttgactgaaa
    17281 tcggtcgtga actgggcctg gtggatgacg aacgttgggc gcgctttaac gagaaacttg
    17341 agaatatcga gcgtgagcgt cagcgtctga aatcgacctg ggtaaccccg tcggcggaag
    17401 ctgcagccga agtgaatgct cacctgactg cgccgctttc ccgtgaagcc agtggtgaag
    17461 atctgctgcg tcgtccggaa atgacttatg aaaaattaac cacgctgacg ccgtttgccc
    17521 ctgcgttgac agacgaacag gcggcggaac aggttgagat tcaggttaaa tacgaaggtt
    17581 atatcgcgcg ccagcaagat gagatcgaaa agcagctgcg taacgagaac accctgctac
    17641 ccgcgacact ggattaccgc caggtatccg gtctttctaa cgaagtgatc gccaaactta
    17701 acgatcacaa accagcctct atcggccaag cttcgcgtat ttctggcgtc acgcctgcgg
    17761 ccatctccat tctgctggtg tggctgaaaa aacagggtat gctgcgtcgt agcgcataac
    17821 gcattaaaaa tgcctggtaa gcacccgctt accaggcaac gcatcaagaa caggtaatca
    17881 ccgtgctcaa caaactctcc ttactgctga aagacgcagg tatttcgctt accgatcacc
    17941 agaaaaacca gcttattgcc tacgtgaata tgctgcataa atggaacaaa gcgtacaacc
    18001 tgacttcggt ccgcgatcct aatgagatgc tggtacgcca tattctcgat agcattgtgg
    18061 tggcaccgta tctgcaaggt gaacggttta tcgatgtcgg caccggacca ggactgccag
    18121 gcattccact ctctatcgtg tgtcctgaag cccatttcac tctgttggat agccttggta
    18181 aacgcgtgcg tttccttcgt caggtgcaac atgagcttaa actggagaat attgaaccag
    18241 tacagagcag ggtagaagag tttccttcag agccgccatt tgatggcgta attagccgcg
    18301 cttttgcctc tctgaacgat atggtgagct ggtgccacca tcttcctggt gagcaaggcc
    18361 gtttctacgc gctgaaaggg caaatgccgg aagatgaaat cgctttgttg cccgaagaat
    18421 atcaggtcga atcagtggtt aaacttcagg ttccagccct ggatggcgaa cgtcatctgg
    18481 tggtgattaa agcaaataaa atttaatttt tatcaaaaaa atcataaaaa attgaccggt
    18541 tagactgtta acaacaacca ggtt
//
LOCUS       DAPGWD010000074        18557 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPGWD010000074 DAPGWD010000000
VERSION     DAPGWD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.074"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..527
                     /locus_tag="RHX88_002595"
     CDS             <1..527
                     /locus_tag="RHX88_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDV2231267.1"
                     /translation="LADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSAAVIKWYA
                     ERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNARDSAEVV
                     ETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDE
                     LIPGLLSEYIEQSG"
     gene            502..2427
                     /locus_tag="RHX88_002596"
     CDS             502..2427
                     /locus_tag="RHX88_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDV2231268.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2424..2630
                     /locus_tag="RHX88_002597"
     CDS             2424..2630
                     /locus_tag="RHX88_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDV2231269.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2627..4228
                     /locus_tag="RHX88_002598"
     CDS             2627..4228
                     /locus_tag="RHX88_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV2231270.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4209..5528
                     /locus_tag="RHX88_002599"
     CDS             4209..5528
                     /locus_tag="RHX88_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDV2231271.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQACVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5538..5870
                     /locus_tag="RHX88_002600"
     CDS             5538..5870
                     /locus_tag="RHX88_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDV2231272.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            5926..6951
                     /locus_tag="RHX88_002601"
     CDS             5926..6951
                     /locus_tag="RHX88_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDV2231273.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     THARGLRTYGCIQDADALSEGINASPRYPKNWKTSGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            6993..7388
                     /locus_tag="RHX88_002602"
     CDS             6993..7388
                     /locus_tag="RHX88_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDV2231274.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDGAGQDSSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            7400..7753
                     /locus_tag="RHX88_002603"
     CDS             7400..7753
                     /locus_tag="RHX88_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDV2231275.1"
                     /translation="MADFDNLFDAAIVRADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7765..8343
                     /locus_tag="RHX88_002604"
     CDS             7765..8343
                     /locus_tag="RHX88_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231276.1"
                     /translation="MAIKGLEQAIENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGKARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8340..8735
                     /locus_tag="RHX88_002605"
     CDS             8340..8735
                     /locus_tag="RHX88_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231277.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8743..9483
                     /locus_tag="RHX88_002606"
     CDS             8743..9483
                     /locus_tag="RHX88_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231278.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            9499..9921
                     /locus_tag="RHX88_002607"
     CDS             9499..9921
                     /locus_tag="RHX88_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDV2231279.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLAWLKEQEKKAESSGN
                     LQVSVEELIRGGAFLVAMSLWHNHPQKTKLPSMNEAITQIEQEVLTTWPTEAIAQAEN
                     VVLRLSGMSEFVVNNAPEQTEDAGPAEPVSAGKFSTVS"
     gene            9948..10337
                     /locus_tag="RHX88_002608"
     CDS             9948..10337
                     /locus_tag="RHX88_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDV2231280.1"
                     /translation="MGRPDWRAMLAGMSSTEYADWHRFYSTHYFHDVLLDMHFSGLTY
                     TVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAGGVRFGPDGNEVIPTS
                     PDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10330..12909
                     /locus_tag="RHX88_002609"
     CDS             10330..12909
                     /locus_tag="RHX88_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV2231281.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WVESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WTAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     NRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            12906..13235
                     /locus_tag="RHX88_002610"
     CDS             12906..13235
                     /locus_tag="RHX88_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231282.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13235..13933
                     /locus_tag="RHX88_002611"
     CDS             13235..13933
                     /locus_tag="RHX88_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV2231283.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            13938..14681
                     /locus_tag="RHX88_002612"
     CDS             13938..14681
                     /locus_tag="RHX88_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231284.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSYLPWLLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPNFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            14618..15250
                     /locus_tag="RHX88_002613"
     CDS             14618..15250
                     /locus_tag="RHX88_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV2231285.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRISGRDVSTSGLTAQLHEVLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTSILFSLGASMVLGGVAQMLAPKARTPTAASTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15310..>18557
                     /locus_tag="RHX88_002614"
     CDS             15310..>18557
                     /locus_tag="RHX88_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV2231286.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVFGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQGYPNTALV
                     GVKVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRVSDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSS"
BASE COUNT         4224 a         4718 c         5861 g         3754 t
ORIGIN      
        1 agctggctga cattttcggt gcgagtatcc gtaccattca gaactggcag gaacagggaa
       61 tgcccgttct gcgaggcggt ggcaagggta atgaggtgct ttatgactct gccgccgtca
      121 taaaatggta tgccgaaagg gatgctgaaa ttgagaacga aaagctgcgc cgggaagttg
      181 aagaactgcg gcaggccagc gagacagatc tccagccagg gactattgag tacgaacgcc
      241 atcgacttac gcgtgcgcag gccgacgcac aggagctgaa aaatgccaga gactccgctg
      301 aagtggtgga aaccgcattc tgtactttcg tgctgtcgcg gatcgcaggt gaaattgcca
      361 gtattctcga cgggatcccc ctgtcggtgc agcggcgttt tccggaactg gaaaaccgac
      421 atgttgattt cctgaaacgg gatatcatca aagccatgaa caaagcagcc gcgctggatg
      481 aactgatacc ggggttgctg agtgaatata tcgaacagtc aggttaacag gctgcggcat
      541 tttgtccgcg ccgggcttcg ctcactgttc aggccggagc cacagaccgc cgttgaatgg
      601 gcggatgcca attactatct cccaaaagaa tccgcatacc aggaagggcg ctgggaaaca
      661 ctgccctttc agcgggccat catgaatgcg atgggcagcg actacatccg tgaggtgaat
      721 gtggtgaagt ctgcccgtgt cggttattcc aaaatgctgc tgggtgttta tgcctacttt
      781 atagagcata agcagcgcaa cacccttatc tggttgccga cggatggtga tgccgagaac
      841 tttatgaaaa cccacgttga gccgaccatc cgcgatattc cgtcgctgct ggcgctggct
      901 ccgtggtatg gcaaaaagca ccgggataac acgctcacta tgaagcgttt ttccaatggt
      961 cgtggcttct ggtgcctggg cggtaaagcg gcaaaaaact accgtgaaaa gtcggtggat
     1021 gtggcgggtt atgatgaact tgctgccttt gatgaggata ttgaacagga aggctctccg
     1081 acgttccttg gcgacaaacg tattgaaggc tcggtctggc caaagtccat ccgtggctcc
     1141 acgcccaaag tgagaggcac ctgccagatt gagcgtgcag ccagtgaatc cccgcatttt
     1201 atgcgttttc atgttgcctg cccgcactgc ggggaggagc agtatcttaa atttggcgac
     1261 aaagagacgc cgtttggcct caaatggacg ccggatgacc cctccagcgt gttttatctc
     1321 tgcgagcata atgcctgcgt catccgccag caggagctgg actttactga tgcccgttat
     1381 atctgcgaaa agaccgggat ctggacccgt gatggcattc tctggttttc gtcatccggt
     1441 gaagagattg agccgccgga cagtgtgacc tttcacatct ggacggcgta cagcccgttc
     1501 accacctggg tgcagattgt caaagactgg atgaagacga aaggggatac gggaaaacgt
     1561 aaaaccttcg tgaacaccac gctcggtgag acatgggaag cgaaaattgg cgaacgtccg
     1621 gatgctgagg tgatggcgga gcggaaagag cattattcag cgcccgttcc tgaccgtgtg
     1681 gcttacctga ccgccggtat cgactcccag ctggaccgct acgaaatgcg cgtatgggga
     1741 tgggggccgg gtgaggaaag ctggctgatt gaccggcaga ttattatggg ccgccacgac
     1801 gatgaacaga cgctgctgcg tgtggatgag gccatcaata aaacctatac ccgccggaat
     1861 ggtgcagaaa tgtcggtatc ccgtatctgc tgggatactg gcgggattga cccgaccatt
     1921 gtgtatgaac gctcgaaaaa gcatgggctg ttccgggtga tccccattaa aggggcatcc
     1981 gtctacggaa agccagtggc cagcatgcca cgtaagcgaa acaaaaacgg ggtttacctt
     2041 accgaaatcg gtacggatac cgcgaaagag cagatttata accgcttcac actgacgccg
     2101 gaaggggatg aaccgcttcc cggtgccgtt cacttcccga ataacccgga tatttttgat
     2161 ctgaccgaag cgcagcagct gactgctgaa gagcaggtcg aaaaatgggt ggatggcagg
     2221 aaaaaaatac tgtgggacag caaaaagcga cgcaatgagg cgctcgactg cttcgtttat
     2281 gcgctggcgg cgctgcgcat cagtatttcc cgctggcagc tggatctcag tgcactgctg
     2341 gcgagcctgc aggaagagga tggtgcagca accaacaaga aaacactggc agattacgcc
     2401 cgtgccttat ccggagagga tgaatgacgc gacaggaaga acttgccgct gcccgtgcgg
     2461 cactgcatga cctgatgaca ggaaaacggg tggcaacggt acagaaagac ggacggcgag
     2521 tggagtttac ggccacttcc gtgtctgacc tgaaaaaata cattgcggag ctggaagtgc
     2581 agaccggcat gacacagcga cgcaggggac ctgcaggatt ttatgtatga aaacgtccac
     2641 cattcccacc cttctggggc cggacggcat gacatcgctg cgtgaatatg ccggttatca
     2701 cggcggtggc agcggatttg gtgggcagtt gcgggcgtgg aacccaccga gtgaaagtgt
     2761 ggatgcagcc ctgctgccca actttacccg tggcaatgcc cgcgcagacg atctggtacg
     2821 caataacggc tatgccgcca acgccatcca gttgcatcag gatcatatcg tcgggtcttt
     2881 tttccgactc agtcatcgcc caagctggcg ctatctgggc atcggggagg aagaagcccg
     2941 tgccttttcc cgcgaggttg aagcggcatg gaaagagttt gccgaggatg actgctgctg
     3001 cattgacgtt gagcgaaaac gcacgtttac catgatgatt cgggaaggtg tggccatgca
     3061 cgcctttaac ggtgaactgt tcgttcaggc cacctgggat accagtccgt cgcggctttt
     3121 ccggacacag ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca ataccggcga
     3181 cagccggaac tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc tgggatatta
     3241 cgtcagcgag gacgggtatc ctggctggat gccgcagaaa tggacatgga taccccgtga
     3301 attacccggc gggcgcgcct cgttcattca cgtttttgaa cccgtggagg acgggcagac
     3361 ccgcggtgca aatgtgtttt acagcgtaat ggagcagatg aagatgctcg acacgctgca
     3421 gaacacgcag ctgcagagcg ccattgtgaa ggcgatgtat gccgccacca ttgagagtga
     3481 gctggatacg cagtcagcga tggattttat tctgggcgcg aacagtcagg agcagcggga
     3541 aaggctgacg ggctggattg gtgaaattgc cgcgtattac gccgcagcac cggtccgtct
     3601 gggaggcgca aaagtgccgc acctgatgcc gggggactca ctgaacctgc agacggctca
     3661 ggacacggat aacggctact ccgtgtttga gcagtcactg ttgcggtata tcgctgccgg
     3721 gctgggtgtc tcgtatgagc agctttcccg gaattacgcc cagatgagct actccacggc
     3781 acgggccagt gcgaacgagt cgtgggcgta ctttatgggg cggcgaaaat tcgtcgcatc
     3841 ccgtcaggcg agccagatgt ttctgtgctg gctggaagag gccatcgttc gccgcgtggt
     3901 gacgttacct tcaaaagcgc gcttcagctt tcaggaagcc cgcagtgcct gggggaactg
     3961 cgactggata ggctccggtc gtatggccat cgatggtctg aaagaagttc aggaagcggt
     4021 gatgctgata gaagccggac tgagcaccta cgagaaagag tgcgcgaaac gcggtgacga
     4081 ctatcaggaa atttttgccc agcaggtccg tgaaacgatg gagcgccgtg cagccggtct
     4141 taaaccgccc gcctgggcgg ctgcggcatt tgaatccgga ctgcgacaat caacagagga
     4201 ggagaagagt gacagcagag ctgcgtaatc tcccgcatat tgccagcatg gcttttaatg
     4261 agccgctgat gcttgaaccc gcctatgcgc gggttttctt ttgtgcgctt gcaggccagc
     4321 ttgggatcag ccgcctgacg gatgcagtat ccggcgacag cctgactgcc ggagaggcac
     4381 ccgcggcgct ggcgttatcc ggtgatgatg acggaccacg acaggcccgc agttatcagg
     4441 tcatgaacgg catcgccgtg ctgccggtgt ccggtacgct ggtcagccgg acgcgggcgc
     4501 tgcagccgta ttcgggaatg accggttaca acggcattat cgcccgtctg caacaggctg
     4561 ccagcgatcc gatggtggac ggcattctgc tggatatgga cacaccgggc gggatggtgg
     4621 cgggagcatt tgactgtgct gacatcatcg cccgtgtgcg agacataaaa ccggtatggg
     4681 cgctggccaa cgacatgaac tgcagtgcag gtcagctgct tgccagcgcc gcctcccggc
     4741 gtctggtcac gcagaccgcc cggacaggct ccatcggcgt catgatggct cacagtaatt
     4801 acggtgctgc gctggagaaa cagggcgtgg aaatcacgct gatttacagc ggcagccata
     4861 aggtggatgg caacccctac agccatctac cggatgatgt ccgggaaaca ctgcagtccc
     4921 ggatggatgc aacccgccgg atgtttgcac agaaggtgtc ggcatatacc ggcctgtccg
     4981 tgcaggctgt gctggatacc gaggctgcag tgtacagcgg tcaggaggcc attgatgccg
     5041 gactggctga tgaacttgtg aacagtaccg atgcgatcac cgtcatgcgt gatgcactgg
     5101 atgcacgtaa atcccgtctc tcaggagggc gaatgaccaa agagactcaa tcaacaactg
     5161 tttcagccac tgcttcgcag gctgacgtta ctggcgtggt gcaagcgacg gagggcgaga
     5221 acgccagcgc tgcgcagccg gacgtgaacg cgcagatcac cgcagcggtt gcggcagaaa
     5281 acagtcgcat tatggggatc ctcaactgtg aggaggctca cggacgcgaa gaacaggcat
     5341 gcgtgctggc cgaaaccccc ggtatgaccg tggaaacggc ccgccgtatt ctggccgcag
     5401 caccacagag tgcacaggcg cgcagtgaca ctgcgctgga tcgtctgatg cagggggcac
     5461 cggcaccgct ggctgcaggt aacccggcat ctgatgccgt taacgatttg ctgaacacac
     5521 cagtgtaagg gatgtttatg acgagcaaag aaacctttac ccattaccag ccgctgggca
     5581 acagtgaccc ggctcatacc gcaaccgcgc ccggcggatt gagtgcgaaa gcgcctgcaa
     5641 tgaccccgct gatgctggac acctccaccc gtaagctggt tgcgtgggat ggcaccaccg
     5701 acggtgctgc cgttggcatt cttgcagttg ctgctgacca gaccagcacc acactgacgt
     5761 tctacaagtc cggcacgttc cgttatgagg atgtgctctg gccggaggct gccagcgacg
     5821 agacgaaaaa acggaccgcg tttgccggaa cggcaatcag catcgtttaa ctttaccctt
     5881 catcactaaa ggccgcctgt gcggcttttt ttacgggatt tttttatgtc gatgtacaca
     5941 accgcccagc tgctggcggc aaatgagcag aaatttaagt ttgatccgct gtttctgcgt
     6001 ctctttttcc gtgagagcta tcccttcact acggagaaag tctatctctc acaaattccg
     6061 ggactggtaa acatggcgct gtacgtttcg ccgattgttt ccggtgaggt tatccgttcc
     6121 cgtggcggct ccacctctga atttacgccg ggatatgtca aacccaagca tgaagtgaat
     6181 ccgcagatga ccctgcgtcg cctgccggat gaagatccgc agaatctggc ggacccggct
     6241 taccgccgcc gtcgcatcat catgcagaac atgcgtgacg aagagctggc cattgctcag
     6301 gtcgaagaga tgcaggcagt ttctgccgtg ctcaagggca aatacaccat gaccggtgaa
     6361 gccttcgatc cggttgaggt ggatatgggc cgcagtgcgg cgaacaacat cacgcagtcc
     6421 ggcggcacgg agtggagcaa gcgtgacaag tccacgtatg acccgaccga cgatatcgaa
     6481 gcctacgcgc tgaacgccag cggcgtggtg aatatcatcg tgtttgaccc gaaaggctgg
     6541 gcgctgttcc gttccttcaa agccgtcagg gagaagctgg atacccgtcg cggctctcat
     6601 tccgaactgg agacagcggt aaaagacctg ggcaaagcgg tgtcttataa gggaatgtat
     6661 ggcgatgtgg ccatcgtcgt gtattccgga cagtacgtgg aaaacggcgt caaaaagaac
     6721 ttcctgccgg acaacacgat ggtgctgggt aacactcatg cacgcggtct gcgcacctat
     6781 ggctgtattc aggatgcgga tgcattgagt gagggcatta atgcgtctcc ccgttatccg
     6841 aaaaactgga agacatccgg cgatccggcg cgagagttca ccatgattca gtcagcaccg
     6901 ctgatgctgc tggctgatcc tgatgagttc gtgtccgttc aactggcgta atcatggccc
     6961 ttaggggcca ttttctctct gtggaggagt ccatgacgaa agatgaactg attgcccgtc
     7021 tccggtcgct gggtgagcaa ctgaaccgtg atgtcagcct gacggggacg aaagaagaac
     7081 tggcgctccg tgtggcagag ctggaagagg agcttgatga cacggatgac ggagccggtc
     7141 aggactcgtc tgtcagcccg gaaaatgcgc tgaccggaca tgaaaatgag gtggtatcag
     7201 cacagacgga taccgtgact gatacggctg ctctggtcac ggttgtggca ctggtgacgc
     7261 tgcataccga tgcacttcac gccacgcggg atgaacctgt ggcatttgtg ctgccgggaa
     7321 cggcgtttcg tgtctctgcc ggtgtggcag ccgaaatgac agagcgcggc ctggccagaa
     7381 tgcaataacg ggaggcgctg tggctgattt cgataacctg ttcgatgctg ccattgtccg
     7441 cgccgatgaa acgatacgcg ggtacatggg aacgtcagcc accatgacat ccggtgagca
     7501 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
     7561 gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
     7621 gctgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accgggtttc
     7681 gccggatgat ggtggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
     7741 ccgtcgccgc tgaaaggggg atgtatggcc ataaaaggtc ttgagcaggc cattgaaaac
     7801 ctcagccgta tcagcaaaac ggcggtgcct ggtgccgccg caatggccat taaccgcgtt
     7861 gcttcatccg cgatatcgca gtcggcgtca caggttgccc gtgagacaaa ggtacgccgg
     7921 aaactggtaa aggaaagggc caggctgaaa agggccacgg tcaaaaatcc gcaggccaga
     7981 atcaaagtta accgggggga tttgcccgta atcaagctgg gtaaggcgcg ggttgtcctt
     8041 tcgcgccgca ggcgtcgtaa aaaggggcag cgttcatccc tgaaaggtgg cggcagcgtg
     8101 cttgtggtgg gtaaccgtcg tattcccggc gcgtttattc agcaactgaa aaatggccgg
     8161 tggcatgtca tgcagcgtgt ggccgggaaa aaccgttacc ccattgatgt ggtgaaaatc
     8221 ccgatggcgg tgccgctgac cacggcgttt aaacaaaata ttgagcggat acggcgtgaa
     8281 cgtcttccga aagagctggg ctatgcgctg cagcatcaac tgaggatggt aataaagcga
     8341 tgaaacatac tgaactccgt gcagccgtac tggatgcact ggagaagcat gacaccgggg
     8401 cgacgctttt tgatggtcgc cccgctgttt ttgatgagga agattttccg gcaattgccg
     8461 tttatctcac cggcgctgaa tacacgggcg aagagctgga cagcgatacc tggcaggcgg
     8521 agctgcatat tgaagttttc ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga
     8581 tggagtcccg gatttatccg gtgatgagcg atatcccggc actgtcagat ttgatcacca
     8641 gtatggtggc cagcggctat gactaccggc gcgacgatga tgcgggcctg tggagttcag
     8701 ccgatctgac ttatgtcatt acctatgaaa tgtgaggacg ctatgcctgt accaaatcct
     8761 gtaatgccgg tgaaaggtgc cgggaccacc ctgtgggttt ataaggggag cggtgaccct
     8821 tatgcgaatc cgctttcaga cgttgactgg tcgcgtctgg caaaagttaa agacctgacg
     8881 cccggcgaac tgaccgctga gtcctatgac gacagctatc tcgatgatga agatgcagac
     8941 tggactgcga ccgggcaggg gcagaaatct gccggagata ccagcttcac gctggcgtgg
     9001 atgcccggag agcaggggca gcaggcgctg ctggcgtggt ttaatgaagg tgatacccgc
     9061 gcctataaaa tccgcttccc gaacggcacg gtcgatgtgt tccgtggctg ggtcagcagt
     9121 atcggtaagg cggtgacggc gaaggaagtg attacccgca cggtgaaggt caccaatgtg
     9181 ggacgtccgt cgatggcaga agatcgcagc acggtgacgg cgacaaccgg catgactgtg
     9241 acgcctgcca gcacctcggt ggtgaaaggg cagagcacca cgctgaccgt ggcattccag
     9301 ccggaaggcg caaccgacaa gagctttcgt gcggtgtctg cggataaaac aaaagccacc
     9361 gtgtcggtca gtggtatgac catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt
     9421 ccggtcgtat ccggtaatgg tgaacttgct gcggttgcag aaatcaccgt caccgccagt
     9481 taatccgggg agtcagagat gttcctgaaa accgaatcat ttgaacataa cggtgtgacc
     9541 gtcacgcttt ctgaactgtc agccctgcag cgtattgagc atcttgcctg gttgaaagag
     9601 caggaaaaaa aggctgaatc cagtggcaac ctgcaggtgt ctgtagagga gcttatcaga
     9661 ggcggggcgt ttctggtggc gatgtctctg tggcataacc atccacagaa gacaaagctg
     9721 ccgtccatga atgaagccat tacgcagatt gagcaggaag tgcttaccac ctggcccacg
     9781 gaggcaattg ctcaggctga aaacgtggtg ttacgtctgt ccggaatgtc tgagtttgtg
     9841 gtgaataatg cccctgaaca gacagaggac gccgggcctg cagagcctgt ttctgcggga
     9901 aagttttcga cggtgagctg agttttgccc tgaaactggc gcgtgagatg gggcgacccg
     9961 actggcgtgc catgcttgcc gggatgtcat ccacggagta tgccgactgg caccgctttt
    10021 acagtaccca ttattttcat gatgttctgc tggatatgca cttttccggg ctgacgtaca
    10081 ccgtgctcag cctgtttttc agcgatccgg atatgcatcc gctggatttc agtttgctga
    10141 accggcgtga ggctgacgaa gagcctgaag atgatgtgct gatgcagaaa gcggcagggc
    10201 ttgccggagg tgtccgcttt ggcccggacg ggaatgaagt tatccctact tccccggatg
    10261 tggcggacat gacggaggat gacgtaatgc tgatgacagt atcagaaggg atcgcaggag
    10321 gagtccggta tggctgaacc ggtaggcgat ctggtcgttg atttgagtct ggatgcggcc
    10381 agatttgacg agcagatggc cagagtcagg cgtcattttt ccggtacgga aactgatgcg
    10441 aaaaaaacag cggcagtcgt tgaacagtcg ctgagccggc aggcgctggc tgcacagaaa
    10501 gcggggattt ccgtcggaca gtataaagcc gccatgcgta tgcttcctgc acagttcacc
    10561 gacgtggcca cgcagcttgc aggcgggcaa agtccgtggc tgatcctgct gcaacagggg
    10621 gggcaggtta aggactcctt cggcgggatg atccccatgt tcagggggct tgccggtgcg
    10681 atcaccctgc cgatggtggg ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat
    10741 gcctggtatc agggcaactc aaccctgtcc gatttcaaca aaacgctggt cctttccggc
    10801 aatcaggcgg gactgacggc agatcgtatg ctggtcctgt ccagagccgg gcaggcggca
    10861 gggctgacgt ttaaccagac cagcgagtca ctgacggcgc tggtgaatgc cggtgtgcgt
    10921 ggtggtgagc agtttgaggc gatcagccag agtgtggcgc gtttctcctc tgcatccggc
    10981 gtggaggtgg acaaggtcgc tgaagccttc gggaagctga ccacagaccc gacgtcggga
    11041 ctgacagcga tggcacgtca gttccataac gtgacggcgg agcagattgc gtatgttgct
    11101 cagttgcagc gttccggaga tgaagccggg gcattgcagg cggcgaacga ggccgcaacg
    11161 aaagggtttg atgaccagac ccgccgcctg aaagagaaca tgggcacgct ggagacctgg
    11221 gcagacagga cagcacgggc attcaaatcc atgtgggatt cggtgctgga tattggtcgc
    11281 ccggacactg cccagggaat gctggagaaa gcagaaaagg cttttgatga ggcggacaaa
    11341 aaatggcagt ggtatcagag ccggagccac cggcgcggta aaacctcagc atttcttgcc
    11401 aatctccggg gagcatggga ggacagagcg aatgcgcaac ttgggctttc agccgccacg
    11461 ttgcaggccg atcttgaaaa ggccagagag atggcagcaa aggactgggt cgagtctgag
    11521 gcatcacggc tgaaatatac cgaagaggcg cagaaggctt acgaacgcct gcagacgccg
    11581 ctggagaaat ataccgcccg tcaggaagaa ctgaataagg cactgaaaga cgggaaaatc
    11641 ctgcaggcag attacaacac gctgatggcg gcagcgaaaa aggattatga agcgacgctg
    11701 aaaaagccga agcagtccgg cgtgaaagtg tctgcaggcg atcgccagga agacagtgct
    11761 catgctgccc tgctgacgct tcaggcagaa ctccggacgc tggagaagca tgccggagcg
    11821 aatgagaaaa tcagccagca gcgccgggat ttgtggaagg cggagagtca gttcgctgta
    11881 ctggaggagg cggcacaacg tcgccagctg tccgcacagg agaaatccct gctggcgcat
    11941 aaagatgaga cgctggagta caaacgccag ctggctgcac ttggcgacaa ggttacgtat
    12001 caggagcgcc tgaacgcgct ggcgcagcag gcggataaat tcgcacagca gcaacgggca
    12061 aaacgggccg ccattgatgc gaaaaaccgg gggctgactg accggcaggc agcgcgggaa
    12121 gccacagaac agcgtctgaa ggaacagtat ggcgataatc cgctggcgct gaataacgtc
    12181 atgtcagagc agaaaaagac ctggacggct gaagaccagc ttcgcgggag ctggatggca
    12241 ggcctgaagt ccggctggag tgagtgggaa gagagcgcca cggacagtat gtcgcaggtt
    12301 aaaagtgcag ccacgcagac ctttgatggt attgcgcaga atatggcggc gatgctgacc
    12361 ggcagtgagc agaactggcg cagcttcacc cgttccgtgc tgtccatgat gacagaaatt
    12421 ctgcttaagc aggcaatggt ggggattgtc gggagtatcg gtagcgccat tggcggggct
    12481 gttggtggcg gcgcatccgc atcaggcggt acagccattc aggccgctgc ggcgaaattc
    12541 cattttgcga ccggaggatt tacgggaacc ggcggcaaat atgagccagc ggggattgtt
    12601 caccgtggtg agtttgtctt cacgaaggag gcaaccagcc ggattggcgt ggggaatctt
    12661 taccggctga tgcgcggcta tgccaccggc ggttatgtcg gtacaccggg cagcatggca
    12721 gacaaccggt cgcaggcgtc cgggacgttt gagcagaata accatgtggt gattaacaac
    12781 gacggcacga acgggcagat aggtccggct gctctgaagg cggtgtatga catggcccgc
    12841 aagggtgccc gtgatgaaat tcagacacag atgcgtgatg gtggcctgtt ctccggaggt
    12901 ggacgatgaa gaccttccgc tggaaagtga aacccggtat ggatgtggct tcggtccctt
    12961 ctgtaagaaa ggtgcgcttt ggtgatggct attctcagcg agcgcctgcc gggctgaatg
    13021 ccaacctgaa aacgtacagc gtgacgcttt ctgtcccccg tgaggaggcc acggtactgg
    13081 agtcgtttct ggaagagcac gggggctgga aatcctttct gtggacgccg ccttatgagt
    13141 ggcggcagat aaaggtgacc tgcgcaaaat ggtcgtcgcg ggtcagtatg ttgcgtgttg
    13201 agttcagcgc agagtttgaa caggtggtga actgatgcag gatatccggc aggaaacact
    13261 gaatgaatgc acccgtgcgg agcagtcggc cagcgtggtg ctctgggaaa tcgacctgac
    13321 agaggtcggt ggagaacgtt attttttctg taatgagcag aacgaaaaag gtgagccggt
    13381 cacctggcag gggcgacagt atcagccgta tcccattcag gggagcggtt ttgaactgaa
    13441 tggcaaaggc accagtacgc gccccacgct gacggtttct aacctgtacg gtatggtcac
    13501 cgggatggcg gaagatatgc agagtctggt cggcggaacg gtggtccggc gtaaggttta
    13561 cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac agttacgccg atccggagca
    13621 ggaggtgatc agccgctggc gcattgagca gtgcagcgaa ctgagcgcgg tgagtgcctc
    13681 ctttgtactg tccacgccga cggaaacgga tggcgctgtt tttccgggac gtatcatgct
    13741 ggccaacacc tgcacctgga cctatcgcgg tgacgagtgc ggttatcacg gtccggctgt
    13801 cgcggatgaa tatgaccagc caacgtccga tatcacgaag gataaatgca gcaaatgcct
    13861 gagcggttgt aagttccgca ataacgtcgg caactttggc ggcttccttt ccattaacaa
    13921 actttcgcag taatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    13981 gtgcgccagc ggagtcgtgc ggcttcgtgg tgagaacgcc ggagggggaa agatattttc
    14041 cctgcgtgaa tatttccggt gagccggagg attatttccg gatggctccg gaggactggc
    14101 tgcaggcaga aatgcagggt gagattgtgg cgctggtcca cagccacccc ggtggtctgc
    14161 cctggctgag tgaggctgac cggcggctgc aggtgcagag ttatttgccg tggttgctgg
    14221 tctgccgggg ggcgattcat aagttccgct gtgtgccgca tctcaccggg cggcgctttg
    14281 agcacggggt gacggactgt tacacgctgt tccgggatgc ttatcatctg gcggggattg
    14341 agatgccgaa ttttcatcgc ggggatgact ggtggcgtca cggccagaat ctctatctgg
    14401 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc atcagcacaa ccgggcgatg
    14461 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    14521 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    14581 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcgcgcatct gcctttacgg
    14641 ggatttacaa cgatttggcc gccgcatcga ccttcgtgtg aaaacggggg ctgaagccat
    14701 ccgggcgctg gccacacagc tcccggcgtt tcgtcagaaa ctgagcgacg gctggtatca
    14761 ggtacggatt tccgggcggg acgtcagcac gtccggattg acggcgcagt tacatgaggt
    14821 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggggg
    14881 cgtattccag attgttctgg gggctgccgc cattgccgga tcattcttta ctgccggagc
    14941 cacccttgca gcatgggggg cagccattgg ggccggtggt atgaccagta tcctgttttc
    15001 tctcggtgcc agtatggtac ttggtggtgt ggcgcagatg ctagcaccga aagccaggac
    15061 gcccacggca gcaagtacag ataacggcaa acagaacacc tatttctcct cactggataa
    15121 catggttgcc cagggcaatg ttctgcccgt tctgtacggt gaaatgcgcg tggggtcgcg
    15181 ggtggtctct caggagatca gcacggcaga cgaaggggat ggtggtcagg ttgtggtgat
    15241 tggtcgctga tgaaaaacgt ttatgtgaaa ccgcctccgg gcggttttgt cgtttatgga
    15301 gcgtgaggaa tgggtaaagg cagcagtaag gggcatactc cgcgcgaagc gaaggacaac
    15361 ctgaagtcca cgcagttgct gagtgtgatc gatgccatca gcgaagggcc ggttgaaggt
    15421 ccggtggatg gattaaaaag cgtgctgctg aacagtacgc cagtgctgga cagtgaggga
    15481 aataccaata tctccggcgt cacggtggtg ttccgggccg gtgagcagga gcagacaccg
    15541 ccggagggat ttgaatcctc cggctccgag acggtgttcg gtacagaagt gaaatatgac
    15601 acgccgatca cccgcgccat cacgtcggca aacatcgacc gactgcgctt taccttcggt
    15661 gtgcaggcac tggtggaaac cacctcaaag ggggaccgga atccgtcgga agttcgcctg
    15721 ctggttcaga tacagcgtaa tggtggctgg gtgacggaaa aagacatcac cattaagggc
    15781 aaaaccacct cgcagtatct ggcctcggtg gtggtggata acctgccgcc gcgcccgttt
    15841 aatatccgga tgcgcagaat gacgccggac agcaccacag accagctgca gaacaaaacg
    15901 ctctggtcgt catacaccga aatcatcgat gtgaaacagg gctacccgaa cacggcactg
    15961 gtcggcgtaa aggtggattc ggagcagttc ggcagccagc aggtgagccg taattatcat
    16021 cttcgcgggc gtattctgca ggtgccgtcg aactataacc cgcagacgcg gcaatacagc
    16081 ggtatctggg acggaacgtt aaaaccggca tacagcaaca acccggcctg gtgtctgtgg
    16141 gatatgctga cccacccgcg ctacggcatg gggaaacgtc ttggtgcggc ggatgtggat
    16201 aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt cggtgccgga cggctttggc
    16261 ggcacggagc cgcgcattac ctgtaatgcg tacctgacca cacagcgtaa ggcgtgggat
    16321 gtgctcagtg atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg gcagacgctg
    16381 acgttcgtgc aggaccgacc atcagataag gtgtggacct ataaccgcag taatgtggtg
    16441 atgccggatg atggtgcgcc gttccgctac agcttcagcg ccctgaagga ccgccataat
    16501 gccgttgagg tgaactggat tgacccgaat aacggctggg agacggcgac agagcttgtt
    16561 gaagatacgc aggccattgc ccgttacggt cgtaatgtca cgaagatgga tgcctttggt
    16621 tgtaccagtc gggggcaggc acaccgagcc gggctgtggc tgattaaaac ggaactgctg
    16681 gaaacgcaga ccgtggattt cagcgtgggc gcagaagggc ttcgccatgt gccgggcgat
    16741 gttattgaaa tctgcgatga tgactatgcc ggtatcagca tcggtggtcg cgtgctggcg
    16801 gtgaacagcc agacccggac gctgacgctc gaccgtgaaa tcacgctgcc atcctccggc
    16861 accacgctga taagcctggt tgacgggcag ggtaatccgg tcagcgtgga ggtccagtcc
    16921 gtcaccgacg gcgtgaaggt gaaagtgagc cgtgttcctg acggcgttgc tgaatacagc
    16981 gtatgggggc tgaagttgcc gacgttgcgc cagcgcctgt tccgctgcgt gagtatccgt
    17041 gagaacgacg acggcacgta tgccatcacc gccgtgcagc atgtacccga aaaagaagcc
    17101 atcgtggata acggggcgca ctttgacggc gaccagagcg gcacggtgaa tggcgtcacg
    17161 ccgcccgcgg tgcagcacct gactgccgaa gtcaccgcag acagcgggga atatcaggtg
    17221 ctggcgcgct gggacacgcc gaaggtggtg aagggcgtga gctttatgct tcgcctgacc
    17281 gtggcagcgg atgacggcag tgagcggctg gtcagcacgg cccggacgac ggaaaccaca
    17341 taccgcttca cgcaactggc gctggggaac tacaggctga cagtccgggc ggcaaatgcc
    17401 tgggggcagc agggcgatcc ggcgtcggta tcgttccgga ttgccgcacc ggcagcaccg
    17461 tcgcggattg agctgacgcc gggctatttt cagataaccg ccacgccgca tcttgccgtt
    17521 tatgatccga cggtacagtt tgagttctgg ttctcggaaa agcggattac cgatatcagg
    17581 caggttgaaa ccacagcccg ctaccttggc acggggctgt actggatagc cgccagtatc
    17641 aatatcaaac cgggccatga ttattacttt tatatccgca gtgtgaacac cgttggcaaa
    17701 tcggcattcg tggaggccgt cggtcgggtg agcgatgatg cggaaggtta cctggatttt
    17761 ttcaaaggca agataaccga atcccatctc ggtaaagagc tgctggaaaa agttgatctg
    17821 acggaggata acgccagcag actggatgag ttttcgaaag agtggaagga cgctaacgat
    17881 aaatggaatg ccatgtgggg cgtcaaaatt gagcagacca aagacggcaa acattatgtc
    17941 gcgggtattg gtctcagcat ggaggacacg gaggaaggca agctgagcca gtttctggtt
    18001 gccgctaacc gtatcgcgtt tattgacccg gcaaacggga atgaaacgcc gatgtttgtg
    18061 gcgcagggca accagatatt catgaacgac gtgttcctga agcgcctgac agcccccacc
    18121 attaccagcg gtggcagccc tccggtattt tccctgacat cagacggaaa gctgaccgct
    18181 aaaaatgcgg atatcagtgg cagtgtgaat gcgaactcag ggacgctcaa caacgtcacg
    18241 attaatgaga actgtcagat taaggggaaa ctgtcagcca accagattga aggtgatatt
    18301 gtcaaaacgg tcagcaagtc tttcccccgc acgaacagtt atgccagcgg caccatcacg
    18361 gtaagaatca gtgatgatca gaagtttgac cggcaggtca tgataccgcc agtgttattc
    18421 cgcggtggta agcatgagaa tttcaacagt aataaccaac agtcatactg gtattcaacc
    18481 tgccggttaa gagtgacccg caatggtcag gagattttta atcagtccac gacggatgct
    18541 cagggcgtat tttcctc
//
LOCUS       DAPGWD010000075        18551 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPGWD010000075 DAPGWD010000000
VERSION     DAPGWD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.075"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            136..1356
                     /locus_tag="RHX88_002615"
     CDS             136..1356
                     /locus_tag="RHX88_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV2231287.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQFQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1329..1958
                     /locus_tag="RHX88_002616"
     CDS             1329..1958
                     /locus_tag="RHX88_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDV2231288.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1959..3119)
                     /gene="ybdL"
                     /locus_tag="RHX88_002617"
     CDS             complement(1959..3119)
                     /gene="ybdL"
                     /locus_tag="RHX88_002617"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDV2231289.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3228..4316
                     /gene="hcxA"
                     /locus_tag="RHX88_002618"
     CDS             3228..4316
                     /gene="hcxA"
                     /locus_tag="RHX88_002618"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDV2231290.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4326..4523)
                     /locus_tag="RHX88_002619"
     CDS             complement(4326..4523)
                     /locus_tag="RHX88_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV2231291.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4705..6810)
                     /gene="cstA"
                     /locus_tag="RHX88_002620"
     CDS             complement(4705..6810)
                     /gene="cstA"
                     /locus_tag="RHX88_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDV2231292.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6991..7404)
                     /gene="entH"
                     /locus_tag="RHX88_002621"
     CDS             complement(6991..7404)
                     /gene="entH"
                     /locus_tag="RHX88_002621"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDV2231293.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7407..8153)
                     /gene="entA"
                     /locus_tag="RHX88_002622"
     CDS             complement(7407..8153)
                     /gene="entA"
                     /locus_tag="RHX88_002622"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDV2231294.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8153..9010)
                     /gene="entB"
                     /locus_tag="RHX88_002623"
     CDS             complement(8153..9010)
                     /gene="entB"
                     /locus_tag="RHX88_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDV2231295.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9024..10634)
                     /gene="entE"
                     /locus_tag="RHX88_002624"
     CDS             complement(9024..10634)
                     /gene="entE"
                     /locus_tag="RHX88_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDV2231296.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10644..11819)
                     /gene="entC"
                     /locus_tag="RHX88_002625"
     CDS             complement(10644..11819)
                     /gene="entC"
                     /locus_tag="RHX88_002625"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDV2231297.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12194..13150
                     /gene="fepB"
                     /locus_tag="RHX88_002626"
     CDS             12194..13150
                     /gene="fepB"
                     /locus_tag="RHX88_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV2231298.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13154..14404)
                     /gene="entS"
                     /locus_tag="RHX88_002627"
     CDS             complement(13154..14404)
                     /gene="entS"
                     /locus_tag="RHX88_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDV2231299.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14515..15519
                     /gene="fepD"
                     /locus_tag="RHX88_002628"
     CDS             14515..15519
                     /gene="fepD"
                     /locus_tag="RHX88_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDV2231300.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15516..16508
                     /gene="fepG"
                     /locus_tag="RHX88_002629"
     CDS             15516..16508
                     /gene="fepG"
                     /locus_tag="RHX88_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDV2231301.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16505..17320
                     /gene="fepC"
                     /locus_tag="RHX88_002630"
     CDS             16505..17320
                     /gene="fepC"
                     /locus_tag="RHX88_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV2231302.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17317..18450)
                     /gene="fepE"
                     /locus_tag="RHX88_002631"
     CDS             complement(17317..18450)
                     /gene="fepE"
                     /locus_tag="RHX88_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDV2231303.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4356 a         5254 c         4793 g         4148 t
ORIGIN      
        1 agacagctac tataactttc atctatttat tcacagcgcg aatgaaaaat aaaacactct
       61 gctttgcagt gtttacttta taattaacag acttatctca ctgatcaccc tgtaacgttc
      121 agagagcgtc ttcagatgtc tatttataaa attcctcttc cgctcaatat tctggaagct
      181 gcacgagagc gtattacctg gacactcaac accctacccc gcgtttgtgt ctcattttct
      241 ggcggcaaag actctggttt aatgctgcat ctgacagccg aacttgcccg acaaatgggc
      301 aaaaaaatct gcgttttgtt tatcgactgg gaggcgcaat tctcttgcac cattaactat
      361 gttcagtccc tgcgcgagtt gtacaccgat gtcatcgaag agttttactg ggttgcgctc
      421 ccgcttacga cgcaaaattc cctttcacaa ttccaacccg aatggcagtg ctgggaacct
      481 gatgtcgaat gggtgcgtca gcccccgcaa gatgcgataa ccgatcctga ctttttctgc
      541 ttttaccagc ctggcatgac cttcgaacaa tttgtacgtg agtttgccga atggttttca
      601 caaaaacgtc cggcggcgat gatgatcggc atccgtgcgg atgagtccta caaccgtttt
      661 gtcgccatcg ccagtttaaa taaacaacgt tttgccgacg ataaaccatg gactacggcc
      721 gcaccaggcg gtcatagctg gtacatttac cccatttacg actggaaagt ggctgatatc
      781 tggacctggt atgcaaatca tcaaagtctc tgcaacccac tgtataacct aatgtatcag
      841 gcaggcgttc ctctgcgtca tatgcgaatt tgcgaacctt ttggcccgga gcaacgacaa
      901 ggattatggc tctatcacgt tatcgaaccg gatcgctggg cagctatgtg cgcacgagtc
      961 agcggggtaa aaagtggcgg catttacgcc ggacatgaca atcatttcta tgggcatcgg
     1021 aaaatcctca agccagaaca tttagactgg caagaatatg cattattgct gctcaatagc
     1081 atgccggaaa aaacagctga gcattaccgc aataaaatcg ccatttattt gcactggtat
     1141 cagaaaaaag gcatcgaggt tccacaaacc cagcaagggg acattggcgc gaaagatatc
     1201 ccctcctggc ggcggatatg caaagtttta ctcaataacg attactggtg tcgagcatta
     1261 tcatttagtc ctacgaaagc gaagaactat cagcgttata acgaacggat aaaaggaaaa
     1321 cgtcaggaat gggggatact atgcaacaac gattaacgca ggatttaacg caatttctcg
     1381 ccagcttgcc ggaagatgat cgcatcaaag cgattaatga aatccgcatg gcgatccatc
     1441 aggttagccc ctttcgtgaa gagccagtgg attgcgttct gtgggttaaa aacagccaac
     1501 ttatgcctaa tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgaaaa
     1561 aatccattga aatagatggt tttacccaac ccattgtggt cacgcatacg gataaaaatg
     1621 ccctggaaat cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa
     1681 aattacgctt aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca
     1741 tcgccgcgac tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg
     1801 aaatcgtccg tgaacttagc cagttgggat gggacgataa taaaattggc aaagagctgg
     1861 gaatggacag cgacgaagtg ctgcgcctga agcaaattaa cggcctgcaa gagttgtttg
     1921 ccgaccgtca atattcccgc gcctggacgg ttaaatagct aaagctggcg caggcgttca
     1981 gctgctgcca gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg
     2041 aagggatcgg cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc
     2101 cactggcaaa actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag
     2161 tatgtacctt cacacggtaa aatctccagc cggctttcat ttaaagcatt caccagaata
     2221 tcgcgcttct ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc
     2281 atatcagcaa gcgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc
     2341 ttgcgaattt cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccggtc
     2401 atatgatagg tcttgccaaa tgaagaaacc gccactgccc gctcacgcag ctgcggatgc
     2461 gccagcacac tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta
     2521 atgacaaaaa tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc
     2581 cagatagttg cactggggtt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt
     2641 aataatgcgg caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgc
     2701 ttcactattc ccccagaaag cgcgatggcg ggggagtaac tgtcatagct gggatcaaaa
     2761 caaatcactt catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc
     2821 gcccctgccg ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc
     2881 gttttctgag caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt
     2941 gccccctggg caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca
     3001 ggaaagcctt gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg
     3061 aaaatagtgg tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt
     3121 tttattgtac ctgacgaagt tgcgtgttga aggcactata acacgatgct agtatttggc
     3181 aatcaagacg tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc
     3241 ctatccgcgt ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc
     3301 tgcacgattt tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg
     3361 ccattgctgc ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata
     3421 ttttgtttcg tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg
     3481 acgatcgcag cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc
     3541 tcgcccgccg tctcggtctg ccatttgttg ccgctccgac gatcgccgcc acctgcgcag
     3601 cctggacacc gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt
     3661 tcgacgacgc caattttatg gtgctggtgg aaccggagat tatcctcaat gcaccgcaac
     3721 aatatctgct ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg
     3781 ctccgcaacc agaaacgttg ccgctaaccg tgcgactggg gatcaataat gcgcaggcca
     3841 ttcgcgacgt attgttaaac agtagcgaac aggcgctgag cgatcagcaa aatcaacagt
     3901 taacgcaatc attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg
     3961 gtctgggcga tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg
     4021 tcctgccgca aaccgagaaa tttctccacg gcaccaaagt cgcttatggc attctggtac
     4081 aaagcgcctt gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt
     4141 ttcatctgcc gaccacgctg gcggagctgg aagtggatat caataatcat gcggagatcg
     4201 acaaagtgat tacccacacc ctgcgtccgg tggaatccat tcattacctg ccagtcacgc
     4261 tgacaccaga tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg
     4321 caaatttagc agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg
     4381 aaaaactctt cgtaggtcat cggcgtttga tcgggatggt taacccgcat atgttcgaca
     4441 tagttgtcgt aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg
     4501 gcttttgcca gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg
     4561 gatgcggcgt gaacgcctta tccggcctac aaatgctatc aacgcgctcg gtcagtcccc
     4621 tcgccccatc ggggagaggg ttagggtgag gaggaaccct cggcactatg ccaacattcg
     4681 ccaacatccc ccctcactct gactttagtg tgcgcctttt gcctgcgcca cgatctcctc
     4741 gacattttcc ggcattggct catacggcgt ttctttcgcc gttggcttcg gatctttcaa
     4801 tgccgccagt gccgtcttaa tcgagaacag cgccagaacc accacgacca ccataaagaa
     4861 gatggttaac ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt
     4921 atactgcgac ggaatattgc cgctgtcgat cattgcctgg aacttattag caatggccag
     4981 gaagccgact ttcgcatccg ggctaaacgc tttctgccag cctgcggtca gggtacaaat
     5041 cagcagccag gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa
     5101 caacaccacg gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa
     5161 cagcggccac agagtgttaa tgccgcccag cggatcgacc acgccctgat ggaggaagta
     5221 gccccacgcc agtacgcaca gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg
     5281 cttcaggccc ggagaaacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt
     5341 acccgcatct accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca
     5401 gaacgccaca tccatcatac cacccagcgc accgtgcagg atgtaagcca tccccaccgc
     5461 cagcgtcggc gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat
     5521 ctggttgagc gtatctggtg taatgctaaa gccccagcta ctcaccacct gcgcggcaga
     5581 agcgactaca tccgccgtcc ctgccggagc cagcaccgcc attgggctgt tcatggcaaa
     5641 atacacgccc ggatcgatga tacaggcaga aaccagcgcc ataatcgcca cgaaggattc
     5701 catcaacatc ccaccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg
     5761 cgtggtgccg gaagagatca gcgcatggaa gccagacacc gcaccacagg cgatggtgat
     5821 aaacaggaac gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttggt
     5881 cagcgcaggc atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat
     5941 agtcccgatt ttcaggaagg tcgagagata gtcacgcggg gccagcagca accacaccgg
     6001 cagcaccgcc gccacaaaac cgtagcccac cagcatccag gtcagctgca cgccggtaaa
     6061 gtcaaagtac ggtgcccagg tcggactttc tgccacccag ccgccagaga taatggcgaa
     6121 aatcaggaac accaggccaa tcactgacac ttcgccaatg cggcccggac gcagatagcg
     6181 caggtagatt cccataaaaa tcgccagcgg aatggtgaac gcaacggtat acgttcccca
     6241 cgggctatga gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat
     6301 gatcataaag caggccacca gcgctatcac cccggcggtt ggccccatct cttctttgac
     6361 cagctcaccc agcgatcgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg
     6421 caccgcaccg gcgagaacca ccccggcgag cagccagatc atccctggca ggtaacccat
     6481 ttgcgccgcc agtaccggcc caaccaacgg acctgctccg gcaatggccg caaaatggtg
     6541 accgaacagc actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc
     6601 tggcgtcata cgcgtcgggt caaccgccag cacatttttg gcgatataca gcccataaaa
     6661 acggtaagcg atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg
     6721 ttccccacga tttaaagcaa tgtatcccag agcaaatgct cccattacag agagcactgt
     6781 ccagacgagg tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga
     6841 tgttacattt tgtttctata acatctttgt acgatttaac aacacggtaa ccatgtaaat
     6901 cggagttgaa accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt
     6961 ttcctgcaat ctcatttaat tctgtcgggc tcatcccaaa actgccgtac ccagccgaca
     7021 agtgcagcaa cgccgcccct gttcatcgaa aacgacgatt tcccagctct gattttgccg
     7081 cccaagatgc agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg
     7141 ggtggcgtta agttccgtac ccaccacaca ctgcccgtca cgggtcatca taaatccggc
     7201 catcgatccc agcgtttcag ccagcgccgc tgacgcgccg ccatgcagca ggccaaacgg
     7261 ctgatgagta cgggtatcaa ccggcatttc ggcttccagc acatcatctc ccagacgggt
     7321 atacacaatt cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag
     7381 cgttaaatgg cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca
     7441 atatcctgta gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg
     7501 atctcttgtg gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag
     7561 ccgcgaatac gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg
     7621 tcggtggagc caggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg
     7681 acgctcaacg ccaggctttt cagcgccgct ttcgatgcgc cataagcact catgccaata
     7741 cgcggcgtgt gcgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga
     7801 aactggttca tggtttgctg gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc
     7861 tgctgccagt cctctttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca
     7921 ttgaccagca cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgcgacc
     7981 tgcgcagcat cggcaacatc catcacttcg gtcgcaaagg gatattgctc ctgagcgaac
     8041 gcttgatcaa aaccggtaac tttcgctccc gcctcaacaa acgccagcgc cgtggcgtag
     8101 ccgatacctt tacctgcgcc ggttacccag acatttttac cgctgaaatc cattatttca
     8161 cctcgcggga gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa
     8221 agtcgatatc accatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat
     8281 ccagaccgta gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg
     8341 gcaggataac ctcacgcagc gccgctttgc tggcggggac aggtgctggc agtaattctt
     8401 cagtcatcac cacccggcca gaccgcccgg ccacatattt cagcgacatc aaatgctcgt
     8461 cacggctgaa atcggccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg
     8521 cgtcggttgc ggtggtcata cagccaatgt gggcatatac cccggtaata atcagctggt
     8581 tacgtccact ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc
     8641 acttcaccag caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt
     8701 ccggcgaacg ggtcaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc
     8761 tctgctcttt cggctgggcg gtgtaataaa ccggaatatt gtgctgtttg cagtagtcgc
     8821 gcagcgcagc aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc
     8881 tgacaaaata gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc
     8941 agtcaacttt attcttaggg atatcgtgag actccggcag tgcgtaagcc tgtaattttg
     9001 gaatagccat cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat
     9061 tgttttttat cgactttccc gaccgccgtc agcggaagtg aatctacaca ctccacgcga
     9121 tccggtaatt taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg
     9181 cgcagcggct cttttaccac cagataagcg cagctttttt cgcccatcag ctcatcttcc
     9241 atgctcacca gtgcggcgta gatcaccgcc gggtggcgca gcagcaggtt ttcgatctct
     9301 tcggcagcga tcttctcacc gccacggttg atctgatctt tctcgcgccc ctgcacggtg
     9361 atgtacccct ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca
     9421 aaggcactgg cattgtgctg cggacttttg taataaccgc ggaaggtgta cggcccgcgc
     9481 gtcatcaggc gtccaacttc cccttgcggc agtggatttc cttcggcatc ggcaacccac
     9541 acttcatcgt ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca
     9601 tcaagacggg tgtagttcac cagcccttcc gccatgccaa acacctgctg caactggcag
     9661 ccaatctcag cgggaatacg cgccgcaagg gtggcagaaa gacgtgcgcc gccgacctgt
     9721 aacagtttca gcgaggcaag ctgcgcccgg ctttcacctt cggtcagcgc ctgcaaccac
     9781 aggctgactg ccggtggcac cagcgcggtg acgttcacct gatgtttttc aatcaatggg
     9841 aagcaaagcg tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg
     9901 cccagcgatc ccggcgaact catggcgtag ttatgagccg ccgggatcgc gcacaggtag
     9961 cgtgtctgtt gtgtgaactg acaaatctcg acgctacgac gcacgctgta gtagtagtcg
    10021 ttatgagtgc gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag
    10081 gccacttcat cagcaggtga tggcgtggca gtaaaatcct cagccggatg gttaatcgca
    10141 tcctgcaagt tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat ggaggaatgt
    10201 tctgtgacga aagtattgag gaaatcatcc ccgctaaaca gcgcatgttg gcgatcggca
    10261 atcagcaatg cgggttcaat ctggctggca taggcgttca gttcactacg ctgatggctg
    10321 aacagcgcca gcaccggcgc aacgcccagt ttcagcagcg cgaaaaaggt aatatacaat
    10381 tcagcgacgt tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgt
    10441 aaactacacg cgaggttatc cgccgcctga ttcagctccc ggtaactcaa ctgtcgctcg
    10501 ccgtcgataa ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc ggtcagcggc
    10561 aaatcctgcc agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg
    10621 aatggaatgc tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt
    10681 ttgacgcctg tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg
    10741 gcaaacagac gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg
    10801 ccgttacctt cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaatggc
    10861 tccagttcag caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtt
    10921 ggatgcagca gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag
    10981 ggagttgcga gatgccacag cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt
    11041 aactcactac tgcgttcgcg cagtacctct ttcatcgcct gagtcaccag ttcatgttca
    11101 tggcgatctt tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc
    11161 ggctgacgac gcgcggaacc ggctaacgga atggagctaa aacgctcgcc gtctttacgt
    11221 agcagcagtt ccgggctggc ccccagcagg acgccaccat cagccagcgg gacatggaag
    11281 ttgtaactaa ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg
    11341 gcgtcagtgg tgatatcaat caatcgtgac aacaccactt tgtcgacctg cggcgtggcg
    11401 gtaagtgcgg cggcgcgggc aaccatctgt tcaaacgtgg tttgttcagg aattgcctgg
    11461 cgttccacca cgttcagcga ctggctgcgg gtaaaacggc gggctgaggt ctgtttttcc
    11521 tggcgggaga acgactgcca ggattcggga atatacagcg acgaaggctg acgtggatcg
    11581 aagggaatcg ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac
    11641 agcgcggcga gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca
    11701 tcgaagcggg cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag
    11761 cgattgggcg caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata
    11821 tcatcctcca caaaatgata aaggctttaa taatgattat catttatatt ttcggtcgct
    11881 aacctaaaag caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg
    11941 ttgcatctgg ttgtggttac aatgaaaatg agaagcatta ttgatggatt cgcataagcg
    12001 caatgtgatg tcctgcgccg ttctgccccc tctcccttcc agggtgaggg ctggggtgag
    12061 ggttaatgtt cgcaccagtg ctggctgttc ccctcaccct aaccctctcc ccaaaggggc
    12121 gaggggacgg attgtgcgct ttgtcgaatt tgtcattacg cccttaacct tattaataac
    12181 aggaagctga tttgtgagac tcgccccgct ctaccgcaac gcccttctat taacaggact
    12241 tttactttca ggaatagccg cagttcaggc cgctgactgg ccgcgtcaga ttactgacag
    12301 ccgtggcaca catacactgg aaagccagcc gcagcgtatt gtttccacca gcgtcaccct
    12361 gaccggctca ctgctggcga ttgatgctcc ggtgatcgcc agcggcgcga ccacgccgaa
    12421 taaccgcgtc gcggatgacc agggcttttt acgccagtgg agcaaggtgg cgaaagaacg
    12481 caaactgcaa cggctctata tcggcgaacc gagcgccgaa gccgttgccg cgcaaatgcc
    12541 ggatctgatt ttaattagcg caaccggcgg ggattcggcg ctggcactgt atgatcagct
    12601 ttccaccatc gccccgacat taatcatcaa ttacgacgac aaaagctggc agtcgctgtt
    12661 aacgcagctt ggcgaaatta ccgggcatga gaaacaagcg gcagagcgga ttgcacagtt
    12721 tgataagcaa ctggcagcgg cgaaagagca aatcaaatta ccgccgcagc cggtcactgc
    12781 cattgtctat accgccgctg cacacagtgc caatctctgg acgccggaat cagcacaagg
    12841 gcagatgctg gaacaactcg gctttacgct ggcgaagttg cccgcagggt taaacgccag
    12901 ccaaagccag ggcaaacgcc atgacatcat tcagcttggt ggggaaaatc tggctgctgg
    12961 gttaaatggc gagtcactat tcctgttcgc cggtgatcag aaagatgccg atgcgattta
    13021 tgccaatcca ctgctcgcgc accttcccgc agtacaaaac aagcaggttt atgcgctggg
    13081 aaccgagacg ttccgtctgg attactacag cgccacgcaa gtgctggaac gacttaaggc
    13141 gctgttttaa gcattaactg tcagacgctg tcacctgcgg cggcgtctgg cgaaaacgtc
    13201 gcaactccac cagcaccagc aataacaaca cgccgataat caacaaacca aaaccgcttg
    13261 cgcttgcgga agcaaccggt gtcatcatcg cgcccaaacc acccagcagc gccgcgccta
    13321 tggcatcgcc cgtcacgttc tgcgccgtcc acaaaccgtt aatccgccct aacatcgctt
    13381 ccggggtttg cgtttgcagc attgtatatt gcagcaacga actaaccgcg ctcagccagc
    13441 cgaacagcgc cagacaaacc acgcctaaaa tccacatcgg catcaggcca aacagaccaa
    13501 tggcgaggaa cgatcccagc gtggagagca gcatcaataa ccctggtcgc gcactatgtg
    13561 ccagcttccc gctggttaac gcaccaatag ccgcgccgag cgggatcgcc gcgtagagaa
    13621 aaccaatctg tgccgctgac atctgccagt tgtcagccag cgccggatac agtacccgca
    13681 ccgcgctcgc catcgtcaat aaaccaccca gcagcgcaat cccgcccacc agcgggctgg
    13741 caagcagaaa acgaaatcct gccagtaatg atttcaacgg atgctcacgc ggctgcggtg
    13801 gcggtggcaa cgccggaagg cttaacaacg gtagcaaggt aataaacgtg cccgccgccg
    13861 ccagcccgta gttccaggct acgccaccgg tcgccagcaa taaaccgcca atcatgggcg
    13921 aaatcaccga ccccagacgc acggtcaaca tggtgatcgc cccggcctgc attaagtttt
    13981 cacgccctac cagtgcaggt gttgccgcca gtagcgccgt aacaccaagt gatgcgaaaa
    14041 aaccatccca taaaccaagt aaatagattg ccagcaatga cggctccggc agcagcgcgt
    14101 tcaggcacag tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa atcacttttt
    14161 tgcgttcata gcgatccgcc agcacaccgc caaccatcag gccaacaaac atcgcgccgc
    14221 cggtcagcgt caccgaaagc cccacctgcc aggtagaatg cgtcatcatc tggatctgca
    14281 ccggcaccgc gacgccgagc aaacccagag acacaattga gataaaacga gcgaggaata
    14341 ctgcgcgaaa cgccgggtgc gttttcaaca ggctgaggtt aagcagccag gattgtttat
    14401 tcattacaat gccttgccat caaatatttt aaatacctat tcttaggctg cacatgctaa
    14461 catatccaaa taagatcgat aacgataatt aatttcatta tcatggaagt tcgtatgtct
    14521 ggttctgttg ccgtgacacg cgccattgcc gtgcccggat tgctgttatt actgattatc
    14581 gcgacggcat taagcctgct cattggggca aaatcactcc ccgcttccgt agtgctggag
    14641 gccctctccg gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc gcgactgccg
    14701 cgcacccttg ccggtttact tgcaggcggc gcgcttggcc ttgccggggc gttgatgcaa
    14761 accctcaccc gaaatccact tgccgacccc ggcttgcttg gcgtgaatgc cggagccagc
    14821 tttgccattg tgctgggtgc ggcgctgttt ggttactcat ccgcgcagga acaactggcg
    14881 atggccttcg ccggggcgct ggtggcctca ttgattgttg cctttaccgg cagtcagggc
    14941 ggcgggcagt taagtccggt gcgtttaacc ctggcgggcg tggcgctggc ggcggtgctg
    15001 gaaggactga ccagcggcat cgccctgctt aatcctgacg tctacgatca actgcgtttc
    15061 tggcaagccg gttcgctgga tattcgcaat ctacatacct taaaagtggt gctgatcccg
    15121 gttctgatcg ccggagcaac tgcgctatta ctgagtcgcg cgctgaacag tttgagcctc
    15181 ggcagtgaca ccgcgacggc gctgggcagt cgcgtggcgc gcacacagtt aattggtctg
    15241 ctggcgatta ccgtgctttg tggtagtgcg acagcggtag ttggcccgat tgcctttatt
    15301 ggcctgatga tgccgcatat ggcgcgttgg ctggtgggtg ccgatcatcg ctggtcgctg
    15361 cccgtcacgc tactcgccac ccctgccctg ctgctgtttg ccgatatcat cgggcgggtg
    15421 attgtgcccg gcgagttgcg cgtttctgtg gtcagtgcgt ttattggtgc accggtgctg
    15481 atcttcctcg tgcgacgtaa aacgcgaggt ggtgcatgat ttacgtctct cgccgattac
    15541 tcatcacctg tttgctgctt atttccgcct gtgtggttgc aggtatctgg ggattacgca
    15601 gcggtgccgt cacgctggaa acctcgcagg tattcgccgc gctgatgggc gatgcgccgc
    15661 gcagtatgac gatggtggtc accgaatggc gtttaccacg cgtgctgatg gcgctgttga
    15721 ttggcgcggc actgggcgtc agcggtgcga tttttcagtc gctgatgcgt aacccgctcg
    15781 gcagccctga cgtaatgggc tttaacaccg gggcgtggag cggcgtgctg gtggcgatgg
    15841 tgctgtttgg tcaggacctg acggctatcg cgctgtcagc aatggtgggc ggcattgtca
    15901 cttcgcttct ggtctggctg ctcgcctggc gtaacggcat cgacaccttt cggttgatta
    15961 ttatcggtat cggtgttcgc gccatgctgg tggcctttaa tacctggctg ttgctgaaag
    16021 cgtctttaga aacggcgcta acagcgggtt tgtggaatgc cggatcgctc aacggcctga
    16081 cgtgggcaaa aacctcgcct tccgcaccca tcattatatt gatgctcatt gccgccacct
    16141 tgctggtgcg gcgaatgcgc ttgctggaga tgggcgatga taccgcctgc gcgctgggcg
    16201 tcagcgtcga acgttcgcgt ctgttaatga tgctggttgc agtggtgctt accgctgcgg
    16261 caacggcgct tgccgggccg atttccttta ttgctttagt cgcgccgcac attgcccgac
    16321 gcattagcgg caccgctcgc tgggggctga cccaggcggc gctgtgcgga gcgctgttac
    16381 tgctggcggc cgatctctgc gcccaacaac tgtttatgcc gtatcaactt ccggttggcg
    16441 tcgttaccgt cagcctcggc ggtatttacc ttatcgtctt gttaattcag gagtctcgca
    16501 aaaaatgacc gaatcagtag cccgtttgcg cggcgaacag ttaaccctgg gatatggcaa
    16561 atataccgtt gcggaaaatc tgactgtaga aattcctgat ggtcacttca cggcaattat
    16621 cggaccaaat ggctgtggta aatccacgtt actgcgtacc ttaagccgcc tgatgacgcc
    16681 tgctcacggg catgtctggc tggatggcga gcacattcaa cattacgcca gtaaagaagt
    16741 tgcacgccgg attggtctgt tggcgcaaaa tgctaccacg ccgggcgata tcaccgtgca
    16801 ggagctggtg gcgcgtggac gttatccgca tcaaccgctg tttacccgct ggcgcaaaga
    16861 ggatgaagaa gcggtaacga aagcgatgca ggccacggga ataactcatc tggcagatca
    16921 aagcgtggat accctttctg gcggacaacg ccagcgcgcg tggatcgcga tggtgctggc
    16981 ccaggaaacg gcaattatgc tgctcgacga acccacgacc tggctggata tcagtcatca
    17041 gattgatcta ctggaactgt taagcgaact gaaccgcgag aaaggctata ccctggcggc
    17101 ggtgctgcac gatcttaatc aggcctgtcg ttacgccagc catttgattg cattgcggga
    17161 agggaaaatt gttgctcagg gagcgccgaa ggagattgtc actgctgaac tgattgagcg
    17221 catttatggt ctgcgatgca tgatcattga cgatccggta gccggaacgc cgcttgtggt
    17281 gccgctcgga cgaacggcac cttcaaccgc aaatagttaa actaagtggt ctgccatcat
    17341 ggcatcctgt tttctggatg ccatcgcata gcgcaataac acgctaccac aagccaccat
    17401 cccgccgatc agcgcggaaa ggatcacaat aatcgcctta cccggaccgt cttttttcac
    17461 tggcaatgac gggcttaact gatatttaaa cggcgtaaaa ttcacatcgt tgatatttgc
    17521 ttttgttaac tgctcaacaa gatactgacg attacgtaat tcaccgttca gttccgcaac
    17581 gtcagtgacc gctttttcaa tttccagttt gcgttcaata ccgtctgcgc cgagagaaat
    17641 agagaaatcg gggtcatctt taacggcctg accattactg tatacgggct ttttaattcc
    17701 cgccgcgttg gcaatgtcga gtgaataatt gaggcgctga atgtttgcat caagttgatt
    17761 tttcgtttta atgcgatcct gagccagttt ttctttttca aactgggttt tgatctccag
    17821 tttattacgg acgttttcta tcgactcttt caccaccaac gcagagatat aatcgatata
    17881 cccgctcaaa acggtctgcg cctctttact ggttggcgcg gtaaaactta gcgtccagga
    17941 ggtatacagt gacggttcat cttttttctt actggcattg tcatcaaccg ctttcatttt
    18001 ttcgctcaat gcaacaatag cgcgatgcaa atccagttca tcgattttcg cctcttttaa
    18061 ttggtccatc acataaggtg atgaacgcag gtactcttcc agcaagctaa ccgactgaaa
    18121 cttcttgata aacaggttaa atgcttctgt acgatcaatt ttgatatcca gatccagcac
    18181 acgaagctta gtgaatgttt tctccaactc ttgccactga acaggttctg gaggcgtgac
    18241 aaccgccgcg ctggtccatt tttgcggcag gatgaaagag atcagcaagc ctgcgcaggc
    18301 aaacgcaaaa acgaccgcca tgaccgtttt tttggcccgc cataaaactg agattagatt
    18361 aagtaaatca atttcattat tactgggcga cgccagagga taatcgggaa aatgagcgtc
    18421 acttccctgt ttaatattca gtgatgacat ggggtataac ctgaaaatga gtccaatagg
    18481 gggaaatctc cacggcgctc actttaccag ttggtgagaa ttatccgaag ctgaagtttg
    18541 taaattcctt c
//
LOCUS       DAPGWD010000076        18526 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPGWD010000076 DAPGWD010000000
VERSION     DAPGWD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.076"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            294..545
                     /locus_tag="RHX88_002632"
     CDS             294..545
                     /locus_tag="RHX88_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231304.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRGNETIPDTLH"
     gene            complement(617..769)
                     /locus_tag="RHX88_002633"
     CDS             complement(617..769)
                     /locus_tag="RHX88_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HDV2231305.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATL
                     AYEVKR"
     gene            1061..2269
                     /gene="trbA"
                     /locus_tag="RHX88_002634"
     CDS             1061..2269
                     /gene="trbA"
                     /locus_tag="RHX88_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HDV2231306.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            2288..3358
                     /gene="trbB"
                     /locus_tag="RHX88_002635"
     CDS             2288..3358
                     /gene="trbB"
                     /locus_tag="RHX88_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HDV2231307.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNKETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESDHE"
     gene            3351..5642
                     /locus_tag="RHX88_002636"
     CDS             3351..5642
                     /locus_tag="RHX88_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HDV2231308.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELHPGEFFS
                     IFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     LTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(5679..8378)
                     /gene="nikB"
                     /locus_tag="RHX88_002637"
     CDS             complement(5679..8378)
                     /gene="nikB"
                     /locus_tag="RHX88_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HDV2231309.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVRHTLKSLGLGEHQYVSAVHTD
                     TDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFLQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(8389..8721)
                     /gene="nikA"
                     /locus_tag="RHX88_002638"
     CDS             complement(8389..8721)
                     /gene="nikA"
                     /locus_tag="RHX88_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HDV2231310.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            8955..9290
                     /locus_tag="RHX88_002639"
     CDS             8955..9290
                     /locus_tag="RHX88_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HDV2231311.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLIRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(9376..10224)
                     /locus_tag="RHX88_002640"
     CDS             complement(9376..10224)
                     /locus_tag="RHX88_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV2231312.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRIDKPEEAKRNITRLADRKIWERLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNACKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFNRSDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            10805..11056
                     /locus_tag="RHX88_002641"
     CDS             10805..11056
                     /locus_tag="RHX88_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2231313.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(11087..11368)
                     /locus_tag="RHX88_002642"
     CDS             complement(11087..11368)
                     /locus_tag="RHX88_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231314.1"
                     /translation="MRTAKPGKIPGFFASRSRENSMTLAERLIQKGFDEGFKEGFKEG
                     FKEGFKKGALEVAREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(11368..12270)
                     /locus_tag="RHX88_002643"
     CDS             complement(11368..12270)
                     /locus_tag="RHX88_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDV2231315.1"
                     /translation="MSKKKTTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTNPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGALEKALAIACQLQK
                     MGMTPEQIKQATGLSDDELKKITH"
     gene            complement(12335..12601)
                     /locus_tag="RHX88_002644"
     CDS             complement(12335..12601)
                     /locus_tag="RHX88_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231316.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(12695..13129)
                     /locus_tag="RHX88_002645"
     CDS             complement(12695..13129)
                     /locus_tag="RHX88_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HDV2231317.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(13858..14358)
                     /locus_tag="RHX88_002646"
     CDS             complement(13858..14358)
                     /locus_tag="RHX88_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDV2231318.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRLARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(14805..15980)
                     /locus_tag="RHX88_002647"
     CDS             complement(14805..15980)
                     /locus_tag="RHX88_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2231319.1"
                     /translation="MLRATKIRIYPTPEQAAFLNAQFGAVRFAYNKALHIKKHAYQRH
                     GVNLSPRNDLKPLLSVAKKSRKYAWLKEFDSMALQQAVINIDVAFSNFFNPKLKARFP
                     TFKRKHGKQSSYHCVGIKVLDNAIKIPKLAPIEARLHRELHGKLKSITVTRSATGKYY
                     ASILCDDGASAPEKPTYLKEEKITGLDMGLEHYAITSDAVKVPNPRHLINVSRNLRRK
                     QKALSRKQKGSANRKKARIRLAALHERVANARADFQHKLSRAIVDENQAVIVETLKTA
                     NMMKNHNLARAIGDAGWHSFITKLEYKAATKGVHLVKLDQWFASSKTCHCCGYKMPEM
                     PLHKRIWRCPECGAEHDRDINAALNIRQKGILELKAAGLVVSAHGGQRKSVAQTVAA"
     gene            complement(16110..16829)
                     /locus_tag="RHX88_002648"
     CDS             complement(16110..16829)
                     /locus_tag="RHX88_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDV2231320.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQIRALEQWMIPNKLENAI"
     gene            complement(16826..17260)
                     /gene="psiB"
                     /locus_tag="RHX88_002649"
     CDS             complement(16826..17260)
                     /gene="psiB"
                     /locus_tag="RHX88_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDV2231321.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(17315..>18526)
                     /locus_tag="RHX88_002650"
     CDS             complement(17315..>18526)
                     /locus_tag="RHX88_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231322.1"
                     /translation="VGADAFSPDELRTDLFSDDEGGYVDCVALDAALLEKLQAVAEHL
                     REAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEEERLNELMTRYDALENQC
                     EESDLLEAEMKLMRCMAKVRAWTPEMRTGSGVVVSWRYGNVCVQRGVQLRSEDDAADD
                     ADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPDKSLALLAWTLCLNVFGS
                     GAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARLAALLPEGWSRDMTTFLS
                     LSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAIGFHMRDWWQPTKANFFG
                     HLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKDNRWVPGWMCAPHPQTDA
                     TERTDNLADAA"
BASE COUNT         4402 a         5066 c         4688 g         4370 t
ORIGIN      
        1 aacttatggc atgttgcatt aatcaggtag aacaaattat catttttagc gtcagttata
       61 gacagctctt ttctggtcag agtcatcaac tttgtagcgt aacagtaaaa aaagccggcc
      121 tcaccagtcg gtttttttac gtcttttgta tctgcaaaat ccgccccgcc atgcccgtca
      181 caccggggaa actcatgctc tgaacctgaa ccagccatct caactttgcc gtcttctgaa
      241 aaaactggca cattcagttc gataactacc cataaataca gaaagaaaat ccgatgtcag
      301 aaaagtggag taaagaattc gactcttgct ttcaggtggg gagcatagaa gaaattgccg
      361 atgctctcga gaaacgcagc ggaagcgagg aaaacgcgtt agccatgctg gcaatgacag
      421 ccttcacact catgacccga cgtggtatct gcgaaatgca gaacgtggcc cccgatggca
      481 aaggcattcg gctggagcta atcggcttca ggggaaatga aacaataccg gatacgcttc
      541 attgaagaag aaagggcgca atgaggtcag ccttcgcaac aaagcggcgg cagaaaacct
      601 gccgccgtgt tgcccgttaa cgtttaactt cgtaggctaa cgttgccaca agcactgtgt
      661 ttccctgctg gagccggagt acgcaaagcg aatccctcac catccagaca aaacacagaa
      721 tcgtcacaca gatgacgatt aacatcatta aaaacgttcg ctgtggcatc atagcccctc
      781 cattgcctta cggcggttat cgggctacta taatgttgtc gaagcattac agtagagccc
      841 ttggttgatt gataaaaaaa tctccagggg ctttctgctg tataccgtcc acaaaacgct
      901 ttcggcatac agcctcaagc gcccgcagct attctaacga ctttcgtatc tgcaacaatc
      961 cttttatcac acggctaatt cctctggtta gctcttatcg cctgcaaata aatttttaac
     1021 ctttccgttt gatttaaatt ccagaaatgg agactttgtc atgtcttaca atcgacagcc
     1081 tgtcgcagaa gatcctatgc agatatgggg cgctgttggt gtccttctta tcctgctgct
     1141 attcgttata tggttattct tgccggaagt ggtttacgcc agctgcctga ttcttcatac
     1201 cttatgggga ctggttgact ggggaccatt tcataactat gcagcaccac gatacaacct
     1261 gcttgccatg accggtaaca acgccgccaa cataagctat agccagtggg ttaacgtcat
     1321 ggagcagacc ataggtattc tgtggatgta cctcttacct gtgacactct ggtgtttgtg
     1381 ggagtggtat caacatccgg gacaaagtcg tttcacccgc agaccagtag atattacccg
     1441 cctgccacac atctttgctt cgttgtcccc tgctatcgcc cctgtactgg cagatggtga
     1501 tccggaaaaa ctctttcatg gtggcaaacg acctgaacgg cgggttgctc tcaccccgga
     1561 agcatttgtc gagcagcata ctctcatcac caatatgcag ctcgatgttg cagctgcaag
     1621 acgatgtttc atggcccagt taggtaaacc tttaacgtcc tggaaagaca tggcaccaca
     1681 tgaaaaagcg ctctttgcca tatttggtct tcagtatttc ctggatgatc gtaaggcagc
     1741 attaaagctc atggatacac tgaacctctc atgcagaatt aaaagcaaac gggacagtgg
     1801 gaaattctgt acccctgttt attcactggc caaatcagct ttccagagag tgattaaaag
     1861 caacggagca cagcaatggc tgaaacaaca tcgctacgtc cgcagtgggc tggtctggct
     1921 gtatgcacat gacctgcggc ttacgccccc caactggatt tggctgaaag gcgtggacag
     1981 gacgctgttt tatgcattac accgcgccaa taccacgaaa ggatttattg aaggcgctgg
     2041 cgtggtggcc gttgcacgtg cagaggccga agccatgcgt tttggcctcc cctgccctga
     2101 accctgtgtt gatgaagcgg tggaaggctt acggcgggat atgctcagtc tcggtcttat
     2161 ctgggatgaa ccacaaccag atcgcgaccg gaaacgccgc atcctgacca actggtccct
     2221 caccgacgac atcctgcccc gcacacctgc tacagacaac gaattctgat tatccggaga
     2281 actgaaaatg acaatcgagt actttgcaac acgtattgca aaacatattt ccgtgcaggc
     2341 aatctggccg gatggcagga cagaaattat tgccgtcctt ccacgggatg ccctgtcagg
     2401 gctggtaaca cgaaataatc aactgattca tatagccttt gacgccatgg gcacccgtaa
     2461 caaagaaacc gtcctgattg ataaacagga gcataacgct gaccagatac ttacagctat
     2521 tgattcctgc ctgcgccttg aatatctgat ggaacgccgc ttcagcagaa cacctttgtt
     2581 ccgtggcata atcgccagcg ttgtgctgtt cgtaatggca acgattggct tctctctgtt
     2641 ccgctatgtt gatcgtgtat tctgggatga tacaacgcct gaagcagtac agacagcagg
     2701 tgagccacga ctgcttcccc ctcacctgaa tcacactgtg ccattaaatg aaggcattca
     2761 actcccggtt cccccgaaaa aagacgtcca ggtaccggag aaaacaatca caagcaaaaa
     2821 tccggaagcc gcagctgcgc gacataatct ggctactgtt ctgaagcgga acgccgatcg
     2881 gggcatgttt accatcaacc tgtcatccgg tcatgaacga accctgttcg catttctcga
     2941 tcctgcctgt cctaactgca gacttctgga gccggcctta aaacgcctgg ccagcgactt
     3001 caatgtcgtt atctacccgg tttcagttat tggtggagag gaatccaccg accgtgtggc
     3061 accactgctg tgtgaaaaag atgctcagaa acgtgctgcc ggctggcacc gactgtattc
     3121 cgcagataac ggaatgatga cgccatcaga agaaacaacg cctgcagatg aaacatgcct
     3181 gaaagcagcc agagctgcga ttgatgttaa caatgtcgcc ttccgtaagt tcggatttgc
     3241 cggtacccca tgggtgctgt ccgacacggg ctggcatctg cctactggaa ttctgcagga
     3301 aactggcacg cttaatttat tcctgaaaac caccgacagc gagtctgacc atgagtgaac
     3361 atcgcgttaa ccccgagctt ttacaccgta cagcctgggg aaatcctgtc tggaatgcac
     3421 tccagagtct caatatctat ggcttttgcc tggtggcaag tcttgtggcc agttttatct
     3481 ggccacttgc attaccggca tgtctgttgt tcacactgat aacgatgctg gtatttagtc
     3541 tgcagcgatg gcgctgccct cttcgtatgc ccatgacgct ggaatgtgct gacccgtcac
     3601 aggacagaat gattaaaaga agccttttca gcttctggcc aactctgttt cagtatgagg
     3661 tcatcctgga gtcaccggcc agcggcatat tttatgttgg ttatcaacgc gtcagggata
     3721 taggtcggga gttgtggctg agtatggacg atctgacccg ccatataatg tttttcgcca
     3781 ccaccggtgg tggtaaaacg gaaaccatct ttgcctgggc gattaacccg ctttgctggg
     3841 caagaggatt cacccttgtc gatggcaagg cacagaatga tacggcaaga acgatctggt
     3901 atctggccag acgctttggc cgtgaagacg atgtggaagt catcaacttc atgaacggag
     3961 gaaaatcccg cagtgaaatc attctgagtg gtgaaaaaac acgacctcag tcaaatacct
     4021 ggaatccctt ctgttacagc actgaggcat tcacagcgga gacaatgcag tcaatgttgc
     4081 cccagaacgt ccaggggggc gagtggcagt cccgggccat tgccatgaac aaggcactgg
     4141 tatttggtac taaattctgg tgtgtgcgcg aaggcaaaac aatgtcgctg cagatgctcc
     4201 gtgaacatat gactctcgaa gggatggcca aactttactg tcgtggcctc gatgatcagt
     4261 ggccggaaga agctatcgca ccgttacgta actaccttca ggacgttccc ggtttcgacc
     4321 tgtcgctggt caggacacca tcagcctgga cggaagaacc cagaaaacaa catgcttatc
     4381 tctccgggca gttttctgaa actttcagca cattcacgga agcattcggg gatatttttg
     4441 ctgaagattc cggtgatatc gatatccgtg acagcattca cagcgacagg attctgatgg
     4501 taatgatccc cgctctggat acatcagcgc atacaacgtc tgctctggga cgaatgttta
     4561 tcacccagaa aagtatgatt ctcgcccgtg atttgggcta ccgcctcgaa ggaactgatt
     4621 ccgatgcact ggaagtcaaa aaatataagg ggcgtttccc ttatctgtgt ttccttgatg
     4681 aagtgggggc atactataca gaccgtattg ctgtggaagc cactcaggtg cgttcactgg
     4741 attttgctct gattttgatg gcacaggacc aggaacgtat agaaggacag acaacagcca
     4801 cgaatactgc aacactgatg cagaacacgg ggacaaaatt tgccggcagg attgtcagcg
     4861 agggcagtac ggccagaacg ctgaaaagcg ccgccggtga agaagccagg gcgaggatga
     4921 acaacctgca gcgtcaggac ggcatatttg gtgaatcatg gattgacagc ccacaaatca
     4981 gcatcctgat ggaaagtaag atagatgttc aggagttgat tgaacttcat cctggcgagt
     5041 tcttttctat tttccgggga gaaaccgtac catcagcatc attcttcatc cctgacaatg
     5101 agaaaagctg cagtagcgat cccgttgtta taaacaggta tatcagcgtg gatgcacctc
     5161 gcctagaccg tctccgccgt ctggttcccc gtacagctca acgtcgaata ccgtcaccgg
     5221 aaaatgtcag tgccatcatt ggcgtcctga ctgcaaaacc ctccagaaaa agacgaaaaa
     5281 ttcgcacaga gccgcacacg atcgtggata cattccagca acgcattgca ggacgtcagg
     5341 cggctatggc aatgctggaa gagtacgaca cagatatcaa cgcacgtgaa agtgccttgt
     5401 gggaaacagc ggtaaatacg cttaaaacga ccacacggga agagcgacga attcgctata
     5461 tcacgctgaa cagaccagaa ctacctgaaa caaaagagga aaatcagata tcagtgagag
     5521 cagaacgagc aggtataaac ctgttaacgc ttccacagga caataaccac ctgacagggc
     5581 gtcctgtaaa cgggtttcat cataaaaaaa ccaaccgtcc ggactgggac ggaatgtact
     5641 gaacagtggc cgcgccggat tctggcgcgg cctttttttt aacctggaga aggcggttcc
     5701 cagcgtttat cctgctccgg cggaggcagt gatgcgtcat cgttgctgtt cgccgcgata
     5761 taatcgcggt agtagcgttc acttccttca cggtgatgat ccacgtccgg tcgcgtaatc
     5821 ctgtagtctt cgtgaccggt acggcctgtc acattatgcc aggcaaccat ttcagcaaag
     5881 acctggttaa attgtttgtc gtttccttct ggttcaaaac ccatgcgtgg atcacgcccg
     5941 aataataccg gtgcaatcaa atccagttta tctgccagcg cattgcggtc atggtgatta
     6001 cggaaaacaa ccctgtcacc ataatccgta cagacaaact ctcccgtccg acggtcacgg
     6061 aaacgaacac tcccgttctt ctgcaaacgg gcctcgagat caccggtatt accgtatacc
     6121 ggcgttccgc ccggttcaca aagcacgaca cagcggtctg ttgtcgttgt gcgtgactta
     6181 tctttacggc gatcgcgata atcccagcct ctcagctgcg atacagcagc cctgtctccc
     6241 tgagcagcct gaatttcgac ccactgacgg taagagggtg gataccactt cccgtcagca
     6301 ataagtttct gccgctcatc cctgatgtct tctttcagcc tgatcaatgc ctgcattcgc
     6361 tgaacttctg caatgtgata atgcagctta cgtaatgccg gatcatcgta ctgcgcacga
     6421 atgtgtgact tccgcagacg gcatgcctga tgaatttccc gacaacgttc cccataacgc
     6481 agatctggtt tacgccattg ttcacgccat gccagatagc gggcacgcag gtcagcacgg
     6541 gcctcagctc tggcttcacg tcgctgccgg cgcagcattg ggtcacgttt actgctgacc
     6601 ccgtacctgt cactgacagc cagctccggg ttatagcgac tttcaggttg caccctgtca
     6661 aacaaatctg ctggggcact cacaaaaggt ccggcctgtg gctctgcacg ccccagagtc
     6721 agatcaggat gaatgctgct ggccttcacc ggcgtttgct catgattaaa ggcatcaacg
     6781 acaacaagcc cgtgatgttg tttctgtagc atcagtccct gttgtgcaaa caactcatga
     6841 caacgctgcc aggtaaactc accctgtgtg atcatttctt caatctgagg gctacaatgg
     6901 ctgatgctgt atccccacag actttctttg tcagaaaaca gttcgcgttt gatagccccc
     6961 tgcggccccg tacctgtacg catacggtgc cattctttat cggtcgcagg acagctctcc
     7021 atacgacgac cacggaagcg ttctgttggc gtaacctgga gaaaaatatc tgttggtaca
     7081 ggctgccagc caccatcgag ttgattcaca ttgtcgagcg tcagcagtga ccatacgctg
     7141 tcagcacgaa cgggagtctg gttatgatca taaccgtcac agataaccag gtgaccatgc
     7201 tgaacacgca tccataatcc cgcttcagcc agtgtgcggt gaatagcctg gcagttctcc
     7261 agccgtcctt cccgtgccat ttccggcaga cgttctccaa gatgacgaca ggcatactgc
     7321 tgcagacttt ctgtttcagc gattttttcc gggcgaacat cagctgcaat gaagtccgga
     7381 ttatagcgtc ctggtgcttc cacctggctg aaaatgtctt ttggtactgg cgtgtagtca
     7441 cccatttttt tcatgagttt ttctgcacac caggaagggc caaacgagtc aagctgtaca
     7501 ccttccctgt tgcgatccca gccatccatc accagaaatt ttccgtccat ctgagacagg
     7561 tacagaccat cttcagcaag acgacgatgc agggataacc agtcatgtac aggttcactg
     7621 cgtaaaccag ctaccgctgt ctgcgcaaca tactcgcgaa aagtttgttt ttttccgcgt
     7681 gtccaagcgt tctggcgatc acgttcaacg gcagttttgc gaacgatacg atttcccggc
     7741 gcatggaccc agcaaccgtt atccggagca aaaccatgct tcagttcaag ctcacggcag
     7801 gcacggctga gtttttcctg actccatgaa aggcaattga gataacctgt aaccggatgc
     7861 acccggttga ccgcaacgtg aacatgcagg ttatcagtat cggtatgcac ggcagagaca
     7921 tactgatgtt cacccagccc gagagatttc agggtatggc gtacactgtc ataaatctgt
     7981 tccggacgcg ggctttcatg ggcctgccag gaaagaatgt agtgaaaaac aggatcagta
     8041 ttatctttcg catagtgtgc ctgctgtgca atgtactcca tatctgcagc ggcagtttca
     8101 agcgatgtac agttgtgaaa acaggtcaca ccataaaagt taacccattc gcagccgtcc
     8161 ttcatgacat caaccagtgc cacgaatgat tcatttcgaa ggcgtgttgc ataatcaaca
     8221 agacgactga agcggctccg gtgcggttgt tcagcctgcg aagaagaaga taaattcagt
     8281 tcctcatcgg tcatgtcatc ccggacagag acgtaggaca ccaggtcttc gaacgaagac
     8341 ttgccgtccc ttctcttttt cggaatgact gcattcaccg gagaatcctc aaccttccat
     8401 taactgttta cgcaatgtat ttgtaagttc ggttatggca acaagtactt ctgcgtattc
     8461 cttgtcaccg gttctttttc cctgaacaaa cagatgcttc tgcatccttc ccagtctcat
     8521 taactccttc agaaaagcat catctgtccg ggagttaatt cgcttattca gtgctgcatt
     8581 tcggatgtaa gcagatacag taagtccgga atcttcagct tttttcctga tagtttcatc
     8641 ctcaacagga gagaatctga gtgtcctgac aactgtcttt tgccgtacct cacttttctt
     8701 tcttaccgca ctatcactca cttcaggctc cttacggggt gtcggggcga agccctgacc
     8761 agatggtaat tgtaatagcg tcgcgtgtga cggtattaca attacacatc ctgtcccgtt
     8821 tttcaggcca ttatagcctg ttatttttgt gcatcatagt cttatgcaga cggcagaggc
     8881 agggcgcagc cctgccagga gtcagttttt gatgataaaa tcagacttca acatgaaacg
     8941 gagagaaagt gccaatggct tctaaaaaat tttactcaga tgatgacatc cagctggcaa
     9001 aagctgcttt atcggaattg ccagatctca ccgctcaacg gaaaacattg cgtgattttc
     9061 ttgatgccat tcgtgatgac attattgtcc tgataaggac aaagggttat acactggcag
     9121 atgttcgtga cacgctcaaa aatgcagggt atgaagtagg agagaaggca cttcgtgaca
     9181 ttattcggga agcagaatcg aaaaaaccat cccgccgttc ttcctctaaa acagcaccta
     9241 aaaagacaga ttcaggcagg aaagatggca tcgatatgaa caacaattga tgtgacaatg
     9301 tcacatcgtg ttctgagatt atgatgatta cagaaaaaaa agccccggca taaataccag
     9361 ggcttttctg tttaatcaga ctcgtggcgg caatacacct ggataatgcc gtgcaataat
     9421 atcattcagt ttttcaacaa ggtcagaacg attaaagaca atgtgtacgg tccctttctg
     9481 gtagccacgc accgtaaaat attccccttc taacagctta ccgacattgt cgccgttccc
     9541 gagaaattca ccatactgcg ccccgatgct gttcctgaag tcaggaacgt tgcgcccctc
     9601 cagcagataa aacggacgta ccaggtcatc cagtgcctcc cgtccgctgc agtccagcgt
     9661 cacacgcccg ttactccagc ggtacagcag gttttcaata atgattttct tacccagacg
     9721 gcagggatta ttggttctgt aatcccatga caatttccgg tagacatcaa tcagtccctg
     9781 ttcaaatgtc tgcatcttgc aggcattcag atggcggaat gttgccagta cattgtccag
     9841 ggtgatttca ggacaggtgt cgctcatcag ctggctgttc cactcatcac gctggcatga
     9901 actcatgaag gtgtacatgc ccgtatctgt catcaggcgc tcccagattt tccggtcggc
     9961 aagacgggtg atattccttt tcgcttcttc cggtttatct atgcgacgaa cggaatcagt
    10021 cacgcagtct tcaaacccgt aaagaaaaat atcacctgac gcatcgagca gaatcttgcg
    10081 cgctgtcgcc aggcattcca tcgcctgcat gaacagtgca ataccttcgt tacgctgcgc
    10141 gatgatgcgg tcgatagcca cagacggaat aacatctgtc gtgccggcag tattcaggtc
    10201 agcagtgggt aaagtctgat tcatcacatt gtctcctcat cgtcacgact gccgggtcag
    10261 ccaggtcatt tcccggagga gcgaagctcc ttctgccgtc cgggtcttcg gggatttccc
    10321 cgcagtggac ctttccgacg ggggacgctc accacgtcac ggggcagttg tccgggggca
    10381 gacgcaaaag tttttgacgc ctgcggcaaa aagttttgtg gctggtacat ctctcccctg
    10441 gacgactgaa acgggatgtg gtcatgtatg tacccccgga aagtccgtct gcggagaaca
    10501 ggccgcagtg cggcggcaga caggcagaga cagctgcgcc atcaggcgca gtggccttac
    10561 acaccgtaag gtgtgcaggg agcgccgcat acggcgcgac tgaagaaaaa cgacggcaca
    10621 acaatggcat gtggtggtca ctggcacacc actcccgcac aggtgaaaac agagaaaacc
    10681 gcacgacatt tacaagcaaa aaaaagtgaa atttcttcat ttttttacac ttatttatcg
    10741 cgctgatttg gcaattgtga tgataaaata aagtgaagtt ttttcacttt ttcaaaggtg
    10801 ttttatgacc ggctgggaac tgcgaatctg gcgaaaaagc atgctctggt cacgcgaaaa
    10861 agcggccaga gaatttggcg tgacgcagag aacatggcac gcctgggaaa atgcggaaca
    10921 ggtggacgtc accgtctgga ggaccaccca ggcactttca gtccgtgacc tgctgcctca
    10981 catgcagggg atgcgtaagg cagatatcat caggcgactg gaaaatgaac tgggggaaac
    11041 ggcagggaat gtgtgagcgt gattcacagc ctggctgaat gccaggctac tgctcatcag
    11101 gaaacagctt tttcagttct tcaccggaaa gtccggtcgc ctcctgaatc cgttccggcg
    11161 tccagcccat atcccgcagc cggcaggctg cttcccgcgc cacttcaagc gcacctttct
    11221 taaaaccttc tttaaaacct tctttaaaac cttctttaaa accttcgtcg aaacctttct
    11281 gtataagacg ttctgcaagc gtcatactgt tttctctgct cctgctggca aaaaagcccg
    11341 ggattttgcc gggctttgcg gttctcatca atgagtgatt ttcttcagtt cgtcatcgga
    11401 aagtccggta gcctgcttaa tctgctccgg cgtcatcccc attttctgaa gctggcacgc
    11461 aatagccagg gctttttcaa gcgcaccttc catcctgccc tcctggcgac ctttttgctc
    11521 aagacgttct gcgatagtca tcagtttatc ctcatgctgc ggcagacgct cagccagcgc
    11581 ccgcataaat tccggtgata cagagtcccc tgcgttaacg atgtaatgaa tcagaacacg
    11641 cacctgctcg tctgaaattt ccaccatgac ctgcgggagt ttgtccagca gctccatcat
    11701 gtcacgatgg cggatatgct tcatcagcag cgtcagtgcg gccatccggc gatggttcat
    11761 gatttcattg tcatcaacga cagtgatatc aaccagcgga aacggatttg tgtatatttt
    11821 agccgcaagt gccggattct caaaacagtc cagccagttc atgctgtacg gatacgggct
    11881 gcgctcaccg tgatagaaca gcaccggaat caccagtggc aatgttttgt gctgctccag
    11941 atgtctctgc atagccgcca cactgtagcg taacatgcga aaaggcatgt acagattttc
    12001 ggtgctctgg tgctcgatga gcgtataaac atagccgtct tcacccccgg tggttttcac
    12061 gctccagagg atatcgctgg cgtactgatg caggtccggc tcaacaaagg tggcgggttc
    12121 cagcttcagc gtggacaggt cgcacaactg ccggtactcc gcaggaagat gcagttccag
    12181 aaaatccctg gcaacgtcgg gattcgccag gaacgaccgg aacgcggcat catgcggcgt
    12241 gggcgtcgtg gtggttttct tcttactcac ggttcctcct ggtgagatgt ctgaacgtat
    12301 cctgccgcct gcacaggcag acggcaaggg ttaatcagtg gcagacccgc tcaatttcaa
    12361 gcgcgcggag caggtggccc cattcgctga ctttcatggc ggggatacgt acccgcttct
    12421 gctcactgca gcgtctggcg atatgaccgc tcatcgccag gacgtccggg ttaatctcat
    12481 cctgtgccag ctcctgacgt ctgaagcggg tatggcggtg caggggttta ttctcccgca
    12541 ggcactcttc acgcagacgc tttacatact cggcggttgc gaagataatt gatggtctca
    12601 tggcataatc tccatagata gtgattgaga tgccggactt cctggcgggg aagcgccggc
    12661 ggagagaggc agggtttatc gccctgcctt tttttcattt cggacagcga cgcagtgcca
    12721 gcgcgagcgc tttcatcttc cagccttccg gcgtgcggta catcaccttc cagtagccgt
    12781 cgatgaacac ataatggaag cgcacctctt cagtgctttc agtggcaata gcctccctga
    12841 aagaacggta ctcacgcgcc tcactgtcca tccggaaacc actgtcacgt ccgtaataag
    12901 ccgtcacacc gtccaccggg ttctgaagcg tatgcccggc gggtttgtcg caacggggac
    12961 cgaggcaacg gatatcaccg tgttcaacca gggcttcggc ctgctgaccg gtggcatagt
    13021 gggtatgcag gatgcgaccg ttccaggtcg gctccccgtg caggtggcag taaacggtac
    13081 ggaacacccc gtcgcggcaa aaaacggaaa tcgtgctgct gactgtcatg aaaacgtctc
    13141 cttctgctga atcaggtcgc cgggccgacc ggttgctccc cggaggggcg aaagcccctt
    13201 ctgccgtcca cttcttcggg gtttctcccc gcagtggacc tttccgatgg ggcgtccgtc
    13261 accacgtcac ggggcagctg tccgggggcg gacgcaaaat tttttgccgg gtttatggct
    13321 aaaaattttg tggacggtat atcttcccct ggactgctga aacgggatgt ggttactcca
    13381 gcccccggaa agtccacagc gggaaacagg ccgcaggggt ggcggcggaa gggttgactg
    13441 cgccatcagg cgcagtggcc ttacacaccg tcaggtgtgc agggagcgcc gtttccggcg
    13501 cgacaggggc ggcgcgaccg gaacggtcgc tgcaagcctg gtttttcaga catcggaccg
    13561 tagcggcagc ccgaaaccgc gccagcgggt tcggcggaga ctggcgggtg cgccagcagc
    13621 tgcctgtcgg agtcgggcct gacgaccgga tgccgccagg cagccgggag ccgaaggtgt
    13681 ccccgcctcc ggaaatcaga acaacagaaa cgacataaca gaaaaatgcc tcacaccatg
    13741 acgggcagga agggcagcgc cgcaggcgcg gccctcctgc atttcaggca ggcgtaagcc
    13801 tgccagggtt ttccggcggc ctggcaggcc atacccccgc cggagcgggg gagtaaatca
    13861 ccgacggaac acatgaccgt caataaaggt gaaggagtcc aggaataaat cccgcgcata
    13921 cgcctcatag tcaaaataga gcgccaccgt ctccggcacg tcagccagca ggccggtatc
    13981 actggcgaac tcaaccgcaa aagcctcctc actgtcagcc tcgccccacc aggcatcgcg
    14041 gaaggtgtca aaatccgtct caccggtatc ctccacccag agacgataag cctcttcgca
    14101 gccttcatcc cgtgccaggc ggaagccttc aacccaggcc cagttgatat ggcattcaga
    14161 ggccatattc cccgggaatc cctcataatc ctgaaacatc agttcaggat cggcttcatc
    14221 ctggtgaaga gcacggcagg cggcgaaaaa gtcgcgctca tcatcaaacg tggtcaggtc
    14281 aaaccagcgt ccggcgatgc ttccacagtt gtatttgtgc caggttccaa cgtatacagc
    14341 aggtgcaaca acagacattc ccgaatcctc catagtcaca tgttctgcga caccgggcgg
    14401 tgccgtgcct ccataaaagc cgggcgataa ggcggggatg caggggcgca agggaagccg
    14461 cagcctgacg cacacggctg acgggagttg cagggtgagg ccgcgaaacc tgagcggacg
    14521 aacgaccgga aaatcacggc agacgggaag gagggaccgg gtgaccgcag cggcgaagcg
    14581 tacccttgca tccctgccag cccggctaag atggaggcac ggcacagccc gcacacgacg
    14641 ctgaggccac agagctgaaa ccccacaagg ccatgccccg catggccggg taaaagcgga
    14701 gccgccggcg acttccttat tccggcgagt gcagaggaca cgccgcatga cctccagacc
    14761 acccgtgact gctccccgcc ctgtcgggcg aggcttccca cttctcaggc cgcaaccgtc
    14821 tgtgcgacgg atttacgctg gcctccatgg gcagagacga cgagtcccgc cgcctttagt
    14881 tccagtattc ctttttgccg gatgttgagt gccgcattga tatcgcggtc gtgctctgct
    14941 ccacactcag ggcatcgcca gatgcgttta tgaagtggca tttccggcat tttgtaacca
    15001 caacaatggc acgttttcga actggcaaac cactggtcga gttttaccag gtgaacgcct
    15061 tttgtcgctg ccttgtactc cagctttgtg atgaagctgt gccagccagc atcacctatc
    15121 gctcttgcca gattgtggtt tttcatcatg ttggctgttt tcagcgtctc cacaattacc
    15181 gcttggtttt cgtcgacaat cgcgcgagag agcttgtgtt gaaaatcagc acgggcattc
    15241 gccacccgtt cgtgtaaggc cgcaagacgg attctggcct ttttacgatt cgcgcttccc
    15301 ttttgcttac gggataatgc tttttgtttg cgtcgcagat tacgacttac attgataaga
    15361 tggcgaggat tcggaacttt tacagcatca gaagtgatgg catagtgctc cagtcccata
    15421 tccagacctg taatcttttc ttccttcagg tacgtgggtt tctctggtgc ggaagcgcca
    15481 tcgtcacaaa gaatggaagc gtagtatttc cccgttgcag aacgggtgac agtgatgctt
    15541 ttcagctttc catgaagttc acgatgaagg cgtgcttcta tcggggccag tttggggatt
    15601 ttgatggcat tatccagcac cttaatcccg acacagtgat agctcgattg ttttccgtgt
    15661 ttgcgtttaa acgtcgggaa ccgcgctttc agcttcggat taaaaaagtt ggaaaatgcc
    15721 acatcaatat tgattaccgc ctgttgcaat gccattgagt caaattcttt gagccaggca
    15781 tatttacggg actttttggc cacagacaac aacggtttca gatcgttacg cggacttaaa
    15841 tttactccgt gtctctggta agcgtgtttt ttgatgtgaa gagctttgtt gtacgcaaaa
    15901 cgtaccgcac cgaactgggc gttgagaaat gcagcctgtt caggagtagg atagatgcgt
    15961 atttttgttg ctcttaacat agtcaccgct cattggtaca ggtttttatg ctaacaccgt
    16021 aataatattt ttaaagccct tcgggctttt ccgcctttcc tccccacccg cattgggcga
    16081 gggttctcgg cggcttttcg ctgatttcat catattgcgt tctccagctt gttcgggatc
    16141 atccactgct caagtgcacg tatctgtacc ggtgcaccac gaccggcctc accggcttca
    16201 aaaatcagcc gccagagtgg ttcctcctgc cagaaaaacc ggtcaaatga tgacagtgat
    16261 ggaaaacgcc cgcaccaccc ccagaaaatc gtttccagat catgctcctc aacaacacca
    16321 gaccagcggg acaaccagct tccgaccgtg gcgggagtat gaaacgcaag gtcaatctgc
    16381 gcctgagcaa aacatgtctg gtattccagt tcccgtcgtt ctgcctcacg ttcccgctgg
    16441 cggctgtatt tctgctctgt cagcgtggtt ttctgacgac gccgctcacc ttcacgaaaa
    16501 ggaacggacg ggaacagacg atgcccagca tcggatggca ggggaagaac acagacttca
    16561 ccaaaacttt caatcagttt atccacggca gccagccaca ggagacggtt gccatgaaat
    16621 tcctccttcg tcagcgcagg agcaaaaaag cggagatcgg tcagcgaaat ccggcggctg
    16681 ccattcaggc agcgaaagaa agcgctggca taaggccatg ctgaaagtgt gcgtccctga
    16741 cgacgacgtt gttctgtgac agccacagcc tgcatggcgg cctgttgctc tgcgctgagg
    16801 gggatcagtg cccgtgaacg tgcgctcatg cctgacctcc ttctgccatc aggatatgga
    16861 tgatgtcgtt aacactgtac ccctgcgcat caagtgtcgc ggcaagcgcc agcgtgtgac
    16921 tgatagcgac aggcgtgaag atatgttgca cctgaacgac ggcgaaaggc tgtccaccac
    16981 aattcaccag aaccagcatc cagagctgag agacgtctcc cggcgaacat aatgccaggt
    17041 ggaaacgttc gtgggctggc gtgaaacgaa cctggacggg gaaaaagccc ccgaactcac
    17101 tgccatactc gccattcatc atccagttag ccggtgcgtc cacttcacgc atcacctcgt
    17161 gagtgagatt acggcgcata tcgcttcccg cagcacggat atcttcatat tcctgtgcgt
    17221 tcatggactg cagggcattc agggtcagtt cagttttcat cacggtatct cccttgctgc
    17281 cgccccgtct ccggcggggc ggtgtggttg ttcatcaggc ggcatcagcc aggttatcgg
    17341 tgcgttcagt ggcatctgtc tgtggatgtg gagcacacat ccagcctgga acccagcggt
    17401 tgtctttcat atggtgctct gcatgttcag ccgcatcacc tttcttcatc ttctccgcgt
    17461 cccgtgcggc accggacagt ccggcctcat tcagggctgc gataatttgt ggctttttca
    17521 ggtgtccgaa gaagtttgct tttgtcggct gccaccagtc gcgcatgtga aaaccgatgg
    17581 cgctctccag cgtatccagc gggctgcgtg acgtgtgacc acactcgcgg gtctgaacgc
    17641 cgtggatgct gcacgcggtg cagaaactga gcagggataa cagcacctcc tggctgagcg
    17701 acaggaacgt cgtcatgtcc cgtgaccatc cctccggtaa cagggccgca agacgggctt
    17761 tttctgccat cagcgccatg aacgcggcac cttccttccc tgacggcgca tcgctggtca
    17821 gcgaataatg tttacattcc aggctgattt gtgctggttt actgtacgct ccgctgccaa
    17881 acacattcag gcagagcgtc catgccagca gtgccagaga tttgtccggt tgctgcatga
    17941 gtgccgcctg gactgccagc gtgcgttcgg aagacatttt cgtcagcagt ggcagactga
    18001 tttcctccac tgacgctttt tcctgcacct gttccgtgcg gtcagcgtcg tcagccgcgt
    18061 catcttcact gcgcaactgc acaccacgct ggacacacac attaccgtaa cgccaggaca
    18121 ccaccacacc gcttccggta cgcatctccg gcgtccacgc tctgaccttc gccatgcagc
    18181 gcatcagctt catttctgct tccagcaggt cggattcctc acactggttt tccagcgcgt
    18241 cgtaacgcgt catcagttcg ttcaggcgtt cttcttctgc ctccgtcagc accgcttccg
    18301 gctccggcag acagcggtat gttccggcat cctcacggca ctcaccgaca ggctccatgc
    18361 gcccggcgca ccattcccag ccttcggctt cccgaaggtg ttcagcgaca gcctggagtt
    18421 tttccagcag ggcggcatcg agcgccacgc agtccacata gccaccctcg tcgtcgctga
    18481 acaaatcggt gcgcagttcg tctggcgaga aggcatcagc ccccac
//
LOCUS       DAPGWD010000077        18365 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPGWD010000077 DAPGWD010000000
VERSION     DAPGWD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.077"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            62..668
                     /locus_tag="RHX88_002651"
                     /pseudo
     CDS             62..668
                     /locus_tag="RHX88_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            771..1274
                     /locus_tag="RHX88_002652"
     CDS             771..1274
                     /locus_tag="RHX88_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDV2231323.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1341..2798)
                     /gene="nuoN"
                     /locus_tag="RHX88_002653"
     CDS             complement(1341..2798)
                     /gene="nuoN"
                     /locus_tag="RHX88_002653"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDV2231324.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2805..4334)
                     /gene="nuoM"
                     /locus_tag="RHX88_002654"
     CDS             complement(2805..4334)
                     /gene="nuoM"
                     /locus_tag="RHX88_002654"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDV2231325.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4498..6339)
                     /gene="nuoL"
                     /locus_tag="RHX88_002655"
     CDS             complement(4498..6339)
                     /gene="nuoL"
                     /locus_tag="RHX88_002655"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDV2231326.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6336..6638)
                     /gene="nuoK"
                     /locus_tag="RHX88_002656"
     CDS             complement(6336..6638)
                     /gene="nuoK"
                     /locus_tag="RHX88_002656"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDV2231327.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6635..7189)
                     /gene="nuoJ"
                     /locus_tag="RHX88_002657"
     CDS             complement(6635..7189)
                     /gene="nuoJ"
                     /locus_tag="RHX88_002657"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDV2231328.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7201..7743)
                     /gene="nuoI"
                     /locus_tag="RHX88_002658"
     CDS             complement(7201..7743)
                     /gene="nuoI"
                     /locus_tag="RHX88_002658"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDV2231329.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7758..8735)
                     /gene="nuoH"
                     /locus_tag="RHX88_002659"
     CDS             complement(7758..8735)
                     /gene="nuoH"
                     /locus_tag="RHX88_002659"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDV2231330.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8732..11458)
                     /gene="nuoG"
                     /locus_tag="RHX88_002660"
     CDS             complement(8732..11458)
                     /gene="nuoG"
                     /locus_tag="RHX88_002660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDV2231331.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11511..12848)
                     /gene="nuoF"
                     /locus_tag="RHX88_002661"
     CDS             complement(11511..12848)
                     /gene="nuoF"
                     /locus_tag="RHX88_002661"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDV2231332.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12845..13345)
                     /gene="nuoE"
                     /locus_tag="RHX88_002662"
     CDS             complement(12845..13345)
                     /gene="nuoE"
                     /locus_tag="RHX88_002662"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDV2231333.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13348..15150)
                     /gene="nuoC"
                     /locus_tag="RHX88_002663"
     CDS             complement(13348..15150)
                     /gene="nuoC"
                     /locus_tag="RHX88_002663"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDV2231334.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15244..15906)
                     /gene="nuoB"
                     /locus_tag="RHX88_002664"
     CDS             complement(15244..15906)
                     /gene="nuoB"
                     /locus_tag="RHX88_002664"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDV2231335.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15922..16365)
                     /gene="nuoA"
                     /locus_tag="RHX88_002665"
     CDS             complement(15922..16365)
                     /gene="nuoA"
                     /locus_tag="RHX88_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDV2231336.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16996..17934)
                     /gene="lrhA"
                     /locus_tag="RHX88_002666"
     CDS             complement(16996..17934)
                     /gene="lrhA"
                     /locus_tag="RHX88_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDV2231337.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4590 a         5093 c         4722 g         3960 t
ORIGIN      
        1 ataatgataa aataacgtct tttattgata atttatatat gaaaaaaaat ccttttgtta
       61 tattatttct gatttcactc cctctgatta tttttaccaa ccctgggtca ctgcattatc
      121 gccaacgccc cgacacgtca gtcctgagca attagaaaaa acagttcgtt atcttacaca
      181 aactgtgcat ccacgtaatg ccgacaatat tgataatctg aataggtccg tagagtatat
      241 aaaagagttc tttgtcagta acggtgccag agttaccgcg cagggcgtcc ccgattggct
      301 attacgactc tgcgcctggt agccaggatt acccttaccc gcaatgtcct ggctttatcc
      361 cgatcggggg gactttattg cctggtcggc agaatacagg atatcaacgc cgttcgtcag
      421 gtaaaaacgg cattgttatc atcccgggat ttatctgttt attctatgaa tgccccatgg
      481 tttattcccg gtattgattt ctctgaccac ctgaattatt ggcaacacga tattcccgcc
      541 gtaatgatta ctgacaccgc tttttatcgt aataaacaat accacttgcc cggtgataca
      601 gcagacagat tgaattatca gaaaatggct cagatggcgt tataaccttg ttacacaaca
      661 gtaaataata aaaatttctc gaggcgggtc cagaaacagc gacatgtaca ttttattttc
      721 cctcgtctgt tttaagatgc gaaacataaa attcaatgga ggaaaaataa atgggaatga
      781 ttggttattt tgcagaaatt gactcagaaa agattaacca actgcttgag tctactgaaa
      841 aacctttgat ggataatata catgacacgc tttcaggatt acgccgactg gatattgata
      901 aacgctggga ttttttacac tttgggctga caggcacctc tgcttttgat cccgcgaaga
      961 atgatccgct gagtcgtgcc gtgcttggtg aacacagcct cgaagatggc atagatggct
     1021 tcctgggatt gacctggaat caggagttag ccgcgacaat cgaccgactg gaatcactgg
     1081 atcgaagtga gctgcgtaaa cagttttcca ttaagcgact caatgaaatg gagatttatc
     1141 cgggagtgac cttttcagaa gagttagagg ggcaactctt tgcttcgatc atgcttgata
     1201 tggaaaagct gattagcgcg tatcgacgta tgctgcgaca agggaatcat gcgctgacgg
     1261 ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg ccgaacatgt cggcggcttt
     1321 ctgacttaca aactgatagc ttacatcagc ggcattgcta accgcacaat gctaatcagc
     1381 ggttgtggcc atacacccag caccagtacc aacagtgcag agatcagcac cacgataccg
     1441 cctgcgctgt actgccagtt tgacggtgca tcgcgacccg gttgttccgg ggcgtgcagg
     1501 tacaggctca ccgccacgcg caggtagtag taaaggccga ttgccgaacc gacaaccacg
     1561 gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg ccagcacgta gaacttacca
     1621 ataaagccca gcgtcatcgg aataccggcc agagacagca tcatcaccgt catcaccgcc
     1681 gcgaggatcg ggcgatgcca gaacagaccg cggtaagaga acagggaatc agcatccggg
     1741 ccacggtacg ggctggacat caggctgacc acgccgaacg cgccgaggct gctgaacaga
     1801 taaccggcca ggtaaactcc taccgcttcc atcgacatct cgcccgtttg cagcgcaatc
     1861 agcgccacca gcagatagcc gaggtgagag atagatgagt aaccgagcag acgtttgata
     1921 ttggtctggc tcagcgccat caggttaccg aagatgatag aggcaaaggc gataatcgcc
     1981 agcactaccc gaatcgcttc gctgtcaccc accggtgcgt agaggaacag acgcatcacc
     2041 acaccgaaga tagcgatttt gctcgccgtc gccaggaaag tggaaaccgg cgcaggcgcg
     2101 ccctggtata cgtctggcgt ccacaggtgg aacggcacca gagagagttt gaagccgagg
     2161 ccaacaatca tcagtccgaa acctgccagc aacagcggct cgttgagcat accgtcgccg
     2221 aggtttttac ccagcgcgac aaacgacagg tcgccagact gtgcatacac cagcgccata
     2281 ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg tgtatttgat actggcttcc
     2341 agcgaacgtt tctggcggaa agcgtaaccg accaggccaa acagcggcaa agagatcagt
     2401 tcaataccga ggaacaaaga cgccagatgg ttggcattcg ccagcaggat cccgcccagc
     2461 gcggcaatta acaccaacag gtagaactca tccttgttgt cgttatagcc ttcaagccac
     2521 gggtaggcga aagtacaggt ggcgaggctc gccaacaata ccagcccggt gtaaagcatg
     2581 gcgaaaccat caacgcgcat cagcggcgta acgtccatag cgcccgcctg gccgacaaac
     2641 cagagcgaaa ccagcgccgc gttaagccca ataaccgaga gcgtagcgtt gaggaaatga
     2701 ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg tcaagccgac gatcagcaac
     2761 ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg cgatttacgg ccttgtagta
     2821 gtaacggaat taacaaacca ctgctggata ttgccaatcg cggagtgcga ggtatccaga
     2881 atcggctgcg gatagaagcc cagcagtacc agcagcacca ccagcaacag gatcataaac
     2941 agctcacgca gcgacatccc tggcagttcc tggctggcaa tctggctttt cgctttaccg
     3001 aagtaagcgc gatgtaacat cgccagcgaa taaacagatg caaagaccag cccaaaggta
     3061 gagataacgg taatcaccgg gacaacctgg aagctgccga acagaatcat aaattcgccg
     3121 acgaagttac cggtgccagg catcccaagc gttgccaccg caaagaacag cgacagtgct
     3181 ggcagccatt tcatcttgct ccacagaccg cccatcatgc gcatgtcgcg ggtatggata
     3241 cgttcataaa gctgaccaca aagaataaac agacccgccg ccgacaaacc gtgcgcaatc
     3301 atctggatta ccgcgccctg gtaggccaac tggctgccgg tgtagatagc aatcagcacg
     3361 aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt tgatatcggt ctgggcgaag
     3421 gccatccacg caccgtagaa gatgccgata acacccagcc acatagcaat tggcgcgaac
     3481 tctgccgacg cgttcgggaa cagcggcagg gagaaacgca gcaaaccgta agcggcagtt
     3541 ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg gagcctggga gtgcgcatcc
     3601 ggcagccagc catgcagcgg aaccaccggc attttgactg cgaaggcgat gaagaagccc
     3661 agcattaaca ggtattccac accactggac attggcgtat tcagcagctc ttcatagttg
     3721 aaggtccaga cgccggtcgc attgtagtga acaaaaacca gcgccaggat ggcaatcaac
     3781 atcaccaggc cactcgcctg ggtgtaaatg aagaacttgg ttgccgccgt gatacgcgtt
     3841 ttaccgtcag aggctttatg cccccacagt gcgatcagga agtacatcgg caccagcatc
     3901 atttcccaga agaagaagaa caggaacatg tcgatggcaa ggaacacgcc gataacgccg
     3961 cccaggatcc acatcaggtt gaggtggaag aagccctgat atttttcgat ctctttccac
     4021 gaacagagta ccgccagcac accgagcaga ccggtcagca cgaccatcag cagcgacaga
     4081 ccgtcaatgg cgagatgaat agagatacca aaacgcggga tccacggcat gtcgaattca
     4141 gactgccact gcggaattcc ggcggattgc gtcagtgaat aaccgccctg caaccacagt
     4201 tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg cgatccagcg cggcaccttg
     4261 acgccaaagc gttcggtctg ccagcacagg aagccgccaa taaagggaat taatattagc
     4321 cagggtagta acatggcgat ctttattcct tgtaaaagtc ccgtcaggac cggattttca
     4381 acgaattctc acgacagaaa attctcttcg gactgggggt agtgtcggat gcgccgcgaa
     4441 ggcagcttat ccgacctacg ttcttatatg cccaggggca atcccacaat ccttaactca
     4501 acgcagtacc atcaacagtg ccagcaccac gaccgcaccg atgctcatgg atgccacata
     4561 ccagcgcaga taaccgttct cgcttaacag cagaccttta cctgcaaagc gggaaagaac
     4621 cgccgggatg ttcatcattg agttcagcgg atcgcgtttc agcaaccagg caatacccag
     4681 gaacggcttg acgaacactt tgtcgtacag ccagtcaaag ccccaggcgt tgtaccacca
     4741 ggtactcagc aggcggcccg gcgcactgtt ggcgatggag gtcaccagag tacgtttacc
     4801 cagccacagc caggctgcca gcagaatgcc gaccaccgcg accacgccag aggtaatttc
     4861 cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc ggaagcacgc cctgcagcgg
     4921 cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc agcacaatca gcggcaggct
     4981 gtgagttacc cctttcacgg cgtgagcgtg aatttgttct tttccgtgga agacgatgaa
     5041 aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg accagacctg ccaccatcag
     5101 attgatatga ccattcgcca tcgcacccgc gaggatctca tccttactga agaagcccgc
     5161 agtgaccagc ggtagtgccg acagtgctgc gccgcccacc aggaagcaga gataaaccag
     5221 cggaatagat ttacgcagac cgcccatctt gaagatgttc tgttcgtgat ggcaggccag
     5281 aatgacggaa ccggatgcca ggaacagcag cgctttaaag aacgcgtgag tcatcaagtg
     5341 gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg aacatgtagc caatctggct
     5401 catggtagag taagcgagaa cacgtttgat gtcggtctgt accagcgcgg caaaaccggc
     5461 cagcagcagc gtaaccgccc cgacaatacc caccagatgc agaacttccg gcgtcatcag
     5521 gaacaggccg tgggtacggg cgatcaggta gacacccgcg gttaccatcg ttgcggcgtg
     5581 gatcagcgcg gagacaggcg tcgggcccgc catcgcgtca gcaagccatg tctgcaacgg
     5641 caactgcgca gatttaccga ccgcaccgcc cagcagcatc agcgtcgccc acatcagcat
     5701 gttattgccg tcagcaaagt gcgctggtgc cagttccacc atttcgcgga agttcagggt
     5761 gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg aaaacgtcac ccacacgggt
     5821 cacgacgaac gctttcattg ccgctgcgcc attcttcgga tcggtgtaat agaaaccgat
     5881 cagcagatag gagcacaggc ccacgccttc ccagccgagg tacatcagca gcaggttatc
     5941 ggcgagaacc agaactacca tgctggcgat gaacaggttg gtgtaggcga agaagcgaga
     6001 gtagccctct tcaccgcgca tgtaccagga ggcgtacatg tgaataagga aacccacacc
     6061 agtgaccacc gagagcatgg tcagcgacag gccgtccagc accaggttaa aaccgatgtt
     6121 aaagtcgcct accgacatcc acgtccacag cggctggctg tatgactgct cgccgttagc
     6181 gaagaaatca acgccgataa aggcggttac cagcgccgcc aggcccacag agccaacgcc
     6241 gacgatcgcc gaaacgtttt cagaccagcg cccacgggag aacgccagca ggacgaagcc
     6301 aatcaatggc aaaataatgg ttaaggcaag catgttcatc cgcgcatctc acttactgaa
     6361 tcgatgttca ggttctggcg acgacggtga agttgcagca gcagcgcaag gccgatactc
     6421 gcttctgccg ccgcgaggct gatggcgaga atgtacatca cctgaccgtc ggtctgaccc
     6481 cagtagcttc cggcgaccac aaaggccagc gcggaggcgt taatcatgat ttccagacca
     6541 atcagcataa acagcagatt gcgacggata accagaccgg ttaagccaag aacgaataag
     6601 attgccgcga ggatcagtcc atgttgtaag gggatcatgc gtgctcctcc gtttttcttt
     6661 tcgcgctgtc gtctttacga ttgctcagca cttcacccgc acgctcttca cgaccgacgt
     6721 ggaaggccac aaccagaccc gcgagcagca gcatagaagc cagttccacc gccagtacgt
     6781 aaggcccgaa cagcgtaata cccactgctt tagcactgat tggcgtaccg tcgataccct
     6841 gatcattaac accaaggatg gcgtaaacaa tcaccaccag catgatggcc gacaaaattg
     6901 ccggaccaat ccacacctgc ggtttcagcc actggcgttc ctgttcgatt tctgaaccgc
     6961 ccaggttgag catcatcacc acgaacacga acagcaccat aatggcaccc gcgtagacga
     7021 taatttccag cgcaccggcg aagtaagcgc ccagtgagaa gaacaccccg gaaatcgcca
     7081 gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt ggtatgggtg atcactcgca
     7141 aggtcgcaag tatggctatc aggccacaga tataaaaagc gaactccatt gcccctctcc
     7201 ttacggtaac aggctcttga cgtcgatagg cttggcttcg ttctctgctt cgcccttatc
     7261 tttgccgtcg attgccatac ctgccatccg gtagaagtta tattccgggt atttgcccgg
     7321 accggagatc agcagatcct ctttttcgta aaccagatcc tggcgcttgt attcccccat
     7381 ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag gcttcttcgc acagaccaca
     7441 gaaaatgcag cgtgagaagt tgatgcggaa aaattccggg taccagcgac cgtctttggt
     7501 ttctgctttt tgcagcgaga tacagccgac cgggcaggct accgcgcaga ggttacaggc
     7561 tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg atacgaccac gataacgggg
     7621 cggcagatag accggctctt ccgggtacat tcgcgtttcg cgtttggcga acgcgtgcag
     7681 gccgatcatc cagatactac gaacctgggt gccgaaacct actaacaatt cttttaaggt
     7741 catggtctta ttgcccctta ttgcgcctgc cagagaatga cagccgccgt taccagcaag
     7801 ttgatcagcg tcagcggcag gcagattttc cagccgaagg acattacctg gtcataacgc
     7861 ggacgcggta acgacgcacg aatcaaaatg aacatcatca taaagaacgc ggttttcagc
     7921 gcgaaccaga tgaatggcgg taacaacggg ccttgccagc caccgaagaa cagcgtcacc
     7981 atcaatgcag agatggtcac aatcccgatg tactcaccca cgaagaacag accgaacttc
     8041 ataccggaat attcaatgtg gtaaccatcc gccagttcct gctcggcttc cggctggtca
     8101 aacgggtgac ggtgacatac cgccacgccc gcgatggcaa aggtaataaa accaaagaat
     8161 tgcgggataa cgttccacac atgcgcctgg ctgttgacga tgtcggtcat gttgaatgaa
     8221 ccggcctgcg ccaccacgcc catcagggaa agcccgagga acacttcgta gctcagggtc
     8281 tgcgcagaag cacgcatcgc acccagcaac gagtatttgt tgttacttga ccagcccgca
     8341 aacagcaccg cgtaaaccgc cagacctgcc atcatcagga agaacaaaat cccgatgttc
     8401 aggtcggcaa ccacccaacc cggactgact ggcacaatcg caaaggccag cagcagcgag
     8461 gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat ccgagaattt cgggatccag
     8521 tcttctttaa agaacatttt gatcatgtcc gcaaccagct ggagcgaacc gccccagcca
     8581 acacggttag gtccgtaacg gttctggaac agacccagca ggcgacgttc gccaaagctc
     8641 atgaatgccc cgcaggtgac aaccaccagc aggatcacca ccgctttgag gatggtcagc
     8701 aggatctcaa tcagttccgg tgatatccaa ctcattgttg tgcctccttg agatcctcaa
     8761 gatgcgcgcc agccagcacc ggagcaatac cggacatacc catcggcaag cccacctgcc
     8821 ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac cgtgttgcca tcgtaactaa
     8881 aggagacgtg tgtacctgcg ttcacaccta acttcgcggc atccgctggg ttgagtttga
     8941 tgtacggctg cggcatacgg ctctggaaga ccggagcacg ctgtgacaat tcatcgctgc
     9001 caaacaggtg gtaatacggc gcgatacgcc atttcccgtc ctgcggctgg aagcgtgccg
     9061 gtacgctggt gaagtaatcc agaccatttt cgctggtttc aaacagacgc acgcccggat
     9121 cgccaaagcg cagtttgccg cccacttcgt cctggaattt gttccacgcc tgcggggagt
     9181 tccagcccgg cgcccaggca aacggcactt gcgaacggtg cgcggtcggc tggttgttac
     9241 cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg ctgacgcggc tcatgaacgc
     9301 tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg cggttcacgg gccagtttct
     9361 gcccacgaat acggaatgtc gcatccggcg cagcatcttt gatacctgcc agttccggga
     9421 ttttcgccac aacagcgtca atcacatggt cgagctgcgt ccagtccact tcacggctca
     9481 gcagggtgct gtgcagcgag tgtaaccagc gccagctttc cagcatgaca gttttgctgt
     9541 cgtaataagc aggatcgtaa acctggaaga aacgttgggc gcggccttcg ttgttgatca
     9601 ccgtaccgtc gctttcagca aagctggcag cagaaagtac cagatgggcg ttttccataa
     9661 tcgctgtgcg ttgatgatca accaccatca ccagcggcgc tttagccagc gcagcattca
     9721 cgcgggtagc ggaagcgtga cgatgcagat cgttttccag caccaccacc gcgtcggcgc
     9781 gtccggtttc cagttcggtc aacgcttctt caagcgaacc gccacccata atgcccagcc
     9841 ccatgctgtt gacggaacgg gcaatcatgg tgataccaac gtcagcgccg cgacctttca
     9901 gggctttcgc gacgttagcc gcagcctgaa tcacctctat gctaccggcg ttcgtcccgg
     9961 agataatcaa cggtttcttc gcacctgcca gtgcctgcac gatgacgtcg attttgcttt
    10021 gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt atccagcgca tgggcgatgg
    10081 caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta agtccacgcc gcgatatcat
    10141 ccagacgggt gtcatcaacg ttggtaacaa acagcggatg cttcgcacgt tgaccgatgt
    10201 tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc tgccgccatt tcgcgcgctt
    10261 tacctttcac agcctgacgc actgccagcg cgacgcgcgc gccggtctgg gtaacgtctt
    10321 cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg cagagccgga gtataaatgc
    10381 cgccttcacg cagcactttc agcgccagtt gcagacgttc ctgctcaccg tgagcgatac
    10441 cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc aaagttgctt tccacgctgg
    10501 cacgcggaga accaataccg atcactttct tcgactgacg cagaatatct gccgcgccct
    10561 gcattgcctg ttcggcgttg agggtaatga aatcatcgcc acgacgctgt actggttgac
    10621 gcggacgatc tttcaggttg acgtaaccgt aaccgaaacg accacggtcg cagaggaagt
    10681 agtggtttac cgtaccgttg taacggtttt cgatacgacg cagttcgccg taacgttcac
    10741 cggggctgat gttacagccg atggaacatt gctggcagat gctcggcgca aactgcatat
    10801 cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt aaatacgccg gtcgggcaaa
    10861 tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc gtcttccggg cgaccgaagt
    10921 agacgttgtc gtgcgcaccg taaacgccca gatctgtacc gtcagcgtaa tctttgtagt
    10981 aacgcacaca gcggtagcag gcgatgcagc ggttcatttc gtgagagatg aatggcccca
    11041 aatcctgatt acggtgggta cgtttggtga aacggtagcg acggaagctg tgtccggtca
    11101 tcacggtcat atcctgaaga tggcagttac cgccttcttc acataccgga cagtcgtgcg
    11161 ggtggttggt cattaaccac tcgaccacac tttcacggaa ctgtttcgct tcttcgtcgt
    11221 caatggaaat aaaggtgcca tcggaagccg gtgtcataca ggacatcacc aggcgaccac
    11281 gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg gcggcaagca ccgacgcttc
    11341 ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc cagagacaga caagcttcca
    11401 gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc tacatgaatt gtagccatta
    11461 gcatgcttcc agttttctcg gtcagggacc gagcgttaat cgaaattcgg ttaccagcgc
    11521 tctttcagca ggttcggctg aatcccatta atcaaatggg tattgctgaa cggctgtttg
    11581 attcccgcct caaattcttc gcggaaatat ttgatggcgc tctgtaacgg ctccactgca
    11641 ccaggtgcgt gggcacagaa agttttaccc gggcctaaga atcgacacag ttgctcaagt
    11701 gtttcgatat cgcccggctg accttcacca cgctccagcg cacgcagaat tttcacgctc
    11761 cacggcagac cgtcgcggca cggcgtacac cagccgcagg attcacgggc gaaaaactct
    11821 tccaggttac gcaccagcga caccatgttg atctcatggt caaccgccat cgccagcgcc
    11881 gtacccagac ggcttcccgc tttaccgata ctttcgaatt ccatcggcag atcaaggtgc
    11941 gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc aggctttaaa tttcagacca
    12001 tcacgcatac caccggcgta atcttcgagg atctcgcgtg cggtggtgcc gaacggcagt
    12061 tcccacagtc ccggattttt cacccgaccg gagaagccca tcagcttggt gccagcatct
    12121 ttacttttcg agatgttctg ataccactcc acgccgttag cgaggatcgc cggaacgtta
    12181 cacagggttt cgacgttgtt gacacaggtc ggtttgcccc atacgccgga ggttgccggg
    12241 aaggggggtt tcgagcgtgg gttagcacga cgcccttcca gggagttgat taacgctgtt
    12301 tcttccccgc agatgtagcg ccctgccccg gtatggacga acagttcgaa gtcgaaacct
    12361 gttcccataa tgtttttgcc aagcaagccc gcttcagtgg cttcggcaat ggcgcggcgc
    12421 agatttactg ccgcttcgat atattcgcca cgcaggaaga tgtagccacg gtaagctttc
    12481 agcgcaaacg cggagatgag cataccttcc accagcaggt gcggcagttg ctccatcagc
    12541 aggcggtctt tataggtgcc tggctccatt tcatcggcgt tacacagcag gtaacggatg
    12601 ttcatggatt cgtctttcgg catcaggctc catttcaggc cggtagaaaa gcccgcgcca
    12661 ccgcgccctt tcagaccagc gtcttttacc tgattaacga tttcgtccgg agacagcccg
    12721 gtcagcgcct tacgcgcgcc ttcgtaaccg tttttgctgc gatattcgtc cagccacact
    12781 ggctgtttgt catcgcgcag acgccaggtc agcggatgcg tttcgggagt acggataatg
    12841 tttttcattt ataccgctcc agcagttcag ggatcgcttc cggggtcaga tgcgcgtgag
    12901 tatcctcatc gatcatcatg tttggccctt tatcacagtt ccccaggcag caagttggca
    12961 gcagcgtaaa gcgaccgtca aatgtcgttt gccctggttt gatgtttagc tttttctcga
    13021 gcgccgcctg aataccctgg taaccgttga tatgacagac cacgctgtcg caataacgga
    13081 tcacatggcg gccaaccggc tggcggaaga tctggctgta gaacgttgcc acaccttcga
    13141 cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg gatcgcacca tccggcaccc
    13201 agccacgctg cttctgaacg attttcagcg cttcaatgga cgccgcacgc gggtcttcgt
    13261 agtggtgcat ctcgtgctca atcgcttcac gctctgccgc actcagctca aaagcctcgg
    13321 tttgtggttg ttgattctcg tgcataatta gcggtccaca tctgacataa caaaatcgat
    13381 actgcccaga taaacaatca ggtcagacac caggctgccg cggatcgccg ccggaatttg
    13441 ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga taactcatgg tgctaccgtc
    13501 gctggtcagg tagtaactgt tgatcccctt ggtcgcctca atcatctgga aagattcatt
    13561 ggcaggcatc accggccccc acgacacttg caggaagtgg gtgatcaggg tttcgatatg
    13621 ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg tgatccgctt tgaacgggcc
    13681 ttccggcatg ttgttgaggc actgctcaag aatgcgcaga ctctggcgca gctcttccac
    13741 tttcagcatt acgcgggtgt agcagtcaga aacaccacca ccaaccggga tttcaaagtc
    13801 gaagttttca tagccagaat aaggacgcgc cttacgcacg tcgaagtcga tcccggtagc
    13861 acgcaggccc gcgccagtgg tgccccactc cagcgcctct ttcgcgccat aggcggcaac
    13921 gccctgggaa cgacctttca gaatggtatt ttgcagcgcc gctttctcgt aagacgccag
    13981 acgtttcggc atccagtcga ggaactcacg cagcaggcga tcccagccgc gcggcaggtc
    14041 gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata cggaaacccg tgatcgcttc
    14101 caccagatcg taaattttct gacgatcggt aaaggcgaag aacacgggcg tcattgcgcc
    14161 gacgtcctga ataaaggtcg agatgtacag caggtggctg ttgatacgga acagttcgga
    14221 gagcataacg cgaatgacgt taacgcgatc cggcacggtg atcccggcca gtttctctac
    14281 cgccagcacg taaggcattt cgttaacgca gccgccgagg tattcaatac ggtcggtata
    14341 cggaatgtag ctgtgccagg actggcgctc gcccattttc tccgcaccac ggtggtggta
    14401 gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg agttgtaaaa cgatacggaa
    14461 agccccgtgc gccgacgggt ggttcggacc gaggttgagg aacatgaagt cctcgttttc
    14521 ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc agggcttcca tctccagatc
    14581 ctgtttggct ttggtcagct caaacggcga gaattcggta gcgcgcgccg ggtaatcttt
    14641 acgcagcggg tgacctttcc aggtttgcgg catcatgatg cggcgcaggt tcgggtgacc
    14701 gtcgaaagta atgccaaaca gatcccaggt ttcacgctca taccagttag cgttcgggaa
    14761 cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc agcgccacct tcagcatgat
    14821 gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg gaaaaatccg cggcaggtaa
    14881 cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt aagtcaaaca gcatgacgta
    14941 aggtttcggc agtttcttta agaaatcgcc aacttccagt aattgttcac gcttgatcca
    15001 cacaacggga accccggtgc gagtcgcctg aacagtaaag gcatccggcc caaaacggtt
    15061 gcgcagttcg ccaattaccg gatcatcaag atgatcgcgg gtctgccagg cgggttcttg
    15121 cgcggttaag tcggtcatat tgttcaccat tgcaaatggt ccgtggtgac tgtcggcgca
    15181 gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa atccctttgc cgacaggcgc
    15241 aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca atacgttcac cgcgcttgcg
    15301 ttcgcgctct gattgcatat tggcgcgata aacgccctga tcgccaacca cccaggagag
    15361 cggacgacgt tctttgccga tagattcctg caacagcatc agtgcctgca tgtacgcttc
    15421 aggacgcggc gggcagcccg ggatatacac atcaaccggg atgaatttat cgacgccctg
    15481 cacaacggaa taaatatcgt acataccacc agagttggca caggcaccca ttgagataac
    15541 ccattttggt tccagcatct ggtcatacag acgctgaata accggtgcca ttttggtaaa
    15601 gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc gaagcacgca atacttctgc
    15661 gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc accatctcaa cgtaacagca
    15721 ggaaagaccg aagttatacg gccaaattga gtttttacga ccccagttaa ccatgtcatt
    15781 gagcttgccc ataaacacgt ttttgttaac ttcttgctcc agagggtcgg ttacgatctc
    15841 ctgcttttgc agggggtaac ggtcgttctc accgttggga tctatgcggg tgagcgtata
    15901 atccatctta atgcctcgcg gttagcgttg acgattagcg atactgttcg tttccgggtt
    15961 catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc gcgccaatac gcaccagata
    16021 aaccagacct gccagtaaca caaaaataaa aattgcagct tccacaaagc ctacccagcc
    16081 gctttcgcgg atagaggttg accatgcgaa cagatacagc gcttcaacgt cgaagataac
    16141 gaagaacatg gccaccagat aaaacttggc ggacaggcgt aagcgggcgg agccgaccga
    16201 gtcgataccg gattcaaacg gcacgttttt cgacctcgcg cgtgcgcgac cgcctaaaaa
    16261 ccaaccgcct accagcatca ggcaacacag gccaatggca acgataagaa agatagcgaa
    16321 tgcccagtga tgagcgatga cttcagtgga tgttgacata ctcattgctt actcatcaaa
    16381 agtagcgcca gattcactgc tcttcacggc agatggacgc cacatcgatt catggggagg
    16441 aataaaaaaa accttacaat cactgtagaa attcttttat acagctaatt gatgtggtct
    16501 tttactcctt tctataacct tttgtcaact ttaacaaaag tttcttcaca ttagtttaca
    16561 taatatcaac accattagca tttaatgccc tttcacccca gatccttgac gactccagga
    16621 taattagatg ttgttgaatc gtgtccgttg tgaagcaatg gaaaaaatac gggtctattt
    16681 tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt attttcttga tctgtgacac
    16741 gcttttgtca ttccataaca aaaacgcagc aacaaattta cgtatttttt aacatcattg
    16801 tagcaggtga tttttttcag gcgattattt gtgcgttcgg gacgtgaatc tctggtgatt
    16861 gaaaaatgaa cagttttgta cgttctgcac tatgcgagga aggattttta ctaaaaaaaa
    16921 gccgctgggg tttaaaacac ccccagcggc tcgtttttta cactattgtc tcaggaatta
    16981 tctatcgtcc gtcgattact cgatatccct ttcaatcaac aatgaatcat ccccttccgg
    17041 agcagacatt gggctgtatt gccacggatt atggtagctc tccatcgcct gatagatcac
    17101 ctgggccagc tcattattac tggacgggtc atagcacagc aaatattcgg tatcaggtag
    17161 cggcggtaag ccatctactc cactcagcac acgcaggtcc gggctcatca tctcaaccgg
    17221 ccttgccgtc acgccaagac cggctttcac tgccgcacga acggccggaa gcgtcgaggc
    17281 gacataagcc agtcgccatg gaatatctgc tttattaagc gtcgccagca ccatatcgcg
    17341 aaacggactt ggatcatcca gcaatacaag agggatcggc tcgccttttt gcaatacgta
    17401 ttccgctgcg cagtaccagt gtgttggcga ggttcgcagg ttcaatgcct taaacgctga
    17461 aggacgatgg gtggttacca tcaaatccac ttcctgggat tccagcattt ctgccatata
    17521 ggcattacgt ttaacgcgga catccagcgc taatttcgga taaaccgaac tcacgcgatt
    17581 taacaggaaa ggtaagatcg tatcggcaga ttcatctgaa gcaccgatag ttaacacgcc
    17641 ctgaagatta ctgaacatta atgatgagca ggcctcatca ttaaaacgca ggattttcct
    17701 ggcgtaacca agaagttgaa tgccatgttc agttaacagt ttgttgcgac cgtgacgagc
    17761 gaacagttct ttcccaacga gttgttccag acgctgcatt tgctgactta cggcggactg
    17821 agtacgacac acagcggcag ctgcggcagc aaaagtgttc agatcggcaa cagcaacaaa
    17881 tgttctcagc agatcgaggt cgaggttaat tatcggacga tttgcactta tcatatatta
    17941 tcacttactg gcggctcata ctgagctggt taatgctgtg cacacacaaa caagcaattc
    18001 catttgtaat gtgcctccct ggcagtttca tccggaaccc ggacgaaagt aaaaatgcat
    18061 atgagttgca ctaaaaaagc gactcacatt gttccgttat aatgcctgaa gtagatcaca
    18121 gaatatatct tcagggatcg catatctatt aagttactca ctcttttcta tttatgacat
    18181 gcgcgtgttt gtataaatgt aaatgtgagt ccttgttcca ctctcgtgca gcatcgctgg
    18241 tcatacgcga acacgtacaa acagcaatgg tgaggcggca tcaagagcag ggatccgtca
    18301 tttatgcgag cattttaccc caaaaacctt ttatttataa gggtcattgc gaattatctg
    18361 atgca
//
LOCUS       DAPGWD010000078        18359 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPGWD010000078 DAPGWD010000000
VERSION     DAPGWD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.078"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..148
                     /locus_tag="RHX88_002667"
     CDS             <1..148
                     /locus_tag="RHX88_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231338.1"
                     /translation="NGAPWEARTFINQLHCVSFRIKGFRGSGMRRVEDDHARQLQFVA
                     KMQE"
     gene            153..2393
                     /gene="fepA"
                     /locus_tag="RHX88_002668"
     CDS             153..2393
                     /gene="fepA"
                     /locus_tag="RHX88_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDV2231339.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            2568..3188
                     /gene="entD"
                     /locus_tag="RHX88_002669"
     CDS             2568..3188
                     /gene="entD"
                     /locus_tag="RHX88_002669"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDV2231340.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(3310..3462)
                     /gene="hokE"
                     /locus_tag="RHX88_002670"
     CDS             complement(3310..3462)
                     /gene="hokE"
                     /locus_tag="RHX88_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV2231341.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(3813..3965)
                     /gene="hokE"
                     /locus_tag="RHX88_002671"
     CDS             complement(3813..3965)
                     /gene="hokE"
                     /locus_tag="RHX88_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV2231342.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            4407..5525
                     /locus_tag="RHX88_002672"
     CDS             4407..5525
                     /locus_tag="RHX88_002672"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV2231343.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            5591..5839
                     /locus_tag="RHX88_002673"
     CDS             5591..5839
                     /locus_tag="RHX88_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDV2231344.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            5904..6272
                     /locus_tag="RHX88_002674"
     CDS             5904..6272
                     /locus_tag="RHX88_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV2231345.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            6366..7019
                     /gene="nfsB"
                     /locus_tag="RHX88_002675"
     CDS             6366..7019
                     /gene="nfsB"
                     /locus_tag="RHX88_002675"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDV2231346.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            7127..8374
                     /gene="ybdG"
                     /locus_tag="RHX88_002676"
     CDS             7127..8374
                     /gene="ybdG"
                     /locus_tag="RHX88_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDV2231347.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(8442..9818)
                     /gene="pheP"
                     /locus_tag="RHX88_002677"
     CDS             complement(8442..9818)
                     /gene="pheP"
                     /locus_tag="RHX88_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDV2231348.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(9920..13063)
                     /gene="cusA"
                     /locus_tag="RHX88_002678"
     CDS             complement(9920..13063)
                     /gene="cusA"
                     /locus_tag="RHX88_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDV2231349.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYSISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(13075..14298)
                     /gene="cusB"
                     /locus_tag="RHX88_002679"
     CDS             complement(13075..14298)
                     /gene="cusB"
                     /locus_tag="RHX88_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDV2231350.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQTEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(14314..14646)
                     /gene="cusF"
                     /locus_tag="RHX88_002680"
     CDS             complement(14314..14646)
                     /gene="cusF"
                     /locus_tag="RHX88_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDV2231351.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(14670..16052)
                     /gene="cusC"
                     /locus_tag="RHX88_002681"
     CDS             complement(14670..16052)
                     /gene="cusC"
                     /locus_tag="RHX88_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDV2231352.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            16209..16892
                     /gene="cusR"
                     /locus_tag="RHX88_002682"
     CDS             16209..16892
                     /gene="cusR"
                     /locus_tag="RHX88_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDV2231353.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            16882..18330
                     /gene="cusS"
                     /locus_tag="RHX88_002683"
     CDS             16882..18330
                     /gene="cusS"
                     /locus_tag="RHX88_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDV2231354.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         4454 a         4925 c         4651 g         4329 t
ORIGIN      
        1 taatggcgcg ccgtgggaag cgcggacatt tattaaccaa ctgcactgcg tgtctttcag
       61 gatcaaaggt tttcgcggta gcgggatgcg tcgtgttgag gacgaccatg cccgacagtt
      121 gcaattcgtg gcaaaaatgc aggaataaaa caatgaacaa gaagattcat tccctggcct
      181 tgttggtcaa tctggggatt tatggggtag cgcaggcaca agagccgacc gatactcctg
      241 tttcacatga cgataccatt gtcgttaccg ccgccgagca gaacttacag gcgcctggcg
      301 tttcgaccat caccgcagat gaaatccgca aaaaccccgt tgcccgcgat gtgtcggaga
      361 tcatccgtac catgccaggc gttaacctga ccggtaactc caccagtggt cagcgtggga
      421 ataaccgaca gattgatatt cgcggtatgg gtccggaaaa cacgctgatt ttgattgacg
      481 gcaagccggt aagcagccgt aactcggtgc gtcagggctg gcgtggcgag cgcgataccc
      541 gtggtgatac ctcctgggtg ccacctgaaa tgattgaacg tattgaagtt ctgcgtggtc
      601 cggcagctgc gcgttatggc aacggcgcgg cgggcggcgt ggttaacatc attaccaaaa
      661 aaggcagcgg tgagtggcac ggctcctggg acgcttattt caatgcacca gaacataaag
      721 aggaaggtgc cactaaacgc accaacttca gcctgaccgg tccgctgggc gacgaattca
      781 gcttccgctt gtatggcaac ctcgacaaaa cccaggctga cgcgtgggat atcaaccagg
      841 gccatcagtc cgcgcgtgcc ggaacgtatg ccacgacgtt accagccggg cgcgaagggg
      901 tgatcaataa agatattaac ggcgtggtgc gctgggactt cgcaccattg caatcgctgg
      961 aactggaagc gggttacagc cgccagggta acctgtatgc gggcgatacc cagaacacca
     1021 actctgacgc ttacactcgc tcgaaatatg gcgatgaaac caaccgcctg tatcgccaga
     1081 actacgcgct gacctggaac ggtggctggg ataacggcgt gaccaccagc aactgggtgc
     1141 agtatgaaca cacccgtaac tcgcgtattc cggaaggtct ggcgggcggt accgaaggga
     1201 aatttaacga aaaagcgaca caggatttcg tcgatatcga tctcgatgac gtgatgctgc
     1261 acagcgaagt taacctgccg attgatttcc tcgttaacca gacgctgacg ctgggtacgg
     1321 agtggaatca gcaacggatg aaggacttga gttccaatac ccaggcgctg accggggcga
     1381 acaccggcgg cgctattgat ggtgtgagtg ccaccgaccg tagcccgtat tcaaaagcag
     1441 aaattttctc gctgtttgcc gaaaacaaca tggagctgac tgacagcacc atcgtaacgc
     1501 cggggctgcg tttcgatcat cacagtattg tcggcaataa ctggagcccg gcgctgaaca
     1561 tatcgcaagg tttaggcgat gacttcacgc tcaaaatggg cattgcccgt gcttataaag
     1621 caccgagcct gtaccagact aacccgaact acattctcta cagtaaaggt cagggctgct
     1681 atgccagcgc gggcggctgc tatctgcaag gtaacgatga cctgaaagca gaaaccagca
     1741 tcaacaaaga gattggtctg gagttcaaac gcgacggctg gctggcgggc gtcacctggt
     1801 tccgtaacga ttatcgcaat aagattgaag cgggctatgt ggctgtaggg caaaacgcag
     1861 tcggcaccga tctctatcag tgggataacg tgccgaaagc agtggttgaa ggtctggaag
     1921 gatcgttaaa cgtaccagtt agcgaaacgg tgatgtggac caataacatc acttatatgc
     1981 tgaagagtga aaacaaaacc acgggcgacc gtttgtcgat catcccggag tacacgttga
     2041 actcaacgct gagctggcag gcgcgggaag atttgtcgat gcaaacgacc ttcacctggt
     2101 acggtaagca gcagccgaaa aaatacaact acaaaggtca gccagcggtt ggcccggaaa
     2161 ccaaagaaat cagtccttac agcattgtgg gtctgagcgc gacctgggat gtgacgaaga
     2221 atgtcagtct gaccggcggc gtggacaacc tgttcgacaa acgtttgtgg cgtgcgggta
     2281 atgcccagac cacgggcgat ctggcagggg ccaactatat cgcgggtgcc ggggcgtata
     2341 cctataacga gccgggacgt acgtggtaca tgagcgtaaa cactcacttc tgatgctaac
     2401 gtcagattgt tgacaaagtg cgcgtcgttc atgccggatg cggcgtgaac gccttatccg
     2461 gcctacaaaa atcttgccaa ttcaatatat tgcaggacca tgtaggcctg ataagcgtag
     2521 cgcatcaggc agtttagcgt ttgtcatcag tctctaatat ggtcgacatg aaaactacgc
     2581 atacctccct cccctttgcc ggacatacgc tgcattttgt tgagttcgat ccggcgaatt
     2641 tttgtgagca ggatttactc tggctgccgc attacgcgca actgcaacac gctggacgga
     2701 agcgcaaagc cgaacattta gccgggcgga tcgctgcaat ttatgcgctg cgggaatatg
     2761 gctataaatg tgtgcccgca atcggcgagc tacgccaacc ggtgtggcct gcggaggtat
     2821 acggcagtat tagccactgc ggggctacgg cattggccgt ggtttcccgt caaccgattg
     2881 gcgttgatat cgaagaaatt ttttctgcgc aaacagcgac cgaattgaca gacaacatta
     2941 ttacaccagc agaacacgag cgactcgcag actgcggttt agccttttct ctggcgctga
     3001 cactggcatt ttccgccaaa gagagcgcat ttaaggcaag tgagatccaa actgatgcag
     3061 gttttctgga ctatcagata attagctgga ataaacagca ggtcatcatt catcgtgaga
     3121 atgagatgtt tgctgtgcac tggcagataa aagaaaaaat agtcataact ctgtgccaac
     3181 acgattaatt gacaacatct ggtacgattc gcccgcagcc atcactgacc gcgggcgaaa
     3241 gtgtaaagca ggtgccttac cactctgacc tgacaaccgg atatgcgggg attgctcccc
     3301 gcacaacggc tacttcttcg gttcgtaagc gagaacagcc ttaaactcaa tattacgttc
     3361 ctttacggtg aactcacaca gcgagtctcc gaccagaagc gtaaatccca gtaccgtcaa
     3421 acacagcact atgactgcca caagggcata tttcgtcagc atgtttatat tgcctccatg
     3481 cgaagaaggg actaccatcc gagttgttga ggctcggagt ggcagcccca ggattgatag
     3541 aaatatctcc tggggcttca tctttccaga cctcagagtt aacctgaaac cggaaagctt
     3601 caggcacccg ccgttatctt acccttcctc aacaaaatag ctgtttattt atacagcgaa
     3661 tcctgtttcg gacttctgtc ggcaaaactc atcattgctc ttaaaacgaa atcgcccgca
     3721 gccatcactg accgcgggcg aaagtgtaaa gcaggtgcct taccatcctg acctgacaac
     3781 cggatatgcg gggattgctc cccgcacaac ggctacttct tcggttcgta agcgagaaca
     3841 accttaaact caatattacg ttcctttacg gtgaactcac acagcgaatc cccgaccaga
     3901 agcgtaaatc ccagcaccgt taaacacagc actatgactg ccgcaagggc atatttcgtc
     3961 agcatgttta tattgcctcc atgcgaagaa gggactacca tccaaattgt tcaggtttgg
     4021 gtaggtagcc ccagggttga tagaaatatc gtcctggggc ttcatctttc cagacctcag
     4081 agtaaacctg aaaccggaaa gcttcaggca cccgcagtta tcttaccctt gcttagcaaa
     4141 atagctgttt atttatacag caagtcctgt ttcggacttc tcactcccca tttgatcaac
     4201 tgaagctgaa cccgtttttt tctgcatgcc tcaatcgtca aaattcttcg ccaccagcca
     4261 gccgtgccat aacttgtcac attgcaaaag tagccaaatt gcttcagtca ccatcaccaa
     4321 aaaaagaaac attccagaac ttccgcatct cgcccagccg ctccgacttc tatactgaat
     4381 agaaaacgcc aacttaagag aaacctatgc cattacccga ttttcatgtt tctgaacctt
     4441 ttaccctcgg tattgaactg gaaatgcagg tggttaatcc gccgggctat gacttaagcc
     4501 aggactcttc aatgctgatt gacgcggtta aaaataagat cacggccgga gaggtaaagc
     4561 acgatatcac cgaaagtatg ctggagctgg cgacggatgt ttgccgtgat atcaaccagg
     4621 ctgccgggca gttttcagcg atgcagaaag tcgtattgca ggcagccgca gaccatcatc
     4681 tggaaatttg cggcggtggc acgcacccgt ttcagaaatg gcagcgtcag gaggtatgcg
     4741 acaacgaacg ctatcaacga acgctggaaa actttggcta tctcatccag caggcgaccg
     4801 tttttggtca gcatgtccat gttggctgcg ccagtggcga tgacgccatt tatttgctgc
     4861 acggcctgtc acggtttgtg ccgcacttca tcgccctttc cgccgcgtcg ccgtatatgc
     4921 agggaacgga tacgcgtttt gcctcctcac gaccgaatat tttttccgcc tttcccgata
     4981 atggcccgat gccgtgggtc agtaactggc aacaatttga agccctgttt cgctgcctga
     5041 gttacaccac gatgatcgac agcattaaag atctgcactg ggatattcgc cccagtcctc
     5101 attttggcac ggtggaggtt cgggtgatgg ataccccgtt aacccttagc cacgcggtaa
     5161 atatggcggg attaattcag gccaccgccc actggttact gacggaacgc ccgtttaaac
     5221 atcaggagaa agattacctg ctgtataaat tcaaccgttt ccaggcctgc cgctatgggc
     5281 tggaaggcgt cattaccgat ccgcacactg gcgatcgtcg accgctaacg gaagatacct
     5341 tgcgattgct ggaaaaaatc gccccttccg cacataaaat gggtgcatcg agcgcgattg
     5401 aggccctgca tcgccaggtc gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg
     5461 tcgccgatgg tggctcgctg attgggctgg tgaaaaagca ttgtgagatc tgggccggtg
     5521 actaagtagg gaattgcctt acagaagaat atccctgaca atggtgtttt aacttacatt
     5581 taacaatgtt atgaaacacc ctttagaaac cttgactacc gcagcaggca ttttgctgat
     5641 ggctttcctc tcttgcctgc tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa
     5701 actggtgacc atgttccatc tgatggatct tagccagctt tacactttat tgttttgtct
     5761 gtggttttta gtgctgggcg ctattgagta ttttgttctg cgctttatct ggcgacgctg
     5821 gttctcgctg gcggattaaa cgtcgattat cgccgtactt cgcggcatac tttgcttatt
     5881 ctccttcgcc taaaggaacg tttatggata agcaatcact gcacgaaacg gcgaaacgcc
     5941 tggcccttga gttacccttt gtcgagcttt gctggccttt tggcccggag ttcgatgttt
     6001 ttaaaattgg cggcaaaatt tttatgctgt cgtcggagct gcgcggcgtc ccctttatca
     6061 atctgaagtc cgatccacaa aaatccctgt taaatcagca gatatacccg agcattaagc
     6121 cagggtatca catgaataaa aagcactgga tttcggtgta tcccggcgag gaaatctccg
     6181 aagcgttact tcgcgatctg atcaacgatt cgtggaatct ggtggttgat ggtctggcta
     6241 aacgcgatca aaaaagagtg cgtccaggct aaagcggaaa tctatagcgc atttttctcg
     6301 cttaccattt ctcgttgaac cttgtaatct gctggcacgc aaaattactt tcacatggag
     6361 tctttatgga tatcatttct gtcgccttaa agcgtcattc cactaaggca tttgatgcca
     6421 gtaaaaaact taccccggaa caggccgagc agatcaaaac tctcctgcaa tacagcccat
     6481 ccagcaccaa ctcccagccg tggcatttta ttgttgccag cacggaagaa ggtaaagcgc
     6541 gtgttgccaa atccgctgcc ggtaattatg tgttcaacga acgtaaaata cttgatgcct
     6601 cgcacgtcgt ggtgttctgt gcaaaaaccg cgatggacga tgcctggctg aagctggttg
     6661 ttgaccagga agatgctgat ggccgctttg ccacgccgga agcgaaagcc gcgaacgata
     6721 aaggtcgcaa gttcttcgct gatatgcacc gtaaagatct gcatgatgat gcagagtgga
     6781 tggcaaaaca ggtttatctc aacgtcggta acttcctgct cggcgtggcg gcgctgggtc
     6841 tggacgcggt acccatcgaa ggttttgacg ccgccatcct cgatgcagaa tttggtctga
     6901 aagagaaagg ctacaccagt ctggtggttg ttccggtagg tcatcacagc gttgaagatt
     6961 ttaacgctac gctgccgaaa tcacgtctgc cgcaaaacat caccttaacc gaagtgtaat
     7021 tctctcttgc cgggcatctg cccggctatt tcccctcaga ttctcctgat ttgcataacc
     7081 ctgtttcagc cgtcatcata ggctgctgtt gtataaagga gacgttatgc aggatttaat
     7141 atcccaggtt gaagatttag cgggtattga gatcgatcac accacctcga tggtgatgat
     7201 tttcggtatt atttttctga ccgccgtcgt ggtgcatatt attttgcatt gggtggtact
     7261 gcggaccttc gaaaaacgtg ccatcgccag ttcacggctt tggttgcaaa tcattaccca
     7321 gaataaactc ttccaccgtt tagcttttac cctgcagggg attatcgtca atattcaggc
     7381 ggtattctgg ctgcaaaaag gcaccgaagc ggcagatatt ctgactacct gcgcgcagtt
     7441 gtggatcatg atgtatgcgc tgctttcagt cttctcgttg ctggatgtga ttttgaatct
     7501 ggcgcagaaa ttcccggcgg catctcagtt accgctgaaa gggatatttc aggggattaa
     7561 actgatcggc gcgattctgg tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc
     7621 agcgattctg atcagcggtc ttggtgcaat ggctgccgtg ctgatgttgg tttttaaaga
     7681 tccgattctt ggtctggtgg caggtattca gctttccgcg aacgatatgc tgaaactggg
     7741 cgactggctg gagatgccga aatacggcgc ggatggcgcg gtgatcgata ttgggttaac
     7801 caccgtcaaa gtgcgtaact gggacaatac cattaccact attcccacct ggtctctggt
     7861 ttctgactcc tttaaaaact ggagcgggat gtcagcatct ggcgggcgac gtattaagcg
     7921 cagtatcagt attgatgtca ccagtattcg ttttcttgat gaagacgaaa tgcaacgtct
     7981 gaataaagcg catttgttaa agccttattt aaccagccgc catcaggaaa ttaatgagtg
     8041 gaatcgccag caaggttcta cggagtcgat attaaacctg cgccgaatga ccaatattgg
     8101 aacctttcgt gcctatctga acgaatatct gcgtaaccat ccgcggattc gtaaagatat
     8161 gactttaatg gtacgccaac tggctccagg tgataacggt ttaccgctcg agatctatgc
     8221 gtttaccaac accgtggtgt ggctggaata tgaaagcatt caggctgata tattcgatca
     8281 catatttgcg attgtcgaag agtttggtct gcgacttcat cagtcgccaa ccggcaatga
     8341 tattcgctct ctggcgggtg catttaagca gtaattaaaa aaaccgctct catcgaatgg
     8401 atgagagcgg acatcgtttt gtcggatgta gcgtgaatgc cttatttccg acgcagcgtt
     8461 ttaaatgcca caaacaggaa tacaatccac accggcagca ggatcgctga caagcgcata
     8521 tcatccatcg tgcacatcag cagcaaaatc atgccgagga aggcaatgca aagatagttg
     8581 ccgaacggat agagcagcgc cttaaactgt gtttcacgcc cctgacgtcg catcgctgca
     8641 cgaaaacgca gatgcgccag gcagatcata atccagttca acagcagcgt tgctaccacc
     8701 agcgccatca gcagaccaaa cgctttttgc ggcagcagat agttgattaa taccaccagc
     8761 gaagtgatcg ctccggaaag catcagcgaa ttaatcggca caccgcgacg gctgacgcga
     8821 gtcaaaaact tcggcgcatt accctgcaca gaaaggccaa acagcatgcg gctgttagag
     8881 taaaccccgc tgttatacac tgacagcgat gctaccagaa tgacgaagtt cagcgcagaa
     8941 gctaccacgt tgctgtcgag attatggaaa atcatcacaa acgggctact gttggatttc
     9001 acttccaccc acggatagag cgccagtaaa accaccagtg agccgatgta aaacagcagg
     9061 atgcgataca ccacctgatt taccgctttt ggaatgcttt tttccggatc gcgcgcttca
     9121 gcggcagtaa tcccaatcag ctccagaccg ccgaaggaga acataattac cgccagcgac
     9181 aaaatcagcc cattccagcc ggtggcaaag aaaccaccgt agcgccagag gttgtcgata
     9241 ctggctttct cgccgccgtg accagaaaac agcagccaca ggccaaagcc gatcataccg
     9301 atgatcgcca gcactttaat cagcgcaaac cagaactcgg tttcaccata taagcgcacg
     9361 ttcaccaggt taacggcgtt gatgataata aagaaggcgg cagcccaaat ccacgttgga
     9421 acatccggga accagtactg catatagatg cccgcagcgg tcagctctgc cattcccacc
     9481 agcacgaaca ttacccagta gttccagcca gagaggaagc ccgcaaacgg tccccagtat
     9541 ttataggcaa agtgggcaaa tgaaccggat accggctcct caaccaccat ttcgccaagc
     9601 tggcgcataa tcaggaaagc gatgatcccg gcgacgccgt agcccagcaa tacagccgga
     9661 cccgccatct gaatcgccgg gccaatgcca agaaacagac cagtaccaat tgcgccaccc
     9721 aacgcaatca gttgaatatg acggttatgt aatccgcgat gaagcgtcgg ctcttgattc
     9781 gacgcagtat cttccgatac ggttgacgcg tttttcacgc ctttcccctg tgtgtctttt
     9841 ttgttgaggg gcacctttta acatttagtg cccatcgtcg caagacacaa tccacacggt
     9901 taaacggggt atcctgcttt tatttccgta cccgatgtcg gtgcagccac atcagcttat
     9961 acgccgccgg gataataaac agcgacagca aaggtgcggt gatcatgccg ccaatcatcg
    10021 gcgcggcaat ccggctcatc acctctgaac cagcccccgt tccccacaga atcggcagca
    10081 gaccggcgat aatcaccgcc accgtcatcg ctttcgggcg cacgcgcagg accgcgccgt
    10141 gatataacgc ctcatccagc ttctgctcgc tgaatgtttg tggattattc aacgacggca
    10201 cggcctctat ggcgtgacgt aaatacatca gcatcaccac gccaaattcg gcggcgaccc
    10261 cggcgagggc gataaagcca gtgcccgtcg ccacggaaag atgaaagccc atccaccaca
    10321 gcaaccagat gccgcccacc agcgcaaacg gtacgctgct gataatcagc aacgcttcgc
    10381 ccacccgacg gaacgccaga tacaacagca cgaagataat catcaacgtc atcggcacca
    10441 tgagtttaag cttatggttg gcgcgctcca gtagctcgaa ctgcccggag aatgccacgc
    10501 tggtgcccgg ttttaactgc actttttcag ctatcgcttt ttgcaaatcg tgaaccaccg
    10561 acaccatgtc acgatcgcgg gcatcgatat aaatccagct cgtcgggcgc gcattctcgg
    10621 ttttcagcat cgacggtccg gtagagactt taatgtcggc cacgtctgcc agggtgattt
    10681 gctgcttcat cggcgtcagg atcggcagct ggcgcagtgc ctgcggacta tcgcgccagc
    10741 tttgcggata acgcagatta atgggataac gggcaatccc ttccaccgtt tcgccaacca
    10801 tcgccccgcc caccgcagaa gtcacaaaca actgcacatc cgccaccgtc ataccgtaac
    10861 gtgcggcttt ttcacggtta atctcaacgt tgatatagcg cccaccttcc agccgctcgg
    10921 caagcgcaga agctacgcct ggcaccgttc gcgctacttc ttcaatttgc tcagccatcg
    10981 cgtcgatatc cgccagcaca gtgccggaaa ctttaatgcc gatggggctt ttaatgccgg
    11041 ttgagagcat atcgatacgg ttacgaattg gcggcaccca cagattcgcc agccccggca
    11101 gccgcacggt gttatccagt tcctcaatga ttttgtccat cgtcatgcct ggccgccact
    11161 gctcctgcgg cttaagctgg atggtcgttt ctaccatctc cagcggagca gaatcggtgg
    11221 cggtttccgc tttcccggtt ttgccaaata cccgcgccac ttcaggtacg ctcataatta
    11281 gcttgtcggt tttttgcagc atactcgccg cctctgctgc ggaaatcccc ggcagcgtcg
    11341 atggcatata caacaagtcg ccttcattga tctgcggtaa aaattccccg ccaactttat
    11401 tgagcggcca gagaaccgtc agcaccgaaa gcgccgccac cagcagcgtg gttttcggcc
    11461 agtgcagtac tttcagcaac agcggatgat aaacacgaat caaaaagcga ttgagcgggt
    11521 tactgctttc cggcggaatt ttgccacgga tccagtagcc catcaggatc gggatcacta
    11581 cgatcgccag cagcgccgca cccgccatcg catacgtttt ggtgaacgcc aacgggccaa
    11641 acagacgccc ttcctgccct tccagggtga agatcgggat aaacgacaac gtgataatca
    11701 gcagactgat aaatagcgcc ggccccactt caacagacgc atcggtgatc acctgccagc
    11761 gcgttttatt atccagcgtg gcgtcaggat gctggtgctg ccactcttcc agccgtttat
    11821 gtgcattttc gatcatgacg atagcggcat cgaccatcgc cccgacggca atcgcaatgc
    11881 cgcccaacga cataatattg gcattcagtc cctggaagtg catgacaata aaagcaatac
    11941 acaaccccag cggcaacgaa ataatcgcca ccagcgccga gcgcacatgc cagagaaaca
    12001 gcgcacagac taccgccacc acaataaact cttccagcaa cttgccgctg aggttgtcga
    12061 tggcgcggtc aatgagctgg ctgcgatcgt atgttgtaac tatctccacg ccttccggca
    12121 gactactttt cagcgtttcc agtttgtcct tcacggcggc gatcacttct cgggcgtttt
    12181 tgccggatcg caggatcacc accccgcccg ccacttcacc ttcgccattc agttcggcaa
    12241 tgccccggcg catctccggg ccaatctgga ccttcgcaac atcacgcaga taaacgggca
    12301 cgccattttc actggctttt aaaacgatgt gattaaagtc gtcgagcgtt tgcagatagc
    12361 cgctggcgcg caccatatat tccgcttccg ccagttcgat cgacgaaccg cccgcttcct
    12421 ggtttgaagc atccagcgcg ctttttactt cggctagact gatgctatat tgcgccaggc
    12481 gctggggatc gataaccacc tgatactctt tcaccacgcc gcccaccgac gccacttccg
    12541 caacgtcagg gatggttttt agctcatatt tgagaaacca gtcctgcaat gagcgtaaat
    12601 cggccagatc gtgcttaccg ctgcgatcca ccagtgcata ttcatagatc cagccaacac
    12661 ccgtggcatc tggtcccagc tcggcgctga ctcccgcagg cagcttaccc tgtacctggt
    12721 tgaggtactc cagcacccgc gaacgcgccc agtacggatc ggtgccatct tcgaaaatga
    12781 catacacata agagtcgcca aactgtgaga aaccgcgcac agtcttcgcg ccaggcaccg
    12841 acaacatggt ggtggttagc ggataagtca cctgattttc aacgatttgt ggtgcctgac
    12901 cgggatagct ggttttaata ataacctgca catcggagag atccggcagc gcatccactg
    12961 gcgtattaat gatggtccag gtgccccaga tgctcagaaa caacgcgccc atcagcacca
    13021 aaaaacggtt cgccaccgag cgacgaataa tccattcaat cattggttat tccctcaatg
    13081 cgcatgggta gcactttcag agcgcatccg ctccagtgcg ccagaaatat tggcttcgga
    13141 atcaatcagg aacaggccgc tggaaaccac cttttcacct tccgccagac cagagcgtaa
    13201 tgcggtgacg ccttgcgatg cctggaaaac agcaacgcgt ttcggtacaa agcgcccgtc
    13261 ggcatcaacg gtaatcaccc gctgttcgct gccggtatca atcagcgctt gtgacggaat
    13321 gagcagcatc ggttcgctgg cggtgttgag ttgcaaccag gcgttcatgc ccggttttag
    13381 cgcctcgtcg gcgttgtcga cttccagacg cagctgcagc gtgcgggtcg cggcatccac
    13441 gccaggtagc agcgtccatt tgcggatggt gagtgtttta tccggtcgcg ccggaacggt
    13501 gagggtaaac tgcgaggcat ctttcaccag ccaggcgatg gactccggga tcgcagcagt
    13561 gacccacacc gggtccatac cctgaatttt cgcgaccacg ttatctttgg cgatattcat
    13621 tcccgcgcgc agatcaaacg cggtgatcac gccatcaatg ggcgctttga gcgtaaagcg
    13681 agtctggatt ttttgcgtgg cgatcaggcg gcgaatatcc gcctccggca ttcccgccag
    13741 tcgcagccgc tcaagaatgc cttcagtctg ggtcgccgta ccgccggttt cgcgcagcag
    13801 taaatactca gtctgcgctt ccacccagtc aggaatggtc aggtcgagaa gcggtgtgcc
    13861 cttttgtact ttatcgccca cggtaagcgg atacaccttg tcgataaacc cggcagcgcg
    13921 agcctgcaca atggcatact gatactcgtt gtaactgaca ttcgccggga aactctgggc
    13981 aaaagtcagc ggtccgcgcg tgacggtagc cgttttcacc cccagattct gcgtctgagt
    14041 cgggtcaatg cgcacaccag acgcagaact ctcttcatcg gcatatttcg gcaccagatc
    14101 catatccata aacggcgatt tacctggttt atcgaaccgc gtattgggat acatcgggtc
    14161 gtaccagaat aagattttac gttctgcggt cgacgttttt tctgcaggcg gttccgcctt
    14221 tgcaacccag gtaaaacctg ccgcagaaat aataccgccc gcgatcatgc tgccgataat
    14281 aagcgcgatt tttttcatct cattaaacct gggttactgg ctgactttaa tatcctgtaa
    14341 taaagaaagg ttgccctgct ggacaaaatt aaacgccact ttgtcgccgg ttttaatttc
    14401 actcattttc gtctgcgggg tgatggtaaa gcgcatggtc atctccggcc agttcacagc
    14461 agcaatcgga tcgtgatgga tggtgatttt tttgctttcc agatcaatac cctttacctc
    14521 gccagtggca ctaataacct gtggttgtgc ttcgctcatg gtttcatgat gatgttcgtt
    14581 agcctgggca ttaaagccaa taacggtaaa cagactgaac attgcgactt gcagtgcttt
    14641 tttcatttat tctctcctgg agttaaaagt tattgctgcc aaccgccacc aagtgcggta
    14701 tataaagaaa tttcgttaac ctgacgggca taattcaggt caagtaaagt ttgtcgggtt
    14761 gcaaataacg aacgttcggc atccagcact tccagataac ttactgcgcc gtgctgatat
    14821 aatgcccgcg cccgttgcaa agtaatttgc agcgatgcca gataacgctg ctgggcgcta
    14881 atttgatcgt tcaggctttg acgtaatgcc agcgcatctg ccacttcttt aaaggcgttc
    14941 tggattttct gttcataatt cactaccgac tgctgctggc gaatttcggc gatatccaga
    15001 ttggcctggt tgcgtccggc attaaaaatg gggatctcaa ttttaggaat aaaattccac
    15061 atcccgctgc tggcgttaaa taatgacgac agatcgctgc tggcggttga tattccgctg
    15121 gtcaggctta tagatgggaa aaatgccgcg cgtgcggccc caatattggc attagccgcc
    15181 attaacgcgt gttcagcttc cataatatcc gggcgctgca ataagatttg cgacgacaag
    15241 cccgccggta atttaacgct ttgcaggctg tcgctgttta ctgtctgcgc ttgcggcagc
    15301 ttgccgtagc ttcccagcaa cagttgcaat gcattattcg cctgcgccag ttccccctga
    15361 cgtttagcga tgtcgctgcg ggtactttct atcaccccgc gagcctgttc cagcgccaga
    15421 acattactgc tacctgtcaa cagttgtttt tcgacaaacg catatgactg ctgataatta
    15481 cgcagcgttt cttcagctat ttgcaattgc gcatacgcca gttgctgatt gaaatagctt
    15541 tgcgcgacat tagaaaccag cagaatatgc accgcgcgct gagcttcctc agtggctaaa
    15601 taattttggc gctcggcttc gctcatgttc tttaagcgac cgaaaaaatc gaggtcaaag
    15661 ctggcgttaa ggccggtcga aaactcccgt gtcgtggcgg tattcccttt aagattaccg
    15721 ctccagctgc cgctgccctc gccattgagc tgtgggtagc ggtcggcatc ggtcagacga
    15781 tattgcgccc gtgcctcctg cactttcagc gtcgccatgc gcaaatcccg gttattcacc
    15841 agcgcctcgc taatcagcgt cttcacctga ttatcaacaa aaaaggtgcg ccagcccgcg
    15901 ttctgatagt tatctgctgc gttaacgagg ccgttctggc tgagtgagaa ctgctgcggt
    15961 acgggcattg ccgggcgctg ataatccggt gccagtgaac aaccggttag cgcaagggcc
    16021 acacaaaatg gcagaagttt acaaggagac ataggctcat aatttctgat gattttatgc
    16081 cgccaacttt actcgccagg ctctgatttt ccggtgacag gaaaatgaca aaattgtcat
    16141 tttgccaata agcgattgcc atctgatccc gctactctag aattgcccgg gcaacatgcg
    16201 gaggaaatat gaaactgttg attgtcgaag atgaaaagaa aaccggagaa tacttgacca
    16261 aagggttaac cgaagccggt tttgtggtcg atttggccga caacgggctg aatggctacc
    16321 atctggcgat gaccggtgat tatgatctga taatcctcga tattatgctg ccggacgtga
    16381 acggctggga tatcgtgcgc atgttacgct ccgccaataa agggatgccg attctgttgc
    16441 ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct ggagttgggg gcagatgact
    16501 acctgatgaa accattcgct tttgctgaac tgctggcgcg ggtgcgcact ctcctgcggc
    16561 gcggggcggc ggtaattatc gaaagtcagt ttcaggttgc cgacctgatg gtcgatctcg
    16621 tcagccgcaa agtcacccgc agcggcacgc gcatcacttt gaccagtaaa gagtttactc
    16681 tgctggagtt ttttctccgc catcagggcg aagtgctgcc ccgctcgctt atcgcctcgc
    16741 aggtgtggga catgaatttc gacagcgaca ctaacgccat tgatgtggcg gtgaagcggc
    16801 tacgcggcaa aatcgacaac gactttgagc cgaagctgat tcagaccgtg cgcggcgtgg
    16861 gctatatgct tgaggtgccg gatggtcagt aagccatttc agcgcccgtt ttcgctggca
    16921 acccgtctga ccttttttat cagcctggcc accatcgcgg cgtttttcgc ctttgcatgg
    16981 atcatgatcc actcagtgaa agtgcatttt gccgagcagg atattaatga tttaaaagaa
    17041 attagcgcca cccttgaacg ggtacttaat catcctgacg agacgcaagc ccgacgctta
    17101 atgacgctgg aagatatcgt cagtggttat tccaacgtgt tgatttccct ggcagatagc
    17161 cacggcaaaa cggtgtatca ctcccccggt gcgccggata tccgcgagtt tacgcgtgac
    17221 gccatacccg ataaagacgc gcggggcggt gaggtgtatc tcctttccgg cccgacgatg
    17281 atgatgccag gccacggtca cgggcatatg gaacacagca actggcggat gattaacttg
    17341 ccggttggcc cgttggtgga cggcaaaccg atttatacgc tctacatcgc gctttcgatc
    17401 gattttcatc ttcattacat aaatgatttg atgaataaac ttattatgac cgcatctgtc
    17461 atcagcatcc tgatcgtctt tatcgtgcta ttggcggtac ataaaggtca cgcgccgatc
    17521 cgcagcgtca gccgtcaaat ccagaatatc acctcgaaag atctcgacgt tcgccttgac
    17581 ccgcagaccg tgcccattga gctggaacag ctggtactgt cgttcaacca tatgatcgag
    17641 cgtattgagg atgtctttac ccgccagtcc aatttctcag cggatatcgc tcacgaaatt
    17701 cgcacgccga ttaccaatct catcacgcaa acggaaatcg ccctcagcca gtctcgcagc
    17761 cagaaggagc tggaagatgt gctctactct aatctcgaag agctgacgcg aatggcgaaa
    17821 atggtcagcg atatgctgtt tctcgctcag gccgataaca accagctaat ccccgaaaag
    17881 aaaatgctca acctggcgga tgaagtcggc aaagtgttcg attttttcga ggcgttagcg
    17941 gaagatcgcg gcgtggagtt gcggtttgtt ggcgacaaat gtcaggttgc gggcgatccg
    18001 ctgatgctgc gtcgggcgtt aagcaacctg ctttctaatg ccctgcgtta tacgccaccc
    18061 agtgaggcta ttgtagtgcg ctgccagacg gtcaatcatc aggtgcaagt ttccgtcgaa
    18121 aaccccggta cgcccattgc gcccgagcac ttaccgcgat tgtttgaccg tttctatcgc
    18181 gttgaccctt cccgccagcg aaaaggtgaa ggtagcggta ttgggctggc gatagtgaaa
    18241 tcgattgttg tcgcgcataa aggcacggtt gcggtaacgt cagatgcgcg ggggacaagg
    18301 tttgtgatta tgctgccgga gagagagtga ttattaagtt caaattcagg gcgtggtgc
//
LOCUS       DAPGWD010000079        18220 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPGWD010000079 DAPGWD010000000
VERSION     DAPGWD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.079"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(28..786)
                     /gene="aroD"
                     /locus_tag="RHX88_002684"
     CDS             complement(28..786)
                     /gene="aroD"
                     /locus_tag="RHX88_002684"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDV2231355.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(817..1683)
                     /gene="ydiB"
                     /locus_tag="RHX88_002685"
     CDS             complement(817..1683)
                     /gene="ydiB"
                     /locus_tag="RHX88_002685"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDV2231356.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1695..2960)
                     /locus_tag="RHX88_002686"
     CDS             complement(1695..2960)
                     /locus_tag="RHX88_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2231357.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3187..4401)
                     /locus_tag="RHX88_002687"
     CDS             complement(3187..4401)
                     /locus_tag="RHX88_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2231358.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMLINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4501..4857)
                     /locus_tag="RHX88_002688"
     CDS             complement(4501..4857)
                     /locus_tag="RHX88_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDV2231359.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4993..5100)
                     /gene="rprA"
                     /locus_tag="RHX88_002689"
     ncRNA           complement(4993..5100)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="RHX88_002689"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5286..6398)
                     /gene="ydiK"
                     /locus_tag="RHX88_002690"
     CDS             complement(5286..6398)
                     /gene="ydiK"
                     /locus_tag="RHX88_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDV2231360.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6787..9843
                     /locus_tag="RHX88_002691"
     CDS             6787..9843
                     /locus_tag="RHX88_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV2231361.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEEKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9840..10250
                     /gene="menI"
                     /locus_tag="RHX88_002692"
     CDS             9840..10250
                     /gene="menI"
                     /locus_tag="RHX88_002692"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDV2231362.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10350..10538
                     /locus_tag="RHX88_002693"
     CDS             10350..10538
                     /locus_tag="RHX88_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDV2231363.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11087..11455
                     /gene="sufA"
                     /locus_tag="RHX88_002694"
     CDS             11087..11455
                     /gene="sufA"
                     /locus_tag="RHX88_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDV2231364.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11464..12951
                     /gene="sufB"
                     /locus_tag="RHX88_002695"
     CDS             11464..12951
                     /gene="sufB"
                     /locus_tag="RHX88_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDV2231365.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12961..13707
                     /gene="sufC"
                     /locus_tag="RHX88_002696"
     CDS             12961..13707
                     /gene="sufC"
                     /locus_tag="RHX88_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDV2231366.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13682..14953
                     /gene="sufD"
                     /locus_tag="RHX88_002697"
     CDS             13682..14953
                     /gene="sufD"
                     /locus_tag="RHX88_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDV2231367.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14950..16170
                     /gene="sufS"
                     /locus_tag="RHX88_002698"
     CDS             14950..16170
                     /gene="sufS"
                     /locus_tag="RHX88_002698"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDV2231368.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16183..16599
                     /gene="sufE"
                     /locus_tag="RHX88_002699"
     CDS             16183..16599
                     /gene="sufE"
                     /locus_tag="RHX88_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDV2231369.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16748..17752
                     /gene="ldtE"
                     /locus_tag="RHX88_002700"
     CDS             16748..17752
                     /gene="ldtE"
                     /locus_tag="RHX88_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDV2231370.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17816..18052)
                     /gene="lpp"
                     /locus_tag="RHX88_002701"
     CDS             complement(17816..18052)
                     /gene="lpp"
                     /locus_tag="RHX88_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDV2231371.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4777 a         4404 c         4699 g         4340 t
ORIGIN      
        1 agggtgttcc cgccgaaata ttattgctta tgcctggtgt aaaatagtta ataccgtgcg
       61 taaatcattt accgagattt gccctggtgc agaggctttt tttaccgcac caaaagttgc
      121 cgccgagcca aatacttcac cagccagacg agaaattacg ccagtttttg ccatcgacat
      181 cgtgatgatt ggacgatcgg catactgctc ctgcatctcc agggtcgcgg taagcaacgt
      241 cagcacatcg ctggtacttt gcggcatcag cgcaatctta ggaatatcgg cgtcgaagga
      301 ctgcattttg cgcagacggg caatgatttc ttcggcttcc ggcgttttat ggaagtcatg
      361 gttggacatg acaactttca catcatgcgc gtgggcgtag gcgacggttt ctttaacctg
      421 atcatcgccg gtaaataact ccagatcgat catatcaacc aggccgctgt cgatggctgc
      481 acgattgaga gcgatataag cctcggtgga aatcgcctgc tcgccgcctt ctttggcact
      541 gcggaaggta aacagcagcg gtttttctgg catggtttca cggagaattt ttgccgccgc
      601 catgacagac tccacattgg agaggtcggc aaagtggtcc acacgccatt ccagaatatc
      661 aaagtccgct tcacgatagg cgagagcttc ggatttcacg cgggcgatat ctttcgccat
      721 cagcgagacg atgattttag gtgcgcccgt accaatgacg agatctttta cagttacggt
      781 tttcattttt taccctttct gcacgcggtc agcctgtcag gcaccgaacc ccatgacctg
      841 tttaacatat tccagaggga aatctttgcc agtccataac gtgaactgtt cagccccttg
      901 ccacaacaac atgccgtatc catcaatcgt tttgcaacca gcttgttgcg cctgctgcaa
      961 taacttcgtc atatgcgggt tatacacgca ttcagtgacc agcagtcccg gatgtaacag
     1021 actgatatca ttaactaatg attcattctc aaggggtttc atacccactt ttgtgccatt
     1081 ggttaaaatg tcggcggaag ccagggcttc agcaaaggct tgctgatcgg cgagatcggt
     1141 aaccgtgacg acacaatcgg tgttttcgtt aacccgctgc gcgaaggcga gggctttatc
     1201 gaagaactca tcccgacggt taaagagttt aatttctttt aaaccttcaa ttgccccctg
     1261 cgcgccaatc gccgttgagg cacctccggc ccctaacagc accattgttt tgcctttgat
     1321 atcaaaaccg ctctctttaa tggcgcgaat atggcccgtg ccgtcggtgt tatagccgcg
     1381 caggtagcca tcatcattaa cgatggtgtt gatggcaccc accagtttgg ctgctggcgt
     1441 taattcatca acatattcac acgccagttg tttgttcggc attgatatac cagttccgcg
     1501 cattttgagg gcttttaatc cttcaattgc tccgggaaaa ctatcgttat ccacttcaaa
     1561 ggccatatag gtaaatggca atcccgcttt ttctaaggct ttattttgca tttcaggcga
     1621 taaactgtgg cggataggat aggccatcag cccaatcaat tcgtattttg cggtaacatc
     1681 cataattaac tcctttaacc tctatgctta attgtgttta accgggtgct aaatttacgc
     1741 tcgccaaacc gcacatcatt ctcaggaata ataaaaacgc ggtaatagcg gataaaaaca
     1801 ataattgcgg taatcagggc cagcagcgcg aaagtaaaat cgagaacaat gatatattga
     1861 aggccgatgt tcgacagata accggtaatc agcggaataa caaagttagc cagtccaccc
     1921 atcatcatat aaatactggt gactttggct ttgctttttg ggaaaaactc tgacatgacc
     1981 gaaacaccga gctgtaaaat gccgccagct gctgagaaac cgataacaaa ggcgccggca
     2041 ttacacacca gtggagaagg gtacaggtag ataatggctg ctgttattgt tgccagtgca
     2101 gagttaaata cattagccca gatgggccgg accatttttt tcagtagtgc ggcaaaaata
     2161 aagacacaga ccaacgagcc catactgtaa taagagatgg tttttaatgc ctcagcttct
     2221 gacataccag caaaagccat cgcatatttg ggcatccaca ccacaatcac ataaaaggtc
     2281 gagaatgcgg ctacaccgaa cagtaccgat gaaacacctt ccagccagac taacggtttg
     2341 ctgttcattt gcggtaattc attagctacg ctggcgtcca ccaactggct ggggaatttg
     2401 cttttcaaca gcatcagcgt gatcagtaca aacagaatac ccggaataat cagcccatag
     2461 ccgtaccaga tattattgag caacatatag ctcaccagca ttgggtagaa catttgccca
     2521 aatgacacca tcgctttaac cagtatgacc gccgaaccag aggctttcgg aaagcattcc
     2581 atgagcgcgg ggtagccacc cgtatccagc gctgagttag cgatacctac gcacactgcc
     2641 agaccgtagg cgagagttaa attcgggcaa gcgggaatac caaagaagaa tagcagatac
     2701 attattactg ccattaatat caccgcccga cgaccaaact tatcggagat cacaccgaag
     2761 aataaaatac tgatcaatcg ccccaaacca ataccggaaa ttaagtaggc aatgcccgcg
     2821 ttgtcagtgg aaaacttttc cgccagagat gacatgtttt gggcaagcgt aataacacta
     2881 atgccgtgca ggaagtagct gaagtaaata caaagaacag ccaggataaa tggcgtgctg
     2941 aaagccttat tttgagacat tttcacagct ccttgctgaa agataatttc gttaccgtac
     3001 gtattgttct tcgacggttt acataattac tatactgaag atgattttcc ctgggtacat
     3061 ggcataaagt ttatgtttcc aggaatattg catgtcatag tttgtttttg atcgcgattt
     3121 tccttagtta gcgcagaaag agttatttat ccgtaagatc ttgcatgacc tgaaaagaat
     3181 attgcattac ccacccggag cgacattttc ctttagcgag tgatgccgcg tttttttgcg
     3241 gctgcgtaag ccgataaaca gtgccagtaa aaagccgatg gcagcgatgg cggtatcgaa
     3301 ccacataata tcggcaatac ttctttggga cagatgagcc gtaatcaacg gaatagtaaa
     3361 ggtcgcaata ctgcccgcac tgtaatagat ccctgtagct ttacctttag cgtaggggaa
     3421 acgttcagcc attaacgtca ggccaatttg cacaacacct ccagcagagg taaaaccaat
     3481 gacaaaagca aatattatca ccacataaaa tgtgggatgc aggcagacgg taaacagagc
     3541 aataaatgag ataaaggtgt acagcatcag taatgttgtt gggcgaacgg tatttcgaat
     3601 gagtggagcg gtaataaata cacaaagcag cgaacccacg gtgtagatac tgagtagttt
     3661 gatcgacata gtgtatgaca tgcctgcaac aaattgtccg tactgtgaca gccactggct
     3721 aaccagataa aacgttgcca ttgagatata gccatataag gtataactgg ctaaatcgat
     3781 aattgaacag cgatgttccg tagagcgggt ggttttcttt atgacaggta agcgacgacc
     3841 cggatggggt gggaacgtac aacggtataa aaacagagcg ttaataagca taatgcctgc
     3901 agcaatcata aaggaccaac cgaaccacag ttcagcccac actaacaggc taatgattag
     3961 cggtaataaa aattgtccgc tggaaacaaa tgctttaatt aaaatattgg ctgtcccagg
     4021 tgagcgtgga aaagcttcca tcaaactggg ataagtgcct gcatcgagaa aactgtttgc
     4081 cattcccgcc agaaagccaa aaacataagc gataatgatg ttattggtct gcaggatgcc
     4141 aaaaaagaag gccatatagc aacacatccc gagcatgata aaagggcggc gaccaaagcg
     4201 atcggataat aatcctgcaa aaagcaagac acttaatcga ccaatgccca gcgatgagat
     4261 aactatcgag acacccgcgg cattagtctg ccaaagtgtc tccagcgagg ccatattcag
     4321 gctcatcaaa atgacgccca taccatgcac caggtaatta aaatacaacc caagtgcggt
     4381 agggtaatag ggatttttca taggttctac ttagcaaagg tgctttaaaa agtgtagcga
     4441 tgttgtgtca gtatccattc attattgttg taacaacgac aattcctttc tgtggaaaaa
     4501 ttagcaaagc cgctccagat cgtgtgcata taattcagcg gctaccgatt gatagatctt
     4561 ccagtcaata aaattgccat cagggtagac tcgttgaaat gcggttaagt cggggaaaaa
     4621 acgcatggtg ttgttgccga tgcggttatt tattttctct atgatggcat gaagatgttt
     4681 ttttctttgc tgacgcatag ccaacagttg ctcgactaca cgatcaggaa tggcacactt
     4741 gccattttcc cattgacgcc agctttcact gtcaccggtc tgggcaatcc aggtcgcgca
     4801 ttcatcaata gtcatggcaa aaatatggcg gagtgcctgg agttcataag cgttcatcgt
     4861 gtaatggctg catgaaataa acaataaaat ataaataaca ttatcagcct gctgacggct
     4921 tgaagagagt cacagtatct tgtgcaacat tattggaaag agtgaggggc gaggtagcga
     4981 agcggaaaaa tgttaaaaaa aagcccatcg tgggagatgg gcaaagacta cacacagcaa
     5041 ttcgttgttt cactcagggg atttccatgc ttataaatca atatgttgat ttataaccgt
     5101 gagctaatag taggcatacg gactttttgc gtcgatcaga ttcgtctcat tagtttaaaa
     5161 actgtaaaga atttgcgaca ggtactacca gctgaatggc ggtaagcagg aaaatatggg
     5221 gaagagcaag caaatatcaa ttagcggtgg ttaacaatca ggcggcagag ccgcctgatg
     5281 agaaattact tattcggttt ctcgatttcg ccaagttctt cgagaatttc ttccggttgg
     5341 tccgtcggcg gcgggacttc ttccacccac gcggcaaaca gacgccagga aacggctaac
     5401 agaaccggac caataaacag accgatcatc ccgaaagcaa tcaaaccacc aataacgcca
     5461 gagaggatca ggatcagcgg taaatcggca cccatgcgaa ttaacattgg gcggatgacg
     5521 ttatccagcg tgccaaccac accgctccac actaacaata ccgttcccca ggtggtatcg
     5581 ccggtccagt agagccagat aatcgccgga atcagtaccg gcaacgggcc aagctggaca
     5641 aggcaggaga ggatcattaa caccgttagc aacgttgcat aaggtacgcc ggatacggcg
     5701 aggccgatac cgccaagcac tgcctgtact aacgccgtca ccaccacacc cagcgccacc
     5761 gcgcggatag cctgtgccgc cagcagcacg gcggcatctc cgcgaacgcc tgccagacgg
     5821 gttgcaaaat ggcgaatgcc ttgtgccacc tgttcaccgc gccagtacag cagggcactg
     5881 aagagaagca tcagcgcaca atgcaccata aagcgcccga tatgcgccgc ctgcccaacg
     5941 aaccaggtgg tggtggtgcc aatataaggg cggactttcg ccatgatcgc cgtccccccc
     6001 atatccagca agttgtgcca gcctgcatac agcttcgcgc caatcaccgg aatggtatta
     6061 agccacgcca gatcgggtaa cgtcatgtcg ccgctggaaa tggctttaat tagcgggccg
     6121 ctgccgtcga cgatactgtt aaccagcaaa gcaataggga tgataaacac catcactaat
     6181 aacagcgtca tcaccagaac ggcgagagag cggcggccaa acatgatctt ttgcaaacgt
     6241 aacaataccg gccaggtggc gataaccacc gtaccggccc atgcaaagcc gagaataaag
     6301 ggttgaacaa tccacagaca tgccacaatc atgatggcta aaaacagcac cgaaagcaga
     6361 atttgtgcga catccctggg ctgacgaaca tttaccataa ctacttttca ccttttttgt
     6421 gcgtcaaatc gtcgacgcga cgtgaacacg cgaaactcac ctgcataatg atgagcaatt
     6481 tcagcggttt ttaacaggcc gattctgccc ttaattctga tgggcgtgtt agaaaaaaat
     6541 gtggtacaac aactcaggca acacgcaaac gatttactcg tcgtatttca agttgcatgt
     6601 gctgcggcta cgttcgttca ccccagtcac ttactattgt aagctcctgg agttcactcg
     6661 cttgccgcct tcctgcaact cgaaatccat agagtaaata aacatcacct ttcagtgcgc
     6721 tattgcagcc gcctgaaaga atagtgtatc gacctacatc acagaccgca ggaaagggtc
     6781 aatataatga ttccacagat ttcccaggca cccggcgtcg ttcaactggt gcttaatttt
     6841 ttgcaagagc tggagcaaca aggttttacc ggcgatacgg cgacaagtta tgccgatcgt
     6901 ctgacaatgt cgaccgacaa cagtatttac caacttctcc ccgatgcggt ggtatttccg
     6961 cgttcaaccg cagatgtggc gctgatcgcc cgtcttgccg cgcaggaacg ctattcatcg
     7021 ctgatcttta ccccccgcgg cggcggcacc ggcactaacg gtcaggcgct caaccagggg
     7081 attattgttg atatgtcccg ccatatgaac cgcatcatcg aaattaaccc tgaagagggc
     7141 tgggtgcgcg ttgaggccgg ggtgataaaa gatcaactca atcagtacct gaaaccgttc
     7201 ggctactttt ttgcgccgga actttcgacc agcaaccggg caacgctcgg tgggatgatc
     7261 aataccgatg catccggtca gggatcgctg gtctatggca aaacgtcgga tcacgtactt
     7321 ggcgtacgcg cggtgttgtt ggggggcgat attctcgata cgcaaccttt acccgtcgaa
     7381 ctggcggaaa cgctgggtaa atccaatacc acaatcgggc gaatttataa cacggtttat
     7441 caacgttgcc gtcagcaacg ccagttaatc atcgacaact tccccaaact taaccgcttt
     7501 cttaccggtt acgatctgcg tcatgtcttt aacgatgaga tgaccgagtt cgacctgacg
     7561 cgcattctga cgggttcaga agggacgctg gcctttatta ccgaagcgcg gctggatatt
     7621 acgcccttgc ctaaagtgcg ccgtctggtg aacgtcaaat atgactcttt tgactccgcg
     7681 ctgcgtaacg cgccgtttat ggttgaggcg cgggcgcttt cggtagagac ggtggactca
     7741 aaagtgctga atctggcgcg ggaagatatt gtctggcatt ccgtcagcga gttgattacc
     7801 gatgtgcctg acaaagagat gctcgggctg aacattgtgg agtttgctgg tgatgatgag
     7861 gcgctgattg acgagcgagt taatgctctc tgtgtgcggc ttgatgagct gattgccagc
     7921 caacaggcag gtgtgattgg ctggcaggtg tgccgcgagc tggcgggtgt tgaacgtatc
     7981 tatgcgatgc gtaaaaaagc cgttggtctg cttggcaatg ccaaaggtgc cgctaagcca
     8041 attccgtttg ctgaggatac ctgcgtaccg ccggaacatc tggcggatta tattgctgaa
     8101 tttcgcgcgc tgctcgacag ccacggctta agctacggta tgttcggtca cgtcgacgca
     8161 ggtgttttgc acgtccgtcc agcgctggat atgtgcgatc ctcaacaaga gattttgatg
     8221 aagcaaatct ctgatgacgt ggtggcgctg actgcgaaat acggtggttt gttgtggggc
     8281 gagcacggca aaggttttcg cgctgaatac agcccggcgt ttttcggtga ggaacttttt
     8341 gcagaactgc gcaaagtgaa agcggcattt gacccgcata accgactcaa cccagggaag
     8401 atttgcccgc cagaaggtct cgatgcgccg atgatgaaag tggacgcggt gaagcgcggt
     8461 acattcgatc ggcagatccc cattgcggta cgccagcagt ggcgcggtgc gatggagtgt
     8521 aacggcaacg gtttatgctt caactttgat gcccgtagtc cgatgtgtcc gtcgatgaag
     8581 atcacccaga accggattca ttcaccgaaa gggcgcgcaa cgctggtgcg tgaatggctg
     8641 cgtttgttgg cggatcgcgg cgttgatcca ctcaaactgg aacaagaact gcctgaatcc
     8701 ggcgtcagtt tgcggacgtt aattgcccgc acgcgcaata gctggcatgc gaataaaggc
     8761 gaatatgact tctcacacga agtcaaagag gcgatgtcgg gctgtctggc ctgtaaagcg
     8821 tgttcgaccc agtgccccat caaaattgat gtgccggagt ttcgctctcg ttttctgcag
     8881 ctctatcaca cccgttattt acgcccgctg cgcgaccacc tcgtcgctac ggtcgagagc
     8941 tacgcgccgc tgatggcacg cgcgccgaaa acctttaact tcttcattaa ccagccgctg
     9001 gtgcgcaaac tctcggaaaa acatatcggc atggttgatt tgccactgct gtcggtcccc
     9061 tcgctacaac aacaaatggt ggggcatcgc tcggcaaaca tgacgctgga acagcttgaa
     9121 gctctcaatg cagagcagaa agcgcgcacg gtgttggtgg tgcaggaccc ctttaccagc
     9181 tattacgatg cgcaagtggt ggcggatttt gtccgtctgg ttgaaaaatt aggtttccag
     9241 ccggtgttac tgccattttc gccaaatggc aaagcccagc atattaaagg ttttcttaat
     9301 cgttttgcga agacggcgaa aaagacggcg gatttcctca accgtatggc gaagctgggg
     9361 atgccaatgg tgggcgtcga tccggcgctg gtactttgtt atcgcgatga atataaactg
     9421 gcgctgggcg aggaacgtgg cgagtttaac gtcttactgg cgaatgaatg gctggcaagc
     9481 gcacttgagt cacagccagt ggctacagtc agcggtgaat catggtattt ctttggtcac
     9541 tgtaccgagg ttaccgcctt gccgggcgcg ccagcacaat gggccgcgat atttgcccgt
     9601 tttggcgcga aactggaaaa tgtcagcgtg ggttgctgcg gcatggcagg gacttacgga
     9661 catgaagaga aaaaccataa aaattcgctc gggatctatg agttatcctg gcatcaggct
     9721 atgcagcgac tgccgcgtaa ccgctgtctg gcgaccggat attcctgccg tagccaggtc
     9781 aaacgggttg aaggcacggg ggtacgccat cctgtgcagg ctttactgga gattattaaa
     9841 tgatatggaa acgaaaaatc accctggaag cactgaatgc tatgggtgaa ggaaacatgg
     9901 tggggttgct ggatattcgc tttgaacata ttggtgatga cacccttgaa gcgacaatgc
     9961 cagtagactc gcggacaaag cagcctttcg ggttgctgca tggaggagca tccgtggtac
    10021 tggccgaaag tatcggttcc gttgccggtt atttatgtac tgaaggtgag caaaaagtgg
    10081 ttggcctgga aatcaatgct aaccacgtcc gctcggcacg agaagggcgg gtgcgcggcg
    10141 tatgcaaacc gttgcatctc ggttcgcgtc accaggtctg gcagattgaa atcttcgatg
    10201 agaaagggcg tttgtgctgt tcgtcacgat tgacgaccgc cattttgtga cgttgtcacc
    10261 agaaaagtgt gacgcatatt gcttttggtc attatctgca cagtaaacgg tggtatactg
    10321 tgcaggtctt gtctaaaagc gaggatgcta tgtctactca acttgatccc acccagctgg
    10381 ccattgaatt tttacgccgt gaccagagca atctttcacc tgcccaatat ctcaaacgtc
    10441 tgaaacaact agaacttgaa tttgctgacc tgcttactct ctcttctgct gaattaaaag
    10501 aagaaatcta ctttgcctgg cgtctgggcg tgcattgatc ctgacttaat gccgcaagtt
    10561 cgccaaatcg acctggatta acctccgtcc aggttcgatg accttcttaa tactgcttat
    10621 ctacccgcct gatattgttc aaaaataata ctaatcgcag tttgtgttaa aacggcgggt
    10681 tagctttatg agattattct tatcgcccct tcaagagcta agccactgag agtgccggag
    10741 ataagcgccg gatggggtag aaacccttaa gcctgtgtcg cacagactta agggttttct
    10801 tatttctgaa cgctgcactt ttttacctat ctcttaaata gtcattttca atacaacttt
    10861 ttctttttca tcacagcctt aatgctcctt agataataac cattatctaa caatgagata
    10921 cctaattctt agcgtgcctg ttaacccaca catcagggtc tatgcttatt aaaagcaggc
    10981 aaaaagcatg ttttacggta aagcccctgc gtttgctggg ttgaactgat aatcattatc
    11041 actaacatgc tgttatacgc tgaaagcgat gaagtgaggt aaatcgatgg acatgcattc
    11101 aggaaccttt aacccacaag atttcgcctg gcaaggctta acgctgacac ccgcagcggc
    11161 gatacacatc cgtgagctgg tggcaaagca gccgggtatg gtcggcgtgc gcttaggcgt
    11221 gaagcaaacg ggctgcgcgg gctttggcta tgtgctcgac agtgttagcg agccggacaa
    11281 agacgatctg ctgtttgaac acgacggcgc gaagctgttt gtcccgctgc aagcgatgcc
    11341 gtttattgat ggcacggaag tcgatttcgt tcgtgaagga cttaatcaga tattcaaatt
    11401 tcacaaccct aaagcccaga atgaatgtgg ctgtggcgaa agctttgggg tataggcggt
    11461 actatgtctc gtaatactga agcaactgac gatgtcaaaa catggaccgg cggcccgctg
    11521 aattataaag aaggattctt cacccagtta gccaccgatg agctggcaaa ggggataaac
    11581 gaagaggtgg tgcgcgcaat ttcggcgaag cgtaatgagc cggagtggat gctggagttt
    11641 cgtctaaacg cctatcgcgc atggctggag atggaagaac cgcactggtt gaaagcgcac
    11701 tacgacaagc tgaattatca ggattacagc tactactcag caccatcgtg cggtaattgt
    11761 gacgacactt gcgcgtctga acctggcgcg gtgcagcaaa ctggcgcgaa cgccttttta
    11821 agtaaagagg tggaggcggc gtttgagcag ttgggcgttc ccgtgcggga aggcaaagag
    11881 gtggcggtgg atgccatttt cgactctgtt tcggttgcca ctacttatcg cgaaaaactg
    11941 gcggagcagg gaattatttt ctgttccttt ggtgaggcga tccacgatca cccggaactg
    12001 gtgcgtaaat atctcggcac cgtggtgccg gggaatgaca acttctttgc cgcgcttaat
    12061 gcggcggtag cctctgacgg tacgtttatt tatgtgccta aaggcgtgcg ctgcccgatg
    12121 gaactttcca cctattttcg cattaacgcg gaaaaaactg ggcagtttga gcgcaccatt
    12181 ctggtggccg acgaagacag ctacgtcagc tacattgaag gctgttccgc tccggtgcgt
    12241 gacagctatc agttacacgc ggcagtggtg gaagtcatca tccataaaaa cgccgaggtg
    12301 aaatattcca cggtacaaaa ctggttccct ggcgataaca acaccggcgg tattctcaac
    12361 ttcgtcacca agcgtgcttt gtgcgaaggc gaaaacagca aaatgtcatg gacgcaatca
    12421 gaaaccgggt cagcgattac gtggaaatat cccagctgca ttttgcgcgg cgataactcc
    12481 attggtgagt tttactcagt ggcgctgacc agcggtcatc agcaagcgga taccggcacc
    12541 aagatgatcc acatcggtaa aaacaccaaa tcgaccatta tctcgaaagg gatctctgcc
    12601 ggacatagtc agaacagtta tcgcggctta gtgaaaatca tgccgacggc aaccaatgcg
    12661 cgcaatttca ctcagtgcga ctcaatgctg attggcgcta attgtggggc gcataccttc
    12721 ccgtatgtcg aatgtcgcaa taacagcgca caactggagc acgaggcaac gacatcacgt
    12781 attggtgaag atcaactgtt ttactgcctg caacgcggga tcagcgaaga agacgccatc
    12841 tcgatgattg ttaacggttt ctgcaaagac gtgttctcag aactgccgct ggaatttgcc
    12901 gttgaagcac aaaaactcct cgccatcagt cttgaacaca gcgtcggata aggaataaac
    12961 atgttaagta ttaaagattt acacgtcagc gtggaagata aagctatcct gcgtggatta
    13021 agcctcgacg ttcgtcccgg cgaagttcac gccattatgg ggccaaacgg ttcgggcaaa
    13081 agtaccttat cggcaacgct tgccgggcga gaagattatg aagtgacggg cggcacggtt
    13141 gagttcaaag gcaaagattt gcttgcgctg tcgccggaag atcgtgcagg cgaaggcatc
    13201 tttatggcct tccagtatcc ggtggagatt cccggcgtca gtaaccagtt tttcctgcaa
    13261 acggcactta atgcggtgcg cagctatcgc ggccaggaaa cgctcgaccg ctttgatttt
    13321 caggatttga tggaagagaa aatcgctctc ctgaagatgc cggaagattt attaacccgt
    13381 tcggtaaacg ttggtttttc cggcggcgag aaaaagcgca acgatatttt gcaaatggcg
    13441 gtgctggaac cggagttatg cattcttgat gagtcggact ccgggctgga tattgacgca
    13501 ttaaaagtgg tcgccgatgg cgtgaactcg ctgcgtgatg gcaagcgctc attcatcatt
    13561 gttacgcact accaacgcat tctcgactac atcaagcctg attacgttca tgtgctatat
    13621 cagggacgaa ttgtgaaatc cggcgatttc acgttggtca aacaactgga ggagcagggt
    13681 tatggctggc ttaccgaaca gcagtaatgc gctgcaacag tggcatcact tgtttgaagc
    13741 tgaagggaca aaacgctcac cgcaagcaca gcagcattta caacaattgc tgcgtaccgg
    13801 actgccgaca cgtaaacatg aaaactggaa atatacgccg ctggaagggc tgaccaatag
    13861 ccagtttgtc agcattgcgg gagagatatc cccacagcag cgagatgcct tagcgttaac
    13921 gttagacgct gtgcggctgg tatttgtcga tggacgttac gtgtcagcac tgagcgatgc
    13981 gactgaaggc agcgggtatg aagtgagcat taacgacgac cgtcaggggg tacccgacgc
    14041 tattcaggcg gaagtgtttc tgcatttgac ggaaagcctg gcacaaagcg tgacgcatat
    14101 cgccgtgaag cgcggtcaac ggccggcaaa gccattgctg ttaatgcata ttacccaggg
    14161 cgtggcaggt gaagaggtca acactgccca ttaccgacat catctggatc tggcggaagg
    14221 tgccgaagca acggtgatcg aacattttgt cagccttaat gatgctcgtc acttcaccgg
    14281 cgcacggttc actatcaatg tcgcagcgaa cgcccacttg cagcatatca agctggcgtt
    14341 tgaaaacccg gtcagtcacc actttgccca taacgatttg ttgctggctg acgatgccac
    14401 cgcatttagc catagtttcc tgctgggtgg cgcagtgtta cgacacaaca ccagtacgca
    14461 actcaatggc gaaaacagca cgctgcggat caatagcctg gcgatgccgg tgaaaaacga
    14521 ggtgtgtgat acccgtacct ggctggaaca caataaaggt ttttgtaaca gccgacagtt
    14581 gcacaaaact atcgtcagcg acaaaggccg cgcggtattt aacggtttga tcaacgtcgc
    14641 gcagcacgcc atcaaaacgg atggtcagat gaccaacaat aatctgctga tgggcaaact
    14701 ggcggaagtg gatacaaaac cgcagctgga aatctatgca gatgatgtga aatgcagcca
    14761 cggcgcgacg gtggggcgta ttgatgatga acagatgttc tatctgcgct cgcgcgggat
    14821 caatcagcag gatgcccagc agatgatcat ttacgccttt gctgccgaac tgacggaagc
    14881 actgcgtgat gaggggctta aacagcaggt gctggcccga atcggtcaac ggctgccagg
    14941 aggtgcaaga tgactttttc cgtcgacaaa gtgcgggccg actttccggt gctttcgcgt
    15001 gaggtaaacg gtttgccgct ggcttatctc gacagcgccg ccagtgcaca gaaaccgagc
    15061 caggtgattg acgccgaggc cgagttttat cgtcatggct acgcggcggt gcatcgcggt
    15121 attcatacct taagcgctca ggcgaccgag aaaatggaga acgtgcgcaa gcgggcatcg
    15181 ctgtttatta atgcccgttc ggcggaagag ctggtgttcg tccgcggcac gacggaaggg
    15241 atcaatctgg tcgccaatag ctggggcaac agcaatgtgc gggcgggcga taacatcatc
    15301 atcagtcaga tggagcacca cgctaacatt gtcccctggc agatgctttg cgcacgcgtt
    15361 ggcgcagagc tgcgtgtgat ccctctcaac cccgacggta cgctgcaact ggagacgctg
    15421 cctacgctgt ttgatgagaa aactcgcctg ctggcaatta ctcatgtctc caacgtgctg
    15481 ggaacagaaa atccgctggc ggaaataatc acgcttgcgc accagcatgg cgcaaaagtg
    15541 ctggtggatg gcgctcaggc ggtgatgcat catccggtgg atgttcaggc gctggattgc
    15601 gacttttacg tgttctccgg gcataaactg tatggcccca ccggaattgg cattctttat
    15661 gtgaaagaag ccttgttgca ggagatgccg ccgtgggaag ggggcggttc tatgatcgcc
    15721 accgtcagcc tgagtgaagg cactacctgg accaaagcac catggcggtt tgaagccggt
    15781 acacccaata ccgggggcat cattggtctt ggcgcggcgc tggagtatgt ttcggcgctg
    15841 gggcttaata gcatagccga gtatgaacag aatctgatgc attatgcgct atcacagctg
    15901 gaatctgtac cggatctcac tctctatggc ccacaaaaca ggcttggcgt tattgctttt
    15961 aatctcggta aacaccacgc ctatgatgtt ggcagttttc tcgataatta cggcattgct
    16021 gtgcgtaccg gacatcactg cgctatgcca ttaatggcct attacaacgt ccctgcgatg
    16081 tgtcgggcgt cgctggccat gtataacacc catgaagaag tggatcgtct ggtgaccgga
    16141 ctgcaacgta ttcaccgtct gctgggataa cagggaggca ctatggcttt attgccggat
    16201 aaagaaaaat tgctgcgtaa ttttttacgc tgcgccaatt gggaagagaa atatctctac
    16261 attattgagc tgggccagcg tctgccagaa ttacgcgacg aagaccgaag cccacaaaat
    16321 agcattcagg gctgccagag tcaggtgtgg attgtcatgc gccagaatgc gcagggaatt
    16381 attgaattgc agggcgacag cgatgcggcg attgtgaaag ggcttattgc ggtcgtattt
    16441 attctctacg atcagatgac gccgcaggat attgtcaatt tcgatgtgcg tccgtggttt
    16501 gaaaaaatgg cgctcaccca acatctcacc ccatctcgtt cacaaggtct ggaagcgatg
    16561 attcgcgcaa ttcgcgccaa agccgctgca cttagctaaa ctaaagggac agctttcatc
    16621 cggttggctc acattgccgg atggtcaatg tttacactga cctgcacagg agtgttttgg
    16681 tttcaggtga acataagcgc catccgacgt ttcagcgtga gtctccggca aatacaggag
    16741 gtttacaatg aaacgcgcgt ctttgcttac actcacgctt atcggcgctt ttagcgccat
    16801 ccaggctgcc tgggcggttg attatccgct accaccaacc ggaagccgac tggttgggca
    16861 aaatcaaacg tatacggtgc aagaagggga taaaaacctt caggctattg cccgacgttt
    16921 tgatactgcg gcaatgttga tcctcgaagc caataacact atcgccccgg tgccaaaacc
    16981 tggtacgacg ataactattc cttcacaact gttattacct gatgcaccgc gtcaggggat
    17041 tatcgttaac cttgcagagc tgcgccttta ttattatccg ccgggagaaa atattgtgca
    17101 ggtttatcca ataggtattg gattgcaggg gctggaaacg ccggtgatgg aaacgcgtgt
    17161 tgggcagaaa atccctaacc caacctggac gcctacggca ggcattcgtc aacgttcgct
    17221 ggagcgtggc attaaattac cgccagtcgt tcctgccgga ccaaataacc cgctaggacg
    17281 ttacgcactg cgcctcgcgc atggtaatgg cgaatacctc attcatggta ccagtgcgcc
    17341 ggacagcgtc ggtttgcgcg tcagttcagg gtgtattcgc atgaatgcac cggatattaa
    17401 agccttgttc tccagcgtgc ggacgggaac gccggtgaaa gtgatcaacg aaccggtgaa
    17461 atattccgtg gaacctaacg ggatgcgtta tgttgaagta catcgaccac tatcagcaga
    17521 agaacagcaa aacgttcaga caatgccata cacactgcca gcaggcttta cgcaatttaa
    17581 agacaataag gctgtagatc agaagttagt cgataaagcg ttgtatcgtc gggcagggta
    17641 tccggtttcg gtgagcagtg gagcaactcc cgcagccagc aatgcgcctt cagtagagtc
    17701 agcgcagaat ggtgaaccag agcaagggaa tatgttacgc gcgacgcagt agcggtaaac
    17761 ggcagacaaa aaaaatggcg cacaatgtgc gccatttttc acttcacagg tactattact
    17821 tgcggtattt agtagccatg ttgtccagac gctggttagc acgagctgcg tcatctttag
    17881 cagcctgaac gtcggaacgc attgcgttca cgtcgttgct cagctggtca actttagcgt
    17941 tcagagtctg aacgtcagaa gacagctgat cgattttagc gttgctggag caacctgcca
    18001 gcagagtaga acccaggatt accgcgccca gtaccagttt agtagctttc attattaata
    18061 ccctctagat tgagttaatc tccatgtagc gttacaagta ttacacaaag ttttttatgt
    18121 tgagaatatt tttttgatgg gaaggcactt atttttgatc gttcgctcaa agaagcatcg
    18181 aacgctgggt tttagataaa aaaattgaga gaaaacagga
//
LOCUS       DAPGWD010000080        18123 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPGWD010000080 DAPGWD010000000
VERSION     DAPGWD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.080"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            29..568
                     /locus_tag="RHX88_002702"
     CDS             29..568
                     /locus_tag="RHX88_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDV2231372.1"
                     /translation="MELLMSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPV
                     AATIRGNPVYALQHVGPALFSVKHEAADSVHNPSRMEPKERKDWYQSENERIKLEKEQ
                     RKLIPVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQE
                     KSYEACAAELIPDEEGESL"
     gene            577..2676
                     /locus_tag="RHX88_002703"
     CDS             577..2676
                     /locus_tag="RHX88_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDV2231373.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTYGGWL"
     gene            2673..2885
                     /locus_tag="RHX88_002704"
     CDS             2673..2885
                     /locus_tag="RHX88_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231374.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            2885..4393
                     /locus_tag="RHX88_002705"
     CDS             2885..4393
                     /locus_tag="RHX88_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV2231375.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRNLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            4338..6365
                     /locus_tag="RHX88_002706"
     CDS             4338..6365
                     /locus_tag="RHX88_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDV2231376.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDRVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFD
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTLDHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            6452..6775
                     /locus_tag="RHX88_002707"
     CDS             6452..6775
                     /locus_tag="RHX88_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDV2231377.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGLTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            6768..7043
                     /locus_tag="RHX88_002708"
     CDS             6768..7043
                     /locus_tag="RHX88_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HDV2231378.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            7055..7633
                     /locus_tag="RHX88_002709"
     CDS             7055..7633
                     /locus_tag="RHX88_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231379.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPLGKMTARIRVNRGNLPAIKLGTARVRLTRR
                     GGKLQYRGIVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            7630..8031
                     /locus_tag="RHX88_002710"
     CDS             7630..8031
                     /locus_tag="RHX88_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231380.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8042..8785
                     /locus_tag="RHX88_002711"
     CDS             8042..8785
                     /locus_tag="RHX88_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231381.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKRLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            8846..9232
                     /locus_tag="RHX88_002712"
     CDS             8846..9232
                     /locus_tag="RHX88_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDV2231382.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFLQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            9241..9570
                     /locus_tag="RHX88_002713"
     CDS             9241..9570
                     /locus_tag="RHX88_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDV2231383.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            9542..12607
                     /locus_tag="RHX88_002714"
     CDS             9542..12607
                     /locus_tag="RHX88_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV2231384.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHELMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAVASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12607..12936
                     /locus_tag="RHX88_002715"
     CDS             12607..12936
                     /locus_tag="RHX88_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231385.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            12946..13644
                     /locus_tag="RHX88_002716"
     CDS             12946..13644
                     /locus_tag="RHX88_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV2231386.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTVVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            13650..14393
                     /locus_tag="RHX88_002717"
     CDS             13650..14393
                     /locus_tag="RHX88_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2231387.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNMEAT
                     GFYRVPLTEAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLVAVSTFV"
     gene            14291..14938
                     /locus_tag="RHX88_002718"
     CDS             14291..14938
                     /locus_tag="RHX88_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV2231388.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     VTQLPAFRQKLNEGWYQIRIAGRDTGENELSARLNEPLENGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            14999..>18123
                     /locus_tag="RHX88_002719"
     CDS             14999..>18123
                     /locus_tag="RHX88_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV2231389.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKST
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTQTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGK"
BASE COUNT         4336 a         4302 c         5573 g         3912 t
ORIGIN      
        1 ataaataaac atgattttca cctgccagat ggagttgctt atgtcaaatg tgagcgggat
       61 cggtgatgct tattactgga gtgtttttaa aatcgctgag gcctttgggc ttcaccggga
      121 cacagtaaaa aaacggctcc tcgcggccaa cactcctgtg gctgcgacta tcagggggaa
      181 ccccgtttac gccctgcagc atgtcgggcc agccctgttt agtgtgaagc atgaggcagc
      241 agactctgtt cataatccat cccgtatgga gccgaaagag agaaaggact ggtaccaatc
      301 tgaaaatgaa aggatcaagc tggaaaagga gcagcgaaaa ctcatccccg ttgatgaagt
      361 agtcatcgtc tattcgtcca tgagaaaggc tgtcgtccag gttctggaga caattccgga
      421 tgttcttgaa cgcgattgcg ccctgactcc tcaggccgtc ggcgttgtac agcaggccat
      481 tgatgacctg cgatacactc ttcaggaaaa atcctacgag gcttgtgctg ctgaattaat
      541 tcctgatgag gaaggagaga gtctctagga ggaataatgg gtttttcatc agcccgaaat
      601 ttgggaaggg acatatcggc aggattttcc ccaccacgtc gcatgccgat ttcggaggct
      661 gttaaaaaat tcatgcgtgt tcccaagggg gctggtaact cggtgccatg ggatcctgaa
      721 ctgacaccct acatcattga gcccatgaac tgcctggcat cgcgtgaata cgatgcggtg
      781 atttttgttg gtcctgcgcg aacagggaag accattggtc tgatcgatgg atggattgtc
      841 tataccatcg tttgcgatcc ttcggacatg ctcgttgtgc agatgaccga agataaggcc
      901 cgcgagcatt ctaaaaagcg cctcgacaga acgttcagaa gcagtgcggc ggtaaagaaa
      961 agaatgagtc cacgtcgtaa cgacaataat gtccatgata agacgttcag ggatggctcg
     1021 ttccttaaaa ttggttggcc ctcggtcaac attatgtcgt cgtcggatta ccggtttgtc
     1081 gccttaaccg attacgaccg ttttccggag aatatcgaca gcgagggtga tggtttctcc
     1141 ctggcctcaa aacgtaccac cacatttatg tccgccggga tgactctggt ggagagctcg
     1201 ccgggacgtg acatctgcga cagcaaatgg cgacgtaagt cgcctcatga agcgccaccg
     1261 acgactggta ttctttccct ttacaatcgt ggtgaccgcc gccgctggta ctggtcatgt
     1321 ccgcactgcg gtgaatattt tcagccagcc atggatgcca tgaccggcta ccgtaatgaa
     1381 ccggatccct ttaaagccag tgaggcggcg tatctacttt gcccgcactg cagcggcatt
     1441 atcactgcgg agaaaaagcg tgagctcaat agtgcaggag tctggttgcg tgaaggtcag
     1501 gtcattgatc gtaacggcaa cgtttccggt gaaccgcgcc gctcccgtat cgccagtttc
     1561 tggatggaag ggccagctgc tgcgtatcag acctgggcgc aactggttta caaattactg
     1621 actgcagaac aggagtatga agcgacagga agcgaagaaa cactcagggc ggttatcaac
     1681 accgactggg gattgcctta tcttcctcgt gccagcatgg agcaacgaaa aagtgaactg
     1741 cttgagcagc gggcagagcc agttccttcc cgcagtgtgc cggatggcgt taatttcctt
     1801 gtggcgacag tggatgtgca ggcgggacgt catcgccgtt ttgtggttca ggtaacgggc
     1861 tatggcagtc gtggcgaacg ctggattatt gatcgttaca acatcacgca gtcattgcgc
     1921 agtgactgcg acggggagag ccagcgaatt gatccggcca gctatccgga agactgggat
     1981 gtcctgctga cggatgtttt tcataaaagc tggccgctgg cctccgatcc ttctcaacaa
     2041 atgcgactga tggcaatggc ggtggactcc ggcggtgaag acggggtcac tgataatgcc
     2101 tataaattct ggcgtcgttg ccgtcgtgat ggccttggta aacgtattta cctgtttaag
     2161 ggtgacagca tccggcgcgc aaaactgatc agccgtacat tccctgataa caccggacga
     2221 acgggccgcc gggcgcaggc cgcaggtgat gtgccgctct ggcttcttca gacggatgcc
     2281 ctgaaagacc gggtgaataa cgcgttatgg cgtgactcgc caggtccagg ctatgtgcat
     2341 ttccctgact ggctggggag ctggttttac gacgaactga cgtatgaaga gcggagcagt
     2401 gacgggaaat ggagtaagcc gggtcgcggt gccaacgaag cttttgacct gatggtgtat
     2461 gccgaggctc tggtcattct gcatggatac gaaaagatcc gctggccgga tgcaccggag
     2521 tgggcgagcc gggagacctg gctggagtgt gtccaggaca gtaccgaacc gtcatcctca
     2581 ccggaaccgg tatccacgcc tgttaaaaaa caaaaacgga agaaaacagt aactgacgat
     2641 gttaacccct ggctgactta cggaggatgg ttatgaacca gaatgatatc gaagccatga
     2701 ttcagcgtta tacggaagct gaaatggcgg tgctggacgg aaaatccgtc acctttaatg
     2761 gtcagcagat gaccatggaa aacttatctg agatccggca gggacggcag gagtgggagc
     2821 gccgccttgc ggctctgatt acacgacgac gggggcatcc cgggtaccgg ctggcgaggt
     2881 tctgatggca attcttgatg atgtgattgg cgttttttca ccaggatgga aagcggcaag
     2941 gctgcgttcc cgtgcggtga tccaggctta tgaggccgta aaaacgacgc ggacacacaa
     3001 agcccgacgg gagaaccgaa ctgccgacca gttaagccag tacggggccg tgtcgttacg
     3061 tgagcaggcc cgttaccttg ataacaacca cgatctggtt attggtgtat ttgacaagct
     3121 ggaagaacgg gtggtgggga aaaacgggat tattgtcgag ccacatccgg tattacgcaa
     3181 tggggccatt gcccgtgacc tggctgcgga gattcgcacc cgatggagtg aatggtctgt
     3241 cagcccggaa gtcaccgggc agtttacccg tccgatgctg gaacgtctga tgcttcgtac
     3301 ctggctgcgc gatggtgagg tgtttgccca gatggtttcc gggcgcataa acagcctggc
     3361 gccttctgcc ggtgttcatt tctggctgga ggcgctcgag ccggacttta ttcccatgac
     3421 cagtgatgag agcaacaggc tgaatcaggg cgtgtttgtt gatgactggg ggcgtcccga
     3481 aaaatatctg gtgtataaaa gccgtcccgt atccggacgg cagatggaaa ccaaagaagt
     3541 ggatgcagag cgaatgctgc atcttaaatt tgttcgccgt ctgcaccaga tgcgcgggac
     3601 gtctttgttg tccggtgtgc tgatccgcct cagtgccctg aaagaatatg aagattctga
     3661 gctgactgca gcaaggatcg ccgctgctct ggggatgtac atccggaaag gcgacgggca
     3721 gagctatgaa ccggatggta atggcagaaa ggagaatgaa cgtgagctta ccattcagcc
     3781 gggcattatt tacgacgatc tgaaacccgg cgaagaaatc ggaatggtga agtcggatcg
     3841 ccccaatcct aaccttgaaa cttttcgtaa tggtcagttg cgtgccgtgg cggcgggcag
     3901 tcgtctgagt ttttccagta cagcgcgcaa ctataacggc acttacagcg cccagcgtca
     3961 ggagctggtt gaatccactg atggctacct gatcctgcag gactggttta ttggtgccgt
     4021 cacccgcccg atgtatcgtg catggctgaa acaggctgtg gcatccggtg ttatcaggct
     4081 accccgcgat cttgaccgtt cttcactgta taccgcggtg tattccggac cagtgatgcc
     4141 gtggattgac cctgttaagg aggctgaggc ctggaaaatc cagattcgtg gtggagcggc
     4201 gacagaatca gactgggtac gtgctggtgg tcgtaatccg gatgatgtca aacgtcggcg
     4261 caaggccgaa attgatgaaa accgcaatct ggatctggta tttgataccg atccggccag
     4321 tgataaagga ggcagtagtg ccgcaacgaa acgacaggag ccgcagcaca ccgacgacca
     4381 gtccgaagaa taattcctgg ttcaggatgc aggctggtca ccagagtgac gcggatattt
     4441 atatttatga cgagattggt ttctggggtg ttacagcgaa gcagtttatc agtgatctga
     4501 atgcactggg cgatatcacc cacattaatc tccatattaa ttcaccgggt ggcgatgtct
     4561 ttgaaggcat cgccattttt aatgcgctga aaacacatgg tgcgtccatt accgtttatg
     4621 tcgacggtgt ggcggcgtca atggcgtcgg tcattgcgat ggtaggaaac ccggtcatta
     4681 tgccggaaaa caccttcatg atgattcata aaccatttgg ctttacgggc ggtgatgcgg
     4741 aggacatgcg cacctatgcc gacctgctcg atagagttga ggcggttctg ttacccgctt
     4801 atgcacagaa aaccgggaaa accaccgatg aaattgctgc catgctggcg gatgagacct
     4861 ggatgtccgg agccgaatgt ctggctcatg gatttgctga tcaggtgacg ccagccgtta
     4921 aggcaatggc atgtattcag tcaaaacgta cagaggaatt taaaaagatg ccggaatcca
     4981 ttcgaaacat gattactccg ccacgcaaca gtgctccacg cgtacaggat gatgaacctg
     5041 cagcctcccg gacgccagtg caggcagcag cacccgtggt ggatgaaaac agcatccgtg
     5101 cgcaggtact ggcagagcaa aaagcgcgtg taaacggtat taatgatctg tttgccatgt
     5161 ttgacgggcg ttatcagacg ctgcaggctc agtgtcttgc cgatcctgaa tgttcgctgg
     5221 agcaggcccg cgaaaagctg ttgaacgaga tgggtcgcga gtccacgcca tccaataaaa
     5281 ataccctgga tcatatttat gccggtaacg gtaattttgt gggggacggg atccgccagg
     5341 cgctgatggc gcgtgccgga tttgaaaaaa ccgaacgtga taatgtctac aacgggatga
     5401 ccctgcgtga atatgcccgt atgtcactga ctgaacgggg tattggggtt tccagttata
     5461 acccgatgca gatggtcggt gcggcgttca cacacagtac gtctgacttc ggtaatattc
     5521 tgctggatgt tgcgaacaaa gccattctgc agggctggga agatgcccct gaaacctatg
     5581 aacagtggac gcggaaaggt cagttgtctg attttaaaat tgcccatcgt gtgggtatgg
     5641 ggggcttcag tgctctgcgt caggtgcgtg aaggggcgga atataaatac gtcaccaccg
     5701 gagataaaca ggccactatt gcactggcga cctatggcga gctgttcagt atcacccgtc
     5761 aggccattat caatgatgat ctgaatatgc tgaccgatgt cccgatgaaa ctgggccgtg
     5821 cggcgaaatc cactattgcc gatctggttt atgccattct gacgtctaac ccgaaaatat
     5881 ccacagataa tgtaagtctg ttcgataaag cgaaacatgc aaacgtactg gagagcgctg
     5941 caatggacgt ggcatcgctg gataaagccc gccagttgat gcgcgtacag aaagaggggg
     6001 agcgtcatct gaatattcgt cctgcgttcg tactggtacc gacggcgatg gagtctgttg
     6061 ctaaccaggt cattcgctcc tcaagtgtca agggggctga cattaacgcc ggtattatta
     6121 acccggtgaa agattttgcg accgttattg cagagcctcg tcttgatgat aacagccaga
     6181 ccaccttcta cctggctgcg tcaaaaggct ccgatacgat tgaagtggct tatctcaacg
     6241 gtgtggatac gccatatatt gatcagatgg agggcttcag tgtggatggc gtgacaacga
     6301 aagtgcgtat tgacgctggt gtcgcgccag ttgatcaccg cggtctggtg aaatgtacgg
     6361 cgtaaacgtc gcagataaca actctgatgg cccgtaaggg ctttttttgt acctgaaatc
     6421 agcccctgaa cggggctgtg cggagacagt tatggcaaag aattttgtag aagaaggaaa
     6481 aacggtggcg attgttgcca gtgcagccat cagcagcgga gatctggtgc aggtggggga
     6541 tgtttttgcg gtggcgctga ccgatattcc acagggtgaa acaggcgacg gcctgaccga
     6601 aggtgtgttt atgctgccta agctgaaaac ggatgacatg aaaacgggta agaaggttta
     6661 tctgaagtcc ggaaaagttc agctgactaa cagcggctct gatccgctgg tcggggttgt
     6721 ctgggcagat gccggaacca gtgcagaaga agtgccggta aaactcaatg tctgatccct
     6781 tttcccggct ggcagcgcgt atggatgcga tcacggtcag aaagatggga aagacagcct
     6841 cgattaatga tgtcgatatg actgtgatcc cgggagaaac actggcagag ctgaatgctc
     6901 tgtccggacc tgcggtctct ctggtggtgt tttcttcggg ataccgccca cggcgcgggg
     6961 atcgcgttgt ttatgacgga caacaatgga cggtcacacg gcatgaacgc tttaacggta
     7021 agccaatgat ctttattgag taaagaggtg tgggatgaag gggcttgaga atgccatccg
     7081 caatctgaac agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg
     7141 tgtggcacag aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg
     7201 agataaccag gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa
     7261 agccagtccg ttaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcctgc
     7321 cattaagctg gggacagccc gggtcagact gacccggcgt ggtggaaaac tgcagtaccg
     7381 tggcatcgtg ctgaaggtgg gtaaatatct tttccgggat gcgtttattc agcaactggc
     7441 gaatggtcgc tggcatgtga tgcggcgtat tgatggcaaa aatcgttacc ccattgatgt
     7501 ggtgaaaatc cctctgtccg gaccgctgac acaggcattt gaagatgccc gcgaccgcat
     7561 cattgctgcg gaaatgccga aacagctggg gtatgcactg aaacaacaac tgaggttatg
     7621 gctgacccga tgaaccgaca tacacaaatc cgccaggtcg tactggcacg ccttcgggaa
     7681 cagtgtggag acagcgccac gttttttgac gggcttccgg catttgttga tgcgcaggaa
     7741 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgaa
     7801 gatgactggc aggctgttct gcatattgct gtcttcatcc gggcacaggc accggattca
     7861 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     7921 tccggactca tcgacaccct gatcccactc ggttttaact atcaacgtga taatgagatg
     7981 gccacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     8041 aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tctggatgta
     8101 caccggcaag ggcgatgcgt ttgcgaaccc tttatcggac actgactggc tgcgtcttgc
     8161 gatggtgaag gatctgcaac ctggcgaaat gaccgctgat gcagaagatg acacttatct
     8221 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac
     8281 ttcggcgacg ctggcctggc gtccgggtga cagcggacag aaaagactgg ttcagttgtt
     8341 cgactccggt gaagtctgcg cgtttcgaat caaatatccc aacggtactg ttgatgtttt
     8401 ccgtggctgg ctgagttcac tgggtaaaac cattgcctca aaagacgtga tgacccgcac
     8461 agtgaaaatc agcggtgtgg ggcgtccgta tctggcagag gagggcactg aaaccgtggg
     8521 cgttaccggg ctgacggtgg caccggcatc cgccagtgtc aatgtgggag caaccaccac
     8581 gctgaccttt acagtaaaac ctgacggagc cagtgacaaa gcgatcagtg tgcattcgac
     8641 agatccacag acagccactg tgaccctgaa cgggcttgtg gccacggtga aaggcgtgaa
     8701 gcagggcagt gtcagcattg tgggcatgac ttctgacggc gattttgtgg cagtggctac
     8761 ggtggctgtc agcgccgcag gttaacagga cgatactcat catttgcccc ggttatccgg
     8821 ggcttttttg caggtggaga acatgatgtt tctgaaacag ggcacgttta attatgaaaa
     8881 acagtccgtg gtgctcagtg agctgtccgg gctgcagaga attgaatatc tggcgtttct
     8941 tcagcagcga acggcaaagt ttgatgccga agagggagaa ctgccggagg ctgaacgaca
     9001 gattgctttt ctgcggatgg ggatggatat caatgcctgg ctggtttccc gctcactgtg
     9061 gaatgcggat cagtctaagg atgtagagac gctttgcgca tccgttatta caacatggtc
     9121 gtatgatgcc ctgggtgcgg gggcggagat ggttctgtcg ctgagcggta tgggggccat
     9181 tgagaatgcc ggggatttgg agcatgaggt gctgacgccg gaaaagtcct gacgcgggaa
     9241 atgcagtttg tcatgcggct tgcccgggag ttccggcggg cagactggcg gcggatgctg
     9301 tcggaaatgt cggccactga gcttggtgag tggggcgatt atttccggat gcagagcttc
     9361 agtgatgtgt ggatggatgc gcagtttgcc tcgctgaagg cattgatcgt gagaatggtg
     9421 tccggcagca gtgatgctgc ggtggctgat ttcagccttt taccggaaga gaacgggata
     9481 ccggagcgaa cggacgaaga actgatgcat cttggggaag gtatttccgg aggtgtgcgt
     9541 tatggaccag atagccaacc tggtcattga tttggggatt gatgcggcag agtttaaaaa
     9601 tgaaattccc cgtatcaaaa accttctgaa tggtgcagcc agcgatgcag aacggtcttc
     9661 tgcccgtatg cagcgtttta tggagcgtca gactcaggcc gcccggcaga caacgcaggc
     9721 ggcttcttcg gctgcaacag ccgcatccgt ccatgcgcag acggtggaga agaacgcaca
     9781 ggctcatgaa ctcatggccc gcgaggtgga gaaaacccgc cagcgcatgg aggcactgag
     9841 ccagaaaatg cgcgaggaac aggcgcaggc catggctctg gcggaggctc aggataaagc
     9901 ggctgctgcg ttttatcgtc agattgacag tgtgaaacag gccagtgcgg ggctgcagga
     9961 attacagcgt attcagcagc agatccgaca ggccagaaac agtggcggga ttggtcagca
    10021 ggattatctg gcgctgattt ctgaggttac tgcgaaaacc cgtgttctta cacaggctga
    10081 ggaagaggct acccgacaga aagtggcgtt tatccgtcag cttaaagagc aggcaacccg
    10141 cctgaatctt tcatcttctg agttgcttcg tgctaaggca gcccagctgg gggtaagcag
    10201 tgctgcagaa gtgtatatcc gcaaaatgga gcaggcagga aaagccacgc attcgctggg
    10261 tctgaaaagt gcagcagccc gccaggagat aggcgttctg ataggtgaac tggcccgcgg
    10321 caatttaggg gcgctgaggg gatccgggat aacgctggct aaccgtgccg gatggataga
    10381 cacactgatg tcaccgaaag gcatgatgct tggcggggtt attggcggta ttgccgcggc
    10441 cgtctatggt ctgggtaaag cctggtatga cggtcagaag gagggggaag aatttaaccg
    10501 ccagttgtcg ctgacggggc attatgccgg agtcactgcc gggcagctgt ggacgctcag
    10561 tcgtgctatt tccgggaatg gtatcacgca acatgctgca gccggtgcgc tggctcaggt
    10621 ggtggggagt ggtgcatttc gtggaaacga tatcggtatg gtggcgagag ctgccgcaca
    10681 gatggagcga tcggttggcc agtcggtcag cgataccata aatcagttta agcggctgaa
    10741 ggatgatcct gtaaatgccg cgaaggctct ggacaatgag ctgcattttc ttactgccac
    10801 tcagcttgag cagatacgcg tccttgggga gcaggggcgg tccagtgatg ctgcacggat
    10861 agccatgtct gcactggcag aggaaaccgg tcggcgtact gcggatattg ataataacct
    10921 caatgcgctt ggcagtacgc tgaagtatct gtctgatttg tggagtcgtt tctgggatgc
    10981 ggccatgaat attggtcgtg aagactcgct ggatgaacag attgccgctt tacaggagaa
    11041 agtgtcgcgg gcgaaaagac tcccctggac ggcatcatct tctcaggttg aatacgatca
    11101 gcagcgtctt aacgatcttc aggagaaaaa acgccagaag gatttgcagg atgcaaaaga
    11161 gcaggcagag cggaattatc aggagcaaca gaaacgccgt aatgctgaaa atgctgcact
    11221 gaaccggatg aatgaaacgg aagcagcacg acatcagcgt gaaattgcgc gtattaatgc
    11281 catgcagtac gccgatcagg ctgtcaggga tgcggcgata caacgtgaaa atgaacgtta
    11341 cgagaaagcc gtggcatccg gtaagaaaaa aacacgcgaa acccgtaatg atgaggccac
    11401 ccggttattg ctgcagtaca gtcagcaaca ggcacaggtg gaaggacaga ttgctgctgc
    11461 cagacagtca gcaggcatcg ccacggaaag gatgacagaa gcgcataaac agcttctggc
    11521 tctgcagcag cgcatcagcg acctggacgg gaaaaaactg acggcagatg aaaagagtgt
    11581 gctggcccgt aaagatgaac tgattcaggc actgacgctg ctggatgtaa aacagcagga
    11641 gcttcagaaa cagacggcac tcaacgagct gaagaaaaaa acaattcagc tgaccagtca
    11701 actggctgaa gaagagcgcg ctcagcgtca gcaacatgac ctggatatcg ccacggtggg
    11761 tatgggtgat cagcagcgac agcgatatca ggtacaactg agtcttcgcc agaaatacca
    11821 gcaacagctg gagcagttga ggcgggatag tgagcagaaa gggacatata acacggatga
    11881 ctacagaaag gccgagcagg cgctgacgga gagcctgaac cgacaactga atgagaatcg
    11941 ccgttactgg caacagcttg aagttgtgca gggtaactgg aaaaacggag tcctgcgtgc
    12001 atttcaggat tttaccgtgg atgcagataa tacggcagaa acagcagaac aggtgttctc
    12061 gtcagccttc agcaacatgg gaaatggcct ggcaactttt gtcactaccg gcaaactcaa
    12121 tttcaaatcc ttcacctctt ctgtgctgtc agatatggcg aaaatcctgg cgcaggcaac
    12181 catgatgaaa tcgataaaag ggattggcag tgtactggga tttgatctca gcagcctttc
    12241 cctgaatgcc aatgggggga tttatcagtc tgctgatttg agtcgttaca gtggcacggt
    12301 ggttaaccgt ccgacgtttt ttgcttttgc aaaaggcgcg ggtgtgatgg gggaagctgg
    12361 acctgaagcc attctgccac tgcgtcgtgg tgctgacggt aagctggggg ttgtggcgga
    12421 tattggtggt tcaggtatgg cgatgtttgc cccgcagtac aacatcgaga tcaataacga
    12481 tggcacgaac gggcagatag gtccggctgc cctgaaggtg gtttatgacc ttgggaaaaa
    12541 agcggcagcg gactttatgc aacagcaggc ccgtgatggt ggtcggttaa gtggagcata
    12601 tcggtaatgg agacgtttca ctggaaagtg cgcccggata tgaatgtggt atcagagccg
    12661 aaagtggtga cagtgaagct gggcgatggc tatgaacagc gtcgtgcggc gggactgaat
    12721 aaccagttgt cgacttacag cgtgacgata cgtgttcgta aatgtgaaca cccatcttta
    12781 aaagcctttc tggaacggca cggtggcgtc cgcgcatttc agtggacgcc accttatgac
    12841 tggaagccga tcagggtggt ttgtcgtaaa tggtcggcaa gcgtgggggc gttgtgggta
    12901 accataacgg cagattttga acaggtcgtg gcataggagt ctctgatgca ggatattcca
    12961 caggaaacac atcatgagac gacacgcctc actcagtcag cccaggtggt gctctgggaa
    13021 atcgatctga cagtggtcgg tggagaacgt tattttttct gtaatgagca gaacgaaaaa
    13081 ggtgagccgg tcacctggca ggggcgacag tatcaggcgt atcccattca ggggacggga
    13141 tttgaactga acggcaaggg cagtgctgcc cgtccgacac tgacggtttc taacctgcac
    13201 ggcatggtca cggggatggc ggaagacctg cagagtctgg tcggcggaac ggtggtcagg
    13261 cgtaaggttt acgcccgttt tctggatgcg gtgaacttcg tcaacggaaa cagcgacgcc
    13321 gatccggagc aggaggtgat cagccgctgg cgcatcgagc agtgcagcga actgagtgcg
    13381 gtcagtgcct cctttgtgtt gtccacaccg acggaaacgg atggtgctgt ttttccggga
    13441 cgtatcatgc tggccaacac ctgcacctgg acctatcgcg gtgatgagtg cggttatcac
    13501 ggtccggcgg tcgcggatga atatgatcag ccgacgtccg atatcacgaa ggataaatgc
    13561 agcaaatgcc tgagcggctg taagtttcgc aataacgtcg gcaactttgg cggcttcctt
    13621 tccattaaca aactttcgca gtgaatccca tgacagagac agaatcagcg attctggcgc
    13681 acgcccggcg atgtgctcca gcggagtcgt gcggcttcgt ggtaagaacg ccggaggggg
    13741 aaagatattt cccctgcgtg aatatctccg gtgagccgga ggcgtatttc cgtatgtcgc
    13801 cggaagactg gctgcaggca gaaatgcagg gtgagattgt ggcgctggtc cacagccacc
    13861 ccggtggtct gccctggctg agtgaggctg accggcggct gcaggtgcag agtgatttgc
    13921 cgtggtggct ggtctgccgg ggggcgattc ataaattccg ctgtgtgccg catctcaccg
    13981 ggcggcgctt tgagcacggg gtgacggact gttacacgct gttccgggac gcttaccatc
    14041 tggcgggaat tgagatgccg gattttcatc gcggggatga ctggtggcgt cacggccaga
    14101 atctctatct tgacaatatg gaggcaacgg gtttttaccg tgtcccactg acagaggcgc
    14161 agcctggcga cgtgctgctg tgctgttttg gttcatcggt gccgaatcat gccgccattt
    14221 actgtggcga cggcgagctg ctgcaccata ttcctgaaca actgagtaaa cgagagaggt
    14281 acaccgacaa atggcagcga cgcacacact ccctctggcg tcaccgggca tggcacgcat
    14341 ctgcctttac ggggatttgc aacgatttgg tcgccgtatc gaccttcgtg tgaaaacggg
    14401 ggctgaagcc atccgggcac tggtcacaca gctcccggcg tttcgtcaga aactgaatga
    14461 gggctggtat cagatacgga ttgccgggcg tgatacaggt gaaaacgaat tatcagcccg
    14521 tctgaatgag ccgctggaaa atggtgccgt gatccacatc gtgccgcgtc tggcgggagc
    14581 taaaagtggc ggtgtgtttc aggtggtgct gggggcggcg ctgattgcgg tggcatggtg
    14641 gaaccctgtg ggctggctgg gtgccgcggc tgtatcgggc atgtatgcgg caggggccag
    14701 tatgatcctg ggcggagtgg cgcagatgct ggcaccgaaa gccaggacgc ccacggcagc
    14761 aagtacagat aacggcaaac agaacaccta tttctcctca ctggataaca tggttgccca
    14821 gggcaatgtt ctgcctgttc tgtacgggga aatgcgcgtg gggtcacgcg tggtttctca
    14881 ggagatcagc acggcagacg aaggggacgg tggtcaggtt gtggtgattg gtcgctgatg
    14941 caaaatgttt tatgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat
    15001 gggtaaaggc agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac
    15061 gcagctgctg agtgtgattg atgccatcag cgaagggccg attgaaggtc cggtggatgg
    15121 attaaaaagc gtgctgctga acagtacgcc ggtgctggac agtgagggga ataccaatat
    15181 atccggcgtc acggtggtgt tccgggccgg tgagcaggag cagacaccgc cggagggatt
    15241 tgaatcctcc ggctccgaga cggtgctggg tacggaagtg aagtacgaca cgccgattac
    15301 ccggaccatc acgtcggcaa acatcgaccg tctgcgcttt accttcggtg tgcaggcact
    15361 ggtggaaacc acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat
    15421 acagcgtaat ggtggctggg tgacggaaaa agacatcacc attaaaggca aatccacctc
    15481 acagtatcta gcatcggtgg tggtgggtaa cctgccgccg cgcccgttca atatccggat
    15541 gcgcaggatg acgccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc
    15601 atacaccgaa atcatcgatg tgaaacagtg ctacccgaac acggcactgg tcggcgtaca
    15661 ggtggattcg gagcagttcg gcagccagca ggtgagccgt aattatcatc tgcgcgggcg
    15721 tattctgcag gtgccgtcga actataaccc gcagactcgg caatacagcg gtatctggga
    15781 cggaacgttt aagccagcat acagcaataa catggcctgg tgtctgtggg atatgctgac
    15841 ccatccacgc tacggcatgg ggaaacgtct tggtgcggcg gatgtggaca aatgggcgct
    15901 gtatgtcatc ggccagtact gcgaccagtc agtgccggac ggctttggcg gcacggagcc
    15961 gcgcatcacc tgtaatgcgt acctgaccac acagcgcaag gcgtgggatg tgctcagtga
    16021 tttctgctcg gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca
    16081 ggaccgacca tcagataagg tgtggaccta taaccgcagt aatgtggtga tgccggatga
    16141 tggcgcgccg ttccgctaca gcttcagcgc cctgaaggac cgccataatg ccgttgaggt
    16201 gaactggatt gacccgaaca acggctggga gacggcgaca gagcttgttg aagatacgca
    16261 ggccattgcc cgttacggtc gtaatgtcac gaagatggat gcctttggct gtaccagccg
    16321 ggggcaggca caccgcgccg ggctgtggct gattaaaacg gaactgctgg agacgcagac
    16381 cgtggatttc agcgtgggtg ctgaagggct tcgccatgtg ccgggtgatg tcattgaaat
    16441 ctgcgatgat gactatgccg gtatcagcac cggtggtcgc gtgctggcgg tgaacagcca
    16501 gacccagacg ctgacgctcg accgtgaaat cacgctgcca tcctccggta ccacgctgat
    16561 aagcctggtt gacggaagtg gtaatccggt cagcgtggag gttcagtccg tcaccgacgg
    16621 acttaaggtg aaagtgaacc gggttcctga cggcgttgca gaatacagtg tgtgggggct
    16681 gaagttgccg acgttgcgtc agcgcctgtt ccgctgtgtg agtatccgtg agaacgatga
    16741 cggcacgtat gccatcactg ccgtgcagca tgtaccggaa aaagaagcca tcgtggataa
    16801 cggggcgcac tttgacggcg accagagcgg cacggtgaat ggtgtcacgc cgccagcggt
    16861 gcagcacctg accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgctg
    16921 ggacacgccg aaggtggtga agggcgtgag cttcctgctc cgtctgaccg taacagcgga
    16981 tgacggcagt gagcggctgg tcagcacggc ccggacgacg gaaaccacat accgcttcac
    17041 gcaactggcg ccagggaact acaggctgac agtccgggcg gtaaatgcgt gggggcagca
    17101 gggcgatccg gcgtcggtat cgttccggat tgccgcaccg gcagcaccgt cgaggattga
    17161 gctgacgccg ggctattttc agataaccgc cacgccgcat cttgccgttt atgacccgac
    17221 ggtacagttt gagttctggt tctcggaaaa gcggattacc gatatcaggc aggttgaaac
    17281 cacagcccgc tatcttggta cggcgctgta ctggatagcc gccagtatca atatcaaacc
    17341 gggccatgat tattactttt atatccgcag tgtgaacacc gttggcaaat cggcattcgt
    17401 ggaggccgtc ggtcgggcga gcgatgatgc ggaaggttac ctggattttt tcaaaggcaa
    17461 gataaccgaa tcccatctcg gtaaagagct gctggaaaaa gttgatctga cggaggataa
    17521 cgccagcaga ctggatgagt tttcgaaaga gtggaaggac gctaacgata aatggaatgc
    17581 catgtggggc gtcaaaattg agcagaccaa agacggcaaa cattatgtcg cgggtattgg
    17641 cctcagcatg gaggacacgg aggaaggcaa gctgagccag tttctggttg ccgctaaccg
    17701 tatcgcgttt attgacccgg caaacgggaa tgaaacgccg atgtttgtgg cgcagggcaa
    17761 ccagatattc atgaacgacg tgttcctgaa gcgcctgaca gcccccacca ttaccagcgg
    17821 tggcagtcct ccggtatttt ccctgacatc agacggaaag ctgaccgcta aaaatgcgga
    17881 tatcagtggc agtgtgaatg cgaactcagg gacgctcaac aatgtcacga ttaatgagaa
    17941 ctgtcagatt aaggggaaac tgtcagccaa ccagattgaa ggtgatattg tcaaaacggt
    18001 cagcaagtct ttcccccgca cgagcactta tgccagtggc accatcacgg taagaatcag
    18061 tgatgatcag aaatttgacc ggcaggtcat gataccgcca gtgttattcc gcggtggtaa
    18121 gca
//
LOCUS       DAPGWD010000081        18036 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPGWD010000081 DAPGWD010000000
VERSION     DAPGWD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.081"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..366
                     /gene="ecpR"
                     /locus_tag="RHX88_002720"
                     /gene_synonym="matA"
     CDS             <1..366
                     /gene="ecpR"
                     /locus_tag="RHX88_002720"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDV2231390.1"
                     /translation="NRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAGL
                     SRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTVY
                     THRRNAEAKLYSKIYKLVQ"
     gene            441..1028
                     /gene="ecpA"
                     /locus_tag="RHX88_002721"
                     /gene_synonym="matB"
     CDS             441..1028
                     /gene="ecpA"
                     /locus_tag="RHX88_002721"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDV2231391.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1085..1753
                     /gene="ecpB"
                     /locus_tag="RHX88_002722"
                     /gene_synonym="yagY"
     CDS             1085..1753
                     /gene="ecpB"
                     /locus_tag="RHX88_002722"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDV2231392.1"
                     /translation="MKKHLLPLALLFSGIPPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1779..4304
                     /gene="ecpC"
                     /locus_tag="RHX88_002723"
                     /gene_synonym="yagX"
     CDS             1779..4304
                     /gene="ecpC"
                     /locus_tag="RHX88_002723"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDV2231393.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4294..5937
                     /gene="ecpD"
                     /locus_tag="RHX88_002724"
     CDS             4294..5937
                     /gene="ecpD"
                     /locus_tag="RHX88_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDV2231394.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNTDCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYNAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5906..6616
                     /gene="ecpE"
                     /locus_tag="RHX88_002725"
     CDS             5906..6616
                     /gene="ecpE"
                     /locus_tag="RHX88_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDV2231395.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNHTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVRQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6929..7258
                     /locus_tag="RHX88_002726"
     CDS             6929..7258
                     /locus_tag="RHX88_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV2231396.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWTEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7506..8120)
                     /locus_tag="RHX88_002727"
     CDS             complement(7506..8120)
                     /locus_tag="RHX88_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDV2231397.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8538..9227
                     /gene="paoA"
                     /locus_tag="RHX88_002728"
     CDS             8538..9227
                     /gene="paoA"
                     /locus_tag="RHX88_002728"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HDV2231398.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIIPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAALKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9224..10180
                     /gene="paoB"
                     /locus_tag="RHX88_002729"
     CDS             9224..10180
                     /gene="paoB"
                     /locus_tag="RHX88_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HDV2231399.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVPNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIHPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFSSAHPTAENTFKLLLAKRTLASILAEARAQA"
     gene            10177..12375
                     /gene="paoC"
                     /locus_tag="RHX88_002730"
     CDS             10177..12375
                     /gene="paoC"
                     /locus_tag="RHX88_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HDV2231400.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12385..13341
                     /gene="paoD"
                     /locus_tag="RHX88_002731"
     CDS             12385..13341
                     /gene="paoD"
                     /locus_tag="RHX88_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HDV2231401.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            13392..13730
                     /locus_tag="RHX88_002732"
                     /pseudo
     CDS             13392..13730
                     /locus_tag="RHX88_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14019..15296
                     /locus_tag="RHX88_002733"
     CDS             14019..15296
                     /locus_tag="RHX88_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2231402.1"
                     /translation="MRYKPNQYIICDSYGNYYLRITLPVYMQPFFEGKRTFVRSLHTS
                     NLRVARRKRDQIADEYHCLRESVAPVNSTIENTLELLRSKAKYAKTATRMQDTASSCP
                     SLLKILEIYLTINSTKKKPATLAKARKAVEMFLSYRKKPDIALQDVSRTTVTGWIEHM
                     QKTLSQQSIANYISPMAQLWELASSRYHDAPERALSPWRGHRLDVAQSRESYEAFSNK
                     ELLQVLQVFSGDSAENKEMMALCLIGAYTGMRINEIASLTIDDVKEIEGVLCFEITQG
                     KTKAAARVVPVHSLITPLVLSLREKPHNGFLFYHASITERADGKRSTWHTQRFTRAKR
                     KALGEKGTERKVFHSLRHGVAQLLDRNQIPEDRIALLLGHTRGNTETFRTYSKNAASP
                     VELKNYIELLRYPEIEKGLSINKKSNLRRKTTP"
     gene            complement(15377..16090)
                     /locus_tag="RHX88_002734"
     CDS             complement(15377..16090)
                     /locus_tag="RHX88_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231403.1"
                     /translation="MATAGILTIRAANTPEQHVQAVYKAEQDINSTKNENSKANKTKG
                     ENGFAIVTSGLASSGNDVYENYMALAFDSVDDVNVRRSADVTSYPVENGATVSDHVQI
                     KNNKFSLKGRITETPIKSDPGLLKSAGVNGNRRSLAIDYLNQIMDSRQPFLLVTENKT
                     FENVVLTGIEYTEEASESLVFDLEFEQIRLVSYGTVNTVAIKTQPSKNIGANMKKRVN
                     TEKSSSEGEDTITPAFKQE"
     gene            complement(16125..17954)
                     /locus_tag="RHX88_002735"
     CDS             complement(16125..17954)
                     /locus_tag="RHX88_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231404.1"
                     /translation="MLEINTSKIKNTVTFSVDKDSLKKAKDSITGLKEFAENIKPAKL
                     RFDTVTKGYKKAQSEVDKIAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELK
                     LLDVSSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKIN
                     GNRKTQLAPVKGGSGNSATMGALLFGGATAAAGVMAVSKASDFVTNSFANAETQGELI
                     QRAKLGGVDVNQMYNITEWAYKNGVDSMMGDQGARKYLDQMKDVRERAAKSYSEAELV
                     KDKKTGKSEWKGGDNAINELLNIGVINKSDLKKFADNPAGLISKAVNGMMKKGFSDSQ
                     IGQRLEDLGDDLMLTSKYWQRSVKDVQESINQQKASGKWLTETQQDSIVKFRELNRQL
                     SQLSDARQVAFVDGFMKSLDPKVTEEFMKNLSNLTPYFTKLGEAVGSLFDAIMKIVNW
                     FNRNDDKTDAIQKNMGDAPPLSNEGMKQNLSNLVPDQYKGAGTATTTPDNSHSLFNTI
                     KGLLFDDNSSVSDVKMSVNEAPITNLKQGALNNLAMTTPAYNLSPVFNLNPTFEVVTE
                     VPLTINSDTSRLSDYVDFTARASRDSFLKSLTLTSLSGQSNGG"
BASE COUNT         4564 a         4626 c         4531 g         4315 t
ORIGIN      
        1 aaccgtgata atgaatggtt atccgcggta aaggggaaac aggtcgtatt gattgcggcc
       61 agaaagtcag aagccttagc aaattattgg tattacaaca gcaatattag gggcgtggta
      121 tacgcaggac tgagtcgtga tattagaaaa gaactggcct atgtgattaa tggcaggttc
      181 ctgagaaaag atattaagaa agataaaatc acggaccggg aaatggaaat tatccgcatg
      241 acggcccagg gaatgcaacc taaatcgatt gccagaattg aaaattgtag tgtgaagaca
      301 gtgtataccc atcggcgtaa tgctgaggcc aagctgtact caaaaatata taagttggtt
      361 cagtaaactc caggcaagtt agttttaaaa aatgactcac tgggacatca cgtcctcaat
      421 tcaactcggg aagaaatgca atgaaaaaaa aggttctggc aatagctctg gtaacggtgt
      481 ttaccggcat gggtgtggcg caggctgctg acgtaacagc tcaggctgta gcgacctggt
      541 cggcaacagc caaaaaagac accaccagta agctggttgt gacgccactc ggtagcctgg
      601 cgttccagta tgccgaaggc attaaaggtt ttaactcaca gaaaggtcta tttgacgtgg
      661 ctatcgaggg tgactcaacg gctaccgcct ttaaactgac ctcacgtctt atcaccaaca
      721 cattaaccca gttggatacc tcaggttcca cactgaatgt gggcgtggat tataacggcg
      781 cggcagtcga aaaaactggc gataccgtga tgatcgatac cgccaacggc gtactgggcg
      841 gcaaccttag cccgctggct aacggttaca atgccagcaa tcgtaccacc gcacaggatg
      901 gtttcacctt caccatcatc agcggcacca ccaatggtac caccgcagta accgattaca
      961 gcactctacc ggaaggcatc tggagcggcg acgttagcgt acagttcgac gcgacctgga
     1021 ccagttaatc tctctgatgt accagcaggg gtagccccct gctttattcc tggacggaat
     1081 tgttatgaaa aagcaccttc tgcctctcgc tctgctgttt tccggaatac ctccggccca
     1141 ggcgctggat gtcggcgata tatcatcgtt tatgaacagt gacagcagca cgctgagcaa
     1201 aacgatcaaa aacagtaccg acagtggtcg ccttatcaat atccgtctcg aacggctctc
     1261 ttcaccgctt gacgacgggc aggttatctc aatggacaag ccggatgagt tgctactcac
     1321 tcccgccagc ttgctgctac ccgcccaagc cagcgaagtg atccgcttct tctataaggg
     1381 acccgcagat gaaaaagagc gctactaccg cattgtctgg tttgatcagg ccctcagtga
     1441 tgcgcagcgc gataatgcca accgcagcgc tgtggccact gcttccgccc gcatcggcac
     1501 cattctggtc gtcgcccccc gccaggcgaa ctaccacttt cagtacgcca acggctccct
     1561 gacaaataca ggaaatgcga cgctgcggat cctcgcctac ggcccttgcc tgaaagccgc
     1621 caacggtaag gagtgtaaag agaattacta cctgatgccg ggcaagtcgc gtcgttttac
     1681 ccgcgtggac acggcggata acaaaggacg ggttgcactt tggcagggtg ataagttcat
     1741 tcccgtgaaa tagatagctg tgcagatgga taacgacaat gcctttacga cggttctccc
     1801 caggactgaa agcccagttt gccttcggca tggtcttttt gttcgttcag cccgatgcca
     1861 gcgctgctga cataagtgcg cagcaaatag gtggggtgat tattccgcag gccttcagtc
     1921 aggcgcttca ggacggcatg agcgtcccgc tctatattca tctcgccggt agccagggtc
     1981 gccaggacga tcagcgaatc ggcagcgctt ttatctggct ggatgatgga cagctacgca
     2041 tccggaaaat acagctggaa gagagtgaag ataacgccag tgtcagcgaa caaactcgac
     2101 agcagctgat ggctctggcc aacgccccgt tcaatgaggc ccttaccatc cccctgactg
     2161 acaacgcgca gctggatctc agcttgcgcc aactgctgct gcagctggtg gtcaagcgcg
     2221 aagcgctggg caccgtacta cgctcacgta gcgaagacat cgggcagtcc agtgttaaca
     2281 ccctcagcag taatctgagc tataacttgg gcgtctataa caaccagttg cgtaacggcg
     2341 ggagcaacac atccagctat ctgtcgctga ataacgttac tgcgctgcgc gaacatcatg
     2401 tggtgctcga cggctcgctg tacgggatcg gtagcggtca acaggacagt gaattatata
     2461 aagcgatgta tgaacgcgat tttgccggtc accgatttgc cggtggaatg ctcgacacct
     2521 ggaacttgca gtccttaggg ccgatgaccg ccatttcagc agggaagatt tacggccttt
     2581 cctggggaaa ccaggccagc tccaccatct tcgacagcag ccagtcagcc acgccagtga
     2641 tcgccttttt accggcggcg ggcgaagtac atctcacccg tgatgggcgg ctactaagcg
     2701 ttcagaactt cactatgggc aatcatgaag tggatacccg gggtctaccg tacgggattt
     2761 acgatgtgga agttgaggtg atcgttaacg gtcgcgtgat cagcaaacgc acccagcggg
     2821 tcaataagct gtttagccgg gggcgcggcg tcggtgcacc actggcgtgg caggtatggg
     2881 gcggtagctt tcatatggat cgctggtcgg aaaacgggaa aaagacgcga ccagctaaag
     2941 agagttggct agcaggtgcc tcgacctccg gctcattgag tacgcttagc tgggcggcaa
     3001 cgggatatgg atacgataat caggcggtgg gtgaaacccg tctgacgctg ccgcttgggg
     3061 gggcgatcaa cgttaacctg caaaatatgc tggccagtga cagctcatgg agcagcatcg
     3121 gcagcatcag cgccactcta ccgggaggct ttagttcgct gtgggttaat caggaaaaaa
     3181 cccgcattgg caatcaattg cgacgtagcg atgccgataa ccgtgcaatc ggcggcacac
     3241 tcaacctgaa ctcactgtgg tcgaagctgg gcacattcag catcagctac aatgatgacc
     3301 gccgttacaa cagccattat tacacggcag attactatca aaatgtctac agcggtacct
     3361 ttggttcgct tggcctgcgg gccggtattc agcgctataa caacggcgac agcaacgcca
     3421 atacagggaa atatatcgct ctcgatctct cgctaccact gggcaactgg tttagcgcag
     3481 ggatgaccca tcaaaacggc tacaccatgg caaacctgtc agcacgcaaa cagtttgatg
     3541 aaggaaccat tcgcactgtt ggtgccaatc tgtcacgtgc catctccggc gataccggtg
     3601 atgacaaaac cctcagcggt ggggcgtatg cacagttcga cgctcgttac gccagcggaa
     3661 cgctgaacgt caatagcgcg gcggacggct acatcaatac caacttgacc gccaatggca
     3721 gcgtcggctg gcagggtaaa aacatcgctg ccagcgggcg gactgatggc aacgctgggg
     3781 tgatattcaa caccgggctg gaggacgacg gtcagatcag cgccaaaatc aacgggcgga
     3841 ttttcccgct taacggcaag cgtaactatc tcccgctctc tccctatgga agatatgagg
     3901 tggagttaca gaacagcaaa aactcactcg acagctacga tatcgtcagc ggccgcaaaa
     3961 gccatctgac tctctatcca ggcaatgtcg ctgtcattga gccagaggtg aagcagatgg
     4021 ttaccgtctc cggtcgtatc cgtgcggaag acggcacact gctggctaac gcacggatta
     4081 acaaccatat cggccgaacc cgaaccgatg aaaacggcga gtttgtcatg gacgtggata
     4141 agaaatatcc cactatcgat tttcgctaca gtggcaataa aacctgcgaa gtggcactgg
     4201 aactcaacca ggcgcgcggt gccgtctggg tcggtgatgt ggtctgcagc ggcctctcat
     4261 cgtgggcggc ggtgacgcag acaggagaag agaatgagag ttaacctact aataacgatg
     4321 ataattttcg cgctaatctg gccagtaact gagctcagag cggcagtgag caaaacaacc
     4381 tgggcggatg caccggcacg cgagtttgtg tttgtcgaaa acaactcaga cgacaacttt
     4441 ttcgtcactc ctggcggggc gctggatccg cgcctgaccg gtgccaaccg ctggaccggt
     4501 ttaaaataca ctggttcagg aaccatctat cagcaaagcc tcggctacat tgataacggt
     4561 tacaacaccg gcctttatac caactggaag tttgatatgt ggctggaaaa ttcaccagtt
     4621 tcatctcctt taactggctt gcgctgcatc aactggtacg ctgggtgtaa tatgaccacc
     4681 agtcttatcc tgccgcaaac caccgacgcc agtggatttt atggcgcgac ggtgaccagc
     4741 ggcggcgcga agtggatgca cggcatgttg tcagacgcgt tttaccagta tctgcaacaa
     4801 atgcccgtcg gcagcagctt tacaatgacc atcaatgcct gccagacctc tgtgaactat
     4861 gacgccagca gcggcgcacg ctgtaaggat caggcctccg gcaactggta tgttcgcaac
     4921 gtcacccata cgaaagcagc aaatctgcgg ttaataaata cccactcgct ggcggaggta
     4981 tttatcaaca gcgacggagt accgactctg ggcgaaggga acaccgactg ccggacacag
     5041 accatcggca gcctttcagg attaagttgt aagatggtca actataccct gcaaacaaac
     5101 ggactcagca acacctcaat ccatatattc ccggcgatcg ccaactcgtc gttagcctcg
     5161 gccgtcgggg cgtacgatat gcagttcagt ctgaatggca gttcatggaa accggtgagc
     5221 aataccgcct attactacac cttcaacgag atgaagagcg cagactcgat ctatgttttc
     5281 ttctcgagca acttctttaa gcagatggtg aacctcggga tcagcgatat caacaccaaa
     5341 gatctattca actttcgctt tcagaacacc acatcaccgg agtctggctg gtatgaattt
     5401 tctacctcca acacgctgat tatcaaaccc cgtgatttca gcatcagtat tatctccgat
     5461 gaatatactc agacaccgtc gcgggaggga tatgttggca gcggcgagtc ggcactcgat
     5521 ttcggctata tcgtaaccac cagcggtaaa acagctgccg acgaagtgct gatcaaggtg
     5581 accggacccg cgcaggtgat tggcgggcgc tcctattgtg tcttcagctc cgatgacggt
     5641 aaggcgaaag taccgttccc ggcgacactt tcctttatta cccgcaacgg agctacaaaa
     5701 acctacaatg ccgggtgcga tgatagctgg cgggatatga ccgatgcgct gtggttgacc
     5761 acaccgtgga ctgatatctc tggcgaagtg gggcagatgg ataagaccac agtcaaattt
     5821 tcgattccaa tggataacgc catttcgctg cgtacggtag atgataacgg ctggttcggc
     5881 gaagtcagcg cttcaggaga gattcatgtt caggcgacgt ggcgtaacat taactaaggc
     5941 cctgctgaca gcggtctgta tgctggcggc acctttgaca caggcgattt cggtcggcaa
     6001 tctgacattt tcgctgccgt ccgagactga ctttgtcagc aaacgtgtag tgaataacaa
     6061 caaaagcgca cggatatacc gtattgccat cagtgctatt gatagcccgg gcagcagtga
     6121 attgcgcacc cgaccggtgg atggtgaact gcttttcgcc ccccgccagc tggcgttgca
     6181 ggctggtgag agcgagtatt ttaaatttta ctatcatggt ccacgggata accgcgagcg
     6241 ctactaccgg gtctcatttc gcgaggtccc cactcgtaac cacacaagac gcagcccaac
     6301 cggcggcgag gtcagcacgg agccggtggt ggtgatggat accattctgg tagtacgacc
     6361 acgtcaggtt cagtttaaat ggtccttcga caaggtgaca ggaacggtaa gtaacactgg
     6421 caacacatgg tttaagctac tgattaagcc aggatgtgat tcgaccgaag aggaaggcga
     6481 tgcctggtat ctacgtccgg gagacgtggt tcgtcagcct gagttacgtc agccggggaa
     6541 tcattatctg gtctataacg acaaattcat taagattagc gattcctgtc cggctaagcc
     6601 cccttcggcg gactaagact tatccatcgg tcgagggaag aattccacct cagagctcca
     6661 aaaatcgttt aaatgatgtg gaagcgatcg tcaatgtcac ctgtaacgat gggctttctc
     6721 atatgcacac catagtgttc aggagacacg gaaaggtatc aggcagcctg ctaatgagtg
     6781 gtttaatcac gaattagtac gtaaaatcgg taacggctgg aaatcattca atactcacac
     6841 tatcgaaagt tccccagcca accgcggtac gttcttacat acgatgtacc gctgttctct
     6901 ttacgatttt tagctgtact ggtgaattat gagcaatctg aatccatgca tgacgtgtgg
     6961 tgcctgttgt gcatttttcc gcgtctcttt ttactggacc gaagccgacg atgctggcgg
     7021 tactattccc gccaggctca ctgaacaaat atcccctttt caccgatgca tgagcggtac
     7081 caatcagaaa aacccccgat gtattgccct tgcaggaacc ccgggcaaaa atgcctgctg
     7141 cacgatatat aaaaatcgat cgtccacatg cagagaattt gccatgtctg gtgagaacgg
     7201 agtcgtcaat gaggcttgca atcgtgcaag ggctaaatac gggctgacac cgttataaac
     7261 atacaataat taattgcact gccccgccag cgataatagc ggggcttcgt ttttcagggg
     7321 taacaaaacc cgatacttct tctatttgcc agcaacaatg cctctcttct gtagcgttct
     7381 tcggatctac atcatcctga agtagcgcga tatactcact gactatctga atcagaatat
     7441 aaaaagcaat gtttttaacc tataaaaatg gcgctgtatt tgcgccattt ttatcattca
     7501 atgcattatc tgtttgagcc taaagggatc tcagggtctg gctcatgagt aattctgttt
     7561 cgtaaatctc tgcgaataat ttcaatagac cagaaccaga ctaaatgtcc aaaaatttca
     7621 gaaacattct cataccacgg gagatcaaac agaggtggcg tcagtcccat gagagggaat
     7681 gaaatcatat gaacaaaaag ttgggctaaa gcacctgcca gtaagccctg ccagagttta
     7741 atttttggaa atacttcagc gaccacacaa taaccgacag caaacactat cgaaaagata
     7801 atgtgcgtaa caccaaccca gttaaagaca tgcccggcaa aggtataaac agccgcattg
     7861 ggatctgtca gccccaacca gtctcgaaga aaaatatacg gtggattgag aaaattacgc
     7921 gagcaatcaa tttggcctgc agccctgatt aatgattccg ggccacacgc tgcattaaac
     7981 atatccaccg ggctacgtgg cggcaatgga acttcagccc cccacttcac gaatgcggaa
     8041 acaacgccag caatcagccc aatgaatgca gcaagaccat aacgtctgcg gttcggtgga
     8101 gtttgttcaa atatattcat atctaccctg cttgtaccat tatgttatac acctcttcag
     8161 gagtattcat aaaacaaggc aaatgtaaag aactgtattg ttttgtataa caagatagtt
     8221 tcctaatcgc caatgaatat aagctccatc atttctccct atttttatat tagaagtgac
     8281 agagatttgc agggtgatgc agagctgaaa atcacaggtt tccttattgg tttttgcatc
     8341 gtacaactaa agcaataaac cagctcccat catcatcaac atcccggcga acatgaattt
     8401 attagccggg ctcatagctg cattcgcgtt ttaagaataa tcctcctgct gtcgccgact
     8461 atgcttaacg tttaaaaaag catcagcact ctcgcaacgc actcttattt tcccctttag
     8521 aataccggag gcctggtatg agcaaccaag gcgaataccc cgaagataat cgggttggga
     8581 agcacgagcc gcacgattta agtttgaccc gtcgcgatct gattaaagtg agcgccgcaa
     8641 cagcggcgac cgccgtggtt tatcctcatt ctacgctggc ggcaagcgtt ccggcagcta
     8701 cacccgcgcc agagataata cccctgacac tgaaggtgaa cggcaaaacc gagcagcttg
     8761 aggtggatac ccgaaccacg ctactggacg ctttgcgtga aaatctgcat ttgatcggta
     8821 ccaagaaagg ttgcgatcac ggacagtgcg gagcctgtac cgtgctggtc aatggtcgca
     8881 ggcttaatgc ctgcctgacg cttgcagtca tgcatcaggg ggccgagatc accaccattg
     8941 aaggcctggg ttcgccagat aatcttcacc ccatgcaggc ggcctttatc aagcatgatg
     9001 gcttccagtg cggctactgc acctccgggc aaatttgctc atcagtagcg gcgctaaaag
     9061 agattcagga cggcattccc agtcacgtca cggtcgattt ggtttccgct ccagaaacaa
     9121 ctgccgatga gatccgtgaa cgtatgagcg gcaacatctg tcgctgtggt gcatacgcta
     9181 acatccttgc cgccattgaa gatgctgcgg gggagataaa atcatgaagg cgtttaccta
     9241 tgaacgagtg aacaccccag ccgaggcggc acttagcgct cagcgcgtac ccggcgcaaa
     9301 atttatcgcg ggcgggacca atctgctgga cctgatgaag ctggaaattg aaacgcccac
     9361 ccaccttatc gatgtgaacg gcctcgggct cgataagatt gaagtgaccg acgcgggtgg
     9421 gctgcgcatc ggcgcactgg taccgaacac cgacctggcg gctcacgagc gcgtgcgtcg
     9481 tgattacgcg gtactctccc gcgccctgct cgctggcgca tctggtcagt tacgtaatca
     9541 ggcaaccacc gcaggtaatc tgctccagcg cacgcgctgc ccctattttt acgacaccaa
     9601 tcagccctgc aataagcgcc tgcccgggag cggctgcgcg gcgcttgaag gctttagccg
     9661 tcagcacgcg gtggtaggcg taagcgaagc ctgcattgcc acccatccga gcgatatggc
     9721 ggtcgcaatg cggttgctgg atgcggtggt ggaaaccatc acgccggagg gaaagactcg
     9781 cagtatcaca ctggctgatt tttatcaccc tccgggaaaa acgccgcaca ttgaaaccgc
     9841 cctgcttccc ggtgagctta tcgttgcggt gacgttacct ccgccgctcg gcggaaaaca
     9901 tatctaccgt aaggtgcgcg atcgcgcctc ctacgccttt gccctggtat cggtcgcggc
     9961 gattattcac cctgacggca gcgggcgcgt cgcgctgggc ggagtagcac ataagccctg
    10021 gcgcattgag gctgcggatg ctcagctatc ccagggggcg caggccgtat atgacgcgct
    10081 gttctccagc gcccatccca ccgctgaaaa cacctttaaa ctcctgttgg cgaagcgaac
    10141 gcttgcctcc atactggctg aagcgagggc acaggcatga aatttgataa acccgcaggg
    10201 gaaaacccga tcgatcagct gaaggttgta ggtcgtcccc atgaccgcat cgacggaccg
    10261 cttaaaacta ccggcacggc acgctacgcc tacgaatggc atgaagaagc ccccaacgcc
    10321 gcctatggct atatcgtcgg ttccgccatt gccaaaggac gcctcaccgc ccttgatacg
    10381 gacgccgcgc aaaaagcgcc gggcgtactg gctgtcatta ccgccagtaa cgccggggca
    10441 ctcggcaaag gcgacaaaaa caccgccaga ctgttaggcg gccccactat tgagcactat
    10501 catcaggcca ttgcgctggt agtggccgag accttcgaac aggcgcgagc ggcggcctcg
    10561 ctggtgcagg cacactatcg ccgtaataaa ggagcttact ccctggcgga cgaaaaacag
    10621 gccgtcaatc agccgccgga agacacgccc gacaaaaacg tcggtgactt tgacggggct
    10681 ttcacctccg ctgcggtgaa gattgatgct acctacacga ccccggacca gagccatatg
    10741 gcgatggagc cgcatgcctc gatggccgtc tgggatggaa ataagcttac cctctggacc
    10801 tcaaatcaga tgattgactg gtgccgcacc gatctggcaa aaacgctgaa agttcccgtg
    10861 gagaatgtgc gtattatctc cccgtatatc ggcggagggt ttggcggcaa gctgttcctg
    10921 agaagcgatg cgctgctggc ggccctcgcc gcccgagcgg tgaaacgtcc ggttaaagtg
    10981 atgctccccc gcccctctat tcccaataac accacgcacc gccccgccac ccttcagcac
    11041 ttgcgtatcg gtgccgacca gagcgggaaa atcaccgcta tctcacatga aagctggtct
    11101 ggaaacctgc ccggcggcac gccggaaacg gcggtacagc aaagcgaatt actctacgcc
    11161 ggggcgaatc gtcataccgg cctgcggctc gccacgcttg atttgccgga agggaacgcc
    11221 atgcgtgcgc ccggcgaagc ccccggtctg atggcgctcg aaatcgcgat cgacgaactg
    11281 gcggaaaaag cgggcatcga tcccgtcgag tttcgcatcc tgaatgacac tcaggttgac
    11341 cccgccgacc cgacgcgctg cttctctcgc cgtcagctta tcgagtgctt gcgcaccgga
    11401 gcggataaat ttggctggaa gcagcgcaac gccacacccg gacaggtgcg cgacggggag
    11461 tggctagtcg gccacggtgt tgcggcgggc tttcgcaata atctgctgga aaaatcgggt
    11521 gctcgggttc acctcgaaca aaacggcacc gttaccgtag aaacggacat gaccgacatt
    11581 ggcaccggca gctacaccat tctggcccag acggcagcgg aaatgcttgg cgtaccgctg
    11641 gagcaggttg cggttcacct cggcgattcc agtttcccgg tttctgcggg ttctggtgga
    11701 caatggggcg cgaatacctc cacctccggc gtttacgccg cctgtatgaa gcttcgcgaa
    11761 atgattgcct cggcagtcgg gtttgatcct gagcagtcgc agtttgccga cggcaagatt
    11821 accaacggta cccgaagcgc cacgctacat gaagccaccg caggcggcag actgacagcg
    11881 gaagagagca ttgaattcgg aacactgagc aaagagtacc agcagtcgac ctttgccggg
    11941 cattttgtgg aggtcggcgt gcatagcgcg acgggagaag ttcgggtccg gcgtatgctc
    12001 gctgtgtgtg ctgcaggacg catcctgaat ccgaaaactg cgcgcagcca ggtcattggc
    12061 gcaatgacta tgggcatggg tgcggcactg atggaggagc tggcggtgga tgaccgtttg
    12121 ggctacttcg ttaatcacga tatggcgggg tatgaggtgc cggtccatgc ggatatccca
    12181 aaacaggagg tgattttcct ggatgatacc gaccccatat cctccccgat gaaggccaaa
    12241 ggtgtcggtg agctgggcct gtgcggcgtg agcgcggcta tcgccaacgc ggtgtataac
    12301 gccaccggta ttcgggtacg ggattatccc atcactctgg ataagctgct cgataagctg
    12361 ccggatgtgg tttaaggaga aacaatgtca tacccgcttt ttgataaaga cgaacactgg
    12421 cataagccag agcaggcgtt tctcaccgat gaccaccgga ccattctgcg cttcgccgta
    12481 gaggcgctaa tgtccggtaa aggagcggtg ctggtgacgc tggtggagat acgcggcggc
    12541 gcagcgcgcc cgctcggggc gcagatggtg gttcgcgaag atggtcgtta ctgcggtttt
    12601 gtctctggcg gctgcgtgga ggccgctgcc gcttttgaag cgctggagat gatgggctca
    12661 ggccgcgatc gcgaaattcg ctatggcgaa ggttcgccgt ggtttgacat cgttctgccc
    12721 tgcggcggtg ggatcacgct gacgctccat aaactacgct cggcacagcc tctgctcgcc
    12781 gtgctgaacc gtctggaaca gagaaaaccg gcggggctgc gctacgatcc gcaagcacaa
    12841 tcgctggtgt gcctgcccac gcaaacccga acgggctgga atctcaatgg ctttgaggtg
    12901 gggttcaggc catgcgtcag gctgatgatt tacggacgtt ctcttgaggc gcaggcaacc
    12961 gcgagtcttg cagcagccac aggctatgac agccatatct tcgatctttt tccggcctca
    13021 gccagcgctc agatcgatac cgatacggcg gtcattttgc tgtgccatga tctcaaccgg
    13081 gagctgccag tgttgcaggc cgcgcgagaa gcaaaaccct tttatctcgg cgcattgggc
    13141 agctatcgaa cccacacgtt acgtctgcaa aagctccacg agctgggatg gtccagggag
    13201 gaaacaacgc aaatccgggc acccgtcggg atattcccca aagcccggga tgcgcatact
    13261 ctggcactct ccgtgctggc agaagtcgcc tctgtacgtc tccatcagga ggaggattca
    13321 tgcctgcccc cgtcgtcctg atccttgcgg ccgggcgtgg agagcgcttt ctcgcctccg
    13381 ggggaaatac ccataagtgt atcggctggc gtcagtcccc ggaggttgcg ccttatcgct
    13441 ggccatttga agaaaacggg agaactttcg accttgcgat tgaaccgcag attacgacta
    13501 atgatctgcg tctgatgttg aggctggctc ttgccggcgg aggaataaca attgccactc
    13561 aggaaacttt caggccatat attgaaagcg gtaagcttgt atcgctgctt gatgactttc
    13621 ttccacaatt tccgggcttc tatctgtatt tcccacagcg tcgcaatatt gcaccaaagc
    13681 tccgcgccct gattgactac gtcaaagaat ggcggcagca attggtttaa atagctgcac
    13741 ctgcactgcc tgatgtcaga acagtatttt gatgaattgc caaggttaca atggcacaaa
    13801 tgcggcacag gaggaaacgt ggtgtattta aatatggggt aactctttga ttttaatggt
    13861 gccgataata ggagtaaaac actcgattaa tctcgtgtta atgccatcac ctacgaccat
    13921 tactaattga ttgaatctta tagaaaaaat catcattttc tatgcgttac aatcaatttt
    13981 cacattacaa acgctccgac actttagccg gggagtttat gagatacaag cccaatcaat
    14041 acataatctg tgattcttac ggtaactact atttgaggat cacgttgcct gtgtatatgc
    14101 agcccttttt tgaaggaaaa aggacgtttg tcaggagtct acatacaagt aatcttcgtg
    14161 ttgcacgtag aaagcgtgat cagattgcag atgaatacca ttgtctacgg gagagtgtcg
    14221 cccctgtaaa cagcacaata gagaacacgc tggaactgtt acgcagtaag gctaaatacg
    14281 ccaaaacagc taccagaatg caagatacag cgtcttcgtg tccgtcattg cttaaaattc
    14341 ttgaaatcta cctgacaatt aacagcacga agaagaagcc agccacttta gctaaggcaa
    14401 gaaaagcggt agagatgttt ctctcctacc gtaaaaagcc tgatattgca ttgcaagatg
    14461 taagccgcac cactgttaca ggctggattg aacacatgca aaaaaccctt tcacaacaat
    14521 caattgcaaa ttatatcagc ccaatggccc agctatggga gttagcttca tcacgttacc
    14581 acgatgcgcc agaaagggcg ctctccccct ggcgagggca taggcttgat gtggcacaaa
    14641 gtagagagag ctatgaggca ttttctaaca aagagctatt gcaagtgttg caagtatttt
    14701 ccggtgattc agcagaaaac aaagaaatga tggctttgtg tcttatcggt gcttatacag
    14761 gtatgcggat caatgagata gcaagtctca caatagacga tgtgaaagag atcgaaggtg
    14821 tgctgtgttt tgaaatcaca cagggaaaga cgaaagctgc ggcacgtgtt gtgcctgtac
    14881 atagccttat cactccattg gtgttatctc ttcgtgaaaa gcctcataat ggctttttgt
    14941 tctatcacgc cagcattaca gagcgtgctg atggtaaacg ttccacgtgg cacacgcaga
    15001 gatttacaag agctaaacga aaggctttag gggaaaaggg aacagaaagg aaagtgtttc
    15061 attctctgag acacggagta gcacagcttc ttgatcgaaa tcaaattcca gaagacagga
    15121 tcgcccttct cctgggccat acacgcggta atacagagac attccgcaca tatagcaaga
    15181 atgcagcttc tccagtagag cttaaaaact atattgagct tctacgctac cctgaaatag
    15241 agaaaggctt atcaatcaat aaaaaatcaa atttaaggcg taaaacaacg ccataggcgc
    15301 aataaagagg cttcaacata attggtagcc tcttttataa ttacatcgtt acagagcgtt
    15361 ttagaggcat ttacggctat tcctgtttaa aggcaggagt aatagtgtct tcaccttcac
    15421 tacttgattt ttcagtgtta acacgttttt tcatattagc cccgatgttc ttagatggct
    15481 gtgttttgat agctacagtg tttacagtgc cataagacac taatctgatc tgttcaaatt
    15541 ccaaatcaaa caccagggat tcgcttgcct cttctgtgta ttcgatgcct gttaaaacaa
    15601 cgttctcaaa agttttattt tctgtaacaa gaagaaaagg ctgtctactg tccataatct
    15661 gattcaggta atcaatagcc aatgaccttc tgttcccgtt cacccctgca ctctttaaca
    15721 agccaggatc gcttttaatc ggtgtttcag tgatacgccc tttaagagag aacttattgt
    15781 ttttaatttg aacgtgatca gacacagtag caccattctc tacggggtag cttgtaacat
    15841 ctgccgaacg tctcacgttc acatcgtcaa cactgtcaaa ggccagtgcc atataatttt
    15901 cataaacgtc attgccagaa gaagccagcc cactggtaac aatagcaaat ccattctctc
    15961 cttttgtttt attagctttg ctattttcgt tttttgtaga atttatatct tgttctgctt
    16021 tgtaaacagc ctgaacgtgt tgttctggag tatttgctgc acgtatggtt aaaatcccgg
    16081 cggtcgccat aataataatt ttccttgtaa tgttatggcg ggaattaccc gccgttagat
    16141 tgtcctgata agctggttaa tgttaatgat ttcaagaagc tatcccgact tgctctcgct
    16201 gtaaaatcca cataatcgct taatctggat gtgtcagaat taatagtaag aggaacctct
    16261 gttaccactt caaacgttgg attcagatta aacacaggag ataaattata agccggagtt
    16321 gtcattgcaa gattatttaa agccccctgt ttgagattgg ttatcggtgc ttcattgact
    16381 gacattttta catctgacac agaagaatta tcatcgaaaa gcaatccctt aatcgtgtta
    16441 aagagagaat gactattatc aggtgtagtt gttgcagtgc cagcgccttt atattgatca
    16501 ggaacgagat tagaaagatt ctgtttcatt ccttcattgc ttaatggcgg tgcatctccc
    16561 atatttttct gaatagcatc tgtcttatca tcattacgat taaaccagtt tacaatcttc
    16621 ataatagcgt caaagagact tcctacagct tcccctagct tcgtaaaata cggagtgaga
    16681 ttagaaaggt ttttcataaa ctcttccgtt acttttggat cgagagattt cataaagccg
    16741 tctacgaatg ctacttgtct tgcatcagaa agctgcgaaa gctgtctgtt tagttctctg
    16801 aatttaacaa tagagtcttg ctgtgtttct gttagccatt ttccagaggc tttttgctga
    16861 ttaatacttt cttgtacatc tttaacggaa cgttgccaat attttgatgt aagcatcaaa
    16921 tcatcgccca ggtcttctaa acgttgtcca atttgggagt ctgaaaaccc ttttttcatc
    16981 attccattaa cagccttaga aattaatcca gccggattat cagcaaattt tttaaggtcg
    17041 cttttgttaa taacaccgat gtttagaagt tcattaatag cattatcacc gcctttccat
    17101 tcagattttc cggttttctt gtctttcacc aattcagctt cactatagga ttttgcggca
    17161 cgttccctaa catctttcat ctgatccagg tatttcctag ccccttgatc acccatcata
    17221 gagtcaacgc cgtttttata cgcccattct gtaatattat acatctggtt aacgtctaca
    17281 ccgcccagct tggcccgttg aattagctcc ccttgcgtct ctgcgttagc gaagctgtta
    17341 gtaacaaaat cagaggcttt gcttacagcc atcacgccag cagcagccgt agcgcctcca
    17401 aataacagag cgcccatagt ggcggaattc cctgatcctc ccttaacggg ggctaattga
    17461 gtttttctgt tgccattaat cttacgttgt tgttgttgta ggcgtttcat ctggctattc
    17521 atacgtgcaa gcgagatagc cccttgctca tattgttttg ctatctctct agcctgtgca
    17581 atagctttgt attgttccgc tacagagaga cggtgcattg cactgataga gcttgacaca
    17641 tcaaggagct ttaattcagc tttctcctta cgtgctgcta tttgttgctg ttgccttgcc
    17701 tgtgctcttg ctgcggcagc ttgtgctctt tgattctgct tttctgtttt ggctttctgt
    17761 tgagcaatct tatctacttc actttgtgct ttcttatagc ctttagtgac agtatcaaat
    17821 ctcaatttag ctggcttgat gttctctgcg aattccttta agcctgtaat agagtctttc
    17881 gcttttttca agctgtcttt atctacacta aatgtaactg tgttcttaat tttagaagtg
    17941 ttaatttcta acatttaaaa tccccttatc aatcattttt accaccttct ttacttaaga
    18001 aatggtgtag agcctgatca atactctcgt ttaatc
//
LOCUS       DAPGWD010000082        18030 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPGWD010000082 DAPGWD010000000
VERSION     DAPGWD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.082"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1395
                     /gene="tnaA"
                     /locus_tag="RHX88_002736"
     CDS             <1..1395
                     /gene="tnaA"
                     /locus_tag="RHX88_002736"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDV2231405.1"
                     /translation="PEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDVFIDLL
                     TDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRGAEQIY
                     IPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDF
                     KGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVV
                     MDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMK
                     DDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYL
                     VDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSF
                     LLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYE
                     PKVLRHFTAKLKEV"
     gene            1486..2733
                     /gene="tnaB"
                     /locus_tag="RHX88_002737"
     CDS             1486..2733
                     /gene="tnaB"
                     /locus_tag="RHX88_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDV2231406.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLVFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2866..4041
                     /gene="mdtL"
                     /locus_tag="RHX88_002738"
     CDS             2866..4041
                     /gene="mdtL"
                     /locus_tag="RHX88_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDV2231407.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGVALLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4016..4975
                     /gene="yidZ"
                     /locus_tag="RHX88_002739"
     CDS             4016..4975
                     /gene="yidZ"
                     /locus_tag="RHX88_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDV2231408.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELRRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5132..5881
                     /locus_tag="RHX88_002740"
     CDS             5132..5881
                     /locus_tag="RHX88_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDV2231409.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5903..6469
                     /gene="yieF"
                     /locus_tag="RHX88_002741"
                     /gene_synonym="chrR"
     CDS             5903..6469
                     /gene="yieF"
                     /locus_tag="RHX88_002741"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDV2231410.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6523..7860)
                     /gene="adeP"
                     /locus_tag="RHX88_002742"
     CDS             complement(6523..7860)
                     /gene="adeP"
                     /locus_tag="RHX88_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDV2231411.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8027..8692
                     /gene="yieH"
                     /locus_tag="RHX88_002743"
     CDS             8027..8692
                     /gene="yieH"
                     /locus_tag="RHX88_002743"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDV2231412.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8759..9232
                     /locus_tag="RHX88_002744"
     CDS             8759..9232
                     /locus_tag="RHX88_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HDV2231413.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9281..9868
                     /locus_tag="RHX88_002745"
     CDS             9281..9868
                     /locus_tag="RHX88_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDV2231414.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(9930..10652)
                     /locus_tag="RHX88_002746"
     CDS             complement(9930..10652)
                     /locus_tag="RHX88_002746"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV2231415.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10667..11836)
                     /locus_tag="RHX88_002747"
     CDS             complement(10667..11836)
                     /locus_tag="RHX88_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDV2231416.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGGPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11863..13479)
                     /gene="bglH"
                     /locus_tag="RHX88_002748"
     CDS             complement(11863..13479)
                     /gene="bglH"
                     /locus_tag="RHX88_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDV2231417.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13565..14959)
                     /gene="bglB"
                     /locus_tag="RHX88_002749"
     CDS             complement(13565..14959)
                     /gene="bglB"
                     /locus_tag="RHX88_002749"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDV2231418.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(14978..16855)
                     /gene="bglF"
                     /locus_tag="RHX88_002750"
     CDS             complement(14978..16855)
                     /gene="bglF"
                     /locus_tag="RHX88_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDV2231419.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(16989..17825)
                     /gene="bglG"
                     /locus_tag="RHX88_002751"
     CDS             complement(16989..17825)
                     /gene="bglG"
                     /locus_tag="RHX88_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HDV2231420.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4522 a         4515 c         4280 g         4713 t
ORIGIN      
        1 cctgaaccgt tccgcattcg tgttattgag ccagtaaaac gtaccactcg cgcttatcgt
       61 gaagaggcaa ttattaaatc cggtatgaac ccattcctgc tggatagcga agatgtgttt
      121 atcgatttac tgaccgacag cggcaccggg gcggtgacgc agagtatgca ggccgcgatg
      181 atgcgcggcg acgaagccta cagcggcagc cgcagctact atgcgttagc cgagtcagtg
      241 aaaaatatct ttggttatca atacaccatt ccgactcacc agggccgtgg cgcagagcaa
      301 atttatattc cggtactgat taaaaaacgc gagcaggaaa aaggcctgga tcgcagcaaa
      361 atggtggcat tctctaacta tttctttgat accacgcagg gccatagcca gattaacggc
      421 tgtaccgtgc gtaacgtcta tatcaaagaa gccttcgata ctggcgtgcg ttacgacttt
      481 aaaggtaact ttgaccttga gggattagaa cgcggtattg aagaagttgg cccgaataac
      541 gtgccgtata tcgttgcgac catcaccagt aactctgcag gtggtcagcc ggtttcactg
      601 gcaaacttaa aagcgatgta cagcatcgcg aagaaatacg atattccggt ggtaatggac
      661 tccgctcgct ttgccgaaaa cgcctatttt atcaagcagc gtgaagcaga atataaagac
      721 tggaccatcg agcagatcac ccgcgaaacc tacaaatatg ccgatatgct ggcgatgtcc
      781 gccaagaaag atgcgatggt gccgatgggc ggcctgctgt gcatgaaaga cgacagcttc
      841 tttgatgtgt acaccgagtg cagaaccctt tgcgtagtgc aggaaggctt cccgacatat
      901 ggcggcctgg aaggcggcgc gatggagcgt ctggccgtag gtctgtatga cggcatgaat
      961 ctggactggc tggcttatcg tatcgcgcag gtgcagtatc tggtcgatgg tctggaagag
     1021 attggcgttg tctgccagca ggcgggcggt cacgcggcat tcgttgatgc cggtaaactg
     1081 ctgccgcata tcccggcaga ccagttcccg gcacaggcgc tggcctgcga gttgtataaa
     1141 gtcgccggta tccgtgcggt agaaattggc tctttcctgt taggccgcga tccgaaaacc
     1201 ggtaaacaac tgccatgccc ggctgaactg ctgcgtttaa ccattccgcg tgcaacatat
     1261 actcaaacac atatggactt cattattgaa gccttcaaac atgtgaaaga gaatgcggcg
     1321 aatattaaag ggttaacctt tacctacgaa ccgaaagtat tgcgtcactt caccgcaaaa
     1381 ctgaaagaag tttaattaat actacagagt ggctataagg atgttagcca ctctcttacc
     1441 ctacatcctc aataacaaaa atagccttcc tctaaaggtg gcatcatgac tgatcaagct
     1501 gaaaaaaagc actctgcatt ttggggtgtt atggttatag caggtacagt aattggtgga
     1561 ggtatgtttg ctttacctgt tgatcttgcc ggtgcctggt ttttctgggg tgcctttatc
     1621 cttatcattg cctggttttc aatgcttcat tccgggttat tgttattaga agcaaattta
     1681 aattatcccg tcggctccag ttttaacacc atcaccaaag atttaatcgg taacacctgg
     1741 aacattatca gcggtattac cgttgccttc gttctctata tcctcactta tgcctatatc
     1801 tctgctaatg gtgcgatcat tagtgaaacg atatcaatga atttgggtta tcatgctaat
     1861 ccacgtattg tcgggatctg cacagccatt ttcgttgcca gcgtattgtg gataagctcg
     1921 ttagccgcca gtcgtattac ctcattgttc ctcgggctga agattatctc ctttgtgatt
     1981 gtgtttggtt ctttcttctt ccaggtcgat tactccattc tgcgcgacgc caccagttcc
     2041 actgcgggaa cgtcttactt cccgtatatc tttatggctt tgccggtgtg tctggcgtca
     2101 tttggtttcc acggcaatat tcccagcctg attatttgct atggcaaacg caaagataag
     2161 ctaatcaaaa gcgtggtctt cggttcgctg ctggcgctgg tgatttatct cttctggctc
     2221 tattgcacga tggggaatat tccgcgcgaa agctttaagg cgataatctc ctcaggcggc
     2281 aacgttgatt cgctggtgaa atcgttcctc ggcaccaaac agcacggcat catcgagttt
     2341 tgcctgctgg tgttctctaa cttagctgtc gccagctcgt tttttggtgt cacgctgggg
     2401 ttgttcgatt atctggcgga tctgtttaag attgataact cccacggcgg gcgtttcaaa
     2461 accgtgctgt taaccttcct gccacccgcg ttgttgtatc tcgtcttccc gaatggcttt
     2521 atttacggga tcggcggtgc cggactgtgt gccaccatct gggcggtcat tatccccgcg
     2581 gtgctggcaa tcaaagctcg caagaagttt cccaatcaga tgttcacggt ctggggcggc
     2641 aatcttattc cggcgattgt cattctcttt ggcataactg taattttatg ctggttcggt
     2701 aacgttttta acgtgttacc taaatttggc tgatcccttc aagaagccag ctattttgct
     2761 ggcttcttgc ctgtcaggaa atctcttatg tccaaatggc aactcgcctg attctccttc
     2821 accacgtatg ctttgcgtca ccttactatc aggacgcttt agcccatgtc ccgcttttta
     2881 atttgtagtt ttgccctggt tttactttat cccgccggga ttgatatgta cctcgttggc
     2941 ttaccgcgca tcgccgccga tctcaatgcc agcgaagcgc agttgcatat tgcgttctct
     3001 gtttatctgg cgggcatggc agctgcgatg ctatttgccg gtaaagtggc cgatcgttca
     3061 gggagaaaac cggtcgccat ccccggcgcg gcgctattta ttatcgcttc ggtgttctgt
     3121 tcactggctg aaaccagcac gttatttctt gcaggccgct ttctacaggg gcttggcgcg
     3181 ggctgttgtt acgtggtggc gttcgccatt ttgcgcgaca cgctggatga tcgacgtcgg
     3241 gcaaaagtgc tgtcattact caacggtatt acctgcatca ttccggtgtt agcgccggtg
     3301 ctcggacatc tgattatgct taaattcccg tggcagagtc tgttctgggc gatggcaatg
     3361 atgggtgtcg cgttactgat gttgtctttg tttattttaa aagaaacgcg ccctgcggcc
     3421 cccacaactt cggacaaacc acgagaaaat agcgagtcgc tgcttaatcg gtttttcctc
     3481 agccgtgttg ttatcaccac cctcagcgtt tcggtgatcc tcaccttcgt caacacgtcg
     3541 ccagtattgc tgatggaaat catgggtttt gagcgcggag aatacgccac cattatggcg
     3601 ttgaccgctg gcgtcagcat gaccgtttca ttctccacgc catttgcgct gggaattttt
     3661 aagccacgta cgttgatgat cacctcgcag gtgttattcc tggcggcggg gatcaccctt
     3721 gctgtttcac cttcccatgc ggtttctctg tttggtatca cactgatttg cgccggtttc
     3781 tcggtcggtt ttggcgtagc gatgagtcag gcgttagggc cgttttcatt acgcgcgggc
     3841 gtagccagct cgaccttagg tattgcgcag gtttgcggtt cgtcactgtg gatttggctg
     3901 gcggcggtgg ttggtatcgg cgcatggaat atgctgatcg ggattctgat tgcctgtagc
     3961 atagtaagcc tgttgctgat tatgttcgtc gcgcctggac gccccgttgc cgctcatgaa
     4021 gaaatccatc accacgcttg atctcaatct gctgctctgt ctgcaactgc tgatgcagga
     4081 gcgcagcgtg accaaagcgg cgaagcggat gaacgtgacc ccttcagcgg tgagtaagtc
     4141 gctggcaaag ttaagagcgt ggtttgacga cccgctcttt gtgaactcac cgctgggcct
     4201 gtcgcccaca ccgctaatgg tcagcatgga gcaaaatctg gcggaatgga tgcaaatgag
     4261 caatctgctg ctggataaac cgcaccacca gacaccgcgc ggcctgaagt ttgagttggc
     4321 ggcggaatcg ccgctgatga tgatcatgct taatgcgctg tcgaaacgga tctaccaacg
     4381 ttacccgcag gcgaccatca aactacgtaa ctgggattac gattccttag atgccattac
     4441 tcgtggtgaa gtggatattg gtttttccgg tcgcgaaagc catcctcgct cgcgggagct
     4501 gttaagctcg ctaccgttag ccattgatta tgaagtgctg tttagtgatg taccctgcgt
     4561 ctggttacgc aaagatcatc cggcactgca tgaaacgtgg aatctggaca cctttttacg
     4621 ttatccgcat atcagcattt gctgggaaca gagcgacacc tgggcgctgg acaatgtgtt
     4681 acaggagctg aggcgcgaac gcacgattgc tatgagcctg ccggaatttg agcagtcact
     4741 gtttatggca gcgcaacccg acaatctgct actggcgacc gcgccgcgct actgtcagta
     4801 ctacaatcaa ctccatcaac tgccgttggt tgctcttcct ctcccgtttg acgaaagcca
     4861 gcaaaaaaag ctggaagttc cttttaccct gctgtggcat aaacggaaca gccataatcc
     4921 gaagatcgtc tggttacggg aaaccattaa aaacctttac gcgtcgatgg cataaccgaa
     4981 tcgtatgaaa cgcgacccaa tttcacaata aaatgtaaaa aagttgtaat aagcttgtct
     5041 gaatcgaact tttagccgct ttagtctgtc catcattcca gtaaatgatt actcttgtat
     5101 tcataatgga ccattaagca tggagcgaaa aatggcgact cactttgccc gagggatttt
     5161 aacggaagga catctgattt ctgttcgtct cccctcccag tgtcatcaag aagcccgaaa
     5221 cattccccct catcgtcaaa gccgttttct ggcgtctaga ggtttactcg cagaactgat
     5281 gttcatgctg tatggcattg gcgaattgcc ggaaatcgtc accctgccga aaggtaaacc
     5341 ggttttcagt gataaaaatt tgccttcgtt ttctatttcc tatgccggga atatggttgg
     5401 cgtggcgtta acaaccgaag gtgaatgtgg tctcgatatg gaactacagc gtgcgacgcg
     5461 cgggtttcat agcccacacg cgcccgataa ccacaccttt tccagcaatg aatcgctatg
     5521 gatcagtaaa caaaacgatc ctaacgaagc gcgggcgcag ctcatcacgc tgcgccgaag
     5581 cgtgctaaaa ctaaccggtg atgttttgaa tgacgatccg cgcgatctgc agctgctgcc
     5641 cattgccgga cgcctgaaat gtgctcatgt aaatcatgta gaagcgttat gcgacgcgga
     5701 ggacgtgctg gtgtggtccg tggcggtcac gcccacgatt gaaaagctca gtgtctggga
     5761 gttcgatggc aaacacggct ggaaaagcct gccggatatt cacagccgcg ccaacaatcc
     5821 taccagccgg atgatgcgtt ttgcccagct ctctaccgtg aaggcttttt cgccaaattg
     5881 atagacaacc acaggagtca tcatgtctga aaaattgcag gtggttacgt tactggggag
     5941 cctgcgcaaa ggctcattta atggcatggt tgcacgtacc ctgccgaaaa ttgctccggc
     6001 gagcatggaa gtcaatgcgt taccttccat tgccgacatt cccttgtatg acgctgacgt
     6061 acagcaggaa gaaggttttc cagcaacggt tgaagctctg gcggaacaga tccgtcaggc
     6121 tgacggtgtg gtgatcgtca cgccggaata taactactcg gtaccgggtg ggctgaaaaa
     6181 tgccatcgac tggctttccc gcctgccgga tcaaccgctg gccggtaaac cggtgttgat
     6241 tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt cagtatcacc tgcgccagat
     6301 tctggttttc ctcgatgcaa tggtgatgaa caagccggaa tttatgggcg gcgtgattca
     6361 gaacaaagtt gatccgcaaa ccggagaagt gattgatcag ggtacgctgg accacctgac
     6421 cgggcaattg accgcatttg gtgagtttat tcagcgagtt aagatctaaa taaaaaaccc
     6481 gccagcaatc atgcatggcg ggtttttaac gcgctatcga ttttagtgag cgtcgataaa
     6541 gacaatcttc aggataaaca gcagcgcaac gatgattacg cacgggctta gatcacgcag
     6601 acgtccggta ccgattttca tcacgcagta ggagataaag cccagcgcga taccttcggt
     6661 aatcgagaag ctgaacggca tcatcacggc ggtaataaac gccggaacag attcagtaag
     6721 atcctgccag ttcactcgtg ccagactgga agtcatcagc acgccaacgt aaatcagcgc
     6781 accagctgca gcgtagcctg gcaccatccc cgccagcggc gacagaaaga taaccagcag
     6841 gaacagcaga ccaacaacca ctgccgtcag accggtacga ccgccaaccg atacgccgga
     6901 agaggactca atataagccg taacggaaga agtaccgata aacgaaccgg tcacggaaga
     6961 gatactgtcg acatacagcg cctgcttcat gcgcgggaat ttacccttct catccgccag
     7021 gcctgcttta tcggtcacgc caatcagcgt accggaggag tcaaacaggt tgaccagcat
     7081 gaaagagaat atcacccctg ccagcccgag gttaaacgac ccggctaaat ctacatggcc
     7141 cacgacagtc attacgctcg gcggcgcaga aacgatgcca ttgtagtgga catcacccag
     7201 catccagccc agcagcgttg tcaccacgat ggaaaccagc accgccgcgt gaatgttgcg
     7261 cgaggccaga atagcgatga tgaagaagcc gaggatcccc agcagcacgc tgtgagaagt
     7321 caggttaccg atgctgacca gcgtttccgg gttagcgaca atcacacctg cattcttcag
     7381 tcccatcata ccaatgaaca gaccgatacc gctggtgatg cccacacgca gacttaccgg
     7441 aatgttagca atcatccagt aacgaacgcg gaaaatcgtc agtaacagca gaccaatcgc
     7501 gccccagaag attgcgccca tcccgacttg ccacggcaag cccatagcct gtacaacgac
     7561 aaaagcgaag aatgcattca ggcccatagc gggtgccagt gcaactggca ggttagcaaa
     7621 cagtcccatc ataatactgc cgaatgcagc aatcagacag gtagtaacga agacggcgct
     7681 ggtatccatg ccagcaacgc caagaatttg cgggttaaca aaaacgatgt aaaccatcgt
     7741 caggaaggtg gtaaaaccgg cgatcacttc ggtccgtgcc gtcgtgccat gttcgcgcag
     7801 tttaaacacg cgttccagca tcccctggcc agaagcctgg gttgtgtgtt gttgactcat
     7861 tatctatttc cgaacaagga gggaaaatcc gtcgctatcg tataccaaaa tgagacaata
     7921 tggacggctg aagataactt tttttcattg tatttgctta tacggcaacg attgcgtcat
     7981 gcgaattggc atgacgaaaa cgttaaacta acgataaagg aaagacatgt cccagataga
     8041 agcggtattt ttcgactgcg acggtacgct ggtcgacagt gaagtcattt gctcccgcgc
     8101 atatgtaacg atgtttcagg aatttggtat tacgctcgat cctgaagagg tattcaaacg
     8161 tttcaaaggt gtaaaactgt acgaaattat cgatattgtt tcccttgaac atggtgttac
     8221 gttagcgaaa acagaagctg aacacgttta ccgtgcagaa gtcgctcggc tgttcgattc
     8281 agaactggaa gccatcgaag gggctggagc gctcctgtca gcgatcactg cgccaatgtg
     8341 tgtggtatct aacggcccaa ataacaaaat gcagcattct atgggcaagc tgaatatgtt
     8401 gcactacttc ccggataaac tgttcagcgg ctacgatatt cagcgctgga agccagaccc
     8461 ggcgttaatg ttccatgcgg caaaagcgat gaatgtaaat gtagaaaact gcattctggt
     8521 tgatgactca gttgccggtg cgcaatctgg tatcgacgca ggtatggaag tgttctactt
     8581 ctgcgccgac ccgcacaata agccgatcgt tcacccgaaa gtcaccacct ttacccatct
     8641 ttcgcagtta ccggaactgt ggaaagcgcg tggttgggat attacggcat agttcttcac
     8701 actcccttca cttaccccgc ttaaattggc gctcaaaggt aagtaaaggg tgtttgatat
     8761 gtctgtttca cgtcgggtaa tacatcacgg actttatttt gcggttttag gaccgttaat
     8821 tggtgttctg tttcttgtcc tctacatatt cttcgcaaaa gaaccgctgg ttcttttggt
     8881 aataatacag gtacttccat tgtttctctt attgtcgatt actacgggag ctattcctgc
     8941 attgttaacc ggcgtgatgg tcgcctgtct gccggaaaag atcgggtcac agaaaaatta
     9001 tcgttgtctg gctggtggca taggtggcgt cgttatcacc gagatctatt gtgcagttat
     9061 tgtacatatt aagggcatgg cttcctcgga gttgtttgaa aacattcttt ctggtgacag
     9121 tctcgttgtc cgcatcattc ctgcattgct ggcaggtgtg gtgatgagca gaatcattac
     9181 ccgtctgccc ggattggata tttcatgtcc tgaaacagac tctttaagtt aagcgggata
     9241 ctttatcttt gggctactca aaagcagaca ggatgttact atgactcaaa atatcaggcc
     9301 gttaccccaa ttcaaatatc atcccaagcc actggaaaca ggcgcatttg aacaggataa
     9361 aaccgtagag tgcgattgct gtgaacaaca gacgtcagtt tattactcgg gtccctttta
     9421 ttgcgttgat gaagttgaac atctctgtcc gtggtgtatt gcggacggtt ctgctgctga
     9481 aaagtttgca ggtagttttc aggatgatgc cagcatagaa ggtgttgaat ttgagtatga
     9541 tgaagaggac gaatttgccg gtattaagaa cacatatcct gatgaaatgc tgaaagagtt
     9601 ggttgaacgc acgccaggtt atcatggatg gcaacaggaa ttctggcttg cgcattgtgg
     9661 cgatttctgt gcttttatcg gctatgtggg ctggaatgat ataaaagatc gcctcgatga
     9721 atttgccaac cttgaagaag attgtgagaa tttcggtatt agaaattctg atctagctaa
     9781 atgcctgcaa aagggtggtg attgtcaggg ttatctcttc cgctgtctcc actgcggcaa
     9841 gctgagactg tggggtgatt tttcgtagtt atttaaataa tgagaacagg ccggagcgta
     9901 attcatacat ccggccttat ttcttcagct taaccgagcg ctaattctgc ggcagcggct
     9961 ttatcagcga ttaccatcag cgaaggatgc agctgcaaca cggaagctgg aacatcttcg
    10021 gtaaccggac cttgcagcac atttttcagt gcctgcgctt tacctgcgcc actgacaatt
    10081 atcagcaagt ttttcgccgc catgatgctt ttcggcccca tcgtcacgta gctgtcaggc
    10141 actaacgaaa agtcgccgcc caactcacca tgcgccacaa tatcaaccat ctctccctgg
    10201 atcgggaact ccacggtttg ctcatggaaa tgggtcgtat tcggcaaatt gccacaaaaa
    10261 tgaccatctg cacctaatcc cagcaccacc aaatccaggc cgccttcacg cgccagtttc
    10321 tgatcatgct cgcggtagtt atcaatagtg agcttctgga tattctcttc tttgatcccc
    10381 gcaggggtga aaaacagatt acgcagattg gtaatcgtta caccttctcc ctctttgccg
    10441 cgaaagggaa tttcatcgaa attatagaaa tagcagttat cgtaccaggg cttaccttta
    10501 accagggtgg tgaggtattc atacatgcct ttgggcgtgc taccggcggt aattgccagg
    10561 ttaacacgac gcgtcttcga catataacca agcagatggt gtgccgcgac acggctcatt
    10621 tcctggtaat cttcggtaat gattaatttc atcttagtgc cttattttat ttaaataatt
    10681 tctgtacgaa ggctgcatag gcaggccgcc agacatccat ttcatggttc aggccgggat
    10741 attcctggta atcaaactta atttttttct gctcaagctc agttttcagc ccggcgatat
    10801 ccttgccggt tacgacatct ttatcaccaa cgaccacagt aaaattacgt agttgctggt
    10861 taatagctgc cggatcgttc agccgggccg cgacaccttc atccggtacg gttgtcgtgg
    10921 taacaccact gaatgtggcc agccagccaa agctttccag atgattcatt ccggaaacca
    10981 gcgcctggta cccgccttgt gaaagccctg ccagcgcgcg accatcggca tctttacgga
    11041 cattaaaacg cttgctaatc agcgggataa tatcgttcat cagttcgcga tctgccgctt
    11101 tagcattcag cggataaaag actttacgtc tttcctgagg cacgaaatct tcgggaataa
    11161 tgcccttcgc atcggtttcg gtatccggga tcaccaccag catcggttta attttccctt
    11221 cagccagcag gttatccatg atttgcggga tacgcccctg atcgatagcg gaacgtccgg
    11281 tatcaccaaa accgtgatag aaatagagca ctggcaaagg ctcgcccatg ccggtgtatc
    11341 ctggcggggt ccagacatac atctgacgtt cagattgcaa tgcgttggag tggtaagtta
    11401 tggcgatcaa atcaccatgc gcaacagaac gcgtatccag ataactgcct ggcaccagaa
    11461 tcatactgga gttaacctgg cgctgaggct tagtcattgc ggtgcctgta tcaatgctgc
    11521 gtacaccatc aacattgaaa aaatactcgt acaggttgcc tttcaggata ggtgtgcgcc
    11581 acgaccagac tcctgcttcg tctttggtca tcgggtgaat attgtccgga accggaccac
    11641 ccaccactac agagacattt tttgcgccag gggcaaagta gcgaaaggtc acgctgttat
    11701 cggcattgac ctgagtcaca tactgtttca ccgggatagt gggtgccggg ctggctggca
    11761 tatccgctgc tatggcccac tgagcagaaa taccgctggc aatagccagc gttaaagcag
    11821 caatttttat attcatcgta atattccttt cattaagttc cattaccacc agatttcagc
    11881 ctgggcaccg acagcaaact ggtcgtcttt attgtcttcg aaggtgaagc catccagttc
    11941 gttttccagg gctttgatat acgtggcgta gaaacgaatt tccggacgcg aggtcaacat
    12001 actggtattg actttaaagg tatggaagag cgtggtttta taaccagact cattaaattt
    12061 attactattc gcatctttgt tttgctgggt gaaatagccc agttcaacac ctgtctggtt
    12121 atattggtcc caaatatagg ccgggcgaac aaccgcacga atagattcga aatcagagtg
    12181 tgcgcctgtt tcgtagctat aaatatcgtt accgaaggag taaacaatcg cgttagctac
    12241 aataaaatga tcgccgatat aggcttcgcc ctgcgaagtc agacgaactg ctgttccacc
    12301 agtgtgatca ccataataac gaccattaaa ggtggtaaat ggacttgcgc cagcataacg
    12361 gccaaagtta ctggcgatag aattattcgc gaccaggaag gagaattcgt tgaagccacc
    12421 tttatcaaat ttctgcgtta aagatgtgcc aaacatccag gtatctttcc agtcataata
    12481 cccgttatta tcctgattat ctttttccga tgcgctttcg tttgccgtga cataacgacc
    12541 acttaccatt aaggtggctt tatcccataa cgggatatct ttatagcgta aatcaatggt
    12601 attggtatta atctgctgtt tgttttgcag gctgcgatcg taatcatcga catcttcgcg
    12661 aaccagcgcg atatcaattt tacccggacc gactttccag ttttccagac ctacacccgc
    12721 tgcggcatca gtacgctgcg ttttccagtc aagcatctgg atttcaattt tcggcgcacc
    12781 gtgtttaccc acccagaaat cagcctctgg cgcaaagggc aggaaacctt tggtggtaac
    12841 gtacatatcg gagaactgca taaagttctc gccaccggcg ttatcgccaa accagccggt
    12901 agagtactgc tgaccaacgt taccatccat catcacaacg gcatcaaccc gtttgccgtt
    12961 ttcgttgtag acacgttgtt ttaactgcaa atcaaaccag ccggagtatt cgttaccaaa
    13021 gcggcccaga gaaccaatcg cccatgattt aggtgaacca tgagaggcgg tcccccaacc
    13081 agaacggtaa tagccgttgt aactaaagcc aatttcatct ttcacaaact tactgaaatc
    13141 tttcagagtc atggaagaat aaatcgagcc tgtctcactg gcatttttct cttcattttt
    13201 gaccagtaca gcatcaggtt ttgcggcact actggtcggg aacggattgg cggcataccc
    13261 ttgatcattc gtacttacgc tacgattcac cgtcgctggc gtatattttt tcttctcttc
    13321 atctttatac tttttgagtt cgctttgcgt ttctcttaac gctttttcta ataactcaag
    13381 gcgttgttcc accgttaatg attcagccaa tgattgtgca gaaaaggcta acggtgccat
    13441 taataagatg gcagaggtaa taagatttcg tctaaacata atatcccttt atggtgcaaa
    13501 gaaagaatta acgtatcgca tccagactgt tctgaatgcg acgataatta aggtgcttta
    13561 ttggttattt ttttaatgac agcccccgcg ttttgatcac ttctgcatac catccgaagc
    13621 ttttcttacg cgttcttgtg aggcttcctt cgccattatc atcacgatcc acataaataa
    13681 agccgtagcg cttagacatt tgtgaatgag atgcactgac taaatcaatt ggcccccaac
    13741 tggtgtaccc cataatatcc acaccatcgg caatcgcttc atttacctgt accaggtgat
    13801 cgtttaaata ggcaattcga taatcgtcct gtatcgaacc atccgcttca acgctgtctt
    13861 ttgcgcctaa tccgttctcg acaataaata acggtttttg ataacgatcc caaagcgtat
    13921 ttaacagaac ccgtaatcca accggatcaa tttgccaccc ccactctgaa cttttcagat
    13981 gcggattggg gatcatattc agtatgttgc cctgcgcatt tttattaatg ctttcgtcgt
    14041 gggaaacaca accagtcatg taataactaa aagagatgaa atctacagta tgttttaaat
    14101 cttctgcgtc actttcagtc atctcaatgg tgatattgtg gtcgcggaag aaacgctgca
    14161 tatagccggg atactggcca cgcgcctgaa catcaccaaa gaacatccag cgccggttct
    14221 cttccatggc ctgcaacata tcctgtggct ggcaggtgag ggggtaaacc agcccaccga
    14281 gaagcatatt gccgattttc gcttcgggga gcaggctatg acaggcttta actgcccgcg
    14341 cactggcaac cagttgatgg tggatagcct gataaacttc cgcctcgcca ctctcttctg
    14401 ccagccccac gcccgtgaat ggctcgtgta acgacatgtt gatttcatta aacgtcagcc
    14461 ataacgccac tttatgttgg tagcgagtaa agaccgtgcg ggcgtaatgc tcgaagtgat
    14521 cgatgaccgc tcgattagcc caaccgccgt agtttttcac cagcccatat ggcatttcgt
    14581 aatgggataa cgttaccagc ggcttgatcc ccgcctgcgc catttcatca aacagccgat
    14641 cgtaaaacgc taaccccgct tcattcggtt cgacttcgtc gccctgaggg aaaattcgcg
    14701 cccaggcaat ggaaatacgc agacaggtga agcccatctc ggcaaataac gcgatatctt
    14761 ccgggtaacg gtgataaaaa tcgatggcga catctttgat attctctttc cccaggatgc
    14821 gcggttccat ttttcccatt acgccatgag gctgtaaatc tgaggtcgag atccctttgc
    14881 catcttcctg ccaggcacct tccacctgat tggcagctgt tgcgccaccc caaagaaatg
    14941 tttctggaaa tgctttcata attaactcct tttatcgtta gcgaatgatg gataacagcg
    15001 gttcacctgc gcttatctgc gccgtgccgt ggggtaatac gtccgtaaaa tcatcgctat
    15061 tactgattaa taccggcgtc gtcagatcaa atccggcctc gcgaatagca gggatatcaa
    15121 aagaaatcag ccgatcgcct gtattgacct tgtcacccac gttgacgtga gcggaaaaga
    15181 atttgccgtc cagttttacg gtgtcgatac cgacatgaat caggagctcc acaccatcat
    15241 ctgactcaat gccaatggcg tgtaatgtgg cgaacaacga agcaattcga cccgcaaccg
    15301 gagaacgcac ttcaccaacc gagggcagaa tggcgatacc tttacccaac aggccactgg
    15361 caaacgtggt atcagcgacg tgaatgagcg gcacaatctc tcccgtcatc ggtgaacaga
    15421 taccgccctg ctcaggtggt gtaataacct ctggtgtttt ctcttgcggg gcaccctgcg
    15481 ctggctgacg tttagcggtg atgaaatgaa gcatcaccgt accgacaaat gcgcaaccga
    15541 tggcaatgac accgccaata acgctggccc agacggtgaa atcaattccc gttgacggga
    15601 tggtttgcat gaaggtgaaa atacttggca aaccaaagga gtacactttc gtttgcgcgt
    15661 agccaatgat ggtggccccc aaagccccac tgatacaggc gataacaaag gggtacttac
    15721 gcggcagatt aacgccatat accgctggtt cggtgatacc aaacaggccc gtcaacgccg
    15781 ctgatcccgc caccactttt ttctgcgcat cgcgttcgca gaggaagacg ccgagcgccg
    15841 ccccgacctg cgccataatg gcgggcatta acagcgggat catggtgtcg tagcccagca
    15901 cggtgaagtt attgatacac agcggcacca ggccccagtg cagtccgaac atgacgaaga
    15961 tttgccagaa gccgcccatt accgcgcctg caaatgcagg aaccgcctga taaagccaga
    16021 gataaccggc ggcaatcagt tcgcttatcc aggttgatag cggccccacc agcagaaagg
    16081 tgacgggtgt gataaccatc agacatagca atggtgtgaa gaaatttttg attgccgacg
    16141 gtaaccacgc attaagtcgg cgttccagaa tgctgcacaa ccaggcagaa aaaataatgg
    16201 gaataaccga tgacgagtaa ttcaacaatg tgaccggaat acccaggaaa tccagcccca
    16261 gctcatccgc tttttgcccg ttctcgaaag cagtcagaat taatggatgc actaacgctc
    16321 caccaatcac catggcagta aatggattac cgccgaagcg tttccccgcg gtgtatccca
    16381 ggattatcgg gaagaaccaa aacaaggcat cactggcgct gaataaaatt aaataagtac
    16441 cactttgttc ggtcgtccac tgaaaagtga gcgccagagc cagcatacct ttcaggatcc
    16501 cggttgccgc catcaaaccg atcagaggcg taaaaatacc tgaaataaca taaacaaagc
    16561 ggtttagcag attacctttc tcatcatttt ccggtgcctg ttgcgctttt tcgccaaggc
    16621 ctgccacgct gttgaccgcc aggaagacat cggccacatg gttacctatg accacctgaa
    16681 actgaccacc gctttccacc accataataa taccgggggt ctttttcagt acctcagctt
    16741 gcgctttgct ttcatccttt aacttaaaac gtaatcgcgt tgcgcaatgc atcagactca
    16801 caatgttatc tgcgcccccg actcctgcga ctatttttct ggctaactcc gtcataactt
    16861 gccctctacc gctttgcggc aaaactccaa aaaaaaacct gaaaaaaacg gcctgacgtg
    16921 aatcaagcaa tttttttcag gttttgcccg cttagtgcgg taacaatcct ttactcagta
    16981 ataatatttc agtgttcttt gcgcacgcgc tctatattta tggctaaaaa cataatctct
    17041 gcgggtgaaa ttttacgttg atactgcaaa ccaataaaaa tggcgatccg ttccgcacat
    17101 tgccatgctt gcgggtaatt ttgttttact gcttgttgta atgattcatc actatcgtta
    17161 attgaagcat gttcaagaat acgccaggat aaaaacttca gatgtgtaac cagtcgctga
    17221 taactcaagc tttcttcctg gtaattaagg ctgaactgaa attttattaa ttgcagcatt
    17281 tcgcgcatta actgcgtgac acctgcaaca tcctccatat ttccgctcat ttgggcactg
    17341 accagatgca tggcaataaa gcccacttca tctttcggta actgcacgcc caaccgttta
    17401 tcaataatgg ttaacgcttc ttcccctagc tggaactctt tcgggtaaag ccgctggata
    17461 tcccacagca acgggttggg cagcaacacg ttttgctgaa agcgtttaat cgcaaactgg
    17521 caatggtcag ttagcgagat ataaatactg tcctgtaatt ttcccaagcg ctcctgcgct
    17581 aaagagataa tacgatcaca ggttgccatc acctcaagag gaatatgact taagagttcg
    17641 cttaatcgcc cgttcagttc atgactgctc aaggcatact ctttttctat tccacttgag
    17701 ttaattcttt cgccagcgcg tttttgaaag ccaattcctc gccccatgac gactttttcc
    17761 cgctgttgat catcaataac caccacaaca ttattgttga gaattttggt gatttgcatg
    17821 ttcatagcaa ggaccttttt tataaacaaa aaaacccgac ttcaccagta ttctctggtt
    17881 atgtcaggtt ttgcctgcga atgcagtaac aatccagtca tttattaatg gtttttataa
    17941 cgaacatcca ggttcggaaa ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt
    18001 gataaaaata tgaccatgct cgcagttatt
//
LOCUS       DAPGWD010000083        17734 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPGWD010000083 DAPGWD010000000
VERSION     DAPGWD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.083"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..707
                     /locus_tag="RHX88_002752"
     CDS             <1..707
                     /locus_tag="RHX88_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV2231421.1"
                     /translation="NKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSW
                     GINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVA
                     QTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMHY
                     FSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPL
                     TVQPECPVTLKDVSDL"
     gene            704..2155
                     /gene="djlC"
                     /locus_tag="RHX88_002753"
     CDS             704..2155
                     /gene="djlC"
                     /locus_tag="RHX88_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDV2231422.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2215..3885)
                     /gene="hscC"
                     /locus_tag="RHX88_002754"
     CDS             complement(2215..3885)
                     /gene="hscC"
                     /locus_tag="RHX88_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDV2231423.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(3969..4904)
                     /gene="rihA"
                     /locus_tag="RHX88_002755"
     CDS             complement(3969..4904)
                     /gene="rihA"
                     /locus_tag="RHX88_002755"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDV2231424.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5022..5747)
                     /gene="gltL"
                     /locus_tag="RHX88_002756"
     CDS             complement(5022..5747)
                     /gene="gltL"
                     /locus_tag="RHX88_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDV2231425.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5747..6421)
                     /gene="gltK"
                     /locus_tag="RHX88_002757"
     CDS             complement(5747..6421)
                     /gene="gltK"
                     /locus_tag="RHX88_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDV2231426.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6421..7161)
                     /gene="gltJ"
                     /locus_tag="RHX88_002758"
     CDS             complement(6421..7161)
                     /gene="gltJ"
                     /locus_tag="RHX88_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDV2231427.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7331..8239)
                     /gene="gltI"
                     /locus_tag="RHX88_002759"
     CDS             complement(7331..8239)
                     /gene="gltI"
                     /locus_tag="RHX88_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDV2231428.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8694..10571
                     /locus_tag="RHX88_002760"
     CDS             8694..10571
                     /locus_tag="RHX88_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDV2231429.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQKIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGEGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISIGDQQTSIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10649..12187)
                     /gene="lnt"
                     /locus_tag="RHX88_002761"
     CDS             complement(10649..12187)
                     /gene="lnt"
                     /locus_tag="RHX88_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDV2231430.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12212..13090)
                     /gene="corC"
                     /locus_tag="RHX88_002762"
     CDS             complement(12212..13090)
                     /gene="corC"
                     /locus_tag="RHX88_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDV2231431.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13180..13647)
                     /gene="ybeY"
                     /locus_tag="RHX88_002763"
     CDS             complement(13180..13647)
                     /gene="ybeY"
                     /locus_tag="RHX88_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDV2231432.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13644..14684)
                     /locus_tag="RHX88_002764"
     CDS             complement(13644..14684)
                     /locus_tag="RHX88_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDV2231433.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14837..16261)
                     /gene="miaB"
                     /locus_tag="RHX88_002765"
     CDS             complement(14837..16261)
                     /gene="miaB"
                     /locus_tag="RHX88_002765"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDV2231434.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16407..17582
                     /gene="ubiF"
                     /locus_tag="RHX88_002766"
     CDS             16407..17582
                     /gene="ubiF"
                     /locus_tag="RHX88_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDV2231435.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         4312 a         4448 c         4616 g         4358 t
ORIGIN      
        1 tgaataagga agaacaatat cttctttttg cgctttctgc gcccatggaa atacttaatc
       61 agggttgcaa acccgcgcat gactcgccaa aaatgtatac cggtataaaa gaattcgatc
      121 tctcatcatc ctggggaata aataatcgtg atgatttaat tcagactatt tatcagatga
      181 ctgatgatgg tcatgccaat gatttagctg gcttatatct gacatggcat cgctcttccc
      241 ctgaagagtg gaaagcatta attgctggtg gctcggagag agggttgatt tatacccaat
      301 tcgttgccca aaccgctatg tgctgtggag aagggggaat caaagcctgg gattatgttc
      361 gaatggggtt tctgtcacgg gtcggtgtgc tcaacaactg gctgacggaa gaagagagtt
      421 tatggctgca atcgtgcgtc tatgtacgag ctcaccatta ctatcacagc tggatgcact
      481 atttttccgc ctattcgtta ggacgtctct actggcaatc ttctcagtgc gaagacaaca
      541 cttcgctacg cgaggcgcta accctgtaca aatacgatag cgccgggagt cggatgttcg
      601 aagagctggc agcgggaagc gatcgtttct atgccacgct tccctggcag ccattaactg
      661 tccagcctga atgcccggta acgcttaagg atgtgagcga cctatgaaaa catgttggca
      721 aatcctcgaa attgaaagca cgacgcaaat agacattatc cgccaggctt atcttgctcg
      781 cttaccgtta tgtcatcccg aaaccgatcc gcaggggttt aaagcattac gccaggccta
      841 tgaagaggcg ctgcgactgg cggtaaatcc tgtcgaggaa gcagatgatg aagaaaaaga
      901 tgccgccgct gaacatgaaa tactgcgtgc attcaggaca ttactggatt cagaaagtga
      961 tcgttttcag ccctccgcct ggcagaaatt tattcagcaa ttaaatacct ggaacatgga
     1021 ggatgtcgat caattacgct ggccgctgtg tgcaatcgct atagaagcgc gatatctttc
     1081 attaaattgt gcttctttgc tggcagagcg tttgaactgg cattcgttta atgacagcga
     1141 aggaatggat gaggaagaaa gggaggcttt tcttgaggcc attcaggctg gtgattgttt
     1201 cgatttcctt agccttctgg aatatcccgt tgcgttgcag aaccagactg ttgagtatta
     1261 cttcgcgctg gaacgttgct gccgttacca tcctgactac gtcactgcgt ttttggcgat
     1321 ggaaggtccg tggttcattc ctgatgatgc aaagttacat cgcaaactgt tgcgctggta
     1381 cagctcggtg caaacaggta tggcggaact cattcctgta gccaaacagt ggcaaatgga
     1441 agaaccagaa agcgaagatg cccgatatta cttgtgtgct caaaggttgt actgcggcga
     1501 gggggaaagc ctgcttgccg atctctgcgc gtaccgggaa agttacccat ctacacaagc
     1561 tgataatttg ttgttgcagt ggagcaagcg tcattgcccg gattatttcg cgttattagt
     1621 gatggttatc gaagcgcgga gcatggtaga tgcgcaaggt caaccgctga aatatgttcc
     1681 tggtgagagc gcccggacgc ggctgttatg ggcggaaatt ttacatagcg gaaaattatc
     1741 gccgttaggt caatcgttta ttgagtcgtt attcttcaaa cgcaaagcat gggcgtggtg
     1801 gaaatcgaga gtcggtagcg agacagaaga agattcaccg ttactggatt tgtatcgggt
     1861 agcggaacag gtagtacttg aagcgtttcc gaaacaagag atgctggccc gtcttaatac
     1921 aaggctggaa ggcggagatg ctcatccatt agaggccatt atcacccgga tgctgttgac
     1981 gaaagtaaaa ctcgagccgg aggatgaaga tgtcgatgag ccaacacctg aaaaccatga
     2041 agaaaaaaat gatgagggtg aaaaaccaca gagcattacc agcattatca aaatcagttt
     2101 aacggtgctg gttataggtt atgctctcgg caaaatcgcg atgttgttta gttgacgatg
     2161 tgttttttgc gccgatgccg gatgcagcat ttacgccgca tccggcagcc gtgattatga
     2221 ggtctcaatg gctaaataaa cgctcgcccg ccgcctgacc tcatcgacgc gggccatatc
     2281 gtttgactgc aacgccgcat caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc
     2341 acccagcgcc cgcgcccaca actcttccag tttggcctta aaggtgcggt tgatgagcat
     2401 atcgcgcgga taaattttca atgcggataa ccgcgtccga ctctcttcaa tctgctgcgc
     2461 gctcaatgtt accgggctat gattaatgac tctggactta acgctgccat cttccagaag
     2521 cacgtcaact tcaagcaagc cgttgatatc ataactaaag cgaatatcaa tcgactgata
     2581 agccccggtt ttcttcaacg gcacatcgaa ggattccacc agaatgttat ttttaacttt
     2641 gtggttttct ccctgataga cgttaaccgt aatagaatcc tgttccgggt gcatggtcga
     2701 gtaagtttct acacgcgaca ccggtactgt ggtgtttcgt tcaataatcg gcgagaaaat
     2761 gccggaaacg ccctggcggt taacttcaac gcccaacgag taagggcaaa tatcagtgag
     2821 gatcacttct tcaatatctt cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag
     2881 cgcgacaatg gtgctcggat cgtaactttg atacggtaat ttgccaaaca gacgcacggc
     2941 gattcgctgc accagcggca tttgtgacgc gccgccaacc agcaccagac tgtcgatttg
     3001 actcggcttc agacgcgcat cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag
     3061 cagcggcaac cacaaatctt ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcatc
     3121 ctggtactgc cagcgaatgt gcattggcga ttgattgcta cattttgccg cttccacaca
     3181 ggcgtacaag gctgccagtt cactctcgtt aagcgtggtc ctggtgacat ccgcgcgctt
     3241 taaaacctca tcgaccaaca tatgggtaaa atcttcgcca ccgaggaagt tatcgccagc
     3301 ggaggcgtga acttcaatca ccggcgtggc gtactcaagc accgtgacgt caaacgtgcc
     3361 gccaccgaga tcaaaaacca gcgagcgggt attttgttgg gtatgcaggc catacgccat
     3421 cgcagctgct gtgggttcat taattaagcg taccgcattt aacccggcta actccgctgc
     3481 taaacgggta tgcttgcgtt gttcatcgct gaaataagcc ggaacggaga tcaccacatc
     3541 tttaatcgga cgttgcagaa attcttcggc atcttctttt aatgagcgta ataccaaaga
     3601 ggacagttct ggcgcgttaa atgtgtcgct gcctaaccgc cagttggtat tactgcccat
     3661 tgcacgttta aataacgctg ccgttttatc cggatgcgaa gtacgccgtg atacagccgg
     3721 ttttccgact aaaatatgat tattttcatc catgctaatt atggatggtg ttaaatattc
     3781 accgaactta tttggaatta attgcgcggc accgtctttc cagacggcaa ttaaactatt
     3841 ggtagtaccg agatcgatac caatggcgag ttctgcatta tccattgcat ttatatcttg
     3901 aaagaaaaag agataaacag attaagacca cccaggtaac gcgtggtgat cttaatcaat
     3961 gacgtgtgtt aagcgtaaaa tttcagacga tcagccagta aatcaacaaa gccctgacga
     4021 tcaacatcga ccattacagt ggcattcggt ttattgccag tcagataata ataatcaaca
     4081 accgtcatac cctgggtata tttcccctgt gtttccacgc caacccagcg ctcaacagtg
     4141 gtaaacaact ccggtttcaa cagccaggcg atggtgcatg ggtcatgcag cggtgcgccg
     4201 acaaagcccc atttttcgtc tttatgatat tcgaggaaga aatccagcag ttcggcaaca
     4261 atggttgaaa cagggttgcc aatcgcgcgg aaacgctcgg tgtcttcaac gtggatttgt
     4321 gctttgtgag taacatccag accggccatc accactggga tccctgactg gaagacaatt
     4381 tctgccgctt ctgggtcaac gtaaatatta aattcagccg caggcgtcca gttacccagc
     4441 cccattgcgc cgcccataat cacgatacgg gcaattttgc tatgcagttc cggatggcta
     4501 ttgagcagca aggcaacgtt agtttgcggt ccggtagaca caatcgtgac aggttccgca
     4561 ctttcgcgca gcgttttcgc catcagctct accgccgtac agttttgcgg cgcgaacgcc
     4621 ggttccggta atgccgggcc gtcgagaccg ctttcgccgt gcacattgtc cgcgataatc
     4681 aactcacgca ttaacggttt taccgcgccg cctgctaccg gaatatcggt gcgattgagc
     4741 aaggtcagca tacgcagaac attgcgtaag gttttttctg gtgtctggtt tccggcggaa
     4801 gacgtaattg ctttgacatc aagctctggt gaggcgaggg cgagaactat tgcgatagcg
     4861 tcgtcatgac ctgggtcgca atctaacaga attggcagtg ccattgttgc tccttgttgt
     4921 gtgcttcttt gcgacaaggg taacgccagg atgtaacaga tacgaggggc gaaacgataa
     4981 agcgtgagat ggcgcgcaat tgggtatgcg cgccagagtg attaatgcag gattttcgcg
     5041 aggaagtctt ttgcgcggtc cgatttcgga tcatcgaaga acgcatcttt cggcgagtct
     5101 tcgacaattt taccctcatc cataaagatc acccgattcg ccactttacg ggcaaaaccc
     5161 atttcgtggg tcaccaccat catggtcatt ccttcgtttg ccagttccac catcacgtcc
     5221 agcacttcgt tgatcatctc cggatccagc gccgatgtcg gttcgtcgaa cagcatggca
     5281 ataggatcca tacacaatgc gcgagcaatt gccacacgct gctgctggcc gccggaaagc
     5341 tgcgccggaa atttattggc atgtgcggaa agtcccacgc gctccagcaa tttcaatgct
     5401 ttttcacgag ccggcgcttt atcgcgttta agcactttca cctgcgccag ggtcaggttt
     5461 tcgataatcg acagatgagg gaacagctca aaatgctgga ataccatccc gacgcgggaa
     5521 cgcagctttg ccagatcggt tttcttgtcg ttaaccacga taccatcgac ggtgatttca
     5581 ccttgctgca ccggttcgag gccgttgacg gttttaatca gcgttgattt gccggaacca
     5641 gacgggccgc aaaccaccac cacttcgcct tttttcactt cggttgagca gtcggtcagc
     5701 acctgaaagt gaccatacca ttttgaaaca tttttcaggg taatcattat gctgtccttc
     5761 ttttcaagta gctgaccaac aacgatgcgc taaggctaat aacgaaataa acaaatccgg
     5821 caaacaggat catctcgacc tgcgtaccat cacgctcacc aatggttgag gcggtacgga
     5881 agaaatcggc cagagataac acatacacca gcgaggtatc ctggaacagt acgatgccct
     5941 gagtgagcag cagcggtacc atcgcgcgga acgcctgcgg cagaataatc agtttcatcg
     6001 actgccagtg agtcattccc aacgccagcg cggcgctcga ctgaccacga gaaatacttt
     6061 gaataccagc acggataatc tctgaatagt aggccgcttc aaacattgaa aacgccacca
     6121 tcgccgaaat taaacggata tcattttttg gtgataatcc cagcacgttt tgcagaaaac
     6181 ctggcacgat caggtaaaac cacagcaaaa ccataactaa aggaatcgag cggaatacgt
     6241 taacgtaggc tttggcaaac cacgctacgg gcgcaaagct ggacaagcgc atcaccgcca
     6301 gcatggtgcc ccacaaaata ccaatcacga ctgccgtgac ggtgattttc agcgtgatca
     6361 ccagcccgtc gagcagatat ggcagggaag ggacaatgga actccagtca aactcgtaca
     6421 ttatttgccc cccatgttgc caggcaggcg aactttacgt tcaaccagcg tcattaccag
     6481 catgataacc gcgttaatca acacatacgc cagcgtaata gcggtaaacg actcccaggc
     6541 gtgggctgag taatcaagca atttacccgc ctgcgccgcc atatccacca gaccaatagt
     6601 cgaggcgatg gcggagtttt tcaccaggtt catcatctcg gaagtcatcg gcgggacgat
     6661 aacgcgataa gcattaggca gcagtacgta tcgataagcc tgcggtagcg tcaggcccat
     6721 cgccagcgcg gcattttttt gcccacgcgg caacgactga atggcggcgc gtacctgttc
     6781 gcaaacacgg gcggcggtaa acagccccag gcagagcatg gatgaaagga aaaactgaat
     6841 attgggatcc agctcggcct taaaccacat gccgattttc tccggcaaca gctccgggat
     6901 caccagatac caggtaaaga attgcacaat caacggcacg ttgcggaaca gttcgacata
     6961 caacgtaccc agaccggaga gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga
     7021 accgacgagg aaagcgataa tccaggcgca gatcgacaaa gcgatcgtca cctgaaaacc
     7081 actccagatc caaccaagat aggtggtgtt gccgaacggg gcttgttgta aaaaaatacc
     7141 ccagttccag tctatagaca taaatctact ccagaaaaaa gagggtagca gcgttaactg
     7201 ctaccctcga agattgttac ccagcgtatt gcggttttca ggcccgatgg ggaacgacca
     7261 tcaggcgtat agtctgtccg tgctacgtaa caatcgagag ggctggaatt tccgcccctg
     7321 gttcttgtaa ttagttcagt gctttgtcat tcggttcttt gaacagtgct ttcatttcgt
     7381 ctgacagttc gaaattcatg ttcaggtttt tcggcggaat tggatttttg aaccatttat
     7441 caaaccattt ttccgcttca ccggaagtct gcacctgagc gatagtgtca tccatcagct
     7501 ttttgaactg cggatcatct ttacgcaaca tgcaaccgta ggcttcctga gactgcggct
     7561 tgccgacgat atcccagttg tctggtttct tcgctttcgc acgttcaccg gccagcaggg
     7621 catcatccat cataaaggca acggcacgac cgctttccag ggtacggaaa gagtcaccgt
     7681 gatctttggc gctaatgatg cgcatattca ttttttgctc ttcattcagt ttgttgagca
     7741 aaacttcaga agtagtgccg gaagtgacga ctacggcttt gcctttcagg tcggcaaaat
     7801 ctttgatatc gccgcccttt ttggtcagca ggcgcgtacc gaccacgaaa atagtatcag
     7861 agaaagccgc ctgtttttgg cgttcgacgt tgttggtggt agaaccacat tcaaaatcga
     7921 aagtgccgtt ttgcagcagt ggaatacggt tttgtgaggt aatcggaatc agttttacct
     7981 gcaagtccgg tttgttgagt ttctttttca ctgcttcaac aatggcgttg gagtaatcct
     8041 gcgagtaacc caccactttt tgctgattgt cgtaataaga gaaaggtact gaagattcac
     8101 ggtgaccgac gacaatcacg ccgtttttgg cgattttgtc cagcgtactg cccgctaccg
     8161 gggcggcgtc atctgcctgt gccagtcctg cggaaagcgc cagggcgagg attgctgtgg
     8221 caggtttacg taattgcata tccaactcct ttatcttctg cgttaagaac gcatggatac
     8281 ccgttgtgag tgtttgtgtt gttatcgtct gcaactttat tgtgcagtgt tgtgcctgtt
     8341 aggtaacatt tagtttggct aaatgtaaag atattgctgt tttattgttt gtttttgcga
     8401 gatgcgccgc accattccga agcaaaattc ttaaaccgca cctttttggt gctaccgctg
     8461 atttgtggtg gtgcaacctg gttgcgtcgc ggctgatttc atcgcttaca cagtaaataa
     8521 agcaacttac atgccaaaaa atacttaggg gcgaattaat tacgattgta attttaggct
     8581 gaaaaagtca tatttcggac agaattatct tttcggcatg ggacgtttgc cgggatgata
     8641 actgcacacg ggaaaaatgc tttttacctg tcttttcaag gaagaaacac cttatgtctg
     8701 catcttcggt taagccgtta aatgttcaat tacccgcaat aacccttatc ctttttgcac
     8761 tctgtattgg gatattttgt tatctcgcac aatggatgag ttatgaagag gtcgatcaat
     8821 ccgcacttat ccatctcggt gcaaacgttg cgcctcttac tttgtcgggt gaaccctggc
     8881 gcttattgag cagtatcttt ctgcacagta gtgtttctca tctgctgatg aatatgtttg
     8941 cattcctggt cgtgggaggc gtggcggaac agattctggg gaaatggcga ctcctgatta
     9001 cctggttatt ctccggcgtc tttggtgggc tcatcagcgc ctgttatgcg ttacgcgaaa
     9061 gtgagcagat agtcatcagc gttggggcat ccggggcgat tctgggaata gctggtgcag
     9121 cgatagcaac acagtttgct tcaggtgcgg gcacatacca taaaaaccag cggcgagtat
     9181 tttctctgtt gggtatggtg gcgctgacac tgttgtatgg tgcccggcaa acaggaatag
     9241 ataatgcttg tcacattggc ggcctgattg cgggtggcgc gttgggttgg ctgagcgcgc
     9301 gtttatctgg gcaaaaccga ctcgttacgg aaggcgggat tattgttgcg ggcagtcttc
     9361 ttctgaccgg ggctatctgg cttgcgcagc agcagatgga tgagtcagtt ttacaagtca
     9421 ggcaaagcct gcgtgaagcg ttttatccac agaagattga acaagagcga cggcaaaaaa
     9481 agcagcagtt agcggaggaa cgcaacgccc tcagggaaac attatccgct ccggtaagtc
     9541 gtgaacaggc cagtggtgat ttgctcgctg agattgccga tatccatgat atggcgatca
     9601 gtcgggatgg taatatgttg tatgccgcaa ttgaaaacac gaacagcatt gttgttttcg
     9661 acctcggaca aaagaaaatc ctgcatacct ttacagcgcc catagcgaaa gaaaagtcag
     9721 tcaaacattg tggtggctgt aaagatcagg gcgtcagatc gctggcatta agcccggatg
     9781 aaaagttgat ttatgcgact tcatttgaag cgaatgcgtt atcggtcatt aacgtggcga
     9841 caggggagat tattcagtcg attaccaccg gtgcacatcc tgacagtctt atcctctcgc
     9901 gtgatggcac aaaagcctgg gtgatgaatc gcaccagtaa tagtgtgtca gccattgatc
     9961 tggtggctta tcagcatgtg gcggatatcc cgctggagaa atacgacggg acggggacga
    10021 gcggtaaacc aggcgcctgg gttatggcac tttccccgga tgaaagaaca ttactggttc
    10081 cgggagaagg cagaggtaac atcgtgcgga tcaataccat cacgcatcag aaagaagact
    10141 ttcccgcagg tgatgcgcgt ggaacgatat cggcgatgcg ttttcgacct gaaaacggcg
    10201 aggttatttt tgccgatagt caggggattt cacgtataag cataggggat caacaaacca
    10261 gcattatgac gcaatggtgt agcaggagcg tttattccgt tgagggtatt agcccggacg
    10321 gtcaatattt agcgttggtg tcatatggac tgcaaggtta tgtcatcctg ctcaatatta
    10381 atgccgggca gattattggc gtttatcctg ccagctacgt taatcacctt cgtttttcag
    10441 cggatggtag aaaaatattt gttatggcga agaacgggtt gatccaactg gacaggacgc
    10501 gctcgcttga tccgcaggca attattcgtc atccccaata tggcaatgtg gcttgtatcc
    10561 ctgaaccgta agaaaaaggg gcatgataac catgccccaa agctcaatga gaaagtacgt
    10621 tttgctcatg caggatacat caggcacatc aacgtttacg tgctcgcaga ctcatcagca
    10681 cagcggcaaa accaaacagt gccgtcagca cccacagcgg ccagttgccg gttcgtgcgt
    10741 acggtgtgag tccggtggtc ggcgtcacgt tagtggtcaa cacctcgcgg gtgaactgcg
    10801 ggatcatcgc ctgaatctca ccctgcgggc caatcaccgc cgtaatgccg ttgttggtgc
    10861 tgcgcaacag tgggcgcgcc agctccagcg cacgcattcg cgccatctgg aagtgttgcc
    10921 atggaccaat agatttacca aaccacgcat cgttggagat agtcagcaga tagtcggtat
    10981 ccgggcggaa gttatcgcgc acttgctcgc caagaatgat ctcgtagcaa atggccgcag
    11041 taagctcaat accatttacc gacagcggcg gctggatata tggcccacgg ctgaacgacg
    11101 acatcggcag atcaaagaac ggtgctaacg gacgcagaat cgactccagc gggacaaact
    11161 cgccaaacgg caccagatgg tttttgttat agcgatcggc tgattcgtag ctgtacggtg
    11221 cacctttacc cagtgtgatg atggtgttgt aggtatcgta gcggttctgc ttattgagac
    11281 gcgcgtcgac aatcccggtt accagcgagc tacctttatc gcgcaactca ccgtccagtg
    11341 ctttgaggaa cggttgctga ttaatttcca gatcggttat cgccgactcc ggccagataa
    11401 tcaacgatga tttgcccatc agcggagccg ttgcgttgta gtaaatcttc agcgtattaa
    11461 gaagctgatc tccatcccat ttcagtgatt gcggaatatc gccctgaacc atcgaaacct
    11521 gaatggtttt ctccggttgt ggggtaaacc attggatgta acgcagcggg aagggtaggg
    11581 caaacagcac gacggccacc accagcggac gccagttgcg tttgaccaac gccagtgcca
    11641 gcaggccact aaccatcatc agcaggaagt taatggcttc cacgcccatt atcggtgcca
    11701 gcccttttaa cgggccatca atctggctat agccgaactg taaccacggg aagcctgtca
    11761 gtacccaacc gcgcagaaac tcggtcactt gccagagaga aggggcggca atcgccacgc
    11821 gcagccaggt ggttttcggc cacagacgcg acagcacgcc agcaaacagt ccggtataca
    11881 gcgacaaata cgccgccagc agcaccacca ggaagatgtt aaccgggcca ggcatcccgc
    11941 caaaggtcgc gatgctgaca tagacccagt taataccgct gccaaagagg ccaaatcccc
    12001 agcaaaagcc aatagcggca gactggagtg gacggcggtt aaaggtcaac gcctgaagcc
    12061 ccatcagcga aataatcgcc gcaggccaga catcgtaagg agagaaggcc agcgttccgc
    12121 aggcaccgaa taataacgcc agcagcaggc gaatgcgctg gcgttcaatt aatgaggcaa
    12181 aagccatgta gttatctatc cagtttcggt tttattcatc cagcttcggc tggggtgagt
    12241 catccgggat tttgacatga acctgaataa tacgccgact gtcggccatc gccactttga
    12301 actggtaacc gtcgatgtcg atagtttcgc cacgcgccgg aagatgccca aatgcctgca
    12361 tcaccagacc accgatagtg tcaacctctt catcgctaaa gtgggtgccg aacgcttcgt
    12421 tgaagtcttc aatggaagcc agtgcgcgca cggtccaggt atgacgactc agctgacgga
    12481 agtcgatatc atcttcttcg tcatactcgt cttcaatctc accaacaatt agttccagga
    12541 tgtcttcaat ggtcaccaga ccggaaaccc caccgaattc gtcaataacg atcgccatgt
    12601 ggtaacgctg agagcgaaac tctttcagca tccggtctac gcgcttactt tcaggaacga
    12661 caaccgcctg acgtaacact ttgtccatgc tgaaggcttc agcatcgctg cgcataaacg
    12721 gcagcaagtc tttcgccatc agaatccctt caatgtgatc tttgtcttcg ctaatcaccg
    12781 ggaaacgtga gtgggcggac tcgatgatga catcaaggca ttcgtccagc gtctggttgc
    12841 gtttcagggt aatcatctgg gagcggggga tcatgatgtc gcgaacgcgt tggtctgcga
    12901 tgtccatcac cccttcgagc atatcgcgcg tatcttcgtc gataaggtcg ttctgcccgg
    12961 aatcacggat cagcgccagc agttcgtcac ggtttttcgg ttcaccgtgg aaaagttggc
    13021 tgagtaacag ggagaaaaat cccttcttgt tgcttatcgt gtcactactg tgtgaattgt
    13081 cgtcgctcat ggcgtcgtat gggttctcat gttagttaat caaaacgccg tcgttaatca
    13141 ccaacggcgg ggacgtctgc cagtcaaatg cctggcaaat tattctttct cggcaatgta
    13201 cggatcctca tagcccagag caagcataat ctctgtttcg agggcttcca tttcttctgc
    13261 ttcgtcatct tcgatgtgat cgtaacctaa caaatgcaga ctgccgtgca ccaccatatg
    13321 cgcccagtgc gcctccagtg gtttgccttg ctcctgagct tccttctcaa ccacctgacg
    13381 gcagataacc agatcgccca gtagcgacat ttccatgcca ggcggcactt caaacgggaa
    13441 ggagagcacg ttggtcggct tatccttacc gcgataggtc agattcagac tgtggctttc
    13501 ggcggtatcg accacgcgaa tagtcacttc cgattcttcc tgaaactgcg ggatcaccgc
    13561 attcagccat gtttgaaact ggctctcttc cggtaacccg gaattatctt cacatgccag
    13621 ttgtaaatcg aggatcacct gactcatttt tgttcctgtt cttcgcgctt gcgttctgcc
    13681 gccagcgccg cttttcgttt ttgttcggct tcttcccagg cttcataggc gttaacgata
    13741 cgcgccacca cagggtgacg aaccacgtct tcgctgtgga agaagttaaa gctgatctct
    13801 tcgacatcgg ccagcacttc gatggcgtga cgtaagcctg atttagtatt acgcggcagg
    13861 tcgatctgtg tgacgtcgcc ggtgataacc gcttttgagt taaaaccgat acgggtcagg
    13921 aacatcttca tctgttcgat ggtggtgttc tggctctcat cgagaatgat aaacgcgtcg
    13981 ttcagcgtac gaccacgcat ataggccagc ggcgcgactt caataacgtt gcgctcaatc
    14041 agtttctcaa ctttctcaaa gcccagcatt tcaaacagcg cgtcgtacag tgggcgcaga
    14101 tacgggtcta ctttctggct taaatcgcca ggcaggaagc ccagtttctc accggcttcg
    14161 accgccggac gagtcagcag aatacggcga atttcctgac gctccagggc atcaactgct
    14221 gcagcgactg ccaggtaggt tttacccgta cccgccgggc caacgccgaa ggtaatgtca
    14281 tggtcgagaa tattggcgat gtactgcgcc tggtttggcg tgcgcggctt aattacgccg
    14341 cgtttggttt tgatattgac cgctttgccg tactccggca cgctctccgc gctttgctcc
    14401 agtacccttg cttctttaat cgcaaggtgg atctgttccg gttcgatatc ctgaatctga
    14461 ccgcgcatcg gggcagtatc gacatacagg ctacgcagaa tgtctgccgc agcggtgacg
    14521 caaatcggac ggccggtcag tttaaagtgg ttatcgcggc gattgatctc gatgccgaga
    14581 cggcgttcga gctgcttgat gttgtcatca aacgggccgc acaggctcaa cagacgcgcg
    14641 ttgtctgctg gctccagggt gatttcgcga gtgtctatgt tcaaaccgtt cctcttatct
    14701 gtatgccgcc ggaagctgaa cattcaccgg cctataagga aattattcac gccacaggaa
    14761 aaaggcgcaa gcattgcgat aaagatgggg ataaagagag aaaaaacaag gcccaccgga
    14821 acggcgggcc tgagaattac ggctgataat aacccacgcc aaggtcgttt tctttgcggg
    14881 tacgggcaat caccgattcc ggtgtttctg ccacgcgcag acccatttca tcttcagtac
    14941 gcaccacttt accgcgcaga gagttcgggt agacgtcggt aatttctaca tcgacgaatt
    15001 taccgatcat atccggtgtg ccctcgaagt tgaccacgcg gttattttcg gtacgcccgg
    15061 aaagctccat gatgctctta cgcgaagtcc cttctaccag aatacgctgg gtggtgccga
    15121 gcatccggcg gctccatgcc atcgcttgct gattgatacg ttcttgcaga atatacagac
    15181 gttgcttctt ctcttcttcc ggaacatcat caaccatatc ggcggctggt gtacccggac
    15241 gtgcagagaa gataaagctg tagctcatgt cgaaattgac gtcggcaatc agcttcatcg
    15301 ttttctcgaa gtcttcggtg gtttcgccag ggaagccaac aatgaagtca gaactgatct
    15361 gaatatccgg acgcgccgca cgcagtttac ggatgatcgc tttgtactcc agtgccgtat
    15421 gagtacggcc catcaggttc agaatgcgat cggaaccgct ctgtaccggc agatgcagga
    15481 agctcaccag ctccggcgtg tcgcgataca cttcgatgat atcgtcggtg aattcgatcg
    15541 gatggctggt ggtaaagcga atacgatcga tcccgtcgat cgcagcaacc agacgcaaca
    15601 gatcggcaaa cgatccggtg gtgccgtcgt agttctcacc acgccaggca ttcacattct
    15661 gaccgagcag gttgacttca cgcacgccct gagccgcaag ctgggcgatc tcaaacagaa
    15721 tatcgtcgga cggacggctt acctcttcac cacgggtgta aggcaccacg cagtaggtgc
    15781 aatatttatt gcagccttcc atgatggaga caaacgcggt cggcccttcg gcgcgcggtt
    15841 ccggcagacg gtcaaacttc tcgatttccg ggaagctgat atctacaacc gggctgcggt
    15901 cgccgcgcac ggagttgatc atctccggca gacggtgcag cgtttgcggc ccaaaaataa
    15961 tatcgacata gtgggcgcgc tggcgaatgt gctcgccttc ttgcgatgcc acgcagccac
    16021 cgacgccgat aatcaggtct ggattcttct cttttaacag tttccagcga cccaactgat
    16081 ggaagacttt ttcctgagcc ttctcgcgga ttgagcaggt gttcagcagc agcacatccg
    16141 cttcttccgc cacgtcggtc agttgatagc cgtgggtggc atccagcaga tcggccatct
    16201 tcgatgaatc gtactcgttc atctgacagc cccaggtttt aatatggagt tttttggtca
    16261 tcgacttgct cttgcgaaat agtagccagg aatgcagggc gcatagtgta atgctttgct
    16321 gccgttgtga ccagtatgag cgttatcagc ccttgggggt aaaaatcctg taaacttaaa
    16381 gcatattgct aacaggatga ttgaccatga caaatcaacc aacggaaatt gccattgtcg
    16441 gcggaggaat ggtcggcggc gcactggcgc tggggctggc acagcacgga ttttcggtaa
    16501 cggtgatcga gcacgcagaa cctgcaccgt ttgtcgctga tagccaaccg gacgtgcgga
    16561 tctcggcgat cagcgccgca tcggtatcat tgcttaaagg gttaggggtc tgggatgcag
    16621 tacaggctat gcgttgccat ccttaccgca gactggaaac gtgggagtgg gaaacggcgc
    16681 atgtgatgtt tgacgccgct gaacttaagc tgccgctgct tggctatatg gtggaaaaca
    16741 ccgtcctgca acaggcattg tggcaggcgc tggaagcgca tccgaaagta acgttacgtg
    16801 tgccaggctc gctgattgca ttacatcgag ataatgatct tcaggagctg gaactgaagg
    16861 gcggtgaagt gattcgcgcg aagctggtga ttggtgctga cggcgcaaat tcgcaggtgc
    16921 ggcagatggc gggaattggc gttcatgcct ggcagtatgc gcagtcgtgt atgttgatta
    16981 gcgtacagtg tgagaacgat cccggcgaca gcacctggca gcaatttacc ccggacggac
    17041 cgcgtgcgtt tctgccgttg tttgataact gggcatcgct ggtgtggtat gactctccgg
    17101 tgcgtattcg ccagttgcag aatatgaata tggcgcaact ccaggcggaa atcgcgaagc
    17161 atttcccgtc gcgtctgggt tacgttacac cgcttgccgc tggtgcgttt ccgctgacgc
    17221 gccgacatgc gttgcagtat gtgcagccgg ggcttgcgct ggtgggcgat gccgcgcata
    17281 ccatccatcc gctggcgggg cagggggtga atcttggtta tcgtgatgtc gatgccctga
    17341 ttgatgttct ggtgaatgcc cgcagctacg gtgaagcgtg ggccagttat cctgtcctca
    17401 agcgttacca gatgcggcgc atggcggata acttcattat gcaaagtggt atggatctgt
    17461 tttatgccgg attcagtaat aatctgccgc cgctgcgttt tgtgcgtaat cttggtttga
    17521 tggcggcgga gcgtgctggc gtgttgaaac gtcaggcgct gaaatatgcg ttagggttgt
    17581 agccttacaa cattgccgga tgcgtgccaa ccgtaggtcg gataagacgc ggcagcgtcg
    17641 catccgacat tgaaggataa gacgtgtcaa catcgcattc gacattgaat gaacgcagaa
    17701 aagcaaaaag ctcgccgaag cgagcttttt taat
//
LOCUS       DAPGWD010000084        17557 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPGWD010000084 DAPGWD010000000
VERSION     DAPGWD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.085"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(266..1279)
                     /locus_tag="RHX88_002767"
     CDS             complement(266..1279)
                     /locus_tag="RHX88_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV2231436.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIATGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     ASAKTLPGTTYTPIEVSCCSPTAKAIEKPVVTCCSTKAVVSINPIKLATKDALQQFKD
                     VLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLAS
                     VLMTKGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQ
                     LLF"
     gene            1386..1682
                     /locus_tag="RHX88_002768"
     CDS             1386..1682
                     /locus_tag="RHX88_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2231437.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1816..2241)
                     /gene="arsC"
                     /locus_tag="RHX88_002769"
     CDS             complement(1816..2241)
                     /gene="arsC"
                     /locus_tag="RHX88_002769"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDV2231438.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2254..3543)
                     /gene="arsB"
                     /locus_tag="RHX88_002770"
     CDS             complement(2254..3543)
                     /gene="arsB"
                     /locus_tag="RHX88_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDV2231439.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKAGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3597..3950)
                     /gene="arsR"
                     /locus_tag="RHX88_002771"
     CDS             complement(3597..3950)
                     /gene="arsR"
                     /locus_tag="RHX88_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDV2231440.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4690..4773
                     /locus_tag="RHX88_002772"
     CDS             4690..4773
                     /locus_tag="RHX88_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDV2231441.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4827..6179)
                     /gene="gorA"
                     /locus_tag="RHX88_002773"
     CDS             complement(4827..6179)
                     /gene="gorA"
                     /locus_tag="RHX88_002773"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDV2231442.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6251..7093)
                     /gene="rlmJ"
                     /locus_tag="RHX88_002774"
     CDS             complement(6251..7093)
                     /gene="rlmJ"
                     /locus_tag="RHX88_002774"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDV2231443.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7296..9338
                     /gene="prlC"
                     /locus_tag="RHX88_002775"
     CDS             7296..9338
                     /gene="prlC"
                     /locus_tag="RHX88_002775"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDV2231444.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVNELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9346..10098
                     /gene="rsmJ"
                     /locus_tag="RHX88_002776"
     CDS             9346..10098
                     /gene="rsmJ"
                     /locus_tag="RHX88_002776"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDV2231445.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10147..11616)
                     /gene="dtpB"
                     /locus_tag="RHX88_002777"
     CDS             complement(10147..11616)
                     /gene="dtpB"
                     /locus_tag="RHX88_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDV2231446.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11934..12368)
                     /gene="uspA"
                     /locus_tag="RHX88_002778"
     CDS             complement(11934..12368)
                     /gene="uspA"
                     /locus_tag="RHX88_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDV2231447.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12759..13094
                     /gene="uspB"
                     /locus_tag="RHX88_002779"
     CDS             12759..13094
                     /gene="uspB"
                     /locus_tag="RHX88_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDV2231448.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13165..14664)
                     /gene="pitA"
                     /locus_tag="RHX88_002780"
     CDS             complement(13165..14664)
                     /gene="pitA"
                     /locus_tag="RHX88_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDV2231449.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLNTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14896..16098
                     /locus_tag="RHX88_002781"
     CDS             14896..16098
                     /locus_tag="RHX88_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV2231450.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16414..17466)
                     /locus_tag="RHX88_002782"
     CDS             complement(16414..17466)
                     /locus_tag="RHX88_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDV2231451.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4572 a         4423 c         4505 g         4057 t
ORIGIN      
        1 aaaaatgtgt tgtattagcg actgaaatct gccttttaaa aaggaggagg gtactgagac
       61 aaagagtcat tgcttctccg ataaaatgtc agagaagcat gattacgtgg ttattgtctg
      121 ttgatgactt gttgaccgag ccgtcatcat tctcaatgca ttagttaaat catttgtgat
      181 ttttaaggat tatcctgtct atgattttct acttagaagg atatggcact aactatgcat
      241 cttatagttt actgctggca tctatttaaa atagcaattg agtcaaatag cccatcaaga
      301 tagccatacc taatataacc gtcaggaatg cagctatcat cggcattctg aacattgatt
      361 taagtaatat cacttccgtc aggcttgcgc cggcactgcc gatgattaat gccattaatg
      421 ctcccagacc cattcctttt gtcatcagaa cagatgccag agggataact gcctcagccc
      481 ggatatacag cggaataccg acaacggcgc ttaatgggat ggcgaaggga ttatctgctc
      541 ctgcatgagc ggcaatccac gctgagggaa taaagccata aataaaagat cctattaata
      601 ctcctaacaa aagatatggc agtacatctt taaactgttg caacgcatct ttggtggcta
      661 gttttatggg attaatactt actacagctt tggtactgca acaggtaact acgggtttct
      721 caatggcttt agccgttgga ctacagcaac tcacttctat tggcgtatat gtcgtccccg
      781 gcaaagtttt tgccgacgct ttggctggag cacaacaatt tgctgatgag cttttactgg
      841 caatgatatg acgttcaaac cccagagaat ccaggataat actggcaaga acggagacgc
      901 cggtggcgat aatcgcgtac aacaaggtga ctttccaacc aaaggtcacc cacattagtc
      961 cgacgataat gggattaagt aatggtgaaa caaataaaaa agtgagagtc ggaccaaacc
     1021 cggctttcgc tgacaacaat ccacgtagca tgggtattgt cgagcaacta cagaacgggg
     1081 taacggctcc caacagagca gccaggagat agcctctccc ttttctggcc cccatcatct
     1141 gctggatttt atggtctggc accttttgtc ttatcaggct gacaccggca ctaatcacta
     1201 taaacagcaa cgaaagctcg acggcgagaa acacaaacat ctctgcggca tcttgcagca
     1261 tcgcaagcca actactcata cgtaacctca ttggataaat ctatatttcc agaataatcg
     1321 aaatgaaatt cagatcaaga tatttctggt attatcgaaa tatcaaccag acagaggggt
     1381 gtcatatgca gttggaagaa gtcgctaaag cgctgaaaga gctaggtcac ccgacccgtt
     1441 tgttcatatt taaacatctt gtcaaagccg gggaacaagg gttgccggtc ggtgagttgc
     1501 aaaagcagct tgggatacct ggctccacac tcagccacca tatatcagca ctggtatcgg
     1561 taggactggt caaacaaaac cgtgagagtc ggacgctgat gtgtgtttcg caatacgcaa
     1621 tgctggaagc gataattgaa ttcttacgcg aggagtgctg cgtaaatagc aaaattgcct
     1681 gatgcgctac gctctacata tttcatgcca tatattgaat ttgcacggtt ttgtaggtcg
     1741 gataagacgc gttagcgtcg catccgacat aaacaaagcg cactttacca actatgggca
     1801 gtggatatcg ccgctttatt tcaaccgctt acccgcttca tcaaccactt tctcgccatc
     1861 ttccttggag aatgcgcctt tttgcgcatc tggcagaatt tccagcacca cttctgaagg
     1921 gcggcacagg cgagttccca gcggcgtcac cacaatcggg cgattaatca gaatcgggtg
     1981 ctgaagcata aagtcgatta accgatcgtc agtaaattta tcttctgcaa ggcccagttc
     2041 ctcatacggc tcgacgtttt tacgcaacag cgcgcgtacg gaaatcccca tatcggcaat
     2101 gagtttgacc agctcatcgc gcgttggcgg tgtttccaga taatagataa tggtcggttc
     2161 tgtgccgctg ttgcggatca tctccagcgt attacgcgac gtgccgcagg ccgggttgtg
     2221 ataaatggta atgttgctca tatcagtatc tcattacaaa gtgaaagaga gacgtagcgc
     2281 cagcgcagcc agcgtcacaa acagcacagg cagggtcatg ataatccctg tacggaagta
     2341 atatccccag ctgatagtca tattcttctg cgaaagtacg tgcagccaga gtagcgtagc
     2401 caggctacca attggggtaa ttttcggtcc caaatcgcag ccaatcacat tggcataaac
     2461 catcgcttct ttgataacgc cagatgccgt gctgccatca atggacaacg cgccaaccag
     2521 taccgtcggc atattgttca taatagaaga gaggaaggcg gtgaggaatc cggtgccgag
     2581 cgtcgcggcc cacaggccgt tatccgccag cacgttgagt acgccagaaa gatattccgt
     2641 taatccggca ttgcgcaggc cataaaccac cagatacatg ccgagcgaga agatgacaat
     2701 ctgccagggg gcaccgcgca ggactttacc cgtattaatc gcatgaccgc gtttagcgac
     2761 gacaaataat atcagcgcgc ccacagctgc aatggcgctc accggaatgc cgagcggttc
     2821 caggacgaaa aatcccacca gcagaagcag taaaacaacc cagccagctt tgaacgtagc
     2881 aggatctttg atcgcttctg cgggagattt cagcagcgcc atatcgtagt tctgcggaat
     2941 atctttgcga aaatagagat gtaacatcac cagcgtggca acaatcgcgg cgatatccac
     3001 cggcaccatc accgaggcgt attcgcgaaa gccgaggcca aagaaatcag cggaaacgat
     3061 attcaccagg ttggagacaa taagcggcag gctggcggta tcggcaatga atccggccgc
     3121 catcacgaac gccagcgtag tgcctttact gaaccctaaa gccagcagca tggcgatgac
     3181 aatcggtgtc aaaataagcg ccgcgccatc attggcaaac agggcggcaa cggcagcacc
     3241 gagcaggaca atccaggtaa acagcaagcg accacgacca ttaccccagc gtgagacgtg
     3301 cagcgccgcc cattcaaaaa agccggactc atccagcagc aggctgatga taatgacggc
     3361 gataaacgca gccgtcgcgt tccagacgat attccacacc accggaatat cacccggatg
     3421 gaccacgccc gtaactaacg ccagtactgc gccgagcgtt gcactccagc cgatgcctaa
     3481 acctttcggc tgccagataa ccaatacgat ggtcaggaca aagatagcgc ctgccagtaa
     3541 cataatgcct cccggataaa acacatctga aaattcatat gtgtttagct aaatttttaa
     3601 ctgcaaatgt tcttactgtc cccggaacag ttttgtcgag ccaggttgcg gacaatcgcc
     3661 tgaacctttt cctgttcaca tcgccaggcc tcatcaataa ttttcgccgc ccatgctgga
     3721 atatgcggtg ataagcggta atgaacccac ttaccttgct tgcggtccag caatagcccg
     3781 ctttcacgca gcaatgccag gtggcgggag atcttgggct gcgactggtc gagagcagtg
     3841 cagagatcgc agacgcataa ctctcccagt tcgctgagca gtaaaacgat gcccagacgg
     3901 gtttcatcag caagaatttt gaacaattgg atgggtaaca gaaatgacat attgcgctcc
     3961 tgattgttgc aggtagtgtc tctcttcgaa gcggataagt caaaaacata tatgacttaa
     4021 cgaatgtgta agtgcagagg aaggtaatag gtgtgaattt tgagttggct attcatttga
     4081 aaagaggtct gaatcaggac gtaaaaaaac cgactttgcg tcggtttttt actttccagc
     4141 cctgagttgg tggctctggc tggagtgaga gagttcttat ctaacagctc aatactatta
     4201 gccagtggct aacattgcaa gaattagcga tttcttcagc tggcggaggg ggcacttcgg
     4261 tatatatcgc aatgacgatt tatatacttt cactgggtca tcgtcatatt aagccttgtc
     4321 cgaatccgga tgtaaaaaaa ccgactttgc gtcggttttt tactttccag ccctgagttg
     4381 gtggcactgg ctggagtgag aatgccatta tctaacgcat caatgctaat accatactga
     4441 aactattgca aggacatgct ggttttataa cctgaatgta ctgtatgatt atccagttag
     4501 ctctgaggca ttttcactct ggtaatgcgc ataaacgctt tcaaagtcct ggtcagaagt
     4561 acgggtggtg ccgttaactg atgctctggc cggagtgaga atgccattat ctaacaatag
     4621 ggcatgcgcc gtgacaggca gtggatgagt aagcggatgc attctcactc catcgcatgg
     4681 agaaaacggg tgattgataa agcaatcatc gttcttgggg cgttaattgc gctgctggaa
     4741 ctgatccgct ttctgcttca gcttctgaac tgatagcgga aacgtaatta agggctaaga
     4801 gcacactact cttagccctt taacatttaa cgcattgtca cgaactcttc tgccgccgtt
     4861 gggtgaatgg cgacggtatt gtcgaagtct tttttggttg cacccatctt cagcgccacc
     4921 gcgaagccct gcaacatttc gtccatacca aagccaatgc cgtgaatacc gacaatcttc
     4981 tcttccgatc caacgcacac cagcttcatg cggcacggct ggcggtgagt ggtgacggcg
     5041 gtatacatcg cggtgaaaga ggatttatac actttcacct gatcgtcgcc atactgctcg
     5101 cgcgcctgcg gttccgttaa accaacagta ccaatcggcg gatggctgaa gaccacggtc
     5161 ggaatgttgc tgtaatccag atgctcatcc ggcttgttat taaacaggcg ttcagagaga
     5221 cggcgacccg ctgcaactgc caccggtgtc agctccactg cacccgtgtt atcgcccacc
     5281 gcgtaaatac cttcaatatt ggtgttttga tatttatcga cgacgatata gcctttttcg
     5341 ttagttttaa cgccagcggc ttccaggttg atgttgtcat tggcaggctc gcgaccaatc
     5401 gcccaaatca ggcaatccac cgtttcactg cgaccatctt ccagctccag cgtcaggcta
     5461 ccatcggcat ttttcactac cgctttcggg atggcgttgg tgtgcagctg cgggccttcg
     5521 gcgttcatca cttcgaccag cgtttcggaa atcatcgggt cgaagctgcg cagcggcgca
     5581 tgtttacgca caaacagatg cgttttcgcg ccgaggccgt taatcacgcc cgccagctca
     5641 acggcgatgt aacccgcgcc aacaaccgcc acgcgctctg gcaaagcagg aagggcgaag
     5701 aagccatcag aatcaatacc gtattccacg cccggaatat ccgggtggct cggacgaccg
     5761 cctgtggcga tcagaatatg atcggccgtg atggtttcgc cgtttacctc cagcgttttg
     5821 gcatcaacga agcgggcaaa gcctttgatt acatcaacgt tatttttacc gagcacgttt
     5881 tcataggaag tatgaatacg gtcgatatag gcggtacggc tggcgatcaa cgtttcccag
     5941 ttgaatttat tgatagtggt atcaaaacca taatccgggc cgtacatatg gatcgcttca
     6001 cggatttgcg ccgcgtgcca catcactttt ttcggcacac agccaacatt tacgcaggtg
     6061 ccgcccagct ctttggcttc aatcagcgca catttctggc cgtacatagc cgcgcggttg
     6121 atggaggcga taccgccgct gccgccgccg atggcgatgt aatcatagtg tttagtcatg
     6181 acaaagtgtc cttatcgttg attaccgcga ttgtagcgcg agacgtaata ggtgccagca
     6241 atggctgcaa ttactccggc acgatccagc ttacggtggc gtgcccggtg cctgccggaa
     6301 ccagtttgct gtgcagccac ggcagcacgt tattcatctg ctgttccagt ttccacggcg
     6361 ggttaatcac aatcatgccg gaagcggtca tgccacggcg atcgctgtct ggcagtaccg
     6421 ccagttcaat ttgcagaatt ttgcgaatac cggtcgcttc cagatcgtgg atcatgcgct
     6481 taatttgctg acgcagcacc accggatacc acagtgcgta agtaccagtg gcgaaacgtt
     6541 tgtaaccttc tgctatcccg ctgaccaccg cctgatagtc ggttttcatt tcatacggcg
     6601 ggtcgataag gattaaacca cggcgggaaa ccggcggcag tttggcctta agctgctgga
     6661 aaccatcggc tttttcgacg cgcgcacggc tgtctttctg aaattcagaa cgcagcaacg
     6721 ggtaatcgct cgggtgcagt tcggtcagtt gcaggctgtc ttgttcgcgc agtagctggc
     6781 gagcaatcaa tggcgaaccg gggtagtaac gcaactgacc gctacggttg aagtgtttta
     6841 ccacattgat gtacgcctcc agttctgcgg gcaaatcgtc ctgctgccag atacgggcga
     6901 tgccttcgag atattcgccg gtacgctcgg catgttcgct gcctaactga taacgcccgg
     6961 cccctgcgtg ggtgtcgaga tagagaaacg gtttatcttt ctctttcagc gactcgatga
     7021 tcaggctctg aacggtatgt ttaaggacgt cggcgtggtt gccagcgtga aagctgtggc
     7081 gataactgag catgggtaaa ggtgttccgg taagtaaaaa tcggcccgta ttcggggcgc
     7141 acaaaagcgt atcaggacag tataccgaaa agaggccgcc gccgcgaaag cgtaacgttt
     7201 ctcattgaaa ttcactacac ttaaccccat gctacacaca ttatgtaaag cgcctgttga
     7261 gcgcttcctt aacctcttta accaggactg cgcgaatgac gaatccgtta ctgactccct
     7321 ttgaattgcc tccgttttct aaaattctcc cggaacatgt cgttccagcc gtgactaagg
     7381 cgctgaacga ctgccgcgaa aatgtggagc gcgtagtagc gcaaggagca ccgtacacct
     7441 gggaaaatct ctgccagccg ttggcggaag tggacgatgt gctggggcgt atcttctccc
     7501 cggtcagcca cctgaactcg gtgaaaaata gcccggaact gcgtgaagcg tacgaacaaa
     7561 ccctgccgct gctgtcggaa tacagcacct gggtagggca acatgaaggg ctgtataaag
     7621 cgtatcgcga cctgcgcgat ggcgatcatt acgccacgct gaacacggct cagaaaaaag
     7681 cggttgataa cgcactgcgc gacttcgaac tctctggcat cggtctgcca atagagaaac
     7741 aacaacgcta cggtgagatt gccactcgtc tttccgagct gggcaaccag tacagcaaca
     7801 acgtcctcga tgcgacgatg ggctggacca aactcgttac cgacgaagcg gagctggcgg
     7861 ggatgcctga aagcgcgctg gctgcggcaa aagcccaggc cgaagcgaaa gagctggaag
     7921 gctacctgct gacgctggat atcccaagct acctgccggt aatgacctac tgcgacaacc
     7981 aggccttgcg tgaagagatg tatcgcgctt acagcacccg cgcctccgat caaggcccga
     8041 acgccggtaa atgggataac agcaaggtga tggaagagat cctcgctctg cgtcacgaac
     8101 tggcgcaact gctgggcttt gaaaactacg cctttaaatc ccttgccact aaaatggcag
     8161 aaaacccgca gcaggtgctg gatttcttaa ccgatctggc aaaacgcgcg cgtccgcaag
     8221 gcgaaaaaga gctggcacaa ctgcgcgcct ttgccaaagc cgaatttggc gtcaatgagt
     8281 tgcagccgtg ggatatcgcg tactacagcg aaaaacaaaa acagcacctc tacagcatca
     8341 gcgacgagca gctgcgtccg tacttcccag aaaacaaagc ggttaacggc ctgtttgaag
     8401 tggtgaaacg tatttacggc atcaccgcta aagagcgtaa agatgttgat gtctggcatc
     8461 cggatgtacg tttcttcgaa ctgtatgacg aaaataacga actgcgcggc agcttctacc
     8521 tcgatctgta tgcccgtgaa aacaagcgcg gcggggcgtg gatggatgac tgcgtaggcc
     8581 agatgcgtaa agctgatggt tcgctgcaaa aaccggtcgc gtatttgact tgtaacttca
     8641 accgcccggt aaatggtaaa ccggcgctgt tcactcacga cgaagtgatc accctgttcc
     8701 acgagttcgg tcacggcctg caccatatgc tgacccgcat cgaaaccgct ggtgtttccg
     8761 gtatcagcgg tgtgccgtgg gatgcggtcg aactgccgag tcagtttatg gaaaactggt
     8821 gctgggagcc ggaggcgctg gcgtttatct ccggtcacta tgaaaccggc gaaccgctgc
     8881 cgaaagagtt gctggataaa atgctggcgg cgaagaacta ccaggcggcg ctgtttattc
     8941 tgcgtcagct ggagttcggc ctgtttgatt tccgccttca tgccgagttc cgcccggatc
     9001 agggggcaaa aatcctcgaa actctggcag aaatcaagaa actggttgcc gtggtgccat
     9061 ctccgtcctg gggccgtttc ccgcacgctt tcagccatat tttcgccggt ggttatgcgg
     9121 caggttacta cagctacctg tgggctgacg tactggcggc agatgctttc tcgcgctttg
     9181 aggaagaggg cattttcaac cgtgaaaccg ggcagtcgtt cctcgacaac attctgagcc
     9241 gtggcggttc agaagagccg atggatctgt tcaaacgctt ccgtggtcgt gaaccgcagc
     9301 tggatgcgat gctggagcat tacggcatta agggctgatc attcagtgaa aatctgctta
     9361 attgatgaaa caggcgccgg agacggcgcc ttatctgttc tggcggcccg ctgggggctg
     9421 gagcacgatg aagacaacct gatggcgctg gtgttaacgc cggaacatct ggaattgcgc
     9481 aagcgtgatg agccaaaact tggcggcatc tttgttgatt ttgttggcgg agcgatggcg
     9541 caccgacgca aattcggcgg tggtcgcggt gaggcggtgg cgaaagcggt gggcattaaa
     9601 ggcgattatt tgccggatgt ggtggatgcc accgcagggc tggggcgcga tgcctttgtg
     9661 ctggcttcag tcggctgccg cgtgcggatg ctggagcgta atccagtcgt tgccgcattg
     9721 ctcgacgacg gcctggcgcg tggttatgcg gatgcggaaa tcggcggctg gttgcaggag
     9781 cggttgcagt taattcacgc ctccagcctg acggcgctga ctgatattac cccgcgcccg
     9841 caggtggttt atctcgaccc gatgttcccg cataagcaga aaagcgcgct agtgaaaaaa
     9901 gagatgcgtg tctttcagtc gctggtggga ccggatcttg atgccgatgg attactggag
     9961 cctgcacgct tgctggcgac caaacgcgtg gtggtaaagc gcccggacta cgcgccaccg
    10021 ctggcgaatg tcgccacgcc aaacgcggta gtcactaaag ggcataggtt tgatatttat
    10081 gcaggtacgc cagtgtaaaa gtaaaccagg gtagcgataa cgctaccctg ctttctgcaa
    10141 gaataattaa tggctttccg gcgtcgcaat catgcgtttc agccacggca ccatcaacag
    10201 catcactact gcaacgccca gtgtgaccag accaatctta ccaaacacgt tggtatagac
    10261 gggcaacgtc tcaagcggat cggtaatgtt gtccggcacc gcggtaaatg ttgccacata
    10321 gccgcccagc aagaacgcgg cagcctgcgt caggaaccac atcccgagaa taaagcccat
    10381 caaatgctgc ggcaccaggg cagcaatcat cgccaggcca agggcgctaa taaacagttc
    10441 acctaagctc tggaataagt acaccagcac gataaaccat ggcgatgtca gcccctgggc
    10501 atccgcaaac cacattcccg cagctgcagc cgtcaaaaag cccagagagc acataaacat
    10561 gccgagagta aatttcatcg gcatcgagag gtctttgcct ttgttaccca gatgcgtgta
    10621 aatgcctgcc agtattgggc tggcgagtac cacccagaac gggttaagcg cctggaagct
    10681 gaccgggttg atggaaaaac cgagaatttc atgatgcacg ttgttgatgg caaagaagtt
    10741 cagcgatgtt ggcatctggg cgtagagaat gtaaaacacc accgcttcga gcatcaggac
    10801 aaaggcgaca aacattttat tgcgcccggt tttatccagc ttgaatgcct gacgaaagaa
    10861 gatgatggtg acgacgatgg agagaacaat cagcaccaga ttggcgactt ctacgttgtg
    10921 catcagccat gcacatacga agatcatcac cacgctgcca agtaatacgt acaacagttt
    10981 gctgaagctc atcggcttga agtcgggttc agaaccaatg tctttcacca ttccacgaca
    11041 ggcgatgtaa accagtaatg cgataattaa ccccgccccg cacaggttgt aggtgactga
    11101 ataaccgaat ctatcagcga tcacaggggc cagcgataac gctatcaacg agccgatatt
    11161 gatcgacata tagaacaggg tgaatgcgcc atcaagccgc ggatctttcg gcggatagca
    11221 cttcgaaagc aagctggctg ggttagcttt aaacaggccg ttaccgacag cgatagtccc
    11281 cagggcgatg aaaatcaggt caggcttaag tagcgacatg ccggtcatga agtagccaat
    11341 cgccagcaca agtgctccga gaacaatggt gcgtttggtc cccagcaggt ggtcgccgac
    11401 atagccgcca atggaaatga ggccatagac cagcgcagca aaagcaccaa aagtgacaaa
    11461 agcctgctct tgcgagaatc caagctgttt aacgaagaaa accgccagta cgccctgcac
    11521 gccgtagtag ccgaatcgct cccataactc gacaaaaaag atcatgaaaa atgggcgagg
    11581 ttgctgcagc atccccatgg gtgttgttgt attcatattt attaaccttc caataccatc
    11641 ctgaaaagta tgaacgcgta agggataacg cgcgaaaaag tttacatctg tcagagattc
    11701 ttaatattga tgcggtgaat agattaatgc actattacaa cagaaaataa ccagctgatc
    11761 acactaatga tggaaaatat gatggtgtta aatgttgtat tggcaggaga ttttgattgc
    11821 gtacgcagat cgtaggccag ataaggggtt tacgctgcat caggtaacaa ggcgcgccaa
    11881 aaagcaaaac gcccgcatat gcgggcgttc aggaacacgt cgtggaggga tgattattct
    11941 tcttcgtcgc gcagcggaac aatcagcata tcaacgtgaa cggtgttgat cagctgacgt
    12001 gcggaagaca tcagtttgct ccagaagtcc tggtggtgac cacaaaccac cagatccata
    12061 tcgtatttct tgattgcatc gaccagaacc tggcccaggt cgccgctgcc gctcagggtt
    12121 tcagtgattg ggtagcctgc attagtggaa agctcggtca gcgcatgatg tgtctcttca
    12181 gagatgcgtt tctgcatatc acccagattc acatcaataa gcccggtgta taggtcagag
    12241 tagtttacat ctacgtggat cagagaaact ttcgcattgt aggggcgagc catagagact
    12301 gctttctcta ccagaacttt gctttccggg gagagatcga ctgcgatgag aatgtgttta
    12361 taagccatag tgttactcct tccataaagt tgtcgatgac tggccagcta gcgtttcttg
    12421 tgccgttacg tcacccgcgt cctgcgaact acgtgcgcgg ggctaaccca gccgtaaaga
    12481 ttcagtgtta agaccatcct taccttatag cgacccggat gatccgtcaa tccgccttgc
    12541 ttacccgtta gataaacaaa ttttcgacat gaatatattg atagtggtta accttctgga
    12601 aaaaaaacaa cctgatctcc tacactatct atagagccgc tcggatgttg gtcaccacaa
    12661 aagcggtttc tccaggacct ggtgttaact gattggcagc gtatcgggag cggtagcccc
    12721 ggggtggaac tccgcgggca ggtcgccggg gaggaggtat gataagcacc gtcgcattat
    12781 tttgggcttt atgtgtcgtt tgcattgtta acatggcgcg ctatttctca tcactacgcg
    12841 cgttgttagt ggtactgcgt aactgcgatc cattgctcta tcaatatgtt gatggagggg
    12901 gcttttttac ctcacatggc caacccaaca aacaggtgcg tctcgtttgg tatatctatg
    12961 cccaacgtta tcgcgatcat cacgatgatg aatttattcg ccgctgtgag cgggtgcgtc
    13021 ggcagtttat tctgaccagc gcattgtgtg gtctggtggt ggtcagcctg attgcattga
    13081 tgatttggca ttaagcactt cagcaataat aacgcataaa aaaagcgggc cagtaaactg
    13141 acccgctcgt tttggtgtgt acgattacag gaactgcaag gagagccagt acagcccgcc
    13201 ggaaagcagt accgcagccg gaagggtaaa cacccaggcc atcagaatgc tggtcacggt
    13261 tttacgctgt aagccgccac catctaccac catcgtcccc gcgacagaag aggagagtac
    13321 gtgagtagtg gaaaccggca tcccggtata actcgccagg ccgatagaca ctgccgccgt
    13381 catctgggca gacatcccct gagcgtaggt catgcctttc ttaccgattt tctcaccgat
    13441 agtcgttgcc acacggcgcc agccaatcat cgtaccgata cctaacgcca gcgcgaccgc
    13501 catgatgatc cacaccggtg catactcgat ggtgctaagc atgtcagact tcagtttctt
    13561 caacaggcgc tgatcgtcag cactcacgcc aggcatcttc accactttgt cgatagtgtc
    13621 agaaacgcac agcataatgc ggcgcatctg gctacgttga tcaagcgaca gcttgtcgta
    13681 gctttccaca tcggtgttca acataccttt caggcggttg agcgcgttaa tggtattcga
    13741 cggatggcag tggaactccg caggttgcgt tgcgccagct tccggagccg gtactaactg
    13801 atcagcaccg gtagcctgtt tgagcagcgc aggatgctgc tcaaagtaag cttcgacgtt
    13861 gttgatggca tcacgggtac gggtgatttc gtagccagtg gcattcatgt tcaccacgaa
    13921 gcctgctggc gcgacgccaa tcaataccaa cataaccaga ccaatgcctt tctgaccatc
    13981 gttcgcgccg tgcgaaaacg ccacgccgat agcggaaagg atcagcgcga tacgcgtcca
    14041 gaacggcggc tttttcttgc cgtctttctt ttcacgctcc gctggggtca ggtggatacg
    14101 ggcgcgtttc ttggtgccgc tccagtagcg acgcagcaag aaaatcagac cgccagcaaa
    14161 caccaggccg acaatagggg aaacgatcag agaaccgaaa atacttaata ctttcgggat
    14221 attgagtgca tccaccactg acgtcccggt catcaacgca ttggttaaac caatcccgat
    14281 gatcgcgcca atcagcgtat gagagctgga tgcaggtaaa ccaaagtacc aggtacccag
    14341 gttccagata atcgccgcca gcaacataga gaacaccatg gcaaggccat gagacgatcc
    14401 catattaagc agcagatccg tcggcagcat atgcacaatg gcataggcaa cactcagacc
    14461 acccagcaaa acacccaaaa agttgaacac cgccgccata accacggcga gctgagaacg
    14521 catcgcgcgg gtatagataa cggttgccac ggcgttggct gtgtcatgga aaccattgat
    14581 ggcttcgtag aacagcacaa aagccagtgc aagcaataat aacagcccgg tatgcaaatc
    14641 caggccagca aacaaatgta gcataggacg ttacgccatt ttgaggacat gaacgcggcg
    14701 cattatcagt gactttcgcg gcgcgggcaa agtgaaatat agactttttt tgatttgcct
    14761 cctgtatgga tttcactcaa aaaataatta tcttatataa ttcaggcaaa tacttccttt
    14821 tagtaatatt gatgctggtg cgaccactga ggaatcttta caattcacgc ccgttttttc
    14881 taagaggagc gcaacgtgga aaggtttgat gccattatta taggcgctgg tgcggcgggt
    14941 atgttctgtt ctgcgctggc aggtcaggca ggacgccggg ttctgctgat cgataatggt
    15001 aaaaaaccag ggcgcaaaat ccttatgtct ggcggtgggc gctgcaactt taccaacctt
    15061 tatgtcgaac caggcgctta tctgagccag aatccgcatt tttgtaagtc tgcactcgcg
    15121 cgttttaccc agtgggattt cattgatctg gtcaataaac acggcatcgc ctggcacgag
    15181 aaaacgttag gacaactctt ctgcgatgac tccgcgcagc agattgtcga catgctggtg
    15241 gatgagtgcg agaagggcaa tgtaaccttc agattgcgta gcgaagtgct gagtgtggcg
    15301 aaggatgaaa caggcttcac gcttgaactg aacggcatga ctgtcggttg cgaaaagctg
    15361 gtcatcgcga ccggtgggct gtcaatgccg gggctgggcg cgtcgccgtt tggttataag
    15421 attgccgaac aatttggcct caacgtgctg ccgacccgtg cgggtctggt gccattcact
    15481 ctgcataaac cgttgctcga agagttacag gtgctggcgg gcgtggcggt gccttccgtg
    15541 attaccgctg aaaacggcat cgttttccgt gagaacttac tcttcaccca tcgcggcttg
    15601 tctggaccgg cggtgttgca gatttccagc tactggcaac cgggtgaatt tgtcagtatc
    15661 aatctgctac cggatgtgga tctcgaaacc ttcctgaatg agcagcgtaa cgcacatccg
    15721 aatcagagcc tgaaaaacac actggcggtt catctaccga agcggttggt tgaacgctta
    15781 cagcaactcg ggcaaatccc ggatgtttcg ctaaaacagc tcaacgtgcg tgaccaacag
    15841 gcactgatta gcacattgac cgactggcgc gtacaaccca acggcactga aggctatcgc
    15901 actgccgaag tgacgctcgg cggcgtggac accaacgaac tctcttcacg gacgatggaa
    15961 gcgcgcaaag tgcctgggct gtacttcatc ggcgaagtga tggacgttac cggctggctg
    16021 gggggctata acttccagtg ggcgtggtcg agtgcgtggg cttgtgcgca ggatttgatt
    16081 gcagcgaagt cgtcctgacc tgttacctaa agatgaaaat tattagagcc gcattaaaaa
    16141 tgagctaatt ttgatagtgg ctatcttgtg attattttct aatgagcccg tgaactgaaa
    16201 ccctccaggc ttaatataag gtggaaggaa aggtgattga aaactcaccc agtggccaga
    16261 tctttataaa atatgaacag gttgaaaaaa acagtaactt tgttgttttt ttatccttaa
    16321 ccactatgca ttaatgctgc gttatttcat gatgcctaag aaaaaccagc gttacgcaaa
    16381 tggtcaacgc tggttttatc cggtacgttg caattatttt ttagcagaac ctgcttctaa
    16441 tattgaaacg attgagaaca acgtaaagca taccgctcct aaaccgacca aaacccgcga
    16501 aggaataaaa tatcccatgt cggtctgcgc catttccgca agaaacgatg ccaggaaaag
    16561 gcaaaacagg caggtgaaga cgggaatcat cggtattctg ttggctaacg aacaggtacg
    16621 tctccatacc agtgccagta gccagacttt tgagaatatg ctgtaacaga tcattccgag
    16681 gcaaataaga ataatgccgg gagccagtcg ggcgcttgca tcggaactga ctaagacgta
    16741 tatcccctgc agtaccgtga ttgagccgag aaaaataacc caataacacc acagccagtg
    16801 ttctttagtt gaaaacgtat tacgtgtttg atggacaatt gtggcaacaa ggccaattag
    16861 acaggcacag attgcggtta acccaagcaa tacgtgaccc gcgacataat gcggagttat
    16921 gtcagcacta cgtaacagcg taatagacca aatgaaaccg agcagggtga gtaaaacggg
    16981 aacggcaatg aggcaattac cgattaatga agaataagcc tgtaccggcg ttccatcgct
    17041 cttgctcccc gctgcatttt tgggaatgag cagaaagtga ccggatgacg ctgccaccgt
    17101 cgatacacag gcggcaatca taccaacgcc gaaaataaca tggccggcga caaactcgtc
    17161 tgccatcaca tcattgcctg ctaacagtgc ccaaccccat atcatggtga taattgaccc
    17221 cgcatagcca ataatgggga acaatgtatt gtaaaacgta ttaacgccgc gcgtgagctg
    17281 cgaaatgata ataaatgcgg tagtgaataa tgccaggcat atggctgcca gagaaatcag
    17341 cacatgaccc gcaacgaaat actcactttg cccagaactt tccagcacaa agccacctaa
    17401 cgcgatacaa attaagccca taattaaagg gattaatttg aaaagccacc cgatatatat
    17461 gttcacttta aactcctcag gtatccctgc taatattgaa atgtgcatta atgataattt
    17521 cagcctgtta atataatgac gagatatcat taaagac
//
LOCUS       DAPGWD010000085        17539 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPGWD010000085 DAPGWD010000000
VERSION     DAPGWD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.086"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            75..1214
                     /gene="arnB"
                     /locus_tag="RHX88_002783"
     CDS             75..1214
                     /gene="arnB"
                     /locus_tag="RHX88_002783"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDV2231452.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALKIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1218..2186
                     /gene="arnC"
                     /locus_tag="RHX88_002784"
     CDS             1218..2186
                     /gene="arnC"
                     /locus_tag="RHX88_002784"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDV2231453.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2186..4168
                     /gene="arnA"
                     /locus_tag="RHX88_002785"
     CDS             2186..4168
                     /gene="arnA"
                     /locus_tag="RHX88_002785"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDV2231454.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4165..5055
                     /locus_tag="RHX88_002786"
     CDS             4165..5055
                     /locus_tag="RHX88_002786"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDV2231455.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTLVDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGKPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5055..6707
                     /gene="arnT"
                     /locus_tag="RHX88_002787"
     CDS             5055..6707
                     /gene="arnT"
                     /locus_tag="RHX88_002787"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDV2231456.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIAAFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWTLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6704..7039
                     /gene="arnE"
                     /locus_tag="RHX88_002788"
     CDS             6704..7039
                     /gene="arnE"
                     /locus_tag="RHX88_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDV2231457.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7039..7425
                     /gene="arnF"
                     /locus_tag="RHX88_002789"
     CDS             7039..7425
                     /gene="arnF"
                     /locus_tag="RHX88_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDV2231458.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7419..7685)
                     /gene="pmrD"
                     /locus_tag="RHX88_002790"
     CDS             complement(7419..7685)
                     /gene="pmrD"
                     /locus_tag="RHX88_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDV2231459.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7794..9149)
                     /gene="menE"
                     /locus_tag="RHX88_002791"
     CDS             complement(7794..9149)
                     /gene="menE"
                     /locus_tag="RHX88_002791"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDV2231460.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENSFPALTSLHIQRVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPIELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPHELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9146..10108)
                     /gene="menC"
                     /locus_tag="RHX88_002792"
     CDS             complement(9146..10108)
                     /gene="menC"
                     /locus_tag="RHX88_002792"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDV2231461.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDALPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVTEEGVRAVVIKPTLTGSLEKVREQVLAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(10108..10965)
                     /gene="menB"
                     /locus_tag="RHX88_002793"
     CDS             complement(10108..10965)
                     /gene="menB"
                     /locus_tag="RHX88_002793"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDV2231462.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10980..11738)
                     /gene="menH"
                     /locus_tag="RHX88_002794"
     CDS             complement(10980..11738)
                     /gene="menH"
                     /locus_tag="RHX88_002794"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDV2231463.1"
                     /translation="MNLHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11735..13405)
                     /gene="menD"
                     /locus_tag="RHX88_002795"
     CDS             complement(11735..13405)
                     /gene="menD"
                     /locus_tag="RHX88_002795"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDV2231464.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13494..14789)
                     /gene="menF"
                     /locus_tag="RHX88_002796"
     CDS             complement(13494..14789)
                     /gene="menF"
                     /locus_tag="RHX88_002796"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDV2231465.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLSPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14868..15173)
                     /gene="elaB"
                     /locus_tag="RHX88_002797"
     CDS             complement(14868..15173)
                     /gene="elaB"
                     /locus_tag="RHX88_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDV2231466.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVSRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15228..15689)
                     /locus_tag="RHX88_002798"
     CDS             complement(15228..15689)
                     /locus_tag="RHX88_002798"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2231467.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15754..16671
                     /gene="rbn"
                     /locus_tag="RHX88_002799"
     CDS             15754..16671
                     /gene="rbn"
                     /locus_tag="RHX88_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDV2231468.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16859..>17539
                     /locus_tag="RHX88_002800"
     CDS             16859..>17539
                     /locus_tag="RHX88_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDV2231469.1"
                     /translation="MMVTVVRNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHAATN
                     SPLKPAGPDNSEDLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIDCYLQKIAANAKNTEVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPKAVQLF"
BASE COUNT         4123 a         4735 c         4429 g         4252 t
ORIGIN      
        1 aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg tattcaatgg
       61 cggaaggaaa agcaatgtca gaatttttgc ctttttcgcg accagcaatg ggcgtggagg
      121 aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt ccgaagaatc
      181 aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc gcggtcagtt
      241 cagccaccgc cggaatgcat atcacgctaa tggcgttgaa aattggcaag ggcgatgaag
      301 tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg ttgggtgcaa
      361 cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa gcgatcgagt
      421 cagccataac gccacgcact aaagccatca ttccggtgca ttatgccggt gcgccagcag
      481 atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc gaagatgctg
      541 cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt accgctattt
      601 tttcatttca tgccattaaa aatattacct gtgcggaagg tggcctgatt gtaactgata
      661 atgaaaacct tgcccgccag ctacggatgc tgaaatttca cggtctgggt gtcgatgcct
      721 atgacagaca aacctggggc cgtgcaccgc aggctgaagt cttaacaccg ggctataagt
      781 acaatctgac cgatattaac gccgcgattg ccctgacaca gttagcaaaa ttagagcacc
      841 tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca gctctcccct
      901 ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg tttattattc
      961 gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg ttaaaagaaa
     1021 gaggcattgg taccgggtta catttccgcg ccgctcacac acaaaaatat tatcgcgagc
     1081 gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc tgttctttgc
     1141 cgctgttccc ggatatgact accgccgatg ccgaccgtgt catcacagcc cttcagcaac
     1201 tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg gtggttattc
     1261 ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc acagcctgtg
     1321 aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc gataattccg
     1381 cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct attttgctta
     1441 accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt accggcgact
     1501 taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc cgcctggtgg
     1561 caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc caggacagct
     1621 ggtttcgtaa aacagcttcg aagatgatta accggcttat tcagcgcacc accggcaaag
     1681 cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc gatgcgatgt
     1741 tgcactgcca tgaacgtagc acctttatcc cgattctggc gaatatcttc gcccgccgtg
     1801 ccattgaaat tccagtacat cattccgagc gtgagtttgg tgaatccaaa tacagtttta
     1861 tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg ccgctacgta
     1921 tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg gtgctgctgg
     1981 tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt atgctatttg
     2041 ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattactc ggtgaatata
     2101 tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag caagttatcc
     2161 gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc acgatatggg
     2221 atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca tttttaccca
     2281 taccgataat cccggtgaaa aagcctttta tggttcggtg gctcgtctgg cagcggaaag
     2341 aggcattccg gtttatgcgc cggatgacgt taatcatccg ctgtgggtgg aacgcattgc
     2401 ccaactgtcg ccagatgtga ttttctcttt ttattatcgc catcttattt gcgacgaaat
     2461 tttgcagctc gctcccgcag gtgcatttaa tctgcatggt tcgctgttac caaaatatcg
     2521 tggtcgcgcg ccgctgaact gggtgctggt caacggtgaa acggaaactg gcgttacatt
     2581 gcaccgaatg gtgaaacgtg ccgatgccgg ggccattgtg gcgcaactgc gcattgccat
     2641 tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct
     2701 actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga
     2761 aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg aatggcataa
     2821 accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt
     2881 cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag
     2941 caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg
     3001 cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt
     3061 agcgcagacg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc
     3121 ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac
     3181 agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc
     3241 gataagccgt tttctgaatc atccgcattt tcactttgtt gaaggcgata tcagtattca
     3301 ttccgaatgg attgagtatc atgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat
     3361 agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga
     3421 gaatctgcgc attatccgct actgcgtgaa gtaccgtaag cgaatcatct tcccgtcaac
     3481 ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat
     3541 cgtcggcccg gtgaataaac cacgctggat ttattcggta tcaaaacaat tacttgatcg
     3601 ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc gcccgtttaa
     3661 ctggatggga ccgcgactgg ataaccttaa tgcggcacga atcggcagct cccgcgctat
     3721 tacgcaactc attctcaatc tggtagaagg ttcaccgatt aagctgattg atggcggaaa
     3781 acaaaaacgc tgctttactg atattcgcga tggtatcgag gcgttatacc gcattatcga
     3841 aaatgcggga aatcgctgcg acggtgaaat tatcaacatt ggcaatcctg agaacgaagc
     3901 gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca
     3961 tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agcagctact acggcaaagg
     4021 atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccaccgct gcctggactg
     4081 ggagccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac
     4141 cgttgatctt acggataaac catcatgacc aaagtcggct tacgcattga tgtcgatacc
     4201 tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt
     4261 caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg
     4321 gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg
     4381 gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat
     4441 atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga tcaccatgcc
     4501 tggcaagccc atagcggtaa ctgggatcgg caaacattag tcgacgatat tgcacgcggc
     4561 cttcgcaccc tggaagagat tatcggtcaa ccggtaacct gctccgcagc cgctggctgg
     4621 cgtgccgacc agcaggtaat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat
     4681 tgtcgtgggg cgatgccgtt tcgtccattg ctcgaatcag gaaaacccgg tactgcgcaa
     4741 attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat
     4801 tttaacggtt ggctactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt
     4861 catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct gaaacgcgca
     4921 gcccaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagggac gttgccgctc
     4981 ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt
     5041 gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg tttattgcct
     5101 gctattacct gttaccgatc agtacgcgtc tgctatggca acccgatgaa acgcgttatg
     5161 cggaaatcag tcgggaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc
     5221 tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggt caatggctat
     5281 ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg
     5341 cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca
     5401 cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc
     5461 cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac
     5521 agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg
     5581 tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag
     5641 cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg
     5701 tactgacggt tctcccctgg ggactggcga tagcgcagcg ggagcctgac ttctggcact
     5761 attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa catagagctc
     5821 cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc
     5881 ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga
     5941 gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggcaaattg cccacctata
     6001 ttctttcctg ctttgctcct ctggcaatgc tgttggcgca ttacgctttg ctggcagcaa
     6061 aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg
     6121 gcattattgc cgcatttgtg gtctccccct ggggaccaat gaacacgccg gtgtggcaaa
     6181 ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg
     6241 gctggtacac cttaacaaac gtcgaaaaga cctggtcttt tgccgcgctt tgcccgctgg
     6301 ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga aaacatccgc
     6361 aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata
     6421 gcgtcggtgt tgccgcaggt ctggcatgga ccctgcaacg cgatgacatc atcatgtatc
     6481 gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca
     6541 gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attacactcg
     6601 tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca
     6661 tcgatcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt
     6721 agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt
     6781 tgtggcgata agcaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg
     6841 tcttggcctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc
     6901 ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca
     6961 cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggtg gcattgtgat
     7021 cctcgggagt acggtgtaat gggcctgatg tggggattat tcagcgtcat tattgcctca
     7081 gttgcgcaat taagcctggg ttttgcggcg agtcatctgc cgccgatgac gcacttatgg
     7141 gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg
     7201 ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact tgcgctaagc
     7261 aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt
     7321 cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc
     7381 gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccact
     7441 ttacactgcc gttcccactc atcggggcta taacagcttg cagagaggac tttcaccgta
     7501 tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc
     7561 actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg
     7621 cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt gaccagccat
     7681 tccattgcat tatcctgttt gctaagagtt ttcacatcaa tttacagatt aacagataaa
     7741 ctctttatca acggcaatgg aatggctgaa atgagaagtg aacggagggt taattattgc
     7801 tgacgttgca cccactcttt tagcgcctga cgtgaaattt taataccgcc gtttttcagt
     7861 tcatgcggca acgttaacca acgcaccggt tgctgaaaac gggccagctt atctttcacc
     7921 cattcactaa gatcaacgct ctcgtggtca tactccatca ccgccaccgg tcgatgacca
     7981 aactccttgt cggcaacggg gacgataaac acctgcaaaa ccgcaggatg tgcagcaatt
     8041 acgcgctcga cttcttccgg ctgaataccc tctccgccac tgaagaatag attgtctaaa
     8101 cgtccgacaa tggtcagctt gccattatgc atctcaccgc gatcgcgcgt agcgtaccag
     8161 ccttcgtcat taaccagtga aaccagttgc ccgttacgcc agtaaccttc tgccatactg
     8221 gcagcccgca gccacacttc attattaacg attttcactt cccgacccgg cagcggcgaa
     8281 ccaacgtctg ccaggccgtc ggcctctttc gcacacaccg tggaggcaaa ctcggtcaga
     8341 ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct gttccgtcaa ctcgatcggg
     8401 atagccgcgc cgccaagtaa caccgctttc agggaaacgg aactgcggtt aaccagcaaa
     8461 cgccagagtt gtgttggcac cagtgaagcg tgagtacagc ctgccagcat ttgctccaat
     8521 ggctgtttat cacgcaccgt catccgcgca ccagcgtata accagcgcca cataattccc
     8581 tgaccggaga cgtgaaacag cggtaaagag agcaaccagt catcgtgatc gccaaacgga
     8641 atcaacgata acaccccttc ggcactggca agatgggcct gataagtatg gacagcggct
     8701 ttcggcaacc cggtagaacc cgaggtcaac gtcattgagc acagacgcgt cggctgccac
     8761 gcagcggcat gtgcgccttc aacccgctga atgtgcagcg acgttaatgc cggaaacgag
     8821 ttttccccat ccggcaccag cgcaaattgc agcgtcagat tgggcagcaa ttcttcaagc
     8881 aacggttgcg gcagctgagg gttcacgggc aacacccgcg ccccgcattg cagtaacgcc
     8941 agccaggcga gcagcgtttg cggcgtattc cacgcccgca acatcacgcc gctgccctca
     9001 accacaccct gcaccgcaaa tccggaggct aattcatcga cgcgagcaca aagctcgcgc
     9061 cagttgagtt gctcgtcatt aagacgtaag gcgatggctt ctccccgcac ttgccgccaa
     9121 tgacgccacg gccagtcaga gaagatcata acagccgctc cagtgcatca acgtccacta
     9181 acggcagcgg gctacccggc cagcgacgaa cctgctgcgc ctgcatcaga tccagtgtgt
     9241 ccagccccgg aatggtgtct ggcgttaacc aggcggcaat ccgcgccagt tgcgttaagc
     9301 ctaagctcga ctcaatagaa gaactgatca ccgccgtcag ccccaacgcg tgcgccgcca
     9361 gtacctgctc gcgtactttt tccagactgc ccgtaagcgt gggtttgata actaccgcgc
     9421 gcacgccctc ttcagtcaca aaggcaaaat ccggctcgcg cagactttca tcccaggcaa
     9481 tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc gcgggttttg cacggctctt
     9541 cgagaaacgc gatgcggtcg cgataatccg ggttaacgta tttggcaaac tgctgacctt
     9601 tcagcggtgt ccaggcgcga tttgcgtcaa gacgcaaatg cagatccgga attgcctcca
     9661 gcaacagatt aaccaccatg ccgtcgcgca cggcttcgta caatcccact ttgaccttcg
     9721 ccactttctc gcccggcata tcggcaagtt tgaggatcag atcgtccgga tcgccattac
     9781 acagcggtgc cgcacggtag ttggctgctt gcggcaacgc atctgtcagt tctgccaatg
     9841 cacagcttac gccaaaggcc acggaaggca tctgcggtag ctcgcaatcg cctgccagcc
     9901 agttatttac ccaggcaagc agcacgcttt gcgcctcttc ccaggtttcc tggctgaagc
     9961 ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc ctcacgcagg caaacgtaca
    10021 gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccac ccccgcgtcc atggggatct
    10081 gccagcggta tacctgcgcg ctacgcatta cggattccgt ttgaatttgc tgaagtcagg
    10141 ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct tcttccgtca tgtagaacag
    10201 catggtggcg ttgcccgcca gctcctgcag ccccgcctgc ccgtcacagt cggcgttcag
    10261 cgcagctttc aggcagcgca gcgccatcgg gctgttttgc agcatttcgc ggcaccaacg
    10321 gacggtttct ttttccagat ccgccagcgg taccacggtg ttgacaaggc ccatatccag
    10381 cgcctgtttt gcgtcgtact gacggcacag gaaccagatt tctcgcgctt ttttctgccc
    10441 gacgatacga gccatgtagg aagcgcccca gccgccgtcg aaggaaccga ctttcgggcc
    10501 agtctgaccg aagatggcat tatctgctgc gatagtcagg tcgcacatca tgtgcagaac
    10561 gtgaccaccg ccgatggagt agccagccac catcgcgaca accggtttcg gacaggtacg
    10621 gatctgacgc tggaagtcca gcacgttcaa gtgatgtacg ccggaatcat ctttatagcc
    10681 gccgtaatca ccacgcactt tctggtcacc accggagcag aacgctttat cgcctgcgcc
    10741 agtcagaatg atcacgccga tattgtcgtc ataacgcgca tctgccagtg cctggatcat
    10801 ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc ggacgattaa tggtgatttt
    10861 tgcgataccg tcggtggatt tttcataacg aatgtcctcg aaaccttcgg agcagtcgtg
    10921 ccattcaacc ggtgcgtaaa gcattgcttc atcaggataa atcatattgt gtcctttagt
    10981 cagaaacgca agatctgcgc cagacttgcg attacgccag cgggattttc ccgatgcgcg
    11041 ttatgtccgg cgcgaggaat gacatggcag tcggcagcca gttccgccgc cagggcgcgg
    11101 aatttgctgt cacgttcacc acataaataa taaaacgcaa atgtgcgggc gctaaggtta
    11161 gcacgtaaat caggctggac ggcgagagaa gtcgcctcca gcatggcggc aagggttgcg
    11221 ccattattgt tgctgcgcag cgccaccagc tcccggcgtt gatcgtcatt gagcgaggca
    11281 aaaacaggct gttgatacca gtcggcaaat accgccgtta acggttctgt gcaaaaacgc
    11341 tgcgcccatt ggcgatcgga acgctgacgt tccgcacgtt gttcagcatt ttgcagcccc
    11401 ggatgcccgc cttcgacaat aaccccacaa agccccgcca gcccctggca agccgccatc
    11461 atcgccaccc gtccgccaag cgagtacccc accagccaga agtcaaggat gttgtaacta
    11521 accaaggttt tacgcagtaa gtcggtgaca tcatcaaatc catcgacgct aatcgccgcc
    11581 gaaccaccat gacctgggag atcaacatac aaccgtgagt agtcggcaaa cgcctcgccc
    11641 acttcttgcc attcgtggca atcgccggaa aaaccgtgca aaaacaccag ccagggtaaa
    11701 cctggttttc cgtgttttgc ctgcgcgtgc aggttcataa atggcttacc tgcgccagaa
    11761 gttgctggag cgtttgcgca ccatcggtgt cgttaaccac catttcaatc accgtggtgg
    11821 ttggcgtgcg ccaggcgtcg gcaaatgccg tttcaagttc ctgccagttt tgcggacgat
    11881 gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg gacgttttgc ggcatcagat
    11941 agaaacgctc acgctcgctt tgcggcgttg gcaacagcga gaaaatttgc ccgccgttgt
    12001 tgttcaccac aattaatacc agcggcgcag aaacctgacg caataacgcc agcgcgttga
    12061 gatcgtaaag tgcggagaga tcgcccacaa tcgccagcgt cggtttgccg cttgcccgct
    12121 gaacgcctgc ggcggtcgaa agcagtccgt cgataccgct ggcccctcgg ttgctgtaca
    12181 ccgggtaacc tgccggaagt tgcgaaagcg catcaatcag acgtaccacc aggctgttac
    12241 caacaaacaa ttgcccctgt tcaggcagat agtcgctgat gcgatgcgcc agttgcgctt
    12301 cgccaaacgc atcacggcgg gcaataaccg cctgcattgc ctgttccgcc aggcgcggga
    12361 tttcaacgca ccagggctgg cgtttttctg ccggatgcag ctccagccag tcggcaatat
    12421 tggcaattaa gcgacgtccg cgatggtgtg ccggatcaag tcgcccttca atgtcatcaa
    12481 caatccagta ctcttcgggt tcacagcttg cctgccattg caggagccgt ttccccgtca
    12541 ggctgcttcc cagttgcacc acaatttgcg cctgctgcag ctcgctggtc gctttggcat
    12601 tgcctaacca aagatcggca cacggcagcg gctgcccggt ttgtgacagc acatcgccaa
    12661 tcagcggcca gccaagagtt tgcgcccaca gggcaacttt tttgccctct tccgcactca
    12721 tgcgcccggc aaccaccacg ccgcgctttt gtcgccagaa gaaccagtcg cgctgttttt
    12781 cactttccag acgaggcgct tcacgcagcc acggtttgtc gtcctgccac cagtcaccca
    12841 gacgctgttg ccagctaagc ccggtatcgt ccatttcgcc atacagcggt tcagcaaacg
    12901 ggcagttgat atggactccc cccgcatgaa gcgtaccgag agcgtggtcg atggtagaaa
    12961 ccagccaacg tgcggggata tcccgggtcg ggcgcggcaa tgaaatactg tgcgtggggt
    13021 gagaggcgaa cattcccggc tggcgaattg cctgattcgc gccgcagtca attagctccg
    13081 gcgggcgatc ggcggttaac aggatcagtt tttctccggt taacccagct tcaatcagtg
    13141 ccggatagag atttgctacc gccgtgccgg aggtcacaat caccgccacc ggctgcttgc
    13201 tgactttcgc cagccccagc gccagatgtc ccagtccacg ctcatcgaaa tgggtgtggt
    13261 gaatgaatgc ggaattctcc gccgccgcta acgttaacgg tgtagaacgc gagccagggg
    13321 cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc cagaatgacc gccgcccagc
    13381 gtcggttaaa tgcgcttact gacatgagtt tgtccggtat caatattgcg gctaagtata
    13441 aggagctaca aaaatagatt attgatatga atcggtaatg atgcgactca ttactattcc
    13501 atctgtaata aagtacgcag ccctgccgct ttgttgtcga tttcctgcca ctcttgctcg
    13561 gggtcggaac cacggacaat gcccgcgcca gcatataatc gcacgacatt gccgctaatt
    13621 tttgctgagc gcagggaaac gcagaattcg ctttgttgta atgagagata gcccgcagaa
    13681 ccggcgtacc attctcgggt gaacggttcg tgacgggcga taaactgtcg cgccagatcg
    13741 cgcggtaagc cagcaactgc tgccgttggc tgcaactgat gtaaacagat cacatcatcc
    13801 gctttgttga gtgaagtcca gatacagcgg cgaagatgct gcactttacg cagacgcagt
    13861 acctgcggcg gtaaaacatc cagcgtctgg gtatcggcct gtaatcgttg gcagatatct
    13921 tccaccacca gcatgttctc gcgctggttt ttatcatccg ccatcagcca ctctcctaac
    13981 tgctgcgcct gcttatcatc aggattattt gctactgttc ccgccagcgc ttcagtacgc
    14041 agcgctttgt cacgccgccg ccataaccgt tccggtgaag agccaagaaa agcattttcg
    14101 ccatcaaagg ccatgtaaaa atggtagcaa ttcagattca gtcgacgact ggcagccatc
    14161 atcgccgccg cgttgaccgg acttgcgaaa tgcaggtcag ttgcccgagc gagcaccact
    14221 ttgtcgagct caccttcggc gatggttttc gttgccagtt cgattaattg cgtccagccc
    14281 gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat gtaacccagg caagggcttg
    14341 atactcacca gtgtggcgat aaattctttt gcctgaatcg catcgtgctg aagggagctt
    14401 tcgctgaata gcgtcagccg cagcgtggct ttaccgccac agcgtcgcca ttccaggcgg
    14461 ggtgaaagta aattgccctg cgacgggtca aaagcattca gcccccaaat gcgtaagtcg
    14521 gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt ccaacgacgt aaaacgggta
    14581 atcgcgccca ggacggcagc ttcttcatca ccattacgtt gttgccagta gaattgcggg
    14641 tacgtttgct gactggccag ccagctcaag gcatcaaaag cgtctttgag agggaaagga
    14701 atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt gcgacaaatg gcgcagtaga
    14761 ttttccagcg ccgtagtaag tgattgcacg cggacctctc cctgttaaaa gcctcacatt
    14821 atacggggta ctacaaaaaa aatgcagtac cccggtgtag ggaggtttta acggcgtgcc
    14881 agcaacagtc ctagtaccag cccaacggcc gcgcccacac caattccttg ccagggtttt
    14941 tcgtggacgt agtcatcagc acgagaaact gcctgcttcg cccgatagta ataactgtct
    15001 gaagcctggc taacccgttt tttcacatca tccagcgctt tttctgcacg cgctttcagc
    15061 tcaacatatt tctgatcggc gggatcgcca gaggagcgga gcacctcttc cagtgtttca
    15121 ctaagcagcg tcaggtcgtc atcgatacgt gtatcaccaa actgattaga cattctcgtt
    15181 ctccatttgc gtaaaacctg tcactaacta tagacaaggg ttgttgatta cgcctgaatt
    15241 acctcgcgtg ccatcccaat gtgtgggata ccatcttctt catagacctc cgtcacaggg
    15301 ataaaaccga aactctggta gaagttttgc agatgcgcct gcgccccgag gtaaacaggc
    15361 ttatcaggcc agtgatgcgt acatgtttcg agtgttttac tcatcagctg ctgccccact
    15421 ttttcgccac gcaacgcttc actgacaatc acccgaccta taacgaccgg ctcaagatca
    15481 tcatcacttt tcagaatcct cgcatacgcc accagttcat catttttcca accgagaata
    15541 tggcgattat cccccgtcaa atcatcacca tcgatatcct gataagggca attctgctcg
    15601 acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa gctgggaaac cgataattca
    15661 gagtgatgca gatcttgcca ttcaatcata atgctctcct tttagatgta tgccttatac
    15721 tagccggaat attttttgaa acgtgatgaa ctcatggaat taattttttt aggtacttca
    15781 gccggtgtgc caacccgcac gcgcaatgtc acggcaatat tgcttaacct gcaacatccg
    15841 acccagagcg gactttggtt gtttgactgc ggtgaaggca cccagcatca gctactgcat
    15901 accgccttta accctggaaa actggacaag atttttatca gtcaccttca tggcgatcat
    15961 ctttttggtt tacccggctt gctgtgcagt cgttctatgt caggcattat ccaaccctta
    16021 acgatttatg gtccgcaagg tatccgtgaa tttgtggaaa ccgcgctgcg gattagcggc
    16081 tcatggaccg attatccgct ggaaattgtc gaaattggcg ctggcgaaat tctcgatgat
    16141 ggcctgcgca aagtaaccgc ttatccgctg gaacacccac tggaatgtta tggctatcgt
    16201 attgaagaac atgataaacc gggtgcatta aatgcccagg cattaaaagc tgctggcgtg
    16261 ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa caatcacgct ggaagatgga
    16321 aggcagatta acggcgcaga ttacttagct gctccagtgc caggtaaagc gctcgctatt
    16381 ttcggcgata ccggcccctg cgatgccgca cttgacctgg ctaaaggtgt cgatgtcatg
    16441 gtgcacgaag cgacgctgga tataaccatg gaagccaaag ccaatagtcg cggccatagc
    16501 tctacacgcc aggctgcgac actagcccgt gaggctggag tcggtaagct aatcattacc
    16561 cacgtcagct cgcgctatga tgacaaaggt tgtcagcacc tgttacgtga atgcaggtca
    16621 attttcccgg cgactgaact agcgaatgat ttcaccgtgt ttaacgttta actctgctta
    16681 ttattaaaaa cagggcgaaa cttgccctgt tatcgcaacc cgcgctacat atatccaccc
    16741 acacgggcat attgagcgat ttttgatatt tattcatctc ctcacgcact atattcagag
    16801 caataagttg cctctattta tcttgattca gcacaacctt tgcaactaaa aggagcccat
    16861 gatggttaca gttgtcagaa attattgtca attatctcaa acgcaactca gtcagacatt
    16921 tgcagaaaaa tttactgtga ccgaggaatt actgcagtct ttaaaaaaaa cagcgttatc
    16981 cggagatgaa gaaagcattg agttactgca taatattgcg ttaggctatg atgaatttgg
    17041 aaaaaaagct gaagatattc tttaccatat tgttagaaac ccaacaaatg agaccctatc
    17101 gattatccga cttatcaaaa atgcttgttt aaaattatat aatttggcac atgccgcaac
    17161 caactccccc cttaaaccag ctggtccgga taattcagag gatctcctgt tcaaaaagct
    17221 attctcccct tcgaaattaa tgacaattat cggtgatgaa attcctctta tatctgaaaa
    17281 acagtcgctt tcgaaggtgc ttttaaatga tgagaataat gaactgagtg atggtacaaa
    17341 cttctgggat aaaaatcgtc aattaaccac agatgaaata gattgctatc ttcagaagat
    17401 cgccgctaat gcaaaaaata ctgaagtcaa ttatcctact ggtctctacg tcccctactc
    17461 caccagaact cacctggaag acgctctcaa tgaaaatatt aagagcgatc catcatggcc
    17521 gaaagcagtc cagttattc
//
LOCUS       DAPGWD010000086        17534 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPGWD010000086 DAPGWD010000000
VERSION     DAPGWD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.087"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(308..1336)
                     /gene="epmB"
                     /locus_tag="RHX88_002801"
     CDS             complement(308..1336)
                     /gene="epmB"
                     /locus_tag="RHX88_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV2231470.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1378..1944
                     /gene="efp"
                     /locus_tag="RHX88_002802"
     CDS             1378..1944
                     /gene="efp"
                     /locus_tag="RHX88_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV2231471.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1996..2121
                     /gene="ecnA"
                     /locus_tag="RHX88_002803"
     CDS             1996..2121
                     /gene="ecnA"
                     /locus_tag="RHX88_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV2231472.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2232..2378
                     /gene="ecnB"
                     /locus_tag="RHX88_002804"
     CDS             2232..2378
                     /gene="ecnB"
                     /locus_tag="RHX88_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV2231473.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2560..2877
                     /gene="sugE"
                     /locus_tag="RHX88_002805"
     CDS             2560..2877
                     /gene="sugE"
                     /locus_tag="RHX88_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV2231474.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2874..3407)
                     /gene="blc"
                     /locus_tag="RHX88_002806"
     CDS             complement(2874..3407)
                     /gene="blc"
                     /locus_tag="RHX88_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV2231475.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3496..4629)
                     /locus_tag="RHX88_002807"
     CDS             complement(3496..4629)
                     /locus_tag="RHX88_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV2231476.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTTGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNHRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4692..5051)
                     /gene="frdD"
                     /locus_tag="RHX88_002808"
     CDS             complement(4692..5051)
                     /gene="frdD"
                     /locus_tag="RHX88_002808"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV2231477.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(5062..5457)
                     /gene="frdC"
                     /locus_tag="RHX88_002809"
     CDS             complement(5062..5457)
                     /gene="frdC"
                     /locus_tag="RHX88_002809"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV2231478.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5468..6202)
                     /gene="frdB"
                     /locus_tag="RHX88_002810"
     CDS             complement(5468..6202)
                     /gene="frdB"
                     /locus_tag="RHX88_002810"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV2231479.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6195..8003)
                     /gene="frdA"
                     /locus_tag="RHX88_002811"
     CDS             complement(6195..8003)
                     /gene="frdA"
                     /locus_tag="RHX88_002811"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV2231480.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8328..9305
                     /gene="epmA"
                     /locus_tag="RHX88_002812"
     CDS             8328..9305
                     /gene="epmA"
                     /locus_tag="RHX88_002812"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV2231481.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIDALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9524..11026
                     /gene="yjeM"
                     /locus_tag="RHX88_002813"
     CDS             9524..11026
                     /gene="yjeM"
                     /locus_tag="RHX88_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV2231482.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11177..14500)
                     /gene="mscM"
                     /locus_tag="RHX88_002814"
     CDS             complement(11177..14500)
                     /gene="mscM"
                     /locus_tag="RHX88_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV2231483.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14522..15490)
                     /gene="psd"
                     /locus_tag="RHX88_002815"
     CDS             complement(14522..15490)
                     /gene="psd"
                     /locus_tag="RHX88_002815"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV2231484.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRGEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15587..16639)
                     /gene="rsgA"
                     /locus_tag="RHX88_002816"
     CDS             complement(15587..16639)
                     /gene="rsgA"
                     /locus_tag="RHX88_002816"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV2231485.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16734..17279
                     /gene="orn"
                     /locus_tag="RHX88_002817"
     CDS             16734..17279
                     /gene="orn"
                     /locus_tag="RHX88_002817"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV2231486.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         4059 a         4517 c         4594 g         4364 t
ORIGIN      
        1 gttgttaaaa taacataaat agccgtactc actctatata aacagaaaag aaaggttaat
       61 tattggtgtt ggctatataa agagtaacaa cagcaatgta tatgaataat taaaggttat
      121 gcaacgggca aagattaaat ttcccagttt aatgacaggt tgctgtactt gcgtttgttg
      181 ctcttgccgg atgcggcgta aacgccttat ccggcctaca gttcattgca aatccattca
      241 atttcaatgc acaaggcctg ataagcgtag cgcatcaggc taatgtttaa cttccctgtt
      301 taatcagtta ctgctggcgt agctggagat ccagcggcgt tttgctgggt tcgccgccga
      361 tttctcgcgc cagtttcggc accagataac ccgacaccag tgtcagcaac tcacgcataa
      421 tctgccgtgc ttcgtcatca ctcaccataa aatgcgccgc gccctgtact ttatcgagca
      481 catgcaggta atacggcatt acgccggcat cgaacaacgc attactcagg tttgccagcg
      541 tttgtgcgtt atcgttcaca ccacgtaaca gaacgctctg gttaagcagg gtgacaccga
      601 cacggcgcaa tttagccatc gcctgacgga acgtttcatc tacctcattg gcatgattga
      661 tgtgattcac cagcaagatt tgcagcgtag aacgggcaaa gcgttcaacc agcgcctcgg
      721 tgatacgtgc cgggatcaca atcggcagac ggctgtgaat ccgcagacgt ttgatatgcg
      781 ggatggcttc cagttgtgtg agcaaccagt ccagctcgtg atctttcgcc atcagcggat
      841 cgccgccgga gaaaatcatc tcgtccagtt ccggatgcgc agcaacatac tcaagagcag
      901 tctgccagtt acgcttgttg ccctgatttt cggcataggg gaagtggcga cggaagcaat
      961 agcggcaatt taccgcgcag ccgcctttga ccagcaaaag cgcccggttg tggtatttat
     1021 gcaacaaacc aggcactacg ctgtgctgtt cttccagcgg gtcggtggag aatccggacg
     1081 cgacgacaaa ctcatcttgc gaggtaagta cctgacgcaa aagaggatcg tccggattgc
     1141 ctttctccat gcgatcgata aatgagcggg gcacacgcag ggcaaacagc tttttggcgc
     1201 tgcgtccggc caacagtttt tcgtccgcgt ctatattcaa aagacgcaga agttcatcag
     1261 gatcggtcac aacatcggca agttgcgtta accaatcttc tctggatggg gtatttaggg
     1321 ttacaatatg cgccattttg tggcttagct accaattaac aaatttcaga gggccttatg
     1381 gcaacgtact atagcaacga ttttcgtgct ggtcttaaaa tcatgttaga cggcgaacct
     1441 tacgcggttg aagcgagtga attcgtaaaa ccgggtaaag gccaggcatt tgctcgcgtt
     1501 aaactgcgtc gtctgctgac cggtactcgc gtagaaaaaa ccttcaaatc tactgattcc
     1561 gctgaaggcg ctgatgttgt cgatatgaac ctgacttacc tgtacaacga cggtgagttc
     1621 tggcacttca tgaacaacga aactttcgag cagctgtctg ctgatgcaaa agcaattggc
     1681 gacaacgcta aatggctgct ggatcaggca gagtgtatcg taactctgtg gaatggtcag
     1741 ccgatctccg ttactccgcc gaacttcgtt gaactggaaa tcgttgatac cgatccgggc
     1801 ctgaaaggtg ataccgcagg tactggcggc aaaccggcta ccctgtctac tggcgctgtg
     1861 gttaaggttc cgctgttcgt acaaatcggc gaagtcatca aagtggatac ccgctctggt
     1921 gaatacgtct ctcgcgtgaa gtaatgcggt tgtggtgcgg cctgcaggct gcaccatcac
     1981 ttattcaggt cagagatgat gaaacgcctt atcgttcttg ttttgcttgc cagcacgctg
     2041 ctcacgggct gtaacaccgc tcgcggtttc ggcgaagaca tcaaacatct cggcaactcc
     2101 atctctcgcg ctgccagcta atttttcttc tcttccgaaa aatcatcaga tttccgtcat
     2161 ttttggcgat gttgtctatt attaatttgc tataggcaaa cataaataac attacctaaa
     2221 aggaagacgt tatggtgaag aagacaattg cagcgatctt ttctgttctg gtgctttcaa
     2281 cagtattaac tgcctgcaac accacgcgtg gcgttggtga agacatttct gatggcggta
     2341 acgcgatttc tggcgcagca acgaaagcgc agcaatagca ataagcaata acggtacgac
     2401 aggtatgtcg tgccgtttgt tttttctgcg atagtcacta aggtaatagt tgaaattccc
     2461 ctgccacctg gcaaaatatc cgttcaacca tcagctttgc aggacgacct gcaaacgcct
     2521 cttttcaccg gggacggccc caattctccg gagcctgata tgtcctggat tatcttagtt
     2581 attgctggtc tgctggaagt ggtatgggcc gttggcctga aatataccca cggctttagt
     2641 cgtttgacgc cgagtgttat taccgtgacg gcgatgattg tcagtatggc gctacttgcc
     2701 tgggcgatga aatcgttacc agtagggacg gcttatgccg tgtggacggg tattggcgca
     2761 gtcggcgcgg ccatcaccgg cattgtgctg ctcggtgagt ccgctaaccc gatgcgcctg
     2821 gcgagtctgg cgttaatcgt attggggatt attggtctga aactcagcac tcactaacta
     2881 ccgggctgct gtacccaaat aaacttactg acatcaaacc cttcccgggt cgcgactgcc
     2941 agcatctcct gtttcacttc gtcagaaatg gttggcgtgc gggagagtat ccacaggtag
     3001 tcgcggtccg gaccgcaaac cagcgcatgg cggtattccc gatcgagtgc aataacgtta
     3061 taaccgccat agaaaggacc aaagaatgac actttcagcg cagcgcgagt tggtgcgccg
     3121 gtaaagtacg ctttcccttc actctgctgc cacattcctc tgtcagggtt atagccttta
     3181 ttaatgacat tcaggccgcc gtcatcacgc aggctgtatg ttgcggtgac tttttccagt
     3241 ccacgttcaa aacggtgatc aaaacgggca atctcatacc aggtaccgag ataacgtttg
     3301 gcgtcgaaat tattcactac tgttacgcca cgcggcggcg taggagaact gcaggcaacg
     3361 accagaaatg cagctgtcgc tgcggcaacg agagggagca ggcgcataaa tgtttcctta
     3421 ctggtttttt tctaagtgta gatgacagca aggaaaagcg cagaaaaggt ccgaaaattc
     3481 ggacccgatg aaattttact gtagagcgtt gagaatctgc caggcggcgg cgactctcgc
     3541 tggattggga tagtttttgt tagccagcat cacgataccc agctcttttt ctggaataaa
     3601 cgcgacatag ctaccaaatc cgccggtcgc ccccgtttta tgcacccatg atgcgcgtac
     3661 tgcaggagtt gggggcgtaa tcgcttttac ggggcgtgct gccagtgcaa ttttattgtc
     3721 actgccgtta acgatgatgt caggatttac cggccagtcc agcatttccc agcccagacc
     3781 ctgatacata tcgccggttt gccagtagcg agattgtgcc agttgtatcc cttgttgaag
     3841 tgttttgtcg ttgatatcac ggtgattcat attgcttcgt acccagcagg ccatatcttc
     3901 aatggtcgac ttcacaccat aagtttcagc atctaacgcc cctggcgaaa catgaactgc
     3961 cttgccttcg cgatatcccc aggcgtaatt cttttcttct gcgggcggta cattaatcca
     4021 cgtatggttg agtttgagtg gctggaagac acgtgtttgc atcgcctgct caaaactcaa
     4081 accagacggc ttcacagcca gtgcgccgaa caaaccgata ctggagttgg catacagacg
     4141 ttgtgttcct ggcgcccatg caggctgcca gttttgatag aagcgcagca agtcgcttga
     4201 ggatttcacc tcatccggca cctgcaatgg caggccgcca gtagtgtagg ttgcgagatg
     4261 taatagtgtg atcccattcc actgtttagc ggtaagttca ggccagtatt ttgttgtggg
     4321 atcgcttaac ttgatttccc ctcgagcaat agcgtcgcca ccaagcacgc ccgtaaatgt
     4381 tttgctgacc gaacctaact caaacaacgt ttgctgtgtg acgggctgct ttttggcgat
     4441 gtccgcatag ccccaggtaa agtaataagg tttaccctga taaattaccg ccaccgccat
     4501 accggggatc ttttgttgct ctataagcgg ggtaattgtg cgatgcacaa tatcgttgat
     4561 ttgttgaggg gcagcaaatg tggagcaaga ggcggtaatt aataaggcgc agagcgtcgt
     4621 tttgaacata aggtctggtt tccatacaaa acggcccgcc ataggcgggc cggatttaca
     4681 ttggcgatac gttagattgt aacgatacca atcagcgtga caactgtcag gatagcagcc
     4741 agaccgtaga aaacccattt gcccgcaggc acgtgaattt tcagatcgtg catcgcgtgg
     4801 tgcatacggt gtaaaccaca ccacagcggc agaacgatca tcaggaacag gaatacgcga
     4861 ccaatgaagc tctgcgcgaa cgccagaacg cgctcgtagc tcagcgcatc acccggaaac
     4921 agccccagtg gcagcagaat acccaccagc aggatcatca ccggcgcaat aattgcgctc
     4981 cacataccac cggccccgaa gaggccccag aataccggtt cgtcagaacg ctttggattt
     5041 ggattaatca tctcaggctc cttaccagta cagggcaaca aacaggatta cgatggtggc
     5101 aaccacagtt accgcccaga gacttttgat aattggctct ggtcccattt tttcgtcttt
     5161 tacaatgata ttggctgctt tcggtgccag ttcaaaccag gttttggtat gcagcagagc
     5221 tgccgccaga gtgatcaggt taatgatcac gataaccggg ttttgtaaaa agtcgacgaa
     5281 tcccgcccag gcttccgggc catttttcag ggcaaacagc ccgaaaatca gttcaatgct
     5341 gaaccacaca gccggaaccg ccgtgccttc gcgcagcatg taaaagcgat aaaacggcaa
     5401 ttttttccac caggtggacg tcattggccg tacatacggt ttacgtttag tcgtcatgtt
     5461 gcactcctta gcgtggtttc agggtcgcga taagaaagtc tttcgaactt tctactttgc
     5521 cctgctgaat ggccgcagcc ggatcgacgt gtttcgggca gacttcggag caatagccca
     5581 cgaaagtaca gctccatacg ccgttctggc tgttcaactg cgccatacgc tccttcttac
     5641 cgtggtcgcg gctatcttcg ttataacgat gcgccagcgt aatggcagcc ggaccgatga
     5701 actctgggtt caggccaaac tgcgggcacg cggcgtagca cagaccacag ttgatgcaac
     5761 cggagaactg gtgatacttt gccatctgcg ccggggtctg gatgttagta ccctgatccg
     5821 cggtgcggga gttgccgatg atgtacggtt tgatcgcttc cagactttcg atgaagtggg
     5881 tcatatcgac caccagatcg cgttcaatcg ggaagttagc taacgcttca accttcatac
     5941 cgtcggtgta atcacgcagg aaggttttac atgccagttt tggcacgttg ttaaccatca
     6001 tgccgcagga accacaaatc gccatacggc aggaccagcg gtaactcagg tccggtgcca
     6061 ggttgtcttt gatgtagccc agcgcatcca gtaatgaggt agttgcgtca taaggcactt
     6121 catagaatgc gctatgcggt gcggtatcga cttccgggtt atagcgcacc acctcaattt
     6181 tcaggttttt catctcagcc attcgccttc tccttcttat tggctgcttc cgccttatcg
     6241 gctgcatccg cttcgccacc gtaaacgcgt ttagctggcg gcagcgtagt aatcttcacg
     6301 tcgctgtact ccaggcgagt cgtgccatca gcatcgcgga aggcgagggt gtgtttgagg
     6361 aagttgacgt cgtcacgctc ggtgcaacct tcgtccagac gctggtgtgc gccgcgggac
     6421 tctttacgtg ccattgcgga gtgcgccata cattcagcaa cgttcagacc gtggcccagt
     6481 tcaatggtgt agagcaggtc ggtgttgaac acgctggagg tgtcggtgat gcgcacgcgc
     6541 ttgaagcgtt cctgcagctc tgccagcttg tcgatggttt tctgcatcag ttccggcgta
     6601 cggtagatac cgcaaccttc ttccattgcc aggcccattt cgtcgcggat cttcgcccag
     6661 ttttcgccgc catcctggtt aaccagatct ttcagacgtt gttcaacgcc agctgcctgc
     6721 gcttcaattg ccgcttcgtt gccattaccg gcagttgctg cacgctctgt cgcttgttca
     6781 ccggccagac ggccgaagac caccagttcc gccagggagt tagaacccag acggtttgca
     6841 ccgtgcagac caacagagga acattcaccc acggcgaaca gacctttaat gcgggtttca
     6901 cagttctgat cggtttcgat accgcccatg gtgtagtgtg cggtcggacg taccggaatc
     6961 ggttctttaa ccggatcgac gccaacgtac gctttcgcca gttcgcagat gaacggcaga
     7021 cgttcatgca gttttttctc gccgaggtga cgcaagtcga gataaaccac atcgccacgc
     7081 ggcgtggaga tggtgttgcc tttacgccat tcgtgccaga aggcctgaga aactttgtcg
     7141 cgtggaccca gttccatata tttgtttttc ggctcgccca gcggagtttc cgggcccatg
     7201 ccgtaatctt gcagataacg gtagccattt ttgttgacca gaataccgcc ttcaccgcgg
     7261 cagccttcgg tcatcaggat accggaacct ggcagaccgg ttgggtgata ctgaacgaat
     7321 tccatgtcac gcagcggaac gccgtggctt agcgccatac ccataccgtc accggtaacg
     7381 atgccgccgt tggtgttgta acgataaacg cgacccgcac cgccagtagc cataacgacc
     7441 gcgttagcac ggatctgcac cagcgtgcct tccatcatgt tcattgctac caggccgcga
     7501 acatgaccat catcaaccag aatatccagc acgaaatgtt cgtcaaaacg ctggatctgc
     7561 gggaattgca gagaggtctg gaacagcgtg tgcagcatat ggaagccggt cttatcggcg
     7621 gcgaaccagg tgcgctcgat tttcatgccg ccgaagcgac gtacgttgac gctaccatcc
     7681 gggcgacggc tccatgggca tccccacagt tccagttggg tcatttcggt tgggcagtgg
     7741 tggacgaaat aatccacgac atcctgctca cacaaccagt cgccacccgc tactgtatcg
     7801 tgaaagtgat attcgaagct gtcatgatcc tgcgcgacag cggcggagcc cccttctgca
     7861 gcaacggtat ggctacgcat cgggtatact tttgagatta gtgcgatttt tgcattcgga
     7921 tttgcctgcg cggcagcaat tgcagcacgt aatcccgcgc caccggcgcc tacaatggca
     7981 agatcggctt gaaaggtttg cacgacattc ctccagattg tttttatccc acagccacgt
     8041 acttcagggt aagtacctga aagttacggt ctgcgaacgc tattccactg ctcctttata
     8101 ggtacaacag tatagtctga tggataagtc tgaaatttga cgagatcgat ttttttagtg
     8161 cgcaagaggg ctaaattatc actgaagatg attaatttaa ttactaaacc atcagatcgt
     8221 gcttttttta gtcactgccc accgctgttt gattcctgcg taaaatttgt ctcgccgtcg
     8281 cgttgcgagt agacttcgtg cccttgtcaa aaactggaga tttaactatg agcgaaacgg
     8341 catcctggca gccgagcgca tccattccta acttattaaa acgcgcggcg attatggcgg
     8401 agatccgtcg tttctttgcc gatcgtggag tgctggaggt ggagacgcct tgtatgagcc
     8461 aggcgacggt aaccgatatt catttggtcc cgtttgagac acgtttcgtt ggccccgggc
     8521 attcgcaggg gatgaatctc tggttaatga ccagcccgga ataccatatg aaacgtctgc
     8581 tggttgccgg ttgtgggccg gtattccagc tgtgccgcag cttccgtaat gaagagatgg
     8641 ggcgttatca caaccctgag ttcactatgc tggagtggta tcgaccgcac tatgatatgt
     8701 accggttgat gaacgaggtg gacgatctct tacaacaggt gctggactgc ccggcagcag
     8761 aaagcctttc ttatcaacaa gctttcttgc gttatctgga aattgaccca ctctctgccg
     8821 acaaaacgca actgcgggaa gtggcagcga aactggattt gagcaatgtt gctgataccg
     8881 aagaagaccg cgacacgttg ctacaattgc tgtttacctt tggcgtagag ccaaatattg
     8941 gtaaagaaaa accgaccttt gtgtaccact ttccagccag ccaggcatca ctggcgcaaa
     9001 tcagtaccga agatcatcgg gtcgctgaac gctttgaggt ttattataaa ggtattgagc
     9061 tggcgaatgg tttccatgaa ttgacggatg cccgtgagca gcaacaacgc tttgaacaag
     9121 ataaccgtaa gcgcgcggcg cgcggtttgc cgcagcaccc cattgaccag aatctgattg
     9181 acgccttgaa agtcggtatg cctgactgtt ccggcgtggc attaggtgtt gatcgtctgg
     9241 tgatgttggc gctgggcgcg gagacactgg ctgaagtcat cgcctttagc gttgaccggg
     9301 cataattctg aaaaattact gaattaacag cgaagaatgg cgtaataagc gccattctgt
     9361 agcatatgtt ttcatttttc cgctaaaggc cacaatttaa gactggatat ttgatatcat
     9421 ccaggtatca attctgtatt ggtttttacg ctgcctgctc aatgttgcgc agtgcagttc
     9481 agtgggcacg tgttatacac gcgctgaaat gaaggatggt ttcatgcctc acacgataaa
     9541 aaaaatgagt ctgataggac tcatattgat gatctttact tccgtatttg gttttgccaa
     9601 tagcccatcg gcttattact taatgggtta tagtgcgatt cccttttata tattttctgc
     9661 attgttattc tttattccat tcgccttaat gatggctgaa atgggagctg cttatcgcaa
     9721 agaagaaggt ggtatctatt cctggatgaa taatagtgtc ggaccacgtt ttgccttcat
     9781 tggtacgttt atgtggtttt cctcttatat catctggatg gtgagtacct ccgcgaaagt
     9841 ttgggtaccg ttctcaacat tcctctatgg tagcgacatg acccagcact ggcgtattgc
     9901 cggactggag cctacgcagg tggttggtct gctggcagtg gcatggatga ttctggtcac
     9961 cgtcgttgct tctaagggga ttaataaaat tgcccgcatt actgcggtgg gcggtattgc
    10021 agtaatgtgt ctgaatttag tattgctgtt agtaagcatt actattttgt tattaaatgg
    10081 tgggcatttc gcgcaggata ttaatttcct tgcatcaccg aacccgggtt atcagtccgg
    10141 tctggcaatg ctatcgtttg tggtatttgc catttttgcc tatggcggaa ttgaagcggt
    10201 tggtggtctg gtcgataaaa cggaaaatcc agaaaagaac tttgccaaag gtattgtttt
    10261 tgccgctatt gttatttcaa tcggttattc gctggcaata tttttatggg gcgtcagcac
    10321 aaactggcag caggtattaa gtaatggttc cgttaacctc ggcaatatta cctatgtgct
    10381 gatgaagagc cttgggatga cgctgggtaa tgcactgcat ttgtcacctg aagcgtcatt
    10441 gtcgctgggc gtatggtttg cgcgtattac tggactttcg atgttcctcg cctataccgg
    10501 tgcgttcttt acgctttgct attcaccgtt gaaagccatc atccagggga cgccgaaagc
    10561 attgtggccg gaaccgatga cgcgcctgaa tgcgatgggg atgccgtcta tcgccatgtg
    10621 gatgcagtgc gggttggtta ctatcttcat tctgctggtt tcgtttggtg gcggtaccgc
    10681 atcggcgttc tttaacaagc tgacgctgat ggcgaacgtg tctatgacgc ttccttacct
    10741 gttcctcgcg ctggctttcc cattctttaa agcacgtcag gatctcgaca gaccatttgt
    10801 gattttcaaa acgcgtatgt cggcaatgat cgcgacggtg gttgtcgtac tggtggtgac
    10861 atttgcgaac gtcttcacca tcattcaacc tgtggttgaa gctggagact gggacagcac
    10921 attgtggatg attggcggcc ctgtcttctt ctcgctgtta gcgatggcga tttaccagaa
    10981 ctattgcagt cgcatggcga ataaacctga gttagctctc gactgatcat atgaaatcct
    11041 gcaactcatc gtctctccgg cgatgagttg tcaggaagta atgcgtatgg atgatactct
    11101 ccagggccgg tgataactga agatgatgtt aagctgctaa atgagccacg ttgacatcat
    11161 ttggcagctt catgcattac aaacttcccg cctgacgacc tttgcccgca gaagttaatg
    11221 ttctccccgt ttgtttacca ttcaggcttt ccagacgcat ctggaagggc gggaatggca
    11281 tatcgatacc gtgggcatgg aagcctgcca gaatcagctg gtggatctca tggcgtagcg
    11341 gcatacggtg acccatctca gcggcgtaaa tacgcagctc gaaaatctga atcccttgtt
    11401 gcagatccac caggaagact tccggtgccg ggttgtcgat caccagcgag cagcgacgcg
    11461 ctgcggtgag caggatttcc gtcacttctt cactgttggc atcggcaggg gccggaatcg
    11521 tcaacaccac gcgcgtgacc gagtcagaga gcgaccagtt gataaactgc tcggtaataa
    11581 acgccttgtt cggcacgatt atctctttac ggtcccagtc gctgatggtg gttgcgcggg
    11641 tattaatttt cgtcacgcta ccagtaagat cgcggatcgt taccgtatcg ccaatgcgaa
    11701 tcggtttttc gaacaggatg atcaggccag agataaagtt ggcgaaaatt tcctgtaaac
    11761 caaaaccgag accaacaccg agcgcggcaa ccagccactg caatttcgac cactcaatac
    11821 caatcattga gaagccgacc agcccgccaa tcagcatcag cagatatttg gtgatggtgg
    11881 tgatggcgta acccgtaccc ggcgttaaat ccaggtgctg caaaatcgcc agttccagca
    11941 gcgcgggcag gttgcgcacc agctgcgtgg tgatgataaa caccagaatg gcaatcagca
    12001 ccgcaccgag ggtaattggc tccagacttt ctacgccctg taccgtggag gtgacatccc
    12061 acagcgaaat attttcgagg aagccgaaag cggaatggat ttctgaccac agcacaatga
    12121 ccgaaagcag ggcgatcaac atcaaaattg agcgcaccag ccgcaaggat tgcgcactga
    12181 tggcatcgag atcgacttcg ctttcatcga cttcaattgc cccttccggg ctactgtgat
    12241 gatgtgcttc ctcttcgcca cgcgcacgtt gcgctaacat ctctgcccgg cgatgcttcg
    12301 cccggtcaaa cgccagcctg cggcgttgga tcagcatcca gcggcggata acgtgataaa
    12361 ccaccagtag caggaaccag atggcaaccg aggtttcaag cctcgctaac agcgcctgtg
    12421 ccgttgccag ataacccacc gccgacgcca gaatggcaac cagtggcgcg ccaatcatca
    12481 tgttccacag catatggttg gtaatgttgt cgccgctgcc ctctttgttg agatacagtg
    12541 ggatcccggc ctttttcagg ctgagggtga ccaccgccag cgcaccgcaa atgaggataa
    12601 agcaaagccg tcccagcgaa ccggagaatt cacggtcgtc gaggttatcg aacatcatca
    12661 gcgccataat cagcggcaca ataagcccga tgctcatcag gtagtagcgc atcccacggg
    12721 aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg cgggcaaagg
    12781 tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca tcgccaatcg
    12841 cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc atccacagca
    12901 ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc agccagaagt
    12961 gatcctgagt cactttgcca actttcgccg ccgaacgttc aagaaaacgg gtgaaatagc
    13021 ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc aaaattgtct
    13081 ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg tccagcgaaa
    13141 tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc gggcgcacgt
    13201 cagaggtcca gaacagatag cggtgcgttg cttcgttcac ctctttcagc gcatcctcca
    13261 gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg ccaccctgca
    13321 acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata cggttttgct
    13381 cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc tgtttattaa
    13441 gcagatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta tcaagctgtt
    13501 gtggttttgg catttctggc agccgtgcca cctgcgcccg cagcgcttcg ccgagcagat
    13561 tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc cggacctgta
    13621 acgtctggct ggcagcctga cgctgttgcg aggcaacgag atccatccgc tgcgcctgtt
    13681 gattcaaagc cgccgatagt tcgcggttaa ttttgaattg cgcgacgata tctttcggca
    13741 aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc gcttcaagct
    13801 gacgttggct gttcaactga ttacgtaagg cctgcaaata cgcatccagt tgctggctct
    13861 ctttttccgc cagctctgag cgtaagcgcg ctaattcctg acggttattg gcagacagct
    13921 gcgccagttc cagttcatca acgagcgctt tcaggcgtgc ggagtcagac tgcaacgcga
    13981 aattttgtgc ctgattgagc ggggtattgc cagtaagcgt tcccaggcgg cgctcgatct
    14041 catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc gaatcggcaa
    14101 tctcgcgggc gcgctcctgc tcttgctggg cctgacggct tttatccagc aactggctgc
    14161 tgacctggag aatttcctga ttcagcgcgt cggtagacat ccccggcgac acgctgcgcg
    14221 gttcgtcacg catgttgttt aattgtgcgc gcagagtagc ggaaagtttc gggtaattat
    14281 caatgacttc ttgatattgc ttgatgcgct caagggaacc ttttcgttcc tcaagcgcat
    14341 ttaaggcgga ctggagcgcc tctacgactt ccggctgcgc gggtttcgcc gcttttgcct
    14401 gctccagttc ttgtgagatt tgtttgctat cgggggccgt cgcggcgtac gccccccaac
    14461 tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt cctttgatgg
    14521 attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct gcttcgatct
    14581 cttcagcagg aagcggggcc ggttcagcgt ctggcgtaac aaaggtttcg gtagatactg
    14641 ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc agattcactt
    14701 tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc atttcctggc
    14761 ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag cgcttgatga
    14821 taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg ccaacaatcg
    14881 tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg caaatcacgc
    14941 gttcgttacg ggcaaacaga ttcggcacgt tctgagccgt gagatggtta acggagaaga
    15001 gatcgcccgg tacgtagatc atctcacgca gaataccgtt gcacggcatg tgtacgcgat
    15061 ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac aggtccgcca
    15121 tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg gcttgcagga
    15181 ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc atgaccagta
    15241 cattcggatc ggtatcgatt gggcgtactt cgccacgcag cggacggaca aagaattcgt
    15301 taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg accttgtagt
    15361 atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg cttgcgcccc
    15421 aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa agtttaaatg
    15481 aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc ctacatgccg
    15541 gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag tcatccgtat
    15601 cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg tgatagtttt
    15661 cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg cagcccggat
    15721 cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg aattcgacaa
    15781 agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc acaccagggg
    15841 aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc gtggtgtgct
    15901 gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc agccccagca
    15961 acgcattcag caggctggat ttgccgacgc cagactgccc ggcaaaaatg ctgatgcgcc
    16021 cggtcaacgc ctcttccagc ggttttagcc catcctgagt atggctggaa accatcaata
    16081 cgcgataacc gatattgcgg tagatatcca tctgctcgtt gacgaacgcc atgccttcgt
    16141 cgtccagcag gtctatcttg ttgagcacaa taatcggctc aatctgcaag gtttcgcagg
    16201 ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg gagacaatga
    16261 caatctggtc gatgttggcg gcaataggtt ttacgccgtc gtagaaatcc ggacgcgtca
    16321 acaccgaggt acgctcatgc accgcttcca cgatcccttt gacatttacg ccttccgccg
    16381 ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg atggtacggc
    16441 gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc ataccaaagc
    16501 ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag tcgggcttct
    16561 ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc tggcctttgg
    16621 agagtttatt tttactcaat cgtacagact cctggtcgcc cctgatgggc aaaacatcta
    16681 tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat agcatgagtg
    16741 ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat cccgagcgcg
    16801 atcgcattat tgagattgcc acgctggtga ccgatgccaa cctgaatatt ctggcagaag
    16861 ggccgaccat tgcagtacac cagtctgatg aacagctggc gctgatggat gactggaacg
    16921 tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg atgggcgatc
    16981 gggaagctga actggcaacg ctcgaatttt taaaacagtg ggtgcctgcg ggaaaatcgc
    17041 cgatttgcgg taacagcatc ggtcaggacc gtcgtttcct gtttaaatac atgccggagc
    17101 tggaagccta cttccactac cgttatctcg atgtcagcac cctgaaagag ctggcgcgcc
    17161 gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag gcgatggatg
    17221 atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc aagctgtaaa
    17281 atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt tgacgctaaa
    17341 ttgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa acttttttgg
    17401 ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg cagaaatgcg
    17461 aaaattacga aagcaaaatt aagtagtacg cgggaatagc tcagttggta gagcacgacc
    17521 ttgccaaggt cggg
//
LOCUS       DAPGWD010000087        17445 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPGWD010000087 DAPGWD010000000
VERSION     DAPGWD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.088"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(101..832)
                     /gene="rlmB"
                     /locus_tag="RHX88_002818"
     CDS             complement(101..832)
                     /gene="rlmB"
                     /locus_tag="RHX88_002818"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDV2231487.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1012..3453)
                     /gene="rnr"
                     /locus_tag="RHX88_002819"
     CDS             complement(1012..3453)
                     /gene="rnr"
                     /locus_tag="RHX88_002819"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDV2231488.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3492..3917)
                     /gene="nsrR"
                     /locus_tag="RHX88_002820"
     CDS             complement(3492..3917)
                     /gene="nsrR"
                     /locus_tag="RHX88_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDV2231489.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4122..5420)
                     /gene="purA"
                     /locus_tag="RHX88_002821"
     CDS             complement(4122..5420)
                     /gene="purA"
                     /locus_tag="RHX88_002821"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDV2231490.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5524..5721)
                     /locus_tag="RHX88_002822"
     CDS             complement(5524..5721)
                     /locus_tag="RHX88_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDV2231491.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5803..6807)
                     /gene="hflC"
                     /locus_tag="RHX88_002823"
     CDS             complement(5803..6807)
                     /gene="hflC"
                     /locus_tag="RHX88_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDV2231492.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6810..8069)
                     /gene="hflK"
                     /locus_tag="RHX88_002824"
     CDS             complement(6810..8069)
                     /gene="hflK"
                     /locus_tag="RHX88_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDV2231493.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8155..9435)
                     /gene="hflX"
                     /locus_tag="RHX88_002825"
     CDS             complement(8155..9435)
                     /gene="hflX"
                     /locus_tag="RHX88_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDV2231494.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9511..9819)
                     /gene="hfq"
                     /locus_tag="RHX88_002826"
     CDS             complement(9511..9819)
                     /gene="hfq"
                     /locus_tag="RHX88_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDV2231495.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9905..10855)
                     /gene="miaA"
                     /locus_tag="RHX88_002827"
     CDS             complement(9905..10855)
                     /gene="miaA"
                     /locus_tag="RHX88_002827"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDV2231496.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10848..12695)
                     /gene="mutL"
                     /locus_tag="RHX88_002828"
     CDS             complement(10848..12695)
                     /gene="mutL"
                     /locus_tag="RHX88_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDV2231497.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12705..14042)
                     /gene="amiB"
                     /locus_tag="RHX88_002829"
     CDS             complement(12705..14042)
                     /gene="amiB"
                     /locus_tag="RHX88_002829"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDV2231498.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14061..14522)
                     /gene="tsaE"
                     /locus_tag="RHX88_002830"
     CDS             complement(14061..14522)
                     /gene="tsaE"
                     /locus_tag="RHX88_002830"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDV2231499.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14494..16041)
                     /gene="nnr"
                     /locus_tag="RHX88_002831"
     CDS             complement(14494..16041)
                     /gene="nnr"
                     /locus_tag="RHX88_002831"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDV2231500.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16040..17179
                     /gene="queG"
                     /locus_tag="RHX88_002832"
     CDS             16040..17179
                     /gene="queG"
                     /locus_tag="RHX88_002832"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDV2231501.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17162..17215)
                     /locus_tag="RHX88_002833"
     CDS             complement(17162..17215)
                     /locus_tag="RHX88_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231502.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3970 a         4962 c         4376 g         4137 t
ORIGIN      
        1 aatattaata aaatgaatat ttgcagtacg taattatctt accagctata gacaaaaaaa
       61 aaccatccaa atctggatgg cttttcataa ttcagacgaa ctagctgcgc tgacgaaccg
      121 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      181 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      241 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      301 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      361 caaggttagt cacccgaatc agtggaacgc tttctgccgc gccgcaggct acttttttcg
      421 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcgtga acgccagcag
      481 cgtccgcgct acgcaggcac gcgccgaggt tgtgcggatc ggttacaccg tcgaggatca
      541 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      601 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      661 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      721 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      781 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      841 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      901 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      961 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
     1021 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1081 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1141 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac gacgcttgcc
     1201 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1261 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1321 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1381 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1441 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1501 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1561 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1621 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1681 cccctgctct ttcgccagca gatacttaat ggcgcggtgc agcgtcaggt caggataacg
     1741 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc aggccaaagt gaccacggtt
     1801 ttcaggatcg taaatcgcct gtttcatcga gcgcagcagc atggtttgca gcatttctgc
     1861 atctggacga tcggcaaccg actccagcag ctccgcgtag tcacgcggtt ccggcttatt
     1921 accgcccggc agttccagcc ccagctccgc cagaacagaa cggaaagagg taatcgcttc
     1981 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttggctttct caacaaaacg
     2041 cgccgccgag atattcgcca gaatcatgca ctcttcaatc aacttgtgtg cgtcgttacg
     2101 ctgagtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2161 gctctcaaat gagatcccgc cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2221 atgcaactct tcgagatgct taaccagtgg ggcgtactgc tcgcgcaaat cctgatcgcc
     2281 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2341 gtagaatttg tagccagtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2401 caggcggtct acctgcgggt tgagcgagca caggccgtta gagagcactt ccggcagcat
     2461 cgggataacc tgcgaaggga agtacaccga cgtaccacgg ttacgcgctt ctctgtccag
     2521 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2581 gccgccgcgt tttttctcgc agtaaaccgc atcgtcaaag tcacgggcgt cttcgccatc
     2641 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2701 ttcttctttc agcccggcaa cctgttgctc aacagcctgc ggccagatgt acggaatttc
     2761 atgggtacgc agagcgatat caaccgccat gccggtgccc atattatcgc caaggacttc
     2821 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2881 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2941 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     3001 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3061 agcgcccagc ggctgagcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3121 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3181 gccaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3241 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3301 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3361 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3421 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3481 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3541 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3601 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3661 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccga tttacccagg
     3721 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3781 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3841 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3901 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3961 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     4021 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4081 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacggatcg
     4141 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacacca
     4201 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcagacc gctacgatct
     4261 ttcacgccga aggtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4321 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4381 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4441 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4501 cgacgacgac gccccgtagt tgcgccgaat tcgttaccct gcttgcagag gaactcgcca
     4561 gtttcatcaa acagttcggt cgggaacgga cctgcaccta cacgagtgga gtaagctttg
     4621 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4681 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4741 gtaccctgcg caccttcaaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4801 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4861 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4921 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4981 agaccgcgac gtgctacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     5041 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg
     5101 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5161 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5221 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5281 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5341 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5401 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5461 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
     5521 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5581 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5641 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5701 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5761 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5821 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5881 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5941 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcatgat
     6001 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6061 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6121 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6181 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6241 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatccgctgc
     6301 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6361 gagggtcaga cgaccacggg aatcggtgac gatgtctttc acgtccaggc gaccaatttc
     6421 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6481 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagaatc
     6541 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6601 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagacccgg
     6661 ctcataaacc agaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6721 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6781 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6841 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6901 gctccactgg ttgtggagga agaggctggc ggcaaacgca gcagattgct ggcaccgtta
     6961 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     7021 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7081 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7141 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7201 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac
     7261 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7321 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7381 cccatgtcat acggacggat agtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7441 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataacccc gcgcagggcg
     7501 ctgtcggtag cctgacgcag actgtcatcc gggctggtca cgctatacag atatttttcc
     7561 ggattggtaa cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7621 aacatcacac cggaagcggc cagttcacgc acggcttcca cgtttaccgg tttgacttcg
     7681 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7741 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7801 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7861 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7921 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7981 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     8041 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8101 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8161 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8221 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8281 ctgataaaaa cgacttctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8341 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8401 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8461 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8521 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgta catccgccgc
     8581 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8641 tgcaaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcca acacggtttc
     8701 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8761 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8821 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8881 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8941 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     9001 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9061 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9121 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9181 gctcagggca tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat
     9241 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9301 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9361 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9421 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9481 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9541 ggaagtattc tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9601 gttactgtga tgagaaaccg gacgagacgg gacaacagta gaaatcgcgt gcttgtaaac
     9661 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9721 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9781 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9841 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9901 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9961 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
    10021 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10081 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10141 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10201 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10261 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10321 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10381 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10441 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10501 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10561 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10621 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10681 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aagggcagaa tcaacgctta
    10741 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10801 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10861 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10921 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10981 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    11041 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11101 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11161 atacccaatt ccgccagggc agactgcgct ttttctaatg ccgatttttc ttcggcagaa
    11221 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11281 aattgtgcct gacgtagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11341 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11401 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11461 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11521 ttcggccagg gggcagccgg acgactgcct gatgctggcg caggagtgta gcgcggagct
    11581 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11641 ggaatggaac gcggtgcagg ttggggttca tcgtccagcg gtagcggcgt ttccagttgc
    11701 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11761 aaacgcactt cgtgtttggc ggggtgcacg ttgacgtcca cctgatgcgg atcgatctcc
    11821 agatacagca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11881 atcgcgtgat tgatcaggcg atcgcgcatc atgcgaccgt tcacgtagca atactgaatt
    11941 tctgccagtg cgggcgtggt gtgatttgga tcggccaccc agccgcgtag cgtgagatcg
    12001 ccgtgttgcc attcaatcgc cagcgcttgt tcaagaaaag cggtgccgca aatcgcgcct
    12061 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12121 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatctca
    12181 tcaatgtggt taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12241 tagaacagat ccagcacctc cagcgtcgtc cccacaggat gcgccgccgg ttttaccgtc
    12301 acgttcatat cgcgcccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12361 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12421 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgcgcc
    12481 agcgccagcg ccagctcgtc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12541 ttcgccccac cgcgttcgat atcaatatcg atacgtgtcg cacctgcatc gaggctgttt
    12601 tccaccagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12661 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12721 cgatctggcg tcgtcaccgt actggccgtt tgtgccgttg ccccctgcgg cgcagattgc
    12781 atcggatgcg caaggaaata attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12841 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12901 accagtactg atgggatatc cggcgaacgc agaacgccga gactcgcgtg ttctggtcgg
    12961 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    13021 cctacccgtt gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13081 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgactg tttctcgtgc
    13141 tgctccagcc agctggccat ttcgctgttg gcgcgacggt tagagagcac ccatacggaa
    13201 gcgccagtcg cactgcggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13261 ttttgcttac gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13321 gttaaaacgc ccttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13381 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13441 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13501 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13561 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13621 ttcgcaacca cgggcggcgg aggaggaggt ggcggcgcat cggcgttaat cgtaaagacg
    13681 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13741 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13801 agattattgc cgctgaacaa caacggcagt ccctgaatca cgcccgtttg tttgatatcg
    13861 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13921 aacgttatcc gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13981 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    14041 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14101 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14161 tccgggtcag gaagaacacc agtaccttgt tgtggccact ccaccaggca gatggcatcg
    14221 ttggcaaaat aatcgcgaat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14281 aaatcaaagt gataaaccat taaattgtcg agcgtatagg gttcgaccag cgtataagtg
    14341 gggcttttga cattaccctg atgacccaga gcttgtaaaa agccccggct aaaggtggtt
    14401 ttacctgcgc ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct
    14461 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14521 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14581 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgcag caccgtgcgc
    14641 gacacagccc gcacaggcgg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14701 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14761 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14821 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14881 gacggaacag cctaacaacc gtgcggcctc gccaggatgc ggcgtgatca cgcgattgtg
    14941 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    15001 gcgaaaattc tcaacttttt gcagtgcttt tttcccccac tcttgctggc ccagaccggg
    15061 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tcgtcagttc
    15121 atgcaccatc aattccggtc gtgcagtcag cagcggcgca atgttttcac tgcgggtcag
    15181 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15241 cgccgtgccg tgatcgccac cgataattac cagccgcccg tgatcgcctt tatgcgaagt
    15301 cgggcgacgc ggttttagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15361 ctcctgacct gccagccaac tatccagccc cagtgagtca aaatgcagtt gtccggtaac
    15421 atcccgcgct tttccagtga gcaagcctgg tttcagcgca ataaaagtga tggtgtgatc
    15481 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15541 aaccgccgca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15601 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15661 gggccagaca atattcgaag catggatctc gcctcccgcg tttaaccatg cttcgcgtgc
    15721 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccagcagcg tgacctcaat
    15781 gccgaccgct gtggccagcc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15841 gcacagcacc agccaatggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15901 ttcgccagcg cgaagcatca gctcatagag tgtaagcccc agcgcatctg ccgcctcacg
    15961 ttctccgcgg cggatatcgt cggcgtgcca gacggtgtgt ggtatactta cggggttttt
    16021 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    16081 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    16141 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16201 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattgcccg gtacgctgcg
    16261 cgtgatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16321 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16381 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16441 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16501 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttctt tcttcttttt
    16561 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16621 taaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16681 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcga tcccggaaga
    16741 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    16801 gtggaatcgc tattcacaac tcaccacaga agaggatttc agcccgcgta agccgctaca
    16861 cgcaccggaa ctcattgagt tattcgcctg gagcgaagag aagtttttaa aagtcacgga
    16921 aggatcggcg attcgtcgta ttggtcacct gcgttggctg cgtaatatcg ccgtagcatt
    16981 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    17041 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    17101 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    17161 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17221 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt aatttttaac
    17281 ttatctatca ataggttaaa aaaatactat tcaccgtgcg aagtttcttt gaaattaaga
    17341 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacgaaaacc
    17401 agcaataacg catcgctgtg gatattttat tgagagaaga atttg
//
LOCUS       DAPGWD010000088        17417 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPGWD010000088 DAPGWD010000000
VERSION     DAPGWD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.089"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(43..1326)
                     /gene="gltA"
                     /locus_tag="RHX88_002834"
     CDS             complement(43..1326)
                     /gene="gltA"
                     /locus_tag="RHX88_002834"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDV2231503.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1532..1639)
                     /locus_tag="RHX88_002835"
     CDS             complement(1532..1639)
                     /locus_tag="RHX88_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231504.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2020..2424
                     /gene="sdhC"
                     /locus_tag="RHX88_002836"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2020..2424
                     /gene="sdhC"
                     /locus_tag="RHX88_002836"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV2231505.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2418..2765
                     /gene="sdhD"
                     /locus_tag="RHX88_002837"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2418..2765
                     /gene="sdhD"
                     /locus_tag="RHX88_002837"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDV2231506.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2765..4531
                     /gene="sdhA"
                     /locus_tag="RHX88_002838"
     CDS             2765..4531
                     /gene="sdhA"
                     /locus_tag="RHX88_002838"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDV2231507.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4547..5263
                     /gene="sdhB"
                     /locus_tag="RHX88_002839"
     CDS             4547..5263
                     /gene="sdhB"
                     /locus_tag="RHX88_002839"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDV2231508.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5464..8265
                     /gene="sucA"
                     /locus_tag="RHX88_002840"
     CDS             5464..8265
                     /gene="sucA"
                     /locus_tag="RHX88_002840"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDV2231509.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8280..9497
                     /gene="odhB"
                     /locus_tag="RHX88_002841"
     CDS             8280..9497
                     /gene="odhB"
                     /locus_tag="RHX88_002841"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDV2231510.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9591..10757
                     /gene="sucC"
                     /locus_tag="RHX88_002842"
     CDS             9591..10757
                     /gene="sucC"
                     /locus_tag="RHX88_002842"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDV2231511.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10757..11626
                     /gene="sucD"
                     /locus_tag="RHX88_002843"
     CDS             10757..11626
                     /gene="sucD"
                     /locus_tag="RHX88_002843"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDV2231512.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11730..12452)
                     /gene="mngR"
                     /locus_tag="RHX88_002844"
     CDS             complement(11730..12452)
                     /gene="mngR"
                     /locus_tag="RHX88_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDV2231513.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12621..14537
                     /gene="mngA"
                     /locus_tag="RHX88_002845"
     CDS             12621..14537
                     /gene="mngA"
                     /locus_tag="RHX88_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDV2231514.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14555..17188
                     /gene="mngB"
                     /locus_tag="RHX88_002846"
     CDS             14555..17188
                     /gene="mngB"
                     /locus_tag="RHX88_002846"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDV2231515.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNENGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         4212 a         4345 c         4738 g         4122 t
ORIGIN      
        1 ttgaacggcg ggttaaaata tttacaactt agcaatcaac cattaacgct tgatatcgct
       61 tttaaagtcg cgtttttcat atcctgtata cagctgacgc ggacgggcaa tcttcatacc
      121 gtcactgtgc atttcgctcc agtgggcgat ccagccaacg gtacgtgcca ttgcgaaaat
      181 gacggtgaac atggaagacg gaatacccat cgctttcagg atgataccag agtagaaatc
      241 gacgttcggg tacagtttct tctcgataaa gtacgggtcg ttcagcgcga tgttttccag
      301 ctccatagcc acttccagca ggtcatcttt ggtgcccagc tctttcagaa cttcatggca
      361 ggtttcacgc attacggtgg cgcgcgggtc gtaatttttg tacacgcggt gaccgaagcc
      421 catcaggcgg aaagaatcat ttttatcttt cgcacgacga acaaattccg gaatgtgttt
      481 aacggagcta atttcttcca gcattttcag cgccgcttcg ttagcaccac cgtgcgcagg
      541 tccccacagt gaagcaatac ctgctgcgat acaggcaaac gggttcgcac ccgaagagcc
      601 agcggtacgc acggtggagg tagaggcgtt ctgttcatgg tcagcgtgca ggatcagaat
      661 acggtccata gcacgttcca gaatcggatt aacttcatac ggttcgcacg gcgtggagaa
      721 catcatattc aggaagttac cggcatagga gagatcgttg cgcggataaa caaatggctg
      781 accaatggaa tacttgtaac acatcgcggc catggtcggc attttcgaca gcaggcggaa
      841 cgcggcaatt tcacgatgac gaggattgtt aacatccagc gagtcgtgat agaacgccgc
      901 cagcgcgccg gtaataccac acatgactgc cattggatgt gagtcgcgac ggaaagcgtg
      961 gaacagacgg gtaatctgct cgtggatcat ggtatgacgg gtcaccgtag ttttaaattc
     1021 gtcatactgt tcctgagtcg gtttttcacc attcagcagg atgtaacaaa cttccaggta
     1081 gttagaatcg gtcgccagct gatcgatcgg gaaaccgcgg tgcagcaaaa taccttcatc
     1141 accatcaata aaagtaattt tagattcgca ggatgcggtt gaagtgaagc ctgggtcaaa
     1201 ggtgaacaca ccttttgaac cgagagtacg gatatcaata acatcttgac ccagcgtgcc
     1261 tttcagcaca tccagttcaa cagctgtgtc cccgttgagg gtgagttttg cttttgtatc
     1321 agccatttaa ggtctcctta gcgccttatt acgtaagact gccggaactt aaatttgcct
     1381 tcgcacatca acctggcttt acccgtttgt tatttggctc gccgctctgt gaaagagggg
     1441 aaaacctggg tacagagctc tgggcgcttg caggtaaagg atccattgat gacgaataaa
     1501 tggcgaatca agtacttagc aatccgaatt attaaacttg tctaccacta ataactgtcc
     1561 cgaatgaatt ggtcaatact tccacactgt tacataagtt aatcttaggt gaaataccga
     1621 cttcataact tttacgcatt atatgctttt cctggtaatg tttgtaacaa ctttgttgaa
     1681 tgattgtcaa attagatgat taaaaattaa ataaatgttg ttatcgtgac ctggatcact
     1741 gttcaggata aaacccgaca aactatatgt aggttaattg taatgatttt gtgaacagcc
     1801 tatactgccg ccaggtctcc ggaacaccct gcaatcccga gccacccagc gttgtaacgt
     1861 gtcgttttcg catctggaag cagtgttttg catgacgcgc agttatagaa aggacgctgt
     1921 ctgacccgca agcagaccgg aggaaggaaa tcccgacgtc tccaggtaac agaaagttaa
     1981 cctctgtgcc cgtagtcccc agggaataat aagaacagca tgtgggcgtt attcatgata
     2041 agaaatgtga aaaaacaaag acctgttaat ctggacctac agaccatccg gttccccatc
     2101 acggcgatag cgtccattct ccatcgcgtt tccggtgtga tcacctttgt tgcagtgggc
     2161 atcctgctgt ggcttctggg taccagcctc tcttcccctg aaggtttcga gcaagcttcc
     2221 gcgattatgg gcagcttctt cgtcaaattt atcatgtggg gcatccttac cgctctggcg
     2281 tatcacgtcg tcgtaggtat tcgccacatg atgatggatt ttggctatct ggaagaaaca
     2341 ttcgaagcgg gtaaacgctc cgccaaaatc tcctttgtta ttactgtcgt gctttcactt
     2401 ctcgcaggag tcctcgtatg gtaagcaacg cctccgcatt aggacgcaat ggcgtacatg
     2461 atttcatcct cgttcgcgct accgctatcg tcctgacgct ctacatcatt tatatggtcg
     2521 gttttttcgc caccagtggc gaactgacat atgaagtctg gatcggtttc ttcgcctctg
     2581 cgttcaccaa agtgttcacc ctgctggcgc tgttttctat cttgattcat gcctggatcg
     2641 gcatgtggca ggtgttgacc gactacgtta aaccgctggc attgcgcctg atgctgcaac
     2701 tggtgattgt cgttgcactg gtggtttacg tgatttatgg attcgttgtg gtgtggggtg
     2761 tgtgatgaaa ttgccagtca gagaatttga tgcagttgtg attggtgccg gtggcgcagg
     2821 tatgcgcgcg gcgctgcaaa tttcccagag cggccagacc tgtgcgctgc tctctaaagt
     2881 cttcccgacc cgttcccata ccgtttctgc gcaaggcggc attaccgttg cgctgggtaa
     2941 tacccatgaa gataactggg aatggcatat gtacgacacc gtaaaagggt cggactatat
     3001 cggtgaccag gacgcgattg aatatatgtg taaaaccggg ccggaagcga ttctggaact
     3061 cgaacacatg ggcctgccgt tctcgcgtct tgatgatggt cgtatctatc aacgtccgtt
     3121 tggcggtcag tcgaaaaact tcggcggcga gcaggcggca cgcactgcgg cggcggctga
     3181 ccgtaccggt cacgcactgt tgcacacgct ttatcagcag aacctgaaaa accacaccac
     3241 cattttctcc gagtggtatg cgctggatct ggtgaaaaac caggatggcg cggtggtggg
     3301 ttgtaccgca ctgtgcatcg aaaccggtga agtggtttat ttcaaagccc gcgctaccgt
     3361 gctggcgact ggcggggcag ggcgtattta tcagtccacc actaacgccc acattaacac
     3421 tggcgacggc gtcggcatgg ctatccgcgc aggcgtaccg gtgcaggata tggaaatgtg
     3481 gcagttccac ccgaccggta ttgccggtgc gggcgtactg gtcaccgaag gttgccgtgg
     3541 tgaaggcggt tatctgctga acaaacatgg cgaacgcttt atggagcgtt atgcgccgaa
     3601 cgccaaagac ctggcgggcc gtgacgtggt ggcgcgttcc atcatgattg aaatccgtga
     3661 aggtcgcggc tgtgatggtc cgtgggggcc acatgcgaaa ctgaaactcg accacctggg
     3721 taaagaagtt ctcgaatccc gtctgccggg tattctggaa ctctcccgta ccttcgctca
     3781 cgttgatccg gtgaaagagc cgattccggt tatcccaacc tgtcactaca tgatgggtgg
     3841 tattccgacc aaagtgaccg gtcaggcgct gacggtgaac gagaaaggcg aagatgtggt
     3901 tgttccgggg ctgtttgccg ttggtgaaat cgcttgtgta tcggtacacg gcgctaaccg
     3961 tctgggcggc aactcgctgc tggacctggt ggtctttggt cgcgcagcgg gtctgcatct
     4021 gcaagagtct atcgccgagc agggcgcatt gcgcgatgcc agcgagtctg atgttgaagc
     4081 atctctggat cgtctgaacc gctggaacaa taatcgtaac ggtgaagatc cggtggcgat
     4141 tcgtaaagcg ctgcaagaat gtatgcagca taacttctcg gtcttccgtg aaggtgatgc
     4201 gatggcgaaa gggcttgagc agttgaaagt tatccgcgag cgtctgaaaa atgcccgtct
     4261 ggatgacact tccagcgagt tcaacaccca gcgcgttgag tgcctggaac tggataacct
     4321 gatggaaacg gcgtatgcaa cggctgtttc tgccaacttc cgtaccgaaa gccgtggcgc
     4381 gcatagccgc ttcgacttcc cggatcgtga tgatgaaaac tggctgtgcc actccctgta
     4441 tctgccagag tcggaatcca tgacgcgccg aagcgtcaac atggaaccga aactgcgccc
     4501 ggcattcccg ccgaagattc gtacttacta atgcggagac aggaaaatga gactcgagtt
     4561 ttcaatttat cgctataacc cggatgttga tgatgctccg cgtatgcagg attacaccct
     4621 ggaagcggaa gaaggtcgcg acatgatgtt gctggatgcg cttatccagc tgaaagagaa
     4681 agatcccagc ctgtcgttcc gccgctcctg ccgtgaaggt gtgtgcggtt ccgacggtct
     4741 gaacatgaac ggcaagaatg gtctggcctg tattaccccg atttcggcac tcaaccagcc
     4801 gggcaagaag attgtgattc gcccgctgcc aggtttaccg gtgatccgcg atttggtggt
     4861 agacatggga caattctatg cgcaatatga gaaaattaag ccttacctgt tgaataatgg
     4921 acaaaatccg ccagctcgcg agcatttaca gatgccagag cagcgcgaaa aactcgacgg
     4981 gctgtatgaa tgtattctct gcgcatgttg ttcaacctct tgtccgtctt tctggtggaa
     5041 tcccgataag tttatcggcc cggcaggctt gttagcggca tatcgtttcc tgatcgatag
     5101 ccgtgatacc gagactgaca gccgcctcga cggtttgagc gatgcattca gtgtattccg
     5161 ctgtcacagc atcatgaact gcgtcagtgt atgtccgaag gggctgaacc cgacgcgcgc
     5221 catcggccat atcaagtcga tgttgttgca acgtaatgcg taattaaatg ttgatgccgg
     5281 ggaaatcccc cggctattca ggaaatcttt aaaaactgcc cctgacacta agacagtttt
     5341 taaaggttcc ttcgcgagcc actacataga caagagctcg caagtgaacc ccggcacgca
     5401 catcactgtg cgtggtagta tccacggcga agtaagcata aaaaagatgc ttaagggatc
     5461 acgatgcaga acagcgcttt gaaagcctgg ttggactctt cttacctctc tggcgcaaac
     5521 cagagctgga tagaacagct ctatgaagac ttcttaaccg atcctgactc ggttgacgct
     5581 aactggcgtt cgacgttcca gcagttacct ggtacgggag tcaaaccgga tcaattccac
     5641 tctcaaacgc gtgaatattt ccgccgcctg gcgaaagacg cttcacgtta ctcttcaacg
     5701 atctccgacc ctgacaccaa tgtgaagcag gttaaagtcc tgcagctcat taacgcatac
     5761 cgcttccgtg gtcaccagca tgcgaatctc gatccgctgg gactgtggca gcaagataaa
     5821 gtggccgatc tggatccgtc tttccacgat ctgaccgaag cagacttcca ggaaaccttc
     5881 aacgtcggtt catttgccag cggcaaagaa accatgaaac tcggcgagct gctggaagcc
     5941 ctcaagcaaa cctactgtgg cccgattggt gccgagtata tgcacattac cagcaccgaa
     6001 gaaaaacgct ggatccaaca gcgtatcgag tctggtcgcg cgactttcaa tagcgaagag
     6061 aaaaaacgct tcttaagcga actgaccgcc gctgaaggcc ttgaacgtta cctcggcgca
     6121 aaattccctg gcgcaaaacg cttctcgctg gaaggcggtg acgcgttaat cccgatgctt
     6181 aaagagatga tccgccacgc tggcaacagc ggcacccgcg aagtggttct tgggatggcg
     6241 caccgtggtc gtctgaacgt gctggtgaac gtgctgggta aaaaaccgca agacttgttc
     6301 gacgagtttg ccggtaaaca taaagaacac ctcggcacgg gtgacgtgaa ataccacatg
     6361 ggcttctcgt ctgacttcca gaccgatggc ggcctggtgc acctggcgct ggcgtttaac
     6421 ccgtctcacc ttgagattgt aagcccggtc gttatcggct ctgttcgtgc ccgtctggac
     6481 agacttgatg agccgagcag caacaaagtg ctgccaatta ccatccacgg tgacgccgca
     6541 gtgaccggac agggcgtggt tcaggaaacc ctgaacatgt cgaaagcgcg tggttatgaa
     6601 gttggcggta cggtacgtat cgttatcaac aaccaggttg gtttcaccac ctctaatccg
     6661 ctggatgccc gttctacgcc gtactgtact gatatcggca agatggttca ggcaccgatt
     6721 ttccacgtta acgcggacga tccggaagcc gttgcctttg tgacccgtct ggcgctcgat
     6781 ttccgtaaca cctttaaacg tgatgtcttc atcgacctgg tatgctaccg ccgtcacggc
     6841 cacaacgaag ccgacgagcc gagcgcaacc cagccgctga tgtatcagaa aatcaaaaaa
     6901 catccgacac cgcgcaaaat ctacgctgac aagctggagc aggaaaaagt ggcgacgctg
     6961 gaagatgcca ccgagatggt taacctgtac cgcgatgcgc tggatgctgg cgattgcgtg
     7021 gtagcagagt ggcgtccgat gaacatgcac tctttcacct ggtcgccgta cctcaaccac
     7081 gaatgggacg aagagtaccc gaacaaagtt gagatgaagc gcctgcagga actggctaaa
     7141 cgcatcagca cggtgccgga agcggttgaa atgcagtctc gcgttgccaa gatttatggc
     7201 gatcgccagg cgatggctgc cggtgagaaa ctgttcgact ggggcggcgc ggaaaacctc
     7261 gcttacgcca cgctggttga cgaaggcatt ccggttcgcc tgtcgggtga agactccggt
     7321 cgcggtacct tcttccaccg ccacgcggtg atccacaacc agtctaacgg ttccacttac
     7381 acgccgttgc aacacatcca taacgggcag ggcgcgttcc gcgtctggga ctccgtactg
     7441 tctgaagaag cagtgctggc gtttgaatac ggttatgcca ccgcagaacc acgcactctg
     7501 accatctggg aagcgcagtt cggtgacttc gccaacggtg cgcaggtggt tatcgaccag
     7561 ttcatctcct ctggcgagca gaaatggggc cggatgtgtg gtctggtgat gttgctgccg
     7621 cacggttacg aagggcaggg gccggagcac tcctccgcgc gtctggaacg ttatctgcaa
     7681 ctttgtgctg agcaaaacat gcaggtgtgc gtaccgtcta ccccggcaca ggtttaccac
     7741 atgctgcgtc gtcaggcgct gcgcgggatg cgtcgtccgc tggtcgtgat gtcgccgaaa
     7801 tccctgctgc gtcatccgct ggcggtttcc agcctcgaag aactggcgaa cggcaccttc
     7861 ctgccagcca tcggtgaaat cgacgagctt gatccgaagg gcgtgaagcg cgtagtaatg
     7921 tgttctggta aggtttatta cgacctgctg gaacagcgtc gtaagaacaa tcaacacgat
     7981 gtcgccattg tgcgtatcga gcaactctac ccgttcccgc ataaagcgat gcaggaagtg
     8041 ttgcagcagt ttgctcacgt caaggatttt gtctggtgcc aggaagagcc gctcaaccag
     8101 ggcgcatggt actgcagcca gcatcatttc cgtgaagtga ttccgtttgg ggcttctctg
     8161 cgttatgcag gccgcccggc ctccgcctct ccggcggtag ggtatatgtc cgttcaccag
     8221 aaacagcagc aagatctggt taatgacgcg ctgaacgtcg aataaataaa ggatacacaa
     8281 tgagtagcgt agatattctg gtccctgacc tgcctgaatc cgtagccgat gccaccgtcg
     8341 caacctggca taaaaaaccc ggcgacgcag tcgtacgtga tgaagtgctg gtagaaatcg
     8401 aaactgacaa agtggtactg gaagtaccgg catcagcaga cggcattctg gatgcggttc
     8461 tggaagatga aggtacaacg gtaacgtctc gtcagatcct tggtcgcctg cgtgaaggca
     8521 acagcgccgg taaagaaacc agcgccaaat ctgaagagaa agcgtccact ccggcgcaac
     8581 gccagcaggc gtctctggaa gagcaaaaca acgatgcgtt aagcccggcg atccgtcgcc
     8641 tgctggctga acacaatctc gacgccagcg ccattaaagg caccggtgtg ggtggtcgtc
     8701 tgactcgtga agatgtggaa aaacatctgg cgaaagcccc ggcgaaagag tctgctccgg
     8761 cagcggctga tccggcggcg caaccggctc tggctgcacg tagtgaaaaa cgtgtcccga
     8821 tgactcgcct gcgtaagcgt gtggcagagc gtctgctgga agcgaaaaac tccaccgcca
     8881 tgctgaccac gttcaacgaa gtcaacatga agccgattat ggatctgcgt aagcagtacg
     8941 gtgaagcgtt tgaaaaacgc cacggcatcc gtctgggctt tatgtccttc tacgtgaaag
     9001 cggtggttga agccctgaaa cgttacccgg aagtgaacgc gtctatcgac ggcgatgacg
     9061 tggtttacca caactatttc gacgtcagca tggcggtttc tacgccgcgc ggcctggtga
     9121 caccggtact gcgtgatgtc gataccctcg gcatggcaga catcgagaag aaaatcaaag
     9181 agctggcagt caaaggccgt gacggcaagc tgaccgttga agatctgacc ggtggtaact
     9241 tcaccatcac caacggtggt gtgttcggtt ccctgatgtc tacgccgatc atcaacccgc
     9301 cgcagagcgc aattctgggt atgcacgcta tcaaagatcg tccgatggcg gtgaatggtc
     9361 aggttgagat cctgccgatg atgtacctgg cgctgtccta cgatcaccgt ctgatcgatg
     9421 gtcgcgaatc cgtgggcttc ctggtaacga tcaaagagtt gctggaagat ccgacgcgtc
     9481 tgctgctgga cgtgtagtag tttaagcttc acctgcactg tagaccgaca tggatagggt
     9541 gtcggtctac ggtttaaaag ataacgatta ctgaaggatg gacagaacac atgaacttac
     9601 atgaatatca ggcaaaacaa ctttttgccc gctatggctt accagcaccg gtgggttatg
     9661 cctgtactac tccgcgcgaa gcagaagaag ccgcttcaaa aatcggtgcc ggcccgtggg
     9721 tagtgaaatg tcaggttcac gctggtggcc gcggtaaagc gggcggtgtg aaagttgtaa
     9781 acagcaaaga agacatccgt gcttttgcag aaaactggct cggcaagcgt ctggtaacgt
     9841 atcaaacaga tgccaatggc caaccggtta accagattct ggttgaagca gcgaccgata
     9901 tcgctaaaga gctgtatctc ggtgccgttg tagaccgtag ttcccgtcgt gtggtcttta
     9961 tggcctccac cgaaggcggc gtggaaatcg aaaaagtggc ggaagaaact ccgcacctga
    10021 tccacaaagt tgcgcttgat ccgctgactg gcccgatgcc gtatcaggga cgcgagctgg
    10081 cgttcaaact gggtctggaa ggtaaactgg ttcagcagtt caccaaaatc ttcatgggcc
    10141 tggcgaccat tttcctggag cgcgacctgg cgttgatcga aatcaacccg ctggtcatca
    10201 ccaaacaggg cgatctgatt tgcctcgacg gcaaactggg cgctgacggc aacgcactgt
    10261 tccgccagcc tgatctgcgc gaaatgcgtg accagtcgca ggaagatccg cgtgaagcac
    10321 aggctgcaca gtgggaactg aactacgttg cgctggacgg taacatcggt tgtatggtta
    10381 acggcgcagg tctggcgatg ggtacgatgg acatcgttaa actgcacggc ggcgaaccgg
    10441 ctaacttcct tgacgttggc ggcggcgcaa ccaaagaacg tgtaaccgaa gcgttcaaaa
    10501 tcatcctctc tgacgacaaa gtgaaagccg ttctggttaa catcttcggc ggtatcgttc
    10561 gttgcgacct gatcgctgac ggtatcatcg gcgcggtagc agaagtgggt gttaacgtac
    10621 cggtcgtggt acgtctggaa ggtaacaacg ccgaactcgg cgcgaagaaa ctggctgaca
    10681 gcggcctgaa tattattgca gcaaaaggtc tgacggatgc agctcagcag gttgttgccg
    10741 cagtggaggg gaaataatgt ccattttaat tgataaaaac accaaggtta tctgccaggg
    10801 ctttactggt agccagggga ctttccactc agaacaggcc attgcatacg gcactaaaat
    10861 ggttggcggc gtaaccccag gtaaaggcgg tactacccac ctcggcctgc cggtgttcaa
    10921 caccgtgcgt gaagctgttg ctgctactgg cgctaccgct tctgttatct acgtaccagc
    10981 accgttctgc aaagactcca ttctggaagc tattgatgca ggcatcaaac tgattatcac
    11041 catcactgaa ggcatcccga cgctggatat gctgactgtg aaagtgaaac tggacgaagc
    11101 cggcgttcgt atgatcggcc cgaactgccc gggcgttatc actccgggtg aatgcaaaat
    11161 cggtatccag ccgggtcaca ttcacaaacc gggtaaagtg ggtatcgttt cccgttccgg
    11221 tacactgacc tatgaagcgg ttaaacagac cacggattac ggtttcggtc agtcgacctg
    11281 tgttggtatc ggcggtgacc cgatccctgg ctctaacttt atcgacattc tcgaaatgtt
    11341 cgaaaaagat ccgcagaccg aagcgatcgt aatgatcggt gagatcggcg gtagcgctga
    11401 agaagaagcg gcagcgtaca tcaaagagca tgttaccaag ccagttgtgg gttacatcgc
    11461 tggtgtgact gcgccgaaag gcaaacgtat gggccacgcg ggtgccatca ttgccggtgg
    11521 gaaagggact gcggatgaga aattcgctgc tctggaagcc gcaggcgtga aaaccgttcg
    11581 cagcctggcg gatatcggtg aagcactgaa aactgttctg aaataaatat ctgtaataag
    11641 aaatagccct cgccgcttcc ctctacagga atggcgaagg gctgtcggtt tcgacatggt
    11701 tggccatcgt atgatggcct tttttgtgct tatcgcgatg attttcgttg cgctatcagg
    11761 gtaaatttat agtcatcggt attaaaagcg ttgcggctat attcaaacac ccgaccatca
    11821 actaaatatc cacgcgatac tttttcaaga atcggctttg tctggctgat attaagcaga
    11881 cggctcatct cttcggttgg catcagagga atgatttcct gttcgctacg atcgataacc
    11941 attttcttca cttcttcaat aaagtgatat tttgaatttt ccatgacctg ccaggtgaga
    12001 tccgggaaca acgcaagcgg catccaggtt tcttccagcg ccattggctt ttgcttgcga
    12061 tagcgcacgc gcttcacatg ccacacacga tcctgcgcgg tgatttgtag ctgttgctga
    12121 agaaaatcgt cagccggaat cacttcaaat atcaggactt cactgtgtgt atcgacgtga
    12181 cggtccgaca gtttttcatc aaaactggtt aactgaaaaa tatcgtaatt gacccgctct
    12241 tctttgacgt aagtcccgct gccctgaatg ctttcgagga tctgttgctc gactaactgg
    12301 cgcaaagcct gacgcaccgt aacccggctg acgccaaact ctgtttgtaa tgccgattcg
    12361 gtaggtaacg catcgccagg tttaagctcg ccacgcgcaa tttgttcacg aatgcgatcg
    12421 gcaatctgcc ggtataaggg cttgtgtccc atttttagta tctcattaat acgaatttag
    12481 ccattatgcc cgataaattc atcctgtaaa taatacaaat acaatacaaa taatttcaat
    12541 caagtgaaat tgatcacata atggtattgt tttatcgggc acactggcgc gactataaaa
    12601 acgatcaagt gaggatcatg atgaatctga cgactctgac ccaccgcgat gcgttgtgtc
    12661 tgaatgcgcg ctttaccagc cgtgaagagg ccatccacgc gttgactcaa cgtcttgctg
    12721 ctctggggaa aatttccagt actgagcaat ttctggaaga agtgtatcgc cgtgaaagcc
    12781 ttggcccgac cgccttaggt gaagggttgg ctgtgccgca tggcaaaact gctgcggtaa
    12841 aagaagcggc gtttgcggtc gccacactca gcgagccgct tcagtgggaa ggcgttgatg
    12901 gcccggaagc ggttgattta gtggtgctgc tggcgattcc ccccaatgaa gcgggtacta
    12961 cgcatatgca actgctgaca gcgctgacca cgcgcctggc ggatgatgag attcgggcgc
    13021 gtatacagtc ggcgacgacg cctgatgagt tgctctcggc gctggatgac aagggaggca
    13081 cgcaaccttc tgcctctttt tccaacgcgc caactatcgt ctgcgtaacg gcctgtccgg
    13141 cgggtattgc tcacacctat atggctgcgg aatatctgga aaaagccgga cgcaaactcg
    13201 gcgtaaatgt ttacgttgaa aaacaaggcg ctaacggcat tgaagggcgt ttaacggcgg
    13261 atcaactcaa tagtgcaacc gcctgtattt ttgcggctga agtcgccatc aaggagagtg
    13321 agcgttttaa tggcattccc gcgctttcag tgcctgttgc cgagccgatt cgccatgcag
    13381 aagcgttgat ccaacaagcg cttaccctca agcgtagcga tgagacgcgt accgtacagc
    13441 aagatacgca accggtgaaa agtgtcaaaa cggagctgaa acaggcactg ttgagcggaa
    13501 tctcttttgc cgtaccgttg attgtcgcgg ggggcacggt gctggcggtc gcggtattac
    13561 tgtcgcaaat cttcgggcta caagatctgt ttaatgaaga aaactcctgg ctgtggatgt
    13621 accgcaagct gggcggcggg ctgctcggaa ttttgatggt accggtgctc gcggcctata
    13681 ccgcctattc tctggcagat aaaccggcgt tagcgccagg ctttgcggct ggacttgccg
    13741 ccaacatgat cggctccggg tttctcggcg cggtcgttgg cggattgata gccggttact
    13801 tgatgcgctg ggtgaaaaat cacttgcgtc ttagcagtaa attcaatgga ttcctgactt
    13861 tttatctcta cccggtgctc ggtacgttgg gagcgggcag tctgatgctg tttgtggtgg
    13921 gagaacctgt cgcctggatc aataactcgc ttaccgcctg gctgaacggt ctgtcaggaa
    13981 gtaacgcgct gttgctgggt gccattctcg gttttatgtg ttcctttgac cttggagggc
    14041 cagtgaacaa agccgcttat gcattctgcc tgggcgcaat ggcgaacggc gtttacggcc
    14101 cgtatgccat tttcgcctcc gtcaaaatgg tttcggcatt taccgtaacc gcttccacga
    14161 tgctcgcacc gcgcctgttt aaagagtttg aaattgagac cgggaaatcc acctggctgt
    14221 tagggctggc aggtattacc gaaggggcga tcccgatggc gattgaagat ccgctgcggg
    14281 ttattggttc gtttgtgctg ggctctatgg taacgggcgc tattgtcggt gcgatgaata
    14341 tcggcctttc gacacccggt gccggcattt tctcgctctt tttacttcat gataatggcg
    14401 cgggcggtgt tatggcggca attggctggt ttggtgcggc attggtgggg gctgcaatct
    14461 cgactgcaat tctcctgatc tggcggcgtc acgcggttaa gcatggcaac tatctgactg
    14521 atggcgtaat gccataaaca aaaaggaaac gacgatgaaa gcggtatctc gcgttcacat
    14581 caccccgcat atgcattggg atcgggagtg gtatttcacc accgaagagt cacgcattct
    14641 gctggtcaat aatatggaag agatcctgtg ccgactggaa caggacaacg aatacaaata
    14701 ttacgtactc gacgggcaaa cggcgatcct cgaagattat ttcgcggtga aaccggaaaa
    14761 caaagaccgc gtgaagaaac aggtagaagc cggcaagttg attatcggcc cctggtatac
    14821 ccagaccgat accacgattg tttctgcgga atccatcgtc cgtaatctga tgtacggaat
    14881 gcgtgactgc ctcgcgtttg gcgagccgat gaaaataggt tatttaccag attcctttgg
    14941 catgtccggg caactgccgc atatctacaa tggatttggc attacccgca ccatgttctg
    15001 gcgcggatgt tcggagcgcc acggtactga taaaaccgag tttttgtggc aaagcagtga
    15061 cggtagcgaa gtgacggcgc aggtgctgcc gctgggctac gccatcggta agtacttacc
    15121 tgccgacgaa aacggattac gtaaacgcct cgacagttat tttgacgtgc tggaaaaagc
    15181 gtctgtaacc aaagagattt tgctgccgaa tgggcatgac cagatgccat tgcagcaaaa
    15241 tatcttcgaa gtgatggata agctacgtga gatctaccct caacgtaagt ttgtgatgag
    15301 ccgctttgaa gaggtatttg agaagatcga agcgcagcgt gaaagtctgg ccaccctgaa
    15361 aggtgaattt atcgatggca aatatatgcg cgtacatcgc accatcggtt ccacgcgcat
    15421 ggatatcaaa attgcccacg cacgtattga aaataagatt gttaatctgc tggaaccgct
    15481 ggcaacgctg gcctggacgt tgggttttga ataccaccac ggcttgctgg agaaaatgtg
    15541 gaaagagatc ttaaaaaatc atgcccacga cagtatcggt tgttgttgca gtgacaaagt
    15601 tcatcgcgaa attgtcgccc gcttcgaact ggctgaagac atggcggata atctgctgcg
    15661 tttctacatg cgcaaaattg ccgataacat gccacagagt gatgccgaca aactcgtgct
    15721 gtttaacctg atgccctggc cgcgggaaga agttattaac accactgtgc gcctgcgcgc
    15781 cagccagttt aatctacgag acgatcgcgg tcagcctgta ccgtatttta ttcgccatgc
    15841 ccgtgagatc gatccaggcc taatcgatcg gcaaatagtt cattacggta attacgatcc
    15901 ctttatggag tttgatatac agatcaacca gattgtccct tctatgggct atcgcacgct
    15961 ttatatcgaa gcgaatcagc ctggcaacgt gattgcggca aaaagtgacg ctgaagggat
    16021 actggaaaat gctttctggc aaattgcgct caatgagaat ggaacgctac aactggtaga
    16081 taaagatagc ggtgtgcgct atgatcgggt gttgcaaatt gaagaaagct ctgatgatgg
    16141 tgatgaatat gactattcac ccgcaaaaga agagtgggta atgacttctg caacagcgaa
    16201 accgcaatgc gagattactc atgaggcctg gcagagcagg gctgttatcc gctatgacat
    16261 ggcggtgccg ctcaatttgt tggaacgaag cgtccggcaa tccacgggca gagtaggggt
    16321 ggagatggtt gtcactctga gtcataacag cagacgtatt gatgtggata tcaaccttga
    16381 taaccaggct gacgatcatc gccttcgcgt cctgatccct acatctttta acaccgatag
    16441 tgttctggca gatacccagt ttggttcgct aacgcgcccc gtgaacgaca gtgcaatgaa
    16501 caactggcag caagaaggct ggaaagaagc gccggttccg gtatggaata tgctcaacta
    16561 tgcagcctta caggaagggc gtaacggcat ggctgtcttt agcgaagggc tacgtgaatt
    16621 tgaagtcatc ggtgaagaga agaaaacctt tgccattacg ttgctgcgtg gcgtgggctt
    16681 actgggcaaa gaagatctgt ttttaaggcc tgggcggcct tcgggaatta aaatgccagt
    16741 cccggactca caactacgtg gtctgctttc ttgtcgccta agtttattga gttataccgg
    16801 tacgccaacc gccgctggtg tagctcagca ggcgcgagca tggctgactc cagtacagtg
    16861 ttacaacaaa atcccatggg atgcgatgaa gctcaacaaa gccggattca acgtgccgga
    16921 aagttatagt ttgttgaaaa tgcccccagt gggatgcctg ataagcgcac ttaagaaagc
    16981 tgaagaccga caagaagtga ttttacggct gtttaatccg gctgaatcag caacctgtga
    17041 tgcgactgtt gctttcagtc gcgaggtgat ttcttgctca gaaacgatga tggatgaaca
    17101 cattaccacc gaggaaaatc aaggttcaaa tctatcgggg ccttttttac ccggccagtc
    17161 acggacgttc agttaccggc ttgcctgaat agcaatcaaa ccgaagccac atatgcgcgg
    17221 ccagattgtt gacaaagggc gctttgttca tgccggatac ggcatgaacg ctttattcgg
    17281 tctacaaaag caggcaaatt caatatattg cagagatgat gtagacactg gcaagcgtag
    17341 cgcatcaggt aattttgcgt ttatcttcac tctcaagcca cgtatatgtg gctttatttt
    17401 taacaaaata ataaccc
//
LOCUS       DAPGWD010000089        17056 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPGWD010000089 DAPGWD010000000
VERSION     DAPGWD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.090"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(60..866)
                     /gene="trpA"
                     /locus_tag="RHX88_002847"
     CDS             complement(60..866)
                     /gene="trpA"
                     /locus_tag="RHX88_002847"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDV2231516.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(866..2059)
                     /gene="trpB"
                     /locus_tag="RHX88_002848"
     CDS             complement(866..2059)
                     /gene="trpB"
                     /locus_tag="RHX88_002848"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDV2231517.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2071..3432)
                     /gene="trpCF"
                     /locus_tag="RHX88_002849"
     CDS             complement(2071..3432)
                     /gene="trpCF"
                     /locus_tag="RHX88_002849"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDV2231518.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3433..5028)
                     /gene="trpD"
                     /locus_tag="RHX88_002850"
     CDS             complement(3433..5028)
                     /gene="trpD"
                     /locus_tag="RHX88_002850"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDV2231519.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5028..6590)
                     /gene="trpE"
                     /locus_tag="RHX88_002851"
     CDS             complement(5028..6590)
                     /gene="trpE"
                     /locus_tag="RHX88_002851"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDV2231520.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6612..6705)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6682..6726)
                     /gene="trpL"
                     /locus_tag="RHX88_002852"
     CDS             complement(6682..6726)
                     /gene="trpL"
                     /locus_tag="RHX88_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDV2231521.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6864..7745
                     /gene="yciV"
                     /locus_tag="RHX88_002853"
     CDS             6864..7745
                     /gene="yciV"
                     /locus_tag="RHX88_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDV2231522.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7742..8362
                     /locus_tag="RHX88_002854"
     CDS             7742..8362
                     /locus_tag="RHX88_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDV2231523.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8463..9335
                     /gene="rluB"
                     /locus_tag="RHX88_002855"
     CDS             8463..9335
                     /gene="rluB"
                     /locus_tag="RHX88_002855"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDV2231524.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9375..9965)
                     /gene="cobO"
                     /locus_tag="RHX88_002856"
     CDS             complement(9375..9965)
                     /gene="cobO"
                     /locus_tag="RHX88_002856"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDV2231525.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(9962..10720)
                     /locus_tag="RHX88_002857"
     CDS             complement(9962..10720)
                     /locus_tag="RHX88_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDV2231526.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            10940..11989
                     /gene="sohB"
                     /locus_tag="RHX88_002858"
     CDS             10940..11989
                     /gene="sohB"
                     /locus_tag="RHX88_002858"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDV2231527.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12025..12276)
                     /locus_tag="RHX88_002859"
     CDS             complement(12025..12276)
                     /locus_tag="RHX88_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDV2231528.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12656..15253
                     /gene="topA"
                     /locus_tag="RHX88_002860"
     CDS             12656..15253
                     /gene="topA"
                     /locus_tag="RHX88_002860"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDV2231529.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            15463..16437
                     /gene="cysB"
                     /locus_tag="RHX88_002861"
     CDS             15463..16437
                     /gene="cysB"
                     /locus_tag="RHX88_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDV2231530.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            16732..16896
                     /locus_tag="RHX88_002862"
     CDS             16732..16896
                     /locus_tag="RHX88_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDV2231531.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            16899..>17056
                     /locus_tag="RHX88_002863"
     CDS             16899..>17056
                     /locus_tag="RHX88_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231532.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQP"
BASE COUNT         4173 a         4305 c         4501 g         4077 t
ORIGIN      
        1 tttttccggc ttcattaaag aaagttaaaa tgccgccagc ggaactggcg gctgtgggat
       61 taactgcgcg tcgccgcttt catcggttgt acaaaaactt tcagtgccgc cagcattttc
      121 tctggctcat taatatgttg ctcgatgatt ttaacaatgg ccgaaccaga aatcgcgccc
      181 gcagctcctg catcaatcgc tgcttttacc tgatccgggg cggaaatacc aaatccctgc
      241 aatggaggtg cagcgttgta ctctttcagc ttcgcaacca gatgattgag gggtaacgcg
      301 gcgcggtttt ctgcgccggt cacgcctgct cgtgacagca aataggtgta accacgaccg
      361 taagaggcta tctggcgcag caggtcgtca tcggcatttg gcgggcagat gaagataggt
      421 gcgacattat ggcgcaacgc ggcctggcgg aagggcgcgg actcttcaat tggcacatcg
      481 gcaaccagca ccgaatcgac gccgactttc tcgcactggg cataaaactc atcaatgcct
      541 ttgttaaaca ccagattggc atacatcaac aggccaatgg gaatggtcgg gtgtttctgg
      601 cgaatcagtg ccagcatttc aaaacattgt gccggagtca cacctgccgc aaaggcgcgc
      661 agagtggcgt tttgaatggt cgggccatcc gccagtgggt cggagaaggg gatacctaac
      721 tccagcgcgt cagcaccggc ttcaattagc gtatcgataa ttttcaatga ctgctcaatg
      781 cccggatcac cgagcgtgac gaaaggaacg aatgcgcctt ctttgcgctc cttcaactgg
      841 gcaaacagag attcgtagcg ttccatcaga tttcccctcg tgctttcaaa atatcgtgaa
      901 cggtgaagat gtctttatcg ccgcgaccgg aaaggttaac caccagtagc tgctctttat
      961 ccgggttttc gcgcatcatt ttcaacgcat gggccagggc gtgggaggat tccagcgccg
     1021 ggatgatccc ttcgtgcagg cacagcgttt tgaaggcttc aagggcttca tcatcggtaa
     1081 tagacacgta atcagcgcgt ccagtgctgt taagatacgc gtgttgtggg ccgacagacg
     1141 ggaaatccag tccggcggag atggagtaag attcttcaat ctgcccgtct tcggtttgca
     1201 tcatcggcgc tttcataccg aaatagatac ccacgcgacc atgttttagc ggtgcgccgt
     1261 gctcgccagt ttcgataccg tgaccacctg gctccacacc aatcaggccg acgttggttt
     1321 cattgatgaa atcagcaaac atgccgatgg cattcgaacc gccgccaaca caggcgataa
     1381 cggcatccgg caggcgacct tctctttcca gaatctgcgc tttggtttct tcgccaatca
     1441 tccgctgaaa ctcacgcaca atggtcggat aaggatgcgg gccagctgcg gtgcccagca
     1501 tatagtgcgc ggtttcgtaa ctaccggacc agtcgcgcag cgcctcgtta caggcatctt
     1561 tcagcgtcgc ggaaccgcta tgcaccggga tcacttccgc acccattaag cgcatacgaa
     1621 aaacgttagg cgactggcgt tcaacgtctt tggcacccat ataaatacgg catttcaggc
     1681 cgagcagggc gctggcaagg gccgacgcca cgccatgctg accggcaccg gtttcggcga
     1741 tgatttcggt tttacccatc cgcttcgcca gcaacgcctg ccccagcacc tggttagttt
     1801 tatgcgcgcc gccgtgcagc aaatcttcac gcttgagata cagcgtggtg ttcgtcccgg
     1861 ctgtaatgtt ctggcatttg gtcagcgcgg ttggacgccc ggcatagttt ttcagcaggt
     1921 cgttgaactg agcctgaaat tcaggatctt tttgcgcact gacaaaagct tcttccagct
     1981 ggcgcagagc aggcatcagg atttgtggca cgtacatgcc gccaaactca ccaaaatagg
     2041 ggttaagtaa tgttgtcatt gttcctttcc ttaatatgcg cgcagcgtct ggaaaaccga
     2101 ggccaaaaga cgtgcgtctt tgatgcccgg ttgcgactct acagcagaat taaaatcaag
     2161 tccggcgcag ccggtttgtg ccgcttccac gcagttatct gcgcctaagc cccccgccag
     2221 cagaacgttg ccaagcgatt gaccatttaa tagtgaccag tcaaaacgtt gcccgcttcc
     2281 accctggccg ttgtctaaaa catatttatc aacgtgctga aactcgcggg cgggcagggt
     2341 ttcaccgacg cttaatgctt tccagatggc aacatgtgct ggcagagctt cacgcagcgt
     2401 atcgatatac agctgttctt cattaccatg cagttgcact gccgccagcg ataacacctt
     2461 agctttgtcc accacatcgg caatatcgtg attgcggaac acgccaacat actgcaacgg
     2521 tgccgcagcc atcacttcct gcgcctgttc aacgttgacg caacgcggtg atgtcgcaac
     2581 aaaaatcaac ccaccgtaaa tcgcgcccgc gtcataagct gctttagcat cttgcccacg
     2641 cgtcaggcca catactttat tctcacccag caacacccgg cgcacggcgg cgtgcaaatc
     2701 gtcatgggcc atcaacgccg aaccaatcag aaaaccgtta gcgaagtggc ttaactcgcg
     2761 cacctgagcg taagtattga tgccggattc gctgattacc gtcacgttgt gccccagttt
     2821 cggcgcaagc tcgcgggtac ggttgagatc aatcgacaaa tcacgcagat cgcggttgtt
     2881 gatgccaacg acctttgccc ccaatgcaat ggcacgctcc agttcctctt cattactgac
     2941 ttcggtcagc acacccatct ccagactgtg ggcgacggcg gcaagctggc gatattgttc
     3001 gtcatccagt actgaaagca ttaataagca ggcatcagcc tggtaatagc gcgccagata
     3061 gatctggtaa ggatcgataa tgaagtcttt acataaaatc ggctgcgggg cgatttggct
     3121 gacgatgggg aggaaatcaa agctcccctg aaaatatttc tcatcagtca gcaccgaaat
     3181 tgccgaagcg taatgtttat aaatggcggc aatgcgtgct ggatcgaaat catcacggat
     3241 cacgcctttt gacggcgacg ctttcttgca ctccagaata aacgccgtgc gcgcaccctg
     3301 tagcgcatca taaaaatgtc gcgtgctcgg ctgaacctca ttctgaaaac tggccagcgg
     3361 ttgctgctgt ttgcgggttt ctacccaaat cgccttgtct gcgacgattt tcgctaaaac
     3421 ggtttgcatc atttaccctc gtgccgccag tgcggtaact ctgtcgtaag cggaaccact
     3481 gcgcagtacc tcaagaacgg tttgcgcatt ggcttgcaga tcttcatggc catgcaggcg
     3541 cattaacatg gcgacgttcg cagcgacggc tgcttcatgg gcggcgtcgc ctttaccttg
     3601 taacaagcgt gttaaaatgt cacggttttc ttccggtgtt ccgcctgcca gttgctcctg
     3661 gtggtaggga gtcaggccaa aatcttcagc ggtcaattga tagctcttaa tttcgccgtc
     3721 atgcagctcg gcaacgattg tcggcgcgtg taatgaaact tcatccatcc cgccgctgtg
     3781 caccaccgcc gcgcgttgat accccagcac gcgcaaggtt tcggcaatcg gcagcaccag
     3841 ttccggacta taaacaccaa ttaacgccag cggcggatgc gccgggttaa tcaatggccc
     3901 cagcacattg aacagggtgc gggttttcag ttgctggcga accggcatcg cgtggcggaa
     3961 tccggtgtga tacttcggcg caaagaggaa acatacacct aactcatcca gcgcctggcg
     4021 cgatttatcg gcgttcatat caagattaat accgaacgcc gccagcagat cggacgaacc
     4081 agatttactg gagacgctac ggttgccgtg tttcgccact ttcagcccac aggccgcggc
     4141 gacaaacgca ctggcggtag aaatattgat actgttgctg ccgtcaccgc cagtaccgac
     4201 gatatcagca aacagataat ccgggcgcgg gaacggcgcg gcgttttcca gtagcgcggt
     4261 tgctgctccg gcgatctcgt tcgggtgctc accgcgaatt ttcatgctca ccagcgccgc
     4321 cgccagttgt tccggcttca gctcgccacg caccaccgct gaaaacagct ggtggctttc
     4381 ttgttggcta agcgtctgcg cctgatacag tttttccaga atcggttgca gcgtgttggc
     4441 tggctctagt ttctgctgcg cccaggccag cgtttgttcc agcaggcgag cgccctgggt
     4501 ggtgagaatg gattccggat ggaactggaa tccacaaacg cgatccgcat cgtgacgtac
     4561 tgccatcacc atgccattaa aatgggcgtt gatggttaaa ccggccggaa tgttactgcc
     4621 aaccagcgag tgataacgcg ccaccggcag cgggtttgtt aatccggcaa acatcgcctg
     4681 accgtcatgt tcaatgctcg acgctttacc gtgaagaatt tcgcccgcct gaccgacata
     4741 ccccccataa gcttcgacaa tcgcctgatg accgaggcaa atgccaataa tgggcagctt
     4801 gccacgcaag cgggtgagga gttccggcat acaaccggct tcgctcggca caccggggcc
     4861 aggagaaagc atcagtaccg gattgctcat cgtcgccagg cgttcaatta aggtctgcgc
     4921 cggaatatgg ttgcggtaaa tcaccacatt atgcccattg ctgcgcaact gatctgccag
     4981 gttgtacgta aaagagtcga tattatcgag cagcagaatg tcagccatca gaaagtctcc
     5041 tgtgcatgat gcgcggtggc aatagcgcgc agtacagcgc gggctttatt acgggtttcg
     5101 tcggcttccg actgcggaac agaatcaagg actacgccag caccggcttg cacggtggcg
     5161 ataccgtttt ccaccagcgc cgagcggatc acaatgcagg tgtcgagatc gccatgcgcg
     5221 gtgaaataac ctaccgcgcc gccgtagctg ccgcggcgac gaccttccgc ctcggcaatt
     5281 aactgcatgg cgcgcacttt cggcgcaccg cttaacgtcc ccatattcat acaggcgcgg
     5341 taagcgtgta gggcgtcgag atcgtggcgc agttcaccga ccacgcggga gaccaggtgc
     5401 atcacgtaag agtaacggtc aactttggtg aggtcggcga cgtagcggct gccgggggtg
     5461 caaatgcgtg ccagatcatt acgggcaaga tccaccagca tcagatgttc agaaagctct
     5521 ttatgatcgg tacgcatttc cagttcgatg cggctgtcga ggtctctgtc cagcgagcca
     5581 tcggcacgac gaccgcgtgg acgtgttccg gcaatcgggt agatctcaat ctggcggctg
     5641 gtggcgtcat acttgagcga actttccggc gacgcgccaa acagggtgaa atcattatcc
     5701 tgcataaaaa acatgtacgg gctgggatta ctctttttca gcacgtaata ggccgccagc
     5761 ggtgacgggc agggcagaga gaaacggcga gatggcacca cctggaaaat ttctccagcg
     5821 cgaatcgctt tttgcaacaa acgcactacg ccaccgaact cttcatcgct ctgattacat
     5881 tcacaacgca tatgcggcac ggaaaccacc ggcagcggcg gcgcggcttc ggtcagttgc
     5941 tgacgtagtt cgttcaggcg agcagtgaga cgttgttttt cttcttcatt cggagcaaac
     6001 aggctggcct gaatacgggt gctttttttc tgatggtcaa tcaccatcag agtttcagcg
     6061 agataaaaac agaaatcagg gcagttattt tccgctgaca gttgcggtaa atcttcaaat
     6121 cccgccacaa ggtcataaga gaacaggccg ccgaagaaca ttgcttctcg ttcttccttc
     6181 ggtacattca acagattctg caataaacgg aaagcgtcaa aaaccgaaag ggagcataag
     6241 cgagcgtctt catccagcag tggactgaca ggggggaagc gcagcacacg gcagtttggt
     6301 gattgttcat tttccacacc cgcaggcagg gcgttatcca gtagtgtcag cagggcttcg
     6361 ccgttgccgg aaagtgcctg gattgtgaca gtgtcaccta aagctgtaat gcgcagcgca
     6421 ctgtctacca gcagcaggct ttttaaatca tctttgctgt cgatatctgc ggattccagc
     6481 agcagcgttg ccggacgatc cccacacaac tggtgaaaaa gcgcggtggg attgtcgcga
     6541 taagcgcctt cgcaggttag cagttcgaga gtcggttttt gtgtttgcat tgttattctc
     6601 taattttgtt caaaaaaaag cccgctcatt aggcgggctg ggtatctgat tgctttacgc
     6661 atggtgaata cactgcccgt ttcaggaagt gcgccaccaa cctttcagta cgaaaattgc
     6721 tttcattgtc gatacccttt ttacgtgaac ttgcgtacta gttaactggt tcgatgatta
     6781 attgtcaaca gctcatttca gaatatttgc cagaaccgtt atgatgtcgg cgcaaaaaac
     6841 attatccaga acgggagtgc gccttgagcg acacgaatta tgcagtgatt tacgacctgc
     6901 acagccatac cacagcttcc gatggctgcc tgacgccaga agcattggtg caccgtgcag
     6961 tcgagatgcg cgtcggcacc ctggcgatca ccgaccatga caccacagct gcaatcgcgc
     7021 cagcaagaga ggaaatttca cgttcgggac tggcgttgaa tcttattccc ggcgtggaaa
     7081 tttccacggt ctgggaaaat catgaaattc atattgtcgg gctgaatatt gatattactc
     7141 atccgctaat gtgtgagttc ctcgcgcagc agacagaacg gcgcaatcag cgggcgcagc
     7201 tgattgccga aagacttgaa aaagcgcaaa tacctggggc gctggaaggc gcacaacgac
     7261 tggcgcaggg cggcgcagtg acgcgcggtc attttgcgcg tttccttgtt gagtgcggca
     7321 aagccagttc aatggcggat gtctttaaaa agtatctggc gcgcgggaaa accggatacg
     7381 ttccgccaca gtggtgtaca atagaacaag ctattgatgt cattcatcat tctggcggta
     7441 aggcggtgct agctcatcct gggcggtaca atcttagtgc taaatggctg aaaagattgg
     7501 ttgcgcattt tgccgaacac cacggtgacg cgatggaagt cgcgcagtgc cagcaatcgc
     7561 ccaatgaacg tacccagctg gcggcccttg cgcgtcagca tcatttatgg gcatcacaag
     7621 gatctgattt tcatcagcca tgcccgtgga tcgaactggg tcgtaaactc tggctgcccg
     7681 caggcgttga aggcgtctgg cagctatggg aacagccgca gaacaccaca gagagggaat
     7741 tatgagccag tttttttata ttcatcctga taacccacag caacgtctga tcaaccaggc
     7801 ggtggagatc gtgcgtaaag gcggggtgat tgtttatcca actgattccg gctatgcgct
     7861 cggctgtaaa attgaagata aaaacgcgat ggagcgtatt tgtcgtattc gccagctgcc
     7921 ggacggtcac aactttaccc tgatgtgtcg cgatctttct gaactctcga cctattcatt
     7981 tgttgataac gttgcgtttc gtttaatgaa aaacaacacg ccgggcaact ataccttcat
     8041 cttgaagggg acgaaggaag tgccacgccg tctgttgcag gaaaaacgca aaaccatcgg
     8101 tatgcgtgtg ccttctaacc cgattgcgca ggcgttgctt gaagcactgg gcgaaccgat
     8161 gctttccact tcgctaatgc tgccaggcag cgaatttacc gaatccgatc ccgaagaaat
     8221 taaagaccgt ctggaaaaac aggtagattt gattattcac ggcggctatc tcgggcagaa
     8281 accgacaacg gttattgatc ttaccgatga tacgccggtc gtggtgcgtg aaggcgtagg
     8341 tgatgtgaag cctttcttat aagtgcgtaa ctctgtatac tacgcggcct taaaatggcg
     8401 ctatacggcg ccattgattc tcctgttcga cgcctgtgaa ggcgacacta aaaggaagct
     8461 ctatgagcga aaaattacag aaagtgctgg cccgcgctgg ccacggctcc cgccgtgaaa
     8521 tcgaatctat tattgaagcc ggtcgtgtga gtgttgatgg caaaattgct aaactcggcg
     8581 atcgcgttga agttacccct gggctgaaaa ttcgtatcga tggtcatctg atttcggtac
     8641 gtgagtccgc tgaacaaatt tgtcgcgttc tggcctatta caaaccggaa ggtgagttgt
     8701 gtacccgtaa cgatccggaa gggcgtccga cggtgtttga ccgtctgcca aagctgcgtg
     8761 gcgcacgctg gattgccgta ggtcgtctgg acgttaacac ctgtggtctg ctgctgttca
     8821 ccaccgatgg tgaactggca aaccgtttaa tgcatccaag ccgtgaagtt gaacgtgaat
     8881 acgccgtgcg tgtatttggt caggttgacg acgcgaaact gcgtgatttg agtcgtggcg
     8941 tgcaactgga agatggtccg gcagctttta aaaccatcaa gttcagcggc ggcgaaggga
     9001 tcaaccagtg gtacaacgtg actctgaccg aaggccgtaa ccgtgaagtt cgtcgtctgt
     9061 gggaagcggt tggtgtgcag gtgagccgcc tgatccgtgt tcgttacggt gatatcccac
     9121 tgccaaaagg tctgccacgc ggtggctgga cggagctgga cctcgcccag actaactatc
     9181 tgcgcgaact ggtggagcta ccgccagaaa ccagctctaa agtcgctgta gaaaaagacc
     9241 gtcgtcgcat gaaggcgaat cagattcgtc gtgcggtgaa acgtcacagt caggtgagcg
     9301 gcggtcgtcg ttctggcgga cgtaataacg gttaatcaaa gtatgccgga cgtcatatcc
     9361 ggcattttta cagattagta atcaatccct atctgcgctt ttacaccggc atcaaacgca
     9421 tgtttgacgg ggcgtaattc acttaccgta tctgccagtt caagaatatc ccgatgacaa
     9481 ccacgacccg tgataatcac cgtctgttga tgtggacgtt catttaacgc ctgcaccact
     9541 tcttccagtg gcaaatagtc atacgccacc atatacgtca gttcatcaag caaaaccata
     9601 tccagtgagg aatcagcaag catccgcttt gcatgttgcc agacttcgcg gcaggcggcg
     9661 gtatcagact cgcggttttg tgtatcccag gtaaagcccg ttgccatcac ctgaaactca
     9721 acgccatgtg gctccagcag attgcgttcg ccattaggcc aggtgccttt aataaactgc
     9781 acgacgccta ctttttttcc gtgaccaact gcgcgtgttg ccgtaccaaa tgccgcggtg
     9841 gttttgcctt ttccattgcc ggtaaagacg atgataatac cgcgttcatc ctgggcctgg
     9901 gccacacgag catctacttt ttctttcact cgctgctgac gctgttggta gcgttcatca
     9961 ctcattggga aattcctggt ttacggcccg gttgggcgtc aaaggtcatg ccggttttac
    10021 ggcggctgtc atcgcccatc agccagaggt agagcggcat gatatcagcg ggtgttttaa
    10081 gtttctgtgg atcttcggtc gggaaggcgc tggcacgcat tgcggtgcgc gtaccgcctg
    10141 ggttaatgca gttgacacgc aggcgctgct gatattcatc ggccagtacc tgcatcatcc
    10201 cttcggtggc aaatttcgac gctgcatatg caccccagtt agctcgtccc tgacgtccaa
    10261 cgcttgatga agtaaagacc agtgaaccgg cgtccgattt gagtaataaa ggaagcagcg
    10321 cctgggtgag caaaaaggtg gcattaacgt tgacctgcat gacgtcctgc cagacctgcg
    10381 gattttgttc gctcattggg caaacatcgc cgagcaatcc ggcattatgc aaaacaccat
    10441 ccagacgcgg ataattaacg gtaatgcgct gtgccagttg ttggcaatct tcggacgtgc
    10501 aggtcagcaa atcgagaata aaccactgtg gctgacgtcc agtttcttcg tttatgtggc
    10561 tggctacctg acgtaatttt tcttcattac ggcccaacag aatcactgtc gcaccatagc
    10621 gtgcatacgt catcgcggct tcacggccaa tcccatcgct ggctcccgtc accaggataa
    10681 tgcgatcatt gagtaaatct tgttttggct ggtaatgcat ggctactcct caacgacgtt
    10741 gtctgtcgcg ctgccagtgt actttatgac tttggggctt tatgcctgaa acagaagggc
    10801 atttcaatca gcctggggcg ataacaccgc aaaatggata ctttgtcata ctttcgctgc
    10861 aataacatct ctgcgagacg gcttaacatg cctgttgtaa actgtgagcc aaagcgttgt
    10921 ttaaccaagg tggggactcg tggaattgtt gtctgaatat ggtttgtttt tggcgaaaat
    10981 cgttaccgtt gtgctagcga ttgcggcgat tgccgccatt attgtcaatg ttgctcaacg
    11041 taataaacgc cagcgtggcg agttacgggt caacaatctc agcgaacagt ataaggagat
    11101 gaaagaagaa ctggccgcgg cgctgatgga ctcacatcag caaaaacagt ggcacaaagc
    11161 gcagaagaaa aagcacaagc aagaagcgaa agcagcaaaa gcgaaagcca aactgggcga
    11221 ggtggcaact gacagtaaac ctcgcgtctg ggtgctggat tttaaaggca gcatggacgc
    11281 ccatgaagtg aactcgctac gtgaagagat aacggctgta ctcgcagcat tcaaaccgca
    11341 ggatcaggtt gtgctacgtc tggaaagccc tggtggcatg gtgcatggtt acgggttggc
    11401 ggcttcgcag ctgcagcgtc tgcgtgataa aaacattcct ttaactgtta cggtagacaa
    11461 agtcgctgcc agcggcggtt acatgatggc ctgtgtggcg gacaaaattg tttccgcacc
    11521 gtttgctatt gtgggttcca ttggggtggt ggcgcaaatg cccaacttta accgcttcct
    11581 gaaaagcaaa gatattgata tcgaactgca cactgccggg cagtataagc gtactctgac
    11641 gttgctgggt gaaaataccg aagaagggcg ggagaaattc cgcgaagagt tgaacgaaac
    11701 gcatcagttg tttaaagatt ttgtgaagcg tatgcgtccg tctctggata ttgaacaggt
    11761 ggcaacgggt gaacactggt acggacaaca ggcggtagag aaaggcctgg ttgatgaaat
    11821 caataccagt gatgaagtta ttcttagcct gatggaaggc cgtgaagtgg ttaatgtacg
    11881 ctatatgcag cgtaaacgac tcattgaccg attcaccggc agcgcggcag agagcgccga
    11941 tcgattgttg ctacgctggt ggcagcgggg tcaaaagcca ttgatgtaaa agacaaacgc
    12001 gaggctaaga cctcgcgttt tgctttaatc aaccagatga tatttttctg aaagcacatg
    12061 ggccaggtgt ttgaacatat taaacaccgc ggtgcttttg gctgttggca atccttgttc
    12121 atctaaaaag tagtcgccgg taaataccag cacgccatta cgctgcgtga cgccggtggc
    12181 ttcaatccct gcaagcgtat cctcatgctc acgaatgatt ttgttggcct ctttcaacag
    12241 cgtttcgcgg tcgatgggtt gtgtctcttt gttcattatt tactccttaa acaaggacat
    12301 tagtctacgc caggcatggc ttgcagacaa atataccacg ctggtggcaa gagcgcctta
    12361 ctggcaactt tggattttgc atgctaataa agttgcgtat cggattttat caggtacagt
    12421 gtgacgcttt cgtcaatctg gcaatagatt tgcttgacat tcgaccaaaa ttccgtcgtg
    12481 ctatagcgcc tgtaggccaa gacctgttaa ctcagtcacc tgaattttcg tgaacagagt
    12541 cacgacaagg ggttgatatc cgcagagaac gagtccatat cggtaactcg ttgccagtgg
    12601 aaggtttatc aacgtgcgac gcattcctgg aagaatcaaa ttaggtaagg tgaatatggg
    12661 taaagctctt gtcatcgttg agtccccggc aaaagccaaa acgatcaaca agtatctggg
    12721 tagtgactac gtggtgaaat ccagcgtcgg tcacatccgc gatttgccga ccagtggctc
    12781 agctgccaaa aagagtgccg actctacctc caccaagacg gctaaaaagc ctaaaaagga
    12841 tgaacgtggc gctctcgtca accgtatggg ggttgacccg tggcacaatt gggaggcgca
    12901 ctatgaagtg ttgcctggta aagagaaggt cgtctctgaa ctgaaacaac tggctgaaaa
    12961 agccgaccac atctatctcg caaccgacct tgaccgcgaa ggggaagcca ttgcatggca
    13021 cctgcgggaa gtgattgggg gtgatgatgc gcgctatagc cgagtggtgt ttaacgaaat
    13081 tactaaaaac gcgatccgcc aggcatttaa taaaccgggt gagctgaata ttgatcgtgt
    13141 taatgcccag caggcgcgtc gctttatgga ccgcgtggtg gggtatatgg tttcgccgct
    13201 gctatggaaa aagatcgctc gtggtctgtc tgccggtcgt gtgcagtcgg tggcggttcg
    13261 cctggtggtc gagcgtgagc gtgaaattaa agcgttcgtg ccggaagagt tctgggaagt
    13321 cgatgccagc acgaccacgc catctggtga agcgttggcg ttacaggtga ctcatcagaa
    13381 cgacaaaccg ttccgtccgg tcaacaaaga acaaactcag gctgcggtaa gtctgctgga
    13441 aaaagcgcgc tacagcgtgc tggaacgtga agacaaaccg acaaccagta aacctggcgc
    13501 tccttttatt acctctacgc tgcaacaagc tgccagcacc cgtcttggat ttggcgtgaa
    13561 aaaaaccatg atgatggcgc agcgtttgta tgaagcaggc tatatcactt acatgcgtac
    13621 cgactccact aacctgagtc aggacgcggt aaatatggtt cgcggttata tcagcgataa
    13681 ttttggtaag aaatatctgc cggaaagtcc gaatcagtac gccagcaaag aaaactcaca
    13741 ggaagcgcac gaagcgattc gtccttctga cgtcaatgtg atggcggaat cgctgaagga
    13801 tatggaagca gatgcgcaga aactgtacca gttaatctgg cgtcagttcg tagcctgcca
    13861 gatgacccca gcgaaatatg actccacgac gctgaccgtt ggtgcgggcg atttccgcct
    13921 gaaagcacgc ggtcgtattt tgcgttttga tggctggaca aaagtgatgc ctgcgttgcg
    13981 taaaggcgat gaagatcgca tcttaccagc agttaataaa ggcgatgctc tgacgctcgt
    14041 tgaacttaca ccagcccagc actttaccaa gccgccagcc cgtttcagtg aagcatcgct
    14101 ggttaaagag ctggaaaaac gcggtatcgg tcgtccgtct acctatgcgt cgatcatttc
    14161 gaccattcaa gatcgtggct acgtgcgagt agaaaatcgt cgtttctatg cggaaaaaat
    14221 gggcgaaatc gtcaccgatc gccttgaaga aaatttccgc gagttaatga actacgactt
    14281 caccgcacag atggaaaaca gcctcgacca ggtggcaaat cacgaagcag agtggaaagc
    14341 tgtactggat cacttcttct cggatttcac ccagcagtta gataaagctg aaaaagatcc
    14401 ggaagagggt ggtatgcgcc cgaaccagat ggttctgacc agcattgact gcccgacttg
    14461 tggtcgcaaa atggggattc gcacagcgag caccggggta ttccttggct gttctggtta
    14521 tgcgctgccg ccgaaagagc gttgcaaaac caccattaac ctggtgccgg aaaacgaagt
    14581 gctgaacgta ctggaaggcg aagacgctga aaccaacgcg ctgcgcgcaa aacgtcgttg
    14641 cccgaaatgc ggcacggcga tggacagcta tctcatcgat ccgaaacgta agttgcatgt
    14701 ctgtggtaat aacccaacct gcgacggtta cgagatcgaa gagggcgaat tccgcattaa
    14761 aggttatgac ggcccgatcg ttgagtgtga aaaatgtggc tctgaaatgc acctgaaaat
    14821 ggggcgtttc ggtaaataca tggcctgcac caacgaagag tgtaaaaaca cacgtaagat
    14881 tttacgtaac ggcgaagtgg caccaccgaa agaagatccg gtgccattac ctgagctgcc
    14941 gtgcgaaaaa tcagatgctt atttcgtgct gcgtgacggt gctgccggtg tgttcctggc
    15001 tgccaacact ttcccgaaat cgcgtgaaac gcgtgcgcca ctggtggaag agctttatcg
    15061 cttccgcgac cgtctgccgg aaaaactgcg ttatctggcc gatgcgccac agcaggatcc
    15121 ggaaggtaat aagaccatgg ttcgctttag ccgtaaaacc aaacagcaat atgtctcttc
    15181 ggaaaaagac ggaaaggcga ctggctggtc agcattttat gttgatggca aatgggttga
    15241 aggaaaaaaa taacctttaa ttctgtcagg tttttataaa caaagggtcg cgaaagcggc
    15301 ccttttttat tgcatattat tttttcttca cacctataca ctaaggctat aaatgatata
    15361 gtggttatag ttagcacctt ttttattatt aaatcgtatt agtcacccgc caggtgtgac
    15421 gaaaaaacga tgttctgatg gcgtctaagt ggatggttta acatgaaatt acaacaactt
    15481 cgctatattg ttgaggtggt caatcataac ctgaatgtct catcaacagc ggaaggactt
    15541 tacacatcac aacccgggat cagtaaacaa gtcagaatgc tggaagacga gctaggcatt
    15601 caaatttttt cccgaagcgg caagcacctg acgcaggtaa cgccagcagg acaagaaata
    15661 attcgtatcg ctcgcgaagt cctgtcgaaa gtcgatgcca taaaatcggt tgccggtgag
    15721 cacacctggc cggataaagg ctcgctgtat atcgccacca cgcataccca ggcacgctac
    15781 gcgttaccaa acgttatcaa aggctttatt gagcgttatc cccgcgtttc tttgcatatg
    15841 caccagggct cgccgacaca aattgctgat gccgtctcta aaggcaatgc tgatttcgct
    15901 atcgccacag aagcgctgca tctgtatgaa gatttagtga tgttaccgtg ctaccactgg
    15961 aatcgggcta ttgtagtcac tccggatcac ccgctggcag gcaaaaaagc cattaccatt
    16021 gaagaactgg cgcaatatcc gttggtgaca tataccttcg gctttaccgg acgttcagaa
    16081 ctggatactg cctttaaccg cgcagggtta acgccgcgta tcgtcttcac ggcaacggat
    16141 gctgacgtca ttaaaactta cgtccggtta gggctggggg taggggtcat tgccagcatg
    16201 gcggtggatc cggtcgccga tcccgacctt gtgcgtgttg atgctcacga tatcttcagc
    16261 cacagtacaa ccaaaattgg ttttcgccgt agtactttct tgcgcagtta tatgtatgat
    16321 ttcattcagc gttttgcacc gcatttaacg cgtgatgtcg ttgatgcggc tgtcgcattg
    16381 cgctctaatg aagaaattga ggtcatgttt aaagatataa aactgccgga aaaataattt
    16441 cttgcgttat tttcggcacc ttttatgtag cgaaggtgcc ggaatatatt ctcttttgtt
    16501 acttatttct ataccgtgca caatattttt atccctcata tttatagggt aaattacctg
    16561 taaagtagtg tctaattacc cgcctcgcaa actccagttt tcaaatatct atttcaggtc
    16621 aaaagattga attattcact ggagacgatt cgtaaattcg ctggattttt gactaaagtt
    16681 tctttaggat ttgtctcatc gtatgattaa ttacaccaat cgttcaacgt cttgctgata
    16741 agtgatggcg attatatgag gttagcaatg ccttctggaa atcaggaacc ccgccgcgat
    16801 cctgaattaa aacgtaaagc ctggctggcg gtttttcttg gttctgcact tttctgggtg
    16861 gttgtcgcac tgctgatttg gaaagtgtgg ggataactat ggtcggtcag gagcaactgg
    16921 agtcgtcacc attatgccag catagtgaca atgaaacaga aacgaaacgg gaatgttccg
    16981 tcgttattcc agacgactgg caactaacat cgcagcagca agcctttata gaactgtttg
    17041 ctgaagatga tcagcc
//
LOCUS       DAPGWD010000090        16924 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPGWD010000090 DAPGWD010000000
VERSION     DAPGWD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.091"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            10..1299
                     /locus_tag="RHX88_002864"
     CDS             10..1299
                     /locus_tag="RHX88_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HDV2231533.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVGEEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1296..2762
                     /gene="hpaE"
                     /locus_tag="RHX88_002865"
     CDS             1296..2762
                     /gene="hpaE"
                     /locus_tag="RHX88_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HDV2231534.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2764..3615
                     /gene="hpaD"
                     /locus_tag="RHX88_002866"
     CDS             2764..3615
                     /gene="hpaD"
                     /locus_tag="RHX88_002866"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HDV2231535.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGIYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3625..4005
                     /locus_tag="RHX88_002867"
     CDS             3625..4005
                     /locus_tag="RHX88_002867"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HDV2231536.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4073..4876
                     /gene="hpaH"
                     /locus_tag="RHX88_002868"
     CDS             4073..4876
                     /gene="hpaH"
                     /locus_tag="RHX88_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HDV2231537.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4887..5675
                     /gene="hpaI"
                     /locus_tag="RHX88_002869"
     CDS             4887..5675
                     /gene="hpaI"
                     /locus_tag="RHX88_002869"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HDV2231538.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5798..7174
                     /gene="hpaX"
                     /locus_tag="RHX88_002870"
     CDS             5798..7174
                     /gene="hpaX"
                     /locus_tag="RHX88_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HDV2231539.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSLVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7184..8074
                     /gene="hpaA"
                     /locus_tag="RHX88_002871"
     CDS             7184..8074
                     /gene="hpaA"
                     /locus_tag="RHX88_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HDV2231540.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8325..9887
                     /gene="hpaB"
                     /locus_tag="RHX88_002872"
     CDS             8325..9887
                     /gene="hpaB"
                     /locus_tag="RHX88_002872"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HDV2231541.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            9905..10417
                     /locus_tag="RHX88_002873"
     CDS             9905..10417
                     /locus_tag="RHX88_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HDV2231542.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10810..12960
                     /gene="btsT"
                     /locus_tag="RHX88_002874"
     CDS             10810..12960
                     /gene="btsT"
                     /locus_tag="RHX88_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDV2231543.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13010..13213
                     /locus_tag="RHX88_002875"
     CDS             13010..13213
                     /locus_tag="RHX88_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV2231544.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13224..14180
                     /gene="yjiA"
                     /locus_tag="RHX88_002876"
     CDS             13224..14180
                     /gene="yjiA"
                     /locus_tag="RHX88_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDV2231545.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14258..16798)
                     /locus_tag="RHX88_002877"
     CDS             complement(14258..16798)
                     /locus_tag="RHX88_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HDV2231546.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESSYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYIHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPLSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVDTLTRWDGINLLNDD
                     GKTWQQPGSVILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVFHVQR"
BASE COUNT         3948 a         4624 c         4506 g         3846 t
ORIGIN      
        1 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
       61 gcatggcagg aagcgttcca gcaatccccc tacaaagccc cgcctaaaac tgcggtctgg
      121 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
      181 gaaaaggtac tgagcggtgc gactgttgcg ctgattgtgg gaaaaacggc gacgaaagta
      241 ggtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
      301 ccggaagaga gcttttaccg cccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
      361 attggcgaaa ccgtggctct cagcaatgtc gataatctga ccatctatac cgagatcaac
      421 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc gcagttgctg
      481 agcgccctga gcgaatttgc cacgctgaat ccaggcgacg ccattctgct cggcacgcca
      541 caggcgcgcg tggaaataca gccaggcgat cgcgttcgtg ttctcgcaga aggtttcccg
      601 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
      661 cagccacacc cgcacggcac gctgtttgcc ctcggcctga actacgccga ccacgccagc
      721 gaactggaat ttaagccacc ggaagaaccg ctggtgttcc tgaaagcgcc aaataccctc
      781 actggcgata accagacctc cgtgcgtcca aacaatattg aatacatgca ctatgaagcg
      841 gagctggtgg tagttattgg caaacaggcg cgtaacgtca gcgaagccga tgccatggat
      901 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
      961 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
     1021 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
     1081 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
     1141 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
     1201 ggcttatctg acgtggtgcc tggcgatgaa gtggtggtgg aagtagaagg cgtgggccgc
     1261 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
     1321 acggtaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
     1381 tgctggcgga tgtggcctcc ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
     1441 aagaggcgtt cccgaaatgg gccaatctgc cgatgaaaga gcgcgcgcgc ctgatgcgcc
     1501 gtctgggcga tctgatcgac cagaacgtgc cggagatcgc cgcgatggaa accgcggaca
     1561 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
     1621 aatttttcgc ggaagtctgc cagcagatga acggcaagac ttatccggtc gacgacaaga
     1681 tgctcaacta cacgctggtg cagccggtag gcgtttgtgc actggtgtca ccgtggaacg
     1741 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
     1801 tactgaaaat gtcggaactc tccccgctga ccgccgaccg cctgggtgag ctggcgctgg
     1861 aagctggtat tccggcgggc gtgctgaacg tggtacaggg ctacggcgca accgcaggcg
     1921 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt acggcgaccg
     1981 ggcgcaacat catgaaaaac gccgggctga aaaaatactc catggaactg ggcggtaaat
     2041 cgccggtgct gatttttgaa gatgctgata ttgaacgcgc gctggacgcc gccctgttca
     2101 ccatcttctc gatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaaa
     2161 gcatctaccc ggaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
     2221 atccgaacga tccgaatacc caggttggcg cgcttatcag ccagcagcac tgggaaaaag
     2281 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgctggcg ggcggcccgg
     2341 ataaaccgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaaccgtgc
     2401 tggcagacgt tgataaccgt atgcgcgtcg cccaggaaga gattttcggg ccggtcgcct
     2461 gcctgctgcc gtttaaagac gaagctgaag gcttacgcct ggcaaacgac gtggagtacg
     2521 gcctcgcgtc gtacatctgg actcaggatg tcagcaaagt gctgcgtctg gcgcgtggca
     2581 ttgaagctgg catggtgttc gtcaacaccc agaacgtgcg tgatttgcgc cagccgtttg
     2641 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
     2701 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
     2761 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
     2821 gaactgccag ggaaaaacca cggttgccgc cagggcgcga tcgacgggca taaagagatc
     2881 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac ccactggctg
     2941 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcatcta caccagcaac
     3001 gagctgccgc attttatccg cgatatgacc tacaactacg agggcaaccc ggagttgggg
     3061 cagcttattg ccgatgaagc cttaaagctc ggcgtgcggg caaaagcgca caacattccc
     3121 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
     3181 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgctga cagccgcaag
     3241 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
     3301 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cagaagaagg gatgaacagc
     3361 tacacccgcg agttcgaccg ccagatggac gagcgcgtgg tgaagttgtg gcgcgaaggc
     3421 cagttcaaag agttctgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
     3481 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
     3541 gtggagttta tcaccgagct attcccaagc tctggcaccg gtcaggttaa cgctgttttc
     3601 ccgcttcccg cgtaaggaac caccatgccg cactttatcg tggaatgtac tgaaaatatt
     3661 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
     3721 gggatttttc cgcttggggg aatacgtagc cgggcacact ggatcgatac ctggcaaatg
     3781 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcgggcgc
     3841 agtctggaaa gccgtcagga agtaggcgaa atgctgtttg atctgattaa aacgcacttc
     3901 gcctcactga tggagagccg ttacctggcg ctttcatttg agattgcaga gctgcacccg
     3961 acgctgaatt ttaaacaaaa caacgtgcac gcgttgttta aataaatttg caccagcttc
     4021 caggccggat gataaggcgc agccgcatcc ggcaatgcca caggatatcg ctatgttcga
     4081 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
     4141 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgctt acgcggtgca
     4201 gcgtgaatgg gttcgactga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
     4261 cctgacctcg aaagcgatgc aggccagctc acaaatcagc gagccggatt acggtgccct
     4321 gctggatgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
     4381 gcgcattgaa gtggagctgg ctttcgtgct ggcaaaaccg ctgcgtgggc caaactgcac
     4441 gctgttcgac gtctacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
     4501 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtgttcg acaccatttc
     4561 cgataacgcc gccaacgccg gggtgatcct cggcggtcgt ccgatcaaac cagacgaact
     4621 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
     4681 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
     4741 accgtatgac gtacaactgg aagccgggca aatcattctc ggcggttcgt tcacccgccc
     4801 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccattag
     4861 ctgccgcttt gtttaaggag agaacgatgg aaaacagttt taaagcggcg ctgaaagcag
     4921 gccgtccgca gattggatta tggctggggc tgagcagcag ctacagcgcg gagttactgg
     4981 ccggagcagg attcgactgg ttgttgatcg acggtgagca cgcgccgaat aacgtgcaaa
     5041 ccgtgctcac ccagctacag gcgattgcgc cctaccccag ccagccggta gtacgtccgt
     5101 cgtggaacga tccggtgcaa atcaaacaac tgctggacgt cggcacacaa accttactgg
     5161 tgccgatggt tcaaaacgct gacgaagccc gtgaagcggt acgcgccacc cgttatcccc
     5221 ccgccggtat tcgcggtgtg ggcagtgcgc tggcccgcgc ctcgcgctgg aatcgtattc
     5281 ctgattacct gcaaaaagcc aacgatcaaa tgtgcgtgct ggtgcagatc gaaacgcgtg
     5341 aggcaatgaa gaacttaccg cagattctgg acgtggaagg agtcgacggc gtgtttatcg
     5401 gcccggcgga tctgagcgcc gatatgggtt atgccggtaa tccacagcac ccggaagtac
     5461 aggccgccat tgagcaggcg atcgtgcaga tccgcgaagc gggcaaagca ccggggatcc
     5521 tgatcgccaa tgagcaactg gcaaaacgct atctggaact gggcgcgctg tttgtcgccg
     5581 tcggcgttga caccaccctg ctcgcccgcg ccgctgaagc gctggcagca cggtttggtg
     5641 cgcaggctac cgccgtgaag cccggcgtgt attaatgccg gatgcgctgc gcttatccgg
     5701 cctacactcg caccaaatcg taggccggat aaggcgttac gccgcatcca gcaaaaacct
     5761 tgtcgtaccc tacaaaaatc ccattagagg aagaaaaatg agcgacacct cacctgccat
     5821 accggagagt atcgatccgg cgaatcagca taaagcgctg actgcaggac aacaggcggt
     5881 tattaagaag ctatttcgcc gcctgatcgt ctttctgttc gtgctgttta tcttctcgtt
     5941 ccttgatcgc atcaacatcg gctttgccgg actcacgatg ggacgcgacc tcggtctgag
     6001 cgccaccatg tttggcctcg ctaccaccct gttctacgcc gcttatgtca tcttcggcat
     6061 tcccagcaac attatgctga gtattgtcgg tgcgcggcgc tggatcgcca ccatcatggt
     6121 gctttggggc atcgcctcta ctgccaccat gtttgccact ggccccacca gcttgtacgt
     6181 actgcgtata ctggttggca ttaccgaagc cggctttctg cctggcattc tgctgtattt
     6241 aaccttctgg tttccagcct atttccgcgc ccgtgccaac gccttgttta tggttgcaat
     6301 gccggtaacg acagcgttgg gatcgctcgt ttccggctac attttgtcgc tggatggcgt
     6361 aatggcatta aaaggctggc agtggctgtt tttgctggaa ggcttcccgt cggtattact
     6421 cggcgtcatg gtgtggttct ggcttgatga ctcaccggac aaagctaagt ggctgacgaa
     6481 agaagacaaa aaatgcctgc aagagatgat ggataacgat cgtctgacgc tggttcagcc
     6541 agagggagcc atcagccacc acgccatgca acaacgcagc atgtggcggg agatcttcac
     6601 tccggtggtg atgatgtata ccctggcgta tttctgcctg accaacacac ttagtgcgat
     6661 cagcatctgg acaccgcaga tcctgcaaag ctttaatcag ggcagcagta atatcaccat
     6721 cggcctgctg gccgccgtac cgcagatttg taccattctc gggatggtct actggagccg
     6781 tcactcagat cgccgccagg aacgaaggca tcacaccgcc cttccttatt tgttcgctgc
     6841 cgcaggttgg ttgctggctt cggcaactga tcacaacatg atccagatgc tggggatcat
     6901 tatggcttcg accggatcat tcagcgcaat ggcgattttc tggacaacac cggatcagtc
     6961 catcagcctg cgggcacgag cgatcggtat tgcggtgatc aacgccactg gcaacattgg
     7021 ttcagcgtta agtccgttta tgatcggctg gttgaaagat ttgaccggca gctttaacag
     7081 tggattgtgg tttgttgccg cgctgctggt gattggtgcg gggattatct gggcgattcc
     7141 aatgcagtcc tcccgtccgc gagcgacccc gtaaggaacg acgatgtgtg accgtcagat
     7201 tgccaatatt gatatcagca aagagtacga tgaaagcctg ggcacggacg atgtgcatta
     7261 tcagtcgttc gcccgcatgg cggccttttt tggccgccat atgctgccac atcgccacga
     7321 acagtacttt cagatgcatt tcctcaatag cggacagatt gagctacaac ttgacgatca
     7381 tcgctactcg gtggaagcgc ccctgtttgt cctgacgccg ccgtcagtac ctcatgcgtt
     7441 tattacggag tctgacgccg acggtcatgt gttgacggta cgggaagatc tgatctggcc
     7501 cctgctggaa gttctttatc cgggcactag ggaaaccttc ggcctgccgg ggatttgcct
     7561 gtcactggca gataaacccg acgaactggc ggcgctggaa cactattggc aactgataga
     7621 gcgggaatcg gtagaacaac tgcctggacg ggaacacacc ctgacgttac tggcacaggc
     7681 agtattcacc ctactgctgc gtaatgcaaa actcgacgac catgccgcca gcggaatgcg
     7741 cggagaatta aaactgttcc agcgttttaa tatgctgatt gaaagccatt ttcatcagca
     7801 ctggacagta ccggattacg ctaacgaact gcatatcacc gaatcacgcc tcacggacat
     7861 ctgccgccgc tttgccaacc gtccaccaaa acggttgatt ttcgacaggc agctgcgaga
     7921 agccaaacgg ctgctgctgt tttctgataa cgcagtgaac aatattgcct ggcaactcgg
     7981 ttttaaagat ccggcttatt ttgcccgctt ttttaatcgc ttagtcggtt gctcgcccag
     8041 tgcttatcgc gccaaaaaag tacctgtgac gtgaaaatcc cttagcctta acgggaaacc
     8101 aggcaccacc tgctattccc ctttctccgc ctggagcaag gggatttagt aacgtcaaaa
     8161 atcgctaaaa gcgacctcga tcacaaaacc gtacccgacc tgaaaagtac cagccattgt
     8221 cccaaaagtc tcttcccgta atcgcccctg caagcttcaa ttgaataaca aaaacaacac
     8281 acaaaattaa caatcgtatt ccgattaata ctgtagaggt cgacatgaaa ccagaagatt
     8341 tccgcgccag tacccaacgt ccgttcaccg gggaagagta tctgaaaagc ctgcaggatg
     8401 gtcgcgagat ctatatctat ggcgagcgag tgaaagacgt caccactcat ccggcatttc
     8461 gtaatgcggc agcgtctgtt gcccagctgt acgacgcact gcacaaaccg gagatgcagg
     8521 actctctgtg ctggaacacc gacaccggca gcggcggcta tacccataaa ttcttccgcg
     8581 tggcgaaaag tgccgacgac ctgcgccagc aacgcgacgc catcgctgag tggtcacgcc
     8641 tgagctatgg ctggatgggc cgtaccccag actacaaagc tgctttcggt tgcgcactgg
     8701 gcgcgaatcc gggcttttac ggtcagttcg agcagaacgc ccgtaactgg tacacccgta
     8761 ttcaggaaac tggcctctac tttaaccacg cgattgttaa cccaccgatc gatcgtcatt
     8821 tgccgaccga taaagtaaaa gacgtttaca tcaagctgga aaaagagact gacgccggga
     8881 ttatcgtcag cggtgcgaaa gtggttgcca ccaactcggc gctgactcac tacaacatga
     8941 ttggcttcgg ctcggcacaa gtaatgggcg aaaacccgga cttcgcactg atgttcgttg
     9001 cgccaatgga tgccgatggc gtgaaattaa tctcccgcgc ctcttatgag atggtcgcgg
     9061 gtgctaccgg ctcgccgtac gactacccgc tctccagccg ctttgatgag aatgatgcga
     9121 ttctggtgat ggataacgtg ctgatcccat gggaaaacgt actgatctac cgcgattttg
     9181 atcgctgccg tcgctggacg atggaaggcg gtttcgcccg tatgtatcct ctgcaagcct
     9241 gtgtgcgcct ggcagtgaaa ctcgacttca ttacggcact gctgaaaaaa tcactcgaat
     9301 gtaccggcac cctggagttc cgtggtgtgc aggccgatct cggtgaagtg gtggcgtggc
     9361 gcaacacctt ctgggcattg agtgactcga tgtgttctga agcgacgccg tgggtcaacg
     9421 gggcttattt accggatcat gccgcactac aaacctatcg cgtactggca ccaatggcct
     9481 acgcgaagat caaaaacatt atcgaacgca acgttaccag tggcctgatc tatctccctt
     9541 ccagtgcccg tgacctgaac aatccgcaga tcgaccagta tctggcgaag tatgtgcgcg
     9601 gttcgaacgg gatggatcac gtccagcgca tcaagatcct caaactgatg tgggatgcca
     9661 ttggcagcga gtttggtggt cgtcacgaac tgtatgaaat caactactcc ggtagccagg
     9721 atgagattcg cctgcagtgt ctgcgccagg cacaaagctc cggcaatatg gacaagatga
     9781 tggcgatggt tgatcgctgc ctgtcggaat acgaccagaa cggctggact gtgccgcacc
     9841 tgcacaacaa cgacgatatc aacatgctgg ataagctgct gaaataacgc agcaggaggt
     9901 taagatgcaa ttagatgaac agcgcctgcg ctttcgtgac gcgatggcca gcctgtcggc
     9961 agcggtaaat attatcacca ccgagggcga cgccggacaa tgcgggatta cggcaacggc
    10021 cgtctgctcg gtcacggata caccaccatc gctgatggtg tgcattaacg ccaacagtgc
    10081 gatgaacccg gtttttcagg gcaacggcaa gttgtgcgtc aacgtcctca accatgagca
    10141 ggaactgatg gcacgccact tcgcgggcat gacgggcatg gcgatggaag agcgttttag
    10201 cctctcatgc tggcaaaaag gtccgctggc gcagccggtg ctaaaaggtt cgctggccag
    10261 tcttgaaggt gagatccgcg atgtgcaggc aattggcaca catctggtgt atctggtgga
    10321 gattaaaaac atcatcctca gtgcagaagg tcacggactt atctacttta aacgccgttt
    10381 ccatccggtg atgctggaaa tggaagctgc gatttaagct ccagcccctt cacgcattcg
    10441 tcgaaggggc atttttattg tgatctcaat cacatatcca cccgatgaat aattcttgcc
    10501 caaccgctca ccgccattcc tgaccgctta catccctaaa ataaccactc agttatttac
    10561 cttactttac gcgcgcgtaa ctctggcaac atcactgcag gatagcggtc aatttacctc
    10621 ctcaaacgca acgcaaacct agaacggctt cggcctatga ttaacaaatt actctatgaa
    10681 ttttggtttg caggaaggca gcaagtgagt gaatccccgg gagcttgcaa cagtaagtga
    10741 caggggtgaa cgaacgcagc tgccgcacct gtaagccaaa agacgacgag taaaatgcca
    10801 ggttttacta tggatacgaa aaaactattc aagcacatac cctgggtgat tctcggaatc
    10861 atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt cagcgccctg
    10921 tggatcgtgg tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta cagtctgtac
    10981 atcgcccaga aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt tattaacaac
    11041 gacggtctga actacgttcc gaccaaccgt tacgtgttgt ttggtcacca cttcgccgct
    11101 atcgccggtg ctggtccgct ggtcggtccg gttctcgccg cacagatggg ctacctgcct
    11161 ggcacgctgt ggctgctggc gggggtagta ctggccggtg cggttcagga ctttatggtg
    11221 ctgtttatct cctctcgccg taacggcgca tctctcggtg agatgataaa agaagagatg
    11281 ggaccagtac cggggactat cgcgctgttt ggctgtttct taatcatgat catcatcctt
    11341 gccgtcctgg cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg tgtcttcacc
    11401 gtttgctcaa ccgtaccgat tgcgctgttt atgggtatct acatgcgctt tatccgtccg
    11461 gggcgtgtgg gtgaagtctc tgtcattggt atcgtgctgc tggttgcctc tatctacttc
    11521 ggtggcgtga ttgctcacga tccgtactgg ggtccggcac tgacctttaa agacaccacc
    11581 attaccttcg cgctgattgg ctatgcgttt gtttccgcac tgctgccggt gtggctgatc
    11641 ctcgcaccgc gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt cggcctggcg
    11701 ttgggtatcg tggtgctgaa cccggaactg aaaatgcctg cgatgaccca gtacattgac
    11761 ggtactggcc cgctgtggaa aggcgctctg ttcccgttcc tgttcatcac catcgcctgt
    11821 ggtgcggtat ctggcttcca cgcgctgatc tcttccggta cgacgccgaa actgctggct
    11881 aacgaaaccg acgcgcgttt cattggctac ggcgcaatgc tgatggagtc cttcgtggcg
    11941 attatggcgc tggttgcagc gtccatcatc gaaccgggtc tttacttcgc gatgaacacc
    12001 ccgcctgcgg gccttggcat caccatgcct aacctgcatg aaatgggtgg cgagaacgcg
    12061 ccgatcatca tggcgcagct gaaagacgtt accgcacacg cggcggcgac cgtcagctcc
    12121 tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat tggtgagcct
    12181 tctgtcctga accgtgcagg tggtgcgccg acactggcgg taggtatcgc gcacgtgttc
    12241 cacaaagtgc tgccgatggc tgacatgggc ttctggtatc acttcggtat tctgttcgaa
    12301 gccctgttca tcctgaccgc gctggatgcg ggtacccgtt ctggccgctt tatgctgcag
    12361 gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg actctctggt tgccggtatc
    12421 atcggtactg cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg cgtggtcgat
    12481 ccgctgggcg gcgttaagag cctgtggccg ctgttcggta tttccaacca gatgctggca
    12541 gccgtagcgc tggtactggg caccgttgtg ctgattaaga tgaagcgcac ccaatacatc
    12601 tgggtaactg ttgttccggc tgtatggctg cttatctgca ccacctgggc gctgggtctg
    12661 aaactgttca gcaccaaccc gcagatggaa ggcttcttct acatggcaag ccagtacaaa
    12721 gagaagattg ctaacggtac tgacctgacg gcgcagcaga ttgccaacat gaaccacatc
    12781 gttgtgaaca actacaccaa cgcgggcctg agtattctgt tcctgattgt ggtgtacagc
    12841 atcatcttct acggtttcaa aacctggctt gcggtgcgta acagcgacaa acgtactgac
    12901 aaagaaacac cgtacgttcc aatcccggaa ggcggcgtga agatctcttc gcaccactaa
    12961 ccgtatttag ccccgcttcg gcggggcttt gttcttatca gagtgaacta tgtttggtaa
    13021 cttaggacag gcaaaaaaat atctcggcca ggcggcgaag atgttgattg gcattccaga
    13081 ctacgacaac tacgtcgagc atatgaagac caaccatccc gacaagccgt acatgagcta
    13141 tgaagaattc ttccgcgaac gccagaacgc gcgctacggc ggcgatggta aaggcggtat
    13201 gcgctgttgt taatggagag agcatgaacc cgattgcagt taccctactc accggttttt
    13261 taggcgcggg caaaaccacc ctgctgcgcc atattcttaa tgaacaacac ggctacaaga
    13321 ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga tgatcaattg attggtgatc
    13381 gcgcaacgca gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc tccaacgagc
    13441 tggaggacgc gctactcgac ctgctggata atctcgacaa gggcaatatt cagttcgatc
    13501 gtctggtcat tgaatgcacc ggcatggccg atcctggccc gattattcaa acctttttct
    13561 cccatgagat tttatgccag cgctacctgc tggacggcgt gattgcgctg gtggatgcgg
    13621 tacatgccga tgagcagatg aaccagttca ccatcgccca gtcccaggtg ggctacgccg
    13681 accgtattct gctgactaaa accgacgtcg caggcgaagc agaaaaactg cgtgaacgcc
    13741 tggcgcgcat caacgcccgt gcaccggtct acaccgtcac ccacggcgac atcgacctgg
    13801 gtctgctgtt caacaccaac ggttttatgc tggaagaaaa cgtcgtcagc accaaaccgc
    13861 gtttccattt tatcgcggac aaacaaaacg atatttcgtc gattgtggtg gaactggatt
    13921 acccggtaga tatcagcgaa gtttcccggg tgatggaaaa cctgctgctg gaatcggcgg
    13981 ataaactgct gcgttacaaa gggatgctgt ggattgacgg cgaacctaac cgcctgctgt
    14041 tccagggcgt ccagcgcctc tacagcgccg actgggacag gccatggggc gatgaaacac
    14101 cgcatagcac gatggtgttt atcgggattc agttgccgga ggatgagatt cgggcggcat
    14161 ttgcgggact caaaaagtaa ccagccctcc aacatcacaa tacctgagac tttatcaccc
    14221 tcatattcct ccagaatatg agggctttcg ggcttaatta tctctgaacg tgaaacactt
    14281 cctgcgactc cttatgcgcc tccacatcct gcttcgttaa ccagagcgac ttacggccaa
    14341 aattttcgta cattttcagc tgatcttcat agtgcttatc aactgttcca tcgggagcaa
    14401 taaacccact ctgaccgggt gcgaccacat cccaggcaag cacaggacga tcgcttgtcg
    14461 ttggtgagaa aacaatcata tcgttttctg ttccacggtt ttgatactcc gcctgatgac
    14521 gcgtttcttc cgctgcggcc tgcggtacac caaagaaatt atttgcccgg aacgttaagg
    14581 ccattgcagg tgttttccag ttactcacat tattgccata gcgtttggaa agagtctccc
    14641 aggtatcttc cagcgcagcc aacacaacct cctgctgtgg tttcccagca aacagatcaa
    14701 ccgcctgtgg gattggtgat ttgtctccct gcaccgcctc atacaaaatt tttgctccaa
    14761 cacttatatt cagcgaacca gttgggccgt cctgggttgt ttcgtagcca ctggcgctgt
    14821 accacttatc aaatggcata ggtacggcag ccactacggt acgcttcaac atactggtca
    14881 gccaaacgtt caggatgaca gagcctggct gctgccaggt tttaccatca tcattaagca
    14941 aattgatgcc atcccaacgt gttaatgtat ccaccaactg acggcgcgga tcgctctgtg
    15001 tcaaaccaga tgtcgctgct tgcagagtag gtaaaaaaag cctcaggtta agatcctgac
    15061 gactggtttg gcgaataaca tcccatgcct gatcagcagt taagcgtggc ttttgctcaa
    15121 gcagtcggtc gatctccgta acgcgatctg ccccccccca caaaaaggca aacagatccg
    15181 aagcgggata atctttttgg ggagaattgt tccagttagc aatatatccc gacagggggt
    15241 tatacacctt agggttcatt tcaaaaggca atagcccttt ccagtcccat tttcccgtac
    15301 caggaacggg taatcgcgga tcatggcctg attgacgatc tggataagca ccagtatgaa
    15361 tataaccaat attgccgttt acatcagcat agtaccagtt gatggtcagt gcttgtttcg
    15421 ctgcctgctg tgtccactcc tgccaatttt tggccttcat ctgatgagtc caggccagca
    15481 aagacgccac ctctttacca tcccatgcgc gggatttagc gtaagccgtt tgtgtcgtct
    15541 ggtcagtttg gagaatgttg ccatgcaccg tacgccagac agtaaaggtc tctgcctgac
    15601 catttttcac cgtaatggtt tcctcacggc ttaacatttt cacccactta ccattatgca
    15661 agtagtagcc tggtttctct gccgacagcc gttcagcaaa aatatcgaca tcatcgccga
    15721 aacctgccgt tgatccccag gaaatcacac cattatgacc aaaaaccagc ccaggatagg
    15781 caaatggtgt attgccagtg acatcataac cagcaccgtg cagaccaata ccataagtat
    15841 acgcaggcgc ataccagcca aactgcggac catttaccat gattgctttc gcatcctggg
    15901 ctttgctttt gccgatcacc cacatattgc tggtcgttgg ataccccgcc agaccattgg
    15961 caccaccctg tgcaaattgt gcagcaatag tttcccggtt cttccctgct gttaacgcca
    16021 gcagtgcgcc atccgcccct tttgctggtc ggtcaagcat tggtgcaggt aaatcgtagc
    16081 gtggcaacag agctgctgtt tgcgagtttt gctgattaaa tttaagtggg tagctactct
    16141 cttgtacggc aatagtggtt ggcgctgatg ggtttaccag ccatttcaac tgattaaata
    16201 ccgccatgcc ttgtgataca ccatatttat cttttaaagc cgttagcagt gccagattat
    16261 caatttcgct agtgctatca gagaagcggt ttgccatggt gcccacaaat atcatcgcga
    16321 catcaaacgg ttcccagcgc ttaggagtaa agccaaatgt attaaactgt tttggtaaga
    16381 gcgtctctgg attggtattt accttatcaa tccaggcatt cattccatca gcgtagcctt
    16441 gcagaatgga catatcctct ggggaaaggg cagcaatttg cgcccggata gcatccggcc
    16501 agtagttacg acggatatct ttatcaaatt tcacaaaatc tttgccaagc acttccgcga
    16561 cagtcccttg agtactgcga cgtgccattt ccatctgaaa aaggcgatct tgtgctacta
    16621 catagccata gccataaaat aggtgccatg tatcattggc ataaatatgc ggcatgccgt
    16681 attcatcgcg aacaatcttt atctcacttg acgactgctc agccagtgca ggtaagctcc
    16741 aataatacat cagggaagca gtaacacagt tcacgatcat acgatttcta tttttcattg
    16801 tatcctctgg caggtgtata attagcgaat tgatactagc aacgaagctt cggttatcta
    16861 tttgcagaga attgtttcat tatgaactgt gagcgcgata actaattttt gatacaaaaa
    16921 tttc
//
LOCUS       DAPGWD010000091        16721 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPGWD010000091 DAPGWD010000000
VERSION     DAPGWD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.092"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(396..1592)
                     /gene="hemY"
                     /locus_tag="RHX88_002878"
     CDS             complement(396..1592)
                     /gene="hemY"
                     /locus_tag="RHX88_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDV2231547.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1595..2794)
                     /gene="hemX"
                     /locus_tag="RHX88_002879"
     CDS             complement(1595..2794)
                     /gene="hemX"
                     /locus_tag="RHX88_002879"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV2231548.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQADTP
                     AAAPQGE"
     gene            complement(2816..3556)
                     /gene="hemD"
                     /locus_tag="RHX88_002880"
     CDS             complement(2816..3556)
                     /gene="hemD"
                     /locus_tag="RHX88_002880"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDV2231549.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3553..4494)
                     /gene="hemC"
                     /locus_tag="RHX88_002881"
     CDS             complement(3553..4494)
                     /gene="hemC"
                     /locus_tag="RHX88_002881"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDV2231550.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4881..7427
                     /gene="cyaA"
                     /locus_tag="RHX88_002882"
     CDS             4881..7427
                     /gene="cyaA"
                     /locus_tag="RHX88_002882"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDV2231551.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7467..7787)
                     /gene="cyaY"
                     /locus_tag="RHX88_002883"
     CDS             complement(7467..7787)
                     /gene="cyaY"
                     /locus_tag="RHX88_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDV2231552.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            8250..8453
                     /locus_tag="RHX88_002884"
     CDS             8250..8453
                     /locus_tag="RHX88_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2231553.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8490..9314
                     /gene="dapF"
                     /locus_tag="RHX88_002885"
     CDS             8490..9314
                     /gene="dapF"
                     /locus_tag="RHX88_002885"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDV2231554.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9311..10018
                     /locus_tag="RHX88_002886"
     CDS             9311..10018
                     /locus_tag="RHX88_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDV2231555.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10015..10911
                     /gene="xerC"
                     /locus_tag="RHX88_002887"
     CDS             10015..10911
                     /gene="xerC"
                     /locus_tag="RHX88_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDV2231556.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10911..11627
                     /gene="yigB"
                     /locus_tag="RHX88_002888"
     CDS             10911..11627
                     /gene="yigB"
                     /locus_tag="RHX88_002888"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDV2231557.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11711..13873
                     /gene="uvrD"
                     /locus_tag="RHX88_002889"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11711..13873
                     /gene="uvrD"
                     /locus_tag="RHX88_002889"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDV2231558.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(14020..14784)
                     /locus_tag="RHX88_002890"
     CDS             complement(14020..14784)
                     /locus_tag="RHX88_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDV2231559.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15154..16104
                     /gene="corA"
                     /locus_tag="RHX88_002891"
     CDS             15154..16104
                     /gene="corA"
                     /locus_tag="RHX88_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDV2231560.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(16158..16637)
                     /locus_tag="RHX88_002892"
     CDS             complement(16158..16637)
                     /locus_tag="RHX88_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDV2231561.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(16634..>16721)
                     /locus_tag="RHX88_002893"
     CDS             complement(16634..>16721)
                     /locus_tag="RHX88_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HDV2231562.1"
                     /translation="FFLQRHKDFAFKPFFTNFNTIERIENYL"
BASE COUNT         3852 a         4321 c         4464 g         4084 t
ORIGIN      
        1 taataagtat gtgtaggatc aagctcaggt taaatattca ctcaggaagt tattactcag
       61 gaagcaaaga ggattacaga attatctcat aacaagtgtt aagggatgtt atttcccgat
      121 tctctgtggc ataataaacg ggtagatgct cattccatct cttatgttcg ccttagtgcc
      181 tcataaactc cggaatgacg cagagccgtt tacggtgctt atcgtccact gacagatgtc
      241 gcttatgcct catcagacac catggacaca acgttgagtg aagcacccac ttgttgtcat
      301 acagacctgt tttaacgcct gctccgtaat aagagcaggc gtttttttat gcatcagaaa
      361 ggtcccggag gtgcttgcct ccgggtgaga aggaactact gtggtgggtt gttctgcaac
      421 gttaacatca aaccgtcgcg acgcatcgct gcagcttctt ccggcttgtg cagtctgtcc
      481 agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac gttgtttcag cgccgcgcgg
      541 aaggcgagcg atgcttcctg ccattctccg tgcttcatca gtgactggcc cagtgtgctc
      601 cacaacagcg ggcgatcgcc gacgtttttg atctgctggc gcagcacttt ttccagctgt
      661 tccggattgt tggttttcag tcgaggaatc ggcagcagta ggcgatcgtc gtactggcgt
      721 ttcaggccat cgatgataat ttgctgggca gtatcatgat cgtcacattc aataagatgt
      781 tccgccattg ccacctgcaa cgctacctga tgacgcgttt tccggctttg gtttttccac
      841 cagttacgca aaccttcact accgttatcg gcacgcgcct gatccatcag gccaatccat
      901 gcctgttgtt ccagcattgc acgatgttct tcatcaccaa catgggcttt cgccattgat
      961 gggatgatat ccagcagaga actccatgca cctgtgcgga tatacgcctg ttccgccaga
     1021 cgtaatactt ccggatggcg tggcgtaact tccagcagct tatccacgcc gtggcgtgca
     1081 gcatggtttt cattacgggc cagttgcaga cgtacacggg tgatttctac cggaatggtg
     1141 tcattgccgg ccagctccgc tgcgcgttcc agatgttggt tggcgcgcgc ttcatcacca
     1201 cgttgttgcg cggcttcggc agccagtaga tagttcacca ccggttgttc agcgtgatcg
     1261 gcatttttcg ccatcagctt ttcaacttgc tgataatcgc cttccgccag tttcagcagc
     1321 gcctgttcgg tctgcttacg tgcacggcga cgcttacgcc cgacaaacca cccacgggtg
     1381 tgcgcgccag tgcggaagat ccgccgcagt agccactcaa tggcaaacag cacgaccatc
     1441 gccagaatca aaataatcgc gaggcccgtg acgctggttt cgatattgta gttgtcggtc
     1501 tggatcagca cataaccctg atgaccggca atcatcgggc caaccacgat ccccgcaatc
     1561 agcaacacaa agagcaataa cacttttagc atggttattc tccttgcggc gcggctgccg
     1621 gagtatcagc ttgcggtgca ggtgcaggtg caggtgcagg tgcaggttta gcttccgttg
     1681 tccccgctgc cggttgtgcc agcaggttac gcacgcgggt ctgcatcagt ttttccagca
     1741 tcgcctggct ttgcagggtt tccggaagat ccatcgagat attttgctgg cttaactggt
     1801 ccacctcgtc gaggaacgct ttggtggtgg catcatcagt atcgtagtaa gcacgtaccc
     1861 aagtggagac gttctccagc gcctggcgat aagtctcttc ctgatgacgc ggtacagctt
     1921 gtgctgcgac cagcaggcga gagcgaatat tttcgcgcag atagatatcc tgatttggcg
     1981 ctaacagcgg tacggcggtg tcatcacgac ggcgaatcgt aatgaagttg tccataaagt
     2041 tctgccagct tttttgcaga ttgatacgcc attcgctgat ggaactggaa agctcttcac
     2101 cgtcggaatc catcggcgaa ccatcgctgt cattatcggc cagacgcagg ttatctacct
     2161 gatttgaaag ctgattaagc ttaaggatga tgccgtcata atccacctgc gatactgcag
     2221 aaaggctggc gatatcatcg gtaattgccc gacgaacggt aatcagactc gggtcattca
     2281 tatccgccag gctggcgtct gcacttttca gcaacgctgc agcggtcgtg acgtcctgat
     2341 cgctccacag cttccgtccg gcgagtttca ccagaaaatc ggcctgagcc agcagccagg
     2401 ttttagcatc gctgccggaa atggtggcga ccttttgttg gacttcatcc aactgttttg
     2461 ccagcgtttc ttgctgacga ttcgcctgct taagctgcgc agcttgttgc ttaataatgc
     2521 cttccagctc ggctttttgg ctctcctggg ctttttgcaa tgccgtcagt tggttagcca
     2581 gggcatcgct ggtggcggtc tgattgacgg cctgttgttt accccagcca tacaaaccga
     2641 cgcccgccgc cagagcaata gcgatagcca ccgcgctgag aatcaatgcg gtattgttct
     2701 tactcttttt ttctgttgcg acaggttgtg acgtggtgtc cacggcctcc ctggtctctt
     2761 caaccacggc ggaggttttt tcttgttccg tcattatggc ttcctgttat gagagttatt
     2821 gtaatgcccg taaaagcgca tcgttgtcag cgttatcggc gaccttaatg tcttgccagc
     2881 ccagttcccg ggcgagtttc gccaaacgct cactgacgac caatagtcga cagtgtagta
     2941 accagtgctc acgataccat tgtgggatca gcgaccagag ttgctgcaac atttcaccgc
     3001 tggtaacaac gaccgtcgtc acctcgcggg attgccagcg catcgcttct tctgcaccat
     3061 cgtaatggat tgcgcatcgt tgataacatt cacaaaaagt gacctcagca ccgcgcgccg
     3121 tcagggtatc cccaattagc tcacgaccgc cattgccacg taatatcagc gcacgtttgc
     3181 ccgcaatatt ttgtaattca ggtaattgta gcaagacttc gctgatttcc cgatcctgcg
     3241 ggtagagaat cttctgtccg cttacggtat gtagcgccag tgcggtggtg cgtccaatgg
     3301 cgaaataagt aggtagtcgg ggccatttac gatcttgctg atgcagctgt gattgggcaa
     3361 aagcaaccgc gtgttgcgag agggcaaaca acagatcgct ctcccccagc gccgccagtt
     3421 gatcagcaag ttgcggtaat tgtcgacccg gagaaaactc aatcagcgga aaatgccagg
     3481 ccacctgccc cagtgtgcgc agacggctca ctaactcttc tccagcggga gacgggcggg
     3541 tgaccaggat actcatgccg gggcgtctcc gttatagact tcagcgagga tctcgcgcgc
     3601 gccgttattc agtagctctt ctgccagcga aatccccatt tgttcggcat tttgcggcgc
     3661 accgcggcgt tcaccgcgaa taatctgcga accgtccggc gcgccgacca gcgcacgcag
     3721 ccagatttca ccatcaataa gctcggcgta gctaccaatt ggcacctgac atccaccttc
     3781 gagacgggta ttcatggcgc gttctgcggt aacgcgcagt gcagtttcgt ggtgattcag
     3841 cgcggcaagc agctcgcgag tgcgcgtatc atcaaggcgg cattcaatac cgactgcacc
     3901 ttgtcctacc gccggaagag aaatctcggg tggtaacgcg gcgcgaatgc gggactccag
     3961 acctaaacgt tttagtccgg ctacggcaag aatgatggca tcgtattcgc cgttatctag
     4021 tttgctcagg cgagtgccga cgttgccgcg cagggagcgg ataatcagat ccgggcggcg
     4081 ttcagccagt tggcactggc gacgtaaact ggacgtcccg acgatactgc ctgccggtaa
     4141 cgcatccaga ctgtcataat tattggacac aaaggcatcg cgaggatctt cacgctcaca
     4201 aatagtgacc aaccccagac cttgcgggaa ttcaaccggc acatctttca ttgaatgtac
     4261 ggcgatatcg gcgcgattct ccaggagcgc gacttccagc tcttttacaa ataagccttt
     4321 tccgcctact ttcgccagcg gcgtatcaag aatcacatcg ccgcgcgtca ccatcggtac
     4381 cagttcaacc accaggcccg gatggctcgc catcaacttg tctttgacat agtgtgcctg
     4441 ccagagtgca agtgggcttt ggcgtgtggc aattcttaaa acattgtcta acatgcttgt
     4501 taccgtcatt atcatccgtg gtccatccta acatccttgc cagagtgatg tcagtgttgt
     4561 ggtgaaacgt agacgcctgc gcaaaccgta aaatgaggtc tggcagtgga tcctgacagg
     4621 cgtttcacgc cgtcgtaata aggaatttac agagaataaa cggtgctaca cttgtatgta
     4681 gcgcatcttt ctttacggtc aatcagcaag gtgttaaatt gatcacgttt tagaccattt
     4741 tttcgtcgtg aaactaaaaa aaccaggcgc gaaaagtggt aacggttacc tttgacatac
     4801 gaaatatccc gaatgccgcg tgttaccgtt gatgttggcg gaatcacagt catgacgggt
     4861 agcaaatcag gcgatacgtc ttgtacctct atattgagac tctgaaacag agactggatg
     4921 ccataaatca attgcgtgtg gatcgcgcgc ttgctgctat ggggcctgca ttccaacagg
     4981 tctacagtct actgccgaca ttgttgcact atcaccatcc gctaatgccg ggttaccttg
     5041 atggtaacgt tcccaaaggc atttgccttt acacgcctga tgaaactcaa cgccactacc
     5101 tgaacgagct tgaactgtat cgtggaatgt cagtacagga cccaccgaaa ggtgagcttc
     5161 caattactgg tgtatacacc atgggcagca cctcgtccgt agggcaaagt tgttcctctg
     5221 acctggatat ctgggtctgt catcaatcct ggctcgatag cgaagagcgc cagttactac
     5281 aacgtaaatg tagtctgctg gaaagctggg ccgcctcgct gggtgtggaa gtcagcttct
     5341 tcctgattga tgaaaaccgc ttccgtcata atgaaagcgg cagcctgggg ggcgaagatt
     5401 gtggctccac ccagcatata ctgctgcttg acgaatttta tcgtaccgcc gtgcgtctcg
     5461 ccggtaagcg tattctgtgg aatatggtgc cgtgcgacga agaagagcat tacgacgact
     5521 atgtgatgac gctttacgcg cagggcgtgc tgacgccaaa tgaatggctg gatctcggtg
     5581 gcttaagctc gctttctgct gaagagtact ttggtgccag cctttggcag ctctacaaga
     5641 gtatcgattc cccatacaaa gcggtactga aaacactgct gctggaagcc tattcctggg
     5701 aatacccgaa cccacgtctg ctggcgaaag atatcaaaca gcgtttgcac gacggcgaga
     5761 ttgtatcgtt tggtctcgat ccatactgca tgatgctgga gcgtgttact gaatacctga
     5821 cggcgattga agattttacc cgtctggatt tagtacgtcg ctgcttctat ttaaaagtgt
     5881 gcgaaaagct cagccgtgaa cgcgcctgcg taggctggcg tcgcgcagtg ttgagccagt
     5941 tagtgagcga gtggggttgg gacgaagctc gtctggcaat gctcgataac cgcgctaact
     6001 ggaagattga tcaggtgcgt gaggcgcaca acgagttgct cgacgcgatg atgcagagct
     6061 accgtaatct gatccgcttt gcgcgtcgca ataaccttag cgtctccgcc agtccgcagg
     6121 atatcggcgt gctgacgcgt aagctgtatg ccgcgtttga agcattacca ggtaaagtga
     6181 cgctggtaaa cccgcagatt tcacccgatc tctcggaacc gaatctgacc tttatttatg
     6241 tgccgccggg ccgtgctaac cgttcaggtt ggtatctgta taaccgcgcg ccaaatattg
     6301 agtcgatcat cagccatcag ccgctggaat ataaccgtta cctgaataaa ctggtggcgt
     6361 gggcatggtt taacggcctg ctgacctcgc gcacccgttt gtatattaaa ggtaacggca
     6421 ttgtcgattt gcctaagttg caggagatgg tcgccgacgt gtcgcaccat ttcccgctgc
     6481 gcttacctgc accgacaccg aaggcgctct acagcccgtg tgagatccgc catctggcga
     6541 ttatcgttaa cctggaatat gacccgacag cggcgttccg caatcaggtg gtgcatttcg
     6601 atttccgtaa gctggatgtc ttcagctttg gcgagaatca aaattgcctg gtaggtagcg
     6661 ttgacctgct gtaccgcaac tcgtggaacg aagtgcgtac gctgcatttc aacggcgagc
     6721 aatcgatgat cgaagccctg aaaactattc tcggcaaaat gcatcaggac gccgcaccgc
     6781 cagatagcgt ggaagtcttc tgttatagcc agcatctgcg cggcttaatt cgtactcgcg
     6841 tgcagcaact ggtttctgag tgtattgaat tgcgtctttc cagcacccgc caggaaaccg
     6901 ggcgtttcaa ggcgctgcgc gtttctggtc aaacctgggg gttgttcttc gaacgcctga
     6961 atgtatcggt acagaaactg gaaaacgcca tcgagtttta tggcgcgatt tcgcataaca
     7021 aactgcacgg cctgtcagtg caggttgaaa ccaatcacgt caaattaccg gcggtggtgg
     7081 acggctttgc cagcgaaggg atcatccagt tctttttcga agaaacgcaa gacgagaatg
     7141 gctttaatat ctacattctc gacgaaagca accgggttga ggtgtatcac cactgcgaag
     7201 gcagcaaaga ggagctggta cgtgacgtca gtcgcttcta ctcgtcatcg catgaccgtt
     7261 ttacctacgg ctcaagcttc atcaacttca acctgccgca gttctatcag attgtgaagg
     7321 ttgatggtcg tgaacaggtg attccgttcc gcacaaaatc tatcggtaac atgccgcctg
     7381 ccaatcagga tcacgatacg ccgctattac agcagtattt ttcgtgatga acgtgccgga
     7441 aagcgaggct tatccggcat gcaatcttag cggaaactga ctgtttcacc cgcctgctgc
     7501 gtcgccgcct gttccagcaa atcccagaag gtttcgccgc tgcgatcgca aatccactca
     7561 tcgcctttca gatcaaaatg gtagccgcct tgtttggttg ccagccatac ctggtgcagc
     7621 ggttcctgtc ggttgataat gattttgctg ccgttttcaa aggtaatggt cagtacgccg
     7681 ccgttgattt cgcagtcgat atcgctgtcg ccatcccagt cgtccaggcg ttcttcaatg
     7741 gtcagccaga gttgatcagc caggcgatga aattcactgt cgttcattgt tgtatcctgt
     7801 ttttaagtga tggcggcagt atagcggcat ggggtcaggg cttcaaagtt tgcacctctg
     7861 cggctgcgtt ccggcacgat tcatccgtca ccggaataat gatgtctcag tgtagcgaaa
     7921 gatttgtctc ttcattaggg cgcagttaca ccacgccttt ccctgtttct ggtaaacatt
     7981 atgatcaggt ttaccgagcg agcatcctca cgctgacgga actaaaaaag ccaacaaaca
     8041 aactacattg cgatagtgca taaagccatc ctggcgcgag gtgccgatca cgaaactacc
     8101 agcaaaacat aaatccccac gagtaagcgt tatactcgca gcatttcctc acttttcaga
     8161 cttcataaag agtcgctaaa cgcttgcttt tacgtcttct cctgcgatga tagaaagcag
     8221 aaagcgatga actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat
     8281 tacttactct cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg
     8341 cagataaaaa tgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc
     8401 cggataaaaa cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag
     8461 tttctgtacc cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca
     8521 acgattttat ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc
     8581 gtcgcctggc ggatcggcac ctgggggtag ggtttgacca gctgctggtg gttgagccgc
     8641 cgtatgatcc tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg
     8701 cgcagtgcgg caacggtgcg cgctgctttg cccgttttgt gcgtctgaaa ggactgacca
     8761 ataagcgtga tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg
     8821 atgatctggt ccgcgtaaat atgggcgaac ccaacttcga accttccgcc gtgccgtttc
     8881 gcgctaacaa agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg
     8941 gcgtggtgtc gatgggaaat ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg
     9001 cggtagaaac gcttggtcct gttctggaaa gccacgagcg ttttccggag cgcgccaata
     9061 tcggttttat gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg
     9121 caggagaaac ccaggcctgc ggcagcggcg cgtgtgcggc ggttgcagta gggattcagc
     9181 aaggtttgct ggccgaagaa gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct
     9241 ggaaaggtcc gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat
     9301 ttattcatct atgaagcaac caggggaaga actgcaggaa acactcacgg agcttgatga
     9361 ccgggcggtt gtcgattatc tgattaaaaa tcctgagttt tttatccgta atgcgcgcgc
     9421 agtagaagcg atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca
     9481 catggcccgc gcacgtaatc atattcatgt tctggaagag aacatggcgc tgttgatgga
     9541 acaggctatc gccaacgaag gcctgtttta tcgcctactc tacctgcagc gcagtctcac
     9601 cgccgccagc agtctcgacg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg
     9661 cctggcaggt gcgagtctgc gcctgtttcc ggatcgctgg cgcttaggtg cgccgtcgaa
     9721 ccacactcat ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg
     9781 gcaggaacag cactatcttg ggccgcttaa cggaccagag ctgctggtgg tgctaccgga
     9841 agcgaaagcg gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggtgt
     9901 cgtgctgttt accagtcgcg atgccagtca ctatcaacaa gggcaaggaa cgcagttact
     9961 tcatgaaatt gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc
    10021 gatttacaca ccgatgtaga acgctaccta cgttatctga gcgtggagcg ccagcttagc
    10081 ccgataaccc tgcttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa
    10141 aacggcctgc aaagctggca gcaatgtgat gtgacgatgg tgcgcaattt tgctgtacgc
    10201 agtcgccgta aagggctggg agcagcaagc ctcgcgttac ggctttctgc gctacgtagc
    10261 ttttttgact ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg
    10321 gcaccgaaag cgccgcgtca tctgccgaaa aacatcgacg tcgacgatat gaatcggctg
    10381 ctggatattg atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg
    10441 tacggcgcgg gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg
    10501 gagtctggtg aagtgtgggt aatggggaaa ggcagcaaag agcgccgcct gccgattggt
    10561 cgcaacgctg tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa
    10621 gacgacgcgc tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa
    10681 cgctttgccg aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta
    10741 cgtcactcgt tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag
    10801 ctgctgggtc atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac
    10861 cttgcctcgg tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt
    10921 accggccttt ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata
    10981 accgtccggt aattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc
    11041 cggcgctgcg cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag
    11101 cggaacccga gatttatcac gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga
    11161 tgctcgacgc cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta
    11221 actttgccaa atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac
    11281 agctggcgaa gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt
    11341 ttggtttggg ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac
    11401 cgttcagcga tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct
    11461 tacatgttgg ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg
    11521 cttgttggat cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc
    11581 cgcatctgga aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt
    11641 atatataccc agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc
    11701 ggcggtgcca atggacgttt cttacctgct cgacagcctt aatgacaaac agcgcgaagc
    11761 ggtggccgcg ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg
    11821 cgtactggtg catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat
    11881 tatggcggtg acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcgggcaact
    11941 gatgggcacc agccagggcg gcatgtgggt cggcaccttc cacgggctgg cgcaccgcct
    12001 gctgcgtgcg caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga
    12061 ggaccagctg cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg
    12121 gccgccgcgc caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca
    12181 tcatattcaa agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta
    12241 tcaggaagcg tgtgaccgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca
    12301 cgaactgtgg cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat
    12361 cctggtggac gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc
    12421 gggcgacacc ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg
    12481 cggggcgcag gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaactat
    12541 tcgtctggag caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat
    12601 tgaaaacaat aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc
    12661 tatttccctc tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat
    12721 caaaacctgg caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa
    12781 cgcccagtcg cgtgtactgg aagaagcact gttgcaggcc agtatgccgt accgtattta
    12841 cggcgggatg cgcttcttcg aacgccagga aatcaaagac gcgctctcgt atctgcgtct
    12901 gattgccaac cgtaacgacg acgcggcgtt tgagcgcgtg gtgaatacgc caacgcgggg
    12961 tattggtgac cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact
    13021 ctggcaggca tgtcgtgaac tgttgcagga aaaagccctc gccgggcgag ctgccagcgc
    13081 cttgcagcga tttatggaat taatcgatgc cttagcgcaa gaaactgccg atatgccgct
    13141 gcatgtacag actgaccggg taattaaaga ctccggcctg cgcaccatgt acgagcagga
    13201 gaagggcgaa aaaggccaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg
    13261 ccagttcagc tacaacgaag aagatgaaga tttaatgccg ctgcaggcat tcctctccca
    13321 tgcggcgctg gaagcgggcg aggggcaggc ggatacctgg caggatgcag tgcagttgat
    13381 gacgctgcac tcggcgaaag gcctggagtt cccgcaggtg tttattgtcg gtatggaaga
    13441 gggtatgttc ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg
    13501 tctggcctac gttggtgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac
    13561 ccgccgtctg tatggtaaag aggtttacca tcgcccgtcg cgctttatcg gcgagctgcc
    13621 ggaagagtgt gtggaagagg tgcgcctgcg cgctacggta agtcgtccgg tcagccatca
    13681 acggatgggg acgccgatgg tcgagaacga cagcggctac aagctcggtc agcgtgtacg
    13741 ccacgctaag tttggtgaag gcaccatcgt caatatggaa ggcagcggcg agcatagccg
    13801 tttgcaggtg gcattccagg gccagggtat taaatggctg gtggcggcat acgcccggct
    13861 ggagtcggtg taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat
    13921 tcatattcaa taaattgcaa cgtcatgtag gccggatagg gcgtttacgc cgcatccggc
    13981 atctgcgcca tcttcagtat ctgacacaaa actatcgttt taacctttcc gctctacgga
    14041 aatcatggtg acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg
    14101 tgaaatcgtg ccatcaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca
    14161 ggcaaaatca taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt
    14221 attaatccca acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt
    14281 aattacactg ttttccatca acattggccc tgactgcacc gcaaactgaa tctctttact
    14341 ggttttgaag gcatccagac gaacgatgcc gactttatct cccgcgacat aaaacacgcc
    14401 gccaggacgg ataaagaaat tcccttcacc tgaagcgaga tttaacgcca ccttctgctg
    14461 accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc cgccgttcat
    14521 cgccatctgc acctgaccct gactattcat atccgccagc agagcatgta aagttcccca
    14581 cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag gattaacggt
    14641 atacgcctgt acggtcagcg tcggatctga gagtgcgcaa tcatcagcgg caacagcaaa
    14701 caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga ttcaaggtga tcattccttt
    14761 accaatgagt agccgatgcg ccattatagg tcctggatgt tggatttttt tatcctgtta
    14821 gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg tgttctgttg
    14881 cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct aagaggacat
    14941 tcgccttgga cacacccagt agatactggc tcactatcct gtcattcagg atcaactcct
    15001 aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat ttttcccgtc
    15061 gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg cccaccgaac
    15121 tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca actggaaaat
    15181 aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc agtatggatt
    15241 gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact tggccagagc
    15301 ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cacgtttctt tgaagacgat
    15361 gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca cgccggtaac
    15421 tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga gcgtgaactg
    15481 cctgcattcc gtctgtatcg tatgcgcgcc cgtagccagt caatggtgga cggtaacgcc
    15541 tacgagctgc tgttggatct gttcgaaacc aaaatcgaac agctggcaga tgaaattgaa
    15601 aatatctaca gcgatctgga gcagttgagc cgggtaatta tggaagggca tcagggcgat
    15661 gagtacgatg aggcgctctc cacgctggcg gaactggaag atatcggctg gaaagttcgc
    15721 ctgtgtctga tggataccca gcgtgcgctc aacttcctgg tacgcaaggc gcgtttaccg
    15781 ggtgggcaac tggagcaggc gcgtgaaatc ctgcgagata tcgaatccct gctgccgcat
    15841 aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcgatggg ctttatcaac
    15901 atcgagcaga accgcatcat caaaatcttc tcggtggtat ccgtggtatt cctgccgccg
    15961 acgctcgttg cttccagcta tgggatgaac tttgagttta tgccagaact gaagtggagt
    16021 ttcggctacc caggcgcgat tatctttatg atcctcgcgg gcctggcacc gtatctgtac
    16081 tttaagcgca agaactggct gtaatatgag tgccggataa agctattttt ttatccggct
    16141 tatttctaaa aattgtctta tttaaagtca tgcggattga aaactcgctc tattttattt
    16201 tcttgtgtta attgatactc agtaaaatcg ttaataccaa tatgagtgtt acgataaggg
    16261 tattcattaa taaccattaa gtaatttttc acgatgcaat tatcattgct cttaattgtc
    16321 gggaaacagt agagatcttc atacggccct tcgatatatt ttttatagtc agtgaagcca
    16381 ttaccgatat caatgacata ccaggtatgc caactacctt ctggtagggg atgttgattt
    16441 ttaatttcat tctgatgctt cagataccaa tttattttat cgctatcagt ccatggcaga
    16501 tgatcaacga ccagaaagac tgaattgcca gcatcagagt atgtatacaa aatctgtaca
    16561 ggacgcaatg tccataaatt atagatgcag catagaagaa ataaacataa tataattttc
    16621 tggagtttgc ttttcataga taattttcga ttctttcgat agtgttaaaa ttggtgaaaa
    16681 aaggtttgaa agcaaagtct ttatgccgtt gtaaaaagaa c
//
LOCUS       DAPGWD010000092        16531 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPGWD010000092 DAPGWD010000000
VERSION     DAPGWD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.093"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(235..2196)
                     /gene="topB"
                     /locus_tag="RHX88_002894"
     CDS             complement(235..2196)
                     /gene="topB"
                     /locus_tag="RHX88_002894"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDV2231563.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2201..3244)
                     /gene="selD"
                     /locus_tag="RHX88_002895"
     CDS             complement(2201..3244)
                     /gene="selD"
                     /locus_tag="RHX88_002895"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDV2231564.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3361..3912)
                     /locus_tag="RHX88_002896"
     CDS             complement(3361..3912)
                     /locus_tag="RHX88_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDV2231565.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4073..5929
                     /gene="sppA"
                     /locus_tag="RHX88_002897"
     CDS             4073..5929
                     /gene="sppA"
                     /locus_tag="RHX88_002897"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDV2231566.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6096..7112
                     /gene="ansA"
                     /locus_tag="RHX88_002898"
     CDS             6096..7112
                     /gene="ansA"
                     /locus_tag="RHX88_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDV2231567.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7123..7764
                     /gene="pncA"
                     /locus_tag="RHX88_002899"
     CDS             7123..7764
                     /gene="pncA"
                     /locus_tag="RHX88_002899"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDV2231568.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7925..8197)
                     /locus_tag="RHX88_002900"
     CDS             complement(7925..8197)
                     /locus_tag="RHX88_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDV2231569.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8239..8652)
                     /gene="msrB"
                     /locus_tag="RHX88_002901"
     CDS             complement(8239..8652)
                     /gene="msrB"
                     /locus_tag="RHX88_002901"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDV2231570.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8994..9989
                     /gene="gapA"
                     /locus_tag="RHX88_002902"
     CDS             8994..9989
                     /gene="gapA"
                     /locus_tag="RHX88_002902"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV2231571.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            10073..10957
                     /locus_tag="RHX88_002903"
     CDS             10073..10957
                     /locus_tag="RHX88_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDV2231572.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(11008..11862)
                     /gene="yeaE"
                     /locus_tag="RHX88_002904"
     CDS             complement(11008..11862)
                     /gene="yeaE"
                     /locus_tag="RHX88_002904"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDV2231573.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11952..12698)
                     /gene="mipA"
                     /locus_tag="RHX88_002905"
     CDS             complement(11952..12698)
                     /gene="mipA"
                     /locus_tag="RHX88_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDV2231574.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            13134..15068
                     /gene="yeaG"
                     /locus_tag="RHX88_002906"
     CDS             13134..15068
                     /gene="yeaG"
                     /locus_tag="RHX88_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDV2231575.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15181..16464
                     /locus_tag="RHX88_002907"
     CDS             15181..16464
                     /locus_tag="RHX88_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDV2231576.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         4074 a         4280 c         4067 g         4110 t
ORIGIN      
        1 gccttatttt ctttaatcat ccgttcaaaa ttactattaa atatttccca gccattaaaa
       61 gaatacttct cgctcccagg atggttttgt aataaaactt ttttcatcac attccctgtt
      121 attacatact ttagaggcga taattttatc catgcaaaaa aaatatccaa cgaattttct
      181 tgatccgctg gatatttcag aatatgactc gaatagcacg aaagattcgc tcgcttacgc
      241 tatcgccccg cttccggctt catctgctgg cggacttttt ttcgcactac gtttacgcgg
      301 tgcagccttt ttcttatcag cactgccacc actgcccgga gccacaatgc cgcgaaactg
      361 ccgcaccgga gtacgtttgg cttgatcaat aagctgatat agcgtcccca ccagcggctg
      421 cataaagtcc tgatagcgac actgcttttc gctgatttgc gtcagcaccg attcccagtg
      481 cgcggtcatg tccggtcgcg tagccatttc tggcagcgaa tggaataacg cttttccggc
      541 gtcggtggag tggatatagc gccctttttt ggtgaggaaa ccacgcttga acaacagttc
      601 aataatcccg gcacgcgttg cttctgtccc cagaccatcg gtcgcacgaa ggatcttttt
      661 cagatctttg tcctgcacaa agcgcgcaat cccggtcatc gccgaaagca gtgttgcatc
      721 ggtaaaatgg cgcggcggct gggtttgccg ctctaccact tcaccttttt cacacagcaa
      781 ctcatcgcct ttcgccacca caggcaatgg cgtgccgtcg ttttcttcat cgcgctcttt
      841 gctgcctaac agcgcgcgcc aacctgcttc agcaagaaaa cgcgctttag cgacaaattt
      901 gcctttggca atgtccagtt cgataacaca cttgcggaac accgcatccg ggcagaactg
      961 catcagatac tgacgggcaa tcaggttata gaccttcgct tcgttctccg tcaggttgat
     1021 cgcagaactc cgtgcggtcg gaatgatggc gtggtgcgca tcgacctttt tgtcatccca
     1081 acagcggttg cgtatatctg gatctaccac tggctgcggc aacagatctg gtgcatgaac
     1141 gctgatggca ttcatcaccg cgtggcgtcc ggcaaaatgt tcttctggca aatagcgaca
     1201 atcggaacgc ggataagtga ttaacttgtg tgtttcgtac agcttctggc agatatcaag
     1261 cacgttctgc gcgctcagac caaagcgttt tgccgcttca atctgcaacg ccgaaagcga
     1321 aaacggcagc ggcgcggatt ctgattcccg tttatcgtta tagctggtga caatagccgg
     1381 ttgaccacta atgcggttaa ccacatgctc cgccagtgga cgatgtaaca agcgcccttc
     1441 ttcatcctgg tacggttcac acgcttcgct cggttgccag atagcggtaa accgctcatc
     1501 ggcaggtgtc acgatatgcg ctttgacttc aaagaagtct ttcgccacga agttttcaat
     1561 ctcttcatcg cggcgcacca ccagcccgag cacgggtgtc tgcacgcgtc ccacggaaag
     1621 tacgccctga taaccggcat tgcgaccgag aatggtatac gcacgggtca tattgatgcc
     1681 gtacagccag tcggcacgcg ctcgcgccag cgcagaaacg cacaatggca caaactcact
     1741 gttggaacga agacggtcga tcgcccgctc aaccgcctgc gggttcaggt cgtttatcaa
     1801 gcaacgctgc acctgctggc gcttttccgg tgccagttgc agatagtcca gcacttcatc
     1861 caccagcaat tgcccttcac gatccgggtc cccggcgtga acgatttcgc tggcttcatg
     1921 caggaagcgt ttgatgacgt taagttgttt ggtcacggag ggtcggggct gtaattgcca
     1981 cttttccggg acaatcggca aatccgcaag attccagcgc gcatagcggc tgtcgtaggc
     2041 gtctggctgc gcctgctcaa gcaggtgacc gatacaccag gtcaccacct gaccattacc
     2101 gcactcgata aagccatcgc ctttacggtg cggtttgggc aggacatcag caatggcgcg
     2161 cgccagactc ggtttttcgg caataaacaa ccgcattgaa ttaacggatc tcaaccatgg
     2221 cacgaccgcc gcgcgctggc accagttcgc caatcgccgt cagttcaatg ccaaactcgg
     2281 cggctgtagt tttaacctca ttttctgctt ccggcatgac cgccagcagc aaaccgccag
     2341 aagtttgcgg atcgcacagc agatcgcgca cttcacgcgg catttcaccc atcagatgac
     2401 cgtagctggc aaagttacgt tcggtgccgc caggcactgc gcccaactta atgtactctt
     2461 caacccccgg caattttggg atcgcgtcat agtcaacgcg cgcctgcaca cctgcgccct
     2521 gacacatttc gctcaaatgg cccagcagac caaaaccagt aacgtcggtc atcgctttta
     2581 cgccttcgat gttggcaaag gacgcacctg cgatgttcat ccggcacatc acttccgtcg
     2641 ccagtccctg atgttctggt ttcaacagtg attttttctc agccgtggta agaacgccga
     2701 tccccagcgg tttcgtcagg aacagtttgc atccggcttg tgcggtactg tttttcttca
     2761 cccgctcggt cggtacgatc cccgttaccg ccagaccaaa aatcggctcc ggcgcatcga
     2821 tggagtgacc gccagccagc gcaatacccg cctgacggca tgcatagcgt ccaccttcgg
     2881 ttacttcgcg ggcaatttct ggggaaagtt tgttaatcgg ccagccgagg atcgcaatcg
     2941 ccataatcgg tttgcccccc atcgcgaaga tatcgctgat ggcgttagtc gccgcaatgc
     3001 ggccaaaatc gaaaggatta tcaacgatcg gcataaagaa gtcggtggta ctgataacgc
     3061 tggtgccatt gcccagatcg tacaccgccg catcgtcgcg ggtctcatta cccacaagca
     3121 aattcggatc aacaaacttc gcctgctcac tgtgcaggat ggtttccaac acttttgggg
     3181 aaattttaca gccgcaacca gctccgtggc tgtattgggt caaacgaatc gagttctcgc
     3241 tcatggacat ctcctgtcaa tgcaatccgg gtatggtaac cctcattccg tgaagtgata
     3301 agtgagagtg tctgaattcc tgcgcctttg ctcacaatcc agacagtttc gcgacaatta
     3361 tcagaaataa gttacaaacg gcgtcgggtc cgggacgtta atcgacgtag atgctttcaa
     3421 ctgcggcgtg ccgaggtaga gaaaaccgac aattttatcc tgctcacggc aaccgaatgc
     3481 ttcacgcact accggacttt cagttaatgc gccactgcgc cagatgccgc caaatccttg
     3541 ggcaacggct gccatttgca tcgccatgac cgcgcatccg gcagacattt cctgttccca
     3601 tcgcgggact ttatgatttt cttcgcattt cgccaccacc gtgatgatga gcggtgcgcg
     3661 gaacggcgca ttacgggctt tgtcgatagc tttgtcatca ctaccggcag caatcgcccc
     3721 ctgttccagt acggcgctga aacgctcgcg cccttcccct tcaatcacaa aaaaatgcca
     3781 cggttgcatg gacttatggt ccggcgcacg catacccgca cgcaggatgt tttgcagttg
     3841 ttcacccgtt ggcgcgggtt cagccaggcg ggaggcgcta cggcgattga tcaatagttc
     3901 gagtgcatcc atttgattaa ctcctgtcgt gatatttatt cacaaaatta acacgagagt
     3961 ggattttgtt acagcacagt ccgcaattcc tgctgacaag tgccggttgg gtcattacga
     4021 taaccacatc tattgcgcct gtgacaggtg tgaccttaag ttgggagaat acatgcgaac
     4081 cctttggcga tttattgccg gattttttaa atggacgtgg cgtctgctga atttcgtccg
     4141 tgaaatggta cttaacctgt tctttatttt cctcgtactg gttggtgtgg ggatctggat
     4201 gcaggtcagt ggtggtgatt cgaaagaaac ggccagtcgt ggcgcactgc tgctggacat
     4261 ttctggtgtg atcgtcgata aacccgacag ttctcagcgg tttagtaaat taagccgcca
     4321 gctgcttggt gccagttccg atcgtctgca ggaaaactca ctgtttgata tcgtcaacac
     4381 cattcgccag gcgaaggacg accgcaatat caccggtatc gtgatggatc tgaaaaactt
     4441 cgcaggcggc gaccagccgt ctatgcagta catcggcaaa gccctgaaag agtttcgtga
     4501 cagcgggaaa ccggtttatg ccgttggcga gaactacagc caggggcaat attatctcgc
     4561 cagtttcgcc aataaaattt ggctgtcacc gcaaggcgtg gttgatctgc acggttttgc
     4621 taccaacggt ctgtactaca aatcgttgct ggataagctg aaagtttcca cccatgtgtt
     4681 ccgcgtgggt acgtataaat ctgccgttga accgtttatt cgtgatgata tgtcaccggc
     4741 agcccgcgaa gctgacagcc gctggattgg tgagctgtgg caaaactatc tgaatactgt
     4801 tgccgctaac cggcagatcc ctgctcagca ggtattccct ggcgcgcaag ggttgcttga
     4861 gggtttaacc aaaaccggtg gcgataccgc gaaatatgca ctggaaaaca agctggtcga
     4921 tgcactggca tcgagtgccg aaatcgaaaa agctctgacc aaagagttcg gctggagtaa
     4981 gactgataaa aattatcgcg ccatcagtta ttacgattac gcattgaaaa cgccggcaga
     5041 taccggtgac agcatcggtg tcgtcttcgc taatggcgca attatggatg gcgaggaaac
     5101 tcaggggaat gttggcggtg ataccactgc ggcacaaatc cgcgacgctc gccttgaccc
     5161 gaaagtgaaa gcgattgtcc tgcgtgttaa tagcccaggc ggcagcgtta ccgcgtctga
     5221 agtgattcgc gctgaactgg cagcagcccg ggcagcgggt aagcctgtgg ttgtatcgat
     5281 gggcggcatg gcggcatctg gtggttactg gatttccacg ccagctaatt acattgtggc
     5341 taaccccagc accctgaccg gttctatcgg tatcttcggc gtgatcacca ccgtagaaaa
     5401 tagtctggat tcgattggtg ttcatactga tggtgtctca acttcaccgc tggcggatgt
     5461 ttctatcacc agggcactgc cgccggaagc gcagcagatg atgcaattaa gcattgagaa
     5521 tggctataaa cgctttatca cgctggttgc tgatgcgcgt cattcgacgc cggagcaaat
     5581 tgataaaatc gcccagggcc acgtctggac cggtcaggat gcaaaagcta acgggctggt
     5641 cgatagtctc ggggatttcg atgatgcggt tgccaaagca gcagagctgg caaaagtgaa
     5701 acagtggcat ctggaatact acgttgatga accgaccttc ttcgacaaag tgatggacaa
     5761 catgtctggt tctgtccggg caatgttgcc agatgcgttc caggccatgt tacctgcacc
     5821 gcttgcctcg gtagcctcta ccgttaaaag tgaaagcgac aagctggccg cgtttaacga
     5881 cccacaaaac cgttatgcgt tttgcctgac ctgcgccaac gtgcgttaag tcttgtactg
     5941 agtggccgac agatcgtcgg ccacattatt ttttacctcg acgaatcctc ttcccgctgt
     6001 ctcgccccat atttccttat ccgcatagta tcaggtgcgc tcctcctgcc tcacgtatat
     6061 acttttgctc tttcgatatc attcatatca atatcatgca aaagaaatca atttacgtcg
     6121 cctacacggg tgggaccatc gggatgcagc gttccgagca gggttatatt ccggtgtcag
     6181 gtcatctaca acgccaactg gcgctgatgc cggaattcca tcgcccggag atgccagatt
     6241 tcaccattca tgaatatacg ccgttgatgg attcttctga tatgacgccg gaagactggc
     6301 agcatattgc tgaagatatt aaagcgcact atgacgacta tgatggtttt gtcattctac
     6361 acggtaccga cacgatggcg tataccgcct ctgcgctgtc gttcatgctc gagaatctcg
     6421 gtaaaccggt cattgtgaca gggtcacaaa tcccgctggc tgagttacgc tctgacggac
     6481 aaattaatct gctgaatgcg ttgtacgttg cggcgaatta tccgatcaac gaagtaacgc
     6541 tctttttcaa taaccgattg tatcgcggca accgcactac caaagcccat gccgatggtt
     6601 ttgatgcgtt tgcctctcca aaccttcctc cgttactgga agctggtatc catattcgtc
     6661 gtttgaatac gccacccgcc ccgcacggtg aaggggaatt aatcgttcat ccaatcacgc
     6721 cacaaccaat tggcgtagtg acgatttatc cagggatttc tgctgacgtc gtgcgcaatt
     6781 ttctgcgcca accggtgaaa gcattgattc tgcgctccta tggcgtgggt aatgcgccac
     6841 aaaacaaagc cttcctgcag gaattacaag aagccagcga tcgcggtatt gtggtggtca
     6901 acctgacaca atgtatgtcc ggtaaagtga atatgggtgg ttatgccacc ggtaacgccc
     6961 tcgcccatgc cggcgttatt ggcggtgcag atatgactgt agaagccacg ctaaccaaac
     7021 tgcattacct gctgagccag gaactggata ctgaaaccat tcgcaaggcc atgagccaaa
     7081 acctgcgcgg cgaactgacg ccggatgatt aaggagactg caatgcccca tcgcgccctg
     7141 ttactggtcg atttacaaaa tgatttctgt gctggtggcg cgctcgccgt gccggaaggt
     7201 gacagtacgg tagatgtcgc taaccgcctt attgactggt gccagtcgcg cggagaagcg
     7261 gttatcgcca gccaggactg gcatccggcg aatcacggca gttttgccag tcagcacggt
     7321 gtagagcctt atacgccagg ccaactcgac ggtttgccac aaaccttctg gccagatcac
     7381 tgtgtgcaga acagtgaagg cgcacaatta catccgttac tgaaccaaaa agcgatcgca
     7441 gcggtgttcc ataaaggcga aaatccttta gttgacagtt acagtgcctt ttttgataac
     7501 ggccgtcggc agaaaacctc tctcgatgac tggttacgcg atcatgaaat cgatgaattg
     7561 atcgttatgg gcctggctac tgactattgc gtgaagttta ccgtgctgga cgcgttacag
     7621 ttaggttata aggtgaacgt gattaccgat ggttgtcgtg gcgtgaatat ccagccccag
     7681 gacagtgcgc acgcgtttat ggagatgtca gcagctgggg caacgctata tacgctggca
     7741 gactgggaag agacgcaggg gtaatattag ttgaattcat ctctggccta catttcctca
     7801 atgacattgt aggccagata atacttgtca gtgttgcatt taacctactt gcagctaaaa
     7861 cacgacgtcc taacaagtat atttgccctc ccttaataac aggcttaaat actagataac
     7921 tcattcactt aaacatagca ataggctttg ctgagatacc aaaatcttct tttaactgct
     7981 gtttgctctt catgaccatc tgaccatttg tgtcaattgt catatgctgt gcttcggtat
     8041 tatggcgggc ttgccacagc atcaccagtt gcaggcaatt ctctttttgt tcctcggtca
     8101 acgcaacgcc atcaggccat ttccccagtt caacggcggt cgacaaacgc tggtatacct
     8161 caggcgtcat gctgttgata atgtcatcaa gattcataat ttttccgctc ctgtggaata
     8221 agttgctgaa tcgttttttc aaccgttgat ttcttcgccg ttttcgccat cagtgaagcg
     8281 taaagaagca gagttaacac aataacgttc gcctgttggt tgcggcccgt cgggaaagac
     8341 atgccccaga tgggcatcac agttaccgca acgaatttct atgcgctgca ttccatgtga
     8401 caagtctttg atataacgaa tggattcttc actcaccggt tcgtagaaac tgggccagcc
     8461 acagccggaa tcatacttgg tttcggaatg aaacagcggg gcatcgcaga tcaaacagtg
     8521 atatacgccg tcacgcttgt tatgcagtaa acgacccgta aatggcggtt ctgtcccatg
     8581 attctgcgtc acgtaaaact gcatctcgga caaatttttt ttcagttctt ctgccgaagg
     8641 tttattagcc atttgctcac atctcacttt aatcgtgctc acattacgtg actgattcta
     8701 acaaaacatt aacaccaact ggcaaaattt tgtcctaaac ttgatctcga cgaaatggct
     8761 gcacctaaat cgtgatgaaa atcacatttt tatcgtaatt gccctttaaa attcggggcg
     8821 ccgaccccat gtggtctcaa gcccaaagga agagtgaggc gagtcagtcg cgtaatgctt
     8881 aggcacagga ttgatttgtc gcaatgattg acacgattcc gcttgacgct gcgtaaggtt
     8941 tttgtaattt tacaggcaac cttttattca ctaacaaata gctggtggaa tatatgacta
     9001 tcaaagtagg tatcaacggt tttggccgta tcggtcgcat tgttttccgt gctgctcaga
     9061 aacgttctga catcgagatc gttgcaatca acgacctgtt agacgctgat tacatggcat
     9121 acatgctgaa atatgactcc actcacggcc gtttcgacgg taccgttgaa gtgaaagacg
     9181 gtcatctgat cgttaacggt aaaaaaatcc gtgttaccgc tgaacgtgat ccggctaacc
     9241 tgaaatggga cgaagttggt gttgacgttg tcgctgaagc aactggtctg ttcctgactg
     9301 acgaaactgc tcgtaaacac atcaccgctg gtgcgaagaa agtggttatg actggtccgt
     9361 ctaaagacaa cactccgatg ttcgttaaag gcgctaactt cgacaaatat gctggccagg
     9421 acatcgtttc caacgcttcc tgcaccacca actgcctggc tccgctggct aaagttatca
     9481 acgataactt cggcatcatc gaaggtctga tgaccaccgt tcacgctact accgctactc
     9541 agaaaaccgt tgatggcccg tctcacaaag actggcgcgg cggccgcggc gcttcccaga
     9601 acatcatccc gtcctctacc ggtgctgcta aagctgtagg taaagtactg ccagaactga
     9661 atggcaaact gactggtatg gcgttccgcg ttccgacccc gaacgtatcc gtagttgacc
     9721 tgaccgttcg tctggaaaaa gctgcaactt acgagcagat caaagctgcc gttaaagctg
     9781 ctgctgaagg cgaaatgaaa ggcgttctgg gctacaccga agatgacgta gtatctaccg
     9841 atttcaacgg cgaagtttgc acttccgtgt tcgatgctaa agctggtatc gctctgaacg
     9901 acaacttcgt gaaactggta tcctggtacg acaacgaaac cggttactcc aacaaagttc
     9961 tggacctgat cgctcacatc tccaaataag ttgagatgac actgtgatct aaaaagagcg
    10021 acttcggtcg ctcttttttt tacctgataa aatgaagtta aaggactgcg tcatgattaa
    10081 gaaaattttt gcccttccgg tcatcgaaca aatctcccct gtcctctccc gtcgtaaact
    10141 ggatgaactg gacctcattg tggtcgatca tccccaggta aaagcctctt ttgcattaca
    10201 gggcgcacac cttctctcgt ggaaacctgc gggtgaagaa gaagttctgt ggttgagcaa
    10261 caacacaccg ttcaaaaatg gcgtcgctat tcgcggtggc gtaccggttt gctggccgtg
    10321 gtttggtccg gcggcacaac aaggtctgcc tgcgcacggt tttgcccgca acctgccgtg
    10381 gacgctgaaa tcacatcgtg aagatgctga tggcgtagcg ctgacttttg aattgacgca
    10441 aagcgaagag acgaaaaaat tctggccgca cgactttacg ctgttagcgc atttccgcgt
    10501 gggtaaaacc tgtgaaatcg atcttgaatc acatggcgaa tttgaaacca cctctgccct
    10561 gcatacctac tttaacgtgg gtgatatcgc taaggtaagc gtcagtgggc tgggcgatcg
    10621 cttcattgat aaagtaaatg acgcgaaaga agatgtactg accgacggta ttcagacctt
    10681 ccctgaccgt accgatcgcg tgtatctgaa tccgcaagat tgcagcgtga ttaatgatga
    10741 agcgctgaat cgtattatcg ccgtaggcca ccagcatcat ctgaacgttg tcggctggaa
    10801 cccgggaccg gcgctttcag ttagcatggg cgacatgccg gatgatggct acaaaacatt
    10861 tgtttgtgta gaaacggctt acgcttcaga aacgcaaaaa gtgaccaaag agaaacctgc
    10921 acatctggcg caatccattc gcgttgcgaa acgttaattt gcgttaatgt tgtgtgccgg
    10981 gtgcgatgca tccggcacgt atcaacatca caccatatcc agcgcagttt ttccttttgg
    11041 tgccggatac gctttatcca gcatggttaa ttccgctgac gaaagttcga cctcaagcac
    11101 agccgcattc tgttggacat gggcaatcgt ggccgctttt ggaatcgcca tcacaccctg
    11161 atggctgatt acccacgcca acaatacttg tgccgcgctg atattgtgag catgtgcaat
    11221 ttcgttgact accgcgtttt ttaacagtcc attgcgcaac cgcccggcct gggctaatgg
    11281 actgtaagcc atcaccggca tctgctgttg ctggcaccag gggagtagat cgtactcaat
    11341 ccctcgtgaa ccgagatggt aaagcacctg attagtggca cactgatttc cccccggcag
    11401 ctgccagagt tcctgcatat cagcatgatc aaggttagaa acgccccagc ggcggatttt
    11461 tccctgggcg atcaattttt ccatcgctgc cacagtctct tcaaaagcga aactgccaga
    11521 ccagtgtaat aagtagagat cgagataatc agtattgaga cggcgtaaac tggcttcgca
    11581 tgcatttatc gctttttgcc cgccagcatt ccacggatag actttagaga cgagaaagac
    11641 gttatcgcgc agaccggtta atgcttcccc aaccaccttt tcggcaccgc catcggcata
    11701 catttcggcg gtatcaatga gggttaaacc gagctcaatg cccgcgcgta gtgcagcaac
    11761 ttctgttttg cgctgactgg catcttcgcc catataccat gttccctgcc ctatggctgg
    11821 cagtgagaca tcgccactaa attgaatcat tttttgttgc attgtttcct cccaggtatt
    11881 gcaccaccgt aatgcaaaac agggcgtgac gccctgtttt tatgcacaaa atgccctgga
    11941 aagatgcatt atcagaattt gtaggtgatc ccggtagaaa tcaggccagt ccaggattta
    12001 tccaccatcg ggctgtcagt aacttcatca gacagacggg tatagcgcgc ggtaccgtaa
    12061 acactccagt cgccgaggaa gttgtagctg gcgctcagct ccaggtaagg gctccagctg
    12121 tcgttcgggt tatagccacg cagaccgctg cgagcggact ctttgcgcga tacgccataa
    12181 tagtattcgt tctggttttc gctgttccac tgcacaccaa tacccggagt cacggtcagg
    12241 ccaccgttgg tgtaacgata caaccaggcc atatcccaga caatgccgtt gctgttatcc
    12301 agggtatcgc cagccagggt ggtacgcagg taaccgtact gggtaaagtg agcataagac
    12361 agaccagcca tcatggtgct cttacggtca tccaggtgac gcatttggtg atcgccactg
    12421 tctttcgctt tgaagtaaag cggcgaccag taagcggtaa ttgaaagttt atccgttgcg
    12481 tcattccaca ggtagtaacc accacctaag ccacggaacc agaagttatc gccttcatag
    12541 ttgattaccg gtactgggta aacatcggta tcgtaatctt tatatgggtg ctcaacgaca
    12601 cctacgcctg cgcccaggga aaatttacct tcagcgtgcg ctacgcctgc agacgttgcg
    12661 ataagcactc caagtgccag aagtttgagt ttggtcacaa ttaatcattc cttaaacaaa
    12721 tgtttagcgg gcgacaaagt ttacccgtca atacatcgaa acccaacctt tttacgtttt
    12781 cattttttaa agtaactgtt taattttcct gacgcggatg acaccgcgct tacagccaaa
    12841 tgaatttagc gttactggct accctggtct ttacattaat tatgcaaaat ttatggatga
    12901 gttgttgata tgccattgaa attaagaaag ccgtgcaggc aagttttcca tttgccatct
    12961 acgcttaatt ttgaaggtgt atcaccgggc acgttgttct catcgtcgat aaaatggcat
    13021 gagagttgct gtgttttagc aagagacgtc gttcagttta cctcttccgg gagcctctac
    13081 tattcatatg aacggctctt aacctgtgct aaaaaacgaa aggacggcat accatgaata
    13141 tattcgatca ctatcgccag cgatatgaag ctgccaagga cgaagagttc acactgcagg
    13201 agtttcttac cacttgtcgg caagatcgca gtgcttatgc caacgcggct gagcggctat
    13261 tgatggctat cggtgagcct gtcatggtcg atacagccca ggaacccaga ctttctcgac
    13321 tcttttctaa ccgggtcatt gcacgttatc cggcatttga agagttttac ggcatggaag
    13381 acgcgattga acagattgtc tcttatctga aacacgcggc tcaggggctg gaagagaaga
    13441 aacaaatcct gtatctgctg gggcctgtgg gtgggggtaa atcatcgctt gctgagcgac
    13501 tgaaatcatt aatgcagctc gtaccgattt atgtattgag cgccaacggt gagcgtagcc
    13561 cggtcaacga tcatccgttc tgtcttttca atccgcagga agatgctcag attctggaaa
    13621 aagagtatgg cattcctcgc cgttatctcg gcaccatcat gtcgccatgg gcggcaaaac
    13681 gcctgcatga atttggtggc gatatcacta agttccgggt agtgaaggtc tggccgtcaa
    13741 ttctgcaaca aattgctatc gccaaaacgg aacccggtga tgagaacaac caggacatct
    13801 ccgcgctggt tgggaaagtc gatattcgta aactcgaaca ctacgcgcag aatgacccgg
    13861 acgcctacgg ctattccggt gcgctgtgcc gcgccaacca agggatcatg gaatttgtcg
    13921 agatgtttaa agcaccgata aaagtgctgc atccgctgtt aaccgccact caggaaggta
    13981 actacaacgg gacggaaggt atctccgccc tgccgttcaa cgggattatt ctcgcccact
    14041 cgaacgaatc cgaatgggtc actttccgta ataacaaaaa caacgaagcc ttcctcgacc
    14101 gtgtttacat cgtgaaggtg ccgtattgct tacgcatttc cgaagagatc aaaatctacg
    14161 agaaattgct taatcacagt gaattgactc acgccccctg cgcccctggc acgctggaaa
    14221 cgctgtcacg tttttccatt ctttcgcgcc ttaaagagcc agaaaactcc agcatttatt
    14281 caaagatgcg ggtttatgat ggcgaaagtc tgaaagacac cgatcccaaa gccaagtcgt
    14341 atcaggaata tcgtgactac gccggtgtcg atgaagggat gaacggtctg tcgacgcgtt
    14401 ttgcgtttaa gatcctctcc cgcgtgttca acttcgatca tgtagaagtg gcggcaaacc
    14461 cggtccatct gttctacgta ctggaacagc agatcgagcg cgagcagttc ccacaagagc
    14521 aggcagaacg ctatctggag ttcctgaaag gttatctgat cccgaaatat gccgagttta
    14581 tcggcaaaga gatccagacg gcctaccttg aatcctattc cgaatatggg caaaacattt
    14641 tcgaccgtta tgttacctac gcggatttct ggattcagga tcaggagtat cgcgatccgg
    14701 ataccgggca gctgtttgac cgcgagtctc ttaacgccga gctggagaaa atcgagaaac
    14761 cggcggggat cagtaatcca aaagatttcc gcaacgagat tgttaacttc gtactgcgcg
    14821 ccagagcgaa taacagcgga cgcaatccga actggaccag ctatgaaaaa ctgcgcacgg
    14881 ttatcgagaa gaaaatgttc tccaataccg aggagctgtt gccggttatc tcgtttaacg
    14941 ccaaaacgtc aaccgacgag cagaagaaac acgacgactt tgtcgaccgt atgatggaaa
    15001 aaggctacac ccgtaaacag gtgcgtttac tgtgcgaatg gtatttgcgc gtacgtaaat
    15061 cgtcttaaca accctggccc ggtacacatg ttaccgggcc tacaacgaca gcgaaccgtg
    15121 ggcctgagaa gcggcaacac aggcgtagca tacagttggc aaatgtagta cggggggcat
    15181 atgacctggt ttattgaccg gcgtctgaac ggcaaaaaca aaagcatggt gaatcgccag
    15241 cgttttttac gccgttataa agcgcaaatt aaacagtcga tctccgaggc cattaataag
    15301 cgttcggtga ctgacgtcga cagcggcgaa tccgtatcca ttcccacgga agatattagc
    15361 gaaccgatgt ttcatcaggg gcgtggcggt ctgcgccacc gcgtgcatcc gggcaatgac
    15421 catttcgtcc agaacgaccg aattgaacgt ccccagggtg gcggcggagg ttccggcagt
    15481 ggtcagggcc aggccagcca ggatggtgaa ggtcaggatg aatttgtctt tcagatttcg
    15541 aaagatgagt atcttgatct gctctttgaa gatttggcct taccgaatct gaaacaaaac
    15601 caacaacgcc agctgaccga atataaaacg catcgggcgg gttataccgc taacggcgtt
    15661 ccggcaaata tcagcgttgt gcgctccttg cagaactcac tggcgcgacg cacagccatg
    15721 acggcaggca agcggcggga acttcatgca ctggaagaga atttggccat catcagcaac
    15781 agtgaacctg cgcagctgct ggaagaggaa cgtctgcgca aagaaattgc agaattacgt
    15841 gccaaaattg aacgcgtccc ttttattgac accttcgatt tacgttacaa gaactacgag
    15901 aagcggcccg atccctccag ccaggcagtg atgttttgcc tgatggacgt ttccggttca
    15961 atggatcaat ccactaaaga tatggctaag cgtttttata ttctgctgta tctgttcctc
    16021 agcagaacgt ataagaacgt ggaagtcgta tacatccgcc atcataccca ggcgaaagaa
    16081 gtcgatgaac atgagttttt ctactcgcag gaaacaggcg gcaccatcgt ttccagcgcc
    16141 ctgaaactga tggatgaggt agtgaaagag cgttataacc cggcacagtg gaatatttac
    16201 gctgcacaag catcggacgg cgataactgg gccgatgact ctccgctttg ccatgaaatc
    16261 ctggcgaaaa aattattacc tgttgttcgt tattacagct atatcgaaat tacccgtcgt
    16321 gcacatcaga cattgtggcg agaatatgag catctgcaat ctactttcga caactttgcg
    16381 atgcagcaca tccgcgacca ggatgatatt tatccggtgt tccgtgaact gtttcataaa
    16441 caaaatgcaa cagctaaaga ctaaaactat cagccaggtc attatcgcct ggctgatttt
    16501 tagcttactg taaattatct catttattac a
//
LOCUS       DAPGWD010000093        15836 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPGWD010000093 DAPGWD010000000
VERSION     DAPGWD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.094"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..338)
                     /locus_tag="RHX88_002908"
     CDS             complement(<1..338)
                     /locus_tag="RHX88_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231577.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLT"
     gene            complement(377..1606)
                     /locus_tag="RHX88_002909"
     CDS             complement(377..1606)
                     /locus_tag="RHX88_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2231578.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(1772..2134)
                     /gene="ssrA"
                     /locus_tag="RHX88_002910"
     tmRNA           complement(1772..2134)
                     /gene="ssrA"
                     /locus_tag="RHX88_002910"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2349..2831)
                     /gene="smpB"
                     /locus_tag="RHX88_002911"
     CDS             complement(2349..2831)
                     /gene="smpB"
                     /locus_tag="RHX88_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDV2231579.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            3002..3439
                     /gene="ratA"
                     /locus_tag="RHX88_002912"
     CDS             3002..3439
                     /gene="ratA"
                     /locus_tag="RHX88_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDV2231580.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            3429..3719
                     /locus_tag="RHX88_002913"
     CDS             3429..3719
                     /locus_tag="RHX88_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDV2231581.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(3781..4122)
                     /gene="bamE"
                     /locus_tag="RHX88_002914"
     CDS             complement(3781..4122)
                     /gene="bamE"
                     /locus_tag="RHX88_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDV2231582.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(4271..5932)
                     /gene="recN"
                     /locus_tag="RHX88_002915"
     CDS             complement(4271..5932)
                     /gene="recN"
                     /locus_tag="RHX88_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDV2231583.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(6018..6896)
                     /gene="nadK"
                     /locus_tag="RHX88_002916"
     CDS             complement(6018..6896)
                     /gene="nadK"
                     /locus_tag="RHX88_002916"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDV2231584.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            7019..7612
                     /gene="grpE"
                     /locus_tag="RHX88_002917"
     CDS             7019..7612
                     /gene="grpE"
                     /locus_tag="RHX88_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDV2231585.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(7667..8953)
                     /locus_tag="RHX88_002918"
     CDS             complement(7667..8953)
                     /locus_tag="RHX88_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV2231586.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVHIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8974..9765)
                     /locus_tag="RHX88_002919"
     CDS             complement(8974..9765)
                     /locus_tag="RHX88_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDV2231587.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            9932..11293
                     /gene="ffh"
                     /locus_tag="RHX88_002920"
     CDS             9932..11293
                     /gene="ffh"
                     /locus_tag="RHX88_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDV2231588.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            11430..11678
                     /gene="rpsP"
                     /locus_tag="RHX88_002921"
     CDS             11430..11678
                     /gene="rpsP"
                     /locus_tag="RHX88_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDV2231589.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            11697..12245
                     /gene="rimM"
                     /locus_tag="RHX88_002922"
     CDS             11697..12245
                     /gene="rimM"
                     /locus_tag="RHX88_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDV2231590.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            12276..13043
                     /gene="trmD"
                     /locus_tag="RHX88_002923"
     CDS             12276..13043
                     /gene="trmD"
                     /locus_tag="RHX88_002923"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDV2231591.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            13085..13432
                     /gene="rplS"
                     /locus_tag="RHX88_002924"
     CDS             13085..13432
                     /gene="rplS"
                     /locus_tag="RHX88_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDV2231592.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13509..13991)
                     /locus_tag="RHX88_002925"
     CDS             complement(13509..13991)
                     /locus_tag="RHX88_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV2231593.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(14007..15233)
                     /gene="dgcN"
                     /locus_tag="RHX88_002926"
     CDS             complement(14007..15233)
                     /gene="dgcN"
                     /locus_tag="RHX88_002926"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDV2231594.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            complement(15223..15741)
                     /locus_tag="RHX88_002927"
     CDS             complement(15223..15741)
                     /locus_tag="RHX88_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDV2231595.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         3942 a         3705 c         4107 g         4082 t
ORIGIN      
        1 ttagttaata actttaaatt taatgcttca ggattttcac tgagaaagtg aaaggcataa
       61 actaagtata cagctaaagt agccgttcca attgttggtc caatatcgcc ttgatcatga
      121 ggttcaaggt ttacattgaa cttgaagatt agccattcaa atccccaagt aaaaaaaaca
      181 actaatatca tggctgcgaa taatttcgcg atgttatctg ctgatggatt catcagaaca
      241 gattgtttct gaaaaaattc acaaagtgag agtccgaaaa aaataaaaaa aagtggtctg
      301 gaactaaatt tttcaaaaac tttgaatagg gtttccattt tctttacctt caggattata
      361 ttagctattc tgatacttag gctaccagag catttgatct ttgagcttga acgtaatcac
      421 tccaccattg catcaaactc tgtcgctcta tcagatattc tgcacgattg tatgctgcga
      481 taatttcatc ttttttcgag tgggcaagcg ctgcctcaag aacttcagct ctgaatttac
      541 cagactcctc tgccgctgtt cgtgcaatag aacgcatacc gtgagctaca agctcgcctc
      601 cgaaccccat tcggatgata gctgcgttgg ctgtttgttc atgcatatga ttaagaggcg
      661 cttttatgct ggggaaaacc cattctctat gcccacttat tgatttcatt aattcaagga
      721 tgcgcaaagc ttctttactc aaaggaactt tgtgaagctt tttcattttc atgaaatcag
      781 caggaatgtt ccaaatgctg ttggttgtat caatatcaga ccaccttgcg cgaacggctt
      841 cacccggacg aacccatgtc aacaattgcc attcaattag catacgtgtt tccaaccgga
      901 ttgacgcatt cgtcaaagat tccataaacc ttggcaattc gcttggggga agggcaggca
      961 tattttgctt ttttggttta ctgaatcttt gaccaaggtt gtcagccggg ttgaactcaa
     1021 taagttcttc agtagctgcc caccggaaga tttcattcag acgtgaaatg atacggcgta
     1081 gagtttccaa tacgcctcgt tgctcaatag gatcaaggtg ttgttttaag agcttaggtc
     1141 ggatctcatt gatagggaca ttacccagac cgggaaagac atttctctct aagctgcgcc
     1201 agatgtctgc tgcatggtct tgtgagatac ctgatgtctt taccttctca tctaaccatt
     1261 tccgcgctac ggcttggaga gtgtgctcag tagcactctt taatgccttc gccttatcgt
     1321 tgttatggat ttggggatca acaccatttg ccagaaagga gagatattca tcacgtaagg
     1381 ctctggctct tgcaagggta aggtgaggat atgtcccaag gctcattttg gttcttttct
     1441 tgctcactgg tactgcatac ctgaaatacc aatttttctt gcctcctttc gccaaaggag
     1501 cgattcgtag aatcagacca tcaccgtcaa acaagttgat ttctttatcg gctggcttgg
     1561 tgcttttgat ttcagtgtca gtgagcttct tagcgatttt tgccattttg ggaccctcgg
     1621 tttttggacc cttcttagtg ggtcccattc agggtgccat aactcgtagt tctcagcaat
     1681 tctcactgga cgacaataga cgtaaaaaag cccgcagagc ttgtgctgtg tgggcttagt
     1741 agacttcatt gaacttcaaa caactaaaaa gtggtggagc tggcgggagt tgaacccgcg
     1801 tccgaaattc ctacatcctc ggtactacat gcttagtcag tctttacatt cgcttgccag
     1861 ctgcggacgg acacgccact aacaaactag cctgattaag ttttaacgct tcaaccccag
     1921 gcagggcttc cacgcgatct cttttgggtt tgacctctct tgatccccgt cctaagagcg
     1981 gaggctaggg agagagggct ctaagcaggt tattaagctg ctaaagcgta gttttcgtcg
     2041 tttgcgacta ttttttgcgg ctttttacga ggccaaccgc ccctcggcat gcaccttggg
     2101 tttcgcaaat cccgtcgaat ccagaatcag ccccaatgtg taaaggtaag tataccagat
     2161 ttatgagcgc catgaccagc ctcaatggcg ttatcgttaa agatttagca cccatgtagc
     2221 ctgattttta ttcgattaag caatgggatg gcaacatttg tgtcggatgt gatagccaat
     2281 aagatgttca ttcgcgccgc cggagaggga ggcgcggtga ggaactggtc aataattgga
     2341 gtgcaggttt aacggtgggc gtttttcatg atacgtgctt tatccacctg ccattcgcgt
     2401 tctttgatat ctgaacgttt atcgtgctgt tttttacctt tggcgacgcc gattttcact
     2461 ttgcaccagg catttttcca gtacagggag agcgccacta cggtatagcc ttctcgattg
     2521 acgcgaccgt acaatgagtc cagttcgcgc tggttgagaa gtaacttgcg ggtacgggta
     2581 ggatcgcaca ccacatgcgt ggaggccacg gccattggcg tgatgttagc gccaaacaga
     2641 aatgcctctc cgtcacgcag aaggacgtag ctgtcgctga tattggcttt tcctgcgcgc
     2701 agggatttaa cctcccagcc ttgcagggca agtcccgctt cgaactcttc ttcgataaag
     2761 tattcgtgac gggcgcgctt gttaagcgcg atggtcgctg aaccaggttt atgtgctttt
     2821 ttcttcgtca taagcgtcgt gaatcatcgg taatctgaaa tcgaaaacac cccatatcaa
     2881 tcctgcgggg gacaaggctc tatcttagca tgaacccgat gttacccagc gccgggatag
     2941 cgtttttttt acagcaggat aaatgatatt atttgttgga tttttgttga tggaaattgt
     3001 tatgcctcag attagccgga ccgcactggt accctacagc gcggagcaaa tgtatcagtt
     3061 agtgaatgac gttcagtctt atcctcagtt tttgccgggt tgtaccggaa gtcggattct
     3121 ggagtccact cctgggcaga tgactgcggc ggtagatgtc tctaaggctg ggatcagcaa
     3181 aacgtttact acccgcaacc agttgaccag taaccaaagt attcttatga atctggtgga
     3241 tgggccgttc aagaaattga ttggtggatg gaagtttacg ccgctgagcc aggaggcgtg
     3301 tcgtatcgag tttcatctcg actttgagtt taccaataag ttgattgaac tcgcctttgg
     3361 tcgcgtgttt aaagagctgg cgtctaatat ggtccaggct tttacggttc gtgcgaaaga
     3421 ggtttacagt gccaggtaaa attgccgttg aggtggctta tgcgctacct gagaagcagt
     3481 acctgcagcg cgtgacgctg caggagggcg cgacggttga agaagctatt cgcgccagtg
     3541 gcttgctgga attgcgtacc gatatcgatt taactaaaaa caaagtcggc atttacagcc
     3601 gtccggcaaa actaagcgat agtgtgcatg atggcgatcg ggtggagatt tatcgccctc
     3661 tcattgccga tccgaaagag cttcgcaggc aacgagcaga aaaatcagcg aataaataac
     3721 agacagaaaa aaggtgctca ttgagcacct tttttaacgt ctttgagagc aactttatta
     3781 ttagttacca ctcagcgcag gtttgttatc aatattggtc aacacaccgc tactgttaaa
     3841 ggtcagcgtc agcgtttgct gcgttacacc ttcatgacct ggttgctggc ggaagacata
     3901 gaaccaggta ttcgtaccaa atggatcgga catcagcggt gtacccaatg cgtacgcaac
     3961 ttgttgttgc gtcatgccaa cacgtatttt ggatacgtcg ttagcggtca gatagttccc
     4021 ctggttgatg tcaggacggt aaaccactcg ctccagagtg gaacagcctg cggtcaacat
     4081 caatagtact gctgctgcag cagtcagcgt tttacagcgc atagtgattt gattcctttt
     4141 cgggcccgag cagtacgtgg ctcatctgta atatgccgat gataatagac ctttcatcac
     4201 tttaaaacct tttgctttcc ggtcttacgg cgttttgctg tttactctga ccgtgaagca
     4261 ggaaaaaagt ttacgctgca agcagttctt tcgcattcgc cagtgtatta cgtgtgactt
     4321 cactgccacc aagcaggcgc gccagctctt gtaaccgcgc ttttttattc agggattgca
     4381 tatgcgtttc tgtcatcgca ccatcggttt ctttgctgac aaaatagtgt tgatgaccac
     4441 atcccgcgac ttgtggcagg tgggtaacac acatcacctg agttgattcg ccgagttgac
     4501 gcagcagttt gccgacaact gccgctgttg gaccgctaat ccctacatcc acttcatcaa
     4561 aaatcagtgc cggggtttcc attttacgcg ccgtgatgac ctggattgcc agtgcgatgc
     4621 gggacaattc accaccggat gcgactttgg caataggctg cattggctga cctgggttgg
     4681 tggttacccg aaactcaata cgatcggcac cgtcagcgcc caggtgatgc tcgtcaaatt
     4741 taacatcgat cgtaaactgc ccatgcggca ttgagagcgc atgcatactg tcggtgatca
     4801 gctgtgcaag ttcttctgca tattgctggc gttgttggtg taatgcgcgc gcgatttcca
     4861 gtgcctgctg atgatgtttc gttaccgcca gcgcaagcgt ttcttgtgag tcggcctgat
     4921 cgtccagttg ctgctgttct tccagtagcg actggtaata ctgtggcaat gcctcagggc
     4981 tgacgtgatg tttacgtgcc agcgaaatct gttttgagat gcgctgttca agttcaaata
     5041 gtcggttggg atcgagatcc agacgatcgc agtagtggcg cagttcatcg ctggcttcag
     5101 caatctggat ggtagcttct tccagcatat caagtacgcc ggacagtttg ctgtccatgc
     5161 caatcaattc gctcaccagt tgtttagccg tgtaaagctg actttgcagg tttgcgtctt
     5221 caccgtcggc cattaatgcc aatgcattct ggctggtggt cagcaattga ccgctgttcg
     5281 ccagacgttt gtactcttcg tcgatttgtt caaactctcc gggctgcgga ttaaattcgt
     5341 taagttcttt taattggtat tgcagcagtt ccgcacgggc agcgcgttcc tgacttaact
     5401 gttgatgatg cgcgaggtca cggcagcttt gatgccacaa ctgataacgt gctgtcattt
     5461 cctggagtag agaggtttca ttggcatagc catcaagcag gaacttttgg tgctcgggtt
     5521 tggtgagtaa ctgatgagcg tgctgaccat ggatctgaat cagcaactga cccagttcgc
     5581 gcagttgtga cagaggaaca gctgtaccgt tgatgaaacc acgggagcga ccatcgctgc
     5641 tgatcacgcg acgaagcaaa cattcatgcc cgtcttcaag ctggttttct tccagccagc
     5701 gcagagccgc tggcgtatct ttcagagaaa aacgggcgca caggtcagcg cgagcagcgc
     5761 cggtacgcac catgtcggct tcagcgcgac caccgagaca aagaccgagg gcatctattg
     5821 caatagattt acccgcgccg gtctcgccag ttattacggt catgccgcta tgaaaatcaa
     5881 tctcaagctc acgaacgata gcaaagttgc tgatggtcag ttgtgccaac atagtcgttt
     5941 tcctgtatga aaaaccatta ctgttattgt gtacagtata aactggtttt atatacagta
     6001 aagaggctgg cgtaaaatta gaataatttt tttgaccagc cgagcttggt gcttaatgtg
     6061 ttgaaataac tgtaatcttt cggatgaatc agattcagat ggtaatcaca gcgacgaatc
     6121 aggacatctt caccttcctg aatcggcagt gctatctggc tgtcgcaact gatttccagg
     6181 tcgttacggc gatgcgaaaa acgcagacgg atcgtgctgc tgctgtttat gaccagtggt
     6241 cgtgctgaca acgtatgcgg gaacatgggc accagggtaa tcgcatccag agagggggtc
     6301 agaataggac cgcctgcaga gagggaatag gcggtggagc ctgttggcgt cgaaataatt
     6361 agtccatcag atcgctgaga aaacgcaaag atctcgtcga tatacacttc gaactcaatc
     6421 atatgcgcca ctttgcctgg atgaagcacc acttcattta tcgcggtgct gatgcgtttc
     6481 tggcaatctt gctgacagac ttgcgcttcc agcaaaaaac gtttctcgct gatgtagtgg
     6541 ccttccagca catcggctaa ctgttgctgg gcgttatcgg ggtcaaggtc agtcaggaaa
     6601 cccaggttgc cacggttgat tccaataact ttaatatcgt aacgggcgag tgtgcgtgcc
     6661 gcgcccagca tattaccgtc gccaccaacg actaccgcga gatcagctag ttgcccaatc
     6721 tccgcgagcg tgccagtttt cacattcttc agttgcagtt cgtgagcgat ttgttgctca
     6781 acgatgacct cgtaaccttt tgtgcacagc cagcggtaga gcatttcatg tgttgtcagt
     6841 gcagtggggt gccgtgggtg tcccacaatg ccaatacact tgaaatgatt attcattttt
     6901 ccgaggtcct tgttgcgaag attgatgaca atgtgagtgc ttcccttgaa accctgaaac
     6961 tgatccccat aataagcgaa gttagcgaga tgaatgcgaa aaaaacgcgg agaaattcat
     7021 gagtagtaaa gaacagaaaa cgcctgaggg gcaagccccg gaagaaatta tcatggatca
     7081 gcacgaagag attgaggcag ttgagccaga agcttctgct gagcaggtgg atccgcgcga
     7141 tgaaaaaatt gcgaatctcg aagctcagct ggctgaagcc cagacccgtg aacgtgacgg
     7201 cattttgcgt gtaaaagccg aaatggaaaa cctgcgtcga cgtactgaac tggatattga
     7261 aaaagcccac aaattcgcgc tggagaaatt catcaacgaa ttgctgccgg tgattgatag
     7321 cctggatcgt gcgctggaag tggctgataa agctaacccg gatatgtctg cgatggttga
     7381 aggcattgag ctgacgctga agtcgatgct ggatgttgtg cgtaagtttg gtgttgaagt
     7441 gatcgccgaa actaacgtgc cgctggaccc gaatgtgcat caggctatcg caatggtgga
     7501 atctgatgac gttgcgccag gtaacgtact gggcattatg cagaagggtt atacgctgaa
     7561 tggtcgtacg attcgtgcgg cgatggtaac tgtagcgaaa gcaaaagcgt aatttctgtt
     7621 ttcgtaataa ttcacggccc tgcatgcgaa tgcagggccg ttttcgttac tccgccacac
     7681 tctcgcgcag cggttttaca ggaaagactt ttacctgctt aatcatattg tcctgaacgt
     7741 cgagaatatc gatatcgtac tcgccaatat gcacgcgggt gccagcgaca gggatctctt
     7801 ccagagcttc aagaatgacg ccattaaccg tgcgggcatc gtcttcaggt agatgccagt
     7861 taaaggcttt gttgatttcc cgcacgttgg cagtgccatc aataatcacc gaaccgtcgt
     7921 tttgcggcgt gacctcttcg gcaagtgttg gcgacatcga cgtagtgaaa tcgccgacaa
     7981 tctcttccag aatatcttca accgtcacca gcccctgaat gtctccatac tcgttgacga
     8041 ccaggccgac tttctttttg ttgcgctgaa atttcaccaa ctgcgtgctg agcggcgtac
     8101 cttccgggac aaaatagatc tcgtccgcgg cgcgtagcat ggtttctttg gtgaactctt
     8161 ttttctccga catcaaccgc caggcttcac gcacacgcag catactgatg gcgtcgtcca
     8221 gcgaatcacg gtagagcacg atgcgcccgt gaggtgagtg ggagagttgg cgcagaatcg
     8281 atttccagtc atcgttgata tcaataccga taatttcact gcgcggcacc atgatgtcat
     8341 caacggtcat tttttccaga tcgagcaccg acagcagcat atcctgattg cgacgggaaa
     8401 tttgtgagcg cgattcatgc acgatagtgc gcaactcttc tttgctcaaa gagccgctga
     8461 ccacgatatc ggttttgatg cccatcatgc gcattaacat gcgggtgata gcattcagca
     8521 accagaccag cggcatcatc aaaatttgca gcggagccag cagaaaacta ctcggataag
     8581 cgactttttc cgggtacagc gcggcaatgg ttttcggcaa tacctcagca aataccagta
     8641 cgacaaaagt cagcacacca gtcgcaattg ccacgcccgc atcgccgtac aaacgcatcc
     8701 caacaatagt gccgagcgcg gaggcaagaa tattgaccag gttattgccg attaacacca
     8761 ggcttatcag gcggtctggc ttacgcagca atttttcgac gcgtttggcc gagcgattac
     8821 cctgtttcgc catatgtcgc agacgatagc ggttgagggt catcattccg gtttcggacc
     8881 cggaaaaata ggctgaaatg accaccatga tgatcagaat aatgatcaac gtagtagtag
     8941 aaatgtgttc caggggaaac tccttttctg ggtttagctg attaactgct ggacaattcg
     9001 gctgccgaag taggccagtg tcagaattac cgcgcccgca acgttaaacc agacgacgcg
     9061 gcgtccacgc catccttcat ggtaatgtcc ccacagcagc acaatataga caaaccacgc
     9121 cacgatagag agcacagcct tgtcgatatt ttccatgcta aataggttgt gcatgtagaa
     9181 caggccagtg caaagcgtga gcgttagcag caccacgcca atctgcgtga tgtggaacat
     9241 tttacgctcg atactcatca atggcggcat ttcctggtta aatgccagct tcttgttctt
     9301 cagttggtaa tcaatccacg ccagttgcag cgcgtacagg gcggcgataa ttagcgtggc
     9361 ataggaaaag agcgataagc caatgtgcac cagcatccca ggcgttgctt ccagatgggt
     9421 gatgtattca ttgggcatga aggtcgccag cgccaggttg ataagcgcaa aggcatagac
     9481 aatgggtagc agcaaccagc cacgattgcg agaagccaca atggtcatta ccgtacagat
     9541 catcaaactg accaatgaac caacgttcag caggctgagg ttttgtccgc tatcaccgtc
     9601 gggcaggatg cgggcttcca gagcgattgc gtggcagacc agcgcaatga ccgcagaaat
     9661 aatagccatg cgccgccagc cgccgttttt ttgcagcaga ccgggaacaa tcagcgcaag
     9721 actgacggag taggcgacaa gcgcgagcag agcaaaaacg ggcatagtga tgtcgacagt
     9781 ttgagcaata agaaagagaa gcagtatagc gtcaggtgaa cgctgctcca accgttgcat
     9841 aacaacaaag acgccttcat gttatactgc ggcaaaatac tgatgatgtg tcgcgattgc
     9901 ggccaaccgt ttccacccca ggcgagagac aatgtttgat aatttaaccg atcgtttgtc
     9961 gcgcacgctg cgcaatatca gtggccgtgg acgcctcact gaagacaacg taaaagatac
    10021 gctgcgcgaa gtgcgcatgg cgctgctgga ggcggacgtc gctctgccgg tagtgcgtga
    10081 gtttatcaat cgcgtaaaag agaaagcggt tggtcatgaa gttaataaga gcctgacgcc
    10141 ggggcaggag ttcgtcaaaa tcgtccgtaa cgaactggtt gcggcgatgg gcgaagagaa
    10201 ccagaccctg aacctggcgg cgcaaccgcc tgcggtcgtg ctgatggcgg gcctgcaagg
    10261 tgccggtaaa acaaccagcg ttggtaaact cggtaagttc ctgcgcgaga agcacaagaa
    10321 gaaagtgctg gtggtttctg ccgacgttta tcgcccggcg gcaatcaaac agcttgagac
    10381 gctggcagag caggtgggcg ttgatttctt cccttctgat gttggtcaga agccggtaga
    10441 tatcgttaac gcggcgctga aagaagccaa actgaaattc tacgacgtgc tgctggtgga
    10501 taccgctggt cgtctgcacg ttgacgaagc gatgatggac gagatcaaac aagtccatgc
    10561 gtcgattaac ccggttgaaa ccctgtttgt ggttgacgcc atgaccggtc aggatgcggc
    10621 caatacggca aaagcattca atgaagcgtt accgcttacc ggcgtagtgt tgaccaaagt
    10681 ggacggcgat gcccgcggcg gtgcggcgct ctctattcgt cacatcactg gcaaaccgat
    10741 caagtttctc ggtgttggcg agaaaactga ggcgctggag ccgttccatc cggaccgcat
    10801 cgcgtcgcgt attctcggca tgggcgacgt actgtcgctg atcgaagata tcgaaagcaa
    10861 agttgaccgc gcgcaggcag agaaattagc cagcaagctg aaaaaaggcg acggcttcga
    10921 tctcaacgat tttcttgagc aactgcgcca gatgaaaaat atgggcggca tggctagcct
    10981 gatgggcaag ctgccgggca tggggcagat cccggataat gttaagtcgc agatggacga
    11041 taaagtgctg gtgcgtatgg aagccatcat caactcgatg acgatgaaag agcgcgctaa
    11101 gccagaaatc atcaaaggtt cgcgtaagcg tcgtatcgct gccggttgcg gtatgcaggt
    11161 gcaggacgtg aaccgccttc tgaagcagtt cgacgacatg cagcgcatga tgaagaaaat
    11221 gaagaaaggc ggaatggcga agatgatgag aagcatgaag gggatgatgc cgccgggatt
    11281 tcctggtcgc taaatgacct tatgcgacag agttattaac tgctgattgc attttctcca
    11341 gaaatcagta aaattttcgg gcttttaata tgacaccgga ctccgttcct cgatggggtc
    11401 cggttgtttt attcacacaa gaggatgtta tggtaactat tcgtttagca cgtcacggcg
    11461 ctaaaaagcg tccgttctac caggttgttg tcgctgacag ccgtaatgca cgcaacggtc
    11521 gcttcatcga gcgcgttggt ttcttcaacc caatcgctag cgaaaaagaa gaaggcactc
    11581 gcctggatct ggatcgcatc gctcactggg ttggccaggg cgcaactatt tctgatcgcg
    11641 ttgctgcgct gatcaaagaa gtaaacaaag cagcttaatc tgtcacggtg gtcatgatga
    11701 gcaaacaact caccgcgcaa gcacctgttg atcccatcgt tttgggaaaa atgggttcgt
    11761 cttacggtat tcgtgggtgg ctcagagtgt tttcttccac cgaagacgcc gaaagcattt
    11821 ttgactatca gccctggttt atccagaagg cgggtcagtg gcagcaagtc cagctggaaa
    11881 gctggaagca ccacaatcag gacatgatca tcaagctgaa aggcgttgac gatcgtgatg
    11941 cggcgaacct gctgacgaat tgtgaaattg tcgtggattc atcgcagctg cctcagcttg
    12001 aagaggggga ctactactgg aaagacctga tgggctgcca ggtagtaacc actgaaggct
    12061 acgatctcgg taaagtcgtc gatatgatgg aaaccggatc taatgacgtt ctcgtcatta
    12121 aggcaaacct gaaagatgcg tttggtatca aggaacgtct cgtaccgttc ctcgatgggc
    12181 aggttatcaa gaaagtcgat ctcactactc gttcaatcga agtagattgg gatcctggtt
    12241 tttaaaccac cggataaacg gtaaaagacg gcgctatgtg gattggcata attagcctgt
    12301 ttcctgaaat gttccgcgca attaccgatt acggggtaac tggccgggca gttaaaaatg
    12361 gcctgctgag catccagagt tggagtcctc gcgacttcac gcatgaccgg caccgtaccg
    12421 tggacgatcg tccttacggc ggcggaccgg ggatgttaat gatggtgcaa cccttgcggg
    12481 acgccattca tgcagcaaaa gccgcggcgg gtgaaggcgc aaaggtgatt tatctgtcac
    12541 cacagggacg caagcttgat caagcgggcg tcagcgaact ggcaacgaat caaaaattga
    12601 ttctggtgtg cggtcgctac gaaggtatag atgagcgcgt gatccaaacc gaaattgacg
    12661 aagaatggtc aatcggcgat tacgttctca gtggtggtga gttaccagca atgacgctga
    12721 ttgactccgt ttcccggttt attccgggag tactgggaca tgaagcctcg gcaacggaag
    12781 attcctttgc tgaaggattg ctggattgcc cgcactatac gcggcctgag gtgttagaag
    12841 ggatggaagt tccgccagtg ttactgtcgg gcaaccatgc cgagatacgt cgctggcgtt
    12901 tgaaacagtc gctgggccgt acctggctta gaagacctga acttctggaa aacctggctc
    12961 tgactgaaga gcaagcaagg ttgctggcgg agttcaaaac ggaacacgca caacagcaac
    13021 ataaacatga tgggatggcg taagcccccg agatatcagt ttacccagga taagagatta
    13081 aattatgagc aacattatta agcaacttga acaagagcag atgaagcagg acgtaccttc
    13141 cttccgtccg ggtgataccg tggaagtgaa agtatgggtt gttgaaggtt ccaaaaaacg
    13201 tctgcaggca ttcgagggcg tggttatcgc tattcgtaac cgcggtctgc actctgcatt
    13261 cactgttcgt aaaatttcca acggcgaagg cgttgagcgt gtcttccaga ctcactctcc
    13321 ggtagttgac agcatttctg tcaaacgtcg tggtgctgtt cgtaaagcta aactgtacta
    13381 cctgcgtgag cgtactggta aggctgctcg tatcaaagag cgtcttaact aagatttcgc
    13441 ttaagcgaca tcctgttaag aagggctggc caattggctg gccctttttt tgtctgtttg
    13501 ctcgtgtttt aaggggttgt aatcaccact gcgacgcggc ggttctcggc gcggccctgg
    13561 gcggtcttgt tactggctat gggatatttt tttcctaaac cctgtgtggt gagattgctg
    13621 cgtggaattt gtccacccat agcccatgca tcggccacga cattcgcccg tttcaatgat
    13681 aagccttcgt tgtaactgtc ttcaccatag ttatcggtgt gtccatccat acgggcatgt
    13741 gttagccctg tcgaggccaa tttggctgcc atggtttgga tctgttgctg gctttccggg
    13801 agcaatttgt agtcattttt tgcgaacaga atggcatctg ataagcccag cgaccagtcg
    13861 ccggcggatt cagtaaatcc ataagattgc atagcggcga cttgctcagg agtaaacttt
    13921 ccctgagggg actggcagcc agtcagtatt agtgaggtga aaatcagggg tgctaccagg
    13981 tgctttatca tatcgatata tccttgttat ctcaccagct tttcggcacg ctggtgtttg
    14041 gcctgataca tattgtgatc ggcaagctct tgtaattttt ccgcagaggc gtgctcaatg
    14101 gtcatcgcgt aaccaatgct taatgtcatg gtggtctgat ggccattatg aagatcaaac
    14161 gggagattaa agatttgtgt cagtgctgag catatctgct gcacttcaga ttctgactgt
    14221 acatcataga gcaccatagc gaattcatcg ccgcccaggc ggtatgcttt atgtcgcagc
    14281 ccgccaactt cagctaaccg ttttgcgatt tcaatcaaga ctctatcgcc cgtcgcatga
    14341 ccccaggtat cattgatgta tttgaaatta tcgccatcaa gaaatagtaa cgccgacgtt
    14401 tttcgggcat cggaattgtt catcaacgtg ttgatgccgc tacgaaacgc tgcgcggtta
    14461 gccagcccgg ttaatgggtc atgtagcgcg gtacgtagaa gctgcgcatt tttagcctgt
    14521 aaacgaagct gccactcttc catttcatcc agcagactgt tgaagtcgag agcgaagcgg
    14581 tgaaactcag cgatacgttc ttccgaaact cgtcgggaaa aattgcggtt ggaacgcaca
    14641 tcatgtacga catcggtgat atttttcagt gcttccacca ggccattgtg caaatggcgg
    14701 gtgagggtaa ttgcgattcc tgatgccagc agaatacaac cggtcagtac ggcgagcgaa
    14761 aaccagataa aatggctgat tgaactgtcg cgagcggtta agcgtacttc gccaatggtt
    14821 tcgccattgt gacgaatcgg ctgaatgatg ggggcaggga agagccagtg gcttatgaaa
    14881 ttgctgaaag tatcgcctgg atccttacgg gtgtaatgcc aggatgccag aatattttgc
    14941 tgcttatcac gtacttctgc agttgaaaat tgcccttgct ggcccagcgc tgccagtgtt
    15001 tcagttgctg cagggccatc ggcaaaaacg acagctgctt ccagactgta agtcattgtt
    15061 gctgctgtca gtgccaggtt tttttgcgcg tactgtttca gggtcagcac cgaggtcacg
    15121 gaaagcagca accagatcag catcatagtg ataaatatgc tggtcatact gatgttgcgt
    15181 aatgctcttt taaacgtggg gcgcttatta agagaattat cgttatccat catttttctt
    15241 ccgtgcgagc attaagacat ccgggttgac ctttacaccg ctacgcgata aggcatccag
    15301 gtttacggca aatctgacgt cattattgtg gatgatcaga caaaaggcgc taccaataat
    15361 gcactcggta ttttgttcgg caattaataa taacgccttt gacggatatt gttcggttaa
    15421 ttccatctgg aatgtcggtg actcgttgcc aaaataaaaa ccattgcagc ctgaaatcat
    15481 cgcctcttgt tgagtgtgaa tgatgacagg cagataaggc aaagatgtcg cggcattctc
    15541 ttgtagggcg gtactaaaac gtgaagatga aaagatacat aactttggtg gccctgatag
    15601 cgcaggccag cgggtgtaac tgacaatacc ggaaaccatc atacgcacgt ttttcgcaac
    15661 gtcagaaggc tcctgagcga gtattggtgc tcccgtcagc aggagaatca gaagtaagaa
    15721 cagtcggtga gaaaaacgca ttcagggatc cccaccatct tcgctcgaaa tgcatcgata
    15781 ttttttcaga atgctcatta aaatatcatt ttttcatgcg atcgtcatca atgtga
//
LOCUS       DAPGWD010000094        15774 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPGWD010000094 DAPGWD010000000
VERSION     DAPGWD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.095"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..880
                     /locus_tag="RHX88_002928"
     CDS             <1..880
                     /locus_tag="RHX88_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2231596.1"
                     /translation="HITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQL
                     GVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYI
                     LPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLED
                     ELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELG
                     NSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSN
                     ALRRFLDYCDPANVPR"
     gene            1085..2554
                     /gene="lysP"
                     /locus_tag="RHX88_002929"
     CDS             1085..2554
                     /gene="lysP"
                     /locus_tag="RHX88_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDV2231597.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2847..4838
                     /gene="cirA"
                     /locus_tag="RHX88_002930"
     CDS             2847..4838
                     /gene="cirA"
                     /locus_tag="RHX88_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDV2231598.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4870..5706)
                     /gene="yeiG"
                     /locus_tag="RHX88_002931"
     CDS             complement(4870..5706)
                     /gene="yeiG"
                     /locus_tag="RHX88_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV2231599.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5964..6632
                     /gene="folE"
                     /locus_tag="RHX88_002932"
     CDS             5964..6632
                     /gene="folE"
                     /locus_tag="RHX88_002932"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDV2231600.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6649..7806
                     /locus_tag="RHX88_002933"
     CDS             6649..7806
                     /locus_tag="RHX88_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDV2231601.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7948..8988
                     /gene="galS"
                     /locus_tag="RHX88_002934"
     CDS             7948..8988
                     /gene="galS"
                     /locus_tag="RHX88_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDV2231602.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            9268..10266
                     /gene="mglB"
                     /locus_tag="RHX88_002935"
     CDS             9268..10266
                     /gene="mglB"
                     /locus_tag="RHX88_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDV2231603.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10327..11847
                     /gene="mglA"
                     /locus_tag="RHX88_002936"
     CDS             10327..11847
                     /gene="mglA"
                     /locus_tag="RHX88_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDV2231604.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11863..12873
                     /gene="mglC"
                     /locus_tag="RHX88_002937"
     CDS             11863..12873
                     /gene="mglC"
                     /locus_tag="RHX88_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDV2231605.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12944..14179)
                     /gene="preA"
                     /locus_tag="RHX88_002938"
     CDS             complement(12944..14179)
                     /gene="preA"
                     /locus_tag="RHX88_002938"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDV2231606.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELCDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14173..15411)
                     /gene="preT"
                     /locus_tag="RHX88_002939"
     CDS             complement(14173..15411)
                     /gene="preT"
                     /locus_tag="RHX88_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDV2231607.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         3774 a         3880 c         4118 g         4002 t
ORIGIN      
        1 gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg gatcaaccac
       61 ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct tgaccgacct
      121 ggaagggcag cttggcgtgc aactgtttga tcgcgtgggg aaaagactgg ttgttaatga
      181 acacgggcgg ctgctctatc cgcgtgcgtt ggccttgctt gaacaggcgg ttgaaatcga
      241 acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta ccatcggtaa
      301 ctacattctg cctgcagtta tcgcccgtta tcgccatgat tatccgcagt taccgattga
      361 acttagcgtt gggaacagcc aggacgtgat gcaagcggtg ctggatttcc gcgttgatat
      421 tggttttatt gaaggaccgt gccacagcac tgaaattatt tctgaaccgt ggctggaaga
      481 cgagctggtg gttttcgccg cgccgacttc gccgttggcc cgtggtccgg tcaccttaga
      541 gcagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc gggagattgt
      601 cgattatcta ttgctgtcac atttaccgaa gtttgagatg gcgatggaat taggtaactc
      661 cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt cgcgacgggt
      721 gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc tgccgcgcct
      781 gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg cgctacggcg
      841 ctttctggac tattgcgatc ccgcgaatgt accgcgttaa gttgctgtac aagaacatgc
      901 tggtgctgtg tcgatttcgt gacgcagcgc cctcagcatg cattcgccag aaaagagatt
      961 ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg tgtatttctg
     1021 ccggaaggat tgccaatcgt ctgctacaat cgcgcctcat ttttaagatg gatagcattt
     1081 ttgtatggtt tccgaaacta aaaccacaga agcgccgggc ttacgccgtg aattaaaggc
     1141 gcgtcacctg acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc
     1201 tggcgcaacg atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg
     1261 cctgatggtt tacttcctga tgaccagtct cggtgaactg gctgcatata tgccggtttc
     1321 cggttcgttt gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg
     1381 ctggaactac tggtacaact gggcggtgac tatcgccgtt gacctggttg cagctcagct
     1441 ggtcatgagc tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg
     1501 cgttatcttc ctgctgaact acatctcagt tcgtggcttt ggtgaagcgg aatactggtt
     1561 ctcactgatc aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat
     1621 cggtatcttc aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc
     1681 gtttgctggt ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca
     1741 gggaaccgag ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc
     1801 acgcgcggta cgtcaggtgt tctggcgaat cctgttgttc tatgtgttcg cgatcctgat
     1861 tatcagcctg attattccgt acaccgatcc gagcctgctg cgtaacgatg ttaaagacat
     1921 cagcgttagt ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg cggcggcggt
     1981 gatgaacgca gttattctga cggcggtgct gtcagcgggt aactccggta tgtatgcgtc
     2041 tactcgtatg ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaact
     2101 gtcgcgtggt ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtctgtg
     2161 cttcctgacc tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg
     2221 gatgacgggt tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg
     2281 ttacgtattg cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact
     2341 ggggccgatc ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc
     2401 gttcctgaaa gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct
     2461 gttcctgatt atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag
     2521 cgaaatgaag ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct
     2581 ctcaaccgag agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt
     2641 aacatttgga ttgataattg ttatcgtttg cattatcgtt acgccgcaat caaaaaaggc
     2701 tgacaaatca gaggctgttc cggctttctg ggatgatcac ctgcatacaa ataagtccac
     2761 cgcgatgctg ccgtacgcaa ggggacgtga agaagatgtg agcgataacc cattttattt
     2821 tcgtagttac ctcatggaga tatggaatgt ttaggttgaa ccctttcgta cgggtcgggc
     2881 tgtgtttgtc cgctatttct tgtgcatggc ctgtgttagc ggtcgatgat gatggcgaaa
     2941 cgatggttgt cactgcatct tccgttgaac aaaacctcaa agatgcaccc gccagtatca
     3001 gcgtcattac ccaggaagac ctgcagcgaa aaccggtaca gaatctgaag gatgtcctca
     3061 aagaagtgcc tggcgtacaa ctgacgaacg aaggggataa ccgtaagggc gttagtattc
     3121 gtggtctgga cagcagctac accctgattc tcgtcgacgg taaacgcgtt aactcccgca
     3181 atgccgtctt ccgccacaat gatttcgatc tgaactggat cccggtcgat tccatcgaac
     3241 gtattgaagt ggttcgtggc ccgatgtcgt cgctatacgg ctccgatgcg ctcggcggtg
     3301 tagtgaatat catcaccaaa aaaatcggtc agaaatggtc gggtaccgtt accgtcgata
     3361 ccaccattca ggaacatcgc gatcgcggtg atacctataa cggtcagttc tttaccagtg
     3421 gaccattaat tgatggtgtg ctgggaatga aagcttacgg cagcctggca aaacgtgaaa
     3481 aggatgaccc gcaaaactca acgaccaccg ataccggaga aacgccgcgt attgaaggat
     3541 tctccagccg cgacggcaat gtcgaatttg cctggacacc gaatcaaaat cacgatttta
     3601 ctgccggata cggtttcgac cgtcaggatc gtgattccga ctcgctggac aaaaaccgcc
     3661 tggaacgcca gaactactcc gtcagccata atgggcgttg ggattacggc accagcgaac
     3721 tgaaatacta cggtgagaaa gtcgagaaca aaaaccctgg caacagcagc ccgataactt
     3781 ccgaaagcaa tacggtcgac ggcaaataca cgttgccgct gacggcgatt aatcagtttc
     3841 tcacggttgg cggtgaatgg cgtcacgaca aacttagcga tgcggtgaac ctgaccgggg
     3901 gaaccagctc caaaacgtct gccagccagt acgcgctatt tgtggaagat gaatggcgga
     3961 tcttcgagcc gctggcgctg acgaccggcg tgcgtatgga cgatcacgaa acctacggtg
     4021 aacactggag tccgcgtgcc tacctggttt ataacgccac cgacaccgta acggtgaaag
     4081 ggggctgggc gacggcattt aaagcacctt ctctgttgca acttagccct gactggacga
     4141 gcaattcctg ccgtggcgca tgtaagattg tgggtagccc ggatctgaaa ccagaaacca
     4201 gcgaaagttg ggagctgggg ctttactaca tgggtgaaga aggctggctg gaaggggttg
     4261 aatccagcgt taccgttttc cgtaacgatg tgaaagatcg catcagcatc agccgtacgt
     4321 ctgacgtcaa tgctgcaccg ggctaccaaa actttgtcgg ttttgagacg ggcgctaacg
     4381 gacggcgcat accggtattt agctactaca acgttaacaa agctcgtatt cagggcgtgg
     4441 aaaccgaact gaaaattccg ttcaacgatg aatggaaact gtcactcaac tacacctaca
     4501 acgatggtcg tgatgtcagc aacggcgaaa acaaaccgct atccgatctg ccgttccata
     4561 ctgctaacgg tacgctggac tggaaaccgc tggcgctgga agactggtca ttctatgttt
     4621 ctggtcacta taccgggcag aaacgcgccg acagcgcgac ggctaaaaca ccgggcggtt
     4681 ataccatctg gaataccggc gcggcctggc aggtgactaa agacgtcaaa ctgcgcgcag
     4741 gcgtgctgaa ccttggcgac aaggatctca gtcgtgacga ctacagctat aacgaagacg
     4801 gacgtcgtta ctttatggca gtggattatc gcttctgatg agaagatgcc cggcgaaccg
     4861 ggcggacttt cacttcagta aatactgcgc atggaagcgc aggtgatcct ctataaaaga
     4921 ggcgatgaag tagtaactgt gatcatatcc cggctgaata cgcagcgtca tcggccacgc
     4981 tttctggcgc gcggcttctg ccagtaccgc aggttgcaac tggtcggcaa gaaactgatc
     5041 attatcgccc tgatcgataa gcgtcgggat cgcatcctgc gcgttactgg catacatcag
     5101 cgcgcaactg tcccattcca gccatgcatt tttgtcctca cctaaatagc tgctaaacgc
     5161 tttgattccc cacgggacgc tgcacggatt cacaattggc gcaaaggccg aaacgctggt
     5221 gtatttaccc ggatttttca gcgccataat cagcgcaccg tgaccaccca ttgaatgacc
     5281 gctaatggcg cagcggtcgc tgacattaaa ttgcgactga accagcgccg gtaattcatc
     5341 gcgcagataa tcatacatcc ggtaatgcgt cgcccacggc ggttgcgtgg cgttaagata
     5401 aaaccccgcg ccctggccta aatcgtagcc atcatcgttg gcaacctgtt cgccgcgcgg
     5461 gctggtgtct ggcatcacca gtacaatccc cagttctgcc gccacgcgct gggcacccgc
     5521 cttgatggtg aagttctcgt cattgcaggt taatccggaa agccagtaca gcactggtgg
     5581 cggggtgtga tcacgaggtg gtgggagaaa gatactgaac gtcatcggac agtttaaggt
     5641 actggagtcg tgtcgccagc gttgctgcca gccttcaaaa cagcggtgct cttcgagcat
     5701 ttccatgcgt ggctccttgt tgtgttgttt gcaatttctt catcataccg tttattcaat
     5761 gtgctgtgag taactttcac ttccgtattt gcataacgat gttttaacat ctgctgatga
     5821 aaggcagcgg caattacaat aattatcgct gtgaatactg gattatgtgc gccgcctcac
     5881 gcacaataat caagctgtaa atcagcttaa taactttgcc cccacgcagg gcggaggcgt
     5941 cacacctgca ggagaaatca taaatgccat cactcagtaa agaagcggcc ctggttcatg
     6001 aagcgttagt tgcgcgagga ctggaaacac cgctgcgccc gcccgtgcat gaaatggata
     6061 acgaaacgcg caaaagcctt attgctggtc atatgaccga aatcatgcag ctgctgaatc
     6121 tcgacctggc tgatgacagt ttgatggaaa cgccgcatcg catcgctaaa atgtatgtcg
     6181 atgaaatttt ctccggtctg gattacgcca acttcccgaa aatcaccctc attgaaaaca
     6241 aaatgaaggt cgatgaaatg gtcaccgtgc gcgatatcac tctgaccagt acctgtgaac
     6301 accattttgt taccatcgat ggcaaagcga cggtggctta tatcccgaaa gattcggtga
     6361 tcggtctgtc aaaaattaac cgtatcgtgc agttctttgc ccagcgtccg caggtgcagg
     6421 aacgtctgac gcagcaaatt cttattgcgc tacaaacgct gctgggcacc aataacgtgg
     6481 ctgtctcgat cgacgcggtg cattactgcg tgaaggcgcg tggcatccgc gatgcaacca
     6541 gtgccacaac aacgacctct cttggtggat tgttcaaatc cagtcagaat acgcgccacg
     6601 agtttctgcg cgctgtgcgt catcacaact gattaaaagg caggaaccat ggagcgcaac
     6661 gtcacgctcg attttgttcg cggcgtcgcc attctgggga tcctgctatt aaacatcagc
     6721 gcctttgggc taccaaaggc ggcttatctc aatcccgcct ggtacggtgc tattacgccg
     6781 caggatgcat ggacctgggc atttcttgat ctcatcgccc aggtgaaatt cctcacgctt
     6841 tttgcgctgc tgtttggtgc tggactgcaa atgttgctgc cccgtggcag acgctggatc
     6901 cagtcgcggt taacgctgtt agtcttattg ggctttattc acggtttatt gttctgggac
     6961 ggcgatattc tgctggctta cgggctggtg ggcttaatct gctggcggct ggtgcgcgat
     7021 gcgccatcgg taaaaagcct gtttaataca ggcgtcatgc tttatctggt ggggcttggc
     7081 gttttgctgt tattggggct gatttccgac agccagacca gccgcgcctg gacgccggat
     7141 gcatcggcta ttttgtatga aaaatactgg aagcttcacg gcggcgttga agcgatcagt
     7201 aatcgtgccg atggtgttgg caacagttta ctggcactgg gcgcacagta tggctggcaa
     7261 ctggcaggga tgatgctcat tggtgcggcg ctgatgcgca gcggctggct gaaagggcag
     7321 ttcagcttac gtcactatcg tcgtactggt tttgtgctgg tggcgattgg ggtgaccatt
     7381 aaccttcctg ccatcgccct gcaatggcag ctggactggg cgtatcgctg gtgtgccttc
     7441 ttacttcaaa tgccgcggga actgagtgcg ccgtttcagg cgattggcta tgcgtcgctg
     7501 ttttatggct tctggccgca attgagccgc tttaagctgg tgcttgcgat cgcctgcgtc
     7561 ggacggatgg cgctgaccaa ctatctattg caaacgctga tttgtaccat gcttttttac
     7621 cacctcggtt tgtttatgca ttttgatcgc ctggagctgc tggcgtttgt tattccggta
     7681 tggctggcga atatcctctt ctctgttatc tggctgcgtt acttccgcca ggggccggtg
     7741 gaatggctct ggcgtcagtt aactttgcgt gctgccggaa cggcaatatc taaaacatca
     7801 agataacgat ctggatcaca ttcgttaaca aaacggctgt aaccgtttcc attgctgtga
     7861 ctcgattcac gaagtcctgt attcagtgct gacaaaatag ccgccagcaa gcagtcattt
     7921 actgcaatct cataacaggt agtgaatatg atcaccattc gtgatgtagc gcgtcaggct
     7981 ggcgtctctg tggcaacggt ttcccgggtg ctcaataaca gcacgctggt cagtgccgac
     8041 acgcgtgaag cagtaatgaa agccgtgagt gagctggatt atcggccaaa tgccaatgcc
     8101 caggcgctgg caactcaggt tagcgatacc attggcgtgg tggtgatgga cgtttctgat
     8161 gcttttttcg gcgcgctggt aaaagcggtg gatctggtcg cccagcagca tcagaaatac
     8221 gtgctaatcg gcaatagcta tcatgaagcg gaaaaagagc gtcacgccat tgaggtgtta
     8281 attcgccagc gttgtaatgc gttgattgtt cactcaaaag cattgagtga cgatgaactg
     8341 gcgcaattta tggataacat tcccggtatg gtgttaatca accgcgttgt gccggggtac
     8401 gcccatcgtt gcgtttgcct ggataatctc agcggtgccc gaatggcgac gcgcatgttg
     8461 ctgaataacg gtcatcaacg tattggttat ctttcttcca gccacggcat tgaagatgac
     8521 gccatgcgta aagcaggctg gatgagtgcg ttgaaagagc aggatattat tccgccggaa
     8581 agctggattg gcactggtac gccggacatg ccgggcggtg aggcggcgat ggttgaactg
     8641 ctggggcgca atctacaact taccgctgta tttgcttata acgacaatat ggccgctggc
     8701 gcactgacag cattaaaaga taatggcatt gcgattccgt tacatctctc aatcatcggt
     8761 ttcgatgata ttcccatcgc ccgttacacc gacccgcaat taacgaccgt gcgttatccc
     8821 attgcttcaa tggcgaaatt agccaccgaa ctggccttgc agggggcagc aggcaatatt
     8881 gatcctcgtg ccagccactg ttttatgccg acgttagtgc gtcgccattc tgtcgcaaca
     8941 cgccagaatg cggcgacgat cactaactca acaaatcagg cgatgtaacc gctttcaatc
     9001 tgtgagtgat ttcacagtat cttaacaatg tgatagctat gattgcaccg ttttaacgtt
     9061 gtaacccgta tgtaacagtg aataatcact ttcaccgagg taatagcgtc ataacaacaa
     9121 ttaaagccgt tttctggagc gttaccgggc atggaagaac gaattttaaa aagtgagctt
     9181 cggcgttcag taacacttca ttaactctac tgccccgccg agcatttatc tcaagcacta
     9241 ccctgcataa gaaaaaccgg agataccatg aataagaagg tgttaaccct gtctgctgtg
     9301 atggccagca tgttattcgg tgccgctgca cacgctgctg atactcgcat tggtgtaaca
     9361 atctataagt acgacgataa ctttatgtct gtagtgcgca aggctattga gcaagatgcg
     9421 aaagccgcgc cagatgttca gctgctgatg aatgattctc agaatgacca gtccaagcag
     9481 aacgatcaga tcgacgtatt gctggcgaaa ggggtgaagg cactggcaat caacctggtt
     9541 gacccggcag ctgcgggtac ggtgattgag aaagcgcgtg ggcaaaacgt gccggtggtt
     9601 ttcttcaaca aagaaccgtc tcgtaaggcg ctggatagct acgacaaagc ctactacgtt
     9661 ggcactgatt ccaaagagtc cggcattatt cagggcgatt tgattgctaa acactgggcg
     9721 gcgaatcagg gttgggatct gaacaaagac ggtcagattc aattcgtatt gctgaaaggt
     9781 gaaccgggcc atccggatgc agaagcacgt accacttacg tgattaaaga gctgaacgac
     9841 aaaggcatta aaactgaaca gttacagtta gataccgcta tgtgggatac cgctcaggcg
     9901 aaagataaga tggacgcctg gctgtctggc ccgaacgcca acaaaatcga agtggttatc
     9961 gccaacaacg atgcgatggc aatgggcgcg gtagaagcac tgaaagcaca caacaagtcc
    10021 agcattccgg tgtttggcgt cgatgcgctg ccagaagcgc tggcgctggt gaaatccggt
    10081 gcactggcgg gcacagtact gaacgatgct aacaaccagg cgaaagcgac ctttgatctg
    10141 gcgaaaaacc tggccgatgg caaaggtgcg gctgatggca ccaactggaa aatcgacaac
    10201 aaagtggtcc gcgtacctta tgttggcgta gataaagaca acctggctga attcagcaag
    10261 aaataagact gattgggcgc agtcgtttac tgcgccctta ttgtatggcc aacaaggtaa
    10321 aattatatgg tcagctcaac gactccgtct tccggggaat acttgttgga aatgagcggt
    10381 atcaacaagt cctttcctgg tgttaaggca cttgataacg ttaatttaaa agtccggcca
    10441 cattctatcc atgcattaat gggggaaaac ggtgcaggaa aatcgacatt attaaaatgc
    10501 ctgtttggta tttatcaaaa agactccggc accattttat tccagggtaa agagatcgat
    10561 ttccattctg ccaaagaagc actggaaaat ggtatttcga tggtacacca ggagttaaac
    10621 ctggtattac aacgttcggt gatggataac atgtggctgg ggcgatatcc caccaaaggc
    10681 atgtttgtcg atcaggacaa aatgtaccgc gaaaccaaag cgatttttga tgaactggat
    10741 attgatatcg atccgcgtgc gcgcgtcggc acattatccg tttcgcaaat gcagatgatc
    10801 gaaatcgcca aagcgttttc ctataacgcg aaaattgtga ttatggatga accgacttct
    10861 tcgttaaccg aaaaagaggt caatcatctg ttcactatta ttcgtaaatt aaaagagcgc
    10921 ggctgcggta ttgtttatat ctcgcataaa atggaagaaa tcttccagtt atgtgatgaa
    10981 gttaccgtat tgcgcgacgg tcagtggatc gccaccgaac cgctggcagg actgacgatg
    11041 gacaagatca tcgccatgat ggttgggcgt tctcttaatc agcgttttcc tgacaaagaa
    11101 aacaagccgg gcgaagtcat cctcgaggtg cgtaacctaa cgtcactgcg ccagccgtcg
    11161 attcgcgatg tctcgtttga tctgcataaa ggggagatcc tcggtattgc cgggctggtg
    11221 ggggcgaaac gtaccgatat tgttgagacg ttatttggta ttcgcgagaa atcggctggc
    11281 accattacgt tgcacggcaa acagatcaat aaccataatg ccaacgaagc cataaaccac
    11341 ggatttgcac tggtaactga ggagcgccgc tcaacgggaa tttatgccta tctggatatt
    11401 ggttttaact cgttaatttc caatattcgc aactacaaaa ataaagttgg tttactggat
    11461 aactcgcgga tgaaaagcga tacccagtgg gtgattgatt cgatgcgggt aaaaacgccg
    11521 ggtcatcgga cgcaaattgg ttcgctctcc ggtggtaatc agcaaaaggt gattattggt
    11581 cgctggctac taacgcaacc agaaatatta atgctcgatg aaccgacgcg cggtattgat
    11641 gtcggggcga agtttgaaat ttatcagtta attgccgaac tggcgaagaa aggcaagggg
    11701 attattatta tctcctctga aatgcctgag ttgttaggga taacagaccg tattctggtc
    11761 atgagcaatg gtctcgtttc cggaattgtc gatacaaaaa caacaacgca aaacgaaatt
    11821 ctgcgtcttg cgtctttgca cctttaagat taggggcttc ccatgagtgc gttaaataag
    11881 aaaagttttc ttacttacct gaaagagggc ggtatttacg tcgttctttt agttttgctg
    11941 gcgattatta ttttccagga cccaacattt ttaagtctgt tgaacttaag taatattctc
    12001 acccagtcat cggtgcgtat tattatcgcg ctcggtgtgg cagggttaat tgtcacccag
    12061 gggaccgatc tttctgctgg tcgtcaggta gggctggcgg cagtggtggc tgcgacatta
    12121 ttgcagtcca tggataacgc caacaaagtg ttcccggaaa tggcgacgat gccgattgcg
    12181 ctggttattc tgattgtctg tgccattggt gcggtgatcg gtttgatcaa cggtctgatt
    12241 atcgcttatc tcaacgtgac gccgttcatt accacgctcg gcacgatgat catcgtctat
    12301 ggcatcaact cgctctatta cgactttgtc ggggcgtcgc caatttctgg ttttgacagt
    12361 ggcttctcta cctttgctca gggctttgtc gcgctgggga gtttccgtct ctcttacatc
    12421 accttctacg cgttgattgc ggtggcgttc gtctgggtgt tgtggaacaa aacccgcttc
    12481 ggtaagaaca tttttgccat tggcggtaac ccggaagcgg caaaagtatc tggtgtcaac
    12541 gtcggcctga acctgctgat gatctacgcg ttgtctggcg tgttctatgc ctttggcggg
    12601 atgttagaag ccggacgtat cggctctgcc accaacaacc tcggctttat gtatgagctg
    12661 gatgctatcg cggcgtgcgt ggtaggcggt gtatcgttca gcggcggtgt ggggacggtg
    12721 attggcgtgg tgaccggggt aattattttt accgtcatca actatggcct gacgtatatc
    12781 ggcgtaaacc catactggca gtacatcatc aaaggggcga ttattatctt cgccgtagcg
    12841 ctggattcac tgaaatacgc gcgtaagaag tgatgtgatt gtggatattg ccggatgcgc
    12901 tgcatccggc aatcggtgcc aaatgccgga tgcgtctctg gttttacaac gttaccgggt
    12961 gttctttctc gcctttctta aacttcactt ccccgagctc aatacaaccc accgggcaga
    13021 cgtgaccaca aagcagacaa cccacacatt tctcggtatt acaatgcggt gtgcgggttt
    13081 tctcgctcca ttccatcgcc tggtgaccgc cgtcgtaaca ggaaatataa cagcgtccac
    13141 agccaacaca tttatcaaga ttgatacggg gatagacaat ataactgcgg tctaaatctt
    13201 ccgccgggac aatattgtta ttcgccagac ctaccatctc ctgcagcgaa tcaaatccct
    13261 gatcggcgag ataatgcgac aacccgcttg ccatatcttc cactatccga tacccgtact
    13321 gcatgatgcc ggtggtcacc tgtaacgttg ctgcgccgag caataaaaac tcagccgcat
    13381 cctcccaggt ttcaatgccg ccgataccgc tgattgggaa atcgcacagt tctggatggg
    13441 tgcgcatctg ctgaatgaag cgcagggcga tcggttttac cgctttaccg gaatatccgg
    13501 aaatactcga ttttccgtta acgatcggca taccgatttt ctgattaaga tcgatattgg
    13561 tgatggattt aacggtgtta atcgccgcaa tgccatctgc gccgccgcgc ttcgccgcca
    13621 gcgccacttc gcacatatca ccgatattcg gcgtcatctt cgccagcatt ggcagcgtgg
    13681 aaccccgttt cactgcccga caatattttt ctaccagctc cgggctttgc ccgacatcgc
    13741 tacccatcgc atgagaagtc atttgcggac aggagaagtt acactcgatc atatccgcgc
    13801 cagcttcttg caccaggcgc gccagctcct cccattgctg ctcattttcc cccatgatcg
    13861 aagcgatcaa tactttgtcc gggtaatctt ccttcagctg acgcagggcg gccagattct
    13921 cttccaacgg atgttcagca atctgctcca tatttttgaa gccgataaaa ccggtatctt
    13981 ctttcaccag atgatcaaaa cgcggcgaga cttcgttggc gataaaaaag ccgatcgttt
    14041 taaacaccac gccgccccaa cctgtgtcgt aggctttggc acacatctca tagcagttgc
    14101 ctacaggcga agaagagagg cagaacgggt tgggaaactt cacgccgcaa aaagtaatcg
    14161 aaagatcttt cgttaacatg agcaagctcc ctctaaatag tgatgaatcg cctcggcggc
    14221 ttctttcccg gttttcacgg catagaccac ggttttgtca ccctcaacaa tatcgccagc
    14281 agcaaagact tgcggatcgc gggtctgata attttgtgtt ttgatggtgt tacgctgcgg
    14341 ctctaactcc gcaaaggcat ccagtctggc atgctgaccg acggcgagaa taattttatc
    14401 tgccgccatc gtcagttcgc ccggtaaccg tacatgctta aacgtgactt tattgccttc
    14461 cacggctact ggcgtgaatc catcgatgat cgaaacgccc agctcccggg cgctggcaaa
    14521 ctctttttcg cttgccggaa actcatctaa ctcttcacgc gctacgcagg ttaccgcctg
    14581 acagccgaga actttcagcg tgctggctac gtccatcgcg acatcaccgc cgccgataat
    14641 taatgcgctt tgtggaatgc tgatatcgcc ttgcgcctgt cgtacacgtt gcaggaagtc
    14701 gacggcaatc tcaacgtcac tatgctcaaa cagcggtagc ccggaaccgc ttgataaccc
    14761 cacggtgacc agtaccgcgc ggttttctgc tttcagctgc tcaagggtga gtgtgttacc
    14821 gacttcgttg ttgcacttaa tggtcacgcc cattttttca atacgggcga tctctgcatc
    14881 cagcactgat tgcggtaacc ggaattgcgg aataccgtta cgcaaccagc caccggggtg
    14941 cgcttctttc tcataaatcg tgacgtcata accttggttt gtcagtgtta cactggcctg
    15001 caatccggca ggacctgcgc caataatcgc gactttgccg agcgttttag taccgggctg
    15061 ataaatttcc atcccagttt gttgttcaaa atcagtaaca aaacgctgta agcgaccgat
    15121 atcaatggga gtatcaacac cggcacgggt acaaccgctt tgacataatt tctccgtcgg
    15181 gcacactctg gcacaaacgg caccgagggc attattttcg cgaattgtct cggcagcgcc
    15241 tttaaaatta cgaaagtaga ttgagcgaat aaatttcccc ggatcggtct gtgccgggca
    15301 agcctgacta cagggagcgt catgacataa taaacagcga gaggcttctt taatcgccag
    15361 taaaggcgta aaagccggag tgagttcatc cagataattt tgttgcggca tgtgatcttc
    15421 cttaatgcta tgagtaatta aggcgaacgc taaatatact cgtcatactt caagttgcat
    15481 gtgctgcgtc tgcgttcgct caccccagtc acttacttat gtaagctcct ggggactcac
    15541 tcacttgtcg ccttcctgca actcgaatta tttagagtat aaatattgcg tgctgatatt
    15601 aaggctatgc gcgtggggaa atttatactg tgaaaggatt cacaaatagc tgcggttcag
    15661 aatgttatat ccgcagctta ttgagtgaag taataattat tctcaataat taacagaaaa
    15721 gagaaaatat taacgataga aaataagata aacaatcatg ccgatagata aaat
//
LOCUS       DAPGWD010000095        15646 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPGWD010000095 DAPGWD010000000
VERSION     DAPGWD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.096"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..623
                     /locus_tag="RHX88_002940"
     CDS             81..623
                     /locus_tag="RHX88_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231608.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            723..1925
                     /locus_tag="RHX88_002941"
     CDS             723..1925
                     /locus_tag="RHX88_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDV2231609.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            1980..2450
                     /locus_tag="RHX88_002942"
     CDS             1980..2450
                     /locus_tag="RHX88_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV2231610.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIQRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(2491..3393)
                     /locus_tag="RHX88_002943"
     CDS             complement(2491..3393)
                     /locus_tag="RHX88_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDV2231611.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3587..4777)
                     /gene="glpC"
                     /locus_tag="RHX88_002944"
     CDS             complement(3587..4777)
                     /gene="glpC"
                     /locus_tag="RHX88_002944"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDV2231612.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4774..6033)
                     /gene="glpB"
                     /locus_tag="RHX88_002945"
     CDS             complement(4774..6033)
                     /gene="glpB"
                     /locus_tag="RHX88_002945"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDV2231613.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADNKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6023..7651)
                     /gene="glpA"
                     /locus_tag="RHX88_002946"
     CDS             complement(6023..7651)
                     /gene="glpA"
                     /locus_tag="RHX88_002946"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDV2231614.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7924..9282
                     /gene="glpT"
                     /locus_tag="RHX88_002947"
     CDS             7924..9282
                     /gene="glpT"
                     /locus_tag="RHX88_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDV2231615.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9287..10363
                     /gene="glpQ"
                     /locus_tag="RHX88_002948"
     CDS             9287..10363
                     /gene="glpQ"
                     /locus_tag="RHX88_002948"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV2231616.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10405..10644)
                     /locus_tag="RHX88_002949"
                     /pseudo
     CDS             complement(10405..10644)
                     /locus_tag="RHX88_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10826..11476
                     /gene="inaA"
                     /locus_tag="RHX88_002950"
     CDS             10826..11476
                     /gene="inaA"
                     /locus_tag="RHX88_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDV2231617.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11530..11784)
                     /gene="yfaE"
                     /locus_tag="RHX88_002951"
     CDS             complement(11530..11784)
                     /gene="yfaE"
                     /locus_tag="RHX88_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDV2231618.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(11784..12914)
                     /gene="nrdB"
                     /locus_tag="RHX88_002952"
     CDS             complement(11784..12914)
                     /gene="nrdB"
                     /locus_tag="RHX88_002952"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDV2231619.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(13003..15288)
                     /gene="nrdA"
                     /locus_tag="RHX88_002953"
     CDS             complement(13003..15288)
                     /gene="nrdA"
                     /locus_tag="RHX88_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDV2231620.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         3637 a         3871 c         4272 g         3866 t
ORIGIN      
        1 gctcattgaa tcctctgtgc caggcagtac aatcccgcag ctttgggaat tttgattaat
       61 tgcttgtggg aaaggggata atgaaaaaaa ttgcgctagc aggtctggcc ggaatgctgt
      121 tggtttctgc atcggtcaat gcaatgagca tcagcggcca ggcgggtaaa gaatacacca
      181 atattggtgt cggttttggg actgaatcga cgggcctggc tttaagcggt aactggacac
      241 ataacgacga cgacggtgac gtcgcgggcg tggggctggg gttgaatctg cctctcgggc
      301 cgttaatggc gaccgttggc ggaaaaggcg tttacaccaa cccgaattac ggcgatgaag
      361 gttatgccgc agcggtagga ggtggtttgc agtggaaaat tggcaacagc ttccgtttgt
      421 ttggcgagta ttactactct ccggattcgc tctccagcgg tattcaaagt tatgaggaag
      481 cgaatgctgg cgcgcgttac accattatgc gtccagtcag tattgaggcg ggttatcgct
      541 acctgaatct gtcgggtaaa gacggtaacc gcgacaacgc cgtggctgac ggcccgtatg
      601 ttggggttaa cgccagtttc tgatcccttc cggcgcggca ttctgtcgcg ccattcagtt
      661 tcttactacg ccttatctcc ctttgcgtta tagtgattcg ataacaaaag caggagataa
      721 ccatgttaaa agtggaaatg ctatccaccg gggatgaagt gttacacggg caaatcgttg
      781 acactaacgc tgcctggctg gccgattttt tctttcatca ggggctgcca ttatctcgcc
      841 gcaatacggt gggggataac cttgatgact tagtcaccat tcttcgcgaa cgtagtcagc
      901 acgccgatgt gctgatcgtt aacggcgggc tgggaccgac cagcgatgat ttaagcgcac
      961 tcgccgctgc gacagcaaaa ggtgaaggca tggtgctgca tgaagcctgg ctcaaagaga
     1021 tggaacgcta tttccacgaa cgtggacgag taatggcacc gagcaaccgt aaacaagcgg
     1081 agctgcctgc cagtgctgaa tttatcaata acccggtagg caccgcctgt ggttttgccg
     1141 tgcagcttaa tcgttgcctg atgttcttta ctcccggcgt accgtcagaa tttaaggtga
     1201 tggtcgagca cgaaatcctg ccgcgtctgc gcgagcgttt ttctttacca cagccgccgg
     1261 tttgtctgcg tttaactacc tttggtcgtt cggaaagcga tctggcacaa agcctggaca
     1321 ctctacaact gccgccgggc gtaacaatgg gctatcgctc ttcaatgccg attatcgaac
     1381 tgaaactcac cggaccggca agcgagcaac aggcgatgga aaaactgtgg ctggacgtta
     1441 aacgtgttgc cggacagagc gtgattttcg aaggcaccga aggactgccc gcgcagatca
     1501 gtcgcgaatt gcaaaaccgc cagttcagcc tgacgttgag cgagcaattc accggcggtt
     1561 tattggcttt gcaactttct cgcgcaggtg ctccattgct ggcgtgtgaa gtggttcctt
     1621 cacaggaaga aaccctggcg caaactgcgc actggattac agaacggcgg gccaaccatt
     1681 ttgccgggct ggcactggct gtttcgggtt tcgagaacga gcatctcaac tttgcgctag
     1741 ccacgccaga cggcactttc gctctgcgtg tgcgtttcag cactacacgc tacagcctgg
     1801 ctatccgtca ggaagtgtgc gcaatgatgg cactgaatat gctgcgccgt tggttaaacg
     1861 gccaggatat cgccagtgag catggctgga ttgaggttat tgagtccatg accttatctg
     1921 tctgattccg ccactctgtg ccggtttact gcaccggcac attccccctc cggcaaaact
     1981 tgtccaaccc tccctacggt gagcgtggtg ttggggccag tgtggcacgg gctgcgcgct
     2041 ggggacgcat tgagaattac atggcgcaag ttaacgattc actttgtctg ttggtgcagg
     2101 tggaaagtaa aacggcactg gataacctgg acgagatcct cgacgtcgaa gggattgatg
     2161 gcgtgtttat tggacctgcg gatctttctg cgtcgttggg ctacccggat aacgcagggc
     2221 acccggaagt gcagcgaatt attgaaacca gtattcagcg gatccgtgct gcgggtaaag
     2281 cggctggttt tctggctgtg gctcctgata tggcgcagca atgcctggcg tggggagcga
     2341 actttgtcgc tgttggcgtt gacacgatgc tctacagcga tgccctggat caacgactgg
     2401 cgatgtttaa atcaggcaaa aatgggccac gcataaaagg tagttattga tatcaaaggc
     2461 ccatggggat cggctgtggg cctgtgttaa ttagtggtta ttcgctgcca gatcggcttc
     2521 gcttagctgg gtggccgcga gcacctggtc acgatcaatg ccctgttcca gcatgcgcaa
     2581 agcaatttta atggcttgtt catgcatacc ttcctgccag ccaatttgat gcccttcctg
     2641 ccgtagccgt tcagcaatag tcattaatct ctccttttgt agtggtgaac gttcggcaat
     2701 ctcctcaata aaacgggtga aacgggaggt atcgccgcat tgcagcagat aattaaacag
     2761 tgtttgtagc tggctgtcat tagtgaagcc tctaaccaaa agcgtggtga tcctgtcgac
     2821 catgccgatt aaatcgcggt cgcgaatatg cttttgaatc agttccagca gagctatacg
     2881 ccgatgttgc atgatctcat cgtcaggtac gatggtgata tccaccaacg gaaaagcttc
     2941 ggtgtacaac tgccgggcaa gttgcggatc gtcaaactca tccagccagt taagtgagta
     3001 cgggtagggc gatgtttcgc catgataaaa cagcaatggc accaccagcg gaactctgtc
     3061 atagccttta tccaggtgac gctgcatggc ggcagtggca tagcgcatta gccgaaaagc
     3121 catattcttt tctgcagagc tttgatgttc aatcacgcag tagatatagc cgtcaccgtc
     3181 gctggtttcc acggaccaca aaacatccga gtagtaagcg cgtaaacttt tttcaataaa
     3241 actagtgggt tccaggcgta aggtttgcag gttgcaaagc ttgcgcagtg gttctggtaa
     3301 atgtatttcg agaaaatccc gtgcggtttc gggtgtgaac ataaaggttt taaacaccgc
     3361 atcatgcggg gaggatgttg ttgattcggt catggttgtc cgtcacctga tatttatgat
     3421 gacgcgacat tacgtctatc ccgggccttt gacatctgtc agtttcagcc tttgcgagac
     3481 attttcagag gaattttcaa agcgttgcaa cgcatacatg gtgcgtggaa tcgcgctcgc
     3541 aaaaatgccc gataagcaat gtgcttaccg ggcatcaaaa ggtcggttaa gccagcgcct
     3601 gggccagtag cgtaatcgga tgttcgcagc gaaggctcgt ggacatctca atctgccatt
     3661 tgcaggtttc gcagtcggtg atgaccagat ccgcgccgct ctcttctatc tggcggaaca
     3721 gtggcgcacc gatggcttgt gaggtggggt agttctcttt tttgaaaccg taagtacccg
     3781 caataccgca gcactgggaa tccagcaccg ttaactcaag ccccgggatt ttacgcaaca
     3841 gctccagggt gtagagcgtc cagcccattt tttccatatg gcacggagtg tgataaacca
     3901 ctttcagcgg cagcggtttc agcggtaacg ttttgccttc gtccagcttg cgccacagcc
     3961 agcgggttgc cagttcgata tgatcgcgca agcctttgtt gtcgacattc agcacttccg
     4021 ggtattcgtc gcgcagggca aatgtacagg ttgaggaggt ggcaatcacc ggaatgcctt
     4081 ttactcccac agcttcgcgg atcgactcta cattcgtaat tgcctgtttg cgtgctttat
     4141 cggtaaagcc gttggcgatt agcggtacgc cgcagcattt ttctttgctg agcagttgta
     4201 caccggtacc cattgcgttg agcactttaa ttaaatcttt acctaactgc ggatggttgt
     4261 agttaacgaa gcagccgtga aagaaagcga cctggtcttt atattgtgct tgctgagccg
     4321 ctatgctgcg ataccagcga cggaacgtgc cgaaggagta tttcggtagc gtgcggcgat
     4381 gatcgatttt taacgccgca tcaagcagct gccgcaccgg tttcagcgag gtagcggtgt
     4441 tgacgattgg tgcgaacggc gtggaaacgc tacccatcag gtcggtatga ctcaacacaa
     4501 aattacgcag cgacgggcgc gtggtgtcat attttgcccg cgcgcgctgg ataatatcgc
     4561 caatcttcac atcggacggg caggcgactt cacaacgttt gcagttgatg caatatttca
     4621 gcgcctcgtc atacagtgcg ccatctttca aacgcagacg ctcgccatcc ggcccggctt
     4681 gttttggccc tggataaccg ggattcaccc ggctcaccgg gcaggcggtg gtgcagacgg
     4741 tgcacttaat gcagttttcg aagctggtgt cgttcattgt tggcctcctg cgcgttgggc
     4801 aatctgttga gcggcatgta aagcactgac ggcacaaaca ccgccgccgc atccctgggc
     4861 gatgggatca aatccgccca gcaccgaacc gatggcaaac aggttctcaa tggtttgccc
     4921 tgcctgcgac gggcgtagcg tctcatcagt ggttacaccg aactgctgcc acggttgcgg
     4981 cgcaaaaaaa tcgcccttat accattcacc ccgcgtggcg gtttgtagca catcaaggcc
     5041 gagaatcggc tcgcgaatgc cgttacgttc cgctaccagt ccgccactaa agaaactgcc
     5101 gctggcgaga accgcgaaac gtggacgtag cggaatatcg gcgtgattgc gggtccagat
     5161 ttcattcact acgccatttt tacaggtcac ttttttcact tcatcgcccg gcatccacac
     5221 gccaccctgg cgcacaaact ggcgctgtaa ctggttttgc agacgaatgc ccagcacgga
     5281 aggcggcagc gttggcaaaa gcatcagtga acaaggtagt ttttcattca accaacgcca
     5341 cagtttgtta tcggccaggc cgaagcaggc gggcatcagg atcatttcgc aggtattggc
     5401 gacaggaata agcgcatcaa gtaacagcgg ccagttttct tcattatcaa ggaaacgggc
     5461 gatattcaca gcgcgaaatt cggtggcgtt attgcgcagg acatccagtt ccggcagctc
     5521 tatttctgcg gtttcaaccg aaagatccag ttcgcgcaac gaagccgccg caagatgcgc
     5581 ctggaaatcc atcagcccgc ttatccccac tacacatatc ttcttcgcgg gtagcggcca
     5641 gacgggaact tctggcgaac ttaaccaggt tgcacgcaaa gtgccgagcg gggtaacgcg
     5701 ctgatgggcc agttccacgc tgccctgcaa ttgcgcaccg ctttcagcga tcaatgcctg
     5761 cgcctggcaa gcgagatcga gcacgcgttg tggttcgaga agactgtaag gatgggctgg
     5821 tgcctgctga cgcaaagatt ccagtccact gtgaatgtct gtcaccggtt gaccatcagg
     5881 cagatggctc agcaaatcca gcgaaccgga tgagaaatgc agcgcgcttt gaccacgcgt
     5941 gacaatggca cagcgcaggc cgtgtttttg cagttgcagg ccacagagta atccggcgag
     6001 gccgccgccc ataatgacag tatcaaagcg catctttctg ctccttctcc agaccacaca
     6061 gtccctgata aacccagcgg gtaaattcgc tttcgcgcag tgcgtctccc caggcgatgg
     6121 gttgcacgcc tttccagcgt tcgttaagga aggtggaaag ttgctcgata gattgcgcgg
     6181 acgtcgtgac gttaaaccgt tgcagcagtc cggcagcgcg gcaggcgcag agttcgccct
     6241 ggcaggtacc catccccaca cgggtacgac ggcgtaaatc cagcaggcta ttaacgttta
     6301 aattttctac cgcgtactga acttcacccg cagttaccgc ttcgcactca cataccaggc
     6361 tacgatgcag acggccttcg ctcagccagg caggcgtgcg atcgccatga cgataaaccg
     6421 cagaaccgcg cagcggggca ggcagggaga tgactttacg caaggtaact tcagcgggtt
     6481 cttgtgaacc aggcagtgcc agatcggcag tcgtacaggg gcgcgtgttg cccagtttgc
     6541 ggcataccgc gtcggtagcc cattcagcca tcagccgata ggtcatcagt ttgccaccgg
     6601 tgatggtgat aaatccgtcc agaccatcgc gttcagcatg gtcgagcagc acgatgccac
     6661 ggctgacgtt acgtccgctc gggtcgtcat cgctggcaac cagcgggcgc acgccagaat
     6721 aggcccgcaa aatgcgcgtt ttcgccatca cgggggccag tttttcccct tcacgcagca
     6781 gaatatcaac ctcttctgcc gtcactcgat tatcgtcaat ctcgttgtaa tcaatacgta
     6841 aagaggtggt gccaatcagc gaaatggtat cgccaggcac cagaatatcg gcgtcggaag
     6901 gtttacggca gcggttgatc acatgctggt taatgcggtg atccatgatc agcagcgatc
     6961 ctttcgccgg gaacatgcga atgcgcagat cggcatattc ggcaatgtgt tgcccccaga
     7021 tcccagcggc attaaccacg acaggtgcat gaagggcctg agtttcgccg gtgagatggt
     7081 tacgtacacg aacaccgcac accgtcgcgc cttcacgaat cagccccgtg acttcatgag
     7141 cggtaaggat aacggcaccg tgttctttgg catccagcat gtttgctgcg gtcagacgaa
     7201 atggatcaac ggtgccatcc ggaactttca ccgcgccaat cagtgccggg ttaacggcag
     7261 gttcgataat gcgcgcttgc tgcgggtcta tagcttctgc gctgatccct gcttcttcgc
     7321 aggcgcgaat aaaagtggcc tggaatgaga ggtcatcttc cggcagggtg ataaacaggc
     7381 cgttggttgg ttcaacgcag tgacgtgcaa tgcgtttcag gatctggttt tcactaatgc
     7441 attcgcgggc cgattccgca tcggttaccg catagcgcgc accgctgtgc agcaggccgt
     7501 ggttacgccc ggtggcaccg gttgcgatgt cgtggcgctc aaccaaaatc acgcgcagcc
     7561 cgcgcagggc acagtcgcgg gcaatcccgg ctcccgttgc gccgccacca atgataatca
     7621 cgtcacttga ttgcgagtcg cgagttttca ttgttatccc tctgaagttc gttttttacc
     7681 atttagccat agtaaaaaca tgaattgttt gatttcgcgc atattcgctc ataattcgaa
     7741 agtgaaacgt gatttcatgc gtcattctga acattttgta aatcttattt aataatgtgt
     7801 gcggcaattc acatttaatt tctgaatgtt ttcttaacat cgcggcaact aaagaaacgg
     7861 caggttctcc cactgaatca gtctgttaat cataaataag accacgggcc acggaggctt
     7921 tcaatgttga gtatttttaa accagcgcca cacaaagcgc gcttacctgc tgcggagatc
     7981 gatccgactt atcgtcgatt gcgctggcaa attttcctgg ggatattctt tggctatgcg
     8041 gcttactatt tggttcgtaa gaactttgcg cttgctatgc cttatctggt cgagcaggga
     8101 ttctctcgcg gtgatttagg tttcgcgctt tcggggatct cgatcgctta tggattttcg
     8161 aaattcataa tgggttcggt atcagatcgc tcgaatccgc gcgttttcct ccccgcaggt
     8221 ttgattctgg cggcggcagt gatgttgttt atgggctttg tgccatgggc gacgtcgagc
     8281 attgcggtga tgtttgtact tttgttcctc tgcggttggt tccaggggat ggggtggccg
     8341 ccgtgtggtc gtactatggt gcactggtgg tcgcagaaag aacgtggcgg cattgtgtca
     8401 gtgtggaact gtgcgcacaa cgttggtggt ggtattccgc cgctgctgtt cctgctgggg
     8461 atggcctggt tcaatgactg gcacgcggcg ctctatatgc ccgctttctg cgccattctg
     8521 gtggcattat tcgcctttgc gatgatgcgc gataccccgc aatcctgtgg cttgccgccg
     8581 atcgaagagt acaaaaatga ttatccggac gactataacg aaaaagcgga acaggagctg
     8641 acggcgaagc aaatcttcat gcagtacgta ctgccgaaca aactgctgtg gtatatcgcc
     8701 atcgccaacg tgttcgttta tctgctgcgt tacggcatcc tcgactggtc accgacttat
     8761 ctgaaagagg ttaagcattt cgcgctggat aaatcctcct gggcctactt cctttatgaa
     8821 tatgcaggta ttccgggcac tctgctgtgc ggctggatgt cggataaagt cttccgtggc
     8881 aaccgtgggg caaccggcgt tttctttatg acactggtga ccatcgcgac tatcgtttac
     8941 tggatgaacc cggcaggtaa cccaaccgtc gatatgattt gtatgattgt tatcggcttc
     9001 ctgatctacg gtcctgtgat gctgatcggt ctgcatgcgc tggaactggc accgaaaaaa
     9061 gcggcaggta cggcagcggg ctttaccggg ctgtttggtt acctgggcgg ttcggtggcg
     9121 gcgagcgcga ttgttggcta caccgtggac ttcttcggct gggatggcgg ctttatggta
     9181 atgattggcg gcagcattct ggcggttatc ttgttgattg ttgtgatgat tggcgaaaaa
     9241 cgtcgccatg aacaattact gcaaaaacgc aacggaggct aatggcatga aattgacgct
     9301 gaaaaacctt agcatggcga tcatgatgag cactatagtc atgggaagca gtgcaatggc
     9361 ggcggacagc aacgaaaaaa tagtcatcgc ccatcgcggt gccagtggat atttgccgga
     9421 gcatacgctg ccagcaaaag cgatggcgta tgcgcaggga gcggattatc tggaacagga
     9481 tttggtgatg accaaagacg accatctggt tgttctgcat gaccattatc tcgatcgtgt
     9541 tactgatgtt gccgatcgtt tcccggatcg ggcgcgcaaa gacggtcgtt actacgcgat
     9601 agatttcacg ctggatgaaa ttaagtcgct gaaatttacc gaaggtttcg atattgaaaa
     9661 cggtaaaaaa gtgcagactt atccggggcg tttcccaatg ggtaagtccg acttccgggt
     9721 gcacaccttt gaagaagaga ttgaatttgt tcaggggtta aatcactcta ccgggaaaaa
     9781 tatcggtatt tatccagaaa tcaaagcgcc gtggttccat catcaggaag ggaaggatat
     9841 tgcggcaaaa acgctggaag tgctgaagaa atatggttac accggtaaag acgataaagt
     9901 ttatttgcaa tgttttgatg ctgatgagct gaagcgtatt aagaatgagc tggaacccaa
     9961 aatgggcatg gagctcaatc tggtacagct gattgcctat accgactgga atgaaacgca
    10021 gcagaaacag ccggatggaa gctgggttaa ttacaactac gactggatgt ttaagccggg
    10081 tgccatgaaa caggtggcgg aatatgcaga tggtattggt ccggattacc atatgttgat
    10141 tgaggaaaca tcgcagccgg gtaatatcaa actcactggc atggtgcaag atgctcagca
    10201 gaataaactg gtagtgcatc cttataccgt gcggtcggat aaactgcctg aatacactac
    10261 tgatgtgaat cagttatatg atgctctgta taacaaagcg ggtgtaaatg ggctgtttac
    10321 tgatttccct gataaggcag taaaattcct taataaagag taatgctact gcaggatggc
    10381 gttcatgcgc catcctgtct catatcaaat ttttccattc accacgagtt gttccgtaca
    10441 cgtttgtagt aaaaacagtg gactgccttc taccggcttt tctttagcca acaacgaaat
    10501 ctgtcgatag aaggttggtt cgtgaggaac ggaacacaat tcacctgcaa tgcttttcag
    10561 cgttaactct ggttgtaagg cgatgcctaa tccctgtcga ataaacttca tcgctgtatc
    10621 cgactgatta aattcacatt ttatctactg ggcgcactat tagtggtagt ggctggtgga
    10681 atctatgcgc tgcaaatgaa gggtaaaagc ggtgtcacgc tagtagtggc aaaagaaatt
    10741 cattaatacg acacgtttca ttaagatttt cctcaggtaa ccacctatag tcattgtcgt
    10801 taataaaaat gacaggcgat agggtatggc agtttcagca aagtacgacg aatttaatca
    10861 ctggtgggca acagagggcg actgggttga agagcctaac tatcgccgta atggtatgag
    10921 cggggtgcaa tgtgtcgagc gcaacggcaa aaagctgtat gtaaagcgca tgacgcatca
    10981 tctgtttcat tccgtacgtt atccgttcgg ccgaccaacg attgtccgtg aggttgcggt
    11041 aattaaagaa ctggaacggg ccggtgtcat cgtgccgaag atcgtttttg gtgaagcggt
    11101 gaaaattgag ggtgaatggc gagcgttgct ggtgactgaa gatatggcgg ggttcatcag
    11161 cattgctgac tggtatgccc agcatgcagt atcgccttat tctgacgaag tacggcaagc
    11221 catgttgaaa gcagtggcgc tggcgtttaa gaaaatgcat agcattaatc gtcagcatgg
    11281 ctgttgttat gttcgccata tctatgtgaa aacagaaggt aatgcagaag ctggttttct
    11341 tgacctggag aaaagtcgtc gccgcttgcg tcgcgataaa gcgattaatc atgacttccg
    11401 ccagctggaa aaatatctgg agccaatccc caaagcggac tgggagcagg tgaaagcgta
    11461 ttactacgcc atgtaatcgt tgtaggccag ataagacgca taagcgttgt atctggcact
    11521 acaaccaatt cacatctcga tttcaatatc gccttttgcc cggcaacaac agggcaaaat
    11581 ttcccccggc tgaataaagg ctaacggttc ggcaatccag tcaacctgac ctgcaaccag
    11641 acgggtgcga caggagccgc agtaaccttc gcgacactga tactcaaccg ccacattgtg
    11701 ggattccagc gccgccagaa gggaagggtg ttcatcctgg cacagcagtt gtgtgccagt
    11761 gatgcgcagg gtaacgcggg ccatcagagt tggaagttac tcaaatcgtc ggtgtccact
    11821 tccgcgtcaa tctgcccgac cagataagaa ctgacttcca cttcctgcgg agcaacctgc
    11881 acgttatcag acaccagcca ggtgttgatc catgggatcg ggttggagcg cgtctggaac
    11941 ggcagatcca aaccgactgc ctgcatacgg atattggtga tgtattcaac gtactggcag
    12001 agaatgtctt tattcagacc aatcatcgaa ccgtcacgga acagataatc cgcccagtct
    12061 ttctcctgtt gagctgcctg aacaaacagg tcatagcact cctgcttaca ctcttcggca
    12121 atttccgcca tctcaggatc gtccgcgccg ctgcgcagca gattcagcat atgctgggtg
    12181 ccggtcaggt gcagggcttc gtcgcgggca atcaggcgaa taattttggc gttgccttcc
    12241 atcaattcgc gttctgcaaa ggcaaaggaa caagcaaagc tgacgtagaa gcggatcgct
    12301 tccagcgcgt taacgctcat caggcaaaga tacagttttt tcttcagctc gcgcaggcta
    12361 acggttacgg ttttaccgtt aacggtgtgg gtgccttcgc ccagaaggtg ccagtagctg
    12421 gtcatttcga tcagttcatc gtaatagctg gagatccctt ccgcacgttt ctggatctgc
    12481 tcgttggtga cgatatcgtc aaacacaaca gacggatcgt taacgatatt acgaatgata
    12541 tgagtatagg aacgggaatg aatcgtttct gagaacgccc aggtttcgac ccaggtttcc
    12601 agttccggaa tagaaataag cggcaatagc gccacgttcg gactacgacc ctgaatggag
    12661 tccagcagcg tctgatattt caggttgctg ataaagatgt gtttttcgtg ctccggcagc
    12721 gcctggtaat cgatacggtc gcgggagacg tcaacttctt ccggacgcca gaagaaagag
    12781 agctgctttt cgatcagctt ttcgaagatg tcatattttt gctgatcgta gcgagccacg
    12841 ttgaccggct gaccaaaaaa catcggttct ttaagctgat catttttcgt ctgtgaaaag
    12901 gtggtatatg ccatgagtgt gtcctgtggt tttattaccc ctcaccctaa ccctctcccg
    12961 caaggggaga ggggacggtt tggtgcaaag gctgggggat tatcagatct tacatgcgcc
    13021 gctttcgcag ccatcgtcct ggattgacgg caccagatcg tcctgcgcgt cttcagcgcc
    13081 gtcacgggtg ttctgataat acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc
    13141 tttcagcaac tgctgcatcg gcacttttcc tgacgggaaa cgtgacggat cgtagttggt
    13201 gttagcagag atcgactgat cgataaattt ctgcatgata cctaccagtt gcaggtagcc
    13261 atcgttaccc ggcatttccc acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg
    13321 caccacctgg cgcaaaatac cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg
    13381 ttcaataccg ttagtggcgt tagagatctg cgaagaggtc tcggacggca tcagggcaga
    13441 aagcgtggag ttacgcaggc cgtgcgtttt gattgattca cgcaatgctt cccagtcgta
    13501 atgcagcggc tcattagcga tggcatccag atctttctta taggtatcga tcggcaggat
    13561 ccctttcgcg taagtggttt cgttaaacca cgggcacgcg ccttgctctt tagccagctc
    13621 attagacgct ttcagcaggt aatactgaat cgcttcgaag gttttatgcg tcaggttgtt
    13681 ggcgctgccg tcggagtagc gtttaccgtg cttcgccaga tagtaagcga agttaatcac
    13741 accaataccc agcgtacgac gccccatcgc tccacgtttg gcggccggga tcgggtaatc
    13801 ctgataatcc agcagcgcgt caagtgcacg aaccgccaga attgccagct cttccagttc
    13861 atccaggcta ttaattgcgc ccaggttgaa agcagacagc gtacacagcg cgatttcacc
    13921 gttctcgtcg ttgacgtcgt tcagcggttt ggtcggcagg gctatctcca ggcacaggtt
    13981 agactgacgc actggcgcga tggccggatc aaacgggcta tgggtattgc agtggtcaac
    14041 gttctgaata tagatacgac cggtagacgc acgttcctgc atcatcagcg agaacagctc
    14101 aacggctttc acacgctgct tgcggatgct gtcgtctttc tcatatttgg tatacaggcg
    14161 ttcaaactct tcctgatcgg cgaagaatgc gtcgtacagc cccggtacgt cggacgggct
    14221 gaacagggtg atatcttcac ctttcagcag gcgggtatac atcagtttgt tgatctgtac
    14281 cccgtagtcc atatggcgca cgcggttgcc ttccacacca cggttgtttt tcaacaccag
    14341 caggctttcc acttccagat gccacatcgg gtagaacagc gttgccgcac cgccgcgcac
    14401 gccgccctga gagcaggatt tcaccgctgt ctggaaatgt ttgtagaacg gaatacagcc
    14461 ggtatggaac gcttcaccac cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt
    14521 gatgccgatc ccggcacgct gggaaacgta tttcacaatc gcgctggagg tagcgttgat
    14581 ggaatccaga ctgtcaccgc actcgatcag tacgcaggag ctgaactgac gagtcggggt
    14641 acgcacgccg gacatgattg gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta
    14701 aaaacgcttc acatattgca ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc
    14761 aaccagaata taaaggaact gggcgctttc gtagatttcg ccggtcacgc ggttctgtac
    14821 cagatatttg ccttccagct gcttaacggc agcataagag aaggtcatat cacggtcgtg
    14881 atcgataaac gtgtccatct gcttgaactc ttcttccgtg tagtcttcca gcagatgatt
    14941 atcgtatttg cccatctcga ccattttcac cacgtggtcg tacagcgcag gcggctcaaa
    15001 ctggccgtag gcttttttac gcaggtggaa gatcgccagg cgcgcggcga gatactgata
    15061 atccggcgca tcacgggaga tcaggtctgc ggccgccttg ataatggttt cgtggatgtc
    15121 agaggtcttg ataccgtcat aaaactgaat gtgggagcgc agctcgacct gggaaatcga
    15181 aacgttatgc agtccttctg ccgcccaatc cagaacgcga tggattttgt cgagattgat
    15241 gcgctctgtg ctaccgtcgc gctttgtcac cagcagattc tgattcatgt atgtcgtacc
    15301 tgtttttgga attctttccg caatagtttc atgcagacta cctgtagtaa gggaagctgc
    15361 ttagaacact atatataggg ggtatgtttg ggattcactg caagatagtg tgaaaatggc
    15421 cctcttgcaa gtgcataact ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac
    15481 cttaggtcag acaaggtgtc cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa
    15541 ctgttcggcg atttcttgaa cggcagaata ggttggataa attgatatag atggtttatt
    15601 aatctttttg ctaatgattg caaaaattga tctaagcacg agtcat
//
LOCUS       DAPGWD010000096        15521 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPGWD010000096 DAPGWD010000000
VERSION     DAPGWD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.097"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            153..1736
                     /locus_tag="RHX88_002954"
     CDS             153..1736
                     /locus_tag="RHX88_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV2231621.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1811..2149
                     /locus_tag="RHX88_002955"
     CDS             1811..2149
                     /locus_tag="RHX88_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2231622.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKS
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2139..2429
                     /locus_tag="RHX88_002956"
     CDS             2139..2429
                     /locus_tag="RHX88_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDV2231623.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEIQYPHAA"
     gene            complement(2482..4335)
                     /gene="asmA"
                     /locus_tag="RHX88_002957"
     CDS             complement(2482..4335)
                     /gene="asmA"
                     /locus_tag="RHX88_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDV2231624.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4357..4938)
                     /gene="dcd"
                     /locus_tag="RHX88_002958"
     CDS             complement(4357..4938)
                     /gene="dcd"
                     /locus_tag="RHX88_002958"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDV2231625.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5030..5671)
                     /gene="udk"
                     /locus_tag="RHX88_002959"
     CDS             complement(5030..5671)
                     /gene="udk"
                     /locus_tag="RHX88_002959"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDV2231626.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5989..9306
                     /locus_tag="RHX88_002960"
     CDS             5989..9306
                     /locus_tag="RHX88_002960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV2231627.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGGLVVLLTAGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNIESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9345..10202)
                     /gene="alkA"
                     /locus_tag="RHX88_002961"
     CDS             complement(9345..10202)
                     /gene="alkA"
                     /locus_tag="RHX88_002961"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDV2231628.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            10336..11688
                     /gene="yegD"
                     /locus_tag="RHX88_002962"
     CDS             10336..11688
                     /gene="yegD"
                     /locus_tag="RHX88_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2231629.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSTAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11700..13640)
                     /gene="yegI"
                     /locus_tag="RHX88_002963"
     CDS             complement(11700..13640)
                     /gene="yegI"
                     /locus_tag="RHX88_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDV2231630.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTKNHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVLTKMAAKRHRLESTLTVGATELQRFRLQAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13637..14398)
                     /gene="pphC"
                     /locus_tag="RHX88_002964"
     CDS             complement(13637..14398)
                     /gene="pphC"
                     /locus_tag="RHX88_002964"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HDV2231631.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14395..15054)
                     /locus_tag="RHX88_002965"
     CDS             complement(14395..15054)
                     /locus_tag="RHX88_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV2231632.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15275..15334)
                     /gene="ibsA"
                     /locus_tag="RHX88_002966"
     CDS             complement(15275..15334)
                     /gene="ibsA"
                     /locus_tag="RHX88_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDV2231633.1"
                     /translation="MMKHVIILVILLVISFQAY"
BASE COUNT         3752 a         4064 c         3955 g         3750 t
ORIGIN      
        1 ttattattac cacttttaat tcagggataa tgtgaggtta ttaccctcaa ataatatgag
       61 ataaatagtg ctgcaacatt gcatttttgc cccgatttat ccatgatcga attgtgacat
      121 ttgtcataca acgaataggt tttgtactta ctatggaatg gattgccgat ccgtctatct
      181 gggccgggtt aatcacgctg attgtgatcg aactggtcct cggcattgat aacctggtct
      241 ttattgccat cctcgccgaa aaactaccgc cgaagcagcg cgaccgcgca cgggttaccg
      301 ggctgctgct ggcgatgtta atgcgcctgt tactgctggc gtcaatctcc tggctggtca
      361 ccctgacgca accgctgttc agcttccgct cgtttacctt tagcgcccgc gacttaatca
      421 tgctgtttgg tggtttcttc ctgttgttca aagccacgat ggagctgaac gaacggctgg
      481 aagggaaaga cagcaataat cccacacaac gcaaaggtgc gaagttctgg ggggtggtga
      541 cacaaattgt ggtactggac gccatcttct cactcgactc agtgattacc gccgtcggga
      601 tggtcgacca tttactggtc atgatggccg ccgtggttat cgctatcagc ctgatgttga
      661 tggccagcaa gccgttaacg caatttgtta acagtcaccc gaccatcgtt attctctgct
      721 taagcttcct gttgatgatt ggctttagcc tggtggcaga aggtttcggc ttcgtcattc
      781 cgaaaggcta cctgtacgct gccattggtt tctcggtgat gatcgaggcg ctcaatcagt
      841 tggctatctt taaccggcga cgttttcttt ccgctaacca gacgctacgc cagcggacta
      901 ccgaagcggt aatgcgcctg cttagcgggc aaaaagaaga tgcggaactg gacgccgaaa
      961 ccgcgtccat gctggtggat catggtaacc agcagatctt taatccgcag gaacggcgga
     1021 tgattgagcg ggtacttaat cttaaccagc gtactgtcag cagcattatg acgtcgcgcc
     1081 acgatattga gcatatcgat ctcaacgcgc cggaagacga gatccgccag ttgctggagc
     1141 gaaatcagca tacgcggctg gtggttaccg acggtgatga cgcagaagat ttgctcggtg
     1201 ttgttcacgt tatcgacctg ctacaacagt cactgcgtgg cgaaccgctc aacctgcggg
     1261 tgttgattcg ccagccgctg gtgttcccgg aaaccttgcc gttgttacct gccctggagc
     1321 agttccgtaa tgcccgcacg cactttgctt ttgtggtgga tgagtttggc tcggtggaag
     1381 ggattgtgac attaagtgac gtcactgaaa ccattgccgg taacttaccg aacgaagtgg
     1441 aagagatcga cgcccgccat gatattcaga agaatgcgga cggttcctgg acggcgaatg
     1501 gtcatatgcc gctggaggat ctggtgcaat atgtgccgct gccgctggat gaaaaacgtg
     1561 aatatcacac cattgccggg ctgttgatgg aatatttaca gcgtattcca aaacctggcg
     1621 aagaagttca ggtgggggat tatctgctta aaacgttgca ggtggaaagc catcgcgtgc
     1681 agaaggtgca gattattccg ctacgtaagg atggcgagat ggagtacgag gtgtgataaa
     1741 acatcaaatg cgcaggttca aactgcgcat ttgatttata caaaatttag tataatctaa
     1801 ggatggagta atgagaaaaa agctggcttt tcttgatacc agtcttgacg atttacgcgc
     1861 atttccagaa tcgtctcgac aggaaattgg ctatcaactc gacaggatcc aacaagggtt
     1921 aaacccatac gactggaaat ctttttcaac tatcggccct ggtgtgcgag agattcgtac
     1981 tcgcgatgct gatgggatct atcgcgtcat gtatgtcgca aaatttgaag aagcggttta
     2041 cgtattgcat tgttttcaaa agaaaacaca gactaccagt caatcggata ttgatttagc
     2101 caaacgacgc tataaagaac ttgttcagga gcggaaaaat gaaaattgaa acctttgata
     2161 gtgtgtggga tgcggtgagt gacacccctg aacaagcgga aaatatgcgg atccgtgccg
     2221 agcttgtaac gatcattaat aactggattg aacaacaagg gtttagccag gctcaggcag
     2281 cttctgcttt aggggtcact caacctcgca tatctgaact tgccagaggt aagatccaga
     2341 tctttagtat cgataaattg atcaccatga tggctcatgc aggactacat attcaacgta
     2401 ttgaaattca atatccacat gctgcttagt gctataaata cagatggcga caaattgacg
     2461 ccgccatctg cataacttgt cctacatctt ctccagcaac ttcttcacat ctttgccatt
     2521 gcgggaatct ttattccgct cggcccagtc attcaggcga cgtttcgctt cgtcctgtag
     2581 atgtttgcgc agtaactgat ccacttgcag actgtaattg agttgctgcc agttgccata
     2641 aacccgcagc ggtactggcg tttctttgag gaaatcaatc agtttgcttt ccccgttcca
     2701 gccacccacg acccgaatat caaactgggt gtcgcaggtt tgatctgcca gattcaacat
     2761 gccttcccct gtcagcgcca gcacaggcga ttgaccttgc atgtcgttta acgtcacgac
     2821 gccatccttc aacgtcaaat cggtggtaaa gcggtcaaga cgcgttacgt tatcgaagtt
     2881 ttcagcggcc ttcacatcac caccattacg ttctaccgct tgctgaatca tctgctggaa
     2941 gttcatccct tccatgcgcg tgtcggtcat ttcgacatgt gcctgtcctt gccagttgtg
     3001 gcggaatgcg tcggcatcta tgtcagcacc ggagaaatca ccagccagtg acatttttcc
     3061 ggtcaacgaa atcggatagt taaacgcctt cagaatggta ccaatctcaa cgttttccag
     3121 ccgtggctgg aagtttatcc gcggatttat tgatgtcgcg tccagcgtgc ccggcagtga
     3181 aacctgtcca ccgttaagct tgccctgcag ttgagtaatt tccagcaaac ccgacttgtt
     3241 ggtcatttgc gtggcaacat ctgtaaaatt cattccgcgc cagcgcacgt tactggcctg
     3301 caacaaaata tcagccgtaa agccttgcag tccctgatat gccggttcat caatacgcga
     3361 agaaatgacc gggcgcggca atgttgattg gctctgcccc tgctgcgcgg caccgttttc
     3421 accattcgct gtttcattaa gcgggatgag gttgtcaaga ttcagttgcg ggaattgcag
     3481 tctcagctgc cattcaggtt tctctgtcag cgtgacctgt gcttgcccgc tcagcgtgct
     3541 gtcattggcg gtcaagctga tttggttaaa tgaaaggcgt ttatgtgact cctgccactg
     3601 ggcctggaaa ctcccctgcc cctgaatacc ttgttttggt aaatctgcac cctgcaactg
     3661 ccagttaatt tgttcaatag ccgccgttaa atcatgcgga taatcagaag catctaccgt
     3721 accgttaagg gatatcgtga gatcgcgctg atcgcgatta acccgcccgg agaactcaaa
     3781 tgaaccacga tgttggggat cttgttccat ttgcaggcga atattgcgga ttgtcacctg
     3841 ctcgtcatct tcatgctgga acaccagcac gctgtccgcc accttaagac tggatatatc
     3901 aaacgaccat ccgcgatcgt ctgacagatc cggcaaggta ttgtcgcgcg gtgcaaccgg
     3961 agcgtcttca ctgcgcaccg cttccgtctg cggcgtcagt tgaatcactg ccccttttag
     4021 catcacctgc ttaacgctca gttgatgact cagtagtggt aaaagcgcca cgtccagacg
     4081 catgttgtcg gcgcgaacca gtggctggct tgcgccctgg gcggtgagag acattcgccc
     4141 ggagaggata ctaagctgcg gccagacgtg ccaacgcagt ggcccgtcga gctgcaattg
     4201 ataaccgcta cgtgcagcaa cttgcttgac catatagtcg cggaaatcat tcggattcac
     4261 cagcaacact aacgcagata acccggccac cagcacgacc aggagtatca tcagcgtcgt
     4321 cagaaatcgt ctcatggtat cctcaatggg cctgaattag tctttatcga ttcggctggc
     4381 taccgcgccc tgctggttgc gatatttcgc atcttcacgg cggttgtaag gtcgcgccgc
     4441 cgggccggaa agcggctcaa agctcagcgc accaattaac atgcccggac gcagcgccag
     4501 cggcagctta ccggagttgt agaactccag cacaatgcaa ccagaccagc ccggatcgat
     4561 acggtgcgcg gtgacgtgca ccatcagccc cagacgcgcc aaagaggaac gcccgtccag
     4621 ccagcccacc agatcggctg gcagcgtcac cgactccagc gtcaccgcca gcgccagctc
     4681 tcctgggtga agatagaacg cctcgccctc gtcgagaacg atctcatcgc tcatcacgcg
     4741 gtcaagcgcg gcgctcactt catctttggg accgctcaga tcgataaacg ctgccgtgtg
     4801 accacggaag gtacgaaatt tattgcccag gcgtacatcc accgtcgcgc cgttaatacg
     4861 ctccactggc ggacgtgggt tgatcgacaa acggccttca tcaagccagg cttcaatatc
     4921 tcggtcacac agacgcatgg cattttctcc tttcgcgcat cactccctta acgccaatta
     4981 cgtcaagggc ataccaggtt atcactgaac ggtacacaat ttatcaagct tattcaaaga
     5041 actgacttat tttcgctttc aatatatcga tcgcgatgcg gtttttcccg ccgcgcggca
     5101 cgataatgtc cgcatattgt ttagaaggct caatgaattg caggaacatc gggcgcacgg
     5161 ttttttgata ttgcgccatc acggaatcca ttgaacgccc acgctcgtta acgtcacgct
     5221 tgatgcggcg catcaggcag atatccagcg gggtatcaac gaaaatggag aagttaagtt
     5281 cgtcacgcaa acgcgcatcc gtcagcaaca aaatgccttc gagaatgatg accttcttcg
     5341 gctcaaccgt caccgtttct ttcatacgcg tatgttcaac atagctgtaa accggcaggt
     5401 caattgccga gccgcgtttc aacgcttgta aatgctcaag caacaggctg tgatccatcg
     5461 cgctgggatg gtcgtagttg gtcttaacgc gttcttccat cgacagatgg ctttgatctt
     5521 tgtaatagca gtcttcggga attacgccga tgtgttcatc accgacttgc tcacgtaatt
     5581 cacgataaag ggtactggca ataagactct tgccggaagc cgatgcgcca gcgataccga
     5641 taatgacgca ctgatgagac tgatcagtca tatatttagc gacctgatta acctggatgt
     5701 taggaagggg gcgacgaagc gccaaacgcg gcaattatag ggatttcatc cgtctgatac
     5761 cagtcgaata gcgttgccgc gcgctcagag ttaattgttg acaaagaatt cccggtggca
     5821 aattacgttg atcagtttta tgtaaggtaa aaaatgttat acgcagttgc gcaaattatc
     5881 cgcctttacg tcactttatg agcaattcgc atataaaatg taaaactttt gtactagcat
     5941 aaacacagaa acgaatactg gcgacctggt cttgcggata aagcggtaat gagcaaacaa
     6001 tcacagcatg tattaattgc cctgccccac ccgctgcttc acctggtcag tttaggttta
     6061 gtctcgttta tctttaccct tttctcgctt gagctttcgc agtttggcac ccaactcgcc
     6121 ccactgtggt tcccgacgtc catcatgatg gtggcgtttt atcgccatgc cgggcgcatg
     6181 tggccgggaa ttgcgctgag ctgttcgctg ggaaatatcg ccgcatccat cctgcttttt
     6241 tccaccagct cgctgaacat gacctggacg accatcaata ttgttgaagc cgtggtcggg
     6301 gcagtgctgc tacgtaaatt gctgccgtgg tataacccct tgcaaaatct ggctgactgg
     6361 ctgcgtctgg cactcggcag cgccattgtt ccgcctctgt tggggggggg tctggttgtc
     6421 ctgctgacgg ccggagacga tcctctcagg gcatttttga tatgggtact gtcagaatcc
     6481 atcggcgctc tggcactggt gccgctggga ttgttattta aaccacacta tctgctgcgc
     6541 catcgcaacc cacggttgct ttttgagtcg ctgctcacgt tagccatcac actgacgtta
     6601 agctggcttt cgatgctgta tctgccgtgg ccttttactt tcattattgt gctgttgatg
     6661 tggagcgccg tgcgcctgcc acgaatggaa gcctttttga tcttccttac cacggtgatg
     6721 atggtgtcgc tgatgatggc cgcggatccc tccctgcttg ctacgccgcg tacatacctg
     6781 atgagccata tgccgtggct accgtttttg ctgatcctgc tgcccgccaa catcatgacg
     6841 atggtgatgt atgcctttcg tgcggaacgc aaacacattt ccgaaagcga aacccgtttt
     6901 cggaacgcca tggaatattc cgctatcggt atggcgttag tgggcaccga gggacaatgg
     6961 ctgcaaacca acaaagcgct ctgccagttt ctcgggtaca gtcaggaaga gctgcgcgga
     7021 ctcacctttc agcaactgac ctggccggag gatctcaata aagatctcca acaggttgaa
     7081 aagctgataa gcggtgaaat aaacacctat tcaatggaaa aacgctacta caaccgcaat
     7141 ggcgatgttg tctgggcgtt gcttgccgtc tcactggtgc gccacacgga tggcacgccg
     7201 ctctatttta tcgctcagat tgaagacatt aacgagctaa aacgcaccga acaggtgaat
     7261 cagcaactta tggagcgcat cacgctggct aacgaagcgg gcgggattgg catctgggag
     7321 tgggagttga agccgaatat ttttagctgg gataagcgga tgttcgagct gtatgaaatt
     7381 cctccacata tcaaaccgaa ctggcaggtg tggtacgagt gcgtgctgcc ggaagatcgc
     7441 cagcatgccg aaaaagtgat tcgtgattcg ttgcaatcac gctcgccctt taaactggaa
     7501 tttcgcatta ccgtaaaaga cggtattcgc catatccgcg ccctcgccaa ccgggtactg
     7561 aataaagaag gcgaagtcga acgcctgctc ggcattaata tggatatgac tgaggttaaa
     7621 cagcttaacg aggcattgtt tcaggaaaaa gagcgcctgc acataacgct ggactccatc
     7681 ggcgaagccg tggtctgtat tgatatggca atgaaaatta cctttatgaa tccagtggcg
     7741 gagaagatga gcggctggac gcaggaagaa gcattaggtg ttccgctcct gacggtgttg
     7801 catattacct ttggcgacaa tggtcccctg atggagaaca tttacagtgc cgacacctca
     7861 cgttccgcga ttgaacaaga tgtggtgttg cactgccgga gcggcggcag ctacgacgtg
     7921 cattacagta ttacgccgtt aagtactctg gacggcagca atattggttc ggttctggtg
     7981 attcaggacg ttaccgaatc acgcaaaatg ctgcgccagc tgagctacag cgcctcccat
     8041 gatgcactga cgcatctcgc caatcgcgcc agttttgaga agcaactgcg tatgctgctg
     8101 caaacggtaa acagtacaca tcagcgacat gccctggtgt ttatcgatct tgatcgcttt
     8161 aaagcggtga atgacagcgc cgggcatgcg gcgggcgacg ctttgctgcg cgaactggcg
     8221 tcgttgatgc tgagtatgct gcgctccagc gacgtgctgg cgcgactcgg tggtgatgaa
     8281 tttggtctac tgttgccaga ctgtaatatc gaaagtgcgc gttttatcgc tacacgcatt
     8341 atcagtgccg tgaatgatta tcactttata tgggaaggcc gtgtgcatcg ggtaggtgcc
     8401 agtgccggga ttaccttgat tgatgacaac aatcatcagg cggcagaagt gatgtcgcag
     8461 gctgatatcg cctgttatgc ctccaaaaat ggtggacggg gccgggtgac ggtttacgaa
     8521 ccacagcaag ctgccgcaca tagcgaacgg gcggcgatgt cgcttgatga acagtggcgg
     8581 atgattaaag agaatcagtt gatgatgatg gcccacggtg tcgcttcgcc gcggatcccg
     8641 gaagcgcgta atttgtggct gatttcgctt aaactctgga gttgcgaagg cgagattatt
     8701 gatgaacaaa aatttcgtcg tagcttcagc gatccggcac ttagccatgc tcttgaccga
     8761 cgggtattcc acgaattttt ccagctggcc gcaaaagcgg ttgccagtaa aggcataagc
     8821 atcgccctcc ccctttccgt tgccggtttg agtagcgcca cgctggtgaa tgatctgctt
     8881 gagcagctgg aaaatagccc tctaccacca cggttattac acctgattat tccggctgaa
     8941 gcgattttag atcacgcaga aagcgtgcaa aaactgcggc tggcgggatg tcggatagtg
     9001 ctcagccagg tgggccgcga tctgcaaatc ttcaactcgc tgaaagcgaa tatggcagat
     9061 tacctgctac ttgatggtga gttatgcgcc aacgtgcagg gtaatttgat ggatgagatg
     9121 ctgattacga ttattcaggg gcacgctcag cgactcggga tgaaaaccat cgccgggcca
     9181 gtcgttttac ccttagtgat ggatacgctt tctggcatcg gcgtcgatct gatttatggt
     9241 gatgtgattg ccgatgccca accgctggat ttgctggtga atagcagtta tttcgcgatt
     9301 aactgaggga gcgtttgccc attgcctgat gcgacgtatc aggcctacaa ttcgtccgtt
     9361 ccgtctggtt gccagccttc cgtataccag atatgcaaca gcgcataaga acgccagggc
     9421 ttccagcgct cggcataacg gcggatttgt gccggtgtca tccccggaaa tcgctgttta
     9481 atcagataat catccggcag aaaaacatct ttcgcctgcc agccacgcaa agcgaaataa
     9541 ttcgccgtcc agcgcccgat acccggaaaa gtttgcagcg ttttcatcgc ctgctccaca
     9601 tcgcccggaa ttgtcattgg taagctgccc tccagcgccg catttgccag atgaatcagt
     9661 gcctctgccc gtttcaacgg catgcctaac gcttttaatg cctgcgggtc ggctgctgcc
     9721 agccgctgag gcgtggggaa gcagatatac tccggaaaat catccagccg ttcgccataa
     9781 agctgtacaa ctttggcagt caattttgcc gccatcgcca cgctcaccag ttggcctaaa
     9841 atcgcccgca caccctgctc aaaagcatca atacagccgg gtaaacgcaa tccgggccgc
     9901 gccgcgccta acttgcccaa cgcaccgtta acaatctgcg ggttacattg cagatcaaac
     9961 aggcggctca ttttcgccag acactcagcg gcaacgggtt ctaaacctgc acttaaattt
    10021 atgtgcagag tatggcgggc tatatccgga atagcagtca ccacgccgcg atattcgccc
    10081 accgccagac tacgggcata ataatcctca gcgaccgttt ccacaccact caccgcacgg
    10141 gcggcgagaa atcccaacat ccacgaccag tcatacggcg gctgccagtt cagggtatac
    10201 atcgcatctc cttattcatc cgctttcagc ataaacgtta ttcagacgct acgctttgct
    10261 ttcatattcc ggttgtcgcg acggcaacat ttcgctaaag tcacgcccct tcttcaccgg
    10321 catggggatt aattcgtgtt tattggtttt gattacggta cagcaaactg ttcagtggcg
    10381 gtcatgcgtg acggtaaacc gcaattactg aaaatggaaa acgacagcac gctgctgcct
    10441 tcaatgcttt gcgcgccaac gcgtgaagca gtaagtgaat ggctgtaccg ccatcatgat
    10501 gttccggcag acgacgatga aacgcaggcg ctgttacgtc gggcgattcg ttataaccgc
    10561 gaagaagata tcgatgttac ggcgaaaagc gtgcagttcg gtctttcctc actggcacag
    10621 tacattgatg atccagaaga agtgtggttt gtgaaatcac caaaatcgtt cctcggtgcc
    10681 agcggcttaa aaccgcagca ggtagcgctg tttgaggatc tggtctgcgc aatgatgttg
    10741 cacattcgcc agcaggcgca ggcgcagctg ccagaagcga ttactcaggc ggtgattggt
    10801 cgcccgatca acttccaggg gctgggcggc gatgaagcaa acgcccaggc gcaagggatt
    10861 ctggaacgcg cggcgaagcg tgccggattc agggacgtgg tattccagta cgagccggtc
    10921 gcggctgggc tggattacga agccaccttg caggaagaaa aacgggtgct ggtggtggat
    10981 atcggcggtg gtacgactga ctgttcattg ctgctgatgg ggccgcagtg gcgttcgcgt
    11041 ctcgatcgtg aagccagcct gctgggtcac agtggttgcc gtattggcgg taacgatctg
    11101 gacatcgcac tggcgtttaa aaacctgatg ccgttactgg gcatgggtgg cgaaaccgaa
    11161 aaaggcatcg ccctgccgat tctgccgtgg tggaatgcgg ttgccatcaa cgacgtacct
    11221 gcgcagagtg atttctacag tagtgccaac ggtcgtctgc ttaacgatct ggtacgcgat
    11281 gcccgcgaac cggaaaaagt ggccctgcta cagaaagtct ggcgtcagcg tttaagctat
    11341 cgcctggtgc gtagcgcaga agagagcaaa attgctcttt ccagcactgc ggaaacccgc
    11401 gcctcactgc cgtttatcag cgatgaactg gctacgctga ttagccagca agggctggaa
    11461 agcgccctca gccagccact ggcgcggatt ctggaacagg tgcaactggc cctggataac
    11521 gcccaggaaa aaccggacgt tatctacctg accggcggta gcgcccgttc tccgctgatt
    11581 aaaaaagcgc tggcagaaca gttgccgggc attccgattg caggcggcga tgactttggc
    11641 tccgtcaccg ccgggctggc tcgctgggcg gaagtggtat ttcgttaaaa gaaaatggct
    11701 cagcagcggc ttaaatctgc ctgcgcctgg gccagttttt ctgctgcctg acgtaacggt
    11761 tccatcaacg gcatggtccg tgctggagct tgaaggcgga atcgctgcaa ctctgtcgcg
    11821 ccgacagtca acgtcgattc cagccgatgt cgtttggcgg ccattttagt cagtaccgct
    11881 tgtctgtctg caggcgttat cgcttcattc ggcctgaaca caaaacggcg ttcacaactc
    11941 gctttccagt cgataaccgc ctgggtcaga tggtcgccaa accctttcac ttgcttaacg
    12001 ccacgacggg taacatctgc ggctgtttca ataccaaaag accgtaacgc cgctttacgc
    12061 gcagggccaa cgccgggaat agaggcaaca tcaataaaaa atccctccag aaacttctgc
    12121 ttctgccgtt cccttgcagt gtcatgaagt gctgccagag cgcgtttctc ttcttccggt
    12181 aacccgagaa tttcgtcctt cattttttcg agcatcgtcc gtttggcgat aaaaccttcc
    12241 aggccgccta cctgctggat ctggcggact aaatggtcat agtccatttt cgcacgatta
    12301 aatgcctcac ggcgttgctg aacttctgct ttgtacgctt tgcttgtcag actgccaata
    12361 atccagatag ccgccagcac aggaaccaaa ataatataac gcggttctgc ctgaaggccg
    12421 caaagcagca gcgataatgc tgacagtgcg atctcaagca gaatgatgta ttcgcgccgt
    12481 aacaggccta aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa tggcagttgc
    12541 aatgctggcg gtgctactga cgccatcacc atcgcccaga cttttgccag cacaaaatcc
    12601 ccgccggtgg taatgacctc ttcgccgaga tcaataaaat agataacacc ttgattatcc
    12661 agcgcacacc atgggcagtc agccaaatga ccgggataaa catgcattgc cgaaacggta
    12721 catttcttta actgttggcg cagagaatca agtgccgcta cccacgcttt agccgtcgga
    12781 cgcccggttg ccacgccact ttccgtgaat gcctgctgaa acatggcttc aacatcactc
    12841 ggtaacatcg atagcggaat cgatcgtggc ggcggtttta aaccacgtcg ctgattatct
    12901 gacgcgtagg cataacggaa atgggtaata tccgtctcca gcgcattacc cgcatcagag
    12961 ataagcggca caccagaata aggatgccgg ccaccaaaca agacgtgaaa aatcagcaac
    13021 gcaaggccaa aattatcgtg attttttgtg cgttcaaagc cgacaaatga cgacaaggtt
    13081 tgtagctctg gcggcgtaaa atgcgacacg ccgacttcgc ataaatgcag tgtgccattg
    13141 gcgttaatct gaaaggagtc gctatcaatc agtaccactt tgctgtcgcg acctaccata
    13201 aaactgttct ggtttacgtc acccacgacg tgcccgtgct cgtgaaccgt agcaaaagat
    13261 gaagcaatat tgcgcgcaac atagagtaga aaatcccacg cacaatgagg gtaacgttga
    13321 cgacgatgtg ccgggctata gatcatatga atcggttctt taccagaaac ttttggcatc
    13381 ataaaaccga taacttttcc gcctcgtcca ccgtgaagcg ttgcctgcgg ccaggcgaca
    13441 taattcaaca attgcgcgtc agctgtcgca gccataaagg caagtttgtc ctgttttaag
    13501 gcgggtggcg gcgtgtgata aatcttggcg acgctatcga caaactcatt gatatcataa
    13561 accgcgcctt caccgccttt gcccagttca cggcctggcg taacgcactc accagtagca
    13621 gtatataaag tgggtttcat ggtaaccaca gcgccagtgc caacgtttta tcatcatccg
    13681 tacgctcgtt taccgccggg ctggacaaaa actgctttaa caattcaggt aataaatcga
    13741 gttgttcctg cgttgctgac gccagtccat tgaaaaacgg ggtaaagaaa ggtacatgag
    13801 gagaattatc cagcatattt aacgccagtc gctggatacc atccgtaaat gctgcaactt
    13861 tatgcacacg ctcagtgctg gtgaaagttt ccagtctgga aacggcatct tcatcggtaa
    13921 tgaagtgcgt catattggca tattcaccca ccatcggcgt aagcggcaat tgcagaccgt
    13981 gaccaaaatc caccaccacg ccgccatcgc caatctgcat aatcaaggtg ccattagccg
    14041 atgaaatcaa tcccagaaac gtgcaggcaa aatcgcgaac tgctaattct ttggcttccg
    14101 cctcggcaaa aagtcgctga cgaatagtca gcacaatatc cgtggcgagg atatcattaa
    14161 gccccaattc cccaccctgc actttttgcg acatataagc catcgcttca ttgacggcga
    14221 gcatcgcgcc ttcgccaccc tgcgagacgc tgcctgcacc atcagcaaga aacatcacca
    14281 gtaatggttg ctgatcgttt aaccaggcaa cctgcatctg gcaggcatcc tgacaaggta
    14341 aatcagcgct aatgtgcgac gttccaacag cggatgcata aaccagacgc cagctcacac
    14401 tgacgtccag ccttttggcg cttccagcac aacttccgtt cccggcgtgg agcgggaaac
    14461 cgaacgcagc gaactggata accagctaaa cagttcacgg aattgtaatc cttgcagagg
    14521 caaaggctga cgaacgctga tttgtgccag cgtcttcata tccgccccct gaacaccgat
    14581 ggaaaagaaa gcaaaccttt tatcttcttc cccccgaaac actttgttgg ctgcagcctg
    14641 ccactcatcg gttggtgcac catcggtaat caggaaaatc cacgggcgat aatacgaaat
    14701 accatttgca cgatactcac gtttccgctc ctctaccata tccagggctt tggtaatggc
    14761 cgcacccatt ggtgtatcgc cctgggcaaa caggatgggc ggaaaaaaat tagcggcgct
    14821 ggtaaagggt tgttccacat gcaccgggcc gaacgtcaca atgccgagtt ccactctttt
    14881 taaagccagc ggatcagcaa gcagttcatc acgaaaggta accaatccgg cgttaagttc
    14941 attaataggt ctaccgttca tagaaccaga aacatccagc agcaaaatgc aggggcaacg
    15001 tggttccggg ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc tcataagaaa
    15061 tcctttctta gagaattatc gaggtccggt atcataccga ctgcaataat gaaataagag
    15121 caaacgaaga aactttcaga attaggcccg gcggcgcaga aaattttgct attcgcctcg
    15181 caagaacgga gagccgtcga ttgacactaa gggcggagtg acataatttc aggagtgagg
    15241 gttagggaga ggtttccccc tccccctggt gttcttagta agcctggaag ctaatcacta
    15301 agagtatcac cagtatgatg acgtgcttca tcataaccct ttccttatta aaagccctct
    15361 tctccgggag aggctttccc gtttcagcgt cccgctgaaa tcatcggctt acctcctttc
    15421 gccctgaatg cagtctatcg ctaacgcgtg aataaaatag tttcctctgt gattattgcg
    15481 taacgactcg caaagaatat ttgctggcag gatcgcagac t
//
LOCUS       DAPGWD010000097        15515 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPGWD010000097 DAPGWD010000000
VERSION     DAPGWD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.098"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            65..631
                     /locus_tag="RHX88_002967"
     CDS             65..631
                     /locus_tag="RHX88_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV2231634.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            635..1210
                     /locus_tag="RHX88_002968"
     CDS             635..1210
                     /locus_tag="RHX88_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDV2231635.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1252..2304)
                     /locus_tag="RHX88_002969"
     CDS             complement(1252..2304)
                     /locus_tag="RHX88_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDV2231636.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2529..3449
                     /gene="lpxL"
                     /locus_tag="RHX88_002970"
     CDS             2529..3449
                     /gene="lpxL"
                     /locus_tag="RHX88_002970"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDV2231637.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3621..4847
                     /gene="mdtG"
                     /locus_tag="RHX88_002971"
     CDS             3621..4847
                     /gene="mdtG"
                     /locus_tag="RHX88_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDV2231638.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4930..5304
                     /locus_tag="RHX88_002972"
     CDS             4930..5304
                     /locus_tag="RHX88_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV2231639.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5305..5532)
                     /locus_tag="RHX88_002973"
     CDS             complement(5305..5532)
                     /locus_tag="RHX88_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV2231640.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(5705..8248)
                     /gene="mdoH"
                     /locus_tag="RHX88_002974"
     CDS             complement(5705..8248)
                     /gene="mdoH"
                     /locus_tag="RHX88_002974"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDV2231641.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8241..9776)
                     /gene="mdoG"
                     /locus_tag="RHX88_002975"
     CDS             complement(8241..9776)
                     /gene="mdoG"
                     /locus_tag="RHX88_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDV2231642.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYETPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10170..11327
                     /gene="mdoC"
                     /locus_tag="RHX88_002976"
     CDS             10170..11327
                     /gene="mdoC"
                     /locus_tag="RHX88_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDV2231643.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11335..12816)
                     /gene="clsC"
                     /locus_tag="RHX88_002977"
     CDS             complement(11335..12816)
                     /gene="clsC"
                     /locus_tag="RHX88_002977"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDV2231644.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDVMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12758..13291)
                     /gene="ymdB"
                     /locus_tag="RHX88_002978"
     CDS             complement(12758..13291)
                     /gene="ymdB"
                     /locus_tag="RHX88_002978"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDV2231645.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRSAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13386..13696)
                     /locus_tag="RHX88_002979"
                     /pseudo
     CDS             complement(13386..13696)
                     /locus_tag="RHX88_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(13817..14149)
                     /gene="csgC"
                     /locus_tag="RHX88_002980"
     CDS             complement(13817..14149)
                     /gene="csgC"
                     /locus_tag="RHX88_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDV2231646.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14208..14663)
                     /gene="csgA"
                     /locus_tag="RHX88_002981"
     CDS             complement(14208..14663)
                     /gene="csgA"
                     /locus_tag="RHX88_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDV2231647.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14704..15159)
                     /gene="csgB"
                     /locus_tag="RHX88_002982"
     CDS             complement(14704..15159)
                     /gene="csgB"
                     /locus_tag="RHX88_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDV2231648.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3854 a         3985 c         3688 g         3988 t
ORIGIN      
        1 gtgttgcaat tcattctccg acacgccatt ttctttctct cgcaaattcg tattggatgc
       61 acttatgtca ttcacaaata cccctgaacg ctatggtgtt atatctgcgg ccttccattg
      121 gctaagcgca attattgtct acggcatgtt tgctttaggt ctgtggatgg tcacgctcag
      181 ttattacgat ggctggtatc acaaagcacc cgaactgcat aaaagcatcg gtattttgtt
      241 aatgatgggg ctggttattc gcgttctgtg gcgtgtcata tctccaccgc cgggtccgct
      301 gccgagttat tcgcccatga ctcgtcttgc cgcaaaggct ggacatctcg cattgtacct
      361 attgctgttc gccatcggca tcagcggcta tctgatctca actgccgatg gtaaaccgat
      421 cagcgttttt ggctggtttg acgtccccgc gacccttgcc gacgctggcg cacaggcaga
      481 ctttgccggt gccctgcatt tttggctcgc gtggagcgtc gtagtactgt ccgttatgca
      541 cggatttatg gccctgaagc atcatttcat cgataaagac gacactctga agcgcatgct
      601 gggaaagtcg tcatctgact atggagtata aaaaatgaaa aaaagcctgc ttggtttaac
      661 cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc gccgattaca aaattgacaa
      721 agaaggtcag cacgcctttg ttaatttccg catccagcac cttggctata gctggttata
      781 cggcaccttt aaagatttcg acggtacttt tacctttgac gaaaaaaatc cggctgccga
      841 taaagtgaat gtgacaatta acaccaccag cgtcgatact aatcacgccg aacgcgataa
      901 acatcttcgc agtgcagatt tcctcaatac cgcaaaatat ccacaggcaa cattcacctc
      961 caccagcgtg aagaaagacg gtgacgaact ggatattacc ggcgatctga cgctgaatgg
     1021 cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt cagggcgacg acccatgggg
     1081 tggtaaacgt gcaggcttcg aggccgaagg caaaattaag ctcaaggact tcaatatcaa
     1141 gacagattta ggtccagctt ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca
     1201 gcagaagtaa gagtgaaatg ttgccggatg gcacgcatcc ggcaatgata tttattccgt
     1261 tggatcagga atgcccagtg ttgtattcag acgtccacga gacttattaa agatcttatt
     1321 gccattttca cgtcctgccc gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc
     1381 gcagcaaatc tcactacaac aacctttgta tttttccgca catactggac actgaataaa
     1441 cagcaggtgg cagccatcat ttttacagtt ggtatggctg tcgcacggcg cgccgcactg
     1501 gtggcaatgc gcgataatct catccgatat acgttcgccc atccgctcgt caaaaacaaa
     1561 atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc gccttacggg cgtattcaat
     1621 aattccgccc tcgatatgcc agactttgtt gaatccgtta tgtttcatcc aggcactggc
     1681 cttttcacaa cgaatgccgc cggtgcagta catgacgatt tttttatctt tatgtgcctg
     1741 catcatctcg actgcttttg gcagctgctc acggaaggta tctgccggaa tttcgagcgc
     1801 gttttcaaag tgccccactt catactcata gtggttacgc atgtcgataa atagtgcatc
     1861 gggatcgtca agcatggcgt tcacttccgc cgcttgcaga tactcaccaa cattgctggc
     1921 atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga tcgcgtacct tcatgcgcag
     1981 tacccagaag gatttcccgt catcatccaa cgcgatattc aggcgtaaac cctctaaagc
     2041 cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca ttgctcgcag gtacgctgat
     2101 ttgcgcgtta atgccctcat gcgccagata cactcgccca aaaacattca gcgcggtaaa
     2161 cagctgatat aaagcgtcac gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa
     2221 cgaaatggtg gttcgcggtt cgctctcagc caacattttg gcttttagcg cgtcgttgga
     2281 aatgcggttg tgtaacactg gcatggtgta cggttcctgc gagatgggaa agtaaaaatc
     2341 cgcggcatga tatagcaatt atcgataatt aacatccaca cattttacgc tacatttgcg
     2401 cattaaaaat tatttgttat ttacaagcgc ggcaatttcg cccagtcttc agccacaaat
     2461 tttggttgcg ggcgaaaaaa tgcgacaata catacaattg cccgaatagg ttgaaaaaca
     2521 ggattgatat gacgaatcta cccaagttct ccaccgcact gcttcatccg cgttattggt
     2581 taacctggtt gggtattggc gtactttggt tagtcgtgca attgccctac ccggttatct
     2641 accgcctcgg ttgtggatta ggaaaactgg cgttacgttt tatgaaacga cgcgcaaaaa
     2701 ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa
     2761 tggtggtgaa gaatttcgaa tccgttggca tgggcctgat ggaaaccggc atggcgtggt
     2821 tctggccgga ccgccgaatc gcccgctgga cggaagtgat cggcatggaa cacattcgtg
     2881 acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat ccattttctg acactggagc
     2941 tgggtgcgcg gcagtttggt atgcaggaac cggggattgg cgtttaccgc ccgaacgata
     3001 atccactgat tgactggcta caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg
     3061 accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa aggcgaagtg gtctggtacg
     3121 caccggatca tgattacggc ccgcgctcaa gcgttttcgt cccgttattt gccgttgagc
     3181 aggctgcgac cacgaccgga acctggatgc tggcacggat gtccggcgca tgtctggtgc
     3241 ccttcgttcc acgccgtaag ccagatggca aagggtatca attgattatg ctgccgccag
     3301 agtgttctcc gccactggat gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg
     3361 aaaaatgcat catgatggca ccagagcagt atatgtggtt acaccgtcgc tttaaaacac
     3421 gcccggaagg cgttccttca cgctattaaa tctcccatgc cggatgcttc agaatggcat
     3481 ccggcattac cacagcaaat ccccctgatt tagcgataaa agctctctgg attgcgcccc
     3541 ctggaagtcg ggcgcataat tagtgtgctt atcttttctt cttatgttca ccgcgcctgg
     3601 cgcaccaaca gcggattgct atgtcaccct gtgaaaatga cacccctata aactggaaac
     3661 gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg ggccgccttc agtctggtaa
     3721 tgcccttctt acccctctac gttgagcagc ttggcgttac cggtcactcc gccctgaata
     3781 tgtggtccgg tattgtcttc agcattacat ttttattttc ggccatcgcc tcaccgtttt
     3841 ggggtggact cgccgaccgt aaaggccgaa aactcatgct attacgctct gccctcggca
     3901 tgggcatcgt gatggtgttg atgggactgg cacaaaatat ctggcagttt ttgatcctac
     3961 gggcgcttct tgggttactt ggcggatttg tccccaacgc taatgctctt atcgccacac
     4021 aagtaccgcg taataaaagc ggctgggcgc tgggtacgct ctccacaggc ggcgttagtg
     4081 gtgcgttgct cggcccaatg gctggcggcc tgctcgccga tagctacggc ttacgtccgg
     4141 tattctttat taccgccagt gtgctcatac tctgcttttt cgtcaccctg ttctgcatca
     4201 gagaaaaatt ccagccggtc agcaaaaaag agatgctgca catgcgggaa gtggtgacat
     4261 cacttaaaaa cccgaaactg gtactcagcc tgtttgtcac tacgttaatc atccaggtgg
     4321 cgacaggctc aattgccccc attctgacgc tgtatgtccg cgaactggcg ggtaacgtca
     4381 gtaacgtcgc ctttatcagt ggcatgatcg cctcggtgcc aggcgtggcg gctctgctaa
     4441 gcgcaccacg actcggcaaa cttggcgatc gaatcggacc cgaaaagatc ctgattacag
     4501 cgctgatctt ttctgtactg ctgttgatcc caatgtctta cgttcagacg ccattgcaac
     4561 ttgggatttt acgttttttg ctcggtgccg ccgatggtgc actactcccc gccgtacaga
     4621 cactgttggt ttacaactcg agcaaccaga tcgccgggcg tatcttcagc tataaccaat
     4681 cgtttcgtga tattggcaac gttaccggac cattgatggg agcagcgatt tcagcgaact
     4741 acggtttcag agcggtattt ctcgtcaccg ctggcgtagt gttattcaac gcagtctatt
     4801 catggaacag tctacgtcgt cgtcgaatac cccaggtatc gaactgattt ttcgcctttc
     4861 atacttgcaa aagcggagaa tcagctatcc ttttccctga aacctcatca actcaaaggg
     4921 agaatcgtga tgaccatgta cgcaacgctt gaagaagcca ttgacgctgc acgcgaagaa
     4981 tttcttgcag acaaccccgg catcgacgcc gaagatgcga atgtgcaaca gttcaatgcc
     5041 caaaaatacg ttttgcagga cggcgacatc atgtggcaag ttgagttttt tgccgacgaa
     5101 ggggaagaag gtgaatgttt acctatgctt agcggtgaag ccgcgcaaag tgtttttgat
     5161 ggcgactatg atgagataga gatacgccag gagtggcagg aagagaatac attacatgaa
     5221 tgggacgagg gggaatttca gcttgagcca ccgctggata ccgaggaagg acgcgcagca
     5281 gctgatgagt gggatgaacg ttaatcactc atacgggcca tgatgagtgt cgatcggcag
     5341 cagtaaagta tcaaaaacca gagagaatgg cagatcaagg atcgtgacat aacgccaggc
     5401 ggagtcacgc acatcccatt gcacaccggg ataatattgg ttgccatgcc cctgccccgg
     5461 tatagtgcga ctaataatgc tgccacagcc actcagcaag gtcaccatga tgctcaccac
     5521 aattaaacgc atttgatacc tctttcgtaa aaaaataccg gcataacgcc ggtattttta
     5581 ttggctcgtt gtttttgccg gatgcggcgt gaacgcctta tccgacccac aaaatcttgc
     5641 aaaatcaata aattgcagga acaatgtagg cctgataagc gtagcgcatc aggcaactac
     5701 gtttttattg cgaagccgca tccggtttac gcaatgccag tggattgagc tttatccctt
     5761 cgtaataact cacccatgaa gaatatctct ccggggaatt ccagacgcgg aaatgcagac
     5821 gggccatcgt caccggatcg cttagcagca ccaggcgacg atcgcgattc agcttctctg
     5881 gcgtctcgtt cagcgcctgt tcaacgtggc ggtcacgggc gatttccagc accttactgg
     5941 cgcggtgacg cgcggtcgcc attgcggttg ccagagcgtt aaatgacggg ttaaacactg
     6001 cgtgcataaa gccatcatca agggagcgtt gacgattcat ctcaaggaac cgatcggtat
     6061 caaccagcac ctgcggcggc gaatactctt ccgggatcag gaacagtttc cagcgtttgg
     6121 tgcgcagacc aacggtggca cggctggaaa tcaccgaaac aaacggtgac aggatcaacg
     6181 agaagacaat cggtgccagc cagaacagga aacgcagatc caaccacgcc ataccaacag
     6241 cccacactaa ccctaacagc agctgtgagc cgtggcgttt gaacgcttca ccccaggaag
     6301 tggagtcatc atcacgctgc ggtgaattcc acaccacttc ccagccaagg aacgcgctga
     6361 caacgaagac cgtatggaac agcatgcgta ccggagccag cagcacggaa aaaagcactt
     6421 ccagcagcaa cgataatgta acgcgccaga agccgccgta ttctttcgtt cctttgcacc
     6481 agataagcaa aatgctcaat aacttcggca ggaacaacag caccatagtc gaagcaaaaa
     6541 gtgcaatcgc cagctcagga cgccactgcg gccacaccgg gaacaactgc cgtggttgca
     6601 ggaagtattg cggttcggtc aacgcatgta ctacctgcaa tgcagtagag agcgcgagga
     6661 acataaacca cagcggagcg gagagataag acatcacgcc cgtcaggaat accgcacggt
     6721 gaaccgggtg catacccttc accaggaaca gacggaagtt catcaggtta ccgtggcacc
     6781 agcggcggtc acgttttagc tcatcaagca agttaggcgg caattcttca taagaacccg
     6841 ggagatcgta agcaatccag accccccaac ctgcacggcg catcaacgcc gcttccacga
     6901 agtcatgtga caggattgaa ccggcaaagg aaccttcgcc cggcagcgga gccagtgcgc
     6961 agtgctcgat aaacggtttc acgcggataa tcgcgttatg tccccagtag tgcgactcgc
     7021 caagttgcca gaagtgcaaa ccggctgtaa acagtggccc atacacgcgg gtcgcgaact
     7081 gctgacagcg cgcatacagc gtatccatac cggacgcttt cggcgacgac tgaatgatcc
     7141 cggcgttcgg gttggcttcc atcaggcgca ccaggccgca taaacaatca ccggtcatta
     7201 ccgagtcagc atccagcacc accatgtagc tgtactggct gccccagcga cggcagaagt
     7261 catcgatatt accgctttta cgcttcacgc ggcgacggcg gcggcgatag aaaatctgac
     7321 cttcgccacc gacttcagcg ataagctcca tccaggcttt ttgctctgcg acgcagatat
     7381 ccgggttata actgtcacta agaatgtaga catcaaagtg tttggcattc ccggtggctt
     7441 ttactgattc ccacgttgca cgcaggccag caaaaacacg gttcacgtct tcgttacaga
     7501 taggcatgat caacgccgtg cgatgctccg ggtttaatgg ttcatcgcca actgttgacg
     7561 cagatatact gtatttatcg cgaccaataa gcagttgcag gaagcccatt aacgccgtcc
     7621 agaatccggc ggacacccaa cagaacagta ccgcaaagag gatcaggata ccggtttgca
     7681 gcatataagg cagaagctgc ataaaggaaa cccacaaatc ctgaccaacc atatccatag
     7741 gattaatcag cgcccaaccc tgataaggaa gaatggtctt catataccag gtcgcgacga
     7801 cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg gatggtaccg acggtacgcc
     7861 acttttgctc gctctcctgc tcttctttgg tcaaacgagc cagatagcgc ggcgtgacat
     7921 cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt ggtacgccac gggtcgggaa
     7981 acatcgagga gcgttttgct tctggcatcg ccttcagctg atcgcgccct tcgtcgtctt
     8041 taattaactg tccatcagca agtgaatctg gccaggcttg ttccagacgc gcctttaccg
     8101 agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg ttcggcatcc agcgcctgat
     8161 gaacggcgcg gatatcagtc ttcggcaatg ccgctttctc gcttgcggcg atgggcattg
     8221 cgtcaatgta ctcagttgtc ttattcattg gcaggtaact ggtagctcca ggtttcactc
     8281 aacgtctgat cggcattcac cagcgcagca cgcatttcag tggttttctt ggcatctttc
     8341 actttcacac gcatcaccag acgccagcct ttggtaaccg ggttatagcg caccgtgctt
     8401 tcaactatct caccattatc accaatgctg gtttgcgctg tgaccggggt atcctctggc
     8461 agttttttca tctctgcgcc ggtaaaatcg accacaaagg cgatagtacc gtcaggctgg
     8521 cgaatcaggt tcgactgctt cacatccccc gttgaacgac gcgtttgttg cacccatgcg
     8581 ttatctggcg catgcagttt gtcttcatca cggctgaagg tgatggtgta tttaaagttc
     8641 atttctttac ccggctccgg cagctgatcc ggcgtccagt aagcgacgat gttatcgttg
     8701 gtttcatcgt tggttggaat ttccaccagc tcaacgctgc ctttacccca ctcccctttc
     8761 ggagtcaccc atgcgcttgg acgaagatcg taacgatcat cgagatcttc aaagcgggag
     8821 aaatcacgac cgcgctgcaa cagaccgaag ccttgcgggt tctccatcga gaagctgctg
     8881 accgctaaat gtttcgggtt attcaacgga cgccagatcc attcgccatt accggcatgg
     8941 atagacagac cgttagagtc gtgcaactcc ggacgatagt tatttgcagg cgacggttgg
     9001 ttcggcccaa acaggaacat actggttaac ggtgcaaccc ccagtttgcc gactttatcg
     9061 cgcagataga ttttcgactg cacatccaca accgtgtcac gccctggcat aaccacgaat
     9121 ttgtaagcac ctgtcgcgcg cggcgagtca agcaatgcat aaatggttaa acgtttatca
     9181 gtcggttttg gacgctcgat ccagaactct ttgaagcgtg gaaattcttc acccgatggc
     9241 aaggcggtat caattgccag gccgcgggca gaaaggccat aaacctgacc tgcaccaatc
     9301 acgcggaaat agctggcccc gagcatgctg acgatttcat cgtttttatc tttgctgttg
     9361 atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt ttaccgtgtc tttgtcatgc
     9421 tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga ttcgtttgac tgcggtggca
     9481 gtcacttcat ttattttgac cggggtatcg aagtacatac cctgatggta gaactcgagt
     9541 ttgaatgggg tcttcagatt gttccagtac gctttgtcat gattaaactg gatctgctga
     9601 tagtccgcgt atttcatatc gcggaaaacg gagggcaagt tgcttttggg cgtctcgtag
     9661 cctttcccgg ctaaagattg agcttgcttt gcgacatcat caatactgaa agcccagctt
     9721 gaagatgtat acagggttaa cattactgca gcactcaacc aacgcatttt catcatttgt
     9781 agtttatgtt tcataattag taagcacttc cccctttgtg tgcttatatc gatccgatct
     9841 attttaatgg ataaccagcc attccgacaa cagaatcccg gtatcgttca gcacgctggc
     9901 tcatggatta agcacctgat tgcaaccgtt ttcactttcc gtgcttctcc taaagacaga
     9961 agcatcgact tcgtgtaggg tttgccagca attttattta tcaaccgtct tacagacgct
    10021 tccattctgg ttagtggtag cgatgatgct atgcataaaa accttgaagc ctgcgcctgg
    10081 cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg cataacccga cagggataag
    10141 acgaaaaatc cgagattagt taaccatata tgaatccagt acccgcgcaa cgtgaatatt
    10201 tcctcgactc catccgcgcc tggctgatgt tgttagggat cccttttcat atttctttaa
    10261 tctattcgag ccatacatgg catgtgaata gcgccgaacc atcattgtgg ctgacccttt
    10321 ttaatgactt catccactcg ttccgcatgc aggtattttt cgttatatcc ggctactttt
    10381 cctacatgct ttttttacgc tatcccttga aaaaatggtg gaaagtacgt gtcgaacgtg
    10441 taggtatccc gatgttaaca gccatccccc tactgacatt accgcaattt attatgctgc
    10501 aatatgtcaa aggaaaagcg gaaagttggc ctgggctgtc attgtatgac aaatataata
    10561 cgttggcctg ggaattaata tcacacctgt ggtttttact ggtgttagta gtcatgacga
    10621 cgctgtgcgt atggatattt aagcgcatca gaaataattt agaaaattct gataaaacga
    10681 ataaaaaatt ctcgatggta aaactatcgg tgattttttt atgcctcggc atcggttatg
    10741 cggtaataag aagaacgatt tttattgtgt atccgcccat tctgagtaat ggcatgttca
    10801 attttattgt catgcaaacg ctgttttatt tgccgttctt tatcctcggc gcactggctt
    10861 tcattttccc tcatcttaaa gccttgttta ccacgccgtc tcgtggctgt acccttgcag
    10921 cagcattggc gtttgtcgct tatttactca accagcgcta tggcagtggc gatgcctgga
    10981 tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct gtggatggtg aatgtggtct
    11041 tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg ggtgacttat tttgttaacg
    11101 catcgctgtt tatctatctg gttcaccacc cgttaacgct gtttttcggc gcatacatta
    11161 caccgcacat cacctccaac tggcttggtt ttctctgtgg cctgatattt gtagtaggga
    11221 ttgcgataat tctgtatgaa attcatttgc gcatcccgtt actgaagttt ttgttctctg
    11281 gtaaaccggt tgttaagcgt gagaacgata aagcaccagc ccgttaagcc acatttacaa
    11341 taaccattcc acgggcaata tcgacgccag tctgaccata acccgcttcc agaaactggt
    11401 ggcgggttct tttttgagaa taatctcttt ctttgcatga cgatcaaccc agttgatccg
    11461 tccccacctg tccagacgga gctgccaggc cgcatcatac tggctctgaa taaagcgttt
    11521 atcaattaac tgtgccagcg tttcgctctc tatcacgaag cccatttcag tattgagcaa
    11581 tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc gttttaccat cgatgctaaa
    11641 ggttttagca tgcaggctgg ctccggaatt accggttatg ccgcgatcgt gtaacgtact
    11701 actttgttca cgcgtcggct tgagttcata taattccacg ccatagcgga gcaatttttt
    11761 gcgccagcgc gcgtatccgg catggacgac agcaacatcg ttagcggcaa gagaattggt
    11821 taggatcgca atctttaccc cttttctcac catccgtaag agttgcgcca cacctgcgcg
    11881 tgtcggtaca aaataggaag agataatatc gatgcgttca ctgggtgagc ccatgatatc
    11941 gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg ccctcccctt tcgccggatc
    12001 atcacttaat aaacgtgttt tcgcccagat aagcggcaac gttccatcaa ccagatgatt
    12061 tataaacgga ctggattcca ttttgcgtaa gtaacgatgc gtcataacat cgttatgcca
    12121 ggaggcgggt aactcgatgc gatccgccat ttcaccttcc gggacatcca gcacttgctg
    12181 taagggtgaa accgatttgc aataccagta gcgggcgaaa tcatcggcaa cgtcctctac
    12241 cacgggccct attgccataa catctaaatc cgaaaaaagt ggctcttccc ctgccccaaa
    12301 ataggcatca ccaatatttc gcccccctac cagcgtcacc acgccatcga cagtgaaact
    12361 tttattgtgc atacggcgat tgagacggga aaagtcggtg atataaccaa gcggacgcag
    12421 caagcgaaac gagaaaggat taaaaagccg gacttcaatg cggggatggc tgtcaagcaa
    12481 gcgtaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc agcaacaaac ggacgcgaac
    12541 gccacgcttt gctgcggcta acagggcgga aaacagtaac cgacccgaca tatcgtcctg
    12601 ccagatgtaa tactgaacat ccagcgtatg ctctgccatt tcggccagac gataccgggc
    12661 ggcaaacgca tccagacttt tctccagagg gaaaagacca cactgaccgg gatgttgcga
    12721 acacagtggc agcaccgcgc tcgccagccg gggcaaatca ttcatctcct tgttgggtaa
    12781 ggagtctttc gtagaggtgg gcgttttctt catcataaca gacaaagtat acctgttcgg
    12841 gtaaagcgtg acgggtaata aattctgaaa cggttttaac tgcgatttca gccgctgccg
    12901 cacgagggta accataaacc ccagtactga ttgcaggaaa agccactgac gtatagctgt
    12961 ttgccgccac cagtcgtagg ctattgagat aggcatcctg caaaagctgg tcttcgtttt
    13021 gttcaccacc acgccagact ggcccgacgg tgtgcactac ggctttagcg ggaagagcgc
    13081 ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc ctgctgttgc ctgactttta
    13141 aacaggcatc cagcagggcc ggacccgctg agcgatgaat ggccccatcg acgccgccgc
    13201 ctcccattaa tgacggatta gccgcattca caatcacatc aacggccagt ttggtaatat
    13261 caccctgcac aacatgaata cgcgttttca taacgcctcc ttacaccttc ttgtttctta
    13321 agtgtacagc aggctttttg gttaaatcgc atttttctgc tcagcaacgt gcgttgttca
    13381 gaaacttagt tatattcgat gttcattaca gccagctttt tctgtttatc aagatactgc
    13441 acttttactg aggctatttg cgcgttttta acattaccgc tcattaacgc tttgctggaa
    13501 taggttttgc ccggaacaga accattctgt gggcagttca tttctacggg tgatgactgg
    13561 gtgctgacgt cacatgccgg ttccacaatt tggccataaa aatggatccc cccccttgcg
    13621 cggtgcttcc tgcgatggca ataaaaggaa aaagcaaact gccgagcata agagagttta
    13681 aaaaaggtct gaacatttat tctatttcct caatgattag tcatccttga gggttgtgtt
    13741 atccatactt tcaggttggc tgattcgcag cagaccattc tctccagatt catcttatgc
    13801 tcgatatttc aacaaattaa gacttttctg aagagggcgg ccattgttgt gataaatgaa
    13861 gtgactgtcc atcagaaaca gtaacaacta ttttcacccg atcttccggg gaaatattta
    13921 aactcaactt cgtcaaagca ataggttgat tagcaggcaa tgagagggtc ttttcttgct
    13981 tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa tatttgtact ctgcacagac
    14041 aagattgagt aagagtgact tcaggaataa tggtatacac atccccttgc tgggtcgtat
    14101 taaaggttat ctggctggaa agtgccgcaa ggagtaataa cgcattcata ttcttctccc
    14161 gaaaaaaaac agggcttgcg ccctgtttct gtaatacaaa tgatgtatta gtactgatga
    14221 gcggtcgcgt tgttaccaaa gccaacctga gtcacgttga cggaggagtt agatgcagtc
    14281 tggtcaactg cagcaccgtt gccaccaccg aactgtttaa cagtcatttc agaatttttg
    14341 ccgttccact gatcaagagt ggcgctgtta ccgaagccac gttgggtcag atcgattgag
    14401 ctgtcatctg agccctgacc tacatctgca ccattaccac cgccatgctg ggtaatagtc
    14461 aagtcagagt tacgggcatc agtttgcaga gcaagtgcag agttaccgcc accgtactgg
    14521 taaatgttca gctctgaatt tgggccgcta ttattaccgc caccaccgtg gttaccgccg
    14581 ccgccgtact gaggaacaac acctgccaga gcgctaccgg agaatacgat tgctgcaatt
    14641 gctgctactt ttaaaagttt catgtaaaac ccccatcgga ttgatttaaa agtcgaatgg
    14701 aaattaacgt tgtgtcacgc gaatagccat ttgcgactgt ctctgcacta caattgccgt
    14761 tttttgagta ccatactgtg taatatttgc tttattacca gaacctttct ggataatcat
    14821 cgcagtatta ccataagcac cttgcgaaat actggcatcg ttggcactgc ccgcctgatc
    14881 aatatatgca aggttataat ctcctgtctg gtcaatcttt gcccggttgc tactaccttc
    14941 ttgcgcaaca accgccaaaa gttttgagcc tccctgccgt aactgagcac tattattcgt
    15001 cccagcttga ccaattatgg ctgcctgatt aaatgaagac ttactcaatt catttaccgc
    15061 gaagttatat tctgaattag ctaaatcata acctgctgcg gctgcaatcc caggcgcacc
    15121 cagtattgtt aacatcataa ataacaattt gtttttcatg ttgtcaccct ggacctggtc
    15181 gtacatttaa gaaattaaat catttcaact tggttgttaa cgcaacctgt attttgttaa
    15241 cgctgcgtta cgatggaaag tatgtctgcg gaaatatttt taataactca cccgcgcgtt
    15301 gtatttttct ttttttgttc ataccaaagt attaaaaccc acgtaaatac gctgttatct
    15361 acgcaaaaaa tattttgttt tcttttaaat ctccgttttc cgctatcaaa aatcaccaga
    15421 cagtcatttt tcttgcccgt cgctgattgc tgcgatatgt cttgcgcaca agccgtgaca
    15481 aacctcgtct ttttgcagta aaaaattgtc cacgg
//
LOCUS       DAPGWD010000098        15440 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPGWD010000098 DAPGWD010000000
VERSION     DAPGWD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.099"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(124..792)
                     /gene="artM"
                     /locus_tag="RHX88_002983"
     CDS             complement(124..792)
                     /gene="artM"
                     /locus_tag="RHX88_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDV2231649.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(792..1508)
                     /gene="artQ"
                     /locus_tag="RHX88_002984"
     CDS             complement(792..1508)
                     /gene="artQ"
                     /locus_tag="RHX88_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDV2231650.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFNINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1515..2246)
                     /gene="artJ"
                     /locus_tag="RHX88_002985"
     CDS             complement(1515..2246)
                     /gene="artJ"
                     /locus_tag="RHX88_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2231651.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2264..2992)
                     /gene="artP"
                     /locus_tag="RHX88_002986"
     CDS             complement(2264..2992)
                     /gene="artP"
                     /locus_tag="RHX88_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDV2231652.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3210..3725)
                     /locus_tag="RHX88_002987"
     CDS             complement(3210..3725)
                     /locus_tag="RHX88_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2231653.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3851..4174
                     /locus_tag="RHX88_002988"
     CDS             3851..4174
                     /locus_tag="RHX88_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDV2231654.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4171..5001
                     /gene="amiD"
                     /locus_tag="RHX88_002989"
     CDS             4171..5001
                     /gene="amiD"
                     /locus_tag="RHX88_002989"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDV2231655.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     RVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4998..6011)
                     /locus_tag="RHX88_002990"
     CDS             complement(4998..6011)
                     /locus_tag="RHX88_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV2231656.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6110..7540)
                     /locus_tag="RHX88_002991"
     CDS             complement(6110..7540)
                     /locus_tag="RHX88_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDV2231657.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7551..8552)
                     /gene="ltaE"
                     /locus_tag="RHX88_002992"
     CDS             complement(7551..8552)
                     /gene="ltaE"
                     /locus_tag="RHX88_002992"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDV2231658.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8589..10307)
                     /gene="poxB"
                     /locus_tag="RHX88_002993"
     CDS             complement(8589..10307)
                     /gene="poxB"
                     /locus_tag="RHX88_002993"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDV2231659.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10440..11408)
                     /gene="hcr"
                     /locus_tag="RHX88_002994"
     CDS             complement(10440..11408)
                     /gene="hcr"
                     /locus_tag="RHX88_002994"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDV2231660.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11420..13072)
                     /gene="hcp"
                     /locus_tag="RHX88_002995"
                     /gene_synonym="priS"
     CDS             complement(11420..13072)
                     /gene="hcp"
                     /locus_tag="RHX88_002995"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDV2231661.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13216..14115)
                     /gene="lysO"
                     /locus_tag="RHX88_002996"
     CDS             complement(13216..14115)
                     /gene="lysO"
                     /locus_tag="RHX88_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDV2231662.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(14610..15305)
                     /gene="aqpZ"
                     /locus_tag="RHX88_002997"
     CDS             complement(14610..15305)
                     /gene="aqpZ"
                     /locus_tag="RHX88_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDV2231663.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         3787 a         4149 c         4055 g         3449 t
ORIGIN      
        1 cactatgtag gccggataag gcgtccacgc cgcatccggc acaggcaccg tgctgatgtc
       61 tgatgcgacg ctggcgcgtc ttatcagacc tacaaacccc cccggcgaat gtacgcagct
      121 acattaattc cgccgttcga atgccagtgc tttgcgctcg atcagacgca tcatcagcgt
      181 cagcaggccg ttgacgacca ggtaaataat ccctgccgca ccgaacacca ttacatcgta
      241 ggtgcgtccg tacaacaact ggctgtatcc catcacttcc atcagcgtaa tggtgtaagc
      301 cagagaggta cttttgaaca ccagcaccac ttcgttggaa taagaagaga gcgagcgttt
      361 aaaggcatac ggcagcagga tcgccagcgt atcttttttg ctcattccca gggcgctaca
      421 ggactgccac tgaccttctg ggatcgcacg aattgcaccg taaaacagct gcgtggtata
      481 cgccgcactg ttcagcgata acgcaatcag cgcacataac cacggttctg acaacaaatg
      541 ccacagtgcc ggatactcct gcaaagtcgg aaactggccc ggcccgtaat aaatcaggaa
      601 gatctgcacc agcagcggcg taccggtaaa cagcgtgata taaccccgca ccagccacac
      661 cagcaccggc gttttcagcg tcaggatgat ggtaaaaatc aatgccagaa tcagtgccac
      721 aatcagcgag gcaacggtta gcgtcaggct ggtgtgtagc cctttcatca gttcgggtaa
      781 atactcaaac attagctggg cctccgctca aaacgtgtcg cgcgcaggtc aatgcgtttg
      841 agaatgtact gactgagcag ggtgatcacc aggtaaatcg ccgccgccac aatgtaccag
      901 gtaaatggtt cctgggtacg agtagcgatg ctttttgttt gcagcattaa atcattcaca
      961 ctaatcaaac tgaccagcgc ggtatctttc agcagcacca gccactggtt accgaggcca
     1021 ggcagcgcat gacgccacat ctgcggcatc accagacgga aaaagatagc cgatttcgac
     1081 agccccagcg cctgaccaga ctcccactga cccaccggca ccgctttcaa cgcgccccga
     1141 agcgtttgcg aggcataggc ggcatacagc agtgacagag cgatgacacc acaaaggaac
     1201 gggctaacgt cgaagttctc aatgtccatc tgcactggga tctgcacgaa cccaagattg
     1261 atgttgaagc catccgaaag cgtcagcagc agctgcgagg agccaaaata gataaacagc
     1321 accaccagaa tttctggcag gccacgcaga atggttacca gcgctgaacc tgcccacgcg
     1381 acaggacgcc attttgccga ctcccatacc gcaaagaaca tcgccagcgc cagcccgaca
     1441 atcaatgcac aaacggcaag gccgacggtc atcccggcgg cgcttgctaa aggaaaaaat
     1501 tcattcatca ggaattactt ctggaaccat ttgttgtaga tggtttcgta agtgccatct
     1561 ttcttcactt tttccagcgc agtgttgagt ttctgctgca gctcagtgtt gccctgacgt
     1621 accgcgatgc cgaggccagt gccgaagtaa tctttatcgg tcactttgtc gcccaccgcc
     1681 gccagtttcg ggttatcttt cagccactca gtgaccactg cggtgtcacc gaagacgccg
     1741 tcgatacgcc cgttttgcag atccagtttt gcgttctggt agctgtcata cggaacggta
     1801 gtgatttccg ggtgcttatc cataatgaat ttctggtgtg tcgtcccgtt ctgaacgccg
     1861 acttttttgc ctttcagctg atcaacactg gtgtatttgc cttgctgacc cacaaacagg
     1921 gcagagttgt catagtacgg ggtggtaaac agtacctgct tttcacgctc cggagtgata
     1981 tccatgcccg ccatcactgc ttctacgcga cggaatttca ggcttgggat caggctgtca
     2041 aacgcctggt tagagaaagt acaggttgca tcaatctctt tacacagtgc ctgtgccagg
     2101 tcgacgtcaa aaccaacgat ctggttgttt gcatcaatcg attcaaacgg aggataggag
     2161 gcttcggtag caaaacgaat ggtttcggca gctgtggcgg aaagactaaa acctgcaatt
     2221 aacgcggcaa tcagaacttt tttcattgtt gttatcccga atcttagtga gagagatagt
     2281 ttttaaatgc ttcggtttgc ggctcggtaa agcagctcgc gtcgccttgt tctacgatat
     2341 gaccattttc catatacacc actcggctgg cggttttacg cgccacttca acttcgtggg
     2401 tgacgatcac ctgggtaata ttcgtttctg ccagctcacg aatgatgctg acgatttgtg
     2461 ccgtaatttc cgggtccagt gcggcggtcg gttcatcgaa cagcagtacc tgcggttcca
     2521 tcatcaacgc acgggcaata gcaacacgct gctgctgacc accagaaaga tgcagcgggt
     2581 aacgatcgct ataaggtttg agacgcagac gttccagcag tttttctgca cgggccagcg
     2641 cctgatcttt actcaacccc agtacacggc agggcgcttc aatcaggttt tgctgcacgg
     2701 tcagatgcgg ccacaggttg tattgctgaa acaccatgcc aacgttacga cgcaaatcgc
     2761 gaatcgcttt gtcagagggt gttttggtga aatcgaaatg gttgcctgca atgttgagcg
     2821 taccggagcg cggcatctca agcagattga gtacacgcag cagcgagctt ttacccgcgc
     2881 cgctggggcc aagtaacacc agcgtttcgc cctgtgggca atccagcgtg atatcgaaca
     2941 gcgcctgatg cgcgccgtag aagcaattaa tgccgtttaa ttgaatactc attgacactc
     3001 gtatactggc agtctgatag ctattgaggt cgaagatagt acctttgaca gaataattat
     3061 gcaatatttc tgctttaaaa gttaaaagca aagcgcatta ttcaataaac atagcacaaa
     3121 ataacggggg cggtggtcgg cgagcataaa tgtcggcatt cctcacgaaa tgccggacaa
     3181 tttacggggt ttattggttg atcaaggcgt tagcgattct cgatggactg acggagcgta
     3241 cccgccgtgg catgaacgct gccgcctaaa tagcgtacat cgtcgatcac ccaacactgg
     3301 ccttcctgaa tcattaacac ttcatcctgc cagctctgat caccctgttt tagatccaca
     3361 cgcagaggaa tatttcgcgc atcacgattc gggattgttg aggcactggc aacacgggcg
     3421 ctctctggca aggttgtgcg actggagaat ggatcgttgg tcagtagttc ccgatggtta
     3481 ttatcccggg aggcatcgct aagcagtgtc gccagtttgt cgctcagata agggcgcaag
     3541 gcggtgatgt cgttgctgcg gtgcaaaatg cggtagtcat aaaattgctg ggccacgtta
     3601 tccgggcctc cttcaacgca aggaccactg cgtgtgccgt tatctttata agctggagtg
     3661 actgtggtgc aggcactgag gagcagtgcg caggggataa gcattgtcaa tttgctgtag
     3721 cgcataatga tttccttata agcgatcgct ctgaaagcgt tctacgataa taatgatatc
     3781 ctttcaataa tagcgtatca gtccgataat gcttttgaga tcgaaggctt aacaaacaag
     3841 gagatcgatc atgcaatttt ctacaacccc aactctggaa ggccagacca tcgttgaata
     3901 ttgcggtgtg gtgaccggcg aagcgatttt aggtgccaat attttccgtg atttctttgc
     3961 tggtatccgc gatatcgttg gcggacgctc cggtgcgtat gaaaaagaac tgcgcaaagc
     4021 gcgggagatc gcctttgagg aacttggctc ccaggcccgg gcgctggggg ccgatgccgt
     4081 cgtcggtatt gatatcgact acgaaacggt cgggcaaaac ggcagtatgc tgatggttag
     4141 cgtcagcggt acggcggtga aaacgcgtcg atgagaagag tcttctggct gatcgctgcc
     4201 gctctgttac tggcagggtg tgcaggcgaa aaaggcattg tcgagaaaga gggatatcag
     4261 cttgataccc gacgccaggc gcaggcggcg tatccgcgca ttagagtgct ggtgatccac
     4321 tacaccgcag atgattttga tagctcgctg gcgacactga ccgataagca ggtcagctcg
     4381 cattatctgg tccctgcggt accaccgcga tacaacggta aaccgcgcat ctggcaactg
     4441 gtgccggaac aagaactggc ctggcatgcg gggattagcg cctggcgcgg ggcaacgcgc
     4501 cttaacgaca cctctattgg cattgagctg gaaaaccgtg gctggcaaaa atcggccgga
     4561 gtgaaatatt ttgccccgtt tgaaccggca cagattcagg cgcttattcc actggcgaaa
     4621 gatattattg cccgttatca catcaagccg gaaaacgtag tggcacatgc ggatatcgca
     4681 ccgcagcgca aagacgatcc ggggccatta tttccctggc agcaactggc gcagcagggg
     4741 attggtgcct ggccggatgc gcagcgggtt aacttttacc ttgccgggcg cgcgccgcac
     4801 acaccggtag ataccgcgtc attgctggag cttttggcgc gctacggtta tgacgttaaa
     4861 cctgatatga caccgcgcga gcagcggcgc gtgattatgg cattccagat gcatttccgc
     4921 ccgacgttat ataacggcga agcggatgca gaaactcagg cgattgccga agcattgctg
     4981 gagaaatacg ggcaggatta gcgcggcagt tttccgtggt cgcgtagcca ggcggcagtt
     5041 ttctcgatac cttcatccag ggtgatgacc ggctgataac ctaactcttc ctgcgcacgc
     5101 gtaatatcca gcgtaaagtc aaaattcaac ttggagacgc cgtagtgggt cagcggcggc
     5161 tcttttgctg acttgcggcc taaacgctcc atgctgcggg cgatcatatc cagcatcggg
     5221 taggggacgg aacgaatacg acagtcaata ttcaactcgt cgatcagctt ctgcacgatg
     5281 ctgcgcagtg tgcgatgctc gccgttggtg atgttgtaca cacgcccgga aggtagctta
     5341 tcgcaggctt cctggcttgc cagccacatt gcgtgcacgg cattttcata gtaggtcata
     5401 tcgaccagcg cactgccgcc atgcggtaac agaatactgc cgtagtggtg catcatatgc
     5461 gccagacggg gaataaagac tttatcgtgc ggtccgaaca gactttgtgg gcgcagaata
     5521 gtaaagcgcg tttgtggatt cgcctgcgaa agcatattga tcacttcttc gctggctgct
     5581 ttgctgcggg caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt aatatcgcga
     5641 tggtggtgat aatcgaagta cagggagggg gaagagatat gaataaagtt acgcacaccc
     5701 caggcgacag cccattcacc caggcggcga gtggcgcgaa cgttagccag atcgaaagcc
     5761 tgttgtgtcc cccagggtga ggtaaagctg gagcagtgcc acagcgtatc aatgcccgcg
     5821 agcatcactt tagcttgtga tgaaaccagc tcggtcagat ccgccggaac aaactctgcg
     5881 cccatttttt ccagcaattt gcccattgcc tcgttgcgac cggtcgctcg cacgctgatg
     5941 cctttctggc ataaaaactc taccgcgttt cgacctaagc cgctggtggc gccggtaacc
     6001 agtaccttca tatcaatcca ctgttgttga gaaaataacg tgcgcattct tccgtgattt
     6061 cccccatgat gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat taatctgtgc
     6121 tttgttctgc cagtctggcg atttgttttg ccattccgcg aaaaataaac agatgcgcgg
     6181 ggatcatcaa taaccagtaa aacagccccg gcataccgtg cggatgccag aaagcgcgga
     6241 catcgatagt acgatagtcg cctttatctt ccaggctaaa acacagtcgt cccagccccg
     6301 gcgctttcat gccaaataac aacgtaagtt gtttttccgg ttcaacgaca atcactttcc
     6361 agctatctac cgcatcgcca gtctgtaaat attcgcgctc cgggcggcct ttcgccagct
     6421 tatgaccgat cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg ccaaagaaat
     6481 aacgctcttt accgccgatt tggttcacta cctgccataa agcagcaagg ctggcggacg
     6541 ttttaacggt aaaccccgcc tgtttggcaa aataaccgta ctccggtcgc cagcgggcaa
     6601 aggcctgagc gtcgtagccc cagtcgctgg agttgaccag tttttcctcc tctttcaacg
     6661 tgctacgtac cgcgtcatcg aaagcgatca gccgttgtgg gatgagtgca cgtagcgcgg
     6721 tatcatccgc cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg gtggtgggcg
     6781 gtacggaagt aatcacattg agaaaccaca ccgaaatcca gcgggtgggg agggggatgg
     6841 ggatcaacca gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac
     6901 tgagcacctc tggtccggcg gcttcgaaga tgcggtgttc gctggccgga tgatctaaca
     6961 acgccaccag atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt gaacgtaccc
     7021 agcgtggcgg cgttaacact ggcaggttgt agaccatatc gcgcatgact tcgaacgccg
     7081 ctgaacctgc gccaacgata attccggccc gaagttcggt cacaggtaca ttcgcttcac
     7141 gaagaatgtc cgccgtagcc tgacgagcac gcagatgatc cgactgctca tgtggcgggg
     7201 cctgcaacga actgagaaag attaattgct taactggtac ttcacgtagc gcatcgcgga
     7261 cgttgagagc cacctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctgtgca
     7321 ccagaaaata gacggtatcg atatcctgca acagggccgg aaggttatcc ggccagctga
     7381 gatcgacttt atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac
     7441 gtgccgccgc caggatctga tgcccttgct ggctgagtgt gcgcaccaga tgctgaccaa
     7501 tgtagccact ggcaccgaga actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc
     7561 aggaatgcgc gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac gtcaagatgc
     7621 gtcaccaggc ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt catgtattcg
     7681 cctaacgcgg cagcattttc ttccccgacg cgaacaaaca gcatattagt gtcctgacgc
     7741 atcacatccg cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt gtcgtgatct
     7801 tcctgcaacc gtgcgacgtt atttttcagc gcatacatcc cggcggcagc cagaatcccg
     7861 gactgacgca tcccgccacc ggccattttc cgccagcgga tggcacgttt aatgtaatca
     7921 cgattaccga cgagtaatga accgactggc gtcccaagac ctttcgacag gcaaatggtg
     7981 aacgaatcac aatattgcgt gatctctttc agttcgcagc cgtaagccac cacggcatta
     8041 aagatgcgcg caccgtcaac atgcagcgcc agattgcgct cgcgggtaaa ttcccatgct
     8101 tctttcaggt attcacgcgg cagcactttg ccgttgtggg tgttttccag actgagtaat
     8161 ttggtgcggg cgaaatggat atcgtcgggt ttgattttca tcgccacttt atccagcggt
     8221 agcgtgccgt cggcagccgc gtcgatgggt tgcggctgaa tactgcccag caccgctgcg
     8281 ccaccggctt caaacagata gttatgcgcg gcctgaccga caatatactc ttcgccacgt
     8341 tcacagtgac tgagcagagc gaccaggttg gcctgagtgc cggtaggcag aaaaatggcg
     8401 gcttctttac cggaaagctc tgctgcatag tcctgcagag cattaacggt agggtcgtct
     8461 ccgtaaacgt cgtccccaac cggggcggcc atcatcgctt cgagcatggc gcggctcggt
     8521 cgggtaacgg tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc
     8581 cacccttttt accttagcca gttcgttttc gccagttcga tcacttcatc gccgcgtccg
     8641 ctgataattg cgcgcaacat atacaggcta aaacctttgg cctgttcgag tttgatctgc
     8701 ggtggaatgg ctaactcttc tttggcgacc accacatcca ccaacaccgg accgtcgata
     8761 gagaaggcgc gttgcagggc ttcatcaact tcagacgctt tttctacacg gatacccgta
     8821 atgccgcacg cttcggcaat gcgggcaaag tttgtgtcgt gtagttcggt gccgtcagtc
     8881 aaatagccac cagctttcat ctccatcgcc acaaagccca gcacgctgtt gttaaagacg
     8941 acaattttca ctggcagttt catctgcact actgagagga aatcgcccat caacatgcta
     9001 aaaccgccat cgccgcacat ggcgaccacc tgacgttctg gctctgtcgc ctgcgcaccc
     9061 agcgcctgcg gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc
     9121 ttgccgttca tttttagata acgtgccgcc cacaccgttg gcgtaccaac gtcacaggtg
     9181 aaaatagcgt catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg
     9241 gctttctcgc tcggtttagc taaatcgtcc agccctttgc gggcgtcgcg gtaatcttcc
     9301 agcgctttat ccagaaactt gcgatcggct ttttcttcca ccaatggaag caatgcacgc
     9361 agagtcgact tgatatcgcc gaccagtgcc atatccacct tgctgtgagc gccgatgctg
     9421 gctgggttga tatcaatctg aatgattttg gcatcggtcg ggtagaaggc gcggtaggga
     9481 aattgcgtgc cgagtagcac taacgtgtcg gcgttcatca tggtatggaa acctgacgag
     9541 aagccgatta acccggtcat tccaacatca tacggattat cgtattcgac atgttcttta
     9601 ccgcgcaggg catgaacaat aggcgcttta attttcccgg caaactcaac taactcttta
     9661 tgcgcccccg cgcagccgct gccacacatc agggcgatat tgctggaata acgcagcagt
     9721 tgcgccagtt tgcgtaactc ttcttcttcc ggcgtcacga ctggttgtgg cgcatgatac
     9781 cagtgcgtgg ttgccccttc tggcgcaggt tttaacgcca cgtcgcctgg taacacgaca
     9841 accgaaacgc cacggttaag caccgctttg cgcatggcaa tcgccagtac ttgtgggatc
     9901 tgctccgggc tggaaaccag ctcgcaatag tgactacatt cgcggaatag ctcttgtggg
     9961 tgggtttcct ggaaatagcc gctgccaatt tcgctggagg gaatatgagc ggcaatcgcc
    10021 agtaccggaa cgtgattgcg gtggcaatcg aacaggccgt tgattaagtg caggttgccg
    10081 gggccgcacg atccggcgca gaccgccagt tctccgctaa gttgtgcttc agcgccagcg
    10141 gcaaaggccg ccacttcttc gtggcgggtg gacatccact cgatggtgcc catgcgatta
    10201 agactgtcac taagaccgtt cagagagtcg ccagtgactc cccagatgcg tttcacccct
    10261 gccgattcga gtgttttggc gatataagct gcaaccgttt gtttcatggt tctccatctc
    10321 ctgaatgtga taacggtaac aagtttagtt catctgacgg agggggaagg gatgggagag
    10381 aaaggaggca ctaacggtta aatagcccga tgaaaggaat gtcatcgggc ataaggcgat
    10441 tatgcgagaa ccaaatcccc ctgcggatgg caggagcagg ccagtacgta accttcagcg
    10501 atttcggcgt cggtcagcgt cattgtactg ctcaccgtat attcaccgga aaccactttt
    10561 gtcttacagc agccgcaaac acccgcacgg caggcagcga caaccggaac gttattgctt
    10621 tccagcgcct ccagtagcgt ggtgccaacc ggggcgtaaa attctcgtgc cggttgcagt
    10681 ttggtgaatt tcagaccgct ggtcgccgct tctgctactg gggtgaagaa tttctcttta
    10741 aagaaacgcg tcacgccgag cgctttcact tcctgctcta cccaatccat atacggagcc
    10801 gggccgcagg tcatcacggt acgtgaagct aagtcaggta cacctgccag cagttcgcga
    10861 gtgagacgac cagcgataaa gccttcggta acgttatttt ctgccaccag cgttaccgga
    10921 tagttacgcc actcatcggc gaaaataaca tcctgcggcg tacgcacgtt gtagatcacc
    10981 cgcacatcgg cctgtggacg gttcttcgca agccagcgac gcatcgacat aatcggcgtg
    11041 acgccgcagc ctgccgccag caacaggaat ttatcttctg ctttatcgtc gcaggtaaat
    11101 tcccccatcg cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg cgtcagccac
    11161 tgggagccga caccgtcatc aatccgccgc acggtcaggg tgatatattc actcacgcct
    11221 ggcgtggagg aaatggtgta agcacgcagc gtttccgctg agtttcgcac gctgaccagt
    11281 gcatattgcc cggcgcgata tgggtagtaa tcgtggcaaa tcagggaaat cgtccacaca
    11341 tccggcgttt cttgcgtaat gtgatgaacc tgcatccgcc acgggcattg attcgttggc
    11401 atcgtcatcg acaaactcct tacgcgctca acagttgctt catgtcttct tcaacagtgg
    11461 tgatagaacg caggccaaat ttctcgttca gcaccgccag caggtctggt gtcaggaaac
    11521 caggtgcagt cgggccggtg acgatatttt tcacgcccag agaaagcagc gtcagcagaa
    11581 tgacgatcgc tttctgttca aaccaggaga gcaccagcga cagcggcaga tcgttgacac
    11641 cgcagcccag tttctctgcc agagtgacag ccagaataat cgctgagtaa gcatcgttac
    11701 attgacctgc atctaccaga cgcggcagac cttcgatatc gccaaactca agtttgttaa
    11761 agcgatattt accacaggcg agggtcagga tcaggcagtc atccggcacg ctggtggcga
    11821 aatcggtgaa gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc aggaagatat
    11881 gacgcagttt ttcacggcta accagatcaa tcagcgtatc ggctgcacca agcagcgtct
    11941 ggcgaccaaa gcccacggtg atcaggtgag gaatttcgct gtacgggaag cccgccattt
    12001 gttgcgcctg ggcgatgacc gcagagaaat cttcgccatc cagatgacgc acgccaggcc
    12061 agccaacgat gctgcgggtc cagatacgat cgtcataagc gcctacggtt gggtcgatga
    12121 tgcagttcga ggtcatcacg atggggccag ggaaacgagc gaactctact tgctgattct
    12181 gccagccgct gccgtagtta ccgaccagat gcttgaattt acgcagctcc gggtagccat
    12241 gcgcaggcag catttcgccg tgggtgtaga cattaacgcc cgtgccttcg gtctgttcca
    12301 gcaggttgta gagatctttg agatcgtgac cggaaatcag aatgcattta cccgccgtcg
    12361 ctttgacgtt gacctgggtt ggcgtcgggt gaccgtattt accggtttcg cctgcatcca
    12421 gaatgctcat cactttgaag ttcatctggc cgatttccat tgaacactca agaagcgcgt
    12481 tcatatcggc aggccaggtc cccagccacg ccatgatttt atggtactgg gcataaatat
    12541 cgttgtcgta ttgaccgaga acatgcgcgt gttccatata ggccgccgca cctttcaggc
    12601 catacaggca cagcagacgc aggccgagaa tgttttcgcc aatcgccgct ttatctttgt
    12661 taggggtaaa ttctgctgcc tgacgttgca gctcgccgag atcgtcgctc accagttgca
    12721 ggtcagccat cgggttatcg acgcgcgcgt tggcatctac agccaggcat tgcgctttca
    12781 gcgcctcgcg cagggcaatc gcttcacgag cgtagccgac aatacgcgga gaatcgaagt
    12841 taacgttggt cagggttgag aaaaaggcac gtggcgcgaa gctgtcaaca tcgtggttga
    12901 tgatgccgta ttcacgcgct tttaccgccc aggcagaaag cccttgcagc gccgcgatga
    12961 gtaaatcctg aaggtcagaa gtttccgccg ttttaccaca catcccctgc gcgtatgagc
    13021 agccgtttcc tgccggagta cggatagttt gttcacattg cacacaaaac atgatcacac
    13081 cttttaaagt tatatttaat atacatgttt aaggttaaga cgcttaacgc ggggataaaa
    13141 gggatttttc atgcaacttt aagggagatt gatttagcgc aattttggcg gcagggatct
    13201 accgccagag aggtattacg cagagaaaaa ggcgatgagg atcggcacta acaggctaag
    13261 aataaaaccg tgaacaattg ccgccgggac catatccagc ccgccagtac gttgaagaac
    13321 gggcagggtg aaatccattg atgtggcacc gcataagccc agtgcagtag agcggctgcg
    13381 gcgaatcagc ccagggatca acataatagc aatcagttca cgggccagat cattaaaaaa
    13441 cgccgcgctc ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg aaagagaata
    13501 ccagccgaaa ccggaggcca ttgccagcgc ggtattgatg gggagatcaa gaataaaggc
    13561 gttaattaaa ccaccaatta atgaactgac aaccaccacc acggcgacaa tcattccccg
    13621 gcgattaagg acaatctgct ttaaggtcat gccattattg cgcaactgaa taccaacgag
    13681 gaaaagtagc aaaattaacg tgtattcact ggcttcggtc gcgtgttgta agaaagccag
    13741 tccactcaga ccaatggcaa aaccaatcac tactacgccg cacagtttta gcgactccag
    13801 cgccatcgca atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc acggcaggcc
    13861 tcgctccagc cacatcaggg cggcaatatt acacagtaaa ataacggtaa tactgacggc
    13921 agaataatgc agaatcgcca acaggttact ggcgaggtta tcgagaaacg ccagactgat
    13981 acccataaaa aagagaataa ggtaaaccat ccagcttaat agctgattaa taacttttaa
    14041 cgcagcttgt tggcgaagcg gaatgaggta acccacaatc aggggaacca gaatgattaa
    14101 cagcccagaa aacatgaaaa cccagtcctt gcaaagatga agtcgaaatg cgcgatgaca
    14161 cactactgaa agcggaagga cgagtaaagt tgcaattaaa cggaaatgtt atgcataagg
    14221 agcagtagag tattcgtttt catttaaaga tattcttgcg ctttaattac aaactgcacc
    14281 gatgttggtg gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag cccacatgga
    14341 tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgacccgg tagccgtaag
    14401 gtataagtcg agggcaccgc gcagcggcga ttttgttcgc cagagcccgg gggtgcatgg
    14461 ggcggcggcg attggccgcc ccctgcgcgc tccttgcgcc agtggcaata tgtggcttag
    14521 ctcatgaaag gagcgcaaca agatgatgaa tcaacatata acaacatctt aaaaaaaggc
    14581 ctgacattac gccaggcctt ctgcgttaat taatcacgct tttccagcag ggtccggtaa
    14641 atcagaccac cgataatgcc gccgacaatt ggcaccaccc agaagaacca cagttgttct
    14701 aatgcccagc cgccctggaa gatagcaacc gcggtgctgc gcgccgggtt aacagaagtg
    14761 ttagtcaccg gaatactaat taagtgaatc agggttaagg ccagaccaat agcgatcggc
    14821 gcaaaacctg ccggcgcgaa tttgtcggtt gcgccgtgga tcaccaacag gaaacctgca
    14881 ctcaatacca gttcaactac cagcgcggaa agcatggaat aaccgcctgg tgaatgctcg
    14941 ccataaccgt tagaagcaaa accgctggct gccgcgtcaa aacccgtttt accactggca
    15001 attaaataca gcagcgccgc tgcaacaata ccgccgacaa cctgggcaat tacgtagcca
    15061 acgacttctt ttgccggaaa acgtccgcca gcccataaac caatagtgac cgccgggtta
    15121 aaatgaccac cagaaatatg accaacagca aaggccatcg tcagaacggt cagaccgaac
    15181 gccaacgcca cgccggcaaa accaatgcct aattccggga agcctgcggc cagaacagca
    15241 ctaccacagc caccaaaaac aagccagaaa gtaccaaaac attcagctgc taattttctg
    15301 aacatatcca cctcaattaa aaattgaccc tgtgaaaaat atggtcgttt tatagggtcg
    15361 tcgtaaaaaa gtgacgacgg aaataatgcg cggctatttt aaaaacgaag gcgagtcatt
    15421 caccagataa ataaatccag
//
LOCUS       DAPGWD010000099        15438 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPGWD010000099 DAPGWD010000000
VERSION     DAPGWD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.100"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(149..313)
                     /locus_tag="RHX88_002998"
     CDS             complement(149..313)
                     /locus_tag="RHX88_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDV2231664.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            490..1902
                     /locus_tag="RHX88_002999"
     CDS             490..1902
                     /locus_tag="RHX88_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV2231665.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWVELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(2144..3088)
                     /locus_tag="RHX88_003000"
     CDS             complement(2144..3088)
                     /locus_tag="RHX88_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDV2231666.1"
                     /translation="MTNFSTSTPHDALFKSFLTHPGTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            3555..4787
                     /gene="mdtM"
                     /locus_tag="RHX88_003001"
     CDS             3555..4787
                     /gene="mdtM"
                     /locus_tag="RHX88_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDV2231667.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTESRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            4828..6108
                     /locus_tag="RHX88_003002"
     CDS             4828..6108
                     /locus_tag="RHX88_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDV2231668.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPVLLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            6224..7375
                     /locus_tag="RHX88_003003"
     CDS             6224..7375
                     /locus_tag="RHX88_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDV2231669.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            7385..8152
                     /locus_tag="RHX88_003004"
     CDS             7385..8152
                     /locus_tag="RHX88_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231670.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            8149..8406
                     /locus_tag="RHX88_003005"
     CDS             8149..8406
                     /locus_tag="RHX88_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDV2231671.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            8471..9331
                     /locus_tag="RHX88_003006"
     CDS             8471..9331
                     /locus_tag="RHX88_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDV2231672.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            9399..10577
                     /locus_tag="RHX88_003007"
     CDS             9399..10577
                     /locus_tag="RHX88_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2231673.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFSWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(10590..11144)
                     /gene="kptA"
                     /locus_tag="RHX88_003008"
                     /pseudo
     CDS             complement(10590..11144)
                     /gene="kptA"
                     /locus_tag="RHX88_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            11394..12077
                     /locus_tag="RHX88_003009"
     CDS             11394..12077
                     /locus_tag="RHX88_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231674.1"
                     /translation="MTQQGDAVVGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFIGANILRVWLNFEER
                     RNPTQGAQA"
     gene            12074..12535
                     /locus_tag="RHX88_003010"
     CDS             12074..12535
                     /locus_tag="RHX88_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDV2231675.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGAITGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            12548..13720
                     /gene="iadA"
                     /locus_tag="RHX88_003011"
     CDS             12548..13720
                     /gene="iadA"
                     /locus_tag="RHX88_003011"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV2231676.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVVIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            13785..14696
                     /gene="hypT"
                     /locus_tag="RHX88_003012"
                     /gene_synonym="qseD"
     CDS             13785..14696
                     /gene="hypT"
                     /locus_tag="RHX88_003012"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDV2231677.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(14689..15081)
                     /gene="iraD"
                     /locus_tag="RHX88_003013"
     CDS             complement(14689..15081)
                     /gene="iraD"
                     /locus_tag="RHX88_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDV2231678.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(15078..15161)
                     /gene="idlP"
                     /locus_tag="RHX88_003014"
     CDS             complement(15078..15161)
                     /gene="idlP"
                     /locus_tag="RHX88_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDV2231679.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         3484 a         3925 c         3983 g         4046 t
ORIGIN      
        1 gacgcataga tgatgacccc cagagagaca gctcaggtca ttatctgtat gtaaatcaga
       61 aaaatatgtt gtaaacgact tttatggttg gattttgtag caattttgaa tgcagacagg
      121 cagcatttgc caacacgccg cccatagttc actccacatc ctccctgcgt aacccgatat
      181 ccctcaaccg ttcatcactc atctgctgta acacccgtcg ggcctgcatt tgccgccgcc
      241 aacgcctcac cgcctgccag agttgcacaa gtctgataaa cggcgttttg gctttgtttt
      301 cgtgaaattc catctgctgc ctcctcatct ttcagaggct tcatcatccg tcttctggca
      361 gttcacaata cagatgcaaa tattagtttt atttaacata cagacaggtt aaaacagcat
      421 ctgcatccgg ttttttagcc tcatctgtac cggtttttct ctctgtatgg tccaaataag
      481 gaatacagca tgacgcgtta tcaacatctg gcaactctgc ttgccgagcg gattgagcaa
      541 gggctgtatc gtcacgggga gaaattgccg tcggtgcgca gcttaagtca ggagcacggc
      601 gtcagcatca gcaccgtgca acaggcgtat cagacgctgg aaacgatgaa gctcatcact
      661 ccgcagccgc gttcgggtta ttttgtcgca caacgtaaag cccagccgcc tgtaccgccg
      721 atgacgcgtc cggtgcagcg cccggtggaa attacccagt gggatcaggt gctggatatg
      781 ctggtggcgc atagcgacag ttccattgtt ccgttaagca aaagcacgcc ggatgtcgaa
      841 acgcccagcc tgaaaccgct ctggcgggag ctaagccggg tggtgcagca taatctgcaa
      901 accgtgctcg gttatgactt gttagccggt cagcgggtat tgcgagagca gattgcccgc
      961 ctgatgctcg acagcggctc ggtggtcacc gccgatgaca tcatcatcac cagcggctgc
     1021 cataactcga tgtcgctggc gttaatggcg gtgtgtaaac cgggcgatat tgtcgcggtc
     1081 gaatctccct gttattacgg ttcgatgcag atgctgcgag gcatgggcgt gaaagtgatt
     1141 gaaatcccaa ccgatccaga aactggcatc agcgttgaag cactggaact ggcgctggaa
     1201 cagtggccga ttaaaggcat cattctggtg ccaaactgta ataatccgct gggatttatt
     1261 atgccggacg cacgcaaacg ggccgttctc tctctcgctc agcgtcatga tattgtgatt
     1321 tttgaagatg atgtctacgg cgaactggcg acggagtatc cgcgcccgcg gaccattcat
     1381 tcctgggata tcgacgggcg agtgctgttg tgcagctcgt tcagtaaaag cattgcacct
     1441 ggcctgcgcg tgggttgggt cgcaccgggg cgctatcacg ataaactgct gcatatgaaa
     1501 tacgccatca gcagctttaa tgtaccgtcc acgcaaatgg cggcggcaac gtttgtgctg
     1561 gaaggtcact atcatcgcca tatccggcgg atgcggcaga cttatcagcg caatctggcg
     1621 ctttatacct gctggatacg ggaatatttt ccctgcgaaa tctgtattac gcgcccgaaa
     1681 ggcggatttt tactgtgggt agaattgcct gaacaggtcg atatggtctg cgtcgcgcgg
     1741 cagctgtgcc gcatgaatat ccaggtggca gcaggctcga ttttctcggc ttccggcaaa
     1801 taccgtaatt gtctacgcat caactgcgct ttgccgctca gcgaaaccta tcgcgaagca
     1861 ctaaagcaaa ttggcgaggc cgtgtatcgg gcaatggaat aaattcgaat tagtggcgaa
     1921 gcacctgatt tcctctcacg aatcgcctgt aactcgttgt atctttgcag ggtcatcgcc
     1981 acgttccagc atggcttagg caaagtgtgc ttcgttcata ccggatgcgg cgtgaacgcc
     2041 ttatccggcc tacaaaccgc gcaaattcaa tagattgcag aggaaatgta ggtctgataa
     2101 gcgtagcgca tcaggcagct ttgcgtttgt catccgtctc tgattagtat tcgatccatg
     2161 gatcgcgctt gatttcaggc aagggctgct ctaatgcctg catttgctct gtcgaaagtc
     2221 cggtaatctt ttgtatccat tcaggatctg ccccattctg caacaacaat ctaaggaggc
     2281 gttgttcccc ttcttctttc cccttttcca tccccaacag ccatccatca ttataaattc
     2341 gctctgcaat cgtcattatt cgctccctgt gttgtggcat tcgacgggta agttcgctga
     2401 taaacgcctt aaaacgcgct tcatcgccag tcagtaaaat gtaatttaac agcgcagtta
     2461 tctggctgtc attagcacac tctgtaacca gtaatactac cagttggtca ataagcccca
     2521 tcagatcgcg ctggcgaata tgcttttgga tcaactccaa cagggcgact ctgcgatgct
     2581 gcacaatctc gtcgtctggc acgacagtga catccaccag cgggaacgct gcgctataaa
     2641 gtttccgtgc ggtggtcggg gcggcaaatt cgtccagcca gcacagggac caggggtaag
     2701 gactacggct accgtgataa aacagcatcg ggataaccag cggtagcggc cggcgtttat
     2761 catgctcgat atggcgctgc atcaccgcca tggaatatcg catcaggcga aacgccatat
     2821 gaatatcctc gcggctctga tgttcaatca ctacgtaaat ataaccatca ccttcacggg
     2881 tctttaccga ccacagaata tcggagtgta gcgcccgcaa tttttcatcg acaaagctgg
     2941 cggattccag ttttaagctg tcgagatcgc acagttcacg taaatcttta ggtaagtgga
     3001 tctccataaa atcccgcgcg gtgccagggt gcgtgagaaa ggacttaaat aatgcgtcat
     3061 gcggcgtgct ggtcgagaag tttgtcatgg cgatccgtca cctgtagata agatggcccg
     3121 actttagcgg ctaacaaaac cattctcacc cggcaatttc ccgttccccg aggcgctttc
     3181 caggctattt ttattcttct gcaaacgata cattttttcg gaaacaggct cgcgttttac
     3241 atttcctgta cccggcagat attccccttt ccaccgcact cttttgatta accgcaaatt
     3301 ttaattacac ttaaggtgta tattttctat gcaacccatc aattaaagag gtgtaatgtg
     3361 ctgatgacta tttgtaatcg ttatacattc tgacctggag tcagaaggta tttctctgtc
     3421 tgtgtgttca caggcagtgt ggttgattac atgaaattca gtacatttgt ccttctcgcc
     3481 tgcctttcgt cattaccgac ggtattgaat ttcgttttcc ccattggggt tctccggaca
     3541 aggagttgtt tgttatgcca cgtttttttg cccgccatgc cgccacgctg tttttcccga
     3601 tggcgttgat tttgtatgac tttgccgcgt acctgtcgac ggatctgatc cagccgggga
     3661 tcattaatgt ggtccgtgat tttaatgccg atgtcagtct ggccccggca gccgtcagtc
     3721 tctatctcgc gggcggtatg gcgttacagt ggctgctggg gccgctttcc gacaggattg
     3781 gccgcaggcc ggtgctgatt accggggcgc taatatttac ccttgcctgc gctgcgacaa
     3841 tgttcacaac gtctatgaca cagtttctta tcgcgcgtgc aattcagggc accagtatct
     3901 gttttattgc caccgttggt tatgtcacgg tgcaggaggc gttcggacag acaaaaggga
     3961 tcaagttgat ggcgattatc acctccatcg tactgattgc gccgattatt ggcccgcttt
     4021 ccggcgcagc tctgatgcac tttgtgcact ggaaagtcct ttttgccatc attgcagtta
     4081 tgggttttat ctcatttgtt ggcttactgc tggcgatgcc agagacggtg aagcgcggcg
     4141 cggttccatt tagcgccaaa agcgtctttc gcgattttcg taatgtcttt tgcaatcggc
     4201 tgttcctctt tggcgcagca accatctctt taagctatat cccaatgatg agttgggtgg
     4261 ctgtctcgcc ggtgatcctg atcgacgcgg gcggcttaac aacttcgcag ttcgcctgga
     4321 cacaggttcc ggtgttcggc gcggtgattg tcgcgaatgc catcgtggcg cgtttcgtta
     4381 aagatccgac cgaatcgcgc tttatctggc gtgctgtacc cattcaactg gtcggcctcg
     4441 cgctgttgat tatcggcaat ctgctgtcac cgcacgtctg gctgtggtcg gtgctgggca
     4501 ccagtttgta tgctttcggg attggtttga tcttcccgac cttattccgc tttacgcttt
     4561 tttccaataa cttaccgaaa gggaccgtct ccgcatcgct gaatatggtg atcctgatgg
     4621 tgatgtctgt ctcggtcgaa atcggccgct ggctgtggtt taacggcggt cgcttgccgt
     4681 ttcatctgtt agccgtcgtg gcgggcgtta tcgtcgtttt caccctggca ggattgctta
     4741 atcgcgtgcg ccaacatcag gctgccgagc tggcggagga gcggtgattt ttgcgcgatc
     4801 ctgccgtcag gctctattct taacgttatg aataaactca ttgaactcag acgcgccaaa
     4861 atgttggcgc tctctttact gcttatcgcc gctgctacct ttgtcgttac gctgtttttg
     4921 ccgcccaatt tttgggtgag cggcgtgaag gcgattgctg aagcggcgat ggtcggcgcg
     4981 ctggcggact ggtttgcggt ggtggcgctg tttcgccgcg tgccgattcc gatcatttct
     5041 cgtcatacgg cgattatccc gcgtaataaa gaccggattg gcgaaaatct cggccagttc
     5101 gtgcaggaaa aatttctcga tacccagtcg ctggtggcat tgattcgacg tcacgaaccg
     5161 gtgttgctga ttggcaactg gtttagccag ccagaaaacg cccgccgcgt tggtcagcat
     5221 ctgttgcaga tcatgagtgg ttttcttgaa ctgaccgatg atgcgcgtat tcagcgcctg
     5281 cttaagcgcg cggtccatcg ggcgattgat aaggtcgatc tttccggaac cagtgcgttg
     5341 atgctggaga gtatgaccaa aaacgatcgt catcaggtgc tgctcgatac gctgatcgca
     5401 cagttgatcg cccttctcca gcgcgataaa tcgcgcaagt ttattgccca gcaaattgtt
     5461 cgctggctgg agagtgagca tccactgaaa gccaaaattc tccccaccga atggttgggc
     5521 gaacatagcg cggagttggt ttctgacgcg gtgaattctt tacttgatga tatcagccgt
     5581 gatcgtgcgc atcagatccg tcatgcgttt gatcgcgcca cttttgccct gatcgacaag
     5641 ttgaaaaacg atccggaaat ggcagcgcga gccgatgccg taaaaagtta tctgaaagaa
     5701 gatgaagctt ttaaccgcta tctcagtgaa ttgtgggggg atttacggga gtggctgaaa
     5761 gcggatatca acagtgaaga ttctcgtgtg aaagaacgta tcgcgcgggc gggtcaatgg
     5821 tttggcgaaa cgttaattgc cgatgatgcc ttgcgggcgt cgttaaatgg tcacctggaa
     5881 caagccgcgc accgcgtcgc gcctgagttt tccgcattcc tgacgcgcca catcagcgat
     5941 acagtaaaaa gctgggatgc gcgggatatg tcgcggcaaa tcgagttaaa tatcggcaaa
     6001 gatctgcagt ttatccgtgt caacggtacg ctggttggcg gttgtattgg gctaatttta
     6061 tatttgttgt cgcagctccc ggccttgttc cccctcggca atttttagaa atccatgaaa
     6121 aactaagtgg ctaaccgcga gagagatcaa ataaaccaca ctaaaagaat ggtaataatg
     6181 tggcctcttt tcattatctc ccgtggtacg gggaaggaaa atcatgtcac ttatcaccga
     6241 tctacccgcc attttcgatc agttctctga agctcgccag aaaggctttc tcaccgtcat
     6301 ggatctcaag gagcgcggca ttccgctggt tggcacttac tgcaccttta tgccgcaaga
     6361 gatcccgatg gcagccggtg cggttgtggt ttcgctctgt tctacctctg atgaaaccat
     6421 tgaagaagcg gagaaagatc tgccgcgcaa cctctgcccg ctgattaaaa gcagctacgg
     6481 cttcggcaaa accgataaat gcccctactt ctacttttcg gatctggtgg tcggtgaaac
     6541 cacctgcgac ggcaaaaaga aaatgtatga atacatggcg gagtttaagc ccgttcatgt
     6601 gatgcagttg ccgaacagcg ttaaggatga tgcctcgcgt gcgttatgga aagccgagat
     6661 gctgcgcttg caaaaaacgg tagaagaacg ttttgggcac gagattagcg aagatgctct
     6721 gcgcgatgcc attgcgctga aaaaccgcga acgtcgcgca ctggctaatt tttatcatct
     6781 tgggcagtta aatcctccgg cgcttagcgg cagcgacatt ctgaaagtgg tctacggcgc
     6841 aaccttccgg ttcgataaag aggcgttgat caatgaactg gacgcgatga cagcccgcgt
     6901 tcgtcagcag tgggaagaag gccagcggct ggccctgcgt ccacgcattt taatcaccgg
     6961 ctgcccgatt ggcggcgcag cagaaaaagt ggtgcgcgcg attgaagaga atggcggctg
     7021 ggttgtcggt tatgaaaact gcaccggggc gaaagcgacc gagcaatgcg tggcagaaac
     7081 gggcgatgtc tacgacgcac tggcggataa atatctggcg attggctgct cctgtgtttc
     7141 gccgaacgat cagcgcctgc aaatgctcag ccagatggtg gaagaatatc aggtcgatgg
     7201 cgtagttgat gtgattttgc aggcgtgcca tacctacgcg gtggaatcgc tggcgattaa
     7261 acgtcatgtg cgtcagcagc acaacattcc ttatatcgct attgaaacag actactccac
     7321 ctcagatgtc gggcagctca gtacccgtgt cgcggccttt attgagatgc tgtaaggagt
     7381 ggcagtgaca tattcgattg gcattgattc tggttctacc gccaccaaag ggatcttact
     7441 ggcagacggc gtgattacgc gccgtttcct cgttccaaca ccctttcgtc cggcaacagc
     7501 aattactgaa gcctgggaaa ctctgcgcga agggttagag acaacgccgt ttctgacgct
     7561 caccggctat ggacggcaac tggtggattt tgccgataaa caagtgacgg aaatctcctg
     7621 tcatgggctg ggcgcacggt ttcttgcgcc agcaacgcgc gcggtaatcg acatcggtgg
     7681 tcaggacagc aaagtgattc agcttgatga tgacggtaac ctgtgcgatt tcctgatgaa
     7741 tgacaaatgc gcggcgggca ccgggcgttt cctggaggtg atctcgcgca cgcttggcac
     7801 cagcgtcgag caactcgaca gcattaccga aaatgtcacg ccgcacgcca tcacgagtat
     7861 gtgcacagtg tttgccgaat cagaagtgat cagcctgcgc tcagcgggcg tcgcgccaga
     7921 agcgattctc gccggagtga ttaacgcgat ggcgcggagg agtgccaatt tcattgctcg
     7981 tctctcctgt gaagcgccga ttctgtttac tggtggcgtt agtcattgcc agacgtttgc
     8041 ccggatgctg gaatctcatc tgggaatgcc ggtaaatacc catcctgatg cgcaatttgc
     8101 tggcgcaatt ggcgcggcgg taattggtca acgagtgagg acacgccgat gaaagagttt
     8161 ttatttttat ttcactccac ggtcggcgtc atacaaaccc gcaaggcgtt gcaggcagcg
     8221 ggcatgactt ttcgcgtcag cgatattccc cgtgatttac gcggcggctg cgggttatgc
     8281 atttggctga cctgtccccc cggcgaggaa atacaatggg tgatccctgg gcagactgag
     8341 tcaatttatt gccagcagga tggtgtctgg cgctgcattg cacattacgg ggtttctccg
     8401 cgttaaggat tcattaaggt cgctacgtat acttctcgat atcttattga tttgccttaa
     8461 agggattacc gtgacaaaaa gtatttcgtt aagtaaacgc atctccgtta ttgtgattct
     8521 ttttgccatt gtggcagtgt gtactttctt cgtgcaatct tgcgcccgta aaagcaatca
     8581 tgcagcatca tttcagaatt accacgccac cattgacggc aaagaaattg caggcataac
     8641 gaataatatc tcttcgctga cctggtcggc acaaagcaat actcttttta gcactatcaa
     8701 caaacctgcc gccattgttg aaatgaccac caatggtgat tttatccgca caattcctct
     8761 ggattttgtt aaggatctcg agacgatcga atacattggc gataatcagt ttgtcattag
     8821 cgatgagcgc gattacgcca tttatgtgat ttcactgacc ccaaactcgg aagtaaaaat
     8881 cctcaaaaaa ataaaaatcc cgctgcaaga ctccccaacc aattgcggtt ttgaagggtt
     8941 ggcttattct cgccaggatc acactttctg gttttttaaa gagaaaaatc cgattgaggt
     9001 ctacaaagtt aacgggctgt taagtagcaa tgaattacac atcagcaaag acgaggcact
     9061 acaacggcaa tttactttag atgatgtgtc cggcgcagaa tttaaccagc aaaaaaatac
     9121 gcttctggtg ctgtctcatg aatctcgcgc actccaggaa gtaaccctgg ttggcgaggt
     9181 tatcggtgaa atatcactaa ccaaaggtag tcgaggtttg tcgcataaca tcaaacaggc
     9241 cgaagggatt gcgatggatg cctctggtaa tatttatatc gttagcgaac caaatcgttt
     9301 ttatcgcttt acccctaaat catcgcatta acaggctggc atatttcacc tttttgttat
     9361 taacttgtta acaacaaaaa tgacatacaa ggttaaacat gccttcgtcc tcgcatcccg
     9421 tagaacgctt ttctttcagc accgcgcttt tcgggatgct ggttctgacc ctaggtatgg
     9481 gattaggtcg cttcctttat acgcctatgt tgcccgtcat gatggcggaa ggggcgtttt
     9541 catttagcca gctctcgtgg attgccagcg gcaactatgc ggggtatctg gctggcagtc
     9601 tgctattttc gtttggcgca tttcaccagc cgtcgcgcct acgcccattt ctgttggttt
     9661 ctgccctggc gagcgggttg ttgatcctcg caatggcatg gttgccgcca tttattctgg
     9721 tgttattgat tcgcttcctg gcgggtgtcg ccagcgccgg tatgctgatt tttggttcga
     9781 cgctgattat gcagcacacg cgccatcctt ttgtgctggc agctttgttt tctggcgttg
     9841 gcattggcat tgcactgggc aacgaatatg ttctggcagg cctgcatttt gccttatcct
     9901 cgcaaacgtt atggcaaggc gccggggcgc tttctggcat gatgctgatt gcacttacgc
     9961 ttttaatgcc ctcgaaaaaa cacgccatcg caccaatgcc attggcaaaa acggagcaac
    10021 agataatgag ctggtggtta ctggctattc tgtacggcct ggcaggattt ggttatatca
    10081 tcgtcgcgac ttacctgccg ctcatggcga aagatgcagg ctcaccattg ttaaccgccc
    10141 atctctggac gttagtcggc ttatcaattg tgccaggctg ctttagctgg ctatgggcag
    10201 caaaacgttg gggggctttg ccttgcctga cggcgaattt gctggtgcag gctatcagtg
    10261 tgctgcttac tctcgccagc gactctcctc tcttgcttat cattagtagt atcggttttg
    10321 gcggtacctt tatgggcacg acatctctgg tgatgactat cgcccgccaa ctgagcgttc
    10381 cgggaaatct caatctttta ggctttgtga cgctcattta cggcattgga caaatcctcg
    10441 gcccggcgct gaccagtatg cttggcaatg gaacatcggc gcttgccggc gccactctct
    10501 gcggcgcggc ggcgttgttt attgctgcgc ttatcagcac agtacaatta tttaagctgc
    10561 aagtggtcac ttcatgagga gtgtttccct taccactcaa gaaattcgac cgcaaccgtt
    10621 gatgtcagcc agactccgtt ttccgcttgc cagaaaggga tgcctcgttt cgccatttcc
    10681 tgtgctttga cggttaagat aaccggcgat ccgtgtcttg ctcacacttt gcgtgccgtt
    10741 gcttcatcag cagagagatg cacgtaatga cgatcccctg caatcagccc ctgttttttt
    10801 atctcgtcaa ggaaacggct tgccgtaccg tggtagagaa actgtggcgg tgttttttcg
    10861 gcaaaggaaa tagcgacctg cgaagtcgaa tgcccctgaa ccgcgcggat gcacctacca
    10921 tcactggaat aactaaaacg ctttttatcg ctggttgcga ccacggtatc cagcaatgca
    10981 cgggttaacc gcttgcccgc tttttgggca cagagtataa gcttatcgat atccgcccat
    11041 ccttcacggt ccagtacgat accaatcgcc tccggtttgt gtcggaggac aaaacttaaa
    11101 aacttgctcg tatccgccag ctctttttcg ttgtactttg ccatcgcgtt ttcgtccctg
    11161 gattttctgt ttttatttac tgatgtcatt gtcactgggt ttatgaattt tctgcaactc
    11221 aatcctgcgc agagcctttt ccacacagcg taattttacc gctatgcaaa aaacgcatag
    11281 ccagaaacat acttgcattt tccccatacc cacatggctg ataaggtttt tccgctccag
    11341 gaattttcct ggctcaagca acacacaaac acaaaaaaac aatggggatt gttatgacac
    11401 aacaagggga tgctgtagtc ggggagctgg cgacagaaaa agtgggcatt aaagggtatc
    11461 tggctttttt tctcaccata atcttttttt ccggtgtttt ttccggaaca gacagctggt
    11521 ggcgcgtttt tgatttttcc gtgctgaacg gatcgtttgg gcaactgcct ggtgcgaatg
    11581 gagccaccac ctcatttcgc ggggcgggtg gtgcgggggc taaagatggt tttctctttg
    11641 cgctggagct ggcaccgtca gtcattctct ctctggggat tatttcaatt accgatggtc
    11701 ttggtggatt acgcgccgcg cagcagttaa tgacacctgt gctaaaaccg ttgttgggca
    11761 ttccagggat ctgctcgctg gcgctgatcg ctaacttgca aaacactgat gccgccgccg
    11821 gtatgaccaa agagctggcg caggaaggtg aaattaccga acgcgataaa gtgattttcg
    11881 ccgcatatca aaccagcggt agcgccatca tcaccaacta cttctcttct ggcgtcgcgg
    11941 tgtttgcctt tctgggcacg tccgtaatcg tacctttagc cgtcatcctg gtgtttaaat
    12001 ttatcggagc caacattttg cgcgtctggc ttaacttcga agaacgtcgc aacccaacgc
    12061 aaggagcgca agcatgacaa ctcaggtccg caaaaacgtc atggatatgt ttatcgatgg
    12121 cgcgcgccgt ggctttacta tcgccacgac caacctgctg ccaaacgtgg tgatggcgtt
    12181 tgtcatcatt caggcgctga aaattaccgg cctgctcgac tgggtggggc atatctgtga
    12241 acccgtaatg gcgctgtggg gattacccgg tgaagcggca accgtccttt tagccgcatt
    12301 aatgagtatg ggtggcgcag tcggtgttgc cgctagtctg gcaaccgctg gcgcaataac
    12361 cggtcacgat gtcaccgttt tactgcccgc gatgtattta atgggcaacc cggtgcaaaa
    12421 tgtcggtcgc tgcctgggga cagccgaagt gaatgccaaa tattacccgc atatcattac
    12481 ggtctgcgtg attaacgcat tgctgtcgat ctgggttatg cagcttattg tttaacaagg
    12541 agttaccatg attgattata ccgcagccgg ttttaccctg ctgcagggag cgcatttgta
    12601 tgcgccggaa gatcggggaa tttgcgatgt cctcgtcgct aacggcaaaa ttatcgccgt
    12661 tgccagcaat atcccttctg acattgtacc ggactgcacg gttgtcgatc tcagtgggca
    12721 gatcctctgc ccaggtttta ttgatcaaca cgtccatttg attggcggtg gcggcgaagc
    12781 aggtcccacg acgcgcacgc cggaagtggc gctaagtcgc ctgacggaag cgggcgtcac
    12841 gtcagtggtt ggtctgctgg gcaccgactc tatctctcgc cacccggaat ccctgctcgc
    12901 caagacccgt gcgctcaatg aagaaggcat cagcgcctgg atgctgaccg gcgcttatca
    12961 tgtcccttcc cgcactatta cgggttccgt ggaaaaagac gtggtgatta tcgatcgtgt
    13021 gattggcgtg aaatgcgcca tatctgatca ccgttctgcc gcaccggacg tttatcacct
    13081 ggctaatatg gcggcggaat cccgcgttgg cggtttgctc ggcggtaaac ctggcgtcac
    13141 cgtgttccac atgggcgaca gcaaaaaggc gttacagcct gtctatgacc tgctggaaaa
    13201 ctgcgatgtg ccaatcagca agttgctgcc gacccacgtt aaccgcaacg ttccgttgtt
    13261 tgagcaggcg ctggagttcg cgcgcaaagg cggcaccatc gatatcacca gcagcattga
    13321 cgaaccggtc gcccctgccg aaggtattgc ccgcgccgtt caggcgggta ttccgctggc
    13381 acgcgtcacc ctcagctccg acggcaacgg tagccagccg ttcttcgatg acgaagggaa
    13441 tttaacccat atcggtgttg ccggttttga aacgttgctg gaaaccgtgc aggtgctggt
    13501 caaagactat gatttcagta tcagcgatgc cctgcgcccg ctcaccagta gcgtagccgg
    13561 tttccttaac ctgaccggga aaggcgaaat tctgccaggc aatgacgcag acttgctggt
    13621 catgacgcca gaattgcgca ttgagcaggt atacgctcgc ggcaaactga tggtcaaaga
    13681 cggcaaagcc tgcgtgaaag gaacgtttga aacggcataa acggttgtca gccgcccgaa
    13741 tggacgtaga atgcccgcaa gggcggctga cagagtaaaa cctaatggat gactgtggtg
    13801 cggttttgca taatattgaa accaaatggc tttatgattt tctgaccctg gaaaaatgcc
    13861 gcaatttttc ccaggcggca gtcagtcgca acgtctcgca accggcattc agccgccgca
    13921 tccgtgcact ggaacaggcg attggcgttg aattgtttaa ccgccaggtg acgccactgc
    13981 aactctcgga acaagggaaa atctttcatt cgcagatccg ccatctgttg caacagttag
    14041 aaagtaacct ggcagagctg cgtggcggca gcgattacgc gcaacgtaaa atcaagatag
    14101 ccgctgcaca ctctctttcc ctcggtctgt taccgtccat tatcagccag atgccgccgc
    14161 tctttacctg ggcaattgaa gccattgatg ttgatgaagc ggtcgataaa ctgcgtgaag
    14221 ggcaaagtga ctgtattttt tcctttcacg acgaagattt gctggaagcg ccgtttgacc
    14281 acattcgctt atttgaatct caattgttcc ccgtttgcgc cagtgacgaa cacggagaag
    14341 cactttttaa cctcgcgcag ccacactttc cgttactgaa ttacagccgc aactcctaca
    14401 tggggcgatt gattaatcgc accctgacgc gccacagtga gttaagtttc agcacctttt
    14461 ttgtctcttc gatgagcgag ctattaaagc aggttgccct cgacggctgt gggattgcct
    14521 ggctgccgga atacgctatc caacaagaaa ttcgcagcgg gcagctcgtt gtgctgaatc
    14581 gggacgaact ggtgatcccg atccaggcct acgcataccg gatgaatact cgcatgaatc
    14641 ccgttgccga acgcttctgg cgtgaactgc gcgagctgga gattgtgctt agctgacatt
    14701 ctccagcatc gcactacacc aaccgccatt atcccagacc agatcaaacg ttagcggttc
    14761 atcgtcgtgg tacagcatta tctcaaggct gatcgccagc gtgtggctgt acgcttcttt
    14821 cagattgacc tgcaacgctg caatccgtgg ctcatgaaat aacagtgcag aacgcagctg
    14881 acggcaatac cagctctgga gatgtttgtc gctctggtta tgcacttgcc actcgggcat
    14941 gcctgaaaca agtaatgacg gtaatgccga atgaggagaa ttaaaaagag tgaggagatc
    15001 gctgcatacc gatttacgca gcaatgacgt tttattgcct gaaatttcag gtaaaacagc
    15061 ctgaagtgat tggcgcatca ttttgcgcac tcctgacgtt tagcaacata tgcggcttgc
    15121 cctgaacacc gggcaccact gtattgatgc tcattttcca tagccattcc cttttgttca
    15181 ttacatccat gtcagtctga gacaggcctg aatcctgtca gcaaagtata aaaccttact
    15241 tgcctatgaa tatctactga cttttcagga aaaaagtcat gatttgataa aaatcatcaa
    15301 actcaaaaaa aaaccgctag tatattctga atattccgaa tagttacgta gatgaaaata
    15361 cttgtcaccg ccgctactta attccggtgg gaaattattt atttccgcat aatcagccat
    15421 gtattatttg gaacaatt
//
LOCUS       DAPGWD010000100        15344 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPGWD010000100 DAPGWD010000000
VERSION     DAPGWD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.101"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            338..1969
                     /gene="oppA"
                     /locus_tag="RHX88_003015"
     CDS             338..1969
                     /gene="oppA"
                     /locus_tag="RHX88_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDV2231680.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2055..2975
                     /gene="oppB"
                     /locus_tag="RHX88_003016"
     CDS             2055..2975
                     /gene="oppB"
                     /locus_tag="RHX88_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDV2231681.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2990..3898
                     /gene="oppC"
                     /locus_tag="RHX88_003017"
     CDS             2990..3898
                     /gene="oppC"
                     /locus_tag="RHX88_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDV2231682.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3910..4923
                     /gene="oppD"
                     /locus_tag="RHX88_003018"
     CDS             3910..4923
                     /gene="oppD"
                     /locus_tag="RHX88_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDV2231683.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4920..5924
                     /gene="oppF"
                     /locus_tag="RHX88_003019"
     CDS             4920..5924
                     /gene="oppF"
                     /locus_tag="RHX88_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDV2231684.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5977..6306)
                     /locus_tag="RHX88_003020"
     CDS             complement(5977..6306)
                     /locus_tag="RHX88_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDV2231685.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6341..7801)
                     /gene="cls"
                     /locus_tag="RHX88_003021"
     CDS             complement(6341..7801)
                     /gene="cls"
                     /locus_tag="RHX88_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDV2231686.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7944..8117
                     /locus_tag="RHX88_003022"
     CDS             7944..8117
                     /locus_tag="RHX88_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDV2231687.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8172..9425)
                     /gene="kch"
                     /locus_tag="RHX88_003023"
     CDS             complement(8172..9425)
                     /gene="kch"
                     /locus_tag="RHX88_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDV2231688.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(9725..10021)
                     /locus_tag="RHX88_003024"
     CDS             complement(9725..10021)
                     /locus_tag="RHX88_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDV2231689.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10245..10964
                     /gene="tonB"
                     /locus_tag="RHX88_003025"
     CDS             10245..10964
                     /gene="tonB"
                     /locus_tag="RHX88_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDV2231690.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11004..11402)
                     /gene="yciA"
                     /locus_tag="RHX88_003026"
     CDS             complement(11004..11402)
                     /gene="yciA"
                     /locus_tag="RHX88_003026"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDV2231691.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11507..12046)
                     /locus_tag="RHX88_003027"
     CDS             complement(11507..12046)
                     /locus_tag="RHX88_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDV2231692.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12076..12819)
                     /locus_tag="RHX88_003028"
     CDS             complement(12076..12819)
                     /locus_tag="RHX88_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDV2231693.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(12847..12936)
                     /locus_tag="RHX88_003029"
                     /pseudo
     CDS             complement(12847..12936)
                     /locus_tag="RHX88_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13176..13814
                     /gene="ompW"
                     /locus_tag="RHX88_003030"
     CDS             13176..13814
                     /gene="ompW"
                     /locus_tag="RHX88_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDV2231694.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(13874..14380)
                     /locus_tag="RHX88_003031"
     CDS             complement(13874..14380)
                     /locus_tag="RHX88_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV2231695.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(14426..14926)
                     /locus_tag="RHX88_003032"
     CDS             complement(14426..14926)
                     /locus_tag="RHX88_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV2231696.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLAINNVNK
                     KAENKA"
     gene            complement(15012..15191)
                     /locus_tag="RHX88_003033"
     CDS             complement(15012..15191)
                     /locus_tag="RHX88_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV2231697.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         4084 a         3594 c         3709 g         3957 t
ORIGIN      
        1 aaaagagaat tgcttaacaa ttttgcaaaa tgtattggcg agtaagaacc gcatttggta
       61 ctttccgggc aaccgccaga cgattattta ttggtaatga gaataattaa caattaaaga
      121 gcgtcgcgaa agaataatgt gtctcgacag gggagacaca gtacgaatcg acataaggtg
      181 atcgtctgaa tcaccagaat aaataaagtc ggtgatagta atacgtaacg ataaagtaac
      241 ctgacagcag aaagtctccg agcctgtgca gggtcccaat ccgggattac acatgctggt
      301 taataccagt aattataatg agggagtcca aaaaacaatg accaacatca ccaagagaag
      361 tttagtagca gctggcgttc tggctgcgct aatggcaggg aatgtcgcgc tggcagctga
      421 tgtacccgca ggcgtcacac tggcggaaaa acaaacactg gtacgtaaca atggttcaga
      481 agttcagtca ttagatccgc acaaaattga aggtgttccg gagtctaata tcagccgaga
      541 cctgtttgaa ggcttactgg tcagcgatct tgacggtcat ccagcacctg gcgtcgctga
      601 atcctgggat aataaagacg cgaaagtctg gaccttccat ttgcgtaaag atgcgaaatg
      661 gtctgatggc acgccagtca cagcacaaga ctttgtgtat agctggcaac gttctgttga
      721 tccgaacact gcttctccgt atgccagtta tctgcaatat gggcatatcg ccggtattga
      781 tgaaattctt gaagggaaaa aaccgattac cgatctcggc gtgaaagcta ttgatgatca
      841 cacattagaa gtcaccttaa gtgaacccgt tccgtacttc tataaattac ttgttcaccc
      901 atcaacttca ccggtgccaa aagccgctat cgagaaattc ggcgaaaaat ggacccagcc
      961 tggtaatatc gtcaccaacg gtgcctatac cttaaaagat tgggtcgtaa acgaacgaat
     1021 cgttcttgaa cgcagcccga cctactggaa caacgcgaaa accgttatta accaggtaac
     1081 ctatttgcct attgcttctg aagttaccga tgtcaaccgc taccgtagtg gtgaaatcga
     1141 catgacttat aacaacatgc cgatcgaatt gttccagaag ctgaaaaaag agatcccgga
     1201 cgaagttcac gttgatccat acctgtgcac ttactattac gaaattaaca accagaaacc
     1261 gccattcaac gatgtgcgtg tgcgtaccgc actgaaacta ggtatggacc gcgatatcat
     1321 tgttaataaa gtgaaagcgc agggcgacat gcccgcctat ggttacactc caccgtatac
     1381 tgatggcgca aaattgactc agccggaatg gtttggctgg agccaggaaa aacgtaacga
     1441 agaagcgaaa aaactgctgg ctgaagcggg ttataccgca gacaaaccgt tgaccatcaa
     1501 cctgttgtat aacacctccg atctgcataa aaagctggcg attgctgcct cttcattgtg
     1561 gaagaaaaac attggtgtaa acgtcaaact ggttaaccag gagtggaaaa cgttcctcga
     1621 cacccgtcac cagggtactt ttgatgtggc ccgtgcaggc tggtgtgctg actacaacga
     1681 accaacttcc ttcctgaaca ccatgctttc gaacagctcg atgaataccg cgcattataa
     1741 gagcccggcc tttgacagca ttatggcgga aacgctgaaa gtgactgacg aggcgcagcg
     1801 cacagctctg tacactaaag cagaacaaca gctggataag gattcggcca ttgttcctgt
     1861 ttattactac gtgaatgcgc gtctggtgaa accgtgggtt ggtggctata ccggcaaaga
     1921 tccgctggat aatacctata cccggaatat gtacattgtg aagcactaat ggcaatacgt
     1981 ggggcaggag tgtcctgctc cacggtgtct gatttttatc gcattacaga aggcacaggc
     2041 cagaaggtag ggcaatgtta aaatttattc tacgtcgctg tctggaagcg attccgacgc
     2101 tatttattct tattactatt tcgttcttta tgatgcgcct cgcgccggga agccctttta
     2161 ccggcgaacg tactttaccg cctgaagtga tggccaatat cgaagcgaaa tatcatctta
     2221 atgatccgat catgacacag tatttcagct acctgaaaca actggcgcac ggtgatttcg
     2281 gtccatcgtt taaatataaa gattattcgg tcaatgacct ggtggcatcc agttttcccg
     2341 tttctgccaa actgggagcc gcagcatttt tccttgcggt aatactgggt gttagtgctg
     2401 gcgttattgc cgcattaaaa caaaacacca aatgggacta taccgtgatg gggctggcaa
     2461 tgaccggggt tgttatcccc agttttgtgg ttgcgccatt attagtcatg atatttgcga
     2521 tcattttgca ttggctgccg ggcggtggct ggaatggtgg ggcgcttaaa ttcatgatat
     2581 tgccgatggt ggcgttgtca ctcgcctata tcgccagtat tgcgcgtatt acccgtggct
     2641 ctatgattga agtattacac tccaatttta ttcgtactgc ccgggcgaaa gggttaccta
     2701 tgcggcggat cattttacgc cacgcattaa aacctgctct gttacccgtg ctctcctata
     2761 tgggccccgc atttgtcggc attattaccg gttctatggt tatcgaaacc atttatggtt
     2821 tgccggggat tgggcaactg ttcgttaatg gcgcattgaa ccgtgactat tccttagtgt
     2881 taagcctgac catcctggtt ggtgctttaa ccattttgtt taatgccatt gtcgatgtgc
     2941 tatatgcggt tatcgacccg aaaatccgtt actgatactg gagctcgcga tgatgttaag
     3001 taagaaaaac agcgagacgc tggaaaattt cagtgaaaag ctggaggtcg aagggcgcag
     3061 cttgtggcag gacgcacgtc gacgttttat gcataaccgt gcggcggttg ccagtctgat
     3121 agtgctggtg ctgatcgcgt tatttgtaat cctggcaccg atgctttcgc agtttgccta
     3181 tgacgatact gactgggcga tgatgtccag cgccccggat atggagtccg gtcactactt
     3241 tggtactgac tcatccggtc gcgacctgct tgtgcgcgtt gcgattggcg ggcgtatctc
     3301 actcatggtc ggtgttgctg cggcactggt ggcagtggtc gtcgggacac tttatggttc
     3361 gctttccggt tatctgggcg gtaaagtgga ttcggtaatg atgcgtctgc tggaaatcct
     3421 caactccttc ccattcatgt tcttcgtcat tttgctggtg acctttttcg gtcaaaacat
     3481 cctgctgatt ttcgtggcga taggcatggt ttcctggctg gatatggctc gtattgtgcg
     3541 tgggcaaacc ctgagtctga agcgcaaaga gtttattgag gcggcacaag ttggcggtgt
     3601 atcgacatcg ggcattgtta ttcgccacat tgtgccgaat gtactcggtg tggtggtggt
     3661 ctacgcatca cttctggtgc ccagcatgat cctctttgaa tctttcctta gcttcctggg
     3721 gttgggtacg caagagccgt taagcagctg gggtgcattg ctgagtgatg gcgcgaactc
     3781 gatggaagtc tctccatggt tattgttgtt cccagcggga ttcctcgtgg tgacgctgtt
     3841 ttgtttcaac tttatcggcg atggcttgcg tgatgccctc gacccgaaag atcgttaagg
     3901 agtgcagcca tgagcgtaat tgaaactgca actgtgccgc tcgcacaaca acaggctgac
     3961 gcactgctga acgtgaaaga tttgcgtgtc acctttagta ccccggacgg cgacgtcacg
     4021 gcggtcaatg atttgaattt ttccctacgt gccggagaaa cgctgggtat tgtaggtgag
     4081 tctggttcgg gtaaatcgca aactgcattt gcgttgatgg gcctgttggc tgccaacgga
     4141 cgtattggcg gatcggcaac cttcaatggg cgtgaaatcc tcaatttgcc agagcatgaa
     4201 ctcaataaac tgcgcgctga acaaatctca atgatttttc aggacccaat gacttcgttg
     4261 aatccctata tgcgcgtcgg tgagcagtta atggaagtgc tgatgctgca taagaacatg
     4321 agcaaagctg aggcgtttga agagtcggtg cggatgctcg atgcggtaaa aatgccggaa
     4381 gcgcgtaaac gcatgaaaat gtacccgcac gaattttctg gcggcatgcg tcagcgagtc
     4441 atgattgcga tggcattgct atgtcgacct aagctactga ttgcggatga acccactact
     4501 gcgctggacg tcaccgtaca ggcgcagatc atgacgctat taaatgaact gaagcgggaa
     4561 tttaataccg ccatcattat gatcacccac gatcttggcg tggtggcggg gatctgtgac
     4621 aaagtgctgg taatgtacgc cgggcgcacg atggaatatg gcaatgcgcg cgatgtcttt
     4681 tatcaacccg ttcatcctta ttctatcggt ttgctcaacg cggtgccgcg tcttgatgcg
     4741 gaaggtgaaa caatgttgac catcccgggt aatccgccaa acttgctgcg attaccgaaa
     4801 ggttgcccgt tccagccacg ttgtccacat gcgatggaaa tttgtagtag cgcaccgccg
     4861 ctggaagagt ttacgcctgg ccgtctgcgt gcttgcttta aaccggtgga ggaactgtta
     4921 tgaatgctgt aactgaagga agaaaagtcc tccttgaaat cgccgatctt aaagtgcact
     4981 ttgaaatcaa agatggcaaa cagtggttct ggcaaccgcc gaaaacgctc aaagccgtcg
     5041 atggtgtcac tcttcgcctg tatgaagggg aaacattagg tgtggtaggg gaatcgggat
     5101 gcggtaagtc cacctttgct cgcgccatca tcggtttggt caaggcgacc gacggtcatg
     5161 ttgcctggtt aggtaaagag ttgctgggca tgaagcccga tgaatggcgt gccgttcgca
     5221 gtgatattca gatgattttc caggatccgt tggcatcgct aaacccgcgt atgaccatcg
     5281 gcgagatcat cgctgaacca ctgcgtactt atcatccgaa aatgtcacgc caggaagttc
     5341 gcgagcgcgt gaaggcgatg atgctgaaag tcgggttatt gcctaacctg attaaccgct
     5401 atccgcatga gttctccggt gggcagtgcc agcgtatcgg gattgctcgt gctcttattc
     5461 ttgaaccgaa gctgattatc tgcgatgagc cggtttcggc gctggacgtg tcaattcagg
     5521 cgcaggtggt caacctgctc cagcagctgc aacgtgagat gggattgtca ttaattttta
     5581 tcgctcatga cctggccgtg gtaaaacaca tttccgatcg tgtgttggtg atgtatctcg
     5641 gccatgcggt agaactgggg acctatgatg aggtctacca caatccacta catccttaca
     5701 ccagggcatt gatgtcggca gtccccatac ctgatccgga tctggagaag aacaaaacca
     5761 tccagttact ggaaggggaa ttaccgtcgc cgatcaaccc gccttccggt tgtgttttcc
     5821 gtacccgttg cccgattgcc ggtccggagt gcgccaaaac acgtcctgtt ctggagggga
     5881 gtttcagaca cgccgtttct tgcctgaaag tcgatccgct ttaaacacaa taagggctga
     5941 cagttgtcag ccctttttca cgccgaaagc gattatttat tcccgccaga tgatatggca
     6001 aagtttgtga tctttttcgc ggcataacag aatgcgggcg aaaacatcgt tgatttcacc
     6061 atcttcactg tccgccagac caatcaccac ttcggcaaaa aagtctgggt tcaaatcgaa
     6121 atcaacatgt tcctgccagt cttccgccgg atcgaataac tccgcgccgc cgcgctcttc
     6181 aaactgaaga ttgaacagca gaacatcagc tggatcgaga ttgtcggcag ccagttcgag
     6241 aaaaatatcg taggcctgct caagcgtttc atcttcagtc aggcgattgt ttagatccat
     6301 atccatagtc actacctgtt taacctctgt tggcgacgtt ttacagcaac ggactgaaga
     6361 agtaaaacag tcgctcggcg acacgttgcc ataatggacg ttttagccat aaacgggcat
     6421 cgagcagacg tgaacgcgaa atataatcgt cctgaacggc ggcaaggtct gcaccaaaac
     6481 ctttatcgtc gattgccagg gtaatctcga aattcagcca cagactacgc atatcaaggt
     6541 taacggtgcc aaccagactt agttcgccat cgaccagcac gctcttggta tgcagtaacc
     6601 cgccttcaaa ctgataaatt ttaaccccag cagcaagaag ttccgtaaag aatgcgcgac
     6661 tggcccagcc gaccagcatc gagtcatttt ttcgcggaag gataatactg acatccaccc
     6721 cgcgctgcgc cgccgtgcaa atcgcatgaa gtaaatcatc gcttggcaca aagtagggcg
     6781 tggtcatgat caaatattca cgcgccgaat aagccgcagt caataatgcc tggtgaatga
     6841 gatcttccgg aaagccgggg ccagaagcaa ttgtgtgaat ggtgtgaccg ctggcctgtt
     6901 caaacggcat aatattgaca tctggtggtg gcggcagaat acgttttccg gtttcaatct
     6961 cccagtcgca ggaataaata atccccatcg cggtggcgat ggggccttcc atacgcgcca
     7021 tcagatcaat ccattgccct acgcccgcat cttgtttgaa gtagcgagga tcgaccatat
     7081 tcatgctgcc ggtgtacgcg atgtaattat cgatcatgat catcttgcga tgttggcgca
     7141 ggtccatacg gcgtaaaaac acacgcatca gattgacctt taaggcttcg accacttcaa
     7201 taccggcatt acgcattagc tcgggccacg ggctgcggaa aaaagccaca ctcccggcgg
     7261 agtcgagcat caatcggcaa tgaatgccgc gtcgcgcagc ggccattaat gattcagcca
     7321 cctggtccgc catgccgccg ggctgccaga tataaaacac catctcaata ttatggcgcg
     7381 cgagctggat gtcgcggatt aacgcctgca tcacatcatc tgactcggtc atcagttgta
     7441 gctgattccc tttgacccca gcgatcccct gacgacgctc gcaaagcttg aataatggcg
     7501 cagcgacact gctattttct tcggcgaaga tatgcttaca ggctttaagg tcgttaagcc
     7561 attttgcggt ggaaggccac atcgctctgg cgcgctcagc gcggcgtttg cctaaatgga
     7621 gctcgccaac ggcaagatag gcaataattc cgactaacgg cagaatgtaa ataatcaaca
     7681 gccaggccat cgcggaggga actgcgcgtc gtttcattag aatgcgtaaa gttacgcctg
     7741 caatgagcaa ccagtatccc agaatggcca accaactcac caacgtataa acggttgtca
     7801 tagattagaa atccttttga aagcgcattg ttatgagttt acgcatatgg attcatctgg
     7861 caaataaaaa cgcgggaaaa gcactggtca gcgtcggggg tgggggtata atgaccattc
     7921 tgttattgca tagagtagtt aacatgaagc ggagtagaac ggaagtgggg cgctggcgca
     7981 tgcagcgtca ggctagccga cgtaaatcgc gttggcttga ggggcaatcg cgccgaaata
     8041 tgcgtatcca cagcatcagg aagtgcattc taaacaaaca gcgtaactcg ttattgtttg
     8101 cgatctacaa tatctaaata tactgggcac cattgcggtg cccaataagt ttactctttc
     8161 gtctttaaac tctatttttg cactgattct ttactgtcag ctttcgtttc ttgttcatcg
     8221 ttatcgctga aaatgccatg accggagttc aacaacattg aaacgagcat atcattatta
     8281 atctcttcac catttaaaac tcgcgccaga atttcgctgc caaacagttg cggtgagaga
     8341 atgatatccg gatgtaccat cttaatctta ttcaggtttt tgctatcact gacggcgaga
     8401 actgttttga catcactgct catatctttt gccgagagta caacaaacgc gttatctgca
     8461 tcgttatcac tcagcgccag aatggcccgg caacgatcga ttcccgcttt ctttaatact
     8521 gaactgtcat tactgtcacc ggggataaca tcagcgttat cgcctaagcg ttgctcaagt
     8581 tgcttgatat catcttcagg caagttgctg ataaccgtta cgttttgtcc gcgttgattc
     8641 agttgcagga tcgtattgat ggcgagaatc gaatgtccgc aaacaataaa atgatcttta
     8701 cgatgcattg tatgattgtt tccttttaca agtttgttga atcccccgcg gataagcgga
     8761 ccaaaaattg aggtcataga tgtggcaaaa acggtaatgc cggaaataat gaccgaaata
     8821 gtgaacaatc gtgctgattc agaaacaggg acaatatcgc catagccgac ggttgacatg
     8881 gtttctatcg aaaaatagaa cgcggtcatc aaactttcta ttcgcggatt aaaaccttcg
     8941 cttaaataaa gcgcaccgta ggttgagtaa aacagtaatg tcgtgaaact aataaatgca
     9001 aaaattgtcc cggctgcggc gctactgtgg gagaagtctt tgcgcagtat cagcaaaaac
     9061 accagcgtaa aaatgcaaaa tccaatacta aatttcagcc agggataaaa atgcagggtg
     9121 taaattagcg ctatcaacaa caaaatgata ctgattgccc atgcgagctt tgcccggaac
     9181 agtaagccaa tggagttcag gacaagaaag acgccgagca taaatagtgg ggcgtttgcc
     9241 agcaaggaga gatccaactc tgaaaatgct ttgatatgga aaatatcaag caaattgacc
     9301 gacataccat agattgtttt aaaaataagc aatccattta aaaagacggc cagcgcgaga
     9361 atgtcgtgcc gaagggtcac ccataaattt gttgctgttt gtttgaatgt agcccagtga
     9421 ctcactgata cctctgtaac cacaattttg tttaattatc ttagcgtgca cattttctga
     9481 acatacatgc agcgcgtgaa tgtgttaaac aatagtagat gactttatcc gcagtaatca
     9541 aaggatttga gcgagcaact gtacctcagg aaaggtcatt tttcctgaat atgctcacat
     9601 catataaaga aatacagata aagttattat ctgcttgtgg tagtgaacgc actgaccggc
     9661 tataaggaaa ggccaaacaa gaacacggtt gcaaaaaccg tgcccttaaa tattgaatct
     9721 ctattcagaa cactttctta aatggtttca ctgaaacgtg ttcatagaca cctgccgcta
     9781 cgtacgggtc agcatcggcc caggcctgag ctgcttccag cgattcaaat tcagcaataa
     9841 cggttgagcc agtaaatccc gcagcccctg gatcgttact gtctaccgct ggcattggac
     9901 cagctgtcaa caaacgacct tcatcatgca gtaactgtaa acgtgctaaa tgtgccggac
     9961 gaacggaaag gcgtttttcg aggctatcag ctttgtcttg agcgtaaata acatacaaca
    10021 cgggcacaac tccttgttcg ggaaagttgt aaagtacgtt atttgaaagg gcgaagatct
    10081 gcaacggaaa gatgatgtct ttgttaagac catgcataaa gtaaggtcaa ttacgcccaa
    10141 aatgacattt tcactgatcc tgatcgtctt gccttattga atatgattgc tatttgcatt
    10201 taaaatcgag acctggtttt tctactgaaa tgattatgac ttcaatgacc cttgatttac
    10261 ctcgccgctt cccctggccg acgttacttt cggtctgcat tcatggtgct gttgtggcgg
    10321 gtctgctcta tacctcggta catcaggtta ttgaactacc tgcgcctgcg cagccaattt
    10381 ctgtcacgat ggttgcgcct gctgatctcg aaccgccaca agccgttcag ccgccaccgg
    10441 agccggtggt agagccagaa ccggaacctg agccgatccc cgaaccgcca aaagaagcac
    10501 cggtggtcat tgaaaagccg aagccgaaac ctaagccaaa accgaagccg gtgaaaaagg
    10561 tacaggagca gccaaaacgc gatatcaaac ctgtagagtc gcgtccggca tcaccgtttg
    10621 aaaatacggc acccgcgcgc cccacatcaa gtacggcaac ggctgcaacc agcaagccgg
    10681 ttaccagtgt ggcttcagga ccacgcgcat taagccgtaa tcagccgcag tatccggcac
    10741 gagcacaggc attgcgcatt gaagggcagg ttaaagttaa atttgacgtc acgccggatg
    10801 gtcgcgtgga taacgtacaa atcctctcag ccaagcctgc gaacatgttt gagcgtgagg
    10861 tgaaaaatgc gatgcgcaga tggcgttatg agccgggtaa gccaggcagt gggattgtgg
    10921 tgaatatcct gtttaaaatt aacggcacca ccgaaattca gtaagcagaa agtcaaaagc
    10981 ctccgaccgg aggcttttga ctattactca acaggtaagg cgcgaggttt tccttcagga
    11041 tcaaccgcga catacttaaa taatgcttct gtcgctttat agcgttgccc gattggttca
    11101 gacgctactt ttttcaccca cacttcaata ttaatgctga ccgatgtcgt ccctttctgg
    11161 acacagcgtg catagcagca caccacatcg ccgaccgcaa ccggccgtaa gaaagtcatt
    11221 ccttcaaccc gcacagtcac tacgcgaccg tgggcaattt ctttcgccag aatagcgccg
    11281 ccaatatcca tttgtgacat taaccaacca ccaaagatgt caccattggc attggtatcg
    11341 gcgggcatgg ctaaagtacg taaaacaaga tcgccctgag ggacgttatg tgttgtagac
    11401 atggtaaaac cgacttaaaa ggaaaatcac aggcgcgatg ctactatgat tttcctgagg
    11461 agaacagagg agatacagcg ccagccccga aggactggca gtctggttag gatttatctt
    11521 cctgcggcat gtggcggtag atatagatac cgcttaacaa tgtgaagatt aaggtaaggg
    11581 cggtcaggcc aaagacttta aagttgaccc aaatattttg cggcagccag aatgcgatgt
    11641 agatgtttgc cagaccgcaa aggataaaga aaacagccca agccagattt agcttcgacc
    11701 ataccggttg cggcagcgtg agttctttac ccagcatccg ctgaattagc ggctttttca
    11761 tcacccattg gctgactaac agggctcccg caaacagagc ataaatgact gtaaccttcc
    11821 atttaataaa ctcatcattg tggaagaaca gcgtcaagcc accgaagacg accaccagaa
    11881 caaaagtgat cagggccatc ttctcaacct tacgaaagcg aacccagcta tagataagca
    11941 caatcgccgt ggcgacgatc agcgccgcag tagccgcata gatgtcataa atcttgtaaa
    12001 acgcgaaaaa gacaaccagc ggtaaaaaat caagaaactg cttcatttta cgattccgta
    12061 atcaaatgct taaggttatt ggcgaatcaa catatacagg cggaacagat agatgagcaa
    12121 tatggcagaa atcaggttgc tcaaggtgtt cgccagtacg gcaccaattt ccggggttaa
    12181 tgcggcaaaa gaagaggcaa aaagcagcaa cagtgttttt gccagcaacc agctcagtac
    12241 tgcgggtgcc accagacgca tattcgccca agtcagccgc atactgctac gcatcgaggc
    12301 aaaaatgccc attttgtcct gtaccagcat caccggtgcc agggccagta aaatggccat
    12361 gataattccc ggaacgacca ccagcatgat gccaatctgt actaaaaggg tagtcagaaa
    12421 aatcagaata aataacttgg gcaatatcgg cgcactggca ccaatagctc gcagcgcact
    12481 gactctctga cccgcagaca ccagctggat aattaatatt acgcctccgg cgagaatggc
    12541 gttaccgatt aatcctgaaa aagtggacgc cgctgaagcc tgcagcaaaa tttgttgctg
    12601 ttccggtgac atattctgaa ccaggtcgaa caacccacta ctgccgctaa cgggcacgcc
    12661 gtcattgagc tgcgcaagct gtgcatcact gggtgagaaa acatgcccta acaccactgt
    12721 gataaacgca catagcaacg ataccaacag aatggtcata aattgattac ggaagaaatt
    12781 tccggtgtca cggtatacgg actgcgccgt gatagacatg cactctcctt gagttttatg
    12841 caggtgttaa ttagcgggca attgtaccct ggttaagcct cgggtggcag catcaggtct
    12901 tgtatggaaa agcatatctt tgtaaagcgg aagtaattgc tggccttcag gcgtgggcaa
    12961 accgcggcaa gtggcgaacg agttaatatt tgcgtagcga aaatatttaa aaattgattt
    13021 aaatcacgtt aaccaggatt ttcaatgcaa cttctaaatt aatccagatc aataaagggt
    13081 gaattatcat atgtaatgtg atctatgtag gatcatttgt tactccaatg taggtatatt
    13141 cgtcacgttt ttataaccat aacgacggag cggatatgaa aaagttaaca gtggcggctt
    13201 tggcagtaac aactcttctt tctggcagtg cctttgcgca tgaagcaggc gaatttttta
    13261 tgcgtgcagg ttctgcaacc gtacgtccaa cagaaggtgc tggtggtacg ttaggaagtc
    13321 tgggtggatt cagcgtgacc aataacactc aactgggctt gacgtttact tatatggcga
    13381 ccgacaacat tggtgtggaa ttactggcag cgacgccgtt ccgccataaa atcggcaccc
    13441 gggcgaccgg cgatattgca accgttcacc atctgccgcc aacactgatg gcgcagtggt
    13501 attttggtga tgccagcagc aaattccgtc cttacgttgg ggccggtatt aactacacca
    13561 ccttctttga taatggattt aacgatcatg gcaaagaggc ggggctttcc gatctcagtc
    13621 tgaaagattc ctggggagct gccgggcagg tgggggttga ttatctgatt aaccgtgact
    13681 ggttggttaa catgtcagtg tggtacatgg atatcgatac caccgccaat tataagctag
    13741 gcggtgcaca gcaacacgat agcgtacgcc tcgatccgtg ggtgtttatg ttctcagcag
    13801 gatatcgttt ttaattccgc acaaaaacga ccccgtaata tacggggtca ataaggacat
    13861 ggtataaagg gtattatttc ttcgcttcta cgccatcagt ttcagaacga attaaaaatt
    13921 tctcagttgt ttgcggaata tgctgaatca gccagtcggc catttgcttt tcctcattta
    13981 aaatcgcttc gatgatcgga attgagactg tatcaccggc attttttgct gctgctaata
    14041 gtgaggtgta acaggcgatt tcaaattgct cgaagacata tccgctaata gagcctttaa
    14101 ctatttcatc agaagggaat ataccaccga ttgactgccc aagcgcagcc attttactca
    14161 tggaatcttt aatgactgaa cgtgaaatgt cattacgatc aagaatagtt tccagttgaa
    14221 caatctggtt tttggtttca ctaagatgtt gttcaatacg agcgcgtagt tcaggataat
    14281 tatctatacg gctggccatg gattcaagca tagattcggc ttgcttttcc attgcgtggg
    14341 cgtcacgtaa ccagtcatga taatgttcaa tacgattcat gaaaatgtcc tctttataaa
    14401 atgactaaaa gttaaaattc atatttcagg ctttattttc ggctttctta tttacgttat
    14461 taatggccag atcagtcagt ttgatgtcgg tggccttttc ttcttccagg gtttctttca
    14521 gaagtttcgc tgctttacgg taacctaatt gttcagccag cgtcgctaat gtcccgtaac
    14581 tggcaatctc ataatgctcg actttctgtg ctgcggcaat cagtgcggca tcacgcactt
    14641 cgtttttctc ggtactttcg atgacctcat tagcttcttc aataagacct tccattgcca
    14701 cacatttcat gcgcttaatt ttcaggttcg attcggactc cacaacttga tcaatacgtt
    14761 caatctgtcc atgagtttcc tcgaggtgcg catgaaaagc ctgacttaat ttttcatttg
    14821 atgttgctct tgcgagtttt gccagtgccc gggttaattg tttttctgcg ctgtaggtat
    14881 ctgaaagcag gtgaataaat acatcttcaa tggtcttcat attcatattt ttctccagtg
    14941 aaatcactgc gagccatacg gcccgcagat gcaattatct ttatcagctg atattaataa
    15001 ttaacggtga atcaggattt acctgattta ttaccaccgc tttgttgacc gcctttttta
    15061 cccgcttcag atgcgcgttg cggatcattt ttaaaattac cgccgctatg ctgaccgcct
    15121 ttacggcctg cgtcggatgc cttctcacgg tcttcggcga aatttcctga accaccacga
    15181 tgttcggcca tgttatttct cccgttgcgt tgcattgttt cattaatatg agtgttgtgt
    15241 gtcgacactc attaaaatta gtcgctaatg agaattagtc aaattaagcg caacgagaag
    15301 atagagggaa aatatatttt gaggaacatt ctggatatat taac
//
LOCUS       DAPGWD010000101        15108 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPGWD010000101 DAPGWD010000000
VERSION     DAPGWD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.102"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(297..353)
                     /locus_tag="RHX88_003034"
     CDS             complement(297..353)
                     /locus_tag="RHX88_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV2231698.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            632..1879
                     /gene="mdtA"
                     /locus_tag="RHX88_003035"
     CDS             632..1879
                     /gene="mdtA"
                     /locus_tag="RHX88_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDV2231699.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLIGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLSSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            1879..5001
                     /gene="mdtB"
                     /locus_tag="RHX88_003036"
     CDS             1879..5001
                     /gene="mdtB"
                     /locus_tag="RHX88_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDV2231700.1"
                     /translation="MQMLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEELQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVTAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            5002..8079
                     /gene="mdtC"
                     /locus_tag="RHX88_003037"
     CDS             5002..8079
                     /gene="mdtC"
                     /locus_tag="RHX88_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDV2231701.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLNWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            8080..9495
                     /locus_tag="RHX88_003038"
     CDS             8080..9495
                     /locus_tag="RHX88_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2231702.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLVLDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNHRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            9492..10895
                     /gene="baeS"
                     /locus_tag="RHX88_003039"
     CDS             9492..10895
                     /gene="baeS"
                     /locus_tag="RHX88_003039"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDV2231703.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            10892..11614
                     /gene="baeR"
                     /locus_tag="RHX88_003040"
     CDS             10892..11614
                     /gene="baeR"
                     /locus_tag="RHX88_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDV2231704.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            11805..12137
                     /locus_tag="RHX88_003041"
     CDS             11805..12137
                     /locus_tag="RHX88_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDV2231705.1"
                     /translation="MAGWFELSESSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            12346..12642
                     /locus_tag="RHX88_003042"
     CDS             12346..12642
                     /locus_tag="RHX88_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDV2231706.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            12644..12940
                     /locus_tag="RHX88_003043"
     CDS             12644..12940
                     /locus_tag="RHX88_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2231707.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            13043..14404
                     /gene="yegQ"
                     /locus_tag="RHX88_003044"
     CDS             13043..14404
                     /gene="yegQ"
                     /locus_tag="RHX88_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDV2231708.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(14734..15051)
                     /locus_tag="RHX88_003045"
     CDS             complement(14734..15051)
                     /locus_tag="RHX88_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231709.1"
                     /translation="MKKIVAISLISIFIVSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         3400 a         4045 c         4063 g         3600 t
ORIGIN      
        1 ccacgatgat gagtagcttc atcatgaccc tttccttatt taaggcccct tcctcgggag
       61 gggctttccc gtttcagcgt cccgctgaaa tcgtcggctt acctcctttc gccatgcact
      121 tagtctatcg ctgacttgca gataaaatag tttccagtgt gattattgcg taacgtctcg
      181 caaagaattt ttgctggcag gatcgcagac ggcaaagcct gcggattgac aatcttatcg
      241 tgaaggcata ctttcaggag tgagggtaga gcggggtttc ccccgccctg gtagtcttag
      301 tatgctgggt agctaatcac taagagcacc acgatgatga gtagcttcat catgaccctt
      361 tccttattta aggccccttc ctcgggaggg gctttcccgt ttcagcatcc ggctgaaatc
      421 tgcggcttac ctcctttcgc cctgtatgta gtctatcgct aacgcgtaga taaaatagtt
      481 tcctgtgtta ttactggatg cgtgctcgca aatgtgcccg ccattcagac gattcccgat
      541 agtgtttcat aattcctcca tttttctccc ttattggctg gctacactag tatcattccg
      601 cgaaacgttt caggaagaga aactcttaac gatgaaaggc agttataaat cccgttgggt
      661 aatcgtaatc gtggtggtta tcgccgccat cgccgcattc tggttctggc aaggccgcaa
      721 tgactcccag agtgcagccc caggggcgac gaaacaagcg cagcaatcgc cagcgggtgg
      781 tcgccgtggt atgcgttccg gcccattagc cccggttcag gcggcgaccg ccgtagaaca
      841 ggcagttccg cgttacctca tcgggcttgg caccattacc gccgctaata ctgttacggt
      901 gcgcagccgc gtggacggtc aactgatggc gttacatttc caggaaggcc agcaggtcaa
      961 agcaggcgat ttactggcag aaattgaccc cagccagttc aaagttgcat tagcacaagc
     1021 ccagggccaa ctggcaaaag ataaagccac gcttgctaac gcccgccgtg atctggcgcg
     1081 ttatcagcaa ctggcaaaaa ccaatctcgt ctcccgtcag gagctggatg cccaacaggc
     1141 gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa gcaagcgtcg ccagcgcgca
     1201 gctgcaactc gactggagcc gcatcaccgc accagtcgat ggtcgcgttg gtctcaagca
     1261 ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc ggaattgtgg tgatcaccca
     1321 gacgcatcct atcgatttag tctttaccct gccggaaagc gatatcgcta ctgtagtgca
     1381 ggcgcaaaaa gccggaaaac cgctggtggt agaagcctgg gatcgcacca attcgaagaa
     1441 attaagtgaa ggcacgctgt taagcctcga taaccaaatc gatgccacaa ccggtacgat
     1501 taaagtgaaa gcacgcttta ataatcagga tgatgcgctg tttcccaatc agtttgttaa
     1561 cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg atcccaacgg ccgccctgca
     1621 aatgggcaat gaaggccatt ttgtctgggt gctgagtagc gaaaacaagg tcagcaaaca
     1681 tctggtgacg ccgggcattc aggacagtca gaaagtggtg atccgtgcag gtatttctgc
     1741 gggcgatcgc gtggtgacag acggcattga tcgcctgacc gaaggggcga aagtggaagt
     1801 ggtggaagcc cagagcgcca ctactccgga agagaaagcc accagccgcg aatacgcgaa
     1861 aaaaggagca cgctcctgat gcagatgtta cccccgagca gcacaggcgg cccgtcgcgc
     1921 ctgtttatta tgcgtcctgt ggccaccacg ctgctgatgg tggcgatctt actcgccggg
     1981 attatcggtt atcgcgccct gcccgtttcg gcgctgccgg aagtggacta tccgaccatt
     2041 caggtggtca cgctctaccc aggtgccagc ccggatgtca tgacctctgc cgttaccgcg
     2101 ccgctcgaac gccagttcgg gcagatgtct ggcctgaaac agatgtcgtc gcaaagttcc
     2161 ggcggtgcgt cagttatcac tttgcagttc cagctaacat taccgctcga tgtcgccgag
     2221 caggaagtgc aggccgcgat taacgctgcg accaacttgt tgccgagcga tctgcctaac
     2281 ccgccggttt acagcaaagt gaacccggca gatccgccga tcatgacgct cgccgtcacc
     2341 tcaaccgcca tgccgatgac ccaggtagaa gatatggtgg aaacccgcgt cgcgcagaaa
     2401 atctcgcaga tttccggtgt cggactggtg acgctttccg gcggtcagcg tccggctgtt
     2461 cgcgtcaaac ttaacgccca ggcgattgcc gccctcggcc tgaccagcga aaccgtgcgc
     2521 accgccatta ccggagctaa cgtcaactcg gcaaaaggta gcctcgacgg cccttcccgt
     2581 gcggtcacgc tttccgctaa cgaccagatg caatccgccg aagagtatcg ccagctaatc
     2641 atcgcctacc agaacggcgc accgattcgt ctgggcgatg tcgcaaccgt agagcaagga
     2701 gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac aggccattgt gatgaatgtt
     2761 cagcgccagc ccggtgctaa cattatctcc accgccgaca gcatccggca gatgctgcca
     2821 cagctcaccg agagtctgcc gaaatcggtg aaggtgacag tgctttccga tcgcaccacc
     2881 aatattcgcg catccgtcga tgatactcag tttgaattga tgatggctat cgcgctggta
     2941 gtcatgatca tctacctgtt tttgcgcaat attccggcga ccatcattcc cggtgttgct
     3001 gtaccgctgt cgttaatcgg cacttttgcg gttatggtgt ttctcgattt ttcaatcaat
     3061 aacctgacac tgatggcgtt aactatcgcc accggattcg tggtcgatga cgccatcgta
     3121 gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa aaccgttggc ggcggcgctc
     3181 aaaggcgcgg gtgaaatcgg ctttaccatt atctcgctga ccttctcgct gattgcggtg
     3241 ttgattccac tgctgtttat gggcgatatc gtcgggcgac tgttccgcga atttgctatt
     3301 accctggcgg tagcgatttt gatctcagcg gtggtgtcgc tgaccctgac accgatgatg
     3361 tgcgcgcgga tgctcagcca ggagtcgctg cgtaaacaga accgattctc ccgtgcctcg
     3421 gaaaaaatgt tcgacaggat aatcgccgcc tatggtcgtg gactggcgaa ggtgctgaat
     3481 catccgtggc tgaccttaag cgtggcgctc agcacgctgc tgcttagcgt tctcttatgg
     3541 gtgttcattc cgaaaggttt cttcccggta caggacaacg gcattattca gggcactttg
     3601 caggcaccgc aatccagctc ctttgccaat atggcccagc gacaacgcca ggtcgcggac
     3661 gtgattttgc aggatccggc agtgcaaagc ctgacctcat ttgttggcgt tgatggcact
     3721 aacccgtcgc tgaatagtgc acgtttacaa atcaacctca aaccgttgga tgaacgtgat
     3781 gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg tagataaagt gccgggcgtc
     3841 gatctcttcc tgcaaccaac gcaggatctg actattgata ctcaggtcag ccgcacccag
     3901 taccagttta ccttgcaggc cacctcactg gatgcgctca gtacctgggt gccacagttg
     3961 atggaagaac tccagcaact gccacagctt tctgatgtct ccagcgactg gcaggacaaa
     4021 gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg gatcagcatg
     4081 gcggatgtcg ataacgccct gtacaacgcg tttggtcagc gactgatttc cactatttat
     4141 actcaggcta accagtatcg cgtagtgctg gagcacaaca ccgaaattac cccgggcctc
     4201 gcggcgctgg ataccattcg cctgaccagt agcgacggcg gcgtggtgcc gttaagctca
     4261 attgccaaag ttgaacagcg ttttgcgccg ctttccatca accatctgga tcagttcccg
     4321 gtaacgacca tctcctttaa cgtgccggat aactattcgc tgggcgatgc ggtgcaggcg
     4381 attatggaca ccgaaaagac gctgaatctg ccggtggata tcaccacgca attccagggc
     4441 agcaccctcg ccttccagtc ggcgctgggc agcactgtct ggctgattgt cacggcggtg
     4501 gtggcgatgt atatcgtgct cggcattctg tacgagagct ttattcaccc gatcaccatt
     4561 ctctcgacgc tacccaccgc aggagtcggc gcactgctgg cgttaatgat tgccggtagc
     4621 gaactggatg tgattgcgat tatcggcatt attttgctga tcggtatcgt gaagaagaac
     4681 gccatcatga tgatcgactt cgcgctggct gctgagcgcg agcaaggcat gtcgccgcgc
     4741 gatgccatct accaggcttg tctgttgcgt tttcgtccga tcctgatgac cactctggcg
     4801 gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga actgcgtcgt
     4861 ccgttaggta tcggcatggt cggcggcctg attgtcagcc aggtgctgac gctgtttacc
     4921 acgccggtga tttatctgct gttcgaccgc ctggcattgt ggaccaaaag tcgctttgct
     4981 cgccatgaag aggaggcgta agtgaagttt tttgccctct tcatttaccg tccggtggcg
     5041 acgattttac tgtcggttgc cattaccctg tgcggcatac tgggcttccg tatgctgccg
     5101 gtcgccccgc tgccgcaggt cgattttccg gtgattatgg tcagcgcctc gctgcccggt
     5161 gcgtcaccag aaacaatggc gtcttccgtt gccacgccgc tggagcgctc acttgggcgc
     5221 attgccggag tcagtgaaat gacctccagc agttcgctcg gcagcacgcg tattattttg
     5281 cagtttgatt ttgaccggga tatcaacggc gcggcgcgcg atgtgcaggc ggcgatcaac
     5341 gctgcacaaa gtttgctgcc cagcgggatg cccagccgtc cgacctatcg caaagctaac
     5401 ccgtcggatg cgccaattat gatcctcacg ctgacatccg atacttattc gcagggtgaa
     5461 ctgtacgatt ttgcctcgac gcagctggct ccgacgattt cgcaaatcga cggtgtcggt
     5521 gatgtcgatg ttggcggcag ctcgctgccc gccgtacgcg tcgggctgaa tccgcaggcg
     5581 ctgtttaatc agggcgtctc gctggacgac gtacgcaccg ccatcagcaa tgccaacgtg
     5641 cgtaaaccgc agggggcgct ggaagatggc actcaccgct ggcagatcca gaccaatgat
     5701 gagctaaaaa ccgccgccga atatcagccg ttgattattc attacaacaa cggcggcgcg
     5761 gttcgtctgg gcgatgtggc gacggtgacc gactcggtgc aggatgtgcg caacgccggg
     5821 atgaccaacg ccaaaccggc tattttgctg atgatccgca agctgccgga agccaatatt
     5881 atccagacgg tagacagcat ccgggcaaaa ttaccggagt tgcaggaaac cattccggcg
     5941 gcgattgatc tgcaaattgc tcaggatcgc tcccccacca ttcgcgcctc gctggaagaa
     6001 gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc tggtggtgtt tttattcctg
     6061 cgctcgggtc gcgccactat tattcccgcc gttgcggtgc cggtttcgct gattggtacg
     6121 tttgcggcga tgtacctgtg cggattcagt ctcaataacc tttcgttaat ggcgctcacc
     6181 atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc tggaaaacat tgcccgtcat
     6241 ctggaagcgg ggatgaaacc gctacaggcc gcgctacaag gtactcgcga agtcggcttt
     6301 acggtgctgt cgatgagtct gtcactggtg gcggtgttcc tgccgctgct gttgatgggc
     6361 ggattgccgg gccgactgtt acgcgaattt gccgtgacgc tttctgtcgc cattggtatt
     6421 tcgttgctgg tttctctgac attaacgcca atgatgtgtg gctggatgct gaaagccagc
     6481 aagccgcgcg agcaaaagcg actgcgtggt tttggtcgca tgttggtagc cctgcaacaa
     6541 ggctacggca agtcactgaa ctgggtgctc aatcataccc gtctggtggg cgtggtgctg
     6601 cttggcacca ttgcgctgaa tatctggctg tatatctcga tcccgaaaac cttcttcccg
     6661 gagcaggaca ctggcgtgtt gatgggcggg attcaggcgg atcagagtat ttcgtttcag
     6721 gcgatgcgcg gcaaattgca ggatttcatg aaaattatcc gtgacgatcc ggcagtggat
     6781 aatgtcaccg gctttaccgg cggttcacga gttaacagtg ggatgatgtt tatcaccctc
     6841 aagccacgcg acgaacgcag cgaaacggcg cagcaaatta tcgaccgtct gcgcgtaaaa
     6901 ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg tacaggatat tcgcgttggt
     6961 gggcgtcagt cgaacgccag ctaccagtac acgttgttat ccgacgacct ggcggcactg
     7021 cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt tgccggaact ggcggacgtg
     7081 aactccgatc agcaggataa cggcgcggag atgaatctgg tttacgaccg tgacaccatg
     7141 gcacggctgg gaatcgacgt acaagccgcc aacagtctgt taaataacgc cttcggtcag
     7201 cggcaaatct cgaccattta ccagccgatg aaccagtata aagtggtgat ggaagtggat
     7261 ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt tcgttatcaa taacgaaggc
     7321 aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg cgaatgcccc actatcggtg
     7381 aatcatcagg gattatcggc ggcctcgacc atttcgttta acctgccgac cggaaaatcg
     7441 ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt gccttcgacg
     7501 gtgcgcggca gttttgccgg cacggcgcag gtgttccagg agacgatgaa ctcgcaggtg
     7561 atcctgatta tcgccgccat cgccacggtg tatatcgtgc tgggtatcct ttacgagagt
     7621 tacgtacatc cgctgacgat tctctccacc ctgccctcgg cgggcgttgg agcgctgttg
     7681 gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc tgatagggat catgctatta
     7741 atcggcatcg tgaagaaaaa cgccattatg atggtcgatt ttgcgcttga agcccaacgg
     7801 cacggtaacc tgacgccgca ggaagctatt ttccaggcct gtctgctgcg ttttcgcccg
     7861 attatgatga ccaccctggc ggcgctgttt ggtgcgctgc cgctggtatt gtcgggcggc
     7921 gacggctcgg agctgcggca acccctgggg atcaccattg tcggcggact ggtaatgagc
     7981 cagctcctta cgctgtatac cacgccggtg gtgtatctct ttttcgaccg tctgcggctg
     8041 cgtttttcgc gtaaacctaa acaaacggta accgagtaaa tgacagatct tcccgacagc
     8101 acccgttggc aattgtggat tgtggctttc ggcttcttta tgcagtcgct ggacaccacc
     8161 atcgtaaaca ccgcccttcc ctcaatggcg caaagcctcg gggaaagtcc gttgcatatg
     8221 cacatggtca ttgtctctta tgtgctgacc gtggcggtga tgctgcccgc cagcggctgg
     8281 ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg ccatcgtgct gtttactctc
     8341 ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc acgcgcgtta
     8401 cagggcgttg gcggcgcgat gatggtgccg gtcggcagat tgacggtgat gaaaatcgta
     8461 ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt tacccggtca ggtcggtccg
     8521 ctgctcggtc cggcgctcgg cggtctgctg gtggagtacg cgtcgtggca ctggatcttt
     8581 ttgatcaaca ttccggtggg gattatcggt gcgatcgcca cattgctgtt aatgccgaac
     8641 tacaccatgc agacgcggcg ctttgatctc tccggatttt tattgctggc ggttggcatg
     8701 gcggtattaa ccctggtgct ggacggcagt aaaggtacag gtttatcgcc gctgacgatt
     8761 gcaggcctgg tcgcagttgg cgtggtggca ctggtgcttt atctgctgca cgccagaaat
     8821 aaccaccgcg ctcttttcag tctgaaactg ttccgcactc gtaccttttc gctgggcctg
     8881 gcggggagct ttgccggacg tattggcagt ggcatgttgc cctttatgac accggttttc
     8941 ctgcaaattg gcctcggttt ctcaccgttt catgccggac tgatgatgat cccgatggtg
     9001 cttggcagca tgggaatgaa gcgaattgtg gtacaggtgg tgaatcgctt tggttatcgt
     9061 cgggtactgg tagcgaccac gctgggtctg tcgctggtca ccctgttgtt tatgactacc
     9121 gccctgctgg gctggtacta cgttttgccg ttcgtcctgt ttttacaagg gatggtcaac
     9181 tcgacgcgtt tctcctccat gaacaccctg acgctgaaag atctcccgga caatctggcg
     9241 agcagcggca acagcctgct gtcgatgatt atgcaattgt cgatgagtat cggcgtcact
     9301 atcgccgggc tgttgctggg actttttggt tcacagcatg tcagcgtcga cagcggcacc
     9361 acacaaaccg tctttatgta cacctggctt agcatggcgt tgatcatcgc ccttccggcg
     9421 ttcatctttg ccagagtgcc gaacgatacg catcaaaatg tagctatttc gcggcgaaaa
     9481 aggagcgcgc aatgaagttc tggcgacccg gtattaccgg caaactgttt ctggcgattt
     9541 tcgccacctg cattgtcttg ctgatcagta tgcactgggc ggtgcgtatc agttttgagc
     9601 gtggctttat tgattacatc aagcatggta atgaacagcg attacaactg ttaagtgatg
     9661 cgcttggcga gcagtatgcg cagcatggca actggcgctt cctgcgcaac aatgatcgct
     9721 ttgtctttca gatcctgcgt tcatttgaac acgataattc ggaagataaa cccggcccgg
     9781 gtatgccacc gcacggctgg cgtacccagt tctgggtggt tgatcaaaac aacaaagtgc
     9841 tggttggtcc gcgagcgccg attccacctg acggtacacg gcgacccatt ctggtcaacg
     9901 gtgcggaagt tggcgcggtg atcgcctccc ccgttgagcg gttaacgcgc aatactgata
     9961 tcaatttcga taaacaacag cggcaaacca gctggttgat tgtcgctctg gcaacgttac
    10021 tcgcggcgct cgccaccttt ctgctggcgc gcggtttact ggcaccggta aaacgacttg
    10081 tcgatggcac gcacaaactg gcggcgggcg atttcactac ccgcgtaacg cccaccagtg
    10141 aagatgaact gggcaaactg gcgcaagact tcaaccagct tgccagcaca ctggagaaaa
    10201 accagcaaat gcggcgcgat tttatggccg atatttctca cgaactgcgt acgccattag
    10261 cggtgctgcg cggtgaactg gaagccattc aggatggcgt gcgtaaattc acgccggaga
    10321 cggtggcgtc tttacaggcg gaggtcggta cactgaccaa actggttgac gatctccatc
    10381 agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa agcaccggta gatttgatcc
    10441 cactgctgga agtggcgggc ggcgcatttc gcgaacgatt cgccagtcgt ggcctgaaac
    10501 tgcaattttc cctgccagac agtattaccg tatttggcga tcgcgaccgt ttaatgcagt
    10561 tattcaataa cttactggaa aacagcctgc gctacactga cagcggcggc agcctgcaaa
    10621 tctctgccgg gcagcgcgac aaaacggtgc gcctgacctt tgccgacagt gcgccaggtg
    10681 tcagtgacga tcagctacaa aaattgtttg aacgttttta tcgcaccgaa ggttcccgca
    10741 accgtgccag cggcggttcc gggctggggc tggcgatttg cctgaacatt gttgaagcac
    10801 ataatggtcg cattattgct gcccattcgc cttttggcgg ggtaagcatt acagtagagt
    10861 taccgctgga acgggattta cagagagaag tatgaccgag ttaccaatcg acgaaaacac
    10921 accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg cagttgctca ttgattatct
    10981 gcgtgctgcg agctatgcgc cgacgcttat cagccacggc gatcaggtac tgccgtatgt
    11041 gcgccagaca ccaccggatc tgatcctgtt agatctgatg ctccctggca ccgatggcct
    11101 gacgctgtgc cgggaaattc gtcgtttttc tgacattccg atcgtgatgg tgacggcaaa
    11161 aatcgaagag atcgatcgcc tgctggggct ggagattggc gcagatgatt atatctgtaa
    11221 gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc attttgcgcc gttgcaaacc
    11281 gcagcgcgag ttgcagcaac aggatgctga aagcccgttg attatcgacg aaggtcgttt
    11341 tcaggcttca tggcgcggta aaatgcttga cctgacacct gcggaatttc gtctgttgaa
    11401 aacgctctct catgaaccag gaaaagtgtt ctcccgcgag caattgctca atcatcttta
    11461 tgacgactac cgcgtagtaa ccgaccgcac cattgacagc cacattaaaa acctgcgccg
    11521 caagctggaa tctctcgacg ccgaacagtc atttatccgc gccgtttacg gcgtcggtta
    11581 ccgctgggaa gccgacgcct gccgcatcgt ttagttttag cgacattatt ttgttagccg
    11641 gagatggcgt tcagacccga tccggcaaca ttatcccacg catggtcagc aaactgacat
    11701 tatctctccg ccctggcagg catcagaaaa tcgctcatac tttaatcggt aaacagcacc
    11761 tttagatgct gttttcgata cacaatttca atcaaggagt cattatggct ggttggtttg
    11821 aactcagtga gagcagtgat aatcagttcc ggtttgtgct aaaagcgggc aatggtgaga
    11881 ctatcctcac cagcgagctg tatacctcaa aagcctctgc ggaaaagggc atcgcgtcgg
    11941 tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa aacggcaagt aacggcaaat
    12001 tctatttcaa tctgaaagcc gctaatcatc aaattatcgg ctccagccag atgtacgcca
    12061 ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc caatggcaca agccagacgg
    12121 tgaaagacaa tacgtaatta cgatgccggg cgtgggttgt tggcgtccgg ctttatatcc
    12181 aaataacgct tagttattaa taattaagag taaccgccgc cattaaaata gtgggcactg
    12241 acgagcactc gttgtgcttt ctttgtttac catcccatac ttatcctcct gaacatgttc
    12301 atgtgccaaa atatcttcta atactttcgt gacgctatcc ggtttatgac atacactgat
    12361 gtaagatttt tacttacagg cgggcgtaaa atggccagaa cgatgacggt tgatctcggc
    12421 gatgagttgc gtgaatttat cgagtcctta attgaatccg gcgactatcg cacccaaagc
    12481 gaagtgatcc gtgagtcact gcgattactt cgagaaaagc aagcagagtc tcgcctgcaa
    12541 gcgttacgtg acatgctcgc agaagggttg agtagtggtg aagctcagcc gtgggaaaag
    12601 gacgcgttct tacggaaggt gaaagcaggg ataaggaaat aatgtgcgta ttattaaact
    12661 catgccaaaa gccaatgagg atttagaagg catctggtat tacagctatc accatttcgg
    12721 cgaaccacaa gccgacagat atgtggaaca tctttcagac gttctccaga ttctgagtaa
    12781 caataatata ggtacgccca gacctgaatt aggcgaaggt atttttgttc ttccttttga
    12841 acgccacgtc atttatttcc tgcaatcacc aggggaaatt atcgtcattc gtatccttaa
    12901 ccaaaaccag gacgccacca gacatttaca ctggagctaa acccgccgat tgcgctacaa
    12961 tgcccgccct taaagtgggg gcactcccct aaccgcttca tcaggtgaag cggatctgac
    13021 ctgtcatcag aacgagagaa ttatgtttaa accggaactc ctttccccgg cgggaacgct
    13081 gaaaaatatg cgttacgctt tcgcttatgg cgcagatgct gtttatgcgg gccagccgcg
    13141 ttattccctg cgtgtgcgca acaacgaatt caaccacgaa aatcttcagc tcggcatcaa
    13201 tgaagcccac gcgctgggga aaaagtttta tgtcgtggtc aacattgcac cgcacaacgc
    13261 caagctgaaa acctttatcc gtgacctgaa accggtggtg gaaatggggc cggatgcgct
    13321 gattatgtcc gatccagggc tgattatgct ggtgcgtgag cacttccctg aaatgccgat
    13381 ccacctttcg gtgcaggcta acgccgtgaa ctgggcgacg gtgaaattct ggcagcaaat
    13441 gggcctgacc cgcgtgatcc tctctcgcga gctgtcgctg gaagagattg aagagatccg
    13501 caatcaggtg ccggatatgg agatcgagat cttcgttcac ggcgcgctgt gcatggccta
    13561 ctccggtcgc tgcctgctct ctggctatat caacaagcgc gacccgaacc agggcacctg
    13621 caccaacgcc tgccgctggg agtacaacgt ccaggaaggg aaagaagatg atgttggcaa
    13681 catcgtacac aagtacgagc cgattccggt gcaaaatgtt gagccgacgc tgggtatcgg
    13741 cgcaccaacc gacaaagtgt ttatgatcga agaggcccag cgtccgggcg agtatatgac
    13801 cgcgtttgaa gatgagcacg gcacttacat catgaactcg aaagatctgc gcgccatcgc
    13861 ccatgtagaa cgcctgacca aaatgggcgt gcattcgctg aaaatcgaag gtcgtaccaa
    13921 atctttctac tattgtgcac gcaccgcaca ggtttaccgc aaagctatcg atgacgccgc
    13981 tgcgggaaaa ccgttcgata ccagcctgct ggaaactctg gaaggtctag cgcatcgtgg
    14041 ctataccgaa ggtttcctgc gtcgtcatac tcacgacgat tatcagaact acgaatacgg
    14101 ttattcagtt tctgaccgcc agcagtttgt tggtgagttt accggtgagc gcaaggggga
    14161 cctcgcggcg gtagcggtga aaaataaatt ctccgttggc gacagccttg agctgatgac
    14221 gccgcaaggc aacattaatt ttacccttga gcacatggaa aacgccaaag gcgaagctat
    14281 gccgatagca ccaggcgatg gttatactgt gtggctcccg gtcccgcagg atcttgagct
    14341 caattacgcg ctgctgatgc gtaatttctc cggggaaacc acgcgtaatc cccacggtaa
    14401 gtgattaatt tcgattattt ttcccggatg gaaaattctt agaaaccgat cacatacagc
    14461 tgcatttatt aaggttatca tccgtttcgc tgaaaaacat aacccataaa atgctagctg
    14521 taccaggaac cacctcctta gcctgtgtaa tctcccttac acgggcttat tttttatgca
    14581 taagccctat ccctggtcac cgtcttccat tgaccacatc gatagaatct cccttcatag
    14641 cacgatgcct ttcacgtaac ggcatcgtgc tcgcacaggt tccggctaag cacaaccaga
    14701 acgcgcatgt ttgacgctta ccaaaaaata ttctcactct ccacatttga atgtcagacg
    14761 agcgacgcca tgtaatcctg caccttctgt cttcaggtca actatctgca tttttttgcc
    14821 ctgagtaaca cagaaatggg ctgcatcatt ttttactata ttttctgcac cagatattct
    14881 gcccctggct aaagaagctt cggcttcggt gtagtattgg ttatcgagtt tacgctgaat
    14941 attactttta tatgcaagac caaatttacc gatacttgtc tcatcattat gcacagcaca
    15001 accagacaca ataaaaatac taattaatga tatagcaact atctttttca tctcaccttc
    15061 ccccattaaa taccaacgac actctctagt gtttaaatat aataatgg
//
LOCUS       DAPGWD010000102        14991 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPGWD010000102 DAPGWD010000000
VERSION     DAPGWD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.103"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            127..369
                     /locus_tag="RHX88_003046"
     CDS             127..369
                     /locus_tag="RHX88_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231710.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            483..1121
                     /locus_tag="RHX88_003047"
     CDS             483..1121
                     /locus_tag="RHX88_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDV2231711.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLV
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1118..1855
                     /locus_tag="RHX88_003048"
     CDS             1118..1855
                     /locus_tag="RHX88_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV2231712.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1855..3951
                     /locus_tag="RHX88_003049"
     CDS             1855..3951
                     /locus_tag="RHX88_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV2231713.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3998..4276)
                     /locus_tag="RHX88_003050"
     CDS             complement(3998..4276)
                     /locus_tag="RHX88_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231714.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4491..4901
                     /gene="psiE"
                     /locus_tag="RHX88_003051"
     CDS             4491..4901
                     /gene="psiE"
                     /locus_tag="RHX88_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDV2231715.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4945..6420)
                     /gene="xylE"
                     /locus_tag="RHX88_003052"
     CDS             complement(4945..6420)
                     /gene="xylE"
                     /locus_tag="RHX88_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HDV2231716.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6792..7682)
                     /gene="malG"
                     /locus_tag="RHX88_003053"
     CDS             complement(6792..7682)
                     /gene="malG"
                     /locus_tag="RHX88_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDV2231717.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7697..9241)
                     /gene="malF"
                     /locus_tag="RHX88_003054"
     CDS             complement(7697..9241)
                     /gene="malF"
                     /locus_tag="RHX88_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDV2231718.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9395..10585)
                     /gene="malE"
                     /locus_tag="RHX88_003055"
     CDS             complement(9395..10585)
                     /gene="malE"
                     /locus_tag="RHX88_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDV2231719.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10950..12065
                     /gene="malK"
                     /locus_tag="RHX88_003056"
     CDS             10950..12065
                     /gene="malK"
                     /locus_tag="RHX88_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDV2231720.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12137..13477
                     /gene="lamB"
                     /locus_tag="RHX88_003057"
     CDS             12137..13477
                     /gene="lamB"
                     /locus_tag="RHX88_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDV2231721.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13720..14640
                     /gene="malM"
                     /locus_tag="RHX88_003058"
     CDS             13720..14640
                     /gene="malM"
                     /locus_tag="RHX88_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDV2231722.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3720 a         3846 c         3930 g         3495 t
ORIGIN      
        1 ccaattatcc taaaacccca tctctaattc cccgcgccgt aattcgcatg ctttagttgt
       61 gtatactcga tcccgcccga aatgtttttg ggtaaatctc cattcattca atgaagggaa
      121 attgttatga aaaaagttct gtatggcatt tttgccatat ctgcgcttgc ggcgacttct
      181 gcgtgggctg cacctgtaca ggtgggcgaa gcggcagggt cggcagcaac gtcggtttcg
      241 gcggggagtt cctccgcgac cagcgtcagc accgtaagct cggcggtggg tgtcgcgctc
      301 gcggcaaccg gtggcggtga tggttctaat accgggacca caacaaccac gaccaccagt
      361 acccagtaat aaagtatgta tccccaaaat aattcgagtc atcgcatctg tggcttgaag
      421 tatgaaggga tttaccataa ccacactccg gtgtggttat tctgcccctc tggagaagag
      481 tcgtgaagcg acctgcactc attcttatct gcctgctatt acaggcctgt tcagccacga
      541 ctaaagagct gggcaattca ctgtgggaca gtctgttcgg tacgccaggc gtacagctga
      601 cggatgatga tattcaaaat atgccctacg ccagccagta tatgcagctt aatggcgggc
      661 cgcagttgtt tgtagtgctg gcctttgccg aggatgggca acaaaaatgg gttactcagg
      721 atcaggccac tctcgtcaca caacatggtc gtctggtgaa gactttgctt ggcggcgaca
      781 acctggttga agtgaataac cttgccgccg acccgctgat taaaccagca caaattgttg
      841 atggtgcaac atggacgcgc acgatgggct ggaccgagta ccagcaggta cgctacgcca
      901 ccgcacgctc agtcttcaaa tgggatggca ccgataccgt caaggtcggc agcgatgaaa
      961 ccccggttcg cgtgctggac gaagaagtct ccaccgacca ggcgcgctgg cataaccgct
     1021 actggatcga cagcgaaggg caaattcgcc agtcggaaca gtatctcggc gcggattatt
     1081 tcccggtgaa aaccactctc atcaaggcgg caaaacaatg attaaacaaa ctattgtcgc
     1141 gttgcttttg agtgtgggag cgtcatcggt cttcgcggca ggaaccgtca aggtgttcag
     1201 caatggcagc agtgaggcca aaacgctgac gggtgcagag catttaatcg atctggtagg
     1261 ccaaccgcgg ctggcaaaca gctggtggcc cggtgcggtg attagcgaag agctggcaac
     1321 ggcggcagca ttgcgtcagc agcaggcgtt gctgacacgg ctggctgaac aggctgcaga
     1381 ttccagcgcc gacgatgccg ccgccattaa cgccttacgc cagcaaattc aggcgttgaa
     1441 agtgacgggc aggcaaaaaa tcaatcttga tcctgatatt gtgcgcgttg ccgaacgcgg
     1501 taacccaccg ttgcagggca actacacgct gtgggtcggg ccaccaccgt ccacggtcac
     1561 gctgttcggg cttatcagcc gtcctggcaa gcagccattc actccaggtc gcgacgtggc
     1621 gagctatctc tccgggcaga acctgcttag cggtgcggat cgcagctacg cgtgggtggt
     1681 ttacccggac ggacgcacgc aaaaagcgcc ggtggcttac tggaacaagc gtcacgtaga
     1741 gccgatgccc ggcagcatta tttatgttgg cctcgcggac tccgtctgga gtgagacgcc
     1801 tgatgccctt aacgccgaca ttcttcagac tctgacgcag cggatacccc aataatgaaa
     1861 aaaagacatc tgcttagctt actggcgctg ggcattagca cagcttgcta cggcgaaaca
     1921 tatcctgcgc ccattggtcc gtcgcagtcg gatttcggtg gcgtaggatt attacaaact
     1981 cccaccgcgc gtatggcacg ggaaggggag ttgagtctga actatcgcga taacgatcag
     2041 taccgttatt actcagcttc agtgcaactc ttcccgtggc tggaaacaac gctgcgctac
     2101 accgacgtgc gcacccggca gtacagcagc gtcgaagcgt tctctggcga tcaaacgtat
     2161 aaagataaag ccttcgatct caaactgcgt ttgtgggaag agagttactg gctgccgcaa
     2221 gtggcggttg gtgcgcggga tatcggcggt acggggctgt ttgatgcgga atatcttgtt
     2281 gccagcaaag cctgggggcc gttcgatttt acgctcggcc tgggctgggg atatctgggc
     2341 accagcggta atgtaaaaaa tccgctctgt tcagccagtg ataaatattg ctatcgcgat
     2401 aacagctaca aacaggcggg atctatcgac ggtagccaga tgttccacgg tcctgcctca
     2461 ctgtttggcg gcgtggaata ccagacgccc tggcaaccgc tgcgcctgaa actggagtat
     2521 gaaggcaata attatcagca ggattttgcc gggaagctgg agcaaaaaag taagtttaac
     2581 gtcggtgcga tttatcgcgt taccgattgg gccgacgtta accttagcta tgaacgtggc
     2641 aacaccttta tgtttggcgt cacgctgcgc accaacttta acgatctgcg cccgtcttac
     2701 aacgataacg cccgcccgca atatcaaccg cagccgcagg atgccatttt gcagcattcg
     2761 gtggtggcga atcagttaac gctgttgaaa tacaatgccg gacttgccga tccgcagatc
     2821 caggcgaaag gcgatacgct gtatgtcacc ggcgagcagg tgaaatatcg tgattcgcgc
     2881 gaagggatca tccgtgctaa tcggatcgtg atgaacgatc tgccggatgg gatcaaaacg
     2941 atccgcatta cggaaaatcg tcttaacatg ccgcaggtga cgacggaaac cgatgtcgcc
     3001 agcctgaaaa atcatctcgc cggagagccg ttgggccacg aaacgaagct ggcgcaaaaa
     3061 cgcgtcgagc cagtggttcc gcagtccacc gagcagggct ggtatatcga caaatcacgc
     3121 tttgatttcc acatcgatcc ggtgctgaac cagtcggtcg gtggcccgga aaacttttac
     3181 atgtatcagc tgggcgtgat gggaacggca gatttgtggc tgacggacca tctgctgacc
     3241 accggcagcc tgtttgcaaa tcttgccaac aactacgaca agtttaacta cactaatcct
     3301 ccgcaggact cgcacttacc gcgcgttcgt acccatgtgc gcgagtatgt gcagaacgat
     3361 gtctatgtga ataacctgca agccaactac ttccagcatc tgggcaacgg cttctacggt
     3421 caggtctacg gtggttatct cgaaaccatg tttggcggtg cgggggcaga agtgttgtat
     3481 cgcccgctgg acagtaactg ggcgtttggt ctggatgcca actacgttaa acagcgcgac
     3541 tggcgtagtg caaaagatat gatgaaattc accgactaca gcgtgaaaac cggacatctg
     3601 accgcgtact ggacgccatc tttcgctcag gatgtgttag tgaaagcgag cgtcgggcag
     3661 tatctggcgg gcgataaagg cggcacgctg gagatcgcca aacgctttga tagcggcgtg
     3721 gtggttggcg gctatgccac gatcaccaac gtttcgaaag aggagtacgg cgaaggggac
     3781 ttcaccaaag gcgtgtatgt ctcggtaccg ttggatctct tctcgtctgg cccgacacgc
     3841 agccgtgcgg cgattggctg gacgccgctg acgcgtgacg gtggtcagca acttgggcgt
     3901 aagttccagt tgtatgacat gaccagcgac cgtagcgtca atttccgcta agtcatggga
     3961 aaggtgccag ttttcgcact cactggcacc ctcattctta atgacctgtt gagccatcag
     4021 gaacgcagtt cacccccggt ttacgatctg gtgtgtaaag gcgaaaacat tctgtcttcc
     4081 tgtccgtcga tatcggttgc aggtctggtt gcctgagcga ctgggccatc attgggctgg
     4141 agacattgct ttgcggtagg tcggctttat cagcagtacc gagcggacca gcataagcag
     4201 gaagaacaga gactgataac atcaaagcag caaaataagg cttcattttt accaccttta
     4261 tcaggttacg tttcatttgt tccagaggaa cattgtcgat ttttcgcgca ttgctggtgg
     4321 ctgggaatca cctgaatggg tgattttttg gggctgtgtg ttttgccaga tgcgacgttg
     4381 atgggagatg cccacttaat caagctgaac aaaacataca caaaaaatat agatctccgt
     4441 cacatttttg cgttatacag gaagctcgcc actgtgaagg aggtactgct atgacgtcac
     4501 tctctcgtcc gcgcgtggag tttatctcca ccattttgca gaccgtactc aatcttggcc
     4561 tgttgtgcct cggcctgatt ttggttgtct tcctcggcaa agaaacggtg catctggcag
     4621 atgtgctgtt cgcgccagaa caaaccagca aatatgagct ggtagaagga ctggtggttt
     4681 actttctcta tttcgaattt atcgcgctga ttgtgaaata ctttcagtcc ggttttcact
     4741 tcccgctacg ttactttgtc tatattggga tcaccgccat tgtgcgcttg atcatcgtcg
     4801 atcataaatc gccactggat gtgttgatct actcggctgc gatcctgtta ctggtgatca
     4861 ctctgtggct gtgtaattcg aaacggctga agcgggagta aaaagacagc acgccgaact
     4921 ggcgcggcgt gctggacagg aagattacag cgtagcagtt tgttgtgttt tcttcgtttc
     4981 cggttcccag agggcttcca gctcctcaag ggttttacct ttggtttccg ggacaaattt
     5041 ccacataaat agtgctgcca gaacgcccat acacccgtaa atccagtagg agaaaccgtt
     5101 gtggaaatgg gccaccagcc aggagttttt gtccatcatc gggaaggtcc aggagacgaa
     5161 gtagttcgcc agccactggg ccgccaccgc gattgccagc gctttaccac gaatagcatt
     5221 cgggaagatt tccgacagca gtacccagca taccggaccc caggacatgg caaaggcggc
     5281 aacatagaac agcatcgaca gtagcgccac aatacccgat gcctgagtgt aaaacgcggt
     5341 accgaggcta aacataccga ttgccattcc gagtgcgccg ataatttgca gtggcttacg
     5401 accaaattta tccaccgtca taattgccag tacggtaaag gtgaggttga taactccgac
     5461 aataatggtc tgcaacagcg cgatatccgt gctggccccc agcgttttga acacttccgg
     5521 cgcgtagtac agcaccacat tgatgccgac aaattgctgg aagatggaga gcataacgcc
     5581 gattacaatc acgcccacgc caaacatcag cagacgacca ccggttttgc ggccatgatc
     5641 cagggagtgt ttaatttcct gtactgcctg agttgcaagc gtgttgccca taattttgcg
     5701 caggatactt tccgcctgtt cttgcttgcc gcgtgacatc agccagcgag gactttctgg
     5761 cacggtatac agcagcatta agaacagcag tgcagggata cattccgagg caaacatata
     5821 acgccagccg tcagtattca gccagctggc atcaccggaa cgggcaataa aatagtttac
     5881 gcagtaaact aaaagttgcc cgaaaataat cgcaaactgg ttaaatgaaa ccagttttcc
     5941 gcgaatatga gctggagcca gttccgcaat atacattggt gaaagcattg aggctaaacc
     6001 aacgccaata ccgccaataa tacgataaat aacaaattcc gggacataac ctgccagata
     6061 aatagggact gtgttgtccg ggtttataga ggtaaaacca agttctggcc aggcagaacc
     6121 tacaccagaa ataaaaaaca ggacagcagc aatcttaagt gaatcacgac ggccgaagcg
     6181 gttactgcaa taaccaccga gggcaccgcc gatgatgcaa ccaatcagag cgctggctac
     6241 gcaaaatcct aacagggagt tggcagcgga ttcacttaag ttttgtggag caacaaagac
     6301 ggtattgagt gactcaacag taccggaaat aacggcggtg tcgtagccaa ataataaacc
     6361 acctaatgta gcgactaagg taatcgaaaa tatataactg gaattatact gggtattcat
     6421 tcagacctgc cttagaccat tctgatcttt tatcagtgca ttgatgttgg aattgtcagg
     6481 cagttaagac aattatcatt ttttacaatg cgtttacgta atgtcttttc tgtgatctta
     6541 attgtgataa ttatcaaaaa ttgatgaaaa caatactatt gccgtgactc agagcacgaa
     6601 agagaattat cgtaagtggg aaaacaaata acgtaaaaat aataagttct atgatgggaa
     6661 tattaaccgg cgaacgattc agattgcaga cgaaagaaaa aaaggcgctc cgtggagcgc
     6721 cgaataatag tcacaagttg ggataacgta agttgagggt gcagcggcat aacattggca
     6781 gaacaacatc tttaaccttt cacaccacct gccgtcaggc cgttgaccag ccagcgttga
     6841 gccagcaaga agacgatggt gatcggtaat gcagacatca cggcagcggc ggcaaagtca
     6901 ccccacaggt agttttgcgg gttgaggtat tgctgcatcc ccacggccag ggtgtagcta
     6961 tttacgtcac gcagtaacag cgacgcgacc ggaacttcag taatggcagc gataaacgac
     7021 aggatgaaca ccaccgccag aatcggtact gacagcggca acaggacaag gcggaaggcc
     7081 tgccacggtg tcgcaccatc cagcgccgcc gcttcttcca gcgaactgtc gatggtttcg
     7141 aaatagcctt tgatggtcca gacatgcagc gcaatcccac ccagatacgc gaaaattacg
     7201 ccgccgtgag tattcaggcc aatgaatgga atgtactcac ccagacgatc aaacaacgca
     7261 tacaacgcga ccagtgaaag tactgccggg aacatctgga aaatcagcat tcctttcagc
     7321 agcgtcgctt tgcctggaaa gcgcatacgg gcgaaagcgt aggcgcaggt ggtggagagc
     7381 gccacaatgc caatcgcgga aatcccagcg acctttaccg agttccacag ccacagcagt
     7441 accgggaatg gcggtggcgt aatgcgacca tcagcctgtt caacgctaaa acctaacgcc
     7501 agtttccagt gatcccagga gatttgctcc gggatcaggc taccggtcgc gaagtttccc
     7561 tgacgcagcg agatagcgac gaccatcagc agcgggaaca taatcgccgc gataaaaagt
     7621 agcagtagca ggtgagtaat aaacaaacgt gctttttgcg atttcggttg gaccattgcc
     7681 atttttgtta tctcccttaa tcaaacttca ttcgcgtggc tttcaggttc actatcgcca
     7741 gcgcacccac cagcaggaag atcagcgtgg caattgctgc cgccagaccg aagtcctgac
     7801 ccccgccgcc ttcaaaagcg atgcggtaag tgtagttaac aagcaggtcg gtataaccgg
     7861 ctggcgtggt cgtgccaaga cgatccgggc cgccgttggt taacagctga atcagcacga
     7921 agttgttaaa gttaaaggcg aagctggcga tcatcagcgg cgtcagcggt ttaatcagca
     7981 gcggcagcgt aatcttaaag aagttctgga acggacctgc gccatccatt gctgaggctt
     8041 catacaaatc gtccggaatc gctttcagca agcccatgca gaggatcatc atgtacggat
     8101 aacccagcca ggtattgacg ataatcagca tcgttcgggc agtggtcgga tcgctgaacc
     8161 aggcgggctt cacgccaaac agcgcgctca acatcatgtt gatttcaccg aagctctggt
     8221 taaacaaccc tttgaaaatc aagattgaaa tgaatgatgg caccgcgtag ggcagaatca
     8281 gcaggacgcg atataccgct ttgccacgca acgcttccca ctgcaccaga cacgccagaa
     8341 ccatgccgac cgccaccgtt aaaaagacgg tgatcagtga aaacaccacg gtccagacga
     8401 aaatggcgag gaacggtttc tgaatgcctt cgtcggtaaa gacgcgggta aagtttttcc
     8461 agccggtggt cacggtgtaa ccggggctta gcttttcatc accccagttg ccgtcggcgg
     8521 taatggactg gtaaaagcca atttggttat tcggacgata tttcacgccg ctctgattat
     8581 tcgtcaacgt gccgtcaccg tcgagtgtgt agagcggctg cgtgccagaa aactggcgca
     8641 gggagctcat catcactttg ttgccatccg gcagaatggc ggtaatgtca ctcagcgcct
     8701 gacgattctg ggtaatcacg cgtagattcg cgcgttcgcc ttcgggctgg gcggtcgttt
     8761 ctttcagttg cagtttttgc tcgccgccaa atttaaaagc gtcggaaagg taatttttgc
     8821 cggtttcgcc gtcactgagc gccagttgcc actcatcgcc cgccgggtaa agaccaaagt
     8881 tataggtttt gcctgcttgc caggagcgat ctaacaacac ttcctgtgca cgttcaaaag
     8941 tcagctggtt agtgctgctg tagttggtga aggcaatggc gatggtgcag accagaggga
     9001 agaggacgaa taatcccatt ccagccattc ccgggtaaac atagcgccag gcgtaggctt
     9061 tacgattggc gaaaatatac agccccgctg aactcaatat cagcgtggta atggcgaaca
     9121 ggtattcccc ttgtgcgtac attaaaacaa caaggtaacc caccagcagg ccgagcagac
     9181 ctagcactga ccatttcagc gcgtcgcttt gccaccaatg tttcttttta atgacatcca
     9241 tggggttctt cctcattcca ggacggataa ggctttcacg ccttatccga caacaactgc
     9301 ctgatgcgac actgatgcgt cttatcaggc ctacatgcgt ttcagttttg taggccggac
     9361 aaggcgttca cgccgcatcc ggcatttcac agcattactt ggtgatacga gtctgcgcgt
     9421 ctttcagggc ttcatcgaca gtctgacgac cgctggcggc gttgatcacc gctgtacgca
     9481 cggcatacca gaaagcggac atctgcggga tgttcggcat gatttcacct ttctgggcgt
     9541 tttccatggt ggcggcaata cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg
     9601 ccactgcacc cagcggtttg tctttattga ccgcttccag accttcgtca gtcagcagat
     9661 agttttcgag gaactctttc gccagctctt tgttcggact ggcggcgtta ataccggcgc
     9721 tcagcacgcc aacgaacggt ttagatggtt gacccttgaa ggtcggcagt accgttacac
     9781 cataattcac tttgctggtg tcgatgttgg accatgccca cgggccgttg atggtcatcg
     9841 ctgtttcgcc tttattaaag gcagcttctg cgatggagta atcggtgtct gcattcatgt
     9901 gtttgttttt aataaggtca accaggaagg tcagacccgc tttcgcgcca gcgttatcca
     9961 cgcccacgtc tttaatatcg tacttgccgt tttcatactt gaacgcataa cccccgtcag
    10021 cagcaatcag cggccaggtg aagtacggtt cttgcaggtt gaacatcagc gcgctcttac
    10081 ctttcgcttt cagttcttta tccagcgccg ggatctcttc ccaggttttt ggtgggttcg
    10141 gcagcagatc tttgttataa atcagcgata acgcttcaac agcgatcggg taagcaatca
    10201 gcttgccgtt gtaacgtacg gcatcccagg taaacggata cagcttgtcc tggaacgctt
    10261 tgtccggggt gatttcagcc aacaggccag attgagcgta gccaccaaag cggtcgtgtg
    10321 cccagaagat aatgtcaggg ccatcgccag ttgccgcaac ctgagggaat ttctcttcca
    10381 gtttatccgg atgctcaacg gtgactttaa ttccggtatc tttctcgaat ttcttaccga
    10441 cttcagcgag accgttatag cctttatcgc cgttaatcca gattaccagt ttaccttctt
    10501 cgattttggc gagagccgag gcggaaaaca tcatcgtcgt taatgcggat aatgcgagga
    10561 tgcgtgcacc tgtttttatt ttcataatct atggtccttg ttggtgaagt gctcgtgaaa
    10621 acacctaaac ggactctagt ttctttatac ggcaacctct ttccatcctc cttgccccta
    10681 cgccccaccg ttgctttgtg tgatctctgt tacagaattg gcggtaatgt ggagatgcgc
    10741 acataaaatc gccacgattt ttgcaagcaa catcacgaaa ttccttacat gacctcggtt
    10801 tagttcacag aagccgtgtt ctcatcctcc cgcctcctcc cccataaaaa agccaggggg
    10861 tggaggattt aagccatctc ctgatgacgc atagtcagcc catcataaat gttgctgtcg
    10921 atgacaggtt gttacaaagg gagaagggca tggcgagcgt acagctgcaa aatgtaacga
    10981 aagcctgggg cgaggtcgtg gtatcgaaag atatcaatct cgatatccat gaaggtgaat
    11041 tcgtggtgtt tgtcggaccg tctggctgcg gtaaatcgac tttactgcgc atgattgccg
    11101 ggcttgagac gatcaccagc ggcgacctgt tcatcggtga gaaacggatg aatgacactc
    11161 cgccagcaga acgcggcgtt ggtatggtgt ttcagtctta cgcgctctat ccccacctgt
    11221 cagtagcaga aaatatgtca tttggcctga aactggcagg cgcaaaaaaa gaggtgatta
    11281 accaacgcgt taaccaggtg gcggaagtgc tgcaactggc gcatttgctg gatcgcaaac
    11341 cgaaagcgct ctctggcggt cagcgtcagc gtgtggcgat tggccgtacg ctggtggccg
    11401 agccaagcgt atttttgctc gatgaaccgc tctccaacct cgatgctgca ctgcgtgtgc
    11461 aaatgcgtat cgaaatctcc cgtctgcata aacgcctggg ccgcacaatg atttacgtca
    11521 cccacgatca ggtcgaagcg atgacgctgg ccgacaaaat cgtggtgctg gacgccggtc
    11581 gcgtggcgca ggttgggaaa ccgctggagc tgtaccacta tccggcagac cgttttgtcg
    11641 ccggatttat cggttcgcca aagatgaact tcctgccggt aaaagtgacc gccaccgcaa
    11701 tcgatcaagt gcaggtggag ctgccgatgc caaatcgtca gcaagtctgg ctgccagttg
    11761 aaagccgtga tgtccaggtt ggagccaata tgtcgctggg tattcgcccg gaacatctac
    11821 tgccgagtga tatcgctgac gtcatccttg agggtgaagt tcaggtcgtc gagcaactcg
    11881 gcaacgaaac ccaaatccat atccagatcc cttccattcg tcaaaacctg gtgtaccgcc
    11941 agaacgacgt ggtgttggta gaagaaggtg ccacattcgc tatcggcctg ccgccagagc
    12001 gttgccatct gttccgtgag gatggcactg catgtcgtcg actgcataag gagccgggcg
    12061 tttaagcacc ccacaaaaca cacaaagcct gtcacaggtg atgtgaaaaa agaaaagcaa
    12121 tgactcagga gatagaatga tgattactct gcgcaaactt cctctggcgg ttgccgtcgc
    12181 agcgggcgta atgtctgctc aggcaatggc tgttgatttc cacggctatg cacgttccgg
    12241 tattggctgg acaggtagcg gcggtgaaca acagtgtttc cagactaccg gtgctcaaag
    12301 taaataccgt cttggcaacg aatgtgaaac ttatgctgaa ttaaaattgg gtcaggaagt
    12361 gtggaaagag ggcgataaga gcttctattt cgacactaac gtggcctatt ccgtcgcgca
    12421 acagaatgac tgggaagcta ccgatccggc cttccgtgaa gcaaacgtgc agggtaaaaa
    12481 cctgatcgaa tggctgccag gttccaccat ctgggcaggt aagcgcttct accaacgtca
    12541 tgacgttcat atgatcgact tctactactg ggatatttct ggtcctggtg ccggtctgga
    12601 aaacatcgat gttggcttcg gtaaactctc tctggcagca acccgctcct ctgaagctgg
    12661 tggttcttcc tcttttgcca gcaacaatat ttatgactat accaacgaaa ccgcgaacga
    12721 cgttttcgat gtgcgtttag cgcagatgga aatcaacccg ggcggcacat tagaactggg
    12781 tgtcgactac ggtcgtgcca acctgcgtga taactatcgt ctggttgatg gcgcatcgaa
    12841 agacggctgg ttattcactg ctgaacatac tcagagtgtc ctgaagggct ttaacaagtt
    12901 tgttgttcag tacgctactg actcgatgac ctcgcagggt aaaggtctgt cgcagggttc
    12961 tggcgtcgcg tttgataacg aaaaatttgc ctacaatatc aacaacaacg gtcacatgct
    13021 gcgtatcctc gaccacggtg cgatctccat gggcgacaac tgggacatga tgtacgtggg
    13081 tatgtaccag gatatcaact gggataacga caacggcacc aagtggtgga ccgttggtat
    13141 tcgcccgatg tacaagtgga cgccaatcat gagcaccgtg atggaaatcg gctacgacaa
    13201 cgtcgaatcc cagcgcaccg gcgacaagaa caatcagtac aaaattaccc tcgcacaaca
    13261 atggcaggct ggcgacagca tctggtcacg cccggctatt cgtgtcttcg caacctacgc
    13321 caagtgggat gagaaatggg gttatgacta caacggcgat agcaaggtta acccgaacta
    13381 cggcaaagcc gttcctgctg atttcaacgg cggcagcttc ggtcgtggcg acagcgacga
    13441 gtggaccttc ggtgcccaga tggaaatctg gtggtaatag caaaacctgg gccggataag
    13501 gcgtttacgc cgcattcggc aaccaacgcc tgatgcgacg cttgcgcgtc ttatcaggcc
    13561 tacaacggct gtcaaatgta ggccggataa ggcgtttacg ccgcatccgg cataaaaaca
    13621 ggttgtcatt atctgaaagg ggcgaaagcc cctctgatta tcgggtttag cgcgctattg
    13681 cctggctacc gctgagctcc agattttgag gtgaaaacaa tgaaaatgaa taaaagtctc
    13741 atcgccctct gtttatcagc agggttactg gcaagcgcgc ctggaatcag ccttgccgat
    13801 gttaactacg taccgcaaaa caccagcgac gcgccagcca ttccatctgc cgcgctgcaa
    13861 caactcacct ggacaccggt cgatcaatct aaaacccaga ccacccaact ggcgaccggc
    13921 ggccaacaac tgaacgttcc cggcatcagt ggtccggttg ctgcgtacag cgtcccggca
    13981 aacattggcg aactgaccct gacgctgacc agcgaagtga acaaacaaac cagcgttttt
    14041 gcgccgaacg tgctgattct tgatcagaac atgaccccat cagccttctt ccccagcagt
    14101 tatttcacct accaggaacc aggcgtgatg agtgcagatc ggctggaagg cgttatgcgc
    14161 ctgacaccgg cgttggggca gcaaaaactt tatgttctgg tctttaccac ggaaaaagat
    14221 ctccagcaga cgacccaact gctcgacccg gctaaagcct atgccaaggg cgtcggtaac
    14281 tcgatcccgg atatccccga tccggttgct cgtcatacca ccgatggctt actgaaactg
    14341 aaagtgaaaa cgaactccag ctccagcgtg ttggtaggac ctttatttgg ttcttccgct
    14401 ccagctccgg ttacggtagg taacacggcg gcaccagctg tggctgcacc cgctccggca
    14461 ccggtgaaga aaagcgagcc gatgctcaac gacacggaaa gttattttaa taccgcgatc
    14521 aaaaacgctg tcgcgaaagg tgatgttgat aaggcgttaa aactgcttga tgaagctgaa
    14581 cgcctgggat cgacatctgc ccgttccacc tttatcagca gtgtaaaagg caaggggtaa
    14641 ttacgcccca cagtgctgat tttgcaacaa ctggtgcgtc tcctggcgca ccttttttta
    14701 tgcttccttc ctgggatatg agcgattttt tatagtaact cacttcttct tcactaagaa
    14761 tatccattat ctcaatgcct tatcagagat tcttttcctt tcgccggtag tgtctggaca
    14821 ttcaggctac ttttccaggt tattttattt ctgttatgca gaggttttat gataagtcat
    14881 atcctaaatt ctggcggcaa taactctttg atgaaacatg atgtggtgca aggaaataat
    14941 atagtagatc ttgatttact acgtaatttc aatggggtgc caggtttaaa c
//
LOCUS       DAPGWD010000103        14757 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPGWD010000103 DAPGWD010000000
VERSION     DAPGWD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.104"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..749
                     /locus_tag="RHX88_003059"
     CDS             81..749
                     /locus_tag="RHX88_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDV2231723.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(767..1268)
                     /gene="tnpA"
                     /locus_tag="RHX88_003060"
                     /pseudo
     CDS             complement(767..1268)
                     /gene="tnpA"
                     /locus_tag="RHX88_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1276..2484
                     /locus_tag="RHX88_003061"
     CDS             1276..2484
                     /locus_tag="RHX88_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2231724.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(2524..3738)
                     /gene="ydcO"
                     /locus_tag="RHX88_003062"
     CDS             complement(2524..3738)
                     /gene="ydcO"
                     /locus_tag="RHX88_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDV2231725.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            3791..4327
                     /gene="sutR"
                     /locus_tag="RHX88_003063"
     CDS             3791..4327
                     /gene="sutR"
                     /locus_tag="RHX88_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDV2231726.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            4400..6361
                     /gene="rlhA"
                     /locus_tag="RHX88_003064"
     CDS             4400..6361
                     /gene="rlhA"
                     /locus_tag="RHX88_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDV2231727.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(6453..6683)
                     /locus_tag="RHX88_003065"
     CDS             complement(6453..6683)
                     /locus_tag="RHX88_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDV2231728.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            6905..7081
                     /gene="hicA"
                     /locus_tag="RHX88_003066"
     CDS             6905..7081
                     /gene="hicA"
                     /locus_tag="RHX88_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDV2231729.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            7127..7543
                     /gene="hicB"
                     /locus_tag="RHX88_003067"
     CDS             7127..7543
                     /gene="hicB"
                     /locus_tag="RHX88_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDV2231730.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            7622..9028
                     /locus_tag="RHX88_003068"
     CDS             7622..9028
                     /locus_tag="RHX88_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV2231731.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            9273..10418
                     /locus_tag="RHX88_003069"
     CDS             9273..10418
                     /locus_tag="RHX88_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2231732.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            10436..11449
                     /locus_tag="RHX88_003070"
     CDS             10436..11449
                     /locus_tag="RHX88_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2231733.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            11450..12391
                     /locus_tag="RHX88_003071"
     CDS             11450..12391
                     /locus_tag="RHX88_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2231734.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            12381..13175
                     /locus_tag="RHX88_003072"
     CDS             12381..13175
                     /locus_tag="RHX88_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2231735.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAARRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            13197..14621
                     /gene="patD"
                     /locus_tag="RHX88_003073"
     CDS             13197..14621
                     /gene="patD"
                     /locus_tag="RHX88_003073"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDV2231736.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3659 a         3597 c         3905 g         3596 t
ORIGIN      
        1 aacacttttt ttgccacaac acgaagcggc gctttttgct ataacttaaa aagtaatata
       61 atcatctcag gaaactattc atgcgtacca catcatttgc gaaagttgca gctttatgcg
      121 gcttgttggc tctgtctggt tgtgcctcta aaatcaccca gccagataaa tattctggtt
      181 ttttaaacaa ttactctgat ttaaaagaaa caacctcagc aacaggtaaa cctgttttac
      241 gttgggtaga cccgagtttt gatcaaagca aatatgacag catcgtctgg aacccaatca
      301 cttattatcc ggtgccaaaa ccttctaccc aggtagggca gaaagttctg gataaaattt
      361 tgaactatac caacaccgaa atgaaagaag cgatagcgca gcgtaaacca gtggttacca
      421 ccgctgggcc gcgtagtctg attttccgtg gggccattac cggtgtagat accagcaaag
      481 aagggctgca attctatgaa gtggttcctg ttgcattagt ggttgcgggg acgcaaatgg
      541 ctacaggcca ccgtaccatg gatactcgcc tctattttga aggtgagctg attgatgcag
      601 cgactaataa accggttatc aaagtcgttc gtcagggcga aggtaaagac ctgaataacg
      661 aaagtacgcc aatggctttc gaaaatatta aacaagttat tgatgacatg gcgaccgatg
      721 ccaccatgtt tgacgttaac aaaaagtagt tccagacgcg ccatcgttag atggcgcttt
      781 ttatccggtg tgccgtaaaa ccccatcctt cagggcgggg atataaggtg cggttttcca
      841 cctgaccagg tgtttgctgt tgttcaatgt attggcggat gacggatatt ggcgcacctc
      901 cgcagctact ggcaaaatag ccaggactcc acaaaacgcc tttgtagtaa tacctgactg
      961 caatatctgg tcgatctcgt cgcagtaacc tacccgatac gcctttgagg ctgtttacca
     1021 gactggatat cgccagtttg ggaggttagt taattaacaa atggacgtga tctggttcgc
     1081 catccatttc aaccagttca gcttcaaaat aagcacatac atttgaaaag taagtgcgta
     1141 gtttttctgt cgcgtcaaaa aatctggcgt cggtatctgg tgacaaagac caggtgaaca
     1201 tgcatcagga aaacacaatg ccttccacgc cggatatcgg tttctttatt cacagaccaa
     1261 agtatcattt gacctgtgaa acgattacag gcatttaaat tccagttaag accaggtggt
     1321 caacaggagt gtgaaatgag gcgcttcgca ggcgcatgtc gtttcgtttt caatcgtgct
     1381 ctggcacgtc agaatgagaa tcatgaggcc ggtaataaat acatccctta cgggaaaatg
     1441 gcttcctggc tggttgagtg gaaaaatgcc actgaaacgc aatggcttaa agattctccc
     1501 tcacagccat tgcaacagtc actgaaagac cttgagcggg cttacaaaaa cttcttccgg
     1561 aagcgggctg cttttccccg attcaaaaag cggggacaga atgatgcatt ccgctacccg
     1621 cagggcgtta agctcgatca ggaaaacagc cgtatttttc tgccgaaact gggctggatg
     1681 cgctaccgga acagccgtca ggtcacgggt gttgtgaaaa atgtcactgt cagccagtcc
     1741 tgcggtaagt ggtacatcag tattcagaca gaaagtgaag tatccactcc ggctcaccct
     1801 tcagcatcaa tggtcgggct ggatgctggc gtggctaaac tcgccacgct gtcagatggc
     1861 acagtctttg agcctgtaaa cagttttcag aaaaaccaga agacgctggc gagacttcag
     1921 cgacagttaa gccgcaaggt caaattcagc aacaactggc agaagcagaa acgcaaaata
     1981 cagcgactgc attcctgtat cgcaaatatc cgcagggact accttcacaa agtcacaacg
     2041 gccgtcagca aaaaccacgc aatgatagtc attgaggatt tgaaggtcag caacatgtca
     2101 aagtcagcag cgggtacggt cagccagccg gggcgcaatg tccgggcaaa atcaggttta
     2161 aaccgttcga tactggatca gggctggtat gaaatgcacc gccagcttga gtacaagcag
     2221 ctctggcgtg gcggtcaggt gcttgctgtt ccgccagcgt atacaagcca gcgttgcgcg
     2281 tactgtggtc atacagcgaa agagaaccgc ctgtcacaaa gtaaattcag atgccaggta
     2341 tgtggatata cagcgaacgc cgatgtaaat ggcgctcgta acattttagc ggcggggcac
     2401 gccgttcttg cctgtggaga gatggtgcag tcaggccgcc cgttgaagca ggaacccacc
     2461 gaaatgattc aggcgacagc ctgaacgtag cagggatcca cgtccttcag ggcgtggagg
     2521 atgtcaatat cggtttctgt cagcgattaa attcaacacc acgtaacaaa cgcctccggc
     2581 aattaatccc cagaacgcag aaccaatccc gaccagcgtc aatccacttg ccgttaccag
     2641 aaatgccacc accgccgcgt cgcgctcacg ctcattatgc agcgcctgat acaaactgcc
     2701 gccaatggta ctcaacagcg ccagacctgc cagcatctgg atccaactta cgggcagggc
     2761 agccatcatc ccggtaatgg cactaccaaa cagacctgcg agcaaataga aaatgcctgc
     2821 aacggcagct gccagccaac gttgatcttt atccggatgc gcttccgggc tttggcaaat
     2881 agccgcggtg attgccgcaa taccgacgga ataaacgccg aaaggggaaa aaaccagtgc
     2941 cagcaatcca gtaaatacaa ttaatggcga aacaggagcc gaatatccag ctgctttcat
     3001 tgctgcgata cccggtgcgt tttgcgatgc catcgtcacc agaaaaaggg ggagtgcaac
     3061 gctcaggctg tgagcaaacg aaaaatcagg ggtaatataa gtggggagaa cgggtttaaa
     3121 gacaacatca gttgtgacaa cgtcaccttg cgcgatgacg atcacgatcc caataatcat
     3181 cgcggcaatt accgcatagc gcggcgcaac ggccttggtt gccagccata ccagcaacat
     3241 acttccacac aacgtaaatt gaccgtccag actggcaaac gcctgtaaac caaagcgtaa
     3301 taaaatcccg gcaagcattg ccgccgcaag cgagtgcgga ataatgcgca tcagacgagc
     3361 aaagagtccc gttatgccgc agaggactat taacgcgttg gtgacaataa aaacgccgat
     3421 ggcttcgtta agtgttagtc cctgcaatcc ggtgaccaac aaagccgcgc caggcgttga
     3481 ccatgcggtg agaacaggta cgcgatacca taatgtcaga gtcagcgtac tgacgcccat
     3541 tgccagcccc agcgccgtca tccagccaga gatttgtgca gtggtggctc cggcgacaat
     3601 cgctgcttgc cagattattg ccgctgaact ggcgtagcca attaataccg ccagaaaccc
     3661 cgccagtagc gtgggtggag gaatagaaaa cagacgcata gtcacctcgt gcgttatagc
     3721 gtacaggcac cgtagcattt gtccgttata acgcacaagt gataaacttc cgttttgccg
     3781 gaggagtcgc atggaaaatc tcgctcgctt tttatccacc acacttaaac aactacgcca
     3841 gcagcgcggc tggagtcttt cgcgactggc agaagcgacg ggcgtttcta aagcaatgtt
     3901 agggcagatt gagcgtaatg agtccagtcc gacggtcgcg acattatgga aaattgccac
     3961 cgggttgaac gtaccatttt caacatttat ttctccgccg cagtccgcta cgccttcagt
     4021 ttatgatcca caacagcagg cgatggtgat cacttcgctg tttccttatg atccacaact
     4081 ctgcttcgaa cacttctcga ttcagatggc atctggtgca ataagcgaat cgacgcccca
     4141 tgagaagggg gtaatcgaac atgtcgtcgt cattgatgga caacttgatc tgtgcgttga
     4201 tggggaatgg cagaccctta attgtgggga aggcgttcga tttgctgcag acgtcacgca
     4261 tatctatcgt aatggtgggg agcaaaccgt acattttcat tccctcatcc attacccgcg
     4321 cagttaagcg ggaaaactat ttcgcaacgt cgcgcttgtg gctaaaatag ccgccatttt
     4381 tcagctactg gataagaatg tgaccgtatc ttctcatcga cttgaactgt taagcccggc
     4441 acgcgatgcc gccattgccc gcgaagctat tttgcacggt gccgatgctg tttatatcgg
     4501 cggccctggt tttggtgccc gtcataatgc cagtaatagc ctgaaagata ttgccgagct
     4561 ggtgccgttt gcccatcgtt atggtgcaaa aattttcgtc acgcttaaca ccattttgca
     4621 tgatgatgag ctggaacccg cgcaacggct gattaccgac ctctaccaga ccggtgtcga
     4681 tgcgctgatt gttcaggata tggggattct ggaacttgat attccgccga ttgaactgca
     4741 cgccagtacg cagtgcgaca ttcgtacagt agaaaaagcg aagttcctct ctgatgttgg
     4801 cttcacgcag attgtgctgg cgcgagagct gaatcttgag cagatccgcg cgattcacca
     4861 ggccacggac gcgaccattg aattctttat tcatggggca ctgtgcgtgg cctattcggg
     4921 tcagtgctac atttctcatg cgcaaacagg gcgtagcgcc aaccgtggtg attgctcgca
     4981 ggcgtgccgt ttgccataca cattgaaaga cgatcagggg cgggtggttt cctatgaaaa
     5041 acatctgctg tcgatgaaag ataacgatca gactgccaac ctcggcgcgc tgattgatgc
     5101 tggcgtacgc tccttcaaga ttgaagggcg ttacaaagat atgagctacg tgaagaatat
     5161 caccgcccat tatcgccaga tgcttgatgc cattattgaa gaacgtggcg atctggcgcg
     5221 cgcttcatca ggtcgtactg aacatttctt tgttccatcg acggaaaaga ctttccaccg
     5281 tggtagcaca gattattttg tgaatgcccg taaaggcgat attggcgcgt tcgattcgcc
     5341 gaaatttatc ggcctgccgg taggcgaagt attgaaagtg gcgaaagatc atctcgatgt
     5401 tgccgtcacc gagccactgg caaatggtga tggcctgaac gtgatgatta aacgtgaagt
     5461 cgtcggtttt cgtgccaata cggtcgagaa aaccggagaa aatcagtacc gcgtctggcc
     5521 caatgaaatg ccagcagatt tgcacaaaat tcgtccacat cacccactaa accgtaatct
     5581 tgatcataac tggcagcagg cactgacaaa aacctccagc gaacgtcggg tggcggtaga
     5641 cattgaactg ggcggctggc aggaacaact gattctgacc ctcaccagtg aagagggtgt
     5701 cagcatcacg catacgctgg acgggcagtt cgacgaagcc aataacgccg aaaaagcaat
     5761 gaacaatctg aaggatggtc tggcaaaact ggggcaaacc ctctattacg cccgcgatgt
     5821 gcaaattaat ttgccggggg cgctgtttgt accaaacagt ctgttaaacc agttccgccg
     5881 tgaagctgct gacatgctgg atgctgcgcg tcttgccagt taccagcgcg gcagccgtaa
     5941 accggttgct gatcctgcgc cggtttatcc gcaaacgcat ctgagtttcc tcgcgaacgt
     6001 atacaaccag aaagcgcgtg aattttatca tcgctatggt gtgcagctga ttgacgcggc
     6061 gtatgaagca catgaagaga agggcgaagt cccggtgatg atcaccaagc attgtctgcg
     6121 ctttgccttt aatctgtgcc cgaaacaggc gaaaggcaat atcaaaagct ggaaggcgac
     6181 gccaatgcaa ctggttaacg gcgatgaagt attaacgcta aagtttgatt gccgcccatg
     6241 cgagatgcac gtcattggca aaatcaaaaa tcacatactg aaaatgccgt taccgggaag
     6301 cgtagtggca tccgtaagtc cggatgagct gctgaaaaca ttgcctaagc gaaaagggta
     6361 aaacgccagt gttctggtta ctcacaactc attgaatctg catgatattg cctgccgggt
     6421 aaggcgtcac gccgcatccg gcaacaaatg actcaacgtt gtcccgcttc tggtttgcgc
     6481 gatttttgcc agtaaaaatg ctcgcgcaaa ccttccgccg actcttccgc cacagcacgc
     6541 aattcatcgc tgtccgcttc atgacgcagc tgatgatcca cattctttac ccacacaaat
     6601 tcatgtcctt tgtgccctgc catgagttgt cctgagaaca gcgcacacgt taataagaca
     6661 accgataacg ccttcgtaaa catcctgcca cctttttctt accttttgcc gctatgatgc
     6721 cgatcgtttc ttgaggttat tattcagttt tgcaaattag cgcaaagaaa ttctggaatc
     6781 ttccttcctg attttgcatt gcattctgcc gttgcggcga tttagtgcta ttttcgagca
     6841 aattacacac ggaggtaaac actaatgttt actttgttga tatactcagc ggcagggagg
     6901 cgatgtgaaa caaagcgagt tcagacgttg gctcgaatct cagggcgtcg atgtagcgaa
     6961 tggcagcaac catttgaaac tcaggtttca tgggaggcgc agtgtcatgc cgcgtcaccc
     7021 ctgcgatgag attaaagaac cattgcgtaa agcaatcctg aaacaactcg gtttgagtta
     7081 atcgccaatt aaaaaggtta atgacatgcg agggacagtc gaaattatgc gttatcccgt
     7141 cactcttaca cccgcgccgg aaggcggtta tatggtttct tttgtggata tccctgaagc
     7201 gttgacgcag ggcgaaactg tcgctgaagc gatggaagcg gcaaaagatg ctttactgac
     7261 cgcatttgat ttttattttg aagataacga gcttatcccg ttaccttcgc cattaaatag
     7321 tcatgatcac tttattgaag tacctttgag cgtcgcctct aaagtattgc tgttaaatgc
     7381 ttttttacag tcagaaatca ctcagcaaga gttagccagg cgaattggca aacctaaaca
     7441 ggagattact cgcctattta acttgcacca tgcgacaaaa atcgacgccg tccagcttgc
     7501 ggcaaaggcg cttggcaaag agttatcgct ggtgatggtt taattacagt taacgaaaag
     7561 ttgtcatttt taacaactga tatagactgc cgaatcatct gcacataatt acgattcgat
     7621 aatgaaaaaa taccagcagc ttgcagaaca attacgcgag cagattgcgt cgggtatctg
     7681 gcaacccggc gatcgtttgc cttcgttgcg tgaccaggtg gcgctttcag gcatgagctt
     7741 tatgactgtc agccatgcct atcagttgct cgaaagtcag ggatatatta tcgcacgacc
     7801 gcagtcgggt tattacgttg cgccacaggc aataaaaatg ccgaaagcgc cagtcattcc
     7861 agtcactcga gatgaagcag tcgatatcaa cacttatatt tttgatatgt tgcaggccag
     7921 tcgcgatccg tcggtcgttc cgtttgcctc ggcctttccc gacccgcgac ttttccccct
     7981 ccaacaacta aaccgctcgc tggcgcaggt aagcaaaacc gccacggcga tgagcgtgat
     8041 tgaaaactta ccgccaggaa acgcagaact gcgtcaggct attgctcgtc gctatgcctt
     8101 acagggcatc accatttctc ctgatgaaat tgtcattact gccggggcgt tagaggcatt
     8161 aaacctcagt ttgcaagcgg taactgaacc gggcgattgg gtgatagtag agaatccttg
     8221 tttctatggc gcgttgcagg cgctggaacg gctacggctg aaagcgttat cggtggcgac
     8281 ggatgttaaa gaagggatcg atcttcaggc gttggaactg gcgttgcagg agtatccggt
     8341 gaaagcgtgc tggctgatga ctaatagcca gaatccactc ggatttacct taacgccgca
     8401 aaaaaaagca caactggtgg cgttactcaa tcagtacaac gtaacgctga ttgaagatga
     8461 cgtttacagc gaactttatt ttggacggga aaaaccgctg cctgcgaaag cgtgggatcg
     8521 ccacgatggc gttttgcatt gctcttcgtt ttcgaaatgt ctggtgcctg gttttcgtat
     8581 tggttgggtc gccgccggaa aacatgcacg taaaattcaa cgcttgcagt tgatgagtac
     8641 gctttccacc agctcaccga tgcagcttgc gctggtggat tacctttcca cgcgccgata
     8701 cgacgcccat cttcgtcgcc tgcgtcgcca gcttgcggaa cgtaaacaac gtgcctggca
     8761 ggcactgctg cgttatctgc ctgcggaagt taaaattcat catagtgaca gcggttattt
     8821 tctctggctg gagctccccg agccgttaga tgccggcgaa ttaagcctgg cggcactgac
     8881 gcatcatatc agtattgcgc cgggtaaaat gttttctacc ggtgaaaact ggtcacgttt
     8941 ttttcgtttt aataccgcgt ggcagtgggg agaacgtgaa gaacaggcgg taaaacaatt
     9001 aggcaaactt attcaagaac ggctgtaata gcgtttaatt taattcctct tagattgggt
     9061 aatatgaatt tcgaatagca gtcatatttc ctaacccctt gtctatactc cagaagataa
     9121 ccttacagac ggcataatgc gcggtagctc acaacctgat taaattttct caggggcgaa
     9181 ggtgtgcctg caagccgccg tctatggtta aacaaggaga tatttttacg gcacggcggc
     9241 tgaacaatta attacgacag gagtaagacc ttatgagcaa gacatttgcc cgcagcagcc
     9301 tgtgtgcgct caccatgaca ataatgaccg ctcacgccgc cgaaccacct accaatttag
     9361 ataaaccgga agggcgactg gatattatcg cctggccggg atacatcgaa cgcggacaaa
     9421 ctgataaaca atacgactgg gtaacgcaat ttgaaaaaga gacaggctgc gcggtgaatg
     9481 tgaaaaccgc cgcgacttcc gatgaaatgg tcagtctgat gaccaaaggg ggttacgatc
     9541 tggttacggc atccggcgat gcctcgctgc gtttgattat gggcaaacgc gtgcagccga
     9601 ttaataccgc cttgattccc aactggaaaa cgctcgatcc gcgcgtggtt aaaggcgact
     9661 ggtttaatgt tggcggcaaa gtttacggca caccttacca atgggggccg aacctgctga
     9721 tgtacaacac taaaaccttc ccgacgccgc cggatagctg gcaagtggtt tttgttgagc
     9781 aaaatctgcc ggacggcaag agcaataaag gccgcgttca ggcttatgat ggccctatct
     9841 acattgcgga cgctgcgttg ttcgttaaag ccactcagcc gcagttgggc atcagcgatc
     9901 cgtatcaact caccgaagaa cagtaccagg cggtgctgaa agtgctgcgc gctcaacaca
     9961 gtttgatcca tcgctactgg catgacacta ccgtgcaaat gagcgatttc aaaaacgagg
    10021 gtgtggttgc ttccagtgcc tggccctatc aggccaacgc cctgaaagcc gaaggccagc
    10081 ctgttgctac cgttttcccg aaggagggtg ttaccggttg ggctgatacc accatgctgc
    10141 atagcgaagc gaaacatccg gtttgcgcct acaaatggat gaactggtca ttaacgccaa
    10201 aagtgcaggg cgatgtggcg gcctggtttg gctcgttacc ggtagtgccg gaagggtgta
    10261 aagccagtcc gttattaggc gaaaaaggtt gtgaaaccaa cggttttaac tatttcgaca
    10321 aaatcgcctt ctggaaaacg cctatagcag aagggggcaa gtttgttccc tacagtcgct
    10381 ggacgcagga ttacattgcc attatgggcg gtcgctaact tcgctggggt gctttatgac
    10441 gtacgcagtg gagtttgaca acgtctcgcg gttgtacggt gacgtgcgcg cagtagatgg
    10501 cgtcagtatt gcgataaaag atggtgagtt cttctctatg ctggggccgt ccggctccgg
    10561 caaaaccacc tgcctgcgcc tgattgctgg cttcgaacag ctttccggcg gggctatctc
    10621 tatctttggt aaacccgcca gcaatctgcc accgtgggag cgggacgtga atactgtctt
    10681 tcaggactac gcgctatttc cgcatatgtc gattcttgac aatgtcgcct atgggctgat
    10741 ggtcaaaggc gtgaataaaa agcagcggca cgcaatggcg caagaggcgc tggagaaagt
    10801 ggcgttgggg tttgtacagc aacgtaaacc gtcacaactt tctggtggtc agcgccagcg
    10861 ggttgctatc gccagagcat tggtgaatga accgcgcgta ttgctgttgg atgaaccgct
    10921 cggcgcactg gatctcaaat tgcgtgagca gatgcagctg gaactgaaaa aactgcaaca
    10981 gtctctcggt atcactttta tcttcgttac ccacgatcag ggcgaagcgt tatcgatgtc
    11041 cgatcgtgtg gcggttttca ataatggacg cattgagcag gtcgattccc cgcgcgatct
    11101 ctatatgcgc ccgcgcacgc cgtttgttgc cggattcgtc gggacatcca acgtttttga
    11161 tggactgatg gcagagaaac tttgtggcat gacgggaagc ttcgccctgc gaccggaaca
    11221 tatccgcctc aacacccctg gtgaactgca ggccaatggc acgatccagg cggtgcaata
    11281 tcagggcgcg gcaactcgtt ttgaactgaa attgaacggc ggtgaaaaac tgcttgtgag
    11341 tcaggccaat atgacaggcg aagaactgcc tgccacgctc acgcccggac aacaggtgat
    11401 ggtttcctgg tcgcgtgatg tgatggtgcc gctggttgag gagaggtgaa tggcgatgaa
    11461 tgtattgcaa tcaccttcac gtccaggtct gggtaaggtg tccggtttct tctggcgtaa
    11521 tccggggctg gggctgtttt tactgctgct tggcccgcta atgtggtttg gcattgtcta
    11581 tttcggctcg ctgctgacac tgttatggca gggattttat acttttgacg atttcaccat
    11641 gtcggtaacg ccggaactga cgctggcgaa tatccgtgcg ctgtttaatc cggcgaatta
    11701 cgacatcatt ctccgcacgc tgactatggc tgtggcagtc actatcgcca gcgccattct
    11761 ggcttttcca atggcgtggt atatggcgcg ctataccagc gggaaaatga aagctttttt
    11821 ttatattgcg gtaatgttgc cgatgtgggc gagctacatt gttaaagcct atgcctggac
    11881 gttattgctg gcaaaagatg gcgtggctca gtggttttta caacatcttg ggctggaacc
    11941 actgctgact gcgttcctta cattacctgc ggtgggcgga aatacgctgt caacttccgg
    12001 gctggggcgc tttctggtgt ttctctatat ctggttgccg ttcatgatcc tgcccgttca
    12061 ggcggcgctt gagcgtttgc cgccgtcatt gttgcaggcg tcggctgatc tcggcgcacg
    12121 tccacgacaa acctttcgct atgtggtgct gccgctggca atcccgggta ttgccgctgg
    12181 ctctatcttt accttctcac tcacactggg cgattttatc gtcccgcagc tggttggtcc
    12241 tccaggatat tttatcggca atatggttta ttcccagcag ggggcgattg gcaatatgcc
    12301 gatggcggcg gcattcaccc tggtgccgat tgttctcatc gcactgtacc tggcgttcgt
    12361 gaaacgtctg ggagcgttcg atgcactctg aacgcgcacc gtttttcctc aaactggcgg
    12421 cctggggcgg cgttgttttc ctacattttc ccatcctgat aatcgccgcc tatgcgttta
    12481 acactgaaga tgcggcgttt agttttccac cgcagggcct gacgctgcgc tggtttagcg
    12541 tggcagcacg gcgtagtgat attcttgatg ccgtgacact gtcacttaaa gtggcggcgc
    12601 tggcgacatt aattgcgctg gtgttaggga cgctggcagc tgccgcgctg tggcgacgag
    12661 actttttcgg caaaaacgcc atttcgctgt tactgctgct gcccattgcg ctgccgggca
    12721 ttgtcactgg tctggcgcta ttaaccgctt ttaaaaccat caatctggag ccgggatttt
    12781 tcaccatcgt ggtcggtcat gcgacttttt gcgtagtggt ggtgtttaac aatgtcatcg
    12841 cccgttttcg ccgcacctcg tggagtctgg ttgaggcgtc aatggatctt ggggccaatg
    12901 gctggcaaac cttccgctac gtagtgttgc cgaatctcag ttcggcgcta ctggcaggag
    12961 gaatgctggc gtttgccttg tcgttcgatg aaatcatcgt tacgactttt acggcaggtc
    13021 atgaacgaac gttaccgttg tggttgctca atcagcttgg gcgaccgcgt gatgtaccgg
    13081 taactaacgt ggtggcactg ctggttatgt tggtaacaac cttgccgatc ctgggggcct
    13141 ggtggctaac ccgcgaaggc gacaatggtc aataaccact gatacaggaa tatgctatgc
    13201 aacataagtt actgattaac ggagaactgg ttagcggcga aggggaaaaa cagcctgtct
    13261 ataatccggc aacgggggac gttttactgg agattgccga ggcatccgca gagcaggtcg
    13321 atgctgctgt gcgcgcggca gatgcagcat ttgccgaatg ggggcaaacc acgccgaaag
    13381 tgcgtgcgga atgtctgctg aaactggctg atgttatcga agaaaatggt caggtttttg
    13441 ccgaactgga gtcccgtaat tgtggcaaac cgctgcatag tgcgttcaat gatgaaatcc
    13501 cggcgattgt cgatgttttt cgctttttcg cgggtgcggc gcgctgtctg aatggtctgg
    13561 cggcaggtga atatcttgaa ggtcatactt cgatgatccg tcgcgatccg ttgggggtcg
    13621 tggcttctat cgcaccgtgg aattatccgc tgatgatggc agcgtggaaa ctggctccgg
    13681 cgctggcggc agggaactgc gtagtgctta aaccatcaga aattaccccg ctgaccgcgt
    13741 tgaagttggc agagctggcg aaagatatct tcccggcagg cgtgattaac gtactgtttg
    13801 gcagaggcaa aacggtgggt gatccgctga ccggtcatcc caaagtgcgg atggtgtcgc
    13861 tgacgggctc tatcgccacc ggcgagcaca tcatcagcca taccgcgtcg tccattaagc
    13921 gtactcatat ggaacttggt ggcaaagcgc cagtgattgt ttttgatgat gcggatattg
    13981 aagcagtggt cgaaggtgta cgtacatttg gctattacaa tgctggacag gattgtactg
    14041 cggcttgtcg gatctacgcg caaaaaggca tttacgatac gctggtggaa aaactgggtg
    14101 ctgcggtggc aacgttaaaa tctggtgcgc cggatgacga gtctacggag cttggacctt
    14161 taagctcgct ggcgcatctc gaacgcgtca gcaaggcagt agaagaggcg aaagcgacag
    14221 ggcacatcaa agtgatcact ggcggtgaaa agcgcaaggg taatggctat tactatgcgc
    14281 cgacgctgct ggctggcgca ttacaggacg atgccatcgt gcaaaaagag gtatttggtc
    14341 cagtagtgag tgttacgccc ttcgacaacg aagaacaggt ggtgaactgg gcgaatgaca
    14401 gccagtacgg acttgcatct tcggtatgga cgaaagatgt gggcagggcg catcgcgtca
    14461 gcgcacggct gcaatatggt tgtacctggg tcaataccca tttcatgctg gtaagtgaaa
    14521 tgccgcacgg tggacagaaa ctttctggtt acggcaagga tatgtcactt tatgggctgg
    14581 aggattacac cgtcgtccgc cacgtcatgg ttaaacatta agttagatgt tagtggctgt
    14641 tataggacgg ccactatcgt tttaggttct gagtttaatg gcggttaagt attagaatga
    14701 ttacattaat aactagcgta aaaattaggc taaatgtttg atggcgattt ttcatcc
//
LOCUS       DAPGWD010000104        14480 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPGWD010000104 DAPGWD010000000
VERSION     DAPGWD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.105"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            134..490
                     /locus_tag="RHX88_003074"
     CDS             134..490
                     /locus_tag="RHX88_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV2231737.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(541..1437)
                     /gene="yhaJ"
                     /locus_tag="RHX88_003075"
     CDS             complement(541..1437)
                     /gene="yhaJ"
                     /locus_tag="RHX88_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDV2231738.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1542..2243
                     /locus_tag="RHX88_003076"
     CDS             1542..2243
                     /locus_tag="RHX88_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDV2231739.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2266..2430
                     /locus_tag="RHX88_003077"
     CDS             2266..2430
                     /locus_tag="RHX88_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231740.1"
                     /translation="MSKKLAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2642..3952)
                     /locus_tag="RHX88_003078"
     CDS             complement(2642..3952)
                     /locus_tag="RHX88_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDV2231741.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3980..5311)
                     /locus_tag="RHX88_003079"
     CDS             complement(3980..5311)
                     /locus_tag="RHX88_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV2231742.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5586..6950)
                     /gene="tdcG"
                     /locus_tag="RHX88_003080"
     CDS             complement(5586..6950)
                     /gene="tdcG"
                     /locus_tag="RHX88_003080"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV2231743.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(7022..7411)
                     /locus_tag="RHX88_003081"
     CDS             complement(7022..7411)
                     /locus_tag="RHX88_003081"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDV2231744.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7425..9719)
                     /gene="tdcE"
                     /locus_tag="RHX88_003082"
     CDS             complement(7425..9719)
                     /gene="tdcE"
                     /locus_tag="RHX88_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDV2231745.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9753..10961)
                     /gene="tdcD"
                     /locus_tag="RHX88_003083"
     CDS             complement(9753..10961)
                     /gene="tdcD"
                     /locus_tag="RHX88_003083"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDV2231746.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10987..12318)
                     /gene="tdcC"
                     /locus_tag="RHX88_003084"
     CDS             complement(10987..12318)
                     /gene="tdcC"
                     /locus_tag="RHX88_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDV2231747.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12340..13329)
                     /gene="tdcB"
                     /locus_tag="RHX88_003085"
     CDS             complement(12340..13329)
                     /gene="tdcB"
                     /locus_tag="RHX88_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDV2231748.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13428..14366)
                     /gene="tdcA"
                     /locus_tag="RHX88_003086"
     CDS             complement(13428..14366)
                     /gene="tdcA"
                     /locus_tag="RHX88_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDV2231749.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3759 a         3880 c         3371 g         3470 t
ORIGIN      
        1 tacctataaa aaacaaccat gtatttatct taaatatcaa aacctttata acatccgaaa
       61 aagaaagata cgaatagcat ttacacaata cccggaatag actttcgtca cttttaatta
      121 aagggatgtt tttatgcagt ggtatttagc cgtattaaaa aattatgttg gtttctctgg
      181 tcgcgcacgt cgtaaagagt actggatgtt tactctgatt aacgccatcg tcggcgctat
      241 tatcaatgtc attcaattga ttttaggttt agagtttcca ttcctttctc tcatttacct
      301 ggcagcaaca atcattccgg tcattgctct gtgtgttcgt cgtctgcacg atactgaccg
      361 ttcaggtgcg tgggcgcttc tctatctggt accgatcatt ggctggttag ttctgtttgt
      421 atttgcctgt ttagaaggca attctggcag caatcgttac ggaaacgatc ctaagtttgg
      481 ttcaaattaa tcttagaatt ggggcgacat ttcgcccctt tttattaata ataacaaata
      541 ttattttccg ctaaaaagtt tgggaatttc ccgcagacac caggatttcg cttcccccat
      601 actgtcacga cgccaggcca taataatatc gatctcgctg gtcgattccg ggctgacgac
      661 acgcaaccgc ccttccgcaa tatctttttc gaccatcgga tacggcatcg tcgccacgcc
      721 aagccccgcc agtaatgcct gacgtttatc ttcaatcgtg ctcaccgtta agcgcggctg
      781 tttgtccagc agctgtacgg tcaacaccgg gcgctcacga gcggtatccg ctaccgcaat
      841 tccacgatat ttcacgcgcg tcacttcaga taacggttcc ggctcctgat gaatcgggtg
      901 atcaggcgcg gcaacgtaga cgttcattaa cgtatagagc ttgcgcgagt tgatctccga
      961 cgaggaacga aaatgcatat ccggcgcgat aacaatatcc gcccgcccct gctccagtcg
     1021 ttcccacgcc cccgccagca cttcggtgat gattgccagt tgggtattgg cttttgccgc
     1081 cagtttgtcg attaacggga aaaaggcagg tgtcggtacc agcgcttcgg tcacaatggt
     1141 gagatgcgtt tcccaaccgc gcgcgagggc ttccgcatcg gtagtcagtt tatctgcggc
     1201 ttccagcaaa acgcgccctc gctccagcaa catcctcccg acattggtga atttggtgcg
     1261 atggcccgag cggtcaaaca gcaccacatc cagctcttct tccagttttt gcatggtgta
     1321 gctaagtgcg gaaggcacgc gtcccagctc atccgccgcc gccgcaaaac tgccccggcg
     1381 atcgatcgca tccataaccc gtagtgcttc cagcgttaat gccctttctt tggccatttc
     1441 gttctcattc aggaaatttg aacataccgg gcagaatatc tggctaacaa tgtagcgtcc
     1501 agccccttac cataaaagga agtaaagaga ggtcaagaat tatgattact acccgaactg
     1561 ccaggcagtg tggacaagca gactacggat ggttgcaggc ccggtatact ttttcctttg
     1621 gacactactt cgacccgaaa ttgttaggct atgcctccct gcgtgtgctt aaccaggaag
     1681 tgctggcccc aggtgccgcc tttcagccgc gaacctatcc caaagtcgat attttaaatg
     1741 tgattctgga tggagaagca gagtatcgcg acagcgaagg caatcatgtt caggccagcg
     1801 ccggtgaggc gttgctgctc tctacccagc cgggtgtcag ctacagcgaa cacaatctca
     1861 gcaaagacaa accgttaaca cgaatgcagc tttggctgga cgcctgcccg cagcgagaga
     1921 atccgctgat tcaaaaactg gcgcttaata tgggcaagca gcaattaatc gcctcgccag
     1981 agggcacagt gggtagcctg caactgcgcc agcaagtgtg gctgcaccat atcgtgctcg
     2041 acaaaggcga aagtgcgaat ttccagttgc atgggccacg cgcgtatttg caatcgattc
     2101 acgggaaatt tcatgcgctt acgcatcatg aagagaaagc ggcgctaacc tgcggtgatg
     2161 gggcgtttat tcgtgacgag gctaacatta cgctggttgc cgattcccca ctgcgcgctt
     2221 tgctgataga tttgcctgtc tagttgtttt tacagggaga tgatgatgag taaaaaattg
     2281 gccaaaaagc gccagccggt gaagcccgtg gtggcgaaag aacctgctcg caccgccaaa
     2341 aattttggct atgaagagat gttgagcgag ctggaagcta tcgtcgcgga tgctgaaacg
     2401 cgtttagccg aggatgaagc taccgcgtaa agacaggttg ccggatgcgg cgaaaacgcc
     2461 ttatccggcc tacaaaatcc aacaacatta acgaattaaa caacccgtag gccggataag
     2521 atgcgccagc atcgcatccg gcatcaggta caaaacacaa caacatcaac aagttgagca
     2581 ccccttaggc cggataagat gcgccagcat cgcatccggc acgataccca aaacctggcg
     2641 tttatctggc cttgctcgcc ataatctcga taatctgccg atccgtttgc tgcatcgaat
     2701 ggcttgctaa cgcacacagg ttggcaatcg actgctcaac atcatgcgcc acgatccctt
     2761 cattgccggt cacggcggta tcatccagcg ccattaacac cgctttccac gcagccgaag
     2821 cactggtcga aaccttcatc gcacagctgt tcgacgctcc atcgcaaatc atgccgctga
     2881 catcgccgat catactgctg atcgccatcg agatagtttc ataacgccca tccaccagcc
     2941 atgccatccc ggcggcggcc cccattgctg cggtcgttgc ggcacaaagt gcagacaaac
     3001 gcggtaactg gttatggatg taaattgcgc tcaaatgcga aagcatcagc gcacgcgcca
     3061 gccgttcatc atccgctccg aagtgttctg ctaccaccac cacaggcatt gttgcggtga
     3121 tcccctggtt acccgagcca gagttactca ttgccggaag cgtagcgccg cccatacgcg
     3181 catcggatgc cgcgctggta cgaatcacaa tggatgaaga gagatctttc gccagcaaac
     3241 cgcgctcgca ctgtttttcc agcgtcgcgc caatatgcag cccccactta ccgctcaaac
     3301 cttcctgcga taacgcacaa tttagcttcg cggaatcgag aataaagcgg atcgccgcaa
     3361 acgggacttc attgacgaac ttcaggatct cagccagcgt cgttctggaa agcaccgtca
     3421 gcggagactc ttgctcgccc tctgccacac acgcctgctg ggtaaacacc acaccattgt
     3481 gcgtctcaat atgcacaatg ttggtatgcc cgccgacgat ggtgacacac gcccacttct
     3541 caccgttcca gactttggcg cgtgagaaga ggatttcatt gcaaggttcc tggatcttaa
     3601 cggagacttt ccccgccgcc agcagtgctt tggcatcggc aattgcctgc gcagttgcgt
     3661 ctttcagcac ttccagcccg gcgttggcat ttccacctaa cgcccccagc gccgccgcaa
     3721 tcggcagccc caccattccc gtgccgggaa cggtgacgcc cagaccgttc ttcatcagat
     3781 ttggcgaaac ccaggcttct acacgttcaa ccggaccttc cagttctgcc gcagcaaccg
     3841 ccgccgccag cgccagtgaa atcggttcag tacatcccag cgccggtttt acttcctcct
     3901 gaacggcgag gatgtaacgc tgccataacg gatttaaagt cgaatcaaac atattaaaaa
     3961 ccttaaaatt tcaggtaaat caagaaaatg ccaggaacgg agaaacacaa agcaacaaac
     4021 cagtgacgat aatcaggtac agagacatcc ctttgtattt gtgcaatgcc ggtactttgt
     4081 aaaccagcca cgccgggatc aggcacccta ccatgccgaa aatcgggcta cagatagagg
     4141 tgaagctcaa caccggtgcg ttcagtacga tggcgctcca ggccagcaaa atggcgaaaa
     4201 tcatgatgcc gcgctgaaca agattttcgt taatcttctc ggcaggcatc ttgcgacgca
     4261 ggatgttcat tacgatccct tgcgttgctt cgcgaaagcc taaatagacg ccaaagaatg
     4321 cagtcatgac agcaaagata ttgagaatga cgctgaccac tttcacccac gctgcgccgt
     4381 caccgctaat aaactgtgcg gcaatcgcca gcgcggaaat attctgctca taggctttta
     4441 ccgcttcgtc atgtcccatc gccagcgtga acgacacggc gtagaaaaag acggtgacaa
     4501 acaaaatgcc aaacgcgata ttcattgccc gcaatgcttt atgccgcgcc acttcaattg
     4561 atttttcccg cgagcgataa gagatcacca tcgggcttaa cgtctggata aacagaatcg
     4621 acgtcagggt aaacggcagc gtaataatgg cgtttttcac cagcagcccc agcggcggta
     4681 gcgaaccgac gttatacaga tgccacattc ccaccatcga cacgcccagc gccgcgacca
     4741 ccagcagctt ggtcagcacc atgccggtcg aaattttgaa taacaatttc tcgccgcgtg
     4801 aagagatcgc caccagaatg caaatcagta ccagaccata aaagggactg tctgacagca
     4861 acccttccgt cacgccgaag gtatgcagat aggaagcact atcgttggtg atggcggtgg
     4921 aatagacgaa catccaaatc accagcatta cgaaatagag cgcacctaac aggatgcccc
     4981 agtttttacc taaataaccg ctaatgacac tcgggtaatc tttacattct ggtgattctg
     5041 ccagcgtatt aataaacaac cgctgaaaca gatacattgc cgggtaacca atcaccgatg
     5101 agagcaaaaa tacccacaat cccatcaaac cgacctgcac cgggagaaaa acaatccccg
     5161 cgccaatcgc catcccgata ctcataatca cccagccggt gtcagtactg tcgaatttga
     5221 tcgcttctcg ccactcgctc tcactcattc cagcacgacc cgccggggtc gaagcgtctg
     5281 caatgacgcc tttattcgat gcaatttcca taatttctcg ctcaatattt tgtagggctt
     5341 attatttttt tccgagccgc atcaaggcga tatgcggtct acgtttttgc aggcgagtta
     5401 ttagaataaa gaaatgatac gcgcgaggtc agagaaaatc ttcacaatca agactttgaa
     5461 ataacggaga tggataagaa ttttctacta aattaatcgc aagagaatat tcattctcta
     5521 tttgcggcag atcacaaaaa aaaggtgcac atttgtgcac ccaaggatga aagctgacag
     5581 caatgtcagc cgcagaccac tttaatggcc agtcctccgc gtgatgtttc gcggtattta
     5641 tcgttcatat ctttgccggt ttcatacatc gtctcgatca ctttatcgag tgaaacacgc
     5701 ggtgccgagg tgcggcgcat cgccatccgc gcggcgttta ctgctttcac ggcattaatg
     5761 gcattacgtt caatgcacgg gatttgtacc tgtccggcaa ccggatcgca ggtcagccca
     5821 aggttatgct ccatcgcgat ttccgccgca ttgcatacct gcgccggact gccgcccagt
     5881 agttcagtta accctgccgc cgccattgaa caggccacgc caatctcccc ctgacagccg
     5941 acctccgcgc cagagatgga ggcgttcatt ttatacagcg cgccaatagc ccccgctgcc
     6001 agaaaatagc gggcaattga ccgctcgttt accggacgac ggaacttatc gtaataagcc
     6061 agcactgccg gaataatgcc gcacgcaccg ttagtcggtg ccgttaccac gcgcccgcca
     6121 gctgcgtttt cttcactaac cgccagcgcg tacatgttga tccagtcgat gacattcatc
     6181 ggatcgttag agatgttatc gctggaaacc agctgacgac gcagcgctac ggcacgacgc
     6241 ggcacattga gcggaccagg cagcacgcct tcagtgttca tcccgcgttc aataccgtca
     6301 tgcatcactt gccagatacg ggcaaaccct gcgtcaattt ccgctttgct gcgcagcgcc
     6361 agctcgttgt gcatcatcag gccagatata gacaggccgt tgtaatcaca cattttcagc
     6421 agttcacctg ctgagtggaa atcgtaaggt acggacgttt cgacatcgtg cgacaggccg
     6481 aagtgttctt cttcgacaat aaacccgccg ccgacagagt aataggtttt acttaatagc
     6541 gcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg caacatttcg
     6601 ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcacc agatgccact
     6661 ggcagccgcc cgctgcgcgt tactaactct ataaatgcag ggatctcatc aatgacaaca
     6721 tcctgcggac tgtttcctgc cagtcccatg ataatggcga catccgtggc atggcctttg
     6781 cccgtcagtg acaacgaccc atacagatcg accacaatat ggctcgtcgc ggttaataag
     6841 ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc cacggtatgc
     6901 gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat atccacaccc
     6961 tcggattgcc gttcagtgaa gtggagcgga acgaccttgc gaccgtcccg ctcacgaggc
     7021 tttacgcact acgtactgcg atagcttcaa tttccagctt cacatctttc ggcaaacgcg
     7081 caacctgcac acagctccgg gtcgggtagg tcgcctgatg ctcatcgaag aactgcttat
     7141 agacttcatt gatggtggca aaatcattca gatcggtgat gaacacggtc atcttgatga
     7201 tatcgcccac gctcagcccg gcagcaacca cgatcgcttt gacgttttcg aggcttaaac
     7261 gcgcctgatc ttgcacatca gccgggatct caccggtctg cgggcaaacc ggaatttgcc
     7321 cggaggtgaa gaccatgctg cctaaatcaa cgccctgaac ataagggccg attgcgcctg
     7381 gggcacgttg cgtttcgata atctttttca tacatcctcc ggcgtcagag cgcctgggta
     7441 aaagtacgtg aaataacatc ctgttgctgt tcacgggtca gtgcgttgaa gcgcacggcg
     7501 tagccagaga cacggattgt caggttagga tatttttccg ggtgctcgat ggcatccagc
     7561 agcatttccc gattcattac gttgacgttg aggtgttgac cgccttcgac atccgcttcg
     7621 tggtggaaat acccatccag caggccgaca aggttggttt tacgtactgg atcttctttg
     7681 cccagcgccg caggaacgat tgagaaggtg tacgagatcc catctttggc gtaggtgaac
     7741 ggcagtttcg ccaccgacgt caatgaggcc acggcacctt tgcggtcacg accatgcatc
     7801 gggttagcgc ccggcgcgaa cggtgttccg gcgcgacgac cgtccggcgt attaccggtt
     7861 ttctggccgt acaccacgtt agaagtgata gtcagaatcg actgggtagg gacggcgttg
     7921 cgataggttg gcagcgcttt aattttcttc ataaagcgtt caaccaggtc gcaggcaatg
     7981 ctgtctacgc gctcgtcgtt gttgccgtac tgcggatatt caccgtcgat ttcaaagtcc
     8041 accgccaggc cgttttcgtc acggattggt ttcacgcggg catatttgat ggcagacagg
     8101 gagtccgtcg ccaccgacag gcccgcgatg ccgcatgcca tagtgcgata gacatcacga
     8161 tcgtgcagcg ccattagcga agcttcgtag ctgtacttgt cgtgcatgta gtgaatgata
     8221 ttcagcgcgc tgatgtactg caccgccagc cagtccatga agtgatcgag gctgtccatc
     8281 actttgtcgt aatccagcac gtcgtccatc agcggtgctg ttttcggccc gacctgaatc
     8341 ttcagcttct cgtccacccc gccgttaatt gcgtagagca gcgttttcgc caggttagcg
     8401 cgtgcaccaa agaactgcat ttgcttacca atcaccattg ggctgacgca gcaggcaatc
     8461 gcgtaatcgt cgctgttgaa gtcagtacgc atcagatcgt cattttcata ctgcaaggaa
     8521 gaggtgacga tcgacacctg cgcggcatat tttttgaagg caatcggtaa ttcttccgac
     8581 caaagaatgg tcaggttagg ttccggtgcc ggccccatag tgtgcagggt gtgcaaatag
     8641 cggaaggagt ttttggtcac cagcgtacga ccgtccagcc ccatcccgcc gatcacttcc
     8701 gtcgcccaga ttgggtcgcc ggagaacagc gaatcaaatt ccggtgtacg caggaagcgt
     8761 accatacgga tcttcatgat gaagtgatcg atcagttcct gtgcctgctg ctcattgagt
     8821 acgccagctt taaagtcgcg ctcaatgtag atatcgagga acgatgccgt gcggcccagc
     8881 gacatcgcgc cgccattttg cgatttcact gccgccagat aagcgaagta gagccactgc
     8941 accgcttcct gcgcattctg cgccgggcga gagatatcaa agccatattt cgccgccatt
     9001 tcctgaatct gcaacagcgc atgacgatgc tctgccagct cctcacgcag acggatggtg
     9061 gcttccagat cctcgccttt ttccagacga gactggagat cggcaaattg cagttcgcgt
     9121 tcacgtacca gataactgat gccatacagc gctacgcggc gatagtcacc gataatgcgc
     9181 ccacggccat agccatctgg taaaccggtc agcacgccag atttacggca gcgcagcata
     9241 tccggtgagt aaacatcaaa tacgccctgg ttatgggttt tacgcagatc ggtaaacaga
     9301 tattcaaatt cactgtccat ttcacggcca taggcgtgga atgaactttt aatcatatta
     9361 atgccaccga acgggtgtag cgcacgtttc aacggcgcat ccgtttgcag gccaacaatt
     9421 ttttccagcg gctggttaat atatcccgca tcatgagcgg taattgtggt ggcaatattg
     9481 gtatcgaaat caaccggcgc gtgggttgcg ttttcgatac ggataccttc cattactttt
     9541 tcccacaatt ccgtggtggc aggcgtcgct tcggcgagga aagattcatc gccttcatac
     9601 ggtgtatagt tatgttgaat aaaatcgcgg acattaattt cgtttttcca gtccgtacct
     9661 ttaaagccaa gccatgcgtc ggcgtacagc ttatcgctgg tatcaatatc taccttcatg
     9721 aaaaataatc tctctacaat acttcaacta aattatgcaa attctgcggg cgcgttaact
     9781 ttgcctaaat gaatggcatc caaagcaatc attttttctt cgttagtcgg aataacggca
     9841 caaatgacac gcgcattttc actggaaaca attcgctcac cacaggagtt agagcgatta
     9901 ttcatttctg tatcaatctc tacgcctaat acagccaaat gttccatgac cagacgacga
     9961 attaagcttg aattctctcc tattccgccg gtgaatataa ttccatccag gcgacgtaat
    10021 gaagctgcgt gtccggcaat atgacgggca attcggtgaa caaaggtttt aattgccagt
    10081 tgcgcgcgtt cgtgaccttc atgccaggct ttttccagaa cacgtaaatc cgaagaaaga
    10141 ccggaaatac ctaataatcc cgactcttta ttcactacgc gttccaggtc acccaggctc
    10201 tggttggttt ggctggcgac ccaggacatc gcaccaaagt cgacatcgcc actgcgggta
    10261 cccatcatca agccttccag cggcgtcatt cccattgaag tatcaacact ctgaccgttg
    10321 cgaaccgcgc agattgacgc gccattgcca agatgcgcca caaccaggcc ggagtcatct
    10381 tccgccagat tcagcagcga atgtgcgcgc tgggaaacat agcggtgcga cgtgccgtgg
    10441 aaaccataac ggcgtacacc taactcttca taatatttcc acggcaggcc gtataaataa
    10501 gcttccggag ccatcgtctg gtggaaactg gtatcaaata ccgccacctg agttacgccc
    10561 ggaaataatt gctgcgccga ttcaatacca cttaaattgg cgtaattatg caggggtgcc
    10621 agtggagaaa cgcgacggat attatcaatg acttcatcgg taataatggc ggactcggta
    10681 aaaatactgc cgccgtgagc gatgcggtgg ccaattaagg ccacactgtc atttaaattc
    10741 cgtttttcca gttcaaatgc aattgccttc aatgcacctt cgtagctgtg gtgagccagc
    10801 ggtgctggct ctcccccatt tacggataag aatgcatttt ccgagttaat accgtcggca
    10861 atacctgaca ttaatacttc acagtcgctg gcatcgagta cggaaaactt aatcgaagac
    10921 gaaccacagt taataaccaa aacaaccgga aattcattca tctcttttct catcctgagt
    10981 tacggattaa aacagtttgt atacgatgtt caggatggtc agcagaccaa tcacggtaac
    11041 aaacacgtta tccagacgac cacggtattt cgccagagac ggcgctttac ggatggcata
    11101 catcggcaac aggcacagca gggatgcgat aatcggtgcg cccatggctt caatcaggtc
    11161 aaggatgttc gggttggcgt aggcaacaac ccaggtggag cccatgatga agatcatgct
    11221 gatagtgttc agtttaccca gcgacacttt agttttgtcg cctttataac caaacttcag
    11281 gaccaggcca ttcagacctt ccagcgttcc cagatagtga ccgaagaaag atttgaagat
    11341 agccacgagt gcgatgatgg aagccgcata ttccagtgta atcgcgaacg ttgttttggt
    11401 accggtcatg gacgcaaagt ggttagccag ataagaaagc actggaatat tctgcgcttt
    11461 ggcttccgcc atgttggccg gagacagcgt aaacaggcag ctaaaggcaa agaacatcac
    11521 cactgcaacc atcagcatgc tggcacgaga aatgatttgg gaacatttac gttcggtgaa
    11581 gtcgcgaccg aagtctttct catactcttc acgtttagaa accacgaagg aagagacgat
    11641 tggcgagaag ttaaaggaga aaaccatgat ggaaatcccc agccagacag tgatcaggat
    11701 accgtcatga ccggttaacg acagcgaacc gaggtcaacc tggtcgataa ctgcagagtt
    11761 ccagtaaggg atcagcgaca gagaaatcag caccaggctg gcgataaacg gccataccag
    11821 gtagctcatc actttaacca tcagatcctt accaaaccag atgacgaaag ccatcagcag
    11881 caacaggaac agcgccacaa agccgcgatt cagcggtgca aatccgagct ggttttccca
    11941 gaacgtcata aaggtattgg taatagtaac gccataaatc cacagcagtg ggcaaatcgc
    12001 gaagaagtac aggaacgtga taaccacgcc gccagtttta ccaaaatgct cttccaccgt
    12061 ttccgtaatg ttgccggaag ggttagagcc agaaagacac agacgcgcca gcgcccggtg
    12121 gcaataaaat gcgatggggt atgccaatac caacatcaga agaatcggga tcagtccgcc
    12181 aaaacctgcg cggataggga agaacagcac cccggcgccg attgccgtac caaacaagcc
    12241 taacgtccat gtggtatctg atttacgcca ggacgattgt tttgtctggc tggatacaat
    12301 gctatctgaa gtactcatat cctatcctca acgaattaat taagcgtcaa cgaaaccggt
    12361 gatttgagag acgcgagaaa gatcgatatt gccgccggaa ataatactga cggtttttct
    12421 gttttgaata tattggtcta atttaccgct taataatgca gcacatgcca gagcgcctgc
    12481 gccttcggtg acgactttat tgcgctgaat taaggcaatc atactgtttc tgatttcgtc
    12541 ttcgctgacc agcacgatgt catcgactaa ttcacgaacg atttcgtaag ttaaattacc
    12601 cgggcgggag acatcacaac catccgccag ggtgccggta gttcggtgcg tggttatttc
    12661 tccggagtgg aaagaagccg ccatgccgtg aacgttttct gactgtacgc caataacacg
    12721 aatggtcggg ttaatagatt taattgccac cgcaatacca gcaattaaac caccaccacc
    12781 aattggcaca atcacgttat cgacatcata gagatcttcc ataatttcca gaccaatcgt
    12841 tccctggcca gcaatcactt tcggatcatc gtaaggtggg ataaaaatac gaccttccat
    12901 ttcgacaatt tcgctcactt tagcgatagt gtcgttgaaa ttatcaccat gcagaacgac
    12961 ttctgcggag tagtcgcacg ttgccgctac tttggatttt ggcgcacctt ttggcatcac
    13021 cactttaccg tcgataccca gcatcgcgca ggagagcgaa accccttgcg catggttgcc
    13081 cgcagaacag gccactacac ctttacgttt ttccgcatca gtcagtgaac ttaatttatt
    13141 aaatgcgcca cgaattttaa atgaacccgt acgctgcata ttttcaaact tcaggaatat
    13201 ttcacctttg caacgttcac taaaatagtt tgagcgaggc atgcctgttt tataaattcg
    13261 cccagccagt cgttgtttcg cttcaataat gtcatcaata gcaaccggca gatcgtatgt
    13321 aatatgcatt ataaaacctc ttcgcctgaa ttaaatatgt aggtaaccgt aaccgacacc
    13381 tgcaaaacag acaggtggat tatttatgtt taaataaaaa caaataacta accaacttct
    13441 attaattgcc ttcgtctaca cccattataa gatgaatact ctttggctaa ttccaccaaa
    13501 accgatgctg cttttttaat acgataattt ttcgaccata ccgcggcata ttgtgccaca
    13561 ggtaatgttt cttcaaccgg aatagtaata aattgatttg aaccaaaagg tgacgtcata
    13621 tcacaaggaa ttacagttaa gaaatcagca ttgagaacaa gattataaat tgtcacgact
    13681 gagtcggttt taacgatgtt ttcaatactg atgccatttc tttgtaacgt agtaagaagc
    13741 tcgctgtagt accccatatt ggtttgtggc aacacccact gttcgttctt caacgactcc
    13801 agcgtggtgg tgccggtgca tgttcgggac ttactggcta ccagcacaaa ctcggactcg
    13861 aacagcggct caacatgtaa atcctgaagc ttcatttctg cacttaacgt accaatcgca
    13921 aaatccagtc gaccgtcgcg gattgccggt aagaacgaag acagttgcgc ttcatacata
    13981 gaaacctgcg ctttcgggaa cacctctttg aacttgttga tcatccctga cataaaagta
    14041 aaaccaatca atgaaggaaa accaaatgag acttccacca ccgcctcaga agacataccg
    14101 cttatctcat taaccatatt tttcatttca cgggtaatgg attcggaacg ggagagtaac
    14161 aattgaccgg caggtgttaa tgttacaccg gtatttttcc gcaccactaa ttccacacca
    14221 aaataatctt caatatcgtt aatgatttta ctgacggctg gttgagttaa ccctaattct
    14281 tttgcagccg agccgataga gccacttcta atgacttcct gaaagactac caggtgctgc
    14341 gttttcggaa gaagaatagt gctcataacg acctacgtta attacctcat tgacggcatg
    14401 aagtgtatca aaatgaaatg aacaggatat gtgcgaccac tcacaaatta actttcaata
    14461 ctttccaggg tatcgttatt
//
LOCUS       DAPGWD010000105        14435 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPGWD010000105 DAPGWD010000000
VERSION     DAPGWD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.106"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            87..653
                     /locus_tag="RHX88_003087"
     CDS             87..653
                     /locus_tag="RHX88_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2231750.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            704..3163
                     /locus_tag="RHX88_003088"
     CDS             704..3163
                     /locus_tag="RHX88_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2231751.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3178..3909
                     /locus_tag="RHX88_003089"
     CDS             3178..3909
                     /locus_tag="RHX88_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2231752.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3906..4967
                     /locus_tag="RHX88_003090"
     CDS             3906..4967
                     /locus_tag="RHX88_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2231753.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5119..6165
                     /locus_tag="RHX88_003091"
     CDS             5119..6165
                     /locus_tag="RHX88_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDV2231754.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6162..6953)
                     /gene="nei"
                     /locus_tag="RHX88_003092"
     CDS             complement(6162..6953)
                     /gene="nei"
                     /locus_tag="RHX88_003092"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDV2231755.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6989..7723)
                     /gene="pxpA"
                     /locus_tag="RHX88_003093"
     CDS             complement(6989..7723)
                     /gene="pxpA"
                     /locus_tag="RHX88_003093"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDV2231756.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7713..8645)
                     /gene="pxpC"
                     /locus_tag="RHX88_003094"
     CDS             complement(7713..8645)
                     /gene="pxpC"
                     /locus_tag="RHX88_003094"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDV2231757.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8639..9295)
                     /gene="pxpB"
                     /locus_tag="RHX88_003095"
     CDS             complement(8639..9295)
                     /gene="pxpB"
                     /locus_tag="RHX88_003095"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDV2231758.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9318..10061)
                     /gene="ybgI"
                     /locus_tag="RHX88_003096"
     CDS             complement(9318..10061)
                     /gene="ybgI"
                     /locus_tag="RHX88_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDV2231759.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10332..11813
                     /gene="dtpD"
                     /locus_tag="RHX88_003097"
     CDS             10332..11813
                     /gene="dtpD"
                     /locus_tag="RHX88_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDV2231760.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11855..13273)
                     /gene="phrB"
                     /locus_tag="RHX88_003098"
     CDS             complement(11855..13273)
                     /gene="phrB"
                     /locus_tag="RHX88_003098"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDV2231761.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13270..13779)
                     /locus_tag="RHX88_003099"
     CDS             complement(13270..13779)
                     /locus_tag="RHX88_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDV2231762.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13930..14435)
                     /locus_tag="RHX88_003100"
                     /pseudo
     CDS             complement(13930..14435)
                     /locus_tag="RHX88_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3479 a         3861 c         3511 g         3584 t
ORIGIN      
        1 tttaagaaaa actaaaaaca ttcgacaatc aaataagata ttcttgcacc gatatacttt
       61 tttcttttct gttaaaggac tccgttatgt ttaaaggaca aaaaacattg gccgcactgg
      121 ccgtatctct gctgttcaca gcacctgttt atgcggctga tgaaggatcc ggtgaaattc
      181 actttaaagg tgaagttatt gaagcaccgt gtgaaataca tcaggatgat attgataaag
      241 aggttgaact cggtcaggtg accaccagcc acattaatca gtcacatcac agcgatgccg
      301 ttgctgtcga cctgcgctta gtcaactgtg atctggaaaa ctccagcaac ggttccggtg
      361 gcaagatttc taaagttgca gtcacctttg atagctcagc gaaaaccacc ggcgcagatc
      421 cgattctcaa caacaccagc acgggtgaag ccaccggcgt cggcgtacgt ttaatgaata
      481 aagatcaaag caatatcgtt ttaggcaccg ctactccaga tatcgacctg gctccgacct
      541 ccagcgaaca aacgctgaat ttctttgcct ggatggaaca aattgatcag gctacacctg
      601 taacgccagg cgcagttacg gcgaatgcaa cctacgtgct ggattataaa taagtattac
      661 tttggcggcg ctatcgccgc ctttcataaa atatttatca ggtatggaca ccgtgaatat
      721 ttatcgactc tcttttgtat cctgcctggt cgtggcgatg ccttgcgcat tggcggtcga
      781 attcaacctt aatgttctcg ataaatcaat gcgcgaccgc attgatattt cattattaaa
      841 ggaaaaagga gtcattgctc ccggtgagta ttttgttagc gttgcggtaa ataataacca
      901 aatcagtaac gggcaaaaga ttaactggca caaaaatgac gataaaacca ttccgtgcat
      961 caatgattta ctggtcgata aatttggctt aaaacctgaa gtccgtcagt cgttaccatt
     1021 gataaatcag tgcgtcgatt ttagctcccg acctgaaatg ctcttcaatt tcgatcaagc
     1081 caatcagcaa ctaaatatca ccattccgca agcctggctg gcgtggcact cagaaaactg
     1141 gaccccaccc tccacatgga aagaaggtgt cgccggtgtc ctgatggatt acaacttgtt
     1201 tgccagcagc taccgcccac aggatggcag cagcagcact aatcttaacg cctatggaac
     1261 agccggaatc aacacggggg catggcgctt acgcagtgat tatcagttga atcagactga
     1321 tagcgatgat aaccatgaac agtcaggcga aatatcacgt acctatcttt ttcgcccgtt
     1381 accgcaatta ggttcaaaat taaccctcgg cgaaacggat tttagttcca atattttcga
     1441 cggtttttct tataccggcg cggcactggc aagtgacgat cgaatgttgc catgggaact
     1501 acgcggctac gccccacaaa ttagcggtat tgcacagacc aatgccacgg tgacgatcag
     1561 tcaatcaggc cgcgtcattt accagaaaaa agtcccacca ggcccattta tcattgacga
     1621 ccttaatcag tctgttcagg gcacactgga tgtcaaagtg acggaagaag atggtcgggt
     1681 gaacaatttc caggtttcgg cagcatcgac gcccttcctg actcgtcagg gacaggttcg
     1741 ctataaactg gccgcgggtc agccacggcc ctccatgtca catcaaactg aaaatgaaac
     1801 cttttttagc aatgaagttt cctgggggat gctgtcaaac acctcgctgt acggcggcct
     1861 gctgctttct ggtgatgact accattctgc cgcaataggt atcgggcaaa atatgctgtg
     1921 gcttggcgcg ctgtcgtttg atgtcacgtg ggccagtagt cactttgata ctcagcagga
     1981 cgagcggggc ttaagctacc gttttaatta cagcaaacaa gtggatgcca ccaacagcac
     2041 gatttccctc gccgcttatc gtttctccga tcgtcatttt cacagctacg ccaactatct
     2101 ggatcacaaa tacaacgaca gcgatgcgca ggacgaaaaa cagacgatca gcttatctgt
     2161 gggccaaccg attaccccac taaacctcaa tctttacgcc aacctgctac atcaaacctg
     2221 gtggaatgca gacgcctcca cgaccgccaa catcacagcc ggttttaatg ttgatattgg
     2281 tgactggaga gatatctcta tttcgacgtc attcaataca acccattacg aagataaaga
     2341 tcgcgacaac cagatttacc tgtcgatttc gctccccttc ggtaacggtg gtcgggttgg
     2401 ctatgacatg caaaacagta gccacagcac cacacaccgc atgtcgtgga acgatacgct
     2461 ggatgaacgt aatagctggg gcatgtctgc cggactgcaa tccgaccgtc ctgacaatgg
     2521 agcccaggtg agcggtaact atcagcacct gagttcagcg ggtgagtggg atatttctgg
     2581 tacctatgcc gccaatgatt acagttccgt cagcagcagc tggagcggtt ctttcaccgc
     2641 aacccaatat ggtgcagcgt ttcatcgccg cagctccacc aatgaaccac gcctgatggt
     2701 cagcaccgat ggcgtggcag atattccggt tcagggcaat ctcgactaca ccaaccattt
     2761 tggcattgcg gtggtgccgt tgatttccag ttaccagcct tccaccgtgg cggtgaacat
     2821 gaatgactta cccgacggcg taacagttgc agaaaacgtt atcaaagaaa cgtggattga
     2881 aggcgcgata ggttacaaat cactggcttc ccgttccggt aaagacgtta acgtcatcat
     2941 tcgcaacgcc agcggtcagt ttcctcccct cggagcggat atccgccagg atgacagcgg
     3001 cattagcgtg gggatggttg gcgaggaagg acatgcctgg ttaagcggag tcgctgaaaa
     3061 tcaaaagttt accgtggtct ggggtgatag ccagcattgc tcgctccatc ttcctgaaca
     3121 tatggaagac accgcaaatc gcctgatttt accttgtcat taattaagga aatgaaaatg
     3181 acatttatga aaggactacc ccttttgttg ttggttgcca gtctgtgcag ccacgctgca
     3241 ctacaacccg atcgcactcg tattgtgttt aacgctaatg ataaagctac cagtctgcgg
     3301 gtggataatc gtagcgataa actcccctac ctcgcttatt cctggctcga aaatgaaaaa
     3361 ggggagaaaa gcgatgatct tctggtcgcc ctgccgccga tacaacgtct ggaaccgaaa
     3421 gcaacaacgc aggtacggat agtaaagcaa gcctcgacca caaaattacc gggcgatcgc
     3481 gaaacgctat ttttctacaa tatgcgtgaa attcctcccg ccccggaaaa aaacagcgac
     3541 catgccgttc tgcaagtcgc cattcagagt cgtatcaagg tgttctggcg accggctgca
     3601 ttacgcaaga aagcaggaga aaaggtcgaa ctgcagttac aggtcagcca gcagggcaac
     3661 caactgacac tgaaaaatcc taccgcgtat tatctgacca ttgcctacct gggacgaaat
     3721 gaaaaaggcg ttctccccgg cttcaaaacc gtgatggttg cgccttttag tacggtcaac
     3781 acaaacaccg gaaattatag cggcagccag ttttatctcg gttatatgga tgattacggc
     3841 gcgttacgca tgacgacgct caactgcagc ggacaatgcc atttacaagc cgtggaggcg
     3901 aagaaatgag tgctggcaag ggagtgttgc tcgtcatctg tctgttattt ctgccgttga
     3961 agtctgcgct tgccctgaac tgctactttg gttcatccgg tggctcggta gaaaaatcag
     4021 aagcgattca accttttgct gtaccaggca atgccaaact cggcgataag atttgggaat
     4081 ctgacgatat taaaattccc gtctattgtg acaacaatac caacggcaat tttgaaagtg
     4141 agcatgttta tgcctgggta aacccctacc cgggggtgca ggatcgctat tatcaactcg
     4201 gcgtaactta caatggcgtt gattatgacg ccaaccaggg aaaaagccgc atcgacacta
     4261 accagtgtat tgacagtaag aacattgaca tttatacccc tgagcagatt atcgcgatgg
     4321 gatggcagaa caaaatttgc tcgggtgatc ccgctaatat tcacatgtca cgtacctttc
     4381 tcgctcgcat gcggttatac gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta
     4441 cgcttagcga ctatatcgtc gtgcaatttg acggtgccgg tagcgttaac gaagatccta
     4501 ctgcccaaaa cctgaaatat catattaccg gtctggaaaa cattcgcgtg ctggattgca
     4561 gcgtcaattt ttccatttca ccggaaacac aggtgattga ttttggtaaa tttaatttgc
     4621 tggatattcg tcgccacaca atgtcgaaaa cattcagtat taagacgaca aaatcacaaa
     4681 atgaccaatg caccgacgga tttaaagtca gttcctcctt ttataccgaa gaaacgttgg
     4741 ttgaagaaga taaagcattg ttgattggta atggtctgaa gctacgtttg ctggatgaaa
     4801 acgcctcgcc ttataccttc aataaatatg ctgaatacgc cgatttcacc agcgacatgt
     4861 tggtctacga aaaaacctat acagctgaac tttcgtccat tgcaggtacc cccatcgagg
     4921 ctgggccctt cgatacagtg gtgcttttta aaattaacta taactgattt gccatgatgc
     4981 cctttgcagc cggaaagggc atctctttca gaaaattgct gattttcccc ttttccttga
     5041 ttgctgttat tttttcctgg cccggctttc tttatgctgt atcgatacac caaagagaaa
     5101 ataatgaggg agcgaaggat gccagttttg cagtggggaa tgttatgtgt gctatcactt
     5161 ctcctttcta ttggtttcct cgcggtacat ctcccggcgg cgctattact tgggccgatg
     5221 atcgccggga tcatcttcag tatgcgcgga attactctgc aacttccccg ctccgctttt
     5281 ctcgccgcgc aggccattct tggctgcatg attgcgcaaa acctcactgg ttcgattctc
     5341 accacgctgg ccgccaactg gccgatcgtg cttgctattt tactggtgac gctgctttcc
     5401 agcgccatcg tgggttggtt attggtgcgc tatagctcac tgcctggaaa taccggtgcc
     5461 tggggttctt ctccaggcgg cgcagcggca atggtcgcta tggcgcaaga ttacggcgca
     5521 gatattcgcc tggtggcgtt tatgcaatat ctgcgagtgc tgttcgtcgc gggggccgct
     5581 gttctggtca cccgcatgat gctgggtgat aacgctgaag cggttaacca gcaaatcgtc
     5641 tggttcccgc cagtgagcat taatctcctg cttacgattt tgctcgcagt cgttgccggt
     5701 acggcgggat gcatgctgcg tcttccttcc ggcacgatgc tcatcccgat gctggcgggc
     5761 gcagtgctcc agtctggtca gctcatcacc atcgaactac cggaatggct gctggcgatg
     5821 gcgtatatgg caattggctg gcggattggt cttggtttcg ataaacaaat cttactgcgg
     5881 gcactacgcc cactaccgca aatcctgctg tcgatttttg ctctgctggc tatttgtgca
     5941 ggtatggcgt ggggactgac ccggtttatg catattgatt ttatgactgc ctacctcgcc
     6001 accagtcccg gcgggcttga taccgtggcg gtcatcgccg cagggagcaa tgccgatatg
     6061 gcgcttatta tggcgatgca aaccctgcgc ctgttcagta ttttgctgac ggggccagcc
     6121 attgcgcggt ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg gcagcctgga
     6181 caccagtaaa acgggcgcga ggacaacgtg gttttctcaa tgatgccgcc acagcgttcg
     6241 cacagttcgc catcacgatg aaaaacctta aagcgaaaca gcgccccatg atgcttattc
     6301 tcatccacct gcccccgcgt agcgtaagaa aaccgaggaa tctccagtaa cgcgtgtgcg
     6361 agtgcatcca gttgcgccgc attgagatct ttcgctttat gatttccagt caacccaacc
     6421 tgccagagga tctccacccg caaataattg ccaagcccag ccagaaacgc ctgatcgagc
     6481 agtaatcctg caaactgacg gttacgaaag cgcggtgaca ataaccgttc ctttaccacc
     6541 tccggcgtca gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa cggatgcgtg
     6601 gtcagttgtt ccggcctcaa catctcaata tccgaggcgc tataaagcag aatggttttg
     6661 tcagccgttt gcagttttac ccgcaatact cgcgtggtct gcggctcttc gccggtatca
     6721 accacgcgcc agacgccgta aagctgatta tggctgtaga gcgttaagtc gttggaaaaa
     6781 tgagttaaca acgccttacc acgcgtttcc acatgggtaa cgtgttgacc gataagttgt
     6841 gattgataag tttttaactg cgggaaggca aaccagacat cagttagtgg tttgcctttg
     6901 atcgccgcct ccaggttatc cgctgcacgg cggatctccg ggccttcagg catggtgata
     6961 tccttttttc attctttatt aatcggggtt atgctgcgac aacaatctcc ttttcggcaa
     7021 atgtagagcg tagtcggcgg gcgaacgcca gtgcgtgctc gccgtcgcca tgcaggcaga
     7081 ctgtttgcgc cgtgaccgtt gcccattcgc cggtgatact tttgactctg ccgtgttgca
     7141 ccatctccag cgtttgcgcc agcgcctgtt gttcgttttc aatcaacgcg cctggctgac
     7201 ttcgcggcac cagcgagccg tcagcctgat aaccgcgatc ggcaaacact tcctcgcgcg
     7261 ttgtcaggcc atattgcttg cctgcacgaa tcagctcgct tcctgccagc ccaacgagaa
     7321 ccaatgctgg atcgcaagcg tataccgctc tggcgatggc gtctgccagt tgtgcttctt
     7381 tcgccgcctg gttgtacaac atgccgtgcg gtttgacatg acgcattacg ccgccttgcg
     7441 cacgggcaat cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa acggtttctg
     7501 gcggcagctg catggcgctg cgaccaaaat tttccctgtc gggaaaactc gggtgagcgc
     7561 caatcgcgac accatttttt attgcttcac gcacgcaagc ctgcatggtt tgggcatcgc
     7621 ctgcatgaaa tccacaagca atattggcag aggaaaccag cgttaatagc tctgcgtcgc
     7681 tggcgcagcc ttcgcccaga tcggcgttca ggtcaatttt cattgtgtag ccgccacgct
     7741 aattgttcga aataacgttg ttgatcttgc cgcgctttca gtgcctcttc cagtgaacac
     7801 tggacaaaat gaatcggctg accgagcgga atttgcgcca gatggtacat atcagcctca
     7861 atgatacagg caatacgcgg gtaaccaccg gtggtctgtg cgtcgttcat caacacaata
     7921 ggctgcccgt tatgcggcac ctgcaccaca cccggtaaca aaccgtgaga taacagttcg
     7981 cgatcggtgg tgcgttttaa aatttgcccc tgtaagcgat agcccatgcg gttactttgc
     8041 gggctaagct gccagggcga acgccagaat gcatcctgcg aggcgcgatc gaactcatga
     8101 tattccggcc ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt aacgccctgt
     8161 gcttccataa aatcacgctt cgatttgcca atcgggagtc ggtcaccatc cttcagtaaa
     8221 cggccttcca gcccgccaat ccccacgttg agatcggtgc tgcatgaccc cattaccggc
     8281 ggaacatcaa taccacccgc gaccgccaga taactgcgca tgccgtgctg cgggcgttta
     8341 agcgttaaac gctggcctgc tttcatcggc aagcgccagc cggtccagac ggcgttatca
     8401 tccagccgcg cttcgcaacc ggcacccgtc agagcaaacc acccatcagt ttcgaactca
     8461 accgttaact gaccgagcgt gatctccagc gcgggggcat tggcgtcatt acccaccagc
     8521 aggttagcaa tgcgtaacgc aggcatatcc agtgcgccgc agtggctgat acccgactgg
     8581 cgaaaaccgt gacgaccgcc atcctgcaca gtggtataca tgcccgcacg aataatcttc
     8641 agcatactcc ctccttctgc ggtacaaagc gcacgctgtc tcccggacgt aataagatgg
     8701 gttcgtcacg cgccggatca aacaggctga gtgaggtatg accaatcaac tgccagccac
     8761 ccggcgttgc cagcggataa acaccagtct gcggcccgcc gatcccgaca gaacctgccg
     8821 gaacgagcaa gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc aaactcccga
     8881 gatacgggaa gcccggttga aaacctaaaa accagaccac gtattccacg gaggagtgca
     8941 attcaacaac ctgtttttcg ctcaacccgc aatgcgccgc gaccaccgcc aaatccggtc
     9001 cgcctgcacc accgtaaacc accggaattt caataaagcg agactcaggt tccagggcct
     9061 cgctctcctc ccaccagcgt tgcaaacgct ctatggcatc cagcgccagc gactcaggat
     9121 tacgcaaaat caccgtgata ttgttcatgc cgggaatggc ttcaaccaca ttcggcatat
     9181 ccaccagacg ctgcgccagt cgccagatcc gtttctggct agccagcgtc accggcggtt
     9241 ccagttccag cactaccgcc gtttcaccta tcagataaca acgcgctcgt tgcactttcg
     9301 atccctctga ttattcgtta tgcaggatta ggaatatcaa taaaggtcac atcaagatcg
     9361 gtattttcat tcagccactc gctcaatgcg cgaataccac cacgttcagt ggcatggtga
     9421 cctgcagcat aaaaatgcaa cccctgctcg cgggctgaat gaatggtctg ttcagaaact
     9481 tcgccagtaa taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa actttgcccg
     9541 ccgcccgtgc accaggcgac gcgctggacc acctcaggtc cggtatcgcc acaccataac
     9601 ggcttacgtc ccagacgcgc ttcaatccag gaggctagtt ccagtcccgg cacaggcatg
     9661 gtcagctctc cccacggcac caacggctca atttcgccca tgaccgtgat ccccagtaac
     9721 gccgccagct gtgcgttatt gcccagctca ggatgtgcgt caagcggcaa atgccagcca
     9781 tacaggttga tatcattcgc cagcaacgtt tttaaacggt tacgcttcat tccgcgaatg
     9841 accggagact ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc cgcgcccaga
     9901 cgcactgcct catcgagcaa agcctggctg gcggtgacac cggtaacaat tttttgcacc
     9961 gtctctttgc cttccacctg caaaccattc ggcgcgtaat cactaatcgc cgcgctgttc
    10021 agtttttcgt taatcagttg ttccagttcg gtgtttttca tcttcgcgcc ttccgtattc
    10081 tgatagctac accgttaaca tagcgttacc tcgcctgttc gtcgatagcc aatcttttga
    10141 catattaaga aacaaccccg acgccaaaag acgcaaattg tttatatcat cacgcttcag
    10201 ctaatgtata tttatgcaat cagacagcat attgcataaa taatcagatc tgtatcaaag
    10261 ttttaacttt aaggaaacac aagcacgccg cgcaactatt acgtgacgtt gccgtcaatt
    10321 gtggaattat tatgaataaa cacgcatcac agccgcgcgc tatttactat gtcgttgcgc
    10381 tgcaaatctg ggaatatttt agcttttacg gcatgcgtgc cctgctgatt ctctatctca
    10441 ccaatcaact aaaatacaac gatactcacg cctacgagtt atttagcgcc tactgttcgc
    10501 tggtgtatgt cacgccaatc ctcggtggct ttttggcgga taaagttctc ggcaatcgca
    10561 tggcggtgat gctgggggcg ttattgatgg cgatcggtca tgtggtgttg ggtgccagtg
    10621 agatccatcc gtcattcctc tatctgtccc tggcgataat cgtttgtggc tatggtctgt
    10681 ttaaatccaa tgtcagttgc ctgctcggcg agctgtatga gccaaccgat ccgcgtcgtg
    10741 atggcggttt ctcgctgatg tatgcggcgg gtaacgtggg gtctattatc gcacctatcg
    10801 cctgtggtta cgcccaggaa gagtacagct gggcgatggg ctttggcctg gcggcggttg
    10861 gcatgatcgc gggtctggtc attttcttat gtggcaatcg tcatttcact catacccgcg
    10921 gcgttaacaa aaaagtactg cgtgcgacaa actttctcct gccgaactgg ggatggctgc
    10981 tggttctgct ggtggcaacg cctgcactga ttaccgtgct gttctggaaa gagtggtcgg
    11041 tatacgcctt aattgtcgcg actattattg gcctgggtgt actggcaaaa atttatcgca
    11101 aagcagaaaa ccagaaacag cggaaggagc tgggactgat tgtgacgctc accttcttca
    11161 gtatgttgtt ctgggccttc gcacaacagg gcggtagctc gattagcctt tatatcgacc
    11221 gcttcgttaa ccgcgatatg tttggttata ccgttccgac cgcgatgttc cagtcgatta
    11281 atgccttcgc agttatgctg tgcggtgtgt tcctggcgtg ggtggttaaa gaaagcgtcg
    11341 caggtaatcg taccgtgcgc atctggggaa aatttgctct tggccttggc ctgatgagcg
    11401 ccggattctg cattctgacc ttaagcgccc gctggtccgc aatgtatggt cactcttctc
    11461 tgccactgat ggtgttaggc ctggcggtga tgggctttgc ggaactgttt atcgacccgg
    11521 tagctatgtc gcagattacg cgcattgaaa tccccggcgt gaccggcgta ttaaccggca
    11581 tttatatgct gctttccggc gcgattgcga actatctggc gggcgtgatt gccgatcaga
    11641 catcgcaggc ttcgtttgat gcttccgggg cgatcaacta ctccatcaat gcatatattg
    11701 aagtatttga tcaaattacc tggggcgcac tggcgtgtgt aggactggta ctgatgattt
    11761 ggctgtatca ggcgctgaaa ttcagaaacc gcgcgctggc gctggaatct taataggcca
    11821 gcaccatacc tgataaaacg ctcgctgcgt tttatcaggc tcctttcctc gcttcttcgt
    11881 acgctgccag cgttcgcagc cttgcctctt tgtgatcaac tatcggttgc ggataatcca
    11941 gcatcacacc tgctttctgc gcccacttcc acggctcatg caccgctttc cctggtacat
    12001 cgcgcagctc cggtaaccat cggcggataa actcaccttc gcggtcaaat ttctcgccct
    12061 gggttgtcgg gttgaaaata cgaaaatacg gcgctgcatc agtacccgta gacgccgccc
    12121 actgccagcc accgttattg gctgccaaat caccatcaat cagctgcgac atgaaatatc
    12181 gctcgccttc gcgccagtcg atcaacaaat ctttcaccag aaaactggct gaaatcatcc
    12241 gtagccggtt atgcatccag ccagtgctgt taagctgacg catggcggca tcgacaatcg
    12301 ggtatcccgt tttgccttcc tgccaggcct gtaaatgtgc gggattgctc tgccactgca
    12361 cacgatccgt ccaggcaata aacggacaat gtttacacaa cgaggggtaa tacgtcatca
    12421 gatgacggta gaactcgcgc cagatcagct cactaagcca gacactaccg gccccaccgt
    12481 ccagcgcctg cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc gataacccgc
    12541 ccgttgccag gctggcggac aaacgactgg tgccttccac tgccggaaaa tctcgttgtt
    12601 gctcatattc tccggcaccg ttctggcaaa actggcgtaa ttgcgcaatc gccgcttttt
    12661 cttccaccgg gaaatgcgca gtatcgaaag actgacgagg ataattcagc gtaatggatg
    12721 gcgagggctc tatcgatccg ctactacgaa cttttggcgc agcgacgcac tccggcatcc
    12781 cttcccgcag ccgtttcagc caggcattct taaaaggcgt aaagactttg tacatctcgt
    12841 gattaccggt catcaccgcg ccaggcggca ggatcacgct gtcatcaaat ccttcacaca
    12901 ccacgttacg cagcgctctt tcaacttcca catcccgcgc ccgctcattc acttcatact
    12961 gatagttata aaacaggtgg gtaacgctgt tttccgcgca cacctgttta actatttcga
    13021 cactggcgac aaagtcatcc acttcacgga acaataaagg aatacctttt tccgcaagcg
    13081 ctatttgtag cccattcagt tgagcattga taagttcagc ctgacgcggc gacatgttat
    13141 gcgtcgccca ctggcgtggt gtagcgatat acaacgccag cacgcgtgca gacgaattgc
    13201 ggcaggcggc agccagtgcg agattatcgt gcagacgtaa atcctggcga aaccagacca
    13261 gatgggtagt cataaaactc ctgaatggcg caagttaatc acacgcggcc aaagttcgaa
    13321 ataacgctgc cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt tgatacgcat
    13381 cagcggcgca agaagtggtt gctcgccacg gcgataactg tcgataagcg cagccagctg
    13441 ctggcgttct gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca tcaaaacatt
    13501 ggtgtgatcg cgcgggttgg cggggtgaga aagcaaaaca actacgcgtt ggcggtactg
    13561 gttgtaatag tcatccagat tttgccactg gtgaatatca gccacgaacg ggccaagttt
    13621 gcggtactcc ggctgagaat gcgccagaaa gacgagttta tagcggctgt gataatcgag
    13681 aagcgccccg cgcgttaagc catgttcttt cagtcgatgt aactcatgaa gggcgaagat
    13741 acggattagg gtgctgtcat caaatcgttg ctgattcatt actgctcctg aacggtttac
    13801 aggagtaagt gtagacgttg agaaatgata tttgtgaagt taatcctgtt gtagcaggac
    13861 aaatttacga aagcctgctc cccgcaagga ctgacgtcag gtagtttcta accatagctg
    13921 ccctttctat tcaaacgtga aatcaataag ctcatcaggg acatcgcaaa caatatgaag
    13981 acgggtttct tctctgccgt gactcttttc actaattgcg taactgtcat gcttgggatt
    14041 atttaattct ttcagcggaa atttttcctc gaaggcttta tttagccgcc cctggtttcc
    14101 ttttacagcg aataaataat cacctccctg tttttgtatc ttctctgcaa tatctttctg
    14161 gcaacccatc gcatcggttg tgatgatttt tcctttaata tccagcatgt taagaagttc
    14221 tgggatagct gtgatctcat tggatttctt atccgtcttg atctgcccga tgaccagact
    14281 gtgcattgtt gagaacgcac taatgacatg aatcgctccc ctgcggcgac tcttgtcata
    14341 agagtgccgg agcgtttttc catcaattgc aatgacgtct ttatcatttg atgaatggca
    14401 atcacgcatc cagttaataa agcactcgtg aaatt
//
LOCUS       DAPGWD010000106        14202 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPGWD010000106 DAPGWD010000000
VERSION     DAPGWD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.107"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            162..1220
                     /gene="citC"
                     /locus_tag="RHX88_003101"
     CDS             162..1220
                     /gene="citC"
                     /locus_tag="RHX88_003101"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDV2231763.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCNWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1235..1531
                     /gene="citD"
                     /locus_tag="RHX88_003102"
     CDS             1235..1531
                     /gene="citD"
                     /locus_tag="RHX88_003102"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDV2231764.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1528..2436
                     /gene="citE"
                     /locus_tag="RHX88_003103"
     CDS             1528..2436
                     /gene="citE"
                     /locus_tag="RHX88_003103"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDV2231765.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2447..3979
                     /gene="citF"
                     /locus_tag="RHX88_003104"
     CDS             2447..3979
                     /gene="citF"
                     /locus_tag="RHX88_003104"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDV2231766.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3983..4534
                     /gene="citX"
                     /locus_tag="RHX88_003105"
     CDS             3983..4534
                     /gene="citX"
                     /locus_tag="RHX88_003105"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDV2231767.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4509..5387
                     /gene="citG"
                     /locus_tag="RHX88_003106"
     CDS             4509..5387
                     /gene="citG"
                     /locus_tag="RHX88_003106"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDV2231768.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5438..6901
                     /gene="citT"
                     /locus_tag="RHX88_003107"
     CDS             5438..6901
                     /gene="citT"
                     /locus_tag="RHX88_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDV2231769.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7015..7821
                     /gene="rna"
                     /locus_tag="RHX88_003108"
     CDS             7015..7821
                     /gene="rna"
                     /locus_tag="RHX88_003108"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDV2231770.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8051..8461
                     /gene="rnk"
                     /locus_tag="RHX88_003109"
     CDS             8051..8461
                     /gene="rnk"
                     /locus_tag="RHX88_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDV2231771.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8692..9930)
                     /locus_tag="RHX88_003110"
     CDS             complement(8692..9930)
                     /locus_tag="RHX88_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDV2231772.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10151..10579
                     /gene="uspG"
                     /locus_tag="RHX88_003111"
     CDS             10151..10579
                     /gene="uspG"
                     /locus_tag="RHX88_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDV2231773.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10700..12265)
                     /gene="ahpF"
                     /locus_tag="RHX88_003112"
     CDS             complement(10700..12265)
                     /gene="ahpF"
                     /locus_tag="RHX88_003112"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDV2231774.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12436..12999)
                     /gene="ahpC"
                     /locus_tag="RHX88_003113"
     CDS             complement(12436..12999)
                     /gene="ahpC"
                     /locus_tag="RHX88_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDV2231775.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13371..14117
                     /gene="dsbG"
                     /locus_tag="RHX88_003114"
     CDS             13371..14117
                     /gene="dsbG"
                     /locus_tag="RHX88_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDV2231776.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3366 a         3763 c         3582 g         3491 t
ORIGIN      
        1 cacgccgaaa tactccttag gatgtatagc gaaaggagaa aaagatatac ctcgatcacc
       61 cccctttctc ccaagtgaaa ataaaaggtt atcagtttgc aacattgaac aacattcgtt
      121 gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcggc aatgatattt
      181 tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa ttcctgcatg
      241 aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc cgcgatgaaa
      301 agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgct atcagtgaat
      361 ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac ctcgcctatg
      421 agcggcacag cacgcatctg tttatttata ccaaaaccga atacgaggcg ctgttccgcc
      481 agtgcggttt ttccacgctg accagcgtac ccggcgtgat ggtgctgatg gaaaacagcg
      541 ccacgcggct gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca gggaacaaga
      601 ttggctgcat tgtgatgaac gccaatccct tcacgaatgg tcaccgttat ctgattcaac
      661 aagctgcggc acagtgcaac tggttgcatc tgtttttagt caaagaagat tcttcacgct
      721 tcccctatga agaccggttg gatctggtgt taaaaggcac cgccgatatt ccacgcctga
      781 ctgtgcatcg cggctccgaa tacatcatct cccgcgctac gttcccttgc tacttcatta
      841 aagaacagag cgtcattaac cattgttaca ccgaaattga tctgaagatt ttccgtcagt
      901 acctcgctcc cgcgctgggt gtaactcacc gctttgtcgg tactgaaccc ttttgtcgcg
      961 ttaccgccca gtacaaccag gatatgcgct actggctgga aacgccgact atctccgcac
     1021 cgcccatcga actggttgaa attgagcggc tgcgttacca ggagatgccg atatccgctt
     1081 cccgggtacg tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg ctggtccctg
     1141 cagtcacgct gcattatttg cagaacctgc ttgagcactc ccgccaggac gcggcagctc
     1201 gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc ccgccgttgc
     1261 aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata cgcaggatat
     1321 cgacctgcaa atcaatagca gcgttgagaa acagtttggc gatgcaattc gcaccaccat
     1381 tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg atgacaaagg
     1441 cgcactggac tgcattttac gtgcacgact ggaagccctg ctggcacgcg ccagcggtat
     1501 cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca acgtaaaact
     1561 cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt cagcaactcc
     1621 ttcatctacc cggctgatgc cctgatgttt gacctcgaag actccgtagc attgcgtgaa
     1681 aaagacaccg cccgccgcat ggtttaccac gcgctgcaac atccgctgta tcgcgatatt
     1741 gaaaccattg tgcgtgtcaa cgcgctggat tccgaatggg gtgtcaacga cctggaagcc
     1801 gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc tcaggatgtt
     1861 ctggatatcg aaaaagagat cctgcgtatc gaaaaagcct gtggtcgtga acccggcagc
     1921 accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcagt ggaaatcgct
     1981 cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag aagactatgt gcgcaacctg
     2041 cgtacagaac gctccccgga aggaactgaa ctgctgttcg cacgctgttc cattttgcag
     2101 gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct attccgacgc taacaacgaa
     2161 gccggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg caaatcgctg
     2221 atcaacccgc gtcagattga tctgttgcac aacctctacg caccaaccca gaaagaagtg
     2281 gatcacgccc gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg cctcggcgtg
     2341 gtttccctga acggcaagat ggtggacggt ccggttatcg atcgcgcccg tctggtgctc
     2401 tctcgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga cgcagaaaat
     2461 tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcgctg aatgcgatct
     2521 tgccgctttc cagaactcgc caaagcaaac ctaccaggct gaaaaagcgc gcgatcgcaa
     2581 actgtgcgcc aacctggaag aagcgattcg tcgctctggt ttacaggacg gcatgacggt
     2641 ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga tggacgtcat
     2701 cgcgaagatg ggctttaaaa acctgaccct ggcgtccagc tccctgagtg attgccatgc
     2761 gccgttggta gaacacattc gccagggcgt ggttacccgc atttatacct ccggcctgcg
     2821 cggtccactg gcggaagaga tctcccgtgg tctgctggca gaaccggtgc aaatccactc
     2881 tcacggcggt cgcgtgcatc tggtacagag cggcgaactg aatatcgacg tggctttcct
     2941 cggcgtcccg tcctgcgatg aattcggtaa tgccaacggc tacaccggta aagcctgctg
     3001 cggctccctc ggctatgcaa tggttgatgc cgacaacgca aagcaggtcg tgatgcttac
     3061 cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc aggttgattt
     3121 gatcgtcaaa gttgaccgcg ttggcgatgc tgcaaaaatc ggcgctggcg cgacccgtat
     3181 gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga ttgtcaactc
     3241 aggctacttc aaagaaggtt tctccatgca aaccggcacc ggcggcgcat cgctggcggt
     3301 aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact tcgcccttgg
     3361 cggtattacc gcgacgatgg ttgacctgca cgaaaaaggt ctgatccgca aactgctgga
     3421 tgtgcagagc tttgacagcc atgctgcgca atcgctggcc cgtaacccca atcacatcga
     3481 aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggc gcatcggttg atcgtctcga
     3541 cgtggtggta ctgagcgcgc tggaaattga cacccagttc aacgttaacg tgctgaccgg
     3601 ctctgacggc gtactgcgtg gtgcttccgg tggtcactgc gataccgcga ttgcctctgc
     3661 gctttccatc atcgtcgcgc cgctggtacg cggtcgtatt ccgactctgg tggataacgt
     3721 gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc acggtatcgc
     3781 agttaacccg gcacgtccgg aactggcaga acgtctgcag gaagcgggca ttaaagtggt
     3841 ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc ggtgaaccac agccgattga
     3901 attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga tcgatgttgt
     3961 gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc accatgcggt
     4021 gtcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac acgtctggct
     4081 caagcgccat cctgttccac tggtctcctt taccgtggtt gcgcctgggc cgattaaaga
     4141 cagtgaggtc acacgccgaa tttttaatca tggcgtgaca gccttgcgtg ccttagccgc
     4201 aaaacagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg ggccggaggg
     4261 tatgttgagc attgccgccc cggctcgcga cctcaagctc gccaccattg agcttgaaca
     4321 tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag gcgaaattct
     4381 ctcccgccgc gactattcac tgccgcctcg cagctgcctg ttgtgcgagc aaagcgcagc
     4441 cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca tggaggcact
     4501 gctgaacgat gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg acgtcattaa
     4561 tcgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat ctgtcaccga
     4621 aaccaggcct cgtggatcgg attaactgcg gtgcgcataa agatatggcg ctggaagatt
     4681 tccaccgcag cgcgctggcg attcagggct ggctaccccg tttcattgaa tttggtgcct
     4741 gtagtgcgga aatggcacca gaagcggtac tccacggatt acgcccaatt ggtatggctt
     4801 gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac gcataaaggc agcatttttt
     4861 ctttagggct gctatgtgcg gcaattggcc gtttgcttca gctcaaccag ccggtaacgc
     4921 caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg tctgaccgat cgcgaactgc
     4981 gtaccaataa ttcacaactg acggcaggtc aacggttgta ccagcagctt ggccttaccg
     5041 gcgcacgcgg tgaagccgaa gcgggttatc cactggtgat caaccacgcc ttgccgcatt
     5101 acctcactct gctggatcag gggttagatc ctgaactggc attgctcgat accttgctcc
     5161 tactgatggc gatcaacggc gataccaacg ttgcttcgcg cggtggcgag gggggcctgc
     5221 gctggctaca gcgcgaggcg caaacattat tgcaaaaagg gggcattcga acccccgccg
     5281 atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatctc agtccaggcg
     5341 gcagtgccga cctgctgatc cttacctggt ttttagcaca gatttaatta tttaagcact
     5401 tgataaattt gaaaatatta attttcggag aacccgtatg tctttagcaa aagataatat
     5461 atggaaacta ttggccccac tggtggtgat gggtgtcatg tttcttatcc ctgtccccga
     5521 cggtatgcca ccacaggcat ggcattactt cgctgtgttt gtggcaatga ttgtcggcat
     5581 gatcctcgag ccaattccgg caacagcgat cagttttatt gcggttacta tttgcgttat
     5641 tggcagtaat tacctgctct ttgatgccaa agaattagct gacccagcgt ttaatgcgca
     5701 aaaacaggcg ctgaaatggg gtctggctgg tttttccagc accacggtat ggctggtatt
     5761 tggcgcattt atttttgcat tagggtatga agtttccggg ttaggtcgtc gcattgccct
     5821 tttcctggtg aaattcatgg gcaaacgcac gctgacgttg ggttatgcga ttgtcattat
     5881 cgacattctg ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg gtacggtttt
     5941 tccggtcatt aaaaacctgc cgccgttgtt taaatcattc cccaacgatc cgtccgcgcg
     6001 tcgtattggc ggctatttga tgtggatgat ggtcattagt accagtctga gttcgtccat
     6061 gtttgtcacc ggtgcggcac caaacgtgct gggtctggag ttcgtcagca aaattgccgg
     6121 tatccagatt agctggttgc agtggttcct ctgcttcctg ccggttgggg ttatcttgct
     6181 tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac acagtgaaga
     6241 agtggcaacc tgggcgggtg atgaactgaa aaccatgggt gcgctgacac gcagagagtg
     6301 gacactgatt ggccttgtat tgctcagctt aggtttgtgg gtttttggca gtgaagtcat
     6361 taatgctact gcagttggtc tgctggcagt ttcgctaatg ctggccctgc acgttgtgcc
     6421 ttggaaagat attacccgct ataacagcgc atggaacacg ctggtcaacc tggcaactct
     6481 ggttgtgatg gctaacggcc tgactcgttc tggctttatt gactggttcg ccggtaccat
     6541 gagtacgcac ctggaaggat tctcaccaaa cgcaacggtg attgtactgg ttctggtgtt
     6601 ctactttgca cactacctgt ttgccagcct gtctgcgcac accgcaacca tgctgccggt
     6661 tattctggcc gtcggtaaag gtattccggg cgtaccaatg gaacaactgt gtatcctgct
     6721 ggtgctgtct atcggtatca tgggctgtct gacgccgtat gcaaccggtc ctggggtgat
     6781 tatttacggc tgtggctatg tgaaatcaaa agattactgg cgtcttggcg caatcttcgg
     6841 ggtgatttac atctctatgt tgctgttggt tggctggccg attctcgcca tgtggaacta
     6901 atacgttcgc caaacgctga ataaatcatc acgcccgcca ggttagccca cctggcgggc
     6961 ttttttatga tttaatagat agtattcagt gtatgtatga gttccacacc cattatgaaa
     7021 gcattctggc gtaacgccgc gttgctcgcg gtttctctgc ttcccttctc ttctgccaac
     7081 gccgtagcgt tgcaggcaaa acagtatggc gattttgatc gctatgtcct ggccctctcc
     7141 tggcaaaccg gattttgcca gagtcaacac gatcgaaatc gtaacgaacg agatgaatgt
     7201 cgcctgcaaa ccgaaacgac caacaaagct gattttctga ccgtacatgg tctgtggcca
     7261 ggattgccta aatcggttgc tgcccgtggt gttgatgaac gccgctggat gcgcttcggt
     7321 tgcgctactc gcccaatccc gaatctacca gaagcacgcg ccagccgaat gtgttcatcg
     7381 ccggaaaccg ggttatcact ggaaacggcc gctaaactaa gtgaagtcat gccaggagct
     7441 ggcggacgtt cctgcctgga acgctacgaa tatgccaaac acggtgcctg ctttggtttt
     7501 gatccggacg catacttcgg tacgatggta cgcctgaatc aagaaattaa agagagcgaa
     7561 gccggaaaat tccttgcgga taattacggt aaaacagtga gccgccgtga ctttgacgcc
     7621 gcctttgcca aaagctgggg aaaagagaac gtgaaagcag ttaagctaac gtgccagggt
     7681 aaccctgcgt atttgactga aattcagatc tcgatcaaag ctgacgccat caacgctccg
     7741 ctttctgcaa actcattctt gccacaacca cacccaggca actgtggcaa aacctttgtg
     7801 attgataaag tgggttatta atcatctgat ttacaaaaca cgctaattcg tcttccataa
     7861 accgtgacga ttaagaatta tacataatgc gctgcattga ggaattccct gaatgtgacg
     7921 caaatcactt caaagagtga gcaacagtca gtatcatcat gtgagttaaa ccctcgccgc
     7981 ctgacggtga gggttttctt ttgggtatgt ttatctgcga cactcgcagt accgacgaca
     8041 tggagtaaaa atgtccagac caactatcat cattaacgac ctggatgccg aacgcatcga
     8101 tattctgctg gagcaacccg cctatgctgg tttgccaatc gccgacgcgt taaacgcaga
     8161 gttggatcgc gcccaaatgt gttcgccaga agagatgcca cacgacgtgg tgacaatgaa
     8221 cagccgggtt aaattccgca atcttagcga tggcgaagtg cgtgtgcgca cgctggtgta
     8281 tccggcaaaa atgaccgata gcaatactca gctttccgtt atggctccgg taggtgccgc
     8341 actgctgggg ctgcgcgttg gcgattccat tcactgggaa cttccgggcg gcgttgcaac
     8401 ccaccttgaa gtgctggaac tcgaatacca gccagaagct gctggcgact acctgcttta
     8461 atcccatctg cacaagcgat aactcgcttt gcccagagga tgcactcgca tcctctttac
     8521 gcaaaatcag caatttttcc tggttctcac tgttcacttt tgctgcaaaa ttattccgtt
     8581 tcgtttgtgg gaataaccat ggcagaagca ttttacatcc tgataggatt tctgattatg
     8641 gcggcgatta tcgtcatggc tgtcctttac ctcgaaaacc actcctgacg atcatattgt
     8701 tccccccggc atcgcattca ccagacctga aaccgccttc tgcgccgcct ctgcgctttg
     8761 tgcaccgggt accagaatca ccttacggca ctcctcttca cgtttttcga aaatctcata
     8821 tccccgggcg gcctcttcaa acggcatata gtgggtaaca atttcttctg gtttcagtaa
     8881 tcctttctca attaacggta ataattctcc cagccatgcg tgaacgtggg tctgtcccat
     8941 tttaaacgtc aaccctttat caaaggcgtc gccaaacagg aaaccgtgaa taaatccagc
     9001 gtagacgccc ggtacgctaa caatgccgcc acgcctgacc gccgcaatac actggcgcaa
     9061 cgctttaccg ctgctgccct ccagtttcag gttagtcagc accgtttccg tggtgctgcc
     9121 tttcgcttca aaaccgacgg cgtctattac tgcatccacg ccccggtgac ctgccgtttg
     9181 ttcaataatt gactgtgccg gatcgctatc ttcatcgaaa ttaatcggga tcgcgccgta
     9241 gcggtcagcg gcgaaacgca agcggtaggg atgatgatca acaacaaaaa tctgttccgc
     9301 accgagcaac cgtgcacagg cgattgtcaa caatcccact ggaccggcac catagactgc
     9361 aacgcttgaa ccttgttgga tctgcgcatt ttttgctgcc tgccatgccg ttggcagaat
     9421 atcagaaagg aaaagggctt tatcatctga aagcaaaggc ggtactttaa acggccccac
     9481 attcccttta gggacgcgga catattccgc ctgtccacca ggaacgccac catacaggtg
     9541 actataacca aacaatgccg cgggagctgg tatctgtttt ttattgagcg cagcgccttt
     9601 acccgcattg gtattttcgc aggcggcata ttgctgcatt cgacagaaaa aacagtcgcc
     9661 acaagcaatg acaaacggaa ttaccacccg gtcgcctttt tgcaaatttt ttacgtcctt
     9721 tccggtttca acgacttccc ccataaattc atgaccaaaa atatcgccat gtttaacctg
     9781 gggtattttg cctcgataaa gatggaggtc agagccacag atcgccgttg ccgtaatacg
     9841 cagaataata tcatctgcct gttcaatgcc cggatcggga acattttcta cctgaacgtg
     9901 atgtgggccg tgataagtca atgctttcat gcaacctcca gagacagttc aaaaaagagt
     9961 cagtctttaa gggtagctat cacagcggga aaacgggcgg taccgcaggt tttgggagaa
    10021 ttcctggtgt tcagtgagaa tatattaatc acagaaaccg ttcgtaagga aaaagaacca
    10081 gacaaatatc agggataaat attataatca tggcgaatga tttgttcatg attaacaggg
    10141 agaaagggtt atgtataaga caatcattat gccagttgat gtatttgaaa tggaattgag
    10201 cgacaaagct gttcgccacg ctgaattcct cgcccaggat gacggagtta ttcatctact
    10261 tcacgtacta cccgggtcag ccagcctgag cctgcaccgt tttgccgctg atgtgcgtcg
    10321 ttttgaagag catctgcaac atgaagcaga agaacgtctg caaacgatgg tcagccactt
    10381 caccatcgat ccttcccgca ttaaacaaca tgtccgtttt ggtagcgtgc gggatgaagt
    10441 caatgagttg gcagaagaac tgggggctga tgttgtagtt attggttctc gcaacccatc
    10501 gatttcgacc catctgttag gttctaacgc ctcgagcgtg atccgccatg ccaatctgcc
    10561 ggtgctggtt gtgcgttaat tgccagaata aatatcccgc cctgcccgga gccatcccgg
    10621 cagggctttt taatgccata aaaaagccgc caggtttgac cccggcggct aagcaattgc
    10681 aggtgaatct tacttcttct tatgcagttt tggtgcgaat caggtagtca aaagcactca
    10741 gagaggcttt ggcaccttcg ccagtggcga tgatgatctg cttgtacgga accgtcgtac
    10801 agtcacccgc tgcgaatacg cctttgacgt tggtttcgca tttcgcatca atgataatct
    10861 cacccatgcg gttacgttcg actgcgcctt ccagccagtt ggtgttcggc agcagaccaa
    10921 tctggacgaa aataccggcc agttcgatgt tgtgaatatc gccgctgaca cgatctcgat
    10981 attccagacc aacgactttg ctgccgtcgc ctttcacttc cgtggtttgc gcattcagaa
    11041 taatgtcgac gtttttcagg ctgcgcagtt tgtcctgcag aacctggtcg gctttcattt
    11101 ctggcgcaaa ttcgagcagc gttacgtgct caacgatacc cgccaggtca attgccgctt
    11161 ccacgccgga gttaccgccg ccgataaccg ctacgcgttt gcctttaaac agcgggccgt
    11221 cgcagtgcgg gcagtaggtc acgcctttgg tgcgatactg atcttcgcca ggaacgttca
    11281 tgttgcgcca ttttgcaccg gtcgccacga taatgctgcg tgctttcagt accgcgccag
    11341 aagctgtttc aatctgatgc agaccacctt caactgctgc tgggatcagt ttgctggcgc
    11401 tctggctgtc gatcacatca acgtcgtatt catcaacgtg aactttcagt gcgcctgcca
    11461 gcttctgccc ttcagtcttc ggtacagaaa tgtagttttc gatatcaacg gtatcgagga
    11521 tctgaccacc aaaacgttcg cccatcagac cggtacggat gcctttacgt gcggagtaaa
    11581 ttgctgccgc tgcacccgcc gggccggaac caacgattaa tacgtcataa gcatcacgct
    11641 tgttcagctc ttctgccgca cgtttttccg cgcccgtatc aattttggca acgatttcag
    11701 tcaacgtcat gcggccctga ccaaactctt tcccgtttac gaacactgcc ggaacgccca
    11761 tcacgttgcg atcggtgatt tcgttctgga aggtgccgcc gtcaattgca gtgtgcttga
    11821 tgcgcgggtt cagtacgctc atcaggttca gcgcctgcac cacgtccggg cagttatggc
    11881 aagagagcga gtaataggtt tcgaattcaa aatcaccgtc aatatggcga atctgctcca
    11941 gcagagactg cgcttctttc gacggatgac caccggtcca cagcaacgcc agtaccagcg
    12001 aggtgaactc gtggcccagc ggggagcctg caaaacgtgg cccctggttg gaacctgggt
    12061 tggtgatcag gaaagacggc ttacgcaccg gcaagctgtt atcttcttta aaggtgactt
    12121 tgtctgacag ttctgcgatt tcagccaaca gttccttgat ttctgccgat ttagcgctgt
    12181 catccagcgt ggcaattaac tcaacaggct tggtcaattt ctcaaggtaa gccttgagtt
    12241 gagttttcat atttgtgtcg agcatgttta tctcctgggc tttaaacatc atcatgcaag
    12301 cggcctctgc ggctacctgg atgcagcttg catcatggtg cggggaagaa aatcgggtgc
    12361 cgcagcaccc gaagaattag gtgaatttcc gaagtatttc gcgccggagt tggtagcgcg
    12421 aaagacgaag gaaatttaga ttttaccaac caggtccaga gacggagcca gagttgcttc
    12481 accttctttc catttagccg ggcaaacttc acctgggtga gaagctacgt actgtgctgc
    12541 tttgatttta cgcagcaggt cagacgcgtc acggccaatg ccttcagcgg taacttcgat
    12601 tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca cggtcagcca gaccttcatc
    12661 ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc gggtcgccga tcatcgcata
    12721 tttgatttta gcgatggttt cagagctgct gtgccatgct ttgtgggtga agtgagtatc
    12781 ggtagatact gcgtatacgt ctacgcccag tttctgcagt tcttcgtagt ggtcagcaac
    12841 gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc gggtagaaga agaagacgct
    12901 ccagcggcct tcggtatctt tttcggtgat ttcgatgaat tcgccgtttt tgaatgcctg
    12961 gtttttaaaa ggtttaattt tggtgttaat caaggacatc tatacttcct ccgtgttttc
    13021 gatgagatgt aaggtaacca atttttgccg attcggctaa tgcgtttcca ttatcaaatc
    13081 gataagtttt accttacaac cttcgtaaga caactttgtg aactttgcac cgccagcggc
    13141 gataaaaagt aaaatggccc cctttccagg tgaccgtgta atctgaacta ccctttggta
    13201 tcttcagcgc ctgcctgcag gctgaggtgt tgcccttaca aatgcaacaa cgacatggat
    13261 tacaacaccc tcataaacaa agggcaatca cctgatctaa gctcttacct atgacagtga
    13321 taggttatgc cttttactcg acttttgcac tgactgaaaa ggacaaatta atgttaaaaa
    13381 agatactttt actggctctg cttcctgcaa tcgccttcgc agaggaactt ccttccccag
    13441 taaaagcgat tgaaaaacag ggcattacaa tcatcaaaac gttcgatgcc cccggaggaa
    13501 tgaaaggtta tctcggaaag tatcaggata tgggcgtcac catctacctg actcccgatg
    13561 gtaagcacgc tatctctggt tacatgtaca acgagaaagg tgaaaacctg agtaacacac
    13621 ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat gtggcaacgg atggaacaat
    13681 cccactggct cctcgacggt aaaaaagatg cgccggtcat tgtctacgtg ttcgccgatc
    13741 cgttctgccc atattgtaaa cagttctggc agcaggcgcg cccgtgggta gattctggca
    13801 aagtgcaatt aagaacattg ttggttgggg ttatcaagcc agaaagcccg gcgacagcag
    13861 cggcaattct tgcctccaaa gatcccgcaa aaacctggca agaatatgaa tcctctggtg
    13921 gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga acaaatgaaa gtgttaagcg
    13981 ccaacgagaa actcatggac gatctggggg caaatgtcac accagctatc tattacatga
    14041 gtaaggaaaa tacgctgcaa caggccgtgg ggttgcccga tcagaaaacg cttaatatca
    14101 ttatggggaa taaataaaag ataattcatg gatggttgaa atacatccct gcttttcaac
    14161 catcctaaaa aagcatgaat tgctcgctct gccctaagtg gc
//
LOCUS       DAPGWD010000107        14070 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPGWD010000107 DAPGWD010000000
VERSION     DAPGWD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.108"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(65..412)
                     /gene="trbA"
                     /locus_tag="RHX88_003115"
     CDS             complement(65..412)
                     /gene="trbA"
                     /locus_tag="RHX88_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HDV2231777.1"
                     /translation="MSEDYLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMSQGAALVVSIVAAALPDVTSFDRFRRIFICIVLSSVWS
                     GVMWFFIRGLMTG"
     gene            complement(428..1171)
                     /gene="traF"
                     /locus_tag="RHX88_003116"
     CDS             complement(428..1171)
                     /gene="traF"
                     /locus_tag="RHX88_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HDV2231778.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(1164..1421)
                     /gene="trbE"
                     /locus_tag="RHX88_003117"
     CDS             complement(1164..1421)
                     /gene="trbE"
                     /locus_tag="RHX88_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HDV2231779.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYYGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(1448..3256)
                     /gene="traN"
                     /locus_tag="RHX88_003118"
     CDS             complement(1448..3256)
                     /gene="traN"
                     /locus_tag="RHX88_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HDV2231780.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGGDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFNRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(3253..3891)
                     /gene="trbC"
                     /locus_tag="RHX88_003119"
     CDS             complement(3253..3891)
                     /gene="trbC"
                     /locus_tag="RHX88_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HDV2231781.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RTVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(3900..4892)
                     /locus_tag="RHX88_003120"
     CDS             complement(3900..4892)
                     /locus_tag="RHX88_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HDV2231782.1"
                     /translation="MKRRLWLLMLFLFAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(4889..5521)
                     /gene="traW"
                     /locus_tag="RHX88_003121"
     CDS             complement(4889..5521)
                     /gene="traW"
                     /locus_tag="RHX88_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HDV2231783.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPILESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(5518..5904)
                     /gene="trbI"
                     /locus_tag="RHX88_003122"
     CDS             complement(5518..5904)
                     /gene="trbI"
                     /locus_tag="RHX88_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HDV2231784.1"
                     /translation="MSSTQKPADVTAERRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRVEP"
     gene            complement(5901..8528)
                     /gene="traC"
                     /locus_tag="RHX88_003123"
     CDS             complement(5901..8528)
                     /gene="traC"
                     /locus_tag="RHX88_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HDV2231785.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTSAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(8688..8909)
                     /gene="traR"
                     /locus_tag="RHX88_003124"
     CDS             complement(8688..8909)
                     /gene="traR"
                     /locus_tag="RHX88_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HDV2231786.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(9044..9559)
                     /gene="traV"
                     /locus_tag="RHX88_003125"
     CDS             complement(9044..9559)
                     /gene="traV"
                     /locus_tag="RHX88_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HDV2231787.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(9556..9807)
                     /gene="trbG"
                     /locus_tag="RHX88_003126"
     CDS             complement(9556..9807)
                     /gene="trbG"
                     /locus_tag="RHX88_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HDV2231788.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            complement(9819..10049)
                     /locus_tag="RHX88_003127"
     CDS             complement(9819..10049)
                     /locus_tag="RHX88_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HDV2231789.1"
                     /translation="MTEQTETTEIKMNMRNINVITALSVPGKTVSDDFMHAVLSNCAK
                     RIVLPAPEKFGSESLPDNFNMTAVGVMKNSEI"
     gene            complement(10003..10593)
                     /locus_tag="RHX88_003128"
     CDS             complement(10003..10593)
                     /locus_tag="RHX88_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HDV2231790.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            complement(10583..12010)
                     /locus_tag="RHX88_003129"
     CDS             complement(10583..12010)
                     /locus_tag="RHX88_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HDV2231791.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVLQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(12010..12714)
                     /gene="traK"
                     /locus_tag="RHX88_003130"
     CDS             complement(12010..12714)
                     /gene="traK"
                     /locus_tag="RHX88_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDV2231792.1"
                     /translation="MLGGLFFSCSVMAGVGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQVVRGGKLPAGWYQVPVTKETLQ
                     TPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            complement(12725..13291)
                     /gene="traE"
                     /locus_tag="RHX88_003131"
     CDS             complement(12725..13291)
                     /gene="traE"
                     /locus_tag="RHX88_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HDV2231793.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETADASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(13313..13624)
                     /gene="traL"
                     /locus_tag="RHX88_003132"
     CDS             complement(13313..13624)
                     /gene="traL"
                     /locus_tag="RHX88_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HDV2231794.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPNSCFRQWI
                     K"
     gene            complement(13639..14004)
                     /gene="traA"
                     /locus_tag="RHX88_003133"
     CDS             complement(13639..14004)
                     /gene="traA"
                     /locus_tag="RHX88_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HDV2231795.1"
                     /translation="MDAVLSVQGVSAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
BASE COUNT         3225 a         3983 c         3409 g         3453 t
ORIGIN      
        1 atcatatagt cttcagtaat caacctcatt attttgtatg gtttttatga ccgttttata
       61 tgacttagcc tgtcataagc ccccttataa aaaaccacat cactccggac cataccgatg
      121 acaacacaat acagataaat attcttctga acctgtcgaa gctcgtaacg tccggtaatg
      181 cggccgcaac tattgaaaca accagtgctg cgccctgact cataattgca ccacacagaa
      241 accagagact ggcggtaagc acatataaac acgcgatatt aataacaagg aaaaccagtg
      301 agctcaggag aaacattttc ctgtcattac gctcagaaaa gaaataaccg gcaataatga
      361 caaatattaa cagaacaaca cctgtaaaca ttttcaaata atcttcactc ataaatcaaa
      421 tccgcgatta aaaattgggt ttaaaatctt cagaaacgtt caggaactgt tttgccaggt
      481 cgtcctgcga aataaagccg tatgataacg ggcgaacact gccctgtttc gggtcaacca
      541 gcatcatggc cgggaaatat ttcacgccga ggcgctgcgc ctgcccctgg tcagtccggg
      601 aatccggcaa cagcggatta atcacgccat ccacggaaac gggaataaca ctcagaccat
      661 acgtatcccg gaagccatta atgacctgcg ccagttgccc gtcgatgggg tcctgccccc
      721 ggtaaaaaaa catgatgccg tagtgttcag ccagtttcgc aatggcctgt cgctgctgcg
      781 cctggtctgc tgccagctgg ttccgtaccg tgccgttgta atggctgtac tgcaggttat
      841 agtccagttc aggatgtgcc agcatggcct ttctggcgct catggtgaaa agcccggcct
      901 gctgagtcca gtaattttgc agccggaaat atttcacaaa attatccacg ccgggataca
      961 gaatggcttc gtacagcgcc cgctttgttg ccgtctgcag tgcggccagt ttctgcataa
     1021 tatccggttc ctgacggggg gctgcaggta caggtttatt ttctttttcc ttcggattta
     1081 ttttctcgtt ataccactgc cagcctgcat cttttcctga cgccggaaaa ataaagcagc
     1141 agagtaacag tggcagtaat gctttattca tcttcattcc tttcatcact ttttctttca
     1201 tcaggtttct gcaggtcagt cagggcagaa aaacaaaaat acagaataaa caggagcaca
     1261 ccgtagtaaa ggataacgaa agcagcggca agcatcccgt ctgccgtggt ttcttttacc
     1321 gtatcccacg accagataat taccggagaa atgacaatcg ctgtaatcaa aagccggata
     1381 agaaaatcaa taaaacggtt cgacgtgaat atgactttca tcatttcccc cggttatttc
     1441 ccatacctta ctgtcctgcc tgtttcagct ggtcagcaat ctgctctttc actttctgcg
     1501 tcagaacgcc actgtccggg attttctggt tattcatcag gtcttcgtag aagttagtga
     1561 aatccagtct gttgaactga attttctgca gctcatcaac cgtaatcccc cgacagtcag
     1621 gatgcttcgc actaccaaaa ctgatacgca actgaccgtt gcgcccctgc tgctggacta
     1681 tctgcgccag cttcgaatca aactgacagt agctgcgttt tttctccaga cagacaccca
     1741 gcactttctt cgaacagaac tccccgacgc tgacggtcag tttgtttgat tttgctttag
     1801 ccagggcctt ttcatcactg ctgcatttgg ccagcccgat atcctgtccc cagccgctgt
     1861 ccttacagca gttgctgtat ccggctgcag ccttcttaca aaatttcgcc tgaccggtga
     1921 aagcacggac atcccccccg ttcagcgccg caacatcctt acctgctgcg gccagtgccg
     1981 ccagctgaga aacggcttcg gcaaaatcat tactctgccc gctctgagcc ttatcgcatt
     2041 cgccatcgag acagaatacg tctcccccac agaccatcac tttcccggat gtttttgact
     2101 cacaggagta cgtggcatat tcatgcagac aggcaccttc ttcggagtaa aaggcacact
     2161 ggtgtgacac cagggtacag gcaggattgt cggtgtaggt ctgacaggtt cccttgtctg
     2221 ctgactgcgt gacatacgtg tcccggtatg cccagcagct ctggtacatg ctgtatgact
     2281 gaccgtcttt caccagcgtc ctgttcccgc cagcctccgt acattctgtt tttgtcagtt
     2341 tccccagctc cttgctgaac gggcagcttt cagaccagac aacatacggg gtgtacgagg
     2401 ctttttttac cctgacaacc agacgcagct tcattgccgt atgacgtttg aattttgtat
     2461 tcccgacccg gacggggtga gatgacgtaa aagccacacc ggcagtcagt gttttctgta
     2521 ctggtgtaaa tgaggcatct gctgagtatt tggcattcca gctcagtggg gttcccagta
     2581 ctgtaattgt cataggtaca ctcttctgca agtaagtacg actccagctg taatgcgcat
     2641 cgacaatttc accggtaacg ggagaggtaa tggagacaac atactgacca ttcacttcac
     2701 gtgcaggtag ctgactcatt tcatactcca gcgtccgggt ttcccatgtg gtactcccct
     2761 gcagctccat acgggcagtt cgtgtgcagt actgctccac ctgtaaatcc cgctcacagg
     2821 tgtaattcgt gtattcactc ctgctaatct cctgcgcact gcactgctgc ccggtgtttc
     2881 ccacaatgct gtcagcccgg ttcaccacat ccctgccggt ctggataaac ggtgcatccg
     2941 gtgaaagaat gtctttcggc ttgttcatga atgactctgt aatggttttt ccggtttcac
     3001 cggtcgccca ttccgtggtg ccgtcatttt tcaggccgcc atccccgccg gcagtcacgc
     3061 caccgtaata ttttgtctcg tccggattcg cgttatagcc ggggatactc tcctgtggct
     3121 taaagccctg aatactgctg cttccctgcc ctttgatctg gtgagcaaaa tcagaaccgg
     3181 cccggtaatc actgttgctg tcagcctgtg ccatgccggc aaccagagcc agtatcagag
     3241 gtaaaatacg tttcatttcc cggaatctcc tttccctgcc agtaaatcat gagccacctg
     3301 ccggcagtca cccgtggcgg ccactttctc cagcgcctgg ccgacccgca ggtttccccg
     3361 gatgatgtcg tatccctgac tgcagaacac caccagtgcc ggtacagtgc gaatgccgta
     3421 ctgtgaaaac agcgtcgggt cgatctgaac accatcagtc gcgccgtctt tcaccaggga
     3481 caacacagct tcagcggtgg tcttcaggtc attgttcacc atgccccgca gtgtggccgg
     3541 aataccgtag tgccgggttt cgcccagcat tcgtttcagc ccctcttcgg gaatggaaaa
     3601 cgacacaaaa tacagagcac cctgccgggg tttcccgtcc tgcgaagcct gctgtttacg
     3661 aaccagctca tccaggaaat ggttatctga acgctgaagg gggttttcca gcacctgttt
     3721 ctccgcccag gctttcagct gatggtcagg tttttcacgc agttgtctgc ttaaattttc
     3781 ctgctgcttc aggaactggc ggttttcagg agtgttcacg ttttctgacg ccataaccgc
     3841 cccgttcagc atcatcagca gtgctgccag agatttcata ctcagcttca tcattcacct
     3901 cacaggaaga cacagttacg tttacgccac atcaggtagc cgaagttttt ctttgtgttg
     3961 ggcggatttt tcccggtttc ccagcgggtc acgctgcgcc cgaacgggtg gcactgcccg
     4021 ctgtccggat acatattcac catctggtaa cgccagcgtt ctttgggcag gattggggac
     4081 ggatattcat tacagacggc gttatttttc ccgatggttt ccataatcat gccctgacgg
     4141 tgcagcttga acgccatgcg ttcactgacc agcagggagg actgcaacgg actggactca
     4201 ttactcaccc agccattgaa cgggtacata cttccctgcg aaccggcaca ccagaacaga
     4261 acatcgagag gcatattaaa ggcgctggca atcgcatctg ctgcgcaggc tccctgtgct
     4321 atcggattgg caaagatgac agcttccgga ttgagaatgg tggtcaggct gctgtccgtc
     4381 caggtggggt cgatttcaga aagataagcg atatccaggt caccaccttc cagacagccc
     4441 agcgatgtga tgatgttcag ccagtacgtc agcgggtatt tgtaccagtg aacgtgatag
     4501 aacgccccat ttacctgctt ctcatccttt ttcgccgttc cctgtgccgt tttaccaaaa
     4561 gccggcaggc tgaagcccag gttcaccatg catcccggtg accgggtgac gtccgtcaac
     4621 gccatcggct cccagtaacc aatggccagc ccgatacgcc tgaacagcgg cggcggtgcc
     4681 ggacaaatct gaatgggcat cgacgggttc gccgtgtcgg ggaccttacc ctgactgact
     4741 ttgatactgc ccagcgagag cgggaaaata cagctccagc agatatctgt gatcgggttt
     4801 acaaaacgcc cctcacaggc agaatccgca gacgccgcag ggacatgacc ggcgaaaagg
     4861 aataacatca gcagccacag ccttcgcttc attttctgcc ctcctctgcc ggaataaatt
     4921 ccaccttcag gaagcgatcg ccgggaacgg cgctcacccg tgccgggacc tgatcaatgc
     4981 cgaggcgctg gcagagcaca ccgttctggt caaagtaaac acggctgtcc agagacttct
     5041 gcatctccgg gatactgccc tgcacgagga taattttgct ctccagtatg ggcggcgtct
     5101 ggcgtttcat ccaggccacc tgcgccggat catcgccatt gatgaaatac agcgtctggt
     5161 taaaaggaac atactgcagc ggattcatca cttcaccctg acgggcaaac acccgccctt
     5221 cgttatcccg gatatccgca gccagtctga cggacgggtc aaacaaccgg ctgccgtatt
     5281 tctccgtgcg tccgataccg ggcacggcag ggggccgcag actgttgcgg atcacccgct
     5341 ccttaaaggc atccattttc cggcccatct caccggactg ctccagtgcc gtcaggcgct
     5401 gcatgatcac cgtcagcatg tccggctcct ttaccggcca cagatcgccc caggtaccaa
     5461 gatcggcggc ggccacactc tgcccccata tcagcagggc aatcagcccc cggcatctca
     5521 tggttccacc ctcattcgcc gggcaatatc ctgctggatt tcccgggtga tatccggtgc
     5581 cccctgtacc acagcaggcg acaccagaat gaccgcgtgg tgtttctgct gccacgcctg
     5641 caggctggct tccagtgccg tgttaaaacg cgctgaaagg gcttttgact gcgcttctga
     5701 cagttgtttc tgactggcgc tgtcaaaaaa cgcatccacg gtctgtttca tgttgaaggc
     5761 gacagtcacc ggtgcgttca gtctgacaat gccgtaactg acggctgcgt tcagtaacac
     5821 cattgccagg catcccggca ccgtccacca ccagtggctg cgtcgttctg ccgtgacgtc
     5881 ggcgggtttc tgcgttgaac tcatgccaca ctcctgtatt tctcatgttc ttccagccag
     5941 gcttccagcg aagccatttc cggccctgac ttcttccacg ccagctgcca cactgcctca
     6001 tgaatactca gcccctcttt acgcttctgc tgcacaaact caaaatccgg gccgtcagag
     6061 ctgtacatgg cgcggcttaa cgggtccaca aacagacggt gccaggagga atggttttcc
     6121 acctgcagca ggaaggaact gaaccactgg tctttggccg caccaaactt accaatcatg
     6181 tcgcgctgca gtggcaggaa ctggtccgga tacagttggt tgtatttcgc gaactccttc
     6241 gcactctgtt tgaggataat tttgtaggag gagttacccc atgccgcgcg ggcagcactg
     6301 gaggccttat cagagtcaaa gtcgacgatg ttctgtgtga tggtgatata ggcaccggta
     6361 tgacggcggg ccgtacggta gcccttctca ataaattcac cgaccttgtg atttttgaag
     6421 tccagcagac gccagccttc atcaataacg ttcagtttct tgaggttacg cggcgtgcgg
     6481 tacatcctgt tctcgatgta gataatcagg gagaacatca ccgcaaccag cagtgacgga
     6541 cggtcttcca gtccgcccag ctccagcacc accatttttg cgtcatcccg cagggacggc
     6601 tcatcagagt taaaatactg gccgtaagtg ccgttggcag tgtactggtc aagcagcaca
     6661 atcatttcgt ccagacggct gcggatagtc ggtgactcgg catactgctc gctgtcactg
     6721 gcgtttttca ggaaatccac cacgtcatca atacgtgctc tgttctcttt ggccagccag
     6781 gaggctctga ccgcctgcaa cagcagacct tcatgcactt catccaggtt accgttgggg
     6841 ctggccatca ctgacaactg gtcacggaca cgctccgctg actggtcaat atcggtgatg
     6901 ttcgcaaacg ggttaaaacg cagggtttca ccgtccagat acaccccgcc catgttctca
     6961 cacagggact tgtatccatc ccccatgtcg aacaccacgg caaagcctcc ggagtccagc
     7021 acgctgcgga taagtggctg tatcagcccg gttttaccgg ccccggaggt gccacagacc
     7081 gccatgttgt agttggtgtt attcatcccc cggaagaaaa tatcgataaa cgccagctgg
     7141 ttacggtagg tgggtgccag cagacctgcc gatgtcaggg ggttatccgc cactaacggc
     7201 atcaggttgg caacattaaa gctctctgcg cgctgaacca ctccggcttc tttcagctgt
     7261 ttaaacagcc ctttcccggc cataaagggc aggcaggtca ggaaattgcg catgtggtta
     7321 aagcgcggtg aaatcagctc aaaaccgttt ttacgaaagc tgttcaggat gtcctgctcc
     7381 acttccagcg ccgtttcatt attgtccttg cagaatgctg tgatgttgag gaagtaggac
     7441 accacggagg actgaccgga gcccagccgc tgacgcagtt ccccccactc cttcgcctct
     7501 ttctccacgg acggaaacca tttggcatag gaggtcttcg actttttctc caggtccatg
     7561 tacttcaggt tggcttcgct gtgggttttc acctggtctt ccaccaccag agtcagcgtc
     7621 aggatgaacg gacaggagat ggacagttcc gggttcagca ggttgctgta gttgtcggcc
     7681 acgttccaca ggaaggcgat ttccgggtta cgggccagat ggaaattcag gatgcgggcc
     7741 gtgctgttcc tgccgttctc acgcaggccc agcgtcaggt aatcagcccg gactttcaga
     7801 tcaaaactgt cctccacaca ctgataattc agatcagaat acgggtccag ctgacgtctt
     7861 ttcgggtaca gggagtccgg gttatggtta atcatctccc cgacaatatc gataaaggcc
     7921 tgtgcatcca ccgtctgtgt ggtgatactg gccccctgta acgacgcccg gatgattttc
     7981 accaggtttt ccatttccag aatgtcggcc cggctttttt tcttcgaagg agaacagtac
     8041 gagataaaca cccggtaatg gcgcagggtc aggggcagat tcatcccctc cggcagcgga
     8101 aactgtgtcg ctgccgcttt catataatag gcccgggtaa tggcgttaaa ccgttcagcc
     8161 tgttcacctg accaggagaa ctcacgcagc ccgtattcaa tcctgtcacc aacaagctga
     8221 ctggacatca gatggataca caacggaata ccgcgcggca gtttggtgcg cagcatgtga
     8281 tcgagggcct ccacaatgga ctcattcgcc ccattgatgg ggatggcttc cagcataaag
     8341 cccatcgtgg tgtcattcat gaacagaccg gattcctggt tgtaatcacg gtacggcagc
     8401 agacggctga actgcgggaa gctcatttcg cccagaactt cattggcctt tgcggattcg
     8461 tcaggcagtt tcagtgctgt gacgagggag ttaaccgcct gagtgacggc ctcaagtggg
     8521 ttattcacat gaattcctta gttgaaaaat aaagcgagaa atgcggtagc catgaaggga
     8581 atacctgtaa ttaacgcgcc atttcccggc gcatatacaa agatttctgt tttctgaata
     8641 tccactcatg ttcatccgga attaaatcac tatgttctgc gactgactta tgcataatgt
     8701 tttctctgtc tttcctgata cgcctgacat tcaacgcaca acgtcacacc gggaaatatt
     8761 ttccgccggg cttccggaat aggatttccg catgcttcac agagataaac aggaatacca
     8821 tgagcattta ttttctggcg tatccggttt attcctgtca tggtcagttg ttctgtcact
     8881 gaatatgctt catcggcttc atcactcacg gaatattcct cccgacatgt tctgacacgc
     8941 taccgcccat cccttttcac gcacagggca ctcgttttac aggcagattt tgaatacaga
     9001 aagcccgccg gcattgccgg ctgatataca acttcagggc cagttaatta atacgtggtt
     9061 ttccccacgc agacggttta ataacaaaaa acacgctgga tggctgatga taaacatcct
     9121 ggttatcaat ataaggcgct atccataatg ctgccgtctg ctcacccgtt cttagcggac
     9181 gaggaggtgt taccggcgta actgaactga ccggaacaac cgttttcact tcccgggcag
     9241 cggtaaacag cgggcgagga gccagcagtt tctgctccgg atgtgctgtc accgcaggtc
     9301 tgctgccgga cggagtggtc gcggtgacgg tctgcaccgg cattgtccgg aagttccctt
     9361 cagccaggcg cggcagtgat gccgcaaccg gctttgcttc tgaggactgc tccagttttt
     9421 tggccttctc attggcctgc tccatcgtca tacaggtatc ggatgtggtg gcgttacact
     9481 caaattccgt actggttccg gcacagccgg aaagtaataa ggtccccagc agaggaataa
     9541 agaaagaaat ctgtttcatt gtccctcacc tttctcatcg cagttcacaa aatccttctt
     9601 caaatccctg accgtacagg tttttacaat gtcaggattt tcaaaaaccg tactccgtga
     9661 tgatttataa acatatgcca caatgcgttt cccacccgca tcaggttctg gtgcactgac
     9721 ttcacataac ggaggtgtta ttccggactc atacagcacc ggataattta tttttttgac
     9781 gctgccatca gaaaccagct tattcatcga agggttcctt atatttctga attcttcatc
     9841 acaccgacag cagtcatatt aaaattgtcc ggcaatgact cagaaccgaa tttctccggt
     9901 gcaggaagta cgattctttt ggcgcagtta ctgagaacag catgcataaa atcatctgac
     9961 acggttttac ccggcacaga gagggctgta ataacattaa tattacgcat gttcattttt
    10021 atctccgttg tttctgtttg ttctgtcatc aggaagataa acagggcatt tcctgatttt
    10081 tcccggcagg gaagcgtctt ccgtataaac ttccttcaga tgttcatctg cggttaccac
    10141 ccggatacat ttcatttttc cgggtcgcac cggatatttc ccggtgatcg ctgccagcga
    10201 gacatacatc agtgccagtg ttgcccataa tgcggtgacc agttgcatca gtgaccggtc
    10261 agtctccctg acataatctg cagcatcagc cgcgcggtaa tggcagatat cctgtatttc
    10321 tgatctgcct gaagaatcag aggaagaaaa ccagcattca ctgaccggga actgtccggc
    10381 aggagctgtt tgtcttacga agttaatctc cttaaccagt aatttccccg gcgtggtgtt
    10441 gtccttccat aaaacaaaca gagccatcag ggccattgtg gccagtggca gaatgacggt
    10501 gtacttcaga caccagaaca gtcctcgcag aacccgggcg acaacatacc gggcggcatg
    10561 gcacgcctga cgggacgaca tattatttgc catcgttgcc tcctccctgg ctgccggaca
    10621 taaactgctg cggattcagg ttgcccagct gcttcagcat ctggtcagta ttaaagccgt
    10681 taagatgact ttcagccgat ggcggaacag gaaccggact ttccggattg tcttcttccg
    10741 ctctgttctg cgtgcgttcc agcgccatct cttccacggt tttcagctgg aagccgtcct
    10801 ggaacaccac ggtcacttcg ttgcccgcac caatcggtat caccgggtga tactgttcgg
    10861 cacgtttgat gtagtagtca ctgagcgtct gtgcggcttt cgatgcgccg ccaccgatac
    10921 ccattttcag gacatcacca gccccgtaag cggctgtggc acccagcccg acagccggct
    10981 gggaggcgcg ctccattccc tgaccgatac cgtcaacaaa tcccgcgccc catgcccagc
    11041 cgaggatttt gccgttacgc atcaccactt cgcccttgat accgttttta ccccggaagc
    11101 tgacatgccc cttaatcggc atatcaatag ttttgccgtc cttcaggcaa ctgatattgc
    11161 gggtgcgtac aatggcacgc tcactggaca catcccccca ggcttccaga cccacaaaac
    11221 atcccgttgc gtcatacgtc ttgctgttcg gcatttccac caggccggtg atacgcagct
    11281 gcatcggcac cgtggattca ttaccggtga cagaggcatt ggcatccgcc ccttcaatca
    11341 gcatggcttt cgcaaaactt cctgacggaa tgtacggcag tgatggtccc tgttttccct
    11401 cattacgggt aaacacctta cgctgtatcc ggttaggcac cggcacagac tggtacgtca
    11461 cctgtggtgg tggcgtgaca ccattcccgg gataaaacgc cgtcggcggt ggaacagcaa
    11521 cgctgccctg cggcgggaag gatacggggg tgtttcctgg ctgtggttcg ccttccgggc
    11581 cgggcggtga ggcaggcatc tgcagtacag gctcaccggt cgccgtgacg ggattagcac
    11641 ccagggcttt tacctgctct gccagggcgg cattgtcctg tcccaacttt tcaatacgac
    11701 gctggtcatc accgcgctgt ttgttcagaa catccagctc acgacggatt tcctcatact
    11761 gcttctgcat ctgcgctgcc gtcacctgca tctcggtggt ggcatgctga cgcaccttgt
    11821 catcaaaggt cgtatccacc acaccggtca tatccggcac aggctcctgt tcagccacgg
    11881 tttcaccgtt accggacatg tccacatcag acagatacag tgccccacca atcgcggagg
    11941 ctgtaccgac aaccacaatc cccagccaca ggtactgctt gcgtttcaca atggtattga
    12001 tactggccat cagttgccct ccccgtcacg gatgacatac acatccatgc gcgcacctgc
    12061 cagtaactgg ctggcaggct ggctgaacat cacggcacgg gttcccggct gccagaagtc
    12121 actttcccgg atattcaggg cagacagcgt tttattttcc acggcaaagc ggaccatctt
    12181 caggtgattc cccgtccata cggcatctgc cactgaagac agccccgccg gggtctgcag
    12241 ggtttccttt gtcaccggga cctgatacca gcctgcgggt aattttccgc cacggacgac
    12301 ctggctgatg gtcacaagca gggattcgta aggcgtggac gtttcccacg caccggcttc
    12361 ttctccggtt ccgcgcaggt cactgaccag ctgaatggta cgccccgcgc cttcacgggg
    12421 aacggcctga atggaaagat tcagaccacg ttctgtttcc agaatgaacg taaagggctt
    12481 tttgttgacg gtggccacca ccacaccgcc ggaggcggtc tgctcattat tggtcagggc
    12541 accatccaga ctgttcacgg cgataatccg gtcaccggga acggtaaaca gattcgggct
    12601 ggtattgctg atggcaatac tggcctggcc accgttcacc attggcacca cggtgggggc
    12661 cagcgtaccg actccggcca tcacgctgca ggaaaaaaac aggccgccta atattattgc
    12721 tgtattattt tttctcatcg tctgtttccc cgaaattatc cagccaggtc accccgtttt
    12781 cccgcttcag aataaggatg taatgtttga tatcagtgaa aggttttgaa tcaccaatcc
    12841 aggttttaag cacaccacga atttccacac ggccatactg aggccagaca cgaacactgg
    12901 tctggaaaaa ggctgagttc acgttatcgt ttttaatacg cttcgcttct tcagccagaa
    12961 taactttcat ctggttctgt gcgcccgggc ggatatattg cagaagcgcc tgatgtgagg
    13021 catcggcggt ttctgatgaa acattcagac ggagggcaat aaatgacagc gccatctgct
    13081 gtaaataaga ggcgtcggca ctgttctgtg acacggcaaa gggggcatta aatgccattg
    13141 gcgtcaccgc agtgcgttgc tcattctgaa gacggtagtt attcaccccc tgaatgacgt
    13201 taacagagag gctgagaaca ataagcactg acataaatat aaaggcgatg gccattacac
    13261 gactggtact taaacgggca ccgtgttcca tataacaatc ctggtatcag ttctatttaa
    13321 tccactgccg gaaacacgaa ttgggaacat tatgaaaaat accgcgcagc agggctgttg
    13381 gcatatacca gtaaatcagg tcacgtaacc aggaactgcc ccgccctttt ttcagttttt
    13441 taatcccgaa ataaaccaga accgctgcac caataccgaa cagatatttc gatgttgtga
    13501 taccccagcc aatacagatt gctgcgggga tcagttcatc cagtggcagg ccaaaccagc
    13561 ggctctggtt ggtcagtgtt tccgggaaac gatatttctt aagtttattc tcgtctcccg
    13621 acatcgtttt atttcctgtc agaggccaac gacggccata cccacagcaa taaatacaga
    13681 gatgatggca aaaccggcca ggaacttgac gtttttggtc atcatgtaca tgaccgcacc
    13741 gaccagaact tcagccagaa caacccattt aacaacactg gagtccttac cgaaggtcgc
    13801 cttaaccgtg gtgttaccac ttgccatcag gtcctgacca ctgctgccgg cggccatcgc
    13861 cagctgcggg aagaacatca tcagaacagc agccgggatc acagcgcgag ccaggcgaag
    13921 catattcaga cgagtgaatt tagaaaaaaa cgacttcttt ttgacgggcg cagaaacacc
    13981 ctgaacactt aaaacggcgt ccatatgatt cctctgttta atattgatag tgaaaaataa
    14041 aactctttat atatcgtcta tgctttcagc
//
LOCUS       DAPGWD010000108        13815 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPGWD010000108 DAPGWD010000000
VERSION     DAPGWD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.109"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1064
                     /gene="ugd"
                     /locus_tag="RHX88_003134"
     CDS             <1..1064
                     /gene="ugd"
                     /locus_tag="RHX88_003134"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDV2231796.1"
                     /translation="AMLNDRISPIVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVII
                     ATPTDYDPKTNYFNTSSVESVIKDVVEINPYAVMIIKSTVPVGFTAAMHKKYRTENII
                     FSPEFLREGKALYDNLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEA
                     IKLFANTYLAMRVAYFNELDSYAESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYC
                     LPKDTKQLLANYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSD
                     NFRASSIQGIMKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMA
                     EELKDVADKVYTRDLFGSD"
     gene            1210..2187
                     /gene="wzzB"
                     /locus_tag="RHX88_003135"
     CDS             1210..2187
                     /gene="wzzB"
                     /locus_tag="RHX88_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDV2231797.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(2282..2893)
                     /locus_tag="RHX88_003136"
     CDS             complement(2282..2893)
                     /locus_tag="RHX88_003136"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDV2231798.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQNLDLTTVIENLRKRHQ"
     gene            complement(2887..3663)
                     /gene="hisF"
                     /locus_tag="RHX88_003137"
     CDS             complement(2887..3663)
                     /gene="hisF"
                     /locus_tag="RHX88_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDV2231799.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            complement(3645..4382)
                     /gene="hisA"
                     /locus_tag="RHX88_003138"
     CDS             complement(3645..4382)
                     /gene="hisA"
                     /locus_tag="RHX88_003138"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDV2231800.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAVLLEAGVARVVVG
                     STAVKSPEIVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(4382..4972)
                     /gene="hisH"
                     /locus_tag="RHX88_003139"
     CDS             complement(4382..4972)
                     /gene="hisH"
                     /locus_tag="RHX88_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDV2231801.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            complement(4972..6039)
                     /gene="hisB"
                     /locus_tag="RHX88_003140"
     CDS             complement(4972..6039)
                     /gene="hisB"
                     /locus_tag="RHX88_003140"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDV2231802.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            complement(6039..7109)
                     /gene="hisC"
                     /locus_tag="RHX88_003141"
     CDS             complement(6039..7109)
                     /gene="hisC"
                     /locus_tag="RHX88_003141"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDV2231803.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQLSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            complement(7106..8410)
                     /gene="hisD"
                     /locus_tag="RHX88_003142"
     CDS             complement(7106..8410)
                     /gene="hisD"
                     /locus_tag="RHX88_003142"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDV2231804.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLASAERLTAHKNAVTLRVNALKEQA"
     gene            complement(8416..9315)
                     /gene="hisG"
                     /locus_tag="RHX88_003143"
     CDS             complement(8416..9315)
                     /gene="hisG"
                     /locus_tag="RHX88_003143"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDV2231805.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     misc_feature    complement(9362..9485)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(9461..9511)
                     /gene="hisL"
                     /locus_tag="RHX88_003144"
     CDS             complement(9461..9511)
                     /gene="hisL"
                     /locus_tag="RHX88_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDV2231806.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            9794..10045
                     /gene="yefM"
                     /locus_tag="RHX88_003145"
     CDS             9794..10045
                     /gene="yefM"
                     /locus_tag="RHX88_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDV2231807.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            10042..10296
                     /gene="yoeB"
                     /locus_tag="RHX88_003146"
     CDS             10042..10296
                     /gene="yoeB"
                     /locus_tag="RHX88_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDV2231808.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            10379..11203
                     /locus_tag="RHX88_003147"
     CDS             10379..11203
                     /locus_tag="RHX88_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2231809.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKSGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            11249..12178
                     /locus_tag="RHX88_003148"
     CDS             11249..12178
                     /locus_tag="RHX88_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2231810.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAMEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            12393..12455
                     /gene="yoeI"
                     /locus_tag="RHX88_003149"
     CDS             12393..12455
                     /gene="yoeI"
                     /locus_tag="RHX88_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDV2231811.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            12445..13803
                     /gene="plaP"
                     /locus_tag="RHX88_003150"
     CDS             12445..13803
                     /gene="plaP"
                     /locus_tag="RHX88_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDV2231812.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT         3239 a         3520 c         3593 g         3463 t
ORIGIN      
        1 ttgctatgct gaatgatcgg atatctccta ttgttgataa ggaaattcag cagtttttgc
       61 agtcagataa aatacacttt aatgccacat tagataaaaa tgaagcctac cgggatgctg
      121 attatgtcat catcgccact ccaaccgact atgatcctaa aactaattat ttcaatacat
      181 ccagtgtaga atcagtaatt aaagacgtag ttgagataaa cccttatgcg gttatgatca
      241 tcaaatcaac ggttcccgtt ggttttaccg cagcgatgca taagaaatat cgtactgaaa
      301 atattatatt ctccccggaa tttctccgtg agggtaaagc cctttacgat aatctccatc
      361 cttcacgtat tgtcatcggt gagcgttcag aacgcgcaga acgttttgct gcgctgttac
      421 aggaaggcgc gattaagcaa aatatcccga ccctgtttac cgactccact gaagcagaag
      481 cgattaaact ttttgcaaac acctacctgg cgatgcgcgt ggcgtacttt aacgaactgg
      541 atagctacgc agaaagttta ggtctgaatt cccgtcaaat aatcgaaggc gtttgtctcg
      601 atccgcgtat tggcaaccat tacaacaatc cgtcgtttgg ttatggtggt tattgtctgc
      661 cgaaagatac caagcagtta ctggcgaact accagtctgt gccgaataac ctgatctcgg
      721 caattgtcga tgctaaccgc acgcgtaaag attttattgc cgatgccatt ttgtcacgca
      781 agccgcaagt ggtaggtatt tatcgtctga ttatgaagag cggttcagat aacttccgtg
      841 cgtcttctat tcaggggatt atgaagcgta tcaaggcgaa aggtgttgaa gtaatcatct
      901 acgagccagt gatgaaagaa gactcattct tcaactctcg cctggaacgt gatctcgcca
      961 ccttcaaaca acaagccgac gtcattatct ctaaccgaat ggcagaagag cttaaagatg
     1021 tggcagataa ggtatacacc cgcgatctct ttggcagcga ctaacatctt gttatcaggg
     1081 ctatttacgc cctgattgtc ttttgttacc tccgcaataa ttcattattt ttatcgctta
     1141 tcctatagca ttcacgggga ttatcgctaa actatgcgga cttggaaatt tccgtcagtt
     1201 agggtaatga tgagagtaga aaataataat gtttctgggc aaaaccatga cccggaacag
     1261 attgatttga ttgatttact agtgcagttg tggcgtggca agatgacaat catcatttcc
     1321 gtcattgtgg ctattgccct ggctattgga tatttggcag tagcgaagga gaaatggacg
     1381 tcaacagcaa ttatcactca gcccgacgtg gggcaaattg ctggctataa caatgccatg
     1441 aatgttatct atggtcaggc tgcgccgaaa gtatcggatt tacaggaaac gttgattggt
     1501 cgcttcagtt ctgccttctc tgcattagca gaaacgctgg ataatcagga agagccagaa
     1561 aagcttacca tcgaaccttc tgttaagaac cagcaattac cattgactgt ttcttatgtt
     1621 gggcaaactg cagagggcgc acaaatgaag ttggcccaat acattcagca agttgacgat
     1681 aaagtgaatc aagagctaga aaaggatctc aaggacaaca ttgctctggg acggaaaaac
     1741 ttgcaggact ctttaagaac gcaggaagtg gttgcgcagg agcagaaaga tctgcgtatc
     1801 cgtcagattc aggaagcgtt gcagtatgcg aatcaggcgc aggtgacaaa gccacaggtt
     1861 cagcagactg aagatgtgac gcaagatacg ttgttccttt tggggagcga agcgctggag
     1921 tcgatgatta agcatgaggc gacccgtccg ttggtgttct catcaaatta ctatcagact
     1981 cgtcaaaacc tgctggatat cgaaagctta aaagttgatg atcttgatat tcatgcttac
     2041 cgctatgtga tgaaaccgac gttacctatt cgtcgcgata gcccgaaaaa ggcaattacc
     2101 ttgattctgg cggtgctgct gggcggcatg gttggcgcgg ggattgtgtt ggggcgtaac
     2161 gctctgcgta attacaacgc gaagtaatct tttcggtttt aaagaaaatg ggcagggtgg
     2221 tgacaccttg cccgtttttt gccggatgcg acaacaatat cgcatccgct taccgcgcaa
     2281 ctcactgatg ccgtttacgc aggttctcaa ttaccgtcgt taaatccaga ttctgatcct
     2341 gcaacaacac cagcaggtga tacatcaaat cagatgcctc gttggtcagt tcaaagcggt
     2401 catgtaccgt tgctgccagt gcggtttcca ccccttcttc gcccactttc tgcgcaatac
     2461 gtttggtgcc gctggcatac agtttggctg tgtaagaggt ttccgggtcg gcagatttgc
     2521 gttcggcgag caattgttcg agttgataca ggaacagcca ctggtgagcg gtgtcgccga
     2581 agcagctgct ggtgcctttg tggcaggttg gcccgatggg attcgccagc accagtaacg
     2641 tgtcgttgtc gcagtccggc gcaatactca ccacattgag gaaattgccc gacgtttcgc
     2701 ctttggtcca cagccgttgt ttagtgcgcg agaagaaggt gactttgccg ctttcgatgg
     2761 ttttgtctaa ggcttccggg ttcatatagc ccagcattaa cacttcaccg gataccgcgt
     2821 gttgcacaat caccggcatc agtccgtcgg ttttttccca gtccagttcg cgacgttgtt
     2881 gttctgttaa catatcctga tctccacgcc ctgtgttgcc aggtacgctt ttaattcacc
     2941 aatattgatt atttgtttgt ggaatacgga agctgccagc gcgccgtcaa cgtcggcatc
     3001 gcggaaggct tcgaggaagt gttccatggt ccccgcgcca ccggaggcaa tcagtgggac
     3061 gtggcaaact tcacgcactt ttttcagttg ttcgaggtcg taaccgttac gcacgccgtc
     3121 ctgattcatc atgttgagga cgatttctcc ggcaccgcgt ttttgcactt cctctaccca
     3181 gtcgagcgtt tcccactgag tgacgcgggt gcggctttca tcgccggtat attgattcac
     3241 atgatatttc ccggtttcgc cgtcgtacca ggtatcaata ccgaccacaa tacactgcac
     3301 gccaaaacga tccgccaggc gagtaattaa cgtcgggtcc gccagcgcag gagagttgat
     3361 ggaaatttta tccgcgccaa aggaaagaat tttcgcggca tcttccagag acttaatccc
     3421 acccgccaca cagaacggaa tgtcgatgac ttccgccacg cgagataccc agcttttatc
     3481 taccacacgg ccatcgctgg aagcggtgat atcgtagaac accagttcgt ctgcaccttc
     3541 ttcagcgtag cgttttgcca gcggcacgat atcgccaatg atttcatggt tgcgaaactg
     3601 tacgcctttc accacctgac catcacgaac gtcgagacac ggaattatgc gttttgccag
     3661 catgcgatgg cctccttcac ggtgaattta ccttccagta atgcacgacc aactattacg
     3721 ccgcgcacgc cagtaccacg cagggcagca acatcgttaa tgtcgccaat accgccggag
     3781 gactgaaatg ccacctgcgg gtatctggcg cacacttctt catataaaga gacgttagag
     3841 cctgccagcg tgccgtcgcg cgagatatcg gtacacagca catgtttcag gccgacgggc
     3901 agataggttt ccaccagttg ttccagtgaa acgcccgagt tctcttgcca gccgctgacc
     3961 gccacctgct tgttgccttg ctcgtcaata cggacatcca gcgccagcac taaggcatcg
     4021 gcaccgaagc gttcaaacca gcctttcaca atttctggtg attttaccgc ggtggagccg
     4081 accaccacgc gcgcaacgcc tgcttcaagt aacaccgcca catcttcttc gctacgcacg
     4141 ccaccgccaa cctgcaccgg aacgttaacg cccgcgacca gggttttaat cagcgggatt
     4201 tgacgttttg ccggatcttt tgccccggtc agatccacca gatgcaacac ttcggcacct
     4261 tgcgcagcgt aatcctgtaa gcgtggcagc gggtcgttac cgtaatcgcg ttgtttgccg
     4321 taatcgccct gatggagacg caccacagtg ccgtcaatta aatctaatgc cggaataatc
     4381 atcacatctc caggaagttt ttcagcaact tagcgccagc ggcaccagaa cgctccgggt
     4441 ggaactgcac gccgtagaag ttatcttttt gtactgccgc ggtgaacggt tcgccgtaat
     4501 tacactgggc gatggtccac ggattgactg gcattgcgta gctgtgaaca aagtaaaagt
     4561 acgcgccgtc ttcaatcccc tgaaacaggc ggttgcctgc ctgcgggtaa acgcggttcc
     4621 agcccatatg tggcagtggc agaccaaagt cggtcatttt cggcacgtct tcgtcgatga
     4681 tgcccagcaa gtcgacgccg ttgctctctt cgctgcgccg ccccagcagt tgcatcccta
     4741 agcagatgcc cagcaccggt tgggtacagg ctttgatgag atcaaacagc tcgcgctcac
     4801 gcacctgatc catcgctgct tgtgcggtgc caacgccggg taaaaatagt ttatcggcca
     4861 gcaacacgac gtccgggtca cggctgactt tgggttcata accgtggcgc gcaatggcag
     4921 acttcaccga gttcagattg gcgcagccag tatcaaggat caccacgttc attacagcac
     4981 tcctttcgac gagggcaggg tgtcgccttc cacgcggatg gcctggcgca gggtgcgacc
     5041 aaaggctttg aacagactct ctacacggtg atgatcgttt ttacctttgg ttttcaggtg
     5101 tagcgtcacg cccatggtgt atgagagcga acggaagaag tgctcgatca tttcggtgct
     5161 gagatcgccc acgcgctggt aggtaaactc ggctttatat tccaggtgcg ggcgaccaga
     5221 gatatccagc gcgcagcggg caaggcattc gtccatcggt agcacaaaac caaagcggca
     5281 aataccgcgt ttgtctccga gggcgatttt tagcgcttcg cccagcgcca ggccggtatc
     5341 ttcgacggtg tggtgatcgt cgatatagag gtcgcctttg acgttgattt ccatgcggaa
     5401 accgccgtgg gtggcgatct gatccagcat gtgatcaaag aagccaacac cggtgttaat
     5461 cttgctgcca ccttcgcgat ccagccacac ctggacgtca atttgcgtct ctttggtgtt
     5521 gcgcactaca tgggcgtaac ggtctcgttt agtgagttgc tcgccaatca tcggccagtt
     5581 caggatttcg cggtcgtagc gtaaaccatt aatgcccatg ttttccgcca gttgaatgtc
     5641 ggtcgcgcga tcgccaatca cataactgtt ggcacggtcc atcgcttgct cagccagata
     5701 acgctccacc agttttactt tcggcttacg gcagtcgcac tcatcggcgg gcaggtgagg
     5761 gcaaatcagc acttcatcaa actgcacgcc ctgtgaggtg aagatctgca tcatcaggtt
     5821 gtgcgggcca tcgaaatccg cctgcgggaa actttgtgtt cccagaccat cctgattagt
     5881 gatcatcacc agcttgtagc ccgctttttg cagcttcagc agttccggga tcacgcccgg
     5941 ttcaaaggcg agtttgtcaa aacggtccac ctgaaaatca ctcggcggtt cgctaatcag
     6001 ggttccatcg cgatcgataa aaagatactt ctgactcatc aaacttgctc cgcacgtaag
     6061 gcgtcaatga cgcgctggct ttcttcacgg gttccgacgg taattcgcag gcagccgctt
     6121 aaagagagtt gtttattctg atcacgtaag ataatgccct gatcccacaa agatttaaac
     6181 actgcactgg aggctttaaa gcgcgccaga atgtagttgg tttcagagtc aaaaacctgc
     6241 tccacgcagg ggatctcttt caatgcggca atcagatatt cgcgttctgc aataatttgc
     6301 gccacccgct cacgcatggc gacgatcccc tgcgggctta acgcctgggc cgcaatgtcg
     6361 gcaaccggcg tcgagagcgg gtagggggcg atcactttca ttaacaggtt gatgacctct
     6421 tcgtttgcca gcgtaaatcc gcaacgaagc cccgccagag caaaagcttt cgacagtgtg
     6481 cgcaaaatag ccaggtgcgg atattccgcc agccagccag ccagcgatgc ctgtggacaa
     6541 aactctatat aggcttcatc ggcaaccaca atcgccttac cgcgcgttaa ctccagcagg
     6601 gtgcgaaaat cctgcgggtt gatcagttgc ccggtcgggt tgttggggct gcaaacatag
     6661 accactttta cgccgtccag cttgtcggaa atgccctgta agtccagttg ccagttgtcc
     6721 agcgtcggca ctgtgcggca ctcgacgcca atcgtttcgg cgctgacgct gtacatgccg
     6781 tacgttggcg ggcagtagag gatggcgtct ttacccggtt cgcaaaaagc gcgaatcagc
     6841 agttcaatac cttcgtccgc gccacggctg accagcacct gctccggttt tacgcctgca
     6901 tattgcgcgt aattttcaat caccgctttc ggctggcatt ccgggtagcg gttgagcgtt
     6961 tgctgagtaa gctgaaactc cacggcagtg gggtattcgt tggcgttcag ccagacatcg
     7021 ccgttaccgc ccagacgacg cgctgactga tacggcgtca ggttgcggac gttttcacgc
     7081 gctaaatcgg taatagtcac ggtgctcatg cttgctcctt aagggcgtta acacgcaaag
     7141 taacggcatt tttatgggcg gtcaggcgct cggcggaggc cagtgtttca atggtcgaag
     7201 ccagcgcgga aaagccttct ttcgagagtt cctgcacggt catgcgcttc tggaaatctg
     7261 ccagcccgag gctggaacag gtggcggtgt aaccgtaagt cggtagaaca tggttagtgc
     7321 cagaggcata gtcgcctgcc gattccggtg accagtcacc aagaaatacc gaaccggcac
     7381 tggtaatgcc atcgaccagt tcgcgggcgt tgcgggtctg aatgatcagg tgctccgggc
     7441 cgtactggtt ggagatctcc acgcactgtg ctaaatcgtt ggtcacgatt aggcggctgg
     7501 cgttcagtgc ctggcgggcg gtttcggcac gcggcagttc tgccaactgg cgttcgacgg
     7561 cctcggcaac gcgacgcgcc atatcagcag cgggcgtcag taaaatcacc tgtgagtccg
     7621 ggccgtgttc agcctgagaa agcaaatcag aagccacgaa atctggcgta gcgccgctgt
     7681 cagcaatcac cagcacttcc gacgggcctg cgggcatatc gatcgccgca ccgtccagac
     7741 gctggctcac ctgacgtttc gcttcggtga caaaggcgtt acccggcccg aagattttgt
     7801 ccactttcgg cacagattca gtaccaaacg ccagtgcggc aatggcctgt gcgccgccga
     7861 cgttaaacac gtcctgtaca ccgcacagct gcgccgcata aaggatctca tcggcaatcg
     7921 gcggcggtga gcacagcacc acttttttac agcccgcaat acgcgccgga gttgccagca
     7981 ttaataccgt tgagaagagc ggggcggagc cgccaggaat atacaaccca actgaagcga
     8041 ctgggcgcgt gacctgctgg caacgtacgc ctggctgagt ttcaacatct accggcggca
     8101 gtttttgcgc agtgtggaag gtttcaatat tttttactgc caccgccatc gcctgtttta
     8161 gctcgtcgct caggcgttcg ctagcggcgg caatctcctc agcagacacc ttcagcgcgt
     8221 taaccgtggt tttatcaaac ttcgcgctgt attcccgaag ggcgtcatca ccgcgtgctt
     8281 tcacgttatc gagaatatcg ttaacagtgc gggtaatgct ttcagaagcg gagatcgccg
     8341 ggcgcattaa cagctggcgt tgttgctccg cagtacagct attccagtca atgattgtgt
     8401 taaagctcat ggcgatcact ccatcatctt ctcaatcggc aggaccagga ttgaactggc
     8461 acccagcgct ttcagttttt ccatggtttc ccagaacagg gtttcgctgc tgaccatgtg
     8521 catcgctacg cgttgttggt cacccgccag cggcagaata gttgggcgtt cggcacctgg
     8581 cagcagggcg atgacttcat ccagacgttc ggtcggtgcg tgcatcatga tgtattttga
     8641 ttcgcgcgcc tggatcacac cctgaatacg ggtcagcagt ttgtcgatca gttgctgttt
     8701 ggattcttcc atttcgccat cgcgttgtat caggcaggct ttcgagcgat agataacttc
     8761 gacttcgcgc aggccgttag cttccagcgt ggcaccggtg gaaaccagat cgcaaatcgc
     8821 atccgccagt ccggcacgcg gggcgacttc aacagaaccg ttcagtaagc aggatttaaa
     8881 agagatacct ttctggtcga gataacgctt gagcaggtga ggataagagg tggcgatacg
     8941 tttaccgttt aaggagagcg ggccatccca ggcttcatca accggcgttg ccagtgaaag
     9001 tcggcagccg ccgaaatcca gacgacgcag ggtaaagtag cgtggatctt caccctgggc
     9061 gcggcggtta agcagctctt cttccagcac gttttcgccg ataatcccga ggtcaaccac
     9121 gccgtccatt accagaccgg gaatgtcgtc gtcacgcacg cgcagaatat caatcggcat
     9181 gttttctgcc atcgcgatca ggcgctgggt gtgaagattg attttaatgc cacagcgcgc
     9241 cagcaattcg cgtgagtcat cacttaaacg gccggatttc tgcatagcta tgcgtaaacg
     9301 agtgttgtct gtcattttct tattcctctt taaacctgtc tgaaccgtta tcaaccgcgc
     9361 aataaaaaag cccccggaag gtgatcttcc gggggctttc tcatgcgttc atgcaccact
     9421 ggaagatctg aatgtcttcc agcacacatc gcctgaaaga ctagtcagga tgatggtgat
     9481 gatggtggtg tttaaattga acgcgtgtca taaaagtctc tgtgaatgtt tattcaactg
     9541 atgtctttta acctaaacca ctttcacgtt agaaagcaag ggctttttta tatattttat
     9601 ggcgatggat ttgtttaatg aatgagcggg gaaaggaggg ggctaagagg actgtcctct
     9661 aagctgacgg caccaaaaat agcgaagcct ggattttcgt ctcactgaca ataccttgat
     9721 ctacaaacta attaataaat agttaattaa cgcccatcat tgtacaatga actgtacaaa
     9781 agaggagatt gacatgcgta caattagcta cagcgaagcg cgtcagaatt tgtcggcaac
     9841 aatgatgaaa gccgttgaag atcatgcccc gatccttatt actcgtcaga atggagaggc
     9901 ttgtgttctg atgtcactcg aagaatacaa ctcgctggaa gagacggctt atctactgcg
     9961 ttcccccgct aacgcccgga gattgatgga ctcaatcgat agcctgaaat caggcaaagg
    10021 aacagaaaag gacattattg agtgaaacta atctggtctg aggaatcatg ggatgattat
    10081 ctgtactggc aggaaacaga taagcgaatt gttaaaaaga tcaatgaact tatcaaagat
    10141 acccgcagaa cgccatttga aggtaagggg aagccagaac ccctgaaaca taatttgtca
    10201 ggtttctggt cccgacgcat tacagaggag caccgtctgg tatacgcggt taccgacgat
    10261 tcactgctca ttgcagcatg tcgttatcat tattgaacag attcattcat agaatgaatt
    10321 gtcagttttg atacgctagc gtagccttat agatctaagg tgtatcagga gataacggat
    10381 gaaaaaggtc gcaattgtcg ggttagggtg gttaggcatg ccgctggcga tgtcactttc
    10441 agcgcgaggc tggcaagtca ccgggagtaa aaccacacaa gatggtgtcg aagcggcccg
    10501 aatgagtggc attgatagct atctgcttcg catggagcct gagttagttt gcgattctga
    10561 cgatctggat gccctgatgg atgccgacgc gctggtcatt acgcttccgg cacgtcgtag
    10621 cggccccggc gatgagttct atttacaagc ggtacaagag ttagtggata gcgcgctggc
    10681 ccatcgtatt ccccgaatta tttttaccag ctcaacgtct gtctatggcg acgcgcaagg
    10741 cacggtgaaa gaaaccaccc cgcgtaatcc ggtaaccaac agtggacgag tattagaaga
    10801 actcgaagac tggctgcaca atttacccgg tacttcggtc gatattctgc gtcttgcggg
    10861 gctggtcgga ccgggacgtc atcccggacg cttctttgcc ggaaaaaccg cgcctgatgg
    10921 tgaacatggt gttaatttag tccatttaga agatgttatt ggcgctatca ctctgttgtt
    10981 acaggcacct aaaagcggac acatctataa tatatgtgcg ccagctcacc ctgcgcgtaa
    11041 tgttttctat ccgcagatgg cccgtttact ggggctggaa cctccgcagt tcagaaatag
    11101 tctggacagc ggcaaaggta agattattga tggcagtcgg atttgtaatg aactggggtt
    11161 tgaataccag tatcccgatc cgctggtaat gccgctggag taaacgatca cgccagcagg
    11221 acgccgcata cactgcaagg gggcgtgtat gaaaccactg ctggatgtgt tgatgatcct
    11281 cgatgcctta gaaaaagaag gcagttttgc ggcggcgtcg gccaaactct acaagacgcc
    11341 gtccgctctg agttacaccg ttcacaagct ggaaagcgac cttaatattc aattgcttga
    11401 tcgtagcgga caccgcgcta aattcacccg caccggaaaa atgttattag agaaagggcg
    11461 ggaagttctg cataccgtgc gggaactgga aaagcaggcg atcaaactgc atgaaggctg
    11521 ggaaaacgag ctggtgattg gcgtggacga cacgtttcct ttttctctgc ttgcgccact
    11581 gattgaagct ttttatcaac atcatagcgt gacgcgcctg aagtttatca atggcgtact
    11641 cggtggttcc tgggatgccc tgactcaggg gcgagcggat attattgttg gcgcgatgca
    11701 tgagccacca tcttccagtg agtttggttt ctcgcggttg ggcgatctgg aacaagtctt
    11761 tgcggtcgcg cctcatcatc cgttagccat ggaagaagag ccgttaaatc gtcgtattat
    11821 taagcgatat cgggcgattg tggtggggga taccgcgcag gcaggcgctt ctacggcctc
    11881 acaacttctc gacgaacaag aagccattac cgttttcgat tttaaaacca agctggagct
    11941 gcaaattagc ggcctcggct gtggctattt accccgttat ctggcgcaac gttttctcga
    12001 tagtggcgcg ttaatcgaga agaaagtggt cgcccaaact ctctttgaac ccgtctggat
    12061 tggctggaac gaacagaccg cagggcttgc cagcggctgg tggcgggatg aaattttagc
    12121 aaatagtgcg atcgccggtg tttatgcaaa atctgatgac ggaaaatcag ccatttaaag
    12181 aaaaattatt atgacaagcc tctcattctc ttgtcatttc ccccccattt aggcacaatg
    12241 cgccgctgtc aaaaaatgac taaaaaccga cgtttcatca gcgtcggtta ttttttgctt
    12301 caaaccaatc attcatacca agaggccggg cttcgtaccg gatagatatt tactaaaaat
    12361 cgacagttgt tgtcgctgag gaatccaaaa aaatggggca attttttgct tacgcgacgg
    12421 ttatcaccgt aaaggagaat gaccatgtcg cataacgtta ctccaaacac ctctcgcgtg
    12481 gaattgcgta aaacgcttac gttagttccg gttgtaatga tgggtcttgc ctatatgcag
    12541 ccgatgacgc tgtttgatac ttttggtatc gtttcaggcc tcacggatgg tcatgtgccg
    12601 acagcctatg cgttcgcatt gattgcgatc ctgtttacgg ctctgagcta cgggaagctg
    12661 gttcgccgct atccttctgc tggctctgca tacacttacg cccagaaatc cattagcccg
    12721 actgtcggct ttatggtggg ttggtcttct ctgctcgact atctgttcgc gccgatgatc
    12781 aacattctgc tggcgaaaat ctattttgaa gctctggtgc cttccatccc atcgtggatg
    12841 tttgtggtgg cgttggtggc cttcatgacc gcctttaacc tgcgtagtct gaaatccgta
    12901 gcgaacttca acactgtaat cgtcgtgttg caggtggtgc tgatcgcggt gattctgggc
    12961 atggttgttt atggcgtatt tgaaggtgaa ggcgctggta cgctggcgag cactcgtcca
    13021 ttctggtctg gcgatgcgca tgttatcccg atgattaccg gggcgacaat cctgtgcttc
    13081 tcctttaccg gctttgacgg catcagcaac ctgtcggaag aaaccaaaga tgcagagcgc
    13141 gtgatcccgc gtgcgatttt cctgaccgcg ctgattggcg gcatgatctt catctttgca
    13201 acttacttcc tgcagctgta cttcccggat atctctcgct ttaaagatcc ggatgcgtca
    13261 cagcctgaaa tcatgctgta tgttgcaggt aaagcattcc aggttggtgc gctgatcttc
    13321 tccaccatta ccgtactggc gtccggtatg gcggcgcacg caggcgtagc gcgtctgatg
    13381 tacgtaatgg gtcgtgacgg cgtgttcccg aaaagcttct tcggttatgt acacccgaaa
    13441 tggcgtactc cggcgatgaa catcatcctg gttggcgcga ttgccttgct ggcaatcaac
    13501 ttcgacctgg taatggctac agcgctgatc aactttggtg cgttggtggc gttcaccttc
    13561 gttaacctgt cggtcatctc gcagttctgg atccgtgaga agcgtaacaa gacgctgaaa
    13621 gatcacttcc agtatctgtt cctgccgatg tgtggtgcgc tgaccgttgg tgcgctgtgg
    13681 gttaacctgg aagaaagctc aatggttctg ggtctgatct gggcggctat cggtctgatt
    13741 tacctggctt gcgtcaccaa gagtttccgt aacccggttc cgcagtacga agacgtagcg
    13801 taatcatctt ttgta
//
LOCUS       DAPGWD010000109        13557 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPGWD010000109 DAPGWD010000000
VERSION     DAPGWD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.110"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            181..1011
                     /locus_tag="RHX88_003151"
     CDS             181..1011
                     /locus_tag="RHX88_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDV2231813.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1152..1925)
                     /gene="uxuR"
                     /locus_tag="RHX88_003152"
     CDS             complement(1152..1925)
                     /gene="uxuR"
                     /locus_tag="RHX88_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDV2231814.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2140..3600)
                     /locus_tag="RHX88_003153"
     CDS             complement(2140..3600)
                     /locus_tag="RHX88_003153"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDV2231815.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3681..4865)
                     /gene="uxuA"
                     /locus_tag="RHX88_003154"
     CDS             complement(3681..4865)
                     /gene="uxuA"
                     /locus_tag="RHX88_003154"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDV2231816.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5205..6548
                     /gene="gntP"
                     /locus_tag="RHX88_003155"
     CDS             5205..6548
                     /gene="gntP"
                     /locus_tag="RHX88_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDV2231817.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6792..7694)
                     /gene="fimH"
                     /locus_tag="RHX88_003156"
     CDS             complement(6792..7694)
                     /gene="fimH"
                     /locus_tag="RHX88_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDV2231818.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7714..8217)
                     /gene="fimG"
                     /locus_tag="RHX88_003157"
     CDS             complement(7714..8217)
                     /gene="fimG"
                     /locus_tag="RHX88_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDV2231819.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNNGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8230..8760)
                     /gene="fimF"
                     /locus_tag="RHX88_003158"
     CDS             complement(8230..8760)
                     /gene="fimF"
                     /locus_tag="RHX88_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDV2231820.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8770..11406)
                     /gene="fimD"
                     /locus_tag="RHX88_003159"
     CDS             complement(8770..11406)
                     /gene="fimD"
                     /locus_tag="RHX88_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDV2231821.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11473..12198)
                     /gene="fimC"
                     /locus_tag="RHX88_003160"
     CDS             complement(11473..12198)
                     /gene="fimC"
                     /locus_tag="RHX88_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV2231822.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12235..12774)
                     /locus_tag="RHX88_003161"
     CDS             complement(12235..12774)
                     /locus_tag="RHX88_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2231823.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12839..13387)
                     /gene="fimA"
                     /locus_tag="RHX88_003162"
     CDS             complement(12839..13387)
                     /gene="fimA"
                     /locus_tag="RHX88_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV2231824.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3288 a         3509 c         3274 g         3486 t
ORIGIN      
        1 ataatttcaa atgagcgttt tttgatactt tagagtgttt cctacccttt aatatcaccg
       61 tgtagagttg atatcactac aacagaaaca tgcatgtcat ttgtttactt ctctttttac
      121 tacagaaaaa tttatctatt tttctaaaca tcagtgaaat actcctaaaa ggtaactatt
      181 atgtctatgc cattaagcaa tgcccttcag agtcaaatta ttacagataa tcacttcctt
      241 catcacccaa aagtcgacaa tgagttaaca cgtaaatatg aacgagctcg cctggatacg
      301 gaaaatatct atcttctgcc tcttgctcgt ggcaataacc ataattacga tggaaaaagc
      361 gttgtcgaaa ttagaaaact tgatattagt aaagagtcct ggccatttaa ttatgtgaca
      421 gaagcatgca gagaatccga tggtattaca acaacaggac gtatgctcta tcgaaacttg
      481 aaaatcacct cagctttaga cgaaatatat ggtggtatat gtaaaaaggc gcatgctgct
      541 acagaattag cagaaggttt acggctaaat ttatttatga agagcccttt cgatcctgtt
      601 gaggactata cagttcatga aataacatta ggccctggtt gcaatgttcc aggttacgcc
      661 ggaaccacga taggatatat ttcaaccctt ccagtttctc aggcaaaaag atggaccaat
      721 gagcagccaa gaattgatat ctatattgat cagataataa ctgtctcagg ggtagcaaat
      781 tcttccggat tcgcccttgc cgcattactt aatgctaata ttggaatggg aaatgaccca
      841 ataattggta tagaagccta tcccggtacc gcggagatcc atgcaaaaat gggatataac
      901 gttatccccg gtgatgaaga tgcgccactc aaacgcatga ctctgcaacc gtcatctctt
      961 ccagaactgt ttgaattaaa aaatggtgaa tggaattata ttggtaaata gccactgaga
     1021 agaatgagta ctaatattca ggttatacag atatttaaac aataccctgc aatttacaaa
     1081 gtaatactca ttgcgacttc ttaatttaag aaagcgcaag gaacgtttac ccttgcgctt
     1141 attataataa gtcaggcgtc gactttatcc agcggccatg aatcaaacag atagccgtca
     1201 aaataaatat cgtcaacgtt cgagaattcc agcagacgtt gcttaacgtt ttccagatgc
     1261 tgccacattg ccagcttcgc cgctcgggca tcttttttga ttaacgcggc gaggatctgt
     1321 ttgtgatcgc ccaaccactc tttgcgatac aggctgtcat ccagatggct gtgcaactgg
     1381 atccacattg ggttgttttc ccgccactgc caggactgac ggaacagctc caccagcatg
     1441 ctgttatgcg ttgcttcggc aatagcgaga tggaactgca tgtcaccgct ttcgctgctg
     1501 cccggcgcac tggaagccag ttcacgctct tccagttgca atgcctgacg cattttgacg
     1561 atatcttcgc gggtagcctg cagggcggca aactcggcga tgttactttc cagtaactga
     1621 cgcgcctgta acagctcaaa aggaccggca tcgttgcaga cgttggcatc cggactgtct
     1681 gtgttctggc tgcctgagtt atcaagaaca tagataccgg caccccggcg tacttccacc
     1741 agccctttga tctccagcat gatcagcgct tcacgtacca ccgtccgcgt gacatcaagc
     1801 atttctgcaa tttcacgctc cggcggcagc cgttcgccag ggttgtacgg cgtctttatg
     1861 atcagatcgc ggatcatcgc cccgacttcc tggtaaggtc tttgcgcaga ggtggcagat
     1921 ttcatggtgt tgtgtccgtt aggtgcggtt aatcaaattt ttgccggatg cggcgtgaac
     1981 gccttatccg gcctacaacg ggcaatttta cggcccccgt taatctcaaa ctataccatt
     2041 agcactggaa gttggcacgt acaggtgggc aaaaaaacga aaactggtca accaaatatc
     2101 agtttgattg accagtaagt ctgtagggta atcagttagt tacagcgcag ccacacactc
     2161 gcgcgcaccg cgttcgcaca gctgctgata tgccgccgtc actgcgccaa caaagtcggc
     2221 attctgcggc agatcatcgg caaaaatacc gctcaggccc agcagcgctt tcacgcggtc
     2281 tgcgccctga tactgcgcgt tgatcttctg gaactccgcc agcatcgggt cgaccacgtc
     2341 aatggcatta ccctgctcat ccacgccctg ggtgtaacgc atccagccag ccacgcccag
     2401 cgccaggtga cgccagctgc cgccgttttg caggtgcaga cgcaccgggt ccagcagacg
     2461 ctgcggtaac ttctggctgc cgtccatcgc aatctgccag gtacggtgac gcagagacgg
     2521 gttgctgaaa cgctcgatca gcagcgtcgc ataggcgttc aggtctgtac cttccggcat
     2581 cgacagcgtt ggcgcttgtt cctgcatcat cagggcaaag gccgctttgc gataagccgg
     2641 gttagtcatg gtgtcggcaa tggtttcata gccgccgagg taaccgaggt acgccagaaa
     2701 agagtggctg ccgttcagca tacgcagctt catcatttcg aacggcacaa cgtctgcaac
     2761 gaactgtgcg cccactttat cccagtccgg gcgaccatta acgaagttat cttcaatcac
     2821 ccactgacgg aacggttcgc aggcaatggc gcacgggtcg taaacaccca gctggtcagc
     2881 aatttcctgt aaggtttctg gcgtcgccgc cggaacgatg cggtcaacca tggtgcacgg
     2941 gaaggtgaca ttttcttcaa tccatgccgc cagctgcggg tcacgcgcct gagccagccc
     3001 cagtaccgcg accttcgcca catgtccgtt ttcacgcacg ttatcgcagg acatcaccgt
     3061 aaacgctttc aacccttttt cacgacgcag acgcaaggct tcgacgatgt aaccaatcgc
     3121 ggacttcggc gcagtcgggt tttccagatc gtgcttgatc agcgggttat tgagatccag
     3181 ttgaccgctt gccgcatcag cgcagtagcc tttttccgtg accgttagag agacaatcgc
     3241 cgtttgcgga cgcgccatcg cgttgagaat accttcgcag ccatcgattt ccggatgcag
     3301 cgcttctttc atcgaaccga taattttcag ctcggtgctc tctgcgcctt tttccgctac
     3361 ggtgtacagc agttgctgtt ttttcaggtt ttcgatcagc acgcggtcgt tgcctggcat
     3421 caggttaact tcgcagatgc cccagtcgct gtcggtgctt tccagcagat gatgggtata
     3481 cagcgcctgg tgcgcgcggt gaaacgcccc gcaaccgaga tgcacaatgc gtgattccag
     3541 acgagaatga tcccatgacg ggcgggcaac cggcagattg ctgtcaacaa tagtagtcat
     3601 cgtaaactcc agaatccgtc ttcagagagt gacggcaagg aagaaggaac cggcacggcg
     3661 cgcagccatg ccggtggata ttaacggcta aagaaagcgc gctggatcgc cagttcgaca
     3721 ccgcgaactt cggccaggcc tttcagacga ccaattgcgg agtaacctgg gttggttttc
     3781 ttcttcaggt cgtccagcat ctgatgaccg tggtccggac gcatcgggat caggtcttct
     3841 ttgccttccg ctttacgacg gtgttcttct tcaacaatcg ctttcaccac ttcgtacata
     3901 tcaacgtcac cgttcaggtg cgccgcttcg tggaaggttt tcgggttatc ttcacgcatg
     3961 gtggagcgca gatgggtgaa gtaaatacgc ggaccgaact gcttgatcat atcaaccaga
     4021 tcgttgtcag cacgcacgcc gtaggaaccg gtgcacatgg tgaaaccgtt cgccatgctg
     4081 tttacggtat caaccatcca ctgcatatct tcaatggtgg aaacaatgcg cggcaggccg
     4141 aggatcgggc gcggcggatc gtccgggtga accgccatac gcacgccaac ttcttcagca
     4201 actggaataa tcgctttcag gaagacggca aagttttcgc gcagcttcgc tttatcgata
     4261 tctttgtaca gctccaggtg cttacggaac tggtcgaggg tatacccttc ttccgcgccc
     4321 ggcagaccgg caatgatgtt acgcgtcaga cgcgctttgt cttcgtcgct catagtggcg
     4381 aagcgttcag cggcctgagc aatttcttct tcggtgtaat ccgcttccgc gcctggacgt
     4441 ttcaggatat gcatttcgaa tgcagcgaat tcgatctggt cgaagcgcag agctttggag
     4501 ccgtctggca gcacgtattc gaggtcagta cgggtccagt cgagcaccgg catgaagttg
     4561 tagcacacgg tgcgaatgcc gcactgcgcc aggttgcgca gagtctgctg atagttagcg
     4621 atccactgct catagttgcc agtgtgggtt ttgatatctt cgtgaattgg cacgctttct
     4681 acgacagacc ataccaggcc tgcgtcttca acgatcgcct tgcgtttgag gatctcttct
     4741 acggaccata cttcgccgtt cgggatatgg tgcagcgcgg taaccacgcc agttgcgccc
     4801 gcctgacgga catcagctaa agaaaccgga tcgtttgggc cgtaccagcg ccaggtctgt
     4861 tccatgattt ttcctctttc acatagtggg ttccacagca aagagacgcg gaaccctgga
     4921 gttgtttagg tatccggatg aacaaccaga ccgcagccgt ggcgttgcat cggatgagat
     4981 ggcgtataag ttctaccttt tggtaagaca tgtcaacccg aattctgaaa ttggttaacc
     5041 acatcacaag aatttcactc tcatggttgt tgctgcaaat tgcagcaaag ggtaatcgat
     5101 ggttattgat cagaggatgt gacattcatc gcaacaatgg ttgaccaatt tacataacat
     5161 gtcgcccaaa ataacaacgg ttcaaccagg taaggtaaaa cgttatgcat gtgcttaaca
     5221 ttctctgggt ggtattcggc attggcctga tgctggtact gaatttgaag ttcaaaatca
     5281 attcaatggt ggctttgttg gtggcggcgc tgtccgtcgg gatgctggcg ggcatggatt
     5341 tgatgtcgct gctgcacact atgaaagcgg gcttcggcaa cacgctgggg gaactggcta
     5401 tcatcgtggt gttcggtgcg gtcatcggta aattgatggt cgactccggc gcggctcacc
     5461 agatagcgca tacgctgctg gcgcgtctcg gtctgcgcta tgtacagctg tcggtgatta
     5521 tcatcggcct gatttttggt ctggcgatgt tctatgaagt ggcctttatc atgttagcgc
     5581 cgctggttat tgttattgcc gccgaagcta aaattccgtt cctgaaactg gcgatcccgg
     5641 cagtagcagc tgccactacc gcacattcac tgttcccacc gcagccgggt ccggtggcgc
     5701 tggtgaatgc ttatggcgcg gatatgggga tggtttatat ctatggcgta ctggtgacga
     5761 tcccaagtgt aatctgcgca ggtctgatcc tgccgaagtt cctcggcaat cttgagcgcc
     5821 caacgccatc attcctgaaa gcagatcaac cggtagatat gaataatctg ccctctttcg
     5881 gcgtttcgat tctggtgccg ctgatcccgg cgatcattat gatctctacc accatcgcca
     5941 atatctggct ggtaaaagat acccctgcct gggaagtggt taactttatc ggttcctcgc
     6001 cgattgcaat gtttattgcg atggtggttg cattcgtact ctttggcacc gcacgtggtc
     6061 atgacatgca gtgggtgatg aacgcttttg aaagcgcggt gaagagcatt gcaatggtga
     6121 ttctgatcat cggtgcgggt ggcgtgctga agcagaccat catcgacacc ggcattggcg
     6181 acaccatcgg catgttgatg tcccacggca atatctcgcc ctacatcatg gcatggctga
     6241 tcactgtgct aattcgtctg gcgacgggtc agggtgtcgt ttcggcgatg accgccgccg
     6301 ggattatcag tgctgcaatc cttgatccag caactggtca gctggttggc gtgaatccgg
     6361 cgctgctggt actggcgacg gctgcgggtt ccaacaccct cacccacatt aacgatgcat
     6421 ctttctggtt gttcaaaggt tactttgacc tgtcagtaaa agacacgttg aaaacctggg
     6481 gtctactgga gctggtcaac tccgtggttg ggcttattat tgtgctgatt attagcatgg
     6541 tagcgtaaaa ggacaccaga gcctgccaat ggcaggctca gactgatgac aaagtctaaa
     6601 aatgtgccca gacagtcccc tcgcccctcc ggggtgaggg ttagggtgag gggaaaagac
     6661 ccgcaaagtg cgctttgttt atgccagatg cgacgctgac gcgtcttatc tggcctacaa
     6721 agggctaacg tgcaggtttt tagcttcagg taatatttgc gtacctgcat tagcaatgcc
     6781 ctgtgatttc tttattgata aacaaaagtc acgccaataa tcgattgcac attccctgca
     6841 gtcacctgcc ctccggtacg agcgtaattt gccgttaatc ccagacttac cgccgaagtc
     6901 cctactgctc ctaacgatac cgtgttattc gctggaataa tcgtaccgtt gcgcgtcaac
     6961 tgtacgccga cgccctgcgc tggtgaaaac gaggcggtat tggtgaaaat cgagttgccc
     7021 gcatctgcgg ttgtgccgga gaggtaatat cccaggtttt ggcttttcgc acaataaacg
     7081 gtaagaggaa ttggcactga accagggtag tccggcagag taacggtgac atcacgagca
     7141 gaaacatcac agccgccagt aggcaccacc acatcattat tggcgtaaat attccacaca
     7201 aactggaaat catcgctgtt atagttgttg gtctgtcgca aaataagcac ggcaattaat
     7261 gagccagctt taatcgccac cccgcccgca ctgctcacag gcgtcaaata aagcgccacc
     7321 ggccacggct tatccgttct cgaattataa acaacgcgcg gcgtttcgct ggtggtcgga
     7381 aatggatagc tactgccact atattttacg gtcccggaaa aattagataa cacgccgcca
     7441 taagccgagc ctcgttgcag tgtgacatag tctgtaatgg tttccggata atcgttatgg
     7501 caaaagattt gcgtcgaaag atctacgacc aggttttgcc ccacattcac ggcaggcgca
     7561 aggtttacat aaacattagc gctgccaccg ccaataggga tagcggtacc attggcggtt
     7621 ttacaggcga atgaccaggc atttaccgac cagcccatca gcagtacagc aaacagggta
     7681 ataactcgtt tcattacaat catctcttcg ggttcagctg taggtatagg tgatgctaat
     7741 cactgcctga atggttccct gagtggctcc gccatttact gtcaatgctc tgacctgtaa
     7801 cgggaagtgc gctgattgtg aggaatcatc cacctgaact gttttggttg cgccattatt
     7861 caatgtgttg ccactgtcat cctgtagctc taactggatg ttttgcgcgg tcccctggtt
     7921 tttataatat ccggtactgt cggctgcccc gctgaagctg gcagtgaccc tcgacgttcc
     7981 caccggacaa ttagtcaact caagcgcaac atcatgccag gccgatgccg ccccggcaga
     8041 cataagactg aaagaataaa gatcgccgag atcaaccgtg gcattggtgg tggaaactgt
     8101 gcacggtttg gcgacgacct taccgttcac cgtgatggtg acatcggctg cctgtatcgt
     8161 cgcacttgcg agcgccaata ttgccgccaa taaataccca cgtttgcgcc atttcatgag
     8221 catctccagt tactgatatt caagagtgaa ggtagccgtg gcattgatat gccccgcagt
     8281 gactggcacc tgtgtcgcca ttagccgggc gtaaaaattc agcgtatttg gtttacccgg
     8341 cgtcagggtc gtccacgaaa tcgcggacga tggagcatta aggggtattt gattttgctg
     8401 ctcattcaga agctgtattc ccagtcccgc agccgctgac accgtatttt caagtgcaag
     8461 caggttggca ttgtggctat ctgcaacgcc agtaaaccca acctttacgg cagaaacggc
     8521 gttaccacag ggtgacagca aaatacgaaa tggaacgaca ggagtcgtcg cgccaatgtt
     8581 gttaaattgc ttcgccgcgt tttccatcag atcaacagta aaattggttg attcagcggc
     8641 cacactacag ccgttatctc tgacatagcc gcggatagta atcgtgctat ccgcagccaa
     8701 agcgtgactc accgccagcc acaaaaaagc gcacagaaga taaaaaggtt tgtttctcat
     8761 cacgccccct taacgacatt cagctgatag ctgggttaat aactgctgct gactctctgg
     8821 tggcagttga taattggcga cacagtgagc attttcctct tctccccatt tcacctgaac
     8881 ttttcccgct aaaggcattc cgctgaggta aacctgacca ttatccgcaa caatgccgct
     8941 actctggcta ctctctgatg tcaccatcgc cccaaacggc agcggcttat tattgtgggt
     9001 tagcgtcatg agcagtttta tcccaacgcg cgctttaaac tctgctcgca cgatcgcccc
     9061 acgagtggga acaacgttag cgaccgcgtt atctaaatcg acgttatcag ccagggtatt
     9121 ggtatccagc gccactctgt tttcccgata ttcagtggca taaggcagca cggcataacc
     9181 acgccagtcg gtacgcaccc ccgtctggtt ttcgactttt gcatcttttg cgccaggcgc
     9241 tttaacaagc accactgttt cgtttaacgg ctgccccagc gttacgccat tggcatgagc
     9301 caatacccca ccgctgactc cgtaatagag ctgcttaata tcatcgctat ggctgtaacc
     9361 gatattggca ttgccgtaac caccgcgata attcagcgtg gcgtagcctg tgcttccgct
     9421 attaccatcg cctcccccgg catagccggt ttgcacgcta tagctgaggt tgttgtcttc
     9481 cagcaacgta ccgtatacgc cagccagatt ggtcatccga ccgttgagat cgtgtgacat
     9541 gctgtagctg gcactggcat gtcgccactg agatttactg tcagaacgca gccagtggct
     9601 gaaaggaata ttgacgttaa gcgctaacat ctgatcccgt cctttttgcc aggcattttt
     9661 cgtcaggcta tagctgagcg tccagttgat atcttcgaat gcagtattta aaccagcctg
     9721 gaattgctca tcgacattac tcgttcccca ataagtttga tggctaccac tcaaatacag
     9781 tgttgatgtg cgcccgagtt gctgagtaac ggtgagctgt aatttcccgc gtttgttata
     9841 agcgaggttg taatagtcgg tgaatttcgg cttaacctga ataactccgt cctgtgtttc
     9901 gatgttgtag ccattcattc gactgtatgt tgtatcagcg aaattaaaat atccgctggt
     9961 cgaataacgg taacccacta actgaatatt cgtgcctgac tcattgagcg atttgttata
    10021 gagaaaacgc accgattgtc cgtcatgctg actgtcatcg ggaagtgtgg agttagcctg
    10081 cgtcatatcc acagacagag cgcccagtgc ccccatgttt ttcccgatac cgaaattaaa
    10141 agcacgataa cgatccgcca gttgcgttcc accatatatt gtccagccag ccggaaggcc
    10201 gtggagtaat gtactctgga aaaagcgggg tttttcctgc tgcgcatttc cactacggta
    10261 ttctcctgcc gtaatggaat aacgagtatg cccttcacgt tgcaaaagcg ggactgacga
    10321 atagggtacg gtaaaaatct gcgtgctgcc gtcagcctct ttgatcgtta cctgcaagtc
    10381 accactatta cctgcggcat agatatcgtt gatggtaaaa ggccccggtg gcaccgtact
    10441 attataaatg tcatacccat tttgtttaat agtgacctgt gcagtaccac gagcaatacc
    10501 gtggatcacc ggggcaaatc ctctttggct atcgggtaac atattgtcat ctgaggccaa
    10561 ttgtgcgccg cgaaagttaa taccatcgaa aatatcgccc tgagtataac catcacccag
    10621 cgtcagccgg gaacgtaacg gtattatgtc tcgctcaagc caggtattga tatgctgcca
    10681 tttatttttg ctacctgatg atctgtcgct actgttataa ctccaggtgg tattgtcgcg
    10741 taaacgccac gcaccaatat ttaacccact ctgtaggttt aaatatgcat aatggctgtt
    10801 acccccaatc cgattctgta cactatttcc gctgaaatta taattgagca atccggcatt
    10861 aataccggga tcccataact caggaggaat ataaccacgc gcgcgattac tcataaatgc
    10921 ctgagggatc gtcaggttga gtcgctgctg accaacatct aaatgcgcag tagcgtcatg
    10981 gatcattgag gttaatggca cgcaggcatc atccgccagc agattcatac cggagacaga
    11041 agccgtattc agccccatac tggcaagttg cgcgcgtgtc aggcagggaa caatcccttg
    11101 ttcactgtcg cccgtattaa atgtgacatc acgcgttgcc atataaccat tattcaaata
    11161 gatatcgaca cgatacgtcc ctggcggtaa ttcttgccca ttttcaaaac gcgataaatc
    11221 ggccacagcc tggggatcat ccgctaaaaa gcgcggatta aaatagagtt cggcagatga
    11281 caaaggtgcc tgtgcggcaa aagcacaggc gacaacgagc cggacaaaaa aaccagccaa
    11341 acgatgctta cgaatatgca agcattgtgt gtttcgctgg taaagtctta aattcagata
    11401 tgacattatt cctccggttt aaaatcaccc ggcagtcaat tctttttatt caggcgaaaa
    11461 attccccctg cgttattcca ttacgcccgt cattttgggg gtaagtgcgc cataatcatt
    11521 tattgttcgg taagtaatat tgcttcctgc atcagaaggc aatttaaccg tgctttcgcc
    11581 cattggaggc accaatgcat tttcaagaac ccgggttccg gcattcaact ctgttaccgt
    11641 caggtaatag ggtgtcgggt taatcagcgt cagggaattc gcgctacgac gaaatcttaa
    11701 tttttctgcg gcctgatcgg gtggcaacgc taatttagcc ggacgatagt acagtttaat
    11761 gcggctgata attgcgagct gtagcgtatt ctcagtcaat tttgatttat ccattgacgg
    11821 aatcgcttta acattcatcc agaataaact ttcccgatcc tgtggcaatt ggttattagt
    11881 tgcatcaaga atacgcaagg tattctctct ttttcccttc atcgcaaaca gaggaggcgt
    11941 cacgataaaa cgaccatcct ttacaccatc ggcattttcc acccatgatt gaattaaata
    12001 ggtactattt tcatcattat ttgtcacggc aagttgcact tgtttttgcc ctgccggata
    12061 aattacgcga gtcgcaccta aggccactcc cgcttcagcg cgtccggcaa ccatcattgc
    12121 catgaacatc aggatacctg ccagcaagca gaatgttatt tcctgcgatt tccttacatt
    12181 gacgttttta ttactcactg tcctgttcct gttatcacat tatctgctga acaattactg
    12241 ataggttaaa gagaaccagg cctgggcatt ggcgatgccg ccagtaaccc gacgcccggt
    12301 agcacgatat ttggcgatga aatgtagcga agtagagcct gagtaaagcc gtttccagtt
    12361 tgctggagga cgattaatcg gtacgaggtt tccttcatca tcaaacaacg ctacgccaat
    12421 attggtggct atccctggcc cctctcccac ggaaagcaca tccggatttt taccatccgc
    12481 gacaccgtga aacgccacac ccacacgttc actcaccacc gtgctacatt cccgtaaatg
    12541 aataacaaaa ggcaccggtg cgctatcttc ccctaccgca tgaaaccggt tactgctgat
    12601 ttgccccata ttgaccgtca tttgtttatc accggcttca atccggcaag tttccgcaat
    12661 aatgacgccc tgaaattgca tatttccgcc gggcagcgtg gtattccatt tatttccggc
    12721 cagagcaaac attggcagca acgacgctaa tagaaataat ctcttccttt tcatcgttat
    12781 ttttatcgca caagggtggg catccctgcc cgtaatgacg tccctgaacc tgggtaggtt
    12841 attgatactg aaccttgaag gtcgcatccg cattagcagc acccggggtt gcggcaccgg
    12901 ttgcaaaata acgcgcctgg aacggaatgg tgttggttcc gttattcagg gttgtttctg
    12961 aactaaatgt cgcaccgtcc agcgccagcg cagcacccgt tctgtccagg atctgcacac
    13021 caacgtttgt tgcgctaccc gcagctgaac tctgcagagc cagtacatca gtatgcgtag
    13081 catcaattgc cgtacctaag aaggcaacag cggcttttgt ggcaacagtg gtatcgcaat
    13141 cattcagctg aatgttaaaa ccaacggcag agctggttgc tccagcctgc ttcaggctag
    13201 cggtacgaac ctggcccaac tgaacggttt gatcaacaga gcctgcatca actgcgcaag
    13261 cggcgttaac aacttcccct ttaaagtgaa cggtcccacc atttaccgtc gtagtatcgg
    13321 ccagagccgc tgcggaactg agggacagag ccgacagaac aacgattgcc agagttttaa
    13381 ttttcatgct gctttccttt aaaaaactat ttctaaatcg acatgggcag tcgttctgta
    13441 cactttgttt tgtcaacgag tttgactgcc aacactgcac agttttcccc caaaagatga
    13501 aacatttggg gccattttga ctcatagagg aaagcatcgc gcacaaactt tttcagt
//
LOCUS       DAPGWD010000110        13396 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPGWD010000110 DAPGWD010000000
VERSION     DAPGWD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.111"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(565..1656)
                     /gene="ychF"
                     /locus_tag="RHX88_003163"
     CDS             complement(565..1656)
                     /gene="ychF"
                     /locus_tag="RHX88_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDV2231825.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1773..2357)
                     /gene="pth"
                     /locus_tag="RHX88_003164"
     CDS             complement(1773..2357)
                     /gene="pth"
                     /locus_tag="RHX88_003164"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV2231826.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2635..2913
                     /gene="ychH"
                     /locus_tag="RHX88_003165"
     CDS             2635..2913
                     /gene="ychH"
                     /locus_tag="RHX88_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDV2231827.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2968..4647)
                     /gene="dauA"
                     /locus_tag="RHX88_003166"
     CDS             complement(2968..4647)
                     /gene="dauA"
                     /locus_tag="RHX88_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDV2231828.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4772..5719)
                     /gene="prs"
                     /locus_tag="RHX88_003167"
     CDS             complement(4772..5719)
                     /gene="prs"
                     /locus_tag="RHX88_003167"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDV2231829.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5870..6721)
                     /gene="ispE"
                     /locus_tag="RHX88_003168"
     CDS             complement(5870..6721)
                     /gene="ispE"
                     /locus_tag="RHX88_003168"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDV2231830.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6721..7344)
                     /gene="lolB"
                     /locus_tag="RHX88_003169"
     CDS             complement(6721..7344)
                     /gene="lolB"
                     /locus_tag="RHX88_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDV2231831.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7558..8814
                     /gene="hemA"
                     /locus_tag="RHX88_003170"
     CDS             7558..8814
                     /gene="hemA"
                     /locus_tag="RHX88_003170"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDV2231832.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8856..9938
                     /gene="prfA"
                     /locus_tag="RHX88_003171"
     CDS             8856..9938
                     /gene="prfA"
                     /locus_tag="RHX88_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDV2231833.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9938..10771
                     /gene="prmC"
                     /locus_tag="RHX88_003172"
     CDS             9938..10771
                     /gene="prmC"
                     /locus_tag="RHX88_003172"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV2231834.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10768..11160
                     /locus_tag="RHX88_003173"
     CDS             10768..11160
                     /locus_tag="RHX88_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDV2231835.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11164..11973
                     /locus_tag="RHX88_003174"
     CDS             11164..11973
                     /locus_tag="RHX88_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDV2231836.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12009..12863
                     /gene="kdsA"
                     /locus_tag="RHX88_003175"
     CDS             12009..12863
                     /gene="kdsA"
                     /locus_tag="RHX88_003175"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDV2231837.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13012..13119)
                     /locus_tag="RHX88_003176"
     CDS             complement(13012..13119)
                     /locus_tag="RHX88_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV2231838.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3340 a         3378 c         3451 g         3227 t
ORIGIN      
        1 aacagcggtt aaaaaatttg gtgaagattt tttgtaataa tcctaattag ttatgggaat
       61 ttcgcttgat gtatctgcat aacaaaaaca atatgcgcca tttttgcatt actaataaga
      121 aaaagcaaaa aacatcgata ttcatttttg gtcaatgggt tttctggcct atcgttgtta
      181 ttgtgctact ggttatcatt ttcatcttgc caggatcgca cgtaatcacg ttattcgggg
      241 actttatctg cgggatatta cccttcaggt aagtcctttt ctcgccagca tattggagcg
      301 tgatcaatat tgatcagctg tgaagggggt gatgtgtagt ggaccaaaac ttacgttata
      361 tcactactaa tgagatatcc gtcatctgac actgatgtct ttaattattc tcgtggttca
      421 ctgtaggcaa taagcacaaa agtgtaggat gttacaagaa tgattaggac tcggtgaaat
      481 gaaaaatcca cgcaattgcg tggattatat atacttttgc gctcttcatg agatttagcg
      541 aaacctcatg agacaacaaa ttaattagac gttgaaaagg aagttcatca catcgccatc
      601 tttaacgatg taatctttac cttctgcacg cattttgcct gcttctttcg cgccttgttc
      661 acctttgtaa gtgatgaaat cttcaaacga gatggtttgt gcacggataa agcctttttc
      721 aaaatcagta tggattttgc ccgctgcctg cggcgcggtt gctccaaccg gaatggtcca
      781 tgcacgcact tctttcaccc cagcggtgaa gtaagtttgc aggttcagca gcttataacc
      841 ggcacggatc acacggttca ggcccggctc ttccagccca agctcctgca taaactcgtc
      901 acgttcttcg tcgtccagtt cggcaatgtc tgcttcaaca gcagcacaaa ccggaaccac
      961 aacagaacct tctttcgccg cgatttcacg cacctggtca agatatgggt tgttttcaaa
     1021 accgtcttcg ttgacgttgg cgatgtacat tgttggtttc agcgtcagga agctcaggta
     1081 acgaatagcc gctttctctt cagcgcttaa atccagcgcg cgcagcatac ctgcattttc
     1141 caactggggc aggcattttt ccaggaccgc cagctcagct ttcgcgtctt tatcgccacc
     1201 tttggctttc ttctgtacgc gatgaatcgc acgttcgcag gtgtcgaggt ctgccagcgc
     1261 cagttcggtg ttgataactt caatatcgtc agccgggtta actttgccgg aaacgtgaat
     1321 gatgttgtca ttttcaaagc agcgaacaac gtgaccgatc gcttcggttt cacggatgtt
     1381 ggtcaggaac tggttaccca gaccttcgcc tttcgatgcg ccttttacca gaccggcgat
     1441 atcgacaaat tccatggtcg tgggaagcgt acgttgcggt tttacgattt cagccagttg
     1501 atccaggcga gggtcaggca ttggtacgac gcctgtgttc ggctcaatgg tgcagaatgg
     1561 aaagttggcc gcttcaatac cggctttggt cagcgcgttg aacagggtag atttcccgac
     1621 attgggcaaa ccgacgatac cgcatttgaa tcccatgatt taaatcacct taatatctta
     1681 ataatcaacc tgttattgct aacagattgc agaaatggaa ataactttgc ctattataca
     1741 cggcactcgg caaaaatgcc gcagacaacg acttattgcg ctttaaaggc gtgcaatcgg
     1801 ttcgttgctt tggtcaagcc atctgtaaac cacatttcag tacaacgcgc cgcttcgtcg
     1861 atggcttcat caattaactt ctgttcacta acaggcggtt tgcctaacac aaaaccgaca
     1921 actttatttt tatcgcccgg atgaccgatt ccgatgcgta aacggtgaaa gttagggtta
     1981 ttacccaatt tactgatgat gtctttcagt ccattgtgac caccatggcc accgcccaat
     2041 ttaaatttgg cgacgccagg aggcagatcc agttcgtcgt gggccaccag aatttcgtcc
     2101 ggattaatgc ggaaaaaact ggccatcgcc gcaacggctt tgccgctgag attcataaat
     2161 gtagtcggga ctaacaggcg gacatcttcg cctccaagag tgactcgcga agtataacca
     2221 aagaatttag cctcttcgcg cagcggagcg cgcaaacgct ctgccagtaa gtcaacgaac
     2281 caggcaccag cattatgtcg cgttgcggcg tattcagcac cggggttcgc caggccgaca
     2341 atcaatttaa tcgtcacgtt tttttgtcct gagtgtgtac ataactggcg cgtagtttac
     2401 tggttgcggc cccgcttgac aaaaaactgc gtatcaaatg cagataacgt aataattgca
     2461 tgagtggact attagaaagt caaggtgttc aggcgtttat ttgtaaagtt ttgttgaaat
     2521 aagggttgta attgtgatca cgcccgcaca taacccacag ggtgttgtct atactttaca
     2581 cataaggaag aggggtattc cctgttacaa cccagaaagt tccggaggtg acatatgaaa
     2641 cgcaaaaacg cttcgttact cggtaacgtg ctcatggggt tgggtctggt ggtaatggtg
     2701 gtcggcgtgg ggtattcaat cctcaaccag ttaccacagt ttaatatgcc ccagtatttc
     2761 gcacatggtg cagtgctaag tattttcgtc ggtgccattc tctggctggc gggtgcccgt
     2821 gttggcgggc atgaacaggt gtgcgaccgt tactggtggg ttcgccacta tgacaaacgt
     2881 tgccgccgta gcgataatcg ccgtcatagc taacaaataa tgcagtttgc tgaccagtca
     2941 gtttgcgctg actggtcaat ctcgtactta taaatccgcc atcgccgcgc gacgattcgg
     3001 gaagaacgcc aggcgtcccg ggatcggttg aatgccagcg cgagccatag tgcgcagtgg
     3061 ctggaattcc acgttgcaca cgcgcagttc acatccctcg ggcagacgct tcacaaaacg
     3121 ctggaacgca tcaagaccac cagcatcaag taccggaacc gcatcccact tcagaatcac
     3181 aatccgtttg ccttcaagac gtgactccag gtccgtgaac aagccttcag cagcagcaaa
     3241 aaacagcggg ccaataacgc gcagaaccag gacatcgtct ggaacatcta cgactaccgg
     3301 tgccaggcga gtcatacgtg cgatacgacg cataaacagc agcgatgcca gcacgatccc
     3361 cacgctgatg gcaataacca tatcaaacag cacggtcagc gacatgcaca gcagcatgac
     3421 aatgatgtca tctttcggcg catgacgcag caagtcgacc actttgtgcg cttcactcat
     3481 gttccacgcc accatcaaca gcagggcagc catggcggaa agcggcagcc aggagagcag
     3541 cggtgccagt accagcaggg caagaataac cagaatagag tggatcaccg ccgagatagg
     3601 ggaagttgcc ccggcacgga cgttagcggc agaacgcgcg atggcagctg tagcggtaat
     3661 gccaccaaag aacggagcga tgatattccc cagtccctgt ccaaccagtt cgctattcgc
     3721 cttgtgtttc gtcccggtca taccatccag caccacggcg cagagcagag attcgattgc
     3781 gccgagcatt gccattgaga atgccgcagg cagcagtgtg cgaatagaat cccaggttag
     3841 cgtgaattct gaattaggca gatcccacgg cagcaccagt tgcggcagca gttgcggaat
     3901 accgttaccc tgagaaccat cggccaggac gtagtggaat tgcgaaccga tggtagcaac
     3961 atgtccgccg agcaggttaa caatccccat caccgcgcaa ccagccagca aggccggaag
     4021 gtgaccgggt aaacgaatgc ccagacgcgg ccaaaaaaca agaataccta gcgtcacaat
     4081 gccaatggca gcatcaccca cattaatggt cggcagcgcc ataaataatg cgccgacttt
     4141 ttgtagataa tgttccggga catgggccat ttgcagaccg agaaaatctt taatctgcat
     4201 ggtaccgatg gtgatcccga tacccgaggt gaaacctaag gtgacggaaa ccggaatata
     4261 ctcaatcagg cgaccaaagc gtgccagacc catcagaatc aaaaagatcc ccgacagcaa
     4321 ggtcgcaacc agcagtcctg ccagtccaaa ctgttgcgac acgggataga gaattaccac
     4381 aaatgccgca gtcggaccgg aaacgctaaa gcgtgaccca cccgtcagag caatgacaat
     4441 ccccgcaaca gctgcggtat ataaaccgta ctggggtgcc acaccactac caatagccaa
     4501 cgccatcgcc agcgggatag caataatccc gacggttatc ccggcaatca ggtcacgggt
     4561 aaaccgtgcg gcagtatatt tttctttcca gcaagcgtcg atcagagcgc ggaaaggcat
     4621 cacatgtgag gaaaatattt tgttcacaat aatgtttcat ccgtgagcgc atcatctgtc
     4681 aactaaatgg caggtgaagg aggcataggt catacaaatg gatattacag acaaaaaaac
     4741 ccgccgcagc gggtctttga gccgggttcg attagtgttc gaacatggca gagatcgatt
     4801 cttcgttgct gatacgacga atcgcttcgg ccagcatacc tgacagggtc agagtacgca
     4861 cgttcggcag tgatttgatt tcatcgctca gcggaatggt atcgcagaca acgacttcat
     4921 caattacaga gttacgcagg ttgttcgccg cgttgccaga gaagatcggg tgagtcgcgt
     4981 acgcaaatac acgtttagca ccacgttctt tcagagcttc agcagcttta cacagcgtac
     5041 cgccagtgtc gatcatatca tcgaccagta cgcagtcacg acctgcaacg tcaccgatga
     5101 tatgcatcac ctgggaaacg ttcgcacgcg gacgacgttt gtcgatgatt gccatatcgg
     5161 tatcgttcag cagcttagcg atagcgcggg cacgcacaac gccgccgatg tccggagaaa
     5221 ccacaattgg gttatccaga ttcagctgca gcatgtcttc cagcaggatc gggctaccaa
     5281 atacgttatc aaccggaacg tcgaagaaac cctgaatctg ttcagcgtgc agatccactg
     5341 tcagcacacg gtcaacaccg acgctggaga ggaagtctgc aaccactttc gcagtgattg
     5401 gtacacgagc ggaacggacg cgacggtcct ggcgcgcata gccaaagtag gggataacag
     5461 cggtgatacg acctgcggaa gcacgacgca gggcatcaac cataacgact aattccatca
     5521 ggttgtcgtt agtaggggca caagtggact ggatgatgaa aatatcacca ccgcgtacat
     5581 tttcattaat ttgtacgctg acttcgccat cgctaaagcg acctacagcg gcgtcgccga
     5641 gtgaagtgta caggcggttg gcaatacgtt gtgctagttc cggggtggcg ttaccagcaa
     5701 aaagcttcat atcaggcacg agaagaacct caggcatgcg tccattggtg gaaagaatct
     5761 gccgaaaact gtgcgggcca ggcatgatcc tttccaggcg gtgtattaaa gagcgcgatg
     5821 caacgtctgg aacaaggtga cgttgtcacc gaaactcagc ttgcccggct taaagcatgg
     5881 ctctgtgcaa tggggaaaga ttaacgcctt tcgccacaaa gccattgagc cattccgggg
     5941 cttgctctag cacctggcgg gcttcagact ctgtatcaaa ttcagcaaag acacaggccc
     6001 ctgtcccagt caggcgcgac ggggcgtatt ctaacagcca ggaaagcacc gcatcaacct
     6061 cgcgaaaacg ttttcttgcg ataacctcgc aatcattgct gaattcacat tttagcaacg
     6121 tttctattga cctttttggc gtattgcgcg ggagttcagg atctttaaaa atcaccggag
     6181 tcggaatact tacaccaggg tgcgccacca gataccactt ctctggcgga tccaccggcg
     6241 ttagtatttc accaacgcct tcggcaaacg cggcatgccc ccgaacaaag acaggaacat
     6301 ctgcgcccag cgtcagcccc atttccgcca gctcatccat gcttagcccg cattgccaga
     6361 gatgatttaa tgccaccagg accgtcgcgg cattggatga accaccgccg agaccgccgc
     6421 ccatcggcaa acgcttgtca atgctgatat tcgcaccgct tcccgtcgga agacgcccgc
     6481 tgtctgccgc agttttcatc aacaaccgcg ctgcgcgaac gatcaggtta tcttcatgtt
     6541 ccacgccttc aacgggcgtt aacagacgaa tatccccatc gtcacgaagc tcaatgctga
     6601 tggtgtcgcc gtaatcaaga aactgaaaca gcgtttgcag cgtgtggtaa ccatccgcac
     6661 gctgaccggt aatgtataaa aacagattaa gttttgccgg agagggccac tgtgtccgca
     6721 ttatttcact atccagttat ccatttttaa cttgatgcgt tgaccaccgt cggtgagttc
     6781 catattggct ggcatcgcag gttgcgtttt ggtgtcataa ccaccataaa caaccttcca
     6841 gtttttgcca ttctggctgt aggtaatttc gctcaggcgg tactggtcgt ccagtttgta
     6901 gtcggttgca tcacccggta aacctaaaat ccactggcgc aagctgttga gcggaattgg
     6961 cattccggtc aatttgccaa tcatctcttc ggcgtcatcg gcggtataac gctgaccttt
     7021 attgtcgact aactgcacgt tacccggttg agcattcagc tccagttccg tgctgcccaa
     7081 tgggttagtg agcagcagac ggtagcgatc ctggccggtt tgctgccaga aaaagcgggc
     7141 gtacactttt tgttggtcag aaatataagc gaacgcgccg cgagtctgat actgattaag
     7201 attgcgcacg tcttgctgat gctgacgcca ttgtggcgaa tccgggcttt tgccaggacc
     7261 tttgggcgtg gtaacggaac aggcagtgag cacaagagca gccagcggta gcaggcggat
     7321 aagacgaaaa tcgggcaggg gcatagtgat gacaagtcct tgagatacgt tgcagttata
     7381 acccttaatg ctagcgttac cgtccgctat cgtctatgtt caagttgtct taattgccag
     7441 aatctaacgg ctttcggcaa ttactccaaa agggggcgct ctcttttatt gatcttacgc
     7501 atcctgtatg atgcaagcag actaacccta tcaacgttgg tattatttcc cgcagacatg
     7561 acccttttag cactcggtat caaccataaa acggcacctg tatcgctgcg agaacgtgta
     7621 tcgttttcgc cggataagct cgatcaggcg cttgacagcc tgcttgcgca gccgatggtg
     7681 cagggcggcg tggtgctgtc gacgtgcaac cgcacggaac tttatcttag cgttgaagag
     7741 caggataacc tgcaagaggc gttaatccgc tggctttgcg attatcacaa tcttaatgaa
     7801 gaagatctgc gtaaaagcct ctactggcat caggataacg acgcggttag ccatttaatg
     7861 cgtgttgcca gcggcctgga ttcactggtt cttggggagc cgcagatcct cggtcaggtt
     7921 aaaaaagcgt ttgccgattc gcaaaaaggt catatgaagg ccagcgaact ggaacgcatg
     7981 ttccagaaat ctttctctgt agcgaaacgc gttcgcactg aaacagatat cggtgccagt
     8041 gctgtgtctg tcgcttttgc ggcttgtacg ctggcgcggc agatctttga atcgctctct
     8101 acggtcacag tgttgctggt aggcgcgggc gaaaccatcg agctggtagc gcgtcatctg
     8161 cgcgaacata aagtacagaa gatgattatc gccaaccgca ctcgcgaacg tgcccaaatt
     8221 ctggcagatg aagttggcgc ggaagtgatt gccctgagtg agatcgacga acgtctgcgc
     8281 gaagccgata tcatcatcag ttccaccgcc agcccgttac cgattatcgg gaaaggcatg
     8341 gtggagcgcg cattaaaaag ccgtcgcaac caaccaatgc tgttggtgga tattgccgtt
     8401 ccgcgcgatg ttgagccgga agttggcaaa ctggcgaatg cttatcttta tagtgtggac
     8461 gatctgcaaa gcatcatttc gcacaacctg gcgcagcgta aagccgcagc ggttgaggcg
     8521 gaaactattg tcgctcagga aaccagcgaa tttatggcgt ggctgcgagc acaaagcgcc
     8581 agcgaaacca ttcgcgagta tcgcagccag gcagagcacg ttcgcgatga gttaaccgcc
     8641 aaagcgttag cggcccttga gcagggcggc gacgcgcaag ccattatgca ggatctggca
     8701 tggaaactga ctaaccgctt gatccatgcg ccaacgaaat cacttcaaca ggccgcccgt
     8761 gacggggata acgaacgcct gaatattctg cgcgacagcc tcgggctgga gtagcagtac
     8821 atcattttct ttttttacag ggtgcattta cgcctatgaa gccttctatc gttgccaaac
     8881 tggaagccct gcatgaacgc catgaagaag ttcaggcgtt gctgggtgac gcgcaaacta
     8941 tcgccgacca ggaacgtttt cgcgcattat cacgcgaata tgcgcagtta agtgatgttt
     9001 cgcgctgttt taccgactgg caacaggttc aggaagatat cgaaaccgca cagatgatgc
     9061 tcgatgatcc tgaaatgcgt gagatggcgc aggatgaact gcgcgaagct aaagaaaaaa
     9121 gcgagcaact ggaacagcaa ttacaggttc tgttactgcc aaaagatcct gatgacgaac
     9181 gtaacgcctt cctcgaagtc cgagccggaa ccggcggcga cgaagcggcg ctgttcgcag
     9241 gcgatctgtt ccgtatgtac agccgttacg ccgaagcccg ccgctggcgg gtagaaatca
     9301 tgagcgccag cgagggtgaa catggtggtt ataaagagat catcgccaaa attagcggtg
     9361 atggtgtgta tggtcgtctg aaatttgaat ctggcggtca tcgcgtgcag cgtgttcctg
     9421 ctacggaatc gcagggtcgt attcatactt ctgcttgtac cgttgcggta atgccagaac
     9481 tgcctgacgc agaactgccg gacatcaacc cagcagattt acgcattgat actttccgct
     9541 cgtcaggggc gggtggtcag cacgttaaca ccaccgattc ggcaattcgt attactcact
     9601 tgccgaccgg gattgttgtt gaatgtcagg atgaacgttc acaacataaa aacaaagcga
     9661 aggcgctttc agtgctcggt gctcgcatcc acgctgctga aatggcaaaa cgccaacagg
     9721 ccgaagcgtc tacccgtcgt aacctgctgg ggagtggcga tcgcagcgac cgtaaccgta
     9781 cttacaattt cccgcagggg cgcgttaccg atcaccgcat caacctgacg ctctaccgcc
     9841 tggatgaagt gatggaaggt aagctggata tgctgattga accgattatc caggaacatc
     9901 aggccgacca actggcggcg ttgtccgagc aggaataatg gaatatcaac actggttacg
     9961 tgaagcaata agccaacttc aggcgagcga aagcccgcgg cgtgatgctg aaatcctgct
    10021 ggagcatgtt accggcagag ggcgtacttt tattctcgcc tttggtgaaa cgcagctgac
    10081 tgacgaacaa tgtcagcaac ttgatgcgct actgacacgt cgtcgcgatg gtgaacccat
    10141 tgctcattta accggggtgc gagaattctg gtcgttgccg ttatttgttt cgccagcgac
    10201 cttaattccg cgcccggata cggagtgtct ggtggagcag gcactggcgc ggttgcctga
    10261 acaaccttgc cgtattcttg atctcgggac gggtaccggg gcgattgcgc ttgcgctggc
    10321 tagcgagcgc ccggactgcg aaattatcgc tgtagatcgt atgcctgatg ctgtctccct
    10381 ggcacaacgt aatgcccagc atctggcgat caaaaatatc cacattctgc aaagcgactg
    10441 gtttagcgcg ctagccgggc agcagtttgc gatgattgtc agcaatccgc cgtatattga
    10501 cgagcaggac cctcatcttc aacaaggcga tgtccgcttt gagccgctca ctgcgctggt
    10561 tgcggcagac agtggaatgg cagacatcgt gcatatcatc gaacagtcgc gtaacgcgct
    10621 ggtatccggc ggctttctgc ttctggaaca tggctggcag cagggcgaag cggtgcgaca
    10681 agcatttatc ctcgcggggt atcatgacgt cgaaacctgc cgtgactatg gtgataacga
    10741 gcgcgtaacg ctcggccgct attatcaatg acaagttttt ctacactgct tagtgttcat
    10801 cttattagta tcgcgctttc tgttgggcta ttaaccttac gtttctggct acgttatcag
    10861 aagcatccac aggcatttgc tcgctggacg cgcattgtgc cgccggttgt cgatacgctg
    10921 ttactgttaa gcggtattgc gttgatggct aaagcgcaca tcctgccatt ttccgggcag
    10981 gcacagtggc tgactgaaaa gctgtttgga gttatcattt atatcgtttt gggttttatt
    11041 gcactcgatt atcgtcgtat gcacagtcag caggcgcgca ttattgcctt cccgctggcg
    11101 ttggtggtgc tgtacatcat cattaaactc gccaccacaa aagtaccgtt actggggtaa
    11161 gtcatgagat cgttagctga tttcgaattt aataaagcgc cattgtgcga aggcatgatc
    11221 ctggcttgcg aagcaatccg ccgcgatttt ccctcgcaag atgtttacga cgaactggag
    11281 cgtctcgtta gtctggcgaa ggaagaaatc agccagcttc tgcctttaga agagcagttg
    11341 gaaaaactga tcgcgctgtt ttacggcgac tggggattta aagcctcacg cggcgtttat
    11401 cgtctttccg atgcattatg gctggaccag gtgttaaaga atcgacaggg cagtgcggta
    11461 tcattaggtg cggttttatt atgggtcgcg aatcgtctcg atttgccgct gctgccggtg
    11521 attttcccta cgcagctgat attgcgcatt gaatgtccgg atggcgaaat ttggctgatt
    11581 aatcctttta acggtgaatc gttaagcgaa catatgctgg acgtatggtt aaagggaaat
    11641 atcagcccgt cggcggaact gttttatgaa gaccttgatg aagctgataa cattgaggta
    11701 atccgcaaat tgctggatac actcaaagcc tcgttgatgg aagaaaatca gatggagctg
    11761 gcgttacgca ccagcgaagc tttattacaa ttcaaccctg aagatcccta tgaaattcgc
    11821 gatcgcgggt tgatttatgc gcaactggat tgcgaacacg ttgcgttgaa cgatttaagt
    11881 tatttcgttg aacagtgtcc ggaagacccg atcagcgaaa tgatccgtgc gcaaataaat
    11941 aacatcgcgc ataaacatat tgtgctgcat taattaatcg acattttact caagattaag
    12001 gcgatcctat gaaacaaaaa gtggttagca ttggcgacat caacgtagca aatgacctgc
    12061 cgttcgtact gtttggcggt atgaacgtgc tggaatctcg cgatctggcg atgcgcattt
    12121 gcgagcacta cgtaactgtg acccagaaac tgggtatccc ttacgtgttc aaagcctctt
    12181 ttgacaaagc caaccgctcc tccatccact cttatcgtgg accgggcctg gaagaaggga
    12241 tgaaaatctt ccaggagttg aagcagactt ttggcgtgaa aattatcacc gacgttcacg
    12301 aaccaagtca ggcacagccc gttgctgatg tcgtggatgt gattcagttg ccggcgtttc
    12361 ttgctcgcca gactgacctg gttgaagcca tggcgaaaac cggtgcggta attaacgtca
    12421 agaaaccaca gtttgtcagc ccgggacaga tgggtaatat cgttgataaa ttcaaagaag
    12481 gcggcaacga aaaagtgatt ctttgcgatc gtggtgctaa cttcggctat gacaacctgg
    12541 ttgtcgatat gctgggcttc agcattatga agaaagtgtc tggtaactcg ccggtgattt
    12601 tcgacgtgac ccacgcactg caatgccgcg atccgtttgg cgcagcttcc ggtggtcgtc
    12661 gtgctcaggt ggctgagctg gcacgagccg gtatggcggt aggtctggcg gggctgttta
    12721 ttgaagcgca tccggatccg gaacatgcga aatgtgatgg tccatccgcg ctgccgctgg
    12781 ctaaactgga accgttcctc aagcagatga aagcgattga tgatctggtg aaaggtttcg
    12841 aagaactgga taccagcaag taatcttttt tgcttgaaaa ataaagtctt agcgttctgc
    12901 gttaagactt ttttcatggg tgccagatac aaaaaaggcc gcaggctgtt acccctgcgg
    12961 ccggtttcgg gcgcatattg ccatcacggc agcctgacgc ccgttttcac cttacttccg
    13021 gttacgccac cagccgacaa tcgctgcggt aataattccc gccaggatcg gtgctgccag
    13081 gtcgtgccag aaaatcatgg caaactgcgc gagcgtcata tagccgcctt gttgtaatga
    13141 caacattttg cggctattct tgaattgttc tggttcaaga ttagcccccg ttctgttgtc
    13201 aggttaaacc tctcaacgtg cgggggtttt ctctttccag caaccaatgc caccagggat
    13261 aaagcccccg caacattgcg cctcaccgga taacgccggc ttgatgtgga tactacgtct
    13321 caattcatct tcacttcatc cctgaaatgt ttgcaatgaa gagtgcattc cggtttttca
    13381 acagctgtta cagtcg
//
LOCUS       DAPGWD010000111        13246 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPGWD010000111 DAPGWD010000000
VERSION     DAPGWD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.112"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(248..460)
                     /gene="cspH"
                     /locus_tag="RHX88_003177"
     CDS             complement(248..460)
                     /gene="cspH"
                     /locus_tag="RHX88_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDV2231839.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            746..958
                     /gene="cspG"
                     /locus_tag="RHX88_003178"
     CDS             746..958
                     /gene="cspG"
                     /locus_tag="RHX88_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDV2231840.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            969..1157
                     /locus_tag="RHX88_003179"
     CDS             969..1157
                     /locus_tag="RHX88_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV2231841.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHVPLDFTK"
     gene            1132..1362
                     /gene="ymcE"
                     /locus_tag="RHX88_003180"
     CDS             1132..1362
                     /gene="ymcE"
                     /locus_tag="RHX88_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDV2231842.1"
                     /translation="MCRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            1352..1525
                     /locus_tag="RHX88_003181"
     CDS             1352..1525
                     /locus_tag="RHX88_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDV2231843.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1573..2646)
                     /locus_tag="RHX88_003182"
     CDS             complement(1573..2646)
                     /locus_tag="RHX88_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV2231844.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLATNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(2718..5462)
                     /gene="torS"
                     /locus_tag="RHX88_003183"
     CDS             complement(2718..5462)
                     /gene="torS"
                     /locus_tag="RHX88_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDV2231845.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5545..6573
                     /gene="torT"
                     /locus_tag="RHX88_003184"
     CDS             5545..6573
                     /gene="torT"
                     /locus_tag="RHX88_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDV2231846.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6546..7238)
                     /gene="torR"
                     /locus_tag="RHX88_003185"
     CDS             complement(6546..7238)
                     /gene="torR"
                     /locus_tag="RHX88_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDV2231847.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7368..8540
                     /gene="torC"
                     /locus_tag="RHX88_003186"
     CDS             7368..8540
                     /gene="torC"
                     /locus_tag="RHX88_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDV2231848.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8540..11086
                     /gene="torA"
                     /locus_tag="RHX88_003187"
     CDS             8540..11086
                     /gene="torA"
                     /locus_tag="RHX88_003187"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDV2231849.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAESIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            11083..11682
                     /gene="torD"
                     /locus_tag="RHX88_003188"
     CDS             11083..11682
                     /gene="torD"
                     /locus_tag="RHX88_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDV2231850.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(11775..12080)
                     /gene="cbpM"
                     /locus_tag="RHX88_003189"
     CDS             complement(11775..12080)
                     /gene="cbpM"
                     /locus_tag="RHX88_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDV2231851.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12080..13000)
                     /gene="cbpA"
                     /locus_tag="RHX88_003190"
     CDS             complement(12080..13000)
                     /gene="cbpA"
                     /locus_tag="RHX88_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDV2231852.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3245 a         3337 c         3453 g         3211 t
ORIGIN      
        1 ataaatctta gcaaaataca aattaaagat aaggaaattg ttacgaaagc tattaattat
       61 tgttggtaat atagtttcaa gtggtactat aactggctgt taaatggcat ttataactat
      121 taggtgcaga gatattcgct taaatggatt agtttctttg tggaaaagta actgatgtta
      181 taatgtatga tgttgactat tgcactattc caataaaacc agtcagcttt aaacaagcag
      241 cgtcatatta agagagataa acatttgccg ctgttggtcc tcgcaggcca tttacgcggc
      301 aaaattccac acgtaatcct ggtataagca cttctgcgtc gcggggagtg aatgcggaaa
      361 tatggacctg aacttcttta cgaccgtcgg aggggataat gaatcctttg ccgcttttgc
      421 gatcaaaggt tttgacaatt cctgtcattt tacgggacaa acaaattcct tactgaaaat
      481 actgcgctgc actatacggg gttaataaaa taaagccagc gatatttaag accgccggac
      541 ggctaaaata aaatttgctt aatctcaatt atcatgcgtt aatagctgcg tcggtttgaa
      601 agacagacag catacaaagt agtttactaa agcagttctc attatcaggc attatcccct
      661 tcttttgagt ctctctcctg aacactaagt agtttctgta ttaaagccct gtttgccgaa
      721 aggcccaaaa tgaaggaagt aaaatatgtc taataaaatg actggtttag taaaatggtt
      781 taacgcagat aaaggttttg gctttatcac tcctgatgat ggcagcaaag acgttttcgt
      841 ccatttcacc gccatccaga gcaatgaatt ccgcacgctg aacgaaaatc agaaagttga
      901 attttctatt gagcaggggc aacgtggccc cgcggcagcg aacgttgtta cgctctaagg
      961 ttgccgttat tactcaacat ctccatttcc gctgtccatg ttgtcatggt tcacagtacc
     1021 gcacatcggc attcgatgtg acggagcgaa accctttggg cgctaagtgt attttttgta
     1081 aatcgacgat gatcaccttt gataacgtcg cgctgcaaat acgcactgac catgtgccgc
     1141 tggatttcac aaaataatat caggctccct cgtggagcct tttttatatc tgccttattt
     1201 ttcttcaacg ctgtatgtat agtaagcgat aacctgttga ttattgaatc tttcggggag
     1261 atggcttata acatttctta cctgaccagg gtaccgggaa ccaacacctt actggcgtgt
     1321 tgctgtcttt caagaccaga agaggttaac agtgaatatt gaagagttaa aaaaacaagc
     1381 cgaaacggaa atcgccgact ttatcgcgca aaaaatcgcc gagctgaaca agaatacagg
     1441 gaaagaagtc tctgaaattc gcttcaccgc acgagaaaaa atgaccgggc ttgaaagtta
     1501 tgatgtcaaa atcaaaataa tgtgattttg tgaacatcac ccgtgcgagg tgatgttccg
     1561 cttgttgcta atttagtgac caatcattgg cgcttgtgga attaagcgtc ggtacaattc
     1621 atccggtacc gggctttgcc atactcccgc atacattgcg taaccaatca ccgcaaacat
     1681 aatccccaga accagtagcg tcattaacca gccagacaac gcaaaggctt ttttatttgc
     1741 cgcaggtttt tgcagtgaaa aggtcaatgt tgaggctacc ggacatgact ctacgcaagt
     1801 catacagccg gtacattcca ctgttcgtac ctgaattaat ttatcgaccg ggatccgtga
     1861 tgggcaattt tttgcgcatt tgccacagtc gatacaactt tcggcattgc gacgaatctt
     1921 aaacggcgac aatagcgaaa ccacgcccat cagcgcgcca tatgggcaaa gataacgaca
     1981 ccaggcatgg cgaataaaca ggctggcaat cagcaaaacg gtcacgctga ttaatgtcgc
     2041 ggtccccata tgacgaaaga aatcgagcat tttaacgtcc atcaccacgc tgtagggcga
     2101 caacataaaa tagtgaatcg cctgagcggg catcaataac gcgatataga gaaaaaaact
     2161 caacagcaaa tacttcacgc cgcgcagagg aatatccagc cagcggggaa ggacacattg
     2221 ccgaccaaac agtttgttac cgagatcgcc gattaattca gaaagcgtac caaccgggca
     2281 taaccatgag caaaaggcct ttttgagtaa tagactgatg acgataaaag cgaccaataa
     2341 cagcatcgcg gcggcgtgga cggacggtaa ctgacctgtt acaaggctat atttcagatt
     2401 catcagcccg gcaatcggta gccagccttc gatacctccc ggtctggcga caaatgtcgt
     2461 gctacttgcc gtttcgtaat agcgcaccca ataccagaac gtgatggcaa tataaatatt
     2521 cgttgccaac agtaataatt gcgtcgcttt acgccaggtc gtggcattac gccagtcatt
     2581 ccacggtaat ttgccgcccg tcgtgcctgg ccgccgctgc cagcgggttc ttttattctc
     2641 tgccatgatt ttgccagtcc gttaagttgt ataccaaatg ccactattct agttgttctt
     2701 aactggctga tattgattca aatcgcgttc aggtctttct tatgcaacca tgcttccaga
     2761 gcggcaacac tgcgtgtaat ttcttcgtgt ggaagggggg cagataatgg ctgctgctcc
     2821 agttgtgcgc atagctggct ggcgatacgc attcccagac tcgagcaact gctttttagc
     2881 tgatgcgcgg cacgctttat tttctcgcta tcctgactgg cgcgggcaat gtcgatttca
     2941 tcgagaagcg gcagggcatg ttgtgtaaat aataccagcc attcgtggat cttctccgtc
     3001 cccattaact gagcatcttc attgagttgc gatacatcca gcgattgatc gttattgact
     3061 tgcagttgga gatagtgcgc cagtaactga ccgagcactt cacgcggcac cggtttaggg
     3121 ataatcccgc ggaataatga actggtacgc tggcgcagcg tttcgtcaat gacatgggcg
     3181 ctaaagccaa tcaaaaccag cgacggatat tgctgtgcca gttgtcgggc aagcgtaatg
     3241 ccgtcgacat ccggcagatc aaaatccacc agtgcggcag caaacggttc gctattttgc
     3301 agtgtctcta aagcctgcgc ggcattgcca acagcaacaa cctgcgcacc actggtgttc
     3361 agcatctcaa cggtaattcg ctgggttagc gggttatctt caattaacag caaacgtaaa
     3421 ccgtcaagac gcaccgcctg attgactgtt tttggcacgg gtgccgtggc aacacgtaac
     3481 ggcaagcgta aacaaaaaca gcttccaacc tccggcgtgc tggtggcgct cagttcgccg
     3541 cccatcgcct gggccagacg gctactgata gtcagcccca gcccggtgcc gccgcgtttg
     3601 ccgcttacct gcacaaatgg ctggaagatt tctgccagtt tcgcgggatc aataccgcag
     3661 ccgctgtctt ccacttcgac cagccattgc tcgccatcag tgcgactacg caggataatg
     3721 tacccttcgt cagtaaaacg cagggcgttg ctcaacaggt tggttataac ctgacgaata
     3781 cgtcgtggat cgcccattaa cgcgcacggc atatcatcgg caattgccgt tgccaggcga
     3841 atcgggcgac ctttcacccg tccgctcatt aattgcaggg tactttccag cagcgggcgc
     3901 ggttcaaagg gctcatcgct gaccgaaaca ttcttgccac ctgcttcgat agcggaataa
     3961 tcgagaatat cgttgaggat ggtcagcaac gattcaccag agtcagtaat tgcccgcaaa
     4021 tcatcacgct gggcgttaag tgcggggtta tctgccagca gttgagcagt gccgagaata
     4081 ccgtacagcg gtgtgcggat ctcatggctc atcgccgcca gaaacgccga ttttgcctgg
     4141 ctggcttttt ctgcttccgc ccgtgcctgt cggtgttcta tcaccagttc ctgcaattca
     4201 gctgtacgcg ctttgacctg cgccgccagc tgttcgcggt ggcgattcag tgcatgaaca
     4261 ctgctgcgaa acgcatccat cagccgcccg atggtatcca gctcccgtac gccagcggtt
     4321 tccgggaaag gggagtcaat atcaccgtcc agcagccgtt gcagcgcctg cgtttgttcg
     4381 gcaagtggac gcgtgactga gcgataaacc acgcgccaaa ggatcagaat cagtgcgcaa
     4441 agtgaaacca tccccagcaa taacaggctg tattgcccgc gtgcactggc tttttccaga
     4501 tgcgccagtc cgtgctgatt acgcagctca atggtgtcga ccagctgact gacttcgcta
     4561 ctaaactgcg cgaactgggc gatgttattt tgtgcgagag tttgtaggtg attgctgatt
     4621 tcactgtcct gctgatacag cgccagcaaa tcgctatatt ggctaacggt agttaacgtt
     4681 gttgcgacct gcgcacgaac acccggatct tcaatgcgta tttgccgacg ttgcagaatt
     4741 ttcaccgcat tattgagctg cttttccagc attggtgcat ttttctggat ctgctccagc
     4801 cccagattca tcaccatttg ctgcacccgc agagcgctaa ggcgcagttc attcatctgg
     4861 ttaacatact caagatcgat atcaatcagc cgatcgagtg cactttcagc agcctgacgc
     4921 tgatcttgtt cgatcaaatc gtaaatcccg gcctgggtcg ctccagcgga agttgtcgca
     4981 ttattcgcct gaccttgcgc caggcgtgcg atctcatcgg cggcagcgac tatctgctga
     5041 ctgagttgcc gttgttgctg gcgtagttgc agacgccgcc ccaccagttc cccttgctga
     5101 cgtaatgaac gggagatctc ctgctcctgt tgttcaatag cggtggtatc aaaaccttgt
     5161 tcccgtaacg cttgcagcaa cgcattaatc ttcaggcttt gtgcggtgag cattcgcccc
     5221 tgcgcctgcc acatcttttc gttatcggca ctggtcaggt tctgcgcggc gaaaagttcc
     5281 caggcgctgg cttcgctcaa ctggcgcgcc atattcatgg taggaatcaa tgcctgagtg
     5341 ttgtcttttt ccacctggct gataaagcgc aggttgtacc atcccaccag ggtactggtc
     5401 agggttaaca gcgccatcag ggcaaagccc atccagagtc ttcgggtcag ggttaaattc
     5461 acggtcggtg cactttaggt gaaaaaggtt gagtcgcaaa gcagaatgca tctagcataa
     5521 agccttatta ttgatgaggc tatcatgcgc gtactgctat ttttacttct ttcccttttc
     5581 atgttatcgg cattttcggc tgataacctg ttgcgctggc atgatgcgca gcattacacg
     5641 gtgcaagcct ctacgccgct taaagccaaa cgcgcatgga aactgtgcgc gctttatccc
     5701 agcctgaaag attcatactg gttatcgttg aactatggta tgcaggaggc agctcgccgc
     5761 tatggtgtgg atttaaaagt gctggaggca ggcggctaca gccagttggc tacccagcaa
     5821 gcacaaatcg accagtgtaa acagtggggc gcagaggcca ttttgctcgg tagtagcact
     5881 acgtcatttc ccgacctgca aaagcaggta gcaaatctgc cggtgatcga actggtaaat
     5941 gctcttgatg atcccctggt gaaaagccgc gttggtgtac cctggtttca gatgggctat
     6001 caacctgggc gatatttggt gcaatgggcg cacggtaaac cactgaacgt gctgttgatg
     6061 cccggacccg ataatgccgg gggcagtaaa gagatggtcg agggttttcg cgcagccatt
     6121 gccggaagcc cggtgcgtat tgttgatatt gcgcttggtg ataacgatat tgaaatccag
     6181 cgtaacctgt tgcaggagat gctggaacgc cacccagaaa tcgacgtcgt tgccggaacg
     6241 gccattgcgg cagaggcggc aatgggtgaa gggcgtaacc tgaaaacgcc gcttaccgtg
     6301 gtgtcgtttt atctttcaca tcaggtgtat cgcgggctga agcggggaag agtgattatg
     6361 gctgccagcg atcaaatggt ctggcagggg gaactggcgg ttgagcaggc catcaggcaa
     6421 ttacaggggc aatcggtgtc tgataatgtc agcccaccga ttttagttct gacgccgaaa
     6481 aatgccgacc gtgaacatat tcgccgctcg ctgtcaccag ggggatttcg tccggtctat
     6541 ttttatcagc acacatcagc ggctaagaaa taaccttcac catgttgcgt caccagtaaa
     6601 tccgcgctga gtttatgacg taaacgacga attaacacat cgacggtgcg caggtcaggg
     6661 ttttccaccc gacgcgcaga aagcatacgt agcagacgtt cacggctgag aatttcgccc
     6721 ggattcgtca caaatgccac caacatttca tactctgcgc gggtcagttt aatcggctcg
     6781 ccatcccgct ccagcgtatg gcgcgacaca ttcaggcaat aaccggcaaa gcgatagcag
     6841 ttgtcctgag tgtgcggttg agcttgtcgc gcgaggtcga ttcgccagag cagatttttc
     6901 acccgtacta ccagttcgcg cagttccagc ggtttggtga cgtaatcgtc tgcgcccatt
     6961 tccagcccaa caatacggtc aatccgatcg ctgcgtccgg taaccagaat aatccccacc
     7021 gttgagcgtt ctcgcagggc gcgggttaac atcaggccat tttcatcggg taagttgata
     7081 tccagcagaa ttaaatctac cggctgattc tgcataattt cccgcagccc ggcaccgctc
     7141 gctgtaacgg aaacggtata cccctcctga gtgaagtaag attgtaatcg cgcctgggta
     7201 accggctcat cttcaacaat aacaatgtga tgtggcatca gagggtttta ctcattctgt
     7261 tcatatctgt tcatattctg ccgtaagccg ttcatcctga ccagtgccgc tgttcatatt
     7321 tgctcattaa gatcgcttca ctaaaccata attttacagg ggctattatg cggaaactct
     7381 ggaacgcgct acgccgaccc agtgctcgtt ggtcggtact ggcgctggtc gcaattggga
     7441 ttgtgattgg cattgcgctg attgtattgc cacacgttgg gatcaaagtc accagcacaa
     7501 ccgaattttg tgtcagttgc cacagtatgc aaccggtgta tgaagaatat aaacagtcgg
     7561 tgcatttcca gaacgcctcc ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg
     7621 atattccagg catggtgaag cgcaaactgg aagcgagcaa tgatatctac cagaccttta
     7681 ttgctcactc cattgataca cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc
     7741 gtgaatgggc gcgaatgaaa gaaaacaact cggcaacctg ccgctcctgc cataactacg
     7801 atgcgatgga tcatgcgaag cagcatcctg aagcggcacg tcagatgaag gtggcagcga
     7861 aagataatca atcctgcatc gactgtcata aaggtattgc ccaccagtta ccggatatga
     7921 gtagcggctt ccgtaagcag ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca
     7981 cgctgtactc tattgatatt aagccaattt atgcggcgaa aggcgataaa gaagcctctg
     8041 gttctctgct gcctgcttcg gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa
     8101 ttgaaattac cggctggacg gaaagcgccg gacgtcagcg tgtactcacc caattcccag
     8161 gtaaacgcat ctttgttgcc tcgattcgtg gtgatgtgca gcagcaggta aaaacgctgg
     8221 agaaaaccac cgttgccgac accaataccg agtggagcaa gttgcaggcc accgcatgga
     8281 tgaagaaagg cgacatggtg aacgatatca aaccgatctg ggcctatgcg gattcgttgt
     8341 acaacggcac ctgtaaccag tgccacggcg caccggaaat cgcccacttt gacgctaacg
     8401 gttggatcgg cacgctcaac ggcatgattg gctttaccag tctcgataaa cgtgaagaac
     8461 gcaccttgtt gaaatatctg caaatgaatg cgtctgacac cgcaggtaag gctcacggcg
     8521 ataagaagga agaaaaataa tgaacaataa cgatctcttt caggcatcac gtcggcgttt
     8581 tctggcacaa ctcggcggct taaccgtcgc tgggatgctg gggccgtcat tgttaacgcc
     8641 gcgccgtgcg actgcggcgc aagcggcgac tgacgctgtc atctcgaaag agggcattct
     8701 taccgggtcg cactgggggg ctatccgcgc gacggtgaag gatggtcgct ttgtggcggc
     8761 aaaaccgttc gaactggata aatatccgtc gaaaatgatt gccggattgc cggatcacgt
     8821 acacaacgcg gcgcgtattc gttatccgat ggtacgcgtg gactggctgc gtaagcgcca
     8881 tctcagcgat acctcccagc gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc
     8941 cctcgacatg ttctatgaag aactggaacg cgtgcagaaa actcacgggc cgagtgcctt
     9001 gctgaccgcc agtggttggc aatcgacggg gatgttccat aacgcttcgg ggatgctggc
     9061 gaaagctatt gccttgcatg gtaatagcgt tggtacgggc ggagattact ctaccggtgc
     9121 tgcgcaggtg atcctgccgc gcgtggttgg ctcaatggaa gtgtatgaac agcaaacctc
     9181 ctggccgctg gtattgcaga acagcaaaac cattgtgctg tggggctctg atttgctgaa
     9241 aaaccagcaa gcgaactggt ggtgcccgga tcacgatgtt tatgaatatt acgcgcagct
     9301 aaaagcgaaa gtcgccgccg gtgaaattga ggtcatcagc atcgatccgg ttgtcacatc
     9361 cacccatgag tatctggggc gcgagcatgt gaagcacatt gcggttaacc cgcaaactga
     9421 tgtgccgctg caactggcgc tggcgtatac gctgtacagt gaaaacctgt acgacaaaaa
     9481 cttcctcgct aactactgtg tgggttttga gcagttcctg ccgtacctgc tgggtgagaa
     9541 agacggtcag ccgaaagatg ccgcatgggc tgaaaaactg accggcattg atgccgaaag
     9601 cattcgtggg ctggcgcggc agatggcggc gaacagaacg caaattattg ctggctggtg
     9661 cgtgcagcgt atgcagcacg gtgaacagtg ggcgtggatg attgtggttc tggcggcgat
     9721 gctggggcaa attggcctgc caggtggtgg ctttggtttt ggctggcact ataacggcgc
     9781 aggcacgccg gggcgtaaag gcgttattct gagtggtttc tccggctcta cgtcgattcc
     9841 gcctgttcac gacaacagtg attacaaagg ctacagcagc accattccga ttgcccgttt
     9901 tatcgatgcg atcctcgaac cggggaaagt gatcaactgg aacggtaaat cggtaaaact
     9961 gccgccgctg aaaatgtgta tttttgccgg aactaaccca ttccatcgcc atcagcagat
    10021 caaccgcatt attgaaggct ggcgcaagct ggaaacggtt atcgccatag ataaccagtg
    10081 gacctcaacc tgccgctttg ccgatatcgt gctgcctgcg accacgcagt ttgagcgtaa
    10141 cgatctcgac cagtacggca accactccaa ccgtggcatt atcgccatga aacaggtggt
    10201 gccgccgcag ttcgaggcgc gcaacgactt tgatattttc cgcgagctgt gccgtcgctt
    10261 taatcgcgaa gaagccttta ccgaagggct ggacgaaatg ggctggctga aacgcatctg
    10321 gcaggaaggt gtacaacaag gcaaaggacg cggcgttcat ctgccagcgt ttgatgactt
    10381 ctggaataac aaagagtacg tcgagtttga ccatccgcag atgtttgttc gccaccaggc
    10441 attccgcgaa gatccggatc tcgaaccgct gggcacgccg agtggcctga ttgagatcta
    10501 ctcgaaaacc atcgccgata tgaactacga cgattgtcag gggcatccga tgtggtttga
    10561 gaaaatcgaa cgctcccacg gtgggcccgg ctcgcagaag tatccgttgc atctgcaatc
    10621 tgtgcatccg gatttccgac ttcactcgca gttatgtgag tcggaaactc tgcgtcagca
    10681 atatacggta gcgggtaaag agccagtgtt cattaacccg caggatgcca gcgcgcgcgg
    10741 tattcgtaac ggtgatgtgg tacgcgtctt taacgctcgc ggtcaggtgt tggcaggggc
    10801 agtagtttct gaccgctatg cacccggcgt ggcacgaatt cacgaagggg catggtacga
    10861 tccagataaa ggcggcgagc ctggtgcgct gtgcaaatac ggtaacccca acgtgctgac
    10921 catcgacatc ggtacttcgc agctggcgca ggcgaccagt gcgcacacta cgctggtgga
    10981 aattgagaag tacaatggag cagtggagca ggtgacggcg tttaacggcc ctgtggagat
    11041 ggtggcgcag tgcgaatatg ttcccgcgtc gcaggtgaaa tcatgaccac gctgacagca
    11101 caacagattg cctgtgttta cgcctggctg gcgcagttgt tctcccgtga actggacgat
    11161 gaacaactga cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc
    11221 gaaccgccgc tggctgcggc ggtgaatgag ctggaaaact gtattgccac gctgacagta
    11281 cgtgacgatg cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac
    11341 aaacaagcgg cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc
    11401 ttgttagttg aggcagggat ggaaaccagc ggcaatttca acgaaccggc agatcatctg
    11461 gcgatctatc tcgaattgct cagccatctg catttttcac tgggagaggg gaccgttcct
    11521 gcgcgaagaa tcgacagttt gcggcaaaaa acactgacgg cgctgtggca atggttacca
    11581 gagtttgttg tgcgttgtcg tcagtatgac agctttggtt tttacgcggc actaagccag
    11641 ttattgctgg tgttagtgga gagcgaccac caaaacagat aacgtcgttt gtgcgcctga
    11701 aaagacgcgt ttagcgtcgc atcaggcatt atggggcagt tgccggatgc gtcatcacat
    11761 ccggcaaaca cacatcacgg atgagctaca aaccgggaaa gtcgctggcg cagcaggcgg
    11821 ttttcctgct tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatcccaggc
    11881 cagtccagag ccagttcatg acgcaggcgt actgcgcgtt gcaccacgat ggcggcatgg
    11941 tcgtcaaata cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca
    12001 atttcattca actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa
    12061 gtcaccgtaa cattagccat tatgctttcc cccaatcttt acgtggatca aaagacgact
    12121 gggcgtctgc cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca
    12181 cgattttcag taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt
    12241 taacgcgcaa tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt
    12301 tcagtgttgg aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacta
    12361 caatttccag atcatggccg acaatatcga acagcggatg tggcgcaata tgaatcacca
    12421 gccacaaatc gccatttgga ccgccgtttt cgcccggcgt cccctggcct ttcagacgga
    12481 tgcgttgacc attgccgacg cccgccggga tcttgacatt cagcgttttc ggaatttcct
    12541 gttcgatcat gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct
    12601 cagtaagcgt ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgtgg
    12661 cggggcgttg acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa
    12721 aatcttcggc gttaaaactc tgaccgtcgc tatgatggaa ctgacggtta aattgcggat
    12781 cgttgcgatg ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt
    12841 cccaggcttc agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag
    12901 gatggtattt gcgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg
    12961 gtttcacgcc catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc
    13021 aacacaaatt gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa
    13081 tttcatatgc caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgcctcaag
    13141 gtggggtgct gaacatattt taaacaggtg aaaaagggtg agcgattttt gatagtggaa
    13201 ccaggcactt taagtttaac tggggcgtca ttatttatta aatttt
//
LOCUS       DAPGWD010000112        13147 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPGWD010000112 DAPGWD010000000
VERSION     DAPGWD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.113"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            403..1092
                     /gene="mutH"
                     /locus_tag="RHX88_003191"
     CDS             403..1092
                     /gene="mutH"
                     /locus_tag="RHX88_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDV2231853.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1161..1874
                     /locus_tag="RHX88_003192"
     CDS             1161..1874
                     /locus_tag="RHX88_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV2231854.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2012..2230
                     /gene="ygdR"
                     /locus_tag="RHX88_003193"
     CDS             2012..2230
                     /gene="ygdR"
                     /locus_tag="RHX88_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDV2231855.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2338..3378
                     /locus_tag="RHX88_003194"
     CDS             2338..3378
                     /locus_tag="RHX88_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDV2231856.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPTGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3410..4603)
                     /gene="lplT"
                     /locus_tag="RHX88_003195"
     CDS             complement(3410..4603)
                     /gene="lplT"
                     /locus_tag="RHX88_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDV2231857.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4596..6755)
                     /gene="aas"
                     /locus_tag="RHX88_003196"
     CDS             complement(4596..6755)
                     /gene="aas"
                     /locus_tag="RHX88_003196"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDV2231858.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7341..8372
                     /gene="galR"
                     /locus_tag="RHX88_003197"
     CDS             7341..8372
                     /gene="galR"
                     /locus_tag="RHX88_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDV2231859.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8379..9641)
                     /gene="lysA"
                     /locus_tag="RHX88_003198"
     CDS             complement(8379..9641)
                     /gene="lysA"
                     /locus_tag="RHX88_003198"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDV2231860.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDNIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9763..10698
                     /gene="lysR"
                     /locus_tag="RHX88_003199"
     CDS             9763..10698
                     /gene="lysR"
                     /locus_tag="RHX88_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDV2231861.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQQSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10685..11377)
                     /gene="ygeA"
                     /locus_tag="RHX88_003200"
     CDS             complement(10685..11377)
                     /gene="ygeA"
                     /locus_tag="RHX88_003200"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDV2231862.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLMEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11506..12924)
                     /gene="araE"
                     /locus_tag="RHX88_003201"
     CDS             complement(11506..12924)
                     /gene="araE"
                     /locus_tag="RHX88_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDV2231863.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSSNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         3359 a         3517 c         3210 g         3061 t
ORIGIN      
        1 gtaatttgag taatgaaaaa gagtaattcg tgacccaggt cacacctctc atttacgggt
       61 tggcgaaaga tcaaccaatg ccgtatttat ccacagaatg tgccactaag ttaagcactg
      121 aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt aaatggaaac agtagtggag
      181 gtttttcaca gttatcccag ctttctgtgg ataacatggt gtaagatcct gtttattttc
      241 agtgaccaga tttggaaaac ccgttgcagt gttgcgcaac tcgattaccg gcaacctaaa
      301 aagcaatata aatcagagaa ttgaacaaca catgtggaaa aagttacact gcgaatattc
      361 ggcacataat tgctgtttgt tttttaatca aggtatcatg acatgtccca acctcgccca
      421 ctgctctctc ctcccgaaac tgaagaacag ttgttagcgc aagcacagca actttctggt
      481 tatacattgg gagaactggc ggcacttgcc gggctggtta cgccagagaa tttaaaacgc
      541 gataaaggct ggattggcgt gttactggag atctggctag gtgccagcgc agggagtaaa
      601 cctgagcaag attttgctgc tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt
      661 ggtcgtccgc tggaaacaac attcgtttgt gttgccccgt taacgggcaa tagcggggtg
      721 acctgggaaa ccagccacgt gcgccacaaa ctcaaacgcg tactgtggat accagttgaa
      781 ggcgagcgca gcatcccgct ggcgcagcgt cgcgttggat caccgttgct gtggagcccg
      841 aatgaagagg aagaccgaca gctacgcgaa gactgggaag aattaatgga tatgattgtt
      901 ctcggtcagg ttgagcggat taccgctcgt cacggggaat atttacagat acgaccgaaa
      961 gcagcgaatg cgaaagcgct taccgaagcc attggtgccc ggggcgaacg gattctgacg
     1021 ctgccacgcg gcttttattt gaagaagaat ttcaccagtg cgctactggc ccgtcatttt
     1081 ctgatccagt agccatcgct ttgacctgcc gctttccggg catataatta ccgcttcatt
     1141 tttttggcag ggctttttag atgttatttg catggataac cgatcctaac gcctggcttg
     1201 cgctcggtac gctgacgctg ctggagatcg ttcttgggat cgacaatatt attttccttt
     1261 ctctggtggt ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt ctggggttgg
     1321 cgggagccat ggttatgcgt ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga
     1381 caaatccgct ttttacaata ttcagtcagg aaatttccgc ccgtgatttg attctgcttc
     1441 tgggtggctt gttccttatc tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg
     1501 aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg cagattatgc
     1561 tgctggatat tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc
     1621 tgtttattat gatggccgcc gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct
     1681 cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat gctggcgctc tctttcctga
     1741 ttctggtcgg ctttaccctg attctggaaa gtttcgacat ccacgtaccg aaaggttaca
     1801 tctacttcgc gatgttcttc tctattgcgg ttgaaagcct caacctgatt cgcaacaaaa
     1861 agaacccgct ctgatactcc gtacgctctc ctgcgggagg gcgtactcac tcctgttacc
     1921 ctcacagttt aagattttcc tgctttcaag atatataacg tcggtttata aacagactat
     1981 tatcataggt gggccaaatg aggataaccg gatgaaaaaa tgggcagtaa taatttctgc
     2041 agtcggactg gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga
     2101 tggccgtatg attttgaccg atggaaaacc tgaaattgat gatgataccg ggctggtgag
     2161 ttatcacgat cagcaaggta acgcgatgca aattaaccgt gatgatgttt cgcaaattat
     2221 tgaacgttaa caaataaggt cagcatccgg ctggccttaa gatttttctc tttccctttt
     2281 cccttccctc tgccattttt atattcctta tgtcgtgatt ataaaaagga aacggctatg
     2341 caatatcacc gtatacccca cagttcgctg gaagtcagca cgctggggct tggcacgatg
     2401 acgtttggtg aacagaacag cgaagccgac gcccacgcac aactcgacta tgccgtcgct
     2461 cagggcatta accttatcga cgttgccgaa atgtacccag tacctccgcg ccccgaaacg
     2521 caagggttaa ccgaaaccta cgtcggcaac tggctggcga aacatggcag ccgcgaaaag
     2581 ttaattatcg cctccaaagt gagcggaccg tcgcgcaata atgacaaggg catccgcccg
     2641 gatcaggcgc tggaccggaa gaatatccgc gaagcgttgc atgacagcct caagcgccta
     2701 cagactgatt acctcgatct ttatcaggtg cactggccgc agcgcccaac caactgcttt
     2761 ggcaaactcg gttatagctg gacggattct gcgcctgcag tttcgctgct ggatacgctg
     2821 gacgcactgg cagagtacca acgcgcggga aaaattcgtt atatcggcgt gtcgaacgaa
     2881 actgcatttg gcgtaatgcg ctacctgcat ctggcggaca aacacgatct gccgcgtatt
     2941 gtcaccattc agaaccctta cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa
     3001 gtcagccagt atgaaggggt cgaactgctg gcctattcgt gcctgggttt cggcacgctg
     3061 accgggaaat atctcaatgg tgcaaaaccc actggcgcac gtaatacgct ctttagtcgg
     3121 ttcacccgct atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt tgatattgcc
     3181 agacgtcatg gactggatcc tgctcagatg gcgctcgcgt ttgtacgccg tcaaccgttt
     3241 gttgccagca ctctgctggg cgcaaccacg atggagcagc tgaaaactaa cgtcgaaagt
     3301 ttgcatctgg agttaagcga agacgtatta gctgaaattg aagcggtgca tcaggtttat
     3361 acttatccgg caccataaga ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc
     3421 gctgccagat ccacagcgcc gttattgcca gcgcaaacag cgcaccgaag ccaatgccaa
     3481 tgggcacgac cgggatgcct accattaccg ccagcgagta aatgcccagc atcaacaaca
     3541 tggcgctgtt ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt
     3601 taccccgctc ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc atcacgccaa
     3661 tcagcatcaa caaggcatag gctggcagca gctcgtgttg cagggaaaaa atcagtacca
     3721 ccacgccaat caaaatcccg gctggcatac agcgtgacac agtttccagc gtaactaact
     3781 tcgctgccgc acctgcgcca accacgatac caatcgctac catcgcgttg agataggtgg
     3841 gcgtagcgtt atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca
     3901 gcgtgacacc cgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat
     3961 tgcgccatag cgaggtgcag gcattcagga aactgcgggt catgttgatg agattccagg
     4021 actgccccgg acgcgccgcc gccagtttgg gaatgtagat attggcaacg accgcaccac
     4081 cgtaggccag tgcgcatgcg gccagggcga cgaggacatg ccagtcagcc agcacaccac
     4141 cggctacgga accgagcaaa atcgccgcta tggtagaagc ttccattaaa ccgttagctt
     4201 tcactaactt actacccgtg gttaattcgc cgagaatacc gtatttcgcc ggtgaatagg
     4261 ctgcagcacc aacacccacc agcgtatagc cgagaaacgg attgatacca aagcagatac
     4321 tggctgcgcc cagcagcttc aggccgttgg caaacatcat cacccggcct ttggcgaagc
     4381 tatccgccac ctgcccgaca aacggcgcaa aaagaatgta agcacctaca aacaccattt
     4441 gcaggatggg ctggctccac tccggataga actgcgcttt cagtaacgcc agagtggcaa
     4501 acagtagggc attatcgcca aacgcagaga gaaactgcgc cacgataacc gctttcatcc
     4561 ccttcgacca caacgaagtg ttagtgtgca ctgactcact catcgtgttg ttccgcttcg
     4621 tctacccagc ttttcaacgt gacaaagtca ggtttgccgc tgccaagtaa tggcatctgt
     4681 ttcagatagc gaatatcgcg cggtacagca agctccggca cgccgtgctc gcgggcatac
     4741 tgttgcaact tatcgcgcgt cagttcgtta tctgtggtga aaagcaccag tgcctcgcct
     4801 ttgctggcat cgctcttaat cgcagtggca tggactttat ctggcgaaac accaagtgcc
     4861 agttgttcca ccatttccag cgacaccatt tcgcctgcaa ttttggcaaa gcgttttgcg
     4921 cggccctgaa tctgcacaaa gccctgctcg tcaaaacgca caatatcgcc agtgtcatac
     4981 cagccgcgct ccatttcgcc gcgaacattc tcggcggtgg gcacttccag tacacctggc
     5041 ttctccaccc gcagatagcc gttcattatg ttcggccctt tcagttgcag gcgtccgccc
     5101 tcttcgatac cagggaccga caacaggcgc gcatccattc ctggtagaat acgccctacc
     5161 gtaccgggtt tcgccgccat cggtacgttg atagaaacga caggcgcgca ttcggtcacg
     5221 ccgtagcctt caaggatgcg caggccaaat ttatcctgcc aaagctgttt ggtactttct
     5281 tgtaattttt ctgcgcctgc caccacatag cgtagacgat agaagtcata cgggttggcg
     5341 aaacgcgcgt agtgaccgag gaaagtcgag gtgccgaaca acacggtgca actgcggtca
     5401 tacaccaact ccggcacaat gcggtaatgc agcgggcttg gataaaggaa cacttctgca
     5461 cctgtaagca gtggcgtaaa caggcctacc gtcagcccaa aggagtgaaa cagcggtaac
     5521 gccgacataa agcgatcgtt ggtggtgaag tcggcaatcg ttttaatctg ctcgacattc
     5581 gccagaatgc ttttatggct atggacgacg cctttcggat ggccttcaga accggaggta
     5641 aaaaggatca gcgcctcttc ttccggctgc tgtttaacct gtgccagacg cggcatcagc
     5701 aaatgagcga agatccatac tttgtcggca gtggtgacat ctgcttttaa atcttccaga
     5761 tagacccagc gcacctgagt aagttgctcc ggcagatgcc agagtttgcc tttatcgaga
     5821 aactggcggg aagtgaagat ggttttgatt tcagccgccg taatagcact ggtcagccct
     5881 tttaccccgg cagtgtagtt catcattgcg ggcatgcggc gacgggcgat ggccccaaaa
     5941 atcactgccg cactgatgcc tgcattgggc agcattaagc cgatgcgttc gccttcaaca
     6001 ctgtattttt caaggatgcg tccaacaaac agcgttttcg taagcaattt gcgataggag
     6061 tctggggtaa agttgacgtc ttcgacacat ttcttcccgg ctccgaagcg gtacattgca
     6121 ctcagtaaag attcgtacag cgtttcacgc gggcgcaccg ccattcgcgc ttccatcatt
     6181 atttgatgca gcatttcgcc agcgattttg cgacggtcac gggcacgcgg cgcatccggc
     6241 atcgccacct gcgttggtgg caaaatatgc agagtaattt gcgggaacaa gcgacgttta
     6301 accagacctt tcaggcggct gaagtgcgta agttccgccc cttcaatacg cacaggaata
     6361 accgttgcac cagacttcgc cgcgacaaaa cccgcgccat cgtagatttt catcagcgag
     6421 cctgtagtgg tgatgcgtcc ttcagggaaa atcaccactg gtcggccctg ttccaccaga
     6481 cgtaccagat gtttaatagc cataggttgc gtcgggtcga gaggaacaaa gtcgataaat
     6541 gatttcagcc aacgcatata ccactgttgg cttattgagg tgtaaacggc aaacactgga
     6601 cgcacaggta aaaacagtcc aagcaaaatg ccatcaataa aagagacgtg attaggcgta
     6661 attagaacgc gctcgccctt cagtgcctgg gtgtcacccg taacgcgaac gcgatacaaa
     6721 acacggcaca aatttcgaaa aaagctaaaa agcattccaa ctccctttgc tctgattcag
     6781 taaaagcgaa tggagggaga ttacacgaga taaagaacgc gagcgacagt aaattaggtg
     6841 cgaaaaaaaa cctgcgcatc cgcgcaggtt ggtgcaagag acagggtacg aagagcgtac
     6901 cgcataatct caccaatcaa tacctctggg atcttgattg tggtctgcac gacgactctt
     6961 cgccagcgag aaaacgcaaa ggaatgaagg gaaatgcaac gaggtgtgta aattgtcggt
     7021 tactgttaca gattgatgac cggccaaaaa aaacctgcgc atctgcgcag gctggtgtaa
     7081 ttcatgtgct caacccgaag ttgacttcac ctatcaatac ctctgggatc accactttag
     7141 caacctgaag ccaaacgcca ccagcggtca atcgcaacag cgtttcgcaa agtgtaacca
     7201 aaggtttgca atctcttttt ttgtcttact tatttgcgtt tggctcacat tcccacgatg
     7261 aaaacacgcc accccttgaa ccaacgggcg ttttccgtaa cactgaaaga atgtaagcgt
     7321 ttacccacta aggtattttc atggcgacca taaaggatgt agcccgactg gcaggcgttt
     7381 cagtcgccac cgtttcccgc gtcattaata attcacctaa agccagcgaa gcttcccggc
     7441 tggctgtgca tagtgcaatg gagtctctta gctatcaccc gaacgccaac gcccgtgcgc
     7501 tggcgcagca gaccactgaa acggtcggtc tgatcgttgg tgatgtttcc gatccgtttt
     7561 tcggtgcaat ggtgaaagcg gtcgaacagg tggcttatca caccggtaat ttcttattga
     7621 ttggcaacgg ttaccacaac gaacaaaaag agcgtcaggc tattgagcaa ctgatccgcc
     7681 atcgctgtgc tgcgttggtc gtccatgcca aaatgatccc ggatgccgat ttagcctcat
     7741 taatgaaaca aatgcccggt atggtgctga tcaaccgtat cctgcctggc tttgaaaacc
     7801 gttgtattgc tcttgacgat cgttacggtg cctggctggc aacgcgtcat ttaattcagc
     7861 aaggtcatac ccgcattggt tatctgtgct ctaaccactc tatttctgac gccgaagatc
     7921 gtctgcaagg gtattacgat gcccttgctg aaagtggtat tgcggccaat gaccggctgg
     7981 tgacatttgg cgaaccagac gaaagcggcg gcgaacaggc aatgaccgag cttttgggac
     8041 gaggaagaaa tttcactgcg gtagcctgtt ataacgattc aatggcggcg ggtgcgatgg
     8101 gcgttctcaa tgataatggt attgatgtac cgggtgagat ttcgttaatt ggctttgatg
     8161 atgtgctggt gtcacgctat gtgcgcccgc gcctgaccac cgtgcgttac ccaatcgtga
     8221 cgatggcgac ccaggctgcc gaactggctt tggcgctggc ggataatcgc cctctcccgg
     8281 aaattactaa tgtctttagt ccgacgctgg tacgtcgcca ttcagtgtca actccgtcgc
     8341 tggaggcaag tcatcatgca accagcgact aaccgcagtt aaagcaattc cagcgccagt
     8401 aattcttcga tggtctggcg acggcgaatc aaccgcgcct gaccattatc aaacagaact
     8461 tctggtaaca gcggacggct gttgtagttg gatgacattg atgcgccata tgcccctgta
     8521 tcatgcagta ccagataatc acccgccttc acttccggca aggcgcgggt ttcaacattt
     8581 cccccttcct gctgggtaaa gacatcgccc gattcacata atggcccggc gacgacggtt
     8641 tccaccgttg gcgcgtgttc cagagaacga ccatcagctg ccagggcact gatatggtgg
     8701 taactaccgt acattgccgg gcgcatcaga tcgttgaacc cagcatcaac cagcacaaag
     8761 tggcggctac ccatttgttt gacgctccgc acctgagtaa ttaatacgcc agactgcgct
     8821 accaggaagc gacccggttc aatttccagt ttcacagggt ggcccaaatg gcgagcgatt
     8881 tgctcacgcg cggcattcca cagaccataa taatgttcgg tatcaaccgc ctcttcaccc
     8941 tgttgataag gaatagaaag cccaccgccc gcagaaatag cctgtaaatc ctgaccgaat
     9001 tcgatgacct gacgcaccat agcaccacac acctgttcca gatgggcata atcaacgcca
     9061 gaaccaatgt gcatgtgaat gccgaccagc tgcagatgat gacgttgtat cacgtccagt
     9121 gcggcgggca gatcggtgta ccagataccg tgcttgctgt tttcgccacc ggtattggtt
     9181 ttttggctat gtccgtgacc aaaccccgga ttaacgcgca gccatacccg atgccctggc
     9241 gaaacctggc ccagttggtc gagcatatca acagaacccg cattcaccgg aatttgcaat
     9301 tcactgacgc gttcaagcgt cgcctgatcg ataacatctg ccgtaaaaac aatattatcg
     9361 gggtgcgttt gcggattgta acccgccgcc aacgcacgct ctatttcgcc taacgagacg
     9421 gaatccactt tcacgccctg ctcacgcatt aagcgcaaaa tatgaatatt ggaacaggct
     9481 ttctgtgcaa agcgcaccac atcaaactgt ttcagcgctg caatctgccg acgaataatt
     9541 tgcgcatcgt agacccacac cgggcagcca aattcagcgg gcaaacgcag cagattttcg
     9601 gcggtgagat cggtatcggt actgaacagt gaatgtggca taacaaactc cagataagtg
     9661 cttttttatg attacgccac atcataaaaa gaataaaaaa tatcgattta tgtcgagtct
     9721 atgcaaaaat gatatggatt accggattgc gagagagcgc taatggccgc cgttaactta
     9781 cgtcatattg aaatttttca tgcggtaatg accgccggaa gcctgactga ggcagcacac
     9841 ctgctacaca cctcacagcc aaccgtcagc cgcgaactgg cgcgctttga gaaggtgatc
     9901 gggctaaaat tgtttgagcg cgtacgtgga cgattacatc ctaccgtgca aggactgcgt
     9961 ctgtttgaag aagtgcaaca atcctggtac ggactggatc gcattgtcag cgccgcagaa
    10021 agtctgcgcg agtttcgcca gggagaactg tctattgcct gcctgccggt cttttcgcaa
    10081 tcttttttac cgcagctcct gcaacccttt ctggcacgtt atcccgatgt cagcttaaat
    10141 atcgtgcccc aggaatcacc gctacttgaa gagtggctct cggcccagcg tcatgattta
    10201 ggactcactg agacactcca tacgcctgcg ggaacagaac gtaccgaatt actctcttta
    10261 gatgaagtgt gtgtgttacc tccgggtcat ccgctagcgg taaaaaaggt attaacgccg
    10321 gatgattttc agggtgagaa ctacatcagc ctttcccgta ctgacagcta tcgccagttg
    10381 ctggatcagc tatttactga acatcaggtt aaacgacgca tgatcgtaga aacccacagc
    10441 gccgcgtcag tctgcgcaat ggtacgggcg ggggtaggtg tttcggtggt taacccgctc
    10501 accgcactgg attatgcggc aagtggttta gtggtgcggc ggttcagtat tgcggttccg
    10561 ttcaccgtca gcctgatccg ccccctgcac cgcccgtcat cagcgctggt gcaggcgttt
    10621 agtgagcatt tacaagcggg gttaccgaaa ctggtcactt ctcttgacgc tattttgtcg
    10681 tcagctacga cagcataaaa gcgacagcat cctcggcatg gatcgccgca gtatcaaaca
    10741 caggcaaaac actgcgctct tctggcacca gtaaaccaat ttctgtgcag ccaaaaatga
    10801 cgccctgtgc gccctgttct gcaaggcgag caatcacttg cgcataataa gcgcgtgacg
    10861 cttcggtaaa ttgccccaga cacagttctt caaaaataat ctgattaatt ttcgcccgtt
    10921 catccgcttc aggaataaga caattgatgg aaaattgttc catcagccgc ccgcgataaa
    10981 aatcctgttc catggtgtaa cgcgtaccca gcagcgccac acgagtcatt ccggccccgg
    11041 taattgcacg tccggtggca tccgcaatgt gtaagaaagg cagagtgcaa cgtgactcaa
    11101 tggcatccgc cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc
    11161 gctgtaagcc aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac
    11221 gctggcactc ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa
    11281 gcccaccaag ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt
    11341 cccagctcat tcctcccagc aaaccaattg ttttcattcc tctctcctga tgtgtgttac
    11401 cgcaacaagg ctgaataagg aacgagacaa acgcctcaac ggccaggtgc ccgatctcta
    11461 ttaacgaaaa aagggccgga tgtacagcac atccggcccg tgaaatcaga cgccgatatt
    11521 tctcaacttc tcgcctgcca tcagtttgcg ttcgatatgt tccagcgtga catttttggt
    11581 ttccggaatg agccagaaag tgatgcccac aaacgcaatg ttcagcgcag tgtagagcca
    11641 gaacgtaccg gcagcgccaa tgctatcaag cagtgtcagg aaggtcgcgc cgataatcat
    11701 attcgacacc cagtttgtcg tcgtcgaaca ggtaataccg aaatcgcggc atttcagcgg
    11761 ctgaatttca gagcacagga tccacaccac tggcgcggcg ctcatcgcat aaccggcaat
    11821 acacatcatc gtcatgccaa cagagagcca ggacaagcca ctggaagccg taccgttatc
    11881 aaactgcatc aggcaatagc ccagcaccag agtgcctaac gccatcacgc tgaaaccaat
    11941 tttcagagcc ggtttacgac ctgctttatc taccgtaaag accgcaatga aggtcgcgaa
    12001 cataaaggtc agtcccacga ccagagtcgc aatcatctgt tgttctgtgg tcgtaaagcc
    12061 cgccattttg aagatacgcg gcgcatagta catgatgatg ttcataccgg taaactgctg
    12121 catcgcctgc aacaacatac cgagaaacac agcacgacgg acgttacggt tgatcttaaa
    12181 cagtgcccaa ccgccttgtt ttaacttcag gctttcacga atttcgttga gttcttctcg
    12241 cgctttttcc gacgtatcgc gcagcatacg caacacttct tccgcctcaa tatgacgccc
    12301 cttttccgcc agccagcgcg ggctatttgg caggaagacc accagaataa tcagcaggac
    12361 tgctggtaaa gcaagaaccc ccaacattgc gcgccagtta ctgctataac tgaacgctgt
    12421 atcggataaa aacgccagca cgatgccgag tgtgaccatc aactggtaca tactgatcat
    12481 cttaccgcga acgttttcac ttgccatttc agaaagatac agaggagcgg tgtaagacgc
    12541 gatcccgaca gcaatgccca gcaccacacg agcggcgatt aacatctcta cgctggtcgc
    12601 aaaagcggac cctatagaac cgagtacaaa caggatggcc cccgccatca ggctgtattt
    12661 acgacccagg cggaacgaca gccaaccatt aaacagcgca ccaattgctg cgccgagcat
    12721 catgctacta accacccatt cctgcaaacg actggtcagc acaaagtgat cggtaatgaa
    12781 cggcaacgct ccggcgatta cgccgatatc aagaccaaat aacaatcctg cgaccgcagc
    12841 agctaccgaa acaaacatat tcatacgccg cgtatcccgc aaagaacgtg gcgttaaagc
    12901 agattccgta ttgatagtaa ccattttttc ctgccagcag agagtaagac atagtgaaaa
    12961 aatacgtgaa caactcacgc aggtgtcagg tcggaaacag cataaatatg gattaaattg
    13021 ctgcgacatg tcgttatgtg atggatattc caattttcaa attaagttga attattgaga
    13081 ttattattaa ccacctaatt ttacagcaga taaaattcat aaagttcatt aattgataat
    13141 taatatg
//
LOCUS       DAPGWD010000113        13145 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPGWD010000113 DAPGWD010000000
VERSION     DAPGWD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.114"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..629
                     /locus_tag="RHX88_003202"
     CDS             <1..629
                     /locus_tag="RHX88_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV2231864.1"
                     /translation="SAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPY
                     VNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVIC
                     TGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEEN
                     IPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(783..2432)
                     /locus_tag="RHX88_003203"
     CDS             complement(783..2432)
                     /locus_tag="RHX88_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDV2231865.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2583..3932)
                     /gene="ghxP"
                     /locus_tag="RHX88_003204"
                     /gene_synonym="yjcD"
     CDS             complement(2583..3932)
                     /gene="ghxP"
                     /locus_tag="RHX88_003204"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDV2231866.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLRDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4478..4942)
                     /gene="soxR"
                     /locus_tag="RHX88_003205"
     CDS             complement(4478..4942)
                     /gene="soxR"
                     /locus_tag="RHX88_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDV2231867.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5028..5351
                     /gene="soxS"
                     /locus_tag="RHX88_003206"
     CDS             5028..5351
                     /gene="soxS"
                     /locus_tag="RHX88_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDV2231868.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5354..6940)
                     /gene="pdeC"
                     /locus_tag="RHX88_003207"
     CDS             complement(5354..6940)
                     /gene="pdeC"
                     /locus_tag="RHX88_003207"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDV2231869.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7370..7651
                     /locus_tag="RHX88_003208"
     CDS             7370..7651
                     /locus_tag="RHX88_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDV2231870.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7750..8286)
                     /gene="ssb1"
                     /locus_tag="RHX88_003209"
     CDS             complement(7750..8286)
                     /gene="ssb1"
                     /locus_tag="RHX88_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDV2231871.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8540..11362
                     /gene="uvrA"
                     /locus_tag="RHX88_003210"
     CDS             8540..11362
                     /gene="uvrA"
                     /locus_tag="RHX88_003210"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDV2231872.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11397..11753)
                     /locus_tag="RHX88_003211"
     CDS             complement(11397..11753)
                     /locus_tag="RHX88_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV2231873.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11757..12173)
                     /locus_tag="RHX88_003212"
     CDS             complement(11757..12173)
                     /locus_tag="RHX88_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDV2231874.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12284..12997)
                     /gene="aphA"
                     /locus_tag="RHX88_003213"
     CDS             complement(12284..12997)
                     /gene="aphA"
                     /locus_tag="RHX88_003213"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDV2231875.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3410 a         3453 c         3209 g         3073 t
ORIGIN      
        1 gctccgctat gcatgttcaa ttctacaatg cagcaatgaa tagagccaat attaaaaaca
       61 ccttccttga ctattcaaat ttttatatgg cgtacatggc tgaggtaaat ctttataaag
      121 taatagcgcc atatgttaat ttatttaaag ccgaccttag tttctctaaa ctcgatttaa
      181 ttaactttga acatgctgat ctgtctcgcg tcaatctgaa caaagcaatc ctccagaata
      241 taaacttaat tgatagcaaa ctcttttgta cgtggctgac aaatacattc ctcgaaatgg
      301 ttatatgtac cggctctaat atggctaatg ttaattttaa taatgccaat ttaagcaact
      361 gccatttcaa ctgttctatt ttaacaaaag cctgtatgtt taatacccgt ctctatcggg
      421 ttaattttga tgaggctagc gtccagggaa tgggcatttc cattctccgt ggggaggaaa
      481 atatccccat tgatagtgat accctggtaa cactacagaa attctttgaa gaagattgta
      541 cctctcatac tggcatgtca caaactgagg ataatattaa tgcagtcgct atgaagatta
      601 ctgcagatat tatgcaacac gcagattgag gttaagtcga agaacagcca atgaattgac
      661 tttacaagag agataactta tttgttttag aatcatgaat gttagcccga gctaacctgt
      721 taaaggtgca aaagattgca cctttttcag attacctcgg gcctggttac gtcgctttcg
      781 gcttactgat tttcctcaat tagcaacgct tcaagcaaat cgagatcgtg cagtaatttt
      841 tgcagcgttt cgttgctgat ctcccgcgtg gcgcgcaggt ggtaaagttc agcacgttca
      901 gaacgcaatg ccgccagacg gaagcgacgc tcaaggttct cttcctgcac ggaactttca
      961 acgtcgttac gtccatcggc gcgacgacgc aggttaccaa tgacgcgaga actgacctcc
     1021 gtaagcagct ggttatcgat attctcttcg gtatccgccg ccagacgctc ctccattttc
     1081 tggatcgcca caatcgccac ttctgccgtt gccgctcgcg caatacgttc ctctttcaat
     1141 tgttgcgaat ggtccgcgac ttcaatgtgt tgtagcagaa taggcaacat caccacgccg
     1201 acaaacagcg agaagagaat gacgccagcc gccaggaaca ccagctcata gcgcgccggg
     1261 aagacattac catccggcaa gagcagcggg atagagagca caccggccag agtgatcgcc
     1321 ccacgcaccc cggcgaaaga cgcgatcagg atttctcgtg tggtccacga accaaactcc
     1381 atcggcttct tcttcaggaa gcggttgctg aactttttca tcgtccacag ccagccgaaa
     1441 cggaccagca tcagcgccgc atatatcaga ataatatcgg taaacagcat ccagatttcg
     1501 acgttagggt cgatttctgc tgccatcagc gacgtctcca gaatacccgg cagctgcaga
     1561 cctaacagca ggaacaccat gccgttaaag acaaattcca gcatcgccca ggtgctgttt
     1621 gcacgcaggc gcattgccag cggcgcacgg cgcatcacac cggagcgggt gatggtcatc
     1681 ccggcggcaa ccgcagcgag gatgccggaa acgccaatat gttcggcaat cagataagaa
     1741 gcgaatggca gcaagaacag cagaacaatc tgcgtcgcgg gttcatcacc gccccagcgg
     1801 ctgaggaatc gcagcgagcg accatacagc cagctcacca caaaaccggc aagaataccg
     1861 ccaatggcta ctttcataaa ttcgaccgtc gcaccgccga cggtgaagat catcgtcccc
     1921 atcgccactg ccacggcaaa cttcaacgac accaggccgg aagcgtcatt catcaacgct
     1981 tcgccctgca aaatgcccat gatttttttc gggatgcgcc cttcgccaac aatcccggag
     2041 agcgccacag catcggtcgg agaaagtacc gccgccagcg caaaggcggg gatcagcgga
     2101 atgcccggca ccacccagta aataaggaag ccaatgccga ccacggtgac caccaccagc
     2161 gccagcgcga ggccgaaaat ctctcgacca tgttcgagaa attcacgggt cggcgttttc
     2221 cagccatcag cgaacagcaa cggcgggata aacaagacta aaaagagttc aggatcaaac
     2281 tccacatgca aaccaaacgt cggccacgcc agtagcgcac cgatggcgat ttgcataagc
     2341 ggaagcggga tctgaaaggg catgacacga gtgaccaccc cggacagcga gaccacgagg
     2401 gtcattatca gtatggtgaa gaagatttcc atgcgttccc tgtttgcgat gttttttatg
     2461 atctgagaaa gacgttatgc ctgaattctc attttttcag aatatcgcaa aagctcacac
     2521 aatgtgcgga gaaaataaaa acgggcagcc attgccaccc gttttcagaa tccgatgaaa
     2581 gattagatag cccagccacc cgcatagaag gtcaccagcg ccacggcgat aacgaccgta
     2641 ccgatgttca acttgcgcca ttcgccggaa accagacgac caatcaccag agtcgcgaag
     2701 ccgatcatga tgcctgttac gatgttacag gtcagcacga tgaataccgc cgtaaccaga
     2761 cccgccatcg catcaacaaa atcagcaaag tcgattttcg ccacgttgct cagcatcagc
     2821 aggccaacgt acatcagcgc cggagccgtt gcataccccg gaacgaggta agagagcgga
     2881 gagaggaaca gaatcagcag gaacagcacg ccaacggtga tagccgtcaa accggtttta
     2941 ccgcccgccg ccgtacccgc cgcagactcg atgtataccg ctgccggagc tgcacccacc
     3001 aggccagaga aaacgctgct catggagtca gtggtcagtg ctttcccacc gtcgataatt
     3061 tgcccatctt tatccagcag gttcgcctgg ccggcgacgg cacggatagt tccggtggca
     3121 tcaaatactg ccgtcattac cagcgccaga acgcttggca ggactacagg attcagcgcg
     3181 cccataatgt ccaggctgcc aatcagtgag ttgccgtttt catccctcaa tgaaggcatg
     3241 gcgaaaacgc cggagaaatg gacgttagga tcgaagatca aaccgacaat tgagataccg
     3301 ataatggtca gcagaatgcc accagggact ttcagttttt ccaggccgat gatcaccgcc
     3361 agacctacca gtgacataat caccgggaag gtcgcgaaat cacccagcgc aacgggcaga
     3421 ccatcaagcg ggtttttaat caccagaccg acaccgttag cggcaatgag cagcaggaac
     3481 agaccgatac caatccccgt gccgtgcgcc acaccgtgcg gcaagttgcg caaaatccag
     3541 ctacggatac ccgtggcaga aattaccgta aacagcacac ccatcaggaa cacggcaccc
     3601 agcgcgacag gtacgctaat atgttgcccc agcaccaggc tgaatgcggt aaatgccgtc
     3661 agggagatgg cgcaaccaat cgccaacggc agattagccc acagacccat cacgatagaa
     3721 ccgagtccgg caaccagaca ggttgcaacg aaaactgccg caggcgggaa gcctgcttta
     3781 cccaacatac ctggaacgac gatgaccgag tagaccatcg ccagaaacgt tgttaacccg
     3841 gcaaccactt cctgacggac agtgcttcca cgttgtgaaa ttttaaacca ggcgtcgagt
     3901 gaaccgccgg tacgcgctga tggcgtagac ataagaaaac atcccctgag agtttaattt
     3961 tcgtcaagat gcgtggcgga caatcccctg ccagcgaaac gttatcttcc tgctccaggt
     4021 ttgcgacaat ttgctgcgtc gcaaaaaagc aaacgtttag ctccgcgcat acaaaacggg
     4081 tggtaaaaaa agcaaacgat tatccggcga tcatagcggc tttttcaacc gaagtttgct
     4141 gcttttttct cttttattgt cttgacgatg aaaaaccagg caattgatgc gcaaacgtta
     4201 tcgtcggtgc gcaagattgc agcgttatcg agccgtatac cgttcaggat tgcgataaaa
     4261 aatcgtctga gatgacaaaa atattgcggt taaaaagtat acttttacac tggcgaagaa
     4321 acgtcggtga catacaatta aagcatcaac accaaccgga acctccacca cgtgctcgaa
     4381 tgaggtgtgt tgacgtcggg ggaaaccctc ctgtgtacca gcgggataga gagaaagaca
     4441 aagaccggaa aacaaactaa agcgcccttg tggcgcttta gttttgttca tcttccagca
     4501 agcgtgcgcc ggtaccttct tctcctaagc ggtcgcccgg gttacgcaac gggcaatcac
     4561 tgcgcgaaag gcagccacaa ccaatacatc cgtccagttc gtcacgcagc gccactaagg
     4621 tatgaatgcg ccgatccaac tcttctcgcc attgggacga aagctgtttc cactctttcg
     4681 cacttaacgt atgcccttcg ggcaacacgc caaacgcttc accaatggtc gccagcggaa
     4741 ttccaatacg ctgagcaatt ttgataattg caacatatcg caacacatca cgtttatatc
     4801 gccgctgatt gccgctgtta cggatactgg taatcaaccc tttactttca tagaaatgca
     4861 gcgccgatac cgccacaccg ctgcgtttcg ccacttcgcc gggggttagc agcgctttaa
     4921 tgcggggtaa tttcttttcc ataaatcgct ttacctcaag ttaacttgag gaattatact
     4981 ccccaacaga tgaattaacg aactgaacac tgaaaagagg cagatttatg tcccatcaga
     5041 aaattattca ggatcttatc gcatggattg acgagcatat tgaccagccg cttaacattg
     5101 atgtagtcgc aaaaaaatca ggctattcaa agtggtactt gcaacgaatg ttccgcacgg
     5161 tgacgcatca gacgcttggc gattacattc gccaacgccg cctgttactg gccgccgttg
     5221 agttgcgcac caccgagcgt ccgatttttg atatcgcaat ggacctgggt tatgtctcgc
     5281 agcagacctt ctcccgcgtt ttccgtcggc agtttgatcg cactcccagc gattatcgcc
     5341 accgcctgta attttattgc ccgcgcgtta actcccgcgc gggtaattgc tccatccatt
     5401 gcataaacac ctgcggcggc atcgccttcg caaagaacca tccctggcaa tagcgcacgc
     5461 cgcgtttgcg cagccagtta acctgctcct cagtttcgac gccttcagcg atcgttttta
     5521 accccaggct gtgcgccagc tcgatgatgt gttccgcaat caaatgactg gttttgtggg
     5581 tggtcagcgt ttcaacaaac gatttgtcga ttttcaaaat atcgacattc aatgatttaa
     5641 ggttatgcaa gttagagtag ccaataccaa aatcatcaat tgccacttcg taacctgcct
     5701 ggcggaaagc cagaataatc ggcgtcattt tgtcaacatc aagaaatgca tgttcagtca
     5761 cttcaaattt aatttgctgc ggacgcaccg cgtattgctc tgttttctga ttgattcgcg
     5821 ctatcaaccg tgacgtatga aaatcggagg ccgacaggtt aatagaaaca tagcgatctg
     5881 catgtgttgc caggtaatcg cccagatcgc ggaagacatt atcaataaca taatcagtta
     5941 cctgttctat catcccctcc ttttctgcca gcggaataaa ctctgccgga ttcattattt
     6001 gcccctgctc accaagccaa cgtaacaacg cttcagcgcc gatacatttt tctgttttga
     6061 tatcgattat tggctggtaa taaagacaaa gttgatgttt ttcgagggcg cgttgcaatt
     6121 tacgtttggg agacaaatag ttttgtcgaa tacgtagcca gagtaatagc agaacaagac
     6181 tccccaaaat accggcgggc aacgcaaaaa taagatgatt ataaaaatgg gttataagac
     6241 gttgatatga agtcgcaaca atggctgcaa ttgggcgttt tgtcgaataa actgtcgcat
     6301 ataaatagcc atttctttgt acggttaaat ccttcaaatg aatcagcgga gaaaacgttg
     6361 ctgccgaggc ctctttgctt aacgagaaaa aggttttcgt caccgtatca tacacacccc
     6421 attgcaatgt cgggtcatca gacatcactt cactccagaa gagagggttg ataaccgcca
     6481 cataatttcc ccgctgcata taggtcattt tatagccaga gaaaaaaggc gtatcgcggt
     6541 aataatagat agaaacgtta ggttcacgct tataatcggc cggtgcaatc gtatagccgt
     6601 ttacaggcgc tatcagcgat gagcataaaa aatggttatc acgggcatag atcaattcat
     6661 taatataaag atagccacga ataatattca acattcgctt ttgatgggct ggagtgcaaa
     6721 cttgcccctg atagcgttca gctgcatcgc tcactaaatc tgcctggcga atgaccagct
     6781 cggatttatc cagtgccagt tgagcaaagg ttcggagctg attattcact tctgattttg
     6841 cccaaaggaa ggcaatccat agcgaaagaa tgatgggaaa gagaactaaa aagataatgc
     6901 ccggcaacgc cagtaattgg tgtcgtgcac gatgactcat tgctatcaat ttcctgcctg
     6961 tttatacccg gccattgcaa ggtgaaaacc ctgggtaccg acgtctttag ataaatctta
     7021 cggtataaat agcacttttg cctgatttga aattgatacc tttctaatcg tcacctggat
     7081 gaaaataatt cattgatatt caaaattaat ttcctacagg aatgcagagg cggcgggaag
     7141 atatgccctt aatgccactg aaaaaccaca taatctgtac agcaataaaa gtcacggcct
     7201 aatatggcta acaaacaatc aattgtgata tagttcacaa aattaatgaa acaaacagag
     7261 tgtttcattt ttgtgttctg caagccccgc gatagccaga gtgtaccacg cctcccgtga
     7321 acaacgccgc gctgtccagg gcatcggctc ttttcagagg aattgtttga tggctaccct
     7381 caccactggc gtggttcttc ttcgctggca acttcttagt gccgtaatga tgtttctggc
     7441 cagcacactc aacatccgtt ttcgtcggtc tgattatgtc gggcttgcgg tgatcagcag
     7501 cggtctgggc gttgtttccg cctgttggtt cgcaatgggg ttgcttggca tcacaatggc
     7561 ggacatcacc gctatctggc acaacatcga gtcggtgatg atagaagaga tgaatcagac
     7621 accgccacaa tggccaatga ttttgacctg atacgtagaa gcctccataa cggaggcttc
     7681 ttttttatgg ctggcagatg ctttaatcat ccaccttaaa acaatataac ctattgtttt
     7741 aatgacaaat cagaacggaa tgtcatcatc aaagtccatc ggcggctcgt tagacggcgc
     7801 tgccggagcg gactgctgcg ggcgagactg cgcgccgccg ctgaactgat tgccaccctg
     7861 cggctgctga ggctgacccc aaccgccctg cggctgacca ccaccgatat tgccacctgc
     7921 cggagcgcca ccaccctgac gaccacccag catctgcatg gtgccgccaa cgttcaccac
     7981 gacttctgtg gtgtagcgat cctgaccgga ttgatcggtc catttacggg tacgcagctg
     8041 accttcgata taaacctgag aacctttacg cagatattcg ctggccactt ctgccagttt
     8101 gccgaacagc acaacgcggt gccattcagt ctgttctttc atctcgccgg tcgctttatc
     8161 acgccaggat tcggaagtag ccagcgtaat gttggcaact gcgccaccat ttggcatgta
     8221 gcgtacttcc gggtcctgac ccagattacc aacgagaata accttgttta cgcctctgct
     8281 ggccatgttc gtgtctcctg aaaaaaatcg ttctgaataa gtgtaaacgc gcgattgtac
     8341 cattaccaat agcgctttta ctatgttgtg acctcggttc cgggaaacaa acctggccag
     8401 acattgttac acaacactcc gggtaatgca ttccaatact gtatattcat tcaggtcaat
     8461 ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca tgccaccggg caaaaaagcg
     8521 tttaatccgg gaaaggtgaa tggataagat cgaagttcgg ggcgcccgca cccataatct
     8581 caaaaacatc aacctcgtta tcccccgcga caagctcatt gtcgtgaccg ggctttcggg
     8641 ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc gaagggcagc gccgttacgt
     8701 tgaatccctt tccgcctacg cgcggcagtt tctgtcactg atggaaaagc cggacgtcga
     8761 tcatattgag gggctttctc ctgccatctc aattgagcag aaatcgacgt ctcataaccc
     8821 gcgttctacg gtggggacaa tcaccgaaat ccacgactat ttgcgtttgt tgttcgcccg
     8881 cgtcggcgaa ccgcgctgtc cggaccacga cgtcccgctg gcggcgcaaa ccgtcagcca
     8941 gatggtggat aacgtgctgt cgcagccgga aggcaagcgt ctgatgctgc tcgcgccaat
     9001 cattaaagag cgcaaaggcg aacacaccaa aacgctggag aacctggcaa gccagggtta
     9061 catccgtgct cgtattgatg gcgaagtctg cgatctttcc gatccgccga aactggaact
     9121 gcaaaagaaa cataccattg aagtggtggt tgatcgcttc aaggtgcgtg acgatcttac
     9181 ccaacgtctt gcggagtcgt ttgaaaccgc gctggagctt tccggtggta cagcggtagt
     9241 ggcggatatg gacgacccga aagcggaaga gctgctgttc tccgccaact tcgcctgccc
     9301 aatttgcggc tacagtatgc gtgaactgga gccgcgactg ttttcgttta acaacccggc
     9361 aggtgcctgc ccgacctgtg acggccttgg cgtacagcaa tatttcgatc ctgaccgcgt
     9421 gatccaaaac cccgagctgt cactggctgg cggtgcgatc cgtggctggg atcgccgcaa
     9481 cttctattac ttccagatgc tgaaatcgct ggcagatcac tataagttcg acgtcgaagc
     9541 gccgtggggc agcctgagcg cgaacgtgca taaagtggtg ttgtacggtt ctggcaaaga
     9601 aaacattgaa ttcaaataca tgaacgatcg tggcgatacc tccatccgtc gtcatccgtt
     9661 cgaaggcgtg ctgcacaata tggagcgccg ttataaagag acagaatcca gtgcggtacg
     9721 tgaagaatta gccaagttta tcagcaatcg cccatgcgcc agctgcgaag ggacgcgtct
     9781 gcgtcgggaa gcgcgccacg tttatgtcga gaatacgccg ctgcctgcta tctccgacat
     9841 gagcatcggt catgcgatgg aattcttcaa caatctcaaa ctcgctggtc aacgggcgaa
     9901 gattgcggaa aaaatcctta aagagatcgg cgatcgtctg aaattcctcg ttaacgtcgg
     9961 cctgaattac ctgacacttt cccgctcggc agaaacactt tccggcggtg aagcccagcg
    10021 tatccgtctg gcgagccaga ttggtgcggg cctggttggc gttatgtacg tgctggacga
    10081 gccatctatc ggcctgcacc agcgcgataa cgagcgcctg ttgggtacgc ttatccatct
    10141 gcgcgatctc ggtaataccg tgattgtggt ggagcacgac gaagacgcaa ttcgcgccgc
    10201 tgaccatgtg atcgacattg gcccgggcgc aggtgttcac ggcggtgaag tggtcgcaga
    10261 aggtccgctg gaagcgatta tggcggtgcc ggagtcgttg accgggcagt acatgagcgg
    10321 caaacgcaag attgaagtgc cgaagaaacg cgttccggcg aatccggaaa aagtgctgaa
    10381 gctgacaggc gcacgcggca acaacctgaa ggacgtgacg ctgacgctgc cggtgggtct
    10441 gtttacctgc atcaccgggg tttcaggttc cggtaaatcg acgctgatta acgacacact
    10501 gttcccgatt gcccaacgcc agttgaatgg ggcgaccatc gccgaaccag caccgtatcg
    10561 cgatattcag gggctggagc atttcgataa agtgatcgat atcgaccaaa gcccaattgg
    10621 tcgtactcca cgttctaacc cggcgaccta taccggcgtg tttacgcctg tgcgcgaact
    10681 gtttgcgggc gtaccggaat cccgtgcgcg tggttatacg ccaggacgtt tcagctttaa
    10741 cgtccgtggc gggcgctgcg aagcctgtca gggcgacggt gtgatcaaag tagagatgca
    10801 cttcctgccg gatatctacg tgccgtgcga tcagtgtaaa ggtaaacgct ataaccgtga
    10861 aacgctggaa attaagtaca aaggcaaaac catccacgaa gtgctggata tgaccatcga
    10921 agaggcgcgt gagttctttg atgccgtacc tgcactggcg cgtaagctgc aaacgttgat
    10981 ggacgttggc ctgacgtaca ttcgcctggg gcagtccgca accacacttt ctggtggtga
    11041 agcccagcgc gtgaagctgg cgcgtgagct gtcaaaacgc ggcaccgggc agacgctgta
    11101 tattctcgac gagccgacca ccggtctgca cttcgccgat attcagcaac tcctcgacgt
    11161 gctgcataaa ctgcgcgatc agggcaacac cattgtggtg attgagcaca atctcgacgt
    11221 gatcaaaacc gctgactgga ttgtcgacct gggaccagaa ggcggcagtg gcggcggcga
    11281 aatcctcgtc tccggtacgc cagaaaccgt cgcggagtgc gaagcatcac acacggcacg
    11341 cttcctcaag ccgatgctgt aatcgttaag gccgctttct gagcggcctt ttcctttcag
    11401 agttgtacca gcaatttacg cttttcttcc ggtagtaaat tcactgcctg ctgataagac
    11461 gcatccacca gataatagat ttgcgaatcc ggcagcgaac cgtcgagata cacggtgctc
    11521 cagtgcgctt tattcagatg gcggctagga cgcacatcgc tgtgctgctg acgtagcagc
    11581 tccgccagct ccgggctggt tttcagcgaa acagctgggc gattttctac ttctttcacc
    11641 atcgcaaaca gtacatcttc cactttgatc tgcgtcgctt tccagtcgtt atgcacgctc
    11701 tgttctgcgc ctggttttgc catgcaatat tgtagcaact ccgaaatggt cattttttac
    11761 tccccttgta gtgtcgcgat gatgcgacgc gatccgccgt ggatgcgatg ttcccccagc
    11821 cagatgcctt gccaggtgcc ggtctgaata cgccctttat gtaccggcaa tacaagcgat
    11881 gttcccagca ttgaggattt gatatgagaa ggcatatcgt ctgctccctc atagtcatgc
    11941 tcataatttc cgttgtcggg aacggtgcgg aggaaaaaac gctccatgtc gtggcgtacg
    12001 gtgggatcgc agttctcatt aagtgtcaga gaggcggagg tatgttgcag caacagatgc
    12061 agtaagccga tgttaacgcg cggcatatca gccagctgat tcagaatttc atccgttacc
    12121 agatgaaacc cacgagattt ggcgctaagc gtgagcgtct tttgatacca catgtgctgc
    12181 tccttgataa aactctctta atcagtttgc agcaagacag cgaaaggata aaggtgtgat
    12241 taaaaaaaca gcattcggga gagcgtcacg ctctcccgct ctgtcagtat tctgaattga
    12301 cgatcacctc ttcaccaaac gcacccgctt gtggcaaggg tttgtaggta gagttggagg
    12361 cgcgcagaat gcggatacca cgagcgccga catcgcgtgc ggcggtaata tcattatcag
    12421 aatcgccata aaaaattcgg atatttttat cctgcagcca ttgcgatttt gtattttgcc
    12481 ctggtttatc gcccgcaaag atcaccggat tcatgttggt ggcaggaata tgaaaattat
    12541 ccgccagcgt ttttgaaacc gtttctgttt tcgtcggact acgaccagtc acaaagaaga
    12601 tcgcgtcacc gcggcgtaca tgcatatcaa tcagctggcg agcgacctct tttggaatgc
    12661 tgaattcatc ccagccattg ttcatttttt cccagaacac aggatttttc agataatctt
    12721 cgctttctgg cgagaaggtt tttttgccgc gccagaagcc cggactggaa aaaagtaccg
    12781 tgtcatcgat atcaaacccc accgccattg gcggacgccc tgcgaggcta ttttcaattt
    12841 gtgcgaccga aacccaatga atgggtgcct gttcagcaag cctggcaacg ttagtcccag
    12901 ggttaagcgg tgaaggagat gaggccaggg caacagcgga actgtttaga gcaaacaata
    12961 agcaaacggc actgattgcc tgtgtgatct tgcgcatatt tttccctaaa tagtcagtat
    13021 gttgaaactt tttattgtga gatttgttgc aaaaaccatc tgaccataac gccagctgcc
    13081 ggggaaagga aggattatct gcggtttttg tgatgaaaag caggatgatt atatttaacc
    13141 cagta
//
LOCUS       DAPGWD010000114        13089 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPGWD010000114 DAPGWD010000000
VERSION     DAPGWD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.115"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..153
                     /locus_tag="RHX88_003214"
     CDS             <1..153
                     /locus_tag="RHX88_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDV2231876.1"
                     /translation="KVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGG
                     CGPTAQ"
     gene            398..1786
                     /gene="frlA"
                     /locus_tag="RHX88_003215"
     CDS             398..1786
                     /gene="frlA"
                     /locus_tag="RHX88_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HDV2231877.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            1807..2829
                     /gene="frlB"
                     /locus_tag="RHX88_003216"
     CDS             1807..2829
                     /gene="frlB"
                     /locus_tag="RHX88_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HDV2231878.1"
                     /translation="MLDIDKSTVDFLVTENMIQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2879..3709
                     /gene="frlC"
                     /locus_tag="RHX88_003217"
     CDS             2879..3709
                     /gene="frlC"
                     /locus_tag="RHX88_003217"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HDV2231879.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYKSNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3706..4491
                     /gene="frlD"
                     /locus_tag="RHX88_003218"
     CDS             3706..4491
                     /gene="frlD"
                     /locus_tag="RHX88_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDV2231880.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4591..5322
                     /gene="phoB"
                     /locus_tag="RHX88_003219"
     CDS             4591..5322
                     /gene="phoB"
                     /locus_tag="RHX88_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HDV2231881.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5474..6559)
                     /locus_tag="RHX88_003220"
     CDS             complement(5474..6559)
                     /locus_tag="RHX88_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV2231882.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6571..7875)
                     /locus_tag="RHX88_003221"
     CDS             complement(6571..7875)
                     /locus_tag="RHX88_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDV2231883.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7887..8240)
                     /locus_tag="RHX88_003222"
     CDS             complement(7887..8240)
                     /locus_tag="RHX88_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDV2231884.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8251..9129)
                     /locus_tag="RHX88_003223"
     CDS             complement(8251..9129)
                     /locus_tag="RHX88_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDV2231885.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9126..10352)
                     /locus_tag="RHX88_003224"
     CDS             complement(9126..10352)
                     /locus_tag="RHX88_003224"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV2231886.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10352..11515)
                     /locus_tag="RHX88_003225"
     CDS             complement(10352..11515)
                     /locus_tag="RHX88_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDV2231887.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHVVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(11599..11961)
                     /locus_tag="RHX88_003226"
     CDS             complement(11599..11961)
                     /locus_tag="RHX88_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDV2231888.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11978..12883)
                     /locus_tag="RHX88_003227"
     CDS             complement(11978..12883)
                     /locus_tag="RHX88_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231889.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
BASE COUNT         3101 a         3691 c         3272 g         3025 t
ORIGIN      
        1 aaagttgcac tggtaggtgc agtactggct acgttaactg catgtactgg tcatattgaa
       61 aaccgtgata agaactgctc ttacgactac ctgctgcacc cggcaatttc tatttctaaa
      121 atcattggcg gttgcggtcc tactgcacag taagtctctg acagaaatcg gctaacaccg
      181 gtttaattgc ccgacgccct ctggattttc cgagggcgta tttgtttcta tatccttctt
      241 gctccccacc cgtaaaaata ttttcacttt aatttcaatt tgataaccac attcattagc
      301 cacagcgcga tcctatgcga aattttgtga tcttcctccg cattacataa catcatatgt
      361 tgttatattc attatgcatt gtcatgttac cttttaaatg actgcaaact ctcccctaca
      421 acgtgttgga caagaaaaag gtatcgctat gggaagccaa gaactccaac gcaagctcgg
      481 attttgggcc gttcttgcaa tcgccgtcgg gacaaccgtc ggctccggta tttttgtatc
      541 tgtgggtgaa gtggcaaaag cagcgggcac gccgtggctt acggtgctcg cgtttgtcat
      601 tggcgggtta attgtgatcc cgcaaatgtg cgtctatgcg gaactatcca ccgcttatcc
      661 ggaaaatggc gcagattatg tttatctgaa aaatgccgga agccgaccgc tggctttcct
      721 ctccggctgg gccagcttct gggccaacga tgcgccgtca ttgtcgatta tggcgctggc
      781 gattgtcagc aatcttggct ttttaacgcc tatcgatccg ttgctcggta aatttatcgc
      841 cgccggatta attatcgcct ttatgttgct acacctgcgc tccgttgaag gcggcgcagc
      901 gtttcagacg ctaattacca tcgccaaaat tatcccgttc actatcgtca ttggccttgg
      961 gatcttctgg tttaaagcgg agaattttgc cgcccctacc accactgcga ttggcgcaac
     1021 gggcagcttt atggcgctgc tggcggggat ctctgccacc agttggtcgt ataccggcat
     1081 ggcctctatc tgttatatga ccggcgaaat taaaaacccc ggaaaaacca tgccacgagc
     1141 gctgattggt tcctgtctgc tggttctggt gctctacacc ctgctggcgc tggtgatttc
     1201 cggcctgatg cccttcgaca aactcgccaa ttctgaaacg ccgatttccg acgccctgac
     1261 ctggatcccc gcactcggca gcaccgctgg gatctttgtt gccatcacgg cgatgatcgt
     1321 cattcttggt tcgctttcca gctgcgtgat gtaccagccg cggctggaat acgcgatggc
     1381 gaaagacaac ctgttcttta aatgcttcgg ccatgtgcat ccgaaataca acacgccgga
     1441 tgtctccatc atcctgcaag gggcgctggg gatcttcttc atcttcgttt ccgatctcac
     1501 cagcctgctg ggttatttca ccctggtgat gtgtttcaaa aataccctca ccttcggctc
     1561 catcatctgg tgtcgtaaac gcgacgatta caaaccgctg tggcgtactc cggctttcgg
     1621 gctgatgacc accctcgcca ttgcgtcaag cctcattctg gtcgcctcaa cctttgtctg
     1681 ggcaccgatt cccggcctta tctgcgccgt catcgttatt gctactggtc tgcctgctta
     1741 cgccttctgg gcgaagcgta gccgccagct caacgctttg tcgtaatgtt atctggagaa
     1801 aataaaatgt tggatattga taaaagcacc gtggactttc tggtcaccga aaatatgatt
     1861 caggaagtgg aaaaagttct cagccatgac gttccgctgg tgcacgccat cgtggaagag
     1921 atggtgaagc gcgacattga tcgtatttat ttcgttgcct gcggctcgcc actcaacgcg
     1981 gcgcaaacgg cgaaacatct ggcggatcgc ttttccgatc ttcaggtcta cgccatttcc
     2041 ggctgggagt tctgcgataa caccccgtat cgcctcgacg atcgttgcgc ggtgattggc
     2101 gtttctgact acggtaaaac ggaagaggta atcaaagcgc tggagctggg ccgggcctgc
     2161 ggcgcactca ctgcggcgtt caccaaacgc gcggacagcc cgattacctc ggcggcggaa
     2221 tttagcattg attatcaggc cgactgtatc tgggaaattc acctgctgct ctgctacagc
     2281 gtggtgctgg agatgatcac ccgcctcgcg ccgaacgcgg aaatcggcaa gatcaaaaac
     2341 gatctcaagc agttgccgaa tgcgctcggt catctggtac gcacctggga agaaaaaggc
     2401 cgccagcttg gtgaactggc cagccagtgg ccgatgattt ataccgttgc tgcgggtccg
     2461 ctgcgtccgc tgggttacaa agaaggcatt gtaacgctga tggaatttac ctggacgcac
     2521 ggctgcgtga ttgagagcgg agagttccgc catggcccgc tggagattgt cgaaccgggc
     2581 gttccgttcc tgttcctgct cggcaatgat gaaagtcgcc acaccaccga acgcgccatt
     2641 aactttgtta aacagcgtac tgacaacgtg atcgtcatcg attacgccga aatttcgcaa
     2701 gggctgcacc cgtggctggc accgttcctg atgttcgtgc caatggagtg gctctgctac
     2761 tacctgtcta tttacaaaga tcacaacccg gatgaacgcc gctattacgg tggtctggtg
     2821 gaatattaat ccctctcccg gcccggcaac gggccgaatt tacgcaagga gttacccgat
     2881 gaaaacaggt atgtttacct gcggccacca gcggctgcct attgaacacg catttcgtga
     2941 tgcaagcgag ctgggttacg acggcatcga aatttggggc ggtcgcccgc acgcgttcgc
     3001 gccggactta aaagcgggcg gcatcaaaca aatcaaggcg ctggcgcaga cgtatcagat
     3061 gccgattatc ggctatacgc cagaaaccaa cggctatccg tataacatga tgctgggcga
     3121 tgaacatatg cgtcgcgaaa gcctcgacat gatcaagctg gcgatggata tggcaaaaga
     3181 gatgaacgcg ggttatacgc tgatttccgc ggcccacgcg ggctatctca cgccacctaa
     3241 tgttatctgg ggacggctgg cagagaacct aagcgaactg tgtgagtacg cggaaaacat
     3301 cggcatggat ctaatcctcg aaccgttaac gccgtataaa tcgaacgtcg tgtgtaatgc
     3361 taatgatgtg cttcatgcgc tggcgctggt gccttcgccg cgcctgttca gcatggtcga
     3421 catttgcgcg ccgtatgtcc aggcggaacc ggtgatgagt tatttcgaca aactgggcga
     3481 taaattacgt catctgcata ttgtcgacag cgacggggcc agcgacacgc attacattcc
     3541 tggagaaggc aaaatgccgc tgagggaact gatgcgcgat attatcgacc ggggctatga
     3601 gggttactgt acggtggagc tggtgacgat gtatatgaac gagcccagac tctatgcccg
     3661 ccaggcgctg gaacgctttc gcgcgctgct gccggaggat gagagatgaa aaccctggcg
     3721 acaatcggcg ataactgcgt cgatatctac ccgcaactga ataaagcgtt ttctggcggt
     3781 aatgcggtca atgtggcggt gtactgcact cgctacggca tacagccggg atgcattacc
     3841 tgggtgggtg acgatgacta cggcacaaag ctgaagcagg atctcgcccg catgggcgtc
     3901 gatatcagcc atgtccatac gaaacacggc gttaccgcac aaactcaggt ggaactgcac
     3961 gacaatgatc gcgtttttgg cgactacacc gaaggcgtga tggccgactt tgccctgagt
     4021 gaagaggatt acgcctggct ggcgcagtat gacattgtgc acgcggcaat ctggggacat
     4081 gcggaagacg cattcccaca gctgcacgct gcgggcaaac ttaccgcttt cgacttctcc
     4141 gacaagtggg acagctcgct ctggcagaca ctggtgccgc atctcgattt tgcctttgcc
     4201 tccgcaccgc aagaagacga agcgctgcgt ctgaagatga aagcgattgt tgcccgtggc
     4261 gcaggcgtgg tgattgtcac gctgggtgaa aacggcagca ttgcctggga tggcgcgcag
     4321 ttctggcgtc aggctcctga accggtgacg gttatcgaca ccatgggtgc cggagattcg
     4381 ttcattgccg gattcctctg cggctggtct gcggggatga cattaccgca ggcgatggcg
     4441 cagggaacgg cgtgcgcggc gaaaaccatt cagtaccacg gtgcctggta ggtataacgt
     4501 tggcgtgagc atcttcacgc caacgtgctg ttacttgccg gaaaacgacc ctataatccg
     4561 agtaattcat tctttatttc agggtcgatt atgtcagcta cggaccgcta ctctcatcaa
     4621 ctcctctacg ctaccgtccg ccagcggttg ctggatgata tcgcgcaggg ggtttaccag
     4681 gccgggcaac agatccctac cgaaaacgag ctttgtacgc aatataacgt cagccgcatt
     4741 actattcgca aagctatcag cgacttagtg gcagacggcg tactgatccg ctggcagggc
     4801 aaaggcacct ttgtacaaag ccagaaggtc gaaaacgccc tgctgaccgt cagtggtttt
     4861 accgattttg gcgtctcaca aggcaaggcg acgaaagaga aagtgatcga gcaggaacga
     4921 gtcagcgccg cgccgttttg cgaaaagctg aacatccctg gcaacagcga agtgttccat
     4981 ctttgccggg tgatgtatct cgataaagag ccgctgttta ttgatagttc ctggatccca
     5041 ctgtcgcgtt atcctgactt tgatgagatt tacgttgaag gaagctccac ttatcagtta
     5101 tttcaggagc gtttcgacac gcgagtggtc agcgacaaaa agaccatcga tatctttgcc
     5161 gccacccgcc cgcaggcaaa atggctgaaa tgcgaactgg gcgaaccgtt gtttcgcatc
     5221 agcaaaatcg cctttgatca gaatgacaaa ccggtgcacg tctccgaact cttctgccgc
     5281 gccaatcgca tcaccttaac gattgataat aaaagacatt aaccgtaggc cggataagat
     5341 gcgccagcat cgcatccggc gatgctggcg cgttgaattt tacatcccgt acgttcccct
     5401 caccctaacc ctctccccaa aggggcgagg ggaccgttca gtacggaatt tagcttgacg
     5461 gtgctatcca gcattaaata ctggcaatac tctcacgcaa aatccgcagc accgtctctt
     5521 caccgctgcg attcgggtta atgcgaatcg cacaatgttc tgattgtgga ttcgcctggc
     5581 gaaacgttcc ggaaaggcga taaaagagcg gcgggatttc atatttcgac tctgcaccca
     5641 ccgggtaagg caaggcaccg cgcttttgcg cctcttccag cactctggcg gcaatcggct
     5701 gatgaaactc gacaatcaac accttcgact gcgcattagc aatcaccgcg cttttcactt
     5761 ccggcaccgc accaccgtta agcaaagcca gcaaccgttc agatacccct gcctgcaccg
     5821 cgtgcatcac tggcgcaaac accagaccac gcaatacttc cagcgcctgt gcgccctgga
     5881 tctggctacc gccggagtaa agcgtggcgc gaatacggtt gataacatca gcatcgccga
     5941 ccactgcacc aacaccctct ggcccaaata gcttgaagca ggaaaatgtc gagacattcg
     6001 cgccgcattc acagccgatt cgcgccacct tcatcaccgc atagttgtca tcggttaacg
     6061 ctggaacacc tgccgcgcgc aacgttgcca gcacatctgc cagcacgtag ctgtcctgcg
     6121 gctgctggcg cgtatgctgc accagcgccg catccggttg ttgctcgtcg acgacctgct
     6181 tcagtgccga caggtcattg aaatcaacag taataagcgt cagccccatc tgctcaataa
     6241 taacccgtgt cgtcgggtaa acaggcgcgt catgcaccag aagacgctgc cccggtttga
     6301 gcaaagccgc cagcccggcg cgaatcgcgc cagtccccgc gccctgcacc agcgccgcag
     6361 cctgtgcgtg aaatgcatca gccagcacct gctccacccg ttgggtaata cgcggttgat
     6421 tcagacctgg agttaaccct aaatcaccgc cagtaagaaa ctcgctgccg gggaaatggc
     6481 gacaaatgct atccaccagc gcaaactgct tttgctgcgc ctcaataatc gtcaggcttt
     6541 gcagaggaaa cgtcttcata ggtgccttcc ttaagccgga acgaacaggc caagccagta
     6601 aagaacattc agcagaatac cggtgatcat tactgccacg accggtgccg ccattttctg
     6661 taccggacgg cccagggatt cgttgaggaa gtaaatggca accgcgatag agaatccggt
     6721 ataacccgcc atcttaattg ctgcgaaaat cgaaccgacc agcagcgcca cttccatcag
     6781 catattcatg gcgttacgga tgttatccga cgcattacgc accgacgggt agcgtcccag
     6841 ccatttgccg atcgaacgaa gcagcaagac ttccgccgaa ataaccactg cgcctaatac
     6901 cgccgcaacc atcggatttg gcgagagata gcccaccgca taaacaaagg taaagcccgc
     6961 aactgcatac acgccagtgg ctaacgcggt ggtggcaatc aacggcacaa aacccagtcc
     7021 gcgcataaat tctgccagag ccgcctgatt aatcagcgtt tgcgattgtt ccggcgtcac
     7081 gcctgcggaa tacgctttct ccagtgtgaa gatcgaaact tcgctaccag caaaaatctt
     7141 catactggca acggcggcaa tcaatgctcc cacgatggcg atataaggta agtttttgat
     7201 aatccgcgac gtgcgctctt caaacaccga aagcccgctg gcatcgatgt cattttcatc
     7261 acgatgacgc aggtcgtgag ttatcgcgat ccccagcagc atcaccatgc caataaagat
     7321 ttcgatggat tcagggttaa gatgtgggaa atagcgcacg actaccacac gggtcatcag
     7381 aacaaccacg gcggcgacca gactttgctt ccagccaaac tggtagaaaa tcgccaccag
     7441 cgggaacagt gcaaaagcag aaaccaccgg cgagcttaat tccccgaggc tacctaatac
     7501 atccaccggc agcgcggtca gcagctggtt tactggcaac aggcaagtaa ggatcaacac
     7561 gccccagata gcgccaagac caaacgccat caggctgttt atcgccagta cgccgaggat
     7621 atcggtagga agaaagagta accatgcgtt gagcagcccg gttttcaggg taaaagagat
     7681 ccccaccgag gccacaaaac caatgctcag accaaaagcg atactccccg cctcgcgacg
     7741 attcatatag ccttcaatca gttgcggcag gatcgggcgg atgccgtcat gaaaaacagc
     7801 cgccgagcga tgcgccagaa gcgatgtcat acccgtcagg cacgccacca cgataatctg
     7861 aatatacaga tccatagtcg tgccttttat ttcagatggt taatcagcat cggaatcgcg
     7921 tgttcgacgt gctcaacgga aaggccaaac gccactttcc cttcagcgat cattttggcg
     7981 atatgctcgt ctttcgcttt aatgcctggt ttggcaatgg tgcaactttt gttatagcca
     8041 atcaccgcga tggcaatcga caatgccgcg cccgcaccgg tattacacgc accgatgtag
     8101 taatccagtt gcccggattt cactttcatt gccgcttcca tgtcgttgtg aatgaaaact
     8161 tcaaaactgc caggagccgt cgcttcaatg gtttttttaa tctgctcacg ctgtaagcct
     8221 gcaacgccaa tctttttcat gagtctgtcc ttattggaaa aattgagagg gattttcacg
     8281 taacatcaca tccacatcgg cctgactgaa tcctgactgg cgcaattgcg gaataaaggt
     8341 ggtcaataaa tagtcatagc cataaccacc attggctttt aaatgggagc ggcgcgtaat
     8401 atccatcgac agcatgacgc ggttcagcaa cccacggtca cgtagcgcgt ggagcatcgc
     8461 aatgcgcttt tcgtccgggt agtaactgtt cttaccaatg gtgtcgaact gcacgtacgc
     8521 gccgagatcg atcatcttca aaatgttgtc gagattgtct ttcagatcgc agtgaccaac
     8581 ggtgacgcgc gaaagatcaa ctccgtgggc ttgcaacaga gccagttgtt ccagccccat
     8641 ggtgctgaac gaagtatgcg tggagatcgg gcgtccggtc tggttatgcg ccagcgcagc
     8701 ggcaataaac accttctctt ccagcggcgt aatcttccct tcgctagagc cgatctccgc
     8761 gataatcccg gctttcaggt cggtgccatc gataccctgt tcaatttcat cgaccatctc
     8821 ctgcgccagt tcctgcacgc tgcgggtcgc cacatgttcc gggaaaaacg cgtcctggta
     8881 ataaccggta caggccacca cgttgatccc cgtctcgcgc attacatcaa gcataaattg
     8941 cgcattgcgc cccatgtaac ggttggtcat ctcaatcaca ttacgcacgc cccgggtcat
     9001 caggtcgttc atctcctggc aaatgaacgc atactgatca aggcggcagt ccacgttgtt
     9061 tttaaagccg gagagatcaa tatgcagatg ctcatgggcg agggtgtaac ccgtcggatc
     9121 aaaactcata gggtgtctcc tgcaaaccgg agggaggaaa gaaaagagcg accattttgt
     9181 ggcggtggcg cgcggaaaaa ttcacacacg gtagccccca catcagaaag cgtggtgcgc
     9241 acaccgagct gcgtagcgat catcccttgc tgataaacca gcactggcac cacttcgcgg
     9301 gtatggtggc tgtgaccaat ggtcggatcg ttaccgtgat ccgccatcac gaccaggcaa
     9361 tcatctggct gcatcgcctc aacaagccgg gcaaggttac ggtcaacgac ctgcaaacgt
     9421 tcggcataac gtgcgacgtc ttctgcatga ccagcgaggt cggtttcctg gatattgatg
     9481 caaataaacg ccgtcggatg cgtgttaaat tcgtcgaggg tgatatccat aatccgctgg
     9541 ctatccacca gattttgcca gctcacgcca taaggattgt tgacgatatc tgccacctta
     9601 cccaccagca cggttggcac gcctgcttca tacagttttt gtggcacctg cactttttca
     9661 tcgacgccat agcccatatg cacgacctgg aaaccgttgt cataagcgcc agaacgcggc
     9721 gcattgatac caataaagcg cccttctttg ctttctgcgg catcgagaat gcgttgactg
     9781 tcggttaaca ggccaccaaa tgtaatgacc cgaccgacct gtacctgctc acgcacgata
     9841 cgaccaattt tgattgcgtc gtcaaaagag atcacagaga gattggcggt aatgttatag
     9901 acctggccta aatccgcctc gagattatcg ccaatcgcaa ccgcctgatt gacccacaga
     9961 aattgcagtt catcgccacg gcgctccacc tgccaaccgg cggatactaa tgcctgctca
    10021 acacggtcaa tcacatcgca aaaaggcatc cgcagcggcg gtaacgggcg cgtgcctaaa
    10081 atttcctgat gccccataaa ggtatcgcca ccttcatgtt gcagctctgc cacgccccag
    10141 gttgcggaat ctgacggctg catatcgcct ggcgcataac ccaatgcgtt gattagcccc
    10201 agcttctcca gcgttggtag ctgcaaatgc ggcaactggc tcaggatgtg accacaggta
    10261 ttcgctcccg catcttgcgg acgcaccagc gtgacatctt tcattgcccc tacgccaaag
    10321 ctatcaatca ctaacaccac aaatcgcgcc attacgcccc cagagagttt ccaagactgt
    10381 cataacgacc aacgattttc ggttcgccac ggtgaatacc ggacaccagc accacatcgc
    10441 tacgggtgac aaaaatctgc gtgcggaaac agagcaccac tgcactgctc accggatact
    10501 cgcctgccag cggcagggtg tagtcgatac tgctgtcatc cacagttttg agattggttt
    10561 cagtaatctt ttgattttct ggcgtaaaca ccagcgcatg ttgcgcatga ccacgacggt
    10621 agtaaccgcc gccgtagcag tagctgtcgc cacggaaatg atgggagatt tcacttaacc
    10681 agagcatcgc gatacgttca ggctgatcgc cctgctggtt tgccggaata gtgcccgtca
    10741 gcgcatgacc gggttcggca tgagtcacac cgtattccgc cagcaatggc agcgaagtgc
    10801 agctggtcgc tgaaggcgcg ttcagttgct caagtgcaat accagatttc gccagttgat
    10861 cccgtgcctg tatcagcgtg tgaagattcg gtgtcggcaa aacttttccg gcagcctcat
    10921 cccaaagcag gcaagggaaa tgggtaagtc cggctaaatg cagccccggc agtttcttaa
    10981 tttcagcaac caccacgtgc agcgaatgct gtacaaaacc gctctcctgc cccggataaa
    11041 gaaaatcatc gtcgctataa actttgagca gcacaaactg cacacgtccg gccttcaccg
    11101 ctgccgcaga aacttcccgc gctttgtcga gagtaaacac ggtgatgacg tcggtgccct
    11161 gttcaacggc gtcagcaacc tgatgacaag ggatttgtac cagatgcccc tgatgcgcca
    11221 ccgacaaacc agcgcggcgc atgactcgcg cctctttgta atccaccgcc acaatgccgc
    11281 tgtaacctaa tgccagcaat ttttccgcca gccatgggtt acggccaaac tgcttggtca
    11341 tcagatacag ttcaatgccg taaagccgcg ccgtctcaat cagtcgctta ccgttttcca
    11401 gtacctgatc cacgtcgatc acccagctgt ccggggcgat cttgccctgc tgccacaaac
    11461 ttagtgcggc ggaaatcagc gccgggttct ggcgtttcaa tgcttctaca aacatcttgt
    11521 tgcctcgttt ctgttccttg aattaaatat tcattttttt gaatatttag gttgtgcgac
    11581 gtggatttgt cgggagcttc aaacctcttc gttagcagcc atccataatc cataaaggtt
    11641 ggccagcaaa tagccttctt cacacgggtt aacttccagt gcgaattcct tcaacaacac
    11701 ctgatgcaat tgcacaacgg cttgccagtg gctggattgc gccagctctg ccagcaattc
    11761 gttatccagc ggctctattt cttcaccacg gcgactgcgc atcagtgcac tcgccatatg
    11821 tgtcatcgcc atcgttcctt gctcactgcg caccggcaga tggcactctg tttccagtac
    11881 gttgacgacc tgcatcatgc ctttgcagat gtccttatca ataacgcctg cctcgcaaag
    11941 caggttgagt ctggtttcca tatcagatgc ctttcgttta ataactcggt tcctgttccc
    12001 cactcactac ccgttgttga tgggcgagca gggcgtgttt atctacaacg gcacgcagca
    12061 gttgttgcat tgggtaatca tcgactcgcg tcaggaccac ggcttcggtg gcctgtaaaa
    12121 atcgcggatc gtctgtgagc ggcgtcgcct ctaatcccag catggtcagt tcgttttccg
    12181 ccaccacgtt ccagatcacc gcatcgacat cgcctttaac aatgcgttgt aaactctcgt
    12241 gataagagag atcgactcgt tccacatcac taccgccaaa aaaaacatcg gtcatgattt
    12301 tctgatccgc cgaacggtta tccagcccca cgcgcttcac gtttgcggac tcgcctttac
    12361 ggcaaatcaa ctggtgctcg ccaacgtagg tgtgcggccc caactccagc gcgaggcata
    12421 accctttttg cgtgagataa ctttccgccg ccagtcggga aaccaccgcc atgtcataca
    12481 cgccgttaag taaacactcc acgcgaatat ccgcgccgcg catatgcgca tagtaaaaag
    12541 gaatgccatc aaactgagct ttcagcccgc tcgctaaacc ttcgtacaag cgggtatagg
    12601 gcaaaggcat cgcacatacc acgttgttga tatccacatg actcagcaat gctttgttat
    12661 ccatctcgac cagataactg ccattgcgcc cacggcgttc aatccgtatc gctccgcttg
    12721 attccagcgt tttcaacgcg gcctgcgtca ggccaaccga tgaacggcat tcagttgcca
    12781 gctcatctat ggttttcaac ctattaccgc atttttcacc caacaaataa cgggccagcg
    12841 tcgtgataac gacgccttct tttttgataa acgttcgacg cataatatat tttcaataaa
    12901 atgaatgttt atatcttcat taaaatgaag ataagtggca actgtgagat gtggaaacgg
    12961 cgaggcgctt cacatttttt cggattattg caaatgagga taaaaaaacc gggtttcccc
    13021 ggtttcagag tgatgacgaa gtgatttatt cgacaatgcg agcaaagggt aattcccact
    13081 ggcgcttgc
//
LOCUS       DAPGWD010000115        13012 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPGWD010000115 DAPGWD010000000
VERSION     DAPGWD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.116"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            247..582
                     /locus_tag="RHX88_003228"
     CDS             247..582
                     /locus_tag="RHX88_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDV2231890.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            742..1185
                     /gene="yjdN"
                     /locus_tag="RHX88_003229"
     CDS             742..1185
                     /gene="yjdN"
                     /locus_tag="RHX88_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDV2231891.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1318..2106
                     /gene="phnC"
                     /locus_tag="RHX88_003230"
     CDS             1318..2106
                     /gene="phnC"
                     /locus_tag="RHX88_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDV2231892.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2131..3147
                     /gene="phnD"
                     /locus_tag="RHX88_003231"
     CDS             2131..3147
                     /gene="phnD"
                     /locus_tag="RHX88_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2231893.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3253..4032
                     /gene="phnE"
                     /locus_tag="RHX88_003232"
     CDS             3253..4032
                     /gene="phnE"
                     /locus_tag="RHX88_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDV2231894.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4053..4778
                     /gene="phnF"
                     /locus_tag="RHX88_003233"
     CDS             4053..4778
                     /gene="phnF"
                     /locus_tag="RHX88_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDV2231895.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4779..5231
                     /gene="phnG"
                     /locus_tag="RHX88_003234"
     CDS             4779..5231
                     /gene="phnG"
                     /locus_tag="RHX88_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDV2231896.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5228..5812
                     /gene="phnH"
                     /locus_tag="RHX88_003235"
     CDS             5228..5812
                     /gene="phnH"
                     /locus_tag="RHX88_003235"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDV2231897.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5812..6876
                     /gene="phnI"
                     /locus_tag="RHX88_003236"
     CDS             5812..6876
                     /gene="phnI"
                     /locus_tag="RHX88_003236"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDV2231898.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6869..7714
                     /gene="phnJ"
                     /locus_tag="RHX88_003237"
     CDS             6869..7714
                     /gene="phnJ"
                     /locus_tag="RHX88_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDV2231899.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7711..8469
                     /gene="phnK"
                     /locus_tag="RHX88_003238"
     CDS             7711..8469
                     /gene="phnK"
                     /locus_tag="RHX88_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDV2231900.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8580..9260
                     /gene="phnL"
                     /locus_tag="RHX88_003239"
     CDS             8580..9260
                     /gene="phnL"
                     /locus_tag="RHX88_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDV2231901.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9257..10393
                     /gene="phnM"
                     /locus_tag="RHX88_003240"
     CDS             9257..10393
                     /gene="phnM"
                     /locus_tag="RHX88_003240"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDV2231902.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10393..10950
                     /gene="phnN"
                     /locus_tag="RHX88_003241"
     CDS             10393..10950
                     /gene="phnN"
                     /locus_tag="RHX88_003241"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDV2231903.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10937..11371
                     /gene="phnO"
                     /locus_tag="RHX88_003242"
     CDS             10937..11371
                     /gene="phnO"
                     /locus_tag="RHX88_003242"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDV2231904.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11373..12131
                     /gene="phnP"
                     /locus_tag="RHX88_003243"
     CDS             11373..12131
                     /gene="phnP"
                     /locus_tag="RHX88_003243"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDV2231905.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12278..12607)
                     /gene="yjdP"
                     /locus_tag="RHX88_003244"
     CDS             complement(12278..12607)
                     /gene="yjdP"
                     /locus_tag="RHX88_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDV2231906.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12676..12993)
                     /locus_tag="RHX88_003245"
     CDS             complement(12676..12993)
                     /locus_tag="RHX88_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HDV2231907.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2854 a         3916 c         3658 g         2584 t
ORIGIN      
        1 cgtcagaaag ataaaaatat catgtgaata aaaaaaagaa caagtagagc attaacatta
       61 tcttaaataa taaatagagg caaaaagatt attttctttt tgcgtttcct ttcaaatgaa
      121 aacgatcgtc gtctaaaatc agcagtaccc ccgacaaact cagggatttt gtgtataatt
      181 gcggcctttt tcggcaatct gccgtttttt ggcgcttttg ccctgctgac ttttgaggaa
      241 atccacatgt cattaccaca ctgcccaaaa tgcaactccg aatacactta cgaagataac
      301 ggcatgtaca tctgcccgga atgtgcctac gaatggaacg acgcagaacc tgcacaggaa
      361 agcgacgagc tgatcgttaa agatgctaac ggcaatctgc tggctgacgg cgacagcgtt
      421 accatcatta aagatctgaa ggtgaaaggt agctcttcga tgctgaaaat tggcaccaaa
      481 gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg attgcaaaat cgacggtttt
      541 ggtccgatga aactgaaatc tgagtttgtg aaaaagaact gattgtattg tgatcggtaa
      601 gccggataag gcgctcgcgc cgcatccggc aacgatgccg actgcctgat gcgacgctga
      661 ccgcgtctta tcaggcttcc ctctcatctg tataaatttc gaactacact taactggctt
      721 ctcttaactg aggtcaccat catgccgtta agtccctacc tctcttttgc cggtaactgt
      781 gccgacgcaa ttgcttatta tcaacgtacg ttgggcgctg aactgctcta taaaatcagc
      841 ttcggcgaaa tgccaaaatc agcgcaggac agcgccgaga actgcccttc cggaatgcaa
      901 tttcccgata ccgccatcgc tcatgccaac gtgcgcattg ccggaagcga catcatgatg
      961 agcgatgcca tgccgtcagg aaaagccagc tactccggct ttacgctggt gctcgattcg
     1021 caacaggtcg aagaaggaaa acgctggttt gacaatcttg ccgctaacgg aaaaatcgaa
     1081 atggcctggc aggaaacttt ctgggcgcat ggctttggca aagtcaccga taaatttggc
     1141 gtaccgtgga tgattaatgt cgtcaaacaa caaccaacgc aataatccgc cgggaggccc
     1201 gccctcccgc actgtcatcg aattcccgtt aactcttcat ctgttagtca cttttaatta
     1261 accaaatcgt cacaataatc cgccacgatg gagccacttt tttagggagg ctgcatcatg
     1321 caaacgatta tccgtgtcga gaagctcgcc aaaaccttca atcagcatca ggcgctgcat
     1381 gcggttgatc tgaacattca tcacggtgaa atggtggctc tgcttgggcc gtcgggttcc
     1441 ggcaaatcca cccttttacg tcacttaagc ggtttgatta ccggcgataa atccgccggc
     1501 agccatatcg agctgctggg ccgcacagtc cagcgcgaag gccgtctggc acgcgatatc
     1561 cgcaaaagcc gcgccaacac cggctacatc ttccaacaat tcaacctggt gaaccgcctg
     1621 agcgtactgg agaacgtgct gattggcgcg ctcggcagca cgccgttctg gcgcacctgt
     1681 tttagctggt tcaccggcga gcagaaacag cgcgcgttac aggcgctgac ccgcgttggc
     1741 atggtgcatt ttgcccatca gcgcgtttcc accctctccg gcggacagca gcagcgtgtg
     1801 gcgattgccc gcgcgctgat gcagcaggcg aaggtgattc tggccgatga acccatcgcc
     1861 tcgctggacc cggaatccgc ccgcatcgtg atggacaccc tgcgcgacat caatcagaac
     1921 gacggcatca ccgtggtcgt cacgctgcat caggtggatt acgccctgcg ctactgcgaa
     1981 cgcatcgtcg ccctgcgcca ggggcacgtt ttctacgacg gcagcagcca acagtttgat
     2041 aacgaacgtt ttgaccatct ctaccgcagc attaatcgca tcgaagagaa cgcgaaagct
     2101 gcctgacatc cccatcattg aggaaaacga atgaacgcta agataattgc ctcgctggcc
     2161 ttcaccagca tgttcagcct cagcaccctg ttaagcccgg cacacgccga agagcaggaa
     2221 aaggcgctga atttcggcat tatttcaacg gaatcacagc aaaacctgaa accgcaatgg
     2281 acgccattct tacaggatat ggagaagaag ctgggcgtga aggtgaacgc cttctttgcc
     2341 ccagactacg caggcattat ccagggaatg cgcttcaata aagtggatat cgcctggtac
     2401 ggcaacctgt cggcaatgga agcggtggat cgcgccaacg gccaggtctt cgcccagacg
     2461 gtcgcggcgg atggatcgcc aggttactgg agcgtgttga tcgtcaacaa agatagtccg
     2521 atcaacaacc tgaacgatct gctggcgaag cggaaagatc tcaccttcgg caatggcgat
     2581 cctaactcca cctctggctt cctcgtcccc ggttactacg tctttgccaa aaacaatatc
     2641 tccgccagcg actttaagcg caccgtcaac gctgggcatg aaaccaacgc gctggccgtc
     2701 gccaacaagc aggtggatgt tgccaccaac aacaccgaaa acctcgacaa gctgaaaacc
     2761 tccgcgccag agaagctgaa agaactgaag gtgatctgga agtcgccgct gatcccaggc
     2821 gatccgatcg tctggcgtaa aaacctttcc gaaaccacca aagacaagat ctacgacttc
     2881 tttatgaact acggcaaaac gccggaagaa aaagcggtgc tggaacgcct gggctgggcg
     2941 ccgttccgcg cttccagcga cctgcaactg gtgccgattc gccagctcgc gctgtttaaa
     3001 gagatgcagg gcgtgaaaag caataaagga ctgaatgagc aggacaagct ggcaaaaacc
     3061 accgagattc aggcgcagct ggatgacctg gaccgcctga acaacgcgtt aagcgcgatg
     3121 agttcggtga gtaaagcggt gcagtaaatc gtaggtcgga taagacgccc cggcgtcgca
     3181 tccgacaatg tgcaggcgtt gatgccggat gcggtgcaag caccttatcc ggcctacaga
     3241 ccggagccaa acatgcaaac catcaccatc gccccaccca agcgcagctg gttctcgctt
     3301 ctgagctggg ccgttgttct cgccgtgttg gtcgtctcgt ggcagggcgc ggaaatggcc
     3361 ccgctcacgc tgattaaaga cggcggcaac atggcgacgt tcgccgccga cttcttcccg
     3421 cccgatttca gccagtggca ggattacctc accgaaatgg ccgtcacgct gcaaatcgcc
     3481 gtctggggca cggcgctggc ggtggttctc tccatcccct tcggcctgat gagcgccgaa
     3541 aacctggtgc cgtggtgggt ttaccagccc gttcgccgcc tgatggacgc ctgccgcgcc
     3601 attaacgaaa tggtcttcgc catgctgttc gtggtcgccg tcggcctcgg cccgttcgct
     3661 ggcgtgctgg cgctgtttat ccacaccacc ggcgtgctct ccaaactgct ttccgaagcg
     3721 gtggaagcga ttgaacccgg cccggtggaa ggcattcgcg ccaccggcgc caacaagctc
     3781 gaagagatcc tctacggcgt gctgccgcag gtgatgccgc tgctgatctc ctactccctc
     3841 tatcgcttcg aatccaacgt ccgctcggcg accgtcgtcg gcatggtcgg tgcaggcggg
     3901 atcggcgtca ccctgtggga agcgattcgc ggtttccagt tccaacaaac ctgcgccctg
     3961 atggtgctta tcatcgtcac ggtcagcctg ctggatttcc tctctcaacg gttgcgtaag
     4021 cactttatct gataagcgag gcattgatat ctatgcactt gtctacacat ccgaccagct
     4081 acccaacacg ctatcaggag atagccgcaa aacttgagca ggagcttcgt caacactacc
     4141 gctgcggcga ctatcttccc gccgagcagc aactggcggc gcgctttgag gtgaaccgcc
     4201 acaccctgcg ccgcgccatc gaccaactgg tggaaaaagg ctgggtacag cgccgtcagg
     4261 gcgtcggcgt gctggtgctg atgcgcccgt tcgattaccc gctcaacgcc caggcgcgtt
     4321 ttagccagaa cctgctggac cagggtagcc atcccaccag cgaaaaactg ctgtcggtat
     4381 tgcgccccgc gtccggtcac gtcgctgacg cgctggggat taccgagggc gagaacgtta
     4441 tccatttgcg caccctgcgt cgcgtcaacg gcgtcgcgct ctgtttaatc gaccactact
     4501 ttgcggacct caccctttgg ccgacgctgc aacgcttcga cagcggttcg ctgcacgatt
     4561 ttctgcgcga gcaaaccgga attgcgctgc gccgcagcca gacgcggatc agcgcccgtc
     4621 gcgcccaggc caaagagtgc cagcgtcttg aaattccgaa tatgtcgccg ctgctgtgcg
     4681 tgcgcaccct taaccaccgt gacggtgaaa gcagcccggc ggagtactcc gtcagcctga
     4741 cgcgcgccga catgattgaa ttcactatgg agcactgaat gcacgcagat accgcgaccc
     4801 gccagcactg gatgtccgta ctggcgcaca gccaaccggc tgaactggca gcccgcctga
     4861 aagcactgaa catcaccgcc gactatgagg ttatccgcgc cgccgaaacc ggcctggtgc
     4921 agattcaggc gcggatgggc ggcaccggcg aacgcttttt tgccggcgac gccacgctca
     4981 cccgcgccgc cgtacgcctg actgacggca cgctcggcta cagctgggtg ctggggcgcg
     5041 acaaacagca cgccgaacgc tgcgcgctga ttgacgcgct gatgcagcaa tctcgctatt
     5101 ttcaaaactt atcagaaacc cttattgccc cgctggacgc tgaccgtatg gcacgcattg
     5161 ccgcacgcca ggccgaagtg aacgccagcc gggtcgactt ctttacgatg gttcgcggag
     5221 acaacgcatg accctggaaa ccgcttttat gcttcccgtg caggatgccc agcacagttt
     5281 tcgtcgcctg ttaaaggcca tgagcgagcc gggcgtgatt gtcgccctgc atcagctcaa
     5341 gcgcggctgg cagccgctga atatcgccac caccagcgtg ctgctgacgc tggccgataa
     5401 cgacacgccg gtgtggcttg ccgcgccatt gagtaacgat atcgtcagcc agagcctgcg
     5461 ttttcatacc aacgcgccgc tggtcagcca gccggaacag gcgaccttcg cggtgacgga
     5521 tgaggcgatt tccagcgaac agcttaacgc cctttccacc ggcaccgccg ttgcgccgga
     5581 agcgggtgca acgctgattt tacaggtcgc cagcctgagc ggcgggcgca tgttacgtct
     5641 caccggcgcg ggcattgccg aagaacgaat gatcgctccg cagctgccgg agtgcattct
     5701 gcacgaactc accgagcgcc cgcacccgtt cccgctcggc atcgacctga tcctgacctg
     5761 cggcgagcgc ctgctggcta ttccgcgaac cactcatgtg gaggtgtgct gatgtacgtt
     5821 gccgtgaaag ggggcgagaa ggcgatcgac gccgctcacg ccctgcaaga gagccgacgc
     5881 cggggcgata ccgatttgcc tgaactgagc gtcgcccaga ttgaacagca gcttaatctt
     5941 gcggtggatc gcgtgatgac cgaaggcggc attgccgacc gcgaactggc ggcgctggcg
     6001 ctgaaacagg ccagcggcga taacgtcgaa gcaattttcc tgctgcgcgc ctaccgcacc
     6061 acgctggcga agctcgcggt aagcgagccg ctcgacacca cagagatgcg tctcgaacgg
     6121 cgtatttcgg cggtttataa agacattccc ggcggccagc tgcttggccc aacctacgac
     6181 tacacccatc gcctgctcga tttcaccctg ctggcaaacg gtgaagcgcc gacgctgacc
     6241 accgccgaca gcgaacaaca gccgtcgccg cacgttttca gcctgctggc gcgtcagggg
     6301 ctggcgaagt ttgaagagga tagcggcgca cagccggatg atatcacccg cacgccgccg
     6361 gtttacccct gctcacgttc ttcccgtttg cagcagttga tgcgcggcga cgaaggctat
     6421 ttgctggcgc tggcctactc cacccaacgc ggttacgggc gcaatcaccc gttcgcgggc
     6481 gagatccgca gtggttacat cgacgtgtcg attgtgccgg aagagctggg atttgcggtg
     6541 aacgtcggcg aactgctgat gaccgaatgt gaaatggtca acggttttat cgacccgccg
     6601 gatgagccgc cgcacttcac gcgcggctac gggctggtat tcggcatgag cgagcgcaaa
     6661 gcgatggcaa tggcgctggt cgatcgtgcg ttgcaggctc cggaatacgg cgagcacgcg
     6721 acaggcccgg cgcaggatga agagtttgtg ctggcacatg ccgacaacgt cgaagccgca
     6781 ggctttgtct cgcacctcaa actcccccac tacgtcgatt tccaggccga actggagcta
     6841 ctcaaacgtc tgcaacagga gaagaaccat ggctaatctg agcggctaca actttgccta
     6901 cctcgacgag cagaccaaac gcatgatccg ccgcgctatc ttaaaagcgg tggcgatccc
     6961 cggttatcag gtgccgtttg gcgggcgcga gatgccgatg ccatacggct ggggaaccgg
     7021 cggcatacag ctcaccgcca gcgtgattgg cgaaagcgac gtgctgaagg tgattgacca
     7081 gggtgcggat gacaccacca acgccgtgtc gattcgcaac ttctttaagc gcgtgaccgg
     7141 ggtaaacacc actgaacgta cggacgatgc gacgcttatc cagacgcgtc accgcatccc
     7201 cgaaacgccg ctgaccgaag atcagatcat tatcttccag gtgccaatcc cggaaccgct
     7261 gcgctttatc gagccgcgcg aaacggaaac ccgcaccatg cacgcgctgg aagagtacgg
     7321 cgtgatgcag gtgaaactgt atgaagatat cgcccgcttc ggtcatatcg ccactaccta
     7381 cgcctatccg gtgaaggtga acgggcgcta cgtaatggac ccgtcgccga tcccgaaatt
     7441 cgataaccca aaaatggaca tgatgcccgc cctgcaactg ttcggcgcgg ggcgcgagaa
     7501 gcgcatctat gcggtgccgc cgtttacccg cgtggaaagt ctcgatttcg acgatcaccc
     7561 gttcaccgtt cagcagtggg atgagccatg cgccatctgc ggatcgaccc acagctatct
     7621 tgatgaagtg gtgctggatg acgccggaaa ccgcatgttt gtctgctccg ataccgatta
     7681 ttgccgccaa cagagcgagg caaaaaacca atgaatcaac cgttactttc ggtcaataac
     7741 ctgacccacc tttacgcgcc gggcaaaggc tttagcgatg tctcttttga tttatggccg
     7801 ggggaagtgc tgggcattgt cggggagtcc ggctccggga agaccacgct gctgaagtcg
     7861 atctccgcgc gtctgacgcc gcagcagggg gagattcact acgagaaccg ttcgctgtat
     7921 gcaatgagcg aggccgaccg ccgtcgcctg ctgcgcaccg aatggggcgt agtgcatcag
     7981 catccactcg acggcctgcg ccgtcaggtc tcggcaggcg gcaatatcgg cgagcggttg
     8041 atggcgaccg gggcgcgtca ttacggcgac attcgcgcca ccgcgcagaa gtggctggaa
     8101 gaggtggaga ttcccgccaa ccggatcgac gacctgccga ccaccttttc cggcggtatg
     8161 cagcagcgtt tgcagattgc ccgcaacctg gtgacgcatc cgaagctggt gtttatggat
     8221 gaaccgaccg gcgggctgga tgtgtcggtg caggcccgcc tgctcgacct gctgcgcggc
     8281 ctggtggtgg agctgaacct cgcggtggtg attgtcaccc atgatttagg cgtcgcccgc
     8341 ctgctggcgg accgtttgct ggtgatgaag caggggcaag tggtggagag tgggttaacc
     8401 gaccgcgtgc tcgacgaccc gcatcatccg tatacacagc tgctggtgtc atcggttttg
     8461 cagaattgag ccggtgccgg atgcggcgta aacgccttat ccggcctaca aatgcgctcc
     8521 ccgtaggtcg gataagacgc gtcagcgtcg catccgacac ccgaaccacg aggcgaaaaa
     8581 tgattaacgt acaaaacgtc agtaaaacct tcatcctgca ccagcaaaac ggcgtgcgcc
     8641 tgcccgtctt acaacgcgcc tcgctcaccg tcaacgcagg tgaatgcgtg gtgctccacg
     8701 gccattccgg cagcggcaaa tcaactctgc tacgctcgct gtacgccaac tatctgcccg
     8761 acgaagggca aatccagatc aaacacggtg acgaatgggt agacctggtc accgcgccag
     8821 cgcgcaaagt ggtggagatc cgcaaaacta ccatcggctg ggtgagccag tttctgcgcg
     8881 tcatcccgcg tatttcagca ctggaagtgg tgatgcagcc gctgctcgat accggcgttc
     8941 cgcgtgaagc ctgcgccgct aaagccgcgc gtcttctcac ccgcctgaac gtgccggaac
     9001 gcctgtggca cctggcacca tcgaccttct ccggcggcga acagcagcgc gtcaacatcg
     9061 cccgcggctt tatcgtcgac taccccattc tgctgctcga cgaacctacc gcctcgctgg
     9121 acgccaaaaa cagcgccgcc gtagtcgagc tgattcgcga agccaaaacc cgtggcgcgg
     9181 ccatcgtcgg catcttccat gacgaagccg tacgtaatga cgtcgccgac cgcttgcacc
     9241 caatgggagc ctctgcatga ttatcaataa cgtcaaactg gtgctggaaa acgaggtggt
     9301 gcacggttcg ctggaggtgc aggatggcga aatccgcgcc tttgccgaaa gccagagccg
     9361 cctgccagaa gcgatggacg gcgaaggtgg ctggctacta cccggcctga ttgagctgca
     9421 taccgacaac ctcgataaat tcttcacccc gcgcccgaaa gtcgactggc ccgcccattc
     9481 ggcgatgagc agccacgacg cgctgatggt ggcaagcggc atcaccaccg tgctggacgc
     9541 ggtggcgatt ggcgacgtgc gcgacggcgg cgatcggctg gagaatctgg agaagatgat
     9601 caacaccatc gaagagacgc agaaacgcgg cgtcaaccgc gccgagcacc gcctgcacct
     9661 gcgctgcgaa ctgccgcatc acaccacact gccgctgttt gaaaaactgg ttcagcgcga
     9721 accggtgacg ctggtgtcgc tgatggacca ctcaccgggc cagcgccagt tcgctaaccg
     9781 cgagaagtat cgcgaatatt atcagggcaa atactccctc accgatgcgc agatgcagca
     9841 gtacgaagaa gagcaattgg cgctcgccgc acgctggtcg cagccgaatc gcgaatccat
     9901 cgccgccctg tgccacgcgc gacaaattgc gctcgccagc cacgatgacg ccacgcacgc
     9961 ccacgttgcc gaatcccacc aacttggcag cgtgatcgcc gaatttccca ccacgttcga
    10021 agcggcggaa gcctcgcgta agcatggcat gaacgtgctg atgggtgcgc caaatatcgt
    10081 gcgtggcggc tcgcactccg gcaatgtggc ggccagtgaa ctggcgcagc ttggtctgct
    10141 ggatatcctc tcttccgact actaccccgc cagcctgctg gatgcggcgt tccgcgtcgc
    10201 cgatgacgag agcaaccgct ttacgctacc gcaggcggtg aggctggtga ccaaaaatcc
    10261 ggcgcaggcg ctgaatcttc aggatcgcgg ggtgattggc gagggtaaac gcgctgacct
    10321 ggtgctggcg catcgccagg gcaatcacat tcatatcgac cacgtctggc gtcagggtaa
    10381 aagggtgttc tgatgatggg aaaactgatt tggttaatgg ggccgtccgg ctccgggaaa
    10441 gacagcctgc tggcggaact ccgcctgcgg gaacaaacgc agttactggt ggcgcatcgc
    10501 tacatcacgc gcgacgccag cgccggaagt gaaaaccata tcgccctgag tgagcgggag
    10561 ttttttaccc gcgcggggca aaacctgctg gccttaagct ggcacgctaa cggtctgtat
    10621 tatggcgtcg gcatcgagat tgacctctgg ctgcacgctg gattcgacgt agtcgtcaac
    10681 ggttcacgcg cgcatttgcc gcaggcacgg gcgcgctatc aatcggcgct gctgcccgtc
    10741 tgtttacagg tttcgccgga gatcctgcgc cagcgcctgg aaaaccgtgg ccgtgaaaac
    10801 gccagtgaaa ttaacgcccg cctggcgcgc gccgcccgct atactccaca ggattgccat
    10861 acgctcaaca atgacggcag cctgcgccag tcggtcgaca cgctgctgac gctgatccat
    10921 cagaaggaga aacaccatgc ctgcttgtga gcttcgcccg gccacgcagt acgataccga
    10981 cgcggtttac gcgctgattt gtgagctaaa acaggcggag tttgaccacc atgcgtttcg
    11041 cgtgggcttt aacgccaacc tgcgcgaccc aaacatgcgc taccatctgg cgctgctgga
    11101 tggcgaagtt gtcggcatga tcggcctgca tttgcagttt catctgcatc atgtcaactg
    11161 gatcggcgaa attcaggaac tggtggtgat gccgcaggcg cgcggtctga acgtcggcag
    11221 taaattactg gcatgggcag aagaagaagc ccgccaggcc ggggccgaaa tgaccgagct
    11281 ttccaccaac gtgaagcgcc acgacgcaca ccgtttttac ctgcgtgaag gctacgagca
    11341 gagccacttc cgcttcacca aggcgctgta acatgagcct gaccctcacg ctcaccggca
    11401 ctggcggcgc acagggcgtt ccggcatggg gctgcgagtg cgcggcctgc gccagagcgc
    11461 ggcgctcgcc gcagtatcgc cgccagccat gcagcggcgt agtgaagttt aacgacgcga
    11521 tcaccctgat cgacgccggg cggcacgatc tcaccgatcg ctggtcgcct ggatcgttcc
    11581 agcagttttt gctgacgcac taccatatgg atcacgtcca agggctattt ccgctacgct
    11641 ggggcgtggg cgatgtgatc ccggtctacg gcccgccgga tgaacagggc tgcgacgatc
    11701 tgtttaaaca tccgggcctg cttgatttca gccacacagt ggaaccgttt gtggtgtttg
    11761 atttgcaggg gttgcaggtc acgccgctgc cgcttaacca ctcaaaactg accttcggtt
    11821 atctgctgga aacggcacat agccgggtgg cgtggctgtc tgacaccgct ggcctgccgg
    11881 aaaaaacgct gaaattttta ctcaataatc atccgcaggt gatggtgatt gattgcagtc
    11941 acccgccgcg cgcggatgca ccgcgtaatc actgtgattt aaataccgtg cttgcgctga
    12001 atcaggttat ccgctcgcca caggtgattc tgacccatat cagccaccag tttgatgcgt
    12061 ggctgatgga aaacgcacta ccgtcagggt ttgaggtggg gtttgatggg atggagattg
    12121 gggtggcgtg atgagaggga atgtgcgcgc tggccccctc accctaaccc tctccccaga
    12181 ggggcgaggg gaccgattgt gctcaatatt gaatattgcg ctcgttttct ccctctcccc
    12241 attggggtga ggggcgatgc ctgctccata cccaacctca tcgcccatac tcatcttcca
    12301 ttcttcgctc ttcatcctcc agttgccgac gctcctgatc aagctggcgc tggcgatcgt
    12361 ccagctgcct gcggcgatct tcaaactggc ggcggcggtc gtcatattgt ctgcgccgat
    12421 cgtcgctcac ttcacgctgc cagccgtcgt cgcgcgaatc ttcatagtct cgcccacggt
    12481 cagggttata agcgtcatta atcgcctgct gaatattacc aatagtatcg tcgataatat
    12541 cagcctgggc cggaacggtg gacagcgtga gcagggtgaa taaaagaaat agcggaaagc
    12601 gtttcattag ccaacctcaa aaagaaactc tatccacatt aatcattact catccatgca
    12661 agtagtggat gaatctcaat ttctccgctg ctctattgcc gtaatcgcct ccacgcgttg
    12721 ttgatgacga ccgccttcgt actgtgcgcc cagccacgca tccacaatca tttttgccag
    12781 ttcgaggcca accactcgtg aaccaaaagc cagtgcaact tcactggcat aatgcggata
    12841 atcagtgcgc tctgacgacc tggtgtcttt atcaatcacc tcactcattg accatgaata
    12901 aaaggctggc acagcaacgc agaatcctgc ggtgccagcg acatgaccgc ccattgctcc
    12961 ggtacgtttt ttaaaaagaa acaccggttg cattgataaa ttgtgctcag ta
//
LOCUS       DAPGWD010000116        13004 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPGWD010000116 DAPGWD010000000
VERSION     DAPGWD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.117"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1146
                     /gene="proV"
                     /locus_tag="RHX88_003246"
     CDS             <1..1146
                     /gene="proV"
                     /locus_tag="RHX88_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDV2231908.1"
                     /translation="RAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLS
                     GSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHM
                     TVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARAL
                     AINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAI
                     MQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFG
                     PRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQ
                     TPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGINNG"
     gene            1139..2203
                     /gene="proW"
                     /locus_tag="RHX88_003247"
     CDS             1139..2203
                     /gene="proW"
                     /locus_tag="RHX88_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDV2231909.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2260..3252
                     /gene="proX"
                     /locus_tag="RHX88_003248"
     CDS             2260..3252
                     /gene="proX"
                     /locus_tag="RHX88_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDV2231910.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3543..4727
                     /locus_tag="RHX88_003249"
     CDS             3543..4727
                     /locus_tag="RHX88_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2231911.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFTNFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4851..5588
                     /gene="ygaZ"
                     /locus_tag="RHX88_003250"
     CDS             4851..5588
                     /gene="ygaZ"
                     /locus_tag="RHX88_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDV2231912.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5578..5913
                     /gene="ygaH"
                     /locus_tag="RHX88_003251"
     CDS             5578..5913
                     /gene="ygaH"
                     /locus_tag="RHX88_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDV2231913.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6004..6534
                     /gene="emrR"
                     /locus_tag="RHX88_003252"
     CDS             6004..6534
                     /gene="emrR"
                     /locus_tag="RHX88_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDV2231914.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6661..7833
                     /gene="emrA"
                     /locus_tag="RHX88_003253"
     CDS             6661..7833
                     /gene="emrA"
                     /locus_tag="RHX88_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDV2231915.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7850..9388
                     /gene="emrB"
                     /locus_tag="RHX88_003254"
     CDS             7850..9388
                     /gene="emrB"
                     /locus_tag="RHX88_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDV2231916.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9452..9967)
                     /gene="luxS"
                     /locus_tag="RHX88_003255"
     CDS             complement(9452..9967)
                     /gene="luxS"
                     /locus_tag="RHX88_003255"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDV2231917.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(10117..11673)
                     /gene="gshA"
                     /locus_tag="RHX88_003256"
     CDS             complement(10117..11673)
                     /gene="gshA"
                     /locus_tag="RHX88_003256"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV2231918.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11746..12174)
                     /locus_tag="RHX88_003257"
     CDS             complement(11746..12174)
                     /locus_tag="RHX88_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2231919.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12171..12737)
                     /gene="yqaB"
                     /locus_tag="RHX88_003258"
     CDS             complement(12171..12737)
                     /gene="yqaB"
                     /locus_tag="RHX88_003258"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDV2231920.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         2987 a         3460 c         3342 g         3215 t
ORIGIN      
        1 cgagcgttca aatatatcga acaaggactt tcgaaagaac aaattctgga aaaaactggg
       61 ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat atttgtcatc
      121 atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg cctgattgaa
      181 cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc cgatgccgaa
      241 ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc cttaatgccg
      301 catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat taatgccgaa
      361 gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac
      421 agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta
      481 gcgattaatc cggatatatt attaatggac gaagccttct cggcgctcga tccattaatt
      541 cgcaccgaga tgcaggatga actggtaaaa ttacaggcga aacatcagcg caccattgtc
      601 tttatttccc acgatctcga tgaggctatg cgtattggcg accgaattgc cattatgcaa
      661 aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc ggcgaatgat
      721 tatgtccgta ccttcttccg tggcgttgat attagtcagg tattcagtgc gaaagatatt
      781 gcccgccgga caccgaatgg cttaattcgt aaaacccctg gcttcggccc acgttcggca
      841 ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg cggtaataag
      901 tttgtcggcg cagtttccat cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt
      961 gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct tagcgagttg
     1021 ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga ccaacagtat
     1081 gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg gataaataat
     1141 ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg tgcaatccgc
     1201 agacgcctgg ggtacaccgg cgactgcacc gactgacggc ggtggtgctg actggctgac
     1261 cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc ataaaacgct
     1321 gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc atttccgtcc
     1381 cgtcttccag ggcgtgcgcg ttccggttga ttacattctc aacggtttcc agcaattgct
     1441 gctgggtatg cccgcaccgg tggcgattat cgttttcgct ctcatcgcct ggcagatttc
     1501 cggggtcgga atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg caatcggtgc
     1561 ctggtcgcag gccatggtta ccctggcgct ggtgttaacc gccctgctgt tctgtatcgt
     1621 cattggtttg ccgctgggga tctggctggc gagaagtccg cgagcggcga aaattattcg
     1681 tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtgc caatcgtcat
     1741 gctatttggt atcggtaacg tgccgggcgt ggtggtgacg atcatctttg ctctgccgcc
     1801 gattatccgt ctgaccattc tggggattaa ccaggttccg gcggatctga ttgaagcctc
     1861 gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac cgctggcgat
     1921 gccgaccatt atggcgggcg ttaaccagac gctgatgctg gccctttcta tggtggtcat
     1981 tgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta tcggtcgtct
     2041 ggatatgggg cttgccaccg ttggcggcgt cgggattgtg atcctcgcca ttatcctcga
     2101 ccgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac
     2161 cactggccct gtcggtctgc tgacccgccc attcattaag taactctgca cttgcccggt
     2221 aacgccgggc atatcaccct gccaaaaaaa ggaataacaa tgcgacatag cgtacttttt
     2281 gcgacagcgt ttgccacgct tatctctaca caaacttttg ctgccgacct gccgggcaaa
     2341 ggcattacag ttaatccagt tcaaagcacc atcaccgaag aaaccttcca gacactgctg
     2401 gtcagtcgtg cgctggagaa attaggttat accgtcaata aacccagcga agtagattac
     2461 aacgttggct acacctcgct tgcttccggc gatgcaacct tcaccgccgt gaactggacg
     2521 ccgctgcatg acaacatgta cgaagctgcc ggtggcgata agaaatttta tcgtgaaggg
     2581 gtatttgtta acggcgcggc acagggttac ctgatcgata agaaaaccgc cgaccagtat
     2641 aaaatcacca acatcgcaca actgaaagat ccgaagatcg ccaaactgtt cgataccaac
     2701 ggcgacggta aagcggattt aaccggttgt aaccctggct ggggctgcga aggtgccatc
     2761 aaccaccagc ttgccgcgta tgaactgacc aacaccgtga cgcataatca ggggaactac
     2821 gcagcgatga tggccgacac catcagtcgc tacaaagagg gcaaaccggt gttttattac
     2881 acctggacgc cgtactgggt gagtaatgag ctgaagccag ggaaagatgt ggtctggttg
     2941 caggtgccgt tctccgcact gccgggcgat aaaaatgccg ataccaaact gccgaatggc
     3001 gcgaattacg gcttcccggt cagcaccatg catatcgttg ccaacaaagc ctggaccgag
     3061 aaaaatccgg cggcagccaa actgtttgcc attatgcagt tgccagtggc cgatattaac
     3121 gcccagaacg ccattatgca tgacggcaaa gcctcagaag gcgatattca gggccacgtt
     3181 gatggctgga tcaaagccca ccagcagcag ttcgatggct gggtgaatga ggcgctggca
     3241 gcgcagaagt aatttttatt caggcggata aggcgttttt gcctcatccg ccgctctgta
     3301 cacaatgcct gatgcgacgc tgacgcgtct tatcatgcct acaaaataca ctcattcgta
     3361 tggcggataa ggcgttttcg ccgcatccgc cgttctgtgc acaatgcctg atgcgacgct
     3421 ggcgcgtctt atcatgccta caaaccacat cgcacatttc aacaatctat ctttcatccc
     3481 atattcatca acatccgcta ttattgattt ccagcttaat catcacctga tgaacaaaaa
     3541 taatgactaa acctaatcat gagcttagcc cggcgctgat cgtgctgatg tctatcgcca
     3601 ccggtctggc ggtagccagt aactattacg cccagccatt gctcgacacc atcgcgcgta
     3661 acttttccct ttccgccagt tcggcaggct ttattgttac cgccgcgcag ttgggctacg
     3721 ccgcaggtct actgtttctt gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg
     3781 tctcgatgac cttactggca gcgggcggta tgttaatcac tgccagcagt cagtcactgg
     3841 cgatgatgat cctcggtacg gcattaaccg gtttattctc agtcgtggca caaattctgg
     3901 ttccgctggc agcgacgctg gcttcaccgg acaaacgcgg caaagtggtt ggcactatta
     3961 tgagcggtct gctgttgggg atcttactcg cacggaccgt tgccggattg ctggcgaatc
     4021 tcggcggctg gcgcaccgtc ttttgggttg cctcggtgtt aatggcactg atggcgctgg
     4081 cattatggcg tggtctgcca caaatgaaat cagaaaccca cctcaactac ccacagttgt
     4141 taggttccgt tttcagcatg tttatcagcg ataagatcct gcgcacccgc gcattgctgg
     4201 gctgcctgac ctttaccaac ttcagcattc tctggacctc aatggccttt ttgcttgccg
     4261 ctccaccttt taactacagc gatggtgtca ttggtctgtt tggacttgcg ggagctgccg
     4321 gagcgttggg cgctcgtccg gcgggcggtt ttgccgataa gggtaaatcg caccacacca
     4381 caactttcgg tctgctgctg ctattacttt catggctggc gatctggttt ggtcacactt
     4441 ccgtactggc gctgattatc ggcatcctgg tgctggatct caccgtacag ggcgtgcata
     4501 tcactaacca gacggtaatt tatcgaatac accctgatgc gcgtaatcgc ctgaccgcag
     4561 gttacatgac cagctacttt attggcggtg ccgccggttc gctaatttca gcctcagcct
     4621 ggcaacatgg cggttgggct ggcgtttgtc tggctggcgc gacgattgcc ctggttaact
     4681 tactggtctg gtggcgaggt tttcatcgtc aggaagccgc aaattaagca atcagcaacc
     4741 gttttcgtgg ctttacactt ataagggtgt taagaagccc atcagtctga taaggttaag
     4801 atattcattc agtctattta taatattaac aatcgttaag cgtacactct atggaaagcc
     4861 ctactccaca gcctgctcct ggttcggcga ccttcatgga aggatgcaaa gacagtttac
     4921 cgattgttat tagttatatt ccggtggcct ttgcgttcgg tctgaatgcg acccgtctgg
     4981 gattctctcc tctcgaaagc gtttttttct cctgcatcat ttatgcaggc gcgagccagt
     5041 tcgtcattac cgcgatgctg gcagccggga gtagtttgtg ggttgctgca ctgaccgtca
     5101 tggcaatgga tgttcgccat gtgttgtatg gcccgtcact gcgtagccgt attattcagc
     5161 gtctgcaaaa atcgaaaacc gccctgtggg catttggcct gacggatgag gtttttgccg
     5221 ccgcaaccgc aaaactggta cgcaataatc gccgctggag cgagaactgg atgatcggca
     5281 ttgccttcag ttcatggtca tcgtgggtat ttggtacggt aataggggca ttctccggca
     5341 gcggcttgct gcaaggttat cccgccgttg aagctgcatt aggttttatg cttccggcac
     5401 tctttatgag tttcctgctc gcctctttcc agcgcaaaca atctctttgc gttaccgcag
     5461 cgttagttgg tgcccttgca ggcgtaacgc tattttctat tcccgtcgcc attctggcag
     5521 gcattgtctg tggctgcctc actgcgttaa tccaggcatt ctggcaagga gcgcccgatg
     5581 agctatgagg ttctgctgct tgggttactg gttggcgtgg cgaattattg cttccgctat
     5641 ttgccgctgc gcctgcgtgt gggtaatgcc cgcccaacca aacgtggcgc ggtaggtatt
     5701 ttgctcgaca ccattggcat cgcctcgata tgcgctctgc tggttgtctc taccgcacca
     5761 gaagtgatgc acgatacacg ccgtttcgtg cccacgctgg tcggcttcgc ggtactgggt
     5821 gccagtttct ataaaacacg cagcattatc atcccaacac tgcttagtgc gctggcctat
     5881 gggctcgcct ggaaagtgat ggcgattata taacccacaa gaatcatttt tctaaaacaa
     5941 tacatttact ttatttgtca ctgtcgttac tatatcggct gaaattaatg aggtcatacc
     6001 caaatggata gttcgtttac gcccattgaa caaatgctaa aatttcgcgc cagccgccac
     6061 gaagattttc cttatcagga gatccttctg actcgtcttt gcatgcacat gcaaagcaag
     6121 ctgctggaga accgcaataa aatgctgaag gctcagggga ttaacgagac gttgtttatg
     6181 gcgttgatta cgctggagtc tcaggaaaac cacagtattc agccttctga attaagttgt
     6241 gctcttggat catcccgtac caacgcgacg cgtattgccg atgaactgga aaaacgcggt
     6301 tggatcgaac gtcgtgaaag cgataacgat cgccgctgcc tgcatctgca attaacggaa
     6361 aaaggtcacg agtttttgcg cgaggtttta ccaccgcagc ataactgcct gcatcaactc
     6421 tggtccgcgc tcagcacaac agaaaaagat cagctcgagc aaatcacccg caaattgctc
     6481 tcccgtctcg accagatgga acaagacggt gtggttctcg aagcgatgag ctaacgcgtc
     6541 atctcgctca aaaatccaga tttataaaag aaaaaatgac cggccagcat cgcaacatgc
     6601 tggccttttt ggcaagcagg tcggctcagc cgatgagtta agaagatcgt ggagaacaat
     6661 atgagcgcaa atgcggagac tcaaaccccg cagcaaccgg taaagaagag cggcaaacgt
     6721 aagcgtctgc tcctccttct caccttgctc tttataatta ttgccgtagc gatagggatt
     6781 tattggtttt tggtactgcg tcatttcgaa gaaaccgatg acgcatacgt ggcagggaat
     6841 cagattcaaa ttatgtctca ggtgtctggc agcgtgacga aagtctgggc cgataacacc
     6901 gattttgtaa aagaaggcga tgttctggtc actctcgacc cgacagatgc tcgccaggcg
     6961 tttgaaaaag ccaaaactgc actggcttcc agcgttcgcc aaacccacca gctgatgatt
     7021 aacagcaaac agttgcaggc gaatattgag gtgcaaaaaa tcgccctcgc gaaagcacaa
     7081 agcgactaca accgccgtgt gccgctgggc aatgccaacc tgattggtcg cgaagagctg
     7141 caacacgccc gcgacgccgt caccagtgcc caggcgcaac tggacgtcgc tattcaacaa
     7201 tacaatgcca atcaggcgat gattctgggg actaaactgg aagatcagcc tgccgtgcaa
     7261 caggctgcca ccgaagtacg taacgcctgg ctggcgctgg agcgtacccg tattgtcagt
     7321 ccgatgaccg gttatgtctc ccgccgcgcg gtacagcctg gggcgcaaat tagcccaacg
     7381 acgccgctga tggcggtcgt tccagccacc aatatgtggg tggatgccaa ctttaaagag
     7441 acgcagattg ccaatatgcg tatcggtcag ccggtcacca tcaccaccga tatttacggc
     7501 gatgatgtga aatacaccgg taaagtggtt ggtctggata tgggcacagg tagcgcgttc
     7561 tcactgcttc cggcgcaaaa tgcgaccggt aactggatca aagtcgttca gcgtctacct
     7621 gtgcgtatcg aactggacca gaaacagctg gagcaatacc cgctgcgcat tggtttgtcc
     7681 acgctggtga gcgtcaatac cactaaccgt gacggtcagg tactggcaaa taaagtacgt
     7741 tcaacaccgg tcgcggtaag taccgcgcgt gaaatcagcc tggcacctgt caataaactg
     7801 atcgacgata tcgtaaaagc taacgctggc taatccagag gtgcgtgtga tgcaacagca
     7861 aaaaccgctg gaaggcgcgc aactggtcat tatgacgatt gcgctgtcac tggcgacatt
     7921 catgcaggtg ctggactcca ccattgctaa cgtggcgatc cccactatcg ccgggaatct
     7981 gggctcatcg ctcagccagg gaacgtgggt aatcacttct ttcggggtgg cgaatgccat
     8041 ctcgatcccg cttaccggct ggctggcaaa gcgcgtcggg gaagtgaaac tgttcctttg
     8101 gtccaccatc gcctttgcta ttgcgtcgtg ggcgtgtggt gtctccagca gcctgaatat
     8161 gctgatcttc ttccgcgtga ttcaggggat tgtcgccggg ccgttgatcc cgctttcgca
     8221 aagtctattg ctgaataact acccgccagc caaacgctcg atcgcgctgg cgttgtggtc
     8281 gatgacggtg attgtcgcgc caatttgcgg cccgatcctc ggcggttata tcagcgataa
     8341 ttaccactgg ggctggatat tcttcatcaa cgtgccgatt ggcgtggcgg tggtgttgat
     8401 gacactgcaa actctgcgcg gacgtgaaac ccgcaccgaa cggcggcgga ttgatgccgt
     8461 ggggctggca ctgctggtta ttggtatcgg cagcctgcag attatgctcg accgcggtaa
     8521 agagctggac tggttctcat cacaggaaat tatcattctt accgtggtgg cagtggtggc
     8581 tatctgcttc ctaattgtat gggagctgac cgacgataac ccgatagtcg atctgtcgtt
     8641 gtttaagtcg cgcaacttca ccatcggctg cttgtgtatc agcctcgcgt atatgctcta
     8701 cttcggcgcg attgttctgc tgccgcagtt gttgcaggag gtctacggtt acacggcaac
     8761 gtgggcaggt ctggcctctg cgccggtagg gattattccg gtgatcctgt cgccgattat
     8821 cggccgcttc gcgcataaac tggatatgcg gcggctggta accttcagct ttattatgta
     8881 tgccgtctgc ttctactggc gtgcctatac ctttgaacca ggtatggatt ttggcgcgtc
     8941 ggcctggccg cagtttatcc aggggtttgc ggtggcctgc ttctttatgc cgctgaccac
     9001 cattacgctg tctggtttgc caccggaacg actggcggcg gcatcgagcc tctccaactt
     9061 tacgcgtacg ctggcggggt ctatcggcac gtccatcacc accaccatgt ggaccaaccg
     9121 cgagtcgatg caccacgcac agttaaccga gtcggtaaac ccgtttaacc caaacgccca
     9181 ggcgatgtac agtcaactgg aagggcttgg gatgacgcaa cagcaggcct ccggctggat
     9241 tgcccagcag atcaccaacc aggggctgat tatttccgcc aatgagatct tctggatgtc
     9301 agccgggata ttcctcgtcc tgctggggct ggtgtggttt gctaaaccgc catttggcgc
     9361 aggtggcggc ggaggcggtg cgcactaagt acaactaagc cagttcattt gaactggctt
     9421 ttttcaatta attgtgaaga tagtttactg actaaatgtg cagttcctgc aacttctctt
     9481 ccggcagcgc cagttcttcg ttgctgttga tgcgcacgtc acgttccaga atgctacgcg
     9541 caatatcctg cgcttcctgc aacgagtgca tctggtaagt gccacactgg tagacgttca
     9601 gttccgggat ctgattctga tcctgcactt tcagcacgtc ttccattgcc gctttccagg
     9661 catcagcaac acgctgctca tctggcgtac caatcagact catataaaaa ccggtgcggc
     9721 agcccattgg cgagatatcg ataatctcta caccattacc gttaagatgg ttacgcataa
     9781 aaccagcaaa caggtgctcc agggtatgga tccctctttc tggcatcact tctttgttcg
     9841 gcacgcagaa gcgcagatcg aacacggtga ttgcgtcgcc atgcggggtg ttcattgttt
     9901 tcgccacccg aactgcaggc gcttccatcc gggtatgatc gactgtgaag ctatctaaca
     9961 acggcattta gccacctccg gtaatttttt taaaaatttt ctgaactctt tcttcccagg
    10021 cgagtctgag tatatgaaag acgcgcattt gttatcatca tccctgaatt cagagatgaa
    10081 attttggcca ctcacgagtg gcctttttct tttctgtcag gcgtgttttt ccagccacac
    10141 cgcaaacggt tcggtatcag cggcttccat ttcctgctga cggcgttcag acgcctcgcg
    10201 ctcggctaca aaatcctctt cgcgcagaat ttccagcggc tcttcacgca gcagattacg
    10261 gtaggcttct gcaaatgctt tgcctgttcc gccaatacca gtatcaatca tagaccttaa
    10321 gatacgggca gagaaagtca gatcgggatt atcgaagcag gcaaccagtt catcacacac
    10381 tttctgatac gcttcgccgc cgttaatact atccagcgtt tgcgcgacgc gtttcagatc
    10441 gcggaacaga tctttaccca cctgcggtaa cgggaactgt gcggtttcgc agccgatacc
    10501 cagcgtcaga cccggtttgc gaccttcgag gatcacccgg ttccagttaa cgcgtgtaca
    10561 ggcaagttcg ctactgctca tttccggtgc atcagccagc gcacaccaga ccataaacag
    10621 gtcgaggaat cgcacctgct gttcatctac accaatcggc gagaacgggt tgatgtccag
    10681 cgaacgcact tcaatatatt caatgccgcc acgtaacagc gcatcagaag gcgactcgcc
    10741 gctgcgggta acgcgttttg gacgaatcgg cgcgtacagt tcgttttcaa tctgcaacac
    10801 gttgctgttg atttgcagcc tcttaccgtc tttctcaata ccaatcttcg cgtactcttc
    10861 cgatggcgtt ttgattgcct gtttaaggcc cgctacgtac tcgtaaagat cgttgaaggt
    10921 aataccaaga ttgctttgcg atttattggt atagccgaga tcgctcaaac gaagagaggt
    10981 cgcatacggc aggtaataca taccgcactc ggttttctca aacggcagcg atgttggttt
    11041 tccttgcagg aaagaagaac aaatcgccgg agatgcacca aacagataag gaatgaccca
    11101 accgaaacga tagtaattgc ggataacgcg gaaatagccc gcagaaattt tctctttggc
    11161 atcagcgccc gagatatcac cgcacttcgc ttgccagaat gccattggta aagagaaatt
    11221 gtagtgcacg ccggaaatgg tttgcatcag cgcgccgtag cgatttttca gcccttcacg
    11281 gtacagcgtt ttaaagcgtc cggtgttaga agtgccgtac tgtgccagtt cgatgtcctg
    11341 accttctgcg atgtagcatg gcatacttaa cggccacatc cgctcatcgc ccatattgcg
    11401 cgccgtataa cgatgcagat cgcgcataaa ggtcagcata tgttcaatat caccatccac
    11461 tggtgtaatg aattccagca atgcttccgc aaaatcggta gtaatccatt tatgcgtcag
    11521 tgcggaacct aatgcttcag gatgacctgt tgttgccagt gtgccatcag cattaacacg
    11581 caaagtttcg cgctccagcc cacgctgtat cccctttaac gcctgaggat gtttttccag
    11641 ccaggccagc gcctgtgata cgtccgggat caaattgacc tcccgcctgt caaaatcgtt
    11701 ttaattagca taactgtaat ggtgaccata tgtgcaggcc tacaattagt gccaccacat
    11761 catgccctga acggtcgctg ctgcaactgc aacatagcgt aacgctttac caaggcacaa
    11821 aaaaaagatt actggccccc aggagatgcg catccatccc gctaacagac acagtaaatc
    11881 gccaaccacg ggcatccagc ttaataatag cgtgactgca ccatagcgtt tcagccagcc
    11941 ggtagctttc tcttgccagc gcgatgtttt acgcaatgga aagaaacgcc caaggataac
    12001 gttagttaac cctccaaggc tattacccat tgttgctgtt aaaactaaaa cccagggata
    12061 actgatcccg gaaagcaaca ttgccaccag cacgacttcg gagttgccgg gtaatagtgt
    12121 agcgctgaga aaactactgg caaacaacga gaaaagcgat aacgcttcac tcacagcaag
    12181 cgaacatcca cggcgtccat gcctgctgca cgggccgcct gaataccgaa atcggcatct
    12241 tcaaagacca cacactgcgt cggttgcacg cccatacgct gcgcgcacaa caaaaatgtg
    12301 tctggcgcgg gtttatggtg tttgacgtga tcagcagcga cgacggcgtc aaaataacgg
    12361 cgtaatccca ggtgcgccag caatgcctca gcgatggcgc tttcactccc cgttcctaca
    12421 gccattgggc gacgaccatg ccaacttttc accacttcaa caagaggaag cggctcgaca
    12481 ctatccagca gcatacttct tactgcttct gttttttcac gcgctaacgc atgcgggtcg
    12541 agatcggcct gattcagctc aataattgcc tgagcaatac gccaggtggg cgatccatta
    12601 agcgcaatca ttgcctgaac atcgtactga agaccgtagt gccctaatac ttcgcgccac
    12661 gctttacggt gcgtaggctc cgtatccagg attgtgccat ccatatcaaa aattaaacct
    12721 gcataacgct cgtacatggt cttctcgcaa gtcgaaaaat atgacgctac tttagcgtaa
    12781 ttgcttgatt ttgtcgctga tggcgaagga gaagaaaatt caggaaatgg ataaagtagt
    12841 tatttagaaa tgagatattt ttgaaggaaa tttttgggag aagatggtgc atccgggagg
    12901 attactcact ggcgttcgcc cttcgggccg ttgctgacgc aacgttatcc tccctggtgc
    12961 ctgcggttaa taccgctatc tcttcgatac cttcattgct gaag
//
LOCUS       DAPGWD010000117        12874 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPGWD010000117 DAPGWD010000000
VERSION     DAPGWD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.118"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            161..1270
                     /gene="livK"
                     /locus_tag="RHX88_003259"
     CDS             161..1270
                     /gene="livK"
                     /locus_tag="RHX88_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDV2231921.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1318..2244
                     /gene="livH"
                     /locus_tag="RHX88_003260"
     CDS             1318..2244
                     /gene="livH"
                     /locus_tag="RHX88_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDV2231922.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2241..3518
                     /gene="livM"
                     /locus_tag="RHX88_003261"
     CDS             2241..3518
                     /gene="livM"
                     /locus_tag="RHX88_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDV2231923.1"
                     /translation="MKPMHIAMALLSAAMFFILAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQILRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3515..4282
                     /gene="livG"
                     /locus_tag="RHX88_003262"
     CDS             3515..4282
                     /gene="livG"
                     /locus_tag="RHX88_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDV2231924.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4284..4997
                     /gene="livF"
                     /locus_tag="RHX88_003263"
     CDS             4284..4997
                     /gene="livF"
                     /locus_tag="RHX88_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDV2231925.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5393..6709
                     /gene="ugpB"
                     /locus_tag="RHX88_003264"
     CDS             5393..6709
                     /gene="ugpB"
                     /locus_tag="RHX88_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDV2231926.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6807..7694
                     /gene="ugpA"
                     /locus_tag="RHX88_003265"
     CDS             6807..7694
                     /gene="ugpA"
                     /locus_tag="RHX88_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDV2231927.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7691..8536
                     /gene="ugpE"
                     /locus_tag="RHX88_003266"
     CDS             7691..8536
                     /gene="ugpE"
                     /locus_tag="RHX88_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDV2231928.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTYLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8538..9608
                     /gene="ugpC"
                     /locus_tag="RHX88_003267"
     CDS             8538..9608
                     /gene="ugpC"
                     /locus_tag="RHX88_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDV2231929.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9605..10348
                     /gene="ugpQ"
                     /locus_tag="RHX88_003268"
     CDS             9605..10348
                     /gene="ugpQ"
                     /locus_tag="RHX88_003268"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV2231930.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGESLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10335..10775)
                     /locus_tag="RHX88_003269"
     CDS             complement(10335..10775)
                     /locus_tag="RHX88_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDV2231931.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10895..12637
                     /gene="ggt"
                     /locus_tag="RHX88_003270"
     CDS             10895..12637
                     /gene="ggt"
                     /locus_tag="RHX88_003270"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDV2231932.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPKHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12675..>12874)
                     /locus_tag="RHX88_003271"
     CDS             complement(12675..>12874)
                     /locus_tag="RHX88_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231933.1"
                     /translation="FSQGWYFCFEAREFLETGDEAARLAGNAPFIIDKDSGEIHSLGT
                     AKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2833 a         3311 c         3715 g         3015 t
ORIGIN      
        1 atcgactgaa aaatagggat tttaatcact attatcacaa aatactgcgc taaaccctta
       61 atcagacagg caaaaacagt gcagtataaa aaaagaacag tctgatttgt taacacataa
      121 aaacaaagca acacaacatc acgaatgggg atttttgact atgaaacgga atgcgaaaac
      181 tatcatcgca gggatgattg cactggcaat ttcacacacc gctatggctg acgatattaa
      241 agttgccgtt gtcggcgcga tgtccggccc gattgcccag tggggcgata tggaatttaa
      301 cggcgcgcgt caggcgatta aagacattaa tgccaaaggg ggaattaaag gcgataagct
      361 ggtgggcgtg gaatatgacg acgcctgcga cccgaaacaa gccgttgcgg tcgccaacaa
      421 aatcgttaac gacggcatta aatacgttat tggtcatctg tgttcttctt ctacccaacc
      481 tgcgtcagat atctacgaag acgaaggtat tctgatgatc tcgccgggag cgaccaaccc
      541 ggagctgacc caacgcggtt atcaacacat tatgcgtact gccgggctgg actcttccca
      601 ggggcctacg gcggcaaaat acattcttga gacggtgaag ccccagcgca tcgccatcat
      661 tcacgacaaa caacagtatg gcgaagggct ggcgcgttcg gtgcaggacg ggctaaaagc
      721 ggctaacgcc aacgtcgtct tcttcgacgg tattaccgcc ggggagaaag atttctccgc
      781 gctgatcgcc cgcctgaaaa aagaaaacat cgacttcgtt tactacggcg gttactaccc
      841 ggaaatgggg cagatgctgc gccaggcccg ttccgttggc ctgaaaactc agtttatggg
      901 gccggaaggt gtgggtaacg catcattgtc gaatattgcc ggtgatgctg ccgaaggcat
      961 gttggtcact atgccaaaac gctatgacca ggatccggca aaccagggca tcgttgatgc
     1021 gctgaaagca gacaagaaag atccgtccgg gccttatgtc tggatcacct acgcggcggt
     1081 gcaatctctg gcgactgccc ttgagcgtac tggcagcgat gagccgctgg cgctggtgaa
     1141 agatttaaaa gctaacggtg caaacaccgt gattgggccg ctgaactggg atgaaaaagg
     1201 cgatcttaag ggatttgatt ttggtgtctt ccagtggcac gccgacggtt catccacggc
     1261 agccaagtga tcatcccacc gcccgtaaaa tgcgggcggg tttagaaagg ttaccttatg
     1321 tctgagcagt ttttgtattt cttgcagcag atgtttaacg gcgtcacgct gggcagtacc
     1381 tacgcgctga tagccatcgg ctacaccatg gtttacggca ttatcggcat gatcaacttc
     1441 gcccacggcg aggtttatat gattggcagc tacgtctcat ttatgatcat cgccgcgctg
     1501 atgatgatgg gcattgatac cggctggctg ctggtggcgg cgggattcgt cggcgcaatc
     1561 gtcattgcca gcgcctacgg ctggagtatc gaacgggtag cctatcgccc ggtgcgtaac
     1621 tctaagcgcc tgattgcgct catctcggca atcggcatgt ccatcttcct gcaaaactac
     1681 gttagcctga ccgaaggttc gcgtgacgtg gcgctgccca gtctgtttaa cgggcagtgg
     1741 gtggtgggcc atagcgaaaa cttctctgcc tctattacta ccatgcaggc ggtgatttgg
     1801 attgttacct tcctcgccat gctggcgctg acgattttca ttcgctattc ccgtatgggg
     1861 cgcgcctgtc gtgcctgcgc ggaagatctg aaaatggcga gcctgcttgg catcaacacc
     1921 gaccgggtga ttgcgctgac ctttgtgatt ggcgcggcga tggcggcggt ggcgggtgtg
     1981 ctgctcggtc agttctacgg tgtgattaac ccctacatcg gctttatggc cgggatgaaa
     2041 gcctttaccg cggcggtgct cggcgggatt ggcagcattc cgggggcgat gattggcggg
     2101 ctgatcctgg ggattgcgga ggcgctctct tctgcctatc tgagtacgga atataaagat
     2161 gtggtctcat ttgccctgct gatcctggta ctgctggtga tgccgaccgg tattctgggg
     2221 cgcccggagg tagagaaagt atgaaaccga tgcatattgc aatggcgctg ctctctgccg
     2281 cgatgttctt tatcctcgct ggcgtcttta tgggcgtaca gctggagctg gacggcacca
     2341 aactggtggt cgacacggct tcggatgtcc gttggcagtg ggtgtttatc ggcacggcgg
     2401 tggtcttttt cttccagatt ttgcgacctg ctttccagaa agggttgaaa agcgtttccg
     2461 ggccgaagtt tattctgccc gcgattgatg gctccacggt gaagcagaaa ctgttcctcg
     2521 tggcgctgtt ggtgcttgcg gtggcgtggc cgtttatggt ttcacgcggg acggtggata
     2581 tcgccaccct gaccatgatc tacattatcc tcggcctcgg gctgaacgtg gtggtagggc
     2641 tttccggcct gctggtgctg gggtacggcg gtttttacgc catcggcgct tacacttttg
     2701 cgctgctcaa tcactattac ggcttgggct tctggacctg tctgccgatt gccgggttaa
     2761 tggcagcggc ggcgggcttc ctgctcggct ttccggtgct gcgtctgcgt ggtgattatc
     2821 tggcgatcgt taccctcggc ttcggcgaaa ttgtgcgcat attgctgctc aataacaccg
     2881 aaattaccgg cggcccgaac ggaatcagtc agatcccgaa accgactttc ttcggccttg
     2941 agttcagccg taccgcccgt gaaggcggtt gggacacgtt cagtaatttc tttggcctga
     3001 aatacgatcc ctccgatcgc gtcatcttcc tgtatctggt ggcgttgctg ctggtggtgc
     3061 taagcctgtt tgtcattaac cgcctgctgc ggatgccgct ggggcgtgcg tgggaagcgt
     3121 tgcgtgaaga tgagatcgcc tgccgttcgc tgggcttaag cccgcgtcgt atcaagctga
     3181 ccgcttttac catcagtgcc gcgtttgccg gttttgccgg aacgctgttt gctgcgcgcc
     3241 agggctttgt cagcccggaa tccttcacct ttgctgaatc ggcgtttgta ctggcgatag
     3301 tggtgctcgg cggtatggga tcgcagtttg cggtgattct ggcggcaatt ttgctggtgg
     3361 tgtcgcgcga gttgatgcgt gatttcaacg aatacagcat gttaatgctc ggtggtttga
     3421 tggtgctgat gatgatctgg cgtccgcagg gcttgctgcc catgacgcgc ccgcaactga
     3481 agctgaaaaa cggcgcagcg aaaggagagc aggcatgagt cagccattat tatctgtgaa
     3541 cggcctgatg atgcgcttcg gcggcctgct ggcggtgaac aacgtcaatc ttgaactgta
     3601 cccgcaggag atcgtctcgt taatcggccc taacggtgcc ggaaaaacca cggtttttaa
     3661 ctgcctgacc ggtttctaca aacccaccgg cggcaccatt ttactgcgcg atcagcatct
     3721 tgaaggttta ccggggcagc aaattgcccg catgggcgtg gtgcgcacct tccagcatgt
     3781 gcgtctgttc cgtgaaatga cggtaattga aaacctgctg gtggcgcagc atcagcaact
     3841 gaaaaccggg ctgttctctg gcctgttgaa aacgccttct ttccgtcgcg cccagagcga
     3901 agcgctcgac cgcgccgcca cctggcttga gcgcattggt ttgctggaac acgccaaccg
     3961 tcaggcgagt aacctggcct atggtgacca acgccgtctg gagattgccc gctgcatggt
     4021 gacgcagccg gagattttaa tgctcgacga accggcggca ggtcttaacc caaaagaaac
     4081 caaagagctg gatgagctga ttgccgaact gcgcaatcat cacaacacca ctatcttgtt
     4141 gattgaacac gatatgaagc tggtgatggg gatttcagac cgaatttatg tggtcaatca
     4201 ggggacgcca ctggcaaacg gtacgccgga gcaaatccgt aataacccgg acgtgatccg
     4261 tgcctattta ggtgaggcat aagatggaaa aagtcatgtt gtcctttgac aaagtcagcg
     4321 cccactacgg caaaatccag gcgctgcatg aggtgagcct gcatatcaat cagggcgaga
     4381 ttgtcaccct cattggcgcg aacggggcgg ggaaaaccac cttgctcggc acgttatgcg
     4441 gcgatccgcg cgctaccagc gggcgaattg tgtttgatga taaagacatt accgactggc
     4501 agacggcgaa aatcatgcgc gaagcggtag cgattgtccc ggaagggcgt cgcgtcttct
     4561 cgcggatgac ggtggaagag aacctggcga tgggcggctt ttttgccgaa cgcgaccagt
     4621 tccaggagcg cataaagtgg gtgtatgaac tgtttccacg tctgcatgag cgccgtgttc
     4681 agcgggcagg caccatgtcc ggcggtgagc aacaaatgct ggcgattggt cgcgcgctga
     4741 tgagcaaccc gcgtttgctg ctacttgatg agccatcgct cggtcttgcg ccgattatca
     4801 tccagcaaat tttcgacacc atcgagcagc tgcgcgagca ggggatgact atcttcctcg
     4861 tcgagcagaa cgccaaccag gcgctaaagc tggcggatcg cggctacgtg ctggaaaacg
     4921 gccatgtggt gctctccgat accggcgacg cgctgctggc gaacgaagca gtgagaagtg
     4981 cgtatttagg cgggtaataa cacgttgatt gataaggagt caaaagactc ctttgagaca
     5041 ggtgacaaat gtaaaattgc ctgatgcgct gcgcttatca ggcctactgg gtgagttgca
     5101 atatattgaa tttgcacgat cttgtaggcc ggataaggcg tttacgccgc atccggcatg
     5161 aaacgatgag taatctgtag agtttgattc agaccttcta ttttccgctt atccgtgccc
     5221 catctcccat tttccctcac ccacgccgtc accgccttgt catctttctg acaccttact
     5281 atcttacaaa tgtaacaaaa aagttatttt tctgtaattc gagcatgtca tgttaccccg
     5341 cgagcataaa acgcgtgaat tcgcgcattc ggtacaacaa gagagataaa cgatgaaacc
     5401 gttacattat acagcttcag cactggcgct cggactggcg ttaatgggga atgcacaggc
     5461 agtgacgacc attccgttct ggcattctat ggaaggggaa ctgggtaaag aggtggattc
     5521 tctggcccaa cgttttaacg ccgaaaatcc ggattacaaa attgtaccga cctataaagg
     5581 caactacgaa cagaatttaa gcgcggggat tgccgcattt cgtaccggca acgctccggc
     5641 tattttgcag gtttatgaag ttggcaccgc caccatgatg gcatcgaaag ccattaaacc
     5701 ggtatatgac gtgtttaaag aggcggggat tcaattcgat gagtcgcagt ttgtgccgac
     5761 ggtttccggt tactactccg acagcaaaac gggccactta ctctcccagc cattcaacag
     5821 ctcgaccccc gttctctatt acaacaaaga cgccttcaag aaagcaggat tagacccgga
     5881 acaaccgccg aaaacctggc aggatctggc ggactatgcc gcgaaactga aagcctccgg
     5941 tatgaagtgc ggctacgcca gcggctggca gggctggatc caactggaaa actttagcgc
     6001 ctggaacggt ctgccgtttg ccagcaaaaa caacggcttt gacggcacgg acgcggtgct
     6061 ggagttcaac aagccggagc aggtgaaaca catcgccatg ctcgaggaga tgaacaagaa
     6121 gggcgacttc agctacgtcg gtcgtaagga tgaatccacc gagaagttct ataacggtga
     6181 ttgcgcgatg accaccgcct cttccggttc tctcgctaat attcgtgagt acgccaaatt
     6241 taactatggc gtaggcatga tgccttacga cgccgatgcg aaagatgcac cgcaaaacgc
     6301 cattatcggc ggggccagcc tgtgggtgat gcagggcaaa aataaagaaa cgtataccgg
     6361 cgtggcgaag ttcctcgact tcctcgcgaa gccagaaaac gctgccgagt ggcatcagaa
     6421 aaccggttat ctgccaatca ccaaagcagc gtatgacctg acccgtgagc agggctttta
     6481 cgagaaaaac ccaggggcgg ataccgcgac gcgtcagatg ctgaataagc cgccgttgcc
     6541 gttcaccaaa gggctgcgtc tgggcaacat gccgcagatc cgcgtgattg tggatgaaga
     6601 gctggagagc gtgtggaccg gtaagaagac accacagcag gcactggata ccgccgttga
     6661 gcgtggaaat cagttgctgc gccgctttga gaaatcgacg aagtcttaat cagtgtaatg
     6721 tcggatgcgt ttcgcttatc tgacctggca tcgcgtgtag gccggataag cgaagcgcat
     6781 ccggcacagt tcaggaatta accgtaatgt catcatcccg tccggtgttc cgctcgcgct
     6841 ggctgcctta tctgctggtc gcgccgcagc tcatcatcac cgttatcttt tttatctggc
     6901 ctgcgggcga agcgttgtgg tactcgctac aaagcgtcga tccgtttggt ttctccagcc
     6961 agtttgtcgg cctggataac ttcgtcgcgc tgtttcatga cagctactat ctcgactcct
     7021 tctggacgac gataaaattc agcacctttg tcaccgtcag cggtttgctg gtgtcgctgt
     7081 tcttcgcggc gttggtggag tacatcgtgc gcggcagccg tttctatcaa accttgatgc
     7141 tgctgccgta tgccgtggct cccgccgttg ccgccgtatt gtggatcttc ctgtttaacc
     7201 ccggtcgtgg gctgatcacc cattttctcg ctgagttcgg ttatgactgg aaccatgcgc
     7261 agaacagcgg tcaggcaatg tttctggtgg tgtttgcctc ggtgtggaag caaatcagct
     7321 acaactttct gttcttctat gccgcgctgc actccattcc ccgttcattg attgaagctg
     7381 cagccatcga cggtgcaggc ccaattcgcc gcttctttaa gattgcgctg ccgcttatcg
     7441 ctccggtgag tttcttcctg ctggtagtga acctggtgta tgccttcttc gacaccttcc
     7501 cggtgatcga cgccgccacg tccggcgggc cggttcaggc caccacgacg ctgatttata
     7561 agatctaccg cgaaggtttt accggactgg atctggcttc gtctgccgca cagtcggtgg
     7621 tgttgatgtt cctcgtcatc gtgctgacgg tggtgcagtt ccgctatgtt gaaagcaagg
     7681 tgcgttacca atgattgaga accgtccgtg gctgacgata ttcagccata ccatgctgat
     7741 cctcgggatc gcggtgatcc tcttcccgct gtacgtggcg tttgtcgcgg cgacgctgga
     7801 taaacaggcc gtctatgccg cgccgatgac gctcatcccc ggcacatatc tgctggaaaa
     7861 tatccacaac atctgggtta acggggtagg tacgaatagc gcgccgttct ggcggatgtt
     7921 gcttaacagc tttgtgatgg cgttcagcat tacgctcggc aaaattaccg tctcgatgct
     7981 ctcggcattt gccattgtct ggtttcgttt tccgctacgt aacctcttct tctggatgat
     8041 ttttatcacc ctgatgctgc cggttgaagt acgtatcttc ccgacggtgg aagtcatcgc
     8101 caacctgaag atgctcgaca gctacgccgg tttaacgctg ccgctgatgg cctcggcgac
     8161 cgctactttc ctgttccgcc agttctttat gacgctgccg gatgagctgg tggaagccgc
     8221 gcggatcgac ggcgcatcgc caatgcgctt cttttgcgac atcgtttttc cgctctccaa
     8281 aactaatctg gcggcgctgt ttgtgatcac ctttatctac ggctggaatc agtatttgtg
     8341 gccgttgttg attattaccg acgttgatct cggcaccacc gtggcaggga tcaaagggat
     8401 gatcgccact ggcgaaggca ccacggaatg gaactcagtg atggcggcga tgttgttaac
     8461 cctgatccca ccagtagtga ttgttttagt gatgcagcgt gccttcgtgc gcggcctggt
     8521 cgatagtgag aaataagatg gcaggactga aattacaggc agtaaccaaa agctgggatg
     8581 gcaaaaccca ggtcattaaa ccgctgaccc ttgatgtggc agatggcgaa tttatcgtga
     8641 tggtcgggcc gtctggctgc gggaaatcga cgctgctgcg catggttgcc gggctggagc
     8701 gggtgaccga aggcgatatc tggatcaacg accagcgcgt gaccgaaatg gagccgaaag
     8761 atcgcgggat tgcgatggtg ttccagaact acgcgcttta tccgcatatg agcgtcgaag
     8821 aaaacatggc gtgggggctg aaaattcgcg gcatgggcaa gcagcaaata gccgagcgcg
     8881 ttaaagaagc ggcgcgcatt ctggagctgg atggcctgct taagcgccgc ccgcgcgagc
     8941 tatccggcgg tcagcgtcag cgtgtggcga tgggtcgcgc gattgtgcgc gatccggcgg
     9001 tgttcctgtt tgatgagccg ctctctaacc tcgatgccaa gctgcgcgtg cagatgcgcc
     9061 ttgagctgca acagctgcac cgtcgcctga aaacgacttc actctacgtt actcacgatc
     9121 aggttgaagc gatgacgctc gcccagcgag taatggtgat gaacggcggc gttgccgaac
     9181 agattggcac accagttgaa gtctacgaaa agcccgccag cctgtttgta gcgagtttta
     9241 tcggcagtcc ggcgatgaac ctgctgacag gccgcgtgaa taacgaaggc acgcatttcg
     9301 aactggacgg cggtattgag ctgccgctaa acggtggcta ccgtcagtat gccgggcgta
     9361 aaatgactct cggcattcgc ccggaacata ttgcgctaag ctcgcaggca gaaggcggcg
     9421 taccgctggt gatggacacg ctggagatcc tcggcgcaga taacctggcg cacggacgct
     9481 ggggcgagca gaagctggtg gtgcgactgg cgcatcagga gcgcccgacg gcaggcagca
     9541 cgctgtggct gcatctggcg gaaaatcagc tgcatctttt tgatggtgaa acaggacaac
     9601 gagtatgagt aactggcctt atccccgcat cgtcgctcat cgtggcggcg gtaagctggc
     9661 cccggaaaac accctggcgg caatcgacgt cggggcaaaa tacggtcata agatgatcga
     9721 atttgacgcg aagttatcga aagatggcga gatcttcctg ctccatgacg acaatctcga
     9781 acgcaccagc aacggctggg gcgtcgcggg tgaactgaac tggcaggatt tactgcgcgt
     9841 ggatgcgggc agttggtaca gcaaagcgtt taaaggtgag tcgctgccgt tgctttcgca
     9901 ggtggcggaa cgctgtcgcg aacacgggat gatggcgaat atcgaaatca aacccaccac
     9961 tggcactgga ccattaacgg gcaaaatggt ggcgctggcg gcacgccaac tgtgggccgg
    10021 tatgacgccg ccgctgctgt catcgtttga gattgatgct ttagaagctg cacagctggc
    10081 ggcaccggaa ctgccgcgtg gtttattgct tgatgagtgg cgcgacgact ggcgcgaact
    10141 gaccgcgcgg ctgggctgcg tctctattca tctcaatcat aagttactcg ataaagtgcg
    10201 agtgatgcag ttgaaagacg ccggactgcg gattctggtt tataccgtca ataaacccca
    10261 gcgcgcggca gagttgctgc gctggggcgt ggattgcatc tgtaccgatg cgattgacgt
    10321 gattggtccg aactttacgg cccaatagtt ttcaacggaa tgtcaggctg cggcgtgctg
    10381 ggctgattca gcatgtcgcc gtttctcctc tccggcagca tatgctgctg attcaacgaa
    10441 ctatccggat tacggttgct gtttaacatc ccgccgttgg tgttgggcag catttgctgc
    10501 cgggcgggat ttcgctcccc cggctgtgac tgcaacaccc gctgagaatt attgtttatc
    10561 tggttttcta aatgctgctg ttgcaactga gtttgcgttt tcagttgctg attcagcatc
    10621 cctttttgct ggatttgctg agtctgcatc tgggtttgca tccgctgctg gctgggtatc
    10681 tgataccccg gctggttagg gttgttcaga gtattaatgg gctgtgcaaa gccgacaaac
    10741 ggcaggagtg ccgtaagaat cagaagtcgt ttcatcgcgt atcctcctct gaagatatcc
    10801 tttaagttta ctcgcttccc gacaaaacga tgattaattc agagttatat accaggctta
    10861 gctggggttg ccccttaatc tctggagaat aacgatgata aaaccgacgt ttttacgccg
    10921 ggtggccatt gctgctctgc tctcaggaag ttgttttagc gccgccgccg cgcctcctgc
    10981 gccgcccgtc tcgtatggtg tggaggaaga tgtcttccac ccggtacgcg cgaaacaggg
    11041 aatggtagcg tctgtggacg ccactgccac tcaggtgggg gtggatattc tcaaggaggg
    11101 cgggaatgcc gttgatgccg ccgtggcggt gggctacgcg ctggcggtaa cgcatccgca
    11161 ggcagggaat ctgggcggtg gtggttttat gctgatccgc tcgaaaaatg gcaataccac
    11221 cgctatcgat ttccgcgaaa tggcacccgc taaagcgacc cgcgatatgt tccttgatga
    11281 ccagggcaac ccggacagca aaaaatcact cacttcgcat ctggcttccg gcacaccagg
    11341 tacggtcgct ggtttctcgc tggcgttgga taagtacggc actatgccgt tgaacaaagt
    11401 ggtgcagccc gcgttcaaac tggcacgcga tggttttatc gttaacgatg ctttggcaga
    11461 cgatctcaaa acctacggta gcgaagtgtt gccgaagcac gaaaacagca aagctatctt
    11521 ctggaaagag ggcgaaccgc tgaaaaaggg cgacacgctg gtgcaggcga acctggcaaa
    11581 gagcctggag atgattgctg aaaacggccc ggacgaattc tataaaggca cgattgcgga
    11641 acagatcgcc caggagatgc agaaaaacgg tggcttgatc actaaagaag atttagcggc
    11701 ctataaagcg gtcgaacgca ctccgataag cggcgattat cgcgggtatc aggtttactc
    11761 catgccaccg ccatcctccg gcgggatcca tatcgtacaa atcctcaata ttctggaaaa
    11821 cttcgatatg aagaaatacg gctttggcag cgccgatgcg atgcaaatca tggcagaagc
    11881 ggagaaatac gcctacgccg accgctcgga atatcttggc gacccggatt ttgtcaaagt
    11941 cccgtggcag gcactgacca ataaagccta tgccaaatcc attgccgatc aaattgatat
    12001 caacaaagcg aagccgtcca gtgagattcg ccctggcaag cttgcgcctt atgagagtaa
    12061 tcaaactacc cattactctg tagtggataa agacggtaac gcggtggcgg tgacctatac
    12121 gctgaacacc accttcggta cgggtattgt cgcgggcgag agcggtattc tgcttaataa
    12181 ccagatggat gatttctccg ccaaaccggg cgtaccgaac gtttacgggc tggtgggcgg
    12241 tgatgccaac gccgtcgggc cgaacaaacg cccgctgtcg tcgatgtcgc cgaccattgt
    12301 ggtgaaagac ggtaaaacct ggctggttac cggtagccca ggcggtagcc ggatcatcac
    12361 tacagtgctg caaatggtgg tgaatagcat cgattatggc atgaacgtcg ccgaagcgac
    12421 caatgcgccg cgtttccacc atcagtggtt gccggacgag ctgcgtgtcg aaaaagggtt
    12481 tagcccggat acgctcaagc tgctggaagc aaaaggtcag aaagtggcgc tgaaagaggc
    12541 gatgggcagt acacaaagca ttatggttgg gccggacggt gagttgtacg gcgcatccga
    12601 cccgcgctcg gtggatgatt taacggcggg gtactaaggt tagcggccct cttcgtggga
    12661 agagggctat tttgtcaggg caagccgaag gtagcctttt ttatttcgta atcctgtaga
    12721 tattcttcca gcggctttgc cgttcccaga gaatgaattt caccactgtc tttatcaata
    12781 ataaaaggtg cgttaccagc taagcgcgcg gcctcatctc cagtttcgag aaattctcgt
    12841 gcttcgaaac agaaatacca gccctggcta aagc
//
LOCUS       DAPGWD010000118        12813 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPGWD010000118 DAPGWD010000000
VERSION     DAPGWD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.119"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            58..1113
                     /gene="phoE"
                     /locus_tag="RHX88_003272"
     CDS             58..1113
                     /gene="phoE"
                     /locus_tag="RHX88_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV2231934.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1152..1553)
                     /gene="crl"
                     /locus_tag="RHX88_003273"
     CDS             complement(1152..1553)
                     /gene="crl"
                     /locus_tag="RHX88_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV2231935.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1611..2855)
                     /gene="frsA"
                     /locus_tag="RHX88_003274"
     CDS             complement(1611..2855)
                     /gene="frsA"
                     /locus_tag="RHX88_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV2231936.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2947..3405)
                     /gene="gpt"
                     /locus_tag="RHX88_003275"
     CDS             complement(2947..3405)
                     /gene="gpt"
                     /locus_tag="RHX88_003275"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV2231937.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3666..5123
                     /gene="pepD"
                     /locus_tag="RHX88_003276"
     CDS             3666..5123
                     /gene="pepD"
                     /locus_tag="RHX88_003276"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV2231938.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5180..5794)
                     /locus_tag="RHX88_003277"
     CDS             complement(5180..5794)
                     /locus_tag="RHX88_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDV2231939.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5791..6930)
                     /locus_tag="RHX88_003278"
     CDS             complement(5791..6930)
                     /locus_tag="RHX88_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDV2231940.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTVTQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7176..7628)
                     /locus_tag="RHX88_003279"
     CDS             complement(7176..7628)
                     /locus_tag="RHX88_003279"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2231941.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7625..8680)
                     /gene="dinB"
                     /locus_tag="RHX88_003280"
     CDS             complement(7625..8680)
                     /gene="dinB"
                     /locus_tag="RHX88_003280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV2231942.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8751..9536)
                     /gene="lafU"
                     /locus_tag="RHX88_003281"
     CDS             complement(8751..9536)
                     /gene="lafU"
                     /locus_tag="RHX88_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV2231943.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTVLLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKRLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            9508..11220
                     /locus_tag="RHX88_003282"
                     /pseudo
     CDS             9508..11220
                     /locus_tag="RHX88_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            11325..11603
                     /locus_tag="RHX88_003283"
     CDS             11325..11603
                     /locus_tag="RHX88_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDV2231944.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            11596..11952
                     /locus_tag="RHX88_003284"
     CDS             11596..11952
                     /locus_tag="RHX88_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDV2231945.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(12009..12782)
                     /locus_tag="RHX88_003285"
     CDS             complement(12009..12782)
                     /locus_tag="RHX88_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDV2231946.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         3171 a         3304 c         3239 g         3099 t
ORIGIN      
        1 ttttcaacgg ggctctcccg ctgaatattc gcgcgttaat taaaatcagg aatgaaaatg
       61 aaaaagagca ctctggcatt agtggtgatg ggcattgtgg catctgcatc tgtacaggct
      121 gcagaaatat ataataaaga cggtaataaa ctggatgtct atggcaaagt taaagccatg
      181 cattatatga gtgataacgc cagtaaagat ggcgaccaga gttatatccg ttttggtttt
      241 aaaggtgaaa cacaaattaa cgatcaactg actggttatg gtcggtggga agcggagttt
      301 gccggaaata aagcggagag tgataccgca cagcaaaaaa cgcgtctcgc ttttgccggg
      361 ttgaaatata aagatttggg ttctttcgat tatggtcgta acctgggcgc gttgtatgac
      421 gtggaagcct ggaccgatat gttcccggaa tttggtggcg attcctcggc gcagaccgac
      481 aactttatga ccaaacgcgc cagcggtctg gcgacgtatc ggaacaccga cttcttcggc
      541 gttatcgatg gcctgaactt aaccctgcaa tatcaaggga aaaacgaaaa ccgcgacgtt
      601 aaaaagcaaa acggcgatgg cttcggcacg tcattgacat atgactttgg cggcagcgat
      661 ttcgccatta gtggggccta taccaactca gatcgcacca acgagcagaa cctgcaaagc
      721 cgtggcacag gcaagcgtgc agaagcatgg gctacaggtc tgaaatacga tgccaataat
      781 atttatctgg caacttttta ttctgaaaca cgcaaaatga cgccaataac tggcggcttt
      841 gccaataaga cacagaactt tgaagcggtc gctcaatacc agtttgactt tggtctgcgt
      901 ccatcgctgg gttatgtctt atcgaaaggg aaagatattg aaggtatcgg tgatgaagat
      961 ctggtcaatt atatcgatgt cggggctaca tattatttca acaaaaatat gtcagcgttt
     1021 gttgattata aaatcaacca actggatagc gataacaaat tgaatattaa taatgatgat
     1081 attgtcgcgg ttggcatgac ctatcagttt taatgattat tgccggatgt gatgcatccg
     1141 gcacatttca ctcacgccgt taacttcacc ggctcgtcac gaaaatcatc cgccggttcc
     1201 agcttcagat tcagcgtcgt cagcagttca cgcagcttct cgtgaaactc acgcagggtg
     1261 tgctccagtc gttcaaccac ttcagtgtct tttaccggaa cactcttcca gtcgcctgct
     1321 ttatcgaaca gaccaaactg gtaactgtag gtaaaacggg attcctgcgc ttcaagctcc
     1381 atccaccagc cccagaactc acgcacttcc ggtgccggtt tcacgttgac gcatacagcc
     1441 agacaatcga aaaagaatcg attatcttcg cacttacctt cacgaatata cgggcctagt
     1501 gcggtaaatt ttttgatcaa tctgctcttc gggtgtccac tcggtaacgt cattgctatc
     1561 tcctgttgtg atgcaactgt tttaccaaat tggcaaaatt tagcaaattt ttaacacaag
     1621 cgtttttcga tccagccggt gatttcctga agacctttgt caaaattccg atacaccggg
     1681 ttaaatggga tctctaataa tttaccgtca gcagatgatg aggtgattaa gcgtgagtcc
     1741 tcttccgggc tgaacggatc gttcttccag tagcctgata acattggcgt tgggcagcga
     1801 cgccccagca atccttgcac ttttaatgaa tagcgattca gctccacgcg taacgcgtca
     1861 tcggaagcat catgcatccc caaacgactc gccagaacgt caagatacat ttccggcacc
     1921 tgttgctggc acttaaaatc actcaacagg gtatgaacta ccggaccaag acaggcaacc
     1981 gctttcagac gcggcgattc aagatatgcc agacgcacgg caacgttagc gccgaaacgg
     2041 aaaccaaagg ccgcgacgcg agtgtgatcc acccacggta cgttaggcag cgcctttaag
     2101 acgtgctgat gcaacaggct ggagtcctgg gtgagcttcc attttgaaga aaagcccacc
     2161 gatggcatat caatagtcag catcgcaatg ccgcgcggcg caaaataacg ttcatacagg
     2221 ctgtaatagt ccgtctgcat cgcatccaga ccaccacaca ttaatactgt cgggaacggg
     2281 ccatcgcctt tcggcatatg caaaaagccg gtgatgggcg caccgccggg tacggtaaac
     2341 tccatctgcc gcatcgtgcc cggcagacgc tgggcggcct cttcataggc gcggtttgat
     2401 aaagcctgcg cttgctcggc caggtcatct cctttcagat gaggataggc ggcaatgttg
     2461 tacaacgtag ccgcatgcag ccagtgtcga ccactgagtt gcggatcttc ttccgcacag
     2521 gctttttgtt gccacaccat cgcctgggtg gcccactcat aaatccagtt gccaccacgg
     2581 tagccaatca cagtatcgta taaatcatcg tcggtacgtt cagcatcgct catcacaata
     2641 cgtgcctgga cgtcgaggat ttcacgtggg tcaataccgc gccagatcca catcagacgg
     2701 ttaatcatgc gataccagtg agggatggtt ttaccatcaa gagccgactg cacaggcggt
     2761 tgtgcgccgt gattaaagcg gcggactagc gtcgaggttt caggatgttt aaagcggggt
     2821 ttgaacaggg tttcgctcag gtttgcctgt gtcatggatg cagcctccag aatacttact
     2881 ggaaactatt gtaacctgcc tgaagttaaa aagaacaacg cccggcagtg ccaggcgttg
     2941 aaaagattag cgaccggaga ttggcgggac gaatacgacg cccatatccc acggctgttc
     3001 gatccaggta tcttgcggga tatcaacaac atagtcatca accagcggac gaccagccgg
     3061 ttttgcgaag atggtgacaa agtgcgcttt tggatacatt tcacgaatcg caacagcagt
     3121 accaccggta tccaccaggt catcaataac gatgaagcct tcgccatcgc cttctgcgcg
     3181 tttcagcact ttaagctcgc gctggttgtc gtgatcgtag ctggaaatac aaacggtatc
     3241 gacatgacga atacccagtt cacgcgccag taacgcaccc ggtaccagac cgccacggct
     3301 tacggcaata atgcctttcc attgttcaga aggcatcagt cggcttgcga gtttacgtgc
     3361 atggatctgc aacatgtccc aggtgacgat gtatttttcg ctcatgtgaa gtgtcccagc
     3421 ctgtttatct acggcttaaa aagtgttcga ggggaaaata ggttgcgcga gattatagag
     3481 atctggcgca ctaaaaacca gtatttcaca tgagtccgcg tctttttacg cactgcctct
     3541 ccctgacgcg ggataaagtg gtattctcaa acatatctcg caagcctgtc ttgtgttgac
     3601 aacattttct gctaaccctg tgacctgcaa tactgttttg cgggtgatcg acaaggagac
     3661 ttaacgtgtc tgaactgtct caattatctc cacagccgct gtgggatatt tttgccaaaa
     3721 tctgttctat tcctcacccg tcctatcatg aagagcaact cgctgaatac attgttggtt
     3781 gggcaaaaga gaaaggtttc catgtcgaac gcgatcaggt aggtaatatc ctgattcgta
     3841 aacctgctac cgcaggtatg gaaaatcgta aaccggtcgt cttacaggcc cacctcgata
     3901 tggtgccgca gaaaaataac gacaccgtgc atgacttcac gaaagatcct atccagcctt
     3961 atattgatgg cgaatgggtt aaagcgcgcg gcaccacgct gggtgcagat aacggcattg
     4021 gtatggcgtc tgcgctggcg gttctggctg acgaaaacgt ggttcacggc ccgctggaag
     4081 tgctgctgac catgaccgaa gaagccggta tggacggtgc gttcggctta cagagcaact
     4141 ggttgcaggc tgatattctg attaacaccg actccgaaga agaaggtgaa atctacatgg
     4201 gttgtgcggg gggtatcgac ttcacctcca acctgcattt agatcgtgaa gcggttccag
     4261 ctggttttga aaccttcaag ttaaccttaa aaggtctgaa aggcggtcac tccggcggtg
     4321 aaatccacgt tggcctgggt aatgccaaca aactactggt gcgcttcctg gcgggtcatg
     4381 cggaagaact ggatctgcgc cttatcgatt tcaacggcgg cacactgcgt aacgccatcc
     4441 cgcgtgaagc ctttgcgacc attgctgtcg cagctgataa agtcgacgtc ctgaaatctc
     4501 tggtgaatac ctatcaggag atcctgaaaa acgagctggc agagaaagag aaaaatctgg
     4561 ccttgttgct ggactctgta gcgaacgata aagctgccct gattgcgaaa tctcgcgata
     4621 cctttattcg tctgctgaac gccaccccga acggtgtgat ccgcaattcc gacgtggcaa
     4681 aaggtgtggt cgaaacctcc ctgaacgtcg gtgtggtgac catgactgac aataacgtag
     4741 aaattcactg cctgatccgt tcactgatcg acagcggtaa agactacgtg gtgagcatgc
     4801 tggattcgct gggtaaacta gctggcgcga aaaccgaagc gaaaggcgca tatcctggct
     4861 ggcagccgga cgctaattct ccggtgatgc atctggtacg tgaaacctat cagcgtctgt
     4921 tcaacaaaac accgaacatc cagattatcc acgcgggcct ggaatgtggt ctgttcaaaa
     4981 aaccgtatcc ggaaatggac atggtttcta tcgggccaac tatcaccggt ccacactctc
     5041 cggatgagca agttcacatc gaaagcgtag gtcattactg gacgctgctg actgaactgc
     5101 tgaaagaaat tccggcgaag taattatttg atttgctgcc ggatggcgtt taatcgcctt
     5161 ccggcagttt catccttcat tatccttcga taaaagccat ccctgtaaat gtccgtctcg
     5221 ggttgccacg ttcaatctgg tgatggaaca ttcgccgctg cgatttcagc accgcgctat
     5281 tttcctgttg ctgctgctcc agcttccagg caatcagcaa tcgtgccagc cgcttgttag
     5341 catgctgact acgctctgac tgaaccttca cgctaatacc ggatgccaaa tgcgtggcgc
     5401 gtaccgccga gtcggtttta ttgacatgtt gaccgcccgg ccccgacgaa cgcaacgtct
     5461 cataacggat tgcatccgat tgttcctgct catcagcggt aaaacgccca atgcccagaa
     5521 accagttttt gcgcccatga tgaggccgat acggactcgg acaaatccac tgaatagtgc
     5581 cgcaccagct ttcgcttaat gcccatgcgt tatcgccatc cagagaaacc agagctgaac
     5641 gcagcgtgtc agagtagcgg cccgtttctg tttccaacac cgttaccgcg acgtcttgtc
     5701 gggcagcttc tttaattaat cgctccagcg cctttttcac tgcgagacaa cattcttccg
     5761 gcccctgcgc tgaggagagt tgtagcaaga tcatcctttt ttccctccac tgtttttgag
     5821 cgttagcacc ggacgcagcc gcgcgacagg aataattaaa ccggcctgca ccagacattg
     5881 caccacgctt tcagccgatt tataggcttg tggcgcttct tcaaagatga gttgtttatc
     5941 gcgacaaatt acccgactgc caagttcagt ccgcgagagt tgcgtcaccg tgtatttcgc
     6001 tgccagacgc cctttacact cggtgcgccc ccatttacgc cctgccccat gcgccagcga
     6061 gtggagcgtt ttttcgttcg caacaggctg tacaatccag gagtaatcac cacgtgaacc
     6121 gggaataatc accagaccgt tgtcatccgg cgtggcacct ttacgatgca accagccctg
     6181 ttgatcgccg atttgacacg cgctaacaaa gttatgcgcc acatccagaa ccggacttcc
     6241 cgtggcctta acttgttgca ttatgcgcaa agcgatcatc tggcgattaa tacacgcaaa
     6301 cgccagcgca tcgtcatgtt ccgcaatata ccgtagcgcg tcgtcactgc cttgaggcaa
     6361 accatgatgc gagaacgagg caatatgccg ctgtaaaata gactgcccca ccccccgcga
     6421 gccgctatga accaacagtt gcagatgttg tgcatccaga cctgccagtg caaacagttc
     6481 agcgttgatg atttgatcaa cttgttgcag ttcagcgaaa tgattgccgc caccgataga
     6541 gccaagcgag ctgcgccacg gatgctgtgc aaatgctgac ggcaggtttt cctccagcca
     6601 gctttcttca gcaacgtcat ccagatctga taatcgcttt tcaaacttat cggcgttgta
     6661 tttgcgagtg agaatatctg tttgccatag cgccataccg cagccgatat cattgccgac
     6721 cagtgccgga taaaaacgac caacagagaa gaacgctgcg ccaatcggat agccgcgtcc
     6781 gggatgtaaa tctggcatcc ccactacgcg ctgcatgttg ggtaaatttg ccgtggtgtg
     6841 taattgttgg atcgctaaac tctcgatcca caggtcatca gatgcgatgg taaataccgc
     6901 atcggataag ggacgaatat aattgcccat ataccattcc gtaaatagac tcgtcatact
     6961 tcaagttgca tgtgttgcga cttgaattat tgagagtaac aattaaaaaa tcaggaaatg
     7021 gcagacgaat ccagggcaag aaaaaaagaa ttaatcctgg aaaggtctgc aaagagtcat
     7081 tgataaagga tttgtcatga tttacctcct tgcagtttgt ggaataagaa aacgcgcaca
     7141 tactaggctt gcgttatttt cattgcaagc tggatttaat gttgctgttt ataacgcata
     7201 taaaaattaa taaaccactc tccccgacat tcaacgcgtt gctgcttaac tgtctgaaaa
     7261 ccataacgtt caaaaaaggg ttttgcggtt atgcttgcgt ccaccgtaag ttcggattca
     7321 gacttaatca aaggttttaa caaagcgctg gcaaccccac ggcgggtgta ttcagggtca
     7381 acaaataaca tatcgatata gtgttcaacg caggtaataa agccgaccag ttttgcatta
     7441 atgactgcaa cccgcacttg tgatttcgcg agtttctcct tccagcgaga ttcgtcaatc
     7501 tgcgcccagg cggcaatttg ttgtggtgaa taatgctgac tggcagtcat cgtaaccgct
     7561 ctaatgaaaa tagcgcatag ttgctgaaaa tcgccaggct gatagtctct tatttgtatg
     7621 ttattcataa tcccagcacc agttgtcttt ccatttgcgg gtcaagcaac gtcacatgca
     7681 gccccaccag tcgcacaccg cgcccgccgc ggcgttcatc ccaggtttta cgcgcggtgg
     7741 cgattaagtc agctttattc agccgcggcc agacgtgctc ctgagtggtt tgctgaaaat
     7801 cgtcgaactt taatttcacc ccctggcggg caatcagtaa atcaggtttt acctttgcca
     7861 gacggcgttc aagttccgga tacagccgct cgataatcgc ttcacattca gaccagtggt
     7921 ggatatcttc cgccatcgtg cgttccacgc cgacggattt tcgcaaccgt tcgctgttaa
     7981 cgtcgcgctc gtcaatcccc tgactacgct cccacaaaat gcggccaaat ttgccaaagc
     8041 gtttaagcag catcaccaga tcacactttt gtacatcacc gcaggtccgc agccccatcg
     8101 cttccagttt tgccgccgag actttgccga cgccggggat ttttgccagc ggtaaggttt
     8161 gtaaaaatgc cggaacttct gccggcgtaa tcacaaactg accgttgggt ttattcatgt
     8221 cggaggcgat tttggcgaga aactttactg gtgccacgcc cgcagatgcc gtcagttgca
     8281 gctcgttgaa aatcgtctgg cggatttcct gggcgatgag agtcgcagaa ccgtggcaat
     8341 ggacgctatc ggtgacatcg agataggctt catccagtga caacggttca atgcgcgagg
     8401 tgtagcgcga gaaaatttcg cgaatatgat ttgaggcttc tttgtaggcg tcaaagcgcc
     8461 ccggaagcaa ggtgagatgc gggcataatt tgagcgccat ccctgtcggc atagcgctac
     8521 gtacgccaaa tttacgcgcg ggataattgg cggtgctgat caccccccga cgttcgcggc
     8581 tgccgccaat agcaataggg atatcgcgca gggcgggatt gtcgcgcatc tccaccgctg
     8641 cgaaaaagca gtccatatcc acatgaatga ttttacgcat tgctcacctc tcaacactgg
     8701 taaagtatac agtgatttca gggtttgaga aatgcgtaaa gattcagcat ctagcgcgtt
     8761 cgctgtgaaa gcacctgctg cttatccagc ttttgcacca gcggctgcac cactttatcc
     8821 ccatgctgac caaagtattg atacagcgta tcggacgcac ttttggtcag caccataatc
     8881 tcaatgcgcc ggttgcccgc gctttgcgga tttttcgcgt ccagcagcat ctggtccgcc
     8941 attgcgctta cctgcatcac tttatcttcc ggcattccgg cctcttccag cacccgacga
     9001 gctgaaagcg cgcgatcgcc cgaaaggttc cagttgttgt agatattgtt tttgtaggcc
     9061 atcgcatcgg tatgcccggt aataataatt ttgttatcga gcgagtcgaa aactggcgcg
     9121 agctccacca gcagcctttt aaagaacggc ataatctgcg cgctgccgcg ttcgaacata
     9181 ttgcgattct ggtcgtcttt aatcagcacg cgtaatccct gcgggacaat ctccatctcc
     9241 agattcgctt ccatatgcgc atcacgggca atggtgttga tactggttgc cagctcgcca
     9301 agctcggtgg cggatttctt cttcagcagc accgttttct cattgacgtc acgcgctttt
     9361 ttctccgtct cttccggcac cgccacggtg gcaggttttg gcggatgaga cgggctgatt
     9421 ttatttaacg gcgacagccc accaccgtta aaaatcgact gcccgtgcag cgcggcgata
     9481 atgctttcac gctcggattt actgacgctg ttgacaatca actttattgt cgtcaccaaa
     9541 ggggccgagc gtatttccga ggtttcagcc cgctttaccc tggatgcgat gcccggtaag
     9601 cagatggcga ttgacgccga tcttaatgcc gggttgatca atcaggcgca ggcacaaacc
     9661 cgacgtaaag atgttgccag cgaggccgat ttctacggcg cgatggacgg ggcatcgaaa
     9721 tttgtgcgcg gggatgctat cgccgggatg atgattctgg cgatcaacct gatcggcggc
     9781 gtctgtatcg ggatcttcaa atacaacctg agcgccgacg ccgccttcca gcagtatgtg
     9841 ctgatgacca tcggcgacgg gctggtggcg caaatccctt ccctgctact ctccaccgcg
     9901 gcggcgatta tcgtcacccg cgtcagcgac aacggcgata tcgcccatga cgtgcgtcac
     9961 cagttgctgg caagcccatc ggtgctctac accgctaccg gaattatgtt cgtactggcg
    10021 gtagtgcctg gaatgccaca tctgccgttt ttgctgttca gcgccctgct tggctttacc
    10081 ggctggcgga tgagcaaacg cccgcaggcg gcggaagcgg aagagaaaag tctcgaaacg
    10141 ctgacccgca ccatcaccga aaccagcgag caacaggtca gttgggaaac cattccgctg
    10201 atcgagccca tcagcttaag cctcggctac aagctggtgg cgctggtgga caaagcccag
    10261 ggcaacccgc tcacccagcg tattcgcggc gtgcgacagg tgatatccgc cggcaacggc
    10321 gtgctgctgc cggagatccg cattcgggaa aacttccgcc ttaagcccag ccagtacgct
    10381 attttcatca acggcattaa ggctgatgaa gcggatattc cggcggataa actgatggcg
    10441 ctgccctcca gcgaaaccta cggcgagata gacggcgtgc tggggaacga cccggcgtac
    10501 gggatgccgg tgacctggat ccagcctgcg cagaaagcga aggcgctgaa tatggggtat
    10561 caggtgatcg acagcgccag cgtgattgct acccatgtaa ataagattgt gcgcagctat
    10621 attcctgatt tatttaacta tgacgatatt acgcagttgc ataaccgttt ggcgtcgatg
    10681 gcaccgcgcc tggcggagga tttaagcgcg gtgctcaatt acagccagtt gctgaaagtg
    10741 taccgggcgc tgctgaccga aggcgtttcc ctgcgcgata tcgtcaccat cgccaccgtg
    10801 ctggtcgcca gtagcgcggt gaccaaagat catattctgc tggcagccga tgtgcgcctg
    10861 gcgctgcggc gcagcattac ccatccgttc gttcgcaagc aggagctgac ggtgtatacc
    10921 ctgaataatg agctggaaaa tctgctgact aacctagtga atcaggcgca acagggcggt
    10981 aaagtgatgc tcgacagcgt gccagtggac ccgaatatgc tcaaccagtt ccagagtacg
    11041 atgccacagg tgaaagagca gatgaaagcg gcggggaaag acccggtgct gctggtgcca
    11101 ccgcagctgc gccctttgct ggcgcgttat gcaaggttgt ttgcgccggg gctgcatgtg
    11161 ctgtcgtata acgaagtgcc ggatgagctg gagttgaaga ttatgggggc gttgagttga
    11221 tgggggattc ttttccatgc tttgcagcac ctgacaccat taattgacgt cacactgcaa
    11281 agagactacg atgacaccat acaatgacgt catggattga tagtatgatt aaggtcagcg
    11341 cgctaggttc tcgtcataga aaaacattcg acgatgtaat gacggagccg ccaaaatctg
    11401 gaattaaatg ggatgatgtt gttgcactca ttaaagctgt gggtggaaca ataaaaaata
    11461 acaacggctc cagacgaaaa tttcagattg gaacgaccaa attcagcaca catgagccac
    11521 atccaaagaa tgtgatggat aaaggcgcag tagcaggtct taaggagtgg tttgttaact
    11581 gtgtaggggt agattatgaa taaagcatcg aacattatca caattaaagg tcaaccagcc
    11641 gcagtgacat ttgaagccga tatcaacgca tttcgcggca agttcctcaa tgtaaacggt
    11701 tattgtgact ttgtagccac aagtattgag gcattatatc gtgaaggcga aagcgcgctg
    11761 gatgagtgga tggcggattg tgaagaggac ggtattgccc cttttcgtga agaagaagag
    11821 caaaaacgcc tgacgcttcg tgttcctcat cacattgatt cgcggctgac catcgtcgcc
    11881 agacaacatt ccatttcaaa aaaccagctc attgttgatg tcttagaacg tgagttttca
    11941 gcccagatgt aaacaggtcg gatgccgcgt ggcatccgac cttttgccag ttcgtcaata
    12001 ttacccattt acaaaatcgt ctcttccgtc aatatccgcc gcgcgcctaa aaagtgatcc
    12061 tgccagaaag gttcagctaa ccgacttacc cgaatggttt ccccggtgcg cggtgactca
    12121 ataaactgac cgtcgcccag atacaccccc atatggtcgg caatctcgcg gctgtggata
    12181 tggaaaaata gcaaatctcc ccggcgcagg tcgttattcg ccacaatggt tgcccggcga
    12241 tagtgataca tctcattagc cgtgcgtggg agtttcgcct caaggatctt gttgtaggca
    12301 taaaaaacta acccgctaca gtcgaagcct tgatcgggcc gcgtaccgcc ccagacgtaa
    12361 ggcttgccaa gctgttgctc cagacggtga atcgccactt cggtgatgtt atgcagatgg
    12421 tcgctgctta agaaatggtt gttttccgcc agcgcctgcc agcgtctgtc actcgccttg
    12481 agcggcgcag gaaaccattc tggatgctgt tttatctgtt cgcggttgtg ctggcgtagc
    12541 gcccttctgc tttcggcatt gccttccaca atatagctgg cctgtttttt cagatgggtt
    12601 tggtactgtt ttaataaacg cgcacgattt tgcatccgct ggcgggcggc gtagctgaaa
    12661 gtgatatgtg atgtggtggc gctggcaaat gaagaaaacg gcaagcaaag caacagtaat
    12721 cccgacaaca caaaagaggg aatcgacggc aaggacatac tcaataatgg ctgatcatgc
    12781 attgatgttg cagtgtatcc cggcccgtac ctg
//
LOCUS       DAPGWD010000119        12701 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPGWD010000119 DAPGWD010000000
VERSION     DAPGWD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.120"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            90..1604
                     /gene="caiT"
                     /locus_tag="RHX88_003286"
     CDS             90..1604
                     /gene="caiT"
                     /locus_tag="RHX88_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDV2231947.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1635..2777
                     /gene="caiA"
                     /locus_tag="RHX88_003287"
     CDS             1635..2777
                     /gene="caiA"
                     /locus_tag="RHX88_003287"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDV2231948.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2905..4122
                     /gene="caiB"
                     /locus_tag="RHX88_003288"
     CDS             2905..4122
                     /gene="caiB"
                     /locus_tag="RHX88_003288"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDV2231949.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLSTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4196..5749
                     /gene="caiC"
                     /locus_tag="RHX88_003289"
     CDS             4196..5749
                     /gene="caiC"
                     /locus_tag="RHX88_003289"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDV2231950.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVVNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5858..6643
                     /gene="caiD"
                     /locus_tag="RHX88_003290"
     CDS             5858..6643
                     /gene="caiD"
                     /locus_tag="RHX88_003290"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDV2231951.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6649..7239
                     /gene="caiE"
                     /locus_tag="RHX88_003291"
     CDS             6649..7239
                     /gene="caiE"
                     /locus_tag="RHX88_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDV2231952.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7325..7720)
                     /gene="caiF"
                     /locus_tag="RHX88_003292"
     CDS             complement(7325..7720)
                     /gene="caiF"
                     /locus_tag="RHX88_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDV2231953.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7981..11202)
                     /gene="carB"
                     /locus_tag="RHX88_003293"
     CDS             complement(7981..11202)
                     /gene="carB"
                     /locus_tag="RHX88_003293"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDV2231954.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11220..12368)
                     /gene="carA"
                     /locus_tag="RHX88_003294"
     CDS             complement(11220..12368)
                     /gene="carA"
                     /locus_tag="RHX88_003294"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDV2231955.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2983 a         3292 c         3369 g         3057 t
ORIGIN      
        1 gaggttaata ataattatat caaatgttaa caaaaataaa acaaacggga acgcaaaata
       61 aatattcgtt ttcacagtgg aattaactca tgaagaatga aaagagaaaa acgggaatag
      121 aaccgaaggt tttctttccg ccgttaataa tcgtcggcat actttgttgg cttacagtca
      181 gagatctgga tgcagcgaat gtcgttatta atgctgtatt cagttacgtc accaatgtat
      241 ggggatgggc atttgaatgg tatatggtgg tgatgctttt cggttggttc tggctggtgt
      301 ttggcccgta tgccaaaaag cgtttaggta acgaaccacc tgaatttagt accgccagtt
      361 ggatctttat gatgttcgcc tcctgtacgt ctgctgccgt actgttctgg ggatcgattg
      421 agatctacta ctacatctcc accccgccgt ttggcttaga accgaactcg acaggggcga
      481 aagagttggg gctggcttac agcttgttcc actggggacc tctgccgtgg gccacttaca
      541 gcttcctttc agtcgccttc gcttacttct tctttgtccg caaaatggaa gtgattcgcc
      601 ccagctcgac actggtgccg ctggtaggtg aaaaacacgc caaagggttg ttcggcacta
      661 tcgtcgacaa cttctatctc gtcgccttga tcttcgcgat gggtaccagt ctgggccttg
      721 ccacgccgct ggtgaccgag tgtatgcaat ggttgtttgg cattccgcat accctgcaac
      781 tggacgctat catcattacc tgctggatta tcctcaacgc catttgcgtc gcttgcggtc
      841 tgcaaaaagg ggtacgtatc gccagtgacg tgcgtagtta cctgagcttc ctgatgctgg
      901 gttgggtgtt cattgtcagc ggtgccagct tcatcatgaa ctacttcacc gattcggtgg
      961 ggatgttgct gatgtatctg ccgcgcatgt tgttctatac cgatcccatc gctaaaggcg
     1021 gcttcccgca gggctggacc gtgttctact gggcatggtg ggtgatttat gctatccaga
     1081 tgagtatctt cctcgcccgc atctcccgtg gtcgtactgt gcgtgaactg tgcttcggca
     1141 tggtgctggg gctgacagcg tcaacctgga tcctgtggac tgtactcggt agtaacactc
     1201 tgctgttgat agataaaaac atcatcaaca ttccaaatct gatcgaacag tacggtgtgg
     1261 cgcgcgccat cattgaaacc tgggccgctc tgccactcag caccgccacc atgtggggct
     1321 tcttcatcct ctgctttatt gccaccgtta cgctggttaa cgcctgctct tataccctgg
     1381 cgatgtccac ttgccgcgaa gtacgcgatg gtgaagaacc acctctgctg gtgcgtatcg
     1441 gttggtcaat tctggttggc attatcggta ttgttctgct ggcgctcggc ggcctgaaac
     1501 cgattcaaac cgccattatc gccggaggat gcccgctgtt cttcgtcaac attatggtga
     1561 cgctctcctt tattaaagac gcgaaacaga actggaaaga ttaattaacc cccaaaatat
     1621 caagaggttg aaagatggat tttaatttaa atgatgagca ggaactgttt gtcgccggta
     1681 tccgcgaact gatggccagc gaaaactggg aggcctattt tgccgagtgc gaccgtgaca
     1741 gcgtctaccc ggaacgtttt gtcaaagcac tggcggatat gggtatcgac agtctgctga
     1801 tccctgaaga gcacggtggt ctggacgcgg ggtttgttac tctcgccgcc gtgtggatgg
     1861 agctgggacg tctgggggca ccaacctatg tgctgtacca gttgccgggc gggttcaaca
     1921 ccttcctgcg cgaaggcaca caagagcaga tcgacaagat tatggctttc cgcggcaccg
     1981 gtaagcagat gtggaactca gcgattaccg aaccgggcgc gggctccgac gtgggtagcc
     2041 tgaaaacgac ttatacccgt agaaatggta agatttatct taatggtagt aagtgtttta
     2101 ttaccagcag cgcctacacc ccgtacatcg tggtgatggc gcgcgacggg gcttctccgg
     2161 acaaacctgt ctacaccgaa tggtttgttg atatgagcaa accgggcatc aaagtgacca
     2221 aacttgaaaa gctcggtctg cgtatggata gctgctgtga aatcaccttt gacgacgtgg
     2281 aactggacga gaaagacatg ttcggtcggg aaggtaacgg ctttaaccgc gtcaaagaag
     2341 agttcgatca tgaacgtttc ctggtagctc tcaccaacta cggtacggcg atgtgcgcct
     2401 ttgaagatgc ggcgcgctac gccaaccagc gcgtgcagtt tggcgaggct attggccgtt
     2461 tccagttgat tcaggaaaaa ttcgcccaca tggcgatcaa attaaactcc atgaaaaaca
     2521 tgctgtatga agcagcgtgg aaagcagaca acggcaccat cacctctggc gatgcagcga
     2581 tgtgcaaata cttctgcgcc aatgcggcat ttgaagtggt ggatagcgca atgcaggtgc
     2641 tgggcggtgt cgggattgcg ggcaaccacc gcatcagccg cttctggcgt gacctgcgtg
     2701 tagaccgcgt ctccggggga tctgacgaaa tgcagatcct gacgctgggt cgcgcggtgc
     2761 tgaagcaata ccgctaagtc cgtgcgctgc ctgatgcgac gctaatgcgt cttatcaggc
     2821 ctacgggagc acgaatgtag gccggataaa gcgtttacgc cgcatccggc agtcatgcgc
     2881 tacataattt ttcaggagct aataatggat catctaccca tgccgaaatt cgggccgttg
     2941 gccggattgc gcgttgtctt ctccggtatc gaaatcgccg ggccgttcgc cgggcaaatg
     3001 ttcgcagaat ggggtgcgga agttatctgg atagagaacg tcgcctgggc cgacaccatt
     3061 cgcgttcaac cgaactaccc gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat
     3121 attttcaaag atgaaggccg cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc
     3181 atcgaagcca gtaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg
     3241 cagcacaacc cgaaactggt tatcgctcac ctgtccggtt ttggtcagta cggcaccgag
     3301 gagtacacca atcttccggc ctataacact atcgcccagg cctttagtgg ttacctgatt
     3361 cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga ttacttttct
     3421 ggcctgaccg ccaccacggc ggcgctggca gcactgcata aagtgcgtga aaccggtaaa
     3481 ggcgaaagta tcgacatcgc catgtatgaa gtgatgctgc gtatgggcca gtacttcatg
     3541 atggattact tcaacggcgg cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac
     3601 tacgccggtt gcggtctgta taaatgtgcc gacggctaca tcgtgatgga actggtgggc
     3661 attacccaaa ttgaagagtg ctttaaagat attggcctcg cacatctact cagtacgccg
     3721 gaaatcccgg aaggcactca gcttatccac cgtatcgaat gcccttacgg cccactggta
     3781 gaagaaaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa agaacgcttt
     3841 gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga aagcaatcca
     3901 cagtatgttg cccgtgaatc aatcactcag tggcaaacga tggatggtcg cacctgcaaa
     3961 gggccgaaca tcatgccgaa attcaaaaat aaccccggac aaatctggcg cggaatgccc
     4021 tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga aaacgacatt
     4081 caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg
     4141 cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa atggatagag gtgcaatgga
     4201 tatcattggc ggacaacatc tacgtcaaat gtgggacgat cttgcggacg tttacggtca
     4261 taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt aaccggtata gttatcttga
     4321 gttaaatcag gagattaacc gcacggcaaa cctgttttat acgctgggga ttcgcaaagg
     4381 cgacaaggtt gcactacatc tcgacaactg cccggaattt atcttttgct ggttcgggct
     4441 ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc ctgttacgcg aggaaagcgc
     4501 gtggatcctg caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat tctatcctat
     4561 gtatcaacag attcagcagg aagatgccac tcaattgcgg cacatttgcc tgacagatgt
     4621 ggcacttccc gctgatgatg gcgtgagttc gtttactcaa ctgaaaaatc aacaacctgc
     4681 caccttgtgc tatgcaccgc cgctatcgac tgacgatacg gcggaaattc tcttcacctc
     4741 cggcaccacc tcccgaccga aaggtgtggt gattacccat tacaacctgc gcttcgctgg
     4801 atattactcc gcctggcagt gtgcactgcg tgacgatgac gtctacctga cggtaatgcc
     4861 tgcgtttcat atcgattgcc agtgtactgc ggcgatggcg gcgttttctg ccggggccac
     4921 ctttgtgctg gtcgagaaat acagcgcccg cgccttctgg ggacaggtac agaagtaccg
     4981 cgccaccgtt accgaatgta ttccgatgat gatccgtacg ttgatggtgc agccgccttc
     5041 agcgaacgat cggcaacacc gcctgcggga agtgatgttt tatctcaact tgtcggagca
     5101 ggaaaaagat gcgttttgtg aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac
     5161 ggaaaccatt gttggcatta tcggcgatcg ccctggcgat aaacgacgct ggccgtcgat
     5221 tggtcgggcg gggttttgct acgaagcgga gatccgcgac gatcacaatc gcccgctccc
     5281 ggcaggtgag atcggtgaaa tctgtattaa aggcgtacca gggaaaacca tcttcaaaga
     5341 gtattttctc aacccgaaag ccactgcgaa agtgctggaa gccgacggct ggctacatac
     5401 cggcgatacc ggatactgcg acgaagaggg ctttttttat ttcgtcgatc gccgctgcaa
     5461 tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag ctggaaaata tcatcgccac
     5521 ccatccaaaa attcaggata tcgtggttgt gggtattaaa gattcgattc gcgatgaagc
     5581 catcaaagca tttgtggtgg tgaatgaagg tgaaacattg agcgaagagg aatttttccg
     5641 cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga tcagaaaaga
     5701 tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa tgaaacagca
     5761 gggaactacc ctgccagcta ataatcacgc tataaaacaa tatgcatttt ttgcagggat
     5821 gttgtcgtcc ctgaaaaagc aaaaatggag aaaaggaatg agtgaatcat tacatctgac
     5881 ccgcaatgga tcaattctgg aaattaccct tgatcgtcca aaagcgaatg ctattgatgc
     5941 aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg
     6001 tgtcgccatt attaccggtg ccggagagaa gttcttttcc gcgggctggg atttaaaagc
     6061 ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggtggatttg cgggattaac
     6121 cgaaattttc aatctcgaca aaccggttat cgcagctgtg aacggctatg cctttggcgg
     6181 cggtttcgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg ccagcttcgc
     6241 cctgccggaa gccaagctgg gcatcgttcc tgacagcggc ggcgtgctgc gtctaccgaa
     6301 gatcctgccg cctgccatcg tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga
     6361 agaggcgctg cgttggggga tagtcaaccg cgtggttagc caggcggaac tgatggataa
     6421 cgcccgcgaa ctggcgcagc agctggttaa cagcgccccg ctggcgattg cggcgctgaa
     6481 agagatcttc cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag
     6541 cggcgtgttg aaacactatc cgtcggttct gcattcggag gatgccattg aagggccgct
     6601 ggcgtttgcc gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt gagctattac
     6661 gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc
     6721 ttgattggcg atgtgattgt gggagccggt gtctacatcg gcccactcgc ctcactgcgt
     6781 ggtgactacg ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg
     6841 catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg gcacggagcg
     6901 atcctgcatg gttgtgtgat tggtcgcgat gcattggtcg ggatgaacag cgtgattatg
     6961 gatggcgcgg tcattggcga agagagcatt gttgccgcca tgagctttgt caaagcaggc
     7021 tttcacggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt
     7081 agtgacgacg agttacactg gaaacggttg aataccaaag agtatcagga tcttgtcggg
     7141 cgctgtcatg catcgttaca tgaaacacag ccactgaggc aaatggagga aaatcgcccc
     7201 cgtttgcagg ggacgacgga tgtgacgcct aaacggtgat cttgccggat gcgacctggt
     7261 tgctggataa gatgctacaa catcgcatct ggcaagcagg acgcccggta tcaacgagat
     7321 aaaattaacg acgcatactc tttgacaaca tcatctgcca cttctgctcg cggttaagtt
     7381 ccgtgagcgg aacggcagga atacgcggcg tctttcttac gccgactaat ttatcccggc
     7441 tgttattgcg caggcgcgcg taaatttgct catcgatatc gaccactttt accagtcgct
     7501 ggcactggca tccccgtcct tccagcttat tagggatcat cttaacttcg cagcttattt
     7561 ctgcgacttc cgacagaata taagtcaggg tattaaccgc cttgctgtgt tcaatatcga
     7621 aatgaataga gatctctctt gctatcaccc accgattttc agccatcatc cattcggcga
     7681 ttaacaagta gagtggtttt tcaacatatc cttcacacat acgaactcca gtttcacaaa
     7741 ttaaaagtct ggctatgact cgttttgaag caattaaaca aggccatatt aaaattgtgg
     7801 atatatcgat agtatgcaac ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca
     7861 tcaaaaatat cgattcccca aaagatttac ttaatgggca aaaaaaacgg tcctcatcag
     7921 agaaccgtat tacttattcg atcaaattag cggatgaaaa atatccgcca tgacacgcta
     7981 ttatttgatc tgcgcgtgca tttcctgcac cgaaattact ttttcggtcg cgtcggcatt
     8041 cagcgccatc gcggtagcga aaccaccgtt cagggtggtg tcataatgca ctttatattg
     8101 cagcgcactg cgacggatca cgcgggagtc ttcaatcgcg cgacggcctg aagtggtgtt
     8161 gatgatgtag gtatattcgc cattcttgat gcggtcctga atgtgcggac ggccttcatg
     8221 caccttgttc accagacgcg gattaatgcc cgcttcgccc agcacaatcg ccgtgccgtg
     8281 ggtcgcatcc agctcgaagc cctgtttcag cagttttgcc gccaggtcca ccacgcgttc
     8341 tttatcgcct tcgcgcacgg aaagcaacgc acgaccgtgt ttcttcatgg tggagttgct
     8401 gcccagctgc gctttggcaa acgcttcagc gaaggtgcgg cccacgccca tcacttcccc
     8461 ggtggagcgc atttctggcc ctaacagcgg gtcaacaccc gggaatttat tgaacggcag
     8521 caccacttct ttcaccgagt agtacggcgg gataacttct ttggttacgc cctgctcagc
     8581 cagcgatttg ccagccatca cacgcgccgc cacttttgcc agcggtacgc cggtggcttt
     8641 ggagacgaac ggaacggtac gcgccgcacg cgggttaact tcaatcaggt agacttcgtt
     8701 gtttttcacc gcaaactgca cgttcatcag accgcgcacc tgcaattcga aggccagttt
     8761 ctgcacctgc tggcgcatca catcctgaat ttcctgactt aaggtgtagg ctggcagaga
     8821 acatgcggag tcaccggagt gcacgcccgc ctgctcgatg tgctccatga tgccgccaat
     8881 cagcaccatt tcgccgtcgc agatggcatc cacgtcaact tctaccgcgt catcaaggaa
     8941 gtgatccagc aacactggcg catcgttaga cacgctgacc gccgtctgga agtagcgacg
     9001 caggtcagct tcgtcataga cgatttccat cgcccgaccg ccgagaacgt aagacggacg
     9061 taccaccagc gggtagccaa tctctttcgc cttctctacc gccatttcaa tagcggtaac
     9121 ggtggcgttc gccggttgtt tcagtttcag acgctcaacc gcatgctgga agcgttcacg
     9181 gtcttctgca cggtcgatag catccgggct ggtgccgata accggtacgc cagcagcttc
     9241 cagcgcgcgc gccagtttca gcggggtctg accgccgtac tgaacgataa cgcctttcgg
     9301 cttctcaata cgcacgattt ctagcacatc ttccagagtt accggctcga agtagaggcg
     9361 atcggaagtg tcgtagtcgg tggagacggt ttccgggtta cagttaacca taatggtttc
     9421 gtaaccgtct tcgcgcagcg ccagcgaggc gtgtacacag cagtagtcga attcgatacc
     9481 ctgaccgata cggttcgggc cgccgccgag aaccatgatt ttttcacggt cggtagacgg
     9541 attcgcttcg cactcttctt cataagtgga gtacatgtaa gcggtgtcgg tggcgaactc
     9601 tgccgcacag gtatccacgc gcttataaac cgggtgcagg tcatactggt cgcgcagctt
     9661 gcggatttcc gcttcgcgca cgcccgccag ttttgccaga cgcgcatcgg caaagccttt
     9721 gcgtttcagc tggcgcagga attcagcgtt caggccagtg atgcccactt ccgctacttt
     9781 ctcttccaga cgcaccagtt cttcaatctg taccaggaac caacggtcaa tattggtcag
     9841 gttgaacacg ccgtccacag acaggcccgc acggaaggca tcggcgatgt accagatacg
     9901 ctctgcgcca gcatctttca gttcgcgacg gattttggtt aacgcttccg ggtcatcgag
     9961 gctcactttc gggtcgaatc cagtcgcacc gacttccagg ccgcgcagcg ctttttgcag
    10021 ggattcctgc tgcgtgcgac caatcgccat cacttcgcca accgatttca tctgagtggt
    10081 cagacggtcg ttagcaccag cgaatttttc gaagttgaag cgaggaattt tagtaaccac
    10141 atagtcgatg gacggctcga aggaggccgg agtacgtccg ccagtgatgt cgttcatcag
    10201 ttcgtcgagg gtgtaaccca ccgccagttt cgccgccact ttagcaatcg ggaaaccggt
    10261 cgctttcgac gccagcgccg aagaacggga cacgcgtggg ttcatttcga taacaatcag
    10321 gcgaccgttt ttcgggttca ccgcaaactg gacattggaa ccaccggttt caacgccgat
    10381 ttcacgcagc accgccatcg aggcgttacg catgatttga tattctttgt cggtcagcgt
    10441 ttgggctggc gcgacagtga tggagtcacc ggtatggatg cccatcgcat caaagttttc
    10501 gatagagcag acgatgatgc agttgtcgtt tttatcacgc accacttcca tctcgtactc
    10561 tttccagccg atcagcgact catcaatcag cagctctttg gttggggaga gatccagacc
    10621 gcgggcgcaa atttcttcaa actcttcgcg gttataagcg ataccgccgc cgctaccgcc
    10681 catggtaaag gatgggcgaa taatgcacgg gaagcccacg tcagcggcaa ccgccagcgc
    10741 ttcttccatc gtgtgtgcga taccggaacg cgcggtttcc agaccaattt tcttcatcgc
    10801 tacgtcgaaa cgacggcggt cttctgcttt atcaatcgca tcggcggtcg caccaatcat
    10861 agtcacgccg aactcttcca acacgccctg acgctccagc tccagcgcgc agttcagcgc
    10921 cgtctgaccg cccatcgttg gcagcaccgc gtccgggcgc tctttttcaa taatcttgcg
    10981 taccacttcc cagtgaatcg gctcgatgta ggtcgcatcg gccatttccg ggtcggtcat
    11041 gatggtcgcc gggttggagt tcaccagaat gacccggtaa ccctcttcgc gcagggcttt
    11101 acatgcttgc gcgccagagt agtcaaactc acacgcctga ccgataacaa tcgggcccgc
    11161 acccagaatc aggatacttt ttatatctgt acgttttggc atggctcttt tactcctgat
    11221 tacttagcgg ttttacggta ctgctcaatt aactcgataa agtggtcgaa caacggcgcg
    11281 gcgtcgtgtg gaccagggct ggcttcaggg tgcccctgga agctgaacgc cggtttatcg
    11341 gtgcgatgaa tgccctgtaa cgtaccgtcg aacagggatt tatgcgtgac acgcaggttt
    11401 gctggtaatg ttgcttcgtc caccgcaaaa ccgtggttct gggcggtgat catcaccacg
    11461 tttttctcaa catctttaac cggatggttg ccgccgtggt gaccaaattt cattttgaca
    11521 gtcttcgcac cgctcgccag cgccagcagc tgatgaccga ggcagatgcc gaagaccgga
    11581 atatcggttt cgaggaattt ctggatggcg gtaatggcgt aatcgcacgg ggccgggtcg
    11641 ccaggaccgt tggagaggaa gatgccgtct ggattcattt tcagcacatc ttccgcagaa
    11701 gtttgcgccg gaacgatggt caggcgacag cctctgtcca ccaacatgcg caggatgttg
    11761 cgcttggcac caaaatcata agccacgacg tggaacggca gctcgtcttc ttttttcgct
    11821 tctggcaggc cgccggtcaa cgtccagctc ccttgtgtcc agctataggc ttctgcggtg
    11881 gtcacttctt ttgccagatc catgccattc agacctggga acgcgcgggc tttttctaac
    11941 gccaacgccg catccgggtt atcgcctgcg ataatgcagc cattctgtgc gcctttctcg
    12001 cgcagtaaac gtgtcagctt acgggtatcg atatcggcaa tcgccacgat gttatggcgc
    12061 ttcaggtaag aagagaggtc ttcggtatta cggaagttgc tggcaatcag cggcaggtcg
    12121 cgaatcacca gaccttgtgc atgtacctga gaagattctt catcggcgtc attagtgccg
    12181 acattgccaa tatggggata agtaagagta acgatttgac gagaatagga aggatcagtg
    12241 aggatttctt gataaccggt cattgaagta ttgaaaacga cttccccaac cgccgaacct
    12301 gttgccccta tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa tagcgctgac
    12361 ttaatcaaaa caccctccag agaatattca ctcactttat ttgcatatta attcacaatg
    12421 atgacgtaaa tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg cggcattctg
    12481 gagatatggg cgctaaaagt caacttaatg gcgggatttt ttatattatt ttgtggctca
    12541 tgcatgtttt acaggcatta agcggcaaaa agatcatata acctgttctg gaaaacgctt
    12601 gcgcagcaag aagattgcat gaaataaaag cgagtaggga aaagtggacc aaatggtcaa
    12661 aatttacatt aaaacaacaa aaaccgaaga caaaacatgt a
//
LOCUS       DAPGWD010000120        12569 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPGWD010000120 DAPGWD010000000
VERSION     DAPGWD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.121"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..825
                     /locus_tag="RHX88_003295"
     CDS             <1..825
                     /locus_tag="RHX88_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HDV2231956.1"
                     /translation="LPALSYAQVKNIVTGEYSAVSKIITEKETLYPSDKILLVFDIDN
                     TLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISMLYELSPMQLTEPQLPT
                     MLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRKKEDTLPPFEKGKLKR
                     SWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQANIHAMTKMFSKDKWSS
                     TDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLATLFPDRNKLCPIK"
     gene            complement(870..2762)
                     /gene="parE"
                     /locus_tag="RHX88_003296"
     CDS             complement(870..2762)
                     /gene="parE"
                     /locus_tag="RHX88_003296"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDV2231957.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(2791..3372)
                     /gene="yqiA"
                     /locus_tag="RHX88_003297"
     CDS             complement(2791..3372)
                     /gene="yqiA"
                     /locus_tag="RHX88_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDV2231958.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3372..4199)
                     /gene="cpdA"
                     /locus_tag="RHX88_003298"
     CDS             complement(3372..4199)
                     /gene="cpdA"
                     /locus_tag="RHX88_003298"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDV2231959.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4224..4646)
                     /locus_tag="RHX88_003299"
     CDS             complement(4224..4646)
                     /locus_tag="RHX88_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDV2231960.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(4647..5276)
                     /gene="nudF"
                     /locus_tag="RHX88_003300"
     CDS             complement(4647..5276)
                     /gene="nudF"
                     /locus_tag="RHX88_003300"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDV2231961.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5481..6962
                     /gene="tolC"
                     /locus_tag="RHX88_003301"
     CDS             5481..6962
                     /gene="tolC"
                     /locus_tag="RHX88_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDV2231962.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7110..7781
                     /locus_tag="RHX88_003302"
     CDS             7110..7781
                     /locus_tag="RHX88_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDV2231963.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            7787..8947
                     /locus_tag="RHX88_003303"
     CDS             7787..8947
                     /locus_tag="RHX88_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV2231964.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(8985..9773)
                     /gene="ygiD"
                     /locus_tag="RHX88_003304"
     CDS             complement(8985..9773)
                     /gene="ygiD"
                     /locus_tag="RHX88_003304"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDV2231965.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            9916..10689
                     /gene="zupT"
                     /locus_tag="RHX88_003305"
     CDS             9916..10689
                     /gene="zupT"
                     /locus_tag="RHX88_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDV2231966.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(10747..10917)
                     /locus_tag="RHX88_003306"
     CDS             complement(10747..10917)
                     /locus_tag="RHX88_003306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231967.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11178..11831)
                     /gene="ribB"
                     /locus_tag="RHX88_003307"
     CDS             complement(11178..11831)
                     /gene="ribB"
                     /locus_tag="RHX88_003307"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDV2231968.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(11935..12083)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12205..12495
                     /gene="ubiK"
                     /locus_tag="RHX88_003308"
     CDS             12205..12495
                     /gene="ubiK"
                     /locus_tag="RHX88_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDV2231969.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3205 a         3348 c         3051 g         2965 t
ORIGIN      
        1 ctgccagctt tatcttacgc acaggtaaaa aatatagtta ctggcgaata tagtgcggtc
       61 agtaaaatta tcaccgaaaa ggaaactctc tatccatccg ataagatatt attggttttt
      121 gacattgata atacgttatt aacgagtggc acacgcattg gcggtgatat ctggtatcaa
      181 tggcaaaccg ataaattgcc cttaaagccg gatgactcgc aaaaagttcc ctgcctgtat
      241 gaaaatacaa tcagtatgct ttacgaactt agccctatgc agcttaccga gccacagtta
      301 ccgacaatgt taaaacagtg gcaacaaaag cataccgctt ttgctctgac atcccgtgca
      361 ccggatactc tctttccgac attgcgggaa ttaaagcgaa acggcattga tttttcgtct
      421 tccgcactca ggaagaaaga ggatactttg ccaccctttg aaaaagggaa actcaaacgg
      481 tcatggctgt acagccaggg ggtatttttt tcttccgggc aagataaagg cgtaattctc
      541 gattatatgc tggataaaat ggggaacaaa tatgacgcga ttgtttttat cgatgacggg
      601 caagcaaaca ttcatgcgat gacgaagatg ttctctaagg ataaatggtc ctctacggat
      661 tttaccatta ttcactatac cagagtggag gatgagctta ttaaacaaca aggtgcagta
      721 ataacaactg cacaagcaga aaaaatggct acggactgga aaactctggc tggttcgctg
      781 gcaacactat ttccagatcg taacaaactg tgcccaataa aataatcagc gacaacccgg
      841 caaaacacat gtttgccggg taaagattat taaacctcaa tctccgccat gtcgcctttc
      901 tcttgcaacc agttgcggcg atcttccgag cgtttcttcg ccagcagcat atccatcatc
      961 gcgtcagtac gctgatcgtc ttcatcatcg atagtcaact gcaccagacg gcgagtgttc
     1021 ggatcaagcg tggtttcacg caattgcatc gggttcattt cccccagacc tttaaaacgc
     1081 tggacgttcg gcttgccttt cttgcgtttt aattgctcaa gtacgccctc tttctcttct
     1141 tccgtcagcg cgtaataaac ctctttcccg agatcaatac ggtagagcgg tggcagtgcg
     1201 acgtaaacgt gaccgtgttt caccaacgcg cggaagtgtt ttacgaacaa agcgcagagc
     1261 agcgtggcaa tgtgcagacc atcagagtcc gcatccgcga ggatacagat tttgccataa
     1321 cgaagctggc tcagatcgtc gctgtcagga tcgataccga tcgctaccga aatatcatgc
     1381 acttcctgcg aggccagcac ttcgtcggaa gaaacttccc aggtgttaag gatcttacct
     1441 ttcagtggca tgatcgcctg atattcgcga tcgcgcgcct gcttggcaga tccgcctgcg
     1501 gagtcacctt ccacaaggaa cagttcggta cggttaagat cctgcgcggt acagtcagcc
     1561 agtttgccag gcagtgctgg gccgctggtc agctttttgc gcaccacttt tttagccgca
     1621 cgcatacggc gctgggcgct ggaaatcgcc atctccgcca gcaactccgc cgcctgaacg
     1681 ttctggttca gccacaggat aaaggcatct ttcaccacgc cagaaacaaa tgccgcgcac
     1741 tgacgcgaag agagacgctc tttggtctgc ccggcaaact gcggatcctg catttttact
     1801 gacagcacat aggcgcagcg atcccagata tcttccgccg acaactttac accgcgcggc
     1861 agaatgttgc ggtattcaca gaactcacgc atcgcgtcca acaggccctg acgcagaccg
     1921 ttaacgtgcg taccgccctg catcgttggg ataaggttga cgtagctttc ggtcagcagt
     1981 tcaccgcctt ccggcagcca cagtagcgcc cagtccacag cttcagtatc gccagcgaaa
     2041 ttaccgataa acggtttttc tggcagcgtc ggtaaaccgt ttaccgcttc cgccaggtaa
     2101 tcattcagac cgtcctgata gcaccagcgc tgttcggtgt tgttgatctc atctttaaag
     2161 gtaatctcaa cgccggggca cagtactgct ttggccttca gcacatgcgt caggcgtgaa
     2221 acagaaaaac gcgggctgtc aaagaaggtt tcatccggcc agaagtgcac gctggtaccg
     2281 gtattgcgtt taccgcaagt gccgacaacc tgtaaatcct gcaccttttc gccattttca
     2341 aaggcgatgt tataaacctg accatcgcgg cgcacgttaa cttctacgcg cttcgacagg
     2401 gcgttaacca ccgaaatccc cacgccatgc aggccgccag agaactggta atttttgtta
     2461 gagaatttac cgcctgcatg cagacggcaa agaatcagtt caaccgccgg tacaccctct
     2521 tccgggtgaa tatccaccgg catcccgcgc ccatcgtcaa taacttctaa cgactggtca
     2581 gcatgtaaga ttacatccac gcgttttgcg tgacccgcca gtgcttcatc gacactgtta
     2641 tcaataactt cttgccccaa atggttaggg cgagtggtat cggtatacat ccccggacgg
     2701 cggcgaaccg gctcaagccc ggtgagtacc tcaatggcat cagcgttata agtttgcgtc
     2761 atggtttaag ttagcaattc gagttgatcg tcagagatgg tgcagaccaa gaaaatcgac
     2821 gatcgggttg aaataatctt cgaagcccgt gaatgcgtgg ttgccgcctt ctatgacagt
     2881 ctggcggcag gaagcgtagt acgccaccgc ctggcggtaa tccagcactt catctcccgt
     2941 ctgttgcagc agccagatca aatccggcgc ttccagcggg tcaatctgca tgactttgag
     3001 atcgtaaata tggcgtgact ctagcacata ttgctgcccg gtgtaggggt tctcgttctg
     3061 accgagatag tccgtcagca gttcaaacgg gcgcaccgcc gggtttacca ccactgcggg
     3121 cagcataaaa cattgtgaca accaggtggc gtaatatccc cccagtgacg aaccgacaat
     3181 acccagcgaa tcgccgccat gttccaggac aatggattcc agcagctctg ccgcgtcgga
     3241 aggatacggc ggcaactgcg gaatgatcat ctcaacgtca gggtgatgtt ccgccagcca
     3301 gttttttaac aagctcgctt ttgcagagcg cggcgagctg ttgaaaccgt gtaaataaag
     3361 aagcgtagac atcagtagcc ttctgaagcg gtatcaggtt ggaaacgtgt gtccgccagg
     3421 cgatgcacct cggtggtcag cgtgccatca gcatgtaact cgagagtacg ccagccgggc
     3481 gcgatggtgt ccagcgtaaa gttggaacag tgcggcttaa actgcacaca ggtcgacggc
     3541 gttgccagca ggcggcgacc gttccagtcg agatccagct cctgatgaat atgaccgcac
     3601 agcaagtatt tgacgtgcgg aaacttcgcc agcacggtat ccagttcgcc cgcgttacgc
     3661 aggctgtgtt gatcaagcca actacaaccc gcaggtagcg gatgatgatg cagcagcagc
     3721 aacgtatggc gttctggcgc atcggccagt ttacgttcca gccactcaag ctgaaactcg
     3781 ctcagctcac cgtgcggcac gccaaacacc tggctatcca gcaacaggat ttgccattgc
     3841 tcaccaataa acacgcgctt cgccggggag atacccgcat cctgtaacgc gctgtacatc
     3901 gcgggctgga aatcgtggtt gcccggcagc cagacgcagg gcgcacgaaa acttgcgatg
     3961 ccttcagcga aatgctgata ggccgcagag gattgatcct gcgctaaatc acctgtcgcg
     4021 acaatcaggt cgaattcgtg ctgatgtgga cgaatcgcct ccagcaccgc ctggtagctt
     4081 tcccaggtgt ttacccctaa cagggcttcg tgcttttgtg caaacaggtg agtatcggta
     4141 atttgtaaaa tcctgactct ggcctcacca gccagaggaa gggttaacag gctttccaaa
     4201 tggtgtcctt aggtttcacg acgctagtaa accggaatcg ccatcgctcc atgtgctaaa
     4261 cagtatcgca accagtccgc taaaaactgg ttaatttgat gcttttcgtc gcgttgatgc
     4321 aactttttat taggataatc ataccgcgct ttgaagcgaa aaatctgctg gcttgaacac
     4381 acttcagcca ccatcgcgtc atgatacaga cgcaccgtca ttgacggaag gctccagtaa
     4441 ctgatcgcgg gcgcagtctg ttctattgtc accagggtag tgtatcgggt cgattccaca
     4501 atcgtcagcc gatattgtgc gtttgccacc tgatagctta cagtttcgcc gggtgcgtca
     4561 ttgcgcggta acaaacggcg caattgtgaa aagttcatct cgcacaggcg catcatttca
     4621 ggaaagtcag gtgtgtaacg cttcatttat gcccactcat tttttaacgc ttgatgatgc
     4681 agctgcagcc attgcaaagc gatgaccgac gctgcgttgt cgattttccc ctcttctacc
     4741 cactggtatg cctgttcccg gcttaccaca tgaacgcgaa tatcttcgtt ttcatcagcc
     4801 agaccatgaa taccgcttgc ggtcgtggcg tccacttcgc ccaccataat tgacgaacgc
     4861 tcactggtgc cccccgggct tgccaggaaa cttaacaccg gtttggtccg tttgactatc
     4921 agtcccgcct cttcaatcgc ttcgcgacgg gcaacatctt ccacactttc accctcttct
     4981 atcatcccgg caaccatctc cagtagccag ggggtttcac tggtgtcgta tgcggcaatc
     5041 cgaatctgct caatcagcac aacttcatca cgcactgggt caaagggtag caagactgcg
     5101 gcgtgaccgc gctcaaaaat ttccctccgt acctcatgac tcatttgccc gttgaataaa
     5161 cgatgacgaa atctataaag atctaatgaa aaaaagccgc gataaagtgt ttctcgtgca
     5221 ataatttcta catcgttttt gccaaatgta acgggcaggt tgtctggctt aagcattgtt
     5281 aatgtcctgg cactaatagt gaattaaatg tgaatttcag cgacgtttga ctgccgtttg
     5341 agcagtcatg tgttaaattg aggcacatta acgccctatg gcacgtaacg ccaacctttt
     5401 gcggtagcgg cttctgctag aatccgcaat aattttacag tttgatcgcg ctaaatactg
     5461 cttcaccaca aggaatgcaa atgaagaaat tgctccccat tcttatcggc ctgagccttt
     5521 ctgggttcag ttcgttgagc caggccgaga acctgatgca agtttatcag caagcacgcc
     5581 ttagtaaccc ggaattgcgt aagtctgccg ccgatcgtga tgctgccttt gaaaaaatta
     5641 atgaagcgcg cagtccatta ctgccacagc taggtttagg tgcagattac acctatagca
     5701 acggctaccg cgacgcgaac ggcatcaact ctaacgcgac cagtgcgtcc ctgcagttaa
     5761 ctcaatccat ttttgatatg tcgaaatggc gtgcgttaac gctgcaggaa aaagcagcag
     5821 ggattcagga cgtcacatat cagaccgatc agcaaacctt gatcctcaac accgcgaccg
     5881 cttatttcaa cgtgttgaat gctattgacg ttctttccta tacacaggca caaaaagaag
     5941 cgatctaccg tcaattagat caaaccaccc aacgttttaa cgtgggcctg gtagcgataa
     6001 ccgacgtgca gaacgcccgc gcgcagtacg ataccgtgct ggcgaacgaa gtgaccgcac
     6061 gtaataacct tgataacgcg gtagagcagc tgcgccagat caccggtaac tactatccag
     6121 aactggcagc gctgaatgtc gaaaacttta aaaccgacaa accacagccg gttaacgcgc
     6181 tgctgaaaga agccgaaaaa cgcaacctgt cgctgttaca ggcacgcttg agccaggacc
     6241 tggcgcgcga gcaaattcgc caggcgcagg atggtcactt accgacgctg gatttaacgg
     6301 cttctaccgg gatttctgac acctcttata gcggttcgaa aactcgtggt gccgctggta
     6361 cccagtatga cgacagcaat atgggccaga acaaagtggg cctgagcttc tcgctgccga
     6421 tttatcaggg cggaatggtt aactcgcagg tgaaacaggc ccagtacaac tttgttggtg
     6481 ccagcgagca actggaaagc gcgcatcgta gcgtcgtgca aaccgtacgt tcctccttca
     6541 acaacattaa tgcatctatc agtagcatta acgcctacaa acaagccgta gtttccgctc
     6601 aaagctcatt agacgcgatg gaagcgggct actcggtcgg tacgcgtacc attgttgatg
     6661 tgttggatgc aaccaccacg ctgtacaacg ctaagcaaga gctggcaaat gcgcgttata
     6721 actacctgat taatcagctg aatattaagt cagccctggg tacgttgaac gagcaggatc
     6781 tgctggcact gaacaatgcg ctgagcaaac cggtttccac taatccggaa aacgttgccc
     6841 cgcaaacgcc ggaacagaat gctattgctg atggttatgc gcctgatagc ccggcacccg
     6901 tcgttcagca aacatccgca cgcactacca ccagtaacgg tcataaccct ttccgtaact
     6961 gatgacgacg acggggcttc ggccccgtct gaacgtaagg caacgtaaag atacgggtta
     7021 tctgccgcat tcttccccct tctcgcttca atttcgacca gccatcctct attctgatgg
     7081 gtatttacca ctggtcccgg aagacaaaaa tgaaacggac aaaatccata cgccacgcat
     7141 cgttccgcaa aaactggagc gcgcgccatc tgacaccagt cgctctcgcg gttgccactg
     7201 tttttatgct ggctggctgt gaaaagagtg atgaaacagt gtctctctat caaaatgctg
     7261 acgactgttc agctgcaaac ccaggcaaaa gcgccgaatg taccaccgcg tacaacaatg
     7321 cgctgaaaga agccgaacgt actgcgccga aatacgccac ccgtgaagac tgtgttgctg
     7381 aatttggtga aggtcagtgc cagcaggcac cagcccaggc tggcatggca ccagaaaacc
     7441 aggcgcaggc ccagcaatcc agcgggagtt tctggatgcc gctgatggcc ggttacatga
     7501 tggggcgtct gatgggcggc ggcgcgggat ttgcacagca gccgctgttc tcctcgaaaa
     7561 acccagccag cccggcttac ggtaaatata ccgacgcgac gggtaaaaac tatggcgcag
     7621 cccagccagg ccgcaccatg accgtaccga agacagccat ggcaccaaaa ccggcgacca
     7681 ccactaccgt tacccgtggc ggttttggtg aatctgttgc caaacaaagc actatgcagc
     7741 gtagcgcaac tggcacctct tctcgttcaa tgggtggctg ataccgatgg aaagagtcag
     7801 tattaccgag cgcccggact ggcgtgagaa agcccacgaa tacggtttca attttcacac
     7861 catgtacggc gagccgtact ggtgtgaaga tgcttactac aagttgaccc tcgcccaggt
     7921 tgaaaagctg gaagaagtca ccgccgaact gcaccagatg tgcctgaaag tggtggaaaa
     7981 agtgatcgcc agcgatgagc tgatgaccaa attccgcatt ccaaaacaca cctggagttt
     8041 tgtgcgtcag tcatggctga ctcaccagcc atcgctttat tcgcgtcttg atctggcatg
     8101 ggatggcact ggcgaaccta aactgctgga aaataacgcc gatacaccaa cgtcactata
     8161 cgaggcggcg ttctttcagt ggatctggct ggaagatcag cttaacgccg gtaacttgcc
     8221 ggagggcagc gaccagttta acagtctgca agaaaagcta atcgatcgct ttgtcgagtt
     8281 gcgtgaacag tatggcttcc agttgctgca tctcacctgc tgtcgcgaca cggtggaaga
     8341 tcgcggaacc attcagtatt tgcaggactg cgcgacggaa gctgaaatcg ctaccgagtt
     8401 cctctacatt gacgatatcg ggttaggtga aaaaggtcag ttcacggatt tacaggatca
     8461 ggtaatttcc aacctgttca agctgtatcc atgggaattt atgttgcgtg agatgttctc
     8521 caccaagctg gaggatgcag gcgtacgctg gctggaaccg gcgtggaaga gcattatctc
     8581 caacaaagcg cttctaccgc tactgtggga gatgttcccg aatcacccga acctgctgcc
     8641 cgcttatttt gcggaagatg atcatccgca aatggaaaaa tatgtggtta aaccgatctt
     8701 ctcccgtgaa ggcgcaaacg tgtcgatcat tgagaacggc aaaaccattg aagcagcgga
     8761 aggtccgtat ggcgaagaag ggatgattgt tcagcaattc cacccgttac cgaaattcgg
     8821 cgacagctat atgctgattg gtagctggct ggtgaacgat caacctgccg gaattggcat
     8881 tcgtgaagac cgtgcgttga tcacccagga tatgtctcgg ttttatccac atatttttat
     8941 tgagtaatca atgatgccgg atggcattcg ccatccggta attgttaacc tatctgcacc
     9001 gacagcatac tcaggctgcc catttctata ccatcgaccg gaatggtaat tggctcctgc
     9061 ccatcccacg cacctaagac atacaacaac ggcagatagt gctccggcgt tgggttcgat
     9121 aacgcgccac cttcatggtc gaggtaattc accagaggat gctgctctac cgccccttgc
     9181 cacgtcagat tcgctttcac atactcatta aacgacatcg cccaaggata tggtgaacta
     9241 tcaccgtgcc acttcactgt acgcaggtta tgcaccacgt tgccactggc gaccaacatt
     9301 attccttcat ctcgcagcgc tgccagtttg cgccccattt cgaaatgcca ggcggcaggt
     9361 ttgctactgt cgatactcaa ctgcaccatc gggatatcgg cgtcaggata catcttaatc
     9421 agcacgcccc aggaaccgtg gtcaaagccc caggcttctt tatccagcgt caccgggacc
     9481 ggcgctaaca gctcaaccag atgctgtgcc agcacaggcg aacccggagc aggataatgc
     9541 gtatcgtaca gcgcctgcgg gaagccacca aagtcatgaa tcgtgggcgg cgtctccatc
     9601 gcggtcactc ctgttccacg ggtaaaccag tgagccgaaa ccaccacaat cgcttgcggg
     9661 cgtggcaatg tcatccccaa cttctgccag ctgcgggtat acaaattatc ttccagcacg
     9721 ttcatcggac taccgtgacc taaaaacaat gctggcatac gtgttgaaga catgatgata
     9781 tccttaacta aaggtgtcat tttgatatcc tcacaatacg cttgttcggc ggagtaagaa
     9841 cccggataac aatgatgatg atcatcagtt attttgacga tctgcctgaa ggtgaagatt
     9901 tataaggagt tgtcgatgtc agtacctctc attctgacca tactggcggg ggcagccacg
     9961 tttattggcg cgtttctcgg cgttctcggg caaaaaccct cgaaccgctt actggcgttt
    10021 tcgctaggat ttgcggcggg gatcatgttg ctcatctcat taatggaaat gcttcctgcc
    10081 gcactagcag ctgaaggaat gtcgcctgtg ttgggttatg gaatgtttat cttcggtctg
    10141 cttggctatt ttggcctgga ccgcatgttg ccacatgcgc atccgcagga tttaatgcaa
    10201 aaatcggtgc agccgttgcc aaaatcgatc aagcgcacag ccattctgct cactctcggc
    10261 atcagtctgc ataacttccc ggaagggatt gccacctttg tcacggcgag cagcaacctg
    10321 gagctgggat ttggcatcgc actggccgtc gctttgcaca atatccctga aggtctggca
    10381 gtggcaggcc cggtttatgc ggcaacgggg tctaaacgca ccgcgattct gtgggcgggg
    10441 atttctggac tagcagaaat tcttggtggt gtgctggcgt ggttaatcct cggtagcatg
    10501 atttccccgg tggtcatggc ggcaatcatg gcggcggttg caggaattat ggtggcgctc
    10561 tcggttgatg aattaatgcc gctcgccaaa gagattgacc ctaataataa ccccagctat
    10621 ggcgtactat gtggaatgtc agtgatggga ttcagtttag tgctgctaca aacggcggga
    10681 attggttaaa aacaacatca gaagttgtcc agcacatgga caacttctgc cagcttcacg
    10741 aaagccttat tctacctttt cggctgtctc ttctctcgta ctgtttaagt atttgttccg
    10801 cgcttcaagc agtgcgtctc tgtcctggcg aaacgcccga caataacgtt tcaaatgcag
    10861 gaaataaccc accacaacca gagcaatcaa tacaatccag taccaggcga taaacatcct
    10921 tttaccctta atatttaaca atactaaaca tttagcgcat aaatttcaca tatccttttt
    10981 cggatatatt catatggtcg cataataacg gaacaattat atgattttga tcataatgaa
    11041 aaagaatgcc tgatattaat gaaattttct catagaaaga gaagcggaat taacatcttg
    11101 catagcgggg caaaataaaa accgaagcca gctacaactt cggtttcttg attaaggcag
    11161 taattaagca gcggttttca gctggcttta cgctcatgtg cctgacggta tgccaccagg
    11221 tcttcaatag tcacgagcgc catattgtgt ttattggcaa actcaatgca ctctggtgca
    11281 cgcgccatcg tgccatcgtc attagtcagc tcacacagca caccagccgg tttaaagcct
    11341 gccagcgtca tcaaatcaat agtcgcttca gtatgaccgc cacgcgtcag tacaccacct
    11401 gcctgcgcgc gaagtgggaa aacgtggccc gggcgattca gatcagaagg ttttgcgcca
    11461 tcggcaatcg ctgcgcgaac ggtcgtaata cgatcagcgg cagaaacacc ggtagtcaca
    11521 ccttcagccg cttcaatggt cacggtaaaa ccggtgccat aggcgctggt gttattttct
    11581 accatcattg gcagatcaag ttgtttacgg cgatcttcag tgatgcacag gcaaacaata
    11641 ccgctaccgt ggcgaatggt cagcgccatc tgctcaacgg tcatggtttc tgccgggaag
    11701 atcatatcgc cttcgttttc acggtcttca tcatcaagca ccattacacc gcgtccttca
    11761 cgcagcgcag ccagtgcatt ttcaacacgt tcgaaaggcg taccaaaaga ggaaagtagc
    11821 gtctgattca tggtaaaaaa acctcactaa aattatggtt accagaatca gggcagtctt
    11881 aggagtggcg gcatatagcc aaaataacgt gagcgggtcc atgcccgaca gaatcgttac
    11941 tctctcccat ccggactcta accgtcggcc ccggaattac accggatctg ctgtcctttg
    12001 agttcgcacc caaagcgctc gcgggctttc aactgagttg atttaccgcc ggtggggaat
    12061 ttcgccccgc cctgagaata agcggattca ctataacgct aatgattagc ggcagcaacg
    12121 catagcttca cataattctg gtttatgact tacccttatc gcactacaat ggcactcaac
    12181 acctatcagt acagggaaac tacaatgatt gacccgaaaa aaattgagca aatcgctcgc
    12241 caggttcacg aatcaatgcc taaaggaatc agggagttcg gggaagatgt ggagaaaaaa
    12301 atccgccaaa ccctacaagc tcagctgacg cgcctcgacc ttgtaagccg tgaagagttc
    12361 gacgtccaaa cgcaagtcct gttacggact cgtgagaaac tggcgctgct tgaacaaaga
    12421 atgagcgaac tggaaaatcg cagtactgag attaaaaagc agccagatcc agagactctc
    12481 cccccaacgc tgtaaacatt tgtctttaat gcactgtaaa ttatgaatta atcctctaat
    12541 ttacagtgct ttctattacc ttatcgctt
//
LOCUS       DAPGWD010000121        12467 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPGWD010000121 DAPGWD010000000
VERSION     DAPGWD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.122"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(48..329)
                     /locus_tag="RHX88_003309"
     CDS             complement(48..329)
                     /locus_tag="RHX88_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDV2231970.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNCYD"
     gene            complement(592..1701)
                     /gene="apbC"
                     /locus_tag="RHX88_003310"
     CDS             complement(592..1701)
                     /gene="apbC"
                     /locus_tag="RHX88_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDV2231971.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1833..3866
                     /gene="metG"
                     /locus_tag="RHX88_003311"
     CDS             1833..3866
                     /gene="metG"
                     /locus_tag="RHX88_003311"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDV2231972.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWAVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4007..7801
                     /locus_tag="RHX88_003312"
     CDS             4007..7801
                     /locus_tag="RHX88_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDV2231973.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKVNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALKERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWVRGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7811..11443
                     /locus_tag="RHX88_003313"
     CDS             7811..11443
                     /locus_tag="RHX88_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDV2231974.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPAQTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIATLPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVANDPTAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11504..11821
                     /locus_tag="RHX88_003314"
     CDS             11504..11821
                     /locus_tag="RHX88_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231975.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         3275 a         3164 c         3045 g         2983 t
ORIGIN      
        1 ataaatgatt tcggctttct aaatgcaatt aaaatcaggt tgccaaatta atcgtagcaa
       61 tttaacacga ttcgacgttt gctatcgcta cctgcaacaa ccacttctgt cgtaacacct
      121 ttgacgggcg aagaaacctg atgacgggtg tcaacattat acgttaactg accacaatgc
      181 tgttgtggtt gactattaac catagccata ctgcatgttg ggggactgat cctgccatta
      241 atcgttaatg tggcagtctc tgaagagaat gcgggagaag ccaatccata ggcaagaaaa
      301 ataattcctg acagccaata cttattcatt gaacgttatc cctgtagtaa aggttatgcc
      361 tggatagaat gagtgcataa caaactatag ctgtacatcc actacacagc cacgaaggat
      421 gataatgaag cattgcctgt atgatcaatc gactttgtag agtttcggac gaaggtccgc
      481 agaatattcg cagtattaaa taagtgttca ttaatgcggc aaaaactaat acaccgcatc
      541 aatgtaacat ctctgcggtg tatgaagaat aaccagaatg gttaataggc gttagaccgc
      601 gcggaaggaa atctcgcctg gaatgacttc accctgccag tagagctgag ctgcaacgcg
      661 gtcagccagc tggcggtaga tagcggtaaa ttcgctctcc ggacggctga taaccgttgg
      721 cgtgccttta tcgagatcct cacgtaagga gatgtgtaac ggcatctgcc ccagcagctg
      781 tgtgtggtat ttttcagcca gtttctctgc accaccggta ccgaaaatcg gttcatggtg
      841 accacagtta ctgcaaatat gcacactcat gttctcgaca ataccaagca ccggaacctc
      901 gactttctcg aacatcacaa tgcctttttt cgcatcgatc aacgcgatgt cctgcggggt
      961 ggtgaccacg actgcaccag ttactggaat gttttgcgcc agcgtcaatt gaatgtcacc
     1021 ggtgcctggc ggcatatcaa gcaccagata gtcgagatcc ggccacaagg tttcctgcaa
     1081 catctgcatt aacgccttgc tcgccatcgg tccacgccac accatcgcat tgtcgtcagt
     1141 gaccagataa ccaattgaat tggttgccag gccatgagac ataatcggtg ccatatgggt
     1201 accgtccggc gaagttggac gctgattttc tgcgcccagc atggtgggaa ttgatgggcc
     1261 gtagatatcg gcgtccagaa tgccgacttt cgccccttcc gccgccagtg ccagcgccag
     1321 gtttaccgcc gtggaggatt tacccacgcc gcctttaccg gaactgaccg caatgatgtt
     1381 cttcacacca ttaatacccg gctgattctt cacgcgtttt aaggtggcga tattgtgcga
     1441 cagtttccag tcgatagctt tcgccccggt gatacgcagc agttcggcgc tacattgctc
     1501 ttttaactct tcaaacgcgc tatgccagac gaatggcata accagttcca catgcaacgt
     1561 gtcgtccatc caggcgacat ggtgcaacgc tttcagcgtg gtgaggttat gcttcagggt
     1621 tgggtgctga aaattggcca gtgtcccggc gaccattgcg cgcaaggctt ctggtgattt
     1681 ggcctgggat tgttcgttca tcccgactcc ttttttgtat agataaacca tcagctgata
     1741 gtttacctga agaatataga gaagtactta cttaacattt tcccatttgg tactatctaa
     1801 ccccttttca ctattaagaa gtaatgccta ctatgactca agtcgcgaag aaaattctgg
     1861 tgacgtgcgc actgccgtac gctaacggct caatccacct cggccatatg ctggagcaca
     1921 tccaggctga tgtctgggtc cgttaccagc gaatgcgcgg ccacgaggtc aacttcatct
     1981 gcgccgacga tgcccacggt acaccgatca tgctgaaagc tcagcagctc ggtatcaccc
     2041 cggagcagat gattggcgaa atgagtcagg agcatcagac tgatttcgca ggctttaaca
     2101 tcagctatga caactatcac tcgacgcaca gcgaagagaa ccgccagttg tcagaactta
     2161 tctactctcg cctgaaagaa aacggtttta ttaaaaaccg caccatctct cagctgtacg
     2221 atccggaaaa aggcatgttc ctgccggacc gttttgtgaa aggcacctgc ccgaaatgta
     2281 aatccccgga tcaatacggc gataactgcg aagtctgcgg cgcgacctac agcccgactg
     2341 aattgatcga gccgaaatcg gtggtttctg gcgctacgcc ggtaatgcgt gattctgaac
     2401 acttcttctt tgatctgccc tctttcagcg aaatgttgca ggcatggacc cgcagcggtg
     2461 cgttgcagga gcaggtggca aataaaatgc aggagtggtt tgaatccggc ctgcaacagt
     2521 gggatatctc ccgcgacgcg ccatactttg gttttgaaat tccgaacgcg ccgggcaaat
     2581 atttctacgt ctggctggac gcaccgattg gctacatggg ttctttcaag aatctgtgcg
     2641 acaagcgcgg cgacagcgta agcttcgatg aatactggaa gaaagactcc accgccgagc
     2701 tgtaccactt catcggtaaa gatattgttt acttccacag cctgttctgg cctgccatgc
     2761 tggaaggcag caacttccgc aagccgacca acctgtttgt tcatggctat gtgacggtga
     2821 acggcgcaaa gatgtccaag tctcgcggca cctttattaa agccagcacc tggctgaatc
     2881 attttgacgc tgacagcctg cgttactact acactgcgaa actctcttcg cgcattgatg
     2941 atatcgatct caacctggag gatttcgttc agcgtgtgaa tgccgatatc gtgaacaaag
     3001 tggttaacct ggcctctcgt aatgcgggct ttatcaacaa gcgttttgac ggcgtgctgg
     3061 caagcgaatt ggctgacccg cagttgtaca aaaccttcac tgatgccgct gaagtgattg
     3121 gtgaagcgtg ggaaagccgc gaatttggta aagcagtgcg cgaaatcatg gcgctggctg
     3181 atctggctaa ccgctatgtc gacgagcagg ctccgtgggc ggtggcaaaa caggaaggcc
     3241 gcgatgccga cctgcaggca atttgctcaa tgggcataaa cctgttccgc gtgctgatga
     3301 cttacctgaa gccggtactg ccgaaactga ccgagcgcgc agaagcattc ctcaatacag
     3361 aactgacctg ggatggtatc cagcaaccgc tgctgggcca caaagtgaat ccgttcaaag
     3421 cgctgtataa ccgcatcgat atgaagcagg ttgaagcact ggtggaagcg tctaaagaag
     3481 aagtgaaagc agctgccgcg ccagttactg gcccactggc agatgatccg attcaggaaa
     3541 ccatcacctt tgacgacttc gcaaaagttg acctgcgcgt ggcgctgatc gaaaacgcag
     3601 agtttgttga aggttctgac aaactgctgc gcctgacact ggatctcggc ggtgaaaaac
     3661 gcaatgtctt ctccggtatt cgttctgcct acccggatcc gcaggcactg atcgggcgtc
     3721 acaccattat ggtggccaac ctggcaccgc gtaaaatgcg ctttggtatc tccgaaggca
     3781 tggtaatggc tgctggtcct ggcgggaaag atatcttcct gttaagcccg gatgctggtg
     3841 ctaaaccagg ccatcaggtg aaataatccc cctttaaggc gctgcatcga cagcgccttt
     3901 tctttataaa ttcctaaagt tgttttcttg cgattttgtc tctctctaac ccgcataaat
     3961 actggtagca tctgcattca actggataaa attacaggga tgcagaatga gacactttat
     4021 ctatcaggac gaaaaatcac ataaattctg ggcagttgag caacagggaa acgagttgca
     4081 tatcagttgg ggaaaagttg gcaccaaagg gcaaagccag ataaaaagtt tttcagatgc
     4141 tgcggcagcg gcaaaagcgg agcttaagct gattgcggag aaggtgaaga aggggtatgt
     4201 ggagcaagcg aaggttaatt ctttgcaacc ttcccaaacg gtaacgggct ctctcaaggt
     4261 agcggactta tccaccatta ttcaggaaca accctctttc gtagcagaaa cccgtgcgcc
     4321 tgacaaaaat acagatgctg ttttaccgtg gctggcgaaa gatattgctg tcgtttttcc
     4381 gcccgaagtt gtacacacca cgttaagtca tcgccgcttt cccggagttc ctgttcagca
     4441 agcagacaaa ttgacccaat tacgtcgctt agcctgtagt gtgtcgcaac gggataataa
     4501 aacagccaca tttgacttca gcgcctgttc tttagaatgg caaaacaccg tcgcccaggc
     4561 gatcagtcag atcgacggcc tgaaaacaac acagctacca tcaccagtaa tggctgtact
     4621 cacggcactt gaaatgaaat gcacaagata taaagtgcgt gaggatgtta tggatcagat
     4681 cgtccaggaa ggcggtctgg aatatgctac tgatgtaata atacaccttc aacagattga
     4741 tattaaatgg gattatgcga ataatgtcat tattattctg ccgtctggca ttgcacctga
     4801 ctacttggag caatattcca gatttgaatt acgcctacgt aaacatttat cactggcgga
     4861 agagtctctc tggcaaaaat gtgcacaaaa acttattgcc gcaattccac atattccaga
     4921 atggcggcaa ccattaattg ctttgttatt acccgaaaaa ccagaaattg cacatgaaat
     4981 tgcccagcgt ctactggggc aaaaaaaatt accctcgctt gagtggttaa aaatagtggc
     5041 gactgatgag cacattcttg cctcattaga aaaatatcac gagccatatg ccatttttga
     5101 tgattactat tgtggtgcga tatggtcagc caccgtatta caggagcaag gtgttgcagc
     5161 cctgccccga tttgctccct atgccgcaag tgactactgc gccgatgtgt tgcgtcatat
     5221 caatcatccg ttcgcattaa cactgcttat acgtgtagcc gggcatacta aacgctgtca
     5281 cgatcggatg acgaaagcct gcgctgcgtt cccacatgca gcaatggcgg cactgacgga
     5341 acttcttggg caaaaagaag agaacagttg gcgcattatg ctaatgacaa tgcttatctc
     5401 acaaccagca ctggcagaac aggtcattcc ctggctctcg acacccgcag ttgccgtact
     5461 gaaatcatgc cagcaacaac tgacacagcc ctcaaaccat gccagcgccg atctactgcc
     5521 agccgtagta gtctcccctc cctggctttc gaaaaagaaa aaatcgccga ttccggtgct
     5581 ggatttagcg ccattaggca ttgagccaat ctgttatctg acagaagaaa tcagtaatca
     5641 acttttggcg aaatatatct ggtattcaaa acacatcacg gttagccatg aagaaagtac
     5701 taccaacctg ttggcaagga tgggttttca acgacggatc gctggtacat atattaaagc
     5761 tcccgaagcg gtagttgagg catggctaaa tgaagattat tcaaccttac taagtgaatt
     5821 taaggtgttt cattcaccta ccgggcatta ttggcagttg gggattttga caacattgcc
     5881 gctggagaaa gcagtaaaag catggaatgc ccttacccta tctccacata ccgataccga
     5941 atacgccatg ttacattttg gactcaaagg gttacctggg ttagtaaact cacttgcacg
     6001 ctatccacaa gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc
     6061 cgtcgcccgt gccttcaaca aactgaaaac gctacgcgaa aacgcccgta gctggctgtt
     6121 gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga
     6181 agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
     6241 actgcaagaa atcgcccgac gttataacca gccggaagta accgatgcgg tgaacgctct
     6301 gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cggcctttta
     6361 tcagccatcg ctctggacgc gcccggtatt aaaagcaaat gcccaatcac tgccagatag
     6421 cgccctcctc cacctcggtg aaatgctccg cttccctcag gaagaggctc tgtatccggg
     6481 attattgcag gtgaaagacg tctgttccgc cgactcactg gcgggatttg cctgggatct
     6541 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
     6601 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc
     6661 tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgctg ctatcggtag
     6721 tgatatcgcc cttatgcagc ttaacggcat cgcccagaaa ctgaaattca aagcattaaa
     6781 ggagcgggca aaagaaaaaa ttgccgacat tgccgagagc cgcgaactca cggtggcgga
     6841 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgc tgctgctgga
     6901 ttttggccca cggcagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
     6961 tgtttccggc agccgcctga aagacctgcc caaaccgaac aaaagcgatg atgaaacgcg
     7021 ggcgaacgat gcggttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccatcgccgc
     7081 ccagcaggta gcaaggctgg aatccgccat gtgcctgcgc cgccgctggt cgctggaaaa
     7141 cttccagctc ttcctggttg agcatccgct ggttcgtcac ttaacccgcc gtctgatttg
     7201 gggcgtttat agcgccgaaa accagctact ggcttgcttt cgcgtagcag aagataacag
     7261 ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcac
     7321 tcctcacgtt ctggaaatat caccaacgga tgctgccgcc tttggtcagc tttttgccga
     7381 ctacgaactg ctaccaccgt tccgccagct cgaccgtaac agctacgccc tgacagaagc
     7441 cgagcgcaat gccagtgaac tgacccgctg ggcaggcaga aaatgcccga gcggtcgggt
     7501 catgggactg gcgaataaag gttgggtgcg tggtgaaccg caggatggtg gctggatcgg
     7561 ctggatgatc aaacctctgg gccgctggtc gttaatcatg gaaatcgatg aaggctttgc
     7621 agttggtatg tcgccagccg aacttagcgc cgagcaactc ttaagcaagc tgtggctatg
     7681 ggaaggcaaa gcagaaagct atggctgggg gagtaattca acacaggaag cgcagttctc
     7741 cgtactcgat gccatcaccg ccagcgagct gattaacgat attgaagccc tgtttgaata
     7801 aggaaagagc atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa
     7861 atataaaaaa ggcaaaacac cactcagcca tcgcaattgg ccgggcgaac cagtgcctgt
     7921 tatcactgaa agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa
     7981 aaaaaacatt gtctggcgtt atgagaattt ttcactggag tggcagtccg ccattacgca
     8041 ggccatcaac ttgatcggcg aacacaaacc ctcaatcccg gcccagacaa tggcggcgct
     8101 agtctgtatc gcgcaaaatg acagccaaca gttgctcgac gaaatcgtcc aacaagaggg
     8161 gctggaatat gcgactgagg tggtgattgc acgccagttt attacacgat gttatgagag
     8221 tgatcctctg gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc
     8281 agaaacctat aacgaattcg atctccgact gcgtaagcat ctctctctgg cagaggaaag
     8341 ctgctggcag cgttgcgccg acaaactcat tgccacactg ccaggaataa ccaaagttcg
     8401 ccgccctttt attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag
     8461 ccttgaatgc ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctaatga
     8521 ccccacagcg gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga
     8581 agccagctac atgtcgcatg aaaaccactt cggctacgcg gcctgcgccg cccttttgcg
     8641 cgaacaagga ctggcagccg ttccgcgcct cgcgatatat gcccataaag aagattgcgg
     8701 cagtctgctg gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga
     8761 taaaaacaaa cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac
     8821 actcgccgca ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc
     8881 aatcatcgaa gacaaaaagc tgcctgcaca gcaaaaagca cgcgatgaat actggcgtac
     8941 gctgttacaa acgctgatag catcgcagcc acaactagca gaaagggtga tgccgtggtt
     9001 aagtactcaa gccagggcag tgctgaatag ttatttatcg gcaccgccaa aaccggttat
     9061 tgatagcacc gataacagca gtctgcctga aatgctcgtt tcaccaccgt ggcgtagtaa
     9121 gaaaaaaatg acagctccac gtcttgattt ggcaccgctc gaattaactc cgcaaattta
     9181 ctggcaacca ggcgaacaag agaggcttgc cgccactgag tctgcccgtt atttcagcac
     9241 ggaatctctt gcggaacgca tggaacaaaa aagtggacga gttgtattac aggaactggg
     9301 ttttggggat gatgtatggc tgtttctgaa ttatatactc cccggaaaac tggatgctgc
     9361 acgcaattca ctcattgttc agtggcatta ctaccagggg cgggttgaag agatcctgaa
     9421 tggctggaac tccccgcaag cacaattagc agaacaggcg ctccgcagcg gtcacataga
     9481 agcgttaatt aacatatggg aaaatgacaa cttctcacgt tatcgtccgg aaaagagtgt
     9541 ctggaacctg tatttattgg cacagttgcc gcgtgagatg gctttgacct tctggctgcg
     9601 tatcattgag aaaaagcatc tgttcgcggg tgaggactat tttctcagta tcctcggatt
     9661 ggatgcgcta ccaggtctgc tgttggcttt ttcacatcgt ccaaaagaaa catttccgtt
     9721 aattttaaat tttggcgcaa cagaactggc gctgcctgtt gcccgcgtct ggcgacgttt
     9781 cgcggcgcag cgtgatctgg ctcgccagtg gattttacaa tggccggaac atacggctag
     9841 tgcacttatc cctcttgtct ttaccaaacc cagcgataat agcgaagccg cattacttgc
     9901 cctgcgttta ctgtacgaac agggacatgg cgaattgcta caaaccgtgg caaaccgctg
     9961 gcagcgtaca gatgtatggt ctgccctgga gcagttgctt aaacagggtc caatggacat
    10021 ttacccggca cgcattccaa aagcccctga tttctggcat ccggcaatgt ggtccaggcc
    10081 gcgccttatc actaataatc aacctgttac cggtgacgct ctggaaatta tcggcgaaat
    10141 gctgcgcttt acccaggggg gacgttttta tagcgggctg gaacaactga aaacgttctg
    10201 ccagccacaa acgctggcag cttttgcctg ggatctcttc actgcgtggc aacaagctgg
    10261 tgcccccgca aaagacaact gggcatttct ggcgttaagt ctctttggtg acgaaagcac
    10321 ggcacgggat ctgacgacac agatcctcgc ctggccacaa gaaggcaaat ctgcccgtgc
    10381 tgtcagcggc ctgaacatcc ttaccctgat gaataatgat atggcgctga tacagctgca
    10441 tcatatatcg caacgggcta aatcccgccc cttacgtgat aacgcggcgg aatttcttca
    10501 ggtggtcgca gaaaatcgcg ggctaagcca ggaagagcta gcggacagat tagtcccaac
    10561 cctgggcctt gatgatccgc aggcgttgag ttttgatttt ggtccccggc agtttaccgt
    10621 tcgcttcgat gaaaacctca acccggttat ctttgatcag caaaacgttc gccagaaaag
    10681 cgttccccgg ttgcgcgccg atgacgatca actgaaagcg cccgaggcac tggcccgact
    10741 aaaagggcta aaaaaagatg ctactcaggt gagcaaaaac ctgctcccgc gtcttgaagc
    10801 tgccctacgt accacccgac gctggtcgct ggcagatttt catactctgt ttgttaatca
    10861 tccctttacc cgcctggtta cccagcgatt aatatggggc gtgtatccgg caaatgaacc
    10921 gcgtcgttta ctcaacgcct ttcgtgtggc cgcagagggg gagttctgca atgcgcagga
    10981 tgagccaatt gacctgcctg cggatgctct gattggcatt gcccacccgt tagaaatgac
    11041 agcagaaatg cgcagtgaat ttgcccagct ttttgccgat tacgaaatta taccgccact
    11101 tcgccagttg acacggcgca cggtgctgct cacgcctgac gagtcagcca gtaatagttt
    11161 gaatcgctgg gaaggtaaat ctgctaccgt tgggcaactt atgggaatgc gatacaaagg
    11221 ctgggagtca ggctatgagg acgcatttgt ctatgacctg ggtgagtacc ggctggtcct
    11281 taagttttca cccggtttta accactacaa tgttgatagc aaagcgctaa tgagcttccg
    11341 ttctcttcga gtgtacagtg acaataaatc cgtcactttt gccgaacttg atgtgtttga
    11401 tttgagtgag gcgttaagcg cacctgacgt cattttccat taaaacacag cgggcagtga
    11461 tgcaactgcc cgttatcaac acgacttacc agcggggaaa gcgatgatcg tgcaaaaaga
    11521 gctggttgct atttacgatt atgaggtccc tgtacctgaa gatccgtttt ccttcagact
    11581 tgagatccat aaatgctctg aattatttac aggttccgtc tatcgactgg agcgattccg
    11641 gctacgtcca acatttcatc aacgtgatcg agaagatgct gacccgctaa taaatgatgc
    11701 gttgatttat ataagagatg agtgtattga tgagcggaaa ttacgaggtg aatcacctga
    11761 aactgtaata gcaattttta atcgtgaact acagaatata ttcaaccaag aaatagaata
    11821 atatactcta gataattaaa gtcgcagcac atgcaacttg aagtatgacg agtatagctt
    11881 tcttaaaggg ggaaacggta ttccccctta gatgcgtttc aatttgcccg ttacttcaac
    11941 aaatcaatca accgtaacag agccactaat aactccagta cggcaatgag cacggttaac
    12001 ttttgtgtca gcgtgtccat ccgctctcct tattaaggag cgcaacacca gcaccttacc
    12061 cttacactgc ctgttgatac tggtttacgt atgttggtac gaaacgttgc tctccggatc
    12121 agggcaccgc ctgcggcacc acccgtccgg cagccaggac ttaaaattgg tccgatccgg
    12181 cacaacctcc caacgggcag gattataaat ctcactcact gaattgcaat ggagtgtgga
    12241 tatggttata atttattcgt tggtacgaaa tgttgcacaa tcagcaagtt agcgtcactg
    12301 tagtaaataa aagaacctgc ctcaccagca ggttttttta ttttctgcga tctactttcc
    12361 agatattttt tgccaatagc ccaacataaa tattcacatc ccggtcaaat ggtgcgttga
    12421 atataaaatc gatgaattta taaataaaaa tgagttatat gatgaat
//
LOCUS       DAPGWD010000122        12305 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPGWD010000122 DAPGWD010000000
VERSION     DAPGWD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.123"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..347)
                     /gene="quuD"
                     /locus_tag="RHX88_003315"
     CDS             complement(<1..347)
                     /gene="quuD"
                     /locus_tag="RHX88_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HDV2231976.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIR"
     gene            complement(365..1354)
                     /locus_tag="RHX88_003316"
     CDS             complement(365..1354)
                     /locus_tag="RHX88_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV2231977.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPEHYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCAHWVLSVVRRDLDFNDNHAV
                     TMPELCWWLVRNDLADALPESAARKALRLPKSVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEQYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(1362..2159)
                     /locus_tag="RHX88_003317"
     CDS             complement(1362..2159)
                     /locus_tag="RHX88_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDV2231978.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(2179..2568)
                     /locus_tag="RHX88_003318"
     CDS             complement(2179..2568)
                     /locus_tag="RHX88_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDV2231979.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACTAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(2565..2891)
                     /locus_tag="RHX88_003319"
     CDS             complement(2565..2891)
                     /locus_tag="RHX88_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDV2231980.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEVAGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(2891..3385)
                     /locus_tag="RHX88_003320"
     CDS             complement(2891..3385)
                     /locus_tag="RHX88_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDV2231981.1"
                     /translation="MSMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQDPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQQERNNFLARRERCLRKSSKRAVSGEEWYLSGN
                     YVGA"
     gene            complement(3382..4323)
                     /locus_tag="RHX88_003321"
     CDS             complement(3382..4323)
                     /locus_tag="RHX88_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2231982.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDPSKSVPSKFDAPKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTDEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(4313..4492)
                     /locus_tag="RHX88_003322"
     CDS             complement(4313..4492)
                     /locus_tag="RHX88_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDV2231983.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(4668..5225)
                     /gene="ymfL"
                     /locus_tag="RHX88_003323"
     CDS             complement(4668..5225)
                     /gene="ymfL"
                     /locus_tag="RHX88_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HDV2231984.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(5269..5469)
                     /locus_tag="RHX88_003324"
     CDS             complement(5269..5469)
                     /locus_tag="RHX88_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2231985.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            5560..6234
                     /locus_tag="RHX88_003325"
     CDS             5560..6234
                     /locus_tag="RHX88_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HDV2231986.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            complement(6449..6964)
                     /locus_tag="RHX88_003326"
     CDS             complement(6449..6964)
                     /locus_tag="RHX88_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231987.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            7109..7419
                     /locus_tag="RHX88_003327"
                     /pseudo
     CDS             7109..7419
                     /locus_tag="RHX88_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7434..7796
                     /locus_tag="RHX88_003328"
     CDS             7434..7796
                     /locus_tag="RHX88_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231988.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYIRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGR"
     gene            7862..8686
                     /locus_tag="RHX88_003329"
     CDS             7862..8686
                     /locus_tag="RHX88_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HDV2231989.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            8814..9350
                     /gene="yfdR"
                     /locus_tag="RHX88_003330"
     CDS             8814..9350
                     /gene="yfdR"
                     /locus_tag="RHX88_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV2231990.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            9341..9703
                     /locus_tag="RHX88_003331"
     CDS             9341..9703
                     /locus_tag="RHX88_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231991.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            9703..10008
                     /locus_tag="RHX88_003332"
     CDS             9703..10008
                     /locus_tag="RHX88_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2231992.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPVE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMTQQEVK"
     gene            10235..11398
                     /locus_tag="RHX88_003333"
     CDS             10235..11398
                     /locus_tag="RHX88_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2231993.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(11414..11490)
                     /locus_tag="RHX88_003334"
     tRNA            complement(11414..11490)
                     /locus_tag="RHX88_003334"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11454..11456),aa:Arg,seq:tct)
     gene            11733..>12305
                     /gene="fimZ"
                     /locus_tag="RHX88_003335"
     CDS             11733..>12305
                     /gene="fimZ"
                     /locus_tag="RHX88_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDV2231994.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGK"
BASE COUNT         3266 a         3075 c         2889 g         3075 t
ORIGIN      
        1 cggatatcta atgccattaa catcccttca attatgccct cagccttctg taaccttttc
       61 ccgatataac catcagagca gcaatgctta cctgccagtg acatgaatgt cataccgact
      121 acataataat ctactaataa atcgtgcaaa tcgctgttgt tctttttcag acgggccatg
      181 cacccgcaaa tgatcatcgc gtcatcgtca caacattgcg ggcgagattt tacttttgaa
      241 ggaattaatc ccttaaaacc ggcggcaatg gacgaccagg tcacatcttc atgattatta
      301 gccgcccacg ctccccaacg ttcaagaacc atctgaatat cacgcattaa ctttctccac
      361 aaaatcaggc cagcacacca atcgccagtg cgcgatcgat aaaacgaaat atcagctcca
      421 gctgggagcc atacttctct tcaaatgcca cggtatccgc atgcagttcg tcgtggtgtt
      481 ttctgcacaa aggcagcaca aaaaggtcat gcgcttttgt acccattcca ccctgaccat
      541 ggcctatcag atgatgcgga tcatcagctg gctttccaca acatgcacac ggctgcgtct
      601 taacccagcg cgtgtactgt tcattaaccc agcgacgacg tttggggcgt aacataaaag
      661 actccggcga ctccggatcc actttcagcg ccagcacctt tttcgcttta tcctggataa
      721 tgctggtggc aggaaccgaa ggcacaaggt cactttcccg ggtgacagac ggcaaaacag
      781 acttcggtaa tctcagtgcc ttacgggctg cgctttccgg taaggcatcc gccaggtcat
      841 tacgaaccag ccaccagcac agttccggca ttgtcacggc atggttatca ttaaaatcga
      901 gatcccgacg gactacagac aacacccaat gggcacagtt atccgttgcc attgattcca
      961 gccgctccgt gaactgatca cgtaactgat tatcgcagtg ccagcacaga cggattgcgc
     1021 ccggcgcgtg tcgcattgtg gtcatgttct cgctgtgcca gtcggaatga ggccactggc
     1081 aacctttttc acgaagtaac cagctttcaa gacattccac cccaccagca cgacgaatca
     1141 ctgcctcatt acggaacacg gcccgaacgg caggatcatc cgccagcggt tgtgatgccg
     1201 cgggaacggc accactggcg aaagatgaat aatgctccgg ctcaggctcc agcagtacac
     1261 gcccctgcat aaacaggggc atcagctctg aaccgggcct gaacaatacg atccccatac
     1321 gcggggcaat ttcaggggtc agtagtgctc tcacggtcac ctcaatgaac ggtatcgagc
     1381 agctttaaca gctcagggaa tcgggattcg aagaaatgcg gctgcgtctc gcgcggattt
     1441 gcgggactgg tgatgttctt gccgaacatg cagcctttcg ctgtcagcga ccagaatttt
     1501 ttgatgttgt taatcgcggt acggctgtat cgttcgcgct gctcgacgat ccccagcttc
     1561 accatctggt gatatgcctg attagctgtc aggcggatac catactgctt cagcagtgca
     1621 ctcagtgaca gcgtgggacg gcttgagcca tcaggcgcgt cagcaggagc atcaatggca
     1681 tagcgcggtg ccagattcgg taagccaaca gcctcctgga gtttctgaca ggcaccaagc
     1741 actgaagagt tagacaggtt taactcccgg cgcataaagt ccagcagaat cacgccagcc
     1801 tgcatcttgt cagcagcctg cccggataat ttttccgatg cgctggttac catatcgaaa
     1861 gtacggatca ccttcagatg gaatgacggg ctgatccaca ttgcataggc atacaccagt
     1921 tctttgcaga catacgtccc ctggttattt ccgccacgaa taacgttaac tggctctata
     1981 ttgaccgagt tgcaaatctg caactcgctt attaaacgtt cagtttgctc attgcggagc
     2041 cagaatgcag gcttatgctt atccagagaa ccggcagccc tgtgcagatc gttcaggctg
     2101 taacgcccat aagcatcacg acgaacttca ataccatcaa tgaccatcag attattcata
     2161 cttcgtttct cctcttgatc aggcggctgc acccgccgtt ttctcgtact tactgatagt
     2221 gatctcgacc ttcccttccg ggataaccgg tccccactcc accagcattc ttttcacctg
     2281 gctgtcgtct tcccacacac ccgcgtgggt cagggcgtca aacagcgcct tgttatagtt
     2341 gtccagatcg cggatccggt tatccggagg aaacaacacg atctccactg aagcaggtgc
     2401 cgacgttggt ttcggcagac gacgtaactg ctcaactatt gctgtgcacg ccgcactctg
     2461 gaattttcgc cccgccgcgc ttatcaggct cttaccagca aatgcccctt tgttggggtg
     2521 tcgccagtag gtgttcacgc tgggtggaaa aggcagaatc agcttcatac tttcagcccc
     2581 ctctcatgta accagtgggc tgcacgcagc ctggcgtttt cctcaccagc aagcagtgcg
     2641 cggataatcc cggccacctc gctgtcgtcg tccttcaccg cggtatgaag cgtgatgccc
     2701 cgggccacgc cacgctttat cgtgatgacg ccttttttct ccagtgcgcg aagatgctcc
     2761 accgctgcat tcactgaacg gtatcccagc atggtagcca cctcctgatt ggttggcggg
     2821 aagccacgtt ctttctggta agaaatcagc atatccagca cctgctgctg gcattgagtt
     2881 aacgtcgtca ttaagccccc acgtaattcc ctgacagata ccactcttca cccgatacag
     2941 cgcgcttgct gcttttccgt aaacaccgct cacgacgcgc aagaaaattg tttcgctctt
     3001 gctgggagtg gctttcacgg aatgccgcca tccacaccgt tgcagcacga cggtataagc
     3061 ccctggactc cagttcttcc gcctggcggg tcaggcacaa aatcacccgc gggtcgttag
     3121 tgccgacata gaaattgcgc acaggtctgg tttcacgaac tggttgtggt tccggatcct
     3181 gcgctctctc agtcaggcgc gggaaatgtc tgcgtgtatc tccttcacaa cggtgagcca
     3241 cacgcccact ctgacgtaac ttgcttgctg actgcagaac gcgctgccgt gagtaacctg
     3301 caaaagcatc cgcaatgtct ccggaagtac agcccggatg ggcttcaatg aatttctgaa
     3361 cgtcattcaa aagactcatg ctcaccccct gaatcctgcc gggatctggc tgtagtccac
     3421 attgtcgtaa ctggctttga agtacgggtc ttcgcgtttt tctgtgtacg tgctgacgga
     3481 cggcgataag cgcagggaaa gctcatccca tttttcccgc agcttcgacg ggctgagcac
     3541 gttacggcac cagaacggat cgcggctgac gcggctgtac atctcgcaga tttgtttgtg
     3601 agtacgacca tcctgcacac acatcaggcg aatttcgttt gcccaggctg tccagttcgg
     3661 ttctttggga cgaaccacct cgccgtcaca ttcggcagcc tgctcgtaca gggcgatgat
     3721 ttttttccag agccactgtg cgcaggtcaa atcatcctgc gtcccccact ggcgcttttt
     3781 agggctgaat acaaccgcat caggatggcg agttaaaaaa tcctgttcat ccgtctgcgt
     3841 gtccggttgc gaagcgtccg gacgagaagt ttttttatct gacggatcat gttttgattt
     3901 tactgacgga tccccgccag attctgacgg gtgaaaaccc gcttttttac cagatttcgg
     3961 cgcatcaaat tttgacggga cagattttga cgggtcagat tttgacgggt cagaatctga
     4021 cagttgagaa aatgccgctg cctgaagctt cgcaacgtta agctgataaa cattcgacgc
     4081 attgcggtta ccctggcgac gcgccttacg cgttaaccag ccttctgctt ccagccgtgc
     4141 gatagccgtt ctgacggtac tcatccccgc gccaatctgg cgtgcaatgg tttcaattga
     4201 tggccagcac acaccttcgt cattactgaa atcagccagg cgggccataa ttgccacgct
     4261 ggataacttc atgcctgacg cagcgcaacc atcccataca tagccggtta atttagtgct
     4321 catgaccgac ctctatttcc ctgaatttac gacgaaactg ttcgagcggg ctgaagcact
     4381 catgctcata gccttcgcgg aggtagataa cccgttgtgt ttccggctcc caacgaatga
     4441 ctctgacggg tactccgtag tgatctttga accagcggtt aacttgtcgc aaaggactgt
     4501 ctccttctgc cggttgaaat cacccacagc ccactctgca aagctgtggg ttacaatttc
     4561 cctgtcacct ggtacattca ctgcatagca atattccacc ttcgcttttc cacccggtac
     4621 aggaagcgca atcagttgcg agcgacggta gtgtgttgtt aaactgttca tgcgttagtt
     4681 tctccacaac cagaagcaat cgacgccacg acgcccggag ctgcacactc gcgggcgtca
     4741 ttactttctg aaatgcaaaa aattttgtag acaagtgctg catgctcctg cagcttcgaa
     4801 attgagagat acagctcgtc gttaattgct gtcttctcat gcggttccac tacaccgtct
     4861 tcgattgctg aacgaatctg ttttgaataa ctgccgatct gttcaatgac ttccagcaga
     4921 cgctggttaa tatcggcgtt gtccacatcc tcgacgtcag gaagagacac aaagacgcca
     4981 tttgcagact gcgccacagc atcagcaatg aagtgagtgc caccagcacg ctgtaaaacc
     5041 attgcccatc ccagcgggaa aatctgatcg ccatctgcac gaaggcggtt gaataaagcg
     5101 ttttctgtta catcgagcca gtcagccgct tcagcgtaac cacccggcaa cgccgcgata
     5161 gtttttctga cagctttcac gtaccactca ggctgttttt ctattttcca gtgatgctta
     5221 cccacgatta gcctcatcgt tctgtggtta aaaattgaaa gtgttctgct aatctttcgg
     5281 atagatatcc ggtcttaagt cagatttcgt aattgcacct gacgtgcatt gctcaagttt
     5341 tttagccagc acaaaactgg cttttttata gccattgaaa accagccgta agtagccagg
     5401 tgttgagcca acttttccgg ccaactcgcc ctgctgttct ttggttaaag agtcccaata
     5461 cgctttcata caatatgtac ctccggtgta catattacat gattaaaatg aaccttcaag
     5521 atacttgtac cttaacggta caagggtttt aatttcgtta tgaaaacaat ccatgacatc
     5581 cggcggtcta acgccagaaa actgagagat ggtgttggcg ggaattcttc ctttgccact
     5641 atgattgatc gcgagccaac ccagaccagc aggtttatgg gagatggtgc tactaaaaat
     5701 atcggtgata gcatggcacg acacatcgaa aaatgtttcg acctgcctgt cggatggctc
     5761 gatcaagaac accagacaac gaacatcaca aaaaaacctg atgtttcaat cactaataaa
     5821 caaatcacat tagtccctgt catatcatgg gtacaggccg gagcatggaa agaagttgga
     5881 tattctgagg ttgatttgag cacagcagaa acgtatccct gccctgtacc ctgtggggaa
     5941 atgacttata tcttgcgggt gataggtgat tcaatgattg atgagtaccg cccgggagac
     6001 atgatttttg tcgatcctga agtacctgcc tgccacggtg acgacgttat tgcattgatg
     6061 cacgatacag gtgaaaccac cttcaaaagg ttgatagaag atgggacaca gcgttatctc
     6121 aaagcgttaa acccaaactg gcctgagcct tacattaaga tcaacggtaa ttgctctata
     6181 attggaactg tgattttctc aggaaaacca agaagataca aaatcaaagc ctaatcaatg
     6241 tttatgaacc tgcttcggca ggttttttta tacttgacaa tgtacctttg ggatacataa
     6301 tgtacccaag agaaacaaca aacaggcagg acgcccacga agtatccgcc tggggcatat
     6361 gaagttcagg atgattcgtt agcaacaaaa aagctcccta caggacgctt agctctttaa
     6421 caatctggat atccacaaca gtagtaatct acagattgcc gttaagtttt ctggacaact
     6481 cctcaatgga tggaggcgat acgtaatccg gatttttatt catcagaaac ttattttcac
     6541 agtggaggca cctgctttta tgaaaaagct catcttcgct aaccgggaat ggttgaagta
     6601 tcgatactat cttttgtcca aaacattttg ggcaaagatg catggttatg ctgccaccgt
     6661 tcacgattac ctccttcgag tatacaaaag tacccgactc aagttggtta aggatatagc
     6721 cttccgtctg agcctcaaag ttttcgaatt ctgcaatttt agctttgaga gaagcattta
     6781 tttcttgata agagcccacc agttcaacga gagacacgca ttcgcgctga atagacgcaa
     6841 gctttgagtt cagctcacca atagccgcat ttacttcagc ttgagttttt gcctcgttca
     6901 ttagttttgc aatctgggct gtttcacgaa tagccgtcat tgctgccgtt aattcagcga
     6961 tcacattgaa tactcttatt gttgttgggg atatccagat taaccgaatc cttgttgttg
     7021 gggaataacc aggtccacct cgcctgatgt ggctaaaagc aggcacataa cagctaagta
     7081 ttttcaacca gagagaatcc ttagcgttgt ggtgaatgcg gctcagcgca cgcgggttaa
     7141 ggttgaggct gacagtcgac cttctgtgga tacccacccg cctggtgtgc aaccttcgcc
     7201 aggcaccggg aggcacccgg caccacaact ttatgctgtg tgtagtcctg gcggtaccag
     7261 cttgtaccct tgcttccggc tggtaccgtc ctttttacaa aacagagaag agcatcaccg
     7321 gacgacgggc tcataaccca atccatccgg gcggcggcca ccacaggtgt tcttctctgt
     7381 tttgtggaga aactaatcgg ccttgcaggg tcgatatgat gaggagcagc aaaatggcta
     7441 gcgaacgcag tactgatgtg caggcattta tcggggagct ggacggcggc gtatttgaaa
     7501 ccaaaatcgg cgcagttctc agtgaagtcg cttccggtgt gatgaacacg aaaaccaaag
     7561 gtaaggtctc actcaacctg gaaatcgaac catttgatga gaaccgtgtg aaaatcaaac
     7621 acaaactctc atatattcgc ccgactaacc gcgggaaaat ttccgaagaa gacaccaccg
     7681 aaacgccgat gtatgtcaat cgcggtggtc gcctgactat tctgcaggaa gaccagggac
     7741 aattactgac tcttgccggt gaacctgacg gaaaactccg cgcagcaggt cgttaatatc
     7801 gttcgtaata aactgattat ttatctcatc actgaatatc tttatatagt gaggacttat
     7861 tatgtctcag aacttagacg caaccgcaat taatcaaatc catgccctta tttctgctca
     7921 gggtgttaat gaaattatca gtaagattgg tgccgatgct gtggcattgc ctgagaattt
     7981 ccgcattcat gatctggaaa aatttaattt aaatcgcttc cgtttccgtg gtgcactttc
     8041 cactgccagc atcgatgatt ttacccgtta ttctaaagat cttgcagatg aaggcacccg
     8101 ctgctttatc gatgccgata atatgcgtgc cgtcagtgtg cttaacctgg gtactattga
     8161 tgagccaggt cacgcagata acactgccac tctcaaactg aaaaagacag caccgttctc
     8221 tgctctgttg tctgttaacg gcgagcgtaa ctcccagaag tcactggcag aatggatcga
     8281 agactgggcc gactaccttg tgggctttga tgctaatggt gacgccattc aagcaacaaa
     8341 agcggctgcg gcagtccgta aaatcacgat tgaagcaaac cagaccgctg attttgaaga
     8401 taatgacttc agcggcaaac gctccctgat ggagtctgtc gaagcgaaga ccaaagacat
     8461 tatgccagtg gcatttgaat ttaaatgcgt tccgtttgaa ggtctgaaag aacgtccgtt
     8521 taaattacgc ctcagcatta tcactggcga tcgtcctgta ctggttctgc gcattattca
     8581 gctggaagcg gtgcaggaag aaatggctaa cgaatttcgt gatctgcttg ttgagaaatt
     8641 caaagacagc aaagtagaaa cctttattgg tactttcacc gcctgatttc attactgcaa
     8701 atgcccctgc gggggcattt atggaaacgt aattaactca ataatcaccg gatggtgagg
     8761 gcttcctttt accagaattc agcgcggtgc agtgcatata cgtggagaac aaaatgtcat
     8821 ttattaaaac tttttccggg aagcattttt attatgacaa gataaataaa gacgacatcg
     8881 atattaacga tatcgcggtt tccctttcaa atatctgtcg ctttgccggt catctttcgc
     8941 acttctacag cgtcgcccaa catgcggttc tttgcagcca gctggtgccg caggaatttg
     9001 cttttgaagc gttaatgcat gatgcaacag aagcgtattg ccaggacatt cccgcaccac
     9061 tgaaacgcct tcttcctgac tataaacgga tggaagaaaa aatagacgcc gtaatccgtg
     9121 agaaatacgg gttaccccca gttatgagta cacccgtgaa atatgccgat ctcatcatgc
     9181 tggcaaccga acgccgcgat ctcgggcttg atgatggctc tttctggcct gtactggaag
     9241 gcatcccggc aacagagatg ttcaacgtga ttccactggc accgggtcat gcctacggga
     9301 tgtttatgga acgttttaac gatttatcgg agttacgcaa atgcgcatga atgttttcga
     9361 aatggaaggg tttcttcgcg ggaaatgtgt accgcgagat ctgaaagtga acgaaacaaa
     9421 tgctgagtac ctggtacgta aattcgacgc gcttgaagct aaatgtgcgg cactggaaaa
     9481 caaaataata ccagtgtcag ctgaactgcc accagcaaat gaaagtgttc tgttatttga
     9541 tgctaatgga gaaggctggc tgattggctg gcgttctctc tggtacacct ggggacaaaa
     9601 agaaaccgga gaatggcagt ggacatttca ggtcggggac cttgaaaacg tcaatatcac
     9661 tcactgggca gtaatgccaa aagcaccgga ggctggagca taatgaccac atttaccaat
     9721 aaagaactga ttaaagaaat caaagaacga atcagcagcc tagaggttcg agacgatatt
     9781 gagcgccgtg cttatgaaat cgcactcgta tctctggaag tagagccagt tgaacgcgaa
     9841 gcctatgaat tattcatgga aaagcgtttc ggtgacttag tagatcgtcg gagagcaaaa
     9901 aacggcgata acgaatacat ggcatgggat atgactctcg gttggatcgt ctggcagcaa
     9961 cgagctggta tccatttttc aacaatgaca cagcaagagg tgaaataatg gagccataca
    10021 gcctcacact cgatgaggcc tgtcagtttc ttaagatatc cagaccaacc gccaccaact
    10081 ggatacgaac aggccgccta caggcaacac gtaaagatcc aaccaagcca aaatctcctt
    10141 acctcacaac acggcaagct tgcattgcgg cgcttcagtc tccgctgcat actgtccagg
    10201 tgagcgcggg tgatggcata acagaggaaa gaaaatgtca ctcttccgca gaaatgaaat
    10261 atggtatgcc tcgtattcgc tcccgggcgg gaaacgaatt aaggaatctc ttggcacaaa
    10321 ggacaagcgg caagctcagg agttgcacga caagcgaaaa gcagaactct ggcgagtaga
    10381 aaagctaggg gatttacctg atgtcacttt tgaagaggcc tgcctaagat ggcttgagga
    10441 aaaagctgat aaaaaatctc tcgattcaga taaaagccgg attgagttct ggcttgaaca
    10501 ttttgagggt ataaggctta aagatatctc ggaggcaaag atttactctg ctgtaagcag
    10561 aatgcataac agaaagacga aagaaatatg gaaacagaaa gttcaggccg ccatcaggaa
    10621 aggtaaagaa ccgcctgttt atgaaccaaa gccagtatca actcagacaa aggcaaagca
    10681 tcttgccatg ataaaggcca ttctccgtgc tgcagaacgc gactggaagt ggctggaaaa
    10741 agcgcctgtc atcaagatac cagcggtcag aaacaagcga gtcagatggc tggaaaagga
    10801 ggaagcaaaa cgccttattg atgagtgccc cgaaccactg aaatctgtcg tcaagtttgc
    10861 gctggcaact ggtctgagaa agtcgaacat cataaatctg gaatggcaac aaatcgacat
    10921 gcagcgacga gttgcctggg tgaatccaga agagagcaaa tcaaaccgcg ccattggtgt
    10981 ggcgctgaac gataccgcct gtaaagtgtt gcgtgatcaa ataggcaagc atcacaaatg
    11041 ggtgtttgta cataccaagg cggctaagcg agcagatgga acatcaacgc ctgcggtcag
    11101 gaagatgcgc atcgacagca agacatcatg gctatcagct tgtcgtcgtg caggaattga
    11161 agatttccgt ttccatgacc tcagacacac ctgggcaagc tggctgatcc agtcaggcgt
    11221 cccattatct gtgcttcagg aaatgggcgg atgggagtcc atagaaatgg ttcgtaggta
    11281 tgctcacctt gcgcctaatc atttgacaga gcatgcaagg aaaatagacg acatttttgg
    11341 tgataatgtc ccaaatatgt cccactgtgg aattatggag gatataaaga aggcgtaact
    11401 gattgaattg taatggcgcg ccctgcagga ttcgaacctg cggcccacga cttagaaggt
    11461 cgttgctcta tccaactgag ctaagggcgc gttgataccg caatgcggtg taatcgcgtg
    11521 aattatacgg tcaacccttg ctgagtcaat ggcttttgat ctggttgctg aacaagtgaa
    11581 cgaccgcgtc tgattttctg atttatttcg ctatagcggc aaacaaacac acaccgctgc
    11641 gcgtctgaat caagaaaacc cgtattttca tgtatcaaag tacaatttac cgccctaacg
    11701 gaaaattgtc cgctcctatg agactggtaa ctatgaaacc aacgtcggtg atcattatgg
    11761 atactcatcc tatcatcaga atgtctattg aagttctgtt gcaaaaaaac agtgaattgc
    11821 agattgtcct gaaaacggat gattaccgca taaccatcga ttatctccga acccgtcctg
    11881 ttgatttaat cattatggat atagacttgc ccggaacaga cggttttacc ttcctgaaaa
    11941 ggatcaaaca aatccagagc acagtgaaag tgttattttt atcatcgaaa tcagaatgct
    12001 tttatgctgg cagagcgata caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc
    12061 agaatgatat ttttcatgcc gttcagatga tcctctccgg atacacgttt tttcccagcg
    12121 aaacgcttaa ctatataaaa agcaataaat gtagtacgaa tagttcaacg gtcactgtgc
    12181 tatctaatcg tgaagtgacc atattacgtt atctggttag cggattatct aataaagaaa
    12241 ttgccgataa gttattactt agcaataaaa cagttagtgc gcataaatct aatatttatg
    12301 gcaag
//
LOCUS       DAPGWD010000123        12127 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPGWD010000123 DAPGWD010000000
VERSION     DAPGWD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.124"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            140..574
                     /gene="slyA"
                     /locus_tag="RHX88_003336"
     CDS             140..574
                     /gene="slyA"
                     /locus_tag="RHX88_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDV2231995.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(621..1088)
                     /gene="slyB"
                     /locus_tag="RHX88_003337"
     CDS             complement(621..1088)
                     /gene="slyB"
                     /locus_tag="RHX88_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDV2231996.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1362..2471
                     /gene="anmK"
                     /locus_tag="RHX88_003338"
     CDS             1362..2471
                     /gene="anmK"
                     /locus_tag="RHX88_003338"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDV2231997.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            2569..2898
                     /gene="mliC"
                     /locus_tag="RHX88_003339"
     CDS             2569..2898
                     /gene="mliC"
                     /locus_tag="RHX88_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDV2231998.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2957..3613
                     /gene="pdxH"
                     /locus_tag="RHX88_003340"
     CDS             2957..3613
                     /gene="pdxH"
                     /locus_tag="RHX88_003340"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDV2231999.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRISLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3742..5016
                     /gene="tyrS"
                     /locus_tag="RHX88_003341"
     CDS             3742..5016
                     /gene="tyrS"
                     /locus_tag="RHX88_003341"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDV2232000.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            5075..5938
                     /gene="pdxY"
                     /locus_tag="RHX88_003342"
     CDS             5075..5938
                     /gene="pdxY"
                     /locus_tag="RHX88_003342"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HDV2232001.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5982..6587)
                     /gene="gstA"
                     /locus_tag="RHX88_003343"
     CDS             complement(5982..6587)
                     /gene="gstA"
                     /locus_tag="RHX88_003343"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDV2232002.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(6693..8195)
                     /gene="dtpA"
                     /locus_tag="RHX88_003344"
     CDS             complement(6693..8195)
                     /gene="dtpA"
                     /locus_tag="RHX88_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDV2232003.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(8806..9441)
                     /gene="nth"
                     /locus_tag="RHX88_003345"
     CDS             complement(8806..9441)
                     /gene="nth"
                     /locus_tag="RHX88_003345"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDV2232004.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(9441..10136)
                     /gene="rsxE"
                     /locus_tag="RHX88_003346"
     CDS             complement(9441..10136)
                     /gene="rsxE"
                     /locus_tag="RHX88_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDV2232005.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(10140..10760)
                     /gene="rsxG"
                     /locus_tag="RHX88_003347"
     CDS             complement(10140..10760)
                     /gene="rsxG"
                     /locus_tag="RHX88_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDV2232006.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(10764..11822)
                     /gene="rsxD"
                     /locus_tag="RHX88_003348"
     CDS             complement(10764..11822)
                     /gene="rsxD"
                     /locus_tag="RHX88_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDV2232007.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLTLCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(11823..>12127)
                     /locus_tag="RHX88_003349"
     CDS             complement(11823..>12127)
                     /locus_tag="RHX88_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232008.1"
                     /translation="NAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRK
                     AAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         3109 a         3033 c         3067 g         2918 t
ORIGIN      
        1 aaaaatttac gcgcaatttc tgaaaaatat gtctgacggt aaccaaatgc agcaatacat
       61 ttgttttagc aatacaattg ctgcacacta ttctaaaagc cgcataatat cttagcaagc
      121 taattataag gagatgaaat tggaatcgcc actaggttct gatctggcac ggttggtgcg
      181 catatggcgt gctctgatag accatcgcct gaaaccgctg gagttaacac aaacccattg
      241 ggttacgtta cacaatattc atcagttacc tccagaccag tcgcaaattc aactggcaaa
      301 agcgattggc atcgagcagc catcactggt ccgtactctg gaccaactgg aagaaaaagg
      361 gttaatttcg cgtcaaactt gtgccagcga tcgtcgggct aaacgtatta aactgacgga
      421 aaaggcagag ccactgatca gcgaaatgga agctgttatt aacaaaaccc gcgcggaaat
      481 attacatggc atctccgcag aggaactgga gcaactgatt acgctcatcg caaaacttga
      541 gcataatatc attgagttac aggccaaagg gtgaaatgaa gggggcgtgt ggtcagtaac
      601 ctgaccacac gcaaacttat ttagcgcgga gaaacggtca cctgactgcc attgctggcc
      661 agtacgacac gttggcccgg agagaaacga gtgttgcctt gtttctgtac caccatgatg
      721 gtattaccat cgtctttacg aatttccagc tcgacaccct gcgttttgtt cattgcactc
      781 tgtacgccct gaccagctac gccacctgca acagcgcctg ctgcagtagc cagagaacgc
      841 ccggttccgc caccaacagt attccccagg aaaccaccaa gaacagcacc gccaattgca
      901 ccgataacgt tggaatcatc accgccctga atctgtaccg gacgtacgtt aacgatggtg
      961 ccatagctga cattctgtac ttgtttcgct tcagaagcgg tataaacatc ccctgacagg
     1021 gtgtcgttat taacacaacc gacaagagac agacctacca ttgaaacaac caatacgcgt
     1081 ttaatcattg aaaaatctcc tgttcaccat gaaacgccac gcaagcatcc ctcatggtca
     1141 aagtatatgg catatgtgaa tccatggtta catgttctac caaatatgag caaatcataa
     1201 tgaaagtagg atcaaccaat tataaacaaa acattcatga tgattattga tttgaagata
     1261 atgttttact accaacggta aaaatcggtc attccgcatg gtattcacga aaagtttatg
     1321 tttgagtggt gcgcaaacgt cagattgaaa aaggatgaac tatgaaatcg ggccgcttta
     1381 ttggcgttat gtcaggcacc agccttgatg gtgttgatgt tgtgttggcg acaatagatg
     1441 aacaccgggt cgcacagctg gcaagcttga gctggccgat cccggtgtct ctgaaacagg
     1501 ctgtactgga tatttgccag ggccagcagc ttacactttc gcagtttgga cagcttgata
     1561 ctcaactcgg gcgacttttt gctgatgcgg tcaatgcctt gcttaaggaa caaaacctgc
     1621 aggcgagaga tatagttgcg atcggctgtc acggtcaaac cgtctggcat gaaccgacgg
     1681 gcgtggcacc acacacttta cagattggcg ataacaatca aattgtggca cgcaccggaa
     1741 ttacggttgt cggtgatttt cgccgtcgcg atattgcctt gggaggacaa ggcgcaccgc
     1801 tggtacctgc gttccatcat gccttgctgg ctcactcaac cgagcgacga atggtgctca
     1861 atattggcgg catcgccaat ctgtcactgc tcattcctgg gcagccggtt ggcggctacg
     1921 ataccggtcc tggtaacatg ctgatggatg cctggatctg gcgtcaggcc ggtaaacctt
     1981 acgataaaga tgccgagtgg gcacgggcgg gtaaagttat tctcccactg ctgcaaaata
     2041 tgctcagcga cccatatttc tcgcaacctg caccgaaaag caccggacgc gaatacttta
     2101 actacggctg gctggagcgc catttacgcc attttccggg tgttgatccc cgtgatgttc
     2161 aggccacact ggcagaactc accgccgtga ccatttctga acaagttttg ttgagcggtg
     2221 gctgcgaacg attgatggta tgtggtggag gtagtcgtaa tccgctactc atggcgcgtc
     2281 tggcggcatt actgccaggc acagaagtca ccaccaccga tgccgttggc attagtggcg
     2341 atgacatgga agcattggct ttcgcctggc ttgcctggcg gacgctggcg ggattaccag
     2401 gaaatctgcc ttccgtcact ggcgcaagcc aggagacggt actgggggct attttccctg
     2461 ctaacccgtg atatcagagt taactgaatt tttcttaccg cgtctgccgt tacacttgga
     2521 acgttaggga gggcgtattg ccctccagac caggaaagtc ttcgggaaat gtctatgaaa
     2581 aaactgttac tgatctgttt gccggtgctt ctcaccggct gtagcgcgtt caatcaactg
     2641 gttgagcaca tgcaaaccgc tacgctggaa taccagtgtg atgaaaaacc gttgacggtc
     2701 aaactgaata atccgcgcca ggaagtcagt tttgtttacg ataatcaact actgcatctc
     2761 aaacagggca tttcagcgtc tggtgcacgt tacactgacg gaatctatgt tttctggtcg
     2821 aaaggcgatg aagcgactgt ctataaacgc gaccgcatcg tattgaataa ctgtcagtta
     2881 caaaatccac agcgttgaga tttttccagg ggcggcgcac aatagcgtca cccactgaca
     2941 atccgtaaag aaaaccatgt ctgataacga cgaattgcag caaatcgcgc atctgcgccg
     3001 tgaatacacc aaaggcgggt tacgccgtcg cgatcttccc gccgatccat taaccctttt
     3061 tgaacgttgg ctctctcagg cgtgtgaagc caaactggca gaccctaccg cgatggtggt
     3121 cgctaccgtg gatgaacatg ctcagcctta tcaacgcatc gttttactca aacattacga
     3181 cgaaaaaggc atggtgtttt acaccaacct cggtagccgt aaagcacatc aaattgaaaa
     3241 taatccgcgc gttagcctgc tgttcccgtg gcataccctt gaacgccagg tgatggtgat
     3301 cggtaaagca gaacgacttt cgactctcga agtgatgaaa tattttcata gccgcccgcg
     3361 tgatagccag attggtgcat gggtttcgaa gcagtccagt cgcatttctg cccgcggtat
     3421 ccttgaaagt aaattcctgg agctgaagca gaagtttcaa cagggcgaag tgccattgcc
     3481 gagcttttgg ggcggttttc gcatcagcct tgaacagatt gagttctggc agggcggtga
     3541 gcatcgcctg catgaccgct ttttgtacca gcgagaaaat gatgcgtgga agattgatcg
     3601 tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg ctggtactcc tgattctggc
     3661 actttattct atgtctcttt cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc
     3721 gttatataca tggagatttt gatggcaagc agtaacttga ttaaacaatt gcaagagcgg
     3781 gggctggtag cccaggtgac ggacgaggaa gcgttagcag agcgactggc gcaaggcccg
     3841 atcgcgctct attgcggctt cgatcctacc gctgacagct tgcatttggg gcatcttgtt
     3901 ccattgttat gcctgaaacg cttccagcag gcgggccaca agccggttgc gctggtaggc
     3961 ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg ccgagcgtaa gctgaacacc
     4021 gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc aggttgcccc gttcctcgat
     4081 ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact atgactggtt cggcaatatg
     4141 aatgtgctga ccttcctgcg cgatattggc aaacacttct ccgttaacca gatgatcaac
     4201 aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg ggatttcgtt cactgagttt
     4261 tcctacaacc tgttgcaggg ttatgacttc gcctgtctga acaaacagta cggtgtggtg
     4321 ctgcaaattg gtggttctga ccagtggggt aacatcactt ctggtatcga cctgacccgt
     4381 cgtctgcatc agaatcaggt gtttggcctg accgttccgc tgatcactaa agcagatggc
     4441 accaaatttg gtaaaactga aggcggcgca gtctggttgg atccgaagaa aaccagcccg
     4501 tacaaattct accagttctg gatcaacact gcggatgccg acgtttaccg cttcctgaag
     4561 ttcttcacct ttatgagcat tgaagagatc aacgccctgg aagaagaaga taaaaacagc
     4621 ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg tgactcgtct ggttcacggt
     4681 gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc tgttcagcgg ttctttgagt
     4741 gcgctgagtg aagcggactt cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg
     4801 gaaaagggcg cagacctgat gcaggcgctg gtcgattctg aactgcaacc ttcccgtggt
     4861 caggcgcgta aaactatcgc ctccaatgcc atcaccatta acggtgaaaa acagtccgat
     4921 cctgaatact tctttaaaga agaagatcgt ctgtttggtc gttttacctt actgcgtcgc
     4981 ggcaaaaaga attactgtct gatttgctgg aaataatgca ttaagtggaa agggggagtg
     5041 agaaatcact ccccctggtt tttatacagg gaacatgatg aaaaatattc tcgctatcca
     5101 gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca gagtttccta tgcgccgcct
     5161 gggcgcgaac gtctggccgc tgaacaccgt tcaattttct aatcacaccc aatacggcaa
     5221 atggactggc tgcgtgatgc cgcccagcca tttaactgaa attgtgcaag gcattgccgc
     5281 cattgataaa ttacacacct gtgatgccgt gttaagtggc tatctgggat cggcggagca
     5341 gggtgaacat atcctcggta tcgtccgtca ggtgaaagcc gcgaatccgc aggcgaaata
     5401 tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt atcgttgcac cgggtgtcgc
     5461 agagtttcat gtgcggcacg gtttgcctgc cagcgatatc attgcgccga atctggttga
     5521 gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa gaagcggttc tggcagcgcg
     5581 cgaactcatt gcgcaagggc cacaaattgt gttggttaaa cacctggcgc gagctggcta
     5641 cagccgtgac cgttttgaaa tgctgctggt caccgccgat gaagcctggc atatcagccg
     5701 tccgctggtg gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga cgagcggttt
     5761 actgctggtg aaactgcttc agggggcaac gctgcaggag gcgctggaac atgtgaccgc
     5821 tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag gaatatgagc tgcaagtggt
     5881 ggctgctcag gatcgtattg ccaaaccaga acattacttc agcgcaacaa agctctgaaa
     5941 tatctcattt cggcccacaa cgacctgtgg gccgatggat attactttaa gccttccgct
     6001 gacagcgcgt cttgtacttc cggacgttca gccatacgtt gcataaatgc tgcaatgtgc
     6061 tctaaccctt ccagattcag tttcaccgcg tatgcccagc gcagaaccgt aaacagatag
     6121 gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct catccttcag tgcctcgttc
     6181 acatattgca gcttcttctc cagctgcgcg cgaactgtcg gtttgtactc ttccggtgta
     6241 tcagggcgaa atagaggtgt gaaaccttta tgcagctcgg tggcgatgta attcagccat
     6301 tcgatggttt tatagcggga aatactgttt accggtgcca gcaactggcg gtcggggacg
     6361 ctgtcggcaa gatactgcat aatcgctacg ccttccgtca gcaaagtacc gtcatccagc
     6421 agcaatgcag gcacctgccc cttagggtta acggcaaagt aatcgtcacc gttttcgaga
     6481 cgttttttca ttaaatccac actgacgagg gtaaaatcct ttccgctctc acgcagggtg
     6541 atatgggaag cgagagagca ggcacccggt ttgtagaaca atttcatcgg taactccttt
     6601 tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg ctaacgaaaa
     6661 gttagcggct tgtaaaagag tttccctgaa ttttacgcta cagcagcttt cgccgctttg
     6721 tctgcagcgt catcctgcgt catacggtgc agtttaggcg cggtcagcag catcagtact
     6781 gcaataatgg cagtagcgac accaatctgc aagaatacgc gaccataaac ttccagtgac
     6841 atcagcgggt cggtaacgtt atccggcaca gccatcatac ccgcaacata accaccaatc
     6901 aggtttgcac cggcagtggt caggaaccag ctacccataa tgaagcccat cagacgctgc
     6961 ggaacgagtt gtgcaaccat tgccagaccc agaccagaga tcatcagttc cccgatgctc
     7021 tgcaggccat agcttgcgac cagccagctt acagacacga taccagcgtc agacgcgaat
     7081 ttcgcaccca gcggcagaat caggaacgca ccagaacaca tcaccatgcc gattgcaaac
     7141 ttggttggca tcggcagggt atcgcccatc ttgttataga tagcggccag aatcggacta
     7201 ccgatgatga tccagaacgg gttcagtgcc tgatactgct caggttctac ggccagaccc
     7261 agaatggagt gctcaacgtt acgaatcgca aagaagttca gtgacgttgg catctggctg
     7321 tacagcacga agaagataat ggcttcgagc atcaggatga aggcgacgat cattttacga
     7381 cgcgcagcac ctttcatcgc gaaggcttct ttaccgaaga taaccacgat accgaaggca
     7441 acaacgccca gcgccatacg cgcaacttcc tgattgtgca gcagccaggt ggcgatagcg
     7501 atcagtgcca caacaccaat aatggtcagc agcaggttac ggtagttgat aggctcgaag
     7561 tctggttttg aaccgtactg tttaacccag cgttggcaga aggcgaagtt aacgatagtg
     7621 atcagcaggc ctacaacgct caacgcaaac gcaacactcc agccgtattt cgcggccagc
     7681 cacggcgtag caatcataga gaagaaagag ccgatgttga cggacatgta gtacatggtg
     7741 aatgcaccgt ccagacgcgg gtcgtttttc tcatagcatg tagaaagcag agaagacggg
     7801 ttagctttaa acaggccgtt accgaccgca atagccgcca tacccatata aacgataccg
     7861 gcgtcgtgac cagaccaggc aaccagagca taaccaatcg ccagcacaat agcgccgagc
     7921 ataattacgc gtttagtacc cagtaccttg tcacctaacc agccgccgat agcgaccaga
     7981 ccataaacca gggcactaaa ggaagagaaa agggtgattg aatccgcttc agacataccc
     8041 agttgtttaa ccaggtaaac agccataatt ccttgtaggc cgtaataacc aaaacgttcc
     8101 cataactcaa tcgagaagat gagatagaac gccttcggtt gtttgaaagc gttcaaactg
     8161 acgctttcag ttggtttttg gtttgcagtg gacactttta cctctttttt tacatcccat
     8221 attaacgggg gtgctttctc gcgttatgtc taaaaaacat acgcgcaatt gttatagtgg
     8281 gaggggaaac ggcaggtaat gttcactatc ttgcactttc agacaagtcc tttgtaataa
     8341 tctgttacat ataactgagg cggggaattc aaccattccg ggaaatgtta tccagcgtta
     8401 aatttttcaa aaaacgtaac ggcagatttt ttcactgcac tgagaggggg gtgatggctt
     8461 aacggcgata tgttctgcta tttagcgttt ttactagtga gatagtccag tttctgaaaa
     8521 atagccagtg taatgttttg taggtcaata tttgttgcgt gtgtaaattt aaatgatttt
     8581 aacctgattc ttgcaatgtt tttacattgc gacagcatcg tgatcttgat cacgaatgaa
     8641 tagaaaattt cgctattaaa aaaacaatta acctgcattt ttggttatcc gcaaacggat
     8701 tacctgatga taacgctaag caatggcatc attcacgtta aaaagaagtt ttaaaagagg
     8761 ataaagaaag gttatcaatg gggtaatcgg tgttacccct tttcttcaga tgtcaacttt
     8821 ctctttgtat tcacaaagat cttcaataat acaagagcca cagcggggct tgcgggcaat
     8881 gcaggtataa cgcccgtgca ggatcaacca atggtggcag tcgactttaa actctgccgg
     8941 aaccactttc agtagctttt cttctacctg ttcgacgttt ttccccggcg caaattgagt
     9001 acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc cgaatgcagt
     9061 gtttaatacg acgttggctg ttttacgacc tacgccgggc agggcttcaa gcgcagcacg
     9121 atcttccgga acctcgccat tatgctgctc cagcaagata cggcaggttt tgatgatatt
     9181 ttctgctttg ctgttataaa gcccaatcgt tttgatatag gttttcaccc cttcaacgcc
     9241 cagttcaagc atcgctgcag gcgtattcgc caccgggtag agtttcgccg tcgccttatt
     9301 aacactgaca tcggtcgcct gagcggaaag cagtacggca atcagcaatt caaaaggcga
     9361 actgaaatta agctcggtgg tgggatgagg attgttctca cgcaggcgag tgaggatctc
     9421 caggcgtttt gctttgttca tcagacattc cctgtttcac cgtttggcaa tgcacgttcg
     9481 gcagctgctt cagtacggcg ctttttcatt ctttcatcaa tcaggtattt tcctgccagc
     9541 atcagtccca ggccaataaa tgcacctgga ggcagcatcg ccagcaggaa aggggagtcg
     9601 gtgtggaaaa tttccacgcg taatactttt gcccagctac ctaacagcgc atctgcaccg
     9661 tcaaacaatg tgccattgcc gataatttcg cgtagtgaac ccagcacgaa catggcgcag
     9721 gttgcgccca taccaattga aaagccgtcc agtgccgaaa gcgccggacc ttttttggcg
     9781 gcgaaggctt cagcgcggcc cacaacgata cagttagtga caatcagcgg aataaaaatc
     9841 cctaatgatt gatacaggcc aaaggcgtag gcgttgatca gcatctgtac agcgctgacc
     9901 accgaggcga tgatcatcac gtaaatggga atgcggatct cggctggcgt ccagtgacgc
     9961 agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg tagtcgcaag tcctaaaccc
    10021 agagcgttag tggcagtgga cgtgaccgcc aacagaggac aaaggccgag caactggacc
    10081 agcgcagagt tgtttttcca caacccctga acaataacgt ctttaatttc gctcacgctt
    10141 tattctccac aggcaggaag ttgagaaagt tgtgccggta acgtctgagc gtacaatccg
    10201 gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg tcgcgccggt gaactggtcg
    10261 aaatcaccac catctttctt caccgcccag tgcgcatcat ctgcaccact gattttttta
    10321 cccgcaaaat gggtgatcca gtcagaaagg cgcagttcga ttttatcgcc aagccctggc
    10381 gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc cgttaaaatc ggctcccacc
    10441 agcagctgaa tcgcaccgga atagccatct ggcgcggttg cttccagaac ggcggctacc
    10501 ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac ctttacctaa ctctggcgca
    10561 gttaccagat agcaactctg tgccagcgca ttgttatagc gttcggctgg cagcacctga
    10621 tcaaataatg ccttttgttg cagactggcc tgttcagcaa tcgtcgtttt ggtcatctgg
    10681 ttgatggccg cagttaaccc tgttgaaccc gctgcaaata gcgctaacgt aatgccgtgt
    10741 tttcggatag ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg cggacgcgtg
    10801 tagtaatcga tcagaggaac cgtgatgttc gccagcagga cggcaaaagc cacgccgtca
    10861 ggatagccgc cgaaactgcg gatcaaccag actaataagc ccgccagcgc gccgaaaata
    10921 agacgaccac gattggtcgt agaagcggta accgggtcag tcaaaataaa gaatgcgccg
    10981 agcatggtcg ctccagacag cagatgaatt tgcggtgctg ccagtgtttc tggtgagaac
    11041 aaccagccca acgttgcgca taacgtcagc gttactaaga agctgagggg aatatgccag
    11101 cgaatcgctt tctgccatag caaccacacg ccgccagcca gccaggcgag atttacccat
    11161 tgccaaccag cgcccgccag aataccgcta tagatcggat attgcataat ctgttcaacc
    11221 gaatgaccgg cacggacaga ggttttaaat gtatccagcg gtgtagcctg actaatgcca
    11281 tcaataccta agcgtagtgt gttcatatca ccaccactgg cggtatgccc gctgaaaata
    11341 acctgtatgg cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg cggtaaccag
    11401 ctggtcatct gcaccgggaa ggagatcagt aagaccacat aaccaatcat tgccggatta
    11461 aacgggtttt gccccagacc gccatacaac tgtttagcga tgatcaccgc aaacaccgta
    11521 cccagcacga ccatccacca tggcgcgagg gggggaatac ttaccgccag caataagcct
    11581 gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg actgcttgcg tagtttgagt
    11641 acgagagctt cggctaacag agcactaacc gatgccaaca ggatctgaac gagagtaccc
    11701 caaccaaaaa accacagttg cgctgcgatt cctggcacgg ctgcgagcaa caccaacagc
    11761 ataatgcgcg atgtctggcg ctggttatgg gtataagggg agctagctat tctgaatacc
    11821 atttagtcct cgtttacaac cttctgctgg gcggcttttt tggcctgagc gcgggcaata
    11881 gccgcggcaa ctgccgcttt gcgcggatca acctgttctt ctggttctgc gttagtctgt
    11941 tgctgttcca gcttgcgcgc tttggcacgg gcaatagcgg cttcgacggc ggctttgcgc
    12001 ggatcaacct gttcttctgg ttccgcatta gcctgttgct gttccagttt gcgcgctttg
    12061 gctcgggcaa tagcggcttc gacggcggct ttgcgcggat caacctgttc ttctggttcc
    12121 gcattag
//
LOCUS       DAPGWD010000124        12026 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPGWD010000124 DAPGWD010000000
VERSION     DAPGWD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.125"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            200..970
                     /locus_tag="RHX88_003350"
     CDS             200..970
                     /locus_tag="RHX88_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDV2232009.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            961..1434
                     /locus_tag="RHX88_003351"
     CDS             961..1434
                     /locus_tag="RHX88_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDV2232010.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1541..2080
                     /gene="dnaT"
                     /locus_tag="RHX88_003352"
     CDS             1541..2080
                     /gene="dnaT"
                     /locus_tag="RHX88_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDV2232011.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2083..2820
                     /gene="dnaC"
                     /locus_tag="RHX88_003353"
     CDS             2083..2820
                     /gene="dnaC"
                     /locus_tag="RHX88_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDV2232012.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2869..3363
                     /locus_tag="RHX88_003354"
     CDS             2869..3363
                     /locus_tag="RHX88_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDV2232013.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3617..5908
                     /gene="opgB"
                     /locus_tag="RHX88_003355"
     CDS             3617..5908
                     /gene="opgB"
                     /locus_tag="RHX88_003355"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDV2232014.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSSQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6048..7070)
                     /gene="lgoD"
                     /locus_tag="RHX88_003356"
     CDS             complement(6048..7070)
                     /gene="lgoD"
                     /locus_tag="RHX88_003356"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDV2232015.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7209..8123
                     /locus_tag="RHX88_003357"
     CDS             7209..8123
                     /locus_tag="RHX88_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2232016.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8338..9699
                     /locus_tag="RHX88_003358"
     CDS             8338..9699
                     /locus_tag="RHX88_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2232017.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSCKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9748..11412)
                     /gene="tsr"
                     /locus_tag="RHX88_003359"
     CDS             complement(9748..11412)
                     /gene="tsr"
                     /locus_tag="RHX88_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDV2232018.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMVVADSEENWETF"
     gene            complement(11531..11977)
                     /gene="hpaR"
                     /locus_tag="RHX88_003360"
     CDS             complement(11531..11977)
                     /gene="hpaR"
                     /locus_tag="RHX88_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HDV2232019.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         2917 a         3179 c         2936 g         2994 t
ORIGIN      
        1 cattatagtg ataatattca tgtgatagtt tgtttgcggc gagagataat tcgcttttta
       61 tcaccgagct tgtcccgata accaggatta atcagcgcaa ttttgatctt aacctaccgc
      121 aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata cgcagcacgt
      181 ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacac ggctttgtat
      241 ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg atgagctttc
      301 ctcacgactg ggtcgggcgc tgggaatgga cagtgtcgaa agttctatct cttcgaacgc
      361 catagtgctg acaactatta aagatgggca atgcctgaca tcgacacgta aaaatcacga
      421 tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc ttgcggagca
      481 tcatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc cattacgtta
      541 cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct gtaaactcaa
      601 taacggtggc tgggatggtg ccgtcatcac cttctttgcc agtacgaccg cgatgtatat
      661 ccgccagctg ctggcacaac gtcatcttca tccacagatc aacttttgcc ttaccgcttt
      721 cgccgccacc accatttccg gattgctttt gcaactcccc actttcagca atacccccac
      781 cattgcgatg gccgccagcg ttctgctgct ggtgccgggc tttccgttga ttaatgccgt
      841 cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga tcgccagtct
      901 gctgacactg gctacctgcg tcggcgtggt gatggcactg acgatttggg ggctacgcgg
      961 atgggtgtga tcgaatttct gtttgcattg gcgcaggata tgatcctcgc cgccattcct
     1021 gcggtcggct ttgcgatggt gttcaacgtt cccgtgcggg cgttacgctg gtgtgcgctg
     1081 cttggctcga taggtcatgg ttcccgaatg atcttgatga ccagcgggtt gaatattgag
     1141 tggtcaacct ttatggcttc tatgctggtc ggcaccattg gtattcaatg gtcgcgctgg
     1201 tatctggcgc atccgaaagt gtttaccgtg gcggccgtta tccctatgtt cccgggcata
     1261 tcggcttata ccgcaatgat ttcggcggta aaaatcagcc agctaggtta cagcgaaccg
     1321 ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg tgcgttatcc
     1381 atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt ataaaattgc
     1441 ctcgcttaag cacacggatg agagacagcc tcctctcctc cgtgtgttac tataaaagtt
     1501 atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca gagttttgac
     1561 cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc tggcaaaagc
     1621 tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg ccgtcacgcc
     1681 tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg gaagcgatgt
     1741 cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg tacccatggg
     1801 gaaattcgcc atgtatccgg actggcaacc cgatgccgac tttatccgcc tggcggcgct
     1861 atggggcgtg gcgctaagag agccggtaac caccgaagaa ctggcctcat tcattgccta
     1921 ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac tggcgcgcag
     1981 cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga atacggtcag
     2041 tgaacctgac agccaaattc caccaggatt cagagggtaa cgatgaaaaa cgttggcgac
     2101 ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacccgcatt caaaacgggt
     2161 gaagaactac tggcctggca aaaagaacaa ggggcgatcc gctccgccgc tctcgaacgt
     2221 gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg tccactgcat
     2281 cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa tgcgttaagc
     2341 aaagcgcgcc agtatgtcga agagtttgac ggcaacatcg ccagctttat cttttctggt
     2401 aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct gctgctacgc
     2461 ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa agataccttc
     2521 aggaatagcg gtaccagcga agaacaactg cttaacgatc tgagcaacgt tgatctgctg
     2581 gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat catcaaccag
     2641 atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa cagtaatatg
     2701 gaagagatga caaagttgct gggcgaacgc gtgatggacc gtatgcgcct gggtaacagt
     2761 ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacgggtaa agagtattaa
     2821 tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat gatgaaaact
     2881 attaaacacc ttctgtgctg tgccattgcc gccagcgcat taatttccac cggggtgcat
     2941 gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa ccaaaacagc
     3001 acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag cagcggaaac
     3061 caggccttaa gcgcagataa catgaacaat gccgcaggca ttctgcaata ctgcgcgaag
     3121 caaaagctgg cttcggtaac cgatgccgaa aacatcaaga accaggtgct ggaaaagctg
     3181 ggcctgaaca gtgaagagca aaaagaagac accaactatc tggacggtat tcagggtttg
     3241 ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacaac tccgctggca
     3301 gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa cttcatttcc
     3361 tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc tcctgccctt
     3421 ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga tcaaaaaagt
     3481 ctgtttataa actaattcct aacaacatcg catttttttg acactagaat tgcagcaata
     3541 tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa cggcccggtt
     3601 tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg cctctgtgct
     3661 gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat taacggtgct
     3721 ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta ctggcgatgg
     3781 tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg gcgtcagcaa
     3841 atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg gtgcgctggg
     3901 ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc tgctggcgct
     3961 cttactggcg ctgggttcgg tggacgccag cccagcattt cgtcagataa cggaactggt
     4021 gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag agccgtcgaa
     4081 aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc tcgagcggac
     4141 ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga
     4201 aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga ttgcgggcat
     4261 ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg cctccgcctc
     4321 tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa actcgggtta
     4381 tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg tgttcctgaa
     4441 gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg tggccgaccc
     4501 gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag cgtggaaaaa
     4561 gtttgaagag ctttcccgct caagtcagcg attctcactg tttaccctga cagtcgatac
     4621 ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg attttgacgg
     4681 taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg cggcgtttat
     4741 caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct cttctgacca
     4801 tttagcgatg aacaacacgg cgtggaaata ccttaataag caggatcgca ataacctgtt
     4861 ttttgtcatc cgtggcgaca agccgcagca agagacgctg gcagtgaagc gtaacacgat
     4921 ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg gacttggtcg
     4981 tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag agaaaacatt
     5041 ggcgtggaag ccagatatca tccgcctgtg gaaattccct aaagagatga aagagttcac
     5101 catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt tgccgctgct
     5161 gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact cagcaccgct
     5221 gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg accgttgcta
     5281 taagatggca caactctggg ctccggaact ggcactctcc accgactggt gtgtctcgca
     5341 agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat ggcagggcaa
     5401 aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg tcgatacgct
     5461 gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca acgtcgccgg
     5521 tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt ggggccgctg
     5581 gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa
     5641 atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc ctattccggt
     5701 acgcgtaggc aatgaagaac aaactcttgt gctgggcaat gaagtgacca ccaccacact
     5761 gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg aacctgtctc
     5821 aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg gcatggtgga
     5881 aattaaagtt gtggaacgtg aagggtaatt tctgactgat gtgtaggtcg cataaggtgc
     5941 gacctacaca tcttctccgg cctacaaaac atcattgaga aacagaaaaa aaacagcgcc
     6001 caacacttcc ccggacgctg catggcaact ttaataatct cgccttatca gaaagtaatt
     6061 acacccttaa tcaactcacg attgttaatc acatcgcgct cgtaggtttc tgccagcgtg
     6121 gcgaacggat agcgatgggt taacatcatg tcggcagtga tttttccttc cgccatcagt
     6181 cgaccgactt tagcaaaatc ttccggcgtg gcgttgcggc tgcccatcat cgtcgtttct
     6241 tttttatgga attccggatc ggagaactgc aactcacctt taaacaggcc gacaaatacc
     6301 accgtgccgc cgtgacgaat caaattcacg gtgttattca tcgcatgttg attacctgtc
     6361 gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg cccgtagctg cgcgtcaaaa
     6421 tcttcggctg acgggtccag taaaggtaat tccagacgcg ttgccacatg ttcacggcgc
     6481 gccggactgg tatccgccac caccacctgt gcgccatcgg ctttagcgat tgccgccgcg
     6541 cccagaccga ttggccccgc cccgaccacc agcacctgct cgccgggagc aatggctgcg
     6601 cgacgcaccg catgagcgct aatagcgaaa ggttcaatca atgcggccgc ctgcgggtca
     6661 ataccgtctg cgggcaaaat gttcgccacc ggcacgctca aatactcact aaaaccgcca
     6721 tcctgatgca cgccaatgac tgaaattttt tcacagcaat tggtacgccc gcttttacac
     6781 gccgggcatt gctgacaggc aacataaggg atcacagcaa cttgctgacc atttttaaga
     6841 tcagcaatat ttttacccag cccaacaatc tccccacata tttcatggcc taaaacacgt
     6901 ggataactaa aaaatggttg atttccaccc caggcatgaa tatcggtacc gcaaatcccg
     6961 acagacttaa tttttattaa tgcttcattg tcacccggaa taggtatctc gcgttgtttc
     7021 cagactaatt ctttcggctg ctggcaaatt aaaacattca tcgtagacat gattcggctc
     7081 tccatttttt gttgccttag atatcaatga aaatgaaaaa tggcagcgga acggaaaatc
     7141 tgttttgtga aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg actcacaagg
     7201 aggaaaatat gagtcgttca caaaatttac gccacaatgt gattaaccag gtcattgatg
     7261 atatggcgcg gggtcatatc ccttccccac ttccatcgca aagtgcgctg gcggaaatgt
     7321 acaacatcag ccgcaccaca gtgcgtcata ttctcagcca tttacgcgaa tgcggcgtct
     7381 tgacgcaggt tggaaacgac tatgttattg cgcgtaaacc tgaccacgat gacggtttcg
     7441 cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt tgagcaggct tttttcacga
     7501 tgataaacca gcgccagtta cgcccagggg aaactttttc tgaactgcaa ctggctcggg
     7561 ccgcgggtgt cagcccggtg gttgtaagag aatatctttt aaaattcggc cgttataatc
     7621 ttattcaaag cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa tcctacgccg
     7681 agcagctgtt tgagctacgc gaaatgctcg aaacacattc acttcagcac tttctcaacc
     7741 tgcctgatca tgatccccgc tggctgcagg cgaaaaccat gctcgaacgt catcggctac
     7801 tgcgtgacaa catcggcaat agctttcgca tgttctcgca acttgacaga gattttcact
     7861 ccttactgct ttcagctgcc gacaatatct tttttgatca atcgcttgag atcatctcag
     7921 tcatctttca ttttcactat caatgggacg aaagcgatct taaacagcgc aatatcattg
     7981 ccgtcgatga gcatatgacc attctcagcg ctctgatttg ccgcagtgac cttgatgcca
     8041 cccttgcgct ccgtaaccat ttgaattcag ctaaacaatc aatgattcga tccattaatg
     8101 agaacacgcg ttatgctcat taagtacgga ttaaaaacag caaactatta gcgggtgcac
     8161 ggttttatca aaagataaat accgggaaat tcccgcttac ctatgctcac aatcagtcga
     8221 ctaaaaatgt ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc gttaatgtca
     8281 accttgcgta atgcacaatt acgtaaacaa ttaaaattat aaatacagga gtctggcgtg
     8341 gaaaaagaaa atatcaccct cgatccgcgt tcttcattta ctccatcttc gtcggcagat
     8401 attcccgtgc caccagatgg attagttcaa cgcagtaccc gaattaaacg cattcaaacc
     8461 accgccatgt tgttattatt ttttgcggcg gtaatcaatt atctcgaccg cagttcgctg
     8521 tcggtagcaa atttaacgat tcgtgaagaa ttgggattaa gtgccaccga aatcggcgct
     8581 ttgctctccg tgttttcact cgcttacggg attgcgcaac ttccttgcgg cccactattg
     8641 gatcgtaaag gcccacgcct gatgctggga ctggggatgt tcttctggtc actgttccag
     8701 gcaatgtctg gcatggtgca cagctttacg cagttcgtgt tggtgcgtat cggtatgggg
     8761 attggtgaag cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg gttcaacatc
     8821 aaagagcgcg gacgcccgat gggcttcttc aacgcagctt ctaccattgg cgttgccgta
     8881 agcccaccga ttctggcggc gatgatgctg gtgatgggct ggcgcgggat gtttattacc
     8941 attggtgtac tggggatttt tctcgccatc ggctggtata tgctctatcg caaccgcgag
     9001 cacgtagaac tgactgccgt tgaacaagct tatctcaatg caggtagcgt caatgcccgc
     9061 cgagatccgc tcagttttgc cgaatggcgc agcctgttcc gtaaccgtac aatgtgggga
     9121 atgatgctcg gattcagtgg catcaactac actgcgtggc tgtatctggc ctggcttcct
     9181 ggttacctgc aaacagccta taacctggat ttaaaaagca cagggttgat ggcggctatc
     9241 cctttcctgt ttggggctgc cgggatgctg gtcaacggtt acgttactga ctggctggtc
     9301 aaagggggaa tggctccgat taaaagctgt aagatctgca ttattgccgg gatgttctgt
     9361 tctgccgcct ttacgctggt cgtaccacaa gcgacaacat ccatgacggc ggttctgctg
     9421 attggcatgg cactgttctg tattcacttt gccggaacat cctgctgggg cttgatccac
     9481 gtcgcagttg cttctcgcat gactgcgtcg gtgggcagta tccagaactt tgccagcttc
     9541 atctgcgcct cttttgcgcc gatcattact ggttttattg ttgataccac ccactcattc
     9601 cgtctggcac taatcatctg cggttgcgtc accgcagcgg gggcactggc gtacatcttc
     9661 ctggttcgtc agccgatcaa cgacccacgg aaagattaat ctccctatgc ccgataacat
     9721 tttgcttatc gggcattttt atggcgatta aaacgtttcc cagttctcct cgctatctgc
     9781 cacgaccatt ttacgcggcg cagctggcgt cacggttttt accacagccg atgtttcacg
     9841 ctgctgttgc tgctgttgct gaatccggaa cactgccaca gcttcggtca ggcgactggc
     9901 ctgctcttcc agcgcggcgg cggcagcggc agactcctcc accagcgcag cgttctgttg
     9961 agttacccgg tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc
    10021 atcagaagca gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat
    10081 ctccgccatt gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac
    10141 cgagtcttca atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt
    10201 acgcacttct cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc
    10261 aaccgccgcg ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat
    10321 aatatcggcg attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc
    10381 taccactttg ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg
    10441 gcgcgcattc tcggcgttct gtttcaccgt tgcggtcagt tgctccatgc tggctgccgt
    10501 ctcttccagc gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtagc
    10561 gatttcgctg gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg
    10621 catcagctct ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc
    10681 atccacctca atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag
    10741 gcgattcatt ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc
    10801 cagtacgacg atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt
    10861 atcgctgacg gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt
    10921 ctcgaaaccg tcttgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc
    10981 acctaacagt tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat
    11041 ctctgccgct gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca
    11101 gtttttttcc gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga
    11161 accgctacca atattattct gatccatcat gtagcggata cccgcgcggt tgagggtgtt
    11221 acgcgtctgc aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt
    11281 ttgtaaaaca gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga
    11341 tgtcagttgt aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat
    11401 acgttttaac atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca
    11461 aaaactttat gaaaaggtgt aacccgccat ccatggaaaa accaggcgct ggttatcaga
    11521 tactaaaaag ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa
    11581 taaattcctc taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa
    11641 tctgccgata ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt
    11701 tggttagcga gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac
    11761 cgtcgcgttc catccgcgta aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat
    11821 acgccagatc gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact
    11881 gttgctcagt caaattatgc cgtttaacga ttgggcgaaa ataactcatt gccgcttctc
    11941 gcgcctggag caacgcaatg gttagagagt cgtgcattat ctttcccctg aaaaattcaa
    12001 aatcattaat atagaaacag ttaatc
//
LOCUS       DAPGWD010000125        12009 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPGWD010000125 DAPGWD010000000
VERSION     DAPGWD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.126"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..605
                     /locus_tag="RHX88_003361"
     CDS             3..605
                     /locus_tag="RHX88_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDV2232020.1"
                     /translation="MNINYPAEYEIGDIAFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(607..1128)
                     /gene="ruvC"
                     /locus_tag="RHX88_003362"
     CDS             complement(607..1128)
                     /gene="ruvC"
                     /locus_tag="RHX88_003362"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDV2232021.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1163..1903)
                     /locus_tag="RHX88_003363"
     CDS             complement(1163..1903)
                     /locus_tag="RHX88_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2232022.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1932..2384)
                     /gene="nudB"
                     /locus_tag="RHX88_003364"
     CDS             complement(1932..2384)
                     /gene="nudB"
                     /locus_tag="RHX88_003364"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDV2232023.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2502..4274)
                     /gene="aspS"
                     /locus_tag="RHX88_003365"
     CDS             complement(2502..4274)
                     /gene="aspS"
                     /locus_tag="RHX88_003365"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDV2232024.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4584..5150
                     /locus_tag="RHX88_003366"
     CDS             4584..5150
                     /locus_tag="RHX88_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV2232025.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTAAEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5147..5965
                     /locus_tag="RHX88_003367"
     CDS             5147..5965
                     /locus_tag="RHX88_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDV2232026.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6018..6413
                     /locus_tag="RHX88_003368"
     CDS             6018..6413
                     /locus_tag="RHX88_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDV2232027.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6454..7197
                     /gene="cmoA"
                     /locus_tag="RHX88_003369"
     CDS             6454..7197
                     /gene="cmoA"
                     /locus_tag="RHX88_003369"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDV2232028.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7194..8165
                     /gene="cmoB"
                     /locus_tag="RHX88_003370"
     CDS             7194..8165
                     /gene="cmoB"
                     /locus_tag="RHX88_003370"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDV2232029.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8330..10759)
                     /gene="torZ"
                     /locus_tag="RHX88_003371"
     CDS             complement(8330..10759)
                     /gene="torZ"
                     /locus_tag="RHX88_003371"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDV2232030.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10784..11884)
                     /locus_tag="RHX88_003372"
                     /pseudo
     CDS             complement(10784..11884)
                     /locus_tag="RHX88_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
BASE COUNT         2754 a         3204 c         2921 g         3130 t
ORIGIN      
        1 acgtgaatat taattaccct gctgaatatg aaattggaga tatcgccttt acatgtatag
       61 gtgctgcctt atttggtcaa atatcagctg catcaaattg ttggagtaat cacgtcggga
      121 tcattatcgg tcataacggt gaagacttcc tggttgcaga aagccgggtt cccctctcaa
      181 ccatcactac gctatcccgt tttattaaac gctctgctaa tcaacgctat gctataaagc
      241 gattagacgc tgggctgaca gaacaacaaa aacaacgaat tgttgaacag gttccctccc
      301 ggctacgcaa actttaccac accggtttta aatacgaatc ttcgcgccag ttctgttcaa
      361 aatttgtctt tgatatttat aaagaggcgc tatgtattcc ggtgggtgaa atagagacgt
      421 ttggagaatt gttaaatagc aatccgaatg caaaactcac tttctggaaa ttctggttcc
      481 tgggttctat tccgtgggag cgtaaaaccg ttacgccagc cagtttgtgg catcatccgg
      541 gtttggtgtt gattcatgcg gagggagtgg aaacgcctca gccggaactg accgaggcgg
      601 tataacttaa cgcagtcgcc ctctcgccag gttcagccgc gattcgctca tctgcatcgc
      661 attctgacta acgtggcagt gggtgatggc aatcgccaac gcatcagcgg catccgcctg
      721 tggattagcg ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc ttttttcggc
      781 gctaccaata cccaccaccg tttgcttcac ctgacgtgca gcatattcaa ataccggcaa
      841 ctcctgattc accgccgcca caatcgccac gccgcgcgcc tggcccagtt tcagggctga
      901 gtcagcgttc tttgccataa agacttgttc aatggcgaaa taatcaggct ggaactgggt
      961 gatgatttcc gtcacgcccg catagatgag tttcagacga gacggtaaat catccacttt
     1021 ggtgcgtatg catccgctac ccaggtagga cagttgccta cctacctggc ggatgacgcc
     1081 gtagccggtc acgcgcgaac ccggatcaat gccgagaata atagccatca cgcgtctccg
     1141 ttttgctgtt tagcaggcct catcagagag tcgctgcgac ctcatcagag atttcaccgt
     1201 tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga cgcatcagtt
     1261 tcggtgcggt ttctgcatcc atatcagctt tggtagacgg gatcatggaa acttccgcgc
     1321 tgtctgcttt cagacctgcc gcttccagag cgtcgcgcac tttacccatt tcttcccatg
     1381 cagtgtagac atcaatcgcg ccgtcatcat aggtcacaac gtcttcagca cctgcttcca
     1441 gtgctgcttc catgatggtg tcttcatcgc ctttctcgaa ggagatcacg ccttttttgc
     1501 tgaacagata agctacggaa ccatcagtac cgaggttacc gccacatttg ctgaatgcat
     1561 gacgcacttc agcaacggta cggttgcggt tgtcagacag acattcaatc atgattgccg
     1621 tgccgccagg accgtaacct tcgtagatga tggtttccat gtttgcatca tcatcaccgc
     1681 ccacaccacg tgcaattgcg cggttcagtg tgtcgcgggt catgttgtta gacagtgctt
     1741 tatcaattgc tgcacgcaga cgcgggttag cgtccggatc gccaccgccc agcttagccg
     1801 cggttaccag ctcacgaatg attttagtga agattttacc gcgcttagca tcctgcgcag
     1861 ctttacgatg tctggtgttg gcccatttac tatgacctgc cataaaaata tctccagata
     1921 gccctgcctg ttcaggcagc gttaattaca aactgttcaa tcgcctgccg gttgctccag
     1981 gacttagtga gcgccgccgc agcaggcgca tcaagccact tgtaagccag atgttcagtg
     2041 aaaacgatct gccgctcgtg cggaagcgca agacagaacc atgattccgt attacgcgtc
     2101 acgcccggcg catagcgatg acgtaaatgt gaaaaaattt caaactctac cgtgcgctga
     2161 cagtcaatta aggtcagttg ttcagcgaca acatcaatgg tgacctcttc ctttacttcg
     2221 cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc tgccggttac cgactgccag
     2281 aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg tgcgtagatg
     2341 accactaaga tcgaaacggg acgcttatac actttatcct tcacgctgcc tcttcgttga
     2401 ctgccttcgc tcatcccatt cacatagtta tctatgctca tgggagttca ctcagttgcc
     2461 gcctcgatgc aacgcgaatg atttcgtgta tttgagtcat atcagttatt ctcagccttc
     2521 ttcacaacct gaatgctcag ctcagccagt gcagtcgggt tagcaaagct cggtgcttca
     2581 gtcatcagac acgctgccgc cgtggttttc gggaaggcga taacgtcacg gatattgtcg
     2641 gtgccggtca gcagcatggt cagacggtca agaccgaatg ccagacctgc gtgcggcgga
     2701 gtaccgtatt tcagagcgtc gagcaggaag ccgaatttct cgcgctgttc ctcttcgttg
     2761 atacccagaa taccaaacac cgtctgctgc atatcaccat tatggatacg tactgaacca
     2821 ccgcccactt cgtaaccatt gatgaccata tcgtaagcgt tcgccaccgc attttccggt
     2881 gcagctttca gttctgcagc cgtcatatct ttcggtgagg tgaacggatg gtgcattgcc
     2941 gtcaggccgc cttcaccgtc gtcttcaaac atcgggaagt cgataaccca cagcggtgcc
     3001 catttgcttt cgtcggtcag accaaggtct ttacccactt tcaggcgcag tgcacccatc
     3061 gcgtcggcaa caattttctt gttgtcggca ccgaagaaaa tcatatcgcc atcttgcgcg
     3121 gcagtacgat ccaggatgtc ttcgatgatt tctgcattaa ggaacttcgc taccgggctg
     3181 ttgatacctt ccagaccttt cgcgcgttcg ttaactttga tgtaagccag acctttcgcg
     3241 ccgtagattt taacgaagtt accgtattcg tcgatctgct tacgggtcag cgatgcgccg
     3301 cccggaacgc gcagagccgc tacgcgacct ttcggatcgt tcgccggacc tgcaaataca
     3361 gcaaactcaa cagatttcag cagatcagca acgtcagtca gttccatcgg gttacgcaga
     3421 tccggtttat cagaaccata acggcgttct gcttccgcaa aggtcattac cgggaaatcg
     3481 cccagatcca cacccttcac ttccagccac agatgacgca ccagcgcttc catcacttca
     3541 cgcacttgcg gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa ttcaggctga
     3601 cggtcagcac gcaggtcttc gtcacggaag catttaacga tctgatagta acggtcaaaa
     3661 ccggacatca tcagcagctg tttgaacaac tgcggggatt gcggcagtgc gtagaattta
     3721 cctttgtgca cacgagaagg caccaggtag tcacgcgcgc cttccggcgt ggctttggtc
     3781 agcatcggag tttcgatgtc gaggaagccg tggtcatcca taaaacggcg caccaggctg
     3841 gtgattttag cgcgggtttt caggcgctga gccatttccg gacgacgcag gtcgaggtag
     3901 cggtatttca gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg cagaacatct
     3961 gcgcggttga tgatagtcag cgaggacgcc agcacttcga tttcgccggt cgccatatcg
     4021 cggttaatat ttttttcgtc acgcgcacgt acggtgcccg tgacctgaat gcagaactca
     4081 ttacgcagtt cagaggccag ctttaacgcg tccgcacgat ccggatcgaa aaatacctgc
     4141 acgatacctt cgcggtcgcg catatcgatg aagatcaggc taccaagatc acgacgacgg
     4201 ttgacccaac cacacagagt cacctgctgc cccacgtggg acaaacggag ctgtccacaa
     4261 tattctgtac gcatgagata tcccttaact tagctgccgg cggatgcccc ctgctgcgca
     4321 ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg cggctattat
     4381 actggaaatt ctgccgcacc gtaagagcct ggcccgcgct ggaacgcctc gttaccactt
     4441 tatatcgggc ctgaaatcag actctacgcc agtttgctat aaaggtgttg cccgaactca
     4501 taaaaattaa caaaatttgt cgttccgcca tcggctaatc gcattaaggt gtgaggcacg
     4561 attttgtttt ttcaggagtc attatgcttg aacttaatgc taaaaccact gcgctggtgg
     4621 tgatcgattt acaagaaggc atcttacctt ttgccggtgg tccacatact gccgctgagg
     4681 tagttaatcg cgccgggaag ctggcggcga aatttcgcgc cagcggtcag cccgtgtttc
     4741 tggtgcgcgt tggctggtct gccgattacg ctgaagcatt aaaacagccg gtcgatgcgc
     4801 cctcaccggc taaagtgttg cccgaaaatt ggtggcaaca tcctgctgca ttaggtgcaa
     4861 ccgacagcga tatcgaaatc atcaaacgtc aatggggtgc gttttacggt acggatctgg
     4921 agttgcaatt acgccgccga ggtatcgata caatagtgtt atgtgggatc tcgaccaata
     4981 tcggtgttga atccaccgcc cgcaatgcct gggaacttgg ctttaatctg gtgattgccg
     5041 aagacgcctg tagtgccgcc agcgccgagc agcacaataa cagtatcaac catatctacc
     5101 cgcgcatcgc ccgtgtgcgt agcgtggaag agatcctcaa cgcgttatga tttacatcgg
     5161 tctaccgcaa tggtcgcatc ctaaatgggt gcggttgggg atcaccagcc ttgaagagta
     5221 tgcccgccac tttaactgcg tggagggcaa caccacgctt tacgccctgc cgaaacccga
     5281 ggttgtcctg cgctggcgtg agcagaccac agatgacttc cgcttctgtt ttaagtttcc
     5341 ggcgaccatt tcgcatcagg cagcattacg gcattgcgat gatttagtga ctgaattttt
     5401 gacccgcatg tcaccgttgg ctccgcgcat tgggcaatac tggctgcaac tgcctgccac
     5461 attcggccca cgggagctgc ctgcgctttg gcattttctc gattctcttc ctggcgaatt
     5521 taattatggc gtggaagtcc gccatccaca gtttttcgcc aaaggagaag aggaacaaac
     5581 gcttaatcgc ggtttacatc agcgcggcgt taatcgggtg attttagaca gccgcccggt
     5641 tcatgcagca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt
     5701 tccggtacat gctgtactga cggcgacaaa tccgctgatc cgttttatcg gtagtgatga
     5761 tatgacgcaa aaccgggaat tatttcaggt ctggttacaa aaattagcgc agtggcatca
     5821 gaccactacg ccttatcttt ttttacatac gccagacatt gcccaggcac cggaactggt
     5881 acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat
     5941 tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca
     6001 ttttaaaggg agtttgtatg gtaagcgcgc tgtatgccgt tttaagtgcg ttgttattaa
     6061 tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggcgacg
     6121 gcggttttag cgaactgcaa agcgctattc gcattcatgg taacgcggtg gaatatattc
     6181 ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc
     6241 atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc
     6301 gtctgttccg ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg
     6361 tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa
     6421 tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct
     6481 gcccctatcg ccagactggg cgactggacc tttgatgaac gggtagctga agtcttcccg
     6541 gatatgatcc agcgttccgt acccggctat tccaatatta tttccatgat tggtatgtta
     6601 gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg
     6661 gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac
     6721 aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg
     6781 ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg
     6841 gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcatt actggataaa
     6901 atttatcaag gactgaaccc gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa
     6961 gatgccaaag ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt
     7021 tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat
     7081 tccgtggaaa cccataaagc acgcctgcat aaagccggtt ttgagcatag tgagctgtgg
     7141 ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg
     7201 actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc
     7261 tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggttgttt aagcagtggt
     7321 caaatgcggt ggagtttcta cctgaaatta aaccgtatcg tcttgattta ttgcatagcg
     7381 ttacggcaga aagcgaagag ccactgagcg cagggcaaat taaacgcatt gaaacgctga
     7441 tgcgcaacct gatgccgtgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata
     7501 ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg
     7561 gacgcaccat tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg
     7621 caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag
     7681 cggtgcgtaa actactgggt aatgatcagc gcgcgcattt gttaccgtta ggtattgaac
     7741 aacttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc
     7801 gttcaccgct ggagcatcta tggcagttga aagatcaact ggtcaatgaa ggtgaactgg
     7861 tgctggaaac gctggttatt gatggtgatg agaacacggt gctggtacca ggcgatcgtt
     7921 acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga
     7981 agaagtgtgg ttttgtagat atccgcattg cggatgtgag cgttaccacc acagaagagc
     8041 agcgacgcac cgaatggatg gttaccgaat cgctggccga tttcctcgac ccacatgatc
     8101 cgggtaaaac ggtggaaggt tatccggcac ctaaacgcgc ggtgctgatt gcgcgcaagc
     8161 cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc cttatccggc ctacaaaacc
     8221 ttgctaattc aatatattgc agggactatg taggcctgat aagcatagcg catcaggcag
     8281 ctttacgttt gcataacctc agcgcccgtt tccgggcgct attcacgtct tacgcctgaa
     8341 cagctggctg atcaaacgcc gttaacttcg gtgcattgcc cgtatatttt tcgatataca
     8401 ccaacgctga gttaccggca caggcatttg ccagctgcga gctggggata tccgccgtta
     8461 acacgttcgc actgccgttt ttacacaagc cattttccag atctggccat gcaccttcat
     8521 gcacgcatac cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac
     8581 cacgtttgtt ccagacacgc accagatcgc cattcgcaat accaaagcgt gcagcatctt
     8641 cggtgtgaat agtgattggt tcacgatctg caaccgcata ttttttacgc agttccgcat
     8701 agttaagctg actatgtaaa cggtgtgccg gatgtgcggt cagaagctgc aactgcttct
     8761 cgtcggcggt acccttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct
     8821 tatagccaaa tttttccagc gttttggagt aaatctcaat tttgccgctt ggcgtaccca
     8881 gcgcattttt caccggatcg gcgcggaaat caccataacg aacgtactgt tcgttcttct
     8941 cgctgcggcg catttcgatc agtttatttt gctgccagaa ggcattaaac atcggcatag
     9001 tcacgcgttg cgcacgggca cctttctgag cggcatcata gaaaaatttc agccacgcca
     9061 tttcatcttt accttcggta tagatctctt ttccgccagg tttcagtaat tccgcaagat
     9121 cggcaaacac atcaaaatcg tttcgcgctt caaattgcgg agcgacagcc tgcttcatcg
     9181 gcacaatatg ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg
     9241 tggtgatcgg taatacgata tctgcatgtt tagcagctgc ggtccagtag cattcagaaa
     9301 cgacgatcat ctccggtttc tgccatgctt taatcagacg attagtatcc tggtgatggg
     9361 taaagttacc gccacccgcc caccagatca ttttgatatt cggatacgtt tgctctttac
     9421 cgttatgctg atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa
     9481 tcgccgtcat tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca
     9541 tttccggcag cacgccgcca acacgtgtcg ggttgccacc attggagtaa tgataagaga
     9601 aaccaaaacc gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca
     9661 tccagtgttt ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg
     9721 tgcgattcgc cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa
     9781 tttccgccgc ccaggccgcg ctcttcggag tattatcgct cttacctgtc agatattctt
     9841 caaactgcgg atacccggtg gtgtattttt caaggaacac tttatcgtgt ttgccttgtg
     9901 tcatcagggt atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga
     9961 tccaggtggc attatcgcca aagaactcaa tggtttcgga gcggatagga tcaatggcaa
    10021 tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac
    10081 tgctccaggc aatttttaag gtattcagcg ggttcatccc ccacagcacc acaacctggc
    10141 tgttttccag aatcagcggc caggaggtct gctgttcata cacctctaca gaaccgacca
    10201 catgcggcat gatcacctgt gctgccccgg ttgaataatc gccgctatgc ccggaataac
    10261 cgcccgcgag gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag
    10321 agcgccagcc gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta cgaatacgat
    10381 cgtgttgctc atgaatcagt tttaatgctt gttcccagct cacctgtaca taggtatctt
    10441 cgcctcgacc tttcgccggt tgcagtggat tatcgagata gctttttctc accatcggat
    10501 gctgaatacg cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gagttcggta
    10561 tggttttcgc cagcgcgcct gtcgaagaaa caatcttgcc gtccttcact tctacgttca
    10621 tcgctcccca gcgtcccgcg gtgaggattt taccgccctt ctcttctgcc catgcgggta
    10681 gcggtgctgc cgatgtcacc accagcgctc cagcggcaat accgctgtgt ttaataaatt
    10741 cacgtcttgt taatgtcata acttcctccc tgatcaacga ggatcactgt ttctcggtaa
    10801 tatctttggc gttgtactgg aaataccgcg ttaaaatgtc cagttcgttt tcgctcatgc
    10861 tggttcgtgc ccccattcct ttggcaatgg acggccacgc attgacggtg taatggtcgg
    10921 cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc
    10981 atagcggttt acggtccgct aatgcgggat cggtaagcgc accctgtaaa gacgcctgac
    11041 gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgtgagc gccttgatac
    11101 cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt
    11161 cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa
    11221 cgacggtgag atcggtacca ggattcacgg tagccagctc gcctatgcga gaagttttga
    11281 aaggataaat atgtgcgcca ttagtcactg aagtagcggt ctgactttcc agctcatgcg
    11341 ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta tgacaatcga
    11401 tacaggtttc gccgcccttt tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg
    11461 aggcaatatc catggcatca aaagaatggc aactacggca cgttgcagag tcagttgctt
    11521 ttaattcttt ccatactgtt tcggccattt cctggcgatg agtttcgaac ttatcgtcac
    11581 tgtctatttt gccgctaaca aattcatgat aaatatcttt agatgctttt aatttagcaa
    11641 ataaataatc catccctgac tttggaatat ggcaatcggc acattccgca cgtatccctt
    11701 tctagttcga aaagtggaca gttccctgat attcctcaaa aggtttactc atcgagtggc
    11761 aggaaaggca aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc agcaataacc
    11821 cgccgccacc aaccacaacc gctatcagca aaaacaataa cccaatgcgt tttttccctc
    11881 gcatagattc ctctaaataa attccagaac accgaaatca ttgtataatt aaatacacaa
    11941 cgaattaacc gcttgcataa ctcggcacaa cactgcctga aataatcgat aaagaatatg
    12001 atttattac
//
LOCUS       DAPGWD010000126        11906 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPGWD010000126 DAPGWD010000000
VERSION     DAPGWD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.127"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            243..1631
                     /gene="narU"
                     /locus_tag="RHX88_003373"
     CDS             243..1631
                     /gene="narU"
                     /locus_tag="RHX88_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDV2232031.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1713..5453
                     /gene="narZ"
                     /locus_tag="RHX88_003374"
     CDS             1713..5453
                     /gene="narZ"
                     /locus_tag="RHX88_003374"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDV2232032.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIESIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLILGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5450..6994
                     /gene="narH"
                     /locus_tag="RHX88_003375"
     CDS             5450..6994
                     /gene="narH"
                     /locus_tag="RHX88_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV2232033.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6994..7689
                     /gene="narW"
                     /locus_tag="RHX88_003376"
     CDS             6994..7689
                     /gene="narW"
                     /locus_tag="RHX88_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV2232034.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7686..8366
                     /gene="narI"
                     /locus_tag="RHX88_003377"
     CDS             7686..8366
                     /gene="narI"
                     /locus_tag="RHX88_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV2232035.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8446..9339
                     /locus_tag="RHX88_003378"
     CDS             8446..9339
                     /locus_tag="RHX88_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDV2232036.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWVIEL"
     gene            complement(9435..10280)
                     /gene="nhoA"
                     /locus_tag="RHX88_003379"
     CDS             complement(9435..10280)
                     /gene="nhoA"
                     /locus_tag="RHX88_003379"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDV2232037.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPRKIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10453..11022
                     /locus_tag="RHX88_003380"
     CDS             10453..11022
                     /locus_tag="RHX88_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDV2232038.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11026..11253)
                     /gene="pptA"
                     /locus_tag="RHX88_003381"
     CDS             complement(11026..11253)
                     /gene="pptA"
                     /locus_tag="RHX88_003381"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDV2232039.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11307..11906)
                     /locus_tag="RHX88_003382"
                     /pseudo
     CDS             complement(11307..11906)
                     /locus_tag="RHX88_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2817 a         3083 c         3100 g         2906 t
ORIGIN      
        1 gaagggttga aacatgacat acgttcaaag attaaccccc gtttgaaggt tagcttgtta
       61 gttacattta gtaacacata tctggctact taatccccat tctgcccccg cggttacgct
      121 ttcttatcta ttctttcaat cagcacttca cccaattcat ccttaattct aataactggc
      181 tattgcgcct tacctcctgt tttgtctttt ttcattacca atgtgtgcat gtgaggaaca
      241 atatggcact gcaaaatgag aaaaatagtc gttatctttt acgcgactgg aaaccagaaa
      301 atccggcctt ctgggaaaat aaaggaaaac atattgctcg aagaaacctc tggatatcag
      361 tcagttgtct acttcttgcc ttctgtgtct ggatgctatt tagcgcagtt accgttaatc
      421 tcaataaaat cggttttaat tttactaccg atcaactctt tttattaacc gcattaccct
      481 ccgtttctgg cgcattattg cgtgttccct actcctttat ggtgcctata ttcggtggac
      541 gccgatggac ggtttttagt actgcaatcc tgattattcc ttgcgtctgg ctcggaattg
      601 ccgtgcaaaa tccgaatact ccttttggga tatttatcgt tatcgctttg ctatgcggtt
      661 ttgcaggtgc aaactttgct tcgagcatgg gcaatatcag tttcttcttt ccaaaagcca
      721 aacaagggag cgctcttggg attaatggcg gattaggaaa cttaggtgta agtgtaatgc
      781 agctggttgc accgctggtc atttttgtac ctgtatttgc ctttctcggc gtcaatggcg
      841 taccgcaggc cgacggttcg gtgatgtcgc tggcgaatgc cgcatggatt tgggtaccgc
      901 tactggcgat tgccacgatc gccgcatggt cagggatgaa tgatatcgcc agttcacgcg
      961 cctcaattgc cgaccagctc cctgtcttac aacgcctgca tctctggctg ctgagcctgc
     1021 tttaccttgc caccttcggt tcgtttatcg gtttttctgc gggttttgcc atgctggcaa
     1081 aaacccagtt cccggatgtg aatattctgc gcctggcgtt ctttggccca tttatcggtg
     1141 ccatcgcgcg gtcggttggt ggtgctattt ccgataagtt cggcggcgtg cgggtgacgt
     1201 tgatcaactt tatttttatg gcgattttca gtgccctgct gttccttacc ttaccgggca
     1261 caggctccgg taatttcatc gccttttacg ccgtatttat ggggctgttt ctgactgcgg
     1321 gtctgggaag tggttctact ttccagatga tcgccgtcat ctttcgccag ataaccattt
     1381 atcgggtaaa gatgaaaggc ggtagtgatg agcaagctca gaaagaagcc gtcaccgaaa
     1441 cggcggcggc tctgggcttt atctcagcca ttggcgcagt gggcggcttt tttattccgc
     1501 aggcgtttgg catgtcgctc aatatgaccg gctctccggt cggcgcgatg aaagtgtttt
     1561 taatcttcta catcgtttgt gtgctgctga cctggctggt ttatggtcgg cggaagttca
     1621 gccaaaaata agagatcgat gcctgatgta caaccgtgtc gcatccgaca aggcatcaga
     1681 tcccaattca ttcctggagc aggagtcatg tcatgagtaa acttttggat cgctttcgct
     1741 acttcaaaca aaagggcgaa acctttgccg atggtcacgg acaggtgatg catagcaacc
     1801 gcgactggga ggacagctat cgccagcgtt ggcagttcga caaaatcgtg cgttccaccc
     1861 acggtgttaa ctgtacaggc tcctgtagct ggaaaatcta cgttaaaaat ggtctggtga
     1921 cctgggaaat ccaacagacc gactacccgc gcactcgccc tgacctgccc aatcatgaac
     1981 ctcgcggctg cccgcgtggc gcaagttact cctggtatct ttacagcgct aaccgcctga
     2041 aatacccgct cattcgtaaa cgactgattg aactgtggcg cgaagccctc aagcaacaca
     2101 gcgatccggt actggcgtgg gcatcgatta tgaacgatcc gcaaaagtgc ctgagctaca
     2161 aacaagtgcg tgggcgcggc gggtttatcc gctccaactg gcaggaacta aaccagctga
     2221 ttgccgccgc taacgtctgg accatcaaaa cctacggccc ggatcgcgtt gccggtttct
     2281 cgccgatccc ggcgatgtcg atggtttctt acgccgccgg aacgcgttat ctgtcactgc
     2341 ttggcggcac ctgtttaagc ttctacgact ggtattgcga cctgccgccc gcctcgccga
     2401 tgacctgggg cgagcaaacc gacgtaccgg aatctgccga ctggtataac tccagctaca
     2461 tcatcgcctg ggggtctaac gtaccgcaga cacgtacgcc ggacgcccac ttctttaccg
     2521 aagtacgcta caaaggcact aaaaccatcg ccattacccc tgactactct gaagtggcca
     2581 aattgtgcga ccagtggctg gcaccgaaac aaggcactga tagcgccctg gcgatggcaa
     2641 tgggccatgt gattttaaaa gagtttcatc tcgataatcc cagcgactac tttatcaact
     2701 actgccgccg ctacagcgac atgccgatgc tggtaatgct ggagcctcgc gacgatggta
     2761 gctacgttcc cgggcgcatg atccgcgcat ctgacctggt ggatggactg ggcgaaagca
     2821 acaatccgca gtggaaaacc gtagcagtta ataccgcagg tgaattggta gtgccgaacg
     2881 gttcgattgg tttccgctgg ggagaaaaag gcaaatggaa tctggaatcc attgccgccg
     2941 gtacggaaac cgaattgtcg ttaaccctgc tcggtcaaca tgacgctgtt gcaggcgtgg
     3001 ccttccccta ctttggcggc attgaaaatc cgcattttcg cagcgtaaaa cacaatccgg
     3061 tgctggtgcg ccaattgccc gttaaaaacc tgacgttagt cgatggcaac acctgtccgg
     3121 tggtcagcgt ttatgatttg gtactggcga attacggcct cgatcgcggg ctggaagatg
     3181 aaaacagtgc gaaagattac gctgaaatca aaccgtacac cccagcctgg ggtgagcaaa
     3241 ttaccggcgt gccgcgccag tatattgaat ccatcgctcg tgaatttgcc gatactgccc
     3301 ataaaacgca tgggcgctca atgattatcc tcggcgctgg cgtcaaccac tggtatcaca
     3361 tggatatgaa ctaccgtggg atgatcaata tgctgatctt ctgcggttgt gtcgggcaaa
     3421 gcggtggcgg ctgggcacac tatgtcggcc aggaaaaact gcgcccacaa accggctggt
     3481 tgccactggc ctttgcgctc gactggaacc gaccaccgcg ccaaatgaac agcacctcgt
     3541 ttttctacaa tcattccagc caatggcgct atgaaaaagt ctctgctcag gagttacttt
     3601 caccgctcgc cgatgccagt aagtacagcg gtcatctgat tgatttcaac gttcgcgccg
     3661 aacgtatggg ctggctacct tctgcgccgc agctggggcg taacccgctc gggattaaag
     3721 ctgaagccga caaggccgga ttatccccca cagaatttac cgcccaggcg ctgaaatcgg
     3781 gcgatttacg tatggcctgc gaacaaccag atagcggcag caatcatccg cgtaatttgt
     3841 ttgtctggcg ctctaacctg cttggctcct ccggcaaagg ccacgagtat atgcagaagt
     3901 atctgctggg gaccgaaagc gggattcagg gcgaggaact cggtgccagc gacgggatca
     3961 aaccggaaga agtcgagtgg caaactgcgg cgattgaagg caagctcgac ctgctggtga
     4021 cgctcgactt ccgcatgtcc agtacctgcc tgttctccga tatcgttctg cccaccgcca
     4081 cctggtacga aaaagacgat atgaacacct cggatatgca tccgtttatt catccgcttt
     4141 ctgcggcggt cgatccggcg tgggaatcac gcagcgactg ggaaatctac aaaggtatcg
     4201 ccaaagcatt ttcgcaagtg tgcgtgggtc atcttggcaa agaaaccgac gtggtattac
     4261 aacccctact gcatgactct ccggcagagc tctcacagcc gtgtgaagtg ctcgactggc
     4321 gcaaaggcga atgcgatctg attctaggca aaaccgcgcc gaatattgtg gcggtggagc
     4381 gcgactaccc tgctacgtat gaacgcttta cctcgctcgg gccattgatg gacaaacttg
     4441 gcaatggcgg taaagggatt tcgtggaata cgcaggatga aatcgatttc ctcggcaaac
     4501 tcaattacac caagcgtgat ggcccggcac aggggcgtcc gctgattgac accgccattg
     4561 acgcttcaga agtgattctg gcactggcac cggaaaccaa cggtcatgtt gcagtcaaag
     4621 cgtggcaggc gctgggcgag atcaccgggc gcgaacatac ccatctggcg ctgcacaaag
     4681 aggacgagaa gattcgcttt cgcgatattc aggcgcagcc gcgtaaaatt atctccagcc
     4741 ccacctggtc cggtctggaa agcgatcacg tatcctataa cgcgggatac accaacgttc
     4801 atgagttaat tccgtggcgc acgctgtcgg gacgccagca gctctatcag gatcatccgt
     4861 ggatgcgtgc ttttggtgaa agcctggtgg cttatcgccc gcctatcgac acccgtagcg
     4921 tcagtgagat gcgccagata ccgccaaacg gcttcccgga aaaagcactt aacttcctga
     4981 cgccgcacca gaaatggggc attcactcaa cctacagtga aaacctgcta atgctgacgc
     5041 tctctcgcgg tggaccgatt gtctggatca gcgaaacaga tgcccgagaa ctgaccattg
     5101 tcgataacga ctgggtggaa gtattcaacg ccaacggcgc gctgacggcc cgcgcggtgg
     5161 tcagccaacg tgtaccgccg ggcatgacca tgatgtatca cgcccaggaa cgcattatga
     5221 atattcctgg ttcggaagta actggcatgc gcggcggcat tcataactcg gtcacccgcg
     5281 tttgcccgaa accaacgcat atgattggcg gttacgcgca gctggcctgg ggctttaact
     5341 actatggcac cgtcggatcg aaccgcgatg agttcatcat gatccgcaag atgaagaacg
     5401 ttaactggct ggatgatgaa ggtcgcgatc aggtacagga ggcgaaaaaa tgaaaatccg
     5461 ttcacaagtc ggcatggtgc ttaacctcga caaatgtatc ggctgccata cctgttcggt
     5521 gacctgtaaa aacgtctgga ccgggcgcga aggcatggag tacgcatggt ttaacaacgt
     5581 cgaaaccaaa ccgggcattg gttatccgaa aaactgggaa gatcaggaag agtggcaagg
     5641 cggctgggtc cgcgatgtga atggcaagat ccgtccgcgt ctgggcagca agatgggcgt
     5701 aataaccaaa atcttcgcca acccggtggt gccgcagatt gatgattact acgaaccttt
     5761 caccttcgac tacgaacatt tgcatagcgc accggaaggc aaacatatcc ctactgctcg
     5821 cccgcgttca ctgattgacg gcaaacggat ggacaaagtg atctggggcc caaactggga
     5881 agaactgctg ggcggcgagt ttgaaaaacg tgcccgcgac cgcaacttcg aggccatgca
     5941 aaaggagatg tacgggcagt ttgaaaacac cttcatgatg tacctgccgc gcctgtgcga
     6001 acactgcctc aatcccagtt gcgtggcgac ctgcccaagc ggcgctatct acaaacgcga
     6061 agaagacggc attgtgctga ttgatcagga taaatgccgt ggctggcgtt tgtgcattag
     6121 cggttgtccg tacaaaaaaa tctacttcaa ctggaaaagc ggcaagtcag aaaaatgtat
     6181 cttctgttat ccgcgaattg agtcaggcca acccaccgtg tgctcagaaa cctgcgtggg
     6241 tcgcatccgg tatctgggcg tgctgcttta cgacgccgac cgcattgagg aagcggcgag
     6301 caccgagcgc gaagttgacc tctatgaacg tcagtgcgaa gtgttcctcg atccacacga
     6361 tccctcagtg atcgaggaag ccctgaaaca aggtattcca caaaacgtga ttgaagctgc
     6421 ccagcgttcg cctgtctaca aaatggcgat ggactggaaa ctggcgctac cgctgcaccc
     6481 tgaatatcgc accctgccaa tggtctggta cgttcctccg ctgtcgccga ttcagtccta
     6541 cgcagatgcg ggcggtttgc cgaaaagcga aggcgtgctg cccgccatcg aaagcctgcg
     6601 tattccggtg caatatctcg ccaatatgtt gagtgccggc gataccggtc cggtactgcg
     6661 ggcgctgaaa cggatgatgg cgatgcgcca ctatatgcgt tcacaaaccg tggaaggcgt
     6721 tactgatact cgtgccatcg acgaagtagg cctgagcgtc gcccaggtcg aagaaatgta
     6781 tcgctatctc gccattgcca actatgaaga tcgttttgtt atcccgacca gccatcggga
     6841 aatggcgggc gatgccttcg cagaacgcaa cggctgcggt tttacctttg gcgacggttg
     6901 ccacggttcg gacagtaaat tcaacctgtt caacagtagc cgtatcgatg ccatcaacat
     6961 caccgaagtg cgcgacaaag cggagggcga ataatgcaga tcctcaaagt gatcggcctg
     7021 ttgatggagt atccggatga gctgttgtgg gaatgcaagg aggacgcgct ggcgttgatc
     7081 cgccgcgacg cgccgatgct tacggatttc acccacaacc tgcttaacgc gccgctgctg
     7141 gataaacagg ccgaatggtg cgaagtgttt gaccgcgggc gcaccacgtc gctgctgctg
     7201 ttcgaacatg ttcatgccga gtcccgcgat cgcggccagg caatggtgga cctgctggcg
     7261 gagtatgaaa aggtcggcct gcagctggat tgtcgggaac tgcccgatta tctaccgctg
     7321 tatctggagt atttaagcgt actgcctgac gatcaggcga aagaaggatt gctcaatgtt
     7381 gcgccgatcc tcgccctgct tggcggtcgc ttaaaacaac gcgaggcacc gtggtacgcg
     7441 ttgtttgatg ctctgctgca actggcggga agtatacttt caagtgacag tgtcacaaaa
     7501 caagtaaaca gcgaagagcg cgatgacacc cgccaggcgc ttgatgcagt gtgggaagag
     7561 gaacaggtga agtttattga agataacgcc acggcgtgtg acagctcgcc gcttaatcaa
     7621 tatcagcgac gctttagcca ggatgtcgcg ccgcagtatg tcgacatcag tgcgggaggt
     7681 gggaaatgat tcagtatctg aacgtctttt tttacgatat ctacccgtac atttgtgcga
     7741 cggtgttttt cctcggcagc tggctgcgct acgactacgg gcagtacacc tggcgcgcct
     7801 cctcaagtca aatgctcgat aaacgcggga tggtgatatg gtcgaatctg ttccatatcg
     7861 gcattttggg gattttcttc gggcacttgt tcggcatgtt aacgccgcac tggatgtacg
     7921 cgtggttttt gccagtggca gcgaaacaac tcatggcgat ggtgctcggc ggtatttgcg
     7981 gtgtgttgac gttgattggc ggcgcaggac tgctgtggcg caggctgaca aatcagcgcg
     8041 tgcgtgcaac ttccactacg ccggatatta tcatcatgag cattttgctg atccagtgtc
     8101 tgctggggct aagtaccata ccgttttcag cacagtatcc tgatggtagc gaaatgatga
     8161 agctggtggg ttgggcgcaa agcattgtca ctttccgtgg tggatcgtca gaaatgctta
     8221 acggcgtagc gttcgttttc cgcctgcatc tggtattggg aatgaccatc ttcctgctct
     8281 tcccgttcac cagattggtg catgtgtgga gcgcgccgtt tgagtacttt actcgtcggt
     8341 atcagattgt gcgttcgcgg cgataatttt caccctggcc ggatagccct tcgctctccg
     8401 gccacttgca ggctatgata gttctccagc cacaggagaa cgttaatgaa accgcaggtg
     8461 taccacgtcg atgcttttac ctcacaaccg tttcgcggca attctgccgg tgttgttttc
     8521 cccgccgata atcttagcga agcgcaaatg cagcttatcg cccgcgaatt aggtcattcg
     8581 gaaactgctt ttctgctgca cagcgacgac agcgatgtgc gcatccgcta ctttacgcca
     8641 acagttgaag tgccgatttg cggtcacgcg acagtagctg cgcactatgt acgtgcgaag
     8701 gtgttaggtt taggaaattg cacggtctgg caaacatcgc tggcaggaaa acatcgcgtg
     8761 actatcgaaa agcacaacga tgattatcgt atttcgctgg aacaaggtac gccgggcttt
     8821 gaaccgccgc tggagggtga aacacgtgag gcgattatca acgcgcttca tctcactgag
     8881 gacgatattc tgccaggctt gccaattcag gtggcaacaa caggacactc aaaagtgatg
     8941 atcccgttga aaccagaagt ggatatcgac gccctttcgc ctgatcttgc tgcgctgagc
     9001 gctatcagca aacagattgg ctgcaatggt ttcttcccgt tccagatccg cccaggcaaa
     9061 aacgaaaccg atggtcgcat gttctcgcct gcgattggta ttgtggaaga tccggtgact
     9121 ggcaatgcca atggcccgat gggtgcatgg ttggtacatc acaacgtatt gccccacgat
     9181 ggcaaggtgc tgcgtgttaa aggccatcag gggcgcgcat tggggcgtga cggcgtgatt
     9241 gaagtgacgg tgacaattcg tgataaccaa ccggaaaaag tcaccatttc aggcacagcg
     9301 gtgattttgt ttcatgccga atgggtaatt gaactctgaa atcgttgcct gatgcgcttc
     9361 gcctgtcagg tctacaaccg ggctaatgta tgccggataa ggcgattacg ccgcatccgg
     9421 caacctgaca taaattattt tcccgcctcc ggatgcgtat caaacgccgc catcaccagc
     9481 gctaactcat ccacagtaaa gccatgtttc gcatcatcca cgcccagacc aaactgttcc
     9541 tgcatcacgg catataacga caccacatcc ggtagatttc gctgctccac cgcgtgccca
     9601 ttttcataat gggtaaaatg aaaattggtc agcgttagct tgccaccgtc cggcaaatgg
     9661 cggcacatca gcaaatgatg gcgaaaatgc gactgcggcc agtgcgccga ccagaaattg
     9721 cccatcacat aatcgctttg ttgctgctcg cagagatcaa aacggtacat cgactgccaa
     9781 tgctgatgat gattaaactg caacacccaa tcatcaccct cctgcaacaa ccgatactct
     9841 ccgtgtggcg tggtctgcac gagatcggaa actaaacgaa tcggcgcggt tagcgtctgc
     9901 ccaccgaaac cgacatcagc aatccatttt tcctcttcca gttccaccaa cagcaaacga
     9961 tgggtgcgcg gcggtaatgc tggcggattt gataacacta cgcgccctaa caagctgcga
    10021 acgttaaacc ccagctcgcg taacacccgc tcaaacacgc cattctgctc aaaacagtaa
    10081 ccgccacgac gggctatcac cagtttctct tccagcgatt gatcatcaag ctgtattttc
    10141 ctcggcagca aaacgtcaag gttttcaaac ggaatggtgc aattgtgttt caggtgcaat
    10201 gcacgaagcg tatcaatatt gaccgcagca gctcccgacc agttaatacg ggcaaaatag
    10261 tgattcagaa tgggcgtcat caggcgtttc ccttagtggt tttctcaact tataacctgg
    10321 aatagacgcc gcaccggcta ttttgtgcga tcgcggacat gcatcacgtt tgcaaatcgc
    10381 caatcccgtt aatttcgtta cttattgcgt tacggactac gcttaaaaaa accttcttcg
    10441 cgaggcgata ccatgagccg attcatcccc atagaattac accatgccag ccgtctatta
    10501 aatcatggcc caactgtcat gatcaccagt ttcgatgagc aatcccagcg gcgtaacatt
    10561 atggctgcag cctggtcaat gccggtggag tttgaaccgc cacgcgtggc gattgtggta
    10621 gataaatcga catggaccag ggagttgatt gaacgtaacg gtaagtttgg catcgttatc
    10681 ccgggcgttg cagcaactaa ctggacgtgg gcagtgggaa gcgtgtcggg tcgtgaagaa
    10741 gataaattta attgctatgg cattccggtg gtgaaaggtc cggtgcttgg tttgccactt
    10801 gttgaagaga aatgcctggc gtggatggag tgccgcctgc tacctgcgac ttctgcgcaa
    10861 caaaaatacg acacgctgtt tggcgaggtg gtatcagcag cggcagacgc acgggtattt
    10921 gtcgaaggcc gctggcagtt tgatgatgat aagctcaata cgttgcatca tttaggtgct
    10981 ggcacgtttg ttactagcgg caagcgtgtt acggcaggtt aagcattaca tgctgtaacc
    11041 gggtttcttt atcaaagcct ccatttgggg cgcgatttcg gcatcccaga tagcttgcca
    11101 agattctggt tgaatctgct gtagagcaat gcttatcgaa ctgtctttac tgttcagatg
    11161 gcgaataata acgtcggtaa tatctgcagc aagtgctgct ttttgttgtt cgtccagttc
    11221 acgcggaaag catttaatgt cgatgtgcgg catgattgtg ctccttttga aagatgtcag
    11281 taaaattaac acacattgtg aattttcagt cctttccatt caaacgtgaa atcaataagt
    11341 tcatcaggga catcgcaaac aatatgaata cggatttctt ctctgccgtg actcttttca
    11401 caaattgcgt aactgtcatg ctctggatta tttaattctt tcagcggaaa tttttcctca
    11461 aaggctttat ttagccaccc ctggtttcct ttcaccgcga ataaataatc acctccctgt
    11521 ttttgtatct tctctgctat atctttctgg caacccatcg catcagttgt gatgattttt
    11581 cctttaatat ccagcatgtt aagaagttct gggatagctg taatctcatt agatttatca
    11641 tccgtcttga tctgtccgat gaccagactg tgcattgttg agaacgcact aatgacatga
    11701 atcgctcccc tgcggcgact cttatcataa gaatgccgga gcgtttttcc atcaattgca
    11761 atgacgtctt tatcatctga agaatgacaa tcacgcatcc agttaataaa gcactcatga
    11821 aattttgtag ggctgataca ggatacaact ctggcaatgg tatcgtgaac aggaatacca
    11881 ttttcaaaat caccatattg cttcaa
//
LOCUS       DAPGWD010000127        11793 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPGWD010000127 DAPGWD010000000
VERSION     DAPGWD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.128"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            88..2244
                     /locus_tag="RHX88_003383"
     CDS             88..2244
                     /locus_tag="RHX88_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HDV2232040.1"
                     /translation="MHNEDSEDDNLATFTLSGGRNAEYSITATQRQNFSKTTGALARW
                     ILPGEVITVGGVEITGGYFYLGQRMNPVGHNSGGYYDDSSEASLIDDTLKIYPTSYIY
                     EDSSLGYWPSYSSLSPEARGAYLSWLTSDRCDASCPIGYVFIYLYGLERRALVDSRDS
                     NISDDEFYALFKEICRLRSVFNENRSFRNYSSQLLEAMSILRPNENLAAALSDDSEFS
                     NSMQFRLILAKTVDAGAPVPADLALTWVTNHTEYSLRTPARRCAKEFAALFKRRYTLK
                     YGEGMVVKPNKARLRLDYTPASPSLRGIRLPVPDLPDPSALKGPVQKLMTLADICTDE
                     LDAYSRYLGRKGTSANDTVAILLLPSEIVNESADKILSAFKRWADDAILSKGGVVSVA
                     DFWSHMNATCPAKINKKEADLMQAFAQKMGYCLAPDPYYHHVKADVDGVLVLFPAGES
                     GRFSPSPEFITAVLTLRLGSVVALIDNSFDQAEQKVLENAINNNVSFSDDEKRSLHAY
                     LTWQLHTPANMTGMKSRIELLGAAEKSAVGKVIVSVACSDGRITPAEIKQLEKIYTSL
                     GLDPSSVSSNIHQHSVTETALVSSVPANQSTAGFTLDANVLARHESATDDVRKLLNSI
                     FTEEEPEQPESAPASLTGEGGLDSAHSQLYRRLLEKEQWSRKEATELCSNWNLMLGGA
                     LEVINDWSYAVVDAPVLEDADDHIWVDLEIAKELEG"
     gene            2248..3564
                     /locus_tag="RHX88_003384"
     CDS             2248..3564
                     /locus_tag="RHX88_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV2232041.1"
                     /translation="MSVTRIRVKERDAIIQSLKSGVTPRIGIQHIQVGRVNEITALHQ
                     DIERIADGGASFRLIIGEYGSGKTFFLSVVRSIALEKKLVTVSADLSPDRRIHSSGGQ
                     ARNLYSELMKNMSTRNKPDGNALLSVVERFVTEARKEADANGSQVSSVIHKRLAALSD
                     MVGGYDFAKVIDAFWRGHEQDNETLKSNAIRWLRGEYTTKTDARNDLDVRTIISDASF
                     YDSLKLMSLFVRQAGYAGLLVSLDEMVNLFKLNNTQARTANYEQILRILNDCLQGSAE
                     NIGFILGGTPEFLFDPRKGLYSYEALQSRLAENRFAQKAGVIDYSSPTLHLASLTPEE
                     LYILLKNLRHVYAGGDPANYLVPDEALTGFLHHCSKTIGDAYFRTPRNTIKGFLDMLA
                     VLEQNSTMNWQALIEGVAIEEDKPSDMGDAIVEESDDDDGLANFRL"
     gene            3561..5759
                     /locus_tag="RHX88_003385"
     CDS             3561..5759
                     /locus_tag="RHX88_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV2232042.1"
                     /translation="MSSAYNSLDPRVRKWIYKQGWSSLRPLQESSIPAILARDRDVLI
                     SAGTAAGKTEAFFLPACSAIADLTGGFGIIYISPLKALINDQHRRLESLGEALEMQVT
                     PWHGDVPQSKKKKARTSPSGILLITPESLESLLINSLGWLKQAFSSVAYIVIDEFHAF
                     IGSERGIQLLSLLNRIDHVLGRQLNPVPRVALSATLGELEKVPELLRPDKRLPCETVT
                     DSNSTATLQVQVKGYLERVPEKGEELQSSAEQLVCADIFRLCRGDSHLVFANSRKRTE
                     SIAAILSDMCEANVVPNEFFPHHGSLARELREALEARLQKGNLPTTAVCTMTLELGID
                     IGKVQSVIQVTPPHSVSSLRQRMGRSGRRDSPSVLRMLITEPELTATSSIVDHLRLQL
                     VQAMAMIRLMISKRWFEPADIRQKHYSTLLHQILAITAQWGGVRADQLWSQLCQTGPF
                     RNVDINDFKSLLKHMGTCGLLSQLASGEIVVGAEGEKLTNHYTFYAVFNTPEEFRIVT
                     GNRTLGTVPVDSPLLPEQHIIFGGRRWKVTEIEVEKKVIYVEATKGGQPPLFSGSGMS
                     VHDVVRQEMLAIYRENDYRIAVGNKRVDYADAAARSLFAEGSDNFQRYNLHNDCFIAS
                     GQNCYVIPWMGDKIVNTITSLLIRCGFKASSFAGVIEVEDSGVASVQRVLKKMLLSGL
                     PSEFELAAVVPDKYLDKYDEYLPETLLAKGYGAQAYDIEGTCTWLQKHLQ"
     gene            6241..7257
                     /locus_tag="RHX88_003386"
     CDS             6241..7257
                     /locus_tag="RHX88_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232043.1"
                     /translation="MSSVRTDKFIRYGFAVVAIVAILITTITTPNPTPYQYIVYRIVL
                     ALAAGCIGAVLPGFLEVKFNGWLRASGAVAIFAIVFFISPDLLQQNPESASKPVPPTV
                     IDAYAVNSSNRDRYLELFRTGQLGQRDKLRIGCVEWSESSCVAAGNFLRLLSEAGWEI
                     DSDAVFRMQPSVPVEGVSIVSRGDDLVGLPKVPPHMGRWSRSDISYEILTAAFKLMGV
                     PVYFSSDPTLQPGTTGVYFGPKPKLDAPVMQKDENVRRELLRYVGLGMTIQRACSQQP
                     VELCGGEIFSWETSVSSFLRQQRFNSDAVNRWLALPQVNAASATADMKIKLDLLMSFV
                     LGIE"
     gene            7901..10256
                     /locus_tag="RHX88_003387"
                     /pseudo
     CDS             7901..10256
                     /locus_tag="RHX88_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
     gene            10433..11572
                     /locus_tag="RHX88_003388"
                     /pseudo
     CDS             10433..11572
                     /locus_tag="RHX88_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(11569..11724)
                     /locus_tag="RHX88_003389"
     CDS             complement(11569..11724)
                     /locus_tag="RHX88_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232044.1"
                     /translation="MTKGNISERDFDILIKLSGIHSEKAILALKKYYVDGVIRKIDPT
                     HVMWTRP"
BASE COUNT         3059 a         2645 c         2958 g         3131 t
ORIGIN      
        1 actaagtccg cgggctctta tacttccgct cctgtgaaag caccacctaa agaatggctt
       61 gctaataaca aaaaaaatca gtctgtaatg cacaacgagg acagcgaaga tgacaatctg
      121 gctacgttta cactaagtgg cgggcggaat gccgaataca gtataacagc cactcaacgc
      181 cagaatttct caaaaactac cggtgctttg gctcgctgga ttttaccggg ggaggtgatc
      241 acagtcggtg gggtagagat taccggcggc tacttctatc ttggacagcg aatgaaccct
      301 gtcgggcata actcaggcgg ttattatgat gatagtagcg aggcatcgct aattgacgac
      361 acgctcaaga tataccccac atcttacatc tacgaagata gttcattagg gtattggccc
      421 agttattcat cactttcacc tgaagcacgt ggcgcgtacc tgagttggct taccagcgac
      481 agatgtgacg cttcttgccc aattggttac gtttttattt atctgtatgg tctggaaaga
      541 cgggcgcttg ttgacagcag ggacagcaat atatctgacg atgagttcta tgctcttttc
      601 aaagagatct gccgtctcag atctgttttc aatgaaaaca gatcattccg gaactactcg
      661 tcccagttac ttgaagcgat gtccattctt cgtccgaacg aaaatcttgc agcagctctc
      721 agcgatgatt cagagttcag caatagcatg cagttcaggt taatcctggc aaagactgtt
      781 gatgcaggag ccccggttcc agcggatctg gctctgacgt gggtgacaaa ccatactgaa
      841 tactcattac gaacccccgc ccgccgctgt gcgaaagaat ttgctgcact atttaaacgg
      901 cgttacaccc ttaaatacgg tgaagggatg gtagtgaagc cgaataaagc gcgtctgcgt
      961 ttagattaca cgcctgcaag tcccagtttg agaggtattc ggcttcctgt tcctgacttg
     1021 cctgatccaa gtgcattgaa aggccctgtt cagaagctta tgactcttgc tgatatatgc
     1081 acagatgaac tggatgctta cagtcgttac cttggtagaa aaggcacttc agcaaatgat
     1141 accgttgcaa ttttgttgct gccttcagaa atcgttaacg aaagcgcaga taaaatatta
     1201 agtgcattca aacgctgggc tgatgacgcg atcctcagta aggggggagt agtttcagtt
     1261 gctgacttct ggtcacacat gaatgctacc tgtccggcaa agatcaacaa aaaagaggcc
     1321 gacttgatgc aggcctttgc acagaaaatg gggtactgcc tggctcctga cccctattac
     1381 caccacgtaa aagcagatgt cgatggtgtt cttgttttat tccctgctgg tgaaagtggc
     1441 cgcttctctc cttctcctga atttatcaca gctgtattga cgctccgatt aggttcggta
     1501 gtcgccttga ttgacaattc tttcgatcaa gccgaacaga aagtgcttga gaatgccatc
     1561 aacaacaatg tcagcttcag cgatgatgaa aagcgctctc tgcacgcata tttaacgtgg
     1621 cagcttcata ccccggcgaa tatgacggga atgaaaagcc ggattgaatt actcggtgca
     1681 gcggaaaaat cagctgtggg taaggttatc gttagtgtcg cttgctctga cggcagaatt
     1741 acgcctgccg aaatcaaaca gcttgagaag atttatacaa gcctggggtt ggatccctca
     1801 tccgtatcaa gcaatatcca tcagcattcc gtaactgaaa ctgctctcgt ttcatctgta
     1861 ccagctaatc agtcgacggc agggtttacg ctggatgcca atgtacttgc ccgccacgaa
     1921 tctgccactg atgatgttcg taaattactg aactcgattt tcactgaaga agaaccagaa
     1981 caaccagaaa gcgctccagc ctcattaacc ggggaagggg ggcttgactc cgctcatagc
     2041 caactttatc gcaggttgct ggagaaagaa caatggtcca gaaaagaggc gacagagttg
     2101 tgtagtaact ggaacctgat gctcggcggt gcgcttgagg tgattaacga ttggtcctat
     2161 gcggtggttg atgcaccagt gctggaagat gctgatgatc acatttgggt tgacctggaa
     2221 atagccaaag aattagaggg ttagtgaatg tctgtaacac gtataagagt gaaagagcgc
     2281 gacgctatta ttcagtcact taagtcggga gtaacgccaa ggataggtat tcagcatatc
     2341 caggttggcc gcgtgaatga gataaccgcc ctccatcagg atattgaacg gattgctgat
     2401 ggcggggcaa gcttcagact tatcattggt gagtatggtt ccggcaaaac gttcttttta
     2461 agtgttgtac ggtctattgc gttagagaaa aagctggtca cggtcagtgc agacctttct
     2521 cccgacagac gtattcactc atcgggaggt caggctcgta atctctactc agagcttatg
     2581 aaaaatatgt ccaccagaaa caaaccagat ggcaatgcat tacttagcgt tgtggaaagg
     2641 tttgttactg aggcaaggaa ggaagctgat gcgaacggct cccaggtctc atccgttatt
     2701 cataaacgcc ttgctgcttt gtcagatatg gtgggtggat atgactttgc gaaggtcatt
     2761 gacgcattct ggcgcgggca tgagcaggat aacgaaacct taaaatccaa tgctattcgc
     2821 tggttgcggg gcgagtacac cacaaaaact gatgcccgta atgatcttga cgtccgaacg
     2881 attatctcag acgcctcgtt ctatgactct ttgaagttga tgagcctttt tgtccgtcag
     2941 gcaggctatg ccggactttt agttagcctc gacgagatgg tgaacctctt caagttgaat
     3001 aatacgcaag cgagaacggc caactacgaa caaattttgc ggatcctgaa tgactgcctg
     3061 caaggctccg ccgaaaatat tggttttatc ctcgggggga cgccagagtt tcttttcgat
     3121 ccacgtaagg ggctctacag ctatgaagcg ctccagtcgc gtctggcaga aaatcgattc
     3181 gcacagaaag ctggcgtgat tgactactcg tctcccacgc tacatcttgc cagcctcact
     3241 cctgaggaac tgtatatttt actgaaaaat cttcgtcatg tttatgctgg tggagatccc
     3301 gcaaattatc tggtgccaga tgaggctctg accgggtttc tgcatcactg cagcaaaacc
     3361 atcggagatg cttactttcg aactccccgc aataccataa aaggttttct ggatatgctg
     3421 gctgtgctgg aacaaaacag tacgatgaac tggcaagctc tcattgaggg ggtggcgatt
     3481 gaagaagaca agcccagcga tatgggggat gccattgtgg aggaaagtga tgacgatgat
     3541 ggcctggcga atttcagact atgagcagtg cctacaatag tctcgatccc cgtgtccgta
     3601 agtggattta taagcagggc tggtcatcat taaggccctt gcaggagagc tctattccgg
     3661 caattttagc ccgtgatcgt gatgtactaa ttagtgcagg tacagctgcg ggtaaaacag
     3721 aggctttctt tcttcctgcc tgttcagcga ttgcagatct cactggtgga tttggcatta
     3781 tctatatcag tccgttgaag gcattgatta acgatcagca ccgtcgtctt gagagcctgg
     3841 gggaagccct ggaaatgcaa gtgacgccct ggcacgggga tgtaccacaa agcaaaaaga
     3901 agaaagcacg gacaagcccg tcaggtatct tacttatcac acctgaatca cttgagtctt
     3961 tgctcataaa ctcattgggt tggctcaaac aggcgttttc ttcagtggca tacattgtta
     4021 ttgatgagtt ccacgctttt attggctcag agcgtggtat acaactgctt tcgctgctta
     4081 atagaattga ccatgtgctg ggacgccagt taaatccagt tccccgtgtc gcgttgagtg
     4141 ccactctggg agagctggaa aaagtgccag aattattgcg tccggataag agattacctt
     4201 gtgaaacggt aacagacagt aatagcaccg ctacactaca agttcaggtt aaaggttatc
     4261 ttgagcgggt gcccgaaaaa ggggaagagc tccagagctc tgctgaacag ctcgtctgcg
     4321 cggatatttt caggctctgt cgtggcgatt cccacctggt ctttgctaac agtcgaaagc
     4381 gtactgaaag catagcggca atactgagcg acatgtgtga agcaaatgtc gttcctaatg
     4441 aattctttcc tcatcatggc tcccttgcca gggagttacg tgaggcgctt gaagcccgac
     4501 tgcagaaagg gaatttgccc acaaccgctg tttgtacaat gacgctggag ctgggtattg
     4561 atattggtaa ggtgcagtct gtcatacaag tgacacctcc gcattctgtt tccagtctgc
     4621 gtcaacgcat gggacgttca gggcggcgcg attccccatc ggtgctgaga atgttaatta
     4681 ctgaacctga acttaccgca accagcagca ttgtggatca tttacggctg cagttggttc
     4741 aggcgatggc aatgatcagg ttgatgatct ctaagcgctg gttcgagcct gcagatatcc
     4801 ggcagaaaca ttactccaca ctactacacc agatcctggc tattactgcg caatggggag
     4861 gtgttcgtgc cgatcaactc tggtcgcaac tttgccagac aggtccgttc cggaatgtgg
     4921 atataaatga ctttaaaagt ctgcttaaac atatggggac atgtggcctg ctcagtcaac
     4981 tagccagcgg tgaaatagtt gtcggggctg agggggagaa gctgactaat cactacacgt
     5041 tttatgccgt attcaataca cctgaggagt ttcgcattgt aacgggaaac cgaactcttg
     5101 gcacagtgcc tgtagactcc ccacttttgc cggagcagca catcatcttc ggcggacggc
     5161 gctggaaagt gacagagatc gaggttgaaa aaaaggttat ctatgttgag gcgactaaag
     5221 gcggtcagcc accactattc agtggtagcg ggatgtctgt tcatgatgtt gttcgccagg
     5281 agatgctcgc tatctacagg gaaaatgatt accgtatcgc ggtaggcaac aagagagtcg
     5341 attatgctga tgccgcagcc agaagcctct ttgcggaagg aagtgacaac ttccaacgct
     5401 acaatctaca caatgactgt tttattgcca gtggtcagaa ctgctacgtg atcccgtgga
     5461 tgggggataa aattgtcaat acaattacgt ccctgcttat acgttgtggc tttaaagcca
     5521 gttcatttgc aggtgtgata gaggtagagg actcaggcgt tgcctctgtc cagcgtgtac
     5581 ttaagaaaat gctattgtcg gggttacctt cagagtttga gcttgctgca gttgttcctg
     5641 acaagtatct ggataaatat gacgagtatt taccagagac tttgcttgct aaagggtatg
     5701 gggcacaggc gtatgatata gaaggaacgt gcacctggct gcagaaacac cttcagtaga
     5761 ctggatatgt agtcagggta taaataactt atttataccc tgattttttt acttcccggt
     5821 ggacttactt ggctttagtc ccggagtgtt gccaggagta tctttattat ctcgtcggcg
     5881 ttgatctggt tttcctgtcg atttagcaat tggttttgga ccaggtttaa gacccatgat
     5941 agttatcctt ttgagatgta agagggaagc cctcaacatt ggtatattgg atactgagaa
     6001 atttttcaat gggagtgata aacggccgaa tgattaactc gatattgtga tcaacgtctg
     6061 ttttttgcac actgtagact gactcttgga gcagaaggtt gctctgagcg aggaatggac
     6121 agttacactc atcaccaacg ccaatagcct cattacgcgt ataaagctgt attaatcatt
     6181 ggggaacagt ttaatgcggg attttcgact tatggacttg attcaggtgt caaaattaaa
     6241 atgagcagtg taaggacaga caagttcatt aggtatggtt ttgcggttgt ggcgatagtt
     6301 gccatactca ttactactat cacaaccccc aatcccacac cttatcaata tatagtttac
     6361 cgaatagttc tggctctggc tgccggatgt attggtgcag ttctgcctgg tttccttgaa
     6421 gtaaaattta atgggtggtt aagagcatct ggcgcagtgg cgatcttcgc tatagtgttt
     6481 ttcataagcc cagatttgct tcaacaaaat cctgaatctg caagtaaacc tgtaccgcct
     6541 actgtgattg atgcatatgc cgttaatagt tccaatcgcg acagatacct cgaattattt
     6601 cgaacaggac agctcggtca gcgggacaag ctgcgcatcg gttgtgttga gtggagtgaa
     6661 tcatcgtgcg tggctgctgg taattttttg cgattgttaa gcgaagctgg ctgggaaatc
     6721 gattctgacg ctgtattcag aatgcaacca tccgtgccag ttgagggggt atcgattgta
     6781 agccgtggcg atgatctggt cggtttgcca aaagtaccac cccacatggg ccgttggagt
     6841 cgatcagaca ttagttatga aattttgaca gcggcattta aattgatggg agttccggtt
     6901 tatttttcca gtgacccgac attacaacca ggtactaccg gagtgtactt tggaccgaaa
     6961 ccgaaactgg atgcaccagt catgcaaaaa gatgagaatg tgcgacgtga gcttctccgc
     7021 tacgtcggtt tgggaatgac tattcagcga gcctgctctc agcagcccgt cgaactttgt
     7081 gggggtgaaa tcttttcctg ggaaacgtcg gtatcttcgt tcctaaggca gcagagattc
     7141 aactccgatg ccgtaaacag atggcttgcc ttgccgcagg ttaatgcggc ttcggcaact
     7201 gcagacatga aaataaagct agatttgtta atgtcatttg ttctgggcat cgagtaacta
     7261 tacctcttgc gaggatgatt gagcgtctaa tggaccagct tagccgttag cgctatttgt
     7321 ttacgtaagc ctgtggaatc caactagtgc ttctacgatg cgttgtttgg tgaaaaaaaa
     7381 gtgttgaagc actggtttta gacaactaaa gccacgattt gctcatgcgt cgaaaatgta
     7441 gtcgtctact gtgtagcacg gaggcgatat tcgtacgctt acacttgccg ataggccact
     7501 ggggagtgtt cttggcacta agctgccagg cccgaaggtc tgctatgagc gaggggctga
     7561 cattcctatt ggcattcggt gtgagttatc gggggcggtc atgcggcata cttccgatac
     7621 aggcgtttcc agctctgcct gtttcagggt tatattaatc tgctttagaa ttaatcgtgg
     7681 ctttagcatc tgcacatcgt gctggagaaa cctgagtgaa ctaaatcata ttccctttct
     7741 tcagctaatc aattgatagt tatgcggaaa aagttacttt gcttgtcaat aatccatttc
     7801 acatccgtta atccaatgct atgcgtcttc gcattaatct ggaaaggtag taagtgaaat
     7861 aaaaacttag ccaatgacta atatctgagg atactttgtc atgaacagta tgcaatcaaa
     7921 aacgggctct ccactcgttc gaactgaagc cgagctagaa agccggctta cttcagcact
     7981 aaacatagca tttcctaaca ttcctcgcga agatcttatc gaacagcgcc attttaccgt
     8041 tcggctaggc catggaactt acaaaataga tagcgccgca cactggaaaa aatacgggcg
     8101 agcggatgtt ttgatctttc atcgggagag accacttgcg gttattgaac tgaaacgtga
     8161 agacctgaca ctgacccatg atgattatga acaggctcaa agctacgcta accagttaac
     8221 accacgtccg cctctcgttg tagttacgaa cggtaaagat acgcgcgttt atgactcaag
     8281 taatggacaa cagtggtcgg gtggacagga tgcctcggca gcggtaaaca agatgctcgc
     8341 caattctgca aagttggcgg cggctgacat gcgctgggct attgaagcct tgatggggcg
     8401 agaaactaat gcttgggtgc cagctgtgcg agaagaaaca gcccggctgc tgattgatat
     8461 aactgatcag cctgggcatt cagaacatcc atttgccaac aacctacttt ttcctcgcaa
     8521 aatcacgtct cttgtaattg aatctgctgt gatgggtaca gctttcacta tcattgcggg
     8581 tgatgcacaa agcggaaaaa gcagttgcct ccgtgaaatt tcgctaaaaa cggaaagtag
     8641 cgatcttttg gctgtcctga tgttacgagg ttccggacca ggactatttc aggctctggc
     8701 caatcttttt gcagctgaat ttgaatggaa tttaacctca aatgatgcac gtaactggtt
     8761 aagacgaatg tctaactgca ctgaaggtcc atcgcttatg cttgctatcg acgacgttga
     8821 accaggctcc caaatggcta cttatcttga ggaacttgcg ggtatacgtt ttggcaacag
     8881 gctggtggtt gttctgacga cctatcatgc caacgcgtta ctgaaaaatc caaacggacg
     8941 aactccgtct gcaattggta gccggtcgaa ggtatttaaa acatcgccta tgtccctaga
     9001 tgaatttaag ctggcgcaac agattctctc ggatcagcgg atagtttttc aacagggagc
     9061 agaatacgcc gatgactacc gctcgccttg ggttcttcgc actatttatg atgacattgt
     9121 gcgcaatcat caataccaga aaacggatct tatcgcctat cttcctccat cacccggtat
     9181 ggaacttata gatgctgcgc aaaagagcta tgagagtcag tatgatcttc ttcgttatta
     9241 tcgggttctt gcacgctgcg cactagctga cacaaactcc cactcagtag aattaatgtt
     9301 tgctaaagcg aatggatttg tcgtgcggta tgacgcactt tctgacgaag ctcgtggtgt
     9361 agtgaatgag ttaaaacata tgggggcagt tcgtattttt cgcctttcag gatgggaaga
     9421 tgttgtggtg ccgacagttc ccgcagctta ccttctggaa ctgtcggatg ctgtttgtga
     9481 tgaactggtt ttacgggcag aacaagaccc tcaagacgcc ggggcttggc gaacggctag
     9541 acgccactta tcttggagat atgatcggtg ctcaagccat acgccgtatg gcagctaaag
     9601 agagatattt cagctttgga attattcagg gccttctcag cattgagcca tataaagaac
     9661 ctataaaaaa cggattgttt actttagcca tgccagacaa ccaacaagtc aatctaaaga
     9721 ttgaggatgg attagcgtgg atttctaaac acggggatga tgcaaagagt gttctggtga
     9781 atttagagga tcaaatccca aaggtcattt ctaaatcaac cagttggatg atactcgggc
     9841 aactagcgaa actgccgtca gcagaggtgg gtgacgatga tcagcgtata gacgcctata
     9901 ttttactgag cattgggcgc tgcccatttc ctctaattcg cacgaatata gaaggattgc
     9961 cttactttga gcataatttt ggcgatcagg gtgatgttct ttgcctagaa aaagcgtcta
    10021 ttgaagttgc tactcaggcg atggctgatt tattttcagc cccatggctc tatgcggacc
    10081 agtgggtgga tacagctatc gcgaccggtt ctatacattt actgcatcgt ttgtttgctg
    10141 ctttaaacac agtgatattg cgtagaatac ccgtgcaatc cgactgggct aatgaagcac
    10201 ttaatcaacg tgtctcacca gcccttaagg aagccatacg atcattatct tcgtgactaa
    10261 gcaatgggat tttaatacat aacgagcatc tgtccacgca agttaacaat tgccttggct
    10321 cgatattttg gtttctagtg catatatgct gatcttctct tcgttgatct gatcttaccc
    10381 accaatagtg gacacaggac taagtgagta aactctcaac cagaggtgac tcatgacaaa
    10441 accagtatca atcagcaaga agccccgtga acaacattcg cctgaatttc gtaacgaagc
    10501 cctgaaactc gctgaacgca tcggtgtggc cgccgcagcc cgtgaactca gcctttatga
    10561 atctcagctt tatgcctggc gcagtaaaca gcagcaacaa atgagttcgt cagagcgcga
    10621 aagcgaactg gccgctgaaa atgtccgcct taaacgacaa ctggcggagc aggctgagga
    10681 actggccatc caccaaaagg ccgcgacata cttcgcgaat cgcctgaaat gaagtatgtc
    10741 ttcatcgaaa atcatcgggc agagttctgc atcaaagcga tgtgtcgtgt acttcgggtt
    10801 gcccgcagcg gctggtatgt ctggctcagg cgtcgtcacc agatgagcct tcgccaacag
    10861 tttcggctca cctgcgatgc cgctgtccat aaggcattct ttgaggcaaa gcagcgatac
    10921 ggtgctcccc gccttgctga cgaactgccg gagttcaata ttaaaaccat tgccgccagc
    10981 ctgcgtcgtc aggggctgcg ggctaaagtt cagcccggtc agctaccgtg cacatggcct
    11041 gcccgtattg gagaatctgc tggagcagga cttcagcgcc agaggcccga accagaagtg
    11101 ggcgggtgac atcacgtact tgcgtaccga tgagggctgg ttgtatctcg cagtagtcat
    11161 cgacctgtgg tcacgcgccg tcattggctg gtcgatgtca ccgcgaatga cagcacaact
    11221 ggcctgtgat gcactacaaa tggcgttgtg gcggagaaga cgcccggaaa gcgtcattgt
    11281 tcatacggac cgtggtggtc aatactgttc aggggattat caggcgctgc tgaagcgaca
    11341 caacctgcgt ggcagtatga gtgcgaaagg ctactgttat gacaatgcct gtgtggaaag
    11401 cttctttcat tcgctgaaag tggaatgtat ccacggggaa cgctttagca gtcgggaaat
    11461 aatgcgggca acggtgttta attatatcga gtgtgattac aatcgctggc gtcgtcacag
    11521 tgcctgtggc ggtctcagcc cggaacaatt tgaaaaccat aatctcgctt agggccgtgt
    11581 ccacattacg tgggtaggat caattttcct gatgacccca tcgacatagt attttttcaa
    11641 agcaagaatt gccttctcgc tatgtattcc tgatagcttg atcaggatat caaagtctct
    11701 ttcagatata ttcccctttg tcatgtgtca ctccatgaat caaatttcat agtaatcaat
    11761 ctttctttga tcgacttata gataataaga tca
//
LOCUS       DAPGWD010000128        11333 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPGWD010000128 DAPGWD010000000
VERSION     DAPGWD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.129"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            190..1761
                     /gene="garD"
                     /locus_tag="RHX88_003390"
     CDS             190..1761
                     /gene="garD"
                     /locus_tag="RHX88_003390"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDV2232045.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1910..2245
                     /gene="prlF"
                     /locus_tag="RHX88_003391"
     CDS             1910..2245
                     /gene="prlF"
                     /locus_tag="RHX88_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDV2232046.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2245..2709
                     /gene="yhaV"
                     /locus_tag="RHX88_003392"
     CDS             2245..2709
                     /gene="yhaV"
                     /locus_tag="RHX88_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDV2232047.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2764..3573)
                     /gene="agaR"
                     /locus_tag="RHX88_003393"
     CDS             complement(2764..3573)
                     /gene="agaR"
                     /locus_tag="RHX88_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDV2232048.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3822..5102
                     /gene="kbaZ"
                     /locus_tag="RHX88_003394"
     CDS             3822..5102
                     /gene="kbaZ"
                     /locus_tag="RHX88_003394"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDV2232049.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5125..5598
                     /gene="agaV"
                     /locus_tag="RHX88_003395"
     CDS             5125..5598
                     /gene="agaV"
                     /locus_tag="RHX88_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV2232050.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5609..6388
                     /locus_tag="RHX88_003396"
     CDS             5609..6388
                     /locus_tag="RHX88_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDV2232051.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6378..7256
                     /locus_tag="RHX88_003397"
     CDS             6378..7256
                     /locus_tag="RHX88_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDV2232052.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHANKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7274..7708
                     /locus_tag="RHX88_003398"
     CDS             7274..7708
                     /locus_tag="RHX88_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV2232053.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7705..8838
                     /gene="nagA"
                     /locus_tag="RHX88_003399"
     CDS             7705..8838
                     /gene="nagA"
                     /locus_tag="RHX88_003399"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV2232054.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9189..10343
                     /locus_tag="RHX88_003400"
     CDS             9189..10343
                     /locus_tag="RHX88_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDV2232055.1"
                     /translation="MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLHKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10356..11216
                     /gene="kbaY"
                     /locus_tag="RHX88_003401"
     CDS             10356..11216
                     /gene="kbaY"
                     /locus_tag="RHX88_003401"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDV2232056.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2724 a         2928 c         2997 g         2684 t
ORIGIN      
        1 aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc gggcggcact gcaatccctg
       61 aaatgattga cattgatcac atttctgcgt ttaaactcct gacattctta tttcacccaa
      121 tgaagtcatt tatttttaaa tgagaccagg tcctcatttt aataacccct ggctggagaa
      181 tattgcacaa tggccaacat cgaaatcaga caagaaacgc caactgcgtt ttatataaaa
      241 gttcacgaca cagataatgt ggcaattatt gttaatgata atggcctgaa agcaggaacg
      301 cgttttccgg atgggctgga attaattgaa catattcccc aggggcataa agtcgcattg
      361 ctggacattc cggctaatgg tgaaattatt cgttatggcg aagtgattgg ttacgccgtg
      421 cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg ttgtactacc ggaagcgccg
      481 ccgttacaca cgctgccact ggcaaccaaa gtcccggccc ccttaccgcc gctggaagga
      541 tacacctttg agggctatcg caatgccgat ggcagcgtgg gcaccaaaaa cctgctcggc
      601 atcaccacca gcgtccactg tgtggcaggc gtggtggact atgtagtaaa aatcattgaa
      661 cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg tggggctgaa tcatttgtac
      721 ggctgtggcg tggcgattaa cgcaccggcg gcagttgtgc ctattcgtac catccacaat
      781 atttcgctga accctaactt tggcggcgaa gtaatggtga ttggtctggg ttgtgaaaag
      841 ttgcagcctg agcgcctgct ggtcggcacg gatgatgtgc aagctattcc agtagaaagc
      901 gccagcattg tcagtttgca ggatgaaaag catgtcggtt ttcagtccat ggtcgaggat
      961 attttgcagg tcgccgaacg ccatctacaa aaactgaatc aacgtcagcg agaaacctgt
     1021 ccggcttcag aactggttgt cggcatgcag tgcggtggca gcgatgcctt ttctggcgta
     1081 acggcaaacc cggcggttgg ctatgcgtct gatctactgg tgcgctgcgg cgcaacggtg
     1141 atgttctcag aagtcacgga agtgcgtgac gcgatccatc tgctgacacc acgcgcagtg
     1201 aacgaagagg tcggcaaacg gctgctggag gagatggagt ggtacgataa ctatctcaat
     1261 atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca acaagaaagg cggtctggca
     1321 aacgtggtag agaaggcact cggctccatt gctaaatcgg gtaaaagcgc aattgttgaa
     1381 gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa tttacgccgc gacgccagcc
     1441 agcgattttg tctgtggcac gcaacaggtg gcttcgggta tcaccgtgca agtgtttacg
     1501 accggtcgtg gtacgccgta cggcctgatg gcggtacccg tcattaaaat ggcgacccgc
     1561 accgagctgg cgaaccgctg gtttgattta atggatatta acgcgggcac tatcgccacc
     1621 ggcgaagaaa ccattgaaga ggtgggctgg aagttgttcc actttattct cgacgtcgcc
     1681 agcgggaaga agaaaacctt ctcggatcaa tgggggctgc ataaccagct ggcagtgttt
     1741 aacccggcac cggtgacctg atttcttttg gaacgtccct cgcaaaacat ggccttagtg
     1801 ccatgttttt attgtttaaa gcccccacgt ccattaataa tgcatttgca ttaccttagt
     1861 tcaagcttat aatttgagca gaaaacaggc tgtaaaagga cagtgaatca tgcccgctaa
     1921 tgctcgctct cacgctgtac tgaccaccga atcaaaggtc acgatacgcg gacaaacaac
     1981 tatccccgcg ccagtgcgtg aggccttaaa actgaagcca ggccaggaca gcattcatta
     2041 cgaaattctg cctggtgggc aagtctttat gtgccgactg ggagatgaac aggaggatca
     2101 tactatgaat gcatttttgc gttttctgga tgcagatatc cagaacaacc cgcaaaaaac
     2161 tcgtccattc aacattcaac aaggaaagaa acttgtcgct ggcatggacg tcaacattga
     2221 tgatgagatt ggcgacgacg aataatggat tttccacaaa gggttaatgg ttgggcgcta
     2281 tatgctcatc cctgttttca ggaaacctac gacgctttag ttgccgaagt cgagacatta
     2341 aagggaaaag atcctgaaaa ttatcagaga aaagccgcca caaagttatt ggcggtagtc
     2401 cataaagtga ttgaggagca tatcacggtc aatccatcat caccggcatt ccgtcatggc
     2461 aagtcgttag gctctgggaa aaataaagac tggtcacggg taaaatttgg tgctggtcgt
     2521 tatcgtctct tctttcgtta tagtgaaaaa gagaaagtca tcattctggg atggatgaac
     2581 gatgaaaaca ctctgcgcac ctacggtaaa aaaacagatg cctataccgt attcagcaaa
     2641 atgttaaaaa gaggacatcc tcctgccgac tgggaaagcc tcacccaaga gacagaagaa
     2701 aaccattgat ggtgttcaca atgcccgccg aacgtattcg tgttgaccag cgggcaccct
     2761 ttctcactcc ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc
     2821 cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg
     2881 taaactggaa cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc
     2941 gcacatccga cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag
     3001 atcgatcgca tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc
     3061 agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag
     3121 ctcaacgcct tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat
     3181 tacgtcagtg tgcttacgca tcagacgagc aatctcaaaa gtggtagtcc cggaatcgag
     3241 gatcacccga tgaccaggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat
     3301 cgcggtgttc agcgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc
     3361 gcagatcaac gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaacgccag
     3421 atcattgcgg atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac
     3481 actcccttgc tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgccagtcac
     3541 tcgcttctca cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt
     3601 tcatttcgtt ttgcctatta acgcctttct attaagcaaa tgcaagccca ccttgcccat
     3661 tgacgcaagc tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcagatcag
     3721 aaaactatta tctttcgttt tatttttatc tcaccatgac gcagtatcaa ctgaaacaaa
     3781 acgaaagatt aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa
     3841 tggtgagaca gcacaaagcg ggcaaaacaa atggaattta tgccgtttgt tccgcacatc
     3901 cgctggtgct ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg
     3961 aagcaacctc caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt
     4021 ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgttgattc
     4081 tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct gccggccgct caggcaatgg
     4141 ccaatgccga tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg
     4201 attgcagcat gtcctgtcag gacgatccga ttcccttaac tgatgacatc gtggctgaac
     4261 gcgccgcccg tctggcgaaa gtggcggaag aaacctgtct tgaacacttt ggcgaagccg
     4321 atctggagta tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa
     4381 gcgagctggc ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg
     4441 cctttgaaaa gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac
     4501 ccggcgtcga attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct
     4561 taagccagat ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc
     4621 aaacgccgca atcgctgcgc cagctggtga ttgaccactt tgccattctg aaagttggcc
     4681 cagcgctgac cttcgccctg cgtgaagctc tgttctctct ggcggcgatt gaagaagaac
     4741 tggtgccagc gaaagcctgt tctggtctgc gtcaggtgct ggaagacgtg atgctcgacc
     4801 gcccggaata ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt ctggcgcgtg
     4861 gttatagcta ctcggatcgc gtgcgctatt actggccgga cagccagatt gatgacgctt
     4921 tcgctcatct ggtacgtaat ctggcggatt caccaattcc gctgccgctg atcagccagt
     4981 atctgccgct gcagtacgtg aaagttcgct ccggcgagct gcagccaacg ccacgggaac
     5041 tcattatcaa ccatattcag gacatcctgg cgcagtacca cacagcctgt gaaggccaat
     5101 aagcaaaaca aagaggaaca cgctatgcca aatattgttt taagccggat tgatgaacgc
     5161 ttgattcacg gtcaggtcgg cgttcaatgg gtcggatttg cgggggcaaa tctggtgctg
     5221 gtagccaacg atgaggttgc cgaagatccg gtacaacaaa acctgatgga aatggtactg
     5281 gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag ttatcgacaa cattcatcgc
     5341 gccgccgatc gacagaaaat cctgctggtt tgtaaaacac ccgccgattt cctgacgctg
     5401 gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca atatgcacta cgccaatggc
     5461 aaacaacaaa tcgccaaaac ggtttctgtg gatgcgggcg atatcgcagc atttaacgac
     5521 ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc tgctgtggac
     5581 ctctttaaat tactttgagg gattcatcat ggaaatcagc ctgttgcagg catttgcgtt
     5641 gggcattatc gcctttatcg ctggcctgga tatgtttaac ggcttaaccc atatgcaccg
     5701 cccggtggtc ctcggcccgt tggtcgggct ggtacttggc gatctgcata ccggaatttt
     5761 aaccggcggt acgctggaac tggtgtggat ggggctggcc ccgctagcgg gcgcacagcc
     5821 gcctaacgtg attatcggta ctatcgtcgg cacggcgttt gccattacta ctggcgtgaa
     5881 acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc gcagtacaga tggggattac
     5941 cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc gaccggatgg cggagaacgc
     6001 cgatactcgc ggcattgaac gcgtgaacta tctggcactg ctggcactcg gcactttcta
     6061 ttttctctgc gctttcctgc ctatctactt cggcgcggaa catgcaaaaa ccatcattga
     6121 tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg ggcggcatca tgccagcaat
     6181 cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc tacatcccct acttcatcct
     6241 gggctttgtt gccgcagcct ggctcaagtt accggtgctg gctattgctg ccgctgcgct
     6301 ggcgatggcg ctgatcgacc tgctgcgtaa atctcctgaa ccgactcaac ctgcggcaca
     6361 gaaagaggaa tttgaagatg gcatctaatc aaaccaccct gccgaacgtc agtgaaaacg
     6421 aagaaacatt gctgactggc gtcaatgaaa acgtgtatga agatcagagc attggcgcgg
     6481 agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc catgctgtta caagcgtctt
     6541 ttaactacga acgtatgcag gcttctggct ggttgtacgg tctgctgcct gctctgaaaa
     6601 agatccacgc taataaacgc gacctggcgc gcgccatgaa ggggcatatg ggtttcttca
     6661 atacccatcc gtttctggtg acatttgtta tcggcattat ccttgcgatg gagcgttcta
     6721 agcaggacgt taacagtatt cagagcacca aaattgccgt cggtgcgccg ctcggcggga
     6781 ttggtgatgc gatgttctgg ctgacgctac tgccgatttg tggcgggata ggcgcaagcc
     6841 ttgcgctaca aggctctatt cttggcgcag tcgtatttat tgtgctgttc aacgtggtac
     6901 acctggggct gcgttttggt ctggcgcatt atgcttaccg catgggcgtg gcggcgattc
     6961 cactcattaa agcgaatacc aaaaaagtcg gccatgctgc gtctatcgtt gggatgacgg
     7021 taatcggcgc gctggtggca acctatgttc gtttaagcac cacgctggaa atcacagcgg
     7081 gcgacgcagt ggttaagtta cagactgatg ttattgacaa actgatgcct gccttcttac
     7141 cgctggtcta caccttgacc atgttctggc tggtacgccg cggctggagt ccgctgcgtc
     7201 tgattgcagt taccgtggtt ctcggcatcg tcggtaaatt ctgccatttc ctttaaatac
     7261 aatgaggtat tagatgttaa gtattatttt gacagggcat ggcgggtttg ccagcggcat
     7321 ggaaaaagca atgaagcaaa ttctcggtga gcaatcgcag tttatcgcca tcgattttcc
     7381 ggaaacctca agcaccgcgc tgcttacatc gcagcttgaa gaggccatcg cccaactgga
     7441 ttgtgaagac ggcattgttt ttctgacgga tttgctgggc ggcacgccgt ttcgcgtagc
     7501 ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatc accggcacca atttgcaact
     7561 gctactggag atggtgctgg agcgcgaagg gttaagcggt gaagaattcc gtgtacaggc
     7621 gctggagtgt ggacaccgag ggctgaccag tctggtggac gagttaggcc gctgccatga
     7681 agaatgtccg gtcgaggaag gaatatgaca cacgttctgc gcgccagaag gctgctgact
     7741 gaagagggat ggctcgatga ccatcagttg cgtattgctg acggtgtcat cgcagcaatc
     7801 gaaccgattc cagtgggcgt gactgaacgc gatgcggaac tgctctgccc cgcttacatc
     7861 gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc ggatgtactc
     7921 gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca gttggttgcc gactaccgta
     7981 accgcgccgc ttaacagcat tcatgcggcg ctgaaacgta ttgctcaacg ttgccaacgc
     8041 ggcggacctg gtgcgcaagt gctggggagt tatctcgaag gaccgtactt cacgccgcag
     8101 aataaaggcg cgcatccgcc ggaattgttt cgcgagcttg aaattgccga gctggatcaa
     8161 ttgattgccg tttctcagca caccttacgc gtggtagcgc tggcaccgga aaaagagggg
     8221 gcattgcagg ccatccgcca tcttaaacag caaaatgtac gagtgatgct ggggcatagc
     8281 gcggcgacct ggcaacaaac tcgcgccgcg tttgatgctg gtgccgacgg cctggtgcat
     8341 tgctataacg ggatgacagg tttacatcac cgcgaaccgg gaatggttgg cgcgggatta
     8401 acggacaagc gcgcctggct ggaactgata gccgatggtc atcatgtgca tccggcggcg
     8461 atgtcgctgt gttgttgctg tgcaaaagag agaatcgtgc tgatcaccga cgcgatgcag
     8521 gcagccggga tgccggatgg tcgctatacg ttatgtggcg aagaagtgca gatgcacggt
     8581 ggcgttgtcc gtaccgcgtc cggtgggctg gcgggcagta cgctgtctgt tgatgcggca
     8641 gtgcgcaaca tggtcgagtt gacgggcgta acgcctgcgg aagccattca tatggcatcg
     8701 ctgcatccgg cgcgaatgct gggtgttgat ggtgttctgg gatcgcttaa accgggcaaa
     8761 cgcgccagca tcgttgcgct ggatagcggg ctgcatgtgc aacaaatctg gattcagagt
     8821 caattagctt cgttttgata gtttgctcct ttattgggcc ttcacttccc ccgtaaggcc
     8881 tttctttttc tttcgttttg atctgtgcag cggtgtcgga tgcgacgcta acgcgtctta
     8941 tgcgacctac ggtacgccgc atccagcaat cggtgaacat agcgacgtca ttacgcaatt
     9001 ctttaatctt cccttgtctt ttctttcaat gatcacagat ttcgttttat ttcctttttc
     9061 tccattgaac tttcggtttc ttttctatag attttaatca acgaaagaca tcaccaagtg
     9121 aaatgaaacg aaaggcaagt gaaagcgaca acgcccgacg tcaagttcat cagactaagg
     9181 attgagttat gccaaaaaat tacacccctg ctgccgccgc aaccggtaca tggactgaag
     9241 aagagatccg ccatcagcct cgcgcatgga tccgttcact caccaacatc gacgcgctac
     9301 gttccgcgct caataacttc cttgaaccgt tactgcacaa agagaatctg cggatcatcc
     9361 tgaccggagc cggaacctcg gcatttatcg gtgacatcat cgcgccgtgg ctcgccagcc
     9421 ataccggtaa aaacttcagc gccgtaccga ccaccgatct ggtcactaat ccgatggact
     9481 acctgaaccc agcccatccg ctgctgttga tctccttcgg tcgatccggc aacagcccgg
     9541 aaagcgtcgc agccgtggaa ctggcaaatc aatttgtacc ggaatgctat cacctgccga
     9601 tcacctgcaa cgaagcgggc gctctttacc aaaacgcgat caacagcgat aacgcgtttg
     9661 ccctgctgat gcccgcagaa acgcacgatc gcggctttgc gatgaccagc agcattacca
     9721 ccatgatggc cagctgcctc gcggttttcg cacctgagac gatcaacagc caaaccttcc
     9781 gcgacgtggc ggatcgttgc caggcgatcc tgacctcact gggcgatttc agcgaaggtg
     9841 tgtttggtta cgcaccgtgg aaacggatcg tttatctcgg tagcggtggc ttacagggcg
     9901 cagcacgcga gtcggcgctg aaagtgctgg aactgacggc gggtaaactg gcggcctttt
     9961 atgattctcc aaccggattc cgtcatggac caaaatcgct ggtcgatgac gaaacgctgg
    10021 tggtggtatt tgtctccagc cacccttaca cccgtcagta tgatcttgat ctgctggctg
    10081 aacttcgccg tgacaaccag gcaatgcgtg taatcgccat cgccgcggaa agcagcgaca
    10141 tcgtcgctgc cggtccacat atcatcctgc caccgtcacg tcactttatc gacgttgagc
    10201 aggcattttg cttcctgatg tacgcccaga cgtttgcact gatgcagtcg ctgcacatgg
    10261 gcaatacgcc ggatacccca tcagccagtg gcaccgttaa ccgcgtggtg caaggcgtaa
    10321 tcattcatcc gtggcaggca taagaggatc gcattatgag cattatctcc actaaatatc
    10381 tgttacagga cgcccaggcc aatggctacg cggtgcctgc ttttaacatt cataacgccg
    10441 agacgatcca agcgatcctc gaagtgtgca gtgaaatgcg atcgccggtg atcctcgccg
    10501 gaacgccggg gacctttaaa cacatcgcgc tggaagagat ctacgccctg tgtagcgcct
    10561 attccacaac ctacaacatg ccactggcgc tgcatctcga ccaccacgaa tcgctggatg
    10621 atattcgccg taaagtccac gcaggtgtac gcagtgcgat gatcgacggc agccacttcc
    10681 cgtttgccga gaacgtgaag ctggtgaaat cggttgttga cttctgccac tcacaagatt
    10741 gcagcgtgga agcagaactg ggccgcctgg gcggtgttga agatgacatg agcgttgacg
    10801 ccgaaagtgc attcctgacc gatccacaag aagctaaacg ctttgtcgaa ctgactggcg
    10861 tcgacagcct ggcggtagcg attggtacgg cgcacggctt atacagcaaa acgccgaaga
    10921 ttgatttcca gcggctggcg gaaattcgtg aagtggtgga tgttcctctg gtgctgcatg
    10981 gtgccagcga tgttccggat gaatttgtcc gtcgcactat tgaacttggc gtcacaaaag
    11041 tgaacgttgc cacagaatta aaaatagcct tcgctggcgc ggttaaagcc tggtttgcgg
    11101 aaaatccgca gggtaatgat cctcgttatt atatgcgcgt cggaatggat gcgatgaaag
    11161 aagttgtcag aaataaaatt aatgtctgtg gttcagcgaa tcgaatttca gcataatcat
    11221 tagtttttta tgatttatcc caatgtactt cccgattaat atcggggagt gccttaatgg
    11281 aaaaggagat aactaaacct taataaatac atcagtacaa tatcgcaaca ata
//
LOCUS       DAPGWD010000129        11328 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPGWD010000129 DAPGWD010000000
VERSION     DAPGWD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.130"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            223..723
                     /gene="tssB"
                     /locus_tag="RHX88_003402"
     CDS             223..723
                     /gene="tssB"
                     /locus_tag="RHX88_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDV2232057.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDIGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            758..982
                     /locus_tag="RHX88_003403"
     CDS             758..982
                     /locus_tag="RHX88_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232058.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1033..2508
                     /gene="tssC"
                     /locus_tag="RHX88_003404"
     CDS             1033..2508
                     /gene="tssC"
                     /locus_tag="RHX88_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDV2232059.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYHEDVSRNHEDYL
                     WGNTAWMLACNVADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAR
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2515..2928
                     /gene="tssE"
                     /locus_tag="RHX88_003405"
     CDS             2515..2928
                     /gene="tssE"
                     /locus_tag="RHX88_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDV2232060.1"
                     /translation="MIPDRERTTGSLFERMEASPARNWQGSSIHALRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSLAIAQCIERYEPRISHAEVQAVVSSATSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2932..4782
                     /gene="tssF"
                     /locus_tag="RHX88_003406"
     CDS             2932..4782
                     /gene="tssF"
                     /locus_tag="RHX88_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HDV2232061.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRNQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGSPALPDGL
                     QGESFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4746..5828
                     /gene="tssG"
                     /locus_tag="RHX88_003407"
     CDS             4746..5828
                     /gene="tssG"
                     /locus_tag="RHX88_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HDV2232062.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5853..7133
                     /gene="tagH"
                     /locus_tag="RHX88_003408"
     CDS             5853..7133
                     /gene="tagH"
                     /locus_tag="RHX88_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HDV2232063.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7130..7654
                     /gene="tssJ"
                     /locus_tag="RHX88_003409"
     CDS             7130..7654
                     /gene="tssJ"
                     /locus_tag="RHX88_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HDV2232064.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7657..8988
                     /gene="tssK"
                     /locus_tag="RHX88_003410"
     CDS             7657..8988
                     /gene="tssK"
                     /locus_tag="RHX88_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HDV2232065.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRTGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8993..9754
                     /gene="icmH"
                     /locus_tag="RHX88_003411"
     CDS             8993..9754
                     /gene="icmH"
                     /locus_tag="RHX88_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HDV2232066.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9763..>11328
                     /locus_tag="RHX88_003412"
     CDS             9763..>11328
                     /locus_tag="RHX88_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2232067.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQ"
BASE COUNT         2649 a         3075 c         3078 g         2526 t
ORIGIN      
        1 gaattgcaca ggttcacaca atctatttaa cgcttgcaga atacccctct ttacgatcga
       61 taaaaagtcc ttttcttatt tattttgaag cgtaaaatgc ggggtgatga aggagtggtg
      121 attttatttt caccgccatt agggcggcat tgccgatatt tttttgcggt acaaatatgc
      181 cgtgttcatg gcctgtacga gataacatga cggatcactt ttatgagcaa aaaatttgaa
      241 ggcagcgtcg cgcctcgcga gcgtattaat atatcgtatg tcccaaaaac agatggccag
      301 acggctgaag tggaacttcc gcttaacatg ctcgttgtcg gcgatatcgg aaatacgcag
      361 gaaacatccc cactggatga acgtcaggca gtttccgtta acaagcacaa tttcggtgca
      421 gtgatggctg aagcagccat tggcctcaat tttactgtcc ccgccacgct gaagggaagc
      481 acaacagacg atgaactgaa tgtggcgctg aatatcaaat cactggatga tttttcacca
      541 gatagcgtgg cccggcaggt tccggaagtg aataaattgc tggaactgcg tgaagcactg
      601 actgcactga aggggccgat gggtaacctt ccggcattcc gtacccagct ccaggcattg
      661 ctggaaaatg aagagtcccg cgagcagttg ctcaaagaaa tcggccaggt gagcaataaa
      721 taaccaccac tatttcatta aacagggtta tccaggcatg acggcacaca aaaatatctc
      781 cgggacatac cacctctctg ttgcagacat tctgcaggtg gtttaccagg tatgtttcag
      841 cccgtccgtt gaaatcaacc aggatggtgt ggctgcgctg attacaacac tggatcgccg
      901 tatcagtgat ctattggatg agattatcca tttttgtgag tttcagcaga gcgcgtcgca
      961 ctggcagcgc gtacttcact gacggcgaaa agccggtttc agaaacacag ctaaaacaga
     1021 aggattttca gtatgtcttt acaggaagag gaactcgttt ccagtcatgc cgggcagcct
     1081 gagcaggcat cgtcgctgct tgatcaaatc atggcacaga cccgtattca gcccggttcg
     1141 gaaggttatg acgttgcccg ccagggtgtg acagccttta ttgccagcat cctgcaatcc
     1201 actgcatctg ccgaaccggt caataaactg gccgtggaca gcatgattgc agatatagat
     1261 gaacgcatca gccgccagat ggatgtcatc atccatgcgc ctgcctttca gcaggtcgag
     1321 tcgttctggc gctcactgaa aaccatggtg gatcgcgttg atttccgtga aaacatcaag
     1381 gtcaacgtgc ttcatgtcac gaagcaggaa cttctggaag actttgaatt tgccccagaa
     1441 attattcagt ccggatttta taaacacgtt tattcgtccg gattcggaca gtttggtggt
     1501 gaacccatcg ctgccgtgct gggcgcttat gagttcaaaa ataccgcacc tgacatgaaa
     1561 ctgctgcaat acgtcagtgc tgtgggcgca atggcgcacg caccgttcct gtcctccgtt
     1621 tccccggagt ttatggggct gaactcgtgg accgaactgc ctaatatcaa agatctttat
     1681 gccatctttg aaggtccggc ctacaccaaa tggcgcgccc tgcgtgactc ggaagattcg
     1741 cgctatttag ggcttacggc accccgcttc ctgcttcgcc agccttattc accaacagat
     1801 aaccctgtta agaactttaa ctatcacgaa gatgtcagcc ggaatcacga agattatctg
     1861 tggggtaata cggcctggat gctggcgtgc aatgtcgcag acagttttgc caaataccgc
     1921 tggtgtccaa atatcatcgg tccgcaaagc ggcggtgcag tgaaagatct gccggtgcat
     1981 ctgttcgaaa cgatgggtca gattcaggcc aaaatcccaa ccgaggtgct ggtcaccgat
     2041 cgccgcgaat ttgaactggc tgaagagggt tttatcaccc ttaccatgcg taaagactct
     2101 gataatgcag cctttttctc tgcaaactcg gtacaaaaac cgaagcactt tccgggtaaa
     2161 gacgcagaaa ccaactataa gttgggtacg cagcttcctt atctcttcat catcaatcgg
     2221 ttagcgcact acatcaaggt gttgcagcgt gagcaactgg ggtcatggaa agaaaggagc
     2281 gacttagaga gggagcttaa tacctggatc cgtcagtatg ttgccgacca ggaaaacccg
     2341 cctgcggacg tgcgcagccg caagcctctg cgtgctgcca gagttgaggt tatggatgtg
     2401 gaaggcgaac ccggctggta ccaggtcgcg ctgagcgtgc ggccccattt taaatttatg
     2461 ggggcaaact ttgagctttc cctggttggc cggttagaca gggagtaaca cgccatgata
     2521 ccggatcggg aaagaaccac aggttctctt tttgagcgta tggaagcctc accggccaga
     2581 aactggcagg gaagcagtat tcatgcgctc aggcagtcca ttcgccagaa cctgcgaaac
     2641 attcttaata cccggtccgg tagttgccgg ggtgcgcctg aactgggcat tgatgaaccg
     2701 gaaggggctg aaaacttccg cgaatcaatg agtctggcca tcgcgcaatg tatagaacgc
     2761 tatgaacccc ggatctcaca cgctgaagtg caggctgtgg tttcatccgc caccagcccg
     2821 ctggatatga cgtttcatat tacagcctgg gtgacattta acgaaacaca tgaagtgctt
     2881 gaatttgata tggcaccgaa tggcagccag cattatcggg tggactgaca gatggaattt
     2941 gaagaacgct atttccggga agaactcgat tacctgcgcc agctcagcaa gctgctggca
     3001 acggaaaaac cccatcttgc ccgcttcctg gccgaaaaag atgcggatcc ggatattgaa
     3061 cgcctgctgg aaggcgtggc ttttcttacc ggcaatctcc gccagaaaat tgaggatgaa
     3121 ttcccagaac tgacgcacgg gcttattaag atgctctggc ctaattacct gcgtccggtt
     3181 ccggcaatga cccttattga atatacgccg gatatggata agtcttctgt gccggtgtta
     3241 atcccccgta atgagcagtt tacaaccaac gccggggaaa tcagagtcga tgaagtgctg
     3301 ccctccgatg ctaaaaagga ggagccgcct ccctgtacct tcaccctctg ccgggatatc
     3361 tggctgctgc ccgttcgcct ggagcagatt gaaaaccgca gtacgacccg taatggtgtt
     3421 atcaacatca ccttttcggt cgcaccggga acggacttcc gcacgctgga tctgaacaaa
     3481 cttcgcttct ggctcggcaa tgacgacaac tatacccgta accagcttta tttatggttc
     3541 tgtgaatact tgcagggtgc cgacctgact gtgggtgaac agcatattcg cctgcctgag
     3601 tttatgctga aagctgtcgg ttttgagccg caggatgcca tgctgccttg gccgaaaaac
     3661 gtccacagcg gctaccggat ccttcaggag tatttctgtt acccggatgc gtttctcttt
     3721 tttgatcttt gtggttcccc ggcgttgcct gacggattgc agggtgaatc ctttaccctg
     3781 caactgcgtt tttcgcgccc attgcccgtg gacatccggt tgcgccgcga ctccctgcgc
     3841 ctgtattgcg cgcctgccat taatttattt atccaccatg cagaagccat cacgctggac
     3901 aaccggcggg cagactatcc gctggttccc agccgccatt atccggaaca ttacgatgtc
     3961 ttttccgtta acggcgtgat aagccaggtg caggatatgt tcaggaaaaa agatctgggg
     4021 cgtcctgttt cgacgcaggc cgcgcgccag tggcctgcct ttgaaagttt cagccatcag
     4081 atggaataca gccggaagcg ggaagtggtg tactggcatc accggaccaa aacatccctg
     4141 ttccatcgcg gctttgatca tacccttgct tttatacatg ctgatggcag ttatccgtca
     4201 gacgaatcgc tgctcagtaa tgaagtggtt tcggtatcgc tgacctgtac caaccgtgag
     4261 cttccgtcac aaattcgttc cggcgatatc accggcacaa ccggtaaaaa tgcggctgtt
     4321 gcttcatttc gcaacattac ccgcccgacg caaccgctct ggccggtcat tgatggcagc
     4381 ctgcactggt ccctgctctc cgccatgaac ctgaattatc tgtcattact ggatacggac
     4441 gcgctgaagc aggtcatcgc caactttgat cgccacgcaa tccatcatcc gcagacggcg
     4501 cggctgtcac aacaaaagct ggatgccatt gagcgtctgg agacccgccc tgtcgatcgc
     4561 ctgtttacgg gcattcccgt ccgggggctg gcctccacgc tgtatctgca cccggagccg
     4621 tttgtctgtg aaggggaaat gtatctgctc ggtacggtgc tttcgcattt tctgtcgctg
     4681 tacgccagcg ttaactcatt ccacatgctg accgttgtga acacagaaag ccaggagaca
     4741 tggaaatgga cggaaagaac cgggcagcat cctcttatct gagtccggga aatcccccgg
     4801 cagataaaga gcagaatgat ccgctggcac aggttttcca taatgcctgc tcttacaatt
     4861 tttttgcgat ggcggagctg ttgcaccgcc tggcaaaggg tgaaaagggg acgccagaat
     4921 tatccctgcg cgacgatccg gcacaggaaa ccctgcgttt cagcgccgat gccagccttg
     4981 ccttcccgtg cagtgatatc agtgcgctga aacgggatac gtcaggggca ttcaggatga
     5041 ccaccacctt tatggggtta caggggagcc agtcgcctct ccccggttat tacctcgatc
     5101 acctggcctg gaaagcggta catgaacaaa gtccggtagg tgattttctg gatatgttca
     5161 gccaccgcct gacccagttt gtctggcata tctggcgtaa gtaccgctat cacatcagtt
     5221 ttcgtaacgg cggtgtggat gcgttctcgc agcgcatgta ctcccttgtc gggctgggcc
     5281 accgccagct tcgcgataag ctggcgatta atcacagcaa aatgctggcg tattccggga
     5341 tcctggcaaa tccgggacga tccccggaaa ttatctgtgg cctggtgtcc cactgctttg
     5401 atttgtcaga agtgacgctg caaaactggc aacgtcgcaa ggtggatatt gaaccggatc
     5461 agcaaaatag tctggggagc tattctctta aaaatggcga aaaactggcg ggaagatcgg
     5521 tactgggcaa ctttgttctg ggtacacgag tgcccgatct cagcggaaaa ttccagctca
     5581 gtattaccag cctgacccga aaacagttcc tctcttttct gccgtccggc gaaaacttcc
     5641 tgccactgac gatgttcgtg tccttcatcc tgcgcgatca gcttgcctgg gacttacatc
     5701 tggggctggc gccggagcag gtgggcgcaa tgcgcctggg cgataacaaa agtgcgctgc
     5761 tgggctggac cagcttcctc ggcacaccgg aagaacgacc ctcagtcacc atcagggtac
     5821 ggtcgtaaat tatgataatc acggagcaga atatgccgga agagaaacta cagacgcttt
     5881 cattgcaggt cattaacggc agtgagctgg aaagcgggcg ggcggcgcgc tgtctgttca
     5941 cacagcaggg aaatgtcggc catggccccg aatgccactg gtcggtacag gatcgtcagc
     6001 agagcattcc ggcccaggct tttaccgtta tcctgcacga tggcacattt tgtctgcgcc
     6061 cgcagacggc acaactgtgg ctgaatcagg caaaagtcac agcaacatca gacctaatac
     6121 agttgcgcca gggcgatgag atccagatcg gacggctgat ggtgagggtt catctgaacc
     6181 ggggagatat tccccattac gatgaggaaa tggccactcc cgaaaccatc gttaccaatc
     6241 gcgatatgct cacggatacc ctgctatcaa cggagggtgc gccacactat ccgggaatga
     6301 ctcaccggca ccagcttgca gacaccgtgg taaacggttt ttctgccgat ccactccagg
     6361 cacttcagtc cgaaagcctg attaccacgg gcgatccgct ttcaggcatt gcggctgtcc
     6421 ggccatcggc accgctgtcc gatccggcaa gtaatggggg gatcaatact ccgtttatgg
     6481 atctgccgcc catttatgcc agccccggcg atcgtaatga cgacgtctct gcggcagaaa
     6541 tggcgcaacg ccaccttgcg gtcaccccct tacttcgcgg tcttggcggc tcgcttacca
     6601 tgagcaattc cgacgatgcg gatgattttc tggaggaggc cggacgaacg ttacaggccg
     6661 caataaaagg tctgctcgat ttgcagcagc agcgtaacag cctctcagac aaacatttgc
     6721 gcccgctgga agataacccg ctgcgcctga acatggatta cgccaccgcg ctcgacgtaa
     6781 tgtttgccga aggtaaaagc ccggtacatc tggcggctcc cgccgccgtc agtgaaagcc
     6841 tgcgcaatgt ccgccaccac gaagaagcta accgggcagc aattgtggag tcgcttcgtg
     6901 tcctgctgga tgctttctca ccacaaaatc tgctgcgccg ctttgtgcag taccgccgca
     6961 gccatgaact gcgccagccg ctggatgatg ccggagcatg gcaaatgtac agccattatt
     7021 acgaagaact ggcctccgat cgccagcagg ggtttgagat gctgtttaac gaggtctacg
     7081 cccaggtcta tgaccgggtg cttcgtgaaa aacagcggga gccggaagca tgaacaggaa
     7141 ggcgtttctg gcctgcgtgc tgatgtgcgt catattaacc ggctgcgaaa cggcgaaaaa
     7201 aatcagcgag gtgatcaaaa acccggatat ccaggtcggg agcctgaagg agcaaccttc
     7261 ggaaataacc gtcaccctgc tgaccgaacc ggacaccaat accaatgcgg agggtgaatc
     7321 agccgccgtg gatgtgcaac tggtgtacct gactgacgac tcaaaattgc tggcagccga
     7381 ctatgaccag atagccagta cgccgctacc ggatgtgttg ggtaaaaact atatcgacca
     7441 ccaggatttc aacctgttgc cggacaccat caaaaccctg ccgccggtaa agctggatga
     7501 gaaaacgcag tttattggtg tcgtggccta tttctccgat gaccaggcca ctgaatggaa
     7561 acaaatcgag acggtggaag gaaccggcca ccattaccgc ctgctggtgc atgtacgtca
     7621 aagcagcatc gaaatgaaaa aagaggatga ataattatgg caaccacccg caacaaggtg
     7681 atgtggcagg aagggatgct gatgcgccca caccatttcc agcaacagca gcgttacaac
     7741 gactacctgg ataaccagcg tttccgggcc atgaatgatt tatcctgggg atttaccgaa
     7801 ctcaccctca acaatgaact gctggcgcag ggtaagatca tgattgacag cgcgtcaggc
     7861 acactgcccg acggcaccgt cttttctatc cccgaccagg acgcactgcc cgatccgctg
     7921 cacccgcaaa attttccgga cgaaagaagc cgcaatatct acctcgctct gccggtcgcc
     7981 agtgatgtga gaaatgaaat cagcgacgga cggcgaaccg ggcgttaccg gctgaattat
     8041 gccgatgtcc gggatttgca ttcagaagaa ggtgacgcgc gaacattgac gctgggtcaa
     8101 ctgacgccgc gcattatgag cggtgcagaa gatatgagcg cctatattac gctcccgctt
     8161 tgccgtatca gcgatcgcca tgccgatggc tccctgacgt tggatgacga ttttatcccc
     8221 tcctgccaga atattcaggt cagtaagaaa ctgcgtgttt atctcaaaga ggtacagggg
     8281 gccattggcg gacgggcaag cgatctggca aaccgtattg gctctccggc gcagagcggc
     8341 atcgcggatg tggcggaatt tatgatgttg cagttactta accgtaacca gacccggttt
     8401 acccatcgcg cccgccgatc ccagctccac ccggaagatt tctaccttga tcttgctggg
     8461 ttgctgggtg aactgatgac ctttaccgag ccgtcgcgcc tgccctgccc gcttgatgtg
     8521 tatgatcatc atgatctgac caaaacattt aaaacactgt taccggaagt caaacgggcg
     8581 ctgcataccg tactgtcgcc aagagcggtc aatctgccgc tgcatctgcg tgacggtatc
     8641 tggcaggccg atatccatga cacagaactg ctgcaatctg ccacctttgt gctggccgtg
     8701 gcggcaaacg tgccggtcga tcagatccag cgtcagttta tccagcagtc gaaaatttcc
     8761 tcgccggaaa aaatccgcaa tatggtcagt gtgcagatcc ccggtattcc attgcgtgcc
     8821 cttatggtgg ctccccgcca gcttccttac cattccgggt tcagctattt cgaactcgac
     8881 aagagtggcc aggcctggac agaaatggct gccgccgggg ccgttgcact gcatgtttcc
     8941 ggcagtttcc cggatctgaa catgcaactg tgggcgataa gagggtaaga cgatggatga
     9001 ggggtcactg tctctgccac catttaccgg acacgacgaa aaaagccagc gcaactacca
     9061 tctcgcgctt cgtggcaaca gcctgaatcc gatgatcgac gcggccacgc cgctactcgg
     9121 tatggtaatg cgcctttcaa ccatgaacag ccagacgatg ccggagcatc tttttgctca
     9181 ggtggtgaca gatgttcagg ccgtggagca actgcttcag gagcagggct acgaaccggg
     9241 cgtgattatc tcgtttcgct acatcctctg cacgtttatt gatgaagcgg cgctcggtaa
     9301 cggctggtca aacaagaacg agtggatcaa gcagtcattg ctggtccatt tccacaacga
     9361 agcctggggt ggcgagaaag ttttcatcct gctggagcgc ctgatccgtg aacccaaacg
     9421 ttaccaggat ctgctggaat ttctctggat ctgtttttct ctgggatttc gcgggcgcta
     9481 caaagtggca gcacaggatc agggggaatt tgagcagatc taccgccgtc tgtaccacgt
     9541 tctgcataaa ctgcgcggcg atgccccttt cccgctgtta caccaggaca aaaaaaccca
     9601 gggcgggcgt tatcagctca tcagtcgcct gactgttaaa catatttttt gtggtggcgt
     9661 tgtcgtgctg gcgttgtttt acctgttcta tctgttgcgg ctggacagcc agacgcagga
     9721 catcctgcac cagctaaaca aattacttcg ataaggacgt ttatgatcca gattgatctt
     9781 cccacgctgg taaaacggct gaacctgttc tcccgccagg cgctggagat ggccgcctct
     9841 gaatgtatga gtcagcaggc agcggaaatt accgtcagcc atgtgctcat tcagatgctc
     9901 gccatgccac gcagtgacct gcgggttatt acccgccagg gcgatattgg catggaagag
     9961 ttgcgccagg cgctgacggt agagaactac acaaccgccc gttctgcgga cagctacccg
    10021 gcgttttccc cgatgctggt tgagtggctt aaagagggct ggctgctggc gtcggctgag
    10081 atgcagcaca gcgaactgcg cggcggcgtg ttgctgctgg ccctgctgca ttcgccgctg
    10141 cgttatatac cgcctgctgc cgcccggctg ttgaccggca ttaaccgtga ccgtctgcaa
    10201 caggactttg tgcagtggac acaggagtcg gcggaatcag tcgtgccgga tgcagacggt
    10261 aaaggcgcag gcacaatgac ggacgcctct gacaccctgc ttgcccgcta tgccaaaaac
    10321 atgaccgcag acgcccgtaa cggcaggctt gacccggtac tgtgccgcga ccacgaaatc
    10381 gacctgatga tcgacattct ctgccgccgc cgtaaaaaca acccggtggt ggtgggcgaa
    10441 gcgggcgtgg gcaaaagcgc actgattgaa gggctggcgc tgcgcatcgt ggcaggccag
    10501 gtgccggaca agctgaaaaa caccgatatc atgacccttg acctgggcgc attgcaggcc
    10561 ggggcgtcgg tgaagggtga attcgaaaaa cgtttcaaag ggctgatggc ggaggtcatt
    10621 tcctccccgg tgccggtcat tctgtttatc gacgaagcac ataccctgat tggcgcgggc
    10681 aaccagcagg gcgggctgga tatctccaac ctgctcaaac cggcgctggc gcgcggcgag
    10741 ctgaaaacca tcgccgccac cacctggagc gagtacaaaa aatacttcga aaaagatgcc
    10801 gccctgtcgc gccgcttcca gttggtgaag gtcagcgaac ccaacgctgc cgaagccacc
    10861 attattctgc gcggtctgtc ggcggtctat gaacagtctc acggcgtgct gattgatgat
    10921 gacgccttgc aggccgctgc gacattaagc gagcgttatc tctccgggcg tcagttaccg
    10981 gacaaagcga ttgatgtgct ggataccgcc tgcgcccgtg tggccatcaa cctgtcgtcg
    11041 ccgccgaagc aaatctcggc gctgaccact ctgagccacc agcaggaggc ggaaattcgc
    11101 cagcttgagc gcgagcttcg catcggactg cgtaccgaca catcacggat gaccgaggtg
    11161 ctggtgcagt atgatgaaac gctgacggcg ctggatgaac tggaagcggc ctggcaccag
    11221 cagcagacgc tggtccggga gattattgcg ctgcgccagc agttactggg cgtggcagag
    11281 gacgatgcgg cgccgttgcc ggacgcagat accgtggagg atacgcag
//
LOCUS       DAPGWD010000130        11309 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPGWD010000130 DAPGWD010000000
VERSION     DAPGWD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.131"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            73..168
                     /locus_tag="RHX88_003413"
     CDS             73..168
                     /locus_tag="RHX88_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232068.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            332..3007
                     /gene="acnA"
                     /locus_tag="RHX88_003414"
     CDS             332..3007
                     /gene="acnA"
                     /locus_tag="RHX88_003414"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDV2232069.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3071..3661)
                     /gene="ribA"
                     /locus_tag="RHX88_003415"
     CDS             complement(3071..3661)
                     /gene="ribA"
                     /locus_tag="RHX88_003415"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDV2232070.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            3831..4595
                     /gene="pgpB"
                     /locus_tag="RHX88_003416"
     CDS             3831..4595
                     /gene="pgpB"
                     /locus_tag="RHX88_003416"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDV2232071.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            4744..5052
                     /gene="lapA"
                     /locus_tag="RHX88_003417"
     CDS             4744..5052
                     /gene="lapA"
                     /locus_tag="RHX88_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDV2232072.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5059..6228
                     /gene="lapB"
                     /locus_tag="RHX88_003418"
     CDS             5059..6228
                     /gene="lapB"
                     /locus_tag="RHX88_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDV2232073.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6421..7158
                     /gene="pyrF"
                     /locus_tag="RHX88_003419"
     CDS             6421..7158
                     /gene="pyrF"
                     /locus_tag="RHX88_003419"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDV2232074.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7158..7484
                     /gene="yciH"
                     /locus_tag="RHX88_003420"
     CDS             7158..7484
                     /gene="yciH"
                     /locus_tag="RHX88_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDV2232075.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQFQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(7610..7828)
                     /gene="osmB"
                     /locus_tag="RHX88_003421"
     CDS             complement(7610..7828)
                     /gene="osmB"
                     /locus_tag="RHX88_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDV2232076.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8097..8846)
                     /locus_tag="RHX88_003422"
     CDS             complement(8097..8846)
                     /locus_tag="RHX88_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2232077.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(8936..9109)
                     /locus_tag="RHX88_003423"
     CDS             complement(8936..9109)
                     /locus_tag="RHX88_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232078.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(9257..11242)
                     /gene="pdeR"
                     /locus_tag="RHX88_003424"
     CDS             complement(9257..11242)
                     /gene="pdeR"
                     /locus_tag="RHX88_003424"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV2232079.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         2853 a         2687 c         2899 g         2870 t
ORIGIN      
        1 cttttaatac accgcagcaa taacagcttg agttatctca acacaaaata ataaccgtta
       61 agggtgtagc ctatgatcaa cacaaatatg aaatattggt cctggatggg cgcgttttct
      121 ctgtcgatgc tcttctgggc cgaactcctc tggatcatta ctcactgatc cttgaccccg
      181 ctgcggcggg gttgtcattt gctttgccac aaggtttctc ctcttttatc aatttgggtt
      241 gttatcaaat cgttacgcga tgtttgtgtt atctttaata ttcaccctga agagaatcag
      301 ggcttcgcaa ccctgtcatt aaggaggagc tatgtcgtca accctacgag aagccagtaa
      361 ggacacgttg caggccaaag ataaaactta ccactactac agcctgccgc ttgctgctaa
      421 atcactgggc gatatcaccc gtctacccaa gtcactcaaa gttttgctcg aaaacctgct
      481 gcgctggcag gatggtaact cggttaccga agaggatatc cacgcgctgg caggatggct
      541 gaaaaatgcc catgctgacc gtgaaattgc ctaccgcccg gcaagggtgc tgatgcagga
      601 ctttaccggc gtacctgccg ttgttgatct ggcggcaatg cgcgaagcgg ttaaacgcct
      661 cggcggcgat actgcaaagg ttaacccgct ctcaccggtc gacctggtca ttgaccactc
      721 ggtgaccgtc gatcgttttg gtgatgatga ggcatttgaa gaaaacgtac gcctggaaat
      781 ggagcgcaac cacgaacgtt atgtgttcct gaaatgggga aaacaagcgt tcagtcggtt
      841 tagcgtcgtg ccgccaggca caggcatttg ccatcaggtt aacctcgaat atctcggcaa
      901 agcagtgtgg agtgaattgc aggacggtga atggattgct tatccggata cactcgttgg
      961 tactgactcg cacaccacta tgatcaacgg ccttggcgtg ctggggtggg gcgtgggtgg
     1021 gatcgaagca gaagccgcaa tgttaggcca gccggtttcc atgcttatcc cggacgtagt
     1081 gggctttaaa cttaccggta aattacgtga aggtattacc gccacagacc tggttctcac
     1141 tgttacccaa atgctgcgca aacatggcgt ggtggggaaa ttcgtcgaat tttatggtga
     1201 tggtctggat tcactaccgc tggcggatcg cgccaccatt gccaatatgt cgccagaata
     1261 tggtgccacc tgtggcttct tcccaatcga tgctgtaacc ctcgattaca tgcgtttaag
     1321 cgggcgcagc gaagatcagg ttgagttggt cgaaaaatat gccaaagcgc agggcatgtg
     1381 gcgtaacccg ggcgatgaac cgatttttac cagtacgtta gaactggata tgaatgacgt
     1441 tgaagcgagc ctggcagggc caaaacgccc acaggatcgc gttgcactgc ccgatgtacc
     1501 aaaagcattt gccgccagta acgaactgga agtgaatgcc acgcataaag atcgccagcc
     1561 ggtcgattat gttatgaacg gacatcagta tcagttacct gatggcgctg tggtcattgc
     1621 tgcgataacc tcgtgcacca acacctctaa cccaagtgtg ctgatggccg caggcttgct
     1681 ggcgaaaaaa gccgtaactc tgggcctcaa gcggcaaccg tgggtcaaag cgtcgctggc
     1741 accgggttcg aaagtcgttt ctgattatct ggcaaaagcg aaactgacac cgtatctcga
     1801 cgaactgggg tttaaccttg tgggatacgg ttgtaccacc tgtattggta actctgggcc
     1861 gctgcccgat cctatcgaaa cggcaatcaa aaaaggcgat ttaaccgtcg gtgcggtgct
     1921 gtccggcaac cgtaactttg aaggccgtat ccatccgctg gttaaaacta actggctggc
     1981 ctcgccgccg ctggtggttg cctatgcgct ggcgggaaat atgaatatca acctggctgc
     2041 tgagcctatt ggtcatgatc gtaaaggcga cccggtttat ctgaaagata tctggccatc
     2101 ggcacaagaa attgcccgtg cggtagaaca agtctccaca gaaatgttcc gcaaagagta
     2161 cgcagaagtt tttgaaggca cagcagagtg gaaggaaatt aacgtcacac gatccgatac
     2221 ctacggttgg caggaggact caacctatat tcgcttatcg cctttctttg atgaaatgca
     2281 ggcaacacca gcaccagtgg aagatattca cggtgcgcgg atcctcgcaa tgctggggga
     2341 ttcagtcacc actgaccata tctctccggc gggcagtatt aagcccgaca gcccagcggg
     2401 tcgatatcta caaggtcggg gtgttgagcg aaaagacttt aactcctacg gttcgcggcg
     2461 tggtaaccat gaagtgatga tgcgcggcac cttcgccaat attcgcatcc gtaatgaaat
     2521 ggtgcctggc gttgaagggg ggatgacgcg gcatttacct gacagcgacg tagtctctat
     2581 ttatgatgct gcgatgcgct ataagcagga acaaacgccg ctggcggtga ttgccgggaa
     2641 agagtatgga tcaggctcaa gccgtgactg ggcggcaaaa ggtcctcgtc tgcttggaat
     2701 tcgtgtggtg attgccgaat cgtttgaacg aattcaccgt tcgaatttaa ttggcatggg
     2761 catcctgccg ctggaatttc cgcaaggcgt aacgcgtaaa acgttaggac taaccgggga
     2821 agagaagatt gatattggcg atctgcaaaa tctacaaccc ggtgcgacgg ttccggtgac
     2881 gcttacgcgc gcggatggta gccaggaggt cgtaccctgc cgttgtcgta tcgacaccgc
     2941 gacggagttg acctactacc agaacgacgg cattttgcat tatgtcattc gtaatatgtt
     3001 gaagtaacaa gtatttgctt gccggttatt ttgcttccgg caagcaaact aattacacaa
     3061 tgcaagaggg ttatttgttc agcaaatggc ccattttctc ggctttggta tcgagataat
     3121 gttcgttatt ggggttacga cctacaatca atggtacgcg ttcaacaata ttaatccctg
     3181 cttcggtcag aatttcgact tttttcgggt tattggttaa caagcggact tcattgacgc
     3241 caaggagttt gaacatatca gcgcaaagag tgaagtcgcg ctcatcagcg gcgaagccta
     3301 actggtggtt agcctctacg gtatcgtaac cttgatcctg cagtgcgtaa gcgcggattt
     3361 tattcagcag accaatgtta cgaccttcct gacggtgata cagcaaaata ccacggcctt
     3421 cctcggcaat ttgcgtcaat gccgcttcga gctggaagcc acaatcgcag cgcaagctga
     3481 acagggcgtc accggtcaga cattcggaat ggacgcgcgc aagtaccggg gtatgcccgg
     3541 aaatatcgcc atagactagc gcgacatgat cgtgtccggt tgccagttct tcaaatccca
     3601 ccatcaggaa atcgccccat ggggttggca gtttggcttc tgccacacgt ttaagctgca
     3661 tgaaattctc cagataatgc tggttctgta ttggcttatt ttgccataac gagaaggggt
     3721 tcacctaatc acgacgcgtc gatcgttcac ggaatggcac aaatctgtca atttttcctg
     3781 gaactggcgt tttcagttat gattgtggga cttatcaaaa aggagaggcc atgcgttcga
     3841 ttgccagacg taccgcagtg ggagctgcac tattgcttgt catgccagta gccgtatgga
     3901 tttctggctg gcgttggcaa cctggagaac aaagttggct actaaaagcg gctttttggg
     3961 ttactgaaac tgtcacccag ccctggggcg tcattacaca tttgatttta ttcggctggt
     4021 ttctctggtg tctgcgtttt cgcattaagg ctgcctttgt attatttgcc attctggcgg
     4081 ccgcaatcct tgtgggacaa ggcgttaaat cctggatcaa agacaaagtc caggaaccac
     4141 gaccttttgt tatctggctg gaaaaaacac atcatattcc ggttgatgag ttctacactt
     4201 taaagcgagc agaacgcgga aatctagtga aagaacagtt ggctgaagag aaaaatatcc
     4261 cacaatattt gcgttcacac tggcagaaag agacggggtt tgcctttcct tccggtcaca
     4321 cgatgtttgc tgccagttgg gcactgctgg ccgttggttt gctgtggccg cgtcggcgaa
     4381 cgttaaccat tgctatcttg ctggtctggg caacgggagt catgggaagc cgcctgctgc
     4441 tcgggatgca ttggccacgc gatctggtag tagctacgtt gatttcgtgg gcgctggtgg
     4501 cggtggcaac gtggcttgcg caacgaattt gtgggccatt aacaccacct gcggaagaaa
     4561 atcgcgaaat agcgcaacga gaacaagaaa gttaacgctg gttgattttc cgaatttagc
     4621 ccttaaatca tcaacaatgc gcgtggatgc catttcgcag acggcgcgaa aatggtactt
     4681 taaagggcta ttgcggtaag ttgaccataa tttattcgct ctaaccacat aacgggaagt
     4741 aatgtgaaat atttactcat tttcttactg gtgttagcga tcttcgtgat ttcggtcacg
     4801 ttgggtgcgc agaacgatca acaggtgacg tttaattatc tgttagcgca aggggagtac
     4861 cgtatttcca cattgctggc ggtattgttt gctgcggggt ttgctatcgg ttggttgatt
     4921 tgtggcctgt tctggctgcg agttcgtgtt tccctggcgc gcgctgaacg taaaataaag
     4981 cgactggaaa accagctttc acccgcgact gacgtggctg tagtgccgca ctcgtcagcg
     5041 gcgaaggaat aactttctat gctggagttg ttgtttctgc ttttgcctgt agccgctgcc
     5101 tatggctggt atatgggccg cagaagtgcg caacaaaaca agcaagatga agccaaccgc
     5161 ttgtcgcgtg attacgtagc gggggttaac ttcctgctta gtaatcaaca ggataaagcg
     5221 gtagacctgt ttctcgatat gcttaaagag gatacaggca ccgttgaagc ccaccttacg
     5281 ctcggaaacc tgttccgttc gcgtggcgaa gttgatcgcg ctattcgcat ccatcagacc
     5341 ctaatggaaa gcgcctcgct gacctatgaa cagcgtctgt tggcgattca acaactaggg
     5401 cgtgattaca tggccgccgg gttatacgac cgcgcggaag acatgttcaa tcagctgacc
     5461 gatgaaactg acttccgcat tggcgctctg caacagttgc tacaaatcta ccaggctacc
     5521 agtgagtggc agaaagcaat tgatgttgcc gaacgcctgg tgaagctggg taaagataaa
     5581 cagcgcgtcg aaattgccca tttctactgt gagttagccc tgcagcatat ggccagcgac
     5641 gatctcgatc gtgccatgac cttgctaaaa aaaggggcgg cggcagataa aaacagcgcc
     5701 cgcgtatcca tcatgatggg acgcgtgttt atggcgaaag gagaatacgc caaagccgtc
     5761 gaaagtttgc aacgcgtgat atcccaggac agagaactgg tcagcgaaac gctggaaatg
     5821 ttgcaaacct gctatcagca gttgggtaaa actgccgaat gggcagaatt cctgcaacgc
     5881 gcggtggaag agaacaccgg tgccgatgct gaattgatgc ttgctgatat catcgaagcg
     5941 cgcgacggta gtgaggccgc acaggtctat attacgcgcc agcttcagcg tcatccgacc
     6001 atgcgtgtgt tccataagtt aatggattac cacttaaatg aagcggaaga agggcgtgcc
     6061 aaagagagcc tgatggtgct gcgtgacatg gttggcgaaa aggtacgtag taagcctcgt
     6121 tatcgctgcc agaaatgtgg ttttaccgca tacaccctct actggcattg tccgtcttgt
     6181 cgggcctggt caaccattaa accgattcgc ggtcttgatg gcctgtaatt tttaaaaaaa
     6241 tcctacttta gttacaacat actagttatt gatgttccat tgtgctccgg caacgacggc
     6301 gcagaaaaag cctgccaggg gaaaaatcgc aactgttaat tttttatttc cacgggtaga
     6361 atgctcgccg tttacctgtt tcgcgccact tccggtgccc atcatcaaga aggtctggtc
     6421 atgacgttaa ctgcttcatc ttcttcccgc gctgttacga attctcctgt ggttgttgcc
     6481 cttgattatc ataatcgtga tgacgcgctg tcctttgtcg acaagatcga cccacgcgat
     6541 tgtcgtctga aggtcggcaa agagatgttt acattgtttg ggccacagtt tgtgcgcgaa
     6601 cttcaacagc gtggttttga tatctttctt gacctgaaat tccacgatat tcctaacact
     6661 gctgcgcacg ctgtcgctgc tgcagctgac ttaggcgtgt ggatggtgaa tgttcatgcc
     6721 tctggtgggg cgcgtatgat gaccgcagcg cgtgaggcac tggttccgtt tggcaaagat
     6781 gcaccgcttt tgattgctgt gacagtgttg accagcatgg aagccagcga cctggtcgat
     6841 cttggcatga cactgtcacc tgcagattat gcagaacgtc tggcggcact gacgcaaaaa
     6901 tgtggccttg atggtgtggt gtgttctgct caggaagctg tgcgctttaa acaggtattc
     6961 ggtcaggagt tcaaactggt tacgccgggc attcgtccgc aggggagtga agctggtgac
     7021 cagcgccgca ttatgacgcc agaacaggcg ttgtcggctg gtgttgatta tatggtgatt
     7081 ggtcgcccgg taacgcaatc ggtagatcca gcgcagacgc tgaaagcgat caacgcctct
     7141 ttacagcgga gtgcatgatg agtgattcca acagccgtct ggtctactca acggaaaccg
     7201 gacgtattga tgagcccaaa gcggcccctg tacgccctaa aggcgacggt gtggtgcgta
     7261 ttcagtttca gaccagtgga cgtaaaggta agggcgtttg cctgattacc ggtgtcgatc
     7321 tcgatgatgc cgaactgaca aaacttgcag cggaactgaa gaaaaaatgc ggctgcggcg
     7381 gagcagttaa agatggagtt attgaaatcc agggcgataa gcgtgattta ttaaaatcac
     7441 tacttgaagc gaaagggatg aaggtaaaac tcgcaggtgg ttaacataaa aagccacgga
     7501 tatatccgtg gctttcgaat attttactgt gtgtattatt cagttatttc gtactgaaca
     7561 gaatcaagcg tattataatt gcgagtcgat aatcgacatt tatttacgat tatttaccga
     7621 cctggtgacc aataacacca ccgacagctg caccacctaa tgtacccaac gtactgccat
     7681 cggtcagtac tgcaccgcct aatgcccctg cacccgcgcc gattgcggtg ttgcggtccc
     7741 gtttagacca gttagaacag gcactcagag acattgccag ggtaattgcc agaacagccg
     7801 cggtcatttt tttgctcgtt acaaacataa tactctctcc tgaatttatg attcacggaa
     7861 gtaagctctc tataactata atagctaaga ataagcctgg tgaaattatt ccgtgaaatc
     7921 tgctcgcgcg cagggttata tcacgcaggt gataatgact tcctgttata tcgctgataa
     7981 taattttata tcttgagagt gttaataaca ggtaaatagt cttaattatc aaccaggaat
     8041 catcttagag cggatgattt gccaaactgc aaatcatccg tagagaaggg aaatggttaa
     8101 atgtcaacga cgtgaacggt gagtttactg tgctcaagat gcatcagatc gctggcgcgt
     8161 attttcgaat cggtaatcac gcgattgaat cgctcaacgg gaccgatgga gtatgaatgt
     8221 acagcaccaa atttcgagct gtcagtcagg actatcgctt cgcactcttt ttccagcacg
     8281 gcattgacca catcggtacg catcatatcg cgaccggtga atccagtttc aggttgccag
     8341 ccatcaatac caataaatgc tttgctgaaa tgtacctgct ggatgcactg gcgtgtcaaa
     8401 gggccaacca tactttcgct ttttttctgg tacacgccac cgagcaaaat gacttcacaa
     8461 ggcgcgtctt tcagcaaatg cgcaatgtag ctgctgactg tgatgatagt gacatttttc
     8521 ttctgttcgc ccagagtccg ggcgagcagg gcattgctgc taccattttc gataaagatg
     8581 gtttcgcccg gttgaaccag tgacgcggca aactcggcga gttcacgctt cagcgtatag
     8641 ttgctcatca tacgggtttc gacgtcatca ctatcaagcg aaactgcaaa gccatgtgca
     8701 cggcggaggt aactcagttt ttcgagggtg ttgagatcct ggcgaatggt aacttcagaa
     8761 actccagtgg cttttgccag atcggttacg ctaacctgac cctggtcaat gaccatctgt
     8821 agaatagttt gttgtcggga attcatcgct gtaattctta taacgttata atactaaata
     8881 aaaaaatact gacgcgccgg gcatcctcaa ataggaaaac ctgaaggcac gattgttaaa
     8941 tctcccacgg tgccttcggg atgtctggcg tagattcagc aacctggcgc aagagttctg
     9001 ctttaagtat ctcgagatta tggatcgcag agtggtaatc gcctgccaca aggatatcta
     9061 acacaatatc aatacgttcg gcaacgcgct gggcatcaaa ttcagacatc aaggacatcc
     9121 ttatagcaaa agggggaaaa tgaataatgc aaaaattgcc gctaaaagag aagtgtttaa
     9181 cagcaacggc taattatcat ccaggaatac gatatataat gacgggatat agcgctaagt
     9241 atatatattc atctacttat gcgcgcttca gatagcgttt ataccagcgt tcgaaggcga
     9301 cggcgggcat cggtttggca aacaaaaatc cttgccgctc attgatcccg ttcttggtta
     9361 aaaaagcatc ttcctttgca ctctctacac cttcggcgat cacctgaaga ttcaatgcct
     9421 gggccacagc gacgatcgcc cggaccagtg actgcgagac aggttgtttg tgaatatctc
     9481 gaacaaaaac ctggtcaagt ttgatggcat cgatcggaaa gcgcgccagt tgcgaaagtg
     9541 aagagtagcc ggtaccaaaa tcatccagat gcacttgcgc acctagttgg ctaaattgtt
     9601 gaataacaga cagtgccagt tcatcattct caatcagaca actctctgtc agttcaacat
     9661 ctatagggca gtattcaaaa ttgagttcct ggagaacctg tttcagggcg gtgaaaatgg
     9721 tttgatcggc gagctgacgt gcagaaatat ttaccgccac tcgcaggttt atgcctttat
     9781 cccgccactt tgccacctgg cgtacgacat cgagaatcac ccaacggcct aaaggcacaa
     9841 ttagccctga ctcttcggcg taggaaatga agtccaacgg tggaatcaac ccacgttcag
     9901 gtgactgcca acgtactagt gcttccagac tgcgcacttc gccacgccag gtgattttcg
     9961 gttgatagtg aataaccaac tgatcgtttt ccagtgcttt acgcaagttg gtatccagcc
    10021 agagatattc aaatacccgt tgattcattt ctggggtaaa aacgcaaaat tgtcctcgtc
    10081 cgccttcctt cgctgtgtac attgctgtgt cggcgtgacg aataatagcc gtgctgtctg
    10141 aaccatgttc gggagagagt gcaataccta ctgaacagct ggtataaact tcaattaaac
    10201 caatgcgaaa ggggagccgt aagcgggtca aaattcgtga tgccattgct tccagcgcgc
    10261 tttgtgaggt gttggatgcc agtaccagaa actcatcccc acctggacgc gccaacacct
    10321 ggtcatgttc gagacagctt aaaatagcca atgacacgtc gcgtaataac tggtcaccaa
    10381 acaaatgccc ataggcgtcg ttgacctttt tgaaattatc caaatcaaga tacacaaccc
    10441 caactttatt gttatctgca tgattaatag cgtgatcgat taaatcctgc attgcgttac
    10501 gattcggcag tccggtgata ctgtcggtat ttgccagaat acgcagtcgc tcctgagcgc
    10561 ggcgctcttc ggtaatgtcg gtgccggaac agattaaaaa aatctcgttt ttgccactgc
    10621 cgctgtggac aaatttattg cgaaacagaa acagccgctg gcctttacat gttggtatcc
    10681 acagttcgac ttcatatgca ttgccgcttc gaaaaaatac acggttattg cgcctggatg
    10741 ccgcagcttc acgacggctc ataaacagtt taaacacgct ttgcccaatg acgtcgtgtt
    10801 ctttcaaccc tgtgtaatct tcacataacc gattgaagcg ttggatattc ccccggctgt
    10861 cgagaatcac tattgcggaa tgtgcttcag aaactacctg ctcagcaaag gaaagcccgt
    10921 gtgataaatc acgagcaacc gccggtgtgt catgccatgc agacgcgttg ccagcccatt
    10981 cccgtttatt gatttttcgt cctaccagat gcacagaaag atcgctttca tcgacggtca
    11041 gactcatcat caggctggag gtgatgaccg tcatttccct gatgcgagca gcctgttcgg
    11101 cagataactg caatgtacga tcaggttctg tggtttcggt ggtagaaaag cgcaaaacat
    11161 cgctgctttc cgtcaaccgc cagtatggat tgtgcgatcc gagaaagttg tacaacgttg
    11221 tggactccct aacggttttc atgatgcata tcctccggtt aacagcggag tccgcggtta
    11281 aataaaagga acaacatgac ttacatgaa
//
LOCUS       DAPGWD010000131        11187 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPGWD010000131 DAPGWD010000000
VERSION     DAPGWD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.132"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            64..396
                     /gene="hdeA"
                     /locus_tag="RHX88_003425"
     CDS             64..396
                     /gene="hdeA"
                     /locus_tag="RHX88_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDV2232080.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            513..839
                     /gene="hdeB"
                     /locus_tag="RHX88_003426"
     CDS             513..839
                     /gene="hdeB"
                     /locus_tag="RHX88_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDV2232081.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            903..1550
                     /locus_tag="RHX88_003427"
     CDS             903..1550
                     /locus_tag="RHX88_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDV2232082.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1602..2372)
                     /locus_tag="RHX88_003428"
     CDS             complement(1602..2372)
                     /locus_tag="RHX88_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HDV2232083.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYSDRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(2369..3325)
                     /gene="chuU"
                     /locus_tag="RHX88_003429"
     CDS             complement(2369..3325)
                     /gene="chuU"
                     /locus_tag="RHX88_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HDV2232084.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(3412..4035)
                     /gene="chuY"
                     /locus_tag="RHX88_003430"
     CDS             complement(3412..4035)
                     /gene="chuY"
                     /locus_tag="RHX88_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HDV2232085.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVKVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSDRAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRVQNQECHGFVHRADVSAHIHELANAPALNKRIYSLIEPDLKPA"
     gene            complement(4035..4529)
                     /gene="hutX"
                     /locus_tag="RHX88_003431"
     CDS             complement(4035..4529)
                     /gene="hutX"
                     /locus_tag="RHX88_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDV2232086.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(4542..5879)
                     /locus_tag="RHX88_003432"
     CDS             complement(4542..5879)
                     /locus_tag="RHX88_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDV2232087.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFDEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDGCLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDEAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPASVREKP"
     gene            complement(5899..6813)
                     /gene="shuT"
                     /locus_tag="RHX88_003433"
     CDS             complement(5899..6813)
                     /gene="shuT"
                     /locus_tag="RHX88_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HDV2232088.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGDALVTQINQRLERVQQNVAAQKAPVKAMFILSAGGSAPQVAGKGSVAGAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            7495..9477
                     /gene="chuA"
                     /locus_tag="RHX88_003434"
                     /gene_synonym="shuA"
     CDS             7495..9477
                     /gene="chuA"
                     /locus_tag="RHX88_003434"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HDV2232089.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     VQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYISYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            9526..10554
                     /gene="chuS"
                     /locus_tag="RHX88_003435"
     CDS             9526..10554
                     /gene="chuS"
                     /locus_tag="RHX88_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HDV2232090.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIASLMNISEAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     MPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWHKQIAS
                     LIPEGVAA"
     gene            complement(10615..11145)
                     /locus_tag="RHX88_003436"
     CDS             complement(10615..11145)
                     /locus_tag="RHX88_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2232091.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT         2900 a         2640 c         2796 g         2851 t
ORIGIN      
        1 tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt tgaggatatt
       61 acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc agttgtgagc
      121 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
      181 gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt tgctgaagcg
      241 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
      301 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
      361 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
      421 ttccttgcat attgaacagg ccggaatatc ttgctttaaa agcagctatt cctcctgttc
      481 atatataatc tctatattga atgggttaca aaatgaatat ttcatctctc cgtaaagcgt
      541 ttatttttat gggcgctgta gcggctttgt cactagtgaa cgcacaatct gcgctggcag
      601 ccaatgaatc cgctaaagat atgacctgcc aggaatttat tgatctgaat ccaaaagcaa
      661 tgaccccggt tgcatggtgg atgctgcatg aagaaacagt atataaaggt ggcgataccg
      721 ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt gatcgaatac tgcaagaaaa
      781 acccgcagaa aaatttgtat accttcaaaa atcaagcatc taatgacttg ccgaattaat
      841 gaggtacaag taaaaaggag tagcaagttg agccatcttg ctgctccttt ttgcattttt
      901 atatgacagc agaatttatt atacgtctta tactcgcggc aattgcctgt ggcgctattg
      961 gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg cacacatgta ttaattggca
     1021 tgggaagcgc cctgtttatg attgtttcga aatatggttt tgctgacgta ctgtctttag
     1081 atcacgtcgg actggacccc agccgtattg ctgctcaggt ggtgacgggc gtcgggttta
     1141 tcggtgcagg taacattctc gttcgtaacc aaaatattgt aggtttgacg acggcagcgg
     1201 atatctgggt gaccgccgcc ataggtatgg ttattggcag tggtatgtac gaactgggta
     1261 tttatggttc agtgatgacc ttgctggtgc tggaagtctt ccatcaatta accttccgcc
     1321 tgatgaacaa aaattaccat ttacagctga cattagtgaa cggtaatacg gtatcaatgc
     1381 tcgactggtt taagcagcaa aaaataaaaa ctgatttagt ttcgttgcag gaaaatgaag
     1441 atcatgaagt ggtcgccatt gatatccagc ttcatgccac aacgtcgata gaagatctgt
     1501 tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc aattagctaa tctgtatagt
     1561 tatttattcg caggagtacc agtgcggtac tcctgttgct tttaatgatc gaggacgatc
     1621 agtggtgaat ggttggcagg atctttaagt acggtaatat ccgcgccgta gagcattgtc
     1681 agcgcctgct gtgtcaatac gtcctgcggt ttaccatttg cgataacctt tcctttttgc
     1741 attaatacaa tacggtctga ataacgcgcg gcaagattta gatcgtgcaa tacgcagcaa
     1801 acattgaact gccgttcgtg aactaactgg cgtaataaac ggaacaagtg ctgctgatgg
     1861 tgaatatcca gcgccgaagt tggttcatca agaaataacc atttcggtga gggagtaggt
     1921 tcccagagtt gcaccagcag gcgcgctaat tgcacgcgct gttgttcacc accggataat
     1981 tgccgatagt cgcggtttgc taacgcctga cagtcacata acgccattat ttgtgctgtt
     2041 tcattatcct gattaccagt acgatgcgga tgccgaccca tctggataac ttcttgtacg
     2101 ctgaatggaa acgccatgtg gctattttga cgcatcacgg cccggtgttt cgccagttca
     2161 gtaatcgacc attcatttaa tggcttatta aacaaccgac attcgccgct atcaggttga
     2221 aggtagccgg ttaactggcg aagcagcgta gattttcccg caccattagg gccgagaatg
     2281 gcaacaattt ccccaccggg aaaagtcagc gagacattat cagtcagacg ccgcccctga
     2341 aggctataaa ccagattctg ggcgctgatc atgtgatcct ccgatttcgc agaatcaacc
     2401 acataaaata agggccacca agcaggctgg ttaataatcc cacgggcatt tctgctggct
     2461 gtaccagcgt gcgggcaagc gtgtctgcca tcagcaataa acaggctccg gcgagggcgg
     2521 aacaagggat tagccagcgg tgatttgctc cggtagtcat gcgaatcaga tgcgggatca
     2581 ccaggccaat aaagccgata atgccgctta ccgatacggc agcaccaacg agcagtgagc
     2641 tcactaacag taattgctgg cgtttacgct taacgttcac gccgaggtaa tgggcttctt
     2701 catcaccgag ctgcagtaaa ttcagcgttc cggcgagaca agttgttgcg ataatggccg
     2761 gtaggatgaa tgaactggca accaataatg tcggccattg cgcctgtcca agattgccca
     2821 tcatccacaa tgtgagttgt cgtagctgct gctcatcgcc gatatagctc aggatgccga
     2881 tcgctgcgcc gcacagggca ttgatggcga taccggcgag taataattgc atcatgctgc
     2941 catcgcgatg gcgctgcgtg atgagaaaaa tgatggtgca gactactaaa cttccggcaa
     3001 tggcaaacac catttgctca tagagcacca gaaccactgg gaaggagaag ggcagcacaa
     3061 tagcaacgcc aaccattaat gcagaaccgc tactgacgcc aagtaatcct gggtcagcca
     3121 ttgggttgcg aaaaagcccc tgcataatta cccctgcggt tgccaacgct gcacctacca
     3181 gcaccgccag caaaacccgt ggtagacgaa tattgagcca gatatcgcgg tattcctcat
     3241 caaaaagggc atcaaaactg atctttaacg ctccctgact ggcaccgaac agcaccaggg
     3301 caagcaataa tagtgatagc cccataaaga ccctggcgga tgaaaatgaa tccttgagca
     3361 cgcaaaactc cgttatgacg ttgcggcgcg gagagtctgc gccgcatggg attacgccgg
     3421 tttcaggtca ggttcaatca gactgtagat ccgcttgttg agtgctggcg cgtttgccag
     3481 ttcatgaatg tgtgcagaga catcggcacg gtgaacaaaa ccatgacact cctgattttg
     3541 tacccgttgt gctttgcctg ttgctgcgcc gtcaagcaga ccaccagggc ggagaatggc
     3601 gtagtctagc tggctggttt gcagccagct ttccgccagt gtcttttcgc gcaccgcctg
     3661 accaaacgcg gcttttgccc gatccgataa gaacggccaa ctatcgccgc atcccaaaga
     3721 agtcaccaga atcatgcgac tgatgcctgc cttttcggcc tcatcaatca ctgttctgtg
     3781 cgccagataa tcctgagctc ctcccattgt tgagatgata agcgcatccg ggccagctgc
     3841 gcggcaagca gccgcaacta cgctggcatc acaggcgtcg cctgtaaaaa cctttacgcc
     3901 ttgttgcgcc agtttcgtgg cggcatcggc atgacgaatg acagcaacga ccggacgttg
     3961 ttccgccagc gccagttcaa gcgttctggc acccacacct tttccgcctg cgccaaagag
     4021 tagccacggc gtcatcaggc gtgttccttt aacgatgccg ccagagtatg gaaggcgctg
     4081 acttgttcgc tcaggagctg acgatggtca tccctgccga ggaagatttt cagcatggcg
     4141 ctgccttctt tgttgaaaaa gagaatggac gcggtgtcca tgcccataaa tttacgttcg
     4201 attaaggcaa tatgcgtgca gttttctgct ttgatatgcc cggacatgcc gtgctttccg
     4261 cgtaggttga aatagccgtg gcgatggaaa cccgaaggca gttcgccgct aaattcgagg
     4321 attacatcgg cagtatgtac taacgtggtg acgttgcccc attcgcagac ggtatcccag
     4381 acagtatcga atttatcgcc tggcactacg gtagaggagg gtagatttct cactacctcc
     4441 agcagtgtgg tgttgtattg ttcggctacc acctccagcg taccgtccgg ctccgttttc
     4501 aaaaattctt gtaacgagac atggctcata aatcactcct gttatggttt ttcacgcaca
     4561 ctcgctggcg cattaagtac ctgaattagt tcattaagag actgcaaaat attgcttgcc
     4621 cagaaacgac cttcattagt cagccgcagg caagttgaag catcgcgact taagcctttt
     4681 tggtgccatt gagccagtaa cggcgcgagt ttttcggcat gtggtgtaag ttcatccagc
     4741 ggtacacgcg ctgtttcaac acctgactgc aaggtatgac gccattgcgc attacgttcg
     4801 gcgttacgca tgatcatcat cagcggtttt ttgcctgccg cgacggattc atgccaggtc
     4861 tgaagattgc gttcgttcat ccaggagtaa ccattaatcg aaccaccggc tccggaacca
     4921 aaggccagac aatcggcacc ttgttttatc agcaggttat agagattgcg ttcgcgtgtg
     4981 gtacggcccc agtggctgtt actaatacag cgccaaccgg cttcgtccat gaagtcacac
     5041 ccttgcagat aaagatcgcg acgttctgcc ggagagggca cggtagtacg cccattttcc
     5101 acggctttgc ccagcggtgt attgggcagg acattgagcg catagagatc gacgccatcg
     5161 agaccgatat cgcgggcaat agccagatct tctccccagg tttgcgcatc ctgacccggc
     5221 aggccaaaca gcaggtcaca gaccaccgca gcacggtcac gtttgaccag gctttccata
     5281 aacgcaatgg cggttgggcc atccgaggtg cgggccattt tcttgcggat tttgctgtta
     5341 aacgactgaa taccaattga gaagcggttt gctcctgcat caagacaacc atcgattcgc
     5401 tcagcgtcaa aattcagtac ccggccttcg atagtgattt cacaatcagg ggccagtggc
     5461 agcttttctc gtaatgtggt gatgattctg gccaaatcgt gtgccgaaag cgcggaaggc
     5521 gtaccgccac cgaaatagac cgcgtggatg ggggcagact gatgcaatac gctgtccgct
     5581 tccatttcta tttcacgaat cagggcgtcg gtataatgag cacacgcatc ttcatcaaaa
     5641 cggttctgat aaaaaccaca aaacgtgcag tgtgtcgcac aaaaaggaat gtgtaggtaa
     5701 accagtcgct tacgaggtga tgccgtttga ttgatcatct cttgccaggt ctgtgccagt
     5761 tgctctttgg caaccggaat ggctccacga aacggcatca tggcgcgcct gtctttaaat
     5821 ggctgatcac cgtctaaagc aaaatgtggc gtgagatcca gggtgttatt tgcgttcatt
     5881 cgtctgattt aaatccattt attgcggcca aagctgctgg tgtaaagact caacgacatc
     5941 agcaatacgc ggacccattc ccagaatcag gttttgatca acggtgataa tgcgctggtt
     6001 tttccatgcg gcagtgtggg taattccagc aatagaacgt agccgattaa tatcgccatc
     6061 gaccatttgt gaggttacga caatcacttc agggttagcc gcaatcagtg attctgcact
     6121 gtagcttttg tactgctggt gggtagcgac gttttctgct ccggcaagcg aaagaatggc
     6181 gcccgcgacg ctgcctttac cggcaacttg tggcgcactt ccgcctgcgg agagaataaa
     6241 catggcttta accggggctt tttgggctgc cacgttttgc tgtactcgct ccaggcgttg
     6301 gttgatctgt gtcaccagcg cgtccccttg ttcaggaacc tgtaacgttt ttgccagttg
     6361 gcgaatgttg gcgtacatct gctcaagggt ggcaggcaca cgcggcaggg taacgacgtt
     6421 gactttctgc gcccgtagtt ggtcgagcac aatttgcggt ccagcatcct gccaggtaat
     6481 tacgctatcc gggcgaagtg acaaaatgcc ttcgctgctg agttgtttcc agtagccaat
     6541 atgcggcagt ttggcggttt ctggtggata agatgtcgtt tcatcgacac caaccacgcg
     6601 ttcgccagcg cccatcgcgt agattagctc cgtcagcgat cctcctgcga ccacgatacg
     6661 ttcggcagcc gttacgctaa aagcccagct aagagccagt actgccgtaa acaatttttt
     6721 tcgttttact gcggtcttcg cggcagaggc cacggctgaa atacataacg tcagtgggga
     6781 aaaaagaaac ggcctggtga tgatccttgg cataatatga gaacccttat caaattgaga
     6841 atgatcatga ttatcatttg agttatatat gaaatacaac cgtcgattca atacgcaagg
     6901 cgttacaagc gtatttattt ttgattttta tcaagatgct tcgtcagcta acgttcttgt
     6961 taccgactaa atttcatcgc gggaaaaagc tgagaaccca gagatatcga ggcttgcaga
     7021 gcaaagagaa gaaaaaatta tcactgttaa cgacaaaatt tagtgatgag gcatgggggg
     7081 ataaccataa aaacaaggtg gtttacaggg cgcaggatag gtttcataac cccctgttta
     7141 ttgtgttaat ggtggttaaa aaaaacttga tcatgaaaaa ttgatcgata ataattctca
     7201 ttatcacgtt tgcatttaac aaaatacgac catatggata acaaaacaat agcatttgca
     7261 agggataacg cacagctctc ttcactcctg atggggcata tcgttttgcc tgacaaagag
     7321 ggcgtgcttt tgcgtaattg ccgagttatc aggcaatttc atgggatata aacgcctgta
     7381 aactcacccg gcgaatatta tcgctctgaa ttttaaataa atttattttt cgccagccac
     7441 cccggtatta acccaggcag ccagcatttt ttattttcct catggagaat cgctatgtca
     7501 cgtccgcaat ttacctcgtt gcgtttgagt ttgttggcct tggctgtttc tgccaccttg
     7561 ccaacgtttg cttttgctac tgaaaccatg accgttacgg caacggggaa tgcccgtagt
     7621 tccttcgaag cgcctatgat ggtcagcgtc atcgacactt ccgctcctga aaatcaaacg
     7681 gctacttcag ccaccgatct gctgcgtcat gttcctggaa ttactctgga tggtaccgga
     7741 cgaaccaacg gtcaggatgt aaatatgcgt ggctatgatc atcgcggcgt gctggttctt
     7801 gtcgatggtg ttcgccaggg aacggatacc ggacacctga atggcacttt tctcgatccg
     7861 gcgctgatca agcgtgttga gattgttcgc ggaccttcag cattactgta tggcagtggc
     7921 gcgctgggtg gagtgatctc ctacgatacg gtcgatgcaa aagatttatt gcaggaagga
     7981 caaagcagtg gttttcgtgt ctttggtact ggcggcacgg gggaccatag cctgggatta
     8041 ggcgcgagcg cgtttgggcg aactgaaaat ctggatggta ttgtggcctg gtccagtcgc
     8101 gatcggggtg atttacgcca gagcaatggt gaaaccgcgc cgaatgacga gtccattaat
     8161 aacatgctgg cgaaagggac ctggcaaatt gattcagccc agtctctgag cggtttagtg
     8221 cgttactaca acaacgacgc gcgtgaacca aaaaatccgc agaccgttga ggcttctgaa
     8281 agcagcaacc cgatggttga tcgttcaaca attcaacgcg atgtgcagct ttcttataaa
     8341 ctcgccccgc agggcaacga ctggttaaat gcagatgcaa aaatttattg gtcggaagtc
     8401 cgtattaatg cgcaaaacac ggggagttcc ggcgagtatc gtgaacagat aacaaaagga
     8461 gccaggctgg agaaccgttc cactctcttt gccgacagtt tcgcttctca cttactgaca
     8521 tatggcggtg agtattatcg tcaggaacaa catccgggcg gcgcgacgac gggcttcccg
     8581 caagcaaaaa tcgattttag ctccggctgg ctacaggatg agatcacctt acgcgatctg
     8641 ccgattaccc tgcttggcgg aacccgctat gacagttatc gcggtagcag tgacggttac
     8701 aaagatgttg atgccgacaa atggtcgtct cgtgcgggga tgactatcaa tccgactaac
     8761 tggctgatgt tatttggctc atatgcccag gcattccgcg ccccgacgat gggcgaaatg
     8821 tataacgatt ctaagcactt ctcgattggt cgcttctata ccaactattg ggtgccaaac
     8881 ccgaacttac gtccggaaac taacgaaact caggagtacg gttttgggct gcgttttgat
     8941 gacctgatgt tgtccaatga tgctctggaa tttaaagcca gctactttga taccaaagcg
     9001 aaggattaca tctccacgac cgtcgatttc gcggcggcga cgactatgtc gtataacgtc
     9061 ccgaacgcca aaatctgggg ctgggatgtg atgacgaaat ataccactga tctgtttagc
     9121 cttgatgtgg cctataaccg tacccgcggc aaagacaccg ataccggcga atacatctcc
     9181 agcattaacc cggataccgt taccagcact ctgaatattc cgatcgctca cagtggcttc
     9241 tctgttgggt gggttggtac gtttgccgat cgctcaacac atatcagcag cagttacagc
     9301 aaacaaccag gctatggcgt gaatgatttc tacatcagtt atcaaggaca acaggcgctc
     9361 aaaggtatga ccactacttt agtgttgggt aacgctttcg acaaagagta ctggtcgcca
     9421 caaggcatcc cacaggatgg tcgtaacgga aaaattttcg tgagttatca atggtaatca
     9481 tctgccccga tatttcgggg catttatctg gaaggaagag agacaatgaa ccactacaca
     9541 cgctggcttg agttaaaaga acaaaatccc ggaaagtacg cgcgtgacat cgcaagttta
     9601 atgaatatca gcgaagcaga actggcattt gcacgcgtca cgcacgatgc gtggcggatg
     9661 cacggcgata tccgtgaaat tctggcggcg ctcgaaagtg ttggcgaaac caaatgtatt
     9721 tgccgtaatg aatatgcagt ccatgagcaa gttggtgcgt tcacaaacca gcatttgaac
     9781 ggacatgccg gattgatcct caatccgcgc gcgctggatt tacgtctgtt tctcaatcaa
     9841 tgggccagtg ttttccacat caaagaaaac acggctcgtg gcgaacgcca gagtattcag
     9901 ttctttgatc atcagggtga tgcattacta aaagtttatg ccaccgacaa taccgatatg
     9961 gcggcatgga gtgagcttct ggcacggttt atcaccgatg agaatatgcc gcttgagtta
    10021 aaagccgttg atgcgccagt tgttcaaacg cgagccgatg ccactgtggt cgagcaagag
    10081 tggcgggcga tgaccgacgt tcatcagttt tttacgttgc taaagcgcca caatctgacg
    10141 cgccaacagg cgttcaatct ggtggcagac gatttggcct gcaaagtatc caacagtgcg
    10201 ttggcgcaaa ttcttgaatc tgcacagcag gatggtaatg aaatcatggt gtttgttggc
    10261 aaccgtggct gcgtacagat tttcactggt gtggtagaaa aagtggtgcc aatgaaaggt
    10321 tggctgaata ttttcaaccc gacgtttact cttcatctat tagaagagag cattgctgaa
    10381 gcctgggtta cccgtaaacc gaccagcgat ggctacgtaa ccagtctgga attgtttgcc
    10441 catgatggta cgcagatagc gcaactttat ggtcaacgta cagaaggcga acaggagcaa
    10501 gcgcaatggc ataagcaaat tgcttcgctg ataccggaag gcgttgctgc ataacgtata
    10561 attaacggga ggtctggcta tttattgctg ggcctcttta tattgatcaa tcgttcacac
    10621 cagataatca atatgctgat ggcgtaccag ctcattgatc cgcttaagat tgaggatcat
    10681 catgatattg tgtttatggc agtagaacgt ttttagcgat attttcagtt tctccagaat
    10741 atcttccgtt gtcatttgat tcgcaatgca gctcagaacc tgatgttgct tgcgcgaaag
    10801 tgacacagcc gcttcaggca cagagcagta actgccattg atgagtgcga caaaatcaat
    10861 gatagtgata gtccgcggaa caaaggtcac cggcactttc cccaggcagc gtttaatatt
    10921 aaagggggag aaaataatca catggacagg ttttaatcgt tcaatttgag tgagaaaatt
    10981 agaatgaaat acattattca ttaatgtatc aataatgacg aaggcattct gatgcaacat
    11041 tacgtcgatc tcttcttcgt tctgtatatg aacgacatta cctttactca gaatgttttt
    11101 catcgcggtg aagaacatcg tatccctggt aattataaga aacataaccg actcctggtg
    11161 cggacattaa ctttataatc agtttgc
//
LOCUS       DAPGWD010000132        11166 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPGWD010000132 DAPGWD010000000
VERSION     DAPGWD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.133"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            35..139
                     /locus_tag="RHX88_003437"
     CDS             35..139
                     /locus_tag="RHX88_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDV2232092.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            450..2648
                     /gene="speF"
                     /locus_tag="RHX88_003438"
     CDS             450..2648
                     /gene="speF"
                     /locus_tag="RHX88_003438"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HDV2232093.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2645..3964
                     /gene="potE"
                     /locus_tag="RHX88_003439"
     CDS             2645..3964
                     /gene="potE"
                     /locus_tag="RHX88_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDV2232094.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4020..5660)
                     /gene="pgm"
                     /locus_tag="RHX88_003440"
     CDS             complement(4020..5660)
                     /gene="pgm"
                     /locus_tag="RHX88_003440"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDV2232095.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5686..6231)
                     /gene="seqA"
                     /locus_tag="RHX88_003441"
     CDS             complement(5686..6231)
                     /gene="seqA"
                     /locus_tag="RHX88_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDV2232096.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6416..7180
                     /gene="ybfF"
                     /locus_tag="RHX88_003442"
     CDS             6416..7180
                     /gene="ybfF"
                     /locus_tag="RHX88_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV2232097.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7320..7613
                     /gene="ybfE"
                     /locus_tag="RHX88_003443"
     CDS             7320..7613
                     /gene="ybfE"
                     /locus_tag="RHX88_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDV2232098.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7753..8283
                     /gene="fldA"
                     /locus_tag="RHX88_003444"
     CDS             7753..8283
                     /gene="fldA"
                     /locus_tag="RHX88_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDV2232099.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8493..8579
                     /gene="uof"
                     /locus_tag="RHX88_003445"
     CDS             8493..8579
                     /gene="uof"
                     /locus_tag="RHX88_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDV2232100.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8572..9018
                     /gene="fur"
                     /locus_tag="RHX88_003446"
     CDS             8572..9018
                     /gene="fur"
                     /locus_tag="RHX88_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDV2232101.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9102..9428)
                     /locus_tag="RHX88_003447"
     CDS             complement(9102..9428)
                     /locus_tag="RHX88_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232102.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9478..10884)
                     /gene="chiP"
                     /locus_tag="RHX88_003448"
     CDS             complement(9478..10884)
                     /gene="chiP"
                     /locus_tag="RHX88_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDV2232103.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2708 a         2753 c         2790 g         2915 t
ORIGIN      
        1 taccttcaac tgacacagca agaggtaaag gtaaatggaa aataataacc gcttaatgcc
       61 tcatataagg cggacaaccc atatcatgaa gtttgcccat cgtaatagtt ttgactttca
      121 tttctttaat gcccgctagt cttctgacta aagggcaccc caacgtacag gtctccctga
      181 ctttaagcat tacaggttaa tacctgtatt cctcggtgct catatactgc taaccctttt
      241 aaactctaaa taattcgagt cgcagcactt gcaacttgag gtatgacgag tatagccagt
      301 taccgggctg gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt atttatcagc
      361 ggtggagctt tgcttttttt ccggcgtgat cgatttctcc tttgagaaat tgaggacctg
      421 ctattacctg aaataaagag atgaacaaaa tgtcagaatt aaaaattgcc gttagtcgtt
      481 cttgcccgga ttgtttttcc actcatcgtg catgcgtgaa tatagacgaa agtaattata
      541 ttgacgttgc cgccattatt ttatcagtca gtgatgttga acgtggaaaa ctcgatgaaa
      601 tagacgctac tggctatgac attcctgttt ttattgcaac ggaaaatgaa gaacgtgtac
      661 ccgcagaata tctgccacgt atttctggtg tcttcgaaca ttgcgaatca cgtaaagagt
      721 tttatggacg tcagttagaa accgctgcca gccattatga aacgcaactg cgcccgccct
      781 tcttccgcgc actggtcgat tatgttaatc agggcaatag tgcttttgac tgccctggtc
      841 atcagggggg tgagtttttc cgtcgccatc cggcaggcaa tcagttcgtg gaatactttg
      901 gcgaaatgct cttccgctct gacttgtgta atgctgacgt agcaatgggc gatctgctga
      961 ttcatgaagg tgcgccatgt attgcacaac agcatgcggc taaagtcttt aatgccgata
     1021 aaacctattt cgttttaaac ggtacttcat cttctaataa agtggtctta aacgcattgc
     1081 tgacgccggg cgatctggtg ttgtttgacc gcaataacca taaatctaac caccacggtg
     1141 cgttgctaca ggctggcgcg acaccggttt atctggaaac cgcacgtaac ccgtatggct
     1201 ttatcggtgg cattgatgcg cactgttttg aagaaagtta cctgcgtgag ctgatcgcgg
     1261 aagtcgcacc gcagcgggca aaagaggctc gtcctttccg cctcgctgtg attcagttag
     1321 gcacctacga cggtacgatt tataacgccc gccaggtggt ggataaaatt ggtcatctgt
     1381 gtgactacat tctgtttgac tccgcatggg ttggctatga gcaatttatc ccgatgatgg
     1441 ctgactgttc gccgctgttg ctggatctta atgagaacga tccgggtatt ctggttacgc
     1501 aatccgtaca taaacagcag gctggttttt ctcaaacttc acaaatccat aaaaaagaca
     1561 gccacatcaa agggcaacag cgttatgtac cgcacaaacg catgaacaac gcctttatga
     1621 tgcatgcctc caccagcccg ttctatccgc tgtttgccgc actggatatc aacgccaaaa
     1681 tgcatgaagg tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat ggcattaatg
     1741 cccgcaagct gatcctcgat aactgtcagc atattcgtcc gttcgtacct gaactggtgg
     1801 atggtaaacc ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg cgcttcttcc
     1861 agtttgtacc aggtgagcac tggcactctt ttgaaggcta tgcagagaat caatactttg
     1921 tcgatccttg caaactgttg ctgacaaccc caggtattga tgcacgtaac ggcgaatatg
     1981 aagcgttcgg tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat ggcgtagtgc
     2041 cggaaaaatg cgatcttaac tccatcctct tcctgctgac tccggcagaa gatatggcca
     2101 agcttcagca acttgttgcc ctgctggtac gcttcgaaaa actgcttgag tccgacgcgc
     2161 cattagcaga agtgctgcct tccatctaca aacagcatga agagcgctac gccggttata
     2221 ccctgcgtca gttgtgtcaa gaaatgcatg atttgtatgc ccgtcacaac gtgaaacaac
     2281 tgcaaaaaga gatgttccgt aaggaacact tcccacgcgt aagcatgaat ccgcaagaag
     2341 ccaactacgc ctatttacgc ggtgaagtgg agctggttcg tctgccggat gcagaaggcc
     2401 gtatcgctgc cgaaggtgcg cttccttatc ctccgggtgt gctgtgcgtt gttccgggtg
     2461 aaatctgggg tggtgctgtt ctgcgttact tcagcgctct ggaagaaggg atcaacctgt
     2521 tgccaggttt tgcaccggag cttcagggtg tctatatcga agaacatgat ggtcgtaagc
     2581 aagtttggtg ctatgtcatc aagcctcgtg atgcgcaaag caccctgttg aaaggggaaa
     2641 aattatgagt caggctaaat cgaacaaaat gggcgtcgtt cagttaacca tactgacgat
     2701 ggtcaacatg atgggctccg gtatcatcat gctgccgaca aagcttgccg aagttgggac
     2761 aatttcaatt atctcctggc tggtgacagc cgtaggctca atggcactgg catgggcatt
     2821 cgcaaaatgc ggtatgttca gccgtaaatc aggtggtatg ggcggttatg ccgagtatgc
     2881 tttcggtaag tccggcaatt ttatggcgaa ctacacctac ggtgtctcgc tgctgattgc
     2941 taacgtcgcg attgctattt cggcggttgg ttacggcacc gaactgctcg gcgcgagttt
     3001 gtcgccagtg cagattggtc ttgcgaccat cggagtgctg tggatttgta ccgtggctaa
     3061 ctttggtggt gcgcgcatta ccgggcaaat cagtagcatt actgtgtggg gggtcattat
     3121 tccggtggta ggcctgtgca ttatcggctg gttctggttt agcccgacgc tttacgttga
     3181 ttcctggaat ccgcatcatg caccgttctt cagtgcagta ggttcttcca tcgctatgac
     3241 gctgtgggct tttctcggtc tggagtctgc gtgtgcgaat actgatgtag tggaaaaccc
     3301 ggaacgtaat gtgccaatcg cggtactcgg cggtacgtta ggtgcggcgg tgatttatat
     3361 cgtctccacc aacgtgattg cagggattgt gcctaatatg gagctggcaa attcaacggc
     3421 accatttggc ctggccttcg cacagatgtt cacaccagaa gtgggtaaag tcattatggc
     3481 gctgatggtg atgtcctgct gcggttcgct gcttggctgg cagttcacca ttgcccaggt
     3541 gtttaaatct tcatctgatg aaggctactt ccctaaaatt ttctcccgtg taaccaaagt
     3601 ggatgcaccg gtgcagggaa tgttgaccat tgtgattatc cagagcgggt tggcgctgat
     3661 gacgattagc ccgtcgctaa acagtcagtt caacgtgctg gttaacctgg ccgtggtgac
     3721 caatatcatt ccgtatattc tgtcaatggc ggcattagtc attattcaga aggtcgctaa
     3781 tgtgcccccc tcaaaagcga aagttgcaaa ctttgttgct tttgttggcg cgatgtatag
     3841 cttttatgcg ctgtactcat ccggggaaga agccatgctg tacggttcca tcgtgacctt
     3901 ccttggttgg acactgtatg gtctggtatc accacgcttt gaactgaaaa ataaacacgg
     3961 ttaaaaaagc aaagtaaaaa agggcgatct tgcgaccgcc ctttttttat taaatgtgtt
     4021 tacgcgtttt tcagaacttc gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga
     4081 tgttcttcac cgaggaagct ttcgcagtag atcttatatg cgtcttccgt gcctgacgga
     4141 cgcgcggcga accagccgtt gtcagtcatc actttcagac cgccaataga agcaccgttg
     4201 cccggagcag cagtcaggcg cgcggtgatc gggtcacctg ccagggtgct ggcgctcacc
     4261 atttccggag acagcttaga cagcgccgct ttttgtgcgg aagtcgcagc tgcctgcaaa
     4321 cggttgtagc tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc ctgcgggttc
     4381 ttaccggtga cagcggtgat ttccgccgcc agcagacaca tgatgatgcc gtctttgtcg
     4441 gtggaccacg gcgtgccgtc gaaacgcagg aaggaagccc ctgcgctctc ttcgccgcca
     4501 aaaccgaagc tgccgtcgaa cagaccatca acaaaccatt tgaaacctac cggtacttct
     4561 accagcttac ggcccaagtc attgaccaca cggtcgatca tcgcagaaga aaccagcgtt
     4621 ttaccgacag caacatcttt gccccactgc ggacgatgct ggaacaggta gttgatcgcc
     4681 accgccaggt agtggttcgg attcatcaaa cctgccggag tgacgatacc gtgacggtca
     4741 taatccgggt cgttagcaaa cgccagatcg aacttatcac gcagtgccag caggcccgcc
     4801 atcgcacact cggaggagca gtccatacgg atcgcgccgt ctttatcaag gtgcataaag
     4861 cggaaggttt gatcgacctg atcgttaacg atggtcaggt tgaggttgta atactcgcca
     4921 atacgcttcc agtattcgat accggaaccg cccagcggat caacgccaag cgtcaggccc
     4981 gctttctgaa tcgccgccat atcaacgata tcggccagcc cttccacgaa cggctgcacc
     5041 agatcctgct ctttcacatg accggatgcc atcgcttcgt cgagggagat acgcttcacg
     5101 cctttcaggc catcggccag cagtgcgttg gcacgatctt ccaccacttt agtgacgttg
     5161 gtatcagccg ggccaccatt tggcggattg tacttgatac caccatcttc cggcgggtta
     5221 tgggacggtg taataacgat accgtctgcc agcgggccac cttttttatt gtgaaccagg
     5281 atggcattgg aaacggcagg cgtcggggtg aagccattgt tttcctgcac aataacatct
     5341 acgccgttcg ctgccagcac ttccagaacg gaaataaatg caggttcgga cagggcgtga
     5401 gtatctttac ccacatagca agggccagtg ataccgtttt tcgcacgttc ttcagcaatt
     5461 gcctgagcga tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg
     5521 tgaccggaag taccgaattt caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca
     5581 taatattgcg ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc aggttggcct
     5641 gcacgattgt ggattgccat tgctttgtcc tttgtctgca acgttttaga tagttccgca
     5701 aaccttctca atcaattccg ccgggaattg catcgactgc atgatgtgtt cgatcatgct
     5761 gcatttacgg ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg gcacatgttt
     5821 cggcttggtc tgattaccat ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg
     5881 tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag
     5941 tgtagacaac agcagcataa agcgattgac cgctcgcttt tgctctgcgt attcatccga
     6001 aagcagaagt tcacgcattg cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc
     6061 gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag gctgtgatgc
     6121 ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt
     6181 gtggctggca atatagctgt agagttcatc atcaacttca atcgttttca tcttaatcca
     6241 gtgcagtgtc ttagctgaat acaagtcgcc aggagtatat gaataaatcc cggcagcgga
     6301 tagcgtcaag cctggcttgc agaaaaactc ggaataaacc ttatttgttg cagccctgtg
     6361 gcacaatggt caacataata ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa
     6421 attgaatatc cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca tcgttcttgt
     6481 ccatggtctg tttggtagcc tcgacaacct tggcgtactg gctcgcgatc tggtaaacga
     6541 tcacaatatc atccaggttg atatgcgtaa ccacggtctt tcaccgagag atccggtaat
     6601 gaattacccg gcgatggcgc aggatcttgt tgatactttg gatgcactgc agatcgacaa
     6661 agcaacattt atcggtcact ccatgggcgg taaagcggta atggcactta ctgcactggc
     6721 ccccgatcgc atcgataaac tggtggcgat cgatatcgca ccggtcgact atcacgtacg
     6781 ccgtcatgat gagattttcg cggctatcaa cgcggtcagt gaatcggacg cacaaactcg
     6841 ccagcaagca gcagcaataa tgcgccagca tcttaatgaa gaaggggtga ttcagtttct
     6901 gttgaaatct tttgttgacg gggagtggcg ctttaacgtg ccggtattgt gggatcagta
     6961 tccgcatatt gtgggttggg agaaaatccc ggcatgggat caccctgccc tgtttatccc
     7021 tggcggcaat tctccgtatg ttagcgagca gtaccgtgat gatttactgg cacaatttcc
     7081 acaggcacgg gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga
     7141 tgcggtatta cgcgctatcc gtcgctatct caatgattaa cgcaactgat taaaaaccca
     7201 gcgtccgcgt cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg atgtattatg
     7261 gcgcgctatc catccgggca gaggcctggc tgattgtttc ccccgaagtc accaagatca
     7321 tggccaaaga acaaacggac cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg
     7381 gacgaccgaa aactaatccg ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc
     7441 agctaaaacg cgacaaagta cgtggcctta agcgtgtcga actgaagctg aacgcggaag
     7501 ctgtcgaggc gctgaacgag ctggcggagt cgcgcaatat gagccgtagc gaactgatcg
     7561 aagagatgtt aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca
     7621 ctttcatgta gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc
     7681 tgctatgatt gcctttatcc gtgggcaatt ttccaccccc atttcaataa gtttcaagag
     7741 gttatttcac tcatggctat cactggcatc tttttcggca gcgacaccgg taataccgaa
     7801 aatatcgcaa aaatgattca aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt
     7861 gcaaaaagca gcaaagaaga tctggaagct tatgacattc tgctgctggg catcccaacc
     7921 tggtattacg gcgaagcgca gtgtgactgg gatgacttct tcccgactct cgaagagatt
     7981 gatttcaacg gcaaactggt tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa
     8041 tatttctgcg atgcattggg caccatccgc gacatcattg aaccgcgcgg tgcaaccatc
     8101 gttggtcact ggccaactgc gggctatcat ttcgaagcat caaaaggtct ggcagatgac
     8161 gaccactttg tcggtctggc tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt
     8221 gtagaaaaat gggttaaaca gatttctgaa gagttgcatc tcgacgaaat tcttaatgcc
     8281 tgatgtgatg cggcgtagac tcatgtctac gccgtattaa tagataatgc caatcaaaat
     8341 aattgctaca aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg
     8401 gccttgccgt tgtaaatgta agctgtgcca cgtttttatt aacaatattt gccagggact
     8461 tgtggttttc atttaggcgt ggcaattcta taatgatacg cattatctca agagcaaatt
     8521 ctgtcacttc ttctaatgaa gtgaaccgct tagtaacagg acagattccg catgactgat
     8581 aacaataccg ccctaaagaa agctggcctg aaagtaacgc ttccacgttt aaaaatcctg
     8641 gaagttcttc aggagccgga caaccatcac gtcagtgcgg aagatttata caaacgtctg
     8701 atcgatatgg gtgaagaaat tggtctggct acggtatatc gcgtactgaa ccagtttgac
     8761 gacgctggta tcgtcacccg ccacaatttt gaaggcggta aatccgtatt tgaactgaca
     8821 cagcaacatc accacgatca cctgatttgc ctcgactgcg gcaaggttat cgaatttagt
     8881 gatgattcca tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat tcgcctgact
     8941 aaccacagtc tctatcttta cggtcactgt gccgaaggcg attgccgcga agatgagcac
     9001 gcgcacgaag gcaaataagc cagcctgaac gagaaaagcc aacctgcggg ttggcttttt
     9061 tatacaaggg aaataagaaa agcgagagtt acagctctca cttatttgtt attactgcgt
     9121 atttcctgcc agaccttatc acaatcggct ttcaccgcct gatcatttcc ggtttgcgtt
     9181 gcacgcagac actgctgata atccagtacg cgtacacttt cctggtcggc aaactgctga
     9241 tgttgtttct ctttcttcag cacgtttaac acgctctggc aggcttcaat tttttctggc
     9301 gaaccttgtg cggtgttgat acaggcgctg tatgcctctt tcagacgact gtcttcctgt
     9361 ggcgcggttg attgcgcaca agctaccagc cccgatgcca ttatggcgat gagaatcagt
     9421 tttttcatag caaattcctc aaacgtgcgg caggcacaaa acctgccgcg tcgggcatca
     9481 gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct ggaagatgtt
     9541 accgtaaccg ccgccccagc ttgggatgtc agagtggttg tcgtattcgg tgaagtgcag
     9601 tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta
     9661 ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc tctgccaggt
     9721 cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt
     9781 ccagtttttc aggtcataca tcgcaccgaa gaagaccgct ttttcgccgt tggcgttgaa
     9841 gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt aggttggagt
     9901 catacgttgc aggaagtatc cctgctgacc gtcagcctta acccaggtgc cttcgaggcg
     9961 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc
    10021 gtcataaagg tcgttgacgc tgcgatcgtc aactttatcg cgcgtaccat agaactggta
    10081 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    10141 atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat ttttgaagtc
    10201 gtatttcgcc ccaagggagt gcagataatc aactttggtg gttttatcgt tctgataaaa
    10261 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    10321 caacgcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct gataagtacc
    10381 tggcataaag ctccagtgcg gcgctaacag cgtttgacca gttggctgaa tgtaacctgc
    10441 cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc ttataccgct
    10501 tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    10561 cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag caatatcaag
    10621 gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc aggtggagtg
    10681 agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    10741 ccagtaatat ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    10801 cggcattgcc ataaagcccg acattgctgt aacaccggcg atagccagcg ccagcgtact
    10861 acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat tgctgcacca
    10921 aaggtgaaca gcgaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt ctcgctgact
    10981 acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa ccagaatgta
    11041 agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa gtttgcggtt
    11101 gaaaggcttg cagaagaata aaagcgtgaa tttccagcaa gatgaaagcg gttacatttt
    11161 atttaa
//
LOCUS       DAPGWD010000133        11160 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPGWD010000133 DAPGWD010000000
VERSION     DAPGWD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.134"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(27..623)
                     /gene="nrfG"
                     /locus_tag="RHX88_003449"
     CDS             complement(27..623)
                     /gene="nrfG"
                     /locus_tag="RHX88_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDV2232104.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(620..1003)
                     /gene="nrfF"
                     /locus_tag="RHX88_003450"
     CDS             complement(620..1003)
                     /gene="nrfF"
                     /locus_tag="RHX88_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDV2232105.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(996..2714)
                     /locus_tag="RHX88_003451"
     CDS             complement(996..2714)
                     /locus_tag="RHX88_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDV2232106.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(2734..3690)
                     /gene="nrfD"
                     /locus_tag="RHX88_003452"
     CDS             complement(2734..3690)
                     /gene="nrfD"
                     /locus_tag="RHX88_003452"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDV2232107.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(3687..4358)
                     /gene="nrfC"
                     /locus_tag="RHX88_003453"
     CDS             complement(3687..4358)
                     /gene="nrfC"
                     /locus_tag="RHX88_003453"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDV2232108.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(4355..4921)
                     /gene="nrfB"
                     /locus_tag="RHX88_003454"
     CDS             complement(4355..4921)
                     /gene="nrfB"
                     /locus_tag="RHX88_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDV2232109.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(4966..6402)
                     /gene="nrfA"
                     /locus_tag="RHX88_003455"
     CDS             complement(4966..6402)
                     /gene="nrfA"
                     /locus_tag="RHX88_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDV2232110.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6794..8752
                     /gene="acs"
                     /locus_tag="RHX88_003456"
     CDS             6794..8752
                     /gene="acs"
                     /locus_tag="RHX88_003456"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDV2232111.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            8952..9266
                     /locus_tag="RHX88_003457"
     CDS             8952..9266
                     /locus_tag="RHX88_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDV2232112.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            9263..10912
                     /gene="actP"
                     /locus_tag="RHX88_003458"
     CDS             9263..10912
                     /gene="actP"
                     /locus_tag="RHX88_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDV2232113.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         2592 a         3113 c         2940 g         2515 t
ORIGIN      
        1 ataacataat cttcatcacc agatgattaa tctgatctcc gctgcaacaa tttcgccata
       61 ttaatcgact cgaccagctg tgtccggttg atccggggtg agttgagatc catcactttt
      121 tgccatagtt cgatggcttg cgcgtaattc gcctgcataa acgcatcgga agccagcagc
      181 atcagagcgg tgatttcgtt agagtccagc gcgagggctt tgtcgatcat tgcgcgagtc
      241 tgggcggtca tatgctggct ggcctggtaa taaagcaccg tcgccagcgc cgcatacagt
      301 tcagcgttct caccacgtag ttgcaacgcc tgacggtacg ccagcagcga attgctgtaa
      361 tcgttttgcc acagatagta ctcgcccagt aacgcccatt gttcgctgtt ttgtggatta
      421 gcgcggattt tatcctgcaa tgcctgaagc tgcgcttccg gggtttgctg gctggcaaac
      481 tgatgtagcg gatcgcgctg acgctgatat tccgcatgta ccgcctgcca ttttggactt
      541 aacagataac tgccgacaca gagaaatacc atcaaaatcg tgagtgttgt cagcattttc
      601 accggtattt tgggctcttt catcgcttcg ccctcactcg ccagaggatc agtgccatca
      661 gcagtaacaa caccactggc agcgcccaca acaccagcgt ctgacccgtt aacggtgggt
      721 tatagcgaac aaaatctccg tagcgttcgg tcatccagcc aatgatttcg acttcgttct
      781 tcccctccgc caccatgctg taaacctgat ggcgcatact gactgccacc ggcgcgttgg
      841 attccagtaa gttttgattc tggcactgcg gacaacgtaa ctggctggca atatttaacg
      901 cctgttgctg ttgttgcgga ttggcgaatt gccaggtgtc tacgacctga gcgcgcgcaa
      961 aacaggtaaa taacagcagt agcgtgagaa gtcctttatt catcgcgctt cctcccccgc
     1021 catccgctta acaatgcgcc cgcaatcatc aacaatcctc ccccccagat ccagcgcaca
     1081 ccgctttgta catacaagcg aaaagcgtaa cgatccgctc cggttttttc acccatgacc
     1141 gcataccagt catggatgcc gttccagcga attgacggtt ccatcatttg ctgacgacgt
     1201 gcttcgtaaa aacgccgctc cggcatcagt tcaccaatgc gttgctgatg gtcaaacagt
     1261 gccactatcg ctttttcgct ggtgtaattg cctttggctt gcagatcgag gcgctcaaaa
     1321 cggaaggtgt atcctgccag cgtcacctgc tgacccggct gtaaattcag gctgatctcc
     1381 tgacggctga cgctggaaac cacgatccca gcggcaaata acagcacgcc cgcatgagct
     1441 accagcgccg gaagctgcac gcgtttgcca gagacaaacg tcgccagcac aatcaccacc
     1501 agcatcaaca ggccaaacgg taacgtcgcg cggttaaaat acggcgcgcc aacggagagg
     1561 cgtccccagc ccagcagacc gtaaatcatc ggataaagcg ttcccaccag cacgatcagt
     1621 agcactgcgc aaaacagcaa cagcgtagcg aggattaaca tttcccgcga taaccccgaa
     1681 aaacgcacca ccgggccacc gtcccgcgct cgccagccat acagagccag agacgcaagg
     1741 ctaatcagtg caaacaggct gaacaacggc acggcgcgga cgttatccag tgcgaacgca
     1801 tgaaccgaaa ccagaatgcc agaacggaca attaaggtac ccagtagcga cagcatcaga
     1861 gttactatcg ccagcaacag cgaccagtgg cggaaaatct cccgctggcg tgtcagggac
     1921 aaactgtgca gcagcgcagt ggcagaaagc cagggtaata aagaggcgtt ttccaccgga
     1981 tcccagaacc accagccgcc ccagcccagt tcgcaatagg cccaccagga accgaggatg
     2041 atccccgccg ttaatgcgct ccagccaggt agtgcccagc gccaacaaat tctggcgcag
     2101 gcaccatcaa actcgccgcg cagtaaactc gccagcgcca cgctcgccgc caccatcaaa
     2161 ccgccatagc caaggtaaag cagcggtgga tgaaagataa gaccgggatg ttgcagcatc
     2221 ggattgagat cgcggccttc gattgctggt ggaaatatcc gcacgaaggg atcggaccac
     2281 agcactacaa acagtagcag tgcggcgagc atgagagata aaacggctag cgtcagcgga
     2341 aatagcggat cggtttgctg ccgataatgc caggcaaaca gcgcgctcca ggcggaaagc
     2401 agcagcaccc aaagcagcag cgaaccttca tggccgcccc acaccgctgc cagcttgagt
     2461 tcccacgaca gcaggctgta gctatgttgg gcgacataaa tgaccgagaa atcgctgatg
     2521 agaaaacaat acgtcagtac gccaaaggcg agcaacagga gcgcgaactg cgccagaatg
     2581 ccgatgcaag tgagtcgcat catggcaggc cagcgcaacc gcactcccgc aaaggccgtc
     2641 aacggggtca acacgttgac cccgagactt aacaacaacg ccagaaaacc gacttcagga
     2701 aggaatacgt ccagaaacca cctcttttct ggcttacgcc accgttaact gcccggcgta
     2761 gagaatgaaa aagcgcagca tcaacacgcc gaccagactg gccccacacg ccgccagcac
     2821 ggcaggaatg ccggaactgc gattgaccca cggtttgagc aacattggca caatcagccc
     2881 cagcccggcg acaccaagcc agaaccacca cgtccagaaa ccgccaccta atgccgccac
     2941 cagcgcacgc actttaccgt catcgcccag cgccagaccg acaaaaaacg ccaccagcag
     3001 gaagatttca ccccatacca cggggatttc catacggtgt acaaactgcg cttccgtgga
     3061 atgcgggtta ctgcgttggc gtatcgccat ggcgatcagc gccaccgccg caccggacga
     3121 gatgccggag aagaggaaca gcaccggcag gatcgggtta ttgaggaacg gatacgattt
     3181 cagcgccgac agcagaaagc cggtataagc ccccaacaac accgccaaca ccagcatcaa
     3241 tgtttccagt ccgcgatgaa cgggcgtcag caggctcaga accttttgca cgatccccag
     3301 cttcggcaac cagcgttgtt gcagggcaag caaatcatgt tcaaagattt tcgccagcca
     3361 cagcaccagc accaccatgt agagctgaaa cagcatcacc cccatcgaca tcaccgaggt
     3421 aaaactgtag tggaacatca gcttccagaa ggtccacggt cttgtcaggt ggaagacgag
     3481 gatcaacaga ccgaggatca ccgcgcccgg cccgacaatc agcgtggtgc gcagcaacgt
     3541 actgtctgca ccgcccgcct gcgggtagaa gcgacgtaac agcacggcca gcgtcaccag
     3601 accggcagaa ataccaatca aaaacaggta gatggcaatc ggccagtccc acaccagcga
     3661 ttcaaaatga aatgcggaag tctgcgtcat tggctcacct ccccgtattt aaacggtacg
     3721 cggtacagct tcggtttggt tcccagcgcc agcttgtagc ggtaagtagg cttctggcgc
     3781 agcagttgcg aaatctcact gttaggatcg tccagattgc caaacgtcag cgccttcgtc
     3841 gggcaagcct caacgcacgc gggcagctta ccggcctgca aattggtttt acggcagaaa
     3901 tcgcatttgt ccgccgtctt cgtgaccgga tggataaagc gcacgcggta cggacaggcg
     3961 gcgatgcagt actgacaacc gacgcagaga tcaggattaa catcgacaat cccgctagcg
     4021 gcatcacgaa aagacgcacc ggtcgggcag acgtcaacgc acggcgcatg atcgcagtgc
     4081 tggcaagact tacggaagaa gcgatatttc acatcaggaa attcgccctg cggctcgcta
     4141 cgaataatcg tcaggcgcga gacgccttcc ggcaccttgt tcacttcccg acaagcatcc
     4201 atacaggcgg tacagccgat gcataacgac tcgtcatgca ccatgccgta acgcacccca
     4261 ttgatattca acgttttcgc caccacgcgc cctgcggtgc cgctgacggc tgccagcacg
     4321 ccgacgccgg tgagaaactg gcgacgagac caggtcatgg ctgctcctta agcaacggaa
     4381 cagacgccgg gttaaagttc ggattggtgc gctgatcgct gtggcaatcg acgcaaatct
     4441 taatccgtcc tttgtcactt aacgtctgca tcgtatcttg ctgtggatgc agggaatggc
     4501 agctggcgca cgccactttg gttacgtgga catcgtgcgg ccagaacgct ttttgcaact
     4561 gttccggcag atgacaggac atacagacgc tgttctgctc tccaaccttg tacatcggct
     4621 cgttaaagcg catcacatct ttcacccctt cgcggtgttg cggtgatggc tggccgtgac
     4681 agttggtgca ggtgaccggc agtttgttat tcgggttgat aacggaagca tgttttccat
     4741 gcataccttc ggtatctggc ttatgacagt ccagacaggc ggcatccggg ttacgctgct
     4801 gggtaacttc gtagcgatca tcagatgcct gcgccgcagg ggtagcggta attccgttca
     4861 ggctccacaa caggcccgac gccagcaccc cggcagttaa taacgaacgt aatacgctca
     4921 tattcactcc attgtgtttt ccgccccttg cgaggcggaa cggggttatt ggcttaacag
     4981 accgttttta cgtgcctgtt cttcccactg cgggatcacc gttttgatga agtcctgctt
     5041 ctcggccttg atttgttcca tgttcaggcc aatagcctgc tgggctttct ctttggttga
     5101 gatatccggg atctggattt catgggtgat gcctttggtc gccagcaggc gcgccagttt
     5161 ggtgcgtgca tccgccgctt tatccatcgc cgtaccgagc atccgtaaac cttcttccgg
     5221 tgcgtgcata tgaatgccgt gggaagcgat cgccagatcc cagcgccact gggcatgacg
     5281 gatatcgtcc tgaattggct tcatttcggc ttccgtcgcg cctgcatcca gcgctgcttt
     5341 cgcttcgaag tgagcgtgaa ccagttgatc ttcaaccttg attttcaggt cgttaatcga
     5401 ctgcttacgt tccgcgacca ctttttgcaa ggcagctttg tcctgggtat ggcagttcgc
     5461 acaagtctgg gcgaagttat caaacggatt accaatttta tggtcggtgt agagtttgcc
     5521 ttcggcgttc tgcacttttg gcatatggca gtcgatacag gtcacgttgt ttttaccgtg
     5581 aatgcccgct gtccaggttt catattccgg gtgctgcgct ttcagcattg gcgttttcga
     5641 cagggagtta gtccagtcag agaaggcaat tttgtcgtaa tactgctcca tattttcgac
     5701 tttcatgccg tcatcccacg ggaatttaac cgctttgttt ttgccgtcga agtaatactc
     5761 cacatggcac tgaccgcaaa ccatcgattg ctggtcgaaa cgtccggctt tctcaaaagg
     5821 tttaccaatg gcttccatcg cgcgagccgc atacggacgg gaaagggtta actccggttt
     5881 gcctttggcg aactctggtg aggcggtgtt atggcagtcg gcacaaccta agttgttgac
     5941 gatttccggg ccgccgcgcg cccacttacc gtggaagtag ccatcttcgc cgtctttctg
     6001 gatcagacgc gccacatccg ggcttttaca actccagcac gccatcggta gcggaccatc
     6061 ttcagcgttt ttcggcgcac cggtacgcag ggtttcacgc acatcggtca cagcaaaggc
     6121 atgtccacgc ggcttgttgt aatcgcgcga gaagggatac cccgcccaca ggatcaccag
     6181 ccgtggatct tccgccaggg cgtcaacacg ctctgactgt tccgaggtgg ctttccagga
     6241 gagatattga tcgggatgct gcggggcaaa ggtttcattc ttcgcttcca cagttacagg
     6301 ttttgcggga gctgccgttt gttcagcgtg aacagaagtg aaaaagaaaa aaggaatcaa
     6361 taagctgaag atacggcgtg cgtttatttt tatccttgtc ataggggctt catccgaatt
     6421 gcgccattgt tgcaatggcg gtttttattg ttttcacgac agtaaccgca cctacactgt
     6481 catgacattg ctcgccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
     6541 aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac
     6601 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttgctg accttcaaaa
     6661 attaccctgc cgtttatttg cacaattcta cttttgcgtg atctgtcgcc caaatactaa
     6721 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
     6781 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
     6841 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
     6901 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccttacc agaaggtgaa
     6961 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
     7021 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
     7081 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
     7141 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
     7201 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
     7261 cgcggtgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
     7321 tgattccaac tcacgactgg tgatcacttc cgacgaaggt gtgcgtgccg ggcgcagtat
     7381 tccgctgaag aaaaacgttg atgacgcgct gaaaaacccg aacgtcacca gcgtagagca
     7441 tgtggtggta ctgaagcgta ctggcgggaa aattgactgg caggaagggc gcgacctgtg
     7501 gtggcacgac ctggttgagc aagcgagcga tcagcaccag gcggaagaga tgaacgccga
     7561 agatccgctg tttattctct acacctccgg ttctaccggt aagccaaaag gtgtgctgca
     7621 tactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
     7681 tccgggtgat atctactggt gcaccgccga tgtgggctgg gtgaccggac acagttactt
     7741 gctgtacggc ccgctggcct gcggtgcgac cacgctgatg tttgaaggcg tacccaactg
     7801 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
     7861 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgatcg agggtaccga
     7921 ccgttcgtcg ctgcgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
     7981 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
     8041 cgaaactggc ggtttcatga tcacgccgct gcctggcgct accgagctga aagccggttc
     8101 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
     8161 ggaaggcgct accgaaggca gcctggtgat caccgactcc tggccgggtc aggcgcgtac
     8221 gctgtttggc gatcacgaac gttttgaaca gacctacttc tccaccttca aaaatatgta
     8281 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
     8341 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagattg agtcggcgct
     8401 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
     8461 tcaggcgatc tacgcctacg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
     8521 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
     8581 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
     8641 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
     8701 cgtagtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
     8761 tgccggatgc gagtcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
     8821 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
     8881 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
     8941 gagaatctgt gatgaatggc actatttatc agcggataga agacaatgcg catttcaggg
     9001 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
     9061 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
     9121 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
     9181 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
     9241 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
     9301 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
     9361 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
     9421 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
     9481 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
     9541 cgcgctggtg tttacctccg gctatgacgg cttaatttac tcgctgggct tcctggtggg
     9601 ctggccgatc attttgttcc tgattgccga acgtctgcgt aacctggggc gctacacctt
     9661 tgccgatgtg gcctcttacc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
     9721 ttctctggtg gtggtggcgc tttaccttat cgcccagatg gtgggcgcag gtaaactgat
     9781 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
     9841 gatgtacgtc ctgttcggcg gcatgctggc gaccacttgg gtacagatta ttaaagccgt
     9901 tctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
     9961 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    10021 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    10081 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    10141 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    10201 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    10261 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    10321 caacctgttc ctcggtttta tttcagcagt tgcttttgcc accattcttg cggtggttgc
    10381 gggtctgacg ttggcgggcg catcagcggt ttcgcatgac ctgtacgcta acgtcttcaa
    10441 aaaaggcgcg accgaacgtg aagaactgcg ggtatcgaaa atcaccgtgc tgattttggg
    10501 tgtgattgcc attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtggg
    10561 gctggcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ctatgtactg
    10621 gtcgaaactg accacgcgtg gcgcgatgat gggtggctgg ctggggctga ttaccgcagt
    10681 agtactgatg atcctcggcc cgacgatttg ggtacagatc cttggtcacg aaaaagccat
    10741 cttcccgtat gaatacccgg cgctgttctc tatcaccgtg gcattcctcg gcatctggtt
    10801 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    10861 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    10921 cttccccggt cgcctgaccg gggaatactc ttcctctcca gcatgcatca ccttttccca
    10981 aaatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    11041 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    11101 tggtttaaat aatatgtttt tctctctttg ccagattaac gataaccact ctctcacaag
//
LOCUS       DAPGWD010000134        11149 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPGWD010000134 DAPGWD010000000
VERSION     DAPGWD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.135"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            13..312
                     /locus_tag="RHX88_003459"
     CDS             13..312
                     /locus_tag="RHX88_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDV2232114.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIKNPEYFSTYMQEELKALV"
     gene            complement(432..611)
                     /locus_tag="RHX88_003460"
     CDS             complement(432..611)
                     /locus_tag="RHX88_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDV2232115.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            685..2046
                     /gene="pabB"
                     /locus_tag="RHX88_003461"
     CDS             685..2046
                     /gene="pabB"
                     /locus_tag="RHX88_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDV2232116.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2050..2628
                     /locus_tag="RHX88_003462"
     CDS             2050..2628
                     /locus_tag="RHX88_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDV2232117.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2812..4176
                     /gene="sdaA"
                     /locus_tag="RHX88_003463"
     CDS             2812..4176
                     /gene="sdaA"
                     /locus_tag="RHX88_003463"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV2232118.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4307..5905
                     /locus_tag="RHX88_003464"
     CDS             4307..5905
                     /locus_tag="RHX88_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV2232119.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5909..7465)
                     /gene="yoaE"
                     /locus_tag="RHX88_003465"
     CDS             complement(5909..7465)
                     /gene="yoaE"
                     /locus_tag="RHX88_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDV2232120.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            7928..8899
                     /gene="manX"
                     /locus_tag="RHX88_003466"
     CDS             7928..8899
                     /gene="manX"
                     /locus_tag="RHX88_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDV2232121.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8962..9762
                     /gene="manY"
                     /locus_tag="RHX88_003467"
     CDS             8962..9762
                     /gene="manY"
                     /locus_tag="RHX88_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDV2232122.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9775..10626
                     /gene="manZ"
                     /locus_tag="RHX88_003468"
     CDS             9775..10626
                     /gene="manZ"
                     /locus_tag="RHX88_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDV2232123.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10681..11139
                     /locus_tag="RHX88_003469"
     CDS             10681..11139
                     /locus_tag="RHX88_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDV2232124.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2720 a         2828 c         2830 g         2771 t
ORIGIN      
        1 aaggataaga atatgcctgc tgtaatagat aaagccctgg atttcattgg tgccatggat
       61 gtatcagcgc caacaccaag ttcgatgaat gaaagcacag cgaagggaat ctttaaatat
      121 ttaaaagaac tgggagtacc cgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa
      181 ggctggaacc ctgggttcac ggaaaaaatg gttggatggg caaaaaaaat ggagtctggt
      241 gaacgcactg taattaaaaa tcctgaatac ttttcaacat atatgcagga agaactaaaa
      301 gcactggtct gagcttaatt tatatcagca taaatgggtc aggacgcttt taatcacata
      361 taaaaagcgt cctttttctc acaatcaaat gatcatcggc ttctataacg aaatcgatac
      421 ggattagtgt gttactcgtc ttcatcctca aaacgcgcca cgattcgctc gccggagtgg
      481 ttggcacgca gctcttctgc caccagcgcg attgcctggc cgctgctcat cccttgtgcc
      541 atcagttcct ggatccgctc gacagctttt tgctgctgtt catgggtgag tgaaggtaaa
      601 cctgcaaaca ttgttaactc ctgctaaatt gttggcgcta attatttcat gctacccggc
      661 acatagccag tagagtcagg actgatgaag acgttatctc ccgctgtgat tactttaccc
      721 tggcgtcagg acgccgctga attttatttc tcccgcttaa gccacctgcc gtgggcgatg
      781 cttttacact ccggctatgc cgatcatccg tatagccgct ttgatattgt ggtcgccgat
      841 ccgatttgca ctttaaccac tttcggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc
      901 acaacgacca ctgatgaccc gctacaggtg ctccagcagg tgctggatcg cgcagacatt
      961 cgcccaacgc ataacgaaga tttgccattt cagggcggcg cactggggtt gtttggctac
     1021 gatctgggcc gccgttttga gtcactgcca gaaattgcgg aacaagatat cgttctgccg
     1081 gatatggcag tgggtatcta cgattgggcg ctcattgtcg accaccagcg tcatacagtt
     1141 tctttgctga gtcataatga tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc
     1201 tcgccgcagg aagatttcac gctcacttcc gactggcaat ccaatatgac ccgcgagcag
     1261 tacggcgaaa aatttcgcca ggtacaggaa tatctgcaca gcggtgattg ctatcaggtg
     1321 aatctcgccc agcgttttca tgcgacctat tctggcgatg aatggcaggc attccttcag
     1381 cttaatcagg ccaaccgcgc gccatttagc gcttttttac gtcttgaaca gggtgcaatt
     1441 ttaagccttt cgccagagcg gtttattctt tgtgataata gtgaaatcca gacccgcccg
     1501 attaaaggca cgctaccacg cctgcccgat cctcaggaag atagcaaaca agcagaaaaa
     1561 ctggcgaact cagcgaaaga tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat
     1621 gatatcggtc gtgttgccgt agccggttcg gtaaaagtac cagagctgtt cgtggtggaa
     1681 cccttccctg ccgtgcatca tctggtcagc actataacgg cgcgactacc agaacagtta
     1741 cacgccagcg atctgctgcg cgcagctttt cctggtggct caataaccgg ggctccgaaa
     1801 gtacgggcta tggaaattat cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc
     1861 agcattggct atttgagctt ttgcggcaac atggatacca gcattactat ccgcacgctg
     1921 actgccatta acggacaaat atactgctct gcgggcggtg gaattgtcgc cgatagccag
     1981 gaagaagcgg aatatcagga aacttttgat aaagttaata agatattacg ccaactggag
     2041 aagtaagacg tggaataccg tagcctgacg cttgatgatt ttttatctcg ctttcaactt
     2101 ttgcgcccgc aaattaaccg ggaaacccta aatcatcgtc aggctgccgt gttaatcccc
     2161 atcgtccgtc gaccgcaacc ggggttgttg ctgactcagc gttcaattca tctgcgtaaa
     2221 cacgctggac aagtggcatt ccctggaggt gcagtcgatg acacggacgc atcagttatc
     2281 gccgccgcgc tgcgcgaagc tgaagaagag gtcgctatac cgccttccgc cgttgaagtt
     2341 atcggcgtgc tgccgcccgt cgatagcgta actggctatc aggtaacccc agtggtcggc
     2401 attatcccgc ccgatctgcc gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa
     2461 atgccgctcg cccaggcatt acatctgggt cgttatcacc ctttagatat ctaccgccgt
     2521 ggcgattcac atcgggtatg gctgtcctgg tacgaacagt attttgtatg gggaatgacc
     2581 gcaggcataa ttcgtgagct ggcgctgcaa attggtgtga aaccctgact atacttatct
     2641 ttacatctac aaaacactac ttgagacaat catcgcaata ttagttaaat cgcggttttt
     2701 gattagttta attcatgtga atagttaagc cagtcgccgc gttccctctt acactatgcg
     2761 ctgttattag ttcgttactg gaagtccagt caccttgtca ggagtattat cgtgattagt
     2821 ctattcgaca tgtttaaggt ggggattggt ccctcatctt cccataccgt agggcctatg
     2881 aaggcgggta aacagttcgt cgatgatctg gtcgaaaaag gcttactgga tagcgttact
     2941 cgcgttgccg tggacgttta tggttcactg tcgctgacgg gtaaaggcca ccacaccgat
     3001 atcgccatta ttatgggtct tgcaggtaac gaacctgcca ccgtggatat cgacagtatt
     3061 cccggtttta ttcgcgacgt agaagagcgc gaacgtctgc tgctggcaca gggacggcat
     3121 gaagtggatt tcccgcgcga caacgggatg cgttttcata acggcaacct gccgctgcat
     3181 gaaaacggta tgcaaatcca cgcctataac ggcgatgaag tcgtctacag caaaacttat
     3241 tattccatcg gcggcggttt tatcgtcgat gaagaacact ttggtcagga tgctgccaac
     3301 gaagtgagcg tgccgtatcc gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa
     3361 accggctatt cgctgtctgg tctcgctatg cagaacgaac tggcgctgca cagcaagaaa
     3421 gagatcgacg agtatttcgc gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg
     3481 atgaacaccg aaggtgtact gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg
     3541 cgccggatgt tagtttccag cgataaactg tctaacgatc cgatgaatgt cattgactgg
     3601 gtaaacatgt ttgcgctggc agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact
     3661 gcgccaacca acggtgcctg cggtatcgtt ccggcagtgc tggcttacta tgaccacttt
     3721 attgaatcgg tcagcccgga catctatacc cgttacttta tggcagcggg cgcgattggt
     3781 gcattgtata aaatgaacgc ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg
     3841 ggtgttgcct gttcaatggc tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa
     3901 caggtttgcg tggcggcgga aattggcatg gaacacaacc ttggtttaac ctgcgacccg
     3961 gttgcagggc aggttcaggt gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg
     4021 attaacgccg cacggatggc tctgcgccgc accagtgcac cgcgcgtctc gctggataag
     4081 gtcatcgaaa cgatgtacga aaccggtaag gacatgaacg ccaaataccg cgaaacctca
     4141 cgcggtggtc tggcaatcaa agtccagtgt gactaatact tcttactcgc ccatctgcaa
     4201 cggatgggcg aatttatacc cgctttctcg tctgctgtaa tattccccac tacacttcca
     4261 ctgttgcgtc aggcgtttgt cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca
     4321 acggatcatt aaaacctatc gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt
     4381 tacgctcgct tcgaccctga gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg
     4441 ggtagtacaa ttcgccaatc acgctgtaga ggaattagat aaagtactgc ttcccctaca
     4501 ggcaggtagc gaagtcttgc ttccgctgat tggtctgccc tgctctgtcg cccatttgcc
     4561 attacgtaaa caggcggcaa aactccaaac tgtgcgatcc attggcctgg tgcaagacgg
     4621 cacactctat tgctccagca tttttggtta tcgcaatgtg cccgtcgtgg acattctggc
     4681 tgaacttcct gcaccgcaac cacttttacg cctgacgatc gaccgtgccc tgattaaagg
     4741 cagtccggtt ttgattcaat ggacgcctgc agcgggcagt agcaatgctg gggtcatgga
     4801 gatgattaac atcgacttac tgacggcaat gctccttgag ccacaactgc cgcaaatcag
     4861 tagcgccagc ctgacggtgg acaaacggca tttgctctat ggtaatgggc tggtagattc
     4921 ccttccgcaa cctgaagaca atgaaaacta tcaggtttct tcgcaacgct ttccttttac
     4981 cattaacgtt aatggtccgg gggctacggc gctggcatgg cactatcttc caacacaatt
     5041 accgctggcg gtgctgctaa gtttactggt gggctacatc gcctggctgg cgaccgctta
     5101 ccggatgagc ttttcccgcg aaatcaatct gggcctggcg caacatgagt tcgaattgtt
     5161 ctgtcagcct ttgcttaatg cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg
     5221 ctggaacaat ccgcgtcagg gctggatttc accggatgtg tttattccta tcgcggaaga
     5281 acatcattta attgtgccac tgacccgcta tgtgatggca gaaaccattc gtcagcgcca
     5341 tgttttcccg atgagtagtc agtttcatgt tggcattaac gtcgcaccca gccattttcg
     5401 ccgtggtgtg ctgataaaag atctcaatca gtactggttt agcgctcacc cgattcagca
     5461 actgatcctc gaaatcaccg aacgcgatgc cttactggat gttgattatc ggattgcccg
     5521 cgagctacat cgtaaaaacg tcaaactggc gattgatgac ttcggcaccg gcaacagttc
     5581 attttcctgg cttgaaacat tacgtcctga cgtgctgaaa attgataagt catttaccgc
     5641 agctataggt tctgacgcgg ttaactcgac ggtgaccgat atcatcatcg cgctggggca
     5701 aagactgaat attgaactgg tggcggaggg cgtggaaacg caagaacagg cgaagtattt
     5761 gcgccgtcat ggcgtgcata ttttgcaagg gtatttgtac gcacagccga tgccgctacg
     5821 tgattttccc aaatggctgg cgggcagcca accgccgccc gcccggcata atggacatat
     5881 cacgcccatt atgccgttac gttaatgctt actcatcttc atcgtgcgcc ggttgctctt
     5941 taacaatgcg aaccagatca acacgataat cattggcttc aatgatggtg atatgcagtg
     6001 gccctacatc aatcacatcg cccacacggg gaatgtgacc atttgccgag atcacgaggc
     6061 ccgcgaccgt cgcgatatca tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca
     6121 aggcatgcaa atctgtaccg ccttttacca gccagccgtc accatcagta atgatttccg
     6181 gcgtttcgtc agcgtccggg aattcacccg caatggcttc cagcacatcc agcggcgtga
     6241 ccagaccttg taccacacca aactcgttgg tcacgataac aaagctcccg cgagcacgac
     6301 gcagcacgcc caacaggttg atcggatcga gggtttccgg gacgataatc gccggagacg
     6361 ccgaagcaat cgccgccaca tcaacgccct cttccagcgc caccagcagt tctttagcac
     6421 gtacaatacc gatgatttca tccagttcac cgcgacatac cgggaacaga ctgtgcggtg
     6481 aagagagcag ttgctcgcgg atttcatcga ccccgagatt agcgtcaacc cagcttattt
     6541 caccgcgcgg cgtcatgatc ccgcgcagag aacgcgacgc cagcgtcagt acgccgttaa
     6601 tcatgtaacg ttcttcttcg gcaaatgcac cttccgggat cggcatcggc atcgggttat
     6661 cggcatcgtg ctgaacattg gcctgacgtt tcccgcccat caaacgcagg atggcatcgg
     6721 cagtacgcgc tcgcagcggc aaagtcgact ggtggcgaat aaagttgcga cgcgcaatct
     6781 ggttaaacac ttcgatgatg atcgagaagc caatcgcggc atacaggtaa cctttcggaa
     6841 tgtggaaacc gaaaccttct gccaccagac tcagaccaat cattaacagg aagctcagac
     6901 agagcaccac caccgtgggg tgctggttaa cgaatcgcgt cagcggtttg gatgccagca
     6961 acataaccgc catcgcaatc actaccgccg ccatcatcac cggcagatgg ttaaccatcc
     7021 ctactgcagt aattaccgca tccaacgaga agacggcgtc aaggatgaca atctgtgtga
     7081 cgaccaccca gaaactggcg tagcctttac cgtggccgga atcatgatcg cggttttcca
     7141 gccgttcatg cagttcggtt gttgctttga acagcaagaa tatccccccg aacaacataa
     7201 tcaggtcgcg tccggagaag gagaaatcca tgacggtaaa tagcggtttg gtcagcgtga
     7261 ccatccatga aatcagcgac agcagcccca gacgcataat cagcgccagt gataacccca
     7321 gcaaacgcgc tttatcgcgt tgttttggcg gcagtttgtc agcaagaatg gcgatgaaga
     7381 ccaggttatc gatacccagc acaatttcga gaacaacaag cgtgagtagc cccgcccaaa
     7441 ttgaggggtc cattaagaat tccatgacaa gctcctgctt aaggaatagc tattcgacgc
     7501 cagaaataat gcaggcgtaa cgacaaaatg caaacgaaag gtgcggcata gagagccaga
     7561 aaggtaggcg ttaaaaggcc tgatgctgaa atgacgtcgg tgacgatcca tactgcgggc
     7621 tactgcccta tactccatgg ttgttaaacg ggagttaaac atatcagaga cgcctctgat
     7681 ttggcaaaga tttaccttcc tttgcaaacg aatgtgacaa ggatatttta cctttcgaaa
     7741 tttctgctaa tcgaaagtta aattacggat cttcatcaca taaaataatt ttttcgatat
     7801 ctaaaataaa tcgcgaaacg caggggtttt tggttgtagc ccttatctga atcgattcga
     7861 ttgtggacga cgattcaaaa atacatctgg cacgttgagg tgttaacgat aataaaggag
     7921 gtagcaagtg accattgcta ttgttatagg cacacatggt tgggctgcag agcagttgct
     7981 taaaacggca gaaatgctgt taggcgagca ggaaaacgtc ggctggatcg atttcgttcc
     8041 aggtgaaaat gccgaaacgc tgattgaaaa gtacaacgct cagttggcaa aactcgacac
     8101 cactaaaggc gtgctgtttc tcgttgatac atggggaggc agcccgttca atgctgccag
     8161 ccgcattgtc gtcgacaaag agcattatga agtcattgca ggcgttaaca ttccaatgct
     8221 cgtggaaacg ttaatggctc gtgatgatga cccaagcttt gatgaactgg ttgcgctggc
     8281 agtagaaaca ggccgtgaag gcgtgaaagc actgaaagcc aaaccggttg aaaaagccgc
     8341 gccagcaccc gctgccgcag caccaaaagc ggctccaact ccggcaaaac caatggggcc
     8401 aaacgactac atggttattg gccttgcgcg tatcgacgac cgtctgattc acggtcaggt
     8461 cgccacccgc tggaccaaag aaaccaatgt ctcccgtatt attgttgtta gtgatgaagt
     8521 ggctgcggat accgttcgta agacactgct cacccaggtt gcacctccgg gcgtaacagc
     8581 acacgtagtt gatgttgcca aaatgattcg cgtctacaac aacccgaaat atgctggcga
     8641 acgcgtaatg ctgttattta ccaacccaac agatgtagag cgtctcgttg aaggcggcgt
     8701 gaaaatcacc tctgttaacg tcggtggtat ggcattccgt cagggtaaaa cccaggtgaa
     8761 taacgcggtt tcggttgatg aaaaagatat cgaggcgttc aagaaactga atgcgcgcgg
     8821 tattgagctg gaagtccgta aggtttccac cgatccgaaa ctgaaaatga tggatctgat
     8881 cagcaaaatc gataagtaac gtattgtgtt gattatcact cagttttcac acttaagtct
     8941 tacgtaaaca ggagaagtac aatggagatt accactcttc aaattgtgct ggtatttatc
     9001 gtagcctgta tcgcaggtat gggatcaatc ctcgatgaat ttcagtttca ccgtccgcta
     9061 atcgcgtgta ccctggtggg tatcgttctt ggggatatga aaaccggtat tattatcggt
     9121 ggtacgctgg aaatgatcgc gctgggctgg atgaacatcg gtgctgcagt tgcgcctgac
     9181 gccgctctgg cttctatcat ttctaccatt ctggttatcg caggtcatca gagcattggt
     9241 gcaggtatcg cactggcaat ccctctggcc gctgcgggcc aggtactgac catcatcgtt
     9301 cgtactatta ccgttgcttt ccagcacgct gcggataagg ctgctgataa cggcaacctg
     9361 acagcgattt cctggatcca cgtttcttct ctgttcctgc aagcaatgcg tgtggctatt
     9421 ccggccgtca tcgttgcgct gtctgttggt accagcgaag tacagaacat gctgaatgcg
     9481 attccggaag tggtgaccaa tggtctgaat atcgccggtg gcatgatcgt ggtggttggt
     9541 tatgcgatgg ttatcaacat gatgcgtgct ggctacctga tgccgttctt ctacctcggc
     9601 ttcgtaaccg cagcattcac caactttaac ctggttgctc tgggtgtgat tggtactgtt
     9661 atggcagtgc tctacatcca acttagcccg aaatacaacc gcgtagccgg tgcgcctgct
     9721 caggcagctg gtaacaacga tctcgataac gaactggact aacaggtgag cgaaatggtt
     9781 gatacaactc aaactaccac cgagaaaaaa ctcactcaaa gtgatattcg tggcgtcttc
     9841 ctgcgttcta acctcttcca gggttcatgg aacttcgaac gtatgcaggc actgggtttc
     9901 tgcttctcta tggtaccggc aattcgtcgc ctctaccctg agaacaacga agctcgtaaa
     9961 caagctattc gccgtcacct ggagttcttt aacacccagc cgttcgtggc tgcgccgatt
    10021 ctcggcgtaa ccctggcgct ggaagaacag cgtgctaatg gcgcagagat cgatgacggt
    10081 gctatcaacg gtatcaaagt cggtttgatg gggccgctgg ctggtgtagg cgacccgatc
    10141 ttctggggaa ccgtacgtcc ggtatttgca gcactgggtg ccggtatcgc gatgagcggc
    10201 agcctgttag gtccgctgct gttcttcatc ctgtttaacc tggtgcgtct ggcaacccgt
    10261 tactacggcg tagcgtatgg ttactccaaa ggtatcgata tcgttaaaga tatgggtggt
    10321 ggcttcctgc aaaaactgac ggaaggggcg tctatcctcg gcctgtttgt catgggggca
    10381 ttggttaaca agtggacaca tgtcaacatc ccgctggtgg tttcgaccat caccgggcag
    10441 gatggacaga cccgtgtcac caccgtgcaa accattcttg accagttaat gccaggcctg
    10501 gtaccactgc tgctgacctt tgcttgtatg tggctgctgc gcaaaaaagt taacccgctg
    10561 tggatcatcg ttggcttctt cgtcatcggt atcgctggtt acgcttgcgg cctgctggga
    10621 ctgtaagact gttgtacact accggggcct tttggccccg tttttttatc tggaggatta
    10681 atgacaatca cggacctggt actgattctt ttcatcgccg cactcctggc cttcgcgatc
    10741 tacgatcagt tcatcatgcc ccgccgtaac ggccccaccc tgctggcaat tcctttgctc
    10801 cggcgtggtc gcatcgatag cgttatcttc gtcggattga ttgtcattct tatctataac
    10861 aacgtcacga atcatggcgc gctaataaca acatggttat taagtgcact ggctctgatg
    10921 ggtttttata tattctggat ccgcgttccg aagatcatct ttaaacaaaa aggttttttc
    10981 ttcgccaatg tctggattga atatagccga atcaaagcga tgaacttgtc ggaagatggc
    11041 gtgttggtga tgcaattaga acagcgtcgg ctgttaatcc gcgttcgaaa tatcgacgat
    11101 ctggaaaaaa tttataagct tctcgtatca actcaataac ttatgaatt
//
LOCUS       DAPGWD010000135        11106 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPGWD010000135 DAPGWD010000000
VERSION     DAPGWD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.136"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..845
                     /locus_tag="RHX88_003470"
     CDS             <1..845
                     /locus_tag="RHX88_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDV2232125.1"
                     /translation="EQTKLTLVTMTMQDHATLKEIKDLAKNITQRYGTPDLCIEAMRK
                     KNLKTMLFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQHLRAPVPMTWAFMMREAG
                     FPAVRPIYELPLSEQVINEVREELCTLGSYIALNLDGSVQERTFSLSVAEKLIAKIQS
                     DNDMPIVIVHGPKGEDKARKLAGCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSIL
                     HMASAYNTPVVAIYADYKTRWPAMADISESVVVGQKIDHISLDEFGKALKSALERVCV
                     STYV"
     gene            complement(1225..9213)
                     /locus_tag="RHX88_003471"
     CDS             complement(1225..9213)
                     /locus_tag="RHX88_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDV2232126.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLATQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANTNTVPYTLGALESAQSVAERFGISLAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSEKNLTPSPRNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPGYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPALSQKDSSVSLSTQTLSADSHSTATLPFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQVLTTGAISGTLTLMPQLNGADAAKAPAV
                     VNIISVSSSRTHSSITIDKNHYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLNGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSTEAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQASQQVNFIGDQSTAALTLSVPSGDITVTNTAPQHMTATLQDKNGNPLK
                     DKEITFTVPNDVASRFSISNGGKGMTDSNGVAIASLTGTLAGTHMITARVANSNVSDA
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGIHTAEAILLNGNRDTKTVNIAPDASNALVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQNIAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAIVVLQTSKVEIIGNGVD
                     ETTLTATVKDPFDNVVKDLPVTFSTDPADTQLSQSTSNTNDSGVAEVTLKGTILGVHT
                     AEATLPNGNNDTKTVNIAPDASNARLTLNIPAHQVVTNNSDSVQLTATVKDPSNHPVA
                     GIAVNFTMPQDVAANFTLENNGITVTQANGEAHVTLKGKKAGTHTVTATLINNNASDS
                     QPVTFVADKTSAQVALQMSKDEITGNGVDSATLTATVKDQFDNEVNNLQVTFSSAFSG
                     LTLTPGESNTNESGIAQATLVGVAFGEQTVTVSLANNGASDHKTVHFIGDTAAAKIIG
                     LTPVPNSIIAGTPQNSTGSVITATVVDNNSFPVKGVTVSFTSSTNSAEMTNGGQAVTN
                     EQGKATVTYTNTRSSTESGGRPDTVEASLENGSSTLSTSINVNADASTAHITLLQALT
                     DTVSAGNTTDLYVVVNDNYGNGVPQHKVTISVSPSEGVTPSNNAIYTTNSYGHFYASF
                     TATKAGVYQVTATLDNGDSIQQTVTYVPNVANAEITLGASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVRANFTLSDGGKAVTDAEGKAKVTLKGTKAGAHTVTAA
                     MAGGKSEQLVVNFIADTLTAQVNLNVTENNFIANNIGMTILQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVDSYGVSDTRPVT
                     LLADASTAKLADFTASSSSLIASTTEGATLTASVTDAYDNPLEGIKVNFRGLSATLSN
                     TTAETDAQGKAEILVTSTIAGTKIVVANLANVPTDTIMQTLTVKADADSATITSLEMP
                     EGQVMVATPLAVKAHVDDQFGNPVSEQLVTFSAEPSFFNMVISQDTVSTNTQGIAEVT
                     MTPNRYGSYTVQASLANGSSYEKDLVVIDLKLTLTASSPLIGINDPLGSTLTVRLTNP
                     NGAPVNNELVTFSVTPEDATLSSGTMKTNPSGEAQVVLTSNKVGTYTVTASITSGVII
                     QAQTIVKVTGNQSTAHVVSLSADPSTITANDSDINALKATVEDGSGNQVEGVNVNFAL
                     KGGSATLGSLTAVTDQHGFATTTVKSARTGSVTVSAGTSTGATQTANITLVAGPADTS
                     QSVLMNNRPSLKGDFIDSAELQLVLHDISGNPINVSEGMEFVQSDTNVPYVQVSAIDY
                     SNNFSGEYKATVTGGGEGIAKLIPVLNGVHQADLSTTIQFTRAQSKKMSGTVSVNGVD
                     LPTDSFPSQGFTGAYYQLNNDNFSPGKTAADYEFSSSASWVDVDATGKVTFKNVGSSW
                     VTITATPKSGGPGYIYEIRVKSWWVNSGDSFMIYSLAENFCSSNGNMLPRASLLSQGR
                     SRGIGTLYSEWGDMGHYMNEADFYSDMYWSSSPANSNEQYEISLATGEQSVMAKLGFS
                     HVTCYKNI"
     gene            complement(9526..9601)
                     /locus_tag="RHX88_003472"
     tRNA            complement(9526..9601)
                     /locus_tag="RHX88_003472"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9566..9568),aa:Asn,seq:gtt)
     gene            complement(9702..10499)
                     /gene="mtfA"
                     /locus_tag="RHX88_003473"
     CDS             complement(9702..10499)
                     /gene="mtfA"
                     /locus_tag="RHX88_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDV2232127.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRTCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            10593..10682
                     /locus_tag="RHX88_003474"
     tRNA            10593..10682
                     /locus_tag="RHX88_003474"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10627..10629,aa:Ser,seq:cga)
BASE COUNT         2718 a         2638 c         2651 g         3099 t
ORIGIN      
        1 ttgaacagac taaattaaca ttagtaacaa tgactatgca ggatcacgca acattaaaag
       61 aaataaaaga tttagcgaag aacataacac agcgatacgg tacgccggat ctttgtatag
      121 aggcgatgcg taaaaagaac ctgaaaacga tgttatttat cagtcagttg aaagcaaaaa
      181 cgaattttca ggttgtcggt ttaaccatga agtgctattc ccctttgtgt aagaacgcgt
      241 cccggatgga tcaacatctc cgggctcccg tacccatgac atgggcgttt atgatgcgtg
      301 aagcgggttt tcctgcagtc aggccaatat atgaattacc attaagcgag caggtaataa
      361 atgaagtacg tgaggaattg tgcactttag gatcttacat tgcgcttaat ttagatggca
      421 gcgtgcagga acgcacattt tcgttatcag tagctgagaa actcatcgcc aaaattcaaa
      481 gtgataatga tatgccaata gttattgttc atggaccaaa aggggaagat aaagcccgga
      541 aattagcggg ttgttataat aatgtctacc ggctatcctt atcaccctcg attaaacgtt
      601 cagcagcaat cataaaagat gcttatatcg caataacccc tgatacctca atattacata
      661 tggcaagtgc ctataatacc cccgtcgttg caatttatgc tgattacaaa acgcggtggc
      721 ctgcgatggc agatatctcg gagtcagtgg tcgttgggca aaaaattgat catataagtt
      781 tggatgaatt cggaaaggcg ctaaaaagtg ctttggagag agtatgtgtc agtacttacg
      841 tatagagggt aatgccgatc tatgctgaac aggggacatt aataccctga ggttggtcaa
      901 tcaccacaaa aaaagccttt gaatcaaagc aatactcacc gaataataag gcaacctgaa
      961 cttaaatccc cgttaactgc catacattat tatgacttgc actgatgtaa ttagctaaac
     1021 attatcgtga tttgactaaa tagcaaaaat gatagctatt aaaaatagct catatacttt
     1081 gtttcccgct gccgtagaat agatggtcct tccatgtttc aagcatttat tcaaaaaaaa
     1141 aggggctcga ctgtcttacg ataaacagag agggattgta aagtcgagtg cccctaatgg
     1201 agcactctat aaaaaaatca acaatcaaat gtttttataa catgtcacat gagaaaatcc
     1261 aagttttgcc attacgcttt gctcacctgt tgccagggaa atttcgtatt gttcattgga
     1321 gtttgcggga ctagatgacc aatacatatc tgaataaaaa tcggcttcat tcatgtaatg
     1381 ccccatatct ccccattcac tgtacaatgt ccctatacct cgggaacgac cctggcttaa
     1441 aaggcttgca cggggaagca tgttgccatt gctactgcaa aaattttcag caaggctgta
     1501 tatcatgaaa gaatcgccgg agtttaccca ccaactcttc acccggattt cgtatatata
     1561 gccagggcct cctgattttg gcgtcgctgt aatcgttacc caactgcttc cgacattttt
     1621 aaatgtcact ttaccggtag cgtcaacatc gacccaggag gcagagcttg aaaactcata
     1681 atcagccgcc gttttgcccg gggaaaagtt gtcattattc aactgataat acgccccggt
     1741 aaatccctga gaagggaatg agtctgtcgg taggtcaaca ccattgactg acactgtacc
     1801 gctcattttt ttactttgtg cgcgagtgaa ttgtattgtg gtactcagat cagcttgatg
     1861 aacaccattc aacacgggga tcagctttgc aataccctct ccaccgcctg taacggtggc
     1921 tttgtactcg ccggagaaat tattactgta gtcaattgcg ctaacttgca catagggcac
     1981 gttggtatct gactgcacaa attccatccc ttcagaaaca ttgatcggat tgcctgatat
     2041 atcgtgcaga acaagttgca gttcagcact atcgataaag tcccctttca gtgatggccg
     2101 gttgttcata aggacggact gcgaggtgtc tgctgggcca gccaccaacg ttatattagc
     2161 tgtttgcgtt gcacctgtgc tcgttcctgc actaacagtt acgctcccag ttcgcgcact
     2221 tttcacggtg gttgtcgcaa atccgtgttg atcggtcacc gctgttaatg accctaatgt
     2281 ggcagagcca ccttttaatg cgaagttcac attgacgcct tcgacttggt taccactgcc
     2341 gtcctcaaca gtagccttca gcgcgttgat atcgctgtcg ttggcagtga tagttgacgg
     2401 atcagcgctc aggctgacca catgggctgt gctttggttg ccagtaactt tcacgattgt
     2461 ctgcgcctga ataattacgc cactagtgat agatgctgta accgtatacg taccgacttt
     2521 attgctggtc agaacgactt gtgcctcccc ggaaggattg gttttcattg ttccgctact
     2581 taacgttgca tcttctggcg ttacgctaaa ggtaacgagt tcattgttca ctggggcccc
     2641 atttggattg gttagcctta ctgtcagagt tgaacctaat gggtcattga tgccgataag
     2701 tggacttgat gctgtgagtg tcagttttaa atcgattacc accaggtcct tctcataaga
     2761 ggagccattc gccagggatg cttgcacagt atacgaaccg tatctattgg gcgtcatggt
     2821 gacctcggct ataccctgtg tattcgtaga gaccgtatcc tgactaatga ccatgttaaa
     2881 aaatgacggc tctgcactga atgtcacaag ctgttcagaa actggattgc caaactggtc
     2941 gtcgacgtgc gctttaaccg ccaggggggt tgcgaccata acctgacctt caggcatttc
     3001 cagactggtg atcgtcgcag aatcagcatc tgctttgaca gtcaacgttt gcatgatggt
     3061 atctgtaggt acatttgcca gattagccac aacaattttc gtccctgcaa tggtgcttgt
     3121 tacaagaatt tctgccttac cctgagcatc tgtttcagcg gtggtgttac tcagcgtggc
     3181 ggataagccg cggaaattga ccttgatgcc ttctaatgga ttgtcataag cgtcagtgac
     3241 acttgccgtc agggtcgcac cctcggttgt gctggcgatt aatgagcttg atgatgcggt
     3301 aaaatcagcc agtttagcgg tactcgcatc ggcaagcaaa gtcaccggcc tcgtatcact
     3361 gacaccataa ctgtccacgc tgaccgtcac gggataggtg ccggattttg taccgctcag
     3421 tgtaacttca gccttgccgt tgatgtcagt aatggcggaa ccgccttgtc cgagagtaaa
     3481 gcttgcactc acatctgccg gtagtgtgaa tgtcaccgcc tcattggcta acgggttgcc
     3541 gtttccatca gtcactgttg cctgaagtat ggtcattcca atgttattgg cgataaagtt
     3601 gttttcggta acgttaagat taacctgcgc agtgagcgta tccgcaataa agttcaccac
     3661 caattgctca ctcttaccgc cagccatcgc tgctgtcacg gtatgtgctc ctgcttttgt
     3721 acctttcagt gtgactttcg ctttgccttc agcatcagta accgctttac cgccatcgct
     3781 cagcgtgaag tttgccctca catcttccgg gagagtaaat gttacttcag tgttggctat
     3841 cgcattgccc tctgtatctg cgactgttgc tgttaatgtc gtgagatcgt tattgtcggc
     3901 aatcaccgga tcttttgagg ctcccagcgt gatttcagca ttcgccacgt tcggcacata
     3961 ggtcactgtt tgttgtatcg aatcgccatt gtcgagcgtt gccgtcactt gataaacccc
     4021 ggcttttgta gcggtaaagc ttgcgtaaaa atggccgtag gagttggtag tatatatagc
     4081 gttattactg ggggtcacac cttcacttgg cgaaacgctg atggtcacct tatgttgggg
     4141 tacaccgttt ccgtaattat cgtttaccac aacatacaga tcggtcgtgt tgcctgcgga
     4201 gactgtatca gtaagtgctt gtaacaaggt gatatgtgcc gtagacgcat cagcgttgac
     4261 attaattgat gtgctaagcg tggagctacc attttccaga ctggcctcaa cggtatccgg
     4321 cctccctcct gattctgtcg aggaacgggt attggtataa gtgacggttg ccttaccctg
     4381 ttcgttcgtt acggcttggc cgccattcgt catttcagca gagtttgtac tgctggtgaa
     4441 actcacagtt acacctttca ccggaaagct attattatca acgactgtcg cggtgatgac
     4501 gctgccggtg ctattctgcg gggtaccagc gattatgctg tttgggacag gcgtcaaccc
     4561 aataattttt gccgcagctg tgtcgccaat aaagtgcaca gttttgtggt cgctggcacc
     4621 attattagca agtgatacag tgaccgtctg ctcaccaaag gcaacgccta cgagagtggc
     4681 ctgcgcgatg ccagactcat tggtattact ctcccctggg gtgagggtga ggcctgaaaa
     4741 ggctgagctg aatgttacct gaagattgtt cacctcattg tcgaactggt ctttaaccgt
     4801 tgcagttaac gttgcactat cgacgccatt acccgtgatc tcatcttttg acatctgcag
     4861 ggcaacctga gccgaggttt tgtctgccac aaatgttacc ggctgcgaat cactggcatt
     4921 gttattaatc agcgtcgcgg taactgtatg cgtgcccgct tttttacctt tgagggtgac
     4981 atgcgcttcc ccattggcct gggtgacagt aataccgtta ttttcaaggg taaagtttgc
     5041 cgcgacgtct tgtggcatgg tgaaattcac cgctatcccc gccaccggat gattcgacgg
     5101 atctttcact gtagctgtca gctgcacact gtcgctgtta ttcgtcacca cctggtgagc
     5161 agggatgttc agcgtgagcc gtgcgttgct ggcatcggga gcaatattga ctgtcttcgt
     5221 atcgttgttg ccgttaggca gcgtggcttc ggctgtatga acacccaaaa tcgtgccctt
     5281 aagggtaact tcagcaacac cactgtcgtt agtgttgctc gtgctctgac taagttgagt
     5341 atcagccgga tcggtactga aggtgaccgg tagatctttt actacgttat caaaaggatc
     5401 tttcactgtt gctgtcagtg tcgtctcatc cacgccattc ccaatgattt ccactttcga
     5461 tgtttgcaga acgacaattg cgctgtcctt atccgccacg aatgtgactg gttgcgcatc
     5521 gctggcatta ttgttaccca gcgttgcggt aaccgtatgc gtgcccgctt ttttgccttt
     5581 gagggtgaca tgcgcttcgc cattggcctg ggtaatggca atgccgttat tttcaagggt
     5641 aaagtttgcc gcgatgttct gaggcatggt gaagttcacc gttattcccg ccaccggatg
     5701 attcgacgga tctttcaccg tcgccgtcag ctgcacgctg tcactgttat tcgtcaccac
     5761 ctgttgagca gggatattca gcgtgactag cgcgttgctg gcatcgggcg caatattgac
     5821 tgtcttcgta tctctgttgc cgttaagcag tatggcttcg gctgtatgaa tacccaaaac
     5881 cgtgccctta agggtaactt cagcaatacc gttctcatta gtattacgcg cgttcagact
     5941 cagttgcgtg tctgcgggag aggtgcggaa gactactgac agatttttaa ccacgttatc
     6001 aaaaggatct ttcactgttg ctgtcagcgt cgtctcatcc acgccattcc caatgatttc
     6061 cgctttcgat gtttgtagaa cgacaaccgc tctgtcttta tctgccacaa acgtcattgg
     6121 ctgagcatcg ctgacattgc tgttagccac acgagccgtg atcatatgcg tgcccgctaa
     6181 cgtgccggtc agggaggcga ttgcaacccc gttactatcc gtcatgcctt ttccgccgtt
     6241 gctaatcgag aaccgacttg cgacgtcgtt tggcacagtg aaggtgattt ctttatcttt
     6301 tagtgggttg ccatttttat cctgcaaggt tgcagtcata tgttgcggag ctgtgttggt
     6361 gacggtgata tcacctgaag gcacactgag ggtcagggca gcagtacttt gatcaccgat
     6421 aaaattcacc tgttgactag cctgggaacc agagctgaca gaggccgtaa ccgtataatc
     6481 accatttttc aaactggtca gcgtggctgt ggcgatcccg tcgtggctat tcacttcggt
     6541 ttggctcagt ttcgcctctg ttgaattaac attgaaggtg accttgacgt cattgagcag
     6601 gttgcctttt gcatcccgga ctgtcgcggt catggtggcg ctgtcattgc cgtcagcgac
     6661 cacttcattt ttcgacttct gtagatcaac ctgcgcggta ctcgagtcac cgacaaaact
     6721 gacgattaac gtttgtttca cgccattttc aagggtgact tcaaccgtgt tgtcttcctg
     6781 cttactactt ttcagatcaa aagtcgccag accattaaca tccgtttttg cggtgttttg
     6841 attgttgaag gaagttgccg atccgtttaa taccgcaaac gtgacggtat gatcattgat
     6901 tgggttgctt ccttcatcag cgacattgac cgagacggta tttgctgcat tctcattggc
     6961 gagcacacca ttattgctgg cagataatgt cgcaattttc gctgactgcg ggttggcgtc
     7021 gatgataaat ccagcggtat gcaaatcttc attccagttt tgcattaaca gcttcgcagt
     7081 aagtccactg cctttggtat aggcggtata ggttgcctta tagacgccat ctgcggtttc
     7141 tttccagtct gtagtgactc ctggtttcac gttgtcgatg ctgactgcag tattcagttg
     7201 ctgtttttgc tccttaacag gtttgtcatt ttcatctctc agttctaccg tcacctcgat
     7261 aggattgccg gacagataat ggtttttatc aatcgtaatt gacgagtgag ttcgggatga
     7321 cgaaacagaa atgatattca ccacggcggg ggctttggcc gcatccgcac cgttcagctg
     7381 tggcatcagc gtcagcgtgc cagagatcgc tcctgtggtc aggacttggg tatagcttcc
     7441 gtcaccatta tctttccagt cagaaagggt aatgtcctga acaccttcat gacgcgtcga
     7501 gagcaccagc ccgataacag gattaccagc tgcatcatga gctataaaag gcagtgtggc
     7561 ggttgaatgg gaatccgcac tcaatgtttg ggtacttaac gataccgagg aatctttctg
     7621 gcttagcgca ggtgcctgaa cgaccaccat gctttgttca cgattcgaca aattgccttt
     7681 gacgtcttca gcggtaactt caatcggcca ggtgttatcg gtttctggcg tactggtgaa
     7741 ccggtaacca ggcagggtaa ccagaatatc tttacccgtt gtgaccactt taccaccggc
     7801 agcttccaga gcggtggctt cgacgttata gcctttcagg gcatatttgg tttgtagcga
     7861 cgaaaccagt gatttcactt ctcctgactt ccctgtcacg gggtctgtca gggtcaggcg
     7921 aaccagttct tttttgcgat attccagaac gatgttgttg ttgcgatcca ccagatcata
     7981 acggctgcct gcaaggctac gccgtgcagc gacttcgttc gggtcaagct gtttctgcat
     8041 tgcgctgcca ggttgccagg taaaatcgac ggcaaaacgg gtgtcatttt cgccctgttt
     8101 accctggcgt tgctccgcgc tgaaggtcat cagcgggaag ggggtatagt taagtccagc
     8161 ggttatggca tgaggattac tttgccgatc gtctttatcg aacagggcca cttcatcgcc
     8221 ataatactgt tcatagacca gtttaccgcc aaggtgcggc caggcgggta accagccttc
     8281 tgcgcgtaca tcccagccat tggccgggcg tgcttcataa tcgttgtcca gttcaggtgc
     8341 gctgcgccag ttggtcagtc gcaaatagcc gttactgctt aatttaagat agtcgcgcca
     8401 gtactccgcg ccaatgccgg cgcgggagtg gtaacggcta agatcgtggt cgaaaaagaa
     8461 gttgatgccc gacatccatg tgggagtgaa atgacgccaa cccaagccgt tgttaatctg
     8521 cgtacgctcg tcagtacggt ggagagtatg ctggctgaaa aagagattat caggcgtttc
     8581 ataccatgga tggagaaaat cgaactggga attcttcagg ctaaaatctt catccacgcc
     8641 cagcgtgatt cttgcggtgc cgaagcggct taaccagtct gtcattgcgc cagatgcctg
     8701 agaagaggcc catccacgcg ccatattggc cgcttgctcg ctgttcatat cttcggcgag
     8761 cagagacccg atttgctgtg aagtactggc tatctgttgc tcaaggttgt cactgctatt
     8821 acgcggcgac ggggttaaat ttttttcact aacttgtgcc gggacatcca gttcatcacc
     8881 ctggcggaca ttatcaaaac ctcgagcaaa cgtacgaaac tggttgagtt tgcgtaactc
     8941 agccaacgaa ataccgaaac gttcggcaac gctttgggcc gattcgagcg ctccaagggt
     9001 gtagggcacc gtattggtat ttgcaatggc gatttgtgca ggaactggtt gttgggttgc
     9061 ggcgtttacc acaccttgtg ctgccgcagc catagggaac acaagttgag ttgccaggca
     9121 gatacccgca gttaagcggc gtagtttaat acccatcccg cataagattt gccggtcatt
     9181 tatttcttct ccacttctct tcttcgtagc catgcttacc ttccctgaac gagctaacat
     9241 aaatgcgccc tttctagcac agcgaaaaaa tataaatcct gatagcaata tgattgaaaa
     9301 ttaataactt ttcgttaagc agttttgggt gtgagttgca agaggggaga ctactgaatt
     9361 actcaagttt tataatcgag gggaaaatgg tgatggtgtc catagcaaaa cgccctcaac
     9421 cataaagatc gagggcgctt aagatgtcaa aaacccgcta tccgttaaaa agtaatgctc
     9481 aactaaggtc agtgacattg cgctaaaaag cgaattttta gaatttggct cctctgactg
     9541 gactcgaacc agtgacatac ggattaacag tccgccgttc taccgactga actacagagg
     9601 aatcgtgtga acgaggcgca tattacttag cggtaccttg tctgtcaaca ccaaatttca
     9661 taagttattt caactggtta attaatctgc aaacttgtta attaatgaac attcgtcgcc
     9721 gaaaacgagt ctgtatcatt agcgtgatgc agtctctgca aaggatcttg ttgataaaat
     9781 tggcagaaac gttgccacaa tgaagggaaa cgaggagcaa aaagttctgg ggcgctaaag
     9841 aaatattcag aaagtacagc aaaacactca gcaggatcac tggcagcata agcatcaatg
     9901 ctcgccgcat tctcgccaac caattcgatt tcttcctgaa tattgttcat tgcagcatga
     9961 agatcgtgtt cccagccagc aacctcacgc aacggaataa agggaactcc gctggcgcga
    10021 tcgccgttac gggtgtccag cttatgagcg acttcatgaa taatcaggtt aaaaccagaa
    10081 gcatcaaaag aatcttgtat atccaaccag ttcaaaacga taggcccttg ctgccagctc
    10141 tgacctgact gaacaatacg ttggttatgc accagaccga tatcgtcttc ccattcgtca
    10201 tcgaccacaa atggcgcagg ataaattaag acttcatgaa aaccatccag ccattccagt
    10261 cctaactcca gaacgggcag gcaaaataga agtgctatcc ggcaggttct taatgaatcc
    10321 agttcaaagc cctgtaaagg aacaagccgc ttttgctgta aaaaacgttc ggcaagagtg
    10381 actaatttgc tttgttcctg ttccgtcaga cacgttaaaa gggggatcga tagtgcttcc
    10441 tgccagggaa gggcagtttg atgtgctgat tcttgtactt tccagggcca cttaatcatc
    10501 gttttgctcg caaactcgtc acttgaacaa aattgcacgg acagggactg ttaaaatgcc
    10561 aaatttcctg gcatcatggc aaccatctga acggagagat gccggagcgg ctgaacggac
    10621 cggtctcgaa aaccggagta ggggcaactc taccgggggt tcaaatcccc ctctctccgc
    10681 cactttatca atgacttatc tcccgacttc ccgccttgct tttcctaaac agaacaatcg
    10741 tagaatattc ttgaagggtt agatcgtcac tgttttctgt tcgatactgt gacattcagc
    10801 acttgattcg ctatggatct gacaggaagc tttcgagcga aaatgtgcag ttattcagtc
    10861 gttttcttat cgctcaccat tattctttta gacattcatc ctatgaaact gccgcaaagt
    10921 tggtggtggg aactgaagtt gcgtagagaa ggggtcaata attgagtcaa aaaaggaacg
    10981 cggccgtcag attaaagccg cgggacaaag tccataattt ccaagagttg ttgaatttcc
    11041 cagctttttt gaaagccttt gtatcatact aacaagtaaa aaaggtttaa ataccaaaat
    11101 taacct
//
LOCUS       DAPGWD010000136        11101 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPGWD010000136 DAPGWD010000000
VERSION     DAPGWD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.137"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(141..1970)
                     /gene="glmS"
                     /locus_tag="RHX88_003475"
     CDS             complement(141..1970)
                     /gene="glmS"
                     /locus_tag="RHX88_003475"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDV2232128.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2132..3502)
                     /gene="glmU"
                     /locus_tag="RHX88_003476"
     CDS             complement(2132..3502)
                     /gene="glmU"
                     /locus_tag="RHX88_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDV2232129.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3853..4272)
                     /gene="atpC"
                     /locus_tag="RHX88_003477"
     CDS             complement(3853..4272)
                     /gene="atpC"
                     /locus_tag="RHX88_003477"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDV2232130.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4293..5675)
                     /gene="atpD"
                     /locus_tag="RHX88_003478"
     CDS             complement(4293..5675)
                     /gene="atpD"
                     /locus_tag="RHX88_003478"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDV2232131.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5702..6565)
                     /gene="atpG"
                     /locus_tag="RHX88_003479"
     CDS             complement(5702..6565)
                     /gene="atpG"
                     /locus_tag="RHX88_003479"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDV2232132.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6616..8157)
                     /gene="atpA"
                     /locus_tag="RHX88_003480"
     CDS             complement(6616..8157)
                     /gene="atpA"
                     /locus_tag="RHX88_003480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDV2232133.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8170..8703)
                     /gene="atpH"
                     /locus_tag="RHX88_003481"
     CDS             complement(8170..8703)
                     /gene="atpH"
                     /locus_tag="RHX88_003481"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDV2232134.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8718..9188)
                     /gene="atpF"
                     /locus_tag="RHX88_003482"
     CDS             complement(8718..9188)
                     /gene="atpF"
                     /locus_tag="RHX88_003482"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDV2232135.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9250..9489)
                     /gene="atpE"
                     /locus_tag="RHX88_003483"
     CDS             complement(9250..9489)
                     /gene="atpE"
                     /locus_tag="RHX88_003483"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDV2232136.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9536..10351)
                     /gene="atpB"
                     /locus_tag="RHX88_003484"
     CDS             complement(9536..10351)
                     /gene="atpB"
                     /locus_tag="RHX88_003484"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDV2232137.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10360..10740)
                     /gene="atpI"
                     /locus_tag="RHX88_003485"
     CDS             complement(10360..10740)
                     /gene="atpI"
                     /locus_tag="RHX88_003485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDV2232138.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2662 a         3015 c         2746 g         2678 t
ORIGIN      
        1 taaacagtaa aaaatcaagt ttgttatcag acattaatga taattcagaa tatatttata
       61 tagaaagata gatgaattta ttgatgtcag ttataaaaaa gggccaccgg tcggcagccc
      121 ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt acgcggctgg
      181 tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag cggaacggtg
      241 tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg catgttatcg
      301 ctacttacaa aacccgcatc ctgatcggcg aagacataca actgaccgcc acgcgcgcga
      361 acttcttcaa tgttggattt cagtttttcc aacagttcgt tgttcggtgc aacgacgata
      421 accggcatat ccgcgtcaat cagcgccagc gggccgtgtt tcagctcgcc tgcagcgtag
      481 gcttcagcgt gaatgtaaga gatctctttc agcttcaatg cgccttccag cgcgattggg
      541 tactgatcgc cacggcccag gaacagcgcg tgatgtttgt cagagaaatc ttctgccaga
      601 gcttcaatgc gtttgtcctg agacagcatc tgctcaatac ggctcggcag cgcctgcaga
      661 ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga cagcttcgcc
      721 accagcatca acagcacagt taactgagtg gtgaatgctt tagtggatgc cacgccgatt
      781 tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga agaacccgga
      841 acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg caggcccgcc
      901 agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt acgacgcacg
      961 gcagatttgc gatagcggaa ttcagaggcg atttcgacgt cgcacggaat acctgccagc
     1021 gattcaaacc agtaacggga aaccataccg gagttataag aagtaccaca ggcgaggatc
     1081 tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc gcttaaatca
     1141 acctgaccgt ggctgatgcg cccggtaagg gtgtttttga tcgcgttcgg ctgttcgtag
     1201 atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata ttgcagattg
     1261 gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt taccgaacgg
     1321 cgagtaattt ccgcaatatc gccctcttca aggaagataa agcgacgggt caccggcaac
     1381 agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat caccagcgga
     1441 ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat cactgtaccg
     1501 tacgcaccac gcagttgcgg gatagcacgc agaacagcct cacgcagagt accgccctgt
     1561 ttcagctccc agttcaccag atgggcaatt acttcggtgt ctgtttcaga aacgaaggta
     1621 tagccacgcg cttttaactc ttcgcgcagc ggttcatggt tttcgatgat gccgttatgc
     1681 accaccacaa tgtgttcaga aacatgcgga tgcgcattca cttctgaagg ttcaccgtgg
     1741 gtcgcccagc gagtatgagc aatacccgta ccgccatgca gaggatgttc ttccgctgcc
     1801 tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc ttccgcatca
     1861 acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa accttcaaga
     1921 aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat agtttttgat
     1981 tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc cccgagcctt
     2041 gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat gttatctcct
     2101 catcccatgt gaccggatta cccggccaga atcacttttt ctttaccgga cgacgccagc
     2161 cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg acattacgcg
     2221 tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg gccaccagct
     2281 gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta ttcgcaccat
     2341 cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt tccgcatcgc
     2401 ccaggtaagt cagatgacca gctttcgagc ctttacccag acgcgctttt ttcatctcaa
     2461 caaagttacc gacgtgtgca ccttccagca actcagcacc aggacgcagt cgggcaaacg
     2521 ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac ggactgattt
     2581 cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt ttcacgcgat
     2641 gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca tcgcgcccgt
     2701 gagtaagcgt accgcgcaaa tcaaaacgcg ccggatcgcg cagcataacg cctgctaaca
     2761 gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt tgcaggcggt
     2821 tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt tcacgccctt
     2881 cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca ttattgttgg
     2941 tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg ccggtgttga
     3001 tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg ccggtaactt
     3061 tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc accgtcagca
     3121 gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg acagagatca
     3181 gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa ggtgcggcct
     3241 gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta aggttgtcgt
     3301 ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaggtga acgtgcgctg
     3361 cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg gcaagggtat
     3421 gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca aggatcacta
     3481 cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga atttaaaccg
     3541 cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat aaattgttca
     3601 ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg ccttttgtct
     3661 gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg ctcttatttc
     3721 gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta tgtgaacgct
     3781 cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc ttttgtgctt
     3841 ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct gcgcgatcgc
     3901 tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc tgctaatgtg
     3961 ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct ggccgcgaat
     4021 tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc cgccagacag
     4081 atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag gcttaatggc
     4141 ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac ccgttacctg
     4201 gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt ccaggtggta
     4261 agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt ttccacagct
     4321 tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta ttcgccttcc
     4381 atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc cggagaaccg
     4441 gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt acgagcacgc
     4501 gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc gatgatgtct
     4561 ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc gtagtgttcc
     4621 tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc aacggccggg
     4681 tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag gtgcgcaaag
     4741 gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc ctgtacggag
     4801 gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat ctcttccgcc
     4861 agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga tacttccgta
     4921 ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg accttcgtca
     4981 cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt tcccggcggc
     5041 tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc ggtcatttcg
     5101 tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac agagtaaccg
     5161 gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt acctacaccc
     5221 gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag gtcgataact
     5281 ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga aggtgctgcg
     5341 cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac cggttcaccc
     5401 agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat cgggtgttcg
     5461 aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc gatggtacgc
     5521 acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt accattttgc
     5581 acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac gtcaactacg
     5641 gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg aaataacctg
     5701 tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg ctggcctgac
     5761 gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg tcggtcgcgg
     5821 ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc acgccctgat
     5881 aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc ggatcgggtt
     5941 cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc ggtaacggca
     6001 gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg ctgacaatgt
     6061 aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc ggaccgatca
     6121 gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca ttgccgccca
     6181 cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat tgaacgcctt
     6241 tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg ttcaaaccac
     6301 cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg tcgcggtctt
     6361 ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc actttgcgca
     6421 tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc attttggaag
     6481 cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt gcgatcttac
     6541 tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc ctgccctaag
     6601 gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga tgcctttcag
     6661 cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca tcaacggagc
     6721 gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt tcgacagttc
     6781 aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct gcgcaacgga
     6841 catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt ggtcaagctg
     6901 cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca gttcacgata
     6961 ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg tctgtgctgc
     7021 accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac cggcgttgaa
     7081 caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg tcggaacgaa
     7141 cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag aaccggtttt
     7201 ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac gtgcagcacg
     7261 ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac gtcctggcgg
     7321 acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca ggtcatcgta
     7381 aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc aaccggcata
     7441 cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa cgatggtgtt
     7501 agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg acgctttctg
     7561 gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga tggcatcgat
     7621 agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct gaccacgacc
     7681 gattgggatc atggagtcaa cggctttata accggtctgt accggctgat ctacggactg
     7741 acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt gatccagcgg
     7801 acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca ggccacggcc
     7861 aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg caaggtcagc
     7921 gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg cgatagcgta
     7981 acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc cgtgaatgcg
     8041 gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac tcacaacatt
     8101 gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca gttgcatgct
     8161 ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc gtacgctgcc
     8221 atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact tatcgatttt
     8281 gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa ttttcgcgag
     8341 ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag cctcactcac
     8401 ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac gaccattttc
     8461 agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac aaactgcgat
     8521 aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg ccatttgttc
     8581 gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta cactttggtg
     8641 ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa taaattcaga
     8701 catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc gctgttagca
     8761 gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag gatagcaact
     8821 tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc ctgcgcctgg
     8881 gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag aatctgcgag
     8941 cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt tttcagctgg
     9001 tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc ggaagcaagg
     9061 ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg ccatacgtac
     9121 ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat tgttgcgtta
     9181 agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt taaataaaag
     9241 caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag cgatcatcgg
     9301 gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag gaatcagatc
     9361 aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac cgatcgcagc
     9421 accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat ccatattcag
     9481 gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg gtaaattaat
     9541 gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg aaggcttgca
     9601 gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac tgtgaccacc
     9661 acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg ttaccgaaca
     9721 gtcgcaaacc aagtgaaact ggtttggaca gcaggcttac cccttcaagg attaagttga
     9781 caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg aagccgccga
     9841 tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt gccatagaca
     9901 gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc agtacatgtt
     9961 cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc aggaataccc
    10021 agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg tacatgtctt
    10081 tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac ttacctggca
    10141 caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac agacccagca
    10201 ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg gggttttgtg
    10261 gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg tgtcctatgt
    10321 aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt tgttaattac
    10381 agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca gcggcaagaa
    10441 taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca gaactttgaa
    10501 agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat gcgcctggtg
    10561 acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc ccccgcttat
    10621 tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg ctatcaccac
    10681 cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg acacagacat
    10741 cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa ctttctttaa
    10801 ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg tgatttcaaa
    10861 caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat acaattgcga
    10921 cttttctgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa cactggtgat
    10981 aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta tttacttggc
    11041 aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg ttttaataaa
    11101 t
//
LOCUS       DAPGWD010000137        10936 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPGWD010000137 DAPGWD010000000
VERSION     DAPGWD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.138"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            184..1398
                     /locus_tag="RHX88_003486"
     CDS             184..1398
                     /locus_tag="RHX88_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HDV2232139.1"
                     /translation="MKIIAADVFVTCPGRNFVTLQITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASCIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1465..2748
                     /locus_tag="RHX88_003487"
     CDS             1465..2748
                     /locus_tag="RHX88_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2232140.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3000..3917
                     /gene="oxyR"
                     /locus_tag="RHX88_003488"
     CDS             3000..3917
                     /gene="oxyR"
                     /locus_tag="RHX88_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDV2232141.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3900..5300)
                     /gene="sthA"
                     /locus_tag="RHX88_003489"
     CDS             complement(3900..5300)
                     /gene="sthA"
                     /locus_tag="RHX88_003489"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDV2232142.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5631..6281
                     /gene="fabR"
                     /locus_tag="RHX88_003490"
     CDS             5631..6281
                     /gene="fabR"
                     /locus_tag="RHX88_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDV2232143.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6281..6640
                     /locus_tag="RHX88_003491"
     CDS             6281..6640
                     /locus_tag="RHX88_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDV2232144.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6680..7780)
                     /gene="trmA"
                     /locus_tag="RHX88_003492"
     CDS             complement(6680..7780)
                     /gene="trmA"
                     /locus_tag="RHX88_003492"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDV2232145.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7894..8084
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8149..9993
                     /gene="btuB"
                     /locus_tag="RHX88_003493"
     CDS             8149..9993
                     /gene="btuB"
                     /locus_tag="RHX88_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDV2232146.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9938..10795
                     /gene="murI"
                     /locus_tag="RHX88_003494"
     CDS             9938..10795
                     /gene="murI"
                     /locus_tag="RHX88_003494"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDV2232147.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2508 a         2593 c         2925 g         2910 t
ORIGIN      
        1 taacgtgttg ttttaacgat ggtagttaat tattcaggtg gtttttgagg gtattttttc
       61 tcctcggcgg aaatttttat ttttaatgtg atcacggttg aataatcgct ctttctctct
      121 tgtatggtag taggcgtaaa attaagtttg aagcgtctat ttacaacaca ggagattgca
      181 ataatgaaaa ttattgctgc agatgttttt gttacttgcc cagggcgtaa tttcgttacg
      241 cttcaaataa ccactgaaag cggactttgt ggattaggag atgccacgtt aaatggtcgc
      301 gaattgtcgg tcgcttctta tctgaaagat catctttgtc cacaactaat tggccgcgat
      361 gccagctgta tcgaagatat ctggcaattc ttttataaag gtgcttactg gcgacgtggc
      421 cccgtcacga tgtcagccat ttctgccatt gatatggcgt tgtgggatat caaagctaaa
      481 gcggcaaata tgccattgta tcaactgctt ggtggtgctt ctcgtgaagg cgtgatggtt
      541 tattgccaca ccaccgggcg gacgattgat gaagtccttg aagactatgc aaagcaccag
      601 cagatgggct ttaaggcgat tcgtgtgcag tgcggtgtac cgggtatgca aactacttat
      661 ggcctggcga agggcaaagg cctggcgtat gaaccggcaa ccaaaggcct gtggccagaa
      721 gagcagttgt ggtcttcaga aaagtatctc gattttacgc cgaagttatt tgaagcggtg
      781 cgtaataagt tcggttttaa cgaacatctg ttgcatgaca tgcaccatcg cctgaccccc
      841 atcgaagcgg cgcgctttgg gaagagcatt gaggattacc gcctgttctg gatggaagat
      901 ccgacgcctg cagagaacca ggaatgtttt cgtctgattc gccagcatac cgtgacgccg
      961 attgcggtcg gtgaagtgtt taacagtatc tgggactgca agcagttaat cgaagagcag
     1021 ctgattgact atattcgcac gacgatcacc catgcaggcg gcattaccgg gatgcggcgg
     1081 attgccgatt ttgcatcgct ttatcaggta cgtaccggtt cgcatggccc ttcagattta
     1141 tcgcctgttt gtcatgcggc ggcgctgcat tttgacctat gggtaccaaa ttttggcgtt
     1201 caggagtaca tgggctattc cgaacaaatg cttgaagttt tcccgcacag ctggcgcttc
     1261 gaggaaggtt atatgcaccc aggcgatgaa ccggggctgg gtatttcttt cgatgagaaa
     1321 ctggcggcga aatatccgta tgacccggcg tatctccccg tcgcacgact ggaagatggc
     1381 acattgtgga actggtaata acggttcgct taattatttt ctgaatagtc atcaaatcga
     1441 ttaattccgc tcaaggagct aaaaatggac gttgatgtgt caaccagtgt tgcgggtaat
     1501 aaaccgcaac ggattcgtcg tattcagaca gtgaccctgg ttttattatt tatggcgggg
     1561 atcgttaatt ttctcgaccg ttcgtcattg agcgtggcag gtgaagcgat tcgtggcgag
     1621 ttaggattat cggcgacaga gtttggtgtt ttgctttctg cattttctct gtcttatggt
     1681 ttttcacaac taccttccgg tattttgcta gaccgctttg gcccacgaat tgttttaggc
     1741 gcaggcttaa tattctggtc attaatgcag gcattaacag gaatggttaa ttcttttagc
     1801 cactttattt taatgcgtat cggtctgggt attggcgaag cgccatttat gcctgcggga
     1861 gtaaagtcga tcaccgactg gtatgcacaa aaagaacgcg gcacggcgct ggggattttt
     1921 aactcatcta ccgttatcgg tcaggctatc gcgcctcctg ctcttgtatt gatgcagctg
     1981 gcatggggct ggcggacgat gttcgttatc atcggcgtgg cagggattct ggttgggatc
     2041 tgttggtacg cgtggtatcg caaccgggcg cagtttgtcc tgactgacga agaacggacg
     2101 tatctctccg cgccggttaa accgcgtcca cagctgcaat ttagcgagtg gctggcgctg
     2161 tttaagcatc ggacaacctg ggggatgatt ttaggtttct ctggcgtcaa ttataccggt
     2221 tggctctaca tcgcgtggtt acccggttat ttgcaagccg agcaaggttt cagtctggcg
     2281 aaaaccggtt gggtggcggc gattcctttc ctcgcggcgg cagtcgggat gtgggttaac
     2341 ggtattgttg tcgatcgact ggcgaaaaaa ggctacgacc tggcgaagac gcgtaaaacg
     2401 gctattgtct gcggtttgat gatgtcggca ttaggcacgt tgctggtcgt gcaatcttcc
     2461 tcgccagccc aggcggtagc gtttatctca atggcgctat tctgcgtgca tttcgctgga
     2521 acgtctgcat gggggctggt gcaggtgatg gtgtcagaaa caaaagtggc ttccatcgcc
     2581 gggattcaaa actttggcag ttttgttttt gcttcctttg ctccgatcgt aaccggttgg
     2641 gtagtggata ccacacactc gtttaatctg gcgttggtta tcgcggcctg tgtgacgttc
     2701 accggggcgc tgtgttactt ctttattgtc aaagatcgca ttgagtaatc gttactatca
     2761 ggctctcttg ctgtgggcct gtagaataaa aaaaagcgga tcctggagat ccgcaaaagt
     2821 tcacgttggc tttagttatt cgagttgaga aactctcgaa acgggcagtg acttcaaggg
     2881 ttaaaagagg tgccgctccg tttctgtgag caattatcag tcagaatgct tgatagggat
     2941 aatcgttcat tgctattcta cctatcgcca tgaactatcg tggcaatgga ggatggataa
     3001 tgaatattcg tgatcttgag tacctggtgg cattggccga acaccgccat tttcggcgtg
     3061 cggcagattc ctgccacgtt agccagccga cgcttagcgg gcaaattcgt aagctggaag
     3121 atgagttggg cgtgatgttg ctggagcgga ccagccgtaa agtgttgttc acccaggcgg
     3181 gaatgctgct ggtagatcag gcgcgtaccg tactgcgtga ggtgaaagtc cttaaagaga
     3241 tggcaagcca gcagggcgag acgatgtccg ggccgctgca cattggtttg attcccacag
     3301 ttggaccgta cctgctaccg catattatcc ctatgctgca ccagaccttt ccaaagctgg
     3361 aaatgtatct gcatgaagca cagacccacc agttactggc gcaactggac agcggcaaac
     3421 tcgattgcgt gatcctcgcg ctggtgaaag agagcgaagc attcattgaa gtgccgttgt
     3481 ttgatgagcc aatgttgctg gctatctatg aagatcaccc gtgggcgaac cgcgaatgcg
     3541 taccgatggc cgatctggca ggggaaaaac tgctgatgct ggaagatggt cactgtttgc
     3601 gcgatcaggc aatgggtttc tgttttgaag ccggggcgga tgaagataca cacttccgcg
     3661 cgaccagcct ggagacgctg cgcaacatgg tggcggcagg tagcgggatc actttactgc
     3721 ctgcgctggc tgtgccgccg gagcgcaaac gcgatggggt tgtttatctg ccgtgcatta
     3781 agccggaacc acgccgcact attggcctgg tttatcgtcc tggctcaccg ctgcgcagcc
     3841 gctatgagca gctggcagag gccatccgcg cacgaatgga tggccatttc gataaagtgt
     3901 taaaacaggc ggtttaaacc gtttaacgca gctacccgat aggcttccgc catggtcggg
     3961 tagttaaagg tggtgttgac gaagtactca atagtgttgc cgccaccttt ctgttccata
     4021 atcgcctgac cgatatgaat aatttcggca gcgcgctcgc caaagcagtg aatacccaga
     4081 atctcttttg tttcccgatg gaacaaaatt ttcagcgtgc ccacgttcat gccgacgatt
     4141 tgtgcgcgtg ccagatgttt aaactgggcg cggcccactt catatggcac tttcatcgcg
     4201 gtcagctgct gttcggtttt gcccacagag ctgatttccg ggatggtgta aatgccggta
     4261 gggatatctt caatcagatg cgcggtggct tcgcctttta ccagcgcctg cgcggcaatg
     4321 cgcccctggt cataggccgc tgatgccagg ctcggataac caatcacgtc gcccaccgcg
     4381 taaacgtgcg gctgtgcggt ctgatacatg ctgttgacct tcagctgtcc gcgactgtca
     4441 gtttccagcc caatgttctg taacgccagc gaatcggtat taccggtacg accgttggca
     4501 tagagcaggc agtcagcttt cagtttttta cccgacttca gatgcatgat cacaccgtcg
     4561 tcacagcctt cgatcttctc gtactcttcg ttgtgacgaa tcactacgcc actgttccag
     4621 aagtgatagg agagagaatc tgacatctct tgatcgagaa atgccagcag gcgatcgcgg
     4681 gtgttgatca gatccacttt tacatccata ccgcggaaga tcgacgcata ttcacagccg
     4741 atcactccag caccatagat aagtacatgg cgcggttcgt ggtgcatgct aagaattgag
     4801 tcgctgtcgt aaatgcgtgg atgggtgaaa tcaacatctg ttggatgata tggacgagag
     4861 ccgcaggcaa taacaaattt ttcagcggtt agtgtttcaa cgctgccgtc cgggcaatcc
     4921 agcgccaacg tatgctcgtc aacaaagcga gcgtttccct gcaatatttc acagtgatta
     4981 cgttcgtaaa atccctgacg catgcgcgtt tgttgattaa tcacgttatc ggcatggtta
     5041 aggatatcgg caaaagaaga gcggagcagt cgggaatggt cgctgtaaag tgggttttga
     5101 ttgaattcta taatgcggct gacggcgtga cggagagctt tcgacgggat ggtgccccag
     5161 tgggtgcaac cgccgccaac attttgataa cgctcgataa ctgcgacgcg cgcaccttgc
     5221 ttaaccaggc ccattgcagc gccttcgccg ccggggccgg aacctattac tatggcatcg
     5281 taatcgtagg aatgtggcat ggtagggctt acctgttctt atacataaaa gcaacagaat
     5341 ggtaacattt tatcgcgggt aagccaattg atccccgtca tttatctggc tatatcctga
     5401 gcggcctttg ctttgtctgt ttcttacttt tgccctgacg ttttattgga tttttatcga
     5461 cgatactctc cgtttaagcg gcaggtttcc gctgtacgta aaagaaccgg ccaaagaatt
     5521 gcagtaaata tgttttattg cgttaccgtt cattcacaat actggagcaa tccagtatgt
     5581 tcattctctg gtatagtgcc agcagtactt ttggcaagga ttcagacatc gtgatgggcg
     5641 taagagcgca acaaaaagaa aaaacccgcc gttcgctggt ggaagccgca tttagccaat
     5701 taagtgctga acgcagcttc gccagcctga gtttgcgtga agtggcgcgt gaagcgggca
     5761 ttgctcccac ctctttttat cggcatttcc gcgacgtaga cgaactgggt ctgaccatgg
     5821 ttgatgagag cggtttaatg ctacgccaac tcatgcgcca ggcgcgtcag cgtatcgcca
     5881 aaggcgggag tgtgatccgc acctcggtct ccacatttat ggagttcatc ggtaataatc
     5941 ctaacgcctt ccggttatta ttgcgggaac gctccggcac ctccgctgcg tttcgtgccg
     6001 ccgttgcgcg tgaaattcag cacttcattg cggaacttgc ggactatctg gaactcgaaa
     6061 accatatgcc gcgtgcgttt actgaagcgc aagccgaagc aatggtgaca attgtcttca
     6121 gtgcgggtgc cgaggcgttg gacgtcggcg tcgaacaacg tcggcaatta gaagagcgac
     6181 tggtactgca actgcgaatg atttcgaaag gggcttatta ctggtatcgc cgtgaacaag
     6241 agaaaaccgc aattattccg ggaaatgtga aggacgagta atgaaacaag caaatcaaga
     6301 tagaggtacg ctgctgctgg cgttggttgc tggcttatcg attaatggta ctttcgcagc
     6361 gctgtttagc tccattgtgc cattttctgt attcccgatt atttccctgg tgctgacggt
     6421 ttactgcctg catcaacgtt atcttaatcg caccatgccg gtaggcttgc cgggtctggc
     6481 agctgcctgt tttattctcg gcgtactgct gtacagcacg gtagttcgtg cggaatatcc
     6541 ggatatcggc tctaacttct tcccggcagt actctccgtc attatggtgt tctggattgg
     6601 cgcgaagatg cgtaaccgta agcaggaagt tgctgagtaa tcggtattat gccggatgct
     6661 ggcgcatccg gcatgggttt tacttcgcgg tcagtaatac gccgcactcc atatggtgcg
     6721 tgtaggggaa ctgatcaaac agagccagac gttcgacctt gtgcgtctgg cttaatgttt
     6781 ccagattctt gcataacgtt tccggattgc aggagatata cagaatacgt ggatacgcct
     6841 gcaccatttt ctcggtttca ctgtccagac cgctgcgcgg tgggtcgaca aaaatggttt
     6901 cgcactgata actcttcaag tctatccctt gcaggcgatt aaactcgcgt acaccattca
     6961 tcgcctgagt aaattcttct gccgccatac gaataatttg tacgttatca atatggttag
     7021 ctgcgatgtt gtattgcgca gcagcaaccg acggcttagc gatttcggtg gctaataccc
     7081 gatcaaaatt gcgcgccagt gctaatgaaa agttaccgtt gccgcagtac agctccagta
     7141 aatcgccttt tgagcctttg gttacgtcca gcgcccattc cagcatctga atattcatcg
     7201 ccgcgttcgg ctgggtaaaa ctgttttcta cctgacggta gatcatctct ttccctgcga
     7261 ccggcagacg ttcatcgatg taatcttgat ccagctcgat tttggttttc gttgcccgac
     7321 caatcagatg cacattcaga ttctgcgcgc gcagtgcatc gcgcagggct tccgcttcct
     7381 gacgccactc atcatccagc tttttatggt acaacaggga aaccaccgcc tgattactca
     7441 gcgtggtgag gtaatcaatc tggaacaact tgtggcgcag aacgggatta ttacgcacac
     7501 ccgcaatcat cgccgtcatc aactggttga tgagttcact ggcagcgggg aagctatcca
     7561 cgcggatgcg gcttttggtt tgttgatcga aaatgatgtg atacaggtca tcgccatcgt
     7621 gccagatgcg gaactccgca cgcatccggt aatgactgac cggcgagcga aacacttccg
     7681 gaaccaggtc agaaaacggt gccatcatac tttgcaaacg taccactttt tcggctaact
     7741 gcgcttcata ctgttctgtt ggaaggtgtt cgggggtcat gatgtatcct gaaaaattaa
     7801 agtacgcggc gattgtaggg attgctcatc agatgtccag atcttgatga attcctattt
     7861 gtgagctacg tctggacagt aacttgttac aacctgtagc atccacttgc cggtcctgtg
     7921 agttaatagg gaatccagtg tgaatctgga gctgacgcgc agcggtaagg aaaggtgcga
     7981 tgattgcgtt atgcggacac tgccatccgg tgggaagtca tcatctctta gtatcttaga
     8041 tacccctcca agcccgaaga cctgccggcc aacgtcgcat ctggttctca tcatcgcgta
     8101 atattgatga aacctgcggc atccttcttc tattgtggat gctttacaat gattaaaaaa
     8161 gcttcgctgc tgacggcgtg ttctgtcaca gccttttccg cttgggcaca ggataccagc
     8221 ccggatactc tcgtcgttac tgctaaccgt tttgaacagc cgcgcagcac tgtgcttgca
     8281 ccaaccaccg ttgtgacccg tcaggatatc gaccgctggc agtcgacctc ggtcaatgat
     8341 gtgctgcgcc gtcttccggg cgtcgatatc acccaaaacg gcggttcagg tcagctctca
     8401 tctattttta ttcgcggtac aaatgccagt catgtgttgg tgttaattga tggcgtacgc
     8461 ctgaatctgg cgggggtgag tggttctgcc gaccttagcc agttccctat tgcgcttgtc
     8521 cagcgtgttg aatatatccg tgggccgcgc tccgctgttt atggttccga tgcaataggc
     8581 ggggtggtga atatcatcac gacgcgcgat gaacccggaa cggaaatttc agcagggtgg
     8641 ggaagcaata gttatcagaa ctatgatgtc tctacgcagc aacaactggg ggataagaca
     8701 cgggtaacgc tgttgggcga ttatgcccat actcatggtt atgatgttgt tgcctatggt
     8761 aataccggaa cgcaagcgca gacagataac gatggttttt taagtaaaac gctttatggc
     8821 gcgctggagc ataactttac tgatgcctgg agcggctttg tgcgcggcta tggctatgat
     8881 aaccgtacca attatgacgc gtattattct cccggttcac cgttgctcga tacccgtaaa
     8941 ctctatagcc aaagttggga cgccgggctg cgctataacg gcgaactgat taaatcacaa
     9001 ctcattacca gctatagcca tagcaaagat tacaactacg atccccattt tggtcgttat
     9061 gattcgtcgg cgacgctcga tgagatgaag caatacaccg tccagtgggc aaacaatgtc
     9121 atcgttggtc acggtagtat tggtgcgggt gtcgactggc agaaacagac tacgacgccg
     9181 ggtacaggtt atgttgagaa tggatatgat caacgtaata ccggcatcta tctgaccggg
     9241 ctgcaacaag tcggcgattt tacctttgaa ggcgcagcac gcagtgacga taactcacag
     9301 tttggtcgtc atggaacctg gcaaaccagc gccggttggg aattcatcga aggttatcgc
     9361 ttcattgctt cctacgggac atcttataag gcaccaaatc tggggcaact gtatggcttc
     9421 tacggaaatc cgaatctgga cccggagaaa agcaaacagt gggaaggcgc gtttgaaggc
     9481 ttaaccgctg gggtgaactg gcgtatttcc ggatatcgta acgatgtcag tgacttgatc
     9541 gattatgatg atcacaccct gaaatattac aacgaaggga aagcgcggat taagggcgtc
     9601 gaggcgaccg ccaattttga taccggacca ctgacgcata ctgtgagtta tgattatgtc
     9661 gatgcgcgca atgcgattac cgacacgccg ttgttacgcc gtgctaaaca gcaggtgaaa
     9721 taccagctcg actggcagtt gtatgacttc gactggggta ttacttatca gtatttaggc
     9781 actcgctatg ataaggatta ctcatcttat ccttatcaaa ccgttaaaat gggcggtgtg
     9841 agcttgtggg atcttgcggt tgcgtatccg gtcacctctc acctgacagt tcgtggtaaa
     9901 atagccaacc tgttcgacaa agattatgag acagtctatg gctaccaaac tgcaggacgg
     9961 gaatacacct tgtctggcag ctacaccttc tgaaccacgt cccaccgtgc tggtgtttga
    10021 ctccggcgtc ggtgggttgt cggtctatga cgagatccgg catctcttac cggatctcca
    10081 ttacatttat gctttcgata acgtcgcttt tccgtatggc gaaaaaagcg aagagtttat
    10141 tgttgagcga gtggtggcaa ttgtcaccgc agtgcaagaa cgttatcccc ttgcgctggc
    10201 tgtggtcgct tgcaacaccg ccagtaccgt ttcacttcct gcattacgcg aaaagttcga
    10261 cttcccggtt gttggtgtcg tgccggcgat taaacctgct gcacgtctga cggcaaatgg
    10321 cattgtcgga ttactggcaa cccgcggaac tgttaaacgt tcttatactc atgagctgat
    10381 cgcgcgtttc gctaatgaat gccagataga aatgctgggc tcggcagaga tggttgagtt
    10441 ggctgaagcg aagctgcatg gcgaagatgt ttctctggat gcactaaaac gtatcctacg
    10501 cccgtggtta agaatgaaag agccgccaga taccgttgta ttgggttgca cccatttccc
    10561 tctactacaa gaagaactct tacaggtgct gccagaggga acccggctgg tggattctgg
    10621 cgcagcgatt gctcgcagaa cggcctggct gttagaacat gaagctccgg atgcaaaatc
    10681 tgccgatgcg aatattgcct tttgtatggc aatgacgcca gaagctgaac aattattgcc
    10741 cgttttacag cgttacggct tcgaaacgct cgaaaaactg gcagttttag gctgatttgg
    10801 ttgaatgttg cgcggtcaga aaattatttt aaatttcctc ttgtcaggcc ggaataactc
    10861 cctataatgc gccaccactg acacggaaca acggcaaaca cgccgccggg tcagcggggt
    10921 tctcctgaga actccg
//
LOCUS       DAPGWD010000138        10917 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPGWD010000138 DAPGWD010000000
VERSION     DAPGWD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.139"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            100..714
                     /gene="evgA"
                     /locus_tag="RHX88_003495"
     CDS             100..714
                     /gene="evgA"
                     /locus_tag="RHX88_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDV2232148.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            719..4312
                     /gene="evgS"
                     /locus_tag="RHX88_003496"
     CDS             719..4312
                     /gene="evgS"
                     /locus_tag="RHX88_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDV2232149.1"
                     /translation="MKFLPYFFLLCCGLWSTISYADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4368..5513)
                     /gene="yfdE"
                     /locus_tag="RHX88_003497"
     CDS             complement(4368..5513)
                     /gene="yfdE"
                     /locus_tag="RHX88_003497"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDV2232150.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5587..6531)
                     /gene="yfdV"
                     /locus_tag="RHX88_003498"
     CDS             complement(5587..6531)
                     /gene="yfdV"
                     /locus_tag="RHX88_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDV2232151.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6601..8295)
                     /gene="oxc"
                     /locus_tag="RHX88_003499"
     CDS             complement(6601..8295)
                     /gene="oxc"
                     /locus_tag="RHX88_003499"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDV2232152.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8349..9599)
                     /gene="frc"
                     /locus_tag="RHX88_003500"
     CDS             complement(8349..9599)
                     /gene="frc"
                     /locus_tag="RHX88_003500"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDV2232153.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10112..10744)
                     /locus_tag="RHX88_003501"
     CDS             complement(10112..10744)
                     /locus_tag="RHX88_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDV2232154.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3199 a         2457 c         2254 g         3007 t
ORIGIN      
        1 gctcagattt tcttattttt attgagaaaa tgagatgacg ccttatgtct gtattactac
       61 agggagaagg gagatgcttc attgcaaagg gaataatcta tgaacgcaat aattattgat
      121 gaccatcctc ttgctatcgc agcaattcgt aatttattga tcaaaaacga tattgaaatc
      181 ttagcggagt tgactgaagg gggaagcgcc gttcagcggg tggaaacact taagcctgat
      241 atcgtcatca ttgatgtcga tatccccgga gttaacggta tccaggtgtt agaaacgctg
      301 aggaagcgcc aatatagcgg aattattatt attgtctccg ctaaaaatga ccatttttac
      361 gggaaacatt gtgctgatgc tggcgctaat ggattcgtga gtaaaaaaga aggcatgaac
      421 aatatcattg cggctattga agctgcaaaa aatggctact gctatttccc cttctctctc
      481 aaccggtttg ttggaagttt aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa
      541 gaaattagtg tcatgcggta tattcttgat ggcaaggata ataatgacat tgctgaaaaa
      601 atgttcatca gcaacaaaac tgtcagcact tataaaagtc gcctgatgga aaaattagaa
      661 tgtaaatcac tgatggatct ttacacattc gcgcaacgta acaaaatcgg ctaatcacat
      721 gaagttttta ccctattttt ttcttctctg ttgtggtctt tggtcgacca taagttacgc
      781 agacgaagat tacatcgaat atcgtggcat cagtagtaac aaccgtgtca cacttgatcc
      841 agtacgtctg agcaacaagg aattacgttg gttagcgagc aaaaaaaatc ttgtgattgc
      901 agtacacaag tcccaaacgg ctaccttgtt gcataccgat tcgcagcaac aggttcgtgg
      961 tattaatgct gattatttaa atcttttaaa aagagcgtta aatatcaaat taacactccg
     1021 ggaatacgca gatcatcaaa aagcaatgga cgcgctggaa gaaggtgaag tcgatatagt
     1081 gttatcacat ttagttactt cgccgcctct taataatgac attgctgcaa ccaaaccatt
     1141 gataattacc tttccggcgc tggtaaccac ccttcacgac tcaatgcgac cgcttacctc
     1201 accaaaacca gtaaatattg ctcgggtagc aaattacccc ccagacgagg taattcatca
     1261 atcgtttcca aaagcaacaa ttatctcttt tacaaattta tatcaggcat tagcatccgt
     1321 atcagccgga cagaatgatt actttattgg tagtaacatc attaccagca gtatgatttc
     1381 ccgctatttc actcactcct taaatgtagt gaaatattat aactcgccgc gtcaatataa
     1441 ttttttcttg accagaaaag aatccgtcat tcttaatgaa gtactcaata gatttgttga
     1501 tgctttaaca aatgaagttc gctatgaagt ttcacaaaat tggcttgata caggaaacct
     1561 ggcctttctg aacaaaccat tagaactcac tgaacatgaa aaacagtgga ttaagcaaca
     1621 tcccgattta aaggtgctgg aaaatcctta ctctcctccc tattctatga cggatgaaaa
     1681 tggctcggtt cggggcgtta tgggggatat tcttaatatt attaccttgc aaacaggttt
     1741 aaatttttct ccgatcaccg tttcacacaa tatccatgcc ggaacacagc ttaaccccgg
     1801 tggatgggat ataatacctg gcgctattta tagtgaagat cgagaaaata atgtttcatt
     1861 tgcagaagct ttcataacaa cgccatacgt ttttgtcatg caaaaagcac ctgacagtga
     1921 acaaacatta aaaaaaggaa tgaaagttgc cattccatat tattatgagc tgcattcgca
     1981 attaaaagag atgtatccgg aggttgaatg gatacaggtc gataatgcca gcgctgcatt
     2041 tcacaaggtt aaggaaggtg aacttgatgc tctggtcgcg acacagttaa attcgcgtta
     2101 catgatcgat cattactatc ctaatgaact ttatcatttt cttattcctg gcgttccgaa
     2161 tgcatcgctt tcgttcgctt ttcctcgcgg agaaccggaa cttaaggata ttattaataa
     2221 agcactgaat gcaattcccc caagcgaagt tctgcgcctg acggaaaaat ggattaaaat
     2281 gcccaatgtg accattgaca catgggacct atatagcgag caattttata ttgttacgac
     2341 attatccgtt ttattagttg gcagtagcct tttatgggga ttctacctgt tacgctcagt
     2401 tcgtcgtcgt aaagtcattc agggtgattt agaaaaccaa atatcattcc gaaaagcact
     2461 ctcggattcc ttaccgaatc caacttatgt tgtaaactgg caaggtaatg tcattagtca
     2521 taatagtgct tttgaacatt atttcactgc ggattactac aaaaatgcaa tgttaccatt
     2581 agaaaacagt gactcaccct ttaaagatgt tttttctaat gcgcatgaag tcacagcaga
     2641 aacgaaagaa aatcgaacaa tatacacaca ggtatttgaa attgataatg gcatcgagaa
     2701 aagatgcatt aatcactggc atacattatg caatcttcct gcaagtgaca atgcagtata
     2761 tatttgtggt tggcaagata ttactgaaac gcgtgatcta attaatgcac tcgaggtaga
     2821 aaaaaataaa gcgataaagg ctaccgtagc aaaaagtcag tttctggcaa cgatgagtca
     2881 cgaaataaga acaccaataa gctctattat gggcttcctg gaacttctgt cgggttctgg
     2941 tcttagcaag gagcaacggg tggaggcgat ttcacttgcc tacgccaccg gacaatcact
     3001 cctcggctta attggtgaaa tccttgatgt cgacaaaatt gaatcgggta actatcaact
     3061 tcaaccacaa tgggtcgata tccctacttt agtccagaac acttgtcact ctttcggtgc
     3121 gattgctgca agcaaatcga tcgcattaag ttgcagcagt acatttcctg atcattacct
     3181 ggttaagatc gaccctcagg cgtttaagca ggtcttatca aatttactga gcaatgctct
     3241 caaatttacc accgaggggg cagtaaaaat tacgacctcc ctggttcaca ttgatgacaa
     3301 ccacgctgtt atcaaaatga cgattatgga ttctggaagt ggattatcgc aggaagaaca
     3361 acaacaactc tttaaacgct acagccaaac aagtgcaggt cgccagcaaa caggatctgg
     3421 cttaggctta atgatctgca aagaattaat taaaaatatg cagggcgatt tgtcattaga
     3481 aagtcatcca ggcataggaa caacatttac gatcacaatc ccggtagaaa ttagccagca
     3541 agtggcgact gtcgaggcaa aagcagaaca ccccatcaca ctacctgaaa agttgagcat
     3601 attaatcgcg gatgatcatc cgaccaacag gctattactc aaacgccagc taaatctatt
     3661 aggatatgat gttgatgaag ccactgatgg tgtgcaagcg ctacacaaag tcagtatgca
     3721 acattatgat ctgcttatta ctgacgttaa tatgccgaat atggatggtt ttgagttgac
     3781 tcgcaaactc cgtgagcaaa attcttcctt acccatctgg gggcttacag ccaacgcaca
     3841 ggctaacgaa cgtgaaaaag ggttaagttg cggcatgaac ttatgtttgt tcaaaccgtt
     3901 gaccctggat gtactgaaaa cacatttaag tcagttacac caggttgcgc atattgcacc
     3961 tcagtatcgc caccttgata tcgaagccct gaaaaataat acggcgaacg atctacaact
     4021 gatgcaggag attctcatga ctttccagca tgaaacgcat aaagatctac ccgctgcgtt
     4081 tcaagcacta gaagctggcg ataacagaac tttccatcag tgtattcatc gcatccacgg
     4141 tgcggctaac atcctgaatt tgcaaaagtt gattaatatt agccatcagt tagaaataac
     4201 acctgtttca gatgacagta agcctgaaat tcttcagttg ctgaactctg taaaagaaca
     4261 cattgcagag ctggaccagg agattgctgt tttctgtcag aaaaatgact aaatagcggc
     4321 tcccacaatg ttcaaatgtg ggagctattt accagcacat cttacattta tgatgagaac
     4381 tcctggcgaa tttgttcccc atgctggtcg agcgttgccg ctcccggcat aacatgcggg
     4441 tccgcgcagc cgctgatttt tatcggatta cccggcatca ttattccccc ggcttcaatc
     4501 aacatatttc tcgcctgagt ttgtggcaat ttaatggcct cagccacact taataacggc
     4561 gcgacgggta caccaacttc atgtattctg gctaaccaaa cttcagctgc ctgcgttttt
     4621 aacgtccgct caatatattg tttaagaata gcctggtttt gtacgcgtaa aatattgctg
     4681 ctaaatcggg gatcattaac cagttccgta agctccagtg cctggcataa cgcagaaaaa
     4741 agcttgtcat taccacaaca aatcgtaatc ggcttatcct gagtattgaa aacatcaaaa
     4801 ggtgccatgt agggatggcg atttcccaga cgttgtggtg acttccctgt cgcgatatat
     4861 gccatcagac catgctccag aaaactcagc gtggcatcaa acatcgctat atcgacatgc
     4921 gcccctctct ggctcttttc gcggccataa agtgcactca ctattccgct gaataaatag
     4981 acaccgccgc atagatccgc aagagatgta ccaacgcgca ctggcggagc atcaggatat
     5041 cctgtttcca tcattatccc gctcattgcc tgaatgatgg tatcgtaggc aggagcatct
     5101 tttagcggac cggtatgtcc gaaacctgac gatgaagcat atatgaggcg cgggttgatt
     5161 tcttgaagcg tttcccatga aaaccccagt ttttccattg tacctgggcg aaaattctca
     5221 gctaatacat cagcttgttt gagcatattt ataaatatac ttttatcgtg atcattcttt
     5281 aaatcaagaa ccacactctc tttgccatga ttaataaaac tgtaatagag tgactgtcca
     5341 tccacatagg gaccaaatgt gcgggtatca tcaccatgac ccggcggctc aactttaatt
     5401 acccttgcgc ccatattaca aagaagttga gttccgaaag gtccattaag gacatgtgtc
     5461 atatcgataa ctaataagcc ttcaaacggc cctttgcttt cattatttgt catttgctta
     5521 tgccttgaaa aaagccgaag gtgaaaagac acttcggcat ttatgtaaaa tcaataacat
     5581 gattaattat gaaaccagtc gactgacata aatccacaag ggagccgtga cgacaaaacc
     5641 cagtacgctc acagccaatg acgctgtacc agtgcgggtg tagacattaa accggctggc
     5701 aataatgatt ccggagaatg ccggcggtaa tgcgcctgcc agtaccatca tctgcaaatg
     5761 ttcgctgttc aaatgacatg ccataccaac gagaagcagc gccagtggca tcagaatcag
     5821 cttcaggaag gtgttataag caatttcggc actgaactcg aatttatgtg cagccagagt
     5881 caaccccgca gcgaataccg ctacccctga gttagccttc gcaatcagat taaaggttgg
     5941 gtcccatgct gccggaattt ttaccccaac caacaccagg atcgttgcca gaacaggtgc
     6001 ccatacaact ggctcctttg ccgcagaaat taatgcgctc agattactat tcttcttacc
     6061 atccgctcct gaggaaggat tcagcaaata cagaccaata ggaatagtaa ttgcgttaac
     6121 aataatagaa ataattgcta ccactaaacc tgttgatacg gaatcaccat aaataggatc
     6181 gagaactgca aaccccagga atccaatggt aggtgaacct gcaattaatg cacatacagc
     6241 tgcttctgca tgggtacgtt taaaaaattt gtagcaaccg aaccaggaga aaaagaaaca
     6301 tccgacaata accacaagtg atacaagggt cagacgagtg tccgcaaaaa tcatttccct
     6361 gtttgcccga gtaatagata caaatagagc cgcaggaagc gcgtagttta ataccagttt
     6421 attaaatgcc cgagcttgat cttctgaaaa agtttctcgt ctgccgctaa aataacccaa
     6481 cagcatgatt acgataatag gcaataaatc gccaataaaa aatgttagca taatagtatt
     6541 tccgataagg gtcccctcac catcgcataa tgagttggca agaggagacg atgtcagtat
     6601 ttaattacca gcgacttgtt ttgggttaag tttggtaata tggccacttt cagttcctgc
     6661 tgcagggtcg atgaccacat taataatggt cggtttgcgc gactgaatac cggtggttaa
     6721 agcatgacga agttcatctg tcgtggtgac gttatagcca acgccacgaa acgcatccat
     6781 taatttgtca taccttgcat ggtgcaacag atccgttggt gatggtgcac cagcgccact
     6841 gagatcaaca ccgtctcctc tgtagatgcc gccattatta aaaataacga tcgtcaccgg
     6901 caggttatat cgacaaatcg tttcaatttc catcccactg aaaccaaaag cactatcacc
     6961 ttcaatggcg acaaccggag aaccagaagt cacgctagca ccgatggcat agcccatacc
     7021 gatgcccatg acaccccagg taccacaatc cagacgacga cgtggtttat acatatcaat
     7081 aatatttcgt gcattatcca gggtatttgc accttcatta actaaataaa tatcctggtt
     7141 ctcgcgcaat acgtcgcgca cagcacttaa tgcattaaag taatttaatg gttgagtatc
     7201 tgtacttaat ttttcatgca ttttttgtgc attttgctgc ttgtggatat ttaaaatatc
     7261 gcgccatacc agtggagtcg taaatgtgtt ttgtttcagt tctgccagca taccttgcat
     7321 actggatgca atatcaccaa cgactggcac agcaatgggg cggttgctgt caatttcctg
     7381 cggttcaata tccagttgaa taaactgtgt atctgccgcc catccttttt taccgtgtgc
     7441 caataaccaa ttcagtcgtg caccaacaag catgacaacg tcagcatttg ccagggcaaa
     7501 cgaacgcgca gctgccgcag aaagtggatg cgtatcttca aggatccctt tcgccataga
     7561 cattggcagg aatggaatct gagtactttc aataaattca cgaagctgtt catcagcttg
     7621 tgaatacgcc gcgcctttgc caaggataat taatggccgt tcagcttttg ctaaaagcga
     7681 aattgcgcta gtgactgact tcgggcatgg caataatgct ggcgatggat tttcaacttt
     7741 aacaatcgtg gttaacgctt cgtctttttc catcgtcgcg gccaggacat ttgctggcaa
     7801 atcaagataa actccgccag ggcgacccga tacagagacc cggatagcgc gtgccaatgc
     7861 aatgccaaga tcctgcggct gattaacgcg aaatgctgct ttggcatacg gttttgccgc
     7921 attcatttgg tccagctctt cataatcacc ttgctgtagg tcgacgatcg cgcggtcgct
     7981 ggagccgcta atcatgatca tcggaaaacc atttaccgtt gcgttggcca atgcggtcaa
     8041 accattgagg aatcctggcg cagaaactgt caggcagatc cccggttttt gggtaagaaa
     8101 accgcttgcc gcagcggcat agcctgccga ctgctcatga cgaaaaccaa tataacgaat
     8161 gccttccgcc tgggcatggc gtgccatatc cgtcacagga atacctacaa caccataaat
     8221 agtgtcaata ttattctgtt ttaatgcttc aacgatgata tgcataccat ctgtcatttg
     8281 aagttgatct gacatgatta ataacctcga aatttttata aattgggagc cagaagcccc
     8341 cgttaatatc agatggcgtg gttttgcttc attgcagcaa tttcatcgtc gctataaccc
     8401 agctcctgca atacagcagc ggtatgttca cctaatagcg gcgcagcttt aatatccggc
     8461 gtaaaggcag agaatttcat tggacaacca acggtcagat attttccacg caacggttgt
     8521 tccacttcaa caacactgcc actttggcgc aaagagggat caagtgaaat ttctttcata
     8581 cttaaaaccg gtgcacaagg aatatcaaac tgagtcaaat aggccaccgc ttcatgttta
     8641 tcaatagtga cagtgtattt ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgt
     8701 gcatgggctg tactgtatgc cggatcggta atccattctg gtttgccgat ggctttacag
     8761 gtgttttccc agttttgctc ctgaatagtg aaataaatat aggcgttagg atcggtttcc
     8821 cagcctttac atttcaggat ccagccaggc tgaccgccac cacctgcatt accaccgcgg
     8881 ggaactgcat caccaaatgt accattcgga tactgcgggt attcttccag ataacccaat
     8941 ttatcgagac gctgctggtc acgtaatttc acgcggcaaa ggttcaatac ggcatcctgc
     9001 attgacatgg tgactcgttg cccacgcccc gttttttcgc gatgcagcaa agcagcaagt
     9061 aaaccgatca gcaaatgcat tccggtgttg ctgtcaccca acgctgcagc gcttaccagc
     9121 ggcggaccat cccaaaaacc cgtagtggat gccgcgccac ccgctgcctg agcaacgttt
     9181 tcataggctt ttacattcac ataaggcgaa cactcatcaa accctttgat cgaaccaaaa
     9241 atcagacgtg gattgatttc ttgaatatgc tcccaggtga agcccatgtg atcaatggcc
     9301 cctggatgaa agttctcgac taagatatca gcttcgcgga tcagcttttc cattacctct
     9361 ttgccttccg ctgttttggt atttaactca atagaacgtt tgttactgtt aagcatggtg
     9421 aagtaaagcg catcgatatc aggaatatct cgcagctggt gacgcgttac gtcaccaacg
     9481 ccgggacgtt caattttaat aacgtcagcg ccaaaccagg ccagcatttg agtacaagat
     9541 gggccagatt gcacaccggt gaaatcgaga actttaattc cttgaagtgg agttgacata
     9601 gcaataccta ttagttaatt aaatatgtgt taatttagcg tttatttaag gcgtaatatc
     9661 ttctataaat aacccgcaaa atatcgtcgt agctgcactc gatattttta aaatcctgta
     9721 tcaagtgctg aaaatcattt catcgccact tccaattcct ttctgcatgg taagtagtga
     9781 ggacgaaaag aagaatatga taaatattga ttcgtggtaa tcagagagag tcgcgacatt
     9841 tgtgatgtta ttcttacaac tgtaagaagc gccggtgatt tacaagtaag ggtatgatta
     9901 cgctttttaa agcgtaccat aaaacataac ataataaatg atttgtgatg acacgggatt
     9961 attgggattt actttgagga agataaaatg cgtgtttata gaaccgcgag cgccaaataa
    10021 ttttatgacc acaacctgtc gtattgtggt cataaaaaat tgtcggctgg agggagttaa
    10081 atcctttacc tcaatatact gcctgtcagc attagttcac aaacagtgct tcgctgtcga
    10141 cgatgattcc atcttccgca cctttaagcg ccaggtttgc ctcgtagtat tgctttttat
    10201 caagcagttt ctgcgcatca gcaagtgcat tacgagtctg tttaagcggc atcagatatt
    10261 ggttttccat tacgccaaca ccggccaggc gaagttcttc cattgcgccc tttttatccc
    10321 ctttcgccat tttttcattg gcaattttta tcgcggcttc tttttcggga gttgctacat
    10381 agctctcaga aattccgaca gatgcattaa tgacaatata ttggtcatca ttgagattgg
    10441 tctttttacc tggcttagcg aactttgccc attcggtact gtcatctgac agcaaagcgg
    10501 aagcttcatt ggtcagttct tttgcctttt ctggatcgcc atgaaacaga gccaggcgcg
    10561 ctacctgcac gtcacgcatc gcatataaac cttgttcaga gatacgctct gctgctgccg
    10621 ttttctgggt ttgctgaact tgctgagttt gctgttgtgc cgctaccggt gcattatcag
    10681 cagcccatac agttgaagat gccagaattg ctgtgaccat cgtggccata attaaacgtt
    10741 tcatacttac ctcattcttt aacagtttta gaaaacgccg ccatcatatt gggtgagcat
    10801 gaaatgagaa atcagagagc aacggtcatt ttgaaagggt tatcttacag ttgtagttat
    10861 tatcgcaaga agtattttag tcttacaatt gtaggaatat gcttcctgtc acagaga
//
LOCUS       DAPGWD010000139        10895 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPGWD010000139 DAPGWD010000000
VERSION     DAPGWD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.140"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            174..877
                     /locus_tag="RHX88_003502"
                     /pseudo
     CDS             174..877
                     /locus_tag="RHX88_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
     gene            complement(1084..1530)
                     /locus_tag="RHX88_003503"
     CDS             complement(1084..1530)
                     /locus_tag="RHX88_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDV2232155.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1622..2281)
                     /locus_tag="RHX88_003504"
     CDS             complement(1622..2281)
                     /locus_tag="RHX88_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV2232156.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2353..2646)
                     /locus_tag="RHX88_003505"
     CDS             complement(2353..2646)
                     /locus_tag="RHX88_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDV2232157.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2888..3289
                     /locus_tag="RHX88_003506"
     CDS             2888..3289
                     /locus_tag="RHX88_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDV2232158.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3409..3777)
                     /locus_tag="RHX88_003507"
     CDS             complement(3409..3777)
                     /locus_tag="RHX88_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232159.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4297..4992
                     /gene="minC"
                     /locus_tag="RHX88_003508"
     CDS             4297..4992
                     /gene="minC"
                     /locus_tag="RHX88_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDV2232160.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5016..5828
                     /gene="minD"
                     /locus_tag="RHX88_003509"
     CDS             5016..5828
                     /gene="minD"
                     /locus_tag="RHX88_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDV2232161.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5832..6098
                     /gene="minE"
                     /locus_tag="RHX88_003510"
     CDS             5832..6098
                     /gene="minE"
                     /locus_tag="RHX88_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDV2232162.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6470..6814)
                     /locus_tag="RHX88_003511"
                     /pseudo
     CDS             complement(6470..6814)
                     /locus_tag="RHX88_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6849..6968)
                     /locus_tag="RHX88_003512"
     CDS             complement(6849..6968)
                     /locus_tag="RHX88_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV2232163.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6929..7114)
                     /locus_tag="RHX88_003513"
     CDS             complement(6929..7114)
                     /locus_tag="RHX88_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232164.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7215..7388
                     /locus_tag="RHX88_003514"
     CDS             7215..7388
                     /locus_tag="RHX88_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232165.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7390..7734
                     /locus_tag="RHX88_003515"
     CDS             7390..7734
                     /locus_tag="RHX88_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDV2232166.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7738..8073
                     /locus_tag="RHX88_003516"
     CDS             7738..8073
                     /locus_tag="RHX88_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDV2232167.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8130..10451)
                     /locus_tag="RHX88_003517"
     CDS             complement(8130..10451)
                     /locus_tag="RHX88_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2232168.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVIDGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10568..10777)
                     /locus_tag="RHX88_003518"
     CDS             complement(10568..10777)
                     /locus_tag="RHX88_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232169.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT         3042 a         2592 c         2382 g         2879 t
ORIGIN      
        1 tagttaaaaa cgcaatcgaa actgcagatg gagcattaga tctttataat aaatatctcg
       61 atcaggtcat cccctggcag acctttgatg aaaccataaa agagttaagt cgctttaaac
      121 aggagtattc acaggcagcc tccgttttag tcggcgatat taaaacctta cttatggata
      181 gccaggataa gtattttgaa gcaacccaaa cagtgtatga atggtgtggt gttgcgacgc
      241 aattgctcgc agcgtatatt tttctatttg atgagtacaa tgagaagaaa gcatccgccc
      301 agaaagacat tctcattaag gtactggatg acggcatcac gaagctgaat gaagcgcaaa
      361 aatccctgct ggtaagctca caaagtttca acaacgcttc cgggaaactg ctggcgttag
      421 atagccagtt aaccaatgat ttttcagaaa aaagcagctt ttccagtcac aggtagataa
      481 aatcaggaag gaagcgtatg ccggtgccgc agccggtgtc gtcgccggtc catttggatt
      541 aatcatttcc tattctattg ctgcgggcgt agttgaaggg aaactgattc cagaattgaa
      601 gaacaagtta aaatctgtgc agaatttctt taccaccctg tctaacacgg ttaaacaagc
      661 gaataaagat atcgatgccg ccaaattgaa attaaccacc gaaatagccg ccatcgggga
      721 gataaaaacg gaaactgaaa caaccagatt ctatgttgat tatgatgatt taatgctttc
      781 tttgctaaaa gaagcggcaa aaaaaatgat taacacctgt aatgagtatc agaaaagaca
      841 cggtaaaaag acactctttg aggtacctga agtctgataa gcgattattc tctccatgta
      901 ctcaaggtat aaggtttatc acatttaaaa acacagacgt tttttaaaga ttgagagtta
      961 cctgttgata ataaggtatc gggtaactct cattatgaca gagcgctttc aaaaacaggg
     1021 ctacgaaatg gcgagcatcc agacgtatat taagaaagcc cccatcaaca tctattaaat
     1081 ttatcactgc gcccagtcag gtttatttaa gatatgatcc tgccagtcaa tcacttctga
     1141 ttctttcact gcgatatgac gcacagagat acgttcacca tgcattgccg ctttcgaacc
     1201 agtaagtagc ggatgccacg caggtaaatc tttaccttcc gccagcaaac gataagcgca
     1261 ggtcattggc agccattcga atgttggcag attttcacgg gttaatttaa tgcagtcggg
     1321 ttcaaactcg aaacgacgtt cgtagttccg acattgacaa gttttaatat tgagctggcg
     1381 acaggcgacg ttagtgaagt agatttcgtc ggtgtcttca tccatcagtt tatgcaggca
     1441 acactgaccg caaccatcac acaacgactc ccattccgca tcgctcattt cgtccagggt
     1501 tttactttgc cagaaaggta catcgctcat caggtgttcc gccattacgt taaaacgcac
     1561 cttataacca gtctggcaca gcgatgcaag ttttgccgcc gctttcaggc ggcaaaaagt
     1621 attacaaaac gcgagttgtc aaagaatgcc catcgaaagt gactgtcagc tcatcaccgc
     1681 tttgcaacgg gccgacgcca tcaggcgtgc ctgtcaacac aacgtcaccg gccttgaggg
     1741 taaaaaactt gctcatataa gcgatcaacg gaacgatttt atggatcatg tccgcagtcg
     1801 taccttgctg gcgttgttcg ccgtttacgc tcaggcccag cgttgtattt tgtggatcgc
     1861 cggtaaattc cgccgcgggg ataaacccgg aaagcggaca agagttatca aacgctttag
     1921 ccttttccca cggctgcccg gctttcttca tttttccctg aacatcacgc aatgtcagat
     1981 caagcgccac gccgtaaccg gcaatggctt tgcggacatg ctcttccgtt gcctgacgca
     2041 gcgtcgcgcc aatcaacacc gccagttcga cttcatgatg aactgaaccg aaatcggatg
     2101 ggatcgccaa tggctgccgc agatcgcaca gtgccgtttc tggtttaata aacagcactg
     2161 gctcttcggg cactgcgctg cccatctctt taatatgttt ggcatagtta ctgccaacac
     2221 agactacttt actcaccgga taatccagca gcgcaccttg ccagttgtga tgttgataca
     2281 taacgttccc ctcaccttat cgtggatatg aaaacccgcg ccgacaaaga taatgccacc
     2341 gccggatatc ggttatttgt tagtgtcgtc tgttttctgc cccatgacgg aaagatgttg
     2401 cttcagcaaa tcttcgggtg gtggcggtaa ctgcaaatag taaccttgct cggttaatgc
     2461 cagtttaacc ttttcaatat cagcattgac cagtttcttg cgcccatcca gcggcagaat
     2521 catcgctaac tgaggctgac caaaaccttt catcagttcc tcaggaacac gcgaaaaatc
     2581 gtcttttttt tcgacatata aataggtctg gtcacgcttg ctgcttcgat aaatcacaca
     2641 aaacatactt ttactcttta agatgccagg ttttggcata agctaacatc aatgaaaccc
     2701 gcgccacaag ccagccagct gtagatttct gcggtttgtt gagtccatgc ccctttataa
     2761 gtaatccccc cggcaaaaaa tgccgcgccg ggattttacc gaacgcagaa aagccactgg
     2821 aattctcccg taagggattg ttatattata acagttcatc gtactcattc tgaacaggag
     2881 actaccaatg aaaatcaaga gcatcagtaa ggctgtattg ctgcttgcac tgctaacctc
     2941 cacgagcttt gctgcgggta agaatgtcaa tgttgagttc agcaaaggcc atagcagcgc
     3001 tcagtactcc ggcgaaataa agggatacga ttacgataca tataccttct atgccaaaaa
     3061 aggtcagaaa gtgcatgtga gtatctctaa tgaaggcgct gatacttacc tcttcgggcc
     3121 aggaatcgat gattcagttg atttatccag atattcacca gaactcgata gtcacggaca
     3181 atactcactc cctgcttctg gcaaatatga gctgcgagtg ctgcaaaccc gaaacgacgc
     3241 ccgtaaaaac aaaactaaaa aatataacgt cgatattcaa attaagtaaa tgtcaaccga
     3301 cggggactct ccccgtcata aacgttaacg ccccactcct ctctgtcgct caagaggtta
     3361 atgacgaata gcagacaatg ccgaatagaa cataatgtaa acaaggcatc actcacgaca
     3421 cataaataac atctttgtgg tgatttgatt gatttttcca ctccgcttac catccacacc
     3481 aaaataacga tcatcacgac ataatttttc tttaacatcg caaaatacac cgttagcgaa
     3541 tgtaaattcg ctgggatcgt agccttgtaa tgattgtaag ttttcagcgg ctttttcgcc
     3601 gagatatttc cttgttaact ctggggaaat gccatctgag ttcacacaaa tatacgggtc
     3661 gcaaacaacg ccgcacgccg gagattgaag tccgccagcg tcatgtccag acgccattga
     3721 tgagaacatc atccccacac ctgacaatcc gatataaaaa atacgcatca tattcatagc
     3781 acatcctgat aatccaaccg tacaacactt atgctcattt catcatcaat ccgtaatcaa
     3841 aaccgctgac ctggacgagt tacgatgacc tgacagaagt atcagacgtc ttctgcggtt
     3901 tctatgatag agcaattctg tggcggatat taagataacg ggtatctgtt ctttaatgat
     3961 gccgcaaaaa atggaaagtt aagaaagcct ggcgtggtga aaaaatacac ttgcctcaat
     4021 actggatatt taaacaggtc tggagatgct gtcacgatta gggtgcggaa aaaggaagcg
     4081 taaataagaa caatcaaaag tacgacggca atgggttgat tgacaagggt attttttaag
     4141 ctatgaatca gcgccattta tcacagaata gacttttact ctgaataaat gggagggtga
     4201 cttgcctcaa tataatccag actataacat gccttatagt cttcggaaca tcatcgcgcg
     4261 ctggcgatga ttaatagcta attgagtaag gccaggatgt caaacacgcc aatcgagctt
     4321 aaaggcagta gcttcacttt atctgtggtt catctgcatg aggcagaacc taaggttatc
     4381 catcaggcgc tggaagacaa aatcgctcag gcccccgcat ttttaaaaca tgcccccgtt
     4441 gtactcaacg tcagtgcact ggaagacccg gtaaactggt cagcgatgca taaggcggtt
     4501 tcggcaaccg gtttgcgggt tattggcgta agtggctgca aagatgcgca acttaaagcc
     4561 gaaattgaaa agatggggct gcctatcctg acggaaggaa aggaaaaagc gccgcgtcca
     4621 gctcccgcac cacaggctcc agcgcaaaat acaacgccgg tcacaaaaac gcgtttaata
     4681 gataccccgg tgcgttccgg tcagcgtatt tatgctccac aatgtgatct gattgttaca
     4741 agccacgtta gcgctggggc cgaattgatt gccgatggga acattcatgt ctatggcatg
     4801 atgcgcggtc gtgcgcttgc aggggcaagt ggtgaccggg aaacgcaaat attttgtacg
     4861 aacctgatgg cggaactggt gtccatcgca ggtgaatact ggctgagtga tcaaatccca
     4921 gcagaatttt atggcaaagc ggcgcgactg cagttagtcg aaaacgcttt gaccgttcaa
     4981 ccgttaaatt gatccctttt taacaaggaa tttctatggc acgcattatt gttgttactt
     5041 cgggcaaagg gggtgttggt aagacaacct ccagcgcggc catcgccact ggtttggccc
     5101 agaagggaaa gaaaactgtc gtgatagatt ttgatatcgg cctgcgtaat ctcgacctga
     5161 ttatgggttg tgaacgccgg gtcgtttacg atttcgtcaa cgtcattcag ggcgatgcaa
     5221 cgctaaatca ggcgttaatt aaagataagc gtactgaaaa tctctatatt cttccggcat
     5281 cgcaaacacg cgataaagat gccctcaccc gtgaaggggt cgccaaagtt cttgatgatc
     5341 tgaaagcgat ggattttgaa tttatcgttt gtgactcccc ggcagggatt gaaaccggtg
     5401 cgttaatggc actctatttt gcagacgaag ccattattac caccaacccg gaagtctcct
     5461 cagtacgcga ctctgaccgt attttaggca ttctggcgtc gaaatcacgc cgcgcagaaa
     5521 atggcgaaga gcctattaaa gagcacctgc tgttaacgcg ctataaccca ggccgcgtaa
     5581 gcagaggtga catgctgagc atggaagatg tgctggagat cctgcgcatc aaactcgtcg
     5641 gcgtgatccc agaggatcaa tcagtattgc gcgcctctaa ccagggtgaa ccggtcattc
     5701 tcgacattaa cgccgatgcg ggtaaagcct acgcagatac cgtagaacgt ctgttgggag
     5761 aagaacgtcc tttccgcttc attgaagaag agaagaaagg cttcctcaaa cgcttgttcg
     5821 gaggataagt tatggcatta ctcgatttct ttctctcgcg gaagaaaaac acagccaaca
     5881 ttgcaaaaga acggctgcag attattgttg ctgaacgccg tcgcagcgat gcagaaccgc
     5941 attatctgcc gcagttgcgt aaagatattc ttgaggtcat ttgtaaatac gtacaaattg
     6001 atcctgagat ggtaaccgta cagcttgagc aaaaagatgg cgatatttct attcttgagc
     6061 tgaacgtgac cttaccggaa gcagaagagc tgaaataagc ccgctgtaaa aacgcattta
     6121 tcttcaaggc agagtcattt ctgccttgag tttttcatcc ctctcatcca cgttgtggta
     6181 aagcggcgag tattcttgct gatactcctc attgctattt ccacctcccc ctttacacct
     6241 taaggctgtt cccacgcaca gaacaaaatg tttatgcctg gttgagtaaa taaccctgtt
     6301 gttagtgtgg atatactggg agatgataac cccgcaattt cattattcat tgttctccca
     6361 taggatgagg tttcccgccg taactggcgg gctttttttg ccccagaatt ttctctttca
     6421 acatcctgta aacgaaaact gcgccgaagc gcagtttaat cgttgatggt cagaacgacc
     6481 agttcacacc agccaccgcg ttccacgggg actccacacc ggcaccatgg ctatacccca
     6541 ccccgaaatg cccgcttaac gtactgctga atgaggcttt aatacctgcc tggtatattc
     6601 cacgtctgcc cgacaaatca ttgacaaaat taccgtcact attcactttc acccgattat
     6661 catcgacaaa ttctttgcgc acagccgcct tcaggcaggg ttcaatttcc ataccgttcc
     6721 ccagacgcat gttgtaactc agcgttgcac ccagttcacg atatatactg cgggtatcga
     6781 ctgatttcga ttccatgcca ttggataaat gatagtagcg tctaagtgac ctaagctctg
     6841 ctgcaacatc agcccgcagg caccagacca ggggattcat cctgaagaga cagcgcaggt
     6901 gtattgtgtt caccgctcac caaagacatc atgatgaaat gatgatatcc cgcataagaa
     6961 agaggcattt tttacacgca gtgcgctgaa gtgtagttgg ataaaaaatc actcttttca
     7021 gataatactg tcgtacgctt atctttcgac aatgaggccg tcggcaaaat aaaatgacta
     7081 atgtaatcgt tgctgatgaa tatcttctgc tcacataaaa atcacacaat aatattgata
     7141 aatcagatta tcttaccttt acaacattgc gttaacagtc aatgacactt cttgaataaa
     7201 aatattgtca atcaatgagt tattcctcat ttaagattat tctcattcac gtaaaaaaca
     7261 taattcctat catcacagcc acgctcattt tgaattatct gaataatagc gagcgttctt
     7321 tagttaagca gatcttaatt gaagacgaaa ttattgtgtg tgcaacttac ttaataccgg
     7381 acatctaagt tgaagaaaaa aatacttgcc ttcgggctta tctcagcatt gttctgctcc
     7441 acgccagcga tggcggatat gaatcgcacc acgaaaggtg cattgctagg cgcaggcgtt
     7501 ggtttactta ctggcaatgg tgttaatggc gtactgaaag gtgcagctgt tggcgctggt
     7561 gttggtgcag taacagaaaa aggccgcgac ggtaaaaatg cgcgtaaagg tgcaaaagta
     7621 ggtgccgccg ttggtgctgt cactggcgtt ctgaccggca atggcctcga aggggcgatt
     7681 aaaggtgccg tgattggcgg tacgggtggt gcaattctgg gtaaaatgaa ataaggaatg
     7741 atgatgaaaa aatttgcttt actggcgggc ctgtttgttt tcgcaccaat gacctgggcg
     7801 caggactaca atattaaaaa cggtttgccg tcagaaacct atattacttg cgcagaagcc
     7861 aacgaaatgg caaaaactga cagcgcacag gttgcagaaa ttgttgcggt aatgggtaat
     7921 gccagcgttg ccagccgtga tttaaaaatt gagcaatctc cggaactgag cgccaaagtg
     7981 gtagaaaaac tgaatcaggt ttgcgctaaa gacccgcaaa tgctgttgat cacggcaatt
     8041 gatgacacca tgcgtgctat cggcaaaaaa taatatttca tatgtaccgc gtcgatgaat
     8101 acgacgcggt tttttagcta acggcgggat cagaacttat atttcaggcc caccatcact
     8161 gcggtgtcat tgtagccatt atcacccagc tgcacgccca cattccccca gacgctggcc
     8221 gctggattca ggttgccatt tacgccaagt ttaatttccc ccaaattact cgccccatct
     8281 tgttcgactt tcacaccgtt cattgagaca gcaaaatctt tactgttatg gatccagttc
     8341 atttcaacaa aaggttcaaa gtcgtcaccg tgcggaccgc tgtttttctc ctgagtacga
     8401 ataaaggtgc ggaaaccaag tcgggtctgg atgttgttat ttgcgtcatt ctcaatacgt
     8461 gagccattgg cttcggtgtg cgcattatga tcgacgccca tccagatagc ctgtgcctgt
     8521 ggctggaata tccacgtgta tttcgccgct tccagaccaa aggatttatt cagggcaaag
     8581 ccgtaacccg cttccagcga gacggttgcg cctttcgcac tataggattc ataggctaat
     8641 tcatcccctt tcaccgagtt tttaaaccag ctatattgcg cccaggcgtc aatgtatgcg
     8701 ccttttttac tgatgtcatc ggcaaaccaa gtggcataca gccctgcgca atagcctctg
     8761 acactccctt tcgaacgata atccgacacg ctggaatgag ttgagttgta gtcgcgggca
     8821 taaccagcca tcactcccaa acgccaactg tcactgtcgg taaaaccacc ggttaacagc
     8881 tcggccccta actgcgagac gtagcgatgc gaggtcgttc tcaactgtcc gttgctgtca
     8941 cgccaggcat tatgcccgcc aatttgacgt agccaaagtc ggcttgaacg ctcctgttca
     9001 gttacaggat cgatgtagcg catttcaccc gcacggtcgt tcagacgcat aacaaaaaga
     9061 gtgttagcgg ctgccaggtt ggcgacgtaa cttccagctt cgaggcgcag gacgggggta
     9121 ggcgcaacag taggcgctga acttcctccg gagtttgttt caccgccgct cgttgcctgg
     9181 aagttagtca gataccagtt tttattggtc gcttcggtat tacctcgggt taatgaatat
     9241 tcgtaggcac cggcgaaaat cctcgaatcc ttaataaatt ccccatttga ttccccctca
     9301 acgctgataa tctctatccc atttaacgtg taagctccgc tgcctcctgc attggtaact
     9361 ttaactcggg ttgttccaga ggtatttcca tcaataacga ctttatccgt agctgaatta
     9421 tcatcaccca attcagttct gagatgcaat acgccattat taccaacgta attttcagtt
     9481 attgttaatc ttgttggttc gacatcccgt ccatctgccc gggaaatata gaccgtacta
     9541 ttatcaaccg tgaggttgct caccgttgag tcagctttaa tatcccaggt actattatct
     9601 gaaagcgaca gataggtatg agtgttaaca tctgttgaaa tattagctga acccgttatt
     9661 tttgaattag ttgcatcgac ggtcaaatga cctttaatat tattgataaa aacatctgca
     9721 gccgatgata cctcactatc cgtaatggtc acgttactcg tctcattttt atggtagagg
     9781 atttcatttg ctgaccgtag tgagctacca gataaaatat ctaccgttgc ttcctgacca
     9841 tttatggaag actccagcac aattccagac ccctgatgcg cgactgcaga tgtatttttc
     9901 agcgtcaccg tgccgccatt ataaatttta gcagtcgcgg cgtcaggacc cgttgtataa
     9961 atttctccct ggttaaatga aactgtcccc tggcgggcag cgatggcatg agcattgtcc
    10021 ccatgcgtgg tgatatcaac atcggttaat gtaacgctgg cattggtata atcaagcgtg
    10081 tcaccagcct tgacgacagt gccattagcg tacgcggcat aactttctgt tccgtgggtt
    10141 gtgatctccc cttttgccat atccagtttt gctatggcat tattttcatt acttcctgta
    10201 tcggtagtac tttcttcatc aagagaagat acacacgcac gcgcgcttcc gggagatgca
    10261 cacgcaagaa cgccgactgc gtgattaccc gcagtaacaa taaatgtatc atctccgtca
    10321 gaaaaagagg gggtaaccga accgtcagag ttgactctta ttatggtgtc agtattcagg
    10381 tttccactcg tttgacttag caaaccaatt gagtaatccc ccgttatgac aatcgtgttt
    10441 ttaccctgca aaataacagc accgccaaat tcacttacga ttgcgctagc tgttaatgct
    10501 gtgcttagag gactattgtc agaaggatat aaaccgcccg ttgttacaat gacgccattg
    10561 ttaatggtta acttgctacc ctctcctgca acggataccc gtaacttcca ttacccgttg
    10621 ttataactag cgcattatca atatctttta cttttccatc ttttattgcc acagcatgtt
    10681 tatattcatc gtgctgaata aactgttcag catatgcatt acccaccgcc agagcaataa
    10741 gtcccaaact aaacccggag agttttttca gtttcataga ttaaccatcc attcattgga
    10801 ttaacaaaca ttcctggtca aataacctat gcgttcagat taccaaaacg gagggtaaag
    10861 acaaaaacaa aagcacaaaa aatttaaata gataa
//
LOCUS       DAPGWD010000140        10870 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPGWD010000140 DAPGWD010000000
VERSION     DAPGWD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.141"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(104..1768)
                     /gene="glnS"
                     /locus_tag="RHX88_003519"
     CDS             complement(104..1768)
                     /gene="glnS"
                     /locus_tag="RHX88_003519"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDV2232170.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1971..3917)
                     /gene="nagE"
                     /locus_tag="RHX88_003520"
     CDS             complement(1971..3917)
                     /gene="nagE"
                     /locus_tag="RHX88_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDV2232171.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4250..5050
                     /gene="nagB"
                     /locus_tag="RHX88_003521"
     CDS             4250..5050
                     /gene="nagB"
                     /locus_tag="RHX88_003521"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV2232172.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5110..6258
                     /gene="nagA"
                     /locus_tag="RHX88_003522"
     CDS             5110..6258
                     /gene="nagA"
                     /locus_tag="RHX88_003522"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV2232173.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6267..7487
                     /gene="nagC"
                     /locus_tag="RHX88_003523"
                     /gene_synonym="nagR"
     CDS             6267..7487
                     /gene="nagC"
                     /locus_tag="RHX88_003523"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDV2232174.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7535..8287
                     /gene="nagD"
                     /locus_tag="RHX88_003524"
     CDS             7535..8287
                     /gene="nagD"
                     /locus_tag="RHX88_003524"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDV2232175.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8544..10208
                     /gene="asnB"
                     /locus_tag="RHX88_003525"
     CDS             8544..10208
                     /gene="asnB"
                     /locus_tag="RHX88_003525"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDV2232176.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNSKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10589..10665
                     /locus_tag="RHX88_003526"
     tRNA            10589..10665
                     /locus_tag="RHX88_003526"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10623..10625,aa:Met,seq:cat)
     gene            10675..10759
                     /locus_tag="RHX88_003527"
     tRNA            10675..10759
                     /locus_tag="RHX88_003527"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10709..10711,aa:Leu,seq:tag)
     gene            10783..10857
                     /locus_tag="RHX88_003528"
     tRNA            10783..10857
                     /locus_tag="RHX88_003528"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10815..10817,aa:Gln,seq:ttg)
BASE COUNT         2692 a         2811 c         2779 g         2588 t
ORIGIN      
        1 aagaaaagca gaaaaaacgc cgcagagtgc ggcgtttggg attgtttgct atttcttgcc
       61 ggatgcgacg taaatgcctc acccggcctg gtagaactta aaattactcg cctactttcg
      121 cccaggtatc acgcagccca acggtgcggt taaataccgg tttttccgct gtggaatggc
      181 ggctgtcgag gcagaagtaa ccttcacgct caaactggaa tgctttaccc gcaaccgcat
      241 ctttcagcga cggttcagca aagccctgtt tgatcaccag cgattccggg ttaatcaccg
      301 acaggaaatc atccgcagca ccagggttcg gcacgctgaa cagacggtcg tacagacgga
      361 tttcaaccgg cagcgcatgt gccgcgctca cccagtgaat aacacctttg actttacgac
      421 catctgccgg atctttgctt aaggtgtcgg cgtcataagt acagaagatg gtggtgatat
      481 tgccttcggc atctttctcc acgcgttcag ccttaatgac gtaagcatta cgcagacgca
      541 cttctttacc cagcaccaga cgtttgtact gcttgttagc ttcttcgcgg aaatcggcgc
      601 gatcgatcca aatctcaccg ctaaacggca cctgacgact ccccatttcc ggtttgttcg
      661 gatggttcgg catggtgacc atttcgcctt cgccctgata gttttcgata accagtttca
      721 ccggatcgat aaccgccatt gcgcgcggcg cattttcgtt gagatcttca cggatgcagg
      781 attccagcga tgccatctca atggtgttgt cctgcttggt cacgccgatg cgtttgcaga
      841 actcacgaat agaagcagca gtgtaaccac gacgacgcag accggaaatg gtcggcatac
      901 gcgggtcatc ccagccttca acgtgcttgt cggtcaccag caggttcagc ttacgcttgg
      961 acatcacagt gtattccaga ttcaggcgcg agaattcgta ctggcgcggg tgaacaggaa
     1021 tggtgatgtt gtccagcacc cagtcgtaca ggcgacggtt gtcctggaac tcaagcgtac
     1081 acagagagtg cgtaatacct tccagggcat cgctgatgca gtgggtgaag tcgtacatcg
     1141 ggtagatgca ccacttgttg ccagtctggt ggtgttcagc aaatttaata cggtacagca
     1201 ccggatcgcg catcacgata aacggtgaag ccatgtcgat tttcgcacgc aggcaggctt
     1261 taccttcttc aaaaccaccg gcacgcattt tttcgaacag cgccaggttc tcttcaacgc
     1321 tgcggtcgcg gtacgggctg tttttacccg gttgcgtcag ggtgccgcgg tattcgcgga
     1381 tctgttccgg cgtcagttca tcaacgtacg ccaggccttt attgatcagt tcgatcgcat
     1441 aggcgtggag ctgatcaaaa taatcggagg agtaacggac gttaccagac cagtgaaaac
     1501 ctaaccactc tacgtcgttt ttgatcgact caacatactc gatatcttct tttaccgggt
     1561 tagtgtcgtc gaaacgcagg ttgcactggc ctttatagtc ctgggcgatc ccgaagttca
     1621 ggcagataga tttcgcatgg ccaatatgca gatagccatt cggctccggc gggaaacggg
     1681 tgtgtactgt ggtgtgctta ccactggcca gatcttcatc gatgatctga cggataaagt
     1741 tagtcgggcg ggcttctgcc tcactcatcg tggattcctc aaagcgtaaa caacgtataa
     1801 cggcgtatga tcttataagc gggacaggct gacaactgtt agttttttaa aattgataat
     1861 ctaacaagga ttatggggat gattgatgaa aaaaaaagcg gcagagaaga tctccaccgc
     1921 ttgtcattgt tggatgcgac gctcaagcgt cgcatcaggc ataaagcaga ttactttttg
     1981 atttcataca gcggtgtttg acccgccaca acatggccct gagctttaat gatcaagcca
     2041 ctgaaatcgt cgatattgct gcaaaccacc gggctaatca tcgagcgggc gttagcgttc
     2101 aggtaatcca gatccatttc cagaatcggt tgccctgcgc ttacctgcgc accctcttcc
     2161 accagacgtt taaagccttt accttccagc gctacggtgt cgatacccat atggacgacg
     2221 atctccgcgc ctttttcggt ttccaggcag aacgcgtggt tggtgttgaa gattttcacg
     2281 attgtccctg cggctggtga tacgacgatt ttatctgtcg gtttcaccgc cacaccgtca
     2341 cccaccgctt tgctggcgaa tgcttcgtca ggaacctgat ccagtgccac gacatcaccg
     2401 gtaatcggcg ataccagctc cgcgatagat accgcgtttg gtacagcctg cggttttgct
     2461 acaggcgcgg cagttgccgg agttgcttca gctgacgcag cggctaccgg accacgggca
     2521 acgactttct tcatcgcatc gccgatggat tctgctttcg cgccaacaat cacctgaata
     2581 gtctgtttgt tcagtttcac tacaccagaa gcacccagac gtttacacat cgtatcgttg
     2641 acgcgggcag agtcagccac ggtcaggcgc agacgggtga tacaggcgtc aattgctttc
     2701 aggttgtcag tgccgccaac cgcagcaata tagttggtcg ccagttgatt gaggccttct
     2761 tcagtgttgc tgttggcttc ttcagtaacg atctcgtctt ctttatcttc acgacccggc
     2821 gttttcaggt tgaacatgcg gataaccaaa ctgaacacca cgaagtagat agcgaagaag
     2881 acaacaccca taaccagcag catccagacg ttctggctgg cggccggcag gttatacatc
     2941 aacgcgtagt cgatagcccc cgcagagaaa gagaagccag catggatacc cagcagtgtt
     3001 gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga ggtacagcag cggtgcaagg
     3061 aacatgaaca ggaattccag cggctcagtc acaccggtca ggaacgcagt aacagcaaca
     3121 gaaagcagca tcccgccaac catcggacga cgctctttcg gtgctgcgaa gtacatcgcc
     3181 agcgccgcac ccggcagacc gaacatcata atcgggaaga agccggacat gaacatcccc
     3241 gcggtgccgt caccggcata gaagcggttg atatcaccgt ggaaaaccgt acccgccgcg
     3301 ttggtgaatt caccaatctg gaaccaggcg atggtgttca gcacctgatg cagaccggtt
     3361 gggatcagca gacggttgat gaaaccaaag ataccggaac ccagcgcgcc cgcagaaacg
     3421 atccactcgc ctcctgcatg gatagcgtgc tgtaccggcg gccagacgta accaaaaatg
     3481 gccgccagca ccaggcagaa gaagccggtg gcaatcggca caaagcgttt gccgccgaag
     3541 aaactcagga agtccggcag tttaatatcg gaccaacggt tataggctgc gccaccaacc
     3601 agaccggtaa tgatacccgc cagtacaccc atgttaattt ctgggttgat ggtcaccatc
     3661 gctttggtta acacaaagta acctaccgca cccgccagcg ccgccgcacc tgcgctgtct
     3721 ttcgaccagc tggatgccac accgatggcg aagattaatg cgaggttatc aaaaatcgca
     3781 ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg gctgaccgaa tcgcagcaac
     3841 agtgccgcca ccggcagcac cgcgataggg agctgtaacg ccctaccgag tcgctggaaa
     3901 aaacctaaaa tattcatctt attcccccta cgagaaccct atttggctcg tttaaagccg
     3961 tatttttatt ttgctgcaaa ttgtactgcc gatgttctgt aatcagattg ttagatcatc
     4021 tgctacagag tgtgtgaaaa tttaattcgt atcgcaaatt aaacgcgtgt cttttgtgag
     4081 ttttgtcacc aaatatcgtt atcatcactc ccttttactg gctaaagcag aaaacttatt
     4141 ttatcattca aaaaatcagg tcggattgac gcctgtctgc gcaaatccag gttacgctta
     4201 aagatgccta atccgccaac ggcttacatt ttacttattg aggtgaataa tgagactgat
     4261 ccccctgact accgctgaac aggtcggcaa atgggctgct cgccatatcg tcaatcgtat
     4321 caatgcgttc aaaccgactg ccgatcgtcc gtttgttctg ggcctgccga ctggcggcac
     4381 gccgatgacc acctataaag cgttagtcga aatgcataaa gcaggccagg tcagctttaa
     4441 gcacgttgtc accttcaaca tggacgaata tgtcggtctg ccgaaagagc atccggaaag
     4501 ctactacagc tttatgcacc gtaatttctt cgatcacgtt gatattccag cagaaaacat
     4561 caaccttctc aacggcaacg ccccggatat cgacgccgaa tgccgccagt atgaagaaaa
     4621 aatccgttct tacggaaaaa ttcatctgtt tatgggcggt gtaggtaacg acggtcatat
     4681 tgcatttaac gaaccggcgt cttctctggc ttctcgtact cgtatcaaaa ccttgactca
     4741 tgacactcgc gtcgcgaact ctcgtttctt tgataacgat gtcaatcagg tgccaaaata
     4801 tgccctgact gtcggtgttg gtacattgct ggatgccgaa gaagtgatga ttctggtgct
     4861 gggtagccag aaagcactgg cactgcaggc tgcagttgaa ggttgcgtga accacatgtg
     4921 gaccatcagc tgtctgcaac tgcatccaaa agcgatcatg gtgtgcgatg aaccttccac
     4981 catggagctg aaagttaaga ctttaagata tttcaatgaa ttagaagcag aaaatatcaa
     5041 aggtctgtaa ttgttatccc tgccctggct ccttgctcag ggcaatattt tttaaaatcg
     5101 ggggtcagaa tgtatgcatt aacccagggc cggatcttta ccggccacga atttcttgat
     5161 gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg tctgtccggt agcggaactg
     5221 ccgccagaga tcgaacaacg ttcactgaac ggggccattc tctcccccgg ttttatcgat
     5281 gtgcagttaa acggctgcgg cggcgtacag tttaacgaca ccgctgaagc ggtcagcgtg
     5341 gaaacgctgg aaatcatgca gaaagccaat gagaaatcag gctgtactaa ctatctgccg
     5401 acgcttatca ccaccagcga tgagctgatg aaacagggcg tgcgcgttat gcgcgagtac
     5461 ctggcaagac atccgaatca ggcgttaggt ctgcatctgg aaggtccgtg gctgaatctg
     5521 gtaaaaaaag gcacccataa tccgaatttt gtgcgtaagc ctgatgccgc gctggtcgat
     5581 ttcctgtgtg aaaacgccga cgtcattacc aaagtgaccc tggcaccgga aatggttcct
     5641 gcggaagtca tcagcaaact ggcaaatgcc gggattgtgg tttctgccgg tcactccaac
     5701 gcgacgttga aagaagcaaa agccggtttc cgcgcgggga ttacctttgc cacccatctg
     5761 tacaacgcga tgccgtatat taccggtcgt gaaccgggcc tggcgggcgc gatcctcgac
     5821 gaagctgaca tttattgcgg tattatcgct gatggcctgc atgttgatta cgccaacatt
     5881 cgtaacgcta aacgccttaa aggcgacaaa ctgtgtctgg ttaccgacgc caccgcgcca
     5941 gcaggtgcca acattgaaca gttcattttt gcgggtaaaa caatatacta ccgtaacgga
     6001 ctttgtgtgg atgagaacgg tacgttaagc ggttcatcct taaccatgat tgaaggcgtg
     6061 cgtaatctgg tcgaacattg cggtatcgca ctggatgaag tgctgcgtat ggcgacgctc
     6121 tatccggcgc gtgcgattgg cgttgaaaaa cgtctcggca cgctcgccgc aggtaaagta
     6181 gccaacctga ccgcattcac acctgatttt aaaatcacca agaccatcgt taacggtaac
     6241 gaggtcgtaa ctcaataaga gaaagtatga caccaggcgg acaagctcag ataggtaatg
     6301 ttgatctcgt aaaacagctt aacagcgcgg cggtttatcg cctgattgac cagtacgggc
     6361 caatctcgcg gattcagatt gccgagcaaa gccagcttgc ccccgccagc gtaaccaaaa
     6421 ttacgcgtca gcttatcgaa cgcgggctga tcaaagaagt tgatcagcag gcctccaccg
     6481 ggggccgccg cgctatctcc atcgtcaccg aaacccgcaa tttccacgca atcggcgtac
     6541 ggcttggtcg tcatgacgcc accatcactc tgtttgatct cagcagcaaa gtgctggcag
     6601 aagaacatta cccgctgccg gaacgtaccc agcagacgct ggaacatgcc ctgctgaatg
     6661 ccattgctca gtttattgat agctaccagc gcaagctgcg cgagctgatc gcgatttcgg
     6721 tgatcctgcc agggcttgtt gacccggaca gcggcaaaat tcattacatg ccgcatattc
     6781 aggtggaaaa ctgggggctg gtagaagcac tggaagagcg ttttaaagtg acctgttttg
     6841 tcggccacga tatccgtagt ctggcgctgg cagagcacta cttcggtgca agtcaggatt
     6901 gcgaagactc cattttagtg cgtgtccatc gcggaaccgg ggccgggatt atctctaacg
     6961 ggcgcatttt tatcggccgc aacggtaacg tcggtgaaat tggccatatt caggtcgaac
     7021 cgctgggtga acgctgccac tgcggcaact ttggctgcct ggaaactatc gctgccaacg
     7081 ctgctattga acaacgggtg ttgaatctgt taaagcaggg ctaccagagc cgcgtgccgc
     7141 tggacgactg caccatcaaa actatctgca aagccgcgaa caaaggcgat agcctggcgt
     7201 cggaagtgat tgagtatgtc ggtcgtcatc tgggcaaaac catcgccatt gcgatcaact
     7261 tatttaatcc gcaaaaaatt gttattgccg gtgaaatcac cgaagccgat aaagtgctgc
     7321 tccctgctat tgaaagctgc attaataccc aggcgctgaa ggcgtttcgt actaatctgc
     7381 cggtggtacg ttctgagctg gatcaccgct cggcaatcgg cgcttttgcg ctggtaaaac
     7441 gcgccatgct caacggtatt ttgctccagc atttgctgga aaattaatgt gcttttatag
     7501 tggcgcttat tgttgtcaat attctgggta gtccatgacc attaaaaatg taatttgcga
     7561 tatcgacggc gtgctgatgc acgataacgt cgccgtaccg ggtgcagcgg aatttttgca
     7621 cgggattatg gataaaggcc tgccgctggt gttgctgacc aactatcctt cgcagaccgg
     7681 gcaggatctg gcgaaccgct ttgccactgc cggtgtcgat gtgccggata gcgtgtttta
     7741 tacctctgcg atggcgaccg ccgatttcct gcgtcgccag gaaggcaaga aagcgtatgt
     7801 ggtgggcgaa ggcgcactga ttcatgaact gtacaaagcc ggtttcacta ttaccgatgt
     7861 gaaccctgat tttgtgattg ttggcgaaac gcgttcctac aactgggaca tgatgcataa
     7921 agcggcctat ttcgtcgcta acggtgcacg ctttatcgcc accaacccgg atactcacgg
     7981 gcgcggtttt tatccggcct gtggcgcgtt gtgtgcaggg attgagaaaa tctccgggcg
     8041 caaaccgttc tatgttggta agcccagccc gtggatcatc cgcgcggcat taaacaaaat
     8101 gcaggcgcat tcggaagaaa cggtgattgt cggcgataac ttgcgcaccg acattctggc
     8161 gggcttccag gcgggtctgg agacgattct ggtgctttct ggtgtttcat cgctcgacga
     8221 tatcgacagt atgccgttcc gcccaagctg gatttacccg tcggtcgctg aaatcgacgt
     8281 tatctgaata gcaaccacgt ctcagggcgt ggtttcttat ttttacccat cattaaaatc
     8341 atcccattca ataaaactcg ccaaaaatta tcgatccatc aataaataca tcaccacacc
     8401 ctcccctttt tcacaaatca gcaatcacca ttacgttttt tatttttgca ctgccaaatc
     8461 gcttgcgcac ggtgctgctt tgcggcattt ttttaaacaa gcaaacacaa caagcaacaa
     8521 ataccaggtt aacggagaag gttatgtgtt caatttttgg cgtattcgat atcaaaacag
     8581 acgcagttga gctgcgtaag aaagcactcg agctgtcacg cctgatgcgt catcgtggcc
     8641 cggactggtc cggtatttat gccagcgata acgccattct cgcccacgaa cgtctgtcaa
     8701 ttgttgacgt taacgcgggg gcgcaacctc tctacaacca acaaaaaact catgtgctgg
     8761 cggtaaacgg tgaaatctac aaccaccagg cactgcgcgc tgaatatggc gatcgttatc
     8821 agttccagac agggtctgac tgtgaagtga tcctcgcgct gtatcaggaa aaagggccgg
     8881 aatttctcga cgacttgcag ggcatgtttg cctttgccct gtacgacagc gaaaaagatg
     8941 catatctgat tggtcgcgac catctgggga tcatcccact gtatatgggc tatgacgagc
     9001 acggtcagct gtatgtggcc tcagaaatga aagccctggt gccagtttgc cgcacgatta
     9061 aagagttccc ggcggggagc tatttgtgga gtcaggacgg cgaaatccgt tcttattatc
     9121 atcgcgactg gttcgacttt gatgcagtga aagataacgt gaccgacaaa aacgagctgc
     9181 gtcaggcact ggaagattcc gttaaaagcc atctgatgtc tgacgttcct tacggtgtgc
     9241 tgctctcggg tggtctggat tcctcaatta tttccgcgat caccaagaaa tacgccgccc
     9301 gtcgcgtgga agatcaggaa cgctctgaag cctggtggcc gcaattacac tcctttgctg
     9361 taggtctgcc gggttcaccg gatctgaaag cagcccagga agtggcaaac catctgggca
     9421 cggtgcatca cgaaattcac ttcactgtac aggaaggtct ggatgccatc cgcgacgtga
     9481 tttaccacat cgaaacctat gatgtgacaa ctattcgcgc ttcaacaccg atgtatttaa
     9541 tgtcgcgtaa gatcaaggca atgggcatta aaatggtgct gtccggtgaa ggttctgatg
     9601 aagtgtttgg cggttatctt tacttccaca aagcaccgaa tgccaaagaa ctgcatgaag
     9661 agacggtgcg taaactgctg gccctgcata tgtatgactg cgcgcgcgcc aacaaagcga
     9721 tgtcagcctg gggcgtggaa gcacgcgttc cgttcctcga caaaaaattc cttgatgtgg
     9781 cgatgcgcat taacccgcag gataaaatgt gcggtaacag caaaatggaa aaacacatcc
     9841 tgcgtgaatg ttttgagtca tacctgcccg caagcgtggc ctggcggcag aaagagcagt
     9901 tctccgatgg cgtcggttac agttggatcg acaccctgaa agaagtggcg gcgcagcagg
     9961 tttctgatca gcaactggaa actgcccgct tccgcttccc gtacaacacg ccaacctcaa
    10021 aagaagcgta tctgtaccgg gagatctttg aagaactgtt cccgcttccg agcgccgctg
    10081 agtgcgtgcc tggcggtcct tccgtcgcgt gttcttccgc taaagcgatt gagtgggatg
    10141 aggcgttcaa gaaaatggac gatccgtctg gtcgtgcggt tggcgttcac cagtcggcgt
    10201 ataagtaaga aatgcgaaac agccccggtg aaatccgggg ctgtttcagt tattgaggtg
    10261 cttgttaatt ctgatatgca gtaaggaaat gttccgggtt tgccacatca ctgtttgaca
    10321 actcatcact gtaggccagt atttcataga ttaatttccc accactattg ttgaattccg
    10381 aatttcatcc tgtacgacaa aagaatggct gtagggtggg gaaaaaacac aaagtaaaca
    10441 ataattgacg aatatagcgc cacgctgttc gcaacctaac caaacagtca ctttcgagca
    10501 attttccttg aaaaagaggt tgacgctgca aggctctata cgcataatgc gccccgcaac
    10561 gccgataagg tatcgcgaaa aaaaagatgg ctacgtagct cagttggtta gagcacatca
    10621 ctcataatga tggggtcaca ggttcgaatc ccgtcgtagc caccatcttt ttttgcggga
    10681 gtggcgaaat tggtagacgc accagattta ggttctggcg ccgcaaggtg tgcgagttca
    10741 agtctcgcct cccgcaccat tcaccagaaa gcgttgtacg gatggggtat cgccaagcgg
    10801 taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc ccagccatcg
    10861 aagaaacaat
//
LOCUS       DAPGWD010000141        10859 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPGWD010000141 DAPGWD010000000
VERSION     DAPGWD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.142"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(234..1286)
                     /gene="fbaB"
                     /locus_tag="RHX88_003529"
     CDS             complement(234..1286)
                     /gene="fbaB"
                     /locus_tag="RHX88_003529"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDV2232177.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1543..2820
                     /locus_tag="RHX88_003530"
     CDS             1543..2820
                     /locus_tag="RHX88_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HDV2232178.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2817..3821
                     /locus_tag="RHX88_003531"
     CDS             2817..3821
                     /locus_tag="RHX88_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDV2232179.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            3818..4783
                     /locus_tag="RHX88_003532"
     CDS             3818..4783
                     /locus_tag="RHX88_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV2232180.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4757..5503)
                     /locus_tag="RHX88_003533"
     CDS             complement(4757..5503)
                     /locus_tag="RHX88_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2232181.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5555..6373)
                     /locus_tag="RHX88_003534"
     CDS             complement(5555..6373)
                     /locus_tag="RHX88_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDV2232182.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(6438..7238)
                     /gene="thiD"
                     /locus_tag="RHX88_003535"
     CDS             complement(6438..7238)
                     /gene="thiD"
                     /locus_tag="RHX88_003535"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDV2232183.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7235..8023)
                     /gene="thiM"
                     /locus_tag="RHX88_003536"
     CDS             complement(7235..8023)
                     /gene="thiM"
                     /locus_tag="RHX88_003536"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDV2232184.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8058..8154)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8246..8518)
                     /gene="rcnR"
                     /locus_tag="RHX88_003537"
     CDS             complement(8246..8518)
                     /gene="rcnR"
                     /locus_tag="RHX88_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDV2232185.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8639..9463
                     /gene="rcnA"
                     /locus_tag="RHX88_003538"
     CDS             8639..9463
                     /gene="rcnA"
                     /locus_tag="RHX88_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDV2232186.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLITVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9682..10020
                     /gene="rcnB"
                     /locus_tag="RHX88_003539"
     CDS             9682..10020
                     /gene="rcnB"
                     /locus_tag="RHX88_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDV2232187.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10102..>10859)
                     /locus_tag="RHX88_003540"
     CDS             complement(10102..>10859)
                     /locus_tag="RHX88_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDV2232188.1"
                     /translation="ITLSNNQTSFTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVAT
                     LSSASSSTRGSAAVQFLLCLLGGKSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTT
                     CKPDDLLITLDSIPVSQLPATGNKATINSKQGDIILRCKNLLGQQNKTSRKMQVYLSS
                     SDLLTNSNTILKGAEDNGVGFILESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTT
                     VSIPITASYYVYDTNKVKSGALEATALINVKYD"
BASE COUNT         2531 a         2761 c         2745 g         2822 t
ORIGIN      
        1 caaaaacgac aatgtgaaaa ttgttcgagg agtaaaaggc aaaaaaaaac ggcctcccga
       61 tagggaagcc gtagcaaagt gcgcgtgttt ttatgccgga tgcggtgtaa acatacgtgg
      121 cagtgctatg taggcatgat aagacgcttc cgcgtcgcat caggcatagg ttgccggatg
      181 cagtgtaaac gccttatccg tcctacgggg tggtgctgtt cgtatgaggc gtatcaggca
      241 atagtaattt tgctatcgag ataaacgtcc tgcacggcgt taatcagttt cacgccgtca
      301 gccatcgatt tcttgaacgc cttacgtcca agaatcagcc ccattccgcc agcgcgtttg
      361 ttgataaccg cagtacgcac tgcatcactg aggtcagttt caccgcctgc agcaccgccg
      421 gagtttatca acccagcccg gcccatatag cagttagcta actgataacg caccagatca
      481 atcgggtttt cgctggttaa cttgctgtac acgcgatcgt cggtataacc gtaattaatt
      541 gctttatagc cgccgttatt ttccgccatt ttttgtttga cgatatctgc accgatggtt
      601 gccgccagat ggtttgcctg accggtcagg tcggcggaaa catggtaatc aacgccatct
      661 ttcttaaagg cggagttacg caaataggcc cacagcactg tcaccatacc cagctcgtgc
      721 gcacgttcaa aagccgcaga aatttcttca atctggcgac gtgactcttc cgagccaaaa
      781 tagatagtcg caccaaccgc aaccgcgccc atgttgaacg cctgctccac gctggcatac
      841 agcgtttgat cgtaggtatt cgggtaactt agcgtctcgt tgtgattaag tttgacgagg
      901 aatggaatgc gatgcgcata acgccgcgat accgacgcca gcacgccgta agttgacgcc
      961 acacagttac agcccgcttc gatcgccagt tcaacaatgt ttttcgggtc aaagtagagc
     1021 gggttagcag caaatgaagc tccggcagag tgctcaacgc cctggtcaac cggcagaata
     1081 gaaagatatc ctgtgccagc cagacgcccg gtgttgtaca acgtctgcat attacgtaac
     1141 accgctggcg ggcgattatt gtcaatcatt acgcggtcta cgtagtcatg tccggggaga
     1201 taaagctggt cagaaggaat tgtcatacaa cggtgctgta aaaggttgtc ggcgtctttg
     1261 ccaagcaact gcgcaatatc tgtcattact atgctcccgt aaattccgat tggatatcgg
     1321 ctatggattg tcctggcccg ccttttgcgg gcaatcataa tcctggtcgt tacgggcgag
     1381 tttttccatc ttttggatgt tttttcagcg ttttctgttg gctcgattca tcagaaaaaa
     1441 tgttagcgcg gtcaagtttt caccgcaaag gtatttaaaa ggtattatta agtggtattg
     1501 tcatcgcgta ccttacatta cctgtcatga aggaattaaa agatgaaaac aacagcaaag
     1561 ctgtcgttca tgatgtttgt tgaatggttt atctggggcg cgtggtttgt gccattgtgg
     1621 ttgtggttaa gtaaaagcgg ttttagtgcc ggagaaattg gctggtcgta tgcctgtacc
     1681 gccattgcgg cgatcctgtc gccaattctg gttggctcca tcactgaccg ctttttctcg
     1741 gcgcaaaaag tgctggcggt attgatgttc gcaggcgcgc tgctgatgta tttcgctgcg
     1801 caacagacca cttttgccgg gttctttccg ttactgctgg cttactctct aacctatatg
     1861 ccgaccattg cgctgactaa cagcatcgct tttgccaacg tgccggatgt ggagcgtgat
     1921 ttcccgcgca ttcgtgtgat gggcactatc ggctggattg cctccggtct ggcatgtggt
     1981 ttcttgccgc aaatactggg gtatgccgat atctcaccga ctaacatccc gctgctgatt
     2041 accgccggaa gttctgctct gctcggtgtg tttgcgtttt tcctgcccga cacgccacca
     2101 aaaagcaccg gcaaaatgga tattaaagtc atgctcggcc tggatgcgct gatcctgctg
     2161 cgcgataaaa acttcctcgt ctttttcttc tgttcattcc tgtttgcgat gccactggcg
     2221 ttctattaca tctttgccaa cggttatctg accgaagtcg gcatgaaaaa tgccaccggc
     2281 tggatgacgc tcggccagtt ctctgaaatc ttctttatgc tggcattgcc gtttttcact
     2341 aaacgctttg gtatcaaaaa ggtattattg cttggtctgg tcaccgctgc gatccgctat
     2401 ggcttcttta tttacggtag tgcggatgaa tatttcacct acgcgttact gttcctcggt
     2461 attttgcttc acggcgtaag ttacgatttt tactacgtta ccgcttacat ctatgtcgat
     2521 aaaaaagccc ccgtgcatat gcgtaccgcc gcgcaggggc tgatcacgct ctgctgccag
     2581 ggcttcggca gtttgctcgg ctatcgtctt ggcggtgtga tgatggaaaa gatgttcgct
     2641 tatcaggaac cggtaaacgg actgactttc aactggtccg ggatgtggac ttttggcgcg
     2701 gtgatgattg ccattatcgc cgtgctgttc atgatttttt tccgcgaatc cgacaacgaa
     2761 attacggcta tcaaggtcga tgatcgcgat attgcgttga cacaagggga agttaaatga
     2821 aaacagaacg tattctcggt gctctttatg ggcaggcgtt aggggatgcg atggggatgc
     2881 cctccgagct ttggccacgc agccgcgtta aagcacactt tggctggatt gaccgttttc
     2941 ttcctggacc aaaggagaat aacgcggcct gttattttaa ccgcgccgaa ttcaccgacg
     3001 atacctcgat ggcgctgtgt ctggcggatg cgttactgga acgtgaaggc aagatcgatc
     3061 cggatctgat tgggcgtaat attctcgact gggcgctgcg tttcgacgcc tttaacaaaa
     3121 acgtactagg tccgacctcg aagattgcgc ttaacgccat tcgcgacggt aaacccgttg
     3181 ctgaactgga aaacaacggc gtgaccaacg gcgcagcgat gcgcgtctcg ccattaggtt
     3241 gtttgcttcc ggcgcgtgat gttgattcct ttattgatga tgtggcgctg gcgtcgagcc
     3301 cgacccataa atccgatctg gcagttgcag gtgcggtagt catcgcatgg gcgatttctc
     3361 gtgccattga cggagaaagc tggtcagcga ttgttgattc cctgccttca attgcgcgac
     3421 atgcacagca aaaacgcatc actaccttca gcgcctcact ggcagcacgt ctggagattg
     3481 cgctgaaaat tgtgcgcaat gccgacggca ccgaatccgc cagcgaacag ctttaccagg
     3541 tcgttggcgc aggtaccagc actattgagt ccgttccgtg cgccattgcg ctggttgaac
     3601 tggcacaaac cgacccgaat cgctgcgccg tcctgtgcgc taaccttggc ggcgacacag
     3661 acaccatcgg cgctatggcg acggcgattt gcggcgcgtt gcatggcgtt aacgctatcg
     3721 atcctgcatt aaaggcggaa ctggatgcgg taaatcagct tgatttcaac cgctatgcca
     3781 cagcgctggc gaaatatcgt caacaacggg aggcggtatg agcggcgctc gattacacac
     3841 gctgctgcct gaattaacct cgcgtcagcc ggtgatggtc gtcggcgcgg cggtcattga
     3901 tgtgatcgcc gacgcttatg ccctcccctg gcgtgggtgc gatatcgaac tgaagcagca
     3961 gagcgttaac gttggcggct gcgcactgaa tattgccgtg gcgttaaagc gcctcggcat
     4021 cgaagcgggt aatgccttgc cgctcggtca gggcgtgtgg gcggagatta ttcgcaaccg
     4081 gatggcgaaa gaggggttaa tcagcctgat cgataacgcc gaaggcgata acggctggtg
     4141 tctggcgctg gttgagccgg atggcgaacg cacttttatg tcattcagcg gtgtggaaaa
     4201 tcagtggaat cgccagtggc tggcgcgatt aaccgttgcg cctggcagcc tgctctattt
     4261 ttccggttat caactggcct cgccctgcgg cgaattgtta gtggaatggc tggaaaagct
     4321 gcaagacgtg acgccgttta tcgattttgg cccacgtatc ggcgatatcc cggacgcatt
     4381 actggcgcgg atcatggcct gtcgaccttt agtatcactc aatcgtcaag aggctgagat
     4441 tgccgccgaa cgttttgctt tatccgcaga gataacaaca cttggcaagc aatggcagga
     4501 gaaatttgcc gcgccgttga tcgttcgtct cgataaagag ggtgcatggt atttcagcaa
     4561 cgacgcttct ggctgcattc cggcatttcc aacgcaagtt gtagacacca ttggggcggg
     4621 cgacagtcat gcaggtggcg tacttgccgg gctggcctct ggtctgccac tggcggatgc
     4681 cgtattactg ggcaatgcag tggcgtcgtg ggttgtcggg catcggggcg gtgattgtgc
     4741 gccaacgcgc gaggaactac tcctcgcaca caaaaacgta tagatcgctg cgacagtggc
     4801 taatgctgta ctcaataggc cgttgctgtt ggtcaagcgc cacttgcttg atcaccagca
     4861 ccggtatttt gctatctaac tgaatatgtg actgaaactc ggcatccggc atccgggcgc
     4921 taacgcggga acgcgtacgt tgtgggtaaa tgtgctggct gcggaaatag tcataaagtg
     4981 aaatcccgat ggcatcaaca tcatgaatta aatgcgccgg aacccacgat tcctcaatcg
     5041 ataccgcttc ttcatcgaca tagcgaatac gcttaagcaa aaagacatca cttcctgctt
     5101 cgaccgccag ctgctgcgcg acttcttcgg gacatttcac gacacgcttg ttaacccata
     5161 gcgtatcggg ctttttcccg cgcaatacca cctgctgaga aaaacctcgc gcttctttca
     5221 acgagtattc gaagatgttg ttgatctgcg tgccgtaacc gcgcgaacgc gtcaccacac
     5281 cctcttcttc cagcgcctgc atcgccttgc gcaccgtaat gcgcgaaacg ccggttaact
     5341 gacttaaatc acgctcaccg ggcaaaatat tgccatgctc cagcaccccg ctgcgcacgg
     5401 cattttttac cgtttcggca aacttaatat aaagcggcgt gttatccgcc gctaaaatac
     5461 gctcgttcag ttgggcaatc agctgggtat gcgcttgttc catttgtctt tttcctgccg
     5521 gggagtctgg tgggagtata tcactgcttc ttatttaagg cgtttcttta atcccatcaa
     5581 cgaatgcctg caactcctcc accgtgccat gaaatacatt aaaatccacc ggaccattga
     5641 tgccatctac ctgtccacgg tcggaatgct gccagaagcg ccaggccatg ccgtcattgt
     5701 ccggacgacg ttgataatag tgagccaccc accacggata ttcattgaaa tagcccgcca
     5761 gattggtgtg ataaaaaacg gctcctgagt aaataatcgg cttttttccc gtactttttt
     5821 cgaccatttt tagccactga cttactcgtt tgcgtaattc ttttgccgat aattttcccc
     5881 gttcttctac gtccagtacc gcagggaaat cgccttgtga gaaatccacc gtttgcagaa
     5941 ataatcgtgc ctgaaccggc gctgctaccg acggggagaa ataatgatac gccccgcgca
     6001 gcagaccatt ttcgcggctt agttgccagt tacgcgaaaa atacgggtcc accagctttt
     6061 cgccttccgt cgccttaata aaagcaaact gtaagcggat accgttgtcg cgcatttttg
     6121 ccacccgctg ccagtcgatc cgctcctgcc agcgagaaac atctattccg tgaatggtgt
     6181 aactggcagg aatgcgaata gcgaaagatt taaccgggcg ataaccgtag aaatggatgt
     6241 agtctgtaat ctgaactgct gtcgaataga aaaaattcac cgtctgacgc ggataaattg
     6301 caacgatgga aattagccca agagcgcata atagagaggt gagttttttt cgactggtaa
     6361 ttcttagctg cattggtttc atccctgaat gtcagtgcca aaggctgaca ataaccaaag
     6421 cagctatagt acggtgctca ccaccaggcg tggaagtggt gaaccggacc aataccgtga
     6481 ccaacttcca gcgtgtcggc ctgggctaat gctgatgaaa gccagctttt tgcctcctgt
     6541 acggtgtcag cccagtttgt atggcgcggg cgtagtgcag ccaacgccgc agagagcgta
     6601 caaccagtgc cgtgggtgtt tttggtcata atgcgcggtg cggtaaaccg ttgttcaccc
     6661 tcgcgggtaa acagccagtc cgggctttgc tcatcatcca gatgaccacc tttcattagc
     6721 actgcgccac agcccatcgc caacagcgat cgcccttgtt ccagcatttc ctgttcggtg
     6781 cgcgcgtgtg gcgcgtcgag caaggcggca gcttcgggca agtttggcgt tattaatgaa
     6841 acctgtggca ataatcgact gcgcagcgta gcaaccgccg aaggtgaaag cagcgggtcg
     6901 ccgctttttg ccagcataac ggtgtcgagt accacgtttt ggatctgata acgttgcaac
     6961 cgttctgcca ccgcttcaac aatatcggtt tccgccaaca taccgatttt agtggtgtcg
     7021 attcgcacat cgctgaacac cgaatcgagc tgggcggcga caaaatcagg ctcaatgcga
     7081 tacaccgact gtacgccacg ggtattttgc gccaccagtg cagtaataac tgagcaacca
     7141 taagcgccaa gtgccgagaa ggttttaaga tcggcctgaa tccccgcacc accactggga
     7201 tcagtaccgg caatcgtcag agcgttaatt cgtttcatgc ctgcacctcc tgcgtcaatt
     7261 gccagagcgc atcaaggaaa tgtggaacaa aactgcctgg cccctcgctt ctggcgactg
     7321 cgcgctctcc ggcttgtttc atccagtgac aggcagatgc gacattttcc agcgtatcgc
     7381 ctggtaacgc acagcaggca gcgacaaccg ccgataatgc acagccagtt cctaccactt
     7441 tggtcattaa cggatcgcca ccgtgaatgc caacggcgcg atggccatcg gtaacataat
     7501 ccacctcgcc agtgaccacg acgattgcgc cagtttcccg tgccagtgtt tgtgcagcgg
     7561 gtatcgcgtt agctgcggcg tcagtggtat ccactccccg tccaccatta gcaatgccag
     7621 ctaatgccat gatttccgaa gcattaccac gtatcgctgc cggtttaaaa gataaaagtt
     7681 catgacaaaa atggcggcga taatcgagcg caccaaccgc tactggatca agcgtccagg
     7741 gtgtttgaga gctttttgct tgctcaacgg cagcacgcat cgcctgtgcg cgtggctgcg
     7801 tcagtgtgcc aacgttaatc aacaaggcac tggcgatagc cgcaaactga ctggcctctt
     7861 cggtttcgat aaccatcgct ggcgatgcac cgagcgccag caaggtattg gcggtaaagg
     7921 tttgcaccac atcgttggtc atgcagtgca caagagggga atgttggtga aaaaggtgta
     7981 acgcgtgcgc agattgcgct gaactcagca ggtcgacttg catagtttgc tcctgccata
     8041 acgtgaagaa gcaatgacct ggtggtccgt gacttcccta cgctggcatt atccagatca
     8101 ggtgatacgg gtatttctca gccttcacgc agaagggcac cccgagtcgt ttggttgcga
     8161 tgataaatcg cagaggagga tggtaatgtc cagcgcacgc gttgtaaacg aggaaaaacg
     8221 gcagttttac aatcgcgtta ataaattatt tgatatatga atccagcacc ttcagaacga
     8281 catccagatc ttcttcacgt tttagctcat ccccctggtg aacgatgtgt tccgtcagat
     8341 gacctttaat cacttcccgc atcagaccgt ttaccgcgcc acggatagca gcaatctgtt
     8401 gtaaaactgc agcgcattcg tgcggctcgt cgagcatttt cttgagcgcc acgacctgac
     8461 cctgaatctt actggcacgc gctttcagtt tctgtttatc acggattgta tgagacatgg
     8521 caacaccttg ttaacaagaa catgaaaaat catagcacta ttaatctact ggggggtagt
     8581 atcaggtact gggggggagt agaatcagat tgccgaatta atactaagaa ttattatcat
     8641 gaccgaattt acaactcttc ttcagcaagg aaacgcctgg ttcttcatcc ccagcgccat
     8701 cttacttggt gcgcttcatg gcctggaacc agggcactca aaaacgatga tggcggcgtt
     8761 tatcatcgcc atcaaaggca ccattaaaca ggcggtgatg ctcggactgg cagcaactat
     8821 ttcgcatacc gcagtggtct ggttgattgc ctttggcggg atggtggtca gcaagcgctt
     8881 tactgctcaa tcagcagaac cgtggctcca gttgatttcc gcagtgatca ttattagcac
     8941 cgcgttctgg atgttctggc gtacctggca cggcgagcgc aactggctgg agaatatgca
     9001 cgagcatgat catgagcatc atcatcacga tcacgaagat caccacgacc atggacatca
     9061 tcaccatcac gaacatggcg agtatcagga tgctcatgca cgagcccatg ctaatgacat
     9121 taaacgacgc tttgatggta gagaggtcac caactggcaa attttgctat ttggcttaac
     9181 cggtggcctt atcccctgcc cggcagcaat taccgtgctg ttgatttgca ttcagttgaa
     9241 agccctgaca ctgggcgcca cactggtcgt cagtttcagc attggcctgg cgttaacgct
     9301 tatcaccgta ggcgttggcg cagcaatcag cgttcagcag gtcgcaaaac gctggagcgg
     9361 atttaacact ctcgctaaac gcgcccccta tttttccagt ctgttgattg gcttagtcgg
     9421 tgtgtatatg ggcgtacatg gcttcatggg cataatgcga taactcaatc ctttaggctt
     9481 gcatgttatc ttcagcccag gatgattctt aaatcagcta tttccgctga ctctctgctc
     9541 gctaaggatg cttttagggc atcctttcat ttacactttt tacgaaatca tgggatcact
     9601 aacaaaatat cgcttgtcag ttatattgta tggcaggaaa gatatgcgac tgatattaca
     9661 gatccccaaa gtggagagtt tatgaccatt aaaaataaga tgttgctggg tgcgcttttg
     9721 ctggttacca gtgccgcctg ggccgcacca gccaccgcgg gttcgaccaa tacctcggga
     9781 atttctaagt atgagttaag cagtttcatt gctgacttta agcatttcaa accaggcgat
     9841 accgtcccag agatgtaccg caccgatgag tacaacatta agcagtggca gttacgtaac
     9901 ctgcccgcgc ctgatgccgg gacgcactgg acctatatgg gtggcgcgta cgtgttgatc
     9961 agcgacaccg acggtaaaat cattaaagcc tacgacggtg agatttttta tcatcgctaa
    10021 aaaaagcccc ctcatcatga gggggaaatg cagacacctt gttatttttt attattagcc
    10081 acttgctcgt cttgcttgtt attagtcgta tttcacgttg attaatgcgg ttgcctccag
    10141 tgcgccagat ttaactttgt ttgtatcgta gacgtagtaa ctggcagtta tcggaattga
    10201 aaccgttgta tctgtaagtt ttgactttgc cgcaatttct tttaaatttg tgtatccttt
    10261 actgctgtta gtgatattta aaagtgtgac tgacgaacca ttactttcaa gaataaatcc
    10321 tacgccatta tcttccgcac ctttcagtat tgtgttgctg ttggttaaca agtcagaact
    10381 tgataaatac acctgcattt tccgtgatgt tttattttgt tgacctaata aatttttaca
    10441 acgcagaata atatcccctt gtttactatt tattgttgct ttgttacctg tggctggtaa
    10501 ttgtgaaacg ggaatactgt ctaaagtaat taataaatcg tccggtttgc aggttgtggt
    10561 tatcgggttg tatgacaatg tcagattaaa ggaatagaca cctgcatttt gtgccaatgc
    10621 atttctgtag ctatttacac atgcatccca tgacttgcca cctaataaac acagtagaaa
    10681 ttgtactgcg gcactcccgc gtgtcgatga tgaagcactg gataatgtcg caactccatt
    10741 aatcatcact gaaccgccgc cccccgcaga aacattcact tttgcattac tggccgcagg
    10801 tgtcaccgtt accgaataac cactggtgaa actggtctga ttatttgata acgtgatgt
//
LOCUS       DAPGWD010000142        10809 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPGWD010000142 DAPGWD010000000
VERSION     DAPGWD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.143"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(238..1920)
                     /gene="glcA"
                     /locus_tag="RHX88_003541"
     CDS             complement(238..1920)
                     /gene="glcA"
                     /locus_tag="RHX88_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDV2232189.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2276..4447)
                     /gene="glcB"
                     /locus_tag="RHX88_003542"
     CDS             complement(2276..4447)
                     /gene="glcB"
                     /locus_tag="RHX88_003542"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDV2232190.1"
                     /translation="MSQTITQGRLRIDANFKHFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4469..4873)
                     /locus_tag="RHX88_003543"
     CDS             complement(4469..4873)
                     /locus_tag="RHX88_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDV2232191.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(4878..6101)
                     /gene="glcF"
                     /locus_tag="RHX88_003544"
     CDS             complement(4878..6101)
                     /gene="glcF"
                     /locus_tag="RHX88_003544"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDV2232192.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQLVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6112..7164)
                     /gene="glcE"
                     /locus_tag="RHX88_003545"
     CDS             complement(6112..7164)
                     /gene="glcE"
                     /locus_tag="RHX88_003545"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDV2232193.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEVNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7164..8663)
                     /gene="glcD"
                     /locus_tag="RHX88_003546"
     CDS             complement(7164..8663)
                     /gene="glcD"
                     /locus_tag="RHX88_003546"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDV2232194.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            8914..9678
                     /gene="glcC"
                     /locus_tag="RHX88_003547"
     CDS             8914..9678
                     /gene="glcC"
                     /locus_tag="RHX88_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDV2232195.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(9685..10809)
                     /locus_tag="RHX88_003548"
     CDS             complement(9685..10809)
                     /locus_tag="RHX88_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2232196.1"
                     /translation="MIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIERNEHLSA
                     KNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDDPQVFAA
                     LFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQL
                     GYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNS
                     GWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADL
                     NGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFAR
                     RKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
BASE COUNT         2537 a         3025 c         2728 g         2519 t
ORIGIN      
        1 gattgcaaaa gcaacagata gaacggtgtt tgctcatcaa gcgaacataa ttaatacaga
       61 cttgttttgc gtgataaaaa aaatagatgt tctcacgctc ttaattattt agcaggttat
      121 ctgaacacaa aacattattg ccgcatggat aataaagcga gaaataattt tcaattaata
      181 agacagaata atgtaaatga agccggatga tattaacgat catccggcat tattgattta
      241 cgagaccaac attccagtaa acacatacgc ctgcaacaac gtgataacgc cgataacgct
      301 ggcaaaaatc agactgtgct tcacggtgta gcggaatagt tcagattctc ggcccaccat
      361 gcccgtcgcg gcgcaggcca cggcgataga ttgcggggag atcatcttgc cagttacgcc
      421 gccgctggta tttgctgcca ccagcagagt gtcagagacg ttgatttgct gcgccgtggt
      481 cgattgcagt gaaccaaaca gggcgtttga agaagtgtcc gagccggtaa ggaatacccc
      541 cagccagccg aggaacggtg agaaaaacgg gaacatcacg cctgtacctg ccagtaccag
      601 cgccagcgtg gtggacatgc cggaatagtt ggtgacgaag gcgaacgcca gcaccatgcc
      661 aatcgacaat atcggccact tcagactaat tagcgtttcg gcaaagacgc caatcccttt
      721 cttgatgccc acgccgagga tgaagataga gataatcgcc gcaataaaaa tagcggtgcc
      781 gccagccgag agagggtcga atttaaacac cgcatccatt ggcgttggtt gggcgacaat
      841 gggtgccgct ttcaacactt gttgatgcaa atgagggatc tggaaattaa tcaccagtga
      901 ataaaacgcg ccgcccggag caaataacgc tttaaacggc ttcatggtcc agatggtgac
      961 cagcaccgtt aagattaaaa acggtgacca cgcacgaatg atttgcccca gactatattc
     1021 tgaaggcacg ggaccgccag aagatggctt atttaccacc atcgcacctg cggattgccc
     1081 catgctgatt gccgtttcgg tatttttcgg ccgccagact ttaaggaata aagcgagtga
     1141 gacgatactc accagcgccg aagtaatatc cggcagttcc ggaccaatat agttagaggt
     1201 aaagaactga gtgacagcaa agcttccccc agcaaccagc gccgctggcc acgtctcttt
     1261 cacccctttc cagccgtcca tcattgctac cagccagaac ggcacaagaa ccgacaggaa
     1321 cggtaactga cgtcccgcca ttgcgccaat gtggaacgga tcgattcccg ttacctgacc
     1381 ggcgaccaga atcggcacgc ccaacgcacc aaacgccact ggcgcagtgt tggcaatcag
     1441 acacagcccc gccgcgtata acggtttgaa gcccaggccc accagcagcg caccggtaat
     1501 cgccaccggc gcaccaaagc cagccgctcc ttccagtaac gcaccaaagg agaaaccaat
     1561 cagtaacacc tgcaaacgct gatcgtcggt gatggagata accgagctgc ggataatatc
     1621 gaactgaccg ctggcaacgg ttaatttata gaggaacacc gccgcgacaa taatccacgc
     1681 aataggccat aatccataaa taaagccata gcccgcagca gcaaaagcca tatcaatcgg
     1741 cattttaaag gcgaatattg caatcaggat agataatata agggttattg ctccagcgac
     1801 atgtcctttc agacgtaata ccgcgagtgc aacgaagaag aatattatcg ggatcagggc
     1861 gaccagagcg gatagcccca gtcctcccat cggcatatac atttgggtcc aggtaaccat
     1921 attgttcagt ctcttattat ctttatatgc ttgatatact taaggttgta ataagcaaaa
     1981 gaggactgaa ctgtaaaata taggcgttat actttacagc aacagtacgc cgctaacgca
     2041 attgctacct ctggcataac aagtatatcg ggtaagggtt tctgttctgc acacgcaggc
     2101 gcagagtatc gttaagatgt ccatattgtt gttataggcc cgctagtaat gcgctacggg
     2161 tatttaatat tgttaaaccc tgataatcgc tccggttatt tccgggataa atgtactacc
     2221 gcagttacta tcatagcccc gacaataaaa cttgtcgggg ctttttttga cgctattaat
     2281 gactttcttt ttcgcgtaaa cgccaggcgt gtaataacgg ttcggtatag ccgtttggct
     2341 gtttcacgcc gaggaagatt aaatcgctgg cagctttaaa agcacacgag ttagcgaaat
     2401 tccccgccat cggacgataa gccggatcgt cagcgttttg ctgatcaacc actttcgcca
     2461 tattctccag cgacgcctgc acctgttctt tggtcagaat accgtgacgt aaccagttgg
     2521 cgatatgctg gctggagata cgcagcgttg cgcggtcttc catcagcgcc acattgtgaa
     2581 tatccggaac ttttgaacaa ccaatcccct gctccaccca gcgcaccacg taccccagaa
     2641 tcccctgcac gttgttatcc agctcttgtt ggatctcttc caccgaccag ttagcgtttt
     2701 cagccaccgg aatagtcagc agatcgtcca gcagcggttc aaattcagca ttgaactcgg
     2761 tctgggcaat gttggcttgc acgctctgta cgttggtttg gtggtagtgc agcgcatgga
     2821 gcgtagcagc ggttggtgac ggaacccagg ctgtgtttgc cccggcacgc agttggtcgc
     2881 ccttctggct gtacatgtct gccatcaggt ccggcattgc ccacatgcct ttaccaattt
     2941 gcgctttacc gcgcagccca cagaacagac cggaaagcac gttattacgc tcgtaggctt
     3001 tgatccaagg cgtcgatttc atctgatttt tacgcagcat cgggccagct tccatcaccg
     3061 aatgcatttc atcgccggta cggtcgagga aaccggtatt gatgaacgcc acgcggttgc
     3121 gcgcctgagc gatacagcta cgcaagttca gcgaggtccg acgttcttca tccataatgc
     3181 ccattttcag ggtattcggt gccataccga gcattgtctc aatgcgggta aacagtttgt
     3241 tggcgaacgc cacttcctgc ggaccgtgca ttttcggttt cacaatatag acgctgccag
     3301 tgcgcgagtt tttctgcact tttaaatcat agagggcgat cgcgccagtc atgacgccat
     3361 caagaatgcc ttccgggatt tcattgcctt cgctgtccca aatcacagga atggtcatca
     3421 aatgacccac gttgcggata aacagcagcg agcgtccgtg cagagaaatt tcagagccat
     3481 cggcggcggt gtaatgacga tcgtcattca gtttacgcac gatttgtcga ccgtttttct
     3541 ccattttctc ttgcagagtc ccctgcatca ggcccagcag attgcggtac agcaggattt
     3601 tatcttccgc atcaaccgcc gcgaccgaat cttcgcagtc gagaatggta ctgatggccg
     3661 cttcgacgat aacatcgttg atgtgcgccg gatcgtcttt gccaatccgc ccgttggcat
     3721 caatttgcag ttcaatatgc aggccgttat ttttcagcaa aatgcaggtc ggcgcagcgg
     3781 catcgccacg gtaaccgaca aactgcgccg gggtacgtaa cgtggtttct ttaccatttt
     3841 tcaactggat gcgtaattgt ttatcgacca ccttaaacgc caccacatcc tgatagctgc
     3901 cgttttccag cggtagagat tcatcgagga aacgccgaac ccaggcgata acctgctcac
     3961 cgcgttgcgg atcgtagccg ctgaccatcg ccccttcctg cgggatgatg tcgctgccgt
     4021 ataacgcatc gtacagtgag ccccagcgag cgttcgccgc gttcagcgcg tagcgggcgt
     4081 tcattgccgg aaccaccagc tgtggccccg cctgactggt gatttcgctg tcaatgcccg
     4141 tggtttccac cgtcacgcgc tccggttgcg gcaccaggta gcccagttca cgcaggaaag
     4201 atttataggc cgctttatct tttaccggcc ccggattgct gcgatgccac tcatcaagcg
     4261 ccgcctgaat gcgatcgcgt tctgccagca actgacgatt ttctggtgcc agatcgtgaa
     4321 cgatctcatc aaaattgcgc cagaacgccg cagcgtccag ccctgttccc ggtaaaactt
     4381 cttcatccac aaaatgttta aaattggcgt caatgcgtaa acggccctgg gttatggttt
     4441 gactcattgt ttctctcctc gttttcgctt atttcgccaa caccgctgct gccgctttcg
     4501 cgacctgcgc atcctgtgct ccggttaaac cagaaacgcc cacggcacca ataatttgcc
     4561 catcaacaac aaccggtacg ccgccttcca gcgacgttaa taacggcgca gtcacgaacg
     4621 cggtacgtcc gttgttcacc atctcttcat agcccttagt ttcacgacgc cccagcgcgg
     4681 cggtacgcgc tttctcctgg gagatgtaag ccgcaatcgg cgcgcaatcg tccatgcgac
     4741 ttaacgccag cagatgaccg ccgtcatcgg caacagcaat ggaaacagac cagttgtttt
     4801 tctgcgcctc ttcctgacct gcggcaataa ttgcactcgc catttgctgg ctaagaatga
     4861 ctttagtttt cattttgtta ttccttttca agggcttgtt ctacaatttc aatccagtga
     4921 cgcacagagg tacgaccggc gctcgccaga tgcgtctggc aaccaatgtt ggcggtgacg
     4981 atcatttccg gtttgccgct ttccagcgca ttcattttgt tatcccgcag ctggcgtgcc
     5041 agatcgggat gcgttaacgc atatgttccc gctgaaccgc agcacagatg gctgtcggga
     5101 acgtccgtta aggtaaatcc aagacgaagc aacacttttt ccacttcgcc gttcagcttt
     5161 tgcgcatgtt gcagggtaca cgggcagtgg aaggccagtt ttttatcgcc gcgaattgcc
     5221 agtttttcca gcggttcctc gcgcagaagt tcgactaaat cgaccgccag ttcactgact
     5281 tgacgcgctt tatcggcata taacgcatcg tttttcagca tctgcccata ctctttgacg
     5341 aacgcgccgc aaccgctggc ggtttgcaaa attgcctcgg cacctgcttc aatcgcgggc
     5401 caccaggcat caatattatt tcgcgcccgt gccagccctt tctcctgcgc attaagatga
     5461 tagtccaccg cgccacaaca gcctgcttcg ttagctgaca tgacgctgat ccccagacga
     5521 tccagcactc gcgcagttgc cgcgttggtg ttgggcgaaa gcgtaggctg ggcgcagcct
     5581 tccagcatta aaacccgacg cttatggcgc agcggcggac gcggtttagc tttcacagtt
     5641 tcagcaggca gttttgctct gacctgttcc ggtaaaaacg gtcgcagcac cagccctacc
     5701 tgcgtcagcg cacggaagac cgccggacgc ggcactaact ggcgcaatcc ttcgcgcagt
     5761 attcgctccg gcagtgggcg tttcactttc tgctcgacaa tatcacgccc gatatccagc
     5821 aaattgtgat agcgcacacc agaaggacag gtggtttcac aattgcggca agtgaggcag
     5881 cgatcgagat gctcctgtgt tttaagcgtg acttcgttgc cttccagcac ctgtttaatc
     5941 agatagatgc gcccgcgcgg cccgtccagt tcatcgccca gaagctgata ggttgggcag
     6001 gttgcggtac aaaatccgca gtgaacacag gcgcgcagga tgctgtcggc ttccagcgcg
     6061 cgcgcgttct gccgcatctc ttcagttaat tgggtttgca tagcctgctc ctcaaagttc
     6121 cgcgtacatg cgaccggggt taaacacgcc gcaagggtcg agctgctgtt taagctgctg
     6181 gtgatagcgg aataaaggag ccgatagcgg ggcaaagcca ccatctccgg cactaaagcg
     6241 ggtcgcatga ccgccagcgt tgcgggcgat gcgatggatt tgattgacct cggctgtcga
     6301 tttcagccag cgtaacgcac cgccccagtc gatcagttgc tcgccgggta aatccatcat
     6361 cggcgcatcg ctgggtaatg aaatgcgcca taaggtacct ggtaacgaga agaacggcag
     6421 ttgttgttca cgcaattgct gccagaactg acccgcaacc tcttcgcccc ccagcagttc
     6481 acgcgccgct tttaccgatc cttcgccgcc ctcaaggcgg atccacaacg cattgtcgaa
     6541 gtaacataag ccactaatag gtagcggctg gagttgccac tgggcgattt cgttcatggc
     6601 ttcttgcagg ctgatttccc gacgcaggct cagggaggcg cgcggtcgcg gtaacacttt
     6661 catcgagatt tcagtgagca cgccaagaca accgtagctt ccggccatta accgtgagag
     6721 atcgtatccg gcaacgtttt tcatcacttc gccaccaaaa cgcagatgtt ttccagcgcc
     6781 ggtaatgatg cgcgtgccga ggacaaaatc gcggaccgaa ccgctccacg ggcgacgcgg
     6841 ccccgccagc ccgcaggcga ccatcccgcc ccaggtggct tcttcaccat aatgcggcgg
     6901 ctcgcaggga agcatttgcc cggcgctctc cagcgccgct tcaatcgcca ccagcggcgt
     6961 tccggcacgc gcggttatca ccagctcggt cgggtcgtaa ttaacaatgc cgcgatgaca
     7021 acgcacatcc agcgtttgcc cggtgacagg gcgacctaaa aaggctttgc tattgctgcc
     7081 ctgaatcacc agcggcgttt tatcgctaat tgcctgattc acctgctcca gcagcgcctg
     7141 gctgtaatca cactcgcgta gcatcagaaa cgctccagtt cagggaaagg taaatgaccg
     7201 tgatgcacat gcatggcacc aaattcagca cagcggtgta gcgtgggaat gtttttccca
     7261 gggttcagca aaccatcggg gtcaaacgcc gccttgaccg catggaaggt cgtgatttca
     7321 tcgctgttga actgggcgca catttgattg attttttctc gcccgatgcc atgttcccca
     7381 ctgatgctgc cgccaacttc aacgcagagt tcgaggatct tcccacccag ctcttccgcg
     7441 cgggcaaatt caccgggttc gttggcatcg aaaaggatta acgggtgcat gttgccatct
     7501 ccggcatgaa agacgttggc aacacgtaaa tcatattgct gcgataaacg ggcaatgcct
     7561 tccagtacgc caggcagggc gcgacgcggg atggtgccat ccatgcagta gtaatccggg
     7621 gagatacgtc ctaccgccgg gaacgcattt ttgcgaccgg cccagaaacg tacgcgctct
     7681 gcttcgtcct gtgccagacg gacgtcagtc gcgcccgctt tcaacaagat gtcgttaacc
     7741 cgctcgcagt cttcctgtac gtcagactcc acgccgtcca gctcgcataa caaaattgct
     7801 tcggcgtcga cgggataacc ggcatgaata aaatcttccg ccgcgcggat cgacaggtta
     7861 tccatcatct ccagcccgcc ggggataatg ccattggcga tgatgtcacc aactgcaagt
     7921 ccggcttttt ctaccgagtc aaagctggct aacagaaccc gcgccacggg cggcttcggc
     7981 agcagtttta ccgtcacttc ggtggtcacg ccgagcatac cttccgatcc ggtgaacagc
     8041 gccagcaggt caaaaccagg tgaatccagc gcgtccgatc ccagcgtcag cgcctcgccg
     8101 tccagcgttt gcacttcaat tttcagcagg ttatgtacgg tcagaccata tttcaggcag
     8161 tggacgccgc cggcattttc tgccacgttg ccgccaatgg aacaggcgat ttgtgaggaa
     8221 gggtccggtg cgtagtagag attatgcggt gcaacggcct gggagatcgc caggttacgc
     8281 acgcctggct gcacgcgcgc gcggcgacca acggggttaa tgtcgaggat ctctttaaag
     8341 cgcgccatca ccaacaacac acctttttcc agcggcagcg cgccaccaga aagcccggtg
     8401 cctgcaccac gggtcaccac cggtacacgc aggcgatggc agacagcaag aatcgctgtc
     8461 acctgttcca tttgcttagg cagaacaacc agtaatggac gcgtgcgata cgcgctcaac
     8521 ccgtcacact cgtaaggaat gatctcctca tcggtatgca ggatctcaag tccagggaca
     8581 tgctcacgca gtgccatcag taccgatgtg cggtcgacat cgggtaaagc gccatcaaga
     8641 cgctcttcgt acaagatgct catgagtagg cttcgctttg ttgtgttgtg tggcagctga
     8701 tttttgcgcg ctgcttctgt gaacagttat taagcgggct tttcgttttc gtctatctct
     8761 ttagctaccg gtcagaccat tttttttcca gctctgtgac cttgccttgg ttaactcaat
     8821 gttaaattga tgtaacataa tcacttacgt gatgtgcgtg ttttgcgagt taagaacaga
     8881 aaaattggtc ctacctgtgc acgaggtccg ggaatgaaag atgaacgtcg ccctatttgc
     8941 gaagtggttg cagagagtat cgaacggtta attatcgacg gcgtactgaa ggtcggtcag
     9001 ccacttccct cggaacgtcg actgtgtgaa aagctcggct tctcacgctc cgcactgcgt
     9061 gaagggctga ccgtgctgcg cgggcgcggg attattgaga cggcgcaggg tcgcgattct
     9121 cgtgtcgcac ggcttaatcg ggtgcaggac accagcccgc tgatccatct gttcagtacg
     9181 cagccgcgaa cgctgtacga tctgctcgac gttcgcgcat tactggaggg cgaatcggca
     9241 aggctggcgg caacgctggg aacgcaggct gattttgttg tgataacccg ctgttatgaa
     9301 aaaatgctcg ccgccagtga gaacaacaaa gagatttcgc tgatcgaaca tgcgcagttg
     9361 gatcacgctt tccatctcgc catttgtcag gcttctcaca atcaggtgct ggtgtttacg
     9421 ctgcaatcat tgaccgatct gatgtttaat tcagtgtttg ccagcgtaaa taatctctac
     9481 catcgaccac agcaaaaaaa gcagatcgat cgccagcatg cgaggatcta caacgcggtg
     9541 ttgcagcggc tgccgcacgt cgcccagcgc gcagcacgcg atcatgtgcg gaccgtgaaa
     9601 aagaatctcc acgatatcga gctggaaggc caccatttga ttcgctcggc ggtgccgctg
     9661 gagatgaacc tgagttagct ggtattaaat ctgcttttca tacaatcggt aacgcttgta
     9721 cggctccgcc ccaatgcgtt ccagcatgtt attcatgcct gtattggttt cgaggatcca
     9781 tgacatctcc agcgcatcga tcttccggcg ggcaaacgga tcgcgtaagg cttcaatcaa
     9841 taacagcgca atcaccgggc cgatgcggct gaactgatac tcgtcgcgca cgcccatcag
     9901 cggtactcgc gcagttcgca caccgctgac tttcaaacgc cacagcaatt ttgcccagcc
     9961 gaagggaaag agcgatccgt tcagatcggc aatcgcctcg ttgatgttcg gcaagccgac
    10021 aataaacgcg cagggtgcag aatcaatctc agcgatatag atcatatcgt ccggcaccag
    10081 atatttaagt tgatcgccca tggtcgcgaa ttcatgttcg gtaaacggca caaatcccca
    10141 gttgtgctgc cagccagagt tgaaaatctc acgcaggatc tgcatttctt cggcaaaccg
    10201 ctgacgattg atgcagcgaa tggtcacctt tttgcgcacc tgatccatca gtttttttag
    10261 cgccggagag aaagtgagat cggttcgctg catccaccac gccagtaaat caatgccttt
    10321 gtgataaccc agttgttcaa tatgcgcggc ataccacggt ttgccgtgtg gcatcatcgc
    10381 acagggtggt gtgtcaaaac cttcaatcag taatccgctt tcctgattga tattcaggct
    10441 gaaaggaccg ctgatcttac ttgcaccttg tgacttcaac cacgcttccg ctgcgccaaa
    10501 caacgcggca aaaacctgcg gatcatcaat ggcgtcaatc atgccgaaat gaccggtatc
    10561 ttcaccgtaa cgctcacggt gcaaggtatc tatttgcgcg gtaatacgcc caactatctg
    10621 ccccgctttt tttgccaccc acgcctgcca gatgatatgg tccgtccccg gatttttcgc
    10681 agacaaatgc tcgttgcgtt ctatgaataa agggggtatc cagtttggat cgtcgggata
    10741 aagtgatgac ggaaaagcga taaatgcctt aaggtcattt ttattaatta ctttttcaat
    10801 tttaatcat
//
LOCUS       DAPGWD010000143        10766 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPGWD010000143 DAPGWD010000000
VERSION     DAPGWD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.144"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            148..861
                     /locus_tag="RHX88_003549"
     CDS             148..861
                     /locus_tag="RHX88_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV2232197.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILEVDGR
                     QPADEILQASLRAIHAALS"
     gene            893..1651
                     /locus_tag="RHX88_003550"
     CDS             893..1651
                     /locus_tag="RHX88_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232198.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRSYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1697..3046
                     /locus_tag="RHX88_003551"
     CDS             1697..3046
                     /locus_tag="RHX88_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDV2232199.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3046..3870
                     /locus_tag="RHX88_003552"
     CDS             3046..3870
                     /locus_tag="RHX88_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232200.1"
                     /translation="MMRKYFPLEARERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIVKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            3882..4442
                     /locus_tag="RHX88_003553"
     CDS             3882..4442
                     /locus_tag="RHX88_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV2232201.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4473..5528
                     /gene="lptF"
                     /locus_tag="RHX88_003554"
     CDS             4473..5528
                     /gene="lptF"
                     /locus_tag="RHX88_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV2232202.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSSANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLKPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRIKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLL"
     gene            5540..6619
                     /gene="lptG"
                     /locus_tag="RHX88_003555"
     CDS             5540..6619
                     /gene="lptG"
                     /locus_tag="RHX88_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV2232203.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     EILDNLAWQSTFTSMNLEELSMPGNSFSLRQLNHYINYLQETGQPSSEYRLALWEKLG
                     HPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VAALGLPMAFVLAALILVYLFDRQH"
     gene            complement(6654..7826)
                     /locus_tag="RHX88_003556"
     CDS             complement(6654..7826)
                     /locus_tag="RHX88_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV2232204.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFK
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7826..8074)
                     /locus_tag="RHX88_003557"
     CDS             complement(7826..8074)
                     /locus_tag="RHX88_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV2232205.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8106..9020)
                     /locus_tag="RHX88_003558"
     CDS             complement(8106..9020)
                     /locus_tag="RHX88_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV2232206.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(9017..10747)
                     /locus_tag="RHX88_003559"
     CDS             complement(9017..10747)
                     /locus_tag="RHX88_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDV2232207.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTVQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
BASE COUNT         2602 a         2669 c         2806 g         2689 t
ORIGIN      
        1 atccaaaata tttatttggt taatatgttt ttatgaaagc gtaattcagg tcaatgtcac
       61 aattaaccat tgtcacaata aggttgaacg gatattctga gcacggacca cgtaatattc
      121 aatacattat ttactgccgg gtttttcgtg aatcaacgta atatgtcaat aattaattcc
      181 acgccagtgc gtgttattgc cattgtagga tgtgatggtt caggtaaatc gaccctcacg
      241 gcaagcctgg taaatgaact ggcagcaaga atgccaacag aacgcattta tctcgggcaa
      301 tcgtccgggc gaattggcga atggatttca cagctccctg ttattggcgc accttttggg
      361 cgttatctgc gaagtaaagc ggcacatgtg cacgaaaagc cctcaacacc gcctggcaat
      421 attactgcgc tggttatcta tctgctttcc tgctggcggg cgtacaagtt tcgcaaaatg
      481 ttgtgtaaaa gccagcaagg ctttctgctc atcaccgacc gctacccgca ggttgaagtg
      541 ccggggtttc gctttgatgg cccgcaattg gcaaaaacca cgggcggtaa cggttggata
      601 aaaatgttaa ggcagcgcga gctgaagctg taccaatgga tggcgtctta tttgcccgta
      661 ttgttgattc gtcttggcat tgatgaacaa accgcgtttg cgcgtaaacc tgaccaccaa
      721 ctggcagcgt tacaggaaaa aatcgccgtt acgccgcaac tgacattcaa tggtgcaaag
      781 atccttgaag tggatggtcg gcaaccagcc gatgaaattc tgcaagcgtc actacgcgca
      841 attcacgccg cgctttcctg atcaccaaaa tggatagggg atgatttaac gaatggatgc
      901 actacaaaca caaactgtta ttagtacaac cgcgccgcag cccagctaca ttccggggct
      961 aattgcggtg gtcgggtgtg atggcaccgg taaatccaca ctgaccaccg acctggtgaa
     1021 atcgctgcaa caacactggc aaaccgagcg gcgctatctg gggctgctct ccggcgaaga
     1081 cggcgacaaa atcaaacgat tgccgttggt tggcgtctgg ctggaacggc gactggcggc
     1141 caaatcctcg aaaacccaaa gcatgaaaac caaatctccg gcgctatggg cggcggtgat
     1201 tatgtactgc ttctcgctgc gaagaatggc gaatctacgc aaggttcagc gactggcgca
     1261 aagtggcgtt ctggtggtca gcgatcgctt cccgcaggct gaaatttcgg gcttttatta
     1321 tgatggaccg gggattggcg tcgaacgtgc gaccgggaaa atcagcatgt ttctggcgca
     1381 gcgcgaacgg cgttcatacc aacaaatggc gcaatatcgc ccggaattaa ttattcgcct
     1441 gggcattgat attgagactg ccatctcccg caagcctgac catgactatg ccgagctgca
     1501 ggacaaaatc ggtgtcatgt cgaagattgg ctataacggc acaaaaattc ttgagattga
     1561 ttccagagcg ccctacagcg aagtgctgga acaggcacag aaggcggttt ctctggttgc
     1621 catcgtttct gaccgccgaa gtttaactta aggctggcgg gatacttttt actatcaacg
     1681 ataaggtcag gctgaattgg cgggttttaa catcaaacat tggtttgcag atggcgcgtt
     1741 tcgcaccatt attcgcaata gcgcctggtt aggctccagt aatgtcgtga gcgccttgct
     1801 ggggctgttg gcgctctcgt gtgccggtaa agggatgacg cccgccatgt ttggcgtact
     1861 ggtgattgtg caatcgtacg ccaagtcgat cagcgatttt attaagtttc agacatggca
     1921 actggtggtt cagtacggaa caccagcatt aaccaacaat aatccgcagc aattccgcaa
     1981 tgtcgtctca ttttccttct cgctggatat cgtcagcggc gcggtggcga ttgtcggtgg
     2041 cattgcctta ctgccattcc tttcccattc attaggtctg gatgaccaaa gcttttggct
     2101 ggcggcgctg tattgcacgc tcattccttc aatggcttcc tccacgccga ccggcattct
     2161 gcgtgcggta gatcgcttcg atttaattgc tgtacagcag gcgacgaaac cttttctgcg
     2221 cgcagcgggg agcgtcgtag cctggtattt tgactttggt tttgcgggtt ttgttattgc
     2281 ctggtacgtg tcgaatctgg ttggcggcac catgtactgg tggtttgcag cgcgcgaatt
     2341 acgccgcaga aatatccata atgccttcaa attgaatctg tttgagtctg cccgacacat
     2401 caaaggcgcg tggagttttg tctggtcaac caacattgcc cactccatct ggtcggcgcg
     2461 taactcgtgc agcacagtgc tggtggggat cgtgttagga cccgctgccg ccgggttatt
     2521 taaaatcgcc atgacattct tcgacgccgc cggaacgcca gcgggtttgc tgggtaaaag
     2581 tttttaccca gaggtgatgc gtttagatcc gcgcaccacc agaccgtggt tgttaggtgt
     2641 gaagtccggt ttactggcgg gcggaatcgg tattctggtg gcgctcgcgg tgtttatcgt
     2701 cggcaagccg ctcatttcgc tggtgtttgg cgttaagtat ctcgaagcgt atgacctgat
     2761 tcaggtgatg ctgggcgcaa ttgtgatttc gatgctgggc ttccctcagg agtcattgct
     2821 attgatggcg ggaaaacagc gcgcttttct cgtggcgcaa accatcgcct caatcggcta
     2881 catcgtattg ctgttcatgt tctgtcatct gtttggtgtg ctgggagcag cgtttgctta
     2941 cttcggtggg caatgtctgg acgtggcgct ctcgttaatt ccaaccttaa aggcattttt
     3001 tcagcgccat tccttgcttt ataacgcagc cggagagaaa tcctgatgat gagaaaatat
     3061 ttcccgcttg aggcgagaga gcgtctattt gttgcgattg aagaagatga tgtcgttgat
     3121 gcacaggtta gtttgccgcc gactattgca ctaagttgta ccaccgaaat cattcatgat
     3181 aattacgcgt tatgcctgca attctggctc aatggcgttg atcgtcagga attactgcgt
     3241 ttagttcgca aacaagccaa aggcgatgag ctgactgctg atgagcgcaa acaatttaaa
     3301 tacatgcgag cgcgctacaa gcacctgcgt tttgcgcaac ggctgtattt aaagaagcat
     3361 caggccggat ttttattcgg caaaacgacc gtttttctgg ggcgttttca ggacggcttt
     3421 cgtaacggta agaaaaatat tgtgtcgtat tacggcaacc tgttacggat ttatttaagc
     3481 tctccggtct ggtcgttggt gaactattca ttacgccata gccagttaga aagcgtaagc
     3541 agttttattg cctatcggca aaagcaaatg cataccctga aagagattat tgtcaaaccg
     3601 cgactaaccg gcagggaatt tcatgatgtg cgaaaaatca tcagccaaca ggtttcttat
     3661 tacgacacct taagatcgct tgatcctgaa aataaagagg cgttacagat ttcccgtttc
     3721 ctcgccgcca ttaatggcct gatgggagat aaacacgacg acatggtggc ggatgatatg
     3781 gaaaaccgcc agtcgtatga tgcaccgttg gcgctggata gcgatattcg tcagcgctta
     3841 gagctgctga tttcgcgttt tccgctatag ccggagcaat gatgtcatct cgtttgatta
     3901 ttgcgttgat tatcatgctc ttagccccgg gtgtgcaggc acacaatttc gtcactggca
     3961 aaacggttac ccctgtttat atccaggaag gcggcgagct gcttctgaat agcgacgacg
     4021 aaattcacta ccaaaagtgg aacagcacac aattagcggg aaaagtccgc attattcagt
     4081 atatcgccgg gcgtaagtcg gcgaagaaaa agaactcact gttaattaaa gcggttgaag
     4141 cggcaaactt tccgcaggat cgttttcagc ccaccaccat tgtgaacacc gatgatgcca
     4201 tctttggtac cggctatttt gtcgttggca aaatcgaaaa aaataaacgc cgctatccgt
     4261 gggcgcaatt cgttattgac ggcaatggac agggacgcgt tgcctggcgc ttgccggagc
     4321 agagttcaac cattctggtg ctgaataaag ccgggcaaat tcagtgggca aaggacggca
     4381 gcctgacgcc ggaagaggta gatcatgtca ttgcgttggc gcagaaactg atcaatgaat
     4441 aaaaaactca aaggattgag ggcgttagtg agatgaagct ggttgaacac tacatcatgc
     4501 gtggtacgcg ccgtctggtg ctgattatcg tcggttttct gatctttatt ttcgccagct
     4561 actccgcaca gcgttatctg accgaagcgg caaatgggac attagctctt gatgttgtgc
     4621 tggatatcgt tttctacaaa gtactgattg cactggagat gttgttacct gttggtctgt
     4681 atgtgtcagt tggcgtgacg ctagggcaga tgtataccga ctcggaaatt accgctatct
     4741 ctgcggcggg gggcagtccg ggacgcttgt acaaagccgt tctttatctg gcgataccgc
     4801 taagtatttt tgtcaccctt ctgtcgatgt atggtcgtcc gtgggcttat acgcagattt
     4861 atcaactgga gcagcagtcg cagtcggagc tggatgttcg ccagttgcgg gcaaagaaat
     4921 ttaacactaa cgataacgga cgaatgatcc tttcgcagac ggttgatcag gataataatc
     4981 gcctgactga cgcgctgatt tatacttctt ctgccaatcg aacccgcatt ttccgcgccc
     5041 gttcggttga tgtggttgac ccatcacccg agaaaccgac cgttatgttg cacaacggca
     5101 ctgcctatct tctcgatcat caggggcgtg acgacaacga acagatctac cgtaatctgc
     5161 aattacatct gaaaccgctg gatcaaagcc ctaacgtcaa acgcaaagcg aaatcggtca
     5221 cggagctggc gcgctccgcc tttcctgccg atcatgccga attgcaatgg cgacaaagcc
     5281 gtggcctgac agcattgttg atggcgctgc tggccatttc attaagtcgg ataaaaccgc
     5341 ggcaagggcg attttcaaca ttattgccac tgacgttgct gtttattgcc attttttatg
     5401 gcggcgacgt ctgccgtacg ctggtggcta acggcgcgat tcccctcatt cctggtttgt
     5461 ggttagtacc cggactcatg ctaatgggcc tgctgatgct ggtcgcacgc gacttctctt
     5521 tgctgtagaa attttcccga tgaatgtttt cagtcgctat ttaatccgtc atctctttct
     5581 cggttttgcc gccgccgcag ggttattgct gccgcttttt actaccttca acctgattaa
     5641 cgaactggat gatgtcagcc cgggcggtta tcgctggact caggcggtgc tggtggtgct
     5701 gatgacctta ccgcgtacac tggtcgaact ttcgccgttt atcgcattat tgggagggat
     5761 tgttggcctg gggcagttat cgaaaaacag tgagcttacc gccattcgca gcactgggtt
     5821 ttctatcttc cgtattgcac tggtggcgct ggttgccggg atattgtgga ctgtttcgtt
     5881 aggcgcgata gatgagtggg tagcgtcgcc attgcagcaa caggcgctgc aaatcaaatc
     5941 gaccgccact gcgttggggg aggacgatga tattaccggc aatatgctgt gggccaggcg
     6001 cggtaatgaa tttgtgacgg tgaaatcgct gaatgaacag ggccagcctg tgggcgtgga
     6061 gatatttcat tatcgcgacg atctctcgct cgaatcctac atttatgcac gcagtgccac
     6121 cattgaagac gacaaaacgt ggattctgca tggtgtgaat cataaaaaat ggctgaatgg
     6181 caaagaaacg ctggaaatat tagataatct tgcctggcaa tcgacattca ccagtatgaa
     6241 tcttgaagaa ctgtcgatgc cgggcaatag cttttccctt cgtcagctca accattacat
     6301 caattacttg caggaaaccg gacaaccgag cagtgagtat cgcctggcat tgtgggaaaa
     6361 gctggggcat cccattttaa ccctggcgat gattttgctg gcagtgcctt ttacctttag
     6421 cgccccgcgc tcgccgggga tgggcagccg tcttgctgta ggcgtcatcg ttggcttact
     6481 tacctggata agctaccaaa taatggtcaa tctgggatta ttatttgcgc tgagtgcgcc
     6541 cgttgctgca ctcggtttac ctatggcatt tgtgctggcg gcgttgattc tggtgtatct
     6601 atttgatagg caacattaaa cccgccgtgg cgcaacacca cggcgggctc acattactgc
     6661 ttcagagtag caaatgcctg aatgatggca tcgatttgct cacgactgtg ggcagcgttt
     6721 acgctacagc gcagcagcgt gatgcctgca ggcgcggcag gtggcaggat aagattcaca
     6781 taaacgccca gcgaaataag ctcgcgccag atgcgtaaac cttcctcttt cgaaccgatt
     6841 attaccggca ctaccgggct aatatgtgaa cttaactcat aacccagttt ttgcagaccg
     6901 tcatacagat ggttggcgtt atccatcagc ttttgccgca gctctggttg tgtggcaata
     6961 gttcttaacg acgagcgcac ggtcgcaatg caggacggag aaggagaggc ggtaaaaata
     7021 tacgggcggc tgccgtagcg cagcacttcc atcgcttccg aaccgacagc aaaaccgcca
     7081 atcgaggcca gacttttact gaaagtacca acgataatat ccacatcatc ttcaacgccg
     7141 acagcttcag ccagtccgcg ccccgttgcg cctaacacgc caaacgaatg cgcttcatcg
     7201 acaatcaaat aaccaccgag acgacgtttg atatcaacaa tttccgccag cggcgcaacg
     7261 tcaccgagca tgctgtagat gccctcaacg atgataatcg cctctttcgc ccgttcaccg
     7321 aggcgaacca tgcgccgttc cagatcttta gcatcgttat ggcggaaacg aataatttcc
     7381 gcgccaccga gagaacaggc gtcataaata ctggcgtggc tgtcggcatc cagtaacacc
     7441 acggcgttat ggtcggcaag tgcgctgatg acgcctaaat tagcggtata accggttgaa
     7501 aaaacgatag cggtagggcg cttgaaaaac tcggcaattt ctttctctaa cgccaggtgc
     7561 ggcgcataac tgccgtttgc catacgagaa ccggtggtgc cggtgccttg ggccgctaac
     7621 gctgcctgac cttcgctgat ggcatcatga ttaaaggtta aaccgagata attattggtc
     7681 ccggccagaa tcactttttt attgccgatc cggccttcgg tggcggaata cacttcatca
     7741 atacaggtgc caaacggtgt taacccgttg tcactaaatt gcagacgctc gccagcaagg
     7801 cgcgcatatt tatcgtatag ccccattatt tattctccag ccatggaagt agagtttcca
     7861 ttaattgcgc aggggttttg acatccagca gaatattaat aggaatggaa atatcgaatc
     7921 tgtcttccag catcattaag agatccatca ctttgatcga ctccaggcca agatcgttga
     7981 cgagatcgct gtcgggctta atttccacac cgttttcgac caaatcctgt aaacaggaaa
     8041 ggatgtaatc cattactatt tcacgattta ccatagaaac gttaacacac ctttaaacaa
     8101 gacaatcaaa atagcccgtt gcgtaatgcg tgttccaggc taatcccggg aaaccaatta
     8161 atatcttcag aaatacggtt attacttgcc gaccagtcgg cgtgggttaa ttcacgaatt
     8221 ttcgagcggg tcagcatagg ttctttacct gccaggcgac tcaacgcggt actgatatcc
     8281 gcaaggcagg tgagtaccgg cagaggaata ccaaccattc gcacggaacc gcaacggacg
     8341 ttggcggcaa gttgctgtat gcgttgccag tcatagccgc cagcgacgcc atcgcataat
     8401 tcataggttt gcgtctgtac agtttcggcg cttaaccact gacccactgc ttgcgcaaaa
     8461 tcggtgacgt gcaggaaaga gagctgtgta tctggtgcac caagtcgtgg cagcaggccg
     8521 cgcagcatcc agtcaaacag cggttttaac tctttatcgc cgggaccata cacggctgtc
     8581 gggcgaaaaa cgcccagcgt aatttcgtca gccattgcag tcagccgttg ttcggcgatg
     8641 tgtttggaat ttgcgtacca ggagagctcg ggatggcgcg ccgccagcga agagataaac
     8701 agaaaacgtt ggcaaaagca gctctctttt gctgcttgca tcaggcgcag gctgccgtca
     8761 acgttacagc gggtgaaaat ctcttctttg tgcccgcgca cttgcccggc gcaatggacc
     8821 actgcgctgg ctccggcaac cagctcgcta agtgaatgag tatcttccag cgaaccgcgc
     8881 acccaggtga gattatcatt gacgtgagcg cgggcagtac gcgtcaatgc gcgaacgtga
     8941 aagccgcggg cgagcaggtt atcgataata tatttaccga taaacccggt agcgcccgtc
     9001 accgcgacag tttgattcat gccagggatt cctgcacatg aaggcttgca gcataagcct
     9061 tctgataacg ttttttcgct tccgcacggg caggcttgcc ggaggatgtt cggggaatac
     9121 tgtggggcgg caacagatcg atagccgcgg ttacgccaaa ttcgctttgg atccgtgccg
     9181 ccagcgcgtg gataagctgc ccgcgacgtt cttcgtcgct gatccgacac tggatctgca
     9241 aaatgatttt ttcctggacg gtaacaaaag caatcgcatc gccagaatga atttccggtt
     9301 cttgttccgc aatatattca atatcctgcg gccagatatt acggccacga ataataatca
     9361 gatctttaat acgtccggtg acatacagat aaccgtccag cagataaccg aggtcgccgg
     9421 tgtctaacca gcccgtcgcg gcaatctcgt cttgcgaaac ctggtcgcca aagtaaccgc
     9481 tcatcaggct ggggccggag atgcaaatat ggcctaccac acgttccgcg accggcatac
     9541 ctgcttcatt gcggatttca atgccatgtt ccggcaacgc tttgccgcag ttgacgaaag
     9601 tcgatacggc gcgtgtttca gcacccggcg cgacggcttt gccctgatat tctaggatgt
     9661 cgcgatccac ttcgttaacc acaaccccag agccttcatc agagaagctg acagccagcg
     9721 cattttctgc cagtccgtag cacggcatga acgttttatc gtcaaagtta acctgacgga
     9781 aacattcagc gaattgatgg agttgttctg cggagatcgg ctcagcacca ataccagcga
     9841 cgcgccagca ggaaagatcc agttcagcga gatctttttc attcacgcgg cgttggcaca
     9901 attcatagcc aaacggcggc gcaacggaaa cggtgccgcg atttttactg atcaatttaa
     9961 gccattgcaa aggacgcatg gcaaaatcct gagtgcgcaa ataatctact gaaagttgcg
    10021 tggcgacggg ggtcaggaga aagccgacca gtcccatatc atggtagaaa ggcagccagg
    10081 agacgcagcg gtcgccaggg cgtaatttaa tgccatcgtg gcttatagca cgtagattag
    10141 ccatcacttc gcgatgggtg ataatgacgc cacggggaaa acgggtgctg ccggaggtgt
    10201 actggaggta ggcgatatcg ttcggaactg gacgctggag cgcaacatcg gcttccggta
    10261 atgccttaaa ccaggcgtgg cttaaaacat gtaattcggg gttgttatgc gtcgcggcat
    10321 tgaccagtgg caaccactca tcaccagtga taatggctgc gggctggcaa cttgccagta
    10381 aaccctggag tttggcgctc caggaatccc gctgaccgac gcccattgga atcgccaacg
    10441 ggacggcgac taagccagca tactgacagg caaaaaaagc ctctacaaac ccgctactcg
    10501 tttcggcaat cagcgccacg cgttcgcctt ttttcaggtt cagcgataac aaccgcttcg
    10561 caccagcttc ggcacgcgtt tttaatgtct gatattccag ttgatcttca agttggcaac
    10621 gtctgtcata aaaattcatt ccggcgctac tcagagcggc gtagtccaaa gcatcaacca
    10681 gcgttggaaa atcggcatag cgcataggta gggaatgcgt aaagatttta ttagacatat
    10741 acaccacgaa aaaattggat atcaaa
//
LOCUS       DAPGWD010000144        10663 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPGWD010000144 DAPGWD010000000
VERSION     DAPGWD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.145"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            152..1219
                     /gene="pilL"
                     /locus_tag="RHX88_003560"
     CDS             152..1219
                     /gene="pilL"
                     /locus_tag="RHX88_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HDV2232208.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAHPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            1219..1656
                     /gene="pilM"
                     /locus_tag="RHX88_003561"
     CDS             1219..1656
                     /gene="pilM"
                     /locus_tag="RHX88_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HDV2232209.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            1670..3352
                     /locus_tag="RHX88_003562"
     CDS             1670..3352
                     /locus_tag="RHX88_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HDV2232210.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            3345..4640
                     /gene="pilO2"
                     /locus_tag="RHX88_003563"
     CDS             3345..4640
                     /gene="pilO2"
                     /locus_tag="RHX88_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HDV2232211.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAASAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            4627..5079
                     /gene="pilP"
                     /locus_tag="RHX88_003564"
     CDS             4627..5079
                     /gene="pilP"
                     /locus_tag="RHX88_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HDV2232212.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            5090..6643
                     /gene="tadA"
                     /locus_tag="RHX88_003565"
     CDS             5090..6643
                     /gene="tadA"
                     /locus_tag="RHX88_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDV2232213.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            6656..7753
                     /locus_tag="RHX88_003566"
     CDS             6656..7753
                     /locus_tag="RHX88_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDV2232214.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            7771..8385
                     /locus_tag="RHX88_003567"
     CDS             7771..8385
                     /locus_tag="RHX88_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HDV2232215.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVL
                     GSLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            8395..8955
                     /locus_tag="RHX88_003568"
     CDS             8395..8955
                     /locus_tag="RHX88_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDV2232216.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            8940..9596
                     /locus_tag="RHX88_003569"
     CDS             8940..9596
                     /locus_tag="RHX88_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDV2232217.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            9596..>10663
                     /gene="pilV"
                     /locus_tag="RHX88_003570"
     CDS             9596..>10663
                     /gene="pilV"
                     /locus_tag="RHX88_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HDV2232218.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITAGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGAIL"
BASE COUNT         3049 a         2537 c         2458 g         2619 t
ORIGIN      
        1 gtatgaaaga ttatcaattc cttttgtata tctcttattt cagtggtctc tcccgcattt
       61 gcattcggac ggaatgacag tcgtcattcg tcctctccag ccgcctcaca ccaattaaca
      121 aacacgtata tacgaacacg gagtctccgt tatgaaaaaa aactatctga cacttatact
      181 gccgtcatta ttgtccggct gtgccggaat ttccggcagc aataacgtgg caccatcaga
      241 tacgcctgct ctggtttttg ttgacggaca aatcagtgag agtgctggcg ttctggcact
      301 tacgcaacaa cgtctttctc cacctaaaat aactcccccc ccgagagtca gcgtgccaga
      361 gacagtaaaa acagcacatc cagtcagcga atcccgaccg ttaccttcac caacaataag
      421 tcagaatttc tcccggttaa tcattacagg caagcctggt agcgcccctg taacactatc
      481 gccgacggcc cgtaaccgga ccgtcagtca gtggataaaa acattactac cggaaggatg
      541 gaaattcaga caagagaatg cagccacgcc caagctgaat accaaactgg tttcatggtc
      601 tgccaatgat cagtggaccc gttctctgaa tcggttgctg gaagaacaac acctatgggg
      661 ccatctggac tgggataata aaacgttgac cgtcacaaca tcagctgctg caccggcaca
      721 ggacacggca accactatct cgccagcagc catggtaacc acatccactt cccctgaatc
      781 tccaacaaca gcaaatagcc agaacaaacc acgaaatcca ttcagaggaa acagcgtatc
      841 ttcgtcaacc cccgccgcca ccccgactcc aaccggctcc acagtcaagt ctatcccgct
      901 catgaccgga acgcctgtta aaccggtatc tcagggtaaa gagtggcgtg cgccggcagg
      961 caccacatta agagagaaca ttatcaagtg ggcagaagaa acaaaatgcg aaagcatggc
     1021 atcaacgcac tggatggtca tatggccact ttctgtaacc gattaccgtc tggatgcgcc
     1081 tttaatgttc cggggatcat ttgaatcagc catggagcag gtatttgagt tatatcggac
     1141 tgcgcaaaaa cctttatacg cacaggcttc tcgcatgcag tgcatcatta cagtttctga
     1201 tacaagggat ggtcgctaat gggatggctg gtcatggccg tcggcctgac cattctgatt
     1261 atcactggta gctaccagaa tcagaagatg tctgaaacca caaacgccca gcaatatgca
     1321 agtgcgtctg tatgggcaag tcagatactg atgattgcta acagaatcaa tgatatcaga
     1381 tacgtatcag gccagcagga cggagtaatt tcctcagata aactggctct ccctgtcact
     1441 ccggactccc gaattaaaca tcagttacaa caggggcgtc tgtgggtatg gatgcctgaa
     1501 caaccaggtc ttgttgaaac tttgcgttca aaaagtcgtg gttctgcact aatagggatc
     1561 ttccaaaacg ggcaactcac ctggctatca ggaactgcaa ccggtctgac acccccggca
     1621 ggcattactg ctggttctgt cgtctacgtt aactaatctg gatatattca tgaaaaaatc
     1681 acaccagcgt tcaatgaagc tggcggtgct cccctgcatg atcgccgtcg ctctttccat
     1741 ctccggatgt actttcagcg aaatcaacaa aatgcagaaa aaagcacagg aagactcagc
     1801 acatgcacgg gaaaaggtat cagccctttc ggcccgtaaa tcgcaggctc ttacctggct
     1861 cgataatcaa tggataaacc ctgttccggt cgctcaggta tcaagagaga aaaaacaaac
     1921 agctccggcc tgctacatca cgcaggcaag aaaaggagag atcactctgc aggaactggg
     1981 gcaacgtatt actgccgtat gcggcatccc tgtgatcatc acgcctgacg cagccaattc
     2041 aactcttgaa ggaggcgcta cccgccagat gacaggaaca ctaccagcac cagatgaaaa
     2101 tgggcgtcta ccgttaagca gcctgggcag cacaacaatg actacctcca ctcagccatt
     2161 aacgctgaat aacctcatgt ggcagggaga tatcaatggt cttctggatc tgatggccag
     2221 ccggagtggt ctgtactggc gcatggataa tggtcggatt gtattctatc tgactgaaac
     2281 cagaacgtat ccacttcata tgctgaacac caaaaccagc agcagttcca gtgtcagctc
     2341 tggctcaaca agcacaatgg gggcaacagg aggccaggat aactcagcat ccggtgatgc
     2401 aacgtcctct cagagcacaa ccgttggtca ggaatacgat ctgtatgaag acatccggaa
     2461 aactattgaa gcaatgctga caccagaaaa aggccgttac tggttatctg catcgagctc
     2521 aacgctgact gtcactgata ctccagctgt ccaggaagcc gtcgcacgat atgtggacga
     2581 acaaaacagt attatgaacc gccaggtagc cctgaacgta caggttctga gcgtcagcaa
     2641 taccagaaac gaacagttcg gtctggactg gaaccttgtt tataaatcgc tacattccgc
     2701 cggagcaacg ttgaacaatg caagcggaga ttttacaggc gctacatctg caggcgtatc
     2761 aattctggat acggcaacag ggaatgccgc caaattcagc ggttccagtc ttctgattaa
     2821 agcgctgagt gaacagggcg atgtcagtgt tgtgacttca caagaaagca ctgtcacaaa
     2881 cctgacgccg gtacctatcc agatggcaga tcagacggtt tacgtcgccc agtcagcaac
     2941 aacaacgact acggatgtag gagcaacaac aacattaacg ccgggcatga tcaccaccgg
     3001 attcaatatg accctgctgc ctttaattca gaaaacgggc aatctccagt tgcagatgaa
     3061 ttttaatctg tcagatcccc caacaatccg tagctttacg tcaaaagacg gaaacagtta
     3121 catcgaaatg ccgtatacca aactgcgttc actgagccag aaggtcaatc tgaaagaagg
     3181 gcaatcactt gtcgttactg gtttcgatca gaacaatacg acgacaagta aagccggtac
     3241 gtttacgcca gcaaatccat tatttggtgg ttcacaaacc gggaaaaatg aacgcagcac
     3301 gcttgtaatc atcattaccc cgactttccc gtcaggaggc aacaatggct gatgaagata
     3361 tcaacccggt tgtacttctc gcagatccta aagtaaatca tcgcgtgtgg gcagcctgtc
     3421 ttaaatggag tcctgtcgta aaaaaacagc gagtcccttc tcatcagaaa cacaagcccc
     3481 atgtaaaatc cagacggctt acctctctga aagtgactgt aggcagcaga agttcccgtg
     3541 ggaagatcag ccgcattacc ggcacaggaa tactggccag gccggagcgc aatcattatt
     3601 tttcccttgc tctggctttc tgctcatggg tgagaaatgg atacggcgtc ttccggtaca
     3661 gcgataagga gcttttattc cttgcctcca ttaatggcca gcctgccgtt atggctgatc
     3721 tgtcaggaaa tgatgctgat gttgctcaaa aggtcagtct ttttctgacg atgaacgaag
     3781 agccccccga aaaatggcag gtcgtatctc ctctggaaca tcctgataac tgggaaagta
     3841 ttattacccg gttatcatca gcagatctcc gccgttgcaa acttactgtc ggcaatcgca
     3901 gcaaattcac actgcctgcc gtactttttt tagtagcagc cagcgcaggc actgtattct
     3961 ggatgactca gccagagccg gatgtcgtac cgacgcccga ggagatagct gcacgggcaa
     4021 gattgcagtt taaaaagccc gatccaccgc cagaactccc gcatccatgg gcatcacaac
     4081 ctgtaatcag tgattttctg aaagcctgcg cggaccttcg aaaaccatca ccggtcgcgc
     4141 tagaaggctg gaaacttacc ggtggaacat gtactcccga gacattcaca ctgatttacg
     4201 aacgacagcc aggaggaact attgaaggct ttctggcaag aagcaaagaa atctttaatg
     4261 ttattcctga ctttaacctt aaagatggtg cacgcctggc ttctgtaacc cggccactgc
     4321 cttccctgcc ccgcagggat gaggcagtcc ctaccccctc agaacaactg atgcgggtgt
     4381 tcacgtggtt tcagaaaaaa cagttaacgc cggccattaa tgaaatagcc ataccggaac
     4441 cattaccagg taacgatggt gaaccagccc ctgttcagaa atggaaagaa tatcagttca
     4501 gtttatcaac gcctgtaaat cctgatgagt tgttcccttt atttcaggat accggcgttc
     4561 gtctgagcaa tatccatttt gaattaaacg gcggtacttt cagttattcc tcagaaggtc
     4621 atatttatgc gtccaggtaa attactcatt attccatcca tatttttctt ctccggtttt
     4681 tcatttgcaa caacacaacc tcttgtcaca ataggagaac ttgaagcaca acaaaaccgg
     4741 aatattcttt tacaggcaaa agttcagggc gcacagctac agaaacaact cgaagaatca
     4801 gatgttactt catcatctga aactgtttca ggtttctctg gtgtgactgc ctctttgcca
     4861 tcagtttcag agcagccgac atctaaaaac agaaaggaat tgcccgttat tatggaaata
     4921 aacggcaaag ataaacgtct taatgccgtt cttcgcatgg ctgatggacg tcagacaagc
     4981 gtcaccactg gcagccagct tccaggcaca tccgtcacag tgaagtcgat ctcattgtct
     5041 ggcgtcacgt taagtgatgg caccaccctt actttctgag taacgtgata tggataaata
     5101 taatctgaag gatgctctcc tttttatatc caggggagac acccatgaaa ttcttataga
     5161 aacaaaccag cggacaaggc ctgacgtaca aagtaatctc caggagctgc taaaattata
     5221 tccggatatc aacccaaaag tagtctcgtt gtcggagtta caggaggcag gtcaggatga
     5281 gaaaaacaga ggcccgaaag aacgaattat tcatctgaaa gaccttgctg atgtcagtga
     5341 aagtgagaag aaagttctat cttattttga aactgccaga aaacttggtg cttcagatat
     5401 tcatttctta atttcagagt ctattttcaa agttcgcatg agaatattcg gtgaactgca
     5461 gactgtagat gaagatcaac cagcactggg ctattcactt tgcgcaacag ccattctgtc
     5521 aatggctgat gtcactgaaa ccagtttctt ccctcaaagg gagcaggatg cgcgcctgtc
     5581 cccacaatta atgcgaaaaa taggaatatt tggtgcacgc tacagtcaca gacctacggg
     5641 agacggtctg attgcagtta tgcgcctgat acccgatgac ggagataaag ttcccacgtt
     5701 taaacaactg ggctttatcc cggaacaaat ccggttgctg aacataatgc tgcgccgccc
     5761 ggaaggaaaa atagtcctgt caggtccaac cggttccggt aaatcgacaa cgttacgcag
     5821 cgcctgccgt gtatatcttg acgataatca ggggcgacac ctgctaacaa tcgaagatcc
     5881 gctggaagga cagatactgg gagcaacaca gactccgatt atctgcgaca aatccgacga
     5941 agatgccgtc aaactggcat ggagtcgggc tatttcatcg gcaatgcgac ttgaccctga
     6001 tgccatcatg gaaggggaaa tgcgcgatct tatctcgatg atgtcaacca cctacgcggc
     6061 acagaccggg cacatagttc tgacgacatt acatacaaac tcagctctgg gtataccgga
     6121 gcgaatgatt acgatgggta tgaatgccga tcttatttgc gatgcgcaat tactgattgg
     6181 gatgatcagc cagcgccttg taccaactct gtgtccctcc tgccgtattc catgggaaaa
     6241 gcgagcacct gaacttagcg acgacgagcg tgattacctt gagcggcact gcaataaaga
     6301 ttcactctgc agtacagata atatctggtt ccgaaatcat cacggatgct ctgaatgtaa
     6361 tcacgacgta atcattaacg gcagaaagcg cggtgaaata ggtaaaggcc tcaccggcag
     6421 gactgtaatt gctgaggtga tagaacctga taaccgctta tttcagattc tgaaaacacg
     6481 cggcaaagtt gcagcacgta aatactggct ggaaaacatg aaaggcatca gtcgtgtaga
     6541 gcatcttctt cgccgtatca atgaaggtct ggtagaccct cttgaagccg acagaatcat
     6601 ccctctggac gaagacgagc gcctcagcat cgatgatgtc taacaggaga acaacatgcg
     6661 tgaaatgaat ttcagtcaac ggctgaggcg tttcattgtc agaaaaacgt tcagcgcccc
     6721 ctacagggtg cagttttacg aagccctgcg ttttctgctg gaaaataaac agccattaaa
     6781 gaccgcactg gaacagatgc gggatgcctg gacagacttt ggacgaaaat ggcatccctt
     6841 tgctgaactg gccacagact gcattgagtc tcttcgtgaa aacagtggcg aaaactctct
     6901 ggaatatact cttagtttat gggttcctca ggaagaggcc gcagtaatca gcgccgggat
     6961 aagaagtggt tcaattgtgg atgcactgca attcgccacc actcttacag atgcaaaaga
     7021 acaaatccat caggctatat ggcaaatggc tatttatccg gtgggattgt taatcatgat
     7081 gacggggaca ttatatgttc tgaatacaga gctaatacct gaactgagca aaataagctc
     7141 ccccgattca tggagtggtg ctcttggttt tctttatgga ttatctgttt ttgtcgataa
     7201 ttacggcgct atatgtgccg ttctttttgc agtgatcaca ggtttaatat catggtctct
     7261 gccaaactgg aaaagccctg acagtgttcg tacttttgcc gacaagataa tgccatggag
     7321 tatttatcag gatatacagg gagcaacatt tctcctgaat atggctgcac ttctgaaagc
     7381 caaaatgacg acgctcaact cgctaaacat tcttcaggag tttgcatcac catggttaag
     7441 cacacgtctt gacagcatta tttaccgcgt ccgccagggg gatcatcttg gactggcttt
     7501 gcgtcagtgc ggttatcagt tcccatccag ggaagcagcc aacttcctgt ctttattaca
     7561 gggagatggc gcaacagagc taatcagcaa ttatggtcaa cgatggctta gtcaaacact
     7621 tcaacgtgta aagaaacggg caaacgtgat tcgtttaatt atgctgatat ttcttgtcat
     7681 gtctctcatg ttactggttt ttgccattat ggatattcag tctattagtg acaacagcat
     7741 gggtaacttt taaacaaaac gaggatattt atgttagtag aaaatattaa taccacttta
     7801 actggaaata ataagaagaa tgagcctcat gataaaggat gggcaatttt ggagcaggga
     7861 acaatagcgc tcgttgttct ttttgttata gtcgttgttc tgggtagttt gtatgcgttg
     7921 cgtacaagag ccaatgtggc aactgaaacc gcaaatatac aaacaattat cacaagtgcc
     7981 cagagcctgc taaaaggtag cgatggatat acatttacca gcagtgccaa aatgacaggt
     8041 gccctcattc agatgggtgt cattccctct gggatgaccg tacagggcga caaaacatct
     8101 ggtacagcaa cgctctataa cgcatggggc ggtgctgtaa ctgttgcccc ggcttcaaca
     8161 tcagggttca acaacggttt taccgttacc tatgataaag tcccacagga tgcctgtatc
     8221 cagattgcca cacgaatcag taaaactggt ttaactaacg gcattaccct gaacagtact
     8281 gctcacagtg atggaaaagt cactactgaa gaagccagta cgcaatgcaa ggcagataat
     8341 ggcagcactg gtacaaacaa acttatcttc accatcaatg gttaaggagt tttaatgtca
     8401 gggggtacag tacccctcag acttatttca ttaatcctgc ttttttctct tattccaccc
     8461 gcccgagctt catacgtagc tcccaaacag atctggacca acaagtggga caattgcttt
     8521 gccgctgccg gcgcgcgcta tcaaattgaa ccattattat tgaaggctat ttctgccggg
     8581 gaatcttcgt tgaggccggg cgcaattaat ataaataagg acaaaaaaac aggtaaagcc
     8641 agtagcacgg attatgggtt gatgcaaata aactcaaccc atattccaaa gctgataaat
     8701 atgggcgtaa taaaaaagtc tgaggattta attaccaaac catgccttaa tatccatata
     8761 ggaagctgga tactggcaag acattttcag atatgtggcg tcagctggaa ttgccttggg
     8821 tcatacaatg ccggattccg taaggacaga catgaaacaa gagagcaata tgccaataaa
     8881 atatggagaa tttatcgtga catgaaaggg atatgtcttc ccggacaggg aggcaggcaa
     8941 tgcagacaat cgtaggcata agtctatccc ctgtttatat actgccattg atgttcacat
     9001 tttccattat tggacgaact ctgctattca gagttcgtta ttttttatct gatacgggac
     9061 atctctggta taagacacac cctgctgttc tgtcaggaat atggctatac agtatagctg
     9121 tggcgttaat tattttatca tcatcaccac tgctctaccg tattcatgct gtccttattc
     9181 tttcgttcat actgcaaatg gcggtaacgg atgcactgac aggtttatta ccaggaacat
     9241 tcacccgacg ttttttaatt gcaggaatgc tatcacaaat cacgactgat atatggtggt
     9301 tccgtacaac agaatttgcc actgctgcaa ttgtcttatt ctgccttcat aaattagtta
     9361 accgccatcg tcttaatatt ggcactggag acctgtggct tattgcaggt attaccgcat
     9421 ggtcagggtt atataacgcc atatggtgtg tgttgctggg cacaggagga tttgtcttat
     9481 ggcattcaac atggtgcata aaagggcata aagaaggccc tctgggtcca tggctatgtt
     9541 ttggccatgt ccttttgctg ctggataatc tttatcaacc actatgggta atctgatgaa
     9601 aaaatatgac cgaggctggg catcgctgga aactggcgcg gctttactga ttgttatgct
     9661 cctgattgcc tggggagcgg gcatatggca ggactatatt caaacaaaag gatggcaaac
     9721 tgaagcacgt cttgtcagca actggaccag tgcggcccgc tcttatatag ggaaaaacta
     9781 cacgactctc cagggcagta gtaccacaac aactcctgcc gttatcacga caaccatgct
     9841 gaaaaatacc ggctttttgt ccagcgggtt tactgagaca aacagcgagg ggcagcggtt
     9901 acaggcatat gtggttcgaa acgcccaaaa cccggaatta ctacaggcaa tggttgtatc
     9961 cagtggtggc acaccttatc cagtgaaagc acttatccag atggctaagg atattaccgc
    10021 tggtcttggt ggatatatcc aggacggcaa aacagccaca ggtgcattac gttcctggtc
    10081 agtagcttta agtaattatg gtgccaaaag cggtaacggg catattgccg tattgttatc
    10141 gacagatgaa cttagtggtg cagctgagga cactgatcgt ctttacagat tccaggtcaa
    10201 tggtcgccct gacttaaaca aaatgcacac ggccattgat atgggatcaa ataacctgaa
    10261 taacgttggg gcagtaaatg cccagacagg taatttcagc ggcaatgtga atggtgtaaa
    10321 tggcactttc agcggtcagg taaaaggcaa tagcggaaac tttgacgtaa atgtcaccgc
    10381 tggcggtgat atcagaagta ataatggttg gttaattact cgtaacagta aaggctggct
    10441 caatgaaact cacggtggcg gattttatat gtccgatgga tcatgggttc gaagtgtaaa
    10501 caacaagggc atctataccg gcggtcaggt gaaaggcggt actgttcggg ctgatggtcg
    10561 cctttatact ggtgaatact tacaactgga aagaactgcc gttgctggcg catcatgttc
    10621 gcctaacggc cttgtaggcc gcgataatac aggggcaata ctt
//
LOCUS       DAPGWD010000145        10635 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPGWD010000145 DAPGWD010000000
VERSION     DAPGWD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.146"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            151..1518
                     /gene="dcuC"
                     /locus_tag="RHX88_003571"
     CDS             151..1518
                     /gene="dcuC"
                     /locus_tag="RHX88_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV2232219.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1561..2058)
                     /gene="sspB"
                     /locus_tag="RHX88_003572"
     CDS             complement(1561..2058)
                     /gene="sspB"
                     /locus_tag="RHX88_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDV2232220.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2064..2702)
                     /gene="sspA"
                     /locus_tag="RHX88_003573"
     CDS             complement(2064..2702)
                     /gene="sspA"
                     /locus_tag="RHX88_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDV2232221.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3097..3489)
                     /gene="rpsI"
                     /locus_tag="RHX88_003574"
     CDS             complement(3097..3489)
                     /gene="rpsI"
                     /locus_tag="RHX88_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDV2232222.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3505..3933)
                     /gene="rplM"
                     /locus_tag="RHX88_003575"
     CDS             complement(3505..3933)
                     /gene="rplM"
                     /locus_tag="RHX88_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDV2232223.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4152..5279)
                     /gene="zapE"
                     /locus_tag="RHX88_003576"
     CDS             complement(4152..5279)
                     /gene="zapE"
                     /locus_tag="RHX88_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDV2232224.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5473..5871
                     /locus_tag="RHX88_003577"
     CDS             5473..5871
                     /locus_tag="RHX88_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDV2232225.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6025..7392
                     /gene="degQ"
                     /locus_tag="RHX88_003578"
     CDS             6025..7392
                     /gene="degQ"
                     /locus_tag="RHX88_003578"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDV2232226.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7482..8549
                     /gene="degS"
                     /locus_tag="RHX88_003579"
     CDS             7482..8549
                     /gene="degS"
                     /locus_tag="RHX88_003579"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDV2232227.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8612..9550)
                     /gene="mdh"
                     /locus_tag="RHX88_003580"
     CDS             complement(8612..9550)
                     /gene="mdh"
                     /locus_tag="RHX88_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV2232228.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            9985..10455
                     /gene="argR"
                     /locus_tag="RHX88_003581"
     CDS             9985..10455
                     /gene="argR"
                     /locus_tag="RHX88_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDV2232229.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2628 a         2700 c         2638 g         2669 t
ORIGIN      
        1 taagcgcata ttttttaatg aaaaaccacc tcacctacaa ttaaaaacac gacatccgca
       61 ccataaatag acttgcaaaa aatataacat cattgttttc aatctgccgt ttatgggatt
      121 gaccgttttc ttttgacacg gagttcaaca atgttcggca taattatatc tgtcatcgta
      181 ttaattacga tgggctattt gatcctgaaa aactacaaac ctcaggtggt gctggctgcc
      241 gcaggtatct tcctgatgat gtgcggtgtc tggttagggt tcggtggtgt actcgatccc
      301 gccaaaagca gcggctactt gatcgtcgat atttataatg aaatcctgcg catgctgtcc
      361 aaccgcattg ccggattggg gctgtcgatt atggcggtgg gcggttatgc ccgctacatg
      421 gagcgcacag gagccagtcg cgcgatggtg agcttgctaa gccgcccgtt aaaactcatt
      481 cgctcgccgt atattattct gtcggcaact tacgtcatcg gccaaatcat ggcgcagttt
      541 attaccagcg cctccggcct gggtatgttg ctgatggtca ccttatttcc gacgctggtg
      601 agtctgggag taagtcgcct ctctgcggtg gcggttatcg caaccacgat gtccattgag
      661 tgggggattc tggaaacgaa ctccattttt gcagcccagg tcgcgggaat gaaaattgcc
      721 acttacttct tccactacca gcttccggtc gcctcttgcg tcattatctc ggtggcgatc
      781 tcccactttt tcgtgcaacg cgcttttgac aaaaaagata aaaatatcaa tcacgaacag
      841 gcagagctaa aagctctcga taatgtcccg ccgctctatt acgccatttt acctgtgatg
      901 ccgttaatct tgatgctcgg ctcgctgttc ctcgcccaca tcgggctgat gcagtcagaa
      961 ctgcatctgg tggtggtgat gttactgagt ttgacggtga cgatgtttgt tgagttcttc
     1021 cgcaagcata acttgcgcga aacaatggac gatgtgcagg cgttttttga cggcatgggt
     1081 acgcagtttg ccaacgtggt aacgctggtg gtcgcgggtg aaatatttgc gaaaggctta
     1141 acgacgattg gcactgttga tgcggttatc aggggtgcgg agcattctgg tctgggcggt
     1201 attggcgtga tgattattat ggcgctagtc attgccattt gtgccattgt gatgggctct
     1261 ggcaatgcgc cgtttatgtc atttgccagt cttattccga atatcgcagc cggactacat
     1321 gtaccagcgg ttgtaatgat tatgccgatg cattttgcca cgacgctagc gcgcgcggtt
     1381 tcgccgatta ctgcggtggt ggtcgttacg tcaggaattg caggcgtttc gccttttgcg
     1441 gtggtgaagc gaacagcgat ccccatggca gtcggtttcg tggtgaatat gattgccaca
     1501 atcacgctat tttattaagc cattaaaaga caaaacaggc caccagggcc tgtttttgta
     1561 ttacttcaca acgcgtaatg ccggtcgacc accgcgtggt ggctgcggag gttcatcgtc
     1621 aggatgagtg tcatcatcgt gatctggctt gtcgccatca ataaccgaca taacggtttc
     1681 gttgtctgcc gatgcctctt catcattcat gatgctggta tcttcatcgt aggcagcttc
     1741 aggctcaaac atcgtgcctg cgccattttc acgggcgtag atagccagca cggcagccag
     1801 cggcacagaa acctgacgcg gaatgccgcc aaagcgcgcg ttaaagcgta cctcatcatt
     1861 cgccagttcc agattgccga cagcacgcgg cgcaatgttg agtacgattt gcccgtcacg
     1921 cgcatattcc ataggaacct gcacgccagg gagcgtcaca tccaccacca agtgcggcgt
     1981 gagctggtta tccagcaacc actcatagaa tgcacgcagc agatagggac gacgtggtgt
     2041 tagctgtgac aaatccatac agattaactc cggcccagac gcatttcacg ttctgcttca
     2101 gttaaagaag caaggaaaga gtcacgctca aagacgcggg tcatatagcc tttcagctct
     2161 ttcgcacccg ggccgctgaa ctcgatgccc agttgcggca gacgccacag cagcggagca
     2221 agatagcaat cgaccaggct gaactcatcg ctcaggaagt acggcttctg accgaagacc
     2281 ggcgcaatcg ccagcagttc ttcgcgcagt tgcttacgtg cggcatctgc ttcagaagct
     2341 gaaccgttga tgatggtgtt catcagcgtg taccagtctt tttcgatgcg atgcatgtac
     2401 agacggcttt caccgcgagc taccgggtaa acaggcatca gtggcggatg cgggaaacgc
     2461 tcatccagat attccataat gatgcgagat tcccacaggg tcagctcacg atccaccagg
     2521 gtcggaacgc tctgattcgg gttgaggtca atcagatcct gaggcggatt gtccttttcc
     2581 acgtgttcga tctcgaaact tacacctttc tcagccagca caatgcggac ctgatggcta
     2641 tagatgtcag taggaccgga aaacagcgtc attaccgaac gtttgttggc agcgacagcc
     2701 atgaaaacct ccaggtatag tcagaatttt tactgctacc agccaccagg tggccagtca
     2761 gaagttgtgt tacccagtaa ggaacgactc tctttgttcg aaaatcaaac aaaaaatgag
     2821 caatacccga catttgggca gaaaattgga tgatagttta ccagattttg cgacctttgt
     2881 ggtgagtcga tgccggaaat ggggaaaaag agatgcgctt tagtctgaaa tagttgactt
     2941 agtcccttat tggcgatgtg gtttttgttt tgcctgtctg tcagacggta gcaaaaagca
     3001 actttccaat ttttacgctg attcagaatt taaccataaa aaaacccgcc gaagcgggtt
     3061 ttttcgcaaa ttgttttctg ccggagcaga agccaattaa cgtttggaga actgcggacg
     3121 acgacgtgct ttacgcagac cgactttctt acgttcaacc tgacgagcgt cacgagtaac
     3181 gaagccagct ttacgcagtt cagaacgcag ggactcgtcg tattccatca gagcgcgggt
     3241 gataccgtga cggatcgcac cagcctgacc agagatacca ccacctttaa cggtgatgta
     3301 caggtccagt ttctcaacca tgtcgaccag ttccagcggc tgacgaacta ccatgcgagc
     3361 agtttcacga ccgaagtact gttccagaga acgttggttg attacgattt taccgttgcc
     3421 cggtttgatg aaaacgcgag ctgcggaact tttgcggcga ccagtgccgt agtattgatt
     3481 ttcagccatt gcctataatc ccgattagat gtcaagaact tgcggttgct gtgccgcgtg
     3541 gttgtgctcg ttacccgcgt aaactttcag tttacggaac atagcacgac ccagcgggcc
     3601 ttttggcaac atgcctttaa ccgcgatttc aatcacacgc tcaggacggc gagcaatcat
     3661 ctcttcaaag gtcgcttgtt tgataccacc gatgtggccg gtgtggtgat agtacacttt
     3721 gtcagtacgc ttgttgccgg ttacagcaac tttgtcagcg ttcagaacga tgatgtaatc
     3781 accggtatct acgtgcggag tgtattccgc tttgtgctta ccgcgcaggc gacgagccag
     3841 ttcagtagcc agacggccca gagttttacc ggtcgcgtca acaacatacc agtcgcgttt
     3901 tacggtttct ggtttagctg taaaagtttt cattaaaagc ttacccaata aatagttaca
     3961 cgttggtgaa cacccaaacg tcttcaattg ttgaggttca cacgacaaag tccggcaaac
     4021 ctaccccttc gaatagccta tgccagcaca caaaaagttt tgggaaaaaa actttcttgt
     4081 aacgtggggt cgcaggatta tagagaagtc ggggtcaaag atcgacccct ttttgtgatt
     4141 tgtgacaggt tttaacccgc caaatgctcg cgcttcagat actcttcgct ttgcatctct
     4201 tgcagacgtg acaggcaacg ctggaactca aacttcagcc gatcgccctg ataaatttca
     4261 tacagcggca cttctgcact caccactaat ttgacatggc gctcgtaaaa ctcatccacc
     4321 agcgcaataa agcgccgcgc ttcgctctcc atcaaccgcg tcataactgg tacatcaaac
     4381 aacatgaccg tatgaaagag acgtgagagc gcaatatagt catgctgact gcgggcgtcg
     4441 acgcacagcg tagtaaaaga gaccgccagc gtctggttct cgacgcccat tgtcgccaat
     4501 ggccgatggt tgatttctaa cgtcggtgaa ttttctcgtt tcgcccccgc cagcgccaac
     4561 catagtttat ccatctgcgc ccgggtttca tcgttgagtg gcgaaagcca cagatgcgcc
     4621 tgagtgagtg tacgcagtcg ataatcaaca ccagcgtcca cgttcattac atcacaatgc
     4681 tgtttaatgg catcgattgc aggcagaaaa cgcgcacgtt gcaggccatt tcgataaagt
     4741 tcgtccggcg gaatatttga cgtcgctacc agggtaatac cgcgggtgaa cagggctttc
     4801 atcagaccgc caagtagcat ggcgtcggta atatcagaaa caaaaaattc gtcaaaacag
     4861 agcacgtcag tttcggcttt aaagcgatcg gcaataattt ccagcggatc ggtctgcccc
     4921 tgtaaggcag ttagctcttc atgcacccgc agcataaaac ggtgaaagtg caggcgctgt
     4981 ttccgctctc ccggcaggct ttgatagaaa aggtccatca gccaggtttt cccgcgtcct
     5041 acaccgcccc acatatataa gccacgcact ggcgtatgct ttgtgtcttc gcgtttaccc
     5101 cacagcttac cgacccgcgc cattagccca ctcgtcctgg gggctggtgg cgtgctattg
     5161 atgagttcct gataaataat ttccaggcgg ctgacggcct ctttttgaac gtcgtcgggt
     5221 tgatggctgc cttcattgag cgccttcagg tattgcgatg ttggggtaac gctttgcatg
     5281 atcttattgt tattccttga ataatcggtg cgccgttgtt cacggttgac gaaaaaaagg
     5341 ccgttctaca ctacgcgata tgcagtcggg attccacttc tgtggaatta acggttatag
     5401 tggcataatc agccgcaggc atggagcctg aagccaacac cctacggaaa caaaagacaa
     5461 cgggagatgt tcatgacctg ggaatatgcg ctaattgggt tagtcgtcgg catcattatt
     5521 ggtgctgtgg ccatgcgttt tggtaatcgt aaactacgcc agcaacaggc gttgcagtac
     5581 gaactggaaa agaataaagc tgaactggac gagtatcgcg aagagctggt tagccacttt
     5641 gcccgcagcg cggaattact ggataccatg gcgcacgact atcgccagct gtatcagcac
     5701 atggcgaaaa gctccagcag cctgctgccg gaactgtctg ctgaagcaaa cccgttccgt
     5761 aatcgtctgg cagaatctga agccagcaac gatcaggcac cggtgcagat gcctcgcgac
     5821 tattctgaag gcgcatccgg cctgctgcgt actggcgcga agcgcgacta atttattttt
     5881 cgggcgcagc cattgcgccc tcctcttctc tccctccccg actatcattt aatctggtgt
     5941 ctcattgtta gccgtctgaa aattcaataa catcaaactg ttttgaatct cttttcttat
     6001 cattcaggta cgagagcagg aataatgaaa aaacaaaccc agctgttgag tgcattagcg
     6061 ttaagtgtcg ggttaactct ctcggcgtca tttcaggccg tcgcgtcgat tccaggccag
     6121 gttgccgatc aggcccctct ccccagtctg gctccaatgc tggaaaaagt gcttccggca
     6181 gtggtgagcg tacgggtgga aggaacggcc agtcagggac agaaaatccc ggaagaattc
     6241 aaaaagtttt ttggtgatga tttaccggat caacctgcac aacccttcga aggtttaggt
     6301 tccggtgtca tcatcaacgc cagtaaaggc tatgtgctga ccaacaacca tgtgattaat
     6361 caggcacaga aaatcagtat tcagctcaat gatgggcgcg agtttgatgc aaaactgatt
     6421 ggtagcgatg accagagcga tatcgccctg ttacaaattc aaaacccgag caaattaacg
     6481 caaatcgcta ttgccgactc cgataaattg cgcgtcggtg attttgccgt agcggtcggt
     6541 aacccatttg gccttgggca aaccgccacc tctggcattg tttccgcatt aggccgcagc
     6601 gggttgaatc ttgaaggtct ggaaaacttt atccagacag atgcttccat taaccgcggt
     6661 aactccggcg gtgcactatt aaaccttaac ggtgagttaa ttggcatcaa cactgcaatc
     6721 cttgcgcctg gcggcgggag cgtcgggatt ggatttgcca tccccagtaa tatggcgcga
     6781 acactggcgc agcagcttat cgactttggt gaaatcaaac gcggtttgtt aggcatcaaa
     6841 ggcaccgaga tgagtgccga tatcgccaaa gccttcaacc ttgacgtgca gcgtggcgcg
     6901 tttgtcagcg aagtgttgcc aggttctggc tcggcaaaag cgggcgtcaa agcgggcgat
     6961 attattacca gcctcaacgg caaaccgctg aatagctttg ctgagttgcg ctctcgtatc
     7021 gcgaccaccg agccgggcac gaaagtgaag cttggcctgc tgcgtaacgg caaaccactg
     7081 gaagtagaag tgacgctcga taccagcacc tcttcgtcgg ccagcgctga aatgatcacg
     7141 ccagcgctgg aaggtgcaac gttgagcgat ggtcagctaa aagatggcgg caaaggtatt
     7201 aaaatcgatg aagttgtcaa aggaagccca gctgctcagg ctggcttgca aaaagacgat
     7261 gtgatcattg gcgtcaaccg cgatcgggtg aactcgattg ctgaaatgcg taaagtgctg
     7321 gcggcaaaac cggccatcat cgccctgcaa attgtacgcg gcaatgaaag catctatctg
     7381 ctgatgcgtt aatgtcgtaa accgggcatc aggcttacgt gtgatgtccg gttaactcgt
     7441 ggtatgctgc tgccgttccc ttttttaatg acgcctccat catgtttgtg aagctcttac
     7501 gttccgttgc gattggatta attgtcggcg ctattctgct ggttgccatg ccttcgctgc
     7561 gcagccttaa cccgctttcc actccgcaat ttgacagtac cgatgagacg cctgccagtt
     7621 ataatctggc ggttcgccgc gccgcgccag cggtggttaa cgtttacaac cgtggtttga
     7681 acaccaactc tcacaaccag cttgagatcc gcaccttggg atccggtgta atcatggatc
     7741 aacgcggtta tatcatcacc aataaacacg tcatcaacga cgccgatcag atcatcgtcg
     7801 ccttacagga tggtcgtgta tttgaagcat tgctggtggg atctgactct ctaaccgatc
     7861 tggcggtact taaaattaat gccactggcg gtttacctac cattccaatt aatgcacgtc
     7921 gcgtaccgca cattggcgac gtagtactgg cgatcggtaa cccgtacaac ctcgggcaga
     7981 ccattaccca ggggattatt agtgccacgg gtcgaatcgg tctgaacccg accgggcggc
     8041 aaaacttcct acaaaccgat gcttccatta accacggtaa ctctggcggc gcgctggtga
     8101 actcgctggg agaactgatg ggcattaaca cgctgtcgtt tgataagagt aacgatggcg
     8161 aaacgccgga aggtatcggc tttgcgattc ctttccagtt agcaaccaaa attatggata
     8221 agctgatccg cgatggtcgc gtgatccgcg gctacattgg tatcggcgga cgcgagatcg
     8281 caccactgca cgcgcagagc ggtggtatag atcaactgca agggatcgtg gttaatgaag
     8341 tgtcacctga cggcccagcg gcgaatgcgg gtattcaggt caacgatctg attatttcgg
     8401 tggataacaa accggccatc tctgctctgg agacgatgga tcaggtggcg gaaattcgcc
     8461 ctggttcggt gatcccggtt gtagtgatgc gtgatgataa gcagttaacg ctgcaggtca
     8521 ccattcagga atatccggca accaattaag tcgagtgctc aaaacaaaaa accggagtct
     8581 gtgctccggt tttttattat tcgctaatta attacttatt aacgaactct tcgcccaggg
     8641 cgatatcttt cttcagcgta tccagcatac cttccagtgc gctctgttca aatgcgctca
     8701 gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc ggttgagaga
     8761 agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg ccttgttcgc
     8821 cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc atagacaggg
     8881 ttgcagaccc accaccggct ttcgcttcaa ccacttcagt acctgcgttc tggatacgtt
     8941 tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt gacagcagcg
     9001 gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg cctggctgtt
     9061 tgcctttcag ttccgcaaca aaagtgttgg aacgaatgat atccagcgtg gtaacgccga
     9121 acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca atcgcaactg
     9181 tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcaggtt ttcgcaactt
     9241 gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa cgatccatac
     9301 ccggtttacg cgctacacct gcagagataa gaacgacatc tgcgccttcc agcgccggag
     9361 tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg ctcagatcga
     9421 cagccacacc gggagtcact ggagcgatat catacagaga gagttctgaa cctgaaggca
     9481 gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg ccgaggactg
     9541 cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc tccgctgccg
     9601 cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg tgtagtcacg
     9661 caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac gtaattaaca
     9721 gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct accgttcagc
     9781 caaaacaaca tcaatttgat aacaattaat ttacttttaa gcagaatttg catgccgtga
     9841 cgcaggcatg tttctcaata acgaaatttg ataaaatccc gctctttcat aacattattt
     9901 cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca ataatgttgt
     9961 atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga actagttaaa
    10021 gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat cgtcgccgcg
    10081 ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat gttgaccaag
    10141 tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct gccagctgaa
    10201 ctgggtgtac caaccacctc cagtccattg aagaatctgg tgctggatat cgactacaac
    10261 gatgccgttg tcgtgattca taccagccct ggcgcggcgc agttaattgc tcgcctgctg
    10321 gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga caccatcttt
    10381 accacccctg ctaacggttt caccgtcaaa gacctgtacg aagcgatttt agagctgttc
    10441 gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg ttgcttatcc
    10501 ctctcaaccc cctgctttcc cctgcgatta atttaacgaa tagtgcgttt tactgcgaca
    10561 tgtcattcac acaatgaata catacggtaa aaaaagcaca ttatgcaaaa ttcattatct
    10621 aattgaaaat actag
//
LOCUS       DAPGWD010000146        10396 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPGWD010000146 DAPGWD010000000
VERSION     DAPGWD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.147"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            5..580
                     /gene="ssuE"
                     /locus_tag="RHX88_003582"
     CDS             5..580
                     /gene="ssuE"
                     /locus_tag="RHX88_003582"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDV2232230.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            573..1532
                     /gene="ssuA"
                     /locus_tag="RHX88_003583"
     CDS             573..1532
                     /gene="ssuA"
                     /locus_tag="RHX88_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDV2232231.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEIAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1529..2674
                     /gene="ssuD"
                     /locus_tag="RHX88_003584"
     CDS             1529..2674
                     /gene="ssuD"
                     /locus_tag="RHX88_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDV2232232.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2685..3476
                     /gene="ssuC"
                     /locus_tag="RHX88_003585"
     CDS             2685..3476
                     /gene="ssuC"
                     /locus_tag="RHX88_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDV2232233.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3473..4240
                     /gene="ssuB"
                     /locus_tag="RHX88_003586"
     CDS             3473..4240
                     /gene="ssuB"
                     /locus_tag="RHX88_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV2232234.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4447..7059)
                     /gene="pepN"
                     /locus_tag="RHX88_003587"
     CDS             complement(4447..7059)
                     /gene="pepN"
                     /locus_tag="RHX88_003587"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDV2232235.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7325..8527
                     /gene="pncB"
                     /locus_tag="RHX88_003588"
     CDS             7325..8527
                     /gene="pncB"
                     /locus_tag="RHX88_003588"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDV2232236.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8696..10096
                     /gene="asnS"
                     /locus_tag="RHX88_003589"
     CDS             8696..10096
                     /gene="asnS"
                     /locus_tag="RHX88_003589"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDV2232237.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2338 a         2696 c         2799 g         2563 t
ORIGIN      
        1 gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct ccagctcctt
       61 gctggaatat gcgcgggaaa aactaaatgg cctcgatgta gaggtttatc actggaatct
      121 gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg cactcaagac
      181 cttcaccgaa cagctgcaac aggctgatgg actgatagtc gctacacctg tgtataaagc
      241 cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg ctttgcaagg
      301 caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg cggtcgatta
      361 tgcccttaaa ccagttttaa gcgcactgaa agcccaggag atcctgcacg gcgtgtttgc
      421 cgatgactca caagtcattg attaccatca cagaccccag ttcacgccaa atctgcaaac
      481 ccgtcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg atgttcaggt
      541 tcctgacctt ctgtctctgc gaggtaatgt ccatgcgtaa catcattaaa ctggcgctgg
      601 cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct gaagcgttac
      661 gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac cagttactgg
      721 aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt ccgcaaatgt
      781 tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt ccgccaatct
      841 ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg aagcccaaag
      901 ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat cttaaaggtc
      961 acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt gcactgcgtc
     1021 aggccggact taagtttacc gatatacaac ccacttacct gacacctgcc gatgcccgcg
     1081 ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac tactccgctg
     1141 cgttattaca gggcggcgtg cgggtgttga aagacggcac cgatctcaat caaaccggat
     1201 cgttttatct ggcagctcgc ccctatgcag aaaaaaacgg cgcttttatt cagggcgtac
     1261 tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgagcaa agcatcgctt
     1321 tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta gatcatcgcc
     1381 ctcctaccac catcaaaccg gttaacgccg agattgccgc cttacagcag caaacggcag
     1441 atctgtttta tgaaaatcgt ctggtgccga aaaaagtcga tattcgccag cgcatctggc
     1501 agcccactca actggaagga aaacaattat gagtctgaat atgttctggt ttttaccgac
     1561 ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg atcacggtta
     1621 tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc tgatcccaac
     1681 ggggcgatcg tgtgaagatg cgtggctggt ggcggcgtcg atgatcccgg tgacgcagcg
     1741 gctgaagttt cttgtcgccc tgcgccccag cgtaacctca cctaccgttg ccgctcgcca
     1801 ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg tcacaggcag
     1861 cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc gctacgaagc
     1921 ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg tcaatttcaa
     1981 cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc aacagcctta
     2041 tcctccgctt tactttggcg gatcgtcaga cgtcgcccag gagctggcgg cagaacaagt
     2101 tgatctctac ctcacctggg gcgaaccgcc agaactagta aaagagaaga tcgaacaagt
     2161 gcgggcgaaa gctgccgcgc atggacgcaa aattcgtttc ggtattcgtc tgcatgtgat
     2221 tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct cgcatcttga
     2281 tgatgaaact atcgccaaag cacaggccgc attcgcccgg acggattccg taggacaaca
     2341 gcgaatggcg gcgttacata acggcaagcg cgacaatctg gagatcagcc ccaatttatg
     2401 ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg atggtcctac
     2461 ggtcgctgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg tgctttcggg
     2521 ctatccgcat ctggaagaag cgtatcgggt cggcgagttg ctgttcccac atctggatgt
     2581 cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag cggtggcgaa
     2641 tgattttatc ccccgtaaag tcgcgcaaag ttaaggaaga aataatggca acgccagtga
     2701 agaagtggtt attgcgcgtt gccccctggt ttttaccggt gggcatcgtg gcggtgtggc
     2761 aactggcctc ctcagttggc tggctgtcga cgcgtatttt gccctcaccg gaaggggtgg
     2821 tgacggcgtt ctggacgctc tccgccagcg gcgaactgtg gcagcatctg gcgatcagtt
     2881 cctggcgggc gctgattggc ttttcaattg gcggatcgct ggggttgatt ttgggactga
     2941 ttagcgggct atctcgctgg ggagaacggc tgctggatac ctcaattcag atgttgcgca
     3001 acgtaccgca tctggcgctg atcccgctgg tgattttgtg gtttggcatt gatgaatccg
     3061 caaaaatctt tctggtggcg ctcggcacgc tattccccat ttatatcaac acctggcatg
     3121 gcatccgtaa tatcgatcgc ggtctggtgg aaatggcgcg tagctatgga ttatccggca
     3181 taccgctgtt tatccatgtg atcctgcctg gtgccctgcc ctcaattatg gtcggcgtgc
     3241 gttttgcgtt gggcctgatg tggctgacgc tgattgttgc cgaaaccatt tctgccaatt
     3301 caggcattgg ttatctggcg atgaatgcgc gggagttttt gcaaacggac gtggtggtag
     3361 tcgctattat tctttacgcc ctgctcggca aacttgctga cgtcagcgcg cagctgttag
     3421 agcgcctctg gttgcgctgg aacccggctt atcatttgaa ggaggccacg gtatgaatac
     3481 tgctcgtctg aaccagggca caccattgtt gctcaatgca gtaagcaaac attacgcgga
     3541 aaatatcgtc ctgaaccaac tggatttaca tattccggca ggtcagtttg tggcggtggt
     3601 gggccgcagc ggtggtggca aaagtaccct gctgcgcctg ctggcaggtc tggaaacgcc
     3661 aaccgcaggc gatgtgttag cgggcaccac accgctggct gagattcagg aagacacgcg
     3721 aatgatgttt caggatgcgc gtctgctgcc gtggaaatcg gtgattgata acgttgggtt
     3781 aggccttaaa ggccagtggc gcgatgccgc gcgtcgggcg ttggctgcag taggactgga
     3841 gaatcgcgcc ggggaatggc ctgccgcact ttctggcggg cagaaacagc gtgtggcgct
     3901 ggcaagagcg ttgattcatc gaccgggttt attgttgctt gatgaaccgc tcggggcgct
     3961 ggacgcctta acgcgactcg agatgcagga tttgattgtg tcactttggc agcagcacgg
     4021 ctttaccgta ctgttggtga cgcatgatgt gagcgaagcg gtagcaatgg ctgaccgggt
     4081 gctgttaatt gaagagggaa aaattggtct ggatttgacg gtggatatcc ctcgtccgcg
     4141 ccggttaggg tcggtgcgat tagcggagct ggaagcggaa gtattgcaac gggtaatgcg
     4201 gcgtgggcat tctgaacaac tgattcggcg tcatggttaa tatgccggat gtggcgcaca
     4261 aaaccagcat tttcaacgga ctagtaggcc tgataagaca cgtcaagcat cgcatcaggc
     4321 agttgggcac ggctgccgga tgcggcgtga acgccttatc cagcctacaa aaccagcaat
     4381 ttcaacggag ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca ttttggctca
     4441 aaagtatcaa gccagtgctt tggtgatctt ctcgtacaga tcgccagaga gattttccag
     4501 ccctttcaac tgttccagcg ccgcgcgcat tttctcctga cgtttggcat cataacgttt
     4561 caggcgaatc agcggttcaa tcagacgtga agctacctgc gggttacggc tgttgaggtc
     4621 ggtaagcatt tccaccagga actggtaacc gctgccatct tcggcatgga acgctgccgg
     4681 gttgctgccc gcaaacgcac caatcaacga acgaatacgg ttcgggttgc tcatggtaaa
     4741 tgagcgatgc tgcaacagac cacgcaccgt ctccagcaca ttcgccgccg ggctggtggc
     4801 ttgcaggata aaccatttat ccatcaccag accgtcctga tgccacttat cgtcgtactc
     4861 ctgcatcagc gcgtcacggc aaggcagctg tgcagcaacc gccgcagaaa gcgctgccag
     4921 cgcatcggtc atattgtttg cttcatggta ttgcttgctc accagcacgt cagccagatg
     4981 cgtttcacca aaagcaagga agcgcaggca ggcattacgc agagtgcgtt ttgcaatatc
     5041 ttcatgctca acacggtact cgctctggta attcgcgttg taaatagcca gcagctcatc
     5101 cgccagttca gtcgccagag tacgagtgag tgcttcgcgt acttcggcaa tagcaatcgg
     5161 gtcgatgata tcgaacagtt cagccatttc atttacagaa ggcagcgtca ggatttctgc
     5221 cgccagcgcc ggatcaatct tctcatcgag cagcaccgcg cggaaagcgt cagccacatg
     5281 taccggcaga gacagcggct gcccttgctg atgacgggcg acgttcagct tgatgtaggt
     5341 tgccagcaaa ctttgcgccg catcccaacg ggagaaatca ttacgcgcat gacgcatcag
     5401 gaaggtcagt tgctgatcgc tccacttata ttccagtttc actggcgcag agaattcgca
     5461 cagcagcgca ggcaccggct ggaagtagac attatcaaaa acaaaggtct gttccgcctg
     5521 ggtgacgttc agcacggaat tcaccggatg accgcctttc tgcaacggga tcactttgcc
     5581 ttcgttgtca tacaattcga tggcaaatgg aatatgcagc ggctgttttt ctgcctgatc
     5641 cggcgtggct ggcgtgcgct ggctgatggt cagggtgtac tgctcggttt ccggattgta
     5701 gtcgtctttg acggtcacaa tcggcgtgcc ggactggctg taccaacggc ggaaatggga
     5761 gagatcgaca ttcgacgcat cttccatcgc ctgcacaaag tcgtcgcagg ttgccgcgct
     5821 gccatcatgg cgctcgaaat agagttgcat ccctttctgg aagttttctt cgcccagcag
     5881 ggtgtggatc atgcgaatca cttccgcgcc cttctcgtaa acggtcaggg tgtagaagtt
     5941 gttcatctca atgaccatat ccgggcggat cgggtgcgcc atcgggctgg cgtcttccgc
     6001 aaactgcaat ccgcgcatgg tgcgcacatt attgatacgg tttaccgcgc gggaaccaag
     6061 gtcagagctg aattcctgat cgcggaagac ggttaaacct tctttcaggc tgagctggaa
     6121 ccagtcgcgg caggtcactc ggttaccggt ccagttatgg aaatattcat ggccgataac
     6181 gcgttcaata tcgagataat ctttgtcggt ggcggtgtcg gtgcgggcca acacatattt
     6241 ggagttaaag atattcagcc ccttattctc cattgcgccc atattgaaga aatccaccgc
     6301 gacgatcata tagatgtcga ggtcatactc caggccaaag cgttcttcat cccatttcat
     6361 ggagtttttc agcgaggtca tcgcccacgg cgcgcgatca aggttgccgc gatcgacgta
     6421 cagctccagt gctacttcgc gaccagaacg cgtggtaaag gtatcgcgca gtacatcaaa
     6481 gtcgcctgcc accagcgcaa acaggtagca cggtttcggg aacgggtcct gccactgtac
     6541 ccaatggcgt ccgttttcga gttcgccttg cgcaacgcgg ttaccattgg aaagcaggaa
     6601 gggatatttg attttatcgg caataatttt ggtggtaaaa cgcgccagca cgtccgggcg
     6661 gtcgagataa tacgtaatat ggcggaaacc ttcggcttca cactgggtgc aaagcgcatc
     6721 gcctgactga taaagccctt ccagcgccgt attcgccgcc gggcttattt cattaatgat
     6781 cttaagcgta aaacgctccg gcaaattact gatgaccagt gcgccctctt cttctttcca
     6841 ggcggtccac ggctcatcat taatatgaac agaaaccagt ttgaggtctt cgccgttgag
     6901 acgaagcgga gcatctgatg caccatgacg gacagcctgg ctgaccgcgg taacgaccgt
     6961 cttttgcgcg tcgaggtcaa aggtcaagtc aatatcagta atctggtaat ccggcgcacg
     7021 ataatcgtga cggtatttgg cttgtggctg ttgagtcata aataaccttt agcatctttt
     7081 atagagtctg gtgttcagtc tattcctgtt gcgtaaatcg cgctatgcag aatcttcatc
     7141 ttttcaggta caaacgcctt tattgctaca tttttataac atacaccgcg taatgccatc
     7201 gaccagaaag gtggcatatg gtgtgatcgg ggttcaataa attgcgaaac aaggtatact
     7261 ccagcagttc ctgaagatgt ttattgtact aaacgctcct gtacgaggac gctactgcgc
     7321 acctatgaca caattcgctt ctcctgttct gcactcgttg ctggatacag atgcttataa
     7381 gttgcatatg cagcaagccg tgtttcatca ctattacgat gtgcatgtcg cggcggagtt
     7441 tcgttgccga ggtgacgatc tgctgggtat ttatgccgat gctattcgtg aacagattca
     7501 ggcgatgcag cacctgcgcc tgcaggatga tgaatatcag tggctctctg ctctgccttt
     7561 ctttaaggcc gactatctta actggttacg cgagttccgc tttaacccgg aacaagtcac
     7621 cgtatccaac gataatggca agctggatat tcgtttaagc ggcccgtggc gtgaagtcat
     7681 cctctgggaa gttcctttgc tggcggttat cagtgaaatg gtacatcgct atcgctcacc
     7741 gcaggccgac gttgcgcaag ccctcgacac gctggaaagc aaattagtcg acttctcggc
     7801 gttaaccgcc ggccttgata tgtcgcgctt ccatcttatg gattttggca cccgtcgccg
     7861 tttttctcgc gaagtacaag aaaccatcgt taagcgtctg caacaggaat cctggttcgt
     7921 gggcaccagc aactacgatc tggcgcgtcg gctttccctc acgccgatgg gaacacaggc
     7981 acacgaatgg ttccaggcac atcagcaaat cagcccggat ctagccaaca gccagcgagc
     8041 tgcacttgct gcctggctgg aagaatatcc cgaccaactt ggcattgcat taaccgactg
     8101 catcactatg gatgctttcc tgcgtgattt cggtgtcgag ttcgccagtc gctatcaggg
     8161 cctgcgtcat gactctggcg acccggttga atggggtgaa aaagccattg cacattatga
     8221 aaagctggga attgatccac agagtaaaac tctggttttc tctgacaatc tggatttacg
     8281 caaagcggtt gagctatacc gccacttctc ttcccgcgtg caattaagtt ttggtattgg
     8341 gactcgcctg acctgcgata tcccccaggt aaaacccctg aatattgtga ttaagttggt
     8401 tgagtgtaac ggtaaaccgg tggcgaaact ttctgacagc cctggcaaaa ctatctgcca
     8461 cgataaagcg tttgttcggg cgctgcgcaa agcgttcgac cttccgcata ttaaaaaagc
     8521 cagttaatat catcagggag ctaatcggct cccttttttt acctttaatt ccgaaatctt
     8581 tcgctgcatt tgcgaattct gcttgtctga ttgcagatgc caggtaacat aggtatcccc
     8641 ccatttacgg gtggacaagt gtttattttt tccgactatt aacagagaga atattatgag
     8701 cgttgtgcct gtagccgacg tactccaggg ccgcgtagcc gttgacagcg aagtcaccgt
     8761 acgcggatgg gtacgtaccc gccgagattc aaaagctggc atctccttcc tcgccgttta
     8821 tgacggttcc tgctttgatc ctgtacaggc tgtcatcaat aattctctgc ccaattacaa
     8881 tgaagacgtc ctgcgtctga ccaccggctg ctcggtcatt gtgacgggta aagtcgtggc
     8941 gtcgccgggc caggggcaac aatttgaaat tcaggccagc aaagttgaag ttgctggttg
     9001 ggttgaagat ccagacactt acccgatggc ggcaaaacgc cacagcattg agtatctgcg
     9061 tgaagtcgct cacctgcgtc cgcgcacaaa cctgattggt gccgtcgcgc gcgttcgcca
     9121 tacgctggcg caggcgctgc atcgcttctt taacgagcag ggattcttct gggtttcaac
     9181 gccactgatt accgcatctg ataccgaagg tgcaggcgaa atgttccgcg tttctacgct
     9241 ggatctggaa aacctgccgc gtaacgatca gggcaaagtg gatttcgaca aagacttctt
     9301 tggtaaagag tctttcctga ccgtatctgg ccagttgaac ggcgaaacct acgcttgcgc
     9361 attatccaaa atttatacct tcggcccgac tttccgtgct gaaaactcca acaccagccg
     9421 tcacctggcg gaattctgga tgctggagcc ggaagtggcg tttgctaacc tgaacgatat
     9481 cgcgggtctg gctgaagcca tgctgaaata tgtcttcaaa gcggttctcg aagaacgcgc
     9541 agacgacatg aaattcttcg ctgaacgcgt agataaagat gccgtttcac gtctggaacg
     9601 cttcattgag gccgatttcg cgcaggtgga ttacaccgac gcagtgacca ttctcgaaaa
     9661 ctgcggcagg aagtttgaaa acccggttta ctggggcgtc gatctctctt ctgagcatga
     9721 gcgttatctg gcggaagaac actttaaagc accggtagtg gttaaaaact atccgaaaga
     9781 tattaaagcg ttctatatgc gcctcaacga agacggtaaa accgttgcgg ctatggacgt
     9841 tctggctccg ggcatcggtg agatcattgg tggctcccag cgtgaagaac gtctggacgt
     9901 tctggacgag cgtatgctgg aaatgggcct gaataaagaa gattactggt ggtatcgcga
     9961 tctgcgtcgc tacggtactg ttccgcattc aggtttcggt cttggttttg aacgcctgat
    10021 tgcttacgta actggcgtgc aaaacgtgcg tgatgtgatt ccgttcccac gtactccgcg
    10081 caacgccagc ttctgatttt cttcctcaaa gagccagcgc ctgctggctc ttttttatcc
    10141 tttctgtcaa gttatctgtt tgttaagtca agcaatctat ttgcaacccc gccataacga
    10201 tcatcctgtt acggaatatt acattgcaac atttacgcac aaaaaataac ccgcattctt
    10261 attgcggatt agttttttct tagctgatag cacaattttc atactatttt ttggcattct
    10321 ggatgtctga aagaggattt tgtgccaggt cgataaagtt tccatcagaa acaaaatttc
    10381 cgtttagtta atttaa
//
LOCUS       DAPGWD010000147        10333 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPGWD010000147 DAPGWD010000000
VERSION     DAPGWD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.148"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="RHX88_003590"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="RHX88_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDV2232238.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="pgeF"
                     /locus_tag="RHX88_003591"
     CDS             complement(2915..3646)
                     /gene="pgeF"
                     /locus_tag="RHX88_003591"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDV2232239.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="RHX88_003592"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="RHX88_003592"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDV2232240.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="RHX88_003593"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="RHX88_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDV2232241.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="RHX88_003594"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="RHX88_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDV2232242.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="RHX88_003595"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="RHX88_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDV2232243.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="RHX88_003596"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="RHX88_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDV2232244.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="RHX88_003597"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="RHX88_003597"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDV2232245.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRMMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVID
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="RHX88_003598"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="RHX88_003598"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDV2232246.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9972..>10333
                     /locus_tag="RHX88_003599"
     CDS             9972..>10333
                     /locus_tag="RHX88_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDV2232247.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRL"
BASE COUNT         2645 a         2732 c         2426 g         2530 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa aacatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accaacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat tcccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcaatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aacccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 caggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccttgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 tgaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctccg cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atgttttcag gtttatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccag
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagtga gtcatacggg ggcaagctca cgccgccgat acgagtggag ctacaggccg
     3601 caacaccttt tggcagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcgtggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatt ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaattg ccaccgcttc atactcacga gtaatttcac gcctttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagcg ttgatggcaa cctgctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt gattttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgtgat attgatcgcg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccaacaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcactact gatttatcca
     6961 ccatcaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatccaca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcaa atcagcgaca aaacttcacc cgatttgtgg
     7201 tgttggcgcg taaagccatt aacgtgtctg atcaggttcc ggcgaaaacc acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtactg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 tgggggaaat cacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgcga atcatccggc actggattat
     7561 tactggcgat tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aatgatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc caggtgcagc ccgtaagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgtcgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcat ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgta agctgcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcggaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggttcta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacactcc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ctttgaccag caccgactgg tatgatgttg
    10081 gcatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccga ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 caatacgatt aaa
//
LOCUS       DAPGWD010000148        10138 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPGWD010000148 DAPGWD010000000
VERSION     DAPGWD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.149"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(78..854)
                     /gene="yaaA"
                     /locus_tag="RHX88_003600"
     CDS             complement(78..854)
                     /gene="yaaA"
                     /locus_tag="RHX88_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDV2232248.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(924..2354)
                     /locus_tag="RHX88_003601"
     CDS             complement(924..2354)
                     /locus_tag="RHX88_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDV2232249.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFTLLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2633..3586
                     /gene="tal"
                     /locus_tag="RHX88_003602"
     CDS             2633..3586
                     /gene="tal"
                     /locus_tag="RHX88_003602"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV2232250.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANSDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3701..4288
                     /gene="mog"
                     /locus_tag="RHX88_003603"
     CDS             3701..4288
                     /gene="mog"
                     /locus_tag="RHX88_003603"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDV2232251.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4323..4889)
                     /gene="satP"
                     /locus_tag="RHX88_003604"
     CDS             complement(4323..4889)
                     /gene="satP"
                     /locus_tag="RHX88_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDV2232252.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5038..5751)
                     /gene="msyB"
                     /locus_tag="RHX88_003605"
     CDS             complement(5038..5751)
                     /gene="msyB"
                     /locus_tag="RHX88_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV2232253.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5777..6181)
                     /locus_tag="RHX88_003606"
     CDS             complement(5777..6181)
                     /locus_tag="RHX88_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDV2232254.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6558..8474
                     /gene="dnaK"
                     /locus_tag="RHX88_003607"
     CDS             6558..8474
                     /gene="dnaK"
                     /locus_tag="RHX88_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDV2232255.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8563..9693
                     /gene="dnaJ"
                     /locus_tag="RHX88_003608"
     CDS             8563..9693
                     /gene="dnaJ"
                     /locus_tag="RHX88_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDV2232256.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9797..9949)
                     /gene="mokC"
                     /locus_tag="RHX88_003609"
     CDS             complement(9797..9949)
                     /gene="mokC"
                     /locus_tag="RHX88_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDV2232257.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2662 a         2543 c         2629 g         2304 t
ORIGIN      
        1 ttctgcaata tattgaatct gcatgctttt gtaggccgga taaggcgttc acgccgcatc
       61 cggcattgac tacaaactta acgctgctcg tagcgtttaa acaccagttc gccattgctg
      121 gaggaagctt catcaaagaa gtaaccttcg ctattaaaac cagtcagttg ctctggtttg
      181 gtcagccgat tttcaataat aaaacgactc atcagaccgc gtgctttctt agcgtagaag
      241 ctgattatct taaatttgcc gttcttctca tcgaggaaca ccggcttgat aatctcggca
      301 ttcaatttct tcggcttcac cgatttaaaa tactcatctg acgccagatt aatcaccaca
      361 ttatcgcctt gtgctgcgag cgcctcgttc agcttgttgg tgatgatatc tccccagaat
      421 tgatacagat ctttccctcg ggcattctca agacggatcc ccatttccag acgataaggc
      481 tgcattaaat cgagcgggcg cagtacgcca tacaagccgg aaagcattcg caaatgctgt
      541 tgggcaaaat cgaaatcgtc ttcgctgaag gtttcggcct gcaagccggt gtagacatca
      601 cctttaaacg ccagaatcgc ctggcgggca ttctccggcg tgaaatctgg ctgccagtca
      661 tgaaagcgag cggcgttgat acccgccagt ttgtcgctga tgcgcatcag cgtgctaatc
      721 tgcggaggcg tcagtttccg cgcttcatgg atcaactgct gggaattgtc taacagctcc
      781 ggcagcgtat agcgcatggt ggtcaacggg ctttggtaat caagcgtttt cgcaggtgaa
      841 ataagaatca gcatatccag tccttgcagg aaatttatgc cgactttagc aaaaaaagag
      901 aatgagttga tcgatagttg tgattactcc tgcgaaacat catcccacgc gtccggagaa
      961 agctggcgac cgatatccgg ataacgcaac ggatcaaaca ccgggcgcac gccgagttta
     1021 cgctggcgta gataatcact ggcaatggta tgaaccacag gcgagagcag taaaatggcg
     1081 gtcaaattgg taatagccat gcaggccatt atgatatctg ccagttgcca catcagcgga
     1141 agacttagca aggtgccgcc gatgaccgtt gcgaaggtgc agatccgcaa acaccagatc
     1201 gctttagggt tgttcaggcg taaaaagaag agattgtttt cggcataaat gtagttggca
     1261 acgatggagc tgaaggcaaa cagaataacc acgagggtaa caaactcagc accccaggaa
     1321 cccattagca cccgcatcgc cttctggata agctgaatac cttccagcgg catgtaggtt
     1381 gtgccgttac ccgccagtaa tatcagcatg gcgcttgccg tacagatgac cagggtgtcg
     1441 ataaaaatgc caatcatctg gacaatccct tgagctgccg gatgcggagg ccaggacgca
     1501 gctgccgctg ctgcgtttgg tgtagaaccc attcccgcct cattggaaaa catactgcgc
     1561 tgaaaaccgt tagtaatcgc ctggcttaag gtatatcccg ccgcgccgcc tgccgcttcc
     1621 tgccagccaa aagcactctc aaaaatagac caaatgacgt ggggaagttg cccgatattc
     1681 attacgcaaa tcaccaggct ggtcagtacc cagattatcg ccatcaacgg gacaaaaccc
     1741 tgcatgagcc gggcgacgcc atgaagaccg cgagtgattg ccagcagagt aaagacagcg
     1801 agaataatgc ctgtcaccag cgggggaaaa tcaaaagaaa aactcagggc tcgggcaacg
     1861 gcgttcgcct gaactccgct gaaaattatg ccataggcga tgagcaaaaa gacggcgaac
     1921 agaacgccca tccaacgcat ccccagcccg cgcgccatat accatgccgg tccgccacga
     1981 aactgtccat tgacgtcacg ttctttataa agttgtgcca gagaacattc ggcaaacgag
     2041 gtcgccatgc cgataaacgc ggcaacccac atccaaaaga cggctccagg tccaccggcg
     2101 gtaattgcca gcgcaacgcc ggccaggttg ccgctaccca cgcgcgccgc aagactggta
     2161 cacaatgact gaaatgaggt taaaccgcct ggctgtggat gaatgctatt tttaagactt
     2221 ttgccaaact ggcggatgta gcgaaactgc acaaatccgg tgcgaaaagt gaaccaacaa
     2281 cctgcgccga agagcaggta aatcattacc gatccccaaa ggacgctgtt aataaaggag
     2341 aaaaaatctg gcatgcatat ccctcttatt gccggtcgcg atgactttcc tgtgtaaacg
     2401 ttaccaattg tttaagaagt atatacgcta cgaggtactt gataacttct gcgtagcata
     2461 catgaggttt tgtataaaaa tggcgggcga tatcaacgca gtgtcagaaa tccgaaacag
     2521 tctcgcctgg cgataaccgt cttgtcggcg gttgcgctga cgttgcgtcg tgatatcatc
     2581 agggcagacc ggttacatcc ccctaacaag ctgtttaaag agaaatacta tcatgacgga
     2641 caaattgacc tcccttcgtc agtacaccac cgtagtggcc gacactgggg acatcgcggc
     2701 aatgaagctg tatcaaccgc aggatgccac aaccaaccct tctctcattc ttaacgcagc
     2761 gcagattccg gaataccgta agttgattga tgatgctgtc gcttgggcga aacagcagag
     2821 caacgatcgc gcgcagcaga tcgtggacgc gaccgacaaa ctggcagtaa atattggtct
     2881 ggaaatcctg aaactggttc cgggccgtat ctcaactgaa gttgatgcgc gtctttccta
     2941 tgacaccgaa gcgtcaattg cgaaagcaaa acgcctgatc aaactctaca acgatgctgg
     3001 tattagcaac gatcgtattc tgatcaaact ggcttctacc tggcagggta tccgtgctgc
     3061 agaacagctg gaaaaagaag gcatcaactg taacctgacc ctgctgttct ccttcgctca
     3121 ggctcgtgct tgtgcggaag caggcgtgtt cctgatctcg ccatttgttg gccgtattct
     3181 tgactggtac aaagcgaata gtgataagaa agagtacgct ccggcagaag atccgggcgt
     3241 ggtttctgta tctgaaatct accagtacta caaagagcac ggttatgaaa cagtggttat
     3301 gggcgcaagc ttccgtaaca tcggcgaaat tctggaactg gcaggctgcg accgtctgac
     3361 catcgcaccg gcactgctga aagagctggc ggagagcgaa ggggctatcg aacgtaaact
     3421 gtcttacacc ggcgaagtga aagcgcgtcc ggcgcgtatc actgagtccg agttcctgtg
     3481 gcagcacaac caggatccaa tggcagtaga taaactggcg gaaggtatcc gtaagtttgc
     3541 tattgaccag gaaaaactgg aaaaaatgat cggcgatctg ctgtaatcat tcttagcgtg
     3601 accgggaagt cggtcacgct acctcttctg aagcctgtct gtcactccct tcgcagtgta
     3661 tcattctgtt taacgagact gtttaaacgg aaaaatcttg atgaatactt tacgtattgg
     3721 cttagtttcc atctctgatc gcgcatccag cggcgtttat caggataaag gcatccctgc
     3781 gctggaagaa tggctgacat cggcgctaac cacgccgttt gaactggaaa cccgcttaat
     3841 ccccgatgag caggcgatca tcgaacaaac gttgtgtgag ctagtggatg aaatgagttg
     3901 ccatctggtg ctcaccacgg gcggaactgg cccggcgcgt cgtgacgtaa cgcccgatgc
     3961 gacgctggca gtagcggacc gcgagatgcc tggctttggt gaacagatgc gccagatcag
     4021 cctgcatttt gtaccaactg cgatcctttc gcgtcaggtg ggcgtgattc gcaaacaggc
     4081 gctgatcctt aacttacccg gtcagccgaa gtctattaaa gagacgctgg aaggtgtgaa
     4141 ggacgctgcg ggtaacgttg tggtacacgg tatttttgcc agcgtaccgt actgcattca
     4201 gttgctggaa gggccatacg ttgaaacggc accggaagtg gttgcagcat tcagaccgaa
     4261 gagtgcaaga cgcgacgtta gcgaataaaa aaatcccccc gagcgggggg agctcaaaac
     4321 aattagtggg attcaccaat cggcagaacg gtgcgaccaa actgctcgtt cagtacttca
     4381 cccatcgcca gatagattgc gctggcaccg cagatcagcc caatccagcc ggcaaagtgg
     4441 atgattgcgg cgttaccggc aatgttaccg atcgccagca gggcaaacag cacggtcagg
     4501 ctaaagaaaa cgaattgcag aacgcgtgcg cctttcagcg tgccgaagaa cataaacagc
     4561 gtaaatacgc cccacagacc caggtagaca ccaaggaact gtgcatttgg cgcatcggtc
     4621 agacccagtt tcggcatcag cagaatcgca accagcgtca gccagaaaga accgtaagag
     4681 gtgaatgcgg ttaaaccgaa agtgttgcct tttttgtact ccagcagacc agcaaaaatt
     4741 tgcgcgatgc cgccgtagaa aatgcccatg gcaagaataa taccgtccag agcgaaataa
     4801 cccacgttgt gcaggttaag cagaatggtg gtcatgccga agcccatcag gcccagcggt
     4861 gccggattag ccaacttagt gttgcccata attcctcaaa aatcatcatc gaatgaatgg
     4921 tgaaataatt tccctgaata actgtagtgt tttcagggcg cggcataata atcagccagt
     4981 ggggcagtgt ctacgatctt ttgaggggaa aatgaaaatt ttccccggtt tccggtatca
     5041 gacctgagtg gcgctaacca tccggcgcag gcaggcgatt tgcagtacgg ctggaatcgt
     5101 cacgcgatag gcgctgccgc tgaccgcttt aaccccattt agtgccgcac ctacagggcc
     5161 tcccagcccc gcgccgcgca gcaaaccatg cccaagtacg ctcattgctg cgtgggtgcg
     5221 taaaatgcgg gtcagttggc tggaaagcaa atgcgacacg ccttttgcca ataatttgtc
     5281 tttcatcagc agcggcagca gctcttccag ctcattcacc ctggcatcga tcgcgtgcag
     5341 aaactcctgc ttatgttcct cgtccatatt cttccaggtg ttacgcagaa attgttccag
     5401 taactgttgc tcaatctcaa acgtagacat ctctttgtcg gctttcagct tcaatcgttt
     5461 tgaaacatcg agcaaaatgg cccgatacaa tttaccgtgt ccgcgcagtt tgttggcgat
     5521 actatcgcca ccaaaatgct gtaattctcc agcaatcagc tgccagttgc ggcgatgttg
     5581 ctcgggatgc ccttccatcg atttaaacag ttcgttgcgc atcagtacgc tggagaggcg
     5641 agttttgcct ttttcattat gggtgagcaa tcgggcgaaa tttgccaact gttcctcact
     5701 gcaatgctgg agaaaatcca gatctgaatc attcaggtaa ttaacattca ttttttgtgg
     5761 cttctatatt ctggcgttag tcgtcgccga taattttcag cgtggccata tccgatgagt
     5821 tcaccgtatg accggaaaag gtgatttttg agacgcagcg tttattgtcg ttatcactgt
     5881 taatgttgat ccagtcagtg gtttgccctt cttttatttc tgaaggaata ttcagactct
     5941 gactggcgct acgggcggct ttgaaataaa ccgatgcacc gcttaactgt aaatcgccat
     6001 ggtcggcaga gagttgtatg cgtttcacaa tgcgacaaac aggaagtttc agcgccagat
     6061 cgttggtttc gttacgcggc attgcaatga cgccgaggag tttatggtcg tttgcctgcg
     6121 ccgtgcagca cagcatcagg ctaatcgcca ggctggcgga aatcgtaaaa acggatttca
     6181 taaggattct cttagtggga agaggtagag ggatgaatac ccactagttt actgctgata
     6241 aagagaagat tcaggtacgt aatcttttct ttttattaca attttttgat gaataccttg
     6301 gctgcgattc attctttata tgaataaaat tgctgtcaat tttacgtctt gtcctgccat
     6361 atcgcgaaat ttctgcgcaa aagcacaaaa aatttttgca tctccccctt gatgacgtgg
     6421 gttacgaccc catttagtag tcaaccgcag tgagtgagtc tgcaaaaaaa tggaattggg
     6481 cagttgaaac cagacgtttc gcccctatta cagactcaca accacatgat gaccgaatat
     6541 atagtggaga cgtttagatg ggtaaaataa ttggtatcga cctgggtact accaactctt
     6601 gtgtagcgat tatggatggc accactcctc gcgtgctgga gaacgccgaa ggcgatcgca
     6661 ccacgccttc tatcattgcc tatacccagg atggtgaaac tctggttggt cagccggcta
     6721 aacgtcaggc agtgacgaac ccgcaaaaca ccctgtttgc gattaaacgc ctgattggcc
     6781 gccgcttcca ggacgaagaa gtacagcgtg atgtttccat catgccgttc aaaattattg
     6841 ctgctgataa cggcgacgca tgggtcgaag ttaaaggcca gaaaatggca ccgccgcaga
     6901 tctctgctga agtgctgaaa aaaatgaaga aaaccgctga agattacttg ggtgaaccgg
     6961 taactgaagc tgttatcacc gtaccggcat actttaacga tgctcagcgt caggcaacca
     7021 aagacgcagg ccgtatcgct ggtctggaag taaaacgtat catcaacgaa ccgaccgcag
     7081 ctgcgctggc ttacggtctg gacaaaggta ctggcaaccg tactatcgcg gtttatgacc
     7141 tgggtggtgg tactttcgat atttctatta tcgaaatcga cgaagttgac ggcgaaaaaa
     7201 ccttcgaagt tctggctacc aacggtgata cccacctggg tggtgaagac ttcgacagcc
     7261 gtctgatcaa ctacctggtt gaagaattca agaaagatca gggcattgac ctgcgcaacg
     7321 atccgctggc aatgcagcgc ctgaaagaag cggcagaaaa agcgaaaatc gaactgtctt
     7381 ccgctcagca gaccgacgtt aacctgccgt acatcactgc agatgcgacc ggtccgaaac
     7441 acatgaacat caaagtgact cgtgcgaaac tggaaagcct ggttgaagat ctggtaaacc
     7501 gttccattga gccgctgaaa gttgcactgc aggacgctgg cctgtccgta tctgatatcg
     7561 acgacgttat cctcgttggt ggtcagactc gtatgccaat ggttcagaag aaagttgctg
     7621 agttctttgg taaagagccg cgtaaagacg ttaacccgga cgaagctgta gcaatcggtg
     7681 ctgctgttca gggtggtgtt ctgactggtg acgtgaaaga cgtactgctg ctggacgtta
     7741 ccccgctgtc tctgggtatc gaaaccatgg gcggtgtgat gacgacgctg atcgcgaaaa
     7801 acaccactat cccgaccaag cacagccagg tgttctctac cgctgaagac aaccagtctg
     7861 cggtaaccat ccatgtgctg cagggtgaac gtaaacgtgc ggctgataac aaatctctgg
     7921 gtcagttcaa cctggatggt atcaacccgg caccgcgcgg catgccgcag atcgaagtta
     7981 ccttcgatat cgatgctgac ggtatcctgc acgtttccgc gaaagataaa aacagcggta
     8041 aagagcagaa gatcaccatc aaggcttctt ctggtctgaa cgaagatgaa atccagaaaa
     8101 tggtacgcga cgcagaagct aacgccgaag ctgaccgtaa gtttgaagag ctggtacaga
     8161 ctcgcaacca gggcgaccat ctgctgcaca gcacccgtaa gcaggttgaa gaagcaggcg
     8221 acaaactgcc ggctgacgac aaaactgcta tcgagtctgc actgactgca ctggaaactg
     8281 ctctgaaagg tgaagacaaa gccgctatcg aagcgaaaat gcaggagctg gcacaggttt
     8341 cccagaaact gatggaaatc gcccagcagc aacatgccca gcagcagact gccggtgctg
     8401 atgcttctgc aaacaacgcg aaagatgacg atgttgtcga cgctgaattt gaagaagtca
     8461 aagacaaaaa ataatcgccc tataaacggg taattatact gacacgggcg aaggggaatt
     8521 tcctctccgc ccgtgcattc atctaggggc aatttaaaaa agatggctaa gcaagattat
     8581 tacgagattt taggcgtttc caaaacagcg gaagagcgtg aaatcaaaaa ggcctacaaa
     8641 cgcctggcca tgaaatacca cccggaccgt aaccagggtg acaaagaggc cgaggcgaaa
     8701 tttaaagaga tcaaggaagc ttatgaagtt ctgaccgact cgcaaaaacg tgcggcatac
     8761 gatcagtatg gtcatgctgc gtttgagcaa ggtggtatgg gcggcggcgg ttttggcggc
     8821 ggcgcagact tcagcgatat ttttggtgac gttttcggcg atatttttgg cggcggacgt
     8881 ggtcgtcaac gtgcggcgcg cggtgctgat ttacgctata acatggagct caccctcgaa
     8941 gaagctgtac gtggcgtgac caaagagatc cgcattccga ctctggaaga gtgtgacgtt
     9001 tgccacggta gcggtgcaaa accaggtaca cagccgcaga cctgtccgac ctgtcatggt
     9061 tctggtcagg tgcagatgcg tcagggtttc tttgccgtgc agcagacctg tccacactgt
     9121 cagggtcgcg gtacgctgat caaagatccg tgcaacaaat gtcatggtca tggtcgtgtt
     9181 gagcgcagca aaacgctgtc cgttaaaatc ccggcagggg tggacactgg agaccgcatc
     9241 cgtcttgcgg gcgaaggtga agcgggcgag catggcgcac cggcaggcga tctgtacgtt
     9301 caggttcagg ttaaacagca cccgattttc gagcgtgaag gcaacaacct gtattgcgaa
     9361 gtcccgatca acttcgctat ggcggcgctg ggtggcgaaa tcgaagtacc gacccttgat
     9421 ggtcgcgtca aactgaaagt gcctggcgaa acccagactg gtaagctgtt ccgtatgcgc
     9481 ggtaaaggcg tcaagtctgt ccgcggtggc gcacagggtg atttactgtg ccgcgttgtc
     9541 gtcgaaacac cggtaggcct gaacgagaag cagaaacagc tgctgcaaga gctgcaagaa
     9601 agcttcggtg gcccaaccgg cgagcacaac agcccgcgct caaagagctt ctttgatggt
     9661 gtgaagaagt tttttgacga cctgacccgc taacctcccc aaaagcctgc ccgtgggcag
     9721 gcctgggtaa aaatagggtg cgttgaagat atgcgagcac ctgtaaagtg gcggggatca
     9781 ctccccgccg ttgctcttac tcggattcgt aagccgtgaa aacagcaacc tccgtctggc
     9841 cagttcggat gtgaacctca cagaggtctt ttctcgttac cagcgccgcc actacggcgg
     9901 tgatacagat gacgatcagg gcgacaatca tcgccttatg ctgcttcatt gctctcttct
     9961 ccttgacctt acggtcagta agaggcactc tacatgtgtt cagcatatag ggggcctcgg
    10021 gttgatggta aaatatcact cggggctttt ctctatctgc cgttcagcta atgcctgaga
    10081 cagacagcct caagcacccg ccgctattat atcgctctct ttaacccatt ctgtttta
//
LOCUS       DAPGWD010000149        10064 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPGWD010000149 DAPGWD010000000
VERSION     DAPGWD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.150"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            298..1449
                     /locus_tag="RHX88_003610"
     CDS             298..1449
                     /locus_tag="RHX88_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232258.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            1533..2404
                     /locus_tag="RHX88_003611"
                     /pseudo
     CDS             1533..2404
                     /locus_tag="RHX88_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            2622..5018
                     /locus_tag="RHX88_003612"
     CDS             2622..5018
                     /locus_tag="RHX88_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDV2232259.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     EALELMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTS
                     DLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCA
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            5015..5920
                     /locus_tag="RHX88_003613"
     CDS             5015..5920
                     /locus_tag="RHX88_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDV2232260.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITQVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQSETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            5917..6987
                     /locus_tag="RHX88_003614"
     CDS             5917..6987
                     /locus_tag="RHX88_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDV2232261.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAIARIK"
     gene            7123..7806
                     /locus_tag="RHX88_003615"
     CDS             7123..7806
                     /locus_tag="RHX88_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232262.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            7822..8232
                     /locus_tag="RHX88_003616"
     CDS             7822..8232
                     /locus_tag="RHX88_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232263.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            8453..9274
                     /locus_tag="RHX88_003617"
     CDS             8453..9274
                     /locus_tag="RHX88_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV2232264.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIF
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLEEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            9356..9835
                     /locus_tag="RHX88_003618"
     CDS             9356..9835
                     /locus_tag="RHX88_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2232265.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            9851..>10064
                     /locus_tag="RHX88_003619"
     CDS             9851..>10064
                     /locus_tag="RHX88_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232266.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLI"
BASE COUNT         2598 a         2502 c         2690 g         2274 t
ORIGIN      
        1 aatgagaagt ttacgggatg tgtatccggc acactatgac cggagaatat cagtcaagct
       61 taaaaagaca ataaatacag tgagttaaat acacagagaa aacgtagtat cagagggtac
      121 atcttacaac agagactgaa tttccggttt ttcataaccg gcgcattccg gaaagtgcag
      181 gcggcccgga ggttttctga cgccactacc tggtagattg ttctgtacat catgtacgga
      241 ggtttttgtt tgctttttta aaatctgcaa atgtgagaaa agcaggtgag gtgtattgtg
      301 ggaaaggaac tggatgatat ctgcaccagc tgtccgtata tcgatgctgt aaaacggcac
      361 aaacaacagc tcggtgccat agaggaatat acacagtggt taaaaaaaga accacgggca
      421 tcttattttt ttctgttcca gctgtatacc cgcattcaca atacattttt ctcccaaaag
      481 caacaactcc cgttcactcc cggtggcact cactgcccgg aacctgatgt cacccttcgg
      541 gacttaacgc tttcccctgg ctaccacagt gattatgccc ctcaacccat tccggagatg
      601 gacagttctg ctgttgttcc accaactaat gaaaacacct cgcctccgga agatactccc
      661 gacaacactc cggccggagg caataccgga caagcggaaa agaccagaaa cgcaggactc
      721 acacccattc ctgaaaaacg ttcaggaatg cccccggaac atctgcggtt tgccacgggg
      781 tttccatcgc aaccgaagat tgccggtccc agagaaaagc cgatgcgcac cgtgcatccg
      841 gataaaatct accgtgaaat tatatggttc tgctccagtt acctgctgaa atccggacca
      901 gaggctacga ggacgattat caactcagta ttctctgaat gggccagcat aaacaatgat
      961 tacccctccc ccttttcgtg ggtggacagc agggacagtg aacagtgtga ctggttatgg
     1021 aacgccatgc aggtccggtg tgtgggaacc ccgctgaatc cccttacccc ggagcagaaa
     1081 tactggtttg cctgcgccac gtttgataac tgggagggct ggaatgagca acaggtacag
     1141 tttttactgg aaagtaatcc cagacggaac agagcgaagt ttacgcaggt ctccttccag
     1201 gcccccagga ttcagcataa agcgattctt cttgatgagc tgaaaagtgc ccgggagcaa
     1261 caaaaaaggc gtgatgaacg tgctgatggt tccgtccctc tgaaactgtc cggaaaaatc
     1321 cacaaacagc ttgaaagtat tgcccgaagt cgtggtgtcc tcccaaaaaa actgctgaat
     1381 gaaatgattg agcaggcgta ccatgatttg gtggcaacca ggcagaacag ccaaatcgat
     1441 tccagataat ttcagacaga tattatttcc gtgaatcccc cgccaccttt ccggtgcgcg
     1501 gggttttgtc ttttttcacc gggaatacat gtatgaatcc gtctgatgcc attgaggcaa
     1561 ttgaaaaacc gctctcctcc ctgccttact cactttcccg tcacatcctg gaacatctgc
     1621 gcaaactcac cagtcacgaa cccgtgcctg gcattatggg taaaagcggg cccgtaaatc
     1681 ctcactctgt aatgcgctgt ttcaggggga ggtcaccccg gtcagtgatg ttcacgccgg
     1741 cacccgggaa gtgcagcgct tccgtctgaa tggccatggt cacagcatgg ttatcactga
     1801 cctgcccggg gtgggcgaga gccgggacag ggatgcagag tatgaagccc tgtaccgtga
     1861 cattctgctc gaactggacc tggtactgtg gctgattaaa gccgatgacc gtgccctgtc
     1921 tgtggatgag tatttctggc gacacatcct gcaccgggga catcagcggg tactgtttgt
     1981 ggtgatgcag gccgataaaa cggagccctg ccatgaatgg gatatggccg gtattcagcc
     2041 ttctcccgca gaggcacaga acattcgcga aaaaacggag gcggtattcc gtctgttccg
     2101 gcccgtacat cgggttgttg ccgtatcggc ccgcaccggc tgggaactgg atacgctggt
     2161 cagtgcgctc atgacagcac ttcccgacca tgccgccagt cccctgatga cccgcctgca
     2221 ggacgagctg cgcacggagt ctgtccggtc tcaggcccgt gaacagttta ccggtgcggt
     2281 ggaccggata tttgacacgg cggagagcgt ctgcattgcc tctgttgcac gcacggttct
     2341 gcgcgccgtc cgtgactcgg tggtctctgt tgcccgcgcg gtatggaact ggattttctt
     2401 ctgaacccgg tggtgatgta ccaggctgtt gttacagatt tttaactcca tgaaaacact
     2461 atgcaaacgc agccataccc gggttgtccc cttgcgttgt gtttctcaag ttaatagaat
     2521 gcatcgatcg ttactatcat atcgatcgtt ataaacgatc gtttgtagta agtgacctcc
     2581 tccgtggttg tcatgaaaat gcgtcttagg aaggttaaat aatgcacgaa aagaacatcg
     2641 ccctgctttg tgatgaagcc gaccgacttt tgcaactgaa cattaatctg ctccggcaaa
     2701 tggttgagga gccagatgtg ttatctgaca gtaagaacga aaacagactg ctttttgata
     2761 aacagaaagc actgaaaaga attgaggagc tggagggcga acaaatcaaa accgcccgca
     2821 gggagatggt gctggctgtt gtcggcacga tgaaagcagg caaatcaacc accatcaacg
     2881 ccattgtggg gcaggaaatt ctgcctaacc gtaaccgccc catgacctct gtaccgacgc
     2941 tcatccgcca cgttcccgga aaaactgagc cggttctcca tctggaacat attcagcctg
     3001 tccgcaattt attaatcaca ctgcaggaaa aactcgccac cccggcagga cagcaggtcg
     3061 cacagaccct gcagcaaacc ggggataccc gcgaactgct ggatattctg acggatgatg
     3121 gctggctcaa aaatgaatac cacggggagg aggaaatctt taccggactg gcatcgttaa
     3181 acgatctggt tcgtcttgct gcggcaatgg ggactgaatt tccttttgat gaatacgcag
     3241 aagtgcagaa actgccggtg atcgacgtgg aattcagcca tctggtgggg atggatgcat
     3301 gccagggaac actcacactg ctggataccc ccggccctaa tgaggccgga caaccgcaga
     3361 tggaagtgat gatgcgggat caactgcaga aagcctctgc ggttctggct gtgatggatt
     3421 acacccagat gaactcaaaa gcggatgaag acgtccgtaa agagcttaat gccattgctg
     3481 acgtatcagc cggccgcctg tttgtactgg tcaataaatt tgatgagaaa gaccgcaatg
     3541 gcgatggggc agatgccgta cgccagaaag ttccggcaat gctgaacagc gatgtgctgc
     3601 ccgcctcccg cgtttatccc ggatcctcac gccaggcata cctggctaac cgtgcgcttc
     3661 atgagttacg gaaaaacgga acccttcctg ttgatgaagc ctgggtcgat gattttatca
     3721 gaggtgcctt cggttgcatg aaaaaagaat acgtctgtaa agacagtgaa ctggcaactg
     3781 aaggggcgac agacctgtgg gaaggctcac ttatcgatca actgataacg gaagtcatac
     3841 agagctcaca ttccagagct gcggcactgg cggttgactc tgccgccgca aaactgatgc
     3901 agaatgcaga aaatatcagt gaatacctgt tgttacgcca tcaggggcta cagcaaagca
     3961 ttcagtcact gcagtcgcat atcaccagcc tgcttgcgga tatccgggaa atcgcggact
     4021 gtcaggagca gatgaccact gatgtcagaa tggccatgga ggagatcgat actaaaaccc
     4081 gggaattact gacgggggtc tgcacctcac tggaagagga gctgaatgac tatttcagaa
     4141 gcggtaaacg caaagaacag caaatgctgg aggaagaaga cgcagaacaa cgaaggtctc
     4201 agtccggatt atgggggaaa atttctcaat ggtccggtat caacaaccag ggccgggagg
     4261 attacaggaa acgagatttt gccccggaca gccccgaaat aaaattcagt gatcgcaggg
     4321 aagcccttga actgatgacg caaatcgaat cgaccgtgac cagcctgcac cgtgaggctg
     4381 aagcacagtt ccggcctgag ctggagaaaa tcgtcagcgg gattgaaaca ggttttcgtg
     4441 gcacggccct gtacgccaca gaaaacattg ccggtcgcat caatgcccgc ctggaggatg
     4501 agggctttac cgtaaaaatc agttttccgg cagtcagcca gttacagacc cggctcgcgg
     4561 taaaaataaa tctgagtgcg cttatggagg aaagaacgga gaccgtcacc cgtcgccgtc
     4621 ggcagagtgg cgtatgggga accgtttgtc gatggtttgg caccagtgac ctgggctggg
     4681 aaaactatga cgaggatgtg agtcgcagcg tgatcaatat caacaaggtc agagaggaag
     4741 ttatgtcact gacccgggca tatttcgggg agctgcaggc atccattgag caggatatta
     4801 accagcccgt ccgccaggaa atcgatgcct ttttctgcgc attcagggag aaagttgaac
     4861 aactgcgtaa cacgctgatt cagagctctg aagatcataa acgcgatcag caggcgcagg
     4921 aacgacttac cgggcgactt caggcattaa atgaaagggt tcctgagctc attactgaca
     4981 gtaaggcgct gagggaagag ctggagacaa tgctgtgact tcaccattta ttcaacaaat
     5041 tgctgataac cgggtatgtc aggtactcac ctgtcttcct gaaaaatttg tggttgattt
     5101 cgcaaacgga atcgatgttg cacaggagca tatccgcacg gccggagagc gcacgttctt
     5161 ccggcgttta aaagaggggc tgaccggcga aggcgctgca cggcagaatg ccatcaatgc
     5221 gtcgcttgca cagggcgttg aggcgtccct gcgctggctg acggagatga caacgtcact
     5281 tgccaccaca aactacgcga ttacccaggt aaacgacagg gtcagttcac tggtcagtga
     5341 tacagccagg ctggcgcatt attccgcaga tacgcgggag cagttactca ccctggccga
     5401 tcaggttcac cataaactga atcatcttga agaaaaactc caccgtgttg accaggttca
     5461 gcgggcgcag ttacatcttg agcagatatt ctcatggtgg agcgccgggc gatatgcgtc
     5521 attttcccct gccggacgtt gttatgtggc acttgaagag cttcgctggg gcgcgtttgg
     5581 tgatgtgata cgtcagagcg aaacaggcca ggttaaccag ctactggata ttctcagaca
     5641 taaagcatta acgcagatgg cacaggagag tggcggtagt gcaaccgtgc gtctgaacac
     5701 tctggactgg cttggtggtc agggccggga gcaggcagat aacgagtggc atgacgcgat
     5761 taactggctg ggagactggt gcagtgaaga gcagcatccg gtgatctggt caaccacgca
     5821 ggctgcagaa cacttaccgg ttcgcatgcc tcgcctctgc tctgcagaac gcctctctga
     5881 aagtatggtc gatgaaatat ttcagaaggg ggctgcatga gtacagaaat gaaaacgggg
     5941 ctggtgctgt ccggtggcgg ggcggtgggc gcttatcagg cgggagtggt taaggcactg
     6001 gcagagtgtg gtacacagat cagcatggtt tcaggggcca gcattggcgc attcaatggt
     6061 gccattatcg cggcctctcc cgatctgtca gaagctgccg tacgcctgga ggcgctctgg
     6121 gatcatctgg ggaataatca ggtgctgtcg gtaaacagat tggtttactt ttcattgctg
     6181 aaaaaattgt tccaggcaat gaacctctgc cagatccccg gacgtgcagg agcactgctt
     6241 acgacgcttc ttcgccatat atcgacaatc aacgggtttg acaatctgat ggctcagccg
     6301 ttgttgtcag atgagcccct gacagcgctg atggatcatt atcttgatac tgatgctctg
     6361 gcagacgggc taccgctgta tgtgtcgctg taccccacag aagggggcat gcaggatatt
     6421 attgactgca ttcgtgctga actgggtgtc ggaaccacga aaaacgccgt ttttcagcat
     6481 atccagagcc tgccccgcgg acagcagaaa gaggctctgc ttgcgtcagc cgcgctgccc
     6541 ctgctgttcc gtccccgtga ggttcagggg acaatgttcg gtgatggtgg tatgggagga
     6601 tggcgaaata tgcagggaaa tacccctgtg acgcctctgg tcgatgccgg atgcaatatg
     6661 gtgattgtga cgcatctgag tgacggttct ttatgggatc gccaggcttt tccggacacc
     6721 acaatccttg agatccgtcc ccggaaaagg ctgaaatatg caggtgatgg tggcaacagc
     6781 ggcggtctgc tcagttttac atcggcacat accgacgcct ggcgtcagca gggctatgaa
     6841 gacacgatgc tggcgatgga gcatatccgg aaaccgctgg cagcacgtca ggcactgacc
     6901 cggtcagagg cggtattgca gaaaagcctg gatataacgg aagaggcaga tttggcactg
     6961 agaaacgcga tagcccggat taaataaaga cgccccaaag aaacgccacc gcctgtggtg
     7021 gtgtttttcc tgtctggtgt tattgaaaat attttcagat atatattacc tgagagaaag
     7081 ttttacccct cacctgtcaa ttcactcact ggaaatttaa ttatgaaatt acctgtaaag
     7141 ttattaatga gccttatatc tctggtcagc gttattgcac gtgccgggga atataaaaat
     7201 tacagccggg atgaaataaa atactggcga tatacatcat acaagggggg gaaacttccg
     7261 gaaggtttca ctgatgagaa attttccagc gccatttaca acggaagaat atttacaatg
     7321 aaacgtttac atacccttat gttatttctg gcggttctgt tcacgggctt taactttaac
     7381 gcagaagcgg ctaccgtaaa acaagctctt agttgtaacc cggaagcctg ggctgaacaa
     7441 cctggagcgt gtccatcaac gtacgagttg tacgaaggtg acgctaccta caaagccgct
     7501 atcgacaaag cattaaaacc ggtcggactg agcggcatgt tcggtaaagg cggctatatg
     7561 gatggccctg gtggaggtat cacgccagta aacattaacg gtacagtctg gttccagggc
     7621 gacggttgca aagccaatac ctgcggctgg gactttatcg taacactcta taacccaaaa
     7681 acccatgaag ttgttggcta ccgttacttt ggtttagatg acccggccta cctggtttgg
     7741 ttcggtgaaa ttggcgtgca tgaattcgcg tatctggtga aaaactacgt agctgcggtt
     7801 aactaattta aggagtctat catgaaaact tttatcaaaa ctttactcgt tgctgtaact
     7861 attctgttct ctgtcttcgc tactgcgaaa caagtaaaac tgccaaataa catcaaatac
     7921 gttaatacta cagaagcgtt ttcctgtacc gaaattgacg gtatgaattg ccagacaaag
     7981 aatcagttca actataaaga taacagctat gttttcgtgc ttgaacgtgg tggtgcctgg
     8041 tgctacgact acactgtctc ggtagttaac ctgaaaaccg ggaaagcaca aatgattgaa
     8101 tacggggaca atcaactgtg ctcaggtagc aacaaaccgt tcttcgaaat caaaaatggc
     8161 gtaccgacgg taggagtcat cgacacatcc ggaaaacctg tcgttgtagc ccaggacaaa
     8221 cttaaaatct aaggagtaaa ccatgcgttt ctctgcacat tattaatatc aaaggtaaat
     8281 acagccccag ccattgttac cggaaagccg cttaatgcgt ggctcgccag tgaaaggcct
     8341 ctcagacaat aaaccttatt catttcaccc gtcaggccgc gtcttctccg ggagacgcgg
     8401 cttttttcat ttattgcacc agtaaatctt aaccaccgat aaggagcaaa gtatgcgatt
     8461 agcaagtcgt tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacatga
     8521 agaactgata cgccatgtac ccagtatttt tggggaagac cggcacacct cccgcagtga
     8581 acggtatgcg tacattccca ccattaccgt cctggaaaat ctgcagcggg aaggctttca
     8641 gccgttcttc gcctgccaga cccgtgtgcg cgacccgggc cgccggggat acacaaaaca
     8701 catgctgcgt ctgcggcggg ccggagagat aaacggagaa catgtccctg aaatttttct
     8761 gctcaactct catgacggta cctccagcta ccagatgctg ccgggttact tcaggttcgt
     8821 ctgccagaac gggtgcgtct gtggccagtc tctggaggaa gtgcgtgttc cacaccgggg
     8881 aaatgtagtg gagaaagtta tcgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat
     8941 agaggagaag cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct
     9001 ggcacaggcg gcactgactt accgttatgg tgacgaacat cagcccgtca ccaccgccga
     9061 cattctgacg ccgcgacgcc gggaggatta cggtaaggac ctgtggagcg catatcagac
     9121 catccaggag aatatgctga aaggcgggat ttccggtcgc agtgcaaaag gaaaacgtat
     9181 ccatacccgg gccattcaca gcattgatac cgacattaag ctcaatcgcg cattgtgggt
     9241 gatggcagaa acgatgctgg agagcctgcg ctgatgccgt ttccctgagt gagggaatca
     9301 ttcagctttc gttcctgaaa aacacacatt tatttgttat ttaaggagtt atctcatgaa
     9361 aaccgtttct cagaataccc ccacaattta ttcagctaca acaccagaga ataatccgcc
     9421 tcagttggtt gccagcctcg tccctgatga acagcgcatc agcttctggc cgcagcattt
     9481 tggcctcatt ccacagtggg tgaccctgga gccccgtgtc ttcggctgga tggaccgtct
     9541 gtgcgaagac tactgcggtg gtatctggaa tctgtacacc ctgaacaacg gcggggcatt
     9601 tatggcaccc gaaccggatg acgatgatga cgaaacatgg gtactgttca atgccatgaa
     9661 cggtaaccgc gcagaaatga gcccggaagc cgccggtatt gccgcctgtc tgatgacgta
     9721 cagtcatcat gcctgtcgta cggagaatta tgccatgacg gtccattatt accggttgcg
     9781 ggattacgcc ctgcagcatc cggaatgcag cgccattatg cgcatcatcg actgaatgga
     9841 gagaagcaca atgcaacaac tttcctttct gcccggagag atgacgcccg gcgagcgcag
     9901 cctcattcaa cgggccctga aaaccctgga ccgccatctt catgaacccg gcgtggcctt
     9961 cacctccacc cgtgcagcac gggaatggct gattctgaac atggcgggac tggagcgtga
    10021 agaattccgg gtgctgtatc tgaataacca gaatcaactg attg
//
LOCUS       DAPGWD010000150        10014 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPGWD010000150 DAPGWD010000000
VERSION     DAPGWD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.151"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..569)
                     /locus_tag="RHX88_003620"
     CDS             complement(<1..569)
                     /locus_tag="RHX88_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDV2232267.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAV"
     gene            836..2335
                     /gene="ansP"
                     /locus_tag="RHX88_003621"
     CDS             836..2335
                     /gene="ansP"
                     /locus_tag="RHX88_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDV2232268.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2450..3511)
                     /locus_tag="RHX88_003622"
     CDS             complement(2450..3511)
                     /locus_tag="RHX88_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDV2232269.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3753..5855
                     /locus_tag="RHX88_003623"
     CDS             3753..5855
                     /locus_tag="RHX88_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV2232270.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5891..6556)
                     /gene="mcbR"
                     /locus_tag="RHX88_003624"
     CDS             complement(5891..6556)
                     /gene="mcbR"
                     /locus_tag="RHX88_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDV2232271.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6754..7791)
                     /gene="curA"
                     /locus_tag="RHX88_003625"
     CDS             complement(6754..7791)
                     /gene="curA"
                     /locus_tag="RHX88_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDV2232272.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            7972..8490
                     /gene="mddA"
                     /locus_tag="RHX88_003626"
     CDS             7972..8490
                     /gene="mddA"
                     /locus_tag="RHX88_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDV2232273.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8487..8936
                     /locus_tag="RHX88_003627"
     CDS             8487..8936
                     /locus_tag="RHX88_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2232274.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8937..9170)
                     /locus_tag="RHX88_003628"
     CDS             complement(8937..9170)
                     /locus_tag="RHX88_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDV2232275.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9256..9429)
                     /gene="ortT"
                     /locus_tag="RHX88_003629"
     CDS             complement(9256..9429)
                     /gene="ortT"
                     /locus_tag="RHX88_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDV2232276.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9624..9719
                     /gene="yncL"
                     /locus_tag="RHX88_003630"
     CDS             9624..9719
                     /gene="yncL"
                     /locus_tag="RHX88_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDV2232277.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2410 a         2448 c         2452 g         2704 t
ORIGIN      
        1 catacagcat cagcgatcgc gctgatattc tgcgcattat cctgaaacca ttcatgcccc
       61 ggcccccatg tgcgcatggt gcagagataa cagtccacca gcgttagttg ctcaccaaag
      121 gcataaggtt cagcggtcaa ttgtgaattc agccagatat aaagtgattt gcgatattca
      181 attacattct tctttaactg ttcaggtgcg tcagatgccc aacgctcagg gtaatcggcg
      241 aaagtgaatg ttggatagac attagcaacc agccagacca atagccgttg aaactgctga
      301 cgttcggcgc gcccaaccgg tggcgcaagg tccgggcgac gatcgagaac catcaatgca
      361 atcgccgctg tctccgtcat aatctcgtca ttttccagcg ccagtgtcgg aacctgacac
      421 aacgggttca gggttttcaa taactcgcgt gaagctccct cgtggtcaaa accactgaca
      481 tcaacgaact gataaggaat atcagccagt gttagcatca gctcactgat tgtagagccc
      541 catccgggta cgccatacac cttaatcata tgcccctcac aatcttttaa agattaagtg
      601 taggacagta cacaggttaa ccagcaaaag gatttgtcat tttttcgtca gttgtattaa
      661 tccccttcag gattgttagg ggatgttttg catcgtttat cgataacggg taggatgctg
      721 cgccgatagt tttataccca taaagaataa tggtgataac tatcatcgcc aggatgaata
      781 aacattgttc atggcaactt atatgacttt ttcattaaag caatcaggga gagcaatgag
      841 taaacacgac accgacactt cagatcaaca cgccgcgaaa cgccgctggc ttaatgccca
      901 cgaagagggg tatcacaaag cgatgggcaa tcgccaggta cagatgatcg ccattggcgg
      961 cgcgattggc accggcttgt ttttaggtgc aggagcccga ctgcaaatgg cgggtccagc
     1021 attggcactg gtttatttaa tttgtggctt gttttcgttt tttattctgc gtgcattggg
     1081 tgagctggtg ctacaccgcc cttccagtgg cagttttgtc tcttatgccc gtgagttttt
     1141 gggtgagaaa gccgcttatg ttgctggctg gatgtacttc atcaactggg cgatgacggg
     1201 gattgttgat attaccgccg tcgctctgta tatgcattac tggggtgcgt ttggcggcgt
     1261 gccgcagtgg gtctttgcgc tcgctgcact taccatcgtt ggcaccatga atatgatcgg
     1321 tgtgaaatgg tttgcggaga tggagttctg gtttgcgctt attaaagtgc tcgccattgt
     1381 gacctttttg gtcgtgggta cagtgttcct cggtagtggt cagccgctgg atggcaacac
     1441 cactggcttt catttaatta ccgataatgg cggcttcttc ccccacggtt tgctgcctgc
     1501 tctggtgttg attcagggcg tagtgtttgc ttttgcctcc attgaaatgg tgggtacagc
     1561 tgccggagaa tgtaaagatc cgcagaccat ggtgcctaaa gcgattaaca gcgtgatttg
     1621 gcgtattggc ctgttttacg tcggctccgt ggtgttgctg gttatgctat tgccgtggag
     1681 cgcgtatcag gcggggcaaa gtccgttcgt gacgtttttc tctaaactgg gtgtgccata
     1741 tatcggcagc attatgaaca ttgtggtgct gaccgctgcc ctctccagcc tgaactcagg
     1801 tctgtactgc accggacgta ttctgcgctc aatggcgatg ggcggttccg caccgagttt
     1861 tatggcgaaa atgagtcgtc agcatgtgcc gtatgccggg attctggcga cactagttgt
     1921 gtatgtcgtc ggcgtattcc tcaactatct ggtgccgtcg cgcgtatttg agattgtgtt
     1981 gaacttcgcg tcgctgggaa tcatcgcttc atgggcgttt atcatcgtgt gccagatgcg
     2041 cctgcgtaaa gcaattaaac aaggcaaagc agcggatgtc agttttaaac tgcctggcgc
     2101 gcccttcact tcttggctga cattactgtt tttactgagt gtcctggtgc tgatggcgtt
     2161 cgattacccg aacgggactt atactatcgc ggcgctgccg attatcggta ttctgctggt
     2221 tataggctgg tttggtgtgc gcaaacgcgt tgctgaaatt cacagcactg cgccagtcgt
     2281 cgaggaagat gaagaaaaac aggaaattgt gtttaagcct gaaacggcga gctaattgca
     2341 aaattgcccg gctacgtgcc gggcaaattt agattactga cgaaggtgtg ccgcatgcgg
     2401 cgtgataaaa gtcaggcaag tctctccgtt ttcaccctcg ccacatctat tacagcgcaa
     2461 tacgaatcac atcgtccggc tgggtagctt cctgctgttt agtggatttt tgtttcacac
     2521 tgacatacag cgttttgcca tcggcagaca gcgccaggct gtttggatga gtcggcgtat
     2581 cgaacgtttt caccacttta tagcttttcg cgtcaatcac actgacttta cctgcctgac
     2641 gatgcgttac gtaggcttca ttacgcgctg ggttaaacag cacagccagt gattctggtg
     2701 ccgcaacctt cgccagaata ttgccattac gggtatcgac cactaacact tctgcggctt
     2761 tagaatcggt gataaatgca cgctgcctgg cggtatcaag gctaatgttg ataaagaagt
     2821 gctctttgcc gtcatccagc agctttttac ggctgaggat tttattgtcg gcggtgtcga
     2881 tggtaatcaa ttcgccgtca gcgttagtgg tgtaaagacg tttgcctttg ctatccagcg
     2941 ccagaccggt actcatttta ccggtgttct ggatggcggt tttcagtttg atattctcgc
     3001 catcaacgac ccaaatcacg ctctctttac caataccact gatgtaaacg gtgttcgtgg
     3061 catcatcagc taccagctca cgcggttgca gcgggcgcac ctcttccgtg cgcttacgat
     3121 catccagcac cagacggcct ttcacctcgc ccgttttggc atctatcgcc gtgaccgcgc
     3181 tgtttacggt gttaccaaac cacaacgtct gagtcgtgtt attgatggtg gcaccaaacg
     3241 gcttgagatc gttatggatc gcctgcgtca cttccagagt gaccggatca agacgataaa
     3301 ccacgccacc tttatccagt ttgcggcttt gcgaagtggc gagccacagt gcgttttctt
     3361 gctggctata agccatttcg taggcacctt tacctaccgc tttacgcagc atttcttctg
     3421 cggcctgcgt actgaatgat gaagcaacaa gcaatgaacc taacagtaat gaaccacgca
     3481 ggcgcgatga aaacagatga cgtaaatgca tgacgactcc ctttgatgaa ccgatatttt
     3541 tgctgcttca caatgcttcg ctaagcgtaa tcatcgttac gctcttgtta taaatgggaa
     3601 tggtaatcat tattttctca aatgtggagc atttcttgcc gttacgacta caattttctc
     3661 aattacttaa cgtatcgtgc cgtttcgttt tccataaatt tacaatttca ttaccataac
     3721 ggcgttttcc cattcttaac ctggcttttt tcatgaagat tttttccgtc cgacagaccg
     3781 ttttgcccgc actgcttgtc ctttcccccg ttgtttttgc cgctgatgaa cagactatga
     3841 ttgtcagtgc cgcaccgcag gtggtttcag aactggatac cccagcagca gtaagcgtgg
     3901 tggatggcga ggagatgcgc ctggcaacac cgcgcattaa cttgtccgaa tcactgaccg
     3961 gcgtgcctgg tttgcaggta caaaaccggc agaactatgc gcaagattta cagctgtcga
     4021 ttcgcggatt tggctcccgc tccacttacg gtattcgcgg tattcgcctg tatgtggacg
     4081 gtattcccgc caccatgccc gacgggcaag ggcaaacatc caacatcgat ttaagcagtg
     4141 tgcaaaatgt ggaagtgctg cgtggcccct tctctgccct gtatggcaac gcgtctggtg
     4201 gggtaatgaa tgtcaccacc cagaccggac aacagccacc aaccattgaa gccagtagtt
     4261 actacggcag ttttggcagc tggcgctatg ggctgaaagc aacgggcgca acgggagacg
     4321 gcacacagcc tggcgatgtc gattacaccg tctcaaccac gcgttttacg acccacggct
     4381 atcgtgacca tagtggcgca cagaaaaatt tagctaatgc caaactgggc gtacgcattg
     4441 atgaagccag caaattaagt ctgattttca atagtgtgga tatcaaagca gatgacccag
     4501 gtgggctaac caaagcagaa tggaaggcta atccacaaca agcgcctcgt gcagaacagt
     4561 acgacacgcg aaaaaccatc aagcaaactc aggctgggtt gcgctatgag cgtagcctga
     4621 gcgcgcaaga tgatatgagt gtgatgatgt atgccggaga gcgagaaacg acccagtacc
     4681 agtcaatccc gatggccccc caacttaacc cgtcacatgc gggcggcgtg attaccctgc
     4741 aacgccatta ccaggggata gacagccgct ggacacaccg tggtgaactg ggcgttccgg
     4801 tcacgttcac taccggcctg aactacgaaa acatgagtga aaaccgcaag ggctacaata
     4861 acttccgcct gaatagcggc atgccggagt acgggcaaaa aggtgagttg cgtcgcgacg
     4921 aacgcaatct gatgtggaac atcgatccct atttacagac gcagtggcag ctgagcgaaa
     4981 aactgtcgct ggatgctggc gtgcgctaca gctccgtgtg gtttgattcc aacgaccatt
     5041 acgttactcc gggtaacggc gatgacagcg gtgatgccag ttatcataaa tggctacctg
     5101 ccggttcgtt aaaatatgca atgaccgatg cctggaatat ctatctggca gccgggcgag
     5161 gttttgaaac gccgacgatt aatgagctgt cttatcgtgc tgatgggcaa agcggtatga
     5221 actttggttt aaaaccatcc accaacgata caattgagat cggcagtaaa acgcgtattg
     5281 gtgatgggct gcttagtctc gcattgtttc agaccgacac tgatgatgaa attgttgtcg
     5341 atagcagtag cggtgggcgt acgacttaca aaaatgccgg aaagacccgt cgtcaaggcg
     5401 ctgaactggc atgggatcaa cgtttcgcag gagattttcg cgtaaacgcg tcctggacct
     5461 ggcttgatgc gacctatcgc agcaatgttt gcaatgaaca ggattgtaac ggtaatcgga
     5521 tgccagggat cgcccgtaat atgggctttg cgtcgatagg ttatgtaccg gaagatggtt
     5581 ggtatgcagg cacggaagcg cgttatatgg gcgatattat ggcagatgat gaaaatacgg
     5641 caaaagcgcc gtcttatact ctcgtcggct tattcaccgg gtataaatac aattaccaca
     5701 atttaactgt ggatttattt ggtcgtgtcg ataatttatt cgataaagaa tacgttggtt
     5761 ctgtcattgt caatgagtca aacgggcgat attacgaacc ttcgcccgga cgaaattatg
     5821 gtgtcggcat gaatattgcg tggagatttg agtaattacc ttgatgcccg gtatttgccg
     5881 ggcatttact ttaacgattg tattgctggt ataaaatagc aatattttgt tgcataattt
     5941 cagcaagaca atgtctgcat ccctctttgt cttttgcttt taacgcagcg agtaacagat
     6001 gatagttttc tatatgctcc cgtaattccg ctggattaat cgcttcatag agataatgta
     6061 aaccaggccc catcctgacc cacagttgct caatcatctc acacaggatg ggcatatttg
     6121 agcgatgata aatcgctaag cgaaatagcc tgtttacatt aatgatttgt tccatgtcgc
     6181 ccttttcttg cgcctgctgt aatttctcga gcaattcctg gagttccgcc aggtcttgcg
     6241 gggtgaggtt ttcaacagcc agagcaactg ccattaattc cagttcgtag cggatccgat
     6301 tgatttcgtc taattgacgt ttcctcactt ccggaactgt aaatgcttgt gcaggtgcga
     6361 cagaaagcgc attcaccgaa accagacgta ataatgcttc acgcacaggt gtaatactca
     6421 tacctaattg ctccgccaga tttttagtaa tcaggcgtgc gccaggtttt aatgcgccaa
     6481 tactaagctg atgtttcagg tcgttctcaa cctgcagagt caaactgaca tgtttcattt
     6541 ttcccgttcc aggcatttta attccttcgc gtgttttctg tccaaccctg cctgcatggc
     6601 agaaggagtt cacctggtgt attaaagtga ataatcataa accactcacc tttgttgtta
     6661 ttagcagcag aacaatgacg tggttagtta attaaagctg aaaataagct ttttgcctgg
     6721 atttttatgg cggcttacgc cgccgttgaa actttaatca tcacccgcga cgcggatcac
     6781 cactttgccg aagtttttac ccttcagcag gccgataaac gtctgtggcg cattctctaa
     6841 accatcagta atatcttcgc ggtagtggat tttatcctct ttcacccatt gccccatctc
     6901 cttctgaaac tcatggatgc ggtgaccata atcctgagcg ataataaacc cttgcaagcg
     6961 aatacgtttt ttcagcaccg ttgccatcaa caggggtaaa cgatccggac cgggtggtag
     7021 ctctgtagcg ttatagctgc tcactaagcc acaaacggga atgcgcgcag atgtattaag
     7081 taatggtagc actgcatcaa ataccttacc gcccacgttt tcataataga tatcaatacc
     7141 ttttgggcac gctttcacca gttgttcggc aaaatcatcc gcgtggtgat caagacaaac
     7201 atcgaagccc agcacctcga tagcatggcg gcatttttcc gcgccaccgg caacccccac
     7261 caccctgcaa cctttaagtt tgccgatttg ccccaccgtt gctcccacag gacctgtcgc
     7321 cgcagctacc accaacgttt cgccctcttt aggctgaccg atatccagca gccccatata
     7381 agcggtaaag cctggcatcc ccagcacacc cagcgaccac gatggatttt gcggatgatc
     7441 gccaagtttc accagatcat caccactgga tatgtcatag tcctgccatc cactgtagcc
     7501 cagcacccag tcgccaggct gataatcagg atgattcgac tccacaacac ggctcaccgt
     7561 gccgccgacc atcacgccgc caatatcaac aggcggtgaa taagatggct catcgctcat
     7621 acgtccacgc atatacgggt ccagggacaa ataaactgtg cgcagtaaca cctgtccttc
     7681 acccggtgtg gcgacatcat cttcttcaag acggaaattc tccggaaccg gtgcgccatg
     7741 tggacgcgag gccagaaccc aacgtcgatt acgctgcttt tgttgcccca taatgctctc
     7801 ctgtgctttt ggcatgaatc catcaatagt agcaggctca cgggattggt tttgtttgca
     7861 gccgtcacat cccctctgca acacgcaata atttcatcga ggaatccgtt gcttcgggct
     7921 gttttcgata gtattgataa catcatcatc attattttat cgaggccaga catgtccatc
     7981 cgttttgccc gcaaagccga ttgtgctgcc attgcggaaa tttataacca cgccgtgttg
     8041 tatacggcgg ctatctggaa tgaccaaacg gttgatgcag ataaccgcat tgcctggttt
     8101 gaagcgcgga ccatagcagg ttatccggtg ctggtgagtg aagaagatgg cgtagtgacg
     8161 ggatatgcct cgtttggcga ctggcgtagt ttcgatggtt ttcgccatac cgtggaacat
     8221 tcggtttatg tccatcccga tcatcagggc aaaggtctgg ggcgtaaatt gttaagccga
     8281 ttgattgatg aagcgcggga ttgcgggaag catgtcatgg tcgccgggat cgaatcgcaa
     8341 aatcaggcct cgctgcatct gcaccagtcg ctgggatttg tcgtcaccgc acaaatgccg
     8401 caggtgggca ctaaatttgg gcgctggctg gatctgacat ttatgcagtt gcaactcgac
     8461 gaacgcactg aaccggacgc gattggatga atcagtcgct cacccttgct tttttgattg
     8521 ccgccggaat tggtctggta gtccagaaca cgctgatggt gcgcatcacc cagacctcct
     8581 ctaccattct catcgccatg ttgctgaact cactggtggg gattgtgctc tttgtttcta
     8641 ttttatggtt taaacaaggc atggcggggt ttggcgaact ggtgtccagc gtgcgctggt
     8701 ggacacttat acccggcttg ctgggatcgt tttttgtctt cgccagtatc agcggatatc
     8761 agaatgtggg agcggcgaca actattgcag tactggtcgc cagccagcta attggcggtc
     8821 tgatgctgga tatcttccgc agtcatggcg taccactacg ggcgttgttc ggcccaattt
     8881 gtggcgcaat tttacttgtg gttggcgcct ggttggtagc cagacgctca ttttaatcag
     8941 aggatggtgc cgcctttggt taactgttcg tggcgcgctt ccatatcttc tttatgcttg
     9001 cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct gggtgtagcg atcttcccga
     9061 cttaactccg cgtcatcgct cagggcgatt aataattcgt tcatatgggc aatgacgctc
     9121 tcttcaatcg cggcatccac gcgcgcgatg acttcatcca gatgcgacat actttctcct
     9181 taaccacttg tgaaatttat cgattcatcc aaatgatata agtctgcgtg gtgccgtcaa
     9241 gccagtgagt aagatttaaa acagtgaaaa caacagcgcc accgggaaac tcatcggcca
     9301 tgttgccccc accagaaagg cgcttaagaa acggatgcgt ttcttatcgt gagaaagaaa
     9361 ccaggtgata agaaatgcga ttgctgccat aaccgcataa aaaacaagca tatgttgata
     9421 gagagacatg tacgggcgtc cagccagatg aataaaacgc gcgcaaatat gcgtcatttt
     9481 ttgacgtaca tcaagttttt tcaccagttt gagaaaaaat catcaaacag caacttgccc
     9541 ttatccatct tcagaatgag tattattgcg gattttctta acccgtacta tacacatagg
     9601 ggcaatgata aaaggtggca aaaatgaatg tttccagtag aactgtagta ctgataaatg
     9661 tctttgctgc tgttggtttg tttactctta tctctatgag atttggctgg tttatttgat
     9721 gtagtgattt tgcacgcagc acgtactgtc agagaaatca actctctgac agtacgcaag
     9781 atcttctcat actctccagg tttacaacat tatccttaat atttacttct ttttgctggc
     9841 atctacattg aaatgagtag aaaacatccc cctactgtcg tctatacaaa cagcttactg
     9901 attcaaacgg aatcttgttt tcacattttt ctatcatgaa tactttatac gcgcgggagt
     9961 ctataaatgc tttatacttt caaggaataa cgtaaatata aaaagatggc gtaa
//
LOCUS       DAPGWD010000151         9923 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPGWD010000151 DAPGWD010000000
VERSION     DAPGWD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.152"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            88..225
                     /gene="hokA"
                     /locus_tag="RHX88_003631"
     CDS             88..225
                     /gene="hokA"
                     /locus_tag="RHX88_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDV2232278.1"
                     /translation="MKKCTAPICFTLLFFTWMIRDSLCELHIKQGSYELAAFLACNLK
                     E"
     gene            complement(413..625)
                     /gene="cspA"
                     /locus_tag="RHX88_003632"
     CDS             complement(413..625)
                     /gene="cspA"
                     /locus_tag="RHX88_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDV2232279.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(906..1196)
                     /locus_tag="RHX88_003633"
     CDS             complement(906..1196)
                     /locus_tag="RHX88_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDV2232280.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1630..2340
                     /locus_tag="RHX88_003634"
     CDS             1630..2340
                     /locus_tag="RHX88_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDV2232281.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2390..3364)
                     /gene="ghrB"
                     /locus_tag="RHX88_003635"
     CDS             complement(2390..3364)
                     /gene="ghrB"
                     /locus_tag="RHX88_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDV2232282.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVDQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3468..4127)
                     /locus_tag="RHX88_003636"
     CDS             complement(3468..4127)
                     /locus_tag="RHX88_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDV2232283.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4280..6613
                     /gene="bisC"
                     /locus_tag="RHX88_003637"
     CDS             4280..6613
                     /gene="bisC"
                     /locus_tag="RHX88_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDV2232284.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMGWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTILMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALEKPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLEMFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6582..7022)
                     /locus_tag="RHX88_003638"
     CDS             complement(6582..7022)
                     /locus_tag="RHX88_003638"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2232285.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRECIPLVRNAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7019..7582)
                     /gene="tag"
                     /locus_tag="RHX88_003639"
     CDS             complement(7019..7582)
                     /gene="tag"
                     /locus_tag="RHX88_003639"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDV2232286.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDIERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7740..8438
                     /locus_tag="RHX88_003640"
     CDS             7740..8438
                     /locus_tag="RHX88_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2232287.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEL
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8667..9869
                     /locus_tag="RHX88_003641"
     CDS             8667..9869
                     /locus_tag="RHX88_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2232288.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2357 a         2400 c         2565 g         2601 t
ORIGIN      
        1 taagctagaa tagccacggg tgcttgagac tgtttgtctc aggtattcac cgaaaggcag
       61 acagagaaaa gccccacctg actataaatc aaaaagtgta ctgcacccat ttgtttcacg
      121 cttttatttt tcacctggat gataagagat tcactgtgtg aattgcatat taaacagggg
      181 agttatgagc tggcggcatt tttagcctgc aatttgaaag agtaagagtc ttcggcggga
      241 agttattccc gcctttttta cggcgttgcg cattctcatt gcacccaaat ttatttttca
      301 caaaaataat actagatttt attacgcgat cgattattta tttcctgaaa aacaaataaa
      361 aaaatccccg ccaaatggca gggatcttag attctgtgct tttaagcaga gattacaggc
      421 tggttacgtt accagctgcc gggcctttag cgccgctttc gatggtgaag gacactttct
      481 gaccttcgtc cagagatttg taaccatcgt tctggatagc agagaagtgt acgaacacgt
      541 ctttagagcc atcgtcagga gtgatgaagc cgaagccttt gtcagcgttg aaccatttta
      601 cgataccagt cattttaccg gacatagtgt attaccttta ataattaagt gtgcctttcg
      661 gcgatatggc gtgctttaca gattttgaag cgttaaagga atgtgcacta cgaggggtat
      721 caacgataac tcttgaaggg acttgcctta ctacactgct ttaatggtct gtacgtcaaa
      781 ccgttgatgt gcattaagcc acgcattggc gggtgatgca acaattattt ttcatattta
      841 tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt aaacagcaat aaagtggata
      901 aaagtctatt ccatcaactg cttacttaat gccgggttgg cttgaatcaa acgcatcaat
      961 tttagttcgg cacttgaagg cttcacgcgt ctggattccc attcctttac catggcgact
     1021 gatacgccca aaacccgggc gaaatcatcg atttttaatc ctgtcccttt tcgtaactgc
     1081 tcaatttcgg tacaggacgt tgttctgtgc gtcagggtaa ttttctgcgt ttcatcttta
     1141 aaaacaatct gttccaggct gctcaacagc tcatgcattg gatctttata ttccattgaa
     1201 aactcctcaa atcacactgc gggatcgtga attgcatcga agctcattaa gaatagtcgg
     1261 gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg gctatgattc gattcagcca
     1321 gtcatttagc gtgacttata gccacctaac gatctgataa atgccgatat atctcttacc
     1381 actcctggtt tgtaaatatt tcgaaaatct aaccttctgc gaaggcgtaa atctctggct
     1441 acaaaaaagg caacggcaat atcatttttt aacaatgaaa caacaaatta accttgtttc
     1501 attttctcca tgattttacc aggaagatta cgtagaaagg gtatcctgca agcctgtcct
     1561 ggactatcct tgtcacgtca gacacgcgtg tgtcgttgtg cgcttttttt gggtgaaagg
     1621 agtaagaaaa tggcgacagg aaagtcctgc tctcgctggt ttgcgcctct tgcggcgtta
     1681 ttaatggtag ttagcctgag tgggtgtttt gataaagaag gcgatcagcg taaagcgttt
     1741 atcgacttcc tgcagaatac agtgatgcgt agcggtgaac gtctaccaac cctgactgcc
     1801 gatcagaaaa aacagtttgg tccttttgtc tctgattacg cgattctgta tggttattct
     1861 cagcaggtga atcaggcgat ggattccggt ctgcgcccgg ttgtagacag cgttaacgca
     1921 attcgcgtac cgcaggatta cgttacgcaa agcggtccgc tgcgtgaaat gaatggttcg
     1981 ctgggtgtgc tggcgcaaca actgcaaaat gcgaagttgc aggccgatgc tgcgcactct
     2041 gcgctaaaac agagtgatga cctgaaaccg gtctttgatc aggcattcac caaagtggta
     2101 acgacgccag ctgatgcatt gcaaccgttg attccggcgg cacaaacttt cacgcaacaa
     2161 ctggtgatgg ttggagacta cattgcccaa caaggtactc aggtgagctt tgtagcaaat
     2221 ggcatccagt tcccgacttc acagcaggca agtgaatata acaaactgat tgcgccatta
     2281 ccggcacagc atcaggcgtt taatcaggcc tggactacag cagttactgc aacccaataa
     2341 agagtaaaag cccgagcgat tatcgggctt tactccacgc agtcgcggct tagtccgcga
     2401 cgtgcggatt cacacagttc ttctcaacct ttccttgtaa cgcatcaatc aaattatcca
     2461 cggcacaggc ggccatgcca taacgcgtct catgggtggc agatccaata tgcggtactg
     2521 cgacgacgtt ggccattgag agcaacggcg aatctacgga aagtggctct tgttcgaaga
     2581 catccagccc ggcggcgtga atttcgcctt tctgcaatgc agcaatcagt gcattttcat
     2641 caaccaccgg gccacgtccg gcattaatga aaatggcgga ggatttcatt ttggcgaatt
     2701 gttctgcgcc aaacagatga tacgtctcat cagttaacgg caggatcagg caaacgaaat
     2761 ctgactcttg taacagagta tccaaatcgc agtagcgggc gttgaagcgt tcttctgctt
     2821 ctttatggtg gcggcgcgcg ttatagagga tgggcatgtt gaaaccaaag tgcgcacgtt
     2881 gtgccagcgc cataccgatc cgtcccatcc cgacaatgcc cagtgtttta tggtgaacgt
     2941 cagtgccgta ccagtccggg cctatgctcg cggtccattc gcctgctttt acccgttctg
     3001 ctacctccac aacccgacga gcggtagaca acaccagcgc catcagcgta tcggcgacgg
     3061 tttctgttaa tacggttggc gtgtgcatca gcagaatttt tcgggcggta agagcatcga
     3121 catcaaaatt gtcatagccg acggagatcg ttgatgtggc acgcagtttc ggcatttttt
     3181 ccagcaatgc ggcatctaca ttctcgtttg aacccagtaa accttcagct tctgcaaaaa
     3241 ttgctgcatt ttgatcgacg gtttgtgggc tgaggtttgc cacctggtga acggtgaaat
     3301 gttcttgcag gcgttgcagt aaatcatcag gtaaggcttt gtagaggata acggacggct
     3361 tcatgcttct ctccattcac tgataaggcg atcaccttag cctgaaatat gatgaaggaa
     3421 aactgaagaa tgccaggtga tttctgcatc acctggcatg aaagggatta cagcgggctt
     3481 aaggtaattt ctacacggcg gttttgcgcc ttaccttctg cggtgctgtt gctggcgatt
     3541 gggttagccg ggccaaggcc ctgagtacgg atgcggctgg cgtccacgcc ctgggtgatc
     3601 aacgcgctgg caacggaatc cgcacgctgc tgggagagac gcatgttcag atcgtgacca
     3661 cccgtgctgt cggtataacc aatcacgtta accgccgttt tcggatactc tttcagcacc
     3721 attgccacgc cggtcagggt gttagcgccc gcaggtttca gggtcgcgct gctgctgtcg
     3781 aaggtcacat tgttcggcat attgaggata atgttatccc cgctgcgggt tacactaaca
     3841 ccagtgccgc gcattttgtc acgcagcttc gcttcctgca catccatgta ataacccaca
     3901 ccgccgccca gagctgcgcc tgctgctgcg ccaatcagcg cgcctttacc gcgatctttc
     3961 ttcgaagaag agagcgcacc aatacccgcg cccacgagag agcccagacc tgcgccgata
     4021 gcagatttac ctgcttcgcg ttcgccggtg taagggttag ttgtgcagcc agataccgcc
     4081 agagcgccac tcactacggc ggcaataaga taaacacgtt tcttcattgt taatccttaa
     4141 taaccttttt attctttgcc acgggttccg tggcgggaga ttatgccgcg tgaacatgaa
     4201 gattattcct gggaatactc ggaaatttgt aagtaatatt taactgctca atacatctaa
     4261 cctttcagga gtcttcggtt tggccaactc atcctcacga tattccgttc tgactgccgc
     4321 ccattgggga cccatgctgg ttgaaaccga cggcgaaacc gtgtttagct cgcgtggtgc
     4381 gttagccaca ggaatggaaa actccttgca gagcgcggtt cgcgaccagg ttcacagcaa
     4441 tacgcgggta cgatttccaa tggtgcgcaa aggctttctc gcgtcaccgg aaaacccgca
     4501 aggcattcgt gggcaggatg aatttgttcg cgtgagttgg gatgaggcgc tggatcttat
     4561 tcaccaacaa cataaacgca ttcgtgaggc ttatggtccg gcatcgattt ttgctggttc
     4621 ctacggctgg cgttcaaacg gcgtgctgca taaggcctcg acattattac aacgctatat
     4681 ggcgctggca ggcggttata ccgggcatct gggggattat tcgaccggcg cggcacaggc
     4741 gatcatgccg tatgtcgtgg gtggtagtga agtttatcaa cagcagacca gttggccgct
     4801 ggtgctggaa catagcgatg tcgtggtgct gtggagtgct aacccactca atacgctgaa
     4861 aattgcgtgg aatgcatccg atgagcaggg gctttcttac ttttctgcac tgcgtgacag
     4921 cgggaaaaag ctgatctgca ttgatccaat gcgatcggaa accgtcgatt tctttggcga
     4981 taaaatgggg tgggtggcac cgcacatggg cactgatgtt gcgctgatgc tggggatcgc
     5041 ccatacgctg gtggaaaatg gttggcacga cgaagcgttt ctggcgcgtt gcaccacagg
     5101 ttatgccgtc ttcgcctctt atttgctggg cgagagtgac ggaatagcga aaaacgccga
     5161 atgggcggca gagatttgtg gtgttggcgc agcgaaaatc cgcgagctgg cggctatttt
     5221 ccaccaaaat accaccatac tgatggcagg ctggggaatg cagcgccaac agtttggtga
     5281 gcaaaaacac tggatgatcg tcacgttggc agcaatgttg gggcaaatcg gcacacccgg
     5341 cggcggtttt ggtctttctt accattttgc caatggtggt aaccccacgc ggcgttctgc
     5401 ggtgctctct tccatgcagg gcagcttgcc gggtggctgc gatgcggtag ataaaatccc
     5461 tgtggcccgc attgttgaag cactggaaaa acctggtggc gcatatcaac acaacggtat
     5521 ggaccgacat tttccggata ttcgttttat ctggtgggcg ggcggtgcca actttactca
     5581 tcatcaggat accaaccgcc tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc
     5641 cgaatgtttc tggacggcgg cggcgaaaca cgcggatatc gttctgcctg cgactacctc
     5701 ttttgagcgt aatgatctca ccatgaccgg tgattacagt aatcagcatc tggtgccgat
     5761 gaagcaagtg gtgccgccac gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt
     5821 aagtgaacgc tgggagaagg gcggctatgc gcggtttacg gaaggaaaaa gtgagctgca
     5881 atggctggaa atgttttata acgttgcccg acagcgcggg gcaagccagc aggttgaatt
     5941 gccgccattt gctgagttct ggcaagccaa tcagttaatt gagatgccgg aaaacccgga
     6001 cagcgagcgg tttattcgct tcgctgattt ttgccgcgat ccgcaggcgc atccattaaa
     6061 aaccgccagc ggtaagattg aaatcttctc gcagcgtatt gccgattacg cttatccgga
     6121 ttgcccgggg catccaatgt ggctggagcc ggatgaatgg cagggtaatg ccgaaccgga
     6181 acagttacag gtactttctg ctcatccggc gcatcgtctg cacagccagc tgaattacag
     6241 ttctctgcgc gaattgtacg cggtagcaaa ccgtgagcct gtgaccattc atcctgacga
     6301 tgctcaggcg cgcggcatac aagatggcga tactgttcgg ctgtggaact cacgcgggca
     6361 aattcttgcc ggagcggtca ttagcgaggg aattaaacct ggcgtgattt gcattcacga
     6421 aggggcatgg ccggatctgg atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa
     6481 cgtgctgacc aaagatctcc ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc
     6541 gctggcatgg ctagaaaaat acaacggtcc ggaactgaca cttacagcgt ttgaaccacc
     6601 ggccagctca taatccaggt gggtagttgg gtttcatcct gccatgcgca atcgacaatg
     6661 tgaaaaccct gcgcctggta aaaatctatc gccggttgat ttttttgata aacctccagc
     6721 atcagatggg gatggcgctg ttgcacatac tgcatcagcg ccttaccaat accgcgcctg
     6781 acggccttcg gtgcgacaaa catcgctgcc agaaatcggc cttccataat gctgacaaaa
     6841 ccaagaagct tactgtcttc ttcccagacc cagttttgtg cgttggcaag ataggcattc
     6901 cgcaccagcg gaatgcactc gcgccagtaa ttcgctttta taaagggatg gccccaggtt
     6961 gtactttcca gccacagttc gaggatcgcg gggagttctg aacgttgcgc ttccctaatc
     7021 atggtttatt tcccggatag caacagcagc caaccacatg atcattcacc agcccacatg
     7081 cctgcataaa ggagtaacag attgtggtac cgacaaactt aaaaccacgt tttttcagtg
     7141 ccttagatag ggcgtcggag gcgggcgtag atgtggggat ttcgctcaac gttgtggctt
     7201 gtgtcacctg tggctgatga tttacaaacg accagacaaa gtcggcaaac ggttcgccgt
     7261 tctgttccat ttgcaggtac gcccgcgcat taccaataat tgcctgaatt ttcccgcgat
     7321 ggcggataat cccggcgtcc tgtaccagtc tttcgatatc ctcttcctgc attgcggcaa
     7381 ccttcaccgg atcgaactga tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg
     7441 tgatccacga taacccagcc tgctgccctt ccaggcagat catttcgaac agttttttac
     7501 tgtcagtttc aggcacgccc cactcattat catggtaggc aatataaagc gggtcctgac
     7561 tcacccagcc gcaacgttcc atactttccc tcgctatgta cccgatcgta aaaatttcag
     7621 ccgcttttct tgacgcatcg gtgaaaaagt caatatttaa tacagggcta ctatgcccga
     7681 tatccttcat acaatgacaa taatattgcc gaattcggct cttccctgga gtcgtgttaa
     7741 tgatcataaa aaaaagcggt ggtcgctggc agctaagcct gctggcgagt gtggtaatca
     7801 gtgccttttt tctcaacaca gcttacgcct ggcaacaaga atatatcgtt gatacgcaac
     7861 ccgggcattc cacagagcgt tacacctggg atagtgatca tcaacctgat tacaacgata
     7921 ttttgtcgca acgtattcaa agtagccaaa gggcgctggg actggaagtc aatctggcgg
     7981 aagaaacccc tgtggatgtg accagcagta tgagtatggg ctggaatttt cctttatatg
     8041 aactggttac aactggcccg gtcgcggcat tacattacga tggcacaacc acctcgatgt
     8101 ataacgagtt tggcgacagt actaccacgc tgaccgatcc gttatggcat gccagcgtga
     8161 gtaccttagg ctggcgtgtt gactcccggc ttggcgatct ccgaccctgg gcgcaaatca
     8221 gctataacca gcaatttggc gagaatatct ggaaggcgca atcaggcctg agccggatga
     8281 cggcgacaaa ccagaacggc aactggctgg atgtcaccgt aggcgctgat atgttgctca
     8341 atcaaaatat tgccgcctat gccgcgctat ctcaggcaga aaataccact aacaatagcg
     8401 attatctgta tacgatgggg gttagtgcca gattttaacg taacaatcac aattgaaacc
     8461 attgaataac aatagttgtg gcgatagtgg gtgctaactt accaaataat aaatttggtg
     8521 aataattgtc gcgtcattca ttcctgaact aaggcatttc attccgttct gatggcattt
     8581 catgccgttt ttccccaggc ataaagtgca cttcgttatg gttgtcggca gagatttttc
     8641 ctttttatta ctgcaggaat actgccatga caccttcaaa ttatcagcgt acccgctggt
     8701 tgacactcat cggtactatc attacccagt ttgcgctggg gtcggtttat acctggagcc
     8761 tgtttaacgg cgcgctttcc gccaagctgg atgcgccggt aagccaggtc gctttctctt
     8821 tcggcttgtt aagtctgggg ctggcaatat cgtcttctgt tgcgggcaaa ttgcaggaac
     8881 gttttggtgt taaacgcgtc accatcgctt ccggcatttt gctgggatta gggttcttcc
     8941 tgacagcgca ttctgacaac ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc
     9001 tggcggatgg cgcgggttac ctgctgacgc tctctaactg cgtgaaatgg ttcccggagc
     9061 gtaaaggtct gatctccgcg ttcgctatcg gttcttatgg tctgggtagc ctgggtttca
     9121 aatttatcga cacgcagctg ctggaaacgg tcggtctgga aaaaaccttt gtgatttggg
     9181 gagcgattgc gctggtgatg attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac
     9241 aggaagtgaa aaccagcaat ggtgtggtgg agaaagatta cacgctggca gagtcgatgc
     9301 gtaaaccgca gtactggatg ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt
     9361 acgtgattgg ggtagcgaaa gatatcgccc aaagtctggc acaccttgat gtggtttctg
     9421 cagccaatgc agtcactgtt atttccatcg ccaacctttc aggtcgtctg gtgctgggca
     9481 ttctgtctga caaaatcgcc cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg
     9541 tgggtatggc ggccctgctg tttgcaccat tgaatgcagt gacgttcttt gcagcgattg
     9601 cctgtgtggc atttaacttt ggcggcacta ttaccgtctt tccgtcactg gtcagtgagt
     9661 tcttcggcct caataacttg gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg
     9721 gtagcatttg tggttcgatt atcgcctcac tgtttggcgg cttctatgtg acgttctacg
     9781 tcatctttgc tctgctgatt ctgtcattgg cgctttctac gacgattcgt cagccagagc
     9841 agaaagtatt gcgtgaggcg catatttaat gagatggcgt tggttattaa cgaaggggcg
     9901 agagagcccc tttttttcgt tta
//
LOCUS       DAPGWD010000152         9921 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPGWD010000152 DAPGWD010000000
VERSION     DAPGWD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.153"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            99..671
                     /locus_tag="RHX88_003642"
     CDS             99..671
                     /locus_tag="RHX88_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HDV2232289.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            719..1450
                     /locus_tag="RHX88_003643"
     CDS             719..1450
                     /locus_tag="RHX88_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV2232290.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1475..3997
                     /locus_tag="RHX88_003644"
     CDS             1475..3997
                     /locus_tag="RHX88_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2232291.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            4008..5081
                     /locus_tag="RHX88_003645"
     CDS             4008..5081
                     /locus_tag="RHX88_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2232292.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPSPGQFTATATITVEIVR"
     gene            5329..6369
                     /gene="pstS"
                     /locus_tag="RHX88_003646"
     CDS             5329..6369
                     /gene="pstS"
                     /locus_tag="RHX88_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDV2232293.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6456..7415
                     /gene="pstC"
                     /locus_tag="RHX88_003647"
     CDS             6456..7415
                     /gene="pstC"
                     /locus_tag="RHX88_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDV2232294.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7415..8305
                     /gene="pstA"
                     /locus_tag="RHX88_003648"
     CDS             7415..8305
                     /gene="pstA"
                     /locus_tag="RHX88_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDV2232295.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8396..9169
                     /gene="pstB"
                     /locus_tag="RHX88_003649"
     CDS             8396..9169
                     /gene="pstB"
                     /locus_tag="RHX88_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDV2232296.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9184..9909
                     /gene="phoU"
                     /locus_tag="RHX88_003650"
     CDS             9184..9909
                     /gene="phoU"
                     /locus_tag="RHX88_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDV2232297.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         2550 a         2277 c         2526 g         2568 t
ORIGIN      
        1 gccgtctctt tggtgtacta ttcctttgaa tcaaaaatac acttattaat ataagtgatg
       61 attcatggta aaggatatat tatatcaatg gagtttttat gaagcgtaat attataggcg
      121 gtgcattcac tctggcatct ctaatgctgg ccgggcatgc actggcagaa gatggtgttg
      181 ttcacttcgt cggtgaaatt gtcgacacta cttgtgaagt tacctccgat acagccgatc
      241 aaattgtccc actgggtaaa gtcagtaaaa atgcattttc aggtgtaggt agtctggcgt
      301 cgccacagaa gttcagtatt aagcttgaaa attgcccggc aacgtacact caagcagccg
      361 ttcgttttga tggtacagaa gcgcctggcg gcgacggcga cctgaaagtg ggtacgccgc
      421 ttacagcagg caaccctggt gattttaccg gtacaggaca agcgattgcg gcaaccggcg
      481 ttggtattcg tatttttaac cagtccgata attcgcaggt taaactttat aacgactctg
      541 cttataccgc tatcgatgct gaaggcaagg ctgaaatgaa gtttattgca cgctatgtgg
      601 caaccaatgc gaccgtaacg gctggtacgg cgaacgctga ttcacaattt actgtcgaat
      661 ataagaaata atttatcaag ggccttttta ttcagttggc tttggccagg gagtcgttat
      721 gtccagaaaa agttgtttaa tcaggctctt taaaacaaca gtttcatgta agttatttac
      781 cgcgattata ctcagcgttt ttttaggcca accagcatta acttatgcag gcgttgtcat
      841 tggtggtacg cgggtggttt acttaagtaa taatgctgat aaatctattt ctgtatttag
      901 caaagaagaa aaaatacctt atctcatcca ggcttgggta gatccattta ataaagagga
      961 taaaagcaaa gcgccgttta ccgttattcc gccagtatca cgattagaac cttcgcaaga
     1021 aaaaatatta cgcattattc acaccaaagg tgtgtcattg cctgatgatc gcgagtcagt
     1081 cttctggctg aatattaaaa atattccgcc atcagcgagc aacaaagcga cgaactcgct
     1141 ggaaatagca gtaaaaacgc gtatcaagct gttctggcga ccagcaaata tccgccttat
     1201 tcccgaagat gctgcgccga aagtgaaatg gcgccgggaa gggcgaaatc tgatagcaga
     1261 gaatcccaac ccaattcata tcagtgtgat ggatgtcatc gttgatggcc atgatgtgcc
     1321 cctcaatatg atccgtcctt ttgaaacgct cacgttacct ttacctgcta attctgcggg
     1381 aggccaaatg acctggcgat ttattaatga ttacggcgca gtcagtgacc cgataaaaat
     1441 gacgctgtaa gcaagggata aggataggcg tattatgatg acgaccagaa tagtggttgg
     1501 cctcacggca gggacgtgtc tgattttctc gcaaaacctg atggccgagg tcagtgtatt
     1561 caatccggcg cttctggaaa tcgaccatca atccggagtc gatattcgcc agtttaatcg
     1621 ggcaaacctg atgcccccag gtgtttatag cgttgatatt tttatcaacg gtaaaatgtt
     1681 tgaacgtcag gatgtgacat ttgttcagga taatccagat gctgatctgc acgcttgctt
     1741 tattgccatt aaaaaaacac tgtcctcctt tggcataaaa gttgatgcgc tcaaatcgtt
     1801 caatgatgtg gatgagacgg tttgcctcga tcctgctcca cgtattgaag gctcatcctg
     1861 gcagtttgac agtgataaat tgcagctgaa tatatccatt ccccaaatct acatggacgc
     1921 gatggcttat gattacatca gccccacgcg ttgggatgag gggattaacg cgctcaccat
     1981 caactacgat ttttctggtt cacatacact acgttcagat tatggttcac aagagacaga
     2041 taccagttat ctcaatctgc gcaatggact gaatattgga ccgtggcggc tacgtaatta
     2101 cagtacttta aacaccagcg atggccgtgc ggaatacaac tccattagta cctggataca
     2161 gcgcgatatt gccgcgttaa gaagccagat tatgattggt gatacgtgga cggcgagcga
     2221 tattttcgac agtacgcaaa ttcgcggcgc gcgtttgtat actgataacg atatgctacc
     2281 cgccagccag aatggctttg ctcctgtggt tcgtgggatt gcaaagtcca acgccaccgt
     2341 catcattcgg cagaatggct acgtgattta tcagtctgcc gttccacaag gtgcttttga
     2401 gatcaccgat ctcaacaccg ccagtacagg tggcgatttg gacgtaacca tcaaagaaga
     2461 agacggtagc gaacaacgat tcacccaacc ttatgcttca ttggcgattc ttaaacgtga
     2521 aggtcagaca gatgttgatg ttagcgtggg tgaattgcgc gatgaagacg gatttacacc
     2581 ggacgtcctt caggcgcaaa tacttcatgg tttttcccac gggatcactt tatatggagg
     2641 tatgcaggct gctgaaaatt atggttctgc agctctgggt gtcggtaaag atcttggcgc
     2701 tttgggcgca atttctttcg atgtgacaca tgctcgtgcg aattttagcc atgatgatac
     2761 agaaacgggt cagtcatatc gctttctcta ttcaaaacga tttgacgaca cagacactag
     2821 cttgcgcctg gttggctatc gttactccac cgagggctac tataccctca atgagtgggc
     2881 atcgcggcgc aacagccctg aagacttttg ggaaacaggt aaccgacgta gtcgcgtgga
     2941 gggaacgcta acgcagtcgt tggggagaga ttatggcaat ttatacctga cattaagccg
     3001 gcaacaatac tggcataccg atgatgtcga acgattaatg caatttggct acagcagtag
     3061 ctggaagcgt ctctcgtgga acgtctcctg gagttattcc aatactgcca gacaggggac
     3121 ggggaacaac catgccagtg ataacaccag tgagcagatc tacatgctct ctttatctgt
     3181 tcctttatcg ggctggtggg gtaatagtta cgccacctat tctgtttcgc aaaacgataa
     3241 ttccggtagc tcacatcaac tcggactcag cggtacggcg ctggaaagaa ataacctttc
     3301 atggaattta atgcagtcct ataacagtca tgatgatgag gttggcggta atatgtccct
     3361 gacctatgat ggctcttatg gcacggtgaa cggcagctat aactacagcc aaaattccca
     3421 gaggctgaat tatggtatca gagggggaat tctggcacac agcgaagggg taacgttaag
     3481 tcaggagtta ggtgaaacta ttgctcttgt taaagcacct ggggccgccg ggttagaaat
     3541 agataatatg cgcggtgctg cgacggactg gcgcggctat acggtcaaga cacagctaaa
     3601 cccttatgat gaaaatcggg tagcaatcag cgataactat ttctcgaagt cgaatataga
     3661 acttgataat accgtcgtta cgatggttcc cacgcgtggt gcagtggtta aagcggagtt
     3721 tgtgactcat gtgggttatc gcgttctctt cagagtgtta aatgcaaatg gtaaaccggt
     3781 accttttgga gccattgctg cgatacaaga tgcaagtttg gcagattcag gaattgtcgg
     3841 tgaccgtggc gaactttatc tttctggtct accagaaaaa ggacaggtta cgttatcctg
     3901 gggagaaaac gcctcaacaa aatgcatctt caattattca ctttcgacac cagaaagtga
     3961 gagcggatta attgaacagg gtgtgacatg tcattaaaag gatttttatg aacaagtata
     4021 tcaaacagtg gtgctttgct gtgtttatgc tctcgttaag tagtgtagca cttgcagctc
     4081 ctaaaggtat ctgtacccct gataatggcg tatttcacag tacgcttgat ttctcgggat
     4141 atcttattac cgcgaacgaa aataaggttg gaacgacatt taacaccact gtaactaatg
     4201 gatcctcata tcctggacgc tgtcattgtg atactggtaa cgtaggagaa ttcccctata
     4261 tctattatac gtcaaaaata aaccaggcat taacttatgc tggggttcac tctaatatta
     4321 attattatga tcttaatccg aaccttgatg ttgggattgc tatagatatt cttggggttg
     4381 ggtatgtgaa tgcacctttt gaatatcatg ctaacaaccc gtctggtaat acaaagtaca
     4441 attgcaaccg cattgaacct ttaagtatat ctagtggggc taaagcgata gtatattttt
     4501 atatcaagaa gacatttgca ggaaaattga ttattcctga aacaaagata gtgacattgt
     4561 atggaacaat tagccgtgac accccggtgg attactcaca gccgatggct gatgtttata
     4621 ttcggggcga tattacagct ccgcaaagtt gcgaaataaa taatttacag ccagtttatt
     4681 ttgattttaa agaaatacca gctgcagatt tttcatctgt tgttggaagt gcggtaacaa
     4741 cacataaaat tactaaaacg gtcactattg agtgtgaaaa cctggggata ctgaataccg
     4801 atgatatcag tacgtctttt tatgctaccg aacccaatac ggacaattca atggtcgtaa
     4861 cttcaaactc taacgtgggg ataaaaattt acgacaaaaa taataaggaa atcaaagtga
     4921 acggtggtga gttgccaaca gacatgggta aatcaacagt gtatggtgaa aaatcaggta
     4981 gtgtaacttt ttcagctgct cctgcgagcc ttactggagc tcgcccatcc ccggggcaat
     5041 ttactgcaac agcgacgata actgttgaga ttgtgcgcta ataggtctga caacgattgt
     5101 tcatcacatc gttgtcatct cacccatttc tttatcactt ttaaatgcat catgacaaaa
     5161 agatgacatg tgggattttc atcttcacat ttaaaatcag taagttattt ggtaattcct
     5221 ttctgtcata aaaatgtcat attccttaca tataactgtc acctgtttgt cctattttgc
     5281 ttctcgtagc caacaaacaa tgctttatga atcctcccag gagacattat gaaagttatg
     5341 cgtaccaccg tcgcaactgt tgtcgccgcg accttatcga tgagcgcttt ctctgtgttt
     5401 gcagaagcaa gcctgacagg tgcaggtgca accttccctg cgccggtgta tgccaaatgg
     5461 gctgacactt accagaaaga aaccggtaat aaagttaact accagggtat cggttcttcc
     5521 ggtggcgtaa aacagattat cgctaatacc gttgattttg gtgcctctga cgcgccgctg
     5581 tctgacgaaa aactggctca ggaaggtctg ttccagttcc cgaccgtgat tggcggcgtg
     5641 gtgctggcgg ttaacattcc agggctgaag tctggcgaac tggtgctgga tggtaaaacc
     5701 ctcggcgaca tctacctggg caaaatcaag aagtgggatg atgaagccat cgccaaactg
     5761 aatccgggtc tgaaactgcc ttcacaaaac attgctgtag tacgccgcgc agatggctcc
     5821 gggacttcct tcgtcttcac cagctacctg gcgaaagtga acgaagagtg gaaaaacaac
     5881 gttggtactg gctctaccgt aaaatggccg atcggtctgg gcggtaaagg taacgacggt
     5941 atcgccgcgt tcgttcagcg tctgccgggt gcaattggtt atgttgaata tgcttacgcg
     6001 aagcagaaca acctggcgta caccaaactg atctccgctg atggtaaacc ggttagtccg
     6061 accgaagaaa acttcgctaa tgcagcaaaa ggtgcagact ggagcaaaac cttcgctcag
     6121 gatctgacca accagaaagg cgaagatgca tggcctatta cctctaccac gttcattctg
     6181 atccacaaag atcagaagaa accagaacaa ggcacagaag tgctgaaatt cttcgactgg
     6241 gcgtacaaaa ccggggctaa acaggcgaac gacctggatt acgccagcct gccggatagt
     6301 gtagttgaac aggttcgcgc tgcgtggaag accaatatta aagacagtag cggtaagccg
     6361 ctgtactaat aaaactccag gccgggtacg gtgttttacg ccgcatccgg cattacaaaa
     6421 tgactttgta aacgcgttta actgaagagt aacttatggc tgcaaccaag cctgctttta
     6481 acccaccggg taaaaagggc gacataattt tcagcgtgct ggtaaaactg gcggcgctga
     6541 ttgtgctatt gatgttgggt ggcattattg tctctctgat catctcctcc tggccgagca
     6601 ttcagaaatt tggtctggct ttcctatgga ccaaagagtg ggatgcaccg aacgatatct
     6661 acggggcgct ggtgccgatc tacggtacgt tggtgacttc gtttatcgcg ctgctgatcg
     6721 ccgtcccggt gagtttcggt atcgccctgt tcctgactga gcttgcgcct ggctggctga
     6781 aacgcccgct gggtatcgcc attgagctgc tggcagccat tccaagtatc gtttacggca
     6841 tgtggggcct gtttatcttt gcgccgctgt tcgccgttta ctttcaggag ccggtcggca
     6901 atatcatgtc gaatatcccg attgttggcg cgctgttctc tggccccgca tttggtatcg
     6961 gtatcctcgc ggcaggcgtg atcctcgcca tcatgattat tccgtacatt gcggcggtaa
     7021 tgcgtgatgt gttcgaacaa accccggtga tgatgaaaga gtcggcctac ggtattggct
     7081 gcaccacctg ggaagttatc tggcgtatcg ttcttccgtt caccaaaaat ggtgttatcg
     7141 gcggcatcat gctggggctg ggccgcgcgc tcggtgaaac catggcggtg acctttatca
     7201 tcggtaacac ctaccagctc gacagcgcct cgctgtatat gccgggcaac agtatcacct
     7261 ctgcgctggc gaacgaattt gcggaagcgg aatccggtct gcacgttgcc gcactgatgg
     7321 aactgggcct gatcctgttt gtgattacct tcatcgtcct cgccgcatcg aagtttatga
     7381 ttatgcgcct ggctaagaat gagggggcac gctaatggct atggttgaaa tgcaaaccac
     7441 tgcggcgctg gctgaatctc gccgcaaaat gcaggcgcgt cgccgcctca aaaaccgtat
     7501 tgcgctgacg ctctcgatgg cgacgatggc cttcggcctg ttctggctga tctggatttt
     7561 aatgtccacc atcactcgcg gtatcgacgg tatgtcgctg gcgctgttca ctgaaatgac
     7621 gccgccgccc aatacggaag gtggtggtct ggcgaacgcc ctggcgggta gcgggctgtt
     7681 aattttgtgg gccacggtat tcggtacgcc gctgggcatt atggcgggga tttatctggc
     7741 ggaatatggt cgtaaatcct ggctggcaga agtgattcgc ttcattaacg acattctgct
     7801 ctccgcgccg tcgattgtgg ttggtctgtt tgtctacacc atcgtggtgg cacagatgga
     7861 gcacttctcc ggctgggcgg gcgtaattgc gctggcgctg ttgcaggtgc cgatcgttat
     7921 ccgcaccacc gagaacatgc tgaaactggt gccggacagc ctgcgtgaag cggcttatgc
     7981 gctgggtaca ccgaagtgga agatgatctc cgcgattacg ctgaaagcgt cggtgtccgg
     8041 gatcatgacc ggtatcctgc tggcgattgc ccgtattgcc ggtgaaaccg cgccgctgct
     8101 gtttaccgcg ctctccaacc agttctggag cacggacatg atgcagccga tcgccaacct
     8161 gccggtgacg atctttaagt ttgcgatgag cccgtttgca gaatggcagc aactggcctg
     8221 ggccggggtg ttgattatta ccctgtgcgt actgctgctg aacattctgg cgcgcgttgt
     8281 ttttgcgaag aataaacacg gttaacactt tgccggatgc ggcgtgaacg ccttatccgg
     8341 cctacggtaa gcctgattcg cgaagtgcat caggcacgat gaggaaaaga ttgcaatgag
     8401 tatggttgaa actgccccga gtaaaattca ggttcgtaat ttgaacttct actacggcaa
     8461 attccatgcc ctgaaaaaca tcaacctgga tatcgctaaa aaccaggtta cggcgtttat
     8521 cgggccgtcc ggctgcggta aatcgacgct gctgcgtacc ttcaacaaaa tgtttgaact
     8581 gtacccggag cagcgtgcgg aaggtgaaat tctgcttgat ggcgacaaca tcctgaccaa
     8641 ctctcaggat atcgccctgc tgcgtgcgaa agtgggcatg gtgttccaga aaccgacgcc
     8701 gttcccgatg tccatctacg acaacatcgc ctttggcgtt cgtctgtttg agaagctctc
     8761 ccgtgccgac atggatgagc gcgtgcagtg ggcattgacc aaagccgcat tgtggaacga
     8821 aaccaaagat aaattgcacc agagcggtta ctctctctct ggtggtcagc aacagcgtct
     8881 gtgtattgcg cgtggtatcg ccattcgccc ggaagtgctg ctgctcgacg aaccgtgttc
     8941 ggcgctcgac cctatctcta ccgggcgtat tgaagagctg atcaccgaac tgaagcagga
     9001 ttacaccgtg gtgatcgtca cccacaacat gcagcaggct gcgcgttgtt ccgaccacac
     9061 ggcgtttatg tacctgggcg aattgattga gttcagcaac acggacgatc tgttcaccaa
     9121 gccagcgaag aaacaaacag aagactacat caccggtcgt tacggttgat tcaggagtgc
     9181 gttatggaca gtctcaatct taataaacat atttccggcc agttcaacgc cgaactggaa
     9241 agtatccgca cgcaggtgat gaccatgggc ggcatggtgg agcagcagct ttctgatgca
     9301 atcaccgcga tgcataacca ggacagcgat ctggcgaagc gcgtgatcga aggcgacaag
     9361 aacgtcaaca tgatggaagt ggcgatcgat gaagcctgcg tgcgcattat cgccaaacgt
     9421 cagccgacgg cgagcgacct gcgactggtt atggtgatca gtaaaaccat tgccgagctg
     9481 gagcgtattg gcgacgtggc ggacaaaatc tgccgtactg cgctggagaa attctcccag
     9541 cagcatcagc cgttgctggt aagtctggag tcgctgggcc gtcataccat ccagatgctg
     9601 cacgacgtgc tggacgcgtt cgcgcggatg gacattgacg aagcggtacg tatttatcgt
     9661 gaagataaaa aagtcgatca ggaatacgaa ggtattgttc gtcaactgat gacctacatg
     9721 atggaagatt cgcgtaccat tccgagcgta cttactgcgc tgttctgcgc gcgttctatc
     9781 gaacgtattg gcgaccgctg ccagaatatt tgtgagttta tcttctacta cgtgaagggg
     9841 caggatttcc gtcatgtagg cggtgatgag ctggataaac tgctggcggg aaaagatagc
     9901 gacaaataat tcaccagaca a
//
LOCUS       DAPGWD010000153         9907 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPGWD010000153 DAPGWD010000000
VERSION     DAPGWD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.154"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..144)
                     /locus_tag="RHX88_003651"
     CDS             complement(<1..144)
                     /locus_tag="RHX88_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232298.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFS"
     gene            222..1316
                     /gene="ddlA"
                     /locus_tag="RHX88_003652"
     CDS             222..1316
                     /gene="ddlA"
                     /locus_tag="RHX88_003652"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV2232299.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1340..1552)
                     /locus_tag="RHX88_003653"
     CDS             complement(1340..1552)
                     /locus_tag="RHX88_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDV2232300.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1812..2120
                     /locus_tag="RHX88_003654"
     CDS             1812..2120
                     /locus_tag="RHX88_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDV2232301.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="RHX88_003655"
     CDS             complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="RHX88_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDV2232302.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="RHX88_003656"
     CDS             complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="RHX88_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDV2232303.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4858..6015
                     /gene="ampH"
                     /locus_tag="RHX88_003657"
     CDS             4858..6015
                     /gene="ampH"
                     /locus_tag="RHX88_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDV2232304.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6016..6639)
                     /gene="iprA"
                     /locus_tag="RHX88_003658"
     CDS             complement(6016..6639)
                     /gene="iprA"
                     /locus_tag="RHX88_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDV2232305.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6727..9729)
                     /gene="ehaB"
                     /locus_tag="RHX88_003659"
     CDS             complement(6727..9729)
                     /gene="ehaB"
                     /locus_tag="RHX88_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HDV2232306.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTI
                     ASWDDVALAVVAHPNADNAMQTTADFSNSTLMGDVIFSSNFDENFFPRGADSYRDADG
                     EVDTNGWDGTDRLDLTLNNGSKWVGAAQSVHQTGSIDVDGDGKGDIATYGVGTEATAT
                     LIDIEDNSLWPLSTVGVENDDTSYSEFDHITGNQVYQSGLFNVTLNTGSQWDTTKTSL
                     IDTLSINSGSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEV
                     SAGWAVGDAALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNI
                     ESNRFVLDADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEV
                     DNNGDHVAAATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAY
                     TYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRVTNSRHGLADNG
                     GAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDR
                     SGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWG
                     FGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAG
                     YTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNF
                     SAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2548 a         2458 c         2356 g         2545 t
ORIGIN      
        1 agaaaaaaac tcaccgcatg gccatttaag ccaggaataa attagcttcc cgttcatcat
       61 ttttgtgatc ttgcgcacat ctttgagata acgcactttc ttcaccagta cgttgcacgc
      121 tttgtcagca gaagattgca acaatctttc agaaggccgc gaatcccccg ttttgtcgtg
      181 gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa ctgcgggtag
      241 gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca
      301 ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt gataaacaag
      361 ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat cctgcccata
      421 ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc aggtaaacat gagcatcagc
      481 ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc ccgattgtcc
      541 acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc aatttaccgt
      601 ttgtaggttc tgatgttctg gcttcagcag cctgtatgga taaagatgtc accaaacgtc
      661 tgctacgcga tgccgggctg aacattgcgc catttattac cctgacgcgc gctaatcgtc
      721 acaacatcag ttttgccgaa gtggagtcta aactggggtt accactgttt gtaaaaccgg
      781 ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag tacgcaattg
      841 ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg atcaaaggtc
      901 gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc tgtggcgaga
      961 tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa gatggcgcga
     1021 aagtggtagt tccggcagcc attgcaccag agatcaacga taagatccgg gcgattgccg
     1081 ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc
     1141 cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc aacatcagta
     1201 tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc acacgtttga
     1261 ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca atgtaatatt
     1321 ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga cgacgaataa
     1381 tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca taggtctgcc
     1441 aaccttttgc ttcaaatccc agtagcccca ccacgccatt aagaatgaat ataaggccaa
     1501 tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc atggcagctc
     1561 cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt gtagacaaca
     1621 tttttgtaat tgttaataaa tttaacttaa ggttgccatt ttgtgactca caacgaagat
     1681 tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg acaatctaaa
     1741 tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc agatgacttc
     1801 cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga aaatatcgca
     1861 atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt tgcttttggg
     1921 ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag cgtttaatgc
     1981 aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc accattttcg
     2041 ggctgatccc gtttttagta ggctgcctca tttttgcagt ggtggcgcta tggctgcact
     2101 ggcgacatcg ccgccagtaa gatttacgtt gccagatacg gcctaaaacg ccttatctgg
     2161 catgacattc ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt
     2221 cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt gaatgccagg
     2281 caacatttcg cggggtagtg atttacccgt tttcgcttcg gcaagttgat aaactttctt
     2341 gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctaactgac ggcgaatttc
     2401 gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt gccagttcgg ttttcccggg
     2461 ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt
     2521 actgactgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt taaaatcagc
     2581 aaagcggtat atcggtgcgc tatagctggc gggataattc agtaaatggt aagtaccaaa
     2641 ccacaacccg ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca ttttccacgg
     2701 ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc caccggtgcg
     2761 caccggatta agcgaaccaa acagcgtctg ccccattggt accatgctga tcagatcgtc
     2821 aaaaatcgcg ctcaattgct tttctgtacg caccgaatct aaccgttcgc tataactttt
     2881 gccgtttggc gatttgattt ttagcgcggt atgaaccaga aaagcaggaa tgtgcatccg
     2941 ttcagcacga cggtcaatct cttgccaggc gatcttgctt aagcccggaa cggccggatc
     3001 tgcctgataa tttgactcct gctgcgccac cgccagcacc gagcagacgt tttccagcgt
     3061 cggtgccagc ccctgacttt caaaggtggt ggcgagatct ttcgcccagg catcgcggtc
     3121 tttcacgctc gcaggcatct tctgccgcac gacgctcgct acatctatcg ctttttcgcc
     3181 ctttttcaac ggttgcggag cctgactact acaccccgct aaaaccagaa ccgccaacaa
     3241 tgacagcgaa gatagatgat tgacgcgcga cattacttct cctttttagc tcaaggtatg
     3301 ggttacttcc tgaattttgt caccatccag ctcatgttca aagctgcgca gacgtttgta
     3361 gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga aagcaccgcg
     3421 aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg taccggcaac
     3481 aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat agagaatgcg
     3541 ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa tagtttttcc gtacggcgct
     3601 aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca tcgtcttcac cataaaccag
     3661 ctctttacgg taggcggcct ctactcgctg gtttttaaac tccagccccg gcagtttgat
     3721 ccctaccact gccagcaatc cggtccccat cagcgaccag acgatagcgg caatcaccag
     3781 accatacgga atgtgcccga caattggcag ctccggcaca tgtgcggaga gcgttaccag
     3841 caccggcagg aaggcgatca acgtcatgat ggcgttgata aaactgacgc ccatattctc
     3901 cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggccc cttcgatatg
     3961 acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc agcggaacac
     4021 gtagtgactg acaaagaagt tgttcagtac actgatcacc accgcgatca gcgcaatccc
     4081 cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg gcgaacttag
     4141 cgcggtttga atcagatcat agaacggtgc ataccaggcg ttgacggcga ccccgacttc
     4201 caccaaaaac caggtgacga agatgatcag tgcagtaccg agtattgacc agtattgcca
     4261 acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa caatgtagta
     4321 agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc
     4381 ggaagcgccg gtgatacgcg ccacccagtc acccccaccg gcttgccaga agataacggc
     4441 aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc ggctttggga aaaaagactt
     4501 aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta ttaaccgcat
     4561 gaccgctaca aagcgtcagc caaatgaaac cgcccattgt gacccatagc aaggcgctta
     4621 gttcgccagc ctgaccataa gaattgtttc gacggatttc acaaaccaaa gattgtttac
     4681 ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag gaaaaagatc
     4741 gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc tttgcaatcc
     4801 atcaatgagt agtataaata cgctcagtta ccttcattca atctatggac accaccgttg
     4861 aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc cgtccatgcg
     4921 gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta tgccgatcat
     4981 attttttacg gcagcggtgc cacggggatg gcgctggttg ttatcgacgg taaccagcgc
     5041 gtctttcgta gttatggcga aacgcgacct ggtaataacg ttcgcccgca gctggattcg
     5101 gtcgtgcgta ttgcttccct caccaaacta atgaccagtg aaatgctggt gaaattgctc
     5161 gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat atgctccgcc aggcgcacgc
     5221 gtgccgacct acaatgggac gcccattacc ctagtgaatc tggcaaccca taccagcgcc
     5281 ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg gccaacgcgc
     5341 gaacaacgct ggaaatacct ttctacggcg aagctaaaag ccgcgccagg cagtcaggcg
     5401 gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc gtccggcaag
     5461 ccttataccc agttgtttga agagcagatt acccgtccat tggggatgaa agacaccacc
     5521 tacaccccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg
     5581 tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc tggcgatatg
     5641 atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa ccaggcagat
     5701 cgcatgcaga cgttgattta tcagcgcgcg cagtttacga aagtgattgg catggatgtc
     5761 cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa agagggtcgt
     5821 ccggggatta ttcagaagac aggcggtggt ggcggtttca ttacctatat ggcgatgatc
     5881 ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac gcgctttaaa
     5941 aatatgagcg atggcatcaa tgacctggtg accgagctaa gcgggaataa accgttggtt
     6001 atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca gtttgccttt
     6061 attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga cgacagaacg
     6121 cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc gcaattcttc
     6181 attccagtca atcattgata ataatgttgc gcggatttgt tcgtaggaat tatgcccaat
     6241 gagctgcacg tcgcgtaatt ccagaattct attttgccag gctaaccagt aaaaagcgtc
     6301 tcgccagagt tgattttgtt caataagcgt aatggtttgt ttggctggta gatgatatcc
     6361 cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta aaccatcagc
     6421 cagccccata atataaggag cctgggtaat accgataagt acgttttctt ctctacgcag
     6481 agagataacg ccctctaaaa taacgaaagt atcttcgcta ttgacatcac tggaaaatat
     6541 aacttgcttt tcattattaa actcgaagcg cgtaccgtat ctggacaaac atttatcgag
     6601 cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct ttgcagtaat
     6661 gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagaatttc ttcggagtga
     6721 agaatattac caggtatatt taacacccac gttcgcggac cagtcttgat ctacgtcacc
     6781 accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc tgaactgagt
     6841 acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat caccgttcac
     6901 atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc
     6961 ctggtcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat aacgcatgct
     7021 gtcgtaagac tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat caccagactg
     7081 gaacagacca gaaatgctgc cgtaaggagt cacgtaacca gcatcaccca gtttgaagtc
     7141 gtaaccggct ttcaaaccga agccccaagc gtcggagttg gtgctaccgt caacgtaagt
     7201 accgttgctc atggttgcag acaagtcgtt gttgaagtga gagtaactca agctaccatc
     7261 aacaaagacg ttgttcgcga agtgagcaga agagtagatg taggcagtct ggctgtcttg
     7321 atccacctga ccagaacggt cattcatgtc acctttagcg aagcctgcag ccgcaccgac
     7381 gatccactta gcgttgttac cgtcaatttt ggtatcaaca ccgaccatga tgccgttaac
     7441 atcctgatca tagttgatgg tgccgttgtc gccgttgaag ttaccaccga agtagcttac
     7501 ccatgcgccg ccgttatcag ccaggccatg acgagagttg gtcacacgag taccaacggt
     7561 gtcttgttcc aggttccaga tattggtgtt cgcagacggg atgctcagcg ccatgttagc
     7621 gtagtcggtc agctccatct gttgcagaac aacggtgtta ccgcgctgtt cagcctgata
     7681 ggtgtatgca cccaggtcag ctttgttagc agcagagaag gtcgcgttgc tgtttttgtc
     7741 gttgacgtag atcagctcgt tgccgttgta gtcagcgata gaaccagcac cagttgcgtt
     7801 gtcgatacga actttgtagt taccggttgc agctgcaaca tggtcaccgt tgttatcaac
     7861 ttcgttctga ccagcttcga tagaagcagt gttgtcgtta tcgccgttac cgttaatggt
     7921 caggtgacca tcagagttca ttgcgattaa gccataaccg tagtttgact tggtagtatc
     7981 gttggtacgg tcgttggtca ggtctgcatc aagtacgaag cggttgcttt cgatgttgaa
     8041 cacaccagcg tgaatgttac cactggtatc agtagtgctg gtcaggttca gagtatcaac
     8101 ctgcagagca ttcgccgcag tgttacctac atccagaata ccgtcgttag tcactttgat
     8161 gttgttggca tacagagcag catcacctac agcccaacca gcagaaactt catcagaaat
     8221 ggttacggta ctgttgtcga cagtcagtga atcagttgca acatgaccat cttcgttgat
     8281 gttcagcgca gaaagacctg tcagagagat agagtcagaa atcagcgttg aatcagcaac
     8341 attaacagtt gaaccactgt tgatgctcag agtatcaatc agagaagttt tggtggtatc
     8401 ccactgtgaa ccggtattca gggtcacatt gaacagaccg ctctggtaaa cctgattacc
     8461 agtaatatga tcgaattcac tgtaacttgt atcatcgttt tcaacaccaa cggttgataa
     8521 cggccacagg ctattatcct caatatcaat cagagttgca gttgcctcgg tgccaacacc
     8581 gtatgtagcg atgtcacctt taccatctcc atcaacatca atagaaccag tttgatgaac
     8641 agattgtgct gcccctaccc atttactacc gttatttaat gtcagatcca ggcgatctgt
     8701 tccatcccaa ccgttggtgt ctacttcacc atcagcatcg cgataactat ctgcaccacg
     8761 tgggaagaag ttttcatcaa agttgctaga gaagattaca tcgcccatca aagtagagtt
     8821 actaaagtca gcggtggtct gcatagcatt atcagcattc ggatgtgcaa ctacagctaa
     8881 tgcaacatca tcccagcttg caatagtgct atcagcaaca ccatctccgt cagtatctac
     8941 ggtgacaaag ttagatttac cactataatc gctggcatta cctgtattac cgaaccaacc
     9001 agtagtacct tcatcagtcc atgaaccaga ggtcacggtt gaatctttaa cagtgatggt
     9061 gttattaaac acttcgctgc tgtttacgcc agtgctgaca gtgttgtcgt caatatcttc
     9121 ccactcataa ccctgagtca acgtaatacc cgcaacgtga gagttattag tgatggacag
     9181 atcaacttcc tgatccagag taatagccgt accaatgttg taaacgtcta caacatgagt
     9241 atcagcggca ttattgtaag tgccgttata tgtgtagtgt tcgtagttgt cgtcgatcgt
     9301 tgaattatca acagtcagcg ccagacggtc atggtagtaa tcatccgcac gatcagcaca
     9361 atcagttgtc atgcactcgg aataaatcat accgtgaata gtgctgtttt tgattgtcag
     9421 actattggcg ttggtattgg tagaaatacc gtcgtccaga tagtaggtag aaatcacgcc
     9481 gttaacggta gcgttattaa tgaccgggta gatatcgccg ttgtaatagc tatcagcggc
     9541 atagttgttc cagcctacat aaccatcata gtaaacgtca tcagcatagg tggcgtcgtt
     9601 gtagtgatgg aaagtattgt aagtttgacc agaaatatcg ttggtcgctg cattagcctg
     9661 agaggtgata gccagagtgc aagccaatgc taattgtgat actacaagtt tctttttcca
     9721 ggagtgcatt tgtcatccct cctcagggac gtaataagtt gatccatcaa ttatcaatgc
     9781 atagaaaaca tattatgttt cgcgctaacg attatgctgc gttacagacg aatggttcaa
     9841 ttattctttc ccctaagtcc gaatgtggac atatataatt tatggagaaa aacacgcatc
     9901 aaattga
//
LOCUS       DAPGWD010000154         9878 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPGWD010000154 DAPGWD010000000
VERSION     DAPGWD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.155"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            129..344
                     /gene="bdm"
                     /locus_tag="RHX88_003660"
     CDS             129..344
                     /gene="bdm"
                     /locus_tag="RHX88_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDV2232307.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            446..583
                     /gene="sra"
                     /locus_tag="RHX88_003661"
     CDS             446..583
                     /gene="sra"
                     /locus_tag="RHX88_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDV2232308.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            740..2437
                     /gene="maeA"
                     /locus_tag="RHX88_003662"
     CDS             740..2437
                     /gene="maeA"
                     /locus_tag="RHX88_003662"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV2232309.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2571..3581
                     /gene="adhP"
                     /locus_tag="RHX88_003663"
     CDS             2571..3581
                     /gene="adhP"
                     /locus_tag="RHX88_003663"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDV2232310.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3728..4012
                     /locus_tag="RHX88_003664"
     CDS             3728..4012
                     /locus_tag="RHX88_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV2232311.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4063..4716)
                     /gene="fdnI"
                     /locus_tag="RHX88_003665"
     CDS             complement(4063..4716)
                     /gene="fdnI"
                     /locus_tag="RHX88_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDV2232312.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4709..5593)
                     /gene="fdnH"
                     /locus_tag="RHX88_003666"
     CDS             complement(4709..5593)
                     /gene="fdnH"
                     /locus_tag="RHX88_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDV2232313.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5606..8653)
                     /gene="fdnG"
                     /locus_tag="RHX88_003667"
     CDS             complement(5606..8653)
                     /gene="fdnG"
                     /locus_tag="RHX88_003667"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8066..8068),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV2232314.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8885..9766
                     /gene="yddG"
                     /locus_tag="RHX88_003668"
     CDS             8885..9766
                     /gene="yddG"
                     /locus_tag="RHX88_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDV2232315.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2392 a         2601 c         2488 g         2397 t
ORIGIN      
        1 aaaatataaa aatcatcaac caggactaat cttaacaacg aagcggcaaa tatttgccat
       61 gctgaatgtg ctttataaat ctgcgatccg tagcagacac cataaataca cagacacgga
      121 gaatcactat gtttacttat tatcaggcag aaaattcaac agcagaaccc gctctggtta
      181 atgccattga gcaaggcctt cgggcagagc acggtgttgt cactgaagat gacattctca
      241 tggagctgac caaatgggtg gaagcttctg ataacgacat cctcagtgat atctaccagc
      301 aaacgatcaa ttatgtggtc agtggccagc accctacgct ttaaggtgct atgcttgttc
      361 ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa
      421 ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg
      481 actggaccat aaaatttcta accagcgcaa aatagttacc gaaggtgaca aatccagcgt
      541 ggtaaataac ccaaccggca gaaaacgccc cgctgaaaag taattcataa ccatcagtcc
      601 tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt ttgtttttat ctgctttata
      661 cttggggccg acgccctggc ggtaaagcaa agacgataaa agccccccag ggatggatat
      721 tcaaaaaaga gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc
      781 ttacgctggc cctgtactgc tggaatttcc gttgttgaat aaaggcagtg ccttcagcat
      841 ggaagaacgc cgtaacttca acctgctggg gttactgccg gaagtggtcg aaaccatcga
      901 agaacaagcg gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca
      961 catctacctg cgtaacatcc aggacaccaa cgaaaccctc ttctaccgtc tggtaaacaa
     1021 tcatcttgat gagatgatgc ctgttattta taccccaacc gtcggcgcag cctgtgagcg
     1081 tttttctgag atctaccgcc gttcacgcgg cgtgtttatc tcttaccaga accgccacaa
     1141 tatggacgat attctgcaaa acgtaccaaa ccataatatc aaagtgattg tggtgactga
     1201 cggtgaacgt attctggggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg
     1261 taaactgtcg ctctataccg cctgtggcgg catcagcccg gcgtataccc ttccggtggt
     1321 gctggatgtc ggaacgaaca accaacagtt gcttaacgat ccgctgtata tgggctggcg
     1381 taatccgcgt atcactgacg acgaatacta tgaattcgtt gatgaattta tccaggctgt
     1441 gaaacaacgc tggccggacg tgctgttgca gtttgaagac tttgctcaaa aaaatgcgat
     1501 gccgttactt aaccgctatc gcaatgaaat ttgttccttt aacgatgaca ttcagggcac
     1561 tgcggcggta acagtcggca ccctgatcgc agcaagccgc gcggcaggtg gtcagttaag
     1621 cgagaaaaaa atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat
     1681 catcgcccag acccagcgcg aaggattaag cgaggaagcg gcgcggcaga aagtctttat
     1741 ggtcgatcgc tttggcttgc tgaccgacaa gatgccgaac ctgctgcctt tccagaccaa
     1801 actggtgcag aagcgcgaaa acctcagtga ctgggatacc gacagcgatg tgctgtcact
     1861 gctggatgtg gtgcgcaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg
     1921 gctgtttacg gaagagatca tccgtgagat gcataaacac tgtccgcgtc cgatcgtgat
     1981 gccgctgtcc aacccgacgt cacgcgtgga agccacaccg caggacatta tcgcctggac
     2041 tgaaggtaac gcgctggtcg ccactggcag tccgtttaat ccagtggtat ggaaagataa
     2101 aatctaccct atcgcccagt gtaacaacgc ctttattttc ccaggcatcg ggctgggtgt
     2161 tattgcttcc ggcgcgtcac gtatcaccga tgagatgctg atgtcggcaa gtgaaacgct
     2221 tgctcagtat tcgccgctgg tcctgaacgg cgaaggtctg gtactaccgg aactgaaaga
     2281 tattcagaaa gtctcccgcg caattgcgtt tgcggttggc aaaatggcgc agcagcaagg
     2341 cgtggcggtg aaaacctctg ccgaagccct gcaacaggcc attgacgata atttctggca
     2401 agccgaatac cgcgactacc gccgtacctc catctaagcc tgcgcccggt agtgaaggct
     2461 accgggctat ttccctctcc ctttttcaga tctcatccat actgggtagt ggcgaataaa
     2521 tctcatttgc ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaaggctg
     2581 cagttgttac gaaggatcat catgttgacg ttacggataa aacactgcgc tcactgaaac
     2641 atggcgaagc cctgctgaaa atggagtgtt gtggtgtatg tcataccgat cttcatgtta
     2701 agaatggcga ttttggtgac aaaaccggcg taattctggg ccatgaaggc atcggtgtgg
     2761 tggcagaagt tggcccaggt gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt
     2821 ggttctacga aggatgcggt cattgcgaat actgtaatag tggtaacgaa acgctctgcc
     2881 gttcagttaa aaatgccgga tacagcgttg atggcgggat ggcggaagag tgcatcgtgg
     2941 tcgccgatta cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta
     3001 cctgtgcggg ggtcaccacc tacaaagccg ttaaactgtc aaaaattcgt ccaggccagt
     3061 ggattgctat ctacggtctt ggcggtctgg gtaacctcgc tctgcaatac gcgaagaatg
     3121 tctttaacgc gaaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg
     3181 aaatgggtgc agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg
     3241 agaaaaccgg tggcgctcac gctgcggtgg taacagcagt tgctaaagct gcgtttaact
     3301 cggcagttga tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt
     3361 ctatgagcct ggatatccca cgccttgtgc tggatggcat tgaagtggtc ggttcgctgg
     3421 tcggcacgcg ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc
     3481 cgaaagtcgc cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag
     3541 gcaaaatccg tggccgtatg gtgattgatt tccgccgtta agaggccttt gctgcgagtg
     3601 ccagtcctgg tgtcgcagca tcgcactctc tccgacatta aatggctaaa tggattattg
     3661 ttccttccta cgcaacaata atgatagtgt tcctgatgaa ggaacactac tgagagtaaa
     3721 gacactgatg aaaatggcca atcatccccg cccgggggac attattcagg aatcactgga
     3781 cgaacttaat gtcagcctgc gcgagtttgc cagagcaatg gaaattgctc cctcaacggc
     3841 aagccgattg ctgaccggaa aagcagcttt gacaccagaa atggcgatta aactttccgt
     3901 ggtgatcggc agttcgccgc aaatgtggct gaatctgcaa aacgcctgga gtctggcaga
     3961 ggcagaaaaa acggtggatg tgtcgcgact acgccgtctg gttacgcaat aaaaaaatgg
     4021 cgcttcttaa agcgccattt tcagcttaaa gttcaaaaga gattatatcc cttcttcact
     4081 ctcttttttc gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc
     4141 ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc gaccctttca cccagaatgc
     4201 catatacata tggatcagga tggcgtggat caggatgata cccgcagccg cgtggatcag
     4261 caggctgtag cgaacaacct gcatcgggaa gtactgcgca aagtacggac gccagataat
     4321 caccccggtc accagcagca cgaaaatcat gctcatgatc gaccagaaca tcatcttttg
     4381 cccggcgttg tacttaccga catccgccac tttatgctca ttgcctttca atacttcgac
     4441 aatgttcaac agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac
     4501 aaacataaac atcagtgcga cgaaaatcgc aatgccgaaa aacgggtgca aaatgcgtcc
     4561 catctgcggc gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat
     4621 cccggacagc gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat
     4681 aaatttggtg cgcacaatca ttttcgactt actcatgatg atcctcctcg tcatcgtcca
     4741 cttccttatt cgggccaata ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa
     4801 agccagccgc tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct
     4861 tcggatcttt cggcagaccg tgatacagct ccggctgatc ggcgtgatgc agcacgtaca
     4921 taacgtgcgt accaccgacc ccttccgggt tgtagacgcc agcatgttcg taaccacgcg
     4981 ctttcagttt cgccacgcgc tgttccgcca gctccagcat ctccttcttg gtgccgaagt
     5041 ggatagcccc ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat
     5101 cgacgcagag cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa
     5161 acggacaccc ggcaatgcag taaccacagc cgatgcagtt ttccgactgg aaatcgacaa
     5221 tcccgttagc gtactgaatg attgcaccag cagacgggca cgccttcagg cagccgggat
     5281 cttcacagtg catacagccg tctttacgga tcagccactc cagcttgccg ttctgttcgg
     5341 tttcgctaaa gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa
     5401 ccccgacgca gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac
     5461 aggctttaca gccgatacag gtggaaacgt cgataagttt tgcgacttct gctttgtaat
     5521 cacgcacctg agaaggcggc gtgatggagt tagttgcgga ccttttgata atgtcctgcg
     5581 tttccatagc catctgttcg cccccttacg ccttctcgat gttgactaag aacgctttat
     5641 attccggcgt ttgcgagttt gcatcaccga cattcggcgt cagagtgtta gcgatataac
     5701 ctttacgcgc gacaccctca aagccccagt ggattggaat acccaccgtt tcaacctgct
     5761 gaccgtttac attcagcggc ttcagacggc gcgttaccac agccaccgcg cggataaatc
     5821 cacgcttgct tgagacagtg acacgatcgc cattattaat gcctttcgcc gccgccagcg
     5881 tttcgctgat ttccacaaac tgttccggct gagcaattgc gttgagcaat gcgtgcttgg
     5941 tccaggtgtg gaagtgctcg gtcagacgat aggtcgtacc cacatacggg aactgctctt
     6001 ttttacccat ccgcagcgcg tcttgttcat acagacgaac aaccgggtta gacaccacgt
     6061 tcggatgcag cgggttagtg cccagcggcg tttcaatcgg ctcgtagtgt tccgggaacg
     6121 gaccttccgc cattttgttg atggcaaaca ggcgtcccat cccttccggc tgcatgataa
     6181 acggcccggt tggcgtaccc ggtgcggcat tgccgaagtc cggaatatcg ttacccgtcc
     6241 acttgctgcc gttccactgg atcagcatcc gtttcggatc ccacggttta ccgttgatat
     6301 ccgccgaagc acggttgtac agcacgcgac ggttgagcgg ccacgcccag gcccatccca
     6361 gcgtattccc cagaccggac gggtcggagt tatcgcggtt agccatctgg ttgccctgct
     6421 ctgtccagct accggtgtag atccagcaag acgatgcggt ggtaccgtca tcacgcagat
     6481 gcgcaaagct actcagcaac tgacctttct tcgcaatcag cacgccatta gcgtcataga
     6541 gatcttccag cgcatagccg ttgttctctt tagctacttc gtcagattgc ggttcgtgcg
     6601 gctgcttgta gttccagctc atcttcatca gcggttctac gcctttacca ccttcggcct
     6661 ggtacaactc gcgcagatga tggtagatac ccgccagaat ttcgccgtcg ttacgcgctt
     6721 cgcccggcgc gtcctgacct ttccagtgcc actgcaacca gcgaccggag ttagcgatag
     6781 aaccatcctc ttcagcaaag caggtcgaag gcagacggaa tacttcagtc tgaatagacg
     6841 ccggatcgac atcgttcgac tcgccgtggt tctgccagaa ggtagaggtt tcagtcacca
     6901 gcggatcgat aaccaccatg tacttcagct tgctcaggca gctcaccact ttgtttttgt
     6961 ccgggaagga cgcaaccggg ttaaagccct ggcagaaata accggtgact ttgccttcat
     7021 ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc ccacttcggc agccagtcat
     7081 agccccagtt gttctctttc tgcgcggcat cgccatagaa agatttcatc aggctaacga
     7141 agaacttcgg atagttgctc cagtagttca cctgatcagc tagcgtcgct ttcggcgtgt
     7201 tcgcttccag atacgactgc aaatcaacct gtttttctga cggcagcgtc agataacctg
     7261 gcaggctggt agagagcagg cctaagtcag tcaagccctg aatgttggag tgaccacgca
     7321 atgcgttcac gccgccaccg gccataccca tgttaccgag cagcaactgg atcatcgcca
     7381 tagtacggat gttctgcgca cccacagtgt gctgcgtcca gcccagcgcg tacaggaagg
     7441 tggttgtgcg atccggtgcg ctggtggagg ccagcacttc acacactttc aggaagtcgg
     7501 cttttggcgt accacagatg ttttcaacaa cgtccggcgt gtagcgggaa acgtgcgctt
     7561 tcagcaggtt ccacacacag cgcggatgag tcagtgtttc atcgcgtttc gcatagccgt
     7621 tttcatcgaa ctgatagttc caggacgatt tatcgtattg ccgtttttca gcgtcgtagc
     7681 cgctgaacag accgtcttcg aaagcaaaat catcacgcac cagcaggctg gcgttggtgt
     7741 aatgcttaac gtattcggcg ttgattttgt cgttttcgat caggtagcgc aaaacgccag
     7801 acaggaacgt aatgtccgta ccggaacgaa taggtgcgta aatatccgcc acagaagcgg
     7861 tacgcgtaaa acgaggatcg acaacgatca aggttgcatc gttgttgttt ttcgcttcca
     7921 tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt accgcccatt accattacga
     7981 cgttagcgtt tttgatatcc acccagtggt tggtcatcgc accgcgacca aatgttggag
     8041 caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc
     8101 cgagggagcg ggcaaatttc tgtgtcagca tcccggtttc gttgctggca ccggaggcac
     8161 acagcatacc ggtagaaagc caacggttta ccgttacgcc ctgctcgttc ttttcaataa
     8221 agttagcgtc acggtcagct ttcatcagct ttgcaatacg ggagaatgct tcttcccagc
     8281 taatgcgctg ccatttgtca gaacctggcg cacgatattc cgggtagcgc agacggtttt
     8341 cgctgttgac gtaatccagc aaaccggccc cttttgggca cagcgcacca cggcttaccg
     8401 gatgatccgg gtcaccttca atgtgataaa tcgcttctct ggcgtttttc gcgccatcac
     8461 ccaggctata catcaatagc ccgcaaccta cggaacagta tgtgcaggtg ttacggatct
     8521 ctttagcgcg taataatttg tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc
     8581 ccaatgccgc taccgttgtt ccagccatac cgcccgcgca gattttaaaa aattgtctgc
     8641 gactgacgtc cattgctttc ctcttttttc aggggtatta ctgcgcgagg aaactaacag
     8701 taaaattctg aatctttttg cgcgaaatca aaaagaacac gtgtttgcca caaaataatc
     8761 cgcctgcgca aattgaccta cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc
     8821 cagttaaaaa aatgctataa aattcagctt aatttttaac ggcaagagag acaaaacagc
     8881 gagcatgaca cgacaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac
     8941 gatggtagga ttgattcgcg gtgtcagcga ggggctcggc ccggttggcg gcgcagctgc
     9001 tatctattca ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattagaca
     9061 aatcccgaaa ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct
     9121 ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg attgaagtgg gtatggtgaa
     9181 ctatctatgg cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa
     9241 ctggctgatt gtacctggat tattattagc ccttgtcggc gtctgttggg tgttaggcgg
     9301 tgacaatggg ttacattatg atgaaatcat caataatatc accaccagcc cattgagtta
     9361 tttcctggcg tttattggtg cgtttatctg ggcagcctat tgcacagtaa cgaataaata
     9421 cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta acgggagcaa gtctgtgggt
     9481 ttactatttt cttacgccac aaccagaaat ggtatttagc acgcccgtca tgattaaact
     9541 catctctgcg gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatattgca
     9601 tggcaatgtc accataatgg cggtaggctc gtattttacg cctgtacttt cctcagcgct
     9661 tgcagccgtg ctgctcagcg ccccgctgtc gttctcgttc tggcaaggcg cgctaatggt
     9721 ctgcggcggt tccctgctct gctggctggc gacacgtcgt ggttaaaata attgccgggt
     9781 ctacggcccg gcagttaatc agataaattc cgacagtatt aaaaaataaa tttcaaatac
     9841 cagtaattca ccttatatct catttcgact tcgatcac
//
LOCUS       DAPGWD010000155         9851 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPGWD010000155 DAPGWD010000000
VERSION     DAPGWD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.156"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..232)
                     /locus_tag="RHX88_003669"
     CDS             complement(<1..232)
                     /locus_tag="RHX88_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV2232316.1"
                     /translation="MKGNILGDIRAEHDEKMLEASFWQTTDYKALLESYDRCIIVGRR
                     GTGKSALVHMLSKHWHAKPKTYVMTISPVEEQI"
     gene            complement(486..1235)
                     /locus_tag="RHX88_003670"
     CDS             complement(486..1235)
                     /locus_tag="RHX88_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001471407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HDV2232317.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREAVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPMQAELPEETTTATVVKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCIPGDNLPAEPKAASVTETDFKAFPQ
                     PAGVALPVQKDATQEDIKTETVADIVQSLPSFTATREDDLILPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHWDIVHKVIAAGMEK"
     gene            complement(1258..2004)
                     /locus_tag="RHX88_003671"
     CDS             complement(1258..2004)
                     /locus_tag="RHX88_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV2232318.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIAK"
     gene            complement(2011..2799)
                     /locus_tag="RHX88_003672"
     CDS             complement(2011..2799)
                     /locus_tag="RHX88_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232319.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNAECNARGVTKSAIDRITFMAGFADALIQVGWLVETNGVLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTKTDQKALPEEEKEEDINTYLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFSPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            complement(2877..3299)
                     /locus_tag="RHX88_003673"
     CDS             complement(2877..3299)
                     /locus_tag="RHX88_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232320.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETNAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQDALSTAIDAHDDAVQAVYRKAHFSGGGSPSDSVVVH"
     gene            complement(3296..3550)
                     /locus_tag="RHX88_003674"
     CDS             complement(3296..3550)
                     /locus_tag="RHX88_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV2232321.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            3630..4049
                     /locus_tag="RHX88_003675"
     CDS             3630..4049
                     /locus_tag="RHX88_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2232322.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQIRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            4292..4471
                     /locus_tag="RHX88_003676"
     CDS             4292..4471
                     /locus_tag="RHX88_003676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232323.1"
                     /translation="MQQLVPPPGVKGIDKMVHYEVVQYLMDCCGITYNQAVQALRSND
                     WDLWQAEVAIRSNKM"
     gene            4482..4637
                     /locus_tag="RHX88_003677"
     CDS             4482..4637
                     /locus_tag="RHX88_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaF family protein"
                     /protein_id="HDV2232324.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LEKHTVS"
     gene            complement(4634..5122)
                     /locus_tag="RHX88_003678"
     CDS             complement(4634..5122)
                     /locus_tag="RHX88_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion B family protein"
                     /protein_id="HDV2232325.1"
                     /translation="MLDVFTPLLKFFANEPLERLMYTIIIFGLTLWLIPKEFTVAFNA
                     YTEIPWLFQIIVFAFSFVVAISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFL
                     KTGNLIITSPCRNPVMKKLERKGIIQHQSDSANCSYYLVTEKYSHFMKLFWNSRSRRF
                     NR"
     gene            5564..5785
                     /gene="kilR"
                     /locus_tag="RHX88_003679"
     CDS             5564..5785
                     /gene="kilR"
                     /locus_tag="RHX88_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HDV2232326.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            5785..5955
                     /locus_tag="RHX88_003680"
     CDS             5785..5955
                     /locus_tag="RHX88_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232327.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            6030..6305
                     /locus_tag="RHX88_003681"
     CDS             6030..6305
                     /locus_tag="RHX88_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232328.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPTEKKAEFLATLIAMKLKGEI"
     gene            6407..9007
                     /gene="recE"
                     /locus_tag="RHX88_003682"
     CDS             6407..9007
                     /gene="recE"
                     /locus_tag="RHX88_003682"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HDV2232329.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEQDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGISKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEQEEFSNRFIVAPEFNRRTNAGKEEE
                     KAFLMECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETG
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLSDCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            9000..9809
                     /gene="recT"
                     /locus_tag="RHX88_003683"
     CDS             9000..9809
                     /gene="recT"
                     /locus_tag="RHX88_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HDV2232330.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNSDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSLSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
BASE COUNT         2778 a         2335 c         2239 g         2499 t
ORIGIN      
        1 taatttgttc ttcaaccgga ctaatagtca tcacataggt ttttggctta gcatgccaat
       61 gtttggaaag catatgcacc agagcacttt ttcctgttcc tctgcggcca acaatgatgc
      121 atcgatcgta ggattcgaga agagctttat aatcagttgt ttgccaaaat gaagcttcca
      181 acatcttttc gtcatgttca gctcggatat ccccaagaat attacctttc atccagattc
      241 ctttttaacc cattgaggtg acagcacaaa tagtatctta acacctgatg atttcatcag
      301 aacctgataa atagacgtga tataaatatg actaagatca catttcatga aaaaattcga
      361 cgagtttata ttaactgcag cgaatacaaa tcaccgcagt tttcattacc cctatctgaa
      421 gaactctgca caaccgatca tattggtcac aactgcagca ggatgactca tataatcagg
      481 agtatttatt tctccatacc tgctgcaata actttatgga caatatccca gtgcttgttc
      541 agctcccgca gcgcggcgca gactcgctcc cacttctgga catgattctt cgccctacgc
      601 agttcgcggt ttgccatatg cagcgatggc aaaatcaaat catcttctcg cgttgcagta
      661 aacgatggca gcgactgcac aatgtccgct acagtttctg ttttaatatc ttcctgtgtt
      721 gcatctttct gtaccggtaa cgcaacacct gctggctgag gaaaggcttt aaaatcagtt
      781 tccgttaccg atgcagcttt cggctctgct ggtaaattat cgcccggtat gcagtaacga
      841 aatttaccgt tctggtttac gcgaatcagg cgtcctttgc tgattgccat tgccagcgtt
      901 gaagcaactt tgcgggatgt tgtaccgaac agcgtagcca gttcatccgc cgtttgtggt
      961 ccgcgttgtt caatcgtcgc ggttaaatcg cactctgaga ttttcactac agttgccgtg
     1021 gtggtttctt ccggcagttc tgcctgcatt ggctgttcct gctgaacgtt gttatcagcc
     1081 acacgccagg tgtacgcgct tttatcaacg aaaccagctt ttttcagttc ccatagttcg
     1141 ttcagcactg cttcacgact gatatcaagt cgcgcagcaa gttctatgga tgtggctttt
     1201 cccattgctt tcagtgcgtc aaaaacagtc tccattaaat ttttctcccg gtaaaaatta
     1261 cttcgcaatt cctggctgga cgacattcgg acgccagctc tcccagttga aattcaccca
     1321 tcgcccgccg ttcatggtca tgcgatccat aatccgctcg ccaagcaatg ttttcatggc
     1381 ctcatagttc aggtttgtca gcatccccac gctgcgcatc gacgctgtcc ggcgatcaac
     1441 aatctggtgc agtaccacct gctcgttttt cgtctcgcgc tgaatgccaa tttcatcaag
     1501 aaccagcaga tccacttcgc acagttcccg caaaaatttt tcgcctgact gcccgtcgtc
     1561 atagctggcg tgcagggcac tcataacatc agccacggta accacaatca ctgtctgacc
     1621 gtctttcagc aggcgattcc cgatagctgc cgctaagtgg ttcttcccgg taccaggttt
     1681 tccgctgaac gcaaaatttg tacacccggt catcagttca tcagcgatgg atttcgcctg
     1741 actcaacgcg tatcgctgcc cttcgttctg cacctggtaa ttcgaaaacg agcatttgcg
     1801 gtgcaatggc tggatgccag agcgattcag aattttttcc acccgcaact gacgattctg
     1861 acggttgatc tcctcacaac gtttctggcc ttcggaaagt tgccactcgc gccactccgc
     1921 taccgtcttg aatggcgcgg ttacatgtga cggggccagt ctgcggatac gttcaagaac
     1981 gtcgcctgtc gcaatatttt tcatggtcag ttaccccctg aagcctggcg ggatcgcact
     2041 atccggtaac gagacggtgt taacctgtcg gagtaacgtc tcaggtcgaa cacctttcgg
     2101 cgcgaacaag ccctggtatt cgttggcgat gctgtgtcga atcacctgct caggtgaaaa
     2161 accctgctgg cggaattttt ccagctcccg tatcgccccg ttagcgccct gctccgttcg
     2221 aatcggtttt cgcaatgcct gcctgaactg aacccactca tgccagagtg tttccggcaa
     2281 ccagtcaggc agctcgatag cctccggttc gaatttttta gacgctcgtt tttggcgagg
     2341 gggatttagg gggagataag tatttatatc ttcctctttc tcttcctctg gtaacgcttt
     2401 ttgatccgtt tttgtaacgc tggcagcgtt accttttcgt ttcagttctc gtatttttgt
     2461 tactctctcg tttgtaaccg cccgtttttt agagcttttc ccgttatggc gctcaaagtt
     2521 aggaagcgac aacacaccat tagtttcgac cagccatcca acctgaatta acgcatcagc
     2581 aaaaccagcc ataaaagtga tgcgatctat tgcacttttt gtaacgccgc gagcgttaca
     2641 ctctgcgtta ccgtctatca tttgttgatc cgcccatgcc cagaagcgaa tgactttccc
     2701 taatgcggca tctggatcaa tattcagaat ctcagcaagc ctgaatattt ccggcttatc
     2761 cggcgtaata acttcgagct ttatccagtt tgaagccatt tgttttcacc ttgtaacgct
     2821 cgcagcgtta catttaactg ataccgaaca aaacagttcg gtacgattaa tttcaatcaa
     2881 tgcactacga cagaatcgct aggagaaccg ccgccgctga aatgtgcttt acggtaaacg
     2941 gcctggactg catcatcatg cgcatcaatt gccgtactca gtgcatcctg tgccgccagt
     3001 aatgcacggc gttccagggt atcgaagatg cagagtcggt gacgcagctc gcgaggaaga
     3061 attgccagaa ccgcagggat cagtttctga attttttccc tttgcgcatt cgtttcacct
     3121 ttcaaccaac gatgatagat attctgctga ttgttccagt ccttgcctgg taccaggggc
     3181 aattcgccgc ccccctggcg cagatattct tcagtaattg cgttagcgac ccacgcctgc
     3241 cctttttcgg ctgccagggc taacaacact gattcgatgt gctcatgctt gattttcatg
     3301 aatcaactcc catcagcttt ttcgtagtag ttttatttct gccaatagtt aaaattgcat
     3361 cggcagaaaa taatccgttt gatgcatgag cgattttttc agcgtaattt gtttcgccgg
     3421 tatattctgt gcgaggcaat tttccgttat ccatccattt gtagattgct ctttggctga
     3481 caccacaaac gtcggccaca acagaaacgc gaacagtttt gattacatct tcaagtgttt
     3541 tctggttcat atcaccctca caatgtgaac tttgagtaca tgctataaca gaactgacag
     3601 tacattcaag agcgaatatc attgaactta tggttcatga agataaagcg cgtaaagagt
     3661 tcgccagtag gcttgcgcta gcctgtgaaa acgctggtta tgaacaacat ggaaggcagg
     3721 cagaaattgc ccgtcgaatg aaattaacac caaaagcggt tagcaaatgg tttaatggtg
     3781 aaacaattcc tcgccgagag aaattaaggg aattagcaac actcattgga acaacaccaa
     3841 cctatctttt gggagaggat acagaagaaa gtggacagat acgtttctat caggagttaa
     3901 atccaagaca aaaaatcatc attgaccttc tggacgagct ccctgacagt gagacagatg
     3961 aacttttaaa aactcttgag gagaaaaaac agaagtacaa tgcaatttac gaagagttag
     4021 cacgaaagaa aaaacaaaaa gcctcttaaa ccagcataaa tccggtagcg tccccctccg
     4081 ggtttgtgct tcactttatc ccgtctcatt tttttataca taaaatgtac ttaaagtact
     4141 ttacaatgat gaacacaaag tacattatat acctaccaac ccaccccgcc ccacagaacg
     4201 ccgggcaata cttcgagtta ccaggcagtg gtcaggggtt aagtagccag cccgaggcgt
     4261 atgaacatga cggcgggatt caaattttgc agtgcagcag ttagttccgc cacccggcgt
     4321 taaggggata gataagatgg tgcattacga agtagttcag tatttgatgg attgttgcgg
     4381 tatcacttac aaccaggctg tgcaggcttt acgcagcaac gactgggatc tctggcaggc
     4441 agaagtcgct atacgtagca acaagatgtg agattcgcaa aatgcaaaaa atcgacctcg
     4501 gcaacaacga atccctggtg tgcggcgtgt tccccaacca ggatggaacg ttcactgcca
     4561 tgacgtatac caaaagcaaa acatttaaaa ccgaaactgg tgcgcgccga tggttggaga
     4621 agcacacagt aagctaacga ttaaaacgtc tactcctgct gttccagaat aacttcataa
     4681 aatgggagta tttttcggtg acgagataat aagaacagtt tgcgctatca ctctgatgtt
     4741 gaatgatgcc cttccgttct aattttttca taaccgggtt acggcaagga gaagtgataa
     4801 taagatttcc tgttttaagg aaatctttaa atacagcgat ttctttctca gataaacgaa
     4861 gcaatactcg ttgctctggt agtaatgaat aatgcttttg aatatgcgct cgcaatcttg
     4921 agaaggaaat ggcgaccacg aaagaaaagg caaaaacgat aatctgaaag agccaaggta
     4981 tttcagtata agcattgaat gcgacagtaa actctttcgg tatcagccag agagtgagac
     5041 caaaaatgat aatcgtatac ataagtcttt cgagtggctc gttagcaaaa aatttcaaca
     5101 atggagtaaa tacatccaac atatcaataa ctctcaactg taagggtatt gaaatgttaa
     5161 cacaagctct cgctgtaggg gtatagccga gaccaccgaa gcccggaggt ggtgaaataa
     5221 aaccgggcac aacacgaagg cgcatttccg atatccataa agagtcggtc ttgtctgtta
     5281 aatttaaatg gtgggagtgc gcctccggtt gtaaataacg acattgctgt gtgtagtcct
     5341 ggcggcatca gtttttttct tgaagttcgg ctgatgtccg ccctttttaa agtgaatttt
     5401 gtgatgcggt gaatgcggct aagcgcacgt ggcacagtta aaagtcatgt tagtccttat
     5461 tggtttgggt gggaaagccg actgtaattg ttaactggtt gcagtcacct ggaggcacca
     5521 ggcaccgcat caacaaagtt catttgtaaa aatggagata attatgattg cacatcactt
     5581 cggaactgat gaaataccac gtcagtgtgt gactcctggc gattatgttc ttcatgaagg
     5641 ccggacatat attgcctcgg caaacaatat taaaaagcga aaactatata ttcgtaacct
     5701 gaccacaaaa acatgcatta ctgaccgcat gattaaagtc ttcctcggtc gtgatggttt
     5761 acctgtaaag gcggagtcat ggtgatgact aagaaaataa aatgtgctta ccacctttgc
     5821 aaaaaagacg ttgaagaaag caaagctatt gaaagaatgc ttcacttcat gcacgggatt
     5881 ttatcaaaag acgaaccgag aaaatattgc agtgaagctt gtgccgaaaa agaccagatg
     5941 gcacatgaac tttaattaat tgactattcg aaactgaatt tatgccagaa atggcaggta
     6001 ttcgctcaac cttaattaag gagaaaaaca tgattaccaa ttatgaagcc actgttgtaa
     6061 ctaccgatga cattgttcac gaggtgaatc tggaaggaaa gcgcattggc tacgtaatta
     6121 aaacagaaaa taaagaaacc ccattcactg tggttgatat cgatggtcca tcaggcaacg
     6181 taaaaacact tgatgaaggt gtcaaaaaaa tgtgcctggt gcatatcgga aagaatctgc
     6241 ccacagaaaa aaaagccgaa tttctggcaa ctctaattgc aatgaaatta aaaggtgaaa
     6301 tctgaaagaa atagcctgcg tatgacgcag gctatgaaca gtgtgtatcc ggcaagatca
     6361 ttcactgaac aaaacgaatt ttaatctgag ttgaggttaa aaaacaatga gcacaaaacc
     6421 actcttcctg ttacggaaag cgaaaaaatc atccggtgaa cctgacgtcg tcctgtgggc
     6481 aagcaacgat tttgaatcga cctgtgccac tctggactac ctgatcgtta agtcaggtaa
     6541 aaaactgagc agctatttta aagctgttgc cacgaatttt cctgtcgtta atgacctgcc
     6601 cgctgaaggt gagatcgatt ttacctggag tgaacgctat caactcagca aagactccat
     6661 gacatgggaa ctaaaaccgg gagcagcacc agacaacgct cactatcaag gcaataccaa
     6721 cgtcaacggc gaagacatga ctgagattga ggagaatatg ctactcccaa tttctggcca
     6781 ggaactgccc attcgttggc ttgctcaaca cggcagcgaa aaaccggtaa cgcacgtttc
     6841 acgcgacgga ctccaggcat tacacattgc tcgggctgaa gaactaccgg ctgttactgc
     6901 cctggctgtt tcccacaaaa ccagcctgct cgacccgctg gaaattcgcg aactccacaa
     6961 actggttcgt gacactgaca aagttttccc taatcctggt aattcaaacc tgggactgat
     7021 aactgctttt ttcgaagcat acctgaacgc tgactacacc gatcgaggac tgctgacaaa
     7081 agagtggatg aagggtaatc gtgtttcaca catcactcgc acggcttccg gtgctaatgc
     7141 tggcggcgga aacctcaccg atcgcggcga aggtttcgta cacgatctga cgtcactggc
     7201 gcgcgacgta gccactggcg tactggcccg ttcaatggat ctggacatct ataaccttca
     7261 tccggcacac gctaaacgca ttgaggaaat tatcgctgaa aataaaccgc ccttttctgt
     7321 tttccgcgac aaattcatca ccatgcctgg cgggctggat tattcccgcg ccatcgtggt
     7381 tgcgtccgta aaagaagcac caattgggat cgaggtcatc cccgcgcacg tcactgaata
     7441 tctgaacaaa gtactgactg aaaccgatca tgccaaccct gatccggaaa tcgtggatat
     7501 tgcctgcggt cgctcctctg ccccgatgcc gcagcgagta acagaagaag gaaaacagga
     7561 tgatgaagaa aaaccgcaac catctggaac aacggcagtt gaacagggag aggctgaaac
     7621 aatggaacag gacgcaactg aacatcatca ggacacgcag ccgctggatg ctcagtcaca
     7681 ggtaaattct gttgatgcga aatatcagga actgcgggca gaactccatg aagcccggaa
     7741 aaacattcca tcaaaaaatc ctgtcgatgc cgataaattg cttgctgcat cacgtggtga
     7801 atttgttgac ggaattagcg acccgaacga tccgaaatgg gtaaagggga tccagactcg
     7861 cgattgtgtg taccagaacc agccagaaac ggaaaaaacc agcccggata tgaatcaacc
     7921 tgagccagta gtgcaacagg aaccggaaat agcctgcaat gcctgcggcc agactggcgg
     7981 ggataactgc cctgactgtg gtgcggtgat gggcgacgca acataccagg aaacattcga
     8041 tgaagagagt caggttgaag ctaaggaaaa tgatccggag gaaatggaag gcgctgaaca
     8101 tccgcacaat gagaatgctg gcagcgatcc gcatcgcgat tgcagtgatg aaactggcga
     8161 agtcgcagat cccgtaatcg tagaagacat agagccaggt atttattacg gaatttcgaa
     8221 tgagaattac cacgcgggtc ccggtatcag taagtctcag ctcgatgaca ttgctgatac
     8281 tccggcacta tatttgtggc gtaaaaatgc ccccgtggac accacaaaga caaaaacgct
     8341 cgatttagga actgctttcc actgccgggt acttgaacag gaagaattca gtaaccgctt
     8401 tatcgtagca cctgaattta accgccgtac aaacgccgga aaagaagaag agaaagcgtt
     8461 tctgatggaa tgcgcaagca caggaaaaac ggttatcact gcggaagaag gccggaaaat
     8521 tgaactcatg tatcaaagcg ttatggcttt gccgctgggg caatggcttg ttgaaagcgc
     8581 cggacacgct gaatcatcaa tttactggga agatcctgaa acaggaattt tgtgtcggtg
     8641 ccgtccggac aaaattatcc ctgaatttca ctggatcatg gacgtgaaaa ctacggcgga
     8701 tattcaacga ttcaaaaccg cttattacga ctaccgctat cacgttcagg atgcattcta
     8761 cagtgacggt tatgaagcac agtttggagt gcagccaact ttcgtttttc tggttgccag
     8821 cacaactatt gaatgcggac gttatccggt tgaaattttc atgatgggcg aagaagcaaa
     8881 actggcaggt caacaggaat atcaccgcaa tctgcgaacc ctgtctgact gcctgaatac
     8941 cgatgaatgg ccagctatta agacattatc actgccccgc tgggctaagg aatatgcaaa
     9001 tgactaagca accaccaatc gcaaaagccg atctgcaaaa aactcaggga aaccgtgcac
     9061 cagcagcagt taaaaatagc gacgtgatta gttttattaa ccagccatca atgaaagagc
     9121 aactggcagc agctcttcca cgccatatga cggctgaacg tatgatccgt atcgccacca
     9181 cagaaattcg taaagttccg gcgttaggaa actgtgacac tatgagtttt gtcagtgcga
     9241 tcgtacagtg ttcacagctc ggacttgagc caggtagcgc cctcggtcat gcatatttac
     9301 tgccttttgg taataaaaac gaaaagagcg gtaaaaagaa cgttcagcta atcattggct
     9361 atcgcggcat gattgatctg gctcgccgtt ctggtcaaat cgccagcctg tcagcccgtg
     9421 ttgtccgtga aggtgacgag tttagcttcg aatttggcct tgatgaaaag ttaatacacc
     9481 gcccgggaga aaacgaagat gccccggtta cccacgtcta tgctgtcgca agactgaaag
     9541 acggaggtac tcagtttgaa gttatgacgc gcaaacagat tgagctggtg cgcagcctga
     9601 gtaaagctgg taataacggg ccgtgggtaa ctcactggga agaaatggca aagaaaacgg
     9661 ctattcgtcg cctgttcaaa tatctgcccg tatcaattga gatccagcgt gcagtatcaa
     9721 tggatgaaaa ggaaccactg acaatcgatc ctgcagattc ctctgtatta accggggaat
     9781 acagtgtaat cgataattca gaggaataat tcagcctggc ggtgtaatgc accgccaact
     9841 tgaaatattt t
//
LOCUS       DAPGWD010000156         9790 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPGWD010000156 DAPGWD010000000
VERSION     DAPGWD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.157"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1181
                     /locus_tag="RHX88_003684"
     CDS             <1..1181
                     /locus_tag="RHX88_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2232331.1"
                     /translation="DNTGAEPADEAGSEQPEETAETVSPVQRLAHLTAELDALHNDRL
                     LVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLAIASLHKH
                     LLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEKH
                     TVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEM
                     ADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALLARMEV
                     VPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRAENGARML
                     ESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESVTKDETVL
                     "
     gene            1178..1921
                     /gene="tagO"
                     /locus_tag="RHX88_003685"
     CDS             1178..1921
                     /gene="tagO"
                     /locus_tag="RHX88_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HDV2232332.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            1926..3338
                     /gene="tssA"
                     /locus_tag="RHX88_003686"
     CDS             1926..3338
                     /gene="tssA"
                     /locus_tag="RHX88_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HDV2232333.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            3447..6881
                     /gene="tssM"
                     /locus_tag="RHX88_003687"
     CDS             3447..6881
                     /gene="tssM"
                     /locus_tag="RHX88_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HDV2232334.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QALRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKELNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            6892..8244
                     /locus_tag="RHX88_003688"
     CDS             6892..8244
                     /locus_tag="RHX88_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HDV2232335.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLGPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVINQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            8268..8750
                     /locus_tag="RHX88_003689"
     CDS             8268..8750
                     /locus_tag="RHX88_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDV2232336.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            8794..9708
                     /locus_tag="RHX88_003690"
     CDS             8794..9708
                     /locus_tag="RHX88_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232337.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT         2375 a         2624 c         2710 g         2081 t
ORIGIN      
        1 aggataatac cggtgccgaa ccggctgatg aagctggcag cgaacagccg gaagagaccg
       61 ctgaaacagt ttccccggta caacgtctgg cacatctcac tgccgaactg gacgccctgc
      121 ataacgaccg gttgctggtt tccccgcacg tcgataaaaa acagattgcg gcggtgattg
      181 ccgaatggac cggcgtgccg cttaaccgcc tgtcgcagaa tgaaatgtcg gtcatcaccg
      241 acctgccaaa atggctcggc gacaccatca aaggccagga cctggcgatt gccagcctgc
      301 acaaacacct gctgaccgca cgcgccgacc tgcgccgtcc gggacgcccg ctgggcgcgt
      361 tcctgctggc tggccccagc ggcgtgggta aaactgaaac cgtcctgcaa ctggcagagc
      421 tgctctacgg cggtcgccag tacctgacca ccatcaatat gtccgagttc caggagaaac
      481 ataccgtctc gcgactgatt ggctcccctc cgggctacgt tggctacggt gaaggcggcg
      541 tactgaccga agcgattcgc cagaaaccct actcggtagt actgctcgat gaagtggaaa
      601 aagcgcaccc ggatgtgctc aacctgttct accaggcgtt cgataagggc gaaatggcag
      661 acggtgaagg ccgcctgatt gactgcaaaa atatcgtctt cttcctgacg tccaacctcg
      721 gctaccaggt aatagtcgag catgccgatg acccggaaac catgcaggaa gtactgtatc
      781 cggtgctggc cgacttcttc aaacctgccc tgctggcgcg tatggaagtg gtgccgtacc
      841 tgccgctgtc gaaagagacg ctcgccacca tcattgccgg gaaactggcc cgcctggata
      901 acgtgctgcg cagccgcttt ggcgcggagg tgattataga accggaagtg acggacgaaa
      961 tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat gctggaatcg gtcatcgacg
     1021 gcaatatgct gccgccgctc tccctgctgc tgttgcagaa aatggcggcg aatacggcgg
     1081 ttgcccggat tcggttgtcg gcagtggacg gcgcatttac ggcagacgtg gaagatgctc
     1141 agaacgacga gtccgtcaca aaggatgaaa cggttttatg agcaggttgc gcgttttccg
     1201 gcagacagga tggtttatcg cgggcctgat gacgggctta cccgccactg ccgcgcccgc
     1261 agaagcatcg tcaatggccg gtgtggcggt cgccgtcgcc accacgaccc cgccggatgc
     1321 gacagccacg ttacaggcca tgcagtcctg tcggcgggag tccgcggcgc tggagcggct
     1381 tgactgctat gaccgcctgc tggcaccgct gtccccgtca gggtttgacg gggcgctggt
     1441 caaagccggt tttgtgggcg aagcctggac ccgcgccacg gaacaggaaa aacaccgcca
     1501 gggcaacacc acagagctgc tggtgacgca ggtgccgggg gagcgcccga cggtggtgat
     1561 aaccaccccg gctatcggtc atgttccgcc acgcccggtg ctgatgttca gttgcgtgga
     1621 caatatcacc cggatgcagg tggcgctgat gcaccctctg gatgtccatg atatcgccgt
     1681 cactctgaat gccgacaacc gggcattgcg cagccactgg tttgtgcgcg agaacggcac
     1741 cctgctggag tccagccggg ggttatccgg gattgatgaa attaagcaac tgttcggcgc
     1801 aaaaacgctg acggtggata ccggcgcaga caatgccgcc ggaaagctga cctttaacat
     1861 tgacgggctg gcaagggcga ttgctcccct gcgcgatgcc tgccactggg cgggagaata
     1921 aaaatatggc aatggactta cgcgatccga atgtctggat atcgcacctg ctggaaaacc
     1981 tgccggaaga gaaactggca tcggcgctga aagatgacaa cccgaactgg gagtatatcg
     2041 acggcgaaat cgtcaagctg ggatcgctgg cccacagtca actggatatc ccggaactcc
     2101 agcgccgggg gctggtcatt ctggcttcgg agagtaagga ttttcgtctg ctggcgcatc
     2161 tgctgagaac cctgcaacac gccggtgatc ctcatctcgc attgcgcctg ctggcgctgt
     2221 atgtggagca ttactgggcg gtggctgcgc cgcagaatat ggcgcataaa aaacgctttg
     2281 ccagccaggt cattaaacgc tttgaaacag gtattgaagt tttttcacag aacgccgcca
     2341 cagcgcagcg cgatgcgctg tcaggggaac tggcaaaact ggcgcaatgc tggcagtcac
     2401 ataacgcccc ggaactggca caggctactg atgatctttt tgccctgtac cagcgtgcgt
     2461 ttaatcgtgc ggcaccggct cctgtcccca ctccggcggc ctccggtagt tcaccacaga
     2521 ccaccgccac gtctgaaagc ggcgtgacgc aacccagtgc tccggctccc caaatcgtca
     2581 tcgacagtca cgacgacaaa gcctggcgcg acacgctgtt aaaagtggcg gccattttat
     2641 gtgaacgcca gccggattca ccgcagggct accgtctgcg ccgccatgcg ctttggcaga
     2701 acatcaccag tacgccccag gcggaaagcg atggccgtac tccactggct gcggtctccg
     2761 ccgatatggt ggcagattac catgctcagc tcggtagtgc cgatatggcg ctgtggcagc
     2821 aggtggagaa aagcgtactg ctggctcctt actggctgga tggtcactgc ctttctgcac
     2881 agacggcgct gcgtctgggt tacaaacagg tggcagacgc catccgcgat gaggtcatcc
     2941 gcttccttga gcgtctgccc cagcttaccg ggctgctgtt taatgaccac acaccgttta
     3001 tcagtgagca gacgaaacaa tggctggcag cttcgcccga cgccaaagtt gcaccggttg
     3061 cgcaaatcgg tgaggaatcg aaggcagcca gagcctgttt tgctgagcag ggtctggagg
     3121 cggcgctgcg atatctggac atgctacccg aaggcgatcc ccgcgatcag tttcaccgcc
     3181 agtaccttgc cgcacagttg acggaagagg cggggctggt acagcttgcg cagcagcagt
     3241 accggatgtt gttcaggatg ggattgcaga tgatggtggc agactgggag ccgtccctgc
     3301 ttgagcagct agaacaaaaa ttcacggcag aacaataaaa gagaacagga gtttttgtgt
     3361 tcagattacc aacaccccga ctactcagcg gactcaaatc agccctgcga ccggcgatgc
     3421 cccggtttaa agtctctgct ttctggctgc tgatactggc gtggattttt ctgctggtgt
     3481 ggatctggtg gaaaggcccg acgtggacgc tgtatgaaga acagtggctc aaaccactgg
     3541 cgaaccgctg gctggcaacg gcggcgtggg ggattattgc cctgatgtgg cttaccgtcc
     3601 gggtgatgaa gcgcctgcaa cagcttgaaa aaatgcaaaa gcaacagcgc gaggaagccg
     3661 ttgatccgct cagcgtggaa ctgaacgccc agcagcgtta tcttgaccgc tggctgctgc
     3721 gcctgcaacg ccatctcgac aaccgccgtt tcctgtggca gttgccgtgg tatatggtca
     3781 tcggcccggc gggcagtggc aaaacgacac tgctgcgcga ggggtttccg tccgacatta
     3841 tttatgcccc ggagggcgca cgcggcgcag aacaacgcct gtacctcacg ccccatgtcg
     3901 gtaaacaggc ggtgatcttt gatatcgacg gcacactctg cgctcccgct gatgcggata
     3961 tcctgcatcg ccgtctgtgg gaacatgccc tcggctggct gaaagaaaag cgcgcgcgcc
     4021 agccgctgaa tgggattatt ctgacactcg atttacccga tctgcttacc gcagacaaac
     4081 gccgccgcga gcatctgtta caggcgctgc gcagccgctt gcaggatata cgccagcatc
     4141 ttcactgcca gttaccggtt tacgtggtac ttacccggct tgatttattg cagggttttg
     4201 ccgccctgtt tcagtccctg aacagacagg atcgcgatgc gattctgggc gtcacgttca
     4261 cccgccgtgc ccatgaaaat gatgactggc gaacagagtt gaatgctttc tggcagacat
     4321 gggtggatcg aatgaatctg gcgttgccgg atctgatggt cgctcagact cacacccgcg
     4381 cgtctttatt cagtttttcc cgccagatgc agggaagccg tgaaccgctg gtgtcactgc
     4441 ttgagggtct gcttgatggc gaaaatatga acgtgatgct gcgtggtgtc tatctcacct
     4501 cttcgcttca gcgtggacag atggatgata tattcaccca gtctgccgcc cgccagtacc
     4561 ggctgggcaa taacccactg gcgtcctggc ccctggtgga caccgcgcct tatttcaccc
     4621 gcagcctgtt cccgcaggca ttactcgcag agcctaatct ggcaacagag agccgcgcct
     4681 ggctgatacg ttcccgtcgc cgcctgacgg ttttctctgc cacaggcggc gtggcagcac
     4741 tgctgctcat caccggctgg catcactatt acaacggtaa ctatcagtct ggcatcaccg
     4801 tgcttaagca ggccaaagcc tttatggacg tgccgcctcc gcagggggaa gatgactttg
     4861 gcaacctgca actgccgctc ctgaacccgg tacgcgatgc cacactggcc tatggcgact
     4921 ggggcgaccg cagccgtctg gccgatatgg gactgtacca gggacgacgt atcgggcctt
     4981 atgtggaaca gacctatctg caactgctgg agcaacgtta cctgccctcg ctgtttaacg
     5041 ggctggtcaa agagctgaac gccgcgccgc cggagagtga agaaaaactc gcggtgctgc
     5101 gcgtgatgcg aatgctggag gacaaaagcg gacgtaacaa tcaggtggtg aagcagtata
     5161 tggcaaaacg ctggagcgaa aagttccacg gtcagcgcga tatccaggca caactgatgt
     5221 cccatcttga ctacgcgctg gctcatactg actggcacgc agagcgtcag gcgggcgacg
     5281 gtgacgccat cagccgctgg acgccatatg acaagcccgt ggtatcagca cagaaagaac
     5341 tgagcaaact gcctgtctac cagcgggttt accagagcct gaaaacgcgg gcgctgggcg
     5401 ttcttcctgc cgacctcaat ctgcgtgacc aggtagggcc aacctttgac caggtgttta
     5461 catctgccga tgacaacaaa ctggttgttc cacagtttct tacccgttac ggcctgcaaa
     5521 gctattttgt aaaacagcgc gatgaactgg ttgaactgac ggcgatggat tcctgggtac
     5581 ttaaccttac ccgcagcgtg aaatacagtg acgccgaccg cgcggaaatc cagcgccagt
     5641 tgaccgagca gtatatcagc gactacaccg ccacctggcg ggccgggatg gacaatctga
     5701 atatccgcaa ttttgagtcc atcggacaac tgaccggggc gctggagcag gttatcagcg
     5761 gcgaccagcc tttgcagcgg gcgctaaccg tgctgcgtga caacacacag ccaggcgtct
     5821 tttctgaaaa actctctgcc aaagaacggg aggaagccct ggcagagccg gattaccagt
     5881 tactcacccg cctcgggcat gaattcgccc cggaaaacag taccctggca gtacagaaag
     5941 acaaagaaag cacgatgcag gccgtgtatc agcaactcac cgagttgcac cgctacctgc
     6001 tggcaatcca gaacgcgcct gtaccaggaa aatcggcgct gaaagccgtg cagttacggc
     6061 ttgatcagaa cagcagcgat ccgatattcg ccacccgcca gatggcaaaa acgctgcctg
     6121 ctccgctcaa ccgctgggtt ggcaggctgg ctgaccaggc ctggcatgtg gtgatggtgg
     6181 aggctgttca ttatatggaa gtggactggc gcgacagcgt ggtgaaaccg tttaacgagc
     6241 aactggcaaa taactatccg tttaatccgc gttctgcaca ggatgcctca ctggatgcct
     6301 tcgaacgctt ctttaaaccg gatggcatac tggatacctt ctaccagcag aacctgaagc
     6361 tgtttatcga taatgacctg agtctggagg atggcgataa caacgtcatt attcgcgaag
     6421 atattattgc gcaactggaa actgcgcaga aaatccgtga catcttcttc agcaaacaga
     6481 acggtctggg aacatccttt gccgtggaaa cggtatcgct ttcaggcaat aaacgccgca
     6541 gtgtactgaa ccttgacggt cagttagtcg attacagcca gggccgtaac tataccgccc
     6601 atctggtctg gcctaacaac atgcgcgaag gcaacgaaag taagctgacg ctcatcggca
     6661 ccagcggcaa cgcgccgcgc agtatcagct tcagcgggcc gtgggcgcag ttccgcctgt
     6721 tcggggccgg acaactgacc ggagtacagg atggcaactt taccgtgcgc tttagcgtgg
     6781 acggtggcgc gatgacctac cgtgtgcata ccgacacgga agataacccg ttcagcggtg
     6841 ggttgttcag ccagtttggt ctgtcagaca cactgtactg aggaggcagg gatgaatagt
     6901 aacgtactga cacaaactat cgttaccggc agtgacccgc gcgggctgcc ggaattcagc
     6961 gccatccgcg aggaaataaa caaagccagc cacccgtcac agcctgagct gaactggaaa
     7021 ctggtggagt cgctggcgct ggcgattttt aaagccaacg gtgtggattt acacaccgcc
     7081 acctactata cgcttgcccg gacacggaca cagggactgg cgggattctg cgaaggtgcg
     7141 gaactgctgg cggcaatggt aagccacgac tgggataagt tctggccgca gggcggcccg
     7201 gcgcgtactg aaatgctgga ctggtttaac tcccgaaccg gcaatattct gcgtcagcaa
     7261 atctcctttg cggaatccga cctaccactg atctaccgca cagagcgggc attgcagctt
     7321 atctgcgata agctccagca ggtggaactg aagcgcgttc cgcgcgtgga gaatctgctc
     7381 tattttatgc agaacacgcg taaacggctt ggaccccagc tgaagagtaa cactgagaac
     7441 gccgcacaga ccacggtcag aacgctgatt tatgccccgg aaacacaggc atcttccaca
     7501 ccagaagcgg tagtgcctcc cctgcccggc ctgcctgaga tgaaagtgga agttcgcagt
     7561 ctgacagaga atcccccaca ggccagtgtg ataaatcaag gcagtacggt aagagggttt
     7621 atcgcaggga tcgcctgttc agtggctgtc gcctcagcat tgtggtggtg gcaggtctat
     7681 ccggtgcagc agcaactgtt acaggttaac gacaccgctc agggcgcagc aacggtgtgg
     7741 atggcctcac ctgaactcga aaactatgag cgcaggctgc aacaacttct tgatacctcc
     7801 ccggtacagc cgctggaaac cgggatgcag atgatgcgtg ttgccgacag tcgctggccg
     7861 gaaagcctgc aacagcaaca ggcctcgaca caatggaatg aggcactcaa aacccgcgca
     7921 cagagtagcc cgcagttgcg tggctggttg cagacccgcc aggacttaca tgcttttgca
     7981 gatctagtga tgcagcgcga gaaagaggga ctaacccttt cctatatcaa aaatgtcatc
     8041 tggcaggcgg agcggggact ggggcaggaa acacccgttg agtctctgtt gacgcagtac
     8101 catgatgccc gtgcgcagaa gcagaataca gatgcgctgg aaaaacaaat taatgagcga
     8161 ctcgaaggcg tgttaagccg ctggctgctg ctgaagaata acgtcatgcc agaggcggca
     8221 accggtacca cggccgaaaa ataattattt aattaaagga gattatcatg gcaaatataa
     8281 gttatttatc tttgagtgga gagacacaag ggctaatttc agcaggttgc tcaacgcttg
     8341 attctgtcgg aaacaaagcg cagccagaac ataaagatca gatcatggtt tatgcgttaa
     8401 tgcattccat ctcaagaagt caaaatgtca atcatcatga attaattatc accaaaccgg
     8461 ttgataaatc ctcaccattg ctggcaaaag cacttagtga caatgaaaaa atggcaatat
     8521 gtgaatttat actctaccga accagtaaag cgggtattta ccagccttat tataaaataa
     8581 atctgtcaaa agcccgaata tcaagtattg attttgtcac accgcatgct gtcctggaaa
     8641 aagaactgga accacaggag agaattgcct ttatttatga agatatttcc tgggagcata
     8701 cgttagccgg tacgaatgca atgagtaaat ggcaggatcg tgtacagtaa cttactgtaa
     8761 taacataata aattactatt gcggagagat aaaatggctg gtttaaggaa taacaacaac
     8821 acacagaatg cacagtgggc tgattatgtg ggtgacattc ttcggggggc tcagccgata
     8881 aaccagctag tacctcagca cccttatctt aacgacgtcc cactcattga tgagttaagg
     8941 catcagaaca ctcatcatgt tattctcttg acacttgatg ttgcaaaaaa aatactcagc
     9001 ccaataacat catttgatta cattcatttt atcactacac atccttcagg tataaaagat
     9061 acactcgcct ggttagttaa cgcgagtaaa ttaatgactg agtttgatga caatgggaaa
     9121 attatattta accttaatgc cttaaagtat acaaaagcaa gttactttga aatacttgga
     9181 gagaaatata taaaaatcac aacatccagc ccctggcttt tagaaaagct cggtaaatat
     9241 attttctctt cacgagcgcc tcaagttctg gaactcgcta ttggctggcg aggagcttta
     9301 tctgaatcaa taaaaggagt taagttctgt atctggtttt cagtagcctg gagaaccata
     9361 gaatttatta tgagttcaga gcgagatctg gttaattttc ttggcgattt ttcaatggat
     9421 gtggccaaag cagttatagc cggtggtgtt gcaaccgcta ttggaagcct ggcttctttt
     9481 gcctgtgtta gctttggctt tccagtaatt cttgtcggag gagcaatttt actgacaggg
     9541 atagtgtgta cagttgtttt aaatgaaatc gatgctcaat gccatttatc agaaaaatta
     9601 aaatatgcaa ttagagatgg actaaaacgg caacaggaac ttgataaatg gaaaagggaa
     9661 aacatgactc catttatgta tgttcttaac actccacccg tgatatgagg attaaccatg
     9721 cgtttacatg ttaaattaaa agagtttctt tcgatgtttt ttatggccat attatttttc
     9781 cctgcattca
//
LOCUS       DAPGWD010000157         9744 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPGWD010000157 DAPGWD010000000
VERSION     DAPGWD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.158"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..915
                     /locus_tag="RHX88_003691"
     CDS             <1..915
                     /locus_tag="RHX88_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HDV2232338.1"
                     /translation="ARVVSCISPAKFHECFINWMRDCHSSNDKDVIAIDGKTLRHSYD
                     KSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGC
                     QKDIAEKIQKQGGDYLFAIKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREE
                     IRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTA
                     EKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVF
                     KAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1184..3421
                     /gene="nrfB"
                     /locus_tag="RHX88_003692"
     CDS             1184..3421
                     /gene="nrfB"
                     /locus_tag="RHX88_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDV2232339.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3408..6380
                     /gene="nfrA"
                     /locus_tag="RHX88_003693"
     CDS             3408..6380
                     /gene="nfrA"
                     /locus_tag="RHX88_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDV2232340.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6381..7271
                     /locus_tag="RHX88_003694"
     CDS             6381..7271
                     /locus_tag="RHX88_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDV2232341.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7454..8215
                     /gene="envY"
                     /locus_tag="RHX88_003695"
     CDS             7454..8215
                     /gene="envY"
                     /locus_tag="RHX88_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDV2232342.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8728..9681
                     /gene="ompT"
                     /locus_tag="RHX88_003696"
     CDS             8728..9681
                     /gene="ompT"
                     /locus_tag="RHX88_003696"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HDV2232343.1"
                     /translation="MRAKLLGIFLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT         2593 a         2426 c         2426 g         2299 t
ORIGIN      
        1 gccagagttg tatcctgtat cagtcctgca aaatttcatg agtgctttat taactggatg
       61 cgtgactgcc attcatcaaa tgataaagac gtcattgcaa ttgatggaaa aacgctccgg
      121 cactcttatg acaagagtcg ccgcagggga gcgattcatg tcattagtgc gttctcaacg
      181 atgcacagtc tggtcatcgg acagatcaag acggatgaga aatctaatga gattacagct
      241 atcccagaac ttcttaacat gctggatatt aaaggaaaaa tcatcacaac tgatgcgatg
      301 ggttgccaga aagatattgc agagaagata caaaaacagg gaggtgatta tttattcgcg
      361 ataaaaggaa accaggggcg gctaaataaa gcctttgagg aaaaatttcc gctgaaagaa
      421 ttaaataatc cagagcatga cagttacgca atcagtgaaa agagtcacgg cagagaagaa
      481 atccgtcttc atattgtttg cgatgtccct gatgaactta ttgattttac gtttgaatgg
      541 aaaggtctga agaaattatg cgtggcagtc tcctttcggt caataatagc agaacaaaag
      601 aaagagcccg aaatgacggt cagatattat atcagttctg ctgatttaac cgcagagaag
      661 ttcgccacag caatccgaaa ccactggcac gtggagaata agctgcactg gcgtctggac
      721 gtggtaatga atgaagacga ctgcaaaata agaagaggaa atgcagcaga attattttca
      781 gggatacggc acatcgctat taatattttg acgaatgata aggtattcaa ggcagggtta
      841 agacgtaaga tgcgaaaagc agccatggac agaaactatc tggcgtcagt ccttgcgggg
      901 agcgggcttt cgtaatcttg ccctgcctgc tcacctcctc caggcacttc cactacatga
      961 tgacctgatc ggtgccacca ctcctcttca ctatgacgtt aacctcccct ccttgacacc
     1021 acgcttttgt ctacattcta cgcaaacggc aacttttata accagtgtaa aaataacgtg
     1081 ccgcaatagt catacctatt aatggtaaaa agctgtcaca attcataaaa aaccttaata
     1141 tacgccaccc taaacataac cagcgttaat gtaaggtttt tgtgtggact ggcttcttga
     1201 tgtttttgca acctggctct acggcttaaa agtaatcgcg ataacgttag cggtcatcat
     1261 gttcatcagc gggctggacg atttttttat tgatgtcgtc tactgggtac gccgcattaa
     1321 acgcaagttg agtgtttatc gccgctaccc gcgaatgagt taccgcgaac tgtataaacc
     1381 agatgaaaaa ccgttagcga ttatggttcc ggcgtggaat gaaacgggcg tcatcggcaa
     1441 tatggccgag ctggcggcga ccacgctcga ctacgaaaac tatcatatct ttgttggcac
     1501 ctaccccaac gaccccgata ctcaacgtga tgttgacgaa gtgtgcgctc gctttccgaa
     1561 cgtgcataag gtagtctgcg cgcgtcctgg tcccaccagc aaagccgact gtctgaacaa
     1621 cgtgctggac gccatcaccc agtttgaacg tagcgccaat ttcgcttttg ctggttttat
     1681 tctgcatgac gccgaagatg tgatttcacc gatggaattg cgtctgttca actatctggt
     1741 cgagcgtaaa gatctgattc agatcccggt gtatccgttc gaacgcgaat ggacgcactt
     1801 caccagcatg acttacattg atgagttttc agagctgcat ggcaaagatg ttccggtgcg
     1861 tgaagccctc gccggacaag tgcccagcgc aggcgtcggc acctgtttca gccgccgcgc
     1921 cgtgaccgct ctgttagctg acggtgacgg tattgctttc gacgtgcaga gtctgactga
     1981 agattacgac attggtttcc gcctgaaaga gaaaggtatg acggaaattt ttgtccgttt
     2041 tccggtggtg gacgaagcca aagaacgcga gcagcgcaaa tttttacagc acgcacggac
     2101 gtcaaacatg atctgcgtgc gcgaatattt ccccgatacc ttttcgactg cagttcgaca
     2161 aaaatctcgc tggatcatcg gcattgtttt ccagggcttt aaaacccaca aatggacctc
     2221 cagcctgacg ctgaactact ttctctggcg cgaccgcaaa ggggcaatca gtaactttgt
     2281 cagcttcctc gcgatgctgg tgatgctcca gcttttgctg ttgctggcgt atgaaagttt
     2341 gtggcccaat gcctggcatt tcctttctat ttttagcggc agcgcatggt taatgaccct
     2401 gctgtggcta aactttggct tgatggttaa ccgcatcgtg cagcgggtga ttttcgtcac
     2461 tggctactac ggtctgacgc aggggctgct atctgtcctg cgtcttttct ggggcaacct
     2521 gattaacttc atggccaact ggcgcgcgct aaaacaggta cttcaacacg gcgatccacg
     2581 tcgcgtcgcg tgggataaaa caacgcatga cttccccagc gtgactggcg atacccgctc
     2641 gttgcgcccg ttaggccaaa ttctgctgga aaatcaggtc atcactgaag aacaactcga
     2701 tacagcactg cgtaatcgcg tcgaaggtct acgcctgggc ggttcaatgc tgatgcaggg
     2761 gctgattagc gccgagcagc tggcacaggc gctggcagag caaaacggcg tggcgtggga
     2821 atccatcgat gcctggcaga tcccgtcctc gctgattgcc gaaatgccag cctccgtagc
     2881 gctgcattat gcagtactgc cgctgcgtct ggaaaatgat gagttaattg tcggcagtga
     2941 agatggtatt gacccggttt cgctggcggc cctgacgcgt aaagtcggac gcaaagtgcg
     3001 ttacgtcatt gttctgcggg gacaaattgt cacggggtta cgtcactggt atgcacgccg
     3061 acgcggtcac gatccgcggg caatgttgta caatgcggtt cagcatcagt ggctcacgga
     3121 acagcagacc ggtgaaatct ggcggcaata tgtgccgcat cagttcctgt tcgccgaaat
     3181 actgaccacg ctcggtcata ttaatcgttc agcaattaac gtgttgttat tgcgccatga
     3241 acgcagttct ctgccgctcg gcaagttttt ggtcaccgaa ggcgttatca gccaggaaac
     3301 gttggatcgc gtcctgacaa ttcaacgcga attacaagtt tcgatgcaat cactattact
     3361 caaagcaggt ttaaacacag aacaggttgc gcaactggag tccgaaaatg aaggagaata
     3421 accttaatcg cgtcatcgga tggtctggtt tactgctgac gtctttattg agtaccagcg
     3481 cactcgcaga caatatcggc accagcgcag aagagctggg gctgagcgat tatcgccatt
     3541 ttgttattta tccccgtctc gataaggcgc tgaaggcaca gaaaaataac gacgaagcaa
     3601 ccgccatccg cgaatttgaa tatatacacc agcaggtgcc ggataatatt ccgctgactt
     3661 tataccttgc ggaagcctat cgccattttg gtcatgatga ccgggcgcgg ctgttgcttg
     3721 aggatcaact gaaacgtcac ccaggagatg cccgacttga gcgcagtctg gcggctattc
     3781 cggttgaagt gaaaagcgtt acgactgttg aagaactgct tgcccagcaa aaagcgtgcg
     3841 acgctgcgcc gaccctgcgt tgtcgcagtg aagtcgggca gaatgccctg cggctggcac
     3901 aattacctgt cgccagagcg caactgaacg atgcgacgtt tgctgcatcg ccagaaggaa
     3961 aaacgctgcg aaccgatctg ctgcaacggg caatctacct gaaacaatgg tcccaggcag
     4021 atacgctata caatgaagca cgccagcaga acacattaag cgcggcagaa cgccgtcagt
     4081 ggtttgacgt gcttcttgcc gggcagctgg acgatcggat cctggcactg caatcacagg
     4141 ggatcttcac cgatcctcag tcatatatta cttacgcgac cgcgctggct tatcgtggcg
     4201 aaaaagcacg cctccagcat tatctcaatg aaaataagcc gctgtttacc acggacgcac
     4261 aagagaaaag ttggctctat ctgttatcta aatacagcgc caaccccgtt caggcattgg
     4321 cgaattatac ggtgcagttt gccgataacc gccagtatgt tgttggcgcg acgctaccgg
     4381 tgctgttaaa agaaggtcag tacgacgcag cgcaaaaact gctcgccacc ctccccgcca
     4441 atgaaatgct tgaggagcgt tatgctgtca gcgtggcgac ccgtaacaag gctgaatctc
     4501 tgcgtctggc acgattgctg tatcagcaag aaccggcaaa tcttacccgc ctggatcaac
     4561 tgacctggca actgatgcag aacgagcagt cacgcgaagc tgccgattta ttgctgcaac
     4621 gctatccttt ccagggcgat gcgcgtgtca gccagacttt aatggcgcga ctggcgtctc
     4681 tgctggaaag tcatccttac ctggcaacgc cggcgaaggt ggcgatttta tcgaaaccct
     4741 taccgctggc ggagcaacgt cagtggcaaa gtcagttgcc gggtattgca gataattgcc
     4801 cggcaatagt tcgcttgctg ggcgatatgt cgccttccta cgatgctgcc gcctggaacc
     4861 gtctggcaaa gtgttatcgg gacacgctac ccggtgtggc gctgtatgca tggcttcagg
     4921 ccgaacaacg acaaccgaac gcctggcaac atcgtgcggt agcctatcag gcgtatcagg
     4981 ttgaggacta cgccaccgca ctggcggcct ggcagaaaat cagtcttcac gacatgagca
     5041 atgaggatct gcttgctgct gccaataccg cccaggcggc aggaaatggt gcagctcgcg
     5101 atcgctggct acaacaggca gaacaacgtg gactgggaaa caatgccctc tactggtggc
     5161 tgcatgcgca acgttacatt cctggtcagc cggaactcgc actgagcgat ctcacgcgct
     5221 caatcaatat tgcgccttct gccaacgctt acgttgcgcg ggcgacaatt tatcgccaac
     5281 gtcataatgt cccggcggcg gtgagtgatt tgcgcgccgc gctggaactg gaaccgaata
     5341 atagcaacat ccaggcagcg cttggttacg ccttgtggga tagcggtgat atcgcacagt
     5401 cgcgggaaat gctcgaacag gcgcataaag ggcttccgga cgatccggca ctgatccgac
     5461 aactggccta tgtgaaccag cgtctggatg acatgcctgc gacgcagcat tacgcccggc
     5521 tggtgattga tgacattgat aatcaggcgc tgataacccc actgacccca gagcaaaatc
     5581 agcaacgctt caatttccgc cgtttgcatg aggaggtcgg tcgccgctgg acgttcagtt
     5641 tcgattcttc tatcggcttg cgttccgggg caatgagcac cgctaacaat aatgtcggcg
     5701 gcgcagcgcc agggaaaagc tatcgtagct acgggcaact ggaagccgag taccgcatcg
     5761 gacgcaatat gctgctggaa ggcgacctgc tctcagttta tagccgcgtc tttgccgata
     5821 ccggagaaaa cggggtgatg atgccggtga aaaatccgat gtccggcacc ggtctgcgct
     5881 ggaagccgct gcgcgatcag atctttttcc tcgccgtcga acagcagttg ccgctgaacg
     5941 ggcaaaatgg cgcatccgat accatgctgc gcgccagcgc ctcattcttt aatggcggca
     6001 aatacagcga cgaatggcac ccgaacggtt caggctggtt tgcccaaaac ctgtacctcg
     6061 atgcggcgca atatatccgc caggatattc aggcgtggac ggcagattat cgcgtcagct
     6121 ggcatcagaa ggtagctaac ggacagacta ttgagcctta cgctcacgtt caggacaacg
     6181 gctatcgtga taaaggcact cagggcgcgc agcttggcgg agtcggggtc cgctggaata
     6241 tctggaccgg cgagacgcac tacgacgcct ggccgcacaa agtcagtctc ggcgtcgagt
     6301 atcaacatac ctttaaggcg attaatcaac gtaacggaga gcgcaacaac gcgtttctca
     6361 ccattggagt gcactggtaa atgcgtaagt tcattttcgt attgctgaca ctgcttttgg
     6421 tcagcccttt ttcctttgcg atgaaaggta ttatctggca accacaaaac cgagatagtc
     6481 aggttaccga tacccagtgg caggggctga tgagtcagtt acgtttgcaa ggcttcgata
     6541 ccctggtttt gcaatggacc cgttacggcg atgcattcac ccagccagaa cagcgcgcgt
     6601 tattgtttaa gcgtgccgca gctgcgcaac aggctggcct gaagctgatt gtcgggctga
     6661 acgccgatcc ggaatttttt atgcaccaga aacagtcgtc cgcagcgctg gaaagctatc
     6721 ttaatcgcct gctggctgcc gatctccagc aagccagatt atggagcgcc gcgcctggcg
     6781 taacgccgga tggctggtac atcagcgcgg aaattgacga cctgaactgg cgcagcgaag
     6841 ctgcccgtca gcctttgcta acatggttaa acaacgcgca gcggctgatt agcgatgttt
     6901 cagcaaaacc ggtttatatc agtagttttt tcgccggaaa catgtcgccc gatggctatc
     6961 gccaactgct ggaacaagtt aaagcaactg gcgttaatgt ctgggtacag gatggcagcg
     7021 gcgtggataa acttaccgct gaacagcgtg aacgttatct acaggccagc gccgattgcc
     7081 aaagtcccgc ccctgccagc ggcattgttt atgaactgtt tgtcgccggc aaaggcaaaa
     7141 cctttacagc gaaaccgaaa ccggatgcag agatcgcctc gctgttagcg aaacgttcct
     7201 cttgcggtaa agacactctc tatttctctc tgcgctattt gcccgtcgcg cacggcattc
     7261 tcgagtatta aatctcctcc aggtaagtcg ggtacgacct ggcttacctc tttcgctctc
     7321 gtcagaattc catcaagatt aattgctaaa aagcgtgcta aagaaagaag atcgttatcg
     7381 gcgaacgggc cgatcactca tgcaggaagc atagatcctt tgagcggagt ttctgatatc
     7441 tgaggagtgc gaaatgcaat tgagcagcag tgaaccttgc gtggtgatcc tgaccgaaaa
     7501 agaggtagag gtaagcgtca ataaccatgc tacgtttacc cttccgaaaa actacctggc
     7561 cgccttcgcg tgcaacaata acgtcattga actctcaacg ttaaatcacg tattaatccc
     7621 ccacatcaac cgtaacatca tcaacgatta tctgttgttt ttaaataaga acttaacctg
     7681 tgtaaagccc tggtcgcggc tggcaacccc ggttatcgct tgtcatagcc gtacaccgga
     7741 agtgttccgg ctagccgcca accacagcaa gcagcaaccc agcaaaccct gcgaagcgga
     7801 gttgacacgc gcattacttt ttaccgtatt gtctaacttt cttgagcaat cgcggtttat
     7861 agccctactg atgtatatct tacgcagcag cgtccgcgac agcgtctgcc gcattattca
     7921 aagcgatatt cagcattact ggaatctgcg aattgtcgcc agttcgctat gtttaagccc
     7981 cagcctgctc aaaaagaaat taaaaaacga aaataccagc tatagccaga ttgtcacaga
     8041 gtgtcgtatg cgttacgccg tacagatgtt attgatggat aacaaaaata tcactcaggt
     8101 ggcgcaatta tgtggctata gcagcacgtc atactttatc tctgttttta aggcgtttta
     8161 cggcctgaca ccgttgaatt atctcgccaa acagcgacaa aaagtgatgt ggtgaagggc
     8221 aaagcggaaa cggataagac gggcataaat gaagaagaaa tggcgcgccc tgcaggattc
     8281 gaacctgcgg cccacgactt agaagttcct agaacgacat ttaagtcaac aacttaccgc
     8341 gccatctctg cgctcacacg tcccactacc tcaaaacatg taaagccttg caagccattg
     8401 tgaggcctta tgtgtctcag ttttgtccct cttttttgta ctaaaaaaca tagtaattga
     8461 ggataaacct catgctattt tcgcttatat gcctctaaag gcatggcact taaatagata
     8521 aaagcaccac aaaagcataa aaaaaccaca cagtaaaacc gcaatatgaa acaataacag
     8581 ataattaaac caaaaacaga tagctcattg tgataatcat tcaatactaa acaaaatata
     8641 aacagtggag caatatgtaa ttgactcatt aagttagata taaaaaatac atattcaatc
     8701 attaaaacga ttgaatggag aacttttatg cgggcgaaac ttctgggaat attcctgaca
     8761 acccctattg cgatcagctc ttttgcttct accgagactt tatcgtttac tcctgacaac
     8821 ataaatgcgg acattagtct tggaactctg agcggaaaaa caaaagagcg tgtttatcta
     8881 gccgaagaag gaggccgaaa agtcagtcaa ctcgactgga aattcaataa cgctgcaatt
     8941 attaaaggtg caattaattg ggatttgatg ccccagatat ctatcggggc tgctggctgg
     9001 acaactctcg gcagccgagg tggcaatatg gtcgatcagg actggatgga ttccagtaac
     9061 cccggaacct ggacggatga aagtagacac cctgatacac aactcaatta tgccaacgaa
     9121 tttgatctga atatcaaagg ctggctcctc aacgaaccca attaccgcct gggactcatg
     9181 gccggatatc aggaaagccg ttatagcttt acagccagag gtggttccta tatctacagt
     9241 tctgaggagg gattcagaga tgatatcggc tccttcccga atggagaaag ggcaatcggc
     9301 tacaaacaac gttttaaaat gccctacatt ggcttgactg gaagttatcg ttatgaagat
     9361 tttgaactcg gtggcacatt taaatacagc ggctgggtgg aatcatctga taacgatgaa
     9421 cactatgacc cgggaaaaag aatcacttat cgcagtaagg tcaaagacca aaattactat
     9481 tctgttgcag tcaatgcagg ttattacgtc acacctaacg caaaagttta tgttgaaggc
     9541 gcatggaatc gggttacgaa taaaaaaggt aatacttcac tttatgatca caataataat
     9601 acttcagact acagcaaaaa tggagcaggt atagaaaact ataacttcat cactactgct
     9661 ggtcttaagt acacatttta agaacgccaa ctaaaatttc cccgaggtga aaatcgcccc
     9721 ggggaataac tagccatttc aatg
//
LOCUS       DAPGWD010000158         9731 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPGWD010000158 DAPGWD010000000
VERSION     DAPGWD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.159"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            263..1576
                     /gene="gltP"
                     /locus_tag="RHX88_003697"
     CDS             263..1576
                     /gene="gltP"
                     /locus_tag="RHX88_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDV2232344.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1654..2343)
                     /locus_tag="RHX88_003698"
     CDS             complement(1654..2343)
                     /locus_tag="RHX88_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV2232345.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2437..4584)
                     /gene="fdhF"
                     /locus_tag="RHX88_003699"
     CDS             complement(2437..4584)
                     /gene="fdhF"
                     /locus_tag="RHX88_003699"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4165..4167),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDV2232346.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(4782..6248)
                     /gene="mdtP"
                     /locus_tag="RHX88_003700"
     CDS             complement(4782..6248)
                     /gene="mdtP"
                     /locus_tag="RHX88_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDV2232347.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(6245..8296)
                     /gene="mdtO"
                     /locus_tag="RHX88_003701"
     CDS             complement(6245..8296)
                     /gene="mdtO"
                     /locus_tag="RHX88_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDV2232348.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(8296..9327)
                     /gene="mdtN"
                     /locus_tag="RHX88_003702"
     CDS             complement(8296..9327)
                     /gene="mdtN"
                     /locus_tag="RHX88_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDV2232349.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPFEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRFGA
                     SAVANLEPQ"
     gene            complement(9346..9621)
                     /locus_tag="RHX88_003703"
     CDS             complement(9346..9621)
                     /locus_tag="RHX88_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232350.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         2201 a         2588 c         2603 g         2339 t
ORIGIN      
        1 agtattattg cagattaaac aaataaaaat ctttccataa caaatggtta ttcattaatc
       61 ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt
      121 gcgcaacata ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta caaaccaacg
      181 caacacaatt catgccctgg cagtatgtca cgttctcgcg tttctgaacg gggaacggcg
      241 ctccattgag gaagttattc tgatgaaaag tataaaattc agcctggcct ggcagattct
      301 gtttgctatg gtgctgggca ttctcctggg aagctacctg cactaccata gcgacagccg
      361 cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc atctgatcaa
      421 aatgattgtt gtgccgattg tgatctccac gctggtggtg ggtatcgcgg gtgttggtga
      481 tgccaaacag ctcgggcgta ttggcgcgaa aaccattatt tacttcgagg tgatcaccac
      541 cgtcgccatc attttgggga tcactctggc gaacgtcttc cagcccggtg ccggggtgga
      601 tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag agcactacgg aagcggtaca
      661 aagcagttcc cacggcatta tgggcacgat tttgtcgctg gtgccgacga acattgtggc
      721 gtcgatggcg aaaggcgaaa tgctgccgat catcttcttc tcggtgctgt ttggtctggg
      781 gctttcctcc ctgcccgcga cgcatcgtga accgctggtg accgtgttcc gctccatctc
      841 tgaaaccatg tttaaagtga ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc
      901 gctgattgcg gtgacggtgg ctaactttgg tttctcttct ctgtggccac tggcgaaact
      961 ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc
     1021 gcgcctgtgc gggttaagcg tctggatcct gattcgtatt ctgaaagatg agctgattct
     1081 ggcgtactcc actgccagct ctgaaagcgt actgccgcga attattgaga agatggaagc
     1141 ctacggagca ccggcgtcga tcaccagctt cgtggtgccg accggttact cttttaacct
     1201 tgatggttcg acgctgtatc aaagtattgc cgctattttc atcgctcagc tgtatggcat
     1261 tgacctgtcc atctggcagg aaatcattct ggtgctgacg ctgatggtga cctcgaaagg
     1321 gattgctggc gtgccgggcg tgtcgtttgt ggtgctgctg gcaacgctgg gtagcgtagg
     1381 tattccgctg gaaggcctgg cgtttattgc tggtgttgac cgtatcctcg acatggcgcg
     1441 tactgcgctg aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca
     1501 caaatttgac cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca aatttgataa
     1561 aactgcggat caataattga agattgccgg ggttatccac ccggcaatgt gtggatgcct
     1621 gatgcgacgc tggtgcgtct tatcaggcct accttaaccg ttggttaatt tttcaaactc
     1681 ttcacacccg gtatcaaacc cttccataca gctcaggttc aaccagtgca gcgccttttg
     1741 tttgttcttc tcgataaaac cttcctcacc gtttaagaac atcattcccg cccagtactc
     1801 ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc
     1861 gtcggcctta atgcccacgc cgttggcgta aatcaaacca agcagcattt gggcatcgac
     1921 ggcggagtca ttctccagat cttccgaggc gttttctaac agcgaaatcg cctttggata
     1981 atccggttta cccgcttgag tatttaccag aatatgcgcc agcgttactt caccttcttt
     2041 actgcccgct tgcgccgctt tctctgcaag aacttttgct tgtggatagt ccagactgac
     2101 cggattggtg attttgatct gcgccagcag cgcgcaggca tcagcatcac cgctgtcggc
     2161 ggctttctgt gcccaatatt cggctttgct caaatcgccg gagctaaacc agctgtcggc
     2221 aagaaaatat tgtgcgcgtc ggtccccggc ctctgctgcc tttaaatact ggctgccagg
     2281 ctcggagtcg ttcgcgtggg caaagaatgt caaaaacaac atcaatgcga taattttttt
     2341 cattttgata atacgattgg gtagggagta accagtataa agagttcgaa tggataaaaa
     2401 aacagcctcc gaaaggaggc tgtagaaagg actgtattac gccagtgccg cttcgcgcag
     2461 gcgagttttc aacttgttgt actcgtcaat cacgtactgc tcggcggcgc gctgatcggc
     2521 gatcggctcg acgcgcacgg cgcagtattt gtattccggc gttttcgtaa tcgggcttaa
     2581 gttttcggta accagctcgt tacaggcacc aatccaccac tggtaggtca tgtaaatcgc
     2641 ccctttgttc ggacgatcgc tgacctgcgc acgggtgata attttgcctt tacgcgagtg
     2701 cacccaaacc aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg tattgatttg
     2761 tgcgtagcca ggttcatcag ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg
     2821 gcaagagtag tgaccaactt cacgcaccgt tgacagtacc atcgggtact cgtcggtgag
     2881 tttgtcgatt ggcgctaccc agtcgcaggt gaagaactgt gccagaccgt tcggggtatc
     2941 aaacttctct ttaaacagat aagaagtccc ctgatcggca tctgaagtat cgcggcaagg
     3001 ccactgaatg aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca
     3061 cagatgacgc aactcatccc agatctcctg ggtgttgttg tagtgcatcg gataacccat
     3121 acgggtggcg atttcactga tgatttgcca gtccgttttc agatcccatt tcggttcaac
     3181 tgctttgaag aaacgctgga agccacggtc agccgcagta aacacgcctt catgctcgcc
     3241 ccacgacgtt gacggcaaaa tgacatccgc cgccgacgcg gttttggtca taaagatgtc
     3301 ctgaacgata accagttcca gatcttcaaa ggctttacgt actgccgaca gctccgcgtc
     3361 agtttgcagc ggatcttcgc ccataatgta cgcggcacgc acttcgccat gcgctgcgcg
     3421 gtgcggcagc tcgctgatgc gataaccggt atgagcaggc aggctttcca cgccccaggc
     3481 tttggcgaat ttctcgcggt tagccggatc tttcacgtac tggtatcccg gatacgtatc
     3541 cggcagcgcg cccatatcgc aggcaccctg cacgttgttc tgaccacgaa ccgggtttac
     3601 acccgcatgc ggcttaccga ggttaccggt cagcatcgcg aggctggtca gagaacgcac
     3661 ggtttccacg ccctggtaga actgggttac acccatgccc cacaggatgg cggcgctttt
     3721 cgcctgggca tacatccgtg ccgcctgacg aatctcactg gcgctgacgc cggtgatatc
     3781 ttcaaccgac tccggcgtgt agccttcaac gattttacga tactcttcaa agccttctgt
     3841 acgggaagcg acgaacgctt tgtcgtacag attttcttca ataatgacat ggcccatcgc
     3901 attcaacagc gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc
     3961 ggtttcaatt ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt tagcgttaat
     4021 tacgtgattc gccacgattg ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa
     4081 atcggtatta tcaatttcgt taatagcatt gctcattgcg ccattaccga ccgattggtg
     4141 cagacctgca accgatgggc cgtgtcagac acgagcgcag cagtcaacgt tattggtacc
     4201 aataacggcg cgcgcaaatt tttgcattac atagttggtt tcgttacccg taccacgcga
     4261 ggagccggtc gtctggatgg catccggacc gtacttctct ttgatggcgc tcaggcgctc
     4321 ggcaacgtaa ttcagtgcct catcccagga aacaggttcg agtttgccgc cacgctggcg
     4381 acggatcatg ggggttttca ggcgcggggt caggatctgg gtatcgttaa tgaaatccca
     4441 gccataataa cccttcagac acagggtacc ctggttggtt ttcccctgcg ctgcctccgc
     4501 ccggacgatt ttgccgttat cgacgaccag gttgattttg caacctgatg cgcaataggg
     4561 gcaaaccgtg acgacttttt tcatcggtct cgctccagtt aatcaaatca cgcatacgcg
     4621 ctctcgacta cagtatgcat cttttatgcc acattttatt tggggtcatt ccctgatatt
     4681 acgggcacta tttattcaaa actctgacga aaaacaggct gtcgtcagtt ttgacgtgac
     4741 gaaacgaaat accgcgtgac agccatcacg cggcagacat tttatttttt ctcgacgacg
     4801 ggagccgcct gatacccgcc gcccagcgat ttcatcaact gaatgctttg gatcacccgg
     4861 cggctgtcca gcatcagtaa tgacatctct tcggcaagta ctggcaaccg ggcttcagtg
     4921 gcctgtaagc ggctggttaa gccgcgctga taggcggcct cggcagcgcg ctgggtgaag
     4981 cgcgttgctt ccacgcgttc agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc
     5041 gtgccgttga cggcaacgtc acgcaccgcg ttcagtactg actggttgta acgttcaatc
     5101 atcatgttgc tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc accgtcaaac
     5161 agcggcaatt tcagacccgg gatgaagttg aactggcgac tggttttttt gaataaggta
     5221 tccaggtgga tggcatcaag gccgaaaaac gctttgatat caaagctcgg gtagaacagc
     5281 gcccgcgcgg aatccacctg atctaatgac gcctgaacat accagcgcat ggcttgcaga
     5341 tccgggcgtc tggcgagcaa ctcataagag agtgtcgccg gaatgccggt ctggactcgt
     5401 ggtaatgcca ccggtttgat ctccggcata tcgctggccc ccgcgccaat caatgcacgc
     5461 agagattctc gcgtttcggt gatttgccct ttgacggcag caatttgttt atcgaccgcc
     5521 agaatctgtg cccgcgcgcc gtggaaaggc acttgtgctt ccagaccgtg cgccacttta
     5581 ctctggtgcg ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa cagatcgagc
     5641 atctgatagc tggcctgcat actgtaataa agctgcgcta cgcccgtggt cagcgatagc
     5701 tctactgctg cggtttctgc cagcgcggca ttatgcgcgc caatggcggc ggcaaccgct
     5761 gagcgatgca caccccacaa atcaagatcc agtccggcaa acaaacctac tgtggcttcc
     5821 gtatagtacg gcccgtccat acccagtgcg ggcgcatcca tcgcataagg gcttaaaaag
     5881 ccgttcgccg agacgcgttg gcggttgagc atccctaacg ctgcgacctg taattgtgaa
     5941 ccggcatcta acaaatcggc ctgggactgc gctttttctt cccgcagttt cgcttcggcg
     6001 agggtgtgtg aaccacttag cgtccgttgg atcagcgcat ccagctgcgg gtcattgagt
     6061 tgtttccacc actgcgcctg cggccagccg gagctggcaa gatgaatatc gtcggccagt
     6121 ttgatttgtt ccggtttgag ctgttgatga ggtgcagaat ccttacgtac cagggcacag
     6181 ccggaaatca gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga aagttgacga
     6241 ttgatcattg cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc
     6301 agtctggcaa gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat aaggtgggca
     6361 ggatggcctg cgaggcgggt gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg
     6421 ttgccagacc ggcagcgtat ttctccaggg cgtcggcaaa ctgagctgct ctgtccggct
     6481 gtagtgcgtt atccagcgcc tgcgccgagt tccaggtggc atcccaggcg tgaagaatat
     6541 cgcggccctg acgaataacc gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt
     6601 ccagttgacg ctccagcgcc acgcgttggc acatctcttc acaggcatta aacgccgcat
     6661 gcagaccgat acgaatttgc agataagtgc gcagagccgt gacttcctgc tggcgtggga
     6721 tccgcattac tttacttaac atacccagcg tgccagccag tttttgcggc agtgtgcgcg
     6781 cttcactttc aggccagaca aaggtgtaaa tcaccgcgga caccacggta ccaatgatga
     6841 tacccatggc gcgatcgcga atttccacca ggtcgtacac cgggccaaaa acgttttcga
     6901 gcgtggcgag cgcgaaggtg accaccatct gtgtgccgat ataagaagag cgttcagagc
     6961 tggtggcaat ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc aattcgacaa
     7021 tattgtccag ccagggcatg accagtagcg tgaataacag cgccagaatc gcgccgcaaa
     7081 aggccccgcc aaaacgcagc accatcttct ggtacgacga accgacattt gggttagcga
     7141 cgatcacgca tgtcagcata caggtgtgaa tgccttccca gtccacgccg ctgtagaagg
     7201 tgtaacagat caaacaggcg agcagtgttt ttaccgcgta gcgcatatag tctggattgg
     7261 taaaagcatc ggcgaccatt gatggcggtt tggcggcggg cgttggcggc gtattcgggt
     7321 ccatctggcc cagttgtaac aacgtctggc agatattctc caggttacat tcccgtgccg
     7381 ccatcgcttc actttcagtg atccgccagt cgctttgcca gcactgacct tcagcaacgg
     7441 catgctgcag cttattgatt tctgaggcta atttttgccg aaattcaata attgcctgag
     7501 aatcagcaaa agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg
     7561 ccacgcagct ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa
     7621 agacattcag tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg
     7681 cgaggctgtc cgtcaggtga ctaatggcat catcaagccg atcattaagc gcttgatgca
     7741 tttgcgtaat ggcacgactg ggaaaccaca gcacgccgat taacgtcatc agcaaggttg
     7801 gatagaggcc aacaacgata caccacagcg ttaagcgcac gaccacttcc ggatagtcga
     7861 gcatggcggg gaaggtttgc ccgtaaatgg cgacaatggc gacagcgaaa aagaccagtc
     7921 ccagccgatg ggtgcgcatc aaaaacatac agcccatcag gatcggcccg gcgatgatca
     7981 atcggatcaa cggttcgccg tatgaccatt tgtagatcaa aaacaggctg ccgatctcca
     8041 gcaccgtggc aaccacaaac aagatcgcga caaatttggt gtaaaacgcg ttcgactgaa
     8101 taccgtaaaa cagcactgcc agcgataacg ccacaaaagg gatctcaaaa gtcatcgaga
     8161 tcagaatcac cagcaggcag cctacccaga gttgcacgat ctgcggcacg cggcctggtc
     8221 gccgctcgct taactcttca tgaaagaacg ccagcagcct gaccaccggt aatggcaggg
     8281 agttgagcgc gctcattatt gcggctcaag attagcgact gccgaagcgc cgaagcggaa
     8341 catttccggg tcaggtttat cgaccatgat tttgaccgga aaacgctggg caacgcggac
     8401 ccagttaata gagcgagaaa ctttcggcag gccgcccagc accaggccgc cgtcatccgg
     8461 tagcacgccg tagccaatcg aatccacttt accctcgaag gttttgccgc tgtcactcat
     8521 caggcgaatc gttgcgggtg tacctgagcg aatatttttc agatcggttt cgcggaagtt
     8581 ggcgatcacg taccagtgac gagtgtcgat tagggtaaaa atagggcgca tggcagaagc
     8641 aaattgcccg acggaggttt tgagggaaat gacccggcca tcaaacggcg cgcgaacggt
     8701 cgccatttcc agatgaagtt tggtcagggc aatatccgct tcgaccgccg cacgttgcgc
     8761 caccagcgca tcgacgccgc tgacggcgct ggcggctgac tgcgcctgta acaatacggc
     8821 attaagatcg gcttctgcgg cgcgctgcgc agttcttgca cggtcaacgt cttccgctga
     8881 gacaaaaccc tctttcagta atggctcggt gcggcgtaat gtatctgtgg cctgtttcgc
     8941 ggcggcacgg gctttttcta ccgtggcatt aatcgagtcg gcaccaaact gttgcgcatc
     9001 aacgctacgc tgggtgagca taatttgctt atccagcgcc gcgagggagg cttcagcttt
     9061 cgccagattg gcttcgaacg ggcgcgggtc aatgcggaac agcaaatcgc cctgtttgac
     9121 tgcctggttg tcggtgaccg ccagttctac aatgcggccg ctgacttccg gcaccacatc
     9181 aatggtatct gctgacgcgt aagcgtcatt agttgatggc gcactgtcta cgcgccagat
     9241 aacgaaaaca agggcaacca gcgccaatgc aaccactaac agagcaggga atttactgcg
     9301 aggagctttt ttcggcgtac tttccatgag cgcgtctcaa atagattaga aaaatgccag
     9361 ccagaacagc atggcgtaga gagcaaaaag ggcggtataa ataattccga caaatgccag
     9421 attgatattt gcccgctgaa taactcgcct cgtgatgagc atcaaaataa ggctggcaat
     9481 ggcgcagaaa aaccagctgg gataataagc accgatcacc gggattgccg gagataatga
     9541 gcatcccgaa accatgaaga cgggaattat ccaggctgtt tttttgagtt gcatcgccat
     9601 gcgagagaga actgttggca tattaaatac atcctgtctg ctgcgaacaa tatctgtagt
     9661 gaatgtcaat atccattgaa ggcgtcagta tggctatttt ctcgatgaat aaaaatagct
     9721 ttattcagcc a
//
LOCUS       DAPGWD010000159         9692 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPGWD010000159 DAPGWD010000000
VERSION     DAPGWD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.160"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(222..980)
                     /gene="chbG"
                     /locus_tag="RHX88_003704"
     CDS             complement(222..980)
                     /gene="chbG"
                     /locus_tag="RHX88_003704"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDV2232351.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(993..2345)
                     /gene="celF"
                     /locus_tag="RHX88_003705"
                     /gene_synonym="chbF"
     CDS             complement(993..2345)
                     /gene="celF"
                     /locus_tag="RHX88_003705"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV2232352.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(2450..3292)
                     /gene="chbR"
                     /locus_tag="RHX88_003706"
     CDS             complement(2450..3292)
                     /gene="chbR"
                     /locus_tag="RHX88_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDV2232353.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(3300..3650)
                     /gene="chbA"
                     /locus_tag="RHX88_003707"
     CDS             complement(3300..3650)
                     /gene="chbA"
                     /locus_tag="RHX88_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDV2232354.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(3701..5059)
                     /gene="chbC"
                     /locus_tag="RHX88_003708"
     CDS             complement(3701..5059)
                     /gene="chbC"
                     /locus_tag="RHX88_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDV2232355.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(5144..5464)
                     /gene="chbB"
                     /locus_tag="RHX88_003709"
     CDS             complement(5144..5464)
                     /gene="chbB"
                     /locus_tag="RHX88_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDV2232356.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(5763..6101)
                     /gene="osmE"
                     /locus_tag="RHX88_003710"
     CDS             complement(5763..6101)
                     /gene="osmE"
                     /locus_tag="RHX88_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDV2232357.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            6303..7130
                     /gene="nadE"
                     /locus_tag="RHX88_003711"
     CDS             6303..7130
                     /gene="nadE"
                     /locus_tag="RHX88_003711"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDV2232358.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            7360..8247
                     /gene="cho"
                     /locus_tag="RHX88_003712"
     CDS             7360..8247
                     /gene="cho"
                     /locus_tag="RHX88_003712"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDV2232359.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(8207..8782)
                     /gene="ves"
                     /locus_tag="RHX88_003713"
     CDS             complement(8207..8782)
                     /gene="ves"
                     /locus_tag="RHX88_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDV2232360.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(8985..9470)
                     /gene="spy"
                     /locus_tag="RHX88_003714"
     CDS             complement(8985..9470)
                     /gene="spy"
                     /locus_tag="RHX88_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDV2232361.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         2555 a         2440 c         2195 g         2502 t
ORIGIN      
        1 cgcaacaggt tagaacccaa ggcccgatac ggcatgcgga ctgcaggcag agaaaactgg
       61 ccgattttct ctgcactgga gggataatac aaggcctgat aagcgaagcg catcaggcag
      121 ttttgcgttt gtcagcagtc tcaagcggcg cagttacgcc gcctttgtag gaattaatcg
      181 ccggatgcaa ggttcacgcc gcatctggca aacatcctca cttacacatc ccgataactc
      241 cccaaccgat aaccacgctg agcgatagca cctttcaacg acgctgatgt caacacatcc
      301 agctccgtta agcgtgggaa acagtaagca ctctgacgga tagtattatc gataaacgcc
      361 ggatgacaca tcacctccag cgaacgatca ccccgatggc ttgcgtcatc cagcacttgc
      421 aggaacagtg cctcgctaat ctcttcacca taaaacgcgc tactgaatcc ctgagaactg
      481 cgcagattag ccggtaaatc accagcgtta gatagcggct gacggtcgat gcgcaacgca
      541 atcccctctt cagccgcaaa cctggcaaca atcgggaaaa tctgcgggaa catatgcaca
      601 tgatgatggc tatcaagatg cgtaggtttg cgtccaaata gctcaatgaa acgcaaatac
      661 tgactggcaa gctcctgagt aatttcttcc agcggtaaag catcttcttc tgccaactgc
      721 cagatccatt ttcccagcac accatcgcgg gttaaccccg gcatagctgt cagtggcttg
      781 cccatagtaa ggacaaagtg catccctatg gccagactcg gttcatcacg actcaactgc
      841 accgcatggt caatagcctg cccattcacc agcgccgtcg ttgatgtgac aatcccattg
      901 cgacaggcct cgataatgcc gtagttctga cctttgctta agccaaaatc atcggcatta
      961 acaatcagta agcgttccat aatcagcctc ggttaatgtg cttttttaag ctctgcgatg
     1021 cagtcggcaa aatttggcag ccatttctcg tgcgccagaa tcatctcgcg tgccagcagc
     1081 tcagcatcgc gatcggaatg caccaacgga ctaaggttta gcgccagtaa cacatcgtta
     1141 aattctccgc taagtgcggc gttgctggca gcaatctcga agcctttaat ggtgtgaatc
     1201 agccccatca ctttatcatc gaaatgcgta atgcgcggat gtggcgtcgc gccatcgcgc
     1261 cccagcttac aggtcatttc taccgcccag tctgccggaa tattatcaat atgcccatga
     1321 tgcgggatat taacgtaatg ttcagcttgc ttgtcgttgt agatagcgtt gatcacttcg
     1381 cacgctgcat cagagtaata agccccaccg cgctgttcca gttctttcgg cttaactttc
     1441 aactccggat ttttatacag ctcaaaaagt tgtttctcga ctttctgtac tacctgtgct
     1501 cgtgcgccgc ctttgtagta ttcgcccatt tcaatagcca gcatctcttt ctgcttgaag
     1561 taatacagca gataagaaca tggcagcaga tttaacgaac gaattaagcc ctcactaaat
     1621 ggcagatcga aaatattttt tacagaggac gcttttaact gccctgacgc cacaccatca
     1681 agcaattcgg caaagcgcga cttgccattt accagcacat ccttaatgaa caccatatgg
     1741 ttgaggccga acagatcgat agataaatca tcgctgtctt tcagcatcag aacatcgcga
     1801 ataaacatct tcatgccgat cggaatatta cacacgccga taaagcgttt aaatccggta
     1861 tgacgataaa cggcttcagt gaccattccc gccgggttag tgaagttaat cacccatgca
     1921 ttcggacaaa gttcttcgac atcttttacg atgtcaaaaa tcaccggaat ggtacgcaga
     1981 cctttaaaca gaccgcccgc gccgttggtt tcctgaccaa gataaccatg acttaatgga
     2041 atacgttcat ccagttcgcg cgccggtaat tggcctacgc gcagttgggt agtaacgaaa
     2101 tcagcatctt tcaatgcttc gcggcgatcc agcgttttat aaagcttcat cgggacgcca
     2161 gcgttatcaa tcatccgttg gcagagttca aaaatgatat ccagtttctc tttaccatct
     2221 tcgacatcca ccagccataa ttcgctgacc ggcaattcgt gataacgctt aataaatcct
     2281 tccagtaact ccggggtata gctgctcccg ccaccaatag tgacgacttt taatttctgg
     2341 ctcataattt ctcccttcag tacagaatac tgatatctgg catatctgcc cccccggaca
     2401 taaataatcc agcaacagta cagatatgtg aattgtcagg tataacgact tactgattaa
     2461 attcagtcaa tttcttacga tagctcttag gcgtaaagga cgttaatttc ttaaacgttt
     2521 taataaacaa actcgggcta ctataaccgg cctcaaacgc aatatccgtc acggaatagt
     2581 tggtcatttc cagttgtttt ttggcaaaat taatacggat ctcattaata atctgcattg
     2641 gtgttttgcc ataatatcgt tgagtcgctc gcgtcaaata ttcctgtgat ttggctgaca
     2701 acgtcaccat attctccagc gccgattcac taaactgctc tttatcatgc atcttttcta
     2761 ccgtactttt cagccactgc ggtacatcat caatcacctg ttcttcgcgg taatgacgta
     2821 aacggttaat gacataaaaa gtaaccatct caacaaactc ttccagccct gtttcgcgga
     2881 aattcaatga agaaatcact gtttccacat aggtgagaaa agcgttattg gtccggtata
     2941 cctgcgaagc gacaaagcaa taaggcaaca acggcaggta atgctgctca aaaaagcgtt
     3001 tactgatccc aacgttcaat atacgcgtgg caccaaactc ataaaaactt tgatggtgcg
     3061 aacctaacgg aataaaaaca aaatcgcccc gttccagtaa cacgcgctta ccgttaatct
     3121 cctggaaata acgcccggtt aataccagag taaattcata atagtcgtgc tgatgcagcc
     3181 cgctgatact ctcagttttg ttatagataa acacatggaa gtttttgcca ttaaacaact
     3241 gctgttctcg ggcagtggca atttccggcg cgttaatcac tggctgcatc atcgactcct
     3301 tatgccttca gtttttcatg aagctcaatt aattcagtaa tcagttcacg cgcaagcatg
     3361 gacgtcatta aatgatcctg agcgtggacc agcaccagac tcactttcat cttaccttcg
     3421 cccgcatcgc cttcaatcag tttcgtctgt accagatgcg cttcattcaa tgccatccgt
     3481 gactgatcca tcatggcttt tgctgcagca aaatcgccct gcttcgcctg tttcagtgcc
     3541 gcatacgcca ggctgcgcgc ttgtccggag ttgatgataa gccccatcac cacttcttcc
     3601 agctcttcag cttccgtttg cgtatcggga atgttatcga gatccatcat acatcgttcc
     3661 tctctattct taccggcacg attacccgta ccggcatcga ttaaaatttc agggcgttag
     3721 cgatatcttc ttcgctctct tctttatcaa tcgcgttttg tgctttgtta gccaccacaa
     3781 caaagggcag ataaattaac gttgcgatac caaggttgaa gagtgcgacc agcaatgcgg
     3841 cgacgctacc gttggtgtta aagaaggctc ccagaccggt tggcatggtc cacggtgcaa
     3901 tattggtcac cggaggaatg atgcccatgt agtacgctgc gagggtgatt gccgccagaa
     3961 tcggttgtac cagtacaaac gggataaaca tcaccgggtt catgataatt ggcagaccaa
     4021 acagaatcgg ttcgttaatc tggaagatgc cggacggcag cgccagtttt gccacctgac
     4081 gataatcagc acggcgagag gcgataaaga tagccaggat caggcctaaa gtcgcaccac
     4141 tgcccccaag gaaaataaag gagtccagca tcggcttggc ccagatatgg aaggtcttac
     4201 cggctgccag cgccgcttcg acggaaccat attgctgata ggtcgcgata ttttccagcg
     4261 cccacggcgt cataatgccg ttgtccagtg cggtcagcgc cagcgcgcca tgaataccga
     4321 agaaccagag cagtggaaca aagatcacat aggcccagcc caccacgcta cccaacgatg
     4381 ccagtggggt tgagatggta tccataatga tctgatggaa gttggtgccc caggtattca
     4441 acgcccaggc aataatcccc atcacggaaa gaataataaa gccgggaatt aatgccgaga
     4501 aggaacgcga tactgaagca ggtacgctat cgggcaattt aatgacccaa ttgcggcgga
     4561 caataaaggt aaacatttct gccaccacca ggccaataat aatcccggag atgatattcg
     4621 ctccacctaa ccagtttgcg ccaaccgcat aggcctcacc gacactatat ggggtgacgg
     4681 tcataaatgc tgcaacggat aacaacccag ccgccagcgc atcgacttta cgctcttctg
     4741 ccagcgccat gccaataaag aacggtgcca ttaaagacat tatccctaat gttccgttat
     4801 atacgttgcc gccaatacct ttcagaccat taagtgtttc aatggttgag gcgtcgaggc
     4861 gaatacctaa ggaataaaaa aacgacccct ccccaaagct tagaaaaacg ttgttaatta
     4921 atacaaacat ggcccccgca agggttaacg gcattaagcg aataaagcca tttttgattg
     4981 cattaacgtg tggctgcttt cctattttaa ctgcaaaagg gaggagtacc ttttcaagcg
     5041 atgcaataac attactcata gaaaaatacc cttaaaaacc gcaatttaaa tattgcggta
     5101 ttgatttatg aaataactct ttgacgggaa aatttaaaat aaattaattt gctgcggctt
     5161 ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc atcgactttg ccataaagca
     5221 gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg ctggatttcg ggcaacatat
     5281 aagcaatctg cggccctaat aacacgacat cggcattctg acctttttca ccagccagtg
     5341 tttccggaaa tgcttcaata atgaccggaa cttcatattt ttctgcctgt gcgcgcattt
     5401 ttgataccag taaagaggta gacatgcccg cagaacaaaa cagataaatg tgtttctttt
     5461 ccataaaact gccctcatcg acgattatct gtcagccaga cactccgcaa gccttaacct
     5521 gcttccatgc tctgggtaac ttgcgaaacc aacatgactt tttttgtggc tgaaaggagt
     5581 atacggtata gacctgagca atagtatttc tttgacctta taaattgttt ctccttgacc
     5641 tgtggttatg accctcttca catttcgggc aaataattcg cgaagataat taagaactca
     5701 ggccaaaaaa aaccggcgca atggccggtt tccgttgtta ctcaagcttt cagacgaatt
     5761 gattacttcg cagcctgtgg atcagtgtcg tattcagcac aggtctgata accggagttg
     5821 atgacatgtc cggtatcatc taacgcgaca aagtaggttt ctgctttacc atcacgttga
     5881 cccaggatgt aggtctggca agtaccgcga gcatggatca tgctcacttc agacgaaggt
     5941 ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt ttttgacgtc tttcaccaca
     6001 ggctgtacaa actggtcttt ggtacgatca taagccgtac aacccgccag catggttaat
     6061 accgccgctg cactcagaat tcctgccata ttcttgttca tattccgtcc tcttgtttat
     6121 cagcgtgtta gataagcctg gaatacattg ggcgcttttt caagcccgtg aacgaaacgg
     6181 ctccgctttc agaggattcc tgtatgacgt tttaaccacc attcagcccg ctgtcgcttg
     6241 tcgtttcagt agcaacgggt tagctttaag gaagttttgt cttttctgtc tggaggggtt
     6301 caatgacatt gcaacaacaa ataataaagg cgctgggcgc aaaaccgcag attaatgctg
     6361 aagaggaaat tcgtcgtagt gtcgattttc tgaaaagcta cctgcaaact tatccgttca
     6421 ttaaatcact ggtgctcggg atcagcggcg gtcaggactc cacgcttgcc ggaaagctgt
     6481 gccagatggc gattaatgag ctgcgccagg aaactggcaa cgaatcactg caatttattg
     6541 ccgtacgcct gccctatggt gttcaggccg acgaacaaga ttgccaggat gccattgcct
     6601 ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg cgcggtattg gctagcgagc
     6661 aggcattgcg ggaagcaggc attgaactga gcgattttgt ccgtggcaat gaaaaagcgc
     6721 gtgagcggat gaaagcacaa tatagcattg cgggtatgac cagcggtgtc gtggtgggca
     6781 ccgatcatgc agcagaagcc attaccggat tcttcactaa atatggtgac ggcggtacgg
     6841 acattaatcc gctgtatcgt ctcaacaaac gtcagggtaa acagttactg gcggcattag
     6901 gttgcccgga acacctttat aagaaagcgc caacggccga tctggaagat gatcgccctt
     6961 ctctgccaga tgaagtggca ctcggcgtga cctatgacaa tatcgacgac tatctggaag
     7021 ggaaaaacgt acctgaacag gtcgccagaa caatagagaa ctggtatctg aaaaccgaac
     7081 ataaacgccg tccgccaatt accgttttcg atgatttctg gaaaaagtaa taatttgcac
     7141 atattggatt gtgcgaaaaa gagtaatttg ttcacgccgg atgcggcgtg aacgccttat
     7201 tcgacctaca aaactatgca aattcaatac attgcaggag tcgaataggc ctgacaggcg
     7261 tagcacgtca gacggtgtaa cctttgtcat cgacccgcct cttttttaat cgcttcccgg
     7321 ctgttacact ggatagataa ccagcattcg gagtcaacag tggtacggcg tttaacttct
     7381 ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc ccgaacattt acgttcattc
     7441 cttaatgact tacccacccg accaggggtg tatctgtttc atggcgaaag tgacaccatg
     7501 ccgctctata tcggcaaaag cgttaatatc cgcagtcggg tgctttctca tttacgcacc
     7561 ccggatgaag ccgccatgct acggcaatcc cggcgaatta gctggatctg caccgcaggc
     7621 gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag aacaacagcc gctgtttaat
     7681 aaacggttgc gccgtaatcg ccagctctgt gccctgcaat taaatgaaaa gcgcgtcgat
     7741 gtggtgtatg ccaaagaggt ggatttttca cgagccccca acctgtttgg cctgtttgcc
     7801 aataggcgcg cagctttgca agcattgcag agcatcgctg atgaacaaaa actttgttat
     7861 ggcctgttgg gactggaacc gttaagtcgc ggtcgtgcgt gttttcgttc agcgctaaaa
     7921 cgttgcgccg gagcatgctg cggtaaagag agccatgagg aacatgcgct acgcttgcgc
     7981 cagtctctgg agcgtttgcg ggtggtgtgt tggccttggc aaggtgcggt ggcactgaaa
     8041 gaacagcacc cggaaatgac tcaatatcat attattcaaa actggctgtg gctgggggcg
     8101 gttaattcgc tggaagaagc gacaacgtta attcggacac ccgccgggtt tgatcacgac
     8161 ggttataaaa ttctttgtaa gccgctgctt tccggtaact atgaaattac tgaacttgat
     8221 ccggcgaatg accagcgagc cagttgattt cactgaacaa cagtttacct tgcggttgta
     8281 aaagccgcag ggtgtgcctg ccatcgaacc agcacgcgcc ctggtcggta gtgagcagtt
     8341 tgtcgcccag ttgccacgcc ccgttaatca caaacaccac cccgccgcgt gagccaaagg
     8401 tagtaaacgt acgttcggca attctgactt tcgccttaca gacatccagg cgtgtcatga
     8461 tgttgaaatc catcgacatc tgccctgccg tcagtttcgc ttttaccacc tggtccgctg
     8521 caaaggcaaa aggctgtaac ggttttaagg tatggttaaa gcggtctgcg ctttcaagga
     8581 gcatctcgcc gccttccagc aacgtcacta tcctttccat gccgggaaac aaagaaaact
     8641 ccccattcgc cgcgatggat gcaatgctgg cacgccagta aaaatcacgt tttgccggtg
     8701 ggaaagtgca aatttcgcgc gtttcaccgg cagcatttcg ccacagattc accgacattt
     8761 tacgcatatc aaagtattcc atactcgctc ccggcccgtt cttttcattg ttatcgttca
     8821 ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg ttagtggtga tcaggctttt
     8881 tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa
     8941 ggtagtggac aagaccggcg gtcttaagtt ttttggctga aagattattc agcagttgca
     9001 ggcattttac cttttgccgc tggacgttct gtcagacgct tctcaaaatt agcattaaat
     9061 tgctttttct gttccggcgt caggatgttg taaattttgt tctgggtttc catgtgcgcc
     9121 agcatgttag ctttgcgctg ttcttccatt tttgcgatct gcgcttcagc ttttgcttta
     9181 tcgaaggtat cgctggcaat gatgtcatgc attgcgcggc gttcttccag cggcggacgt
     9241 ttcatctggt cacgctggcc tttcatgatt tcgcggatct gctgtttctg cgcgtcggtc
     9301 aggttcaggt ctttgaacat catgtcctga tgcggaccga acttgccttt gtggtgcatc
     9361 atcggcttcg cgtcagccgg tgctgcggta gtggtgtctg cggcatgggc caggttagcc
     9421 gcgccaagag ccagggtaga ggcaacaaac agtgcagtta atttacgcat attctatatc
     9481 cttcctttca gttatttatt acggctttct taagtagcgt gccgtgttga cgagattaac
     9541 tttactgact ttagcgtcaa ttaatcagag caacggtaaa acaatgaaag tgtaaaaaac
     9601 acttttgcgc caattatgga gaaaaaaaga aaatttgatg gagagtgatg agagaataat
     9661 acaacacgat gattttgcag agattatgaa ga
//
LOCUS       DAPGWD010000160         9637 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPGWD010000160 DAPGWD010000000
VERSION     DAPGWD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.161"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(145..220)
                     /locus_tag="RHX88_003715"
     tRNA            complement(145..220)
                     /locus_tag="RHX88_003715"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Lys,seq:ttt)
     gene            complement(385..1176)
                     /gene="cpoB"
                     /locus_tag="RHX88_003716"
     CDS             complement(385..1176)
                     /gene="cpoB"
                     /locus_tag="RHX88_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDV2232362.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1186..1707)
                     /gene="pal"
                     /locus_tag="RHX88_003717"
     CDS             complement(1186..1707)
                     /gene="pal"
                     /locus_tag="RHX88_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDV2232363.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1742..3034)
                     /gene="tolB"
                     /locus_tag="RHX88_003718"
     CDS             complement(1742..3034)
                     /gene="tolB"
                     /locus_tag="RHX88_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDV2232364.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3167..4432)
                     /gene="tolA"
                     /locus_tag="RHX88_003719"
     CDS             complement(3167..4432)
                     /gene="tolA"
                     /locus_tag="RHX88_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV2232365.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4497..4925)
                     /gene="tolR"
                     /locus_tag="RHX88_003720"
     CDS             complement(4497..4925)
                     /gene="tolR"
                     /locus_tag="RHX88_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDV2232366.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDAIESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4929..5621)
                     /gene="tolQ"
                     /locus_tag="RHX88_003721"
     CDS             complement(4929..5621)
                     /gene="tolQ"
                     /locus_tag="RHX88_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDV2232367.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5618..6022)
                     /gene="ybgC"
                     /locus_tag="RHX88_003722"
     CDS             complement(5618..6022)
                     /gene="ybgC"
                     /locus_tag="RHX88_003722"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV2232368.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6172..6465)
                     /gene="ybgE"
                     /locus_tag="RHX88_003723"
     CDS             complement(6172..6465)
                     /gene="ybgE"
                     /locus_tag="RHX88_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDV2232369.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6465..6578)
                     /gene="cydX"
                     /locus_tag="RHX88_003724"
     CDS             complement(6465..6578)
                     /gene="cydX"
                     /locus_tag="RHX88_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDV2232370.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6593..7732)
                     /gene="cydB"
                     /locus_tag="RHX88_003725"
     CDS             complement(6593..7732)
                     /gene="cydB"
                     /locus_tag="RHX88_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV2232371.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7748..9316)
                     /gene="cydA"
                     /locus_tag="RHX88_003726"
     CDS             complement(7748..9316)
                     /gene="cydA"
                     /locus_tag="RHX88_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDV2232372.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2365 a         2620 c         2291 g         2361 t
ORIGIN      
        1 taatcaccca atactacgct ggatactaca actaactatg ttagtggtgg gtcgtgcagg
       61 atgactcgat ttcgtctcgc ccttcgggcc gttgctgatg caacgttatc cttcacgttt
      121 tactaccgct tccaccttag cgattggtgg gtcgtgcagg attcgaacct gcgaccaatt
      181 gattaaaagt caactgctct accaactgag ctaacgaccc actttttcgt tgctttcggt
      241 ttgtttgata tcccgtggca acggcggcat atattactga tttcagactt gagcgcaaca
      301 aaaatttcga tgcagatcac tcaactgctt atgattcgca cgacacgacc agaaataatg
      361 cgacttctgg tcgtgtgtta tgcattacat cgcgttcaga cgtttttgtg cctgtttagc
      421 gccatcagta ccagggtatt tactgataac ctgctggtat acggctttcg cttttgcggt
      481 gtcacctttg tcctgcatga tgacgccgac tttaaacatc gcatcagcag cctttggtga
      541 cttcggatag tttttcacta ccgaagcaaa atagtacgcc gcatcatctt ttttaccctt
      601 gttgtagttt aactgaccca gccaataatt ggcgtttggc aggtaagttg aatcagggta
      661 atttttgatg aaattctgaa atgccaccat tgcgtcatcc tggcgggatt tatcctgcac
      721 cagcgcaata gctgcattgt aatccgtgtt tgcatcgccg cttttcaccg gcgcgccagc
      781 attcgcagta ccagcatcag ctgtcggcgt cgttgatgcc gccgcaccgc tttgatcgct
      841 gctggttgat tgcgccgctg caccaccgct gctgaggctg tcgatctgca acaggatctg
      901 cttttgccgc tccacgacct gattcagttg atactgattt tcctgaatct gaccacgcag
      961 ggaatcaata tcggattgat tatcagaaag ttgttgctgg agttgggtta aaagctggct
     1021 gtgagcatta gaaatacgct caagttgagt gacgcggtct tcgaccgagc ctgagccgac
     1081 actactgatt ggtgcctgag caaaagcggc ccagggggcc gctataccaa ccagtaacga
     1141 cagactcaat agttgatgtc tgaagttact gctcatgcaa ttctcttagt aaaccagtac
     1201 cgcacgacgg tttttggagt atgccgcttc gtcatgaccc agtactgcag gtttttcttt
     1261 accgtaagaa acgatggaga tctggtctgc agaaacgcct ttaccctgca ggtacatctt
     1321 aacggcgttc gcacgacgtt cacccaggga gatgttgtat tccggagtac cacgttcgtc
     1381 cgcgtgacct tctacggtga ctttgtaaga cgggttgcta cgcaggaagt ttgcatgtgc
     1441 atccagcatt tgagcgaagt cagaacggat atcgtacttg tccagatcga agtaaacgat
     1501 gttgttctgc tgcagctgtt gcatttgcag acgagcctgc tcttcggaag acatgttgcc
     1561 gttgccgccg ttcgcatcca taccagtgcc ggcacccagc atgccttcgc tgccgtcatt
     1621 gctggcgttc ttgttggaag aacatgccgc aattgccata acaggcagag caatcatcag
     1681 ccctttcagc actttgttca gttgcatttc aatgattcct ttactattca attaattatt
     1741 atcacagata cggcgaccag gcagggaatt tgacctgtcc atcagttgcc ggaagacgcg
     1801 ctttgaaacg cccatctgta gaaaccaaat tcagcacgga tcccatcccc tgagaagagc
     1861 tgtagattac catagtgccg ttaggtgcca gacttggcgt ttcatccagg aacgtggacg
     1921 acagaacttg tacgcctccc gttgccagat cttgtttggc aatgtgctgc tgcccaccat
     1981 tggagctgac cattaccata aatttaccgt cgctgctgac atccgcatcc tggttctgcg
     2041 aaccttccca ggtaatacgt tgtggcgcac cgccgttgat attcacttta taaacctgcg
     2101 gacgaccggc ctggtcagaa gtaaatgcca ggttctggct atccgggaac caggtcggtt
     2161 cggtattgtt actgcgacca tcagtcacct ggcggatctg accagaagcc aaatccatta
     2221 cgtacaggtt cagactaccg gttttcgaca aggcgaatgc cagtttgctg ccgtctggcg
     2281 agaatgcagg tgcaccgttg tgacgcggga atgaagccac ctgacgtaca gcgccatttg
     2341 ccagcgtctg aataaccagc gcggaacgac cgctttcgaa ggtcacataa gccagtttag
     2401 aaccgtctgg tgaccacgcc ggagacatca gcggctgcgg tgaacggtga acgacaaact
     2461 ggttgtaacc gtcatagtca gatacgcgca gttcatacgg gaactgaccg ccgttggtct
     2521 gaacaacgta ggcaatacga gtacggaacg cacctttaat gccggtcagt ttttcaaaca
     2581 cttcatcact ggcggtatga ccagcataac gcagccactg cttgttcact ttgtacgagt
     2641 tctgagcaag tacagtaccc ggtgcgccgc cagtgtcaac aagttgataa gcaacattgt
     2701 aagaaccatc cggattcgga gtgacctgac cgacaactac agcgtcaatg cccagtgcgg
     2761 accatgcagc tggttgtact tcctgcgcac tacccggctg ctgtggcaga cgagcgcgat
     2821 ctaacggatt aaatttaccg ctgttacgca agtcagcagc aacgatgccg ccaatatctt
     2881 caggtgccgc accaggcccc gcccactgga aaggaacaac accaatagga cgaccggaat
     2941 ctacaccgct gtcgatcaca atgcggactt cagcatgcag aactgatgcc cacagtatga
     3001 gaaaaccaaa tgctactcgt aatgcctgct tcatcatatc tcccttatct ggaccgatgg
     3061 cccacgataa tttagcagaa tgttaacaaa ctaaaataca caaaactacc agaaccccgt
     3121 ggcaaccggt gcctgatgtt gaccgtccga acagtcaaca tcgcgattac ggtttgaagt
     3181 ccaatggcgc gtttttgaac acttcatata ctgcctggct tggtggtttc gggatcttcg
     3241 caagtttagc tgctgccaac gcagcctgac aaagtgcggg atcgccacct tcaggtttga
     3301 tatccagtaa cataccatcg ggtgccagtt ttatgcgcag cgtacaggtt ttgcctgcat
     3361 aggacgatgc gtcatagaac ttactttcga tagcagattt aatctgcccg gcatagttat
     3421 tgatatcggc ccctgatgcg ccattgtttt tagtattacc actcccggca ggcgaagcat
     3481 tgttcccttt cgcccctccc cccgttttcg gtgcattctt accagagctt agctcaccga
     3541 aaatatcatc tgcctctgcg gccgcttttg ctgcagcggc tttttcagct gcagcttttg
     3601 ccgctgccgc ttttttgtct gcggcggctt tttctgccgc tgctttctta tcagctgctg
     3661 ccttttcagc agccgctttt tctgctgctt ttttgtcggc tgcagctttc tctgctgccg
     3721 ctttcttatc agctgcagcc ttttcagcag ccgctttctt ctcagcttct gctttggctt
     3781 tttcagcagc ttcagttgcc gctttctttc ttgcttcagc tgcagctgct tctgccgctt
     3841 ccgctttctt cttcagtgcc gcagcggctg cctcggcttt tttctgcgct tcggctgcgg
     3901 ctttggcggc ttctgcttct gcttttttct ttgcgtctgc agccgctttc ttcgctgctt
     3961 cttccgcagc tttagcatct gcttcggcct tcgctttagc atctgccgcc gctttcgctg
     4021 ccgcctcttc agcttgcttc tgctttaact cggcctgttt tgcggcttct tcagcctgct
     4081 ttttctgctc ctgagccgct aaccgctctt tctcaagttg cttcaggcgt tcctgttcag
     4141 ccgcttgttt ctcacgcagt tcttcagcag cctgctgttc cttcatcttg cgctgttcat
     4201 cagaacgctt cgcgcttgat tcctggcttt gcatgcgttt gtactgctca actaccgcac
     4261 ctgaatcaac catgacagcg tcgatggacg aaccaccgcc gcctccggct gaagcttcta
     4321 tattctcatc gaacgaactc cagatcagcg ccgcaaataa gatgacatgc agcactgctg
     4381 aaataattat cgcccgcttg agcttgtcgt tttgttcggt tgcctttgac actctcggtt
     4441 tccaaaaact gttcgcctgt tacccgctct ctttcaagca agggaaacgc agatgtttag
     4501 ataggctgcg tcattaaacc aaccgatttc acacccgcac tatgtaacaa gttcagtgct
     4561 ttaattattt catcgtaagg cacatctttt gcgccaccga tcagaaagac cgttttcggg
     4621 ttggccttga aacggctgga cacttccgcc accacctgct ctggtggtaa acgctccagg
     4681 cgatctttct caaccaccac ggtgtactga ccaataccag acacttcaac aatcactggc
     4741 ggattatcgt tactgctcac cgcctgtgat tcaatagcgt ctggcagatc gacctccacg
     4801 ctctgggtga tgatgggcgc tgtcgccata aagatcagca acagcaccag cagtacgtcc
     4861 agcaacggta caatgttgat ttcggacttg agatcgcgac gacctcgtcc acgcgctctg
     4921 gccatggctt accccttgtt gctctcgcta acggtaaacg cctggcggtg cagaatcgcg
     4981 gtaaactctt ccataaagtt gtcgtaattc agttccagtt tgtttacgcg ctggttgagg
     5041 cggttataag ccattaccgc cggaattgcg gcgaacagac caattgcagt cgcaatcaac
     5101 gcttctgcga taccgggcgc aaccatttgc agtgttgctt gttttaccgc cccgagggcg
     5161 ataaaggcgt gcatgatccc ccagaccgta ccaaacagac caatatacgg gctgatggag
     5221 ccaaccgtac cgaggaacgg aatgtgcgtt tccagatttt caagttcacg gttcatggag
     5281 atacgcatag cacgcgacgc cccttccact acggcttccg gcgcatggct attggcacga
     5341 tgcaggcgca caaactcttt gaacccgctg taaaagattt gttccgaacc agtcagatta
     5401 tcccgtttcc cctggctctc ttgatagagg cgagagagtt cgattccaga ccagaattta
     5461 tcttcaaacg cttcggcttc gcgcgccgct gcgttaagaa tacgggtccg ctggataata
     5521 atggcccaag atgcgattga aaaaccaatc aaaatcaaca tgataagttt aaccagaagg
     5581 ctagccttca ggaacaaatc aaggatattc atgtcagtca ctgcttaaac tccgcgacaa
     5641 tagacttggg aagcgcacga ggcttcattt tgagtgggtc aacgcaaaca accagaacct
     5701 ctgcttcatt cagcaaagta ttctcggcgt tgacaatacg ttgcgtgaaa accaaagagg
     5761 tgccacgcat tgatgttatt tcagtctgta tttcgagcat atcgtcgagc cgcgcaggtg
     5821 cgtaatattc caccgtcatt ttacgtacca caaaggcaac gcgttcagcc atcagcgcct
     5881 gctgactgaa gtgatgatga cgcagcatct ctgtgcgtgc tctttcataa aaagcgacgt
     5941 aactggcgtg gtacaccaca ccaccggcat cggtatcttc atagtagacg cgaaccggcc
     6001 atcgaaacag cgttgtattc actttacatc ccggtaatgc aacaaaagtt agagctttta
     6061 aacgctgcta ctatacgcgc gcgattgagg tttgggaatg agggcaagtt aagggagcgt
     6121 aaaaatttat gggcctggag cggcccataa ttttttaaag agaattctga tttaaaagaa
     6181 gaagaaaatc agcccgacaa tgagaacaat atcggcaagc aatgggcaaa aaatcccttg
     6241 ccaaagaacc ttctgcggac gaaagcccac gccgtgaatc acaccagcac agacggccca
     6301 catcagcaat aaaccatgcc agatttccag ttcactggtc ttcgcggcaa aacgagatgg
     6361 gtcccaaaac atacatcctg ctaacagaag cgccatcacg aaggaaagcg cccgcagggg
     6421 gcgcttgtcc attaccgcat acaaagtcgc gataatctta ctcatcagat gtcttcttga
     6481 ccggctttgc ctgattcgac gtgttcaagc gccagcgcgg tgattacccc aaacgaacag
     6541 gcaagaagcg ttcccagaat ccatgcgaaa taccacattt ttagctcctt acttagtaca
     6601 gagagtgggt gttacgttca atatcttctt tggtgatacg accgaacatt ttccagtaac
     6661 accaggcggt gtagagcaga atgatcggta ccagaaccac cgcaacccag gtcatgacgt
     6721 taagcgtcag ctggctggaa gttgcatccc acattgtcag acttgcgttc atcatggtgc
     6781 tggacggcat cacaaacggg aacattgcga taccggctgt caggatgatg caggccagcg
     6841 tcagggagga gaacacaaac gcccacgcgg ctttatccat acgtgcagtc aggatggtca
     6901 gcagcggcag aaccacaccc agtgccggaa tagcccacag aattggcgtg ttgttgaagt
     6961 taaccagcca tgcgccagct tcacgaacca cttctttatt cagtgggtta gaggctgcgt
     7021 aatggtccat tgtcgatttc acgacataac catcgatacc gtacatcacc catacgccag
     7081 ccagtgcgaa acagaccagt gtcaccagcg cagccacctg agccgttgca cgggtacgca
     7141 ggtgcagttc gcccacggta cgcatttgca gataggttgc gccctgagta atgatcatcc
     7201 ctacgctcac cacgcctgcc agcaggccga acgggttaag caactggaag aagttaccgg
     7261 tgtagtacag acgcagatat tcatcaacgt tgaacggtac gccctgcaac aggttaccga
     7321 acgctacacc aattaccagc ggcggaacga agctaccaat gaagatgccc cagtcccaca
     7381 tgttacgcca gcgggtttct tcaatcttgg agcggtagtc aaaaccgacc ggacggaaga
     7441 acaaagacgc cagcacgagg atcatcgcca catagaagcc ggagaacgca gcggcataga
     7501 ccatcggcca ggcagcaaag agtgcgccgc ccgcggtgat cagccaaacc tggttaccgt
     7561 cccagtgtgg tgcaatggag ttaatcataa ttcgacgctc ggtgtcgtta cgaccgagga
     7621 aacgggtgag catgcccacc cccatgtcga aaccgtcagt gactgcaaaa ccaatcagca
     7681 gaacgccaac cagcagccac cagataaaac gcaatacttc ataatcgatc atttgacgac
     7741 tcctgtctta gcgtgccggc tgagtagtcg tggaagactg ctcaaagtga tagcgaccgg
     7801 ttttcaggct gcttgggccg aggcgtgcaa atttgaacat taagaacaat tctgccacca
     7861 ggaacagggt atacaggccg caaatcagca ccattgagaa gatgagatcg cctgcggtca
     7921 gtgacgagtt cgccacagct gtcggcagca cttcaccgat agcccacggt tggcggccat
     7981 attcagccac gaaccagccc gcttctacag caatccacgg cagcggaata ccgtacagcg
     8041 cggcgcgcag aagccatttt ttctcgccaa tgcggttgcg gatgacactc cagaaagaga
     8101 gcgcgatgat tgccagaagc aggaagccac acgccaccat gatacggaac gcaaagtaca
     8161 gcggcgctac acgcgggatg gagtctttgg ttgcctgttg aatctgcgct tcagtcgcat
     8221 cagccacgtt tggcgtatag cgtttcagca gcagaccgta accgaggtct ttcttcatgc
     8281 tattgaactg gtcacgaacc gcctggtcgg tagaaccaga acgcagttgt tcgagcagag
     8341 agtacgcctt catcccgtta cgaatgcgtt cttcatgctg caccatcagc tctttcaggc
     8401 cgataaccgg ggtatccacg gaacgcgttg caatgatgcc cagtgcgtaa gggatctgaa
     8461 tcgcaaattt gttcgtctcc tcttcctgat caggaatgcc gaacagagta aaggcagcag
     8521 gcgcaggttg cgtttcccac tcggcttcaa tagcagccag tttggttttc tgcacgtcgc
     8581 ccatttcgta gccggattca tcacccagaa caataacaga cagaacagca gccataccga
     8641 agctggcagc gatagcaaag gagcgtttag cgaaggcgaa gtcacgacct ttcagcatat
     8701 accatgcgct gataccgagg atgaacatcg cgccagtcac ataaccagac gctacagtgt
     8761 gaacgaattt cacctgagca accgggttaa gcaccagctc ggagaagctc accatctcca
     8821 tacgcatagt ttcaaagttg aaatcggacg cgattgggtt ttgcatccag ccgttcgcaa
     8881 ccagaatcca cagtgcggac aggtttgaac cgagcgccac cagccaggtg acacacatat
     8941 gctgaacttt acccagacga tcccaaccga agaagaacag acctacaaag gtggattcga
     9001 ggaagaaggc catcagacct tcgattgcca gcggcgcacc gaagatatcc cctacatagt
     9061 gggaatagta agaccagtta gtcccgaact ggaactccat ggtcagaccg gtagccacac
     9121 ccagagcgaa gttgatacca aacaacttgc cccagaactt ggtcatatct ttataaatct
     9181 gtttgccgga gaggacgtag accgtttcca taatggccag caggaacgcc ataccgagcg
     9241 tcagtggcac aaaaaggaag tggtacatcg cggtcaaggc aaactgtaag cgcgacagtt
     9301 cgactatatc taacatcatg actccttgct catcgcatga agactccgag agtgaacccc
     9361 gttagaaagg gtcacacgca tgccccaata gaaatttgtt tgaacacttt catcatacca
     9421 tgtgaatgca cacaaaaacg gcgtaaagaa aggttgcaaa catgttaata aaaactcaaa
     9481 ttgatcccac gtatatatta cgccgcaaaa tccttacaat aaacaggttt ttgttgaagc
     9541 aaatttgcat ttttcgacgg tgatcaattt atagctaaat taccgccttt cagccaattt
     9601 gatcgagaac aatttatctc tttttgatgc ccatttc
//
LOCUS       DAPGWD010000161         9631 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPGWD010000161 DAPGWD010000000
VERSION     DAPGWD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.162"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..738)
                     /gene="mak"
                     /locus_tag="RHX88_003727"
     CDS             complement(<1..738)
                     /gene="mak"
                     /locus_tag="RHX88_003727"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDV2232373.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPD"
     gene            863..1774
                     /gene="rdgC"
                     /locus_tag="RHX88_003728"
     CDS             863..1774
                     /gene="rdgC"
                     /locus_tag="RHX88_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDV2232374.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(1852..1935)
                     /locus_tag="RHX88_003729"
     CDS             complement(1852..1935)
                     /locus_tag="RHX88_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232375.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(1932..2273)
                     /locus_tag="RHX88_003730"
                     /pseudo
     CDS             complement(1932..2273)
                     /locus_tag="RHX88_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(2421..2705)
                     /gene="ppnP"
                     /locus_tag="RHX88_003731"
     CDS             complement(2421..2705)
                     /gene="ppnP"
                     /locus_tag="RHX88_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDV2232376.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(2777..3454)
                     /locus_tag="RHX88_003732"
     CDS             complement(2777..3454)
                     /locus_tag="RHX88_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDV2232377.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(3712..3903)
                     /gene="yaiA"
                     /locus_tag="RHX88_003733"
     CDS             complement(3712..3903)
                     /gene="yaiA"
                     /locus_tag="RHX88_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDV2232378.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(3953..4477)
                     /gene="aroL"
                     /locus_tag="RHX88_003734"
     CDS             complement(3953..4477)
                     /gene="aroL"
                     /locus_tag="RHX88_003734"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDV2232379.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(4660..5118)
                     /locus_tag="RHX88_003735"
     CDS             complement(4660..5118)
                     /locus_tag="RHX88_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDV2232380.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5238..6047
                     /gene="proC"
                     /locus_tag="RHX88_003736"
     CDS             5238..6047
                     /gene="proC"
                     /locus_tag="RHX88_003736"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDV2232381.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEADVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(6064..7179)
                     /gene="adrA"
                     /locus_tag="RHX88_003737"
                     /gene_synonym="dgcC"
     CDS             complement(6064..7179)
                     /gene="adrA"
                     /locus_tag="RHX88_003737"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDV2232382.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDYFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(7281..7601)
                     /gene="psiF"
                     /locus_tag="RHX88_003738"
     CDS             complement(7281..7601)
                     /gene="psiF"
                     /locus_tag="RHX88_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDV2232383.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(7720..9135)
                     /gene="phoA"
                     /locus_tag="RHX88_003739"
     CDS             complement(7720..9135)
                     /gene="phoA"
                     /locus_tag="RHX88_003739"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDV2232384.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(9236..9496)
                     /gene="iraP"
                     /locus_tag="RHX88_003740"
     CDS             complement(9236..9496)
                     /gene="iraP"
                     /locus_tag="RHX88_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDV2232385.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         2361 a         2536 c         2308 g         2426 t
ORIGIN      
        1 atccggatcg agaatattca cgacatgtgc cagcgatttt gccagccgca gctcgtagcg
       61 acgcaatgcc agttccgcta ccggatcgct ttcttcaacc aggctgataa tttcactgcc
      121 tttcagcgca tgtccgctca aacgacgata atctgtggca aatcccgtgc ccgaaataaa
      181 ggtttcaata caaccttgtt taccgcaata acaagggact tcctcgcgat aacgcagttc
      241 gtcttcgtcc atccacggta gcggattgtg tccccactca cctgccgtgc cattgccgcc
      301 gatatgcgcc cgcccattga atgccacgcc cgcgccgcat cccgtgccga taatcacggc
      361 aaatactgtc tgcgctcccg ctgccgcgcc atctactgct tctgaaaccg ccagacagtt
      421 ggcgtcattt gccagccgca cttcccgctg caacctcgcg cttaagtctt tatcgaatgg
      481 ctgaccgttg agccaggttg aattggcatt cttaaccaca ccggtgtaag gcgaaattga
      541 gccaggaatg cccataccta ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac
      601 caacgtggcg atcgtttcaa tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg
      661 gcggtacaac tgctcccctg catcgcccag tgcaatcact tcagttttgg tgccgcctaa
      721 atcgatacct atacgcacgg tactctcctt atttttttca atatcaatag cgtagagacg
      781 gacaaccgga ttggcaatgc aaggccgccg acaattcgtt atcatgcccg ctaaatttaa
      841 cgacaaggcc gtggaaatta tcatgctgtg gttcaaaaat ttaatggttt accgtcttag
      901 ccgcgagatt tcgctgcgtg cagaagagat ggaaaaacag ctagcctcga tggcatttac
      961 cccatgcggc agccaggaca tggcgaagat gggctgggtt cctccgatgg gatcgcacag
     1021 cgatgcgtta acgcacgttg ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa
     1081 aatcctcccg tctccggtga ttaaacaggc gctggaagcg aaaatcgcca aactggaagc
     1141 ggaacaggcg cgtaagctga agaaaaccga aaaagattcg ctgaaagacg aagtgctgca
     1201 ctctctgctg ccgcgtgctt tcagccgttt tagccagaca atgatgtgga tcgacacggt
     1261 taacggtttg attatggtgg actgcgccag tgccaaaaaa gcggaagata cgctggcatt
     1321 actgcgtaaa agcctggggt cgttaccggt tgtaccattg agcatggaaa acccgattga
     1381 actgacgctg accgaatggg ttcgctccgg tagtgcggca cagggcttcc agctgcttga
     1441 tgaagccgag ctgaaatcgt tgctggaaga tggcggcgtg atccgcgcga agaaacaaga
     1501 tctgaccagc gaagagatca ccaatcacat tgaagccgga aaagtggtga ctaaactggc
     1561 gctcgactgg cagcagcgca ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct
     1621 gaagttctgc gacgagctgc gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca
     1681 gcgttttgat gccgatttca tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct
     1741 gattgaagga ttaggtggcg aagcacaacg ttaattgctg attttccttt aatgccggat
     1801 gcgacgcctg ccgcgcctta tccggcgtac gaagccacac caggcatata attattcgct
     1861 acggcgggca ataattttta gcgcagcaat attatgcgtt ttatgctgta acttgctcca
     1921 tggacgttgt gtcattgttt ttcctcaagc cagtgacgcg cttcactgcg atttttccgc
     1981 attgccccgg ttttcactaa atcccgtaac agcgaaagat cgggttgttc gacaaataag
     2041 agtaatgatg gatcctcttt taaccacgtc gttttcacat cggcagcgag ctgtggatta
     2101 ttcatagcta gcgaccgatg ccgagactcg gtcaatgtcg ttaacaacga cgccggggta
     2161 tgtgcgttgc gtaatgccgt ttggcgcaaa tgatcattgc gactttgcag caatgcacgc
     2221 caccattcat catctgattt atcttttatc gtagccagtt ccgcaacaat atctggagat
     2281 cttcactgat atttatatca accttgatga attatgaata taccggaaat tgaaaaaatc
     2341 gcagcaaagg aaatattatt atgggttaaa gagaggatat ttcagaaaaa tgccctcccg
     2401 ttcaagggga aggcgaggaa ttacagatag cggcacagat aagaggtggg ttcggcaact
     2461 tgcagatgaa actcactgtg accgggaaca ttaaacaccg aaccggcttc atacacctgc
     2521 cagtcggtcg cgtcaggcag taacacattc agcgcgccac tgattaccgt catctcttcc
     2581 ggctcagcgg tgctgaaggt gtattcgcct tcaaccataa cacccacgct ggcgcgacca
     2641 gtgctgctgc tggaaaagcc gattgatttc actttgccgg aaaagtactc attactttga
     2701 agcataaact ggccctgttt ggtagttgaa catctacttc actatagggg ccagaggcgc
     2761 tgactgtcac gcaaaattac accagtaatt ccgcagccag ccgtgcaatc aatacgttag
     2821 acagcaagac aggaacatcg agctgttttt gcagtaaatc gcgatgacgt tggtgaaatc
     2881 ctaaacaatc cagcatgatg acatcagccc cttttgccag taattctttc ccggcatcaa
     2941 tgattttttg ttctgaatca tgaatggggt tacccaatga aaataccggc gatttctgca
     3001 aaatttgcca tttttgcgcc tgaacgggca gcatctcctc aaccggaacg ataaccccca
     3061 cctgatgatc ttcaacaata gaggaaacca gtggaggcaa tattcgcgac ggctcaagaa
     3121 agatcgtatt acgcgcagtc atactactaa tgtttgctgt actcattaat aaaatgacgt
     3181 cataaccctg attatcgagc acttcaacca caccttgcag gtctcgctcc actttacgac
     3241 gcgaaacatg ggccagctgg ttgtcattta ataatgtgag aatggtgtct tcgcctgctt
     3301 ctggcgcgta ctccgccatc acttcttcac gacttaactt ccccagcagg ctatgatggg
     3361 aaatattatc ttcgtcaatg tattccgtca ggagcggcaa aacctcctgc ataggtacaa
     3421 tgccgatggt caggatcgcc aacgacgcac tcattgttca tccaccttac ttttctttcg
     3481 tcgttaatta ccgggcaagt gtgaagcacc actgctgaca cttacttgtg ctacaaatga
     3541 caaaaagcgt agcagcagac gtgcacggca taatgtaaag attccaaatg attccagtaa
     3601 tggattgtta ttgtttaata ttctaattat tagaaaaaca tgaattatga aaaatgtgac
     3661 gcagatccct tgtagtggca ctcagaatcc cttccgagca gtctgatgcg gtcactcttt
     3721 gtcagggtcc tcatagcgtt ttttcgcatc catcgcttcc tgagccgtcg gatgttcact
     3781 gatcaacgag tctggtttag gatgatcggc tctgagttgg taccaggtta ccgtctgtcc
     3841 tggctttcct ttttcaatcg tcactatata tgcttcacga ggataaggcg gtttcgttgg
     3901 catcgttctt ccttatttca cgggatgaac gttaagtata ggcgctctaa aatcaacaat
     3961 tgatcgtctg tgccagggcg ctacgaattt cagaaatcac ctggctgggt tcgtttgttg
     4021 cgtcgatgat aatatgcgca acttcgcgat atagcgcatc gcgttcttcc agcacttcct
     4081 gaacttcttc gctcagcggt tttcccgtta aggttggccg taaatcttct tccggtgcag
     4141 cttgcagtcg gttaaccagg actgatactg gcgcacacaa ataaaccacg atcccgttat
     4201 tttgcataaa gtgacgatta aattccgtca gaataatgcc gccgcctgta gcgataacgg
     4261 tggatggcgc agttaccgct tccagcgccg ccgtttctct ggcgcgaaat cccgcccact
     4321 cttccctttc gacgatctcc gcgaccgtca tattgagctg tgattgcaac cactgatcgg
     4381 tatcgacaaa ccgacggtta agcgaatcgg caagggccat tccgaccgtt gttttaccac
     4441 agccccgagg cccgatcaga aaaagaggtt gtgtcatcgt gggttttccc caataggtcg
     4501 caatgcggcg aaagccggtg tcatgagaat agcgatcata ccatcaaact agtacaattt
     4561 cgattgtaaa gaaaaaattc cacttaaagt gaaaatctca atacatccct tactatacca
     4621 ataaatattc aagaatgaag tgtaaataat aaattacatt tagccacgac tacgttgcac
     4681 ttccagccac cacttctcca gctccgcggc aaaggcctgg cggtcacgtt gtgaaaggct
     4741 atctggtccg ccggtctgga tcccactggc acgtaaggta tccataaaat cgcgcatcgt
     4801 caggcgctca cgaatggtcg ctggcgtgta acgttcgccg cgcggattaa gcgccgctgc
     4861 gcctttctcg atggcttcag cagccaaagg tatatctgcg gtgatcacca aatcgcccgc
     4921 ttcacactgc cggacaattt cgttatcggc aacgtcgaaa cctgccgcga cgcgcagcgt
     4981 acgaataaat cgcgatggcg gcacgcgtaa actctggttt gctaccagta ccagcggcat
     5041 ctgcatacgt tccgccgcgc gatacaaaat ctctttaatt acattgggac acgcgtcggc
     5101 atccacccaa attgtcataa agtcatcctt tgttgggtaa tcctctattg tgtcgcgctt
     5161 ttgccttccg gcatagttct gtttatgctt ctgccagcga ttatcaaaac aatgaatttc
     5221 acggcaggag tgaggcaatg gaaaagaaaa tcggttttat tggctgcggc aatatgggaa
     5281 aagccattct cggcggtctg attgccagcg gtcaggtgct tccagggcaa atctgggtat
     5341 acaccccctc cccggataaa gtcgccgccc tgcatgacca gttcggcatc aacgccgcag
     5401 aatcggcgca agaagtggcg caaatcgccg acatcatttt tgctgccgtt aaacctggca
     5461 tcatgattaa agtgcttagc gaaatcacct ccagcctgaa taaagactct ctggtcgttt
     5521 ctattgctgc aggtgtcacg ctcgaccagc ttgcccgcgc gctgggccat gaccggaaaa
     5581 ttatccgcgc catgccgaac actcccgcgc tggttaatgc cgggatgacc tccgtaacgc
     5641 caaacgcgct ggtaacccca gaagataccg ctgatgtgct gaatattttc cgctgctttg
     5701 gcgaagcgga tgtaattgct gagccgatga tccacccggt ggtcggtgtg agcggttctt
     5761 cgccagccta cgtatttatg tttatcgaag cgatggccga cgccgccgtg ctgggcggga
     5821 tgccacgcgc ccaggcgtat aaatttgccg ctcaggcggt aatgggttcc gcaaaaatgg
     5881 tgctggaaac gggagaacat ccgggggcac tgaaagatat ggtctgctca ccgggaggca
     5941 ccaccattga agcggtacgc gtactggaag agaaaggctt ccgtgccgca gtgatcgaag
     6001 cgatgacgaa gtgtatggaa aaatcagaaa aactcagcaa atcctgatga ctttcgccgg
     6061 acgtcaggcc gccacttcgg tgcggttacg tccggctttc tttgctttgt aaagcgccaa
     6121 atctgccgat ttcaaccact cacgatagtg actcatttgt gggttcagcg gcgcaacccc
     6181 cacactaatc cgtaaagtta cctgtggcgt attcggcaag cgtaacgtgt ttagcccttc
     6241 acgcacccgt aacatggcgg taatggcgct ctcagctggc gtaccggaca tgattactgc
     6301 aaactcatcg ccgccaaacc gaccaatcac atcgctaccg cgcagggtaa tttgtaactg
     6361 tcgggtaagc gccacaatcg cttcatcgcc cacatcatga ccccaggtat cgttgatgct
     6421 cttgaaatag tcgatatcga taatcagtaa cgttgcatcg cgattatgcc gccgacagtt
     6481 atcaaattca ttgcgtaaca tagtttccca atgacgtcgg ttatacacgc ccgtcatgcc
     6541 gtcgcgggta ctcatgacct gcaacctgcg tttatgttcc gccagtttgg ttgccgtctg
     6601 gtagctgacc cagccaaaca gcagaggata aacgacaata atgggaaggg agagccacca
     6661 ttccagcggc gcactattga acgacacggt aatgcccgta agctcgaggg tgacaaggca
     6721 ggaaaccacc atcaacacca gacccgcgac aaacagacgg gggccgcctg cccccatcaa
     6781 attcagacac ataatcatca acatagcggt ggaaggcagc acgtttacgc ccattacgcc
     6841 tacccacatt cccgctaata ctgcatcggt ttttaagttg taaatttccc ggctaagcgg
     6901 atcgacggcc ctgctcgcta tctgccaggc taaatgcggc cagacgaacg cccagccgac
     6961 caacaccagc caccaccagc ccggcggcgg gtgcgaaacc agcgttgaag caatcggtaa
     7021 gaacatgcca gccaggccaa ccgcacgggg tagtctgacg cgacgggcga agcgcagccc
     7081 ggaccgctga tgttcgtttt gaggagttaa aggaggttcc tccccatgcg ccgccgcttt
     7141 tttgaaaaag ttttcatcat tcattatttt tgggaacatt cagaaacaat tttcccaaat
     7201 tatagagacg gggctaaagg cagaagtatg aaatttcggg tgagccactt tcgtagctca
     7261 cccgttagca ctatcaggta tcaggcggct ttcttgaggc aggcactcat aaacttatta
     7321 cgatcatcac ctttcagaga ttgttgcgtt gcttgattat tacattcgcg catcttttgc
     7381 tgctgtggcg tcaaactttt ttcaccaggc gcagacttgc tgttcttcag gcaatcactc
     7441 atgtaggtct tacgagcatc ccctttcaac gcctgcgccg tcgcctgctg attacaggag
     7501 gtcatacgct gttgttgtgg ggttaacgtt ctctcggcag cgccgacggt ggttaaaaaa
     7561 accagaccaa aaagcaaggt aaccagtaat gttattttca tagcaccatc cctcttcatg
     7621 ttttaaccat gagcgtttgc gcccgtgatc tgccattaag tctggttgct aacagcaaaa
     7681 aaaccacccg gcagcgaaaa ttcactgccg ggcgcggttt tatttcagcc ccagagcggc
     7741 tttcatggtg tagaagagat cggtctggtc ggtcagtcca acaacattgg cggcatgcgg
     7801 gccatacgcc gcaatacgca actgactgcc ggtatgttct tgtgaatcct cttcggaatt
     7861 cccgtaactc atcaccatca ctgcgccatc tttagtattt agcgcctgag tgaggcccgg
     7921 cgctttggta tccggcgcaa caacctggct ggcgtgggcg tgatcagcgg tgactatgac
     7981 cagcgtattg ccatcctttt tagcgaattc cagcgcccgt tgtacggctt catcgagatc
     8041 gaccgtctcg ccaatttgcc cacaaggatt cgcagcgtga tcctgtttat cgattgacgc
     8101 accttcaact tgcaggaaaa atcctttctc atttttactc aacaattcaa tggctttgtc
     8161 ggtcatctgc gccagggttg gtacactgtc attacgttgc ggattaggcg tacaggtgac
     8221 tgcgggctta tcgatattgc cgtggtacgt tgctttcggt cctagccagc gcactggcat
     8281 attgccgtca gcaaacagtc caagcagagg tttttgctga ttcgcttccg tcaccgaatt
     8341 cagtgaggca gcatcgctga ccaactgata accacgcgcc tgtgcctgtt cacgcagcgt
     8401 ttttccctgc cattcaccgg cggttgccgt ttcagcaaag gtttttgcgc caccgccaag
     8461 cgtaacgtcg gcacgagcgt taagcagctg ttcggtaatc gatccttttc cgcctttttc
     8521 cagagcgtta cccggacatt tttcactggt cgcgctcgga ccgtagcatt tgcgcgaagt
     8581 cacatgtgcc accagcgcag cgggcgtggc atcctgcaac tctgcggtag aaacgttacc
     8641 ggtcgccaga cctgcggctt ttgccatttc cagaatcgtt gggtgatctt tttcgtgaat
     8701 atcgacgccc agcgcgccgt tataggtttt gacaccggtt gaccaggcgg ttgctgatgc
     8761 agccgagtcg gtgacgtagt ccggtttgcc ggttttttta ttcagcgcat agtgagtgta
     8821 ttgcccggta agcggtaagg catcgatacc tttaaaaaag ccgcccgcac cttcggcata
     8881 attacgtgcg gcagtaattt ccgaatcccc catcccatcg ccaatcagca aaataatatt
     8941 ttttgcaggt ttatcgctaa gagaatcacg cagagcggcg gtctgatcac ccgttaagcg
     9001 gcgagcaccg ccgggtgcag taatatcgcc ctgagcagcc cggttttcca gaacaggcat
     9061 ttctggtgtc cgggcttttg tcacaggggt aaacagtaac ggtaagagtg ccagtgcaat
     9121 agtgctttgt ttcactttat tttctccatg tacaaataca ttaaaaaata aaaacaaagc
     9181 gactataagt ctcggccgtg acaactttat gacagctgtt gaaaagatta actttttact
     9241 gacgaggatg cttcaataac ttctttacgt aatcgcgcag cagctccgta tcgtcgtcag
     9301 gaatgctggc atcgggcttt acctcgtaca gcgccccctc tacctgatca atcaaccgct
     9361 gttggtcatt ttgcgccata ttgcgaagca ttgcagtgac gataatctcc aaagcttcta
     9421 cctgcgcaca cagttctttc gactcttctt ctttttgggc aagcttaaat aacaactcag
     9481 caatgagatt tttcatgtct gtatttcctt atccaaagta tggagaagtt aacattatgt
     9541 ttcacaactt catagtgtct ggaagtatta tttttatgac aaaggaaata ctttgcgcaa
     9601 atattttttg tttgattacc agcgaaacgt t
//
LOCUS       DAPGWD010000162         9553 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPGWD010000162 DAPGWD010000000
VERSION     DAPGWD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.163"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            93..2354
                     /locus_tag="RHX88_003741"
     CDS             93..2354
                     /locus_tag="RHX88_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDV2232386.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(2588..3871)
                     /locus_tag="RHX88_003742"
     CDS             complement(2588..3871)
                     /locus_tag="RHX88_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDV2232387.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4023..4499)
                     /locus_tag="RHX88_003743"
     CDS             complement(4023..4499)
                     /locus_tag="RHX88_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDV2232388.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDEVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(4558..5847)
                     /gene="bioA"
                     /locus_tag="RHX88_003744"
     CDS             complement(4558..5847)
                     /gene="bioA"
                     /locus_tag="RHX88_003744"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDV2232389.1"
                     /translation="MTTDDIAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            5934..6974
                     /gene="bioB"
                     /locus_tag="RHX88_003745"
     CDS             5934..6974
                     /gene="bioB"
                     /locus_tag="RHX88_003745"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDV2232390.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            6971..8125
                     /gene="bioF"
                     /locus_tag="RHX88_003746"
     CDS             6971..8125
                     /gene="bioF"
                     /locus_tag="RHX88_003746"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDV2232391.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSEEGDARREKLAALITRFRAGVQGSPFTLADSRSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEMLHGNG"
     gene            8112..8867
                     /gene="bioC"
                     /locus_tag="RHX88_003747"
     CDS             8112..8867
                     /gene="bioC"
                     /locus_tag="RHX88_003747"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDV2232392.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPGAIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            8860..9537
                     /gene="bioD"
                     /locus_tag="RHX88_003748"
     CDS             8860..9537
                     /gene="bioD"
                     /locus_tag="RHX88_003748"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV2232393.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LLALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         2267 a         2497 c         2639 g         2150 t
ORIGIN      
        1 cttgtcgcct tcctgcaact caaattattt agagtatatc catttattat ctttctgcgc
       61 acttcacggt gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt
      121 ttctcgccag taataacgaa ataattgctg aagaacattt caccggcgaa attaaaaaag
      181 aagaagccaa aaaaggcact attgcctggt ctattctctc ttcgcataat acgtccggaa
      241 atatggataa acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg
      301 gtattgtaca gaccgctaaa gcgtccggta tggaacgttt cccgctgccg tatgtgctga
      361 ccaactgcca taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt
      421 ttggtttatc ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca
      481 tccatcaata tatgcgtgag atgatggcag gcggcggcaa aatgatcctc gggtcagaca
      541 gccacacccg ttacggtgca ttagggacaa tggcagtcgg tgagggcggt ggtgagttgg
      601 taaaacagct gcttaatgac acctgggata tcgactatcc gggagttgtt gcggtgcatc
      661 tgaccggaaa accagcgccg tatgtggggc cgcaggatgt ggcgctggct atcattggcg
      721 cggtgttcaa aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta
      781 gcgcgctctc taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt
      841 taagctccgt ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg
      901 gccaggatta ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg
      961 ttgatttaag cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg
     1021 aaatcgacac actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg
     1081 aacgcgtggc gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc
     1141 tgaaagtgca gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg
     1201 cggcggcgaa tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcagttt
     1261 acccgtcatc acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga
     1321 ttggcgcagg cgcaatcatc agaaccgcgt tctgcggccc atgctttggc gcgggcgata
     1381 cgccaatcaa caacggatta agtattcgcc acaccacgcg taacttcccg aaccgcgaag
     1441 gctctaagcc agctaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg
     1501 ctgcgactgc ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca
     1561 acgtgccgga gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg
     1621 tgaaaggggc aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat
     1681 tgggtgcgct gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtga
     1741 ccaccaccga cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg
     1801 gtctggcgga gtttaccctg tcacgccgcg atcccggtta tgttggcaga agtaaagcga
     1861 ctgctgagct ggaaaatcag cgtctggcgg ggaatgtcag cgagctgaca gaggtgtttg
     1921 cgcgcattaa gcagattgct ggtcaggagc atattgatcc gctgcaaact gaaattggca
     1981 gcatggtata tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg gcgagctgcc
     2041 agcgtgtgat tggcggtctg gcgaatattg ccgaagagta cgcgactaaa cgctaccgtt
     2101 ctaacgtcat caactggggg atgttaccgc tgcagatggc ggaagtgcca acctttgaag
     2161 tgggggatta catttacatc cctggcatta aagcggcgct ggataatccg ggtacgacgt
     2221 ttaaaggtta tgtgatccat gaagatgcgc cggtaacgga aattacgctc tatatggaaa
     2281 gtctgactgc tgaagagcgc gagattatca aggcgggtag tttgattaac ttcaataaaa
     2341 accgtcagat gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt attgtcggat
     2401 gcgatgcttg cgcatcttat ccgacctacg aatcgcatcg aatctgtagg ccagataagg
     2461 cattttcgca gcatccggca cttattgtcg gatgcgatgc ttgcgcatct tatccgacct
     2521 acaaatcgca tcgaaccgta ggccggataa ggcgtttacg ccgcatccgg caaatagtta
     2581 atagctctta cttcttcgcc tctgcaacca ctttactacc cacgccgcgg ttattgtatt
     2641 cccacatgcg gttgtagtta gtgtcattca gattgcgctg tatttcgtcg ttatcatcta
     2701 cgctgccggt attacccgca aacggacgat tagagatcac cgcatcggcc cacggtttag
     2761 ccgtgttaaa accttcgttg atggcgctat cacggatcac cacctgaccg ttggtattgg
     2821 catcaacatc cagcgagcgg cccagttgcg ccacaccatc accgaaagca ttgaaacggc
     2881 tgtttacggc gaggaaaccg tagtaaatgt tggacagcgt agccggtgca aacacatacg
     2941 cttcttgctg agtacgtgag ttcaccacgc ggaattcggt gttatcgaac accactgcgc
     3001 cgcgaccaga aacgatatcc acatcccctt caatgtagct gttggtcacc agcgtacgcg
     3061 gctgacggtt ggtttccaga cggttctgca caccgctgtt ggtgacaaag aaggtgttct
     3121 gacgaccgag aatgttaacg ttgttaatct gtacctggtc accatcagta cgcagtgcca
     3181 ccgccggatg gttacctgca tctacgctat cgcccagcgt gttttcgatg gtcagatttt
     3241 gcagttgcag gccattgttt tgtgaccaga agaccgcaga gcagagaaca ccgatactgt
     3301 cgctgcgttt gctctggcag ctatcgtaca tataccacgc tggtttacct ggcatatatt
     3361 tgccgcgcgg gttgacgtcg tgacgccagt cggcagggct catgccccca tcaagggaaa
     3421 gcccaatctt cacatcaatc ggtttttcac ctgtaccgta cagagtaatt ccacccggag
     3481 cagcagggac atacaccgtt ccctgatact caccaggcat cacggcaata tactggcgct
     3541 tgttagtgcg cttgataatt gccgcatcta ccgccgcctg aatcgtggta tgcgttacac
     3601 cttgagtacc cgccgggccg acaacaaagt caggttgcgc aggcagggta atcggggaag
     3661 gattccacgc tgcagcacct ggtgtcaggg atgcaaaata gtgttgagca tcgaaattct
     3721 gcgcttcttt tgccgacaga atcgggcgag aagaggtacc aggcgcggtt tgatcagaag
     3781 gacgttgatc gggtggtgtt gagctacagg cggtcagcgt cacgccaaaa gccaatgcca
     3841 gcgccagacg ggaaactgaa aatgtgttca caggttgctc cgggctatga aatagaaaaa
     3901 tgaatccgtt gaagcctgct tttttatact aacttgagcg aaacgggaag gtaaaaagac
     3961 aaaaagttgt ttttaatacc tttaagtgat accagatggc attgcgccat ctggcagagt
     4021 gattaactaa acatcgcagt aatcgaggcg cttgccagag agtggaaatg aacgttaaac
     4081 ccgaccatcg cgccgctggc accttcatcg acatcaatac gttctacatc cagcgcgtga
     4141 acggtaaaaa tgtagcgatg agtttcgcct ttcggcggtg ctgcgccatc gtatccggtt
     4201 ttaccaaaat cggtacgcgt ctgcaaaacg ccgtctggca ttgctaccag accagagcca
     4261 aacccttgcg gtaatacgcg tgtatcagcg ggtaagttaa caactaccca gtgccaccag
     4321 ccggagccgg ttggcgcatc cgggtcgtag caggtgacaa caaaactttt cgttcccgca
     4381 ggaacttcat cccacgccag atgcggtgaa atattatcgc catcgtaacc catgccgtta
     4441 aagacatgac gatgcggcaa cttatcgcca tcgcgcagat cgttactgat gagtttcatg
     4501 aaccctcctt tcttgtttgc agaaagtgta gccagaaacc ctcacgctga cttcccgtta
     4561 ttggcaaaaa aatgtttcat cctgtaccgc gcggttaacc gctgcggtca gacgctgcaa
     4621 ctgttgcggg agaataatat agggcggcat caggtaaatc agtttgccaa aaggccggat
     4681 ccagacaccc tgttcgacaa agaatttttg cagcgccgcc atattcaccg gacgagtggt
     4741 ttcgaccaca ccgattgccc ccagtacgcg cacatcggca accatttcgg catcacgggc
     4801 gggggcaagt tgctcgcgca gctgtacttc aatagccgcc acctgctgct gccattcgcc
     4861 ggattcgata atcgccaggc tggcgtttgc tgccgcgcag gccagcggat tgcccataaa
     4921 agttggcccg tgcataaagc aaccggcttc gccgttactg atggtttccg caacctcgcg
     4981 cgtggtgagc gtggcggaaa gggtcattgt gccgccggtt aaggctttac cgaggcacaa
     5041 aatgtccggc gcgatttctg catattcaca ggcaaacagt ttgccggtac gaccaaatcc
     5101 ggtggcgatc tcgtcggcaa tcagcaagat accttcgcga tcgcacattt tgcggattcg
     5161 ttttaaccat tccggatggt acatgcgcat cccgcctgcg ccctggacaa tcggctcaat
     5221 gatcaccgct gcgatttcat gacgatgcgc cgccatcagg cgggcaaaac caaccatatc
     5281 gcgctcatcc cattcaccat ccatgcggct ttgcggggcg ggagcaaaca agttttctgg
     5341 cagatagcct ttccacaggc tgtgcattga gttgtccgga tcgcacaccg acatcgcgcc
     5401 aaaggtatcg ccatgataac cattgcggaa ggtcagaaaa cgctggcgcg cttcgccttt
     5461 ggcttgccag tactgcaacg ccattttcat cgccacttcc accgctacgg aaccggagtc
     5521 cgcgagaaaa acgcactcca gtggttgcgg cgtcatcgcc accagtttgc ggcacagctc
     5581 aatggctgga gcatgagtga taccgccaaa catcacatgc gacatggcat caatttgcgt
     5641 cttcatcgcc gcattaagct gcgggtgatt gtagccgtgg atcgccgccc accaggacga
     5701 cataccgtca accaggcgtc tgccgtcaga caaaattagc tcgcaacctt cggcgctcgc
     5761 caccggataa accggcagag gggaggtcat ggatgtgtat gggtgccaga tatggcgttg
     5821 gtcaaaggca atatcgtccg ttgtcataat cgacttgtaa accaaattga aaagatttag
     5881 gtttacgagt ctacaccgaa ttaacaacaa aaaacacgtt ttggagaagc cccatggctc
     5941 accgcccacg ctggacattg tcgcaagtca cagaattatt tgaaaaaccg ttgctggatc
     6001 tgctgtttga agcgcagcag gtgcatcgac agcatttcga tcctcgtcag gtgcaggtca
     6061 gcactttgct gtcgattaag accggagctt gtccggaaga ttgcaaatac tgcccgcaaa
     6121 gctcgcgcta caaaaccggg ctggaagccg agcggttgat ggaagttgaa caggtgctgg
     6181 agtcggcgcg caaagcgaaa gctgcgggat cgacgcgttt ctgtatgggc gcggcgtgga
     6241 agaatcccca cgaacgcgat atgccgtacc tggaacaaat ggtgcagggg gtaaaagcga
     6301 tggggctgga ggcgtgtatg acgctgggca cgttgagtga atctcaggcg cagcgccttg
     6361 cgaatgccgg gctggattac tacaaccaca acctggacac ctcgccggag ttttacggca
     6421 atatcatcac cacccgcact tatcaggaac gcctcgatac gctggaaaaa gtgcgcgatg
     6481 ccgggatcaa agtctgttct ggcggcattg tgggcttagg cgaaacggta aaagatcgcg
     6541 ccggattatt gctgcaactg gcaaacctgc cgacgccgcc ggaaagcgta ccaatcaaca
     6601 tgctggtgaa ggtgaaaggc acgccgctgg cggataacga tgatgtcgat gcctttgatt
     6661 ttattcgcac cattgcggtc gcgcggatca tgatgccaac ctcttacgtg cgcctttctg
     6721 ccggacgcga gcagatgaac gaacagactc aggcgatgtg ctttatggca ggcgcaaact
     6781 cgattttcta cggttgcaaa ctgctgacca cgccgaatcc ggaagaagat aaagacctgc
     6841 aactgttccg caaactgggg ctaaatccgc agcaaactgc cgtgctggca ggggataacg
     6901 aacaacagca acgtcttgaa caggcgctga tgaccccgga caccgacgaa tattacaacg
     6961 cggcagcatt atgagctggc aggagaaaat caacgcggcg ctcgatgcgc ggcgtgctgc
     7021 cgatgccctg cgtcgccgtt atccagtggc gcaaggagcc ggacgctggc tggtggcgga
     7081 tgatcgccag tatctgaact tttccagtaa cgattattta ggtttaagcc atcatccgca
     7141 aattatccgt gcctggcagc agggtgcgga gcaatttggc gtcggtagcg gcggttccgg
     7201 tcacgtcagc ggttatagcg ttgcgcatca ggcgctggaa gaagaactgg ccgagtggct
     7261 gggctattcg cgggcactgc tatttatctc tggttttgcc gctaatcagg cagttattgc
     7321 cgcgatgatg gcgaaagagg accgtattgc tgccgaccgg cttagccatg cctcattgct
     7381 ggaagctgcc agtttaagcc cgtcgcagct tcgccgtttt gctcataacg atgtcactca
     7441 tttggcgcga ttgcttgctt ccccctgtcc ggggcagcaa atggtggtga cagaaggcgt
     7501 gttcagcatg gacggcgata gtgcgccact ggcggaaatc cagcaggtaa cgcaacagca
     7561 caatggctgg ttgatggtcg atgatgccca cggcacgggc gttatcgggg agcaggggcg
     7621 cggcagctgc tggctgcaaa aggtaaaacc agaattgctg gtagtgactt ttggcaaagg
     7681 atttggcgtc agcggggcag cggtgctttg ctccagtacg gtggcggatt atctgctgca
     7741 attcgcccgc caccttatct acagcaccag tatgccgccc gctcaggcgc aggcattacg
     7801 tgcgtcgctg gcggtcattc gcagtgaaga gggggacgct cggcgcgaaa aactggcggc
     7861 actcattacg cgttttcgtg ccggagtgca gggttcgccg tttacgcttg cggattcacg
     7921 tagcgccatc cagccattga tcgtcggtga taacagccgt gcgttacagc tggcagaaaa
     7981 actgcgccag caaggttgct gggtgacggc gattcgcccg ccaaccgtac ccgctggcac
     8041 cgcgcggcta cgcctgacct taaccgccgc ccatgaaatg caggatattg accgtttgct
     8101 ggagatgctg catggcaacg gttaataaac aagccattgc agcggcattt ggtcgggcag
     8161 ccgcgcacta tgagcaacac gcagatctac agcgccagag tgctgacgtc ttactggcaa
     8221 tgctcccaca gcgtaaatac acccacgtac tggacgcggg gtgtgggcca ggctggatga
     8281 gccgccactg gcgagagcgt cacgcgcagg tgacggcgtt agatctctcg ccgccaatgc
     8341 ttgttcaggc acgccagaag gatgccgcag accattatct ggcgggagat atcgaatccc
     8401 tgccgttagc gactgcgacg ttcgatcttg catggagcaa tctcgcggtg cagtggtgcg
     8461 gtaatttatc cacggcactc cgcgagctgt atcgggtggt gcgccccgga ggcgtggtcg
     8521 cgtttaccac gctggtgcag ggatcgttac ccgaactgca tcaggcgtgg caggcagtgg
     8581 acgagcgtcc gcatgctaat cgctttttac caccaggtgc aatcgaacag tcactgaacg
     8641 gcgtgcatta tcaacatcat attcagccca tcacgctgtg gtttgatgat gcgctcagtg
     8701 ccatgcgttc gctgaaaggc attggagcca cgcatcttca tgaagggcgc gacccgcgaa
     8761 tattaacgcg ttcgcagttg cagcgattgc aactggcctg gccacaaaaa caggggcgat
     8821 atcccctgac gtatcatctt tttttgggag tgattgctcg tgagtaaacg ttattttgtc
     8881 accggaacgg ataccgaagt ggggaaaact gtcgccagtt gtgcactttt acaagccgca
     8941 aaggcagtag gttatcggac ggcaggttat aaaccggtcg cctctggcag cgaaaagacc
     9001 ccggaaggct tacgtaatag tgacgcgctg gcgttacagc acaacagcag cctgcagctg
     9061 gattacgcaa cggtaaatcc ttacaccttc gcagaaccca cctcgccgca catcatcagc
     9121 gcgcaagagg gcaggccgat agaatcattg gtaatgagcg ccggattact cgcgcttgaa
     9181 caacaggctg actgggtgtt agtggaaggt gctggcggct ggtttacgcc gctttctgac
     9241 actttcactt ttgcagattg ggtaacacag gaacaactgc cggtgatact ggtagttggt
     9301 gtgaaactcg gctgtattaa tcacgcgatg ttgactgcgc aggcaataca acacgccgga
     9361 ctgactctgg cgggttgggt ggcgaacgat gttacgcctc cgggaaaacg tcacgctgaa
     9421 tatatgacca cgctcacccg catgattccc gcgccgctgc tgggagagat cccctggctt
     9481 gcagaaaatc cagaaaatgc ggcaaccgga aagtacataa accttgcctt gttgtagcca
     9541 tcctgtattt ggt
//
LOCUS       DAPGWD010000163         9366 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPGWD010000163 DAPGWD010000000
VERSION     DAPGWD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.164"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            133..1911
                     /locus_tag="RHX88_003749"
     CDS             133..1911
                     /locus_tag="RHX88_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDV2232394.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1951..2430)
                     /gene="xdhC"
                     /locus_tag="RHX88_003750"
     CDS             complement(1951..2430)
                     /gene="xdhC"
                     /locus_tag="RHX88_003750"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDV2232395.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2427..3305)
                     /gene="xdhB"
                     /locus_tag="RHX88_003751"
     CDS             complement(2427..3305)
                     /gene="xdhB"
                     /locus_tag="RHX88_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDV2232396.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3316..5613)
                     /gene="xdhA"
                     /locus_tag="RHX88_003752"
     CDS             complement(3316..5613)
                     /gene="xdhA"
                     /locus_tag="RHX88_003752"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDV2232397.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6028..6783
                     /locus_tag="RHX88_003753"
     CDS             6028..6783
                     /locus_tag="RHX88_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDV2232398.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6862..6935
                     /locus_tag="RHX88_003754"
     tRNA            6862..6935
                     /locus_tag="RHX88_003754"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6894..6896,aa:Gly,seq:ccc)
     gene            7169..8779
                     /locus_tag="RHX88_003755"
     CDS             7169..8779
                     /locus_tag="RHX88_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232399.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAQTHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPVQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAINTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPTNLSKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            8792..8911
                     /locus_tag="RHX88_003756"
                     /pseudo
     CDS             8792..8911
                     /locus_tag="RHX88_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            8911..9276
                     /locus_tag="RHX88_003757"
     CDS             8911..9276
                     /locus_tag="RHX88_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232400.1"
                     /translation="MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLGEIFPLISSELAGMALMPETERLIGEWLSLSSSPLNLKHPELKYNRL
                     HVGKVFDGVLSPAQPLIRI"
BASE COUNT         2554 a         2114 c         2298 g         2400 t
ORIGIN      
        1 aatttcatta gaaaatgata aaaatcttat gtttttatca aagccaattt cgcctgagtt
       61 aatatcatca tgatctgtga accatcaacg tcttcaatga tcgactttta agtaagtctg
      121 gaaggtaaag ctatggagct tgctactacg cagtcagtat tgatgcaaat tcaaccgaca
      181 attcagcgtt ttgccagaat gcttgccagc gttttgcagc ttgaggttga gatcgttgat
      241 gaaaacttgt gtcgcgtcgc cggaacgggc gcgtatggga agtttcttgg tcgccagttg
      301 agcggcaact cacgcctgct ccgccacgtc ctggaaacga aaactgaaaa agttgtgaca
      361 cagtctcgct tcgatcccct ttgcgaaggt tgcgatagta aagaaaattg ccgtgaaaaa
      421 gcatttctgg gtacgcctgt cattttacag gatcgttgtg ttggggtgat aagtttgatt
      481 gccgttaccc acgagcaaca agagcatatc agtgataatt tacgcgaatt ttctgattat
      541 gttcgccata tatccaccat ttttgtttcg aaacttctgg aggatcaggg gccaggagat
      601 aacatcagta aaatattcgc gaccatgatc gataatatgg atcagggcgt attagttgtt
      661 gatgatgaaa gtcgggttca gtttgttaat cagactgcct taaaaacact tggtgttgta
      721 caaaataata ttattgggaa acctatccgt ttcagaccat taacatttga gagtaatttt
      781 actcatggac atatgcagca tattgtttcg tgggacgata aaagtgaatt aatcattggt
      841 caattgcata acattcaggg ccgacaatta tttttaatgg catttcacca atcgcatacc
      901 agtttttctg tagcaaatgc acctgatgaa ccacatattg aacaattggt tggcgagtgc
      961 cgtgttatgc ggcaattaaa acgactcatt agccgtattg cacccagccc atccagcgtt
     1021 atggtggttg gtgaaagcgg cacgggtaaa gaagtcgtcg cccgagcaat ccataagttg
     1081 agcggaagac ggaataaacc ctttattgct atcaactgtg ccgcgattcc ggagcagctt
     1141 ctggaaagcg aactgttcgg ttatgttaaa ggcgcattta ctggcgcttc tgccaacggt
     1201 aaaacagggt tgattcaggc ggcgaatacg ggcacgctgt ttctcgatga aataggtgat
     1261 atgccattaa tgttgcaggc taaattactg cgcgctattg aggcgcgtga aattctgccg
     1321 attggtgcca gtagcccaat acaagtcgac attcgcatca tttctgcaac taatcagaat
     1381 ttggcccagt tcattgccga aggtaaattc cgcgaagatc tcttctaccg acttaatgtt
     1441 atcccgataa ctctgccacc gctgcgtgaa cgtcaggaag atattgaact attggtgcat
     1501 tactttttac atctgcatac ccgtcgtctg ggatcggttt atcctggcat tgctcccgat
     1561 gtcgtcgaaa tattgcgtaa gcatcgttgg cccggaaacc tgcgcgagtt aagcaatttg
     1621 atggaatatc tggttaacgt agttccttca ggtgaagtta tcgacagcac gctattgccg
     1681 ccaaatctgc tgaataatgg cacaacggag caaagtgatg taacagaggt cagtgaggcg
     1741 cacctgtcac tcgatgatgc gggcggcacg gcgctggagg agatggaaaa gcaaatgatc
     1801 cgcgaggcgc tttcacgtca taacagcaag aagcaagttg ctgatgaact gggcatcggc
     1861 attgctacgc tctatcgcaa gattaagaaa tatgagttgt taaacacata acctcagtga
     1921 atccggcctg aattcaggcc ggatatcctt ttacttcgtt ttctcgcaat ccagaactgt
     1981 atttacaatc atctgatacc ccgtgcagcg acaaagattt cccgccagtc cgcgacgaat
     2041 ctccgtaatg gttaatggct tctcgcgtgg tttcgccagc attgccgtgg tagccataat
     2101 caggccaggc gtacaaaacc cgcactgcac tgcgccggat ttcgcataag cctgctgaac
     2161 atgagaaagt tttccgcctt tcgcttcacc ttccagcgtg cggatctctt ttccttcagc
     2221 ccaggcggca aggtataagc aactgtctat tgctgtgccg tcgaccaaca ccgtacaggc
     2281 accacattca cccacgcagc acccttgttt gacacttagc agtccttgtt cgcggagtaa
     2341 ttccgagagc ggcgtgcctg gtgcggcgtg aagctgaaaa ggcatcccgt taatggtgca
     2401 ttcgatggta attgtttcgc tgtgattcat tgcaattttc cccccgccgc ggcgacggct
     2461 tcgctaatca cttttttggt catcgtctgg atgagatgca gacgaaactc tttactggcc
     2521 cgccatgaag aacgcggggc gacatcttgc aggacagatt cgctgatagc ttccagcgtt
     2581 tgcaggttta atggcgcatt ttgtgcagtc tgttcggcat gttggcagcg aatcggcgtt
     2641 ggcgcggcaa caccaaatgc caggcgtaat tcgctgaaat tgccgttatc cagtcggcaa
     2701 tgtgcggcgc agccaatcgt tgaaatatcc attgcgtcgc gcatggcata tttaaaatgc
     2761 gcgctgcccg cgtgttcttt cggctgtggc ggaaaatgaa aggcgacgag gatttcgtca
     2821 tgctcaagag acactttgcc cggcccggtg tgaaagccat taatcgggac gaaacgaaca
     2881 ccgcgcgggg agtggatctc cagtttcgcg tcataaatta gcgttggcgt ggcagaatct
     2941 gcgctggtgg caccgttgca aatatttcca ccgtaggtag cgacgttacg gatctgcggt
     3001 ccagcaatgg acgtggccgc agcacataac gccgggagat gacgttgagt tataggatct
     3061 tctattagct gggtaaatgt cgttgcagag ccgatacgta gcgagccatc ttccgccagc
     3121 gtaattcccc gcagctccgc cagattatga atatcaacaa tatggcgata acggtcattg
     3181 tgatggtgga gctgaatcag tacgtcagtg ccaccggcga gcagtttggc ctgcgggttg
     3241 tcagccagca ggttgatggc atcggcaagg gttgctgcgc gatggtaaga agcaaaatca
     3301 aacatgatgt tatcctcaaa tcaatcctgc cagatggaac tcttcatata accgttttgg
     3361 cgtcagcggc agtgtattga ttgcaacacc ggtagccatc ttcaccgcgt tacgaatagc
     3421 agcggcaaca ggaattattg gtggctcacc cagtgactta tgtccgtatg cggattgcgg
     3481 ctcattgatt tcgacgaacg cgctttccag ttgtggcaga tccggcatgg tcggcatttt
     3541 gtaatccagc agattggggt tacggaccac gccgctttta gcatcgatga tcatctcttc
     3601 aaatagcgcc cagccaatgc ccattcccat tccgccgtgt acctgacctt ctgccagcag
     3661 tggattaaga atatgccctg aatcatgaac gttgaggatg cggttgatgg tgactttgca
     3721 cagcgcaata tcgaccgtca gatcaacaaa ggtacagcca aacgccggtg ggttagtggt
     3781 ggttttgatg gagctttcag cagagagctg cccgccgcgt tcagggtggt agaaagcgtc
     3841 catcgccaaa tcttttaacg acattaacgg ctcttccggt cgttcaacca gcacgatatg
     3901 gccttttatc agggtcagat tcatcgctga ctgatgtagc atgactgcgg cgtgagcgat
     3961 gattttctct tttaataata gtgccgcact gcgcagcgca ggcgcggcaa catagctctg
     4021 gcgtgaggca aatgcgccgg gatcgaacgg cgtaacgtcg gtatcttgag ttgaaataac
     4081 gcgaacgtcg ctgaccggaa cccccacggt ttctgccacc atttgcgaga agacggtgtc
     4141 ggcaccctga ccgatttccg tcgcgccgct ttgcacgttg atggttccat cctgattcat
     4201 cagaaggcgc gcgcctgcta tttctacgcc gacaggccag gtgttagagg tgtagctaaa
     4261 acaggcgacg ccaacgccgc ggcgcaaatt gccttgctgg ttctggcatt ctgcacggcg
     4321 tttttcccat tcaaagattt tccggccttt ttcaagacac tccggcaacc ctgcgctgta
     4381 aatacgtttg cccgtgagcg gattagcatc tccttcgcgg gcggcgttgc gtaaacgaat
     4441 ttcaacagga tcaataccta acgctgtcgc ggcgtcatca agcatagact caacggcaaa
     4501 tacgacttgt ggcgcgccat aaccacgcat cgcaccagcc gaggggaggt tggtatagca
     4561 ggtctttgaa ctgtaagcgt aggcacaacg aggataaagg taagcgactt tattcccccc
     4621 cgcagaagcg atggagtgcc cgtgagatgc ataagcgccg gtgttagaca gaacatccag
     4681 actataacct ttcaatgttc cgtcgcggtt cacgcccatt tgcccgtcaa tggtaaaagc
     4741 gtggcgggta cgggttgcga ggaaacactc ttcacggcta agggaaactt tcaccggaat
     4801 gccgccaagc ttgctggtca ggaatgccgc cattggctct tccagtacat cctgtttatt
     4861 accaaaaccg ccaccgacaa atggtttgat gactcgtacg catgaccagg gaatatccag
     4921 cgcctgacca accacgcggc gaacaatgtg cgggatctgg gtgctggaaa cgatggtaat
     4981 tcgcgagtca tcctccatcc acgccagcga tgttacgctt tccatgtgac aatgttgaat
     5041 aacgggggtc tgatagtgcc cctgtacctg gtagtcggcg gcatcgattg tttgttggac
     5101 attacccgtc gacatcgtgc tttgtttcag taaattgcca ccgttatgga ttggtgcagc
     5161 gtcttctgcc agcgccgctt ctggcgtggt gataacgggt aattcttgcc actcaatgct
     5221 gaccaattgc gccgcttttt ctgccgtgag ttcatcgcgg gccacgacga tggcaacggc
     5281 gtcaccatga tgacgaacat ggcgagttag cagtgcgcga tcggcggtat cgcgcttgtt
     5341 ttcgtcaagt gtccaggcat gcccagcagt agcaaatgga atatcaggca catcttccca
     5401 ggtaaaaatc gccagtacgc ccggcaaact tctggcttgt tcatcattaa tacttacggc
     5461 ataaccatgt gcgatagggc tacgtacata tttcgcataa cacatgcccg ccataacata
     5521 atcgtcagta tatcgtgccc gcccggtgac cttagcaatg gcatcgacgc gcatgcatga
     5581 ttcacccgta gcggttgctt cccgcgcttc catagaaatc ccctcgattt gaacatttgg
     5641 ttcttatggc aaagatttag tctaagcagg caagatttac gccagaaacg acgataagcg
     5701 ttaggggagg taggaagtgt gatgacgtta aaaaaacaca gatatgatac gaaacaggac
     5761 tctgaatagg ggcagtgata cgtatcaata tgagtggcga taaaatcaat gtgataaatt
     5821 gggattatta cgcatcagga tgggaaatag ggtaaaaagt caggaagttc gatacctcta
     5881 cagcgaatat tttgctgatg atacttccct ttgccaaatg aaattaacct ctttctctat
     5941 taccacgttt ttccagaagc aagagattgg gtatcaagag gctggctgct atgataaggc
     6001 gtcttgtttt ttaagcgagg aaagattttg agtgcgggac gcctgaataa aaaatctctg
     6061 ggtatcgtga tgttgttatc ggttggactg cttttggcgg gctgttcggg tagcaaatca
     6121 tccgatacag gaacgtattc cggctccgtt tacaccgtga aacgggggga tacgctatat
     6181 cgtatttcgc gcaccacggg aaccagcgta aaagaactgg cgcgactgaa cggcatttcc
     6241 cccccttaca ccattgaagt tggtcagaaa ctaaaactgg gtggggcgaa aagtagcagt
     6301 agtacacgta aatcaaccgc caaatcaacg accaaaaccg catcggttac accgtcatca
     6361 gcggtaccga aatcttcctg gccgccagta gggcaacgtt gttggttatg gccaacgaca
     6421 gggaaagtta tcatgccgta ttcgacagca gatggcggca ataaagggat tgatatctca
     6481 gctccacggg gtacacctat ttacgccgcg ggtgcaggaa aggtggtgta tgtgggcaac
     6541 cagctgcgtg gctacggtaa tctcatcatg attaaacaca gtgaagatta cattacggct
     6601 tacgcccata atgacacgat gctggtaaat aatgggcaaa gcgtgaaggc tgggcaaaaa
     6661 atcgccacta tggggagcac ggatgcggca tctgttcgcc tgcatttcca gattcgttac
     6721 cgtgcaacgg caattgatcc gctacgttac ttgccgccgc agggcagcaa gccaaaatgc
     6781 tgatggcgaa ttaatcagca gtcagcagcg cggcacttgc taaggagagc gtaaggttta
     6841 taatgcctta cgcatctcga agcgggcgta gttcaatggt agaacgagag cttcccaagc
     6901 tctatacgag ggttcgattc ccttcgcccg ctccaattta tcttcacctg atcaatctgc
     6961 catttttgct gtatctgatt ttctatatta tcttaattca atcgcttcag acttttcgca
     7021 atcgccgtaa tacttcccca ttttgttgta aataaaaagc gtttacgcaa tgcaatcctt
     7081 ttgtaacaac ccaggtaaaa caccttattt tcaagttgtt aaataagtgg ttatctacgc
     7141 ttttaacaaa cgattgatgg aatattttat gaaagggaaa tctgcattaa ctcttctgct
     7201 cgctggtata ttttcgtgcg gcacatgcca ggcgacaggg gcagaagtga cgagtgaatc
     7261 tgtatttaac attcttaatt ccacgggggc agcaacagat aaaagttatt tatcgttaaa
     7321 tccagataag tatcctaatt atcgtctttt gattcactca gccaaattac aaaatgaaat
     7381 aaagagccac tataccaaag acgaaattca aggattgcta acattaacag aaaacacacg
     7441 aaagttaacg ttgacggaga aaccctgggg aacgtttatt cttgcttcta ctttcgaaga
     7501 tgacaaaaca gcggcacaaa cccactatga tgctgtctgg ttgagggata gtttatgggg
     7561 ctatatggca ttagtatcag atcagggaaa tagtgtagcc gcaaaaaagg ttctgctcac
     7621 cctgtgggat tatatgtcta cgcccgacca gattaagcgg atgcaggacg tcattagcaa
     7681 cccaaaacga ctggatggag ttcccgtgca aatgaatgct gtgcatatcc gatttgacag
     7741 caactccccc gtgatggcag atgtgcagga agaaggcaag ccgcagctgt ggaaccataa
     7801 acaaaatgat gcgttaggtc tttaccttga tctccttata caagcaatca acactggtac
     7861 tattaacgct gaagattggc aaaaaggtga ccgattaaag tcggttgcgc tgctgattgc
     7921 ttatctggat aaagccaatt tctatgttat ggaagattcc ggagcatggg aagaagatgc
     7981 ccgattaaac acatcttccg ttgcactggt gacctctgga ctggaacgtt tgtcaaacct
     8041 actctcgaag aaagactctg tttttgtctc tgatttacta cgcgaagcta aagtcaatga
     8101 actggatgaa actctttcaa caacgaggtt gaaccacctc atagataaag gttatgaaag
     8161 aataacgtta cagcttgatt tagggggaga atccccaggt tatctggaaa aagataaaca
     8221 ttacagagaa gccgatgcgg ctttacttaa tgttatttat cctacaaatc tttcaaagat
     8281 aaacaccaga agaaaagagc aagttctaaa aatagttaaa aaactagcag gtccgtatgg
     8341 tataaaaaga tacgagaagg ataattatca gtcggcaaat ttttggttca atgatattaa
     8401 aaccgatacc gatcaaaatt cgcatgcaaa aagagaaaag agcttcattc catcaacaga
     8461 agcagaatgg ttttttgact catggtatgc aaaatcagca gcaattgttt ataaagaatc
     8521 aagaaaggaa gagtatttga atgactcagt acaatttatg aatagatcat tggcccaaat
     8581 aacaggcgag aatatgattg gtgcaaatgg caggagcgta ccagaaatgg cacttcccga
     8641 aagctataac tatatccaca agtcgggaac tttgcatgaa gcgccaagcc ctatcattcc
     8701 gctaaattgg tcgaaagctt caatgactct catgttgaaa gagatgtcca accttatcaa
     8761 tgatgaggga attaaatagt tttcataatg agttacagca caacgttggg tattgactga
     8821 gcaagatgaa cgacaaggac aacgacatca gccttatgag gagcaagaaa atgaaagcaa
     8881 attcgaaaac gatcagaagg atgaaagtta atgttcggtc tgaggaaagt aaatcgaaac
     8941 actcatacgt ttgaaaccac ttttcaaaat tggaaagaaa atggcgaaga tgttgcatta
     9001 ttaatgcccg aatttagtgc taaatggtta ccgatagcag aagagagtgg cagctggtct
     9061 ggatgggtat tgcttggaga aatatttccc ttaatttcat ctgagctcgc tgggatggct
     9121 ttaatgccag aaacggaaag gttaataggg gaatggctca gtttatccag ttccccgtta
     9181 aatctaaagc atcctgaact aaaatataat cgtttgcacg taggtaaagt atttgatgga
     9241 gtattgagcc ctgctcaacc actaataaga atatgaacag gggaattgaa tctttggtta
     9301 gataaagtca cagtctgcca atacggaaac gctccagcgt tagacaaaaa atcattatat
     9361 tggtcc
//
LOCUS       DAPGWD010000164         9225 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPGWD010000164 DAPGWD010000000
VERSION     DAPGWD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.165"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(861..1463)
                     /locus_tag="RHX88_003758"
     CDS             complement(861..1463)
                     /locus_tag="RHX88_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232401.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(1557..1763)
                     /locus_tag="RHX88_003759"
     CDS             complement(1557..1763)
                     /locus_tag="RHX88_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV2232402.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            3290..3859
                     /locus_tag="RHX88_003760"
     CDS             3290..3859
                     /locus_tag="RHX88_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV2232403.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            4119..4520
                     /locus_tag="RHX88_003761"
     CDS             4119..4520
                     /locus_tag="RHX88_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232404.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            4508..4918
                     /locus_tag="RHX88_003762"
     CDS             4508..4918
                     /locus_tag="RHX88_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDV2232405.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            5202..6821
                     /locus_tag="RHX88_003763"
                     /pseudo
     CDS             5202..6821
                     /locus_tag="RHX88_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            6901..7193
                     /locus_tag="RHX88_003764"
                     /pseudo
     CDS             6901..7193
                     /locus_tag="RHX88_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(7233..8267)
                     /locus_tag="RHX88_003765"
     CDS             complement(7233..8267)
                     /locus_tag="RHX88_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HDV2232406.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIIDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(8270..9202)
                     /locus_tag="RHX88_003766"
                     /pseudo
     CDS             complement(8270..9202)
                     /locus_tag="RHX88_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333640.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT         2322 a         2227 c         2447 g         2229 t
ORIGIN      
        1 tgtggccagt ctttctggct ggcggttcag gggatgtttt ctggttatcg tgtaccacaa
       61 agcagggata ttgccccgat agtcacattt cagattgatc gcggtctgaa gatgtggcac
      121 tgaggtaacg tgctggtgtc actggtattg ctgaagttat ggtttttcgg ggtcaggtga
      181 gcctgttgtg tgtatgcttt ttgttcaggt cagaggggga ctgtgtggac agcggctttt
      241 gtctccgggc attcacgcct gaacgatgaa tgcgattttc agcaatattt gtctttgtga
      301 cactctctgg ttgagaagct aatgcgcgcc cctgtttcca cagagaatga ggcttgccat
      361 cagccgaaac atcagtattc cacgccatac agacggcgcc tgaccatgtg ctgatgcagg
      421 taaaatgttt cactacaact gttcgggttg ctatgcaacg aaacagcgat tctcacgatc
      481 tttaactgca acgtttttcc gcctgaaagc tgactgcctc ctgctgaggt gggaggctat
      541 gattcagtgc gtttatagtc tccggggtat gcatgtaccg gagcatacca tttctggtgg
      601 ataacaagcc ggcacattca gactgcagtg aagatggagg cacctggttg tgaagaacgt
      661 ttctgcccgt ggctgcttcg catcatcaat ggtattcata cccgctcaga aatgaccggg
      721 tgctgaagcc ctccgttatc gcgcagtata atgacgtata tgatctgtgg acgagtgagc
      781 ggcgtgcctg atgtacgccg ctattactgt attttaccac agggctggtt ttctgctgcc
      841 agccctgtgg ttctcagaca tcaactgtga gcggcatcaa ccgatgtacg gtatgccata
      901 agcacgtact gatccatcag atttgtcagt ggttcactct gaccctgacg tgcaatgatc
      961 cattgctcca gatctttttc tgatttgtgt ttcaggtttt tcgtccagtc gtcaaggctg
     1021 tttccggtca ttaagtcaag ccagcagagc cagaccagtt tgagacgtaa ctccgtgccg
     1081 ccaaactgac ggaagaggct gatcagtttg tttgccagag aatgttgctt caggcgctca
     1141 cattcagaca gcaggatccc ttccagcatc ggatgatgaa ccagcaggtg ttcgctggtg
     1201 cataaattca ccaccagatc atcatccagg gacggggagc tccgtttgtc cggaccataa
     1261 atcagccggt gcagactctg gggaacgatg gcaatctccg gtttttcgcc attgaatgac
     1321 acccacccgg gcgtcagatt atggaaatag tagatattcg gatgacacac gccccatgat
     1381 acggcgtgcc gggtgaagcc ggtcagccat ttctggtagt tttcatggag cagctcgtaa
     1441 cgggtctgat actgttgttt cattttcata ttcctcacgg gggataacag gcagcagagc
     1501 gccgccggca ggccggagat tgcgtctgct gtgggcgaag ggttagtggt taaaaattac
     1561 ggacgggact gtgcaatacg cgcctgcagc caggcttcca cttcactttt cagccagcgg
     1621 gagctgcgcc ccattttgat gggcgcagga aagcccccat ctttgatgag tttgtcaaac
     1681 cacttatcgg ttaggccggt cagttgagtg ataaacgcca tgtcgaccat ctggtcatcc
     1741 atcagcgaaa ctggggtagc catgagaatg ttcctccgga tgttgttaaa ccagccggtg
     1801 tggtcatgaa aacagcgcgc caccttcacc ggcataacaa ccggggagaa acgtaaaaaa
     1861 taatcgggaa tgcggctgtc cggagataaa cggggggatt gcgatggcag agccgtagcg
     1921 gaatcccgga ggggggtgtt gcgaaaagat gcttccgcac gcacgcgcga taaagagggg
     1981 atagttttcg tgaggacagg gagagaagca ggcgttctga taattgcaga gttgttttcg
     2041 gaggtggtta cagtgagtca gcccgttcct ggcgagcagg agactgatat caccgatggg
     2101 ataaggttgt ctgattggta ttataaatat aagaagtcat cagagagtaa cgtaaaggat
     2161 ttgccagaat aacctaagag agtaaatctc tccggtaaca gagcgctgtg aagcattctg
     2221 tatcagagtg cagatacatg aacgctgatg cgtaaaatca gcagtgacac gccagctccc
     2281 cctatgccca ttcctgtata agacatgaac tgagtgtatt aatgcttctg ccctgagact
     2341 gagccggtga gtgaggggct gagaaatccc tcttgacaca gctctaacca gaaatacttc
     2401 cccggttgtt gcggtgcgtc cggatccccg ataacggaac actaaggctg tattgcggga
     2461 caccgggtaa gtagcagccc gtaaggcgtt ctgtcaacgc cagaaaaaat gatttcccac
     2521 acgcgttata gactcactcc tgtggacctg gaagcccgta actctgcggt tgcccactga
     2581 ctgacggaac tgaccgcaaa ttttacgagt gcgtgctggg cttcagtctg gtcacctgag
     2641 cgttcggtaa cggaggcgcg ggcggtaata ggctgatttt caggtattac cggcttacag
     2701 cgtgtggttg atattgaggg gctgtgagtt tcgcgtattt gtcagaaacc cagacgagtt
     2761 ttcctgtatg ggacgaaata aggcagcggc aatacctgga aaaatttaat atggtatgaa
     2821 ggagaacctg tcggttacgc ttaatatacc cggtacactg tcagctcagg ggatacatga
     2881 accgttatct caccagctac acagtatata cacactgacg ctgacagtag tcagcaggac
     2941 cacaataatc agtgccagca gtatggtaac gatacggtct gtattccggt aatagccgaa
     3001 ggggtcgaca gaaccacaat acgggcaaca cactgtatta tgagtaatcg cgtttccaca
     3061 gtccctgcac ttctttttgc gtatcaccat cactccctcc tcacagcctt acccgtaacc
     3121 ggttttttac attaaaaaaa caacgctgaa aattcaaccg tcagtccgga gacgaccgtt
     3181 cgggttatca cagagtgcct gagacagtgt cctgccggag gtcacttcag ggactctgcg
     3241 cattttttac gacgtggcta ttccgtatga accatacgga gatttaacca tgacctacaa
     3301 atacaacccc ttctggcagc aacgtattcg tgagacggtg cggcacgcac tgaatgttca
     3361 tccccgcctg acggcattgc gggttgacct gcgtttcccg gatgtaccgg cagcaacgga
     3421 cgcagctgtg atatcccgct tcatcaatgc cctgaaagcc cgaatcgacg cttaccagaa
     3481 acgtaagcat cgggaaggta aacgcgtgca tcccaccacc ctgcattacg tctgggcccg
     3541 ggagtttggg gagtgcaaag gtaaaaaaca ctatcacctg atgctgctgg ttaaccggga
     3601 tacctggtgt cgtgccggtg attaccgtgc tccgggatca ctggccggga tgattaaaca
     3661 ggcgtggtgc agtgccctgg gagtggatgt cgggtgccat gccacgctgg tgcattttcc
     3721 ggcctggccg gcggtgtggc tggaacgcga tgatgacact ggctttcagc aggtgctgga
     3781 acgtgctgac tatctggcga aggaacatac caaagctcac tgcaccggtg agcgcaattt
     3841 tggctgtagc cggagttgag cacgactggc gcgccaatat ccggcaaacc aaaagtatgg
     3901 gttgttgtct ggcggcaacg atgtatccac tcgcccactg actgtttttt acgtcagtgt
     3961 tccattgtgt gacaaccaca gtgctgtaac agcaaccagc ctgctttacc caaactccag
     4021 tccagatttt aactgaaccg ccattcacac cctgaccacg ctgcccgtac cgggacggct
     4081 gttgcctgcg tgtcgtctgg cggctgagga atatgactat gtacgcaaaa tcctttatcg
     4141 ctcttgatgg caacggacgt ctgacgggcg cccgtactgc acaggccgca ccttatgcta
     4201 actacacctg ccacttgtgt ggcagtgcac tcagatacca tccgcaatac gacactgaac
     4261 ttccctggtt tgaacacact gacgacaggc tgacagagca cggtcaacag tgcccttatg
     4321 tcaggccgga gcgcagagaa atacagttga ttaaacgtct gcagcaattc gtaccggatg
     4381 ccttacccgt ggtgcgtaaa gccagctggc actgcagaca atgtcaccac gattattatg
     4441 gggagcggta ctgcacacac tgccagaccg gaggattcag tattccccgg acaactcagg
     4501 aggaaatatg cgaattctga actgttatat ggcgaatgac agcaaaggcc attttgttac
     4561 ggcgaaagaa gcagcgaagc acaaccgcca ggacgttttg tgctgtgtgt cctgtggatg
     4621 cccgttaaca cttcagcggg gcaatgacgg acaaccaccg tggtttgaac atgaccagat
     4681 gactgtcgct gagaaaatcc tgctgcgatg cacctggctt gacccggcag agaaagaggc
     4741 ccgtcgtttg catctgcagg gtatgacggt tccggattat acggtgaagg tgagaaagtg
     4801 gttttgtgtg atgtgtgacg aagattatga aggagaaaag tgctgcccgc gttgtggtac
     4861 cggggtatac agcagggagg gggggctgca ggaaggcaac tggaaggata ggaactgagc
     4921 ggttcagtcg cggaaatcag ttgcagagct gtaatgatat ctccgggagt gctgcgttgg
     4981 taggggcatc ccgggataga gaaattttag cactttgccc tgaatgattg tagacgttcc
     5041 gagtactcgt accggtctga gttattacag ttagatattc actttttctc ccgcatactt
     5101 cacatctgtc aacgacggat gaaaagtggt tcttccgcaa cctgggtgga agaaaatgga
     5161 tgaaactttc tggcgaacaa ggatctaagc tgttttaagt tatgggcaac gcaatgcact
     5221 ctcttaaaac acttctacag ttaccttgcg gatggcgatg cagtcgacaa attattagct
     5281 ctgacggtat aaccctccat ctccacggaa aacgcaaaac agcacaatgt cctgaatgct
     5341 ttaagcgtag cgactctgtt catagttgtc gtcggcgccg gatacagcat ctaccctgct
     5401 cagggcagac gctatggctt gtattttccg tccgccactg gtactgccgt aacccggctt
     5461 gttcacgtaa aatttttgcc gagtcgcttg ctcccttcgc cggttcacac cagcagtctt
     5521 cacaggcgtt acaaaattta caacgtcaac tgggattaat agctggaggt gaggctggaa
     5581 aacgggctgc aacggcagcg ggtctccgtt gcagtgctga tactcttctt cgcagggtta
     5641 tcaatacccc ggagacgaaa cagtcaggcg cgcctcatgt cggtattgat gagtgggcgt
     5701 ggcatcgggg ccactgctgc ggtatgttaa tagtcaatct tgatactcac cgtcccctcg
     5761 tcctgcttcc cggccgtgat cagcgtacgc tggcgacctg gttcagaaaa tatccggaaa
     5821 tacaggttgt ctcgcgtgat cgcagtggag tctatgcgac agcagcacgt gaaggtgcac
     5881 ctcaggccag acaggtggcc gatcgatggc acctgctaaa aaatattggt gatgagcctg
     5941 aacgaatgat gtacagacat atgcctctga tacgtcttgt tgtcagagag ttatcactga
     6001 agaaatcacc tgagccagaa atatctgtgc ctgtagcatc gctccgtcgt ctggaacgcc
     6061 ttaaacagca catccgcaaa aaacgacatc agcgttggac agaggttatg gccctgcata
     6121 acaagggatg tagtttcagg gaaatatccc gtattacagg cctgtcgcga gtgacagtca
     6181 gtcgctgggt gggttcagga acattccctg aaatgtcaac taggcctcca aagcgagggc
     6241 ttctggaccc atggagggag tggttaaaag agcaacgaga atgtggtaat tataactccg
     6301 gccggatatg gcgggaaatg gtggccaggg gggttacagg cagtgaaacc atcgtcaggg
     6361 atgctgttgc caaatggcgt aaaggctgga tcccaccggt tactactgcc gcaagacttc
     6421 cttcagtgtc ccgggtaagc cgctggttga tgccctggag aataatcagg ggtgaagaaa
     6481 attatgcttt ccgatttatt agtctgatgt gtgaaaaaga accggagttg aaaatagcgc
     6541 agcaactggt actcgagttc taccgtattc tgaaaaccta aaataaatca cagctaagca
     6601 gctggttcac tcgggtccac gaaagcggct cagcagaact tcggtgcgta gctgcgggga
     6661 tggaagctga tgctgcggct atatgtgagg caatcagcag tcgctggagt actggtgttg
     6721 tcgaaggtca tgtaaatcgc ctgaaggtgc tgatacgcca gatgtatggt cgagccggac
     6781 ttgaactact taggcggagg gtaatgagtc cactggcatg aaaatcttca cccaggttgc
     6841 ggaagagcca cttttgcact gttcgtgaca gtatggcctt tatgtactca ctttgttacc
     6901 ggtagctgga tcatcactac cgtgctgcaa atggtggtga acagcatcaa ttgtggaatg
     6961 aatgttgccg aagcgaccaa tgcgcctcgt ttccactatc agtggttgcc ggacgagctg
     7021 cgtgttgaaa agggtttagt ccggatacgc tcaagctgct ggatgcaaaa ggtcagaaag
     7081 tggcgctgaa tgaggcgatg ggcagcacgc aaagcattat ggttgggtcg gatggtgagt
     7141 tatacggcgc atccgactcg cgcttggtgg atgatttaac ggcggggtat taattgacat
     7201 cccccggcgc aatgtgccgg gggataatgg ttttatgcgg tcaataatcg tccgggatta
     7261 tcgatacaga gttttttgag gatcgtttta tcgacgccgc gctccgccag ataggccaga
     7321 aaaacatctg gaacaaatcc ccagccatta ccgccatttt tagcccacat ttgtttaagg
     7381 aagacatcgt ggctgagaac aagctgatcg gcgtatccca actcaatgag atgagcgacg
     7441 gcatcggcag tctcctgcac ccctggcgct attccctctt tcgggaaggt aatgtctagg
     7501 ccaatcatgt cgaattccag ccagacacct ttatcaagca ttttccgctg atacgccacg
     7561 tcttttcctg acggatccga gtgtgcgaga gagactttat ttggcgagac gcccatttca
     7621 cctaacacaa tgtcgagtac ttcatcaccg cgacgaagcc agcccggcat gtgaatattc
     7681 atcgccacat gaggattgtt aatctgtgcc agcgaggcag cccgcaagct gttatgctcg
     7741 gcttcggtaa atgtcggtga aacacctatt tcaccgatca ttccggcacg aatatccgta
     7801 tcgccaatcc cttgattcaa ttctttatcg atcgttgtcg ccagttcatc aaccgtttta
     7861 tgaattctct ggctttcaaa tttttccagg tagggaccgg aggaagcaac aatattcaga
     7921 cctgttttta atgcgacttc tcgtaatgcc tgcgcatctc gtccgataga ctcagaccct
     7981 gtggcatcaa taatggtgcg accaccgagc gagataaagt tattaatttc gaagatcaca
     8041 tcttcaatag gtttgcggtc catattatca gcgcagcaat aaggatcgtg ttttaatgcc
     8101 cactggattt ctgccgtgac ttttttatca acaagccgtt gtgaaaaagg atagcaagga
     8161 gcatccacaa cagaggaaag atcgttgaac agatgttcat gaggaagtgt tagccccata
     8221 tcttcacggg cgacggggcc cgtcacagtt tgtagataat ctttcatgat tatacctcca
     8281 ttttcttcat tgttttcgag gttctgtatt ccggaatagt ggtaacaatt gcaccgacca
     8341 gtgcaaacag tgttccgatg atcgtgacca gataaacgct attgcccagc gatggaatta
     8401 atacgtcaat caatactgat cccagtaact ggcccgccgt tgaagcaacc cccagcatca
     8461 gtaaccccag acctctgacc aaaatggcca ttaaaccgat agacattagt ccaagcgggc
     8521 cgccaagata catccaccag acatctggta attgaacagt gacgtgccct aatgcgacac
     8581 gaatagctag cgctgtgcct aaaacacaaa atccaacgat gaaattctag gtgattgaaa
     8641 ccagcatgga tcccgtagct tcagctactt ttgcatttcc agcgggctgc catcctgcaa
     8701 gcaaaccagc taagaacgga agaatggcaa gtaagatgaa agaggtagaa tgccactgag
     8761 gtgacacaac aaaaatcgta gcaataaccg caaataatgc accgatgatg cgataagggg
     8821 taaagtattt tttctcgtcg acaccgatac caaagcgatc gcaaagtaaa ccagacagca
     8881 atagcgcgga gatcagtgcg gtttgaaacg ttgcgacccc gagtgcgctg gctgatgcgc
     8941 cttcagaaaa taccaccatc gctccacaga gaccggcaaa ccagttccag agaggaactt
     9001 ttcggttttt aatcagagaa ggaattgccg caaattgttg ccgggtttct tttcgcgcca
     9061 taataataaa aaacatcacg actaaaccac tggcaaagga gataactgcg catgcattac
     9121 catcctgtaa ccagtgacct aattgaccat tcaccgctga ttgcataggg gagagcatac
     9181 ccgcaagaat cgtggcaagc atccataagg gagttgagta cttat
//
LOCUS       DAPGWD010000165         9208 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPGWD010000165 DAPGWD010000000
VERSION     DAPGWD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.166"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(7..114)
                     /locus_tag="RHX88_003767"
     CDS             complement(7..114)
                     /locus_tag="RHX88_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV2232407.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(519..1619)
                     /gene="chaA"
                     /locus_tag="RHX88_003768"
     CDS             complement(519..1619)
                     /gene="chaA"
                     /locus_tag="RHX88_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDV2232408.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1889..2119
                     /gene="chaB"
                     /locus_tag="RHX88_003769"
     CDS             1889..2119
                     /gene="chaB"
                     /locus_tag="RHX88_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDV2232409.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2277..2972
                     /gene="chaC"
                     /locus_tag="RHX88_003770"
     CDS             2277..2972
                     /gene="chaC"
                     /locus_tag="RHX88_003770"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDV2232410.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(3016..3369)
                     /gene="ychN"
                     /locus_tag="RHX88_003771"
     CDS             complement(3016..3369)
                     /gene="ychN"
                     /locus_tag="RHX88_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDV2232411.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPVEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3554..4948
                     /gene="ychO"
                     /locus_tag="RHX88_003772"
     CDS             3554..4948
                     /gene="ychO"
                     /locus_tag="RHX88_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDV2232412.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGERASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4949..5599)
                     /gene="narL"
                     /locus_tag="RHX88_003773"
     CDS             complement(4949..5599)
                     /gene="narL"
                     /locus_tag="RHX88_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDV2232413.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5592..7388)
                     /gene="narX"
                     /locus_tag="RHX88_003774"
     CDS             complement(5592..7388)
                     /gene="narX"
                     /locus_tag="RHX88_003774"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDV2232414.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7727..9118
                     /gene="narK"
                     /locus_tag="RHX88_003775"
     CDS             7727..9118
                     /gene="narK"
                     /locus_tag="RHX88_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDV2232415.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2247 a         2252 c         2359 g         2350 t
ORIGIN      
        1 ttcaccttac ttccggttac gccaccagct gacaatcgct gcggtaataa tccccgccag
       61 gatcggtgct gccaggtcgt gccagaaagt catggcaaac tgcgcgagcg tcatatagcc
      121 gccttgttgt aatgacaaca ttttgcggct attcttgaag tgtctggttt caagattagc
      181 ccccgttttg ttgtcaggtt ttacctctca acgtgcgggg gttttctctt tccagcaacc
      241 aatgccacca gggataaagc ccccgcaaca ttgcgcctca ccggataatt ccggcttggt
      301 gtggatacta cgtctcaatt catcttcact tcatccctga aatgtttgca ataaagagta
      361 cattccggct tttcaacagc tgttgcagtg gtttcgtgca ggttgtggta caggctcgca
      421 attctgatta acgagctgtc caggtagtat gaagaaatac aataagcagg aatttatctt
      481 atctcccccg atttccgtca tcggaggaga tataactgtc aggcaaatat cgtcatcaaa
      541 taggcggcaa acagtgccag atgcgctgcg ccattgagca cgttagtacg tccggtggag
      601 aaggagatat ggcacagcac taaagaggcc accatcacca ccatttctgg cgcaccaagt
      661 gcaaactgta attcgttacc cgtcataaag gcaattagcg tgacgacagg tacggtaagc
      721 gaaatggttg ctaacactga accaaagaac agattcatcg cgcgctggac ctggttgttc
      781 aacactgctt ttaacgcacc taaaccttcc ggcgacagaa tcaacagagc caccaggaag
      841 ccagtaaagg cgacaggggc attcatgctg tcgagcaatg tctccagcgg gctggcattc
      901 attttggtga ctgcaataac ggcaatcaga tggataatca accagatagc atgccacacg
      961 ctgctatggg cagacggttt accgtgatgc gggtcgtcgt catcactgtc atcttcgtgc
     1021 tcgtagacaa acaaactttg atgcgttttg gtctggatca gcaaaaatac gccatacatt
     1081 gccgcagaaa ttaatgctac cagtaacgcc tgaccggttg aaaaattcgc cgcaggcaga
     1141 gccattggaa ataccagtac gattatcgcc agggggaaca gcgcaattaa atactgcttg
     1201 ataccaaaca gattcatata ttgggtggca aacttacgac cgcccaacaa taatgaaaag
     1261 ccaaccagcc caccggtaac aatcataatg attgaataga gcgtatcacg cattagcgtt
     1321 ggcgcggcgt cgccggttgc cattaaagct gaaatcaaac tgacttcaag aataaccact
     1381 gaaaggctaa gaataagcga accgtaaggt tctcccaggc gatgggctaa tacgtccgca
     1441 tgacggacaa cactaaaggc gctacttaaa ataccaataa gcgcaagaag attgatggca
     1501 atgaccactg gtagtgtctg gctgcttccc cacaggaaca gcactaccag cgccagaacc
     1561 gggaaaataa gcgaagtctc cttgtggcgg gtttttaccg cctcttgagc atttgacatt
     1621 atggttatcc ctttgcagat gaatttatcg aaaatgtaaa aaataggtag gaaaaataac
     1681 agaaagtgtc tggatatcgg taacatttta cgaattttta ccccactgtc gattttttac
     1741 tcattgggac ataaaaataa gtattacgtt tagacaatgt ttgttttagc gtttcttaag
     1801 aacagtctga catgaaaatt cttatgtttt ggcaagagta gatattgttg accacactta
     1861 atgttcaact ttgtaaaagg agtcaacgat gccgtataaa acgaaaagcg atctgccgga
     1921 aagcgtaaag cacgttctac cgtctcatgc ccaggatatc tataaagaag cgttcaacag
     1981 cgcatgggat caatataaag ataaagaaga tcggcgtgat gacgccagtc gcgaagaaac
     2041 cgcgcataaa gtggcctggg ctgctgtgaa gcatgaatat gccaaagggg atgatgataa
     2101 atggcataaa aaatcgtaaa accggtcgct tagttaaagc tattcgtgcg gtgttgcctt
     2161 gcaagtggtc cgtggattgc atattgtccc gttagtggtt tcaaaatcag cagtaaaaat
     2221 gtccgaaaga caccaaaaag ttgtcgcagg gaagtatgca gtggcggagg tgtaaggtga
     2281 taacgcgtga tttcttgatg aatgccgatt gtaaaacggc atttggtgcc attgaagaat
     2341 cactcttatg gtcagcagaa caacgggcgg cttcgctggc ggcgacgctg gcttgtcgac
     2401 ctgatgaggg accggtgtgg atcttcggtt atggatcgtt gatgtggaat ccggcactgg
     2461 agtttaccga gtcgtgcacc ggtacactgg ttggatggca tcgcgcattt tgcctgcgcc
     2521 tgaccgccgg gcgcgggact gcgcaccagc cgggacggat gcttgcactg aaagagggcg
     2581 gacgcaccac aggcgtcgcc tatcgactgc cagaagagac gctggagcag gaactaaccc
     2641 tattgtggaa gcgcgagatg attaccggct gttatctgcc aacctggtgt cagcttgatc
     2701 ttgatgatgg acgcacagta aacgccattg tgtttattat ggacccgcga catccagaat
     2761 atgaatctga cactcgcgct caggtcatcg cgccgttgat tgcggcggcg agcggtccgc
     2821 tgggaaccaa cgcacaatac ctgttttcac tggaacagga gcttatcaaa ctgggaatgc
     2881 aggacgatgg gctgaatgat ttgctggtat cggtaaaaaa actgctggcg gagaattatc
     2941 cggatggtgt gttacggccg ggattcgcct gagtaaactt ccctcatagt ggggcgtcag
     3001 acgccccctc aaacattaaa atgtgagcac tttatcggct gacagcgtcc attgcgccag
     3061 ttccacaaga gtaccgattt ccaccccatc aataagagga agtgtgctaa tcccgcgccc
     3121 gtcggtacag gttttgcaca atttcaccgg tacattctga gcggtaagga tctccagcat
     3181 ttgctgaatg ttgtagcctt ccactggttt ttgcccgcgc aacccggctg tgaccgcatc
     3241 agacatcagg aacagacgca gatccagatt gctctcctgc tctcgtaacg caatggccag
     3301 ccgcaagctg ttaaacaagg attcgctccc gtaaggtgcg ccattggcaa cgatcacgat
     3361 tttttgcatt atttactcct gtattcaggg aattagacac tcatcttcta tcttactgct
     3421 tctgcagcgt ctgaccaatc ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat
     3481 acgacaaaag ttgccagtaa tcgttattct ttaaggctat ggtttttcat tattaccgga
     3541 agttaccgac gttttgagcc gtttcgttcc tcgcattatc ccgttttatt tactcttgct
     3601 tgtagcaggc ggtacagcta acgcacaatc taccttcgag caaaaagcgg caaatccctt
     3661 tgataataac aatgatggtc tgccggattt aggcatggct cccgaaaatc atgatgggga
     3721 aaaacacttt gctgaaattg tgaaagattt cggcgaaacc agtatgaatg ataacgggct
     3781 ggatactggc gagcaggcaa aagctttcgc attgggaaaa gtccgcgacg cgcttagtca
     3841 acaggttaat cagcacgtag agtcctggct atcaccgtgg ggaaatgcca gtgttgacgt
     3901 caaagtggat aacgaaggac atttcaccgg cagtcgtgga agctggtttg tgccgttaca
     3961 agataatgat cgttatctca cctggagcca gcttggtctt actcagcagg atgatgggtt
     4021 ggtgagcaat gtgggcgttg ggcaacgctg ggcgcgcggc aactggctgg tgggttataa
     4081 cactttttat gacaacttgc tggacgaaaa tcttcagcga gcgggctttg gtgccgaagc
     4141 gtggggcgaa tatttgcgac tatcggcaaa cttttatcag ccgtttgctg catggcatga
     4201 acagacagcc acgcaggaac aacggatggc gcgcgggtat gacctgacag cccggatgcg
     4261 catgccgttc tatcaacacc tcaataccag tgtcagcgta gaacagtatt ttggtgatcg
     4321 tgttgatttg tttaactctg gtacgggtta tcacaatccc gtcgcgttga gtctgggatt
     4381 aaattacacc cctgtgccat tagtcactgt gacggcccag cataaacagg gtgaaagtgg
     4441 cgagaatcaa aataacctcg ggctgaatct taattaccgc tttggtgtac cgctcaaaaa
     4501 acaactttct gcgggcgagg ttgccgaaag tcagtcgtta cgtggtagtc gctatgacaa
     4561 tccgcagcga aataatctac cgactcttga gtaccgacag cgaaaaacgt taacggtgtt
     4621 tctggcgaca cctccgtggg atctaaaacc tggcgaaaca gtgccactga aattacaaat
     4681 ccgcagtcgt tacggtattc ggcaactgat ttggcagggc gatacgcaga tattaagttt
     4741 aacgccaggc gcacaagcca acagcgcgga gggctggacg ctgatcatgc ctgactggca
     4801 gaacggggaa agggcgagca atcactggcg attgtctgtg gtggtggaag ataaccaggg
     4861 gcagcgtgtc tcctccaatg agatcacgct aacgcttgtc gaaccgttcg atgcattgtc
     4921 aaacgacgaa ctgcgctggg aaccgtaatc agaaaatgcg ctcctgatgc acccataccg
     4981 ctgcttccac gcgagacttg agcttcattt tcttcagcat gtgcttgacg tgcactttta
     5041 ctgtgctttc ggtgatgtcc aggcggcggg caatcatctt gttcggcaaa ccctgggcaa
     5101 tcagcttgag aatatcgcgc tcgcgtgggg ttaactggtt aacatcgcgc tcagtagtgg
     5161 cacggttagc acgcaagctg gcggccagaa caggcgttaa tgcttcgctt aataccattt
     5221 cgccagcagc tgcctgatgc aatgctttca gcagatcttc cggttccata tcttttaaca
     5281 gatagccatc cgcgccgcgt ttcagtgcgg tgaccacatc ttcttcatgg ttagagacgc
     5341 tgaataccac aatgcgccct gagagggact tttcgcgcag tttatccagc gtttccagac
     5401 cgttcatgcc gggcatattg agatctaaca ggatcagatc gggatcaaga gactccgcca
     5461 gttcaatacc ctgttcgcca ttactcgctt cgccaaccac ggtgatatct ggtgccatac
     5521 tgataagctg ttttacgcca gttcgcagca tcgggtgatc gtcaatcagc aggatagtag
     5581 ccggttcctg attactcatg ggtatctcct tggacgtctg tgaaagtttt ttcgggaata
     5641 aaggtgacca ccacttcggt gccacctgat tcacgacggc ggacgcggca atcgcctcgt
     5701 aaactttgcg cacggtcgcg cattattatc atgccgtagt gattgctgcg gatggcattt
     5761 tcaggcacgc cgcagccgtt atcctggacg gtcagtttga cctgattatc gttttgcgcc
     5821 accgtcacca cgacctcact cgcttgcgaa tgtttgaggg cgttacttaa tgcctcacgg
     5881 gcaatttgca acaagtggat tgcctgatgc gaaggcacca gacgaggcgg caattgataa
     5941 tccagcttca ccgggaagcc aaatttggcg ctgtactctt cgcaactcgc ctccagcgcc
     6001 ggacgtaatc caggctcggt gagctgcaag cggaatgtgg tgagcaattc acgcaactgc
     6061 gcccaggatg cattcagttc gttacggatc tgacttaaca gttcgcggct gctttctggc
     6121 agcgcatcgc cctgcatctg taaacaactc acctgcatct tcatacaaga gagagattgg
     6181 gcaatagaat catgcagttc gcgcgcaatg gtggcacgct cttccatcac gatcaactgt
     6241 tgctgacgtt cctgatggcg atccaacgcc agcgtggcgg tgagttgttc aaccagggta
     6301 tccaccagtt gttgttgatc atggctaaga tgacgcccct gcggcagggt cgccagcaaa
     6361 ataccgtact gcgtatgaga gtcagccagc cgccacttca gggttgtgcc gcgatcgcca
     6421 acgggtaata cgccgcgcgg gcagagctgg cagcctttat catcacaagt catatctggc
     6481 tggcaggtaa actcctgatg attctcttca tcatcagtat cataaacccg cagttcgata
     6541 tcacgtagca gggttaaatt ctgtaagccg ttgagtaccg gtgacaggcg ttcacacagc
     6601 ggggcgcggg aatgcaaacg gcggttagcc tgccataaaa atgagaggat ctgattttta
     6661 tgctccagcc cggcggtttt ctcctgaacc cgctgctcaa gtacggcata actttcggcc
     6721 agttctgcag acatattgtt caacgcagtt ccaagcatcg ccatttcgtt gcgcccgctg
     6781 atgtttgcgc gttgggtaaa atcgcgatgg ctgacggcac tcgccattgc cagcagttgc
     6841 cgccacggtt gtagcagtcg cgcccgcaac cagataatag tgaacaccag taaaagtgcc
     6901 ataaataccg ccattacccg atggaccagt accactgtct cgatgcgcat ttccgtggtg
     6961 cggtcaaaac cagataccag ctgatcaagc ccggcaacaa actggctgac atccgctgac
     7021 accgtttctc ggttttgtgc acgcatcagc gcagggatca gttcattacg ccagtaatct
     7081 tgtaaaccct gtaattgcgc cagttgtccg tctcgttctg ctgctcgagt caactcggcg
     7141 ctaaatgccg tttgttccat ctctttaatt aagggcttgt ctttctcgct taatggcact
     7201 gccgccaaca gacggtaact ttgcatgcgc agcgatcccg ctttgttgat cgcatgggcg
     7261 ctgccctgaa cgccttgcac cagccagcca gaaaccgcca tccctgccag tccaatagca
     7321 gtagaaagca acacaataag cgcaacctga ttaaccaggg tgagcggaga gagacaacgt
     7381 ttaagcatgt aaacctcttc cttcaggctt taaatgagca ataaccttaa tgaatgtgac
     7441 gatgcattct ggaatggcag tattctcggc tattggctga agtataccca tacccggaaa
     7501 gagttactcc ttatttgccg tgtggttagt cgctttacat cggtaagggt agggatttta
     7561 cagcaccgtg aaaaatctca taatttttat gaaatcactg tactcactat gggtaatgat
     7621 aaatatcaat gatagataaa gttatcttat cgtttgattt acatcaaatt gcctttagct
     7681 acagacacta aggtggcaga catcgaaacg agtatcagag gtgtctatga gtcactcatc
     7741 cgcccccgaa agggctactg gagctgtcat tacagattgg cgaccggaag atcctgcgtt
     7801 ctggcaacaa cgcggtcaac gtattgccag ccgtaacctg tggatttccg ttccctgtct
     7861 gctgctggcg ttttgcgtat ggatgttgtt cagcgctgtt gcggtgaact taccgaaagt
     7921 cggttttaat tttacgaccg atcagctatt catgttgact gcgctgcctt cggtttctgg
     7981 cgcgttatta cgtgttccat actcctttat ggttcctatc ttcggtggtc gtcgctggac
     8041 ggcgttcagc accggtattc tgattattcc ttgcgtctgg ctgggttttg ccgtgcagga
     8101 tacctccacg ccttatagcg tcttcatcat catctctctg ctatgcggct ttgctggcgc
     8161 gaacttcgca tccagtatgg caaacatcag cttcttcttt ccgaaacaga agcagggtgg
     8221 cgcgctgggt ctgaatggtg gtctgggaaa catgggcgtc agcgtcatgc agttggttgc
     8281 tccgctggtg gtatcactgt cgattttcgc agtatttggt agccagggcg tcaaacagcc
     8341 ggatgggact gagctgtatc tggcgaatgc gtcctggata tgggtgccgt tccttgccat
     8401 cttcaccatt gcagcgtggt ttggcatgaa cgatcttgct acctcgaaag cctccatcaa
     8461 ggagcagttg ccggtactca aacggggtca tctgtggatt atgagcctgc tgtatctggc
     8521 aaccttcggc tcctttatcg gcttctccgc gggctttgca atgctgtcaa aaacgcagtt
     8581 cccggatgtt cagattctgc aatacgcttt cttcgggccg tttattggtg cgctggcgcg
     8641 ttctgcaggt ggtgcattat ctgaccgtct gggcggaact cgtgtcacgc tggtgaactt
     8701 tattctgatg gcgattttca gcggcctgct gttcctgacc ttacctactg acgggcaggg
     8761 cggaagcttc atggcgttct tcgcggtctt cctggcgctg ttcctgacag ctgggctggg
     8821 tagtggttcc actttccaga tgatttcagt gatcttccgt aaactgacaa tggatcgcgt
     8881 gaaagcagaa gggggttctg acgaacgtgc gatgcgtgaa gcggcaaccg acacggcggc
     8941 ggcgttgggt ttcatctctg cgattggcgc gataggtggc ttctttatcc cgaaagcgtt
     9001 cggtagctcg ctggcattaa cgggttcgcc agttggcgca atgaaagtat ttttgatttt
     9061 ctatatcgcc tgcgtagtga ttacctgggc ggtatatggt cggcattcta aaaaataaat
     9121 cttaatattt gattatcctt tgcgcggcat gatgtcgcgc tttttttatg cgtcatttag
     9181 ttacaacata ctcatattat atggctta
//
LOCUS       DAPGWD010000166         9196 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPGWD010000166 DAPGWD010000000
VERSION     DAPGWD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.167"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..482)
                     /locus_tag="RHX88_003776"
     CDS             complement(<1..482)
                     /locus_tag="RHX88_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDV2232416.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     "
     gene            complement(678..1016)
                     /locus_tag="RHX88_003777"
                     /pseudo
     CDS             complement(678..1016)
                     /locus_tag="RHX88_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2533..3078
                     /gene="cobU"
                     /locus_tag="RHX88_003778"
     CDS             2533..3078
                     /gene="cobU"
                     /locus_tag="RHX88_003778"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDV2232417.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            3075..3818
                     /gene="cobS"
                     /locus_tag="RHX88_003779"
     CDS             3075..3818
                     /gene="cobS"
                     /locus_tag="RHX88_003779"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDV2232418.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            3830..4909
                     /gene="cobT"
                     /locus_tag="RHX88_003780"
     CDS             3830..4909
                     /gene="cobT"
                     /locus_tag="RHX88_003780"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDV2232419.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPTIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            4971..5906
                     /gene="ldtA"
                     /locus_tag="RHX88_003781"
     CDS             4971..5906
                     /gene="ldtA"
                     /locus_tag="RHX88_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV2232420.1"
                     /translation="MMRRVNIFCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5962..6037)
                     /locus_tag="RHX88_003782"
     tRNA            complement(5962..6037)
                     /locus_tag="RHX88_003782"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6002..6004),aa:Asn,seq:gtt)
     gene            6364..7281
                     /gene="nac"
                     /locus_tag="RHX88_003783"
     CDS             6364..7281
                     /gene="nac"
                     /locus_tag="RHX88_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDV2232421.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGEFN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            7383..8333
                     /gene="cbl"
                     /locus_tag="RHX88_003784"
     CDS             7383..8333
                     /gene="cbl"
                     /locus_tag="RHX88_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDV2232422.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKSFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(8371..8446)
                     /locus_tag="RHX88_003785"
     tRNA            complement(8371..8446)
                     /locus_tag="RHX88_003785"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8411..8413),aa:Asn,seq:gtt)
     gene            8451..>9196
                     /locus_tag="RHX88_003786"
     CDS             8451..>9196
                     /locus_tag="RHX88_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN/FAD transporter"
                     /protein_id="HDV2232423.1"
                     /translation="MLRHILTAKNLLSNPIFNFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALLQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLI"
BASE COUNT         2325 a         1980 c         2374 g         2517 t
ORIGIN      
        1 atcgcataat cactaaagtg atctgtacct acttttgtta tgaaataaac atcagtatcg
       61 gcaaagcttg ctgccagcgc ctggttacag ccttttcccc ccggtgaaaa tataaaatta
      121 cttgccagaa gcgattctcc gatagtggga agcctcggca aataactgat gatatcaaca
      181 ttaaacgaac caataacgca tacttgactt ttcatagttg agtggccttt ttttattccc
      241 tgggctgtat cataattttc aagcgcataa gcgatttcat ttttcgcaat agtatcaagc
      301 gagtctagga cgataaaagc accgccatga catcgagtca gatagccaag cctggcaagt
      361 tcattaagat ctgaacgaat agtttctttc gttacattga ggctattgga cagatcggat
      421 acttttacag aaccgaattt actgagtcgt ttcaatattt catgatggcg ttcaaatttc
      481 atgcttcacc tcacttcagt tacgttaata tctatcgatg tatattgatg tgataatcac
      541 cataaagtgg cggggaaaag caaaaaaaag caaaaatagc ctgagaaaag gtaagcgtta
      601 atggagaatc atattgatgc ttatttattg gtgagaacta cgttccatgg caggagttca
      661 tcaatacggt tggaaggtta ttccggcagt acgctcagga tatggcgcag atacgcttcc
      721 ggatcaatgt cgttcagaag gcaagtgccg ataagcccat acaacagtgc actgcgctcg
      781 ccgccgaaga acataaagtt tttatttccg agacagatgg cacaaagggc tttttccgcg
      841 gcattatgtc tgcctccgcc tgaccgtcaa cactgtaata acagagtgcg ccccactgat
      901 tcagtacaca gctgaaccat tctcccagtc tggatttttg gatagcgtgg catttttctc
      961 caccatgcat gcagtgagtc cagtaacagt ttgctcttct tctgcctgac cacaagatgt
     1021 gctggactgg taaacgatgg ggatacctgc ttacgcgaga gcaataagtg gctggccaca
     1081 attctcggta caaaactctg ctgaatagca gcgattttcc cagcgtggtg cgtcgtttgc
     1141 attttactgg ttctaagtta acaattatct gttcactaat gactactcta aaaacttgta
     1201 tcaggactat cacttattta agtgatattg gttgtctgga gattcagggg gccagtctat
     1261 aaacttttta caaaaggatg aatgcagaag gttactctaa tggccagagt gatgacaatg
     1321 atggtcagac gttgacctgc agaaactgca ttttaattgg tctataaagt ctgcaagagg
     1381 aatcagggtc agatcagtga gtcttatcgc ggcccgaagc tatgactgaa agaaggctgg
     1441 atacagaatt gcaacaattt ggcaatgtca gaacacagaa gctttgcaag caggtgaaag
     1501 gttatgtcta gcaaaaggag tatatcagaa tacatctact gatgtacaaa acacaacaat
     1561 agcttcatga tatataaata cgtgatgacg agtggtactc cgcatggtta tttaacttct
     1621 cagcaccact aatgatcctg agcaacttcg tatttgggcc attgccatgg agtagaaagc
     1681 tgtgtctgag aatgcagaat ttgagaaaag aattcgcttt cttggagagc ttctgaaaat
     1741 ggcacgctaa caacgttttg ttaaaaaatg tgaatcactt gccggggctg cattttctat
     1801 tcgaggagaa tgttgacgta gatattacag aaatctcagc gcatcaggat aaactgctac
     1861 cttaggtcaa ccaccaaaag aaaatacaat acgtccagtt tgtatgcctt taataacaca
     1921 tcttttaaat gttgaagaat tatcccgcct gctgaagaac gttgctctga gtgtgataaa
     1981 cctttttatt cttgatgatg tcagcgagaa gatggaatat atccccgact gggtcgtggt
     2041 tacccggtta aacatctggt ggcgctaata aatctggcaa gtcacattct gttgaatgct
     2101 gtgacagtgc cttaccgaga gagcgaaatt gtggtgttct agtgcatagt cgccaccatt
     2161 ccggataatt ctattgcgtt gttttacaag ttcttttaca gcgcagatct gtcgctgacg
     2221 ttgaatcaat agcctggatt tcagaacaca atgcccgttt gctggttggt ccggcagtag
     2281 cattaattca ttatctgaat gaaatgtgtc aatccacaga aaccgctgtc aatgacacat
     2341 tttgtcggac gttaacacga gaaagtctgg ctattccgta atgccatctt atttcgtaat
     2401 ggattgagaa tgctggattt ttaattatat tacgcgcagt attaatcgcg taaatgtttt
     2461 gcggtcagca acggtggggg atccattgtt ctgttcaatg aataccatca tacatttgaa
     2521 tcaggagtcg gtatgatgat tttggtgacg gggggcgcac ggagcgggaa gagtcgccac
     2581 gcagaggcgc ttattgggga ctcttcacag gttctgtata tcgctacctc gcaaatcctt
     2641 gatgatgaga tggctgcacg gatagaacat catcggcaag gccgcccgga gcactggcgc
     2701 acagtggagc gctggcaaca tcttgatgaa ttaattcatg cagacattaa cccgaatgag
     2761 gttgtgttgc tggaatgcgt taccacaatg gtgactaatc tgttgtttga ttatggcggc
     2821 gataaagacc ctgatgaatg ggattatcag gcgatggaac aggcgattaa tgctgagatt
     2881 cagtcgttga ttgctgcctg ccaacgttgc cccgcaaagg ttgtattagt gactaacgaa
     2941 gtgggaatgg ggattgtgcc ggagagtcgt ctggcacgac attttcgtga tattgccggg
     3001 cgggtaaatc agcagttggc cgctgcggca aatgaagtat ggctggtggt ttcgggtatt
     3061 ggagtaaaaa tcaaatgagt aaattatttt gggcgatgct ctcatttatt acgcgtctgc
     3121 ctgtaccgcg tcgctggtcc cagggactgg atttcgagca ttattctcgc ggtattatta
     3181 cttttccttt aattggatta ttgcttggcg cgattagcgg gctggtcttc atggtgctgc
     3241 aggcatggtg tggcgtacca ctggcggcac tgtttagcgt acttgtgctg gcgctgatga
     3301 ccgggggatt ccacctggac ggcctcgccg atacctgtga tggcgtattt tctgcacgta
     3361 gccgcgatcg catgttggaa atcatgcgtg atagccgttt aggcacccat ggcggtctgg
     3421 cattgatttt tgttgtactg gcaaagattc tggtattaag cgagttggct ctgcgtggcg
     3481 aaccgatcct tgcatcgctg gcggcagcat gtgcggtcag ccgcgggact gccgcattat
     3541 tgatgtatcg tcatcgttat gcgcgggaag aagggcttgg caatgtgttc attggcaaaa
     3601 ttgatgggcg acaaacctgc gtcacactcg gcttagccgc tatttttgct gccgtattat
     3661 tgccgggtat gcatggtgtg gctgctatgg tggtgacgat ggtggcgatt ttcatcctcg
     3721 gtcagcttct caaacgtacg ctgggcgggc aaaccggcga tacgctggga gctgctatag
     3781 agcttggtga actggtcttc ctgctggcac tgctataaag agacgtgaca tgcaaacact
     3841 tgccgattta ctgaatacga tccctgctat cgatcctgcc gctatgtcgc gtgcacaacg
     3901 gcatattgac gggttactca aacctgttgg tagcctggga aggctggagg cgcttgccat
     3961 acaactggcg ggaatgccgg ggttgaatgg cataccgcat gtgggcaaaa aagcggtact
     4021 ggttatgtgt gccgatcacg gcgtctggga ggaaggggtc gctatttccc caaaagaagt
     4081 gacagccatt caggctgaaa atatgacccg cggaacaacc ggcgtgtgtg tgctggcagc
     4141 acaagcgggc gctaacgtcc acgtagttga tgttggtatt gatagtgctg agcctatccc
     4201 cgggcttatc aacatgcgtg tcgcacgagg tagcggcaat attgcttcag ctccggcaat
     4261 gagtcgccgt caggctgaaa agttgctttt ggacgtcata tgttatacgc gggagctggc
     4321 aaaaaacggt gtcacgctgt ttggtgtagg tgaactgggg atggcaaaca cgacaccggc
     4381 agcggcaata gtcagcacaa tcactggccg ggatcctgaa gaagtggttg ggattggcgc
     4441 aaacctgccg acagataaac tggctaataa aattgatgtt gtgcgccggg cgattacgtt
     4501 gaatcaacca aatccgcagg atggcgttga tgtcctggca aaagtgggtg gatttgattt
     4561 ggtcggaatg gctggcgtga tgttaggcgc tgcttcctgc ggtttacccg tgttgctgga
     4621 tggatttctt tcttatgctg ccgcgctcgc agcctgccag atgtctccta caatcaaacc
     4681 gtatctcatt ccttctcacc tgtcggcaga aaaaggggcg cgtattgcgc tgtcgcattt
     4741 ggggctggag ccttatctca atatggagat gcgtttaggt gaggggagtg gcgcagctct
     4801 ggcgatgccc atcatcgaag ctgcctgtgc gatatataac aacatgggcg aacttgctgc
     4861 cagtaatatt gttctaccgg ggaatacgac ttctgatttg aacagttaaa aacgaaaagt
     4921 ctgataaaag ctatacttaa cggatagctt tcgcgactta ggaaagggac atgatgcgtc
     4981 gtgtaaatat tttttgctca tttgctctgc tttttgccag ccaaaatagc ctggcggtaa
     5041 cttatccatt acctccagag ggtagccgtt tagtggggca gtcgcttact gtaactgttc
     5101 ctgatcacaa tactcagccg ctggagactt ttgccgcaca atacgggcaa gggttaagta
     5161 atatgctgga agcgaatccg ggcgctgatg tttttttgcc gaagcctggc tcacaactca
     5221 ccattccgca gcaactgatt ttgcccgcca ctgttcgtaa agggattgtg gttaacgtcg
     5281 ctgaaatgcg tctttattac tatccgccag acagtaatac agtggaagtc tttcctattg
     5341 gtatcggcca ggctgggcga gaaactccgc gtaactgggt gactaccgtt gaacgtaaac
     5401 aagaagcacc aacctggacg ccaacgccga acacccggcg cgaatatgcg aaacgagggg
     5461 agagtttgcc cgcatttgtt cctgcggggc ccgataaccc catggggctg tacgcgatat
     5521 atattggcag gttgtatgcc atccacggta ccaatgccaa ttttggtatt gggctccgtg
     5581 taagtcaggg ctgtattcgt ctgcgtaatg acgatatcaa atatctgttt gataatgttc
     5641 ctgtaggcac tcgtgtacaa attattgatc agccagtgaa atacacaacg gaaccggatg
     5701 gttcaaagtg gctggaagtt catgagccgc tgtcgcgcaa tcgtgcagaa tatgagtctg
     5761 accgaaaagt gccattgccg gtaaccccat ctttgcgggc gtttatcaac gggcaagaag
     5821 ttgatgtaaa tcgcgcaaat gctgcgttgc aacgtcgatc gggaatgcct gtgcaaatta
     5881 gttctggttc aagacagatg ttttaagagc gttgataatt agaaagtaaa aagcctgctc
     5941 gaaagcaggc ttttttgaat ttggctcctc tgactggact cgaaccagtg acatacggat
     6001 taacagtccg ccgttctacc gactgaacta cagaggaatc gtgtgaacgg ggcgcatatt
     6061 aatggcagtg catggtgatg tcaaagggtg aaatgcattt ttttgtttgt ttgctgccaa
     6121 tttcaacaaa gtggtgagct ttcaatctta ttgggtaatg aaccatcgtg gtgcataccc
     6181 tccttttata gggcaggtga atgcgactgc agataaaaag agccaatgac gaatctatca
     6241 taatctctta tttgacagtt ggttagcttg cgcatcaacg ccaaaataga actggcaagc
     6301 atcttgcatt ctggttgtaa gtaatggcgg cacttgggcc gactcttaaa aaccggaggc
     6361 aacatgaact tcagacgcct gaaatacttc gtaaaaattg tagatattgg tagcctgacc
     6421 caggctgctg aagtattgca tatcgcacaa ccagcgctca gccagcaggt tgccacactg
     6481 gaaggtgagt ttaatcaaca acttttgatt cgcacaaaac ggggcgttac gccaactgac
     6541 gccggaaaaa ttctctatac ccatgcgcgg gccattttac gtcagtgtga gcaggcccaa
     6601 ctggcggtgc ataacgttgg tcaggcatta tcggggcaag tctcgattgg ctttgcgcca
     6661 ggaaccgctg cgtcatccat caccatgccc ttattacagg cggttcgcgc tgaattcccg
     6721 gaggtcgtta tctatcttca tgaaaatagc ggtgcagtgc ttaacgaaaa actgataaat
     6781 caccaactcg atatggcggt gatttatgag cattcccctg tggctggtgt atccagtcag
     6841 gctttactga aagaagatct tttcctggta gggactcaag attgtccggg gcaaagcgtt
     6901 gatgttaatg ctattgcgca aatgaacctc tttcttccca gtgattacag tgctgttaga
     6961 cttcgtgttg atgaggcttt ttccctacgg cgactcacgg caaaagtcat tggtgaaatt
     7021 gagtctattg ccacgcttac cgcagcgatt gccagcggca tgggcgttgc agtattaccc
     7081 gaatcggccg cgcgttcgtt atgtggcgca gtaaacgggt ggatgtcacg cattaccaca
     7141 ccttccatga gtctctcttt gtcattaaat ttacccgcca gagcgaactt atcaccacag
     7201 gcacaggcag tgaaagagtt gttaatgtca gtgatgagtt ctccagtgat ggaaaaaagg
     7261 cagtggcaat tggtgagcta agcgttatat cgggatggaa taagatgcgg gtttttatta
     7321 tttgttatgc cgggcattag actttaacaa taacggaaaa tctgaactgc ccggagttta
     7381 ccgtgaattt ccaacaacta aagataatcc gcgaggctgc acgtcaggat tacaacctga
     7441 cagaggttgc gaatatgctt tttacctcac agtccggcgt cagccgtcat attcgggaac
     7501 tggaggatga acttggcatc gaaatatttg ttcgacgagg taagcgactg ctgggcatga
     7561 ctgaaccggg caaagcatta ctggtcattg cagaacgtat tctgaatgaa gccagtaatg
     7621 ttcgtcggct tgcagacctg tttaccaacg atacgtctgg cgttctcact attgcaacga
     7681 cgcatactca ggcacgttat agtttgccag aggtcattaa atcttttcgc gaacttttcc
     7741 cggaggttcg gctcgagcta atccagggga cgccacagga aattgcgacg ttgttgcata
     7801 atggtgaagc tgatattggt atcgccagcg agcgtttgag taatgacccg cagctcgtcg
     7861 ccttcccgtg gtttcgttgg caccatagtt tgcttgttcc actcgatcat cccttgacgc
     7921 aaattacacc attgacgctg gaatcaatag cgaagtggcc gttaatcact taccgacagg
     7981 ggattacggg gcgctcacgt attgatgacg catttgcccg caaaggtttg ctggcagata
     8041 ttgtattaag tgcgcaggat tctgatgtca ttaaaaccta tgttgctctt gggcttggga
     8101 tcggaatagt tgccgaacaa tccagcggtg aacaagagga aaagaattta atccgcctgg
     8161 atacgcggca tctttttgat gctaatactg tctggttggg actgaagcga ggacaacttc
     8221 agcgtaacta tgtctggcgc tttctggaac tttgtaatgc agggctttca gttgaggata
     8281 tcaagcggca ggtgatggaa aacagtgaag aggaaattga ttatcagata tagcaaaaag
     8341 cccgctataa aagcgggctt ttcagaaatt tggctcctct gactggactc gaaccagtga
     8401 catacggatt aacagtccgc cgttctaccg actgaactac agaggaatcg ctgttgaggc
     8461 acatcttaac ggcgaaaaat cttttgtcaa acccgatttt taatttcccc aactgtttgc
     8521 cgtttctatc aacagtttgt tgcatttgca gacaatttgt tggcgaaaat ctttgcagct
     8581 ttgctgattc tccctcatta tttgaaatgt ggtttcactt tctgcaatta aggtcggctt
     8641 tgaatatctc ctctgcttta ctccaggttg ttcacggcac tcgctggcac gctaaacgca
     8701 agagctacaa agtgttgttc tggcgcgaga taaccccgct tgctgttcct atcttcatgg
     8761 agaatgcctg tgtcctgttg atgggggttc tgagcacttt tctggtcagc tggctgggaa
     8821 aagatgcgat ggccggcgtg ggattggcgg acagcttcaa tatggtcatt atggcttttt
     8881 ttgctgctat cgatcttggt actactgtcg ttgtggcatt tagtctcggt aagcgggatc
     8941 gacgacgagc gagggtggcg acgcggcagt cattggtgat catgacgttg tttgccgtac
     9001 tgttggcaac gcttattcat cattttggcg agcaaattat tgatttcgtc gcgggtgatg
     9061 ccacgacaga agttaaagca ctggcgttga cttatctgga gctgacggta ctcagttatc
     9121 cagcagctgc catcactttg attggtagcg gggcacttcg tggtgcaggg aatacgaaaa
     9181 taccgctact gattaa
//
LOCUS       DAPGWD010000167         9034 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPGWD010000167 DAPGWD010000000
VERSION     DAPGWD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.168"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            151..5571
                     /locus_tag="RHX88_003787"
     CDS             151..5571
                     /locus_tag="RHX88_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HDV2232424.1"
                     /translation="MNRVYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGKGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTVHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPPDTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYIDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEE
                     TLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5794..6060
                     /gene="hipB"
                     /locus_tag="RHX88_003788"
     CDS             5794..6060
                     /gene="hipB"
                     /locus_tag="RHX88_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDV2232425.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6060..7382
                     /gene="hipA"
                     /locus_tag="RHX88_003789"
     CDS             6060..7382
                     /gene="hipA"
                     /locus_tag="RHX88_003789"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDV2232426.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     SLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7694..7902
                     /locus_tag="RHX88_003790"
                     /pseudo
     CDS             7694..7902
                     /locus_tag="RHX88_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8334..8897
                     /gene="fimA"
                     /locus_tag="RHX88_003791"
     CDS             8334..8897
                     /gene="fimA"
                     /locus_tag="RHX88_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV2232427.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT         2467 a         1916 c         2418 g         2233 t
ORIGIN      
        1 ttcgaaagta ttagcaaatt agccaaattg acaccttccc acctccaact aacactcaat
       61 acagacctct gacaatttaa taagtttcat ttttaatgtc tgacgactca tctctttcgt
      121 ggctgtattg tttgtatatg gagcgtttat atgaatagag tctatcgcgt gatatggaat
      181 tgcactctac aggtatttca ggcctgctcg gaattaactc gcagggcagg taaaacatcg
      241 acggttaatt tgcgtaaatc ctctggactg acaacgaaat tcagtagatt gacgctgggt
      301 gttttgctgg cactaagcgg ttcagcgtct ggtgcaagtc tggaagttga taatgatcag
      361 attaccaata ttgatactga tgttgcttat gatgcctacc tggttggctg gtatggcact
      421 ggagtgctta atattttggc tggcggtaat gcctccttaa ccactattac taccagcgtc
      481 attggcgcta atgaggactc agagggcacc gttaatgttt tgggtggcac ctggcgattg
      541 tatgatagcg gaaataatgc aaggccttta aatgtgggtc aatccggaac ggggacgctg
      601 aatattaaac agaaaggtca cgttgatgga ggctatttaa gactaggttc ctcgacagga
      661 ggcgtcggga cggtcaatgt tgagggagag gactctgttc tgacgaccga attattcgaa
      721 atagggagtt acggtacggg ctcattaaat attacggata agggttacgt cacgagttca
      781 atagtcgcca ttttaggcta tcaggcgggc agtaatggcc aggttgtcgt tgaaaagggc
      841 ggtgagtggc taataaaaaa taacgattcc tcaattgaat ttcaaattgg taatcaagga
      901 actggggaag cgactattcg cgagggtgga ctgattacgg ctgaaaacac gattatcggt
      961 ggcaatgcta ccggtatcgg aaccctgaat gtgcaggatc aagactctgt catcacggta
     1021 cgcagactct acaatggata tttcggtaat ggcacactca atatttccaa taatggacta
     1081 attaataaca aagaatattc attagtgggc gttcaggacg gttcccacgg tgtcgtcaac
     1141 gtgaccgata aagggcattg gaatttcctc ggaacgggcg aagctttccg ctatatctat
     1201 atcggtgatg ctggcgacgg tgaacttaat gtctcgcgtg aaggtaaagt agattcggga
     1261 attatcactg cggggatgaa agaaacaggc acaggcaaca ttactgttaa ggataagaac
     1321 tccgttatca ctaatctcgg aactaatctt ggttatgacg gccacggcga aatgaatatc
     1381 agtaatgaag ggcttgttgt cagcaacgga ggaagctcac tcggttatgg agaaaccggc
     1441 gtcgggaatg tcagcatcac cacggggggg atgtgggagg tcaataagaa tgtctatacc
     1501 accattggtg ttgcgggcgt cggaaacctc aatattagcg atggcggtaa gttcgtatcg
     1561 caaaatatta cttttttggg cgataaagca agcggtatcg gcacactgaa cctgatggat
     1621 gcgacatcat cgttcgatac tgtgggtatc aatgtcggta attttggtag cggtatcgta
     1681 aatgtcagta atggtgccac ccttaattca acgggctatg gatttatcgg aggaaatgcc
     1741 tccggtaagg ggatagttaa tatttcaacg gacagtctct ggaatttaaa gacatcatct
     1801 actaacgcac aattgctaca ggtcggtgta ttaggcaagg gtgaactgaa tattaccacc
     1861 ggaggtatag ttaaagcgcg tgatacacag atagctctca atgacaaaag taagggcgac
     1921 gtgagggtgg atgggcagaa ctctcttctt gaaacattca atatgtacgt agggacatct
     1981 ggtacgggta cgttaaccct gacgaatagc ggcacgctga atgtcgaagg tggagaagtt
     2041 tacttaggtg tttttgaacc tgctgtagga acgctaaaca ttggtgcagc tcacggtgag
     2101 gcggcggcag atgccgggta tataaccaac gcgacgaaag tagagtttgg ttctggcgaa
     2161 ggtgtttttg tctttaatca tactaataac agtgatgccg gctaccaggt cgatatgctg
     2221 attacgggtg acgataaaga cggaaaagtg atccatgatg caggccatac ggtgttcaat
     2281 gcagggaata cttatagcgg taaaacgctg gtcaatgacg gcctcctgac cattgcgtct
     2341 catacggcag atggggtaac gggcatgggt tcgagtgaag tcaccattgc aagccccggt
     2401 acgctcgaca ttctcgcatc aacgaacagt gcaggagatt acacgctgac caatgcgctc
     2461 aaaggcgatg gtttgatgcg agtgcagctg tcatcctccg acaagatgtt tggctttacc
     2521 catgcaacag ggactgaatt cgccggtgtt gcccaactga aagacagtac cttcactctg
     2581 gagcgcgaca acaccgctgc gcttactcac gcgatgttgc agtctgacag tgaaaatacc
     2641 acatcggtaa aagtgggaga gcaatccatt ggtggactgg ccatgaatgg tggtacgctc
     2701 attttcgata cggatattcc tgctgcgacg cttgcagagg gatatatcag cgtcgatacg
     2761 ctggttgtcg gcgcgggtga ctacacctgg aaaggccgta actatcaggt aaacgggacg
     2821 ggcgacgtgc ttatcgacgt gcctaaaccg tggaatgatc ccatggcgaa taaccctctg
     2881 acgacgctca atttgctgga acacgacgat agccatgtcg gcgttcaact ggtgaaggcg
     2941 caaacggtta ttgggtcggg tggctcatta acgttacgtg atttacaggg cgacgaggtg
     3001 gaagcggaca aaacggtaca cattgcgcaa aacggaacgg tggtcgccga gggtgattat
     3061 ggattccgcc tcacgaccgc accaggtgat ggtttgtacg ttaactatgg gctgaaagcg
     3121 ctgaacatcc atggtgggca aaagctgacg ttagccgaac atggcggagc ctatggcgca
     3181 acggccgata tgtcggcaaa aatcggtggt gaaggggatc tggcaatcaa tacggtgcga
     3241 caggtttcgc tttccaacgg tcagaacgac tatcaggggg caacctacgt tcagatgggg
     3301 acattacgta ccgatgcgga tggtgcactt ggcaacaccc gggaactgaa catcagcaac
     3361 gcggccatcg tcgatcttaa tggatcgacg cagacggtag agacattcac cgggcagatg
     3421 ggttcgactg ttttgttcaa agagggggcg ctgacggtaa ataaaggtgg gatcagtcag
     3481 ggtgaactga caggtggcgg aaacctgaat gttacagggg gaacgctggc tatcgagggg
     3541 cttaatgcac gctacaatgc gttaaccagc attagcccaa atgcggaagt cagcctcgat
     3601 aatactcagg ggttaggcag aggaaatatt gccaatgacg gtctgttaac gctaaaaaac
     3661 gtgactggcg aactgcgtaa tagcataagc gggaagggta tcgtgagcgc aaccgccagg
     3721 acagatgtag agctggatgg cgataatagc cgctttgtgg ggcaattcaa cattgataca
     3781 ggcagcgcgc tcagcgtcaa cgagcagaaa aacctgggtg atgcttccgt tatcaataat
     3841 ggcctgctca ccatctccac tgagcgtagc tgggcgatga cgcacagtat cagcggtagc
     3901 ggtgatgtga caaaactggg taccgggatc ctgactctta acaacgattc cgcggcgtat
     3961 cagggtacga cggatatcgt ggggggggaa attgctttcg gttccgactc tgccattaat
     4021 atggcaagtc aacacattaa tatccataac agcggtgtga tgtcgggaaa tgtcaccact
     4081 gcaggtgatg tgaacgttat gcctgggggg acactgcgtg tcgctaaaac cacaatcggc
     4141 ggcaacctgg agaatggcgg cacggttcaa atgaatagcg aaggggggaa accggggaat
     4201 gtactgaccg ttaacggcaa ctataccgga aacaatggcc tgatgacgtt caacgcgacg
     4261 ctgggcggcg ataattcgcc caccgataaa atgaacgtga aaggcgatac ccaagggaac
     4321 actcgcgttc gggttgataa cattggcggc gtcggtgcac aaacggtcaa cggtattgaa
     4381 ctcattgagg ttggcggtaa ttctgcaggt aacttcgcgc tgaccaccgg aactgtcgaa
     4441 gctggggctt acgtctacac gctggctaaa gggaagggga atgacgagaa aaactggtat
     4501 ctgaccagta aatgggacgg cgtaacgcca ccggatacgc ccgaccccat caataatccc
     4561 cctgttgtgg atccggaagg cccatcagtt tatcgcccgg aggccggaag ctatatcagc
     4621 aacattgccg cagccaactc gctgtttagc catcgtttac acgaccgtct gggtgagccg
     4681 cagtatatag attcactgca ttctcagggg tcggcaagca gtatgtggat gcgtcatgtc
     4741 ggagggcacg aacgttcaag ggccggtgac ggtcagctaa atactcaggc taaccgctat
     4801 gtattgcagc taggcggcga tttggcgcag tggagtagca acgcgcagga tcgctggcat
     4861 cttggcgtga tggcaggtta cgctaatcag cacagtaata ctcagagtaa tcgtgtgggt
     4921 tataaatcgg atgggcgcat cagcggttac agcgctgggc tgtacgcgac ctggtatcag
     4981 aacgatgcga ataagaccgg cgcttatgtt gacagctggg cgctgtataa ctggtttgat
     5041 aacagcgtca gttccgataa ccgttctgct gacgactatg attctcgcgg tgtgacggcc
     5101 tctgttgagg gtgggtatac ctttgaagcg ggaacattta gcggcagcga agagacgctg
     5161 aatacctggt acgtccagcc acaggcgcaa atcacctgga tgggtgtgaa agattccgac
     5221 catacccgga aagacggaac gcgcattgaa acggaaggcg acggaaatgt gcaaacgcga
     5281 cttggggtga aaacctacct gaacagccat caccagcgtg acgatggtaa acagcgtgag
     5341 ttccagcctt acattgaagc gaattggatc aacaacagca aagtctacgc cgtgaagatg
     5401 aatggtcaaa ccgtaggccg tgaaggtgcg cgtaatctcg gtgaagtacg taccggggtt
     5461 gaggcgaaag taaataacaa ccttagcctg tgggggaatg tcggtgtgca actaggtgat
     5521 aaaggctata gcgatactca gggcatgctg ggagtgaaat atagctggta aaccgtataa
     5581 gccgcatgtc gagatggcat gcggcttaat attgccgact tcaaacggcg catcagcgcc
     5641 ttttttaaat cctccttttt atccgcgatc gcggatatcg cagcgtttat cccgtagagc
     5701 ggataagacg tgtttccaaa ttgacttacc ctcactaaag gataaaactg ataatatcct
     5761 cttatgcgga taaatttgct gtggacgtat gacatgatga gctttcagaa gatctatagc
     5821 ccaacgcaat tggcgaatgc aatgaaactg gttcgccagc aaaatggctg gacgcagagc
     5881 gagctggcga aaaaaattgg cattaagcag gcgacaattt ccaatttcga aaacaaccct
     5941 gacaatacca cgctcacgac attttttaag attttacagt cgcttgaact ctcaatgaca
     6001 ctatgcgaca cgaaaaatgc ctcgccagaa tcaacagaac agcaagatct ggagtggtaa
     6061 tgcctaaact tgtcacttgg atgaacaacc agcgggtagg cgagttaacg aagttagcca
     6121 acggcgcgca cacctttaag tatgcaccgg agtggttagc cagccgttat gccagaccgt
     6181 tgtcactttc gctgccattg cagaggggga atatcacctc agatgccgta tttaacttct
     6241 tcgataacct gttacccgat agcccgattg tacgtgaccg gatcgttaaa cgttatcatg
     6301 ctaaatccag acaaccgttt gatttattgt cagaaatagg gcgagacagc gttggtgccg
     6361 taacgtcatt acccgaaaat gaaaccataa cgcgcccgat aatggcatgg gaaaagctta
     6421 ctgaagccag gcttgaagaa gtattaacgg cttataaagc agatatccct ctgggcatga
     6481 ttagagaaga aaatgacttt cgcatctcgg ttgctggcgc gcaggagaag acagcgctgc
     6541 ttagaatagg caatgactgg tgcattccga aaggaataac gccgacgacg cacatcatta
     6601 aattaccgat tggcgaaatc aggcagccca atgcgacgct cgatctcagc caaagcgttg
     6661 ataatgagta ttactgtctg ctgctggcga aagaacttgg gttgaatgtt ccggacgcag
     6721 aaatcattaa agcgggaaat gtgcgcgcgt tagcggtcga acgttttgac aggcgttgga
     6781 atgctgagcg aacggtttta cttcgcttgc cacaggagga tatgtgtcag acattcggtt
     6841 taccttcatc tgtgaaatat gaatcagatg gaggcccagg catcgcgcgg atcatggcgt
     6901 ttttgatggg gtccagcgag gcgcttcgcg atcgttatga ttttatgaaa ttccaggtct
     6961 tccagtggtt gattggcgca acggatggac atgcaaaaaa cttctccgta tttattcagg
     7021 ctggcggcag ttatcgactc acgccatttt acgacatcat ttcagcattt ccggtccttg
     7081 gcggtacggg aatacacatc agcgatctca aactggcaat ggggcttaac gcatccaaag
     7141 gcaaaaaaac ggcaatcgat aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc
     7201 tgagattccc ggaagtgcag atgcatgaaa tcctaagtga ctttgccaga atgattccgg
     7261 cagcactgga taacgtgaag acttcattac cgacagattt tccggagaac gtggtgacgg
     7321 cagttgaaac caatgtgttg aggttgcatg gacggttaag ccgagaatac ggtagtaagt
     7381 gagatatggg gtttggtcgt tgttaatgag cacgacaatc atgaccgcca acgactggat
     7441 tcttccttgg tttatacttg tgcgcataat gtatattatg ttaaatcatg tctgagtgat
     7501 gcgaagtgct tttctggtag tcgttattcg ttcagggttc aagaacttaa gcctctcctc
     7561 tttcataatt ggcctgtgca tggagaaata gcctggttat tccctgcccc atgctaagag
     7621 cgcagtgtct gtcgcataga cactgcatac gaaaacacca gagggtgctg ttacttattt
     7681 aaggacggat caagcgggaa tgagtccact ccggtatcag aaatggctac gtctcaatga
     7741 agtcaggcga ccgatgctga atgaacatta cgatgtcacc acagcagcct atgctgtcgg
     7801 ttacgaaagc tatcccattt cagtcgggaa tattcgcgga tgtttggaga gtcacccaag
     7861 agagatatta ccaggttaag aaagtctgtc ggtcagcttt aataaaccct gcacttatct
     7921 gtttagagca attcggtgtt agtttcagca agcaaacatt aaccatagct aatgatttat
     7981 agccatatta accattggta gcccttgacc tttcctgaca cttcccttgt tcgcaataat
     8041 attctgttta gtcacggttt ataaataaca tgcataatga aatcgtcatg ttatttagcg
     8101 acataaaatg tttttgcaaa tactctcttc aacaagtgca caataaactt gttgtgatta
     8161 ggtgtccttg tgatttaaag atattatcgt tcttcgtgaa atgtaataac cctggaaact
     8221 atattctcag ggcaacaacc aatcaagaat gattcttgtt tttaagcaat aacagaatga
     8281 cttaattgtt aaccctcttt attcagctac ttaaatataa attttggaga attatgaaac
     8341 tcaaacatgt tggtatgatt gtcgtttctg tgttggcgat gtcgtctgct gcggtaagcg
     8401 cagccgaggg tgatgaatca gtaacgacca ctgttaatgg cggtgttatt cattttaaag
     8461 gtgaagtggt aaatgccgct tgtgcaattg attcagaatc aatgaaccaa acggttgagc
     8521 tgggtcaggt tcgttcttct cgcctggcta aagcgggtga cctcagctcc gccgttggct
     8581 tcaatatcaa gctgaatgat tgtgatacca atgtttccag taatgcagct gttgcattcc
     8641 tgggtactac tgtcaccagt aatgacgata cgttagcgct gcagagttca gcggcaggct
     8701 ctgcccaaaa tgtcggtatt caaattttgg accgtacggg tgaggtatta atacttgatg
     8761 gggccacttt tagtgctaaa accgacttga ttgatggcac gaatatactg ccattccagg
     8821 ctcgttatat tgctctcggg cagtccgtag ctggtactgc aaacgctgat gcgaccttca
     8881 aagttcaata tctataattc aatcttaagg tggctatacg tcatctgaaa ttgaaacagg
     8941 gtgtgtgtgg catggctacg cacacccgag caatttacca atattcatct aatttttaat
     9001 gagaaatggt ttgtatttgg agcgctatga aaaa
//
LOCUS       DAPGWD010000168         9007 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPGWD010000168 DAPGWD010000000
VERSION     DAPGWD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.169"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            66..338
                     /locus_tag="RHX88_003792"
     CDS             66..338
                     /locus_tag="RHX88_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDV2232428.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(339..1211)
                     /gene="rluF"
                     /locus_tag="RHX88_003793"
     CDS             complement(339..1211)
                     /gene="rluF"
                     /locus_tag="RHX88_003793"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDV2232429.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1423..2112
                     /gene="pepE"
                     /locus_tag="RHX88_003794"
     CDS             1423..2112
                     /gene="pepE"
                     /locus_tag="RHX88_003794"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDV2232430.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2203..3834)
                     /locus_tag="RHX88_003795"
     CDS             complement(2203..3834)
                     /locus_tag="RHX88_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDV2232431.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4054..7737)
                     /gene="metH"
                     /locus_tag="RHX88_003796"
     CDS             complement(4054..7737)
                     /gene="metH"
                     /locus_tag="RHX88_003796"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDV2232432.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEELAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            7937..8761
                     /gene="iclR"
                     /locus_tag="RHX88_003797"
     CDS             7937..8761
                     /gene="iclR"
                     /locus_tag="RHX88_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDV2232433.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2073 a         2477 c         2327 g         2130 t
ORIGIN      
        1 ttatcagttc agctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca
       61 ccatcatggc actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac
      121 ttaaaacact gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa
      181 ccaacagcat taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga
      241 aaaaagcccg ccagttgaag aaaaagcagg cttataaacc ggatcctgaa gcgtcattta
      301 gctggtcagc aaatacgtcg acgcgcggca ggcgctgatt agcgcccctt ctttttacgc
      361 cccggagagg taaaacgctt accgttggac gccgggcgac cgcctttttc cgccgttttt
      421 tccatcttaa cgactggacg tttgatgccc gctgttttcg gtttggcctt cactttaggt
      481 tttgcctcgg aagaggaatt ttcgatgagc ttaaagaggt cgattaactc atcatcagtt
      541 aaatcgcgcc actcacccag cggaatgccg cttaagctga cgttcataat gcgcgtacgt
      601 tccagctttt tcacttcata gccgaaatgc tcgcacatgc gccggatctg acggttcagc
      661 ccctgcacca gggtaatgcg gaagacaaac ggcgcttctt ttttgacttt gcactttttc
      721 gtcaccgtcc cgaggatcgg cacccccgcc cccataccac gaataaattc atcggtaatc
      781 ggtttatcga ccgtcaccag atactctttc tcatgatcgt tacccgcacg gaggatctta
      841 ttcaccagat cgccgtgatt ggtgaggaaa atcagcccct gggagtcttt atccaggcgg
      901 ccaatcggga acacgcgttt gctgtggtta acgaaatcga caatgttatc gcgctcgcca
      961 tcttcggtgg tgctgacaat accgacgggc ttgttcaggg cgataagtac caaatcttcg
     1021 gcttcccgag gctcaatcaa ctgaccgttt actttcacaa tgtcgccggg tttcacctga
     1081 tcgccaatgg tggctcgctt gccattaagg aacacattgc cttgctcgat atagcgatcc
     1141 gcttcgcggc gtgagcaaat tccgctttcg ctgatgtatt tatttaaacg gactgatgag
     1201 tcgggcagca taaattctcc tgtaaaaaac ggaatatacc gtacctttgg gttgataaaa
     1261 aatagtcatg agcgaactat ttgttatttc cattggcttg aattcgttac ctgctcaaat
     1321 cactttccag aagcaatgtg cgacatcgtg agccatgtca gtaagtggtt aaattcgcag
     1381 tggtagcatg ccttccgtga tttcttacta accggagaag taatggaact gcttttattg
     1441 agtaactcga cgctgccggg taaagcctgg ctggaacatg cactgccgct aattgctgaa
     1501 cagttgcagg ggcgccgctc agcggtgttt atccctttcg ctggcgtaac gcagacctgg
     1561 gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc tgggtgtttc tgtcaccggt
     1621 attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg ctgagatcgt gattgtcggg
     1681 ggcgggaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct ggcaccaatt
     1741 actgacgtgg ttaaacgtgg tgcgctgtat attggctgga gcgcaggcgc taaccttgct
     1801 tgcccaacca ttcgtaccac caacgatatg ccgattgtcg atccgcaagg tttcgatgcg
     1861 ctaaatctgt tcccgctgca aatcaacccg cacttcacca acgcgctgcc ggaaggccat
     1921 aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc agaactgacg
     1981 attattggtc taccggaagg taactggatc acagtgagta aaggtcacgc tacgctgggt
     2041 ggcccgaaca ccacttatgt gtttaaggct ggtgaagaag cggtgccgct agaagctggt
     2101 caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata gtgcctgatg
     2161 cgacgcttga cgcgtcttat caggcctaca ggattcacaa tattaatact cgtcccgtcc
     2221 ttcatcttca tccggctgtt ccagcacact gtaagccacc gaacaaaaca gcgagttcag
     2281 gcgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatgc tctgcacgtt
     2341 ttgctgatgc aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa tgcgaaaacg
     2401 atgtttgctg cgacgcaaac gacgagcgct ggtgacatcg ccagagaaga acaccgacat
     2461 tgccagtttt aaattactga gcaattgctc atagagcgca tccagttcct tcaagccatc
     2521 aagcgaaaat gcccgccgtg ctgccagcga tttatcggca atttcgctgc ccatgcgctc
     2581 gacgatatcg gaggcctgtt caaggttgag cgacatttcg atgatctccg cccagcggcg
     2641 cgactcttcc tccgccaact cttcttttgg catccgcgcc agatacagct taatggcggt
     2701 gtagagaacg ttgatatcat ctgccagctt acgcagctct ttctcctgcc gtggctcgcc
     2761 gtgcatcact ttatttagcc cttccatcat ctgttccatg gcgtcgccaa tgcgcagggt
     2821 ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga catccagatg
     2881 tttaggccgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt tgcaaaaccg
     2941 tgccatcggg tcaacaaatg gcagcatgac caggcaacgt acaaggttgt agaagacgtg
     3001 gaaatagatc accagttccg cttttggcaa tgacagcttc cccattgtct ctgccagcaa
     3061 atggacaaac ggcaggataa tcaggctacc caccagctta aacagcagac tacccagcgc
     3121 gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca ggccggaacc
     3181 gaggttagca ccaatcacca gacagagcgc cacggggaag gagataatgc ctgcggcggt
     3241 cagagtcgca gtcagcagta cagcagcaag gctggagtag ctgataatgg cgaacatcgc
     3301 gccaatcagc gcatccagca gaatatcgcc ggtcagcgag gcaaagatca cctgaacgcc
     3361 gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac tccagcgcca gtaaaatcag
     3421 cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc gtccgaggaa
     3481 aaaaatcacg ccgataaaaa taagtaacgg tgacagccag gataagtcga aggtgagaat
     3541 acgcgccatt agcgccgtcc cgacatctgc ccccagcaca atgaccagag ccggtgcgag
     3601 ggctaccaga tcctgagcga caaacgaggt caccagcatg gtggtggcat tactgctctg
     3661 caccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttttttt cgacgctccg
     3721 gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc gaacaatatg
     3781 agtcccccag accagcaggg cgacggcaga aagcaggtga agcagcgtta acacgagttc
     3841 aggttctcct tatcgttata tgttcctgaa gcaattgaat gaccaaagga ccggaaacac
     3901 gctgactggc atctttactg tgccttttga cattcgtttt tccggctaag attattccag
     3961 cctgtttcca gtataagtgt ttcatattaa taaagagaca gggcactcat tgagcgccct
     4021 gtttgttgta aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc ccagattcgg
     4081 tgccagccag cgctcaacat cggagacgct cataccttta cggcgggcat agtcttcaac
     4141 ctgatcgcgc tgaatttgcg ccaccgcgta gtacttgctg tccgggtggc tgaaatacca
     4201 acccgaaacc gatgcaccgg gccacatggc gaaagattct gtgagtttca tgccggtatg
     4261 tttttccact tccagcagct cccagatggt ggctttttcc gtatgttccg ggcaggccgg
     4321 gtagcccggt gccggacgga tgccctggta gttttcgcgg atcagttctt cgttgctgag
     4381 gttctcgttc ggcgcatagc cccagtagac tttacgcaca cgctcatgga gatactccgc
     4441 aaaggcttcg gctaaacggt cggcaagcgc tttcaccatg attttgttgt aatcatcgtg
     4501 ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca cggcaaatgc
     4561 gccgatgtaa tctgctttac cagaaagctt cggcgcaacg aagtcagcga gacagtagtt
     4621 agcgaatccc gttttttcgg tctgttgacg cagatggtgg ctgacgttga tcacatgggt
     4681 gcgcgtttcg tcacggtaga tttcaatgtc atcgcccaca cggtttgccg ggaacaggcc
     4741 caccacgcca cgcggattca gcgttttctc ggcgcttaat ttatccagca tgtcgttggc
     4801 gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa tgcgcggata
     4861 cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgtaat tacgcagagt
     4921 ttcgatgctg gcttcaactt cctgcacgcc gagacggtgc gccaccggcg gcgtgtaagc
     4981 ctgccagtca aaagcgaaat cgttatcgcg cgccgcttcc agcgtgaccg gtggtgtgcg
     5041 cggtttctta cgcccgtgct gaatacgtac ggtttcgtac tccttgcggg tacgagcgac
     5101 aaaatcatca cgctgagtat cggaaagcag cgcagccacc acaccaacgg tgcgcgatgc
     5161 gttctgtaca tacaccgtcg ggccgctgta gttctgctca attttcaccg ccgtgtgcgc
     5221 ttttgaggtc gtcgcgccgc caatcaatag cgggatggtg aagccctgac gctccatctc
     5281 tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa gtccaatcag
     5341 atcagcattc acttctttag cggtacggag aattttttcc gcaggcacca taacgccgag
     5401 atcgacaatt tcgtagttgt tacattgcag caccacacca acgatatttt taccgatgtc
     5461 gtggacgtcg cccttcacgg tggcgatcac catcttgccg ttggttttgc cctgctcttt
     5521 gctggcttca ataaacggtt cgagatacgc caccgcctgt ttcatgacgc gcgccgattt
     5581 gaccacctgt ggcaggaaca ttttcccttc gccaaacaga tcgccgacca cgttcatgcc
     5641 gtccatcaac gggccttcaa tcacttcaat cgggcgcgta gcctgctggc gggcttcttc
     5701 ggtatcctgc tcgataaact cggtaatgcc tttgaccagc gagtattcca gacgtttatt
     5761 cacttcccac gagcgccact ccgcctgctg ggcgttagcg gtgtcgtcgg ttttgctgcc
     5821 gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcatcgcgac gattaagaat
     5881 cacatcttcc accgcgtcgc gcagttcagc gggcaggtcg tcgtaaatcg ccagctgccc
     5941 ggcattgacg atccccatat ccatgccatt gcgaatagcg tagtagagga acactgcgtg
     6001 aatggcttcg cgcaccggat cgttgccacg gaacgagaaa gaaacgttag atacgccgcc
     6061 ggaaattagc gcgtgcggca gttcgcgttt gatgtcttca cacgcgccga taaagtcctg
     6121 cgcgtagttg ttgtgctctt caatgccggt ggcaaccgcg aagatgttcg ggtcgaagat
     6181 gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc gacggcaaat
     6241 ctcgattttc cgtgcgcggg tatcggcctg tccctgttcg tcaaaggcca ttaccaccac
     6301 tgccgcaccg taacgacgca agagcttcgc gtgatggata aaggcgtcga cgccctcttt
     6361 catcgagata gagttaacaa tgcctttgcc ctggatacac ttcagacctt tttcaatgac
     6421 atcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt caccggcaat
     6481 cagattgaga aaacgcacca tcgccgcttc ggcgtcgagc atcccttcat ccatgttgat
     6541 atcgataatc tgcgcaccgt tttccacctg ctggcgggcc acatccagtg cctcgctgta
     6601 tttctcttct ttgatcaggc gcttgaactt agcggaaccg gtgacgttgg tgcgttcacc
     6661 cacgttcaca aacaggctat cttcgccaat gttcagcggc tccaggccgg acaaacggca
     6721 ggctacgggg atttccggca gtttgcgcgg cgctaattct tctactgcac gactcatcgc
     6781 tgcaatatgt tgtggcgtgg tgccacagca gccgccgacg atattgagaa aacccgcttg
     6841 cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtact caccaaaggc
     6901 gttgggtagc ccggcgttcg ggtgcgcggt gacgtagcat tccgcaatcc gtgacagctc
     6961 ctgcacgtac tggcgcagtt catcgggccc cagcgcacag ttcaggccaa aggtcagagc
     7021 ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga gcgtgcgccc
     7081 ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca gcgcttcaaa
     7141 ctccgttttc accgcaaaaa ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat
     7201 caggatcaga tccgcaccac cttccaccag cgctttggtg gattcgcgat aggccgccac
     7261 cagctggtca aaagtgatgt tgcggaatgc cggatcgttg acgtccggag aaatagacgc
     7321 cgtgcggttg gttgggccga gaacaccggc aacgtagcgc ggtttctccg gcgtgcgcgc
     7381 ggtccactcg tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga tttccgccga
     7441 cagggattcc atctggtaat ccgccatcgc aatggtcgtg gagttaaagg tgttggtttc
     7501 gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca cttccggttt
     7561 gctgagcacc agcaggtcgt tgttgccttt gagatcgcat ggccagtcgg caaagcgttc
     7621 accacgaaaa tcggcttcgt tcagtcgata gctctggatc atggtgccca taccgccgtc
     7681 cagcaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc tgctcacact
     7741 tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac tggcataaac
     7801 gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt aactcatcgg
     7861 atcagttcag caactattgc attagctaac aataaaaatg aaaatgattt ccacgataca
     7921 gaaaaaggag gctgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt
     7981 tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga aattactgga
     8041 gtggattgcc gaatccaatg gcagtgtggc actcacagag ctggcgcaac aagccgggtt
     8101 acccaattcc acgacccacc gcctgctaac cacgatgcaa cagcagggtt tcgtgcgtca
     8161 ggtcggcgaa ctgggacatt gggcaatcgg cgcacatgcc tttatggtcg gcagcagctt
     8221 tctccagagc cgtaatttgt tagcgattgt tcaccctatc ctgcgcaatt taatggaaga
     8281 gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag cgattattat
     8341 cgaccaggta cagtgtacgc atctgatgcg aatgtccgcg cctatcggcg gtaaattgcc
     8401 gatgcacgct tccggtgcgg gtaaagcctt tttagcccaa ctgagcgaag aacaggtgac
     8461 gaagctgctg caccgcaaag ggttacatgc ctatacccac gcaacgctgg tgtctcctgt
     8521 gcatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg acgatgagga
     8581 acatgcactg gggctacgtt gccttgcagc gtgtattttc gatgagcacc gcgaaccgtt
     8641 tgccgcaatt tcaatttccg gaccgatttc acgtattacc gatgaccgcg tgaccgagtt
     8701 tggcgcgatg gtgattaaag cggcgaagga agtgacgctg gcgtacggtg gaatgcgctg
     8761 actttttctg gcgggcagag gcaatattct gcccatcata cctgagtggc aatagaataa
     8821 gggtgtctgt taatcgcatt gacgccaaaa taacttaatg tcatacactt cacacacttt
     8881 tattaactca gcattatttt taaacatcaa accacttaat cattaacaaa atcgcaagat
     8941 ctcattccac cgaaactttt acggctgcat tatgagattg agcgatttat gatatttctt
     9001 tagttgt
//
LOCUS       DAPGWD010000169         8917 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPGWD010000169 DAPGWD010000000
VERSION     DAPGWD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.170"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            102..1337
                     /gene="moeA"
                     /locus_tag="RHX88_003798"
     CDS             102..1337
                     /gene="moeA"
                     /locus_tag="RHX88_003798"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDV2232434.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            1337..2086
                     /gene="moeB"
                     /locus_tag="RHX88_003799"
     CDS             1337..2086
                     /gene="moeB"
                     /locus_tag="RHX88_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDV2232435.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARYALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(2265..2927)
                     /gene="fsa"
                     /locus_tag="RHX88_003800"
     CDS             complement(2265..2927)
                     /gene="fsa"
                     /locus_tag="RHX88_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV2232436.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            3058..3957
                     /locus_tag="RHX88_003801"
     CDS             3058..3957
                     /locus_tag="RHX88_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDV2232437.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3963..6395
                     /locus_tag="RHX88_003802"
     CDS             3963..6395
                     /locus_tag="RHX88_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDV2232438.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6541..7356
                     /gene="ybiV"
                     /locus_tag="RHX88_003803"
     CDS             6541..7356
                     /gene="ybiV"
                     /locus_tag="RHX88_003803"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDV2232439.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7508..8773
                     /locus_tag="RHX88_003804"
     CDS             7508..8773
                     /locus_tag="RHX88_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDV2232440.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         2116 a         2369 c         2385 g         2047 t
ORIGIN      
        1 gtaaaggcag gagccaacgc tacgcccgag gtggcataca gtctgtggcg tttttctgac
       61 ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt accaccggat
      121 tgatgtcgct cgacaccgcg cttaatgaga tgctttctcg cgtcacccca ctgaccgccc
      181 aggaaacgct gccactggta cagtgttttg gtcgtattct ggcgagcgat gtcgtttcgc
      241 cgcttgatgt tccggggttt gataactccg caatggacgg ctacgcggtg cgtttagccg
      301 atattgcctc caggcaaccg ctgcccgttg ccggtaaatc ctttgccggt cagccgtacc
      361 atggtgaatg gcctgcgggt acctgcattc gtattatgac cggtgcgccg gtgccggaag
      421 gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc gtgcgtttta
      481 ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc tctgcaggtg
      541 cggttgtttt cccggcggga actcgcctga ctaccgctga actgccagtg attgcttcac
      601 tggggattgc cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt tctaccggtg
      661 atgaactcca gttgcccggt cagccactgg gcgatggcca aatctacgat accaaccgtc
      721 tcgccgtaca cctgatgtta gaacagttgg gatgcgaggt aatcaactta gggattatcc
      781 gcgacgatcc acatgccctg cgcgccgcat ttattgaagc cgacagccag gcggatgtgg
      841 tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacc attcttgaag
      901 agctggggga gatcgccttc tggaagctgg cgattaaacc gggtaaaccg ttcgcgttcg
      961 gtaaactttc taatagctgg ttctgcggcc tgccgggcaa cccggtttca gcgacgctga
     1021 ccttctatca actggtacag cctttgctgg caaaactaag cggcaacacc gccagcggcc
     1081 tgcccgcgcg ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca ggacgtcttg
     1141 atttccagcg cggcgtgctg caacgcaacg ccgacggtga acttgaagtg acgaccaccg
     1201 gacatcaggg ttcacatata tttagctcct ttagtctcgg caactgcttt atcgtgctgg
     1261 aacgcgatcg cggcaatgtg gacgtgggcg aatgggtgga agtagaaccg tttaacgcgt
     1321 tgttcggagg cctgtaatgg cggaactcag cgatcaggag atgctgcgct acaaccggca
     1381 aatcattctg cgcggttttg attttgacgg tcaggaggcg ctaaaagatt ctcgtgtgct
     1441 ggtagtcggc ctcggtggac tcggctgcgc agcttcgcag tatctggcaa gcgccggtgt
     1501 cggcaacctg acgctgctcg acttcgacac ggtttcgctc tcgaatctgc aacgccagac
     1561 actgcacagt gatgccacgg tcgggcaacc gaaggtggaa tccgcccgtt acgccctgac
     1621 gcggatcaac ccacatatcg cgattacgcc agtcaatgca ctgctagatg acgcagaact
     1681 tgcagcattg attgctgaac acgatctggt gctcgactgt acggataacg ttgcggtacg
     1741 taatcaactg aacgcaggct gttttgccgc gaaggtgccg ctggtttccg gcgcggcaat
     1801 tcgtatggaa gggcaaatca cggtcttcac ttatcaggac ggcgaaccgt gctatcgctg
     1861 ccttagccgt ttgtttggcg agaacgcatt aacctgcgta gaagcaggcg tgatggcacc
     1921 gttgatcggc gtaattggtt cgttgcaggc aatggaagcg atcaaactgc tggcaggtta
     1981 tggcaaaccg gccagcggga aaatcgtcat gtacgatgcg atgacctgtc agtttcgcga
     2041 aatgaaactg atgcgcaatc cggggtgtga ggtgtgcggg cagtaattgc cacctccagg
     2101 cctgataaga cgcgacaagc gtcgcatcag gcatttgctc cagacgccag atgcggcgtg
     2161 aacgccttat ctggcctacg gttcgggcac agatttccaa gcgcggcagg catcggtgca
     2221 ctccgccacg tcatcagcaa tttatgagga tgcagaacgt ccggttaaat cgacgttctg
     2281 ccaaacgctc ccagccagtc ctgttcaaac ttcgccacag cggcatcaac cgccggatag
     2341 ctaatcatct gttgtgccac atccagtggc agcgtaattg attcacatcc tgccagtaag
     2401 cagtccagcg cctgacgcgg ggttttgaaa ctcgctgcca gcactttcgc ctgcggcgca
     2461 tgcattttca ataactggtg taagtcggtc acagtctgaa tgccgctacc gccctgagca
     2521 tcaatacgat taacgtaagg cgcaacatat tccgcacctg ccagcgccga cagcagccct
     2581 tgtgctgcgc catataccgc ggttcccagc gttggaatcc cttccgcttt taacatctta
     2641 atagctgcca gcccctcggc ggtcaccgga actttcacca cgatatccgc aataatagaa
     2701 cgcaatttac gtgcgtcatt caccatccct tcagcagtgg tagccattac ctgggcaaac
     2761 agacgcccct gaccgcccat cgcttcatga agttgcggaa gcacaacatc cagcggtttt
     2821 ttacccgcgg cgataatgct tgggttagtg gtcacacccg ccagcggaaa aatacgtgac
     2881 agcgccttca ccgcaacaac gtctgaagta tccagataca gttccataac catcctcaaa
     2941 atgtactttt tgtctgaatg tagactatca cgggaaaact ctctgaacag ttgacgtaca
     3001 tcaatctaac tttcattcga aagtatttta atgtttgaac gaaacagaga ggcaactatg
     3061 attttcaata ttcagcgcta ctcgacccat gatggccccg gtatccgcac ggtcgtattt
     3121 cttaaaggct gttcgctggg ctgccgctgg tgtcagaacc cggaaagccg cgcccgcacg
     3181 caggatctgc tgtatgacgc acgactgtgt ctggaaggct gcgagctgtg cgctaaggcc
     3241 gcgccggaag tgattgagcg cgcgctgaat ggtttgctta ttcatcggga aaagttaacc
     3301 ccggagcatc tgacggcgtt aaccgactgc tgtccgacac aggcattaac cgtgtgtggt
     3361 gaagtgaaaa gcgttgagga gatcatgacg accgttctgc gcgataaacc gttttatgat
     3421 cgcagcggcg gcggtttaac gctttcgggt ggtgagccct ttatgcagcc ggaaatggcg
     3481 atggcgctac tgcaagccag ccacgaggca ggcattcata ctgcggtaga aacctgtctg
     3541 catgtgccgt ggaaatatat cgccccttct ctgccctata tcgatctgtt tcttgccgat
     3601 ttaaaacacg ttgccgacgc gccgtttaaa cagtggaccg acggtaacgc cgccagagtg
     3661 ctggataacc tgaaaaaact cgccgcagcg ggcaaaaaaa tcattatccg cgtgccgctg
     3721 attcagggct ttaatgccga cgaaacctct gtaaaagcca ttaccgattt tgccgccgac
     3781 gagctgcacg ttggcgaaat tcattttctg ccctaccaca cgctgggcat caacaaatat
     3841 cacttactta atctgcccta tgacgccccg gaaaaaccgc ttgatgcgcc agaactgctc
     3901 gactttgccc agcagtatgc ctgccagaaa gggttaaccg cgaccttacg aggataacaa
     3961 ccatgaccac actgaaactg gacacgctca gcgaccgcat taaagcgcac aaaaatgcgc
     4021 tggtgcatat tgtgaaaccg ccagtctgta ccgagcgcgc gcagcactat accgagatgt
     4081 atcaacaaca tctcgataag ccgatcccgg tacgtcgcgc gctggcactg gcgcatcacc
     4141 tggcgaatcg caccatctgg atcaaacacg atgagttgat cattggcaac caggcaagcg
     4201 aagttcgcgc cgcgccgatc ttcccggaat atactgtctc gtggatcgaa aaagagattg
     4261 atgatctggc agatcgtccc ggtgctggct ttgcggtgag cgaagagaac aaacgcgttc
     4321 tgcatgaagt gtgcccgtgg tggcgcggtc agaccgtaca ggatcgctgc tacggcatgt
     4381 ttaccgatga gcaaaaaggt ctgctggcga ccggaatcat taaagcggaa ggcaatatga
     4441 cctccggcga tgcgcacctg gcggtgaatt tcccgctgct gctggaaaaa ggtcttgatg
     4501 gtctgcgtga gaaagtagcg gaacgtcgct cgcgcatcaa tctgacggtg ctggaagatt
     4561 tacatggcga acaattcctg aaagcgattg atatcgtgct ggtggcagtc agtgaacaca
     4621 ttgaacgttt cgctgccctg gcgcgtgaaa tggctgctac cgaaactcgc gaaagccgtc
     4681 gcgatgaact gctgacgatg gcagaaaact gcgatcttat cgcccaccag ccgccgcaga
     4741 ctttctggca ggcgctgcaa ctgtgttact tcatccaatt gattttgcag atcgaatcta
     4801 acggtcactc ggtatcgttt ggtcgtatgg accagtatct ctacccgtac tatcgccgcg
     4861 acgttgaact caaccagacg ctggatcgcg aacacgccat cgagatgctg catagctgct
     4921 ggctgaaact gctggaagtg aacaagatcc gctccggctc acactcaaaa gcctctgcgg
     4981 gaagtccgct gtatcagaac gttaccattg gcggacaaac tctggttgat ggtcaaccaa
     5041 tggacgcggt gaatccactc tcttacgcga tcctcgaatc ctgcggtcgc ctgcgttcca
     5101 ctcagcctaa cctcagcgtg cgttaccatg caggaatgag taatgatttc ctcgacgcct
     5161 gcgtacaggt gatccgttgc ggcttcggga tgccggcgtt caacaacgac gaaatcgtga
     5221 tcccggaatt tattaaactc ggtattgaac cgcaggacgc ttacgactac gcagcgattg
     5281 gttgtatcga aaccgccgtc ggtggcaaat ggggctatcg ctgtaccggc atgagcttta
     5341 tcaacttcgc ccgcgtgatg ctggcggcgc tggaaggcgg gcgtgatgcc accagcggca
     5401 aagtgttcct gccacaagaa aaagcgttgt cggcaggcaa ctttaacaat ttcgatgaag
     5461 taatggacgc gtgggatacg caaatccgtt attacacgcg taaatcaatc gaaatcgaat
     5521 atgtcgtcga caccatgctg gaagagaacg tacacgatat tctctgctcg gcgctggtgg
     5581 atgactgtat tgagcgagcg aaaagtatca agcaaggcgg cgcgaaatat gactgggttt
     5641 ctggcctgca ggttggcatt gccaacctcg gcaacagcct ggcggcagtg aagaaactgg
     5701 tgtttgaaca gggtgcgatt ggtcagcaac agctggctgc cgcactggcg gatgacttcg
     5761 atggcctgac tcatgagcaa ctgcgtcagc ggctgattaa cggtgcgccg aagtacggca
     5821 acgacgatga taccgtcgat acgctgctgg ctcgcgctta tcagacctat atcgacgaac
     5881 tgaaacagta ccataaccca cgctacggtc gtggtccggt tggcggcaac tattacgcgg
     5941 gtacgtcgtc tatctccgct aacgtaccgt ttggcgcgca gactatggca acaccggacg
     6001 ggcgtaaagc ccacaccccg ctggcagaag gcgcaagccc ggcttccggt actgatcatc
     6061 ttggccctac tgcggtcatt ggctcggtgg gtaaactgcc tacggcagcg attctcggcg
     6121 gcgtgttgct caaccagaaa ctgaatccag caacactgga gaacgaatct gacaagcaga
     6181 aactgatgat cctgctgcgc accttctttg aagtgcataa aggctggcat attcagtaca
     6241 acatcgtttc ccgcgaaacg ctgctggagg cgaaaaaaca tccggatcag taccgcgatc
     6301 tggtagtgcg tgtcgcgggt tattccgcct tcttcaccgc gctctctcca gacgctcagg
     6361 acgatatcat cgctcgtact gaacatatgc tgtaatctct tcggcccggc tgcggcgtcg
     6421 ccgggctaaa tcattcacat catcaatttc atccttactt tcattcgaaa tataatttgt
     6481 gctctgcgtc acattgttat tacactgtta cagtttcatt ttcgagccag gagcacacct
     6541 atgagcgtaa aagttatcgt cacagacatg gacggtactt ttcttaacga cgccaaaacg
     6601 tacaaccaac cacgttttat ggcgcaatat caggaactga aaaagcgcgg cattgagttc
     6661 gttgtcgcca gcggtaatca gtattaccag cttatttcgt tctttcctga gctaaaggat
     6721 gagatctctt ttgtcgcgga aaacggcgca ctggtttacg aacatggcaa gcaactgttc
     6781 cacggcgaac tgacccgaca tgaatcgcgg attgttattg gcgagttgct aaaagataag
     6841 caactcaatt ttgtcgcctg cggtctgcaa agtgcatatg tcagcgaaaa tgcccccgaa
     6901 gcatttgtcg cactgatggc aaaacactac catcgcctga aagctgtaaa agattatcag
     6961 gagattgacg acgtactgtt caagttttcg ctcaacctgc cagatgaaca aatcccgtta
     7021 gtgatcgaca aactgcacat agcgctcgat ggcattatga aacccgtcac cagtggtttt
     7081 ggctttatcg acctgattat tcccggtcta cataaagcaa acggtatttc gcggttactg
     7141 aaacgctggg atctgtcacc gcaaaatgtg gtagcgattg gcgacagcgg taacgatgcg
     7201 gagatgctga aaatggcgcg ttattccttt gcgatgggca atgctgcgga aaacattaaa
     7261 caaatcgccc gttacgctac cgatgataat aatcatgaag gcgcgctgaa tgtgattcag
     7321 gcggtgctgg ataacacatc cccttttaac agctgacctc tctaccggag ccgtgcgctc
     7381 cggttttccc ttctttttac atcctgtaca tcacatcaaa tcattaaact tgcattaact
     7441 tatttataac ttatattgtc acatgcaaat cagaatggtt tcgaatgaaa gttaatgaga
     7501 ggtggttatg gcttctactt ttaccagcga cacattgcct gccgatcaca aagcagctat
     7561 ccgtcagatg aagcacgcgc tgcgggcgca gcttggcgac gtccagcaga tctttaatca
     7621 gctaagcgat gacattgcca cgcgagtggc tgaaatcaac gcactcaaag cacagggcga
     7681 tgccgtctgg ccggtgctgt cttatgccga tatcaaagca ggtcatgtta ctgcagagca
     7741 gcgcgaacag attaaacgtc gcggttgtgc ggtgataaaa ggccatttcc cccgcgaaca
     7801 agcgctaggc tgggatcagt cgatgctgga ctatctggac cgcaaccgct ttgacgaggt
     7861 ctacaaaggc cccggcgata atttcttcgg gacgctcagc gcttcacgtc ccgagattta
     7921 ccccatctac tggtcgcagg cgcaaatgca ggcccgccag agtgaagaaa tggcgaatgc
     7981 gcagtcgttt ctcaatcgtc tgtggacatt tgaaagtgat ggaaagcaat ggtttaaccc
     8041 ggatgtgagc gtcatctacc ctgaccgtat ccgccgccgt ccgcccggaa cgacctccaa
     8101 aggtcttgga gcgcataccg actccggggc gctggaacgc tggctgcttc cagcgtatca
     8161 gcgcgttttc gccaacgtct ttaatggcaa tctggcgcaa tatgatccct ggcatgcggc
     8221 acatcgtacg gaagttgaag agtacacggt ggacaacacc accaaatgtt ccgtgtttcg
     8281 gacattccag ggctggacag cgctctctga tatgctgcct ggtcaggggc tgctgcacgt
     8341 tgtgcccatt cctgaagcca tggcgtacgt actgttacgt ccgctgcttg atgatgtgcc
     8401 ggaggatgaa ctgtgcggcg tagcgcccgg aagagtgttg ccggtatcag agcaatggca
     8461 tccactgtta attgaggcgt taaccagcat tccaaaactc gaggccggag actccgtctg
     8521 gtggcactgc gacgtcatcc attccgttgc ccccgttgaa aatcaacagg gttggggcaa
     8581 cgtgatgtac attcctgcgg caccgatgtg cgagaaaaat cttgcctacg cgcacaaggt
     8641 gaaggccgca ctggaaaaag gcgcatcgcc gggcgacttc ccgcgcgagg actatgaaac
     8701 aaactgggaa ggacgcttta cgcttgccga cctcaacatt cacggtaagc gagcgttggg
     8761 catggatgtt tgagagttaa tggaagtgaa ttgatgtggg attaagaaaa tcgattcgac
     8821 gttactcaga ttgctgacaa gtgcgctttc ttaatgcctg atgcgctacg cttatctggc
     8881 ctacaaagtc ctgcagattc aatatattac aggtact
//
LOCUS       DAPGWD010000170         8892 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPGWD010000170 DAPGWD010000000
VERSION     DAPGWD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.171"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(149..289)
                     /locus_tag="RHX88_003805"
     CDS             complement(149..289)
                     /locus_tag="RHX88_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232441.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            385..1101
                     /gene="arcA"
                     /locus_tag="RHX88_003806"
     CDS             385..1101
                     /gene="arcA"
                     /locus_tag="RHX88_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDV2232442.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1161..2513)
                     /gene="creD"
                     /locus_tag="RHX88_003807"
     CDS             complement(1161..2513)
                     /gene="creD"
                     /locus_tag="RHX88_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDV2232443.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(2571..3995)
                     /gene="creC"
                     /locus_tag="RHX88_003808"
     CDS             complement(2571..3995)
                     /gene="creC"
                     /locus_tag="RHX88_003808"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDV2232444.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3995..4684)
                     /gene="creB"
                     /locus_tag="RHX88_003809"
     CDS             complement(3995..4684)
                     /gene="creB"
                     /locus_tag="RHX88_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDV2232445.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4697..5170)
                     /gene="creA"
                     /locus_tag="RHX88_003810"
     CDS             complement(4697..5170)
                     /gene="creA"
                     /locus_tag="RHX88_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDV2232446.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5381..6250
                     /gene="robA"
                     /locus_tag="RHX88_003811"
     CDS             5381..6250
                     /gene="robA"
                     /locus_tag="RHX88_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDV2232447.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6247..6894)
                     /gene="gpmB"
                     /locus_tag="RHX88_003812"
     CDS             complement(6247..6894)
                     /gene="gpmB"
                     /locus_tag="RHX88_003812"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDV2232448.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6946..7458
                     /gene="yjjX"
                     /locus_tag="RHX88_003813"
     CDS             6946..7458
                     /gene="yjjX"
                     /locus_tag="RHX88_003813"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDV2232449.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(7511..8859)
                     /locus_tag="RHX88_003814"
     CDS             complement(join(7511..8379,8379..8859))
                     /locus_tag="RHX88_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2232450.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPQALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNMVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKK
                     PPGAGEKTKVIQSLKCGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIF
                     HEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAE
                     NILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVK
                     RMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSALADKGLVQSMSRKGNCLDNAVMEN
                     FFGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG"
BASE COUNT         2171 a         2391 c         2132 g         2198 t
ORIGIN      
        1 tgatgcaact agttggatta ttaaaataat gtgacgaaag ctagcattta gatacgatga
       61 tttcatcaaa ctgttaacgt gctacaattg aacttgatat atgtcaacga agcgtagttt
      121 tattgggtgt ccggcccctc ttagcctgtt atgttgctgt taaaatggtt aggatgacag
      181 ccgtttttga cactgtcggg tcctgaggga aagtacccac gaccaagcta atgatgttgt
      241 tgacgttgat ggaaagtgca tcaagaacgc aattacgtac tttagtcatg ttacgccgat
      301 catgttaatt tgcagcatgc atcaggcagg tcagggactt ttgtacttcc tgtttcgatt
      361 tagttggcaa tttaggtagc aaacatgcag accccgcaca ttcttatcgt tgaagacgag
      421 ttggtaacac gcaacacgtt gaaaagtatt ttcgaagcgg aaggctatga tgttttcgaa
      481 gcgacagatg gcgcggaaat gcatcagatc ctctctgaat atgacatcaa cctggtgatc
      541 atggatatca atctgccggg taagaacggt cttctgttag cgcgtgaact gcgcgagcag
      601 gcgaatgttg cgttgatgtt cctgactggc cgtgacaacg aagtcgataa aattctcggc
      661 ctcgaaatcg gtgcagatga ctacatcacc aaaccgttca acccgcgtga actgacgatt
      721 cgtgcacgca acctgctgtc ccgtaccatg aatctgggta ctgtcagcga agaacgtcgt
      781 agcgttgaaa gctacaagtt caatggttgg gaactggaca tcaacagccg ttcgttgatc
      841 ggccctgatg gcgagcagta caagctgccg cgcagcgagt tccgcgccat gcttcacttc
      901 tgtgaaaacc caggcaaaat tcagtctcgt gctgaactgc tgaagaaaat gaccggccgt
      961 gagctgaaac cacacgaccg tactgtagac gtgacgatcc gccgtattcg taaacatttc
     1021 gaatctacgc cggatacgcc ggaaatcatc gccaccatcc acggtgaagg ttatcgcttc
     1081 tgtggtgatc tggaagatta atcggcttta ccaccgtcaa aaaaaacggc gctttttagc
     1141 gccgttttta tttttcaacc ttatttccag atacgtaatt gatcgtccat tgtaacttct
     1201 ttactggctt tcattttcgg cagtgaaaac gcataccagt cgatattacg ggtcacaaac
     1261 atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc gctatcggca
     1321 gagttgagca gtccccacat cacaccatcc agcaacaaca gcgcgagggt aaacaacatg
     1381 ctgttgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa cgccccaatc
     1441 agactggcga ttatccatgc cacggtaaaa ccggtatgtt cagaaagcgc cagcaagagc
     1501 aaataaaaca tcaccaatga aagccccacc agcaaatatt gcattgggtg taagcgttgc
     1561 gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gcgcaatcag cagaatggca
     1621 tacttagtcg cccggtcagt taattggtat tgatcggctg gcgtcgtcac tgcgacgcta
     1681 aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc accgagatta
     1741 tttgcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctaac ttcccgtttg
     1801 gctggcaaaa aatcacctaa aaaactggga tgcggccagt tgctggttaa ggtcatttcg
     1861 ctattacgcc cggcaggcac cacagaaaga tcgccggtac cgcttaaatt cagggccata
     1921 ttcagcttca ggttctgctt gcgccagtcc ccttcgggta aagggatatg cacgccctgc
     1981 ccgccttgct ctaacccggt accgggttcg atggtcagcg ctgttccgtt aacttcaggc
     2041 gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa tggcttgcct
     2101 aaggtgatat ttggtgcgtc gagttcgcta agacgcgaaa catcgaaatc ggcttttagc
     2161 gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc ttcaacgttc
     2221 tgattgccat caaccatcaa tgactcaggt aaccaaaaat ggataaaact tcgtttccgc
     2281 tccacggttt tatcgtcttc ctgcaccgta taaagctcgg tcacaggaat agcgatgagc
     2341 ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc cacatcgcta
     2401 cggtaatcgg cacgttcgac aatcacctgc cgaatcagca ttatcggaat caacaacagc
     2461 aatactgcac caaacaaggt ggtcattttc cagaacaggg gggatttcaa catagtcttc
     2521 tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag ctatgtgaag
     2581 tgacggtgaa gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg cagcgtgact
     2641 tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc gctgcttttt
     2701 tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag cgcgtagtca
     2761 ggaataccac taccggtatc cagcacctta agggcgacgt gttcctgatc cacttcggcg
     2821 cttagcgtta tgcgaccgct ctcgggggta aaatcgatgg cgttatccag taaattcccc
     2881 agcgcctgct ccagtaacgc cggttcagca gcaacattaa cctcagtggg cataacatgt
     2941 agagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg gcgaaataat
     3001 gccgccacat caacagcagt cagaacgact tcctgacgat tctccagtct tgcctggcgt
     3061 agtaacgttt ctaccaatgc ctgcatacgc gcattttgcg tcagaatgtt gtcggtaaaa
     3121 cgagccacca cttccggtgg cggaccttcg cgtaaaattt ccgccgcgcc acgaatcgcc
     3181 gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat atagtttttc
     3241 ccttccagct tcacgcgcat actttccagc gcctgcgcaa gcttacgcaa ctcgctactg
     3301 ccgagttcag gaagaggaac gggcttattg tcagtgacgg aatcggcata gcgagtgagc
     3361 cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag tgcaatcccc
     3421 agcaaaatgg cgctggccca taaaattcgc cgctcgctac gcttaatgac cggagccatc
     3481 gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgtc cataatcggc
     3541 gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt gctgcgcgca
     3601 ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc ctgcccaacg
     3661 gctttatttg ccgaatcgaa caatacttta ccctgcgaat cggtcatata gacatgatat
     3721 tcattgcgca ctttgttaat gccaccgata ttggcgcgaa acgggcgatg ttgtagctga
     3781 ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc gggacgcgcc
     3841 agctccgcca gcaacgttgc ggtatcgatt aacgtcccct ccgttgctct tcgcacgccc
     3901 ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc caccagtaaa
     3961 aaatagccca gcagcaaccg catgccgata cgcattacag gcccctcagg ctatatccca
     4021 tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc ttggcacgca
     4081 acgttttaat gtgggtatca acggtgcgat cgtaggtgtc ctgcgcatct tcccacacgc
     4141 tatccatcag ttgctggcgg gaccagacgc ggcctggcga cttgagtaac gtcttcagca
     4201 ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc tgcgccgcgg
     4261 gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgacgag aacttcttca
     4321 cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac ggtttagcca
     4381 catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca cttcgggccg
     4441 tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac aattcaaagc
     4501 cgctgatatc aggcaaacca acgtcgagaa tcatgacgtc gggaaccagc tggcgagctt
     4561 tatccagcac cggcaagcct cgctcaaaga cctcgacggc aaaaccttcc tgctgcaaca
     4621 tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc gtttcccgtt
     4681 gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta atcgcgtttt
     4741 tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg ttgcgtttgg
     4801 cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt tttttgaata
     4861 ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc ggcccgactt
     4921 gctgacaaga aatggccgca tcggaggtat cttccgccag gcccaatccc cctttaatac
     4981 ccccggtttt cgcccggctc acataacagg tgacattttt cacatcggga tcgtcaaagg
     5041 cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg accgaaccaa
     5101 tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg atcaaatgct
     5161 tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg tcacagtaaa
     5221 taacatattg attaagaaca aaatctcaat acttttattt ccgtcaagcc ctaaaacata
     5281 ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta tccaattacc
     5341 tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg ccggcattat
     5401 tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc tcgacaatgt
     5461 agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag atgtcactgg
     5521 ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg tcgcactacg
     5581 cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact ctcaacagac
     5641 atttacccgc gcattcaaga agcagtttgc ccagactcct gcactttacc gccgttctcc
     5701 tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtgaattca caatgccaga
     5761 gcacaaattt gtcaccctgg aagatacgcc gctgattggt gttacccaga gctactcctg
     5821 ttcgctggag caaatctctg atttccgcca tgaaatgcgt tatcagttct ggcacgattt
     5881 tctcggcaac gcgccgacca ttccgccagt gctctacggc ctgaatgaaa cgcgtccgag
     5941 tcaggataaa gacgacgaac aagaggtatt ctataccact gcgttagccc aggatcaggc
     6001 agatggctat gtactgacgg ggcatccggt gatgctgcag ggcggcgaat atgtgatgtt
     6061 tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacagtat acggaacgtg
     6121 catgccaatg ctcaatctga cgcgccgtaa aggtcaggat attgagcgat actacccggc
     6181 agaagatgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc tgattccgat
     6241 ccgtcgttaa cgctgcaact catctaatgc aggggcgtcc agatgcgaga tgtcccctgc
     6301 ggtttcaaca acccagccag acgccagcca caggctttcc tgataatcca cgcgcgaaat
     6361 agaacagtta cgcagacgta agcggcgctc tgcccatgct ggtaatccga gaatcgtact
     6421 caccagacat cccagtgcaa taccatggct caccagcagc ggtcggcttc cctgcggtaa
     6481 gtcccggcaa gattccagtg ccgcattgac gcgatcgctg agctcctgca ttgactcgcc
     6541 ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt tctcttcttc
     6601 ttcggtcaga gaatcgatat gtcttttttc cagcacaccc atgtttaatt cacgcaggcg
     6661 agaatcaaag atgatgtcac agccgcaggc ctgggcgatg atttccgccg tacgccgggt
     6721 gcgtcctaaa tcgctactga taatatgcgt aatgccaagc tctttggcac gggttgccac
     6781 ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg tcagactggc cctgaatacg
     6841 tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta acatgctttt
     6901 tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca ccaagttgtc
     6961 tgtgcgacca ccaatcccgc taaaattcag gccattctgc aggcatttca cgagatcttc
     7021 ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc ggaacagccc
     7081 tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc ccgccgttta
     7141 cttccagagg ctgatttttg ggtggcgatt gaagctggca tcgatggtga cagcactttc
     7201 agttgggtgg tgattgaaaa caccagccag cgcggcgagg cgcgttcggc tactttgccg
     7261 ttacctgcgg tgattctgca gaaagtgcgt gaaggtgagg cgcttggtcc cgtgatgtcg
     7321 cgttataccg gtattgatga gattggtcgt aaggaagggg cgatcggcgt gtttaccgcc
     7381 gggaaactca ctcgcgccag cgtctatcac caggcggtga tcctggcgct gagtccgttt
     7441 cataatgccg tgtattaagc gcctgatgcg acgctgcctg aactgaaccc caaaacctgg
     7501 acagtttcag ttaaccggct ttttgatact cagtccggta ttctaccggg ctgagtcccc
     7561 caagactgag ttttattctt ttttgattcc agtaagttat gtattcgtta acagcatttt
     7621 ccagctcttc tacacttctg tagtcccggc gatagtacat ttcttctttc aggtgaccaa
     7681 agaaattctc cattaccgca ttatcaaggc agttgccctt gcgcgacatg ctctgcacta
     7741 accctttgtc tgccagggca gactgataac tttttatccg gtaatgccac ccttgatcac
     7801 tgtggagcag cggtttttct ccttctgcaa ggctttccag ccccttattt aacatgcgct
     7861 tcaccagctc ctctgtaggc ctgcaggcag tttcccacgc cacgatttcc ccgttaaaca
     7921 aatcaagtat cggggacagg tacagttttt gtccgccagc cctgaattcg gtgatatctg
     7981 tcacccattt ctcacaggga gcttccgctt tgaactgccg ctgaaggata ttttctgcag
     8041 cgagtcccat atttccccgg taagaccggt atttcttcag gcgcacagga gacttcaggc
     8101 cgagctgttg catcagtttt cgcaccgttt tgccactgaa cttaagacct cttttctgaa
     8161 gctcacaatg gatccgccgg tacccataac atccccggtg ttcatggaag atggaggcta
     8221 tgagctgttt cacatcagca tacttgtcct tagccttctg cagtgacagc tggtagtaca
     8281 gcgtgcttct ggccagccct gcagccttta acaggtcaga ctggcagtgt ccgcacttca
     8341 ggctctggat gactttcgtt ttttctccag ctcctggcgc tttttttcca ggagatgctc
     8401 ctgcatggct ttcagatatg cattttctgc gcgaagataa cgcagctcat tcttcatctc
     8461 ttccggcgtc atgtttgcct cgttttcatg ggtatcatca gaacgggtca tgcttttctc
     8521 cttcctgcgc ctgataaagg cctcattacc agatttgcgg tagcctttta tccagttttt
     8581 aaccatgttg tggctgggaa tgttaaaccg ggcagctacc cggggtagag actcagaatg
     8641 cccgagagca taaagaacca cgcaaagttt atcttcagga gaatagctac gcttgtgtct
     8701 gcaatccagt gcctgaggac cgtgaagaag aaaaagatta atccagtgtg agaggctggt
     8761 gtgagaaagt tgaaagagtt ttgccgtaga aatgatgcct gcatgaccgg ccaggtaatg
     8821 atttacagcg gcaagtttta cttcaaatga atgtttcata aactgcacct tcagatgtca
     8881 gattatgtcc aa
//
LOCUS       DAPGWD010000171         8855 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPGWD010000171 DAPGWD010000000
VERSION     DAPGWD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.172"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(128..3490)
                     /gene="mscK"
                     /locus_tag="RHX88_003815"
     CDS             complement(128..3490)
                     /gene="mscK"
                     /locus_tag="RHX88_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDV2232451.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(3618..4265)
                     /gene="acrR"
                     /locus_tag="RHX88_003816"
     CDS             complement(3618..4265)
                     /gene="acrR"
                     /locus_tag="RHX88_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDV2232452.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            4407..5600
                     /gene="acrA"
                     /locus_tag="RHX88_003817"
     CDS             4407..5600
                     /gene="acrA"
                     /locus_tag="RHX88_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDV2232453.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            5623..8772
                     /locus_tag="RHX88_003818"
     CDS             5623..8772
                     /locus_tag="RHX88_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV2232454.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         2119 a         2280 c         2320 g         2136 t
ORIGIN      
        1 cgctcctgca atacattgaa ttcttgcggt ttcgtaggcc ggataaggcg gtcacgccgc
       61 atccggcatg aacaacgcgc actttgtcag taatctcggg gcaactaaat gtcgccacct
      121 ttggttatta ccctaccgct ggcgtcgggt catcgccttt gtagtcgcgt ttgacttccg
      181 tcacctcatc gcccttctcg ttgtgcagat gcacttcaag ctggttaaag gcaatgttga
      241 tgtcgttttc acggcacagc tgatcgatag tacggttcag ctcatcgaca gtacgactac
      301 ggtcacgcag ttcacgcaca tacagacgca gctcatgatc caacgtgctg gcaccaaacg
      361 ccgtaaagaa gacttccggc attggttcgt gcatcaccct tgggtgctca gtcgccgcct
      421 tcagtaacac tttacgcact ttttccagat cggagccata ggccacgccg agacggatca
      481 ccagacgcgt agtagtgtca gtcaacgacc agttgatcag acgctcggta acaaacgctt
      541 tgttcgggat gatcacttct ttgcgatcga aatcggtaat cgttgtcgca cgaatacgga
      601 tcttacttac cgtccccgag aagctaccaa tggttaccgt atcaccaata cgcaccggac
      661 gttcgaacag gatgatcaaa ccagagacaa agttaccgaa gatctcctgt aagccaaaac
      721 caagacctac ggataatgct gccgccagcc actggagttt atcccacgag acgcccagcg
      781 atccgaacac cgtcatcgca ccaacggcaa taatgatgta gttaaggatg gtagtaatgg
      841 catacgacgc gccctgacgc atattcagtc gcgagagcac cagcacttcc agtaaaccag
      901 gcaggttgcg aatcaacgcc caggccacca ttgaggcgat aatcgcaaac aacagactgc
      961 ccatggtgac gtttttcacc accgcagcgc cagcttcagt gccgttgtaa tgccagagcg
     1021 tgatgctgtc gagatagctg aacacggtga tcaaatcgga ccaaattgcc cagaacatga
     1081 caccgaacag cgcaaacatc agcaacatgg taatacgcag cgtctgctgg ttaacctgtt
     1141 ccagtgcaat ggtgggttct tccggcggtt cggcaccttc tgcgccctct ttcaccagat
     1201 tctgccgacg cgccagcgca cgacgccagg cgatacgccg cgccgctacg cttaagccgc
     1261 gcaataccgt ctggtagagc aagttccaga tgatcaccag ataaacggtt tcaatccaac
     1321 gcccggagag gcgcagcgtg gtgtagaaat aacccgtggc agtcagcacc atcaatgcaa
     1381 tcgggataat cgacagcacg gtaatggtga ccagtcgcat ggtgtgcgac tctttatcac
     1441 gccagctttc gcggcacatt ggccatacca ggaaggcaat cagcagcagg ttgaagaaaa
     1501 tcatcgcctg ccccagcaca tcatccatca gatgcagcgg ggaaagttct gccaccacag
     1561 accagaaatg gataggcagc aacgcgagac tgatgcggac aatttgccga cgccagtggc
     1621 tggtctgctg ctccggcatg ccgaagtgac gcacggcaac gccatttttc tccagtacct
     1681 tccagcacag gccaaacacc agccagaaaa tcgccagttt tttactgaac gaccatagca
     1741 gttcgctgat gttgagctgc atggtcaaca gaatcaggcc aaccgcgaga ataatcaggc
     1801 acaccggcag cgcgcggatc aggtcgataa ggatcgcttt tggtgtgttg agctggctat
     1861 cgttacgcag ggaacccaca gcggaagcca gtttctgttg atacgctttc agccagccca
     1921 gacgccagtg gatcagcccg gcaatcaaca gcagcggcaa accggcgagg aaagcgataa
     1981 aaacggcagg ccaggctttt tcccagttca ccgtgatttt catcgactta aattcatctt
     2041 tcaggctttg cgggaacgct ttgatccagt cccagtccat tggacggtta ctgttcaccc
     2101 aaaagatttg ctgagtcagg atggatttca ggtttttcga tacgctcatt aactgctgct
     2161 ggttgatttg caggttaatg gccatcatca gctggttacc caactgtttg ttgagttgat
     2221 ccagcaattc gcgacgcata tcaaccactt gcaataacgc atcgtgaact tcgctgttga
     2281 cttcgttggt gtgaccttct tccagtttgt tgacgaacgc atcgctctgg aagagtgcat
     2341 cacgctgctg gttaacttca aactgttcga gacgcaaatc cgcgatgcgg ttggtcatgt
     2401 tttccagttc atccgccgag ggcagcgttt gttgttgctg gtaaaggata cgagacaaca
     2461 gcaggctgcc cttcaggacg gcaatctgct ctttaatatt gcgttccgat tgcagcgccc
     2521 gctccagcca gtttttgact ttaatgtttt gctgcatcaa ctgattaccg ttttcagtcg
     2581 cggtaatcag acgctgactt aactgctggt taatttccag ttcctgtttc accagcggat
     2641 tagcctgaat acgcgcggct tcatccgggg agacggcttc ctgcgccgtt ttttcagtta
     2701 aagtcaggcg cttgctgttt accgcttctt gcaacagctg caactggtgc tccagacggg
     2761 cgctgttcgc cgtcacgtaa tcacgttgct tttgcaaggt atcctgcaag acggtgttcc
     2821 cttccaggct tttacgctgc tggtcaatct ccgcattcag caacgcctgc tgagcctgca
     2881 ttaacacttt ctggctggga cgtaaggcag tctcgccgac atcagtccca tccagacgac
     2941 tgcgaatttg ttgcagctgc tgcgaagcgt tatacatcgc attttgcacg cgttcgggct
     3001 gcgtctgtaa cgaaaccagc tggctgttat aagacgccag atcgttttgt gcgttttgca
     3061 aatcgtccag cgcctgggca acgcgagttt ccagctggcg caacgaaagc gtactcagaa
     3121 ttttgcgcgt ttcttcgtcg ttatcgacat cgctaagtgc tgttaacgcc gcggtcgcct
     3181 ggcgcatttt ttccggcgct tcagcgactt tttgccgtag ctgaactgtc tcttctttta
     3241 tgcgatcgat tttatcgagg gtggctaatg tatctgtcag atcctgctgc accagtttgt
     3301 cctgagcaga aagatctttt tgtttattta gtgagtcaag ttgcgcctgc aggtccgctt
     3361 ttgtcggcag atcaccattc gatgacgccc gcgcaaacgc cgtgttctgg cataacaaga
     3421 caaaaacaaa agcaataaat gctgaaaaaa caaaatgccg tgatcgtttg taatactgga
     3481 acatagtcat gatgaatgaa ggtttctgaa cctgaagaac gacctgaaaa agtcaaaccg
     3541 caagaatatc acgacgcagt gaaccagaat agcaacgacg aaaatgtcca ggaaaaatcc
     3601 tggagtcaga ttcagggtta ttcgttagtg gcaggattac gaagcgtggg gcacaggaga
     3661 tacatctcca gtaagatggc gacgtaatcg cgggcttctt ttttaagatc aaaagattgc
     3721 ggggcaaaga gccagttttc catcaggccg gaaatatagc cgcgcataat aattgctgcc
     3781 cgacgcgtca ttaaatccgc aggcaacatt ttcgcttcaa tacaatgttt taacgtttgt
     3841 tctatacggt cataactttc cagacagaga ttacgttgtg cctgttgcac aacagccatt
     3901 tctccgacaa attcgcattt gtggaatata atctccatca ataatcgacg ccgttcttct
     3961 gtcaccgtgg attcaagaac atgaattaat atctctctta atactgagag tggatcgcca
     4021 gggaattttg cctgatactc aagctctagt tcaccaatat tggattctga cagttcccag
     4081 atctcactga acaaatccga cttgtcttta aaatgccagt agattgcacc gcgcgtaacg
     4141 ccagctgctt ttgcaatctc gcccagcgag gtggatgata ccccctgctg tgagaaaaga
     4201 cgtagagcca catcgaggat gtgttggcgc gtttcttgcg cttcttgttt ggtttttcgt
     4261 gccatatgtt cgtgaattta caggcgttag atttacatac atttgtgaat gtatgtacca
     4321 tagcacgacg ataatataaa cgcagcaatg ggtttattaa cttttgacca ttgaccaatt
     4381 tgaaatcgga cactcgaggt ttacatatga acaaaaacag agggtttacg cctctggcgg
     4441 tcgttctgat gctctcaggc agcttagccc taacaggatg tgacgacaaa caggcccaac
     4501 aaggtggcca gcagatgccc gccgttggcg tagtaacagt caaaactgaa cctctgcaga
     4561 tcacaaccga gcttccgggt cgcaccagtg cctaccggat cgcagaagtt cgtcctcaag
     4621 ttagcgggat tatcctgaag cgtaatttca aagaaggtag cgacatcgaa gctggtgtct
     4681 ctctctatca gattgatcct gcgacctatc aggcgacata cgacagtgcg aaaggtgatc
     4741 tggcgaaagc ccaggctgca gccaatatcg cgcaattgac ggtgaatcgt tatcagaaac
     4801 tgctcggtac tcagtacatc agtaagcaag agtacgatca ggctctggct gatgcgcaac
     4861 aggcgaatgc tgcggtaact gcggcgaaag ctgccgttga aactgcgcgg atcaatctgg
     4921 cttacaccaa agtcacctct ccgattagcg gtcgcattgg taagtcgaac gtgacggaag
     4981 gcgcattggt acagaacggt caggcgactg cgctggcaac cgtgcagcaa cttgatccga
     5041 tctacgttga tgtgacccag tccagcaacg acttcctgcg cctgaaacag gaactggcga
     5101 atggcacgct gaaacaagag aacggcaaag ccaaagtgtc gctgatcacc agtgacggca
     5161 ttaagttccc gcaggacggt acgctggaat tctctgacgt taccgttgat cagaccactg
     5221 ggtctatcac cctacgcgct atcttcccga acccggatca cactctgctg ccgggtatgt
     5281 tcgtgcgcgc acgtctggaa gaagggctta atccaaacgc tattttagtc ccgcaacagg
     5341 gcgtaacccg tacgccgcgt ggtgatgcca ccgtactggt agttggcgcg gatgacaaag
     5401 tggaaacccg tccgatcgtt gcaagccagg ctattggcga taagtggctg gtgacagaag
     5461 gtctgaaagc aggcgatcgc gtagtaataa gtgggctgca gaaagtgcgt cctggtgtcc
     5521 aggtaaaagc acaagaagtt accgctgata ataaccagca agccgcaagc ggtgctcagc
     5581 ctgaacagtc caagtcttaa cttaaacagg agccgttaag acatgcctaa tttctttatc
     5641 gatcgcccga tttttgcgtg ggtgatcgcc attatcatca tgttggcagg ggggctggcg
     5701 atcctcaaac tgccggtggc gcaatatcct acgattgcac cgccggcagt aacgatctcc
     5761 gcctcctacc ccggcgctga tgcgaaaaca gtgcaggaca cggtgacaca ggttatcgaa
     5821 cagaatatga acggtatcga taacctgatg tacatgtcct ctaacagtga ctccacgggt
     5881 accgtgcaga tcaccctgac ctttgagtct ggtactgatg cggatatcgc gcaggttcag
     5941 gtgcagaaca aactgcagct ggcgatgccg ttgctgccgc aagaagttca gcagcaaggg
     6001 gtgagcgttg agaaatcatc cagcagcttc ctgatggttg tcggcgttat caacaccgat
     6061 ggcaccatga cgcaggagga tatctctgac tacgtagcgg cgaatatgaa agatgccatc
     6121 agccgtacgt cgggcgtggg tgatgttcag ttgttcggtt cacagtacgc gatgcgtatc
     6181 tggatgaacc cgaatgagct gaacaaattc cagctaacgc cggttgatgt cattaccgcc
     6241 atcaaagcgc agaacgccca ggttgcggcg ggtcagctcg gtggtacgcc gccggtgaaa
     6301 ggccaacagc ttaacgcctc tattattgct cagacgcgtc tgacctctac tgaagagttc
     6361 ggcaaaatcc tgctgaaagt gaatcaagat ggttcccgcg tactgctgcg tgatgtggcg
     6421 aagattgagc tgggtggtga gaactacgac atcatcgcag agtttaacgg ccaaccggct
     6481 tccggtctgg ggatcaagct ggcgaccggt gcaaacgcgc tggataccgc tgcggcaatc
     6541 cgtgctgaac tggcgaagat ggaaccgttc ttcccgtcgg gtctgaaaat tgtttacccg
     6601 tacgacacca cgccgttcgt gaaaatctct attcacgaag tggttaaaac gctggtcgaa
     6661 gcgatcatcc tcgtgttcct ggttatgtat ctgttcctgc agaacttccg cgcgacgttg
     6721 attccgacca ttgccgtacc ggtggtattg ctcgggacct ttgccgtcct tgccgccttt
     6781 ggcttctcga taaacacgct aacaatgttc gggatggtgc tcgctatcgg cttgttggtg
     6841 gatgacgcta tcgttgtggt agaaaacgtg gagcgtgtta tggcggaaga aggtttgccg
     6901 ccaaaagaag ctacccgtaa gtcgatgggg cagattcagg gcgctctggt cggtatcgcg
     6961 atggtactgt cggcggtatt cgtaccgatg gccttctttg gcggttctac tggtgctatc
     7021 tatcgtcagt tctctattac cattgtttca gcaatggcgc tgtcggtact ggtagcgttg
     7081 atcctgactc cggctctttg tgccaccatg ctgaaaccga ttgccaaagg cgatcacggg
     7141 gaaggtaaaa aaggcttctt cggctggttt aaccgcatgt tcgagaagag cacgcaccac
     7201 tacaccgaca gcgtaggcgg tattctgcgc agtacggggc gttacctggt gctgtatctg
     7261 atcatcgtgg tcggcatggc ctatctgttc gtgcgtctgc caagctcctt cttgccagat
     7321 gaggaccagg gcgtatttat gaccatggtt cagctgccag caggtgcaac gcaggaacgt
     7381 acgcagaaag tgctcaatga ggtaacgcat tactatctga ccaaagaaaa gaacaacgtt
     7441 gagtcggtgt tcgccgttaa cggcttcggc tttgcgggac gtggtcagaa taccggtatt
     7501 gcgttcgttt ccttgaagga ctgggccgat cgtccgggcg aagaaaacaa agttgaagcg
     7561 attaccatgc gtgcaacacg cgctttctcg caaatcaaag atgcgatggt tttcgccttt
     7621 aacctgcccg caatcgtgga actgggtact gcaaccggct ttgactttga gctgattgac
     7681 caggctggcc ttggtcacga aaaactgact caggcgcgta accagctgct tgcagaagca
     7741 gcgaagcacc ctgatatgct gaccagcgta cgtccaaacg gtctggaaga taccccgcag
     7801 tttaagattg atatcgacca ggaaaaagcg caggcgctgg gtgtttctat caacgacatt
     7861 aacaccactc tgggcgctgc atggggcggt agctatgtga acgactttat cgaccgcggt
     7921 cgtgtgaaga aagtttacgt catgtcagaa gcgaaatacc gtatgctgcc ggatgatatc
     7981 ggcgactggt atgttcgtgc tgctgatggt cagatggtgc cgttctcggc attctcctct
     8041 tctcgttggg agtacggttc gccgcgtctg gaacgttaca atggcctgcc atccatggaa
     8101 atcttaggcc aggcggcacc gggtaaaagt accggtgaag caatggagct aatggaacaa
     8161 ctggcgagca aactgcctac cggtgttggc tatgactgga cggggatgtc ctatcaggaa
     8221 cgcctctccg gcaaccaggc accttcactg tacgcgattt cgttgattgt cgtattcctg
     8281 tgtctggcgg cgctgtacga gagctggtcg attccgttct ccgttatgct ggtcgttccg
     8341 ctgggggtta tcggtgcgtt gctggctgcc accttccgtg gcctgaccaa tgacgtttac
     8401 ttccaggtag gcctgctcac aaccattggg ttgtcggcga agaacgcgat acttatcgtc
     8461 gaattcgcca aagacttgat ggataaagaa ggtaaaggtc tgattgaagc gacgcttgat
     8521 gcagtgcgga tgcgtttacg tccgatcctg atgacctcgc tggcgtttat cctcggcgtt
     8581 atgccgctgg ttatcagtac tggtgctggt tccggcgcgc agaacgcagt aggtaccggt
     8641 gtaatgggcg ggatggtgac cgcaacggta ctggcaatct tcttcgttcc ggtattcttt
     8701 gtggtggttc gccgccgctt tagccgcaag aatgaagata tcgagcacag ccatactgtc
     8761 gatcatcatt gatacaacgt gtaatcacta aggccgcgca agcggccttt tttatgcata
     8821 acctacgaac attaaggagt aattgaacca ccaac
//
LOCUS       DAPGWD010000172         8847 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPGWD010000172 DAPGWD010000000
VERSION     DAPGWD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.173"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(440..703)
                     /gene="rpsT"
                     /locus_tag="RHX88_003819"
     CDS             complement(440..703)
                     /gene="rpsT"
                     /locus_tag="RHX88_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDV2232455.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            806..1024
                     /locus_tag="RHX88_003820"
     CDS             806..1024
                     /locus_tag="RHX88_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDV2232456.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            1032..1973
                     /gene="ribF"
                     /locus_tag="RHX88_003821"
     CDS             1032..1973
                     /gene="ribF"
                     /locus_tag="RHX88_003821"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDV2232457.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            2016..4832
                     /gene="ileS"
                     /locus_tag="RHX88_003822"
     CDS             2016..4832
                     /gene="ileS"
                     /locus_tag="RHX88_003822"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDV2232458.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4832..5326
                     /gene="lspA"
                     /locus_tag="RHX88_003823"
     CDS             4832..5326
                     /gene="lspA"
                     /locus_tag="RHX88_003823"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDV2232459.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5414..5863
                     /gene="fkpB"
                     /locus_tag="RHX88_003824"
     CDS             5414..5863
                     /gene="fkpB"
                     /locus_tag="RHX88_003824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV2232460.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5865..6815
                     /gene="ispH"
                     /locus_tag="RHX88_003825"
                     /gene_synonym="lytB"
     CDS             5865..6815
                     /gene="ispH"
                     /locus_tag="RHX88_003825"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDV2232461.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6881..7795
                     /gene="rihC"
                     /locus_tag="RHX88_003826"
     CDS             6881..7795
                     /gene="rihC"
                     /locus_tag="RHX88_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDV2232462.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7962..8783
                     /gene="dapB"
                     /locus_tag="RHX88_003827"
     CDS             7962..8783
                     /gene="dapB"
                     /locus_tag="RHX88_003827"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDV2232463.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         2048 a         2166 c         2498 g         2135 t
ORIGIN      
        1 agtgaatgtg actactatca aaaatagctc attaatttta tgaagtagaa tataaatagg
       61 tatttaacct gtgttgattg ttgttaatta tttacaagat ctaataagga gatatttatg
      121 aatggttaaa gtgtatagga atttaattca aaaatagagt ggtcttaagt ttaatacctc
      181 aatgtgtatc acaatatcca cattctttgt aggggagtct ggagattgag tagatattct
      241 tgttcagaat gtatcagccg atggttctac gattcttaag ccacgaagag ttcagaaagt
      301 gtaacggcat gtctgcaaaa gccagcatct tttgactatc tggcaaccgg tagtgtgttc
      361 tctcacgcat cataaaagca gcaggcataa aaaaacccgc ttgcgcgggc tttttcacaa
      421 agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg ttagccttat
      481 gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc acgatcggtt
      541 gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata gctgcgtata
      601 ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta cgagcctttt
      661 cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa ctcccaaatg
      721 tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc ttttgtcaat
      781 ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg atggcgcagg
      841 attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg cagcaggcgg
      901 tcactttctt cccgtgattt gcgccatggc aatgaaaagc cacttctttc tgatttcggt
      961 actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat tttcactgtt
     1021 ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc ccgcaagaag
     1081 ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc gcgctgttac
     1141 agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg ctttttgaac
     1201 ctcaaccact ggaactgttt gctaccgata aagccccggc acggctgacc cggctgcggg
     1261 aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg cgtttcgaca
     1321 ggcgtttcgc ggcgttaacc gcgcaaaatt tcatcagcga tcttctggtg aagcatttgc
     1381 gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt gaaggcgatt
     1441 tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt acgcaaactt
     1501 tttgcgaagg tggcgtgcgt atcagcagca ccgctgtgcg tcaggcgctg gcggatgaca
     1561 atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg cgtgtagtcc
     1621 acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg ctacgccgtc
     1681 aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt gaaaagccgt
     1741 tacccggcgt ggcaaacatt ggaacacgcc caacggttgc cggtattcgc cagcagctgg
     1801 aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa gtagtgctgc
     1861 gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa gcgcagattg
     1921 cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct taagcctgtt
     1981 atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa tcaaccctga
     2041 atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa ccgggaatgc
     2101 tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa aaaggcaaaa
     2161 aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat attggtcact
     2221 cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc ggttatgact
     2281 cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa gtagagcaag
     2341 aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag tgccgcgaat
     2401 acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc gtgctgggcg
     2461 actggtcgca cccgtacctg accatggact tcaaaactga agccaacatc atccgcgcgc
     2521 tgggcaaaat catcggcaac ggtcacctgc acaaaggcgc gaagccagtt cactggtgcg
     2581 ttgactgccg ttctgcgctg gcggaagcgg aagttgagta ttacgacaaa acttctccgt
     2641 ccatcgacgt tgcttttcag gcggtcgatc aggatgcact gaaagcaaaa tttgccgtaa
     2701 gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg actctgcctg
     2761 cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag atcgacggtc
     2821 aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc ggcgtgaccg
     2881 attacaccat tctcggcacg gtaaaaggtg cggagcttga gttgctgcgc tttacccatc
     2941 cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg gatgccggta
     3001 ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc ggtcagaaat
     3061 acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg ggcacttatc
     3121 cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg ctgctgcagg
     3181 aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc tgctggcgtc
     3241 acaaaacgcc gatcatcttc cgcgcgacgc cgcagtggtt cgtcagcatg gatcagaaag
     3301 gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggatcccg gactggggcc
     3361 aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc cgtcagcgca
     3421 cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg catccgcgta
     3481 ctctcgaact aatggaagaa gtggcaaaac gcgttgaagt tgacggcatc caggcgtggt
     3541 gggatcttga tgcgaaagag atcctcggcg atgaagctga tcagtacgtg aaagtgccgg
     3601 acacattgga tgtatggttt gactccggat ctacccactc ttctgttgtt gacgtgcgtc
     3661 cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag caccgtggtt
     3721 ggttcatgtc ttccctgatg atctccaccg cgatgaaagg caaagcgccg tatcgtcagg
     3781 tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa tccatcggca
     3841 acaccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg cgtctgtggg
     3901 tggcatcaac tgactacacc ggtgaaatgg ccgtttctga tgagatcctg aaacgtgctg
     3961 ccgacagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac ctgaacggtt
     4021 ttgatccggc aaaagatatg gtgaaaccgg aagagatggt ggtactggat cgctgggccg
     4081 taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca tacgatttcc
     4141 acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc ttctacctcg
     4201 acatcatcaa agaccgtcag tacaccgcca aagcggacag tgtggcgcgt cgtagctgcc
     4261 agactgcgct gtatcacatc gcagaagcgc tggtgcgctg gatggcacca atcctctcct
     4321 tcaccgctga tgaagtgtgg ggctacctgc cgggcgaacg tgaaaaatac gtctttaccg
     4381 gtgagtggta cgaaggcctg tttggtctgg cagacagtga agcgatgaac gatgcgttct
     4441 gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa gcgcgtgccg
     4501 acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa ccggaactgt
     4561 cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc tccggcgcta
     4621 ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta ctcaaagggc
     4681 tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg cactacaccc
     4741 aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc agcaacgtcg
     4801 ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt caacagggct
     4861 acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca aatacctgat
     4921 cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta atctgcatta
     4981 tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct ggcagcgttg
     5041 gttctttgcc ggtattgcga ttggtattag cgtgatcctg gcagtgatga tgtatcgctc
     5101 gaaggccacg cagaagctaa acaatatcgc ttatgcgctg attattggcg gcgcgctggg
     5161 caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact tctacgtcgg
     5221 cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg gtgcggcact
     5281 gattgtgctg gaaggttttt tgccttctaa agcgaaaaaa caataataaa ccctgccgga
     5341 taaggcctac agattgctgc gaaatcgtag gccgcatccg gcaaaaatcc tcaaaatata
     5401 agagcaaacc tgcatgtctg aatctgtaca gagcaatagc gccgtcctgg tgcacttcac
     5461 gctaaaactc gacgatggca ccaccgccga gtctacccgc aacaacggta aaccggcgct
     5521 gttccgcctg ggtgatgctt ctctttctga agggctggag caacacctgt tggggctgaa
     5581 agtgggcgat aaaaccacct tctcgttgga gccagatgcg gcgtttggcg tgccgtcacc
     5641 ggacctgatt cagtacttct cccgccgtga atttatggat gcaggcgagc cagaaattgg
     5701 cgcaatcatg ctttttaccg caatggatgg cagtgagatg cctggcgtga tccgcgaaat
     5761 taacggcgac tccattaccg ttgatttcaa ccatccgctg gccgggcaga ccgttcattt
     5821 tgatattgaa gtgctggaaa tcgatccggc actggaggcg taacatgcag atcctgttgg
     5881 ccaacccgcg tggtttttgt gccggggtag accgcgctat cagcattgtt gaaaacgcgc
     5941 tggccattta cggcgcaccg atatatgtcc gtcacgaagt ggtacataac cgctatgtgg
     6001 tcgatagctt gcgtgagcgt ggggctatct ttattgagca gattagcgaa gtaccggacg
     6061 gcgcgatcct gattttctcc gcacacggtg tttctcaggc ggtacgtaac gaagcaaaaa
     6121 gtcgcgattt gacggtattt gacgcgacct gtccgctggt gaccaaagtg catatggaag
     6181 tcgcccgcgc tagtcgccgt ggcgaagaat ctattctcat cggtcacgcc gggcacccgg
     6241 aagtggaagg cacgatgggt cagtacagca acccggaagg gggaatgtat ctggttgaat
     6301 caccagacga tgtgtggaaa ctgacggtca aaaacgaaga gaaactctcc tttatgaccc
     6361 agaccacgct gtcggtggat gacacgtctg atgtgatcga cgcgctgcgt aaacgcttcc
     6421 cgaaaattgt cggtccgcgc aaagatgaca tctgctacgc cacgactaac cgacaggaag
     6481 cggtacgtgc cctggccgaa caggcggaag ttgtgctggt ggtcggttcg aaaaactcct
     6541 ctaactccaa ccgtctggcg gagctggccc agcgtatggg caaaagcgcg tttttgattg
     6601 atgatgcgaa agatatccag gaagagtggg tgaaagaggt taaatgcgtc ggcgtgactg
     6661 cgggcgcatc ggctccggat attctggtgc agaatgtggt ggcacgtttg caacagctgg
     6721 gcggtggtga agccattccg ctggaaggac gtgaagaaaa cattgttttc gaagtgccga
     6781 aagagctgcg tgtcgatatt cgtgaagtcg attaagtcat tagcagccta agttatgcga
     6841 aaatgccggt cttgttaccg gcatttttta tggagaaaac atgcgtttac ctatcttcct
     6901 cgatactgac cccggcattg acgatgccgt cgccattgcc gccgcgattt ttgcacccga
     6961 actcgacctg caactgatga ccaccgtcgc gggtaatgtc tcggttgaga aaactacccg
     7021 caatgccctg caactgctgc atttctggaa tgcggagatt ccgctcgccc aaggggccgc
     7081 tgtgccgctg gtacgcgcac cgcgtgatgc ggcatctgtg cacggcgaat cgggaatggc
     7141 tggctacgac tttgttgagc acaaccgaaa gccgctcggg ataccggcgt ttctggcgat
     7201 tcgggatgcc ctgatgcgtg caccagagcc tgttaccctg gtggccattg gcccgttaac
     7261 caatattgcg ctgttacttt cacaatgccc ggaatgcaag ccgtatattc gccgtctggt
     7321 gatcatgggt ggttctgccg gacgcggcaa ctgtacgcca aacgccgagt ttaatatcgc
     7381 tgccgatcca gaagcggctg cctgtgtctt ccgtagcgga atcgaaatcg tcatgtgcgg
     7441 tctggatgtc accaatcagg caatattaac ccctgactat ctcgctactt tgccggagtt
     7501 gaatcgcacc ggaaaaatgc ttcatgcact gtttagccac taccgtagcg gcagtatgca
     7561 aagcggtctg cgaatgcacg atctctgcgc catcgcctgg ctggtgcgcc cggacttatt
     7621 caccctcaag ccgtgttttg tcgcggtaga aactcaaggc gaatttacct caggcacgac
     7681 ggtggttgat atcgacggtt gcctgggcaa accagccaat gtaaaggtgg cattggatct
     7741 ggatgtgaaa ggcttccagc agtgggtggc tgaggtgctg gctctggcgt cgtaacctgt
     7801 cacatgttat cggcatgcag tcattcatcg actcatgcct ttcactgata tccctccctg
     7861 tttatcatta atttctaatt atcagcgttt ttggctggcg gcgtagcgat gcgctggtta
     7921 ctctgaaaac ggtctatgca aattaacaaa agagaatagc tatgcatgat gcaaacatcc
     7981 gcgttgccat cgcgggagcc ggggggcgta tgggccgcca gttgattcag gcggcgctgg
     8041 cattagaagg cgtgcagctg ggcgcggcgc tggagcgcga aggatcttct ttactgggca
     8101 gcgacgccgg tgagctggct ggtgcaggga aaacaggcgt taccgtgcaa agcagcctcg
     8161 atgcggtaaa agatgatttt gatgtgttta tcgattttac ccgtccggaa ggtacgctga
     8221 accatctcgc tttttgtcgc cagcatggca aagggatggt gatcggcact acggggtttg
     8281 acgaagccgg taaacaggcg attcgtgacg ccgccgccga tattgcgatt gtctttgccg
     8341 ccaattttag cgttggcgtt aacgtcatgc ttaagctgct ggagaaagca gccaaagtga
     8401 tgggtgacta caccgatatc gaaattattg aagcacatca tagacataaa gttgatgcgc
     8461 cgtcaggcac cgcactggca atgggagagg cgatcgccca cgcccttgat aaagatctga
     8521 aagattgcgc ggtctacagt cgtgaaggcc acaccggtga acgtgtgcct ggcaccattg
     8581 gttttgccac cgtgcgtgca ggtgacatcg ttggcgaaca taccgcgatg tttgccgata
     8641 ttggcgagcg tctggagatc acccataagg cgtccagccg catgacattt gctaacggcg
     8701 cggtaagatc ggctttgtgg ttgagtggta aggaaagtgg tctttttgat atgcgagatg
     8761 tgcttgatct caatagtttg taaccacaaa atatttgtta tggtgcaaaa ataacacatt
     8821 taatttattg attgtaaagg gctttaa
//
LOCUS       DAPGWD010000173         8781 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPGWD010000173 DAPGWD010000000
VERSION     DAPGWD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.174"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..608)
                     /gene="upp"
                     /locus_tag="RHX88_003828"
     CDS             complement(<1..608)
                     /gene="upp"
                     /locus_tag="RHX88_003828"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDV2232464.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGD"
     gene            933..1970
                     /gene="purM"
                     /locus_tag="RHX88_003829"
     CDS             933..1970
                     /gene="purM"
                     /locus_tag="RHX88_003829"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDV2232465.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            1970..2608
                     /gene="purN"
                     /locus_tag="RHX88_003830"
     CDS             1970..2608
                     /gene="purN"
                     /locus_tag="RHX88_003830"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDV2232466.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2779..4845
                     /gene="ppk1"
                     /locus_tag="RHX88_003831"
     CDS             2779..4845
                     /gene="ppk1"
                     /locus_tag="RHX88_003831"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDV2232467.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4850..6391
                     /gene="ppx"
                     /locus_tag="RHX88_003832"
     CDS             4850..6391
                     /gene="ppx"
                     /locus_tag="RHX88_003832"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDV2232468.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6430..8673)
                     /gene="pdeF"
                     /locus_tag="RHX88_003833"
     CDS             complement(6430..8673)
                     /gene="pdeF"
                     /locus_tag="RHX88_003833"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDV2232469.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         2267 a         2169 c         2187 g         2158 t
ORIGIN      
        1 ttgtcaccgg catcgccgag gcccggaata atgtatccgt gctcgttcag tccctgatca
       61 atcgatgcgg tatacagttc gacgtccggg tgcgcttttt ccagcgcagc gataccttct
      121 ggcgcagcga ccagcaccag aactttgatg ctgctgcagc ccgctttttt cagcaggtcg
      181 atggtcgcga taacggaacc accggttgcc agcattgggt caacgatcag cgccatacgc
      241 tcatcgatgt tagaaaccag tttctggaag tacggtaccg gctccagcgt ttcttcatta
      301 cggtacatac cgacaacgct gatgcgcgcg ctcggaacgt tttccagcac accgtccatc
      361 ataccaagac ccgcacgcag aattggcaca acggtaattt tcttaccttt gatctggtcg
      421 atttctaccg ggccgttcca gccttcgata gttacttttt ccgtttcgag gtcggcggtc
      481 gcttcgtaag tcagcaggct acccacttcg gaagcgagtt cgcgaaagcg cttggtgctg
      541 atatcttgct cacgcatcag tcccagcttg tgtttgacga gtgggtgttt gacttccacg
      601 atcttcatac tctttctcct ttgaggggca gccacaaaaa aaatcgacgg attatacctc
      661 ctttcttcaa ggcggcaata ttcttttcgt tgactttagt caaaatgata acggtttgag
      721 ataaagttat tttatattca aatggttatg aaagaagatt attccatccg aaaactaacc
      781 tttaccctgg cacaagtctt ctttcgccgc gtgcctgggc aaaagacgtg caaaaaggtt
      841 gtgtaaagca gtctcgcaaa cgtttgcttt ccctgttaga attgcgccga attttatttt
      901 tctaccgcaa gtaacgcgtg gggacccaag cagtgaccga taaaacctct cttagctaca
      961 aagatgccgg tgttgatatt gacgcgggta atgctctggt tggaagaatc aaaggcgtag
     1021 tgaagaaaac gcgtcgtccg gaagtgatgg gcggtctggg cggcttcggt gcgctgtgtg
     1081 cattgccgca aaaatatcgt gaacccgtgc tggtttccgg cactgacggc gtaggtacca
     1141 agctgcgtct ggcaatggac ttaaaacgtc acgacaccat tggtattgat ctggtcgcca
     1201 tgtgcgttaa tgacctggtg gtgcaaggtg cagagccgct gtttttcctc gactattacg
     1261 caaccggaaa actggatgtt gataccgctt cagcggtgat cagcggtatc gcggaaggat
     1321 gtctgcaatc gggctgttca ctggtgggtg gcgaaacggc agaaatgccg gggatgtatc
     1381 acggtgagga ttacgatgtc gcgggtttct gcgttggcgt ggtagaaaaa tcagaaatca
     1441 tcgacggctc taaagtcagc gacggcgatg tgctgattgc actcggttcc agcggtccgc
     1501 actcgaacgg ctattcgctg gtgcgcaaaa ttcttgaagt cagcggttgt gatccgcaaa
     1561 ccaccgaact tgatggtaag ccattagccg atcatctgct ggcaccgacc cgcatttacg
     1621 tgaagtcagt gctggagttg attgaaaagg tcgatgtgca tgccattgcg cacctgaccg
     1681 gcggcggctt ctgggaaaac attccacgcg tattgccaga taatactcag gcagtgattg
     1741 atgaatcttc ctggcagtgg ccggaagtgt tcaactggct gcaaacggca ggtaacgttg
     1801 agcgccatga aatgtatcgc accttcaact gcggcgtcgg gatgattatc gccctgcctg
     1861 ctccggaagt ggacaaagcc ctcgccctgc taaacgccaa cggtgaaaac gcgtggaaaa
     1921 tcggtatcat caaagcctct gattccgaac aacgcgtggt tatcgaataa tgaatattgt
     1981 ggtgcttatt tccggcaacg gaagtaattt acaggcaatt attgacgcct gtaaaactaa
     2041 caaaattaaa ggcaccgtac gggcagtttt cagcaataag gccgacgcgt tcggccttga
     2101 acgcgcccgc caggcgggta ttgcaacgca tacgctcatc gccagcgcgt ttgacagtcg
     2161 tgaagcctat gaccgggagt tgattcatga aatcgacatg tacgcacccg atgtggtcgt
     2221 gctggctggt tttatgcgca ttctcagccc ggcgtttgtc tcccactatg ccgggcgttt
     2281 gctgaacatt cacccttctc tgctgccgaa atatcccgga ttacacaccc atcgtcaagc
     2341 gctggaaaat ggtgatgaag agcacggtac atcggtgcat ttcgtcaccg atgaactgga
     2401 cggtggcccg gttattttac aggcgaaagt cccggtattt gctggtgata cggaagatga
     2461 catcaccgcc cgcgtgcaaa cccaggaaca cgctatttat ccactggtga ttagctggtt
     2521 tgccgatggt cgtctgaaaa tgcacgaaaa cgccgcgtgg ctggatggtc aacgtctgcc
     2581 gccgcagggc tacgctgccg acgagtaatg ccccgtagtt aaagcgccag ctctgccgct
     2641 ggcgtttttc aattcacctg taaatcgcaa gctccagcag tttttttccc ccttttctgg
     2701 catagttgga catctgccaa tattgctcgc cataatatcc aggcagtgtc ccgtgaataa
     2761 aacggagtaa aagtggtaat gggtcaggaa aagctataca tcgaaaaaga gctcagttgg
     2821 ttatcgttca atgaacgcgt gcttcaggaa gcggcggaca aatctaaccc gctgattgaa
     2881 aggatgcgtt tcctggggat ctattccaat aaccttgatg agttctataa agtccgcttc
     2941 gctgaactga agcgacgcat cattattagc gaagaacaag gctccaactc tcattcccgc
     3001 catttactgg gcaaaattca gtcccgggtg ctgaaagccg atcaggaatt cgacggcctc
     3061 tacaacgagc tgctgctgga gatggcgcgt aaccagatct tcctgattaa tgaacgccag
     3121 ctctccgtca atcaacaaaa ctggcttcgt cattatttta agcagtatct gcgtcagcac
     3181 attacgccga ttttaatcaa tcctgacact gacttagtgc agttcctgaa agatgattac
     3241 acctatctgg cggtggaaat tatccgtggc gataccatcc gttacgcgct gctggagatc
     3301 ccatcagata aagtgccgcg ctttgtgaat ttaccgccag aagcaccgcg tcgacgcaag
     3361 ccgatgattc ttctggataa cattctgcgt tactgccttg atgatatttt caaaggcttc
     3421 tttgattatg acgcgctgaa tgcctattca atgaagatga cccgcgatgc cgaatacgat
     3481 ttagtgcatg agatggaagc cagcctgatg gagttgatgt cttccagtct caagcagcgt
     3541 ttaactgctg agccggtgcg ttttgtttat cagcgcgata tgcccaatgc gctggttgaa
     3601 gtgttacgcg aaaaactgac tatttcccgc tacgactcca tcgtccccgg cggtcgttat
     3661 cataatttta aagactttat taatttcccc aatgtcggca aagccaatct ggtgaacaaa
     3721 ccactgccgc gtttacgcca tatttggttt gataaagccc agttccgcaa tggttttgat
     3781 gccattcgcg aacgcgatgt gttgctctat tatccttatc acacctttga gcatgtgctg
     3841 gaactgctgc gtcaggcttc gttcgacccg agcgtactgg cgattaaaat taacatttac
     3901 cgcgtggcga aagattcacg catcatcgac tcgatgatcc acgccgcaca taacggtaag
     3961 aaagtcaccg tggtggttga gttacaggcg cgtttcgacg aagaagctaa cattcactgg
     4021 gcgaagcgcc tgaccgaagc aggcgtgcac gttatcttct ctgcgccggg gctgaaaatt
     4081 cacgccaaac tgttcctgat ttcacgaaaa gaaaacggtg aagtggtgcg ttacgcacac
     4141 atcgggaccg ggaactttaa cgaaaaaacc gcgcgtcttt atactgacta ttcattgctg
     4201 accgccgatg cgcgtatcac caacgaagta cggcgggtat ttaactttat tgaaaaccca
     4261 taccgcccgg tgacatttga ttatttaatg gtgtcaccgc aaaactctcg ccgtctgtta
     4321 tatgaaatgg tagaccgcga aatcgccaac gcgcagcaag ggctgcccag cggtatcacc
     4381 ctgaagctaa ataaccttgt cgataaaggc ctggttgatc gtctgtatgc ggcctccagc
     4441 tccggcgtac cggttaatct gctggttcgc ggaatgtgtt cgctgatccc caatctggaa
     4501 ggcattagcg acaacattcg tgccatcagt attgttgacc gttaccttga acatgaccgg
     4561 gtttatattt ttgaaaatgg cggcgataaa aaggtctacc tttcttccgc cgactggatg
     4621 acgcgcaata ttgattatcg tattgaagtg gcgacgccgc tgctcgatcc gcgcctgaag
     4681 cagcgggtgc tggacatcat cgacatattg ttcagcgata cggtcaaagc acgttatatc
     4741 gataaagaac tcagtaatcg ctacgttccc cgcggcaatc gccgcaaagt acgggcgcag
     4801 ttggcgattt acgactacat caaatcactc gaacaacctg aataacccta tgccaataca
     4861 cgataaatcc cctcgtccgc aggagtttgc tgcggtcgat cttggttcaa acagttttca
     4921 catggtcata gcccgtgtgg tagatggtgc catgcagatt attggccgcc tgaaacagcg
     4981 ggtgcatctg gcggacggcc tggggccaga taatatgttg agtgaagagg caatgacgcg
     5041 cggtttgaac tgtctgtcgc tgtttgccga acggctacaa gggttttctc ctgccagcgt
     5101 ctgtatagtc ggcacccata cgctgcgtca ggcgctgaac gccactgact ttctgaaacg
     5161 cgcggaaaag gtcattccct acccgattga aattatttcc ggtaatgaag aagcccgtct
     5221 gatttttatg ggcgtggaac atacccaacc ggagaaaggt cgcaaactgg ttattgatat
     5281 tggcggcgga tctacggaac tggtgattgg tgaaaatttc gaaccgattc tcgttgaaag
     5341 ccgccggatg ggttgtgtca gctttgccca gctttacttc cctggcgggg tcatcaataa
     5401 agagaatttt cagcgcgctc gtatggcggc agcacaaaaa ctggaaactt taacctggca
     5461 attccgtatt cagggctgga acgtggcaat gggcgcttcc ggtaccataa aagccgccca
     5521 tgaagtgtta atggaaatgg gcgagaaaga cgggataatt accccggaac gtctggaaaa
     5581 actggtaaaa gaagttttac gtcaccgtaa tttcgcatcg ctgagtttac cgggtctttc
     5641 cgaagagcgg aaaacagtct tcgttcctgg actggcgatt ttatgcggtg tgtttgatgc
     5701 tttagccatc cgtgaactgc gcctttctga cggggcgtta cgcgaaggcg tactgtacga
     5761 aatggaagga cgtttccgtc atcaggatgt gcgtagtcgc accgccagca gcctcgccaa
     5821 ccagtatcac atcgacagcg aacaggcccg acgggtgctg gataccacta tgcaaatgta
     5881 cgaacagtgg cgggaacagc aaccgaagct ggcgcatccg caactggagg cgctactgcg
     5941 atgggccgcc atgctgcatg aggtcgggtt gaatatcaac cacagcggtt tgcatcgcca
     6001 ctccgcttat attctgcaaa acagtgactt gccgggtttt aatcaggaac agcagctgat
     6061 gatggcgaca ctggtgcgct atcaccgtaa agcgattaag ctcgacgatc tgccgcgctt
     6121 taccttgttt aagaagaaac agttcctgcc actgatacag ctattgcgcc ttggcgtatt
     6181 actaaacaat caacgtcagg caaccaccac accgccaaca ttgacactga ttactgatga
     6241 cagtcactgg acactgcgtt tcccgcatga ctggtttagt cagaatgcgc tggtactgct
     6301 tgatctggaa aaggagcaag aatactggga aggcgtggct ggctggcggt tgaaaattga
     6361 agaagaaagt acacctgaaa tcgccgctta atgctttgcg ggccgacgag aaatgtcggc
     6421 ccgcattatt caggcacttt cgcgaatggg ttcgatttca ttcaacgtat caattaacgg
     6481 ctgcggctta ccaataagat aaccctgcat ataatcgatc cccaaagaga gcaccgcctc
     6541 gcggatctct tcgttttcaa cgtactctgc cactacccgc attttcttca ttcgcgccag
     6601 gtggcaaatc gatgccacta tctgataatc cagactattt gacacaatat tgcggataaa
     6661 actgccgtca attttaagca gatcggcatt cacattttta agccgtgcat agctggcgta
     6721 gccggtgccg aaatcatcaa tcgcaatctg gcagcctaat tcctgaagat gctgcaaggt
     6781 gatttgcgcc tgcttaacat tggtcagagc attactttcg gtgacttcaa aaataagttg
     6841 ccacgcttca atctgatatt tagccagcaa ctgactgact tcaacaggaa aacgagcctg
     6901 acataccgag gttggagaca gattaatagc aaaacggtga gcgggcatct tcgctctgtt
     6961 ttcagccata aattgcagcg tatgctcaat gacccacatg tcgatactcg acgataaacc
     7021 aaattcgtgc gcgaccggca agaagctatc ggggctgatc agttcatcat tctcaccttt
     7081 catgcgcaga agaatttcat ggtaaacatc accacgcata ccggtaatcg gctgggccat
     7141 caggaaaaaa tggttgtgtt ccagcgcctg ctgtagtcga ttcatcatcg cgactttatc
     7201 tttcaattcg cgttgcaaat acattgcccc gcgacgctgc atattttccg gggcgttggt
     7261 cacgatggaa agttcggcga ccgtatttag ctctcccagc agcaggtaga tatgattcac
     7321 tggcgagcgc acatagcagt aactgacgcc aatctgcggt tgcatcggca tgccatccca
     7381 aaagaaacga aattgcttga gatggctatc cagtgcggta atgcgctcct ggtgcgattc
     7441 tgtattcagc cgcagcgcga gatcgttacc cgaaagctga taaacatctt cacccggttc
     7501 cagcaagggt gacagccagt gagaaagttt ttgcttgtat tgaatccgca gcatgatgcc
     7561 atagttctta accagcattt ccatgccagg gatgcgtaaa taacaaagcg cagaccaggg
     7621 ggcatcacgc aacgcgcgat tcagggcgcg aacatttggc agatgaacca ccgggtccac
     7681 atacgcaagc cgctgaatgc gtctgactac cgctcgctga cgagttgcca gtacagccat
     7741 ataattgaca ataaaagaga ataccagata actggaggag gttatggtca gctgcgtggt
     7801 atagccagga taaatgggaa tgtaattttg atagctgtgg atgctgatca tcaacacgac
     7861 cgcccagagc agcgaaatca gcttataacc atagcgcatc gctccccaca tcatcagggg
     7921 cagcaataat gacaaggtat aattggtgct aaaaattgtg cttttttcat ttaacggcat
     7981 gcataacagc aacaataaag cacctaatgc cagtagccag agcgcgaact cttttttggt
     8041 gactttggca tcaacctgct gttttaattg cgaataatag ctacgtaaat aaaatggatt
     8101 tcgcactacc cgaatgatga agtagcacag cgggacaccg atcagattac ccaccagtaa
     8161 ggcctgataa ttgattaaag tcccgaggtt aaaaggcatg acaccgacca gattttctct
     8221 gctcgccagc aatcctacaa atgcagcaaa ctggaaaagt atcagaaaca gcgttgcagg
     8281 aaacactatc tgccagaata tacgctggga aattaaacgg gtatcaccat gtgaaacgtt
     8341 gttacgccgg ggagtaaaga ccctgtaacc gccccaacag agaatgataa tgataataaa
     8401 attagccgtt aatgagagcg tttcgtaaaa acccagctct gcatatttgc ggacaaaaat
     8461 ccctaaggtg atccctggca atgccgccca gctgaaaaac atcatcatgc tgatcatcag
     8521 tgccaaaggc agataaaaga gaaaaacctc accggaagaa atatgcgcga aagtattaat
     8581 gtgggcgaaa atgggcaaga ttaaagaagg caggaacagc ggaagccccc accatttatc
     8641 acgtattttt atataagttg cattcagttt catagatgct cagcagaatc ccccacatcc
     8701 tgaaggaggt gtattcagac aggcatccca cctgacttcg aatgatgatt attcatcact
     8761 atagagagca ttgattctaa g
//
LOCUS       DAPGWD010000174         8540 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPGWD010000174 DAPGWD010000000
VERSION     DAPGWD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.175"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(67..945)
                     /gene="glxR"
                     /locus_tag="RHX88_003834"
     CDS             complement(67..945)
                     /gene="glxR"
                     /locus_tag="RHX88_003834"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV2232470.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1045..1821)
                     /gene="hyi"
                     /locus_tag="RHX88_003835"
     CDS             complement(1045..1821)
                     /gene="hyi"
                     /locus_tag="RHX88_003835"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDV2232471.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1834..3615)
                     /gene="gcl"
                     /locus_tag="RHX88_003836"
     CDS             complement(1834..3615)
                     /gene="gcl"
                     /locus_tag="RHX88_003836"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDV2232472.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3705..4520)
                     /gene="allR"
                     /locus_tag="RHX88_003837"
     CDS             complement(3705..4520)
                     /gene="allR"
                     /locus_tag="RHX88_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDV2232473.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCL
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4598..5080)
                     /gene="allA"
                     /locus_tag="RHX88_003838"
     CDS             complement(4598..5080)
                     /gene="allA"
                     /locus_tag="RHX88_003838"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDV2232474.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5310..6236
                     /gene="allS"
                     /locus_tag="RHX88_003839"
     CDS             5310..6236
                     /gene="allS"
                     /locus_tag="RHX88_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDV2232475.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6305..7399
                     /gene="mnmH"
                     /locus_tag="RHX88_003840"
     CDS             6305..7399
                     /gene="mnmH"
                     /locus_tag="RHX88_003840"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDV2232476.1"
                     /translation="MQERQTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            7515..7879
                     /locus_tag="RHX88_003841"
                     /pseudo
     CDS             7515..7879
                     /locus_tag="RHX88_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2176 a         2233 c         2023 g         2108 t
ORIGIN      
        1 gctgctttct tttttaatat tgctggccga ttcattttct gatatcggcc atttttattg
       61 cgggtatcag gccagtttat ggttagccat taattccagc gcctgcacta acgcagagtg
      121 atccaactgg ctgccaccgt ttgccgcaca ggtattaaat aactcctggc aggtcgcagt
      181 gtttggcagg ttcagcgcaa gtgctttcgc actttgcagt gccaggttga gatctttctg
      241 gtgcagagcg attttgaagc ccggattaaa ggtgcgttta atcatacgct cgccatgaac
      301 ttccagaata cgtgaggaag caaagccgcc catcagcgcc tggcgcacac gtaccgggtc
      361 cgcaccggct tttgaagcaa atagcagggc ttcagaaacc gcttcaatat tgagcgccac
      421 aataatctga tttgccactt tgcaggtttg accgtcgccg ttaccgccca cgagggtgat
      481 atttttaccg agcagttcaa acagcggttt aacacgttca aataccgctt catcaccgcc
      541 aaccataatc gacaacgtcc cttcacgcgc accgatttca ccgccggaga ctggcgcatc
      601 gagataatcg ccgcccagtt cattcacctg acgagcgaaa cgcttagttt caatcgggga
      661 aatggagctc atatcaacaa tggttttgcc cttcagcgag gctttggtac aaccattttc
      721 accgaacaga acttcttcaa cctgaggtgt gtccggcacc ataataaaaa tgatgtccga
      781 tgcttccgtt acctggcgag cagtttcaac actgacggca cccagtgaca gtaattcatc
      841 agcaaccggt ccaatggtcg tgacatgtaa ttgatgaccg gcacgcgcca gattaatggc
      901 catcggtgta cccataatgc ctaagccaat aaatcccagt ttcataatta acctctttta
      961 aatttcgctt ttcctgaatt cagacaacac gatcccctgc caacttagcg gcctaaaaaa
     1021 gcattgtctg aatagcgtta cgttttaacg gtacggatcc atccagcgta aaccggcttc
     1081 cgtggtggtt tggggtttat attcacaccc aacccaaccg ttgtagtcag aattttcgat
     1141 taccttaaag agataatcat aattaatttc tccggttccc ggttcgccgc gatgcggatt
     1201 atcggcaatt tgcaggtgac caattttatc agcccactga gtcatggtgt tggttaattc
     1261 accttccatc cgctgcatgt gataaatgtc atactgaatt ttcaaattgc agcaaccaac
     1321 atcatcaatc aatttcagcg cctgccgagt tccggtgaga tggaaaccag gaatatcaaa
     1381 atggttaata ggttcaatca gtaataaaat atcttctttc atcagcatat tcgcggcata
     1441 acgcaggttt tctacaagcg ttgcgtgaat ctgttcactg ctgaaaccag ccggcgtttt
     1501 accgaccaga cagttaattt ttttattacc cagcgcacgg gcataacgaa tcgctgctgc
     1561 tacgccatcc cgaaactctt cttcacggcc aggaatacag gcaataccgc gctcccccgc
     1621 cgcccagtca cccgccggta aattgtgcag cgtatgttcg agtttattac tcgccagcac
     1681 ctgttttaat tcttcaatgt cgtagtcata aggaaacata aattcaacgc cgcgaaaacc
     1741 acactgcgca gctttctcaa aacgggcgag aaaatcatat tctccaaata acatcgataa
     1801 attagcagag aaacgtaaca ttatttatct cccttattca tagtgcatga agcaggtttc
     1861 agtcggtgcg tccgctgcgt tatcggcgat atcttcaaat tccatgacgt tatccagttc
     1921 gctgcccatc gaaatattgg tcacacgctc gagaataact tccacgacta ccggtacccg
     1981 atattgcgcc attaaggctt tcgcctgttc aaaggctggc gcaatatctt ccggtttgaa
     2041 gacgcgaatc gctttacaac ctaaaccttc cgctactttt acgtggtcaa caccgtagcc
     2101 attcacttca ctggagttga tattctcgaa agcgagttgc acgcagtagt ccatatcaaa
     2161 cgcgcgctgc gactggcgaa tcagccccag ataagcgtta ttgaccagta catggatgta
     2221 cggaatgttg aactgtgcgc caacggccaa ttcttcaatc aggaactgga agtcgaagtc
     2281 accggaaatc gccaccactt tgcgctctgg atcagcggca caaaccccca gcgccgccgg
     2341 aatggtccag cccagcgggc ctgcctgccc acagttgatc cagtggcggt ctttaaagac
     2401 atgcagcatt tgcgcagcgg caatttgcga cagaccaatg gtggtgacat agcaaacatc
     2461 gcgaccaaag gctttgttca tctcttcata cacgcgctgc ggtttcaccg gcacgttgtc
     2521 gaaatgggtt ttgcgcagca aagtgcgttt gcgctgctgg cagtcggcga cccattcttt
     2581 acgacacggc agacgacccg ctttttgcat ctcctgcgcc acttcaacca gcagtgtcag
     2641 cgccgcttta gcatcagaga caatgccgag atccggacac agcacgcgac caatttgcgt
     2701 cggctcaata tcgatatgaa cgattttgcg cccttcggtg tatttctcta ccgaaccggt
     2761 atgacggtta gcaaaacggt taccgatacc aaacaccatg tcggacgcca gcaacgttgc
     2821 gttaccgtaa cgatgcgcgg tttgcagacc caccatcccg gccatcagtt catgatcgtc
     2881 cgggatacag ccccagccca tcagcgttgg gatcaccgga acgctggtca gttcagcaaa
     2941 ctgttgtaac agtgcagctg cgtcagcatt aattaccccg cccccggcaa caatcaccgg
     3001 acgttcggcc tggattaaca tttctacagc tttttcgatc tgcatacggc tggcagcagg
     3061 tttgtagacc ggcagcggtt cgtacatatc aggatcaaac tcgatttccg caacctgaac
     3121 gtcgaacggt aaatccacca gtaccggacc cggacgacca gaacgcatca ggtgaaatgc
     3181 ctgttgcaac acacgaggca ccagcgccgc ttcacgtact gtaaccgcca ttttacttac
     3241 cggcttggcg atagcttcga tatcaacagc ctggaaatct tctttatgca gacgggcgcg
     3301 cggagcctgg ccggtaatac acagaatagg aatggaatca gcagaagcag aatagagcgc
     3361 ggtgatcatg tccgtgcccg caggaccgga agtccccaga catacgccga tattccctgc
     3421 cgttgcgcgg gtataacctt ccgccatgtg cgaagcacct tccacatgac gcgccagaat
     3481 gtgacgaata ccgccgtgct tacgcatcgc tgagtagaac ggatttattg cagctcccgg
     3541 aacgccgaag gcggtggtga taccttcttt ctccagcaca tacattgccg cgtcaacggc
     3601 tctcattttt gccattttat tcctacctct attaattgga aaatttttcc aactttcaaa
     3661 ttttgtcaaa aaaacccgct ccgcagagcg ggatgcgaca gacattatgg atgtgctttc
     3721 agtcccaacg ccgtgctgat atcgcgggcg gtgtctctga ccagctcacc ctgactgaca
     3781 aaacgatctt ctgtcagtct tgatgaaggc ccggagatgg agatagcggc aacaacacta
     3841 cctacatcat cgtaaattgc tgaagctaag caattcagac ctacaacatg ctcttcttta
     3901 tctacggtat agcccagttc acgcgcttgt tccaggtcct tcagcaaggt gggcatatcc
     3961 acaagcgtag ttggcgtaaa ctgctgcaaa ccggtttgca gaatgatgct catcaactcc
     4021 tcttccgcca gcggataaag cagcgctttg cccgcaccgg aagcatgcag cggcagacga
     4081 ctgcccagtg gcgcacacat cctgaccatc gatttacact ctaactgacc aattaatacc
     4141 gcttcattgc cgttacggat cgcgacattg accgtttcgc cggaaagtaa catcaggcgg
     4201 cgcataaacg gcccggcgac ggagaggaca tcgcggttat ggatgtacgc cgcaccgacg
     4261 ttaaagacac ctaatcctat atgccaccag cctaattgac tgtcctgata gacaaaatcc
     4321 gctgcctgta aaaccttcag caagcgaaag gtcgtggaga gcggcaaatc cagattgaga
     4381 gaaatatcgc taaccgacga acttccccca cttttttcca aatattgcag aatcgcaatt
     4441 ccccgctcta acgcctgtgc gcccttctgt gccacaggct ccgcctgtcc tggcctgccg
     4501 cgccgtctaa cttccgtcat acctacgtta actccgacct acaagtgagc ataaacaatg
     4561 aacaattagt ttatcttatg caagtcggtt gcaggcgtca cgcaaaacag agttcctgtt
     4621 caggaatact ttcaacatca cagttgtcac tgccgccgcg atcgatggtc agaaaatcgg
     4681 tgacgcgctg ccaggcgaaa agtgggtgat gccagacgtt acgatggtaa ttcaccccct
     4741 gttcaccgtt ggtgataaac gcccgcagcg ttgacaggtc tggtttgtcg tcgcctaacg
     4801 ccacgaccac cacaaacact tcacctttca ttgggataaa ggcctgagtg cccagcggat
     4861 gacgttcgag ttcgtgaatg gtcagcggca gattcgccgg ttgcgcgcgg ttaatgctga
     4921 taagcgtacg gtcttgctca agaatctcaa ccagcgccaa atcgtggtaa cgctccacca
     4981 ggccattgtt aatatggaaa aaatcccgtt gctgcgtttc gattacgtcg ccataagcac
     5041 taaaggcttc ctgacttaac ggtaatacct gaagtttcat ctttttgtcc ttgtaacata
     5101 attccgttca accaccgccg accggtcagc ttggtttcaa taacaggcaa tctaaaacct
     5161 taagagaaca acatcaatac aaaagttgga atatttttcc aaaactagat ttagtccaca
     5221 aattgaacag ggttatcatg taaaaatact gataaatcag aggataagaa aatcacgcaa
     5281 cttgcggatt taaaagggga tacctgaaga tgttcgatcc agaaaccttg cggactttca
     5341 ttgcggttgc tgaaacagga agtttttcaa aagcggcaga acgattatgt aaaaccacgg
     5401 cgacgatcag ttatcgcatt aaacttctgg aagagaatac cggagtagcg ctgtttttcc
     5461 gcacgactcg cagcgtgacg ttgacagcgg ctggcgagca tctactttcc caggccagag
     5521 actggctgag ctggctggaa agtatgccaa gcgagctgca acaggtgaat gatggcgtgg
     5581 aacgccaggt gaatattgtc atcaacaacc tgctctacaa cccccaggcc gtcgcccagt
     5641 tgctggcgtg gctgaatgaa cgttacccct ttacccagtt tcacatctcc cgacaaatct
     5701 atatgggcgt ctgggattcg ctattatacg aaggtttttc gctggctatc ggcgtcacgg
     5761 gaactgaggc gctggcaaat acctttagtc ttgatccctt aggatcggtg caatggcgat
     5821 ttgtcatggc ggcggatcat ccgctggcga acgttgaaga gccgctaaca gaagcgcagt
     5881 tgcgtcgctt tccggcggtc aatattgaag acagcgcccg caccttaacc aaacgcgtcg
     5941 cctggcgatt gccagggcaa aaagagatta ttgttcctga tatggaaacg aaaatcgccg
     6001 cccatctggc gggcgttggc attggttttt tgccaaaatc gctttgccag tcaatgatcg
     6061 ataatcaaca actggtcagc cgggtaatcc caacgatgcg ccctccttcg ccattgagtc
     6121 tggcatggcg caaatttggc agcggcaaag cggtagaaga tattgtgacc ttgtttaccc
     6181 agcgcaggcc ggaaatcagc ggatttttag aaattttcgg caacccacgc agttaaaaat
     6241 caagtttcct tgcgcgttgg cgcgtaaaat agcgcgcaag ctgatttgag gttcactttt
     6301 aactatgcaa gagagacaaa cggaacagga ctatcgtgcc ctgctgattg ctgatacgcc
     6361 cattattgat gttcgcgccc ctatcgagtt tgagcaaggc gcaatgcccg ccgctatcaa
     6421 tctgccgtta atgaataacg atgaacgcgc cgccgttggc acctgctata aacagcaagg
     6481 ctcagacgca gcgctggcgc tggggcataa actggtggcg ggtgaaattc gtcagcagcg
     6541 catggacgcc tggcgggcag cgtgcctgca aaatccgcaa ggtattctct gctgcgcccg
     6601 tggcggtcag cgctcacata ttgtgcaaag ctggttgtat gcagcgggga ttgattatcc
     6661 gctggtggaa ggcggttata aggcactgcg ccagaccgcg attcaggcga ctattgaact
     6721 ggcacaaaaa ccgatagtgc tgattggcgg ttgtactggc agcggtaaaa cgctgttagt
     6781 gcagcaacag ccgaacggtg ttgatctgga agggctggcg cgccatcgcg gttcggcgtt
     6841 tggtcgcacg ttacaaccac aacttagcca ggcgagtttt gaaaacctgc tggctgccga
     6901 aatgctgaaa accgacgccc gtcaggattt gcgcctgtgg gtgctggaag acgaaagccg
     6961 gatgatcggt tcgaatcacc tgccggaatg cctgcgcgag cgaatgactc aggcggcgat
     7021 tgcggtggta gaagatccgt ttgagatccg tcttgagcgc ctgaacgaag agtatttctt
     7081 gcgtatgcat catgatttta cccacgctta tggcgacgaa cagggctggc aggaatattg
     7141 cgaatacctg catcacggac tttcggcgat taagcgtcgg ctggggttac agcgctataa
     7201 cgaactcgct gcccagctgg atacggcgct gacaacgcaa ctcaccaccg gcagcaccga
     7261 cggtcatctg gcctggctgg tgccgttact taaagaatat tacgacccaa tgtatcgcta
     7321 tcagcttgag aaaaaagcgg aaaaagttgt ctttcgcggt gagtgggcgg aagtggcgga
     7381 atgggttaag gcgcggtaat gagggacgta tttgtattag tcgcgacttg atctgacaca
     7441 tggccttgaa aggttgagag ttaccggttt tgatatgagt gtcgaatcct tatccaaaac
     7501 aagaggtaac tctcatgctt catactgcca atcccgtcat caaacacaaa gccggtttgc
     7561 tcaatctggc tgaagaactc agcaacgtat caaaagcctg taaaatcatg ggcgtttcgc
     7621 gtgatacgtt ttatcgttac cgcgaactgg tcgctgaagg cggtgtggat gcgcagatta
     7681 atcgtagtcg ccgcgctcct aaccttaaga accgtaccga tgaggcaact gaacaggctg
     7741 ttgttgatta cgccgtcgct ttcccgacac atggtcagca ccgggccagc aacgagctgc
     7801 gtaaacaggg ggttttatct ccgacagtgg tgtccgttct gtctggttac tccataacct
     7861 tgagaatctc aaaagacgat actgaacaaa ttttatcagg tgacgttccg taaaaagttg
     7921 tatggagttc tcgatgcagt cggcgcgact atcagtaccc ccgctgaacc ttggactatc
     7981 gggccgccgt actggggcat gtcaccctgg cgtgccgctg gttttccgct aatggtttat
     8041 ttcctctttc tttcccggca agaatttatt ttaatccgga aggattattt attttcagat
     8101 agggactcac attaaacacg taacatttta attaatgcat ggctgaatat gtattacaca
     8161 atgtcagata tcgtaaaaga ccagtgcaat tctatgtaaa ctctctgact gttcatttta
     8221 tttgttgttt cagggtcgga actcaccctc aataaacaac tgaatacgag tagatattca
     8281 tcgtctgagc tatatggctt tacacaatag ccgacatgtc ttttctcagt caggaatttt
     8341 acttttcctc actacgaagt tgacgacttc gatatgggat agactcttaa ttcaagcaat
     8401 tactctgcac aagcatcagg caatccagct ccaatatttg ttataaaata atgcgtaaca
     8461 ttaatttcac caacaaaaaa aaggtttacg ctatcccctt gccctggtat tttaccatat
     8521 ttacaccaca ctttttttgt
//
LOCUS       DAPGWD010000175         8525 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPGWD010000175 DAPGWD010000000
VERSION     DAPGWD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.176"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(212..424)
                     /locus_tag="RHX88_003842"
     CDS             complement(212..424)
                     /locus_tag="RHX88_003842"
                     /inference="COORDINATES: protein motif:HMM:NF014080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232477.1"
                     /translation="MHSSNQQAAIQHLLENGEATFRELADALGVTNKKIQNLLQNIQR
                     YGQLVREGKKYRLVPNWQEKSALQKR"
     gene            complement(427..1392)
                     /locus_tag="RHX88_003843"
     CDS             complement(427..1392)
                     /locus_tag="RHX88_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232478.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDDGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQSDPQTAEQNYLTQYPDAVVFSAKKRQWGNQEDLACAQWIWAR
                     IVSLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHKQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDSEIPPGFRG"
     gene            complement(1373..1894)
                     /locus_tag="RHX88_003844"
     CDS             complement(1373..1894)
                     /locus_tag="RHX88_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232479.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(1878..2105)
                     /locus_tag="RHX88_003845"
     CDS             complement(1878..2105)
                     /locus_tag="RHX88_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2232480.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            2183..2590
                     /locus_tag="RHX88_003846"
     CDS             2183..2590
                     /locus_tag="RHX88_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2232481.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            2783..2935
                     /locus_tag="RHX88_003847"
     CDS             2783..2935
                     /locus_tag="RHX88_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDV2232482.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            2935..3312
                     /locus_tag="RHX88_003848"
     CDS             2935..3312
                     /locus_tag="RHX88_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232483.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPGVLAGEICTEISSELLPVIREAICRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
     gene            complement(3281..3481)
                     /locus_tag="RHX88_003849"
     CDS             complement(3281..3481)
                     /locus_tag="RHX88_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232484.1"
                     /translation="MTKEEFVSYIFDKTVEMYAATYGSCNPLNKPEGKDDFDKIYRFL
                     EDRYIKRLEDAGIKSPVKSPLS"
     gene            3984..4172
                     /gene="dicB"
                     /locus_tag="RHX88_003850"
                     /gene_synonym="ftsT"
     CDS             3984..4172
                     /gene="dicB"
                     /locus_tag="RHX88_003850"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDV2232485.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            4169..4360
                     /gene="ydfD"
                     /locus_tag="RHX88_003851"
     CDS             4169..4360
                     /gene="ydfD"
                     /locus_tag="RHX88_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HDV2232486.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            4454..6925
                     /locus_tag="RHX88_003852"
     CDS             4454..6925
                     /locus_tag="RHX88_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDV2232487.1"
                     /translation="MSKVFICAAIPDKLATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYTAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETKSDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLART
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKEPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPKEMEGAEHPHKGNTGGNQHHDSDNETGETADHS
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            6998..7249
                     /locus_tag="RHX88_003853"
     CDS             6998..7249
                     /locus_tag="RHX88_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDV2232488.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            7284..>8525
                     /locus_tag="RHX88_003854"
     CDS             7284..>8525
                     /locus_tag="RHX88_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2232489.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQLALLNTQLSEF"
BASE COUNT         2441 a         1991 c         1948 g         2145 t
ORIGIN      
        1 attataaaaa cattttatta acaattccac taaattgaaa cataatgaca acatgaaaca
       61 aaacgttaga ggtcagttgg catcaattgc tctacctaag ctctgttacc gactccccga
      121 ctactcccca aaattttttg tctctgatat ccgttgctgt tccggcgaca ttcctgcgcc
      181 gcagcctgtt tcgacgctgt tgacctttcg gttaccgctt ttgcaatgca gatttttcct
      241 gccagttcgg taccagccga tattttttcc cctcccgcac aagctgcccg taccgctgta
      301 tgttctggag caggttttga attttcttgt tggtcacacc aagagcatca gccagctccc
      361 ggaacgtggc ttccccgttc tccagcaaat gctggattgc ggcctgttga ttactgctgt
      421 gcatacctac cccctgaatc ccggtggaat ttcgctgtcc ggctcagaaa tatgattcac
      481 acaacgctgt acaggtgaac gccccaggcg aatgaccagt tcatcccatt tttcgcggag
      541 ctttgaaggg ctcatgatgt tttttaccca gaatggatct cgctgaaccc gaccaaacat
      601 ttcgcaaatt tgtttatggc ttctgccatc cagcatccgc attgtgcgca cgtcattagc
      661 ccaggcagtc cagttaggct cttttggtcg catgatctcg ccatcatcac tggcggcctg
      721 ttcgtaaagg ctcacgattc gtgcccagat ccattgcgca cacgccaaat cctcctgatt
      781 cccccactga cgttttttag cactgaaaac caccgcgtca ggatattggg ttaaataatt
      841 ctgttcagct gtctgcgggt ccgattgcga agcttccgga cgaaaagtgt ttttattctc
      901 tgtagtaatc tctgttgtat tctctgtaag atcatcaggc cattttgacc cgatgacatt
      961 gggtcgtttt gaaccaatgg agcgtttcat tttgacctct tccatcgtgt cattttgacc
     1021 tgatggagcg gcgcattttg aaccgatgga ttcgctcaat ttgccatcat ctaaaagctc
     1081 gctcccatag ttaatcgtgt agaaattggt catatcgcgc tttgatttat tgagcttttc
     1141 acaacgcaaa agccccagcg ttttcagact tgcaaacgcg cgctttaacg ttgactctga
     1201 ccagaatggg aactgttcca gccattgttc cgttgtgttg taaatccagc gaacaccatc
     1261 acattccatg ccggaattgg tatctctcaa ccagtaatgc aactgctgca acacaatggc
     1321 ttcatttaag ccaatcttca tcgcaagctg tgtgtttata accagtgggc gttcagcaaa
     1381 aagaaggctc ataattccat ccagcttttt gttggtattg ctgtcgatac gcaagtttga
     1441 aagcaattgc tttttctata agttcgtcag tttcacgatc cactacggca ggatcagcaa
     1501 aaagcagtcc ggactccacc acatcgccat attctttgtt taacccggcg atcatgtacg
     1561 tgatgctttt tccgtcacta atttcacgat acaacctgaa atcattaatc cggatagcct
     1621 ccataattgc aggcactagc gccgtgaact tttcacgctt atccctggtg tcgatagcct
     1681 tccagcgttc gaatatcttc actcgattaa cgccaagcgc tcgctgatca accgcgccac
     1741 cttcatctgt gacacgctga acatcgatgt tcgggcgctc tttcaaagcc cagaatgctt
     1801 cagtgattaa tatcgtcgcc tgctcctgtg tcattcctgg tcgacatatc caagcatcca
     1861 gagcctcacg agcctgttca ggagtgattt tcattgttca accgccccgc ccgcttcgtc
     1921 ttacgatatt cgtcataaac cttgggatca tacagaagct cgccgccaga tgcctcctgt
     1981 agacgcatcg cgcgaccttc aggaaccagc tcttgccatt gagaaacagc agatgggtca
     2041 acgccagcag ctttcgctac tttggctttc gtaccgtaaa aattaattac gtctgattta
     2101 aacatcgcac ctccaatatt gagttttctc aatgctaatc actcaaggaa tctcaagtca
     2161 agggttatta agatatctaa atatgaacga gaaaacttta ggtcaacgca ttagagaaag
     2221 acgtaagcag gtaggcttaa gtcaaaacgg tttaagcaaa gctgctggcg tatctggctc
     2281 atcaatttca ttatgggaaa gtgaccatac agccccgcgc gggcaaaatt tgcatcgcct
     2341 cgccgaggta ttgcaatgtt caccaacctg gatactgttt ggcgacgagg acaaaacacc
     2401 agctccccct gtttcactcg atagtgcctt agacttatcg gaagatgagt tagagatgct
     2461 gcgtttgtat cgcgcacttc ctaaatcaga gcaacaagca caactcagcg aactccgcgc
     2521 ccgcgttgag aattttaatc gcctgttcac cgagctatta gaggctcgca aacgcaacaa
     2581 gcatcaataa tcatcccttc ataaaatttt aaagccttac atttcaatgt attggcttta
     2641 tcttgcgcca atacttgagt tttctcatca aaaaaccttg acgaaaaata atgagaaaac
     2701 taaattacct ccatcaacac accgcacggt gttctcagca aacagttccg ctaccccggc
     2761 gttaagggga aatgaggtca gcatggatac tatcgatctt ggcaacagcg aatctctggt
     2821 atgtggcgtg ttccccaacc aggacggtac gttcaccgcg atgacatata ccaaaagcaa
     2881 aacgtttaaa accgaatctg gcgcgcgttg ctggttagcc agaaacactg actgatgagg
     2941 ctgacgatgg aatttaaaga tttaccgtct gatgtacaga aaacagcagc tcatacattg
     3001 cattctgtgc tgcgagaaat cgggaaagat attgcaagcg agccagcaaa agatctggcc
     3061 cggaaaatca agaccgcttt cgttgagctt tataatgttg gcactgactc tgaaactgtc
     3121 gagaccaaga ccgtaagttc accaatattc tcacttgagc caggcgtatt agcaggtgaa
     3181 atatgcaccg agatttccag cgaactgctt ccggtaatac gagaagcaat ttgccgtcgc
     3241 ggtttggatg gaagttacga tcatgacgtc ctgcaagttc tcaggacaat ggtgacttca
     3301 ctggggattt gatccctgcg tcctctaacc ttttgatata gcggtcctcc aagaagcggt
     3361 aaattttgtc gaaatcatct ttcccctctg gtttattcag aggattacag gacccgtaag
     3421 tagcggcata catttcaacc gttttatcaa aaatataaga gacaaattct tctttagtca
     3481 tatagatttc cttcttggtt attcgggata agaaggatac cacctcgcct gacgtggtta
     3541 aaagcaggca cacaacacga aagcgtacgg cgaagttcgt ctcactgtac ggtgtcgtta
     3601 aatttaattc gaccgtgcgc ttccggttgt ggcaacccgc gaaatggcgc ggcggtaagt
     3661 atggcggggt tattccttcc ccgttgagga caccgagttg tcaggttgac catacgctta
     3721 agtgacaacc ccgctgcaac gccctctgtt atcaattttc tggtgacgtt tggcggtatc
     3781 agttttactc cgtgactgct ctgccgccgt ttttaaagtg aattttgtga tgcggtgaat
     3841 gcggctaagc gcacgcggaa cagttaaaac caaaaacagt gttatgggtg gattctctgt
     3901 atccggcgtt aattgttaac tggttaacgt cacctggagg caccaggcac tgcatcacaa
     3961 aattcattgt tgaggacgcg ataatgaaaa cgttattacc aaacgttaat acgtctgaag
     4021 gttgttttga aattggtgtc actatcagta accctgtatt tactgaagat gccattaaca
     4081 agagaaaaca agaacgggag ctattaaata aaatatgcat tgtttcaatg ctggctcgtt
     4141 tacgtctgat gccaaaagga tgtgcacaat gaattcagca tttgcgcttg ttctgacagt
     4201 ttttcttgtt tccggagagc cagttgatat tgcagtcagt gttcacagga caatgcagga
     4261 gtgtatgact gcagcaaccg aacagaaaat tcccggtaac tgttacccgg tcgataaagt
     4321 tattcaccag gataatatcg aaatcccggc aggtctttaa aacagttccg taataaatat
     4381 ccggtttcat tcttatatgc cagcaatggc agggatttgt tcatccttaa atctgtcatg
     4441 aggttaaaac aaaatgagta aagtctttat ttgcgccgct attcctgaca aactggcaac
     4501 aagggaagaa ggcgctgtgg ctgtagccac agccattgaa gctggcgacg aacgccgtgc
     4561 tcgagcaaaa tttcactggc agttcctgga acattatacg gctgctcagg actgcgctta
     4621 taaatttatt gtctgcgagg ataaacctgg catacctcgc cctgccctcg attcatggga
     4681 tgctgaatat atgcaggaaa accgctggga tgaggagtct gcttcttttg tcccggttga
     4741 gactaaatcc gatccgatga acgtcacttt tgacaagctg gcccctgaag tacagaacgc
     4801 tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt gatatggtta ttagcgcaca
     4861 ggaattgttg caggaagaca tggcaacatt cgacggacat atcgttgaag cgttgatgaa
     4921 aatgccagaa gttaacgcca tgtatccgga gcttaagttg cacgccattg ggtgggttaa
     4981 gcataaatgt attcctggtg ctaaatggcc cgaaattcag gcagagatgc gcatctggaa
     5041 aaaacgtcgc gaaggtgaac gcaaggaaac cggaaaatac acgtctgttg ttgatctcgc
     5101 ccgcaccaga gccaatcaac agtacactga aaattcaaca ggaaaaatca gcccggtcat
     5161 tgctgccatt catcgcgaat acaagcagac atggaaaaca ctggatgacg aactggccta
     5221 cgctctctgg cctggtgatg tggatgccgg aaacattgac ggcagcatcc atcgctgggc
     5281 aaaaaaagaa gttatcgaca acgaccgcga agactggaag cgtatctcgg catcaatgcg
     5341 caaacagcct gatgcccttc gctacgaccg ccaaactatt tttggccttg tccgtgagcg
     5401 tccgatcgac attcacaaag aacccgtagc actgaacaaa tatatctgcg aatacctgac
     5461 gacaaagggc gtgtttgaga atgaagaaac agacctgggc actgttgatg ttctccagtc
     5521 atcagaaaca caaactgatg cagtggaaac tgaggtatct gatatcccaa aaaatgaaac
     5581 cgcgccggaa gctgaaccat ctgtagagcg tgaggggccg ttctatttcc tcttcgcaga
     5641 taaggacgga gaaaaatacg gtcgcgcaaa caaactttct ggtctggata aggcactggc
     5701 tgctggcgcc actgaaatca ctaaagaaga atattttgcc cgaaaaaatg gcacatacac
     5761 gggcttaccg caaaatgtag ataccgctga agattcagaa caaccagaac cgataaaagt
     5821 taccgctgac gaagtaaaca aaattatgca ggcagccaat atcagccagc ctgacgccga
     5881 taagttgctt gctgcatcac gtggtgaatt tgttgaaggg attagtgacc cgaatgatcc
     5941 gaaatgggtt aaggggatcc agacccgcga ttctgtgaac cagaaccagc atgaatcgga
     6001 acggaactac caaaaagcgg aacaaaacag cccaaatgcg ttacaaaacg agccagaaac
     6061 gaaacagcct gaaccagtgg cgcaacagga agtggaaaaa gtctgcaccg cctgcggtca
     6121 gaccggcggc ggcaactgcc ctgattgtgg cgcggtgatg ggcgacgcaa cataccagga
     6181 aacattcgat gaagagtatc aggttgaagt tcaggaagat gatccgaagg aaatggaagg
     6241 cgctgaacat ccacacaagg ggaacactgg cggcaatcag catcacgata gcgataatga
     6301 aactggcgag acggcagatc actcaattaa ggtgaacggt catcacgaaa tcacatccac
     6361 cagcaggacg tgtgaccatc taatgatcga ccttgaaacc atgggaaaaa atcctgatgc
     6421 cccgatcatc tcaataggtg caatattttt cgatccgcaa accggagata tgggaccgga
     6481 atttagtaag actatcgatc tggaaactgc tggcggagtc attgatcggg acaccattaa
     6541 atggtggctt aagcaatcac gcgaagcgca atctgccatt atgaccgatg aaatcccgtt
     6601 agatgatgca ctgttacaat tgcgggaatt tatcgacgaa aactccggtg aattttttgt
     6661 tcaggtctgg ggaaatggag ccaacttcga caacacgatt ttgcgccgtt catacgaacg
     6721 gcaggggatc ccctgcccgt ggcgttacta caacgatcgc gatgtacgca caatcgttga
     6781 gctggggaaa gccatagact tcgatgccag aacggctatt ccattcgaag gtgagcgcca
     6841 taatgcactt gatgacgccc gttaccaggc aaaatacgtt tcagttatct ggcaaaaact
     6901 gatcccgagt caggctgatt cttaatgttc aactgtcgcc ggttgtgact ggtattctgc
     6961 aaccggcgct cgtctgatgt aagagataaa gaaatcgatg agcgaagtaa tcatgattgt
     7021 ctctcccggc aaatgggtat ccgaagagca gttaattgcg ctgaaaggaa taaaaaaagg
     7081 tacgttaaaa aaggcccggg aaaaatcgtt tatggaagga agggaatata agcatgtcgc
     7141 tcatgacggt atgccatggg ataacagtcc atgcttttac aacctggaag aaattgatcg
     7201 ctggattgag cgccaggcat cagcaagacc aagacgtcat cttacttgac taaaagccac
     7261 actaactaat gagagaagtt gaaatgaaat atccgacagg cgtggaaaac catggaggga
     7321 aattacgtat ctggtttgtt tataaaggcg taagagtcag ggaaaatctg ggggttcctg
     7381 acacagcaaa aaacaggcgc gttgcaggtg aactacgctc ctctgtttgt tacgcaataa
     7441 aaactggtgt tttcgactat gcaaaacagt ttccctcctc acgcaatctg gaaaaatttg
     7501 gtgaggcccg acaagattta accataaaag aactggctga aaaatttctg gcactgaaag
     7561 aaactgaagt cgccaaaaca tcactcaaca cataccgtgc cgtcatcaaa aatatcctga
     7621 gcataatcgg tgaaaaaaat cttgcctcat cgattaataa agaaaaatta ctggaggttc
     7681 gtaaagagct actgactgga taccagatcc ccaaaagtaa ctatattgtt acacaaccag
     7741 ggagatcggc tgtaactgta aataattaca tgacaaatct taacgccgtg ttccagtttg
     7801 gtgttgataa cggttacctg gcagataatc cgtttaaggg gatctcgcca ttaaaggaat
     7861 caagaaccat tccggatcct ctttcgcggg aggaatttat ccgtcttatc gatgcgtgca
     7921 gaaatcagca agcaaaaaat ttatggtgtg tttctgttta tactggcgtt cgccctggtg
     7981 agctgtgtgc acttggatgg gaggacatag atctgaaaaa tggaacaatg atgatcagga
     8041 gaaatttagc aaaagaccgt ttcacggtac caaaaacaca ggcgggaacc aatcgggtca
     8101 ttcatcttat taagccagca atcgacgctc tccggagtca gatgacatta acgagactga
     8161 gtaaagagca tatcattgat gttcacctca gagagtatgg cagaacagaa aaacaaaaat
     8221 gcacctttgt ttttcaacct gaagtgtcag cgagagtaaa aaattatggt gaccatttta
     8281 ccgttgactc aataaggcag atgtgggacg cagcgataaa acgtgccgga ctccgccatc
     8341 gaaaatcata tcagtcgaga catacttatg cctgctggtc gctgacagct ggtgccaacc
     8401 cggcatttat agcaaaccag atgggccatg cagatgcgca aatggtattt caggtatacg
     8461 gaaaatggat gtctgaaaac aataatgcac agctagcttt gttaaataca cagttaagcg
     8521 agttt
//
LOCUS       DAPGWD010000176         8410 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPGWD010000176 DAPGWD010000000
VERSION     DAPGWD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.177"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(189..265)
                     /locus_tag="RHX88_003855"
     tRNA            complement(189..265)
                     /locus_tag="RHX88_003855"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Asp,seq:gtc)
     gene            complement(398..1129)
                     /gene="dnaQ"
                     /locus_tag="RHX88_003856"
     CDS             complement(398..1129)
                     /gene="dnaQ"
                     /locus_tag="RHX88_003856"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDV2232490.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1194..1661
                     /gene="rnhA"
                     /locus_tag="RHX88_003857"
     CDS             1194..1661
                     /gene="rnhA"
                     /locus_tag="RHX88_003857"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDV2232491.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1658..2380)
                     /locus_tag="RHX88_003858"
     CDS             complement(1658..2380)
                     /locus_tag="RHX88_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV2232492.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2414..3169
                     /gene="gloB"
                     /locus_tag="RHX88_003859"
     CDS             2414..3169
                     /gene="gloB"
                     /locus_tag="RHX88_003859"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDV2232493.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3241..4599
                     /gene="mltD"
                     /locus_tag="RHX88_003860"
     CDS             3241..4599
                     /gene="mltD"
                     /locus_tag="RHX88_003860"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDV2232494.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NMPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4647..5417)
                     /locus_tag="RHX88_003861"
     CDS             complement(4647..5417)
                     /locus_tag="RHX88_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV2232495.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DTSVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5495..6295)
                     /locus_tag="RHX88_003862"
     CDS             complement(5495..6295)
                     /locus_tag="RHX88_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV2232496.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6536..7450
                     /locus_tag="RHX88_003863"
     CDS             6536..7450
                     /locus_tag="RHX88_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2232497.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7447..8250)
                     /gene="dkgB"
                     /locus_tag="RHX88_003864"
     CDS             complement(7447..8250)
                     /gene="dkgB"
                     /locus_tag="RHX88_003864"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDV2232498.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2253 a         2006 c         2039 g         2112 t
ORIGIN      
        1 tctggaaata tatttaagct gaatggttcg atatcattat tgaattttaa tgaatgagac
       61 aacaagcgag aatcacgcac ttacgccaga ataaaatcat tgaacagaga aatggtggaa
      121 ttcaaataca aaaaaaccgc aaaattaaaa atcttgcggc tctctgaact cattttcatg
      181 agtgaatttg gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta
      241 ttctaaccga ctgaactacc gctccgcgtt gtgttccgtt gggaacgagg cgaatattac
      301 ggattgcctc acccttcgtc aacggttttt ctcatctttt gaatcgtttg ctgcaaaaat
      361 cgcccaagcc gctattttta gcgcctttta caggtattta tgcccgccag aggcagcttc
      421 cgcccttctt ctccaccaga tcaagacggg cttcatgagc tgcaagctct tcatctgtcg
      481 caaaaacaac gcgtaactta cttgcctgac gtacaatgcg ctgaattgtt gcttcacctt
      541 gttgctgctg tgtctctcct tccatcgcaa aagccatcga cgtttgacca ccggtcatcg
      601 ccagataaac ttccgcaagg atttgggcat cgagtaatgc cccgtgcagc gttcgtttac
      661 tgttatctat ttcgtagcgg gcacataacg catcgaggct gttacgttta ccgggaaaca
      721 ttttcctcgc caccgcaagg ctatcggtga ccttacagaa ggtattagtc ttcggaatat
      781 cgcgcttaag caacgagaac tcgtagtcca taaagccgat atcgaacgct gcgttatgga
      841 tcaccaactc cgcgccgcgg atatagtcca tgaactcatc ggctacttcg gcaaacgtgg
      901 gcttatcgag caaaaattca tcggcaatac catgtacgcc aaaggcttcc ggatccacca
      961 gccgatcggg tttgagataa acatggaagt tattgcccgt caggcgacgg ttcaccactt
     1021 caacggcacc aatctcaatg atcttgtggc cttcatagtg cgcaccaatc tggttcatac
     1081 cggtggtttc ggtatcgaga acgatctggc gtgtaattgc agtgctcata gcggtcattt
     1141 atgtcagact tgtcatttta cagttcgatt caattacagg aagtctacca gagatgctta
     1201 aacaggtaga aattttcacc gatggttcgt gtctgggtaa tcctgggcct gggggttacg
     1261 gcgctatttt acgctatcgc ggacgcgaga aaacctttag cgcaggctac acccgcacca
     1321 ccaacaaccg tatggagttg atggccgcta ttgtcgcgct ggaggcgtta aaagaacatt
     1381 gcgaagtcat tttgagtacc gacagccagt atgtccgcca gggtatcacc cagtggatcc
     1441 ataactggaa aaaacgtggc tggaaaacag cagacaaaaa accagtaaaa aatgtcgatc
     1501 tctggcaacg tcttgatgct gcattggggc agcatcaaat caaatgggaa tgggttaaag
     1561 gccatgccgg acacccggaa aacgaacgct gtgatgaact ggctcgtgcc gcggcgatga
     1621 atcccacact ggaagatacg ggctaccaag tcgaagttta agcctgtggt ttacgacatt
     1681 gccgggtggc tccaaccgcc tggcgaattc gtggcttgtt tttactctgt ttcatcggat
     1741 ttagcgttaa aggaatagtc cgtttccggg caacaataag ttgtaagcaa ccaagcgcag
     1801 gaatatgcgc attcaatagt tttcctccgt gtttgttcca cgggagaacg tggaaacggc
     1861 tggcgtgtag cacttcaaaa ttcaacaaag agagccagtc cagttgccgc atcagagtaa
     1921 acatccggct attatagggc gaggttttgc gcaataccgg cacaagtttg cgtaatccca
     1981 ttaaactgat gggattgaag ccactaatga ccagccagcc atcatcaatc aatacccgat
     2041 cggcttcacg caataaacga tgcggatcgg tgcaccacgg caatgtatgt gccagtagac
     2101 aaacatcaac ggatttatcg gcaaaaggaa gatgaagtgg gtccgcctgt acctggacgg
     2161 gcattccttg cgcagaaaca ttcacttgat gagaaaccgc gcacgcttcg caattgattt
     2221 ctgcgcttaa attgccaatc ttaagcagat gaaaaccata cattttagtg aaccacgggt
     2281 tgagctggcg ctccagcgcc ttgcgataaa gctctcccca gggcaaatcg ccccagcaat
     2341 caggagccac gacagtttga gggactcttg ccggtttcat cacaaccttc cgtttcacac
     2401 taagaggtaa tctatgaatc ttaacagtat tcccgccttt gatgacaatt acatctgggt
     2461 tttgaatgat gaagcaggtc gctgcctgat tgtcgatccc ggagacgcag agccagtatt
     2521 aaacgccatt accgccaata actggcaacc ggaggccata tttctcaccc accatcatca
     2581 cgatcacgtt ggcggcgtaa aagaactggt ggaaaagttt ccgcaaattg tggtgtatgg
     2641 tccacaagag acacaagata agggaacaac acaggtagtc aaagatggcg aaactgcctt
     2701 cgttttgggg catgaattta gtgtaattgc cacaccgggt cacactttag gacatatctg
     2761 ttacttcagt aaaccttatc tattttgcgg cgacacgctg ttttctggtg ggtgtggtag
     2821 gttgtttgaa gggacagcat cacaaatgta tcaatcactt aataagttaa gtgcgttacc
     2881 tgacgataca ttggtatgtt gtgctcatga atatacgtta tcaaatatga agtttgcttt
     2941 gagtattctt ccgcacgatt tgtccataaa tgattattat cgtaaagtta aggagttacg
     3001 ggcaaaaaat caaataacac tacccgtaat tctgaaaaat gagcggcaaa ttaatgtttt
     3061 tttaagaaca gaagatattg atttaattaa tgtaattaat gaagaaacat tattgcaaca
     3121 acctgaagaa cgttttgcat ggttaaggtc aaagaaagat aggttctgat aaaactttct
     3181 tgtcatcggc tccgttcgcc gttatgatcg gtcgtctttt aagcaactat tgacacacac
     3241 atgaaggcaa aagcgatatt actcgcctct gtcctgctcg tgggttgcca gagtaccggc
     3301 aacgttcaac agcacgcaca gagcctttct gcagctggtc aaggggaagc agcaaagttt
     3361 acaagtcagg cacgatggat ggacgatggg acgtctatcg cgccagatgg tgacttgtgg
     3421 gctttcattg gcgacgagct aaagatggga attccggaaa atgaccggat tcgcgaacag
     3481 aaacagaaat atttacgcaa taagagctat ctccacgatg taactttacg ggcagagccg
     3541 tatatgtact ggatagccgg gcaagttaaa aaacgtaaca tgcctatgga actggtacta
     3601 ctacccatag tggagagcgc ttttgatcct cacgcaacgt ctggcgccaa tgccgcaggc
     3661 atctggcaga tcattccgag cacggggcgc aattatggtt tgaaacagac ccgcaattat
     3721 gacgcgcgtc gcgatgttgt tgcttcaaca actgccgcgc tgaatatgat gcagcgtctg
     3781 aacaaaatgt ttgacggcga ctggcttctg accgtagcgg cttataacag cggcgaaggt
     3841 cgggtcatga aggcaattaa aacgaacaaa gcgcgtggga aatccacgga cttctggtcg
     3901 ttaccgttgc cgcaggaaac gaagcagtac gtgcctaaaa tgctggcatt gagtgatatt
     3961 ctcaaaaaca gcaagcgtta tggcgtacgt ctgccaacga ccgatgaaag ccgtgctctg
     4021 gcgcgtgtgc acctgagcag cccggttgaa atggcgaagg ttgcagatat ggcggggatt
     4081 tccgtcagca agctgaagac attcaacgct ggcgtgaaag gctccacgct gggcgcaagt
     4141 ggtccgcaat acgtgatggt gccaaagaag catgcagatc aactgcgtga atctctcgct
     4201 tcaggcgaaa ttgctgctgt acagtcgacg ctggttgccg acaatatgcc gcttaacagc
     4261 cgagtttaca ccgtacgctc tggcgacacg ctttcaagta tcgcttcacg tctcggcgta
     4321 agcaccaaag atttgcagca gtggaacaaa ctgcgcggat ctaagctgaa gccaggccaa
     4381 agtttgacga ttggtgcagg cagtagcgca cagcgactgg caaacaacag cgatagcatt
     4441 acgtatcgtg tgcgcaaagg cgattcgctt tcaagcattg ctaaacgcca cggcgtgaac
     4501 atcaaagatg tgatgcgctg gaacagcgac actgcgaatc tgcaaccagg cgataagctg
     4561 acgttgtttg tgaaaaacaa caacatgcca gattcctgac aaaccagata ataaaaaggc
     4621 accgattccc ccggtgcctt ttttatttat gctgctttat gtgcttctac catgatgata
     4681 tcactggtga aagagccatc attctgcaag gcaaaatacg ttctcacctc tgtcgatgcg
     4741 ctctgttggt aaatgcgaat agcgtctact aacgcttctg gcgtacgcat tctcgcgacc
     4801 catgaagaaa attccagcgg taacttatct gtaattaaat tatcaactat cagattggct
     4861 tcattgatta acctcaacca ctcaccgctg gcgtagtttc gtacgtgaga ggtatcgcgt
     4921 aatgcttcta ccgtctgtaa ccagatgtcg cgcactggat gacccggaga cattacgtcc
     4981 atcacaatca tcctaccgcc aggtttcaat atccggttca cttcacgcag tgctgcacca
     5041 acatcatgcc agtgatgggc agaataacgg ctgataacaa tatcaaatgc gttatcggca
     5101 aatggcagac tttcggcata accctggcgg gtggtgatat ttttcagttg ccgggcttcg
     5161 gcagcttgtg ccacgacatc cagcatttgg gcagataagt catacgccac caccgcgctc
     5221 acgttctgcg cagcgacaaa gctggcatgc cctgctccgc agcccatatc aagtacactt
     5281 gtatcaggat aatcagccag acgcaccgcc aggcgctgta aatctcgccc ggatgcatgt
     5341 acggtactgg ttaaatactc actggcctgc gagctaaact gcttttctac gtggtcatgg
     5401 tgggattgtg ttgtcattgc gctgtccttt tgttgtttga gtataaaagg gcagcgccaa
     5461 acgttggtct gccctgtggc agacctgaca taccttattt atcaggcttg ccgggactga
     5521 attcaacgag tagcggattg tgatcggaag cgcgcgtaac cagtacagaa gcttcgctga
     5581 cgttcagacc acggtagaaa acaaaatcga gcggacggcc aaacgcccga cggcgctgat
     5641 cgtcggtaaa acgcacctgg cgcagcgaca tttcccgcgc aaagcgatat aacgcgttca
     5701 tccttctacg gctccaggca ttgaaatctc ctgccataat gaccgggccg ctgtggtgag
     5761 ctatctgatc accaatagga agtaactgct tactatagac atccacgccc agactgaagt
     5821 tgacggcgtg tatattaacc accatcaaca ggcgggtgtc aggtaatgga tagaccgtca
     5881 ccagtgccga cttcgccaga cgcaaaatgg gttctcgttc acgtaacggg cagcaataca
     5941 ctggatgcgc cgccgaaagg gtcattacgc cagaaggatg ttgtggcagt acgaaagcgg
     6001 gtacctgatc ggcggcaaga tagttagcgg tcgcaaactg tactaactct ggcgttgtct
     6061 gcgcttcctg caataacacc agatgtgcat ctttgccgta gttctttaat accgacaacc
     6121 attcagcgcg ttgctgtttg tatatgttcc acaccaggat ccgaatacgc tcttcggtac
     6181 ttaacggttc tccgggtggt aatgcctggc cgatgctcgc aaaagacccc ggcggtaaga
     6241 tcctttccgc aggttgtccg gcaacatagc gcatggcata ggtgtttttt cgcacttttg
     6301 actttgaaac ctctattacg tcaacccttc cgcactcggg agggttctac tgttataggg
     6361 attttagctc actctttcaa cgagcaattg tatattgtta tgtaagcaag tgcttacacc
     6421 ggtcagaatt ttgctcatta tggagaacat taaatcgcat gacatatcac gcatcctgat
     6481 ggtttaatga caaataaatt caaaatatct cttctatttt gcaacaggag caaaaatgaa
     6541 agccacgtcg gaagaactcg ccatttttgt ttcggtcgtc gaaagcggca gctttagccg
     6601 ggcagcggaa caattagggc aagcaaactc agcggtaagc cgggcggtga aaaagctgga
     6661 gatgaaactt ggcgttagcc tgcttaatcg gaccacgcga caacttagcc tgacggaaga
     6721 aggcgagcgt tattttcgtc gcgtacagtc aattttgcag gagatggcat cggcagaatc
     6781 agaaattatg gagacgcgta atacaccgcg tggactgtta cggatcgatg ccgcaactcc
     6841 agtggtgctg cactttctga tgccgttaat taagcctttc cgtgaacgct atccggaagt
     6901 cactttatcg ctagtctcct ccgaaacgat tattaatttg atcgaaagaa aagtggatgt
     6961 cgcgatacgc gctggtacgt taacggattc cagcttacgt gccaggccgt tatttaacag
     7021 ttatcgaaaa attatcgcct cccccgatta tatttcccgc tacgggaagc cagaaacgat
     7081 cgacgattta aagcaacata tttgcctggg attcactgaa cccgcttccc tcaatacctg
     7141 gccgatagcc cgtagcgatg gacaattaca tgaggtgaag tacggtttgt catccaatag
     7201 tggggaaaca ctgaaacagc tttgcctgag tggcaacggg attgcgtgtt tgtccgacta
     7261 catgatcgac aaagaaatcg ctcgcggaga attggtggag ttaatggcag ataaagtgtt
     7321 gccagtggaa atgccattca gtgccgtcta ttacagcgac cgtgcggtaa gtacgcgcat
     7381 ccgggctttt atcgatttcc ttagcgagca tgtaaaaaca gctcccggag gagctgtcag
     7441 agaggcttaa tcccattcag gagccagacc ttccgggcta accaggcggt cgttgcaatc
     7501 cagtgcggcg atcgcttttt tatcttcggc atcaagctgt aaattttgtg ccttaagatt
     7561 actttccagg tttttacgtt tagtagaaga aggaattact gagtaacctt cccccatagc
     7621 ccacgccaga atcacttgtg ccggagtcgc attgtgttta gctgcgatac gagcaataac
     7681 ctcatctttc agggccttac catacgccag cgtcatatag gaagtaatat ggatgccgtg
     7741 ctgtttagcc caggcaacca ctttacggtt ttgcagataa ggagagagtt caatctggtt
     7801 agtagcgatg ttttcagcac caacagcagc aatcgctttt tccatcaacg ggatcgtgaa
     7861 gttggaaata ccgatctcac gcgtcagccc ttgttttttg gcttccagca gcgcctgcat
     7921 aaactcttca acagagactt catcgtttgg tgacggccag tggattagcg tcagatcaac
     7981 ataatcggta cgcaattttt gcaggctctc tttcagactt gggatcaatt tgtctttgct
     8041 gagattttca atccagattt tagtggtgat gtagagttca tgacgtggca cgccactttc
     8101 tgcaatcgcc tgacctactg cggcttcgtt atcatagatt tgtgcggtat caattgcgcg
     8161 ataaccaagt tcaagcgccg ttatcacaga tgaaataaca acgtcgtctt tcagacggaa
     8221 agtacctaaa ccaaatgcag ggatagccat aatatgcctc ttttaatgct attttcgtta
     8281 actgaggtga ttatgggctt cagactgttg aggaaaaaga gcgtaaaatg cagaggattt
     8341 ttgcgattct ggcaataata gatatacaga cgaaaaaaaa cctcgagcgt taactcgagg
     8401 cttcttaata
//
LOCUS       DAPGWD010000177         8406 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPGWD010000177 DAPGWD010000000
VERSION     DAPGWD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.178"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            301..1278
                     /gene="csiD"
                     /locus_tag="RHX88_003865"
     CDS             301..1278
                     /gene="csiD"
                     /locus_tag="RHX88_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDV2232499.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1298..2566
                     /gene="lhgO"
                     /locus_tag="RHX88_003866"
     CDS             1298..2566
                     /gene="lhgO"
                     /locus_tag="RHX88_003866"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDV2232500.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2589..4037
                     /gene="gabD"
                     /locus_tag="RHX88_003867"
     CDS             2589..4037
                     /gene="gabD"
                     /locus_tag="RHX88_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV2232501.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4051..5331
                     /gene="gabT"
                     /locus_tag="RHX88_003868"
     CDS             4051..5331
                     /gene="gabT"
                     /locus_tag="RHX88_003868"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDV2232502.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5642..7042
                     /gene="gabP"
                     /locus_tag="RHX88_003869"
     CDS             5642..7042
                     /gene="gabP"
                     /locus_tag="RHX88_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDV2232503.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7063..7725
                     /gene="csiR"
                     /locus_tag="RHX88_003870"
     CDS             7063..7725
                     /gene="csiR"
                     /locus_tag="RHX88_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDV2232504.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7726..8175)
                     /gene="kbp"
                     /locus_tag="RHX88_003871"
     CDS             complement(7726..8175)
                     /gene="kbp"
                     /locus_tag="RHX88_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDV2232505.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8259..>8406)
                     /locus_tag="RHX88_003872"
     CDS             complement(8259..>8406)
                     /locus_tag="RHX88_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDV2232506.1"
                     /translation="RIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIHAFWV
                     QTRD"
BASE COUNT         1880 a         2193 c         2367 g         1966 t
ORIGIN      
        1 ataatatgtt tagtttacta acggtcattt tgcagtgaag ccatttactg ttttttatcg
       61 accagataat ctgttctcta atgttaactc cccctaacct gttgctttag ttattcattt
      121 cctgtctcac tttgccttaa taccctacgt taaatgttac taatttgttg cttttgatca
      181 caataagaaa acaatatgtc gcttttgtgc gcatttttca gaaatgtaga tatttttaga
      241 ttatggctac gaaatgagca tcgccatgtc accctacatc tcataagagg atcgcttctg
      301 atgaatgcac tgaccgccgt acacaataac gctgtcgatt caggccagga ctatagcgga
      361 ttcactctca tcccgtcggc gcaatccccg cgtctgctgg aactcacctt caccgaacag
      421 acgaccaaac agtttctcga acaggttgcc gaatggcccg tgcaggcgct ggagtacaaa
      481 tcgtttctgc gttttcgggt aggcaaaatt cttgacgatc tgtgtgcgaa tcagctgcaa
      541 ccattgctgt tgaagaccct gctaaaccgt gctgaaggtg cgctgttgat caatgcggtg
      601 ggtatcgatg atgtggcgca ggcagatgag atggtgaagc tggcaacggc ggtagcgcat
      661 ctgattggtc gctcaaattt cgatgcgatg agcggtcagt attacgcgcg tttcgtggtg
      721 aaaaatgtcg ataactcaga cagctatctg cgtcagccgc accgcgtgat ggaactgcac
      781 aacgacggca cgtatgtcga agagatcacc gattacgtgc tgatgatgaa aatcgatgag
      841 caaaacatgc agggtggaaa ttcgttgctg ctgcatctcg atgactggga acatctggac
      901 cactatttcc gccatccact ggcgcgtcgc ccgatgcgct ttgccgcgcc gccgagcaaa
      961 aacgtcagca aagatgtttt ccatccagtg ttcgacgttg atcaacaggg tcgcccggtg
     1021 atgcgctata tcgaccagtt cgtccagcca aaagattttg aagaaggcgt gtggttgagc
     1081 gagctttccg atgccattga aaccagcaaa ggcattcttt ctgtacccgt tcccgttggc
     1141 aaattcctgt tgataaacaa cctgttctgg ctgcacggtc gcgaccgctt tactccgcac
     1201 ccggatctgc gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc cactcaccac
     1261 taccagacgc atcagtaagc gcaaaggaat tgagcggatg tatgattttg tgattattgg
     1321 cggcggcatc atcggcatgt cgaccgccat gcaactgatt gatgtttatc cggatgcccg
     1381 cattgcgttg ctggaaaaag agtccggccc ggcctgtcac cagacgggcc acaacagcgg
     1441 cgtgatccat gccggggtct attacacgcc cggcagcctg aaggcgcagt tttgcctggc
     1501 gggaaaccgc gccaccaaag ccttttgcga tcaaaacgac attcgctatg acaactgcgg
     1561 caagatgctg gtcgccacgt ccgatctcga aatggaacgg atgcgcgcgt tgtgggaacg
     1621 cacggcggcg aacggtatcg agcgcgagtg gttaaacgcc gaggaactgc gcgagcgcga
     1681 accgaatatc accgggctcg gcggcatttt tgtgccgtcc agcggcattg tcagctaccg
     1741 cgaagtaacg gcggcgatgg caaaaatttt ccaggccaga ggcggcgaga ttatctataa
     1801 cgccgaagtc agcggcctta gtgagcataa aagcggcgtg gtgatacgta cccgtcaggg
     1861 cggcgactat gaagcatcga cgctgattag ctgttccggg ctgatggctg accggctggt
     1921 gaaaatgctc ggcctcgaac cgggctttat catctgcccg ttccgcggcg agtatttccg
     1981 cctggcgccg gagcataacc agattgttaa ccatctgatt taccccattc ccgacccggc
     2041 aatgccgttt ttgggcgtgc atctcacccg aatgatcgat ggcagcgtca ccgtcgggcc
     2101 aaacgcggtg ctggctttca aacgcgaagg ctatcgcaag cgcgacttct catttagcga
     2161 cacgctggag attttgggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc
     2221 aggactgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcgcc tggtgcaaaa
     2281 gtattgtccc cggctttcgt taagcgatct ccagccctgg cccgccggtg tgcgggcgca
     2341 ggcagtatcg ccggacggca agctgattga cgattttctg tttgtcacca ccccacgcac
     2401 gatccacacc tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca
     2461 tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg
     2521 agcggcacgt agtgtggatg ccttacacgc cgcatttaat caataacctt tgaaaacagg
     2581 acgtaacaat gaaacttaac gacagtaact tattccgcca gcaggcgttg attaacgggg
     2641 aatggctgga cgccaacaat ggtgaagtca tcgacgtcac caatccggcg aacggcgaca
     2701 agctgggtag cgtgccgaaa atgggcgctg atgaaacccg cgccgctatc gacgccgcca
     2761 accgcgccct gcccgcctgg cgtgcgctca ccgccaaaga acgcgccaac attctgcgca
     2821 actggttcaa tttgatgatg gagcatcagg acgatttagc gcgcctgatg accctcgaac
     2881 agggcaaacc actggccgaa gcgaaaggtg aaatcagcta cgccgcctct tttattgagt
     2941 ggtttgccga agaaggcaaa cgcatttatg gcgacaccat tcccggtcat caggccgata
     3001 aacgcctgat tgttatcaag cagccgattg gcgttaccgc agctatcacg ccgtggaact
     3061 tcccggcggc gatgattacc cgtaaagccg gtccggcgct ggcggcaggc tgcaccatgg
     3121 tgctgaagcc cgccagtcag acaccgttct ctgcgctggc gctggcggag ctggcgattc
     3181 gcgcgggcat tccggctggc gtgtttaacg tggtcaccgg ttcggcaggc gcggtcggta
     3241 atgaactgac cagcaacccg ctggtacgca aactgtcgtt taccggttcg accgaaattg
     3301 gccgccagtt aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg gagctgggcg
     3361 gcaacgcgcc gtttatcgtc tttgacgatg ccgatctcga taaagccgtg gaaggcgcgc
     3421 tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt ttatacgtgc
     3481 aggacggcgt gtatgaccgc tttgccgaaa aattgcagca ggcggtgagc aaactgcaca
     3541 tcggcgacgg gctggataaa ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag
     3601 caaaagtgga agagcatatt gccgatgcgc tggagaaagg tgcgcgtgtg gtttgcggcg
     3661 gtaaagcgga tgaacgcggc ggcaacttct tccagccgac cattctggtg gacgtgccgg
     3721 ccaacgccaa agtgtcgaaa gaagagacgt tcggccccct cgccccgctg ttccgcttta
     3781 aagatgaagc cgatgtgatt gcgcaagcca atgacaccga gtttggcctt gccgcctatt
     3841 tctacgcccg tgatttaagc cgcgtcttcc gcgtgggcga agcgctggag tacggcatcg
     3901 tcggcatcaa taccggcatt atttccaatg aagtggcccc gttcggcggc atcaaagcct
     3961 cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata
     4021 tgtgcatcgg tctttaactg gagaatgcga atgaacagca ataaagagtt aatgcagcgc
     4081 cgcagtcagg cgattccccg tggcgttggg caaattcacc cgattttcgc tgaccgcgcg
     4141 gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc gggcgggatt
     4201 gcggtgctca ataccgggca cctgcatccg aaggtggtgg ccgcggtgga agcgcagttg
     4261 aaaaaactgt cgcacacctg cttccaggtg ctggcttacg agccgtatct ggagctgtgc
     4321 gagattatga atcagaaggt gccgggcgat ttcgccaaga aaacgctgct ggttacgacc
     4381 ggttccgaag cggtggaaaa cgcggtaaaa atcgcccgcg ccgccaccaa acgtagcggc
     4441 accatcgctt ttagcggcgc gtatcacggg cgcacgcatt acacgctggc gctgaccggc
     4501 aaggtgaatc cgtactctgc gggcatgggg ctgatgccgg gtcatgttta tcgcgcgctt
     4561 tatccttgcc cgctgcacgg cataagcgag gatgacgcta tcgccagcat ccaccggatc
     4621 ttcaaaaatg atgccgcgcc ggaagatatc gccgccatcg tgattgagcc ggttcagggc
     4681 gaaggcggtt tctacgccgc gtcgccagcc tttatgcagc gtttacgcgc gctgtgtgac
     4741 gagcacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg
     4801 ctgtttgcga tggagcagat gggcgttgcg ccggatctca ctacctttgc gaaatcgatc
     4861 gcgggcggct tcccgctggc gggcgtcacc gggcgcgcgg aagtgatgga tgccgtcgct
     4921 ccaggcggtc tgggcggcac ctatgccggt aacccgattg cctgcgtggc ggcgctggaa
     4981 gtgttgaagg tgtttgagca ggaaaatctg ctgcaaaaag ccaacgatct ggggcagaag
     5041 ctaaaagacg gattgttggc gatagccgaa aaacacccgg agatcggcga cgtacgcggg
     5101 ctgggagcga tgattgccat cgagctgttt gaagatggcg atcacagcaa accggacgca
     5161 aaactcaccg ccgagatcgt ggcacgcgcc cgcgataagg gcctgattct tctctcctgc
     5221 ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca ccattgaaga cgctcagatc
     5281 cgtcagggtc tggagatcat cagccagtgt tttgctgagg cgaagcagta gctatgtccg
     5341 tttgtaagag ttaaacgttg ctaagggaat gataacgatg cagtaaaaaa taataacgat
     5401 gaggcgatcg acaaagttaa aaacgactgc ggactgtccc ctcgctcctc tggggagagg
     5461 gttagggtga ggggtaatca cgcggctaca ccctcacccc ggccctctcc ctaagggaga
     5521 gggagaaaac tctcccgctt tcgtttttaa gattttgttg accgtctcaa ttaaacgcgt
     5581 gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga gaggattcag
     5641 gatggggcaa tcatcgcaac cacatgagtt aggcggcggg ctgaagtcac gccacgtcac
     5701 catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca gcgtcgccat
     5761 cgccgaagcg ggcccggcgg tattactggc ctatctgttc gccggattac tggtggttat
     5821 gattatgcgg atgttggcgg aaatggcagt tgccacgccc gataccggtt cgttttccac
     5881 ctatgccgat aaagccattg gccgctgggc gggttatacc atcggctggc tgtactggtg
     5941 gttttgggta ctggttatcc cgctggaagc caacatcgcc gctatgatcc tgcactcgtg
     6001 ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct taactggcag
     6061 taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc tgtgcaaagt
     6121 catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt tttaccctta
     6181 tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc ccaacggctt
     6241 cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg cagaaattgt
     6301 caccattgcc gccgcggaat ccgacacgcc ggaaaaacat attgtccgcg ctaccaactc
     6361 ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtag tggcgttaat
     6421 accgtggaat atgccggggc tgaaagccgt tggttcttat cgctcggttc tggaattgct
     6481 caatattccc catgcgaaat taatcatgga ctgcgtgata ttactttccg taaccagctg
     6541 tctgaactcg gcgctgtata ccgcgtcaag gatgctctac tccttaagcc gtcgcggtga
     6601 tgcgcccgcg gtaatgggca aaatcaaccg cagtaaaacc ccgtatgtgg cggtgttact
     6661 ctccaccgga gcggcatttt taacggtggt ggtgaactat tacgcacctg cgaaagtgtt
     6721 taaattcctg atagacagct ccggtgctat cgccctgctg gtttatttag tcatcgccgt
     6781 ttcacagttg cggatgcgta aaattctgcg agcagaagga agcgaaattc gcttgcgcat
     6841 gtggctttac ccgtggctca cctggctggt aataggcttt attacctttg tgttggtggt
     6901 gatgttattc cgcccggcgc aacagttaga agtgatctct accggcttat tagcgatagg
     6961 gattatctgt accgtgccga ttatggcgcg ctggaaaaag ctggtattgt ggcaaaaaac
     7021 acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat tacgtctctg
     7081 gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc ggatgaaaaa
     7141 ttacgaatga gtttgctgac atcgcgttat gcacttggcg ttgggccgtt acgggaagct
     7201 ctttcgcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg gtatcgggtg
     7261 gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa tatggaagcg
     7321 atgttagtga gtctggcgat tgcccgcggt ggcgatgagt gggaggcaga cgttctcgca
     7381 aaagcgcatc tgctgagtaa gcttgaggcc tgtgacgcca gcgagaaaat gcttgatgag
     7441 tgggatctgc gtcatcaggc gtttcatacg gcaattgtgg cgggctgtgg ttctcactat
     7501 ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt tatctggctg
     7561 cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca ccagaccctg
     7621 actgcggcgg tactggcgcg agataccgcg cgcgccagtg agttaatgcg ccagcattta
     7681 ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact cttccggaat
     7741 acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt tattcgcttc
     7801 gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg cactcagagt
     7861 gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg ttttcacctg
     7921 atcatcgaca ctggcaatac cggaaatatt ccccaccgca acaaggattt tctccttcgc
     7981 ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt gaatattcac
     8041 tttatcggca tccggtatac cggttttgtt cagatgctcc tgcaccttct tcgcctgatc
     8101 gtctttatcg tgctgacctg taaccgcgtc ccagagtttt tctccggcat ctttcacaaa
     8161 attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag tgtagcagag
     8221 gaacgcggcg cgttaacgcc gcggggatga gcacaggttt aatcgcgggt ttgcacccag
     8281 aacgcgtgaa tcaaaccggg gatataccca agcagtgtca acagaatatt aataatgaac
     8341 gcccaaccga accctttacc gagcagcacg ccgagcggcg gcagaatgat ggtgatgacg
     8401 attctc
//
LOCUS       DAPGWD010000178         8387 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPGWD010000178 DAPGWD010000000
VERSION     DAPGWD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.179"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..250
                     /locus_tag="RHX88_003873"
     CDS             <1..250
                     /locus_tag="RHX88_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV2232507.1"
                     /translation="FSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGL
                     TCEADTLGSCSFVYMAVYATRFSNQLPKASVHQLTHYF"
     gene            complement(792..2039)
                     /locus_tag="RHX88_003874"
     CDS             complement(792..2039)
                     /locus_tag="RHX88_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HDV2232508.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYSGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2111..3025)
                     /locus_tag="RHX88_003875"
     CDS             complement(2111..3025)
                     /locus_tag="RHX88_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDV2232509.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            3241..4674
                     /gene="cscA"
                     /locus_tag="RHX88_003876"
     CDS             3241..4674
                     /gene="cscA"
                     /locus_tag="RHX88_003876"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDV2232510.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(4682..5740)
                     /locus_tag="RHX88_003877"
     CDS             complement(4682..5740)
                     /locus_tag="RHX88_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2232511.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            5920..6138
                     /locus_tag="RHX88_003878"
                     /pseudo
     CDS             5920..6138
                     /locus_tag="RHX88_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            6156..7484
                     /gene="dsdA"
                     /locus_tag="RHX88_003879"
     CDS             6156..7484
                     /gene="dsdA"
                     /locus_tag="RHX88_003879"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDV2232512.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(7592..>8387)
                     /gene="emrY"
                     /locus_tag="RHX88_003880"
     CDS             complement(7592..>8387)
                     /gene="emrY"
                     /locus_tag="RHX88_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDV2232513.1"
                     /translation="LISLVIWESTSENPILDLSLFKSRNFTIGIVSITCAYLFYSGAI
                     VLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNKIDMRVLVTFSFLMYAV
                     CYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFANASSMSNF
                     FRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSSSQIMDKYYGSLSGVLN
                     EINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG"
BASE COUNT         2370 a         2078 c         1928 g         2011 t
ORIGIN      
        1 gttcagcgat gcggatgcat accatctgga ccaggccttt ccactgctga tgaaacaact
       61 ggaactcatg ctcaccagcg gtgaactgaa tccccgccat cagcatacca tcacgctgta
      121 tgcaaaaggg ctgacctgtg aagctgatac tctcggcagt tgtagtttcg tttatatggc
      181 tgtttatgct acccgattct caaatcaatt accgaaagct tctgttcatc aactaactca
      241 ttacttttaa tgatggggcc ctttctgggg cccaagaaat taacaacgct aacaaacaac
      301 ataaataaca ataaattaaa taaaaaattt cattcctgca ggggacacca gatacccttc
      361 aaacgaaatc taccttcacc ccgtaaaaga tgggtttggc agcacacttg ccttatatct
      421 actcattttt actgcaacag gttgaaatct cagcactgtc agaaagcgct gatgactaaa
      481 cagccctgag ccgggcgatg taaccatcac acagaatcct gatagcgaaa tatggcgtga
      541 ctcgatactt cactccgcaa tgcattcctt gatgaattcg caggaccgtg atacacggga
      601 caggtcactg aatgacgaca atgtcctgga aatcagcgaa ccgcgcatct gaagtacatt
      661 tgagcgactg taccagaaca tgaatgaggc gtttggatta ggcgattatt agcagggcta
      721 agcattttac tattattatt ttccggttga gggatataga gctatcgaca acaaccggaa
      781 aaagtttacg tctatattgc tgaaggtaca ggcgtttcca taactatttg ctcgcgtttt
      841 ttactcaaga agaaaatgcc aaatagcaac atcaggcaga caatacccga aattgcgaag
      901 aaaactgttt ggtagcctgc gtggtcaaag agtatcccag tcggcgttga aagcagcaca
      961 atcccaagcg aactggcaat ttgaaaacca atcagaaaga tcgtcgacga caggcgctta
     1021 tcaaagtttg ccacgctgta tttgaagacg gatatgacac aaagtggaac ctcaatggca
     1081 tgtaacaact tcactaatga aataatccag gggttaacga acagcgcgca ggaaaggata
     1141 cgcaacgcca taatcacaac tccgataagt aatgcatttt ttggccctac ccgattcaca
     1201 aagaaaggaa taatcgccat gcacagcgct tcgagtacca cctggaatga gttgagataa
     1261 ccatacaggc gcgttcctac atcgtgtgat tcgaataaac ctgaataaaa gacaggaaaa
     1321 agttgttgat caaaaatgtt atagaaagac cacgtcccca caataaatat gacgaaaacc
     1381 cagaagtttc gatccttgaa aactgcgata aaatcctctt tttttacccc tcccgcatct
     1441 gccgctacgc actggtgatc cttatcttta aaacgcatgt tgatcatcat aaatacagcg
     1501 ccaaatagcg agaccaacca gaagttgata tggggactga tactaaaaaa tatgccggca
     1561 aagaacgcgc caatagcata gccaaaagat ccccaggcgc gcgctgttcc atattcgaaa
     1621 tgaaaatttc gcgccatttt ttcggtgaag ctatcaagca aaccgcatcc cgccagatac
     1681 cccaagccaa aaaatagcgc ccccagaatt agacctacag aaaaattgct ttgcagtaac
     1741 ggttcataaa cgtaaatcat aaacggtccg gtcaagacca ggatgaaact catacaccag
     1801 atgagcggtt tcttcagacc gagtttatcc tgaacgatgc cgtagaacat cataaataga
     1861 atgctggtaa actggttgac cgaataaagt gtacctaatt ccgtccctgt caaccctaga
     1921 tgtcctttca gccaaatagc gtataacgac caccacagcg accaggaaat aaaaaagaga
     1981 aatgagtaac tggatgcaaa acgatagtac gcatttctga atggaatatt cagtgccata
     2041 attacctgcc tgtcgttaaa aaattcacgt cctatttaga gataagagcg acttcgccgt
     2101 ttacttctca ctattccagt tcttgtcgac atggcagcgc tgtcattgcc cctttcgccg
     2161 ttactgcaag cgctccgcaa cgttgagcga gatcgataat tcgtcgcatt tctctctcat
     2221 ctgtagataa tcccgtagag gacagacctg tgagtaaccc ggcaacgaac gcatctcccg
     2281 cccccgtgct atcgacacaa ttcacagaca ttccagcaaa atggtgaact tgtcctcgat
     2341 aacagaccac cacaccttct gcacctttag tcaccaacag catggcgatc tcatactctt
     2401 ttgccagggc gcatatatcc cgatcgttct gtgtttttcc actgataagt cgccattctt
     2461 cttccgagag cttgacgaca tccgccagtt gtagcgcctg ccgcaaacac aagcggagca
     2521 aatgctcgtc ttgccataga tcttcacgaa tattaggatc gaagctgaca aaacctccgg
     2581 catgccggat cgccgtcatc gcagtaaatg cgctggtacg cgaaggctcg gcagacaacg
     2641 caattgaaca gagatgtaac cattcgccat gtcgccagca gggcaagtct gtcgtctcta
     2701 aaaaaagatc ggcactgggg cggaccataa acgtaaatga acgttcccct tgatcgttca
     2761 gatcgacaag caccgtggat gtccggtgcc attcatcttg cttcagatac gtgatatcga
     2821 ctccctcagt tagcagcgtt ctttgcatta acgcaccaaa aggatcatcc cccacccgac
     2881 ctataaaccc acttgttccg cctaatctgg cgattcccac cgcaacgtta gctggcgcgc
     2941 cgccaggaca aggcagtagg cgcccgtctg attctggcaa gagatctacg accgcatccc
     3001 ctaaaaccca tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt
     3061 gataaacctc tttttcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca
     3121 aaacgtcata gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact
     3181 ttatttttat aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa
     3241 atgacgcaat ctcgattgca tgcggcgcaa aacgccctag caaaacttca tgagcaccgg
     3301 ggtaacactt tctatcccca ttttcacctc gcgcctcctg ccgggtggat gaacgatcca
     3361 aacggcctga tctggtttaa cgatcgttat cacgcgtttt atcaacatca tccgatgagc
     3421 gaacactggg ggccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag
     3481 catgagccta ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt
     3541 agtgctgtcg atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat
     3601 ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt
     3661 attcatttcg agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc
     3721 gatcctaaag tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca
     3781 ggcaacacgg ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat
     3841 cgcgtactgg cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc
     3901 agccttggcg atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac
     3961 agttaccgaa atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt
     4021 tttgcacaat ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa
     4081 agctttttag cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg
     4141 ccaatgccct caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca
     4201 gagagcaatg gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag
     4261 catcaatctg tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa
     4321 gcagttgaga ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta
     4381 cagctcggca ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg
     4441 cggtattacc cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac
     4501 acgctcgccc taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg
     4561 gaagcggtga tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat
     4621 gcctcccacg gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat
     4681 atcaggtgga acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac
     4741 gaccttcaat aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg
     4801 tggtcagcgg cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg
     4861 cgacatcctg cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg
     4921 cgcgatcgtt accacatatc agaacatcaa aatctggttt gcccggtttg aagtgggcat
     4981 tgagtaaact tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca
     5041 cctcagccag atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc
     5101 ctgttgccaa cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga
     5161 gcaaatgctg tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg
     5221 ctgggtcatc cgccacacag ttcgccaata caatattttc accatacaga gactcaggca
     5281 gcgtgatatg tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca
     5341 gctgacgtgc cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc
     5401 agccgaactc gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg
     5461 tagccgtgtc ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct
     5521 tacgggcgga aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac
     5581 gcgttgcagg acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca
     5641 ctcccgccag gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac
     5701 gggcactgta gcagaaacag acgtcactgg cgagatccaa cgatattctc aacgccctat
     5761 cacccaacac ggcaatacaa taaaaaataa caataattgc cggataattg tactcaattc
     5821 cgcctctgtt ctcgccaacg agtaaccata taggcaagta agtaaaacat gccaactggc
     5881 atcgccaacc tggagaagaa aatataaata tggatcattg tgctgccgct gtatcccgac
     5941 atcagcccgg aaattattgc gattgctatc ggttcaggtg caattggctg cacgatcgtt
     6001 acggactcgc ttttctggct agtgaagcaa tattgcggcg ctacgctcaa tgaaacattt
     6061 aaatactata cgacagcgac atttatcgct tcagtcgtcg ctctggcggg cacattcctg
     6121 ctgtcattta tcatctaagc gcaaagagac gtactatgga aaacgctaaa atgaactcgc
     6181 tcatcgccca gtatccgttg gtaaaggatc tggttgctct taaagaaacc acctggttta
     6241 atcctggcac gacctcattg gctgaaggtt taccttatgt tggcctgacc gaacaggatg
     6301 ttcaggacgc ccatgcgcgc ttatcccgtt ttgcacccta tctggcaaaa gcatttcctg
     6361 aaactgctgc cactgggggg attattgaat cagaactggt tgccattcca gctatgcaaa
     6421 aacggctgga aaaagaatat cagcaaccga tcagcgggca actgttactg aaaaaagata
     6481 gccatttgcc catttccggc tccataaaag cacgcggcgg gatttatgaa gtcctggcac
     6541 acgcagaaaa actggctctg gaagcggggt tgctgacgct tgatgatgac tacagcaaac
     6601 tgctttctcc ggagtttaaa cagttcttta gccaatacag cattgctgtg ggctcaaccg
     6661 gaaatctggg gttatcaatc ggcattatga gcgcccgcat tggctttaag gtgacagttc
     6721 atatgtctgc tgatgcccgg gcatggaaaa aagcgaaact gcgcagccat ggcgttacgg
     6781 tcgtggaata tgagcaagat tatggtgttg ccgtcgagga aggacgtaaa gcagcgcagt
     6841 ctgacccgaa ctgtttcttt attgatgacg aaaattcccg cacgttgttc cttgggtatt
     6901 ccgtcgctgg ccagcgtctt aaagcgcaat ttgcccagca aggccgtatc gtcgatgctg
     6961 ataaccctct gtttgtctat ctgccgtgtg gtgttggcgg tggtcctggt ggcgtcgcat
     7021 tcgggcttaa actggcgttt ggcgatcatg ttcactgctt ttttgccgaa ccaacgcact
     7081 ccccttgtat gttgttaggc gtccatacag gattacacga tcagatttct gttcaggata
     7141 ttggtatcga caaccttacc gcagcggatg gccttgcagt tggtcgcgca tcaggctttg
     7201 tcgggcgggc aatggagcgt ctgctggatg gcttctatac ccttagcgat caaaccatgt
     7261 atgacatgct tggctggctg gcgcaggaag aaggtattcg tcttgaacct tcggcactgg
     7321 cgggtatggc cggacctcag cgcgtgtgtg catcagtaag ttaccaacag atgcacggtt
     7381 tcagcgcaga acaactgcgt aataccactc atctggtgtg ggcgacggga ggtggaatgg
     7441 tgccggaaga agagatgaat caatatctgg caaaaggccg ttaataacgt ttcaacgcag
     7501 catcgcaatc ctttccctgg gtgagcgatg ctgccgatgg cgcagactta agatccccgg
     7561 tcttacccgc tataaccccc tccttttatc atcacccaac gcctttcgct gtaaacggcg
     7621 gtttcgcaaa ccaaaccaaa accgtaagta agataaaagc aatagccgcc atacggaaaa
     7681 tctcatttgc agaaatagaa agtgactgct gggttatttc attattaatt tcattaagaa
     7741 ctcctgaaag cgaaccatag tatttatcca taatttgtga cgatgaatta aacacggggt
     7801 taaattgatc gatggttgct gtcaactgac tatggtgtaa cgattcgcgt cgtccccaca
     7861 gcgtcattgt caacgacgta ccaactgatc ctgacaaggt acgaaaaaaa ttactcatac
     7921 tcgaggcatt ggcaaattta ttatctggca agcctgaaaa cgaaatcgtt gttaagggta
     7981 aaaagaaaca ggcaacggcg aatccctgga aaaactgtgg caaaatgatg cctgtaaaat
     8041 caatcgttgg cataaatgtc acagaacgcc agtaatagca aaccgcatac atcaaaaaac
     8101 tgaatgtcac taacacccgc atgtctattt tattgccata acgtcctatc aaaggtgaaa
     8161 tcaatagtgg catgatgccg atgggcgcat aagcaagccc ggcccatatc gcattatacc
     8221 ccatcgtttc ctggagtaac tgcggcataa ggacgatcgc tccagagtaa aataaatacg
     8281 cgcaggtgat actcacaata ccaatggtga aattacgaga cttaaacaaa ctgagatcaa
     8341 gaatcggatt ctctgaggtc gactcccaaa tgactaaaga gatcaga
//
LOCUS       DAPGWD010000179         8370 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPGWD010000179 DAPGWD010000000
VERSION     DAPGWD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.180"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(88..294)
                     /locus_tag="RHX88_003881"
     CDS             complement(88..294)
                     /locus_tag="RHX88_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDV2232514.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            607..696
                     /gene="kdpF"
                     /locus_tag="RHX88_003882"
     CDS             607..696
                     /gene="kdpF"
                     /locus_tag="RHX88_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDV2232515.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            696..2369
                     /gene="kdpA"
                     /locus_tag="RHX88_003883"
     CDS             696..2369
                     /gene="kdpA"
                     /locus_tag="RHX88_003883"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDV2232516.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFMQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2392..4440
                     /gene="kdpB"
                     /locus_tag="RHX88_003884"
     CDS             2392..4440
                     /gene="kdpB"
                     /locus_tag="RHX88_003884"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDV2232517.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4449..5021
                     /gene="kdpC"
                     /locus_tag="RHX88_003885"
     CDS             4449..5021
                     /gene="kdpC"
                     /locus_tag="RHX88_003885"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDV2232518.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5014..7698
                     /gene="kdpD"
                     /locus_tag="RHX88_003886"
     CDS             5014..7698
                     /gene="kdpD"
                     /locus_tag="RHX88_003886"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDV2232519.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHLEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7695..>8370
                     /gene="kdpE"
                     /locus_tag="RHX88_003887"
     CDS             7695..>8370
                     /gene="kdpE"
                     /locus_tag="RHX88_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDV2232520.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1725 a         2186 c         2449 g         2010 t
ORIGIN      
        1 gtctaaatat tcctctgaac atcttcctga tacaaaaaac cgccagcgtt tccgctggcg
       61 gttttcaatg tattcaaaac tcagcaatca ataaaaatca ccagttgcct tttcggcctg
      121 atccatccac accggtttat cgctggtttt cgaccagacg cgatgcaggt agctgtaaaa
      181 gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc
      241 ataagtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt ccatactttt
      301 ctcccccttt tgtatctacc cggtgaatgg taccggaaaa atgaatttgt ttatctgatg
      361 aaaatagtac cgccttttgt gtaattttac tactcatccg accacttatt tttgcttatt
      421 gatggtttat ttacattcat cctgtaatta agttacacaa aagttaaatt aatactaaac
      481 attagttaaa tcatggcttt tgccattttt atactttttt tacaccccgc ccgcagattt
      541 ttgcgaaatc tttgcagcca gaattctacc cttccggtat cacttttagg ccactggagg
      601 tgcactgtga gtgcaggcgt gataaccggc gtattgctgg tgtttttatt actgggttat
      661 ctggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga
      721 tcgccacgtt tttactggtg ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc
      781 ggctgattaa tgacattcct cttcccggta caacgggcgt tgagcgcgta ctttttcgcg
      841 cacttggcgt ctctgaccgt gagatgaact ggaagcaata tctttgtgcc attctcggcc
      901 tgaacatgct ggggctggcg gtgctgtttt ttatgttgct cggtcagcac tatctgccgc
      961 ttaatccaca gcagttgccg gggctgtcgt gggatctggc gctgaatacc gccgtcagct
     1021 ttgtcacgaa taccaactgg caatcttaca gcggtgaaac cacgttgagc tatttcagcc
     1081 agatggctgg cttaacggtg caaaactttc tttctgccgc cagcgggatt gcggtgattt
     1141 ttgccctcat ccgtgcgttt acccgccaga gcatgagcac actcgggaat gcctgggtcg
     1201 atctgctacg catcacgtta tgggtgctcg tccctgtggc gttgttgatc gcactgtttt
     1261 ttattcaaca aggtgcgctg caaaactttc agccttatca ggctgtgaat accgttgaag
     1321 gagcgcaaca gctgttaccc atggggcctg tagcttctca ggaagcgatc aagatgctcg
     1381 gtactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa aacccaaccg
     1441 cactgaccaa cttcatgcag atgctggcga tcttcttgat cccaacggcg ctgtgctttg
     1501 cctttggtga agtgacgggc gatcgccgcc aggggcgcat gttgctgtgg gcgatgtcag
     1561 tgatttttgt catctgcgta ggcgtggtga tgtgggcaga agttcagggt aatcctcatc
     1621 tgctggcgct cggcgcagac agcagcatca atatggaagg taaagagagc cgtttcggcg
     1681 tgctggtcag tagcctgttt gcggtcgtga ctacggcggc ttcctgtggc gcggtgattg
     1741 cgatgcatga ttcgtttacc gctctcggtg gcatggtgcc gatgtggctg atgcaaattg
     1801 gtgaagtggt gttcggcggt gtcggttctg gtctttacgg catgatgctg tttgtcctgc
     1861 tggcggtgtt tattgccggg ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa
     1921 tcgacgtacg cgagatgaaa ctgactgcac tggcgattct ggttaccccg acgctggtgc
     1981 tgatgggcgc ggcgctggca atgatgaccg acgccggacg tagcgccatg ctcaaccctg
     2041 gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcatc cgccgctaac aacaacggca
     2101 gcgcctttgc cggattaagc gccaactccc cgttctggaa ctgtttactg gcgttctgca
     2161 tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc aattgccggt tcgctggtga
     2221 gtaaaaagag ccaacccgcc agctccggca cgctgccaac gcacggcccg ctgtttgttg
     2281 gcctgttaat cggcaccgtg ttgctggttg gcgcactgac ctttatccct gccctggcgc
     2341 ttggtccggt ggcggaatat ctctcctgat gatattgagt gagcactgaa tatgagtcgt
     2401 aaacaactgg cgctattcga accaacactt gtcgttcagg cgctgaaaga agcggtgaaa
     2461 aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt
     2521 ctgctgacca cctgtattag catcgcgatg gcaagcggtg cgatgcctgg caatgcgctg
     2581 tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag
     2641 gcgctggcag aaggccgcag taaagcgcag gccaacagtc ttaaaggggt gaaaaaaact
     2701 gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa agttcctgcc
     2761 gatcaactgc gtaaaggtga catcgtgctg gtagaagctg gcgatattat cccctgcgat
     2821 ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca
     2881 ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca ccggcggcac gcgtattctt
     2941 tctgactggc tggtgattga gtgtagcgtt aaccccggtg agacatttct ggatcggatg
     3001 atcgcgatgg tggaaggcgc acagcgacgc aaaacaccga acgagattgc cctgactatc
     3061 ctgctgattg ctctgaccat cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc
     3121 gcgtggggcg gtaatgccgt gagtgtaacg gtactggtgg cgctgctggt ctgtctgatc
     3181 ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag tcggatgctg
     3241 ggcgcgaatg tgattgccac cagcggacgt gcagttgaag cggcaggtga cgttgacgtt
     3301 ctgctactgg ataaaaccgg caccatcaca ctcggtaacc gtcaggcgtc ggagtttatc
     3361 cctgcgcagg gcgtggatga aaaaacgctg gctgacgccg cacaactggc ttcgctggct
     3421 gatgaaacgc cggaaggccg cagtattgtg atcctcgcca agcaacgttt taacctgcgc
     3481 gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt ttactgcgca aagccggatg
     3541 agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc
     3601 catgttgagg ctaacggcgg tcacttccct gccgatgttg atcaaaaagt cgatcaggtt
     3661 gcgcgtcagg gggccacgcc gctggtggtg gtggaaggtt cccgtgtgct gggcgttatt
     3721 gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg
     3781 ggcattaaaa cggtgatgat taccggcgat aaccgtctga ctgccgccgc cattgctgcg
     3841 gaagcgggcg tcgatgattt tctcgccgaa gcgacaccgg aggccaagct ggcattgatt
     3901 cgtcagtatc aggcggaagg ccgtttggtg gcgatgaccg gcgacggcac caacgatgct
     3961 ccggcgctgg cgcaagcaga tgtcgcggtg gcgatgaact ccggcaccca ggcggcgaaa
     4021 gaggcgggca atatggtcga tctcgactct aacccgacca agttgatcga ggtggtgcat
     4081 attggcaaac agatgctgat gacccgtggc tcgctgacca ccttcagcat tgccaacgat
     4141 gtggcgaaat acttcgccat tattccggcg gcgttcgcgg caacgtatcc gcagttaaat
     4201 gcgctgaaca tcatgcgcct gcattcgccc gactccgcca ttctcagtgc ggtcattttc
     4261 aacgccttga ttatcgtctt tttgattcct ctggcgttaa aaggcgtgag ttataaaccg
     4321 cttaccgctt ctgccatgtt gcgccgtaac ttatggattt acggtctggg tgggctgctg
     4381 gtgccgttta ttggtatcaa agtcattgat ttactgctga ccgtttgcgg tctggtgtga
     4441 ggtttaccat gcgtggatta cgtccggcat tatcgacatt tatctttctg ttattgatta
     4501 ctggcggcgt ttacccgctg ctgaccaccg tgctggggca atggtggttt ccctggcagg
     4561 ccaacggttc gttgatccgt gaaggtgata cagtgcgggg ttcggcatta atcgggcaga
     4621 attttaccgg caacggctat tttcatggtc gcccgtcggc aacggcagaa atgccctata
     4681 atccacaggc ttctggcggg agcaatctgg cggtcagtaa ccctgagctg gataaactaa
     4741 tagccgcacg cgttgctgca ttacgggccg ctaacccgga tgccagcgcg agcattccgg
     4801 ttgaactggt gacggcatcg gcaagcgggc tggacaataa tatcaccccg caagcggcgg
     4861 cctggcagat cccacgcgtg gcgaaagcgc gtaatctcag cgttgaacag ctcacgcaac
     4921 tgatcgcaaa atacagccaa caaccgctgg tgaaatatat cggccagccg gtcgtcaaca
     4981 ttgttgaact caatctggcg ctggataaac ttgatgaata acgaaccctt acgtcccgac
     5041 cccgatcgtc tgctggaaca aactgccgcg ccgcatcggg ggaagctgaa agttttcttc
     5101 ggtgcctgtg caggcgtcgg gaagacctgg gcgatgctgg cagaagccca gcgactgcgg
     5161 gcgcaagggc tggatattgt ggttggcgtg gtagaaaccc atgggcggaa agataccgcc
     5221 gccatgcttg aagggctggc tgttctgccg cccaaacgcc aggcgtaccg tgggcggcat
     5281 atcagcgagt ttgatctcga tgccgccctc gcccgccgtc cggcgctgat cttaatggac
     5341 gaactggcgc acagtaatgc gccaggttct cgtcatccta aacgctggca ggatatcgaa
     5401 gaactgctgg aagctggcat tgatgttttc acaaccgtca acgttcagca tctggaaagt
     5461 ctgaatgatg tggtcagcgg cgtcaccgga attcaggtac gggaaaccgt gcccgatcct
     5521 tttttcgatg ccgccgacga cgtagtgctg gtggacttgc ccccggacga tctgcgccag
     5581 cggctgaaag aaggcaaagt ctatattgcc gggcaggcgg agcgcgccat tgaacatttt
     5641 ttccgcaaag gcaatctgat cgccctgcgt gaactggcac tgcgccgtac tgccgatcgc
     5701 gtagatgagc aaatgcgcgc ctggcggggg catcctggtg aagagaaagt gtggcacacg
     5761 cgcgacgcga tccttttatg catcgggcat aacaccggca gtgagaaact ggtccgcgcg
     5821 gcggcgcggt tggcctcgcg cctgggtagc gtctggcacg cggtgtatgt tgaaacccct
     5881 gccctgcatc gcttaccgga aaaaaaacgt cgggcaattc tcagcgcctt acgtctggcg
     5941 caggagctgg gcgcggagac ggctacactt tctgatccag cggaagaaaa agcagtagtg
     6001 cgttatgccc gtgaacataa tctcggcaag attattctcg gtcgcccggc ctcgcgccgc
     6061 tggtggcgtc gggaaacgtt tgctgaccga ctggcgcgca tcgcccccga tctcgatcag
     6121 gtgctggtcg cgcttgatga accacccgcc cgcacgatta acaacgcgcc ggatagccgc
     6181 tcttttaaag acaagtggcg ggtacagatt cagggatgcg tggttgccgc cgcgttatgc
     6241 gccgttatca ctttaattgc catgcagtgg ctgatggcgt ttgatgccgc caacctggtg
     6301 atgctgtatc tgcttggcgt ggtggtggtg gcgctatttt atggacgctg gccttcggtg
     6361 gttgctaccg tcattaatgt ggtgagtttc gatctctttt ttatcgcccc acgcggcacg
     6421 ctcgctgtct ctgatgtgca atatctgctg accttcgcgg tgatgttaac cgtcgggctg
     6481 gtgatcggga accttactgc gggtgtgcgt tatcaggcgc gggtagcccg ttaccgcgag
     6541 caacgcacac ggcacttata tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag
     6601 gacatcgccg ccaccagcga acaatttatt gcctccacgt ttcatgcccg cagtcaggtg
     6661 ttgttgcccg atgacaacgg taaattgcag ccgttaacac atccgcaagg aatgacgcca
     6721 tgggacgatg ccatcgcgca gtggagtttt gataaaggcc tgcctgcggg tgcgggcacc
     6781 gacacgttac ccggcgtgcc gtaccagatt ttgccgctaa aaagcggcga gaaaacctac
     6841 ggactggtgg tggtggaacc ggggaatctg cgtcagttga tgatcccgga gcagcagcgc
     6901 ctgctagaga cgtttacgct gttagtcgcc aatgcgtttg agcggctgac gctgaccgcc
     6961 agtgaagaac aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggcg
     7021 gcgctttcgc atgatttacg cacgccgctt acggtgctgt ttggtcaggc agaaatctta
     7081 acgctcgatc tggcaagcga aggatcgccc cacgcccgcc aggccagcga gattcgtcag
     7141 catgtgctga acactacccg actggtgaat aatctactgg atatggcgcg aattcagtcc
     7201 ggcggcttta atttgaagaa agagtggtta acgctggaag aagtggtcgg tagcgcgctg
     7261 caaatgctgg aaccgggttt atcgtcgccc atcaatcttt ctctgccaga accgctgacc
     7321 ttaatccacg ttgacgggcc actctttgaa cgggtgctga ttaatctgct ggagaacgcg
     7381 gtgaaatatg cgggtgctca ggccgaaatt ggtatcgatg cccaccttga gggcgaaaat
     7441 ctacaactgg atgtctggga taacggcccc ggtcttccgc caggccagga acagacgata
     7501 tttgataagt ttgctcgcgg gaataaagag tcggcggtgc cgggtgtcgg gcttggattg
     7561 gcaatttgtc gggcgattgt ggatgtacac ggcggcacca ttaccgcgtt caaccgaccg
     7621 gaaggtggtg cctgttttcg tgttacactt ccccagcaaa ccgctcctga acttgaagac
     7681 tttcatgagg atatgtgaca aacgttctga ttgttgaaga tgaacaggct attcgtcgct
     7741 ttctgcgcac ggcgctggag ggcgacggga tgcgcgtctt tgaggccgaa actctgcaac
     7801 gcggcttgct ggaagcagca acccgtaagc cagatttgat tattctcgat ctcggcctgc
     7861 ccgatggtga tgggattgag tttatccgcg acctgcgcca gtggagcgcg gtgccggtga
     7921 ttgtgctttc cgcacgcagc gaagagagcg acaaaatcgc cgcgctggat gccggagcgg
     7981 atgattatct gagtaagccg tttggcattg gcgaattgca ggcccgtctg cgcgtcgctt
     8041 tacgccgcca ctccgccacc gccgcgcccg atccgctggt gaaattttcc gatgttaccg
     8101 tcgatttagc cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acgccgattg
     8161 agttccgcct gctggcggtg ctgctgaata atgccgggaa agtgctcacc cagcgccagt
     8221 tactcaatca ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata
     8281 tgggacatct gcgacaaaaa ctggaacagg atcccgctcg cccccgccat ttcattactg
     8341 aaaccggtat tgggtatcgg tttatgccat
//
LOCUS       DAPGWD010000180         8364 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPGWD010000180 DAPGWD010000000
VERSION     DAPGWD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.181"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(60..1652)
                     /locus_tag="RHX88_003888"
     CDS             complement(60..1652)
                     /locus_tag="RHX88_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDV2232521.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="RHX88_003889"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="RHX88_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV2232522.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2849..3967)
                     /locus_tag="RHX88_003890"
     CDS             complement(2849..3967)
                     /locus_tag="RHX88_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDV2232523.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWASLVGYVLLVMIPDW"
     gene            complement(3964..4431)
                     /gene="mntR"
                     /locus_tag="RHX88_003891"
     CDS             complement(3964..4431)
                     /gene="mntR"
                     /locus_tag="RHX88_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDV2232524.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4617..4745
                     /gene="mntS"
                     /locus_tag="RHX88_003892"
     CDS             4617..4745
                     /gene="mntS"
                     /locus_tag="RHX88_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDV2232525.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5017..6600
                     /gene="opgE"
                     /locus_tag="RHX88_003893"
     CDS             5017..6600
                     /gene="opgE"
                     /locus_tag="RHX88_003893"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV2232526.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LTWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6649..7164)
                     /gene="ompX"
                     /locus_tag="RHX88_003894"
     CDS             complement(6649..7164)
                     /gene="ompX"
                     /locus_tag="RHX88_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDV2232527.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7217..7282)
                     /locus_tag="RHX88_003895"
     CDS             complement(7217..7282)
                     /locus_tag="RHX88_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232528.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7517..>8364
                     /gene="rhtA"
                     /locus_tag="RHX88_003896"
     CDS             7517..>8364
                     /gene="rhtA"
                     /locus_tag="RHX88_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDV2232529.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTV"
BASE COUNT         2045 a         2228 c         2011 g         2080 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca gttcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtatttc acttccagat cggccacttt atagccgtct
     1321 tcttcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccag cgtcggctca
     1501 aggtcgccgc cgagaatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg gggctgtatg cactctacat cggtcgcctg tacgctatcc atggcaccaa
     2461 cgctaacttc ggtatcggtc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata atccgctgtc
     2641 taccaccgaa gcccagtttg aaggacagga aattgtgcca attaccctga cgaagagcgt
     2701 gcagacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgatcaaaaa
     2761 tcgttccggg atgccggttc gtctgaatta atcgtcaaaa cgtaaaacaa aggcggactc
     2821 ataatccgcc ttttttattt gccagacctt accagtccgg gatcataacc aacaaaacat
     2881 atcccaccag cgacgcccac aacagcatcg gtattgaata gagatggaaa cgccaccaga
     2941 tgcggcgatc gttcgccata cgtagcgcaa tcaaatttgc cagcgatccg ggtaataacc
     3001 caaagccacc tacatttacc gcccatgcca gtaataaaga cggcggcaca tagttcagca
     3061 acaatatggt actcggcacg ttactgatca cctgcgataa accgattgcc gttaaccata
     3121 acccgggttc agatagatga ctcacgttac ccaacacgcc ttgcaacgct ggaagctggg
     3181 tcagtaaatg gacgtcgata aacatcgcca taaacaccag cagcagcgtc cagtccacac
     3241 tgagcaccac gcgacgtgcc agcagcgcaa agcctgccgc cacaatcacc agtccccaca
     3301 gctcttgttt gaactccagc gccgtcagaa agacgatata cagccccaga caactccaca
     3361 ccagccgcgg tttccactcc ggtgtttgca cccccgtatg gtattgcagc gcctttccag
     3421 ggaaacaaca ccagcacagg agcaggagcg tcagcatcat tgcgccagcc agcggtgcca
     3481 tttgggcaat aaatccggca aacgaaagac cagaacgtcc ccagataaga atattttgcg
     3541 ggttgccaat tggcgtcagt agcgaaccag cgttgactgc cagcgcctca aaaataatca
     3601 gccgattaac cgggatctca cacagtcttt ttagcgtgat agtcagcgga acaacaataa
     3661 acagcgcgac atcgttggtc agaaaggtag aaagcagcgc cgccgccagc accataaaca
     3721 tcgccagccg acgctccgta gcaaagcggc gcaccatttt gcgccccagc acatcaaaat
     3781 aaccgcttaa ctccacacct ttggtcagca gcatcaggcc gcttaaggtg atgatggtgt
     3841 gccagtcgat agcagcaggc caggatttcg gtgcaaaggg cacgaaaaag cttaatccga
     3901 taccaacaag aattaataac tgaaaaaaac gatcgccttg cagcgtgcgt aaaaaaggca
     3961 ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaaa gcgtcgagcg tctcttcact
     4021 aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt tccggactga cgcccaacac
     4081 caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca gcttctctcc
     4141 ttctgccgtt aaaaacacgc cacgccaggg gatcatttca atcagcccca tggttgccag
     4201 ccgcttaagc attttagcca ccgtcggttg cgaaactccc agacgagcag ccatgtccac
     4261 ctgacgagct tcccccactt ccctgatcaa gtcagaaatc agctcaacgt aatcatcaat
     4321 aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct cttccacgtt
     4381 cactaactgc gtcacttttt ttgctgttgg cgtacctgcg cgacgactca ttgtgcttcc
     4441 tcattatggt gacgcataca tagcgttctc atttcgtgcg cacattgtaa accagagttg
     4501 cgaaggtaca aaaaattaac gttttagcaa tagctatata atatagcctg tgctatatct
     4561 gtatgtaatg caatcatccc tcaaggatcg acgggattag caagtcagga ggtcttatga
     4621 atgagttcaa gaggtgtatg cgcgtgttta gtcattctcc ctttaaagta cggttaatgc
     4681 tgctctctat gttgtgcgat atggtcaaca acaaaccgca gcaagataaa ccttccgata
     4741 aatagcggcg tcgcggtacg ccgcttcact cctgctttca tgcaggcata acgcgttttg
     4801 gtctgaaaaa ccccactttt tgtcggattt gcaatcccct tcgcaaaaga tttgttcgtc
     4861 agtagttgac ctgaacggcg gctcgctcta tcttcttgca gccctgcgta tattgcggct
     4921 cgcggatgcg gacccctttc ccctcttcac gcactcttgc aggtattgac ccttgacgcc
     4981 agggtaagca catggcgttt gttacgatag tggcatatga atttaaccct caaagaatcg
     5041 cttgttaccc gtagccgggt atttagcccg tggactgcgt tctacttttt acagtcgcta
     5101 ttaattaacc tcggcttagg ttaccccttc agtttgctct acaccgctgc gtttacggct
     5161 attttgcttt tgctatggcg aacattgcct cgcgtacaaa aagttctggt cggtatcagt
     5221 tcgctggtgg cggcttgtta tttccctttt gctcaggcct acggcgcgcc taacttcaat
     5281 acattgctgg cattgcactc caccaatatg gaagagtcga ccgaaatcct gacgattttt
     5341 ccgtggtaca gctacctggt cggcttattt atttttgcgc tcggcgtaat agcaatcagg
     5401 cgaaaaaaag agaatgaaaa agcgcgctgg aataccttcg acagcctgtg tctggtattc
     5461 agtgtggcga cattttttgt tgctcccgtg caaaacctga cctggggtgg cgtatttaaa
     5521 ctgaaagata ctggctatcc ggtatttcgt tttgctaagg atgtcatcgt caataataac
     5581 gaggtgattg aagagcaaga acggatggca aaactttccg gaatgaaaga tacctggacg
     5641 gtcactgccg ttaagccgaa gtatcagacc tatgtggtgg tgatcggtga aagcgcgcgt
     5701 cgcgatgccc tcggtgcctt tggcggtcac tgggacaata ccccgtttgc cagcagcgtt
     5761 aacggtttga tatttgctga ctacattgcc gccagtggct ccacgcagaa atcgcttggc
     5821 ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc aggataactt tgtcaccctg
     5881 gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc agggtcaaat cggcgaatac
     5941 gataccgcta tcgccagcat cgccaaacga gcagatgaag tgtacttcct gaaagaaggt
     6001 aattttgaag cagataaaaa taccaaagac gaagcgttac tggatatgac cgctcaagtg
     6061 ctggcgcaag agcactcgca accgcagctg attgttctac atctgatggg ctcacatccg
     6121 caggcctgcg acaggacaca aggcaaatac gaaacctttg tgcagtcgaa agaaacgtcg
     6181 tgctatctct ataccatgac gcaaacggac gatttactgc gcaagctgta cgatcagtta
     6241 cgcaacagcg gcagcagctt ctcgctggtt tacttttctg accacggtct ggcctttaaa
     6301 gagcgcggca aagacgtgca ataccttgcc catgatgata agtatcagca aaatttccag
     6361 gtgcctttta tggtcatttc cagcgacgat aaagcgcatc gggtgattaa agcccgccgc
     6421 tcagccaatg acttcttagg ttttttctcc cagtggacgg gaattaaagc gaaggaaatt
     6481 aacatcaaat acccgtttat atctgagaag aaagccgggt cgatatacat caccaacttc
     6541 cagttacaga aggtggatta caaccatctc ggaaccgata ttttcgaccc gaagccttaa
     6601 aaacaaaaat ccgccccgag aggcggattt tttatatcac caaagtgatt agaagcggta
     6661 accaacaccg gcaatccagg tgcctacgtc aacgctacga atacggctct gctcgtaaga
     6721 gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga ccagcaccgt aggagaaacc
     6781 gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg gtctggaatt taccataacc
     6841 cacacccact acaccgtaga tgcttgccca gtcgttaatg cggtaagccg gaccagcagt
     6901 gatgccgtag tactggtttt tgttgtagtc accagagctt gcagtacggc ttttctcggt
     6961 gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct tcttcatagc ggtatttcag
     7021 gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg ctctgtgcgt aaccgccagt
     7081 tacggtagaa gtcgcagcta cggaagtacc tgcggtgaaa gccagaactg cggccagtgc
     7141 tgaaagacat gcaatttttt tcataaccac ctcaaatgtg attcaaataa gtcctaagtt
     7201 ttaaatatat caaaaattaa tgggaaactc ttcgcgattt gtgatgtcta acgggccatt
     7261 tcatgtaaca gaacgtttcc atacaccgct atccatctaa atttaaatca ctttttcaga
     7321 gaactgcgta agtattacgc atgttttccc tgtcattcat ccagattatt cctaatcacc
     7381 agactaatga ttccatcaat cctggcgcat tttagtcaaa acgggggaaa attttttcaa
     7441 caaatgctca accagcattg ggtatatcca gtacactcca cgctttactt aagtctagat
     7501 atttgtggga gaaaggatgc ctggttcatt acgtaaaatg ccggtctggt taccaataat
     7561 catattgctc gttgccatgg cgtctattca gggtggagcc tcgttagcta agtcactttt
     7621 tcctctggtg ggcgcaccgg gtgtcactgc gctgcgtctg gcattaggaa cgctgatcct
     7681 catcgcgttc tttaagccat ggcgactgcg ctttgccaaa gagcaacggt taccgctgtt
     7741 gttttacggc gtttcgctgg gtgggatgaa ttatcttttt tatctttcta ttcagacagt
     7801 accgctgggt attgcggtgg cgctggagtt caccggacca ctggcggtgg cgctgttctc
     7861 ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg gttcttggtc tgtggttcct
     7921 gctaccgctg gggcaagacg tttcccatgt cgatttaacc ggctgtgcgc tggcactggg
     7981 ggccggggct tgttgggcta tttacatttt aagtgggcaa cgcgcaggag cggaacatgg
     8041 ccctgcgacg gtggcaattg gttcgttgat tgcagcgtta attttcgtgc caattggagc
     8101 gcttcaggct ggtgaagcac tctggcactg gtcggttatt ccattgggtc tggctgtcgc
     8161 tattctctcg accgctctgc cttattcgct ggaaatgatt gccctcaccc gtttgccaac
     8221 acggacattt ggtacgctga tgagcatgga accggcgctg gctgccgttt ccgggatgat
     8281 tttcctcgga gaaacactga cacccataca gctgctggcg ctcggcgcta tcatcgccgc
     8341 ttcaatgggg tctacgctga cagt
//
LOCUS       DAPGWD010000181         8358 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPGWD010000181 DAPGWD010000000
VERSION     DAPGWD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.182"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(192..500)
                     /locus_tag="RHX88_003897"
     CDS             complement(192..500)
                     /locus_tag="RHX88_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HDV2232530.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            complement(587..1231)
                     /locus_tag="RHX88_003898"
     CDS             complement(587..1231)
                     /locus_tag="RHX88_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDV2232531.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            complement(1411..2217)
                     /locus_tag="RHX88_003899"
     CDS             complement(1411..2217)
                     /locus_tag="RHX88_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDV2232532.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSAQLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPITPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            complement(2218..2523)
                     /gene="ccdB"
                     /locus_tag="RHX88_003900"
     CDS             complement(2218..2523)
                     /gene="ccdB"
                     /locus_tag="RHX88_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HDV2232533.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(2525..2743)
                     /gene="ccdA"
                     /locus_tag="RHX88_003901"
     CDS             complement(2525..2743)
                     /gene="ccdA"
                     /locus_tag="RHX88_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HDV2232534.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            3301..4227
                     /locus_tag="RHX88_003902"
     CDS             3301..4227
                     /locus_tag="RHX88_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232535.1"
                     /translation="MSEKEHDMTNDGGESVTYTLRNIPADTDRVITDLASHARKPKAT
                     FLREFLEDSFRDVIDSFALKNPLIASLDEELASYLDAKVMEQRYQSHFITRWNQEYQK
                     LLGISTEEELRRLVLNNTPFLQVRADQVLKGWKNIPRGISLTFSLFAEIAGRDRETID
                     QAWKNIFYSQLREKKHRFYQDIEAIRALKKLPALTGDSWTRDGITVRIYRPENYARGA
                     WRVTLSLPENYATQMWNIPFPELEYRLFTADPGYSALISAEPDRWDKAFRFVDGVCEL
                     HLYTNGVEEDHNPTPLGDVAQALINVVEENLL"
     gene            4272..4526
                     /locus_tag="RHX88_003903"
     CDS             4272..4526
                     /locus_tag="RHX88_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232536.1"
                     /translation="MKGRQSRYVTGGESFAEIARLPSGAVVRLCLNTGLEDALREASK
                     SLKSAFTRSGRKCRLSAGTAQGPFTGRRQGVATHLFVSVL"
     gene            5025..5369
                     /locus_tag="RHX88_003904"
     CDS             5025..5369
                     /locus_tag="RHX88_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HDV2232537.1"
                     /translation="MKNLLDTEGTVLFPFQTEIHFIWTIFSTVKRLVIGTRDHICQKQ
                     YWSACLCILLLMAYVGLCAAVVWFVVPWLKAFIVSKFAVSALWVTPVKRGLLWGIGKV
                     VTATCRRFTLLK"
     gene            complement(5545..6546)
                     /locus_tag="RHX88_003905"
     CDS             complement(5545..6546)
                     /locus_tag="RHX88_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HDV2232538.1"
                     /translation="MADPFNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLDNHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQAMETLGLSSGGDTKD
                     IFLRQLVQQVSHIDLLLKKDWYLLETRPERPFYVSDNPVVLKNSNDFGPYGNLGLAVS
                     GIQIYLPLSSTLMLAMYCPSIREQMVRQKQHLQHLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLLMPLTPEHVTHYNSLQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
     gene            6819..7409
                     /locus_tag="RHX88_003906"
     CDS             6819..7409
                     /locus_tag="RHX88_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232539.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVCLEGDPLEVAQWLQACHDAGIEVRVQINESSVP"
     gene            complement(7427..7774)
                     /locus_tag="RHX88_003907"
     CDS             complement(7427..7774)
                     /locus_tag="RHX88_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232540.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVAVLTGGLWGTSWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            7893..8240
                     /locus_tag="RHX88_003908"
     CDS             7893..8240
                     /locus_tag="RHX88_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B immunity protein"
                     /protein_id="HDV2232541.1"
                     /translation="MKLDISVKYLLKSLIPILIILTVFYLGWKDNQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFILCKALSGK"
     gene            complement(8258..>8358)
                     /locus_tag="RHX88_003909"
     CDS             complement(8258..>8358)
                     /locus_tag="RHX88_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colicin-Ib"
                     /protein_id="HDV2232542.1"
                     /translation="PVGILGFAIIMAAVSALVNDKFIEQVNKLIGI"
BASE COUNT         2149 a         2148 c         2105 g         1956 t
ORIGIN      
        1 aacatcgtgg acatcatggt tcaggttaag ggaaaaggaa tgaaatggtg aaactgagag
       61 gaaaggaaag cggtgacgaa tcatgtcacc ataaacagaa agatgtctaa tggggactct
      121 ttaggtacgt gaagaatccc cataacgcat ttaaaagaca cacatcagat acccttaagg
      181 tatccgatag tttattgacc tattgcacca tcacgttcaa gcttctctct tatagcctca
      241 ataatatatg agctaaaatc cagagatgtt ttactggtcg ctttaagttc cgcatgagct
      301 tcaaaatacg acagcggtat ggccttaaga ttcttagcct tcgtcctggc agccacggga
      361 cgacctgaaa cttcctctgc accttcattt ggctgagacg gggctgttgc tgttacggca
      421 gaagaagaag tttgtaatct ggcagaatcc accaggtttt tcagtttttc agcctgtgca
      481 ctaaatttcg ctttgctcat gagataaccc aggatgtttg ttagatactt aaaagatatc
      541 cattaaaagc ccaaaggata ctatatggat acccaataga cacaccttac tgtttgttat
      601 tttcaatcag atgattgatt tcttcgatca gggcaataac ttccttacct cctgccgaat
      661 agcgcccatg aacagactca gtgattccca tcccagtctc aatgacatcc tcaaattctg
      721 cacgagcaga aatgcggctc ttcatcagct tcagatgttt gaacgatggc aggattgcat
      781 caagcttagg gaatttcttc ttgttaggat taactttaca gagatacagg tgagcagtta
      841 tatcggtccc catgatagag ctgatttcgt ccagtactcc atcaaaatgc atcaaaccaa
      901 tacgttcagt cagggagtct tttgaaggaa caataacgca gtctgcaaag gcaatcgcag
      961 tacgggtaag atctgagtca aatcccccac agtcaataag taacgtctta tcgctgttct
     1021 tgtagggagt cataatttcg attaactcat ccactgtttt gggtactttc acggaaattt
     1081 cttttccttc aggtctcagc cccagaatga tagaaagccc ggtatgagta tccagatcaa
     1141 ctgtatcaga cttcatttca ccaacaatgt ttacggatga agttgtctta cccacaccac
     1201 ctttgggatg cgtattagca ataacaggca tagtgtatct ccagaatcca catgtaatac
     1261 ccataaagac cccataagac acctttcgga tatccaagag gtactcaata gatgtttttt
     1321 tactctatac cggtcctgca tgtcaacaga atatgaaata ccgataatat atccatgaga
     1381 tatccgaaag ggatttaaaa aagtcagagc ttatggaatt cttttgagca tagacaccgc
     1441 atcggactca ggcatcgaaa actgcacccg gtgccgtgca gccacatcca gcgcaaatac
     1501 cttcgtgtag acctccgttg agctgatgga cttatgcccc atcaggctct gcaggacctt
     1561 cagcggtata ccggcataca gcatgtgcat cgcataggaa tggcggaacg tatgtggcgt
     1621 gatcgggaca gagaacgtca caccatcagc ggcagcggcg gcaaccgctt ccccaatcca
     1681 ggtcctgacc gttctgtccg tcacttccca gatccgcgct ttctctgtcc tgcctgttcg
     1741 tttattacgc cgttccatgg ggattttcag tgttgccacc atcgtctgca gctggctgac
     1801 gtaccaggag tcggagagcg ggaccagccg gtgagtctgc tgaccggctg gcattcttcc
     1861 tgccgtcctg gcggcttttt cggtccgttg tttcagggtc gcaagctgca caaacggata
     1921 cggaggcgtg agcgaaaaat ccccccgcgt cagcgctagt gcttcattaa tacgcgcgcc
     1981 ggtgttccac agagtggcca gcagcatctt gcggcgcaga tccgggacat aatgaagcag
     2041 ggcactcacc tcgggagcca gcagatattt cggcagttca tcatgaacca tcgacatctg
     2101 gcggagtgcc agagctgccg gataatcaat ggcaacagga agttgtgcag actgtccggc
     2161 agaatacact gccggtacca tggctgcaga ctggctgtgt ataacggagc ctgacattta
     2221 tattccccag aacatcaggt taatggcatt tttgatgtca ttttctcggt ggctgagatc
     2281 agccacttcc tccccgataa cggagaccgg cacactggcc atatcggtgg tcatcatgcg
     2341 ccagctttca tccccgacat gcaccaccgg gtaaagctca cgggagacct tatctgacaa
     2401 cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatgtcact
     2461 ctggacatcc acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg
     2521 catttcacca gtccctgttc tcgtcggcaa aagagccgtt catttcaata aaccgggcga
     2581 cctcagccat cccttcctga ttttctgcct gccagcgttc ggcacgcaga cgacgggctt
     2641 cattctgcag ggtggtgctt accagcccgg agatattgac atcatatgcc ttgagcaact
     2701 gatagctgtc actgtcgacg gtgactgtaa tgcgctgctt cataatgcac ctcatcttac
     2761 atactctctg tacaccatta agtatacacc agtattacgc tataacatgc ctccggcaac
     2821 ggaatattgt tatccagcta ttattaacac caccatcaat aactgacctg aaaagtaatc
     2881 atgacaataa taacctgcaa tgtagtaacc atgataaatt acaggatgac tgtttttcta
     2941 tgatccgatt tttatcggat tcaggatttt ccggaagtga atcaggatca gtgaataagc
     3001 gaagcgatct tttctgccgc tgatgactgg ctcgggaaca cgggaagaaa cacaacatac
     3061 agtgtcatgt cagcggagcg ggacaccaca tcctgtgaaa gcggaccagc gggcggcagc
     3121 gaaggatccg cggatccgta acctggggtt ttcggatctc agaaaaggaa gtgaacagaa
     3181 aacagacagc gaagcatacg aattataaaa acgtatgctt cgcagtattt aaagacactc
     3241 aaaaccagga tagagtataa gtattgcagc aatgccccct cacatgaaaa ggtacgcggg
     3301 atgagtgaaa aagaacatga catgacaaac gacggtggag agagcgtaac gtataccctg
     3361 cggaacatcc ccgctgacac cgaccgggtg atcaccgacc tggcaagcca cgcccgtaag
     3421 ccaaaggcga cgtttctgcg tgagttcctg gaagacagct tccgcgacgt catcgacagc
     3481 tttgccctga agaacccgct catcgcctcc ctggatgagg agctggccag ttatctggac
     3541 gctaaggtca tggagcagcg ctaccagagc cattttatca cccgctggaa tcaggaatac
     3601 cagaagctgc tggggatctc gacggaagag gagctccgcc ggttggtact gaacaacacc
     3661 cccttcctgc aggtccgtgc cgaccaggtg cttaaggggt ggaaaaatat cccgcgcggg
     3721 atctccctga ccttctccct gtttgccgag atcgccggcc gggacaggga aaccatcgac
     3781 caggcctgga aaaatatctt ctactcgcaa ctgagggaaa aaaaacaccg cttttaccaa
     3841 gatatcgagg ccatccgcgc cctgaaaaaa ctgcctgccc tcaccggcga cagctggacg
     3901 cgggatggca tcacggtgcg tatctaccgc ccggaaaatt acgcccgcgg cgcatggcgc
     3961 gttacactga gcctcccgga aaattacgcc acccagatgt ggaacatccc gttcccggag
     4021 cttgaatacc gcctctttac cgccgatccg ggctacagcg ccctgatcag cgccgaaccc
     4081 gacaggtggg acaaggcctt ccgttttgtg gacggggtgt gtgagcttca cctttacacc
     4141 aacggtgtgg aagaagatca caatcccacc ccgctcgggg atgtcgctca ggcgctgatc
     4201 aacgtggtgg aagaaaacct gctgtaacgg acccggatct gcgggcacaa ctgcatcatc
     4261 aggaggatgc aatgaaagga cgacagagcc gctatgttac cggcggagag agtttcgcgg
     4321 agattgcccg tctcccttca ggggcggtgg tgaggctctg tctgaacacc ggtcttgagg
     4381 atgcgctgcg ggaggcctcc aaatcgctca agtcagcctt cacccgttcc gggcgaaaat
     4441 gccggctgtc agcgggtacg gcgcaggggc cgtttaccgg acgccggcag ggcgtggcca
     4501 cacatctctt cgtctcggta ctctgagggg gcagggggca aaaaaagtaa aaatgtattc
     4561 gccaggttgc ccggaggtga aggaaaatag acatacagca gaacgacgga tagcactttt
     4621 tgctaaatgg acatcagtat tactatgcta tagttgcttt aatggataag tgcgccttga
     4681 caaaggcggt gatttctgtt aacattactc tcatagtatt gttccgtccc gctccacccc
     4741 aacaagatcc gtttatttcc cgccagactg gttatcacca ttcaggcccg gatttttttg
     4801 gatttttttc cggggagccc ccggaacgag cttaaaatcg gtatgacaaa caggaggatg
     4861 cgaatgaaca catcataaca gagctgaaag ataaaacatt ctgtacggca ttaacagcgt
     4921 tcacgtgtgt gaggcgccgg gtgccttttg acttaaaaac gaggttattg agatgaccaa
     4981 aatcaagaca gttacttttg taaatactta cccgggaggg ttctatgaaa aacttgttag
     5041 acaccgaggg aacggttcta ttcccattcc agactgaaat ccattttatt tggacgattt
     5101 tctccaccgt taaacgcctg gttatcggaa ccagggacca tatttgccag aagcaatact
     5161 ggagcgcctg tctctgtatt ttgcttctta tggcctatgt gggtctctgt gctgcggtgg
     5221 tctggtttgt agtgccctgg ctgaaggcct ttatagtgtc gaaatttgcg gtttcggcac
     5281 tatgggtcac gccagtaaag cgcggactac tctggggtat cggtaaagtg gttaccgcca
     5341 cttgccgaag atttactctg ctaaagtaag tagccgcaac gctacacgaa ctgatggtga
     5401 atgtcaacag atactcacca tctccttacg gcggtggtcc ctgtgaccac tgccgctttc
     5461 gcgtgggtgc aacacggcaa aactcctctg tacaacaggg gctcccgccg tcattttccg
     5521 gcacaggtga ggccggaatt cggactaaaa cgtaaaccgc gggccagtcc ggtagcgttc
     5581 actatcggcc agcattctct caaccagaga gaaatccttt tcaccgcaga acacgtactg
     5641 ctccgcgaac tccacctgca gggaattgta atgcgtgaca tgctccgggg taagcggcat
     5701 aagaagatag tccgtatacc tccggatatg ttccagtctt tcaaaaggcc gaatgtgacg
     5761 ggggataaga tgcggcgccc tggcgagcag atgttgaaga tgctgctttt gacgaaccat
     5821 ctgttcacgg atggaggggc aatacatggc cagcatcagc gtcgatgaca gggggaggta
     5881 aatctgtatc ccgctaacgg ccagaccaag attaccgtac ggaccgaagt cattgctgtt
     5941 cttaagcact acgggattgt ctgatacgta aaacggacgt tctggcctgg tttccagaag
     6001 gtaccagtcc tttttcagca ggagatcaat atgtgacacc tgctggacga gctgccgcag
     6061 aaaaatatcc ttggtatcgc ccccggacga caggcccagg gtttccatgg cctgctcttt
     6121 agtcgagccg atggcctcca tcttatcggt aagcaccgaa atcatgcctg agatacgctc
     6181 cagttcaccg aaggttcgtg cgcgctgcac ggcaacaaac acggccagcc tgtagcgttc
     6241 atcatctgtc agcctgcgaa tatccctgtg cgccagcagg gctgcaatta ccggtgccgc
     6301 gtggtgctcg taaatcccca gcagtggttc aagactcaga cggtccgggt gattatcgag
     6361 gttgtagaac gtgttgcgga cggtcgcgtc atccggcgtg gtggcatagg cgcgcccggc
     6421 agccttgtca aaggcgtata agcgctgccg cttacccttt cccggcgtac tgaaatgctt
     6481 cagtaaaaaa cggggaaccg tatgctgcct gaccacatcg gtctgcagat tgaatggatc
     6541 ggccataact ttatctccct gaaaagccac gcaagcgcat cggtgcatct gtattgacgg
     6601 catgggcagc tggtttgcag cttagcatta gaaaacagaa ccgttcgata atacctgtgt
     6661 ttaacgccca gtgaattgaa ggcctcagct ctgctgtggt tgtggtatga actggccatt
     6721 catggtgcgc cacagaacaa tcacgcacag gcccgggaac gcatcgatac cgattatcag
     6781 cgcctcaaaa aactcatctg gtcggagggt gacagttaat gactcagagc cgccgaccgt
     6841 cgccactgca gcggagagtg ctgattgtgc tggctgccct tgatgaaaaa cggccgggac
     6901 ccgtgctgac gcgggacata gaacgggtgc tggagcgaag cggggaagcc ccggtctacg
     6961 ggccaaacct gcgcgcctcc tgccgtcgtc tggaggatgc cggctggctt cgtaccctgc
     7021 gcgctccgaa cctgcagctg gcagtggagc tgacggacgc cggccgggcc gtggcacagc
     7081 ctctgctgct ggcagaacag gaccgcctcc gggcagaaca gcgtgcggca gaagtcgtcg
     7141 tactgccgct ggtcccggca gcaggtctgc ccgcagacgg cacttctgcc actgatctgg
     7201 cggtagagct gaacggcata acgtatcagg cctgccgtgg ggattttgtg gtgcgtctcg
     7261 acggcagcac ctgtctgcag ttatggaata aagaaggcag ggtggtatgc ctggagggcg
     7321 atccgctgga agtggcgcag tggctgcagg cctgtcatga cgcaggaata gaggttcgcg
     7381 tacagataaa tgaaagtagc gtcccgtaaa aacatgcctg catggtttac acgtcatccc
     7441 atggtggcag ccggttaact ttcctgcgcc accagtggaa agaggccgtg agcagtccga
     7501 acaaaaaacc acccgtgata cttataataa tggcctcacc tgccaccatt cctgacggcc
     7561 cccagtagat aaaccacatt gcacatcccc aggaagtgcc ccataagccc cccgtcagca
     7621 ccgcgacctg ccagaacggc ataaagggta atggcggaag ccggataccc agccgccaga
     7681 gaatacgcag aagaggaggg gcataattgc tccgccacat ctttttgctg tccatcaggg
     7741 caatggcccg ggcttttttc tgctcaaagg tcacaggagc acctccgttc catgatgtaa
     7801 acgtgacaca gcttattgtt attgattatt attatcatgc tgtgattatg ttatcacgca
     7861 ccggtacacg ataacaataa aggagaaacc agatgaaact ggatatatct gtaaagtatt
     7921 tactgaaaag cctgatacca atcctcatta ttcttacagt tttttatctg ggatggaaag
     7981 ataaccagga aaatgcaaga atgttttatg cgttcatcgg atgcattatc agtgccatta
     8041 cttttccttt ttcaatgagg ataatacaga aaatggtaat aaggtttaca gggaaagaat
     8101 tctggcaaaa agacttcttt acaaatccag ttggcggaag cttaactgca atatttgaat
     8161 tattctgttt cgttatatca gttcctgtgg ttgccattta cttaattttt atactctgca
     8221 aagccctttc aggaaaatga aagggcatca ttatgtgtca gataccaata agtttattga
     8281 cctgctcaat aaacttatca ttaacaagcg cactcacagc cgccataata attgcaaaac
     8341 ccagaatacc tacagggg
//
LOCUS       DAPGWD010000182         8200 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPGWD010000182 DAPGWD010000000
VERSION     DAPGWD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.183"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(5..652)
                     /locus_tag="RHX88_003910"
     CDS             complement(5..652)
                     /locus_tag="RHX88_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV2232543.1"
                     /translation="MLLLIWWLTAAGVWATAGCAWIFQHLHWVTSQKSPGAFVFALAA
                     LIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLYTFSSIATVMGITGGMFAAM
                     ALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSFSVWLASILTVFIWGITAAC
                     EATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVLFRITVTVLEFINGLVW"
     gene            complement(854..1558)
                     /locus_tag="RHX88_003911"
     CDS             complement(854..1558)
                     /locus_tag="RHX88_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV2232544.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1695..2147)
                     /gene="moaE"
                     /locus_tag="RHX88_003912"
     CDS             complement(1695..2147)
                     /gene="moaE"
                     /locus_tag="RHX88_003912"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDV2232545.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(2149..2394)
                     /gene="moaD"
                     /locus_tag="RHX88_003913"
     CDS             complement(2149..2394)
                     /gene="moaD"
                     /locus_tag="RHX88_003913"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDV2232546.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(2387..2872)
                     /gene="moaC"
                     /locus_tag="RHX88_003914"
     CDS             complement(2387..2872)
                     /gene="moaC"
                     /locus_tag="RHX88_003914"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDV2232547.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2875..3387)
                     /gene="moaB"
                     /locus_tag="RHX88_003915"
     CDS             complement(2875..3387)
                     /gene="moaB"
                     /locus_tag="RHX88_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDV2232548.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(3409..4398)
                     /gene="moaA"
                     /locus_tag="RHX88_003916"
     CDS             complement(3409..4398)
                     /gene="moaA"
                     /locus_tag="RHX88_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDV2232549.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(4386..4530)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4795..5703
                     /gene="yvcK"
                     /locus_tag="RHX88_003917"
     CDS             4795..5703
                     /gene="yvcK"
                     /locus_tag="RHX88_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDV2232550.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5895..7916)
                     /gene="uvrB"
                     /locus_tag="RHX88_003918"
     CDS             complement(5895..7916)
                     /gene="uvrB"
                     /locus_tag="RHX88_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDV2232551.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         2033 a         2157 c         2038 g         1972 t
ORIGIN      
        1 cgcactacca cactaaacca ttaataaatt caagaactgt tacagtaatt ctaaagagga
       61 cgcttaaaat actaaaatag agtgttattg ccccaggaac gatacagcga agagaacctg
      121 aaatttcaga ggtgtcggca acacggatca aatcttccag tgttgttgct tcacaggcag
      181 ccgtaatgcc ccagataaag acagtcaaaa tactcgctaa ccacacggaa aaactgctgg
      241 taaggatcag gttaacaaca aaagcaatac tcaaaccaca gaaaatatat gaatataact
      301 gtctgactgg tgggataacc ctattactac aagagcaaat cagcgccatt gcggcaaaca
      361 tccctcccgt aattcccatt accgtcgcta tgctcgaaaa ggtataaagg ctggcgaaga
      421 gaaagccaac aagaaggatt attagcagat agaccagaca tgcgcctgat acattcagtc
      481 ttttcagggc gttgtttaac cagagaccac ctattatcag cgcagccaaa gcgaagacaa
      541 acgcacctgg agacttttgt gacgtaaccc aatgcaaatg ctgaaatatc caggcacagc
      601 ctgccgtagc ccatactcca gctgctgtta accaccaaat gagaagcagc attttgctga
      661 gcgaagcact tttttgctgt aattgagtag cagtggattg tctgttcaaa tgcgttatct
      721 gtccctgaaa ttacgtagtg gacatagtct ggcatttgcc gcgattcagt tctcatgtaa
      781 atgagcggaa tagtctttat aaaatccgaa aaaaatcccg gctccaggcc gggatcagca
      841 ggaagaagag aatttaacgg cggttgccga agatccgcaa caacatcagg aacaggttga
      901 tgaagtccag atacaaggtt aacgcgccaa gaatggaata tttgcgcagg ttcgacgtgt
      961 cgcgggtatc aatctgctca cccatatttt tcagtttctg cgtgtcatac gccgtcaatc
     1021 cgacaaagac aatcacgccg atgtaggtaa ctgcccacat caatgcttcg cttttcaacc
     1081 agaagttgac cagcgatgcc agcacaatgc cgattaacgc cataaacagc atattgccaa
     1141 agccacttaa atcgcgcttc gtggtgtaac cgtacaggct cattgcgccg aacatcccgg
     1201 cagtaacgac gaaagtactg gcgatagaag cagcggtata gacaatgaat atactggaaa
     1261 gcgtaagacc cgtcagcgcc gaataaagca taaagagcat cgtcgttaca cctgcgctca
     1321 gcttttgaat catcgctgat aacacaataa ccaatgctaa ttgcgcgatg atcagaccga
     1381 ttaaaaagac acggttagtg aacaacagct ccatcacggc cgcggaatta gccgcatacc
     1441 aggcaacaaa tgcggtcagc aacaagccaa cggtcatcca gccatagact tgcgccatat
     1501 aagtttgcaa gccagcccgg ggttgtacga ttgaatcaga acgtgggaat ctgtccatga
     1561 cgatctcctg aagatataag gaatatctta aggatactgc aaaatgatga ggctgtgcat
     1621 cgacgcagcg taaacgtatg tactgagcgg tgaaattgcc ggacgcagcg gtgccttatc
     1681 cggctaacaa aaaactacca gcgttttgcc gcctgctgat cgctctcccg ggcttcaacc
     1741 cagcggtcgc cttccggcgt ggcttcgcgc ttccagaacg gtgcgcgggt tttgagataa
     1801 tccataataa actgcccggc ttcaaacgca ctgctgcgat gcgcactggt gacaccgaca
     1861 aaaacgattt catcgcccgg ccataattcc ccgatgcggt gaatcacagt gacgcgcccc
     1921 agcggccagc ggttacgcgc ttcatcaaca atttctgcca gtgctttttc agtcatcccc
     1981 ggatagtgtt cgagggttaa tgctttgacg ctgtcgccca ggttatggtt gcgcacctta
     2041 ccagtaaagg tgactaccgc accgtcttcg tcacgctccg ccagccacgg gtactcttct
     2101 cctacgctga acggctgcgg accaacaaca attttggttt ctgccatctt aacctccggt
     2161 taccggcggg aagaaagcta cttcgtcgcc gtcagtcagc ggatggtcaa aactcaccag
     2221 cgtctggttg acggcagcca gtaatttgcc atcttccagc gccagcgccc agcgatcgct
     2281 ctgcgcagcc atgtgctggc gtaacgcttc aacagttggg aaatccgcag ccacttcggt
     2341 tgcatctgtt cccaccaact cgcgcacctg ggcgaaaaaa agaactttaa tcatccgctt
     2401 ccaccttaaa gtcacccgac ttgccgccgc tcttcgccag caaacgtacc ggaccaatca
     2461 ccatatcttt ttgcaccgct ttgcacatgt cataaatggt cagcgccgcc acggaggccg
     2521 cggttaatgc ttccatttcg acaccggttt tcccggtcag gcggcataag gtttctatac
     2581 gcacccgatt gtgctccggc tcggcctgta aattgacttc aactttgctg agcatcagcg
     2641 gatgacagag cgggatcaga tcccaggtgc gttttgccgc ctgaataccg gcaatacgcg
     2701 cagtggcaaa tacgtcgcct ttgtggtggc gaccatcaat aatcatcgcc agcgtctcgc
     2761 tgcgcatggt gacaaaggct tccgcccgcg cttcacgcac ggtttccgct ttggcggaga
     2821 catccaccat gtgcgcttcg ccagcggcgt tgatatgggt cagttgcgac atacttattt
     2881 cttcaaatgt ggatggaaat tacacggacg cgtacgggca tccagctgcg gcgcgatgat
     2941 attttcccat gcggtacggc acgctttggt cgaacccggc atggcgaaaa tcagcgtttt
     3001 gttggcgacg cccgctaccg cacgagattg caacgtggaa gtgccaatct cttcaaaaga
     3061 caacatccgg aacacttcac caaaaccttc aacttcacgg tcgaacaacg gcagcaatgc
     3121 ttcgggagcc tgatcacctt ccgtcaggcc agtaccaccc gtaatcaata ccacttgtac
     3181 atcgtcgctg gcgatccacg cagatacctg agcgcgaata gcgtagcggt tttctttcac
     3241 aatggcttta tcgacaacgt gatggcccgc ttcttgcgcc gaatcgcgca gatagtgacc
     3301 ggaggtatcg tcttcttcac cgcgacgatt agaaaccgta agaatagcaa tacgggtcgg
     3361 gataaattca gtgcttacct gactcatctg atctctcctt ttgacgtttt agccgccaat
     3421 gtacgataag ttttgcgtaa taccggtgtt gttttgatgc aggaaatggg tctgtttctt
     3481 ctcccgcagc gccgctgaaa tacgcgcttc cagcgcctgt tgctgggtat cgtcttccag
     3541 cagatcgcgc aggttaacgc cgccttcacc aaacaggcag agatggagtt taccaatgga
     3601 ggaaacgcgc aggcggttgc aagtggcgca gaagtctttt tcatacggca tgataaggcc
     3661 aatctctccg gcgtaatctg gatggcaaaa gacttgcgcg ggaccgtcgc tgcgttgacg
     3721 taattggtgg atccagccgc gacgcagtag ctcgtcacgc agaacctgac cagagatgtg
     3781 atgcttacgg aagagctcgc tgccctcgcc cgtttccatc agttcgatga aacgcagctg
     3841 gatagggcga tgctggatcc agttcagaaa ggtgtcgagc tggtgatgat taacatcacg
     3901 catcagcacg gtattgactt tgaccttctc aaaaccggcc tcaaatgcag catcaatccc
     3961 cgccatgacc tggttgaatt tatcctgccc ggtaatagcg tgaaactggc gggcgtcgag
     4021 actgtcgaca ctgacgttaa tgccagtaag tcccgcatcg cgccagttcg ccacatcgcg
     4081 ttccagacgg taaccattgg tggtgaccgc aatctggcgg atagcgtcgt tttcccgcac
     4141 agcggcgatg atatcggtaa agtcgcggcg taaagacggc tcaccgcctg tcaggcgcac
     4201 tttttcggtg cccagactgg cgaaggcgcg cgtaacccgg cgaatttcat cgacggtaag
     4261 aaagccttta ttggtgacgc cgctcggttt gtagccatcc ggcaggcagt aggtgcaacg
     4321 aaagttacac acatcggtaa tcgacaggcg caagtagtaa aacttacgcg caaatgcatc
     4381 agtcagttgt gaagccatgt acacctttcc agatacggga ggcgaagtca tttcttcctt
     4441 cgccctggtg gcaatcttta cgagcattgt cacggccaaa gcaccgtatc agttgaccca
     4501 ggtgcagagg ctagagtgtt tagtggttat gccgatacta gcgtgtaaat gagattttta
     4561 ccatccacac tttcgctatg taatcatgta tatagcgtca tgatcgtgct atttcgctac
     4621 agaataaggg aaaatggcgg ttttactttg atatacatca tcagggaggc atggcgtgca
     4681 tcgtccttta gaggtagaag aaaatagttt gtatcctcaa tattggcagg ttaattgctg
     4741 tttccccgca atttgcgcta ctgtagcgcg gataatctga ctccaggaaa ctatatgcgc
     4801 aatcgtacgc tggctgatct tgatcgtgtc gttgctctcg gcggagggca tggactggga
     4861 cgcgttctct catcactttc gtctttgggt tctcgtttaa cgggtatcgt caccaccacc
     4921 gataatggtg gctcgacggg gcgtattcgc cgttcagaag gcggcattgc ctggggcgat
     4981 atgcgcaact gcctcaacca gctgataacg gaaccgagcg tcgcctccgc gatgtttgaa
     5041 taccgttttg gtggcaatgg cgaactttcc ggtcataatc tcggaaactt gatgttaaag
     5101 gcgctggatc accttagcgt gcggcctctg gaagccatca atttaattcg taatctgctg
     5161 aaagtggata cgcatttgat tccaatgtca gagcatcctg ttgatctgat ggcgattgac
     5221 gatcaggggc atgaagttta cggcgaggtc aatatcgacc agttaactac gccgattcaa
     5281 gagttattgt taacgcctaa tgtacccgca acgcgtgagg cggttcacgc tatcaatgaa
     5341 gcggatctca tcattattgg gcctggcagt ttttatacca gcctgatgcc aattctgctg
     5401 ctgaaggaaa tcgcccaggc attacgccgc acgccagcgc cgatggttta tatcggcaat
     5461 ctggggcgtg agttgagttt acctgcggct aatttgaagc tggaaagcaa gctggcaatt
     5521 atggagcagt atgttggtaa aaaggtcatt gatgcggtca tcgtcgggcc aaaagtggat
     5581 gtctcggcgg tgaaagagcg gattgtgatc caggaggtac tggaggccag cgatattccg
     5641 tatcgtcatg accgccagtt gttacataac gcgctggaaa aggcgttaca ggctttaggt
     5701 taacggaaat ccgaaggaaa attccggctt cctattgaag acaaagtgcg cgttgtttat
     5761 gccggatgcg gcgtgaacgc cttatccggc ctacaaaccg cgcaaattca atatattgcg
     5821 gagaaaatgt aggcctgata agcgtagcac atcaggctgt tttccgtttg tcatcagcct
     5881 tcttcgctat cctgttacga tgcggcaata aacaactcac gcaactgatg caactggtca
     5941 cgaatttgtg ccgcttcttc gaactccaga ttctgcgcgt gttgcatcat cagcccttcc
     6001 agttcatgga ttttctgctg caacgctttc ggcgacatat ccatcggcac attatccggc
     6061 tcaacaatcg ggcgcgattt tcctctgccc ttcgctttgg ttttggcaat gttctgcccc
     6121 agcgccagga tatcgaccac tttcttgttc aagccttgcg gcgtaatgcc gtgttcctcg
     6181 ttgtacttct gctgtttctc acggcgacgt tcggtttcgc caatcgcttt cgccattgat
     6241 ggggtgatct tatcgccgta gagaatcgct ttaccgttaa cgttacgtgc cgcacgacca
     6301 atggtctgga tcaacgaacg ttcggaacgc aggaagcctt ctttgtcagc gtcgaggatc
     6361 gccaccagcg acacttccgg catatccaga ccttcgcgca gtaagttgat ccctaccagc
     6421 acgtcgaact cacccagacg caagtcgcgg ataatctcca tacgttcgac ggtgtcgata
     6481 tctgagtgaa gataacgcac gcgctcgcca tgttcttcga gatattcggt aagatcttcc
     6541 gccatccgct tggtcagtgt ggtgaccagt acgcgttcgt taattgccgc tcgctgacga
     6601 atctccgaaa gaagatcatc aacctgtgtc gccaccggcc gcacttcgat aatcgggtca
     6661 agcaatccgg ttggacgcac cacctgatcc accacatcgc cgccggattt ttccagctcg
     6721 taattacccg gcgtcgccga aacatagatg gtttgcggcg ctaatgcttc gaactcttca
     6781 aacttaagcg gacggttatc cagcgctgat ggcaggcgga agccgtactc caccagtgtc
     6841 tctttacgcg cccggtcacc gcgatacatg ccgccaattt gtggaatggt gacatgagat
     6901 tcatcgacca ccagcagccc atcggcaggg aggtaatcaa acagcgtcgg cggtggctca
     6961 cccggtccac gaccggagag gaagcgcgag tagttttcaa tccccgaaca gtagcccagc
     7021 tcgttcatca tctccagatc aaactgggta cgctgggtca gccgctgctc ttccagcagt
     7081 ttgttgtttt ccaatagcac tttgcgtctg gcggccagct cttctttgat ctcctccatc
     7141 gcctgtacga tgcgctcgcg cggtgtgacg tagtgcgttt tcgggtagat ggtaaaacgt
     7201 ggaatggtgg aaacaatctg cccggtcagc gggtcaaata acgacaatcg ttccacttcc
     7261 tcgtcaaaca gttccacgcg aagtgcaatg tcatccgatt ctgccgggaa gatatcaatc
     7321 acctcgccac gaacgcggaa agtaccacgc tggaatgctt gatcattacg agcgtattgc
     7381 agctccgcca gtcggcgcag aatcgcgcgc tgatcgataa tcataccgac cgtgagatgg
     7441 agcatcatct tgagatataa atcaggatcg cccagaccat aaatcgcgga aacagacgcc
     7501 actacaacca catcacgtcg ctccagcatc gctttggtgg cggataaacg catctgctca
     7561 atatgttcgt taaccgaggc atctttctca atgaaggtgt cggaactcgg tacataggct
     7621 tccggctgat agtagtcgta gtaggagacg aaatactcca ccgcgttttc cgggaagaac
     7681 tctttcattt cgccatacag ctgggccgcc agcgttttgt tgggcgcaag taccatggtt
     7741 gggcgctgaa ggtcagcaat gacattggca atggtgaagg ttttcccgga gccagtcacg
     7801 ccaagtaacg tctggtgcgc caggccatct tccagcccct cttcgagacg tcgaatcgcc
     7861 tctggctgat cgccagaagg tttaaaagcg gaattcagtt tgaacggttt actcatgagt
     7921 cgctacctga aggagttggg cgggcaggta tgtaatttta ctcgtcgtac ttaattatgc
     7981 caacaaatta tactggataa aaaaacagtt catcaccata atatttctgt tacagcgtaa
     8041 actccgctgt caatcatgag caaaatttac tctgtggcga gataaaactc cggccttacc
     8101 gggttatccc caaagcaacg gcttttttaa catttgtcaa gatgagtgat gacagtttta
     8161 tggcaagaga tgcctgttca gtgactgcca ttgcatttat
//
LOCUS       DAPGWD010000183         8178 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPGWD010000183 DAPGWD010000000
VERSION     DAPGWD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.184"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..329
                     /locus_tag="RHX88_003919"
     CDS             <1..329
                     /locus_tag="RHX88_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232552.1"
                     /translation="SPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSSQR
                     LHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTQETLFSSKSHGKYALFPIFTASWRAHR
                     IMNKGV"
     gene            552..1049
                     /gene="ubiC"
                     /locus_tag="RHX88_003920"
     CDS             552..1049
                     /gene="ubiC"
                     /locus_tag="RHX88_003920"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDV2232553.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1062..1934
                     /gene="ubiA"
                     /locus_tag="RHX88_003921"
     CDS             1062..1934
                     /gene="ubiA"
                     /locus_tag="RHX88_003921"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDV2232554.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2089..4512)
                     /gene="plsB"
                     /locus_tag="RHX88_003922"
     CDS             complement(2089..4512)
                     /gene="plsB"
                     /locus_tag="RHX88_003922"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDV2232555.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4683..5051
                     /gene="dgkA"
                     /locus_tag="RHX88_003923"
     CDS             4683..5051
                     /gene="dgkA"
                     /locus_tag="RHX88_003923"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDV2232556.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5161..5769
                     /gene="lexA"
                     /locus_tag="RHX88_003924"
     CDS             5161..5769
                     /gene="lexA"
                     /locus_tag="RHX88_003924"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDV2232557.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5842..7167
                     /gene="dinF"
                     /locus_tag="RHX88_003925"
     CDS             5842..7167
                     /gene="dinF"
                     /locus_tag="RHX88_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDV2232558.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7283..7492
                     /locus_tag="RHX88_003926"
     CDS             7283..7492
                     /locus_tag="RHX88_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDV2232559.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7534..8049)
                     /gene="zur"
                     /locus_tag="RHX88_003927"
     CDS             complement(7534..8049)
                     /gene="zur"
                     /locus_tag="RHX88_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDV2232560.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1840 a         1998 c         2239 g         2101 t
ORIGIN      
        1 tttcacctat atttatagaa actgaagaaa agttaagaac ctttaacact aataaattaa
       61 cacgaaacga taatatattt atcctgttct cctctgtcga tgatgggcct gttatggtgg
      121 taagctccca gcgcttacat gatatgttga atcctacaaa agataccaat tggaattcca
      181 cgtatatcta caaatccaga catgagatgt tgcctgttaa tcttactcag gaaacacttt
      241 tcagctccaa atctcatggt aaatatgcgc ttttccccat ttttactgcg agttggcgag
      301 ctcatcgtat aatgaataag ggtgtttaag taaaggaaaa catcaccgtt cctggcatcc
      361 tgggcggtga tgcccctatg gttgccctct ccagcgcggg catcggcaaa gcgtaaggtt
      421 caacagcgtt ttaccacttc atgcgattgt tgcgtttttg ttgcgtatta gatcacttaa
      481 tttgctttac atctcccgta aacacttttc tgcgatacaa tgcctttacg ttatgtaacg
      541 gagagttcgg catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag
      601 agatccctgc cctggaaccg caactgctcg actggctgtt gctggaggat tccatgacaa
      661 aacgttttga acagcaggga aaaacggtaa gcgtgacgat gatccgcgaa gggtttgtcg
      721 agcagaatga aatccccgaa gaactgccgc tgctgccgaa agagtctcgt tactggttac
      781 gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg
      841 tgtcaacgtt aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg ccgttaggac
      901 gctatctgtt cacatcatcg acattaaccc gggactttat tgagataggc cgtgatgccg
      961 gactgtgggg gcgacgttcc cgcctgcgat taagcggtaa accgctgttg ctaacagaac
     1021 tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc
     1081 agaataagct gctggcgttt catcgcttaa tgcgtacgga taagccaatt ggcgcgttac
     1141 tgctgctctg gccaacatta tgggcgttgt gggtggcgac accgggcgtt cctcagctct
     1201 ggatcctggc ggtatttgtc gcgggtgtct ggctgatgcg cgctgctgga tgtgtggtga
     1261 atgattatgc tgaccgaaag tttgatggtc atgttaagcg cacggcgaac cgaccacttc
     1321 ccagcggcgc ggtaacagag aaagaggcgc gcgcgctgtt tgtagtgctg gtactgattt
     1381 cgtttttact ggtactgacg ctgaatacga tgaccattct gttgtcgatt gccgctctgg
     1441 cgttggcgtg ggtgtacccg tttatgaagc ggtataccca tctcccgcaa gtggtgctgg
     1501 gcgcggcgtt tggctggtcg attccaatgg cttttgccgc tgtgagtgag tcggtgccat
     1561 tgagttgctg gttaatgttc ctcgccaata ttctctgggc ggtggcttac gacacgcagt
     1621 atgcgatggt tgaccgcgat gatgatgtga agattggcat taaatccacg gcaatcctgt
     1681 tcggccaata cgataaattg attattggta ttttgcagat tggcgtactg gcactgatgg
     1741 cgatcatcgg tgagttaaat ggcttaggct ggggatatta ctggtcaatt gtggtggctg
     1801 gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta
     1861 aagcatttat gaataataac tatgttggtc tggtactgtt tttaggactg gcaatgagtt
     1921 actggcattt ctgatgatgt aaaaaagccg gatgatcatc cggctttctt ctgggttgcc
     1981 ttatgcgcgg cgcttctcag gcctacacaa cacatcgcaa tttattgaat ttgcagatta
     2041 tggaaggccg gataaggcgt tttcgccgca tccggcaatt ctctctgatt acccttcgcc
     2101 ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta atcaactccg ccagcaactg
     2161 ataaaccttc atcgtttctg ccggttcggc gtcgccgcta tcgctgatat acccttcatc
     2221 acgcagcgtc agcaccagag aactgaacac cgccttgtcg aagaactccg gcgcgttgat
     2281 gccgtgcagc acggagagac gttgcgcgac ggtgcggctc tctttctcca gcgtaccgcg
     2341 gttgatcgac gggttggcac tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc
     2401 gcgcgcgcct gcggccagca gctgtagggt gcgagaatgc gccgggttga tatgcaactc
     2461 atcatcttgc agggtaatca gcccctgacg ttgcatctca tttgccagcg catcaataac
     2521 gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc gctttcagca ttgggtaaag
     2581 cacattgacg tgctccatca atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc
     2641 cgccatcagc gaaggcagca ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag
     2701 caccgcttgc tcgcgtggca gaatgatgat gtcgccgata gtgtctttct cgacttcaaa
     2761 cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag gaacggtaga
     2821 gtctgtggag taaggtacgt tgcgcatcag atccaggtag cagttgagtt gctcggttaa
     2881 ctgctcgcgg gtgagtgagc gctgacgtga tgccagaagc gcagtacaac acaggttcat
     2941 ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa tattattgac
     3001 cgtcggcgtt aaccaggccg gacgcaccgc ttcgatggga tcgatagatt cacgccagtc
     3061 tggtacgtgc tggttaaggt aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc
     3121 ctgaccgaga ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc
     3181 tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact tccatgacgt gctcataacc
     3241 gatatagatc ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg cctgaatggt
     3301 catcgacagc gtaccggttt tcggatccag caaacgcccc gtgcgggaac gaccgccttc
     3361 cacgaagtac tcgacggaat aaccacggct gaacagttcg ccgagatact cacggaaaac
     3421 ggtggaataa agtttattgc ctttaaaagt acggcgaata aagaacgccc ccagacggcg
     3481 gaaaatcggc ccggccggcc agaagttcag gttgatcccg gcggcgatat gcggcggcac
     3541 cagcccctgg tggtacagca cgtaagaaag tagcaggtag tccatgtgac tgcggtggca
     3601 aggcacatac accagctcat gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg
     3661 gacgttgatg ccctgataaa gtcggttcca ggtgaagccc agaatgcggt cagtcaggcg
     3721 gatcatctcg taagagaaat tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg
     3781 cgctttttca tgggagattt ttttgctgcg cgcttcatct tctaccgctt tggcaatggc
     3841 gcgggaggcg agcagcttat taaacagatc ctgacgagca ggcagacgtg ggcccacagc
     3901 agccagacgt tgacgggcaa agtgcatacg cgccacgcgc gccagtttct gtgcgatagt
     3961 tttatccgtg ccgtgttcat ccgccatacg gcgcagcgaa actgacggcg agaaacgcac
     4021 aaaactgtcg cgaccgagcc acagtacggc gaaaaatttc tgtacgccgt taagcatacg
     4081 cagcggcggg tttacttcgc ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac
     4141 tggcaccatc tgcacatcca gatttgggtt gctacggtgc aaatcgagat agtcgtggaa
     4201 cagcttaata gactcttctt tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat
     4261 gaacacatag cgcggcagta gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa
     4321 gtcatgagcc agacactggg cgcgtaacgt cagcaaatct gctttcgagt tgtacggtaa
     4381 aacgtacata attggacgag aagtatccag ccccagttcc ggggcaggat ctgccggaat
     4441 agacttgctt tttaccagga tgcttaatgg taaattcagt aatttgtagt aaattcgtgg
     4501 ccagccggac ataaacgatg taaagcctct ggttaataat gcaaatgcgc ggcaaggata
     4561 gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac
     4621 ccgaatcatc ggatttaacg gtacactgat attgacgctc ataatgtaaa aaggttcttt
     4681 caatggccaa taataccact ggattcaccc gaattatcaa agctgctggc tattcctgga
     4741 aaggtttacg cgctgcatgg atcaacgaag cggcattccg tcaggaaggc gtagcggtat
     4801 tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc aattacccgc gtgctgctta
     4861 tcagctccgt gatgctggtg atgattgtgg aaattctcaa tagcgccatc gaagcagtgg
     4921 ttgaccgaat tggctctgaa taccatgagc tttccggacg cgctaaagat atgggatccg
     4981 ccgcggtgct gattgccatt atcgtcgccg tgattacctg gtgcattctg ttatggtcgc
     5041 attttggata acccttccag atttcgataa atctctggtt tattgtgcag tttatggttc
     5101 caaaatcgcc ttttgctgta tatactcaca gcataactgt atatacaccc agggggcgga
     5161 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc
     5221 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca
     5281 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc
     5341 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt
     5401 cgtgtggctg ccggtgaacc gcttctggcg caacagcata ttgaaggtca ttatcaggtc
     5461 gatccttcct tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg
     5521 aaagatatcg gcattatgga tggtgacttg ctggcagtgc ataaaactca ggatgtacgt
     5581 aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa
     5641 cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac
     5701 cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg caacggcgac
     5761 tggctgtaac atatctctga gaccgcgatg ccgccaggcg tcgcggtttg tttttcatct
     5821 ctcttcatca ggcttgtctg catggcattc ctcacttcat ctgataaagc actctggcat
     5881 ctcgccttac ccatgatttt ctccaatatc accgttccgt tgctgggact ggtcgatacg
     5941 gcggtaattg gtcatcttga tagcccggtt tatttgggcg gcgtggcggt tggtgcaacg
     6001 gcgaccagct ttctctttat gctgttgctg tttttacgca tgagcaccac cgggctgact
     6061 gcgcaggctt atggtgccaa aaatcctcag gcattagccc gtgcgctggt gcaaccgttg
     6121 ctgttggcgt tgggggctgg ggcgttaatt gcgctgctgc gtacaccgat tatcgatctg
     6181 gcgctgcata ttgttggcgg tagtgaagcg gtcctggaac aggcgcggcg ctttcttgaa
     6241 atccgctggt taagcgctcc ggcgtcgctg gcgaatctgg tattactcgg ttggttactc
     6301 ggcgtgcaat atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat cctcaacatt
     6361 gtgctggatg tctggctggt gatggggctg catatgaacg tgcagggcgc ggcgctggcg
     6421 acggttattg cggaatatgc aacattgctg attggtctgc taatggtgcg taaaatcctc
     6481 aaactacgcg gaatttccgg cgaaatgctg aaaactgcct ggcgaggaaa cttccgtcgc
     6541 ttgctggcgc ttaaccgcga tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc
     6601 gcgatcaccg tacttggtgc gcgactgggg agtgacatta tcgctgttaa cgcggttctg
     6661 atgacgctac tcacctttac cgcctatgcg cttgatggtt ttgcctacgc ggttgaagcg
     6721 cactccggtc aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt atggcgggca
     6781 gcgtgccgcc agtcggggat tgtagcgtta ctgttttcgg tggtttattt gctggcaggg
     6841 gaacacatca ttgcgttgct gacgtcgtta acccagattc agcagctggc tgaccgctat
     6901 cttatctggc aggtgatttt gccgttggtt ggcgtctggt gttatctgct ggacggcatg
     6961 tttataggcg caacgcgtgc cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt
     7021 tttgcgctga cgctccttac gctgccgtgg ctgggtaatc atggtttgtg gctggcatta
     7081 accgtctttc tggcgttgcg cgggctttct ctggcggcta tctggcggcg tcactggcgc
     7141 aacggtacct ggtttgccgc aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc
     7201 aaattgctga ctacacttaa tctcacgttc agaagaaaag tgaacgtact ctcattcaca
     7261 acctaacgat gaggtcttga ttatgaataa agatgaagcc ggcggtaact ggaaacagtt
     7321 taaaggtaaa gtgaaagagc aatggggcaa actgaccgat gatgatatga cgatcattga
     7381 aggtaaacgt gatcaactgg tcggtaaaat ccaggaacgt tatggttatc agaaagatca
     7441 ggcagaaaaa gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt aattaatccc
     7501 tcctgcccga cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac
     7561 agagtgatca tgctggcact gttcaggatg acgacacgct tccacttcca cgcatgccgc
     7621 acagagccca tgtgcttcaa tcacattatg ccgcagggca aaccccattt ttgccgccag
     7681 cgtatgcata atgtcttcca cgccttctgc acactcttct ttcaccgcgc cgcagcgatc
     7741 gcatataaac atggctgacg tatgcgtggg ctggtcgaac agatgacaga gcacataact
     7801 gttggtggat tccaccttat gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata
     7861 aaccgttggc ggcttggctt gcggttcagc ttcgcgcagt aaatcaagca gatcataagc
     7921 gctgatagcg ccatcctgca gactcatcag gcgcaacact tccaggcgct gtggggtcag
     7981 gcgcacatta cgctgcgcgc agattttttc agcctgcgct aataactcct gcgttgtggt
     8041 cttttccatc gggcacctca aagtgggggt taagaatgtc cttactttac catgttccag
     8101 gaaaaacacc gagaaccctt attgttgccg taatgttgat tttctgtttt gtaggtaagg
     8161 tgttgtgttg ccttgtcg
//
LOCUS       DAPGWD010000184         8077 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPGWD010000184 DAPGWD010000000
VERSION     DAPGWD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.185"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            38..298
                     /gene="dinJ"
                     /locus_tag="RHX88_003928"
     CDS             38..298
                     /gene="dinJ"
                     /locus_tag="RHX88_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HDV2232561.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            301..579
                     /gene="yafQ"
                     /locus_tag="RHX88_003929"
     CDS             301..579
                     /gene="yafQ"
                     /locus_tag="RHX88_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HDV2232562.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            735..1475
                     /locus_tag="RHX88_003930"
     CDS             735..1475
                     /locus_tag="RHX88_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDV2232563.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1446..2213)
                     /locus_tag="RHX88_003931"
     CDS             complement(1446..2213)
                     /locus_tag="RHX88_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDV2232564.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2419..2997)
                     /gene="lpcA"
                     /locus_tag="RHX88_003932"
     CDS             complement(2419..2997)
                     /gene="lpcA"
                     /locus_tag="RHX88_003932"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDV2232565.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3237..5681
                     /gene="fadE"
                     /locus_tag="RHX88_003933"
     CDS             3237..5681
                     /gene="fadE"
                     /locus_tag="RHX88_003933"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDV2232566.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5724..6170)
                     /gene="ivy"
                     /locus_tag="RHX88_003934"
     CDS             complement(5724..6170)
                     /gene="ivy"
                     /locus_tag="RHX88_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDV2232567.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6351..7121
                     /gene="yafV"
                     /locus_tag="RHX88_003935"
     CDS             6351..7121
                     /gene="yafV"
                     /locus_tag="RHX88_003935"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDV2232568.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(7163..>8077)
                     /locus_tag="RHX88_003936"
     CDS             complement(7163..>8077)
                     /locus_tag="RHX88_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDV2232569.1"
                     /translation="ARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYD
                     KSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLNMLDIKGKIITTDAMGC
                     QKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREE
                     IRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTV
                     EKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVF
                     KAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         1918 a         2023 c         2100 g         2036 t
ORIGIN      
        1 tcaagctgaa taaatataca gcacaggaga taccccaatg gctgctaacg catttgttcg
       61 cgcccgaatc gatgaagatc tgaagaatca ggcggcggac gtactggccg ggatggggct
      121 gaccatctct gacctggttc gcataaccct cacaaaggtc gcgcgtgaaa aggcattgcc
      181 gtttgattta cgcgagccta atcaattaac cattcaatca atcaaaaaca gcgaagctgg
      241 cgttgatgtt cataaggcca aagacgccga tgatttattt gataaattag gaatttaaat
      301 atgattcaaa gggatattga atactcggga caattttcaa aggatgtaaa acttgcacaa
      361 aagcgtcata aggatatgaa taaattgaaa tatcttatga cgcttcttat caataatgct
      421 ttaccgcttc cagctgttta taaagaccac ccgctgcaag gttcatggaa aggttatcgc
      481 gatgctcatg tcgaaccgga ctggatcctg atttacaaac ttaccgataa acttttacga
      541 tttgagagaa ctggaactca cgcggcgctc tttgggtaaa gcacgctgcc gatgaaattt
      601 gtaaaccctg cgccgacagg cttgcgtaaa actcttttgg tgataatgtg agccttccaa
      661 atccagacta ttccgatttg ggatctattt tgccgtcctg gcgtgtgtaa ccgttttatc
      721 aaggaataag cagtatgcgt aaaatcgcat taattcttgc gatgttgttg atcccgtgcg
      781 tttcgtttgc cggtttgctg ggcagcagta gctcgaccac gccagtgagc aaagagtata
      841 agcagcagtt gatgggatcc cctgtctaca tccagatctt caaggaagaa cgtacgctcg
      901 atctctacgt caaaatgggc gagcaatatc agctgctcga cagttataaa atctgtaaat
      961 attccggcgg cttagggcca aaacagcgtc agggcgattt caaaagcccg gaagggtttt
     1021 atagcgtcca gcgtaatcag ttaaaaccag acagccgtta ctacaaagcg attaatattg
     1081 gtttccccaa tgcctatgac cgtgcgcatg gttatgaagg aaaatacctg atgattcacg
     1141 gcgattgtgt ttccatcggc tgctacgcaa tgaccaatca gggtattgat gagatcttcc
     1201 agttcgttac tggtgcgctg gtgtttggtc agccgagcgt acaggtgagt atttacccgt
     1261 tccgcatgac cgacgccaat atgaagcgcc ataaatattc caactttaag gacttctggg
     1321 agcaactgaa gccgggttac gactactttg agcaaacccg taagccaccg accgtttctg
     1381 tggtcaatgg tcgttacgtg gtcagcaagc cgttaagcca cgaagttgtg cagccacaac
     1441 tggcatcaaa ctacacgctc cccgaggcaa aataagcgcc attcgcctgg cataatcttt
     1501 tgccaggttt cattgcccgt cagcggctgt gttgcaatca ccgtgaccac atcattcggt
     1561 gtggtctgcg agctgaagtc gatttccaca tcctgatcca gcagcgttgc cacgccaaac
     1621 ggcgcgcggc gggtgatcca gtgtaaatta gtcgagcaat acgccatcac atagcgcccg
     1681 tcagaaagca gcatgttgaa aacgcccttc tgccgcagtt catccgccag tgaggcgata
     1741 tatttaaaca ctgccgccat gttgcccggt gtgcgcgggt aacgctgcgt taatttatgc
     1801 aggagccagc agaaggcttt ttcgctatcg gtttcaccga ccgggcggaa gttgccggtt
     1861 tccagtgatt tgtagcccgt cagttgtccg ttatgggcat aagtccagtt acgcccccat
     1921 aactcgcggg taaatgggtg agtattttcc agcgccacct cgccccgatt agcctggcga
     1981 atatgagcca ccaccgaaca ggattttatc gggtagtcct ggacaagttt ggcgatgggg
     2041 gaattaaagc tgggttgtgg atctttaaat gtgcgacagc ctttaccttc gtaaaaggta
     2101 atgccccagc catctttatg tggcccggtt ccaccaccac gctgtacaag tccggtgaaa
     2161 ctaaagcaga tatcggtagg gacgttggcg ctcatcccga gcaattcgca catgggtaac
     2221 ctccacaaca gcgggagcgc atggcccccg gcgaagattg atttaagaat tttcaggtcg
     2281 gatgcggcgt aaacgaatta ttcggcctac ggttcgcgta cagatttgta ggcatgataa
     2341 gacgcgtcag catcgcatca ggcatcagcg cacaagtgcc ggatgcggcg taaacgcctt
     2401 atccggccta cgccagactt acttaaccat ctctttttca attaactgga tcaggatatg
     2461 gatcacttta atgtgaatct cctgaatgcg gtcggcataa ccaaagtgcg gaacgcgaat
     2521 ttcgatatcc gccgtgccag ccattttacc gccgtcttta ccggtcaggg tgatcacttt
     2581 catccccttc tcacgcgccg ctgcgatcgc tttgatcacg tttgcagagt taccggaggt
     2641 ggagatcccc agcagtacat cgccttcgcg acctaccgct tcaacgtagc gggagaaaat
     2701 atcattgaaa ccgaaatcat taccgacgca ggaaatatga ctaacgtcag aaatagcaat
     2761 cgccgggtag cccggacggt tttcacggta gcgaccggtc aactcttcgg caaagtgcat
     2821 agcgtcgcaa tgggaaccgc cgttgccgca ggaaagcact ttgccaccgg ctttaaagct
     2881 gtctgctaac aggaccgccg cgcgctgaat ggcgtgaata ttggcgtcat cttttaaaaa
     2941 gttagccagc gtttccgccg cttcgttcag ttcgttacga ataagatcct ggtacatgag
     3001 gatatccttc agcataaatg taatagacaa aatgcagtgt accggatacc gccaaaagcg
     3061 agaagtacgg gcaggtgcta tgaccaggac tttttgacct gaagtgcgga taaaaacagc
     3121 aacaatgtga gctttgttgt aattatattg taaacacatt gctaaatgtt tttacatcca
     3181 ctacaaccat atcatcacaa gtggtcagac ctcctacaag taaggggctt ttcgttatga
     3241 tgattttgag tattctcgct acggttgtcc tgctcggcgc gttgttctat caccgcgtga
     3301 gcttatttat cagcagtctg attttgctcg cctggacagc cgccctcggc gttgctggtc
     3361 tgtggtcggc gtgggtactg gtgcctctgg ccattatcct cgtgccattt aactttgcgc
     3421 ctatgcgtaa gtcgatgatt tccgcgccgg tatttcgcgg tttccgtaag gtgatgccgc
     3481 cgatgtcgcg cactgagaaa gaagcgattg atgcgggcac cacctggtgg gagggcgact
     3541 tgttccaggg caagccggac tggaaaaagc tgcataacta tccgcagccg cgcctgaccg
     3601 cagaagagca agcgtttctc gacggtccgg tagaagaagc ctgccggatg gcgaatgatt
     3661 tccagattac tcatgagctg gcggatctgc cgccggagtt gtgggcgtac cttaaagagc
     3721 atcgcttctt cgcgatgatt atcaaaaaag agtacggcgg actggagttc tcagcttatg
     3781 cccagtctcg cgtgctgcaa aaactctccg gcgtgagcgg gatcctggct attaccgtcg
     3841 gcgtgccgaa ctcattaggc ccgggcgagc tgctgcaaca ttacggcact gacgagcaga
     3901 aagatcacta tctgccgcgt ctggcgcgtg gtcaggagat cccttgcttt gcactgacaa
     3961 gcccggaagc gggttccgat gcgggcgcga ttccggacac cgggattgtc tgcatgggcg
     4021 aatggcaggg ccagcaggtg ctggggatgc gtctgacctg gaacaaacgc tatattacgc
     4081 tggcaccgat tgcgactgtg cttgggctgg cgtttaaact ctccgacccg gaaaagctgc
     4141 tgggtggcgc agaagattta ggtattacct gtgcgctgat cccgaccacc acgccgggcg
     4201 tggaaattgg tcgtcgccac ttcccgctga acgtaccgtt ccagaacgga ccgacgcgcg
     4261 gtaaagatgt cttcgtaccg atcgattaca tcatcggggg gccgaaaatg gccgggcaag
     4321 gctggcggat gctggtggag tgcctctcgg taggccgcgg catcaccctg ccttccaact
     4381 caaccggcgg cgtgaaatcg gtagcgctgg caacgggcgc gtatgctcac attcgccgtc
     4441 agttcaaaat ctctatcggt aagatggaag ggattgaaga gccgctggcg cgtattgccg
     4501 gtaatgccta cgtgatggat gctgcggcat cgctgattac ctacggcatt atgctcggcg
     4561 aaaaacctgc cgtgctgtcg gctatcgtta agtatcactg tacccaccgc gggcagcagt
     4621 cgattattga tgcgatggat atcactggtg gcaaaggcat tatgctcggg caaagcaact
     4681 tcctggcgcg tgcttaccag ggcgcaccga ttgccatcac cgttgaaggg gctaacattc
     4741 tgacccgcag catgatgatc ttcggacaag gagcgattcg ttgccatccg tacgtgctgg
     4801 aagagatgga agcggcgaag aacaatgacg tcaacgcgtt cgataaactg ttgttcaaac
     4861 atatcggtca cgtcggtagc aacaaagttc gcagcttctg gctgggcctg acgcgcggtt
     4921 taaccagcag cacgccaacc ggcgatgcca ctaaacgcta ctatcagcac ctgaaccgcc
     4981 tgagcgccaa cctcgccctg ctttctgatg tctcgatggc agtgctgggc ggcagcctga
     5041 aacgtcgcga gcgcatctcg gcccgtctgg gggatatttt aagccagctc tacctcgcct
     5101 ctgccgtgct gaagcgttat gacgacgaag gccgtaatga agccgacctg ccgctggtgc
     5161 actggggcgt acaagatgcg ctgtatcagg ctgaacaggc gatggatgat ttactgcaaa
     5221 acttcccgaa ccgcgtggtt gccgggctgc tgaatgtggt gatcttcccg accggacgtc
     5281 attatctggc accttctgac aagctggatc ataaagtggc gaagatttta caagtgccga
     5341 acgccacccg ttcccgcatt ggtcgcggtc agtacctgac gccgagcgag cataatccgg
     5401 ttggcttgct ggaagaggcg ctggtggatg tgattgccgc cgacccaatt catcagcgga
     5461 tctgtaaaga gctgggtaaa aacctgccgt ttacccgtct ggatgaactg gcgcacaacg
     5521 cgctggcgaa ggggctgatt gataaagatg aagccgctat tctggtgaaa gctgaagaaa
     5581 gccgtctgcg cagtattaac gttgatgact ttgatccgga agagctggcg acgaagccgg
     5641 taaagttgcc ggagaaagtg cggaaagttg aagccgcgta aatgaataac ggagccgaaa
     5701 ggctccgttt ctttatccgc taattattta aaattaaagc catccggatg gttttccagg
     5761 ctgccggtca acgccgcgaa caacaccgtt ttaccatcaa tcgaaagcgc atcgttcaca
     5821 ttcagccagg tgagtttctc ttgcgacgtt ttctcatcaa tagtcgagaa cagccccgtc
     5881 atctgattag atttctcgga ccacatcaca gcgatacgtt gcgagccaca gtcatgcggt
     5941 ttgcacgcgc tcatcacctg atacgtctca tctcccaacg ttacggtttg tgcgggagta
     6001 taagtaccgc ctttcatcac ccaggcaggc agcttatgcc cttgtaccat ctgattaaat
     6061 gcagctttgg tggtttcgcc ctttgcaagg ctgctaatgg ttaaatcatc ctgcgccatt
     6121 gcactggtgg cgatgaccag agcggcgact gtcgttattg ccttaaacat cattcctccc
     6181 gagcttatcc tgcccatgtt attaaccttc taaagcctgg cgataacacg ataatttgca
     6241 aatcagatat ttccaatatc agcatttgtt atcaatttat tctgcgctaa ggcaactgcc
     6301 tgataaggtg ttaaagttat aaaacccttc gcataaagga gttgtacacc gtgcctggtt
     6361 tgaagattac gcttttgcag caaccactgg tgtggatgga tggccctgcc aacctgcgtc
     6421 attttgatcg tcaactggaa ggtattaccg ggcgcgatgt gatcgttcta ccggagatgt
     6481 ttaccagcgg ctttgccatg gaagcggcag cttcgtcgct agcacaagat gacgtagtga
     6541 actggatgac agctaaggcg cagcagtgca atgcgctgat tgcaggcagt gttgcattac
     6601 aaacggagtc tggttcggtt aaccgctttt tgctggttga gccgggcggc acggtacatt
     6661 tttatgataa gcgtcatctg ttccgcatgg cagatgagca tctacattat aaagcgggca
     6721 atgcgcgagt gattgtggaa tggcgcggct ggcgtatttt gccgttggtg tgctacgact
     6781 tacgttttcc ggtgtggtcg cgcaatctca acgattatga cctcgccatc tacgttgcca
     6841 actggcctgc tccgcgctct ctgcactggc aggcattgct gacggcccgc gcgattgaga
     6901 atcaggcgta tgtggcggga tgcaatcgcg tcggcagcga tggcaacggc tgccattatc
     6961 gcggtgacag ccgggtgatt aacccgcagg gcgagattat cgctactgcc gacgcgcatc
     7021 aggcaacgcg cattgatgcg gagctgtcga tggtggcatt gcgggaatat agagaaaagt
     7081 ttccggcatg gcaggatgcg gatgagttta ggttgcggtg agtgggggtt gtgtttaagg
     7141 ctgggggaga gtcggggtaa gactacgaaa gcccgctctc cgcaaggact gacgccagat
     7201 agtttctgtc catagctgct tttcgcatct tacgtcttaa ccctgccttg aataccttat
     7261 cattcgtcaa aatattaata gcgatgtgcc gtatccctga aaataattct gcggcgtttc
     7321 ctcttcttat tttgcagtcg tcttcattca ttaccacgtc cagacgccag tgcagcttat
     7381 tctccacgtg ccagtggttt cggattgctg tggcgaactt ttctacggtt aaatcagcag
     7441 aactgatata atatctgacc gtcatttctg gctctttctt ttgttctgct attattgacc
     7501 gaaaggagac tgccacgcat aatttcttca gtcctttcca ttcaaacgtg aaatcaataa
     7561 gttcatcagg gacatcgcaa acaatatgaa gacggatttc ttctctgccg tgactctttt
     7621 cactcattgc gtaactgtca tgctctggat tatttaattc tttcagcgga aatttttcct
     7681 caaaggcttt atttagccgc ccctggtttc cttttaccgc gaataaataa tcacctccct
     7741 gtttttgtat cttctctgca atatctttct ggcaacccat cgcatcagtt gtgatgattt
     7801 ttcctttaat atccagcatg ttaagaagtt ctgggatagc tgtaatctca ttagatttct
     7861 catccgtcct gatctgtccg atgaccagac tgtgcattgt tgagaacgca ctaatgacat
     7921 gaatcgctcc cctgcggcga ctcttgtcat aagagtgccg gagcgttttt ccatcaattg
     7981 cgatgacgtc tttatcatct gaagaatggc agtcacgcat ccagttaata aagcactcat
     8041 gaaattttgc aggactgata caggatacaa ctctggc
//
LOCUS       DAPGWD010000185         7982 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPGWD010000185 DAPGWD010000000
VERSION     DAPGWD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.186"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..811
                     /locus_tag="RHX88_003937"
                     /pseudo
     CDS             1..811
                     /locus_tag="RHX88_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            1174..2211
                     /gene="dusA"
                     /locus_tag="RHX88_003938"
     CDS             1174..2211
                     /gene="dusA"
                     /locus_tag="RHX88_003938"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDV2232570.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2345..2587
                     /gene="pspG"
                     /locus_tag="RHX88_003939"
     CDS             2345..2587
                     /gene="pspG"
                     /locus_tag="RHX88_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDV2232571.1"
                     /translation="MLELLFVFGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2753..3736)
                     /locus_tag="RHX88_003940"
     CDS             complement(2753..3736)
                     /locus_tag="RHX88_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDV2232572.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3819..5234
                     /gene="dnaB"
                     /locus_tag="RHX88_003941"
     CDS             3819..5234
                     /gene="dnaB"
                     /locus_tag="RHX88_003941"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDV2232573.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5287..6366
                     /gene="alr"
                     /locus_tag="RHX88_003942"
     CDS             5287..6366
                     /gene="alr"
                     /locus_tag="RHX88_003942"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDV2232574.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6619..7812
                     /gene="tyrB"
                     /locus_tag="RHX88_003943"
     CDS             6619..7812
                     /gene="tyrB"
                     /locus_tag="RHX88_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDV2232575.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1955 a         1889 c         2065 g         2073 t
ORIGIN      
        1 taattattgc aaaaatagat ttgtttattc aacgaatcac gcataattta tggcatgcac
       61 gtaaaaaaat gtgtttttaa ttgagcaaat aaatgatctc aaaatgtggg tcaataagta
      121 tattgacgat tgcactgatg aggatttaaa cgatcgtgac tttattgcat cagttgttga
      181 ccgggctatt tttcatttcg cgattaatag tatatgtaat cctggggata ataaagatgc
      241 gactcccatt gaacaatgta cttttgatgt agaaactaag aatggccttc cctccacggt
      301 tcagctattt tatgaggaat ctaaggataa tgaaccttta gcgaatatac attttcaagc
      361 aataggttct ggttttttaa cgtttgttaa tgcctgccag gaacatgatg acaacagctt
      421 aaaattattt gcttcgctgt taatttcact ttcatattct agtgcctacg cagatttatc
      481 agaaacagtg tatattaatg aaaataatga gagctacctg aaagctcagt ttgaaaaatt
      541 atctcaacgt gatatgaaga agtacctggg agagatgaag cgtctggctg atgggggaga
      601 aatgaatttt gatggctatc tggataagat gtcacatctg gtgaatgaag gaacgctcga
      661 tcctgatatt ttaagcaaaa tgcgagatgc tgcaccacaa ttaattagct tcgcgaagtc
      721 gtttgaccca acctcaaagg aagagattaa aatacttaca gacacttcta aattaattta
      781 tgatttgttc ggggttaaat cggagaaata atatgtgaag ttcttcgatg gtatggaagg
      841 cattatataa aaggacccaa tatttattgg gttctttttt ctctatcaat gctattagca
      901 gggagatata tcaccagagt ttaatgtgtg attttttatt tatcgtcgaa cctggattgt
      961 ttatcattgg ccttaacaaa gttaacggct aataagatta tttccatcac ttcgtgagag
     1021 cttcatgcct tgagaggatc tcaattttct tttgcaatga gacaggcgct tcctgttgtt
     1081 atggtatagt accccgctat tgagcctcct gaatagtgat gctgaataac ataaccccat
     1141 gatatatcga taaaataatc tctacatttg aaaatgcacg gtaattctga aatgcaaaaa
     1201 atcaaccaaa ccagcgcaat gcctgaaaaa actgacgttc actggagtgg tcggtttagc
     1261 gttgcaccaa tgctcgactg gacggacaga cattgccgct atttcttgcg tctgctttcc
     1321 cgcaatacgt tgctgtatac cgaaatggtg accacagggg cgattattca cggtaaaggt
     1381 gattacctgg cgtacagtga agaagaacat ccggtagcgt tgcaactcgg cggtagcgat
     1441 ccggcggcgc tggcgcagtg tgcaaagctg gcagaagcgc gcggatatga tgagatcaac
     1501 ctgaatgtcg gctgcccgtc tgaccgggtg cagaacggca tgtttggtgc gtgtctgatg
     1561 ggtaatgcgc agctggttgc cgactgcgtg aaagcgatgc gcgatgtggt gtcgattcca
     1621 gtgacggtga aaacgcgtat tggcatcgat gaccaggaca gctatgaatt tctctgcgat
     1681 ttcatcaaca ccgtttccgg caaaggcgag tgtgagatgt ttatcatcca cgcacgtaaa
     1741 gcctggcttt cggggttaag cccgaaagaa aaccgtgaaa tcccgccgct cgattatccg
     1801 cgtgtgtatc aactgaagcg tgactttccg catctgacaa tgtcgattaa cggtggtatc
     1861 aagtcgctgg aagaggccaa agcacacctg caacatatgg atggcgtgat ggtcgggcgc
     1921 gaggcgtatc agaatccggg tattctggcg gcggtagacc gggagatctt tggttcctcg
     1981 gataccgatg ccgatccggt ggcggtagtg cgcgccatgt atccgtacat tgagcgtgaa
     2041 ctcagccagg ggacgtatct cggccatatt acccggcata tgttgggctt gttccagggt
     2101 attcctggcg cgcggcagtg gcggcgttat ttaagtgaaa atgcccataa agcgggtgca
     2161 gacattaatg tgctggaaca cgcgctcaaa ctggtggcgg ataagcgtta acttttcacc
     2221 aaaaagtagt caaattcacc acgccctgcg caccgtcgcg gggcgttttg ctgttaaatc
     2281 aatagattat ttttggcatg attcttgtaa tgccagcaag agatttcata tttgggagag
     2341 catcatgctg gaactacttt ttgtgtttgg cttttttgtc atgctgatgg tcaccggcgt
     2401 ttcgttgctg ggcattatcg ccgcgctggt tgtggcgacg gccattatgt tcctcggcgg
     2461 tatgctggca ttgatgatta agttgctgcc gtggttacta ctggcgattg cggtggtgtg
     2521 ggttattaag gcgattaaag caccaaaagt gccgaaatat cagcgttatg accgctggcg
     2581 ttactaaggg attgtgcgga tgatcacaac ctaaggtttt atccttagaa caaaatagga
     2641 attgataatc aaatctgtca ctattgcgcc tctaacagat tcatcgtgct gtaccctaca
     2701 tacagccgaa ctataaaaag aaagggcttc ccaggtggaa gccctatttc ttttatggaa
     2761 tcagcaggct ggaaccttgc gtcgcccggc tttccagaat ctcatgcgca cgctgcgcat
     2821 ccttcagcgg atatttctgc tgctcggcga catcgacctt aatcacaccg ctggcaatca
     2881 aagagaacag ttcattactg gcctcggtta attcctcccg cgtggtgata tagccttgca
     2941 gggaagggcg tgtcacatac aacgagcctt tttgattgag aatgcctaag ttcacaccgg
     3001 taaccgcacc tgatgagttg ccaaaactga ccattaagcc gcggcgttgc aggcaatcca
     3061 gcgaccgttc ccaggtgtct ctgcccacgg aatcgtacac tacgcgcact ttcttaccgc
     3121 cggtgatctc tttcaatcgc tcgaccagat tctcttcacg atagttaata acctgccacg
     3181 cgccggcttt tagcgcgctc tgcgcttttt gcgcggttcc tacggtgccg ataagtttcg
     3241 cgcccagggc ttttgcccac tggcaggcaa ttaagccaac gccgccagcc gctgcgtgga
     3301 acaggaactg ttcatcgggt ttaatttcat aggttttgcg cagcagataa taaaccgtta
     3361 agcctttcag gaaagatgct gcagcttgtt caaaagaaat tgccgcaggc agaatcgccg
     3421 ctttatccgc attaatgtta tgcacagagc tgtaagcgcc taacgccgac tgcgcataga
     3481 ctacacgatc gcctgcctta atatgcttta caccactgcc gactttactc acgatgcctg
     3541 ccgcttcggt acctaatccg ctgggtagtg atggcggcgg gtaaaggccg ctgcggatat
     3601 acgtgtcgat aaaattgatg ccgatggctt tattttcgac ctggatttca ttctctgccg
     3661 gatcggcagg agtgaactct acggcttgaa gtacttccgg gccaccgtgc ttgtgaaatt
     3721 caattcgtgt tgccatgtgt cctccagaac gtatcgtcag ggtctgcttc atatggtaaa
     3781 gtttcgaccc attctttatc tcggtaactc cattcactat ggcaggaaat aaacccttca
     3841 acaaacagca ggctgaaccc cgcgaacgcg atccacaagt tgccgggctg aaagtgcctc
     3901 cgcactcgat cgaagcggag cagtcggtgt tgggcggttt aatgctggat aacgaacgct
     3961 gggacgatgt agccgagcgt gtggtggcag acgattttta cacccgccca caccgccata
     4021 tctttactga aatggcgcgt ttgcaggaaa gcggtagtcc tatcgatctg attacccttg
     4081 cggaatcgct ggaacgccag gggcaactcg atagcgtcgg tggttttgct tatctggcag
     4141 agctgtcaaa aaatacgcca agtgcggcga acatcagtgc ttatgctgac atcgtgcgtg
     4201 aacgtgccgt tgtccgtgag atgatctcgg ttgcgaatga gattgctgaa gccggttttg
     4261 atccgcaggg gcgtaccagc gaagatctgc tggaccttgc tgaatcccgc gtctttaaaa
     4321 ttgccgaaag tcgtgcaaac aaagacgaag ggccgaagaa catcgccgat gtgctcgacg
     4381 caaccgtggc gcgtattgag cagttgtttc agcagccaca cgatggcgtt accggagtaa
     4441 acaccggtta tgacgatctc aacaaaaaaa ccgctggctt gcagccgtcg gatttgatca
     4501 tcgtcgccgc gcgtccgtcg atgggtaaaa caacatttgc gatgaacctc gtcgaaaacg
     4561 cggcgatgtt gcaggataaa ccagtactta tcttctcgct ggagatgcct tcagaacaga
     4621 ttatgatgcg ttctctggcg tcgctgtcgc gcgttgacca gactaaaatc cgtaccgggc
     4681 agctcgatga tgaagactgg gcgcgcattt ccggcaccat gggtattttg ctcgaaaaac
     4741 gcaatatcta tatcgatgac tcctccggcc tgacgccaac ggaagtgcgt tcccgcgcac
     4801 gccgtattgc ccgtgaacac ggcggcatcg ggcttatcat gatcgactac ctgcaactga
     4861 tgcgcgtacc ggcgctttcc gataaccgta cgctggaaat tgcagaaatc tcccgctcgc
     4921 tgaaagcact ggcgaaagaa ctgaacgtgc cggtggtggc gctgtcccag ttgaaccgtt
     4981 ctctggaaca acgtgccgac aaacgcccgg tcaactccga cctgcgtgaa tctggctcta
     5041 tcgagcagga tgcggacttg atcatgttta tctatcgtga tgaggtgtat cacgaaaaca
     5101 gtgatttaaa aggcatcgcg gaaattatta tcggtaaaca acgtaacggc ccaatcggga
     5161 cggtacgcct gacctttaac ggtcaatggt cgcgcttcga caactatgcg gggccgcagt
     5221 acgacgacga ataataatta ttttatgaat taggtaatta aagcaaacac ttatcaagga
     5281 acacaaatgc aagcggcaac tgttgtgatt aaccgccgcg ctctgcgaca caacctgcaa
     5341 cgtcttcgtg aactggcccc tgccagtaaa atggttgcgg tggtgaaagc gaacgcttat
     5401 ggtcacggtc ttcttgagac cgcgcgaacg ctccccgatg ctgacgcctt tggcgtagcc
     5461 cgtctcgaag aagctctgcg actgcgtgcg gggggaatca ccaaacctgt actgttactc
     5521 gaaggctttt ttgatgccag agatctgccg acgatttctg cgcaacattt tcataccgcc
     5581 gtgcataacg aagaacagct ggctgcgctg gaagaggcta gcctggacga gccggttacc
     5641 gtctggatga aactcgatac cggtatgcac cgtctgggcg taaggccgga acaggctgag
     5701 gcgttttatc atcgcctgac ccagtgcaaa aacgttcgtc agccggtgaa tatcgtcagc
     5761 cattttgcgc gcgcggatga accaaaatgt ggcgcaaccg agaaacaact cgctatcttt
     5821 aatacctttt gcgaaggcaa acctggtcaa cgttccattg ccgcatcggg tggcattctg
     5881 ctgtggccac agtcgcattt tgactgggtg cgcccgggca tcattcttta tggcgtctcg
     5941 ccgctggaag atcgctccac cggtgccgat tttggctgtc agccagtgat gtcactaacc
     6001 tccagcctga ttgccgtgcg tgagcataaa gccggagaac ctgttggtta tggtggaacc
     6061 tgggtaagcg aacgtgatac ccgtcttggc gtagtcgcga tgggctatgg cgatggttat
     6121 ccgcgcgccg cgccgtccgg tacgccagtg ctggtgaacg gtcgcgaagt accgattgtc
     6181 gggcgcgtgg cgatggatat gatctgcgta gacttaggtc cacaagcgca ggacaaagcc
     6241 ggggatccgg tcattttatg gggcgaaggt ttgcccgtag aacgtatcgc tgaaatgacg
     6301 aaagtaagcg cttacgaact tattacgcgc ctgacttcaa gggtcgcgat gaaatacgtg
     6361 gattaatcgt tctgtaatat ttgattgtct gtgccggatg cggcgtgaat gccttatccg
     6421 gccaataaaa tcctaaaaat tcaataagtt gatgttcttt catgctctta taaaggtcgt
     6481 gcctctggcg gatgtacgtt tgtcatgagt ctcactctgt tgctaattgc cgttcgctcc
     6541 tgaacatcca ctcgatcttc gccttcttcc ggtttattgt gttttaacca cctgcccgta
     6601 aacctggaga accatcgcgt gtttcaaaaa gttgacgcct acgctggcga cccgattctt
     6661 acgcttatgg agcgttttaa agaagaccct cgcagcgaca aagtgaattt aagtatcggt
     6721 ctgtactaca acgaagacgg aattattcca caactgcaag ccgtggcgga ggcggaagcg
     6781 cgcctgaatg cgcagcctca tggcgcttcg ctttatttac cgatggaagg gcttaacagc
     6841 tatcgccatg ccattgcgcc gctgctgttt ggtgcggacc atccagtact gcaacaacag
     6901 cgcgtagcaa ccattcaaac ccttggcggc tcaggggcat tgaaagtggg cgcggatttc
     6961 ctgaaacgct acttcccgga atcaggcgtc tgggtcagcg atcctacctg ggaaaaccac
     7021 gtagcaatat tcgccggggc tggattcgaa gtgagtactt acccctggta tgacgaagcg
     7081 actaacggcg tgcgctttaa tgacctgttg gcgacgctga aaacattacc tgcccgcagt
     7141 attgtgttgc tgcatccatg ttgccacaac ccaacgggtg ccgatctcac taatgaccag
     7201 tgggatgcgg tgattgaaat tctcaaagcc cgcgagctta ttccattcct cgatattgcc
     7261 tatcaaggat ttggtgccgg tatggaagag gatgcctacg ctattcgcgc cattgccagc
     7321 gctggattac ccgctctggt gagcaattcg ttctcgaaaa ttttctccct ttacggcgag
     7381 cgcgtcggcg gactttctgt tatgtgtgaa gatgccgaag ccgctggccg cgtactgggg
     7441 caattgaaag caacagttcg ccgcaactac tccagcccgc cgaattttgg tgcgcaggtg
     7501 gtggctgcag tgctgaatga cgaggcattg aaagccagct ggctggcgga agtagaagag
     7561 atgcgtactc gcattctggc aatgcgtcag gaactggtga aggtattaag cacagagatg
     7621 ccagaacgca atttcgatta tctgcttaat cagcgcggca tgttcagtta taccggttta
     7681 agtgccgctc aggttgaccg actacgtgaa gaatttggtg tctatctcat cgccagcggt
     7741 cgcatgtgtg tcgccgggtt aaatacggca aatgtacaac gtgtggcaaa ggcgtttgct
     7801 gcggtgatgt aatgcaggaa agcaggctgg agctacccag cctgcagtga aattaaactg
     7861 tcgtcgcttt cactctttct ttatagatga tttttttgat gccatcgttc tacgtgagag
     7921 ataataaacg ttattagttc ttttattgtt aagcttatcc taattatctg gaattcctta
     7981 tc
//
LOCUS       DAPGWD010000186         7974 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPGWD010000186 DAPGWD010000000
VERSION     DAPGWD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.187"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(45..121)
                     /locus_tag="RHX88_003944"
     tRNA            complement(45..121)
                     /locus_tag="RHX88_003944"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85..87),aa:Arg,seq:acg)
     gene            complement(320..396)
                     /locus_tag="RHX88_003945"
     tRNA            complement(320..396)
                     /locus_tag="RHX88_003945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(360..362),aa:Arg,seq:acg)
     gene            complement(594..670)
                     /locus_tag="RHX88_003946"
     tRNA            complement(594..670)
                     /locus_tag="RHX88_003946"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(634..636),aa:Arg,seq:acg)
     gene            complement(674..766)
                     /locus_tag="RHX88_003947"
     tRNA            complement(674..766)
                     /locus_tag="RHX88_003947"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Ser,seq:gct)
     gene            complement(1082..1267)
                     /gene="csrA"
                     /locus_tag="RHX88_003948"
     CDS             complement(1082..1267)
                     /gene="csrA"
                     /locus_tag="RHX88_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDV2232576.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1502..4132)
                     /gene="alaS"
                     /locus_tag="RHX88_003949"
     CDS             complement(1502..4132)
                     /gene="alaS"
                     /locus_tag="RHX88_003949"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDV2232577.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4260..4760)
                     /gene="recX"
                     /locus_tag="RHX88_003950"
     CDS             complement(4260..4760)
                     /gene="recX"
                     /locus_tag="RHX88_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDV2232578.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(5003..6064)
                     /gene="recA"
                     /locus_tag="RHX88_003951"
     CDS             complement(5003..6064)
                     /gene="recA"
                     /locus_tag="RHX88_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDV2232579.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6144..6641)
                     /gene="pncC"
                     /locus_tag="RHX88_003952"
     CDS             complement(6144..6641)
                     /gene="pncC"
                     /locus_tag="RHX88_003952"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDV2232580.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6786..7871)
                     /gene="mltB"
                     /locus_tag="RHX88_003953"
     CDS             complement(6786..7871)
                     /gene="mltB"
                     /locus_tag="RHX88_003953"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDV2232581.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1846 a         2236 c         1936 g         1956 t
ORIGIN      
        1 cgctacctct tcgatacctt cattgctgaa gatttcggag aatatggtgc atccgggagg
       61 attcgaacct ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggatg
      121 catcgggaaa atttatttta ctgctgatat tgataccgct actaaagcca tatcaagtaa
      181 gagatggtgc atccgggagg attactcgcc ttgcggctcg cccttcgggc cgttgctaaa
      241 gcaacgttat cctccctggt gcttgcggtt agtaccgcca cctcttcgat accttcatcg
      301 ctgaagatta cggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
      361 gtagccgagt actctatcca gctgagctac ggatgcatcg ggaaacttac tttactgcag
      421 attttttgat accgctacta aagccgtatc aagtaagaga tggtgcatcc gggaggatta
      481 ctcgccttgc ggctcgccct tcgggccgtt gctaaagcaa cgttatcctc cctggtgcct
      541 gcggttatac cgccacctct tccatacctt catcgctgaa agtacgtaga atatggtgca
      601 tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag
      661 ctacggatgc aaatggcggt gaggcgggga ttcgaacccc ggatgcagct tttgaccgca
      721 tactccctta gcaggggagc gccttcagcc tctcggccac ctcaccacac gcctcttacg
      781 agtgcttcga agaacttgtt tatcgctcat cgtcgctgcg tggcgcacat attactttct
      841 gagacttata agtcaaacaa tttttcctac acttttatcg tttgcacact tcacgttcaa
      901 ttagtctgta aaaacgtcaa aaagagtgtt ttatcaacag aaaaatggag gtctgacaga
      961 tagtagtaat gcaaaaaaat ggagacttaa gttgaatgaa cgggagtaaa gcgaaaagac
     1021 taaagagtga aggagaaatt ttgagggtgc gtctcaccga taaagatgag acgcggaaag
     1081 attagtaact ggactgctgg gatttttcag cctggatacg ctggtagatc tcttcacggt
     1141 gaacagaaac ttccttcggg gcatttacgc caatacgtac ctggttgccc tttaccccta
     1201 aaactgtcac ggtgacctca tccccaatca tgagggtctc accaactcga cgagtcagaa
     1261 tcagcattct ttgctccttg aaagattaaa agagtcgggt ctctctgtat cccggcatta
     1321 tccatcatat aacgccaaaa agtaagcaat gacaaacaca ttacatgtaa gcagtcatgg
     1381 cattacattc tgttaaacct aagtttagcc gatatacaca acttcaacct gactttatcg
     1441 ttgtcgatag cgttaatgcg aatgccgtga agcgagtcca cggcattgcc tgacgcttat
     1501 attattgcaa tttcgcgctg acccagcctt tcacactggc taacgctgca ggtaaggccg
     1561 cagcatccgt accaccggct tgcgccatgt caggacgtcc accacccttg ccgcccacct
     1621 gctgagcgac cataccaatc agttcccctg ctttcacacg atctgtgacg tccttagata
     1681 cgcctgcaat cagagaaacc ttaccttcgg ctaccgttgc cagcacgata attgtcgacc
     1741 ccagctgatt ttttaaatcg tcaaccatgg tacgcaacat tttcggctca acaccgctaa
     1801 gctcgctaac caacagctta acaccattaa catcaattgc cttactggaa agatttgcgc
     1861 tctcctgtgc ggcagcttgt tctttaagct gttgtagttc tttttctaac tgacgcgtac
     1921 gttccagtac tgagcgcact ttatccgcca gattattgct atcgcctttc agcagatgcg
     1981 cgacttcgct taagcgatcg ctgtctgcat gaacggtggt gatagcgcct tctccggtta
     2041 ccgcttcgat acgacgaacg cctgcagcag tacccgattc agagatgatg cggaacagac
     2101 caatatcacc agtgcggctg gcgtgagtac cgccacacag ctcggtggaa aaatcgccca
     2161 tgctcagcac gcgtacacgc tcatcatact tctcgccgaa cagcgccatc gcacctttcg
     2221 ctttcgccgc ttcgagatcc atgatgttgg tttcgatcgg caaattgcga cgaatctgtg
     2281 cgttcaccag gtcttcgacc gcacgaatct cttccggttt catcgcttcg ttgtgtgaga
     2341 agtcgaagcg cagcaccttg tcgttaacca gtgaaccttt ctgcgataca tgagtcccca
     2401 gaacctggcg cagcgcagcg tgcatcaggt gcgttgcgga gtgattcaga cgaatacggg
     2461 cgcgacgagc ctcatcaaca tcagcctgca cagcgtcgcc cactttcaga gaacccgcag
     2521 caagtttacc gatgtgacca atcgcctggc cgtatttctg cgtatcttcc accgcgaagg
     2581 agaagttagc gcctttcagt tcgcctttat caccaacctg accgccggat tccgcataga
     2641 atggtgtttg atccagcacg accacagctt cctggcctgc attgatggca tcaaccgctt
     2701 taccatcaac aaacagcgcg gtcactttgc cgttcagttc cagatggtca tagcctttaa
     2761 attcagatgc actatcaaca cggatcattg cgttgtaatc ggcaccaaag ccgctggctt
     2821 cgcgcgcgcg acgacgctgc tcttccattg ctgcttcaaa accagcttcg tcaactttga
     2881 tgttgcgctc acgacaaaca tcagccgtca ggtcaaccgg gaagccatag gtgtcgtaca
     2941 gacggaaagc cgtttcacca tccagcgtat caccagaaag ttttgccagc tcttcatcca
     3001 gcaacgccag accgcgctcc agagtacggg caaactgctc ttcttcagtc ttcagcacct
     3061 gctcaacctg cgcctgctgg cgtttcaggt cttcacccgc agagcccata acgtcgatca
     3121 gcggaccaac cagtttgtag aagaaggttt ctttcgcgcc gagcatattg ccgtgacgca
     3181 ctgcgcgacg aatgatacga cgcagtacat aaccacggtt ttcattggac ggcattacgc
     3241 catccgcgat caggaacgca caagaacgaa tgtggtcagc gattacgcgc agcgatttat
     3301 tgctcagatc ggtcgcgcca gtgactttcg ctaccgcctg gatcaacgtg cggaacaggt
     3361 cgatgtcata gttagagtta acgtgttgca gcaccgcagc aatacgctcc agacccatac
     3421 cggtatctac agacggcttc ggcagcggtt ccatcgtgcc atctgcctgg cggttgaact
     3481 gcatgaagac gatgttccag atctcaatgt agcggtcgcc gtcttcttcc ggacttcccg
     3541 gagggccacc ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac gggccgcacg
     3601 gaccagtgtc acccatctgc cagaagttgt cagatgcgta tggcgcacct ttgttatcgc
     3661 cgatgcgaat aatacgttcg cgcgggatcc cgacttcttt ttcccagatt tcgtaggctt
     3721 cgtcgtcgct ttcatagacg gtaacccaca gacgctcttt cggcagggca aaccattttt
     3781 cgctggtcag cagttcccat gcaaactgaa tggcatcgtg tttgaaatag tcgccgaagc
     3841 tgaagttgcc cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg acgttttcca
     3901 ggtcgttgtg tttaccaccc gcacgcacgc agcgttggga agtggtagcg cgggaataat
     3961 tacgcttgtc gagcccaagg aacacatcct tgaactggtt catcccggcg ttggtaaaca
     4021 acaaagttgg gtcgttatgg ggtaccaggg agctgctggc aactacctga tgtcccttac
     4081 tatggaaaaa gtcgagaaac gcctgacgga tctcagcggt gctcttgctc ataattatcc
     4141 tgaaatcaag ctaacgaaat atcgccacca gctccagcgt gtcttaaccg ccgggctggt
     4201 aactgaaaag tgggaataag ataagttttc ttgactggga agtaaaatac cgtatgcgtt
     4261 cagtcggcaa aatttcgcca aatatcctgg atatcttcca tcagatagcc acgatagagc
     4321 agaaaacgct ggatcttaac tttttctgaa aagacagttg gcaaaggttc accatatttt
     4381 cgcgtcgcct gatcgcgcgc cagtgcgcac cagtcgatgt cacattcacg catcgctttt
     4441 tctgtcgctt cgcgggaaat acctttctga ttcagttcct gacgaatacg tgcaggtcca
     4501 taacctttgc ggctacggct ggcgataaag cgcgcaacaa atcggctgtc atcgagatag
     4561 ccatgttcat ggcaccaggc aataacgcgc tcgtaatctt ctgccgtagc atcaatctct
     4621 tctgggccat ttttgcccat aatcggtgcc gcgagtttac gtcgcagttc ttgctcactg
     4681 tgatcgcgca ccgccagaat gcgtaccgca cgatccaaca ggcgagcata tgccgggcga
     4741 cgggacgttg attctgtcat ggcatatcct tacaacttaa aaaagcaaaa gggccgcaga
     4801 tgcgacccag gttttgctga taaacagatt taatagtcaa tgagtgtgca gaatgttccc
     4861 gcaggcccaa ttcgttagag gatcgaggtg agagtaattt ttcacgccag tgctggcctg
     4921 ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccgtgc acacattatc
     4981 tattatcatc aataacgaac gattaaaaat cttcgttagt ttctgctacg ccttcgctgt
     5041 catctacaga gaaatccggc gttgagttcg ggttgctcag cagcaactca cgtactttct
     5101 tctcgatctc tttcgcggtt tccgggttat ctttcagcca ggcagtcgca ttcgctttac
     5161 cctgaccgat cttctcacct ttgtagctgt accacgcgcc tgctttctcg atcagcttct
     5221 cttttacgcc caggtcaacc agttcgccgt agaagttgat accttcgccg tagaggatct
     5281 ggaattcagc ctgtttaaac ggcgcagcga ttttgttctt caccactttc acgcgggttt
     5341 cgctacccac cacgttttcg ccctctttca ccgcgccgat acgacggatg tcgagacgaa
     5401 cagaggcgta gaatttcagc gcgttaccac cggtagtggt ttccgggtta ccgaacatca
     5461 caccaatttt catacggatc tggttgatga agatcagcag cgtgttggac tgcttcaggt
     5521 tacccgccag cttacgcatc gcctggctca tcatacgtgc cgcaaggccc atgtgagagt
     5581 cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc cgccacggag tcaacgacga
     5641 taacgtctac tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc tgctcgccgg
     5701 tgtccggctg ggagcacagc aggttgtcga tatcgacgcc cagtttacgt gcgtagattg
     5761 ggtccagcgc gtgttcagca tcgataaacg cacaggtttt accttcacgc tgcgctgcgg
     5821 cgatcacctg cagcgtcagc gtggttttac cggaagattc cggtccgtag atttcgacga
     5881 tacggcccat cggcagacca cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag
     5941 agatggtttc cacatccatg gaacggtctt cacccaggcg catgatggag cctttaccaa
     6001 attgtttctc aatctggccc agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag
     6061 ccatttttac tcctgtcatg ccgggtaata ccggatagtc aatatgttct gttgaagcaa
     6121 ttatactgta tgctcataca gtatcaagtg ttttgtagaa attgttgcca caaggtctgc
     6181 aatgcatacg cagtagcctg acgacgcacc gcatcacggt cgccgctgaa gcattcccgc
     6241 cgggtaatgc cttcaccgcg ggcagtggca aaagcaaacc agacggtgcc gacaggcttc
     6301 tcttcactgc cgccatccgg cccggcgata ccactaatag acacggcata atcagcacga
     6361 gccgctttca gtgcgcctat cgccatttcc accacgacgg gttcactcac cgcgccatgc
     6421 tgcgccagcg tctcttcgcg tacgccgatc atctgcgctt tggcttcgtt actgtaggtg
     6481 acaaatccgc gttcaaacca ggcggagcta ccggcaatat cggtaatcac tttcgctacc
     6541 caaccaccgg tacaagactc ggcagttgtt acggttgcgc cacgggcttt cagcgcctgc
     6601 ccaacctgtt cacttaactg catcagttca ctgtcagtca tcacaacctc tgtcagtcaa
     6661 aaatttatgc cggacaagat agcactttcg tcaggaagat ggtgttggcg ttgcaatttt
     6721 acgaggagga ttcagaaaaa agctgattag ccagagggaa gctcacgccc ccctcttgta
     6781 aatagctact gtactcgcgc cagcgccacg gcttgtccta actgccagac cgccattgcg
     6841 taatgggtgc tgtggttgta acgggtgatg gtgtagaagt tcggcagacc gtaccagtac
     6901 tggtagccgg tgccaacatc cagacgcagc aggctggctt gttgatggtt gcccagcggc
     6961 tgctgtggcg ttaaacctgc ggcggcaagc tgcgaaatgc tgtacttagt tttgaagcca
     7021 tttggcaagc ctggagcctg accgtttgcc attaccgcga cctgatcgcc tttcacccag
     7081 ccgtgcgctt tgaaatagtt cgccacgcta ccgatcgcat caaccggatc ccacaggttg
     7141 atatgcccgt cgccgctgaa atctaccgca tattgtttgt aagacgacgg cataaactgt
     7201 ccgtagccca tcgccccggc aaaggaacct ttcagattga gcggatcgtc ctgctcgtcg
     7261 cgcgccatca acaggaaggt ttccagctcg ccagagaaat actccgcgcg gcgtgggtag
     7321 ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt tccccatcac gcgcccccag
     7381 cgggtttcaa cgccgataat cccgacgata atttccggcg gtacgccata cacctgccac
     7441 gcgcgattca acgcatcttc atactgattc cagaaaacca caccgttctg cacgttgtcc
     7501 ggcgtaataa attttttgcg ataacgcagc catgcgccgt tcggacctga tggcggtttc
     7561 accgatgtgg ttggtgcctg gttatccatc agccgcagta ccgaatccag acgtttcgcc
     7621 tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac cattttgtcg
     7681 atgaactgct gggcattcgg gttattagcg aaatcgccgc ccatctgcat cacattgtgc
     7741 tgcggctcaa gcaggaagcc gccagacggc gttccggtgg tcgtatcagt ctcagtaggt
     7801 tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt tacataacga
     7861 cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg gtaaagcatc
     7921 acgccccgca caaggaagcg gtagtcactg cccgatacgg actttacata actc
//
LOCUS       DAPGWD010000187         7892 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPGWD010000187 DAPGWD010000000
VERSION     DAPGWD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.188"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            169..897
                     /gene="gadW"
                     /locus_tag="RHX88_003954"
     CDS             169..897
                     /gene="gadW"
                     /locus_tag="RHX88_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDV2232582.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1260..4373)
                     /locus_tag="RHX88_003955"
     CDS             complement(1260..4373)
                     /locus_tag="RHX88_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV2232583.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQAPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4398..5555)
                     /gene="mdtE"
                     /locus_tag="RHX88_003956"
     CDS             complement(4398..5555)
                     /gene="mdtE"
                     /locus_tag="RHX88_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDV2232584.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENTAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5615..5893
                     /locus_tag="RHX88_003957"
     CDS             5615..5893
                     /locus_tag="RHX88_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232585.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5894..6421)
                     /gene="gadE"
                     /locus_tag="RHX88_003958"
     CDS             complement(5894..6421)
                     /gene="gadE"
                     /locus_tag="RHX88_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDV2232586.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSALNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7220..7792)
                     /gene="hdeD"
                     /locus_tag="RHX88_003959"
     CDS             complement(7220..7792)
                     /gene="hdeD"
                     /locus_tag="RHX88_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDV2232587.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2097 a         1890 c         1843 g         2062 t
ORIGIN      
        1 attcgtgttg catttactta ttatcaatta actgttatgc aaaactactt tgtggataaa
       61 ttttggtcct accaaatttg gcagtttttg cgctaagaaa cagtctggca tcatttcatt
      121 agtatactga aattgaaata atcgcagtat gaaatataag ggataatcat gactcatgtc
      181 tgctcggtga tcctcattcg tcgttcattc gatatttatc atgaacagca aaaaatatcg
      241 ctgcataacg agagtatcct gctgctggag aaaaatttgg cagacgattt tgcgttttgt
      301 tcaccggata cgcgacgact ggatatcgat gagctgacag tttgccatta cttacaaaat
      361 attcgtcagc taccacgcaa tttagggtta catagcaaag accgtctgtt aattaaccag
      421 tcacccccca tgccgctggt gacggcgatt tttgatagct tcaatgaatc cggggtaaat
      481 tcaccgatac tgagcaatat gctctacctt tcctgtttat cgatgttttc tcataagaaa
      541 gaactgatcc ccttactttt caatagcatc agcactgttt caggaaaagt tgaacgcctt
      601 attagctttg atatcgccaa acgttggtat ctgcgcgata tcgcggaaag aatgtatacc
      661 agcgagagtc tcatcaaaaa aaagttgcag gatgaaaaca cctgtttcag taaaatatta
      721 ctcgcctcca ggatgtcgat ggccagacga ttactcgagt tacgtcaaat tcctctgcat
      781 actattgcgg aaaaatgtgg ctatagcagt acgtcgtact ttataaacac atttcgacaa
      841 tattatggtg taacgccaca tcagtttgcg caacattcgc caggtacctt ttcctgacat
      901 attttgcatt tgaatattgg tcaggatctc acacctgctt catgtgaaac tcttccctga
      961 tgatttctgc cgggctaccg gctagttctc tttcgcagtt tgccaggcgg tataggtatt
     1021 ttcaggagga aaagactcgg catgtttggg attattaagc tgacaattca tacgattacg
     1081 ggaatgtggg tcagtattgt gctgttcaaa ttgatgacga atggctggag tggtttctac
     1141 tttcagtgct gtgtactaag cctggtattc ctgacggtta gctggttgtt gagtggtgaa
     1201 tggttagcag gaaagagtaa ggctgaacct tcacgttcaa ccttactctc atttacacgt
     1261 tacgcttttt taaagcgggc aaagagatgt tccactacaa cgaaaaagac cggaacaaag
     1321 taaattgcca gcactgttgc ggcaaacatc ccgcccatca cgccggtacc taccgcgttt
     1381 tgcgcgccag aaccggcacc atgactgata accagcggca gcacgccgag aataaaggcc
     1441 agagaggtca tcaggattgg gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc
     1501 ggcgttttcc cttctttctg catcatctca acggcaaatt cgacaatcag gatggcgttc
     1561 ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga agtagacgtc attacttaag
     1621 ccgcgcagat cggtggccag taatgcgcca acgacgccta acggaacaac caacatcacc
     1681 gagaacggaa ttgaccagct ctcataaagt gcggcgaggg cgaggaacac cacgaccagt
     1741 gaaatcgcat acagcgcagg agcctgattt gaggataacg cttcctgata cgacagtccg
     1801 gtccatgagt agccgacgcc tgccggaagt ttagcgacca ggtctgccat aaatttcatg
     1861 gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa tctccattga cgggatgccg
     1921 ttgtagcgtt ccagtcgcgg tgaaccatag gtccattcgg tagacgagta ggcagaaagc
     1981 ggtgccatcg tgccagaggc gttgcgtaca taccattggt tgatgttatc cggcaacata
     2041 cggaacggcg tgcctgcctg gacatacact tttttcaccc gcccctggtt gaggaagtcg
     2101 ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt tgatatcaga cagcgccaca
     2161 cccattgcct cagctttcgc ggcgttgacg ttcactttga acatcggcgt atcttccagg
     2221 ccgttcgggc gtaccccggt gacctgattc ggtgcttgcg ctgccagtga taacagctcg
     2281 tttcgtgcct gggttagttt ttcgtgcccc aggttaccgt tgtctagcag ttccatatca
     2341 aaacctgacg cggtacccag ttcagccacc gcgggtaagt tgaacgggaa gacgacggct
     2401 ttattgatac tgcttagcgc aatcattgcc cgctgaatga tcgcggtaac cgagttttcc
     2461 tcaccgacac gttcagacca cggcttgaga ctgataaacg ccagaccgtt gttttgcccc
     2521 tggccgctga agccaaagcc gccaacggta aacaccgact gaacattatc tttctctttt
     2581 gtaagataat aatccgtcac ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg
     2641 gaaggtaact gcgcggtggt cataaatacc ccctgatcct cttctggtaa gaaagaggtc
     2701 ggcgtgcgca ggaacagcac cgccatcccg gcgcaaatca gcaggtagat caccatgtag
     2761 cgaccggtac aacgcaacag tgagcgggtg ctatcggtat agtgctgagt tgatttttca
     2821 aacagcgtgt tgaagcgtgc gaacagggca ttaggtttgt gaccgccttc cggcgcggct
     2881 ttcagaatgg tggcgcacag ggcaggggtc aggctcattg ccacaaatac tgaaagcagc
     2941 atggaggaga tcagcgtgat ggagaactgg cggtagatct ccccggttgc accgctcata
     3001 aaggccatcg gcataaacac tgcggaaaga acaacggcaa taccgaccag cgcacgttgg
     3061 atctgcccca tcgatttatg cgtcgcttcc ttcggcggta gcttatcttc cgcaatgaca
     3121 cgctcgacgt tctccaccac tacgatggcg tcatccacca gtaaccctat cgccagcacc
     3181 atcccgaaca tagttaacgt gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc
     3241 ccgagaataa ccaccggtac ggcaatcgtc gggatgattg tggcacggaa attctgcaaa
     3301 aacagataca tgaccaggaa gacgaggatg atagcctcaa ccagtgtttt gaaaacttcc
     3361 tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcc
     3421 gggaaatagg ctgataagcg gttcagttcc tctttgactg cccgcgaggt atccagggcg
     3481 tttgctccgg tagccagttt gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc
     3541 acggtggaat aatcttccgc cccaagttcg acgcgggcga catcacgcag cagcacttgc
     3601 gaaccatctt gctgaacttt caacaggatt ttgccaaatt cttccggcgt ttgcagacgc
     3661 gtctgcacaa tgatcgaggc gtttagttgc tggtctgccg cctgtggcat gccacccagt
     3721 tgaccaccag aaatctggtt gttttgcacc ttaatctggg aaataacatc ggaaggtacc
     3781 aggttatatt tattgagttt ttgcgggtcc agccagatcc gcatggcgta ctcggaacca
     3841 aagagctgta cgctaccaac gcccgcggta cggcttagcg ggtctttgat attagacgct
     3901 acatagtccg cgatatcgta ctggttgagg ctgccgttat cagaaataaa cgccgcaacc
     3961 atcaggatat tactgctcga tttatcgacg ctaatcccct gctgctgcac tgcttcaggt
     4021 aatgaaggca tagcgagttg cagtttattt tgcacctgaa cctgtgcgat atcaggagat
     4081 gtcccagtct cgaaggtcag agtgatagag gcattacccg ccgcatcact ggttgaagac
     4141 atgtacatca ggccatcaag cccattcata ttttgctcaa tcacctgagt gaccgagtct
     4201 tctaccgttt gcgcatcggc acccggatag gtagcgctga cggtaatggt cggtggcgca
     4261 atctgcggat actgcgcaac cggtaagttc atgatcgcca gaccacctgc aagcatcata
     4321 ataatggcaa gtacccaggc aaaaaccggg cgatcaataa aatagttagc catgaaagtc
     4381 cccttaagcc tgcaacgtta ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa
     4441 attgctcgtg ctttgatacc cggacgaatg cgttgcaaac cggaaacgat cacccgatcg
     4501 ccagcctgca agccagaggt gacaacccac tggtcgccga tggctttgct ggcttcaatt
     4561 tcgcgtagct gcacgacatc gtctttatcc agaatgagcg ccgttgcttt accctgggcg
     4621 ttgtgggtga cgccttcctg cggcaccagt aatacattct ggcggctacc ttcatccact
     4681 aatgccgtga catacatgcc aggcagcaag tcaccatttg ggttggggaa aatcgcccgt
     4741 aacgtcacgg agcccgtggt ttcatcaacc gtcgggtcgg agaatttcag cgtgccggtc
     4801 tggctgtaac gtttaccatt ttccagattg agctgtactg gcgtactgcc ctgaacctgt
     4861 ttgatttgcc cactggcgac ctcttctttc atgcgtaaga aatcttgcac cgactgcgtg
     4921 agatcgacat aaatcgggtc cagacgttgt acggtaacca gcgaatctgc ctgattagcg
     4981 gtaacgagtg cgccgacggt caccgacgat ttcccgctga cgcccgtaat cggcgaggtg
     5041 acattcgcgt attgcagatt gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca
     5101 ttggcttctg cttcattcaa ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag
     5161 ttggtcttca gcaacgatgc ctggcggtta aaggtgatac gggcattgct ggcggtagag
     5221 agcgctttcg ccagcgagcc tttggcggag tttagctcgg cctgtaaagg tgcaggatca
     5281 atctgataca gcgaatcgcc ctggttcact ttatcgcctt cgataaagtt gcgtttaatg
     5341 ataataccgc ccacctgggg acgtatctcg gcaacttcat aaggaacggt tctaccgggc
     5401 aattcgctca acacattgac cgacccaggg gagagtgtga cgacaccgac ctcaggcgtc
     5461 atggcggcgg tgttttccgc cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat
     5521 aacaacggta ttaacagctt tcttcttctg ttcattttat tccctgaaaa ttcttgagaa
     5581 ataacggttc tttaaattgc gcgcgaggag agatatgagg gatttacaga ccagcggcat
     5641 agtggggttg tcagccagta aggtgggata caggcatagt gatcgacatg gtgaggtcaa
     5701 cgacaaaaaa aatgttgccg ttctgccaac agttcctgcc agcattcggg caacgaaggg
     5761 ttctactggt ggatacacat accaggggaa taaagtcgtt ttagcctctc ctttagtaat
     5821 caccgggggt aatgaaatca gcatctgcat ccctcgtcat gccagccatc aatttcagtt
     5881 gcttatgtcc tgactaaaaa taagatgtga tacccagggt gacgatgtcg ctcatacgtc
     5941 ttagtttcag cttgtacata atgttgaatt tctgaatctt taatgtttgt tgcgaaatat
     6001 taagcgccga cgtgatattg ctttcatttt tatttttcag aatcagttgc ataattttga
     6061 actgattatg tgaaaaacat aattgtgaat tcttatgggg caagtgttta ccataagtaa
     6121 tctcaagaag atttttgata ctttctttgc ggctaacaaa ataaataggc gctttagctt
     6181 ttagtttact gatgtgataa ggtgccaaaa caataattct ctcggcatct aatttctcca
     6241 gaaatttaat cgcttcttca tcaaggatat gattgtgata ggtatcaatg ataaccttga
     6301 agggtttatt gcctactttt ttgatctctg acagggaatt gatttttatc atttcgtgat
     6361 tatctttcaa ctgccaaaag ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca
     6421 taacttgctc cttagccgtt atcgtttcga acatgtcatc cttttcatta tttacatcct
     6481 tgtccgaatc gttgttcaat atagtaaacg cctataagaa ctttgccctt cgtgaactaa
     6541 tacgtctatt aacactaatc aatacttcag atttatccat cacgcactcc atatattgat
     6601 cctaagtaag attcctggtt gttatcagct tgtaaatagc aaacaaaaag ttaacgtttg
     6661 cggattactg cccaagaata agttatcaat aaccagtttg tgatctctga agaatattac
     6721 taaagttaaa atgtaatccg atttaaatat cgagtctcct tgtttcgact taagctggca
     6781 attggattgc cagctttctt ttaacgcatt gaaattacct gagtaaataa cacattcatg
     6841 tcttcaggtt tttatatagc gtctggagac acggatattt atgcaatgta atcaacgcca
     6901 gttgcaaaaa aatgaacccg gattacttat ttaaccgccc atagcatcaa cgcttacgga
     6961 actctttgtt cactttccca cagcactttt aaaacattaa acctgactac ggaaaatatc
     7021 agccatgata atgtttgaaa tggctaattt gccatggagt gaaaaaaatt agatgaaatt
     7081 cagtaggttg aaataatcac tagcaggtaa ttatttcaat gatagtgcgc aattgatcta
     7141 caacactgcg tagcggagag agtattaatc ggatcatagt cacatcaagt gactatgatc
     7201 cgggtgacaa ccgggataat tattgctgct taacgaacaa actggcgaag ctgaacaggc
     7261 tggcggcgct aaatatcagt tcaattccca ccagtgtgga aaccagcgtt acagacacca
     7321 tcggcgttgc accaaggaat atccaggcaa tgacgatatc cagcacacca ataacgagct
     7381 gtagccagct gcctttcatt gaacgctgac gataccaact catcaggcga ataacccctg
     7441 caacacagaa caaaccggca ataaatgccg caatggcaaa aatgcccagc tccggtgcgc
     7501 ggatgaagaa atagccgatc aataaatagg cgactgcgac gaggaaaccg gataataccg
     7561 gccagaaatt atgactgcgg ttgctgaata acccgacaat aagcgcaata cccgagcaga
     7621 ttaataatgc acccactact gtgcttaaaa tatcgccaga gacgaacggg aaactgatac
     7681 acagcaaccc gacgataaac agcagcacgg caataaactg gattgctctg cgatgctttt
     7741 tcagcatctc cagatcaaac ttcaaaattg ttgccttatc tatatataac atagaaccac
     7801 cctataaaat taagaagaaa atcccctgct atcaatctat gccaaaaacg cgtctaagaa
     7861 tgcagtcgat ttaataaaaa tttcctaatt gc
//
LOCUS       DAPGWD010000188         7866 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPGWD010000188 DAPGWD010000000
VERSION     DAPGWD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.189"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            37..555
                     /locus_tag="RHX88_003960"
     CDS             37..555
                     /locus_tag="RHX88_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDV2232588.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
     gene            complement(600..2645)
                     /gene="dcp"
                     /locus_tag="RHX88_003961"
     CDS             complement(600..2645)
                     /gene="dcp"
                     /locus_tag="RHX88_003961"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDV2232589.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2782..3528
                     /gene="ydfG"
                     /locus_tag="RHX88_003962"
     CDS             2782..3528
                     /gene="ydfG"
                     /locus_tag="RHX88_003962"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDV2232590.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3617..4303
                     /gene="ydfH"
                     /locus_tag="RHX88_003963"
     CDS             3617..4303
                     /gene="ydfH"
                     /locus_tag="RHX88_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDV2232591.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4481..4684
                     /gene="ydfZ"
                     /locus_tag="RHX88_003964"
     CDS             4481..4684
                     /gene="ydfZ"
                     /locus_tag="RHX88_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDV2232592.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4720..6180)
                     /locus_tag="RHX88_003965"
     CDS             complement(4720..6180)
                     /locus_tag="RHX88_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV2232593.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDEKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(6269..7552)
                     /gene="ydfJ"
                     /locus_tag="RHX88_003966"
                     /pseudo
     CDS             complement(6269..7552)
                     /gene="ydfJ"
                     /locus_tag="RHX88_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT         2071 a         2012 c         1814 g         1969 t
ORIGIN      
        1 aatcaccttt acatataata gcgtggacaa aaaacagtga acattaataa agataaaatt
       61 gtacaactcg cagataccga cactattgaa aacctgacat ccgcgttgag tcaaagactt
      121 atcgcggatc aattacgctt aactaccgcc gaatcatgca ccggcggtaa gttggctagc
      181 gccctgtgtg cagctgaaga tacacccaaa ttttacggtg caggctttgt tactttcacc
      241 gatcaggcaa agatgaaaat cctcagcgta agccagcaat ctcttgaacg atattctgcg
      301 gtgagtgaga aagtggcagc agaaatggca acaggtgcca tagagcgtgc ggatgctgat
      361 gtcagtattg ccattaccgg ctacggcgga ccggagggcg gtgaagatgg tacgccagcg
      421 ggtaccgtct ggttcgcgtg gcatattaaa ggccagacct acactgcggt tatgcatttt
      481 gctggcgact gcgaaacggt attagcttta gcggtgaggt ttgccctcgt ccagctgctg
      541 caattactgc tataaccagg ctggcctggc gatatctcca ggccagccat tggtggtgtt
      601 tatatgttca agccacgatg ttgcagcatc ggcataatct gaggagcctt accgcgccat
      661 tgtcgataca ggcgttccag atcttcgctg ttacctctgg aaaggatcgc ctcgcgaaaa
      721 cgctgcccat tttcacgcgt taatccgccc tgctcaacaa accactggta accatcatcg
      781 gccaacattt gcgtccacag ataagcgtaa taacctgcag catatccgcc accaaaaata
      841 tgggcgaaat aactgctgcg atagcgtggc ggtatagcag gaagatccat attttccgcc
      901 accagcgccc gcaattcaaa atcatcgaca tcctgcattg cttcgttttc ttccaggcaa
      961 tgccagcgca tatcgagaag tgcggcgcta agcagttcgc tcatctcata ccctttgttg
     1021 aacaggctgg cattacgcat tttctgttgc agttcgtcag gcattgctgc cccgctctga
     1081 taatgccggg cgtagcgagc gaatacctgc ggatgcgttg cccagtgttc gttgatttgc
     1141 gacggaaatt cgacaaaatc acgcggcgtg ttggtgccgg aaagcgtggc ataacgctgg
     1201 cgggcaaaaa ggccgtgcaa cgtatgacca aattcatgga ataaggttat gacatcatcc
     1261 cagagtaaca acgcaggctc accggcagcg ggtttctgat aattgcaaac gttataaatt
     1321 accggatgcg tttcattaag cgttgattgc tcaacaaaat tgcccatcca tgcaccgccg
     1381 ctttttgaat cacgggcgaa gaaatcaccg taaaataacg ccagccccac gccattatga
     1441 tcaaaaattt cccacacccg aacgtcagga tggtagacag gaatatcaaa acgttcgaca
     1501 aacttaatac cgaagagctg attcgcggtc cagaatacac cttcattcaa caccgtgttt
     1561 aattcaaaat atggcttgag ctgcgcctca tcaagatcaa atttctcccg ccgtacctgt
     1621 tcggcataaa atgcccagtc ccacggctgc gcgctaaacc cgccctgctg cttatcaata
     1681 accgcctgta tggaggctaa ttcatcgctc gcacgttgac gcgccgctgg aacaatttcc
     1741 cgcataaagt tgagcgctgc ttcaggtgtt tttgccatct gatcggcgat tttccatgcg
     1801 gcataatgag gaaaaccaag taatgtcgcc tgttgtgcac ggatctccac cagacgttga
     1861 atgatagcgc gggtatcatt gccatcattt ttttccgctc gcgtccagcc cgcaataaac
     1921 agtttttcac gcgtcgcacg atcgcgcatt tcggcaagcg ccggttgttg ggtggtattc
     1981 agcagcggaa tcagccattt gttatccaga cctttctcgc gagccgcctc tgccgccagc
     2041 gcaatctctt gctcactcat tcctgccagc tgcgcgatat cgttcacaac cagaccgccg
     2101 gatttatttg ctgccagtaa ccgctggtta aactgactgg tcagtgtcgc agcttctgta
     2161 ttcagtactt ttaattttgc tttatcagct tgcgcaagtt tggctccggc aaggacaaaa
     2221 cgttgatgaa tcacctccac caggcggatg gattcactat caagccccag ggattcacgg
     2281 cgctgccaga cagcatctac ccgcgcgaat aattcaccgt tcagatagat atcattagcc
     2341 agttccgcca gttcagcgga aaactgttca tcaagacgct gtaattcatc attggtatgc
     2401 gccgcagtca tcgcaaaaaa gacgctggta acgcgggtaa gtaattctcc gctttgttcc
     2461 agtgccagaa tagtattgtt gaaatcaggc gtttgcgggt taagcgcgat ggcagcaatt
     2521 tctgcccgct tttgctgcat tccctcatcg aatgccgggc gatagtgatg attggcaatt
     2581 tgatcaaaat gaggagccag atacggcagt gtgctttgca caaggaaagg attcattgtt
     2641 gtcattttct tctcctgaac gcgaggtgtt ccatagcgta ggcttactga taacggagtg
     2701 caatcttgca atccagtatt acccgctctt aagcatcccg tgctatgtta ttgacacaca
     2761 aaagcgttga ggaacagtga gatgatcgtt ttagtaactg gagcaacggc aggttttggt
     2821 gaatgcatta ctcgtcgttt tattcaacaa gggcataaag ttatcgccac tggccgtcgt
     2881 caggaacggt tgcaggagtt aaaagacgaa ctgggagata atctgtatat cgcccaactg
     2941 gacgttcgca accgcgccgc tattgaagag atgctggcat cgctccctgc cgagtggagc
     3001 aatattgata tcctggtaaa taatgccggc ctggcgttgg gcatggagcc tgcgcataaa
     3061 gccagcgttg aagactggga aacgatgatt gataccaaca ataaaggcct ggtatatatg
     3121 acgcgcgccg tcttaccggg tatggttgaa cgtaatcatg gtcatattat taacattggc
     3181 tcaacggcag gtagctggcc ttatgccggt ggtaacgttt acggtgcgac gaaagcgttt
     3241 gttcgtcagt ttagcctgaa tctgcgtacg gacctgcatg gtacggcggt gcgcgtcacc
     3301 gacatcgaac cgggtctggt gggtggcacc gagttttcca atgtccgctt taaaggcgat
     3361 gacggtaaag cggaaaaaac ctatcaaaat accgttgcat tgacgccaga agatgtcagc
     3421 gaagccgtct ggtgggtgtc aacgctgcct gctcacgtca atatcaatac cctggaaatg
     3481 atgccggtta cccaaagcta tgccggactg aatgtccacc gtcagtaatt tttatacccg
     3541 gcgtaactgc cgggttattg cttgtcacaa aaaagtggta gactcatgca gttaactcac
     3601 tcacaagcaa gaacgaatga ccgtcgaaac gcaacttaat cccacacagc ctgtcaatca
     3661 gcagatttat cgtattcttc gtcgcgacat tgtccattgc ctgattgcac caggcacacc
     3721 gttgtcggaa aaagaagttt ctgttcgttt caatgtgtca cgccagccgg ttcgtgaagc
     3781 ctttattaaa ctggcggaaa acggcctgat tcaaattcgt ccgcagcgtg gcagctacgt
     3841 caacaaaatt tccatggccc aggtgcgaaa cggcagtttt atccgtcagg ccattgagtg
     3901 cgcggtggcg cgtcgggcgg cgagcatgat taccgaaagc cagtgctacc aactggaaca
     3961 aaatcttcac cagcaacgca ttgccattga gcgcaagcaa ctggatgatt tttttgaact
     4021 tgatgacaac ttccatcaac tcctgacgca gattgccgac tgtcaactgg cgtgggatac
     4081 cattgagaac ctgaaagcga ccgttgatcg cgtgcgctat atgagtttcg accatgtttc
     4141 tccaccagaa atgctgttac gccagcatct tgatattttc tctgccctgc aaaaacgtga
     4201 tggcgatgcg gtagaacgtg caatgacgca acatttgcag gaaatcagcg aatccgtgcg
     4261 ccagatccgc caggaaaaca gcgactggtt tagcgaagag taattcattt actctcatcc
     4321 catccgggat gagaggcttt tccccccgcc ttacggctca tgcatgcatc aaaaaagatg
     4381 tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat tgcgcccgtt
     4441 acgtgggctt cgactgtaaa tcagaaagga gaaaacacct atgacgacct acgatcgtaa
     4501 ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc acactggcaa
     4561 gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg gaaaaaccgt
     4621 agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc tcggcatgaa
     4681 ctaagtgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg aatatttcgc
     4741 cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca cttttgcagt
     4801 aaacaagcta ttgtctggca aatcatcacc aaagattgcc ttaatcgcca gcaatgactg
     4861 gacgcgcgct ttcccttcgg cactactttg tacagccttc tgaataacag gtaacagtgg
     4921 gtcactgatt tctatcggat ttccctgttc atcaacacca ccgacataac gcatccaacc
     4981 cgcgacgccc agcgccagca gatcgaactt gctgtcatgc gccagatgcc agcgaacaga
     5041 atccaacatc cgctgtggca atttctggct accatccatc gcaatctgcc aggttcgatg
     5101 acgtaacgcc gggttgctat agcgtgcaat taatcggtta gcgtaatctt gcaaatcaac
     5161 gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg ccgcataacg
     5221 ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat aacccaaata
     5281 cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat aaggcagcac
     5341 atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa caaagttatc
     5401 ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc ccacaccggt
     5461 aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac ggtccaccat
     5521 tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agtttttcat caacggcttt
     5581 cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt tgtcacatga
     5641 catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct caacaatcac
     5701 ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca tcgggtgatc
     5761 gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga ttgtcagaga
     5821 gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc catccatctg
     5881 tacgtgcaag gcttttttaa cgacgccaac gacgcgagcc gtccacgcat cggccgacat
     5941 ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct gttcgccgcc
     6001 gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa gaatatcggc
     6061 atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaataa ttcttggggc
     6121 caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat tatttcccat
     6181 aacaattcct taaatttaaa tatggcaagc tatatgtttt gttatatgaa taaaaatccc
     6241 ctctccggta agagaaggga ttgagggttt acagacttct ggaaggttgc gcagctctta
     6301 caacacgcgg ttgatcttcc gcagcgtctt ccagcgcact taaatcacgg tctttcacct
     6361 ctggcatttt cagcgcagag attaaaccaa ttactgaata tgccatgatc ataatggcga
     6421 tcggatacca ggattccgtc atggtgcaga aaatacccgc caggatagga ccaaaaccgg
     6481 aagcgataag accaccaatt tctttagaaa tagccatccg ggtaaagcgg tttttacagc
     6541 cgaacatttc tgccatggta atgttttcca gagcaaataa tcccagcacc gcacagttat
     6601 gaatcacaat cagtgcaacc ataatggtgc tcggggcata gcttttatct acaatgatag
     6661 aaagcattgg ccatgccagc acaatcgcgg aggtattcat aataatatac gggatccggc
     6721 gaccaatttt atcggataac caaccaagga acggaatggt cataaagccg agaatcgaac
     6781 taatcatcaa tgcatctgtt ggaattgctt tgttaaacaa taacgtctgc actaaatagc
     6841 ctgcaaggaa agtctgaatt aacccggagt tacccgcctg accaaaacgc agccctgttg
     6901 ccagccagaa ggatttgctc tggaacatgc taccagcagg tgcaggtttt gctgtgggtt
     6961 ggttgctgtc attaaccttc tcaaagaccg ggctttcttt cagattcata cgtaaccaga
     7021 tagcaaagac catcacgaca acgctcgcca ggaacggtat acgccatccc cacgccagca
     7081 gttcctcttt actgagaatg aagaacataa aggcccagat tgccgttgcg ctcaaggttc
     7141 cgcagttagt tcccatagct acaaatgagg agataattcc gcgcttacct tttggtgcat
     7201 attccgccag catcgtaccg gcaccggaaa tttccgcacc tgcacccaac ccctgaataa
     7261 tacgcagcgt caccagcaag atgggggcaa aaacaccaat ctgtgcatag gtcggtaaca
     7321 caccaattaa ggtggtacag atccccatca tggtgatggt aataaagagc acttttttac
     7381 gccctattct gtcgcccatt ttgccgaaaa taaatgctcc gacaatacgc gccacataac
     7441 ctgcaccgta ggttcccatt gccaaaatta acgccattgc cgttgatgat tcaggaaaaa
     7501 atatttcatg aaacactaac gctgcgccga gcgaatataa ctggaaatcc ataattcaca
     7561 ggtgtttttt cccatcctgt ggtttccttg gcgttttcta ggttttttca gatagttgca
     7621 ttttttaaaa agcatcctaa gttcgatctc agtgtctatc tggggcctat ttctgtccca
     7681 tatatgcccc aaaaaaactc cccaacagat aagtagtttt ttcatggatt tatgcgtaaa
     7741 atcaagaacg gctggaaatc attcaatact cacactatcg aaaaatttac cagccaaccg
     7801 cagcacgttc ttgcataagg cgtgtctgcg ttttttcaac tattcagata catcactccc
     7861 atcaca
//
LOCUS       DAPGWD010000189         7853 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPGWD010000189 DAPGWD010000000
VERSION     DAPGWD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.190"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(539..1237)
                     /locus_tag="RHX88_003967"
     CDS             complement(539..1237)
                     /locus_tag="RHX88_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232594.1"
                     /translation="MDKTRDFILGGLSRFGLADLPGRPYDSAFELIASPPIRKRLIVM
                     GFNGSSADSHMTNSQSIIQDHSKPLVSNVHLGTQGEWGITRLAKRLQQLPISLGYRWE
                     DVVFTNALMMCSTNASELKQEASRHKLTVEQLIKYSTRFFENVTIPLCKPELIIAYSN
                     SLQSLSAANILLKNFGDPSTLIYTQQKGYYTTFSFSAVLNDVKIPVICVRHMSRFKPS
                     EELIETTLETMRGL"
     gene            complement(1616..3949)
                     /locus_tag="RHX88_003968"
     CDS             complement(1616..3949)
                     /locus_tag="RHX88_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDV2232595.1"
                     /translation="MKMNVTETVKQACGHWPRILPALGVKVIKNRHQACPVCGSSDRF
                     RFDDKEGRGTWFCNQCGAGDGLKLVEKVFGVTPSEAAGKVNAVTGNLPPVAPEVIAAA
                     EAETEADRKAAAALAARLMEKTRPATGNAYLTRKGFPALECLTLTVMHKTGGVTFRTG
                     DVVVPLYDDTGALVNLQLINADGLKRTLKGGQVKGACHVIEGKKQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQNKAAAAAD
                     AYEGIVALPPVFGDWNDAFVQNGGEATRKAIYDAIRPPVQSPFDTMSEAEFTAMSASD
                     KALRVHEHYGEALAVDANGQLLSRYEAGIWKVVQPSNFERDVAGLFQRLRAPFSSGRI
                     TSVVETLKLIIPQQAAPARRLIGFRNGVLDTRSGLFSPHSKSHWLRTLCDVDFTPPVE
                     GETLETHAPNFWRWLDRAAGKNPQKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATLLAGEDNATSADIDTLEDPRKRASLIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNPYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPEERD
                     PQLRDKIARELAVIVRQLMQKFSNPMTARALLQSQQNSDEALSIKRDADPTFDFCGYL
                     EMLPQTNGMFMGNASIVPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPMMLKEY
                     GLNYEKRHTKQGIQTNLSLKEESYGDWLPKCDEPAAT"
     gene            complement(3964..4284)
                     /locus_tag="RHX88_003969"
     CDS             complement(3964..4284)
                     /locus_tag="RHX88_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HDV2232596.1"
                     /translation="MKKPLPPVLRAALYRRAVACAWLTLCERQHRYPHLTLDALESAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMEAGPLKAAPSLSFLGLAVMDELCARHITA
                     PVLH"
     gene            complement(4281..4508)
                     /locus_tag="RHX88_003970"
     CDS             complement(4281..4508)
                     /locus_tag="RHX88_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232597.1"
                     /translation="MRHTTITARDLECLEHMRNVGQLVNELMQVQDCTSVRRDPAQQL
                     QLTSVIYLMTAQLDGVVERCNQQWLTEEGNV"
     gene            complement(4505..5053)
                     /locus_tag="RHX88_003971"
     CDS             complement(4505..5053)
                     /locus_tag="RHX88_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDV2232598.1"
                     /translation="MMMTVQQTAPFSGLLLFAVSRYSFSAVAKSAAGRENPSYSVATP
                     DAPCVFFCVYAYVHLLFTHRFLSRCCICVMVAQAGQPSGWPVPIEAGFSPPSGLPPER
                     ENSGGSSNRYSMEAAIMATTLTPSHPQFVFVFAAVRRTDRKPRICMLRTVAGDEHAAR
                     LSLARDYVLSFAGRLPVAEVRA"
     gene            complement(5050..5316)
                     /locus_tag="RHX88_003972"
     CDS             complement(5050..5316)
                     /locus_tag="RHX88_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV2232599.1"
                     /translation="MHTAFSSPSSAPAAPLMPVSDTVHERFIRLPEVMHLCGLSRSTI
                     YDLISREAFPKQISLGGNNVAWAQSEITAWMADRIAERNRDYDA"
     gene            5854..6597
                     /locus_tag="RHX88_003973"
     CDS             5854..6597
                     /locus_tag="RHX88_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDV2232600.1"
                     /translation="MKPETVITALQNVAAQQSAESSQRITYKLSAFTAARDTHAASMQ
                     ELKEIDTSIERCKQERQTALSESAEAEQDWRSRFRTLRGNLTPEMKAEHSKRIASREL
                     ADEFTGLIAELETDRTRAMLNACSTGNKYLSAHDDAFTTYAGAEWAQAVNTLPAALIR
                     AFLLRIRALEMKGDSAPQSVAIGELRDALSRQGSLYQFDMKQEPVLSVTGMHRPQITG
                     VDMELLRSPAKRMMLARKLSENDGTKVEA"
     gene            6600..6818
                     /locus_tag="RHX88_003974"
     CDS             6600..6818
                     /locus_tag="RHX88_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013095389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDV2232601.1"
                     /translation="MFHCPFCKTSAHARTSRYLSENVKQRYHQCVNIECSATFRTLES
                     VDGIICPPPTEPVIPASEPAATVNRAGA"
     gene            6847..7410
                     /locus_tag="RHX88_003975"
     CDS             6847..7410
                     /locus_tag="RHX88_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HDV2232602.1"
                     /translation="MTTLMLQKAFEACQANKSAWLQRRDELKQAEQAYREQLAGNGHS
                     GRSLKILREIIDVKKWEINQAAGRYIRSHEEVQRISIRNRLNDFMQVHGAELAAALAP
                     ELMNYSGQNSAVQRCAMQHSVDYLREALQVWLTAGEKINYSAQDNDILTAIGFRPDAA
                     SRDDSREKFTPAQNLNYTRRRAELAAQ"
BASE COUNT         1854 a         2026 c         2151 g         1822 t
ORIGIN      
        1 caagttgatt tgatatatta aatgaacaaa ttaatcttga tggcagtctg attattggtg
       61 aagtttatag tcagtttttt tcgccagcgg ttaaagaaat agaatagtcg tataaaaaag
      121 ggtgttctgg ggcgagtaaa acaacatctt caccttaatg gcgatacagt tggctgagag
      181 tcagcagggg ggcaaatcac ctgcaaataa acacggcaca tgctggcgcg catatcgcgg
      241 atgcgttcga ggacagaaga accgacaggc ggattcttca gccgctcatc gtccatcaca
      301 aaaccttaga ccaggtattc ctgaagccga ttgaatattg agcagtagaa aatatctgga
      361 ttgacagtat tacaaggggt tggagaacgt caggagaggc attttggcgg agatcacagg
      421 agttcttatt attacttaac ttgctgtttt tgttgtattt tattttaagg tggtgttttt
      481 gttatacaca tcgctataca cattgctcca agcgtatggt tcaatctgac ggataaagct
      541 aaagccccct catcgtctct agagttgttt caattaactc ttctgaaggt ttaaatctag
      601 acatatgacg gacacatatt acagggattt tcacatcatt tagtacggct gagaaagaga
      661 aagttgtgta ataacccttc tgctgcgtat agatgagagt gcttggatca ccgaagtttt
      721 ttagcaaaat atttgctgct gaaagggatt gtagactgtt gctataagcg ataatcagtt
      781 caggcttaca cagagggatt gtgacattct caaaaaatct tgttgagtat tttatgagtt
      841 gttcgacagt tagtttgtgt cggctcgcct cttgtttgag ttctgaggca ttggttgagc
      901 acatcattag agcattcgta aatactacgt cttcccatct gtaaccgagg ctgataggta
      961 actgttgtag tcgcttagct aggcgagtaa ttccccattc cccttgcgtc cctaaatgga
     1021 cattcgagac aagaggcttt gaatgatcct gaataataga ctggctgttg gtcatatgag
     1081 aatcagctga cgaaccattg aagcccatca caatgaggcg tttgcgtata ggtggggagg
     1141 ctattaattc aaaagcagaa tcgtaaggtc tgccgggcaa atcagccaag ccaaagcgag
     1201 aaagccctcc aagaataaag tctctagtct tatccattat tcaatttctc ttgtgaagcc
     1261 tcataccgac atcatacgca aaaaaacttt ttttgcattt aactgttcac actgttcacc
     1321 tcggttattt ctcattttat atcatgcagt taagtggtga tgagttggtg aagggtgaac
     1381 agtcgactct tcacctttgt tcgttttgct cgttctgggg gcgcccggtc aggcgataca
     1441 cggggggata aaaagttttt tccggtttta ctgttcacac tattcaccat tgctttttta
     1501 tcaataattt catgctaata cagggtgaat atacggcgaa gggtgaacgg tggattgttc
     1561 accttatgac aattgccgga aagaaaaaga ccggctgtag ccggtctgga gtgggttatg
     1621 tcgctgcggg ttcgtcacat ttcggcagcc agtcgccgta gctttcctct ttcagcgaca
     1681 ggttggtctg tatcccctgc tttgtgtgcc gtttttcata attcaggccg tactctttca
     1741 gcatcatggg cagccccagc ccgaacattt tcaggctgag cacgtttctg tacccgttag
     1801 cctccatata ggccagatac gcgtgataaa gatatttacg gtaattacgc gggacaatgc
     1861 tggcattacc cataaacatt ccgttggtct gcggcagcat ttccagatag ccacaaaaat
     1921 caaacgtcgg gtcagcatca cgcttgatgc tgagcgcctc gtcggagttc tgctgcgact
     1981 ggagcagtgc gcgggcggtc atcgggttgc tgaatttctg cataagctgg cgcacgataa
     2041 cggccagctc gcgcgcaatt ttatccctga gctgcgggtc gcgttcttcc ggcgcaatct
     2101 gctccgggaa atgaataatc acccggcggc gcgacacacc gccgctgcgg tcggtaaagc
     2161 gcatcgggtt attgttgacg gccagaatga ccgccggaat atgcgttgaa tacgggttct
     2221 ggtatttcgg gtcaaccgag accgcatcgc cgccggtgat ggcctttaat cctgcgccgt
     2281 caccgctcca tttttcctgg tcaggcagac ggataagcga gaagccaatc agggaggctc
     2341 gcttgcgcgg gtcttccagc gtgtcgatgt cggccgacgt ggcgttatct tccccggcga
     2401 gcagggtcgc gatttcggcc agaatacttt tgccgctgcc acccggaccg gtgacttcga
     2461 gaaagagctg ccagtcgtag cggttcgcaa gcaccatgaa caacgcggcc agtatcacgt
     2521 cgcgcttttg tggattttta ccggcagcac ggtcgagcca gcgccagaaa ttcggggcat
     2581 gggtctccag cgtttccccc tcaacaggcg gggtaaaatc cacgtcgcac agcgtgcgca
     2641 gccagtgcga tttgctgtgc gggctgaaca ggccgctccg ggtatcgagt accccgttgc
     2701 gaaaaccaat cagacggcgt gccggtgcgg cctgctgagg aataatcagc ttcagcgtct
     2761 ccaccactga ggtgattctc cccgacgaga acggggcgcg caggcgctgg aaaagcccgg
     2821 ctacgtcgcg ttcgaaattg gacggctgaa cgactttcca tatccctgct tcataacggg
     2881 acaggagctg gccgttcgca tccactgcca gcgcttcgcc gtaatgctca tgcacccgca
     2941 gagccttgtc gctggcgctc atggcggtaa attccgcctc gctcatggtg tcgaacggac
     3001 tttgtaccgg tggccggatg gcgtcataaa tggctttccg cgtggcctcc ccgccattct
     3061 gcacaaacgc atcattccag tcaccgaaca ccggcggcag ggcgacaatg ccctcatagg
     3121 cgtctgcggc cgctgcggct ttgttctggc cgtcgccgct caggtcgcgg tcggcggcga
     3181 gcacaatctg acaggccggg tgtttctgac gggcaaggct cgccagagaa aggaggttca
     3241 cggacgacag cgccaccatg acggtttcgc cggtcaggtg atgcacggtg agtgcggtcg
     3301 cataaccctc cgcaatccac aggcgttttc cggcctgttt tttcccttcg atgacatgac
     3361 atgccccttt gacctgaccg cctttcaggg tgcgtttgag accgtcagca ttgataagct
     3421 gaaggttaac cagtgcgccg gtatcgtcat acagcgggac aaccacatca ccggtgcgga
     3481 acgtcacgcc gccggtttta tgcatgacgg tgagcgtcag acattccaga gcggggaaac
     3541 ccttgcgggt gaggtaggca ttgccggtgg ccggtcgggt tttctccatg agcctggcgg
     3601 ccagcgcggc cgccgctttg cggtcggcct ccgtttcagc ctctgcggcc gcaatcactt
     3661 ccggggcaac cggcggcagg ttgccggtca cggcgttcac cttcccggca gcttctgacg
     3721 gggtcacgcc aaacactttc tctaccagct taagcccgtc acctgcgcca cactggttgc
     3781 agaaccacgt cccgcgcccc tctttatcgt caaagcggaa acggtcagag ctgccgcaca
     3841 ccggacaggc ctgatgccgg tttttaatga ccttaacacc cagcgccggg agaatgcgcg
     3901 gccagtggcc gcacgcctgt tttaccgtct ctgttacgtt cattttcatc gttgttttct
     3961 ccctcagtgc agtaccggtg cggtgatatg acgggcgcaa agctcatcca tcacggccag
     4021 ccctaggaag gacagcgagg gggcagcttt gagtggtccg gcttccatta aatcttccag
     4081 cagtgcacag gcaatctggc ggcctttttc ctcgccgtgc tggcgcaggt agaagccctc
     4141 cagctcggcg gcaatagcgc tttccagcgc gtcaagggtg aggtgcggat agcggtgctg
     4201 gcgttcgcac agggtcagcc atgcacaggc cacggcgcgg cgatacagcg cggcgcgtaa
     4261 tacgggaggt aatggctttt tcatacgtta ccctcttcgg tcagccactg ctgattgcag
     4321 cgttcgacca cgccgtcgag ctgggcggtc atgaggtaaa tcacggaggt aagctgtaac
     4381 tgctgcgcag ggtcacgacg aacggacgta cagtcctgca cctgcatcag ctcattgacg
     4441 agctggccga cattgcgcat gtgctcaagg cattcgaggt cgcgggcggt aatggtggtg
     4501 tgtctcatgc gcgcacctcc gcaaccggca gacgaccggc aaacgagagg acgtaatcgc
     4561 gagcgaggga aagacgtgcg gcgtgctcat caccggcaac ggtgcggagc atacagatac
     4621 ggggtttacg gtctgtgcga cgaacagcgg caaacacaaa gacaaactgc gggtgtgacg
     4681 gggtgagggt cgtagccatg atggcagcct ccattgagta gcggttactg ctaccaccgg
     4741 agttctcacg ctcgggtggt agcccagacg ggggtgagaa accggcctca atgggtaccg
     4801 gccagcccga aggctgcccc gcctgagcca ccattacgca gatacagcaa cggcttaaga
     4861 accgatgcgt aaacaacagg tgcacatagg catagacaca aaaaaagacg catggcgcgt
     4921 ccggtgtcgc cactgagtaa ctcgggttct cacgcccggc tgccgatttt gcgacagcgg
     4981 aaaaactata cctggaaacg gcgaaaagaa gcaagccaga aaaaggggct gtttgctgaa
     5041 cggtcatcat catgcgtcat agtcccggtt acgttcggca atgcgatccg ccatccatgc
     5101 agtgatttca gactgagccc acgccacatt atttccgccg agggatattt gcttcggaaa
     5161 agcctcccgg ctgatgaggt cgtaaatagt cgagcgggac aggccgcaca gatgcatcac
     5221 ttcggggaga cggataaagc gctcgtgaac ggtatcagaa accggcatca gcggcgcggc
     5281 aggggcagaa gacggggaag aaaaagcggt gtgcatcggg ctacctcata atgtccatac
     5341 agtgccggtc atgtccgtcc ggcttcgggt agctccttat tatgtctata tttttcctca
     5401 ggtcatgtga gatttttggg gaaacaaaca ttgacttttc gctatggcaa acaaaggaaa
     5461 acgctggcaa acatatgcaa atcactgcat tacaatgcag caatttctat tgcttttagt
     5521 tatacgtttt tcatttttaa tcgaaataaa gtctaagtgg tatagacaga gcaaaacagg
     5581 agggtgaaca gtggtgaaca gacggtgaac agtcatgccg tcaactgttc accctttatc
     5641 ttactgtatt tcttatattt ttatttaagg tgaacagtgg tgaatagtta taagtaaaaa
     5701 aacaaattga gagaaaggtt ttgctgagac ctttctctgg caagccgggt tttaagtgct
     5761 gtttgtgcca gaactgccac aactgcaatg aatcgagatg ttgtgtgatg aagggcagaa
     5821 tcatttcaag ttgaacacac ggagagcctg aacatgaaac ccgaaacagt catcaccgcc
     5881 ctgcagaatg tagccgccca acagtctgca gagagcagcc agcgcatcac atataagctg
     5941 agcgcattca ctgcggccag agacacgcac gcggccagca tgcaggaact gaaagagatt
     6001 gatacgtcca ttgaacgctg taagcaggag cggcagaccg ccctcagtga gagtgcagag
     6061 gcggagcagg actggcgcag ccgcttccgc accctgcgcg gcaatctcac ccctgaaatg
     6121 aaagctgagc acagcaagcg tatcgccagt cgcgagctgg ccgacgagtt caccggcctg
     6181 attgcggagc tggagactga ccggacacgc gccatgctga atgcctgctc caccggcaat
     6241 aaatacctgt cagcgcatga tgatgccttt accacttatg ccggtgcgga atgggctcag
     6301 gcggtcaaca cgctccccgc cgcgctcatt cgtgctttcc tgctgcgcat tcgtgccctc
     6361 gaaatgaagg gtgacagcgc accgcagtcc gtggccatcg gcgagctgcg cgatgcgctg
     6421 agccgtcagg gcagcctgta tcagttcgat atgaagcagg agccggtatt gtccgtgacg
     6481 ggcatgcacc ggccacagat taccggtgtg gatatggagt tgttacgcag cccggcaaag
     6541 agaatgatgc tcgccagaaa gctgtctgaa aatgacggca ctaaggtgga ggcataagta
     6601 tgtttcactg tccgttctgc aaaaccagtg cacacgcccg taccagtcgt tatctgtcag
     6661 agaacgttaa acagcgctat caccagtgcg tgaatatcga gtgctcggca actttccgca
     6721 cgcttgaatc cgttgacggg attatctgtc cgccgccgac agagccggtc attcctgcgt
     6781 ccgaaccggc agccaccgtt aaccgtgcgg gcgcttaaac acgaccagtc atcaggagaa
     6841 acttacgtga ccacactgat gctacagaaa gcctttgagg cctgtcaggc aaataaatcc
     6901 gcctggctgc aacgcaggga tgaactgaag caggccgaac aggcataccg cgaacagctt
     6961 gccggtaacg gccacagcgg ccggagcctg aaaatcctgc gcgaaattat cgacgtgaaa
     7021 aagtgggaaa taaatcaggc tgccgggcgt tatattcgct cgcatgagga ggtgcagcga
     7081 atcagcatcc gtaaccgtct gaatgatttt atgcaggtgc acggcgcgga gctggccgcc
     7141 gcccttgccc cggagctgat gaattattcc gggcaaaatt ccgccgttca gcgctgcgcc
     7201 atgcagcact cagtcgacta tcttcgtgag gcgttacagg tctggctgac cgccggtgaa
     7261 aaaattaatt attcggcgca ggataatgac attttaacgg ccatcggatt caggcctgat
     7321 gcggcttcgc gagatgatag tcgtgaaaaa ttcacgccag cacagaacct gaattacacc
     7381 cgccgccgtg cagaactggc cgcgcagtag tccgcttaaa gattcccgat aatcccgcca
     7441 tttttacgta aaaaagccat gcatgcataa ggtgcatggt tttgcatgcg ttttaccggc
     7501 gctggattcc ccgccagcgc cagcactggc gcgtcctgag gcctgtcatg cacctgcatt
     7561 aaatgcgccc ccttaagcgg gcaggcgggg cggggagagc attgcgcgct aaaactattt
     7621 tgataattta tcaaggatag ataagtactt gtcctcttcg aagctaaaac cgaatttttt
     7681 gaaggatttt agataattga aatgcccctt cagtgtgctt aacgttaatg ggtttcgatc
     7741 atttgacttt agcgcctgat caaaaattgc cctgactttt ctacgatatg cacgagttgg
     7801 tctaagggga ccgttaacta aaataccagt aacagcttgc ctgttattat atg
//
LOCUS       DAPGWD010000190         7737 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPGWD010000190 DAPGWD010000000
VERSION     DAPGWD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.191"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            333..914
                     /gene="mdaB"
                     /locus_tag="RHX88_003976"
     CDS             333..914
                     /gene="mdaB"
                     /locus_tag="RHX88_003976"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDV2232603.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            945..1259
                     /locus_tag="RHX88_003977"
     CDS             945..1259
                     /locus_tag="RHX88_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDV2232604.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1303..2634)
                     /locus_tag="RHX88_003978"
     CDS             complement(1303..2634)
                     /locus_tag="RHX88_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2232605.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDITNTRWGKYRPWLLFMSIPFGISIWLMFTTPDTDY
                     SVKLLWAWATYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGTMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLSGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAYLDYQPGV
                     EQTPQALWGICLLMTIIPAILNLLTGLIMRFYLINNEYYEQIKARLQTAEE"
     gene            2921..3673
                     /locus_tag="RHX88_003979"
     CDS             2921..3673
                     /locus_tag="RHX88_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2232606.1"
                     /translation="MIRIYKASKGQLKHREIFDEIKKKIDNGEWKEGKAIPTERELAE
                     HFLSSRTTIRKAIESLKQRGYLHSVHGQGTFTLPARSRENHLLHSFTDDIKARGGVPA
                     QNILEIGYIPLSDIIRKNLELDIHVHTVFCIKRIRYMGSTPLGIQTSWLALNDGQEIT
                     LEELLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            3687..4763
                     /locus_tag="RHX88_003980"
     CDS             3687..4763
                     /locus_tag="RHX88_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDV2232607.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKTQWGG
                     FLNAFVSPPADTFAGSLNGVAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWPGKKEKACRLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDVEAIASGLTLLAVQAQEK"
     gene            4763..5761
                     /locus_tag="RHX88_003981"
     CDS             4763..5761
                     /locus_tag="RHX88_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDV2232608.1"
                     /translation="MNMPARAPVMPLAQTREEINATAAALRHLAAIWGECFSSVQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCSALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSLTVDALSHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYTLLRLAQNLCPGLTPLQIEEDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATAFPLEEFYHSLRLHQAGVGQHYFLLPATSREYPFYIATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            5766..6680
                     /locus_tag="RHX88_003982"
     CDS             5766..6680
                     /locus_tag="RHX88_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDV2232609.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYYYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYAAEPDFTLLHDIQDEYQLEAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPIIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLATPSEVNLFLSQHD"
     gene            6710..>7737
                     /locus_tag="RHX88_003983"
     CDS             6710..>7737
                     /locus_tag="RHX88_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HDV2232610.1"
                     /translation="MKIRHAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDA
                     KQQPFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNIS
                     SDKESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSID
                     ADGRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVLALNDHQLLVIERSGRNVSAGFN
                     DWDYSVRVYMVDLTAASDIKDIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGV
                     TFGPLIDG"
BASE COUNT         2310 a         1685 c         1710 g         2032 t
ORIGIN      
        1 ttatgcttta acatatatca gtacgctcat aaaaatccat ttataattat caacatgaca
       61 cgttgatatt ttcatctttt acttattcaa agccatattt tcaatcccaa aaaaaaccga
      121 accaaacgtg taaattattc cggtgcgagt aagaattatg aggaatggct atcagtattg
      181 tcattttcag aaaatattta tcctgcatcg gtgagtcaga gcaagatcag acttttgcta
      241 aattcgcaaa agactttgca cattttgcta atttcaccgt accgctctgt gacgtactat
      301 agtcggcaaa cgtctcacct tgaggttaaa aaatgagcaa catcctgatt atcaacggcg
      361 cgaaaaaatt cgcccactcc aatggtcaac tgaacgacac cctgaccgaa gtcgcggatg
      421 gcacactgcg cgacctcggg catgatgtcc gcatcgttcg cgccgacagc gactacgatg
      481 tcaaagcgga agtacaaaac tttctctggg ctgatgtggt gatctggcag atgccaggct
      541 ggtggatggg cgcgccgtgg acagtgaaaa aatatattga tgatgtattc accgaaggtc
      601 atggaacgct gtatgccagc gatggccgta cccgcaaaga tccgtcaaaa aaatacggtt
      661 ccggcggcct ggtacagggc aaaaaatata tgctttctct gacctggaac gcaccaatgg
      721 aagccttcac cgaaaaagat caattcttcc acggcgttgg cgtcgacggt gtgtatctgc
      781 cgttccataa agcaaaccaa ttccttggca tggaaccgct gccgacattt atcgctaatg
      841 acgtgataaa aatgcctgat gttccccgct atactgaaga atatcgcaag catcttgtgg
      901 aaatttttgg ttaactagag ctcaggcttt agaaggagtt aaccatgctt accgtaatcg
      961 cagaaatccg tactcgtcct ggtcaacatc accgtcaggc ggtattggat cagtttgcta
     1021 aaatcgttcc aaccgtactg aaagaagaag gttgccacgg ctatgcgcca atggtggatt
     1081 gcgctgctgg cgtgagtttc cagtctatgg caccggattc tatcgtgatg attgagcagt
     1141 gggaaagcat cgcgcatctt gaagcgcatc tgcaaacccc gcacatgaag gcgtacagcg
     1201 aagccgtaaa aggcgacgtg ctggagatga atatccgtat tctgcagcca gggatttaat
     1261 atttctgaaa agaggccggt gctgcttgcc ggccatttat tttcactctt ccgccgtttg
     1321 taaccgtgct tttatctgtt cgtaatattc attattaatc agataaaatc tcatgatgag
     1381 tccggtaagc aggttaagta tcgcgggaat gatcgtcatt aacaggcaga tcccccacag
     1441 cgcctgtggt gtttgttcta cgcctggttg ataatccaga taggctaaag cgattccgat
     1501 aaagccccct gccagcccca taccgaactt ttggaaaaag agaatcccgc caaatgccag
     1561 gccagaaaca cgttgccccg ttttcatttc accgtaatcc acggactcag caatggatgc
     1621 ccaataaatg ggaagctgga tatccgtcag gaaagtcacc acaatataaa atgtaaaagc
     1681 tagcactata gaatttggtt gcacgaaata catcgcacca cccaccacga acgccagtaa
     1741 ttgggtataa cgaaatattt ttattttgtc atagagacga gtaagccagg tgcaagcgat
     1801 catagccaga actgaggcga tcacgcctga agttaaaaac ggtgaaatta aagcatcccc
     1861 accactcagg taatattttg cataatatgc agcgacagag ttacgaataa tcccgccaaa
     1921 cataatgatc gcaattacag cgcccaaaat gagccattga tcatttttga tcagtaaccg
     1981 aaactgcgcg gccataccga gactgggttt tggatggtag atgcgctctc tcacgctaaa
     2041 aaaacaatac aggcataaac acgttgccag aatacccatt gtccccattg ctatttgata
     2101 acccagttgt acgttattct tcccaagata cattgcagat aaaggaacaa taattgttac
     2161 ggcgaacatg gcaatttttg tcataacaaa acgataacca ttggcgctca atctctcttg
     2221 tggatcatcc gtgataacgc tgattaatga aacataagga atagcgatta acgtataggt
     2281 caaggtaagt aacacatagg tagcccaggc ccataaaagc ttaactgaat aatcggtatc
     2341 aggtgtggtg aacatcaacc aaatgcttat cccaaacggt attgacataa aaagcaacca
     2401 tggccgatat tttccccaac gtgtattcgt tatgtctgtc attgttccca caactggatc
     2461 aatcacagca tcaagaatac gtacagaaaa taacagaata cctaagtcga cgggtttcag
     2521 accaaataca tccgtgtaaa aataagtgat gatcatggct aaagacatca ttactatcgc
     2581 aatggccata tcaccggcac caaaacctat cttttcattt attgtcagtt tcatacacag
     2641 cactcattaa tcacatgaaa ttatattaaa aacaaatgta tcggaactca ttcagcacat
     2701 aactttacga ttgagaataa atgcattggt agataccaca acaaaccact gagttaatat
     2761 tgagcagcat cacataaaaa ccaatttcac ttaaccgcat gatatttatt aataaatgtg
     2821 gtttttcctg ttttatgtca cagataacaa ataagcatac tgcgcaataa aaataaaaga
     2881 atgtaaagtt ggtatcaacc acttgaaatg aggctttgtc atgataagaa tttataaagc
     2941 cagtaaaggt cagctaaagc atcgtgaaat ttttgatgaa ataaagaaaa aaattgataa
     3001 cggtgaatgg aaagaaggca aagctatacc aacagaaaga gaactggctg aacatttttt
     3061 aagcagcaga acaacaattc ggaaagcaat agaaagtttg aaacaacgcg gatacctgca
     3121 tagtgtccac gggcaaggaa catttacatt accggctcgc agtcgggaaa atcatttatt
     3181 acatagtttt actgatgaca ttaaggctcg tggtggcgta ccggcacaga atattcttga
     3241 aataggttat attcctctca gtgatattat cagaaaaaat ctcgaacttg atatccatgt
     3301 ccataccgtt ttctgtataa aacgcattcg ctatatggga agtacgccgc tgggtattca
     3361 gacatcatgg cttgcgttaa atgacggaca agaaataact ctggaagagc tgctggcgac
     3421 gggttcgctc tatattttac tggaagaaaa actcgctatt aaacctttag aagcaacgga
     3481 gattatctct gcccggcttc cctcgcccct tgagcgtcaa ttactggaac tgactgccga
     3541 tgacgtcgta ttaagttgta cccgcgtcac gttatccgtg gaacgtaaac caatggagta
     3601 tgtagagatg gtttatcccg caagtcggta ctcctacaaa gcacgaatta caaaagacag
     3661 ttttagcgtg taacagagag aatattgtga acaaaataga acaagccgta atttataaca
     3721 aaatattagg ttctttagcc tgcgcaggta tgggcgatgc attaggcgca gcgactgaac
     3781 tctacagcat tgatgaaata aaaactcaat ggggaggttt tttaaatgcc tttgtttctc
     3841 ctcctgccga tacctttgct ggctcattaa atggtgttgc aggacttatc accgacgatt
     3901 ccagccaaat gtatgtgttt tcagaagggt taattgaggc tggatttgat aacttcacca
     3961 ataatgactg gttagcttgt cttctgcgtt gggccgatat gcagccctac gcaaattata
     4021 aaggccccac aacagaacaa atcgtcaagg cactaaaaga aggtagacca acaaacacca
     4081 ttgggcgtat tggaacatct tctcgtcagg cccccaatgt cggcaccacg aatggcgcag
     4141 gaatgcgtgt agccccggca ggattgatct ggcctggcaa gaaagaaaaa gcgtgtcgcc
     4201 tggcgttaat aacttgccta ccttcacatg acaccaatat tgcgattgcc agcgcttgcg
     4261 ccatcgctgc agcgacatcg caggctatgt taccagaagc ctcgctcaca tcattactgg
     4321 atgccgcaat ctggggagcg aattacggcg aacgcctggc caaacaatat gcacgatgtg
     4381 tagcagggcc atctatcgcc atgcgcatcc aacttgcagc ggacatcgcc agacgtgcca
     4441 atgatctgga gtcttgtctg cgagaaatgg aaggtttggt tggtaactct gttgccgccc
     4501 atgaatccat tcctgcggca atcggtctat tgctgtactg caaaggagaa ccctgggaaa
     4561 ccatccatgc ctgcgccaat attggcaatg acaccgatag catcgctacg atggcaggcg
     4621 caattgcagg ggcatggcgc ggttttgatg cgctccctga agataaatat gcctttttcc
     4681 gcgccgtaaa taataaagat ttcgatgttg aagcgatcgc atcgggtctg acattgcttg
     4741 cagtacaggc acaggagaaa taatgaacat gccagcccgt gctcctgtta tgccattagc
     4801 gcaaactcgt gaggaaataa atgcaacggc ggcagcgcta cgccatttgg cagctatctg
     4861 gggtgaatgt ttttcatctg ttcagctcga taaacgtgct gttgaacgcg tctgtatagt
     4921 gggatcgggc gactcctgga cggtggcact atgtgccgcc gcctggctgg ggaaatacac
     4981 acatctgttt tgttccgcat tgcaaacgtg ggattttctt cagactgatt taacccgtta
     5041 tcagaaagaa acgttgatca ttattctcag cgcaagtggc agaccatcac tgaccgttga
     5101 cgcccttagc cacgcagtat gctcaaacgc gcaagtgctg ggtgtgacga attgccctgg
     5161 aacaccattt tgcgcaataa cagaaaacat gctctataca tgggcgaata agcagggtat
     5221 tccaacgcaa agtagtagcg tcacacttta taccctcctg cgcttagcgc aaaacttatg
     5281 tccaggctta acaccactgc aaattgaaga ggatttagaa gggaaattta gccagattaa
     5341 tcagcactgg cagcaaaaga agcgccattt ttaccaacaa aaagagataa cgtttctggg
     5401 gagtggctta agttggggac tagcaatatc ggggagcaat ttactttctt gcggcccaca
     5461 gattcgtgcg acagcctttc cactggaaga attttatcat tcattacgct tacatcaggc
     5521 tggtgtcggt cagcattatt ttttactgcc agctacatcc cgcgagtacc cattttacat
     5581 tgcaacccaa aaagcaattg ttgaacaggg agcgacagca gaattaatca gttttatacc
     5641 tgatgccagt gagtccaaca atttatttct ggtcatgcag tggttatatg aaatgtgctg
     5701 gcatttaagt tgtgattatg tcgacgcagg cgggcaacgc gttagccata gggagaaata
     5761 agactatgaa gaaatttgat gtcgccgctt taggcagtgg caatatagat atttttttat
     5821 ctatcccgtc actccccact cgtggcggta aagtggtcgg gacacgtctg ggtgaacaag
     5881 ctggcggcac agtcgccaac agtgcctgtg cgatgggaca actgggtctc aatgtggtat
     5941 ctgtttcatg tgtgggcaat gatcattcag cctcgatcat tcttgatgga tttaaaaaat
     6001 atcatgtaaa ttgtgatttc gttcaggtaa ttccggaatt gattgccaat accgcgatta
     6061 tctttattga tgaattaggt gaaaaaacgt tggtttactc gcctggtagt gatcatgagt
     6121 gggataaaga aaaagccctg caggcaattg cgcaaagccg ttattattat acgatgccag
     6181 cagatatcga gaaatttcgt atgctggccg aatattcccg tagccagatg acaaaagttg
     6241 tcgttgatat tgagcctcat atcattgcaa cacctgaaca ccttgccaga attttacacc
     6301 ttgctgatat tgcgatattt aattatgacg gttttattcg tggctatgca gcagagccag
     6361 attttacact tctgcatgat atacaagacg agtatcaact tgaggctgtc gtggttacgc
     6421 tggatgttag gggagtaatt gcagtcaaag gcaatgagca agtaaaaata ggtagctata
     6481 caattcccat tattgatacc actggcgctg gagatacttt taatggtgca tttgtttata
     6541 gcttaataaa aaacatgccc ttaattgaag cactaaaatt tgcttctgcc actgcagcaa
     6601 taaatattac tgcattaggt gccagaggtc atctggcgac accatcggaa gttaatcttt
     6661 ttctttcaca acacgactaa aatcagatac atacacaaag gtaaatatca tgaaaataag
     6721 acatgccgct atttttctgg cattaccgct gttatgcaca acagcgcagg caaacagttt
     6781 tcttcattat gctggtgagt tcaacttccg gacgggagaa aaacagaaca atgttacaat
     6841 cgcggggcta tcagccatca catttgatgc aaaaaaaaat attttctatg ctattaacga
     6901 tagcagaaac aataataaag aaggagatgc cagtctttat acactaaaaa tacaggtatc
     6961 atccaggggc attgagcaag tgaattttct caaccagcgt ccactgcttg atgcaaaaca
     7021 gcagcctttc gtgaccaata cggtggatgc cgaaggtctg gccctgacac acaatggtaa
     7081 gtcattacta tggagttcgg agttaggtgc tccactgcgt ctgagcactc tcgatggcgt
     7141 aatggagaaa gattttacgt ctttatttcc tgctcgattt aatattagtt ctgacaaaga
     7201 gagcagcaat ggtattcgca gcggcaacgc ctgggagggc ctgacagtta caccagatgg
     7261 aaaatcactt tttatcgccg ttgaaagcag ccttaaacag gatgggccta ttgcaagtcc
     7321 aataaattca ggtacatcac gcttactgca attttctata gatgcagacg gtcgaccttc
     7381 aaagcaattg catgaatatc tctatattac tgatcctgtt ccgcaagtga gtaagttcgg
     7441 tattaatgat aatggcgtat ccgaggtcct ggcgctgaat gatcaccaat tgttggtcat
     7501 cgaacgttcc ggcagaaacg tcagtgctgg gttcaatgac tgggattact ctgtcagagt
     7561 ttatatggtc gatttaactg ctgccagcga tatcaaggat attgacagtc tgcaagattg
     7621 gtccaataaa tccacgttac aacccgtcag taaaaaatta ttaattgatt ttgctgatta
     7681 cacttcctct gcggattgta ttgaaggggt aaccttcggt cctctgattg atgggca
//
LOCUS       DAPGWD010000191         7564 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPGWD010000191 DAPGWD010000000
VERSION     DAPGWD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.192"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..221
                     /locus_tag="RHX88_003984"
     CDS             3..221
                     /locus_tag="RHX88_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDV2232611.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            440..682
                     /locus_tag="RHX88_003985"
     CDS             440..682
                     /locus_tag="RHX88_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232612.1"
                     /translation="MNSLVGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1012..1941)
                     /gene="fdhE"
                     /locus_tag="RHX88_003986"
     CDS             complement(1012..1941)
                     /gene="fdhE"
                     /locus_tag="RHX88_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDV2232613.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1938..2573)
                     /gene="fdoI"
                     /locus_tag="RHX88_003987"
     CDS             complement(1938..2573)
                     /gene="fdoI"
                     /locus_tag="RHX88_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV2232614.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2570..3472)
                     /gene="fdoH"
                     /locus_tag="RHX88_003988"
     CDS             complement(2570..3472)
                     /gene="fdoH"
                     /locus_tag="RHX88_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDV2232615.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3485..6535)
                     /gene="fdnG"
                     /locus_tag="RHX88_003989"
     CDS             complement(3485..6535)
                     /gene="fdnG"
                     /locus_tag="RHX88_003989"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5948..5950),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV2232616.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6729..7562
                     /gene="fdhD"
                     /locus_tag="RHX88_003990"
     CDS             6729..7562
                     /gene="fdhD"
                     /locus_tag="RHX88_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDV2232617.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1723 a         1966 c         2038 g         1837 t
ORIGIN      
        1 taatggcgat gaatacggtt tttcttcatt tatcagaaga ggcaattaaa cggctgaaca
       61 aacttcgggg ctggcgtaag gtttcacgat ctgcaatttt acgcgaagcg gttgagcaat
      121 atctggagcg gcagcaattt ccggtgcgta aagcaaaggg aggcaggcaa aagggggagg
      181 tagttggtgt tgatgatcag tgtaaggagc ataaggaatg aaaaaaattt ttcattcttg
      241 aatataaaaa aacagatgcc cttattctgg tattaataca aggctgtttt acttgaactt
      301 ataataactg caactgttac atcgtatctg gaaaacgcct cgcaaaaata aaaaatgatg
      361 cgtaaatgag ctttctttaa agattcggca agggtacttt tctctccata gattcaaaaa
      421 tgatattggc gagatattta tgaactctct ggtaggtata gatatgggca gaattttact
      481 cgatttatcg aatgaggtga ttaagcaact tgatgatctc gaggtgcagc gtaatcttcc
      541 tcgtgcggac ctattaaggg aagcggtaga tcaatacctg ataaatcaat cgcaaacagc
      601 aagaaccagt gttcctggca tctggcaagg gtgtgaggaa gatggtgtcg aatatcagcg
      661 taagctgcgc gaggaatggt aatgggaaaa aatgatgtca atcagattgc tgacaacgtg
      721 cgcgttgttc atgccggatg cggcgtgaac gccttatccg gcctacaaag tcatgcaaat
      781 tcaatatatt gaagtttatt ggtagggctg ataagcgcag cgcatcaggc aatcatacgc
      841 ttgtcatcag tgacataccc ataaccttat cgatgtgttg gagggaagaa cgaggcaaac
      901 agtcaattgc ggcatgatct tgaatgggta acgcggaata cgagagatgg tgtcgtcaca
      961 ccctatgagc tgtaatccgg gcgaccagcg ccacccggaa aacgtcagag attactcccc
     1021 ttcacccgga aacaggaacg ggttgatgga actgcgggca tagccttctt gctccattcg
     1081 cgcgtccagt accagagaag cgaggtcatc tgccacggct tcaattttcg gttctttttc
     1141 ctgataaaga atcttcaggt aagtgccaca gtcatcgcag ctttcggctt taatcgcggc
     1201 ctgttcgtca tccagcgacc agtaatgcag tttgccgctc tgctcgcagt tgctgcattt
     1261 tacgcgcact acgtgccatt cggtttcaca caggttgcag tgcaggtaac gcagaccctg
     1321 agtggtgcca atttgcacca tgctggacac cggcatagaa ccacaaaccg ggcaatattg
     1381 acgttgctcg ccgtattcag cgcgggcttt gccggggatc agattggcca tctgcgccca
     1441 gtagagcgac agtgcagccc agataaacgg cgctttatcg ctgctgacgg atgagaaatc
     1501 agaggcaaac agtgcgctgg ccatatcttc cagctcctga gttgatgcct tctccagatt
     1561 ctcaatcact gccagcgccg ggccgctcat ttcaggtttc agctcagcaa tcagcgccat
     1621 cagcagcttt tgccagtgct tatcacgcgg cagaacgtga atatccagcg ggggcttgcc
     1681 ctgtgcgctg gcttctttaa tgcgcgcggt cagatccatc tccagcggat ggtcgtacag
     1741 caccacttcc tgggcgtggg cgataagcgc agcaaagcgc aggtaatcac ccagcggatt
     1801 attttctgcc agctcgcgca gacgctcggc gcggcggttg tataaattct tgagccgagg
     1861 gaacaataac ggcggaatca tatccgccgt acgtttctcg ctcgaaccca gctcatcttg
     1921 cgggattatg cgaatactca ttcagctttc ttttccgttg tcttgcggac ctcacggtac
     1981 cagcgcgggt gatgtttctt cgcccaggcg ctggtaaccc atccttccac catggcggta
     2041 atcgtgcctt tcacccaaag ggcggcgtag atatgcacca tgataaccac aattaacgcc
     2101 actgcggcaa atgaatgcag cattaacgcg aatcggatca ccgggattga gaaagcaggc
     2161 gcaaaataag gacgccagat aatcacgccg ctcaccagca gcagaaccag gaaaataatc
     2221 gccgcccaga aaacgcattt ctgaccgaag ttataacgcc cggtgtcacc tacttcctcg
     2281 ttgacgacga tcttacgaat attcttcgcc caaaagatat catcccgatt gattaggttg
     2341 tgatgccagt aacggaaaaa catgatgatg aacgaggcaa acataaccac gccgacaaac
     2401 gggtgcagaa ttcgcgccag ctgcggtgtg cccatgattt gcatcaacca gttgaaggac
     2461 gggaacaaaa agcccagccc gctcactgcc gccaggatga agcagaaggc ggtgatccag
     2521 tggttgatac gttccggcgc ggtgtagcgc acgatggtgt cacgtcgttt catttgcgct
     2581 cctcgtcttt ctcttcgtgc agattattct cttcctcatc cgcacggttc ggaccgacac
     2641 ccacgtagtg gaagatactg gctgcgaagg tagccgcaaa gccaacagct gcgagcggtt
     2701 tccagatgcc tttccagaat ttcacggttt cgctgatttc cgggttctcc ggcaagccat
     2761 gatacagatt tggcttgtca gcatggtgca gcacgtacat aacgtgtgta ccaccgacgc
     2821 ctgccggatc gtacaggccc gcattgtcgt aaccgcgggt tttcagctcc gccacgcgct
     2881 cgctcgccag cgttttcatc gactctttcg taccgaagtg aatcgcgccc gttgggcagg
     2941 tcttcacgca ggctggttct tgcccaacca ccacgcggtc aacgcacagc gtacatttgt
     3001 agacgcggtt gtcttccggg ttgaggcgcg gaatgtcgaa cggacagccc gcaatgcaat
     3061 aaccgcagcc aatgcactgc tcggactgga agtcgacgat accgttggca tactgaataa
     3121 ttgcaccttc cgccgggcac gctttcaggc agcctggatc ggaacagtgc atacagccgt
     3181 ctttgcggat cagccattcc agtttgtcgt tctgctccac ttccgagaag cgcattaccg
     3241 tccacgattt ggcgcttaaa tcattggggt tgtcgtacac cccaatgtta ttgccgacgg
     3301 tatcgcgaat gtcgttccac tctgaacacg ccacctgaca ggctttacag ccgatacagg
     3361 tggtgacgtc gatgagtttc gccacttctt cctggaagtc ccgcgcctga ggcgcggggg
     3421 tcagaccgtt agtcgcggaa cgacgaatga tatcttgaga ttgataagcc atatgtcgtc
     3481 tccgttacac cttttccaca ttcacaagga aggacttaaa ctccggcgtc tgcgtgttcg
     3541 catcaccaac gaatggcgtt aacgtattgg caataaagcc ttttttagct acgccttcat
     3601 agccccagtg aatcggaata ccgatggtat cgatatcttt gccgttcgct ttcagtgtac
     3661 gaatacgttt ggtcaccacc gctttggcct tgatatagcc acggttggag gagactttaa
     3721 cggtatcgcc ctgggcaatg ccaagtttat tcgccagcga ctccccgatt tccacaaact
     3781 gctctggttg caaaatcgcg ttcaacagcg cgtgtttggt ccagtagtgg aaatgctcgg
     3841 tcagacgata ggtggttccg acatacggga acttatcggc tttacccaat gcttcggcgt
     3901 cgtctttaaa gatacgtgca gccgggttcg agataacgtt tggatgcagc gggttagttc
     3961 ccagcggcgt ttcaaacggc tcgtagtgtt ccgggaacgg accttccgcc atcttatcga
     4021 gggcaaacag acgccccatg ccttcctgct gcatgataaa cggcccgacg ccgctacccg
     4081 gaggcgctgc gctgtagtcc ggaatatccc agccggtcca cttagtgccg tcccatttca
     4141 gcaactgacg cttcggatcc cacgggttac cctgcggatc tgcggaggcg cggttataca
     4201 gaatgcggcg gttaagcggc catgcccatg cccagcccag cgtgttaccg aggccagacg
     4261 gatcggcgtt atcacgacgc gccatctggt tgccttccgg cgtccagcta ccggcgaaaa
     4321 tccagcagcc acaggaggtt gtaccgtcat cgcgcaattg ggcgaacgag ctaagttgtt
     4381 ggcctttctt gacgataacc gcaccggttg ccggatcggt aatatcggcc agcgccttac
     4441 cgttgctctc catcgctact tcttccgact tcggctcatg cggaatggcg tagttccagg
     4501 tcatgttcag cacctgatcc gggttcgcgc caccctgttc ggcatacatc ttgcgcaagc
     4561 gcaggaagat accggagagg atctcaccgt cagtcagcgc aatccccggg gcgtccgcac
     4621 ctttccagtg ccactgcaac cagcgacctg agttaacgat tgaaccgttc tcttctgcga
     4681 agcaggtcga tggcagacgg aacacttcgg tctggatctt cgacgagtca acttcgttca
     4741 gctcaccgtg gttctgccag aagttagagg tttcagtgtt cagcgggtcg atggtgacga
     4801 ggaacttcag tttcgacaga caaccgatca ctttgttttt gttcgggaat gaggcaacag
     4861 ggttaaagcc ctggcagata tagccattga ccttgccttc tttcatcatc tcgaagtatt
     4921 gcaggacgtc gtagccttta tcccacttcg gcaaccagtc aaagccccag ctattttccg
     4981 ccgtcgcttt atcaccaaag aaggccttca tcatagagac gaagaatttc gggtagttgc
     5041 cccagtagtt tacctggcct tccagcagcg gttttggcgt gttggcggta aggtaggttt
     5101 gcagatcggt ctgcttctcg cttggcagcg tcatgtaacc tggcagactc tgcgacagta
     5161 gccccaggtc cgtcagcccc tgaatattgg agtgaccgcg cagggcgtta acgccgccgc
     5221 cggccatccc catattgccg agcagcagct ggatcatcgc catcgtacga atgttttgcg
     5281 caccaacgga gtgttgcgtc cagccgaggg catacaggaa cgaggcagtt ttatcgtgag
     5341 cactggtttc tgcgatgtat tcgcagactt tcaggaacgc gtcttttggc gtaccacaga
     5401 tgttttcaac cacatctggc gtgtagcggg aaacgtgctg tttcagcaag ttccacacgc
     5461 agcgcggatg ttgcagcgtg gtatcgcgtt tggcgaagcc gttttcgtcc agttcataag
     5521 tccaggagga tttatcgtac ttgcgttttt ccgcgtcgta gccggtgaac aggccatctt
     5581 caaagccgta atcctcacgc actatcaggc tggcgttggt ataggcttcg gtgtattcgc
     5641 ggttgaattt ttcattgttc agcaggtaca gcaatacgcc tgacaggaaa gcaatgtcag
     5701 taccggaacg aataggggca tagtagtcag ctaccgccgc cgtacgcgta aagcgaggat
     5761 cgatcacaat cagcttcgcg ccgttgtgaa ttttggcttc catcgcccag cggaacccga
     5821 ccgggtgagc ttcagcggcg ttaccgccca tcaccacgac gaggttggcg ttcttgatgt
     5881 cgacccagtg gttggtcatc gcaccgcgac caaatgttgg agcaagactt gctaccgttg
     5941 gtccgtgtca gacacgcgcc tggttgtcga ccgcgagcat acccagcgcg cgggagaatt
     6001 tttgcgttaa atagccggtt tcgttactcg acgcggaagc acacagcatc ccggtggaga
     6061 gccagcggtt aacagtcacg ccttcggcgt tttgcgcaat gtagttagca tcgcggtctt
     6121 ctttcatcag tttggcgatg cgatcaaacg cctcttccca actgatttgc tgccatttat
     6181 cagaacctgg cgcacggtat tccggaaact tcagacggct ttcggagtgg atgaaatcca
     6241 ccaggccagc gcctttcgga caaagtgcac cgcggttgac cgggtgatcc ggatcgcctt
     6301 cgatatggaa gatagatgct ttggcgtttt ttgctccgtc accgaggctg tacatcaaca
     6361 gcccacagcc tacggaacaa taggtgcagg tattacgggt ttcgcgggtg cgcagcagtt
     6421 tatactgccg ggtttccgcg agcgctacgc tgggtgcaaa acccagtgcc gctgccgtgg
     6481 tgcctgccat accgccagcg cagatcttaa agaactgcct tctgctgacc tgcatggatt
     6541 gctccttgtt tcgacattgt cacgtcccat ttacattcgc ttgctgcgtg tgcagggagt
     6601 gggagttatt tttctttgcg gaaggggccg caaaggtcca gaattggctc aatttccctc
     6661 catccaggaa gggtttgtaa cagaatacca taatgttggt gtgtgtgttc ttatctggtt
     6721 aagagaaagt gaaaaaaaca cagcaaaaag aaatcgaaaa tgtgacaaat atcacaggtg
     6781 ttcgtcaaat tgagttatgg cggcgtgatg atttacaaca cccacggtta gatgaggtcg
     6841 cggaagaagt tcccgttgcg ctggtctaca acggcatttc gcatgtggtg atgatggcgt
     6901 cgcccaaaga ccttgagtac tttgcgctcg gtttttcgct ttccgagggg ataatagaaa
     6961 gtccgcgcga tatcttcggc atggatgtcg ttccttcctg taatggtctt gaagtacaaa
     7021 ttgagctttc cagccgccgc tttatggggt tgaaggagcg ccgccgggcg ctggcgggac
     7081 gtacgggctg tggcgtatgc ggcgtggagc aacttaatga catcggaaaa ccggtgcagc
     7141 cgctaccgtt cacccagacg tttgatctca acaaactgga tgatgcatta cgtcatctca
     7201 acgattttca gccagtgggg caactgactg gttgtactca cgccgctgcc tggatgttgc
     7261 catctggcga actggtcggc gggcatgaag acgtgggtcg ccatgtggcg ctggacaaac
     7321 tgttaggccg ccggtcacaa gaaggggaaa gctggcagca aggtgcggta ctggtttcca
     7381 gtcgtgccag ttatgaaatg gtgcaaaagt cggcgatgtg cggcgtagag attttgtttg
     7441 cggtgtctgc cgcgaccacg cttgctgtag aagtggctga gcgttgtaat ctgacgctgg
     7501 tgggtttttg taagccgggt cgggcaacgg tttataccca tccgcagcgt ttgagcaatt
     7561 aatt
//
LOCUS       DAPGWD010000192         7544 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPGWD010000192 DAPGWD010000000
VERSION     DAPGWD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.193"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            14..868
                     /gene="gatY"
                     /locus_tag="RHX88_003991"
     CDS             14..868
                     /gene="gatY"
                     /locus_tag="RHX88_003991"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDV2232618.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            897..2159
                     /gene="gatZ"
                     /locus_tag="RHX88_003992"
     CDS             897..2159
                     /gene="gatZ"
                     /locus_tag="RHX88_003992"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDV2232619.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDTPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2169..2621
                     /gene="gatA"
                     /locus_tag="RHX88_003993"
     CDS             2169..2621
                     /gene="gatA"
                     /locus_tag="RHX88_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDV2232620.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2652..2936
                     /gene="gatB"
                     /locus_tag="RHX88_003994"
     CDS             2652..2936
                     /gene="gatB"
                     /locus_tag="RHX88_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDV2232621.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            2940..4295
                     /locus_tag="RHX88_003995"
     CDS             2940..4295
                     /locus_tag="RHX88_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDV2232622.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            4343..5383
                     /gene="gatD"
                     /locus_tag="RHX88_003996"
     CDS             4343..5383
                     /gene="gatD"
                     /locus_tag="RHX88_003996"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV2232623.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            5489..6262
                     /locus_tag="RHX88_003997"
     CDS             5489..6262
                     /locus_tag="RHX88_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2232624.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6344..7243)
                     /gene="yegS"
                     /locus_tag="RHX88_003998"
     CDS             complement(6344..7243)
                     /gene="yegS"
                     /locus_tag="RHX88_003998"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDV2232625.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1908 a         1693 c         1951 g         1992 t
ORIGIN      
        1 acaggatatg aaaatgtacg tggtatcgac aaagcagatg ctgaacaacg cacagcgcgg
       61 cggttatgcg gttccggcat tcaatattca caatctcgaa acgatgcaag tggtggtgga
      121 aaccgctgcc aacctgcatg caccggtcat catcgccgga acgcctggca catttactca
      181 tgctggtaca gaaaatctgt tggcgctggt cagcgcgatg gcgaagcaat atcaccatcc
      241 actggcaatt catctcgacc atcacacgaa atttgacgat atcgctcaga aggttcgttc
      301 tggcgtgcgc tcagtcatga ttgacgcctc gcatttgcct tttgcgcaaa atatttcacg
      361 ggtcaaagag gtggtggatt tttgccatcg ctttgatgtc agcgtcgaag cggagctggg
      421 gcaacttggc ggccaggaag atgatgtgca agtcaatgaa gccgatgctt tttataccaa
      481 ccccgctcag gcgcgtgaat ttgccgaggc aaccggaatt gattccctgg cggtcgccat
      541 cggcacggct catgggatgt atgccagcgc accggcgctt gatttttctc gactggagaa
      601 cattcgccag tgggtgaact taccgctggt gctgcatggc gcgtcagggt tatcgactaa
      661 ggatattcag caaaccatca aactggggat atgcaaaatc aacgttgcaa cggagctgaa
      721 aaatgccttc tcgcaggcgt taaaaaatta cctgaccgag caccctgaag cgaccgatcc
      781 ccgggattat ttgcagtcgg ctaaatccgc aatgcgcgat gtggtgagca aagtgattgc
      841 cgattgtggc tgcgagggca gggcataacg cacctgccat ttaacaagga aaaaacatga
      901 aaacgttaat tgcccggcat aaagctggtg aacatatcgg catatgttca gtctgttctg
      961 cccatccgtt ggttatcgaa gcggcgctgg catttgatcg caacagcacg cgcaaagtgc
     1021 tgattgaagc aacgtcaaac caggtcaatc aatttggcgg ttataccgga atgacaccgg
     1081 cagactttcg cgaatttgtt tttacgattg ccgataaagt tgggtttgca cgcgaacgca
     1141 ttattctcgg cggcgatcat ctggggccaa actgctggca gcaagaaaat gcggatgcgg
     1201 cgatggaaaa atccgtcgag ctggtaaaag catatgttcg tgctggcttc agtaaaattc
     1261 atcttgatgc gtcaatgtcc tgcgcggggg atcccatacc gttagcacca gaaacggttg
     1321 cggaacgagc tgctgtgctt tgctttgctg cggaaagtgt ggcgacagat tgccagcgtg
     1381 agcaactgag ctatgtcatt ggcaccgaag ttccggttcc gggcggtgag gccagcgcca
     1441 ttcagtcagt acacatcacc catgttgaag atgccgccaa tactttacgt acgcatcaaa
     1501 aggcctttat tgcccgtggg ctgacagagg cgttaacacg tgtgattgcc atcgtggtgc
     1561 agccgggtgt ggaatttgat cacagcaata ttatccatta tcagccgcag gaagcgcagg
     1621 cgctggcgca atggatagaa aacacccgaa tggtttatga agcacattct accgattacc
     1681 agacccggac ggcttattgg gaattagtcc gcgatcactt tgcaatattg aaagtcggtc
     1741 ccgcattaac ctttgcttta cgcgaggcga tatttgcact ggcacaaatt gagcaggaac
     1801 ttatcgcccc cgaaaatcgc agcggttgcc tggcggtaat tgaagaagtg atgctcgacg
     1861 aaccgcaata ctggaaaaaa tattatcgta cgggttttaa cgattcatta ctggatattc
     1921 gttacagcct gtcggatcgt attcgttatt actggccgca tagtcggatt aaaaatagcg
     1981 tcgaaacgat gatggtgaat cttgaaggcg tggacacccc actgggcatg attagtcagt
     2041 atcttcccaa acaatttgaa cgcattcagt ccggggaatt atcagcaata ccgcatcagc
     2101 tgattatgga taaaatttat gatgttttgc gcgcctatcg ctacggctgt gcggaataag
     2161 gacggtatat gactaacctg tttgttcgta gcggaatttc ttttgtcgat cgtagcgaag
     2221 ttttaaccca tatcggtaat gagatgctcg ccaaaggtgt ggttcatgat acctggccac
     2281 aggcattaat tgccagagaa gcagaattcc ctaccgggat aatgcttgag cagcacgcca
     2341 ttgcaatacc gcattgtgag gcgattcatg ctaagtcgtc agccatttat ctgttaaggc
     2401 caacaaataa agttcatttt cagcaagcgg atgatgataa cgacgtggag gtatcgttgg
     2461 ttattgcgtt gattgtggaa aatccgcagc agcaattgaa acttttacgc tgtttatttg
     2521 gcaagttaca acagcccgat atcgtcgaga cactaatcac tcttcctgaa acccagttaa
     2581 aggaatactt cacaaagtat gttttagatt cagacgaata aatccctctg taacaataaa
     2641 aaggactatt tatgaaacgc aagattattg tcgcttgcgg aggcgcggtt gcgacctcta
     2701 cgatggcggc ggaagaaatt aaagagttgt gtcagaatca taatattcct gttgaattaa
     2761 tccagtgtcg ggttaatgaa atagaaacct atatggatgg tgtgcatttg atatgcacca
     2821 ctgccaaagt ggatcgtagt tttggcgata ttccgttagt tcacggcatg ccttttattt
     2881 ctggtgtcgg tatcgaagca ttacaaaata aaattctgac tatcttacag gggtgaccta
     2941 tgttttcaga agtcatgcgt tatattctcg acctcggccc tacagtgatg ctgccggttg
     3001 tcatcattat tttttctaaa atattaggca tgaaggcagg cgattgcttt aaagcgggtc
     3061 tgcatatcgg gattggcttt gttggcattg gccttgtgat tggcttaatg ctggattcca
     3121 ttggtccggc ggcgaaagcg atggcggaaa atttcgacct gaatctgcat gtggtcgatg
     3181 tcggctggcc gggctcttca ccaatgacct gggcgtcgca aattgcgctg gtggcgattc
     3241 cgattgcgat attggttaac gtggcgatgt tactgacccg tatgacgcgg gtggtaaatg
     3301 ttgatatctg gaatatctgg catatgacct tcaccggcgc gttgctgcac ctggcaaccg
     3361 gttcatggat gatagggatg gcaggtgtgg taattcacgc ggcgtttgtt tataagctcg
     3421 gcgactggtt tgcccgcgat acccgaaatt tctttgagct ggaaggtatt gctattccgc
     3481 acggtacgtc ggcgtatatg gggccgattg cggtgctggt cgatgctatc atcgagaaaa
     3541 tcccaggcgt taaccgaatt aaatttagcg ccgacgatat tcagcgcaaa tttggccctt
     3601 ttggcgaacc tgtcaccgtg ggttttgtga tggggctgat tatcggcatc ctcgcgggtt
     3661 acgatgtcaa aggtgtattg cagctggcgg taaaaacggc ggcagttatg ctgctgatgc
     3721 cacgggtgat taaacccatc atggatggtt taacgcccat cgctaagcag gcccgtagtc
     3781 gtttacaggc gaagttcggc ggtcaggagt tcctgattgg cctggatccc gcattactgc
     3841 tggggcatac ggcggtggta tcggcaagcc tgatttttat cccactcacc attttaattg
     3901 ctgtttgtgt tccgggtaat caggtgctgc cgtttggcga tcttgccacc atcggcttct
     3961 ttgtggcgat ggcagtcgcc gtgcatcgtg gaaatctgtt ccgcacctta atctcgggtg
     4021 tcatcatcat gagcatcact ctgtggatcg cgacgcaaac tattggtttg cacacccaac
     4081 tggcggctaa tgctggggcg ttaaaagccg ggggtatggt ggcttcaatg gatcagggcg
     4141 gttctcccat tacctggtta ctgattcagg ttttctcccc gcaaaatatt cccggattca
     4201 ttattatcgg cgcaatttat cttaccggta ttttcatgac ctggcgtaga gcgcgtggct
     4261 ttattaaaca agagaaagtc gttctcgcag aataattttt acctgagggg gaggttatcc
     4321 cccttaacaa tcaggagttt ttatgaaatc agtggtgaat gatactgatg gtatcgtgcg
     4381 cgttgcagaa agcgtcattc ctgaaattaa acatcaggat gaggtgcggg taaaaattgc
     4441 cagctcgggc ttatgtggtt ccgatttacc caggatattt aaaaatggtg cacattatta
     4501 tccaataacg ttaggccatg aatttagcgg ctatattgat gcggtgggat ccggtgttga
     4561 tgatttacat cctggcgatg cggttgcctg tgtgccgtta ttaccctgtt ttacttgtcc
     4621 agagtgtttg aaagggtttt attcccagtg cgcaaaatat gattttattg gctcacggcg
     4681 tgatggtgga tttgccgaat atattgtcgt taagcggaaa aatgtctttg ctctacctac
     4741 tgatatgcct attgaggatg gggcttttat tgaaccgatt accgttggct tgcatgcttt
     4801 tcatttagcg caaggttgtg agaataaaaa cgttattatt attggtgccg gaaccattgg
     4861 cctgctggct attcagtgcg ctgtcgcgct gggagcaaag agtgtgacgg ctatcgacat
     4921 tagctcagaa aaactggcac tggcaaaatc tttcggtgcg atgcaaacat ttaacagtag
     4981 cgaaatgagc gcgccgcaaa tgcagagcgt tttacgcgaa ctgcgtttta atcagcttat
     5041 cctcgagacg gctggcgtac cgcaaactgt cgaactggcg gtagagattg ccggtcctca
     5101 tgcccaactg gcgctggtgg gcacgttgca tcaggatctg catttaacat cggcaacgtt
     5161 tggcaaaata ttgcgtaaag agctgacggt tatcggcagt tggatgaact attccagccc
     5221 ttggccgggg caggagtggg aaacggcgag ccggttgctg acagaacgta agttaagcct
     5281 ggagccatta atcgctcacc gtggaagctt tgaaagcttc acccaggcgg tgcgtgacat
     5341 cgctcgtaat gccatgccgg gcaaagtgtt gctcattcca tgaaaccgcg ggccagcgtg
     5401 atgctggccc ggtattgtgc aaaacagatc attcaccaat ggtccccctt cgtttacact
     5461 agccacaatt gaatatgggt aaatgacgat gaattcattc gagcgaagga ataagatcat
     5521 ccaattagtg aatgaacagg gaaccgtgct tgttcaggat ctggcgggag tatttgctgc
     5581 ctcggaagcg acaatccgtg ccgatttgcg ctttctcgaa caaaaaggcg tggttacgcg
     5641 ctttcatggc ggtgcggcga aaataatgtc cggtaatagt gaaaccgaga cccaggaagt
     5701 cgggtttaaa gagcgatttc agctcgccag cgcgcccaaa aacagaatag cgcaggcggc
     5761 agtcaaaatg atccatgaag ggatgaccgt tattctcgac agcggaagta ccacaatgct
     5821 tatcgccgaa ggattaatga ccgctaaaaa tatcacggtg attaccaaca gtctcccggc
     5881 tgcgtttgcc ctttccgaaa ataaagacat tactctggtc gtctgcggtg gcacggtccg
     5941 ccataaaacg cgatctatgc atggatctat tgctgaacgt tccctgcagg atattaatgc
     6001 cgatttaatg tttgtcggtg ctgatggtat tgatgcggtt aatggtatta cgacctttaa
     6061 tgaaggttat tcgattagcg gtgcgatggt caccgccgct aataaagtga ttgccgttct
     6121 cgattcatcg aaatttaacc gtcgcggttt taatcaggta ctgccaatcg aaaaaattga
     6181 cattattatt accgatgatg ctgtatcaga ggtggataaa ctggcattgc agaagacacg
     6241 ggtaaaatta attacggtgt agtattgttc tgtatgccgg ataaggtatt tacaccgcat
     6301 ccggcatttc ggattctccc tggttcaaga gagaatagtt tgattaacgc aataatggac
     6361 aatctggtgg taatcgacaa cgcaacgccg ccggaagtat ttcaatatga aagttttgtc
     6421 cgctcaacgg ttcgccatca agattaaaag tgatttcgtg tggggcttgt atatcaaacc
     6481 acgacgaagc gccttcgata atattcgggt tatcttcgtc agattttaag gttgatacga
     6541 gagccggaag aatttcatcg ccggtaaaaa tgcgcagttg cagcaagcca tcgttaatta
     6601 acgcgttcgg acacaattgc tgaccgccac cggcctgacg cccgttacca ataccaatga
     6661 ccagggcgtc accttgccag tgaaagtttt caccgcggat ttcacaacgg tccggttgca
     6721 gagtatccat gcgcattaag ccatgaatga tgtaagagac gccacccagc gcggctttta
     6781 atttttccgg cgtttctgtg gtaatacgcg tcccaaatcc gcctgtcgcc atattaataa
     6841 aacaggtttg tttgttgacc tgcgccatat ctatcgcaat ggcgttaccg gcaattgcca
     6901 gtttcagcgc cttatccagt gcctcaggaa tccctacact ggtggcaaaa tcattggcgg
     6961 ttcctaatgg caaaattccc agcgcgggta tgtcatcccc ctcacactga atcaacgccg
     7021 tagaaacttc attaatggtg ccatcgccac caccggcaat caccgttgcg acgcccaact
     7081 tccgggcctc ctctacatat cgtgcggcat cgcctttctc ccaggtgacc cgcacatgga
     7141 tcgtcattcc ttcctcacgc aacagcataa ttgcttcgcg caagggtaga ttgtcagtac
     7201 ttttgccatt aagaatcagt aagctggcgg gaaattctgc catgatcgta tgtgccttta
     7261 tgattggtct gtagatagtg tagagcagaa aacaaaaggt ggagtcagaa caggatgaaa
     7321 gtcggaggat aaaaagtcag ttcaagtatg gaggattcag tggttaccac caggagcttg
     7381 caatggggac agcgaatact aacggctgcc accgataaat ttcaaaaaag agcatatacc
     7441 taatattcaa ctaaacagtg gcatcttcaa tataatatat taaagccccc atggagttac
     7501 cctgaagggc ctcaatgtcc gtaattccta cttatgtagg aaat
//
LOCUS       DAPGWD010000193         7471 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPGWD010000193 DAPGWD010000000
VERSION     DAPGWD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.194"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..191
                     /locus_tag="RHX88_003999"
     CDS             <1..191
                     /locus_tag="RHX88_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2232626.1"
                     /translation="AFSTNADDKIILKHISVSSVSASPTVLEDTIADITRKYNASSWK
                     VTSMRIDNNSTATAVLYK"
     gene            203..796
                     /gene="rclC"
                     /locus_tag="RHX88_004000"
     CDS             203..796
                     /gene="rclC"
                     /locus_tag="RHX88_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDV2232627.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVIIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(956..1825)
                     /locus_tag="RHX88_004001"
     CDS             complement(956..1825)
                     /locus_tag="RHX88_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV2232628.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            2074..2931
                     /locus_tag="RHX88_004002"
     CDS             2074..2931
                     /locus_tag="RHX88_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2232629.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(3052..7305)
                     /gene="fdeC"
                     /locus_tag="RHX88_004003"
     CDS             complement(3052..7305)
                     /gene="fdeC"
                     /locus_tag="RHX88_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDV2232630.1"
                     /translation="MSRYKTGHKQPLFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     AHAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVIPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTKLTFTV
                     KDAYGNLVTGLKPDAPQFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQASLEVDSAAPKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSDTQTITFVADKATAIVSGIE
                     VIGNYALADGKAKQTYKVTVTDANNNLVKDSDVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         1865 a         1919 c         1661 g         2026 t
ORIGIN      
        1 tggcattttc caccaatgca gatgataaaa taattctgaa acatatcagc gtctcgtcag
       61 tatcagcatc accgacagtt ctggaggata ccattgctga tataaccaga aaatataatg
      121 cttcatcctg gaaagtcaca tcgatgcgaa ttgataataa ttcaaccgca acagcagtat
      181 tgtataaata aggatgttca caatggaaaa atacctgcac ctgttaagtc ggggagataa
      241 aattggcctg acattgattc gtctgagtat tgcaattgtt tttatgtgga ttgggttatt
      301 aaagtttgtc ccttacgagg cagacagcat tacaccattc gtcgcaaaca gtccactaat
      361 gtcgttcttt tatgaacacc cggaagacta taaacagtat ctgactcacg aaggcgaata
      421 caaaccagaa gcaagggcat ggcaaacggc caataatacc tatggttttt ccaacggtct
      481 tggcgtcgtg gaggtgatta ttgctctgct ggttttggct aatcctgtca atcgctggtt
      541 aggtttattg ggaggactga tggcatttac cacgccgctg gtaacactct catttttaat
      601 caccaccccg gaggcatggg tacccgcatt gggtgacgct catcatggtt tcccttattt
      661 atccggtgct ggtcgcctgg tattgaaaga tactctgatg ctggcaggtg ctgtcataat
      721 aatggcagat tcggcgcggg aaattcttaa acaacgcagt aatgaatcca gttcaacgtt
      781 aaaaactgaa tattgataat ccgaacactg tctgttgttc actttttcag gcgtcaggcc
      841 ccctttctga tgatgaagcc tgacgccata ttaccggaga tatgacagag tgaagatcaa
      901 tatcactccc ctgttaaaag attcttgtcg tctcaggtaa aatatatgat taatatcaat
      961 catgtatttt tacagcaaga caaccacgaa caaattcagg attaaaatgt tttaccgact
     1021 ccccaacata acccaaatca agcgaactaa tttgtgccat ttctttctct gtcagtgaga
     1081 aatcccagat agcaaaattt tcttcgatac gctgttgatg tgtcgatttc ggaataacga
     1141 ccactccccg ttgaatattc caacgcagaa tgacttgaga aatcgatttt tgatgggcat
     1201 ctgcaatact ctgaagcaga ttattttcaa agggtttatg tcgtccacca cctaacggag
     1261 cccatgcttc aggctgcacg ttataatact tcatggttgc cagcgcctct ggttgggcaa
     1321 aatagggatg caactcgacc tggttgacca tcggtttaac tcttaccgtt tcacaaaagt
     1381 tcgccagaac atgaggatag aaattggaaa ccccaattgc ttttaatttg ccctcttcat
     1441 aggcatcttc gagtgcacgc cacgcactaa aataatcgcg catagcctgg tgcaataaat
     1501 agaggtcgaa gtactctagt cccgattttt ttaatgatgc ttcaatacct gctgctgccg
     1561 tatcctgatt caacatatcc tgcacccaca atttagacgt tataaataac tcatctcggg
     1621 tacataaacc ttcagatata gcttcacgaa cggcttcacc gacggcatct tcattaccgt
     1681 ataccgctgc agtgtcgatg agtcgataac ctgtgcggat agcgttcaac actgactgtt
     1741 tgcacacgtt tttatcggta acctgaaaaa caccaaatcc catcatcggc attttcagat
     1801 tgttacttaa tacggaaaat tccactgtca gtccctccat taggcatgaa caatgagtct
     1861 acgttaaaac gtaacctcaa attagtacgt ggattttgat atcacttatg caaaaaaatc
     1921 attaataatg caggactgaa acctctctat tttcggggac aacgaagcag acgctaccag
     1981 tgcttttgcc ttcgcccttg ctgtttttga tacacttagg gcccagggca taacgaaaat
     2041 gtgcgatatg acaaagaatt aacggagaat gtgatgatca ggcagaagat tctacagcag
     2101 ctcctggagt ggattgagtg caatcttgag caccctattt caatcgaaga tatcgcacag
     2161 aaatctggct acagcagacg caacatccag cttctgttcc gaaatttcat gcatgtgcct
     2221 ttgggagaat acattcgcaa acgaaggctt tgccgtgccg ctattcttgt ccggctcacc
     2281 gcgaaatcta tgcttgatat tgcactctct ttgcattttg attcacagca gtcattcagc
     2341 cgcgaattta aaaagttatt cggctgctct ccccgtgaat accgccaccg tgattattgg
     2401 gatctcgcaa atatcttccc ttctttttta atacgtcaac agcaaaagac ggagtgtaga
     2461 ttagtcaatt ttcctgagac acctattttt ggcaactcat ttaaatatga cattgaagtg
     2521 tcgaataaat caccggatga agaagtcaaa ctgcgacgtc atcatttagc cagatgtatg
     2581 aataatttta agacggatat ctatttcgtc tccacgtttg aaccgtcaac aaaatcggtc
     2641 gatttgctca cggttgaaac ttttgccggt acggtatgca aacaaaccga ttcagagatg
     2701 ccgaaagagt ggacaattaa ccgaggactg tatgcctctt tccgctatga aggtgagtgg
     2761 gagcactacc ctgaatgggc gcgaaacctc tatctgatgg aattacccgc cagggggtta
     2821 gccagagtga acggcagtga tattgagcgg ttttattaca atgaagattt cgtagaaaag
     2881 gatagcaatg atgttgtttg cgaaattttt atccccgttc gtccggttta gttttcgcta
     2941 tatctaaatg aatttataac gagactactg tcaggaaatt caataaataa tggcgttgaa
     3001 gatgtcaacg ccattattgt ttattagaat gttactttca tattcttaaa attatttcat
     3061 cgcctcctcg tcctttgccg tatcggcatc caccgcggta atggtcaccg tcactggctt
     3121 catttcgtac ccgccatagg tcagggcgtt aaatgtgaac gtatagacac caggcttgct
     3181 ggtactaaat ttacgcgtga tttttccgct cacgaaatgg tcatttttcg acggcaggaa
     3241 ctgataatcc ttctccgtca ccccttccgg cgtctgaatg tcgacccaca tattcccgcc
     3301 aacggggttg tttgccgaca tcagtgtcac ctccagcttc gcagtcgatt tcccatccgc
     3361 caccagagat tgcatatcag atgatgcggt gagtaccgcg tttttatcat ccgcaacgaa
     3421 tttagattcg gcagttttcg ttgatacctg accgttggtc tgactcactt gcgccttgag
     3481 tgtgtatgtc gccgcaacag tagtggtagc ggtgaaaata gcctgccctt gctcattcgt
     3541 tgtcgcagtg ccattcggtt ccaggtttaa cgaagccggg ctggcagtca gcgtcacgtc
     3601 gctatctttc accaaattgt tattggcatc agtcaccgta actttatacg tttgtttggc
     3661 tttgccgtcc gccagcgcat agttgccaat cacctcaata ccggagacga ttgcggtagc
     3721 cttatcggct acaaacgtta tagtctgcgt atccgaaggc tggctattcc ccgccgatgc
     3781 cgtgatctca tacgttccgg cagtcactga aaccacctgc aactctgctt tcccctcatc
     3841 gttggctttc acccagacat tatcgcctgt aagcggcttg acaccccggg gcagattaaa
     3901 ggtcaccagg ctccctggaa cagggttacc atttttatcc tcaacggtcg ccgtcagcgt
     3961 aaaggcatct ttgccgtttg ctacttttgg cgcggcgcta tcaacctcca gactcgcctg
     4021 accggtactg aaatccgcct tgaatttcac cgtaaccgtc ttctgagcat tattcactga
     4081 ggccgtgatg ctgtgttccc cggcaaccgt tgacatcagc tcaatgtcgg ctttacctgc
     4141 cgcatcggtt gttaccgtat tccccgtctt gctggtcaca cctttcggca gagtcagcgt
     4201 cacattttgt ccctgcaacg ggttaccata ggtgtccaca acggtcaggg ttacctggtt
     4261 agtcgattgt ccattagcca gctggttatc aaccttcacc gtcatatcac gaatctcagc
     4321 cttagatgcg tcaccagcaa cattcagcac cagtggctga gcaacggcat tttggccgtt
     4381 cactcgcggc atcacagaca actggcccgc ggcagatccc agagtcaagg tcgccacgta
     4441 gaccccatta cttgtttctg tccagtctcc tgttgaaggt cgctctgtcc ccgtgctggc
     4501 ggcaccacta aactgcggtg catctggctt cagcccggtg acaaggttcc cgtacgcatc
     4561 cttcacggtg aaggtgagtt tcgtcgtcgt tttgacggtc ggagccttat tgtcagcaac
     4621 aagcgtagag ttcgctgatg acatctcccc cgcaatgaca gtcaactgcg cggcttcggt
     4681 ggctgctggc tggccgttga atagcggcat gacgagaagc tcacccgtct ttccacctgt
     4741 tgtcagcgta gcgacatagg aaccatcacc tttttcggtc caaccagaaa cagtcgcccc
     4801 ttcagacgca gcacccgtca ggcttgccga cagggaaaga ccactgatag cgttgccatg
     4861 tgcatctttt gcaataagcg tcacggttgt gctttcgccg gcttttacgt gatcttctgc
     4921 gacagagact ttcgactggt ttgaagataa cgcatcagcc accacggtta cttttgccgc
     4981 atttgctgcc gcatcctgtc catttagctt cggaataacc tctaattcac ccgccgtagt
     5041 gccgagagaa atctgcgcag tccaggtccc gtcgccatta tccgtccagc ctgatgccgt
     5101 agaaccagca gcagctgcgc ccgataatga cggtgcatcc ggattgagac cagttacagg
     5161 gttgtcatta gcatcttttg ccgtccagat tgccgtaact gtaccgccaa ccaccggttt
     5221 atcaggattc agtgtgatgg acgaatgtgc tgcatcaagc ggcccggcaa caaacttcag
     5281 cgtttgttgt aatgtgccca gctgatactg ctcgctgaaa gcacgcacaa caacgtttcc
     5341 ggcacgggtc gaagaaaccg tggcgctgta aacgcctggt tttatttccg aaatggcacc
     5401 aacggttaca ccattagtgt cttgcggaac aaatcgcaag cggctggctt ctcccgtcac
     5461 cggattaccc tcggagtcca ccgcagtcaa cgtcaacgta taggcttgct gaccatcagc
     5521 aaccacgtca cctgacggct cgttagcgct cagggtggag tttgccacat ccatcatcgt
     5581 ggcccgcagt tctgcagtga cggttttgct catgccatca acactaacag taatcgttgc
     5641 ctcacctgac tgcgttccag tagtaaagac agactgatac acccctgctt cagtttcggt
     5701 gaactcaccc agtgttggct ttgcctgtga tttagtggcc ttcagggtac gagtcacaat
     5761 atttccagcc ggtttgaaag ttagttcagt cttgatctga tctttcatgc ccgtgactgg
     5821 ctggccctcg gcgtcgcgca gagacagcac cagcggtctt tgctcattac cgttagcaag
     5881 catttgaata cggctctgac cgtcaagcgt taacgccgtg cgatcggcgc tcataccagc
     5941 tccggtaatg accacctctg tctgcacgcg ttttgaggta ttgcctttgt tatcgtaggc
     6001 aaccgcagaa atcgcataat aattgtcttt gcctggacga taagccggga gcgttacttg
     6061 ccactgacta ccctgaccgg taattttgcc accttcagcc agtaatgacg gcgcttccca
     6121 ctgcacattt ttcagtccgt gagttgcttt gctgaccaca agcccgaggg aaagtgtctg
     6181 accgcccttg ccttcaatac gctcaggcag agcaatacgg atcacttcag atttgcggta
     6241 ctcaagaacg atgttgttat tacgctcaac caggtcatag cggctgcctg ccagcatgcg
     6301 acgctcgcga atgctgtctg tatcgagttg tttctccaga ggttcgccaa tccggtagtt
     6361 aacttccagg ccaaagcgag tgtcattctc accgctcttg ccctgcttat gcccggcgct
     6421 cagtgtcaga agaggcactg gcgtataggt cacctcggca gaaatagcat gcgggtcttt
     6481 ctggcgctta tctttaccaa acagcccgac ttcatcgcca taatactgtt catacatcag
     6541 gcttgcgcca agctgcggcc aggcaggtaa atagccctct gcgcggatat cccaaccatt
     6601 cgccgggcgt tcctgataat cctcaatatc cggcgatttt ttccagccag aagcccgaat
     6661 ataaccattg gcgctcagtt tcagataatc gcgccagtat tccgcaccaa caccaatgcg
     6721 ggtatgacta cgggataaat catgatcgat aaaagtattc acccccgcca tccagtcatt
     6781 tcctgaaaaa tgacgccagc caaaaccaat atttgactga gtacgatcgt ctgtacgatg
     6841 tattgccccc tgagtgaaca acatatttgt cggcgtatca taaatcggat aaagcatttc
     6901 cagcgaagag tccttcagcg agaaatcttt atcgacattc agtttgacgc gcgcagtacc
     6961 gtatttcccg agccactcct gtatttcctg gttagcttta gcggtggcca ttccggtaat
     7021 aaaattacgt gtcgcatcgc tatctggctg actgcttaaa aatgtcccgg catttgcggc
     7081 aaacgacgcg acatttttct ccacattatt atcagcagtt accgtggtat tttccatgct
     7141 caaccgtggc tgaaccgcat gctgcgcatg tgccgccatt actggggtaa aggtgacagc
     7201 gagtggaaaa agaacctgaa cagagatatt tgcccacgcc acgcagcggg ccagaactga
     7261 ataacgaaat agtggttgtt tatgacctgt tttataacgt gacattgaga taatttcctg
     7321 atgaacgaat taacgaacat aggcagacac aaataatggc gtctgcttgt cagaaaaacc
     7381 ggggatttcc cggtttcctt ttccgcagaa aaggaaatac ctttatgtaa gaaagtacac
     7441 cgccacctaa tatatccagt gcctgtagcc a
//
LOCUS       DAPGWD010000194         7299 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPGWD010000194 DAPGWD010000000
VERSION     DAPGWD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.195"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..474)
                     /gene="folK"
                     /locus_tag="RHX88_004004"
     CDS             complement(<1..474)
                     /gene="folK"
                     /locus_tag="RHX88_004004"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDV2232631.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSK"
     gene            complement(471..1889)
                     /gene="pcnB"
                     /locus_tag="RHX88_004005"
     CDS             complement(471..1889)
                     /gene="pcnB"
                     /locus_tag="RHX88_004005"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDV2232632.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1928..2854)
                     /gene="gluQRS"
                     /locus_tag="RHX88_004006"
     CDS             complement(1928..2854)
                     /gene="gluQRS"
                     /locus_tag="RHX88_004006"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDV2232633.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2891..3346)
                     /gene="dksA"
                     /locus_tag="RHX88_004007"
     CDS             complement(2891..3346)
                     /gene="dksA"
                     /locus_tag="RHX88_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDV2232634.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3524..4228)
                     /gene="sfsA"
                     /locus_tag="RHX88_004008"
     CDS             complement(3524..4228)
                     /gene="sfsA"
                     /locus_tag="RHX88_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDV2232635.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4243..4773)
                     /gene="thpR"
                     /locus_tag="RHX88_004009"
     CDS             complement(4243..4773)
                     /gene="thpR"
                     /locus_tag="RHX88_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDV2232636.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4847..7276
                     /gene="hrpB"
                     /locus_tag="RHX88_004010"
     CDS             4847..7276
                     /gene="hrpB"
                     /locus_tag="RHX88_004010"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDV2232637.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSML
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDNESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1650 a         1905 c         2047 g         1697 t
ORIGIN      
        1 tttgcttaat ttgtcaaatg ctcttgtatg taagacttca cgcaacgtct ccccatcagg
       61 aaacgccagc tccggtgcaa tctcaaacag cggccacagc ataaatccac gattcttcat
      121 atcgtagtgc ggaacggtca ggcgttcggt atttatcact tcattaccaa acagcatgat
      181 atcgagatcc agcgtgcgtg gtccccagcg ttcagcttta cgaacacgac cctgttgcag
      241 ttctatgcgc tgtgtatggt tgagtaactc ttccggggcg agcgaagttt caagcgccac
      301 ggcggcgttg aggtaatcgg gttgatcttg cggccccagc ggtggggtgc ggtaaaacga
      361 agaaacggca agaatgtggc tttcagggat atcgcctaat gctttcaggg cagcattgac
      421 ctgctccagc ggagaggcca gattgctgcc tatggcaata tacgccactg tcatgcggta
      481 ccctcacgac gcggtgcgcg tttgcgtgga cgacgagtac gacgacgcgg tgacggttct
      541 tcatccagct cgttaagcat ccctttttga tctggtggtg cggaaacctg gaactcaccc
      601 caccatttca ccagacgctg cagttcagca ttacgttcaa cttcagctcg caaggccaac
      661 aggtcataag ccgcacggaa cttaggatgc tccagcagtt tccatgcgcg tttaccctga
      721 cgacgggaca tacgcaactg caactgccag atatcgcggg ttaacgtcgt cagacgtttc
      781 gggattgcca gtgaacggca ggcttcgtcc agcacgtcgt tcatcgccag cgcgaaagcg
      841 tcgtgatagg tcaggccgct ttcctgggcg atcttctgtg ccgtctccag cagtgggtac
      901 cagaacatgg cggcaaacaa gaacgccggg ttcacgcgca tatcgttatg gatacgcgta
      961 tcggtattct tcagcacctg ttcaatgatc cgctccatcg ggctgtcgcc attttccgtg
     1021 aagtagcggg taatggtcgg gaacagcggc tggaacagat gatattcaca caacagctta
     1081 taggtttcgt aaccgtagcc cgcttgtagc agtttaagcg attcttcaaa caggcgtgcc
     1141 ggtgggatat cgttcagcag ggtagcgagg cgagggatcg gttctgcggt ttccgggctg
     1201 atgcgcatac ccaatttggc ggcaaaacgt accgcgcgca gcatacgtac cggatcttca
     1261 cggtagcgcg tttccgggtt accaatcaga cggataacgc cgtccttcag atccttcata
     1321 ccgccaacgt aatcacggac ggtaaaatcc gctacgctgt aatacaggct gttgatagtg
     1381 aaatcgcggc gctgggcgtc ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacatg
     1441 ccgttttgcc cgcgttggga ggtcgtgcgg tcgctgacgt taccttcgtg gtgtccacgg
     1501 aaggttgcaa cttcgataat ctccgggcca aacatcacat gagccagacg gaaacggcga
     1561 cccaccaggc ggcagttacg gaacagtttg cgcacctgct caggcgtggc gttagtggtt
     1621 acgtcaaaat ctttcggctt tttgccaagt aacaggtcgc gcacgccgcc gccaaccagc
     1681 caggcttcgt atcccgcttt gttgagcctg tacattacct tcagggcatt ttcactgata
     1741 tctttgcggg aaatagcatg ctgctcacgc gggatcaccg tcacctgtgg acgggcgact
     1801 gcctgttcag cctcgctttc ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg
     1861 gtaaaaatag tacacctcgg tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa
     1921 tcatagctca gcatgacgca tttgagaatg ttgaatttac aattgccgac tcgggcacgg
     1981 cggtaagccg ccagtttttg acggctgact gaaggatttg ctcgacgctg aaatcctgcc
     2041 agtgtgcttc tgcctgctgc cccagaaatt gaagtgccgc gattagtacc gggcgtggat
     2101 cgcctttcgg caacgcaggc gcatgattct gcttggaaag tttagcgcct tgtggattaa
     2161 gcgccagcgg cagatgaatg taatctggca ctttccagcc aaaaagctgg tacagcgaga
     2221 tttgccttac tgttggttca atcagatcag ccccacgcac tatttctgta acgccctgga
     2281 aatgatcatc aaccacaaca gccaggttgt aggcgaacaa cccatcacgg cgatgaatga
     2341 taaaatcttc ccgtgccagt ttttcgtcgg cgtgaataat gccgcgcagc tggtcagtaa
     2401 attgcgtgac cggatgctgc tggcggatac gcactgcggc attgtctggt ccatgatgca
     2461 acacccggca atgaccgtcg taaataccgc caatgctttg aatacgcgca cgcgtacagg
     2521 tgcagtaata acttagtcct tgttcatgta accaggcgag tgcttcacga taggcgtggt
     2581 gacgttgcga ttgccagaga acatcgccgt cccagtgcag accgtaatgt tccagctggc
     2641 gcaggatagt ttctgcggca ccgggaactt cacgaggcgg gtcgatatct tctatgcgta
     2701 ccagccagcg accttgccgg gcgcgagcct gcaaatagct gccgagcgcg gcgatcagag
     2761 agccaaaatg aagctcgccg gaaggagagg gggcgaagcg gccaatatac tgtgtgtctg
     2821 tcatctcttt gaacaaaaaa taaggcggga gcatttcccg cctgtggtaa acgtgatgga
     2881 acggctgtaa ttagccagcc atctgttttt cgcgaatttc agccagcgtt ttgcagtcga
     2941 tgcacagatc ggctgtcggg cgcgcttcca gacggcgaat accaatttca acaccgcagg
     3001 attcgcagta gccgaaatct tcgtcttcca cttttttcag cgtcttctcg atctttttga
     3061 tcagcttacg ctcgcgatcg cggttacgca gttcgaggct gaactcttct tcctgggctg
     3121 cacggtctac cgggtccggg aagttggctg cttcatcctg catatgtgta acggtgcgat
     3181 cgacttcatc cctgagttga ttacgccatg cttccagaat acgacggaag tgcgccagct
     3241 gggcttcatt catatactct tcgcccggct tctcctgata tggttccacc ccagcgatgg
     3301 cgagaatact cagggacgat gttttacggt tttgcccttc ttgcatgttg cttctcctta
     3361 acacgcacta tcgatcccca tgttcggggg aaaaatgagg ccgctataaa tagcagatgc
     3421 ttttccggat agcaattatc taaacgtaac acttgacaac tgtgtgagga aaagcgtatt
     3481 tgcgcacgcg accagaatgt aaattaacca gttacttact ttactacaat gtaaccggca
     3541 gtgatttttt aagagccatg ccttcagcag aaatttccgc tttgtaagcc agaatttcta
     3601 cccccctctg ttgagcttct gacaatagtt gcgcgtattt ctcatcgatg tggcgcgcgg
     3661 gtgaaaaccg tgtaatggct gaatgcagca cggcgaaaaa gataaccgca cgctggcctt
     3721 cagccgctac gctcatcaac tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg
     3781 gaaaatatcc ctgttcgttc tccgctaacg taaccgattt cacttcaata tagcagtctg
     3841 gacgcgaatc cgcctgcaac ataaagtcaa tacggctgcg ttctgcgccg tattttactt
     3901 cgcttttcag cgagctatag cctgacagtt ctgaaattga ttcattaagg atagcctctt
     3961 tcgtcaacct gttagcccaa agcgtgttga cgcaaataaa tgcgccgctc tggctttgag
     4021 ttaattccca ggtgtgtggg tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg
     4081 tatcgccagg cgttgcacaa ccggtcattg cacccgtatt cgggcagtgt agcgttaatt
     4141 cgcgaccatc gggtgtgatc acatcggcta aaaaacgttt gtaacgctga attagcgtcg
     4201 cgcgctgtag agggggagag aattccattg cgacaatcct tgttattgcg ttagcgccca
     4261 gcgttttagc ggcgtgtagc gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa
     4321 ctccgtcacc gcataagacc agttaaaacc tggcggcggg attgtcaccg cctcgctggc
     4381 gtcgcgcaat aaggtaatat gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg
     4441 ggcagcctgt gaacggagca tattcgccag ctggattaag ccgcgtggag gctgacgcat
     4501 ccctaaccac accacacgcg aacgcagcca ttgtccggcg tcatcaagcg tgagtgtgaa
     4561 accaggttga cgaatccgtc cggctaaaag agaaagcgcc ttctctttct cggcgctcac
     4621 ttcgcctaaa aatgccagag tcagatgcaa attatcggcg gcgaccggac gtcccgcctc
     4681 aggtgggaag tgtgtggcgc gccaatggat aatctgttcg cggatttctg caggtaagtc
     4741 gatagcaaag aacagacgtt gcggttcaga catgtgaggc actcggttat gaattaccgc
     4801 gatgctacaa tgtggcgcga agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc
     4861 cgttgctgcc gtcttatctg aattacttac cgctctcgat tgtgcgccgc aggtattatt
     4921 aagtgcgccg accggggccg ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc
     4981 cggcattaac gggaaaatta tcctgctgga gccgcgtcgt ctggcggcgc gtaacgtcgc
     5041 gcaacggctg gcggagctgc ttaacgaaaa gccaggcgac accgttggct accggatgcg
     5101 tgcgcaaaac tgcgtcgggc cgaatacccg cctggaagtg gttaccgaag gcgtgctgac
     5161 gcgcatgatc cagcgtgacc cggaactgag cggtgttgga ctggtgatcc ttgatgaatt
     5221 tcatgagcgc agcttgcagg cggatttggc gttggcgctg ttactcgatg tgcaacaagg
     5281 tctgcgtgat gaccttaaac tgctgattat gtcggctacc ctggacaacg accgcttgca
     5341 gcaaatgctg ccagaagcgc ctgtcgtcat ctcagaaggg cgctcgtttc cggttgaacg
     5401 ccgttattta ccgctgcccg cgcatcagcg ttttgacgat gccgttgcgg tagccaccgc
     5461 tgaaatgctg cgtcaggaaa gcggatcatt actgttattt ttacctggcg tcggagaaat
     5521 tcagcgtgtg caggaacaac tggcttcgcg catcggcagt gatgtattgc tctgcccgct
     5581 gtatggcgcg ttgtcgctga acgatcagcg aaaagcgatc ctcccggcac cgcaagggat
     5641 gcgcaaagtg gtgctggcga ccaatattgc tgaaaccagt ttaaccattg aaggtattcg
     5701 tctggtggtg gattgtgccc aggagcgtgt ggcgcgtttt gatccgcgca cggggcttac
     5761 gcgactgatt actcaacgcg ttagccaggc atccatgacg cagcgtgccg ggcgcgccgg
     5821 gcgtctggag ccgggtatct gcctgcattt aatcgccaaa gaacaagcag aacgcgccgc
     5881 ggcgcaaagt gaaccggaga tcttacaaag cgatctttcc ggtttgctga tggaattact
     5941 gcaatgggga tgcagcgatc cggcgcagat gagctggctg gatcaaccgc cagtagtgaa
     6001 tctactggcc gcgaaacgtc tgttacaaat gctgggggca ctggagggtg aacggcttag
     6061 tgcgcaaggg caaaaaatgg cagcgctggg taacgatccg cgtttagcgg caatgctggt
     6121 tagcgcgaag aacgacgacg aagctgctac cgcggcaaaa attgccgcca ttctcgaaga
     6181 gccgccacgg atgggcaata gtgacctggg cgtggcgttt tcgcgcaatc aaccagcctg
     6241 gcagcaacgt agtcagcaac tgttaaaacg cttaaacgta cgtggcggtg aggcagacag
     6301 ttcgcttatc gcgccgctac ttgccggggc gtttgccgat cgcattgctc gtcgccgtgg
     6361 gcaagatgga cgctatcaac tggcaaacgg catgggagcg atgctcgatg ccaacgacgc
     6421 gctaagccgc cacgaatggt tgatcgcacc gttattattg cagggcagcg cctcgccgga
     6481 tgcgcggatt ttactggcgc taccggtcga tattgatgaa ttagtacaac gctgcccgca
     6541 gctggtacag cagtctgaca ctgtggagtg ggatgacgcg caaggtacgc tgaaagcctg
     6601 gcgtcggctg caaatcggtc tgctgacggt gaaagtgcag ccgctggcga aaccgtcaga
     6661 agacgagttg catcaggcga tgcttaatgg cattcgtgat aaaggtttaa gcatgctcaa
     6721 ctggacggcg gaagcggaac agctacgctt gcgtttgtta tgcgccgcaa agtggttgcc
     6781 ggaatatgac tggcctgcgg ttgataatga aagtttgttg gcgacgctgg aaacgtggct
     6841 gctgccacat atgaccggcg tgcattcact acgcggcctg aaatcactcg acatttatca
     6901 ggcactacgc ggattacttg attggggaat gcagcaacgt ctggatagtg aattgcctgc
     6961 gcattacact gtgccgacgg gaagccggat cgccattcgt tatcatgaag ataacccgcc
     7021 cgcgctggcg gtgagaatgc aggagatgtt tggtgaggcc accaatccga cgatcgccca
     7081 ggggcgcgtg ccgctggtgc tggagttgct ttcacctgcc caaaggccat tacaaatcac
     7141 acgagatttg agcgccttct ggaaaggagc gtaccgtgag gtgcaaaaag agatgaaagg
     7201 gcgttatccc aaacatgtct ggccggacga cccggcaaat actgcaccga cgcgacggac
     7261 gaaaaagtat tcgtaaggtt ggttttctcc ctctccctg
//
LOCUS       DAPGWD010000195         7093 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPGWD010000195 DAPGWD010000000
VERSION     DAPGWD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.196"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            134..1317
                     /locus_tag="RHX88_004011"
                     /pseudo
     CDS             134..1317
                     /locus_tag="RHX88_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
     gene            1428..2351
                     /locus_tag="RHX88_004012"
     CDS             1428..2351
                     /locus_tag="RHX88_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2232638.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(2355..3173)
                     /gene="nlpA"
                     /locus_tag="RHX88_004013"
     CDS             complement(2355..3173)
                     /gene="nlpA"
                     /locus_tag="RHX88_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDV2232639.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            3395..3688
                     /locus_tag="RHX88_004014"
     CDS             3395..3688
                     /locus_tag="RHX88_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232640.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(3729..4919)
                     /gene="nepI"
                     /locus_tag="RHX88_004015"
     CDS             complement(3729..4919)
                     /gene="nepI"
                     /locus_tag="RHX88_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDV2232641.1"
                     /translation="MSEFIAENRGADTITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(5130..5582)
                     /locus_tag="RHX88_004016"
     CDS             complement(5130..5582)
                     /locus_tag="RHX88_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDV2232642.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5635..6969)
                     /gene="adeQ"
                     /locus_tag="RHX88_004017"
                     /gene_synonym="yicO"
     CDS             complement(5635..6969)
                     /gene="adeQ"
                     /locus_tag="RHX88_004017"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDV2232643.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
BASE COUNT         1915 a         1785 c         1613 g         1780 t
ORIGIN      
        1 ctggccctat aatattgcgc tagcattgct ttctggttgt atcagcgata ctcaaaaatt
       61 cttaatacaa tactcattcg actggtactt atttgtaact cagttatatt ttttcgcccg
      121 gtgattcaga agaatgcaaa aaacggctac cactccatca aaaatacttg atctcactgc
      181 cgcggcattt ttacttgtcg cctttctgac gggtattgcg ggcgctcttc agactcctac
      241 cctaagtata ttcctcgcag atgaactgaa agcccgtcct ataatggtag gtttttcttc
      301 accggtagcg ctattatggg aattctggtc agtcaatttc tggcaaggca ctccgataaa
      361 caaggcgacc gtaaattact gattctgcta tgttgcttat ttggagtgct ggcctgcacg
      421 ctttttgcgt ggaatcgcaa ctacttcatt ctcctctcaa cgggcgtact tctgagtagt
      481 tttgcttcca ccgcaaaccc gcaaatgttc gccctcgccc gtgaacacgc cgacagaaca
      541 ggccgtgaga cggtcatgtt cagtacattt ttacgtgctc agatctcgct tgcctgggtt
      601 atcgggccac cgctcgctta tgaactggca atgggattta gttttaaagt gatgtatctc
      661 accgcagcca tcgcatttgt tgtttgcgga ctgatagtct ggttgttttt gccatcaata
      721 caaagaaata ttcctgtcgt tacccaaccc gtagaaattt taccctccac ccacaggaag
      781 cgggatacgc ggctactttt tgtggtctgt tcaatgatgt gggcggcgaa taatctctac
      841 atgataaata tgccgctatt tattattgat gaactgcatc taaccgataa actggctgga
      901 gaaatgattg gtatcgctgc cggtctggaa attccgatga tgttaatcgc aggctattac
      961 atgaaacgta ttggcaagcg actattaatg ctcattgcta tcgtgagtgg aatgtgtttt
     1021 tacgccagcg tactcatggc gacgactccg gcggttgagc tggaattgca aattcttaat
     1081 gccatcttcc ttggtattct ctgtggtatc ggcatgcttt attttcagga cttgatgcct
     1141 gaaaaaatag gctctgcgac aacgttatat gcaaatactt cacgcgtcgg ctggattatc
     1201 gccggctctg ttgacggaat tatggttgaa atctggagct accatgcgtt gttctggctg
     1261 gcgataggga tgttgggtat tgcgatgatt tgcctgctgt ttattaaaga tatttagctc
     1321 accagtctgg gctaagaaaa atcggcgtgg cgcaaacaga gccatcctcg ccatttttgc
     1381 tatcatgcct gcatacataa acgacaaaac agtatgcaga gggaaaaatg ggttccacca
     1441 gaaaggggat gctgaacgtt ctgattgccg ccgtgttgtg gggaagttca ggggtctgcg
     1501 cgcaatacat catggagcaa agccagatgt cgtcgcagtt tttgactatg acgcgtttga
     1561 tattcgccgg tttgattcta ctgacgctgt catttgttca tggcgataaa atcttttcta
     1621 ttattaacaa tcataaagat gccattagcc tgctgatttt ttccgtggtt ggcgcgctaa
     1681 ctgtacagct cacttttttg ctaaccatcg aaaaatcgaa cgcagccacg gcaacggtgc
     1741 tgcaattcct ctcaccgacg attatcgtcg cctggttctc actggtgcgt aaatcgcgcc
     1801 cgggcattct ggttttctgc gctattttga catcgctggt cgggactttt ttattggtga
     1861 cacacggtaa tccgacgtca ttatcgatct ctcctgccgc gttgttctgg ggcattgcct
     1921 cggcatttgc tgctgcattc tataccacct atccctcaac gctaattgcc cgctatggca
     1981 cgttaccagt cgtcggctgg agtatgctga ttggcggtct gattctgttg cctttttatg
     2041 ccagacaagg aacaaacttt gtcgttaacg gcagtttgat tctggcgttt ttttatttgg
     2101 tggtcattgg tacgtccctg acatttagtc tgtacctgaa aggagcacaa ttaattggcg
     2161 gtccaaaagc cagcattttg agctgtgcag aaccattaag tagcgcgcta ctctctttgc
     2221 tgttgctggg gatcacgttc acattaccgg actggctggg aacgctgctg attctgtcat
     2281 cggtgatttt gatttcaatg gattcccgtc gccgcgccag aaaaataaat cgtccggcgc
     2341 ggcatgagtg agaattacca gccaggcacc gcgccaccgt taaaaatggt ttctgccgct
     2401 ttagcgactt cgggtgattg ataagattgc agaaattcct tcacgttttc tgcattctta
     2461 ttatcttccc gtgccaccaa aatattcaca tacggcgaat tcttatcttc aataaatacg
     2521 ctgtcgtgca ccggagaaag ccctgtctgc tgaatgtaag tggtgctgat aatcgctaca
     2581 tcaactttgg gatcatccag tacgcgcggg agctgcgccc cctcgagttc cataatctgc
     2641 aaatgacgag gattatcggt tatatccagc gcggtaggta ataacccttt tccctctttc
     2701 agagtaatta gcttctcttt ttgcaacagt aaaagtgcgc gaccaaggtt ggtcgggtcg
     2761 ttggggatcg ccactgtcgc gccttctttt atttgcgcca cggttttgat ttttttggaa
     2821 taacccgcca tagggaagac aaacgtattt cccaccgcta ccagtttata accatgcgcc
     2881 tgattatctt gttcaagaaa gggtcgatgc tggaagacat tcgcatcaag ttcgccatga
     2941 tttgtcgcat cgttcggtag caacgaaccg ctaaatccta ccagttcaac atcgaggcca
     3001 tatttctctt ttgccacctt tttagcgact tccgcgacat cttgttctgc gccatttata
     3061 acgccaactt taatgtgttt tgcatcgctg ctactctggt cgcaacctgc cagcagaatt
     3121 ccggccagca ataatgcggc ccctgtccgt agatgatgtg ttgtcagttt cattttttta
     3181 tccttttatt aatgattacc tgactatagc ggtaaggtcg ctgcggttta aaaaacgaaa
     3241 cgctatcgat aagaataaaa aggaataaaa gtggaatata aagtctggca cttcttactg
     3301 accacgcaag cgcgtttcgt acagcatgat gagagcgatg aaagcaaact acacttatgc
     3361 ttcattcgat acacatttgt aaaaggttga atccatgaaa ccaacgacac tactgcttat
     3421 tttcactttt ttcgctatgc caggcatcgt atacgccgag tcgcctttta gctcactgca
     3481 gtccgcaaaa gagaaaacca cggtattaca agatttacgt aaaatttgca cgccacaggc
     3541 gtcattatca gatgaagcgt gggaaaagtt aatgttgtca gatgaaaaca ataaacaaca
     3601 tatacgcgag gccatcgtgg cgatggaacg taataatcag agcaattatt gggaagcgtt
     3661 gggtaaggta gaatgcccgg atatgtaaga acagcaacaa ctactgacca acgcttcttt
     3721 acctgatatc aggatttctt cattttcacc tttgcagtaa ccaacaatgc agtcagcaac
     3781 atcaatgtgc cggacaacat caacggcgaa gtcagaccaa tattatccag cgcataaccg
     3841 ccgattgccg cgccacaggt attagcaagc tgaataaccg ccacctgaat agacccggct
     3901 ttttctgcct gatcggccag cgagcgggtg atccacgttg accagccgac gggaaccaat
     3961 gcaaaagtta gcccccagat aatcgccacg ccggtagcaa cgattttatc gcttccccac
     4021 aacgtcagca ccaacgcact cacagccagt attaacggcg cgcctgctaa ggccagtttt
     4081 accgaacgtt taagaatgaa tgacgaaagc gacgtaccga taaagctggc aatgccaaaa
     4141 ctcaacagca ccagcgttaa gccatccacg ctgaatcccg ccaggttcat atacaccggg
     4201 cgaatatatg tgaagaaagc aaactgcccg gcgaaagaca tgaagatggc gatcatccct
     4261 gccatcacac ccggacgttg taataagcgg aaagtatttt gtttctgatg cgagggttcg
     4321 cctggcagtg aaggcaatga tttgataatc cagaaaatac acagcacgcc catcaccgcc
     4381 gccgcattaa agacattgcg ccaaccgata agctcgccta aaaaactgcc caacggcgcg
     4441 gcaatcacca gcgcaataga aaccgcgccg aagatcaccg acagcgcctt cggcaccgta
     4501 cgcggcggca ccagacgcat ggtcagcgac gccgacatcg cccagaaccc gcccagcgcc
     4561 agccccagac aggcacgacc gattaaaagc aaactgaatg agttagcaaa ggaaaccagc
     4621 aagcaggaga gcgtcagcaa aacggcaaac aaaataacaa cgtagcggcg gtcagtagcc
     4681 tgaattgtct gggtaataaa caaactggca aacattgcca caaaggcggt cacggtcacc
     4741 gattgcccgg caaccccttc cgaaatgcct aaatcctggg ccattggcgt caacaaactg
     4801 acgggcaaaa actcaacgat aatcagacag gcgacacaaa acgccaccga gaaaacggct
     4861 gaccagttcg gtcgggtgat ggtatccgcg ccgcggtttt cggcaataaa ttcactcatg
     4921 gtgttacccg tggcatgttt caggggaaaa gccgaccact ttaacattga aatgtgtgac
     4981 gcatttaacg tttttgcaac ttttacggtg ttgcttgagc tggagttaag atcgaaacgg
     5041 aacagggcaa agattaccgg atgcggcgtg aacgtcttat ccacccggca ggtcaggcgc
     5101 ggtctgtagg ctgataagac gaagcggttt taatgcagcc agtgaacgcc ctcggcaggc
     5161 tgaaagaacg cgttatagcg catctgaaaa gcattaatat cctgcatttc aggttcggtt
     5221 tcgcgcagaa aacccagcgc cagcctgacc tgagcatcgg taatcggcgc ggcaagacgg
     5281 gctttttgta aaatccgatg ccattgcagc aggttctgtt cgctaccatc gacaatgaca
     5341 atttgccaga tgagcaacac ctgcgcggcg ttttgcagat gcgactcatc cgggcgatgc
     5401 aaataacgta aaaattcgtc accggctgac tttcccattt gatcgaccat cgactccacg
     5461 ggtacgacat cgatattcag ccgatcgctg agacggcgaa tcgcctttct ggccccggat
     5521 gtcagcaccc gaaaaccaac cacaaacact accgccagcg tggcgagcat tatccagatc
     5581 atgagttctc ctgcgtaatc agaactcatg atatctggaa accctcgcca gttactaatc
     5641 caccagaata atcttcagtg caaacagagc tgcgaccacc accacacaca ggttcagatc
     5701 gcgccagcgc ccggtgcaca ctttcatgat gcagtacgac ataaagccga gtgcaatccc
     5761 ttcggtgatc gagaaagtaa agggcatcat caccgtggta ataaacgcag gcaccgattc
     5821 ggtaaaatca tcccagttaa cgcgcgccag gctcgacgtc atcagcacgc caacaaagat
     5881 taacgctccg gcggttgcgt aaggaggaac catcgccacc agcggtgaga agaacataac
     5941 taacaggaac ataacgccaa ccacaaccgc agtcagcccc gtgcgaccac cgactgccac
     6001 accagaagta ctttcaatat aggcagtaac agacgaggta ccgataaatg cgcctgccac
     6061 cgaactgacg ctatcaacat acagcgcctt attcatattg gggaatttac cgttactatc
     6121 tattaagccc gctttatcag ttacaccaat taatgttcct gatgaatcaa ataggttgat
     6181 cagcataaag gagaaaatga tgccggcgag ttcaagtgtt aacgcgccgc tcaaatccac
     6241 ttcaccaatg acgccgctaa tatcaggcgg aatggaatag acgccgctaa aatgaacatc
     6301 accgaaaaat aatccgcagc aggaagtgac gacgatggaa accagcaccg cggcatgaaa
     6361 atgacgtgat gacaatacgg ttataataaa aaaacctaat atacctaaca acacgccgtg
     6421 agagcttaaa tcgccaatca tcaccagggt gtctttattg gcgacaataa cgccagtgtt
     6481 ttttaatccc attaaggcaa taaataatcc gattccgctg gtgataccaa tacgtaaact
     6541 taagggaatg ttggagatca tccagtaccg gatacgaaac agcgtgagca aaaatagccc
     6601 aactgcgccc cagaatattg cgcccatccc ggtctgccag gagatgccca tcgcccccac
     6661 gaccacgaag gcaaagaagg cgttcagccc cattgccgga gccagcgcca cgggtaagtt
     6721 agcaaatatc cccatcgcaa tactgccgat accggcaatc aaacaggtgg taacaaacac
     6781 cactttcggg tccatttgtg ccgcgccgag gatttgcggg ttcacaaaaa cgatgtacac
     6841 catggttaaa aaagtggtca tccccgcaat caattctgtg cggacggtgg tcccatgctg
     6901 aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat cggtattgtc
     6961 attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta aaacaaatga
     7021 caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat attctttaat
     7081 aatctcgcga cgt
//
LOCUS       DAPGWD010000196         7037 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPGWD010000196 DAPGWD010000000
VERSION     DAPGWD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.197"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(14..754)
                     /gene="traX"
                     /locus_tag="RHX88_004018"
     CDS             complement(14..754)
                     /gene="traX"
                     /locus_tag="RHX88_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HDV2232644.1"
                     /translation="MTTENTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLMVL
                     DHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWAVFSQFWY
                     YVAGFPWYEGNILFAFAVAAQVLTWCETPSRWRFAGTMLLLAVWLPFSGTSYGIAGLL
                     MLAVSHRLYRAEDRMERLVVVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAG
                     KSLPRFWPGHFFPIFYACHLAVLGILAL"
     gene            complement(774..6044)
                     /gene="traI"
                     /locus_tag="RHX88_004019"
     CDS             complement(774..6044)
                     /gene="traI"
                     /locus_tag="RHX88_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HDV2232645.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGRNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETRFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMTFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHQEVTMMALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHCLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVYGGDRLQAASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTDLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLLESERPRIVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTREIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGCGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETEHRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRAAITEREAALPEGVLREPQRVREAVREI
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            6126..6353
                     /gene="vapB"
                     /locus_tag="RHX88_004020"
     CDS             6126..6353
                     /gene="vapB"
                     /locus_tag="RHX88_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDV2232646.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGNSVSADFMDNREQPGMQERESF"
     gene            6353..6751
                     /gene="vapC"
                     /locus_tag="RHX88_004021"
     CDS             6353..6751
                     /gene="vapC"
                     /locus_tag="RHX88_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDV2232647.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQARPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRIEDWS"
     gene            complement(6760..>7037)
                     /locus_tag="RHX88_004022"
     CDS             complement(6760..>7037)
                     /locus_tag="RHX88_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HDV2232648.1"
                     /translation="SPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQQMQRREEVNTNVHRERGEDVEPGDDF"
BASE COUNT         1408 a         2298 c         1779 g         1552 t
ORIGIN      
        1 agtatcatac caatcacaac gccagaatgc ccagcaccgc cagatgacag gcatagaaaa
       61 tcgggaaaaa atgcccgggc cagaaacggg gtaatgattt ccctgcacac gacaccagac
      121 caacggtcag caccgtcatc acaaggccgg ctacagccgc cgcatcactg gtggcaaggt
      181 taagcgccgg aatcacggcc agcaggcaag cgacaaccac cagacgttcc attctgtctt
      241 ccgcccggta cagccggtgg ctgacagcca gcatcagcag cccggcaatg ccgtaactgg
      301 taccagagaa aggcaaccac actgccagaa gcaacatagt ccctgcgaaa cgccaccggc
      361 tgggcgtttc acaccatgtc agcacctgtg ccgcaacagc aaaggcaaac aggatattcc
      421 cctcatacca gggaaagccg gcaacgtaat accagaactg ggaaaaaact gcccagcccc
      481 ataaccggtt aatggctgac tgccggatat gcgcatggcg ggacagattc agcccccaca
      541 ccagagcaaa cagcggaaag gcaccacgcc cagccagaaa catccacgtc tggtccagat
      601 gcaaaatgcg gtttgcatgg tccagtacca tcagcaccag tgccaccgtt ttgatgatat
      661 cccgctgtcc cggagaccag accagcaacg actgcaacca ggtatcggtc cgggcagcca
      721 gtgaatcgtt acgtgtcgtg ttctctgttg tcatcagcgt gtgtcttcct gtatcagtct
      781 ccgcccaggg ttttctcttt ctgcaggtca cggaccatat cccgctccat ctgctgaagt
      841 cgctcctgca gcagattttc ccgggcaatt tccctgacag cttcccgcac ccgttgaggt
      901 tcacgcagta caccctccgg cagcgcggct tcccgttcag ttatggctgc ccggtcacgc
      961 tcctgcccgg caatctccct gacagcctgc tctgttttgc catccggcag gtcaggtttg
     1021 ttctctgcca ttttacggac aatttcatct gcgcggtgtt ccgtttcacg acggatggcc
     1081 tcttcagcct gccgctgtgc cagcacctct gccgtgaccg gttcgccatt tccggctccc
     1141 ggctggacac tgttgtccgg gatatccccc cacacgcgac caccggtgat ggcaccggga
     1201 ttccacggac gaccttcacc ggcgattctc accaccacac cgctgtcagg attatcacgg
     1261 gcaatccgga caccatcctg catattatca gccagcaggc tctctccgtt acggctgccc
     1321 tgcagggcca cgaactgcgc atccccgcag cctttcaccc gtccttcacc actgaatccc
     1381 cgcagcccgt ttccgtcatc cgtggtcagt gggttcagcc agatacctgc tgacttgccg
     1441 ttacggtcaa acgccggcag tgccacatac ggctgcggat atttgcgccc cggtgcaata
     1501 aaccgtgccg gactgtctcc cccggccagc cccgcctgac ggagaacagc acgccctgcc
     1561 gccacgtccc gcagttcccg cgccgtactg aacagccgct ctgcattcat gacctcccgg
     1621 tccggtttcg gctcaaatac atcgtgggcc gttcctttct gtacggcatt gttaatggca
     1681 tccgtccagc cctgacggtt atcggtgtac acctgcacat gctgcttcat acgcgacagg
     1741 gccacgtagg ctgactcaaa gccggccatc agtttccggt taccttccgt gccttcaagc
     1801 gcaatggcaa aggtttcact tgccccctgc gcaccgtgag cggtgatggc ataggccagg
     1861 tcaatatgtt gctctgcctg ctcctggccg gggcgaatct cccgggtctg ctgtccgtcc
     1921 gacagcgtga cactgtcacc ggaaactgct gtcaccgtcc agacgctgtt ggcaacataa
     1981 ccgcgctccc ggtcactctt cgtgaagcgc atccggtcac cggtccccac cctgatggtg
     2041 tccggggtgt acagtgtgac accttcagcc accgcctccc ggggagaaat cagccgcgtg
     2101 ttaccttccg catcctgcag ggttatcagc ccgtcatcct tactgatacc ggcaatccga
     2161 tgatacacat tatccaccag gacaagtgcg tcccgatgtg tctcccaggt ggagagacga
     2221 cgcagctccc cgtcacgtat attcgctgtg ttcaggacag gcaccatgac ctgctctttc
     2281 cccagctcac cggccttttc ccgtacatca tgaatcatgc tgttcagtac gcgccggtcc
     2341 tcattcaggt gcgtgacaat cagcgtctgc tcccgtgctt ccggtgttct gccggtatag
     2401 tcgcggacaa tggcttcata cagtgtcatg gggacatccg gaaaagcctc gcctttcagc
     2461 atcgccttct gctgcgcttc tgccagtttc gcttcctggc tgtgactgaa ctccgtcacg
     2521 gagtgctccg gtgcccatgc gccctcctga cgtggcacct gagacggttt cacactctca
     2581 agcccggaca gtgccctttc cacatcccgg ttaatcaggc tgtataccgc ctcccgcagt
     2641 tccggcgtct gacgcacaat ctccttcatg atggccacat cggcagcact gcgcgtctgc
     2701 tggagacgga aaggctgacc gggcgcgatg gcctgcagct ggtccgtgtc accactggcc
     2761 accgccctac cgccaccggc cgcaatcagg gcgtatgccc gtgccatgtc ggtattgccc
     2821 accattgagc tctcatcgag caggaacagc gtgttgctga aatccggcgt ttctccgctg
     2881 cgctgctgca gctgcgtgtc atgcagaaag gacgccagtg tttgtgcatc cacgccggcg
     2941 ctgcgcatct caccgaccgc acggtgcgtg ggccccagcc caacgatacg gggacgctca
     3001 ctctccagca gcatgttcac ggctgacatc accgcccgga actgtgtggt cttacccaca
     3061 ccggcatacc cctgcaccac cgtgaaacgg tcggacgttt ccagtatcat gcgggtggcg
     3121 gcacgctgtc ctgatgtcag tttctccatg agttcgccag gtactctctc catcagcggc
     3181 gtgaccgcct ccttaccttc gagaatatgg cgaagaatgc ttttttcagc ctcatacgac
     3241 gcacgggaaa ccagcaggcc tgtgccatag ccttttgcca catccacata cagtaaatca
     3301 ccgcgtttta tctgcgcatt gatttcccct cccaggtcag taaaactggt tccttctgca
     3361 gcaaacgact tcgcctctgt cagcaggtcc accatgctga aggccaggtt tttactttcc
     3421 agcacaggaa gggccagatg aatggcctgc tgtgccggcg tgtgaagccc tgctttctgc
     3481 agactgatag cggtttccag caatgtctca ccggcacgcg cctttatctg ctcagaaacc
     3541 accgtaaagg agggatggcg ggcaagtttt tccgcagtac gggtttcatc cagcgaggaa
     3601 tacagccgga catcacgacc gctgcgggcc agaccgttca gggtggcgtt gtccattgcc
     3661 atctgtgtga cggaggcaaa aaccttcgca ctgtcactga cggaatgccc gggcgtttcc
     3721 acccagccgt tctccagctt cagtgccgtg aacggtgaat cagccacagg cagggtggcc
     3781 ggttcagccc gccccggcac aacaaccgtc atcgcatctt cactgacgga tgccgcctgc
     3841 aggcggtcac cgccgtagac gcggagtccg ggaattttcc ctgtcaccct cagacgctcg
     3901 ccgtctgcca ccggcatttt ttccggccgg aacagcgacc agctgctgtc cagagaagaa
     3961 atacgcacca cctgcgtttc accctgcgca tcccgcaggg tcaggcagtg actctgcgcc
     4021 gtcacccggt ctatcacata gcggtcatga ctgcgtgtct ccgggttcca ctgctccatc
     4081 accattcccg ggcggtacat atcccgcaga taacggctcc ggctgtccag ccagaccggt
     4141 gacagtgcca tcatggtcac ctcctgatga ccgagcacgc cctgtgtttt cagctcgctg
     4201 cgaatggcct gtgtcagtat ggcctgttcc cgtaccccgc tgacctgtgc cacgctctct
     4261 tctccggctt tcacgctggc cgcaaaatct ccggccagcc gggcatagcg gacattacgg
     4321 tctggttcac tgatgatggt ggccggtcgc tgttctcccc cctgccagcg atatgtgttc
     4381 accccggcat ccttcatggc cataagtgca ctgcctgtac cggttcgctg cccgctgtcg
     4441 gttatcagga cctgtacgtt atgacgtgcg gcaccgtcca gcagggttaa cgtctctttc
     4501 agggagagtt tttcgccctg gtcaacgata acggtactgc ccggtgtgaa ggtcatgccc
     4561 tcctgcagct gacgacgtcc ggttatcagc tcaccggaca gccgttcatc ctgcttcagg
     4621 ttcatctgcg aacgacggtc agcagcgata atctgcacct cccgcccctg ctcccgggcc
     4681 atcatgacca gttcagccac ccgctcacgc tgcccggctg caccgccctg cccggacaca
     4741 atggccagcg acgggcgatc ctgtgccaga acactgacgg catcgctgta gccggccgtc
     4801 cgggggacac ttttctccgg atgtacggtc acccggttct gtttcatgat atcgcgactg
     4861 agtgcccgga ctgacagttc atcgagcaca tgaatcccgg acgtgaacag ccccttctca
     4921 cggtcaaggg ggatgagttg ctcacggctg atggcctcat cgataccggc gcgtgcccgt
     4981 tcaatcacac cattttccgg cggcagtata ccgaccgttc tggccagcac gtccgtgtac
     5041 gtaaactgca cttttcgttc acttaatccg gcaatcgcct gtgtcaccgc ctgctgcaca
     5101 tccggcccct cctgtgaaac agccccgggc gcctgcgtgc gggtctccgc acgctgatcc
     5161 gccgcgtcac gatatgcccg gatgtcgaac ctggtttcct tcagcgtctg catccactcg
     5221 gccattctga cttcaggatc gacgtgctgt ttggattttc gcgtatccag cgccgccaca
     5281 tcccgggatt tcagcgaggc gtcttccccg acggcctccc ggatagtctg tgagcgtccg
     5341 gaaaaggcct ccactggtac gcccggcatt tcccacatac cgtgcttacc aaccacttca
     5401 gtttcatagc ccagcgcttc aacctgctct ttcagttttt ctcggtagag cctgccaaag
     5461 gcaatctgat tagcatacac attctcaata aaccccgttt tccccacttt gtcactgctc
     5521 agtgtcttcc actcgccgtt atgctgcgtg acattagcca ccaccgcatg cgtgtgtaac
     5581 tgtggttcct gatcgcgact ggtgtcgtgg ttaaacagtg ccatcaccag attaccggtc
     5641 agcaccgttt ctgactgtcc gtccgtcatc acccgtgtgg aggccagcgc ctccacctga
     5701 cggacagcaa aatccacggc ctggttatgt gcttcaataa gacgtttgtc ccccccgagc
     5761 atggccatca tggagacact tttgggagcg gaaaaggtca ggtcgtagcc gggacgatgc
     5821 ctgttacggc catcctgcat gcggcttaaa tccgccccgt ctggcaacct gccctccaga
     5881 aggcgggtaa agacatcctt atcgacactg ccctgcagcc ccagttgttc agccccctgg
     5941 ccggcccagc gttctcccat gctgcccagc acatagtaat tatccttgtc ggtataatag
     6001 ttcccggcac ttccggccga tctgacctgc gcaatactca tcataaacgt atatcccttt
     6061 gacatatccc ggcatcaatc ccacaatgga tatacacaag acatatccac ataaggaggc
     6121 aaataatgga aaccaccgta tttctcagca accgcagcca ggcggtcaga ctgccaaaag
     6181 cggttgcatt gccggaaaac gtaaaacgcg ttgaagtgat tgctgtcgga cgaacccgaa
     6241 tcattacgcc tgccggagag acatgggacg aatggttcga cggaaacagt gtcagcgctg
     6301 attttatgga taacagggaa cagcctggta tgcaggaaag ggagtcattc tgatgctgaa
     6361 gtttatgctc gataccaaca tctgcatttt tacgataaag aacaaacccg ccagtgtcag
     6421 ggaacgtttt aacctgaacc aggggagaat gtgcatcagt tcggtcactc tgatggagct
     6481 gatatatggt gcagaaaaaa gccagatgcc tgaacgtaat ctcgctgtga tcgagggatt
     6541 tgtttcccgc attgacgttc tggattacga cgctgctgct gccacacaca ccggccagat
     6601 aagagcagaa cttgcccgtc aggcacgccc tgtcgggcca tttgatcaaa tgatcgcagg
     6661 tcatgcccgc agtcggggac tgattattgt gactaataac acccgggaat ttgaacgtgt
     6721 gggcggcctg agaattgaag actggagttg acctgttcat cagaaatcat ctcccggctc
     6781 aacatcctcc ccgcgctccc ggtgcacatt agtgttcacc tcttcacgac gctgcatctg
     6841 ctgctggatg tccggatgat tttcctgttg ccatgcctca taagcggcca tatccaccac
     6901 ttcaccggat tcactgatcc cgggtggcag ttgctgttcc atctcttctt ccggtttcat
     6961 tttcagctcc tgctcgatcc cccctgccgg aacattcaca cctgaatctg acttcttatc
     7021 gttgatggca ggagaca
//
LOCUS       DAPGWD010000197         7018 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPGWD010000197 DAPGWD010000000
VERSION     DAPGWD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.198"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2353
                     /locus_tag="RHX88_004023"
     CDS             <1..2353
                     /locus_tag="RHX88_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2232649.1"
                     /translation="NWTQDYYTLLSSDTGISIADNSHIVQYNVLLTEGAESYVYTSLN
                     DDDNKLISMLRWNNTKGMGYGTFNIEKDATLNIGVSLSDNLSPLLYDGWDGKSLTKSG
                     NGTLILSATNNYTGNTEVKSGVLILAAPDALGRTEYLYLSRGAELDMNGYPQTISKLL
                     TAAGSVLNIHGGSLILNNGGESAGTIAGDGSLNINGGMLDITGNNRNFSGVFTVNKGA
                     HLAVSTADNLGTAFVDNYGTLTLNSTSAWQLTNNISGYGNVRKTGAGALNISDNAKWT
                     GMTDIIQGTVILGNADSPVMLGSNQVIVEEQGKLSGFGGVAGNLSNSGIVDLTTYMPG
                     NILTVGGNYTGRNGLILLQTETGGDNSKTDRLVIKGNASGRTRVAVTQAGGTGAETLN
                     GIEVIHVSGNADNAEFIQTERITAGAYDYILKRGQGINSTNWYLISRKDIPVPQPEAV
                     PENHDNNLRPEAGSYVASIAAANNLFVTNLYERQGQELYISHMTGEENEAGIWMYNKG
                     KHNRWRDNSSQLRTRGNSYVVLIGGDIAQWSLNGTDRWHTGMMAGYGHNNNSTNALST
                     GYHSEGRMNGYTAGLYATWYANDETHNGSYLDSWLQYSWFDNHINGERLPAESWKSKG
                     FTVSLEAGYSWKAGEFTDNYKGSHEWYVQPQLQVVRMNVKSDKYHESNGTSIENTGNG
                     NILTRLGARTWLTSKNGKNTRYAVPFRPFVEAHWLHNSRVFGTSMNGVSIYQDGARDI
                     GEINGGVVGMITPEVAFRADAGIQLGEHGYHNTSAMLSVKYRF"
     gene            complement(2528..2733)
                     /locus_tag="RHX88_004024"
                     /pseudo
     CDS             complement(2528..2733)
                     /locus_tag="RHX88_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2723..2929
                     /locus_tag="RHX88_004025"
     CDS             2723..2929
                     /locus_tag="RHX88_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDV2232650.1"
                     /translation="MAFVSDSDDSYRWNEVDKRFSVDDTPNEPNRFGWVGEIDPYGYF
                     SENNENICALYNQIKPRNKHKYYR"
     gene            complement(3214..3408)
                     /locus_tag="RHX88_004026"
                     /pseudo
     CDS             complement(3214..3408)
                     /locus_tag="RHX88_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3387..3635
                     /locus_tag="RHX88_004027"
                     /pseudo
     CDS             3387..3635
                     /locus_tag="RHX88_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3700..4851
                     /locus_tag="RHX88_004028"
     CDS             3700..4851
                     /locus_tag="RHX88_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HDV2232651.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVLGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            4861..5220
                     /locus_tag="RHX88_004029"
                     /pseudo
     CDS             4861..5220
                     /locus_tag="RHX88_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5321..6477
                     /locus_tag="RHX88_004030"
     CDS             join(5321..5570,5570..6477)
                     /locus_tag="RHX88_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2232652.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTLGSRTVAELESEILQLRKALNEARLERDIFKKSNSVFCTGVAEKYALI
                     EQWRQQFPIEAMCQVFGVSRSGYYNWVQHDPSDRKQSDERLKLEIKVAHISTRETYGT
                     RRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSSHNLPVAPNLLNQTFA
                     PTVPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKAMFMALR
                     SQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE
                     SLSHYRFNSRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT         2047 a         1519 c         1722 g         1730 t
ORIGIN      
        1 gaactggact caggactatt acacattatt gtcgtcagac acaggtatct cgatcgctga
       61 taatagtcac attgtacaat acaatgtatt actgacagaa ggtgctgaaa gttatgttta
      121 tacgtcttta aatgacgacg ataacaaact gatatccatg ctgagatgga ataatacaaa
      181 agggatggga tatggaacct ttaatataga aaaagatgca actctgaaca taggcgtttc
      241 tctttccgat aatctttcac ctttattata tgatggctgg gacggcaaaa gtctgacaaa
      301 atcaggtaat ggtactctta tactttctgc aacaaacaat tatacaggaa atacagaggt
      361 taaatctggc gtattaattc ttgctgcacc tgatgctctt ggtcggactg agtatttata
      421 tttatcccgt ggcgcagaac tggatatgaa tgggtatcct cagacaataa gcaaactact
      481 gacggctgca ggctctgtgc tgaacattca tggcggaagc ctgatactga ataatggagg
      541 agaatctgca ggtactattg caggggatgg ttctctgaac ataaatgggg gaatgcttga
      601 tataacgggt aataatcgta atttttccgg tgtttttacc gtgaataagg gggctcattt
      661 ggctgtatcc acggctgata atctggggac agcctttgtt gataactatg gcacattaac
      721 tctgaacagt acatcagcat ggcagcttac caacaatatc agtggttatg gtaatgttcg
      781 caagacggga gcaggtgcac tgaacattag cgataacgca aaatggaccg ggatgacaga
      841 tattattcag gggacagtga tactggggaa cgcagattca ccggtgatgc tcggcagtaa
      901 ccaggtcatt gttgaagagc agggcaaact ctccgggttt gggggcgttg caggaaatct
      961 gagcaatagt ggtatagtcg atctcactac atatatgccg ggtaatatac tgactgtcgg
     1021 agggaattac actggcagaa atggacttat tctcctccag acagaaacag gtggtgacaa
     1081 ttcgaaaaca gatcgtctgg tgattaaagg taatgccagt ggccgtaccc gtgtcgctgt
     1141 tactcaggcc ggtggtactg gtgcagagac acttaatggg attgaagtga ttcacgtcag
     1201 tggcaatgct gataatgctg aattcattca gacggaacgt attacagccg gagcttatga
     1261 ttacatactg aaacgtggtc aggggattaa cagcactaac tggtatctga ttagcagaaa
     1321 agacattcct gtaccacaac ctgaagctgt accggaaaac catgataata atttgcgtcc
     1381 tgaggcaggt agctatgttg ccagtattgc tgctgcaaat aatctgtttg taacgaatct
     1441 gtatgaacga cagggacagg agttgtatat cagccacatg acaggagaag aaaatgaagc
     1501 aggtatctgg atgtataata aaggaaaaca taatcgctgg cgtgacaaca gtagtcagct
     1561 gagaacccgg gggaatagct acgttgtgtt aataggggga gatatagctc agtggagcct
     1621 gaatggtacc gatcgctggc atacaggtat gatggctggc tatggtcata ataataacag
     1681 tacgaatgcc ctgagcaccg gataccattc ggaaggaaga atgaatggat acacagcggg
     1741 tctttatgca acatggtatg ccaatgatga aacacacaat ggttcttatc ttgatagctg
     1801 gctgcaatac agctggtttg ataatcatat aaatggagaa cggctgcctg ctgagtcatg
     1861 gaagtcaaaa gggttcacgg tatctctgga agcaggatat tcatggaagg ctggagagtt
     1921 taccgacaat tacaagggaa gtcatgaatg gtatgttcag ccgcagcttc aggttgtccg
     1981 gatgaatgta aaatcagaca aatatcatga aagtaacgga accagtattg aaaataccgg
     2041 taacggaaat attctcaccc gcctgggagc aagaacatgg cttaccagca aaaacggtaa
     2101 aaatacgcgg tatgcggttc cgttcagacc atttgtggag gcacactggt tgcacaatag
     2161 tcgtgttttc ggcaccagta tgaatggtgt aagtatatac caggatggtg cgcgtgatat
     2221 cggagaaata aatggtggtg ttgtgggaat gataacacca gaagtagcat tccgggctga
     2281 tgcaggcatt caacttggag aacatggata ccataataca tctgccatgt tgagtgtgaa
     2341 atatcgtttc tgatgtgtct ttacattata aatatccttc acaaaatatt gttgataaga
     2401 catggttagg tggtgagctg cctcggaaaa tatgacgaca gtaatgattt ctaagagatt
     2461 tgaaatgcgt tgataacaaa atctgaaata taggagtctg tttaggattg tgtgtaaatg
     2521 ccttttctca aaagtaaccg tccaggcggt cactgaatcc gataataaag cggcttattg
     2581 ccatacccca gtccttcaac ggcatcgtcc atttcgggga cgatgcctgt attgccagac
     2641 acaccacttt tttcaccggc tcgtctgtcg gaaacactta cgctttttga tcgcatggcg
     2701 gatcacgctg tttagcgacc cgatggcatt cgtcagcgac agcgacgact cttaccgctg
     2761 gaacgaagtg gataagcgct tcagtgtcga cgacacgccg aacgagccga accgcttcgg
     2821 ctgggtgggg gaaatcgatc catatggtta tttttcagaa aataatgaga acatctgtgc
     2881 tttatataac cagataaaac cacgcaataa gcataaatat tacaggtgat aatactttta
     2941 tttatcactt cggttatatt caataaacat aataactttc tcttaaatca caggtccagc
     3001 cattgcagtt gttatcagac gtagattagg atgccagtgc atttacatgg gatggtttgt
     3061 gtatccaccg ggtataatgc gtgatatcat taatttcaac actaactgtg cgattgtctt
     3121 tcatgcctgc cctccgggca ggcttttttc tgcatgcaga aaaaaatcac cggaaaaaac
     3181 ggcagcagat aaaaaagagt gctgacaggg atattatttc atccgctcac tggcgagatg
     3241 ataaacgttg ctacgttcac gtttacctac tgcgactacc gcaataatta acatatcatc
     3301 aataacctga taaacaagac gaatgaatcg ccacaggttt aatagacacc tcagagtcat
     3361 ttaagatgac ttaaagagag gtgcccatga gcggtaagcg ttatcccgaa gagtttaaaa
     3421 ttgaagcagt caaacaggtt gttgatcgcg gttattctgt tgccagcgtt gcaacacgtc
     3481 tcgatatcac cacccacagc ctttactcct ggataaagaa gtacggtccg gattcttcca
     3541 ctaataaaga acagtcagat gctcaggccg agatccgccg tctccagaaa gagctgaagc
     3601 gggttaccga cgaacgggac atattaaaaa aagccgttgt agattcaatt ggtcaacgca
     3661 acagttatgt gaaaacatgg ggttgcagag gttttttgaa tgagacgaac atttacagca
     3721 gaggaaaaag cctctgtttt tgaactatgg aagaacggaa caggcttcag tgaaatagcg
     3781 aatatcctgg gttcaaaacc cggaacgatc ttcactatgt taagggatac tggcggcata
     3841 aaaccccatg agcgtaagcg ggctgtagct cacctgacac tgtctgagcg cgaggagata
     3901 cgagctggtt tgtcagccaa aatgagcatt cgtgcgatag ctactgcgct gaatcgcagt
     3961 ccttcgacga tctcacgtga agttcagcgt aatcggggca gacgctatta caaagctgtt
     4021 gatgctaata accgagccaa cagaatggcg aaaaggccaa aaccgtgctt actggatcaa
     4081 aatttaccat tgcgaaagct tgttctggaa aagctggaga tgaaatggtc tccagagcaa
     4141 atatcaggat ggttaaggcg aacaaaacca cgtcaaaaaa cgctgcgaat atcacctgag
     4201 acaatttata aaacgctgta ctttcgtagc cgtgaagcgc tacaccacct gaatatacag
     4261 catctgcgac ggtcgcatag ccttcgccat ggcaggcgtc atacccgcaa aggcgaaaga
     4321 ggtacgatta acatagtgaa cggaacacca attcacgaac gttcccgaaa tatcgataac
     4381 agacgctctc tggggcattg ggagggcgat ttagtcttag gtacaaaaaa ctctcatata
     4441 gccacacttg tagaccgaaa atcacgttat acgatcatcc ttagactcag gggcaaagat
     4501 tctgtctcag taaatcaggc tcttaccgac aaattcctga gtttaccgtc agaactcaga
     4561 aaatcactga catgggacag aggaatggaa ctggccagac atctagaatt tactgtcagc
     4621 accggcgtta aagtttactt ctgcgatcct cagagtcctt ggcagcgggg aacaaatgag
     4681 aacacaaatg ggctaattcg gcagtacttt cctaaaaaga catgtcttgc ccaatatact
     4741 caacatgaac tagatctggt tgctgctcag ctaaacaaca gaccgagaaa gacactgaag
     4801 ttcaaaacac cgaaagagat aattgaaagg ggtgttgcat tgacagattg aatctacaac
     4861 cgcgctcttg atgtcagact ccctgaacag ttctcgataa tcgggaaact cagagcgcat
     4921 tatcctgtgg tcacactctg ccatgtgttc ggggttcatc gcagcagcta cagatactgg
     4981 aaaaaccgtc ctgaaaaacc agacggcaga cgggctgtat tacgcagtca ggtacttgag
     5041 ctacatggca tcagccacgg ttcggccgga gcaagaagca tcgccacaat ggcaacccgg
     5101 agaggctacc agatgggacg ctggcttgct ggcaggctca tgaaagagct ggggctggtc
     5161 agctgtcagc agccgactca ccggtataaa cgtggtggtc atgaacatgt tgctatgcct
     5221 aaggttctca cgccgccatc atgcagctct gacggatgag gtagcctgag tttaacggac
     5281 actccttcct gaaatagaat ggcatcagaa ggagctaata atgagcagaa aaacccaacg
     5341 ttactctaaa gagttcaaag ccgaagctgt cagaacggtt cttgaaaatc aactttcgat
     5401 cagtgaaggc gcttcccgat tatctcttcc tgaaggcact ttaggacaat gggttactgc
     5461 cgccagaaaa gggctcggta ctcttggttc ccgcacggtg gctgaactgg aatctgaaat
     5521 tctgcaactg cgtaaggcgt taaatgaagc tcgccttgag cgagatatat taaaaaaagc
     5581 aacagcgtat tttgcacagg agtcgctgaa aaatacgcgt taatcgaaca atggcgacaa
     5641 caatttccca ttgaagcgat gtgtcaggta tttggtgtat ccaggagcgg ttattacaac
     5701 tgggtacagc atgacccctc agacagaaaa caaagtgatg agcggctaaa actggagatt
     5761 aaggtggcac atatcagcac tcgcgaaaca tatggaaccc ggcggctcca gacggagctg
     5821 gcagagaatg gcatcatcgt tggtcgtgac cgactggcac gtcttcgtaa ggagctaagg
     5881 ctacgctgta agcagaaacg caagttcaga gcgactacga actcgagcca caatctgcca
     5941 gttgcgccaa atctgctgaa ccagacgttc gctcctacag taccaaatca ggtctgggtg
     6001 gcggacctga cgtatgttgc cacacaggag ggatggttgt acctcgctgg catcaaagat
     6061 gtttatacgt gcgaaattgt cggctacgcc atgggagagc gcatgacaaa agagctgaca
     6121 ggtaaagcca tgtttatggc gctcaggagc cagcgcccac ctgccgggct aatccaccac
     6181 tccgatcgag gttcacagta ctgcgcatac gattaccggg tcatccagga gcagtttggt
     6241 ctgaaaacat caatgtcgcg taaaggtaac tgttacgaca acgctccgat ggaaagcttc
     6301 tggggaacgc tgaaaaatga gagcctgagc cactatcgtt ttaatagccg ggatgaggcc
     6361 atctcagtaa tacgggaata cattgagatt ttctacaatc gtcagcgtcg tcactctcgt
     6421 ctggggaata tctccccggc agccttcagg gaaaaatatc atcagatggc tgcttaaaaa
     6481 aagaacaaat ggtagtgtcc gctattgcca gtacacctca ggatgccgtc cgtctcttct
     6541 gccgctggcc gcagttgcct gcgtttatgc ttcattaatc atcatcgggt tcggatgtcc
     6601 taatcacggt taattatgtt cggcccttca tgctgccggt tttgcagcgc tactacggcg
     6661 tcggtagcac actggcagag cggcagatct cccggggtaa ttcgcacgac tttcctgctt
     6721 atgcctgtcg gctgtacgtc accgcgttcc gtgtgagtat tgggctttgg tgatttgtgc
     6781 cgccttagcc gcttcctgtt cgtcaggcca gcattttgca tccggcttcc ttcagatttc
     6841 acctcgcgat gaacatcctt gccttccgct aacatttccc ctcaccgggt gtttggagaa
     6901 ctttcacctc ttagtcgttc ttcagccacc acagttgaag aacagcgcca gtcacggcgc
     6961 acacaaaaaa cgcgtagtga atacactacg cgtcagactt tttatttttg ttaattat
//
LOCUS       DAPGWD010000198         7004 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPGWD010000198 DAPGWD010000000
VERSION     DAPGWD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.199"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(9..2792)
                     /locus_tag="RHX88_004031"
     CDS             complement(9..2792)
                     /locus_tag="RHX88_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV2232653.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQFSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2849..5221)
                     /locus_tag="RHX88_004032"
     CDS             complement(2849..5221)
                     /locus_tag="RHX88_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDV2232654.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5259..6944)
                     /locus_tag="RHX88_004033"
     CDS             complement(5259..6944)
                     /locus_tag="RHX88_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDV2232655.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1816 a         1600 c         1573 g         2015 t
ORIGIN      
        1 ggctgttatt attttggcaa taatactccg gtataagtat ttaccggatg agaaagatat
       61 tgtttaacgg cagtgttaac attctctacc gtcatttgtt tcaacaattg ctcctgctca
      121 gtccatgctg caggatcgtc atattgaata agactattta caatagtgtt cgctaattgt
      181 tgaacgctac gctgttggat atcgaggctg cgctgaacgt tttgctggta ttcattcagt
      241 tcttgctcac tgatcccttt agccagacgc ttaaccatca cttcattcgc taacgttaac
      301 agttcatcat gtcgttctgg ttgacaagta aaagccagca aatgactgat atctttggcc
      361 tgaggatcaa ccgagaggcg agaagaaacg ctgtatgctc cagatgcctg ttcacgaata
      421 ttaacacgta gatcttttgc cagtgcgacg ttaaaagcat cgagcgccat acgcgtcgcc
      481 agattaacag gtgtccggga atcataacgc ttccactgtg aaaactgtgc cacaggttca
      541 ttttgttctt ttacagtaac cgatgcgttg tccgtcgcgc gagttaatgg tttacctgcg
      601 gctaatggcg aatcagagtg tttgattgat cctaagtaac gcgtaattaa cgccacgagt
      661 ttgtcttctg agacattacc gacaatgaca aacgtgatat ccgctggaga tgaaaacaac
      721 tggcgatcgg cagccagcgc atctgcggca gtaaactgta caatctgatt ttcttgcagt
      781 aatttcgtgc ggtcatcagc atagcgcgtc tcatacatct gctgggcgaa tttctccgct
      841 ggacgctggt cgagcgtttt caacgcctga atttgagcat tttgtagcga tgcccaaata
      901 ttgtcgttaa tcgtgctgtg ggtgattcgc tggttaatca actgaaaacc aggttcaggg
      961 ttattagtcc gcgcgctaac ggagagcaac gtattcatgc cactgacttt actgctcatg
     1021 gttaccgaat tttccgcact ccagcgtttc aggctggaag aggagagctc accaacgccg
     1081 cttccgctta ctgctttatt tgccagcgcg ataagtgatt tttgctgcgc agggaaactt
     1141 aaatcgcctt tattcgatac ggcagtaatt tgtagctttt gctcttcacc cgcggatttt
     1201 gccagaataa ccttggcacc attggaaagt gttaatgatg tcagattctc agccagcgtt
     1261 tctttgctgc taatttccgc ctgtgggtca gcgtctactg ttaacgataa atttctgcct
     1321 gggaagatgt aagccgccag ctttttgttg gcgtactctt tttccagcgc caggatagcc
     1381 gcaggagaca atgctttttt ggcggcaagc tcattgttta ccatttgctc ccaaaatgcg
     1441 tcctggttct ttcttaactg ctgccatttt tccgccagac tttgcacggt aatttgctgc
     1501 cacagacgtt tcgaaagttg atatgtctct tccggcgaca agaacggcgt attatttaat
     1561 gagctggatg ccaggcgact ggtcagcata cgtaaatcac gttcagcttg ctgatcaacc
     1621 gcatttttca gccaggtgag gcgggtagat ttgacatcat cgagttcttc agcagaaaaa
     1681 ccatgctgat caatggttgc caactctgcc attaatgcat tcgcagcatc ctgcatatta
     1741 tcgtctcgtg catttacacg gaaaaacagc gactgataat cgggtgcaat tttaacgctg
     1801 cgcgcagtgc cgccagaaat agtcttcaac tcgcccgact gtatgcgttc ctgcagacgt
     1861 tgattgaaca gctgaactaa catgctccat tcagcttgtt cgacaaagct ttgctcatcg
     1921 ttcacttgta ccattggcag gcgataatag agtgcgatgc cgttcacccg gttttcttta
     1981 tcattaatga tattaaagcg caggtggttt tcggcttttg tcggccagac gcgattttca
     2041 gctgctttgt tagccggaag cttacttaaa ttatccttta tcagcgccag cgcttcttta
     2101 ctgtcgatat cgccgaccac gataaaggtc atattatttg gttgatacca gcgttgataa
     2161 aattggcgca attgtgccgg tgtgaccgtg gcgacagtat ccatcaggcc gataggttca
     2221 cggtctaaat tacgggtatt agccagcagg aaagggcggc gcgcctgaga ggtgcgccat
     2281 ttcgcatcct gatgggcacg ccattcctca gtaattacgc cacgttcagc gtctacttcg
     2341 agtttttcaa aggttgcggc attactccat tcactgaaga ttgccatcac ttgttgcaga
     2401 ttttgtttct gcgtagtcgg caaactcacc tgatacaccg tttcgtcata gctggtatag
     2461 gcattaacat cgcgaccaaa acgcaggccc attgactcaa atgtttcgat gactttatta
     2521 cccggccatg tttttgtgcc gttaaacatc atatgttcta caaaatgagc cacgccgcgc
     2581 tcattgtctt cttcctgcaa tgaaccggta tgaatttgca gccataaatt tacctgatcc
     2641 tttggatgag catgcggata aatcatatat cgcaagccat tgcccagttg cccggtaatt
     2701 aacttttcat cctgcggtaa ggcggcggca atcagccgcc cggggagcaa cagagttgcc
     2761 actaacgtca gtaagaaaca gaggtttctc ataattatct ccatgcgaaa accgggcgaa
     2821 tttacccggt taagtaaaat ccgaactatt aaaatttcat gctgacatca agccagaaag
     2881 tacggccact ggcgtaggtc gccacaccgg tgttcgttgt atcaacagcg gttttgctat
     2941 ccagtacatt gagaatatcg gcactgatcg tcaggttttg ttgtttcaga aattgtggcg
     3001 tccaggacaa gcgggtgtcc caggtcaggc tgctatccag tttttcgtca acatactgct
     3061 tgtaatctga atattcgctg atgtattgcg catttgtctt accaaggata atgcgagctt
     3121 tacgcgcttc ttgccaggcc agcgtattcg cccacactaa cccgctcggt tgatgcgtga
     3181 aatccatgtt taaggagatc tttaatgggt tattaaaatc tgccactgga acgctatcgt
     3241 aagagactag attaccgtta taaaccacct ggttatcacc ggtattgctc tcctcataac
     3301 cattgtttaa cgacaagttg cccttgctct tgatatagct aaagacaatt tgtgggttaa
     3361 tatctacctg gcggatatgc aggggttcgg ccagttcaaa actgaggctg aacgaatgcg
     3421 ttttggtttt gccatcgttg ttatattcag taatggtggt tttagtcgcg ctgtcggtac
     3481 gactgctttt gctgatttga tcatgcgctt cacggtaaac atagtttgcg cgtgcaataa
     3541 cgttcttacc gattttctgc tgcaatccca ttgccagttc atcgttataa ggcgttttca
     3601 aatcctgata acgcgtcaga gttttattac ctgataccga ttccgtccag ctattgcgga
     3661 tatcacgtaa tcccatatca agaatattcc cgccatagta acggttataa cctgctgtaa
     3721 tcattgaggt ttgattagca aaaatatccc attccgtcat aaagcgcggg gagatattgt
     3781 ggtttgacag atagttgtca tagtcatacc gcacgccggg cattaatgac acattacgcc
     3841 agctaatgcg atccgccata tacagcgtgt agttgtcaat tcccaggctg cctttacctt
     3901 tatggtaaat ggtatggtta gttttctttc cggcagcatt aatcacatag gattcagact
     3961 ggttatggcg ttcagtccac gcatcggaat agatgtattc cgcgccgaag tagggttgat
     4021 gcgaaacatt acccacggcg aatttttgcc agtccaggcg tgttttgaag gtgtaattat
     4081 ctacagcctg ggaaatgtgt cctaatccgc cacgggtgca acgccctgta atatcaccat
     4141 atgtacatga aagttcggtg taccagatgt catgatcgtg acgggtataa tcactaatat
     4201 gatcccaacc aacggtggta cgcagtttgg cccatgcgag ctgcgtatcc atatcccatg
     4261 ccagaccgta ggatttatta cccatttcgc gatcagactg cgggaaggtg ctggtattat
     4321 aatcacgact ggagccggta tattttaagg ttaaatcgtg ggtaaagcgg tcgctggcaa
     4381 accaggtaaa tttgctcaat gcagtatcga taacgttttt atactgtgcc cgaccggcga
     4441 caatgccgtc attcgaaaca taatccgcgc gggtgatatc agactggcgg cgcgataaac
     4501 cggcggtaac gccgaagtta tcagcgagtt cctgattaaa cgacaaggta taaaagttct
     4561 ttttaaaatc tggggagtaa taagtacttc ctgaagagcc ttgattaaat gcgctcttgt
     4621 tattctcatc gatatgcgat gttaaccagt ccgaacgcgt agtgcgataa cccaatttca
     4681 ccttgctatc atcagcgttg aagcgtttga tctttgcatc aattaccccg ccattgaagc
     4741 gaccaaattc aaccggcaca aaactgtcat aaagcgtcac attgtccagt aagctgacat
     4801 caagataata cccctgtgac atcccgctaa tattggttgc actactggca tcggactcat
     4861 tcgctgggtt caggttatta gttgcactaa taccgtcaat caaataggca ttctggtagg
     4921 gcgacgcacc gtgaatagag attttctcag ggcgaatatc tccctggttc aacgaggtac
     4981 tttgcgttga atccatgcgt accgcagggt tggttctcag cagatcgctg atattgccat
     5041 tcccggtcgg taaacgctcg atgctttcac tggtataatg cgtgttgccg tttacgggta
     5101 cagggacagg agcatagacc gtcatctctt caaaaaaatg catgtacatg tttttgtcat
     5161 cgacggcctg cgccacatca gcgccacata aaatgacgcc aggaataaga actcgcttca
     5221 tttatttata tctccgtaca gcagggcttt tattgttgtt ataaaaccgt gctaatgtca
     5281 caaatatcat cagccaggtt ccagacaccg ggttgatgtg taaccataat gacgccgctt
     5341 gtgggtagtt tttcacgcac taaacgcagt aagcgtatag cctcttgttc ctcaagatga
     5401 gaggtagttt cgtcaagaaa tatccatttc ggacgtcgta aaattaatcg tgccagggcg
     5461 atacgttgtt tttcgccgct ggaaagaata tctccccagc gatcgtggtc atgaatacgc
     5521 gcagccaatt tccccagacc aacctgatgc agtacttcgc tcaacgattt atcgtctacg
     5581 ggcaggggaa gtgctttaca aataatctct ttcagtaagc cggttttgat taacggtgtt
     5641 tgtgacacat accaactgtc agcaggagaa aaaatatcac ctttaaacca cggccagcag
     5701 tgggataatg ttttaagcag tgtggttttt cccgcaccag agtagccttt cagtaatagc
     5761 catttgcctg gcgaaacatg aaagttcaag ttctctaata tgatcttatt atcaggcgta
     5821 cgaatactcg cattagccac ttgtaccgca tgttggcaat ttttaggctt attcgtaggg
     5881 cgctgttcag tgagttgatg gaactcatac aagcgatcga taaccgcagc cagttcagca
     5941 agttcatcat atttataaat aaaccagctt aaattgttcg atactagcat aaatgcctgg
     6001 cgcgatttca tcagtccgcc cagattgatc tgcccgctaa taaactgcgg caataacaga
     6061 aagtagggaa gaacgctaag cgagcgcgaa taaatatttt gccagtaatc aagccaccgc
     6121 tgacgattca ttaaacgatg ccagttctct ttaatggtat gaaaattgtc gctcaactcc
     6181 tggcgttgta gagactcagc attgcttagc gcaatcagtt ctgcctgctt attatgctgc
     6241 acaagattag ttcgaaatgt cgcttcgcta cgttgttttt ccacattaag cggacgaata
     6301 cgcttaccaa ccttatgggt aaataaagtt ccaccgatca caatgagcac aacggtatag
     6361 accatatacc cctggatatt ccattctgtt ccaccaacag tgaatgagag cgtacccgcg
     6421 ctttgccaaa gaataacggt aaaggtgatc agcatgctaa gtgactggat gaagccaaat
     6481 gaaagactga gcgttttgct gattagtaag agaatgtctt cagcgatacg ttggtcaggg
     6541 ttatctgtat ttttatgctc gccgtagatc tgcgtgaagt agtagttttt atctgcaaac
     6601 cagcgattaa ggtagtaatc tgttaaccat tcgcgccagc gaatagtcag cagtttaatt
     6661 aaccatgttt tgttcacgga tattaataca aagatcccca gcagggcagg gaaccaaagg
     6721 acaagctgcc aaagcttatc ggtttctttc tggcttagcg cattgaaaaa atcattattc
     6781 cagtcgttta accagacctg aattttaacc acgccaagga tcatggcgag gataataatg
     6841 attaacagca ctgaagtttt attattctta cgcagccaaa agggcttcaa cagacagaga
     6901 tactttgcta tcaacataca aaacgtaatg ggaatggtta tcattagcga aaattgattg
     6961 tgccatccaa tgattatcta tggcaatgat ttactgatga aatt
//
LOCUS       DAPGWD010000199         6999 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPGWD010000199 DAPGWD010000000
VERSION     DAPGWD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.200"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            507..1496
                     /gene="araF"
                     /locus_tag="RHX88_004034"
     CDS             507..1496
                     /gene="araF"
                     /locus_tag="RHX88_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDV2232656.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1566..3080
                     /gene="araG"
                     /locus_tag="RHX88_004035"
     CDS             1566..3080
                     /gene="araG"
                     /locus_tag="RHX88_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDV2232657.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3095..4081
                     /gene="araH"
                     /locus_tag="RHX88_004036"
     CDS             3095..4081
                     /gene="araH"
                     /locus_tag="RHX88_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDV2232658.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4248..5048
                     /gene="otsB"
                     /locus_tag="RHX88_004037"
     CDS             4248..5048
                     /gene="otsB"
                     /locus_tag="RHX88_004037"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDV2232659.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAAVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5023..6447
                     /gene="otsA"
                     /locus_tag="RHX88_004038"
     CDS             5023..6447
                     /gene="otsA"
                     /locus_tag="RHX88_004038"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDV2232660.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6454..6882)
                     /gene="uspC"
                     /locus_tag="RHX88_004039"
     CDS             complement(6454..6882)
                     /gene="uspC"
                     /locus_tag="RHX88_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDV2232661.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1766 a         1642 c         1825 g         1766 t
ORIGIN      
        1 agtgatgatt ttataaaacg ttttttcatt tttgcgagat ggctctcatt atacgtgttc
       61 tgttaattaa tgagcacagt gataattaat ggcgaaatga atgacgtaca ttttccacat
      121 ctttgagttg cggttattac accatttcaa aaactcaacg ccaggtaatg cggcctattg
      181 actggttaaa aagaagacat cccgcatggg taccaaagac aacaaggatt tccaggctaa
      241 tcttatggat taatctgctg tgcattcgac aatttgtctg acaaattggc tttcccttat
      301 gtcttttccc gctaaattta tgcacgttct cactgtaatt ctgcgatgtg atattgctct
      361 cctatggaga attaatttct cgctaaaact atgtcaacac ggtcacttat cttttagtta
      421 aaaggtaatg ctttgttttc cgattaattt aacgaatgtc attcgttttt gccctacaca
      481 aaacgacact aaagctggag agaaccatgc acaaatttac taaagccctg gcagccattg
      541 gtctggcagc cgttatgtca caatccgcta tggcggagaa cctgaagctc ggttttctgg
      601 tgaagcaacc ggaagagccg tggttccaga ccgaatggaa gtttgccgat aaagccggga
      661 aggatttagg gtttgaggtt attaagattg ccgtgccgga tggcgaaaaa acattgaacg
      721 cgatcgacag cctggctgcc agtggcgcaa aaggtttcgt tatttgtact ccggacccca
      781 aactcggctc tgccatcgtc gcgaaagcgc gtggctacga tatgaaagtc attgccgtgg
      841 atgaccagtt tgttaacgcc aaaggtaagc caatggatac cgttccgctg gtgatgatgg
      901 cggcgactaa aattggcgaa cgtcagggcc aggaactgta taaagagatg cagaaacgtg
      961 gctgggatgt caaagaaagc gcggtgatgg cgattaccgc caacgaactg gataccgccc
     1021 gccgccgtac tacgggatct atggatgcgc tgaaagcggc cggattcccg gaaaaacaaa
     1081 tttatcaggt acctaccaaa tctaacgaca tcccgggggc atttgacgct gccaactcaa
     1141 tgctggttca acatccggaa gttaaacatt ggctgatcgt cggtatgaac gacagcaccg
     1201 tgctgggcgg cgtacgcgcg acggaaggtc agggctttaa agcggccgat atcatcggca
     1261 ttggcattaa cggtgtggat gcggtgagcg aactgtctaa agcacaggca accggcttct
     1321 acggttccct gctgccaagc ccggacgtac atggctataa atccagcgaa atgctttaca
     1381 actgggtagc aaaagacgtt gaaccgccaa aatttaccga agttaccgac gtggtactga
     1441 tcacgcgtga caactttaaa gaagaactgg agaaaaaagg tttaggcggt aagtaatttg
     1501 ccggaaaaat tcccctctgc atgatgcaga gggggtgtga acgaccagtg attcacggag
     1561 acgttatgca acagtctacc ccgtatctct catttcgcgg catcggtaaa acgtttcccg
     1621 gcgttaaggc gctgacggat attagttttg actgctatgc cggtcaggtt catgcgttga
     1681 tgggtgaaaa tggcgcagga aaatcaactc tcttaaaaat cctcagcggc aactatgcgc
     1741 caaccacggg ttctgtagtg attaatgggc aggaaatgtc cttttccgac acgaccgcag
     1801 cacttaacgc gggcgtggcg attatttacc aggaactgca tctcgtgccg gaaatgaccg
     1861 tcgcggaaaa catctatctc ggccagctgc cgcataaagg cggcattgtg aatcgctcat
     1921 tgctgaatta tgaggcgggt ttacaactta aacatcttgg tatggatatt gacccggaca
     1981 cgccgctgaa atatctctcc attggtcagt ggcagatggt tgaaatcgcc aaagcgctgg
     2041 cgcgtaacgc caaaattatc gcctttgatg agccaaccag ctccctctct gcccgtgaaa
     2101 tcgacaatct tttccgcgtt attcgtgaac tgcgaaaaga ggggcgggtg atcttatacg
     2161 tttctcaccg tatggaagaa atatttgccc tcagcgatgc cattactgtc tttaaagatg
     2221 gacgttatgt caaaaccttt accgatatgc agcaggttga ccacgacgcg ctggtgcagg
     2281 cgatggtcgg gcgcgacatt ggcgatatct acggctggca accgcgtagt tatggcgagg
     2341 agcgcctacg tcttgatgct gtgaaagcac caggcgtgcg tacgccaata agtctggcgg
     2401 ttcgcagtgg tgaaattgtt gggctgtttg gtctggtagg ggcggggcgt agcgaattaa
     2461 tgaaaggcat gtttggcggg acgcaaatca ccgccggtca ggtttatatc gaccaacagc
     2521 cgatcgatat tcgtaaaccg agccacgcca ttgccgcagg catgatgctc tgcccggaag
     2581 atcgcaaagc ggaaggcatt attcccgtgc actccgttcg cgacaatatc aacatcagtg
     2641 ccagacgtaa acatgtgctc ggcggttgtg taatcaacaa cggttgggaa gaaaacaatg
     2701 ccgatcacca cattcgttcg ctcaacatca aaacgccggg cgcggagcaa ctgatcatga
     2761 atctctcagg cggaaatcag caaaaagcca ttctgggccg ctggttatcg gaagagatga
     2821 aggtcatttt gctggatgaa cctacgcgcg gcattgatgt tggcgctaag cacgaaatat
     2881 ataacgtaat ttatgcgctg gcggcgcagg gcgtggcggt gctgtttgcc tccagcgact
     2941 tacctgaagt cctcggcgtt gccgaccgga ttgtggtgat gcgggaaggt gaaatcgccg
     3001 gtgaattgtt acacgagcag gcagatgagc gtcaggcact gagccttgcg atgcctaaag
     3061 tcagccaggc tgttgcctga gtaaggagag tatgatgtct tctgtttcta catcggggtc
     3121 tggcgcacct aagtcgtcat tcagcttcgg gcgtatctgg gatcagtacg gcatgctggt
     3181 ggtgtttgcg gtgctcttta tcgcctgtgc catttttgtc ccaaattttg ccaccttcat
     3241 taatatgaaa gggttgggcc tggcaatttc catgtcgggg atggtggctt gtggcatgtt
     3301 gttctgcctc gcttccggtg actttgacct ttctgtcgcc tccgtaattg cctgtgcggg
     3361 tgtcaccacg gcggtggtta ttaacctgac tgaaagcctg tggattggcg tggcagcggg
     3421 gttgttgctg ggcgttctct gtggcctggt caatggcttt gttatcgcca aactgaaaat
     3481 aaatgctctg atcacgacat tggcaacgat gcagattgtt cgaggtctgg cgtacatcat
     3541 ttcagacggt aaagcggtcg gtatcgaaga tgaaagcttc tttgcccttg gttacgccaa
     3601 ctggttcggt ctgcctgcgc caatctggct caccgtcgcg tgtctgatta tctttggttt
     3661 gctgctgaat aaaaccacct ttggtcgtaa caccctggcg attggcggga acgaagaggc
     3721 cgcgcgtctg gcgggtgtac cggttgttcg caccaaaatt attatctttg ttctctcagg
     3781 cctggtatca gcgatagccg gaattattct ggcttcacgt atgaccagtg ggcagccaat
     3841 gacgtcgatt ggttatgagc tgattgttat ctccgcctgc gttttaggtg gcgtttctct
     3901 gaaaggtggc atcggaaaaa tctcatatgt ggtggcgggt atcttaattt taggcaccgt
     3961 ggaaaacgcc atgaacctgc ttaatatttc tcctttcgcg cagtacgtgg ttcgcggctt
     4021 aatcctgctg gcagcggtga tcttcgaccg ttacaagcaa aaagcgaaac gcactgtctg
     4081 atgctttttt ctgcaacaat ttagcgtttt ttcccaccat agccaaccgc cataacggtt
     4141 ggctgttctt cgttgcaaat ggcgaccccc gtcacactgt ctatacttac atgtctgtaa
     4201 agcgcgttct gcgcaacaca ataagaaaag agaaggagga gaaccgggtg acagaaccgt
     4261 taaccgaaac ccctgaacta tccgcgaaat atgcctggtt ttttgatctc gatggaacgc
     4321 tggcggaaat caaaccgcat cccgatcagg tcgtcgtgcc tgacaacatt ctgcaaggac
     4381 tacagctact ggcaaccgca agtgatggtg cattggcatt gatatcaggg cgctcaatgg
     4441 tggagcttga cgcactggca aaaccttatc gcttcccgtt agcgggcgtg catggggcgg
     4501 agcgccgtga catcaatggt aaaacacata tcgttcatct gccggatgcg attgcgcgtg
     4561 atattagcgt gcaactgcat acggtcatcg cccagtatcc cggtgcggag ctggagacga
     4621 aagggatggc ctttgcgctg cattatcgtc aggctccgca gcatgaagac gcattaatga
     4681 cattagcgca acgtattact cagatctggc cacaaatggc gttacagcag ggaaagtgtg
     4741 ttgtcgagat caaaccgaga ggtaccagta aaggtgaggc gattgcagct tttatgcagg
     4801 aagctccctt tatcgggcga acgcccgtat ttctgggcga tgatttaacc gatgaatctg
     4861 gcttcgcagc cgttaaccgg ctgggcggaa tgtcagtaaa aattggcaca ggtgcaactc
     4921 aggcatcatg gcgactggcg ggtgtgccgg atgtctggag ctggcttgaa atgataacca
     4981 ccgcattaca acaaaaaaga gaaaataaca ggagtgatga ctatgagtcg tttagtcgta
     5041 gtatctaacc ggattgcacc accagacgag cacgccgcca gtgccggtgg ccttgccgtt
     5101 ggcatactgg gggcactgaa agccgcaggc ggactgtggt ttggctggag tggcgaaaca
     5161 gggaatgagg atcagccgct aaaaaaggtg aaaaaaggta acattacgtg ggcctctttt
     5221 aacctcagcg aacaggacct tgacgaatac tacaaccaat tctccaatgc cgttctctgg
     5281 cccgcttttc attatcggct cgatctggtg caatttcagc gtcctgcctg ggacggctat
     5341 ctacgcgtaa atgcgttgct ggcagataaa ttactgccgc tgttgcaaga cgatgacatt
     5401 atctggatcc acgattatca cctgttgcca tttgcgcatg aattacgcaa acggggagtg
     5461 aataatcgca ttggtttctt tctgcatatt cctttcccga caccggaaat cttcaacgcg
     5521 ctgccgacat atgacacctt gcttgaacag ctttgtgatt atgatttgct gggtttccag
     5581 acagaaaacg atcgtctggc gttcctggat tgtctttcta acctgacccg cgtcacgaca
     5641 cgtagcgcaa aaagccatac agcctggggc aaagcatttc gaacagaagt ctacccgatc
     5701 ggcattgaac cgaaagaaat agccaaacag gctgccgggc cactgccgcc aaaactggcg
     5761 caacttaaag cggaactgaa aaacgtacaa aatatctttt ctgtcgaacg gctggattat
     5821 tccaaaggtt tgccagagcg ttttctcgcc tatgaagcgt tgctggaaaa atatccgcag
     5881 catcatggta aaattcgtta tacccagatt gcaccaacgt cgcgtggtga tgtgcaagcc
     5941 tatcaggata ttcgtcatca gctcgaaaat gaagctggac gaattaatgg taaatacggg
     6001 caattaggct ggacgccgct ttattatttg aatcagcatt ttgaccgtaa attactgatg
     6061 aaaatattcc gctactctga cgtgggctta gtgacgccac tgcgtgacgg gatgaacctg
     6121 gtagcaaaag agtatgttgc tgctcaggac ccagccaacc cgggcgttct tgttctttcg
     6181 caatttgcgg gagcggcaaa cgagttaacg tcggcgttaa ttgttaaccc ctacgatcgt
     6241 gacgaagttg cagctgcgct ggatcgtgca ttgactatgt cgctggcgga acgtatttcc
     6301 cgtcatgcag aaatgctgga cgttatcgtg aaaaacgata ttaaccactg gcaggagtgc
     6361 ttcattagcg acctaaagca gatagttccg cgaagcgcgg aaagccagca gcgcgataaa
     6421 gttgctacct ttccaaagct tgcgtaggag cggttaatca cccgtaagtg gaactaacag
     6481 cacatcgacc tcacttgagg caatcactct tttcgccgag caggatgctc gcgaaaagaa
     6541 actgtgattg tgattaccgc aaatcaccaa atcgaaatga tgtttgtgac atacttccag
     6601 aatatgttcg cttaactcac cgtaggcaat aaacgtcttg tcaacgggat accccgcatc
     6661 ctgaattaac ttatcaagaa agctttgcgt ttcttcttgc atcacgctgc gtaaatcttc
     6721 aagcatcggc gcggctaatt gattgtacat ttccgggtcg gaagcgagag taattaaact
     6781 gatgtgtccc tttactggcc tggcgataga taccgctttt gccagcagtt gctgactttc
     6841 cggtgtaacc gcaacagcga caagaatatt gctatagctc ataacctgtt ccttattctg
     6901 tgaacttcag gtgacattaa agctttactc ctaatggaga aacgacgcaa tcccaactcg
     6961 gtcaaacagg taaatattag tctgattatt agtaaagac
//
LOCUS       DAPGWD010000200         6917 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPGWD010000200 DAPGWD010000000
VERSION     DAPGWD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.201"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            20..517
                     /gene="ftnA"
                     /locus_tag="RHX88_004040"
     CDS             20..517
                     /gene="ftnA"
                     /locus_tag="RHX88_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDV2232662.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(555..794)
                     /locus_tag="RHX88_004041"
     CDS             complement(555..794)
                     /locus_tag="RHX88_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDV2232663.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            985..2196
                     /gene="tyrP"
                     /locus_tag="RHX88_004042"
     CDS             985..2196
                     /gene="tyrP"
                     /locus_tag="RHX88_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDV2232664.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2258..2923)
                     /gene="yecA"
                     /locus_tag="RHX88_004043"
     CDS             complement(2258..2923)
                     /gene="yecA"
                     /locus_tag="RHX88_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDV2232665.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3119..3205)
                     /locus_tag="RHX88_004044"
     tRNA            complement(3119..3205)
                     /locus_tag="RHX88_004044"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3169..3171),aa:Leu,seq:taa)
     gene            complement(3218..3291)
                     /locus_tag="RHX88_004045"
     tRNA            complement(3218..3291)
                     /locus_tag="RHX88_004045"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3257..3259),aa:Cys,seq:gca)
     gene            complement(3346..3421)
                     /locus_tag="RHX88_004046"
     tRNA            complement(3346..3421)
                     /locus_tag="RHX88_004046"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3386..3388),aa:Gly,seq:gcc)
     gene            complement(3573..4121)
                     /gene="pgsA"
                     /locus_tag="RHX88_004047"
     CDS             complement(3573..4121)
                     /gene="pgsA"
                     /locus_tag="RHX88_004047"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDV2232666.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4178..6010)
                     /gene="uvrC"
                     /locus_tag="RHX88_004048"
     CDS             complement(4178..6010)
                     /gene="uvrC"
                     /locus_tag="RHX88_004048"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDV2232667.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6007..6663)
                     /gene="uvrY"
                     /locus_tag="RHX88_004049"
     CDS             complement(6007..6663)
                     /gene="uvrY"
                     /locus_tag="RHX88_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDV2232668.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1662 a         1794 c         1648 g         1813 t
ORIGIN      
        1 acctttgtgg agcactatca tgctgaaacc agaaatgatt gaaaaactta atgagcagat
       61 gaacctggaa ctgtactctt cactgcttta tcagcaaatg agcgcctggt gcagctatca
      121 taccttcgaa ggtgctgccg cgttcctgcg ccgtcacgcc caggaagaga tgacgcatat
      181 gcagcgtctg tttgattacc tgactgatac cggcaattta ccgcgtatta ataccgttga
      241 atctccgttt gctgaatatt cctcacttga tgaattattc caggaaacct ataaacacga
      301 acaattaatc acccagaaaa ttaacgaact ggctcatgct gcaatgacca atcaggacta
      361 cccaacattt aatttcctgc aatggtatgt ttctgagcag catgaagaag agaaactgtt
      421 caaatcgatt attgataaat taagcctggc aggcaaaagc ggcgaaggtc tgtattttat
      481 cgacaaagaa ctctctaccc tcgacacaca aaactaatgc tctccggcgg caggtttact
      541 gccgccgttt caaatcagtg acggcaaatc ttactctgat cggtcgaaaa accgtctttc
      601 gaaggtataa atttgccttt tgctgccaga aacgctacca gctctcccgc tgtcatccct
      661 tctgccgagc aggtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat cgcagcttcc
      721 agactggcat gcgtatattg ctcgcctgat tcaatcatca tatttaacac ttcatgaccg
      781 tgaatagagt ccatcgtccc tcctcaaaaa aagcctagcg tagcgattgc cgcttatgaa
      841 gactttgcgc cagcgcagga ctgaatgctt tttattgtac atttatattt acaccatatg
      901 taacgtcggt ttgacgaagc agccgttatg ccttaacctg cgccgcagat atcactcata
      961 aagatcgtca ggacagaaga aagcgtgaaa aacagaaccc tgggaagtgt ttttatcgtg
     1021 gcgggaacca caattggcgc aggcatgctg gcaatgccgc tggctgcggc cggtgttggt
     1081 tttagcgtta cgttaatctt gttgattggg ctttgggcgt tgatgtgcta cacggcgcta
     1141 ttactgctgg aggtgtacca gcatgttccg gcagataccg gtctaggcac gctggcaaaa
     1201 cgctatctgg gacgctacgg tcaatggctg acgggcttca gtatgatgtt cttaatgtat
     1261 gctctgactg cggcatacat cagcggtgcc ggtgaattgt tggcctccag catcagcgac
     1321 tggacaggta tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt tgttgccggt
     1381 ggcgtggttt gtgttggaac atcgctggtc gatttattta accgttttct gttcagcgcc
     1441 aaaattattt ttctggtggt gatgctggta ttgctactac cgcatattca caaagtgaat
     1501 cttttaaccc tgccgttgca acaggggctg gctctgtcag caatcccggt gatttttacc
     1561 tcatttggtt ttcacggtag cgtgccgagt attgtcagct atatggatgg caacgttcgt
     1621 aagctacgct gggtgtttat aaccggtagt gcgatccccc tggtggcata tattttctgg
     1681 caggtggcaa cgcttggcag cattgattca acaaccttta tgggattgct ggctaatcat
     1741 gctggattaa acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc gcatgttgag
     1801 ctggcagtgc atttatttgc tgatttagcc ctcgccacgt catttctcgg cgttgcgtta
     1861 ggcttatttg attatctggc tgatttattt cagcgttcaa ataccgttgg tggacggttg
     1921 caaactggtg caattacctt tctgccgccg ttggcgtttg cactgtttta tccacgagga
     1981 tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg tactggcatt gattatccct
     2041 tcgctgttga cctggcaaag cagaaagcac aatcctcagg cgggttaccg ggtgaaaggt
     2101 ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg cgtgcaattt
     2161 ttgattgcgg cagggttgtt accagaagtg gggtgatcag atagcctcaa attccttatt
     2221 gggtgccaga attaacgctg acacccaatt tgacctctta atgcaggcag cactgcttaa
     2281 atttcttacc actaccgcac gggcaaggat cgttacgccc cggtttctct tctgctttga
     2341 tcggttgctg aacagctttt tcctgcggat gcgccatcca gtacgcatgt agatcaagcg
     2401 ccgccagtcg aatggcatct acgctctctt caaacgcttc tggcgacatt ttctctaccc
     2461 gctcgaagtt ttcctcagta ccgtgcagcg caatcgcctc cagcgctggt tttaacgaat
     2521 cgggcaacgt tgaccagtca gaaagtgcca cgccccgcat atagccaaag caccactcct
     2581 caacaatcgt cagctcgctg ccatcaactt ctcgcaagcc gaataacggc tcaaactgct
     2641 ccgggaattc gttcagacgc tctgcggtat cggccatatg ttgaaaagcc agattcataa
     2701 agcgcgtcat ctctttctct gacgcccagc gcggcacata gtcagcccca ccccacacgg
     2761 caaccagcca ctgtgccggt tcaatctctt gcggagaact caacaccgcc gtcaataaac
     2821 cgtccagctc cgccacatca aggatggcgt ggtcagtgtt gtatttggtc agaatatcgt
     2881 ccagccattc caactcactt tcgtttaacg gtcccgtttt catacgcttt tccttgtgga
     2941 tctcaactcg ccagcaccta tcttacatgt cggtccgtat cagagatact ttttgagtgg
     3001 ctttgctggt gattaaaaat taaggagggt gtaacgacaa gttgcaggca caaaaaaacc
     3061 acccgaaggt ggtttcacga cactgcttat tgctttgatt ttattcttat ctttcccatg
     3121 gtacccggag cgggacttga acccgcacag cgcgaacgcc gagggatttt aaatcccttg
     3181 tgtctaccga ttccaccatc cgggctcggg aagaaagtgg aggcgcgttc cggagtcgaa
     3241 ccggactaga cggatttgca atccgctaca taaccgcttt gttaacgcgc caaattcttc
     3301 aggcctttca gccagacatc cgcttgaggc cgatgtcttt taaactggag cgggaaacga
     3361 gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg
     3421 cattcatcaa gcaatcagtt aatcacttga ttttattatc gtctggcaat cagtgccgcc
     3481 gttcgatgcg ttgcattcta cttacctggc gcgatgagtc aacgatattt ttcaccactt
     3541 ttgatcgttt gctgaaaatt acgccgaaac gatcactgat caagcaaatc tgcacgcgca
     3601 gcgctcaaat attgcaacat tgaccacaga gtcagtaccg cagccacaaa gaaaagtgca
     3661 ataccggcgt actcaaccca aatgttcgga cgccacagca gccaggccaa cgccaccatc
     3721 tgggcagtgg ttttcacttt cccaatccag gagacagcca cgctactgcg tttacccaac
     3781 tccgccatcc attcgcgtag cgcagaaata ataatttcac gggcgatcat cgttgccgcc
     3841 ggtagggtca cccaccagct gtgataatgc tcggttacca gcaccatggc gatagccacg
     3901 agaactttat ctgctacagg gtcaaggaaa gcaccaaacc gggtactctg gttccagcgg
     3961 cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat gagcgcggcg
     4021 gcaaacggcg accaggtgac aggcagataa aagaccaata caaagaatgg gataaggatg
     4081 acacggaaca gtgtaagcaa cgtagggata ttaaattgca taatgacggg taactatctg
     4141 ttgtcagtaa gattacccct atgttgctac agagacatca atgtttcaac gaccagaaga
     4201 tcttttctgc cagaccttgc gaaatacccg gcacttttgc aatttcctcg acgctggcgt
     4261 tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat ttgccgacgt tttggcccga
     4321 cgccttcaat ggtttccagg gagctggtat ttttgacctt cgcccgtttt ttacggtgcc
     4381 cgccaatcgc gtgatcgtgt gattcatcgc gaatatgctg gataacatgc agcgcgggag
     4441 aatctggcgg caaactgaat ccctcacctt ccggctcaaa gaacagtgtt tccagcccag
     4501 ccttacgatc tgctcctttg gcaacgccaa gtagcagtgg atgattttta tcccatgaga
     4561 catccagttc ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg ccgtcgataa
     4621 ggatcacatc cgggatctta ctgtcgtcaa tggctttacc ataacgccga cgcagcacct
     4681 gattcatcgc cgcataatca tcgcccggcg tgatgcctgt aatgttatag cgccgatact
     4741 ccgcacgcag cgggccgtta gcatcaaaca ccacacagga agcgacggtt tgttcgccca
     4801 tggtatggct gatgtcaaag cactccatcc gcttcacttc cggcaatttc aacacgctgg
     4861 caagcgcggt cagtcgctgg tgaacggtag attgctgcga aagtttgctg gttaaggccg
     4921 tcgccgcatt ggtgcgcgcg agtttcagat aacgcgccct atcgccgcga ggtttggttt
     4981 gaacattaat cttgcgtccc gccagttctg aaagggaatc ggcgagcagc gttttatcgc
     5041 taagattaaa atcgagcagg atctcacccg gtaaggtgcg catctggctg ccttgtaaat
     5101 agaactggcc tacgaaggtt tctaccacct cgctcagttc cgtaccgcca ggcactttcg
     5161 ggaaatagct gcggctgccg agcactttgc cctgacgaat gaacaatacg tggacacaag
     5221 ccatgcccgc atcgaacgcc acaccaataa cgtcgaggtc gtcgccggta ttggaaacaa
     5281 actgtttttc ggtgacgcgt cgcaccgcct gaatttggtc gcgaatacgt gccgcttctt
     5341 caaactccag attctggctg gcagtttcca tacggctaat cagttgcgta agcacctgat
     5401 catctttgcc agacaaaaac aggcgcacat actcgacctg ctgagcgtat tcttcttcac
     5461 tcaccagtcc ttcaacgcac ggtcccagac agcgccctat ctgatattgc agacacggac
     5521 gcgagcgatt acgataaaca ctattttcgc actggcgaat ggggaatatc ttttgcagta
     5581 gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg gccgaaatat tcacctttgg
     5641 catgcttcgc accacgatgc atcgccagac gcgggtgggt atcgccactc aggaagataa
     5701 aaggataaga tttatcatcg cgtagcaaaa cgttgtaacg cggctgatag agtttgatgt
     5761 agttgtgttc cagcaacagc gcttcggttt ctgtgtgagt aaccgttaca tcaatttgct
     5821 ggatctgggc gaccagcgct tcggttttgc gcgaagcgag gttgctacgg aaatagctgg
     5881 aaagccgttt tttcaggtct ttcgctttgc cgacatagat aaccgtacca ccagcatcgt
     5941 acatgcgata aacgcctggc tggctggtta cggtttttaa aaacgctttt gcgtcaaact
     6001 gatcactcac tgacttgata atgtctccgc attacacaga ccatggcgaa ttgccaggtg
     6061 agtcagctca acatcgccat gaatgtttag tttactgaac atacgatagc ggtagctgtt
     6121 caccgttttc ggactgagat tgagctgttc tgagatctca ttgaccttct ggcccttggt
     6181 gatcatcagc ataatctgca attcacgttc agacaaactg gcaaatgggc tttctgtttt
     6241 ttctggttcg atctggctta acgccatttg ttgagcgata tcagaagcaa tgtaacgctg
     6301 cccagaatag acagaacgaa tcgcactcac gacttcctgc ggagccgcgc ctttgctgag
     6361 gtagccagca gcacctgcct gcatgacttt cgctggtaaa gggttttctg tatggaccgt
     6421 aagcatgatg attttaacat cagctgtgga acgcgcgatt ttacgcgtcg cctcaagacc
     6481 gccaatgccc ggcatactca tgtccattag caccacgtca acggcgtttg tccggcacca
     6541 cttaacggcg tcttcaccgc acgatgcctc tccgacgact tttataccct ttatatcttc
     6601 cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta gaacgttgat
     6661 caaaggaata tctccagaaa tagggataac gctactgata gttagtcatt cgtatattag
     6721 cggtttttat tgcaactttg aaacgttaaa aatggtgggt attcgatttt tctctcgttt
     6781 ttgtcaattc gattgtccac agttatggtc acgcccgtca ccaacggttt accggatcgt
     6841 ttcacagtaa gccctacgat gaaatgtgac aaaaattgac tttattcagc aaaaatgaaa
     6901 atcagcccgc tgaatat
//
LOCUS       DAPGWD010000201         6874 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPGWD010000201 DAPGWD010000000
VERSION     DAPGWD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.202"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(124..1773)
                     /gene="pgi"
                     /locus_tag="RHX88_004050"
     CDS             complement(124..1773)
                     /gene="pgi"
                     /locus_tag="RHX88_004050"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDV2232669.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2298..3647
                     /gene="lysC"
                     /locus_tag="RHX88_004051"
     CDS             2298..3647
                     /gene="lysC"
                     /locus_tag="RHX88_004051"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDV2232670.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3792..4718
                     /locus_tag="RHX88_004052"
     CDS             3792..4718
                     /locus_tag="RHX88_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDV2232671.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4754..5545)
                     /locus_tag="RHX88_004053"
     CDS             complement(4754..5545)
                     /locus_tag="RHX88_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDV2232672.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVIYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAEQARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5551..6678)
                     /locus_tag="RHX88_004054"
     CDS             complement(5551..6678)
                     /locus_tag="RHX88_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HDV2232673.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1697 a         1783 c         1641 g         1753 t
ORIGIN      
        1 cacgattaaa aatctatttt aggattaacc tcacggtatg atttccgtta aatcacagac
       61 aaggggattt atctgataaa acaatgccga cataacgtcg gcattgttat taaggttttt
      121 aaattaaccg cgccacgctt tatagcggtt aatcagacca ttggtcgagc tatcgtggct
      181 gctgatttct ttatcatctt tcagctctgg cagaatacgg tttgccagtt gtttacccag
      241 ctccacgccc cactggtcga aggtgaagat gttcaggatc acgccctgag taaagatttt
      301 gtgctcatac agcgcaatca acgcacccag gctgaacgga gtgatttcac gcagcaggat
      361 ggagttggtc gggcggttac cttcgaacac tttgaacggc accacgtagt caagcgttgc
      421 cggatcttta ccctgatcac gatattcctg ctcaaccact tcgcgggatt taccaaacgc
      481 cagcgcttcg gtctgggcga agaagttaga cagcagtttc tggtgatgat cagagagcgg
      541 gttatgggtg atagccggag cgatgaaatc gcacggtacc attttggttc cctggtggat
      601 cagctggtag aacgcgtgct gaccgttagt gcctggttca ccccagataa tcgggccagt
      661 ctggtaatcc acaacgttac cgttacggtc aacatactta ccgttggact ccatattgcc
      721 ctgctggaag tacgccgcga aacggtgcat atactggtca tacggcagaa tcgcctcagt
      781 ttccgcacca aagaaattgt tgtaccagat gccaatcagc gccagcagta caggcaggtt
      841 tttctcggca ggcgtggtgg agaaatgctt gtccatcgcg tgtgcgccag aaagcagttc
      901 aacgaagtta tcaaagccga tggagagaac aatcgacagg ccaatcgctg accacaaaga
      961 gtaacggccg ccgacccagt cccagaactc gaacatgttg gcagtatcaa taccaaactc
     1021 gccaacggct ttggcattgg ttgagagcgc cgcaaagtgt ttcgcaacgt gtttttcatc
     1081 acctgccgct ttcaggaacc agtcacgcgc gctatgggcg ttggtcatgg tttcctgagt
     1141 ggtgaaggtt ttagatgcta ccaggaacag cgtggtttcc gggtttactt ttttcagcac
     1201 ttccgcgatg tgagtcccat cgacgttaga aacaaagtgc atgttcaggt gatttttgta
     1261 cggacgcagt gcttcggtca ccatgtacgg gccgaggtca gaaccgccga tcccgatgtt
     1321 cactacgtca gtgattgctt tgccggtata ccccttccac tcaccggaga taatcgcttc
     1381 cgagaaggtt ttcatcttct ccagcaccgc gttgacttcc ggcattacgt ctttgccatc
     1441 aaccagaatc ggggtattgc tacggttacg cagcgctacg tgcagcacgg cgcggttttc
     1501 agtgcggttg atcttctcgc cagagaacat cgacttaatc gcgcccgcca gatcgcactc
     1561 tttcgccaga tcctgtaatt tcgccagcgt ctcttcagtg atgcggtttt tggagtaatc
     1621 caccagcatc tgatcgccga aggttgcgga gaacttagaa aaacgatcgc cgtctttagc
     1681 aaaaagatcg gcgatcgtaa cgtctttcat ttcatcgaag tgtttctgta gtgcctgcca
     1741 ggcagcggtc tgcgttggat tgatgttttt cattagcaat actcttctga ttttgagaat
     1801 tgtgactttg gaagattgta gcgccagtca cagaaaaatg tgatggtttt agtgccgtta
     1861 gcgtaatgtt gagtgtaaac ccttagcgca gtgaagcatt tattagctga actactgacc
     1921 gccaggagtg gatgaaaaat ccgcatgacc ccgtcgttga caaccgcccc gctcaccctt
     1981 tatttataaa tgtactacct gcgctagcgc aggccagaag aggcgcgttg cccaagtaac
     2041 ggtgttggag gagccagtcc tgtgataaca cctgaggggg tgcatcgccg aggtgattga
     2101 acggctggcc acgttcatca tcggctacag gggctgaatc ccctgggttg tcaccagaag
     2161 cgttcgcagt cgggcgtttc gcaagtggtg gagcacttct gggtgaaaat agtagcgaag
     2221 tatcgctctg cgcccacccg tcttccgctc ttcccttgtg ccaaggctga aaatggatcc
     2281 cctgacacga ggtagttatg tctgaaattg ttgtctccaa atttggcggt accagcgtag
     2341 ctgattttga cgccatgaac cgcagcgctg atattgtgct ttctgatgcc aacgtgcgtt
     2401 tagtagtcct ctcggcttct gctggtatca ctaatctgct ggtcgcttta gcggaagggc
     2461 tggaacctgg cgagcgattc gaaaaactcg acgctattcg caacatccag tttgccattc
     2521 tggaacgtct gcgttacccg aacgttatcc gtgaagagat tgaacgtctg ctggagaaca
     2581 ttactgttct ggcagaagcg gcggcgctgg caacgtctcc ggcgctgaca gatgagctgg
     2641 tcagccacgg cgagctgatg tcgaccctgc tgtttgtcga aatcctgcgc gaacgcgatg
     2701 ttcaggcaca gtggtttgat gtacgtaaag tgatgcgtac caacgaccga tttggtcgtg
     2761 cagagccaga tgtagccgcg ctggcggaac tggccgcgct gcagctgctc ccacgcctca
     2821 atgacggctt agtgatcacc cagggattta tcggtagcga aaataaaggt cgtacaacga
     2881 cgcttggccg tggaggcagc gattatacgg cagccttgct ggcggaggct ttacacgcat
     2941 ctcgtgttga tatctggacc gacgtcccgg gcatctacac caccgatcca cgcgtagttt
     3001 ccgcagcaaa acgcattgat gaaatcgcgt ttgccgaagc ggcagagatg gcaacttttg
     3061 gtgcaaaagt actgcatccg gcaacgttgc tacccgcagt acgcagcgat atcccggtct
     3121 ttgtcggctc cagcaaagac ccacgcgcag gtggtacgct ggtgtgcaat aaaactgaaa
     3181 atccgccgct gttccgcgcg ctggcgcttc gtcgcaatca gactctgctc actttgcaca
     3241 gcctgaatat gctgcattct cgcggcttcc tcgcggaagt tttcggcatc ctcgcgcggc
     3301 ataatatttc ggtagactta atcaccacgt cagaagtgag cgtggcatta acccttgata
     3361 ccaccggttc aacctccact ggcgatacgt tgctgacgca atctctgctg atggagcttt
     3421 ccgcactgtg ccgggtggaa gtagaagaag gtctggcgct ggtcgcgttg attggcaatg
     3481 acctgtcaaa agcctgcggc gttggcaaag aggtattcgg cgtactggaa ccgttcaaca
     3541 ttcgcatgat ttgttacggc gcatccagcc ataacctgtg cttcctggtg cccggcgaag
     3601 atgccgagca ggtggtgcag aagctgcatt ttaatttgtt tgagtaatat ttccctactg
     3661 gcgtataacg ctcaccgatt gttatacgcc atcctcactg aaaacataga ttaaataata
     3721 tctgaaaagc aaaatcactc ctgtagaaca gtattataat caccgaaaaa tatcatttaa
     3781 caggagtgtt aatggctact ttaatggaaa aagatgcact tttaaacgga gccagtcagt
     3841 gcatcgcgtt tttatcaaat atcattgata attgctcagt ttcgtcgcat caggattcag
     3901 gcgatgctct caagcgatta gtcagctatc gcgattatct ttattctacc cccgctgaac
     3961 ttgttgattt tacacaaggt aagattctcc ttcagcaggt aagaactcaa tatcagcatg
     4021 aattcaacaa tactacgcat tcagaaaata aagcatcttt tgattctatt tggcagcgtc
     4081 tgacaaatca tgaagtcaca ccgcaacagc atcccattgg ttttgtttta ggtgggcaac
     4141 ccggagcagg taaatcatct ctgattgaat tagccaaaag agagacaaaa gataatataa
     4201 tgattattaa cggtgatgat tttcgatttc tacaccctga ttttaattat atctaccaaa
     4261 attatgggga tgattttgtt acccatacag cgaaattttc cggcgaaacg gtcgaacgtg
     4321 caattgagag agcgatagtc agtaaactca acattgttgt cgaaggaaca tttcgtaatg
     4381 ccgcgacgcc gttacaaaca ttaaaaaaac tcaaagatgc aggctaccaa acagaagtca
     4441 tgataaaaac aacctcagcg gcattaagct gggaaagcac caatgaacgg tatagtaagg
     4501 acaaagaggc gggaaatatt gcccgaaaag tggataaaaa ccaccatgat atcgtcaccg
     4561 gtttgctggc agaaaatgcc cgcaaggtgt ttgcttcgaa cctgtcagat aagtttgcgg
     4621 tgtattcgcg tgagaagatg atatttagtt cacaagcggc aacgaatgac gatattgcaa
     4681 cacttataca gaatgaaatc agcgggaata cacaataata ataagagctg atggcgtgaa
     4741 ataacgccgg gctttatgcc cggcgcatta ccgtctggta cattaagcta tccagttcct
     4801 taacgtcccc ttctctgcta tccggtaaca gcggtggaat ttccccacgc gcctgttccg
     4861 cacgaataaa cgcatgtaac aatggacggc gcggaccata ttccgcttct cctgcgcgct
     4921 gtttacgttc cagtaattcg tctatttctg cacgtaacgg cgcatcaagt tcgcttcccg
     4981 ccagtaactc agcaaagcgc attggcggta cgcctttacc cgcttcgacc cagcgcacgg
     5041 ccagcagcgg acgcagcacg taaaagtatt ttttcagtcg aacttcgtcg ccctgcaaat
     5101 atccggggaa gtttttctgc gccatcgaat agtaatgcca gcgcgcacgc agcggagaaa
     5161 accacattgg gaccattgcc ttaaacgccg ttatcgtttc ttcatcttgt tgataaatca
     5221 ccggagaatc cagccattct atcagcgtcg ggttcgcgcc tttcaataat cccagcgcct
     5281 tacgccactc ccaaccggaa acatctaact cgtcgtcgat gggcagctca ataacatcgc
     5341 gcggtgactc tactcgcaga taccattcca gcgggtgaac atacaaaaac cgtacatcgt
     5401 agtcgctgtc gggcgaggca aatccccagc cgcggctccc tgattcacag gcgtacagta
     5461 ctttcacatc ataccgccgt tcaatttctt ttaactgctg cgataccctt tcgcgcatcg
     5521 cagcactgac tccatttaat ggcatgttac tcaccctttg atattaacca acccatgcag
     5581 aacctgatcc agaccgccaa atacagagat gttatcgatc cgctcagcca ctctctccag
     5641 cgtttctagc tcttttaaac gcaacgctac cggattgttc tccatgactt tggcggtgtt
     5701 cagtaacgag cgcgtcgctg ccgtttcttc acgtcggcga atcacattcg cctgcgcaga
     5761 tttctctgcc tcaaccagct gcgcgaggat atttttcata tcaccaggca ggacgatatc
     5821 tttcacccct aatgaggcaa tctccatacc aaatggcaac atgcgcgatt tcacctgttc
     5881 gctgacaacg tcatcaatga cctgtttatc ttccagcagt tcatccagcg tacgagtacc
     5941 taccgcttca cgcagggcaa attgcaattc ccgatacaaa tgatcaatcg gcttcgttaa
     6001 ctgactaaat gccagcaaca cgtcgctgta gcgccagtta gcggcgaggt taatccgcag
     6061 attgacctta tctttcgtta agatttcttg cccgctgact tccagtacct gcaagcgggt
     6121 gtcaaccact tcagcgtcaa ccagatggtt aattttccag tatgcggtca gccccggcgg
     6181 taacagcgcc tgcgtttcac catcgatttt cagcacgcct acatgccagg ccggaacctg
     6241 caccgttagt attgcatcgc ggcctttaac tgcccctttg cgccgtggtt gcaataccgc
     6301 attcatcacc tccgtttgta cctggacttg ctgcgtattc atccgcacca gtgtcaaatc
     6361 atcctctaca cgccagtaca aacggcgcgt tgatggcggc aggatctcct gcaaaatacc
     6421 gtccatatac aacgccccgg cttcaatctc cgacaagtct gccaccaggc aatatttctc
     6481 cacccaatca ggctgaaagc ggcgcaaata atccgccagc acgtcgggaa cttcgctgcc
     6541 atcgagcgta atcagcaaga cttctggcgt atttaaccag ggtaaaagat gctcgcctgc
     6601 atgtagcact ttgtagtaat cgccgtttct ggagagtaat gccagctgat cttttcttac
     6661 gcttattttt ttaatcatta tctattcctt gcttaacagc cgtcgcggaa gcagacacga
     6721 agcgacaggg gaattccctt ttgcctgtta cccggcatct ttatcggcga cgttatcggg
     6781 gatcgctgac tgcgcaatcc ctcacttttt atttaataag ttaaaaccag tattcatttc
     6841 aggaatgaat gtgggaatcg aacccataat tggc
//
LOCUS       DAPGWD010000202         6760 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPGWD010000202 DAPGWD010000000
VERSION     DAPGWD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.204"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            199..702
                     /gene="dps"
                     /locus_tag="RHX88_004055"
                     /gene_synonym="pexB"
     CDS             199..702
                     /gene="dps"
                     /locus_tag="RHX88_004055"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDV2232674.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1106..1852
                     /gene="glnH"
                     /locus_tag="RHX88_004056"
     CDS             1106..1852
                     /gene="glnH"
                     /locus_tag="RHX88_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDV2232675.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1991..2650
                     /gene="glnP"
                     /locus_tag="RHX88_004057"
     CDS             1991..2650
                     /gene="glnP"
                     /locus_tag="RHX88_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDV2232676.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2647..3369
                     /gene="glnQ"
                     /locus_tag="RHX88_004058"
     CDS             2647..3369
                     /gene="glnQ"
                     /locus_tag="RHX88_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDV2232677.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3486..5711
                     /gene="ybiO"
                     /locus_tag="RHX88_004059"
     CDS             3486..5711
                     /gene="ybiO"
                     /locus_tag="RHX88_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDV2232678.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(5708..6634)
                     /gene="rlmF"
                     /locus_tag="RHX88_004060"
     CDS             complement(5708..6634)
                     /gene="rlmF"
                     /locus_tag="RHX88_004060"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDV2232679.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
BASE COUNT         1598 a         1729 c         1733 g         1700 t
ORIGIN      
        1 ccgaaaattc ctggcgagca gataaataag aattgttctt atcaatatat ctaactcatt
       61 gaatctttat tagttttgtt tttcacgctt gttaccacta ttagtgtgat aggaacagcc
      121 agaatagcgg aacacatagc cggtgctata cttagtctcg ttaattactg ggacataaca
      181 tcaagaggat atgaaattat gagtaccgct aaattagtta aatcaaaagc gaccaatctg
      241 ctttataccc gcaacgatgt ctccgacagc gagaaaaaag caacagtaga gttgctgaat
      301 cgccaggtta tccagtttat tgatctttct ttgattacca aacaagcgca ctggaacatg
      361 cgcggcgcta acttcattgc cgtacatgaa atgctggatg gcttccgcac cgcactgatc
      421 gatcatctgg ataccatggc agaacgtgca gtgcagctgg gcggtgtagc tctggggacc
      481 actcaagtta tcaacagcaa aaccccgctg aaaagttacc cgctggacat ccacaacgtt
      541 caggatcacc tgaaagaact ggctgaccgt tacgcaatcg tcgctaatga cgtacgcaaa
      601 gcgattggcg aagcgaaaga tgacgacacc gcagatatcc tgaccgccgc gtctcgcgac
      661 ctggataaat tcctgtggtt tatcgagtct aacatcgaat aaatccatcg ctgatggtgc
      721 agaactttag tacccgataa aagcggcttc ctgacaggag gccgttttgt tttgcagccc
      781 acctcaacgc acttatttag tgcatccatc tgctatctcc agctgattaa gtaaattttt
      841 tgtatccaca tcatcacaca atcgttacat aaagattgtt ttttcatcag gttttacgct
      901 aaataatcac tgtgttgagt gcacaatttt agcgcaccag attggtgccc cagaatggtg
      961 catcttcagg gtattgccct ataaatcgtg catcacgttt ttgccgcatc tcgaaaaatc
     1021 aatgagttgc aaaactggca cgattttttc atatatgtga atgtcacgca ggggatcgtc
     1081 ccgtggatag aaaaaaggaa atgctatgaa gtctgtatta aaagtttcac tggctgcact
     1141 gaccctggct tttgcggttt cttctcatgc cgcggataaa aaattagttg tcgcgacgga
     1201 taccgccttc gttccgtttg aatttaaaca gggcgataaa tatgtgggct ttgacgttga
     1261 tctgtgggcg gccatcgcta aagagctgaa gctggattac gaactgaagc cgatggattt
     1321 cagtgggatc attccggcac tgcaaaccaa aaacgtcgat ctggcgctgg cgggcattac
     1381 catcaccgac gagcgtaaaa aagcgatcga tttctctgac ggctactaca aaagcggcct
     1441 gttagtgatg gtgaaagcta acaataacga tgtgaaaagc gtgaaagatc tcgacgggaa
     1501 agtggttgct gtgaagagcg gtactggctc cgttgattac gcgaaagcaa acatcaaaac
     1561 taaagatctg cgtcagttcc cgaacatcga taacgcctat atggaactgg gcaccaaccg
     1621 cgcagacgcc gttctgcacg atacgccaaa cattctgtac ttcatcaaaa ccgccggtaa
     1681 cggtcagttc aaagcggtag gtgactctct ggaagcgcag caatacggta ttgcgttccc
     1741 gaaaggtagc gacgagctgc gtgacaaagt caacggcgcg ttgaaaaccc tgcgcgagaa
     1801 cggaacttac aacgaaatct acaaaaaatg gttcggtact gaaccgaaat aataacgcta
     1861 cacctgtaaa acgcactggc agttccctct cccctctggg gagaggatta gggtgagggg
     1921 cgcaaacccg ctccggggcc attaattacc ctgaatttga ttatttacac cacggtaaca
     1981 ggaacaacat atgcagtttg actggagtgc catctggcct gccattccgc ttctgattga
     2041 aggtgccaaa atgaccctgt ggatttcggt cctcggtctg gcaggcggtc tggtaatcgg
     2101 attgctggca ggttttgcac gcaccttcgg tggttggata gccaaccacg tcgcgctggt
     2161 ctttattgaa gtgatccgcg gcacacctat cgtcgtccag gtgatgttta tctatttcgc
     2221 cctgccgatg gcgtttaacg acttacgcat cgacccattt actgcggcgg tggtcaccat
     2281 catgatcaac tccggcgcgt acattgcgga aatcacgcgt ggtgcggtgc tgtctattca
     2341 caaaggtttt cgtgaagcag gactggcgct cggtctttca cgttgggaaa ccattcgcta
     2401 cgtcatttta ccgctggcac tgcgtcgtat gctgccgccg ctgggtaacc agtggatcat
     2461 cagcattaaa gacacctcgc tgtttattgt tatcggcgtg gcggaactga cccgtcaggg
     2521 gcaagaaatt attgccggta acttccgcgc ccttgagatc tggagcgccg tggcggtgtt
     2581 ctatctgatt attaccctgg tgctgagctt tattctgcgt cgtctggaaa gaaggatgaa
     2641 aatcctgtga ttgaatttaa aaacgtctcc aagcattttg gtccaaccca ggtgctgcac
     2701 aatatcgatt tgaacattgc tcagggcgaa gtcgtggtga ttatcgggcc gtccggttcc
     2761 ggtaaatcga ccctgctgcg ctgcatcaac aaactggaag aaatcacctc cggcgatctg
     2821 attgtcgatg gcctgaaggt taacgatccg aaagttgacg agcgcctgat tcgccaggaa
     2881 gcgggtatgg tgttccagca gttttacctc ttcccgcatc tgacggcgct ggaaaacgtc
     2941 atgtttggtc cgctacgcgt gcgtggcgcg aacaaagaag aggcggaaaa actggcgcgt
     3001 gagctgctgg cgaaagtcgg tctggcagaa cgtgcacatc actacccttc cgaactttct
     3061 ggtggtcaac agcagcgtgt ggcgattgcc cgcgcgctgg cggtgaagcc gaaaatgatg
     3121 ctgtttgatg aaccgacttc cgctcttgac ccggaactgc gccatgaagt gctgaaggtt
     3181 atgcaggatc tggctgaaga agggatgacg atggtgatcg tgacccacga aatcggtttt
     3241 gccgagaaag tagcttcgcg gctgatcttt atcgacaaag gccggattgc ggaagatggc
     3301 gatccgcagg tattgatcaa gaacccgccg agccagcgct tgcaggaatt tttgcagcac
     3361 gtctcttaac ttctctgcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat
     3421 ttaaaatcgc ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag
     3481 gagtcatgcg gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg
     3541 tatccatacc cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg
     3601 aaccggatat cgaacaaaaa aaagcggcct atggcgcact ggcggatgtg ctggataacg
     3661 acacctcgcg taaagaattg atcgaccagt tgcgtactgt cgccgcgacg ccccctgcgg
     3721 aaccggtccc gaagatcgtg ccgccgacgc tggttgaaga gcaaaccgtg ctgcaaaaag
     3781 tcaccgaagt cagccgccat tatggtgaag ccctttccgc ccgcttcgga caactttatc
     3841 gcaatatcac cggctccccg cataagccgt ttaatccaca aaccttcagc aatgcgttga
     3901 cccatttttc aatgttagcg gtattagtgt ttggttttta ctggctgatt cgtctgtgcg
     3961 cactgccgct gtatcgcaaa atgggccagt gggcgcggca aaaaaatcgc gaacgcagca
     4021 actggctgca gcttccggcg atgattatcg gggcgtttat tatcgaccta ctgttactgg
     4081 cactgacatt gtttgtcggc caggtattaa gcgacaacct gaatgcgggc agtcgcacca
     4141 tcgctttcca acaaagtttg tttctcaacg cctttgccct gattgaattt ttcaaagccg
     4201 tactacgcct gattttttgc ccaaacgtgg ctgaactgcg cccgttcacg attcaggacg
     4261 agagcgcccg ttactggagt cgtcgcctga gctggttaag cagtttgata ggttatggcc
     4321 tgattgtggc cgtgccgatt atctctaatc aggtgaatgt acagataggt gcgctggcga
     4381 acgtcatcat tatgctgtgc atgaccgtct gggcgttgta cctgatcttt cgtaataaaa
     4441 aagagattac ccagcatttg ctcaactttg cggagcattc gctggccttt ttcagcctgt
     4501 ttatccgcgc ctttgcgctg gtgtggcact ggctggcaag cgcctatttt atcgtgctgt
     4561 ttttcttttc gttgttcgat ccgggcaaca gcctgaaatt tatgatgggt gcaacggtgc
     4621 gcagcctggc gattattggt attgcagcgt ttgtttccgg tatgttttcc cgctggctgg
     4681 caaaaaccat cactctctcg ccacatactc agcgtaacta tccagagttg caaaaacggc
     4741 tgaatggctg gctttcggcg gcgctgaaaa cggcgcgtat tctgaccgtc tgcgtggcgg
     4801 taatgttgct gttgagcgca tggggattgt tcgatttctg gaactggctg caaaacggcg
     4861 cggggcagaa aaccgtagat atcctgatcc gtatcgcact tattcttttt ttctcggcgg
     4921 ttggctggac ggtgctcgcc agtttgatcg aaaaccggct ggcttcggat attcatggcc
     4981 gcccgctacc cagcgcccgt acgcgtaccc tgctgacgct gttccgtaac gcgctggcgg
     5041 tgattatcag taccatcacc atcatgattg tgttgtcgga aatcggcgtc aatatcgcgc
     5101 cattgctggc aggtgccggg gcattaggtc tggctatctc gtttggttcg caaacgctgg
     5161 tgaaagatat tatcaccggg gtatttattc agtttgaaaa cggcatgaac actggagatt
     5221 tggttactat cgggccgttg accggcactg tggaacggat gtcgattcgc tccgtgggcg
     5281 tgcgtcagga taccggggcg tatcacatca ttccttggtc ttcgataacc acctttgcta
     5341 acttcgtccg cggcattggt tcggtggtgg caaactacga tgttgatcgc catgaagatg
     5401 ctgataaagc caatcaggca ctgaaagatg cggtagcgga attgatggaa aacgaagaaa
     5461 ttcgcgggct ggttattggt gaaccaaact ttgccgggat tgtcggctta agcaataccg
     5521 cgtttacact gcgtgtttcg ttcaccacgc tgccactcaa acagtggacg gtccgctttg
     5581 ccctcgacag ccaggtgaaa aagcatttcg acctggcagg tgttcgcgcg ccagtgcaga
     5641 cttatcaggt gctgcctgct ccgggcgcgg ccccggctga accgctgccg ccgggggagc
     5701 caacgcttta acgttggcga ttaacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc
     5761 aggcaataaa gcgactctgc ttttgcccct gggccatctc ttttttaacc accttcaccg
     5821 cgcccacgtc cgtcagagca cgatacaacg gcggtaagtt ttcaccacga gaaaccagcg
     5881 aggtaaacca catcacctgc ttcgcgaagc ctttgctctc ttcaatcatc tttttgataa
     5941 aggcgacttc accaccttca caccacaact cctgttgctg gccgccaaag ttcagtgcat
     6001 cgtctttgtt cagccccagg ttacggcgtt tacgctcact acctgcccgt gccgcagcgg
     6061 cggaatcgtg gaatggcggg ttacacaagg tcgcgtcgta ttgctcgttt ttatggatga
     6121 tgccgttaaa aatcgcccca ctctcttttt gccgacgcag acgaatggcg cggttaagcc
     6181 ccggattagc actgatgatc gcctgcgcac tacttaacgc ctgactgctg gtttcactac
     6241 cggtaaaacg ccagccatat tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa
     6301 cgccgatatc cagaatgctg gcattagctg gaattgttcc gcttgcttct gccagtaaat
     6361 cggcaaggtg atgaatataa tccgcccggc ccggtactgg cgggcagaga aaaccgtccg
     6421 ggatatccca gttcgctacg gcgtaaaaat gggccagcaa tgccttattg agcgccttca
     6481 ccgccagcgg attggcaaag tctacgcttt gctcccctgc gggtgtaagc gtgaggaatt
     6541 gcctgagttc aggattgacc tgacaaagcg tggcgagatc gtagcggctg tgatgacggt
     6601 tgcgcggatg caaccccggt ttctgggcgg acatagcatt ctcctttaag cagcggcgta
     6661 agatacccgc ataacgcgac agggtaaata tctggctgcg tttatcattc cccaaaacct
     6721 taacatggct atggtttatg gcaattcatc ataaaaatca
//
LOCUS       DAPGWD010000203         6758 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPGWD010000203 DAPGWD010000000
VERSION     DAPGWD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.205"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(131..616)
                     /gene="sixA"
                     /locus_tag="RHX88_004061"
     CDS             complement(131..616)
                     /gene="sixA"
                     /locus_tag="RHX88_004061"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDV2232680.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(819..2963)
                     /gene="fadJ"
                     /locus_tag="RHX88_004062"
     CDS             complement(819..2963)
                     /gene="fadJ"
                     /locus_tag="RHX88_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDV2232681.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2963..4273)
                     /gene="fadI"
                     /locus_tag="RHX88_004063"
     CDS             complement(2963..4273)
                     /gene="fadI"
                     /locus_tag="RHX88_004063"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDV2232682.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQGTAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4453..4737)
                     /locus_tag="RHX88_004064"
     CDS             complement(4453..4737)
                     /locus_tag="RHX88_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDV2232683.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5109..6449
                     /gene="fadL"
                     /locus_tag="RHX88_004065"
     CDS             5109..6449
                     /gene="fadL"
                     /locus_tag="RHX88_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDV2232684.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1675 a         1826 c         1612 g         1645 t
ORIGIN      
        1 aatgaatgta gaaagtgttt tatttataga taaggagagt cttcgatagt agtgaggttg
       61 acagatatcc gctgcctgat gagcaattat gcccaatgaa tctacctcat tgggcataat
      121 ttggaacaca tcagatagct tttgccatct tcagattaca cgggctcatc tgccagttga
      181 atgtaccgtt tccgctttca tcaagcgtta cgctggcaat ggccgaggtg gtaaacatag
      241 gcggcgtttc gcccggacat aactcggcaa ccagataccc gactaacggt aagtgagaga
      301 tcaccagtac cgaagcaaca ccttcattgg tcaacgcctg caaataggca ctgaccagac
      361 caacatcgcc gcagggcgtt aactccggca gaacttctgc actggaaggc aggttcagac
      421 aatcccctac ttcttccagt gtttgctcgg ctcgcaggaa cgggctcacc agaacgcgtt
      481 cgatttccac tttttgacct ttcagccagt tcgccatcag gcgagattcg tcacaaccat
      541 tagtggtcag aggacgaacg gaatcactgg cggcatcgag ggctgcgtcg ccgtgacgca
      601 tgataaaaac ttgcatattg caccgctttt gttaaccaga ttcgcccgtt ttctttcatc
      661 tgaaaccaga gaaagaaaac ggtggccggc attgtgcctt atccattcac cgaatgaaac
      721 gctgtttttt acctcaatgg cgtaagtata gtcaatatgc gtttacattt tgaccaacac
      781 tccgccattc agcgcggatt catatagctt tgaccttctt attgcaggtc agttgcagtt
      841 gttttccaaa aactttcccc acgcgcgccc atctcgacca aacgctcgca aggggtaaaa
      901 cgggaaccat actgcgtggc aagtcgttgc attattgcaa ccacttcgcc cgcgccgaga
      961 gaatcgatat agcggaacgg tccaccgaga aatggcggaa aaccaatgcc aaataccgca
     1021 ccaatatccc cgtcacgcac gctacggata acctgctcat caacacaacg tactgcttca
     1081 ttcagcatca acatcacaca ccgttcagca acctgcggtg cggagagtcg cccctgccct
     1141 tgtgcgccaa tgagcgggta aatggcggga tcgacctgtt ttttgctttt acgccctttc
     1201 tgaccataaa gatagaaacc ccgaccattt tttctgcctt tgcgatcgtc gttcaaaatt
     1261 gaagaaacaa catttgcagg cgcgctaaaa cgttctccat aagcggcttc cagtacagga
     1321 ataattttag tcccggtgtc gattcctacc tcatccaaaa gttggattgg acctaccgga
     1381 aaaccaaatt tcactagcgc ggcatcaatg tgctctaccc gttcaccttc ggtcagcatg
     1441 cggatagctt cattaatgta aggcgctaag atgcgattga cgtaaaaccc ggctttgtca
     1501 cgcacgacaa tgggcgtttt gccctgtttt ttcgccagtt ttaccgtagt ggcgatggtt
     1561 tgcgctgatg tacccgcatg aggaataatc tccaccagcg gcattttttc caccggactg
     1621 aagaaatgca ggccgataac ttgctcaggt cgcgtggcgt gagcggcgat atcaccaatc
     1681 ggcaaagatg acgtattcga agcaaagatg gtatgagcgg cgcaattttg ctccacttcc
     1741 gccaccatct gttgttttaa ttcgagattt tcaaacaccg cttcaataat cagatcgcga
     1801 tgggcaaagc cgcgatagtc cgtcgttccg gagattaatg ccagctgttt gtcacgttcg
     1861 ctggctttga gatgacgacg gcgaactttg ccctccagct gatcccaact gtacttcagc
     1921 gcatgattta tgccctgcgg gttgatatct ttaattctga ccggaagccc cgctttacaa
     1981 gcagtgacat aagcaatacc gccgcccatc aagccaccac ctaaaattcc cacgctgttt
     2041 aatggcgcag gcggtgcatc actaccggga tctttcttca cgtccgtact ggcaaaaaag
     2101 atactacgca gcgcctgcga ttgtggcgtc atcgccagtt cgccaaacgc ccgagcttcg
     2161 gcgtcataac cgctgctggt gccctgcgct aatcccgttt caacaacctc caggatgcgt
     2221 tctgtcgccg gataattgcc ttgagttttg tgttctgttt tcttgccgac cattttgaac
     2281 agcagcgcac gacctaacgg ccccgccaga atacgctcgc gtacaggtag agggcgggaa
     2341 gatgggcgat ccttcttcgc cagctcaaca gcgacttcca gcaaaatgga gtgcggaaca
     2401 acgtcatcca ccagccccag ctttaatgcc tgtttcgccc gaagttgttt tccggtgagg
     2461 atcatctcta atgctgtgct gacgcctatc agacgcggta aacgctgggt gccgcctgaa
     2521 ccgggtaaca atccaagttg tacttcaggc aaaccgagca ccgttttagg atcgtcagta
     2581 caaacgcgac cgtggcacgc cagcgccaac tccagcccac cacccaggca agcgccatga
     2641 atagccgcga taaccggaat gggcaaagca tgaatctccg ccatcaactg ttggccctgc
     2701 cgcgccagtg cttccgcttc ttgcgccgtt ttgcagttgc cgatcatgtt gatgtctgcg
     2761 ccagcaatga agttgtccgg tttagcggag acaaacacca cgcctcgcaa ctctttgttt
     2821 tcacggagtt gcttaataat ggcgcgcacc tgcgaggcaa actccgcctt cagggtattc
     2881 attttctcac ccggtacgtc gatggtgatg atagcaatgt tgtccagacg aacattaaga
     2941 gtaaacactg atgccatttc cattattccg cctccagaac cattgccgcg ccaagcccac
     3001 cggcagcaca ggcggtaact aaaccaaatc caccgccgcg acggcgaagt tcatgcaatg
     3061 tctgggtaat catccgcgcg ccggtcgccg cgaagggatg cccgtaagca atcgaaccgc
     3121 caagcacgtt aaatttgcta tcgtccactt cgccagtggc atgtgcacgc cccagtactt
     3181 cacgagcaaa acgttcacta cccagcaact gaatattcgc cagtgtctga gctgcaaagg
     3241 cttcgtgcat atcgatcaat gtcagatcgc tcattgtcaa accggcacgt tccagcgcca
     3301 gcggtgttga ccaggccgga ccgagcaaca tgtcctgcca gacatcaatc gcagtaaatg
     3361 cgtagctgcg cagatacccc agcggcacca gccctaattc tttcgcccgg gattcagtca
     3421 tcaggatcac cgccgctgcg ccatcggtca gcggcgtact gttcgccgcc gttaccgttc
     3481 cgtgtttgcg atcaaacgcc gggcgcagct ttgcgtaatc ggcaagcgag gaattaccgc
     3541 gaatattgtt gtcttcgaca agcggttgtt tataaggagg gataaaggca gtcatcacct
     3601 cttctttgag tttaccgtct gaccacgcct gagcggcacg ctgatgcgaa cggtgcgcta
     3661 atgcatcttg ctgttctcgg gtgatgccgt aggttttcgc catttgttcg gcggtgtcgc
     3721 ccatccgcaa gccagtagaa tattctgcta ccgcaggcgg tacgggcatt aagtcgcgca
     3781 aacgcaggcg agagaagagt ttcagtcgct ggctcatggt tcgagctttg ttgacatcaa
     3841 ccagcacgcg cgccagtttt ttactgacgc caattggcaa tacggaagag gaatctgccc
     3901 caccggcaat ccccgctcgg atagttcccg ccatcaggct ttctgcgacg tttgcaaccg
     3961 cctggaaact ggtagcgcaa gcgcggctga cgctgtaagc atcggtatgc acattcattc
     4021 cggtaccgag aacaatttca cgcgcaatgt tgggggcttc aggcatttgt acgacctgac
     4081 caaagaccag ctgttcaatc acttcagcgg ggatctcgct gcgtgccagc agttcgccta
     4141 ccaccatctt ccctaaatca accgcgggaa tgccatgaaa agccgtcccc tggcgggcaa
     4201 aaggcgtacg taaaccgcta acaatggcga tacgatcgcc ctggcgggta accagcggta
     4261 aaacctgacc cataacactc ccctgtaaaa aaataaataa agtggtctga cctgatcata
     4321 gtcttaacca tttttttaca tttagccaag cggagaaaaa agaaagtggg agctatgaca
     4381 cagtgaagac aggagaaaga aaagaaaacg cccctgccag caactgacag gggcgaacgt
     4441 acccgagagg aattaacgca gacccagctg gaagatcagc atttcagctt cgcaggcaaa
     4501 agtgaaatcg atatccaggc gcacaccgtc agactcttca gtgaaagtcg gggagatttt
     4561 gcacggttcg gattccacgc tacgggcttt ttcagtcagc gccgccagcg tttgttctgc
     4621 ttctgcgcgg tttgcaaaca cgcggctgta agacgcggtg cagtcggagt tgtccataat
     4681 ggtgccaaca tccatacagc agcaaaccgg ggtttcatca gcactacatt tactcatcgt
     4741 tgatttcctc tgtatgtgca cccaaggtgc cagataaacg ttgtggatat tttacgcttc
     4801 cggaaagtgc tgctccagtt gttaattctg caaaatcgga taagtgaccg aaatcacact
     4861 taaaaatgat ctaaaacaaa attcacccga atccgcgagt gcgcaacctc caaattttgc
     4921 cagctggatc gcgtttctta gatcatattt gaaaaaagat ggaaacatac ttgcaacatt
     4981 ccagctggtc cgacctatac tctcgccact ggtctgattt ctaagatgta cctcagaccc
     5041 tacacttcgc gctcctgtta cagcacgtaa catagtttgt ataaaaataa atcattgagg
     5101 ttatggtcat gagccagaaa accctgttta caaagtctgc tctcgcagtc gcagtggcac
     5161 ttatctccac ccaggcctgg tcggcaggct ttcagttaaa cgaattttct tcctctggct
     5221 tgggccgggc ttattcaggg gaaggcgcaa ttgccgatga tgcaggtaac gttagccgta
     5281 accccgcatt gattaccatg tttgaccgcc cgactttttc tgcgggtgcg gtttatattg
     5341 acccggatgt gaatatcagc ggaacgtctc cgtctggtcg tagcctgaaa gccgataaca
     5401 tcgcgcctac ggcatgggtt ccgaacatgc actttgttgc accgattaac gaccaatttg
     5461 gttggggcgc ttctattacc tctaactatg gtctggcaac agagtttaat gatacttatg
     5521 caggcggctc tgtcgggggt acaactgacc ttgaaaccat gaacctgaac ttaagcggtg
     5581 cgtatcgctt aaataatgca tggagctttg gtcttggttt caacgccgtc tacgctcgcg
     5641 cgaaaattga acgtttcgca ggcgatctgg ggcagttggt tgctggtcag attatgcaat
     5701 ctcctgccgg gcaaactcct caagggcaag cattggcagc taccgccaac ggtatcgaca
     5761 gtaataccaa aatcgctcat ctgaacggta accagtgggg ctttggctgg aacgccggaa
     5821 tcctgtatga actggataaa aataaccgct atgcactgac ctaccgttct gaagtgaaaa
     5881 ttgacttcaa aggtaactac agcagcgatc ttaatcgtgc gtttaataac tacggtttgc
     5941 caattcctac cgcgacaggt ggcgcaacgc aatcgggtta tctgacgctg aacctgcctg
     6001 aaatgtggga agtgtcaggt tataaccgtg ttgatccaca gtgggcgatt cactatagcc
     6061 tggcttacac cagctggagt cagttccagc agctgaaagc gacctcaacc agtggcgaca
     6121 cgctgttcca gaaacatgaa ggctttaaag atgcttaccg catcgcgttg ggtaccactt
     6181 attactacga tgataactgg accttccgta ccggtatcgc ctttgatgac agcccagttc
     6241 cggctcagaa tcgttctatc tccattccgg accaggaccg tttctggctg agtgcaggta
     6301 cgacttacgc atttaataaa gatgcttcag tcgacgttgg tgtttcttat atgcacggtc
     6361 agagcgtgaa aattaacgaa ggcccatacc agttcgagtc tgaaggtaaa gcctggctgt
     6421 tcggtactaa ctttaactac gcgttctgat aacgcgttcg cctggataaa gtcacctgca
     6481 tagcaggtga ctttaactcc cccacttcac cgaaagtagt gtcctcattg cttacctcct
     6541 gagttttgca aacagcctgt tggcagcttg ccctttcaaa tcaataagcg gtatccacag
     6601 acaaacctga agggaaaggc atattttcag gcgttctgct cgtccttctc aaagagttta
     6661 cttttctgca tttccaggat actcaacccc cctggctatt gtgcgctcat acactcaaat
     6721 taaagatagg ttctaaataa atgagcgttt tttgatag
//
LOCUS       DAPGWD010000204         6646 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPGWD010000204 DAPGWD010000000
VERSION     DAPGWD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.206"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..546
                     /locus_tag="RHX88_004066"
     CDS             <1..546
                     /locus_tag="RHX88_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232685.1"
                     /translation="INKDNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQR
                     ITTFFQTSPDVKNREIRLEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDE
                     IQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIV
                     ISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(789..1484)
                     /locus_tag="RHX88_004067"
     CDS             complement(789..1484)
                     /locus_tag="RHX88_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDV2232686.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVMISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1477..2904)
                     /locus_tag="RHX88_004068"
     CDS             complement(1477..2904)
                     /locus_tag="RHX88_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDV2232687.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2915..3634)
                     /locus_tag="RHX88_004069"
     CDS             complement(2915..3634)
                     /locus_tag="RHX88_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDV2232688.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4161..5015)
                     /gene="rclR"
                     /locus_tag="RHX88_004070"
     CDS             complement(4161..5015)
                     /gene="rclR"
                     /locus_tag="RHX88_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDV2232689.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5241..6566
                     /locus_tag="RHX88_004071"
     CDS             5241..6566
                     /locus_tag="RHX88_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDV2232690.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHNENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1816 a         1640 c         1449 g         1741 t
ORIGIN      
        1 atcaataaag acaatataaa agtgatttac agcctaaaaa taaaccagac aacgccaggt
       61 atacttgtta gctgtgatag caataataat tttgcctgtc agaccacaat gactgaagat
      121 gttattcagc gaattactac attttttcag accagcccag atgtcaagaa cagagaaata
      181 aggctggaat ggtcaggaga taagagagct ttaccaactg ctgaagagga aatatctcgc
      241 gtgcaggcct ctattatcaa atggtatgcg tcagaatatc ataatggcag gcaagttctc
      301 gatgagatac aaacgccttc agcaattaac agtgagcttt atacaaaaat gatatacctg
      361 accaggaact ggtcattgta tccgaacggt gatggctgtg taactataag ctcaccagaa
      421 ataaaaaata aataccctgc tgccatttgc ctggctctgg gattttttct aagcattgta
      481 atttctgtaa tgttttgcct tgtcaaaaaa atggtagatg aataccaaca aaactctggg
      541 cagtaataaa caacattctg atagagagtg cctgtataaa gctgaagtga caatgaccgg
      601 gaagggaacc cgttttcagg aatggatgcc tgtctttttc actgtaaata tcctggcatt
      661 actaatgcca ggatatttac agaagagcag cataaccatg acggaacggg gttaagtcag
      721 gttgtcctta acgctatttt caacctcgta tgccttcttc aggtttatgt tcagatctca
      781 tatctctctc agcaatcctc aataatcaga tacaccgctt tcaccggacc atgaaccccg
      841 acgactttga taagctcaat atccgccgtt gaactagggc cgctaatgat gttaatgcag
      901 gacggcattc gttcaccggc ctgcgctttc tgatgcaatt tttctgcgag ttgcgctaca
      961 cgcggcaaga tagtgctttt acgcaggata aaaagagaag attccgggag caggctcaat
     1021 gaacgcccgc gcccggcggc ggaaaaaaga accacgcctc ccgattcggt taaaccatat
     1081 tcagcataca cgacacccac tttagcctgc tcggcctgcg agatattctc ggcacctttc
     1141 gccggatccc aaacaacggc attgcattcc tgctgcaaac gttcactaat ccccaattcc
     1201 tccagcctcg tgtcaccgct aatcatgacc gactgatctc ccagctcttt acacagacgt
     1261 attgcagctt ctgccgcctt cgcctcgctg gtcagctcac agcgcgtcaa cataacatcg
     1321 ctggcaaact gaataaacgc gtcacagcgc tgctgttggt taagttgggt aagccgctcg
     1381 ttagcatagt tgttaagcgg cgcatcttct gcttgcggtt caagtcgcag cgggcgaccc
     1441 agtgcctgag caacgttatt caaaaattcg ctccgattat ccattctttt tctcctgcgc
     1501 ctgatgtttc ttaaaccaac tacggaaact ctctccgtca gcttcaggaa gatcgcgtgc
     1561 ttccatccag tcgctaatcg cgccaaattt gagtggtgtt ttgccgccat tgataaacca
     1621 gcttgccgca tgagcaccgg ccatcatccc gactttccac aatcctggat gactattggc
     1681 ataagcgaac atttttatcg cccgttgctc tgcttttgcg gtgatccctt tttcagccat
     1741 cacccgacga tgacgcaaaa tcagttttga cagcggaata cgcaccggac acacgctgtc
     1801 acaagcagtg cataaagagc aggcgtaggg taaatcttta aaatctttat agccgccaag
     1861 tagcggagaa atcaccgcac caattggccc tggataaata gagccatatc catgaccgcc
     1921 aatatggcga tatgccggac aagtattcat acaagccccg cagcgaatac agcgcagcac
     1981 atcccgaaat tcagaggcca gcacctcaga acgcccgtta tcgacaataa ccagatgaaa
     2041 ctcttcagga ccatcaacgt gccccgcttc gcgcggtcct gtcagccagg tgttgtatcc
     2101 cgtcaaacgt gcaccaacgg cactgcgcgc cagcatggtg atcaatacat ctacctcggc
     2161 aaacgtgggg gcaatacgct ccattcccat cactgcaata tgcgttttag gcagcgtggt
     2221 acacattcgc gcattacctt cattggtcac caggcatacc gaacctgtct ctgccaccgc
     2281 gaaattacag ccggtaatac ctatttcagc actgaggaaa tcttcgcgga ttttttgccg
     2341 gatgaataag gtcatcgctt caggcgtttc cggcccctca tagcccagac gttcgtgtag
     2401 cactcgacgg atctgatggc gatctttatg aattgccggg accacaacat gagatggcgg
     2461 atcttgatcc agctggagaa tatattcacc cagatcggtt tcaatcacct gaatgccagc
     2521 atcctgcaac acatgattga caccaatctc ttcggtcacc atcgatttag atttcaccac
     2581 cttccgggca tttttgcgtt gggcaacctg taaaatgtag cgggtagcgt cttctttggt
     2641 ttttgcaaac cagacgtgac cgccgttttg cgtcactttt tctgagagct ggtacagata
     2701 agcgtcgaga ttactcagaa catgatcacg tatctgggcg gcccgatcgc gccactcctc
     2761 ccagtgcccc aattcatcga ccattttttg ccgatttgcc ccaatacgct gctgcgcgtt
     2821 tgccaccgct ttgcgcatga tcggatcttc aatttgctga cggatgcgtg tcttaaaatc
     2881 tgtattactg gttttgatcg acatctttat atcctcagcg gctcatcaac acttcagcaa
     2941 tatgcatcac tttgactttc tgcccttccc gttgtaatcg cccactgatg ttcagcaggc
     3001 aactcacgtc agcaccaatt aaatactcag ggcggacttc catcaggtgc acaacctttt
     3061 ctttcaccat ctcgccggat atttcggcca ttttgaccga gaacgtgccg ccaaatccgc
     3121 agcaggtatc ctgttcagca aaggtcaaca gctccagtcc acgcacattt ttcagcagcg
     3181 taagtggctc gtccttcact cccagcttac gggccaggct acaagatggg tgatacacag
     3241 ctctcccttg caaactggca cctacatcga ctacccctaa tttattaaca ataaaagagg
     3301 tgagatcctg catacgcgcg gcaacctttt cggcacgtga tgcccattca ggttcatccg
     3361 ccagatacgt cgggtaactt tttacggcat aggtgcaaga gccagccggt gaaataatgg
     3421 gatcgtcgtt atcctccagt gcggcgatca gatttttcat ccctggaatc gcttctttga
     3481 tataaccgct attgatcgca ggctgaccgc agcatccctg tttctccggg aaatttacgc
     3541 gacagccgag tttttccagt agcagcacgg agtctcgtgc cattcttgat ttcagggcgt
     3601 caccaataca ggtgacaaag aaattgacat tcacaactaa tactccatta cttcatgccc
     3661 atttatgccc gatgtttccc ccccaccggg ggatcaatca tcaataaata tggcgttctt
     3721 ttttaattgg ctagcaacat accacccctc atttgtatga caaatgatga tctttcatcc
     3781 aaacttcaaa tgcaataact aattttattt ctttcggcat cgctcacatt ttatggggaa
     3841 atcgacgttc atttttggca taagaaatcc atttaaaaag gctacatccc ttatacctat
     3901 tcagagctac ttattcaggg gttattgaga tatacactga tggtgtacaa aaaaatcacc
     3961 gacaataatc acgtatcaag taatagtcgg ggttttattt aattgaagca atttagtttg
     4021 catttttatt taaaaactga gctagagtcc atcagaaaaa aatatataaa gcccaaacca
     4081 ccgttggaaa tagaaatagt tgaaaggctc acaactcccg gaatgcggat tatctttgcc
     4141 ctgatttctg gcggttttat tcagggtgca agctgtctga ccctttcccg atattctccc
     4201 ggagtacaac caaactcgcg gacaaacgcc ttgtgaaaag atgattcact ggcatagcct
     4261 actgactcag cgatcaccac aacagggagc atttcccggg aaaacatctg ggccgctatt
     4321 tgtagacgca actttgttaa tacagccagc ggcgtggttc cggaaacatc acggaaaagc
     4381 tgggcaaaac ttgcccggga catgtgagcg atgctggcca gcgattcgac ggtccaggcg
     4441 tgtcctggca tttccagcat ttgctgtatt accgcaccaa gacgtggatg cagaagcaaa
     4501 ctgagaatgt ttttctcagt attaacctgt gcaatccact cacgcaccgc gagggtaaag
     4561 aatgtagcgc agatctggct acacagtgca tccactcccg gcatcgccga tctggattcc
     4621 tgttgtaaaa acgggatcgc ctccctcagc cagttatatt ccacgctgtg gttaaccggt
     4681 gctaaaaaca gcgtttccgg caaagacgtt aaaaaataac gcgccgaatg ttgcaaccga
     4741 agagtgccgc agacaataca ggtcgattca ttatcgacat gactcaggcg atgagctgaa
     4801 ttttgtggta gcaggaccac atttcccggg cgtaatgtaa aaatctctcc cgtcggcatt
     4861 tccagcttcg ccgctccttg cgttaacgca tgccaacgaa taaccgataa ttccccggca
     4921 ccatgtggaa gctgccagtc acttcctaac acgcaattct tatcgatcgt tccttgtgga
     4981 gcgttaagca tcaacaaacg gctaagggca tccatatcag actcctgagt ggtttttgat
     5041 tacaaacacc aacaatctta gtaaataact aatctaatac catcaaatgg ttaatcatga
     5101 tttttgtttt gtgattctga ttaagaaaaa gaaaatacca gacgataaga caaaatatca
     5161 agcgttgtga agaaatggta tttgctcttt tgcgtctata gtcatgatgt caaatgaacg
     5221 cgtttcgaca ggaaatcatc atgaataaat atcaggcagt gattattggt tttggcaagg
     5281 ctggaaaaac attagccgtc acgctggcaa aagcaggttg gcgagtggct ctcatcgaac
     5341 aatcaaatgc aatgtatggc gggacctgta ttaatatcgg ctgcatccca accaaaacat
     5401 tggttcatga cgcacagcag cacacagatt ttgtccgtgc catacagcgt aaaaatgaag
     5461 tggttaattt tttacgtaat aagaattttc ataatcttgc ggatatgccc aatatcgacg
     5521 tgatcgacgg ccaggcggag tttatcaata atcatagcct gcgtgttcat cggcctgagg
     5581 gaaatctgga aattcatggc gagaaaattt ttattaatac cggtgcacaa accgtggttc
     5641 cgccaattcc tggaattacc accacgcctg gtgtatatga cagcaccgga ttacttaatc
     5701 taaaagaatt gcctgggcat ttaggtattt tgggcggcgg atatattggc gttgagttcg
     5761 cctctatgtt cgctaatttt ggcagcaagg taaccatttt agaagcagct tcgctgtttt
     5821 tgcctcggga agatcgggat attgctgata atatcgcgac gattttacgc gatcagggcg
     5881 tcgatattat cctcaatgcc catgtggagc gaatcagtca caatgaaaat caagtgcaag
     5941 tgcatagcga gcacgcccaa ctggcggtgg atgcactgtt aatagcttcc ggtcgtcaac
     6001 cggctaccgc ttcgttacat ccagaaaatg ccggtatcgc agtaaacgag cgcggggcaa
     6061 ttgtcgttga caagcaatta cataccaccg cagacaatat ttgggcgatg ggagatgtta
     6121 ccggcgggct gcaatttact tatatttcac tggatgatta ccgcattgtg cgtgatgagt
     6181 tgctgggaga aggcagacgt agtactgatg atcggaaaaa tgtgccttat tccgtattca
     6241 tgacaccgcc actgtccagg gttggtatga cagaagaaca ggccagagag agtggtgctg
     6301 atattcaggt ggtgacattg cctgtagctg caattccgcg tgccagagtg atgaatgata
     6361 ctcgtggggt attaaaagcg attgttgata ataaaaccca acgtatatta ggggcatcac
     6421 tgctgtgtgt tgactcccac gagatgatca atatagtgaa aatggtgatg gatgccgggc
     6481 tgccttatag catattacgc gatcagatat ttactcatcc gtcgatgagc gaatcactca
     6541 atgatctatt ttcattagtc aaataaactc aaaatcagac gcaagaacaa atattctggc
     6601 gtctcagaga aaagaatctt attaattccc tgttactcta taactt
//
LOCUS       DAPGWD010000205         6452 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPGWD010000205 DAPGWD010000000
VERSION     DAPGWD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.207"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(51..347)
                     /locus_tag="RHX88_004072"
     CDS             complement(51..347)
                     /locus_tag="RHX88_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV2232691.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(561..1847)
                     /gene="thrC"
                     /locus_tag="RHX88_004073"
     CDS             complement(561..1847)
                     /gene="thrC"
                     /locus_tag="RHX88_004073"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDV2232692.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="RHX88_004074"
     CDS             complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="RHX88_004074"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDV2232693.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="RHX88_004075"
     CDS             complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="RHX88_004075"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDV2232694.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5270..5389)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5325..5390)
                     /gene="thrL"
                     /locus_tag="RHX88_004076"
     CDS             complement(5325..5390)
                     /gene="thrL"
                     /locus_tag="RHX88_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDV2232695.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5604..6290)
                     /locus_tag="RHX88_004077"
     CDS             complement(5604..6290)
                     /locus_tag="RHX88_004077"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV2232696.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         1543 a         1782 c         1566 g         1561 t
ORIGIN      
        1 cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat ttagcggtga
       61 tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg gtggcgcggc
      121 ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac
      181 cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc acgatcgcct
      241 atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc catgggagcg
      301 accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg ttactccttt
      361 tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga cggttgttat
      421 tctccgcaat cctaatctct aagttaccgc atttattgag aatttctcct ttttccctgt
      481 cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa acccggcctg
      541 attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa cgcagcaaaa
      601 tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag ctcttttggc
      661 agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc cggatgcgcg
      721 gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgatacgct
      781 acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat tgtctgttgc
      841 gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat tttgcggcgg
      901 aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc gttggataac
      961 gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg
     1021 ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgagtgactt cgccagcaga
     1081 cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg gttgcgcgct
     1141 tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag caaacggctg
     1201 atgttaatcg agttagctga gtttaacccc agcgccactt tcagttcttc atcatcaaac
     1261 gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata
     1321 ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag
     1381 aggataacca ctttcacatt cggtaaaccg tagaaagcat gagccactgc cgctccggta
     1441 tcaccggagg tcgcggtcag aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt
     1501 tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc
     1561 agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcgcgc
     1621 acgcgctctt ccaggatttc ctgcgggatt tcatcaccaa taaacgccga gaggatcttc
     1681 gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc
     1741 ggcaggtcat gcggaaaaaa cagcccctga tttttgccca acccctgggt tacggcttgc
     1801 gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta gttttccagt
     1861 actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg attttgcagg
     1921 tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg
     1981 aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc
     2041 tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc gataacatct
     2101 ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat gaagccagcc
     2161 agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa aatagccctg
     2221 gcttctgccg tcgagacttt aatccccgga tacgccagca cccacagcca ctcatcaaac
     2281 cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat accaccaaga
     2341 aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga ttcgtccttc cagctcgccc
     2401 atcaaagcca gcaaacgagt gtcattaagc ggcttgccac agtgttcatt catcgccatc
     2461 agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgattgg catattcttt
     2521 tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac
     2581 tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg tccgaggttg
     2641 ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa tgcaccatca
     2701 acaggtgtca ccgccgcccc gagcacatca aacccgacgc tcatattggc actggaagcc
     2761 ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac gtagcagatc
     2821 ggcaaagaca ccggcagctg taacgtcatt gcccgcacca tatccgcgca gcaccaacgg
     2881 cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt tcactttgaa
     2941 cagcggatca ttaccatcca cttcggcaat cttcacgcgg caggcaccat cttcatcaat
     3001 attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa
     3061 gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct cagcgttaaa
     3121 ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac gtcccgtttc
     3181 acgagcgaga atcaatagct tacgcgctac atccatacca gaaagatcat ctcgcggatc
     3241 cggttcggta taacccattt cccgcgccag agtagtcgcc tcggagaaac tcatgccttc
     3301 gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga acttcatcaa
     3361 ttcatcacca gcattgagca gattttgcag gttctcaata accggtaatc cagccccaac
     3421 gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcatgacgca acagatggta
     3481 gtaatccatc gacgaggtgt tggccttttt gttcggcgtg acaacgtgga aaccttcgcg
     3541 caagaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa caatgaccgg
     3601 gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa acggctcttt
     3661 ggcttgcgcc agttcttcct gccagttttc cagatttagg ccatgcacat tggtgagcag
     3721 tgccttcgag ttggcaacac cgcagacacg taagtcgata tgtttattct tcagccagct
     3781 ttgttgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc caatcacaaa
     3841 cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca cgccagtggt
     3901 cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct gagcaatggc
     3961 gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa
     4021 ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca ccgccagcgg
     4081 ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc gttcagcccg
     4141 cacacagtcg ctttgcggaa cgcagaaact gatactgtat tcggaagatg attgcgtaat
     4201 cagcaccacg gaaatacggg cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac
     4261 cattcctttc atccccgggc cggaaacgct gaacattgcc atgttattca gattggaaat
     4321 gcccttgacc ggtaattcgt cttcatcacg actggcacca atgagcgtac ctggtgcttg
     4381 aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg gggtaatggt
     4441 gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct ggtaggacat
     4501 cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga ccccgtcaac
     4561 gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg ccgcggagta
     4621 gtcggaaccg ttgcgtccaa gtaccaccag ttcgcctttt tcattaccgg cggtgaaacc
     4681 tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc gggtggactc
     4741 agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt cgaccggatc
     4801 gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg cgatcgacat
     4861 tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc ccaacaaact
     4921 aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag ttttcaattg
     4981 cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg
     5041 ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt caatcatcgc
     5101 caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct gcccctgcct
     5161 ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg ccactgatgt
     5221 accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc
     5281 ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta
     5341 cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg
     5401 tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac
     5461 ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag ctgctatcag
     5521 acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg
     5581 aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa
     5641 cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgttg taaccagtcg
     5701 accagtttta tgtcatctgc cactgccaga gtcgtcagca atgccatgac tcgttcgcgt
     5761 aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat
     5821 gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc
     5881 gccaccatcg gcacaccagt aagaacgtca gccaacgcta actcttcgtt agtcagcccg
     5941 gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac
     6001 agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact gcgcgcagtg
     6061 gtggcgacag tgaaatcgac atcgtgtaac gattcaacca gtgtcgggaa aactttaata
     6121 ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc cagatgtgcc
     6181 tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc
     6241 ccaatatttt ctgctctggc gggtgcgacc agaataatcg ttatacgcat attgccactc
     6301 ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc ggtgcgaatt
     6361 tacaaattct taacgtaagt cgcagaaaaa gccctttact tagcttaaaa aaggctaaac
     6421 tatttcctga ctgtactaac ggttgaattg tt
//
LOCUS       DAPGWD010000206         6423 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPGWD010000206 DAPGWD010000000
VERSION     DAPGWD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.208"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            33..998
                     /gene="iaaA"
                     /locus_tag="RHX88_004078"
     CDS             33..998
                     /gene="iaaA"
                     /locus_tag="RHX88_004078"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV2232697.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            985..2856
                     /gene="gsiA"
                     /locus_tag="RHX88_004079"
     CDS             985..2856
                     /gene="gsiA"
                     /locus_tag="RHX88_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDV2232698.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            2876..4414
                     /gene="gsiB"
                     /locus_tag="RHX88_004080"
     CDS             2876..4414
                     /gene="gsiB"
                     /locus_tag="RHX88_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDV2232699.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            4432..5352
                     /gene="gsiC"
                     /locus_tag="RHX88_004081"
     CDS             4432..5352
                     /gene="gsiC"
                     /locus_tag="RHX88_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDV2232700.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            5355..6266
                     /gene="gsiD"
                     /locus_tag="RHX88_004082"
     CDS             5355..6266
                     /gene="gsiD"
                     /locus_tag="RHX88_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDV2232701.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT         1411 a         1551 c         1890 g         1571 t
ORIGIN      
        1 aagtggcgag ccacccttaa tggacgaata ctatgggcaa agcagtcatt gcaattcatg
       61 gtggcgcagg tgcaattagc cgcgcgcaga tgagtctgca acaggaatta cgctacattg
      121 aggcgttgtc tgccattgtt gaaaccgggc agaaaatgct ggaagcgggc gaaagtgcgc
      181 tggatgtggt gacggaagcg gtgcgtctgc tggaagagtg tccactgttt aacgccggaa
      241 ttggcgctgt ctttacgcgt gatgaaaccc atgaactgga cgcctgtgtg atggatggta
      301 acaccctgaa agccggtgcg gtggcgggcg ttagtcatct gcgtaatccg gttcttgccg
      361 cccggctggt gatggagcaa agcccgcatg tgatgatgat tggcgaaggg gcagaaaatt
      421 ttgcgtttgc tcgtggcatg gagcgcgtct cgccggagat tttctccacg cctttgcgtt
      481 atgaacaact actggcagcg cgcaaggaag gggcaaccgt cctcgaccat agcggtgcgc
      541 cactggatga aaaacagaaa atgggcaccg tgggggccgt ggcgttggat ttagacggca
      601 atttggcggc agccacgtcc acaggcggaa tgaccaataa attacccgga cgagttggcg
      661 atagtccctt agtgggtgcc ggatgctacg ccaataacgc cagtgtggcg gtttcttgta
      721 ccggcacggg cgaagtcttc atccgcgcgc tggcggcata tgacatcgcc gcgttaatgg
      781 attacggcgg attaagtctc gcggaagcct gcgagcgggt agtaatggaa aaactccctg
      841 cgcttggcgg tagcggtggc ttaatcgcta tcgaccatga agggaatgtc gcgctaccgt
      901 ttaacaccga aggaatgtat cgcgcctggg gctacgcagg cgatacgcca accaccggta
      961 tctaccgtga aaaaggggac accgttgcca cacagtgatg aacttgatgc cggtaatgtg
     1021 ctggcggttg aaaatctgaa tattgccttt atgcaggacc agcagaaaat agctgcggtc
     1081 cgcaatctct cttttagtct gcaacgcggt gagacgctgg caattgttgg cgaatccggc
     1141 tccggtaagt cagtgactgc gttggcattg atgcgcctgt tggaacaggc gggcggttta
     1201 gttcagtgcg ataaaatgct gttgcagcgg cgcagtcgcg aagtgattga acttagcgag
     1261 cagagcgctg cacaaatgcg ccatgttcgc ggtgcggata tggcgatgat atttcaggag
     1321 ccgatgacat cgctgaaccc ggtatttact gtgggtgaac agattgccga atcaattcgt
     1381 ctgcatcaga acgccagtcg tgaagaagcg atggtcgagg cgaagcggat gctggatcag
     1441 gtacgcattc ctgaggcaca aaccattctt tcacgttatc cgcatcaact ctctggcggg
     1501 atgcgccagc gagtgatgat tgcgatggcg ctgtcatgcc gcccggcggt gctgattgcc
     1561 gatgagccaa ccaccgcgct ggatgtcact attcaggcgc agatcctgca attaatcaaa
     1621 gtattgcaaa aagagatgtc gatgggcgtt atctttatca ctcacgatat gggcgtggtg
     1681 gcagagattg ccgatcgggt actggtgatg tatcagggcg aggcggtgga aacgggtacc
     1741 gtcgaacaga tttttcatgc accgcaacat ccttacaccc gtgcgctgtt agctgctgtt
     1801 ccgcaacttg gtgcgatgaa agggttagat tatccccgac gtttcccgtt gatatcgctt
     1861 gaacatccag cgaaacaggc cccccccatc gagcagaaaa cggtggtgga tggcgaacct
     1921 gttttacgag tgcgtaatct tgtcacccgt ttccctttgc gcagcggttt gttgaatcgc
     1981 gtaacgcggg aagtgcatgc cgttgagaaa gtcagttttg atctctggcc tggcgaaacg
     2041 ctatcgctgg tgggcgagtc tggcagcggt aaatccacta ccgggcgggc gttgctgcgc
     2101 ctggtcgaat cgcagggcgg cgaaattatc tttaacggtc agagaatcga taccttgtca
     2161 cccggcaaac ttcaggcatt gcgccgggat attcagttta tttttcagga cccttacgct
     2221 tcgctggacc cacgtcagac catcggtgat tcgattatcg aaccgctgcg tgtacacggt
     2281 ttattgccag gtaaagaagc ggctgcacgc gttgcgtggt tgctggagcg cgtgggcctg
     2341 ttacctgaac atgcctggcg ttacccgcat gagttttccg gcggtcagcg ccagcgcatc
     2401 tgcattgctc gcgcgttggc attgaatccg aaagtgatca ttgccgacga agccgtctcg
     2461 gcgctggatg tttctattcg cgggcagatt atcaacttgt tgctcgatct ccagcgtgat
     2521 ttcggcattg cgtatctgtt tatctcccac gatatggccg tggtagagcg gattagtcat
     2581 cgtgtggcgg tgatgtatct cgggcaaatt gttgaaattg gtccacggcg cgcggtcttc
     2641 gaaaacccgc agcatcctta tacgcgtaaa ttactggcgg cagttccggt cgctgaaccg
     2701 tcccgacaac gaccgcagcg tgtactgctg tcggacgatc ttcccagcaa tattcatctg
     2761 cgtggcgaag aggtggcagc cgtctcgttg caatgcgtcg ggccggggca ttacgtcgca
     2821 caaccacaat cagaatacgc attcatgcgt agataacatt caggcggaga ataaaatggc
     2881 aagagctgta caccgtagtg ggttagtggc gctgggcatt gcgacagcgt tgatggcatc
     2941 ttgtgcattc gctgccaaag atgtggtggt ggcggtagga tcgaatttca ccacgctcga
     3001 tccgtatgac gcgaatgaca cgttatctca ggccgtagcg aaatcgtttt accaggggct
     3061 gttcggtctg gataaagaga tgaaactgaa aaacgtgctg gcggagagtt ataccgtttc
     3121 cgatgacggc attacttaca ccgtgaaatt gcgggaaggc attaaattcc aggatggcac
     3181 cgatttcaac gccgcggcgg tgaaagcgaa tctggaccgg gccagcgatc cggcgaatca
     3241 tcttaaacgc tataacctgt ataagaatat tgccaaaacg gaggcgatcg atccaacaac
     3301 agtaaagatt accctcaaac agccgttctc agcgtttatt aatattcttg cccatccggc
     3361 gaccgcgatg atttcaccgg cagcgctgga aaaatatggc aaggagattg gttttcatcc
     3421 ggtgggaacc ggaccgtatg aactggatac ctggaatcag accgattttg tgaaggtgaa
     3481 aaaattcgcg ggttactggc agccaggatt gcccaaactg gacagcataa cctggcgtcc
     3541 ggtggcggat aacaacaccc gcgcggcaat gctgcaaacc ggtgaagcgc agtttgcttt
     3601 ccccattccc tacgagcagg ccacactgct ggagaaaaac aaaaatatcg agttgatggc
     3661 cagtccgtca attatgcagc gttatatcag tatgaacgtg acgcaaaagc cgttcgataa
     3721 cccgaaggtc cgtgaggcgc tgaattacgc cattaaccgt ccggcgctgg tgaaagttgc
     3781 ctttgcgggc tatgcaacgc cagctactgg tgtggtaccg ccaagtatcg cctacgcgca
     3841 aagttataaa ccgtggcctt acgatccagt gaaagcgcgc gaattactga aagaggcggg
     3901 atatcccaac ggtttcagta ccacgctgtg gtcgtcacat aaccacagca ccgcgcagaa
     3961 agtgctgcaa tttacccagc agcagttagc gcaggtcggg attaaagccc aggtgaccgc
     4021 gatggatgcc ggacagcggg cggcagaagt tgaaggtaaa gggcaaaaag agagcggcgt
     4081 gcggatgttc tacactggct ggtcggcttc aaccggcgaa gcggactggg cgctatcgcc
     4141 actgttcgcc tcgcaaaact ggccaccgac gctgtttaat accgcatttt acagcaataa
     4201 acaggtggac gacttcctgg ctcaggcact gaaaactaat gatccggcgg aaaagacccg
     4261 cttgtataag gcggcgcagg atatcatctg gcaagagtcg ccgtggatcc cgctggtggt
     4321 agaaaaactg gtgtcggcac acagtaaaaa cctgaccggt ttttggatca tgccagacac
     4381 cggcttcagc tttgaagacg cggatttgca ataagcaacg cagggagtgg aatgcttaat
     4441 tacgttatca aacgcttact ggggttgatt ccgacgctgt ttatcgtctc ggtgctggtg
     4501 tttttatttg tgcatatgct gcccggcgat ccggcgcgat tgattgccgg gcccgaagct
     4561 gatgcgcagg ttatagaact ggtgcgtcag cagctggggt tggatcagcc gctgtatcac
     4621 cagttctggc actatatcag caatgctgtg cagggggatt ttggcctgtc gatggtgtcg
     4681 cgtcgtccgg ttgccgatga gattgccagc cgctttatgc caacgctgtg gctgaccata
     4741 accagtatgg tctgggcggt gatatttggt atggcggcgg gaattatcgc cgccgtctgg
     4801 cgtaatcgtt ggccggatcg attgagtatg accattgcgg tgtcggggat ctcgtttccg
     4861 gcatttgcgc tggggatgct tttaattcag gtattctccg ttgaactggg ctggctgcct
     4921 accgtgggag cagacagttg gcagcactac attttaccct ccctgacgct cggcgcggca
     4981 gtggcagccg tgatggcgcg ctttacccgc gcgtcgtttg tcgatgtttt aagcgaagat
     5041 tatatgcgta ccgcgagggc gaaaggggtg agcgaaacct gggttgtcct caaacacggg
     5101 ctacgtaacg cgatgatccc ggtagtgacc atgatgggct tacagtttgg ctttttgctc
     5161 ggtggttcta tcgtggtgga gaaagttttc aactggcccg gacttggacg cttactcgtt
     5221 gactccgtag aaatgcgcga ttacccggtg attcaggcgg aaattctgct tttctcgctg
     5281 gaatttattc ttatcaactt agtggtggat gtgctttacg ccgccattaa cccggctatc
     5341 aggtacaagt aaggatgcga ctatttaact ggcgacgtca ggcggtgtta aacgccatgc
     5401 cactggtcaa acctgaccag gtgcgtacac cgtggcatga attctggcga cgatttcgcc
     5461 gtcagcatat ggcgatgacc gccgcattat tcgttatttt attgattgtg gtggccattt
     5521 ttgcacgctg gatcgctccc tatgacgccg aaaattattt tgattatgac aatctgaata
     5581 acggaccttc tttgcagcac tggtttggcg tcgattcact ggggcgcgac attttcagcc
     5641 gtgtcctggt tggtgcgcaa atctcgctgg cggcgggcgt gtttgccgtg tttatcggtg
     5701 cggcgatcgg gacgttgctg ggcttgttgg ctggatatta tgaaggctgg tgggatcggt
     5761 tgatcatgcg catttgcgat gtgctgtttg ccttcccggg tattttactg gcgatcgctg
     5821 ttgttgcggt gttgggaagc ggcattgcta acgtgattat cgcagtcgcc attttttcca
     5881 tccccgcgtt tgcccgcctg gtgcgcggca acacgctggt gttgaaacag caaaccttta
     5941 ttgagtcagc acgcagtatt ggtgccagcg atatgaccat tttattgcgt catatcttgc
     6001 ctgggaccgt ctcgtctatc gtggtgtttt tcaccatgcg cattggcacc tcgattatct
     6061 ctgccgccag cctgtcattt ctcggcctcg gtgcgcagcc gccgacacca gagtggggag
     6121 caatgctcaa tgaggcacga gcggatatgg ttatcgcgcc gcatgtcgct gtttttccgg
     6181 ccctggctat ttttctgacc gtactggcgt tcaatttgtt gggcgatggt ttacgcgatg
     6241 cgctggatcc gaaaattaaa ggatagttac gtttgaatat tgcttgaaag ggtaatcacc
     6301 tcacaggcaa ttattgccct aagcaagtgt tgtaactttc tgctgatttt gtagaatcgg
     6361 gtaatttggt taaaaagccg cagcaaggga ctatttttgc agcggcacag cgttcagata
     6421 gtt
//
LOCUS       DAPGWD010000207         6421 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPGWD010000207 DAPGWD010000000
VERSION     DAPGWD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.209"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..993
                     /gene="tssI"
                     /locus_tag="RHX88_004083"
     CDS             <1..993
                     /gene="tssI"
                     /locus_tag="RHX88_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV2232702.1"
                     /translation="LTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGTALENHFAV
                     IPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNPADQDSS
                     CWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGT
                     KTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETV
                     KNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGD
                     GIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
     gene            1021..1482
                     /locus_tag="RHX88_004084"
     CDS             1021..1482
                     /locus_tag="RHX88_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HDV2232703.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1502..6019
                     /locus_tag="RHX88_004085"
     CDS             1502..6019
                     /locus_tag="RHX88_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HDV2232704.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRLCA
                     GTSLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLARLGKLGNLLGKSGKLLGAKLGALLSRTKNALKNTYNVLKKFIK
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHCSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GKRVDIISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVTVTGAEGYYNATLDYGDGCTTLTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWQ
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     IERAGTGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVISPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCIQPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVFRHSNRGFACEQGWNAASQLVTQRAGFFPEETTWGGLLPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSR
                     DQLTGYCSAQGELWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRERQAHPSVAPLWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGEWDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLLGGEQTYRYVPNPLRWIDPLGLNKGASLSKMMNSSSDLMGLRRQPQ
                     NFWRLYRGKDI"
     gene            6052..6312
                     /locus_tag="RHX88_004086"
     CDS             6052..6312
                     /locus_tag="RHX88_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232705.1"
                     /translation="MLEHFGAEASVLDMTIIVRSNPSKAAILEEFLHGTQEKLGIAEK
                     LGRYGLGSAETHVKDFMIRHKKMLGLSDEDVAILKILKDKGL"
BASE COUNT         1581 a         1725 c         1960 g         1155 t
ORIGIN      
        1 ctgacggggc atccgcaggc gaacctgaac cgggaatggc aggtggtggc aagtgaactg
       61 cacggcgaac agccacaggc ggtgccagga cggcagggag cggggacggc gctggagaac
      121 cattttgcgg tgatcccggc agacagaaca tggcgaccac agccgttgct gaaaccgctg
      181 gtcgacggcc cgcagagcgc tgtcgtgaca ggaccggcag gcgaggaaat cttctgcgac
      241 gaacatggtc gcgtgcgggt gaagttcaac tgggaccgtt ataacccggc agaccaagac
      301 agttcgtgct ggatccgtgt ggcacaggcg tgggcaggca ccggttttgg ccacctggcg
      361 ataccgcgtg tgggtcagga ggtgattgta gacttcctca acggcgatcc ggaccagccg
      421 atcattatgg ggcgcaccta ccaccaggaa aaccgcaccc ccggcagcct gccgggaaca
      481 aagacgcaga tgaccatccg ctccaaaacg tacatgggca gcggatttaa tgagctgaag
      541 tttgatgatg cgacagggag agaacaggtc tacatccacg cgcagaagaa catggatacc
      601 gaagtgctca acgaccgtac caccaccgta aaacacgatc atcgcgaaac cgtaaaaaat
      661 gaccagacgg tcacgatcca ggaaggtaac cgccttctta cggtggaaaa aggccacaaa
      721 atcaccggag tactgaaagg gtctttatct gaggatgtct ttcaggacag aggcacgatt
      781 gccggttcgg tgcatgttga cgctgtaaac aatggtggcg aaggcgacgg tatacaggct
      841 tatacggcga ttaaggaaat tttgctggcc gtggaggaaa gcaaaattgc gctgacgccg
      901 gatggcattc agctacaggt cggcgaatcg acggtaatca ggctgtcgaa ggatggcatc
      961 accatcgtgg gcggttctgt tttcatcaac tgattcactc agattatcag ggagtatttt
     1021 atgcagttca cttttaatga aggccacatt caactgccgt ctcaatggca ggatcagtca
     1081 atgcaggtcc tggtgtccac agataacagt ggcatcaacc tggtcattac ccgcgaagcc
     1141 gttccacagg gaaccctgac accagagctg taccaggaaa cgctggcgct gtaccagggc
     1201 aaacttgatg ggtacaccga acatgcctgc cgggaaatca ctctggcaga ggccccggcc
     1261 tggctgctgg attattcctg gaacggtccg gaagatgagg ggaatcaggg gcggattagc
     1321 cagatagccg tattccagcg gcgtggagat acgctgctga ccttcacttt cagcacctcc
     1381 ttatccctga agaacagtca aaagacgatg ctgctggagg tgataaagag cttcaccccg
     1441 ttgccaccgg agaatgacat ccaaaaggac cagccgcgct gacataaacg agggataaca
     1501 catgagtgaa ggaccaggcg ggccacaggg agcgaccgca ggcggtacgc tggcaatgcg
     1561 aatgctgtca cagcaggcga tggtcgccag ccagatgaaa cgggcagcca acgacaaagc
     1621 cattgcacag atgctggcag caaagaagtc cggcccacct gccgccaggc tgggcgatga
     1681 aattcagcat aaaagttttc tgggggcact ggcaggggcc gtgctggggg cgatagtgac
     1741 catcgcagaa ggttgcctga ttatggccgc ctgtgccacc ggcccttatg cgctggttct
     1801 ggtgcctgcg ctgatgtatg ccagctataa ggcgagtgat tatgtggagg agaaacagaa
     1861 ccagcttgag tcatggataa acagcttttg tgacacggac ggcgccatca ataccggttc
     1921 tgaaaatgta aacattaacg gaaagcccgc tgccagagcc gccgtcaccc ttccccctcc
     1981 tcccccacct ggagcaatac ctgaagtccc acagggggaa ccctcatggg gtgatattgc
     2041 cactgacctg cttgaatcgg cagcggaaaa agcagtacca ctggcgaagg cctgggggaa
     2101 cgctgttatc accctgacgg acagcaatgc cggttttatg gatcgcttat gtgcaggcac
     2161 atcgcttctg tttcccgccg gtccggtatt aatggagttt gccaccatgg tgggcgggcg
     2221 tggcgaaatc aaaaaagacg tggatttccc ggaagccggt gaggacacgg cgctctgcga
     2281 caaggagaac aaaccaccga ggatagccca gggcagcagc aacgtcttta tcaacaatca
     2341 gcctgccgcg cgcaagggcg acaaactgga gtgcagcgcg gcaatcgtgg aaggttcgcc
     2401 ggacgtcttt attgggggtg agcaggtcac ctatctggat atccagccgg agttcccgcc
     2461 atggcagaga atgatcctgg gaggaataac gatagccagc tatcttctgc cgccagcagg
     2521 actgctggga aaactgggga atctggcgag actcggcaaa ctgggaaacc tgctggggaa
     2581 aagcgggaag ctgctgggtg caaaactcgg cgcattgctg agcagaacaa aaaatgccct
     2641 gaaaaatacg tataacgtcc tgaaaaagtt tatcaaagat ccggttgacc cggtaaccgg
     2701 cgcgtactgc gacgaacgta ctgacttcac cctgggccag acactccccc tctccttcac
     2761 ccgcttccac tgttcggtac tgccactgca tggcctgacc ggcgtgggct ggagcgattc
     2821 ctggagcgaa tacgcctggg tgcgtgaaca gggaaagcgg gtggatatca tcagcctggg
     2881 agccacgctg aacttcgcct tcgacggtga aagtgatacg gcggttaacc cgtatcacgc
     2941 ccagtacatc ctgcgccgcc gtgatgatta cctggagctg ttcgaccgtg atgccctgag
     3001 cagccgcttc ttttatgacg cctttccggg aatgcgcctg cgccacccgg tgactgacga
     3061 taccagcgat gaccgcctgg cacacagccc cgcagaccgg atgtacatgc tgggcgggat
     3121 gagcgacacc gccagcaacc gcatcacgtt tgagcgcgac agccagtacc ggatcacagg
     3181 tgtcagtcac accgacggga tccggcttaa actgacgtac catgccagcg gctacctgaa
     3241 agccattcac cgtacggata acggcataca gacgctggcg acctacgaac aggatgcgcg
     3301 ggggcggctg acagaagcgg atgcgcggct ggactaccac ctgttttatg agtacgacgc
     3361 tgcggaccgg atcatccgct ggtccgataa cgaccagacg tggagccgtt tcacctacga
     3421 tgaacagggc cggtgcgtga ccgtcaccgg ggcggagggc tattacaacg ccacgctgga
     3481 ctatggtgac ggctgcacca ccctgacgga cggcaagggc actcaccgtt attactatga
     3541 tcctgacggc aatattctgc gggaagaagc gccggacggc agcaccacca cgtatgaatg
     3601 ggatgaattc catcacctgc tggcccgcca ctcccctgcc gggcgggtgg agaaatttga
     3661 atacaacgcc gcacacggcc agctaagccg ttacacggca gcagacggtg cggagtggca
     3721 gtaccgctat gatgagcgcg gcctgctcag caacatcacc gaccctgccg ggcagacgtg
     3781 gacacagcag tgtgatgaac gcggcctgcc ggtaagcctg gtgtcgccac agggcgaaga
     3841 gacccggctg gcatacaccg ctcaggggct gctgtcgggg atattccgcc aggatgaacg
     3901 gcgtctgggc atagagtacg accaccacaa ccggccggaa acactcaccg acgtgatggg
     3961 ccgtgaacac cacaccgaat acagtggtca cgacctgccg gtgaagatgc gcggccccgg
     4021 cggtcagtca gtgcggttac agtggcagca gcaccataaa ctgagcggca ttgaacgtgc
     4081 tggaaccggc gcggaaggat tccgctatga ccgccacggc aacctgctgg cgtacacgga
     4141 cggtaacggc gttgtctgga caatggaata cggcccgttc gatttgccgg tggcgcgaac
     4201 ggacggtgaa ggccaccgct ggcagtaccg ctacgataaa gacacgctgc agctcacaga
     4261 agtcattaac ccgcagggcg agtcataccg ttatattctg gacaactgtg gccgggtgac
     4321 ggaagagcgt gactggggcg gcgtggtctg gcgttaccgc tatgacgctg atggcctgtg
     4381 taccgccagg gtcaacggcc tggaggaaac catcctctac agccgggacg ccgcaggccg
     4441 cctggcagaa gtcatctcgc cggaagggaa aacacagtat gcctatgaca aatccggcag
     4501 gctgacgggt atcttcagcc cggacggcac atcacagcgc accggctacg acgaacgcgg
     4561 gcgggtgaat gtcaccactc agggccgacg ggccattgaa taccactacc ccgatgaaca
     4621 caccgttatc cgctgtatcc agccaccgga agatgaacgc gacagacacc ccgatgaatc
     4681 cctgctgaaa accacgtacc gttataacgc cgccggagaa ctgacggagg tcattctgcc
     4741 gggggatgag acgctgacgt tcagccgtga tgaggcggga cgtgaagtgt tccggcacag
     4801 taaccggggt tttgcctgtg agcagggctg gaatgcagcc agccagcttg tcacccagcg
     4861 cgccggattt ttcccggagg aaaccacatg gggcgggctg ctcccctcac tggtacggga
     4921 gtaccgttac gacagcgcgg gtaacgtgtc aggcgtcacc agccgggaag attacggacg
     4981 ggaaacacgg cgggaatacc ggctggaccg gaacggtcag gtcacggcgg tgacagcctc
     5041 aggcaccggg ctgggctatg gcgaaggcga tgagtcctat ggctatgaca gttgtggcta
     5101 cctgaaggcg cagtctgcgg gcaggcaccg gataagtgaa gagactgacc agtatgccgg
     5161 aggccaccgg ctgaaacagg ccggaaacac gcagtatgac tatgacgccg caggccggat
     5221 ggtcagccgg acaaaacacc gtgacggcta ccgcccggaa acagagcggt tccggtggga
     5281 cagccgggac cagctgaccg ggtattgcag cgcacagggg gagctgtggg aataccgcca
     5341 cgacgccagc ggcagacgaa cagagaaacg ctgcgaccgg aagaaaatcc gcttcacgta
     5401 cctgtgggac ggcgacagta ttgcggaaat ccgggaatac cgcgatgata aactgtacag
     5461 cgtacggcac ctggtgttta acggctttga gctgataagc cagcagttca gccgggaacg
     5521 gcaggcgcat ccgtccgtgg ccccgctgtg ggtgacgcgg acgaatcatg cggtgagcga
     5581 cctgacgggc cgcccgctga tgctctttaa cagtgaaggt aaaaccgtct ggcggccggg
     5641 acagaccagc ctgtgggggc tggcactcag cctgcccgca gacaccggct acccggaccc
     5701 gcgcggggag tgggacccgg aagccgaccc cggcctgctg tatgcgggac agtggcagga
     5761 tgcggaatcc gggctgtgct ataaccggtt ccggtattac gagccggaaa ccggaatgta
     5821 cctggtgagt gatccgctgg ggttgctggg cggggagcag acttaccggt atgtgccgaa
     5881 tcctttaaga tggatagatc ccttaggatt aaataaagga gcttcattat ctaaaatgat
     5941 gaatagctcc agtgatctca tggggttgag aaggcagccc cagaacttct ggcggctata
     6001 tcgcggaaaa gacatttaat aatagcatta ccaggctcag atgaattcag aatgcttgaa
     6061 cattttggag ccgaagcttc ggtattagac atgacgatta ttgttcgttc aaatccgagt
     6121 aaagccgcta ttcttgaaga gtttttacat ggtacacaag agaaactagg aatagctgaa
     6181 aaattaggac gttatggact agggagtgca gaaacgcacg ttaaggattt catgataaga
     6241 cacaaaaaaa tgcttgggtt atctgatgag gatgtagcaa tactcaaaat attgaaagac
     6301 aaggggttgt gatgaatata attctgccga taaaagatat atttattatt gcgggaaaca
     6361 tagtttttag cgttttatac aatgaacatc ttgagttccc atgccgctgc caattactat
     6421 c
//
LOCUS       DAPGWD010000208         6380 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPGWD010000208 DAPGWD010000000
VERSION     DAPGWD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.210"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..365
                     /locus_tag="RHX88_004087"
                     /pseudo
     CDS             1..365
                     /locus_tag="RHX88_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(369..1760)
                     /gene="tcyP"
                     /locus_tag="RHX88_004088"
     CDS             complement(369..1760)
                     /gene="tcyP"
                     /locus_tag="RHX88_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDV2232706.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFATFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1893..2483)
                     /locus_tag="RHX88_004089"
     CDS             complement(1893..2483)
                     /locus_tag="RHX88_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2232707.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2646..3314)
                     /gene="hxpB"
                     /locus_tag="RHX88_004090"
     CDS             complement(2646..3314)
                     /gene="hxpB"
                     /locus_tag="RHX88_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDV2232708.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3461..3997
                     /locus_tag="RHX88_004091"
     CDS             3461..3997
                     /locus_tag="RHX88_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDV2232709.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4038..4898)
                     /locus_tag="RHX88_004092"
     CDS             complement(4038..4898)
                     /locus_tag="RHX88_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDV2232710.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5004..5294)
                     /gene="ghoS"
                     /locus_tag="RHX88_004093"
     CDS             complement(5004..5294)
                     /gene="ghoS"
                     /locus_tag="RHX88_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV2232711.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5395..6324)
                     /gene="pfkB"
                     /locus_tag="RHX88_004094"
     CDS             complement(5395..6324)
                     /gene="pfkB"
                     /locus_tag="RHX88_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDV2232712.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1652 a         1591 c         1562 g         1575 t
ORIGIN      
        1 tatacatcct gccgggagca tttttgctgc gattcaaaaa aaatgagagt ctcctaaagt
       61 ttccttcgat aatatcaatg cttaatatca atcttctttt taatccacta aatcttccgg
      121 gaatgggaag tggtgtgctt gaagatatta tgagtattcc agatagttca cttagaaaac
      181 gtctcgggta tgaggtttta agcttctcac tacaggcaca ttcattgagc caggaatgta
      241 ttgataagtt ggacattttt ttcgctgacg atctgtttaa atatgagtca gtatgcatcg
      301 ccgcgatgga acatttaaaa agtaaagcaa cggcccctat acaaaacgga ccgttgccag
      361 cataagaatt aacggtgtgc cagttcggcg tcgtcttcgc tatccagaat ggctttatcg
      421 gtttgcttca gccactggct ggtcagcgtg ccagctgtca tcgagccact aacgtttagc
      481 gccgtacggc ccatgtcgat aagcggttca acggagatta acagcgccac cagggttact
      541 ggcaggccca tcgcaggcag cacaatcagt gcggcgaaag ttgcaccacc accgacaccg
      601 gcaacgcctg cggaactaac ggtaacaata ccgaccagcg tcgcaatcca catcgggtcc
      661 agcgggttaa tgccaaccgt aggcgcaacc atcaccgcca gcattgccgg atacaaaccg
      721 gcgcagccgt tctgaccaat ggttgcaccg aaagaggcgg cgaaactggc gatggattca
      781 ggaacgccca gacgacgcgt ttgtgcttcc acattcagtg ggatagacgc agcactggaa
      841 cggctggtaa aggcaaacgt cagcacaggc cataccttac ggaagtactt cagcggactc
      901 acgccattaa tgcccagcag aatgccatgc actgcaaaca taatcagcag accgaggtag
      961 gacgcgacaa cgaaacttcc cagtttgatg atgtcttgca agttagaacc tgcaaccact
     1021 ttggtcatta gagccagaac gccgtaaggg gtcaactgca tgacaaggcg aaccagtttc
     1081 atcacccagc tttgtagggt atcgatagcg gctaagacgc gttcaccttt cggcgcatca
     1141 tccttcagca gtttcaatgc agctacgccg aggaatgtgg caaaaattac cacgctgata
     1201 attgacgtcg gattggctcc ggtaagatcg gcaaacgggt ttttcgggat aaaggacaag
     1261 accagctgcg gaacgctcag atcagagact ttaccaacat agttactttc aatggcgttc
     1321 agacgtgcag tttctgcacc accctgaacc agaccttcag ccgtcaaacc aaacaggttg
     1381 gtgaccagca caccgaccag cgccgcaatc agcgtggtaa acaaaagcgt accgatggtc
     1441 agaaaactga ttttgcctaa ctgagatgcg ttatgcagac gggcaactgc gctcagaata
     1501 gaggcgaaga ctaacggcat aacgatcatt tgcagcagtt gaacatagcc gttaccaacg
     1561 atgttaaacc actgtacaga atctttaagt acctggctgt cagaaccata aatggtatgc
     1621 agggcaaggc caaaaaccac acccatcacc agacccacca gcactttttt cgccagactc
     1681 cactgtttat ggcgggtctg agccagcgca aacagcagta caacgaacac cacgatgttc
     1741 gcaattaatg gaaagttcat ccccgttctc ctgatcttat tatttctatc actgcgtagc
     1801 agtcatagtg ttggcgcaag attaacagaa ctgtgagctg gctcttatat tcaaatggaa
     1861 ttgcttattc cagaaaggtg atttttgcct ttttagtatt caacctgatg cttaataagc
     1921 cgatgaaact ggatctgcaa ggtattgatc atttgcgatg accaacgaac cgcgctgttt
     1981 tcgggtaatg aatggggcat ccataccgac cagacaaata atgccatgca gataaaacgt
     2041 tccagttggt tgcccttttg gggaaccagg ataggcaagc ggaaacgcca gcgacatggc
     2101 cagagcaggg gaacaccggc gggtgtcagc atatcggcaa gtatgtggct taaataaccc
     2161 agcaccattc cttgtagcgc atcagccgga atgaaccagc tttcaggaac cttaaggtaa
     2221 aaggttgcca gcagcgcaaa taccgccagc agactgtggg taaaaccacg atgcccaaaa
     2281 gcgcgggcga tcggttttga tatccatttt aatcgctgcc caagaaacga ctttgggtga
     2341 tcgatgtccg gtaacaaaca cgtcaggatt gcggaaggga caatatgcca ccagtcaccc
     2401 tgtgccagca cgggcgtcag ctcggcattt ttggcaaata ccgcacaagc aatagaaaag
     2461 agaaggtgac cttccgccgt catgataaaa ctcactaaac tgtcgatacg tacagtatag
     2521 gatttttata cagtgcacgg aagtggtgac ggtgtaactc tttctcttaa attgacgatt
     2581 aatccgtcag ttgaatgcaa attcagataa agaaccaggg gcagcgagat cactgcccct
     2641 ggtcattaac cgagaaggtc ttttgcggtg agttctgtca gcgatgagag tttgacgttt
     2701 gctaatacaa aacgtggatc attttgcgct tctggcgcag gaacgacaat ggaacgcatg
     2761 cgggctgctt tagaggcgat catgccattt actgaatctt ccagcgctac gcaggtcagt
     2821 gggtcaacgc ccagttttgc tgcgcagtcg agatatactt gcggatgcgg cttgctgtaa
     2881 ggcagttttt cagccgaggc gagggcatcg aaactgtcgc gtaagtcaaa catggtcaac
     2941 actttttcca gcatatgtag tggtgacgcg gaggccagtc ccaccaataa accttgttct
     3001 ttgcataacg caacggcttc gcgcacgcct ggtaataatg gacgtgtctc ttcaaccagt
     3061 gaaatggcac gggcaataac ccgttctact acttcctgac ggcttggccc attccacggt
     3121 tgccgggcgt accaaagatc gaccaccata tcgatgcgta aacctaaggt gtccggcagc
     3181 tcgttacgac gggagatatc cacccccagg cttgccatca catccagttc ggctcgatcc
     3241 cataaaggtt ctgagtcgat aagtaatcca tccatatcaa aaattgcagc aagaatctga
     3301 cgcggggttg acattacaac ctctccttta gagcattatg tgtaaggtat taagccgtgc
     3361 ttatgattaa gcgcctatca tatcgccttt aaagaatggg cgaaaacaga tatctacagg
     3421 taaactaagt gaaacgacgc gtcttaatca aggagaactc atgacgtatc aacaagctgg
     3481 acgcattgct gttttgaaaa ggattttggg ttgggtgatt tttatacctg cattgatctc
     3541 tacattgatt tctttgctga aatttatgaa tacccggcag gaaaaccagg aaggcattaa
     3601 tgcggtcatg ctcgacttta ctcatgtcat gatcgatatg atgcaggcga atacgccttt
     3661 tttgaatctg ttctggtata actccccgac ccctaatttt aatggtggcg tgaacgttat
     3721 gttctgggtg attttcatcc tgatttttgt cggactggcg ttgcaggatt ccggtgcccg
     3781 gatgagtcgc caggcgcgct tcttgcggga aggtgttgaa gatcaactca ttctggaaaa
     3841 agccaaaggg gaagaggggc taacgcgtga acaaattgag tcccgtattg ttgttccgca
     3901 ccatactatt ttcctgcagt ttttctcact atatatcctg ccggttattt gtattgctgc
     3961 aggttatgtg ttcttttctc tgcttgggtt tatttaatca aaaggccgca cggagcggcc
     4021 attatcctca acccatatca tgctgctaat aatctatcca atgactgctg agcaataacc
     4081 aaatgctgac cgccaaataa tcttgcacga tttaacagcg tgtagagttg gtaaaccggt
     4141 tgacgttcaa ggaaatcggc aggtagcggg gatactgact gatagccgtc atagatttgt
     4201 ggcggctgtt cagtatgcag cggtaacatc gccaggtcgc actctcggtc accccagtag
     4261 caggcagggt cgaaaatgta cgggccatcc ggacccagcg cacagttgcc ggaccataaa
     4321 tcgccgtgca acagagaagg ctgcggttga tgcgaggcca gacgctgctg aatatgctcg
     4381 acgatggcgt cgatattgcc gaaagcgatc cctttctctg ctgccagttc caactgccag
     4441 ccaatccgtt gttcagcaaa aaacgttgac cagcgacgtt gccaggtgtt gggctgtgga
     4501 gttgtggaga gcgagttatc aaaatcgagg ccaaattgtg gttggtcact ccattgatgt
     4561 aaacgcgcaa tttgctgacc aagaataaat gcactatgcg catccagcgg acgaggtggg
     4621 agataatcca tcaccagaaa actgtagtca cggtcagcgc ctactgccca aaccttaggc
     4681 acggtgacgg ttttactacg cgacagtaac tccagttggt cggcttcggc ggtaaaaccg
     4741 ggaagcagtt ccctttcatc acatttgacg aaaaagtcat gtcctgcata gcgcaaatgc
     4801 catgcggcat ggacttctcc gccaggcagt tcattacgca gttcgatttc gccttcacct
     4861 aactgctcgc tcaaaagacg actgattgcc tgccacatga tgtctctccc atgttgtctg
     4921 ccagatcata aggttagcgc aagaatgcgg tgtaaaaaca cgagctaacg cacaccacag
     4981 actgattatt gcccaaaaac catttatttt ctgttgctat tccattcctc ccaggtcgtg
     5041 atggtgattt caggatcttt gccggttgca ctttgggtaa gccccgaaac cagttctcta
     5101 atttcttctt cactttgggt actgataagt ccaaaagtgt tagtccccag ctcatggata
     5161 tttccctcat catccgtcat ggtgagcaaa aaaccgtcgc gagtcaggta attattcaac
     5221 tcgttaattt cagtcaatgt atcctcatgc aacatgacgg ttatgacata acggacaaga
     5281 tcgccactgg ccatagttca cccctttgtt atcacatgac ttttgagcat agtcggagaa
     5341 acgcgttgcc gacaggttgg tggtgattcc cccaatgctg ggggaatgtt tttgttagcg
     5401 ggaaaggtaa gcgtaaattt tttgcgtatc gtcatgggag cacagacgtg ttccctgatt
     5461 gagtgtggct gcactccccg cagctacgcc aaaacggacc atctcttcaa gagaggcatt
     5521 ttctgccagt ttcagtgtca tcgcgccgac catgctgtca ccagcgccaa cggtactctg
     5581 gcttttcacc ggtggtggca ccacctgaat gcagttttca ctatcaacac ccagcgctcc
     5641 ttgtggacca agggaaacga caacccgttt ggccttgccg ctattaacga tttcctgcgc
     5701 ggctttgcgg acatcgtctg gctgggtgag ttcgcgattc accagcgcac tgagttcttt
     5761 ttggttaggc ttaaccaact cgatgttacc aatcgccagt gctgcactta acgcttcgcc
     5821 agaactgtcg atgatgcagc ggatcccttg gttttgcgca gcggaaatca gttgggttaa
     5881 tttttccagc ttcacacctg gcggcaggct tccgcttatg accaggatgg ccccggattc
     5941 aatttccaga acttgctctt caagctggcg aaactcatct tcatttaatg ccgcgcctgg
     6001 cataacaaaa cgatactgct caccgcttgc ttccacatgt acgtgtaaat tctgccgggt
     6061 ccagtctttg gcttctacag tagcgacggg gacattttca tccgccaaca gagaaaccag
     6121 gtgttcgccg gtcgcgccac ccgccgggaa gatcgctgtg gcactgcctc caagatgggc
     6181 aatggcgcgg gcgacgttga tgccgccgcc cccgggttcg aacaccggtg cggtacagcg
     6241 cagttttcct tcgggataaa tttgcggggt aattgttgcg ctatcgagag agggcgcaag
     6301 tgtcaacgta tagatacgta ccatcatttc ctcctatagg ctgatttcag tctggcaccg
     6361 aatcagccga aagtgaagaa
//
LOCUS       DAPGWD010000209         6366 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPGWD010000209 DAPGWD010000000
VERSION     DAPGWD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.211"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(385..2055)
                     /gene="betA"
                     /locus_tag="RHX88_004095"
     CDS             complement(385..2055)
                     /gene="betA"
                     /locus_tag="RHX88_004095"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDV2232713.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIREQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(2069..3541)
                     /gene="betB"
                     /locus_tag="RHX88_004096"
     CDS             complement(2069..3541)
                     /gene="betB"
                     /locus_tag="RHX88_004096"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDV2232714.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRGTSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3555..4142)
                     /gene="betI"
                     /locus_tag="RHX88_004097"
     CDS             complement(3555..4142)
                     /gene="betI"
                     /locus_tag="RHX88_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDV2232715.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4271..6304
                     /gene="betT"
                     /locus_tag="RHX88_004098"
     CDS             4271..6304
                     /gene="betT"
                     /locus_tag="RHX88_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDV2232716.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1302 a         1757 c         1759 g         1548 t
ORIGIN      
        1 ttaaagaaga gtaaaaataa atcactgcca ataaattata cgaatcgtgt ttaaagctcc
       61 acgttgtttt ggtctgactt atttttgtcc tcgtttcaag aacccttttc gtatatgcta
      121 accgtgagcg ggagtgaaca agtcaccata gccaggaaca ctagcggcgt aattcttaag
      181 ctgagtggca tttcacctgc ggtttatcag tgcattcata gcaccttcat cagccagatt
      241 gaacgagtcc gcttagaatt tgctggcgag gttgtcacta tgaagcatga gagttacccc
      301 tatgttttat ataacgattc tgtccccata cctggaacgg taactctcaa tcagatcggt
      361 tcttgagtca gttcaaatca cgactcattt tttcgctctc accggcatcc catttgccac
      421 aaaatatccc gccgtgctcc tcggcagcgc ttcctgttca cgaatcatat ccgctatttt
      481 ctcgccaatc ataattgtcg tggcgttcaa attcccggtg ataatctgcg gcataatcga
      541 cgcatccacc acacgcaggc cttctaaccc gtgtacgcgg ccttcgccgt caaccacgga
      601 catctcgtcg taacccattt tgcaggtacc gcacggatgg aaggcggttt cggcgtggtt
      661 acgcacgaac tcatcgagct gttcatccgt ctgacattcc gtgccggggc tgatttcgcg
      721 gccacgatac tgatccagcg ccggttgatg catgatctcc cgggtgatgc gaattgcgtc
      781 gcggaactcc tgccagtcct gctcgtgtga catgtagtta aacagaattg ccggatgctg
      841 gtgcgggtcg cgggatttaa tccgcacatg cccacggctt ggcgagcgca ttgagccgac
      901 gtggcactgg aaaccgtgct ccttcaccgc attcgagccg ttatagttaa tcgctactgg
      961 caggaaatgg tactgaatat tcggccacgc aaattcctct cggctgcgaa taaatccgcc
     1021 tgcttcaaag tggttgctgg caccaacgcc agtgccgcca aacagccact ccgcaccgat
     1081 tttcggctgg ttccaccact gcagggcagg gtagagggaa accggttctt tgcactcata
     1141 ttgcagatac atctccagat gatcctgaag attttcgccg acgccgggta attcatgcac
     1201 cagcggaata tcaaactccg ccagcagttc agcgttgccg acgccggagc gttgcaggat
     1261 ctgcggtgag gcaatcgcgc ctgcacataa cagcacttct ttgttggccg ttgcgcgggt
     1321 tgggatggtg ctgtcgcctt ccagccattc cacgcccacc gcgcgtttgc cgtcaaaaat
     1381 gatgtgatcg gtcatagcgt gagtacgaat ggtcaggtta gggcgcgatt tggcctgatc
     1441 gagataaccg cgcgcggtac tggcgcgacg gccctgcggc gtgacggtgc gatccatcgg
     1501 gccaaaacct tcctgctgat agccgttgag atcgtccgtg cgcgggtagc ctgcctgcac
     1561 gcccgcttca atcatcgctt caaacagcgg attgacgccg ggtttggagg tggtgacgct
     1621 caccgggcca tcgccaccgt ggtagtcgtt ctcgcccaca tcgcgagtct cggccttgcg
     1681 gtagtagggc aggcagtcga gataactcca gttctccaga ccgggttctt gcgcccagtt
     1741 atcgagatcc agcgcattgc cacggatgta gcacatgccg ttgatcagcg acgatccacc
     1801 caggccttta ccgcgtccgc actccatgcg gcggttattc ataaacggtt caggctctgt
     1861 ttcataggcc cagttgtagc gtttgccctg tagtgggaac gccagcgctg cgggcatctg
     1921 ggtgcggaag tcaaagcgat agtccgggcc gcccgcttca agcagcagca cggaggtatt
     1981 cggatcttca gtcagacggg tagcgagaac gttgccggct gagccggcac caataatgat
     2041 gtagtcaaat tgcaaataaa cctcctggtt agaatatgga ctggaattta gccatctcaa
     2101 cctggatgga cttcacctgg gtgtaactct ggagcgtcat cacgccgttc tcgcgaccaa
     2161 tgccggagtg tttgtagccg ccaacgggca tctctgccgg ggattcgccc caggtgttga
     2221 tccagcaaat acccgcttcc agctgatgaa tgacgcgatg cgcgcggttc aggtccgccg
     2281 tcacgatgcc cgccgccagg ccgtagtcgg tatcattggc gcggcgaatc acttcgtcct
     2341 cagattcata agtgagaagc gacatcaccg gaccgaagat ctcttcgcgc acgatggtca
     2401 tttcgtcgcg gcaatcggtg aacacggtcg gtgcaaccca tgcgccgtta tcgaagccat
     2461 cgcctttcag tacatcgccg ccgcacagta cgcgcgcgcc ttcctctttg cctttggcga
     2521 tatagcgcag cacgttatcg cgatgcggga agctgaccag cgggccgaaa ttggtttgcg
     2581 gatcgaaaac gtcgcccgcg cgaatgcgct caacgcgcgc cagaattttc tgctcaaatg
     2641 cggctttgca tttcgccgga acgaagacgc gggtgccatt ggtacacacc tgaccggagc
     2701 tgaagaagtt tgccatcatg gcgatatcgg cggcgagatc gagatccgca tcatcgaaaa
     2761 cgatcagcgg tgatttaccg cccagttcca tggtcacttc tttcagggaa gaggccgccg
     2821 agttagccat cacttttttg ccgctggcga caccgccggt aaatgacact ttggcaatgc
     2881 ccggatgctc ggtcagatat tgcccggtct ccgcgcccac ccccggcaac acgttaaata
     2941 cgccgtccgg caggcccgct tcgctgtaaa tttcagccag ctttaacgcg gtaagcgggg
     3001 tgacttcgct cggtttgaaa atcattgcat tgcctgccgc cagcgcgggg gcggatttcc
     3061 atagggcaat ctggatcggg tagttccatg cgccaatccc cgccaccacg cccagcggtt
     3121 cgcggcgggt ataaacaaat gacgtcccac gcaacgggat ctggctgcct tccagcgacg
     3181 ggatcagccc ggcatagtac tccagcacgt ccgcaccggt aacgatatcg acggttgagg
     3241 tttccgaata tgcttttccg gtatcgaggg tttcaagttt tgcgagttcg tcattgcgtt
     3301 cacgcagaat atcaacggcc cgacgcagaa tacgcgagcg ctccatagcg gtcatcgccg
     3361 cccagatttt ttgcccctgt tgggcactct tcacggcgcg atcgacatcc tcacgcccgg
     3421 cggcctgcac ggtcgccagc acgttaccgt tggccgggtt aatggtctcg aaggtgcgac
     3481 cgctggtggc ggaggtataa ccaccatgta tataaagctg ctgttctgcc attcgggaca
     3541 tcacgtctcc tcggttaatc ggtgggtaaa tgctgagtaa taaagtgacg ggtcagcgag
     3601 tgtgcgagag gtttatccag cggtttgccg ctcagtgccg cgcgcagcca taatccgtca
     3661 atcagcgcgg caaggccgta gcccgcttcc tgtgcctgtt ggcgcggtaa ctcgcggcga
     3721 aattcgctca ccagattcga caataagcgg cggctactga cctgctgtaa acgatagagc
     3781 atcggctgat gcatactgct ggcccagaac gccagccagg ctttcatcgc cgcactgctc
     3841 acctgcgttt catcgaagtt tccgccaaca atcgcctgta atcgctgctc tgcactgccc
     3901 tgcggaagtg catgtaatcg attcaaaacc gcgtcacgca gctgactggt gatatcgcgc
     3961 atggttgctt ccagcagacc atttttgtcc ctgaaatagt ggctgatgat ccccgtagaa
     4021 acgcctgcac ggcgggcgat ctgcgcgatc gttgcatcgt gcatgcccac ttcatttatt
     4081 gcttccagtg tggcgtcgat cagttgtctg cgccggatcg actgcatccc caatttgggc
     4141 attttcgcca ctccattcat cagcgttgtt tatctattaa agcggttatt gattggacgt
     4201 tcaatataaa atgtgtctta attgttacga atttgatttt aaatggtaac aataacagtg
     4261 gggatactgg atgacagacc tttcacacag cagggaaaag gacaaaatca atccggtggt
     4321 gttttacacc tccgccggac tgattttgtt gttttccctg acaacgatcc tgtttcgcga
     4381 cttctcggcc ctgtggattg gccgcacgct ggactgggtt tctaaaacct tcggttggta
     4441 ctatctactg gcggcaacgc tctatattgt ctttgtggtc tgtatcgctt gttcgcgttt
     4501 tggttcggtg aagctcgggc cagaacaatc caaaccggaa ttcagcctgc tgagttgggc
     4561 ggcgatgctg tttgctgccg ggatcggtat cgacctgatg ttcttctccg tagccgaacc
     4621 ggtaacgcag tatatgcagc cgccggaagg cgcgggacag acgattgagg ccgcgcgtca
     4681 ggcgatggtc tggacgctgt ttcactacgg cttaaccggc tggtcgatgt atgcgctgat
     4741 gggcatggcg ctcggatact ttagctatcg ttataatttg ccgctcacca tccgctcggc
     4801 gctgtacccg atcttcggta aacggattaa cgggccgata gggcactcag tggatattgc
     4861 agcggtgatc ggcaccatct tcggtattgc cactacgctc ggtatcggtg tggtgcagct
     4921 taactatggc ttgagcgtac tgtttgatat tcccgattcg atggcggcaa aagcggcact
     4981 gatcgccttg tcggtgataa tcgccacgat ctctgtcacc tccggtgtcg ataaggggat
     5041 tcgtgtgtta tcggaactta acgttgcgct ggcgctggga ttgatcctgt tcgtattgtt
     5101 tatgggcgac acctcgttcc tgcttaatgc gctggtgctg aatgttggcg actatgtgaa
     5161 tcgctttatg ggcatgacgc tcaacagttt tgccttcgac cgtccagttg agtggatgaa
     5221 taactggacg ctcttcttct gggcatggtg ggtggcatgg tcgccgtttg tcggcttgtt
     5281 cctggcgcgt atctcgcgtg ggcgtaccat tcgccagttc gtgctgggca cgttgattat
     5341 tccgtttacc ttcacgctgt tatggctctc ggtattcggc aatagcgcgc tgtatgaaat
     5401 catccacggc ggcgcggcat ttgccgagga agcgatggtc catccggagc gcggcttcta
     5461 cagcctgctg gcgcagtatc cggcgtttac ctttagcgcc tccgtcgcca ccattactgg
     5521 cctgctgttt tatgtgacct cggcggactc cggggcgctg gtactgggga atttcacctc
     5581 gcagcttaaa gatatcaaca gcgacgcccc cggctggctg cgcgtcttct ggtcggtggc
     5641 gattggcctg ctgacgctcg gcatgctgat gaccaacggg atatccgcgc tgcaaaacac
     5701 cacggtaatc atggggctgc cgttcagctt tgtgattttc ttcgtgatgg cggggttgta
     5761 taaatctctg aaggtagaag attaccgccg tgaaagtgcc aaccgcgata ccgcgccgcg
     5821 accgctgggg cttcaggatc gcctgagctg gaaaaaacgt ctctcgcgcc tgatgaatta
     5881 tccgggcacg cgttacacta aacagatgat ggagacggtc tgttacccgg caatggaaga
     5941 agtggcgcag gagctgcggt tgcgcggcgc gtacgtggag ctaaaaagcc tgccgccgga
     6001 agagggacaa cagttggggc atctggattt gttggtgcat atgggcgaag aacaaaactt
     6061 tgtctatcag atttggccgc agcaatattc ggtgccgggc tttacctacc gcgcacgtag
     6121 cggtaaatcg acctactacc ggctggaaac cttcctgtta gaaggcagcc agggcaacga
     6181 cctgatggac tacagcaaag agcaggtgat caccgatatt cttgaccagt acgagcggca
     6241 ccttaacttt attcatctcc atcgtgaagc gccgggccat agcgtgatgt tcccggacgc
     6301 gtgattgttt ttgcataaaa ccgcgccatg tctgcatatg gcgcgggaat cattgactcg
     6361 ctgtaa
//
LOCUS       DAPGWD010000210         6282 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPGWD010000210 DAPGWD010000000
VERSION     DAPGWD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.212"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(29..181)
                     /locus_tag="RHX88_004099"
     CDS             complement(29..181)
                     /locus_tag="RHX88_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV2232717.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(446..1180)
                     /gene="cysH"
                     /locus_tag="RHX88_004100"
     CDS             complement(446..1180)
                     /gene="cysH"
                     /locus_tag="RHX88_004100"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV2232718.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1254..2966)
                     /gene="cysI"
                     /locus_tag="RHX88_004101"
     CDS             complement(1254..2966)
                     /gene="cysI"
                     /locus_tag="RHX88_004101"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDV2232719.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2966..4765)
                     /gene="cysJ"
                     /locus_tag="RHX88_004102"
     CDS             complement(2966..4765)
                     /gene="cysJ"
                     /locus_tag="RHX88_004102"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDV2232720.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLKNTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHSSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5084..5446
                     /gene="queD"
                     /locus_tag="RHX88_004103"
     CDS             5084..5446
                     /gene="queD"
                     /locus_tag="RHX88_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDV2232721.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5486..6040
                     /locus_tag="RHX88_004104"
     CDS             5486..6040
                     /locus_tag="RHX88_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDV2232722.1"
                     /translation="MLGCCVLDIWGMTWKTTATLLLLVPVLQAPLARYAWIYRSILES
                     VALMLKNNYDNMRNEMNHFAKHVIITGGGSNSDLFMQIFADMFNLPARRNAINGCASL
                     GAAINKAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRYDAMNKGIFKDLTKHNDVI
                     LKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         1448 a         1668 c         1601 g         1565 t
ORIGIN      
        1 tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct
       61 taaactccat accacgttct ctgatactca actcacacaa cgagtcacct accatcagcg
      121 tgaatcccag tactgtacaa cacagtacga tgattgccac aagggcatat tttgtcagca
      181 tgtcttgcct ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgtaagc
      241 ctcaggttga tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta
      301 acgccagaca gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt
      361 tcacgtttaa aatcaggaat actgctcgca aaataaaaac gcccccatca tttatgacag
      421 aggcgtttaa tttatccggc aatatttacc cttcgtgcaa tccacattcc ctttttaagc
      481 caaagaaacg tgtttcttct tccgacatgc caggttccca tttacgggtt gtatgggtat
      541 caccgaccga taaatatcct tcatcccata atgggtgata tttcaggcca tgtttttgca
      601 gatactgata tatcgttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac
      661 gctgaattgc cagcaccggt aaattggcgc gactgccgga ttgttcacgg cgcaggccag
      721 caaaccaggt ttgcgcgtta agttctttca gagcccggtt catcggttcg actttgttga
      781 tgtcattgta cttttcaatg ccttcaacgc cctgttccca cagtttgccg tagcgtgctt
      841 cctgccaggc ggcgctttcg gtagcgcggt acactttcag gttgagcttg agtttgtccg
      901 ttaactcgtc aataaagcgg taggtttccg ggaacagata gccggtgtcg gtgaggatca
      961 ccggaatatc cgggtgaatc tgattcacca gatgcaggct caccgccgcc tgaatgccaa
     1021 agctggaaga aagtacatat tcaccgggca gattatccag cgcccaggca acgcggcctt
     1081 cagcgtccag tttttccagt tcggcgttag tttctgccag cgccagaatg cgatctacct
     1141 tcggcagttc gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct
     1201 tgcctgatgc gacgctcacg cgtcttatca ggcctacaag accgggctga tggttaatcc
     1261 cacaaatcgc gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg
     1321 ccgaagcctt cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac
     1381 gccaggattt ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg
     1441 cggttgccgc caagatgcag gttgtagcga cccggcgctt tacccaccag gcccacttcc
     1501 gccagcatcg cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca
     1561 tcgctgacac catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa
     1621 cgctctgctt ccgccatcgc cagcgggcaa gtcgggaatg acacgcaggc catcgagttt
     1681 tcacgctgcg gcgtgacggc attcattaaa ccgctctctt tggcgatctt ctcgatcttc
     1741 gctttctcgc tttccggtac accggcgatg atcagattct ggttcgccgt aatgcggaaa
     1801 tcgcctttgt ggatcttcgc gatctccagc aggccggttt tcagcggacg ccccggataa
     1861 tcaaggatgc gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta
     1921 acccagccaa tacgatcgcc tcgtccggtg aactcatatg gacggatcgg ttcaaatttg
     1981 atccccgcgc gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg
     2041 tatttggttt tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg
     2101 acggcttcgg ccaccgccag cgtatgctcc agcggcaaat agccaaactc gctcgccgtg
     2161 cgggcgtagg ttttcttgtt gccgtgttcg atagaaagcc caccgcccac cagcaggttg
     2221 aagcctacca gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt ggcatgcaga
     2281 tcgatatcgt tctgcggcgg gatcactacc gtggttttga acttacgcgg caggtaggtc
     2341 tgaccaagga tcggttcttc atcagtagtg gcgacttttt cctggtcgag ccagatctcc
     2401 gcatacgcgc gggtacgagg caacagatgc tcagaaatct tcttcgccca ctcgtacgct
     2461 tccgcgtgca gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg
     2521 ttagctgtcg ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg
     2581 ttctttttca gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg aatgctgcca
     2641 tagatggtgt tttcaccggc aaatttgtcg atcgcctgcc actgtttagt ggtaatcacc
     2701 ccacccggca gacgacagcg aagcagcatc gcgtggcgcg gctccagctt ctgttcagca
     2761 cgttcggcgc ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg
     2821 aagttatcgc ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg
     2881 cgcaggtagt tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact
     2941 aaaggccctg gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc
     3001 gcagctcact taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca
     3061 cttccagaag tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg cagacataaa
     3121 tgtgggcacc atcattgatc cagcgccaca gctccgcgcc ctgttcgcgc agtttgtctt
     3181 gtacgtaaac tttttctttt tggtcgcgcg accaggcaag atcgatacgt gtcagcacgc
     3241 catctttgac gtagcgctgc cactccacct ggtacaggaa gtcttccgta aagtgcggat
     3301 taccaaagaa cagccagttt ttacctggcg cttcgtcggc ggcgcgttgc tgcataaagg
     3361 cgcggaacgg cgcaataccg gtgcctgggc caatcataat caccggggtt tccgggttag
     3421 cgggcaggcg aaagttatcg ttatgttcga taaatacgcg gacttcgccc tcttcttcca
     3481 cgcgatccgc gaggaagctg gaggcaccac cggcacgggc tcggccttcc acgtcgtaac
     3541 gcaccacacc aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag gaggcgatgg
     3601 aatacaggcg cggggtcagc gggcgcagca gattaatcag cgcttcggca tccagttgcg
     3661 ccggagagaa acgcaccatg tcgacaatcg gcgtcgtcgc ggcgtaatgc tgtaacttcg
     3721 ctttatcgcc caccagcggc agcagtgttt cgctgcgggt aagcgtggcg taattctcaa
     3781 caatgttggc ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca
     3841 acgttttgcc ctcgacggtg acaggttcat cgcctttcag ccacagcagt tcgacaagtt
     3901 ctttcaccag tgccggatcg ttctgatacc agacgcccag cgcgtcaccc ggctggtaac
     3961 gcaggcccga gtcacctaag tcaatttcga tatggcgaac gtctttttca gagttacgtc
     4021 cggtaatttt ctggttaacc gaaaggctcg ccaccagcgg agcgtctttg ctgtacgggc
     4081 tggagtggat ttcatttacc gcgccagtag cgacggattg cgaaggtgcc gcgacaggcg
     4141 cacgcgattt aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt
     4201 caacatcggc atcgacacgg tcgagcaggc gttcaccgcc cagttccgcc agcttgctgt
     4261 caaaatcttt cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg
     4321 caaatgcggt gtttttcagc ttcggcgctt ttttggagaa caggaactta tgcagcgcga
     4381 cggcttcttc cggcggttcc ccttctcctt gcgttgacgt caccacgata agcagttttt
     4441 cgctggcgat ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg
     4501 ctgctaataa atcatcgcgt aatgcttcag caacccggcg cgcattgccg gtttgcgagg
     4561 cggagataat agttataccc ggcatttctg cggctggcgc tggcgtcgct gcaagcgcag
     4621 caggctgctg attgagtacg ccccagaaat agccagaaac ccaggcaagc tgggtgggag
     4681 ttaaatcggt cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac ggaagcaacg
     4741 cggaaggtgg gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg
     4801 cgccctgtca gcgcccataa aacagaagag aaggtaaggt taacggggtg aacggtgtgg
     4861 attaaagacg ggatagcgat aactaataac caaatcgact aacctgtttt agcaatagtc
     4921 tttaacaaca aaatagatta accaacctaa tgaaaaacaa atgaatttag ccaatcatta
     4981 agataaatca gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa aacagccttt
     5041 ccggtactct acggcggttt tatcgtcctg tagagaaatt atgatgtcca ccacgttatt
     5101 taaagatttc accttcgaag ccgctcaccg cttaccacac gtcccggaag ggcataaatg
     5161 tggtcgcctg cacgggcatt cctttatggt gcgtctggaa attaccgggg aagtcgatcc
     5221 gcatacgggc tggattatcg atttcgctga actaaaagcg gcgtttaagc caacttatga
     5281 gcgcctcgat caccattatc tcaatgatat tccaggtctg gaaaacccaa ccagcgaagt
     5341 tttagcaaaa tggatttggg atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat
     5401 ggtaaaagaa acctgcaccg ctggttgtat ctatcgcggc gaatgataag agtgtgtcgg
     5461 cggtcaattt cccttaagta acgctatgtt agggtgttgt gttctggata tctggggcat
     5521 gacatggaaa acgactgcga cattattatt attggtgccg gtattgcagg caccgcttgc
     5581 gcgttacgca tggatatatc gttcgatact ggaaagcgtg gctctgatgc tgaagaacaa
     5641 ttacgacaat atgcgtaatg aaatgaatca ctttgcaaag catgtgatca ttactggcgg
     5701 cggttcgaac agcgatctgt ttatgcagat ttttgccgac atgttcaacc ttccggcacg
     5761 acgtaacgcc attaacggtt gtgcaagtct gggggcagcg attaataagg cggtaggtct
     5821 gggactatac ccggattacg caacggctgt cgacaaaatg gttcgcgtga aagatatctt
     5881 tataccgatt gagagcaatg ccaaacgcta cgacgcgatg aataaaggca ttttcaaaga
     5941 cctaaccaaa cataatgatg taatactgaa aaaatcgtat gaagtgatgc atggggaatt
     6001 ggggaatgtg gattcgatcc agagctggtc gaatgcgtaa gtgaggaagg ccgggcggga
     6061 aactgcccgg cctgaacata cctgaatggt tatccccgct gacgcgggga acataagttc
     6121 gacaacttaa aaaatgataa caagtccggt ttatccccgc tgatgcaggg aacataataa
     6181 aaactttaac tagttcggtt tatccctgtt tttacaagga atattaacta cactcgatgt
     6241 ctttagaata aaagacaata cgaatcgcac tttatcaatt tt
//
LOCUS       DAPGWD010000211         6273 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPGWD010000211 DAPGWD010000000
VERSION     DAPGWD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.213"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            77..214
                     /locus_tag="RHX88_004105"
     CDS             77..214
                     /locus_tag="RHX88_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HDV2232723.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAV
                     Q"
     gene            complement(1241..1432)
                     /locus_tag="RHX88_004106"
     CDS             complement(1241..1432)
                     /locus_tag="RHX88_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HDV2232724.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1496..1819
                     /locus_tag="RHX88_004107"
     CDS             1496..1819
                     /locus_tag="RHX88_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDV2232725.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            1761..2519
                     /locus_tag="RHX88_004108"
                     /pseudo
     CDS             1761..2519
                     /locus_tag="RHX88_004108"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2497..3015
                     /locus_tag="RHX88_004109"
     CDS             2497..3015
                     /locus_tag="RHX88_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="HDV2232726.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSN
                     ETKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     PFQGKEWVHLKE"
     gene            3022..3255
                     /locus_tag="RHX88_004110"
     CDS             3022..3255
                     /locus_tag="RHX88_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HDV2232727.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(3573..3992)
                     /locus_tag="RHX88_004111"
     CDS             complement(3573..3992)
                     /locus_tag="RHX88_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HDV2232728.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
     gene            complement(4073..4510)
                     /locus_tag="RHX88_004112"
     CDS             complement(4073..4510)
                     /locus_tag="RHX88_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HDV2232729.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGR
                     REFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDK
                     QAQDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
     gene            4770..>6273
                     /gene="cea"
                     /locus_tag="RHX88_004113"
     CDS             4770..>6273
                     /gene="cea"
                     /locus_tag="RHX88_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="HDV2232730.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSK
                     SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEAL
                     RHNASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEI
                     EREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSR
                     LSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRR
                     RVGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLK
                     KAQNNLLNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAA
                     FEKYKDVLNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSD
                     ILKIKDTGDWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVT"
BASE COUNT         1628 a         1375 c         1740 g         1530 t
ORIGIN      
        1 atatttttta tgtagtatct taagctcata aattaaacct cgccatatat tcttttcatt
       61 ttataaggat cgagttatga ggaaaagatt ttttgtggga atattcgcga taaacctcct
      121 tgttggatgt caggctaact atatacgtga tgttcaggga gggaccatcg caccatcctc
      181 ctcttctaaa ctgacgggga tcgcggttca gtagaaaaga ttaaaggatc ttcttgagat
      241 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accaacggtg
      301 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      361 gcgcagatac caaatactgt ccttctagtg tagccgtagt cgggccacta cttcaagaac
      421 tctgtagcac cgtttgtgcc atcatcgctc tgctaatccg gttaccagtg gctgctgcca
      481 gtggcgttaa ggcgtgcctt accgggttgg actcaagacg atagttaccg gataaggcgc
      541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      601 ccgaactgag ataccaacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      781 gtctattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc tgctacgtgg
      841 ccttcttcct gttcctggtc ttttgctcac atgttctttc cggccttatc ccctgattct
      901 gtggataact gtgttaccgt ttttgtgtga gtcagtaccg ctcgccgcag tcgaacgacc
      961 gagcgtagcg agtcagtgag cgaggaagcg gaaaagcgcc tggacgtgca ttttctcctt
     1021 acgcatctgt gcggcatttc acacccggca tggcgtactt ttcatacaat ccgcactgat
     1081 gccgcatggt taagccagta tacactccgc tatcgctacg tgactgggtc agggctgcgc
     1141 cccgacaccc gctaaaacct gctgacgcgc cctgacgggc ttgtcagctc ccggcatccg
     1201 ctcacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     1261 ccccgaaacg tgcgaggcag ctgcggtaaa gctcatcggc gtggtcgtga agcgattcac
     1321 aaatatcggc ctgttcatct gcgtccagtt cgttgagctt ctccagcagc gttaatgtct
     1381 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttagtc actgatgcct
     1441 ccgtgtaagg gggatttctg ttcatggggt aatgataccg atgaaacgcg agaggatgct
     1501 cacaatacgg gttactgatg atgaacatgc ccggttactt gaacgctgtg agggtaaaca
     1561 actggcggta tggatgcggc gggtctgcct gggggagccg gttgcccgtt ccggaaaact
     1621 gccgacactg gcaccgccgt tactgcgtca gctggccgcc atcggaaata acctgaatca
     1681 gacagcccgt aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc aggtggtggc
     1741 cgcactgatg gccatcgggg atgagctgcg ccggctgcgt ctggctgtca gggaacaggg
     1801 ggcgcgggat gatagttaaa tttcatgcca ggggaaaagg tggtggcagt ggtccggttg
     1861 attacctgct ggggagggag cgtaaccgcg aaggcgcaac ggtgcttcag ggtaatccgg
     1921 aagaagtccg ggaactcatc gatgccacgc catttgcgaa gaaatacacg tccggtgttc
     1981 tgtcgttcgc ggagaaggag ctgccgccgg gaggacgtga aaaagtgatg gcgagctttg
     2041 agcgtgtact gatgcccggt ctcgaaaaaa atcagtacag catcctgtgg gtggagcacc
     2101 aggacaaggg acggcttgag ctgaattttg tcattccgaa catggagcta cagaccggaa
     2161 aacgcctcca gccgtactac gaccgcgcag acaggcctag aattgatgcc tggcagacgc
     2221 tggtaaatca ccattacggg ctgcatgacc cgaacgcccc ggagaaccgc aggacgctga
     2281 cactccctga taacctgcct gaaacgaaac aggcgcttgc tgagggcgtc acgcgaggta
     2341 tagatgcact ttaccatgcc ggagagataa aaggccgtca ggatgtgatt caggcgctca
     2401 ctgaggcggg gctggaagtg gtcagggtga cgcgaagcag tatcagcatt gcagatccga
     2461 acggcgggaa gaatatcagg ctgaaaggag cattttatga gcaatctttt gcagacgggc
     2521 gcggagttcg agaaaaagct gaaagagaga gccgaatcta cagagaaaat gctgaacaac
     2581 gagttcagga ggctcggcga atctgtaagc gaggctgtga catcaaacga gacgaaaatc
     2641 agagacgcta tagccctgtt cacagcctcg acagaggaat cgctggaaaa acaccgggaa
     2701 ggggtgaaag aggcgatgat gcagcacagg agggacgtgt taaagctggc agggaatacg
     2761 ggcatgatgt tactggggat agtctttctc ctgtttaccg cgagtggcgg gacgctctgg
     2821 tatcttggag ggaggataca ggcgaacctg gaagaaatca ggaagcagga agagacattg
     2881 cagaaactga acgcgaagac atggggcgtg gagtttgtgc aggacgggaa caggaaattc
     2941 cttgtccttc cgtacgggaa atcagcggag gtgattccct ttcaggggaa agagtgggta
     3001 catctgaagg agtgacacag agtgacagag ctggaaacac atttgctgaa cgccttagag
     3061 cagctgcaac aggactatat gcagcggctg agcgaatggg agagcgcctt cgtggaattg
     3121 cagaagatgt tttcgcttac gcaacgggac aacgcgatgc tgaacgagcg ggtcatgcag
     3181 ttgagtcagc aggtgcagca cttgagcgag cagacagaac gcttgagcca gttatacagc
     3241 gagaactgga gataagagag gaacggctga tacaggagcg cgaacatgtg ttatccctgg
     3301 aacgggagcg tcagccggaa atacaggaac gcacgctgga tggcccttcg ctgggatggt
     3361 gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc
     3421 tggttgcata ggtattcata cggttaaaat ttatcaggcg cgatcgcggc agtttttcgg
     3481 gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg
     3541 tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta gccatcgaga
     3601 taaaccgcag aaatcgtgtc agccagcagc ctggattttt tcggggtaag gtctttgctg
     3661 cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg agtttaaaaa ttcctggctg
     3721 tgaaactgct gaatgcgttg ttgcacatca ctccgtgatt ttcctgtcgc tttatcttta
     3781 gctcccatat cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt gtcggctttt
     3841 tgggtggaaa agttgggatc aaaaccgtca tcagaagcat atacgtaatg ccgggtatgt
     3901 tgttccacgc tgccctttgg ggtacaggct gtgagagcta ctgttgctgt tatcatggcg
     3961 ataagacaaa gtttgtttcc ggtacgtctc atgagtgtct ccctacctta aagtatttat
     4021 tctcggtgag ttattatccg aagactattt tatcatcagt ctctgacagc gttcagcgac
     4081 cgaacaacct ggaccagaat ccccgtttct tttccagttc cagtgcctgc ctgagctggg
     4141 ctatctcatt ttgtagctgt tcccgttctg cttcctggcg tctgcgatcc atatcctgtg
     4201 cctgtttatc ctcaagcatc agcgtcaggc attgtttcag ctcattcagt tcccggagaa
     4261 tgcgttctgt ctgctgatca tgtggatttt ctgcatgtcc ctcactgtgc ctttctggtg
     4321 tctcattctg ctttaattcg ccgtatgccc ggatcagttc actggtttca aactcccgtc
     4381 gtccgtccct gccggtacgg taactgacaa ggcctgcgcg catatcacgg taaaactgtg
     4441 aacgcgatct gcctgtcatt tttagtgcgt cccggagtgt gtgccatgcc ataaagtgac
     4501 agtgtcccat agatgtctca tctcatagtt tcagtaaaac ataatgaggt ctgagaacgg
     4561 taatgtttgt gctggttttt gtggcatcgg gcgagaatag cgcgtggtgt gaaagactgt
     4621 ttttttgatc gttttcacaa aaatggaagt ccacagtctt gacagggaaa atgcagcggc
     4681 gtagctttta tgctgtatat aaaaccagtg gttatatgta cagtatttat ttttaactta
     4741 ttgttttaaa agtcaaagag gattttataa tggaaaccgc ggtagcgtac tataaagatg
     4801 gtgttcctta tgatgataag ggacaggtaa ttattactct tttgaatggt actcctgacg
     4861 ggagtggctc tggcggcgga ggtggaaaag gaggcagtaa aagtgaaagt tctgcagcta
     4921 ttcatgcaac tgctaaatgg tctactgctc aattaaagaa aacacaggca gagcaggctg
     4981 cccgggcaaa agctgcagcg gaagcacagg cgaaagcaaa ggcaaacagg gatgcgctga
     5041 ctcagcgcct gaaggatatc gtgaatgagg ctcttcgtca caatgcctca cgtacgcctt
     5101 cagcaacaga gcttgctcat gctaataatg cagctatgca ggcggaagca gagcgtttgc
     5161 gccttgcgaa agcagaagaa aaagcccgta aagaagcgga agcagcagaa aaggcttttc
     5221 aggaagcaga acaacgacgt aaagagattg aacgggagaa ggctgaaaca gaacgccagt
     5281 tgaaactggc tgaagctgaa gagaaacgac tggctgcatt gagtgaagaa gctaaagctg
     5341 ttgagatcgc ccaaaaaaaa ctttctgctg cacaatctga agtggtgaaa atggatggag
     5401 agattaagac tctcaattct cgtttaagct ccagtatcca tgcccgtgat gcagaaatga
     5461 aaacgctcgc tggaaaacga aatgaactgg ctcaggcatc cgctaaatat aaagaactgg
     5521 atgagctggt caaaaaacta tcaccaagag ccaatgatcc gcttcagaac cgtccttttt
     5581 ttgaagcaac cagacgacgg gttggggccg gtaagattag agaagaaaaa caaaaacagg
     5641 taacagcatc agaaacacgt attaaccgga taaatgctga tataactcag atccagaagg
     5701 ctatttctca ggtcagtaat aatcgtaatg ccggtatcgc tcgtgttcat gaagctgaag
     5761 aaaatttgaa aaaagcacag aataatctcc ttaattcaca gattaaggat gctgttgatg
     5821 caacagttag cttttatcaa acgctgactg aaaaatatgg tgaaaaatat tcgaaaatgg
     5881 cacaggaact tgctgataag tctaaaggta agaaaatcgg caatgtgaat gaagctctcg
     5941 ctgcttttga aaaatacaag gatgttttaa ataagaaatt cagcaaagcc gatcgtgatg
     6001 ctatttttaa tgcgttggca tcggtgaagt atgatgactg ggctaaacat ttagatcagt
     6061 ttgccaagta cttgaagatt acggggcatg tttcttttgg atatgatgtg gtatctgata
     6121 tcctaaaaat taaggataca ggtgactgga agccactatt tcttacatta gagaagaaag
     6181 ctgcagatgc aggggtgagt tatgttgttg ctttactttt tagcttgctt gctggaacta
     6241 cattaggtat ttggggtatt gctattgtta cag
//
LOCUS       DAPGWD010000212         6135 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPGWD010000212 DAPGWD010000000
VERSION     DAPGWD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.214"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            76..852
                     /gene="exuR"
                     /locus_tag="RHX88_004114"
     CDS             76..852
                     /gene="exuR"
                     /locus_tag="RHX88_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDV2232731.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1197..1859
                     /locus_tag="RHX88_004115"
     CDS             1197..1859
                     /locus_tag="RHX88_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2232732.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1863..2246
                     /gene="mzrA"
                     /locus_tag="RHX88_004116"
     CDS             1863..2246
                     /gene="mzrA"
                     /locus_tag="RHX88_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDV2232733.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2393..2761
                     /locus_tag="RHX88_004117"
     CDS             2393..2761
                     /locus_tag="RHX88_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDV2232734.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2799..3104
                     /locus_tag="RHX88_004118"
     CDS             2799..3104
                     /locus_tag="RHX88_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV2232735.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3107..3511
                     /locus_tag="RHX88_004119"
     CDS             3107..3511
                     /locus_tag="RHX88_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDV2232736.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3501..3800
                     /locus_tag="RHX88_004120"
     CDS             3501..3800
                     /locus_tag="RHX88_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDV2232737.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3986..4378
                     /locus_tag="RHX88_004121"
     CDS             3986..4378
                     /locus_tag="RHX88_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV2232738.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4448..5434
                     /gene="yqjG"
                     /locus_tag="RHX88_004122"
     CDS             4448..5434
                     /gene="yqjG"
                     /locus_tag="RHX88_004122"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDV2232739.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5727..6092
                     /locus_tag="RHX88_004123"
     CDS             5727..6092
                     /locus_tag="RHX88_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV2232740.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1533 a         1517 c         1547 g         1538 t
ORIGIN      
        1 tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt ccgcattcca
       61 ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca acttgccgct
      121 gacctgaaag agcgcatcga acagggcgtc tatctggtag gtgataaact gcctgcagaa
      181 cgctttattg ccgatgaaaa gaacgtcagc cgtacggtag ttcgtgaagc catcatcatg
      241 ctggaagttg aaggctatgt cgaagtgcgt aaaggttcag gtattcatgt ggtttccaac
      301 cagccgcgcc atcagcaggc agctgacaat aatatggaat tcgccaatta cggtccgttt
      361 gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgaattcgc ggcaactcag
      421 gtaacgaaac aggacatcat gaaactgatg gccattcagg aacaggcgcg cggcgaacaa
      481 tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct ggcgacgcag
      541 aactccgccc tggcggccat cgttgaaaaa atgtggacac aacgtagcca taacccgtac
      601 tggaaaaaac tgcacgaaca cattgattcc cgtaccgtcg ataactggtg tgatgaccac
      661 gatcaaatcc tcaaggcgct gattcgcaaa gatcctcatg ctgctaagct ggcaatgtgg
      721 cagcacctgg aaaacaccaa gatcatgtta ttcaacgaaa ccagcgacga cttcgagttc
      781 aatgccgacc gctatctatt cgccgaaaac ccggtggtgc atctcgatac ggccaccagt
      841 ggcagtaaat gaaatacctg tccgacaggc gctgcgacgc gcctgtttgt ctcgtcggta
      901 agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc ctgcattcag
      961 caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc tttgttacaa
     1021 ttagattcaa tttgaattta tgtttttgaa tgctttctta tctcacgatt taacagggaa
     1081 tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc aataacattg
     1141 cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg
     1201 aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc ctggccaatc
     1261 catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt gaaaacggct
     1321 tgcttccagc ggccttttta ccgggcgaca gtttactggt attggtcggc gtgttgattg
     1381 cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg
     1441 gctgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc gtacaaaact
     1501 ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt cataaacacg
     1561 gtttatcggc gctgttaatt ggtcgcttta ttgcgtttgt cagaacactg ctgccgacga
     1621 ttgccgggtt atcagggctg aataacgcgc gctttcagtt tttcaactgg atgagcggtc
     1681 tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg ccggtatttt
     1741 taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt
     1801 ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag
     1861 ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact gttttacttc
     1921 tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct acgctggcga
     1981 ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg catcaccttg
     2041 acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg ttaattacat
     2101 ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca ttgccccatg
     2161 gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg acccggttac
     2221 gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca ctcactttgg
     2281 tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc gcttcgttgt
     2341 ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa gaatgaaata
     2401 ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg ccactaccct
     2461 gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa aacaccaaaa
     2521 ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca actgttcaga
     2581 tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg aggtggcgga
     2641 acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgataaga ttgccaaacg
     2701 cgaacggaaa ctggcagaag cgcaggaaga gctgaaaaag ctggaagcgc gcgactacta
     2761 actcacaata gtcactactt actcacctgg agaaaactat gtcgaaagaa cacactacgg
     2821 aacatctgcg tgctgagttg aaatcccttt ccgatacgct ggaagaggtg cttagctcat
     2881 ctggcgagaa gtcgaaagaa gagttgagta agattcgtag caaagcggag caggcactga
     2941 aacagagccg ttatcgcctg ggtgaaaccg gtgatgccat cgccaaacaa acccgtgtcg
     3001 cggcggcgcg tgctgatgag tatgtgcgtg aaaatccgtg gaccggcgtg ggaattggcg
     3061 ctgcaatcgg tgtagtgctc ggcgttctgc tgtcgcgtcg ttaattatgg cggacactca
     3121 tcacgcgcaa gggcccggta aaagcgttct gggcatcggg cagcgaattg tttccatcat
     3181 ggttgaaatg gtggaaacac gtctgcggct ggcggtggtg gagctggaag aggaaaaagc
     3241 taatctcttt caacttttac tgatgctggg cctgacgatg cttttcgctg catttggtct
     3301 tatgagcctg atggtgctaa ttatttgggc ggttgatccg caatatcgcc tgaatgcgat
     3361 gattgccacc accgtggtgt tgctgctact ggcactgatt ggcggtatct ggacgttacg
     3421 taaatcgcgt aaatccacct tgctgcgcca tacacgccat gagctggcaa acgatcggca
     3481 actgctcgag gaggagtccc gtgagcagta aagtcgaacg tgaacgacgt aaggcgcaac
     3541 tgcttagcca gatccagcaa caacggctgg atctttccgc cagtcgtcgt gaatggctgg
     3601 aggcaacagg cgcttacgat cgtcgctgga atatgctgct aagtctgcgc tcctgggcgc
     3661 tggttggcag tagcgtgatg gcgatctgga cgattcgcca tcctaatatg ctggtccgct
     3721 gggccagacg cggttttggc gtatggagcg cctggcgtct ggttaaaacg accctcaagc
     3781 agcaacagct tcgcggttaa cttccctctg gccggagcaa ttccggcctt atccctcaaa
     3841 ttttttgaag atttttgaca gttttccttg ctaacaatca tcattcacca cgtttatgat
     3901 tctctccatc gacagcaacg acgctaatac cgcgcccttg cacaaaaaaa caatcagcag
     3961 cctgagtggc gcagtggaga gtatgatgaa aaaattagaa gatgttggtg tactggtagc
     4021 gcgcatttta atgccgattc tgtttattac cgcaggctgg ggaaaaatca ccgcttactc
     4081 tggtacccaa caatatatgg aagcaatggg tgtcccgggt tttatgctgc cgctggtgat
     4141 tctgcttgag ttcggtggtg gtctggcaat cctgttcggt ttcctgactc gcaccacagc
     4201 cctgtttact gcgggcttta cgctgctgac ggcattttta tttcacagca actttgctga
     4261 aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt tctggcggat tcctgctgct
     4321 ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg ctgaataaaa agtggtaagc
     4381 acgcgctata cttaacgata aaaaacgagg aggaagctcc tcgtttttgc tattggagga
     4441 gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac acctggtacg ataccaaatc
     4501 taccggcggt aaatttcaac gttcagcttc cgcatttcgt aactggctca ctgccgatgg
     4561 cgcacctggc cccactggca caggcggttt tatcgcagag aaagatcgtt atcatctcta
     4621 tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc atgcgcaaac tcaaaggact
     4681 ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg ctggaaaacg gctggacctt
     4741 tgatgacagt tttccgggag caaccggaga tacgctctat caacatgaat ttctgtatca
     4801 gctttatctc cacgccgatc cacactacag cggacgagtt actgttcccg tgctgtggga
     4861 caaaaagaac cacaccatcg tcagcaacga atcagcagaa atcatccgca tgttcaatac
     4921 cgcgtttgat gcgctgggcg caaaagcggg tgattactac ccaccagccc tgcaaacgaa
     4981 aattgacgaa cttaacggct ggatttatga caccgttaac aacggcgtgt ataaagctgg
     5041 ttttgccacc agccagcaag cctacgacga ggcggtggcg aaagtgtttg aatcgctggc
     5101 gcgactggag cagattttag gccagcatcg ttacctgacc ggcaaccagc taaccgaagc
     5161 cgatattcgc ctgtggacca cgctggtccg ttttgatcca gtgtatgtga cccacttcaa
     5221 gtgtgataag caccgcatca gcgattacct taatctgtat ggcttcctgc gcgatatcta
     5281 ccagatgccg ggaattgccg aaacagtcaa tttcgatcac atccgtaatc attacttccg
     5341 cagccataag accatcaacc ctacggggat tatttcaatt ggtccgtggc aggatctcga
     5401 tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag gcaccttacg aggtgcctct
     5461 gataattagc taagtgcatg ttgttcatgt cggatgcggc gtgaacgcct tatcaaacct
     5521 acaaaacatt tatattcaat atgttgcaat gaccgcgcag gccagataag cgtagcgcat
     5581 ctggcgattt tgcgcttgtc atcagtctta gcgcgccttc acagcacttt attaattcct
     5641 tgaacgaata tttactgcca tttcattcac gtctattctt aatttgctgc tttaaaaaca
     5701 agtgattgag caaaattgag gcaaaaatgg actggtatct gaaagtacta aaaaattatg
     5761 tcggtttccg gggtcgtgcg cggcgcaaag agtactggat gtttattctg gtcaacatca
     5821 tctttacgtt cgtgctgggg ctgctggata aaatgttagg ctggcaacgc gctggtggcg
     5881 aaggcatcct gacgacaatc tacggtattc tggtgttttt accgtggtgg gcagttcagt
     5941 tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc actgttgttc ttaatcccgt
     6001 ttatcggctg gcttattatc atcgtcttta actgccaggc gggtacgcca ggcgaaaacc
     6061 gctttggacc agatccgaaa ctggaacctt aaaacaaatc tgctcgccgg atgtctgact
     6121 catccggctt ttatc
//
LOCUS       DAPGWD010000213         6102 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPGWD010000213 DAPGWD010000000
VERSION     DAPGWD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.215"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1099
                     /locus_tag="RHX88_004124"
                     /pseudo
     CDS             1..1099
                     /locus_tag="RHX88_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1068..2804)
                     /gene="aceK"
                     /locus_tag="RHX88_004125"
     CDS             complement(1068..2804)
                     /gene="aceK"
                     /locus_tag="RHX88_004125"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDV2232741.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3085..4389)
                     /gene="aceA"
                     /locus_tag="RHX88_004126"
     CDS             complement(3085..4389)
                     /gene="aceA"
                     /locus_tag="RHX88_004126"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDV2232742.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4419..6020)
                     /gene="aceB"
                     /locus_tag="RHX88_004127"
     CDS             complement(4419..6020)
                     /gene="aceB"
                     /locus_tag="RHX88_004127"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDV2232743.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1497 a         1578 c         1507 g         1520 t
ORIGIN      
        1 ctgcacaata tattaatgat atttatcaga atgttgatta caaaccccat caagatgatt
       61 actcttcagc tgaaaaattt ctcacgcact tcaacaaaaa atgcaaaaac cagactttgg
      121 cgttggtttc cagccgtcct gaggggcgtt acgttgctgc ctgtggtgat ttcgggctag
      181 ttatgaaggc atatttcgac aagatggaat caaatggcat cagtgttatg gcagccatat
      241 tattggtgga taaccatgct ttgacggcgc ggctaagaat aaagaacaca actgaaggat
      301 gtacccatta cgtggtttcg gtttatgatc ctaatgtcac taacgataaa ataagaatta
      361 tgagcgaaag caaagaggat attaaacact attctctgat ggattttatg aatgtagatt
      421 atagcctcct gaaatggtca aatgatcata ttattaacca atctgttgca ataattccag
      481 cacttccgaa agaacagcta ttgatgttaa aaggatctgt ggatgaaata acccctccat
      541 tatcacctgc aacgatgaat ttgctaatgg caattggtca gaatcaccaa cttacgcaac
      601 tgatgattca actccagaaa atgccagaac tacatagaac agaaatgttg actgcctata
      661 atagtggaca tatgaacgtt attaatacta tttttaacgc attacccact ctttttaata
      721 cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt ctaatgaata
      781 tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga cggtttatct
      841 tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta tggatttttg
      901 gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat ttggtcaccg
      961 ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct ttggctttac
     1021 ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca aaaaagcatc
     1081 tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc ttccacatgc
     1141 ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc
     1201 atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg aaactcttcc
     1261 gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc gtcttccgga
     1321 tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa
     1381 tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag catgtcgcca
     1441 ggaaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc aatggcgtcg
     1501 cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat ccgccgctcg
     1561 atatagagat ggcgaattac aatctgctca ccaagatcgg tgattttttc cgctgcttcc
     1621 tgaagcagta attccattaa tgccggggaa atatgccgct tctccagcac aaagttttca
     1681 aactcctggg tgtccgccat tcggcccaca cggtcgtgct ctttcaccag ttgatagcag
     1741 gcacgaacgt gagcggcaga catctctttc tgcggcgcga acttgtcttt gatgactttg
     1801 aatacccgat caaagcccgg cagcgtaaac accaacatca ccattccacg aatacccggc
     1861 gcttcaatga actgctcatt acagccctgt agataaacga gatattcgcg gtagctttcg
     1921 gttttggcgt gcttctggca gccgatagcc atatacaatt cagcagtggt tttacctggc
     1981 agaatttccc gtagccactc gaccagcgct gcgggcagcg gtgcataaac cataaaataa
     2041 gaacgcgcaa agccaaaaac aatgctcgct tcggcggtcg tcgtcaggca ggtatcaata
     2101 aataactcgc cgtcgtccgt ctggtggatc ggcagtaaga agggcaacgt gccggaaggc
     2161 gtgatcagtt tgcccaccag ccaggcggct ttattgcggt aaaacagttc gttcgccacc
     2221 tgtaaatgac tttccgcgag gttgtctgtc cccagcgttt ccgtcagatg gcgaatgatg
     2281 taatgaatgt cacggctttt attctgccag cgcaggcgca gcggcaggtc gctgataacg
     2341 cgcatcagta gagattccca gccgtgatcg gggtgaaagt ctttcgccag cgggcgggga
     2401 atggtacgaa agcggcgctc tggctgagag ctaaagataa aaagccgctc gggagtaagc
     2461 gagcggtggt caaataaccg acagtacacg gagttaaaaa agctctccgc aatctcgaag
     2521 cgcgggtaat ccggcaacag ccgcgtgtaa tgctctttaa cgcgcagtaa aaatgccgcg
     2581 tccgtacttt ggccgttagt aatgcagcgc agttgctcca cgaccagacc aacgtgatga
     2641 tcgtaaagat ggatacggtt tttcatcgcc tgctggacag catgccagtc ggcctgttcg
     2701 aaacgctgct gcgccccgga agtcacttcg aggaatcgac catactgagc gtcgaagcct
     2761 tgcaaaatgg tttgagcaat cagtaattcc aggccacgcg gcatttagcg ccctcatcag
     2821 gagcagagaa ttgccggatg cggcgtaaac gccttatcca gcctacgttc ggcaacggtt
     2881 gtaggcatga taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg
     2941 gcgtaaatgc cttatccggc ctacgttcgg caacggctgt aggcatgata agacgcgcaa
     3001 gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtgaacgcct tatccggcct
     3061 acagtcagca acggttgttg ttgcttagaa ctgcgattct tcagtggagc cggtcagcgc
     3121 ggtgactgaa gacgtgccgc cctgaataat agtcgtcact ttatcgaagt aacctgtacc
     3181 cacttcctgc tggtgagata cgaaggtata gccatctttc gcggcggcaa attccggctg
     3241 ctgcactttc tcaacgtagt gcttcatacc ctcgccctgg gcataggcgt ttgccaggtc
     3301 aaacatgttg aaccacatgc tgtggatacc tgccagggtg atgaactgga acttgtagcc
     3361 catatccgac agctgctgct ggaagctggc aatagttttg tcgtcgaggt ttttctgcca
     3421 gttgaacgac ggcgagcagt tataagccag cagtttgccc ggatatttcg cgtggatagc
     3481 ttgtgcaaag cgacgcgcca gttccagatc cggcgtggag gtttcacacc agaccaggtc
     3541 agcatatggc gcatacgcca ggccacggct gatcgcttgc tcaatgcccg catgagtacg
     3601 gaagaagcct tcactggtac gctcgccggt aataaattcg ctgtcatacg ggtcgcaatc
     3661 ggaggtgatc agatccgctg catcggcatc ggtacgagca accagtaggg ttggaacgcc
     3721 cgtcacgtca gctgccagac gcgccgcgac cagtttctga atagcttctt gagttggcac
     3781 caaaactttg ccgcccatgt gaccgcattt cttcactgac gccagctgat cttcgaagtg
     3841 aactgccgct gcaccggctt caatcatcgc tttcatcagt tcaaaggcat tcaggacacc
     3901 gccaaaaccg gcttccgcat cggcaacgat cggcaggaag taatcgacat agcgcggatc
     3961 gcccggctca atgcccgcgg accattggat ctgatcggca cgacggaagg tgttgttgat
     4021 ccgctccacc acagctggca ccgagtttgc cggatagagc gactgatccg gatacatgct
     4081 ggccgccagg ttagcgtccg ccgctacctg ccatcccgac agatagactg cttcaatacc
     4141 cgctttcgcc tgttgcagcg cctgaccgcc agtcagtgca ccgaggctgt tgatgtagcc
     4201 ttttttcgac tcgccatgca gcagacgcca cattttcgcc gcgcccagtt gcgccagggt
     4261 gcattcagga ttgactgaac cgcgtaattt caccacatct tccgcgctgt atgggcgagt
     4321 aatgccttcc caacgcggtt gagtccactc tttctgtaat tcttcaattt gttgtgtacg
     4381 ggttttcatg tgcagatgct ccatagttat gtggtggttt acgctaacag gcggtagcct
     4441 ggcagggtca ggaaatcaat taactcatcg gaagtggtga tctgttccat caagcgtgcg
     4501 gcatcgtcaa aacgcccctg ggagaaacgt tcttcgccca gttcgctggc aatgactttc
     4561 atctcttcac ccagcatctg gcggaacaag gctttggtca ccggtttgcc attgctcaac
     4621 gttttttgat gatggatcca ctgccagatt gaggtacggg aaatttcagc cgtcgccgca
     4681 tcttccatca ggccataaat cggcacgcag ccgttgccgg agatccatgc ttcgatgtac
     4741 tgcactgcca cgcgaatgtt ggcgcgcata ccttcttcgg tgcgttcacc atcacaaggt
     4801 gccagcagct gatcggcagt aatcggcgcg tcttgttcgc gcatcacttc aagctgattt
     4861 ttacgggagc cgagaatgtc gttgaatacc gccattgccg tatccgcaag gcctgggtga
     4921 gcaatccatg tgccatcgtg accgttattg gcttccaacg ctttatccgc ttttactttg
     4981 ttgagcacct ggttattgcg ctcttcatct ttgctcggaa taaacgctgc catgccgccc
     5041 atcgcaaaag caccgcgttt atggcaggtt ttaatcaaca ggcgtgagta agcattcagg
     5101 aatggtttat ccatcgtcac tgcctgtctg tctggcagga cgcgatcggg atagtttttc
     5161 aacgttttga tatagctgaa gatgtaatcc caacgaccgc agttcagacc aacaatatgg
     5221 tcacgcagcg cgtgaaggat ttcatccatc tggaacacgg cgggcagcgt ttcaatcagc
     5281 aacgtcgcct tgatggtgcc gcgcggcaga ttaaagcgat cttctgcata gctgaagact
     5341 tcgctccacc aggccgcttc ctgccaggac tgggttttcg gcagatagaa atagggaccg
     5401 ctgccctttg ccaacagtgc ctgatagttg tggaagaaat agagcgcaaa atcaaacagg
     5461 ctaccgggga ttgcctcacc acgccaggtg acatgttttt ccggcaagtg cagaccgcgt
     5521 acccgacaaa tcaaaaccgc tggattgggc ttgagctgat aaattttgcc tgcttcattg
     5581 gtatagctga tggtgccgtt aaccgcatcg cgcaggttaa tttgcccgtc gatcactttg
     5641 ttccagtctg gtgccagtga atcttcgaaa tcggccataa agactttcac attggcgttc
     5701 agtgcgttga tcaccatctt gcgctcaacc gggccagtta tctcgactcg acgatcttgt
     5761 aagtccgcgg gaataccacg aattttccag tccgtatcgc gaatggaagc tgtttccgaa
     5821 ataaaatcag gcaaagttcc gttatcaata tcttgctgct gctgaatgcg cgctgccaga
     5881 agtttattgc gttgtggcgt aaaatgcgtc accagctcag tcagaaattc taccgcttcg
     5941 gcagtaagaa tttgcttctc ctgctcgcca tacggcctta tgaaagccag ttcatcggtt
     6001 gttgttgcct gttcagtcat cgtgcagctc ctcgtcatgg atccgaaaac ttccccactg
     6061 tgaacgaagg atcgttgtgc gcttttcgtt cagcacaact aa
//
LOCUS       DAPGWD010000214         5806 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPGWD010000214 DAPGWD010000000
VERSION     DAPGWD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.216"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(149..460)
                     /locus_tag="RHX88_004128"
     CDS             complement(149..460)
                     /locus_tag="RHX88_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV2232744.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            659..1357
                     /locus_tag="RHX88_004129"
     CDS             659..1357
                     /locus_tag="RHX88_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2232745.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1402..2301)
                     /gene="eamA"
                     /locus_tag="RHX88_004130"
     CDS             complement(1402..2301)
                     /gene="eamA"
                     /locus_tag="RHX88_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDV2232746.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2496..3683
                     /gene="ydeE"
                     /locus_tag="RHX88_004131"
                     /pseudo
     CDS             2496..3683
                     /gene="ydeE"
                     /locus_tag="RHX88_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            3810..3905
                     /gene="mgtS"
                     /locus_tag="RHX88_004132"
     CDS             3810..3905
                     /gene="mgtS"
                     /locus_tag="RHX88_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDV2232747.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(4124..5014)
                     /gene="dgcZ"
                     /locus_tag="RHX88_004133"
     CDS             complement(4124..5014)
                     /gene="dgcZ"
                     /locus_tag="RHX88_004133"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDV2232748.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLYVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(5269..5661)
                     /gene="ydeI"
                     /locus_tag="RHX88_004134"
     CDS             complement(5269..5661)
                     /gene="ydeI"
                     /locus_tag="RHX88_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDV2232749.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1605 a         1374 c         1198 g         1629 t
ORIGIN      
        1 ccattccacg ttcgaatgag gccattaatc ccattttttt atacctgttg ttttggatat
       61 ttatacatcc caataaagca tgtatgccat atccatttta tgatgtgtga aaagttagtt
      121 caggtaatag tttaataata ttagattctt actcttgcag tagtttcatc gcaaattcaa
      181 gaactgcctg tccgttcatg gtgccgtagt ctttcatctc gataacggct acaggcattt
      241 tcccctgtgc aacggcttca atctctggtt tctgaaagcg aacttgcggg ccaagtaaaa
      301 caacgtcaac ttcgttattt ttaattttcc ctttagcctc tgcaatcgcc aatgcggaaa
      361 tattaacttc aagtgaaata gcgttagcat gatcaatcat acgtttaacc agcatactgg
      421 ttgacatgcc cgcagcgcaa actaacaata tttttttcat attttccctt actggtatat
      481 aacagattac attgtttaaa tatcatatcc cgcagtagct atacgtcctg gtacattgat
      541 cacagtaata cagtgataaa gttgttcaac atcagggtga ttattaaacg actcggccat
      601 acgataaacc aatcaggact caactccttg ttttcaatgg ctcttaaaag gtgcaaaagt
      661 gatttttcaa aagattgccc gcttacttaa atccgaaatc aatggcaatt catggcatgt
      721 tggtgatttg ttgccgtcag aagcggaact cgctgttcgt tataatgttt cgcgtaatac
      781 cctgcgtaag gcattatcct tgctggaagg cgaaggaatt attcacagaa agcatggttc
      841 aggaacatac attcaaaaaa agaattttgt tgcacacatt gatcacatga acagtttcag
      901 tgaaattgcg cataaaagcg gcaaagaggc agggagtcag attatgaaat ttgaagtgca
      961 ggatgcttca cccactattg cgactgagct gaatttagtg actggtgagc aggtttatta
     1021 cataaagaga ctgcgattca ttgaggataa tgcagcacaa ttagaagaaa cgtggatgtc
     1081 agtagcacgt tttcctgatt taaccgtgtc acatatgcaa aaatccaaat tttcatatat
     1141 tgagaatgaa tgcgggatca aaatcattgg cacctttgaa actttctccc ctacttttcc
     1201 taccccagaa atcgccagta ttttacggat cagcccacgg gatcccatac ttaaaattca
     1261 gacccaggct gtggacagta actctattcc gctggattat tcgttacttt acagcaatat
     1321 tttcgagttc caggtaaagt actttttccc gcgataaaag cagacgcaca gagtgctgac
     1381 atcacattct gtgcgtatgg tttagcctct aacctttacc gctttacgcc accgcaagcc
     1441 aaatacattg atatacagcc cggtcataat gagcaccgca cctaaaaatt gcagacccgt
     1501 taagcgttca tccaacaata gtgccgcact tgccagtccg actacgggca ccagtaacga
     1561 taacggtgca acccgccagg tttcatagcg tcccagtaac gtcccccaga tcccataacc
     1621 aacaattgtc gccacaaacg ccagatacat cagcgacaag atggtggtca tatcaatagt
     1681 aaccagactg tgaatcatgg atgcggaacc atcgagaatc agcgaggcaa caaagaaggg
     1741 aatgattggg attaaagcgc tccagattac cagcgacatc accgccggac gcgttgagtg
     1801 cgacatgatc tttttattga agatgttgcc acacgcccaa ctaaatgctg cagccagggt
     1861 caacataaag ccgagcatcg ccacatgctg accgttcaga ctatcttcga ttaacaccag
     1921 tacgccaaaa atcgctaagg cgatccccgc caattgtttg ccatgcagtc gctccccgaa
     1981 agtaaacgcg ccaagcacga tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag
     2041 tccagcaggc ataccgaagt taatggcaca aaaaagaaaa gcaaactgcg caaaactgat
     2101 ggttaatcca taccccagca gcaaattcag tggtactttc ggtcgtgcga caaaaaagat
     2161 agccggaaaa gcgaccagca taaagcgcaa accggccagc atcagcggtg gcatgttatg
     2221 aagccccact ttgatgacca caaaatttag cccccatacg accactacca gtagcgccaa
     2281 caccccatct tttcgcgaca ttctaccgcc tctgaatttc atcttttgta agcaatcaac
     2341 ttagctgaat ttacttttct ttaacagttg attcgttagt cgccggttac gacggcatta
     2401 atgcgcaaat aagtcgctat acttcggatt tttgccatgc tatttcttta catctctaaa
     2461 acaaaacata acgaaacgca ctgccggaca gacaaatgaa cttatcccta cgacgctcta
     2521 ccagcgccct tcttgcctcg tcgttgttat taaccatcgg acgcggcgct acgctgccat
     2581 ttatgaccat ttacttgagt cgccagtaca gcctgagtgt cgatctaatc ggttatgcga
     2641 tgacaattgc gctcactatt ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata
     2701 agttcgacaa gaaacgctat atgttactgg caattaccgc cttcgccagc ggttttattg
     2761 ccattccttt agtgaataac gtaacgctgg tggtgctctt ttttgccctc attaactgcg
     2821 cctattctgt ttttgctacc gtgctgaaag cctggtttgc cgacaatctt tcgtccacca
     2881 gcaaaacgaa aatcttctca atcaactaca ccatgctaaa cattggctgg accatcggtc
     2941 cgccgctcgg cacgctgttg gtgatgcaga gcatcaatct gcccttctgg ctggcagcta
     3001 tctgttccgc gtttcccatg cttttcattc aaatttgggt aaagcgcagc gagaaaatca
     3061 tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt attacaagat aaagcactgt
     3121 tgtggtttac ctgctctggt tttctggctt cttttgtaag cggcgcattt gcttcatgca
     3181 tttcacaata tgtgatggtg attgctgatg gggattttgc cgaaaaggtg gtcgcggttg
     3241 ttcttccggt gaatgctgcc atggtggtta cgttgcaata ttccgtgggc cgccgactta
     3301 acccggctaa catccgcgcg ctgatgacag caggcaccct atgtttcgtc atcggtctgg
     3361 tcggttttat tttttccggc aacagcctgc tattgtaggg tatgtcagct gcggtattta
     3421 ctgtcggtga aatcatttat gcgccgggcg agtatatgtt gattgaccat attgcgccgc
     3481 caggaatgaa agccagctat ttttccgccc agtctttagg ctggcttggt gccgcgatta
     3541 acccattagt gagtggcgta gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct
     3601 tagcgttggt gatcattgct gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt
     3661 gggggcagcc cgcgctttgt tgatttaagt cgaacacaat aaaaatttaa ttcagccttc
     3721 gtttaggtta cctctgctaa tatctttctc attgagatga aaattaaggt aagcgaggaa
     3781 acacaccaca ccataaacgg aggcaaataa tgctgggtaa tatgaatgtt tttatggccg
     3841 tactgggaat aattttattt tctggttttc tggccgcgta tttcagccac aaatgggatg
     3901 actaatgaac ggagataatc cctcacctaa ccggcccctt gttacagttg tgtacaaggg
     3961 gcctgatttt tatgacggcg aaaaaaaacc gccagtaaac cggcggtgaa tgcttgcatg
     4021 gatagatttg tgttttgctt ttacgctaac aggcattttc ctgcactgat aacggatcgt
     4081 tgacacagta gcatcagttt tctcaatgaa tgttaaacgg agcttaaact cggttaatca
     4141 cattttgttc gtcaataaac atgcagcgat ttcttccggt ttgcttaccc tcatacattg
     4201 cccggtctgc tcttccaatg accacataca gaggctcttc aggaaatgcg cgactcacac
     4261 ctgctgtcac ggtaatgttg atatgccctt cagaatgtgt gatggcatgg ttatcgacta
     4321 actggcaaat tctgacacct gcacgacatg cttcttcatc attagccgct ttgacaataa
     4381 tgataaattc ttcgcccccg tagcgataaa ccgtttcgta atcacgcgtc caactggcta
     4441 agtaagttgc cagggtgcgt aatactacat cgccgattaa atgcccgtag gtatcattaa
     4501 ccaatttaaa tcggtcaata tccaacaaca ttaaataaag attcagaggc tcagtgttgc
     4561 gtaactgatg atcaaaggat tcatcaagaa cccgacgccc cggcaatccc gtcaaaacat
     4621 ccatattgct acggatcgtc agcaaataaa ttttgtaatc ggttaatgcc gcagtaaaag
     4681 aaagcaaccc ctcctgaaag gcgtcgaaat gcgcgtcctg ccagtgattt tcaacaatag
     4741 ccagcattaa ttcccgacca cagttatgca tatgttgatg ggcagaatcc attagccgaa
     4801 cgtaaggtaa ttcatcgtta tcgagtggcc ccagatgatc aatccaccga ccaaactggc
     4861 acagtccata agaatggtta tccgttattt ctggcttact ggcatctctc gcgaccacgc
     4921 tgtgaaacat actcaccagc cactggtagt gggcatcgat agccttattg agatttaaca
     4981 agatggcatc aatttccgtt gtcttcttga tcattgccac tcctttttca cagttccttg
     5041 tgcgcgctat tctaacgaga gaaaagcaaa attacgtcaa tattttcata gaaatccgaa
     5101 gttaggagtc atctctgaga taacattgtg atttaaaaca aaatcagcgg ataaaaaagt
     5161 gtttaattct gtaaatcacc tctgcattat cgtaaataaa aggatgacaa atagcataac
     5221 ccaataccct aatggcccag tagttcaggc catcaggcta atttattttt atttctgcaa
     5281 atgagtgacc cgaacgacgg ccggcgcgct tttcttatcc agactgccgc taatgttgat
     5341 catctggtcc ggctgaactt ctcgtccatc aaagacggcc gcaggaataa cgacattaat
     5401 ttcaccgctc ttatcgcgaa aaacgtaacg gtcctctcct ttgtgagaaa tcaaattacc
     5461 gcgtagtgaa accgaagcgc catcgtgcat gttttttgcg aaatcaacgg tcattttttt
     5521 tgcatcatcg gttccgcgat agccatcttc tattgcatga ggcggcggtg gcgctgcatc
     5581 ctgttttaaa ccgccctggt catctgccaa cgcataaggc atgacaagaa aacttgctaa
     5641 tacaatggcc tgaaatttca tactaactcc ttaattgcgt ttggtttgac ttattaagtc
     5701 tggttgctat ttttataatt gccaaataag aatactgcca attgttataa ggcatttaaa
     5761 atcagtccac tagctgtcaa atattcagag aatttaactc actaaa
//
LOCUS       DAPGWD010000215         5786 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPGWD010000215 DAPGWD010000000
VERSION     DAPGWD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.217"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            13..459
                     /locus_tag="RHX88_004135"
     CDS             13..459
                     /locus_tag="RHX88_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDV2232750.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            470..1921
                     /locus_tag="RHX88_004136"
     CDS             470..1921
                     /locus_tag="RHX88_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDV2232751.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            1911..2981
                     /locus_tag="RHX88_004137"
     CDS             1911..2981
                     /locus_tag="RHX88_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV2232752.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRNILINTLESCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIANIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            2981..4729
                     /locus_tag="RHX88_004138"
     CDS             2981..4729
                     /locus_tag="RHX88_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDV2232753.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDIWPQYPGNEIKQIV
                     EQCGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4779..>5786)
                     /locus_tag="RHX88_004139"
     CDS             complement(4779..>5786)
                     /locus_tag="RHX88_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDV2232754.1"
                     /translation="VAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKP
                     ETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTGEERTVY
                     GIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQE
                     NYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAELKNIEEREGKTF
                     HYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAKEADKGGMNFSFINSA
                     GQYQLEAKKYVRRVRDKVPYSDWDKEQLQDANSSWMVDDSFPRALREYNEMVDDYNSL
                     R"
BASE COUNT         1330 a         1486 c         1490 g         1480 t
ORIGIN      
        1 ggagtccgat taatggcagc tcttactgca agctgtattg acctgaatat tcagggcaat
       61 ggcgcttatt ccgttctgaa gcagttggcg acaatagcgt tacaaaacgg ttttatcacc
      121 gactcacacc agttcctgca aaccctgctg ctgcgcgaaa aaatgcactc tactggattt
      181 ggttccggtg tcgccgtgcc gcacggtaaa agcgcctgcg ttaaacaacc gttcgtatta
      241 ttcgcccgca aagcgcaggc tattgactgg aaagccagcg atggcgaaga cgtcaattgc
      301 tggatctgcc tcggcgtgcc gcaaagcggc gaagaggatc aggtcaaaat catcggcaca
      361 ctgtgtcgca aaattatcca ccaggatttt attcatcaac tgcaacaggg cgacgccgac
      421 caggtgcttg ccttgttaaa tcaaaccctc agctcataag gaagtggcga tggagtcatc
      481 cttacgtatt gtcgcgatca ccaactgccc cgccgggatc gctcacacct acatggtggc
      541 ggaagccctg gaacagaaag cccgttctct cggccatacc ataaaagtgg aaactcaagg
      601 gtccagtggc gttgaaaacc gcttatccag cgaagagatt gccgctgccg attacgtcat
      661 tctcgctacc gggcgtggcc tgagcggtga tgatcgcgcg cggtttgccg ggaagaaagt
      721 ttatgagatt gccatctccc aggcgttgaa aaatatcgac cagattttca gcgaattacc
      781 gacaaactcg cagctttttg ccgcagatag cggcgtgaag cttggcaaac aggaagtgca
      841 aagcggcagc gtgatgagcc acctgatggc gggcgtctct gcggcgctgc cgtttgtcat
      901 cggcggcggt attctggtgg cgcttgccaa catgctggtg cagttcggtt tgccatatac
      961 cgatatgtcg aaaggtgcgc cgtcgtttac ctgggtagtt gaatccatcg gttacctcgg
     1021 cttcaccttt atgatcccca tcatgggcgc ttatatcgcc tcgtcgattg ccgataaacc
     1081 ggctttcgct ccggcgtttc tggtttgcta tctggcgaac gacaaagccc tgctcggcac
     1141 ccagtcgggt gcgggattcc tcggcgcggt ggtgctgggg ctggcgattg gctatttcgt
     1201 cttctggttc cgtaaagtgc gccttggcaa agcattacaa ccgctgctcg gttccatgct
     1261 gatcccgttc gtcactctgc tggttttcgg cgtgctcact tactacgtta tcggaccggt
     1321 gatgtccgac ctcatgggcg ggctgctgca cttcctgaac accattcctc catcaatgaa
     1381 gtttgcggca gcgtttctgg tcggtgcgat gctggcgttc gatatgggcg gccccatcaa
     1441 taaaaccgcc tggttcttct gcttctcact gctggaaaaa cacatttatg actggtacgc
     1501 catcgtcggc gttgtcgcgc tgatgccgcc cgtcgcggcg ggtcttgcga cgttcattgc
     1561 gcccaaactg tttacccgac aggaaaaaga ggctgccagt agtgccattg tggtcggcgc
     1621 cacagtcgca accgaaccgg ctattcctta cgctctggca gctccgttgc cgatgattac
     1681 cgccaatacg ctggcgggcg gcattaccgg cgtgctggtg atcgctttcg gaatcaaacg
     1741 tctggcacct ggtttgggta tctttgaccc gctgatcggc ctgatgtcgc cggtaggttc
     1801 gttttatctg gtgctggcga ttggcctcgc gctgaacatt tcattcatta tcgtcctgaa
     1861 aggattgtgg ttacgccgca aagcgaaagc cgcgcaacag gagcttgtcc atgaacattg
     1921 agttactgca acagttgtgc gaagccagcg ccgttagcgg cgatgaacag gaagttcgca
     1981 acatcctgat aaacacgctg gaatcctgcg tgaatgaaat cacctttgat ggtctgggca
     2041 gctttgttgc ccgtaagggg aataaaggtc caaaagttgc cgttgtcggg catatggatg
     2101 aagtcggctt tatggtcacc cacatcgacg agagcggttt tctgcgcttt accaccattg
     2161 gcggctggtg gaatcagtcg atgctcaacc accgggtaac catacgcaca cacaagggat
     2221 ttaaaatccc cggtgtgatt ggttccgtcg cgccacatgc gttaacggaa aagcaaaagc
     2281 aacaaccgct gtcatttgat gagatgttca ttgatattgg cgcgaacagt cgcgaagaag
     2341 cggaaaagcg cggcgttgaa attggcgatt ttattagccc ggaagccaat tttgcctgct
     2401 ggggcgaaga taaagtagtc ggcaaggcgc tggataatcg catcggctgc gcgatgatgg
     2461 cggagctact acagacagta aataaccccg aaattacgct gtacggcgtc ggcagcgtgg
     2521 aagaagaagt tgggctacgc ggggcacaaa cctcggcgga acacattaaa ccggatgtgg
     2581 tgattgtgct ggataccgct gtcgcaggtg atgttccggg cattgctaac attaaatacc
     2641 cgctgaaact gggccagggg ccggggctga tgctgtttga caagcgctac ttccccaacc
     2701 agaaactggt agcagcgtta aaaaactgtg ccgcacataa cgatttaccg ctgcaatttt
     2761 ccaccatgaa aaccggtgcg acggatggcg ggcgctacaa cgtgatgggc ggcgggcgtc
     2821 cggttgtcgc gctgtgtctg ccaactcgtt atctgcacgc caacagcggt atgatttcaa
     2881 aagccgatta cgatgctctg ctcacgctga tacggggttt tctgacgacc ttaactgcgg
     2941 agaaagtcaa cgcgtttagc cagttccgtc aggtggatta atgctaaacg agcgccagtt
     3001 aaagattgtc gatctgctgg agcaacagcc gcgcacgcct ggtgagctgg cgcaacagac
     3061 tggcgtttct ggcaggacca tactgcgtga tattgactat ctcaacttta cccttaacgg
     3121 caaagcccgc atttctgcca gtggtagtgc gggctatcag ctggaaatct tcgagcgccg
     3181 cagctttttt cagttgctgc aaaagcacga taacgacgat cggctgctgg cgctattatt
     3241 actaaatacc tttactcccc gtgcgcaact cgcctcggcg cttaatttgc cagaaacgtg
     3301 ggtagcagaa cgtctgcccc ggttaaaaca gcgttatgaa cgcacttgtt gcctggccag
     3361 tcgccccggt ttgggccatt tcattgatga gacagaagag aaacgcgtta tcttgctggc
     3421 gaacttgcta cgcaaagatc cgtttttaat tccgctggcg ggcataacac gagacaacct
     3481 tcagcattta tccacggcct gcgacaacca acaccgctgg ccgctgatgc agggtgatta
     3541 tctctccagc ctgattctgg cgatttacgc cctgcgtaat cagcttactg acatatggcc
     3601 gcaatatccc ggtaacgaga taaaacaaat cgttgaacag tgcggcctgt ttctcggtga
     3661 taacgctgta agaaccctga cgggtttgat agagaaacag catcagcaag cgcaggtaat
     3721 ttcagccgat catgtgctgg ggttgctgca aagggtgcca ggcatcgcgt cattgaatat
     3781 tattgatacg cagttggttg agaatattac cgggcattta ttacgttgcc tcgccgcacc
     3841 agtgtggatt gctgagcacc gccaaagcag catgaataac ctgaaagccg cctggcctgc
     3901 ggcctttgat atgagtctgc actttattac gctactgcgt gaacagctcg atattcccct
     3961 tttcgacagc gatctgatcg gtttgtattt tgcctgtgcg ctggagcggc atcaaaacga
     4021 acgccagccg atcattttgc tctcggacca gaacgcgatt gccactatta atcagctcgc
     4081 cattgagcgc gatgttttaa attgtcgggt aattattgcc cgtagcttaa gcgaacttgt
     4141 tgccattcgc gaagagattg agccgttatt gatcattaac aatagccatt atttactgga
     4201 agacgcggta aataattaca tcaccgtaaa aaatatcatt acggctgccg gtatcgaaca
     4261 aataaaacat tttctggcga cggcatttat tcgccagcaa ccggagcgtt ttttctctgc
     4321 ccccggaagt tttcattatt cgaatgtacg cggtgaaagc tggcaacata ttacccggca
     4381 aatttgtgcg caattagtgg cacaacacca tattaccgcc gatgaagcac aacgcatcat
     4441 cgcccgcgaa ggcgaaggtg aaaacctgat tgttaatcgc ctcgccatcc cacattgctg
     4501 gagcgaacag gagcgacgtt ttcgtggatt ttttattacc ctcgcccaac cagttgaggt
     4561 gaataacgaa gtcattaacc atgtcttgat cgcctgcgcc gccgccgatg cgcgtcatga
     4621 gctaaaaata tttagctatc tggcaagcgt attgtgtcag catccggcag aggttattgc
     4681 cgggttaaca ggatatgagg catttatgga gttacttcac aaggggtgaa ctgtgtcgga
     4741 tgcgccgcat ccgacactgc atttatcatc aaaaacgttt aacgcaggct attatagtca
     4801 tcaaccattt cgttgtactc gcgtaatgct ctcgggaaag agtcgtcgac catccagctt
     4861 gagtttgcgt cctgcagttg ctctttatcc cagtcagagt acgggacttt atctctgacg
     4921 cggcgaacgt attttttagc ctcgagttga tactggcctg ccgaattaat aaacgagaaa
     4981 ttcatgccgc ctttatccgc ttctttggcc tgcgccacca gcgtttccag ttcagacact
     5041 ttcttcattg ccgcttctgc atcaaactta tcttgcgata tcaggttatt aatttgtttc
     5101 gctgaaatca tgactgccag agagtaatag tggaatgttt tcccttctct ttcttcaata
     5161 tttttcagtt ccgcaagctg gcgcttatcg ttaatttcct gaatcgccgc atgataagat
     5221 tctgctacag cttcaaaggc ttccagattt tttaagaatg tctggtgcag cgttttccct
     5281 ttcgcaaacg catcgtcttt ataattttcc tgggtgtaat atttatccat ttcgttaatg
     5341 gtattaccca acgccacggc agcatcgata taactcaccg ccacgccatc aataggttgc
     5401 agcgcaggcg ttaacgccac agcacttttt acgccttttt cacactcagc gaggttggat
     5461 tcactaatgc cgtagatgcc ataaacagta cgctcttcac cggtaggccc ctgtttaaaa
     5521 tctttcagcc agtcagcata acgcgccaga ctgctctgta cagggatctg taacttgtta
     5581 tagcacttga tatagacatc cattttttcg tcgaaaaccg cttgttcatc gaccaccggt
     5641 tgcgcaggtg tttcaggttt agcttctgct tttgcaacgg gagcttcagg agctggtgcg
     5701 gcaggttgac tattggcagg cgggggcgtt tctgtttcgg cttttttatc atcacaaccc
     5761 gtcagaccga gggccatgat ggcgac
//
LOCUS       DAPGWD010000216         5783 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPGWD010000216 DAPGWD010000000
VERSION     DAPGWD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.218"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            16..1005
                     /gene="ldhA"
                     /locus_tag="RHX88_004140"
     CDS             16..1005
                     /gene="ldhA"
                     /locus_tag="RHX88_004140"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV2232755.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1116..1538
                     /gene="hslJ"
                     /locus_tag="RHX88_004141"
     CDS             1116..1538
                     /gene="hslJ"
                     /locus_tag="RHX88_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDV2232756.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTNDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1535..1801)
                     /locus_tag="RHX88_004142"
     CDS             complement(1535..1801)
                     /locus_tag="RHX88_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDV2232757.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2075..5599
                     /gene="nifJ"
                     /locus_tag="RHX88_004143"
     CDS             2075..5599
                     /gene="nifJ"
                     /locus_tag="RHX88_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDV2232758.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1453 a         1482 c         1490 g         1358 t
ORIGIN      
        1 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
       61 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
      121 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
      181 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
      241 tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag aactggggct gaaagtagtc
      301 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
      361 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
      421 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
      481 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
      541 tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
      601 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
      661 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
      721 gcattgattg attctcaggc agcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
      781 ggtatggacg tgtatgagaa cgaacgcgat ctattctttg aagataaatc caacgacgta
      841 attcaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctatt taccgggcac
      901 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
      961 agcaatctgg aaaaaggcga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     1021 gcattccagg ggagctgatt cagataatcc ccaatgacct ttcatcctct attcttaaaa
     1081 tagccctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgctcgttg
     1141 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgta acgccagaac
     1201 agttacagca tcatcgtttt gtgctggaaa gcgtaaacgg taagcccgtg accaacgata
     1261 aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc atgtgtaacc
     1321 gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg ctggcaatga
     1381 cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc
     1441 tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac
     1501 agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg
     1561 caagtgactg ttcactacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac
     1621 catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac
     1681 tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag
     1741 gttcactact acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca
     1801 tcttttcttc ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga
     1861 gggcgacgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt
     1921 ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa
     1981 atgcagaaga aagccatttg ctaaaattga aggattacta ctgggcgcgc agcaatttcg
     2041 tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc
     2101 ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta ttacccccag
     2161 ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttaaaga acgtttgggg
     2221 agacacacca cgcgtagttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg
     2281 cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt tgctgctgat
     2341 gatcccgacg ctgtacaaac tggcaggcga actgacgcca tttgtcctgc atgtagcggc
     2401 acgtaccgtt gccacacatg cactttctat ttttggcgat cattccgacg ttatggcggt
     2461 gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc caggaagcgc aagattttgc
     2521 tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca tttattcatt tctttgatgg
     2581 tttccgcacg tcccacgaaa tcaataaaat tgtcccgctg gccgatgaca ctattcttga
     2641 tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcgctcaacc cggaacatcc
     2701 ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg aagccaccaa
     2761 cccatggtac gacgcggtct atgaccatgt tgaacaggcg atgaatgatt tctctgccgc
     2821 gacaggtcgt cagtatcagc cgtttgaata ttacgggcat ccgcaagcgg aacgggtgat
     2881 tatcctgatg ggctctgcca ttggcacctg tgaagaagtg gttgatgaat tgctaacccg
     2941 tggcgaaaaa gttggcgtgc tgaaagttcg cctgtaccgt cccttctccg ccaaacactt
     3001 gctgcaagct ctgccgggat ccgtacgcag cgtggcggta ctggacagaa ccaaagaacc
     3061 cggtgcccag gcagaaccgc tctatctgga tgtaatgacc gcactggcag aagcctttaa
     3121 taatggcgag cgcgaaactc tgccccgtgt cattggtggg cgctatggtc tttcatccaa
     3181 agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa
     3241 agcgcgcttt acggttggta tttacgatga tgtgaccaat ctgtcactgc cgttgccgga
     3301 aaacaccctg ccaaactcgg cgaaactgga agccttgttt tatggccttg gtagtgatgg
     3361 cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt aattccacgc cgtggtacgc
     3421 acagggctat tttgtttacg actccaaaaa ggcgggcggc ctgacggttt ctcaccttcg
     3481 cgtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg
     3541 ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac ctggcggcat
     3601 tttcctgctc aacacgccgt acagcgcaga tgaagtgtgg tcgcgcttgc cgcaggaagt
     3661 tcaggccgtg ctaaatcaga aaaaagcgcg cttctatgtg attaacgcgg cgaaaatcgc
     3721 ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct
     3781 gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga ttgccaaaag
     3841 ttacagtagc aaaggccagg atctggtgga acgcaactgg caggctctgg cgctggcgcg
     3901 tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca atcgaccgcc
     3961 agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta accgcagcga tgctcgcagg
     4021 gcttggtgat gccctccccg tttcggcgct gccgccagac ggcacctggc cgatgggcac
     4081 cactcgctgg gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg
     4141 tacccaatgt aaccactgcg tcgccgcttg cccacactca gctattcgcg caaaagtggt
     4201 accgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg
     4261 tgatatgcgc gggcagaaat atgtcttgca ggtggctccg gaagattgca ccggttgtaa
     4321 cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa
     4381 tatgatgtcg cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa
     4441 cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat
     4501 tacaccgctg tttgaatatt caggtgcttg ctccggttgt ggcgagacgc cgtatattaa
     4561 attgctgact cagctctatg gcgaccggat gttgatcgct aacgccaccg gctgttcttc
     4621 aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg gtcgtgggcc
     4681 ggcatgggcg aactctctgt ttgaagataa tgccgaattt ggccttggtt tccgcctgac
     4741 ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg ataaaatccc
     4801 ggcggaatta ctgacagcgt tgaaatcaga cgctacgcca gaggttcgtc gagaacaggt
     4861 cgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc tgcgtgacgc
     4921 agatgcactg gtggaaaaat caatctggct gattggtggt gatggctggg cttacgatat
     4981 cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct
     5041 ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac cgctgggtgc
     5101 agtaactaaa tttggcgagc acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat
     5161 gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc tcaaccagac
     5221 ggtgaaagcg attcaggaag cggaagcgta tccggggcca tcgctgatca tcgcttatag
     5281 cccatgtgaa gagcatggtt acgatctggc actcagccac gaccagatgc gccaactcac
     5341 agctaccggc ttctggccgc tatatcgctt cgatccgcgt cgtgccgatg aaggcaaact
     5401 gccgctggcg ctggattcac gcccgccgtc agtagcactg gaagaaacgt tacttcacga
     5461 gcaacgtttc cgtcggctga attcgcagca gccagaagtg gcagaacagt tatggaaaga
     5521 tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga
     5581 aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa aaaaagcccg
     5641 gacgactgtt cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca
     5701 acagaaaata aaatctgtga caataaaggc atataacccg cgcagaataa cgtatagaaa
     5761 attaaaagta taatttttat ttt
//
LOCUS       DAPGWD010000217         5734 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPGWD010000217 DAPGWD010000000
VERSION     DAPGWD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.219"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..737
                     /locus_tag="RHX88_004144"
                     /pseudo
     CDS             1..737
                     /locus_tag="RHX88_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEc9 family transposase"
     gene            complement(909..2117)
                     /locus_tag="RHX88_004145"
     CDS             complement(909..2117)
                     /locus_tag="RHX88_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDV2232759.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNAR
                     DISLLRYMPA"
     gene            complement(2098..2370)
                     /locus_tag="RHX88_004146"
     CDS             complement(2098..2370)
                     /locus_tag="RHX88_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232760.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            2448..2615
                     /locus_tag="RHX88_004147"
                     /pseudo
     CDS             2448..2615
                     /locus_tag="RHX88_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
     gene            2939..4084
                     /locus_tag="RHX88_004148"
     CDS             2939..4084
                     /locus_tag="RHX88_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV2232761.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            4178..4711
                     /locus_tag="RHX88_004149"
     CDS             4178..4711
                     /locus_tag="RHX88_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV2232762.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(4708..5025)
                     /gene="sugE"
                     /locus_tag="RHX88_004150"
     CDS             complement(4708..5025)
                     /gene="sugE"
                     /locus_tag="RHX88_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV2232763.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
     gene            5077..5547
                     /locus_tag="RHX88_004151"
                     /pseudo
     CDS             5077..5547
                     /locus_tag="RHX88_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1520 a         1411 c         1381 g         1422 t
ORIGIN      
        1 aaagtaaatt gacatccatt acgattgata ttgatagtag tgtaattaac gtagaaggtc
       61 atcaagaagg tgcgtcaaaa ggatataatc ctaagaaact gggaaaccga tgctacaata
      121 tccaatttgc attttgcgac gaattaaaag catatgttac cggatttgta agaagtggca
      181 atacttacac tgcaaacggt gctgcggaaa tgatcaaaga aattgttgct aacatcaaat
      241 cagacgattt agaaatttta tttcgaatgg atagtggcta ctttgatgaa aaaattatcg
      301 aaacgataga atctcttgga tgcaaatatt taattaaagc caaaagttat tctacactca
      361 cctcacaagc aacgaattca tcaattgtat tcgttaaagg agaagaaggt agagaaacta
      421 cagaactgta tacaaaatta gttaaatggg aaaaagacag aagatttgtc gtatctcgcg
      481 tactgaaacc agaaaaagaa agagcacaat tatcactttt agaaggttcc gaatacgact
      541 actttttctt tgtaacaaat actaccttgc tttctgaaaa agtagttata tactatgaaa
      601 agcgtggtaa tgctgaaaac tatatcaaag aagccaaata cgacatggcg gtgggtcatc
      661 tcttgctaaa gtcattttgg gcgaatgaag ccgtgtttca aatgatgatg ctttcatata
      721 acctattttt gttgttcacg tataggaaat tgaaaaacca gacgataccg ctgtccacgc
      781 cttatgccac gtgagccttt ctgcttttcc ctccactccg gtggtttctc tctctgcatg
      841 gtcgattttt ataccgtaac aggctgtttt ttatacctgc accgattccc gggcacactt
      901 agcctgtgtc aggccggcat atacctcagc agactgatat cccgcgcgtt tttcttcagc
      961 cctgatacat tcagccccgc aatggcccgg cggtatacca tccggccgcc acacagcacg
     1021 cattcgaacg ggtcacgact caggaactgt ttcaccattt gtgcatagca cactttcgcc
     1081 actggttccg gcttatccat ccccagagca cggtacacct gcggcagctt ctctccacac
     1141 acccggttgg caaggaaccc gaagtatctc accatcttaa aaaacttctc cgggatgtgc
     1201 tgtttcagcc tcgcgaccag ctcccgctgt gtcagcgttt ccgtcgccgt ttctcccgtt
     1261 ttgtggtcca ggtaacggaa gctcagactc gctcctccgt tgtaatgcgc cagccgggaa
     1321 gccgctattg gtggcttctt cagataacga cccaggtagc gtgccgtatt ccgccctccg
     1381 gctgtcttct tcgacatgta cacatgccag tattttccgc cggctttcag caccaggctt
     1441 ctccactgtg attccgtcgt gatatgtgac aacgattccg gcatcgccag accttctgac
     1501 cacgctttca gaagcagctg ccgcatattc cacatccacc gcgaacgcat cgcgtctttc
     1561 aggaagctca gctttttcca gtgtccgtgt ttattcagac ctccgcaggt cactgacaca
     1621 tgcacatgcg gatgccagtt gagacgacgg ccatacgtat ggatggcgca gaagataccg
     1681 ggttccagac cccgtttccg ggctgcatac agcagattct ccaccgccag acggcacacg
     1741 tcattcagca gccagcggtt gctttcgaac accggccaca gcgtgtccgg cagggtgaag
     1801 accaggtgca cccagtcgca gtcaggaaga cgattcagct gtgtcgctat ccacaggtct
     1861 gtggcctttt ttccgcagga cgggcaggca cggctgccac atgagttagt gacgtatctg
     1921 acgtgggggc actcaggttt atcgcagttg tattccttca cacccagtat ccgcgtgccg
     1981 caggccagca tttttgtgac agcctcaacc tcgatatcgc gcaggccgcc cgcatccagg
     2041 aaggatgtcc agcactggtt ggctgtgaac aggcgtttca gcggtcgggg ctgaaaatca
     2101 ccaccacttc gggtcatccg ccttcatctc cgcttcagtt tcgtataaat caaaacgacg
     2161 gcaggtatgg cagaacgtga cgtattcgta cggtgaatcc gggtacattt tgtggaaggt
     2221 ttcccagaaa cagaccgtac aggagggatg accggcgata aagtctgtaa atacagactg
     2281 ataagggtga ttattggccc tggcgacggc tttcagaacc tctttcacca ttctggtgtg
     2341 gactttctgg tgctccaggt tgtgtgacat gggaactcat tctggatggt tactctgaaa
     2401 gctcatattc tgccacacca cgatttgcag ccgccaggct gccgtggttc aagtttgatt
     2461 ccttgggctc ttcagaatac agacagcaaa taaagacctt tcgtttgaag tatgtatttc
     2521 ttgcagcaaa aataatcaaa accgcaagat atgtaatcat gaagttgtcg gaaaactatc
     2581 cgtacaaggg agtgtatgaa aaatgtctgg tataataaga atatcatcaa taaaattgag
     2641 tgttgctctg tggataactt gcagagttta ttaagtatca ttgcagcaaa gatgaaatca
     2701 atgatttatc aaaaatgatt gaaaggtggt tgtaaataat gttacaatgt gtgagaagca
     2761 gtctaaattc ttcgtgaaat agtgattttt gaagctaata aaaaacacac gtggaattta
     2821 ggaaaaactt atatctgctg ctaaatttaa ccgtttgtca acacggtgca aatcaaacac
     2881 actgattgcg tctgacgggc ccggacacct ttttgctttt aattacggaa ctgatttcat
     2941 gatgaaaaaa tcgttatgct gcgctctgct gctgacagcc tctttctcca catttgctgc
     3001 cgcaaaaaca gaacaacaga ttgccgatat cgttaatcgc accatcaccc cgttgatgca
     3061 ggagcaggct attccgggta tggccgttgc cgttatctac cagggaaaac cctattattt
     3121 cacctggggt aaagccgata tcgccaataa ccacccagtc acgcagcaaa cgctgtttga
     3181 gctaggatcg gttagtaaga cgtttaacgg cgtgttgggc ggcgatgcta tcgcccgcgg
     3241 cgaaattaag ctcagcgatc cggtcacgaa atactggcca gaactgacag gcaaacagtg
     3301 gcagggtatc cgcctgctgc acttagccac ctatacggca ggcggcctac cgctgcagat
     3361 ccccgatgac gttagggata aagccgcatt actgcatttt tatcaaaact ggcagccgca
     3421 atggactccg ggcgctaagc gactttacgc taactccagc attggtctgt ttggcgcgct
     3481 ggcggtgaaa ccctcaggaa tgagttacga agaggcaatg accagacgcg tcctgcaacc
     3541 attaaaactg gcgcatacct ggattacggt tccgcagaac gaacaaaaag attatgcctg
     3601 gggctatcgc gaagggaagc ccgtacacgt ttctccggga caacttgacg ccgaagccta
     3661 tggcgtgaaa tccagcgtta ttgatatggc ccgctgggtt caggccaaca tggatgccag
     3721 ccacgttcag gagaaaacgc tccagcaggg cattgcgctt gcgcagtctc gctactggcg
     3781 tattggcgat atgtaccagg gattaggctg ggagatgctg aactggccgc tgaaagctga
     3841 ttcgatcatc aacggcagcg acagcaaagt ggcattggca gcgcttcccg ccgttgaggt
     3901 aaacccgccc gcccccgcag tgaaagcctc atgggtgcat aaaacgggct ccactggtgg
     3961 atttggcagc tacgtagcct tcgttccaga aaaaaacctt ggcatcgtga tgctggcaaa
     4021 caaaagctat cctaaccctg tccgtgtcga ggcggcctgg cgcattcttg aaaagctgca
     4081 ataactgacg atgaggccca ggatattggg cctcctttct ttctcttttt ttcctgttgt
     4141 catctacact taacaaaaat acagcaagga aaatcccatg cgcattttgc ccgtcgttgc
     4201 tgcagttacg gctgcattcc tggttgtcgc gtgtagctcc ccgacaccgc cgaaaggcgt
     4261 taccgtggta aataactttg atgccaaacg ctatctggga acctggtatg aaattgcgcg
     4321 cttcgaccat cgtttcgagc gcggattgga taaagtgacc gcaacataca gcttgcgcga
     4381 cgacggcggc atcaacgtta ttaacaaggg ctataaccct gacagggaga tgtggcagaa
     4441 aacggaaggg aaagcctatt tcaccggcga cccaagcaga gccgcgctta aggtttcttt
     4501 tttcggcccc ttctatggcg ggtataacgt aattgcactc gaccgggaat atcgtcacgc
     4561 gctggtttgt ggtccggatc gcgactacct gtggatcctt tcacggaccc ctactatttc
     4621 agatgaaatg aaacagcaaa tgttagccat cgcgacccgg gaagggtttg aagtgaataa
     4681 actgatttgg gtgaaacagc ctggcgctta gtgagtgctc agcttcagac caataatgcc
     4741 agcaacgatc agcccaaggc tcagcaaacg tgccgggctg gcagactcac ccagcagcaa
     4801 aatccctgta atggccgccc caacagcgcc aataccggtc cagaccgcat aagcggttcc
     4861 tacaggcaac gtgcgcattg cccaagagag catggcgata ctgacgatca tcgccgcaat
     4921 agtgataatg cttggcgtaa gacgcgtaaa accgtgggtg tatttcaggc caatcgccca
     4981 gacaacttcg agcaaacctg caattaataa aacgatccag gacatatcag gctccagaac
     5041 aatggggccg tccccggtga aagaagcgtt tgcaggtggg ttattcttgc tttttgccgg
     5101 agtgccgatg gcagaatcat ctgcagtgaa gggtatgctc atgctcttta tcaactgaca
     5161 tgtccggtgc ctgtggacag caaactggaa cgaaacacgc tcactgctct tctgaatgtg
     5221 gccagctggc ttaaaagaaa gccaggtacg ccggaattaa gtctggaaag gcccctgttt
     5281 gatacagaag tttatgttaa tggtgaaaag aaatatgtac tgccggattt cattgtcaca
     5341 gcaagggctc ctgacggaaa gacggccaga gtggtcatcg aaacgatggg atatgaagac
     5401 agtgattact gcgcgagaaa atccaggcag cataccggca tgaagcagat tggtgttctg
     5461 cataccgatc caccgaaatg gctggataac gatcatcccc cttttaagaa acatatgtac
     5521 ggtgttttta tgcatctcag gtactgagat attttgtggc tcagttctgt aacttttccc
     5581 gtaacattgt ctgttgttac gggaaagtcc ggtttttgta ttgcaccaga gaatacccag
     5641 actgtgatgc tgccacagtg tcagcaggct ttctgaacgg tataacacca cttcatgtta
     5701 atgataatta ctatcattaa atcttgacat gcca
//
LOCUS       DAPGWD010000218         5722 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPGWD010000218 DAPGWD010000000
VERSION     DAPGWD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.220"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(337..1296)
                     /gene="nrdF"
                     /locus_tag="RHX88_004152"
     CDS             complement(337..1296)
                     /gene="nrdF"
                     /locus_tag="RHX88_004152"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDV2232764.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLDYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1306..3450)
                     /gene="nrdE"
                     /locus_tag="RHX88_004153"
     CDS             complement(1306..3450)
                     /gene="nrdE"
                     /locus_tag="RHX88_004153"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDV2232765.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASSEYFSQYLQGNWQPKTTKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3423..3833)
                     /gene="nrdI"
                     /locus_tag="RHX88_004154"
     CDS             complement(3423..3833)
                     /gene="nrdI"
                     /locus_tag="RHX88_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDV2232766.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3830..4075)
                     /gene="nrdH"
                     /locus_tag="RHX88_004155"
     CDS             complement(3830..4075)
                     /gene="nrdH"
                     /locus_tag="RHX88_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDV2232767.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4323..4652)
                     /locus_tag="RHX88_004156"
     CDS             complement(4323..4652)
                     /locus_tag="RHX88_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV2232768.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4804..5148
                     /locus_tag="RHX88_004157"
     CDS             4804..5148
                     /locus_tag="RHX88_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDV2232769.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5185..5634)
                     /gene="alaE"
                     /locus_tag="RHX88_004158"
     CDS             complement(5185..5634)
                     /gene="alaE"
                     /locus_tag="RHX88_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDV2232770.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1469 a         1407 c         1417 g         1429 t
ORIGIN      
        1 tatttgttta tgtcgatata ttacccaatg gaaatagtca cttttttcta ccctaacata
       61 ctgagaatct gaggcaaccc ctgagggcaa atgtggcatg aatattgatg cgagcaaatc
      121 gccggaattt cccgtgatat aagggctgag agcaaatcga gaaaagttga tattttttgt
      181 catagatagc gaaaaaacgc ctgaatattg tagtttcagg cgaataatgc aacagagtat
      241 cagtgtagat cactacagaa tatttgcgtg ataaaaagct atttggcggg aattatttcc
      301 ctgctgcggg tagtgatatt tttgaaaata acaccctcag aaattccagt cttcatcttc
      361 tgtttcaacc gctttcccca tcacataaga ggagcctgaa ccggaaaaga aatcgtgatt
      421 ttcatcggca ttcggcgaaa gcgcggcgag gattgccgga ttcacttccg ccatttctgc
      481 gggaaataac ggttcgtagt ccagattcat caaagcctta ttggcgttgt aacagagaaa
      541 cgctttcaca tcgtcagccc acggggtttc ggcgtacagc tcatcggtgt attgcaactc
      601 gttgtcgtag agttccagca acaaatcgaa ggcgaaactc ttcaactctt cacgttgtcc
      661 cagagatatc ttttccatgt ttttctgata tttatagcct atgtagtaac cgtggactgc
      721 ttcatcgcgg ataatcagac ggatcagatc tgctgtattg gtcagctttc cgcggctgga
      781 aaaatacatc ggcagccaga aaccggaata gaacaaaaaa gattcaagaa acacactggc
      841 gattttcttt ttcagcggat catcaccgcg ataatgttgc tgaataatct gagcttttcg
      901 ctgcaacggt gcgttttctt cactccatgc gtaggcggca tcgacatctt tggtctgaca
      961 cagcgtcgag aaaatcgaac tgtaagagcg ggcatgaacc gcttccataa agctgatatt
     1021 cgataatacc gcttcttcat gaggcgtgag tgcatcgggc atcagcgaag gcgcgccgat
     1081 aacattttgc agcgtgtcga gcagcgtcag gccagtaaaa acgcgcatcg tcagttgttg
     1141 ttctacgaca gttaatgtct gccaggcagg aatatcgttc gacagcggca ccttttctgg
     1201 tagccagaaa ttgctggtca ggcgattcca cacctccaga tctttatcgt cagatatctt
     1261 gttccagttg atggcgctga tacgtgagag tttcatagat attccttaaa gtgcacagga
     1321 gacgcagcct tcaatttcag tgccttccag cgccatctga cgcaggcgaa tgtaatagag
     1381 cgttttgata cctttgcgcc aggcgtaaat ctgcgctttg ttgatatcgc gagtggtggc
     1441 ggtatcgggg aaaaacagcg tcagcgacaa accctgatcg acatggcgag tcgcttccgc
     1501 gtaggtgtcg atgatctttt ctgcgccgat ttcgtaagca tcctgataca gcgccagatt
     1561 ctcgttagtc ataaacgggg cagggtagta aacgcgtcct gttttgccct ctttgcgtat
     1621 ctctactttc gccacaatcg gatgaatact cgacgtagca tggttgatat aagagataga
     1681 accggttggc ggcaccgcct gaagattctg gttataaatg ccgtagcgca tcacgtcgtc
     1741 gcgcagttgc gcccacatct cacgggtagg taacgtaata ccgctacggg caaacagttc
     1801 gccaactttc gtcgttttcg gctgccagtt cccttgcaga tattggctga aatattcact
     1861 actggcatag cgtgactgtt tgaacccggc gaaggtttca ccgcgttcgc gtgccagcaa
     1921 catcgaggta cgcagtgcgt gccaggtgat ggtatagaaa tagagattgg tgaaatccag
     1981 tgcttccggc gaaccataag cgatgccttc tcgcgccaga taaccgtgta aattcatctg
     2041 ccccagcccg atggcgtgtg aggcggcatt tccggcttcg atggacggca cgctgcggat
     2101 atgactcata tctgacaccg ccgttaaacc gcgtacggca gtctctaccg tgcgggcaaa
     2161 gttgggggaa tccatggtgt gggcaatatt caacgaacct aaattgcagg aaatatcatg
     2221 gcctgtacgg gcatagtcga gattctcgtc atactctgag gcgctgttaa cctgcaaaat
     2281 ttctgagcat agattactca tatttatgcg cccggcgata gggttagcac ggtttaccgt
     2341 gtcttcatac atgatgtagg gatagccgga ctcaaactgg atttctgcca gtcgctggaa
     2401 gaaatcacgg gcgttgaggt attttttgcg gatgcgttca tcggcaacca gttcgtcata
     2461 gtgttcgcta atggcgatat cggcaaacgg cttgccataa actcgctcaa cgtcataggg
     2521 cgaaaacagc gccatctgcg cattctcttt ggccagatgg aaagtgatat ccgggatcac
     2581 cacgccaagc gacagtgttt taatgcggat tttttcgtcg gcattttccc gtttcgtgtc
     2641 gagaaaacgc agaatatcgg gatgatgagc atgtaaatag actgcaccag ccccctgacg
     2701 agcgccgagt tggttggcat aggaaaatgc gtcttccagc attttcatca ccggaattac
     2761 gccagaagat tgattttcaa tacgtttaat tggcgcgccc gcttcccgca gattcgacag
     2821 caaaaatgct acgccgccgc cgcgtttcga cagttgcagt gcggaattta ccgcccggcc
     2881 aatcgactcc atattgtctt caatacgcag caaaaaacag gaaaccagtt cgccgcgctg
     2941 ctgcttacca cagttgagga atgtcggtgt tgcaggctga aagcgtcctg acagcatctc
     3001 atcggtcagt tgcaacgcca gtgtttcatc gccttgtgcc agcgttagcg ccaccatcgt
     3061 gactcgatcg gcaaaatctt ccagataacg cttaccgtcg aatgtcttca acgtatagct
     3121 ggtgtaaaac ttccatgccc cgaggaatgt atggaaacga aaaccgctgg tgtgtgcgtg
     3181 ggcaaacagc gtaatgacaa agtcgcgaga gtagcgatta agaacgcttt catcatagta
     3241 accttcgttg accagccagt tcaggcgctg ctgctggcta ctgaaggtca cactgttcgg
     3301 acgcacatgc gtcgccataa aggcgtcaac ggcctggcgg tctttatcga actgaatgcg
     3361 acctgcgcta tcgtagaggt taagcatcgc attcagcgcg tggtaatcca tcgtttcctg
     3421 cgtcaggcat tctgcggttg tcgttgccaa aattcggtta ctcctttacg aacgttttcg
     3481 atatcgcttt gcgtacccat gagttcaaaa cggtacagcc acggcacgcc gcatttctga
     3541 gcaatcacat ctccggcacg gccatacgcc tcaccaaagt tgcgattacc cgaagcaata
     3601 acgccgcgaa gcaacgcccg gttgtgctcg tcgtttaaaa agcgaattac ctgtcgtggc
     3661 accgcgccag ccgtaccgcc gccgccgtaa gagggcacga tcaggatgta aggctcgtct
     3721 acctgaatcc gttcccgctc attgagcggg atgcgcaccg cgggcagacc taaacgttcg
     3781 ataaaacgct gcgtattttc ggagctgctg gagaagtaga cgagctggct catgcactgg
     3841 ccgcgtgtgg cgctggatgc agacggttaa tcatgtccgg acggaaacca gaccagctaa
     3901 gatcgccagc aatcactacc ggcaactgac gaaagccctg agcacgcaac gcttccgctg
     3961 cttcaggaac gcgatcgaca ttaatcattt caaaatcaaa gccccggttt tccatcgccc
     4021 gtttggtggc gtggcactga acgcaatcgt tacgagtgta aatagtaatg cgcatgattc
     4081 gtatttccgt ttaaaatgaa gatacggcgc gatgatacgc gtcgggttgt ctctctgttg
     4141 atacagagat actagatgta gttgaaaaaa gattcaacca cacaatatat agcaaatagt
     4201 ggtcgaaatt accctggata tgagcgtgac ggggtagggg gatttttgtg attcaccagg
     4261 caaaaagaaa ccccgaagac aggcttcggg gtcaaagacg cgtatttatt atcatttttg
     4321 cactacgatt tgcgcatgct taacagtgcg ccgataaaaa tacctaccgc agctgctgta
     4381 cccacgctac accagggtct ttcacgaaca aaagaatccg cgcagccaac ggcatcgcgc
     4441 gcggcttgct ggacgcgagt acgaccatgc attcgcgccc tggtttcttt cagcaacgcc
     4501 tgagctttac tgcgtgcggc ttcagcttcc cctttggcgt cgctgcccca ggacttcaat
     4561 acagattcga ggctgtcagc taattgattg acatcattct ggatatcctg cacgccatca
     4621 tcaacgtcgt tgcggttcgg tcggttaaac atatgatccc ccattagaat tcgatgtaat
     4681 tttcagtgta gaccataatt ttcttaactg aggttcatcg cggctttatg gacttttctg
     4741 aaagcgttgt gaatgcgcaa tcaggtaagg taggcccgcg gttgataata acgaggatca
     4801 aatatgtatt tacgaccaga cgaggtggcg cgcgtacttg aaaaagtcgg ttttactgtc
     4861 gatgtggtaa cccagaaagc gtatggttac cgccgtgggg aaaattatgt ctatgttaat
     4921 cgcgaagcgc gaatgggacg taccgctctg gtaattcatc caacattaaa agaacgcagt
     4981 tcaacactgg ctgaacccgc ttccgatatt aaaacttgcg atcattacca gcaatttccg
     5041 ctctatttag caggcgagcg acacgagcat tacggtatcc cgcatggctt cagttcgcgt
     5101 gttgcgcttg aacgttattt gaatggttta tttggcgaag ccagttaaag acgcgactgg
     5161 cgatgccagt cgcgaaaaga agagtcaggc ttttacctgc tggtaacggc tgactttaaa
     5221 cagtcggcgg caataatcga ggaagtagcc ataaaccgcc cccatcaaca tcgaaacaac
     5281 gatgtttgaa ctgaccgccg ccataatctg atgccagtct gcgcccacca ctaacaagat
     5341 cgccacatac accggtgact ggaacgtcac ataagccagg atatcagcca gattttttat
     5401 ccagcccgac gggctaactt tgcgtgccgc gcgcataaac agatcgcggt acataccgta
     5461 tggccatgca attaagatgt tcaccggaat cgctaccaat ctggaataaa aagactgttc
     5521 gaagctcatt ccggagagga aaacttcaat acacatgttc acgacagaac agtaaacaac
     5581 catcgcgaac gtatctgcaa ctgcatgacg caagcgtgac tgcggtgaga acatgctaat
     5641 gctccttaat aaaagcgtaa tgggatgtta atggagatga aagcagtgga tctcattgtc
     5701 atctgcattt tcgcatagcg ta
//
LOCUS       DAPGWD010000219         5703 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPGWD010000219 DAPGWD010000000
VERSION     DAPGWD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.221"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            115..765
                     /gene="csgD"
                     /locus_tag="RHX88_004159"
     CDS             115..765
                     /gene="csgD"
                     /locus_tag="RHX88_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDV2232771.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            770..1159
                     /gene="csgE"
                     /locus_tag="RHX88_004160"
     CDS             770..1159
                     /gene="csgE"
                     /locus_tag="RHX88_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDV2232772.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1184..1600
                     /gene="csgF"
                     /locus_tag="RHX88_004161"
     CDS             1184..1600
                     /gene="csgF"
                     /locus_tag="RHX88_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDV2232773.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1627..2460
                     /gene="csgG"
                     /locus_tag="RHX88_004162"
     CDS             1627..2460
                     /gene="csgG"
                     /locus_tag="RHX88_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDV2232774.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2524..3015)
                     /locus_tag="RHX88_004163"
     CDS             complement(2524..3015)
                     /locus_tag="RHX88_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV2232775.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3117..3671)
                     /gene="ycdY"
                     /locus_tag="RHX88_004164"
     CDS             complement(3117..3671)
                     /gene="ycdY"
                     /locus_tag="RHX88_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDV2232776.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDKCAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3695..4432)
                     /gene="ycdX"
                     /locus_tag="RHX88_004165"
     CDS             complement(3695..4432)
                     /gene="ycdX"
                     /locus_tag="RHX88_004165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDV2232777.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4487..5425)
                     /gene="ghrA"
                     /locus_tag="RHX88_004166"
     CDS             complement(4487..5425)
                     /gene="ghrA"
                     /locus_tag="RHX88_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDV2232778.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT         1546 a         1407 c         1322 g         1428 t
ORIGIN      
        1 ttagggctga tttattacta cacacagcag tgcaacatct gtcagtactt ctggtgcttc
       61 tattttagag gcagctgtca ggtgtgcgat caataaaaaa agcggggttt catcatgttt
      121 aatgaagtcc atagtattca tggtcataca ttattgttga tcactaaacc gtctttgcaa
      181 gcgacagctc tcttgcagca ccttaaacaa tcgctggcaa ttacaggaaa attacataat
      241 attcaacgtt ctctggacga tatatcttca ggctctatta ttcttctgga tatgatggaa
      301 gcagataaaa agcttatcca ttattggcag gatactttga gcaggaaaaa caacaatatc
      361 aaaatattgt tgctaaatac gcctgaagat tacccgtacc gcgacattga aaactggcct
      421 catatcaacg gcgtttttta tgccatggag gatcaagaac gtgttgtcaa tgggttgcaa
      481 ggcgtcctgc gcggcgaatg ctactttacg caaaagcttg ccagttacct gattacacat
      541 tctggtaact atcgttataa cagcacggaa tcagccctcc ttactcatcg ggaaaaagag
      601 atcctgaata agctgcgtat cggcgcgtct aataacgaga tcgctcgttc gttgttcatc
      661 agcgaaaata cggttaaaac gcatctttat aatcttttca agaagatagc cgtaaaaaac
      721 cggacacaag cggtttcctg ggcaaacgat aacctcaggc gataaagcca tgaaacgtta
      781 tttacgctgg attgtggcgg cagaatttct gttcgccgca gggaatcttc acgccgttga
      841 ggtagaagtc ccgggattgt taactgacca tactgtttca tctattggcc atgattttta
      901 ccgagccttt agtgataaat gggaaagtga ctatacgggt aacttaacga ttaatgaaag
      961 gcccagtgca cgatggggaa gctggatcac tataacggtc aatcaggacg ttattttcca
     1021 gactttttta tttccgttga aaagagactt cgagaaaact gtcgtctttg cactgattca
     1081 aactgaagaa gcactaaatc gtcgccagat aaatcaggcg ttattaagta cgggcgattt
     1141 ggcgcatgat gaattctaaa taaaaaattg ttcggaggct gcaatgcgtg tcaaacatgc
     1201 agtagttcta ctcatgctta tttcgccatt aagttgggct ggaaccatga ctttccagtt
     1261 ccgtaatcca aactttggtg gtaacccaaa taatggcgct tttttattaa atagcgctca
     1321 ggcccaaaac tcttataaag atccgagcta taacgatgac tttggtattg aaacaccctc
     1381 agcgttagat aactttactc aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa
     1441 tattaatacc ggtaaaccgg gccgcatggt gaccaacgat tatattgttg atattgctaa
     1501 ccgcgatggt caattgcagt tgaacgtgac agatcgtaaa accggacaaa cctcgaccat
     1561 ccaggtttcg ggtttacaaa ataactcaac cgatttttaa gccccagctt cataaggaaa
     1621 ataatcatgc agcgcttatt tcttttggtt gccgtcatgt tactgagcgg atgcttaacc
     1681 gccccgccta aagaagccgc cagaccgaca ttaatgcctc gagctcagag ctacaaagat
     1741 ttgacccatc tgccagcgcc gacgggtaag atctttgttt cggtatacaa cattcaggac
     1801 gaaaccgggc aatttaaacc ctacccggca agtaacttct ccactgctgt tccgcaaagc
     1861 gccacggcaa tgctggtcac ggcattgaaa gattctcgct ggtttatacc gctggagcgc
     1921 cagggcttac aaaacctgct taacgagcgc aagattattc gtgcggcaca agaaaacggc
     1981 acggttgcca ttaataaccg aatcccgctg caatctttaa cggcggcaaa tattatggtt
     2041 gaaggttcga ttatcggtta tgaaagcaac gttaaatcgg gcggtgttgg ggcaagatat
     2101 tttgggatcg gtgcagacac gcaataccag ctcgatcaga ttgccgtgaa cctgcgcgtc
     2161 gtcaatgtga gtaccggtga gatcctctcc tcggtgaaca ccagtaagac gatactttcc
     2221 tatgaagttc aggccggggt tttccgcttt attgactacc agcgcttgct ggaaggggaa
     2281 gtgggttaca cctcaaacga acccgttatg ctgtgtctga tgtcggctat cgaaacaggg
     2341 gtcattttcc tgattaatga tggtatcgac cgtggtctgt gggatttgca gaacaaagca
     2401 gaacggcaga acgacattct ggtgaaatac cgccatatgt cggttccacc ggaatcctga
     2461 cagataaaaa agccggaatt atcatccggc attttactgt ttaagcgaaa gcgtgagtgc
     2521 ccctcacgct ttatttttga tttcctgctc acggtgagcc gtctttgcac tacgcgccgc
     2581 cagccacagg ccgctgtttt tcattgcgta accaaatacc agcccaagcg ccagcgaagg
     2641 taacaccagt ttccagtcac cctgcccggc aaacgtcgcg catgcgccta taaacgtccc
     2701 cggaacaaat gaaagcagca gctgtttggc ctgaatacac atcagaaacg cgacaatacc
     2761 ggttatgaca taaccgagaa tttccagatg tggtgccagt gcactaccgt aaataatgac
     2821 catcgcccac accacgccac taagcagggt tgcagcggag atcgccagcc ctttcagccc
     2881 accttgcggg caggcaaagt aggccgtaca gcccaggaag cccgcccagc ttagtaagcc
     2941 cagggaaaca gccacccatc cccagatacc ggagagaatg cctgttgtga ttgcaataga
     3001 gagaagtatg ttcatgccgc gcatgatagc agaaaacgcg gccatgaatg agatcgagca
     3061 cacattttat gcaacaacaa attatctgtt gcagttatga gtgacgatga tcacatttac
     3121 tcttcagaat cttcttccag ctcgtcccac attgcactaa tggcatcacg ggttagcggt
     3181 gccatggtgc gccagaaagg cgtggttgca tgggcctcca ctttgccgag gaacgcacca
     3241 caccagggta acagatactc actgaacagt gtttccagtg cttcgctctc atcttccgtt
     3301 gactgatctt ccagccagga agccgcaagc agcaatgtgc cgatgtgatc ggctggcgta
     3361 tccgccagtg gcatccctcg ttcggaaaga aaagcgcgca cttccgcttc cgttgcgtcc
     3421 tcaacccatg cgctacgata tggcggcacg gcacatttat cgccgataaa caacgcattg
     3481 taatcggcag agacttgcgc catatcacag cttttctgta aacgcgtcag taactcatcc
     3541 tgctccagtg gccagttcgc agccagtttc ccctcacgaa tcagggtaaa cagcggcacc
     3601 agtaaaggat cttgcggttg gcggtaatag agcgaaccca gcacacgaca gaggatagaa
     3661 aactcgttca ttaatatatt tccattacta gtaattaaag gtctgcaaat tccgcaatcg
     3721 gtgccatacc gcgagattca aggaagttca gtaagcggcg cggcgaaaca ttcaaaatgc
     3781 gctctggcgg aaaatctacc gcgtcgagga ttttaagaca ctcttcaaat tcccccatgg
     3841 taaacgcggt gtgagaatcc gagcctaatg ccacccaacc tccagcatca cgtaccgctg
     3901 cggctaccgc acgacagttg tcttcactgc ccttacgtga gtgtaaaaat gaagaattat
     3961 tgatttccag cgccacctga tgtttcgcgg ctgcttcagc aacggctttc acatctattt
     4021 catatttggg atttccggga tggcttatta tatgcacatt gccgctggcg atagtggcga
     4081 tcatcgcttg tgtattggtc gctttgtcat gtggtgcaaa aaccggctca tgaaaaccgg
     4141 caataatcag atccagcgag tcaaacattt taccgctgca atcaatttca ccatcaacat
     4201 ttttaatgtt agcttcgatg ccgcgcagga tccctacccc atcaaccact cgcggccaga
     4261 tacgcatgtt aatgaagtgc cagtgatgcg gagcatcttc catatccggg ccatgatcgg
     4321 tgatcgcaaa aagtttaatg cccttttgtt tggcctgggc aatgtaatca ctcaatgtgc
     4381 tatatgcatg tgtgctggca acggtatgca tatgaaggtc gacgggatac ataactctct
     4441 cctgtattca attcccgcca aggatagcag gaatcctgat gctttattag tagccgcgtg
     4501 cgcggtcgac ttgcccgcag accctctccc ctttttcgag ctgggcaatg gtgcgagaaa
     4561 tgtactccac agcttcagcg ggacgggtaa tcgcggcgac atgtggtgtt atcgtcacgc
     4621 gtggatgttg ccagagcgga ctttcaggcg gtaagggttc acgattaaaa acatccaaca
     4681 ttgcgccttt aactttgccg ctatccagcg ccgcgagcag gtcatcttcc acaacatgaa
     4741 caccacgcgc caggttgagg agatacgcgc catccggtaa tttttcgagt aattgttgat
     4801 taataatgcc gacggtttca ggggtattcg gtaacaaatt aatcaatacc cgacattggc
     4861 tcagaaatgc agacagttct tcccgtccgg caaagctttg cacgccaggc cacgatttac
     4921 gggttcgact ccagcaacgc agcggaaagc gccaggtttg cagactctga gcaactttac
     4981 tgcccagtac gcctgcgccc aaaatgccga tggtaaaatc ttcccgatga tattcaggca
     5041 gcggttgcca atgcgaacta ttttgctgga tgcgataatc gtcaaaacgt cgaaaccaat
     5101 gcagcacctg actgacagca tattcctgca tttgctcgcc cataccggta tcttccaggc
     5161 gaaaaagtgg aacagaaggg ttcagcattt cagggtgtgc ctgtagcttg ctcaaaatag
     5221 aatcaacacc ggccccgagt gcgaacaccg ctttaagatc gcgccctgcc agcatttcaa
     5281 caggaggatg ccagactaaa gcataatcag cagagtcatt atctccgctt ttccacgctc
     5341 tgactcttgc ctgaggaata gctttgcgca gtgcctcaat ccaccattgg gtatcgaacg
     5401 ttgggtgata aaagatgata tccatactga ctcccgaaaa gcgttgtgcg aaatttattc
     5461 gcacttatcg ttatgatcta caaaggccac cagcataaca aatccgtggt cggtggcaaa
     5521 aaaagcagat ttcgcttatt aaaaccacac attgattgaa gtttgaataa acgcgcgatt
     5581 ttttcaaaaa gtttgttgac ctcaggtcat gatttcccta aattagcgcc cgttccagca
     5641 agacaggaac gacaatttgg tgaggtgtcc gagtggctga aggagcacgc ctggaaagtg
     5701 tgt
//
LOCUS       DAPGWD010000220         5671 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPGWD010000220 DAPGWD010000000
VERSION     DAPGWD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.222"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(55..441)
                     /gene="ridA"
                     /locus_tag="RHX88_004167"
     CDS             complement(55..441)
                     /gene="ridA"
                     /locus_tag="RHX88_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDV2232779.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(514..975)
                     /gene="pyrI"
                     /locus_tag="RHX88_004168"
     CDS             complement(514..975)
                     /gene="pyrI"
                     /locus_tag="RHX88_004168"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDV2232780.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(988..1923)
                     /gene="pyrB"
                     /locus_tag="RHX88_004169"
     CDS             complement(988..1923)
                     /gene="pyrB"
                     /locus_tag="RHX88_004169"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDV2232781.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(1927..2061)
                     /gene="pyrL"
                     /locus_tag="RHX88_004170"
     CDS             complement(1927..2061)
                     /gene="pyrL"
                     /locus_tag="RHX88_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDV2232782.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(2342..2737)
                     /locus_tag="RHX88_004171"
     CDS             complement(2342..2737)
                     /locus_tag="RHX88_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV2232783.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRQAFNNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(2868..3581)
                     /gene="bdcA"
                     /locus_tag="RHX88_004172"
     CDS             complement(2868..3581)
                     /gene="bdcA"
                     /locus_tag="RHX88_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2232784.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3652..4245
                     /locus_tag="RHX88_004173"
     CDS             3652..4245
                     /locus_tag="RHX88_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV2232785.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAKCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILEE"
     gene            4390..4842
                     /gene="tabA"
                     /locus_tag="RHX88_004174"
     CDS             4390..4842
                     /gene="tabA"
                     /locus_tag="RHX88_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDV2232786.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTPETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPETDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            4965..>5671
                     /locus_tag="RHX88_004175"
     CDS             4965..>5671
                     /locus_tag="RHX88_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV2232787.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILFPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMQF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLL"
BASE COUNT         1462 a         1304 c         1408 g         1497 t
ORIGIN      
        1 ttgcgtttgt catcagattt aatccgggca tgatagcccg gatttccatc aagattagcg
       61 acgaacagcg atcgcttcga tctcaatttt tacgtctttc ggcagacggg caacttcaac
      121 gcaagaacgt gccgggaagg tggcgttgtg ttcggtgaag aaggcttcgt aggtagcatt
      181 aacggttgcg aagtcgttca gatctttcac aaacacggtg gttttaacga tgtcgcccac
      241 tttcaggcca gcggcttcga cgatcgcttt tacgttatcc agcgactgac gtgcctgtgc
      301 agcgacgtct gccggtactt cacccgtttt cggatttacc gggatctgac cggaggtgat
      361 gatcatattg cccagatcaa cgccctgtac gtaagggccg atagctgccg gtgcattttc
      421 cgtcgcgata gttttgctca tgatttctcc tttattacag cggtaaaaag tctggctcat
      481 tatagggagc cagactttta ttaccaaccg caattaattg gccagcacca cattatggga
      541 aaactctttt tcacagtatt tgcatttgag cgcgatgtca ttggcgcgtt ttcgcacggc
      601 aaagctggaa gaaaccggtt cggcatggct gatacagttg ctgttcgggc agaccagcac
      661 attgtcgatg cgctccggca gactggggcg cgatttaccc accacttcat agttgtcgat
      721 acggttaacc gtggcttgcg gcgcatacaa tgccagttga tctacctgat cttcactcaa
      781 aaaggtattt tcgattttga tcagatcttt gcggcccatc tcgccagaag gcaagttcag
      841 accgatggtg atgcgctgat ccgtttcggt cagcttgaac agactcaaca gcttaaaacc
      901 gatctgggcg gggatatggt caattaccgt gccgcgttta atagcttcaa cctgcaattt
      961 attatcgtgt gtcatctcta tttcccctta cagtaccaga tcgcgattca gaaccagtgc
     1021 cagtaacgcc tggcgagcga atatcccatt gcctgcctgc tggaagtacc aggcgtgtgg
     1081 cgttttatcc acatcggtgg caatctcatc aacacgcggc agcggatgca gcactttcat
     1141 attggctttg gcgttgtgga gatcgctggc gcgaagaaca aactgcgctt ttacgttggc
     1201 gtattcggat gggtccaggc gctctttttg cacgcgggtc atatacagga tgtctacttc
     1261 cgccatcact tcttcaatag agctgtgcag actccaggcg atcccttttt catcaagcat
     1321 atccagaatg tattgcggca ttgccagcgc gtccggcgcg atgaagtaaa aacggttgcc
     1381 gtcgaacttc gctaacgcct gagtcaggga gtgaacggtg cggccatatt tcaggtcacc
     1441 aaccattgcg acgtggagat tgtccagacg cccctgggtt tcctgaatgg tgaataagtc
     1501 cagcaaggtt tgcgtcggat gttggttgga gccatcaccg gcattcagta ccggtacatt
     1561 gccggaaaac tcggtggcca ggcgcgccgc accttcctgc ggatgacgca tcactatcgc
     1621 atcgacgtaa gtgctgataa ccgaaatggt atcggccagc gtttcgccct ttttacccag
     1681 tgatgtattg gcgctgtcgg aaaagcccac cacgctggca cccaggcggt gcatggaagt
     1741 ttcgaaagag aggcgggtac gggtagaggc ttcgaagaaa cagctggcaa tgactttgtg
     1801 cttcaacagc tctggttgcg ggtttgcttt cagtttcgcc gctgtcgcca gcaccagatt
     1861 aaggtcatcg cgactaaggt cgtttatgga aatgatatgt ttctgatata gcggattagc
     1921 catcttttat ctcctgacgc ctgggcaaaa aaaagcccct cgattgaggg gctgggaatg
     1981 ggtgatcaac gggaagaaaa acggcaggcc agcgtctttt ttcagacgcg gtaagacaaa
     2041 atgtcgaaca cactgaacca tacatcctcc cggcaaattg tccggcatta tactcatcgt
     2101 cagaagcggc gcaagcattt gatgcaatat tttgtcagcg caaacggttt atttgaatta
     2161 aaagtcaagg tatatgcatt tttattcgat gtgattctgg aggggaactg tccttcggta
     2221 tcaataattg cagacatttc ctgctttttt ctttttcact ttcacgcaat cagatatgca
     2281 ttttattcca ttcatccgac ttatagggcg gagtgtgaaa acgaacggct aacactattg
     2341 cttactgctc agggatgcgc gctatcactt taatttcaaa atcaaagcct gccagccatg
     2401 taacacctac cgccgtccag tttggataag gtggggcgct aaatatttca tttttcaccg
     2461 tcatgatgtc ttcaaattgg ttttctggat cggtatggaa gctcgtaaca tcaatgatat
     2521 cgtcaaaagt gcatcccgca gcagccaggg tcgcatgcaa attattaaat gcctgtctga
     2581 cttgttgctg aaaatcgggt tctggtgttc catcctctcg acttcctact tgcccggaaa
     2641 caaacagcaa atcgccggaa cgaatagccg cagaataacg atgctcagca tacagtgaat
     2701 gtcggccagc agggaaaaca gcggttcttt ctaccatttg gttatcctca agatttacga
     2761 catgaacaga agagttctct ttaccgggag ccgcttttag cggacgacgt gagtaaacaa
     2821 aacccagaca tcatggataa tggctgggct taattgagcg tagtcggtta tgcgccaaac
     2881 gcgccatcaa tggtatgcat cgcgccggta acaaagctgg cttctggccc tgctaaccat
     2941 gcgaccatac cagcgacctc ttccggttgc ccatgtcttt tgatagccat aaaaccatgc
     3001 aacatatcgc gcattggccc gttggcggga ttagcgtcgg tatcaattgg ccctggctgg
     3061 acgacgttga tggtgatccc acgcggtcca aaatcacggg ccagcccgcg cgccatgcct
     3121 tgcagggcag atttactggc ggcataagca gccatgcctg caacaggcat acgatcgcca
     3181 ttcacggagc cgatgattaa gatgcgcccg ccttcgggca tctgccgggc ggcttcaacg
     3241 gaggcatggt aaggagcatg aatattgatt ttgaaaaggc gatcaatatc gtcggcattt
     3301 aattccaggg catcgccaaa gacgccaata cctgcattaa ccaccaggat atccaatgcg
     3361 ccgctcttac gaacgacatc aatgacagcg tctctgtcag cactatctgt gaatactgct
     3421 gtcgctccag tctcttgtgc caggcgttca gcggcatctt tcgaccccgc ataggtgaat
     3481 cgtacattgg ccccatcggt gacgaaacga cgtacgatag cggcaccgat tccacggctg
     3541 ccaccgagga tgagaactgt cttacctgta aaagcgccca taaggactcc ttgatttatt
     3601 atgtaacatg cattacaaaa ctgttttaac tttctgtcaa caagttttgt aatggtcact
     3661 aaaaaacaat ctcgcgttcc aggtcgtccc agacgtttcg ctcctgagca ggcagtctct
     3721 gcggcaaaag tgctttttca ccaaaaaggt ttcgatgctg tcagtgttgc tgaagttact
     3781 gattatcttg gtattaaccc cccgagtctc tacgcggctt ttggcagtaa ggctgggtta
     3841 tttagccgtg tactcaatga atatgtcggt acggaagcta ttccgcttgc cgatattctt
     3901 cgtgatgatc gtccggtagg cgagtgtctg gttgaggtat taaaagaagc ggcgcgcaga
     3961 tatagccaaa acggcggctg cgctggctgt atggttcttg aaggtattca tagtcatgat
     4021 ccacaagcgc gtgatattgc cgttcaatat tatcacgccg cagaaacgac catttatgac
     4081 tatatcgcca ggcggcatcc acaaagcgca aaatgtgtga ctgattttat gagtaccgtg
     4141 atgtcagggc tttctgcgaa ggcacgggag gggcactccc tcgaacaact ctgtgcaaca
     4201 gctgcactgg cgggggaagc gataaaaact attctcgagg agtgatgctg accttgaccc
     4261 gaatggcggg aacgcgcctg ccgataagtt gtgataagac aataattcac gcgttaaggc
     4321 tagcggaatt gatcatcttt tcgtataacg atagaaatga aacgttgttt taattaagga
     4381 gtggaaaaga tgatcatcgg aaatattcat aatcttcagc cgtggctacc ccaggagtta
     4441 cgccaggcga ttgagcatat caaagcgcac gttacgccag aaacgccaaa gggtaagcac
     4501 gatatcgaag gcaatcgtct gttttatctt atctcggaag atatgaccga gccgtacgaa
     4561 gctcgccgtg cggagtacca tgcccgctat ctcgacattc agattgtgtt aaaaggtcag
     4621 gaaggcatga ccttcagcac gcaacctgca ggcacgccgg agaccgactg gttagctgat
     4681 aaagacatcg catttttgcc ggaaggcgtt gatgagaaaa cagttatcct aaatgaaggt
     4741 gattttgttg tgttttatcc gggggaagtg cataaaccgc tgtgcgcagt gggtgcacca
     4801 gcccaggttc gcaaagcagt agtgaagatg ctgatggcgt gatgactttt cgccgtaaat
     4861 aactccaggt ttacggcgag tttgtgaaaa gagcgttttt tgatattttt ttgtgagtag
     4921 aatttgtaat gcttagacgt tcttattaac tcaaggagtc cgtcatgagc aaaatatcag
     4981 gttggaattt ttctcaaaac attacatcag ccgacaattg taaacaaaaa aatgaagact
     5041 tagatacctg gtatgtggga atgaatgatt ttgcccgaat tgccggaggg cagaatagca
     5101 gaagcaatat tctttttccc agagcatttt tggagttttt ggctaagata tttaccctgg
     5161 gttatgtgga ttttagcaaa cgctccaacg aagcgggtag aaatatgatg gctcatatta
     5221 agtcctcatc ttatatcaaa aataatgatg gtagtgagat aatgcagttt gttatgaata
     5281 atcctgaagg ggagcgagcg gatttatcta aggtggaaat agaaattaca cttgcctcag
     5341 cttttaataa tgggattcgt gaaggacata cagtcattat atttacacaa cctgatggtt
     5401 cgactaatcg ttatgaaggg aagtcctttg aaagaaaaga tgagagttca ttacacctga
     5461 ttactaacaa gattctggcg tgttaccaac gcgaagctaa caaggaaata gctcgtctat
     5521 taaatattcc gcaggagtta aataattctc aggacttgaa taactcgcag gtgagttgta
     5581 aagattcagt tgattctacg attacggatt tattagaaaa accattgaat aatgcattat
     5641 tagcaataag gaaagaacat ctgctattaa t
//
LOCUS       DAPGWD010000221         5577 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPGWD010000221 DAPGWD010000000
VERSION     DAPGWD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.223"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..698
                     /locus_tag="RHX88_004176"
     CDS             <1..698
                     /locus_tag="RHX88_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV2232788.1"
                     /translation="CMAFSSSSIADIVISGTRVIYKSDQKSVNVRLENKGNNPLLVQS
                     WLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVL
                     EVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPLALKWFWSGSEGKASL
                     RVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAI
                     NDFGGAIEGNASL"
     gene            733..3333
                     /gene="htrE"
                     /locus_tag="RHX88_004177"
     CDS             733..3333
                     /gene="htrE"
                     /locus_tag="RHX88_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HDV2232789.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3350..3919
                     /locus_tag="RHX88_004178"
     CDS             3350..3919
                     /locus_tag="RHX88_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2232790.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            3934..4536
                     /gene="yadL"
                     /locus_tag="RHX88_004179"
     CDS             3934..4536
                     /gene="yadL"
                     /locus_tag="RHX88_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HDV2232791.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4563..5159
                     /locus_tag="RHX88_004180"
     CDS             4563..5159
                     /locus_tag="RHX88_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2232792.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGGGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5212..>5577
                     /locus_tag="RHX88_004181"
     CDS             5212..>5577
                     /locus_tag="RHX88_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDV2232793.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMY"
BASE COUNT         1651 a         1142 c         1255 g         1529 t
ORIGIN      
        1 cctgtatggc ttttagttca tcgtctattg cggacattgt catttcgggt actcgcgtaa
       61 tatataaaag cgatcaaaaa agtgtcaacg tacgtctgga aaataaaggg aataacccgt
      121 tgcttgtcca gagttggtta gatactggcg atgacaacgc tgagcctggc agtattacag
      181 tcccttttac tgccacgccg ccagtatcgc gtattgatgc caaacgtggg caaacaatca
      241 aattaatgta cacagccagc acctcactgc ctaaagacag agagagcgtg ttctggttta
      301 acgtactgga agttccacca aaaccagatg cagaaaaggt cgcgaatcaa agtctgctgc
      361 aactggcatt tcgcacacgt ataaaacttt tctatcgccc ggatggattg aagggaaatc
      421 cctctgaagc cccgttagcc ctgaagtggt tctggtcagg ttcagaaggt aaggcgtcat
      481 tgcgagtgac caatccaacc ccttactacg tctcttttag cagtggtgat ttagaagcta
      541 gcggtaaacg ctatccgatt gatgtgaaaa tgattgcacc atttagtgat gaggtcatga
      601 aagtcactgg ccttaatggc aaagcaagtt ctgcaaaagt acatttttac gccattaatg
      661 attttggtgg cgccattgaa ggtaatgcca gtctgtaatc aggcaaggat ataattccgc
      721 aggaagcata acgtgactat aaaaccaact aaaaattatc ataatcatct gacccgtatc
      781 gctacgtttt gcgcgctgct gtattgcaat agtgctttct gtgctgaact tgttgaatat
      841 gaccatacct tcctgatggg gcagaatgcc tcaaatattg atctcagccg gtatagtgaa
      901 ggtaaccctg ctataccggg tatgtacgac gtcagtgttt atgtcaacga tcaacctatt
      961 attaaccaaa gcattacatt tattgaaatt gaaggaaaaa aaaacgcaca agcctgtatc
     1021 acacttaaga atttattgca gtttcatata aattctcccg atataaataa cgaaaaagcc
     1081 gttctgcttg ccagggatga aacactcggc aattgcctta atttgacgga aattatccct
     1141 caggcttctg ttcgttatga cgttaacgag caacgtctgg atatagacgt tcctcaggca
     1201 tgggtaatga aaaattacca aaactatgtt gatccatcgt tatgggaaaa tggcattaat
     1261 gcggccatgt tgtcatacaa cctcaacgga tatcatagtg aaactcctgg tcgaagaaat
     1321 gacagcattt atgctgcttt taacggtggg atgaatttag gtgcatggcg actgcgtgcc
     1381 tcaggcaact acaactggat gaccgattct ggcagtaatt atgattttaa gaatcggtat
     1441 atacagcgtg atatcgcctc gctgcgttct caactcattc ttggtgagtc ttatacaacg
     1501 ggcgaaacct ttgattctgt cagtatccgc ggcattcgtt tatacagcga cagccgcatg
     1561 ttgcctccga cattagcgag ttttgcgcct atcattcatg gcgttgctaa taccaacgcc
     1621 aaagtcacta ttacgcaagg tggctataag atttatgaaa cgactgtgcc gccaggcgct
     1681 ttcgtcattg atgatctgag tccgtcaggt tacggcagcg atcttattgt tactgtcgaa
     1741 gaatccgatg gctcaaagcg gacattctcg caaccttttt catcggtcgt tcaaatgtta
     1801 cgccctggcg ttggacgttg ggatattagc ggcggccagg tcttaaaaga tgatattcag
     1861 gatgagccta atttatttca ggccagctac tactacggtc tgaataacta tctaacgggt
     1921 tatactggta ttcagattac cgataataac tataccgctg gtttgttagg tcttggtctg
     1981 aatacttcag tcggtgcatt ttctttcgat gtgactcact ccaatgttcg tataccggat
     2041 gataaaacat atcagggaca aagttatcgt gtttcctgga acaagttatt cgaagaaaca
     2101 agtacttcac tgaatatcgc ggcctatcgc tattcgacac agaattacct tggtcttaat
     2161 gatgcactaa ctctaattga tgaagtgaaa catcccgaac aagatcttga accgaaatcc
     2221 atgcgtaatt actcgcgcat gaaaaatcag gttacggtca gtattaacca accgctaaaa
     2281 tttgagaaaa aagattacgg ttcattttat ctttccggaa gttggtccga ttactgggct
     2341 tccggacaaa atcgtagcaa ttactctatt ggctacagta acagtgcatc ctggggcagc
     2401 tacagtgtca gtgcccagcg ttcatggaat gaagacggcg acactgacga tagcgtttat
     2461 ctcagtttca ccattcctat tgaaaaatta cttggcactg aacaacggac ttcaggtttc
     2521 cagagtattg atactcaaat gagcagtgac tttaagggta ataaccaact caacgtcagt
     2581 agcagtggct atagtgataa cgctcgcgtc agttatagcg taaatactgg ctatacgatg
     2641 aataaagcca gcaaagattt gagttatgtt gggggttatg ccagctatga atcaccgtgg
     2701 ggaagcctgg caggttcagt ttctgcaaat agcgataaca gtcgtcaagt gtctctcagc
     2761 accgacggtg gttttgtatt gcatagcggc ggcctgacct tcagtaatga cagttttagc
     2821 gactccgata cactggcggt agttcaggct ccaggtgctc aaggagcgcg aataaattat
     2881 ggcaacagta ctatcgatcg atggggttat ggtgtcacca gcgctctttc tccttatcat
     2941 gaaaaccgta tcgcgctgga tatcaacgat cttgagaacg atgttgaatt aaaaagtacc
     3001 agtgcagtag ctgtaccgcg tcagggttca gtcgtctttg ctgattttga aaccgtgcaa
     3061 gggcaatcag ccattatgaa catcacacga agtgatggta aaaatattcc atttgctgca
     3121 gatatttatg atgagcaagg caatgtcatt ggtaatgttg gacagggtgg acaagcattt
     3181 gttcgtggta ttgagcagca gggaaatatc agcattaaat ggctcgaaga aagtaaaccc
     3241 gtaagttgtc ttgcgcatta tcaacaaagt tcagaagcag aaaaaatagc gcaatctatt
     3301 attctgaatg gaattcggtg tcagattcag taactacaag ggacgtcaaa tgataaaaat
     3361 aacgccacat aaaatagcga tactgatggg aatattatta tcaccatcag tatttgcaac
     3421 ggatattaat gtagagttta cagccactgt caaagcgaca acctgtaaca tcacacttac
     3481 tggtaataac gtcacgaatg atggcaataa taactacaca ttgagaatcc ctaagatggg
     3541 tctggataag atcgcgaata aaacgacaga atctcaggct gattttaaac tggttgctag
     3601 tgggtgcagc agtggcatca gttggattga taccactctg accggaaatg catcatcaag
     3661 ctcacctaag cttattatac cgcagtctgg tgattcatct tcgacgacaa gtaatatcgg
     3721 tatgggtttc aaaaaacgga ctactgatga tgccactttc cttaaaccta acagtgcgga
     3781 aaagatacgc tggagcacag acgagatgca gcccgataag ggtcttgaaa tgaccgttgc
     3841 gctacgtgaa acagatgcag ggcaaggcgt accggggaat tttcgtgcac tggcaacgtt
     3901 taatttcatc tatcaataag aaggataatt aagatgacat ttaaaaattt acgttatgga
     3961 ttatccagca gtgtcgtttt ggctgcctca ttgttcagcg taccctctta tgcggcaaca
     4021 gacagcattg ggctgaccgt gattactacc gtagaaatag gtacttgtac tgctacatta
     4081 gtaaatgact ctgatcagga catttctgtt gttgactttg gcgatgtata tatttctgaa
     4141 atcaatgcca agaccaaagt taaaacattc aaactcaaat tcaaagactg tgcgggtatc
     4201 cccaataaaa aagcacaaat aaaattaacc aagcgagcca catgcgaagg tactgctaat
     4261 gacggtgcgg ggttcgcaaa tggatccaca gccgcagata aagcaagtgc cgtcgccgtt
     4321 gaagtctgga gcaccgaaac tccggcaaca gggagcgcaa cacaatttag ttgtgtgaca
     4381 ccagcatctc aagaggtcac tatctccaat gcagccaacg cggttgttta ttatccgatg
     4441 agtgctcgac tggtcgtcga aaaaaataaa accgtaagca atgtcactgc gggtaaattt
     4501 tctgcacctg ccacatttac agtaacctat aactaacaga ctgacagatc caggaactat
     4561 atatgcatcc cactcaacgt aagctgatga agagaataat tctgtttctg tcactacagt
     4621 ttcccattac ctatccagcc atcgctgggc aggatattga ccttgttgcc aatgtaaaaa
     4681 acagcacttg caaaagcgga atcagtaacc agggtaatat tgatcttggc gtcgttgggg
     4741 ggggatattt ttcagataat gttactcctg aaagctatca accaggtgga aaagagttca
     4801 ctgtcacagt atcagactgt gcattacagg gaactggcga tgtgcttaat cagttacata
     4861 ttgattttag agcccttagc ggtgtcatgg ctgcaggttc aaggcaaata tttgccaatg
     4921 aagtcgcaac cggggctaaa aatgtcggtg tcgttctctt ttccattcag gacccgacca
     4981 atatattcaa tgttattagt tcagcaggaa attctcgttc cgtgtatcca gtaatgactt
     5041 ctgccctgca taattcttca tggaaatttt atgccagaat gcaaaaaatc gatcccgcgc
     5101 tggatgtcat atctggccag gtaatgagtc atatattagt tgatgtgtac tacgaataat
     5161 agatcaaaac aaataactgc gactgttaca gcagtagatg gtggagtgat aatgaagaaa
     5221 ttcttcagac attttctttt tttaattctg tgcttgagtt gttataccgc gagtgctggc
     5281 actgatgata acgtaggata tattgttggg aatagttacg gcgttggtcc gtcagatcaa
     5341 aaatggagag aaacggggcc gaatggcgat gctactgtca tattcagata tgcaacaagt
     5401 acaaataatt tagttttcta caaaccgacg cagcttggac ctacaggtgt aaaattacag
     5461 tggagtcagt tagataccgc ttctggtggt ggttttcttt attgcaacag aagtgatagc
     5521 acaagtggta gcgcaatgcg tattgaaaat gcaatggttg actcaggtaa aatgtat
//
LOCUS       DAPGWD010000222         5434 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPGWD010000222 DAPGWD010000000
VERSION     DAPGWD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.224"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1487)
                     /locus_tag="RHX88_004182"
     CDS             complement(<1..1487)
                     /locus_tag="RHX88_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDV2232794.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVSLLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSSG
                     QAASSAQSATSSAGTASTKATEASKSAAAAESSKSAAATSASAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSASSSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNA"
     gene            complement(1552..2151)
                     /locus_tag="RHX88_004183"
     CDS             complement(1552..2151)
                     /locus_tag="RHX88_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDV2232795.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(2222..>5434)
                     /locus_tag="RHX88_004184"
     CDS             complement(2222..>5434)
                     /locus_tag="RHX88_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV2232796.1"
                     /translation="YDTPITRTITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLV
                     QIQRNGGWVTEKDITIKGKTTSQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKT
                     LWSSYTEIIDVKQGYPNTALVGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQ
                     YSGIWDGTFKPAYSNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVP
                     DGFGGTEPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTY
                     NRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRN
                     VTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYA
                     GISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPASVEVQSVTDGLKVK
                     VNRVPDGVAGYSIWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGA
                     HFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAAD
                     DGSERLVSTARTTETTYRFTQLALGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPSR
                     IELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASI
                     NIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQI
                     DNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAV
                     KLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQI
                     FMNEVFLKRLTAPTITSGGNPPVFSLTPDGRLTAKNADISGSVNANAGTLNNVTVNEN
                     CTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIP
                     PIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPS
                     GRGSVTLEFKVFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT         1023 a         1652 c         1415 g         1344 t
ORIGIN      
        1 gcattgtcat aggctgattt tactgccttt ggcgttgccg ccagcgtttc agacgtactg
       61 ttggtcgcac tgctgagctg tactatcccc tttttcgtcg tacttgcatc ctcaagcgcc
      121 acggcggatg caatatcctc tgcccgtttt gccgctgtct cggcgcgcgt tgccgcggat
      181 tccgccgtac ttttgctctg tgctgccgcc gtcgcactgc cagctgcctc tgtcgccttc
      241 gtggatgccg tcgtggcgct acccttcgct gctgacgcct gtctggtcgc ctcatctttt
      301 gaagcagacg cagatgatgc cgatgacgac gccgaactgg ctgacgatgc ggcagccgtt
      361 tttgaggatt ctgcgctggt ttccgacgct ttcgcgttcg tttcggatgt cttcgctgcg
      421 gaagcagacc tcgctgctgc gctggcctgt tcagtggctt cgccagcctt cgttgtggct
      481 gttgaagcag acgatgcggc gctttctgcc gattttccgg cggcggtggc actggctgag
      541 gcctgcccgg cacttgttga cgctgcactg gcagacgacg cagccgctgt ttttgagcct
      601 gccgcagccg aggcgctctg tcccgctgcc gtttcagaag acctggcgtt cgtctctgac
      661 gtttttgccg ccttcgcgga atttcctgcc gctgttgccg aggaagctgc gctactggcg
      721 ctcgaggatg cgttcgtttc tgatgatttt gccgcctctt ttgaagccgc cgcatcccgg
      781 gctgaggtgg cagcttctga cgctttcgta gtcgcggtgg atgcagaagt ggctgctgat
      841 tgttgtgacg ctgcagcatt cgtttctgac gttttcgcgg cactggcact ggtagctgcc
      901 gcgctttttg aggactctgc agcggcagca ctttttgatg cttcagtggc ctttgttgat
      961 gccgttcctg cgctggaagt cgctgactga gccgacgacg cggcctgtcc ggatgacgtg
     1021 ctggctgcgc gtgctgagcc tgcagcatca gtcgcatggg ttgccgcctc acgggctgat
     1081 gtgctggcat cactggctga cttcttcgcg gctgccgtgt tctgtgccac cacggacgcg
     1141 ttacgcgcca cctcttccac catcagctca aaacggcgca gtgcctccgg acgggcatca
     1201 tcctccgtca tggcaccgag aaaatcattc agcgtaccgg gtcgggaatc ttcatacact
     1261 gtgatggtcc cggcatgtga cggcgggaat ccttccacca acagactgac gctgtactga
     1321 ccgtactcaa cgtccatact gtaacgcccg gcttcatccg gattttcaga tgccagcgtg
     1381 ttcaccacca ccgtggtact gttacgtttt gctttcagct ggattgtgca gttctgtacc
     1441 ggttttcctg tgccgtcttt cagtacacct gaaattttta ctgccatatt caccccacaa
     1501 aaaagcccgc ctgaaccggc gggctgtcat aacactgtgt tacctggcta atcagaattt
     1561 ataaccaaca cccacgatga acccgtcagt gcgccagtca ccactgccgg agccttcata
     1621 agcgacatca atggccacgg attcggtcgg gttaaactgc acgccagctc cccacgccag
     1681 agacgtgttg ctgtggcgac cgtcatcact tccggtcagc acatcgtgct ttttcccctt
     1741 gttgtcagtt acgcggagat aatccccgga gaaagtcgac acacggctgt aagccacacc
     1801 cgccatcgca tacgcgctga accattcatt cacgcgcaca gacggccccg ccattacgct
     1861 gaaccagcgg ttacgaacgg aatcttcatg ccagcgggta tcgctgtaac gggtaatctg
     1921 gcgattcttg tctcctgcat agctgaacga cgtcaccatc cccagtgtgt ccgtaaactc
     1981 ataacggtat ttcacgttaa tcccgttcag atcatcactg ccgggaacgt tcgtccgggc
     2041 atgaagatac cccgcgctca gcgtggactg atgttcagac gcccatgcag gcgcaccgga
     2101 tacggccaga caaatggctg cggacaaaat ggcggcataa agtttacgca taattacctc
     2161 tcgcttttct gcaataaaaa aggcgtcatt cctgacgccc tttattgggg ttataaatat
     2221 ttcaacgaat actgatgccg gaagcggctt ttttggtcac aatcaccgta cagtcggtga
     2281 tattgcctgc cccctgattg cctttctgga aaaccttaaa ctccagagtg acgcttcccc
     2341 tgccactcgg catatcaata accgcactgt agctaccggg aatggcccct ttagtttctc
     2401 tggatgcgat taatacgccg tttttgcgaa cttcaaaacc ataacccgtg tatcttgtac
     2461 ctcccgggtt attaccactt cccggatcgc tatacgctat tccgttaaag ataatgggcg
     2521 gaataatgat ttgacggtca aagttatgat catcgctgat ggtgactgta accgtcccgt
     2581 ttggtgtttc cgtattaccc cacgtaccag cctgtttcgg gaatgatttg gatacagctt
     2641 taacgaagtc acctctgacc tgagtcgcct ccagcatgcc cttaatcgta cagttttcat
     2701 ttaccgtgac attgttgagc gtcccggcgt tcgcattcac actgccactg atatcggcat
     2761 ttttcgccgt cagccgcccg tccggtgtca gggaaaatac cggaggatta ccgccgctgg
     2821 taatggtggg agccgtcagg cgtttcagga acacttcgtt catgaatatc tgatcgccct
     2881 gcccaacaaa catcggcttt gtgttgccat tcgcaggatt aatcatcgca atcctgtctg
     2941 ccgccagcag cacctgactc tgcatgccgt caggggtgtt ctcaataccg gcaccaatac
     3001 ccgcgatata aaggcgtccg tcctgcatct gctgcagctt cacagcccac atgctgttca
     3061 ggttattatt tgtatcaacc tgaaccttct gtatctgctg aattgccgca ctctggtctt
     3121 ccagtttctt attgacggtc tgcgtgattt cattgctgac atccgtaatg gacgtcctga
     3181 tttcagccag gtcaggcgca agctgaccgt tatcaatctg cgtccacagc tcctgagcca
     3241 gatgggtttt ccctatctcg cctttgaaaa aatccagata accggatgca tcatcactcg
     3301 gctgaccaac agcctccacg aatgccgatt tgccaacggt gttcacactg cggatataaa
     3361 agtaataatc atggcccggt ttgatattga tactggcggc tatccagtac agcgccgtgc
     3421 caagatagcg tgctgtggtt tcaacctgcc tgatatccgc aatccgcttt tccgagaacc
     3481 agaactcaaa ctgtaccgtc gggtcataaa cggcaagatg tggcgtggcg gttatctgaa
     3541 aatagcccgg cgtcagctca atcctcgacg gtgctgccgg tgcggcaatc cggaacgata
     3601 ccgacgccgg atcgccctgc tgtccccagg catttgccgc ccggactgtc agcctgtagt
     3661 ttcccagcgc cagctgcgtg aagcggtatg tggtttccgt cgtccgggcc gtgctgacca
     3721 gccgctcact gccgtcatcc gctgccacgg tcaggcgaag cataaagctc acacccttca
     3781 ccaccttcgg cgtgtcccag cgcgccagca cctggtattc accgctgtct gcggtgactt
     3841 ctgcggtcag gtgctgcact gctggcggcg tgacaccgtt taccgttccg ctctggtcgc
     3901 cgtcaaagtg cgccccgtta tccacgatgg cttctttctc cggtacatgc tgcacggcag
     3961 tgatggcata cgtgccgtca tcgttctcac ggatactcac acagcggaac aggcgctggc
     4021 gcaacgtcgg cagcttcagc ccccatatgc tgtatccggc aacgccgtca ggaacccggt
     4081 tcactttcac cttaagtccg tcggtgacgg actgaacctc cacgctggcc ggattgccac
     4141 ttccgtcaac caggcttatc agcgtggtgc cggaggatgg cagcgtgatt tcacggtcga
     4201 gcgtcagcgt ccgggtctgg ctgttcaccg ccagcacgcg accaccggtg ctgataccgg
     4261 catagtcatc atcgcagatt tcaatgacat cgcccggtac atggcgaagc ccttcagcac
     4321 ccacgctgaa atccacggtc tgcgtctcca gcagttccgt tttaatcagc cacagcccgg
     4381 cgcggtgtgc ctgcccccgg ctggtacagc caaaggcatc catcttcgtg acattacgac
     4441 cgtaacgggc aatggcctgc gtatcttcaa caagctctgt cgccgtctcc cagccgttgt
     4501 ccgggtcaat ccagttcacc tcaacggcat tatggcggtc cttgagggcg ctgaagctgt
     4561 agcggaacgg cgcgccatca tccggcatca ccacattact gcggttatag gtccacacct
     4621 tatccgacgg tcggtcctgc acgaacgtca gcgtctgccc gttccatacc ggcatacagc
     4681 gcatcgccga gcagaaatca ctgagcacat cccacgcctt acgctgtgtg gtcaggtacg
     4741 cattacaggt gatgcgcggc tccgtgccgc caaagccgtc cggcactgac tggtcgcagt
     4801 actggccgat gacatacagc gcccatttgt ccacatccgc cgcaccaaga cgtttcccca
     4861 tgccgtagcg tggatgggtc agcatatccc acagacacca ggccatgtta ttgctgtatg
     4921 ctggcttaaa cgttccgtcc cagataccgc tgtattgccg cgtctgcggg ttatagttcg
     4981 acggcacctg cagaatacgc ccgcgcagat gataattacg gctcacctgc tggctgccga
     5041 actgctccga gtccacctgc acgccgacca gtgccgtgtt cgggtagccc tgtttcacat
     5101 cgataatttc ggtgtatgac gaccagagcg ttttgttctg cagctggtct gtggtgctgt
     5161 ccggcgtcat cctgcgcatc cggatattaa acgggcgcgg cggcaggtta tccaccacca
     5221 cagaggccag gtactgcgag gtggttttgc ctttaatggt gatgtctttt tccgtcaccc
     5281 agccaccgtt acgttgtatc tgaaccagca ggcggacttc cgatggattc ctgtccccct
     5341 ttgaggtggt ttccaccagt gcctgcacgc cgaaggtaaa acgcagtcgg tcgatgtttg
     5401 ccgacgtgat ggtccgggtg atcggcgtgt cgta
//
LOCUS       DAPGWD010000223         5332 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPGWD010000223 DAPGWD010000000
VERSION     DAPGWD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.225"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            35..1237
                     /gene="fes"
                     /locus_tag="RHX88_004185"
     CDS             35..1237
                     /gene="fes"
                     /locus_tag="RHX88_004185"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDV2232797.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            1240..1458
                     /gene="ybdZ"
                     /locus_tag="RHX88_004186"
     CDS             1240..1458
                     /gene="ybdZ"
                     /locus_tag="RHX88_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDV2232798.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            1455..>5332
                     /gene="entF"
                     /locus_tag="RHX88_004187"
     CDS             1455..>5332
                     /gene="entF"
                     /locus_tag="RHX88_004187"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDV2232799.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQVLML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLN"
BASE COUNT         1146 a         1409 c         1571 g         1206 t
ORIGIN      
        1 atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga
       61 gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga
      121 ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt
      181 atggatctac atcactggtg tgaccgatca tcatcagaac agccagcccc agtcgatgca
      241 gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca actggcgcgg
      301 cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat cccccgatcg
      361 cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg atccgctgaa
      421 tccacaaagc tggaaaggcg ggcgagggca cgctgtttct gcactcgaaa tgccgcaagc
      481 gcctctgcaa ccgggatggg attgtccgca agcgccagaa atagcagcca aagaaattat
      541 ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca ccggcgatgc
      601 aacagcagaa gaacgtccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat
      661 gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta
      721 tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga
      781 tttctggctg gcagtacagc aagagttatt acccctggtg aaagctattg ccccttttag
      841 cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta
      901 tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg
      961 gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctta aagctggtga
     1021 agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgattat
     1081 gcgggccaat caggcgctgt atgcgcaatt acaccccata aaagaatcca ttttctggcg
     1141 tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggctaat
     1201 cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc
     1261 gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg
     1321 ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaggcg
     1381 tcctgccagc agtggctgga agtccactgg cgtactctga caccggccaa tttcacccag
     1441 ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga
     1501 tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa
     1561 ctggagatgt tgatgcgcca ttactggccc gcgcggtggt tgtcggacta gcgcaagcag
     1621 atacgttgcg gatgcgtttt acggaagata acggcgaagt ctggcagtgg gtcgatgatg
     1681 cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat ccgcacggta
     1741 ctgcgcaggt attaatgctg gcggatttgc aacaagattt gcgcgtcgat agcggtaaac
     1801 cactggtctt tcatcagctg atacatgttg cggataaccg ctggtactgg tatcagcgtt
     1861 atcaccattt gctggtcgat ggcttcagtt tcccggccat tactcgccag atcgccaata
     1921 tttactgcac atggctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg
     1981 atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat
     2041 tctgggcaga acagcgtcgt caactgccgc cgcccgcgtc actttctccg gcacctttag
     2101 cggggcgcag cgcttcggca gatattctgc gcctgaaact ggaatttacc gacggggaat
     2161 tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctgg
     2221 cagccttgtg gctggggcga ttgtgcaatc gcatggacta tgccgccgga tttatcttta
     2281 tgcgtcgact gggctcggcg gcgctgacgg ctagcggacc cgtgctcaac gttttaccgt
     2341 tgggtattca cattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcagcac
     2401 aactgaaaaa aatgcgtcgt catcaacgtt acgatgccga acaaattgtc cgtgacagcg
     2461 ggcgagcggc aggagaggaa ccgctgttcg gtccggtact caatatcaag gtatttgatt
     2521 accaactgga tattcctggt gttcagacgc aaacccatac cctggcaacc ggtccggtta
     2581 atgaccttga actggccctg ttcccggatg aacacggtga tttgagtatt gagatcctcg
     2641 ccaataaaca gcgttacgat gagccaacgt taatccagca tgctgaacgc ctgaaaatgc
     2701 tgattgccca gttcgccgcg gatccggcgc tgttgtgcgg cgatgtcgat attatgctgc
     2761 caggtgagta tgcgcagctg gcgcagatca acgccactca ggttgagatt ccagaaacca
     2821 cgctgagcgc gctggtggca gaacaagcgg caaaaacacc ggatgctccg gcgctggcag
     2881 atgcgcgtta cctgttcagc tatcgggaaa tgcgcgagca ggtggtggcg ctggcgaatc
     2941 tgctgcgtga gcgcggcgtt aaaccagggg acagcgtggc ggtggcacta ccgcgctcgg
     3001 tctttttgac cctggcactc catgcaatag ttgaagctgg cgcggcctgg ctaccgctgg
     3061 ataccggcta tccggacgat cgcctgaaaa tgatgctgga agatgcgcgt ccgtcgctgt
     3121 taattaccac cgacgatcaa ctgccgcgct ttagcgatgt tcccaattta acaagccttt
     3181 gctataacgc cccgcttacc ccccagggca gtgcgccgct gcaactttca caaccgcacc
     3241 acacggctta tatcatcttt acctctggct ccaccggcag gccgaaaggg gtaatggtcg
     3301 ggcagacggc tatcgttaac cgcttgcttt ggatgcaaaa ccattacccg cttacaggcg
     3361 aagatgtcgt tgcccaaaaa acgccgtgca gttttgatgt ctcggtgtgg gagtttttct
     3421 ggccgtttat tgccggggcg aaactggtga tggctgaacc ggaagcgcac cgcgacccgc
     3481 tcgctatgca gcaattcttt gccgaatatg gcgtaacgac cacgcacttt gtgccgtcga
     3541 tgctggcggc atttgttgcc tcgctgacgc cgcaaaccgc tcgccagagt tgcgcgacgt
     3601 tgaaacaggt tttctgtagt ggtgaggcct taccggctga tttatgccgc gaatggcaac
     3661 agttaactgg cgcgccgttg cataatctat atggcccgac ggaagcggcg gtagatgtca
     3721 gctggtatcc ggcttttggc gaggaactgg cacaggtgcg cggcagcagt gtgccgattg
     3781 gttatccggt atggaatacg ggtctgcgta ttcttgatgc gatgatgcat ccggtgccgc
     3841 cgggtgtggc gggtgatctc tatctcaccg gcatccaact ggcgcagggg tatcttggac
     3901 gacccgatct gaccgccagc cgttttattg ccgatccttt tgccccaggt gaacggatgt
     3961 atcgtaccgg agacgtcgcc cgctggctgg ataacggcgc ggtggagtac ctcgggcgca
     4021 gtgacgatca gctaaaaatt cgcgggcagc gtatcgaact gggcgaaatc gatcgcgtga
     4081 tgcaggcgct gccggatgtc gaacaggcag ttacccacgc ctgtgtgatt aaccaggcgg
     4141 cagccaccgg tggtgatgcg cgtcagttgg tgggctatct ggtgtcgcaa tcgggactgc
     4201 cgttggatac cagcgcatta caggcacagc ttcgcgaaac attgccgccg catatggtgc
     4261 cggtcgttct gctgcaactt ccgcagttgc cgcttagcgc caacggcaag ctggatcgca
     4321 aagccttacc gttgcctgaa ctgaagacac aagcgtcggg acgtgcaccg aaagcgggca
     4381 gtgaaacgat tatcgccgcg gcattcgcgt cgttgctggg ttgtgacgtg caggatgccg
     4441 atgctgattt ctttgcgctt ggcggtcatt cgctactggc aatgaaactg gcagcgcagt
     4501 taagtcgaca gtttgctcgt caggtgacgc cggggcaggt gatggtcgcg tcaaccgtcg
     4561 ccaaactggc aacgattatt gatggtgaag aggacagctc ccggcgcatg ggattcgaaa
     4621 ccattctgcc gttgcgtgaa ggtaatggcc cgacgctgtt ttgtttccat ccggcgtccg
     4681 gttttgcctg gcagtttagc gtgctctcgc gttatctcga tccacaatgg tcgattattg
     4741 gcattcagtc gccgcgccct catggaccca tgcagacagc gacaaacctg gatgaagtct
     4801 gcgaagcgca tctggcaacg ttacttgagc aacaaccgca cggcccttat tacctgctgg
     4861 ggtattccct tggcggtacg ctggcgcagg gtattgcggc gcgactgcgt gcccgtggcg
     4921 aacaggtggc atttcttggc ttgctggata cctggccgcc agaaacgcaa aactggcagg
     4981 aaaaagaagc taatggtctg gacccggaag tgctggcgga gattaaccgc gaacgcgagg
     5041 ccttcctggc agcacagcag ggaagtactt caacggagtt gtttaccacc attgaaggca
     5101 actacgctga tgctgtgcgc ctgctgacga ctgctcatag cgtaccgttt gacggtaaag
     5161 cgacgctgtt tgttgctgaa cgcacacttc aggaaggtat gagtcccgaa cgcgcctggt
     5221 cgccgtggat agcggagctg gatatctatc gtcaggattg tgcgcatgtg gatattatct
     5281 ctccaggggc gtttgaaaaa attgggccga ttattcgcgc aacgctaaac ag
//
LOCUS       DAPGWD010000224         5180 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPGWD010000224 DAPGWD010000000
VERSION     DAPGWD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.226"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..83)
                     /locus_tag="RHX88_004188"
     CDS             complement(<1..83)
                     /locus_tag="RHX88_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDV2232800.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALE"
     gene            complement(141..1160)
                     /locus_tag="RHX88_004189"
     CDS             complement(141..1160)
                     /locus_tag="RHX88_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDV2232801.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADRAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(1172..2386)
                     /gene="rspA"
                     /locus_tag="RHX88_004190"
     CDS             complement(1172..2386)
                     /gene="rspA"
                     /locus_tag="RHX88_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDV2232802.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLELFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2592..2918)
                     /locus_tag="RHX88_004191"
     CDS             complement(2592..2918)
                     /locus_tag="RHX88_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDV2232803.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            3053..3394
                     /locus_tag="RHX88_004192"
     CDS             3053..3394
                     /locus_tag="RHX88_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDV2232804.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3429..3989
                     /gene="speG"
                     /locus_tag="RHX88_004193"
     CDS             3429..3989
                     /gene="speG"
                     /locus_tag="RHX88_004193"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDV2232805.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3992..4702)
                     /locus_tag="RHX88_004194"
     CDS             complement(3992..4702)
                     /locus_tag="RHX88_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDV2232806.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4810..5115
                     /locus_tag="RHX88_004195"
     CDS             4810..5115
                     /locus_tag="RHX88_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDV2232807.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1408 a         1307 c         1132 g         1333 t
ORIGIN      
        1 aattcaagcg cagtgcccag ccatcccgat actgctgctt tcaccaaatc cttagtgctt
       61 ctttcatgtt tttctattgt cataatggtt atctctaaaa aagaggtaag atgcgtacta
      121 cttactcgcc gttattggta ttattcagaa aaagtgagta agactttgca gcaatgtttt
      181 tgatcctgtt caaataaact aatggcatca gcaacatgct ggaaatcaaa cgtatgggta
      241 attaattttt ctggtttaat taacccttta cttaaccagt cgataacaac cggaaattta
      301 tttgcattta agcgtgaaga gaaaatagag agttcttttc cggtaattcc ttgctgaatc
      361 acttcagacg gttcactgga gaagcccatc aatacaatac gtgccgctgg agaagccagc
      421 gttacggcct ctttcaggat agaaggatga caagccgcat cgataattaa tgtcggcttg
      481 atgccttttt cagcgaaaat ctcgccaagc ggtgtctggc tgttattaat cgccctgtct
      541 gccccgctct ctttcgcttt ttccagtcgt tcatcaatgc gatcggcaac aatcacattt
      601 ttaacgttat agacgccttt taatacctga acgatcgtca ggccgattgg accggcacca
      661 taaaccagaa cggtatcatt ttcagtcggt tgaccatgtc cggttacgtt agccgcaatg
      721 gtaaaaggtt cgatcatcac cgcatattga tcggccactg cttcaggaat tttccacgca
      781 ttttttgccg gaaccacggc atattcactg aaaccaccgt cagcgtgcac acctaataca
      841 gcaagtgtcg tacaaacgtt cggtttacct atagagcacg gatagcaatg cccacagctg
      901 accaccggat cgacagcaac acgttcgccg actctggcgc tttccacgcc gtcacccacc
      961 gcatcaatga cgccaaagaa ttcatgtcca atgacgcgcg gatatttcgc aaaaggatta
     1021 tgcccacgat aaatatggct atcagaacca caaattccgg caagtttcac ttttactcgt
     1081 acttcacccg ctgacggggt gggtatttca cgttcgacaa tcgccagttg attcggtttt
     1141 tcaattaata tgcttttcat tatcttactc cttaccagtt ccacagcgtg ccatcttcca
     1201 gacgtgcgac tggtagataa gcaggttcat agggatattt cgccgccagc ttttcatcga
     1261 attcgatgcc aagacccggt ttgtctcccg gatgcatata gccgttatcg aaagtccagt
     1321 tgtgcgggaa gagttcgagc atttgttcgg aataacccat gtattcctgg acaccgaaat
     1381 tggggaccca cagatcaaag tgcagcgctg cagccatgca gactggtgac aaatcggaag
     1441 gaccgtgtga gccagtacgt acctgataca gcgaagcaaa atcggcaatc cggcgcatac
     1501 cggtaattcc gcctgcatgg gtcagcgtgg tgcggatata atcgatgagt tgctcttcaa
     1561 tcagttgttt gcagtcccag atgctgttga agacttcacc cactgcgatg ggtgtgacgg
     1621 tatgttggcg aatgagacgg aagcattcct ggttttctgc aggcgtcggg tcttccatcc
     1681 agaacatgcg ataatcttca atgcttttac caaagcgcgc cgcttcaata ggcgttaagc
     1741 gatggtgcat gtcatgcagc aaatgttcat taaaaccaaa cttgttacgt accgcgtcaa
     1801 acaatttcgg catgaaatcg aggtatttct ccgtcgacca cagctgctct tccggccact
     1861 gtcctttggt tgcgggttca taagccagac ctttaccttt cgacatgccg taggtggttt
     1921 tcataccagg gattccgcac tgcacgcgga tggctttgaa tcccagctcc tgatgacggg
     1981 cataatcatc cagagcttca tcaatactgt gaccggtggt atggcaataa accatcaccc
     2041 cttcacgaga cgcgccgccg agtaactggt aaagcggcat gttggcagct ttggctttaa
     2101 tatcccacag cgccatatca accgctgaaa tggccgacat cgtaaccgga ccgcgacgcc
     2161 agtaagcacc tttatagaaa aactgccaga tatcttcgat acggtgcgca tcgcgaccaa
     2221 taagctgcgg acaaaggtga tcctgcaaat aagaggccac ggaaagctca cgtccattga
     2281 gggtggcatc cccaaggccc gtaataccgt cctcagtggt gatttttaat gtgacgaaat
     2341 tacgccccgg acaggtaaca aaaacttcag cctttacgat cttcatgttc gattccttgc
     2401 atcgcttgtc gtgatgcatg aaatctacgc aactgagcta ctaccataca agtatgaaga
     2461 tcgaaaaaag ccggagtgat cacaaaaaaa ggcgtatatt tgcgctgtga atggttgaca
     2521 aaagatgaaa tagaatacct tttgtcagct gacacttcct cttatcttat tgataaaatg
     2581 gatttatgtt cctacgtgcg cccccagccc gcaacaatga tcaacatgcc gcaaagcgca
     2641 atcaacgcac ccgtccagtc ataaagagtc agtttcacgc catccacaac gcgcagccac
     2701 atcaacgccg tgcagacata aacgccacca taagccgcgt aaacacgccc actcgccgct
     2761 ggatgcaacg ttaacaacca gacaaacagc gccagtgaaa tccccgccgg aagcaacagc
     2821 cagatactgg cgtttcgttt taaccacaac cagggcagaa agcatccaat aatttcacac
     2881 agcgcagtag caaaaaatag taacgttgtt ttaatcatct ttgtcactta ttgacatcat
     2941 gtatagttat agggcgacat aatatcatca atataaacac cctcctggta cgttttgcgt
     3001 tcgcagtgga tggtgtagaa tctcctttaa tcattcatac agggaatgaa ttatgaaaat
     3061 cactctcagc aaacgaatcg gcctgctcgc ttttctgctg ccttgcgcac tggcactgag
     3121 cacaactgtt catgccgaaa ctaacaaact ggtgattgag tctggcgaca gtgcacaaag
     3181 ccgccagcac gccgctatgg aaaaagagca atggaatgac acgcgcaatc tgcgtcagaa
     3241 agtgaataaa cgcactgaaa aagagtggga taaagccgac gccgcttttg ataaccgcga
     3301 taaatgtgag caaagcgcca acatcaatgc ctactgggag ccaaatactt tgcgctgcct
     3361 ggaccgtcga actggccgcg ttattacccc ctaacctgtt attgatttaa ggaatgtaag
     3421 gacacgttat gccaagcgcc cacagtgtta agctacgccc gctggagcgt gaagatttac
     3481 gctatgtaca tcaactcgac aataacgcca gtgtgatgcg ttactggttt gaggaaccct
     3541 acgaagcctt tgttgaactc tctgatctgt atgataagca tattcacgat cagagcgaac
     3601 gacgctttgt ggtggaatgt gacggcgaaa aagccggtct ggtggagctg gtggaaatta
     3661 accatgtaca tcgccgggca gaatttcaga taattatctc cccggagtat caggggaaag
     3721 gtctggcaac ccgtgccgcc aaattagcaa tggactatgg cttcaccgtt ctcaatctct
     3781 ataagctgta tctgatcgtc gataaagaga atgaaaaagc gattcacatt taccgcaagc
     3841 ttggctttac ggttgaaggt gaattgatgc acgagttctt tattaatggt caatatcgta
     3901 atgccattcg catgtgtata ttccagcatc agtatctggc agagcacaaa acaccgggtc
     3961 agactctcct gaagccgacc gcacaatagc attaataata atcgatcgta tttttgatgg
     4021 tgtaaacccg ttcgacggcg ggttttactc cttcatcaac aatgatcagc tgacagtcca
     4081 ccggattggc gtgactgtca tattcacagc tctgttttac attaccaact cgttgattat
     4141 tcagtaaagt aaccgctgtg taatctaatt ttttgatcgg atccgttgat ggcgtggcgc
     4201 tgacagataa tgttttgtcg ttacttttcg tggttttacc cagcggataa ccctgatcat
     4261 catagcgata ttccatctgc atttgtttgc tgctggcttt aatcacgaag ccattatcat
     4321 cggtttccca actcaccccg gcagaaggta attctgctag ctggcatttt ccctgtaaac
     4381 gtactctctt ctccagcgtc tcggcatcac ggtaataatt ggcgtccagt accagagcga
     4441 ccacggtatt attttccaga tccagtaatt cgagtgaatc aaaacagcct tcttccgaca
     4501 aagtcccaga aacacgtttc gtcacttcac cttgctcatc cattaatgtc tgagtgaaat
     4561 cttttaccgg accacgcagc ggatcaaaat cgaattcatt agagaaactg gccatctcag
     4621 gggtaaatga aagtgttacc tctgtgcggt cacatcctgt gaggaatatc gcgagtaagc
     4681 atggtagtaa tttgtatttc acaacagtca ccagagagta gagatgattc tcaatcatag
     4741 tagcaaatac agtactttac acgttaaatg ctatgcttaa agaagttatc ttcgcgtaag
     4801 gagcttatga tgaaactctc aacctgctgt gccgcgcttc tgctcgccct cgcctcaccc
     4861 gtggtactcg ccgcgccggg atcctgtgag cgcatacaga gcgatatatc acagcgcatt
     4921 atcaataatg gcgtacccga aagcagcttc acgttaagta ttgtacccaa tgaccaggtt
     4981 gatcagcctg attcccaggt cgtcggccat tgcgctaatg atacgcataa aattctctat
     5041 acccgcacca ccagcggtaa cgtctctgct cccgcgcagt ctacccagga tggcgcgcct
     5101 gccgaaccgc agtaatacat tatcgactga acgccggata cgacacatcc ggcgatttga
     5161 acatacaaca taatcccacc
//
LOCUS       DAPGWD010000225         5168 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPGWD010000225 DAPGWD010000000
VERSION     DAPGWD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.227"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(260..451)
                     /locus_tag="RHX88_004196"
     CDS             complement(260..451)
                     /locus_tag="RHX88_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232808.1"
                     /translation="MAESRYGLAGLGIGKVKSINQYRLTPGFGGFTPVSYATTECRLP
                     CRWRGICWWLCLKKVIYSA"
     gene            complement(1157..2179)
                     /locus_tag="RHX88_004197"
     CDS             complement(1157..2179)
                     /locus_tag="RHX88_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV2232809.1"
                     /translation="MVIKNTRTNHWTQLVPEEQIRFWEDYEAGRATSFLVEPERNRTK
                     RRRGEHSTKPKCENPSWYRPARYKALSGQLGHAYNRLVKKNPVTGEQSLRMRMSRHPF
                     YVQKRSFAGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKDSKG
                     KVIRELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPHYVWITPAGWQMLGV
                     DMIKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHRRAK
                     AAARKRANRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKQLAIRELRQLELTL
                     AAPPPH"
     gene            complement(2169..2594)
                     /locus_tag="RHX88_004198"
     CDS             complement(2169..2594)
                     /locus_tag="RHX88_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDV2232810.1"
                     /translation="MGKAVGGTETFAFDIASACARANGKRKPSVLRRRAIDALLQGMC
                     FYYDPVSNQVHRSITELAFDCGLARKNTHGHLAIERAVRAIKSQEEDFGFIVCSPSSG
                     FYNKRCAITLTPRFFEFLGVFPLALTEARLAVLRSGYGD"
     gene            complement(2689..2763)
                     /gene="tap"
                     /locus_tag="RHX88_004199"
     CDS             complement(2689..2763)
                     /gene="tap"
                     /locus_tag="RHX88_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HDV2232811.1"
                     /translation="MPGKVQDFFLCSLLLCIVSAGWRG"
     gene            complement(2997..3252)
                     /gene="repA"
                     /locus_tag="RHX88_004200"
                     /pseudo
     CDS             complement(2997..3252)
                     /gene="repA"
                     /locus_tag="RHX88_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
     gene            complement(3901..4116)
                     /locus_tag="RHX88_004201"
     CDS             complement(3901..4116)
                     /locus_tag="RHX88_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232812.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGLVVILEVRGRWMEAAEAWLRAAC
                     VAAPRTNWQQFARKRAEHCHRRCRGRL"
     gene            complement(4245..4805)
                     /locus_tag="RHX88_004202"
     CDS             complement(4245..4805)
                     /locus_tag="RHX88_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDV2232813.1"
                     /translation="MAEQKRPVLTLKRKTEGEAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAVKKAQARQVLSIYLNLPTLNEAVNTLKPWWPGLFNGDTPRLLTYGIRE
                     ALLEDVVRRNIPLSHKKLCRALKAITRSESYLCAMRAGACRYDTEGYVTEYISQEEEA
                     YAVERLAKIRRQNRIKAELQAVLDEK"
     gene            complement(4908..>5168)
                     /locus_tag="RHX88_004203"
     CDS             complement(4908..>5168)
                     /locus_tag="RHX88_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232814.1"
                     /translation="PFLTVMETLQYKPAESAARVQCPVLVVIAGQDSVNPPEQGRALY
                     DAVASGTKELYEEADACHYDIYEGAFFERVAAVQTQWFKKYI"
BASE COUNT         1201 a         1356 c         1218 g         1393 t
ORIGIN      
        1 tatgggggaa attaccctat ataatttttg acgccaataa ataaaaatag ccgcatatta
       61 gcgctcctaa tgctgcagga ttgctcgatt attcatcacg ccatttaacc agaccgcagt
      121 atcgcttgtt ttttcaattt cctatacatg cccccctttc attcaataaa ggacaattgc
      181 aggttttttg atgccgcacg catgggtata acgagtcaga gtatcgatat tcgcctggta
      241 atttgcgtgt tgtctggttt tatgcagagt atataacctt tttcagacac agccaccaac
      301 atatgccgcg ccagcggcat ggaaggcggc actccgttgt tgcatatgat accggagtaa
      361 aaccgccgaa gcccggcgtc agccggtact gattgataga tttcacctta cctatcccca
      421 gccctgctaa gccatacctg ctttcagcca tgacagagct tttgtgcgcg gtcggagtgg
      481 tcccgacgag ggtttacccg aagtcggggc gtatctccgc gttagcgggc cgtcagggcc
      541 gcttacgagc gtgtactgag aacatccagc cagaagactg acagcgatga cgatgcttag
      601 ttacaacatt catagttaaa agcgactctg ttccggccct tcgggccggg gcggggccgc
      661 ttttcagtta tgagggaagg gctttgtggt ttcagttctg tgctggacct gggtttttcc
      721 gggatttatg tttgtatgtt gtaactaaac tagctccggt tggggcccgc cgcttgcggt
      781 gggaggtgct tttaaattct gagcggcgcg gctttggccg ctgcaagcga aggccttttt
      841 gtatttgttc acaggagtgt cagagataaa gactttccct gcggtggaac aacgcagggg
      901 attaaatgaa ggggttaaga ctatattgca ggtttgttgt ttctagattg cggcttcttt
      961 gagttctttg tttgcggatt tatgtggata actgaaacga cgtggccaca ttctgacgac
     1021 cggtgagtga ttttatggcc gtgtatggac ggatgtgggg atttcaggtg gttacggggg
     1081 atttctggcc ggacagaacc tgcgccaaaa gccccgaagg ggctggcgat gattcagtgc
     1141 tgaaggatgg tgctgtctag tgcggtggcg gtgcagccag cgtcagttca agctgacgca
     1201 actccctgat ggccagttgc ttcagatggt catcggaaca aaaataggct tcatccggcg
     1261 gcaggcgctt cctgagatac tcggccatct catgaatctg ctggtcggca ggtagcttct
     1321 taagacgatt agcacgcttg cgggctgcag cttttgcccg acggtgtttc agcgcatcct
     1381 ggctgcgctg cagataccag cgttttctgg ccgcatgtac ggagatgtct tcatcctcac
     1441 gcagtacgcc ttcccggata agctgctggc ggatttcact ttcacgcagc cgcttctgct
     1501 gctgttcgtg aagtttaatc atgtcgacgc ccagcatctg ccaccctgca ggtgttatcc
     1561 agacatagtg cggcagacgc tggcgggttt cacggtccca catcgtttct tccgacacac
     1621 ccagcacacc aaaacgcacc tgttcggcca gcagacggga aagccgggag accgtcacct
     1681 caagttcgcg gataaccttt cctttactgt ctttcgggct gatttcttta gccaggcgag
     1741 tgacactcat gcccacagta tgtgtgcccg catcactgaa gctgaccaga accggccaga
     1801 tagcatcgag gagacgttgt ttttccggcc ggaaggcata tttacggcct gcgaacgacc
     1861 gtttctgcac gtaaaagggg tgtcgcgaca tgcgcatgcg caggctctgc tcgccggtca
     1921 ccgggttctt tttcaccaga cggttatagg cgtgcccgag ctgcccgctc agcgccttat
     1981 agcgcgcagg acgataccag gacggatttt cgcatttggg tttagtggag tgctcaccgc
     2041 gacgacgctt tgtgcgattc ctctccggtt caaccagaaa agaggtcgcc cttcccgctt
     2101 catagtcttc ccagaaacgg atttgctctt cgggaaccaa ctgagtccag tggtttgtac
     2161 gggtattttt aatcaccata cccactccgt aagacagcca gacgagcttc agtcagcgcc
     2221 aacggaaata cccccaaaaa ctcaaaaaaa cgaggcgtca aagttattgc gcagcgctta
     2281 ttgtagaaac cggatgacgg ggaacagact ataaacccaa aatcttcttc ctgtgattta
     2341 attgctctga cagctcgctc aatggccaaa tgaccatgag tattttttct ggctaatccg
     2401 caatcaaatg caagttcggt tatagaacga tgcacttgat ttgaaacagg atcataatag
     2461 aagcacatgc cttgcaacag agcatcaata gctctgcgac gaagaacaga aggtttacgt
     2521 ttaccattag cacgcgcgca cgcagaggca atatcgaatg caaatgtttc ggtccccccc
     2581 accgcttttc ccattagctt ctcgcagaat ggctgcgtcc tggcaccttc acggggggtg
     2641 aacaccgaat tcgggttctt tacctgacgg gaatacgttt gttgaagatc agccacgcca
     2701 tcctgcactt acaatgcaca gaaggagtga gcacagaaag aagtcttgaa cttttccagg
     2761 catataacta tactccccac atagcttgat tgttggctat gcggttttat gggccccggt
     2821 aatcttctct tagtcgccaa acctgttgaa gattattggg gctttcgctt ttctggctcc
     2881 tgtaaatcca catcagaacc agttccctgc caccttatgg cgtggcctgc cagaaaattc
     2941 cttaaacgat cagtaaccta gcactaacag aaaaaatcct caagaatgtg cgcccatcat
     3001 atcatattcc ggcgacaggc ctctttctca ataagtcttt ctatcaattc tgcctgagtc
     3061 aggccttcct ttgcacacag acgggtaaga tagtttttca gctgttctga caaaaacctt
     3121 aatctcttta catacagctc gcttacgtgc aaaaacagct ctctgtcgct ctgcttcgct
     3181 caaaggttta tcctttctgt atgctcgttt tacgtcagat gaggaagtca ctgcattttc
     3241 tgtctgcgaa atctcacctc ctcaatgctc aaacagggag catttcgcag aggatactac
     3301 agttttttga aatcagcgac ttaagaattg tgacgaagat ctgtgttggc gcacaaatca
     3361 tgtcggatta cgagcgttta atgtgatttt gatgatgaaa tgattaaaaa aacagaaaat
     3421 accttgacct gggtgccagc cccggacggg accagctatt tctggcatgt cgggtgtcac
     3481 cccctgcaat acctaacatt ttgggaaagc cgggacttcc ttctccggcc ggcagcttac
     3541 ttctgtgttt cgtaggccag attatctgta ccactgtatt tcccctgtgg atattcagct
     3601 cacttgcccc agggttttac gatgcaccgg ataattccag tagaatatag ttcctcatat
     3661 tattctccat atcaaataaa tgacacctgt atttcaggca tcctgaatat tgcagtacac
     3721 attgaagaac acattatttt gtgaaattag tcacaaaata taaaagcgcg acttaatacg
     3781 gaaaaaactc tgtgctactc agaactacct ggtcatactg cccgaccgga tataaaaagc
     3841 aaatctcata aactcctttc agccttcaga ctcctggagt aatgaaggct gcttttttcg
     3901 ttacagcctt ccccggcagc gcctgtggca gtgttcagcc cttttccggg caaactgctg
     3961 ccagtttgtc cggggcgccg caacacaggc agcacgtagc catgcttcag cagcctccat
     4021 ccagcgcccc ctgacttcca gaataacgac cagccctccg gcacgctgat atcgccagac
     4081 ctgaccgttc cgtgaactgt tcctgaatcc attcattttt ctgcccctcc cttttagtcg
     4141 tcagtattga gttaaataat caggggactg gcttccttac gggtacggaa gttacttcct
     4201 tttcggcctt atatctttgc ctcctgtctc tgcgggaggc ttttttattt ctcatcaagc
     4261 acagcctgaa gctctgcttt tatccggttc tgtcggcgga ttttcgccag acgctccacc
     4321 gcatacgcct cctcctcctg tgagatgtac tccgtgacat acccctcagt gtcataccga
     4381 caagcaccgg ctctcatggc acacagataa ctttccgaac gggtaatggc tttcagagca
     4441 cgacacagtt ttttatgcga gagcggaata ttccgccgca ccacatcttc cagtaacgct
     4501 tcccggatac cataggtcag aagccgggga gtgtcaccgt taaataatcc cggccaccag
     4561 ggcttcaggg tgttcacagc ctcattcagc gtgggcaggt tcagataaat ggacagcacc
     4621 tgcctggcct gcgctttctt taccgccagc tctgcttccc gggccgcttt ctcagccagc
     4681 ttctgctttt tcaccttcca ctttggtggc gtggtgacat tgatgatggt tttcctgctg
     4741 cggacaggcg cttctccttc cgttttccgc ttcagtgtca gtaccggtcg tttttgctct
     4801 gccatgctta ctctgacctt cgtgaaaata tgccctgcag gccgttgaaa caactcatcg
     4861 tagcaggaag ttaacttcaa cgacctttag cgtagtaata ctgtcaatta aatatatttt
     4921 ttaaaccatt gtgtctgcac agctgctaca cgttcaaaaa atgcgccctc ataaatatca
     4981 taatggcatg cgtcagcttc ctcgtataat tccttagtac cagaagcaac agcatcatat
     5041 aatgctcttc cctgttccgg tggattaacg ctatcttggc cagcaataac aaccaaaact
     5101 gggcactgga ctctggcagc actctctgcc ggtttatatt gcaacgtttc catgacagta
     5161 aggaaagg
//
LOCUS       DAPGWD010000226         5136 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPGWD010000226 DAPGWD010000000
VERSION     DAPGWD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.228"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..492
                     /locus_tag="RHX88_004204"
     CDS             <1..492
                     /locus_tag="RHX88_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HDV2232815.1"
                     /translation="DYILDRTMLRPRDAINFVNICLSECDGMVNMNENIVLIAEEKFY
                     SSRKRALVTEWKSIYPFIEEYLDVLSLISFSDFTVSNIGQAVKDDISQHLLDRLTVVK
                     EDDQHTKILMNFEELLKVWFVVGVVGIRKTASLIIYSSFDKPNLDITDMKRDFVIHPL
                     FDR"
     gene            723..878
                     /gene="hokD"
                     /locus_tag="RHX88_004205"
     CDS             723..878
                     /gene="hokD"
                     /locus_tag="RHX88_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDV2232816.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1107..1358
                     /locus_tag="RHX88_004206"
     CDS             1107..1358
                     /locus_tag="RHX88_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232817.1"
                     /translation="MMNIEELRKNFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            1425..1703
                     /locus_tag="RHX88_004207"
     CDS             1425..1703
                     /locus_tag="RHX88_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232818.1"
                     /translation="MARNVKYYNSDNSPVLACTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            1705..2754
                     /locus_tag="RHX88_004208"
     CDS             1705..2754
                     /locus_tag="RHX88_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV2232819.1"
                     /translation="MRVLLRPVLVPELGLVVVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRTAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLPAEKNCSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPSQEKTVVSITVDPESPESFMKLPKRHRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHDELHTGTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            2772..3149
                     /locus_tag="RHX88_004209"
     CDS             2772..3149
                     /locus_tag="RHX88_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV2232820.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVRSRPQ
                     CCDDDAMIICECMARLKKNNSDLHDLLVDYYVGGMTFMALARKHGRSDCWVGRMLQKA
                     EGVVEGMLMVLDLRLEMDADCSK"
     gene            complement(3305..3829)
                     /locus_tag="RHX88_004210"
     CDS             complement(3305..3829)
                     /locus_tag="RHX88_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV2232821.1"
                     /translation="MKLWPVLTGIALSFTLIACKAPTPPKGVQPITNFDANRYLGKWY
                     EIARLENWFERGLEQVSATYEKRNDGGIRVLNRGYDPTKNKWSESEGKAYFTGDTKTA
                     ALKVSFFGPFYGGYNVIKLDDEYKYALVSGPNREYLWILARTQTIPDNVKADYVRTAQ
                     KLGFNVNELLWVKQ"
     gene            4022..4981
                     /locus_tag="RHX88_004211"
     CDS             4022..4981
                     /locus_tag="RHX88_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HDV2232822.1"
                     /translation="MIKKPVIGISGCLGGSAVRFDGGHKRDDFLMDKLVEWVTFRPVC
                     PEMAIGLPVPRPALRLVRSKQGNIRMCFSHDQNEDVTERMTEFSRSYMDKLKDVSGFV
                     VCAKSPSCGMERVRVYDENGNRGRKDGVGLFTSTLMEKFSWLPVEEDGRLHDPVLREN
                     FIERVFALHELNHLYKEKLSRRELLAFHSRYKLQLLAHSQAGYKDMGPFVAAIHEWAD
                     LESYFEVYRDNLMAILRKPASRKNHTNVLMHIQGYFSNYLSTRQRKELSEVILNYRFG
                     TLPLLAPLTLLKHYLGEYPNDYLLTQNYFDPYPEELALRLMVN"
BASE COUNT         1404 a         1031 c         1279 g         1422 t
ORIGIN      
        1 gattatatcc ttgacagaac aatgcttagg cctagagatg caattaattt cgtaaatata
       61 tgtctttctg agtgcgatgg tatggttaac atgaatgaaa atattgtgtt aattgccgaa
      121 gagaaatttt attcgtccag aaagagagct ttggttacag aatggaaaag catatatccg
      181 tttatcgaag aatatttgga tgtgctttcc ttgatttcat tttctgattt tacagtctca
      241 aacataggac aagcagtaaa agatgatata tcgcagcatt tacttgatag attgactgta
      301 gttaaagagg atgatcaaca tacaaaaata ttgatgaatt ttgaggagtt acttaaagtc
      361 tggttcgtcg taggcgtggt tggcataagg aaaacagcat ctttaattat atattcatct
      421 tttgataaac ctaatttgga tattactgat atgaagagag attttgtaat acatccgttg
      481 tttgataggt aatagcaatg tgaatatgtt gagactactg gaaaagctat aactccttta
      541 gtaaagtttc cgtgggtgct tgaggctatc tgtctcaggc atgaacacca aaggcagata
      601 gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggcca
      661 atttcatgct agacacatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg
      721 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag
      781 tgacggcact ggtaacgagg aaagaccttt gcgaggtacg aattcgaacc ggccagacgg
      841 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gacccggcga gggagaaatc
      901 cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc cgcttcggcg
      961 ggtttttgtt tttattttca acgcgtttga agttctggac ggtgccggaa tagaatcaaa
     1021 aatacttaag tagcgcgcag ggagaagagg gatggtccct taaaggggag agctaattat
     1081 ccggaaggat tctgatgatg aacatgatga tgaacatcga agaactgcgt aaaaattttt
     1141 gtgaagatgg cctctatgct gtgtgcgttg aaaatggaaa tcttgttagt cattaccgca
     1201 ttatgtgttt gcgaaagaat ggggctgcgt taattaattt tgtggatggt cgagtgacag
     1261 acggatttat cttgcgcgaa ggtgagtttg tcacttcatt acaggcactg aaagagatcg
     1321 gaataaaagc aggcttttca gcttttgcag aagaataaac tcatctacaa tcttgcgcgg
     1381 ggctgaactc ccgctgagta acaccgtgcc accggagaaa accgatggca cgcaacgtaa
     1441 aatattacaa ttctgataat tcgcccgttc ttgcctgcac gcacgagcgg tattctcacg
     1501 cattcaagtc tgaatggttc cagcaccctc catgcactga agagcaggct gaatggataa
     1561 ttcagtgtta ccgcaggcgc ggatacgagg ttaagaaagc ccttagtctc gactaccgtc
     1621 actggataat ctcagtcaga ttgccttact ccgaacgccc accgcgtccg tcccgtacat
     1681 tccagcaacg catctggagg taacgtgcgg gtattacttc gacctgttct ggtaccggaa
     1741 ctcgggctgg tggtcgttaa gccgggccgt gaatccatgc cggtattcca caatacccgg
     1801 gtactggtgg agccggaacc gaaaagcatg cgtaatctgc cgtccggggt cgttcctgcc
     1861 gttcgccagc cgctggtgga agacaaaaca ttgctgccgt ttttcagtaa tgcacgggta
     1921 attcgtactg ctggtggtgc tggtgcattg tctgactggc tgttgcgcca tattaaatcc
     1981 tgccagtggc cacacggcga ttatcatcac agcgaaaccg ttattcaccg ttatggtacc
     2041 ggcgcaatgg tgttgtgctg gcactgcgac aaccagctgc gtgaccagac atccgaatca
     2101 ctcgagcaac ttgctcatca aaacctgtca gcatggatga ttgacgtcat cggtcacgca
     2161 ataagcggta cgcaggagcg tgaattatct ctggctgaat tatcctggtg ggcggtccgc
     2221 aatcaggtgg cggacgcgct accggaagcg gtattacgtc gctcgctggg attaccagcg
     2281 gaaaaaaact gctcggtgta ccgcgaaagc gacatcgtac cgggagagca gaccgccacc
     2341 agcatattga aacagcgcac aaaaaatctt gcaccgttgc ctcacgccca ccagcaacaa
     2401 aacccatcac aggaaaaaac ggtggtaagc atcaccgttg atcctgagtc tccggaatct
     2461 ttcatgaagc tgcctaaacg tcaccgttgg gttaaggaga aatacacacg ctgggttaag
     2521 acacagccgt gtgcttgctg cggtatgcca gccgacgatc cgcatcatct gattggtcac
     2581 gggcagggcg gaatgggaac caaagcacat gatctctttg tgttgccttt gtgcagaaag
     2641 catcacgacg agttgcatac gggtaccgtg gcatttgaag agaagtatgg ctcccaactg
     2701 gagctgattt ttcgatttat cgatcgcgcg ctggcaattg gtgtgctggc ctaattttgt
     2761 ggagtaagtt gatgcgtgat attcagatgg ttcttgagcg ttggggggca tgggcggcta
     2821 ataatcatga agatgtgacc tggtcgtcca ttgccgccgg ttttaaggga ttaattcctt
     2881 caaaagtaag atctcgtccg caatgctgtg acgatgacgc gatgatcatt tgtgaatgta
     2941 tggctcgcct gaaaaaaaac aacagcgatt tacatgattt attggtggac tattatgtcg
     3001 gcggcatgac ttttatggcg cttgcacgta agcatgggcg atctgattgt tgggttggca
     3061 ggatgctcca gaaagctgag ggcgtagtgg agggcatgct gatggtgttg gatctccgat
     3121 tggagatgga tgctgattgt tcgaaataat caaaggaaaa gttgctgtct gattttcatt
     3181 agtctaacat tttaaatgtt ggaatcgcaa cgtagttatt atcatataac agcttgtttc
     3241 ctgatttagc cagcctcccc aaaggctggt ttttttctaa taagtatcat ttcgggtggg
     3301 gattttattg tttaacccat aatagttcat tgacattgaa tcccaacttt tgagcggtac
     3361 gcacatagtc tgcttttaca ttatctggaa tagtttgggt ccttgccaga atccataggt
     3421 attctctgtt cggaccactg acaagagcat acttatactc atcatccagc ttgattacat
     3481 tatagccacc atagaagggg ccaaaaaacg aaactttcaa cgctgcagtt ttagtatctc
     3541 cagtaaagta tgctttacct tcgctctcgc tccatttgtt tttcgttgga tcgtatccac
     3601 ggttaagtac gcgaatccct ccgtcgttcc gtttttcata agtagcgctg acttgttcca
     3661 gaccacgttc gaaccagttc tcgaggcgag ctatttcata ccattttccg aggtagcggt
     3721 tggcgtcaaa atttgtaatc ggctgcacac ctttaggtgg tgtcggggcc ttacatgcta
     3781 taagagtgaa agagagtgca atgccagtca acacaggcca taacttcata ataaatcctg
     3841 tacttttgat agttgagagt aagtatgaaa gatagatgag tacgaccgat cacttaaaga
     3901 actttcctac tatattagga atagtccata acagaaaaat tgtcagtgat gaagccagaa
     3961 aggcaattta gtccgtgcat tacaaagttt atgtgttaat gaattagtca agggggagga
     4021 aatgataaaa aaacctgtga ttgggatcag cggttgtttg ggcggttctg ctgttcgttt
     4081 tgatggtggt cacaaaagag atgacttttt aatggacaaa ttagtggaat gggtaacatt
     4141 cagaccagta tgtccggaaa tggctatagg gctgccagtt ccgcgtcctg ctctacgact
     4201 tgtgcgctcg aagcaaggaa atatacggat gtgtttcagc cacgaccaga atgaggatgt
     4261 gacagagaga atgacagagt ttagtcgttc ttatatggac aaattaaagg atgtatcggg
     4321 gtttgtggtt tgtgctaaat ctcccagctg tggcatggag cgcgtgcgtg tctatgatga
     4381 aaatggtaat cgaggtcgta aagatggagt gggactattt acgagcactt tgatggaaaa
     4441 gttttcctgg ctaccggttg aagaggatgg gcgattacat gatccagtgc ttcgtgagaa
     4501 ttttattgaa agagtttttg ctttgcatga gctcaatcat ctttacaagg agaaattatc
     4561 aagaagagag ttattagctt ttcatagtcg ttataagctt cagttgttgg cgcatagcca
     4621 ggcaggctat aaagatatgg gaccatttgt ggctgcaata cacgagtggg cggaccttga
     4681 atcatacttt gaggtgtatc gtgataatct gatggcgatt ctcagaaaac cagcatcacg
     4741 taaaaatcac acgaatgtgc tgatgcatat acaggggtat tttagtaatt acttaagtac
     4801 acgccagcgt aaagagttga gcgaggttat acttaactat cgctttggca cattgcctct
     4861 tcttgcgccg ttgactctgc tgaagcatta tctgggtgag tatcctaatg attacttgct
     4921 tacacagaat tacttcgatc cctatccgga agaactggct ctaagactga tggtgaatta
     4981 actgtatgcg atatcatctg aaagtgatga gttcctgtat gcaggatatt tacaatcgta
     5041 aaaactacgc tatgatgccc atagtgtcag tttgtataaa aatcctgttc acgctaaaga
     5101 aaacattgag atgcaattta atgttggtaa acatac
//
LOCUS       DAPGWD010000227         5009 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPGWD010000227 DAPGWD010000000
VERSION     DAPGWD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.229"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1200
                     /gene="tssI"
                     /locus_tag="RHX88_004212"
     CDS             <1..1200
                     /gene="tssI"
                     /locus_tag="RHX88_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV2232823.1"
                     /translation="LTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGTALENHFAV
                     IPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNPADQDSS
                     CWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGT
                     KTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETI
                     GNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGG
                     IMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHL
                     ELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQD
                     TPDMREY"
     gene            1267..>5009
                     /gene="rhsD"
                     /locus_tag="RHX88_004213"
     CDS             1267..>5009
                     /gene="rhsD"
                     /locus_tag="RHX88_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HDV2232824.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLAGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGLWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQS
                     QHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEP
                     EYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPG
                     QQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPL"
BASE COUNT         1139 a         1394 c         1677 g          799 t
ORIGIN      
        1 ctgacggggc atccgcaggc gaacctgaac cgggaatggc aggtggtggc aagtgaactg
       61 cacggcgaac agccacaggc ggtgccagga cggcagggag cggggacggc gctggagaac
      121 cattttgcgg tgatcccggc agacagaaca tggcgaccac agccgttgct gaaaccgctg
      181 gtcgacggcc cgcagagcgc tgtcgtgaca ggaccggcag gcgaggaaat cttctgcgac
      241 gaacatggtc gcgtgcgggt gaagttcaac tgggaccgtt ataacccggc agaccaagac
      301 agttcgtgct ggatccgtgt ggcacaggcg tgggcaggca ccggttttgg ccacctggcg
      361 ataccgcgtg tgggtcagga ggtgattgtg gacttcctca acggcgatcc ggaccagccg
      421 attattatgg ggcgtaccta ccaccaggaa aaccgcacac ccggcagcct gccggggacg
      481 aagacgcaga tgaccattcg ttcgaaaacc tataagggca gcgggtttaa tgaactgaag
      541 tttgacgatg cgacagggaa agaacaggtc tacatccacg cgcagaagaa catgaacacc
      601 gaggtgctga ataaccgcac cacggatgtg ataaacaacc atgctgagac cattggcaac
      661 aatcagatga ttgcggttac caacaatcag atacagacgg tgggcgttaa ccagatagag
      721 acggtgggca gtaaccagat cattaacgtg ggttctgttc aggtggaaac gattggactt
      781 gttcgtgcgc tgaccgtggg cgtggcgtac cagacgacgg taggtggcat tatgaacact
      841 tcggtggctc tgatgcagtc ctcgcagatc ggtttgcata aatcgttgag ggtcgggctg
      901 ggttatgacg tcaaagtcgg aaataacgtt accttcaccg ttggtaaaac gaaaaaggat
      961 gataccgggc agaccgcgat ttactccgcc ggtgagcatc tggagctctg ctgtggtaag
     1021 gcaaggctgg tgctgacgaa ggacggacaa atttttctca acggcacaaa aattcatttg
     1081 cagggtaagg agcaggttaa tggtgactca ctgttgatta actggaactg tgcagcctcg
     1141 aaatccccac cgaagacccc tgatgaaaag caggatacgc cggatatgag agagtactga
     1201 tcccgtacct gttcggtatt cagtaacaaa caagcattaa aaataaacag aagaaggaat
     1261 tgaatcatga gcggaaaacc agcggcgcgt cagggagata tgactcagta tggcggtcct
     1321 attgtccagg gttcggcagg tgtaagaatt ggcgcgccca ccggcgtggc gtgctcggtg
     1381 tgtccgggcg ggatgacttc gggcaacccg gtaaatccgc tgctgggggc gaaggtgctg
     1441 cccggcgaga cggaccttgc gctgcccggc ccgctgccgt tcattctctc ccgcacctac
     1501 agcagctacc ggaccaggac acccgcgccg gtgggagtgt ttggccccgg ctggaaagcg
     1561 ccttctgata tccgcttaca gctacgcgat gatgcactgg tactcaacga caacggcggg
     1621 cggagcattc actttgagcc gctgctggcg ggggaggcgg tgtacagccg cagcgagtca
     1681 atgtggctgg tgcgcggtgg taaggcagcg cagccggacg gccacacgct ggcgcggctg
     1741 tggggggcgc tgccgccgga tatccggtta agcccgcatc tttacctggc gaccaacagc
     1801 gcacaggggc tgtggtggat actggggtgg tcagagcggg tgccgggtgc tgaggacgta
     1861 ctgccagcgc cgctgccgcc gtaccgggtg cttaccgggc tggcagaccg tttcgggcgg
     1921 acgctgacgt accggcgtga ggccgcaggc gacctggccg gggaaatcac cggcgtgacg
     1981 gacggtgccg ggcgtgagtt ccgtctggta ctgaccacac aggcacagcg ggcggaagag
     2041 gcccgcacct cttcgctatc ttcttctgac agttcccgcc ctctctcagc ctcaccgttc
     2101 cccgacacac tgcccggtac cgaatacggc cccgacaggg gcatccgtct ttcggcggtg
     2161 tggctgatgc acgacccggc atacccggaa agcctgcccg gtgcgccact ggcgcggtac
     2221 acgtatacgg aagccggtga actgctggcg gtatatgacc gcagcaatac gcaggtgcgc
     2281 gctttcacgt atgacgcgca gcacccgggc cggatggtgg cgcaccgtta cgcgggaagg
     2341 ccggagatgc gctaccgcta cgacgatacc gggcgggtgg tggagcagct gaacccggca
     2401 ggcctgagtt accgctacca gtatgagcag gaccgcatca ccgtcacgga cagcctgaac
     2461 cggcgtgagg tgctgcatac agaaggcggg gccgggctga agcgggtggt gaaaaaagaa
     2521 ctggcggacg gcagcgtcac gcacagcggg tatgacgcgg caggaaggct cacggcgcag
     2581 acggacgcgg cgggacggcg gacagagtac ggtctgaatg tggtgtccgg cgatatcacg
     2641 gacatcacca caccggacgg gcgggagacg aaattttact ataacgacgg gaaccagctg
     2701 acggcggtgg tgtccccgga cgggctggag agccgccggg aatatgatga accgggcagg
     2761 ctggtatcgg agacatcgcg cagcggggag acagtacgct accgctacga tgacgcgcac
     2821 agtgagttac cggcgacgac aacggatgcg acgggcagca cccggcagat gacgtggagc
     2881 cgctacgggc agttgctggc gttcactgac tgctcgggct accagacccg ctatgaatac
     2941 gaccgcttcg gccagatgac ggcggttcac cgcgaggaag gtatcagcct ttaccgccac
     3001 tatgacaacc gtggccggtt aacctcggtg aaagacgcac agggccgtga aacgcagtat
     3061 gagtacaacg ccgcaggcga cctgactgcc gttatcaccc cggacggcaa ccggagcgag
     3121 acacagtacg atgcgtgggg aaaggcggtc agcaccacgc agggcgggct gacgcgcagt
     3181 atggagtacg atgctgccgg acgtgtcatc agtctgacta acgagaacgg cagccacagc
     3241 gacttcagct acgatgcgct ggaccggttg gtacagcagg gcggctttga cgggcggacg
     3301 caacgttatc attatgacct gaccggaaaa ctcacgcaga gtgaagatga ggggcttgtc
     3361 accctctggt actacgatga atcggaccgt atcactcacc gcacggtgaa cggcgaaccg
     3421 gcagagcagt ggcagtatga tgaccacggc tggctgacag acatcagcca cctgagcgaa
     3481 ggccaccgtg tcgctgtcca ctacggctac gacgataagg gccgcctgac cggcgaacgg
     3541 cagacggtgg agaacccgga gacgggggaa ctgctgtggc agcatgagac aacacacgcc
     3601 tacaacgaac aggggctggc aaaccgcgtc acgccggaca gcctgccgcc ggtggagtgg
     3661 ctgacctacg gcagcggtta tctggcgggc atgaagcttg gcgacacgcc gctgctggag
     3721 tacacgcgcg accgcatgca ccgtgagacg gtgcgcagct ttggcagcat ggcaggcagt
     3781 aatgccgcat ataaactgac cagcacatac acccccgcag gccagttaca gagccagcac
     3841 ctgaacagcc tggtgtatga ccgtgactac gggtggaatg acaacggcga cctggtgcgc
     3901 atcagcggcc cgcgacagac gcgggaatac ggctacagcg ccacgggcag gctggagagc
     3961 gtgcgtaccc tcgcaccgga cctggacatc cgcatcccgt atgccaccga cccggcggga
     4021 aaccgcctgc cggacccgga gctgcacccg gacagtaccc tcacggcgtg gccggataac
     4081 cgcatcgcag aggatgcgca ctatgtttac cactatgatg aatacggcag actgacggag
     4141 aagacggacc ttatcccggc gggtgttatc cggacggacg acgagcggac ccaccactac
     4201 cactacgaca gccagcaccg tctggtgttc tacacgcgga tacagcatgg cgagccactg
     4261 gtcgagagcc gctacctcta cgacccgctg ggacggcgaa tggcgaaacg ggtatggcgg
     4321 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggagat gacgtggtac
     4381 gggtgggacg gcgaccggct gacgacggta cagaccgaca ccacgcgtat ccagacggta
     4441 tatcagccgg ggagcttcgc gccgctcatc cgcatcgaaa cagacaacgg cgagcgggag
     4501 aaagcgcagc gccgcagcct ggcggagaag ctgcagcagg aagggagcga ggacgggcac
     4561 ggcgtggtat ttccggctga actggtgcgg ctgctggaca ggctggagga agaaatccgg
     4621 gcagaccgcg tgagcagtga aagccgggcg tggcttgcgc agtgcgggct gacggtggag
     4681 caactggcca gacaggtgga gccggaatac acaccggcgc gaaaagttca tttttaccac
     4741 tgcgaccacc ggggcctgcc gctggcgctc atcagcgaag acggcaatac ggcgtggcgc
     4801 ggggagtatg atgaatgggg caaccagctt aatgaagaga acccgtatta cctgcaccag
     4861 ccataccgtc tgccggggca gcagcatgat gaggaatcag ggctgtacta taaccggaac
     4921 cggtactatg acccgctaca ggggaggtat attacacaag accccattgg gctggcgggg
     4981 ggatggaacc tgtataatta cccactgaa
//
LOCUS       DAPGWD010000228         4983 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPGWD010000228 DAPGWD010000000
VERSION     DAPGWD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.230"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(370..746)
                     /gene="rnpB"
                     /locus_tag="RHX88_004214"
     ncRNA           complement(370..746)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RHX88_004214"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(779..1924)
                     /gene="garK"
                     /locus_tag="RHX88_004215"
     CDS             complement(779..1924)
                     /gene="garK"
                     /locus_tag="RHX88_004215"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDV2232825.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2021..2911)
                     /gene="garR"
                     /locus_tag="RHX88_004216"
     CDS             complement(2021..2911)
                     /gene="garR"
                     /locus_tag="RHX88_004216"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV2232826.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2941..3711)
                     /gene="garL"
                     /locus_tag="RHX88_004217"
     CDS             complement(2941..3711)
                     /gene="garL"
                     /locus_tag="RHX88_004217"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDV2232827.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3727..>4983)
                     /gene="garP"
                     /locus_tag="RHX88_004218"
     CDS             complement(3727..>4983)
                     /gene="garP"
                     /locus_tag="RHX88_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDV2232828.1"
                     /translation="TAVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYLLMQ
                     IPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSEAP
                     SFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTVMGV
                     IGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHYIK
                     QLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAG
                     GVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFG
                     KGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVF
                     VGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1187 a         1449 c         1198 g         1149 t
ORIGIN      
        1 ttattaatac cgtagatatt tattcatctt accctgtgca ccaaaagcaa aaactcactg
       61 acgcgggttt gaaactgatg gcaaccgcaa aaatgcctga tgcgctacgc ttatcaggcc
      121 tacgccatct ctgcaatata ttgaatttgc gtgcttttgt aggcaggata aggcgttcac
      181 gccgcatccg gcatgaataa agcgcactca acaatctgaa acccgccgga acggtttatt
      241 acgtacatca ggtgaaactg accgataagc cgctttcttt tgggtatagc gtcgtggaca
      301 gtcattcatc tttctgcccc tccaaaagca aaaacccgcc ggtttacgct ggcgggtttt
      361 cgttaaatca ggtgaaactg accgataagc cgggttctgt cgtggacagt cattcatcta
      421 ggccagcaat cgctcactgg ctcaagcagc ctacccgggt tcagtacggg ccgtacctta
      481 tgaaccccta tttggccttg ctccgggtgg agtttaccgt gccacggact gttaccagcc
      541 gcgcggtgcg ctcttaccgc accctttcac ccttacctga tcccgcttgc gcgggccatc
      601 ggcggtttgc tctctgttgc actggtcgtg ggtttccccc ccaggcgtta cctggcaccc
      661 tgccctatgg agcccggact ttcctcccct ccgcccgtct cccccgaaga ggacgacgac
      721 gaagcggcga ctgtctggtc agcttcggcg cgcagtatag agggtttgcg cgcccttgtc
      781 accccgcgtt gcgcattcca atcgccagtg tcgcggcgat attacgtgaa gcacggcaga
      841 tattgtcata agccccgcgg aatgcttcgt ccaacgtacc tatgctggtc aatacgctga
      901 agaccgcatc aatgccatgc tgatgtacaa cgccaacatc atcggtcagg ctacccgcaa
      961 tgccaatcac cggtttatgg tacttcttcg ccacgtttgc gacaccaatc ggtaccttcc
     1021 cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta caatcgtgaa
     1081 tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc agttccgcac
     1141 caagaaacgc cattagcgcc gcgcccatac cacccgcagc tcctgcaccg gggacatctt
     1201 tcacatcaac atgcagcgct tttttaatga cctcggcata gtgagagagg ttattgtcca
     1261 gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt
     1321 tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc
     1381 gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac
     1441 caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc
     1501 cgccatcatt tgtagcgctg ccgccaatgc cgataataat gtttgtcgca ccgctctcca
     1561 gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt
     1621 ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg gttttgccat
     1681 cgccggagat cccccaactg gcattcactt tctcgcccag cggccctgta acccaggcgt
     1741 gacgttcagc cccctgggtg gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg
     1801 caaccggaac agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg
     1861 cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt
     1921 tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc ggcctggatt
     1981 ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta acttcgactt
     2041 tcgccagttt ttcgtagtag cacgccaggg cgctatgatc cgccgttcct aaaccatctg
     2101 ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgt gcgccgacgc
     2161 cgtgagaagt atccagcgca ttcgccagat ccttaatatg cagatcaata cggaagcccg
     2221 gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca
     2281 gtccaccgcg aattgcctga taaaccaggt ccgggttaac gcccgcttta gttgccagcg
     2341 ttaacgcttc tgacatcgcg gcaatattca gcgccacaat gacctgattt gccagtttgg
     2401 tgacgttacc tgcaccgatt tccccggtat gcaccacgga acccgccatc gctttcatca
     2461 aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt
     2521 cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt
     2581 tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata
     2641 ccgtacctgg cttcgcgcct tcaatgatgc cattctcacc cagcgccacc tctttcacat
     2701 gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc gctttagccg
     2761 tagacgctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg ttacggtcag
     2821 caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta cccataatcc
     2881 ccaggccaat aaaacctact ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg
     2941 ttatttttta aaggtatcag ccagtttctg agtggcagag cggaagacgc caagatcgct
     3001 gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg cttcgaccgg
     3061 cgcgagaata ccgctgggtt tgccgtgcgc actggcacgg ttaaaaatgt gctgaattgc
     3121 tttttgtaca tccgggtgcg aggcattgcc gagatggcct aatgccgcag ccagatcgct
     3181 ggggccgacg aagataccgt ctacaccttc ggtagcggca atggcatcga cgttatctac
     3241 gccctgctga ctttctatct ggaccagaat ggtgatgttc ttgttcgact gagcgaaata
     3301 atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc
     3361 cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg
     3421 aatcaggaag ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt
     3481 cggcactcgc actactggcg cgctggcgct gcctttcagg gccattaact gcggaataaa
     3541 cgtggagata tcgtttggcg catgttcgcc atccagcacc agccagtcaa acccagccaa
     3601 accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa tttgtacctg
     3661 tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac
     3721 ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac
     3781 catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc
     3841 acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt
     3901 aaagacgccg ccacagaggc caacaatctc tttcggcgcg gtgtcagaaa tcaccggcca
     3961 gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt
     4021 ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat
     4081 cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat ccgagaagac
     4141 acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga ccagacccac
     4201 tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg tgaggaagaa
     4261 ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca tgcggttaga
     4321 gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc ccggcttttt
     4381 gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt ccgcagacat
     4441 acgtgggtga tctgtcgggt tatgaatcaa tttgatccac agcgccgtca gcacaaaacc
     4501 aatcaccccc ataacggtaa agacgtgctc ccagccccag gcgaaagtca gccagccaag
     4561 cagaggcgaa aagagcgcca gcgagaaata ttgcgccgag ttaaagatgg cggaggcagt
     4621 accacgttct ttcgtcggga accaggcggc gacaatacgg gcgttcgccg ggaatgatgg
     4681 cgcttccgag aagccgagca taaagcgcat aaagaacatg gagatccctg cccaggccag
     4741 cgggaacata tcaacaaagc cttgcaggaa agtgaatagc gaccagaaaa agaggctata
     4801 ggtataaact tttttcgagc caaacttatc aagcagccag ccgccgggga tttgcatcag
     4861 caagtaggcc cagccaaaag cagaaaagat gtaacccatc gaaaccgcac ttaactgcaa
     4921 ctcttttgcc acttcggtac cagcaataga cagcgttgca cgatcggcgt agttaacggc
     4981 ggt
//
LOCUS       DAPGWD010000229         4861 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPGWD010000229 DAPGWD010000000
VERSION     DAPGWD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.231"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            152..1126
                     /gene="hemB"
                     /locus_tag="RHX88_004219"
     CDS             152..1126
                     /gene="hemB"
                     /locus_tag="RHX88_004219"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDV2232829.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1232..2083)
                     /gene="tauD"
                     /locus_tag="RHX88_004220"
     CDS             complement(1232..2083)
                     /gene="tauD"
                     /locus_tag="RHX88_004220"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDV2232830.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLNGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2080..2907)
                     /gene="tauC"
                     /locus_tag="RHX88_004221"
     CDS             complement(2080..2907)
                     /gene="tauC"
                     /locus_tag="RHX88_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDV2232831.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2904..3671)
                     /gene="tauB"
                     /locus_tag="RHX88_004222"
     CDS             complement(2904..3671)
                     /gene="tauB"
                     /locus_tag="RHX88_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDV2232832.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3684..4646)
                     /gene="tauA"
                     /locus_tag="RHX88_004223"
     CDS             complement(3684..4646)
                     /gene="tauA"
                     /locus_tag="RHX88_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2232833.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1118 a         1403 c         1242 g         1098 t
ORIGIN      
        1 gtgaatatga aattattgct atttctatta caaagatgtc ctggtctctc tttcattctc
       61 ttcttgtcaa catcacgaca actttcgtaa aacactcctg ccccgcttca ggtatactgt
      121 gcaccttaat tccacaaaca tcaggcagac catgacagac ttaatccaac gccctcgtcg
      181 cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag acaacactta gccttaacga
      241 cctggtgttg ccgatctttg ttgaagaaga aattgacgac tacaaagccg ttgaagccat
      301 gccaggcgtg atgcgcattc cagagaaaca tctggcacgc gaaattgaac gcatcgccaa
      361 cgccggtatc cgttccgtaa tgacttttgg catctctcac cataccgatg aaaccggcag
      421 cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg cgcatctgca agcagactgt
      481 gccagaaatg atcgtcatgt cagacacttg tttctgtgaa tacacttctc acggtcactg
      541 cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact ctggaaaatt taggcaagca
      601 agccgtggtt gcagctgctg caggcgcaga cttcatcgcc ccttccgccg cgatggacgg
      661 ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc tttaaagata cggcgattat
      721 gtcgtattcg accaagttcg cctcttcctt ttatggtccg ttccgtgaag ctgccggaag
      781 cgcattaaaa ggcgaccgca aaagctatca gatgaaccca atgaaccgtc gcgaggcgat
      841 tcgtgaatca ctgctggatg aagcccaggg cgcagactgc ctgatggtta aacctgctgg
      901 agcatacctc gacatcgtgc gtgagctgcg tgaacgtact gaattgccga ttggcgcgta
      961 tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg ctggcgggtg ctatagatga
     1021 agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt gcgggtgcgg atctgatttt
     1081 cagctacttt gcgctggatt tggctgagaa gaagattctg cgttaatttt tttcctctca
     1141 gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc cctcacccta accctctccc
     1201 caagggggcg agggaaccgt ccgcactcgt atcaccccgc ccgataaaac ggtttatccc
     1261 caagaatcgt cgcccgatgc ataatccgcc gctgtggcag gtaatcggca ttggcatagt
     1321 gctgagtcac gcggttatcc caaatcgcaa tatcattcgg ttgccagcgc caacgcacct
     1381 gaaactccgg tttagtgata tgggcgaata aaaagcctaa caaggcttcg ctctctttct
     1441 cgctgacatc gacaattcgc gtagtaaagc cttcattcac aaacagcgcc tgtttaccgc
     1501 tcaccggatg cgttcgcacc accggatgta gcaacggagg atttttcgcg accgcctcgc
     1561 gccagcgttg atgttcctcc tcggttttgc ggtatttgta ttccgggaac gatttacgga
     1621 aatcatgctc cgcacgcagc ccgttcagca gctggcggaa gggaacagag agcgcctcat
     1681 aggccgcaat accgctggtc cagagcgtat caccgccggt cgaaggtaac tctttagctg
     1741 ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac atcggtatgc cagttgtcgt
     1801 tatccggcgg attatcgtta tgggtatcca gcacgatgat ctcgtcaacc ccttcggcat
     1861 gagggtaaac agggtgaata tgcagttcgc caaaacgctg ggccagcgcg cgttgctgct
     1921 gcggcgtaat agtttgatcg cgcagaaaca ccacctgatg gcgcagcacc gcatggtaaa
     1981 gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc ggcacccgaa atttgtgcgc
     2041 cgatatacgg ccccagcggg gtaatgctca gacgttcact cattgtactt ctccatgcca
     2101 gggcgtcaga cggcgctgta acgcgcgcaa acccagttct aaaagaaagg cgataatcgc
     2161 aatcaccgcg atccccgcca gcaccacgtc agtcgcgaga aattcacccg ctgactgaac
     2221 cataaatcct aaaccgcgcg tcgcggcaat cagctccgcc gctaccagcg tagaccagcc
     2281 cacacccaga ccaatgcgta atccggtgag gatttccggc agcgcgccgg gcaaaatgac
     2341 aaaccacagc acctgcgcac ggctggcacc cagcgactgg gcggcacgaa tgcgaacctg
     2401 ctgcgcgctt ttcacccccg ccagcgccga catcgccacc ggtgcaaaaa tcgctaaata
     2461 gatcagtaag atcttcgagg tttcaccaat accaaaccag atcaccatca gcggcaaata
     2521 agccagcggc ggcaccggac gataaagttc gattaccgga tccagaatgc cgcgcaccgt
     2581 cgggcttagc cccatggcga tcccgaccgg aataccgatc accaccgccg cgagtagcgc
     2641 tagcacaatg cgcgtcaggc tggctgccag atgctgccac agcgtggcgt ccataaatcc
     2701 ctgcggtccg gcaatggtga gcagttttgc cagtacttgt tgcggcggcg gcaaaaacag
     2761 cgggctaatc agttgcagcg ccgccaccgt ccaccataca gtgagtaaaa ccgctaacgt
     2821 gccaatgctt aaggtcacct gacgcgagag cggccagcgc catttcagcc gccgcgaatg
     2881 cagtttttca ttaatgagca cactcatgag aacgcctccc gttgctcaaa tacgcggctt
     2941 aaaacatatt cgcgcatggc gatgaattgt ggatcggact tgatgctgcg gctcgactct
     3001 cccgcaacaa agcggcgagc aaagttgagc ggcagccgct ccagcacacg gccagggccg
     3061 gatgaaagca gaaccagttc agtcgccata aacaccgctt cttctatatc gtgggtaatc
     3121 aacagcacct gcttgcccgt ctcctgccag agtttcagca gcagagtttg catctggtcg
     3181 cgggtgaagg cgtccagcgc accaaacggt tcgtcgagta ataacagctg gggattcgcc
     3241 gccagcgcgc gggcaatccc cacccgctga cgttgaccac cggaaagctg ccagatatag
     3301 cgtttttctg cgccttccag ccccactttt ttcagcatct ggtgcgcgat ttccagtcgc
     3361 tgcattttct ctatacctgc caattgcagg ccgaacgcca cgttgtcctg tacattgcgc
     3421 cacggtagta gcccttcatt ctgaaaaacc accccgcgct ctgctcccgg tccctcaata
     3481 cgcttacccg ccagttgaat gctgccatgc tgataaggca caaaaccggc aatcagattc
     3541 agcagggtgg ttttaccgca gccggacggc cccagcacca ccagtagctc gccgctttcc
     3601 agcgtcaggt tgatatcttc cagtgccggt ttgccgccat aatcggcata gagatgagag
     3661 atttgcagca tctgcgcctc cttttattgc acgaagcgcg aggtaacgta ctggctgtaa
     3721 tcattcgcta cagccgggac cttgccctgc tctttcaaaa actgcgcggt gtcgatgatt
     3781 gctttgttca ccggtccggt cagttctgcc gtttgttgct gcggcgtcag ataggtattc
     3841 cccttcacca gccccggaac gtcaccttca ggcacgccgc ttaaacgcgc cagtttgctg
     3901 atgttttccg gctgtttcag ccacacgtct gggttagcaa tgtacggttg ctgagcatcg
     3961 atggcgcttt tagcgaacgc tttcacgacc tcaggatgtt tctcggcaaa atctttgcgc
     4021 accacccaga cgtccagcgt tggcgcgccc cactgcccga cctgttcaga atcggtcaac
     4081 accttgccgt ctttttccag ggcgttaacc gccggtgccc agacataagc accatcaata
     4141 tctccccgct gccaggcagc gataatcgcg ggcggctgca ggttcacaat ctccacttgc
     4201 ccgggtttaa tgccccagtg tttcagcgcc gccagcaggc tgtagtgggt ggtggagata
     4261 aacggtacgg cgatgcgttt gccaatcaga tcttccggtt tgctgatagt tttctttacc
     4321 accagcgctt cggagttacc cagttttgac gccagcaaga agacttcaat cggcacctgt
     4381 tggctggctg caaccgctaa cgggctggaa ccgaggttgc cgatttgcac atcgcctgaa
     4441 gccagcgccc gcacgatgct ggctccgctg tcaaacttac gccagtccac gctcgccccg
     4501 ctctctttag caaaggtgtt gtcggcctga gccactttcg ccggttcggc tgaggtttga
     4561 tacgccacgg tgacgttcac cgcctgtgcc tgaaaagcga tgaatgccag tgcggctaga
     4621 agtgtgttac gcgatgaaat tgccatgatt gtctgctccc ctgtcttgtt atgggagcag
     4681 tattcaggaa taaaaacatt cattaaaaga attagtcgtt atcgcacaga tgattttatt
     4741 cttagcaaaa aaacggctaa ggaaagatta aatatgcaat tactccgttc taacaattgg
     4801 gaaaattact acacttcatt ttagtgatgc ataaacaacc acattacgag atttattact
     4861 t
//
LOCUS       DAPGWD010000230         4801 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPGWD010000230 DAPGWD010000000
VERSION     DAPGWD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.232"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            201..1358
                     /gene="acrE"
                     /locus_tag="RHX88_004224"
     CDS             201..1358
                     /gene="acrE"
                     /locus_tag="RHX88_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDV2232834.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1370..4474
                     /gene="acrF"
                     /locus_tag="RHX88_004225"
     CDS             1370..4474
                     /gene="acrF"
                     /locus_tag="RHX88_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDV2232835.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVFDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4727..>4801
                     /locus_tag="RHX88_004226"
     CDS             4727..>4801
                     /locus_tag="RHX88_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2232836.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVP"
BASE COUNT         1171 a         1163 c         1240 g         1227 t
ORIGIN      
        1 ctttcaaccc gcgtcaaaat aaaacagtag aatattaatc tttttttgtg tttatgtgcc
       61 ttgagacgcc agtattcata actattcctt acatcgacga atgataattt gtaggatagc
      121 aaactgtatt tttctttctg cgagttaacg cgttgccttt ctgggtaaat aacgcgcttt
      181 tggttttttg aggaatagta atgacgaaac atgccaggtt tttcctcctg ccctccttta
      241 ttctgatctc cgcggcttta atcgccggtt gtaacgataa gggagaagag aaagctcacg
      301 tcggtgaacc gcaggttacc gttcatattg tgaaaacggc tccgttagaa gttaagactg
      361 aattaccagg ccgcaccaat gcttaccgta tagccgaagt tcgcccacag gttagcggga
      421 tcgtactgaa tcgtaatttc actgaaggca gcgatgtaca agcaggccag tccctgtacc
      481 agatcgatcc cgcgacctat caggcaaatt atgacagcgc gaaaggcgaa ctggcgaaaa
      541 gtgaagccgc cgccgccatc gcgcatttga cggtaaaacg ttacgttccg ctcgtgggta
      601 cgaaatacat cagccagcag gagtacgacc aggccattgc tgatgctcgc caggccgatg
      661 ccgccgtgat tgccgcaaaa gccacagtcg aaagcgctcg catcaatctc gcttatacca
      721 aagtgactgc gccaattagc ggacgtatcg gcaaatcgac cgtgaccgaa ggcgctcttg
      781 tcactaatgg gcaaacgact gaactggcga ctgtccagca gctcgatcct atctacgttg
      841 atgtgaccca atccagcaac gattttatga ggctgaagca atccgtagag caaggaaatt
      901 tgcataagga aaacgccacc agcaacgtag agttggtcat ggaaaacggt caaacctatc
      961 ccctgaaagg tacgctgcaa ttctctgatg tgaccgttga tgaaagcacc ggctccataa
     1021 ccctacgtgc tgtcttccct aacccgcaac atacgctttt gccgggtatg tttgtgcgtg
     1081 cacggattga tgaaggcgtc caacctgacg ccattcttat cccgcaacaa ggcgttagcc
     1141 gcacaccgcg tggtgatgca accgtgctga ttgttaacga taaaagtcag gtggaagcgc
     1201 gccctgtcgt tgccagtcag gcgattggcg ataaatggtt gattagcgaa ggtctgaaat
     1261 ctggcgatca agtcattgtc agcggcctgc aaaaagcgcg tccgggagag caggttaaag
     1321 ccactaccga tacccccgca gatactgcat cgaagtaagg taatctgaca tggcaaactt
     1381 ttttattcga cgaccgatat ttgcatgggt gctggccatt attctgatga tggcgggcgc
     1441 actggcgatc ctacaattgc ccgtcgctca gtatccaacc attgcaccgc ctgcggtttc
     1501 tgtttcggca aactatccag gtgctgatgc gcagaccgtg caggatacgg tgacgcaggt
     1561 tatcgaacag aatatgaatg gtatcgataa cctgatgtat atgtcctcca ccagcgattc
     1621 cgccggtagc gtgacaatta cccttacctt ccagtccggg accgatcctg atatcgcgca
     1681 agtgcaggtg cagaacaaac tccagctcgc cacgccgttg ctgccgcagg aggttcagca
     1741 gcaggggatc agtgttgaaa agtccagtag cagctatttg atggtggcgg gctttgtctc
     1801 tgataaccca gacaccacac aggacgatat ctcggactat gtggcctcta acgttaaaga
     1861 tacgcttagc cgtctgaatg gcgtcggtga cgtacagctt ttcggcgcac agtatgcgat
     1921 gcgtatctgg ctggacgccg atctgctaaa caaatataaa ctgacaccgg ttgatgtgat
     1981 taaccagttg aaggtacaga acgatcagat cgctgccgga cagttgggcg gaacgccagc
     2041 gttaccaggg caacagttga atgcctcgat tattgctcaa acgcggttaa aaaatccgga
     2101 agaattcggc aaagtgaccc tgcgcgtaaa cagtgacggc tcggtggtac gcctgaaaga
     2161 tgtcgcacgg gttgaacttg gcggtgaaaa ctataacgtt atcgctcgta tcaacggaaa
     2221 accggcggcg ggcctgggga ttaagctggc aaccggcgcg aatgctctcg ataccgcgaa
     2281 agccattaag gcaaaactgg cggaattaca gccattcttc ccgcagggaa tgaaggttct
     2341 ctacccttat gacaccacgc cattcgtcca gctttctatt cacgaagtgg taaaaacgct
     2401 gttcgaagcc attatgctgg tgttcctggt gatgtatctg ttcttgcaga atatgcgagc
     2461 aacgctgatc cccaccattg cggtacccgt ggtgttatta gggacgtttg ccatcctcgc
     2521 cgcttttggt tactccatca acacactaac gatgttcggg atggtgcttg ccatcgggct
     2581 gctcgtcgat gatgcgatag tggtggtgga gaacgtcgag cgcgtgatga tggaggataa
     2641 gctcccgcca aaagaagcga cggaaaaatc gatgtcgcaa attcagggcg cactggtggg
     2701 tatcgcgatg gtgctgtcag cggtatttat tccgatggca ttcttcggcg gttctactgg
     2761 ggcaatttat cgccagttct ctatcaccat cgtttcggca atggcgcttt ctgttctggt
     2821 ggcattgatt cttacccctg cgttatgtgc aacactgctc aaacccacct cagctgagca
     2881 tcacgaaaat aagggtggtt tcttcggctg gtttaatacc accttcgatc atagcgttaa
     2941 ccactacacc aacagcgtcg gcaaaatcct cggttccaca ggacgatatt tactgatcta
     3001 tgcgctgatt gttgcaggaa tggtggtgtt gtttttacgt cttccgtctt ccttcttacc
     3061 tgaagaggat cagggtgtct ttctgaccat gattcagtta cccgctggcg cgacgcaaga
     3121 gcggacgcaa aaagtgtttg atcaagtgac ggattactat ctgaagaacg agaaagcgaa
     3181 cgttgaaagt gtctttacgg ttaacggctt tagcttcagc ggccaggcac aaaacgccgg
     3241 tatggccttc gtcagtctga aaccgtggga agagcgtaat ggtgatgaaa acagtgcgga
     3301 agcggtaatc catcgtgcca aaatggaatt gggcaagatc cgcgacggtt ttgtcattcc
     3361 attcaatatg ccagccattg ttgaactggg cacggcaacg ggtttcgact ttgagttaat
     3421 tgatcaggct gggctgggtc acgatgccct gacccaggcc cgtaaccagt tgcttggcat
     3481 ggcggcgcaa catcctgcca gcttagtcag cgtgcgtcct aatggcctgg aagacaccgc
     3541 gcagtttaaa ctggaagttg accaggaaaa ggcgcaggca ttaggtgttt cactttctga
     3601 catcaatcag accatttcaa cggcgttggg tgggacttac gttaacgact tcatcgaccg
     3661 tggtcgcgtg aaaaaggtgt atgttcaggc ggatgccaaa ttccgtatgc tgccagaaga
     3721 tgtcgataaa ctttatgtcc gcagcgccaa cggcgaaatg gtgccattct cggcctttac
     3781 cacttcacat tgggtgtatg gctctccgcg actggaacgc tacaacggtc tgccgtcaat
     3841 ggagattcag ggggaagccg cgccaggaac cagttccggc gatgccatgg cgttgatgga
     3901 aaaccttgcg tcaaaattac ctgcgggcat tggttatgac tggacgggta tgtcgtatca
     3961 ggaacgctta tcgggaaacc aggctcccgc tctggtagca atttcctttg tggttgtttt
     4021 cctgtgcctt gctgcactct atgaaagctg gtcaattcct gtctcggtta tgttggtagt
     4081 gccgttaggg attgtcggcg tgctactggc ggcaacactc tttaatcaaa aaaatgacgt
     4141 ctactttatg gtgggcttgc taacgacaat tggcttgtcg gccaaaaacg ctattttgat
     4201 tgttgagttc gctaaagatc tcatggagaa agagggtaaa ggtgttgttg aagcgacact
     4261 gatggcagta cgtatgcgtc tgcgtcctat cctgatgacc tctctcgcct ttattctcgg
     4321 cgtattaccg ctagctatca gtaacggtgc cggcagtggc gcgcagaacg ctgtgggtat
     4381 cggggtaatg ggaggaatgg tctctgcaac gttgctggca atcttcttcg taccggtgtt
     4441 ctttgtggtg atccgccgtt gctttaaagg ataaatcaga aacataaagg cgctttcggg
     4501 tgcctttatt atttccagtg aaacccataa aaattaataa gatattcttc tgctcactct
     4561 ttaaaagctt tctatagttc ccgctccctt cactattttt acaattcaca taatttgtca
     4621 ttattcattc cgcaagattt ataacctccg gtgatataat ggcattcagc tcgttaataa
     4681 gagagttaac ttattaagcg ttagcgttta ttactgaggt aacaccatga aaagattaat
     4741 tcctgtggca ttgctcaccg cattgctggc gggctgtgct cacgattcgc cctgcgttcc
     4801 g
//
LOCUS       DAPGWD010000231         4777 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPGWD010000231 DAPGWD010000000
VERSION     DAPGWD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.233"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..596)
                     /locus_tag="RHX88_004227"
     CDS             complement(<1..596)
                     /locus_tag="RHX88_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDV2232837.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="RHX88_004228"
     tRNA            complement(711..786)
                     /locus_tag="RHX88_004228"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="RHX88_004229"
     tRNA            complement(793..867)
                     /locus_tag="RHX88_004229"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(984..1068)
                     /locus_tag="RHX88_004230"
     tRNA            complement(984..1068)
                     /locus_tag="RHX88_004230"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Tyr,seq:gta)
     gene            complement(1077..1152)
                     /locus_tag="RHX88_004231"
     tRNA            complement(1077..1152)
                     /locus_tag="RHX88_004231"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1117..1119),aa:Thr,seq:tgt)
     gene            1514..2464
                     /gene="coaA"
                     /locus_tag="RHX88_004232"
                     /gene_synonym="panK"
     CDS             1514..2464
                     /gene="coaA"
                     /locus_tag="RHX88_004232"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDV2232838.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3458)
                     /gene="birA"
                     /locus_tag="RHX88_004233"
     CDS             complement(2493..3458)
                     /gene="birA"
                     /locus_tag="RHX88_004233"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDV2232839.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEEILSQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3455..4483)
                     /gene="murB"
                     /locus_tag="RHX88_004234"
     CDS             complement(3455..4483)
                     /gene="murB"
                     /locus_tag="RHX88_004234"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDV2232840.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1208 a         1216 c         1088 g         1265 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtggccga aattggtggt gggggaagga ttcgaacctt cgaagtctgt
     1021 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccgg acttgatggt
     1081 gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctactg
     1141 agctaagtcg gcatcaagta gcgcgcattc tatggagaca tgcgagttca tgcaactaaa
     1201 aaattgcata atttgtttta ttggtcacat tttatgcgac acgatgaaga aacagccgaa
     1261 cggagggcag attagcacac tttttcaaca tcattgtgct caacaatgcg ctcctgctaa
     1321 accataattc tttttatcag atggaatatc tgtcacattg cttttcaacg atagcttcct
     1381 ggcagagatt ttttcttatt attcctcccc atctggtgtt accctcctgc ccattaaccc
     1441 attcaacaga actgtgacgc gccatggcaa atatcgcttt gccgatagag ctatgaccgc
     1501 cagaaacatg cttatgagta taaaagagca aacgttaatg acgccttacc tacagtttga
     1561 ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg acgttatcgg aagatgagat
     1621 cgcccgtctc aaaggtatta atgaagatct ctcgttagaa gaagttgccg agatctattt
     1681 acctttgtca cgtttgctga acttctatat aagctcgaat ctgcgccgtc aggcagttct
     1741 ggaacagttt cttggtacca acgggcaacg cattccttac attatcagta ttgctggcag
     1801 tgtcgcggtg gggaaaagta caaccgcccg tgtattgcag gcgctattaa gccgttggcc
     1861 ggaacatcgt cgtgttgaac tgatcactac agatggcttc cttcacccta atcaggttct
     1921 gaaagaacgt ggtctgatga agaagaaagg cttcccggaa tcgtatgata tgcatcgcct
     1981 ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac gttacagccc ctgtttactc
     2041 gcatcttatt tatgatgtga tcccggatgg agataaaacg gttgttcagc ctgatatttt
     2101 aattcttgaa gggttaaatg tcttacagag cgggatggat tatccacacg atccacatca
     2161 tgtatttgtt tctgattttg tcgatttttc gatatatgtt gatgcaccgg aagacttact
     2221 tcagacgtgg tatatcaacc gttttctgaa attccgcgaa ggggctttta ccgacccgga
     2281 ttcctatttt cataactacg cgaaattaac taaagaagaa gcgattaaga ctgccatgac
     2341 attgtggaaa gagatcaact ggctgaactt aaagcaaaat attctaccta ctcgtgagcg
     2401 cgccagttta atcctgacga aaagtgctaa tcatgcggta gaagaggtca gactacgcaa
     2461 ataattttca ggggagctaa tgctcccctt tcttattttt ctgcactacg cagggatatt
     2521 tcaccgccca tccagggttt tattattcca tcctgctcaa gcaataaagc gccctgtttg
     2581 tctattccgc gtgaaatgcc aaatatttct ttatcaccaa tgataagttt cactgggcga
     2641 ttaataaaat tatccagctt ttcccagcgc gaaagataag gtgccaatcc ttcttgttcg
     2701 aagagttcca gcgccgcgcg taattcacgt attagcatgg ccgccaacgt attacgatcg
     2761 agattgatcc ccgcttcctg cagcgtgatc cacccctgat taacgacact ctcttcaaca
     2821 cgacgcattg ccatgttgat cccggctcca atgactattt gcgccgcatc gccggttttg
     2881 ccagtcagct ccacaagaat gcctgccagc ttgcgatcct gcagatagag gtcattaggc
     2941 catttaacac gaactttatc tgcacccagc ttgcgtaata cttccgccat cacgataccg
     3001 ataaccagac ttaaaccaat agccgccgcc gggccttgtt ccaaacgcca gaacatcgac
     3061 aaatataagt ttgcgccaaa aggcgaaaac catttccgac cccggcgacc acggccagcc
     3121 tgctggtatt ctgcaacgca ggcatcgccc gatttaagct ctccgatacg atcaagaagg
     3181 tactgattcg tggagtcaat cactggcagc acggctacac taccgccatc cagctgactc
     3241 aatatctctt cagcattaag taactggata ggttcaggca ggctgtatcc tttacccgga
     3301 acggtaaaga catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt aatagccgcc
     3361 cggctcattc ccagcgtttc acccaactgc tcgccagagt gaaattcacc gttcgctaac
     3421 agggcaatca atttcagtgg cacggtgtta tccttcatga aattgtctcc actgcgctca
     3481 cttcacctga tgcaccaata aagcggactt caggctcaag ccagacatta aatttttcac
     3541 ccactttttg tcttacatgg tgtgccagtt gcaccacatc ttcgcttttt gcattgtctt
     3601 cattaatgag aactaacgcc tgttgacggt gcaccgcagc cccacccatt tgcatccctt
     3661 ttagctggca ctgatcaata agccaacctg ctgccagttt tactgaacca cccgcctggg
     3721 ggtaatttgg tgctgttgga aattgcgcca gtaatgcttt agccgtttcg gcagatacaa
     3781 cagggttttt gaagaaacta ccggcattgc cattcacttt tggatcaggg agtttggtgg
     3841 tgcgcatatg acacaccgca ttaaatactt gctgtggcgt tactgttgta ggatccagac
     3901 gagttaagtc accatacgtt agtacaggtt gccactcttt tggcagacgc agacctacgg
     3961 ctacaatggc gaagcgatcc tggtattcat gtttaaaaat actgtcgcga tagccaaaac
     4021 ggcactcttt ggcagttaag cgcacttgct tgcctgtcgc cagttcaaca caatcaacat
     4081 aagcgcaaac tcgctgtaat tctacgccat aagcaccaat attctggata ggtgatgagc
     4141 cgacacaacc aggaattaat gccagatttt ccagaccagg cataccttcc tgcaaagtgt
     4201 attttaccag gcgatgccag ttttctccgg ctcctacatg taaataccac gcatcaggtt
     4261 catcatgaat ttcgatacct ttgatccggt tgatgatcac cgtgccgcga tagtcttcca
     4321 gaaaaagtac attacttcct tcacccagaa taagaacggg ttgtccttct gcggttgcat
     4381 actgccaggc attgagtaat tgttgttcgt cttcggcaca tacaatgtgc tgagcattat
     4441 gatcaatgcc aaatgtgttc cagggtttta aggagtggtt catagctgct ttcctgatgc
     4501 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa
     4561 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     4621 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca
     4681 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4741 aacgaaaggc ccagtctttc gactgagcct ttcgttt
//
LOCUS       DAPGWD010000232         4776 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPGWD010000232 DAPGWD010000000
VERSION     DAPGWD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.234"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(49..810)
                     /gene="kduD"
                     /locus_tag="RHX88_004235"
     CDS             complement(49..810)
                     /gene="kduD"
                     /locus_tag="RHX88_004235"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDV2232841.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(840..1676)
                     /gene="kduI"
                     /locus_tag="RHX88_004236"
     CDS             complement(840..1676)
                     /gene="kduI"
                     /locus_tag="RHX88_004236"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDV2232842.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1963..3144)
                     /locus_tag="RHX88_004237"
     CDS             complement(1963..3144)
                     /locus_tag="RHX88_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDV2232843.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3399..4628
                     /locus_tag="RHX88_004238"
     CDS             3399..4628
                     /locus_tag="RHX88_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV2232844.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1236 a         1224 c         1051 g         1265 t
ORIGIN      
        1 ataaaaaacc ctgccatgcg gcagggtcat aaaagtaaga agaatgaatt aacgcgccag
       61 ccaaccgcca tccacggcaa tggtataacc attcacataa tctgaagcgc tggaggcaag
      121 gaacactatc ggccccatca ggtcactcgg cagtccccaa cgaccagctg gaatgcggtc
      181 gagaatttcc gcgctacgtt gttcatctgc ccgtagttgt tgagtattgt tggtcgccat
      241 gtaacccgga gctatcgcat taacattaat gttgtgttta gcccattcgt tcgccatcaa
      301 tcgcgtcaca cccatcacgc cgctttttga tgcggtataa gaaggcacac ggatcccgcc
      361 ctggaaggag agcattgacg cgatattgat aatcttgccg ccattgcctt gcgcgataaa
      421 gtgtttcgcc gctgcctgag acatgaagaa tacgctcttg atattcaggt tcatgacatc
      481 gtcccagtct ttttcgctga actcgagagc gtcttcgcgg cgaatcaatc cggcgttatt
      541 caccaggata tcaatatgac caaattcagc taccgcgcga tccagcagtc caggaatgcc
      601 atcaatcttt cgcagatcgg cggtcaggct taaaaaacga cgccccagcg ccgtgacctg
      661 ctcgatggtt tcagtcggtt caacgatgtt aatgccaaca atgtcacagc ccgcttgcgc
      721 cagccccaac gccatcccct ggcccagccc agtatcacaa ccagtgacga ccgcaacttt
      781 accttcgaga gaaaatgcac ttaaaatcat aacaatacct tattcgttta tgcccacaac
      841 tagcgcaaat ctttaacggc cacatggtcc atatcatcaa agacctggtt ttcaccgacc
      901 atgccccaga taaaggtata agctttggtt ccgacgccgg aatgaatcga ccagctcggg
      961 gagatcaccg cctgctcgtt atgcatcaca atgtgacgcg tttcttgcgg ctgccccatc
     1021 atgtggaaaa cgcaggcgtc atcatccata ttgaaataga aataaacttc catccggcgc
     1081 tcgtgagtgt gacacggcat ggtgttccac aagttacctg gagccagctc cgtcagcccc
     1141 atactcaatt ggcaggtttc cagcacatcc gggacaaaat atttgttaat tgtgcgacgg
     1201 ttactggtga ggttatcgcc taacgtgact ggagatactt cgtccggtgt gacttttttg
     1261 gtgggatacg tcgtatgcgc gggtgcgcaa ttgtaataaa actttgccgg agtggcggta
     1321 tcaatactgg caaagacaac ttcttttgca cctttaccca catacagggc gtcgcggtga
     1381 ccgatttcat agcattggcc atcgacagta atcgtaccgg caccgccaat attgataaca
     1441 cctaactcgc gacgttcaag gaaatagctt acgccgagtt gtttaccaac ttccccgcca
     1501 acggaaaccg ttttagttat cggcataatg ccgccaacaa taattcggtc aatgtggctg
     1561 taaaccatgg tgtactcatc ggcgacaaat acctcttcaa ccaaaaattc attgcgcagc
     1621 ccttgggtat ccagcgtttt tgcgtgcgca ctgtggatgc tctgtcttac gtccacatca
     1681 acctccgata atcagtgaag cgtgaaaata gaaaacgata gtgaaacact gtttcgtttt
     1741 ctctataaag ggatgatatg cccatgggct agggttttca attaaaaata aaacaatgtt
     1801 tcatttttaa gttaaggatt aaaaaaagtg cagtgtcgat cacgaatatg tcatgagaat
     1861 ggaaaaatga gggagcccag aaaatgtatg tcgacttgcc agccatcatt attcaacaga
     1921 ttaatgacgg cagatattta tcggtaagga ggaaatgcag agctactcgt cacgttcaat
     1981 ggtcaatgcc acgccctggc ccccgccgat acaaagcgtt gccagccctt tgcgggcatt
     2041 acgtttcacc atttcatgaa ccagagaaac caggattcgg caaccggaag cgcctatcgg
     2101 gtgaccgagt gcgatcgcgc caccattgac attgacccga cgctcatccc actcaagcat
     2161 cttgccaacc gaaagcgcct gtgcagcaaa cgcttcatta gcctcgataa gatcgacatc
     2221 agccaactgc cagcctacac gctccaggca acggcgggtc gcatacaccg gcgcaattcc
     2281 catcaatgcc ggatctacac caacgctggc aaatgcgcgg atgcgggcca gcacgggtaa
     2341 attcaacgct cgtgctttgg cttcgctcat catcattacc gcagctgcgc catcgtttat
     2401 ggatgatgca ttccccgcgg tcacagaacc gagactatca aatgaaggat ttaaacgggc
     2461 taaaccttct gcactggcgt cagtgcgtgg ctgttcatcg gtatcaacaa ccaacgtctg
     2521 accgttactt tgggtcatta ccgggacgat ctcatcttta aatcgtccgg cgtcaatcgc
     2581 cgctcgcgct ttttgttgcg agctaagtgc gtaagcatcc tgcaattgac ggctgatgcc
     2641 atattcgcga gccagatttt cggcggtgac accaatatga taatcattga aggcatccca
     2701 taacccatca tgcacaagac tgtcaaccag ctggctattg ccaagctgtg caccggtgcg
     2761 gctatcagtc agaacatgtg gtgcgcggct catgttttcc tggccaccgg cgatgacaat
     2821 atcagcctcg ccacactgta tcgcctgagt agccagatgc agtgctttaa gtccggaacc
     2881 gcagacgtca ttaatcgtga ttgcagaaac gctattaggc agaccacctt taatagccga
     2941 ttgccttgcc ggattctgcc ctgcccctgc agtcaacacc tgaccaagaa ttacttcatc
     3001 caccgcatat gcaggaacgc cggtacgttc tattaacgct ttcacgacca gactaccaag
     3061 ttccacggcg gaatgacccg ctaacgcacc gcgaaagcag ccgataggtg tccgtaacgc
     3121 cccgacaatc acaacgtctt tcatcacaac ctcgcaccaa ttaacagtgc aatagtaaat
     3181 gattgttaat tacagttatc ttaattgttt aaaaaaagtg atttttatca caaaggaaat
     3241 atgcctgagc agtagtcaga gtcataactg gcacgtaagg tttgcaacca ctaacccacc
     3301 aatagagggg tagatagggc gttaatctcc catacttaac ctggtttatg gtaaattgcc
     3361 ctccattttg tttaatttgt agatgatacg ttcagataat gtctaatatt tggtctaaag
     3421 aagaaactct gtggagtttc gcgctctacg gcacagccgt tggtgcaggc acgctcttcc
     3481 ttcctattca gttaggttcg gcaggggctg tggtcctgtt tattactgct ctggtcgcct
     3541 ggcctttaac atattggcca cataaagcct tatgccagtt catcctctca tcgaaaacat
     3601 cagcaggtga agggataacg ggcgcagtaa cacactacta tggcaagaag attggtaatc
     3661 tgattaccac gctgtacttc atcgcctttt ttgtcgtcgt gttgatatat gcagtggcaa
     3721 ttaccaactc acttacggaa cagctggcaa agcatatggt tattgatctt cgcatccgta
     3781 tgttggtgag tctgggtgtt gtattaatcc tgaatctcat ttttctgatg ggacgccatg
     3841 caaccattcg ggtaatgggt tttttggtat tcccattgat tgcctatttc ttatttcttt
     3901 ccatttacct tgtcggtagt tggcaacctg atctattaac gacccaggta gagttcaatc
     3961 agaataccct tcaccagata tggatatcga ttcccgtgat ggttttcgcc tttagccata
     4021 cgcccattat ttctacgttt gccatagaca gacgtgaaaa atatggcgaa cacgctatgg
     4081 ataaatgcaa aaaaattatg aaagtcgctt atctcatcat ctgcataagt gtactgttct
     4141 ttgtctttag ctgcctgctt tctattccac cttcgtatat tgaagcggct aaagaagaag
     4201 gggtcaccat tttatcggcg ctttctatgc tgccgaacgc cccagcatgg ctgtcaattt
     4261 ccgggattat tgtcgcagta gttgcgatgt cgaaatcatt cctgggtacg tactttggcg
     4321 ttattgaagg tgccacggag gtcgtcaaaa caacactaca gcaggttggt gtaaagaaaa
     4381 gtcgtgcatt taaccgcgca ctatcaatta tgttggtatc gctgatcacc ttcattgttt
     4441 gttgcattaa cccgaacgcg atttcgatga tttacgcgat cagcggcccg ctcattgcca
     4501 tgatactttt catcatgcct acgttgtcaa cgtatctgat cccggcgctt aaaccctggc
     4561 gttccatcgg aaatctgatt acgctgatcg tgggtatcct gtgcgtatcg gtaatgttct
     4621 ttagctgata tccattgtga atggttcaaa attgctacaa ttttgaacca ttccagtttt
     4681 cataatgttc attcgttatt attccgcact atcacttccc tcatcatatc ctagtattat
     4741 cagttagtta aacacacaaa gagatctgaa catctt
//
LOCUS       DAPGWD010000233         4749 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPGWD010000233 DAPGWD010000000
VERSION     DAPGWD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.235"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(64..822)
                     /locus_tag="RHX88_004239"
     CDS             complement(64..822)
                     /locus_tag="RHX88_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDV2232845.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="RHX88_004240"
     CDS             complement(830..1933)
                     /locus_tag="RHX88_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV2232846.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3124)
                     /locus_tag="RHX88_004241"
     CDS             complement(1943..3124)
                     /locus_tag="RHX88_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV2232847.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3192..4217)
                     /locus_tag="RHX88_004242"
     CDS             complement(3192..4217)
                     /locus_tag="RHX88_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2232848.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPAVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1309 a         1236 c         1116 g         1088 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgcagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggctcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccaccagaaa tttgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc tttggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtagaac ggtcagatga gggaagagat tgaaatgctg
      541 aaagaccatt cccacttcct gccggacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct accacgatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac ctgaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttattga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gctggctata gcgcgacatg ctgaaacaga
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggtgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caacccgata atgatcacca
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccgcc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atattggtgc
     1261 cttctgccat aaacaaaggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa cgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctcc agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag accgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gattgacaca aggccaatca
     1621 gtaatgccag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgctgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aatcactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaatcc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagatg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggtg cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcctgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg cagcagcggg ggaatattac ggaagatctc aatataaatt gtggatagtt
     2761 ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagcct aaaacagaag
     2821 cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc
     2881 gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
     2941 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt
     3001 tgtgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc
     3061 aggcgcgaac cgtagggttg gcaaaagaga gtgagccttt aacggttgag cggcgatgag
     3121 acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgcccgttac
     3181 ctcacaccga cttaacgcac gggcggcgcg tactgaatac cgccgttatt ccagagatta
     3241 ttttgcccac gtttaatttt cagcgggctt tctgaaccta cgttacgctc gaaaatttcc
     3301 gagtagttac ccacctgttt gatgatgtta taggcccatt tattatccag cttcagatcc
     3361 ttgccgtaat cgccttcttt acccagcaga tgtgccatat caggcgttgc tggattagcc
     3421 gctttttcat cgacgttctg ggaattgatg cccatctctt cagcattcag catggcgaaa
     3481 agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactgc cggcccaagc
     3541 ggttctttag agataacctc cggtaagaca atccattcag ctgggttgct taatttgatg
     3601 cgcagggcat acagttgcga ctgatccgag gccagcgtat cgcagcgacc agattccagt
     3661 gccttcgctg attcgtcaga gcgatcgaaa gtcaccggtg tgtacttcat attgtttgcc
     3721 ttgaaatagt cggcaacgtt gagttcggta tcagtacccg cctgaataca gacggtagcg
     3781 ccatccagtt ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa gccaatgccg
     3841 tcgtaataag tgacgccggt aaatgccatt cccatcccgg catcgcgaga tgaagtccag
     3901 gtcgtattac gggagagcaa atccacttcc cctgactgta aagcggtgaa gcgttctttt
     3961 gctgtgagtg gggtatattt cactttcgtg tcgtcaccaa atacagcagc ggcaacacca
     4021 cgacaaacat caacatcaat acctgaaaac ttaccgtctg catcggcata agagaaccca
     4081 ggtaatccat cactgatccc gcattgcaca aaaccttttt tctgaacggc atcaagcgtc
     4141 gcgccagcat gcgcctgatt tgcaacggca agcagcacgc tggcggcagc cagtgtggct
     4201 atcatcatct ttttcataat gcttcctgtg aggcgaaaat tatcgttata ggacgtttgg
     4261 gaaacgtctt ttacctgtct gtggccatcc tcttataagc aaaagtagtg ccagcttttg
     4321 cgcccgattt cgcgtcgcaa taagctgagt gtagacagga aataataaaa acagcgcccc
     4381 gcaatggtgc gaaaatgaaa catgcgccac attttggcgc aaaatattta cgcgtgtttt
     4441 actaagcaga ataatgattg ttagcgtgag tatatttctg gtgggaagtg tgaaagttaa
     4501 attgatgcgt tatttcgaaa gcagcgaaat aacgcgatat aacatgctaa atcagcagag
     4561 aattagccag cagaatatag caatatttta tcttatgtaa agatactgta tcgttaaaca
     4621 gagcgcgaaa ctccgcgccc tgttcagtta tttcgacaga gcgatgatcc ccatcgtcag
     4681 gccagcaaca gccgctgccc cgccagcaat agccccagcc gtttcccagt tatcctgagt
     4741 cgtgccttt
//
LOCUS       DAPGWD010000234         4653 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPGWD010000234 DAPGWD010000000
VERSION     DAPGWD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.236"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(318..791)
                     /locus_tag="RHX88_004243"
     CDS             complement(318..791)
                     /locus_tag="RHX88_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232849.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     ASRLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
     gene            complement(908..1756)
                     /locus_tag="RHX88_004244"
     CDS             complement(908..1756)
                     /locus_tag="RHX88_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDV2232850.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKCPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            complement(1796..2077)
                     /locus_tag="RHX88_004245"
     CDS             complement(1796..2077)
                     /locus_tag="RHX88_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2232851.1"
                     /translation="MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(2074..2343)
                     /locus_tag="RHX88_004246"
     CDS             complement(2074..2343)
                     /locus_tag="RHX88_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232852.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            complement(2398..2556)
                     /locus_tag="RHX88_004247"
     CDS             complement(2398..2556)
                     /locus_tag="RHX88_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232853.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISW"
     gene            complement(3252..4283)
                     /locus_tag="RHX88_004248"
     CDS             complement(3252..4283)
                     /locus_tag="RHX88_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV2232854.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
BASE COUNT         1013 a         1270 c         1163 g         1207 t
ORIGIN      
        1 ggtttctcga agtgacgagc gatcagaatg gagacaaata aattcgggaa acgaaccttc
       61 acggtaagtt ttgtcacatt tatcatccct aaacatgaga attatcacca aaactaacca
      121 cccaaccaac agcagcgccg ggagcagcat tataaaatca tgtacagagt tgcgtgacga
      181 catgataagt tctcattctc aatatgttct ttaccagatt ataccagtga tactttaacg
      241 caccatcatt aatgctaatg tatgcataca ttaacattaa tgatgaagga gaagaacaac
      301 aatggcagtc aacccactta ttttttggat ttcttaattt tcgtctgata aagccgcttt
      361 acccgttcaa tttctgccag cgttgcggac cagtgtagtt cggcggcatg acgatatctg
      421 gcagccattt tttccaccat ctccggattg ctgtccgcgc tgcggtgctg acgccccttc
      481 tccccaaaaa cttcaacaac cagccactgg cttccggttc tgccgaacaa tgccttgcac
      541 aaacgtctca cagttagcgg ggttaaatct ggatcgggat cgtaggcaat ggtagcaaaa
      601 ataaagcgca gcatttctga tgtcttgtaa cgctttacgc tggcagccag gcgtgaggca
      661 cgttgcggtg cttcccagcg gtcgttactg taattagcca cttcagccag ataatccttt
      721 tcaagttttt cagccgtgta gcacctgccg cctaaaaaaa gccgcatggc ctccctggtt
      781 ttgtctctca catatcatcc ctcattaatg ctaatgtaca catacattag cattaacatg
      841 ctgacagcaa caagcggcgg atttacgtct ggataacgta aatgattgcg gtatacaggc
      901 caggcggtca gattttcagc cgtgcctgtt cctgcagcag ttccagatga tatgcagcaa
      961 tagcgtttac cacccctgcc gtcatccgtg cgtcggcgat cgctgagtgt gcctgccctt
     1021 cccaggtcag tccggcctga ctggcagcac aggccagtga aatcgtgcca tagcggttgg
     1081 tggccccata atatcctgca gccagctcca tcgcacaata taccgtcagt tcttccagcc
     1141 agtcagccgg atcgctatgt gcggcagcag tctgtttcag aatgcggatg tcgaaccggg
     1201 cattaaagat aattactggt cggtccccga ttgcatgacg cagttgccgc gccacatctg
     1261 tccatgatgg tgcgccgcag agagcctgct cgctgatacc atgcacagcg gcggcctgcg
     1321 ccccgatggc gaccgtgggc ttcagcctgg tttcaaatac cacctgaccg gccgcatcgg
     1381 tcaggccgat ttccagggct tccgcggtat tatccagtcc tgtcgtttca gtatccagaa
     1441 agagcggtgc cagactcagc cagtcatatg cctgccgtgc catccggcca ctggtgctgt
     1501 taagtctgga taaaacggaa agtcgctggc cagccagctg ttgtttactc gtcagagggc
     1561 atttctcacg catcggaaca cagtctgcaa ttcggtatac agcgaacctg ccaccataag
     1621 tgtttttata ccacttaacc ggctcagcac cgggggctgg tttcattctg aattcatcgc
     1681 gcaaacgtcc tttcgagaaa cacctgtctt cggcaatcgc ctgcgcacgt ctggtggcca
     1741 gctcatcatt attcatggtt attgcatctc ctttccttat atccggacag cccctctatg
     1801 gccacttctg tgcagtatgc aacacgcgca ggatatacac ttttccccac tggctgtcga
     1861 cctcataaac cagaacaaaa tgcgggtgaa taaccagttc ccgggtacct tccacacgac
     1921 cgttgcggcc tttatacggc tgcccggcca ggctgctggc tgaataacca atccgttcgt
     1981 ccagttcaat ggcggccatc aggttctgct ccgcaatgta cttacgaatg cgcttcctgt
     2041 cctggctggc cagcatggtc cagaatatct ccatcattta ccggcaacct tcctgctcat
     2101 ttcatcccgc catgcagcag cggaagattc gaccatgtca tggggaagca catcgccgcg
     2161 ctcgagttgc tgacgtcctg cctcaacctg gtcacggaac caggtattat gctgccgttc
     2221 aacggtctgg cgcataaaat cccgaattaa ctgagagcca ttacggtcca tgctttttgc
     2281 agcggccata aaagcatcct tcagttcagc gtcgattctt aaactcatat taacctgtgc
     2341 catttatcac ctcgtcatta ctacgtatat acacagtata taacgattca gatatcgtca
     2401 ccaggatatg ccgcgccagc ggcatggaag gcggcacgct gtggttacat gtgataccgg
     2461 agtaaaaccg ccgaagcccg gcgtcagccg gtactgattg atagatttca ccttacccat
     2521 ccccagccct gctaagccat acccgctttc agccatgaga gagtttctgt gcgcggtcgg
     2581 agtggtcccg acgagggttt acccgaagtc ggggcgtatc tccgcgttag cgggccgtca
     2641 gggccgctta cgagcgtgta ctgagaacat ccagccagaa gactgacagc gatgataatg
     2701 tagttacaac attcataatt aaaagcgatg ttgttcccgc cctttgggcg ggggcggcct
     2761 cgcttttcag ttatgagggg tgggcacggt gctgcgtttg tgtggtgttt ttattcctga
     2821 tgtctttttg ttgtggtttc agaacggtga ggttcggggc ccgcgcgtca gcgtgggagg
     2881 tgcttttaga ttctgagcgg cgcggccttg gccgctgcaa gcgaaggcct ctttgtattt
     2941 gttcacagtg gtctcagaga taaagatttt ccctgcggcg aagcaacgca ggggattaaa
     3001 taatggggtt aagactatat tgcaggtttg ctgtttcagg tttgcagctt cttaaagttc
     3061 tttgtttgcg gatttatgtg gataactgaa acaacgtggc catgttctga tgactgatgc
     3121 gtggttttat gggcagatat gcccctctga ggggatttaa ggtgattact gcggattttg
     3181 ggctgagcgg aaccagcgcc gaaagccccg gaggggctgg tgatgattca gtgctgaggg
     3241 aatggtgctg tctagtgcgg tggcggggca gccagcgtca gttcaagctg acgcaactcc
     3301 ctgatggcca ttcgcttcag atggtcatcg gaacaaaaat aggcttcatc cggaggcaga
     3361 cgcttcctga gatactctgc catctcatga atctgctggt cggcaggcag tttcttcagg
     3421 cgtctggcgc gcttactggc tgctgctttc gcacgacggt gtttcagcgc atcctgactg
     3481 cgctgcagat accagcgttt tctggccgca tgtacggaga tatcttcatc ctcacgcaga
     3541 acaccttccc gaatgagctg ctggcggatt tcactttcac gcagtcgttt ctgctgctgt
     3601 tcgtgaagtt ttaccatgtc gacgcccagc atctgccagc ctgccggtgt tatccagacg
     3661 taacgtggca gacgctggcg ggtttcacgg tcccacaggg tttcctctga aacacccagc
     3721 acaccaaaac gtacctgttc ggccagcaaa cgggaaaggc gggagaccgt cacttccagt
     3781 tccggaataa ccttcccctt gctgtctttc gggctgattt ctctggccag acgggaaaca
     3841 ctcatgccta cggtatgtgt gcccgcatca ctgaagctga ccagaaccgg ccagacggca
     3901 tcgaggaggc gttgtttttc cggacggaaa gcatatttgc gaccggcata cgttcgtttc
     3961 tgcacgtaaa aaggatgcag agacatgtgc atgcgcaggc tctgctcgcc ggtcaccggg
     4021 tcctttttca ccagacggtt gtaggcgtgc ccgagctgcc cgctcagcgc cttatagcgc
     4081 tcaggacgat accaggacgg attttcgcat ttgggtttgg tggagtgctc accacgacga
     4141 cgcttcgtgc gcttcctttc cggttcaacc aggaaagtgg tcgcccttcc cgcttcatag
     4201 tcttcccaga aacggatctg ctcttcgggt gctaactgac tccagtgtac tgcgttatat
     4261 ggagtattct tcagacctgc cacaagctgt cgcttatggc ggagcgattg tgccgtgtat
     4321 ttatacactg cacagggttg aatctctggt acggtttcat tatactttac ccgtattctg
     4381 tgaggccccc actatctttc gtcgttccgc caagttcgta agaaagatag tgggggtttt
     4441 ccttttatgg cttccggaac atcttcagtc ttctgactta ctgcccgtac atgccatctc
     4501 tcctgaatgc cttatggcgc attctgccgt ccggctttcg ccgctttgct ccacagagtg
     4561 ccaccctctg cacagctaat aacagccgaa atatacccgc tttacgttaa tttgaaaagc
     4621 attacaacga tctcttctct tccagtgcct cct
//
LOCUS       DAPGWD010000235         4587 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPGWD010000235 DAPGWD010000000
VERSION     DAPGWD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.237"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..317)
                     /locus_tag="RHX88_004249"
     CDS             complement(<1..317)
                     /locus_tag="RHX88_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDV2232855.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKR"
     gene            421..942
                     /locus_tag="RHX88_004250"
                     /pseudo
     CDS             421..942
                     /locus_tag="RHX88_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
     gene            complement(1072..3042)
                     /locus_tag="RHX88_004251"
     CDS             complement(1072..3042)
                     /locus_tag="RHX88_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDV2232856.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(3053..4453)
                     /locus_tag="RHX88_004252"
     CDS             complement(3053..4453)
                     /locus_tag="RHX88_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2232857.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
BASE COUNT         1103 a         1171 c         1241 g         1072 t
ORIGIN      
        1 cgtttttgct cacggatatc agaccagtca acaagtacaa tgggcatcgt attgcccgaa
       61 cagataaagc tagcatgcca acggtataca gcgagtcgct ctttgtggag gtgacgatta
      121 cctaacaatc ggtcgattcg tttgatgtta tgttttgttc tcgctttggt tggcaggtta
      181 cggccaagtt cggtaagagt gagagtttta cagtcaagta aggcgtggca agccaacgtt
      241 aagctgttga gtcgttttaa gtgtaattcg gggcagaatt ggtaaagaga gtcgtgtaaa
      301 atatcgagtt cgcacatttt gttgtctgat tattgatttt tggcgaaacc atttgatcat
      361 atgacaagat gtgtatctac cttaacttaa tgattttgat aaaaatcatt aggggattca
      421 tcagaactta gcctgaccgc atcctggatg ccgctggaat ttgaccctgc cgataatgac
      481 gattatgcca tgcaacagct tgataagcgt gatagtacgg ctatggcggg ggttgcctgg
      541 tatcaccacg agcgttgggg aaccgtgaaa gcctctgcag ctgcggacgt tctggataac
      601 agcaacggct gggtggggga gctatcggta ttccacaaaa tgcagatagg tcgtctgtcg
      661 ctgacacctg cgctgggcgt tctctattat gacgagaatt tcagtgacta ttactatggc
      721 atttcagaga gtgagtcccg tcgtagcggt ctggtaagtt attccgcgca ggatgcctgg
      781 gtgccctatg tcagcctgac ggcaaaatac ccgataggag agcacgtcgt attgatggcg
      841 agcgcaggat acagcgagct gccggaagag gttaccgaca gcccgatgat tgatcgtaat
      901 gagagtttta cctttgtcac cggcgtgagc tggcgttttt gattcaccgg tggatgtcgg
      961 tgcggctggc ctacgctctc gcaccgactt gtatgcctga taagacgtgt taagcgtcgc
     1021 atcaggcaat gtgctcctta cgttggatgc ggcgcgagtg ccttatccaa cctacggatg
     1081 gcaatgcttt tcctcattca cccagatccg catttcgcct tcaccccggt tggcccagct
     1141 aaaccacggg ataaaggtca gagtttgcgt ctggcgtttg gctggcgcgc tgtcgtaatg
     1201 ccacagcggt tgctgctctg gattgctctg ttcataccgg taacccggtg cctggattaa
     1261 gatcttatgg ctaaacaatc ccttgccttc aaatgtcgta aatggcgcat cggtgggcag
     1321 ccacagatta tgcagtgact cgccgttgtc ggcctgttcc aggcaataca ccagcgggcc
     1381 gcgctgaatc gccacttttc cggcgacgtg acgcaccagc gggttaccgt aaacgcggcg
     1441 taccggcatc ggcaaagtca gattcagcgt atcgccctcc tgccattcgc gggtaatgtg
     1501 caaataccct ttacgaatat cctgctcgac ctcttcccca ttcaatatga tctgcggctg
     1561 tgtgcaccag tccggcagac gtaaagccag cgtatgacgt accggctggg gcgattcaac
     1621 cgcaatcgtc acctgctcct gccacggata gttcccgcta acccgcaggc gcagcgtgcc
     1681 attttctacc ggcacttcca tgctgtttcc tgcgtatatg ttgatataca acgcatcttc
     1741 acgcggcgtg tagagataat gaccaatcga ggtcagcacg cgggcgatat ttggcggaca
     1801 acaagcgcag ccaaaccaac gctggcggat cggtttaacg tgatcgtaga tatggttgaa
     1861 tttcagcgat tttggatgta cttccagcgg attcacatag aagaaatgtt tgccatccag
     1921 cgccatgccg ccgagcacgg tgttgtacag tgcgcgctcc atcacatcgg catattgact
     1981 gtcgccttcc atttccagca ttcgtcgggc aaacatcatc aggccgatgg aggcgcaact
     2041 ttcagcgtat accgtgtcat tcggcagatc gtaatcgcta gtgaacgctt cgccgctgct
     2101 ttgcgagcca atgccgccgg taatatataa ctgacgctgg gccatattgt tccacagccg
     2161 caggcagtcc tgacgcttgc tgtcatcgtg acttaaacgc gcgagatgcg cgacgccggt
     2221 catcagatag acaaaacgta ccgcgtgacc gatggcagtt tgctgttgcg caaggggcaa
     2281 atgtgcctgg ctgtaggctt tgtctttcac catccacgcc gggccgtagg tgtgccagtg
     2341 cgatgtctgc ccgcgctttt catattcctg gtcgtaatag tgcggttgcg caccacgctg
     2401 ttcgacaaaa tagttcgtca gcgccaggta gcgcggctct tcagtcactt catacaggcg
     2461 cattagtgcc agttcaattt ccgggtgacc aggataaccg tgtaacttac tttcatctgg
     2521 accaaatacg ctgtcgatat gatcggccag gcggcaaacc acttccagca agcgccgttt
     2581 tcccgtggcc tggaagaagg cgactccggc ttcaatcaga tgacctgcgc agtaaagttc
     2641 atgacactcc gccagattgc tccagcgttc ttcgggtgct tttaccgtaa agtaagtatt
     2701 gagatagccg tcttcgcatt gggcggaggc gatcagttcg attacctcgt cggcggtttt
     2761 ttccagttcg gcgtccggct tctggcacag cgaccaggct accgcttcca gccatttggc
     2821 gacgtcgctg tcctgaaaca ccatcccgta aaattcaccc tcctgaagtc cggcggcaat
     2881 acgaaagttt tcaatcgcat ggctgggttc cgcttctggg atacgatcgt tcaaggcatc
     2941 ccactgataa ggaatcacca cgtcgcggac cagttgttgg tactgaccga ggaacggatc
     3001 gctgaccgtc agtttatgca gatcgacttc cgaaatgttc atcttgtgct ccttaaggct
     3061 gtacatgacg ctgacgcagg tcggtagcta tgcgtgacat cataggatta ttgagtttgc
     3121 aaaagcggat cgcccctgcc agcagcaggt ggaatagcgc aggcagtaat gtttccatcg
     3181 ccgcgatccc gccaagcgaa ctggcggtct ggttttccac gccaggctgg taggcgacaa
     3241 aaataaacag caggctgatg atcccggcgc tggaggccca ggccagcttg atgaaaaaca
     3301 gattgaaggc gaagttcatg ccggaagaac gtacgcgggt tttccactcg ccgtagtcat
     3361 cggcaaaggc catcaatgag aagtgcagtg gcaatgtgaa gccaagaatt acgccattac
     3421 cgaggatcac cgccagccac agcgtttgat aagccgggcc ggagggcagg aaccacatca
     3481 acaccgccag cgcagcgagg agcaggttgg tgtagtagta aattttgacg gtatcgaaac
     3541 gccgcgttaa cgggctgaca atcaccgagc cgataatgga agcgaaggtg accatggtga
     3601 agaacagcga cgtgtagccc gtgctgcctt gtaagacgta ggtaatgaag tacatatacc
     3661 caccgccgcg aatgttaaag acgttaatca gcaggaaaga catgaccagc atcagcagca
     3721 gttggtcgtt gttccgcagc ccggcaagat gttcgcgcag ggtaaatttc cccattgtgg
     3781 agagtggcac ccgttcacga acccagaaaa aacagcacag gaacatcacc acggcaatgg
     3841 cgcacagcac gccgacgccc aattgatagc cgcgcgcggc attaccctga ccgaagagat
     3901 ctaccatcca cggtaagcct actgaaacca gaaatcccgc tacaccacag agaacgaatc
     3961 gccaggactg gcaggagatc acttcattgt ggcgggtggt catggtgttg atcagcgcgc
     4021 agtacggtac gttgatggcg gtataaccga ctgaaaggag caggtaagta ccgaacgccc
     4081 aggcgatttt tactcccatg ctggcatctg gcacggtgaa cgtcaggatg ccgataatgc
     4141 cgatgggaat ggcaatccat agctgccacg gacgaaagcg cccccagcga ctttgcgtcc
     4201 ggtcggcaat gacgcccatc accgggtcgg agatggcatc aaacacgcgc agtgcaataa
     4261 acagcgtgcc aaccagagcc ggagttaatc caaaaacgtc ggtgtagaaa aaggtgagaa
     4321 aattcatgat caggcaagtt attacggtgc cgcctgcgtc acccaggccg tagcctattt
     4381 tttcccgtac agataacgca tcgtcaattt tacctttagc gatatcagcg gtagtaatcg
     4441 gtgtagaggt catagatatt cctcgtgaat agggagcaat ttttagtttt attgaaatga
     4501 cctgattaat tctgccgtga acgggaatat caacaaggcg agaaaagagt aaaaaaaggt
     4561 gatgattccg acctgaattt aaaagtg
//
LOCUS       DAPGWD010000236         4578 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPGWD010000236 DAPGWD010000000
VERSION     DAPGWD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.238"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..335)
                     /gene="umuD"
                     /locus_tag="RHX88_004253"
     CDS             complement(<1..335)
                     /gene="umuD"
                     /locus_tag="RHX88_004253"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV2232858.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPAL"
     gene            complement(332..580)
                     /locus_tag="RHX88_004254"
     CDS             complement(332..580)
                     /locus_tag="RHX88_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HDV2232859.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            998..1900
                     /locus_tag="RHX88_004255"
     CDS             998..1900
                     /locus_tag="RHX88_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDV2232860.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGSEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNCIQVDF
                     DTPWCQPESDVVAALSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            1897..2208
                     /locus_tag="RHX88_004256"
     CDS             1897..2208
                     /locus_tag="RHX88_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232861.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRT
                     R"
     gene            2285..2968
                     /locus_tag="RHX88_004257"
     CDS             2285..2968
                     /locus_tag="RHX88_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDV2232862.1"
                     /translation="MSRFVLGNCIDVMARIPDNGIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYVLAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            2969..3190
                     /locus_tag="RHX88_004258"
     CDS             2969..3190
                     /locus_tag="RHX88_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232863.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            3202..3636
                     /locus_tag="RHX88_004259"
     CDS             3202..3636
                     /locus_tag="RHX88_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDV2232864.1"
                     /translation="MYGTCEILCRELAAKYPADTPLMLVVWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRIESGISSGVAMEIISNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVINQARTLLKNNTHENN"
     gene            3687..4457
                     /locus_tag="RHX88_004260"
     CDS             3687..4457
                     /locus_tag="RHX88_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232865.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
BASE COUNT          954 a         1236 c         1369 g         1019 t
ORIGIN      
        1 tccagtgccg ggcgtgggcg caacagcagt cgtttgacgg tgaactcacc gtcgatctca
       61 gcgataacga tgtccccgtg ttgtggtttt tcggccctgt ccactaccag cagatcacca
      121 ttctgcacgc cagcctggtt catcgattca ccgctggcgc gcagaaagaa ggtggctgca
      181 ggtctgctga tgcaatagct gttcagatcc agttcctgct cagcataatc agtggcaggc
      241 gaaggaaaac cggcctggca acgatcggca aacaacggtc gcacgtaact gtcatcgcct
      301 gaagggtcag ccggacggtg ataaacggta ctcatgctgc aggcagccag ctgtcgtcct
      361 gccagatttc ttccagcaga gacaggatac gttctttatc cttgtcgttg cgggctcctg
      421 tgacagatac agacggggcg ctgccctggt ctatacgcag ccagatttcc gggtagtgtg
      481 gtttcagacg ttgttcgatt tcattctgta gcgcctgaag tgtgcctgat ttgaggtttt
      541 ttgtgctctg gcggtcgaaa agaatttcaa tgcggatcat gttctcatct cccttttctt
      601 ttgctgtatg tatatacagt attctaataa ctgaatccgc acacagtcaa atgtcattgt
      661 tgtgactaaa aaacgctcaa ttctctgagt gtgtggctgt atgcctgctg tgggctgctg
      721 tgtccagctt atccacagca ttttgtgcac ggttctgtgg acaaaatacc tggttaccca
      781 ggccgtgccg gcacgttaac cgggctgcat ccgatgcaag tgtgtcgctg tcgccggcct
      841 cctcacccgg tcacgtttcg tcgttcctcc tccacgcgct gcggcttcgg ggccgcacct
      901 gcattcgtat gcggtcgccc ggtaacaggt gcggcacggc ctgatggagg ccgcatgtga
      961 gaggagaatt cccatgccaa actggtgctc gaatcgtatg tatttttctg gtgaaccggc
     1021 acagatcgct gagattaaac gactggccag cggtgcagtt acaccgcttt atcgccgcgc
     1081 cacaaatgaa ggtattcagc tgtttctggc cggaagtgcc ggacttctgc agatcactga
     1141 aaatatccgc tctgaacagt gccccggagt gacggctgcc ggacgtggtg ctgtatcgac
     1201 ggaaaatatc gcgttcaccc gctggctgac acacctgcag aacggtgtac tgctggatga
     1261 gcaaaactgc ctgatgctgc atgaactctg gctgcagagt ggtactggac agcgtcgctg
     1321 ggaaggatta ccggatgatg tcagggaaac catcactgtg catttcaccg caaaaagagg
     1381 tgactggtgt gacatctggg gtagcgagga tgtatcggtg tggtggaacc gtctgtgtga
     1441 caacgtagtg ccggaaaaaa ccatgccgtt tgacctgctg acggttctgc cgacccgcct
     1501 ggatgttgaa gtgaatggct ttaatggtgg tgttctgaac ggtgttcctt ctgcatatca
     1561 ctggtataca gaacggtatg gcgtgaagtg gccttgtggg tatgacctga atatcagtag
     1621 tcagggagac aactgcattc aggtggattt cgacacgccg tggtgtcagc cggaaagcga
     1681 cgttgttgca gcattaagcc gccgtttcgg ctgcacgctg gagcactggt acgccgaaca
     1741 gggctgcaat ttctgtggct ggcagttgta tgagcgcgga gagctcgttg atgtgctctg
     1801 gggggaactg gaatggtctt ccccgacaga tgacgatgag ctgccggaag tcaccggacc
     1861 tgcgtggata gtcgacaagg tggcgcatta cgggggatga agtatgacgg agacgggcgg
     1921 gcaaccgccc gtttcttttc cgacaaagga tgtcgccgtg ctcctctgtt tactgcgccg
     1981 gctgatgcgg cgcggcataa acgctgcctg tggtcagtgt cccgcgtccg gcgggcacgg
     2041 gacgggcgct tttaccgctc ccggctggcg tcggtgacag tgtacgccag cctgtcgccc
     2101 ttttctgatg aacgcccttc aagccgcttt cgcggcatat tctcgccgtc aaaaagacga
     2161 cggctgcgat attccacggt cggcctgacc cgttaccgga cgcggtgaac agcccacagg
     2221 cagcggggaa cgggcaccac aggggtgccc tcccggtgcc ctctgagaaa gaaggagtgt
     2281 tgttatgtcc cgttttgtcc tcggtaactg cattgatgtt atggcgcgta ttcctgataa
     2341 cggcattgat ttcatactga ctgacccgcc atacctcgtc ggttttcgtg accgacaggg
     2401 gcgtaccatc gccggcgata aaaccgatga atggctgcaa ccagcctgta atgaaatgta
     2461 tcgcgtactc aaaaaagatg cgctgatggt cagcttctac ggctggaacc gcgtcgatcg
     2521 ctttatggcc gcctggaaaa atgcgggatt cagtgtggtt gggcacctgg tcttcaccaa
     2581 aaactacaca tcgaaggccg catatgtggg ttatcgccac gaatgcgcct acgttctggc
     2641 aaaaggccgt ccggcgctgc ctcagaagcc attacccgac gtgctgggct ggaaatattc
     2701 aggcaatcgt catcacccga cggaaaagcc tgttaccagc ctgcaaccac tgattgagag
     2761 cttcacacat ccgaacgcga ttgtgctgga cccgtttgca ggcagcggct caacctgcgt
     2821 ggctgccctt cagtccggac gccggtatat cggtatcgag ctgcttgagc agtatcaccg
     2881 tgccggacag caacgtctgg ctgccgtaca gcgggccatg cagcaggggg ccgcaaatga
     2941 tgactggttt atgccggagg ctgcgtaaat gaactatgcc ggacatgaaa aacttcgcgc
     3001 cgaagtggcg gaggtggcca atgccatgtg cgacctgcgt acaaccatga atgagatgga
     3061 gcggcggtac agctttaatg ctgacaccct gccggaacgt ctggtgcgtc agacgttgtt
     3121 tcgcgcaaac cgcctcctga tggaggcata taccgaaatt ctggaactgg attcctgctt
     3181 caaagattga ggggagagag tatgtacgga acatgcgaaa tactctgccg ggagctggca
     3241 gcaaagtatc cggcagacac gccactgatg ctggttgtct ggtcccctga agagattcag
     3301 gccctcgctg acggaatgga tatttccctg tccgatcatg aaatcaggac cgtcctggcg
     3361 cgcctggagg acatcccgga agaccagcgg attgagtccg gtatttcttc cggcgtggcg
     3421 atggagatta tcagcaacgt gagagaaaac cgccaggtga ccgtccctgc tgaactgctg
     3481 gcgtccctga ttcagaccgc tgaacaggca ctgtggaaac gtgaatgggc cgcccgtgat
     3541 aacggccttg ccgtcccgga atgtgttacc cgccgtcagg cagtgattaa tcaggcccgc
     3601 accctgctga aaaacaacac acacgaaaac aactgatgtt atcgccgcct gccctgcggg
     3661 cggcgataca gggagaacgc ttaatcatga acaaaacgct gaatgcactg gtttgtcgtc
     3721 acgcccgtaa cctgctgctg gcgcagggct ggccggaaga gacggatgtt gaccagcgaa
     3781 atccgaacta tccgggctgg atcagcattt acgtgcggct ggatgcgccc cggctggcga
     3841 cgttacttat caaccgtcac ggcggcgtac tgccgccgct cctggcctcc gccattcaga
     3901 gactgaccgg aaccggggcg gaactggtac tgtccggcag tcagtggcag tcgctgccgg
     3961 tacttccggc agacggaacg caggtgtctt tcccgtatgc cggagaatgg ctgaccgaag
     4021 acgaaatcag ggctgttctt gatgcggtgc acgatgcggt acgaagcatc tgttaccagg
     4081 tggcagaaga tgcgcggcgt atccgtgcgg cgctgaccac caccggtcag acgttgctga
     4141 cccgccagac gcgccgcttt cgcctggtcg tgaaggaaag cgatcacccc tgctggctcg
     4201 atgaagatga cgaaaacctg cccgtggtgc tcgatgccat cgtgaaccgg ggagcacgtt
     4261 tttcgtcggt ggaaatgtac ctggtcagcg attgtattga gcatatcctg tccagtgggc
     4321 tggcctgcga tgtgctgcgt ataccggatg aaccgccccg ccgctggttt gaccgtggtg
     4381 ttctgcggga ggtggtccgg gaagcccggg ccgaaatccg cagcatggcg gatgccctgg
     4441 caaaaatccg gaaatgatga cactggcggg gtaaatcccc gcctttttcc tgccggtccc
     4501 cggacagagc acaaaggaag tcgccgccgg ctccgcttta cccggccatg cagggcatgg
     4561 ccttgtggtt tttcagtt
//
LOCUS       DAPGWD010000237         4470 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPGWD010000237 DAPGWD010000000
VERSION     DAPGWD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.239"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            346..609
                     /locus_tag="RHX88_004261"
     CDS             346..609
                     /locus_tag="RHX88_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDV2232866.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            527..814
                     /locus_tag="RHX88_004262"
     CDS             527..814
                     /locus_tag="RHX88_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HDV2232867.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            1256..1789
                     /locus_tag="RHX88_004263"
     CDS             1256..1789
                     /locus_tag="RHX88_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HDV2232868.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            2043..2726
                     /locus_tag="RHX88_004264"
     CDS             2043..2726
                     /locus_tag="RHX88_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HDV2232869.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLEAAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            2740..3306
                     /locus_tag="RHX88_004265"
     CDS             2740..3306
                     /locus_tag="RHX88_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HDV2232870.1"
                     /translation="MKLQYRIPLAIYSVWCIIAILLCKDGLYQMNIWTLVEILGMIAL
                     PLLVRPFFILLRIIFRKNSFKTKDMSDNHTVCVFLSALADTSSKKAIKKHFKQLLEKL
                     PPLLRQKKRIYMKSHLLTEARTQKLICSLRRKGLDVTAERRESAMNSVMFRILIVSRI
                     LSQWKVPHINPRCGIVILTLKNDSGNRE"
     gene            3574..3828
                     /locus_tag="RHX88_004266"
     CDS             3574..3828
                     /locus_tag="RHX88_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HDV2232871.1"
                     /translation="MPQQHPGRLQVLVVDTHCKRKLFSTKTQTDPDELARRFCTPDNC
                     LVVVLCNNRFLFRLERAPGSHCRWRKGSRSRHQHLQDWLS"
     gene            3871..4323
                     /locus_tag="RHX88_004267"
     CDS             3871..4323
                     /locus_tag="RHX88_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HDV2232872.1"
                     /translation="MSFLFKLTIAILIVFAILFPILISSDNKLSALFWKIPKDFWVFL
                     IIMYGAIITVVFIESSEQARIRRQQYLQQYKMPNEIHENVVIENSIISRETTDDESDI
                     NTLYLDSFKSKNNCKSFETNSNLLDKNGKPVEIMVCDVQELHFFVPKN"
BASE COUNT         1237 a         1074 c          972 g         1187 t
ORIGIN      
        1 accatttgtt acacaacccg ccaattgtgt aacaacagcc tctacaagag catatttgtt
       61 catatgttcg tcagaactgc ggattgtctg gacgagggtc agacaccgtt tcgtgcatat
      121 ggaccagcat gttcgaacgc cacaaagcag aagcatcatg cctggatcgc ttcggcgcgg
      181 agcgttaagt tttgacgctt ttttgtatgt tttttaagct gttagttgac tcggtgcgtt
      241 atactaagta ccctttactg gtcacaggca aaactgtgat cgggcgtagg aacccgcgta
      301 gtcatcgtcg gcgcagaagt taatttcttc atttggcgtc gttttttgcc ataccataaa
      361 gacaggaact tgtatttctt gtcgtcatct atggtagctc aggcggggca ggcattagcc
      421 tggccggttt ccgatgacgc cggtattcct acccccgtct gggctatcac caatgagcgt
      481 aggaactcgg tgatagcgtt tttagttgtc atcggagacc tttgctatgt cacctcaact
      541 gatacagaaa ctcccggcca tcactctgtt ggaaggcatg tttcccgagc tatcaacaaa
      601 ccagcttaaa gtctgcgttt tttacgcaat gggtgttcct tacgacgcca ttgctcagaa
      661 ctgtcggctt tctcccgaaa cggtacgaac ctacctgaag cgaagcctga agaatttaaa
      721 tctggaagga tatgatgcac ttcgctctgc tgtgctgatg cgaactttcg ttttcatgat
      781 tagcaataca gctaaagaaa acgaaaaaat gtgaacaggt gtccccatta aaggggacac
      841 tttcatggaa attgcatttt gcactttaca ggcgatccca aattgataaa cataaacacc
      901 aagcacaagt tactttaata aagcaaatca cttcagacgg tgtcccagtt atttgtatct
      961 ggctcatgcc cgatatatgg cgaccttact ctgaactgac gtgctgatgt tttatttcgc
     1021 gtatcacaat actgtgccac tgtattgctg atattagtgc ttagccgttc tttatctgtg
     1081 agttcgccaa atcaccgata cagaacgtac tagagtctgc tggttatatg cctgtcagaa
     1141 ctgcagttta tccagatatg ggctggacac tgcttcagac atatgattta gcatattcgc
     1201 ctgacatcgc aaaatcgtcc atcatggatc ggttttgcgc ggagcgttga acttcgtgaa
     1261 tatagaacac ctgaataacc ggaactggta tcttgcgcaa tataatacag ccggcaaaaa
     1321 cagagaatct ttattctcat ggctgaatga gcaaaacgtc gtaccatgga ctccattaat
     1381 aacaagaaag ataagacggg cagactccag gtgctgttac cgtgaaagaa tattcgctat
     1441 ttttccaggg tactttttta ttctggctaa ttttgacatt cacccagtat cagcattacg
     1501 tcgacatagc gccttcattg atttcgtgaa gtttggcggt gaaataaaac ccgtcaataa
     1561 agatattgtt gatggattaa tgaagattta cccggaccct gtgctaaatc cgggagccag
     1621 ggaagagctg aatgcggctt ccagcatatg gctgacaaaa gcacaatatc agtatctcct
     1681 cagaatggaa aatactctcc agcctgagtc gaggatatcc ctgctcctgg aactggtatc
     1741 aaatgccgaa catcacggct ttatggagcg ccttgtaaac atcccatagc cagtatttcc
     1801 cggccttcgg gccgggcttt tttcaattca agagaatatt ccggagtgaa gcctgcacga
     1861 agccatcccc accatggact ccccctgtct tttgatggaa ccgtgtattt cttaatggcg
     1921 aaagaaataa aaggcttcac cccagagtat tgattcgttt tatcaaaggc cggcccgtgc
     1981 catcacgtgt ggcggagaga acggataccg gtcttctttt catctcacag taggacattc
     2041 gcatgaccac gacagaacat cccgtgcgcc agttgctctg gtgtgcactg aatgccctga
     2101 aaacggcaca ggaaaaccac aatataacca gtgaaaccgg attacggcat tacctgcttg
     2161 aatggctttc tggtgccagc agacatcctg agttccgtag tctccctggc gaaatcatgg
     2221 cgttaaaagc attgaccgaa aaagatcgca atattcccat catcggaacg ctgaatacgc
     2281 tctttctcag ctctgccact gtcggggaat gtccgttgtt tcgttttcgg gctgcactgg
     2341 gtaaattaca gaaagcaggc tggcgcacat atgtctgccc atggcctgag agggtgttca
     2401 atgaatccat tgaacgggcc tgttccggga aacgtcatct gctacaacta agtcgcaccg
     2461 aagagtgttt tctaccgaca ggcgatatga gggcaccagt tacgttgcag ctgatcactc
     2521 cactacaccg caaaactatg cagcttctgg aagcggcaga gttaatattc tccgacgaag
     2581 gatttcaggt ggtgagagga tacgaacatc aattcgggat cgaacgcaga gaaaccctct
     2641 ttcacacctt atttatcggg ctgcccagcc tgccagaaga tgtctggggt gcaacacagg
     2701 agcagacgac tatcacatta cactgaggaa gagcaataca tgaaactcca atatcgtatt
     2761 ccattagcta tatatagcgt ctggtgcatt atagcgattt tgctctgcaa ggatggcttg
     2821 taccagatga acatatggac gctagtagag atattgggga tgatagctct cccgctgtta
     2881 gtccgtccgt ttttcattct gcttagaata atatttagga aaaatagttt taaaaccaaa
     2941 gacatgtctg acaatcacac agtatgtgtt ttcctgagcg cactcgcaga tacttcctca
     3001 aagaaggcga taaaaaaaca cttcaagcaa ttactcgaaa agctgccccc cttattacgc
     3061 cagaagaaac gtatctacat gaaatcccat cttctgactg aagccaggac acaaaagctg
     3121 atttgctctc tgcgccgaaa agggctggat gtaacagcgg aacgaaggga atccgccatg
     3181 aatagcgtca tgttccgcat cctcatcgtc agcagaatct tatctcagtg gaaagtgcct
     3241 cacataaatc cccgctgtgg tattgtcatt ctgaccctga aaaacgattc tggaaacagg
     3301 gaataacagt tataagagca ccacgatttc cgacaggaaa acatacatga aaatcaacgt
     3361 agatgaccct ggcaaaaaga gcttcgctct cattacgcct cacgtgcgtt aattcccgac
     3421 acaccccgtg acccgtcttc gcagcctgta ccaccctttc tgccgttccc gggattcttt
     3481 ctgtcactac tcgcttgtgc cgttttttcg caaccggtac gtgcagttac gacggtacag
     3541 ctgcttcttt tcccgttccg aaaggagtct ttgatgccac agcaacatcc cggacgacta
     3601 caggtactgg tcgtcgacac gcactgcaaa cgcaagctgt tttcaacgaa aacacagaca
     3661 gatcccgatg agcttgcccg acgcttctgc acgccggata actgccttgt ggtggtacta
     3721 tgcaacaacc gatttctgtt ccgcctggaa cgtgcacccg gtagccactg ccgctggcgc
     3781 aagggtagcc gctcccgcca tcagcacctg caggactggc tgtcgtaaaa acaacaaaaa
     3841 caaaagacct cgtcaaaaca ggaggggacc atgtcgtttt tatttaaatt aaccatcgcg
     3901 attttgattg tctttgcaat cctttttccg attttaattt catcagataa caaactgtcc
     3961 gcactttttt ggaagatacc aaaagatttc tgggttttcc tgattatcat gtacggggca
     4021 atcatcacgg tggtgttcat agaaagcagt gaacaggcac gcatccgccg ccaacaatat
     4081 ttgcaacagt acaaaatgcc aaatgagata catgaaaatg tggtgataga aaacagtatt
     4141 atttcaagag aaacaacaga tgacgaatct gacattaaca ccctgtacct tgattcattt
     4201 aaaagtaaaa acaactgcaa atcttttgaa accaattcaa atttactgga taaaaatggt
     4261 aagcctgtgg aaataatggt gtgtgatgtt caggaactgc attttttcgt tccaaagaat
     4321 taaacagtta atccccctta ctctgttatc ttagtcgccg gcatcaacta acatgaaaaa
     4381 taataacgta ccattatatc tatggttact ttggtgtacg ttttgcctgt acatcactac
     4441 cccgtggcaa tacttccgtt atctatacac
//
LOCUS       DAPGWD010000238         4386 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPGWD010000238 DAPGWD010000000
VERSION     DAPGWD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.240"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2684)
                     /gene="traG"
                     /locus_tag="RHX88_004268"
     CDS             complement(<1..2684)
                     /gene="traG"
                     /locus_tag="RHX88_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HDV2232873.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTWTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLILAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTNASLGLY
                     GDAYAKGHLGISVLGNGGGVGLQAGAKASIDGSDLDSHEASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKNAPQDVEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNTWRESRRDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVKGQYSELQ"
     gene            complement(2681..4054)
                     /locus_tag="RHX88_004269"
     CDS             complement(2681..4054)
                     /locus_tag="RHX88_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HDV2232874.1"
                     /translation="MPRIKSLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRMQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIGHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(4054..>4386)
                     /gene="trbF"
                     /locus_tag="RHX88_004270"
     CDS             complement(4054..>4386)
                     /gene="trbF"
                     /locus_tag="RHX88_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HDV2232875.1"
                     /translation="RDYKYISRITGRYAGLSLVFLTAGIVLWTVMDIIFDACIDSWKA
                     DPELNNSSYMWNTLIYAIPYTLYALAAGFLVTFFSVPNVRINIRKYRDIPAEMSYAPG
                     EHIKGGQE"
BASE COUNT         1047 a         1185 c         1030 g         1124 t
ORIGIN      
        1 ttttgtagtt cagaatactg cccttttaca atattttcct cgcctcggat gctgttctga
       61 gtatctccga tattacctcg atattctgtg accatattat caacctgatg ttttacatca
      121 ttacggatat tactgtcctg cgttctttgc tcaataatgg cctgatgtcc ctgatgatca
      181 gcaataatat cctggctgcc accacccgaa ggtacgctct ccattccttt acctatatct
      241 ctacgggatt cacgccaggt gttatcaaca ccaggctgta cctgttcctg cacaaaagac
      301 cacgccatag cccgacggcg ttctgcaatc tccggcgaac tggtatttgt cagaatagct
      361 tcaacatcct gcggtgcgtt tttcatcaca tactgtgcaa actgttgcga cagatcttca
      421 ctcatctgcc cactcatact ctcagtacgg gatgccattt cagcatattc atggctgcgg
      481 gtcatattcg tcgtgtactg gtcgtaactc tgtttcgctg aattcagggc tgcagacaac
      541 tgatccacac gggaatcagc attattgtcc gtatggctgc cggattcact gactttgcgg
      601 ctggtaaagt aatcgctggc ttctttaaag tcttgtgtag ctctggcatc gatatcatga
      661 cgagcatcat ggctggcccg tgaaccacta ctggcttcat gtgagtcaag atcactgcca
      721 tcaatgcttg ccttagctcc ggcctggaga ccaacccctc caccgttacc caatacactt
      781 atgcctaaat gccctttagc ataagcatca ccgtaaaggc caagcgacgc atttgtactt
      841 ctcgaggcca gttctctcgt cgcctgctca ttactgatat tatgcgcctt cgcatagctt
      901 tccactgcac tgcgcatacg gctggccatc atggagtcct gagcactcat cgtgctgtca
      961 gcaccaccag tgacagagtc actgctcccc cggttagagc caaactgact gagtgtgttc
     1021 caggcactgg caatactgct gctgaaacca tgcagggcac tttcggctct gttcgacgcc
     1081 tcccgggcca tttcctgttg tgccgctgca atctgacgcg ttgcattgat accgaccggt
     1141 aaccgggaca tcgctccgct tgcatccatc accatattac cgtcacgggt ctgtgtggcg
     1201 gttgcgccac tgccggtctg gtacatcatc tgaccaaacg acgtggtgct gttggtgctc
     1261 cagctgaagc cgttcacgtt ttccgtctgc atgttgccgt aggagtaatt accgtcaacc
     1321 acactgcctg ctgcactggc cgttggactg atagatgaag aggcgaagtg gctgtacacg
     1381 ctggaaaaac ctgcgccaag gccttttacc atcatccatg acaacggggg gatcatggct
     1441 gaaagatacc cggcagtgga ggccaggtca gagtatttca gctgtatctg agagagttca
     1501 gacaacacga ccggcgcacc attctgtttc gcatagaatg tcatggcact gttcagaatg
     1561 gcatacaaca acggccaggt ctgcagccac atcagggcaa acacataccc ctttaataca
     1621 gacaatgtca gcttgttaaa gacagccgcc agtatcagga gcgggaatat accaatggca
     1681 atccccgtca ggatagtctg gaccatcggc aggtttcgca tagttacatg accaatggag
     1741 acatgtgcca gacgttgttt ctccatcgat gacgtggtgg ccagacttac caggctggcg
     1801 gtgtcaccgt tacgggctgc attactggtg ataccctctt tcagggcatt catggtgacg
     1861 ttctggcgca taatctggct ggcagactga ctggagccgt agaaataact gtaactgtca
     1921 ctgaccagtt gtctgaataa caggtccggg tccggtctgc cgccaaatat ctgctgcaca
     1981 taataatgcc aggtcttgcc tcccgtcttt gtatcgagat tcagcctgtc tttcagcgtg
     2041 accgacgcat ccttacaagt tatgaaatta ttattgttgt cataaacgcc ccgtagcggg
     2101 ctggggcggg aaaaaatcag cgtgtagggg tcagcggagg ccatcaggtc ttccagcgtg
     2161 tatttatgat tcaggtaaat atcacccagc acgcagttct ggacgtagtc ctggaacaga
     2221 ttgatgattt ccggattccg ggacaggaaa tcggtgcttt tcacaatcag attcgccccg
     2281 aacagcatcc ccgttttgct gtaggtgacg ctgtccggtt gcgtgaagat catctcgtaa
     2341 ctggccacca ttgcatgccc gatacgggtc gtcagtgaaa gtggcatcgc cagaccgacc
     2401 ggcacattat ccacccggtg aactttgacc aggtcactgt tatcaataat ctgcaccgat
     2461 gtgcggacat taaccagcag gctgataagc acaaacacgg ccacccagcc cagcaaatcc
     2521 atcacgttgt gccgctgtac ccacattacg gccaccgcga gaacagacaa tgtgagcgca
     2581 attttttcaa tggaatccca cgtccaggtt cccataaagg cggcaatggc gttcaggtta
     2641 ttccgcaacc actcaccacc ggcaatcaca taaacttcat tcacagcgtg ctccctccga
     2701 agtgatagtt gttctggtaa cgactgagca tgcgggcgga aagctgctga cgcatgtagc
     2761 tcatctgacg atcgacaacc agcagcgcat cctgctgtac ctgcacctgt gactgaaacg
     2821 ccgcaatctg ccgggtggca tcattcatgt tgtcgttaat atgcccgata accgcttcgt
     2881 cataatttcc cgtggccacc atcgcccgtg cctgctgtat cagctcctga atgtactgca
     2941 gcaggatgtc gtaaccgata tagtccgtca gctggtaaat catactgttg gaaacaccga
     3001 gcatctgcgg gtcaaccagg tatttgaaga cggggatggt ggtactggaa ataaagcctt
     3061 tttccttgtc atccagaggc gtgtcactga cagccttgtt ctgaatgctg gccagaagtt
     3121 tagtaatctg agacttcagt gcattatccc ggctgatggt caccggtgtg tctgccacca
     3181 ctttcaggca tttatcagaa tcgttgcagt gataaatttt tgctgtgccg ccttccatca
     3241 tggcccggat aattgagcgg tcagtggttc tggctgacag cggtgtgatt tcgccgttgg
     3301 gaccaaacac cagtgagccg gtcagcgtca tcacaaactc tttcagctct ttgttgccgt
     3361 caaacattct gtttttggaa agcgcattcc acatgatgtt aatgtttttg gtcacacgct
     3421 ccttatcctt gtcgctggct ttatccctga cgctgtcaga tttaccgcca acggtacatc
     3481 cctgccggga agccgcccag tcagcaaaaa tattgctctc accggcaatg tcctgacaga
     3541 ctttttgctg ggacacctgc atccggggga aaagcccgcc gataatcccc tgcgccgcct
     3601 ggcaggaact gaggttcatg ctgttaatgt cgcttgccat tttctgcagg aagtcttttg
     3661 cggtttttat ttccggcacc gttgtctgca gggcaaggtc aaaaaagtaa ccggcagcat
     3721 tgctcataat ctgcttaaca aaacgctgca actgctcacc attaataaaa ctgaacgagc
     3781 cgagataggc gtcgatgccg ccacatccag cattgatatc cggcagcgtc atggaaatta
     3841 gctgaacatt tttgacctga gtacgggcat acagggagcc accgtaagca tagccggcgg
     3901 cctgcccctg ccagacgccc ggctgtgtgg tattggaagc aaatcccagc ttattaaaga
     3961 actgattcat gtcgctgttc acatccgctg acgctgcagg cgtcaccgtc agtaaggcgg
     4021 cacaaagaac cagaagagat ttaatgcgtg gcatcattct tgccctccct taatgtgttc
     4081 tcccggtgca tacgacattt cagcaggaat atctcgatat ttccgtatat taattcgtac
     4141 attggggaca gaaaaaaatg taacaagaaa cccggctgca agcgcatata acgtatacgg
     4201 aatggcatat atcagggtat tccacatata actcgaatta tttaattccg ggtccgcttt
     4261 ccacgaatca atacaggcat caaaaataat atccataact gtccataaaa caattccggc
     4321 agtcaggaat accagagaca acccagcata cctgccagta attctggaaa tgtatttata
     4381 atctcg
//
LOCUS       DAPGWD010000239         4353 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPGWD010000239 DAPGWD010000000
VERSION     DAPGWD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.241"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..144)
                     /locus_tag="RHX88_004271"
     CDS             complement(<1..144)
                     /locus_tag="RHX88_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDV2232876.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRA"
     gene            complement(147..866)
                     /gene="sanA"
                     /locus_tag="RHX88_004272"
     CDS             complement(147..866)
                     /gene="sanA"
                     /locus_tag="RHX88_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDV2232877.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1016..1900)
                     /gene="cdd"
                     /locus_tag="RHX88_004273"
     CDS             complement(1016..1900)
                     /gene="cdd"
                     /locus_tag="RHX88_004273"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDV2232878.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAENADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2030..2725)
                     /locus_tag="RHX88_004274"
     CDS             complement(2030..2725)
                     /locus_tag="RHX88_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDV2232879.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2722..3120)
                     /locus_tag="RHX88_004275"
     CDS             complement(2722..3120)
                     /locus_tag="RHX88_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDV2232880.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3359..4309
                     /gene="dusC"
                     /locus_tag="RHX88_004276"
     CDS             3359..4309
                     /gene="dusC"
                     /locus_tag="RHX88_004276"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDV2232881.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         1152 a         1113 c         1128 g          960 t
ORIGIN      
        1 tgctcgccag acatacaccg gttccggtgc atttttaacc cgtttatcaa tcgcgcctgc
       61 gcgccagcct ttccatgctt cgcggaaaca aaatatcgga ataaggaaaa aaacggcaac
      121 gacaaaaaac tgctgtacat ccataactac tttccttgtt tcttttgtaa ttcaagtaac
      181 tgttcgggtg tgacggcggg atacccctgc gcatcttccg gcacctggtg catagccgga
      241 atagggacca gcggccctaa aaaacgcggt tcacgtttaa aaatataaag gtcagccagc
      301 gcaccgaaac gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt cggtgacggt
      361 acggcataac actgagcctg aatccccata tgcagcgcaa taaataatgc ccgctcacag
      421 tggaaacgtt gggtgataat aatgaaatca ttagtatcga aaactttgcg tgtacgcacg
      481 atggaatcca gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg gtcgacacca
      541 gcagcgatta aatctttgcg catggtcatc ggctcattat aactttgcaa tgcgttatcg
      601 ccgctcagta atagataatt taccttaccg ctgttatagg cattaatcgc tccttgaatg
      661 cggtagcgat aatactgatt aattacgcca gtacgataat attttgctgt tccgagcacc
      721 acaccgacct ggcggtaggg gagatcctgc aattcgtcgt agatataagg cgcggttttc
      781 cagctcatcc agcgatcgag gccgagcaca gtcaacagca gcaagccgat caggactaac
      841 aggctgagga acacgcgctt taacatgcag gagactcgat ctaaggaaaa tttccatcag
      901 gctactttac ccgctggttt tgcgcaagaa acggcaaact gtttgccggg aatttcgaca
      961 ccaggagagg gctggcaagc cggacaaggc gttcacgccg catccggcac caggcttaag
     1021 cgagaagtac gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg gaggtggcat
     1081 cccactgaat caacggcgca tcggcgtttt ctgccagaac cgcgcgctgg atatccgggt
     1141 aatcataacc cttgagattc aatagaatta acgctccctg caacggtggc agagtcgggt
     1201 tgaatgcggc gttttcagcg tagctgccgc tgaaaatacg accgtcttta cattccagcg
     1261 cgacaccgct tggcgactta ctgtaaggca tgtgcgaacg gtttgccgct gcaatcgccg
     1321 cctgagaaag cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc atcagcagcg
     1381 ttttaatctc cagatctttc ggcccaaagg catccggcag atagtcacgc agcgcgtgtg
     1441 cctcgcggcc cggcagatga atacgcagat ccagaccgct gttcagttca ttcataaact
     1501 gacggcagtg accacaaggc gtgtagttaa cggtgatggc tgcaagcgct ttttcaccac
     1561 tcaaccaggc gtggctgatc gcgctttgtt cggcatgaac ggtttgctgc attgtcgcac
     1621 caataaattc catattggca ccgaaatacc aggttccgct cacaccgcgc gcaattgcgc
     1681 caacattaaa attcgacaat ggcgtacgcg cacaggccgc cgccagcgga agtagtgcga
     1741 atgccagcgc gtcttcgtcc agccccgttg cgctcttcag cgatgagact tgctccccgg
     1801 tcaacaaagc ggggaagtac ttgtctgcca gaataggttc cagtgcagat tgcaagttat
     1861 ccgcaagttg ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct cataatcatg
     1921 taatgggata acagtttacg aacccgttat ggctttatat gtgatctgaa tctcattaat
     1981 tatgcaacgc gtgaaactta catcaaaaat gcgcgacgca tcgcaaattt tagcccatta
     2041 ctgccagaat aatcgggaaa aggaacggtg cgatcagcga agtaattatc ccgcataaca
     2101 ccagcgctag cgaactaaat gcaccttcct gataatccag ctcggcgcag cgcgccgtac
     2161 cgagggcgtg cgaggcagtc cccattgcca gaccgcgcgc agctttggta cgaatacgca
     2221 tcgcattaag caatgtatgg ccaaataccg cgccgaggat gccgacgaaa atcacgcaaa
     2281 ctgcgctgat tgccggaata ccgccaatac tgccgccaac cgccattgca ataggcgtgg
     2341 tgactgattt cggcaggatt gacgcggcga tttccggtga agcgcccatc aacaatgcca
     2401 cggaagtccc cgtcaccatc gcaaccacgc tgccgataaa acagatggtg atgatcgatt
     2461 tccagcgcgc gcggatctgg tgtagctgct catataaagg ataggccagc gcgacgaccg
     2521 ccggttgcag cagatcgttt agcacttcgc tacctttaaa gtagctgtcg taagagatgc
     2581 cggtcagcat taaaaaagga atgatcacca ccattgctac cagcaacggg ttaagcaggg
     2641 gaaacttata ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac gtcagcggta
     2701 atgaccacca gatattcgcc atcattcttc tgatcctttc tgacctacga ctttacgttc
     2761 accgtgtacc agttgcgaac tccagctcac caccagaaaa accaccagcg tactgacagc
     2821 acaggaaacc actaccgggc caaactgtgc gcggagcaaa tcaaaatatt gcatgacgcc
     2881 tacgccaatc ggcacaaaca atagcgccat atagcgaatc agtacgtagc accccggatt
     2941 gacccatttt gccggaagaa tttgcaaggc cagcaggaca aacaggatca gcatcccgat
     3001 gatgctgccc ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg catacaggca
     3061 ggcataaatc aggacgaaag cgcgtaaata ttgccagata atgttcagtg tcttactcat
     3121 aatcaacacc ccagatgaga tggattcatc atacaattaa ttcgtaaaac gtgctaccga
     3181 tcacattaag aaatcagcgc ttattttttc gctccccggc attacgcaat gcgcaacgaa
     3241 ctcaggcata catcaagcat agagagggat tgcagtttgc agcaatccct ctttgtagac
     3301 ctttaatccg ctgaccatag gcgctaacat agcgcctcat tattttgcgg gtgatgatat
     3361 gcgtgtgtta ctggcaccga tggagggagt gcttgactct ctggtgcgtg aattgctgac
     3421 cgaagttaac gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg atcaactgct
     3481 gccggtaaaa gtttttcatc gcatttgccc tgagctacaa aacgccagcc ggacaccatc
     3541 cggtacgctg gtgcgcgttc agttgttagg tcagttccca caatggctgg cagagaacgc
     3601 cgcccgtgcg gtggaattag gttcctgggg cgtggatctc aattgcggct gcccgtcgaa
     3661 aacggttaac ggcagcggcg gcggggcgac gttactcaaa gatcctgaac tcatctacca
     3721 gggtgcaaaa gcgatgcgtg aagctgtacc agcgcatttg cccgtcagcg taaaagtgcg
     3781 tctgggctgg gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc aacaggctgg
     3841 cgctacggag ctggtggtgc atgggcggac aaaagagcag ggttaccgcg cggagcatat
     3901 tgactggcag gcgattggcg agattcgcca gcggctgaat attccggtga ttgccaacgg
     3961 tgaaatctgg gactggcaga gtgcgcaaca atgcatggcg atcagcggct gcgacgcagt
     4021 gatgattggt cgcggggcgc tcaatattcc caacctgagc cgggtagtaa aatataacga
     4081 accgcgaatg ccgtggccgg aggtggttgc tttgctgcaa aaatataccc gtctggaaaa
     4141 gcagggcgat accgggttat atcacgttgc gcggattaaa cagtggttga gttatttgcg
     4201 taaagaatac gatgaagcaa cggaattatt tcagcatgtt cgggtgttga ataattcccc
     4261 tgatattgca agggctattc aggcaattga tatcgataag ctgcgataat agtggaatcg
     4321 cagtttatca gagacaatga atcgcttatt cag
//
LOCUS       DAPGWD010000240         4348 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPGWD010000240 DAPGWD010000000
VERSION     DAPGWD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.242"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            117..911
                     /gene="hyxR"
                     /locus_tag="RHX88_004277"
     CDS             117..911
                     /gene="hyxR"
                     /locus_tag="RHX88_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HDV2232882.1"
                     /translation="MKKPLVIISACQFTRLALESLIPADRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPASSLYNGFP
                     HIGTLLKASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATQALRKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YAQNMVSGILYPEKVIVGKSINSDAISGKVLSGQRKHMSKKRPGVL"
     gene            1065..1826
                     /gene="hyxA"
                     /locus_tag="RHX88_004278"
     CDS             1065..1826
                     /gene="hyxA"
                     /locus_tag="RHX88_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HDV2232883.1"
                     /translation="MRFVLFCPSGIVPAQFEALSTGSVCNITCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKTGMS
                     VERAISVANASGGFSGLKHAENLPVMDSYSVFMNEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1868..2950
                     /locus_tag="RHX88_004279"
                     /pseudo
     CDS             1868..2950
                     /locus_tag="RHX88_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2972..4165
                     /locus_tag="RHX88_004280"
     CDS             2972..4165
                     /locus_tag="RHX88_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2232884.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSL
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
BASE COUNT         1211 a          939 c         1079 g         1119 t
ORIGIN      
        1 aaatcaaaca aattagattt atattccaag aaaattaaat attcaggcta ttaaaccaaa
       61 cctgacattt tgaatcaaaa tgtcaggttt tgtagcaatg gcgtggacga gtctgaatga
      121 agaagcctct ggttattatc agtgcctgtc agttcaccag gctggcactg gagtctctca
      181 tcccagcgga caggtatatc gtgcgggtgt acagcaacgt cactacagag gttgaattcg
      241 ttttaaagac ctcctgtggc tacctgttgg ctgatatccc ttcgcttcct tcgggggagt
      301 tagttcttct ggcttgtctt gtccgcttgc ggagtgatgc cgggcaatgg caagtctgcc
      361 tgaccggtga ctccgactgg ttcgataatg ctcctgcttc aagtctgtat aacggcttcc
      421 cacatatcgg caccttgctg aaagcgagtc gttatcagaa tcaaattatc cgctggttac
      481 tacgtccttt acccggcgat atgagtgatt tctggctcct gactgcacgg gagttaagcg
      541 tgcttaagat tctgatgcag ggcatgagtt tcagtgagat tgcccgttat gaacaacgca
      601 gttcaaagac tctgcatgcg attgccacac aggcattaag gaaacttgga ctgggaacac
      661 taagcgattt caggctgctc tatacaggat gcggtgacag cagagtcaaa cggaatatac
      721 ggctacatgc aagatatgtc ggacaacatt ccgctcatcg actcactcag tatgcgcaga
      781 acatggtgtc cggaattctt tatccagaga aagttatcgt tggtaaatca ataaattctg
      841 acgccatatc cggaaaagtg ctttccgggc aaagaaaaca tatgtctaaa aaaagaccag
      901 gagtgttatg acacagcagc gttgttacta ccacccactg cggcgcgtac ggactcgcta
      961 cagtgtaggg gccaaaatat ttctactgct ctgggctgca ggagtgggag gcacgctgta
     1021 cagcctgtac agcgtgctgg cgtactgaca tctgtgaggg aaggatgcgt ttcgttcttt
     1081 tctgtccctc aggaattgta cctgcacagt ttgaagctct atcaaccggt tctgtctgca
     1141 atattacctg catcagaaca gaagaagagt tacgcaataa attgcggcac aggccgcaga
     1201 gtgtcgtgat atcggcaggg cgacctgccg aatgcgctga aatgtggttc cgattttatc
     1261 gggatcactc attcgtggtg gtgttgtgcg tcgcgccctt ttttctgcca ccggatgtga
     1321 gtatttctgg cgttctcaaa aacctgaggt tactgaaaac aggaatgtca gtagagcgtg
     1381 ctatcagtgt tgcaaatgcc tctggtgggt tttcaggact gaaacatgca gaaaatttgc
     1441 cagttatgga cagttactct gtctttatga atgaggtgaa taaccgcaca aagacaatcg
     1501 tcatgtcaga acgcttcccg gaaaagcaaa agaaggtgtt gtcgttgctt cttgctggac
     1561 acagctggga atacagtgcg cagtttctca aaacggggat aagacagata tggcttgctg
     1621 agcagtcgct aaaaaagcgt tggggaatac cggacagcat gtcattgcgg gaagccctgc
     1681 tgctgagcag caataaattt cacggtgatg gaaatgcatt ggaaaaaacg aatgccatga
     1741 cattgcgtga aaacggaaat accaacaggt attccgtggt ggtaaatgca gggacgcagg
     1801 ctttatctct gcataaatat aaataagtgg gaaattgaaa ttagttacca gaatggagaa
     1861 tttcttcatg aaaaacagta aggcatttta ccgcagcgca ttagcgacag ctattgttat
     1921 ggctctttct gcaccagcat tcgctgctga taacgcggta tcaactacac cggttacact
     1981 ggataaagat aagacaactc tggatcaaga tgttgttatt agcaatacag cagaccaaca
     2041 gattacagcc gtaacaatta atgcggcaga taaagatctt aatgttactt ttggcggtca
     2101 tgatattact gctgaatcaa cggcagacaa aaaattcctt gaaggtgtaa aagttagcgg
     2161 tgacaaaaat gttgtgatta atgctacagg ctccaccatc acagctcaag gtgaaggcac
     2221 ctatgtccgg actgcaatgg tcatttcttc aactggcgat gttgttgtta atggcggtaa
     2281 tttcgttgca aaaaatgaaa aaagtagtgc gacaggaata tctctggaag gggccacggg
     2341 aaataatgta acgctaaatg gtacaaccat aaatgctcaa ggtaataaga gttccagcaa
     2401 cggctctacg gcaatttttg ctcaaaaggg tagcgtattg aatggtttta aaggtgatgc
     2461 aaccgacaac attacccttg ctggctcaaa tattattaat ggccggattg aaacaatagt
     2521 tattgccaag gagaatacgg gaactcatac agtcaatctg aatattaagg atggctcagt
     2581 aattggggcg gctaataata aacaaacaat ttatgcttct gcttcggcac aaggagcagg
     2641 ttcagcaacg caaaatttaa atttatctgt cgccgattca accatctact ctgatatcca
     2701 tgccctttct gcaagcgaga attcagccgg taccacaaca aatgtaaaca tgaacgttgc
     2761 ccgctcttac tgggaaggta atgcttatac cttcaatagc ggcgataaag cgggtagtaa
     2821 tctggatata aatctttccg atagctcagt ctggaaaggc aaagtttcag gggcaggaga
     2881 tgccagtgta tctctgcaaa acgggtctgt ctggaatgtt acgggatcct caactgttga
     2941 tgctctggca gtaaaagaca gtacggttaa tatcacgaag gctacagtca atactggcac
     3001 gtttgcttct cagaacggca ctctgattgt tgatgcctct tctgaaaaca ctctggatat
     3061 cagcggaaaa gcgagcggtg acttgcgtgt ttacagtgcg ggttcattgg atcttatcaa
     3121 tgaacaaacg gcatttattt ctaccggcaa agacagcact ctaaaagcca caggcacaac
     3181 ggaaggtggt ctgtatcaat atgacctgac acagggagct gatggtaact tttatttcgt
     3241 aaaaaacacg cataaagcat ccaacgccag ctccctgatt caggcaatgg cagcagctcc
     3301 ggctaacgtt gctaatctgc aggctgacac gctctcagcc cgtcaggatg ctgtccgtct
     3361 gagcgaaaat gacaagggcg gcgtatggat tcagtacttt ggcggtaaac agaaacatac
     3421 caccgcggga aatgcatcct atgacctgga tgtaaacggt gtaatgctgg gtggtgatac
     3481 ccgcttcatg actgaagatg gtagctggct ggcaggtgta gcgatgtctt ctgcgaaagg
     3541 tgacatgacc accatgcaga gcaaaggcga cactgaaggt tacagcttcc acgcttacct
     3601 gagccgccag tataacaacg gtatcttcat tgatactgct gcacagtttg gtcactacag
     3661 caacacggca gatgttcgcc tgatgaatgg tggcggtacc atcaaagctg actttaacac
     3721 caatggtttc ggtgcgatgg ttaaaggcgg ttacacatgg aaagacggta atggcctgtt
     3781 tattcagcca tatgccaaac tgtctgcgct gacgctggaa ggtgtggatt atcagctcaa
     3841 cggcgtggac gttcattctg acagctataa ctctgtgctg ggtgaggccg gtacgcgcgt
     3901 gggttatgac ttcgctgtgg gcaactcgac cgttaaacct tatctgaatc tggccgcact
     3961 gaacgaattc tctgatggca acaaagtccg tctgggtgat gagtctgtca atgccagcat
     4021 tgacggtgca gcattccgcg tgggtgcagg tgtacaggct gatatcacca aaaacatggg
     4081 agcatatgca agccttgact acaccaaagg tgacgacatt gagaacccgc tacagggtgt
     4141 agttggtatc aatgtgacct ggtaagaata aagcagtcgg ttcccctgtc gactgttata
     4201 ccccctgcag gcagcatact gctgcctgca ggctataaac atccagtatt catgatgctg
     4261 gttttttgct gatatttacc ccgaacattc ctgatacagg ctattaatac aaccttatct
     4321 gcggcagatt ttattttctg gatatatt
//
LOCUS       DAPGWD010000241         4303 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPGWD010000241 DAPGWD010000000
VERSION     DAPGWD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.243"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            359..1261
                     /locus_tag="RHX88_004281"
     CDS             359..1261
                     /locus_tag="RHX88_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDV2232885.1"
                     /translation="MYFSAEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLSAAGRGVVSTENIAFTRWLTLLQDSVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDISVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLDHWFAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            1258..1569
                     /locus_tag="RHX88_004282"
     CDS             1258..1569
                     /locus_tag="RHX88_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232886.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RWVRDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            1646..2329
                     /locus_tag="RHX88_004283"
     CDS             1646..2329
                     /locus_tag="RHX88_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDV2232887.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSRRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     NNWFEPEAA"
     gene            2330..2551
                     /locus_tag="RHX88_004284"
     CDS             2330..2551
                     /locus_tag="RHX88_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232888.1"
                     /translation="MNYAGHENLRAEVAEVANAMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            2564..2998
                     /locus_tag="RHX88_004285"
     CDS             2564..2998
                     /locus_tag="RHX88_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDV2232889.1"
                     /translation="MYGTCKTLCRELAAKYPGDTPLMLLIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSAAAMEIISNVSENRQVTVSAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNTHEND"
     gene            3049..3828
                     /locus_tag="RHX88_004286"
     CDS             3049..3828
                     /locus_tag="RHX88_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232890.1"
                     /translation="MNENCTLNALICRHARNLLLAQGWPEETDVDQRDPQNYPGWISI
                     YVRLDAPRLATLLVNRHDCVLPPVLASAMQKLTGTGAELVLSGSQWQSLPVLPADGTQ
                     VSFPYAGEWLIEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRR
                     FRLVVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVCECVEHILASGLVCD
                     VLRIPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
BASE COUNT          867 a         1191 c         1291 g          954 t
ORIGIN      
        1 cacacagtca aatgtcctta ttgtgactaa aaaacgctca gctctctgag tgtgtgcctg
       61 tatgcctgct gtggactgct gtgtccagct tatccacagc attatgcgca cggttctgtg
      121 gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa ccgggctgca tccgatgcaa
      181 gtgtgtcgct gttgccggcc tcctcacccg gtcacgtttc gtcgttcctc ctcctcgcgc
      241 tgcggcttcg gggccgcacc tgcattcgta tgcggtcgcc cggttacagg tgcggcacgg
      301 cctgatggag gccgcatgta agaggagaat tcccatgcca aactggtgcg ccaatcgtat
      361 gtatttttct gccgaaccgg cacagattgc tgagattaaa cgactggcca gcggtgccgt
      421 cacaccgttt tatcgccgcg ccacaaatga aggtattcag ttgtttctgg ccggaagtgc
      481 cggacttctg caaaccactg aagatgtgcg gtttgaacca tgccccggac tgagcgctgc
      541 cggacgtggc gttgtatcga cggaaaatat tgcgttcacc cgctggctga cactcctgca
      601 ggacagtgta ctgctggatg agcaaaactg cctgatgctg catgaactct ggctgcagag
      661 cggtactgga cagcgtcgct gggaaggatt accggatgat gccagggaaa ccatcactgt
      721 gcatttcacc gcaaaaagag gtgactggtg tgacatctgg ggtaacgagg atatatcggt
      781 gtggtggaac cgtctgtgtg acaacgtact gccggaaaaa accatgccgt ttgacctgtt
      841 gacggttctg ccgacccgcc tggatgttga agtaaatggc tttaacggtg gtgttctgaa
      901 cggtgttcct tctgcatatc actggtatac agaacggtat ggcgtgaagt ggccttgtgg
      961 gtatgacctg aatatcagca gtcagggaga aaacttcatt caggttgatt tcgacacgcc
     1021 gtggtgtcag ccggaaagcg acgttattgc agaattaagc cgccgtttca gctgcacgct
     1081 ggaccactgg tttgccgaac agggctgtga tttctgtggc tggcagttgt atgagcgcgg
     1141 agagctcgtt gatgtgctgt ggggggaact ggaatggtct tccccgacag atgacgatga
     1201 gctgccggaa gtcaccggac ctgcgtggat agtcgacaat gtggcgcatt atggcggatg
     1261 aagtatgacg gagacgggcg ggcaaccgcc cgtttctttt ccggtaaagg atgtcgccgt
     1321 gctcctcttt ttactgcgcc ggctgacgcg gcgcggcata aacgctgcct gtggtcagtg
     1381 tcccgcgtcc ggtgggtacg ggacgggcgc ttttaccgct cccggctggc gtcggtgaca
     1441 gtgtacgcca gcctgtcgcc cttttctgat gagcgccctt caagccgctt tcgcggcata
     1501 gccttgccgt caaaaagacg acggctgcgg tattccacgg tcggcctgac ccgttaccgg
     1561 acgcggtgaa cagcccacag gcagcgggga acgggcacca caggggtgcc ctcccggtgc
     1621 cttctgtaag agaaggagtg ttgttatgtc ccgttttgtc ctcggtaact gcatcgatgt
     1681 tatggcccgt attcctgata acgccattga tttcatactg actgacccgc catatctcgt
     1741 cggtttccgt gaccgttccg ggcgcaccat cgccggtgat aaaaccgacg aatggctgca
     1801 accggcctgt aatgaaatgt accgcgtgct gaaaaaagat gcgctgatgg tgagcttcta
     1861 tggctggaac cgcgtcgatc gctttatggc cgcctggaaa aatgcgggat tcagcgtggt
     1921 tggccacctg gtatttacca aaacctacac atcgaaggcc gcatatgtgg gctatcgcca
     1981 cgaatgcgct tacatcctgg caaaagggcg tccggcgctg cctcagaagc cattgcccga
     2041 cgtgctgggc tggaaatatt caggcaaccg ccatcacccg acggaaaagc ctgttaccag
     2101 cctgcaacca ctgattgaga gcttcacaca cccgaacgcg attgtgctgg acccgtttgc
     2161 aggcagcggc tcaacctgtg tggctgccct tcagtccaga cgccggtata tcggtatcga
     2221 gctgcttgaa cagtatcacc gtgccggaca gcaacgactg gccgccgtgc aacgggccat
     2281 gcagcagggg gccgcgaata ataactggtt tgaaccggag gctgcgtaaa tgaactatgc
     2341 cggacacgaa aaccttcgtg ctgaagtggc ggaggtggcc aatgccatgt gtgaccttcg
     2401 ggccaggctg aacgatatgg agcatcgctg tcgctttgat tccgatgtgc tcgttgaacg
     2461 cctggcacgc cagacgctgt atcgcgccaa tcgcctgttc atggaggcat ataccgaaat
     2521 tcttgaactg gatgcgtgct tcaaagatta agggggagac gaaatgtacg gaacatgcaa
     2581 aacgctctgc cgggagctgg cagcaaagta tccgggagac acgccgctga tgctgcttat
     2641 ctggtccccg gaagagattc aggccctcgc tgacggaatg gatatttccc tgtccgatca
     2701 tgaaatcagg actgtcctgg cgcgcctgga ggacatcccg gaagaccagc ggactgaatc
     2761 cggtatttct tccgccgctg cgatggagat tatcagcaac gtgagcgaaa accgccaggt
     2821 gaccgtctct gctgaactgc tggcgtccct gattcagact gctgaacagg cactgtggaa
     2881 acgtgaatgg gccgcccggg atcatggcct cgccgtcccg gaatgcgtca cccgccgtca
     2941 ggcggtgatt aatcaggccc gcaccctgct gaaaaacaac acacacgaaa acgactgatg
     3001 ttatcgccgc ctgccctgcg ggcggcgata cagggagaac gcttaatcat gaacgaaaac
     3061 tgcacgctga acgcactgat ttgtcgtcac gcccgcaacc tgctgctggc gcagggctgg
     3121 ccggaggaga cggatgttga tcagcgagat ccacagaact atccgggctg gatcagcatt
     3181 tacgtgcggc tggatgcgcc ccggctggcg acgttactcg tcaaccgcca cgattgcgta
     3241 ctgccgccgg tcctggcttc cgccatgcaa aaactgaccg gaaccggggc ggagctggta
     3301 ctgtccggca gtcagtggca gtcgctgccg gtacttccgg cagacggaac gcaggtgtct
     3361 ttcccgtatg ccggcgaatg gctgatcgaa gacgaaatca gggctgtact tgcggcggtg
     3421 cgcgatgcgg tacgcagtgt cagttgccgg gtggcagaag atgcacggcg tatccgggcg
     3481 gcgctgacca ccaccggtca gacgttgctg acccgccaga cgcgccgctt tcgtctggtt
     3541 gtgaaggaaa gcgattaccc ctgctggctt gatgaagatg acgaaaatct gcccgtggtg
     3601 ctcgatgcca ttctgaaccg tggcgcacgt ttttcctctg tggaaatgta cctggtctgt
     3661 gagtgtgtgg agcatatcct ggccagtggt ctggtctgtg atgtgctgcg tataccagat
     3721 gaaccgtccc gtcgctggtt tgaccgggat attctgcggg aggttgttct ggaggcacgg
     3781 gatgaaatcc gcagcatggc ggatgcactg gcgaaaatcc ggaaatgatg acgctggcgg
     3841 ggaaaaatct ccgccttttc cagccggtcc tcggacagag cacaaaggaa gtcgtcgccg
     3901 ggtccgcttt acccggccat gcagggcatg gccttgtggt ttttcagtta tgtggcctca
     3961 gcgtcgtgtg cgggctgtgc cgtgcctcca tcttagccgg gctggcgttg atgcaagggt
     4021 acgcttcgcc gctgcggtca cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt
     4081 tcacccgctc agatttcgcg gtctcaccct gcaactcccg tcagccgtgt gcgtcaggct
     4141 gcggcttccc ttgcgcccct gcatccccgc ctgttcgccc ggcttttatg gaggcacggc
     4201 accgcccggt gtcgcggaat atgtgaacaa cagaggaaaa catcatgcaa tatgcgaaac
     4261 ctgtcactct gaatgttgaa gagtgcgacc gtctctcctt tct
//
LOCUS       DAPGWD010000242         4226 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPGWD010000242 DAPGWD010000000
VERSION     DAPGWD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.244"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            172..690
                     /locus_tag="RHX88_004287"
     CDS             172..690
                     /locus_tag="RHX88_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDV2232891.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            900..3041
                     /locus_tag="RHX88_004288"
     CDS             900..3041
                     /locus_tag="RHX88_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV2232892.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3117..>4226
                     /locus_tag="RHX88_004289"
     CDS             3117..>4226
                     /locus_tag="RHX88_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV2232893.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPTKK
                     DSPNYGSPVNPLLGAKVLPVETDLALPGPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESFWLARGGVLKQHKGHPLA
                     RLWRALPEAVRLSPHTYMMAVSTTGQWLILGWPERVPEADEVPPPEPPAYRVLTGVVD
                     GFGRSLIFHREAAGELAGEITGVTDGAGRRFHLALSTQAQRAEAFRKQRVTSLSSPAG
                     PRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGE
                     LRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT          989 a         1122 c         1267 g          848 t
ORIGIN      
        1 tttaatgatt ttaaaaatag tttttacgac aaccgtaaat tctagctcgg tttgtatgta
       61 aaaaatactg aaaaatgatg cggttccgtg aagtgagctg catctgcctg tctgggatta
      121 acggcaggtc attgtaagta gtaattaacc acgttaacaa ggagtttaca aatgccaaca
      181 ccgtgttata tctccatcac tggtcagacc cagggaaata ttactgcagg tgcatttacc
      241 gctgattctg tcggcaacat ctatgtgcag gggcacgaag acgagatgct ggtgcaggaa
      301 ttcctgcata acgttactgt cccgaccgat cctcagtccg gccagcctgc gggtcagcgt
      361 gctcacaagc cgttcatttt taccgtggcg ctgaacaaag ctgttccgct gatgtacaac
      421 gcactggcct ccggcgaaat gctgccaacc actgaacttc actggtggcg tacctctgtg
      481 gaaggcaaac aggagcatta tttcaccacc cgcctgaccg attcgacgat tgtcgatatg
      541 aaactgcata tgccgcattg ccaggatccg gcgaaacgcg agttcaccca gttgctcgaa
      601 gtttcactgg cctaccgcaa aatagagtgg gaacacgtca agtctggcac ctccggtgcc
      661 gacgactggc gcgcaccgct ggaagcataa gttaagccaa cagcatccgg ctatcaggcc
      721 ggatgctgtt tttgtattaa gccgtaaaaa gaaaggttgt cagtaagggg ttacgttcac
      781 gggagatggc ggatgccgga aacttcggcg gtagcgtgcc tgaacgcaag cgtaacactg
      841 tactgtcaca gccgctttaa cagcgaactg tctgtttttc cttaaccccg gaggttgcta
      901 tgtcaaccgg attacgtttc acgctggaag tggacggcct gccaccggat gcttttgcgg
      961 tggtctcctt tcatctgaac cagtcactct cttcgctctt ttccctcgat ctctctctgg
     1021 tcagccagca gtttctctcc cttgaatttc agcaaatact ggacaaaatg gcctacctga
     1081 cgatatggca gggcgatgac gtacagcgcc gggtgaaagg tgtggtgacc tggtttgaac
     1141 tgggggagaa cgacaaaaac cagatgctgt acagcatgaa ggtgtgccca ccgctgtggc
     1201 gcacagggct gcgccagaac ttccgtatct tccagaatga ggacatcgaa agcatcctcg
     1261 cgacgatcct gaaagaaaac ggtgtgaccg agtggagtcc gctgttcagc gagccgcatc
     1321 cttcccgtga gttttgtgtc cagtacggcg aaaccgatta cgatttcctg tgccggatgg
     1381 cggcggagga aggcatcttc ttttatgagg agcacgcgca aaaaagtacc gaccagagcc
     1441 tggtcctgtg cgacaccgtg cgttatctgc cggagtcctt tgagatcccc tggaatccga
     1501 acacccgtac cgaggtgagc accctctgca tcagccagtt ccgctacagc gcacaaatcc
     1561 gtccttcttc cgtggtgaca aaagactaca cctttaaacg tcccggctgg gcagggcgtt
     1621 ttgaccagga gggccagcac caggactacc agcgcacgca gtatgaagtg tatgactacc
     1681 ccggacgttt caagggcgcc cacgggcaga actttgcccg ctggcagatg gacggctggc
     1741 gaaacaacgc agaagtggcg cgcggaacaa gccgttcgcc ggagatatgg ccgggacggc
     1801 gaattgtgct gacggggcat ccgcaggcga acctgaaccg ggaatggcag gtggtggcga
     1861 gcgatcttca cggcgaacag ccgcaggcgg taccgggacg caggggttca ggcaccacgc
     1921 tggataacca ttttgcggta ataccggcag acagaacatg gcgaccacag ccgttgctga
     1981 aaccgctggt ggacggcccg cagagcgccg tcgtgacggg accggcaggc gaggaaatct
     2041 tctgtgatga acatggccgc gtgcgggtga aatttaactg ggaccgttat aacccgtcaa
     2101 accaggacag ttcatgctgg atccgtgtgg cacaggcgtg ggcaggcacc ggattcggta
     2161 acctggcgat accgcgtgtg ggtcaggagg tgattgtgga cttcctcaac ggcgatccgg
     2221 accagccgat cattatgggg cgcacctacc accaggaaaa ccgcacaccc ggcagcctgc
     2281 cgggaacaaa gacgcagatg accattcgtt cgaaaaccta taagggcagc gggtttaatg
     2341 aactgaagtt tgacgatgcg acagggaaag aacaggtcta catccacgcg cagaagaaca
     2401 tgaacaccga ggtgctgaat aaccgcacca ctgatgtgat aaacaaccat gccgaaaaaa
     2461 taggtaacaa ccaggcgatc accgttacca ataaccagat ccagaacatt ggcgttaatc
     2521 agatacagac ggttggtgtc aaccaggtgg aaacggtggg cagtaaccag attatcaaag
     2581 tgggatcaaa ccaggttgaa aaggtgggga tcattcgtgc gctgacggtg ggtgtagctt
     2641 accagacgac ggtaggcggc attatgaata cctcggtggc gttgttgcag tcctcacagg
     2701 tagggctgca taaatcactg atggtgggaa tgggctacag cgtcaatgtg gggaataacg
     2761 tcaccttctc ggtgggcaag acgatgaagg aaaacaccgg acaaacagca gtttattctg
     2821 ccggtgagca tcttgaactc tgctgtggta aggcaaggct ggtgctgacg aaggacggaa
     2881 gcatatttct caacggtacg cacattcatc tggaagggga gtcggatgtg aacggtgatg
     2941 cgccagtgat taactggaac tgtggtgcca cacaacctgt accggatgcg cctgtgccga
     3001 aagatttacc ccccggaatg ccggatatgc ggcaattttg agtcaggtgc aaaaaattaa
     3061 aacggaactg gtgcagtgac cagatgaacc atattaaaag cagaaggaat tgatttatgg
     3121 gcggcggaaa accggcggcg cggcagggtg acatgacccg caagggactg gatattgtgc
     3181 agggttcagc aggggtgctg ataggtgcgc caaccggtgt ggcctgctcg gtgtgtccca
     3241 caaaaaagga cagtccgaac tatggcagcc cggtgaaccc gctactcggt gcgaaggttc
     3301 tgcctgttga aaccgacctg gcactgcccg gcccgctgcc gtttattctt ttccgcgcct
     3361 acagcagcta ccggaccagg acgcccgcgc cagtgggcgt gtttggtccc ggctggaaag
     3421 cgcccttcga tatccgctta cagatacgcg atgaaggcct gatactcaac gacagcggtg
     3481 gtaggagcat tcactttgag ccgttgtttc ccggcgagat aagctacagc cgcagcgagt
     3541 cgttctggct ggcaaggggc ggagtgctga aacaacacaa aggccatccg cttgcgcggc
     3601 tctggcgggc gttgccggaa gcggtccgtc tgagtccaca cacctatatg atggctgtca
     3661 gcacaaccgg gcagtggctg atactcggct ggccggagcg tgtgccggag gcggacgagg
     3721 tgccgccgcc ggagccgccc gcataccggg tgctgacggg cgtggtggac ggcttcgggc
     3781 gctcactgat tttccaccgc gaggccgcag gcgagctggc tggcgaaatc accggggtga
     3841 cggacggcgc gggccgccgt tttcacctgg cactgagcac acaggcgcag cgggcggaag
     3901 ccttccgtaa acagcgcgtc acgtctttat cctcccctgc cggtccccgt tctgtttcct
     3961 cttctcaggt tttccccgac acgctgcccg ccggtacaga gtacggtgcc gataacggta
     4021 tccggctgga ggcggtgtgg ctgacacacg acccggccta cccggatgaa cagccgactg
     4081 cgccgctggc gcgctacacg tacacggcca gcggagaact gcgggcggtg tatgaccgca
     4141 gcgggacgca ggttcgcggg tttacttatg atgcggagca cgccgggcgg atggtggcac
     4201 accattatgc ggggaggccg gagagc
//
LOCUS       DAPGWD010000243         4203 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPGWD010000243 DAPGWD010000000
VERSION     DAPGWD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.245"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            42..1046
                     /gene="yeeE"
                     /locus_tag="RHX88_004290"
     CDS             42..1046
                     /gene="yeeE"
                     /locus_tag="RHX88_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDV2232894.1"
                     /translation="MQRGRFCLTGGFRDMYIVKNNRMFYALLIAISVQSVGVFALIQA
                     GLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMS
                     AVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVLLVITLWVVMKELKKPKLK
                     VATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITSPTANI
                     LQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGVLMGF
                     GASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN
                     "
     gene            1060..1287
                     /locus_tag="RHX88_004291"
     CDS             1060..1287
                     /locus_tag="RHX88_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDV2232895.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1330..2757)
                     /gene="sbcB"
                     /locus_tag="RHX88_004292"
     CDS             complement(1330..2757)
                     /gene="sbcB"
                     /locus_tag="RHX88_004292"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDV2232896.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEATFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTHQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            2966..4132
                     /gene="dacD"
                     /locus_tag="RHX88_004293"
     CDS             2966..4132
                     /gene="dacD"
                     /locus_tag="RHX88_004293"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDV2232897.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEAKKLLR
                     WGQQNFTTVQILHRRKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLN
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
BASE COUNT          990 a          966 c         1120 g         1127 t
ORIGIN      
        1 tattaagcgg gctaatttgt ggtgctctgc tgggatttgt catgcagcgt gggcgttttt
       61 gcctgacagg tggttttcgc gatatgtata tcgtgaaaaa taatcgcatg ttttacgcct
      121 tgctgattgc aatttcggta caaagcgtgg gggtttttgc gttaattcag gcgggcctac
      181 tgacttacga agccggggcg ttcccgtggc ttggtactgt tataggtggg tatatcttcg
      241 ggctgggaat tgttctggcg ggtggatgtg ccaccgggac ctggtatcgc gcgggtgagg
      301 gattgatcgg cagttggatc gcgcttttca cttatatggt gatgagtgcg gtgatgcgtt
      361 ctccacatgc cagtggttta aatcaaacct tgcagcacta cagtactgaa cataactcta
      421 ttgctgagac ttttaatttg tctgtgtggc cgttggttgc cgttttgctg gtgataacgc
      481 tctgggtggt gatgaaagag ttgaagaagc caaaactcaa agtcgcgacc ttaccgccgc
      541 gccgaaccgg gatcgctcat attctgtttg aaaaacgctg gcatcccttt gtcactgctg
      601 tactcatcgg tttgattgcg cttttagcct ggcccctgag tgaagcaacc ggacgcatgt
      661 ttgggttagg aatcacttcc ccaacggcca atattctgca atttctggtc gcgggtgacg
      721 tgaaatacat taactggggc gttttcctgg tgttagggat cttcgtgggg tcatttattg
      781 cagccaaagc gagccgtgag ttccgcgttc gcgcagctga tgcacaaaca acattacgta
      841 gcgggttagg tggtgtactg atgggcttcg gtgccagtat tgcaggtggt tgctctatcg
      901 gtaatggact ggttatgact gcaatgatga cctggcaggg ctggattggc cttgtattta
      961 tgattctcgg agtctggact gcgtcctggc ttgtgtatgt tcgaccgcag cgtaaggcgc
     1021 gactggcaac tgctgcggca aattaagtaa ggtgtaaaga tggcgattaa aaagctggat
     1081 gtggtgacgc aggtctgtcc gtttccactc atagaagcaa aagcagcact ggcagaaatg
     1141 gccagcggcg atgagttggt aattgagttt gattgcaccc aggcaacaga agcaatccca
     1201 caatgggcgg cagaagaagg acatgccatc accgattatc agcagattgg cgatgctgcg
     1261 tggagcatca ccgttcaaaa agcctgaccc aacattgtcc tccgccgtat cagcggcgga
     1321 ggcttcaaac tagacgatct cttccgcgta ctgccaaagc gcttttaaca gcgccacttt
     1381 ttctttatcg tcggcatact gctgcgccag catctgaatc tcttctgcat agccttgtaa
     1441 aaactcgggt gtgaatacct ggcggcggtg ctccagccag cgttgctgct cggcataatc
     1501 cagcgtcccc gggaagttgc gcgcccgata attgaacaac agtttttcaa ttcgtttatc
     1561 aacaaaagtg atatccagtg ccggtaaatt acgtggctcg gtttccagca caattttcat
     1621 tgctgcgcga tcggcgtcac tgaaaaagcc gttataaagc tgtgcatcca cgttatctga
     1681 aggcgtaaac ggttcggctt ccgcgaatat cgccaccact ttttcgcgca cttgcggatt
     1741 ttcacgcaga atttttaggt tatcgaggca atgctgacga ttaattccca gtcggtcggc
     1801 atcttccggg cgtagcgtat tcgcctgggc cagcaccgga catttattga tatgcaccag
     1861 cttaaccgga acggcggcgt tatcgccaag atcggttttt gcggtatata aacgctcgcg
     1921 caatgtgtcg ctatccagtt ccagtaatgg cgaaatgtct cctgccaaat ccaccataat
     1981 tacggcattg cgattttctg gatgccacgc cagcggtgcc acccagctgg tattgccgcg
     2041 ccatgcccca aacattccgg aaacgtgaac caggggtttc atctgcggaa catcaatcaa
     2101 cgccatcagt ttgtgtttat tacgatgggt aaagagataa tcaaacaggc gtggctgatg
     2161 cgtttttacc agtttcgcca tcgcaatagt ggcgtacaca tcagccatcg catcgtgggc
     2221 gttgctatgt tcaataccat tcgctttggt taaatgctca aggcgaaagc tcggtagacc
     2281 gtcatcattt tcaggccagt ttattccttc cgggcgcagg gcataacagg cacgcataac
     2341 atccagtaaa tcccagcgcg agttatcatg ctgccagctc caggcgtaag gatcgtagaa
     2401 attacgataa aaaacgttgc gtgtgacttc gtcgtcgaaa cgcacattgt tgtagcccag
     2461 aatacaggtc ttcggtacgg taaaaagcga gtgaatacgg gcggcaaacg tggcttcgtt
     2521 ttctcctttc gcccgtgctt cctgcggggt aataccggta attaatactg ctccaggctg
     2581 gggtaaatag tcatccgcgg gcttgcagta aaagacttcg ggttcgccga tgacattgaa
     2641 ttcgttatca gtgcgaatgg ctgcgaactg tgcagggcga tctaacgcgg ggtgcgttcc
     2701 aaaggtttcg taatcgtgaa acaaaaaggt agattgttgc ttaccgtcat tcatcattag
     2761 gttaaatccg ttatttctgc tgtctgccag agtatcaaat atcaccgtgc taatcagctt
     2821 tagcgcgaca atttggcagc gagtgacaac agatcatgtc agataaaaat gagatagtag
     2881 tcacattttc ttgcacttta ttccagccag ttcataagta tttccgtaaa aagaacagct
     2941 atttgaaaca cctgagggtt tgctgttgaa acgccgtctt attattgctg cttctttgtt
     3001 cgtttttaat ttatcgtctg gttttgcggc ggaaaacatt cctttttcac ctcagcctcc
     3061 agagattcat gccgggtcct gggtattgat ggattacacc accggtcaga ttctcaccgc
     3121 gggtaatgag catcaacagc gcaatcccgc cagcctgaca aagctgatga cgggctatgt
     3181 cgtggatcgc gctatcgata gtcatcgcat tacgccagac gatattgtca ccgtggggcg
     3241 tgatgcgtgg gcgaaagata atccggtgtt tgtcggttct tcactgatgt ttttgaaaga
     3301 gggcgatcgc gtatcggtac gtgatttaag ccgaggttta attgtggatt ccggaaatga
     3361 cgcgtgtgtt gctctggctg actatattgc cggtgggcaa cggcagtttg ttgaaatgat
     3421 gaacaactat gccgagaagc tgcatctcaa ggatacgcat tttgaaacag tgcatggtct
     3481 ggatgcacct ggccagcata gctcggctta tgatttagcc gtgctttctc gcgctatcat
     3541 ccacggcgag cccgagtttt atcatatgta cagtgagaaa agcctcacct ggaacggtat
     3601 cacccagcaa aaccgtaacg ggttattgtg ggataagacc atgaatgttg acggcctgaa
     3661 aacgggccat acttctggtg ccgggtttaa tcttattgct tcggctgtag acgggcagcg
     3721 tcgcctcatt gcagtggtaa tgggtgctga cagcgcaaaa ggtcgtgagg aagaggcaaa
     3781 aaaattactg cgttgggggc aacaaaactt tactacggtg caaattttgc accgtaggaa
     3841 aaaggtcggt acggaacgca tctggtatgg tgataaagaa aatatcgccc tgggaacgga
     3901 acaagagttc tggatggtgc taccgaaagc cgaaattcca catatcaaag ccaaatatac
     3961 ccttaatggt aaagagctca ccgcgccaat tagcgcccat cagcgggtag gggaaattga
     4021 actttacgac cgtgataaac aggtggcgca ctggccgctg gttaccctgg aatctgtcgg
     4081 ggaaggcagc atgttttctc gcctgagtga ttatttccac cataaggcct gacctttctt
     4141 ttgcagcaga ctggcaggag tgcgagtctg ctcgcataat caacactcat tccttgtggt
     4201 ttt
//
LOCUS       DAPGWD010000244         4142 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPGWD010000244 DAPGWD010000000
VERSION     DAPGWD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.246"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            135..1238
                     /gene="ompC"
                     /locus_tag="RHX88_004294"
     CDS             135..1238
                     /gene="ompC"
                     /locus_tag="RHX88_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV2232898.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1350..2405
                     /gene="apbE"
                     /locus_tag="RHX88_004295"
     CDS             1350..2405
                     /gene="apbE"
                     /locus_tag="RHX88_004295"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDV2232899.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2479..3543
                     /gene="ada"
                     /locus_tag="RHX88_004296"
     CDS             2479..3543
                     /gene="ada"
                     /locus_tag="RHX88_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDV2232900.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESKRGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3543..>4142
                     /gene="alkB"
                     /locus_tag="RHX88_004297"
     CDS             3543..>4142
                     /gene="alkB"
                     /locus_tag="RHX88_004297"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDV2232901.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLT"
BASE COUNT         1008 a         1073 c         1112 g          949 t
ORIGIN      
        1 gttttagtat catattcgtg ttggattatt ctgcattttt ggggagaatg gacttgccga
       61 ctgattaatg agggttaatc agtatgcagt ggcataaaaa agcaaataaa ggcatataac
      121 agagggttaa taacatgaaa gttaaagtac tgtccctcct ggtcccagct ctgctggtag
      181 caggcgcagc aaacgctgct gaagtttaca acaaagacgg caacaaatta gatctgtacg
      241 gtaaagtaga cggcctgcac tatttctctg acaacaaaga tgtagatggc gaccagacct
      301 acatgcgtct tggcttcaaa ggtgaaactc aggttactga ccagctgacc ggttacggcc
      361 agtgggaata tcagattcag ggcaacagcg ctgaaaacga aaacaactcc tggacccgtg
      421 tggcattcgc aggtctgaaa ttccaggatg taggttcttt cgactacggt cgtaactacg
      481 gcgttgttta cgacgtaact tcctggaccg acgtactgcc agaattcggt ggtgacacct
      541 acggttctga caacttcatg cagcagcgtg gtaacggctt cgcgacctac cgtaacactg
      601 acttcttcgg tctggttgac ggcctgaact ttgctgttca gtaccagggt aaaaacggta
      661 gcgtaagcgg cgaaggcatg accaacaacg gtcgtggtgc tctgcgtcag aacggcgacg
      721 gcgttggcgg atctatcact tatgattacg aaggcttcgg tatcggtggt gcgatctcca
      781 gctccaaacg tactgatgat cagaacagcc cgctgtacat cggtaacggc gaccgtgctg
      841 aaacctacac cggtggtctg aaatacgacg ctaacaacat ctacctggct gctcagtaca
      901 cccagaccta caacgcaact cgcgtaggtt ccctgggttg ggcgaacaaa gcacagaact
      961 tcgaagctgt tgctcagtac cagttcgact tcggtctgcg tccgtctgta gcatacctgc
     1021 agtctaaagg taaaaacctg ggtgtcatca atggtcgtaa ctacgacgac gaagatatcc
     1081 tgaaatatgt tgatgttggc gcgacctact acttcaacaa aaacatgtcc acctacgttg
     1141 actacaaaat caacctgctg gacgacaacc agttcactcg tgacgctggc atcaacactg
     1201 ataacatcgt agctctgggt ctggtttacc agttctaatc tcgattgata tcgaacaaag
     1261 ggcctgcggg cccttttttc attgttttca gcgtacaaac tcattttttt ggtgtactct
     1321 tgcgaccgtt cgcatgagga taatcacgta tggaaataag ctttacccgc gtggcactgc
     1381 tggctgccgc gctcttcttt gttggttgcg atcaaaaacc acaacccgcc aaaacccacg
     1441 ctactgaagt taccgttctt gaaggcaaaa ccatgggtac cttctggcgt gccagcatcc
     1501 cgggcattga tgccaaacgc agtgccgaac ttaaagaaaa gattcagacc cagctggacg
     1561 ccgacgatca gctgctttcg acctataaaa aagattccgc gctgatgcgc tttaacgact
     1621 cgcaaagttt gtcgccgtgg ccagtaagtg aagcgatggc cgaaatcgtc actacctcgc
     1681 tgcgcattgg cgcgaagacc gatggtgcga tggatattac cgtcgggccg ctggtgaatc
     1741 tgtggggctt tggcccggaa caacagccgg ttcaaattcc gagccaggaa cagatcgatg
     1801 cgatgaaagc caaaaccggc ttacagcacc tgacggtcat caatcagtcg catcagcaat
     1861 atctgcaaaa agacctgccg gatttatata tcgatctctc caccgtcggt gaaggttatg
     1921 cggcggatca cctggcacgc ttgatggagc aggaagggat ttcccgctat ctggtgtcgg
     1981 tgggcggcgc gctgaacagc cgtggtatga acggtgaagg ccagccgtgg cgggtagcga
     2041 ttcaaaaacc aaccgataaa gaaaacgcgg ttcaggctgt ggtggatatc aacggtcatg
     2101 ggattagcac ctctggcagc taccgtaact attacgaact ggacggcaaa cgtctttctc
     2161 atgttatcga tccgcaaacc gggcgtccca tcgaacacaa tctggtatcc gtgacggtga
     2221 ttgctccgac ggcgctggaa gccgatgcct gggatactgg cttgatggta ctcgggccgg
     2281 agaaagccaa agaggttgtt cgccgggaag ggctggcggt ttatatgatc accaaagaag
     2341 gcgatagctt taaaacctgg atgtcaccac agtttaaaag cttccttgtc agcgaaaaaa
     2401 attaaagcgc aagattgctg gtttttgcgt gatggtgacc gggcagacta aaggctatcc
     2461 ttaaccaggg agctgattat gaaaaacgcc acatgcttaa ctgacgatca acgctggcaa
     2521 tctgtcttag cccgcgaccc gaatgccgac agcgaattcg ttttcgccgt gcgcaccaca
     2581 ggcatctttt gccgtccgtc ttgccgcgcc agacatgcct tgcgggaaaa tgtctccttc
     2641 tacgcaaatg cctgcgaggc actcgccgcc ggatttcgcc cctgcaaacg ctgccaacct
     2701 gataaagcca atgcccagca acatcggttg gataaaatca cccacgcgtg tcgactgctg
     2761 gaacaggaaa cgcctgtaac gctggaagcc ttagccgacc aggtggcgat gagtccattt
     2821 catctacatc ggttgtttaa agcgactacc ggaatgacgc ctaaagcctg gcaacaggcc
     2881 tggcgcgctc gccgtttgcg cgaatcgctg gcgaaagggg agagcgtgac gacgtctatt
     2941 cttaacgccg gattccccga cagcagcagt tactatcgca aagctgacga aacgctgggc
     3001 atgacggcta aacaattccg tcacggtggc gaaaatctgg cggtgcgtta cgcgctggct
     3061 gattgtgagc tgggtcgttg cctggtggca gaaagcaagc gggggatttg cgcgatattg
     3121 ctgggcgatg atgacgcgac actaatcagc gagttgcagc agatgtttcc cgctgccgac
     3181 aacgcgcctg ccgatctgat gtttcagcaa catgtgcgtg aagtgatcgc cagcctcaat
     3241 caacgcgata cgccgctgac gttaccgctg gacattcgcg gcactgcttt tcagcaacaa
     3301 gtctggcagg cactgcgcac gataccttgc ggtgaaaccg tcagttatca gcaactggct
     3361 aacgccatcg gcaaaccgaa agcggtacgg gccgttgcca gcgcctgtgc cgccaacaag
     3421 ctggctatca taataccctg tcatcgggtg gtccgtggtg atggcacact ttccggttac
     3481 cgctggggcg tgtcgcgtaa agcgcaactg ctgcgccgcg aagctgaaaa tgaggagagg
     3541 taatgttgga tctgtttgcc gatgctgaac cgtggcaaga gccactggcg gctggagcgg
     3601 taattttgca ccgttttgct tttaacgctg cggagcaact gatccgcgat attaatgacg
     3661 ttgccagcca gtcgccgttt cgccagatgg tcacccccgg gggatatacc atgtcggtgg
     3721 cgatgaccaa ctgtgggcat ctgggctgga cgacccatcg gcaaggttat ctctattcgc
     3781 ccattgatcc gcaaacaaat aaaccgtggc ccgccatgcc acaaagcttt catgatttat
     3841 gtcaacgtgc ggctacggcg gcgggctatc cggatttcca gccagatgct tgtcttatca
     3901 accgctacgc tcctggcgcg aaactgtcgc tgcatcagga taaagacgaa ccggatctgc
     3961 gcgcgccgat tgtttctgtt tctcttggct tacccgcgat ttttcaattt ggtggcctga
     4021 aacgtaatga tccgctcaaa cgtttgttgt tggaacatgg cgatgtggtg gtatggggcg
     4081 gtgaatcgcg gctgttttat cacggcattc aaccgctgaa agcggggttt catccactca
     4141 cc
//
LOCUS       DAPGWD010000245         4136 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPGWD010000245 DAPGWD010000000
VERSION     DAPGWD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.247"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2023
                     /locus_tag="RHX88_004298"
     CDS             <1..2023
                     /locus_tag="RHX88_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2232902.1"
                     /translation="YAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTS
                     AERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARG
                     TAQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSV
                     PVLQRAGYTRYALAMGEYRSGNNLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAF
                     NLGIGKDLGLFGAFSFDITQANTTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRY
                     STQGFYNLSDSAYSRMSGYTVKPPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGN
                     YGTTFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFT
                     LNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGN
                     THGGNTSSGTSGYSSLNYRGAYGNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQP
                     LGDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRVALNANSLADNVEL
                     DETVVTVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAEN
                     GQVYLTGLPQSGKLQVSWGKDKNSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            2037..2567
                     /locus_tag="RHX88_004299"
     CDS             2037..2567
                     /locus_tag="RHX88_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2232903.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPVVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            2580..3083
                     /locus_tag="RHX88_004300"
     CDS             2580..3083
                     /locus_tag="RHX88_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2232904.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            3142..4056
                     /locus_tag="RHX88_004301"
     CDS             3142..4056
                     /locus_tag="RHX88_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2232905.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
BASE COUNT         1182 a          884 c          952 g         1118 t
ORIGIN      
        1 ctatgcatat ttgaatttac agagtggcat caacattggt agttggcgac tacgcgataa
       61 ctcaacgtgg agttataaca gtgggagtag caattcatct gacagcaata aatggcagca
      121 tatcaatacg tcggctgaac gtgacattat tcccttacgc tcacggttaa cggtaggtga
      181 tagttatacc gatggcgata tttttgatag tgtgaacttt cgtggcctca aaataaattc
      241 aacagaagcg atgttgcccg atagccaaca tggtttcgct ccggtgattc atggtattgc
      301 ccgtggcacc gcacaagtga gtgtaaaaca gaacggatac gatgtttatc agactactgt
      361 tccacccggc ccttttacta ttgatgatat caactctgcc gccaatggtg gtgatctgca
      421 agtaaccata aaagaggcag acggcagtat tcagacatta tatgttcctt attcgtctgt
      481 tccggttctc caacgtgctg gatatacgcg ttatgcgctt gccatggggg aatatcgtag
      541 tggaaataac ctgcaaagct cccccaagtt catacaaggt agcttgatgc atggactgga
      601 aggaaactgg acaccttatg gcggaatgca aattgcagaa gattatcagg ccttcaacct
      661 tggtattggt aaagatttag gactttttgg tgccttttct ttcgatatca cgcaggccaa
      721 tacgacactt gcagatggca cccgtcacag cgggcaatcg gttaaatccg tctacagcaa
      781 atccttctac cagacaggaa ccaatatcca ggtcgcagga tatcgctatt ctacgcaagg
      841 tttttataac ttatccgaca gtgcctacag tcgaatgagt ggttacaccg ttaagcctcc
      901 taccggagac agcaatgagc agacacaatt tattgattat tttaatctgt tctacagtaa
      961 gcggggtcag gaacaaataa gcatctctca gcagcttgga aattacggta cgacattttt
     1021 cagtgccagt cgccaaagtt actggaacac gtcacgcagc gaccagcaaa tatcatttgg
     1081 attaaatgtg ccgtttggtg atataacgac ttcgctgaat tacagctatt ccaataatat
     1141 atggcaaaac gatcgggatc atttactcgc ttttacgctt aatgttccct tcagtcattg
     1201 gatgcgtaca gacagtcagt cggcatttcg taattcaaac gccagttaca gtatgtcaaa
     1261 cgatttgaaa ggcggcatga ccaatctatc gggggtttat ggcactctgc tgccggataa
     1321 taacctgaat tatagcgttc aggtcggtaa cacccacgga ggtaatacat cgtctggcac
     1381 cagtggttac agttctctta attatcgtgg agcttacggt aatacgaatg tcggttacag
     1441 tcggaatggt gacagcagcc agatttatta cggaatgagt ggtgggatta ttgctcatgc
     1501 tgatggcatc acctttggac agccgctggg cgacacaatg gttctggtta aggctcctgg
     1561 cgctgataat gtcaaaatag agaaccagac cggaattcat accgactggc gtggctatgc
     1621 catattacca tttgcgacag aatatagaga aaaccgtgtt gctcttaacg cgaattccct
     1681 tgcagataat gttgaactgg atgaaaccgt ggtcactgtc atcccaactc acggtgctat
     1741 tgccagagca acatttaatg cacaaatcgg cgggaaagta ttaatgacgt tgaagtacgg
     1801 taataagagc gttccattcg gtgcaattgt cacacacgga gagaataaaa atggcagcat
     1861 tgtcgcagaa aacggtcagg tttatctgac tggacttcca cagtcaggga aattacaggt
     1921 ttcatggggc aaagataaaa actcaaactg tattgtcgag tacaagcttc ctgaagtttc
     1981 tcctggtacc ttactgaacc agcagacagc aatctgtcgc taaggaacaa tagattatga
     2041 aatacaataa cattattttc ctcggtttat gtctggggtt aaccacctat tctgctttat
     2101 ccgcagatag cgttattaaa attagcgggc gcgtcctcga ttatggctgc acagtctcat
     2161 cggattcgct taattttacc gtagatctcc aaaaaaacag tgccagacaa tttccaacga
     2221 ccggtagcac aagtccagtc gtcccttttc agattacgtt aagtgaatgc agcaaaggga
     2281 caacgggggt tcgggttgca tttaacggta ttgaggatgc agaaaataat actttgttga
     2341 aactggatga aggaagcaat acggcctccg gtttgggtat agaaatattg gacgcaaata
     2401 tgcgtccggt gaaactgaat gaccttcatg ccgggatgca gtggatccca ctggtaccag
     2461 aacagaacaa tattttgcct tactccgctc gtctgaagtc aactcagaag tccgtcaatc
     2521 cgggactggt gagggcttcg gcaaccttta cccttgaatt tcaataatca gagtcagata
     2581 tgaaaagact tcacaagagg ttcctgttag ctacgttttg cgcgttattc acagcaactc
     2641 tccaggccgc cgatgtcact atcactgtta atggtcgggt agtcgctaaa ccctgcacta
     2701 ttcaaaccaa agaagctaac gttaatctcg gggatcttta tacgcgcaat ctgcaacaac
     2761 ctggttctgc atctggctgg cacaatatta ctttgtcatt aaccgattgt ccggttgaaa
     2821 caagtgcagt gacggcaatc gtgacaggtt caactgacaa tacgggttat tacaaaaatg
     2881 aaggtactgc cgaaaatatt cagatagagc tgagggatga ccaggatgct gcgttaaaaa
     2941 atggcgatag caaaacggtt attgttgatg agatcactcg taatgcacag tttccactta
     3001 aggcaagagc tatcacggtg aatggaaacg caagccaggg aacgatcgag gcgctaatca
     3061 atgtgatcta cacctggcaa taacgtcgat tcgattttct tagccttctt tttaaatcaa
     3121 tttttaacct taggatatat catgggtaaa acaatcagta taaaggtcct gttcggtatt
     3181 tatcttctat taatggcagg caaagttttt gccttcagtt gtaatgtcga tggcgggtca
     3241 agtattggag caggtacaac ctctgtttat gttaatctcg accctgtaat acagccgggc
     3301 cagaatctgg ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg
     3361 tacgacactg atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca
     3421 tataaaggat cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc
     3481 aacgtattgg atattggtga taagacaccg atgccattac cgttaaaact ctatattacc
     3541 ccagttgggg cggccggtgg agttgtcata aaagcggggg aagtcattgc tcgtattcat
     3601 atgtataaaa ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt
     3661 tcaaataaca atgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcact
     3721 gtggatctcc cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc
     3781 gaacaaaagc tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt
     3841 gctaacacag cacctgatgc aacaaaagca tcgggagtgg gtgtctctct gatgcgtaat
     3901 ggaaaaatac tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta
     3961 cctctgggtt taagcgcaac ctatggacaa acaggaaaca aggttgcagc cggaaccgtc
     4021 cagtcagtca ttggtgtcac ttttatctat gaataattgt gattttgatc ttagtctatg
     4081 cgctccattc acgaaaattg cgcggataat tattttgtga aggctattat cctaca
//
LOCUS       DAPGWD010000246         4088 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPGWD010000246 DAPGWD010000000
VERSION     DAPGWD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.248"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..1713
                     /gene="dpiB"
                     /locus_tag="RHX88_004302"
     CDS             55..1713
                     /gene="dpiB"
                     /locus_tag="RHX88_004302"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDV2232906.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1682..2362
                     /gene="dpiA"
                     /locus_tag="RHX88_004303"
                     /gene_synonym="citB"
     CDS             1682..2362
                     /gene="dpiA"
                     /locus_tag="RHX88_004303"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDV2232907.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2403..3788)
                     /gene="dcuC"
                     /locus_tag="RHX88_004304"
     CDS             complement(2403..3788)
                     /gene="dcuC"
                     /locus_tag="RHX88_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV2232908.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1124 a          956 c         1052 g          956 t
ORIGIN      
        1 caacatttta attacatccg tcaaagaggc tcgggacaac ccgcaaggaa aacaatgttg
       61 cagcttaacg agaataaaca gtttgcattt ttccaaagac tggcatttcc gctgcgtatc
      121 tttttgctga ttctggtgtt ctcaatattt gtcattgcag ccctggcgca atattttacg
      181 gccagttttg aggactattt aacgcttcat gtacgcgaca tggcaatgaa tcaggcgaaa
      241 attattgcct ccaatgacag tgtcatctct gcggtgaaaa cgcgtgacta caaacggctg
      301 gcgaccatcg ctaacaaatt acaaagagat accgattttg attatgtggt gattggggac
      361 cggcactcga tccgccttta ccatcctaat ccggagaaaa ttggttatcc tatgcagttc
      421 accaaacagg gcgcgctgga gaaaggggag agctacttca ttaccgggaa agggtcaatg
      481 gggatggcga tgcgcgccaa aacgccaatc tttgatgacg atggaaaagt catcggcgtg
      541 gtgtcgattg gctacctggt gagtaaaatc gatagctggc gggctgagtt tttattaccg
      601 atggcaggcg tgtttgtcgt gctgttaggg attctgatgt tgctgtcgtg gttcctggca
      661 gcgcatatcc gtcggcaaat gatgggcatg gagccaaagc aaatcgcacg cgtggtccgt
      721 cagcaagagg cgctgtttag ttcggtttat gaagggctga ttgcggtgga tccgcatggt
      781 tacattaccg ccatcaatcg taacgcaaga aagatgctgg ggctgagctc tcccggacgg
      841 caatggttgg gtaaacccat tgctgaagtg gtcaggcccg ccgatttctt taccgaacag
      901 attgatgaaa aacgtcagga tgtggtggct aactttaacg gtctgagcgt tattgccaat
      961 cgggaagcta ttcgttcagg tgatgatttg ctgggggcca ttatcagctt tcgtagtaaa
     1021 gacgaaattt ccaccctcaa tgcgcaactg acgcaaataa aacaatacgt cgagagcctt
     1081 cgtacattgc gacacgagca tctcaattgg atgtcgacgc tcaatggtct gttgcagatg
     1141 aaagagtatg atcgcgtgct ggcgatggtg cagggggagt ctcaggccca gcaacagctt
     1201 attgacagcc tgcgtgaggc gtttgccgat cgccaggtgg cggggctgct ttttggtaaa
     1261 gtgcagcgcg cccgggaact ggggctaaaa atgatcattg tccccggtag ccagctttcg
     1321 caactgccgc caggactgga tagcaccgag tttgcagcca ttgtgggcaa tttacttgat
     1381 aacgccttcg aagccagcct gcgtagcgat gaaggaaaca agatcgttga attattcctc
     1441 agcgatgaag gcgatgatgt ggtgattgaa gtcgccgatc agggctgcgg cgttccagag
     1501 tctctacgag acaaaatatt tgagcagggg gtcagtacgc gtgctgacga gcccggtgaa
     1561 catggcattg ggttgtactt gattgccagc tacgtaacgc gctgcggtgg tgttatcact
     1621 ctcgaagata atgatccctg cggtaccttg ttttcaatct atattccgaa agtgaaacct
     1681 aatgacagct ccattaaccc tattgatcgt tgaggacgaa acgccgctgg cagagatgca
     1741 tgcggaatat attcgtcaca ttcccggatt cagtcagata ttactggcgg gaaacctggc
     1801 gcaggcccga atgatgatcg agcgttttaa gccggggcta atcttgctcg ataactatct
     1861 tcctgacggc agagggatta atttattgca tgaactggtg caggcgcatt atcccggcga
     1921 cgtggtgttt accactgcag ccagcgatat ggaaacggtg tctgaagccg tacgttgtgg
     1981 tgtatttgat tatctcatta agcccattgc ctatgaacgg ctggggcaaa cgctaacccg
     2041 tttccgccag cgtaaacata tgctggaaag tattgatagc gccagccaga agcaaattga
     2101 tgagatgttt aatgcctatg ctcgcggtga acctaaagac gaactgccga ctggcattga
     2161 tcccttgacg ctaaacgcgg tgcgaaaact gtttaaagag cctggtgtgc aacatacggc
     2221 agaaacggtg gcgcaggcac tgaccatcag tcgcaccact gccaggcgtt atcttgaata
     2281 ttgcgccagc cgccatctga ttattgctga aattgttcac ggcaaagttg gcagaccgca
     2341 acgcatatac cacagtgggt gacatgataa agccggagga aacttgcctc cggcattact
     2401 atttacttgc ctgtgaccgc tgctgccgtt cctggcacca tcagctctgt agcaacgata
     2461 acaatcacca aaccaacaag caccggtacc gaggtgcgtt ttacgacttc aaacggcgag
     2521 atcttcgcca tcccggcaac cgcaacgact acgccagaaa cgggcgaaag ggtacggccc
     2581 aggtttgacg cctgcagcat cgggatagtc aaatacgccg ggttaatgcc ggaagagtgc
     2641 gccagtttcg ggatcatctc aacaaacgca taaaacggcg cattgcctga accggtcgtg
     2701 actgccgcca gcatggtcag aatcaccaat accagcatca ggatgatact cgccgaacca
     2761 aacgaggtag cgatagaaat cagactttga ataaagccga tggtgctaag cccctgagcg
     2821 aataccccag cggcaaccag cagcatcacc acgttagcaa acgcatctgc cattccgcga
     2881 taagccactt ccagaccaga gaaaactttc tgggtattaa agctgcggag gaactccaga
     2941 atggaggcaa tcagcataca aatcaccaga atagtgatga tgtgtaattg cggaccccat
     3001 ttaccgtcaa aaatcagtac accgatgatc ggcgtgaacg gcaaaatggc ataaaacgca
     3061 ggagcagtgg tggtgatttc actgacatct aacatttcat gagagatgtg ctctttttta
     3121 tccagataac gttgccagaa gaagtgggcg atcgccatgc cgataattgc agcaattgag
     3181 ataggcagcg tcgttttgaa ggcgaagtca atcagcgaca tttcggaagc ttgcgccgcc
     3241 agcaccacat cccctgaagt cggtgcgaga ataatcgccg ccggggaggc acaaatggca
     3301 gcagctgcgc cacgactgat accaacgttt accatcaccg gaaatagggt tgccatcagc
     3361 aaaacaccca gaccggttgc ggaagagacg gccagagaca tcagacaggc gacaaaatag
     3421 gcggcaatca tcagcaggta aggggagtta atatactgca atggttttga cgccagcttg
     3481 accaccatat cattcgcgcc gatatgggtc atgtaagcgg caaatccaca cagcatcata
     3541 atcatcatgc cgaggtcgcc gccgcggctc attagtaata ttttaacgta ttcaacgata
     3601 tccgtggcgc tgtagcctgt tgaagcctgg ctggacggta acactttgtg ccccataatg
     3661 gcactgataa tcagcaataa caggccaccg acaaataaca caccagtggc ggaataccct
     3721 ttaatgatgt agcgagctac acccacaata accacaaccc caataaggag ttcaatgaat
     3781 gtcagcataa tttttcctgt ctccaggccc caaagtaaat aataaaaaat tcctaaagct
     3841 taaggaaaaa atatgcccaa taaattggcg atgaatgctg attaaaatca agaaaaactg
     3901 ccattaagac attgaagttg ctgtttttat acatagataa caaaacgcat atttagcggt
     3961 tggcaattat cacataataa tgtgtgtaaa ttgatggtgg attgatgcaa atttgttaat
     4021 tgcaatggtg ttagctgcgg ctgtgcgctc aaaaaataat ctaacatgag cataggttga
     4081 cgatttat
//
LOCUS       DAPGWD010000247         4074 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPGWD010000247 DAPGWD010000000
VERSION     DAPGWD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.249"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            73..765
                     /locus_tag="RHX88_004305"
     CDS             73..765
                     /locus_tag="RHX88_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV2232909.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQK
                     TMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYA
                     KGEAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERI
                     IALNDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHF
                     RFKEDKQIKMTI"
     gene            complement(1023..3182)
                     /locus_tag="RHX88_004306"
     CDS             complement(1023..3182)
                     /locus_tag="RHX88_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDV2232910.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEAL
                     PKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGL
                     AALRVQLLKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAP
                     MPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHP
                     PLQRVYSSATFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVD
                     FTQGRLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNS
                     QLVISALTDKGLRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQP
                     DHLFRSLGFNRTWDEWRREEDARTHTTERRHDRGWSQ"
     gene            3361..3615
                     /locus_tag="RHX88_004307"
     CDS             3361..3615
                     /locus_tag="RHX88_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HDV2232911.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
BASE COUNT          977 a          981 c         1055 g         1061 t
ORIGIN      
        1 aaaaaaaaac attccacatt tcttatttgt ggggcacatt aaagcatcac cacatttgaa
       61 cgggatcttt gtatgtcgga cttgattgcc tacaagtcga atgctttggt tgaggccagc
      121 tacaagttga ccctacagga acaacgtttt ctgttgctgt gtatcagtcg attgaaatct
      181 ggtgctgatg ctgaattgca aaagacaatg accattaccg ctgcggaata ttttgattca
      241 ttccctgaca tggggcggaa aaatgccgag gttcagttac aggaagcgat cgatcgttta
      301 tgggacaggt cgatcattct caagaatgac gagaagcgtg aggagttccg ctggatccag
      361 tatcgggcac agtacgctaa aggtgaggcc aaggctcgaa taaccttttc tgacgcggta
      421 atgccttatc tgacgcaact gaaggggcaa ttcacgcgcg ttgttataaa aaacatatca
      481 ggtttatcaa gtagttacag tataagaata tacgagttcc tccagcaatt ccgcagtacg
      541 ggagagagaa ttattgctct taatgacttt cgttcaatgt tagggattga aaataaatac
      601 aaacaattca gagacttaaa taaaatactg ataaaaccct gtgttgacga actgaacaaa
      661 aaaagcgacc tggctgtaac ggttgagacg atcaaaaagg ggcgcacggt tgtagccctg
      721 cattttcgct tcaaggaaga caaacagatc aagatgacga tctaaaatcg attttaaggc
      781 acctaaaacg ctccctgctg cgtttttttt gtgttagtag tgcgaaggta ctattgaagt
      841 gaaaaattgc gtcaaaaacg ctctgatgca gcgaaaaaag aacccccaat ttttgggggc
      901 ggaaaaacaa aactatgctt gcattatgtg agtgtgcttc ttgcattcat ccctgtcgat
      961 gaacatcgaa gaacatcgac gaacataaca cgatgttaaa caaaacaaaa tattgttcac
     1021 attcactgtg accaaccgcg atcatgtctc ctctcggtgg tgtgtgttct tgcatcttcc
     1081 tcgcgccgcc attcgtccca ggtacgattg aaacccagcg agcggaacag atgatccggc
     1141 tgtttttctg gtgtgttgac ggcagcaaaa agcggtgcct cacgggtttt cagatgaaac
     1201 tccataagtt ccggcgcagg tgatatgtgg ctgtctgcat cgtgatttac ggcaacacca
     1261 taccctgcat accgtaaccc cttatcggtc agtgcactga tgaccagctg gctgttatcg
     1321 gctgagggaa cggcaaacgc gggatacagc gtaccattaa gccggacaac accgcccaga
     1381 gagtcatcca cgcccatgac cagcaaaagc tgaagctggc gggtttcccg gtcgacagaa
     1441 ctcattaccg gcatcagcgt cggtggcaga cgtccctgcg taaaatccac cttccttgct
     1501 gccagtgatg gcatggctgg ctggcggtct tctccaagac agaaagactc cggccatgtc
     1561 ttcggctggc ggagattgcg ggtatattgc ggccatgtct ggcaaacctg cgtcagaagt
     1621 tgctcaaagg tcgcagagga ataaacacgt tgcagtggtg gatgttgacc gatttcgctt
     1681 cggtcacaga tataaccact cctgtcacgc attaaatcaa cacgctcaaa gattctggtg
     1741 cggtatttct ccagctcgtt cccccagagc gtgtattctc cgttccggtt gtgaagcaga
     1801 aggtaacagg tcatttcccc gttaccaggc atgggggcct cgccataccc cgtaacgact
     1861 gcgctcagta cccctgaatc cataacggca ttgtcatttt tcctgagctg ttccgctcgt
     1921 cgctccgcgc gactacgttc tgtatcctcc tgctccttcc ggcgcaattc ctcttcacgt
     1981 ttaagcaact ggactctgag agctgcaagg ccggatgccc cccactgctg ccactgtttt
     2041 tcaaatgccg tctgactcgc gggaatgtcc tgtttctcca ccttccggta atgctgtcgc
     2101 aatttttcca tttgctggct cagcgactgg atttttccct gttcgctacg gtattccggt
     2161 gccgcccatt gcatcagggg aatacgggaa aaaccggacg aaaacggcga tgattttttc
     2221 aggctggcag catggctcat cgcttccttt cgttcggtgt tcagctcttc ccagcgtctg
     2281 cgcgtcgcct gcatatctgc catcatcgac atggcattac tggtgagttt ccatgccgca
     2341 ttactgtcct tcggcaacgc ttcgatgatg tcacgtagtg tgtacgaagt tgcgttgtca
     2401 ggaagcacga tattcagaag gtcagccaca cggcgggcag gctctgtcag cccgctgttc
     2461 ctgagttcag tgcgggaaat ctgtatttcc aggttaagcc tgttaatcag acgattctgt
     2521 tcgccgcgtt ctgtttcgat accgcgtgtt tccatcgcac aggcagactt tccgaggtga
     2581 ataccaggcg ttttctcaag cccctgtcgc tcgtatgaac gatggtctat ctcttcctga
     2641 taaccggcgt tcgccagtgc tctgtttgca tggtcagccc atgatgcacg ccacgtctcg
     2701 gcatgtgtcc ggtcgttcca gtccctgacc tttccgccga acccagcagg tccgacagcc
     2761 ctcgtggtca gcatgatgtg cgcatgggga tttgttttat ccatgtgatg gaacgccaca
     2821 tcagcaatca tgccctgact gacaaagttt tctctgacaa aggcgagaac ggtttcccgt
     2881 gcggcatcct gcggcagttc acgggggata gcgagttcaa tttcccgcgc aagttgtgag
     2941 ttcttccggc gttcggcctt ctcaacggca ttccagagtt cggcacggtt cgtacaccag
     3001 gcaggggctg tcgcgggggt cagaatcaca gcttcagcca caccgctttt gcgggtgtag
     3061 tcgtgagtca gtccggtacg ctcatcaagc attagtgaac cggaacgata ggcagcggaa
     3121 gctacggcgc tgtagccgct gcttctggag ataattttca tgctgagatg ataaatcgcc
     3181 atttctgccg taatgccttt ttgtagtttt ctttcagccc cgcagggcgc acacaatcga
     3241 aacggagtgg agattgtata agtgcgcact tcgtgatttg ccgtgcatta tgccagctca
     3301 gtttttaaac taccagtgac gcgctaaaat gcgatttttc atcgcgcacg gagttacatc
     3361 atgagcagaa cgctcgaaca gaagattgct gatgctgagg cgagattaca gcgcctgaag
     3421 gcgaagagcc ggagcctgga cacagcgcag aaggttgttg tgggtgcagc attgctggca
     3481 aaggtcagaa agccggagga ggtgcagttg cgtgcctggt tactccagtt cctgaaggca
     3541 gaggtgacac gacaggctga tgtgacgcgc atactgccgc tgattaacga gctggaggcg
     3601 ctgccaggac agtgattgta tcgtccgctc gccgcagccc cagcgaccga gcgttagcga
     3661 gcgagtgggc gaggaagcgg aagtgaatcc gtcgccaggt tgagcacata cgcacggttt
     3721 ttcggcaggt gatatgcgct gaactcgcat gaatactgga tttccacagg gttggaaggg
     3781 gagtattcgg aacttgcaat tctgctttct tgcaacacat tgtttttatt ggttttttgt
     3841 tttcagcacc ccaagttatc cacaggctcg ccgttaagtt tttagatctt gtttttaaga
     3901 tcttgttttt aatacttgtt ttaggggtga aattcattaa taaaatcaat atgttatagt
     3961 ggtatatgtg gaggaattta aggtaaaggt ggaggaattt aaggtgttta ggtggaggaa
     4021 tttaaggtaa aggtggagga atttaaggtg ttattcacag aaatgtgagg atat
//
LOCUS       DAPGWD010000248         4060 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPGWD010000248 DAPGWD010000000
VERSION     DAPGWD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.250"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>4060)
                     /gene="rhsD"
                     /locus_tag="RHX88_004308"
     CDS             complement(<1..>4060)
                     /gene="rhsD"
                     /locus_tag="RHX88_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HDV2232912.1"
                     /translation="PVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGI
                     FGPGWKAPSDIRLQIRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESLWLVRGGKATQ
                     PDGHTLARLWASLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYR
                     VLTGLADRFGRTLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQRTA
                     SLSSPDTPRPLSASAFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTY
                     TEAGELLAVYDRSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPA
                     GLSYRYLYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTRSGYDAAGRLT
                     AQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYD
                     EPGRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGY
                     QTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVI
                     TPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSVFSYDALDRL
                     VQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDG
                     HGWLREISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGL
                     ANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSMAGSNAAY
                     ELTSTYTPAGQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVR
                     TLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAKDAHYVYHYDEYGRLTE
                     KTDRIPAGVIRTDDERTHHYHYDSLHRLVHYIRIQYEEPLVESRYLYDPLGRRTGKRV
                     WRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDN
                     GEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQ
                     CGLTVEQLARQVEPEYTPARKAHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNE
                     ENPHHVYQPYRLPGQQHDEESGLYYNRHRYYDPLQGRYITQDPMGLKGGWNLYQYPLN
                     PLQQIDPMGLLQTWDDARSGACTGGVCGVLSRIIGPSKFDSTADAALDALKETQNRSL
                     CNDMEYSGIVCKDTNGKYFASKAETDNLRKESYPLKRKCPTGTDRVAAYHTHGADSHG
                     DYVDEFFSSSDKNLVRSKDNNLEAFYLATPDGR"
BASE COUNT          672 a         1340 c         1126 g          922 t
ORIGIN      
        1 cgtccatcag gtgttgcgag ataaaatgct tcaagattat tatctttact tcttacaaga
       61 tttttatcgc tacttgaaaa aaattcatca acataatcgc catgactatc tgcaccgtga
      121 gtatgataag cagcaactct atctgtacct gtgggacatt ttcttttcag aggatatgac
      181 tcctttctta aattatcagt ttctgcctta gatgcaaaat attttccatt agtatcttta
      241 cagacaatac cagagtattc catatcatta catagagatc tattctgcgt ttctttcaaa
      301 gcatctaacg cagcatctgc agtactatca aatttacttg gtcctattat acgtgaaaga
      361 acaccacaaa ctccccccgt acatgctcca gatctggcat catcccaagt ctgcaataat
      421 cccatagggt caatttgttg tagtggattt aaaggatact gatataaatt ccatccccct
      481 ttcaacccca tcgggtcctg agtaatatac cgcccctgca acggatcgta gtaccggtga
      541 cggttatagt acagccctga ttcctcatca tgctgctgcc ctggcagacg gtacggctga
      601 tacacatgat gcgggttctc ctcattaagc tggttgcccc attcatcata ttccgcgctc
      661 cacgccgtat tgccgtcttc gctgataagc gccagcggca gtccccggtg gtcgcagtga
      721 taaagatgag cttttcgcgc cggtgtgtat tccggctcca cctgtctggc cagttgctcc
      781 accgtcagcc cgcactgcgc aagccacgcc cggctttcac tgctcacgcg gtctgcccgg
      841 atttcttcct ccagtctgtc cagcagccgc accagttcag ccggaaatac cacgccgtgc
      901 ccgtcctcgc tcccttcctg ctgcagcttc tccgccaggc tgcggcgctg cgctttctcc
      961 cgctcgccgt tgtctgtttc gatgcggatg agcggcgcga agctccccgg ctgatatacc
     1021 gtctggatac gcgtggtgtc ggtctgtacc gtcgtcagcc ggtcgccgtc ccacccgtac
     1081 cacgtcacct ccggtttccg cgacagcgac atccatcccg tcaggtcacg ctcgcgacgc
     1141 cacacccgtt tccctgtccg gcgacccagc gggtcgtaaa ggtatcggct ctcgaccagc
     1201 ggctcctcat attggatacg gatgtaatgg accaggcggt gcaggctgtc gtaatggtag
     1261 tgatgggtgc gctcgtcgtc cgtccgtatc acacccgccg ggatgcggtc cgtcttctcc
     1321 gtcagtctgc cgtattcatc gtagtggtaa acatagtgcg catccttcgc gatgcggtta
     1381 tccggccaca ctgtgagggt actgtccggg tgcagctccg ggtccggcag ccggttgccc
     1441 gccgggtccg tggcatacgg gatgcggatg tccaggtccg gtgcgagggt gcgcacgctc
     1501 tccagcctgc ccgtggcact gtagccgtat tcccgcgtct gtcgcgggcc gctgatgcgc
     1561 accaggtcgc cgttgtcatt ccacccgtag tcacggtcat acaccaggct gttcaggtgc
     1621 tggctctgta actggcctgc gggggtgtat gtgctggtca gttcatatgc ggcattactg
     1681 cctgccatgc tgccaaagct gcgcaccgtc tcccggtgca gcctgtcccg cgtatactcg
     1741 accagcggcg tcccgcccag cttcatgccc gccaggtaac cgctgccgta ggtcagccac
     1801 tccaccggcg gcaggctgtc cggcgtgacg cggtttgcca gcccctgttc gttgtatgcg
     1861 tgtttcgtct catgctgcca cagcagttcc cccgtctccg ggttctccac cgtctgccgt
     1921 tcgcccgtca ggcggccctt atcgtcgtag ccgtagtgga cagcgacacg gtggccttcg
     1981 ctcaggtggc tgatttctct cagccagccg tggccgtcat actgccactg ctctgccggg
     2041 tcgccgttca ccgtccggtg cgtgatgcgg tccgacgcat cgtagtgcca gagggtgaca
     2101 agcccctcat cttcactctg cgtgagtttt ccggtcaggt cataatgata acgttgcgtc
     2161 cgcccgtcaa agccgccctg ctgtaccagc cggtccagcg catcgtaact gaagacgctg
     2221 tggctgccgt tctcgttggt caggctgatg acacgtccgg cagcatcgta ctccatactg
     2281 cgcgtcagcc cgccctgcgt ggtgctgacc gcctttcccc acgcatcgta ctgtgtctcg
     2341 ctccggttgc cgtccggggt gataacggca gtcaggtcgc ctgcggcgtt gtattcatac
     2401 cgcgtttcac ggccctgtgc gtctttcacc gaggttaacc ggccacgatt gtcatagcgg
     2461 cggtaaaggc tgatgccttc ctcgcggtgg accgccgtca tctggccgaa gcggtcgtat
     2521 tcataacggg tctggtagcc cgagcagtcg gtgaacgcca gcaactgccc gtagcggctc
     2581 caggtcatct gccgggtgct gcccgtcgca tctgttgtcg tggccggtaa ctcactgtgc
     2641 gcgtcatcgt agcggtagcg tactgtctcc ccgctgcgcg atgtctccga taccagcctg
     2701 cccggttcat catattcccg gcggctctcc agcccgtccg gggacaccac cgccgtcagc
     2761 tggttcccgt cgttatagta aaatttcgtc tcccgcccgt ccggtgtggt gatgtccgtg
     2821 atatcgccgg acaccacatt cagaccgtac tctgtccgcc gtcccgccgc gtccgtctgc
     2881 gccgtgagcc ttcctgccgc gtcatacccg ctgcgcgtga cgctgccgtc cgccagttct
     2941 tttttcacca cccgtttcag cccggccccg ccctctgtat gcagcacctc acgccggttc
     3001 aggctgtcgg tgacggtgat gcggtcctgc tcataaagat agcggtagct taaccctgcc
     3061 gggttcagtt gctccaccac ccgccccgta tcgtcgtagc ggtagcgcat ctccggcctt
     3121 cccgcgtaac ggtgcgccac catccggccc gggtgctgcg cgtcatacgt gaaagcgcgc
     3181 acctgcgtat tgctgcggtc atataccgcc agcagttcac cggcttccgt atacgtgtac
     3241 cgcgccagtg gcgcaccggg caggctttcc gggtatgccg ggtcgtgcat cagccacacc
     3301 gccgaaagac ggatgcccct gtcggggccg tattcggtac cgggcagtgt gtcggggaac
     3361 gctgaggctg agagagggcg gggtgtgtca ggggaagata aggaagcggt gcgctgttta
     3421 cgggcctctt ccgcacgctg cgcctgcgtg gtcagcacca gacggaactc ccgcccggca
     3481 ccgtccgtca cgccggtgat ttccccggcc aggtcaccgg cggcctcacg ccggtacgtc
     3541 agcgtccgcc cgaagcggtc tgccagcccg gtaagcaccc ggtacggcgg cagcggcgct
     3601 ggcagtacgt cctcagcacc cggcacccgc tctgaccacc ccagtatcca ccacggcccc
     3661 tgtgcgctgt tggtcgccag gtaaagatgc gggcttaacc ggatatccgg cggcagcgac
     3721 gcccacagcc gtgccagcgt atggccgtcc ggctgcgttg ccttaccgcc acgcaccagc
     3781 cacagggact cgctgcggct gtacaccgcc tcacccggca gcagcggctc aaagtgaatg
     3841 ctccgtccgc cgttgtcgtt gagtaccagt gcatcatcgc gtatctgtaa gcggatatca
     3901 gaaggcgctt tccagccggg gccaaaaatc cccaccggtg caggcgtctt cgtccggtag
     3961 ctgctgtagg tgcgggagag aatgaacggc agcgggccgg gcagcgcaag gtccgtctcg
     4021 ccgggcagca ccttcgcccc cagcagcgga tttaccgggt
//
LOCUS       DAPGWD010000249         4056 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPGWD010000249 DAPGWD010000000
VERSION     DAPGWD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.251"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..271
                     /gene="cbtA"
                     /locus_tag="RHX88_004309"
     CDS             <1..271
                     /gene="cbtA"
                     /locus_tag="RHX88_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDV2232913.1"
                     /translation="LVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELML
                     TSGELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN"
     gene            360..733
                     /gene="cbtA"
                     /locus_tag="RHX88_004310"
                     /pseudo
     CDS             360..733
                     /gene="cbtA"
                     /locus_tag="RHX88_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
     gene            730..924
                     /locus_tag="RHX88_004311"
     CDS             730..924
                     /locus_tag="RHX88_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232914.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAEKGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            937..1050
                     /locus_tag="RHX88_004312"
     CDS             937..1050
                     /locus_tag="RHX88_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDV2232915.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(1339..1485)
                     /locus_tag="RHX88_004313"
                     /pseudo
     CDS             complement(1339..1485)
                     /locus_tag="RHX88_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1539..1721
                     /locus_tag="RHX88_004314"
     CDS             1539..1721
                     /locus_tag="RHX88_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDV2232916.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(1822..2151)
                     /locus_tag="RHX88_004315"
     CDS             complement(1822..2151)
                     /locus_tag="RHX88_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDV2232917.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2323..3381)
                     /locus_tag="RHX88_004316"
     CDS             complement(2323..3381)
                     /locus_tag="RHX88_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV2232918.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEINTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(3579..4052)
                     /gene="sbmC"
                     /locus_tag="RHX88_004317"
     CDS             complement(3579..4052)
                     /gene="sbmC"
                     /locus_tag="RHX88_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDV2232919.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
BASE COUNT         1080 a         1020 c          952 g         1004 t
ORIGIN      
        1 tctggtgcag gagggtaacc ggttgcatta ccttgccgac cgcgccggta tcagaggcct
       61 gttcagcgat gcagatgcgt accacctgga ccaggccttt ccgctgctga tgaaacaact
      121 ggaactcatg ctcaccagcg gtgaactgaa tccccgccat cagcataccg tcacgctgta
      181 tgcaaaaggg ctgacctgca aagccgatac cctcagcagt tgtggttacg tttatctggc
      241 tgtttatccg acgcccgaaa tgaaaaatta actctccaga atagccttct gctacggcct
      301 ggtgttttca ccacgccact tttccatttt tatatctgca tatcaggaaa atcttcagta
      361 tgaaaacatt acctgtatta cccgggcagg cgaccagttc tcgcccgtct cctgttgaaa
      421 tctggcagat actgctgtcc cgactgctgg accagcacta tggcctcaca ctgaatgaca
      481 caccttttgc cgatgaacgt gtgattgagc agcatattga ggcaggcatt tcactgtgtg
      541 atgcggtgaa ctttctcgtg gaaaaatacg cgctggtgct accgaccagc cgggattcag
      601 cgcctgtacc cgctctcagt taataaacag catcgatatc ctccgggctc gcagggcgac
      661 cggcctgatg acccgcgaca attacagaac ggtaaataac attaccctgg gtaagtatcc
      721 ggaggcgaaa tgatgacact ggaagccgac agcgttaacg tacaggcgct ggatatgggg
      781 cacattgtcg ttgacattga tggtgttaat atcactgaac tgattaataa ggccgctgaa
      841 aaaggttatt cactccgcgt ggtggatgac cgtgactcta ccgaaacacc ggcaacttat
      901 gccagccctc accagttgct gtaagacaat gcagtgatgc agggtatagt gccattaacc
      961 acagaaacag cactgttgtc ctgtattaag caggcaagag aagatgtcct taccctgcgc
     1021 catctgcagc ttctgcacca gaaccggtga atctcactca acaggcttca tttcccctga
     1081 cgccagcctg aatacagctg gcgttttcat ttataaacag aaaggaaaac cgataataat
     1141 ggaatggacg ccccgaccat gaagcaacgg taggatatta ctgataaacc ttccagtgga
     1201 ggtgcgttat ggccgtttta acgattagca ttgacctgac aaaaaatgaa ttccaaatcc
     1261 acggtctggg taggaacaga aaaatctaag ctcagaaaac ggattaaagt aagcgtcaac
     1321 ggagcaccgt attgacgctt atttattggt gagaactacg ttccatggca ggagttcgtc
     1381 aacacggttg gagggccatt ccggcagtat gctcagaata tggcgcagat acgcttccgg
     1441 atcgataccg ttcagacggc aggtgcctgc ttttccggca acaagcagga atctcagcgc
     1501 tgaagcagga attagaggta aaaacgccct accgggccat gaatcacccg gtcattggag
     1561 tagtaaccaa agcagattta gccagcatgg aacaaatatc tttggtgaaa agttggttac
     1621 gggaagctgg agcgcacaac gtgttagtaa ccagtgcagt taataacaat ggagttacag
     1681 aactttttgc cttgctgcat acagaagaag gctgttgtta atttcgattg gttctcagat
     1741 gagaatgatg acggtgatac acagataaga atcaatgagc aatgagggtt gccgggcaac
     1801 cctcattgaa taaaacggga attacttcgc ttcgccgttt ttcatttcgc ccatagcttt
     1861 cagctttttg gagatatcgc ggcgttcttt ggagagctcg gcatttttga tgatgtaatc
     1921 gtcaacgcga tcttcatagt cacctttcat gctggcgatg atgccctgga ttgcttcaac
     1981 gctcatccct ggcttgatgt aatcgctcag gttgtccagc aacaggacgc gcttctggtt
     2041 gtcacggatc tttttctcaa cgtcctgaat ttcacgttgc agtttgttct tacgacggaa
     2101 cagacgaaca aattccagta cgtcctggaa tgaaggcttg gtagtttcca tttttatacc
     2161 cctgataatg tgagagtcgg attcgtttaa gcaaccgcta ttcgttaggg ccaacattac
     2221 tcatagctgc cgcagatgac aatgctttta tcccttttca ctatcatacc ctttatcctt
     2281 gctgaatcga agcagcagca agatgattct gaagttcagg aattatttgc gcaaggcccg
     2341 gcaaatcaga ttcgatagca actcaagctg atgtgccgtt tccatgttca gccacacata
     2401 accatagatt ggtgtttcca caaccttcag gtcatggtgg ttattcagca actgacgcag
     2461 ctcttccaca gcatcgttca atttttccgt attggcaaaa accggctgcg gattaccttc
     2521 gtacagcgca tgaacaaggc tcagcagtat ttgctgcatc atatgctggg tatcacgaag
     2581 tttttgcgcg ttcaataaca cgaaatggct ggggcgcgtg gcccagtatg cattgatttg
     2641 caactccagc atacaaacca gattgcgatt aatggtctgg ataccttcat atatcgattt
     2701 tggaatacgg gtttctttgc tggcgggcgc aatcagccca cgcattttca cggcatcggt
     2761 caggagtttt tgtagatggc tttccagacg cgggcgttcg agtaagttcg gtgagaatgc
     2821 agattgatag acccgattat actcggtcag actttttgcc agttgaatgc gccagtggat
     2881 gaacgcccgt tgtggccaga taccggtaaa caacattgcc agtaaagagc cgaggatcac
     2941 atcgccgctt cgccataacg ccgtattaat ttcacctgtc ggggaaccaa caacaattgc
     3001 cagcgtcacc ccaatcaata aaccttgata cggtttcttg cccagcgcca gccaaccgca
     3061 aagaaacata gccgccgcgc accagactaa catcagcggt aacgagatta actccagttg
     3121 cagagcgata agacctaaaa tcgaccccaa caccgtaccg ccaatacgtt cgaatgcgcg
     3181 agggacaacg ttcccccaga acgagattgg ccccataatc accaccatgg tgaccagcgg
     3241 ccaggtgctt tccgggatag taaacaggcg gataatgaga aaagtgagca ggaatgccag
     3301 cgcgacccga gtaccatgca caatgcggta atggcggtat acccggattt caaacgggct
     3361 taatgactta tcggcacgca cgcagaaact ccgtaatgaa gattaaaagc gctcataact
     3421 catctgtcgg ccagaaggcg cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta
     3481 atttcccggc aggagcgaaa aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa
     3541 agagtggtct tcgcgtcaac acaccgccct gagatgaatt agtgatgttt tggctgcacc
     3601 gcaacataca tttcgatatc ccagtaccca tcttccgcgc cattgttcaa gtaaacctcg
     3661 aagcagggct ttggtaacat ttcataagca ctgtcctgca agaggctatt aaagaactga
     3721 taccagggtt tagcaaaatc atcaccgact acacgagcca ccgccaccgc atattgacca
     3781 cctgaaattt ctgtcagaat gacgccctca ctgttttcgg ggagcgtaaa gttattcggt
     3841 accgtcacga cggtgtcgca gcgtaatttt tcggcgggtg tttcatccgg attgtcgtaa
     3901 tagacagcaa cccactcctt cggcacaata tttttgctat ctacccacat catcaactgc
     3961 tcaaaacctt tctttaccgt ctgttcccac gggccaacga gatggaaacc tgcaacggta
     4021 cgtttatctt cctgcttaat ctcgtagttc atgacg
//
LOCUS       DAPGWD010000250         4001 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPGWD010000250 DAPGWD010000000
VERSION     DAPGWD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.252"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..216)
                     /locus_tag="RHX88_004318"
     CDS             complement(<1..216)
                     /locus_tag="RHX88_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV2232920.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDII"
     gene            complement(230..2710)
                     /locus_tag="RHX88_004319"
     CDS             complement(230..2710)
                     /locus_tag="RHX88_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2232921.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQTYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(2726..3400)
                     /locus_tag="RHX88_004320"
     CDS             complement(2726..3400)
                     /locus_tag="RHX88_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2232922.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(3480..>4001)
                     /locus_tag="RHX88_004321"
     CDS             complement(3480..>4001)
                     /locus_tag="RHX88_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2232923.1"
                     /translation="AAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVS
                     INMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKA
                     IASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSG
                     DVLSSLVMRVAQD"
BASE COUNT         1119 a          983 c          745 g         1154 t
ORIGIN      
        1 aataatatcc gacgggctca taggggtgat agtatttgta ctcacgcatg acgaatcatt
       61 aaaattatgc tcatatgtcg ccatttgtgt ttgagtaagt gcaaaatccc cctgaacaac
      121 caaattgtgc tgaacagata actcactata acatgccgca tagctcacag gcataaccat
      181 catcattact ataaataaca ttatacgaat attcataacc acctgactat tactggcaaa
      241 tatagtttct actttcatca atctctttac cgaaggtgat tgtgcatgaa agtccttgtt
      301 gcttatcaat tgctacatta accgatggcg gtacttcatt ggtgcgaata aataactggc
      361 ttccctgacc gacaacgcca atattatgac catggatatc attgacttca taaccaaaca
      421 ttaatggttg cccatccgct cttaacgctt ttataaacca gggcttgcgc tgatcggtat
      481 caaaattaac cagtacaacc gcgccgcgat aaggggcggc aattttccgg ttgccacgta
      541 attctgcttc gctatcgctt tgcgacacat ccagcatcag gtgattttcc cgataaggtg
      601 tcattccgtc gtatatcacc actccattac ggtttgttgt gcgatatttt tgcccattga
      661 cataagcatc tttaattcct ggcgcattca tcacagcaaa cgtttcggaa agacggttcg
      721 ccagattaac gccacccgac caggcgacaa tgccccctga aacactggct ccagcctgtc
      781 gataagtact cgactgacta taactgccat tcactgtcgc aaccggcgcg ttccaggtca
      841 aattcgcccc agctgtcgtt tcatttccct gatgctgata actcaggttg accccatagt
      901 taaactgatc ccggcttcca acggttcctg ataatcccgt attatttgag gcaaacccct
      961 gatcatcaaa cgtcgttgag ttagacatat atatttgccg acgaggcgtc gtaacgtcat
     1021 caccccaatc aaagggaatc gatataaaaa tattaaaacg tttctcttca tgatgattct
     1081 cgtcatacgt ctggcttgcc gcgaggatat agcttatccg ccgccagtta ttggaataac
     1141 tcaactgata atccttactg ctgccgctac gcccccagta atctcgccat aacgtactta
     1201 aggacacaga cccccagcct tctggcaatg actggctcat attggcagag aagctatttt
     1261 tacggccaaa atcgttctgg taataatcgg caatgtcata gacatcgttt tcatcacggc
     1321 gataattatc tttattgttt gcccaaacgt gatcattaaa tgtccggtaa tcacgcgatg
     1381 aataacgcca ggccgccaga ccaaaacgcg tcgacgtttg gctcacaaat ttgttgtagg
     1441 caatttgata actttgcccg tcaaacacat cgccgttgtc ttgtttacta tgcgacttcg
     1501 tggcatcgac ggaaatggca ccaatgcgtg tattccagcc agtccccaaa gtaaacgcgt
     1561 aataattatt cgcgaccatc gagccaccat acagcgtcaa taaattatta aaaccatact
     1621 gataacccgc ctggacaaaa tcactttgtt tgctcgcccc ttcaatatgg ctacgacccg
     1681 ccgcaaaatc atatttcgac acgccgggtt gcagcatatt tggcaccgct gcataaggca
     1741 ccagataggt ggttaccgaa ccgtccgcct ctttcacgct gacatcaaga tctgctccac
     1801 caccggccaa ctgcaaatct gtaatcgcaa acgggccagg aggaacctct ttctgataaa
     1861 ccacaaaacc attctgttca atagttacca gcgcgttact ctgagcaatc ccctgcaccc
     1921 gtggcgtaaa attttgtttc gagttaggca acatctgcat atcacgaaac aaccgcacac
     1981 cactgaagcg aacagaatca aaaatatcgc ttgatgtgta catatcaccc acgcgaagcg
     2041 tgccgagaag ttgggcaaat ccacgttcca gatacagggt attgcttttc cacacccctg
     2101 gattgttatt tgttttactg aaactggcat cagaatgcag ttgccactcc agtaaattta
     2161 acccgctgtt aaaacgtaca tatgtactct tgttgttacc cgacgcttta tagtcgctgt
     2221 aatactgact cacataataa gaggtataaa acgcattaat accccgctcc cagttttccg
     2281 gtggaacata gccactttcc agttcttcca cccaggcctg cgggacactg aaatcgagac
     2341 gaaaaacccc gatatcccag gtatagctcc caccctgaac aagttgctca aatgttaaac
     2401 attgcttacc gctggcgaag ttatcgctat taatgcctaa ccgcttgata acttctcttg
     2461 ataaacatgt ttcttgcggg ttgtctttaa caataatctc atatttcccg cgccattgct
     2521 tattgacata aatatcaatg tcatactgcc ccggtaaggg ttgattatca tcaagacgaa
     2581 tatttgcaac ctgctggtct ttcattccac ctatcataaa atgggtatca aaggtttctt
     2641 cagctgcata agcttcaatg ccgagcaata acgctacgat tgctgatgca agtggggtca
     2701 ttctcaacat aggcagctcc tgcaattaaa ttttgtcact aatatagttg ccgtggtcat
     2761 cgataatggt cagataccag ttatttgcat tattactttt gacattaaca ctctgacttt
     2821 ctaagggggc aatcattata gtttcataat tgactttgac attattagct ttgacatccg
     2881 aaatcgtcac ccaattagct gagtcatttt ttatcaccaa tccattgcca ctgcgattta
     2941 ccagtaactt tttaaaggtc gctttattta ccggagcaat acccgccggc cggtaaaaca
     3001 atttgattct gttttgcatc gcaaacttca gtgcattctt accttcttgt tctggactat
     3061 ttggtggaat atccagaaca ttcagataaa aaatgctttc tttattagtg ggcagtttat
     3121 tcggcataat tttgattttt acttgctgcc cggaatttgc ccctattttt gcaactggtg
     3181 gcgttaacat gaaaggaacc tgaatttttt ctggtggtaa cgacgtatcg ccatcatcaa
     3241 tccacgcctg cagcagcgaa gaacgatttc cctggttcat caactgcacc ataacttctt
     3301 tattttctgc cgggtaaata atgcgcgtcc cgtagataac aattccggca tgtgcaggaa
     3361 ggaccataga aaaaatgagt aaagataata atcctttcat tttaatgcct cttaaattaa
     3421 aaggccgcca tgggatagcg gccatgttta ttacctggag aaacttgcaa ttttatttat
     3481 taatcctgcg ccacacgcat aaccagagaa gataacacat cacctgattg cactgagtta
     3541 tctgttagtt tcaggtattt tgctttaaac ggaatagcat attgaccttc cgctgtttta
     3601 taactctcaa tattaaaagg tgcagaaaca tccaggacat cgctgttatc acctaaatag
     3661 agatagtaac ccacggtatc gttgctactg gctatcgcct tatcgttgcc ggttattttg
     3721 tcgttggtaa tcaacgagag ttttacacca gcaacttctt tcgggcagct aatttttaac
     3781 atgctgctgg ttacaccgac tgcatcatca taggtagaat atttagcttt accttcaaag
     3841 acagtggttg gtacagtcgg catattaata cttactgaat caccacccgc ttcgaattta
     3901 cacggagatt ctgcaatatt tcctttaatg gttaatgttc cggtatcaac gtctgcagca
     3961 aatactccag cgctcataaa aaaagaagaa aagacagcgg c
//
LOCUS       DAPGWD010000251         3996 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPGWD010000251 DAPGWD010000000
VERSION     DAPGWD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.253"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            84..692
                     /locus_tag="RHX88_004322"
     CDS             84..692
                     /locus_tag="RHX88_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDV2232924.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            790..2181
                     /gene="selA"
                     /locus_tag="RHX88_004323"
     CDS             790..2181
                     /gene="selA"
                     /locus_tag="RHX88_004323"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDV2232925.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2178..>3996
                     /gene="selB"
                     /locus_tag="RHX88_004324"
     CDS             2178..>3996
                     /gene="selB"
                     /locus_tag="RHX88_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDV2232926.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQFNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
BASE COUNT          879 a         1017 c         1201 g          899 t
ORIGIN      
        1 gaagtaacaa accatcaaca ttttctgccc tttttcccat caccaggcca tactgatatt
       61 tttcctcgca caaaggactc ggcatgaaac tcgtcggtag ctacaccagc ccgtttgtac
      121 gcaaactttc tattctgttg ttagaaaagg gcattacttt cgaatttatt aatgaactgc
      181 cctataacgc ggacaacggc gtggcgcaat ttaacccgtt aggaaaagtg ccggtgctgg
      241 tgaccgaaga gggcgaatgc tggtttgatt cgccgatcat cgctgaatat attgaattaa
      301 tgaatgtcgc tccggcgatg ttgccgcgcg atccgctgga gtcgttgcgg gtgcgcaaaa
      361 ttgaggcact ggcggatggc attatggatg ctgggctggt atcggtgcgt gaacaggcgc
      421 gtccagcggc gcagcagtct gaagatgaat tgttacgcca gcgggagaaa atcaaccgca
      481 gtctggatgt gctggaagga tatctggtcg atggcacact caaaaccgat acggtcaatc
      541 tggcgactat cgccattgcc tgtgccgtcg ggtatctcaa tttccgccgt gttgcgccag
      601 gctggtgtgt cgaccgccca cacttagtca aactggtcga aaacctgttt agccgcgaaa
      661 gttttgctcg caccgaaccg ccaaaggctt gatgcgcgat atgtcctcct gacccatctc
      721 acgttacaat ccgtggttat gttaaacgcc cttctccgtg tgagagggcc ttgatcagcc
      781 aggtttccta tgacaaccga aacgcgttct ctctatagtc aacttccggc tattgatcgc
      841 ttattgcgcg atagctcctt cctttctttg cgtgatactt atggtcacac ccgcgtggtg
      901 gaattgttgc gtcagatgct cgacgaagcg cgagaagtga ttcgtggcag ccagacgctg
      961 cctgcgtggt gtgaaaactg ggcgcaagaa gtcgatgccc ggttgacgaa agaggcgcag
     1021 agcgcgctgc gtccggtgat aaacctgacg ggaaccgtgc tgcataccaa tcttgggcga
     1081 gctttacagg cggaagctgc ggtggaagcc gttgcgcagg ctatgcgttc gccagtgacc
     1141 ctcgagtacg atttggacga cgccggacgc ggacatcgcg atcgggcgct ggcgcagctg
     1201 ctgtgccgta ttacgggggc ggaagatgcc tgtatcgtta ataacaatgc ggcggcggtg
     1261 ttattgatgt tggcggccac tgccagcggg aaagaggtag tggtatctcg cggcgaactg
     1321 gtggagattg gcggcgcgtt tcgtattccc gatgttatgc gtcaggcagg ctgcacccta
     1381 cacgaagtag ggaccaccaa ccgcacgcac gcgaatgatt atcgtcaggc ggtgaatgaa
     1441 aataccgcac tgttgatgaa agtacatacc agtaactaca gcattcaggg gttcaccaaa
     1501 gcgatagacg aagctgaact ggtggcgctc ggcaaagagc tggatgttcc cgtagtgact
     1561 gatttaggca gtggctcgct ggtcgatctt agccagtacg gtttgccgaa agagccaatg
     1621 ccgcaggagt tgattgcggc gggcgtcagt ctggtgagct tctccggcga caagttgtta
     1681 ggcgggccgc aggcaggaat tattgttggt aaaaaagaga tgatcgcccg cctgcaaagc
     1741 cacccgctga agcgtgcatt acgcgcggat aaaatgaccc tcgcggcgtt ggaagccacg
     1801 ttgcgtcttt atttacaccc tgaagctctg agtgaaaaat taccgaccct gcgcctgctt
     1861 acccgcagcg cagaggtcat tcaaatccag gcacaacgtt tacaggctcc ccttgccgca
     1921 cattacggcg cggagtttgc ggtacaggtt atgccatgtc tttcgcagat tggcagtggt
     1981 tcgctgccgg ttgatcgcct gccgagcgcg gcattaacgt ttacacccca tgatggacgc
     2041 ggtagccacc ttgagtcatt agccgcccgc tggcgtgaat tgccagtgcc ggtgattggt
     2101 cgtatttatg acggacgatt gtggctggat ttacgctgcc ttgaagatga gcaacggttt
     2161 ttggagatgt tgttgaaatg attattgcga ctgccggaca cgttgaccac ggcaaaacaa
     2221 ccttattgca ggcgattact ggcgtaaatg ccgatcgtct gccggaagaa aaaaagcgcg
     2281 gcatgactat cgatctcggc tatgcctact ggccgcagcc ggatggccgc gtgcctggtt
     2341 ttatcgacgt acccggtcat gaaaagtttc tttccaacat gctggcgggc gttggtggta
     2401 tcgatcacgc gctgttggtg gtggcgtgcg atgacggcgt gatggcacag acccgtgaac
     2461 atctggcgat tttgcagctg accggtaacc cgatgctgac agtggcgctg accaaagccg
     2521 atcgcgtgga cgaagcgcgt gttgatgagg ttgaacgcca ggtaaaggag gtgctgcggg
     2581 aatacggttt tgctgaggca aaactgttta tcaccgcagc aaccgaaggt cggggaattg
     2641 atgccctgcg cgagcatctg cttcagttac cagaacgtga acacgccagc caacatagtt
     2701 tccgcctcgc gatcgaccgc gcatttaccg taaaaggtgc cgggctggtg gtcaccggta
     2761 cggcgttaag cggggaagtg aaggtaggcg attcactatg gctgactggt gtaaataaac
     2821 cgatgcgcgt gcgtgcgctg catgcgcaaa accagccaac tgaaaccgcc catgccgggc
     2881 agcgtatcgc gcttaacatc gcgggtgatg cggaaaagga gcagattaac cgtggcgact
     2941 ggctgcttgc cgatgtgccg ccagagccgt tcacacgggt gattgtcgag cttcaaactc
     3001 atacaccgct gacccagtgg cagccgctgc atattcacca cgccgccagc catgttacgg
     3061 gacgcgtttc gctgctggaa gataaccttg ccgagctggt gttcgacacg ccgctgtggc
     3121 tggcggataa cgatcgctta gtgctgcgcg atatctcggc ccgcaacacg ctggcggggg
     3181 cgcgtgtggt gatgcttaac ccgccgcgtc gcggtaaacg taagccggaa tatctgcaat
     3241 ggctggcgtc actggcacgg gcgcagagcg atgccgatgc gttatctgtt catctggaac
     3301 gcggcgcggt taaccttgcc gatttcgcct gggcacggca gttcaacggc gaagggatgc
     3361 gcgaactatt gcaacagcct ggctatattc aggctggtta tagcttgttg aatgcgccgg
     3421 ttgccgcccg ctggcagcgg aaaattctcg acacattagc gacttatcat gagcaacatc
     3481 gtgatgaacc tggtcctggg cgcgaacgtc tgcgacgtat ggcgttgcca atggaagatg
     3541 aagcgctggt actgttgctg attgaaaaga tgcgcgaaag cggcgacatc ctcagtcatc
     3601 acggttggct gcatctgccg gatcacaaag cgggcttcag cgaagagcag caggccatct
     3661 ggcaaaaagc agagccgctg tttggtgacg agccgtggtg ggtgcgtgac ctggcaaaag
     3721 agacgggaac cgacgagcag gcaatgcgtc tgacgctacg ccaggcggcc caacagggga
     3781 taattaccgc gatcgtcaaa gatcgttatt accgtaacga tcggattgtc gagtttgcca
     3841 atatgatccg cgatctcgat caggagtgtg gttcaacctg cgcggcggat ttccgcgatc
     3901 gcttaggcgt aggccgaaag ctggcaattc agattctgga atattttgac cgcattggct
     3961 ttacgcgtcg tcgtggaaat gatcatttat tgcgcg
//
LOCUS       DAPGWD010000252         3752 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPGWD010000252 DAPGWD010000000
VERSION     DAPGWD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.254"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            82..2361
                     /gene="ydeP"
                     /locus_tag="RHX88_004325"
     CDS             82..2361
                     /gene="ydeP"
                     /locus_tag="RHX88_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDV2232927.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2609..2806
                     /gene="safA"
                     /locus_tag="RHX88_004326"
     CDS             2609..2806
                     /gene="safA"
                     /locus_tag="RHX88_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDV2232928.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            2881..3642
                     /gene="ydeO"
                     /locus_tag="RHX88_004327"
     CDS             2881..3642
                     /gene="ydeO"
                     /locus_tag="RHX88_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDV2232929.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMNALAI"
BASE COUNT         1046 a          831 c          868 g         1007 t
ORIGIN      
        1 gttggcgata atccctttgt atgtactgtg catcatcgct attacaaatc ctaataattc
       61 atttccacac aggataagta gatgaagaaa aaaattgaat cctaccaggg tgctgcaggt
      121 ggttggggtg ctgttaaatc cgtagcgaat gcagtacgta agcagatgga tatacgccag
      181 gatgttattg ccatgtttga catgaataag ccagagggct ttgactgtcc gggttgtgca
      241 tggccagatc ctaagcacag tgcgtcattc gacatttgtg aaaacggcgc aaaagcaatc
      301 gcctgggaag tcacggataa gcaggtaaat gcctctttct ttgctgagaa tacggttcaa
      361 tcattactta cctggggaga tcacgagctt gaggctgcgg gacgtctcac tcagcctttg
      421 aaatatgatg ccgtcagcga ctgttacaag ccattaagct ggcaacaagc tttcgacgaa
      481 attggcgcac gccttcaaag ctatagtgat cccaatcagg ttgaattcta tacttcgggc
      541 cgcacttcca atgaagctgc ctttctttat cagctttttg cccgtgaata cgggagcaat
      601 aactttcccg actgctccaa catgtgccat gaaccgacca gtgtgggttt ggcagcgagt
      661 atcggcgtgg gtaaagggac tgtgttgctg gaagactttg agaagtgcga tttagtcatt
      721 tgcattgggc ataaccctgg tacaaaccac cctcgcatgc tgacttcgtt gcgcgcttta
      781 gtgaaacggg gagcgaaaat gatcgccatc aatcctctac aggaacgtgg cctggagcga
      841 tttaccgcac cgcaaaaccc gtttgaaatg ctgacgaact ctgagactca gttggccagt
      901 gcctactaca acgtgcgcat tggtggcgat atggcgttgc tcaaggggat gatgcgcctg
      961 ttaattgagc gcgatgatgc tgcaagtgcc gcaggtcggc cctcattgct tgatgacgaa
     1021 tttattcaaa cgcataccgt cggctttgac gagctacgcc gtgacgttct caattccgag
     1081 tggaaagata tcgaacgtat ttctggacta agtcagacac aaatcgccga actggctgac
     1141 gcatatgctg ctgccgaacg caccattatc tgttacggaa tggggatcac tcagcacgaa
     1201 catggtaccc agaacgtaca gcaactggtc aatttgctgt tgatgaaagg taacattggc
     1261 aagcctggtg cgggtatctg cccactacgt ggtcactcta atgtacaggg cgaccgaacc
     1321 gtcggtatca ccgagaaacc gtctgcagag tttctggctc gcctgggtga gcgctatggc
     1381 ttcaccccac ctcatgcacc tggacatgct gcaattgcca gcatgcaagc aatatgtacg
     1441 gggcaggctc gagcattgat ctgcatgggg ggcaactttg cactggcaat gccagatcgg
     1501 gaagcgagcg ctgtaccgtt aacgcaatta gatttggcgg tacacgtagc cactaagctt
     1561 aaccgctctc atctgctgac cgcacggcat agctatattc tgccggttct ggggcgtagc
     1621 gagattgata tgcaaaaaag cggtgcgcag gcggtaaccg ttgaggattc aatgtcgatg
     1681 attcatgcct cgcgtggcgt gttaaaaccc gccggtgtaa tgctgaaatc agagtgtgct
     1741 gtggtcgcgg gaatcgccca ggcaacacta ccccagagcg tggtagcctg ggagtatctg
     1801 gtggaagatt atgatcgcat tcgcaatgac attgaagctg tgctgccaga gttcgccgac
     1861 tataaccagc gcatccgtca tcccggtggt tttcacctga taaatgcagc tgctgaaagg
     1921 cgctggatga cgccgtcagg taaggctaat ttcattacca gcaaagggct gttagaagat
     1981 ccctcttcag cgtttaacag taagctggtc atggcgacag tacgcagcca cgatcagtac
     2041 aacacgacga tttatggtat ggatgatcgc tatcgagggg tattcggtca acgagatgtg
     2101 gtctttatga gtgctaaaca agctaaaatt tgccgtgtaa aaaacggcga aagagttaat
     2161 cttattgcgc ttacgccaga cggtaagcgc agctcacgcc gcatggatag attaaaagtg
     2221 gtcatttacc ctatggctga ccgctcactg gtgacctatt ttccagaatc gaatcacatg
     2281 ctaactcttg ataaccacga tccattaagt ggcattcctg gttataaaag tattccggtt
     2341 gaattagaac catcaaatta attcgcctcc tcatttcttc tgctgtcatc cgcacagcag
     2401 aagaatttct cattgactat tatcgcgcaa tttgctcaca ttgattaaat taaaccacac
     2461 actataagat ataaacctct gcctacagct gtaagaaact ccgctcagta ctgaagtgcc
     2521 agtcctattt cctcttttct ccagcctgtt atattaagca tactgattaa cgatttttaa
     2581 cgttatccgc taaataaaca tatttgaaat gcatgcgacc acagtgaaaa acaaaatcac
     2641 gcaaagagac aactataaag aaatcatgtc tgtaattgtg gttgtcttat tactgaccct
     2701 tacgttgata gccatttttt cggcaattga tcagctgggt atttcagaaa tgggtcgcat
     2761 tgcaagagat cttacacatt tcattatcaa tagtttgcaa gactgaaaat aaacagcaaa
     2821 ttataaatat gaaatgttaa aaaagtatcg ataaaaactt tattgtttta aggagataaa
     2881 atgtcgctcg tttgttctgt tatatttatt catcatgcct tcaacgctaa cattttagat
     2941 aaagattacg ccttctctga cggcgagatc ctgatggtag ataacgctgt tcgtacgcat
     3001 tttgaacctt atgagcggca ttttaaagag atcggattta ctgaaaatac cattaaaaaa
     3061 tatctacaat gcactaacat ccagacagtg acggtgcctg ttcctgcgaa gtttttacgt
     3121 gcttcaaatg taccgactgg attgcttaat gaaatgattg cttatctcaa ctcggaagaa
     3181 cgcaatcatc ataatttttc agaacttttg cttttttctt gcctgtctat ttttgccgca
     3241 tgcaaaggtt tcattacact attaactaac ggtgtgctat ccgtttccgg aaaagtgaga
     3301 aatattgtca acatgaagct ggcgcaccca tggaagctga aagatatttg tgactgcctg
     3361 tacatcagtg aaagcctgtt gaagaaaaaa ctaaagcaag agcaaacgac attctcacag
     3421 attctattag atgccagaat gcagcacgca aaaaatttga tacgcgtaga aggttcagtc
     3481 aataaaattg ccgaacaatg tggttatgcc agtacatctt attttattta tgcgttccgc
     3541 aaacatttcg gcaacagtcc gaagagagtt tctaaggagt accgttgtca aagtcacacg
     3601 ggtatgaaca cgggcaacac gatgaatgct ttagctattt gattatttgc taacgagtag
     3661 tcaaccacac acgctacgta agaattaaat ggggcagcca ttccctgccc cgcgttgttt
     3721 ttaggcgata tattaattga aataaataag tg
//
LOCUS       DAPGWD010000253         3738 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPGWD010000253 DAPGWD010000000
VERSION     DAPGWD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.255"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(261..1451)
                     /locus_tag="RHX88_004328"
     CDS             complement(261..1451)
                     /locus_tag="RHX88_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2232930.1"
                     /translation="MPLTARQVETAKPKDKIYKIADGGGLYLQVNPNGSKYWRMKYHY
                     AGKEKKLSFGTYPTITLAEARKRRDEAKKIHADGKDPGEVKKAENLEKNLSEGRTFAA
                     IATEWYNAKVSGWSASYADYVDRAFKNNVFPYIGNRPISEIEPLELLAVLQRIESRGA
                     CELANKVRQRCGEVFRYAIVTGRARYNPASDLVIAMKSYKRTHYPFLLPPELPEFLYK
                     LENYTGSIVTREATKLLMLTGLRTVELRMGEWCEIDFTQKIWEVPPTRMKMRKEHIVP
                     LSDQAIQSLQILKELTGRYRYIFAGRNDVNRPISDASVNMVLKKIGYDKKATGHGFRH
                     TMSTVLHEQGFNSAWIEMQLAHTDKNSIRGTYNHARYLEGRREMMQWYADYLDALRKE
                     SPAI"
     gene            complement(1627..1701)
                     /locus_tag="RHX88_004329"
     tRNA            complement(1627..1701)
                     /locus_tag="RHX88_004329"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1666..1668),aa:Arg,seq:cct)
     gene            complement(1777..2709)
                     /locus_tag="RHX88_004330"
     CDS             complement(1777..2709)
                     /locus_tag="RHX88_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV2232931.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3003..>3738
                     /gene="mlaA"
                     /locus_tag="RHX88_004331"
     CDS             3003..>3738
                     /gene="mlaA"
                     /locus_tag="RHX88_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDV2232932.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDL"
BASE COUNT          901 a          861 c          881 g         1095 t
ORIGIN      
        1 atattaagat ataaatataa ctattgcgtt atttaaatta ttgtctatta cagctccagt
       61 taatagagcc tgaagtaatt tcttagtatt tcatcataag cgtttatcta tcgtctcttt
      121 ctaaattaac cggtcgtatt acaaggtgct gaatctgatt aaggaatgat acttagggga
      181 ttatggctga cttaataatt agataactga taaatgacta tcagggggat atcgttaagt
      241 cagcctttaa aatcaagcga ttatatagct ggtgactctt ttcgaagagc atcaaggtag
      301 tcagcgtacc attgcatcat ttctcgtctt ccttcaaggt aacgagcatg gttgtatgta
      361 ccacgaatgc tgtttttgtc cgtatgtgca agttgcattt caatccatgc actattgaat
      421 ccttgctcgt gtagaacagt actcattgta tgacgaaatc catgcccggt agcttttttg
      481 tcatagccaa tcttcttcaa aaccatattg acgcttgcat cgctgatagg cctgttaaca
      541 tcatttcttc cggcaaagat gtatctgtat cttccagtca gttctttgag gatttgcaga
      601 gactggattg cctgatctga cagagggacg atatgctcct tcctcatttt catccgggtc
      661 ggaggaactt cccatatctt ttgggtaaaa tcgatctcac accattcccc catacgtaat
      721 tctactgttc tcagccctgt gagcatcaac aattttgtag cttcacgggt tacaatactg
      781 ccggtgtagt tttcgagttt gtataaaaac tctggtagtt caggaggaag caggaaaggg
      841 taatgtgttc gcttatagct cttcattgct ataaccagat cagaagctgg attgtatcgt
      901 gcccgacctg taacaatggc ataccgaaac acttcaccac atcgttggcg aactttatta
      961 gctagctcgc aggctcctcg gctttcgata cgctgaagaa ctgcgagaag ttcaagaggt
     1021 tcgatttcac taatgggtct gtttccaatg tagggaaaca cattattttt gaatgctctg
     1081 tcgacataat cagcgtagct ggcagaccag cctgaaactt ttgcgttgta ccactcggtg
     1141 gcgattgcgg caaaagttct tccttcggaa aggtttttct caagattttc agctttcttg
     1201 acttcaccgg gatctttacc atcggcatgt attttcttgg cttcatcccg acgtttgcga
     1261 gcttcagcca atgtaatagt tggataggtg ccaaatgata gtttcttttc cttccccgca
     1321 tagtggtact tcattcgcca gtactttgag ccgttaggat tgacctgaag gtagagccca
     1381 ccaccatcgg caatcttata aattttatct ttgggtttgg ctgtttcaac ttgtcgagct
     1441 gtcagtggca tctgggggcc tcgcaataga ttgaccaaga tgtgccccta aataggcccc
     1501 caaatgtgac ttgatttacg ttcccctgag gagaattaag gcgactattt gagtaacata
     1561 agtaagggta tgtgctgatt ataaaggctt tttgtggggt atggtagatt tcaggagagg
     1621 tgtgcatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag gggctcgtta
     1681 tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga ctgcggggta
     1741 agtacgctgc cgctcgattg cttaaaccct cgccatttat gctgggtttt tatcattttt
     1801 cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct tgttgctcat
     1861 gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc cgcagatgtt
     1921 ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca gcgtgccgtt
     1981 aaacaccaga tagaggattt caacagaacc gacaacgatg tgggtggtgt cacccagtgc
     2041 aataagccag gtcatcaata taatcaccac aatctttgcc gcacccgcag caggaaacat
     2101 ccaaaccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca tctcgctggg
     2161 ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt cttcattgaa
     2221 gataggcata tattcaaatg cccatgcagc aatacctgtc ccgagaatat tacccagcag
     2281 cacgacgccc cataatcgca taagtaagcc gacgttgctc attgtcggtt tttgcatgac
     2341 gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca taatgacgat
     2401 aataaaacca aaggtataac cgagattctc cagtaagaag ctgcctggca ctccttccag
     2461 ttcgacatga aatatccctt ttgccagtag cgaagcgccc atcgacagac ccgccgcaat
     2521 ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat cctggcggat
     2581 atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt ctattttttt
     2641 gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat caattttgtc
     2701 gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc ggcttttttg
     2761 ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc ctttttctgt
     2821 ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca aatcagcaaa
     2881 tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta aacaggcgga
     2941 tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac agggagacat
     3001 ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg gggtgtgcga
     3061 gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac cgcaccatgt
     3121 acaacttcaa cttcaatgta ttagacccgt atattgttcg accggtcgct gtcgcctggc
     3181 gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc aaccttgaag
     3241 aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg atggtccact
     3301 ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat gttgcaggga
     3361 tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg cttggtcatt
     3421 atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc acgctgcgtg
     3481 atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg acctggccga
     3541 tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg ctggattccg
     3601 atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg tacttccagc
     3661 gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg aacgcacaag
     3721 cgattcagga tgatttaa
//
LOCUS       DAPGWD010000254         3697 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPGWD010000254 DAPGWD010000000
VERSION     DAPGWD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.256"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            138..1241
                     /gene="proB"
                     /locus_tag="RHX88_004332"
     CDS             138..1241
                     /gene="proB"
                     /locus_tag="RHX88_004332"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDV2232933.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1253..2506
                     /gene="proA"
                     /locus_tag="RHX88_004333"
     CDS             1253..2506
                     /gene="proA"
                     /locus_tag="RHX88_004333"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDV2232934.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2621..2696
                     /locus_tag="RHX88_004334"
     tRNA            2621..2696
                     /locus_tag="RHX88_004334"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2654..2656,aa:Thr,seq:cgt)
     gene            3307..3465
                     /locus_tag="RHX88_004335"
     CDS             3307..3465
                     /locus_tag="RHX88_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232935.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSD
                     LKWLREDF"
BASE COUNT          930 a          875 c          991 g          901 t
ORIGIN      
        1 taaacaactg ccgctaggct tgctgatccc gcgcaacaaa acgccatgct ttgctcgcag
       61 atggttggca accgacgaca gtcctgctaa aacgttcgtt tgatatcatt tttcctaaaa
      121 ttgaatggca gagaatcatg agtgacagcc agacgctggt ggtaaaactc ggcaccagtg
      181 tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat cgttgaactt gttcgccagt
      241 gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt gacgtcgggc gcgatcgccg
      301 ccggacgtga gcacctgggt tacccggaac tgccagcgac tatcgcctcg aaacaactgc
      361 tggcagcggt agggcagagt cgactgattc aactgtggga acagctgttt tcgatttatg
      421 gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat ggaagaccgt gaacgcttcc
      481 tgaacgcccg cgacaccctg cgtgcgttgc tcgataacaa tatcgttccg gtaatcaatg
      541 agaacgatgc tgtcgctacg gcagagatta aagtcggcga taacgataac ctttctgcgc
      601 tggcggcgat tctggcgggt gccgataaac tgttgctgct gaccgatcaa aaaggtttgt
      661 ataccgctga cccgcgcagc aatccgcagg cagaactgat taaagatgtt tacggcattg
      721 atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg cctcggaact ggcggcatga
      781 gtaccaaatt gcaggccgct gacgtggctt gccgtgcggg tatcgacacc attattgccg
      841 cgggcagcaa gccgggcgtt attggtgatg tgatggaagg catttccgtc ggtacgctgt
      901 tccatgccca ggcgactccg cttgaaaacc gtaaacgctg gattttcggt gcgccgccgg
      961 cgggtgaaat cacggtagat gaaggggcaa ctgccgccat tcttgaacgc ggcagctccc
     1021 tgttgccgaa aggcattaaa agcgtgactg gcaatttctc gcgtggtgaa gtcatccgca
     1081 tttgcaacct cgaaggtcgc gatatcgccc acggcgtcag tcgttacaac agcgatgcat
     1141 tacgccgtat tgccgggcac cactcgcaag aaattgatgc aatactggga tatgaatacg
     1201 gcccggttgc cgttcaccgt gatgacatga ttacccgtta aggagcaggc tgatgctgga
     1261 acaaatgggc attgccgcga agcaagcctc gtataaatta gcgcaactct ccagccgcga
     1321 aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa gcacaaagcg aaatcatcct
     1381 caacgctaac gcccaggatg ttgctgacgc gcgtgccaat ggccttggcg aagcgatgct
     1441 tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt gccgatgatg tgcgccaggt
     1501 gtgtaacctc gccgatccgg tggggcaggt aatcgatggc agcgtactgg acagcggcct
     1561 gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc gtgatttatg aagcgcgccc
     1621 gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc ggtaatgcgg tgatcctgcg
     1681 cggtggcaaa gaaacgtgtc gcactaacgc tgcaacggtg gcggtgattc aggacgccct
     1741 gaaatcctgc ggcttaccgg cgggtgccgt gcaggcgatt gataatcctg accgtgcgct
     1801 ggtcagtgaa atgctgcgta tggataaata catcgacatg ctgatcccgc gtggtggcgc
     1861 tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg gtgatcacag gtggtatagg
     1921 cgtatgccat atttacgttg atgaaagtgt agagatcgct gaagcattaa aagtgatcgt
     1981 caacgcgaaa actcagcgtc cgagcacatg taatacggtt gaaacgttgc tggtgaataa
     2041 aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa atggcggaaa gcggcgtgac
     2101 attacacgca gatgcagctg cactggcgca gttgcaggca ggccctgcga aggtggttgc
     2161 tgttaaagcc gaagagtatg acgatgagtt tctgtcatta gatttgaacg tcaaaatcgt
     2221 cagcgatctt gacgatgcca tcgcccatat tcgtgaacac ggcacacaac actccgatgc
     2281 gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt aacgaagtgg attcgtccgc
     2341 tgtttacgtt aacgcctcta cgcgttttac cgacggcggc cagtttggtc tgggtgcgga
     2401 agtggcggta agcacacaaa aactccacgc gcgtggccca atggggctgg aagcactgac
     2461 cacttacaag tggatcggca ttggtgatta caccattcgt gcgtaaataa aaccgggtga
     2521 tgcaaaagta gccatttgat tcacaaggcc attgacgcat cgcccggtta gttttaacct
     2581 tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg gccgatatag ctcagttggt
     2641 agagcagcgc attcgtaatg cgaaggtcgt aggttcgact cctattatcg gcaccatcaa
     2701 gaaaatcaat aacttagctt gacttctcta agaaattgta tcttctgtta tcacttacaa
     2761 acgcctatac gttgttatgg gctttggtag tgtaacacgt atgggcgagt agttcccagg
     2821 ctcaacctcg taacaagttc ctttgaagct ggaagacgtg ccaccgtctc taaaccttct
     2881 accaaaactc ttagcaagat gttccacacc tggcgcggtg tccaatgatg ctacacgtcg
     2941 tgagacgttg cagaggataa acaggctaaa tgatgggaag agtagtttta agtgcaatag
     3001 tggattaaag cccacctctt caaaacctaa cttgatcggg aaagaatcaa gctctagccc
     3061 cggtttcgtc caccggatta caacgggtgc ctgataagag aagaactgct aagacttcat
     3121 agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt tttggggtag
     3181 cccatcaaag gcgctttatg gctcctattg tcttgaaaat gtatctttac ttgtattgtg
     3241 ttttatgcgt agtctccctg tgtagtatgt aacttgttaa ttttcatatg aatacagagg
     3301 cacacgatga aaaacattgc agctatcaaa cgtaataacc gcaagattca cgctcgtaag
     3361 ttcctgtcta cgccagaagg aaaagcctgg ctagagcgta aacagagaga gaatgaagaa
     3421 agaaaactcc ttagtgattt gaaatggctt agagaggatt tctgaaaatc tccagaacgc
     3481 aacaggacgc attctaacgc tgttttcatt gctttgtata gggatgtata ggtgatgtgt
     3541 gtttaatgcg tctgtgagct tgttttgtgc cttattttgc attttcataa tgcagaagag
     3601 aacacggaaa accttaaaat taattattgg ggtatttaag gctattttta ggtgatttaa
     3661 gggtattaaa cttgatttaa ttggttaaaa tataaac
//
LOCUS       DAPGWD010000255         3696 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPGWD010000255 DAPGWD010000000
VERSION     DAPGWD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.257"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(92..466)
                     /locus_tag="RHX88_004336"
     CDS             complement(92..466)
                     /locus_tag="RHX88_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HDV2232936.1"
                     /translation="MLTFWVGFVAGCTPLHPSDCHKTTATGSCSSGRWDDQDEWGAQA
                     RGIRAAINAKLDEPHNWKGKKCRLHMEFSQDGTALKISTSNGDKAYCEAIKSAAHKAK
                     FPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(638..1066)
                     /gene="tolA"
                     /locus_tag="RHX88_004337"
     CDS             complement(638..1066)
                     /gene="tolA"
                     /locus_tag="RHX88_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV2232937.1"
                     /translation="MGFRSASILTLIISGIIIGCTDAVSTNYHDRTSYYSDKAIETQY
                     VSSSERTSDVSEDIRLYAHQIKSAIEKQFGDASKYSGKECTLRMHMAPNGLLLEVKRE
                     SGDLDLCREAMNAIKNADIPAPPSPEVYKVFQNGVLDFKP"
     gene            complement(2112..2654)
                     /locus_tag="RHX88_004338"
     CDS             complement(2112..2654)
                     /locus_tag="RHX88_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HDV2232938.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGRSGN
                     MFNQLLASKKLTKTAINEALRRMKKAGIEKAELEAFLREMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLICILRQRYEGRGMAKRKMAELLNDAHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAEKMA"
     gene            complement(2651..2941)
                     /locus_tag="RHX88_004339"
     CDS             complement(2651..2941)
                     /locus_tag="RHX88_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDV2232939.1"
                     /translation="MVNLRKAAKGQMCQIRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKKGHL"
     gene            complement(2941..3540)
                     /locus_tag="RHX88_004340"
     CDS             complement(2941..3540)
                     /locus_tag="RHX88_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDV2232940.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDEV
                     EFQQVYKSVLNVLWNWILFRKFSSQEEVENVAAQLLEFA"
BASE COUNT         1034 a          908 c          737 g         1017 t
ORIGIN      
        1 gtattagctc agacttgaca tcacaggttt cgtatataga acatcatcaa atctgtcagt
       61 ttgctatgaa tgagatatag taattgaaga gctaacctcg catgtcaaag ccagatttct
      121 gaaaatctct gtagacttcc ggattgttga aggccggaaa tttggcttta tgagctgcgg
      181 actttatcgc ttcgcaatag gctttatcac cgttactggt agatattttt aacgccgtgc
      241 catcctgaga gaattccata tgcaacctgc attttttccc tttccagtta tgcggctcat
      301 caagtttggc attaattgca gctctgattc cccgcgcttg cgccccccat tcatcctgat
      361 catcccagcg tcctgaactg caactacctg tagcagtagt tttgtggcaa tctgaagggt
      421 gtaaaggtgt gcatcccgca acaaaaccga cccaaaaagt caacataacg attttcttta
      481 atcccacttc ttgctcctca atccattaaa atctcagcaa tagtagttgt tacgtccgcc
      541 actggctcag agctgactat ccgctaaatt tagctcagtg ccgtagctgt gtcagaacaa
      601 acctaagccg aaaccgttta ttacaaaaca ataaatatca gggtttaaaa tccagcaccc
      661 cattttgaaa tactttatat acttccggcg aagggggggc aggtatatca gcattcttta
      721 tcgcattcat cgcttcacga cataaatcga ggtctccact ttctctttta acctccagta
      781 gaaggccatt cggggccata tgcattctca gtgtacactc ttttcctgaa tacttactcg
      841 catccccgaa ctgtttttcg atggcgctct tgatttgatg ggcatacaga cggatatcct
      901 cactaacatc agaagtacgt tcagatgaac tcacatactg tgtctctatt gctttatcgg
      961 agtaatatga tgtacggtca tgataatttg tcgatacagc atcagtgcac ccgataataa
     1021 tcccactaat aatcaacgta agaattgatg cgctacgaaa acccattttt cctcacatat
     1081 gtcatatagt aaaggattat atataccgtt gttttggacg ctcaaacagc gaatcagatc
     1141 aaataaaacg cacatttgtt aacatttaca caaagtctgc gtgggatatt ctgaaagaat
     1201 atccataatg tggagagaat ctattgaagt gcatggtgcc gggtgcctcc cggtgaacaa
     1261 aatgttcgtg atacctgtcg gcgacagaaa aggttaatgg tatcacccca ccgcacaggg
     1321 ggattcacca tgcaggagtt ttcttagcaa actcactgcg cgcccggcaa ctcccaacca
     1381 cataaaatgc ggagtttgtg gtatttatgc atataactcg caggaattat cttaaaaaac
     1441 tgatgtcgat ccggattaaa aagaagcagg tcatcatcag atgactggaa aaaaggaaaa
     1501 caaaaaatac tcatcataca gttttgattg cagggatgag cctgctatgc acaatatgca
     1561 gaatataagc aagataaaaa tatgcaggca tattatttcg gattttgtta ttaacacaac
     1621 ctttttaata atcatttggc atacaataaa ccagcccaaa aagaaccgcc taaacaggcg
     1681 gttggttaat acaaaggatg cttcgtcttt attatagtaa tctgaggcgt cgggtgtctt
     1741 gtatcagaca acatattgtc ccgctaaaca gcgaattaca aaccaccctg caatgatctc
     1801 tcatctcatt ttatatgagt tgacgacatc aggataacgc atcatcagcc cctgccaaga
     1861 aatatcaaaa ctcccgccag caatgtgtta tcacaatatt gtaaaaaaaa cacagcaccg
     1921 aaactataac tggtctctgt tataatttgg agcagaaaga ccagttgccc aactagcagc
     1981 attctcccct gctttcctga cgtaaaaaaa accgcattaa gcggtttttt tacgatgtcc
     2041 atgtctgcaa tccgcctcgc gatacagctt tgcgaagcat agcaaaattg aagcagttta
     2101 tacgtaagaa atcaagccat tttctcagca aatgattcac gcatgggaat atatagggca
     2161 tactcagcaa cagctaacca attagcaatc cgtttttcgc atgtgctaaa acaccactct
     2221 gggtgtgcat catttagcaa ttcagccatt ttgcgcttag ccatcccccg cccttcatag
     2281 cgttgccgga gaatgcaaat caatcctgga tgctctgcca gcacctcact tatgactcga
     2341 tcaatacata acgcctctgc atcagtacaa tgcgccagcc agctcttttg cttgccgttg
     2401 atcatctctc gcaaaaacgc ttccagctca gctttctcta ttcccgcttt tttcattctg
     2461 cgcagggctt cattaatggc tgttttcgtc agttttttgg atgccaacaa ctgattgaac
     2521 atattccctg acctgccacc gccaatatac gaccagcgcc cccacatacg tagttttccc
     2581 tgaatccaga cactttccag cgtggtgagg cgaaggtgtt ctccgctttt tcctgtattc
     2641 gttgggtaaa tcataaatga cctttctttc tccagatttc ttgtgtgcga aaaacccctt
     2701 cagcatgcat caggcgcaat tcttctttgg tgtaatcgct ggtttttacc cgcccgtcga
     2761 ttagatcgtg gcatgagcta caggctatcg ccgcctgcat atcgtgtggt tttgtcgctg
     2821 ttccgcacgt ccccgccagc ctgtaatgcg ccagcacaga ggtttcggga ttgtgattgc
     2881 agtagccagg gattctgatc tggcacatct ggcctttagc cgctttacgt aaattcacca
     2941 ttacgcaaac tccagtagtt gtgcggccac attttcaact tcctcctgag aggagaattt
     3001 acggaacaga atccagttcc acagcacatt cagtacagat ttataaacct gctgaaactc
     3061 gacttcgtcc atattcgcaa aagcgatgga ttttgcccga cgcccacggc taccgtccgg
     3121 ataaatatgc tcggtgtaaa atccggcctg aatggttacc cactcgcgga aagcctcaaa
     3181 cgactttagc aacgccgtat cccgggttct acgagtcgca acggtgttaa ggtattgctc
     3241 tgcggcatca ctcagggctg gcgtgtgttc ccgaccaact gattcgcaca ggtaatcaac
     3301 gaaaccagac agcagttctc gttcgcgagg cgtgatcgcc ccaccgaccg gagtccagta
     3361 atcgaatccc agttgcagga gtttgaaaaa acgcttgtgg aatgcgtagt tacgcacacg
     3421 cttaaagtct gcgtgtatcc actcacctat tttgatttga tgcagaaaat cgcaactctc
     3481 cggcgtcgcc gggagaagta aaccagaaga agtttgtttg accagttgta tatgcgccat
     3541 ttctcaatct ctcgatggcg cagtgcagca gatgccagtt gttcaggctg acgtataaag
     3601 tataaataaa ctggttccag tgtaaagccc ccaccttaat ggaataaaga ccaaacaaca
     3661 gattgctggg atacaaacaa cgcttattat taaaag
//
LOCUS       DAPGWD010000256         3535 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPGWD010000256 DAPGWD010000000
VERSION     DAPGWD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.258"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..634
                     /gene="fimC"
                     /locus_tag="RHX88_004341"
     CDS             <1..634
                     /gene="fimC"
                     /locus_tag="RHX88_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV2232941.1"
                     /translation="THAAGGIALGATRIIYPADAQQTAVWIRNSHTNERFLVNSWIEN
                     SSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMNVKTIPSVDKNAL
                     NGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINVHNPSPFYVTLVNL
                     QVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVREVNLN"
     gene            665..3274
                     /locus_tag="RHX88_004342"
     CDS             665..3274
                     /locus_tag="RHX88_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV2232942.1"
                     /translation="MKIPTTTDIPQRYSWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPARVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQITL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDELIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            3287..>3535
                     /locus_tag="RHX88_004343"
     CDS             3287..>3535
                     /locus_tag="RHX88_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2232943.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYV"
BASE COUNT          927 a          785 c          866 g          957 t
ORIGIN      
        1 cacccatgct gccggaggga tcgcattagg tgccacgcgt attatttatc ccgctgatgc
       61 tcaacagact gcggtatgga ttagaaatag ccataccaat gagcgctttc tggtcaattc
      121 gtggattgaa aacagcagcg gtgtaaaaga aaagtcattc atcattacac cgccactgtt
      181 tgttagtgaa cccaaaagcg aaaatacctt gcgtattatt tacaccggtc caccgctggc
      241 ggcagatcgt gagtctctgt tctggatgaa tgttaagacg atcccttcgg tagataaaaa
      301 tgcattgaac ggcaggaatg ttttgcaact ggcgatttta tcgcgcatga aattatttct
      361 ccgtccaatt caattacaag aattacccgc agaagcgccg gacacactca agttttcgcg
      421 atccggtaac tatatcaatg ttcataatcc ctcacctttt tatgtcaccc tggttaactt
      481 acaagtgggc agccaaaagt tggggaatgc tatggctgca cccagagtta attcacaaat
      541 tcccttaccc tcaggagtgc agggaaagct gaaatttcag accgttaatg attatggttc
      601 agtaaccccg gtcagagaag tgaacttaaa ctaaccgaat catctgacaa tatcagagct
      661 aattatgaaa atacccacta ctacggatat tccgcagagg tatagctggt gtctggccgg
      721 aatttgttat tcatctcttg ccattttacc ctccttttta agctatgcgg aaagttattt
      781 caacccggca tttttattag agaatggcac atccgttgct gatttatcgc gctttgagag
      841 aggtaatcat caacctgcgc gcgtgtatcg ggtggatctc tggcgtaatg atgagttcat
      901 tggttcacag gatatcgtat ttgaatcgac aacagaaaat acaggtgata aatcaggtgg
      961 gttaatgccc tgttttaacc aggtactcct tgaacgaatt ggccttaata gcagtgcatt
     1021 tcccgagtta gcccagcagc aaaacaataa atgcatcaat ttactgaaag ctgtacctga
     1081 tgccacaatt aactttgatt ttgcagcgat gcgcctgaac atcactattc ctcagataac
     1141 gttgttgagt agcgctcacg gttacattcc gcctgaagag tgggatgaag gtattcctgc
     1201 tttactcctg aattataatt tcaccggtaa cagaggtaat ggtaacgata gctatttttt
     1261 tagtgagctc agcgggatta atattggccc gtggcgttta cgcaacaatg gttcctggaa
     1321 ctattttcgc ggaaatggat atcattcaga acagtggaat aatattggca cctgggtaca
     1381 gcgcgccatt attccgctga aaagtgaact ggtaatggga gacggcaata caggaagtga
     1441 tattttcgat ggtgttggat ttcgtggtgt acggctttat tcttccgata atatgtatcc
     1501 tgatagccag caagggtttg ccccaacggt acgtgggatt gcccgtacgg cggcccagct
     1561 aacgattcgg caaaatggtt ttattatcta tcaaagctat gtttcccccg gcgcttttga
     1621 aattacagat ttgcacccga catcttcaaa tggcgatctg gatgtcacca tcgacgagcg
     1681 cgatggcaat cagcagaatt acacaattcc gtattcaaca gtgccgattt tacaacgcga
     1741 agggcgtttc aaatttgacc tgacggcggg cgattttcgt agcggtaata gtcagcaatc
     1801 atcacctttc ttttttcagg gcacggcact cggcggttta ccacaggaat ttactgccta
     1861 cggcgggacg caattatctg ccaattacac cgccttttta ttaggactgg ggcgcaatct
     1921 cggaaactgg ggggcagtgt cgctggatgt gacccatgcg cgcagtcagt tagccgacga
     1981 cagtcgtcat gagggggatt ccattcgctt cctctatgcg aaatcgatga acaccttcgg
     2041 caccaatttt cagttaatgg gttaccgcta ttcgacacaa ggtttttata cccttgatga
     2101 tgttgcgtat cgtcgaatgg aggggtacga atatgattac gattacgacg gtgagcatcg
     2161 ggatgaactg ataatcgtga attaccacaa tttacgcttt agccgtaaag accgtttgca
     2221 gttaaatatt tcacaatcac ttaatgactt tggctcgctt tatatctctg gtacccatca
     2281 aaaatactgg aatacttcgg attcagatac ctggtatcag gtggggtata ccagcagctg
     2341 ggttggcatc agttattcgc tctcattttc gtggaatgaa tctgtaggga tccccgataa
     2401 cgaacgtatt gtcggactta atgtttcagt gcctttcaat gttctgacca aacgtcgcta
     2461 cacccgggaa aatgcgctcg accgcgctta tgcctccttt aacgccaacc gtaacagcaa
     2521 cgggcaaaat agctggctgg caggtgtagg tgggacctta ctggaaggcc acaacctgag
     2581 ttatcacgta agccagggtg atacctcgaa taatgggtat acgggcagcg ccacggcaaa
     2641 ctggcaggcc gcttacggta cgctgggggt cgggtataac tacgaccgcg atcaacatga
     2701 cgttaactgg cagctgtctg gcggtgtggt cgggcacgaa aatggcataa cgctgagcca
     2761 gcctttaggg gataccaatg ttttgattaa agcgcctggc gcaggcggtg tacgcattga
     2821 aaatcaaact ggcattttaa ccgactggcg cggctatgcg gtgatgccgt atgccacggt
     2881 ttatcggtat aaccgtatcg cgcttgatac caatacgatg gggaattcca tcgatgttga
     2941 aaaaaatatc agcagcgttg tgccgacgca aggcgcgttg gttcgtgcca attttgatac
     3001 ccgcataggc gtgcgggcgc tcattaccgt tacccagggc ggaaaaccgg tgccgtttgg
     3061 atcactggta cgggaaaaca gtaccggaat aaccagtatg gtgggtgatg acgggcaagt
     3121 ttatttaagt ggtgcgccat tgtctggtga attactggtt cagtggggag acggcgcgaa
     3181 ctcacgctgc attgcgcact atgtattgcc gaagcaaagc ttacagcaag ccgtcactgt
     3241 tatttcggca gtttgcacac atcctggctc ataaaggaaa ttatcaatga agataatctg
     3301 cagattatta ttggcgatgg catgtttgtg tctggcaaac atatcctggg ctactgtttg
     3361 tgcaaatagt actggcgtag cagaagatga acactatgat ctctcaaata tctttaatag
     3421 caccaataac cagccagggc agattgttgt tttaccggaa aaatccggct gggtaggtgt
     3481 gtcagcaatt tgtccacccg gcacgctggt gaattataca taccgtagtt atgtc
//
LOCUS       DAPGWD010000257         3518 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPGWD010000257 DAPGWD010000000
VERSION     DAPGWD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.259"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(89..946)
                     /gene="sitD"
                     /locus_tag="RHX88_004344"
     CDS             complement(89..946)
                     /gene="sitD"
                     /locus_tag="RHX88_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDV2232944.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFIAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDIHLDHILFGDMLGVTIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVISFNARTKGNA
                     HRQDLLSPN"
     gene            complement(943..1800)
                     /gene="sitC"
                     /locus_tag="RHX88_004345"
     CDS             complement(943..1800)
                     /gene="sitC"
                     /locus_tag="RHX88_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDV2232945.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1797..2624)
                     /gene="sitB"
                     /locus_tag="RHX88_004346"
     CDS             complement(1797..2624)
                     /gene="sitB"
                     /locus_tag="RHX88_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDV2232946.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNASEESIITDDEHPFVAHRPSAVQREAR"
     gene            complement(2624..>3518)
                     /gene="sitA"
                     /locus_tag="RHX88_004347"
     CDS             complement(2624..>3518)
                     /gene="sitA"
                     /locus_tag="RHX88_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HDV2232947.1"
                     /translation="TMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAA
                     EVSSITKPGAEIHEYQPTPGDIKRAQEAQLILANGMNLELWFQRFYQHLNGVPEVIVS
                     SGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYK
                     AKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQ
                     VRKVVDMVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYI
                     DLLKVTTSTLVQGIKAGKREK"
BASE COUNT          935 a          925 c          858 g          800 t
ORIGIN      
        1 tgggaggatt tgcacctcct cccaacgtcc gcccatgact ttctggactg cagtgtacta
       61 gcggacaatg ctgccctcgg atgttgtgct aattggggga taacagatcc tgtctatgag
      121 cgtttccctt tgtgcgtgcg ttgaaactga taacggtaaa cgtcatgatg aacacaaccg
      181 cgaataagac gacaatcgtc ggagctggtg cgctgtcgat aaaaaaggac agccaaacac
      241 cagtcattga gactattacc gatatgccag tcgccagtag gagtgcaata tgaaagcgtt
      301 gtgtcaggag taccgcaatg gcgccaggcg caatgagcag agaaatggag agaatgatac
      361 ctactgcttt cagcgtcgcc acaatggtca gggaaaccat gcacagcagc ccataatgca
      421 gccatcgcgt atgcaaaccg cttacctgcg cctgttgata atcaaagctg aacagcaaaa
      481 aatcccgcca ttttacgcta ataacaagtg taaccagccc ggcaataatc atcgtctgga
      541 ttatatcgcc gatggttacc ccgagcatgt cgccgaaaag aatatggtca agatgtatgt
      601 ctggtttgac tgcaatatac aagatcagac ctgcggcaaa catgccagaa aaaacaatcc
      661 ccatcactgt atcttgcttg attcggctgt tgtctttcag gtatcccgtc gccaccgcgc
      721 agaatactcc ggcgataaat gcccctgtag ccagaggtaa ccccagaatc caggcaagca
      781 cgatgccagg aaaaaccgca tgactcatcg cgtcacccat cagtgcccaa cctttcagga
      841 ccagaaagac cgacagcagc gcacagggta tggaaactac cagtgaaatc agcagtgcgt
      901 gactcataaa ggtgaactgc agtggctcca gtaataatgc catcatgagc gatcctccag
      961 cgctttatgc gcacgccgac gatttgccaa aagcccatgg gtgggcgcaa atacaaatgc
     1021 taaaagaaac agcagtgtct gcgccaccac gatgattcca cctgtcgcgc cgtccagaaa
     1081 gtaactgacc cacgcgccga ggaaactggt tacactgcca atagttacag caattatgag
     1141 caggcgggga aaacgatcgg ttaacaacca tgctgttgca ccgggagtca ccaccagaca
     1201 gatcaccaga aaggcaccaa ccgtttgcag cgccgcaacg gtcgaaaccg acagcagggt
     1261 gaagaagagg atttttaatc tgcccggatg caatccaata gcacgggcgt gattctcgtc
     1321 gaaaaaggtc accataagat ctttccattt gaaaaaaagc acgatgatcg agagaatgcc
     1381 aatgatagtg agttgcagga tatccgccgg atcgattgcc agaatattgc ctaggacgat
     1441 ggtctgaatg ttcacggatg tcgggttcaa tgacaccata aacagcccca ggccaaaaaa
     1501 ggaggagaag atcaggccga tgatggcatc ttcttttaag cgagtgcgtt ggttaagaaa
     1561 cagcatgctg cccgctgcca gtccgccgga aaagaaggct cccagagaaa acggaagccc
     1621 cagcatatat gcccccgcaa cacccgggac aatagagtgc gacagcgcat cgccaatcag
     1681 cgaccagcct tttagcatca gatagcatga caggaaagcg cagagaccgc cgaccatcgc
     1741 cgagacccac atcgcattga gcatatattc atagctgaag ggttccagta gcacgttcat
     1801 cttgcttccc tctgcaccgc tgacggtcga tgtgccacaa aagggtgttc atcgtcagta
     1861 ataatgcttt cttccgaggc gttaagtgtg acatggcgca gtacgccgct aaaagccagc
     1921 tccaggttct cggctgtaaa agtggtgtct gtcggcccgc ttgccaggac ggtgcctttg
     1981 accatgaccg tataatcgca aaatgtcgtg actgagccga gattgtgcgt tgagacaagc
     2041 atcgtcttgc cttccgcgcg taactccctc aacaggctga tgattttcgc ttcggttttc
     2101 acatcaacgc cggtaaaagg ttcatccagc aggatcacat caccctgctg tgcaatcgct
     2161 ctcgccagaa agacgcgttt cttttgtcca ccggaaagct cgccgatttg tcgatggcga
     2221 aaatccacca tatcgacacg ttccagcgca tcggtgacaa tctgacgatc cctctttttg
     2281 gctatacgca acatacccat gtgcccatag cggcccatca ttaccacatc ttccactagt
     2341 acaggaaatg accagtcaac ctcttctgac tgagggacgt aggcaaccag gtttttctgt
     2401 agcgcctgtc gcgtgggaat acccagaaca gatatctttc cgctggtcag acgcacaaat
     2461 cccataatcg ctttaaacaa cgtcgatttt ccggaaccgt ttaccccaac cagagcggca
     2521 atagatccac ttggtactgt gaaggatgca tcgcgcagtg ctgtgtgccc gttacgccag
     2581 gtgacagtaa catcattaac gacaatgccc gcagattgca tcattatttc tcccttttcc
     2641 cggctttaat tccctgtacc agcgtactgg tagtaacctt cagaaggtcg atatacgtag
     2701 gtaccgggcc gttttctgtg ctcagggaat cgacatagag tacaccgccg tagtgcgcgc
     2761 cggtttcacg cgcaacctga cgcgctggtt tatcggaaat cgtactctcg ctaaagactg
     2821 ccgggatatg atttttcttc accatatcaa caaccttacg tacctgctgc ggcgtgcctt
     2881 gttgatcggc attaatcggc cacagataaa gctcttttag ccccaaatcg cgtgcgagat
     2941 aagaaaaagc cccttcactg gtgaccatcc atcgctgatt ctcagggagt tcagcaatct
     3001 gcttacgcag gggggcaagg gtttgggtaa tcttggcttt ataagtatcg gcattacgtt
     3061 ggtaggtttg tgcatttgcc ggatcgtatt ttatcaacgc atcacgaata ttatcgacgt
     3121 aaatcagagc attatctggc gacatccagg catgggggtt aggtttgccc tcataggggc
     3181 cttcagtgat ccctactggc gtcacacccg aagagacaat tacttctgga accccattaa
     3241 gatgctggta aaagcgttgg aaccacaatt ccagattcat accattggcg agaatcagtt
     3301 gtgcctcctg cgcacgttta atatcgccag gggtaggctg atactcatga atttctgcac
     3361 cgggcttggt tatggatgag acttctgcag catctccagc cacgtttttg gccatatctg
     3421 cgatgatggt aaatgttgta atgaccttga atttttcagt tgcactcgcc tgaaatgcga
     3481 tcgagcaggt gagggcgagg caccccaaga gcatggtt
//
LOCUS       DAPGWD010000258         3330 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPGWD010000258 DAPGWD010000000
VERSION     DAPGWD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.260"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            315..1631
                     /gene="shiA"
                     /locus_tag="RHX88_004348"
     CDS             315..1631
                     /gene="shiA"
                     /locus_tag="RHX88_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDV2232948.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSSFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQHA"
     gene            1733..3187
                     /gene="amn"
                     /locus_tag="RHX88_004349"
     CDS             1733..3187
                     /gene="amn"
                     /locus_tag="RHX88_004349"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDV2232949.1"
                     /translation="MNNKGSGLTPAQALEKLDALYEQSVVALRNAIGNYIKSGELPDE
                     NARKEGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITTDGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          778 a          805 c          799 g          948 t
ORIGIN      
        1 taaaaacata cctaacctcg cctccctact ggttaaaatg caatgcagtc tatcagaatc
       61 atcagggtgc aattttgtgc atatgcggac ttttatgttt catatctcaa aactgtaggt
      121 gctctgctca atccttatgc aactcctgcg atttcatctt ccatcgaatt tttcatgttg
      181 ccgttaatca gccataaaat catttgcaga tgcgctctgt cgaggtagtc tcataaggtt
      241 cgtttataga tcgacggcaa tgtgagttac cttttccata ctaattataa aaagacagta
      301 caaacaggat cattatggac tccacgctca tctccactcg tcccgatgaa gggacgcttt
      361 cgttaagtcg cgcccgacga gctgcgttag gcagtttcgc tggtgccgtc gtcgactggt
      421 atgatttttt actctatggc attaccgccg cactggtgtt taatcgcgag tttttcccgc
      481 aagtaagccc ggctatggga acgctcgccg catttgccac ctttggtgtc ggattcctgt
      541 tccgtccgct cggcggtgtc attttcggtc actttggcga ccgactggga cgtaagcgca
      601 tgttaatgct gaccgtctgg atgatgggca tcgcgacagc cttgattggc attcttcctt
      661 ccttctcgac cataggttgg tgggccccca ttttactggt gacactgcgt gccattcagg
      721 gatttgccgt cggcggcgaa tggggaggcg cggcgttact ttccgttgaa agtgcaccga
      781 aaaataaaaa agccttttac agtagcggtg tacaagttgg ctacggagta ggtttactgc
      841 tttcgaccgg actggtttca ttgatcagta tgatgacgac tgacgaacag tttttaagct
      901 ggggctggcg cattcctttc ctgtttagca tcgtactggt actgggggca ttgtgggtgc
      961 gtaatggcat ggaggagtcc gcggaattcg aacaacagca acataatcaa gcggccgcga
     1021 aaaaacgcat tccggttatc gaagcactat tacggcatcc cggtgctttc ctgaagatta
     1081 ttgcgctacg actgtgcgag ttgctgacga tgtacatcgt taccgccttc gcacttaatt
     1141 attcaaccca gaatatgggg ttaccgcgcg aacttttcct caatattggt ttgctggtag
     1201 gtggactaag ctgtctgaca attccctgtt ttgcctggct tgccgatcgt tttggtcgcc
     1261 gcagagttta tatcacaggt gcgttaatcg gaacgttgag ctcatttcct ttctttatgg
     1321 cgcttgaagc acaatctatt ttctggatag ttttcttctc cataatgctg gcaaacattg
     1381 cgcatgacat ggtggtgtgc gtgcaacaac cgatgtttac cgaaatgttt ggtgccagtt
     1441 atcgctatag tggcgctgga gtcggatatc aggttgccag tgtggtcggc ggtggattta
     1501 caccttttat tgctgctgca ctcatcactt attttgctgg aaactggcat agcgtcgcca
     1561 tttatttgct ggctggatgc ctcatttctg caataaccgc tttgttgatg aaagacaatc
     1621 aacacgcttg atagtcaggc gaagatcatc cgatcttcgc cttacacttt tgtttcacat
     1681 tcctgtgaca tactatcgga agtgcggtaa ttgtatggaa caggagacac acatgaataa
     1741 taagggctcc ggtctgaccc cagctcaggc actggaaaaa ctcgacgcgc tgtatgagca
     1801 atctgtagtc gctttacgca atgccattgg caactatatt aaaagtggcg aattacctga
     1861 tgaaaatgcc cgtaaagaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg
     1921 tagcacaaca aatcccccca aaacgcgcgc atttggtcgt tttactcacg caggcagcta
     1981 caccaccacg attactcgcc ctactctctt tcgttcgtac ctgaatgagc aactaacgtt
     2041 gctttatcag gattatggtg cgcatatttc tgtgcaaccc tcgcaacatg aaatccctta
     2101 tccttatgtc atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctggattaac
     2161 tcgctacttc ccgacaacag aactggcgca aatcggcgat gaaactgcag acggcattta
     2221 tcatccaacg gaattctccc cgctatctca ttttgatgcg cgccgggttg atttttccct
     2281 cgcacggttg cgccattata ccggtacgcc agtagaacat tttcagccgt tcgtcttgtt
     2341 taccaactac acacgttatg tggatgagtt cgttcgttgg ggatgcagcc agatcctcga
     2401 tcctgatagt ccctacattg ccctttcttg tgctggcggg aactggatca ccgccgaaac
     2461 cgaagcgcca gaagaagcca tttccgacct tgcatggaaa aaacaccaga tgccagcatg
     2521 gcatttaatt accaccgatg gtcagggtat tactctggtg aatattggcg tgggaccgtc
     2581 aaatgctaaa accatctgcg atcatctggc ggtgctgcgc ccggatgtct ggttgatgat
     2641 tggtcactgt ggtggcttac gtgaaagcca ggccattgga gattatgtac ttgcacacgc
     2701 ttatttacgc gatgaccacg ttcttgatgc ggttctgccg cccgatattc ctattccgag
     2761 cattgctgaa gtgcaacgtg cgctttatga cgctaccaag ttagtcagcg gcaggcctgg
     2821 tgaagaagtc aaacagcggt tacgtactgg cactgtggta accacagatg acaggaactg
     2881 ggagttacgt tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga
     2941 tatggaaagt gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact
     3001 actgtgtgtt tcagataaac cgttacatgg tgagattaag cttcccggcc aggctaaccg
     3061 tttttatgaa ggcgctattt ccgaacacct gcaaattggc attcgggcga tcgatttgct
     3121 gcgcgcagaa ggcgaccgac tgcattcacg taaattacga acctttaatg aaccgccgtt
     3181 ccggtaataa gttctgttcc tttttatcag gaatagatgt gaaggattta gaaagtcatc
     3241 cagcaaatag cctgcaataa agcaggctgt ttgcttcctg gggcagatgc gagttttatt
     3301 atcgtcttaa tgatttccac atattaaaag
//
LOCUS       DAPGWD010000259         3219 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPGWD010000259 DAPGWD010000000
VERSION     DAPGWD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.261"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            844..1821
                     /locus_tag="RHX88_004350"
     CDS             844..1821
                     /locus_tag="RHX88_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV2232950.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(2100..2840)
                     /locus_tag="RHX88_004351"
     CDS             complement(2100..2840)
                     /locus_tag="RHX88_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2232951.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(2961..3191)
                     /locus_tag="RHX88_004352"
                     /pseudo
     CDS             complement(2961..3191)
                     /locus_tag="RHX88_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
BASE COUNT          788 a          810 c          826 g          795 t
ORIGIN      
        1 caggcgtgtt tttctgttgc gtcagctgtt gttcagctct ggttaatgca ttacgggccg
       61 tcttaagcga ttccgtcact gtcaacgcca cgatctttga ccgtcttata tcactggcgc
      121 tgacagagtt atccggcatg gtgtcaatca gttcacaatt tttccatcgg agcagcctac
      181 caccggtgat aacctgactt tgcctgtagt ttgtatacac aacccctggc attgaaacaa
      241 acatatgaag tgtacttcgg atatgactgt acgctcagga agtacggcag attccgtcaa
      301 aaattacctg taaagacaga cttcagaaaa aacggtgctc gccgcagacc ggtgaccggg
      361 cgaagcccgc gaaccggcga ggaagcgcaa tatatctgta gttacagtga gcaggtacgc
      421 cctgaagcct tcataatcgg tagtgtatgc tgtttttccg gaatgattta tccacatatc
      481 cagaggcccg atccagtaaa tagatccatg aatgatcaat aaaggatcca ttaaagatcc
      541 ccatatcgct acaaaccttg tcactcatgg gccgggacca cgttcacata agctgtggca
      601 tgttatggat aaactgtaac aggttcatga taagctgtat tcagtaatgc atatactgaa
      661 gtaagttaat gacataaact atggtcagta cgccagactc agttgttaaa tacagactgc
      721 aggtttttct tcagtcagtt gacggggttc tgacacacga ttttctgttt attcttttac
      781 tgtccacagg caggaggctt tctggaaaac gaaaattcag acatcaaaaa actgttcggc
      841 gaggtggata agtcgtccgg tgagctggtg acactgacac caaacaataa caacaccgta
      901 caacctgtgg cgctgatgcg tctgggcgtt tttgtaccga cccttaaatc actgaagaac
      961 agtaaaaaaa atacactgtc acgtactgat gccacggaag agctgactcg tctttccctg
     1021 gcccgtgctg agggattcga taaggttgag atcaccggcc cccgcctgga tatggataac
     1081 gatttcaaga cctgggtggg gatcattcat tcctttgccc gccataacgt gattggtgac
     1141 aaagttgaac tgccttttgt tgagtttgca aaactgtgtg gtataccttc aagccagtca
     1201 tcccgcaggc tgcgtgagcg catcagccct tccctgaagc gcattgccgg taccgtgatc
     1261 tcgttttccc gtaccgatga gaagcacacc cgggaataca tcacccatct ggtacagtca
     1321 gcctactacg atactgagcg ggatattgtt cagttacagg ctgatccccg cctgtttgaa
     1381 ctgtaccagt ttgacagaaa agtccttctc cagcttaagg cgattaatgc cctgaagcga
     1441 cgggagtccg cccaggcact ctacaccttt atagagagcc tgccccggga tccggcaccg
     1501 atatcgctgg cgcggctgcg tgcacgcctc aatctgaagt ctcctgtatt ttcccagaac
     1561 cagacggtca gacgggcaat ggagcagttg cgcgagattg gatatcttga ttacacggag
     1621 atccagcggg ggcggacaaa attcttctgt attcactacc ggcgtccccg gttaaaagcg
     1681 ccgaatgatg agagtaagga aaatccgttg ccaccttcac ctgcggaaaa agtcagtccg
     1741 gagatggcgg agaaacttgc cctgcttgaa aaactgggca tcacactgga tgacctggaa
     1801 aaactcttca aatcccgctg aacataaact gtagtcagtg aagagtgctc ctttactgac
     1861 tacagcttat attatcaggt gcagtgagtg gtctgctcac tgcagtttat attcagtttc
     1921 ctgcagtgct gcctgtggct gagctgccat ctgcctgtcc cttacgtgag tcaccccgta
     1981 acctgatgct gaggcattgc tcccttcata aaacatggtt tactcactac agcttatcta
     2041 cctgctccag cttatgttat gtctgttctg ctgaccactg cttatataag gaagtgccgt
     2101 tacttcaggg gaggcagtgt acgcaggatc tccgcagcat cccgaccgtc acctgtgaaa
     2161 ggcactgcca gcgtggcagc catatccagt gcaaacactc tggtataaac ctccatcgaa
     2221 cgtggatccc tgtgaccagc cagtgcctgg atgactttcc ggggctggcg gtgatagagc
     2281 atgtgcatga tatagctgtg ccggaaggtg tgtggtgtga ccggaatcga aaagtgtact
     2341 ccgtcagctt cggcccgtct gacagcctgc ttcagccagt tgcgcatggt ttcgtcggtc
     2401 acggcccata atggttcacg acgacggggc cgggtggtga tcatccagct ttccatctgc
     2461 ctgacatagc ttatatctgt cagcggaacc aggcgcactt catcttttgg cgggcgtccg
     2521 cgtcgcgcac gcactttttc ggacaggatc cgcacaaacg gtcttacacc atccaggtca
     2581 aatgattccg gcgtcagcat ccgggcttcg ccaatacgca ttccggtatt ccagagggtg
     2641 gcgaacagca tatggtgacg ctgatccggc atatagaaga gaagggcact cacttccggg
     2701 gccagcaggt aggccggtgt ggcccccgtg gaagtggcca ttcttctcag ggagagcgct
     2761 gttgcaaaat ccacccccgg cgcaatgggt aacagggaga cgcgttctgg tgaattctgc
     2821 aggggaatga cattgttcat gagttatttt tcacgtagca cacgcactgt ttatggatac
     2881 aggtattctg acctgaacgc ccggatcctt ccacgacaat aaatcgcagc gcgcggatcc
     2941 ttttgtttgc ttatcacgca ttattgcggg taaaaccaaa tacgtaaagg tacgttttgc
     3001 agcagggatc ccgtattaag ccagtcgtgg cgcgggttga gggctggttt gccgtttttg
     3061 ggagaattgg ggtaatgcca ggatgaatgg gatcggagaa aacagagaaa gggaacagaa
     3121 agggagaagg tgcaccacgt gtattctggc ccgggacgtc accgtccccc tgacagtttg
     3181 actttacaca ggaaggttgc agactcagca taatgactg
//
LOCUS       DAPGWD010000260         3202 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPGWD010000260 DAPGWD010000000
VERSION     DAPGWD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.262"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            162..1844
                     /locus_tag="RHX88_004353"
     CDS             162..1844
                     /locus_tag="RHX88_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDV2232952.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1896..3053
                     /locus_tag="RHX88_004354"
     CDS             1896..3053
                     /locus_tag="RHX88_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV2232953.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNLVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3019..3139)
                     /locus_tag="RHX88_004355"
                     /pseudo
     CDS             complement(3019..3139)
                     /locus_tag="RHX88_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          971 a          686 c          645 g          900 t
ORIGIN      
        1 tacttagtag aacagaaagt aatgtcaacg ctttatggac tgttatttcc ctttttttag
       61 ctaaatctgc tatctcttta tgtgactaac ttcacttaca tccacttatt tctcttcgta
      121 aaattacttt ggaattaagt acaataagaa gaggaacatt tatgaagtct gcattaaaga
      181 aaagtgtcgt aagtacctcg atatctttga tactggcatc tggtatggct gcatttgctg
      241 ctcatgcggc agatgatgta aagctgaaag caaccaaaac aaacgttgct ttctcagact
      301 ttacgccgac agaatacagt accaaaggaa agccaaatat tatcgtactg accatggatg
      361 atcttggtta tggacaactt ccttttgata agggatcttt tgacccaaaa acaatggaaa
      421 atcgtgaagt tgtcgatacc tacaaaatag ggatagataa agccattgaa gctgcacaaa
      481 aatcaacgcc gacgctcctt tcattaatgg atgaaggcgt acgttttact aacggctatg
      541 tggcacacgg tgtttccggc ccctcccgcg ccgcaataat gaccggtcga gctcccgccc
      601 gctttggtgt ctattccaat accgatgctc aggatggtat tccgctaaca gaaactttct
      661 tgcctgaatt attccagaat catggttatt acactgcagc agtaggtaaa tggcacttgt
      721 caaaaatcag taatgtgccg gtaccggaag ataaacaaac acgtgactat catgacaact
      781 tcaccacatt ttctgcggaa gaatggcaac cgcaaaaccg tggctttgat tactttatgg
      841 gattccacgc tgcaggaacg gcatattaca actccccttc actgttcaaa aatcgtgaac
      901 gtgtccccgc aaaaggttat atcagcgatc agttaaccga tgaggcaatt ggcgttgttg
      961 atcgtgccaa aacacttgac cagcctttta tgctttacct ggcttataat gctccgcacc
     1021 tgccaaatga taatcctgca ccggatcaat atcagaagca atttaatacc ggtagtcaaa
     1081 cggcagataa ctactacgct tccgtttatt ctgttgatca gggtgtaaaa cgcattctcg
     1141 aacaactgaa gaaaaacgga cagtatgaca atacaattat tctctttacc tccgataatg
     1201 gtgcggttat cgatggtcct ctgccgctga acggggcgca aaaaggctat aagagtcaga
     1261 cctatcctgg cggtactcac accccaatgt ttatgtggtg gaaaggaaaa cttcaacccg
     1321 gtaattatga caagctgatt tccgcaatgg atttctaccc gacagctctt gatgcagccg
     1381 atatcagcat tccaaaagac cttaagctgg atggcgtttc cttgctgccc tggttgcaag
     1441 ataagaaaca aggcgagcca cataaaaatc tgacctggat aacctcttat tctcactggt
     1501 ttgatgagga aaatattcca ttctgggata attaccacaa atttgtccgt catcagtcag
     1561 acgattaccc gcataacccc aacactgagg acttaagcca attctcttat acggtgagaa
     1621 ataacgatta ttcgcttgtc tatacagtag aaaacaatca gttaggtcta tacaaactga
     1681 cggatctaca gcaaaaagat aaccttgccg ccgccaatcc gcaggtcgtt aaagagatgc
     1741 aaggcgtggt aagagagttt atcgacagca gccagccacc gcttagcgag gtaaatcagg
     1801 agaagtttaa caatatcaag aaagcactaa gcgaagcgaa ataactaaac cttcatgcgg
     1861 cggaattttc cgccgcctta ttgagcgaga tagcgatgca cgttacagcc aagccctcca
     1921 gttttcaatg taatctcaaa tgtgattact gtttttacct tgaaaaagag tcgcagttta
     1981 ctcatgaaaa atggatggat gacagcactc tgaaagagtt catcaaacaa tatatcgcag
     2041 cgtctggcaa tctggtctat tttacctggc aaggcggtga acccactctg gctggcctgg
     2101 attttttccg taaagttatt cactatcaac aacgctatgc aggccaaaaa cgtattttta
     2161 atgcattaca aacgaatggc attttattga ataatgaatg gtgtgccttt ctcaaagaac
     2221 atgaatttct ggttggtatc tcgatcgatg gcccccagga gttacatgac cgttacagac
     2281 gcagtaattc aggtaacggt acttttgcaa aagtgatagc agccatcgag cgtctgaaat
     2341 catatcaagt agagtttaat acgttaaccg tcattaataa cgttaatgtc cattaccctc
     2401 ttgaggttta tcatttttta aaatctatcg gcagtaaaca tatgcaattt atcgaattgc
     2461 tagaaaccgg gacgccgaat attgatttca gtggtcatag tgagaacaca ttccgtatca
     2521 ttgatttttc tgtgcctccc acggcttatg gcaagtttat gtcaaccatt tttatgcaat
     2581 gggttaaaaa cgatgtgggt gaaattttca tccgtcagtt tgaaagcttt gtcagccgtt
     2641 ttttggggaa tgggcatacc agttgtattt tccaggagtc ctgcaaggat aatctggttg
     2701 ttgaaagtaa tggagatatt tatgaatgcg accattttgt ctatccacag tacaaaattg
     2761 gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa
     2821 aacggatttc agcgaaatgt ctgaaatgtg catataaacc tatctgcaat ggcggttgtc
     2881 ctaagcatcg tattactaaa gtaaacaatg agactgtttc ttatttttgc gaaggttata
     2941 aaatcctttt ttcaaccatg gtaccttata tgaacgccat ggtggagtta gctaagaaca
     3001 gagtaccgct ttaccacatt atggatgttg caaaacaaat ggagaataat taataacgct
     3061 ctcttagctc cagtcttagc ctgcttctta tgaccttaac aaggcacccg acataaattc
     3121 ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa tcctggctta cgtggtatgc
     3181 atcagggcat tagcaatatc ct
//
LOCUS       DAPGWD010000261         3188 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPGWD010000261 DAPGWD010000000
VERSION     DAPGWD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.263"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="RHX88_004356"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="RHX88_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDV2232954.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="RHX88_004357"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="RHX88_004357"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDV2232955.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="RHX88_004358"
     CDS             1252..1521
                     /locus_tag="RHX88_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDV2232956.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="RHX88_004359"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="RHX88_004359"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDV2232957.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..>3188
                     /gene="dsbA"
                     /locus_tag="RHX88_004360"
     CDS             2601..>3188
                     /gene="dsbA"
                     /locus_tag="RHX88_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDV2232958.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQ"
BASE COUNT          798 a          770 c          782 g          838 t
ORIGIN      
        1 aggccatccg tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
       61 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      361 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      541 gtttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaataa atgcatcttt
      661 attatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      721 ttctcctgct tgcagatatt ccagtaataa aggctcaatt gcgcggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagccgg gctgctaaat cctcgggaat ataaggagta tcgcacggac aaaacaaaaa
      901 ccactcgcct gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatggcgatt
     1021 ggcattaacc acgacgtgag agagctgcgt cataagcgcg tcagcgacat gttgccataa
     1081 tggtttgccg tttaattcaa gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1321 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgtggcgtaa ttaaagagta aaagcttgta agcggtgcca ccaaaatcat cgtgaaatga
     1561 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga tgctctattt gagcaaggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagttggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2041 tctgaatgaa tatctcattg agccacgcaa gctgtttgag gacgctacac tgatgccttc
     2101 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcaca
     2161 ctggcgggaa gacttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2281 ggatttgtgg atgttgctca atggcgataa agccgagcag cggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2461 cgcgttcccg aaaaatttcc cgtggttaac cggggaagat tactggctgc gacaaacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttacaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgtccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt gactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3061 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggtgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gcatggatac cagcaatatg gatgtttttg
     3181 ttcagcag
//
LOCUS       DAPGWD010000262         3130 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPGWD010000262 DAPGWD010000000
VERSION     DAPGWD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.264"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            651..881
                     /locus_tag="RHX88_004361"
     CDS             651..881
                     /locus_tag="RHX88_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232959.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRCRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            933..2294
                     /locus_tag="RHX88_004362"
     CDS             933..2294
                     /locus_tag="RHX88_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDV2232960.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDSETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRTRRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2342..2905
                     /locus_tag="RHX88_004363"
     CDS             2342..2905
                     /locus_tag="RHX88_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV2232961.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(2990..>3130)
                     /locus_tag="RHX88_004364"
     CDS             complement(2990..>3130)
                     /locus_tag="RHX88_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
                     /protein_id="HDV2232962.1"
                     /translation="RKKKNSSSSSSSSSSRNNNIISNRQMTGMKKQKPPSERPRNERG
                     GA"
BASE COUNT          725 a          871 c          913 g          621 t
ORIGIN      
        1 agcgaccgtt ccggtcgcgc cacccttgtc gcgccgtggc ctgcgctccc ggcatcccgg
       61 caaaaggccg ccgggatgta aggggcaacg ccgtttcgcg cacgcgctct ccgtcgttgc
      121 ggcgggtccc tgctcattgc ctgccggtgc cacacacccg tatgagtccc tgtcgccggg
      181 ggctgataca gccacaacct gccgcactga ctgtccaggg taaatgaaca gcccacaaaa
      241 ttttttaccc aaaacccggt aaaaaatttt gcgttctgcc caggacaggt gcgtcaggcc
      301 gtggcagtga tgccccttgc ggcgaaggtt ctcaaacggg tgaagttcat accacaggga
      361 atacagcagc aggttcccgc gaaacgtcaa aaccgcggct cccgaaactc aacgggtgat
      421 gttctttaaa aatcaggatt tatttcaggc tccggcaata cgcatttatg tgttctgtca
      481 gtcatccggc tggcaggtaa tgagaacgca ggaacagaag gaggaaaaca ccggagaacg
      541 tcagacgcag atatgaaaaa agcaggtcag ggacctgctt aatccacacc gcagagactg
      601 cacgaacagt ctttacggtt tcattaatta acgtcataag aggctaaatt atgacagtaa
      661 gtattgtttc accttccgca gcggctgtaa agccccgccg tcatccccgt tgcagacgcg
      721 aagacatccc cgcgccggaa attgaccccg tgctgaaagc cttcgggcga cacattgccc
      781 ggagttttca ccgggggcgt ggtgtacaca tccccgccat gaaaaacacg gccttcgggc
      841 aggtgctccg cacactcgaa ctgaaacgcg cttttaactg atgaggtgag ccgccccgcg
      901 aggggcggca acagacggaa ggagaacatt ttatgacatt agcagcaatc accatgaccg
      961 ccccggaagc cgccagccct gtgcagatgt accgcgcgac ctactcaccg gatgacaaca
     1021 aactgcgcct gtatgccgtg tcacgtcttg actcggagac gtataaaaaa gtgcatgatg
     1081 ccggtttccg ctgggcacca aaacaggcgc tgtttgtcgc gccagcctgg acaccgggcc
     1141 gggaagacgt gctcctctca cttgccggag agattgagga tgaagacagc acgctcgctg
     1201 aacgtcagga agcacgggcg gagcggttta ccggatacag cggaaagcgg gccagtgaat
     1261 ccgcacaggc acttgatgaa gtggaaagac tggccgcgat gatcccgccc ggtcagccca
     1321 ttcttgtggg gcatcacagc gaacgccgca cccgtcgtga tgcgcagcgt attgaaaacg
     1381 gcatgaaacg agccgtgatg ctctttgaac gtgcggaata ctgggaagag cgggcgcggt
     1441 cggcactgct tcacgcgaag tataaagaac gtccggacgt tcgctggcgt cgtatcaaaa
     1501 aaatcgaagc tgatttgcgc aaggctgaaa agaccatcgc gcagtcgcag aaatatctga
     1561 cgatgtggcg ggctgaatcg ctggatctga atatggcaaa actcatcagc agtcatgacc
     1621 atatcagcgc ctgtttcccg ctggatacgt atccgcgccc ggcagaaaaa agccagtatg
     1681 aaggaagtcg atcgttatgg tcggccctgg atgatgacat catcaccacg gagcaggccc
     1741 gcgaaattgc gatccgctgt catgaacggc agattcagca tcaacaacgc tgggttaacc
     1801 actatcagaa ccgcctgatc tatgagcgtg ccatgctgga cgaaagcggc ggcgtggtta
     1861 cccggacaca ggattttgag ccgggcggac aggttttcag ccggggcgag tggctgacca
     1921 tcatccgtgt gaacaaaagc aacggggcgg tgagttcagt cacaacgcca aattacagtt
     1981 ttctcgggta cagcggcacg atgaaagtga cgcccgatcg catcacggac tacaaagcac
     2041 catcggcaga agaggcagcc gtcgccagtc aggccgcgaa gcgtccgccg gtagtcaact
     2101 atccggggga aggtttccgg gaaatgacaa aggcacagtg ggccgccctg ccccgggact
     2161 gtaaggccgt gcgcagtgtg gcagaagcag aagaccacgg ggcataccgg taccgccgca
     2221 caatggacaa taatttccgc ctggtgaatg tgtatatcac cgacatgaaa atcacggaaa
     2281 tcccacagaa ataaggtata tccccccggg aaaatccccg gaggatgacg gagataatga
     2341 tatgcacagt cagttaagag aacgtatccg gctgatgcgc gcaaggctgg ataacgccgc
     2401 gccggttgct gaaatccggg ctgaatctca gctttttgtg actcctgccc cggtctgtga
     2461 tcgcctggtg atgctggcgg agatcagcaa ccgtgatcac attctggaac cctctgccgg
     2521 caccggggcc atactgcggg cgattcggga taccgcgccg ggggccatgt gtgatgcggt
     2581 ggaaatcaac agcgggctgg tccggtatct gcgggaaaat tttaacggtg tcagggttca
     2641 gtgtggcgac ttcatggaat ggcagccagt gcagtattac agccgggtta tcatgaatcc
     2701 gccgttcagc cacgggcagg atatccggca tatcctgcgg gccttttccc tgttgcgtcc
     2761 gggtggcgtg ctggtcgccg tctgtctcaa cggaccacgc cagcaggaga agctgttacc
     2821 gttttctgac gtccgcgagg agctgccgcg cggcacgttt gcttataccg atgtcccgac
     2881 gatgattatt cgtctgcgtg cctgatgaca cagggccggc gggaggataa atgcctgtct
     2941 gcccgcgccg ggaaaaaacg cgcgccggtg gcgctgaaag gggccgcgct cacgcgccgc
     3001 ccctttcatt acgcggacgt tctgatgggg gcttctgttt cttcatgcct gtcatttgtc
     3061 tgttactgat gatgttgttg ttcctgctac tgctactgct actgctactg ctactgttct
     3121 tctttttcct
//
LOCUS       DAPGWD010000263         3114 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPGWD010000263 DAPGWD010000000
VERSION     DAPGWD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.266"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2501)
                     /locus_tag="RHX88_004365"
     CDS             complement(<1..2501)
                     /locus_tag="RHX88_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDV2232963.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASAKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGAD
                     IPGKDTFTKNIGACRAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGN
                     NKIITDTGCGNIHLAGVVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYS
                     PGWRRDYNSRNKPTASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVY
                     NNGYPTTYGNIIHLKGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQL
                     YTSAHPPAEFYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRG
                     WTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLG"
     gene            complement(2566..>3114)
                     /locus_tag="RHX88_004366"
     CDS             complement(2566..>3114)
                     /locus_tag="RHX88_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDV2232964.1"
                     /translation="SGAPAWASEHQSTLSAGYLHASTNVSGSDDLNGINVKYRYEFTD
                     SLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAMAGV
                     AYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDIA
                     YEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          568 a          920 c          832 g          794 t
ORIGIN      
        1 cccaaatccg tactggatgc gctggcgctg tgggtgtgcg atttaatgcc gtcctgttcc
       61 tgagacaata cggcccgacc actggcaggt ttgcccttaa tcgtccagcc acgcatatca
      121 gggatcacgc ctgacggata agcggctgca agtttcgggt atgcagattt gtcaaaagtc
      181 tgcccctgca tcagggcata accagacgga acggtatctg atggccacgg gattggtgca
      241 ccgactggat aaaactctgc aggaggatga gccgaggtgt aaagctgcgc ccacggcgac
      301 cagtttgcgt cggtcgtatc ccgtcgtgaa cgaataaatg ccggagcatg agcaccgctt
      361 gtaccactcc agccgatgag taactcacct tcgccaacgg ctgtcatccc tttcaggtga
      421 atgatatttc catacgttgt tggatatccg ttgttataca cctcgtataa ctcaagacct
      481 gctgccccct gcgtattgtc tgtcagcgcg gctacccgac cttttgaagc cagattaact
      541 gatgatactg ctgttccacc tgacggtagc gccccgatct ctgatgccgt tggcttattt
      601 ctggagttat agtcccttcg ccagccaggt gaataatctg ttccgtgatt aatataggta
      661 aactgggcgt tggttgttcc gccaccgctg gatgtggtcg gagtggtaat gcggatcgtc
      721 atcgctgact ttatccccat tacttcaatg acaactccgg cgagatgaat attaccgcag
      781 ccagtatcag taatgatttt attattgcca taagaccagg aacccttgca catccagtat
      841 ggatggttaa atgctccctg agaatccagc cactcgataa actgtgcagt cgtccagttt
      901 cctgttgttg tgcttactga cccaccgaag gcacggcagg caccaatatt tttcgtaaag
      961 gtgtctttgc cagggatatc cgcaccgttc tgatctttct gcagacgttt ctcagcattg
     1021 tcattggctg cttttactgc ctttggcgtt gccgccagcg tttcagacgt gctgttggtc
     1081 gcgctgctta gctgtactat cccctttttc gtcgtgctcg catcctcaag cgccacggcg
     1141 gatgcaatat cctctgcccg ttttgccgct gtctcagcgc gcgttgctgc agattccgcc
     1201 gtacttttgc tctgagctgc tgccttcgca ctaccagcag cctctgtcgc cttcgtggat
     1261 gccgtcgtgg cgctgccctt cgctgctgac gcctgtctgg tcgcctcatc ttttgaagca
     1321 gacgccgatg atgccgatga cgccgccgaa ctggcggacg atgccgctgc cgtttttgag
     1381 gattctgcac gggtttccga cgctttcgcg ttcgtttcgg atgtcttcgc tgcggaagct
     1441 gacctcgctg ctgcgctggc ctgctcagtg gcttcgccag ccttcgttgt ggctgttgaa
     1501 gcggatgatg cggcgctttc tgccgatttt ccggcggcgg tggcactggc tgaggcctgc
     1561 ccggcacttg ttgacgcggc actggcagat gacgcagccg ctgtttttga gcctgccgca
     1621 gctgaggcgc tctgtcccgc tgccgtctca gaagacctgg cgttcgtctc ggacgttttt
     1681 gccgccttcg cggaatttcc tgccgccgtt gccgaggaag ctgcgctact ggcgctcaag
     1741 gatgcgttcg tttctgatga tttcgctgcc tcttttgagg ccgccgcatc ccgggctgag
     1801 gtggctgctt ctgacgcttt cgtggtcgcg gtggatgcag aagtggctgc tgattgttgt
     1861 gacgctgccg cattcgtttc tgacgttttc gccgcaccgg cactggtagc cgccgcgctt
     1921 tttgaggact ctgcagcggc agcacttttt gatgcttcag tggcctttgc tgatgccgtt
     1981 cctgcgctgg aagacgctga ctgagccgac gacgcggcct gtccggctga cgtgctggct
     2041 gcgcgtgctg agcctgcagc atcagtcgca tgggttgccg cctcacgggc tgatgtgctg
     2101 gcatcgctgg ctgacttctt cgcggctgct gtgttctgtg ccaccgcgga cgcgttacgc
     2161 gccacctctt ccaccatcag ttcaaaacgt cgcagtgcct ccggacgggc atcatcctcc
     2221 gtcatggcac cgagaaaatc attcagcgta ccgggttgag aatcttcata cacggtgatg
     2281 gtcccggcat gtgacggcgg gaatccctcc accaacagaa taacgctgta ctgaccgtac
     2341 tcaacgtcca tactgtaacg cccggcttca tccggatttt ctgaggccag cgtgttcacc
     2401 accaccgtgg tactgttacg ttttgctttc agctggattg tgcagttctg taccggtttt
     2461 cctgtgccgt ctttcagtac acctgaaatc tttactgcca tattcacccc acaaaaaagc
     2521 ccgcctgaac cggcgggctg tcataacact gtgttacctg gctaatcaga atttataacc
     2581 gacacccacg atgaaaccgt cagtgcgcca gtcgccactg ccggagcctt cataagcaat
     2641 atcaatggcc acggattcgg tcgggttaaa ctgcacgcca gccccccacg ccagagacgt
     2701 gttgctgtgg cgaccgtcat cacttccggt cagcacatcg tgcgttttcc ccttgttgtc
     2761 agttacgcgg agataatccc cggagaaagt cgacacacgg ctgtaagcca cacccgccat
     2821 cgcatacgcg ctgaaccatt cattcacgcg tacagacggc cccgccatca cgctgaacca
     2881 gcggttacgc acggaatctt catgccagcg ggtatcgctg taacgggtaa gctggcgatt
     2941 cttgtctcct gcatagctga atgacgtcac cattcccagc gagtccgtaa actcataacg
     3001 gtatttcacg ttaatcccgt tcagatcatc gctgccggaa acgttcgtcg aggcatgaag
     3061 atagcccgcg ctcagcgtgg actgatgttc agacgcccat gcaggcgcac cgga
//
LOCUS       DAPGWD010000264         3082 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPGWD010000264 DAPGWD010000000
VERSION     DAPGWD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.267"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            28..1623
                     /locus_tag="RHX88_004367"
     CDS             28..1623
                     /locus_tag="RHX88_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV2232965.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            1637..2788
                     /locus_tag="RHX88_004368"
     CDS             1637..2788
                     /locus_tag="RHX88_004368"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDV2232966.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(2831..>3082)
                     /locus_tag="RHX88_004369"
     CDS             complement(2831..>3082)
                     /locus_tag="RHX88_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2232967.1"
                     /translation="YRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFA
                     KLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
BASE COUNT          824 a          713 c          796 g          749 t
ORIGIN      
        1 aaatcacatc tgaatcagga attaacaatg aaacctgtaa aaccacctcg tattaatgga
       61 cgagtgccgg tcctgtcggc acaggaagcg gtgaattata ttcccgacga agcaacactt
      121 tgtgtgttag gcgctggcgg cggtattctg gaagccacca cgttaattac agctcttgct
      181 gataaatata aacagactca aacgccacgt aatttatcga ttattagtcc gacagggctt
      241 ggcgatcgcg ccgaccgtgg tattagtcct ctggcgcaag aaggtctggt gaaatgggca
      301 ttatgtggtc actggggaca atcgccgcgt atttctgaac tcgcagaaca aaataaaatt
      361 attgcttata actatccaca aggtgtactt acacaaacct tacgcgccgc cgcagcccac
      421 cagcctggta ttattagtga tattggcatc gggacatttg tcgatccacg ccagcaaggc
      481 ggcaaactga atgaagtcac gaaagaagac ctgattaaac tggtcgagtt tgataacaaa
      541 gaatatctct attacaaagc gattgcgcca gatatcgcct tcattcgcgc taccacctgc
      601 gacagtgaag gctacgccac ttttgaagat gaggtgatgt atctcgacgc attggttatt
      661 gcccaggcgg tgcacaataa cggcggtatt gtgatgatgc aggtgcagaa aatggttaag
      721 aaagccacgc tgcatcctaa atctgtccgt attccgggtt atctggtgga tattgtggtg
      781 gtcgatccgg atcaaagcca actgtatggc ggtgcgccgg ttaaccgttt tatttctggt
      841 gacttcaccc ttgatgacag caccaaactt agcctgcccc taaaccaacg taaattagtt
      901 gcgcggcgtg cattattcga aatgcgtaaa ggcgcggtgg ggaatgtcgg cgtcggtatt
      961 gctgacggta ttggcctggt cgcccgggaa gaaggttgtg ctgatgactt tattctgacg
     1021 gtagaaacag gtccgattgg cggaattacc tcacagggta tcgcctttgg cgcgaacgtg
     1081 aatacccgcg ccattctgga tatgacgtcc cagtttgatt tttatcacgg tggtggtctg
     1141 gatgtttgtt atttgagttt tgctgaagtc gaccagcacg gtaacgtcgg cgtgcataaa
     1201 ttcaatggca aaatcatggg taccggtgga tttattgata tcagtgccac ctcgaagaaa
     1261 attattttct gcggcacgtt aactgcgggc agtttaaaaa cagaaattgc cgatggcaaa
     1321 ttgcatatcg tccaggaagg acgggtaaat aaatttattc gggaactacc ggaaattact
     1381 tttagcggaa aaatcgcgct cgaacgggga ctggatgttc gttacatcac cgaacgtgct
     1441 gtattcacgc tgaaagaaga cggcctgcat ttaatcgaaa tcgcccctgg cgtcgattta
     1501 caaaaagata ttctcgacaa aatggacttc accccagtga tttcgccaga actcaaactg
     1561 atggacgaaa gattatttat cgatgcggcg atgggttttg tcctgcctga agcggctcat
     1621 taacaggagt ataatgatgg atttttcttt aactgaagaa caagaactgc tgctggccag
     1681 tattcgcgaa ctgattacga ctaactttcc ggaagagtat ttccgcacct gcgatcaaaa
     1741 cgggacatat ccgcgtgagt ttatgcgggc gctggcggat aacggtattt ccatgcttgg
     1801 cgtgccggaa gaatttggtg gtatccctgc ggattacgtc acccaaatgc tggcgctgat
     1861 ggaagtgtca aaatgcggtg ctccggcatt tttaattacc aacggtcaat gtattcacag
     1921 tatgcgccgt ttcggttctg cagagcagct acgtaaaacg gcagagagca ccctggaaac
     1981 gggtgatccc gcctatgccc tggcgttgac ggaaccaggt gctggctcag ataacaacag
     2041 tgccactacc acttacacgc gtaaaaacgg caaggtctac atcaacggac aaaaaacctt
     2101 tattaccggt gcgaaagagt acccgtatat gctggtgctg gcgcgcgatc cgcaaccgaa
     2161 agatcccaaa aaagccttca ccctgtggtg ggtcgactcc agtaagcccg gcattaagat
     2221 taacccgctg cataaaatcg gctggcatat gctcagcacc tgcgaagtct atctcgacaa
     2281 cgtggaggtt gaagagagcg acatggtggg cgaagaagga atgggtttcc tcaatgtgat
     2341 gtacaacttt gagatggagc gcctgatcaa cgccgcgcgc agcaccggct ttgccgaatg
     2401 cgcctttgaa gatgccgccc gctatgccaa ccaacgtatc gcttttggta agcccattgg
     2461 tcataaccag atgatccagg aaaaactggc gctgatggcg attaagatcg acaacatgcg
     2521 caacatggtg ctgaaagtgg catggcaagc cgatcagcat cagtcactgc gcaccagcgc
     2581 ggcgctggca aaactgtact gtgcacgtac cgcaatggaa gtcattgatg atgcgattca
     2641 aatcatgggc ggtctgggct ataccgatga ggcgcgcgtc tcccgcttct ggcgtgatgt
     2701 ccgttgtgaa cgtatcggcg gcggtacaga cgaaattatg atttacgtag cgggtcggca
     2761 gatcctgaaa gactatcaga acaaataatc tgcaggcggc gcagcttctt aacaaactgc
     2821 gccgccagat ttacccggca agacttaccg gttgaggaaa ttcagatagg atttcttgtt
     2881 ccgcaaaaca gtttttaaat tgcctgcggt aatcgctggg cgaacaaccg acatggcgca
     2941 aaaacagttt ggcaaagtga tcgacatttt cataacccac acgccaggag atctctgcct
     3001 gtgataattc agtattagtg agtgaccatt tcgcttccgt catacgccgt tgaataacat
     3061 agttaatagg ggaaatacga ta
//
LOCUS       DAPGWD010000265         3068 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPGWD010000265 DAPGWD010000000
VERSION     DAPGWD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.268"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(267..2528)
                     /gene="katE"
                     /locus_tag="RHX88_004370"
     CDS             complement(267..2528)
                     /gene="katE"
                     /locus_tag="RHX88_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDV2232968.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNSLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            2711..2974
                     /gene="cedA"
                     /locus_tag="RHX88_004371"
     CDS             2711..2974
                     /gene="cedA"
                     /locus_tag="RHX88_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDV2232969.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT          749 a          754 c          800 g          765 t
ORIGIN      
        1 atgcgtcacg gcataatccg gtagcggcgt aaaaatcgcg gaagggatga aaaaaacagc
       61 gcctgacggc gcagtgtctg gcatgcctgc aatccgggaa accggaccag gaaaaaactt
      121 gcaggccata acagtatcta ccaataaact gcaatacatt gaatttatac gactttgtag
      181 gccggataag gcgttcacgc cgcatccggc ataaacaaag cgcactatgt aaatcatttg
      241 aggcggcgca attgcgccgc ctcccatcag gcaggaattt tgtcaatctt aggaatgcgt
      301 gaccacacgc ggtgtgctgc catcagcgtt agcagttcat ccataaaact accgtcagcg
      361 ctgtcagctt ccacaatccc ttcttcaccc tggtcagcga ccttgattgt tgctttaaac
      421 ttgcgcgcgt ctcccgccag cgcaatcggt ttaaggtgtt tgtaggcttc catcaggtag
      481 tagttggcat cgccgttgtc agcgatatcc gcgatattgc cgcaagggac aatgaccgca
      541 tcgaccgtca gcgaaggtgc accggcaaag gtagcggcta taggcaacac cgtaccgtca
      601 tccgcagtca cttcacccat tcgggagtag agcagtttgg catgaacgcc tttggccttc
      661 agggccttga gaatggccag aaggtctgcc gatctcactt catcattaag taaaatcgct
      721 accacgcgac ctttcacatc accgtcagga atggcgtaca aacttaagga tggatccttt
      781 ttcagactgt tgacgtccgg aggtggggtg atattcagct ggtcgtcagt cagttcgata
      841 ccgagatttt tcgccaccgc ctgggccaga gtgagatcaa tatgcgccag ctggtcaaca
      901 acgcgctcac gaatatacgg acgaacgact ttgcttaact caaaactgaa accatcgaca
      961 atatggcgct gctcgaatgg cgtctgactt agccagaaca gacgcggatg ggaataatat
     1021 tcgccaaacg atggactgcg ctcacgaact ttatttcctt ccacgcgttc ctgatatgat
     1081 tcaaaaccgc cgcgtttcgg tcccggcggt gtttcgcgcg gccagttatc gttgatcgag
     1141 ttcggttcgt aattcgccgg gttagtgtcg atccccatac gatgcatgcc atcacgctgg
     1201 aagttgtggt aagggcaggt cggacggtta atcggaatct catggaaatt cggcccacca
     1261 agacgactga tttgtgtatc ggtataggag aacaaacgtc cctgcaacag cggatcgttg
     1321 gtgaagtcca gaccaggaac aatatgacct ggatggaatg ctgcttgttc gttttcggca
     1381 aagaagttat ccggattgcg attgagcacc attttgccga cacgctgaac gggcaccagt
     1441 tcttccggga tcagtttggt tggatcgaga agatcgaagt cgaacttgaa ttcatcttct
     1501 tcaggaatca actggaagcc cagttcgtat tccggaaaat cgcctgcttc aatggcttcc
     1561 cacaactcgc ggcggtggaa gtccgggtca cgtccggtga gtttttgtgc ttcatcccaa
     1621 acgagtgagg ctttacctgc cagtggtttc cagtggaaac gtacaaacgt tgccttccct
     1681 tcggcattaa tcaggcggaa ggtgtgaata ccgaagcctt ccatggtgcg gtaactgcgg
     1741 gggatgccgc gatccgacat cgcccacatc acgttgtgca gagtttcagg ttgcagagaa
     1801 acataatccc agaaagtatc gtgggcactt tgcccttgtg gaattgccca gtgcggttct
     1861 ggttttaccg catgaacaaa atcggggaat ttatgcgcat cctggataaa gaagattggc
     1921 gtgttattgc caacgaggtc aaaaataccc tcttcagtat agaacttggt ggcaaaacca
     1981 cggatatcac gcacggtatc ggcagagcca gcgccaccct gaacggtaga gaacctaaca
     2041 aatactgggg tgattttgtt cggatctgag aggaaatccg ctttggtgat atcgcttaag
     2101 cttttatatg gctggaaata accgtgagct gctgatccgc gtgcatgaac aatacgttcc
     2161 gggatgcgct catggtcaaa gtgggtgatt ttctcgcgca gaataaaatc ttccagcagc
     2221 gttggaccac ggttaccggc acgcagtgag ttttgatcat cggcgatgcg cacgccctga
     2281 ttagtggtca gcgcataatt ttcactgcct ttgcgtacgt cttccagaga attaagtttt
     2341 tcgttacgtg tatcaggggc tttcaggctc ccaggggcgg taggttgtgc acctggcggt
     2401 gttggttcag ccgctggacg atgagatccg tcctcaggtg ccagtgagtc catccccggt
     2461 ttcgcttcgc tggaatcgtg tagtggtgac tggtgctgat gtgggttctt ttcgttatgt
     2521 tgcgacattg aactcgtctc cttaatttat tactgaaagg gccgctgttt tggtcaaaaa
     2581 accctctaac tatagaccat cagccagatc ccgcttcgga gactattctg gaaacggtgt
     2641 tatgaaatac acgcaaagtg ggacgtacag gggctaaatc ggctaaacta cagtttttct
     2701 gatttttaat atgcgtttag tgaagccagt aatgaagaaa ccgctccgcc agcaaaaccg
     2761 ccagattatt agctatgtcc cacgcacgga acccgcgccg ccagaacatg cgataaagat
     2821 ggattcgttt cgtgatgtct ggatgctgcg tggcaaatat gttgcgttcg tactgatggg
     2881 agagtcattt ctgcgctcac cggcgtttac tgtgcctgaa tccgcccagc gttgggcaaa
     2941 tcagatccgc caggaagggg aagtgactga gtaaagatta cgtacctccc ggttttgccg
     3001 ggagttacat gacctgaaat tctgtatttc attgctacat tcattcaaac caccagtaac
     3061 aaaaagct
//
LOCUS       DAPGWD010000266         3065 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPGWD010000266 DAPGWD010000000
VERSION     DAPGWD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.269"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(46..1905)
                     /gene="gss"
                     /locus_tag="RHX88_004372"
     CDS             complement(46..1905)
                     /gene="gss"
                     /locus_tag="RHX88_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDV2232970.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2110..2976
                     /gene="yghU"
                     /locus_tag="RHX88_004373"
     CDS             2110..2976
                     /gene="yghU"
                     /locus_tag="RHX88_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDV2232971.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          724 a          824 c          773 g          744 t
ORIGIN      
        1 tactgattat tcttcataat cagtacgggg ttgctacaac atgtattact ttttcaccac
       61 aattaacggt tcaatatcac tctctttttt gatgaccagt gattcatcac cgcgcaaaca
      121 cgtcccaccg tagttgccgc ccacggtgaa ggtacatacc tgaatgtatt taccgtccac
      181 tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt taccgctggt
      241 tttgtccagc acctcttcat gatggctgac caggtcgata ttgctaccac agcgaccggc
      301 aatcggtttc actgcgtaac ctgttttcac caattcatca ttaacagtga aatcggtatc
      361 cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc
      421 ggggatcacc gtccacagcg gctcaaagac tagcacttcc gggcgcagca atacgtcgat
      481 aagacgcact tcgttttgcg gatgaccggt acggattggc accgcagcaa actcacggtc
      541 gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc acacgcagtt
      601 aaccagtcgc ccttccccat caatcagttg cccggcagca tcccagccca gttcatccaa
      661 tccacgcaag atacgcgttt caaagcccgc ctggtgcagc gcctgctcca taaactgcgc
      721 gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac gtgcacgact
      781 gtgtttccag gcaccagcca attcgttaat cagcccttcc gccggattga agccgttgcc
      841 tttatagccc tgctccgccc aacgttcgag gatcaagcca gcttcggtat gacaggaggc
      901 ggagtcggcg ttgtattcgt aaaccttcag gccacgctca tccatgcaga aatccatacg
      961 accagtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat
     1021 tttcgggatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt cggtcgcgtg
     1081 aagatacatc agatgcagct cgttggtggc tttaattagc tcctgctcgg cactctcggt
     1141 aatggtgtag taatgataag gatcctgatt gatcacctga ccgttggcct gcacataggc
     1201 gttttgcagc ggatcttttt catccagcca tttaccgtca aactggcctt tgttttccag
     1261 gcgcgctccg ctgattttca gcagctcgcc tgcaatttcc ggctgcggta agctgtattc
     1321 agtatcttcc gtctggatca tccagcccag aatggtggtg tcatcaaaag tgtctttcag
     1381 ggtatagcag ccgttttcga ccaccatctc cagctcacgc gtccactgtt gcccttgcgg
     1441 caacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat gcaattgggt
     1501 aatgatggcg acatggccag tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc
     1561 acccgcgacc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc
     1621 attaaccact tctcgcagga agcgcagcga gaaaatctcc cacgccatac ccacgtcagt
     1681 aaagaccaca ccgtaattca gaaagagaaa acggcgagca aattcaacgc attgccactt
     1741 gtggcccata tattcgtcgt cgatatagct acggaatacg gcgtcatctt cgtattcctg
     1801 cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg cgtagcccaa
     1861 taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt tacctttaac
     1921 aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca gcgacattaa
     1981 ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa agggttaaaa
     2041 aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc actcagaagg
     2101 caactcacta tgacagacaa tacttatcag cccgcgaaag tctggacgtg ggataaatcc
     2161 gctggcggcg cgttcgccaa tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg
     2221 ctgcccgttg gcaaacaccc attgcaactt tattcgctgg gaacgccgaa cggtcagaaa
     2281 gtaacgatta tgcttgagga gctgctggcg ctgggcgtta ctggtgcaga gtacgacgcc
     2341 tggctgattc gtattggcga tggcgatcaa ttctccagcg gctttgtcga agtgaaccca
     2401 aactcgaaga tcccggcgct gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa
     2461 tctggttcga tcctgcttta tctggcggag aaatttggct acttcctgcc gcaggatttg
     2521 gcaaagcgta ctgaaacgat gaactggctg ttctggttac agggcgcggc accgttcctc
     2581 ggcggtggtt ttggtcactt ttaccattac gcgccgatga aaattgagta cgccatcaac
     2641 cgctttacca tggaagccaa acgtctgctc gacgtgctgg ataagcaact ggcgcagcat
     2701 aagtttattg cgggcgatga gtacaccatt gcggatatgg cgatttggcc gtggtttggc
     2761 aatgtggtgt taggcggcgt gtatgatgcc gcagagtttc ttgatgcggg cagttataag
     2821 catgtacaac gctgggcgaa agaagtaggc gaacgtccgg cggtgaaacg tgggcgtatt
     2881 gttaaccgca ccaacggacc gctgaatgag cagttgcatg agcgccatga cgccagtgat
     2941 ttcgagacga atacggaaga taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc
     3001 catccaggcc ggataaggcg ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg
     3061 acgct
//
LOCUS       DAPGWD010000267         3055 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPGWD010000267 DAPGWD010000000
VERSION     DAPGWD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.270"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(324..482)
                     /locus_tag="RHX88_004374"
     CDS             complement(324..482)
                     /locus_tag="RHX88_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV2232972.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            complement(762..1481)
                     /locus_tag="RHX88_004375"
     CDS             complement(762..1481)
                     /locus_tag="RHX88_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDV2232973.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     DRLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            complement(1478..1912)
                     /gene="psiB"
                     /locus_tag="RHX88_004376"
     CDS             complement(1478..1912)
                     /gene="psiB"
                     /locus_tag="RHX88_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDV2232974.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(1967..>3055)
                     /locus_tag="RHX88_004377"
     CDS             complement(1967..>3055)
                     /locus_tag="RHX88_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232975.1"
                     /translation="EFLREAEGWGWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVGGISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGVAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIITALNEAGLSGAARDAEKMKKGDAAEHAEHHKKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
BASE COUNT          652 a          965 c          784 g          654 t
ORIGIN      
        1 gaaggtgcgc ccggcagaca ggacaacact gcagtagtca gaatcagagc aggaagaaaa
       61 agagaataat cacagggaag gtgatgtgat ttctgacagg ccataaccag agaaggcggg
      121 ggaaaagaac gggcgccgca ggcgcgcgtt catccccgtt cctgaaggga aacgaccttc
      181 ctgaagtcag gttgttcatg cccgtacaac atcagcaagg agaaaggggc taccggcgaa
      241 ccagcagccc ctttataaag gcgcttcagt agtcagacca gcatcagtcc tgaaaaggcg
      301 ggcctgcgcc cgcctccagg ttgctactta ccggattcgt aagccatgaa agccgccacc
      361 tccctgtgtc cgtctctgta acgaatctcg cacagcgatt ttcgtgtcag ataagtgaat
      421 atcaacagtg tgagacacac gatcaacaca caccagacaa gggaacttcg tggtagtttc
      481 atggccttct tctccttgcg caaagcgcgg taagaggcta tcctgatgtg gactagacat
      541 agggatgcct cgtggtggtt aatgaaaatt aacttactac ggggctatct tctttctgcc
      601 acacaacacg gcaacaaacc accttcacgt catgaggcag aaagcctcaa gcgccgggca
      661 catcatagcc catatacctg cacgctgacc acactcactt tccctgaaaa taatccgctc
      721 attcagaccg ttcacgggaa atccgtgtga ttgttgccgc atcacgctgc ctcccggagt
      781 ttgttcggca ccatccaccg ctccatctca cgaacagcat gaccaaccac ccttgccgcc
      841 ataccagcct ctgcgatgac ctgccagaat ggcatatcct gccactgcca gcgttccatt
      901 ccggccagtg acggaaaacg ctcgccccac tgccagaaca gggtttccag gtcatgctct
      961 gccacccggt cactccagcg tgcatgccag cttccgactg tttccggcga atggaatgcc
     1021 agttcgattt cagcctgagc gaccagcgcc tggtacgcac gggctttctg ctctgcctcc
     1081 ctcgcctgct ggcggctgta cttctgcatg accagcgcgg atttatggcg actgcgttcc
     1141 ctgacacgaa accgcaccga cgggaacaag cgatctgctg catcaccggg aaggggaagg
     1201 aggcagacct cgccctgtgt ttcaatcagc acatcgatgg catacagcca ctgaagacgg
     1261 ttgccgtgca gttcttctgc ggtcagggac ggcatgaaaa atcgcaggtc agacagcgat
     1321 atcctgcggc ttccgttcag acagcggaaa aacgcccccg catacggata ttcagccagc
     1381 gtgttaccct gatggcggcg tttttcggtc tccgccaccg cccgccatgc ggcctgttgc
     1441 tctgtgctga gaggaaccag tgcctgtgaa cgtgcgctca tgcctgacct ccttccgcca
     1501 tcaggatatg gatgatgtca ctgacgctgt acccctgcgc gtccagtgac gccgccagtg
     1561 ccagtgaatg gctgacggct tccggtgcaa accgccgctg tacctgaacc accgcaaacg
     1621 gctcaccacc ggcattcacc agaaccagca cccagacctg agagacatcg cccggtgaac
     1681 ataacgccag gtggaaacgt tcgtgggctg gcgtgaaacg aacctggacg gggaaaaagc
     1741 ccccgaactc actgccatac tcgccattca tcatccagtt agccggtgcg tccacttcac
     1801 gcatcacctc gtgagtgaga ttacggcgca tatcgcttcc cgcagcacgg atatcttcat
     1861 attcctgtgc gttcatggac tgcagggcat tcagggtcag ttcagttttc atcacggtat
     1921 ctcccttgct gccgccccgt ctccggcggg gcggtgtggt tgttcatcag gcggcatcag
     1981 ccaggttatc ggtgcgttca gtggcatctg tctgtggatg tggtgcacac atccagcctg
     2041 gaacccagcg gttgtctttc ttatggtgct ctgcatgttc agccgcatca cctttcttca
     2101 tcttctccgc gtcccgtgcg gcaccggaca gtcctgcctc attcagggct gtgataatct
     2161 gcggcttttt caggtgtccg aagaagtttg cttttgtcgg ctgccaccag tcgcgcatgt
     2221 ggaatccgat agcgctttcc agcgagtcaa gcggactgcg tgacgtgtga ccacactcac
     2281 gggtctggac accgttaagg ctgcatgcgg tgcagaaact gagcagggat aacagcacct
     2341 cctgactgag tgacaggaag gtcgtcatgt cccgtgacca tccctccggt aacagggcag
     2401 caagacgggc tttttctgcc atcagcgcca tgaatgcgac accttccttc cctgacggcg
     2461 catcgctggt cagcgaataa tgtttacatt ccaggctgat ttgtgccggt ttactgtacg
     2521 ctccgctgcc aaacacattc aggcagagcg tccatgccag cagtgccaga gatttgtccg
     2581 gttgctgcat aagcgccgcc tggactgcca gcgtgcgttc ggaagacatt ttcgtcagca
     2641 gtggcagact gattcccccc actgacgctt tctcctgcac ctgttccgtg cggtcagcgt
     2701 cgtcagccac gtcatcctca ctgcgcaact gcacaccacg ctggacacac acgttgccat
     2761 aacgccagga caccaccact ccacctccgg cacgcatctc cggcgtccac gctctgacct
     2821 tcgccatgca gcgcatcagc ttcatttctg cttccagcag gtcagattcc tcacactggt
     2881 tttccagcgc gtcgtaacga gccatcagtt cgttcagacg ttcttcttcc gcctccgtca
     2941 gcaccgcttc cggctccggc agacagcggt atgttccggc atcctcacgg cactcaccta
     3001 caggctccat gcgtccggca caccatcccc agccttcggc ttcccgaagg aactc
//
LOCUS       DAPGWD010000268         3008 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPGWD010000268 DAPGWD010000000
VERSION     DAPGWD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.271"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(98..1636)
                     /gene="aldB"
                     /locus_tag="RHX88_004378"
     CDS             complement(98..1636)
                     /gene="aldB"
                     /locus_tag="RHX88_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDV2232976.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(1801..2952)
                     /gene="yiaY"
                     /locus_tag="RHX88_004379"
     CDS             complement(1801..2952)
                     /gene="yiaY"
                     /locus_tag="RHX88_004379"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDV2232977.1"
                     /translation="MAASTFFIPSVNVIGADSLADAMNMMAEYGFRRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSIIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT          701 a          831 c          753 g          723 t
ORIGIN      
        1 acgccttatc cggcgtacgg tcaggtcccc gtaggcatga taagacgcgt aagcgtcgca
       61 tcaggcaatt aatacccgat gcgaccagct tcttacatca gaacagcccc aacggtttat
      121 ccgagtagct caccagcagg cacttggttt gctggtaatg ctcaagcatc atcttgtggg
      181 tttcgcgtcc aatgcctgat tgtttgtagc cgccaaacgc cgcatgtgcc gggtaagcgt
      241 gataacagtt ggtccacacg cgcccggcct gaatgccgcg ccccatctta taggccagat
      301 taccgttgcg gctccagacg cctgcgccca ggccatattg cgtatcgttc gccagctcca
      361 gcgcttcttc catcgttttg aaggtggtta ccgccagcac cgggccaaaa atctcctcct
      421 ggaacacgcg catattgttc tgaccaaaca gaatcgtcgg ttcgaggtag tagccgtctt
      481 tcagttcgcc ttccagcagc ttacgccgcc cgccggtgag cacatcagcg ccctcttttt
      541 taccgatatc aatgtagttg aggatggttt ccagttgccc gtgagatacc tgcgccccca
      601 tttgcgtcac gctgtcgagc gggttaccgc tgcgaatgct ttcgacacgg cggatggcgc
      661 gttccataaa gcgttcgtag attgactcct gcactaaggc acgactcgga caggtgcaaa
      721 cttcgccctg gttaaaggca aacagtgcaa agccttccag cgctttatcg aaaaaggcat
      781 cttcttcatc catcacatca gcaaagaaga tatttggcga cttaccgccc agctccagcg
      841 tcaccggaat aatgttttgc gttgcgtatt gcataatttg ttggcccact tccgttgagc
      901 cggtaaacgc cactttggcg atgcgtttcg aggtcgccag atattcgcca atttccccac
      961 cagcgccatt gaccacgttc accacgcccg gcggcagtaa atcaccgacg atttccatta
     1021 gcagcagtac agaaagcggg gtaagacgtg cgggtttcag caccacacag ttgcccgccg
     1081 ccagcgcggg agccattttc cagctcgcca tcagcagcgg gaagttccac gggataatct
     1141 gccccaccac gcctaacggt tcatggaaat gataggccac ggtttcgcta tcaacttcac
     1201 tgatcccacc ttcctgcgcc cgaatacacg aggcgaaata gcggaaatgg tcaatcgcca
     1261 gcggtacatc cgcagcactg gtttcgcgaa tgggtttgcc gttatcccag gtttcagctg
     1321 tcgctaacag ctcgaggttt tgttccattc gatcggcaat cttaaacaga atcgccgcgc
     1381 gatcctgcac cgaagtatgc gcccatttat ctttcacttt gtgcgcagca tccagcgcca
     1441 gatcgatgtc tcgtttgccc gaagacgcca cttcgcacag cagctgcccg gtcaccggcg
     1501 tcaggttctg gtaatactca ccatcggcag gggctaccca ttcgccgcca ataaagttgt
     1561 cgtagcgggc ttttaacttg atggggaaac catactcgcc gggcttaatc tgtgctgaag
     1621 ggggattatt ggtcatgatc gtctccttgc ggtgtgaggt ataacaaggg tagacgtgac
     1681 tggcgaaatc ttcgccagtc ggtaacagct ttacgacagc tatcacgaat ttacgggcaa
     1741 ggattacaga gatagttgaa aaaatggctg acaatttgcc agccgttgtg gaaatgatga
     1801 ttacatcgct gcgcgataaa tcgccacaat ttcttcgtga gttgcctgga tcgggttagt
     1861 aaagccacag gcatctttca gggcattagt cgccagaacc gcgaaatctt cttctttcac
     1921 gttcaggtcg cgtaggcctg ccgggatatc cactttcttc gccagttcac ggatggcgtt
     1981 aatgcaggct tcagcacctt ccgcgtcgtt tttacctgtc acgttcacgc ccattgcagc
     2041 ggcacagtca cgcagacgtg cagcggcgac tttgctgttg aatacctgaa catgcggcag
     2101 cagaacggcg ttacatacac cgtgtggcag gttgtagaaa ccgcccagct ggtgcgccat
     2161 cgcatgaaca taacccagag aagcattatt gaacgccata ccggcaagga actgggcata
     2221 agccattgct tcacgcgctt tcgcattact gccatcttca acggctaacg gcaggttttc
     2281 ggcaatcatg gtcacggctt tcagtgcaca agcgtcagtg atcggcgtgg cggcaataga
     2341 aacatacgct tcgatagcgt gcgttaaggc atccataccc gttgcggcgg tcagtgactt
     2401 cggcatacca atcatcagag aggagtcatt gacagaaagc agcggagtga catgtttatc
     2461 aacaatcgcc attttgatat gacgcgcttc gtcagtgatg atgcagaaac gggtcatttc
     2521 agaggccgta cccgccgtgg tattgatggc gatcatcggc agctgcggtt ttgcagagcg
     2581 gtcaacgcct tcgtaatcgc gaatatcgcc gccattggct gccaccagcg caataccttt
     2641 tgcgcagtcg tgcggagaac caccgcctaa ggagatcacg ctatcgcaat tattttcttt
     2701 aagtaatttc aaacctgcgg cgacgttttc cgtggtgggg ttaggttggg tgccatcata
     2761 aataatgcta aaaatattgc gttcttccag tgctttttgc acatcgcccg ccatacctaa
     2821 tttcgttaac atactgtcag tgacaatcaa tgtacggcga aacccatatt ctgccatcat
     2881 attcattgca tcagccaatg aatcagcgcc gatgacattc acagaaggaa taaaaaaggt
     2941 tgaagctgcc ataacacact cctgaaagtg taaaaaaggg cgggcagctt actgaggatt
     3001 ttcattcg
//
LOCUS       DAPGWD010000269         2886 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPGWD010000269 DAPGWD010000000
VERSION     DAPGWD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.272"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(119..322)
                     /locus_tag="RHX88_004380"
     CDS             complement(119..322)
                     /locus_tag="RHX88_004380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232978.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRD"
     gene            445..768
                     /locus_tag="RHX88_004381"
     CDS             445..768
                     /locus_tag="RHX88_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV2232979.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            774..1910
                     /locus_tag="RHX88_004382"
     CDS             774..1910
                     /locus_tag="RHX88_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2232980.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMVANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1907..2881)
                     /locus_tag="RHX88_004383"
     CDS             complement(1907..2881)
                     /locus_tag="RHX88_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2232981.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKYEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          732 a          658 c          713 g          783 t
ORIGIN      
        1 cgccgcatcc ggcataaaca aagcgcacgt tgttacaatt tacgttttaa acctacgtgc
       61 gcaataaccc agactacgcc aacagaagtg cgtttctggg gcgtgttttg ccaggagttt
      121 aatctcgcgt ttcgatggtg agcgggcaaa ccaggccgta aatcaccatt ggcgttccga
      181 ggccaatcag caataaacgg tagataattg aaggtttaat acgctttatc tgtactgtgg
      241 atgttttact ggagttattc attagcttgg tcaatagttc cattatttca atatgataaa
      301 cctcgacctg ttgaaaagcc acgcagcaca cttcagaaag tatataaaaa accatatgtc
      361 gttatataaa taaggaaatt gcactgcatc atggcattta ataatatgcg ttaattgttc
      421 ttttttatat tctctgaggg aaatatgttc ctggattatt ttgcactggg agtgcttatt
      481 tttgtatttc tggtgatatt ctatgggatt attattttac atgatattcc ctacctgatt
      541 gctaaaaaac gtaatcatcc tcatgccgac gccattcatg ttgctggttg ggtgagtctt
      601 tttacgctgc atgttatctg gccatttttg tggatttggg ccacgcttta ccgcccggag
      661 cggggatggg gaatgcaaaa ccatgattca tccgttatgc aactgcaaca gcgcattgcc
      721 gggctggaga aacagctcgc cgacatcaaa tcctcttctg ccgagtaata cttatggatt
      781 tactgattat tttgacctat gtggcttttg catgggcaat gtttaagatc ttcaaaattc
      841 ccgtaaataa atggaccatt cccacagcgg ccctgggtgg aatatttatt gtcagcggtc
      901 taattctgtt aatgaactat aaccatccgt atacctttaa agcgcaaaaa gcggttattt
      961 ctattcctgt tgtcccacag gtgactggtg tggtgattga agtgacggat aagaaaaata
     1021 cgctgattaa aaaaggtgaa gtgctatttc gactggaccc gacgcgttat caggcgcggg
     1081 tggatcggct gatggcggat atcgttaccg cagaacataa acagcgggcg ttgggcgcag
     1141 agttagatga gatggtggcg aatactcagc aggcaaaggc cacgcgggat aaattcgcta
     1201 aagagtacca gcgttacgca cgcggcagcc aggcgaaagt aaacccgttt tcagaacgcg
     1261 atattgatgt ggcacggcaa aattatctgg cgcaggaagc ctccgtaaag tcatcggcgg
     1321 cggaacaaaa acagatccag agccagctgg atagcctggt gttgggtgaa cattctcaaa
     1381 tcgccagcct gaaagcgcag ctcgcggaag caaaatataa ccttgagcag acgatagtgc
     1441 gtgcgccgag cgatggttat gtgacccaag tgctgattcg tccgggtacc tatgccgcgt
     1501 cgctgccgct acgtccggtg atggtgttta tacccgatca gaaacgacaa atcgtggcgc
     1561 agttccgtca gaactccttg ctgcgcctgg ctcccggcga cgatgcggaa gtggtgttta
     1621 atgctctgcc aggtaaggta ttcagcggta agctggcagc cattagtcca gctgttcccg
     1681 gcggagctta tcagtcgacc ggcaccttac agacgttaaa cacagcgccg ggttcagatg
     1741 gcgtaatcgc gaccattgaa ctggatgagc acactgattt gagcgcatta ccagacggta
     1801 tttacgccca ggtggcggtc tactctgatc atttcagcca tgtctcggtg atgcgcaaag
     1861 tactgttacg catgaccagc tgggtgcatt acctttatct cgatcattaa tcttcgcttt
     1921 ccgccgcttg ttcaggccgc agttgctgcg actgagcaag ctggtggcga atttcacgca
     1981 gcatttcatc cagcacatac tggacccgcc agggctggta ttcccgttta cggaagatag
     2041 ccaccagatc cagccaccat tcatagggtt gctgaaagca gggcaccagc gtgccgtctt
     2101 gcagatcgct ctgcacactt ttgtctggcg caaacacaat ccccaggtga tttcgtgcca
     2161 gctccagcgc cgattgcgtg ttgtcgcaaa cgtaatttcc cttcacgcga taatcccgca
     2221 cctctttact cccggcgacg ttgaagcgcc agatattcgc gtcgtcgatc atcatcgagt
     2281 cgatcaaaat acaggagtga tgttcgagtt cgtcaggacg gctaatggga tgtttctcaa
     2341 gatagcgctg gctggcataa gcggttacgg catattgggt aataaaactg gcaaccagcg
     2401 attcatcttt tggtggggca tagctgatta aaacatcaca atcatcagga aattcgacgc
     2461 cttcataaaa ggcattacgc tcaagattgc aggtttttag cgataaggtg atatcaccga
     2521 tatctttaat tttatcgatt acatgtttgg ataaataggt aattatgccg gttggggcgt
     2581 agatggtgac ccgaccacgt ttctcatact tataatctgc aataaaatta ttaagctgct
     2641 cgtttctgtc cagcatgtcg ttgatgtacg gtaacagcgc ggtaccaaaa ggtgtgagca
     2701 tcagctgccg ggtggttcgg tcaaagactt ttaaacccac ttttgattca aaatcagcaa
     2761 ggtatttgct gacgttggcc tgtgcgatgc caagtacagt cgcggcatgg ctgatatttt
     2821 cactggcagc gattaccgag ataattttta actcccggta cttaagttgt aattttgtca
     2881 tcgcaa
//
LOCUS       DAPGWD010000270         2867 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPGWD010000270 DAPGWD010000000
VERSION     DAPGWD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.273"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            2..478
                     /gene="agaB"
                     /locus_tag="RHX88_004384"
     CDS             2..478
                     /gene="agaB"
                     /locus_tag="RHX88_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV2232982.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            517..1320
                     /gene="agaC"
                     /locus_tag="RHX88_004385"
     CDS             517..1320
                     /gene="agaC"
                     /locus_tag="RHX88_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDV2232983.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1310..2101
                     /gene="agaD"
                     /locus_tag="RHX88_004386"
     CDS             1310..2101
                     /gene="agaD"
                     /locus_tag="RHX88_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDV2232984.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2102..2857
                     /locus_tag="RHX88_004387"
     CDS             2102..2857
                     /locus_tag="RHX88_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDV2232985.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          697 a          676 c          727 g          767 t
ORIGIN      
        1 aatgagcagt ccaaatattc tcttaacccg tattgataac cgtctggttc atggccaggt
       61 tggcgtgacc tggacatcca ccatcggtgc aaatctgctg gtagtcgtgg atgatgttgt
      121 cgctaacgat gatattcaac agaaattaat gggtattacc gcggaaacct acggctttgg
      181 cattcgtttc tttactatcg aaaaaaccat taacgtcatc ggcaaagctg caccgcatca
      241 gaagatcttc ctgatttgcc gtacgccaca aacggtacgt aaattggtag aaggtggcat
      301 tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg gaagggaaaa agcaaatcag
      361 cagtaaagtt tatgtcgatg accaggatct cgcggactta cgttttatta aacaacgtgg
      421 cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa gaacaaatcc ctgactaaat
      481 ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc atgaaataac cctacttcag
      541 ggattatccc tggcggcgtt agtttttgtt ctggggattg atttttggct ggaagcctta
      601 tttttattcc gcccgataat cgtttgtacc ctaactggcg ctattctcgg tgatattcag
      661 actggcttaa ttaccggtgg tctgacagag ttggctttcg ccggattaac ccctgcaggt
      721 ggtgttcagc cgcccaaccc gattatggcg ggtctgatga ccaccgtcat tgcatggtct
      781 acgggcgttg atgccaaaac agcaattggt cttggcctgc cgtttagttt gttaatgcag
      841 tacgtcattc tgttcttcta ttccgctttc tcattattta tgaccaaagc cgataaatgc
      901 gcgaaagagg cggatacggc agcgttttcc cggcttaact ggacaacgat gctcatcgtc
      961 gcttcagcgt atgcggtgat tgctttcctc tgtacttacc tggcacaggg ggcgatgcag
     1021 gcgctggtga aagcgatgcc cgcctggctg acccacggct ttgaagtggc aggcggtatt
     1081 ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt tcaaagcgca atatatccct
     1141 tacctgatcg ccggtttcct gtttgtttgc tacatccagg tcagcaacct gttgccggtt
     1201 gccgtactgg gcgcaggctt tgcggtgtat gagtttttca atgcgaaatc ccggcagcaa
     1261 gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg actacagcaa tgggatctga
     1321 aatcagtaaa aaagatatca cccgtctggg ctttcgttcg tcgctgctgc aagcgagctt
     1381 taactacgaa aggatgcagg cgggcggttt tacttgggcg atgttgccga tcctgaaaaa
     1441 gatttataag gacgacaaac cgggcttaag cgcggcgatg aaagataacc tcgaatttat
     1501 taatacccac ccgaatctgg tcggattcct gatggggtta ttaatttcga tggaagaaaa
     1561 aggagaaaac cgcgacacca ttaaaggcct caaagtggca ctgtttggcc caatcgccgg
     1621 gattggcgat gcgattttct ggtttacttt gttgccgatt atggcgggaa tttgctcatc
     1681 atttgccagc cagggaaacc tgctggggcc gattctattt ttcgccgttt acctgcttat
     1741 ctttttcctg cgcgtcggct ggacccacgt cggttattca gtcggcgtga aggcgatcga
     1801 taaagtgcga gagaactcgc agatgattgc tcgctcggca accatcctcg ggatcacggt
     1861 aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg gtgacatcgt ttgccatcga
     1921 cagtacccac agcgtcgcgc tgcaacagga tttcttcgat aaagtcttcc cgaacatttt
     1981 accgatggcc tacaccctgc tgatgtatta cttcctgcgg gtgaaaaaag cgcatccggt
     2041 gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt tccgcattcg gcattttgta
     2101 aatggaacga ggcactgctt ctggcggtgc ctctttactt aaggaatttc atcctgtgca
     2161 aacccttcag caagttgaaa actatacggc gttaagtgaa cgagccagcg aatatttatt
     2221 ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg gcgaccggag ccacgccatt
     2281 actgacgtat cattatctgg tagaaaaaat ccaccagcag caggttgata tcagccagct
     2341 caccttcgtg aagctcgacg aatgggtgga tctgccatta acgatgcctg gcacctgcga
     2401 aactttccta caacagcata tcgtgcagcc gctggggctg cgtgaagacc agctcatcag
     2461 ctttcgctcc gaagagataa atgagacaga gtgcgaacgg gtaacgaacc tgattgcgcg
     2521 caaaggcggt ctggatttat gcgttctagg attggggaaa aacggtcatc ttgggctgaa
     2581 cgaaccggga gaaagcctac aaccggcctg ccatatcagt caacttgatg ccagaacaca
     2641 gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact cgtgggatca ccttaggcct
     2701 gaaggatatt ctcaatgccc gcgaggtttt gttattggtg actggcgaag gaaagcagga
     2761 tgcgacagaa cgttttctca cggctaaagt ctccaccgct atcccggctt cgtttttatg
     2821 gctgcacagt aactttattt gtttaattaa tacctaacca gatgaaa
//
LOCUS       DAPGWD010000271         2859 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPGWD010000271 DAPGWD010000000
VERSION     DAPGWD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.274"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(72..338)
                     /locus_tag="RHX88_004388"
     CDS             complement(72..338)
                     /locus_tag="RHX88_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232986.1"
                     /translation="MLTGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(338..832)
                     /locus_tag="RHX88_004389"
     CDS             complement(338..832)
                     /locus_tag="RHX88_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232987.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLNLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            complement(888..1490)
                     /locus_tag="RHX88_004390"
     CDS             complement(888..1490)
                     /locus_tag="RHX88_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HDV2232988.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGISNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRTQAQQENISGQ"
     gene            1844..2728
                     /locus_tag="RHX88_004391"
                     /pseudo
     CDS             1844..2728
                     /locus_tag="RHX88_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          745 a          620 c          617 g          877 t
ORIGIN      
        1 tttctgtatc accctgtctc tctctgcttt ttcatggcta ctgtcatatt tatgtctgtt
       61 gcgcggtttt gttacttttt catgaccgaa cccgaaattt tgcgcgtcaa taagagacgt
      121 tgtgtaaact cagtcctttt atctggcagg ttaaccccta tcaatcttag gatgaaacca
      181 cgccatcctc tgtttgcctg gttattaatt tcataaagac gattgttttc ctctcgtagt
      241 tttgtccact ctaaacaaag tgttatatca tccagttctg tgatgtgttt cacttttcca
      301 ttgacagtta cagtgtttat tccatttcct gtgagcatca tcttatgcct tttttatctt
      361 catttgggac cgtgcttttc ttcggagtat cactattcgt taactgctgt ttatcaataa
      421 aattcctgat gttatcaagt gcttcctttg tttttagaat gcgcttgata tccatttcat
      481 cccgcccggt cattcttttt ccggacaaaa gacgctcaag aagctcctgt ttcgcatcag
      541 gagaatcttt ggcgagatcc atcaggttaa gcaaatcttc acgatcgaga ggaaagaagt
      601 attttacaaa atacttgaaa agcgaaagaa agtagtatgc tgtccatgca atacagaaac
      661 cgattctgag aaactcattg tcaactatac agaaatacag gtatacaatc aggagaatag
      721 gtaaaatcca taatactata gctaaaaaaa tatatacggg gatcaggcgt ctgtatgccc
      781 gttcatgttt attcaggatg aataaaatct tctccgcttc tgaatcacac ataacattct
      841 cttcccattt tttctgatac gtaatgtcca cctgttatca ggagtgttta ttgcccagaa
      901 atattctcct gctgtgcctg cgtcctgagc cacttttgtt ctaatgtcgc cagtctgcga
      961 ccggcactct gtgcttcaga gtctgagacc tgccctttgg ggtgaccatc caggccataa
     1021 cgtgggacgc cggcgacagc tgtccgctgc agatatacgc ggcgggatgt gatgcaggac
     1081 actgcacaga gccattcgtc catggtcagt tctgtctccg ggtgttctgc cagaaatgcc
     1141 gaaatatcat tgcttatgcc aattttcagg ggaataaatc caccaccttc agcacgaaga
     1201 tgaggaaaca gggcctttat tttttccacg cactcgtgag tatactcacg ggtccagtgg
     1261 gagggacgtg gaaaacggtg tcttttcttt ttcttcttcc tggctggcgc ttcggggata
     1321 tgtgtttcga cggcagatga atttattccg gcgggggctg attctgccgt ctgttcagta
     1381 aatacaggtg cgataatatc tgttttttcg gataaggacg gtggcgaaaa agtacgacgt
     1441 tttttcacca caatgacggg agttttcctt tcttcctgat tttttcccat aaatacctcg
     1501 gctggctggt tttactcttt aataatatca caatccataa attagtaaat ttcccatcag
     1561 gtgatttttc atggctaata agtccattct ctgaatattt atactcgagg tagttacagt
     1621 tatggcgtga cagtcgaatt gatattctac tgaaccctga cagaaacatg gttgatatat
     1681 tctctcactt taatgttgtc atgatataaa taaaaacaat tcatttgaaa ggatatcaga
     1741 agaagatatc ggggcgcaca gttcatctct gcctttgttt catggggggt tctttgtata
     1801 ttttacttac tgtttaagca tgtacataag gagtgctttc attatgccaa tgatacttgt
     1861 aaagaagaat ccccggggca aagtgattcg ggagcttagc agtgaggaag agactgcagt
     1921 aaagacggtc tgtggactga aaaggccagc aaccatggca caacataacc tggccaatga
     1981 tcttctccgg gaaatgcgtg agtatgatgc atggttacag tgtgactgta ttccgggaga
     2041 ttcgccggcc atgaattttg ctgctcttaa aaacaatacg ggtactcttt atctgtccag
     2101 ttttaatcat gagcatgcac ctgaatgccc catgtatcgt cagttatccg gaaatgaaga
     2161 ggaaacatcc ttcggagcaa gccggcatcc cgttagcacc cgtataaatt acagaaactt
     2221 tttaccacct gatgacagta attctgttat cagactgcag gcccgctctg cttaccataa
     2281 tggtgagcgc tcctccgttc gcaagaaacg tccgcgcctt ggccgtttac tcctgtcgct
     2341 tattgaagat gcagggctga ataaactgga ttccctggct aatccccgta tccggactaa
     2401 ttatcgtgag tgtcttgatg ccatacggca ggttacacta cagcaggagt atattcgggg
     2461 aagagcctta tctgaaatta tccatttccg gccaggaatg tctgaaaggt ctcaggagcg
     2521 gttaatggaa acgctggaga attcagaacg acactggcct gccagaagaa agcatatgtt
     2581 ctttcagatt tttatggcgc agcacatctg tcgtgatgct gtggaaattc actgggcgaa
     2641 cggtaatatt caggtcatca gacctgtgcg ggggatcagt attaatggtg aagcgcaggg
     2701 cggaatacgt cccccttact gggtcctaga ttctacgtca gtacttcaaa aagcataatc
     2761 aaagccttga taaatatgca ttccttcgaa attcagcttt cacccattgg gtgaaagaaa
     2821 agtgctcaaa aatatgttaa attatcagct tttatgact
//
LOCUS       DAPGWD010000272         2852 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPGWD010000272 DAPGWD010000000
VERSION     DAPGWD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.275"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1116)
                     /locus_tag="RHX88_004392"
     CDS             complement(<1..1116)
                     /locus_tag="RHX88_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDV2232989.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGSEGEGYLRLNLACSREK"
     gene            complement(1116..2696)
                     /locus_tag="RHX88_004393"
     CDS             complement(1116..2696)
                     /locus_tag="RHX88_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDV2232990.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
     gene            complement(2728..>2852)
                     /locus_tag="RHX88_004394"
     CDS             complement(2728..>2852)
                     /locus_tag="RHX88_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HDV2232991.1"
                     /translation="DFQLQRAIHEIERYLYATFKYDLILEEKLYLSIRIKVLMD"
BASE COUNT          900 a          650 c          507 g          795 t
ORIGIN      
        1 tttttctcga ctgcaggcca gattaagacg cagatagccc tctccttccg aaccataaac
       61 atgccccgcc ataataccta ctctcccttc gttaattaat cgacggtaaa atttatcaaa
      121 gggataacca agggccgagc aatcaaccca cgaaaaatag cagccctcag ctggccatac
      181 gatcaattcc ggaatatttt catttaaata ctgcaaggta aattgaatgt tagaggcaat
      241 atgtaccttt aacgacttta accattcttc acattcatta taggcaacaa tggtcgcaat
      301 tacattcagt actgatgggg agtttacgaa atcgcgatac ctcgatatcg ttctgaactt
      361 atgattaaaa ttatcgtcag ttgaaatcat atatgatccg gttagcgcag gaacattaaa
      421 tgttttcgaa atcgaattaa acaccaccac tttgtcatct tttattgtta gcgctgacgt
      481 gaaactatga ttaaaaacaa aatcactatg tatttcatct gaacataacc aaatattatg
      541 ccttgcacag catgctgcga ttctgtgaag atcgtcttcc gaccagaccg ttcctgtcgg
      601 gttatgagga ttacataata acaccatctt cgcgctttta attttaagct caaagtcttg
      661 ccagttgata acgtagcctc tcccattttc tttcatcaaa gaggttgtta gcatgtttcg
      721 accattatgc ttaatcacat caaaaaatgc gttataaaca ggcgtgaaaa tgagaatatt
      781 atctccattc aatgtcaata tttctattgc cttagatatt gaatacatca cgcttgggct
      841 ataggtgatc cattctggtt gaatctctgt tttatattgt tgatgatacc aattgactat
      901 cgaattcaga tagtcatcat gctgccaacg actgtaccct aaaatcgggt gttccagacg
      961 tttttttaat gttctgatga tagcttccgg tatcggaaga tccatatctg aaatagagaa
     1021 tggtaataca tcttttacac caaatctgtc ttcacaaaag tcccattgtg tgcaatatgt
     1081 tcctttacga gaaattgagg tattaaaaat atccatcaac atatccccat gtaattatcc
     1141 atatcccgac gtagggcgat gacttttgta ccgataatga cttgtaaggt attttgatca
     1201 agtttcacaa ccgcaattcc tccggccctt tttattgcag cctcatctat caatcccata
     1261 tccgcgatag ttaaacgcaa acgagttaca caattatcca gtgatttaat attttcttta
     1321 ccacccagag ccttaaccat ttgctccgct gcctgcaact ctgatttatc atgaactggg
     1381 atgttagcta ttgcagtttc ttcgtgagcg ttcagcgtga caggactgga ctcttcacgg
     1441 cctacagtca tcaggttaaa tttgagaatg gtccaacgga acacaaagta ataaatgaag
     1501 aaataagcca cttcaacaca taccgctaat ggccatcccg ttcttgtgcc acgtaagaca
     1561 ccaaagctaa taaattgaat cagatcgcca gtaaagccca tcttcacgcc catatatggc
     1621 agaaccatgt tagccaatcc attcataaag gcatggaaga caaacaacag cgggctgaca
     1681 aataagaaca gaaattcaat cggctcgctg acgccgccaa taattaccgt gagcacaccg
     1741 gaaatcagca atgctttgat agcccttctg ttttgagcat gagcagtgcg atacatcgcc
     1801 agcgcggctc cagccagacc atactgaatc atcagtttgc cctgctccat tttccccgcc
     1861 aaatccagcg gaatgaccgc attctctttg acgtatgcca taaacatgtt cagggtgccg
     1921 taatactctt caccgccaat cactgccgat ccgccgattg gggtaaagcg gaaaactgat
     1981 gtcaccaagt gatttaagcc aaaaggtatc gtcacgcgtt cggcaacagc atagaagaaa
     2041 taaccaacag gaccggaagt tgaaatccag tgaccaatga gcatgaaaag attgaaaaat
     2101 ggcggccaga taaaaggaat gaacagacca aatccactca tcacaatcag tgtaatgata
     2161 ggcacaaggc gtgggccact ataaaaccct aatgattcag gaatacgtaa gttaacgatc
     2221 tttttataca tgctggcgac taataaccct gccacaatcc cacccaacac gctggtattg
     2281 taggactgga tcccgagtat gatggtttgc ccatgtgtcg acatttggtc agcagctacc
     2341 aataagtcgt gctgtttaag ataaaagttc gttccaagat gcatcgccat aaagccaatt
     2401 aagccagaaa aagcgccata ggctttatca tctttctctt ttaataatcc caaaggtata
     2461 gctatcgcaa acaatacagg taaattaacg aaggcaaaca aaccaaggct aacaataaaa
     2521 tcaagtatgg ttttaattaa cggaatagcc aacactggaa tgaattttcc catatcatcg
     2581 ctggctaaac cacttcccag ccctaacatc ataccacata cacttaacag agcgatggga
     2641 tacataaatg ccttccccag gctctgaaaa aaactccagg ctttcttttg tttcatactg
     2701 gttatctcat gaaaatgtta tgtataatta gtccattaat actttgatgc gaatagaaag
     2761 atatagtttc tcttccagaa tcaaatcata tttaaaagtt gcgtacagat atctttcaat
     2821 ttcatgaatg gcacgttgta actgaaaatc tt
//
LOCUS       DAPGWD010000273         2766 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPGWD010000273 DAPGWD010000000
VERSION     DAPGWD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.276"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..623)
                     /gene="istA"
                     /locus_tag="RHX88_004395"
     CDS             complement(<1..623)
                     /gene="istA"
                     /locus_tag="RHX88_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HDV2232992.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDLGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSL"
     gene            complement(1344..1520)
                     /locus_tag="RHX88_004396"
     CDS             complement(1344..1520)
                     /locus_tag="RHX88_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232993.1"
                     /translation="MNLVDAFVKKVISGPYEEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            1912..2121
                     /locus_tag="RHX88_004397"
     CDS             1912..2121
                     /locus_tag="RHX88_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDV2232994.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            complement(2193..2666)
                     /gene="excA"
                     /locus_tag="RHX88_004398"
                     /pseudo
     CDS             complement(2193..2666)
                     /gene="excA"
                     /locus_tag="RHX88_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
BASE COUNT          731 a          596 c          633 g          806 t
ORIGIN      
        1 agcgaaggat ggaaccggtg ctgaccggtc tgatatgcgt cacgttgcag aaccacagtt
       61 ttcatattgt catacaacac ttcgcgcggc acaccaccaa agaagcggaa cgcattacga
      121 tggcaggtct ccagcgtgtc ataacgcata ttgtcagtga attcgatgta cagcattcgg
      181 ctgtatccga gaacagcaac gaacacgtga agcggtgagc gaccattacg catagtgccc
      241 cagtcaacct gcatctgtcg tccgggttca gtttcgaacc gaacggcagg ctcctgctcc
      301 tgaggaaccg agagagaacg aatgaatgcc ctgagaatgg tcattccgcc acgatatccc
      361 aggtctctga tctcgcgagc gattaccgtt gccgggattt tgtaaggatg agcatcggcg
      421 atgcgttgac gaatataatc ccggtattca tccaggagtg aagcaacagc aggtcgcggc
      481 gtatattttg gtggctcaga ttttgcctgc aaataacgtt taacggtatt gcgggagatc
      541 cccagttctc tggcaatcgc ccggctactc attccctgct tgtgcaggat tttaatttcc
      601 ataactgtct caaaagtgac cataagctct cctgaatcag gagagcagat taccccctgg
      661 atctgatttc aggcgttggg tgtggatcac tattgcaccg ttcgtgacag tttacgggaa
      721 aggctgacca cctgatattg ttgcaaaata cgaaagtcgt caaaatcgcg gtggggagct
      781 tgaggctgta agctgaaagc gtttgtgaaa gccccgggag attgttctat caatcaacca
      841 agggctctac tgtaatgcct agacaacatt atagtagccc gctgacgtcc tccacgccag
      901 agggagcttg ctacgtacag cttgtcgtta ctcagaagta ataaatgtaa aaaaaccgac
      961 tgatgaggtc ggttttctta ctgttccgtt acagagttgg tggccctgac tggaggaaat
     1021 ctcttttcag ctaacgttct tataatagtg ttctgttcat ttgataacaa ccacccttgt
     1081 aatccgcagg ttagcactgt ctggcgacac ttctgtcgtg atgacggtaa ccagaacatg
     1141 agtcttcccg gtataacagg catggtgggg cggatttgca gaaacaacag atgtaaaaaa
     1201 accgactggt gaggtcggct tttttactgt tccgctacag agttggttgc tctgggcggg
     1261 aaaagacagt ctgtatctaa cataatcaat tctatgcttt gtcccacact atgtcaaaaa
     1321 tcctgctaat caaaaaagag atctcacgtt aaaaatttgt acccttcttt aacttccagt
     1381 gcctgctcct ttgactcaaa catcagtctt gttttcccgg gaacacccca tgaaatgtat
     1441 tcaacatcta tccaccattt tccatattct tcatatggcc cggatattac ttttttgaca
     1501 aaggcgtcta ccagattcat tgttttttcc tttttgtgtt gaaaataatt aatgccagag
     1561 cacagcgaaa ataatgaatg cgctggctgt gataattgcg attttctccc atatgatatt
     1621 tttctcatgg ggggttctac ggagattatt tcttcgcatt gagataccgg tattcatgtt
     1681 ttatcttgga cttttgtttt gttgtctgtc tggcaggctg ttctgcgccc gtttgtgaca
     1741 aggttagctt atggggttaa atggtcacgg acatcatgcg gacggttccc tgtcacatgc
     1801 catctggttt tttcattcaa attgagagat attgctaagt tgagtcgctt gcgatagtgg
     1861 actcctgttt gtaagtgagt gccattgttt atttcatgaa gaggattgat tatgagcatg
     1921 cctcctgcga tagctaatac tttcttgttt gaaatgatga agagtaaatc aaaagacgtt
     1981 acacttgctg cgatctatgc attaggtgaa ggccggtgtc aggcggagaa cattactcgt
     2041 gaattacata gattgagtca aagtgatgat atggaaataa aaattgctgc gattaaagca
     2101 cttggtcgaa tttatcggta acggcataag ttatctgttg taatgaacaa aagccggctc
     2161 atttgaaccg gctgttatgg agggagctta tcttaaaata cttcggctac aggcatgcct
     2221 gcaatctgct cccactcctt acgcttttcc tgaatcaggc gaggaaagtc gacagggtaa
     2281 tcatatcctc gacttgccat tgcgtgcatg gtgtttgatg ctgtttctgg atgagcacac
     2341 catgatggaa caggaaccat tggcagattg atgtatttcg tatgaatttc cagttttttg
     2401 tcgtctgtta cagtacgagt gaaattatga atgtcaaaac cgataacatc cataatgttg
     2461 cctggataga tacggacata caacattgtg ccatttttaa ggtcaatgcc gaaataagct
     2521 ccctgggtta aagataatcc ctcatacccc ttctctggag agtagaagcc tgactctttt
     2581 attgtttgta caacttttct tattttttct ctccttgttt ttgcacgtga gtacatccat
     2641 atgcctggcg caagtaataa gaaccaacta ccgaaagtga caacataatc ttcgacctta
     2701 tttccgaaat aacgatcaga acatagcatt tcggtaaagc ctaatgcgca gaacagaaaa
     2761 aaaggt
//
LOCUS       DAPGWD010000274         2728 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPGWD010000274 DAPGWD010000000
VERSION     DAPGWD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.277"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(234..452)
                     /locus_tag="RHX88_004399"
     CDS             complement(234..452)
                     /locus_tag="RHX88_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232995.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(584..2107)
                     /locus_tag="RHX88_004400"
     CDS             complement(584..2107)
                     /locus_tag="RHX88_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV2232996.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2439..2687)
                     /locus_tag="RHX88_004401"
     CDS             complement(2439..2687)
                     /locus_tag="RHX88_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2232997.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          837 a          583 c          560 g          748 t
ORIGIN      
        1 ccttattttc atgacattga tcactttagg gtgacgattt tgtttaacaa ataactcttc
       61 gacatcaaaa ttgttaatag ttgcgatgag gattatttcg gtgatatata aaaacaaccg
      121 tgataacacc agggttgaca actatttgcg caataatgcc tttccttcaa tgatccaggc
      181 gttcactgcc agcaacccat tcagccgcat actgacagtg agaaactgaa agattattgc
      241 tgatccctgt ggttgacgag tctttctctt aatcgattaa ccagattgat cgccagccaa
      301 acaagggcat taagcaaatt tatcgtcaca agcacttctc ccagcccggt actgggtttt
      361 gcaataagga tgaccaggag ggtgatcagc ataaatgcaa tggaaaagat gatatagaga
      421 gtgaaataat ccagattatt actattgttc atgctactaa tggcaatcct gtcaaaactt
      481 tcaaacaatt atagcaaaca aaataatgaa caatagtaac aagaggcaac gagctaatta
      541 gctgtctata attacaccgc actatggcct tactgatcaa ttttcactca accacaacct
      601 tcaccttcgg tttagaaagg ataattttga ccaggtcgat gtatgtaaca ggtttataga
      661 aataataacc ctgctgaaat gtgatattat tttggttcag atagtcaagt tgttctttcg
      721 tttcgacacc ttcagcgacg atactgatcg aaagtttacg cgccagatcc aatacacaat
      781 ccagaattcg ggttgattct gggtctgcgt ttacgcggcc aacgaaactg tgatcaattt
      841 tgatataatc aatatgcagg tcatgaagat aagaaagccc tgagtagcca gtaccgaaat
      901 catccagcgc gatgacaaaa ccattttcat gcagaatgtt caaccgctga accagacttt
      961 catcaacatt caatggctca cgctcggtga cttccagaac aaggtttaaa tcgcggcggg
     1021 taaaactgtc acggaaattt aaacactcgt cgacaaacgt cggcgaaata atatgcgaag
     1081 cgctaaaatt aattccaata tgaaaacctt ccggcaattt actcgcgata gcgttcatct
     1141 gtctggcaac ctgattcatc aggctttgcg taagcggaac gattaatccg gatttttcag
     1201 caagtggaat aaatgccgcg ggtgatatat atccaccgtg aggttgtttc cagcgggcta
     1261 acacctcaac tccccgcaat gtcccttccc gaccatttac cacaggttgg taaaaaggga
     1321 cgatctcccc attatttatt gcccgtcgca ggatctcttc aggcgttgcg cttttattga
     1381 aatatctatc aagcaggaag gcagcggcgc aggcgattaa cagaataaat attaaaattc
     1441 caaagccttt atcgataagt ctgtagaagc taaagagtgg cggcgggtta aagataatag
     1501 tgaaatgata ttttgtggag tgaaccttct cggtataggg ataatgtcca ttaagcgtca
     1561 tcacatcacc cgttggtcca ataatgtcat ccgccacgcg taatacatac cttacccctt
     1621 tcaagggtac attaagcgcc ccgcgaatat gttgatcact tatcgtaacc aaaatgcgcg
     1681 tatctgcaaa ttggttctga tagagcagga taggtaatct attaacggtg tcgattgctg
     1741 gagccaacag taaattactg tccgggaaaa caggaatacg agacaacagg acccgattcc
     1801 caggcaggga tgtacaccag actattccct gtttgagaat gatgattgtg cgcaggtgag
     1861 gtttcagagc tgcttcagtg ccaagctgat attgctcctc gaggttgcat tccttcccgg
     1921 caatgttaac cgctgtttga gtcgcttgtg acgcttcttc aagtatatga ttgatattgt
     1981 tggcggcata tctcgctccc gccaggtact ctgcttttgc ggaataccag agctggatgt
     2041 tcaaaatagc gacaccggta ataaacaagc atatcgcaac gaggatgggt atgagtgtat
     2101 tgcgcatttg cttgtcccgg ccaaagggtt taatacatga aaatgccatt cattcgctcg
     2161 cgctggcatc tacagagagc atggtagaga gcataagaaa tatccacgcc gaagataaaa
     2221 ttttctccat cttgatctaa cccattaatt attgagcata atgtaggcag tacaaaataa
     2281 gttaggcggg atatcaggcg tcaagaatgg agagcgagaa ctctccattc ttgacacctg
     2341 atattgcgga cataataaga aagcataacg cctgaaatgc tcactttgca tcagcatggt
     2401 gatacagctg atgtttattc taaaacctta ctcaagttct aagagagcac ggattccctg
     2461 tcattaataa tgatatccgt tagtgtagtc ttatgcattt caatgtactg caatatttgc
     2521 agatagagat cagaaagaat cacactggtt tcatctttta aacgcaagcg taaagcctga
     2581 tcaataatat gactatcaac atacccatgc gtcatgaaca cctccctctc gagattttta
     2641 aacaaagcac attgaaaaat aaatctctct gggattgaat tattcatgtc agcctcgtag
     2701 aaatattaac ccaataaaat tatgctgt
//
LOCUS       DAPGWD010000275         2727 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPGWD010000275 DAPGWD010000000
VERSION     DAPGWD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.278"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1386
                     /locus_tag="RHX88_004402"
     CDS             <1..1386
                     /locus_tag="RHX88_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV2232998.1"
                     /translation="ATECIAADVILDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVA
                     RGGNQIRMIGGEYGALWRNDGAKTYLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTK
                     LSASLNGNALTATKLQTPRRVSGVEFDGSKDITLTAAHVAAFARRATDTYADADGGVP
                     WNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDW
                     AEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPD
                     MRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKNTSSFDYGTKSTNNTGA
                     HTHSISGTANSAGAHQHKSSGAFGGTNTSIFPNGYTAISNLSAGIMSTTSGSGQTRNA
                     GKTSSDGAHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVK
                     NIAFNYIVRLA"
     gene            1386..1961
                     /locus_tag="RHX88_004403"
     CDS             1386..1961
                     /locus_tag="RHX88_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV2232999.1"
                     /translation="MAFRMSEQPQTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(2059..2649)
                     /locus_tag="RHX88_004404"
     CDS             complement(2059..2649)
                     /locus_tag="RHX88_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV2233000.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          720 a          613 c          674 g          720 t
ORIGIN      
        1 gcgactgaat gtatcgcggc ggacgttatc cttgatttta agagtgggcg tggtttttat
       61 gagtctcatt cgttaatcgt taacgataac ttgtcgtgca aaaaactttt tgccacagac
      121 gaaattgtag cgcgtggtgg taatcagatt cgaatgatag gtggggagta tggggcatta
      181 tggcgtaatg atggcgctaa aacttacctg ctgcttacca atcaaggtga tgtttatggt
      241 ggctggaata cattaagacc gtttgctatt gataacgcaa ccggtgaact ggttattgga
      301 accaaactgt ccgcaagtct gaacggtaat gcattaacag caacaaagct gcaaacgcca
      361 agacgggttt ctggtgttga gtttgatggt tccaaagata ttactttaac cgccgcgcat
      421 gtggctgctt ttgccagaag ggcaacggat acatatgccg atgcggatgg tggcgttccc
      481 tggaatgccg aatcaggcgc ttataatgtc acccgctctg gcgacagcta tattctggtt
      541 aacttctata ccggagtcgg aagttgccgg accttgcaga tgaaggcgca ttacagaaat
      601 ggtggtctgt tctaccgttc ttcaagagac ggttatggtt ttgaggaaga ctgggcagaa
      661 gtttatacct cgaaaaatct tccaccagaa agctacccag tcggcgcacc aatcccgtgg
      721 ccatcagata ccgttccgtc tggttatgcc ctgatgcagg ggcagacttt tgacaaatct
      781 gcctacccga aacttgcagc cgcttatccg tcaggcgtga tccctgatat gcgtggctgg
      841 acgattaagg gcaaacccgc cagtggtcgt gccgtattgt ctcaggaaca ggacggcatt
      901 aaatcgcaca cccacagcgc cagcgcatcc agtacggatt tggggacgaa aaacacatcg
      961 tcgtttgatt acggaaccaa atccacgaat aacaccgggg cgcataccca tagtattagc
     1021 gggactgcaa atagtgccgg tgcgcaccaa cacaagagtt ccggtgcatt tggtggcacg
     1081 aacacgagca ttttccctaa tggttatacc gcgatttcaa atctaagcgc ggggattatg
     1141 agcacaacaa gcggtagtgg ccagactcgt aatgcaggga agacatcatc agatggtgct
     1201 catacccact cgctgtccgg cactgctgca agcgcaggcg cacatgcaca tactgtcggt
     1261 attggtgctc atacgcactc cgttgcgatt ggttcacatg gacacaccat caccgttaac
     1321 gctgctggta acgcggaaaa caccgtcaaa aacatcgcat ttaactatat tgtgaggctt
     1381 gcataatggc attcagaatg agtgaacaac cacagaccat aaaaatttat aatctgctgg
     1441 ccggaactaa tgaatttatt ggtgaaggtg acgcatatat tccgcctcat acaggtctgc
     1501 cagcaaacag taccgatatt gcaccgccag atattccggc aggcttcgtg gctgttttca
     1561 acagtgatga ggcatcgtgg catctcgttg aagatcatcg gggaaaaacc gtctatgacg
     1621 tggcttccgg cgacgcgtta tttatttctg aactcggtcc gttaccggaa aatgttacct
     1681 ggttatcgcc ggaaggggag tttcagaagt ggaacggcac agcctgggtg aaggatacgg
     1741 aagcagaaaa actgttccgg atccgggagg cggaagaaac aaaaaacaac ctgatgcagg
     1801 tagccagtga gcatattgcg ccgcttcagg atgctgcaga tctggaaatt gcaacggagg
     1861 aagaaatctc gttgctggaa gcatggaaaa agtatcgggt attgctgaac cgtgttgata
     1921 cgtcaactgc acaggatatt gaatggccag cactgccgta gggtaaaaca tataaattct
     1981 ataattagat gtatctttcc atttacggca aggaaggggg cttggaacac gtaaagcatc
     2041 tcacaccgag attatttttt atatgtcagg tgtctgaagt tttgctttgg ctcttaaaat
     2101 ggtttgccgc gaggttttga attcccgggc aatggcactt atacttacac ctgacttaat
     2161 tcgttcgaat accacctgtt tctgttcttc atttaacaca ggtggtcgac caaaacgttt
     2221 ccctgcgccg cgggctctta ctatcccgga atgagtgcgt tcaagtaaaa ggtctcgttc
     2281 aaattcagcg actgctgaaa ttacttgcat catcattttt cctgttggac tggtcaggtc
     2341 aatgccaccc aatgctaagc aatgcactct gatacctgtt tcggtcagtt gttccactgt
     2401 tttcctgata tccattgcat tacaaccaag gcgatccagt tttgtcacaa tcaattgatc
     2461 accacatttc aggcgagcaa gcaaccggtt aaaaccagga cgctcactgg ttgctgctga
     2521 gccgctaatg tgttcttcga ttatttgctg aggtttgatt ttaaaacctg cactttcgat
     2581 ttcccggcgt tgattttcgg tggtctgatc cagcgttgat atccgacagt aagcaaaaat
     2641 tcgagacata gtgagactct attcgaaatt ggtgttcata tcataatgca tctcagaaaa
     2701 taattatgat tatttttgtg catattt
//
LOCUS       DAPGWD010000276         2721 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPGWD010000276 DAPGWD010000000
VERSION     DAPGWD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.279"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            193..1359
                     /gene="nhaA"
                     /locus_tag="RHX88_004405"
     CDS             193..1359
                     /gene="nhaA"
                     /locus_tag="RHX88_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDV2233001.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1425..2324
                     /gene="nhaR"
                     /locus_tag="RHX88_004406"
     CDS             1425..2324
                     /gene="nhaR"
                     /locus_tag="RHX88_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDV2233002.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2363..>2721)
                     /locus_tag="RHX88_004407"
     CDS             complement(2363..>2721)
                     /locus_tag="RHX88_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDV2233003.1"
                     /translation="NYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILK
                     NELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNV
                     SSGQIMSRVKIVVMYN"
BASE COUNT          633 a          603 c          695 g          790 t
ORIGIN      
        1 ctatgattat ctgggcttag ggtcactcgt gagcgcttac agccgtcaaa aactcatctc
       61 accgctgatg gcgcaaattc ttcaatggat cgtaaaaaac gagttattcc tacactataa
      121 tctgatttta acgatgattt gtgcggggta aaatagtaaa aacgatctat tcacctgaaa
      181 gagaaataaa aagtgaaaca tctgcatcga ttctttagca gtgatgcctc gggaggcatt
      241 attcttatca ttgccgctat cctggcgatg attatggcca atagcggcgc aaccagtgga
      301 tggtatcacg actttctgga gacgccggtt cagctccggg ttggttcact cgaaatcaac
      361 aaaaacatgc tgttatggat aaatgacgcg ctgatggcgg tatttttcct gttagtcggt
      421 ctggaagtta aacgtgaact gatgcaagga tcgctagcca gcttacgcca ggccgcattt
      481 ccagttatcg ccgctattgg tgggatgatt gtgccggcat tactctatct ggcctttaac
      541 tatgccgatc cgattacccg cgaagggtgg gcgatcccgg cggctactga cattgccttt
      601 gcacttggtg tgctggcgct gttgggaagt cgtgttccgt tagcgctgaa gatctttttg
      661 atggctctgg ctattatcga cgatcttggg gccatcatta tcatcgcatt gttctacact
      721 aatgacttat cgatggcctc tcttggcgtc gcggctgtag caattgcggt actcgcggta
      781 ttgaatctgt gtggtgtacg ccgcacgggc gtctatattc tggttggcgt agtgttgtgg
      841 actgcggtgt tgaaatcggg ggttcacgca accctggcgg gggtaattgt cggcttcttt
      901 attcctttga aagagaagca tgggcgttct ccagcgaagc gactggagca tgtgttacac
      961 ccgtgggtgg cgtatctgat tttgccgctg tttgcatttg ctaatgctgg cgtttcactg
     1021 caaggtgtca cgctggatgg cttgacctcc attctgccat tggggatcat cgctggcttg
     1081 ctgattggca aaccgctggg gattagtctg ttctgctggt tggcgctgcg tttgaaactg
     1141 gcgcatctgc ctgagggaac gacttatcag caaattatgg cggtggggat cctgtgcggt
     1201 atcggtttta ctatgtctat ctttattgcc agcctggcct ttggtagcgt agatccagaa
     1261 ctgattaact gggcgaaact cggtatcctg gtcggttcta tctcttcggc ggtaattgga
     1321 tacagctggt tacgcgttcg tttgcgtcca tcagtttgac aggacggttt accggggagc
     1381 cataaacggc tcccttttca ttgttatcag ggagagaaat gagcatgtct catatcaatt
     1441 acaaccactt gtattacttc tggcatgtct ataaagaagg ttccgtggtt ggcgcagcgg
     1501 aggcgcttta tttaactcca caaaccatta ccggacagat tcgagcgctg gaagagcgcc
     1561 tgcaaggcaa attatttaaa cgcaagggac gtggtctcga acccagcgag ctgggagaac
     1621 tggtctatcg ctatgccgat aaaatgttca ccttaagcca ggaaatgctg gatattgtga
     1681 actatcgcaa agaatccaat ttattgtttg acgttggtgt ggctgatgca ctttccaaac
     1741 gcctggtcag tagcgtactt aacgccgcag tggtagaagg cgagcccatt catcttcgct
     1801 gcttcgaatc cacccacgaa atgctgctgg agcaattaag tcagcataaa ctggatatga
     1861 tcatttctga ctgtccgata gactctacgc agcaggaagg cctgttctcc gtgagaattg
     1921 gcgaatgtgg cgtgagtttc tggtgtacaa atccaccacc agaaaaaccg ttcccggctt
     1981 gtctggaaga acggcgactt ttgattcctg ggcgacgttc aatgttaggg cgcaaattgc
     2041 ttaactggtt taactcccag ggattaaacg tagaaatcct cggcgagttt gatgatgccg
     2101 cgttgatgaa agcttttggt gcgatgcaca atgcaatctt cgttgcccca acgctttatg
     2161 catatgactt ttatgccgat aaaactgtcg tagaaattgg tcgcgtcgag aatgtgatgg
     2221 aagagtacca tgctattttt gctgagcgga tgattcagca cccggcggta cagcgaatct
     2281 gcaatacgga ttattctgcg ctttttagtc cagcggcgcg ttaatcggca cctcccccca
     2341 aagttaaggt gggggagata gattagttgt acataaccac gattttgact cggctcatta
     2401 tttgcccgct tgagacatta tttccatatg tacgcgggcg aataaataga ggaatctgat
     2461 tacttccttc atggggatgc tgaaaagagt agtaattgct tttaccatac tggatttcat
     2521 tcgtaccatc attggagagt tgaaagccta tttgacccgc accgtcctca ctgttttcta
     2581 actcattttt taatatgctg gttcctgact cctgtatcgt cactggttca aagtaatatt
     2641 caacatcact gggttcaaaa actaattcac cttgctcgcc ctggatgcag tcaaatctta
     2701 tatgatgaaa ctgataattt t
//
LOCUS       DAPGWD010000277         2715 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.280, whole genome shotgun sequence.
ACCESSION   DAPGWD010000277 DAPGWD010000000
VERSION     DAPGWD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.280"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(301..1707)
                     /gene="gndA"
                     /locus_tag="RHX88_004408"
     CDS             complement(301..1707)
                     /gene="gndA"
                     /locus_tag="RHX88_004408"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDV2233004.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(1859..2356)
                     /locus_tag="RHX88_004409"
     CDS             complement(1859..2356)
                     /locus_tag="RHX88_004409"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HDV2233005.1"
                     /translation="MKVSFVSQDDFENLIQEAFASVRLRSHLLLHESPNDAVQRIMIG
                     LVKGTYIPPHFHEFQHQWEHFHVFQGEVELMLFDSNGCLNKKVILGGQSKNIIAQISP
                     LTPHTLVCRSPTAVIMEIKEGPFDEKCAKVIPSWSYSEDYSILSRDRIIAMMGQLSIG
                     DRFSL"
     gene            complement(2375..>2715)
                     /locus_tag="RHX88_004410"
     CDS             complement(2375..>2715)
                     /locus_tag="RHX88_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase WbuB"
                     /protein_id="HDV2233006.1"
                     /translation="KSVLKIVDVGLFSLAKNHTVHNFPGKLLGYMANKLPILGSVNAS
                     NDVMEIINGAKAGFVFVNGNDEALLNAAINLADDTQLRKNLGCNAYSLLQEKFSVEMA
                     AEKILSSLFS"
BASE COUNT          780 a          610 c          570 g          755 t
ORIGIN      
        1 gaagcccgtt tgacaagcct acatagccag taccggaaat ggtgattttc atttcatgct
       61 ctcagaatta acttaactgt gaatcatgat gtttttagca tcctgataag agctaaaagt
      121 tttaacgcta cgttgttcga tggtcaacgc aacgaaaaat aattcagatt ttctttataa
      181 gaatagctga tatttattaa gttaatatta agcagtgaaa tttagtctat aagataattg
      241 gcaaaaaaaa gcccggtgca atatacgccg ggcctcaatt ttattgttgg ttaaatcaga
      301 ttaatccagc cattcagtat ggaacacacc ttctttatca atgcgcttat aagtatgcgc
      361 accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg cacggtagct
      421 atcgtaatag gcaaccgcag cagcgaaggt cggaaccggg ataccgttct gtactgcata
      481 agcaacgaca tcacgcagcg cctgctggta gtcatcggca atttgcttga agtacggagc
      541 cagcagcagg ttagcgatct gcggattttc ggcataagca tcggtgattt tctgcaagaa
      601 ctgcgcacga atgatgcaac cagcacggaa aatcttcgcg atttcgccgt agttcagatc
      661 ccagttgtac tcttcagacg ctgcgcgcag ctgagagaaa ccctgagcgt aagaaacgat
      721 tttgcccaga tacagagcac gacgaacttt ttcgatgaac tcacctttgt cgccagctgg
      781 ctgcgcttgc gggccagaga gaactttaga tgcggcaaca cgctgctctt tcagagaaga
      841 gatataacgt gcaaacacag actcggtaat cagcgacagc ggttctccga gatcaagcgc
      901 gctctggctg gtccatttac cggtaccttt gttagccgct tcatccagga tcacatcaac
      961 caggtagtta ccgtcttcat cttttttggt gaagatgtct ttggtgatgt cgatcaggta
     1021 gctgctcagt tcaccgttat tccactcggt aaaggtctgc gccagttctt cgttggtgag
     1081 gttcagacca cctttaagca gagaataggc ttcagcaatc agctgcatat cgccgtattc
     1141 aataccgttg tgaaccatct tcacatagtg acctgcgcca tcggcaccaa tataggtaac
     1201 gcatggctca ccgtcttcag ccactgcggc gattttggtc aggatcggcg caacaagttc
     1261 ataggcttct ttctgcccac caggcataat ggaaggacct tttagtgcgc cctcctcacc
     1321 accggaaaca ccggtaccga tgaagttaaa gccttctgcg gaaagctcac ggttacgacg
     1381 aatggtgtcc tggaagaagg tgttaccacc atcgatgatg atgtcgcctt tatcgagata
     1441 tggtttgagg gaatcaatag cagcatccgt gcctgcacct gctttcacca ttaacaggat
     1501 gcgacgaggc gtttccagag attcaacaaa ctctttcacc gtatagtaag gaaccagttt
     1561 cttgcccgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt tgaaaataga
     1621 gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca ctgccatacc
     1681 gactacgcca atctgttgct ttgacattgt ttactcctgt caggggtggt caccgcgacg
     1741 tatgcacggc ttgaatgtag tgtagatgtt aactcagtat tcaaagctat aataatagtg
     1801 gataaatata atatagaagg tatgaaaaag aagattactt cgctaattgc tgtgaagttt
     1861 agagactaaa tctatctcca attgataatt ggcccatcat tgcgatgatc ctatctctgg
     1921 ataaaatgga ataatcttca ctatatgacc atgaggggat gactttagcg catttttcat
     1981 caaaaggacc ttctttaatc tccatgataa ctgctgtagg agatctacaa actagtgtat
     2041 gtggagtgag tggtgaaatt tgtgcaataa tattcttact ctgcccacca agaataacct
     2101 ttttatttaa acagccgtta ctatcaaata gcattaattc aacctcacct tgaaacacat
     2161 ggaaatgttc ccattgatgc tgaaattcat gaaaatgagg cgggatatac gttcctttga
     2221 caagtcctat cattattctc tggacagcat cattagggct ttcatggagg agtaagtgag
     2281 atctcaagcg aacggatgca aatgcttcct gaattaaatt ctcgaaatca tcctgactta
     2341 caaaagacac tttcataaaa ccaccatatt actgttagga aaataagctg cttaatatct
     2401 tttcagcagc catttccaca gagaattttt cttgtaataa agagtaagca ttacatccca
     2461 ggttttttct tagctgagta tcatccgcaa gatttattgc agcattaagt aaagcctcgt
     2521 catttccgtt aacaaaaaca aacccggcct ttgccccatt gataatctcc ataacatcat
     2581 tgcttgcatt aacactacct aatataggga gtttatttgc catataccct aacaatttac
     2641 cagggaagtt atgaacagta tgatttttag caaggctaaa caaaccaaca tcaacaatct
     2701 ttaatacaga cttaa
//
LOCUS       DAPGWD010000278         2710 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPGWD010000278 DAPGWD010000000
VERSION     DAPGWD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.281"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1994)
                     /locus_tag="RHX88_004411"
     CDS             complement(<1..1994)
                     /locus_tag="RHX88_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDV2233007.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHAADAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASVSATAAGKSAESAASSA
                     STATTKAGEAAEQASAAARSASAAMTSETNAKASETSAESSKMAAASSASSAASSASS
                     ASASKDEASRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTT"
     gene            complement(2059..2658)
                     /locus_tag="RHX88_004412"
     CDS             complement(2059..2658)
                     /locus_tag="RHX88_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDV2233008.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVSG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVIDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          517 a          802 c          708 g          683 t
ORIGIN      
        1 taggtcgtat cattaacaac gagatcagca ccaggtgcgg atattgacag gccatcttcg
       61 ataaacgcaa aaacagggaa agcagcgcca tcaacataga acacagagcg caaatcatcg
      121 cccttattac tcatcattat tgagtggatg gctcgttcat tgttttgata ttgccagaac
      181 attccataag cataacgccc cctgtcagtc cagccaccag gcataacaaa tccgttaaac
      241 tcgcagttat tcatcggatc gcctgcggtt cgcgttgccg tggtgataat gacccttgat
      301 gccagttcgc ttactgagcc agcagaacgc ataacaacaa cagggtaata ttttccagat
      361 gttgcacctg caggagcgtt aacccgcaca taacgcatac cacgcttatc agcaaagtct
      421 gttttactga ccgcgttaat gttgttcagg aagcgtccct tatcgggtat atcagcgccg
      481 ttctggtctt tctgcagacg tttctcagca ttgtcattgg ctgcttttac tgcctttggc
      541 gttgccgcca gcgtttcaga cgtgctgttg gtcgcactgc tgagctgtac tatccccttt
      601 ttcgtcgtgc tcgcatcctc aagcgccacg gcggatgcaa tatcctctgc ccgttttgct
      661 gctgtctcgg cgcgcgttgc cgcggattcc gccgtacttt tgctctgagc tgccgccgtc
      721 gcactaccag ctgcctctgt cgccttcgtg gatgccgtcg tggcgctgcc tttcgctgct
      781 gatgcctgtc tgctcgcctc atcttttgaa gcagacgcag atgatgccga tgacgccgcc
      841 gaactggcgg acgatgcagc tgccattttt gaggattctg cactggtttc cgaggctttc
      901 gcgttcgttt cggatgtcat cgctgcggat gcagacctcg ctgctgcgct ggcctgttca
      961 gcggcttcgc cagccttcgt tgtggctgtt gaagcggatg atgcggcgct ttctgccgat
     1021 tttccggctg cggtggcact gactgaggcc tgcccggcac ttgttgacgc ggcactggca
     1081 gacgacgcag ccgctgtttt tgagcctgcc gcagctgagg cgctctgtcc cgctgccgtc
     1141 tcagaagacc tggcgttcgt ctcggacgtt tttgccgcct tcgcggaatt tcctgccgct
     1201 gttgccgagg aggctgcact actggcgctc gaggctgcgc tcgtttctga tgattttgcc
     1261 gcctcttttg aagccgacgc atcccgtgct gaagtggctg cttctgacgc tttcgtggtc
     1321 gcggtggatg cagaagtggc ggctgattgt tctgacgctg ccgcattcgt ttctgacgtt
     1381 ttcgccgcac cggcactggt ggccgccgcg ctttttgagg actctgcagc ggcagcactt
     1441 tttgaggctt cagtggcctt tgttgatgcc gttcctgcgc tggaagacgc tgactgagcc
     1501 gacgacgcgg cctgtccggc tgacgtgctg gctgcgcgtg ctgagtccgc agcatcagcc
     1561 gcatgggttg ccgcctcacg ggctgatgtg ctggcatcac tggctgactt cttcgcggct
     1621 gccgtgttct gtgccaccgc ggacgcgtta cgcgccacct cttccaccat cagttcaaaa
     1681 cggcgcagtg cctccggacg ggcatcatcc tccgtcatgg caccgagaaa atcattcagc
     1741 gtaccgggtt gagaatcttc atacacggtg atggtcccgg catgtgacgg cgggaatcct
     1801 tccaccaaca gaataacgct gtactgaccg tactcaacgt ccatgctgta acgcccggct
     1861 tcatccggat tttctgaggc cagcgtgttc accaccacag tggtactgtt acgttttgct
     1921 ttcagctgga ttgtgcagtt ctgtaccggt tttcctgtgc cgtctttcag tacacctgaa
     1981 atctttactg ccatattcac cccacaaaaa agcccgtctg aaccggcggg ctgtcataac
     2041 actgtgttac ctggctaatc agaatttata accgacaccc acgatgaaac cgtcagtgcg
     2101 ccagtcacca ctgccggagc cttcataagc aatatcaatg gccacggatt cggtcgggtt
     2161 aaactgcacg ccagctcccc acgccagaga cgtgttgctg tggcgaccgt catcacttcc
     2221 ggtcagcacg tcgtgcgttt tccccttgtt gtcaattacg cggagataat ccccggagaa
     2281 agtcgaaaca cggctgtaag ccacacccgc catcgcatac gcgctgaacc attcattcac
     2341 gcgcacagac ggccccgcca ttacgctgaa ccagcggtta cgcacggaat cttcatgcca
     2401 gcgggtatcg ctgtaacggg taagctggcg attcttgtct cctgcatagc tgaatgacgt
     2461 caccattccc agcgtgtccg taaactcata acgatatttc acgttaatcc cgttcagatc
     2521 atcgctgccg gaaacgttcg tcgaggcatg aagatagccc gcgctcagcg tggactgatg
     2581 ttcagacgcc catgcaggcg caccggatac ggccagacaa atggctgcgg acaaaatggc
     2641 ggcataaagt ttacgcataa ttacctctcg cttttctgca ataaaaaagg cgtcatttct
     2701 gacgcccgtt
//
LOCUS       DAPGWD010000279         2651 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPGWD010000279 DAPGWD010000000
VERSION     DAPGWD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.282"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(50..325)
                     /locus_tag="RHX88_004413"
     CDS             complement(50..325)
                     /locus_tag="RHX88_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDV2233009.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(319..963)
                     /locus_tag="RHX88_004414"
     CDS             complement(319..963)
                     /locus_tag="RHX88_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2233010.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAARAEIEHLMNEVFGPW"
     gene            1192..2163
                     /locus_tag="RHX88_004415"
     CDS             1192..2163
                     /locus_tag="RHX88_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein parM"
                     /protein_id="HDV2233011.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLAPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVMD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSD
                     TSFLVADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYGSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            2168..2560
                     /locus_tag="RHX88_004416"
     CDS             2168..2560
                     /locus_tag="RHX88_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HDV2233012.1"
                     /translation="MDDERKRKKFTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRAVLTELGALQ
                     SAEKMPPSATDSVQEAMNDVRLKMQKLF"
     gene            complement(2565..>2651)
                     /locus_tag="RHX88_004417"
     CDS             complement(2565..>2651)
                     /locus_tag="RHX88_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HDV2233013.1"
                     /translation="TAPEWQMKREMLSPSYTTQWTEIPIASF"
BASE COUNT          684 a          510 c          604 g          853 t
ORIGIN      
        1 acagtgtgaa ttcacaagtg tgatttcata agtaataact tcttgattat tacgttagtg
       61 tttttaagta ttgctcaagg gctactctga cgatggcact tacatttttt ggatctttac
      121 cattgcgttt attcctcaat gccagatctt ctattgtgac aagcatgctt tcaccaagag
      181 agattgtcgt ccgacattgt ttttctggtt cgggtttttc cggcgcgccg tatggtttat
      241 ccgcaaggcg ctgagccaga gcctctgctt gttctaccgt aactgcagct ctgtttaatg
      301 cttgctggct gggtttcttc accatgggcc aaatacctca ttcattaaat gttcaatttc
      361 tgctctggct gctgtattgt tcgtttctac tacaccggtg ccgttgctca tgcaatcgcg
      421 atagactttt ctgaaacaaa taactgaatc caggacttga attgttggga attcttcaag
      481 atattcaagg aattcttttc tctcatttcc cctcaaaacc ggattggttg ttgccatact
      541 ttgtaaacaa taaaccttaa gttccgggtt aaggttacgc attgcgtcta tctgttgttc
      601 tagttctgta agagtatcca gatctggttg agaacactgt agtggcgcaa ttatttgatg
      661 tgcgacaact ccactcgtta tgaattcctt tgagtttctt cctgcgacat ccactattac
      721 atcatcgaac ttttcattca gagcctgtag tgtttgtgtc aggttatcaa atttctcaac
      781 aagggttatt gctggtaaca gtcctgcagc ttctcgttct gcatgatgtt ttgcggctgt
      841 tcgttgtaga tctccgttta acaggcagac ttcccttcct ttatttgcga gagcaatagc
      901 caggtttgat gcaatggtag ttttccctga accacctttg ttcccaccaa ctacagtaat
      961 cattgcaacc tcataaatgt gatgtgtgaa gtatgatgat attttgacac ggtaacctga
     1021 tgtgtcaata tgcattcata tttatgaagt atgaaatatg aagtgtgtca tatgatgtaa
     1081 taaatattac ttgtgttttg tgatttcata actatgatag tgctcaaatt gagtattacc
     1141 ttgcaaccag aaacagttat ttactcagat tgagtattta aggggctata gatgaacgta
     1201 tactgcgatg atggttcaac aacaatcaag ttggcatgga atgacaatgg gaaaatctgt
     1261 aagtcgttgt cgcaaaactc atttcgtcat ggatggaagg ttgacgggtt agggattcgc
     1321 cagacattta actatgaact ggatggtaaa aaatacacgt atgatgaagt tagcaaccaa
     1381 tctattctta cgactcatat tgagtatcaa tatacggatg ttaacctgtt agctgtgcat
     1441 cacgctttac tgaatagtgg attagcgcct cagcctgtat cattgactgt gactttacct
     1501 atcagtgagt tctacacgaa agagtgtcaa aaaaatgaac tcaacattca gcgtaaaatt
     1561 gagaatttaa tgaggccgat tcgtcttaat aaaggcgatg ttttcaccat tgaacacgtt
     1621 gatgtgatgc ctgaatcatt gccagctgtg ttttctcgtc tggtcatgga taaagttggt
     1681 cagttcgaaa aatcgctggt agttgatatt ggcggcacta cgcttgatgt tggtgttatc
     1741 gttggtcaat ttgactctgt aagtgccatt catggtaatt ctggcattgg tgtgtcttca
     1801 gttacgaaag ctgcaatgag tgcattgcgc atggcatcca gtgataccag tttcctcgtt
     1861 gcagatgaat tgattaaacg tcgtaatgat ccggattttg ttcggcaggt tataaatgac
     1921 gaaacaaaaa ctgatctggt ccttaatacc attgaggggg ctattgccag tctgggggag
     1981 caagtagtca atgagctcgg tgattttcat catgtgaatc gtgtttatgt tgtcggtggt
     2041 ggggccccgt tgatctatgg ttcgataaaa acggcatggc accatcttgg gcaaaaagta
     2101 gtgatgatgg agtcgccaca gacagcactg gttgaagcta tcgcggcttt caaagaggag
     2161 taatattgtg gacgatgaac ggaaaaggaa aaaattcaca ctttatctgc atccggaaaa
     2221 agctgcagac tttcaaactt tggaagcgat agagtccgtt ccgcgttctg agcgtggtga
     2281 gctgttccgg aatgcattta tttctggaat ggcgttacac caacttgatc ctcgcctgcc
     2341 tgtattgctg actgccattt tgagcgagga gttttcagca gatcaggttg taaccttact
     2401 cagtcagacg actggatgga agccttctca ggccgatatc agagctgtgt tgactgaact
     2461 cggtgctttg caatctgctg aaaaaatgcc tccttctgct actgattcgg tgcaggaggc
     2521 aatgaatgat gtgcgcctta aaatgcaaaa attattctga tatatcagaa tgaagcaatg
     2581 gggatctcag tccattgtgt tgtatacgaa ggcgagagca tttcccgttt catttgccat
     2641 tcaggggcgg t
//
LOCUS       DAPGWD010000280         2635 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPGWD010000280 DAPGWD010000000
VERSION     DAPGWD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.283"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(57..1247)
                     /gene="aspC"
                     /locus_tag="RHX88_004418"
     CDS             complement(57..1247)
                     /gene="aspC"
                     /locus_tag="RHX88_004418"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDV2233014.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1432..2520)
                     /gene="ompF"
                     /locus_tag="RHX88_004419"
     CDS             complement(1432..2520)
                     /gene="ompF"
                     /locus_tag="RHX88_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDV2233015.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          653 a          650 c          634 g          698 t
ORIGIN      
        1 ttgcgtttgt catcagtctc agcccgcttt tcagcgggct tcattgtttt taatgcttac
       61 agcactgcca caatcgcttc gcacagcgga gccatgttat ctggtgtcat cccggccaca
      121 tttacgcgac cagaagcaac cgcatatacg ccaaactctt cgcgcagacg cagcacttgt
      181 tcttttgtca ggccactgaa ggagaacatg ccgttctgtt tgatgataaa gctgaagtcg
      241 cggtttgcgc ctttttcctg cagcgtattg acgaacaact gacgcatacg ctgaatacgc
      301 tggcgcatat cagtcagctc ttgttcccaa atcgcacgta acgcatcgtt gctcaggatg
      361 gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt agttagcgcg aatcgccgct
      421 ttcatttggc tgaatgcgcg atcaacggtt tcactgtcgg cagcaaccag agtacaagcg
      481 ccaacacgct cgttgtacag gccaaagttt ttagagtagg aactggcaac aatcagctct
      541 ttatgcatag ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc acgggcaaaa
      601 ccctggtaag cgaagtcaaa cagcggtaac cagcctttct caactgagag ttgtgccagt
      661 gtctgccatt gttccagcgt agggtcgata ccggttgggt tatggcagca gccatggaac
      721 agcactacgt cgccagcctg agcttcattc aggctgttaa tcagtgcatc gaagtcaaga
      781 gtgtgatttt ccgcatcata ataagcgtat tcacgaactt ccagacctgc agagttaaag
      841 acgctcttat ggttcggcca gcttgggttg ctcacccaca ctcgcttaac gctggtattt
      901 tttgccagga aatcggcagc tacgcgtagt gcgccagtgc cccccggagt ctgtgccgtg
      961 cgagcacgtt tgtcattgat cagggcgcta cctttaccaa acagcagttc ctgagtgcag
     1021 cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt tggtggtttc attttcgagc
     1081 agatactgtt cagccttttt cacgctggtc agtaccgggg ttttgcccgt ctcatcttta
     1141 tagacaccaa tcccgaggtt aattttgccg ggacgttcat cggcacgaaa cagatcggcc
     1201 aggcccagaa tcgggtcggc aggagcggcg gtaatgttct caaacatgac gaggttccat
     1261 tatggttaca gaagggaagt ccgctatcag ggtaacggga gatttacaaa attccaacta
     1321 ttactgatga aaacgcaggc tgtttttgca agacgtgaga ttgctctgga aggtataaaa
     1381 aaaacaggac caaagtcctg ttttttcggc atttaacaaa gaggtgtgct attagaactg
     1441 gtaaacgata cccacagcaa cggtgtcgtc tgaacctacg cccagtttgt tgtcagaatc
     1501 gatctggttg atgatgtagt caacataggt ggacatgttt ttgttgaagt agtaggttgc
     1561 gcccacttca aagtagttca ccagatcaac atcaccgata ccttctacgt ctttcgcttt
     1621 agatttggtg taagcgatgg acggacgcag accgaaatcg aactggtatt gcgcaactaa
     1681 cagaacgtct tgcgttttgt tggcgaagcc gctggtgttt gtaaatttat tagtgatcgg
     1741 cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag atgttgttcg cgtcgtactt
     1801 cagaccagta gcccactgtt cagctttttt accgttgcca agaggttgag cttcttgcag
     1861 gttggtacgg tcagctgcac cataagcacc aacgatacca aagccttcgt attcgtagct
     1921 gatagaaccg ccaacaccgt cgccgttaga acggcgtgca gtgtcacgct cgtttttacc
     1981 caggtactga acagcgaagt tcaggccatc aaccagacca aagaagttgg agttacgata
     2041 ggtagcaacg ccgccaacac gaccaacgaa gaagtcatcg ctgtatgcag tatcaccacc
     2101 aaattctggc agcatatcgg tgtaacccag tgcatcataa accacaccgt agttacggcc
     2161 gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg aatgccagac gcgttttgtt
     2221 accagtttga gcgtcagcgc cttcagagtt gttaccctgg aagttatatt cccactgacc
     2281 ataaccggtc agatcggaat tgatttgagt ttccccttta aaaccaagac gggcataggt
     2341 catgtcgcca ttgccaccgt aactgttttc accgttaccc ttggaaaaat aatgcagacc
     2401 aacagcttta ccgtacagat ctactttgtt gccatctttg ttatagattt ctgcagcgtt
     2461 tgcagtacct gctactaaca gagcagggac gatcactgcc agaatattgc gcttcatcat
     2521 tatttattac cctcatggtt ttttttatga cacctgccac tgccttcaat aagttctgtc
     2581 aataaaaatt tacggaacta ttgatgagag tttggtgtct ttatgtgtct gcagg
//
LOCUS       DAPGWD010000281         2632 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPGWD010000281 DAPGWD010000000
VERSION     DAPGWD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.284"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            67..1497
                     /locus_tag="RHX88_004420"
     CDS             67..1497
                     /locus_tag="RHX88_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV2233016.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1538..2470)
                     /locus_tag="RHX88_004421"
     CDS             complement(1538..2470)
                     /locus_tag="RHX88_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDV2233017.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
BASE COUNT          824 a          545 c          573 g          690 t
ORIGIN      
        1 ccgctggaca ttatttcagc ataagtggat attcaaagtt ttgctgtttt atcagggaat
       61 atttatatga tacgtaagtc agctacaggt gttattgttg cgttagccgt aatctggggt
      121 ggtggcacat ggtacacagg tacacaaatt cagcctggtg tcgaaaaatt tattaaagat
      181 tttaacgatg ctaaaaagaa aggtgaacat gcctacgata tgacgttaag ttatcaaaat
      241 tttgacaaag gcttttttaa ttcccgtttt caaatgcaaa tgacattcga taacggtgca
      301 cccgatctca atatcaagcc aggccagaaa gttgtatttg atgtggatgt tgagcacggt
      361 ccgttgccca tcacaatgtt aatgcatggt aatgtcatcc cagcactggc agcggcaaaa
      421 gtgaacttag tgaataatga actgacacaa ccgctattta tcgccgcgaa aaataaatcg
      481 cccgtggaag cgacattgcg attcgcgttt ggtggctcat tctctacgac attagatgtt
      541 gcccctgcag agtatggaaa gttttctttt ggtgagggcc agtttacttt taatggtgat
      601 ggtagttcat tgtctaacct ggatattgaa ggcaaagtcg aagatattgt tctgcaatta
      661 tcaccaatga acaaagtaac ggcaaaaagt tttaccattg attctctggc gcgattagaa
      721 gaaaagaaat ttccggttgg tgaaagcgag tcgaaattta atcagattaa cattatcaat
      781 cacggggaag acgttgccca aatcgatgct ttcgttgcaa aaaccaggct ggatcgcgtt
      841 aaagacaaag attatatcaa tgtcaatctg acctacgaac ttgataagtt aacaaaaggg
      901 aatcagcaac tcggtagtgg tgagtggtca ttgattgctg aatctattga tccctcagcg
      961 gtgcgccaat ttatcatcca gtataacatt gcaatgcaga agcagcttgc tgcacaccct
     1021 gagttagcaa acgatgaagt tgctctgcaa gaagtgaatg ctgcattgtt caaagagtat
     1081 ttaccgttat tacaaaaaag tgagccgacc attaaacaac cggtaaaatg gaagaacgca
     1141 ctcggcgaac taaatgccaa tctggatatc agtattgccg acccagccaa atcttcatca
     1201 tccacaaaca aagatattaa atcgctcaat tttgatgtga agttaccgct taatgtcgcc
     1261 acagaaaccg caaaacagct taatttatct gaaggaatgg atgcggaaaa agcgcaaaag
     1321 cgggctgata aacaaatcag cgggatgatg accttaggtc agatgtttca gttaatcacg
     1381 attgacaaca ataccgcctc gctgcaactg cgttatacac cgggtaaagt tgtttttaac
     1441 ggacaggaga tgagcgaaga agaatttatg tctcgtgccg gacgttttgt tcattaaaat
     1501 actcactcgc ctctgctcat tcagaggcga gcaaaattta cttgtcctgc cgctgtgatg
     1561 acatcaacga cgtcagaagt cggtcttttt cctgccacag tgagttcagc cattgctgaa
     1621 aatggcgctt aaagcttttg tcattgatgt agtcgccatg taactcatcg gcaataggct
     1681 gtaaatccac atgaacgaca attcgcgtca gtttaccgct taacatatcg aagaacggct
     1741 gacggttatt gtccggataa cacagcgtaa cattaagcag tttatcgaat tgtttaccca
     1801 gtacatttag cgccatcgca atgcctgccg cctttggtgg caacaagttt tgaaaagtgg
     1861 agtgggtttg ctgatgtttt tcttgcgtga aacgggagcc ttcaacgaaa ttgacaatgg
     1921 tggtgggatg caggcgaaac ttttcacaag aacgccgagt ggtttcaaca tctttaccac
     1981 gtcgctccgg atgacgtaat aaataagcgc gggaataacg cttcataaac ggcatatcca
     2041 gcgcccagca cgccaggcca agaaatggca cccaggccag ctgttgcttg aggaaatatt
     2101 tattcatcgg aatgtgctta cgaaacagca cgcacagtac gacaatatct gcccaactac
     2161 ggtggttaca aataagcaga taccagttct tcttacttaa cccttccagc ccgtgaactt
     2221 cccattgcag gtgtgggttc agatgcagta ataccgccag accttcacac cagcaataca
     2281 tcataaaatc acaaaaacgc gaaacctttc gccaaatgac tggcacaggt agcaatagtt
     2341 ttacaatccc ggcaatgatg atcgggacgg aacaaaaaat agtgaccaaa atggtcaata
     2401 cgatactcag caaaagggtt atcgcagcga gtattctcgt cataatgaat ttattcaaaa
     2461 ggttagccat agacagtgcg ctaaagaagc gcaaggcgca gattttagca gaaaacgacc
     2521 caaataacgg atgatcctta aggagaaaaa taattcatat ctatccacat tagaaaaaat
     2581 cccattatct caattattag ggatggcttt atttttaact gcatgaaaaa ca
//
LOCUS       DAPGWD010000282         2613 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPGWD010000282 DAPGWD010000000
VERSION     DAPGWD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.285"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..1100
                     /locus_tag="RHX88_004422"
     CDS             48..1100
                     /locus_tag="RHX88_004422"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDV2233018.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1176..2609
                     /locus_tag="RHX88_004423"
     CDS             1176..2609
                     /locus_tag="RHX88_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDV2233019.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT          614 a          584 c          680 g          735 t
ORIGIN      
        1 tgtaaccata tcgcaataag ttatgttttt aaattgaggg cattattatg aaaaaaatac
       61 cctgcgtgat gatgcgaggt ggaacctcga ggggcgcgtt cttgttagcg gaacatttac
      121 ccgaagatca aacgcagcgc gataaaatat tgatggcaat tatgggttcc ggtaacgatc
      181 tggaaattga cggtattggc ggcggtaatc cgctgaccag taaagtcgcc attattagcc
      241 gttccagcga tccgcgtgct gatgtcgatt atctgtttgc tcaagttatt gtccatgagc
      301 aacgtgtcga taccacgcct aattgtggca atatgctgtc tggtgttggg gcattcgcca
      361 ttgaaaatgg tttgattgca gcgacttcac cagttacccg cgtacgtatc cgcaacgtca
      421 atactggtac gttcatcgaa gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg
      481 gtagcgccag aattgacggc gtaccgggta ctgccgcacc ggttgcgctc actttcctga
      541 atgccgccgg aaccaaaacc ggaaaagtct tcccgactga taatcagatt gattattttg
      601 acgatgtccc ggtgacctgt atcgatatgg cgatgccagt cgtcattatt ccggctgaat
      661 atctgggaaa aacaggttat gaattaccgg cggaactgga tgccgacaaa gcattattag
      721 cccgcattga atctatccgt ctacaagcgg gtaaagcaat gggcttaggt gatgtcagta
      781 atatggttat ccctaaacct gtgcttattt ctccagcgca gaaaggcggg gcaattaatg
      841 tgcgttattt tatgccgcat tcttgccatc gcgcgctggc gataaccggt gctattgcta
      901 tttccagtag ttgtgcattg gaaggcaccg tcacccgaca aatcgtccct tctgtaggat
      961 acggcaatat caatattgaa caccccagtg gtgcgctcga cgttcattta agtaatgaag
     1021 gtcaggatgc cacgacgtta cgcgcatctg ttattcggac gaccagaaaa atattttccg
     1081 gtgaagttta tcttccctga aaaaattcgt tgtcaggata aggacaatca ataaaggact
     1141 tctgtatgag tcatacagaa agaacaggat tttaaatgaa taagaaatcg ttatggaagc
     1201 taattctgat attagcgatc ccatgtatta ttggttttat gccagctccg gcaggattaa
     1261 gcgaactggc gtgggtgctt tttggtattt acctggcggc cattgtgggg ctggttatca
     1321 agcctttccc ggaacctgtc gtactgttaa ttgccgttgc tgcctccatg gtggtggtcg
     1381 gtaacttatc cgacggtgcg tttaagacca ccgccgtatt aagcggttac tcttcaggta
     1441 ctacctggct ggtgttctcg gcgtttacct taagcgccgc atttgtgacc accggtttag
     1501 gtaaacgtat tgcctatctg ctgattggta aaatcggtaa caccacgctg ggtctgggtt
     1561 acgttacggt attcctcgat ctggtactgg ctccggcaac accgtctaac accgcgcgtg
     1621 cgggcggcat tgtgttaccg atcatcaaca gcgtggcggt ggctttgggg tccgaaccgg
     1681 aaaaaagtcc gcgtcgtgtc ggacattacc tgatgatgtc catttacatg gtcaccaaaa
     1741 ccaccagcta tatgttcttt accgcaatgg cggggaacat tctggcgctg aaaatgatca
     1801 acgacattct gcacctgcaa attagctggg gtggatgggc actggccgcc ggattgccgg
     1861 gcatcattat gctgctggtc accccgctgg tgatttatac catgtatccg ccagaaatta
     1921 agaaggtgga taataaaacc atcgctaaag cgggccttgc cgaactggga ccgatgaaaa
     1981 tccgcgaaaa aatgctgctc ggtgtctttg tgctggcgct gctgggctgg attttcagta
     2041 agtctctggg ggttgatgaa tccaccgtgg caatcgttgt tatggcgacc atgctgctgc
     2101 tgggtatcgt tacctgggaa gacgtggtta aaaataaagg cggctggaat accttaatct
     2161 ggtacggcgg tattatcgga ttaagctcct tattatcgaa agttaaattc ttcgaatggt
     2221 tagctgaagt ctttaaaaat aacctggcat ttgatggtca cggtaacgtt gctttcttcg
     2281 ttattatttt cctcagcatt atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg
     2341 ttgctatgtt accggtattt gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa
     2401 ccgcgctggc actgttgttc tccaactcct atggcggcat ggttactcac tatggcggcg
     2461 cggcaggtcc ggtcatcttt ggcgtgggtt acaacgatat taaatcctgg tggttggtcg
     2521 gtgcggtact gacgatatta accttcctgg tgcatatcac cctcggcgtg tggtggtgga
     2581 atatgctgat cggctggaac atgctgtaaa tat
//
LOCUS       DAPGWD010000283         2590 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPGWD010000283 DAPGWD010000000
VERSION     DAPGWD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.286"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..2502
                     /locus_tag="RHX88_004424"
                     /pseudo
     CDS             1..2502
                     /locus_tag="RHX88_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          611 a          677 c          907 g          395 t
ORIGIN      
        1 catgcggacg ggagcattac ccgcagcgag tatgatgagg cggggaggct gaaggcacag
       61 acggatgcgg cggggcggcg gacggagtac agcctgcata tggcgtcggg tgcggtgacg
      121 gcggtgacgg ggccggacgg caggacggta cggtatggct ataacagcca gcggcaggtg
      181 acgtcggtga cgtacccgga cgggcttcgc agcagccggg agtatgatga gaagggaagg
      241 ctgacggcgg agacctcgcg cagcggtgag acaacgcggt acagctatga tgacccggcg
      301 agtgagctgc cgacagggat acaggacgcg acgggcagta caaaacagat ggcatggagc
      361 cgttacggtc aactgctggc cttcacggac tgctcggggt acacgacgcg gtatgagtac
      421 gaccggtacg gtcaacaaat cgccgttcac cgggaggaag gcatcagcac ttacagcagt
      481 tataacccgc gtgggcaact ggtcagtcag aaggatgcgc aggggcgtga aatccgttat
      541 gagtacagcg ccgcaggcga cctgacagca acggtatcac cggacggtaa acgcagcacc
      601 atcgaatatg ataaacgcgg gcgtccggtg agtgtgacgg aaggcggtct tacgcgcagc
      661 atggggtatg acgctgccgg gcgcatcacc gtgctgacca acgagaacgg cagccagtcc
      721 acgttccggt atgacccggt ggacaggctg actgaacagc gcggttttga cggccggacg
      781 caacgttacc actatgacct gaccggaaaa ctcacgcaga gtgaagacga ggggcttatc
      841 accctctggc actacgatgc gtcggaccgc atcacgcacc ggacggtgaa cggcgacccg
      901 gcagagcagt ggcagtatga tgagcacggg tggttaacca ccctcagcca tacctgtgaa
      961 ggccaccgcg tgtcggtcca ctacggctat gacgataaag gccgcctgac gggtgaacgg
     1021 cagacggtgg agaacccgga gacaggggag atgctgtggg agcatgagac ggggcatgcg
     1081 tacagcgaac aggggctggc gacccgtcag gagccggacg gtctgccgcc ggtggagtgg
     1141 ctgacgtatg gcagcggtta tcttgcgggg atgaagctgg gcggaacgcc gctggtcgag
     1201 tacacgcggg accggctgca ccgcgagacg gcccgtagct tcggcggggc gggcagtaca
     1261 gcggggtacg aacaggccac ggcgtacacc ctcacagggc agctacagag caggcacctg
     1321 aaccttccgc agcttgactg tgactatacg tggaacgata acggacaact ggtgcgcatc
     1381 agcggcccgc aggagtgccg ggagtaccgt tacagcggca cgggaaggct gacgggcgtc
     1441 cacactacgg cagcgaacct ggatatcgat atcccgtatg cgacggaccc ggcaggaaac
     1501 cggctgccgg acccggaact gcacccggac agcaccctca cggcgtggcc ggataaccgc
     1561 atcgcggaag atgcgcacta tgtctaccgc tacgatgaat acggcaggct ggcggagaag
     1621 acggaccgca tcccggaagg ggtgatacgg atgcacgacg agcggaccca ccactaccac
     1681 tacgacagcc agcaccgtct ggtgttctac acgcggatac agcacggtga gccgcaggtg
     1741 gagagccgct acctctatga cccgctgggc cgccggacgg gaaaacgggt gtggcggcgg
     1801 gagcgtgacc tgacggggtg gatgtcgctg tcgcgtaaac cggaggagac ctggtacggg
     1861 tgggacggtg acaggctgac cactgtacag acccaacaga caagaatcca gacggtatac
     1921 cagccgggaa gcttcacgcc gctcctgaga atcgaaacag agaatggtga acaggcgaag
     1981 gcgcggcacc gtagcctggc ggaggtgttg caggaggaca cgggtgtgac gctaccggcg
     2041 gagctggcgg tgatgctggg gaggctggag cgggaactgc ggcagggcag cgtgagtgaa
     2101 gaaagccagc agtggcttgc gcagtgcggg ctgacggcgg aacagatggc cgcgcaactg
     2161 gaggcggaat acatcccgga gaggaaactt catctttacc actgtgacca ccgtgggctg
     2221 ccgctggcgc ttatcagccc ggaaggggaa acggcgtggc agggggagta tgacgagtgg
     2281 ggaaacctgc tgggcgagga aagcgcgcag caccttcagc agtcactccg tctgccggga
     2341 cagcagtatg atgaggagtc ggggctgtac tacaaccgca accggtacta tgatccgttg
     2401 caggggagat atatcaccca ggacccgata gggctgagag gtgaatggaa tctgtataaa
     2461 tatccattaa accctgttcg gtttatagat tctttgggtt taaaattcca tgttaacggt
     2521 gatccaagtg attttaatca ggctgttgag tatttaaaac aagattcaca aatgaaagaa
     2581 actatagatt
//
LOCUS       DAPGWD010000284         2578 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPGWD010000284 DAPGWD010000000
VERSION     DAPGWD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.287"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(69..>2578)
                     /locus_tag="RHX88_004425"
     CDS             complement(69..>2578)
                     /locus_tag="RHX88_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV2233020.1"
                     /translation="TRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYT
                     TRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIIA
                     PDGSRSEPQYDAWGKAISTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLT
                     EQRGFDGRTQRYHYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQWQYDDH
                     GWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLA
                     TRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSMAGSNAAYE
                     LTSTYTPAGQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRT
                     LAPDLDIRIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEK
                     TDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVW
                     RRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENG
                     EQAKARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTAE
                     QMAAQMEDAYIPERRLHLYHCDHRGLPLALISPEGETAWCGEYDEWGNQLNEENPHHL
                     YQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLEGGWNLYIYPLSPVMNID
                     PMGLSVMAPALSMQNLSALLKSKMESTISSCNGESSGYLLTCQTAYDSDCGEIHERCM
                     MLCKTAQIDPDMSHIWGGSLFGCLNGCLPARCGGNAV"
BASE COUNT          442 a          860 c          670 g          606 t
ORIGIN      
        1 acccggcaga acggagacgg aaaaactcat actgccattc attattaatc aatatgatat
       61 cctcataatt atacggcatt tccaccacat cgagcaggta gacaaccatt tagacaccca
      121 aaaagagaac cgccccaaat gtgtgacata tctggatcta tttgtgcagt tttacataac
      181 atcatacacc gctcatgtat ttctccgcag tcactatcgt atgctgtttg gcaggttaat
      241 aaatatccag atgattcccc gttacaagac gaaattgtcg attccatctt acttttaagt
      301 aatgcggata agttttgcat tgacaatgct ggagccataa cagatagccc catcggatca
      361 atgttcataa ccggactcaa cggataaatg tataaattcc atcccccctc cagcccaatg
      421 ggatcctggg tgatatacct cccctgcaac ggatcatagt accggttccg gttgtagtac
      481 agccccgact cctcatcatg ctgctgcccc ggcagacggt atggctgata caggtgatgc
      541 gggttctcct cattaagctg gttgccccac tcatcatact ctccgcacca cgccgtttcc
      601 ccttccgggc tgatgagcgc cagcggcagt ccccggtggt cgcagtggta aagatgcagc
      661 ctccgctccg ggatgtatgc atcctccatc tgcgcggcca tctgttccgc cgtcagtccg
      721 cactgcgcaa gccatgcctc actctccgca ctcaccgctc ccgcgcgcag ttcccgctcc
      781 agccttccca gcatcaccgc cagctccgcc ggtagcgtca cacccgtgtc ctcctgcaac
      841 acctccgcca ggctacggtg ccgcgccttc gcctgttcgc cattctctgt ttcgattctc
      901 aggagcggcg tgaagcttcc cggctggtat accgtctgga ttcttgtctg ttgggtctgt
      961 acagtggtca gcctgtcacc gtcccacccg taccaggtct cctccggttt acgcgacagc
     1021 gacatccacc ccgtcaggtc acgctcccgc cgccacaccc gttttcccgt ccggcggccc
     1081 agcgggtcat agaggtagcg gctctccacc tgcggctcac cgtgctgtat ccgcgtgtag
     1141 aacaccagac ggtgctggct gtcgtagtgg tagtggtggg tccgctcgtc gtgcatccgt
     1201 atcacccctt ccgggatgcg gtccgtcttc tccgccagcc tgccgtattc atcgtagcgg
     1261 taaacatagt gcgcatcctc tgcgatgcgg ttatccggcc acgccgtgag cgtgctgtcc
     1321 gggtgtagct ccgggtccgg cagccggttt cccgccgggt cggtcgcata cgggatgcgg
     1381 atgtccaggt ccggtgcgag ggtgcgcacg ctctccagcc tgcccgtggc gctgtagccg
     1441 tattcccgcg tctgtcgcgg accgctgatg cgcaccaggt cgccgttgtc attccacccg
     1501 tagtcacggt catacaccag gctgttcagg tgctggctct gtaactggcc tgcgggggtg
     1561 tatgtgctgg tcagttcata tgcggcatta ctgcctgcca tgctgccgaa gctgcgcacc
     1621 gtctcacggt gcagcctgtc ccgcgtatac tcgaccagcg gcgttccgcc cagcttcatc
     1681 cccgcaagat aaccgctgcc atatgtcagc cactccaccg gcggcagacc gtccggctcc
     1741 tgacgggtcg ccagcccctg ttcgctgtac gcatgccccg tctcatgctc ccacagcatc
     1801 tcccccgtct ccgggttctc caccgtctgc cgttcgcccg tcaggcggcc tttatcgtca
     1861 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
     1921 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
     1981 cggtctgacg catcgtaatg ccagagggtg ataagcccct cgtcttcact ctgcgtgagt
     2041 tttccggtca ggtcataatg gtaacgttgc gtccggccgt caaagccgcg ctgttcagtc
     2101 agcctgtcca ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcacg
     2161 gtgatgcgcc cggcagcgtc ataccccatg ctgcgcgtca gaccgccctg cgtggtgctg
     2221 atggctttac cccacgcatc atactgtggc tcactgcggc taccgtccgg ggcaatgata
     2281 gcggtgaggt cgcctgcggc actgtactca taacgggtct cccgcccctg cgcatccttc
     2341 tgactgacca gttgcccacg cgggttataa ctgctgtaag tgctgatgcc ttcctcccgg
     2401 tgaacggcga tttgttgacc gtaccggtcg tactcatacc gcgtcgtgta ccccgagcag
     2461 tccgtgaagg ccagcagttg accgtaacgg ctccatgcca tctgttttgt actgcccgtc
     2521 gcgtcctgta tccctgtcgg cagctcactc gccgggtcat catagctgta ccgcgttg
//
LOCUS       DAPGWD010000285         2572 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPGWD010000285 DAPGWD010000000
VERSION     DAPGWD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.288"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(402..923)
                     /locus_tag="RHX88_004426"
     CDS             complement(402..923)
                     /locus_tag="RHX88_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDV2233021.1"
                     /translation="MNIVPLNYKGEIVSFNTDGWINVTGVAERFGKRIDNWMRLAETL
                     EYVRALDEALTGKESQILHPSQSRYVKTSKARKDRGGGTWLHPKLSVAFARWCDARFA
                     VWCDLHIDSLLRGELTEQQKYEQACRIRDDRKSKASNGAREMARWRWDKPVIEANVEY
                     WREQLQLTLDIAC"
     gene            complement(1128..1565)
                     /locus_tag="RHX88_004427"
     CDS             complement(1128..1565)
                     /locus_tag="RHX88_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDV2233022.1"
                     /translation="MNRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRDDVSAGRKRLQVAASCAKSKTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            complement(1562..2059)
                     /gene="rrrD"
                     /locus_tag="RHX88_004428"
     CDS             complement(1562..2059)
                     /gene="rrrD"
                     /locus_tag="RHX88_004428"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDV2233023.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(2059..2274)
                     /gene="essD"
                     /locus_tag="RHX88_004429"
     CDS             complement(2059..2274)
                     /gene="essD"
                     /locus_tag="RHX88_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV2233024.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          673 a          648 c          559 g          692 t
ORIGIN      
        1 attactcttt actgagaaca ttcagaacac tacacaaata tttccgcact aaatcataac
       61 gtccggtttc ttccgtgcca gaaccggact cgctggcatg atgaaatatg tgtacccggt
      121 aaccccggtg tgcatcgttt ttgattattc ccgcacactc gcgcagaagg agttccccgt
      181 cgggctacgg tctctgttaa tacgggaata cggcgacgat acagcgcatg atgtgtcagg
      241 cttgaatgcc tttatccgtt aaaagggata tcagttaagt tatcccgtgc agggtataag
      301 ccattatcaa gcccacccgt agatgggctt tgtaatggat agccgttgct cagttctagt
      361 aatgctttga ttttttcgat aacgcagttt tgcgtttgcc atcagcacgc gatatcgaga
      421 gtcaactgca gttgctcgcg ccagtactca acatttgctt caataaccgg cttatcccat
      481 cgccagcgag ccatctctct tgccccattg ctggcttttg atttccggtc atcgcgaatg
      541 cgacatgctt gctcatattt ctgctgctca gtcagttcac cgcgaagcag actatcaatg
      601 tgcaggtcgc accacacagc aaaacgagca tcacaccaac gggcaaatgc aactgaaagt
      661 tttggatgta gccacgtacc accacccctg tcctttcgtg ccttgctggt ttttacatac
      721 ctcgattgtg agggatgtaa aatttgagat tctttcccgg tcaacgcttc gtctaaagca
      781 cgaacgtatt caagcgtttc tgccaaacgc atccagttat caatgcgttt cccaaatctc
      841 tcagcaacac ctgtgacgtt gatccaacca tcagtgttga aactgacaat ttcacctttg
      901 taattaagtg gcacgatatt cataacgttt acctaccatt tgaaatgaac ctttgccgca
      961 caggaaacca gcccaccgag gctcgccagc actaactggt atcctcaaag gcccattcca
     1021 aaggggcagg ttcggtgtaa aaaaaacatg cgttgcggta cgcatttatt gcaaaaaagc
     1081 cccgcatcgc gaggctcatt aaatggactt tgtgatttgc aaaaaaatta tttcagacat
     1141 tgcgtcctga tgtactcctg caggtagtta acctgcgcgg ttatcctgtc gattccactt
     1201 cggagacggt aataattgag ttcagcatct gctgtaagtc ttgggctttc tccatcgccc
     1261 atgctgctgg ctccggtctt tgactttgca caggaggcgg cgacttgcag ccgcttacgc
     1321 ccagcagaaa catcatcacg gagactttcg atagtcgcgt tagcatcagc aagctccttt
     1381 gtatatctgg catcgagttc tgctacgtca cgttgacgct tctgcatgtc agcgatgatg
     1441 gatgtggctt tgtcgcgctg ttctttgtag gtcatggcgt tatcacggta atgattaaca
     1501 gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc ggttactctg
     1561 ttcattgctg accccacaaa cagatttcac gctcaatctc acgacgagtc atgagacctt
     1621 tccattgctt accgccagca tatgtccagc gacgtagctg atcacatgcg cctttgatat
     1681 cgccctggtt tattttgcga agaagcgtcg atgttctgaa attgccagca cccacgttgt
     1741 aaacgaatga gtaaagagcg ccgcgcattg tttccggtat atcgactttg atgtacgggt
     1801 taatttgtct ggcgaccgtg gcaaggtctt tattcaggag ggctttgcat tctgctttgg
     1861 tatacgtttt accgagcatg atgtcttttc ctgtatgccc gtgacataca gtccatacac
     1921 caacaatatc tttgtatggt atgtagctga caccttccag accatcgtta ccacttgggc
     1981 cagtaattaa cactgatgct atagcaattg ctccgccacc aatagcagca gcaacggctt
     2041 ttcgtaatga tggaggcatt attcacctct cgcagccttg cgcttatctt ctttaatctt
     2101 gaaataaagg tttgtcaggt acgtcagcag gccaaatacc aggctaccca gcacacctat
     2161 tgctgcccac tgtgagggcg tgactttatc gagcagctgt aaaaaccagt aaccggcact
     2221 acctgctgag gtgccatagg cgacacccgt tgttaactta tccatggatt tcataacccc
     2281 acctcgcaga caaagcgggt gtaaattgag ggaatacaac gtatcgcaaa aaagcagaaa
     2341 cgtaacagac tcggagtcag tgaataactc aggtattgag ttatcagcta atatcgagac
     2401 tcaaaaaatg gaaaaaccag ctcgacggcg ggtttaagct gtgtgacgaa gtaaccactc
     2461 ttaacagcat aaccaatttt ttacgtacgt aaaccactga atgatattta tgagaatgct
     2521 accgagtgtt caaaacacca ccacaaatac ataagaaaac ctcaacaaat aa
//
LOCUS       DAPGWD010000286         2570 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPGWD010000286 DAPGWD010000000
VERSION     DAPGWD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.289"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            426..569
                     /locus_tag="RHX88_004430"
     CDS             426..569
                     /locus_tag="RHX88_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDV2233025.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            582..791
                     /gene="cspE"
                     /locus_tag="RHX88_004431"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             582..791
                     /gene="cspE"
                     /locus_tag="RHX88_004431"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV2233026.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            957..1766
                     /gene="rlmA"
                     /locus_tag="RHX88_004432"
     CDS             957..1766
                     /gene="rlmA"
                     /locus_tag="RHX88_004432"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDV2233027.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1763..2329)
                     /gene="mntP"
                     /locus_tag="RHX88_004433"
     CDS             complement(1763..2329)
                     /gene="mntP"
                     /locus_tag="RHX88_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDV2233028.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          678 a          607 c          615 g          670 t
ORIGIN      
        1 tttttttgct cattcatcaa tttttcttat tttaaatttt caatcccttt ggggattgac
       61 ttctctttag ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac
      121 tttttattca ttgcatagca aaaaatgtga tattgcacgc actatgtaat aacttctccc
      181 actggcctgg aacaactgaa cttattgaac tatgttagaa aatacgccag tttaagtatc
      241 tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg
      301 tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat
      361 ggcttgccat aattaacgtt gtatgtgata acagatttcg ggttaaacga ggtacagttc
      421 tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc
      481 gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt
      541 tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc
      601 aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca
      661 aagatgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag
      721 gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa
      781 cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag
      841 cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt
      901 gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgccgga gttgtcatgt
      961 cgttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc
     1021 cccagcgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc
     1081 ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat acaagcacgc cgcgcattct
     1141 tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc
     1201 ttgatgaaaa ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg
     1261 catttgccga tgtgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga
     1321 taaaagcggc ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt
     1381 tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag
     1441 aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc
     1501 gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg
     1561 cagaacaact ggaaggtttt acattacagc agagtgatga gttgtgttat ccgatgcgtc
     1621 ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc
     1681 cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata
     1741 ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga
     1801 tgccgatcag caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa
     1861 agcgaccaac catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg
     1921 cggtcgcgat aatgttgacc tgcaggaaag caagaccaac acccacagcc atggcatcca
     1981 ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgtcgacgg cgcggctctt
     2041 catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca
     2101 gcagcacaaa cgcaatccag tggttccatt caaggacaaa ccggctggct aacatgccca
     2161 ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca
     2221 atgcttcaga aaatttcggt ttatggaggg tggcaccttt accgattgat gcagcaaatg
     2281 catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg acaatgtcct
     2341 gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa
     2401 gccgtgatgg ttttcgcgtt catgcaggca tagcgattga tgtgcatatg gtctcgcctg
     2461 actgaaagta ttcagtccgt acgtgccacg ttttgcaacg agtatgttga cacgtacatt
     2521 tccggaatct ggaaatcagc tactccccaa atgagggcgc aaccttaaca
//
LOCUS       DAPGWD010000287         2566 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPGWD010000287 DAPGWD010000000
VERSION     DAPGWD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.290"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..1061
                     /locus_tag="RHX88_004434"
     CDS             81..1061
                     /locus_tag="RHX88_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HDV2233029.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            1054..1470
                     /locus_tag="RHX88_004435"
     CDS             1054..1470
                     /locus_tag="RHX88_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HDV2233030.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(1472..>2566)
                     /gene="umuC"
                     /locus_tag="RHX88_004436"
     CDS             complement(1472..>2566)
                     /gene="umuC"
                     /locus_tag="RHX88_004436"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HDV2233031.1"
                     /translation="MRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDEMF
                     IDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFSGV
                     VALTAENRNRILKLLGLQPVGEVWGVGHRLTEKLNALGINTALQLAQTNTAFIRKNFS
                     VILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAAEK
                     LRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHVWR
                     EGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFAGR
                     GTAPEWQMKREMLSQCYTTKWRDIPLARLG"
BASE COUNT          652 a          577 c          613 g          724 t
ORIGIN      
        1 tttcattata attgaatata cattgcatgt atgaagtatg tttgtttttt ctatttgttt
       61 atgagcaacg gagactggtt atgaaaatct ttattgatga tggttctacc aacattaaac
      121 ttgcctggtt ggaggatggt gacgtgaaaa cgttaatcag ccctaacagc tttaaaccgg
      181 aatggtcttt tagtttactg gatgatgctg ctccggctaa ttacgagatc gacggcgaga
      241 agttttcatt tgatccatta agtgctgatg ctgtcgtaac gactgaaaca cgctatcagt
      301 acagtgatgt gaatgttgtg gccattcagc atgcgctaca gcagaccggc ctgaaagcgc
      361 agcctgttga tgtcatcgtc acattgccga tctctgaata ccttgacgcc aacaatcaga
      421 aaaataagca aaacattgaa agaaagaaaa aaaatgtgat gcgtgaagta cgggtgcagg
      481 ggagtgatgc atttgttatt cgcagtgttt ccgttcttcc tgaaagtatt cccgctggtt
      541 tcagtgttct tgcggggctt gaggatgatg aatccctttt gatcgtagat ctcggtggta
      601 caactcttga tgtgtcgcat gttcgtagca aaatgacagg gattacaaaa acctggtgtg
      661 atccgaatat cggcgtgtcc ctgattacat ccggggttaa ggaacagatg gctgtacatg
      721 ctaatacaag agtgagctca ttccaggctg acaatatcat tgtgcaccgt aatgaacctg
      781 actacctttc ccgtcgcatt tataatgctg aacagcgtga gtctatcatt aatgttatca
      841 atgaacggca gaagttgctt attaaacgag ttaatgatgt catcagccgt ttcaccgact
      901 atacacatgt aatgtgtgtt ggtggtgggg ctgaaattgt ggccgaggct gtgaagaacc
      961 tgactaaggt tcctgatgag cgcttttatc tgagctcatc cccgcagttt gatttagtca
     1021 tgggaatgat aaaaatgaaa ggtggtgtga ctaatgagtg atgaaaataa atccagacgc
     1081 tgctcatttg aacttttccc ggatgaacgg acaggagata agatagcaga tgagctcatt
     1141 gcaaatgaaa aactgaagga gcggggacgt ttcatgcgcg cgatgttagt tactggtgcg
     1201 gcgtttgcag ctatagataa acgtcttcct ttgctgatat cggagttact gacagaaaat
     1261 acgactctgg atgatattaa taaggtgatt tccagcgtaa taccaggtgc tttttcggtt
     1321 gaaaagaaat tgcttgaatt actggaaaaa caatctggtc tccatacgtc tgtggattgc
     1381 agtactcctc ttacagagca gagtctgtcg agaaatgacg gtgaagacca gaccagaaga
     1441 aacgctgaaa atatgttcgg agatgactga actaacccag cctggccagg ggaatatctc
     1501 gccatttagt tgtataacac tgactcaaca tttcccgttt catttgccat tcaggggcgg
     1561 ttcctcgccc cgcaaaccac actttccctt ttcccgactg gttcagttca tcgagtgttt
     1621 tcattaactt ttcactgttt ttacggggct ggatttcatc aaataatccc ggctgcgcta
     1681 tacccgatgg tgtgaaatca gccagcatga cacctgcctt catatagcgg tacccttcac
     1741 gccagacatg gtttaaggct ctgcatgcgg cggcaataat gtcccggctg tcctgtgtgg
     1801 gcaatggaag cttttccaca gcggcattgc tgtaacaggg ttcttttact gcaaagggtg
     1861 atgtccgtac aaatgtcgtc acctgccggc aatactgacg ctccccacgt agtttctctg
     1921 cggcccgctc tgcatactga acaacagcct ggtgcatggc atctttgtct gtgattcgtt
     1981 caccaaaact gcgactacag acaatctgct gttttgccgg tggtgcttct tccagggata
     2041 tgcaggactc gccgttgagt tcgcgtaccg tacgctcaag aatgacgctg aagtttttcc
     2101 ggatgaatgc cgtgttagtc tgcgccagct gcagtgctgt gttaataccc agcgcattca
     2161 gcttttccgt cagtctgtgt cctactcccc agacctcacc aactggctgc agccccagta
     2221 gcttcaagat ccgattacgg ttttctgccg tcagcgcgac cactccggaa aactgtggcc
     2281 attgctttgt tgcccactgt gcacttttag ccagcgtttt tgtaggcgca atgcccaccc
     2341 ccatggtgag tcctgtccag ctctttacct gttccctgag ctgatgacca aaaaactccg
     2401 gagagatgca atgatttatc ccccgcaaat caatgaacat ttcatcaatt gagtagggct
     2461 caactgcggg agaaagcgac tccagaacag ccataacccg ttggctcatg ctgtggtaca
     2521 gcgcataatt gctggaaaat acatgtattt tcttctccag gcgcat
//
LOCUS       DAPGWD010000288         2503 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPGWD010000288 DAPGWD010000000
VERSION     DAPGWD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.291"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            640..867
                     /locus_tag="RHX88_004437"
     CDS             640..867
                     /locus_tag="RHX88_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233032.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(902..1042)
                     /gene="ykgO"
                     /locus_tag="RHX88_004438"
     CDS             complement(902..1042)
                     /gene="ykgO"
                     /locus_tag="RHX88_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDV2233033.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1042..1305)
                     /locus_tag="RHX88_004439"
     CDS             complement(1042..1305)
                     /locus_tag="RHX88_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDV2233034.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1669..1770)
                     /locus_tag="RHX88_004440"
     CDS             complement(1669..1770)
                     /locus_tag="RHX88_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233035.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
BASE COUNT          739 a          454 c          556 g          754 t
ORIGIN      
        1 tgtttttttc ccgatgagtg tcaatatgtg aacggaaaag gaattcaatg gttaagtcaa
       61 tatttagaaa aactgaaaat attgcttttt tgataagaag aaaaagacat ttggtttcat
      121 aaggttattg gcaatataac gttcaattct tacttgcatt ttatattggt caggaacttt
      181 gccctgaagt gttaggaata gtcttaatgc gctgtgtgat ggactagtaa aaaaacaagt
      241 tttaagggta agggatagaa tataaaacta taatgagctg tcattgaaga tgatttgcca
      301 atcagtaaga aattttaatg gattaacatg ccgcacaaaa tggttatagg tgttcattac
      361 aataagtgat ggaaaattga acgggcgtat gtacgtcatc tgttcggtaa gcccccaaga
      421 gccagattaa gtgaagttaa acgaaggctg ccagctgtca caggaacgat ggcctctttg
      481 tcagggcact gagtgcagtt atctgtgcgc gttactctaa ctggcactcg aacgtcggcg
      541 acggggttac cggtggatca gactgttcct gtggcgtaaa cgttttatgt tagtaccaac
      601 agatttacca catttatcat cttaacagtt tgagtgaaaa tgcaggctgc gccgcaaagg
      661 gaggagactg agtggcgtgt tcagtctaaa agagagttga tgccagcata caggggcgaa
      721 gctggtcaac aagtcaacat taagataatg gaatattcgg agagaaacgt caggcagttg
      781 gcctcaaatg aacaggaaga atatatcccc aggaaaatta atgttggtgt tataaacaca
      841 ccaacattaa tccgatctga ttattaatca gacaaatatc gttaatccgt cgaattttga
      901 atcaacgttt tttcttacga ccctgaacgg ccttaaaacg tggattagat ttacaaatca
      961 catatagccg tccttttcgc ttcacaatct gacagtctgg atggcgttct tttgcggtac
     1021 gcagagagtt aaggactttc atcatgcccc ctttttcgtg ctaacaaaac gaccaaaacg
     1081 ttgggtgaac cgtgcaacat ttccttctga tgccactgtt ctcagcttcc ctgtatagaa
     1141 cgggtgcgat ttcgaagaga catcaattgt tacgtatgga tacgttacgc catccagctc
     1201 aatctcacgg tctgttttga tagtcgagcc gattttaaag tactcatcaa tactggtgtc
     1261 gtggaacacc acagtacgat actcaggatg gatattgggc ttcatcattt ttacctgtta
     1321 tgttataaca taaccataaa gatacatgct catccggatc atttcaacac ccaatccctg
     1381 tgaccattag tggtatgtgg aatttccgca acggggaagt ctggcattgt tagcagccgt
     1441 tggcgacgct cccataaacg cctggatgag tgattgatga tggtttcgct gagagtatgg
     1501 atttttcgac aatggaggtt atctccactt tactggccga tgatttatcg cagtatgatt
     1561 gattacctga cagcgtgtat tcctctccca cggatataat aattacttac aaaaagggag
     1621 aggatgcata ttttaaatat cactgaagtg aacagtttat tgccgttatt aatagaaatg
     1681 gagaaataaa taggcgtatt ctacaattgc gacaaaaaca acgatattaa tcagtttatg
     1741 actgatttgt tgtactttat tctctttcat tggtacttcc tcgctttaaa aaagagtgca
     1801 cttcgtaagt gcccttatat aaataacgag tttggtcaac caattttttg acatgtatca
     1861 caaatttgaa tagatgtatt acatcggcta tcttttattg caccaacgtc attgatatat
     1921 gtcgcctgaa gtcagttccg ggaatgagtc tgatctcaag actggcccag tccgggcgtt
     1981 gattggtgct gaggagtata tcgcatctca tcataatgtc gtatctcctg gggtgttata
     2041 caagatatcg ttgttggtaa cctgggcgag gaattgagtt ctattaaacc gtcaactatg
     2101 ccagatacat actggattac actgcaggca cgccttacga gagaacgtgc cgcagtgacg
     2161 ggttaattat ctgaaagaat ttgcgaggct gtatcggtta ctcattggtt tgatagtttt
     2221 actctcggga gagtaataga tatttaattc attaacggaa accagtcagt tcctttcgat
     2281 gcctgaattt gatcccatag tttatccgga atggttaatt catgaacaag ttgtgttatc
     2341 gttcatgaga agcataacgt aaagggaaaa gctcgattag acggcaggat ttgtcagggg
     2401 ttatgaacga aattcataaa tctgtttgag tgttgcgatg ggtagtgcaa gttcgatatc
     2461 tccgcaattt acagtctgat gaatgaaaat gaatatccat aaa
//
LOCUS       DAPGWD010000289         2441 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPGWD010000289 DAPGWD010000000
VERSION     DAPGWD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.292"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            91..579
                     /gene="eco"
                     /locus_tag="RHX88_004441"
     CDS             91..579
                     /gene="eco"
                     /locus_tag="RHX88_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDV2233036.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(728..2374)
                     /gene="mqo"
                     /locus_tag="RHX88_004442"
     CDS             complement(728..2374)
                     /gene="mqo"
                     /locus_tag="RHX88_004442"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDV2233037.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQFKPQPVQKEVADIAL"
BASE COUNT          578 a          669 c          610 g          584 t
ORIGIN      
        1 tcataaagca aattaataaa tctgatgaat atgttaacct tcagcgacat catcggtgaa
       61 aacctataaa tgaagaagga aagcaaaaaa atgaagacca ttctacctgc agtattgttt
      121 gccgctttcg ctaccacttc cgcctgggcg gcagaaagcg tccagccact ggaaaaaatc
      181 gcgccttatc cacaagctga aaaagggatg aagcgtcagg tgattcagtt aaccccgcaa
      241 gaagatgaat ctaccctgaa agtagaactg ttaatcggtc agacgctgga agtcgattgc
      301 aatttgcatc gtctcggcgg gaagctggaa agcaaaacgc tggaaggctg gggctatgat
      361 tattatgtct ttgataaagt tagctcgccg gtttccacga tgatggcctg cccggatggc
      421 aagaaagaga agaaatttgt caccgcgtat ctgggcgatg ctggaatgct gcgttacaac
      481 agcaagctgc cgatcgtggt gtatacgcca gacaatgtag atgtgaagta ccgcgtctgg
      541 aaggcggaag agaaaattga caacgcggta gttcgctaaa atgctgtgaa gtgcggcacc
      601 ccgtaggccg aataaggcgg tcacgccgca tccgacatct aacgcccgag ccggtgcctg
      661 atgcgacgct ggcgcgtctt atcagcatac gccacatccg gcataccatg ccggatgtgg
      721 cacatcatta caacgcaata tccgccactt ctttttgaac gggttgcggt ttgaactgcg
      781 gttttggcgt actatctgct gcctgcggct tgtcgtattt cagccccagc acttcgctgg
      841 tgtactgcaa ctcgcgttct gttgccgcta catcaccgtt cagcttgcgt ccataagacg
      901 gaacgatcgc tttcaacgta gcctgccatt gcgggctgga aacgcgatcg ccaaatactt
      961 tttccagcag atccaacata atcggcgcgg cggttgacgc ccctggcgat gcccccagca
     1021 gcgcggcaat ggtcccttgc tggtcactga ccacttcagt acccagacgc agtacgccac
     1081 ctttctcggc atcacgcttg ataatctgca cgcgctgccc cgcttgccac aaacgccagt
     1141 cctctttttt tgcctgcgga tagtactctt tcaacgcttc aaaacgatcc tcttcactca
     1201 acatcacctg actcaccaga tatttcacca gatcgaaatt atccagcccg acgtgcatca
     1261 tcggcatcac gttagaggtg gtggtggaac tcatcagatc ccacaatgaa ccgtttttga
     1321 ggaatttggt tgagaaggtg gcaaatggcc caaacagcac tacgcgttta ccgtccagaa
     1381 cgcgggtatc gatatgcgga accgacatcg gtggtgcgcc aacggatgct ttaccgtaaa
     1441 ccttcgccag atggtgatta accacgtccg ggttttccga aacaaggaac tgtccgccca
     1501 ccgggaaacc ggcgtagtct ttcgcttccg gaatccccga ttcctgtaat agcttcagcg
     1561 ccgcaccgcc cgcgccgata aagacaaatt tggcgcggat gttctgtgca gtgccgtttt
     1621 tcagatcggc aacggtaacg gtccaggtgt tatcgtcatt acgctttagg gcgcggactt
     1681 cgctgctgag ttgcagcgag aagttagatt tcttctgtaa ggaagcaatt aactggcggg
     1741 tgatctcgcc atagttcaca tcagtgccaa tttctgtgcg tgttgccgct actttctgtt
     1801 gtggatcgcg cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag
     1861 agtaacgcat accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga
     1921 aattaacgtt atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac
     1981 gcggagtacg cagcacgccg cgctcaacct ggtgcgccca gaactggcgg gaaatctgaa
     2041 atgcttcgtt aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg
     2101 tgtagttcag ttccatcagt gcagaatgcc cggttccggc gttattccag ccgttcgaac
     2161 tctcctgcgc gacaccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct
     2221 cgcgtaaata ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag
     2281 tttcctgctc ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg
     2341 ccatcgagaa gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc
     2401 tttattgcag gtgagatttg cgcggcatca acggtaacac t
//
LOCUS       DAPGWD010000290         2305 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPGWD010000290 DAPGWD010000000
VERSION     DAPGWD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.293"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..677
                     /locus_tag="RHX88_004443"
     CDS             48..677
                     /locus_tag="RHX88_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV2233038.1"
                     /translation="MCEHLITVVEKYGAAERVHLGKQAGNVGRAVTKLPLMGKSLHKT
                     IERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGA
                     YDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLDVACARKPDHKRE
                     SLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(726..2225)
                     /gene="pitB"
                     /locus_tag="RHX88_004444"
     CDS             complement(726..2225)
                     /gene="pitB"
                     /locus_tag="RHX88_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDV2233039.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLTV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          612 a          596 c          569 g          528 t
ORIGIN      
        1 ctccattaat tgccgttatt ggtagcgatg gttcaggcaa gtcaacggtg tgtgaacatc
       61 tcattaccgt tgtcgaaaaa tatggtgctg ccgaaagagt tcatttagga aaacaagccg
      121 gaaatgtcgg tcgtgcagtg acaaaattac cgttgatggg aaaatcctta cataaaacaa
      181 ttgaacgaaa tcaggtgaaa acagcaaaaa aattgccagg accagttccg gcgctggtaa
      241 ttacagcgtt tgtcgcccgt cgcttactgc gctttcgtca tatgcttgcc tgtcgtcgtc
      301 gcgggttaat tgttctaacc gaccgttatc ctcaggacca aattcctggc gcttacgatg
      361 gtacggtgtt cccacctaac gttgaaggtg gtcgttttgt ctcatggctg gcaagccagg
      421 aacgtaaagc gtttcactgg atggcgagcc ataagcctga tctggtcatc aaactcaatg
      481 tcgatctaga cgtggcctgc gcgcgtaagc ctgaccacaa aagggaatcg ctggcaagaa
      541 agatagccat aacgccacag ttaacctttg gtggtgcaca actggttgat atcgatgcca
      601 atcagccact ggaacaggtg ttggttgatg cagaaaaagc gattacggat tttatgaccg
      661 cgcgtggtta tcactagtca aaaatggaaa tgcccgatcg ccaggaccgg gcattttcag
      721 gaaggttaaa tcaactgcaa tgctatccag tacagcccac cagaaagaaa aattgccgcc
      781 ggtaaagtaa acacccacgc catcaggatg ctggttaccg ttttacgctg taacccaccg
      841 ccgtccacca ccatcgtccc tgctaccgca gacgagagga cgtgtgttgt ggagacaggc
      901 atcccaatat aactggcaag gccgatagac actgccgccg tcatttgtgc cgccatgcct
      961 tgcgcatacg tcatgccgcg cttaccaatc ttctcaccga tggtcatcgc tacacgacgc
     1021 cagccaatca tggtgccaat gccgagcgcc agtgctaccg ccatgatgat ccacaccgga
     1081 gcgtactcaa tggtgcttaa catatcgctg cgcagttttt tcagcaggtt ctggtcttct
     1141 ttactgacgc ctggcagttt cgccagcttc gcggaggtat cagagatgca cagcataatg
     1201 cggcgcagct ggctgcgctg actcacggtt aacggctcgt aactttccat attgcctggc
     1261 agcatcgttt taacgcgcgc aatcgcatca aaggtatttg ccggatgaca gtgaaactct
     1321 gttacttgcg tgccatcagt cgatgctgca ggcaatggag gttccatcgc aatcaacttc
     1381 tgcggcagtt caggatgctg ttgcaggtag tgttcgaagt tggtgacggc atcgcgggta
     1441 cgggtaattt catagccgga cgcgttcata ttgacgacga agccagcagg ggcaatcccc
     1501 accagtacca gcatcaccag gccgatccct ttttgtccgt cgttcgcgcc gtgcgaaaac
     1561 gccacgcccg cagcggaaac aatcagcgca atacgcgtcc agaatggcgg tttacgtttg
     1621 ccttttttct ttttgcgatc ttccggaatg cggtgaatac ggtcacgctt tttcgtcccg
     1681 ctccagtagc gtcgcagcag gaatatcagg cctcccgcaa tgaccaggcc gacgataggg
     1741 gaaacaatca gcgaggagaa aattttggtc acttcacgca ggtttaacgc atccatcacc
     1801 gatgagccgg ttaacagcgc gttggttaaa ccgatgccga taatcgcacc aatcaaggtg
     1861 tgcgaactgg aggccggtaa accgaagaac cacgttccca ggttccagat aatcgccgcc
     1921 agcagcatgg aaaagaccat cgccaggccg tgggttgacc ccatattcag caacaaatcg
     1981 gttggcaaca tatggacaat ggcataggca acgctaagtc cgcccaataa cacgccaaaa
     2041 aagttaaaaa atgccgccat caccacagca agttgtggtt gcatggcacg agtataaata
     2101 acggttgcca ccgcattcgc cgtgtcatga aaaccattga ttgcttcgta gaacaacaca
     2161 aatgccagag caagcaataa caaaagccct gtgtatatat caaggccaac aaataaattt
     2221 agcatatatt agattacgcc attttgaatt tacgaacgga cgcattatca gtgactttaa
     2281 cggcatgggc aaagtgaaat atcat
//
LOCUS       DAPGWD010000291         2244 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.294, whole genome shotgun sequence.
ACCESSION   DAPGWD010000291 DAPGWD010000000
VERSION     DAPGWD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.294"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            50..553
                     /locus_tag="RHX88_004445"
     CDS             50..553
                     /locus_tag="RHX88_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2233040.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(599..1015)
                     /gene="rraB"
                     /locus_tag="RHX88_004446"
     CDS             complement(599..1015)
                     /gene="rraB"
                     /locus_tag="RHX88_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDV2233041.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1177..2181
                     /gene="argF"
                     /locus_tag="RHX88_004447"
     CDS             1177..2181
                     /gene="argF"
                     /locus_tag="RHX88_004447"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDV2233042.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVVTLSK
                     "
BASE COUNT          573 a          534 c          567 g          570 t
ORIGIN      
        1 agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta tgaataacat
       61 tgcgccgcaa tcacctgtaa tgcgtcgcct gacgcagcag gataatcccg ccatcgcccg
      121 cgtcattcgt caggtatccg ccgaatacgg tcttaccgct gataaaggct acaccgtcgc
      181 cgatccgaac ctggacgagt tgtatcaagt gtatagtcag ccgggtcatg catattgggt
      241 ggttgagtac gatggtgaag tggtcggcgg cggtgggatt gcgccattaa ccgggagtga
      301 gtcggacatt tgcgaactgc agaagatgta ttttcttccg gctattcgcg gtaaagggct
      361 ggcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct tcaaacgctg
      421 ctatctggaa acgaccgctt ttttgaaaga agcgattggt ttgtatgaac atttaggttt
      481 tcaacatatc gactatgcac ttggctgcac gggccatgta gattgtgaag tgcggatgct
      541 acgcgaactc taaaaaaatg cccgctagtg tgtgctatcg ggcattttaa tcaattaatt
      601 agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgccg tcttcgccat
      661 tgggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc tccgccagcg
      721 tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac tcgctgagga
      781 tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga tcggtcactt
      841 cgtaaccgag tttaaacgct tcaactgctg ctttttccag ggtttccaga tcgtctgcgg
      901 aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc agtaattctt
      961 caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt gccatggctc
     1021 attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat tgcctccacc
     1081 tttgtgacaa agatttatgc tttagacttg caaatgaata atcatccata taaattgaat
     1141 tttaattcat taaggcgtta gccacaggag ggatctatgt ccgggtttta tcataagcat
     1201 ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca gttagccgcg
     1261 aagctgaaag ccgataagaa aagcggcaaa gaagaagcca aactcactgg taaaaacatc
     1321 gcgctcatct tcgaaaaaga ctcgacccgt acccgatgct ctttcgaagt tgccgcatat
     1381 gaccagggtg ctcgcgttac ttatctcggc ccaagcggca gtcagattgg tcataaagag
     1441 tcgattaaag acactgcccg cgtgctgggt cgcatgtatg acggtattca gtatcgcggc
     1501 tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt atggaacggc
     1561 ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat gcaggagcat
     1621 ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc acgtaacaac
     1681 atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg tctggtcgcg
     1741 ccacaggcat gctggccgga agctgcgctg gttacggaat gccgcgccct ggcacagcaa
     1801 aatggcggga atattacgct gacggaagat gtagccaaag gagttgaagg tgctgacttt
     1861 atctataccg atgtatgggt gtcgatgggg gaagcaaaag agaaatgggc agaacggatt
     1921 gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg taacccggag
     1981 gtcaaattcc tccactgcct gcccgcgttt catgacgacc agacgacgct tggtaagaaa
     2041 atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt cttcgaatct
     2101 gccgccagca ttgtttttga tcaggcggaa aaccgcatgc ataccatcaa agcggtgatg
     2161 gtcgtgacgc tcagtaaata attgatatcg ctataaagat gccgggaaat acaaaaacat
     2221 cccggcatca cattatttta tgca
//
LOCUS       DAPGWD010000292         2215 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPGWD010000292 DAPGWD010000000
VERSION     DAPGWD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.295"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..268
                     /locus_tag="RHX88_004448"
     rRNA            <1..268
                     /locus_tag="RHX88_004448"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            364..479
                     /gene="rrf"
                     /locus_tag="RHX88_004449"
     rRNA            364..479
                     /gene="rrf"
                     /locus_tag="RHX88_004449"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(593..1036)
                     /locus_tag="RHX88_004450"
     CDS             complement(593..1036)
                     /locus_tag="RHX88_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV2233043.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            1193..2122
                     /gene="metA"
                     /locus_tag="RHX88_004451"
     CDS             1193..2122
                     /gene="metA"
                     /locus_tag="RHX88_004451"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDV2233044.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          542 a          593 c          543 g          537 t
ORIGIN      
        1 tgaggggggc tgctcctagt acgagaggac cggagtggac gcatcactgg tgttcgggtt
       61 gtcatgccaa tggcactgcc cggtagctaa atgcggaaga gataagtgct gaaagcatct
      121 aagcacgaaa cttgccccga gatgagttct ccctgactcc ttgagagtcc tgaaggaacg
      181 ttgaagacga cgacgttgat aggccgggtg tgtaagcgca gcgatgcgtt gagctaaccg
      241 gtactaatga accgtgaggc ttaaccttac aacgccgaag gtgttttggc ggatgagaga
      301 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat
      361 ttgcctggcg gccttagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa
      421 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca
      481 tcaaattaag cgaaaggcca tcagtcacga tggccttttt gcgttggtga aaaaatgccg
      541 ggtgcgacgc tggcgcgtct tctccagcct gggcaggcac ggcaggggcg gcttattccc
      601 ccacatacgc cagattcagc aacggatacg gtttccccaa atcgtccacc tcagagcgtc
      661 ccgtaacctt aaaacccacc ttcttataga acccaaccgc ctgctcattt tgctcattaa
      721 cgttggttgt cagttccggt gccatcgaga gcgcatgctc caccagcatc cgacccacgc
      781 cgcagccgca cacatcagga tcgataaaca gcgcatccat atgctgccca cttaacaaca
      841 taaacccaac cggctgatcc cgttcattaa ccgcgaccca caacggtgct tccggcagaa
      901 aggaacgaac cagctcttcc agctcggccc gatactctgc tgataaaaaa tcgtgggtgg
      961 catcgacaga tcgacaccaa atcgcaacga gttcctcccc ttcctcatgc cgtgagcggc
     1021 gaatactaat aaccattttc tctcctttta gtcattctta tattctaacg tagtcttttc
     1081 cttgaaactt tctcaccttc aacatgcagg ctcgacattg gcaaattttc tggttatctt
     1141 cagctatctg gatgtctaaa cgtataagcg tatgtagtga ggtaatcagg ttatgccgat
     1201 tcgtgtgccg gacgagctac ccgccgtcaa tttcttgcgt gaagaaaacg tctttgtgat
     1261 gacaacttct cgtgcgtctg gtcaggaaat tcgtccactt aaggttctga tccttaacct
     1321 gatgccgaag aagattgaaa ctgaaaatca gtttctgcgc ctgctttcaa actcaccttt
     1381 gcaggtcgat attcagctgt tgcgcatcga ttcccgtgaa tcgcgcaaca cgcccgcaga
     1441 gcatctgaac aacttctact gtaactttga agatattcag gaacagaact ttgacggtct
     1501 gattgtaacc ggtgcgccgc tgggcctggt ggagtttaat gatgtcgctt actggccgca
     1561 gatcaaacag gtgctggagt ggtcgaaaga tcacgtcacc tcgacgctgt ttgtctgctg
     1621 ggcggtacag gccgcgctca atatcctcta cggcattcct aagcaaactc gcaccgacaa
     1681 actctctggc gtttacgagc atcatattct ccatcctcat gcgcttctga cgcgtggctt
     1741 tgatgattca ttcctggcac cacattcgcg ctatgctgac tttccggcag cgttgattcg
     1801 tgattacacc gatctggaaa ttctggcaga gacggaagaa ggggatgcat atctgtttgc
     1861 cagcaaagat aagcgcattg cctttgtgac gggccatccc gaatatgatg cgcaaacgct
     1921 ggcgcaggaa tatttccgcg atgtggaagc cggactaggc ccggaagtac cgtataacta
     1981 tttcccgcac aatgatccgc aaaatacacc gcgagcgagc tggcgtagtc acggtaattt
     2041 gctgtttacc aactggctca actattacgt ctatcagatc acgccatacg atctacggca
     2101 catgaatcca acgctggatt aatcttctgt gatagtcgat cgttaagcga ttcagcacct
     2161 tacctcaggc accttcgggt gcctttttta tttccgaaac gcacctcagg aggtg
//
LOCUS       DAPGWD010000293         2169 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPGWD010000293 DAPGWD010000000
VERSION     DAPGWD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.296"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            156..2141
                     /gene="yjcS"
                     /locus_tag="RHX88_004452"
     CDS             156..2141
                     /gene="yjcS"
                     /locus_tag="RHX88_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDV2233045.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          571 a          575 c          532 g          491 t
ORIGIN      
        1 tccctcttca gcacaaatgc ctaataaaat ggctcgttcc gctcacactt ccacttatta
       61 aagtcgcaat ttcccaatta tgataacgcc agctttaaat gacgtatttt gcatggatta
      121 catatttacg acacaaatga taaatggaga tttatatgaa taactctcgg ttattccgtt
      181 tgagcaggat tgttattgcg ttaactgctg ccagcggcat gatggtaaat accgcttacg
      241 caacagatga agcgaaagcc gccactcaat atacccaaca ggttaatcag aattacgcca
      301 aatcattacc gtttagcgat cgtcaggatt ttgacgacgc tcagcgtgga tttatcgctc
      361 cgctgctgga tgaaggtatt ctgcgcgatg ctaatggcaa accatattat cgcggggaag
      421 attataaatt tgatatcaat gcgcccgcgc ccgaaaccgt taaccctagc ctgtggcgtc
      481 agtcgcagtt aaacggtatt tccggcctgt ttaaagtcac cgacagaatg taccaggttc
      541 gcggtcagga tatctccaac atcaccttta tcgaaggtga taccgggatt attgtcatcg
      601 acccgctggt gacgccaccg agcgcaaaag ccgcccttga cctttacttc caaaatcgcc
      661 cgcaaaaacc gattgttgcg gttatttata cccacagcca taccgaccac tatggcggcg
      721 tgaaaggcat tatctctgaa gctgatgtga aatccggtaa agtgcaggtt atcgcccctg
      781 ctggctttat ggacgaagcc atcagcgaaa acgtactggc gggtaatatt atgagccgcc
      841 gtgcacttta ctcctacggc ctgctgctgg cgcataaccc tcagggtaac atcggcaatg
      901 gtcttggcgt aacgctggca tcgggctacc cgagcatcat cgcaccgaac aaaaccatca
      961 ccaaaaccgg tgagaagatg attatcgacg gcctggagtt tgacttcctg atgaccccag
     1021 gtagcgaagc gccagccgaa atgcacttct atattccggc cctgaaagcc ctgtgtaccg
     1081 ccgagaacgc cacgcatacc ctgcacaact tctacactct gcgcggcgcg aaaacccgcg
     1141 acaccagcaa gtggaccgag tatctgaacg aaacgctgga tatgtggggt aacgacgcgg
     1201 aagtcctgtt tatgccgcac acctggccgg tctggggcaa taagcatatc aatgattata
     1261 ttggtaaata tcgcgatact atcaagtaca ttcacgacca gaccctgcac ctggcgaacc
     1321 agggctacac catgaatgaa atcggcgaca tgattaaact gccgcctgca cttgccaata
     1381 actgggccag ccgtggctat tacggttctg tcagccacaa cgcccgcgcg gtgtataact
     1441 tctatcttgg ctattacgac ggtaacccgg ctaacctgca tccgtatggt caggtggaga
     1501 tgggtaaacg ttacgtgcag gcgctgggcg gttctgcccg tgtcatcaac ctggcgcaag
     1561 aagcgaacaa gcaaggtgat taccgctggt cggcagaact gctgaaacag gtgattgccg
     1621 ccaacccggg tgaccaggtc gcgaagaatc tgcaagcgaa taactttgaa cagctgggct
     1681 atcaggccga gtccgccacc tggcgcggtt tctacctgac cggcgcgaaa gagctgcgcg
     1741 aaggggtgca taagttcagc cacggcacca ccggttcccc ggacaccatt cgcgggatgt
     1801 cggtcgaaat gctgttcgac tttatgtccg ttcgcctcga tagcgcgaaa gccgcgggta
     1861 aaaatatcag cctgaacttc aatatgagca atggcgataa cctcaacctg acgctgaacg
     1921 atagcgtgct taactaccgt aaaacactgc aatcccaagc tgacgcctct ttctacatca
     1981 gccgtgaaga tctgcacgcc gtgctgaccg ggcaagccaa aatggcggat ctggtaaaag
     2041 cgaagaaagc caaaattatt ggcaatggcg cgaaactgga agaaattatc gcctgtctgg
     2101 ataatttcga tttgtgggtg aatatcgtaa ccccaaatta atgtcactcg cttttggggg
     2161 tgctgaagc
//
LOCUS       DAPGWD010000294         2148 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPGWD010000294 DAPGWD010000000
VERSION     DAPGWD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.297"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..686)
                     /locus_tag="RHX88_004453"
     CDS             complement(<1..686)
                     /locus_tag="RHX88_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDV2233046.1"
                     /translation="MLSLPGSFLTPPATLSVAIDYPAALALRQMALVQGELPKYLLAP
                     EISALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQRTE
                     KATRTAGRVPAGSQVHRLVPLSDTQYVSQLQMMVATLKIPLERRNKRTGRMEKARIWE
                     ITDRTVRTWLNEAVEAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMGHK
                     SISSTDVYTKV"
     gene            complement(698..1387)
                     /locus_tag="RHX88_004454"
     CDS             complement(698..1387)
                     /locus_tag="RHX88_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233047.1"
                     /translation="MADWFIATEGVKVVKDSASLWPQIITAVLSAGTAFGGVWYGQWR
                     ITQREEEAATAKLVSERLFIATELVFLLERFAQRCAPAATATVEHDQDGRGEAEFLLP
                     EIDYSPVSGNWRSLPSGLMYRLSELSVLSYEAVVCVDNVFVEDTPYGGAGEYSLHKNA
                     AMLGVKALRLSRELRMLCGLPLHGLSDTLSPTRLVLLKARGKTLQKEYEMVKKSKKTE
                     QEIEDFIKGTS"
     gene            complement(1380..1664)
                     /locus_tag="RHX88_004455"
     CDS             complement(1380..1664)
                     /locus_tag="RHX88_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233048.1"
                     /translation="MAGSPMQKFLRRALLMKIAQQLLEAVLQFMNASAMTQVLTLIFL
                     ALIIQVLKTLITAWIVRKPSSGEMVLDRKPDSAGRKDHCRQLSQQRKKHG"
BASE COUNT          526 a          570 c          503 g          549 t
ORIGIN      
        1 accttcgtgt acacatctgt cgaactaatc gacttgtgcc ccatcaggct ctgcagcacc
       61 ttcagcggaa taccagcata cagcatatgc atcgcgtagg aatggcggaa agtatgtggg
      121 gtaaccggaa cagagaacgt cacgccgtcg gccgcagcgg cctctacagc ttcattcagc
      181 caggtgcgaa ctgtgcggtc ggtgatttcc cagatgcgcg ccttctccat tcttccggtt
      241 cgtttgtttc tgcgctccag tgggattttc agcgtggcca ccatcatctg cagttggctg
      301 acgtattgtg tatcggacag gggaaccagg cgatgcacct gactgccagc agggacacgg
      361 cctgccgttc tggtcgcttt ttcagtccgt tgtttgagcg tggccagctg tacgaacgga
      421 tatggcggcg ccagtgaaaa atctccccgc gtcagcgcca gcgcttcgtt tatacgtgcg
      481 cccgtgttcc acagggtggc cagcaacatt ttgcggtgca gatccggtac atagtggagc
      541 agggcgctga tctccggcgc caacagatat tttggtaatt cgccctgcac aagtgccatc
      601 tgacgtagtg ccagcgcagc cgggtaatca atggcaacag ataatgttgc cggaggcgtt
      661 aaaaaagaac caggcaggga aagcaattgt gggccactca tgatgtcccc tttatgaaat
      721 cctcaatctc ttgctcggtc tttttagact tctttaccat ttcgtactcc ttttggagtg
      781 ttttacctct ggctttcaac aataccaacc tcgttggcga tagtgtatcg gaaaggccgt
      841 gcagagggag gccacataac attctcagtt ctcgtgataa ccgcagtgct tttactccca
      901 acatagctgc gtttttatgc aaactatatt cacccgctcc accataagga gtatcttcaa
      961 caaacacatt atctacacac acgacagctt cgtagctgag aacagagagt tcgctgagcc
     1021 tatacatcag gccagaagga agggagcgcc agttaccaga taccggggaa taatcgattt
     1081 ccggtaataa gaactcagct tctcctctgc catcctgatc gtgctccaca gttgctgttg
     1141 ctgctggagc acaacgctgc gcaaacctct ccagcagaaa aaccagctcc gtggcaataa
     1201 agagacgttc gctgaccagc ttcgctgttg cagcttcctc ctctcgctgt gttatccgcc
     1261 actgcccgta ccacacacca ccaaatgcag taccggcaga caaaaccgcc gtgatgatct
     1321 gcggccacag gctggcacta tctttcacca ccttcacacc ttcagttgca ataaaccagt
     1381 cagccatgtt ttttcctttg ttgagacagc tgcctgcagt gatccttacg cccggcagag
     1441 tcaggtttcc ggtctaaaac catctctcct gaagatggtt ttcttactat ccacgccgta
     1501 attaatgttt tcagtacctg tattatcaga gccaggaata tcaacgtcag cacctgagtc
     1561 attgcagatg cattcataaa ttgcagaacg gcttccagta attgctgagc aattttcatt
     1621 aaaagagctc tccttaaaaa tttttgcatc gggcttcctg ccattggttg tggatgccga
     1681 aattctaaaa gaagtcactt gtcagccttg cgggcaaaaa aaatttttac tggtcagtaa
     1741 aacgtggtgg tctgaaaatc ctcactcaac tgataccgga agcaacagtc gctcagagtt
     1801 attacgcatg gcagcacaac actctgtcag catgaattat tttcccctaa ataaatcatc
     1861 acctgtcaga taataagcag gcatataacc acgccattaa attatcggat gcccatttca
     1921 ggcagatccg atttttctgt tattgacggg tttccggaag tgtaccacgc tgaggaaatc
     1981 atcatatcgc tgttttccgc ccctgcgcga cgcatcacag gtcagggaga tgacacaaca
     2041 tatagtgtca tcagtagctg aatccggcac cacatattgt gcttacagga ggtggcgttg
     2101 tgtgggggga gaggaagatc ctgaactaac gcttttcgga tcttcgtg
//
LOCUS       DAPGWD010000295         2142 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPGWD010000295 DAPGWD010000000
VERSION     DAPGWD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.298"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            745..1035
                     /locus_tag="RHX88_004456"
     CDS             745..1035
                     /locus_tag="RHX88_004456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233049.1"
                     /translation="MIRKIAEGHISRVSGKVMSAVIRECEDFPPTTVKDIIELLEFYD
                     ETASRVLAGHHRQIPTNNPLLKPWVFGVRFYAYNHFNAKLVTICDLPPTCLF"
     gene            1215..1523
                     /gene="istB"
                     /locus_tag="RHX88_004457"
                     /pseudo
     CDS             1215..1523
                     /gene="istB"
                     /locus_tag="RHX88_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
     gene            complement(1686..1989)
                     /locus_tag="RHX88_004458"
                     /pseudo
     CDS             complement(1686..1989)
                     /locus_tag="RHX88_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610815.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          589 a          545 c          468 g          540 t
ORIGIN      
        1 ataaccttaa aaatagcttt tcacgaacac cgaaaggatg acgatgggaa gtggattaat
       61 cttaaaataa tacttctaaa ttacaaaggt taaaagcaaa tcacaacctt cccgaacctc
      121 cgcttttcgc ttgctacggt ccctgccgag catgaatctc gtctatgccc aaaagcggac
      181 attgataatt cagaaaccat ggcagaagtg tggtatcagc ccctcacctg atgcctgcca
      241 gcatcgcacc tcgcttcatg tctaacctct ctgttagccc ggcagggtca atggctctgt
      301 tgacaatggt gctactttcc aatacgttaa gcacaaggtt agcgcgaccc ggcagctcac
      361 ggccaaagcg gaatgcagcg cagacttatc aaatcttttt tggttaagtg tctttgattc
      421 ttatgattcg tgagcacata ggacagataa gagacttaaa ccatgaaaca aatccccaca
      481 tgcgctgaag caaaagccca cgccaaatac ctttcacggt cgctcaatat caatctaagc
      541 tacgcccggg atgcagtggc attacgttat aactaccata actggtcgga actcagcaca
      601 gtatttggtc agcttagctg ataagtacat gtcgtgctat ggccttgcca gtcatgaaga
      661 gaagcgtgtt tttagccagc ttctggcacc atatatcgca gaattgcaaa atgtcattca
      721 tcctgaccgc catgtacgga aagcttgata cgtaagattg ctgaaggcca tatttcccgg
      781 gtatctggta aagtcatgtc ggctgtaata cgtgaatgcg aggattttcc tcccacgaca
      841 gttaaagaca tcattgaact gcttgaattt tatgatgaaa cggccagccg ggtattagca
      901 ggacaccacc ggcagatccc gaccaataat cccttgctga aaccctgggt atttggtgtg
      961 cgcttttatg cctataacca ctttaatgcc aagctggtca ccatctgtga tcttccgcca
     1021 acatgcctct tctgaactct cccgacgtca acaaaacccc ctctaatccc ttatatatca
     1081 ggatttcttt gtatccagag gccaaccgcc ttcaccccgt atcaagttac ttttgggggt
     1141 ataatttact cttgaaatat cagcacccca taatgaaaaa aatgtgatac ataacaacgg
     1201 attttaagta agcgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa
     1261 taggctatct gccgttcagt caggaagaag caaagctgtt cttccaggtc atcgctaaac
     1321 gttacgaaaa aagcgcaata atcctaacat ccaatctgcc gttcgggcag tgggatcaaa
     1381 cgttcgccgg tgatgcagcc ctgacctcag cgatgctgga ccgtatctta caccactcac
     1441 atgtcgttca gataaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta
     1501 tagcagaagc taatcctgag taacacggtg gatcaatatt gggccgttgg tggagatata
     1561 agtggatcac ttttcatccg tcgttgacac atgcatcaca acagctcctc ataggcactc
     1621 agtggtcgat gttccacctg actgatctgc tgccagagcg gggcgtgagg ttccggtact
     1681 atctgctagc cagacgacac tgagcagagg cgatgtgagg ccaccagttt ctcattactg
     1741 tagatccgca actcatcgtt cagcgatatt cgtatcgaga ctggctgacc acacagcatt
     1801 tcgggaacgc tgtactgatt acctccaact tcgttatagc gggtcccagg acacatggcg
     1861 gatgtcgaag taactggtat cgaagtctgt atccggtaac ggctgcagat gttcctgctc
     1921 cagcgcgaag cgctgttccg gcgtttcttt gaactgacga agctcccatt tgtcagccac
     1981 atcggctagc gccagctaac ctatgcggta ctggccttgg tgctaacccg cgttaaagct
     2041 accacgttga tcagagcctc tctcccggaa agtcatactg tcttacatat taatcactca
     2101 aaatagagta aataaaatca tacaactaga gagtgattat aa
//
LOCUS       DAPGWD010000296         2127 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPGWD010000296 DAPGWD010000000
VERSION     DAPGWD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.299"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            123..1256
                     /gene="ompN"
                     /locus_tag="RHX88_004459"
     CDS             123..1256
                     /gene="ompN"
                     /locus_tag="RHX88_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDV2233050.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1397..1831
                     /gene="uspF"
                     /locus_tag="RHX88_004460"
     CDS             1397..1831
                     /gene="uspF"
                     /locus_tag="RHX88_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDV2233051.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT          594 a          479 c          470 g          584 t
ORIGIN      
        1 ttaaggtttt acttaaggcg taacaaatga tatttatcgg ctaactgaac ttctccttta
       61 ggatgttttc actccccgaa atggggataa aaggcaaata aaataactaa aggatttatt
      121 caatgaaaag caaagtactg gcacttttaa ttcctgccct gctcgccgca ggtgctgcac
      181 atgcagccga agtttataat aaagacggca acaaattaga tctgtatggc aaagttgatg
      241 gcctgcatta tttttctgat aattcggcga aagatggcga ccagagctat gcgcgtctgg
      301 gttttaaagg cgaaacccaa attaacgatc aactcactgg ctacggtcaa tgggaataca
      361 atattcaggc aaacaacact gaatcttcaa aaaaccagtc atggacccgt ctggcatttg
      421 ccgggctgaa atttgcagat tacggttctt tcgattacgg acgtaattat ggcgtaatgt
      481 acgacatcga aggctggacc gatatgctgc ctgaatttgg cggtgactct tataccaatg
      541 cagacaactt tatgactggt cgagccaatg gcgtcgcgac ttatcgtaat actgatttct
      601 tcggtctggt aaatggtctg aacttcgcgg tgcagtatca aggtaacaac gaaggtgcca
      661 gtaatggtca ggaaggcacc aacaacggac gtgatgttcg ccatgaaaac ggtgacggct
      721 ggggtctttc cacaacatat gatttaggca tgggctttag cgctggtgcg gcatacacct
      781 cttctgaccg caccaatgat caggttaacc atactgcggc gggtggtgat aaagcagacg
      841 cgtggactgc tgggctaaaa tacgatgcta acaatattta cctggcaacc atgtattcag
      901 aaacgcgtaa tatgaccccg tttggcgaca gcgattatgc tgttgcaaac aaaacccaga
      961 actttgaagt cactgcacag taccagtttg attttggtct gcgtccggca gtctctttcc
     1021 tgatgtctaa aggccgtgac ctgcacgctg ccggtggtgc agacaacccg gcaggtgttg
     1081 atgataaaga tctggttaaa tacgccgatg ttggcgcgac ttactatttc aataaaaaca
     1141 tgtccactta tgttgactat aaaatcaacc tgttggatga agatgacagt ttctacgctg
     1201 ccaatggcat ctctaccgat gatattgtcg ctttaggtct ggtttatcag ttctaaatcc
     1261 tcctgcccgc tgttatggcg ggctttttct gcttattctt cctcctttga tctaaattaa
     1321 aaatgtgaac tccgtcatta cacaaaaagt gtcatctggc gttacacttt atgcggatac
     1381 taaaacagga ggttttatga acagaacgat tcttgtccct atcgatattt ccgattcaga
     1441 attaactcaa cgcgtgatta gccacgttga ggcagaggca aagattgatg atgcagaggt
     1501 tcatttcctg acggtaatac cttcactgcc ctactatgcc tctctgggtt tagcgtattc
     1561 cgcagaatta ccggcaatgg atgacctgaa agcggaagcc aaatcgcaac tggaagagat
     1621 cattaaaaaa tttaaactgc caaccgacag agtgcatgtc catgttgagg aaggctcgcc
     1681 caaagaccgc attctggaaa tggcgaagaa gatccccgct catatgatca tcattgcttc
     1741 ccatcgaccg gatatcacca cttatctgct cggttccaac gccgcagctg tagtgcgtca
     1801 cgcagagtgc tccgtgctgg ttgtgcgctg acactaacgc ccgcacattg ctgcgggctt
     1861 tttgattcat ttcgcaaatg tgctgacatt ttcccctcta atccgtacca tacacgccac
     1921 agtttttata tcagatttct tatgctgggc gttccggcat gacgcatact cttctgatgc
     1981 catataacga attgagtcgc ttttaaatgt cgcaaaatca agaaattagt aagaaagaac
     2041 aatacaacct gaacaagtaa gggcaaaaat cacaactctc tgactcataa gtattttact
     2101 tatttttcaa tgtgttaaat atcatca
//
LOCUS       DAPGWD010000297         2070 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPGWD010000297 DAPGWD010000000
VERSION     DAPGWD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.300"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..244)
                     /locus_tag="RHX88_004461"
     CDS             complement(<1..244)
                     /locus_tag="RHX88_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDV2233052.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMF"
     gene            complement(287..523)
                     /locus_tag="RHX88_004462"
     CDS             complement(287..523)
                     /locus_tag="RHX88_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDV2233053.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            837..2048
                     /gene="bluF"
                     /locus_tag="RHX88_004463"
     CDS             837..2048
                     /gene="bluF"
                     /locus_tag="RHX88_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDV2233054.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          601 a          432 c          410 g          627 t
ORIGIN      
        1 tgaacatact tttattgtca gctgtcgctg ttcttcggtt tggccctcct gaatcactat
       61 tgctaaaatc acgaattatg ctgtcaatta aatcgcgtcg gaaagcatct tcctcagccg
      121 atttcattac gagtaattgc ttaataagag tctgcaatga aaaatttatc ttcatgcgca
      181 gtatctggat caccgcctgg ccagtaattt catattttat acgttcatta tcagatgtct
      241 tcataacgac ttcatagcta acctgttaaa taatcaggct gttgcattat tcaaaaagca
      301 acccaattag tgcattataa tgtttctgtt cctcttcccc agtcgcgtgt tccagtcggc
      361 aaagaagttt ggcacaaagc gactttcgac tcagattacg tccatctttc aaaatctcag
      421 tcacaatttc cccgagagtt tcctgctgag taggcaagtt agcttttgca aagtaacgag
      481 tgattgctcc cgcagaatct aaagtcactg aattttgatg catgctacgc ctctgttaaa
      541 aatgttaatc aatgttaagt gaggtaacaa tagcaaactt gtacgaaata tgtgtacaga
      601 atgataaatt aattttttgc aattagtgct aatgcatata aaacaatgtg ttatgataaa
      661 acatattgca tcatttaact gacaatctat acaagatttt tcgccaacta gtgttcaact
      721 ttttccattt tttatcaatg taaagaaacg gttatgtttt ggtattaaaa gaaagacaca
      781 agaagatatg tctgttaccg tcttactctc gcctcaccca ttaccctgga ttgttaatgc
      841 ttaccaccct tatttatcgt agccatatac gtgacgacga acctgtcaaa aaaatcgaag
      901 aaatggtttc gatagcaaat cgcaggaaca tgcagtctga cgtaacaggg atcttactgt
      961 ttaatggttc tcattttttc cagcttctgg aaggtccgga agaacaggtt aaaatgatat
     1021 atcgggctat atgccaggat ccacggcact ataatattgt tgagctgctg tgcgattacg
     1081 cgcctgctcg ccgttttggc aaagcgggaa tggaattatt tgatttgcgc ctgcacgagc
     1141 gagatgacgt tttacaggcc gtattcgaca aaggcacatc aaaatttcag ctaacttatg
     1201 atgacagagc gctacaattt tttcgtactt ttgtccttgc aaccgaacaa tcaacctatt
     1261 tcgagatccc tgccgaagac tcctggcttt ttatcgctga cggatctgat aaagaacttg
     1321 attcctgtgc cctttcacca actataaacg accactttgc ctttcatcct attgtcgatc
     1381 ccttatcgcg gcggataatc gcttttgaag ccattgtgca aaaaaatgaa gatagcccat
     1441 cagccatagc ggttgggcag cgtaaagacg gggaaatcta cacagcggat ctcaaaagta
     1501 aggcgcttgc attcacgatg gcacacgcac ttgagctcgg tgataaaatg atttcaatca
     1561 atctattacc tatgaccctg gttaacgaac ctgacgcagt ctctttttta cttaatgaaa
     1621 taaaggccaa tgctctggtg cctgaacaaa tcatcgttga atttactgaa agtgaagtca
     1681 tatctcggtt tgatgagttt gccgaagcga ttaaatcgct aaaggctgct ggtatcagtg
     1741 tagcaattga tcattttggc gcaggttttg ctggtttgtt actcctgtca cgcttccagc
     1801 ctgacagaat taaaatcagt caggaattga ttaccaatgt tcataaaagt gggccacggc
     1861 aggcaattat tcaggcgatc ataaaatgct gtacatcact tgaaattcaa gtcagtgcta
     1921 tgggcgtggc aacaccagaa gagtggatgt ggcttgaatc cgcaggaatt gagatgtttc
     1981 agggagatct gtttgctaaa gctaaattga atggtatccc ttccatagcg tggccagaga
     2041 aaaaataatt ttcagcacat tctttcacat
//
LOCUS       DAPGWD010000298         2048 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPGWD010000298 DAPGWD010000000
VERSION     DAPGWD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.301"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            457..984
                     /locus_tag="RHX88_004464"
     CDS             457..984
                     /locus_tag="RHX88_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV2233055.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGVKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            1042..1275
                     /locus_tag="RHX88_004465"
     CDS             1042..1275
                     /locus_tag="RHX88_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV2233056.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGALVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            1334..>2048
                     /locus_tag="RHX88_004466"
     CDS             1334..>2048
                     /locus_tag="RHX88_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDV2233057.1"
                     /translation="MPVTKCEPETTRKASRKSAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESE"
BASE COUNT          451 a          587 c          634 g          376 t
ORIGIN      
        1 cgctacggtc cgatgcgtga aaacccgggc ttgcagcgac cgttccggtc gcgccgcccc
       61 tgtcgcgccg tgacctgcgc tccctgcaca cctgacggtg tgtgaggaaa ctgcgcctga
      121 tggcgcagca gtcttccctc ctgccgccac ctctgcggcc tgtttcccgc tgtggacttt
      181 ccggggggca gacgtaaccc cctcccgttc catctgtcca gggtgagatg caccggccac
      241 aaaattttta gccatgaacc cggcaaaaaa ttttgcgtcc gcccccggac agctgtcccg
      301 tgaggcggta aacgggcccc catcggaaag gtccactgcg gggagaaacc cctgtgaagt
      361 ggacggcagg aggagctttc gctcctctga aggggcacat gaacgtcccg tcgccggtat
      421 ccggtttatc tcacatcttt cagtcaggag gttcttatga gtgcacgtgg tatcaacaag
      481 gtcatcctcg tcgggcgtct gggcaatgat ccggaagtcc gttacatccc caacgggggc
      541 gcagtggcaa acctgcaggt ggctacgtca gaaagctggc gcgacaaaca gacgggggag
      601 atgcgggagc agacggaatg gcaccgcgtg gtgctgttcg gcaagctcgc ggaagtggct
      661 ggtgaatatc tgcgcaaggg cgcgcaggtc tacatcgagg ggcaactccg tacccgtagc
      721 tgggaagata acggtatcac ccgttacgtc actgaaattc ttgttaagac cacgggcacc
      781 atgcagatgc tggggagtgc accacagcag aacgctcagg cgcaaccgaa gcctcagcag
      841 aatgggcagc cacagagtgc tgacgcgacg aaaaaaggtg gcgtgaaaac gaaaggccgt
      901 ggacgtaagg ccgcgcagcc cgagcctcag ccgcaaccgc cggaggggga ggattacggg
      961 ttttcagacg atatcccgtt ctgactggcc tgactgtgat aaccgccccg ccctgcgcgg
     1021 ggcatcaccg gagatatgag gatgagcgaa tatttcagaa tacttcaggg gctgcctgac
     1081 ggctccttta cccgcgaaca ggcggaagcc gttgccgcac agtaccggaa cgtctttatc
     1141 gaggatgacc acggggaaca ttttcgcctg gttgtccgca ataacggtgc cctggtctgg
     1201 cgtacctgga attttgagga cggtgccggg tactggatga atcatgccat cagggatttc
     1261 gggattctta agtaagagag gtgccggacg cgcgaacgtc cggcaggaca taagcaatta
     1321 taaggggatg attatgcctg taacgaagtg tgaaccagaa accacccgca aagcaagccg
     1381 taaatctgca aaaacgcagg aaacagtcct gtctgccctg ctggcgcaga cggaggaagt
     1441 gagcgtgccg ctggcctcac tgattaagtc accgctgaat gtgcgcacgg tgccgtattc
     1501 tgcggagtcc gtcagcgaac tggctgagtc cattaagggc gttggcctgc tgcagaatct
     1561 ggtcgtgcat gccctgccag gtgaccgtta cggtgtcgcc gcaggcggtc gccgactggc
     1621 agcactcaac atgctggcag agcgcaacat ccttccggct gactggcctg tccgcgtgaa
     1681 aattattccg caggaactgg cgacggctgc atcgatgacc gagaacggtc atcgtcggga
     1741 tatgcaccca gccgaacaga ttgccggatt ccgcgcaatg gcgcaggaag gcaaaacacc
     1801 tgcgcagact ggcgacctgc tgggctattc accccgccac gtccagcgaa tgctgaaact
     1861 ggcagacctt gcacccgtca tccttgatgc gctggcagaa gaccgcatca ccaccgaaca
     1921 ctgtcaggcg ctggcgctgg agaacgacac cgcgcgtcag gtgcaggtgt ttgaagccgc
     1981 ctgccagtca ggatggggcg gtaaaccgga tgtgcgggtt atccgcaacc tgattaccga
     2041 aagtgaag
//
LOCUS       DAPGWD010000299         2033 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPGWD010000299 DAPGWD010000000
VERSION     DAPGWD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.302"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     repeat_region   122..1919
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=122..150
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
BASE COUNT          445 a          558 c          581 g          449 t
ORIGIN      
        1 tttttaatca atggattaag tactctttaa cataatggat gtgttgtttg tgtgatacta
       61 taaagttggt agattgtgac tggcttaaaa aatcattaat taataatagg ttatgtttag
      121 agtgttcccc gcgccagcgg ggataaaccg gatacgcttc atcgcccgac gtggggagca
      181 tagtgttccc cgcgccagcg gggataaacc gatattgtca gaaactggct ggagcatggt
      241 gacgtgttcc ccgcgccagc ggggataaac cggtggctga acttccgatg cgatgaatgt
      301 tattgtgttc cccgcgccag cggggataaa ccggcgaatg ccccatctgt aaacgccggg
      361 gaaaagtgtt ccccgcgcca gcggggataa accgggattt tctttggtgg ggaatgcccg
      421 ccgtgtgtgt tccccgcgcc agcggggata aaccgttgat tacctcgctg ttgctgcgct
      481 actggtggtg ttccccgcgc cagcggggat aaaccggcgt ttccatgccg ttgataaaca
      541 tcccgcccgt gttccccgcg ccagcgggga taaaccgggt tgccatcgct gacgcaacgt
      601 cattaaggcg tgttccccgc gccagcgggg ataaaccggg aataattacc gcgctgatta
      661 aattaattaa gtgttccccg cgccagcggg gataaaccgg cggcagcgcc cgccgggtac
      721 gacggcagca cgtgttcccc gcgccagcgg ggataaaccg cgcttttgct cagtagtgag
      781 tgtgatttca tcgtgttccc cgcgccagcg gggataaacc gcaggtacag ccgtttcggt
      841 gttttgtttt tgtgtgttcc ccgcgccagc ggggataaac cggcgtcgct ggaagaacaa
      901 aaccgcctga tcgagtgttc cccgcgccag cggggataaa ccggcaccac gcgtaccccg
      961 atgtttgttt tgccagtgtt ccccgcgcca gcggggataa accgatccgg ctatatcttt
     1021 gagcattaca gaaatagtgt tccccgcgcc agcggggata aaccgctatt gctttcgtac
     1081 agattttcag tggtgctgtg ttccccgcgc cagcggggat aaaccgaaaa ttctgtgttt
     1141 cgaccattac ttcggtaagt gttccccgcg ccagcgggga taaaccgatt cttgatcacg
     1201 cttttaccga agtaatggtg tgttccccgc gccagcgggg ataaaccggg ggtagaatta
     1261 ttcttcgtga gcgatttatc gtgttccccg cgccagcggg gataaaccgc ggcgttccgt
     1321 gcggcaattg gaatcacacc agtgttcccc gcgccagcgg ggataaaccg cgttctgaat
     1381 ccgatattct tcagcacctt cagtgttccc cgcgccagcg gggataaacc gagcgtcaat
     1441 cagcgcgtct atcgcgtcac tttgtgttcc ccgcgccagc ggggataaac cgatttgggg
     1501 gtatgagagc gccgagccgt tcgggtgttc cccgcgccag cggggataaa ccggctccct
     1561 gtcagttgta atcgataacg ttgatgtgtt ccccgcgcca gcggggataa accgatgtag
     1621 gggcaatcga acgattctct gccgacgtgt tccccgcgcc agcggggata aaccgccgag
     1681 cccgattatc ggcatgagcg atgcggagtg ttccccgcgc cagcggggat aaaccgtcga
     1741 agaagaaagg gaaataatgc gaggaacggt gttccccgcg ccagcgggga taaaccgtat
     1801 tacgcgccag caatgctgac agcggcaaag tgttccccgc gccagcgggg ataaaccgcg
     1861 cgagagccag caaaacgcca gggcacaaaa gagttccccg cgccagcggg gataaaccac
     1921 catataaccc gttatctctt tctcaagttt ttatattagc agtacttgta ataagcaaca
     1981 tatccacgta acccctcatg ttcaaaatag ttctccatgc cagagaagtt cac
//
LOCUS       DAPGWD010000300         2026 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPGWD010000300 DAPGWD010000000
VERSION     DAPGWD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.303"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1341)
                     /locus_tag="RHX88_004467"
     CDS             complement(<1..1341)
                     /locus_tag="RHX88_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HDV2233058.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGG"
     gene            complement(1338..>2026)
                     /locus_tag="RHX88_004468"
     CDS             complement(1338..>2026)
                     /locus_tag="RHX88_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV2233059.1"
                     /translation="ILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGIDARD
                     DLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTAECC
                     GEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQYFAGYTF
                     GRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQPLAYYSAC
                     PETLKDMSDL"
BASE COUNT          552 a          499 c          463 g          512 t
ORIGIN      
        1 cccaccatcg tcaacgggca ttggctgcgg ttgcggctcc ggttcaacag tttgcttctc
       61 ggtaaaattc gccgcctgaa ttaataatcg acgaataatt aatgcttcca gtggacaacg
      121 atcttctgca tcaaggcggc tcacaatcgg cattaatgat tcttcggttg gttggttcgc
      181 ctgcactaat tgtttggcaa ctaaatataa tggccataag ggatgttctg tatcacgcgt
      241 gagtgagatc cgcggcttac gtcctttgtg caaaaccatc tcgaccagtg aacgccccag
      301 agcagagagt ttttcatcgc tgagtatttc gtataaatga aaacgcgtac gggcgctgtc
      361 gccggggata taaagtagcg gttttccctg atcgttaacc agttcacgag cttcaatcat
      421 catcaccact aacgggtaat aatcgacccg atgctgacgg caccattgca atattaaagc
      481 atccgcctgg gtggagggat actcgcgcca gtagtcacaa agttctgcca gcaagctttc
      541 gccttctcca caatagacgc gttgtgcgta ttcataataa cgcgcatctt cattatcaga
      601 ttcagcttcc cgccacgacc gggcgtaatc caggagttcg ggaattccca ggcgacctgc
      661 gctataccag cggagtaaat ttctgtgcag ttttgcatca tcggggataa tcactgcgcg
      721 atgttgcgct aaatacaatg tcagccattc tggatgcgat gaccaaatcc gagcgaacat
      781 ttggtaaaaa tcgatggtgt cattttgcac ggcaacgggc agatgcagaa tggtctggaa
      841 gttaaaaaca ttccccttcg caatggcata taaaaaggat tccagttctt cttcgtctat
      901 ttccccgacg ttttcctcct gcaaccaccg caatctttct gctaataaca ccacgcactc
      961 gaaggataaa tgggcagtgt tcatggcgat tgtgcacagc gaccagcgta attcatcaat
     1021 ctcttccatc gagcaataat ttaattgctg aatgaatcgc tgccaggcgg agggcagaaa
     1081 acgttcgcta tcagaggcaa gtaacgcacg aaaggcgagc agaatttcat gttctgctac
     1141 ctcatattct tctggttgcc aaacagattt agcaggcgac tgcgcaatcc gtagcgcctc
     1201 ctcatacgcc tgacgaagtt gtttaaaacc ctgcggatcg gtttctggat gaaaggacgg
     1261 taacagcgcc agataagcgc ggcggataat atcgacgtca gtcgtttcct ctatttcgag
     1321 aatcttccag caatttttca taggtcgctc atatccttaa gcgtttccgg gcatgcagaa
     1381 tagtaagcca atggttgcca ggacaaggta gggtaatacg catcatcact ggcaaataat
     1441 tgataaaaca tatcattgcc ggaatcgtcg tattctttac gcgctaagaa ttcgcgtaat
     1501 aactgcagat gatcatcttc gggagactgc caatattgcc gaccaaaggt ataaccggca
     1561 aagtattgcc gccagttacg ataataacgt agagcccgta gatgaatgcg cgactggatc
     1621 catagacttt cttcttcgct caaccagtta ttaagtacgc ccattcggct taaaaaaccc
     1681 atccggacat aatcccaggc tttaattcct ccctcaccac agcattcggc ggtgctggcg
     1741 acaaattgcg cataagcctg accttgctca tttagttcag caagatagtc acgccattca
     1801 catggcgaat aacgaaacca gcggtgataa aaccctgcca accgagcggc atgaccgtta
     1861 tcgatcattc ggtgaattgt gcgcagcagg tcatcacggg catcaattcc ccaggaggaa
     1921 gaaaggtcaa aaaagcatgt acctaaatac attttgggtg atgaatgcga aggtaaacag
     1981 cattcattgc gtatttccat cggagtggaa agtgcaaaca atattt
//
LOCUS       DAPGWD010000301         2000 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPGWD010000301 DAPGWD010000000
VERSION     DAPGWD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.304"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            91..342
                     /locus_tag="RHX88_004469"
     CDS             91..342
                     /locus_tag="RHX88_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDV2233060.1"
                     /translation="MKTTNSILRVIASVIAAGQNQISITELVSLSGISRQSVKRAIQI
                     LEQTGQITVTRTTTNGKRGANTYYLPDQDLGGGCRSTLS"
     gene            complement(738..893)
                     /locus_tag="RHX88_004470"
                     /pseudo
     CDS             complement(738..893)
                     /locus_tag="RHX88_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219394.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1234..>2000)
                     /locus_tag="RHX88_004471"
     CDS             complement(1234..>2000)
                     /locus_tag="RHX88_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2233061.1"
                     /translation="PPGQRLFQLVICIFYFSIPFSLMIYGETYVSSGLASLIFANMPV
                     AVLIASSLFLKEKTNSMQIMGLTIAIVALAGILLEESKTSAESHWQGIIALVSAVIIH
                     AIIYTQCKKRSCTVSVVTFNALPCFLAGLILSATGWFFERPEVSTFSVYSILATLYLG
                     AFAGVFGILCYFALQQKASAFQASLVFLIFPLIAVSLESCIYGYGISTQSMLFIIPLV
                     IGLFLTLFLRRVPVTNGHRDSSSQKANEIAEHAENS"
BASE COUNT          618 a          466 c          425 g          491 t
ORIGIN      
        1 tttctttgaa ctacaattcg atgaccttcg gacgacatcc agacagccgc aagaaaaaac
       61 atggcctagc agagataagg ggcgctgaca ttgaaaacga caaattcaat cctacgtgta
      121 atcgccagcg tcattgctgc cggacaaaat caaatctcaa tcactgaact ggtaagcctc
      181 tccggtatta gtcgacagag tgttaagcgg gcaattcaga tactggaaca gacagggcaa
      241 atcaccgtta ccagaacaac caccaacggc aagcgcggag ccaacaccta ctacctgcca
      301 gaccaggatt tgggaggcgg ttgccgttca acattatctt aaccacggct gctgcctgga
      361 gtttacgggg tctgaagccc aaccgtacga aaacgtacta taagggcttc cccggcatgt
      421 caacgccagt ttttatgttt ttgcctgctt gacgtatagg aatttgaaaa accaggcgtc
      481 ctcattaaaa ataaagggaa aagactgcgg tactataaac ggtcataatg aatcgtcatc
      541 gattcgaccc ctgatgaaag acgctcgtga ggttcttgtt cgctaagcgg atatgtatat
      601 cctgtacgtt ttgcagatat tcctcacggg gtataaccct ttatcatcaa cgagttgcag
      661 gtcaaatggt catactgttg cagcgtgctt tagctggctg aatacagtct gaaatcataa
      721 ccgtaatgca atgatgctca gtacagcctt gcatttttgg cggcgatggc cacaactgcg
      781 cgccagtatc ctctgcgctc gaccagtgaa cagtcccaac gactgaatga gtcagtcctt
      841 ttctcagcac cagtcagtac tgaacgagcc ccctgtacca gaagcgttcg cagcttgccg
      901 aaataacctt gcctggttgt gagcttcgga acatgcctcc atcccgataa cacagggctg
      961 caaatcagca atcagctcag gaagcccaat tcgtgatatt ctggtgttaa ccaaagccgt
     1021 ttaccatcct ggtcaatacc gtgaacagta agcatatttt tagctagatc gacacgatag
     1081 tagcaatagt cataacgaat ccatccggac tatgcttacc ctcataattg cacaagagtt
     1141 aacctggtgc atattcaggg gaagcccctt ccatccgtta agtgtaatgg cctacattaa
     1201 caactgaaaa cgaaaagtct tttcttcaag ttttcaggaa ttctccgcat gttcagcaat
     1261 ttcattagct ttttgtgatg aactatctct atgtccgttg gttacaggca ctcgacgtaa
     1321 gaacagagtc agaaatagcc ctataactag cggtataata aacagcattg attgagttga
     1381 aattccatat ccatatatac agctttcaag gcttaccgca atcaatggaa aaataaggaa
     1441 aacaagagaa gcctgaaagg cactggcctt ttgctgaagg gcaaagtagc acaatattcc
     1501 aaaaactccg gcaaaagcac cgagatacaa ggtggctaat attgagtaca cagagaaggt
     1561 cgatacctcg ggtctttcga agaaccatcc tgtcgcagaa agtatcaatc ctgccagaaa
     1621 gcacgggagc gcattaaacg ttaccacaga gacagtacaa cttcttttct tacattgagt
     1681 atatattatg gcatgaatga ttaccgcaga aacaagagca ataatcccct gccagtgact
     1741 ctctgcactt gttttcgatt cctcaagaag gatgcccgcc agtgcaacta ttgcgatagt
     1801 taaacccata atttgcattg agttcgtttt ttccttcaaa aacaaagatg aagctatcaa
     1861 aacagctaca ggcatattcg caaatataag ggatgcgagt ccggaactga cataggtttc
     1921 accataaatc attaatgaga aaggaatgga aaaataaaaa atacaaatta caagctggaa
     1981 taagcgttgt ccgggtggaa
//
LOCUS       DAPGWD010000302         1897 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPGWD010000302 DAPGWD010000000
VERSION     DAPGWD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.305"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..134)
                     /locus_tag="RHX88_004472"
     CDS             complement(<1..134)
                     /locus_tag="RHX88_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2233062.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     "
     gene            201..728
                     /locus_tag="RHX88_004473"
                     /pseudo
     CDS             201..728
                     /locus_tag="RHX88_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            725..1721
                     /locus_tag="RHX88_004474"
                     /pseudo
     CDS             725..1721
                     /locus_tag="RHX88_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          459 a          375 c          460 g          603 t
ORIGIN      
        1 tgtgattcat acaggctgag ttcacgggct gcggcagtaa caccgatgcg ttcagcaagc
       61 ttcagggctt cactgcgaaa ttcaggcgaa tgctgtttac ggggtttttt actggttgat
      121 actgtttttg tcatgtgagt cacctctgac tgagagttta ctcacttagc cgcgtgtcca
      181 ctattgctgg gtaagatcag gcggttaaag gcatcaaccg cgaacaactg cagacgatga
      241 ttacggaatc aggcattgcg ctggatatta gctgtgataa cacgccgcgc cagcaggtta
      301 ttgggggaac gcaggcctcg ttgaatgaat ttgccacgct gctgatggcc gctggatacg
      361 aaccggttaa actcggtgtg agcggtgcct ggcacacgcg tttgatggaa gacggcgttc
      421 aggcaatgcg cgattatctt gccggactgg atatcgcttc gccagagcat caggttctga
      481 tgaacgtaac agcaaaaagc gaggtcgctc cgtcaattat caaagaaaat ctctcattac
      541 acttaaccca tacggtgaaa tggactgaat ctctcgatac atttctgaat atgccaaccc
      601 cggtagcttt ccttgaaata agtaataagc cctatttggg gaatatgtta aatgattttg
      661 ctggcgtgga tcagcaacga gttatgcatt gtcgtaaagc atttagtgat gcaaaggtat
      721 tcaaatgatt cctgattatt taacttttat tcgctttcag gataaacgaa atctgatata
      781 catttatgct attggactta ttctgatagg cttttattgg aaaaatgcag gatttacttt
      841 tccatcagag gatattggtg tcgttagtgg gattctggcc ctggtgctgt ataattttat
      901 ttttgatctc aaggcgtact gggcttataa atgcgtcacg aagaatatcg atttttcgtg
      961 gtttaagaaa aagcagaacc acaaaataga attatttctt acacaacctc tggtggcagg
     1021 atttctgtcg ttaatcatgt tgagtgcaat gagttggggg ctataccagc ttctaccctc
     1081 gttatatgcg ctgttcctga tttcgttact tgggccgttg gtcatctttc tgctgtttcg
     1141 gatgatccgc accagttatg tcaagcaggt cgctatttca gtagcgaaaa aagtaaaata
     1201 taaaagtctg actcgctatg tgctgctttc ggtgtgcatc tcaacggttg ttaacctgct
     1261 tactatcagc ccgttacgta acagtgattc ttttgtgaca gaggggcagt ggttaacgtt
     1321 taaatcgata attgcattgc tcattctttg tggcgtagtg ttggcgatta atctgttttt
     1381 tctgcgcttc tccacgtgac aaacagcggt acgcttttct gggcaggctt tttttgcagg
     1441 aaatcgatct gtttttctcc agtgaaaatg cgttgtcgac cttttttgcc aagccgcttt
     1501 ggcttcggtt attcatattg ctggttattg aaatgatgtg gattacgctg gtgtcggtat
     1561 tggcaacgct tgtagaatgg cggatttggt ttgaagccta ttttttactc tgctatgtac
     1621 cgtgcttaat ttactatttt ttccattgtc gatgcctctg gcataacgat tttatgatgg
     1681 catgtgacat gtatttccgt tgggggcatt ttaataagtg aggaagttac aggaagtgac
     1741 cagataacgc atatatgttc tctactatct tgcgcatttt gattgttgac tgagtaacca
     1801 gacagttgat gtgcacaatt tcccctcgcc ctaacagacg tgggcggggg cacccggtgt
     1861 gtgcaatata cgaaaaaaaa gcccgtactt tcgtacg
//
LOCUS       DAPGWD010000303         1882 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPGWD010000303 DAPGWD010000000
VERSION     DAPGWD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.306"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(68..1480)
                     /gene="pykF"
                     /locus_tag="RHX88_004475"
     CDS             complement(68..1480)
                     /gene="pykF"
                     /locus_tag="RHX88_004475"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDV2233063.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          477 a          441 c          460 g          504 t
ORIGIN      
        1 tatcgattaa ataaaaaaag cgcccatcag ggcgcttcga tatacaaatt aattcacaaa
       61 agcaatatta caggacgtga acagatgcgg tgttagtagt gccgctcggt accagtgcac
      121 cagaaaccat aactacaacg tcacctttgt gtgccagacc gctctgcaga gccagttctt
      181 tacccagacg gtagaaatca tcagtagaag tgatctcttt aaccagctgc ggcacaacgc
      241 ctttgctcag taccaactga tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg
      301 catccgggaa gtatttacgt actgcgcgag cagatttacc gccctgagta gcaaccacga
      361 tcagcggagc atccagtttt tcagcagttt caacggcacc acggcatacc gcttcggtaa
      421 tgcgcagttt acggttgtca ttgttgaact cgagacggct gttcatcacg cggtcggtac
      481 gttcgcaaat ggtcgccatg atagaaaccg cttccagcgg gtatttacct tttgcggatt
      541 caccagacag catcactgca tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt
      601 ctgcgcgagt cgggcgtggg tttttgatca tggaatccag catctgggtc gcagtgataa
      661 cgactttacg tgcacggata catttttcga tcatcatctt ctgggcgaag ataacttctt
      721 ctaccgggat ttctacaccc aggtcgccac gcgcaaccat gatgccgtca gaggcttcga
      781 ggatttcgtc gaagttgttg aggccttcct ggttttcgat tttggagatg atgtggatgt
      841 tttcgccgcc gtgcgctttc aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa
      901 taaaggaagc agcaacaaag tctacgcctt gttcgcaacc aaagatcagg tcctgtttgt
      961 ctttttcagc cagtgctggc agagcaatgg aaacgccagg caggttcaca cctttgtttt
     1021 cgcccaggtc accgttgttc agcactttac agataacttt gttaccttca atggcggtaa
     1081 cttccatacc gatcagacca tcgtcaacca gtacggtgtt gccaacagac aggtcagtag
     1141 tgaaaccttc atacgttacc gcaaccattt cgctgttgcc gataacagat ttatcagtgg
     1201 tgaaagtaaa ggtctgacca gctttcagag aaacgtcgtt accgccttcc agtttcatgg
     1261 tgcggatttc cggacctttg gtatcaagca ggatagcggc ggttttacca gttttgctca
     1321 tcacgttgcg cagattctga atgcgctgac cgtgttctgc atagtcacca tgagagaagt
     1381 tcagacgcat aacgttcatg ccagcgtcca gcattttagc taacatctct tcagattcgg
     1441 ttttcggtcc gatggtgcaa acaattttgg tctttttcat gacagtctta gtctttaagt
     1501 tgagaaggat gggagaaact tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta
     1561 cgaaagtggt ggtgcctcgc tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg
     1621 tgtgcttgtg tctcagccca acgtgagtgc ctgtttttga gtcgttgagt tctacagtcc
     1681 aaagtgctga aaccattcaa gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa
     1741 aggaaaacaa aaacagcgtt tcaaaaaata tacaacttgt tagacaataa attgttatca
     1801 aagatttaac gttccaggga aaaggttacg caaaaacttc actgtttgcg caacgtctgg
     1861 tgccgaagtt gtcagtgtga gg
//
LOCUS       DAPGWD010000304         1869 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPGWD010000304 DAPGWD010000000
VERSION     DAPGWD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.307"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            110..364
                     /gene="bssS"
                     /locus_tag="RHX88_004476"
     CDS             110..364
                     /gene="bssS"
                     /locus_tag="RHX88_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDV2233064.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            479..1597
                     /gene="solA"
                     /locus_tag="RHX88_004477"
     CDS             479..1597
                     /gene="solA"
                     /locus_tag="RHX88_004477"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDV2233065.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1645..1758
                     /locus_tag="RHX88_004478"
     CDS             1645..1758
                     /locus_tag="RHX88_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDV2233066.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          482 a          450 c          446 g          491 t
ORIGIN      
        1 ttaaaaattc ggtgaatacc cttacttatt ggtatactga agttgcttca gataaaaacg
       61 cattgaacct cgaataacgt tgtctagtaa cacgaattag ggggccatga tggaaaaaaa
      121 taatgaagtc attcagactc atccgctcgt agggtgggac atcagcaccg ttgatagcta
      181 tgatgcgctg atgttgcgtt tgcactacca gaccccaaat aagtccgagc aggaagggac
      241 tgaagttggt cagacgctct ggttaaccac tgatgttgcc agacaattta tttcgatatt
      301 agaagcagga atcgccaaaa ttgaatccgg tgatttcccg gtaaacgagt atcggcgtca
      361 ttaacatgac atgaccatcc acccaaaagg cacctcaccg gtgcctttac catttctggg
      421 ttgtatatct gcattcagtt aattactctg cttaaaccat gagagcagag agaaaaaaat
      481 gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc gctgccgcag ggtattatgc
      541 aacccgcgcc ggtttaaacg tgctaatgac cgacgcccat atgccaccgc atcaacacgg
      601 cagccaccac ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt
      661 cccgttggtc ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca acgaagatga
      721 tcccattttt gtacgctctg gtgtcattaa cctcggcccg gctgactccg catttctcgc
      781 caacgtcgcc cacagcgccg aacagtggca actcaacgtt gaaaagctcg atgcgcaagg
      841 gattatggcc cgctggccag aaatacgcgt cccggacaac tacatcggct tatttgagac
      901 tgattccggt tttttgcgca gcgaactggc gattaaaacc tggatccaac tggcgaagga
      961 agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca attcgtcatg acgatgatgg
     1021 cgtaactatt gaaaccgttg acggtgagta tcaggcgaaa aaagcgattg tctgcgcggg
     1081 aacatgggta aaagacctgc tcccggagct gcctgtccag cccgtacgta aagtatttgc
     1141 ctggtatcag gccgatggcc gctatagcgt gaagaataaa ttcccggcgt ttaccggtga
     1201 actgcccaat ggcgatcaat attatggttt tccggcagaa aacgacgcgt tgaagattgg
     1261 caaacataac ggaggccagg ttatccattc agcggatgaa cgtgttccgt ttgcggaagt
     1321 ggtcagcgat ggttcggaag ccttcccgtt cttgcgcaat gtattgccgg gtatcggttg
     1381 ctgcctgtac ggcgctgcct gcacctatga taattcgcct gacgaagatt ttattatcga
     1441 taccctaccc ggccacgata atacactgct cattaccggc ctgagtgggc acggttttaa
     1501 atttgcgtca gttttagggg aaatagctgc cgattttgcg caagacaaaa aaagcgattt
     1561 tgatttgacg ccattcaggc tttcccgctt ccaataatca tcattacggc ctccagaatg
     1621 aggccgtttt tacaggagta tttaatgcgt cggctgttgc actatctcat caataatatt
     1681 cgcgagcatc tgatgcttta tctttttctc tggggattgt tggcaattat ggatctgatc
     1741 tatgtgtttt atttttagtt ttccgcactg acataaactg acaaatattt aatctccctt
     1801 tcatttggcg tgaaaaattg aatgagatta ttcctgcttc tacagcactc accagacatc
     1861 tctgatgct
//
LOCUS       DAPGWD010000305         1858 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPGWD010000305 DAPGWD010000000
VERSION     DAPGWD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.308"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1847)
                     /gene="traD"
                     /locus_tag="RHX88_004479"
     CDS             complement(<1..1847)
                     /gene="traD"
                     /locus_tag="RHX88_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDV2233067.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAASLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDV"
BASE COUNT          458 a          513 c          452 g          435 t
ORIGIN      
        1 acgtcttctc cggaaacaac ctccgggaca tccggttcga agaggctggc catctgacga
       61 ccttctgctt cccttgcggc aagtacggca ctcagacggt tctccatttc cgggttgatg
      121 tcacgcggaa taaactccgg agcgaccttc ggtcgtgtct gatatttcag agagagtttt
      181 actgccggat acggtccggg cagggtgaca taacaggtca gatccggcag agactgaatg
      241 tcggaataac tgaccagcgt ctggcgctcc atatccttac cggtcgatac cccgtcacgt
      301 accggatcag caccgtagga atactgctcg ctggctttca ggtgctcttt ctcaccaatt
      361 tcaccggcag cgaactctgc aatcttatgg ctgggagaac ggaaaaaggc acgggtgttc
      421 atgacgtcaa acagcgaggc agccgctttc tcaccgtaga tatcttccag ctgggcatag
      481 gactggatac caaacacata acagccaccg aacttacggg cttccggcag gatctccacc
      541 aggtccggca gtttgtgtaa cgtgggtaac tcatcacaga aaaaccacac acgacggtta
      601 cggttttctc ccattgccag cagaccacga atggcaatgg acagccacat ggagatcacc
      661 ggcttcaggg aggcatgggt gtcggcattc gacgaaataa acagccagcc gtttttctga
      721 tcttcccgga caccacgcat ccagtcacgg atggtaaaag actcaccgtt atgctcaatc
      781 ccctgcaggt aacggatggc tttcacgtag ttggtcagca cagcacggat ggaaatcgcc
      841 gttttctcaa ttttctcttc caccaggttg gccgccggtg aattacgcag gaaggtacgc
      901 agtttttcga ttttgatgga aagcagtgtg tccaccagtt tgctgtagct gcggttgggg
      961 tcattacgca tcaggtacgc cgcttccgca aaaatggtac gtcctgaccc ctgccagaac
     1021 gggtcctctt ttgtgcccat cgggatcagc gtgttcgcgg tattatcaaa atccggctgt
     1081 gtcaggcact ccttccacaa atcccaggcg gcacagcggg catccagcgg attcaggatt
     1141 ttatcgatgg agggatcata gtaactttta acaaattccc ctgaacggtc ataaatcacc
     1201 accatatctc cacgctgacg ggcgtagttg gccagacgac ggataacttc cgacttaccg
     1261 gccccgaccg tgccgtgcag acagaagttc tggatttcgg aatcccggat aatcggcagg
     1321 tcgccaatcc gtatatcgga gtccttgccg tcttttttca gcatccgggc aacgtctttc
     1381 ggattgtctg tcagctgacg accacctgtg acttcatttt cgctctgttg tttaccctga
     1441 cgccccagaa tccaggagac aacaaagaag gtaatcaggc aaataaccag tgccacaact
     1501 gtggccagaa cgaatgcgga ccatagctgc tctccgcacc agatcatata tttatcatgc
     1561 agtacctgag cagcattcat ccggaaggtt ttgccgtaat actggatctc atataccggc
     1621 tgggacttga ttaaatcccg catgccttcc agcgtggtac accaccagta aatacagccg
     1681 ttcacaaacg tctgccagct tatttttacc cataaaacca gaccaacgag tatccagaaa
     1741 aaaataaaca ggcagtaaag catgatattg gcgatctggc tgaacatgcg gatacgcatg
     1801 gacgcaatct gaccgccctg ggtcatatcc tttgcgttaa aactcatgat gatattcc
//
LOCUS       DAPGWD010000306         1853 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPGWD010000306 DAPGWD010000000
VERSION     DAPGWD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.309"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            162..665
                     /locus_tag="RHX88_004480"
     CDS             162..665
                     /locus_tag="RHX88_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDV2233068.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(744..995)
                     /locus_tag="RHX88_004481"
     CDS             complement(744..995)
                     /locus_tag="RHX88_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDV2233069.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1110..1196)
                     /gene="azuC"
                     /locus_tag="RHX88_004482"
     CDS             complement(1110..1196)
                     /gene="azuC"
                     /locus_tag="RHX88_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDV2233070.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1459..1782
                     /locus_tag="RHX88_004483"
     CDS             1459..1782
                     /locus_tag="RHX88_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDV2233071.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          478 a          437 c          410 g          528 t
ORIGIN      
        1 ttcttatacc cgttcagacg ttattcttat ttcagatcat cgtcagaatt gactccacga
       61 tcacatttcg gaccggcaga aaggaattat tctgcaaaca gtaattatgg tgttttgatt
      121 tatcttgcac ctctccactt ctggatataa ggatattagg tatggcaacc gctggaatgc
      181 ttctcaaact caactctcaa atgaaccgcg agttttacgc atccaatctc taccttcacc
      241 tgagtaactg gtgttctgaa cagagtctga acggcaccgc cactttcctt cgcgcccagg
      301 cacagagtaa tgtgacccaa atgatgcgca tgtttaactt tatgaagagt gtcggcgcta
      361 cccccatcgt taaagccatt gatgttcccg gtgaaaaact gaactctctg gaagaactgt
      421 tccaaaaaac gatggaagaa tacgagcaac gttctagtac gttggcacag ttagccgatg
      481 aagcgaaaga actgaatgat gattcaaccg tcaatttcct gcgcgatctg gaaaaagaac
      541 agcagcatga tggtctgttg ctgcaaacca ttcttgatga agtgcgcagt gcgaaacttg
      601 cgggtatgtg ccctgtgcag accgaccaac atgttctgaa tgtcgtgtca caccagctgc
      661 attgatcatc atcggcgcta atgcattgcg ccgatgaagg ttttgagaaa ccgctgcctc
      721 atctgtttga agcagcggtt tttttaatgg gattcaccct gtggggtaaa cttgagttca
      781 ataagcgcga tggctttttg gattgcccgc atggtgaccg ggtctgcggc ggcgggatgg
      841 ttagtaaagt cgatattctt cagctgactg gacatttttt cacgaacttc aacgggcgcg
      901 attacatcga gaacatccag aatttgtttg ataaccaact ggcaagcaac cacatcagaa
      961 accagttcct gatcggcatt cagcggctgg gacatcgtaa actcctgata gcattttgaa
     1021 agccgttata gtagcggctt cacatcttca gcgatagtca catccaccgt catcaggaca
     1081 caaaaaaacc tgccggagca ggttttttat tatcggaaca tattgcctgg cggtacgtct
     1141 ttgaacgtct tgcaatagtt attgaacata cttttcagga ttttgcgcag tttcatcgcg
     1201 gcactccgac catttgttat acaggtgtta ttgtctttgc gcttataata tgacaaccat
     1261 cacaaaaatc aatctttatg tgatacaaat cacataaata ctcctttaat gttataaaaa
     1321 tgataatcaa aaaacagccc ccctatttct gacacctaca gatggcaaga aatagcgcct
     1381 gccaggcgtc ttttccggcc attgtcgcag cgctgtaacg cgtaaaatag tgctttctct
     1441 tactcttctg gctggaccat gagacttctg attctgactc tttcattaat aacgctggcg
     1501 gggtgtacgg ttactcgcca ggcccatgtg agcgaagttg acgcggcaac aggtattgta
     1561 cggttggttt atgaccaggc ttttttgcag catgcccata ccgatcgtta tgtcagtcgc
     1621 gggatcgccg atcgcgcatg ccagcaggaa ggctataccc acgcggtccc ctttggtcag
     1681 ccagtaggca actgtagcct ttttgcgggt tccttatgtt tgaatacgga attcactttg
     1741 tcatatcagt gccatcattc cgccttccct gtctttctct aaatatttta atcagcgagg
     1801 ggatcttcgc tgattaaaga aatagcggta atgcgtttta ttcccattcg tat
//
LOCUS       DAPGWD010000307         1804 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPGWD010000307 DAPGWD010000000
VERSION     DAPGWD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.310"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..695)
                     /gene="emrY"
                     /locus_tag="RHX88_004484"
     CDS             complement(<1..695)
                     /gene="emrY"
                     /locus_tag="RHX88_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDV2233072.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLD"
     gene            complement(695..>1804)
                     /gene="emrK"
                     /locus_tag="RHX88_004485"
     CDS             complement(695..>1804)
                     /gene="emrK"
                     /locus_tag="RHX88_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDV2233073.1"
                     /translation="LLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVS
                     GSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSA
                     EVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANK
                     ALIMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSL
                     MAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFS
                     LLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELA
                     STVTSMPAYTSKALVIDTSPIEKEIGNIISHNGQL"
BASE COUNT          510 a          411 c          359 g          524 t
ORIGIN      
        1 caatccagat cgcgcccttt atcaagcata atttgcaagc caccaacacc gagcactaac
       61 agggtcagtc ctggtagatt catttttacc ggtgaagttt cagtttctct tcctttaagt
      121 aaggttaagc ataatgtcag gacgataatc cccataggga cattgattaa aaatatccaa
      181 ccccagctaa agttatcgca aatataaccg cccaatatcg gcccacatat cggagcgata
      241 atcacggtca ttgaccataa tgccagagca aatgttcttt tttctggtgg ataattcctc
      301 aataataaac tctgtgacag tggaattaac ggccccgcca ttaatccctg aacgactcta
      361 aaaaatatca gcacatcaag attggtcgat aaactacaca ttaaggaaga cagcgaaaaa
      421 aaagtgactg aaagtaaaaa taaccttaat tcgcctattc tttgtgccag cctgccggta
      481 acagggatcg caatggcatt tgctacgcca aacgaggtga taacccaggt gccttcgtct
      541 gttgatgctc ccagaaagcc agatattgtc ggtattgcga cgttagaaat agtggaatcc
      601 aacatttgca taaatgtcgc taatgacaat gcaatagtga cgcaccataa cgtcccaccg
      661 gttaatggtg ccggagttga tttagtgatt gccattaaag ttgtccatta tgcgaaataa
      721 tgttgccaat ttctttttct atcggactgg tatcgataac taaagcctta ctggtataag
      781 ccggcatgga ggtcacggtt gaagccagct caggcatctc ggcaatgtct tcgttcttcg
      841 tatcaatagt tgctgtcatc gataaaccaa tacgcaaggg atgttccatg agttcttttg
      901 gatcaagaga aacttcaacc ggtacacgct gaacgatttt gatccagttc cctgtcgcat
      961 tttgtgcagg taataaggag aacgcattgc cggttcccat attgatccct gtcacccgac
     1021 catgaaacac aacattttca ccataaagat cgctgataat attgaccgat tgaccaatcc
     1081 gtacatccgt aagttgtgtt tctttaaagt tggcattaac ccacatttga cgtgccggta
     1141 cgacagccat taacgattgt ccggggctca ctgtttcgcc gacctgaaca cttctctggg
     1201 caatatagcc ggtaaccgga ctcttaatat ccgtacgttt aagcgccaac caggcttctt
     1261 tagttgcatc cgccgcttca acgacttgtg gctgacggtt taatggtgtg ttcattatta
     1321 aagctttatt cgctttataa gcctggatag cggcattcaa tgccgctttg ctacttatta
     1381 acgtatcttt ggtatgctcc agcgtttctt ttgaaataac cccctgcttc gctaacggca
     1441 ctcgacggtt ataatcttct aaagattgtt gatactgaat acgtgctgaa gcgacttcgg
     1501 cactgtattg tttatcctgt aagtatagtt tattcgtttg ccgaacaata tttgccagat
     1561 tatttttagc tttattgagt gcgatagtgg catcagtttt atccagtgaa actaaaatgt
     1621 caccttgtcg aacgtagttc gtatctttat gattaacgac agtgacacta cctgagactt
     1681 gtgcagaaat tggatctgca ttccccgtga cataggcgtc atctgtacta atcatgtctt
     1741 ctaattccat tgaccaatag gcataggcac ctgaaaacgc aataaataaa actaccgcca
     1801 ataa
//
LOCUS       DAPGWD010000308         1786 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPGWD010000308 DAPGWD010000000
VERSION     DAPGWD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.311"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            85..1728
                     /gene="yojI"
                     /locus_tag="RHX88_004486"
     CDS             85..1728
                     /gene="yojI"
                     /locus_tag="RHX88_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDV2233074.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLIQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT          391 a          432 c          506 g          457 t
ORIGIN      
        1 aaagaataaa aataagaatt attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg
       61 taattcattg tccgggtttt ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc
      121 tggccattta tcagtgtgat ggcgctaagc ctcgccagtg cggcattagg cattggctta
      181 attgctttta tcaatcagcg ccttatcgaa acggcggata ccagtctgct ggtgttgccg
      241 gagtttctgg gattattgct gctgttgatg gcagtcactc tcggatcgca actggcgctc
      301 accactttgg ggcatcactt cgtttaccga ctgcgtagtg aatttatcaa gcggattctg
      361 gatacccacg tcgagcgcat tgaacaactc ggtagcgcct cgttgctggc gggattaacc
      421 agcgatgtgc gcaatatcac cattgctttt gtgcgtcttc cggaactggt gcaggggatc
      481 attctcacta tcggttccgc ggcgtatctg tggatgctgt cgggcaaaat gttgctggta
      541 acggctatct ggatggcgat caccatctgg ggtggttttg tactggtggc gcgggtgtac
      601 aaacatatgg cgaccctgcg tgaaaccgaa gacaagctgt acacggattt tcaaaccgta
      661 ctcgaagggc gcaaagagct gactctgaac cgggaacgcg ccgagtatgt gtttaacaac
      721 ctctacatcc ccgatgcgca agagtatcgc catcatatta ttcgcgcaga caccttccat
      781 cttagcgccg tgaactggtc aaacatcatg atgctgggcg ctatcggcct ggtgttctgg
      841 atggcgaaca gtctcggttg ggctgatacc aacgttgctg cgacctattc gttgacgctg
      901 ttattcctgc gtacgccgct gctttcggcg gttggcgcat tgccgacgct gctgacggcg
      961 caggtggcgt ttaacaagct gaacaaattc gcgctcgcgc ctttcaaagc tgagtttccg
     1021 cgcccgcagg cttttcccaa ctggcaaacg ctggagctgc gtaacgtgac gtttagctat
     1081 caggataacg cgttttccgt tgggccgatt aacctcacca ttaaacgtgg cgagctgctg
     1141 tttctgattg gtggcaacgg tagtggaaaa tcgacgctgg cgatgttgct gacgggcttg
     1201 taccagccac aaagcggcga aatcttgctg gatggcaaac ctgtcagtgg cgaacaaccg
     1261 gaagattatc gcaaactgtt ttcggcagtg tttaccgatg tctggctgtt tgatcaactg
     1321 ctggggccgg agggtaaacc cgctaacccg caactggttg agaagtggct ggcgcagctg
     1381 aaaatggctc ataagcttga gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa
     1441 gggcagaaaa aacgcgtggc gctgttgctg gcgctggcag aagaacgcga tattatcctg
     1501 ctggatgaat gggcggcgga tcaggatcca cacttccgtc gtgagtttta tcaggtgttg
     1561 ctgccgctga tacaggagat gggtaaaact attttcgcta tcagtcatga tgatcattac
     1621 tttatccacg ccgaccgcct gctggaaatg cgcaatgggc aacttagcga gctgacgggc
     1681 gaagagcgcg atgccgcttc gcgtgatgcc gttgcccgga cggcataata ttacgacgct
     1741 gacctggcgg actgctgccg tcaggtcaat atgctaaaaa tccctt
//
LOCUS       DAPGWD010000309         1776 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPGWD010000309 DAPGWD010000000
VERSION     DAPGWD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.312"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1320
                     /locus_tag="RHX88_004487"
     CDS             <1..1320
                     /locus_tag="RHX88_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDV2233075.1"
                     /translation="SDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQV
                     AYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLSA
                     NIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNK
                     EEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNY
                     GIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINI
                     TNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESE
                     TELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGV
                     VGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1333..1776
                     /locus_tag="RHX88_004488"
                     /pseudo
     CDS             1333..1776
                     /locus_tag="RHX88_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          564 a          350 c          342 g          520 t
ORIGIN      
        1 tccgataact ataatgctat aactctggga aatggttgga atacaccgct gggtgccatc
       61 tctttttacg ctgcccgatc gagtagtaag ttaaataatg ataccagaca cgaaggtacg
      121 agctatcagg tcgcctataa taaatatcta gtacaaacag ccactcgatt tagcgtggcc
      181 gcctggcgtt atgcttcgca ggattacagg acattcagcg accatcttta cgaaaatgat
      241 aaacactatc atcagagtga ttatgatgat ttttatgata ttggcagaaa aaatagcctt
      301 tctgctaata ttatgcaacc tttatccaat aatctgggaa atgtatcatt aagtgcgctt
      361 tggcggaatt actgggggcg aagtggaaat gctaaagatt accaattcag ttactccaat
      421 aactggcaac acattagtta tactttctct gccagccaat cttatgatga aaataataaa
      481 gaagaggagc gttttaatct gtttatctcc attcctttct actgggggga tgatattgcc
      541 aaaacacgtc accaaattaa cttatcgaat tcgacctcat tttccaaaga tggctattcc
      601 tccaacaata ctggaattac tggcatagcc ggtgaacatg atcagttaaa ttatggtata
      661 tatgttaatc agcaacaaca aaataatgat acctcgcttg gtacgaattt aagctggaga
      721 actcccatcg ccataataga tggcagctat agtcattcta aaaacgcctg gcaaagtggt
      781 ggaagtatta gtagtggatt agttgtctgg cccggtggta ttaatatcac taaccagtta
      841 tccgatacat ttgcaattct ggatgcgcct gggttagaag gtgcacatat taatggacaa
      901 aaatacaatc gaacaaacag caaaggccag gttgtttacg acctgataat acctcatcgt
      961 gaaaaccatc tggtacttga tatagcaaac agtgaaagtg aaacagaatt gcagggcaat
     1021 cgtcaaatta ttgcacctta ccgtggagca gtttcttatg tgcagtttac aactgaccaa
     1081 cgtaagccct ggtatataca ggcactgcgt cccgatggtt cgccattaac ctttggctat
     1141 gacgtactgg atctccagga aaataatatt ggagtcgttg gccagggtag tcgccttttt
     1201 attcgcgtag atgaaattcc cactggaata aaagttgctc tcaatgatga acagaactta
     1261 ttctgtacta ttacttttca acacgttatc gatgaaaaca aaacatatat atgccagtaa
     1321 aaggagttta ctatgaagtg gcttttatta atcaccttga gtctatattc ttttattgtg
     1381 caaagtgctc cctgtgcatt gaccaatgtc ggtgaacaac gtggaacata tatactcaag
     1441 ccactgagta tgaaaggtaa tctaaccgcc gaaaaattga aaacaccgga aatatcattg
     1501 agtgtacaga cttgcaagag acaaccaacg ccattaaagt tcatttttta acacagaacg
     1561 ctttaaggtt taaagtcgga agtcgtcatt atattattaa tttccccttt gaatctgacg
     1621 gtaaaaaaat agagcttcca gggaagtcat cttataattt agaagaaatg ctatctgcga
     1681 taaatttcac cattccatat tctatcgcat ctgtatcaga tagttctaac accgcagaca
     1741 tttttcctgg aactcctttt cctttgttgt tcgaaa
//
LOCUS       DAPGWD010000310         1766 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPGWD010000310 DAPGWD010000000
VERSION     DAPGWD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.313"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            70..1101
                     /locus_tag="RHX88_004489"
     CDS             70..1101
                     /locus_tag="RHX88_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDV2233076.1"
                     /translation="MFKNKVLLITGGTGSFGNAVLQRFLDSDIGEIRIFSRDEKKQDD
                     MRKKYASDKLKFYIGDVRDYSSVLTATRGVDFIYHAAALKQVPSCEFYPMEAVKTNVI
                     GTDNVLEAAIANKVSRIVCLSTDKAVYPINAMGTSKAMMEKVIVAKSRNLPKDITICA
                     TRYGNVMASRGSVIPLFINQILEGRPITITDPCMTRFMMTLDDAVDLVLHAFEHGTNG
                     DIFVQKAPAATIDTLTKSLLKLTKQHSHPINIIGTRHGEKLFEVLCSREEMLVAEDQG
                     NYYRIPSDKRDLNYEKFFDKGTKEIQFVEDYNSHNTRRLDVDEMVALLRKLDYINKIE
                     AGEKADPDA"
     gene            1094..>1766
                     /locus_tag="RHX88_004490"
     CDS             1094..>1766
                     /locus_tag="RHX88_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2233077.1"
                     /translation="MRKKILIVGANGMLGSSLLRYFSSIGDYEVLGTTRSMVVAKQLE
                     QKHNVKIIDNVDVIDFKRLETVVVEHKPNIVFNCVGIIKQLDAAKNNILSIEINSLLP
                     HKLAQLCSAHSAKLIHFSTDCIFKGTKGNYVEDDESDAIDLYGKSKFLGEVEYNGHLT
                     LRTSIIGHELGSNHSLVDWFLSQKKSVKGFTNAIFSGLPTCYMAEVIHKYVLPNNLAG
                     LFHLSV"
BASE COUNT          571 a          271 c          351 g          573 t
ORIGIN      
        1 aatagctata tgtattgtcc gaaaacaagc tgggcagatt tcttcgagtt aatctaattg
       61 aggtatacga tgtttaaaaa taaggtactt ctcattactg gtggcacagg ttcctttggg
      121 aatgccgttt tacaacgctt tttagattca gatattggcg aaattcgaat ctttagtcgt
      181 gatgagaaaa aacaagatga tatgcgtaaa aaatacgcaa gtgataagtt aaaattttat
      241 ataggggatg tgagggacta tagcagtgtt ttaacagcga ctcgtggggt tgattttatt
      301 tatcatgcag cagcattaaa gcaagtacct tcatgtgagt tttatccaat ggaagctgtc
      361 aagactaacg ttattggaac tgataacgta cttgaagctg caattgcaaa caaagtttca
      421 cgtattgttt gtttgagtac cgataaagct gtttatccaa ttaatgctat gggtacatct
      481 aaggctatga tggaaaaagt tatcgtagct aagtcaagaa acttacctaa agatattact
      541 atttgtgcga cgcgatatgg aaatgtaatg gcttcccgtg gttcggtcat ccctctgttt
      601 attaatcaaa tacttgaagg acgcccaatc actatcaccg atccgtgtat gactcgattc
      661 atgatgactt tggatgatgc tgtagatctt gtattacatg cttttgaaca tggtactaat
      721 ggtgatattt ttgttcaaaa ggcaccagct gcaacaatag atacacttac caagtcatta
      781 cttaagttaa ctaaacaaca ttctcaccca attaatatca ttggcactcg ccatggtgaa
      841 aaactatttg aggtactctg cagtcgggaa gaaatgttgg ttgcagaaga tcaaggtaat
      901 tactatcgta tacctagtga taaacgtgat ctaaattatg aaaaattctt cgacaaaggg
      961 actaaagaaa ttcagtttgt tgaagactat aattctcata atacccgtcg tttagatgtt
     1021 gatgaaatgg ttgctcttct tagaaaatta gattacatca ataaaattga ggctggtgaa
     1081 aaggcagatc ccgatgcgta aaaaaattct aattgttggc gcaaatggca tgttaggtag
     1141 tagtttacta cgctactttt catcaattgg agattatgaa gttcttggta cgacaagaag
     1201 tatggtcgtt gcaaaacaac ttgagcaaaa gcacaatgtg aaaattattg acaacgttga
     1261 tgttattgat tttaaacgat tagagactgt agtagtagag cataagccaa atattgtttt
     1321 caactgcgtt gggataatta aacaacttga tgcagcaaaa aacaatatat tatctattga
     1381 aattaactca ttacttccac ataaattagc tcaattatgt tcagctcata gtgctaaact
     1441 catacatttt tcaacagatt gcatttttaa aggtaccaaa ggtaattatg ttgaggatga
     1501 tgagtctgat gcaattgatt tatatggtaa atctaagttc ttgggtgaag ttgaatataa
     1561 tgggcattta actttgcgga cttctattat tggccatgag ttgggatcaa atcatagtct
     1621 tgttgactgg tttttatcac agaaaaaatc ggtgaaagga tttactaatg cgatattctc
     1681 aggcctccca acttgttata tggcagaggt tatccataaa tatgttcttc ccaacaatct
     1741 tgctggttta tttcatttaa gtgtag
//
LOCUS       DAPGWD010000311         1761 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPGWD010000311 DAPGWD010000000
VERSION     DAPGWD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.314"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..272)
                     /locus_tag="RHX88_004491"
     CDS             complement(<1..272)
                     /locus_tag="RHX88_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233078.1"
                     /translation="MIQYLVKNQVDRIQCNDTGKRIYETLAYLYKGKPTPLKYSDVLH
                     RAACSEDGLKFWLRQLSNFGVIEIKELSFSTFNLKRLDKEIDFIYST"
     gene            complement(284..937)
                     /locus_tag="RHX88_004492"
     CDS             complement(284..937)
                     /locus_tag="RHX88_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2233079.1"
                     /translation="MINEEQFVKVPMWILKISQVNGHKFTDSAKNLYCYLKGFDRAFP
                     SYAKIGDVFGITPRAAEERVKKLLEMGLIIKDARPGSSNVYQVVDIPQEEARQEAQTQ
                     QEATVKAVPDPLPPTEDNKQPSFDVLDDWDAPLPWETEETPVSSSEKVANDNEEDVLE
                     NFASLICQQKSTRTGGASFIEFATSLACKHRIKLPNGIEAYFAKKHPKVYEDFDIPF"
     gene            complement(989..1156)
                     /locus_tag="RHX88_004493"
     CDS             complement(989..1156)
                     /locus_tag="RHX88_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233080.1"
                     /translation="MSETKKPIPRTYLHVDPEIFKVLFAEAKKRQIMVSDLMLEIITE
                     AAENIKQKKGK"
     gene            complement(1179..1367)
                     /locus_tag="RHX88_004494"
     CDS             complement(1179..1367)
                     /locus_tag="RHX88_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233081.1"
                     /translation="MKYFTSQDVVEAWKRGEINRFKVRMYRNTARRCGYPEREKCFDD
                     ALKIIDELRKAEKEAEKE"
BASE COUNT          483 a          361 c          300 g          617 t
ORIGIN      
        1 gtggaataga tgaagtctat ttctttatcc agtcttttga gattgaatgt agagaaagat
       61 aattctttaa tctctataac accgaaattt gatagctgtc taagccagaa ttttaaacca
      121 tcttcagagc aagctgctcg atgtaacaca tcgctatatt ttagcggtgt tggctttcct
      181 ttatagaggt aagcaagtgt ttcgtagatg cgttttcctg tgtcattaca ctgaattctg
      241 tctacttgat tttttactaa atattgaatc ataaccctcc ttattaaaat ggaatatcaa
      301 agtcttcata tactttggga tgtttcttag caaaatatgc ctcaatacca tttggtagct
      361 ttatcctgtg cttacaggct aacgatgttg caaattctat aaatgatgcc cctcctgttc
      421 tagtactctt ctgctggcag ataagagatg caaaattctc taacacatcc tcctcattat
      481 cattagctac cttttcactt gatgaaacag gtgtttcttc cgtctcccac ggcaaaggcg
      541 catcccaatc atcaagaaca tcaaaagaag gctgtttatt atcttccgta gggggaagcg
      601 gatcaggaac agctttaaca gtagcttctt gttgtgtctg ggcttcttgt ctagcttctt
      661 cttgtggaat atctaccact tgatagacat tagaacttcc tggtcgtgca tcttttataa
      721 ttaaccccat ttcgagcaat ttcttaaccc tttcctctgc tgctcttggt gttatgccaa
      781 aaacatcgcc tatcttagcg taagagggaa atgcacgatc aaaccctttc agatagcagt
      841 aaaggttttt agcactatct gtaaatttat gcccgtttac ttggcttatt tttagaatcc
      901 acataggaac ttttacaaat tgttcttcat taatcattaa aaatctcctt ataaatcacg
      961 cttaatgcgc tactaaagtg aatgaggatc acttaccctt tttttgtttg atgttctctg
     1021 ctgcttcagt aatgatttct aacatcaaat cactgaccat aatttgcctt ttcttggctt
     1081 cagcaaataa aaccttgaag atttcagggt caacgtgtag gtaagtgcgc ggaattggtt
     1141 tcttagtttc gctcatagtt ttctccttct cttgtctatt attctttctc agcttctttc
     1201 tcagctttac gcagttcatc aataattttc agtgcatcgt caaaacattt ttcacgctct
     1261 ggataaccac aacggcgagc ggtgtttcta tacatcctta ctttaaagcg gttgatctca
     1321 ccacgtttcc aggcttcaac tacatcttga gaagtgaaat acttcactaa ttaacctcct
     1381 attctatttg gttaaaagaa aaaacacctt gcaagccttg ggcttcaaag tgcggtgcat
     1441 tggtttaatt atttagagga taagaatcta ttcctgtccc ctctaattcc tattattgca
     1501 cagaaagaga cgtttgtcaa taccccttcc gaaaaaaaat cgatcttttt catgcactta
     1561 cgagcaactc gtgctcttct gtgtagtgcc attcttcatt attgtctaaa acgctctaaa
     1621 acgcgttata acgagatttt gataaaagca atacattccc ttatgtaatg gctgtttagc
     1681 tcgccagagg cttatttaag gggcttattt tgtatttgtt aggtatatga aaaggtaatg
     1741 gtagatataa gatagagagt c
//
LOCUS       DAPGWD010000312         1760 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPGWD010000312 DAPGWD010000000
VERSION     DAPGWD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.315"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            93..308
                     /gene="essD"
                     /locus_tag="RHX88_004495"
     CDS             93..308
                     /gene="essD"
                     /locus_tag="RHX88_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV2233082.1"
                     /translation="MKSMDKLTTGIAYSTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGMLTYLTNLYFKIKEDRRKAARGE"
     gene            313..624
                     /locus_tag="RHX88_004496"
     CDS             313..624
                     /locus_tag="RHX88_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HDV2233083.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFGLDVAMDR
                     VEGAALEFYEAAARMSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            621..1154
                     /locus_tag="RHX88_004497"
     CDS             621..1154
                     /locus_tag="RHX88_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV2233084.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKNVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1151..1648
                     /locus_tag="RHX88_004498"
     CDS             1151..1648
                     /locus_tag="RHX88_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HDV2233085.1"
                     /translation="MNQIFTVILLVLSGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRSISRDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
BASE COUNT          510 a          358 c          452 g          440 t
ORIGIN      
        1 ttgaatagat gttcgggcct tacgctaatg taacttcttt ccttcttcga accaaaccgt
       61 gtacaccatc cgttatctgc ggaggtgagg ctatgaaatc catggataag ttaacaacgg
      121 gcatcgccta cagcacctct gcaggcagtg ctggctactg gtttttacag ttgctcgata
      181 aagtcacgcc ctcacagtgg gcggcaatag gtgtattggg tagtctggta tttggcatgc
      241 tgacgtatct gacaaatctt tatttcaaga ttaaagaaga caggcgtaag gctgcgagag
      301 gagagtaatc caatgactca agactatgaa ctggttgtga aaggagtccg taattttgag
      361 aataaagtta cggtaactgt agccttacag gacaaagaac gctttgacgg tgaaattttt
      421 ggcctggatg tcgccatgga ccgtgttgaa ggagctgcgc tggagtttta tgaggcagca
      481 gccagaatga gcgtccggca agtcttcctg gaagtagcag aaaaattgtc agaaaaagtt
      541 gagtcttatc tgcagcatca gtactccttt aagattgaaa atcctgccaa taagcacgag
      601 cgtcctcatc ataaatatct atgaacacaa aaatcagata cggcctgtcg gctgccgttc
      661 tggcgctgat tggtgctggc gcatctgctc ctcagatact tgaccagttt ctggacgaaa
      721 aagaaggtaa ccacacaatg gcataccgcg atggttctgg catatggacc atctgtcggg
      781 gtgccacagt ggtggatgga aaaaacgttt ttcccaatat gaaactgtcg aaggaaaaat
      841 gcgaccaggt caacgccatt gagcgtgata aggcgctggc atgggtggag cgcaatataa
      901 aagttccact gaccgagcca cagaaagccg gtatcgcgtc attctgtccc tataacattg
      961 gccctggtaa gtgttttccg tcgacgtttt ataagcggct taatgccggt gatcgtaaag
     1021 gcgcatgcga ggcgattcgc tggtggataa aggacggtgg acgtgattgc cgcattcgtt
     1081 caaataattg ttacggtcag gttattcgtc gtgaccagga gagtgcatta acctgctggg
     1141 ggatagaaca gtgaatcaga tattcacggt gattttgctc gtgttgtcag gatttgtcgt
     1201 aggtaatgtc tggagcgaca gaggatggca aaaaaaatgg gcggagcgtg atgctgccga
     1261 attatctcaa gaggtaaatg tccaatttgc tgctcgaata attgaacagg ggcgaagtat
     1321 atcccgtgat gaggctgtta aagatgcaca acagaaatct gctgaaattt ctgccagggc
     1381 tgcttatctg tctgatagtg ttaaccagtt gcgtgccgaa gcaaaaaaat atgccatacg
     1441 ccttgacgca gcgaagcata ccgcagatct tgccgctgcc gtcagaggca aaacaaccaa
     1501 aaccgccgaa ggaatgctca ccaacatgct cggagatatt gcagcagaag ctcagcttta
     1561 tgctgaaatt gctgacgaac gctacatcgc aggagtgact tgtcaacaga tctatgaatc
     1621 tttaagagat aaaaagcatc aaatgtaggg taatattaaa tcggaacatt tacatcgcgg
     1681 aatgtaaaat ttaaataaaa aggactcttc catgagccaa aattcctgaa atcttaaggg
     1741 taagataaaa ggtcttaatc
//
LOCUS       DAPGWD010000313         1740 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPGWD010000313 DAPGWD010000000
VERSION     DAPGWD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.316"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(165..758)
                     /gene="tehB"
                     /locus_tag="RHX88_004499"
     CDS             complement(165..758)
                     /gene="tehB"
                     /locus_tag="RHX88_004499"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDV2233086.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(755..>1740)
                     /gene="tehA"
                     /locus_tag="RHX88_004500"
     CDS             complement(755..>1740)
                     /gene="tehA"
                     /locus_tag="RHX88_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDV2233087.1"
                     /translation="DKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGLVIL
                     AMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFRPLA
                     VCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALGYTD
                     AGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSVNGG
                     EGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGSGSE
                     NGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
BASE COUNT          493 a          468 c          384 g          395 t
ORIGIN      
        1 ctaagtcaat catcagtagt taaatttcag taagttaagc tgctaaaaaa cttcgactac
       61 tgtgattaat ttcacatccc ttcgataaaa aatatgctta ccgaaaagaa gaacagtaaa
      121 tcatctcagc gtggggaatt ttcacgcgcc ttattcttag cgggtcattt tttacgtgcc
      181 agcatcgtgg cgaaacgcag tttaatacga ttaccgttgg cgtcggtgcg gtgcagctcg
      241 ccgacgtctt cattgtattt caccctctcc cagccttcgt aatatcgacg taattctccc
      301 tctttgaagg caaacgggaa gccgacggta catggataat cagcggtatc catcgccgcc
      361 acaatcaggt tgtaaccacc aggtttagtg caacgttgca tattggcaat caacccgggg
      421 atggttttag cctcaaggaa catcagcacc acagtcgaaa gaataaaatc gtactgtcca
      481 tcaaatgtga ggttattcag atcaacgacc cgggtgtgta aattatccag attttcaatg
      541 gatttaatgc gctcgacgtt ggcgatactc atggcatttt tatcccatgc gtcaacatca
      601 taaccattgg ctgccaggta aagactgtta cgaccattgc cacagcccag atccagcgtt
      661 ttacccggtt taaccacttt caccgcttcc agtacttcag agtgtgtgcg ggttaattca
      721 tatttatcag taaaatagtt ttcgtcacga atgatcattc tttgtcctct gctttcatta
      781 aaacggcgcg ctcggttctg actaacaatt ttccctgcat cagaagcgca aaagtacgga
      841 tgagcagtat tgcaataata aaattggtaa agataaacag cggtaccgcc agcgtatgga
      901 aaaatccatt ttcgctgcca ctccccagat gcaaaccggt ggttgccagt gcagatacgc
      961 cgaacgagaa actccagaat gaagcattaa atggctggga gagataccat ggcatcagac
     1021 gtagcataaa cagcagttgc agcagtccat aaccaaaaag cattttcgcc agcgtatcac
     1081 cctcgccgcc gttgacgctc aaccaagcac tacaggccac cagcgcagga gcgagctgaa
     1141 tgccgagtga tgtccgcagt gccgtgggta attctcccga actgcgcaga cgctgcaaga
     1201 tcacaggttc aaggcttagc catgagaaaa cgcctgcgcc taaaaacacc agaccggcgt
     1261 cggtgtagcc caacgcacca caggccattg cgctgataaa gttgttggca actgtcggca
     1321 gatacagtcc aggcgtggta gcttcttcag ggtgagatcc gcgccataat cccgcagttt
     1381 gccaggcggc ataagccaac tgaaccacga caccaaaact gaacaggcac accgccagtg
     1441 ggcgaaacca cggaacaaaa ccaatcgcca ccagcatcgt cgttgccgga aacaaactca
     1501 caaaactgct catcactgga tggcgcactt ctgccagcac gctatgcgga aagcgtatga
     1561 gtcgggtaat aaatgcgcta gttaacaatc cccagatgat catcgccaga atcaccagcc
     1621 catcccctaa ccagtggctg acctgccaaa cctggctggc atagcgccag gcaaatccca
     1681 tccctatcgt ccccaacaca ataccaaagt agcctgccgg caaattgagc actttatcgc
//
LOCUS       DAPGWD010000314         1718 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPGWD010000314 DAPGWD010000000
VERSION     DAPGWD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.317"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1205
                     /locus_tag="RHX88_004501"
     CDS             <1..1205
                     /locus_tag="RHX88_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV2233088.1"
                     /translation="RVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSR
                     IQTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDR
                     LESEILADRVSEESRRWLASCGLTVEQIQNQMDPVYTPARKIHLYHCDHRGLPLALVS
                     TEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRY
                     ITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLP
                     DIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPG
                     MDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQ
                     RTSRGIRK"
     gene            1202..>1718
                     /locus_tag="RHX88_004502"
     CDS             1202..>1718
                     /locus_tag="RHX88_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233089.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNES"
BASE COUNT          503 a          384 c          454 g          377 t
ORIGIN      
        1 gcagggtggc aaaacgggta tggcgacgtg aacgggacct gacgggctgg atgtcgctgt
       61 cacggaaacc gcaagtgacc tggtacggct gggacggcga ccggctgacc acgatacaga
      121 acgacaggag ccgcatccag acgatttatc agccggggag cttcacgcca ctcatcaggg
      181 ttgaaaccgc caccggtgag caggcgaaaa cgcagcgccg cagcctggcg gatacccttc
      241 agcagtccgg cggcgaagac ggtggcagtg tggtgttccc gccggtgctg gtgcagatgc
      301 tcgaccggct ggaaagtgaa atcctggctg accgggtgag tgaggaaagc cgccgctggc
      361 tggcatcgtg cggcctgacg gtggagcaga tacaaaacca gatggacccg gtgtacacgc
      421 cggcgcgaaa aatccacctg taccactgcg accatcgcgg cctgccgctg gcgcttgtca
      481 gcacggaagg ggcaacagaa tggtgcgcag aatacgatga atggggcaac ctgctgaatg
      541 aagagaaccc gcatcagctg cagcagctta tccgcctgcc ggggcagcag tatgatgagg
      601 agtccggcct gtattacaac cgccaccgct attatgaccc gctgcagggg aggtatatca
      661 ctcaggatcc gattgggctg aaggggggat ggaattttta tcagtatccg ctgaatccgg
      721 ttcagtatat agattcaatg ggactggcat caaaatatgg acacttaaat aatggcggat
      781 atggagcgag acccaacaaa ccgcctacgc ccgatccaag taaattgccg gacatagcga
      841 aacaattaag actgccatat cctattgacc aggccagtag tgcgcctaat gttttcaaaa
      901 cattcttcag agcattaagc ccttacgact acacactgta ttgcaggaag tgggtaaaac
      961 caaatctgac ttgtacgcca caggatgatt cccagtatcc agggatggat acaaagacag
     1021 caagtgatta cctgccacag acaaattggc caacaactca attaccacca ggatatactt
     1081 gtgcagaacc ctatttattc ccagacatta ataaacccga tgggccagca acagcaggga
     1141 tagatgattt gggtgaaatt ttagctaaga tgaaacagag aacatcgaga ggaataagaa
     1201 aatgaaaaga gttttgttct ttttgctgat gatatttgtt agttttggtg ttatagctga
     1261 ttgcgaaata caagctaaag atcatgattg ttttactatt ttcgctaagg gtactatttt
     1321 ttccgcgttt cctgtcttaa ataataaagc catgtggaga tggtatcaga atgaggacat
     1381 cggtgagtat tattggcaaa cagaactggg tacatgtaaa aataataaat tcacaccaag
     1441 cggggcgaga ctcttaattc gcgtcgggtc actacgcctt aatgaaaatc atgccatcaa
     1501 aggaacgttg caggagttga taaatacagc agaaaaaacg gcgtttctcg gcgatcggtt
     1561 tagaagttat ataagagcgg gtatctatca aaaaaagagc agtgatccag ttcagttgtt
     1621 ggcagtcctt gataattcaa tcatggtaaa atattttaaa gatgagaaac caacttatgc
     1681 tcgaatgaca gcacatctcc cgaacaaaaa tgagtctt
//
LOCUS       DAPGWD010000315         1713 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPGWD010000315 DAPGWD010000000
VERSION     DAPGWD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.319"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..803
                     /locus_tag="RHX88_004503"
     CDS             <1..803
                     /locus_tag="RHX88_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV2233090.1"
                     /translation="GRYNSIRRERPLTDDELMQFVPSVFSGDKPESRSERYTYIPTIN
                     IINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGNINGQEVPEIILLNSHDGS
                     SSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFDKVTDNME
                     AMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIIIPRRREDKQNDLWTTWQRVQ
                     ENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1100..1702)
                     /locus_tag="RHX88_004504"
     CDS             complement(1100..1702)
                     /locus_tag="RHX88_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDV2233091.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
BASE COUNT          454 a          364 c          404 g          491 t
ORIGIN      
        1 ttggtcggta taattccatc cgccgtgaac gtcctttaac ggatgatgaa ttaatgcagt
       61 tcgtgccttc ggtattttcc ggtgataaac ctgagtcccg gagtgaacgt tatacgtata
      121 ttccaacaat caatatcatc aataagttac gtgatgaagg tttccagcca ttctttgcct
      181 gtcagagtcg ggttcgtgat ttgggacgtc gcgaatacag taaacatatg ttacgtcttc
      241 gcagggaagg gaatattaac ggacaggaag ttcctgaaat tatcctgctt aattcacatg
      301 atggttcatc cagttatcag atgatccccg gaatttttcg ttttgtctgc acaaatggcc
      361 tggtgtgcgg aaataatttt ggcgaaatcc gcgttccaca taaaggtgat attgtcgggc
      421 aggttatcga gggagcgtat gaagtgctcg gtgtctttga taaggtcact gataatatgg
      481 aggcgatgaa agaaattcat cttaacagtg acgagcaaca tttatttggc agagctgcac
      541 tgatggtcag gtatgaagat gaaaataaaa cgccagtgac acctgaacag ataattattc
      601 cccgtcgtcg ggaagacaaa cagaacgatc tctggacaac ctggcagcgg gttcaggaga
      661 atatgataaa aggtggatta tcggggcgaa gtgcctccgg gaaaaatacc aggacaagag
      721 cgattacagg tattgatggt gatataagaa tcaacaaggc gttatgggtg attgccgaac
      781 agttcagaaa gtggaagtca tgataatata agtaaagccc cgaaaaaatt ttcggggctt
      841 tacttatatt atcatttttc ggcattgctg tgtcgtctga tatacctcaa tttcatcaac
      901 cggctttccg gttgtctgta aatattgtgc ccgcgaaaaa tggtctggca ggaggctgcg
      961 cagttaccgg gtatatataa gatgggttcc cgcttcgcgg ggcttcggcc agtcagtatt
     1021 gtcactcata tgatattttt gtgtgtggcc ttccatgccg cttttgcggc ataaccggta
     1081 tctgacaatg tctgtaagat taattgttct gcacgctgtt aatttccgga agtgatttct
     1141 tcgtggccgg aagccggata cctttactgc tttttatccc ggtataaatc cggtaaacat
     1201 ctgatgcgta ggcaagacgt ctctggttct ggcgttcggt tttcctgaat ccggcattgt
     1261 atgcaccaac ggcctcccag gtgacgcccc attttttaaa tgctattgcc agataataag
     1321 caccggtata aatgttcatg cagggatctg ttgtcagatg ttctggctta attccgtagc
     1381 gggccagttc gttaaaatgc tgggaatcta cctgcatcag tccgctgcca tatcccgtta
     1441 ccggattaat accgatggca ttaacccggt aacgggattc ttgccatgat attgctctca
     1501 gtaaatcagg atctattttg taatcccggc ctgcaagatc aaagcaatca gtggcctggc
     1561 agatctcatt tataaacatc aggcagatgg ctaacatcca ttttttcatt tttccacctc
     1621 tggtgacttt atccgtaaat aatttaaccc actccacaaa aaggctcaac aggttggtgg
     1681 ttctcaccac caaaagcacc acaccccacg caa
//
LOCUS       DAPGWD010000316         1686 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPGWD010000316 DAPGWD010000000
VERSION     DAPGWD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.320"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            370..909
                     /locus_tag="RHX88_004505"
     CDS             370..909
                     /locus_tag="RHX88_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV2233092.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSAQPQPEPQSEAGTKKGGAKTKGRGRKAAQPE
                     PQLQPPEGDDYGFSDDIPF"
     gene            972..1205
                     /locus_tag="RHX88_004506"
     CDS             972..1205
                     /locus_tag="RHX88_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV2233093.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1271..>1686
                     /locus_tag="RHX88_004507"
     CDS             1271..>1686
                     /locus_tag="RHX88_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDV2233094.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGILTADWPVRVKVIPQELATAASMTENGHRRDMHP"
BASE COUNT          397 a          451 c          510 g          328 t
ORIGIN      
        1 cgccgtttcg caaaccgcgc ttcacgtcgt tgcggcgggt ccctgcccgt tgttctgtcc
       61 ggtgccattc acctgtttaa gcccctgtcg ccgggggcat agacagccac aaccgtctcc
      121 gccagtccag gggtgaaatg tacatcccgc aaaacttttt gcccagaagc aggcaaaaac
      181 ttttgcggtc tgcccccgga cagtcgtttc cggccgtggc agtgatgccc catgcggcga
      241 aggttctcaa aaaggtgatg gtgaataccg gagggaacaa aaggcaggtc cccgcaaaac
      301 gtcaaaaccg cggctcccga aactcaaaag atgatggggg ctgaaaacat caacagaagg
      361 aggcacatca tggcagttcg tggcattaac aaggtcattc tcgtaggacg cctgggaaaa
      421 gatccggaag tccgttacat ccccaacggg ggcgcagtgg caaacctgca ggtcgccacg
      481 tcggaaacct ggcgtgacaa acagacaggg gagatgcggg agcagacaga atggcatcgc
      541 gtggtgctgt tcggcaagct cgcggaagtg gctggcgaat atctgcgcaa gggcgcgcag
      601 gtctacatcg aaggtcagct tcgcacccgt agctgggaag ataacggtat cacccgttac
      661 gtcaccgaaa ttcttgttaa gaccacaggc accatgcaga tgctgggacg tgccgcaggt
      721 gctcagactc agccggaaga ggggcaacag ttcagcgctc agcctcagcc ggaaccacag
      781 tcggaggccg gtacgaaaaa aggtggcgca aaaacgaaag gccgtggacg taaggccgcg
      841 cagccggagc ctcagctgca accgccggag ggtgacgatt acgggttttc agacgatatc
      901 ccgttctgat cgggctgact gtgataaccg ccccgccccg ttctgtgcgg ggcatcaccg
      961 gagagatgag aatgagcgaa tatttcagaa tacttcaggg gctgcctgac ggtcctttta
     1021 cccgcaaaca tgccgaagct gttgccgcac agtaccggaa cgtctttatc gaggatgacc
     1081 acggcgaaca gtttcgcctg gttgtccgta ataacggtgc aatggtctgg cgtacctgga
     1141 attttgagga cggtgccggg tactggatga accatgtcat cagggatttc gggattctta
     1201 agtaagagag gtgccggacg gggacacgtc cggcagacaa tcattattac cgttttggga
     1261 ggtactaact atgtcagtta cagagtctaa ggcaaaaacg gagcgtaaat ccagccgtaa
     1321 acctgcaaaa acgcaggaaa cagtcctgtc tgccctgctg gcgcagacgg aggaagtgag
     1381 cgtgccgctg gcctcactga ttaaatcacc gctgaatgtg cgcacggtgc cgtattctgt
     1441 ggagtccgtc agcgaactgg ctgagtccat taagggcgtt ggcctgttgc agaatctggt
     1501 cgtgcatacc ctgcctggtg accgttacgg tgtcgccgca ggtggtcgcc gactggctgc
     1561 actcaacatg ctggcagagc gtggcatcct tacggctgac tggcctgttc gcgtgaaggt
     1621 tattccgcag gaactggcga ctgccgcatc aatgactgaa aacggtcatc gtcgggatat
     1681 gcaccc
//
LOCUS       DAPGWD010000317         1672 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPGWD010000317 DAPGWD010000000
VERSION     DAPGWD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.321"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..698
                     /gene="zinT"
                     /locus_tag="RHX88_004508"
     CDS             48..698
                     /gene="zinT"
                     /locus_tag="RHX88_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDV2233095.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEAEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            1041..1571
                     /locus_tag="RHX88_004509"
     CDS             1041..1571
                     /locus_tag="RHX88_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV2233096.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKVLIKNIHETWANVGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          522 a          357 c          318 g          475 t
ORIGIN      
        1 caatataaca ttacacatca tatacaatga ctctggagga aactgttttg gcgattcgtc
       61 ttcacaaact ggctgttgct ttaggtgtct ttattgttag cgctcctgcc ttttcgcatg
      121 gtcatcactc acacggcaaa cccttaacag aggccgaaca aaaagctgct aatggtgttt
      181 ttgatgatgc caatgtacaa aaccgaacgc tcagtgactg ggatggagtc tggcaatccg
      241 tttatccttt actgcaaagt ggcaaacttg accccgtctt tcagaagaaa gcggatgcag
      301 ataaaactaa aacatttgct gaaattaaag attattatca caaaggttat gcaacagata
      361 tcgagatgat tggcattgag gacggcattg ttgaattcca tagaaataat gaaacaacat
      421 cctgtaaata tgattacgat ggatacaaaa tactcaccta taaatcaggc aagaaaggcg
      481 ttcgttactt attcgaatgt aaagatcctg aaagcaaagc ccctaaatat atacaattta
      541 gcgatcatat aattgcacca cgaaaatcat ctcattttca catttttatg ggtaatgatt
      601 cacagcaatc tttattaaat gaaatggaaa actggccaac gtattatcca tatcagttga
      661 gtagcgaaga agtggtcgag gaaatgatgt ctcattgagt attctcatga taacgcctcg
      721 atgccgcttt agtaagtcat cataactgcc actggtccat ccacaaacgc cgctgaacgc
      781 aagctagcta cagacacgct catcactatg acgtgtctgt atattaataa gctaacccgc
      841 attgagttaa ccaataacgg attccataca caatacggcc aacagagaaa gatacctgtg
      901 ctcacgccat tgcttatatt ggctggttac aatgtgcact atcaattttt taaataacaa
      961 attcttaaaa ccacatctgt ataaggaatt tttaaggttc gtgggatagc ttgactgtga
     1021 aaattacagg agctacaaaa atgaaccgat tctcaaaaac tcaaatttat ttacattgga
     1081 taacgctgct tttcgttgca ataacctatg ccgcgatgga actccgtggc tggtttccta
     1141 aaggtagtag tacttatctg ctgatgcgag aaacacatta caatgcgggt atattcgttt
     1201 gggtgttaat gttttcacgc ctgattataa aacaccgtta tagtgatcct tctattgtgc
     1261 caccgccacc tgcctggcaa atgaaagcgg cttcgctaat gcacatcatg ctttatataa
     1321 ccttccttgc acttcctctg ctggggattg ctttgatggc ttacagtgga aaatcgtgga
     1381 gtttccttgg tttcaatgtg tctccctttg ttaccccaaa cagcgaaatt aaagtactga
     1441 taaaaaatat tcacgaaacc tgggcgaatg taggttactt tttaatcgca gctcacgctg
     1501 gcgcagcact ctttcatcac tacattcaga aagataatac tctgttacga atgatgcctc
     1561 gccgcaaata agtcgatccc ctgacaagga aatgtgcact atggacatca acgcgtcata
     1621 aatgtttacg caagtgcaca atacaccctt tccactatca acaaggagga gc
//
LOCUS       DAPGWD010000318         1652 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPGWD010000318 DAPGWD010000000
VERSION     DAPGWD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.322"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..1277
                     /gene="tet(B)"
                     /locus_tag="RHX88_004510"
     CDS             72..1277
                     /gene="tet(B)"
                     /locus_tag="RHX88_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDV2233097.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLNNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1390..>1652)
                     /locus_tag="RHX88_004511"
     CDS             complement(1390..>1652)
                     /locus_tag="RHX88_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2233098.1"
                     /translation="EVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVL
                     KNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          410 a          306 c          366 g          570 t
ORIGIN      
        1 taatttttgt tgacactcta tcattgatag agttatttta ccactcccta tcagtgatag
       61 agaaaagtga aatgaatagt tcgacaaaga tcgcattggt aattacgtta ctcgatgcca
      121 tggggattgg ccttatcatg ccagtcttgc caacgttatt acgtgaattt attgcttcgg
      181 aagatatcgc taaccacttt ggcgtattgc ttgcacttta tgcgttaatg caggttatct
      241 ttgctccttg gcttggaaaa atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt
      301 cattaatagg cgcatcgctg gattacttat tgctggcttt ttcaagtgcg ctttggatgc
      361 tgtatttagg ccgtttgctt tcagggatca caggagctac tggggctgtc gcggcatcgg
      421 tcattgccga taccacctca gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa
      481 gttttgggct tggtttaata gcggggccta ttattggtgg ttttgcagga gagatttcac
      541 cgcatagtcc cttttttatc gctgcgttgc taaatattgt cgctttcctt gtggttatgt
      601 tttggttccg tgaaaccaaa aatacacgtg ataatacaga taccgaagta ggggttgaga
      661 cgcaatcgaa ttcggtatac atcactttat ttaaaacgat gcccattttg ttgattattt
      721 atttttcagc gcaattgata ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa
      781 atcgttttgg atggaatagc atgatggttg gcttttcatt agcgggtctt ggtcttttac
      841 actcagtatt ccaagccttt gtggcaggaa gaatagccac taaatggggc gaaaaaacgg
      901 cagtactgct cggatttatt gcagatagta gtgcatttgc ctttttagcg tttatatctg
      961 aaggttggtt agttttccct gttttaattt tattggctgg tggtgggatc gctttacctg
     1021 cattacaggg agtgatgtct atccaaacaa agagtcatca gcaaggtgct ttacagggat
     1081 tattggtgag ccttaacaat gcaaccggtg ttattggccc attactgttt gctgttattt
     1141 ataatcattc actaccaatt tgggatggct ggatttggat tattggttta gcgttttact
     1201 gtattattat cctgctatcg atgaccttca tgttaacccc tcaagctcag gggagtaaac
     1261 aggagacaag tgcttagtta tttcgtcacc aaatgatgtt attccgcgaa atataatgac
     1321 cctcttgata acccaagagg gcatttttta cgataaagaa gatttagctt caaataaaac
     1381 ctatctattt tatttatctt tcaagctcaa taaaaagccg cggtaaatag caataaattg
     1441 gcctttttta tcggcaagct cttttaggtt tttcgcatgt attgcgatat gcataaacca
     1501 gccattgagt aagtttttaa gcacatcatc atcataagct ttaagttggt tctcttggat
     1561 caatttgctg acaatggcgt ttaccttacc agtaatgtat tcaaggctaa ttttttcaag
     1621 ttcattccaa ccaatgatag gcatcacttc tt
//
LOCUS       DAPGWD010000319         1650 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPGWD010000319 DAPGWD010000000
VERSION     DAPGWD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.323"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            971..1573
                     /gene="fimB"
                     /locus_tag="RHX88_004512"
     CDS             971..1573
                     /gene="fimB"
                     /locus_tag="RHX88_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDV2233099.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          499 a          285 c          357 g          509 t
ORIGIN      
        1 ttcgtgtcac aaaatatgtc gatatttttc taagaagaag atgccatgtg aagccagacg
       61 aacacttgcg gaggccttca caaaccaaag attgttgttt aattatctgt tttataagta
      121 atttatttga agtataaaca aggaaagatt tgcaagctaa ctcagtgagc gtggtgaaaa
      181 tcagtgttta cccgccatca ggctgagcat aattctcatc atgaaatatg tttcctggtt
      241 tgtggcttgt aactggtcac ttctgaagtc gatctggaga ggcttgttga tgttggtgtt
      301 ttcaggatga tgtttcactt agtttgtttg ccgtatcgcc cggcgaatgg ctgtgattga
      361 ggaaggttta agtcgtagtg accaaagcta tatttaccaa cgaatgtaga tgaaaaaatc
      421 atctcctgcg ttccccatat ctccaggata aaaaggaatg taacaatctc atggcgtaag
      481 ctgacgaatc agccaggaat aatcgctagg gacctaagaa ttagcatgat aatagccact
      541 aagaaattac tgcgctccat gaaatagcca ttttgtggca aatggagttg actaataatg
      601 tcatatgtga gacggctagt tgaacgaata ttaaattttg ctgaattttt tatgttgatt
      661 ttacttgtta cagaacatat cacatgatat atagataaga ttagttgcat taatgatgag
      721 ggttattatt agattcgtat ccgattgata aatatataaa ggtacatagc atgcaagagc
      781 atggcgtttg tatggcaacg ttattataat taactgttgc tactccattt aagttcactc
      841 agaagaactg gtccacttac gttagttatt aagcaaacgt tcgcttttat aaacataatc
      901 aggataaaaa tgttggatta ttgctaaccc agcacagcta gtgcgcgtct gtaattataa
      961 gggaaaaacg atgaagaata aggctgataa caaaaaaagg aacttcctga cccatagtga
     1021 aatcgaatca ctccttaaag cagcaaatac cgggcctcat gcagcacgta attattgtct
     1081 gactttgctt tgttttattc atggtttccg ggcgagtgaa atttgtcgat tgaggatttc
     1141 ggatattgat cttaaggcaa agtgtatata tatccatcga ttaaaaaaag gcttttcaac
     1201 aacgcatccg ctattgaaca aagaagttca ggctttaaaa aactggttga gtatccgtac
     1261 ttcgtacccg catgctgaga gcgagtgggt atttttatct cgtaagggaa atccgctttc
     1321 tcggcaacag ttttaccata ttatctcgac ttccggtggt aatgccgggt tgtcactgga
     1381 gattcatccg cacatgttac gccattcgtg tggttttgct ttggcgaata tgggaataga
     1441 tacgcgactt atccaggatt atcttgggca tcgcaatatt cgtcatactg tctggtatac
     1501 cgccagcaat gcagggcgtt tttacggcat ctgggataga gccagaggac gacagcgtca
     1561 cgctgtttta tagagatagt tgagatacca gggatggtgt taaaaactac attattctta
     1621 cgatatatca aaaatgatta accatttatt
//
LOCUS       DAPGWD010000320         1611 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPGWD010000320 DAPGWD010000000
VERSION     DAPGWD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.324"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1365)
                     /gene="ccp"
                     /locus_tag="RHX88_004513"
     CDS             complement(<1..1365)
                     /gene="ccp"
                     /locus_tag="RHX88_004513"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDV2233100.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDTQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNG"
BASE COUNT          426 a          425 c          339 g          421 t
ORIGIN      
        1 cccgttcaga ctgtgcagga aagctacgat atcatccaca tcctcctgcg gcagctcttt
       61 gcctacctga tagcgcaaca tcagtttcac cgccccgtcc agcgtcggca cgtcaccgcg
      121 atggaagtac ggtgccgtta aagcaacgtt acgtaaaccg ggtactttct gacgcaattt
      181 atcacgctct tctttagtca cattcatacg accaatatcc gccgccgtaa tttccccaaa
      241 gttaaagtct tttttcagcc ccaacggttc gaaggaacgt ccgccgagaa taattccacc
      301 atgacaagtt gcacatttat tatctttaaa taattgatag ccttttttct gttgcgccgt
      361 cagcgcattt tcatccccac gcaaccattt atcaaatggg gaatccggcg taattaatgt
      421 tttctcaaat tcagcaatgg catcagtaat attttcgcca ctgaaacctt gcggatagac
      481 gtcgaggaac tgcgctttaa gctgcggatc tttttccagc ttagcaataa tttcgtccca
      541 ggattttgac gccatttcaa tcgggttcaa cggcggtcca ccagcctgat cctgcaatgt
      601 tgccgcacga ccatcccaga actgctcaac gttaaatact gagttaaata ccgtcggcgc
      661 gttaatcggc ccaactgcgc caccaacacc aatcgatgtt tttctgccat cgacgccccc
      721 cgcattcaac gcatggcaat gagcgcatga aatggtgcta tcagccgata aacggggatc
      781 gtgatacagc gcaaagccca acgccacttt ttgcgtatcg gtaggcagtt tttgcgggat
      841 gggctgcacc ggttcattgc gatgctccgg agcagtatca ttgctggcgt aatattccgc
      901 gcgctgtttt gcaatccagg ccagtatttc cgcccgctct tcatcactca ccttacccgc
      961 ccagtgaagc gcggtataac gcgttggtgg catagtttca tactgcatca cccattcaat
     1021 cttattcaga tcgctttgcg aaacgggttt atcagccagc agtgccgcac gcacggcctc
     1081 gaggttaaaa gatttatatc caagcttaat gtcgtaatcc atcaactgtt tcgcgccagg
     1141 aatataataa taggcgggta attctgccga aggcgtgtgg caatagtcgc atcctttttc
     1201 gcggagaaag cctaaaacct tattattttc actgacagca gatgcctgaa catcggcctg
     1261 tttactgcgt ttattatcgt ggtaccacac ataaccagat aaccctaaat agcaaatcgc
     1321 gacgccagcc aggccgatcg cggtaatacg tgagaccatt ttcattattc ttccctcacg
     1381 gtggtgagtt attgtgaccc agaaaatttt ccagaatgct gcaaacgtct ctgcgagaca
     1441 aagccatcat aaaaaacagg gagatacgct ttttgatagg aatcaatcaa ttacatgaat
     1501 ataaactatc aataagatag cctgtgccta ttacccagat caggaatggc atatcatgat
     1561 tgcggcgaga gcaggattga atgttctgaa aatgaaaaac aacacgaaat c
//
LOCUS       DAPGWD010000321         1596 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPGWD010000321 DAPGWD010000000
VERSION     DAPGWD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.325"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..581
                     /gene="tetR(B)"
                     /locus_tag="RHX88_004514"
     CDS             <1..581
                     /gene="tetR(B)"
                     /locus_tag="RHX88_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HDV2233101.1"
                     /translation="LLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEM
                     LDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQL
                     AFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIE
                     LFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(559..1245)
                     /locus_tag="RHX88_004515"
     CDS             complement(559..1245)
                     /locus_tag="RHX88_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV2233102.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1253..>1596)
                     /locus_tag="RHX88_004516"
     CDS             complement(1253..>1596)
                     /locus_tag="RHX88_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HDV2233103.1"
                     /translation="ALLGKTLGNKGIGLEGGGIFTVGDECHAHFLVEQGKEAKIALEQ
                     AGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYSDHANQLILITDNDSM
                     AASVTLPWAIK"
BASE COUNT          475 a          355 c          305 g          461 t
ORIGIN      
        1 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
       61 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
      121 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
      181 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
      241 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
      301 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
      361 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
      421 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
      481 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
      541 gcggattaga aaaacaactt aaatgtgaaa gtgggtctta aaagcagcat aacctttttc
      601 cgtgatggta acttcacggt aaccaagatg tcgagttaac caccctttag attcataaag
      661 cgaaaataat gcggctccaa cgtacccacc taaatggaaa cggcgttcac tccaatctaa
      721 acacgcacaa cagattttac gtgaatgttt ggaaggaacg tcaattccca tttcatgaaa
      781 atattgaata ccacttaatg tgatcattga accattttca gtgatccatt gctgttgaca
      841 aagggaatca tagatcttaa cggcaacttc gccagctaaa tgatcatagc aagtacgtgc
      901 ttttcgtaaa tgcactggcg tggaaacttt ggcatgtacg ccatggttta aggagatccc
      961 catcatactt tccatcaatt cagcaatatc ttttcctgct agccgaaaat aacgatgctt
     1021 gccttgagct actactgtga ttagctggca atctaataat ttagataaat gactgctcgc
     1081 cgttgaagct gatatattcg ccacagaact tagctcagtg gccgtccaag ctcgcccatc
     1141 catcaaagca ctgagtattt taactcgtga aatgtcagac atagccgccc ctatcgcggc
     1201 tattgaggac tcaaaggtaa cctcttttcg tattaaatta gccatcgcaa gttcacttta
     1261 ttgcccaagg gagcgtaaca gatgcagcca tactatcatt gtccgttatt aatatcagtt
     1321 ggttagcatg gtcactgtat tgcactaaaa tattaatgtt attctccgcc aatactcgtg
     1381 ctatttcgcc aagttccccc ggtttttcct gttttaactt acgaattaat ggtgtccgga
     1441 tcgcaagtac taacagtcca gcttgctcta gcgctatttt agcttccttt ccttgttcaa
     1501 caagaaaatg agcatggcat tcatcaccaa ccgtaaatat ccctccccct tccaatccaa
     1561 tacctttatt acctaatgtt tttcctagta atgcta
//
LOCUS       DAPGWD010000322         1543 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPGWD010000322 DAPGWD010000000
VERSION     DAPGWD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.326"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..962)
                     /locus_tag="RHX88_004517"
     CDS             complement(<1..962)
                     /locus_tag="RHX88_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV2233104.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKSRHLKRNGNIA"
     gene            complement(988..>1543)
                     /gene="rfaY"
                     /locus_tag="RHX88_004518"
     CDS             complement(988..>1543)
                     /gene="rfaY"
                     /locus_tag="RHX88_004518"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDV2233105.1"
                     /translation="VSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETD
                     RVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASM
                     EKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIVNEIK
                     DYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          482 a          325 c          263 g          473 t
ORIGIN      
        1 gcaatattac catttctttt caaatgtctg gatttcactt gatactgctt ttcattcgtg
       61 gctggaataa acgccacatc attccagcaa gaagcctgat aagcctcatt aaaatacttc
      121 tggcagggat aattcgtcca gtcgttccag ggcttcgtta cgccaacata atgaatcagc
      181 ttagtatcat cggtaatggt ctttttataa tcttcatcgc ttttattttt taactcatag
      241 tcaatgccat aaaagcaatc ataatcgccg ctaataaaaa tacaatgacc aaagaacaga
      301 atatttaaaa tatcctgatc aaggcattta tacttcgtgt gctgcgggtc acttgccagc
      361 atagcaatgg ctttattcat aaaatcattt ttcgcccatg cttcaagctg cagatatact
      421 acaccggcgt tgaaataatt ccctggaagt ccctctatag ccagacgctt cgctggtttt
      481 tcctgcataa aaggcacatc tttaataaca gctgcgaact tatcctcatc aaaattaata
      541 tcaagcaatc cagctaatga gcctttacag atgacatcag catcgatata taacagattt
      601 tcaatagttt cactcaaata ctggaacgca actaaacgga agaatgttgc ataagaccac
      661 tgtttcaggc tggggaagat atcaataaag gcactggaga ctttataaat tattacttca
      721 acgttagcat ttttgctggt aaaagcatta acacgttgta gataatcttc atcaatgtag
      781 tcggtaaaga gatgaaactt aaccgcaagg tccgggttat gcataacaac tgattgcata
      841 gatactgccg caccaaataa aaagttacgg tcaataccgt aagcgatatt taacacacga
      901 tcggtgttag caatttcccc agagacagcg acttcagtaa tactctcagc taataaatcc
      961 atgttggttc cgttttgact gtgtggttta cgctttgcct tttaattttt taataaattt
     1021 gcgtagtttg gttctgtaga ttactgaata gtagccataa tcttttattt cgttgacaat
     1081 acccaggtga cgttccatgg ccagacgatc acgagcttta cgctctgccg tacagctttt
     1141 accggatagg tcaattatcc tgacaccatc tttactaacg ataaagttac cccggtgtgg
     1201 gtcgcccgat agcatattta aggcatgcaa tttttccatt gaagccttaa tttctgcttt
     1261 aacattttct ggaataatcg gcatatcatt gagctctacg ccctcgacat actcgataag
     1321 catgataaaa acactggcat agttgaaaat tttacgctca gccagaaaat aaaaatcatt
     1381 aggaaaagta agacctgcac ttctgacacg atcggtttca acgatcaaat tctgatagta
     1441 gtcacctttt acaaaagatt ttaaaaaacg ttcattcctt ttcgttttcg gagcaaagac
     1501 tttaaaaaca tagcgaccat atttcgtatc aatcaaagat act
//
LOCUS       DAPGWD010000323         1453 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPGWD010000323 DAPGWD010000000
VERSION     DAPGWD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.327"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            242..1273
                     /locus_tag="RHX88_004519"
                     /pseudo
     CDS             242..1273
                     /locus_tag="RHX88_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          404 a          342 c          352 g          355 t
ORIGIN      
        1 gtaccccatc aatctgcata taaaattgct ggccacgcta catcctgtta gcgaaagtac
       61 tgattagtac ttcaatggcc agtgcaaaac tgaaaatcca attcaggcaa ataagggatg
      121 atgataccgt aagtaaatcg tgatatcgcg ttaaatatat tgccacatcc acacacgttg
      181 agtttgctta ttgtgcactg atttctatga tgtaccctga tcttagtcag aggtgactca
      241 catgacaaaa acagtatcaa ccagtaaaaa aaccgtaaac agcattcgcc tgaatttcgc
      301 agtgaagcac taaagcttgc ggaacgcatc ggtgttgctg ccgcaggccg tggactcagc
      361 ttgtatgaat cacagctcta caactggcgc agtaaacaga aaaatcagca gacttcctct
      421 gaacgtgagc tgccggaaca ggatgaagag ctggccatac tccaaaaggt agcgacatac
      481 ttcgcgaaac gccagaaatg aagtatgtct ttattgaaaa acatcaggct gagttcagca
      541 tacaggcaat gtgccgcgtg cccgcagcgg cgggtatatg tggtatcggc ggcgggcaag
      601 gataaaagca cacgtcagca gttccgtcag tactgcaaca gcgttgtcca cgctgttttt
      661 accctgtcaa aaccgcgtta cggtgcacta agcctgcgcc gttagggact gagggcaaaa
      721 gcttcccgga agttcagcct ggtcagtcac cgcgcacaca gcctgcctgt gtcagaaaat
      781 ctgcttgagc aggattttta cgctgttggc ccgaaccaga agtggtcggg agacatcacg
      841 tacttacgta cgtgatgaag gctggcagta tctggcagtg gtcattgacc tggggtcacg
      901 tgccgctatt ggctggtcaa tgtcaccacg catgacagcg caaatagcct gcgatgccct
      961 gcagatggag ctgtagcggc gtaagagacc acagaacgat atcattcgga ggccagtact
     1021 gctctgcaga ttatcaggca ttactgaagc ggcataatct gcgtggcagt atgatcgcaa
     1081 aagactgctg tttcgatcat gcctgcgagg aaatcttctt tcattcactg aaagtggaat
     1141 gtatccacgg agaactcttt attagctggg aaacaatatg ggcaacagtg tttagtgttt
     1201 acaatcgctg gcgacggcac agtggatgtg gcgccttagt cctgaacaat ttgaaaacca
     1261 gaacctcgct taggcctgtg tccatattac gtgggtagga tcagatcacc cctcctgacc
     1321 atgagtggga ggaaggaaac agaactcatt taacgcactt gcaccacctt catatcaaaa
     1381 aatacacttt tccaacgaaa cttagtgtta agtttatgtt gccttttttc tgtcacctct
     1441 tttaatttaa ttg
//
LOCUS       DAPGWD010000324         1445 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPGWD010000324 DAPGWD010000000
VERSION     DAPGWD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.328"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(55..459)
                     /locus_tag="RHX88_004520"
     CDS             complement(55..459)
                     /locus_tag="RHX88_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDV2233106.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(680..1411)
                     /gene="bluR"
                     /locus_tag="RHX88_004521"
     CDS             complement(680..1411)
                     /gene="bluR"
                     /locus_tag="RHX88_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDV2233107.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYTAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          375 a          325 c          316 g          429 t
ORIGIN      
        1 attatgctaa tgccttactg aattatgaag catttcttaa gcatccaact ttagctagat
       61 taatggttta ttattttcta catcttcaat atataaaagc gtattatcaa tggcgtagta
      121 actgcgtttg ttatgattaa catcagtaac ccaccggaaa acgcccgcgc ctgctagtgt
      181 tgaacagtat tcccgaaatg tagattttcc gcaaatatga agcaatgcgg cctcttttat
      241 tttagcaggg ttcttggtcg tactaacttt taacaggttc ctggttcctc ttaataacaa
      301 aaccgtgtca tcgtgagtaa taattctgat gttatccgta gccagataat aaatgtaatg
      361 tgcaatacgg tgatgtttta attctgaata aaaccaggag aagttttgct cttttctcac
      421 ttgctcaaac atcttttgaa aaacaacgac ctgatccatc aggataataa cctcttgtta
      481 gttgtgagac tgcgtagtgt gcacgatcgg ttttaccact tcaatctggt ctgtcctttg
      541 gctgtgatat gtacagagtg tgatagaggg aatatctgaa ttctcccggt gagcattttg
      601 caacggacca gctccggtac aaacgctgtt gtgggttcag attataacat tctgtctaag
      661 gggcgggata aaggtgaaat tagggggcat gaaagatgac tttataacct tgctcacccc
      721 agtgttgtaa aagttcgttt tgccttctcg ttggtgccat gcctgtccag acaatcaatg
      781 tttgcgtcgg gaacagttcc gggcgcggcg attcaatggg atcagcaaga acagaaatgt
      841 gccatcctga caatgataaa cgccatgctt ccagccacaa tcgtgctctc tcttcaacat
      901 tccacgccat cagaatggct tctttaccgg gcttacggcg catttcgaaa agcgaggctg
      961 ctgtgtattc aattaatgcg ccgtcaaaca tactgctcat aatgcgggag gtgttgtgat
     1021 caagcacgag acgctggcga acaggaaggt aaacatgatt aatcaattga tctactgggt
     1081 actctcgacc cagtgaaata attctcgcgc gtagtttggc aggattagcc atgcgaagaa
     1141 ttgacatcat ctcttcttgc aggcggctcc agtcatcttc cgtatcctgg ctggtggttt
     1201 ccagtaatgc tttaactttg cctacaggga cgccattact tatccaacgc ttgatctctt
     1261 cgatgcgttg tatgtcttct tcatcaaaga gtcggtgtcc gccttcactg cgctgtggtt
     1321 ttaacaaacc gtagcggcgt tgccaggccc ggagagtgac aggattaatc ccgcaacgtt
     1381 cagcaacatc accaatgctg taataagcca caattcctcc ttgcggtcac aaatctccgt
     1441 cgcct
//
LOCUS       DAPGWD010000325         1441 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPGWD010000325 DAPGWD010000000
VERSION     DAPGWD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.329"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            111..821
                     /locus_tag="RHX88_004522"
     CDS             111..821
                     /locus_tag="RHX88_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2233108.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            863..>1441
                     /locus_tag="RHX88_004523"
     CDS             863..>1441
                     /locus_tag="RHX88_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HDV2233109.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSF"
BASE COUNT          411 a          332 c          302 g          396 t
ORIGIN      
        1 cgcattaagt ctggaaaccc ggcgactcgc caatgtatta gcaacattaa tcgattgtct
       61 gatgtaacgg ttttcccgct tatcctctta acacatggag aatgcatcag atgcaaacta
      121 cgcgtacccc ctactctatt tcctttatgg cgacagtgtt acttttattg ctctttgctt
      181 gccatagcac cgttgccaac gcagctgtcg cactgggtgc gacgcgggta atttatccgg
      241 caaaccagaa acaggtttta ttgcctgtca cgaataatga tcctgccagt gtctatctca
      301 ttcagtcatg gatagaaaat gcaggtgacc aaaaagacac ccagtttgtc attaccccac
      361 cgcttttttc catgcaaggt aaaaaagaga atacgctgcg gataatcaac gctactaatc
      421 atcagttacc aggcgaccgt gaaagcctgt tctgggtgaa tgttaaagct attcctgcta
      481 tggaaaaaga ccagaagaat gagaatacat tacagctggc aattattagc cgcataaaaa
      541 tgttttatcg ccccacgaac ctggcaatgg cacctgaaga agcgcctgca atgcttcggt
      601 ttcgtcgcag cggtagcaaa ctcacgctta ttaatccaac gccctatttc attaccgtaa
      661 caaatatgaa agcaggaaat agcaatctcc caaacaccat ggtgccgcca aaaggtgaag
      721 tatcggtgga tatcacgcat gctgcaaccg gggatatcag ttttcagacg atcaatgatt
      781 acggtgcatt aactccgcgt attaaggcaa ccatgcagta agccagtgac ctaattaata
      841 ataaactcaa tcggaattaa taatgacagc cattcgtgcc gcatttaagg catatcgtat
      901 gcatcaggtg ttaataatgc ctcgctttgc acggcttacc attgcccttg gcttagcaac
      961 tgcggtattt cccgttgacg cagagtatta ttttaatcca cggtttttaa gcaatgatct
     1021 tgctgaatct gtcgatttat ccgcctttac taagggacgc gaggcaccac ctggtacata
     1081 tcgggtcgac atttatttga acgatgaatt tatgactagt cgggatatta cttttattgc
     1141 agatgataat aatgcagaac tcatcccatg cctgagtaca gatcttcttg tcagtcttgg
     1201 tataaaaaaa tcagcgttat tagacaataa agaacattcg gcagaaaaac atgtgccgga
     1261 caacagcgca tgcacaccac ttcaggatcg tttggccgac gcatcaactg agtttgatgt
     1321 tggtcagcaa catttgtcac tctccgttcc gcaaatttat gttggcagaa tggctcgcgg
     1381 ctatgtttct cctgatctgt gggaagaagg aataaatgct gggcttctaa actatagttt
     1441 t
//
LOCUS       DAPGWD010000326         1436 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPGWD010000326 DAPGWD010000000
VERSION     DAPGWD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.330"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            54..653
                     /locus_tag="RHX88_004524"
     CDS             54..653
                     /locus_tag="RHX88_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDV2233110.1"
                     /translation="MTMSSDEITIQKEAIINSDVILLQLETNYTALQQAITLAQKNSI
                     PVIINPAPYNDIVNELIQDVDYITPNETEAGLLSGIDVHDLESAKRAAEAIHNKGVKN
                     TVITLGSKGSLAFDGKKFIHSPAFPAVVKNTAGAGDEFNGSLASELAKGKSLESALCY
                     ASAFASLAVETSNASDMPEHESVIHRIQSIHYQQTIFTH"
     gene            667..1425
                     /locus_tag="RHX88_004525"
     CDS             667..1425
                     /locus_tag="RHX88_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDV2233111.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNAPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
BASE COUNT          452 a          292 c          303 g          389 t
ORIGIN      
        1 ttgttaatga aggtactggc gataatgtta ttgcgatcta tcccggtgcc aacatgacaa
       61 tgtcgtccga tgaaataact attcagaaag aagcgattat taattctgat gtcatacttc
      121 tgcagctcga aacaaattat accgctctgc aacaagcgat cacattggcg caaaaaaaca
      181 gtatcccggt cattattaac ccggcaccct ataatgatat cgtcaacgaa cttattcagg
      241 atgttgatta tatcaccccc aatgaaacag aagctggttt actgtccggc atagacgtac
      301 acgatcttga atcagctaaa cgagctgcag aagctatcca caataaaggg gtaaaaaaca
      361 cggttatcac gctcggtagc aagggctcgc tcgcatttga tggcaaaaaa ttcattcatt
      421 cgccagcgtt tccagcggtc gttaaaaata cggcaggagc cggggatgag tttaacggct
      481 ctctggcatc agaacttgcg aaaggaaaat cactggaatc agcactgtgt tatgccagcg
      541 cctttgcatc cctagcggtg gagacaagca atgcttccga catgcctgaa catgaatctg
      601 tgatacatcg aattcagagt atacattatc agcaaactat ttttacgcat taagaaggga
      661 cagagaatga ataacaccaa aaaatcacta aaagtgctct ttattgggga atcctggcat
      721 attcatatga ttcattcaaa aggatatgac agttttacct caagtaaata tgaagaaggt
      781 gcgacctggt tactccagtg tctgaaaaat agtcaggtag atgtcaccta tatgccagca
      841 catacggtac aaattgcctt ccctgaagat gtggcacagt tagaacaata tgatgccatc
      901 gttattagtg atattggtag taatactttc ctgctacaaa atgatacttt ttatcagctt
      961 cgcataaaac caaacgctct cgaactgatt aaagaatatg tgaataacgg tggtgggtta
     1021 ttgatgattg gcggctatct ctcattcatg ggaattgaag caaaagccaa ttataaaaat
     1081 acagttctgg ctgatgtgct cccggttacg atgttagatg gtgacgatcg tgtcgaaaaa
     1141 ccagagggtg ttattgcaca gccatcacaa cctgaacatc cagttattaa agggttctct
     1201 gaatatcctt ttttcctagg ttataaccgg gcgatcgcta aagaaaatgc tgaggtggta
     1261 ttaacaataa acaacgctcc attactggtc tttggtaact atcacaacgg gaaaatagcc
     1321 tgctttatga gtgactgttc accgcactgg ggtacacagc agtttatgtc ctggccgttc
     1381 tataccgcgt tgtgggtaaa tatactgact catattgcaa gataataata ataatg
//
LOCUS       DAPGWD010000327         1428 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPGWD010000327 DAPGWD010000000
VERSION     DAPGWD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.331"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..498
                     /locus_tag="RHX88_004526"
     CDS             <1..498
                     /locus_tag="RHX88_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2233112.1"
                     /translation="ISIATVIPWFIGAIGMVLGGVCSDFIYRITGNALLSRRLILGSC
                     LAGAAVCVAVSGTVTSIGSAITLMSVSLFLLYLTGPIYWAVIQDVVHKDKVGSVGGAM
                     HGLANISGIIGPLVTGFIVQFSGKYDYAFYLAGAIAIISSLLVFIFVKNKGLKVNESQ
                     SCVHQ"
     gene            470..>1428
                     /locus_tag="RHX88_004527"
     CDS             470..>1428
                     /locus_tag="RHX88_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDV2233113.1"
                     /translation="MKVKAVYINKPGEIATRLVDYPVRKDNEVLIKVDAAGICGSDIG
                     AFRGTNPLVTYPRIIGHEVTGIVLETGEGVPQNIKQGDRVIVDPYVYCGHCYPCSVGR
                     TNCCENLKVIGVHIDGAMQEVVSHPAHLIHKIPDNVPSEMAPLAEPLTIALHALHRAQ
                     LKAGEHVVIIGAGAIGLMAALSSLNYKATPILVDIVDERLQYAKSLGVPYVVNSAKAN
                     VIEAVKDITQGTMAQVVVEASGANSAIRSTLDLASFAGRIAYTGWPKQETSLPTNLIT
                     FKELDLRGSRTSAGEFEEALQMLSTLNINPADIVSKVVTIDEVP"
BASE COUNT          372 a          280 c          348 g          428 t
ORIGIN      
        1 attagtattg caactgttat cccgtggttt attggggcaa ttggcatggt gttaggtggc
       61 gtctgctctg actttattta tcgtattacg ggaaatgcct tactttctcg ccgtttaatt
      121 ctcggcagtt gccttgcggg ggccgcggtt tgtgtcgctg tttccggaac tgttacctct
      181 attggtagtg cgattacgct gatgtctgtt tccttattcc tgctttatct aactggacct
      241 atttattggg ctgttattca ggatgtagtg cataaagata aagtaggtag cgtaggcggt
      301 gcgatgcacg gtctggcaaa tatttccggc attattggtc cattggtaac cggttttatt
      361 gtccagttca gtggtaaata tgattacgca ttctatcttg caggtgccat agcaatcatt
      421 tcaagtctgc tggtattcat ttttgttaaa aataaaggat tgaaagtcaa tgaaagtcaa
      481 agctgtgtac atcaataaac ctggtgaaat tgctacccgt ctggtcgatt atccagtaag
      541 aaaagataat gaagttttaa ttaaagtgga tgcagcggga atttgtggtt ctgacatcgg
      601 tgcatttcgt ggtactaacc cactggtaac ttatccgaga attattggac atgaagtcac
      661 cgggattgtt ctggaaaccg gtgagggggt accacaaaat atcaaacagg gcgaccgggt
      721 tattgtagat ccctatgttt attgcggtca ctgttatcca tgttctgtag gtagaactaa
      781 ttgctgcgag aacttaaaag tcatcggcgt acatattgat ggcgctatgc aagaagtggt
      841 ttcgcatccg gctcacttaa ttcacaaaat tcctgacaat gtaccgtcag agatggcgcc
      901 actggctgaa cccttaacta ttgccctaca cgcattacat cgagcgcaat tgaaagcagg
      961 tgaacatgtc gtcattattg gtgcaggcgc tattggactt atggcggccc tgagttcgtt
     1021 gaactataaa gcaactccca ttctggttga tattgtcgat gaacggttgc aatacgcgaa
     1081 atcgctcggc gtaccttatg tagtaaactc cgcaaaagct aatgttattg aggcagtgaa
     1141 agatattaca caagggacga tggctcaggt tgtggtggaa gcctctggtg caaatagcgc
     1201 aatcagaagt accctggatc tggcctcatt tgcagggcgt atagcctata ctggttggcc
     1261 aaagcaggaa acatcgttac caactaatct aattaccttt aaagaactag atttacgtgg
     1321 ttcccgtacc agtgccggtg aatttgaaga agccctgcaa atgttatcaa cgctgaatat
     1381 taatccggct gatatcgtga gcaaagtcgt gacaatagat gaagttcc
//
LOCUS       DAPGWD010000328         1417 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPGWD010000328 DAPGWD010000000
VERSION     DAPGWD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.332"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1417
                     /gene="rhsB"
                     /locus_tag="RHX88_004528"
     CDS             <1..>1417
                     /gene="rhsB"
                     /locus_tag="RHX88_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDV2233114.1"
                     /translation="TQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATD
                     DATGSRKTMTWSRYGQLLSFTDCSGYQTRYDHDRFGQMTAVHREEGLSQYHAYDSRGQ
                     LTAVKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVKGETAERWRYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGER
                     QTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLSGMKLGDTPL
                     VEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLQYDRDYTWNDNGELIRISS
                     PRQTRSYSYSTTGRLTSVHTTA"
BASE COUNT          339 a          451 c          422 g          205 t
ORIGIN      
        1 cacgcagagt cagtttgacg ccgtgggcag gctcaggaca cagacggatg ccgcaggccg
       61 gacaacagaa tacagcccgg atgtggtgac gggcctcatc acgcgcatca ccacgccgga
      121 tggcagggca tcggcgtttt actataacca ccacagccag ttaacgtcag ccaccgggcc
      181 tgacgggctg gaattgcgcc gggaatatga tgaatccggc cgtctgattc aggaaaccgc
      241 ccctgacggc gatatcaccc gctaccgtta tgataatcca cacagtgacc tcccctgcgc
      301 aacggacgat gccaccggca gccggaaaac catgacgtgg agccgttacg gccagttgct
      361 gagcttcacc gactgttccg gctaccagac ccgttatgac catgaccgct tcgggcagat
      421 gacggcggtg caccgcgagg aagggctgag ccagtaccac gcatacgaca gccgtggaca
      481 gttaactgcg gtgaaagaca cgcagggcca tgaaacgcgg tatgaataca acatcgccgg
      541 tgacctgacc gccgtcattg ccccggacgg cagcagaaac gggacacagt acgatgcgtg
      601 gggaaaggcc gtccgtacca cgcagggcgg gctgacccgt agtatggaat acgatgctgc
      661 cggacgggtc atccgcctga ccagtgaaaa cggcagccac accaccttcc gttacgatgt
      721 actcgaccgg ctgatacagg aaaccggctt tgacggccgc acacagcgtt atcaccacga
      781 cctgaccggc aaacttatcc gcagcgagga tgaggggctg gtcacccact ggcactatga
      841 cgaagcagac cgcctcacgc accgcaccgt gaagggtgaa accgcagagc ggtggcggta
      901 cgacgaacgc ggctggctga cagacatcag ccatatcagc gaagggcacc gggtgacggt
      961 gcattacggg tatgatgaga aaggccgcct gaccggtgag cgtcagacgg tgcatcaccc
     1021 gcagacggaa gcactgctct ggcagcatga gaccagacat gcgtacaacg cgcaggggct
     1081 ggcgaaccgc tgtataccgg acagcctgcc cgccgtggaa tggctgacct acggcagcgg
     1141 ctggctgtca ggcatgaaac tcggcgacac accgctggtg gagtacaccc gcgaccgcct
     1201 gcaccgggaa acgctgcgca gcttcggtcg ttatgaactc accaccgctt acacccctgc
     1261 cgggcagtta cagagccagc acctgaacag cctgcagtat gaccgcgatt acacctggaa
     1321 cgacaacggc gaactcatcc gcatcagcag cccgcgccag acccggagtt acagctacag
     1381 caccaccggc aggctgacca gcgttcacac caccgca
//
LOCUS       DAPGWD010000329         1407 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPGWD010000329 DAPGWD010000000
VERSION     DAPGWD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.333"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1407
                     /gene="rhsB"
                     /locus_tag="RHX88_004529"
     CDS             <1..>1407
                     /gene="rhsB"
                     /locus_tag="RHX88_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDV2233115.1"
                     /translation="TQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHNQLTSATGPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLTFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQ
                     LTAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVNGETAEQWQYDERGWLTDISHISKGHRVTVHYGYDEKGRLTGER
                     QTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPL
                     VEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISS
                     PRQTRSYSYSTTGRLTGV"
BASE COUNT          339 a          437 c          416 g          215 t
ORIGIN      
        1 cacgcagagt cagtttgacg ccgtgggcag gctcagggca cagacggatg ccgcaggccg
       61 gacaacagag tacagcccgg atgtggtgac gggcctcatc acgcgcataa ccacgccgga
      121 tggcagggca tcggcgtttt actataacca ccacaaccag ttaacgtcag ccaccgggcc
      181 tgacgggctg gaattgcgcc gggaatatga tgaatccggc cgtctgattc aggaaaccgc
      241 ccctgacggc gatatcaccc gctaccgtta tgataatcca cacagtgact taccctgcgc
      301 aacggaagat gccaccggca gccggaaaac catgacgtgg agccgttacg gtcagttgct
      361 gaccttcact gactgttccg gttatgtaac ccgctatgac catgaccgtt ttggtcaggt
      421 gacggcggtg caccgcgagg aaggcctgag ccagtaccac gcatacgaca gccgtggaca
      481 gttaactgcg gtgaaagaca cgcagggcca tgaaacgcgg tatgaataca acgccgccgg
      541 tgacctgacc gccgtcattg ccccggacgg cagcagaaac gggacacagt acgatgcgtg
      601 gggaaaagcc atctgtacca cgcagggcgg tctgacgcgc agtatggaat acgatgctgc
      661 cggacgggtc atccgcctga ccagtgaaaa cggcagccac accaccttcc gttacgatgt
      721 actcgaccgg ctgatacagg aaaccggctt tgacggccgc acacagcgtt atcaccacga
      781 cctgaccggc aaacttatcc gcagcgagga tgaggggctg gtcacccact ggcactatga
      841 cgaagcagac cgcctcacgc accgcaccgt gaatggcgaa accgcagagc agtggcagta
      901 tgacgaacgc ggctggctga cagacatcag ccatatcagc aaagggcacc gggtgacggt
      961 gcattacggg tatgatgaga aaggccggct gaccggtgag cgtcagacgg tgcatcaccc
     1021 gcagacggaa gcactgctct ggcagcatga gaccagacat gcgtacaacg cgcaggggct
     1081 ggcgaaccgc tgtataccgg acagcctgcc cgccgtggaa tggctgacct acggcagcgg
     1141 ttacctggca ggcatgaaac tcggcgacac accgctggtg gagtacaccc gcgaccgcct
     1201 gcaccgggaa acgctgcgca gcttcggccg ttatgaactc accaccgctt atacccctgc
     1261 cgggcagtta cagagccagc acctgaacag cctgctgtct gaccgcgatt acacctggaa
     1321 cgacaacggc gaactcatcc gcatcagcag cccgcgccag acccggagtt acagctacag
     1381 caccaccggc aggctgaccg gcgttca
//
LOCUS       DAPGWD010000330         1404 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPGWD010000330 DAPGWD010000000
VERSION     DAPGWD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.334"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(56..1084)
                     /locus_tag="RHX88_004530"
     CDS             complement(56..1084)
                     /locus_tag="RHX88_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDV2233116.1"
                     /translation="MILGNDYVDLAPLFSAHSTRNYLLSTLDFTDPVGVSSHKVAVSQ
                     LVESNESLFNKETSRFSSEHNVTKREQGKEWLIEIPYFLREDVIRPSDVQGKRKPGTD
                     FQETLTDIYADYVAKHHVAYQRTKESVLAASLFSGKTYTPKTDDVLIEWGKLFNVSAM
                     KATVNASSTDTTKIFKEFDQIATDIIEKAQSQAAAVERIVVFCKPEAFSAIRFSAGMA
                     NAFQYVSPLEEGNVVYQRRDLLPGVTAFTIPGTNIDVVKLVDPLHLAHMTADAVAVPK
                     FAKGSNVYQNIYGAASSTFELINAAPAEVYSYSYESSRGDAVNVVTENSQMVVNHGVG
                     FSVQITVK"
     gene            complement(1103..1330)
                     /locus_tag="RHX88_004531"
     CDS             complement(1103..1330)
                     /locus_tag="RHX88_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233117.1"
                     /translation="MITSTGAKYESGDDIYVVMSEFLTAGANKPVDVLRSNAGLVCIK
                     ADALNAVSEAAKTALIKKGFQLEGFHSVFTS"
BASE COUNT          421 a          311 c          285 g          387 t
ORIGIN      
        1 ataaaaaaaa ggcgaagcgt gaatacacgc cacgcctcta ttacacgtta aaatattatt
       61 taacagtgat ttgaacggag aagcctacac cgtggtttac aaccatctgg ctattctctg
      121 taacaacgtt aacagcatca ccgcgagaag attcatagct atagctatac acctcagcag
      181 gagcggcatt aatcagttca aaggtgctgg aagcagcccc gtagatgttc tgatatacat
      241 tagagccttt agcaaacttc ggaacagcta cagcgtctgc ggtcatatgt gcaaggtgta
      301 gcggatctac cagtttaaca acatcaatgt tagttcctgg aatagtgaac gctgtcactc
      361 ctggtagcag gtcacgacgc tgatacacaa cgttcccctc ttccagtggg cttacatact
      421 ggaatgcgtt tgccataccc gcagagaatc gaattgcaga gaaggcttcc ggtttacaga
      481 aaacaacaat tctttctaca gcagccgcct ggctttgtgc tttttcgata atgtcagtag
      541 caatctgatc aaattcttta aaaatcttcg tggtgtcagt gctggaagcg ttcacagtgg
      601 ctttcatagc tgatacgtta aacagcttgc cccactcaat taacacatca tcagttttag
      661 gcgtgtaagt tttaccgcta aacagagatg cagccaatac gctctctttt gtacgctgat
      721 acgctacgtg gtgtttagca acatagtcag catagatatc agtgagtgtt tcctgaaaat
      781 cagtgccagg cttacgcttg ccttgaacat cagaaggtcg aattacatct tcacgaagaa
      841 aatacgggat ttcaatcagc cattctttac cttgttctcg cttagtaaca ttgtgttcag
      901 aagagaaacg agaggtttct ttgttgaaca gagattcgtt gctttcgaca agctgggaaa
      961 cagctacttt atgagaacta acgcccaccg gatcagtgaa gtcgagagta gaaagcagat
     1021 agtttctagt gctatgagca gagaacagag gagctaaatc aacgtaatca ttacctagaa
     1081 tcatttatta ttattccttt tattaagaag tgaaaacaga atggaaccct tcaagctgga
     1141 agcctttttt aattaatgct gtttttgctg cttcacttac agcattcagc gcatcagctt
     1201 taatgcatac aagcccagca ttgctacgta aaacatctac aggcttgttt gctccagccg
     1261 ttaaaaattc actcataaca acataaatat catctcctga ttcatattta gcgcccgtgc
     1321 tggtgatcaa gtctccgcat tcaacatcag caggaagaga agcaaaaacc ttgctttcta
     1381 cagaggcaaa cggatcagta caat
//
LOCUS       DAPGWD010000331         1343 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPGWD010000331 DAPGWD010000000
VERSION     DAPGWD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.335"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(123..308)
                     /gene="rzoD"
                     /locus_tag="RHX88_004532"
     CDS             complement(123..308)
                     /gene="rzoD"
                     /locus_tag="RHX88_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HDV2233118.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCASKQSVSQCVKPPPPPAWIMQSPP
                     DWQTPLNGIISPSGNDW"
     gene            complement(525..1022)
                     /gene="rrrD"
                     /locus_tag="RHX88_004533"
     CDS             complement(525..1022)
                     /gene="rrrD"
                     /locus_tag="RHX88_004533"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDV2233119.1"
                     /translation="MPPSLRKVVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVCTVCHGHTGKDIMLGKTYTKAECKTLLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(1022..1237)
                     /gene="essD"
                     /locus_tag="RHX88_004534"
     CDS             complement(1022..1237)
                     /gene="essD"
                     /locus_tag="RHX88_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV2233120.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQLLDRVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          333 a          332 c          301 g          377 t
ORIGIN      
        1 agatcttact tcactgttcc ttatgaaaca atcatcaaaa tgaatcatat tgtagttaag
       61 attttacttt aaacactgtt cggttatgta ttgctgagca ccttcaagtt gggcctgcat
      121 cattaccagt cgttcccgga gggtgaaata atcccgttca gcggtgtctg ccagtctggg
      181 ggagactgca ttatccacgc tggaggtggc ggtggcttca cgcactgact gacagactgc
      241 tttgatgcgc aaccgacgac gaccagcggc aacatcatca cgcagagcat cattttcagc
      301 tttcgcatca gctaactcct tcgtgtattt tgcatcgagc gcagcaacat cacgctgacg
      361 catctgcatg tcagtaattg ccgcgttcgc cagcttcagt tctctggcat ttttgtcgcg
      421 ctgttctttg taggcgatgg cgttatcacg gtaatgatta acaccccatg acaggcagac
      481 gacgatgcag ataaccagag cggagataat cgcggttact ctgttcattg ctgaccccac
      541 aaacagattt cacgctcaat ctcacgacga gtcatgagac ctttccattg cttaccgcca
      601 gcatatgtcc agcgacgtag ctgatcacat gcgcctttga tatcgccctg gtttattttg
      661 cgaagaagcg tcgatgttct gaaattgcca gcacccacgt tgtaaacgaa tgagtaaaga
      721 gcgccgcgcg ttgtttccgg tatatcgact ttgatgtacg ggttaatttg tctggcgacc
      781 gtggcaaggt ctttattcaa gagtgttttg cattctgctt tggtatacgt tttaccgagc
      841 atgatgtctt ttcctgtatg cccgtgacat acagtacata caccaacaat atctttgtat
      901 ggtatgtagc tgacaccttc cagaccatcg ttaccacttg ggccagtgat taacactgat
      961 gctatagcaa ttgctccgcc accaatagca gcagcaacga cttttcgtaa tgatggaggc
     1021 attattcacc tctcgcagcc ttgcgcttat cttctttaat cttgaaataa aggtttgtca
     1081 ggtacgtcag taggccaaat accagactac ccagcacacc gattgcagcc cactgtgacg
     1141 gagttactct atcgagcaac tgtaaaaacc agtagccagc actgcctgcg gaggtgccgt
     1201 aggcaatgcc tgttgaaatt ttgtccatgg atttcatagc ctcacctccg cacggaacgg
     1261 atggcatagt tattatgtgt aggctttcag acacatcaat cagagcctta attgatatat
     1321 atgctggaga cgatgcaata taa
//
LOCUS       DAPGWD010000332         1339 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPGWD010000332 DAPGWD010000000
VERSION     DAPGWD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.336"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            143..412
                     /locus_tag="RHX88_004535"
                     /pseudo
     CDS             143..412
                     /locus_tag="RHX88_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          402 a          300 c          330 g          307 t
ORIGIN      
        1 actcattccc tgcttgtgca ggattttaat ttccataact gtctcaaaag tgaccataag
       61 ctctcctgaa tcaggagagc agattacccc ctggatctga tttcaggcgt tgggtgtgga
      121 tcactattgc accgttcgtg acaacaaaag gggtaagctg aaatcgttta tggattacat
      181 cgacatatgc ctggcagaga atgtccggaa tgacgaggtt atctttgcgg agattaaggc
      241 aatggtatac gggccgacgt tccatgccgc gttactacat tcagcccgga cgtaaaatgc
      301 gcccgtcaaa aagaacggtt cgcttcgaaa ctcagccagg ataccacctc tccgccacac
      361 ccgggaagaa gttgatatgg tggttgccgg acaacagcgc tcctggattg actgctgcat
      421 cactcaacca cgctgaatat caaaggagag cgttaccggt taaaagagaa acgtaaagct
      481 gtggtgatga cccaaaacgc aacgcctgtc agtgaggatg aaatggtgaa aagcggacag
      541 catcagtaac ggaagtatct tagcgggtac gaaaatggca ggatcgtgac attgacagaa
      601 tatctcctga tcatcctgaa tgactgcagt tgctgtagaa aagtaagctg tctccaccca
      661 cggcaccact taacaggcat tccgtaactt cccccagact tactatgacg cattatcggt
      721 gcactgaaaa cgggagagtg cgcttaaaac cgcagaccgc atgcgggtgt tactgcatga
      781 cagtctggcc gtattgtgag atgtcggcag gaaccgatgt ccgcgcaggg gggaacttcc
      841 tgcagtacct gacgcttcaa atggtattaa ccgtagggtg ttgttggttc cggatcgcgg
      901 aaatgggagc acaggtctgc tacggggtgt acctctgaca gtatatccgg ctcgctcatc
      961 tgacagcctc tctcccggaa aagaccaaaa atgagatcgt ctcctcaaca gcagagaaga
     1021 ggtcttgaca gacaggctac attgcatacg atttatgaag aaaaagacag caaatatgga
     1081 ggttatagcg gagaataccc tacgaaattc ctatgaaaaa cgattgaaaa aaatatcaaa
     1141 ttcgattcgt ttttatatgc tttttgggag cgtggcgatg acgaactgtt gcatgtgcag
     1201 aggagaatgc acccggaagc accagaatca tccagaaagg cagaacgatt acgcatcaaa
     1261 ccaggagaca gcaatatcaa ggtaatatac tgatttaaaa tgaatttaca gcaatacata
     1321 aaaacatttt ttacttaaa
//
LOCUS       DAPGWD010000333         1325 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPGWD010000333 DAPGWD010000000
VERSION     DAPGWD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.337"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            183..785
                     /locus_tag="RHX88_004536"
     CDS             183..785
                     /locus_tag="RHX88_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDV2233121.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(824..1249)
                     /gene="nudI"
                     /locus_tag="RHX88_004537"
     CDS             complement(824..1249)
                     /gene="nudI"
                     /locus_tag="RHX88_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDV2233122.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          367 a          287 c          276 g          395 t
ORIGIN      
        1 tagacgtaaa tattgaaatt tttatatttt ttcttattta ggctttgcat ttggcaaaat
       61 tttgaggcat tttgccgaca tcgtaggttt tttaatatta caccaactgc taattatcgc
      121 cagaaatgtc gctcaatttt acggtaattg tctggttgcg cttgtctata ggtggagttt
      181 acgtgttagc tttttgccgc tcatcattga agtcaaaaaa atatttcatc attttactgg
      241 cgctcgctgc aattgccgga ctgggtactc atgccgcctg gagtagcaat ggtttgccac
      301 gtatcgacaa taaaacattg gccagactgg cacagcagca cccggttgtc gttttgtttc
      361 gccatgctga acgttgcgat cgttcaacca atcaatgctt gtcagataaa acaggtatta
      421 cggttaaagg tacccaggat gcccgtgaac tgggcaacgc ttttagcgct gacatccctg
      481 atttcgatct ttattccagt aataccgtcc ggaccattca gtcggcgacc tggttttccg
      541 cgggtaaaaa gctgacggta gataaacgac ttcttcagtg cggtaatgag atttatagtg
      601 caattaagga cttacaaagc aaagcgcctg ataaaaatat cgttattttc acccataatc
      661 attgcctgac atatattgct aaaaataagc gtgatgcgac atttaaacct gattatctgg
      721 atggtttagt catgcatgtg gaaaaaggca aagtttatct ggatggggaa ttcgttaacc
      781 actaaatttt caatctgaca gccagtgatg gctgtcatca ttgttaaaga agacctttca
      841 aacgtaacgt ttttcgggtg gcaacattta aatcataatg caccagatct tcaggtttta
      901 cccacgcgta gtcctgaaac tcttcgttta ttttcacttc tcggttggca gaaacgcagt
      961 caaaaatcag gtaaatcata taaatttctt ccttgcgacc atctgcatac gtcttggtgc
     1021 gaatatcatc gctgaaggtc cacggcgtga tttctgtcaa aagcagctgt tctcccagtt
     1081 cttcgcgaat ttcgcggcgt agtgcctctt caattcgttc gccagactcc acgccgccac
     1141 ccgaaagcgc ccattgaccg gggaaaacgc cgcgatcgtc ggccatttta cacagcaaat
     1201 aagcaccatc attttgaatc aaagggcata caatagtccg ttgtcgcata ggggtattcc
     1261 ttataaataa aaagaaacca tgagaatacg gtaactcaga aatatgtgaa agtgacagta
     1321 atctc
//
LOCUS       DAPGWD010000334         1320 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPGWD010000334 DAPGWD010000000
VERSION     DAPGWD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.338"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            33..437
                     /gene="stpA"
                     /locus_tag="RHX88_004538"
     CDS             33..437
                     /gene="stpA"
                     /locus_tag="RHX88_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDV2233123.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(484..1008)
                     /gene="ygaP"
                     /locus_tag="RHX88_004539"
     CDS             complement(484..1008)
                     /gene="ygaP"
                     /locus_tag="RHX88_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDV2233124.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPFPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1018..1317)
                     /locus_tag="RHX88_004540"
     CDS             complement(1018..1317)
                     /locus_tag="RHX88_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2233125.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          346 a          319 c          319 g          336 t
ORIGIN      
        1 ttttgttttg gcgttaaaag gttttcttta ttatgtccgt aatgttacaa agtttaaata
       61 acattcgcac cctccgtgcg atggctcgcg aattctccat tgacgttctt gaagaaatgc
      121 tcgaaaaatt cagggttgtc actaaagaaa gacgtgaaga agaagaacag cagcagcgtg
      181 aactggctga gcgtcaggaa aaaattagca cctggctgga gctgatgaag gctgacggaa
      241 ttaacccgga agagttattg ggtaatagct ctgctgctgc accacgcgct ggtaaaaaac
      301 gccagccgcg tccggcgaaa tataaattca ccgatgttaa cggtgaaact aaaacctgga
      361 ccggtcaggg ccgtacaccg aagccaattg ctcaggcgct ggcagaaggt aaatctctcg
      421 acgatttcct gatctaatta ctgaggccgg atgtcgctgc tagggcgcgt ccggcgtcgc
      481 ttctcaagct cgttggttcc acggcatctt atctaacaac cttgccattc cacaaaaacc
      541 gctgattcct gcaaacagta acccggcacc aacaaagcca cttaataaga agaaaccgct
      601 atttacggta taacccagta caacgccgat taatattaaa ccacccgcag cgatctgcac
      661 ctggcgcatt aacggaaagg gttgagattt atttaccgct actggcaatc cagctctttt
      721 ccagccatca atcccatctt cgagtaaaaa tatttctgcg ggggcggcaa tcgctgctaa
      781 tttatcggca ttattactgg tgcgtttacc tgcctggcag tggaatataa tttgctcgtg
      841 acgtaattta gccggaagac ctgactgttc cagcacggat aatggagcca gatctgcttc
      901 aggaatatgt tcacgaagat attcatcagc atcacgaata tcgattaact ttgcgccgcg
      961 tgcgattaat tcttgtgcat catgcggcga aatggttgtc aaagccatag tgactcctta
     1021 cggacaatag acatttttca gggtggcgat aatggcattt accgcctcat ttttaatgga
     1081 atatagaatg cgttgggcat cccgttggct gtcgataagc ccttcgtccc gcatacgagc
     1141 gagatgctgt gatgtcgcag aggcactcag tccggtaatg cgcgtcagct ctcccgcgct
     1201 ggtgccgggg gaaccgctaa gcatgcacag aatcagcaat cgtttagggt ggctcattgc
     1261 tttcaataag gccgctgcct gttcggcact ggcctgtaat tgcgcgagtt cagtcatatt
//
LOCUS       DAPGWD010000335         1315 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPGWD010000335 DAPGWD010000000
VERSION     DAPGWD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.339"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>1315)
                     /locus_tag="RHX88_004541"
     CDS             complement(<1..>1315)
                     /locus_tag="RHX88_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2233126.1"
                     /translation="GSAVGTTINGGLQTVFGGGSVSGTLINNGEQRVSSGGSAIDTTI
                     NNGGTMSIAAGGSGTGIIQNNGGAIRANTSAILSGTNVNGSFSITNGSARNMLLENGG
                     LLMVLQGHQAIDTQVSKGGELQVESGSELLGTTLIKDDGKLSGNQITNNGVLHFLDNS
                     SANYSGTLDGTGSLNLDGGKLTLSGNFSQDSGIFIQNHGTMIMDNLRAMANVTIEPDA
                     TLRLENGSLLAGRITGDGKKGGNLIVADSVWKLSGDSTINSLDMSNGTVDFHSIQSPQ
                     FKTKLEGVTLNIGQLSGNGTFLMNTDIASYTGDKLNITGNANGNFVLNIKNTGQEPVS
                     PGNPLPVVYTGGGDAVFTVNGGKVDAGVWEYYLSKDNTNWYLKSDTTQPGNPEPGIPN
                     PPVRHTTKSTDAVLNMASAPTYIFNSELQGLRFRNSEKSENSRTSG"
BASE COUNT          335 a          332 c          247 g          401 t
ORIGIN      
        1 ccagaagtac gtgagttttc tgatttttca ctgttacgga atcgcagccc ttgtaattca
       61 ctgttaaaga tatatgtcgg agcgcttgcc atattcagta cagcatcagt cgactttgtg
      121 gtgtgccgta ctggaggatt gggtataccc ggttccggat tacctggttg cgtagtatca
      181 gattttagat accagttagt attatcttta ctcagataat attcccaaac tcctgcatcc
      241 acttttcctc cgtttactgt aaatacagca tctcctccac cggtataaac cactggtaat
      301 gggttacccg gggatacagg ctcctgaccg gtatttttga tattcaaaac aaaattaccg
      361 tttgcattcc ctgtgatgtt taatttatca ccagtatatg atgcaatatc agtattcatc
      421 aagaaggtgc catttcctga taactgtccg atatttaacg ttactccctc aagttttgtt
      481 ttaaactgag gggactgtat tgaatgaaaa tccacggttc cattactcat gtctaatgag
      541 ttaatagtag agtctccgga taatttccag acagagtcag ccactatcag atttccacct
      601 ttcttcccat ccccagtaat gcgcccagcc aggaggctac cattctctaa gcgcagagtt
      661 gcatcaggct cgattgtcac atttgccata gcccgtagat tatccattat cattgttcca
      721 tgattttgga taaaaatacc gctatcctga gaaaaattac cactgagtgt taattttccc
      781 ccatccagat tcagactacc ggtaccatcc agtgttccgc tatagtttgc tgaactattg
      841 tcgagaaaat gcagaacccc attattggtt atctggttgc cagacagttt gccatcgtct
      901 ttaatcaagg ttgtacccaa tagctcagaa ccgctctcaa cctgtaattc accaccttta
      961 gagacctggg tatctatagc ctgatggcct tgcagaacca tcaggagtcc accattttct
     1021 aacaacatat ttctggcgct accattagta atgctgaagc taccatttac gttcgttcca
     1081 ctcaggatgg ccgatgtgtt cgctctgata gcgccaccat tgttttgaat tattcccgtt
     1141 ccactaccac ctgcggcaat actcattgtc cctccattgt taatggttgt gtcaatagca
     1201 ctgccaccac tggagactcg ctgctcaccg ttattaatga gagtcccgga gacacttccg
     1261 ccaccgaaaa cagtctgcag tcccccatta atggtggtgc ctacagcact accac
//
LOCUS       DAPGWD010000336         1306 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPGWD010000336 DAPGWD010000000
VERSION     DAPGWD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.340"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(10..1305)
                     /locus_tag="RHX88_004542"
     CDS             complement(10..1305)
                     /locus_tag="RHX88_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV2233127.1"
                     /translation="MVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYF
                     IYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSL
                     CILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNLHIAE
                     LTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLL
                     FMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFF
                     NELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLL
                     ARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQE
                     NALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
BASE COUNT          430 a          293 c          215 g          368 t
ORIGIN      
        1 aatgccgctt tatggtgact cacaaaaatt caccacatcc ctcacaacca ccttattacg
       61 cccggtctct ttcgcctcgt aaagtgcgtt atcagccagt cgatagatct catttgggtt
      121 taacgcattt tcctgagtca caacagcgcc aatactaatc gtcacttttt gtggtatagc
      181 gtattcagga ttatcgccag ttaaacgctc aacattttcc cgaatccttt ccgctaaagc
      241 tttcgcacgt tcagtatcga tgtccgtcag caagacgcca aactcttcgc cgccgacgcg
      301 cgctaaaagg tcatctggtc gtatgctttt cccaatgata ttcaccactg tttttatcac
      361 ctgatcgcca accggatgcc cccaggtgtc gttgactttt ttgaagtggt cgatatccat
      421 aatcatgacg caataaggcg ttttttgggc tgatgctgat tgaactgtca gttcattaaa
      481 aaaataattt ctgttaaata tattggttaa gggatcgcga tgtgcaatgt tctttgttac
      541 tcgtagcgca ctgaatatat ggcacataaa tattaccata acggttaact tactgactac
      601 ttcaatagtg cgactaatat accaagtgga cacgctatac ctgctcataa ataacagaga
      661 gatattgtac agtacggcac aaaaagcaat caccgtcact ccattccaga tatcatagcg
      721 taaaccatta aagtatagat taaccgaaag caaggttaac cacatgaaaa tcattatggt
      781 aacatatgag gctttccaaa ccacctgacc attttcattg gttaactcgg caatgtgtaa
      841 attgtagctc tcataatgac tagaaagtat gtgagcaact atcggccccc caaaaacaaa
      901 aaacaaactt atgcacaaag tcattttttt ggaaaataaa ttgcgctgtt ttgtgttttc
      961 actaacttta ttcaccaatg ccagaaatat taatatgcac aaactcatct gacgaaacaa
     1021 ataatagatt gcaatgtcat tttggacaac actgcttgtc aaacgcccct caataatttg
     1081 ctgaatgata atgacggttt taacaaagta aatcaaacaa ctcagaaaag ccaaactaag
     1141 tatacagttt gaaaattgcc tacggtcata aatgaaatac aactgcataa aaataaatgc
     1201 aacagtatcg ataaaaaata aagcaatcat taccatcaga taggttgatc gtggaacaac
     1261 ttcctcaacg cctggctgat taaaccaact attcaccaaa accaaa
//
LOCUS       DAPGWD010000337         1298 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPGWD010000337 DAPGWD010000000
VERSION     DAPGWD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.341"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1238)
                     /locus_tag="RHX88_004543"
     CDS             complement(<1..1238)
                     /locus_tag="RHX88_004543"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV2233128.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGG"
BASE COUNT          307 a          352 c          345 g          294 t
ORIGIN      
        1 ccgccgagag tgaaggccgg aacctgccag ccgcgcagac gcagacgttc agagaggtcg
       61 tacagggtgt atcccggatc ttcaccctct ttcagtttga agcaaaccgc cgggatgcct
      121 tcgtccggac gacccgtaca gatgaactca tacggcccca gtttggcgat ttcatccgcc
      181 agataagcgg caacctggta agaggcgttc tgtactttgg tatagccttc acgaccgagg
      241 cgcaggaatt cataatactg cgcaatcacc tgacccgccg ggcgggagaa gttgatggcg
      301 aaagtaccaa tctgaccacc gaggtagtca acgttgaaca ccagttcctg cggcagcgct
      361 tcttcatcac gccagataac ccagccgcag cccagcggag ccagaccgaa tttatggcct
      421 gaagcactga tcgatttcac acgcggcagg cggaagtccc agacgatatc cggggcgacg
      481 aacggtgcca ggaagccacc gctggcagcg tcgatgtgca tgtcgatgtc gataccggtg
      541 tcggcctgga atttatccag tgcatcgtgc agcggttgcg ggaactcata gttaccggtg
      601 taggtcacgc cgaaagtcgg caccacgccg atggtgtttt cgtcgcaggc ttcaatcatg
      661 cgtttcgggt ccataaacaa ctgaccgggg cgcataggga tctcgcgcag ctccacatcc
      721 cagtagcgag cgaatttatg ccagcagatt tgcaccggac cgcacaccag gtttggttta
      781 tccgttggtt tgcctgcagc ttccatacgc ttgcgccaac gccatttcat cgccatcccg
      841 ccaagcatac aagcctcgga agaaccaatg gtgttggtgc caacggcctg accatttttc
      901 ggcgcaggcg catgccacag atcggcaacc atgtttacgc aacgcaggtc gatggctgcg
      961 gattgcggat attcttcttt gtcgatccag tttttattga tcgacaaatc catcaattta
     1021 tggacgtttt cgtcgtccca ggtctggcag aaagtggcca ggttctgacg agcgttgcca
     1081 tcaagatata attcatcatt gataatctgg aatgcgacat catcgcgcat ttcgtgcagc
     1141 ggaaatcgtt ttgactccgc gatagtagaa atggcctttg cgccaaaacg tgaatcgagt
     1201 agttctgagc ggaaatccgt taacagcttc tggtccattt cgaactcctt aaatttattt
     1261 gaaggcaata aaaaagtagg atttatccgc aatggaag
//
LOCUS       DAPGWD010000338         1246 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPGWD010000338 DAPGWD010000000
VERSION     DAPGWD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.342"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..750)
                     /locus_tag="RHX88_004544"
     CDS             complement(<1..750)
                     /locus_tag="RHX88_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233129.1"
                     /translation="MIMSTPWLTPIVADSDHAEANAVSYEALTPTELDSDKAGCYISA
                     LNYAYEHPDIRNIAVTGPYGAGKSSVLKTWCKAHNGTLRVLTVSLADFDMQRHVDESN
                     GDSSSDEGTKNTGSVEKSIEYSILQQILYKNKKHELPCSRIDRISDVTAGQILRSASF
                     LTGTILLSGAALFFLAPDYVTTKLSLPGAFARYLLECPFGVRVSGAVASVMGSLCLLL
                     NQLHRIGIFDRKVSLDKVDLLKGAVTTRASSP"
     gene            994..1155
                     /locus_tag="RHX88_004545"
     CDS             994..1155
                     /locus_tag="RHX88_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233130.1"
                     /translation="MVRLDGSTCLQLWNKEGRVVRREGDPLEVAQWLQACHDAGIEVR
                     VQVNESVTP"
BASE COUNT          330 a          302 c          281 g          333 t
ORIGIN      
        1 aggtgatgat gcccgggttg taacagcgcc tttcagaagg tccactttat caagacttac
       61 tttcctgtca aatataccga tacgatgtaa ctggttcaaa agcaggcata acgatcccat
      121 cacagatgcc actgcaccgg acacacgcac cccaaacggg cattcaagaa ggtaacgggc
      181 gaatgctccc ggcaaagata gctttgttgt aacgtaatcc ggcgcaagga aaaataaagc
      241 agctccactc agtaaaatgg ttcctgtcag aaaagacgca gaccgcaata tttgtcccgc
      301 agtcacatct gatatacggt caatgcggga acagggaagc tcatgctttt tatttttgta
      361 gagtatttgt tgcagaatac tgtattcaat agatttttca acactaccag tatttttcgt
      421 cccttcgtca ctactgctgt ccccattact ttcatccaca tgtctctgca tatcaaaatc
      481 agcaagagaa acggttaaca cccgcagtgt cccattgtga gctttgcacc atgtttttaa
      541 tactgagctt ttccctgccc catacggccc ggtaacagca atattccgga tatccggatg
      601 ttcataagca taattaagcg cgctgatata acagcctgct ttatctgagt cgagttctgt
      661 cggagtcagt gcttcatagc tcactgcatt tgcctcagca tgatcactat cggcaacgat
      721 cggtgtcagc cacggtgtgg acattatcat tgatacaatt ccgttgttgt tttagttaca
      781 ttttactggt tttattccgc ttttatctca agcatggatc tctgaatttc ttaacttaac
      841 cagacgagaa gaaagcaggc aaaggcaacc gtacttacct gaagagacga ctgtgatagc
      901 ataaatcgat ttttaagacg ataattttcc tcttaccgta cgttttcgta cggttggccc
      961 tcaaacccct atcaggcctg ccgtggggat tttgtggtgc gtctcgacgg cagcacctgt
     1021 ctgcagttat ggaataaaga aggcagagtg gtacgccggg agggcgatcc gctggaagtg
     1081 gcgcagtggc tgcaggcctg tcatgatgcc ggaatagaag ttcgtgtaca ggttaatgaa
     1141 agcgtcactc cgtaaaaaca tgcccgcacg gtttacacat catcccatga tggcagccgg
     1201 ttaactttcc tgcgccacca gtggaaagag gccatgagca gtccga
//
LOCUS       DAPGWD010000339         1227 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPGWD010000339 DAPGWD010000000
VERSION     DAPGWD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.343"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..688
                     /locus_tag="RHX88_004546"
                     /pseudo
     CDS             1..688
                     /locus_tag="RHX88_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            718..1170
                     /locus_tag="RHX88_004547"
     CDS             718..1170
                     /locus_tag="RHX88_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233131.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWRDPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTYRSIKDAPQYPRGVRNVQNGTTRNVVKDQQLLKQLRKVDSGK"
BASE COUNT          401 a          194 c          338 g          294 t
ORIGIN      
        1 gagattacag ccattcctga acttcttaac atgatggata ttaaaggaaa aatcatcaca
       61 actgatgcga tgggctgcca gaaagatatt gcagaaaaga tacaaaaaca gggaggtgat
      121 tatttattcg cggtaaaagg aaaccagggg cggctaaata aagcctttga ggaaaaattt
      181 ccgctgaaag aattaaataa tccagagcat gacagttacg caatgagtga aaagagtcac
      241 ggcagagaag aaatccgtct tcatattgtt tgcgatgtcc ctgatgaact tattgatttc
      301 acgtttgaat ggaaagggct gaagaaatta tgcgtggcag tctcctttcg gtctataata
      361 gcagaacaaa agaaagagcc agaaattgac ggtcagatat tatatcagtt ctgctgattt
      421 aaccgcagag aagttcgcca cagcaatccg aaaccactgg cacgtggaga ataagctgca
      481 ctggcgtctg gacgtggtaa tgaatgaaga cgactgcaaa ataagaagag gaaatgcagc
      541 agaattattt tcagggatac ggcacattgc tattaatatt ttgacgaatg ataaggtatt
      601 caaggcaggg ttaagacgta agatgcgaaa agcagccatg gacaggaact atctggcgtc
      661 agtccttgcg gggagcgggc tttcgtaatc ttgccctggg taggaggccc tccagggatg
      721 ttagtcggtg cggcagttgg agcggtggtt attggtggtg gtatagtaat atacaacaat
      781 cgcgaggctg ttggggatgc gattgagaaa agtatggaga tgcggggtga agccgaatta
      841 ttaaatgcac aaagtcaaat ggaattctgg cgagatccct atggtgcttt ttccagcgtg
      901 tttggaaatg atgattcatc aaaagagaat ccaaatgtcg ggaaggattt gacggatgaa
      961 gagaagaact gtttgggcag tggcagtaca ggaggttcgg gagggtggga gccagatgag
     1021 gatggcgaaa taagaaatac ctacaggtct attaaggatg ctcctcagta tcccagaggg
     1081 gttaggaatg tccaaaatgg cactacaagg aatgttgtaa aggatcagca gttattgaaa
     1141 caattacgta aagtagattc aggaaaatag agtaaagttt ataaagatgg atttgatgcg
     1201 ccaggtcgaa aagtgtccat tcactat
//
LOCUS       DAPGWD010000340         1225 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPGWD010000340 DAPGWD010000000
VERSION     DAPGWD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.344"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            15..1112
                     /gene="ompC"
                     /locus_tag="RHX88_004548"
     CDS             15..1112
                     /gene="ompC"
                     /locus_tag="RHX88_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV2233132.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
BASE COUNT          346 a          272 c          271 g          336 t
ORIGIN      
        1 aaaaagggct cacgatgaaa aaattaacag tggcaatttc tgctgtagct gcatcagtac
       61 tgatggcgat gtctgctcag gcagctgaaa tttataataa agacagtaac aagctggatc
      121 tgtacgggaa agttaatgct aagcactact tctcctctaa tgatgcagat gatggtgata
      181 ctacttatgc ccgtcttggc ttcaaaggtg aaacccaaat caacgatcaa ctgactggtt
      241 tcggtcagtg ggaatatgaa ttcaaaggca accgcgctga atctcaaggt tcctccaaag
      301 acaaaacccg tcttgcattt gcaggcctga aattcggtga ctacggctca atcgattacg
      361 gccgtaacta cggtgtagca tacgacatcg gtgcgtggac tgacgttctg ccagaattcg
      421 gtggcgatac ctggacccaa acagatgtgt tcatgactgg tcgcaccact ggtgttgcaa
      481 cctatcgtaa caacgacttc tttggtctgg ttgatggtct gaactttgct gctcagtacc
      541 aaggcaaaaa cgatcgtagc gatttcgata actacaccga aggtaacggt gatggcttcg
      601 gtttctctgc tacctatgaa tacgaaggat tcggtatcgg tgcaacttat gcgaaatctg
      661 atcgtaccga cactcaagtt aatgcaggga aagttcttcc tgaagtattt gcttccggta
      721 aaaatgcaga agtttgggcc gcaggtctga aatatgacgc taacaacatt tacctggcca
      781 ctacctattc tgaaacccag aatatgactg tatttgctga tcacttcgtt gctaataaag
      841 ctcaaaactt cgaagctgtt gcacaatatc agttcgattt cggtctgcgt ccgtccgttg
      901 cttacctgca atctaaaggt aaggatcttg gagtatgggg cgatcaggac ttagtcaaat
      961 atgttgatgt aggtgcaacc tattacttca acaaaaatat gtctactttc gttgattaca
     1021 aaatcaacct gcttgacaaa aatgacttca ctaaagcact cggtgtaagc actgatgaca
     1081 tcgttgctgt aggtctggtt taccagttct aatctgatta cgaaaaagat atgttgcggg
     1141 aggcgttgcc tccccaacat ataagtggct ccctcaagcc acttccttta ggagcacaac
     1201 cttgcttcta actatataaa ccttc
//
LOCUS       DAPGWD010000341         1212 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPGWD010000341 DAPGWD010000000
VERSION     DAPGWD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.345"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            143..901
                     /locus_tag="RHX88_004549"
     CDS             143..901
                     /locus_tag="RHX88_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDV2233133.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(954..1061)
                     /locus_tag="RHX88_004550"
                     /pseudo
     CDS             complement(954..1061)
                     /locus_tag="RHX88_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          307 a          276 c          287 g          342 t
ORIGIN      
        1 aaaaaatgaa ataagaatac attgctaagt tattcgcgcc tttttataat tcgttacctt
       61 tccagggacg ctaatctttg atccttaaga aagtttccgg gaccgcgatg gtctcttttt
      121 ttgttgtacg gactcttgat aaatgaagct tttgaagaca gttcctgcca tcgttatgtt
      181 ggcggggggc atgtttgcgt cactgaatgc agctgccgat gattccgttt ttactgtcat
      241 ggacgatcct gccagcgcta aaaaaccttt cgaaggtaat ctgaacgcag gatatcttgc
      301 acaatcaggc aatacgaaaa gctcctcact tacggctgac accaccatga cctggtatgg
      361 acaaaccact gcctggtcgc tgtgggggaa cgccagtaat acttcttcta atgatgagcg
      421 ttcttcagaa aaatatgcgg cgggcgggcg tagccgtttt aatttaactg attatgacta
      481 tttatttggt caggcaagct ggctgacaga ccgttataac ggctatcgag agcgcgatgt
      541 gttgaccgcg ggttatggtc gtcagttttt gaatggcccg gtacacagtt tccgctttga
      601 attcggtcca ggtgtgcgtt acgacaaata taccgacaat gccagtgaaa ctcagccgct
      661 gggctatgcc tccggtgctt atgcatggca gttgactgac aacgctaaat ttacgcaggg
      721 tgtttccgtc tttggcgcgg aagacacaac gctgaactcc gaaagcgcat taaatgttgc
      781 tattaatgaa cattttggac tgaaagtggc ttacaacgtc acatggaact ctgaacctcc
      841 agagtctgca ccggaacata ccgatcgtcg gacaacctta tcgctgggct acagcatgta
      901 aaattatctg ccggaccgtg ttatgcggtc tggctgtctt cgtttgtcac tgctcagccc
      961 caaatcattg ctgcccaacg tagcaacaac atagcgccac agattgccag tagcaccacc
     1021 accatctttc agtgtttttt atcaaagcgt ttagtcggca taccagtgtc ctgcatatct
     1081 cttgtgcgtg ataacggagt gtaacaaaag ggacaggcat caagggattt tttgttatta
     1141 cagccgtcgg gtggcgttgt acacctgatc actgtttctg cgttttatga aaaaaataaa
     1201 attatgtatc ga
//
LOCUS       DAPGWD010000342         1200 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPGWD010000342 DAPGWD010000000
VERSION     DAPGWD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.346"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          305 a          303 c          274 g          318 t
ORIGIN      
        1 ccaagttggc atcggggatg aatgccatga actttcgtaa agaagattaa atcaatgtac
       61 atttgtgttg tttgctaatt gtgcataagt ctgccaccag atgaccagcc ccccaggatt
      121 agatgcagct atctgttagt aatgtcgcca ttccaccacc atgctgcaaa ctttgatggc
      181 atctgataat tcagtactga atgtggatga tactcgttat aattctgtcg ccagccataa
      241 tgactttcct tagcaaacga catccctgaa cctgtgctca ttcagacatt catcgcgaaa
      301 tagcccgtta aagcgctcag gaaatatgtt ctacgttagt ttgtccgact ggattctgtt
      361 caggtcttat cgtcaccgga tagccttgaa acagtgcaat gcagtccaga atacgcgtga
      421 cctgaacacc tgaaatcccg aatactgtgg ttattgttaa acactccttc gtaaatgtcg
      481 tccacgcagg tcaggcattt gattctgcga ccagtaacca gcgtgtccat ggcaaaatcc
      541 atcgacttgg tcaggttttg aacctccgga cagagttacg gcaagcgttc ttgaccagcc
      601 ctttacgacg tcatctgcat ttcacgccct ggcagctcag atgataaaga tggtaaacgt
      661 gcttgcgatt agcatgagcc ccttcacggt gcagtactac cagatgtggc ggttgccaaa
      721 acgtctgcgc tttagtgaca gcatcaatga tgtgtcctgc tcaatacgta tcagaatacg
      781 tattgagcag gactctgagt ctcatcgcgc agatcgacag ggacaagtca gcctgcagca
      841 agacgttgcg tcagagcagt cgtatcacat ataaaacggt gcctcgcttc gggtctgcca
      901 tcagtacttt agccctggag tcacctgaag cgcgtcctta tcgggatagc ttgggtaagc
      961 aacacctgga gtctgcgccc tcttcctcaa gcgacttcag gggcttgacc tcgggtatct
     1021 ccataccact atacttctta caccaggtat aaaagagtgc atcggacatg gatgcgcttg
     1081 ctacagagct cacgctcctc cggctatact gatactctgt ttgccggaaa aatgcttttt
     1141 catggggatg tcctcatgtg gactatgaag acattgctga caccaggatg catcaatcaa
//
LOCUS       DAPGWD010000343         1165 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPGWD010000343 DAPGWD010000000
VERSION     DAPGWD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.347"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..478)
                     /gene="sfmF"
                     /locus_tag="RHX88_004551"
     CDS             complement(<1..478)
                     /gene="sfmF"
                     /locus_tag="RHX88_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDV2233134.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGV"
     gene            complement(489..>1165)
                     /gene="fimH"
                     /locus_tag="RHX88_004552"
     CDS             complement(489..>1165)
                     /gene="fimH"
                     /locus_tag="RHX88_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDV2233135.1"
                     /translation="DSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRP
                     FINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGD
                     IGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQD
                     LGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRARPISITGQKPTEGPFSALGY
                     LRVDYQ"
BASE COUNT          319 a          308 c          267 g          271 t
ORIGIN      
        1 ccacacctgg ccgaacaccg ctggctaacg ctcgataatt ggcaaaaaag tttagtgtga
       61 cattgccatt tgcatctact tcctcagtcg ggctcgccgc tcccagtgcg agccgggagc
      121 gatcgctatt acgtaattcg atggcgatgg tttgcgccat tgcgggatca tccagagcca
      181 gcaggttggt atcggatgcc ggcgttcccg taaataaaat cgcaactgaa cccggaggac
      241 atccctccag ccgcaggcta aaagggacga gtgccgtggt atcgccagcg ttcagtaatt
      301 gtgtcgtagg ccatctgcct aaatctaccg tcttatcaat atccgcagtg tttacggtac
      361 aggagaaatc aacaacgtta ccgtgcaaat tgatattaat cgttcccaaa gggtcagctg
      421 cccatccact ggaaatccac agtaacccgc agaaacagct aaagagtgct cttctcattc
      481 tccgtacctc attgataatc gacgcgtaaa taccccagcg cgctaaacgg cccttcggtc
      541 ggtttttgac cggtaatact gataggcctg gcgcgaagtg tgacattggc tgccgcagct
      601 gcatccagac ggaaaggaat aacgctattg agatcgttag gcgtgatcgg cgtatcgttc
      661 tgatcggcga caataaaacc taaatcctga ttgtccgaca ccatcgcctg accagaaacg
      721 gcactggctt ccagacgcat tgttaaataa gcctgcgcag caacattcgt acatttgacc
      781 gcaatgctct tggtttgcgg catgacacca gcaggtcgat tacccggccc tgccgcacta
      841 aataacgatg cgccgatatc accaaaatca aattcaacaa tctgcccggc atttaattcg
      901 cagttttgcg gtacttcaac ccggccacca aaactaatgg tataaacagg tgtaaccaac
      961 ggatcgtaag gcgttgatgt cacatacacg gtaaacatca cctgtctggg gatctccacc
     1021 atgttaataa agggacgaat aacctttaaa cgaaatatta aacgagaatc catcaccccg
     1081 aatggaaggt tttgtgaaac gttaggatct gttcccatcc gaatataatt ttgcgggggg
     1141 aactgaatat ccatcacact atcaa
//
LOCUS       DAPGWD010000344         1162 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPGWD010000344 DAPGWD010000000
VERSION     DAPGWD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.348"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            170..472
                     /locus_tag="RHX88_004553"
     CDS             170..472
                     /locus_tag="RHX88_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2233136.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            519..941
                     /locus_tag="RHX88_004554"
     CDS             519..941
                     /locus_tag="RHX88_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDV2233137.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLPD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRVSRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            938..1129
                     /locus_tag="RHX88_004555"
     CDS             938..1129
                     /locus_tag="RHX88_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233138.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
BASE COUNT          270 a          277 c          365 g          250 t
ORIGIN      
        1 gcaccgcccg gtgtcgcgga atatgtgaac aacggaggaa aacatcatgc aatatgcgaa
       61 acctgtcact ctgaacgttg aagagtgcga ccgtctctcc tttctaccgt atctgtttgg
      121 caacgatttt ctgtatgccg aggcgtatgt gtacgcgctg gcgcaaaaaa tgatgccgga
      181 atatcaggga ggcttctggc acttcatccg cctgccggac ggtggcggtt acatgatgcc
      241 ggatggcgac cgtttccaca tggtgaacgg tgcaaactgg tttgaccgta ccgtgagtgc
      301 tgatgccgca ggcatcattc tgacttccct tgtgattaac cgccagttgt ggctgtacca
      361 cgacagcggt gatgcaggac tgacccagct ttaccggatg cgcgatgcgc agttgtggcg
      421 tcacatcgaa tttcaccctg aatgcaacgc gatttacgcg gctctggact gattaacgga
      481 cggggcggca atgccgcccc tgtaaaggag acgagaaaat gtactgtact gttaaagaaa
      541 ttatccgcga agtgctggat acagacgtgc cggacagtga atgcgttttt gccgtggtgc
      601 tgacccgtgg ggatgtgcgt cacatagccc aggactggaa cctgccagac gatgagctgg
      661 aaaccgtcat gcagcggctg gacgatgcct ttgagcatgg tgcggatgtc agcgttgttc
      721 acgacgttgt tcgtgaactg atggaagaaa agcgcgtcag ccgtcaggtg acagtcccgg
      781 cggtgatgct ggaaaaagtg atggcgctgg caggcagtga aatgaagcgc ctgtatgccg
      841 tcgggagtga gaacgggggc gacggtgacg cattcgtcag ggaagaacgc gaagcgatgg
      901 acgttgtgtt acaggcgctg gacggggagc acatgtcatg aatatcagca cagaaacccg
      961 cgaaattctg cgcaattaca aagccgtgat taatgcgcgg cgtcgtgaaa tggggcagaa
     1021 accgctcacc actgcgcaga ttgttgatga aatctgcgat tttgtggtga accagcaggc
     1081 ggttttcctc ggtggtcact atatccttca gggcagcaga aacaggtgat acagggcagg
     1141 cggtggaggc cgcctgtcag cc
//
LOCUS       DAPGWD010000345         1158 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPGWD010000345 DAPGWD010000000
VERSION     DAPGWD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.349"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            124..364
                     /locus_tag="RHX88_004556"
                     /pseudo
     CDS             124..364
                     /locus_tag="RHX88_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            384..671
                     /gene="sctI"
                     /locus_tag="RHX88_004557"
     CDS             384..671
                     /gene="sctI"
                     /locus_tag="RHX88_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDV2233139.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKEGLTPEKLLVLQDHVLNYNVEVSLVEH"
     gene            712..1158
                     /gene="sctJ"
                     /locus_tag="RHX88_004558"
                     /pseudo
     CDS             712..1158
                     /gene="sctJ"
                     /locus_tag="RHX88_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
BASE COUNT          378 a          200 c          241 g          339 t
ORIGIN      
        1 taagatttaa tgtgaatttg aaaaatcagg acacaaataa tagttcattt agctacggag
       61 ataaccgatt cgagaagtct caaggtagca actggacatt tcaggaataa ttgaggagga
      121 cttatggcag attggaatgg ttatattatg gatatcagca atcaatttga tcagggcgtt
      181 gatgatctga accagcaagt tgaaaaagcg ttggaggatt tagccaacca atccctccga
      241 cacgaaattt cttgccgaat atcaaagtgc attagctgag tatacattat atcgaaatgc
      301 gcaatctaac gttgtaaaag cgtataaaga tcttgattca gcaatcatac aaaacttccg
      361 ctaaactgga taaggatttt attatgtcag taagcaatat gccacctatt gatagagctg
      421 agcaatcaac tgctcatgaa atacagcaag cgaaagtgat tgatttaaat gaccgggttc
      481 tcaatcttga caacccggat gataaaatga tctccgcttt tgccaactat gctgtacaaa
      541 ctgaaaattg gcagcaaaat gcgttacaag cattaaggtc tgataaagag gggttaactc
      601 ctgaaaaatt attagtcttg caggatcatg tgcttaatta taatgtcgag gtttcattgg
      661 tggaacatta gcaagaaaga ttgtcgctgc agttgagacg ttgacccgct catgaaatat
      721 atatcattat tattgtttat cctgctgttg tgcggctgca agcagcagga attattgaac
      781 catcttgatc aacagcaagc taatgatgtc cttgctgttt tacaaagaca taatattaat
      841 gctgaaaaga aggattaagg caaaacagga ttttcaatat ttgtcgaacc aacggatttt
      901 gcttctgcgg tggattggct aaaaatctac aatctaccgg gtaagcctga tattcaaata
      961 tcgcagatgt tccctgcaga tgcactcgtt tcatctccac gagcggaaaa agcaaggcta
     1021 tattctgcta ttgagcagcg gttagagcaa tctctcaaaa ttatggatgg cattatttct
     1081 tcgagagtac atgttagtta cgatgttgat aatggcgata gtggtaaaac tgctttacca
     1141 attcatattt ctgtactt
//
LOCUS       DAPGWD010000346         1157 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPGWD010000346 DAPGWD010000000
VERSION     DAPGWD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.350"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..899)
                     /locus_tag="RHX88_004559"
     CDS             complement(<1..899)
                     /locus_tag="RHX88_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV2233140.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGR"
BASE COUNT          282 a          303 c          310 g          262 t
ORIGIN      
        1 cgtcccggcc atatctccgg cgagcgactc attccccgcg cggtttctgc attgtttcgc
       61 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cacccttgaa acgtccgggg
      121 tagtcataca cttcatactg cgtgcgctgg taatcctggt gctggccttc ctgttcaaaa
      181 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacgga agaagggcgg
      241 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
      301 ggattccagg ggatttcaaa agattcgggc agatggcgga ctgtgtcgca cagcaccagg
      361 ctctggtcgg tacttttgta agcatgctcc tcataaaaga agatgccttc ctccgccgcc
      421 atccggcaca gaaaatcgta atcagtctca ccgtactgga cacaaaactc acgggaagga
      481 tgcggctcgc tgaacagcgg actccattcg gtcaccccgt tttcctgcaa catcgtgccg
      541 aggatgcttt tgatgtcctc gttctggaag atacggaagt tctggcgcag accggcacgc
      601 cacagcggcg ggtgcacctt catgctgtac agcatctggt ttttgtcgtt ctcccccagt
      661 tcaaaccagg tcaccacacc tttcacccgg cgctgtacgt catcgccctg ccataccgtc
      721 aggtaggcca ttttgtccag cacctgcgca aattcaaggg aaagaaactg ctgactgacc
      781 agagaaaggt cgagggaaaa aagtgaagag agtgactggg tgagatgaaa ggaaaccacc
      841 gcaaaagcat ccggcggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
      901 gcaacctccg tgaaaatgaa taataatccg cacatattgc atgtacaaca tgtcgggcgg
      961 atgagataag cgagtcgaca tctggcgtta ccggggaata tcagaaaaat gtcatggtta
     1021 tgtgatgatt tagtctggga ttttacttca gagaaacggg tgtctgttta gtcaacaaaa
     1081 taatcacaaa cgcaacggat gggttctcga cgttattcac gacaggctcg tacattacag
     1141 cgagtaataa aaaacct
//
LOCUS       DAPGWD010000347         1141 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPGWD010000347 DAPGWD010000000
VERSION     DAPGWD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.351"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1141
                     /gene="gltS"
                     /locus_tag="RHX88_004560"
     CDS             <1..>1141
                     /gene="gltS"
                     /locus_tag="RHX88_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV2233141.1"
                     /translation="LLIGMFVVKFTPFLQKNHIPEAVVGGFIVAIVLLIIDKTSGYSF
                     TFDASLQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTILIAIQNTVGMSMAVM
                     MNESPFIGLIAGSITLTGGHGNAGAWGPILADKYDVTGAVELAMACATLGLVLGGLVG
                     GPVARHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETISMLIICIVVGGY
                     ISALFKDTFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSVALSLFLAMAL
                     MSLKFGQLASMAGPVLIIIAVQTVVMVLFACFVTFKMMGKDYDAVVISAGHCGFGMGA
                     TPTAIANMQTVTKAFGPSHKAFLVVPMVGAFIVDISNSILIKIFIE"
BASE COUNT          306 a          212 c          239 g          384 t
ORIGIN      
        1 ctactcatag gaatgtttgt cgtaaaattt accccgttcc tacaaaaaaa ccacatacca
       61 gaagccgtcg ttggtggctt tattgttgca attgttctgt taattattga taaaacatca
      121 ggttattcgt ttacttttga tgcttcattg caaagtttat taatgctcac attcttttcc
      181 tctatcgggc taagttctga cttttctcga ctgattaaag gaggaaagcc gttagttcta
      241 ttaactattg cagtaacgat cctaatcgcc attcaaaata ctgtcggcat gagtatggct
      301 gtcatgatga atgaaagtcc atttattggc ttaattgcag gttcaattac tctaacaggt
      361 ggtcatggta atgccggagc atggggccct attctcgctg ataaatatga tgtaacaggc
      421 gccgttgaat tagcgatggc ttgtgcaaca cttggattag tgttgggtgg tttagttggc
      481 ggccctgttg cccgtcatct tttgaaaaag gtctctattc ctaaaacaac cgagcaagag
      541 cgcgacacta tcgttgaagc ttttgagcaa ccaagcgtca aaagaaaaat caatgcaaat
      601 aacgttattg aaaccatttc aatgctgatt atctgtattg ttgttggtgg ctatatcagt
      661 gcattgttta aagatacttt tctgcaactg cctacttttg tctggtgttt atttgtcggt
      721 attattatcc gtaatacact gactcatgta tttaaacacg aagtgtttga gccgaccgtc
      781 gatgtattag gtagcgttgc tttatcgctt ttcttggcaa tggcgttaat gtcattaaaa
      841 tttggtcaat tggcaagcat ggcagggcca gtattaatta tcattgctgt acaaactgtt
      901 gtcatggtgc tatttgcctg ctttgtcacc ttcaaaatga tgggcaaaga ttatgatgct
      961 gtcgtgatca gcgcgggtca ctgtggcttt ggtatgggag caacaccaac agcaattgcg
     1021 aatatgcaaa cagtcacaaa agcatttgga ccatcacata aagctttcct tgtcgttcct
     1081 atggtcggtg cctttattgt tgatatttca aacagtatct taattaaaat atttattgaa
     1141 a
//
LOCUS       DAPGWD010000348         1130 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPGWD010000348 DAPGWD010000000
VERSION     DAPGWD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.352"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..357
                     /locus_tag="RHX88_004561"
     CDS             <1..357
                     /locus_tag="RHX88_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2233142.1"
                     /translation="RPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANR
                     HGLRWLHDVANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHP
                     LHHPLSIYDSFCRGVA"
     gene            357..>1130
                     /gene="istB"
                     /locus_tag="RHX88_004562"
     CDS             357..>1130
                     /gene="istB"
                     /locus_tag="RHX88_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HDV2233143.1"
                     /translation="MMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANP"
BASE COUNT          314 a          300 c          283 g          233 t
ORIGIN      
        1 cgccccttca gggcacagac taaaggtaag gtggaacgga tggtgcagta cacccgtaac
       61 agtttttaca tcccactaat gactcgcctg cgcccgatgg ggatcactgt cgatgttgaa
      121 acagccaacc gccacggtct gcgctggctg cacgatgtcg ctaaccaacg aaagcatgaa
      181 acaatccagg cacgtccctg cgatcgctgg ctcgaagagc agcagtccat gctggcactg
      241 cctccggaga aaaaagagta tgacgtgcat cttgatgaaa atctggtgaa cttcgacaaa
      301 caccccctgc atcatccact ctccatctac gactcattct gcagaggagt ggcgtgatga
      361 tggaactgca acatcaacga ctgatggtgc tcgccgggca gttgcaactg gaaagcctta
      421 taagcgcagc gcctgcgctg tcacaacagg cagtagacca ggaatggagt tatatggact
      481 tcctggagca tctgcttcat gaagaaaaac tggcacgtca tcaacgtaaa caggcgatgt
      541 atacccgaat ggcagccttc ccggcggtga aaacgttcga agagtatgac ttcacattcg
      601 ccaccggagc accgcagaag caactccagt cgttacgctc actcagcttc atagaacgta
      661 atgaaaatat cgtattactg gggccatcag gtgtggggaa aacccatctg gcaatagcga
      721 tgggctatga agcagtccgt gcaggtatca aagttcgctt cacaacagca gcagatctgt
      781 tacttcagtt atctacggca caacgtcagg gccgttataa aacgacgctt cagcgtggag
      841 taatggcccc ccgcctgctc atcattgatg aaataggcta tctgccgttc agtcaggaag
      901 aagcaaagct gttcttccag gtcatcgcta aacgttacga aaagagcgca atgatcctga
      961 catccaatct gccgttcggg cagtgggatc aaacgttcgc cggtgatgca gcactgacct
     1021 cagcgatgct ggaccgtatc ttacaccact cacatgtcgt tcaaatcaaa ggagaaagct
     1081 atcgactcag acagaaacga aaggccgggg ttatagcaga agctaatcct
//
LOCUS       DAPGWD010000349         1100 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPGWD010000349 DAPGWD010000000
VERSION     DAPGWD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.353"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            163..987
                     /gene="gadX"
                     /locus_tag="RHX88_004563"
     CDS             163..987
                     /gene="gadX"
                     /locus_tag="RHX88_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDV2233144.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRSLQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
BASE COUNT          319 a          210 c          233 g          338 t
ORIGIN      
        1 acgtttttgt attataaatc cagtcatcct gcccgtagaa catcacacat tatcatcctg
       61 ttctcccgct tcgtttaaat ttatttatca atcaatttga cttaagaggg cggcgtgcta
      121 cattaataaa cagtaatatg tttatgtaat attaagtcaa ctatgcaatc attacatggg
      181 aattgtctaa ttgcgtacgc aagacataaa tatattctca ccatggttaa tggtgaatat
      241 cgctatttta atggcggtga cctggttttt gcggatgcaa gccaaattcg agtagataag
      301 tgtgttgaaa attttgtatt agtatcaagg gatacgcttt cattatttct cccgatgctc
      361 aaggaggagg cattaaatct tcatgcacat aaaaaagttt cttcattact cgttcatcac
      421 tgtagcagag atattcctgt ttttcaggaa gttgcgcaac tatcgcagaa taagaatctt
      481 cgctatgcag aaatgctacg taaaagagca ttaatctttg cgttgttatc tgtttttctt
      541 gaggatgatc actttatacc gctgcttctg aacgttttac aaccgaacat gcgaacacga
      601 gtttgtacgg ttatcaataa taatatcgct catgagtgga cactagcccg aatcgccagc
      661 gagctgttga tgagtccaag tctgttaaag aaaaaattgc gcgaagaaga gacatcatat
      721 tcacagttgc ttactgagtg tagaatgcaa cgttctttgc aacttattgt tatacatggc
      781 ttttcaatta agcgagtcgc agtgtcctgt ggatatcaca gcgtgtcgta tttcatttac
      841 gtctttcgaa attattatgg gatgacgccc acagagtatc aggagcgatc ggcgcaggga
      901 ttgccgaacc gtgactcggc ggcaagtatt gttgcgcaag ggaattttta cggcactgac
      961 tgttctgcgg aaggaataag attatagagt tttactcaga cataaaaaaa acccggcata
     1021 ggggaccggg aagaggatag tctgccgtct ccagactaat aaaccgttat aacactccct
     1081 gttggcacgg gaaactttgt
//
LOCUS       DAPGWD010000350         1098 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPGWD010000350 DAPGWD010000000
VERSION     DAPGWD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.354"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..899)
                     /gene="tssI"
                     /locus_tag="RHX88_004564"
     CDS             complement(<1..899)
                     /gene="tssI"
                     /locus_tag="RHX88_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV2233145.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGR"
BASE COUNT          258 a          289 c          296 g          255 t
ORIGIN      
        1 cgtcccggcc atatctccgg cgaacggctt gttccgcgcg ccacttctgc gttgtttcgc
       61 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cacccttgaa acgtccgggg
      121 tagtcataca cttcatactg cgtgcgctgg taatcctggt actggccttc ctgatcaaaa
      181 cgccctggcc agccgggtcg tttaaaggtg tagtccttgg tcaccacgga agaagggcgg
      241 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
      301 gggttccatg ggatttcaaa agattcgggc agatggcgga ctgtgtcgca cagcaccagg
      361 ctctggtcgg tacttttgta agcatgctcc tcataaaaga agatgccttc ctccgccgcc
      421 atccggcaca ggaaatcgta atcagtctca ccgtactgga cacaaaactc acgggaagga
      481 tgcggctcgc tgaacagcgg actccattcg gtcaccccgt tttcctgcaa catcgtgccg
      541 aggatgcttt tgatgtcctc gttctggaag atacggaagt tctggcgcag accggcacgc
      601 cacagcggcg ggtgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
      661 tcaaaccagg tcaccacgcc tttcacccgg cgctgtactt catcgccctg ccatatcgtc
      721 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctggctgacc
      781 agggagagat cgagggaaaa gagcgaagag agtgactggt tcagatgaaa ggaaaccacc
      841 gcaaaagcat ccggcggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
      901 gcaacctccg gtaaaatgaa taatgatccg taccgtacaa ctgcggataa tgtgataagc
      961 gggacgactt cagacgttat tagggaagga acagaaaatg ctatggttat gtgagaattt
     1021 tgtttaaata ttactttaca gaaacgggtg tctgttcagt caaaaaaata atcacaagct
     1081 caacctcttg tttcttgg
//
LOCUS       DAPGWD010000351         1095 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPGWD010000351 DAPGWD010000000
VERSION     DAPGWD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.355"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..310)
                     /gene="trbJ"
                     /locus_tag="RHX88_004565"
     CDS             complement(<1..310)
                     /gene="trbJ"
                     /locus_tag="RHX88_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HDV2233146.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIVTGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLSLTFSVSVVFFVGAIVGWISVSEIYYSCMTGLNESS
                     E"
     gene            complement(240..785)
                     /gene="trbB"
                     /locus_tag="RHX88_004566"
     CDS             complement(240..785)
                     /gene="trbB"
                     /locus_tag="RHX88_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HDV2233147.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRDEIERLWNPQGMATQPAQPAA
                     GTSARTAKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FSYTLDGQGDTAFPEALPVPPEVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARVDTVLQMYGGKKGAK"
     gene            complement(772..1056)
                     /gene="traQ"
                     /locus_tag="RHX88_004567"
     CDS             complement(772..1056)
                     /gene="traQ"
                     /locus_tag="RHX88_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HDV2233148.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
BASE COUNT          281 a          307 c          274 g          233 t
ORIGIN      
        1 tttccgatga ttcattcaga ccggtcatgc agctgtaata tatttcagac accgaaatcc
       61 atcccacaat tgcccccaca aaaaacacaa cagaaacaga aaaggtaaga ctcagcgaaa
      121 cgacaatttc tacagggctg gcgggataaa aaagcccctg acggataaag gggatattca
      181 ggctgacgat tattcccgtc acgatgccgg atattccggc aatgagtaac accacctgct
      241 tatttcgcac ctttttttcc tccgtacatc tgcaaaacgg tatccacccg cgccataaaa
      301 ccggcggcat ccgttgcccc ctgtaaaagc ggtaatgcct caagcgtgtt gacgttgacc
      361 aggaaggtgg tcggtgtggc caccgggata ttcgggaaga aggtctgcat cacctccggt
      421 ggcaccggta atgcttcagg aaaggccgta tccccctgac catccagggt gtaggaaaaa
      481 acggaaaagc cgtactgctg tgccagctgt ttcagtaccg ggtcaaactg gtgacagtaa
      541 gggcaatgcc cctgcataaa cagcaccacc ttccagtcgg ccaggttaac ctgcctgcca
      601 ttactgaggc ggaaccagcg gggagccggc tttgctgtcc tggctgacgt gcctgctgcc
      661 ggttgtgcag gctgcgtagc cataccctgc ggattccaga gtcgctctat ttcatcccgg
      721 gtggaggcct gcacagcagc ggcgctcagc aacagggtga acagcagtga tttagtgaga
      781 gacatgtccg ccctcctgct gcccttccat catggcctgt tgcctggctt ccagtgcctc
      841 acgctcttcc cggctgacac gggcaatcca tgcatccagc acctggtatg cgccgaacag
      901 tacgagaatg gccgcaatca gctccgccag aaaaaaagcc atccagggtt tgctgtaaac
      961 aagacgggcg acgatatgaa accagacacc cagggaaaat acccacattc ccgtaatatc
     1021 aagccggggt aaagagaatc tgcgtttact tatcatgtta aactccggtt attatttaca
     1081 tgagagtatt catgt
//
LOCUS       DAPGWD010000352         1093 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPGWD010000352 DAPGWD010000000
VERSION     DAPGWD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.356"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..394
                     /locus_tag="RHX88_004568"
     CDS             <1..394
                     /locus_tag="RHX88_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV2233149.1"
                     /translation="WHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSPEGEFQK
                     WNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEETSLLEA
                     WKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(449..>1093)
                     /locus_tag="RHX88_004569"
     CDS             complement(449..>1093)
                     /locus_tag="RHX88_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV2233150.1"
                     /translation="TDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEH
                     LGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEP
                     YPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHN
                     SFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          305 a          229 c          265 g          294 t
ORIGIN      
        1 gtggcatctc gttgaagatc atcggggtaa aacggtttat gacgtggctt ccggcgacgc
       61 gttatttatt tctgaactcg gctcattacc ggaaaatgtc acctggttat ccccggaagg
      121 ggagtttcag aagtggaacg gcacagcctg ggtgaaggat acggaagcag aaaaactgtt
      181 ccggatccgg gaggcggaag aaacaaaaaa cagcctgatg caggtagcca gtgagcatat
      241 agcgccgctt caggatgctg tagatctgga aatcgcaacg gaggaagaaa cctcattgct
      301 ggaagcctgg aaaaaatatc gggtgttgct gaaccgtgtt gatacatcaa ctgcacctga
      361 tattgagtgg cctacgaacc ctgtcaggga gtaatcattg ggattatgcc gcagcacgtc
      421 ttaagcaaga acgtgctgcg gttggatgct attttttccc tgaagcggaa aacattacta
      481 cagtaccttg aaccttggtt ttaacattct cgaaatgctc tgagagtata tgtgttaagc
      541 cttcttcgga atcttttgtg tttgaaaaga tgcctttctg attgtaaatg cgcatcagtt
      601 tttgaccgaa gctattgtgc acaactccat cgccaagaat tgtggctccg tatagagttc
      661 catcgtcagt taaggcctgc gccgcattgc gtattacaca gctttttgta gatatatttc
      721 caggcaggca gtgaagaagg taaaacatgg aaatggaatc aaattgacca tgtaacgccg
      781 cgggataagg ttcaaaaaca tcatggctaa ttttatgttt aatttttgat tccccagccc
      841 ttgtagatgc cgcgttcagg ctagcttcgt tcaaatccat taaagatatc agactactct
      901 caggtacgtg agtaaggtaa aacccagttc caacaccaat atccagatgg ttgttaccta
      961 aatgttccag aaagtgtgga agaaggtgtt cctttgtagg acatccccat gcaagccgat
     1021 ttgatactcc caaaacccac cagtcataaa gctttagggt aagtggtgtg taaattttag
     1081 ccccatcatc tgt
//
LOCUS       DAPGWD010000353         1092 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPGWD010000353 DAPGWD010000000
VERSION     DAPGWD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.357"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1092
                     /locus_tag="RHX88_004570"
     CDS             <1..>1092
                     /locus_tag="RHX88_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV2233151.1"
                     /translation="AARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPKKKDSPNYG
                     NPVNPVLGAKVLPGETDIALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDIRL
                     QIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQVL
                     PEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGRTL
                     AFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSASS
                     SLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYD
                     RSGTQVRGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT          185 a          322 c          397 g          188 t
ORIGIN      
        1 gcggcgcggc agggcgacat gacccgcaag ggactggata ttgtgcaggg ttcagcaggg
       61 gtgctgatag gcgcgccgac gggggttgcc tgctcggtgt gtcctaaaaa aaaggacagt
      121 ccaaattatg gcaacccggt gaacccggtg ctgggtgcaa aggtgctgcc gggcgagacg
      181 gacattgcgc tgcccggccc gctgccgttt attctttccc gcgcctacag cagctaccgg
      241 accagaacac ccgcgccggt gggggtgttt ggtcccggct ggaaagcgcc cttcgatatc
      301 cgcttacaga tacgcgatga aggcctgata ctcaacgaca gcggcggcag gagcattcac
      361 tttgagccgc tgtttcccgg cgagataagc tacagccgca gcgagtcact ctggctggcg
      421 cggggcggag tagcggcgca gcacagttcg cagccgctaa gcgcgctctg gcaggtgctg
      481 ccggaagatg ttcgcctgag tccgcatgtg tacctggcga caaacagcct tcaggggccg
      541 tggtggatac tcagctggcc ggagcgggtg ccgggggcgg acgaggtgct gccgccggag
      601 ccgcccgcat accgggtgct gacgggcgtg gtggacggct tcgggcggac gctggccttt
      661 caccgggcgg cggagggtga tgtggcgggc gcggtgacgg gggtgacgga cggcgcgggc
      721 cgccgttttc acctggtgct gaccacacag gcacagcggg cggaagtatt ccgtaaacag
      781 cgcgccacgt ctttatcttc ccctgccggt ccccgttctg cttcctcttc tctggttttc
      841 cccgacacgc tgcccgccgg tacaggatac ggcacggata acggtatccg gctggaggcg
      901 gtatggctga cacacgaccc ggcatacccg gatgaacagc cgactgcgcc gctggcgcgc
      961 tacacgtaca cggccggtgg tgaactgcgg gcggtgtatg accgcagcgg aacgcaggtg
     1021 cgcgggttta cttatgatgc ggagcacgcc gggcggatgg tggcgcacca ttatgcgggg
     1081 aggccggaga gc
//
LOCUS       DAPGWD010000354         1070 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPGWD010000354 DAPGWD010000000
VERSION     DAPGWD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.358"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..97
                     /locus_tag="RHX88_004571"
     CDS             <1..97
                     /locus_tag="RHX88_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDV2233152.1"
                     /translation="KKTGKEVTSIQFTAREKMTGLESYDIKIDLI"
     gene            393..599
                     /locus_tag="RHX88_004572"
     CDS             393..599
                     /locus_tag="RHX88_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233153.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
BASE COUNT          305 a          217 c          232 g          316 t
ORIGIN      
        1 gaaaaagacc gggaaagaag ttaccagtat ccagtttact gcacgggaaa aaatgactgg
       61 tcttgaaagc tatgatatca agattgattt aatctagcat gtttatagta ttagcatcaa
      121 tttctcatca gatgctattc aacaatacaa attacccata aacctcgttt ttacggggtt
      181 ttgttatatt taaacattac cgaatagata taaatctcga ttgtcgatgg tattggttat
      241 gtgacaatac ctagtctttc cggtatgcct gaagagaata caaacgacag attatgtaag
      301 ggaaacgcgc tgaaactttc actctttcat gctgagtgat ttgacgttat atttttagcc
      361 gcaagtgaag agcaaacgaa tggagcgaca aaatgcataa agatcaatac actgatgccc
      421 cctcccagga gcaggttcgt gtaaaaacta tgcttaatag caccatttcg atgggttacc
      481 cggatgttgt aattgcatgt atagaacatc aagtatctct ggaagcattc agggcaattg
      541 aggccgcgtt agtaaagcac gataagaata cgaaggatta ttccctggtg gttgactgat
      601 caccataact gccaaccatt ctgaccattt cacaagtgac agagtcaata tcgtattctg
      661 tcactgtaag gctaatacag tactgcaatt caactacagc aatgcctcgt atttaggtga
      721 atttacaata tcgtccagtt cggatgccgg ctgcatttct gaagataagg catttcggtt
      781 cgcgtatttt cccctcatgc tcgtcagtcc tgcgcgtagt aagaaacagg acactcacac
      841 taatttgtgt gggcatgctg tgatgtcctt ctgaattatc cccatgccat tatgtaaagc
      901 tctatttcag atgctcgtca cggttgtcag gctgtcgggt cctcccagtg ggggtccctg
      961 ccacggggcg ggagcgtcgc ggaaaaaggc tagtttttgc atttccatgg cggcggcagc
     1021 atgtttggta atttattgat aattaaaagt tatttctctt ttcacctgta
//
LOCUS       DAPGWD010000355         1065 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPGWD010000355 DAPGWD010000000
VERSION     DAPGWD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.359"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>1065)
                     /locus_tag="RHX88_004573"
     CDS             complement(<1..>1065)
                     /locus_tag="RHX88_004573"
                     /inference="COORDINATES: protein
                     motif:HMM:NF027494.1,HMM:TIGR04415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV2233154.1"
                     /translation="SSSMSLSAGDSAFNTTIDGGEQYVSSGGSAISTTINDGAQIIFN
                     GGTASTTTINGGYQDISSGGSVTNTLISGGDQYISDGGSATNTVIDGGTQYISSGGNA
                     IETTIENGFQEVSNGGSATSTVINAGFQYISSGGSATSTIINAGFQDVSSGGSATSTT
                     INGGFQLISSGGSAVDTTIQGGIQEVGEGGRASGTTINNSGIQFISSGGSATATTINS
                     GGWQEISSGGSATETAINGGIQWIYDGGSASDSTINSGYQVISSGGSVTSTTIYRGGE
                     QSIHNAGLATGTIISGGEQLVSSGGSAVDTTIEGGLQTILNGGNVSGTLISGGVQRVS
                     SGGSAVDTTVEEGLQTVLNGG"
BASE COUNT          264 a          304 c          221 g          276 t
ORIGIN      
        1 ccgccattga gaacagtctg aagcccctcc tccacggtgg tgtctacagc actaccaccg
       61 ctggaaactc gctgcactcc tccgctaatg agtgtccctg atacatttcc gccattgaga
      121 atggtctgca gccccccctc aatggttgta tctacggcac taccaccgct ggagactagc
      181 tgctccccgc cactgatgat ggttccggtc gcaagacctg cattgtgaat actctgctcc
      241 ccaccacggt aaattgtggt agaagttaca ctgccgccgc tggagatgac ctggtaacca
      301 ctgttgatgg tactatctga agctgagcct ccatcgtaaa tccactgaat gcctccattt
      361 atggccgtct cagttgcaga gccaccactg gatatttcct gccaaccgcc actattaata
      421 gtggttgctg tagcacttcc tccactgctg atgaactgga taccagaatt atttatagtg
      481 gtaccacttg cacgtccccc ctcaccaact tcctgaatcc cgccttgaat agtggtgtca
      541 acagcactgc caccgctgga aatgagctgg aagccaccat taatagtggt gctggtagca
      601 ctgccaccac tggaaacatc ttggaatccg gcattaataa tggtgctggt agcactacca
      661 ccacttgaaa tgtactggaa tccggcatta ataacggtac tagtagcact gccaccattg
      721 gaaacctcct ggaacccatt ttcaatagtg gtttcaatag cattgcctcc actggatata
      781 tattgagttc ccccatcgat aacggtatta gtcgcactgc caccatcgga aatatactga
      841 tcccctccgc taattagtgt attagtgaca cttccaccac tcgaaatatc ttggtaacca
      901 ccattaatgg tcgtcgttga agcggtacca ccattgaaaa ttatctgtgc cccatcattg
      961 atagtcgtgc ttatagcgct gccaccactg gatacgtatt gctctccgcc atcaatggtg
     1021 gtattaaatg cggaatcacc tgctgataaa gacattgagc tacta
//
LOCUS       DAPGWD010000356         1052 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPGWD010000356 DAPGWD010000000
VERSION     DAPGWD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.360"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            53..838
                     /locus_tag="RHX88_004574"
     CDS             53..838
                     /locus_tag="RHX88_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV2233155.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          347 a          205 c          215 g          285 t
ORIGIN      
        1 ttatcaatgc taaatattct aatcattatg acaggcgagg gagtgtccaa ttatgaattc
       61 aaaaaagctt tgttgcatat gtgtgttatt ctcgctgctt gcaggatgtg cctctgaatc
      121 ttctattgat gaaaagaaga aaaaagctca agtcacacaa agtaatatta ataaaaacac
      181 tccccagcaa ctgacagaca aagatttatt cggtaatgaa accactctgg ccgtatccga
      241 agaggatatt caagctgcgc ttgatggaga tgagttccgc gttcccctta attctcctgt
      301 aatacttgtt caatccggca accgcgcacc ggaaaccatt atgcaggagg agatgcgaaa
      361 atattatact gtttccacat tctctggtat cccggacagg caaaagcctc tgacttgtaa
      421 caaaaacaaa gataaaaatg agaacgagga tgttgccagt gctgagaata tgaactggat
      481 gcaggcactg cgttttgtgg ctgcaaaagg acatcagaaa gcgattattg tttaccagga
      541 tacattgcag acaggaaaat atgactctgc gctgaaatca acagtatggt ccgactataa
      601 aaatgaaaaa ctcaccgacg ctatctccct gcgctacctg gtacgtttca cgctggtgga
      661 tgtggcaaca ggtgagtggg ctacctggtc gccggtgaat tatgaatata aagtgcttcc
      721 accattgccc gacaagaatg aagccagtac gactgatatg acagagcagc aaatcatgca
      781 acttaagcaa aagacctata aagcgatggt aaaagatttg gttaaccgct atcaataata
      841 aattatatct gccgccagga atttctggcg gcaataatac aaaattcttt gcataagaaa
      901 tttttcttga caaagacagt gcaagaaagg acatggaatc cggagttaaa aactgtttgt
      961 tttagaatat ttaattattt aactgctgaa tcttccttgc gagaaatata ttcattatta
     1021 catttcatgc catttttaat atagattgct cc
//
LOCUS       DAPGWD010000357         1041 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPGWD010000357 DAPGWD010000000
VERSION     DAPGWD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.361"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            35..904
                     /locus_tag="RHX88_004575"
     CDS             35..904
                     /locus_tag="RHX88_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDV2233156.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT          312 a          231 c          241 g          257 t
ORIGIN      
        1 ttatttaatt gagaaatggt tagggagaac ctacatggcc ataagcatca agggtgttaa
       61 taccggtgtt attcgcaaga gtaataattt cattgcactg gcgctgaaaa ttaaagaacc
      121 tcgcaataaa gaatcgttgt tctttatgtc tgtcatggaa ctgcgcgacc tgctgattgc
      181 gctggaaagt cgtatgcatc aaaagcataa actggatgct gctgcacgcc ttcagtatga
      241 gcaagctcgc gataaagtca ttaagaaaat ggcagaaaac atcccagaaa ttctggttga
      301 tgagcttaaa aatgccgata ttaatcgtcg ggttaacacg ctggaactta ccgataatca
      361 aggcgaaaat cttaccttcg tattaacgct acatgacggt agcaaatgtg agttagtcgt
      421 caatgagttg cagattgaaa tgctggcgcg agcaatcatt catgcaatta ataatgcgga
      481 aatgcgtgaa ctggcattac gtattacctc actgttagat ttcctgccgt tatatgacgt
      541 tgactgccag gaaaatggca atctggaata tgacacctat tcccaaccgg aatggaaaca
      601 taacctgttc gaccattatc tggcggtgct ttaccgcttt aaagatgaaa gtggcaaaga
      661 acaatttagc ggcgcagtgg tgaaaacgcg tgaagcaacg ccgggtaaag aaatagaggc
      721 gatcactcgc cgaatgcttg atttcagccc acgactgaaa aaactcgcgg gtgtgccatg
      781 tcaggtctat gttcgcaccg tagcggcgaa taacgcccaa ccgctaaccc aggatcaatg
      841 cttacgcgcg ctgcatcact tacgtgttca atccaccagc aaaactgcac cacaggcgaa
      901 ataatctcat ctttgctaat gcgaggctgg agccatccag cctgaaatga ctgataacgt
      961 gatcgttggt catgccggat gcggcgtaaa cgccttatcc ggcctacaaa ttctcaagca
     1021 ttcaatcagt tgcaatgcct g
//
LOCUS       DAPGWD010000358         1030 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPGWD010000358 DAPGWD010000000
VERSION     DAPGWD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.362"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..830)
                     /locus_tag="RHX88_004576"
                     /pseudo
     CDS             complement(1..830)
                     /locus_tag="RHX88_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            897..>1030
                     /locus_tag="RHX88_004577"
     CDS             897..>1030
                     /locus_tag="RHX88_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2233157.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     "
BASE COUNT          287 a          207 c          201 g          335 t
ORIGIN      
        1 tccccacgac taaaggccag aacgttttta gttgcagata caccctcaaa tgcgctccat
       61 attcctgctt tatctatata taatgcgtgg aattcattgt agagctgctg ataggtagag
      121 caacctttat taagctacgc ctcaccgccg acacgcgtgg acccatcata atttttaatt
      181 tcagtcgttg attttaatac ctggccatcc cagtaaacta aaggtctgct gaacgggaga
      241 gcggctatat atgccattgc taattgttca tcttgttgat ggttaaatag aaagtcattg
      301 aaaagattac cattgttagg aaaatcgtgg ttaatgttaa tcaatatccg tctttctgat
      361 gggatacttg agattgtgga acccggtttt gatgcaaggg tttgaagatc tccaccaaat
      421 gaaaaggctt ttatcgtttt catgagcaat gaaaaatcaa tggcgcttaa atcatctctt
      481 tcataaagca gtgatgacat ttgttgttca tccaactgac aagttaccag atattccatg
      541 taattcataa cggctttttt atgatatttt gggttaaata agttagtatt gtgtaaccgc
      601 tcattataat ttttaagcgg tggggttatt gatctttcta tttgttcatg ttttgagtgt
      661 tttgccgcat cataacgtaa acctcgtacg ccatattttc gcatttcacg aacgtattga
      721 ttttgcaaca taataacctg gtcacttttg ggtgttgttt taggcattcc atttaaacgc
      781 ttttctttta atgcatggag gttttcccat tcatccttag agatgttatc ttgatcttac
      841 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacatga
      901 caaaaacagt atcaaccagt aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg
      961 aagccctgaa gcttgctgaa cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt
     1021 atgaatcaca
//
LOCUS       DAPGWD010000359         1029 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPGWD010000359 DAPGWD010000000
VERSION     DAPGWD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.363"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(220..411)
                     /locus_tag="RHX88_004578"
     CDS             complement(220..411)
                     /locus_tag="RHX88_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233158.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(408..830)
                     /locus_tag="RHX88_004579"
     CDS             complement(408..830)
                     /locus_tag="RHX88_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDV2233159.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(877..>1029)
                     /locus_tag="RHX88_004580"
     CDS             complement(877..>1029)
                     /locus_tag="RHX88_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2233160.1"
                     /translation="LTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNA
                     IYAALD"
BASE COUNT          229 a          330 c          245 g          225 t
ORIGIN      
        1 caacactgca gtagtcagaa tcagagcagc agataaagga acaacaacat caggaacagt
       61 tccataacat aagaaagaaa cactcctgag tcagacacat cacagacggg aagggtaacg
      121 ggcgacgcgc cagcgtggcc cgcttccctt ccggcaggcg tcagcctgcc aggggttttc
      181 gcgcggactg acaggcggcc tccaccgcct gcccctgtat cacctgtttc tgctaccctg
      241 taagatgtag tgaccgccca ggaaaaccgc ctgctgattc gccacaaaat cgcagatttc
      301 atcaacaatc tgcgcggtgg tgagcggttt ctgccccatt tcacgacgcc gcgcattaat
      361 cacggccctg taattacgca gaatttcgcg ggtttctgtg ctgatattca tgacatcgtc
      421 tccccgtcca gcgcctgtaa cacaacgtcc attgcttcgc gttcctccct gacgaatgcg
      481 tcaccgtcgc ccccgttctc actcccgacg gcatacaggc gcttcatttc actgcctgcc
      541 agcgccatca ctttttccag catcaccgcc gggactgtca cctgacggct ggcgcgcttt
      601 tcttccatca gttcacgaac aacgccgtga acaacgctga catccgcacc atattcaaag
      661 gcatcgtcca gccgctgcat gacggtttcc agctcatcgt ctgtcagact ccagtcctgg
      721 gctatgtgac gcacatcccc acgggtcagc actacggcaa aaacgcattc actgtccggc
      781 acgtctgtat ccagcacttc gcggataatt tctttaacag tacagtacat gttctcgtct
      841 cctttacagg ggcggcattg ccgccccgtc cgttaatcag tccagagccg cgtaaatcgc
      901 gttgcattcg gggtgaaatt cgatgtgacg ccacaactgc gcatcgcgca tccggtaaag
      961 ctgggtcagt cctgcatccc cgctgtcgtg gtacagccac aactggcggt taatcacaag
     1021 ggaggttag
//
LOCUS       DAPGWD010000360          998 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPGWD010000360 DAPGWD010000000
VERSION     DAPGWD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.364"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..269
                     /locus_tag="RHX88_004581"
     CDS             3..269
                     /locus_tag="RHX88_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HDV2233161.1"
                     /translation="MIITIALISLLSACTRENSLKENQNKRSGESQAIVDARQFFSEH
                     PEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(330..692)
                     /locus_tag="RHX88_004582"
     CDS             complement(330..692)
                     /locus_tag="RHX88_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV2233162.1"
                     /translation="MIATPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIG
                     IPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPVPDEQLSSEDK
                     FIEGEDTKIENKDDNLKN"
     gene            complement(719..>998)
                     /locus_tag="RHX88_004583"
     CDS             complement(719..>998)
                     /locus_tag="RHX88_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV2233163.1"
                     /translation="WSNHKSLLFKTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIF
                     DFIAEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERIRKFFLSN"
BASE COUNT          316 a          189 c          162 g          331 t
ORIGIN      
        1 acatcataat aaccatcgcg ctgatatcgt tgctctctgc atgcaccaga gaaaattcat
       61 taaaggagaa tcaaaataaa agaagtggcg aatcccaagc gatagtagat gcaagacagt
      121 tctttagcga acacccggaa tatatatcat cagcagaact ggagcaaaag ctatatcgcg
      181 aatttgcaca ggttactaca gttccagctt ataaagagat gagtatgtat caattgctta
      241 tcatttcaca agatcgttta actcattaaa taaggatcta cacgcttttt gcgtgtagat
      301 tcttataaat ttctgactac gacagatgtt taatttttta aattatcgtc tttattctcg
      361 atttttgtgt cttcaccctc aatgaattta tcttctgaag agagctgttc atctggaaca
      421 ggttgtccag cattctccac atcttcaagc cacagcagaa gacgtaatat ttcatctata
      481 ttttcgaagc taacataatc gtagcgacga tgggtagcat aaatttttcg tgctaatttt
      541 ttatctgtga taattggtat tccgatttcc tttgcatatt tccttacggc taaggctacc
      601 tcattagttt ctcttacaga aatcaatgga ataggtgaaa gatgtggctt aaagtatatc
      661 cctattgcaa tgtgagtagg ggtggcaatc atcaaacgac tattactgac atcagacttc
      721 aattgctcag aaagaatttc ctgattcgct ctctgcgttt agacttaatt tctggatttc
      781 cttcttgctc cttgtattct cttttaactt cttgtttatc cattttcata tctttcataa
      841 ataaaaaata ttcagcgata aaatcaaaaa ttaagacaat tatcatactg ccgagacaat
      901 acagtatgag aaggaatagc atctctcccc aatctgataa taaagatatg atatctccat
      961 caagagtttt aaaaagcagt gatttatgat tactccaa
//
LOCUS       DAPGWD010000361          994 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPGWD010000361 DAPGWD010000000
VERSION     DAPGWD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.365"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..738
                     /locus_tag="RHX88_004584"
     CDS             <1..738
                     /locus_tag="RHX88_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233164.1"
                     /translation="INLDSAFSLSEEERSYFEEKAKSLNALAQLGISVEIISHELEEM
                     DSMVTRGLNSLPTSVKEHPGFSLALNAHRSLTQQIRFLSPLKISGYQSRQRITGKNIM
                     DYVLKFFGERFERQRITIEFSEEFKQIAITDIPSRIYPVFTNIINNAMYWVSLSNNRL
                     IKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRANGHGVGLYLCRENLAVAHHKIW
                     YSEPDEGDNYLIKDGANFVIQFNGVEF"
     gene            740..>994
                     /locus_tag="RHX88_004585"
     CDS             740..>994
                     /locus_tag="RHX88_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233165.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIR"
BASE COUNT          323 a          162 c          192 g          317 t
ORIGIN      
        1 ataaacttag attcagcatt ctcattatct gaagaagaac gctcatattt tgaagaaaaa
       61 gctaaaagtt taaacgcgct tgcacagtta ggtattagtg ttgagataat atctcatgaa
      121 cttgaagaaa tggattctat ggtaaccaga ggactaaact ctcttcctac ttctgtaaaa
      181 gaacaccctg gtttttcatt ggcgttaaat gctcacagat cgcttactca acaaatacgt
      241 ttcttatcac ctttgaaaat atcaggttat caatccaggc agagaataac tggaaaaaat
      301 atcatggatt atgtcctgaa gttctttggg gagcgtttcg aacggcaacg aataactatt
      361 gaatttagtg aagagtttaa gcaaatcgca ataacagata taccatcaag gatctatcct
      421 gtttttacta atattatcaa caatgcaatg tattgggtca gtctgtcaaa taataggctc
      481 ataaagattg gttttgtgaa ttctttggtt atcatagcaa attctggtcc ggcaattgat
      541 accgatgata tcccgcgact atttgaacta ttttatagca aaagagcaaa tggacatggg
      601 gtaggtctgt atctatgtcg agaaaacctt gctgttgcac atcataaaat atggtattca
      661 gaacctgatg aaggcgataa ctatttaata aaagatggcg ctaattttgt gatccagttc
      721 aatggagtgg agttctaata tgacagtggc aaattataat tctcttgtcc agaaaacttt
      781 ctgcgaaaat gcaattcgtt ccgttgtcat gattgatgac gattttctga cgtattctga
      841 atcaatcagg gcgttgaata acgaagttga tttagactac aacaaaattg actcatctaa
      901 acgagccgct actcttgaga gcttttttca atctaaaaat atgatttgtg atgttgacaa
      961 tggttctgtt aatttcgatg tggatcggat taga
//
LOCUS       DAPGWD010000362          983 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPGWD010000362 DAPGWD010000000
VERSION     DAPGWD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.366"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..546)
                     /gene="sctT"
                     /locus_tag="RHX88_004586"
     CDS             complement(<1..546)
                     /gene="sctT"
                     /locus_tag="RHX88_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDV2233166.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVCH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTI"
     gene            complement(548..808)
                     /locus_tag="RHX88_004587"
     CDS             complement(548..808)
                     /locus_tag="RHX88_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV2233167.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(818..>983)
                     /locus_tag="RHX88_004588"
     CDS             complement(818..>983)
                     /locus_tag="RHX88_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV2233168.1"
                     /translation="DLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLI
                     LQYFDVSINP"
BASE COUNT          346 a          212 c          165 g          260 t
ORIGIN      
        1 gatagtatga gtcattatgt tattaatata tgagaataaa ggctctaatt taggaaaatt
       61 aaaattgcct gaaggccata aattataact ttgccaaaga gtttccagaa taaagttcat
      121 tccaccttta gtaagataaa ccgcagcaga gaaaaggtta aataatcttg ctaattccga
      181 ggtatcgaca cctgtagccg gatccaaagt actacttaat gttgcaccgc gttggttatc
      241 gataaagcta cctatagcaa ggaaaatcca aaacggggat gctaataagc atgcaataaa
      301 cagccctatg ataatttctc tggcagcgaa caaagcaata tcgatttcat ttaagttcaa
      361 taattcatag tggtaatgct gaactaatgc acatgatacc actattatta ttgggtgaca
      421 aactactgtt ggtatattcc cactggcgaa aaatggtaac aaatagaaag ttggtgccaa
      481 tcggcaaaaa ccgagcgctg tagcagccag cagactatgc aactgataga gtatcgcctc
      541 ccccatgcta tcctctcgcg aatgctaaat tcagcatttc gataccaaat gaatataatt
      601 tttcaccata ccacccgctc attaaaaaaa agcaaatact tacgcacaat aatttcacgc
      661 caaatggcaa cgtctgttct tgcaactgcg ttaccgtttg aaaaagccct accaataaac
      721 caacaaatgt cgcaactgca ataggtcctg cagacataac taaaatcaga tacagcgccc
      781 tattccccgc aaaaacaata tcgtccatat tttttcctca gggattaatg gatacgtcaa
      841 aatattgtaa aatgagccct tttgataaca ttgtccaacc gtccatcgcg acaaacaaaa
      901 ttaattttat cggtgtagat atggttaccg gactcatcat catcatacct aacgttaaca
      961 gaacgctgga tataaccaga tcc
//
LOCUS       DAPGWD010000363          974 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPGWD010000363 DAPGWD010000000
VERSION     DAPGWD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.367"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>974
                     /locus_tag="RHX88_004589"
     CDS             <1..>974
                     /locus_tag="RHX88_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter strand-loop-strand
                     O-heptosyltransferase"
                     /protein_id="HDV2233169.1"
                     /translation="SGPEGIRYDFNDGARVLLPEGKWHVRLMDADSGNILFSCDADNG
                     WVRSCKKYFIRFRIQVFHRGNDTPLMDETLNLKNQPVLISFPTGTLGDLLGWFPYAER
                     FQTLHKCQLECTMAQEIIELLAPQYPQIQFSTPDNPHTITPYATYRVGLYFGGDTTNQ
                     PIDFRQVGFHRSAGYILGVDPRESPVRLNLSAPRSINEPYVCIATQSTCQAKYWNNGT
                     GWSEVVAHLKSLGYRVLCIDLHAHYGQGFVWNHIPWGAEDFTGKIPLQERVNLLHHAS
                     FFIGLSSGLSWLAWATHIPVVLISGFTLPVSEFYTPWRIFSSHGCNGC"
BASE COUNT          242 a          203 c          236 g          293 t
ORIGIN      
        1 tccggacctg agggtatccg atatgatttt aatgatggtg cgcgtgtact gctccctgag
       61 gggaaatggc atgttcgtct gatggatgct gattcgggga acattctttt tagctgcgat
      121 gcagataatg gctgggtaag gagctgtaaa aaatacttca ttcgttttcg tattcaggta
      181 ttccataggg gaaacgatac gcccttaatg gatgaaacgt tgaatttaaa aaatcagcct
      241 gtgctgattt cgtttcctac cggtacatta ggtgatttac tcggatggtt tccttatgct
      301 gaaaggtttc agacgctgca taagtgtcaa ctggaatgca ctatggcaca ggagattatt
      361 gaactactgg caccgcaata tccgcagatt caattttcta ctccagataa tcctcatacg
      421 attacgccat atgcaactta tcgtgtagga ctgtattttg gtggcgatac aactaaccag
      481 ccaattgatt ttcgccaggt cggttttcat cgttccgctg gatacattct gggagtggat
      541 ccccgagaat caccagtccg acttaatctt tcagctccca gaagcattaa tgaaccttat
      601 gtatgtatcg cgacacaatc cacttgtcag gcgaaatact ggaacaacgg cacaggctgg
      661 agtgaggttg tagcccatct caaatcgctg gggtatcggg tactgtgtat tgatctgcat
      721 gctcattatg gtcagggctt tgtctggaac catattccgt ggggagcaga agactttaca
      781 gggaaaatac ctctgcagga acgggtgaat ctgttacatc atgccagttt ctttattggt
      841 ttatccagcg ggctctcctg gctggcatgg gcaactcata tccctgttgt actaataagt
      901 ggttttacgc ttcctgtcag tgaattttat actccatgga gaatatttag ttcccatgga
      961 tgtaacggat gctg
//
LOCUS       DAPGWD010000364          965 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPGWD010000364 DAPGWD010000000
VERSION     DAPGWD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.368"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..>965
                     /locus_tag="RHX88_004590"
     CDS             49..>965
                     /locus_tag="RHX88_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HDV2233170.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLKTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHS"
BASE COUNT          289 a          229 c          252 g          195 t
ORIGIN      
        1 taagcagtta attcatttgt tttcctcaga ggatgaagga gataccgaat gtctgaccct
       61 gtacgtatta caaatcccgg tgcagaatcg ctgggatatg attcagatgg ccatgaaatt
      121 atggccgttg atatttatgt aaaccctcca cgtgtcgatg tctttcatgg taccccgcct
      181 gcatggagtt ccttcgggaa caaaaccatc tggggtggaa acgagtgggt cgatgattcc
      241 ccaacccgaa gtgatatcga aaaaagggac aaggaaatca cagcgtacaa aaacacgctc
      301 agcgcgcagc agaaagagaa tgagaataag cgtactgaag ctggaaaacg cctttctgcg
      361 gcaattgctg caagggaaaa agatgaaaac acactgaaaa cactccgtgc cggaaacgca
      421 gatgccgctg atattacacg acaggagttc agactcctgc aggcagagct gagagaatac
      481 ggattccgta ctgaaatcgc cggatatgat gccctccggc tgcatacaga gagccggatg
      541 ctgtttgctg atgctgattc tcttcgtata tctccccgcg aggccaggtc gttaatcgaa
      601 caggctgaaa aacggcagaa ggatgcgcag aacgcagaca agaaggccgc tgatatgctt
      661 gctgaatacg agcgcagaaa aggtattctg gacacgcggt tgtcagagct ggaaaaaaat
      721 ggcggggcag cccttgccgt tcttgatgca caacaggccc gtctgctcgg gcagcagaca
      781 cggaatgaca gggccatttc agaggcccgg aataaactca gttcggtgac ggaatcgctt
      841 aagacggccc gtaatgcatt aaccagagct gaacaacagc tgacgcaaca gaaaaacacg
      901 cctgacggca aaacgatagt ttcccctgaa aaattcccgg ggcgttcatc aacaaatcat
      961 tctat
//
LOCUS       DAPGWD010000365          944 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPGWD010000365 DAPGWD010000000
VERSION     DAPGWD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.369"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>944
                     /locus_tag="RHX88_004591"
     CDS             <1..>944
                     /locus_tag="RHX88_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV2233171.1"
                     /translation="ATECIAADVILDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVA
                     RGGNQIRMIGGEYGALWRNDGAKTYLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTK
                     LSASLNGNALTATKLQTPRLVSGVEFDGSKDITLTAAHVAAFARRATDTYADADGGVP
                     WNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDW
                     AEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPD
                     MRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLG"
BASE COUNT          238 a          211 c          253 g          242 t
ORIGIN      
        1 gcgactgaat gtatcgcggc ggacgttatc cttgatttta agagtgggcg tggtttttat
       61 gagtctcatt cgttaatcgt taacgataac ttgtcgtgca aaaaactttt tgccacagac
      121 gaaattgtag cgcgtggtgg taatcagatt cgaatgatag gtggggagta tggtgcatta
      181 tggcgtaatg atggcgctaa aacttacctg ctgcttacca atcaaggtga tgtttatggt
      241 ggctggaata cattaagacc gtttgctatt gataacgcaa ccggcgaact ggttattgga
      301 accaaactgt ccgcaagtct gaacggtaat gcattaacag caacaaagct gcaaacgcca
      361 agactggttt ctggtgttga gtttgatggt tccaaagata ttactttaac cgccgcgcat
      421 gtggctgctt ttgccagaag ggcaacggat acgtatgccg atgcggatgg gggcgttcca
      481 tggaatgccg aatcaggcgc ttacaatgtc acccgctctg gcgacagcta tattctggtt
      541 aacttctata ccggagtcgg aagttgccgg accttgcaga tgaaggcaca ttacagaaat
      601 ggaggtctgt tctaccgttc ctcaagagat ggctatggtt ttgaggaaga ctgggcagaa
      661 gtttatacct cgaaaaatct tccaccagaa agctacccag tcggcgcacc aatcccgtgg
      721 ccatcagata ccgttccgtc tggttatgcc ctgatgcagg ggcagacttt tgacaaatct
      781 gcatatccga aacttgcagc cgcttatccg tcaggcgtga tccctgatat gcgtggctgg
      841 acgattaagg gcaaacctgc cagtggtcgg gccgtattgt ctcaggaaca ggacggcatt
      901 aaatcgcaca cccacagcgc cagcgcatcc agtacggatt tggg
//
LOCUS       DAPGWD010000366          911 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPGWD010000366 DAPGWD010000000
VERSION     DAPGWD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.370"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            66..698
                     /locus_tag="RHX88_004592"
     CDS             66..698
                     /locus_tag="RHX88_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2233172.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMAGKINELFIRSHSVQH"
BASE COUNT          301 a          155 c          173 g          282 t
ORIGIN      
        1 gtgtattgac atcatcaatc accaatgaaa ttatgcaatt atatacggat agggaggttc
       61 ttaacatggg gctttgtagt cgttataaaa gtcttacatg caatagttgc tcaatgcatt
      121 gccaaataat gccagaagag tcaccgcgtt tgcagtactg cgcaaactcg tgtttttgta
      181 tgtggcccga agaaagttca tattttaatc gtggtgtggt agaaggtatc ttaacaaaaa
      241 accacaatgc gagattaagc ggatatattt ttgtcgattt ttcagtaagt tttttacgtc
      301 tattccttga aaaagactgg attgactatc ttgcaagtac tgacatgggg attgttttag
      361 tcagcgaccg caatatgcaa tcgttagcta attactggcg aaaacacaac tcagcaatat
      421 ctgctgtcat atataatgat gatggtcttg atgttgccaa tgaaaagatc agacaactat
      481 ttattggtcg ttatttatca tttaccagag gtaacacatt aactcagatg gaattcacca
      541 taatggggta tatggtttct ggttataatc catatcagat tgctgaagtt ctggatatgg
      601 atatccgtag catctacgcg tacaagcaac gaatcgaaaa gagaatggct ggtaaaataa
      661 acgaattatt tattcgttca cattcggtcc aacattgata ctcaacaatc tgcaagaatc
      721 atactctgtt tatacgggca tttatggaaa tgcccgtatt ttataaatag gccgtcacct
      781 caagagtaaa cacatagtat ttaaaaccat cacacgaaaa attcagaagc attacgaaat
      841 atgaacttac aaacaatagt ggcataaatg ttaaccatgt taatttacat aaagttttac
      901 gttgcgacat t
//
LOCUS       DAPGWD010000367          896 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPGWD010000367 DAPGWD010000000
VERSION     DAPGWD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.371"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..779
                     /gene="yeeO"
                     /locus_tag="RHX88_004593"
     CDS             <1..779
                     /gene="yeeO"
                     /locus_tag="RHX88_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDV2233173.1"
                     /translation="AVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASV
                     ESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGV
                     GQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNAL
                     FMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGM
                     FADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
BASE COUNT          184 a          178 c          255 g          279 t
ORIGIN      
        1 gcgcagtggc aattttgtgg gtgctggcga ttggttttaa tcctgcgcta aggatttcgt
       61 taaagagcta ttttaaaccg ctgaatttta gcattatctg ggaagtcatg gggattggta
      121 ttcccgcgag tgtcgaatca gtgttattta ccagtggtcg gttattaacc caaatgttcg
      181 ttgccgggat ggggaccagt gttattgccg gaaattttat cgcgttttca attgcggctc
      241 ttatcaactt acccggaagt gcgctcggct ctgcttctac gatcattaca ggccgaaggt
      301 tgggtgtagg acagatagcg caagcagaga ttcagttgcg gcatgtgttc tggctttcca
      361 ctcttggatt aacggccatc gcctggctaa cggctccctt tgccggggtt atggcatcgt
      421 tttacaccca ggatccacag gttaaacatg tcgttgtgat tctgatttgg ctaaatgctt
      481 tatttatgcc tatttggtct gcctcatggg tgctacccgc tggatttaag ggtgctcgcg
      541 atgcacgtta cgccatgtgg gtttctatgt tgagcatgtg gggttgccgg gttgtagtcg
      601 gttatgtgct gggaatcatg cttggctggg gtgtggttgg tgtctggatg ggaatgtttg
      661 ccgactgggc tgtgcgggcc gtgctgtttt actggcgaat ggttactgga cgttggctgt
      721 ggaaatatcc tcgacctgag ccgcaaaagt gtgaaaaaaa gccagttgtg tcggaataaa
      781 cgacaaagtg cagattattt cagcaaacga tttcaaattt aaaaaacagg ctttgacatt
      841 gtggatgagc gtcgctaata ttcgcctcgt tctcacgatt cctctgtagt tcagtc
//
LOCUS       DAPGWD010000368          883 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPGWD010000368 DAPGWD010000000
VERSION     DAPGWD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.372"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            25..687
                     /locus_tag="RHX88_004594"
     CDS             25..687
                     /locus_tag="RHX88_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HDV2233174.1"
                     /translation="MSTAIKKRNLEVKTQMSETIWLEPASERTVFLQIKNTSDKDMSG
                     LQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKADKMDLRESQGWLNRGYEGAAVGAA
                     LGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVEDVNYTMITDVQIAERTKATVTTD
                     NVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNANKVNLKFEEAKPVLEDQLAKSIAN
                     IL"
BASE COUNT          235 a          196 c          249 g          203 t
ORIGIN      
        1 ctggcccttt cagggtgtgg tgcgatgagc acagcaatca agaagcgtaa ccttgaggtg
       61 aagactcaga tgagtgagac catctggctt gaacccgcca gcgaacgcac ggtatttctg
      121 cagatcaaaa acacgtctga taaagacatg agtgggctgc agggcaaaat tgctgatgct
      181 gtgaaagcaa aagggtatca ggtggtgact tctccggata aagcctacta ctggattcag
      241 gcgaatgtgc tgaaggccga taagatggat ctgcgggagt ctcagggatg gctgaaccgt
      301 ggttatgaag gcgcagcagt tggtgcagcg ttaggtgccg gtatcaccgg atataattcc
      361 aattctgccg gtgccacact cggtgtgggc cttgctgccg gtctggtggg tatggctgca
      421 gatgcgatgg tggaagatgt gaactatacc atgatcacgg atgtgcagat tgcagagcgt
      481 actaaggcaa cggtgacaac ggataatgtt gccgccctgc gtcagggcac atcaggtgcg
      541 aaaattcaga ccagtacaga aacaggtaac cagcataaat accagacccg tgtggtctca
      601 aatgcgaaca aggttaacct gaaatttgaa gaggcgaagc ctgttctcga agaccagctg
      661 gccaaatcaa tcgcaaatat tctctgatta tcttccggag gctggtctga ccggcctcct
      721 gactgacgcc tcgctttgct cgttgtccaa ccccggaaag aaataaaata aattttattt
      781 ctccccggcg tttccgaaat aaatatgaaa acactgagcg gttgtgcaaa ttctttgtgc
      841 gaccgtgaag tgttgccggc gaagccggaa tttaacggtt att
//
LOCUS       DAPGWD010000369          857 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPGWD010000369 DAPGWD010000000
VERSION     DAPGWD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.373"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(65..736)
                     /gene="queE"
                     /locus_tag="RHX88_004595"
     CDS             complement(65..736)
                     /gene="queE"
                     /locus_tag="RHX88_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDV2233175.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGSASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          223 a          217 c          189 g          228 t
ORIGIN      
        1 aacaatataa atgagtagaa gtctttacag gttccaccca tttataacgc ttataaatgt
       61 ttaatcaggc aatatttaga tatttatgtg tttgcatcga caaacgccaa ttacgcgcaa
      121 tgcaggtttc aatgcacaaa cgtgtggcat catccttttg gctaatcggc tgcagtgcaa
      181 tgactcgcgg tttatcatcg gtcagcgttg ccaatagttc atccagtgct tcaatatcgc
      241 gtacgcgccc cactggatgc ttgatttcgt tggctcgctc cagtgcctgt gacaacacgt
      301 catagccgcc gcgcatgttc agctttggcg atacggtaac ccaggtattc ggtgtgcagc
      361 gtacctcatg agtaccactg gtttcgatct ggcagctaaa accgttcttt tcgagcagat
      421 cagtcagtgg tagcaaatca tgaatgcaag gctcaccacc cgtaatcacc acatgccgcg
      481 cggtgtatcc ctggcgacca atgacagcca gcaaatcttc actgctcgcc gacccccact
      541 tatcactctc tttggtcttc gccagaatgc tgaaaaggga gacttcccga tcctcaagct
      601 tttcccaggt gtgtttggtg tcgcaccagg cacagccaac cgggcatccc tgtaaacgaa
      661 taaaaatggc gggaacgccg gtaaagtaac cctcaccttg cagggtctgg aacatctcgt
      721 taatcgggta ctgcatagca ttctctgtga agtggataat tgttaattat tgcagatcct
      781 gccgcaacaa tcatgtctta ttaacattct gttacaggca ggtttaaggc aaaaagtgca
      841 ttagatcata aagaata
//
LOCUS       DAPGWD010000370          843 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPGWD010000370 DAPGWD010000000
VERSION     DAPGWD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.374"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            152..736
                     /locus_tag="RHX88_004596"
     CDS             152..736
                     /locus_tag="RHX88_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2233176.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          247 a          162 c          170 g          264 t
ORIGIN      
        1 acaataggcg tcctaatgaa caatggttgt aaattttggt aacaaatatc attgctcatt
       61 aatatctagc atctgcttat aacatttgtg tcatgaatag tcattcttca aaccagtagt
      121 ctgactaatg gaattttatt caaggatttt tatgtctaaa aaattaggtt ttgccctgtc
      181 tggtcttatg ctggcaatgg ttgcaggtac tgcttctgct gatatggatg gtggtcagtt
      241 aaatatcagt ggtctggttg ttgataacac ctgtgaaacc cgcgttgacg gcggtaataa
      301 agatggtctg atcctgctgc aaactgcaac tgttggtgaa atcgatgctg gcgtgctgaa
      361 tgacaccgtt ggcgctaaag ccaaaccttt cagcatcacc gttgactgca gcaaagcaaa
      421 tccgaatcca ggtagtacag ctaaaatgac cttcggttct gttttctttg gtaatagcaa
      481 aggaacgctg aacaacgaca tgtctattaa caacccgtct gacggcgtga atatcgctct
      541 gcacaatatt gatggttcca ctatcaaaca ggttcaaatt aacaaccctg gcgatgtgta
      601 tactaaagcc ctggatgcaa cgacaaaatc tgctgtttat gattttaaag cgtcttacgt
      661 tcgtgccgtc gcagaccaaa cagcaactgc tggttatgta aaaactaaca ctgcatacac
      721 cattacttat cagtaagtat tcaccaatta aattgattga atgaatatac agggaatagt
      781 gttaataatt tctattttta tattattccc tgttttaatt aactctgtca gggatggttt
      841 atg
//
LOCUS       DAPGWD010000371          840 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPGWD010000371 DAPGWD010000000
VERSION     DAPGWD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.375"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>840
                     /locus_tag="RHX88_004597"
     CDS             <1..>840
                     /locus_tag="RHX88_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233177.1"
                     /translation="PGGLSETDNLFLKDVMSGKGPEKTFSFHLDNVNNIVERLKKLGV
                     LQRDNAQHPAVIRWLIDNDPDTLKNNIMALLSQTGSQRVVSLLMLMQNDFTTYVRLRY
                     LEIFMSDEHILNRLLAHLCASEERTPEQKFFVQEIAAHLLCLTEKSNIWQSVEINKRI
                     GELIDSSPILITAVPKGYGDAFFEVLKDNTLSVSYIPGDVGDEKCSVIRKIAGAGLFK
                     YSVSNLKNVYLCLTQDKNEERMSFSLYPFHCLESLAISELTEVLWTNIEDFILSVFIE
                     SEE"
BASE COUNT          248 a          143 c          185 g          264 t
ORIGIN      
        1 ttcccggagg gctgagtgag acagataatt tatttcttaa ggatgttatg tctggtaaag
       61 gtccggaaaa aacattctca ttccatcttg ataacgttaa taatattgtt gaacgactca
      121 aaaagctggg ggttctgcag cgtgacaatg ctcaacatcc tgctgttatc agatggctga
      181 ttgataatga ccctgatacc ctgaaaaaca atataatggc attactgagt cagacgggta
      241 gccagcgtgt ggttagtttg ctgatgttga tgcagaacga tttcacaacg tatgttcgcc
      301 tgcgttacct ggagattttt atgtcagatg aacatatact gaacagattg ctggcacatt
      361 tatgtgcgtc agaagaacgc acacccgagc aaaagttttt tgttcaggaa atagcggcac
      421 acttgttatg cctgactgaa aaatcaaata tctggcaatc ggttgagatt aataaacgta
      481 tcggtgaact tatagattcc tccccaattc ttattactgc tgtgccaaaa ggatatggtg
      541 atgcgttttt tgaagtgttg aaagataata cactttcagt ttcatatatt ccaggtgatg
      601 tgggagacga gaagtgttct gttatcagga aaattgcggg tgcaggatta ttcaaatatt
      661 ccgtcagtaa tcttaaaaat gtttatcttt gcctgacgca agacaagaat gaagaaagaa
      721 tgtcattctc tctttatccg tttcattgtc tcgagtccct ggctatttct gaattaacag
      781 aagttctgtg gactaacata gaagatttta ttttatcggt atttattgaa tcggaagaga
//
LOCUS       DAPGWD010000372          835 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.376, whole genome shotgun sequence.
ACCESSION   DAPGWD010000372 DAPGWD010000000
VERSION     DAPGWD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.376"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..368
                     /locus_tag="RHX88_004598"
     CDS             <1..368
                     /locus_tag="RHX88_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV2233178.1"
                     /translation="INDKWGHHMGDQVIVMVTRIIKKSIRKEDMLGRLGGEEFGIIIK
                     GNTQKLLLSIAERIRKNIEEQCSEKLLSHGPEKITVSIGCFTSKENNLSPSEMLVNAD
                     KALYQAKRTGKNKVIIHSK"
     gene            complement(442..>835)
                     /locus_tag="RHX88_004599"
     CDS             complement(442..>835)
                     /locus_tag="RHX88_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV2233179.1"
                     /translation="WHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSPGGKYQK
                     WNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEEETSLLEA
                     WKKYRVLLNRVNTTTAPDIEWPTVPIIE"
BASE COUNT          255 a          190 c          165 g          225 t
ORIGIN      
        1 gcatcaatga caaatggggg catcatatgg gtgatcaagt catagtaatg gttaccagaa
       61 taataaaaaa atccatcagg aaagaggata tgttagggcg cttaggcggt gaggagttcg
      121 gtattatcat taaaggtaat actcaaaagc tcttgctatc aattgcagag cgaatcagaa
      181 aaaacattga agagcaatgc tcggaaaaat tattatcgca tggacctgag aaaataactg
      241 tcagtattgg ttgctttact tcaaaagaga ataatctcag tccatctgaa atgttagtca
      301 atgccgataa agcgttatat caagccaaaa gaaccggaaa aaacaaggtg ataattcact
      361 caaaataaac acctttttaa aatacagccc caataaactg cagaatatta tcccatataa
      421 tatcctgcag ttcgtaatgc actattcgat aatgggtact gttggccatt caatatccgg
      481 tgcagttgtt gtattaacac ggttcagcaa cacccgatac ttcttccagg cttccagcaa
      541 cgaggtttct tcctccgttg caatttccag atctgcagca tcctgaagcg gcgcaatatg
      601 ctcactggct acctgcatca ggctgttttt tgtttcttcc gcctcccgga tccggaacag
      661 tttttctgct tctgcatctt tcacccaggc tgtgccgttc cacttctgat atttccctcc
      721 cggcgacaac caggtaacat tttccggtaa tgagccgagt tcagaaataa ataacgcgtc
      781 gccggaagcc acgtcataaa ccgttttacc ccgatgatct tcaacgagat gccac
//
LOCUS       DAPGWD010000373          828 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPGWD010000373 DAPGWD010000000
VERSION     DAPGWD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.377"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..619
                     /gene="ompP"
                     /locus_tag="RHX88_004600"
     CDS             <1..619
                     /gene="ompP"
                     /locus_tag="RHX88_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpP"
                     /protein_id="HDV2233180.1"
                     /translation="TPGTWTDESRHPDTRLNYANEFDLNVKGWFLKESDYRLAIMAGY
                     QESRYSFNATGGTYIYSENGGFRNETGALPDKIKVIGYKQHFKIPYVGLTGNYRYDNF
                     EFGGAFKYSGWVRGSDNDEHYVRQTTFRSKVKNQNFYSVAVNAGYYITPEAKVYIEGV
                     WSRLTNKKGDTSLYDRSDNTSEHNNNGAGIENYNFITTAGLKYTF"
BASE COUNT          254 a          145 c          188 g          241 t
ORIGIN      
        1 gactcccgga acatggacag atgaaagcag gcatcctgat acacgtctta attatgccaa
       61 cgaatttgat ttgaacgtta aaggctggtt tttaaaagaa tctgattatc gccttgctat
      121 tatggcaggt tatcaggaaa gccgttacag ttttaatgct acaggaggaa cttatattta
      181 cagtgagaat ggtggtttcc ggaatgaaac gggagcgtta cctgataaaa taaaagtgat
      241 tggttataaa caacatttta aaattcctta tgtcggtctg acaggaaact accgttacga
      301 taattttgag tttggtggtg catttaaata cagcgggtgg gtcaggggat ctgataatga
      361 tgagcattat gtaagacaaa ctacattccg aagcaaagta aaaaaccaga atttctattc
      421 tgttgcagtt aatgccggtt actatatcac cccagaggca aaagtgtaca tcgagggtgt
      481 atggagtcgt ctcacaaata aaaaagggga tacatctctt tacgaccgta gtgataatac
      541 ttcagagcat aataataacg gggctggaat tgaaaattac aacttcatta cgacggccgg
      601 tctgaagtac acgttttaac aactttaact gtcatcatcc ccggtgagaa ttataccgcc
      661 ggggattctg tctgttggtc agggtaataa tttaccaatg caggagatgc aatgcgtgtg
      721 ataatgaagc ctttaagaag gactttagta ttctttatct tttctgtttt tctttgcgga
      781 actgtgtctg ccagacagat cgagtggcaa tcctgtatga ccagccca
//
LOCUS       DAPGWD010000374          809 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPGWD010000374 DAPGWD010000000
VERSION     DAPGWD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.378"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            18..479
                     /locus_tag="RHX88_004601"
     CDS             18..479
                     /locus_tag="RHX88_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233181.1"
                     /translation="MSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITG
                     LSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRK
                     AVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLKQLTRICMR"
     gene            647..>809
                     /locus_tag="RHX88_004602"
     CDS             647..>809
                     /locus_tag="RHX88_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233182.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQ"
BASE COUNT          300 a          134 c          141 g          234 t
ORIGIN      
        1 ttctagcgga atgccatatg tctttggcac ttcatggaaa aagtgaactt gaacttgctg
       61 ctcaaaaagc attagagctt ttagattatg tatcagacat aaccactgtc gatggaaaaa
      121 ttttagctat tatgggactg ataactggtc tgtctggaca agcaaaagta tctcatatct
      181 tatttgaaca ggctaagata cactcaactg atatagcctc tctctactac tatagggcac
      241 tagtccactt tcataatgaa aaaattgaag aggcaaggat ttgtatagac aaatcactac
      301 aactcgaacc cagaagacga aaagcagttg tgataaaaga atgtgtagat atgtatgtgc
      361 ctaacccgct caaaaacaac attaaactct actataaaga aactgagagt gaaagccatc
      421 gagttataat tgacaacatt ttgaaattaa agcagctgac gagaatttgt atgcgataaa
      481 tttttattga cgcaatttta ttatttagat gtatgcaact tgttatttaa acaacagaga
      541 taaaaatatc taagatattc accttattgc aagatattta aaatgctcta gagttaaaag
      601 tatgatgtaa agaccattgg cctggacaat taaaaaaggg agctttatga ctaatccaat
      661 cggtatcaat aatttatctc aaagttcaaa tatagcgaat gcaacgggtg atgaagtcgt
      721 atcattagat aaacacatca acaccagtgc tactgataca gatcaaatac aagcatttat
      781 agtttcaaca tggatggcgc cttttcaaa
//
LOCUS       DAPGWD010000375          806 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPGWD010000375 DAPGWD010000000
VERSION     DAPGWD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.379"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            8..>806
                     /locus_tag="RHX88_004603"
     CDS             8..>806
                     /locus_tag="RHX88_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2233183.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKL"
BASE COUNT          254 a          175 c          145 g          232 t
ORIGIN      
        1 gtagtgtatg gccaatctct acgacttgaa aaagttcgac ttaaaccttc ttgtcatatt
       61 tgagtgtatt taccagcatt taagcattag caaagctgct gaatcactat atatcacccc
      121 atcagccgtg agccagtcgt tacaacgctt acgtgcgcaa tttaatgacc ctttattcat
      181 tcgctcagga aaaggtatcg cgccgacgac aacagggttg aatctgcatc accatcttga
      241 gaaaaacctc aaaggccttg aacaaacaat taatatagtc aataagtccg aactaaagaa
      301 aaactttatt atttacggcc ctcagctcat ttcttgctct aataatagta tgctgatccg
      361 ctgcctacgg caagatgcct ctgtcgagat tgagtgccat gatatcctca tgtcagcaga
      421 gaatgctgaa gaactgttgg ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt
      481 gatcagccgt tccgtcattt gcatgccatt gcatacaatt cgcaatactc taatttgtag
      541 taataagcat ccgcgaatta ctgacaatag cacttatgaa caaattatgg cagaagagtt
      601 cacccaatta atatccaaat cagcaggagt tgatgatatt caaatggaaa tagacgagaa
      661 gtttatgaat cgcaaaattt catttcgtgg ttcctctttg ctgacaatca ttaatagcat
      721 tgcagttaca gatttactgg gaatagtccc ctatgaactt tataattcct accgtgactt
      781 tcttaattta aaagagataa aactag
//
LOCUS       DAPGWD010000376          802 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.380, whole genome shotgun sequence.
ACCESSION   DAPGWD010000376 DAPGWD010000000
VERSION     DAPGWD010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.380"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            145..>802
                     /locus_tag="RHX88_004604"
     CDS             145..>802
                     /locus_tag="RHX88_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV2233184.1"
                     /translation="MLSCLSPTLVCAEQPHGFIDYRHEYLDDTRTHADRVEFGTFFSN
                     GIGLMGELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRFKPLDDKKFWLEPMFWLDTTQ
                     YWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRNNRRYDVWIDYRPQ
                     KTNFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKMGSWIPYMSIADYKGVDKTSSN
                     R"
BASE COUNT          263 a          143 c          160 g          236 t
ORIGIN      
        1 taaaatttaa agtcaaacta tagataaaaa aacattctaa tacctggaat catcagatgg
       61 tgtattcgat caccaaaaat atattttatt ctgaaaggga atattatgaa ttcttatctt
      121 aaaatatcac ttgcaggaat aacaatgtta tcctgcctgt cacctacact agtctgtgca
      181 gaacagccgc atggatttat tgattatcgt catgagtatc ttgatgatac tcgcacccac
      241 gcggaccgcg ttgaatttgg tacatttttt agtaatggta tcggtttgat gggagaatta
      301 cgctataaca ccgatgaagg tgataaagat aagtgggacc cctcacaatt tggcaataac
      361 ggaacaggat tatctgttgt ttaccgcttt aagccgctgg atgacaaaaa attctggtta
      421 gaacccatgt tctggcttga taccacgcaa tattggtcaa cttacgaata tggtctttct
      481 gcgggttatg atttcagcaa agaatggaaa gtttcgggtc gttttcgtta tgacatggat
      541 aaagctaccg ataaatccaa aggttatggc aacgatgatc gtaataaccg ccgttacgat
      601 gtgtggatcg actatcgacc acaaaaaacc aacttccaat atcagttaaa cctggtctat
      661 tacaataatg gttatttaac ctggaatgat ggccataaaa actataccgc atcatttaaa
      721 gtagggtaca aaatgggatc gtggataccc tatatgagta ttgcggatta taaaggtgtg
      781 gataaaacat caagtaatcg cc
//
LOCUS       DAPGWD010000377          801 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.381, whole genome shotgun sequence.
ACCESSION   DAPGWD010000377 DAPGWD010000000
VERSION     DAPGWD010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.381"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(168..731)
                     /gene="ipbA"
                     /locus_tag="RHX88_004605"
                     /gene_synonym="fimX"
     CDS             complement(168..731)
                     /gene="ipbA"
                     /locus_tag="RHX88_004605"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HDV2233185.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          245 a          155 c          162 g          239 t
ORIGIN      
        1 atcactggta tgatctttgt ggttaatcat ttcaaatcat ggggttattt tactttagcg
       61 agaaatatca acatgttatg tgttttattt aattgaatgg gctgtttttt tatggaagaa
      121 ttgaggaaaa cgtaggaatc tgacattttg aatcagaagg tactgtttta acgaggcttt
      181 tttttccaca cccctttaaa acgagcagca ttactggctg tgtatctgac agtatgctga
      241 atatttcgat gacccagata atcctgaagt agtcgggtat cgacaccatt gtcagccaga
      301 gcaaaaccac aggcatgacg taacatatga ggatgtgctt ttacagctaa tcctgcacgt
      361 tttccagcct cacgaatgat agaaaaaaaa tgttgtctgc tgatagggcg gcgtttccgg
      421 gagagaaaaa gccagtctcc ttcaactccg ttttcaatta acttcctctg cttaagccac
      481 aacttgatca aattatattc atcaggaaga agaggatgtg ttgtcgagaa accattttta
      541 atacgccgaa tattaagttg ttttccagat gcatcaatat cggataaacg taaatcaaga
      601 agttcactgg ccctaaaacc gtgaatgaaa gccatcataa ttagacagcg atttctttca
      661 ggaaaggaca tactctgggc tgcatccatc agcctgtaga cttcatcctg tgtgagatat
      721 tttctcgtca tacgccaccg tgtacagttt taaaaaaaag agaggcacac ccagataagt
      781 atgcctgcaa tcagaacgtt a
//
LOCUS       DAPGWD010000378          795 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.382, whole genome shotgun sequence.
ACCESSION   DAPGWD010000378 DAPGWD010000000
VERSION     DAPGWD010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.382"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            46..>795
                     /locus_tag="RHX88_004606"
     CDS             46..>795
                     /locus_tag="RHX88_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2233186.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNY"
BASE COUNT          263 a          153 c          151 g          228 t
ORIGIN      
        1 aaactatgtg atttccattt cgattgattt agtgtttatt gacgtatgta ctggattatt
       61 aacgataata tcgagttctg gcctgagcac cgaaaattaa tatcggtaca taacgccgat
      121 cttaacgtcg ttctgacaac gccagccagt cgttgtttat cacttctact tgaagctttt
      181 cctgatgtgg ttgcacaaca agattttttc accagagtct gggaagaaga aggtatgcgt
      241 gtgcctacta acacgttata tcagaacata tccattatca gacgcggatt tcgcgctgtt
      301 ggtgatacta cccactcgct tattgcaacc gtaccgagaa gaggattcaa gatccataat
      361 gacattaaca tacaaaatca tgtaataaac tcatcaacag acgcacatac acacaatgcc
      421 ccacctgcca taaaagttaa tgcgggatac aaagagagca ttggtggcgc aaagaatttc
      481 aataacaaaa tcctcaaaca tataaaaagt tatctaatta tgttgagcgc atttgtcata
      541 ggcgcatatt ctgcatattg gctatggaat aataatcaac cgaaaccatt tttcaaggat
      601 tacaagactg ttgcggaaat taatggctgc cattttaatg taacagaaga tacaattgat
      661 gggttgaaag agttcgataa atataaaaca cggatactgg attcggggat taactgtaaa
      721 aaatatccgt ggttatactt ccctcttgca aaatcctcac cggggatgat tgttatggca
      781 tgcaataaaa actat
//
LOCUS       DAPGWD010000379          776 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.383, whole genome shotgun sequence.
ACCESSION   DAPGWD010000379 DAPGWD010000000
VERSION     DAPGWD010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.383"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(230..772)
                     /gene="fimA"
                     /locus_tag="RHX88_004607"
     CDS             complement(230..772)
                     /gene="fimA"
                     /locus_tag="RHX88_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV2233187.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          237 a          159 c          160 g          220 t
ORIGIN      
        1 tagtcatcat agttaatacg ccttgtgtat tcctgacggc aaaaataaga aatataaaga
       61 aatgattgct ttggtttctt acaacacatc attccctttt catttaataa atcggcatga
      121 tacaaagtga acatgatttc gatttacatt gccaccatac cgcttcagac aaccggtaca
      181 ttactttgtt ataagacgtg atatataatt caaaacaacg tggttttgat tattcgtatc
      241 tcataataaa agtcgcgtca gcatttgctt gccctgctga tgcacttgta ccggtgcctt
      301 tataacgtgc agaaaaatga agaacgttgg tcccggggat caagttctgg ttggttgaga
      361 agctattccc atcaggtttg agaattgcgg atgtattatc aagtatttca ataccgacac
      421 ccgttgctgt tgttgtattg gtactggatg caatggccaa taaattatca ttgattgcat
      481 cagcctgacc agaaaatgca acggcagcat tagcggcaac aacaggatcg cagtcgttca
      541 actgaatggt gaatggaatt aatgcagagg tattaccaac agcattgaaa atatcggtac
      601 gatattgacc gagtgtgaca acctggtctg ccgagtcagt attcactgaa caggcagcat
      661 tcaccagttc gccttcaaag tgaattgtac cgccatctac aacggcagca taactacccg
      721 tagcggcgaa tagtgcggca gccagcgcag acgaaataaa tcttaatttc atatat
//
LOCUS       DAPGWD010000380          768 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.384, whole genome shotgun sequence.
ACCESSION   DAPGWD010000380 DAPGWD010000000
VERSION     DAPGWD010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.384"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>768)
                     /locus_tag="RHX88_004608"
     CDS             complement(<1..>768)
                     /locus_tag="RHX88_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV2233188.1"
                     /translation="ENVFPALDIFTSQFANSKPYYSVSEASSKIQYALDLNGVIDKMK
                     MQDLLLFEGPIQVIQRLYSVGFIGLYNQQSSSYVFCHDGKEPEKEFTPDSKLLLHPCY
                     WLALSVHESEMTPDAADDIHDEYDIEVSSVSEEQRKQRIGSLLQELHNIPEGPEGAVD
                     FEAWALKTIKLLFATNLTNIELHPNKNGLQQRDIIATNIADTPVWKRILTDYQTRQVV
                     FEVKNYKTLGAAEYRQVNSYLFKDYGRIAFIINRDHT"
BASE COUNT          228 a          157 c          131 g          252 t
ORIGIN      
        1 ctgtgtgatc tctatttatg ataaaagcta tccgcccata gtctttaaac aaataggaat
       61 ttacctgtct atattctgca gcacctaatg ttttatagtt tttcacttca aaaacaactt
      121 gccgtgtctg ataatctgtc agaattctct tccaaactgg agtgtcagct atatttgtcg
      181 ctataatatc tctttgctgc aacccatttt tattgggatg caactcaatg ttcgttaaat
      241 tcgtagcaaa tagtaacttt atagttttga gcgcccatgc ttcaaaatcg acagcacctt
      301 caggtccttc aggtatatta tgtagttctt ggagcaacga accgattcgt tgtttcctct
      361 gctcttcgga aacagagctg acctcaatat catattcatc atgaatatca tcagcagcat
      421 cgggagtcat ctctgattca tgaacactta atgctagcca gtagcatgga tgcaatagca
      481 attttgaatc tggtgtaaat tctttttcag gttccttacc gtcatggcaa aatacatatg
      541 aagatgactg ctggttatac aacccaataa atccaacact gtaaagtcgt tgaatcacct
      601 ggattggccc ttcaaaaaga agtaaatcct gcattttcat cttgtcaata acgccattga
      661 gatcaagggc atattgtatc ttagatgacg cttcagaaac actgtagtaa ggtttgctat
      721 tagcaaattg cgatgtaaat atatctaatg caggaaatac attctcat
//
LOCUS       DAPGWD010000381          762 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.385, whole genome shotgun sequence.
ACCESSION   DAPGWD010000381 DAPGWD010000000
VERSION     DAPGWD010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.385"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            8..307
                     /locus_tag="RHX88_004609"
     CDS             8..307
                     /locus_tag="RHX88_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV2233189.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNLLLS"
     gene            322..>762
                     /locus_tag="RHX88_004610"
     CDS             322..>762
                     /locus_tag="RHX88_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDV2233190.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDAINYDRLVKLSLNSNALESINFP"
BASE COUNT          268 a          155 c          129 g          210 t
ORIGIN      
        1 ctgttaaatg aaattaaaaa tagttgcggt ggttgtaact ggtttgttag ctgcgaacgt
       61 agcacacgcc gccgaagtct ataacaagga tggtaataaa ctcgaccttt atggcaaggt
      121 taccgctcta cgttatttta ctgatgataa gcgtgacgat ggtgataaaa cttatgcccg
      181 tctcggcttt aaaggagaaa cgcaaatcaa tgatcaaatg attggttttg gtcactggga
      241 atatgatttt aaaggctata acgatgaagc caacggctcg cgcggcaaga atctcttatt
      301 gagttaagtg cccatcacaa tatgatcact gaccttatat tacacaatca tcccaggatg
      361 aaaacaatca ctttaaacga caaccatatt gcacatttaa acgccaaaaa cactacaaaa
      421 ctggaatatt taaacttaag caataacaat ttactgccaa ccaatgacat tgatcaacta
      481 atatcatcaa aacatctttg gcatgtatta gttaacggca tcaacaatga tccacttgca
      541 caaatgcagt actggactgc agtaagaaat ataattgatg acactaatga agtgaccatt
      601 gatttatcag gacttaattt aaccactcaa ccaccagggc tgcaaaactt cacctctatc
      661 aatcttgata ataaccaact cacacatttt gatgcaatca actacgatag actcgtaaag
      721 ctaagtctga atagtaatgc tcttgagtca ataaattttc ct
//
LOCUS       DAPGWD010000382          745 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.386, whole genome shotgun sequence.
ACCESSION   DAPGWD010000382 DAPGWD010000000
VERSION     DAPGWD010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.386"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            65..484
                     /locus_tag="RHX88_004611"
     CDS             65..484
                     /locus_tag="RHX88_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233191.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLSKQNSAP
                     GEWKTEIIEHSPSVPCGDEYIALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPEFQTSRFLDNADHYGDF"
     gene            486..>745
                     /locus_tag="RHX88_004612"
     CDS             486..>745
                     /locus_tag="RHX88_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233192.1"
                     /translation="MNKNTYDTIYSLINYYEDDYLLPLNRAELEAYKENTPAALNEAF
                     KHWDLAVNAFEHLSKRVEMLCKRENAYLTADQIWELSNWIED"
BASE COUNT          256 a          140 c          141 g          208 t
ORIGIN      
        1 ttgtttttta tgctcgtaga aacatatttc ggggtgtttt cctttaaaag aaaaggaaaa
       61 gtaaatggca aaaagaaaaa acaacgttgt taagaaaatc ggtgattcag cttctcttct
      121 aagtaaacct aaatctattc taagacgcaa agactttaaa gagctagtta cgctctccaa
      181 gcaaaatagc gcccctggcg aatggaaaac agagatcata gaacattctc cctctgtgcc
      241 ttgtggagat gaatatatcg cattacaaga aatcttatct tcaacacctg gcgtattctg
      301 gaaacctaga aaaagaaaag agtatatcgt tgatagctct gatctacgca aataccagat
      361 tttaggattt gaagattata atcactacgt tggttatctc gccaccaatg gcctaaataa
      421 tttagttcca gaattccaga cttccagatt tttagataat gctgatcact atggagactt
      481 ttaatatgaa caaaaacacg tatgacacaa tttactcact aattaattat tatgaggatg
      541 attacctcct gcctttaaac cgcgctgagc ttgaagcata caaagaaaac accccagcgg
      601 cactaaatga ggcgtttaaa cactgggatt tagctgtgaa tgcctttgag cacctctcta
      661 agcgtgtaga gatgctctgt aagcgtgaga acgcatatct aacagcggat cagatatggg
      721 agttatcaaa ctggatagaa gatat
//
LOCUS       DAPGWD010000383          742 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.387, whole genome shotgun sequence.
ACCESSION   DAPGWD010000383 DAPGWD010000000
VERSION     DAPGWD010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.387"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>742)
                     /locus_tag="RHX88_004613"
     CDS             complement(<1..>742)
                     /locus_tag="RHX88_004613"
                     /inference="COORDINATES: protein motif:HMM:TIGR04415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2233193.1"
                     /translation="DTTIEVGLQTVFDGGSVNGTIIDGGEQHISSGGSAINTTLDGYQ
                     TVFNGGNATGTTINGGFQDISSGGSATSTVINAGFQTLYDNSIASGTVINSGFQLISS
                     GGSSVDTTIQGGIQEVGEGGSASATTINDGFQFISSGGNATSTTINSGWQEISSGGSA
                     TETVINGGIQTIYDGGSASEITINSGYQVISSGGSVTTTTIYHGGEQSITNAGLATGS
                     IIRGGEQRVSSGGSAVDTTIEGGLQTVFG"
BASE COUNT          179 a          215 c          159 g          189 t
ORIGIN      
        1 ccgaaaacag tctgcagtcc cccctcaatg gtggtgtcaa cggcactgcc accgctggaa
       61 actcgctgct ccccgccacg aatgatggat ccggtcgcaa gacctgcatt ggtaatactc
      121 tgctccccac catggtaaat ggtcgtagtg gttacactgc cgccgctgga gatgacctga
      181 tagccactgt tgattgtgat ctccgaagct gagcctccat cgtaaatcgt ctggatacct
      241 ccgttaatga ccgtttcagt tgcagatcca ccactggata tttcctgcca gccactattg
      301 atagtggtac ttgtggcatt tcctccactg ctgatgaact gaaaaccatc atttatagtc
      361 gtagcacttg cacttccccc ctcaccaact tcctgaatcc caccttgaat agtggtgtca
      421 acagaactgc caccactgga aatgagctgg aagccactat taataacggt gccgcttgca
      481 atactgttat cgtagagagt ctggaatccg gcattaataa cggtgctggt agcactgcca
      541 ccactggaaa tatcctggaa cccgccatta atagtggtgc ctgttgcgtt gccaccattg
      601 aaaactgtct gatagccatc cagtgtagtg ttaatcgcac ttcccccact ggaaatgtgc
      661 tgctccccac catcaatgat cgtgccatta acacttccgc catcaaaaac agtctgtagt
      721 cccacctcaa tggtggtgtc ta
//
LOCUS       DAPGWD010000384          739 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.388, whole genome shotgun sequence.
ACCESSION   DAPGWD010000384 DAPGWD010000000
VERSION     DAPGWD010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.388"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..338
                     /locus_tag="RHX88_004614"
     CDS             <1..338
                     /locus_tag="RHX88_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2233194.1"
                     /translation="TVIKDVLLWIEHNLDQSLLLDDVANKAGYTKWYFQRLFKKVTGV
                     TLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKYIFKVTPSYYRRN
                     KLWELEAMH"
     gene            complement(348..>739)
                     /locus_tag="RHX88_004615"
     CDS             complement(348..>739)
                     /locus_tag="RHX88_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2233195.1"
                     /translation="EIRTPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSER
                     FDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYT
                     ITREDSLVAATLLTQNLFTHGYVLGKL"
BASE COUNT          203 a          143 c          170 g          223 t
ORIGIN      
        1 caacggttat caaagatgtt ctattatgga ttgaacataa tttagatcag tctttactgc
       61 ttgatgatgt ggcgaataaa gcgggttata ccaagtggta ttttcagcgg ctgttcaaaa
      121 aagtaacagg ggtcacactg gctagctata ttcgtgctcg tcgtttgacg aaagcggctg
      181 ttgagttgag gttgacgaaa aaaactatcc ttgagatcgc attaaaatat caatttgatt
      241 cccaacaatc ttttacacgt cgatttaagt acatttttaa ggttacacca agttattatc
      301 ggcgtaataa attatgggaa ttggaggcaa tgcactgaga gatcccctca taatttcccc
      361 aaaacgtaac catgtgtgaa tagattttga gtaagcaggg ttgcagccac gagtgagtct
      421 tcccttgtta ttgtgtagcc agaatgccgc aaaacttcca tgcctaagcg aactgttgag
      481 agtacgtttc gatttctgac tgtgttagcc tggaagtgct tgtcccaacc ttgtttctga
      541 gcatgaacgc ccgcaagcca acatgttagt tgaagcatca gggcgattag cagcatgata
      601 tcaaaacgct ctgagctgct cgttcggcta tggcgtaggc ctagtccgta ggcaggactt
      661 ttcaagtctc ggaaggtttc ttcaatctgc attcgcttcg aatagatatt aacaagttgt
      721 ttgggtgttc gaatttcaa
//
LOCUS       DAPGWD010000385          726 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.389, whole genome shotgun sequence.
ACCESSION   DAPGWD010000385 DAPGWD010000000
VERSION     DAPGWD010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.389"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            71..301
                     /locus_tag="RHX88_004616"
                     /pseudo
     CDS             71..301
                     /locus_tag="RHX88_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
     gene            410..>726
                     /locus_tag="RHX88_004617"
     CDS             410..>726
                     /locus_tag="RHX88_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDV2233196.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKR"
BASE COUNT          216 a          156 c          136 g          218 t
ORIGIN      
        1 tgcaccggag aaattgtaag cattactatg ccgactcttt tttatcttat tttaaaatgg
       61 aataccggac atgttaatta accgcaatat tgtggcgtta tttgcgttgc cttttatggc
      121 aagcgcaact gcttctgaat tatccattgg tgccggtgcg gcttataatg aatcgcctta
      181 tcgcggttat aatgaaaata caaaggcaat tcccctgatt agttatgaag gtgattcttt
      241 ttatgttcgt cagaccacgt taggttttat tctgtcgcaa agtgaaaaaa atgaacttag
      301 ccctgatgaa tcccctaatg attttggtaa aaatcattaa gttaaggtgg atacacatct
      361 tgtcatatga tcaaatggtt tcgcgaaaaa tcaataatca gacaacaaga tgtgcgaact
      421 cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa aacgactcaa
      481 cagcttaacg ttggcttgcc acgcattact tgactgtaaa actctcactc ttaccgaact
      541 tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa tcgaccgatt
      601 gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc atgctagctt
      661 tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata ttcgtgagca
      721 aaaacg
//
LOCUS       DAPGWD010000386          709 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.390, whole genome shotgun sequence.
ACCESSION   DAPGWD010000386 DAPGWD010000000
VERSION     DAPGWD010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.390"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            130..696
                     /gene="slp"
                     /locus_tag="RHX88_004618"
     CDS             130..696
                     /gene="slp"
                     /locus_tag="RHX88_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDV2233197.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          216 a          148 c          158 g          187 t
ORIGIN      
        1 gaactaaaca gaagggatat aaacatcaga caggtttacg ttactatcag gcatatcacc
       61 tcagaatcag atgaaaacta taaagaaata tctattatgg ttttaatatt tgttgataag
      121 gatagtaaca tgaacatgac aaaaggtgca ctcatcctca gcctttcatt tttgcttgcc
      181 gcatgtagtt caattccgca aaatatcaaa ggcaataacc aacctgatat tcaaaaaagt
      241 tttgttgctg ttcataacca gccggggtta tatgttggtc aacaagcgcg ctttggtggg
      301 aaggttatca acgttatcaa tggcaaaacg gatacgttgt tagaaatcgc tgtattaccg
      361 ttggatagct atgcgaagcc tgatattgaa gccaactatc agggccgact gctcgccaga
      421 caaagcggct tccttgatcc agtgaactat cgtaatcact ttgttaccat cctcggtacc
      481 attcagggtg aacaaccagg ctttatcaat aaagtcccgt ataacttcct ggaagtgaat
      541 atgcagggca tccaggtgtg gcatttgaga gaagtggtta ataccaccta taacctgtgg
      601 gattacggct atggtgcatt ctggccggaa ccgggctggg gtgcgcctta ctacaccaat
      661 gcggtgagtc aggtaacacc tgagctggtc aaataacacc accgaaaga
//
LOCUS       DAPGWD010000387          708 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.391, whole genome shotgun sequence.
ACCESSION   DAPGWD010000387 DAPGWD010000000
VERSION     DAPGWD010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.391"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            41..>708
                     /locus_tag="RHX88_004619"
     CDS             41..>708
                     /locus_tag="RHX88_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2233198.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDAL"
BASE COUNT          232 a          123 c          158 g          195 t
ORIGIN      
        1 ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa
       61 tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg
      121 tgtagaaatc tatgctgctg ccaaacaagg tgaacccgat ccagaattaa acacatcttt
      181 aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc
      241 aattgataaa gccaaaggcg gcggagatga aacgttcgta cagggacgtt atgaaggctt
      301 tggtcctaat ggctcaatga ttattgctga gacattgact tcaaatgtta accgtacgat
      361 tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt
      421 cagctatatg tttgacaata cgggtgtgat tgtatttaaa ggaacagacc ctgaccatat
      481 ttttgaaatt ttgcttgaag ctgaagttga tgttcgcgat gtgaccgaag aagaaggtaa
      541 tattgttatt tatactgaac ctactgacct tcataaagga atcgcggcgc taaaagcagc
      601 tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct
      661 ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttga
//
LOCUS       DAPGWD010000388          699 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.392, whole genome shotgun sequence.
ACCESSION   DAPGWD010000388 DAPGWD010000000
VERSION     DAPGWD010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.392"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..611
                     /locus_tag="RHX88_004620"
     CDS             <1..611
                     /locus_tag="RHX88_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HDV2233199.1"
                     /translation="HPLERALRALVVRHQALTDMHTQELNRTETAREVQRPSIDAHLL
                     WLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHAR
                     QFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRL
                     AANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          174 a          172 c          225 g          128 t
ORIGIN      
        1 ctcacccgct tgaacgcgcg ttgcgtgccc tggtagtccg ccaccaggcg ctgacagata
       61 tgcacacgca ggaactgaat cgcactgaaa cggcgcggga agtccagaga ccgagcattg
      121 atgctcacct tctgtggctt gaagcagagc tgaagcgtct tgagaagcag ataaaagacc
      181 tgacagacga tgatccggat atgaaacacc gcaggaaact gctggaaagc atcccgggta
      241 tcggagagaa aacatctgcg gtattgctgg cttatatcgg tctgaaggac cgcttcgccc
      301 atgccagaca gttcgccgct tttgcgggtc tgacaccacg gcgttatgaa tcaggtagca
      361 gtgtgagagg ggcgagccgg atgagtaagg ccggacatgt gtcgcttcgc agggcgttgt
      421 atatgcccgc aatggtagcc accagtaaga ctgagtgggg acgggcgttc cgcgaccgtc
      481 tggcggctaa tggcaagaaa ggaaaggtga ttctcggcgc gatgatgcgc aagctggcac
      541 aggtggcgta tggagtgctg aagtcaggcg tgccgttcga tgcgtcacgg cataatccgg
      601 tagcggcgta aaaatcgcgg aagggatgaa aaaaacagcg cctgacggcg ctgtgtctgg
      661 catgcctgca atccgggaaa ccggaccagg aaaaaactt
//
LOCUS       DAPGWD010000389          694 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.393, whole genome shotgun sequence.
ACCESSION   DAPGWD010000389 DAPGWD010000000
VERSION     DAPGWD010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.393"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>694)
                     /locus_tag="RHX88_004621"
     CDS             complement(<1..>694)
                     /locus_tag="RHX88_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2233200.1"
                     /translation="KGAGYSLTQYGIVTGGDKRFDLSGGVLSLGAFVSFSDNNIRHNR
                     GGKSSIDSKSVGLYMTYDTHGYYIDGVLKFNRFKNELRTHMSDGTMVSGDYHQNGFGG
                     SLEVGKYFSLNENTWLLPYARTTALAVNDDNISLSNGMKAEINTTHSFQGEVGVNLGM
                     DLDVGKTKIRPYLTAGVSHEFSNHNKVKINKAYDFTNDISGTTGKYGLGMDVQLTSNA
                     SIWGEANYLKGNN"
BASE COUNT          196 a          170 c          114 g          214 t
ORIGIN      
        1 cattgttacc ttttaaataa ttagcctctc cccatatact ggcatttgat gttagctgaa
       61 catccatacc caaaccgtac tttccggttg tcccggatat gtcgttagta aaatcatagg
      121 ctttattgat tttaacctta ttatgatttg aaaattcatg actgactcct gcggtcaggt
      181 agggccttat tttggtttta ccaacatcaa ggtccatccc taaattaaca ccaacctcac
      241 cctggaaaga atgtgttgtg tttatttcag ccttcattcc attagataga ctgatattgt
      301 catcattcac agcaagagct gttgttcggg cataaggaag taaccaggta ttctcattca
      361 gagaaaaata cttgcccacc tccaggctac caccgaaacc attttgatga taatctcccg
      421 aaaccatagt tccatcactc atatgggtac gcaactcatt tttgaaacgg ttgaatttga
      481 gtacaccatc tatatagtat ccgtgagtat catacgtcat gtacaagcca acacttttac
      541 tatcaataga acttttccct cctcgattat gcctgatatt gttatcagag aaggaaacaa
      601 atgcccctaa tgacaacacc ccaccgctta ggtcaaaacg cttatctccg cccgtcacta
      661 ttccgtattg ggtcagggag taacccgctc cttt
//
LOCUS       DAPGWD010000390          692 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.394, whole genome shotgun sequence.
ACCESSION   DAPGWD010000390 DAPGWD010000000
VERSION     DAPGWD010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.394"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..553)
                     /gene="fimE"
                     /locus_tag="RHX88_004622"
     CDS             complement(<1..553)
                     /gene="fimE"
                     /locus_tag="RHX88_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDV2233201.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWE"
BASE COUNT          210 a          175 c          142 g          165 t
ORIGIN      
        1 tttcccatag tccggcaaaa cgagcagcat tactggcggt ataacgtaca gtatggcgaa
       61 tatttcgatg cccgagataa tcctgaatta aacgggtatc ggcaccacgc tccgccagtt
      121 cataaccaca agcatgcctt aacatatgag gatgagtctg cgttacggtt ccagcttcaa
      181 taccggcatc gcgaataatg cgataggcct gctggcgaga aagccgactc ccgcggcgag
      241 aaataaatat agcgtcggtc cggtcagcgc ctttccagtt agcacgttcc tgggtccagc
      301 gttccacggc ttcacgctca tcaaaacgta acgggtgaac ggtagaaaat ccgttcttca
      361 gtcggcgaat atttattcga ccttcattaa ggtcaagatc ctgataatgc agatcaagca
      421 gttcactaat acgcatccca tgccgatatg ccaacagaat aagacaataa tctctggctc
      481 ccgttgcccc gtaacaaacc gcctgcatca tggcctgaac ttctttaccg gtaagataac
      541 gacgtttact cacaatagta gtactcctaa ctgagatata tttgaatgaa tacctatagg
      601 aaacctcaat cggtcaaaat tagcccaata gataaaaaga taaccacaca cgaagtgatg
      661 tggctatcag tcaattacat taaatatcat ac
//
LOCUS       DAPGWD010000391          683 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.395, whole genome shotgun sequence.
ACCESSION   DAPGWD010000391 DAPGWD010000000
VERSION     DAPGWD010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.395"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            125..>683
                     /gene="pagP"
                     /locus_tag="RHX88_004623"
     CDS             125..>683
                     /gene="pagP"
                     /locus_tag="RHX88_004623"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDV2233202.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          179 a          133 c          167 g          204 t
ORIGIN      
        1 gaacaagcgg aaataaatag agcagctatt cagattattc tttatgttgg gtctattaag
       61 gttatgttaa ttgtagcttt gctatgctag tagtagattt ttgataaatg ttttatggtc
      121 acaaatgaac gtgagtaaat atgtcgctat cttttccttt gtttttattc agttaatcag
      181 cgttggtaaa gtttttgcta acgcagatga gtggatgaca acgtttagag aaaatattgc
      241 acaaacctgg caacagcctg aacattatga tttatatatt cctgccatca cctggcatgc
      301 acgtttcgct tacgacaaag aaaaaaccga tcgctataac gagcgaccgt ggggtggcgg
      361 ttttggcctg tcgcgttggg atgaaaaagg aaactggcat ggcctgtatg ccatggcatt
      421 taaggactcg tggaacaaat gggaaccgat tgccggatac ggatgggaaa gtacctggcg
      481 accgctggcg gatgaaaatt ttcatttagg tctgggattc accgctggcg taacggcacg
      541 cgataactgg aattacatcc ctctcccggt tctactgcca ttggcctccg tgggttatgg
      601 cccagtgact tttcagatga cctacattcc gggtacctac aacaatggca atgtgtactt
      661 tgcctggatg cgctttcagt ttt
//
LOCUS       DAPGWD010000392          682 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.396, whole genome shotgun sequence.
ACCESSION   DAPGWD010000392 DAPGWD010000000
VERSION     DAPGWD010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.396"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..665
                     /locus_tag="RHX88_004624"
     CDS             <1..665
                     /locus_tag="RHX88_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233203.1"
                     /translation="VPENIPLRNAELLCSEKKLAPTVNVFTVLFNALCGNVDDINRMN
                     TLLGNLIAQRPEIITQEPEDIFYIEGDFDEELASELFRHKLIGMNIKVAALRWLRDNK
                     PGILDKSYLLSLDILAELSPWMGDDDLRLTLLKRCLVAGDAGKDALCVVLNSFADESY
                     HGLLPHDRFRKIPHSVDLWEVAELISNLGFIQPPKMGSGRDEHKIVITPVRYVRDVEF
                     YD"
BASE COUNT          180 a          130 c          171 g          201 t
ORIGIN      
        1 atgtgcctga aaacattcca ttgcgaaatg ctgaactgtt atgttcagag aaaaaactgg
       61 caccgacagt taatgtcttt acggtgttgt ttaatgctct ctgtggaaat gttgatgata
      121 ttaacaggat gaatactctg cttggtaacc ttattgccca gcgtcctgag attattaccc
      181 aagagccaga agatattttt tatatcgagg gtgactttga tgaagaactg gcaagcgaac
      241 tttttcgtca caagctaatc ggtatgaata taaaagttgc cgctttacgc tggttgcgtg
      301 ataacaaacc gggaattctt gataagagct acctgctgtc attagatatt ctggcagaac
      361 tgagtccctg gatgggtgac gatgatctgc gcctgacact gcttaaacgt tgtctggttg
      421 ccggggatgc tggcaaagac gcgctttgcg tggtgctgaa cagttttgct gatgagagct
      481 atcatggact gttaccacat gacaggttca ggaaaatccc tcactccgtg gatttgtggg
      541 aagtggccga attaatcagc aatcttggat ttattcagcc gccaaaaatg gggtcagggc
      601 gtgatgaaca caaaattgtt attactcccg tacgctatgt ccgtgatgtt gagttttatg
      661 actgagcatc attgatacgg tg
//
LOCUS       DAPGWD010000393          678 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.397, whole genome shotgun sequence.
ACCESSION   DAPGWD010000393 DAPGWD010000000
VERSION     DAPGWD010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.397"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..662)
                     /locus_tag="RHX88_004625"
     CDS             complement(<1..662)
                     /locus_tag="RHX88_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2233204.1"
                     /translation="MKRKTMGWIIVFLLFIVYMLNYMDRSALSITAPLIEKELGFNAA
                     EMGMIFSAFFVGYALFNFIGGWASDKVGPKTVFLVAALLWSVFCGMTGLVTGLWTMLA
                     VRVLFGMAEGPVSAAGNKIINNWISRKESATAIGIFSAGSPLGGAISGPVVGLLAISL
                     GWRPAFGIIFIFGLVWVLLWYFFVSDKPSKSKRLAAEERVDFENHDDIILAEDGKGTP
                     SLG"
BASE COUNT          224 a          174 c          117 g          163 t
ORIGIN      
        1 ccaagcgaag gtgtaccttt accatcttcg gctagaataa tatcgtcatg gttttcgaaa
       61 tcgacacgtt cttccgctgc cagacgttta ctttttgacg gtttatcact gacaaagaag
      121 taccacagta atacccaaac taggccaaat ataaatatga tcccaaacgc cggacgccag
      181 ccaagtgata ttgccagcaa gccaacgacc ggaccagaaa tagcgccacc aagtggtgaa
      241 ccagcactaa aaataccgat agctgttgcc gactctttac gcgagatcca gttattaata
      301 attttatttc ctgctgcact aaccgggccc tctgccatac caaataaaac acgtacggca
      361 agcattgtcc ataatccggt gaccagaccg gtcataccac aaaaaacaga ccacaacagt
      421 gcagcaacca aaaatactgt tttgggtcca actttgtcac tggcccaacc gccaatgaag
      481 ttaaataaag cataaccaac gaaaaatgcg ctgaaaatca tccccatttc agcagcatta
      541 aagcctaact ctttttcgat aagtggggcc gtaatcgata atgcagaacg atccatatag
      601 ttcagcatgt aaactataaa cagaagaaaa acgataatcc accccatggt ttttcttttc
      661 atgatatttc ctcttatt
//
LOCUS       DAPGWD010000394          664 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.398, whole genome shotgun sequence.
ACCESSION   DAPGWD010000394 DAPGWD010000000
VERSION     DAPGWD010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.398"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          189 a          148 c          142 g          185 t
ORIGIN      
        1 catcattatt aatgtttgta aagagcccac gttttcagtg ggctgattag tccaaaagct
       61 gaagttcttg gtgctgaata aatcctgaat gaattttcct tgtagtggtc aataaaaacc
      121 gaactccatt ttatttttta tttctctgca cacctcagtc agtttgatca gctgctgaaa
      181 aaactcagcg ttgaagccgc tttcaatgca gaaatggcac accatctcgg gtatgaaaaa
      241 aatcagcccc gtattaccgg gacggataac tgcaagtatc ccgcataatc gtgccattca
      301 tatttagaaa tcatccggca taatcaatct gccaacgaag gagatcgcta tgcgtacagc
      361 ccgttttact gagcatcaga tcatcgctgt gattaagtcg attgaagccg gacgaattgt
      421 taaagatgtt tgctgacctg agcctggaga accgagtgct gaaggatgtt atcgaaaaag
      481 ctttaaaaca agcctgtaag cgtgagttgg ttactcatct gataacgaca ttcgggctca
      541 gcatccgtca ggcctgccag agtttgaacc tgagcagaac ggcttaccat taccgcccag
      601 ataccacgcg tgatgaaccc gttataaacg gggctattta tatgcttacc ttttgtgtat
      661 tacc
//
LOCUS       DAPGWD010000395          657 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.399, whole genome shotgun sequence.
ACCESSION   DAPGWD010000395 DAPGWD010000000
VERSION     DAPGWD010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.399"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>657
                     /locus_tag="RHX88_004626"
     rRNA            <1..>657
                     /locus_tag="RHX88_004626"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          176 a          141 c          210 g          130 t
ORIGIN      
        1 ataatgggtc agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga
       61 aaccgagtct taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc
      121 catgggcagg ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa
      181 aattagcgga tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt
      241 ctccccgaaa gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt
      301 tcggcaaggg ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt
      361 tatcacggga gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac
      421 cgccagctaa ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag
      481 ccaggatgtt ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga
      541 gtcggcctgc gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg
      601 ctgatgcgtt gttgggtagg ggagcgttct gtaagcctgc gaaggtgtgc tgtgagg
//
LOCUS       DAPGWD010000396          653 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.400, whole genome shotgun sequence.
ACCESSION   DAPGWD010000396 DAPGWD010000000
VERSION     DAPGWD010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.400"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..544
                     /locus_tag="RHX88_004627"
     CDS             <1..544
                     /locus_tag="RHX88_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDV2233205.1"
                     /translation="KAVEEVIDSPLSEVANLIKILQSCQGKVVFIGVGKSGIIARKLA
                     ATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGETAEILATLPSLKKMGNYL
                     ISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTVVLVVGDAVALALSELKKF
                     TRADFGLYHPGGALGIKANS"
BASE COUNT          166 a          129 c          155 g          203 t
ORIGIN      
        1 taaagcagta gaagaggtta ttgattcccc cctttcggaa gtcgcgaatt taattaaaat
       61 attacaatct tgccagggaa aagttgtttt tattggtgtt gggaaatctg gcattattgc
      121 cagaaaactt gctgcaacat ttgccagcac cggaactccc tcgttttttg ttcacggtac
      181 ggaagcggta cacggcgacc ttgggatggt ggcgaaagac gatgttgtta ttcttatttc
      241 aaacagtggt gagacggcgg aaatcctggc gacactgcct agtttgaaaa aaatgggtaa
      301 ttatttgatc tcttttaccc gcagtcatca ctcatcgctt gcgataagtt gtgatttatc
      361 tgttgaaatc cccgtcaaaa gtgaggcgga taatttaggt ttagcaccgt catgctcgtc
      421 tactgttgtt ctggttgttg gtgatgcggt ggcgcttgca ctctctgaat tgaaaaaatt
      481 tactcgtgct gatttcggct tatatcatcc aggaggtgca ctcggcatta aagcaaattc
      541 ataatttcaa agtataaata cagtaaccgg tcacgccatc ttttggggtg gcgttcattg
      601 aagtgagtgt cagctatgaa aaatgttctg gtcaccgtcc ccagcttttc agc
//
LOCUS       DAPGWD010000397          651 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.401, whole genome shotgun sequence.
ACCESSION   DAPGWD010000397 DAPGWD010000000
VERSION     DAPGWD010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.401"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>651)
                     /locus_tag="RHX88_004628"
     CDS             complement(<1..>651)
                     /locus_tag="RHX88_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HDV2233206.1"
                     /translation="KKIYNAEVAEWDKLRQRLLDARNKITSAESAINSARNNVSARTN
                     EQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMVKDAIKLTSDFYRTIY
                     DEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKYNIQDRMAISKALEA
                     INQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRPFFVKLESLAAGRA"
BASE COUNT          164 a          146 c          108 g          233 t
ORIGIN      
        1 agctcttcct gctgccagtg attcaagttt tacaaaaaat ggacgccagt tgtccgtttt
       61 tacagccttt tgtaactcca cagcaacatc ataacgatca ataacttttc cggtaaaacc
      121 aaatgcctta ctgaacagct taaaattctc cgccatatgg acctgattaa tagcttccag
      181 ggctttagaa atggccatgc gatcttgtat gttatatttc ttgttcagat tattacggaa
      241 tttatcaaaa gcgttcagtg catcatccac actcttaatc tgtttccctt gagatacaga
      301 agccagctcc ttagcaagtt cggatgcttg tttaccgaac tcatcatata tcgttctgta
      361 gaaatcagag gtgagtttta tggcatcctt taccatattt atttcatccc tttttctttt
      421 ctcttcagct attttctgat tgatgtcagc aagctggcta cggatattct ctttttcctt
      481 caacagggca ttaagagcgt catttgcatg cttttgttca tttgttctgg cactgacgtt
      541 atttctcgcc gaatttatcg cagattcagc agaggtgatt ttatttctgg catcaagcaa
      601 tcgttgccgt aacttatccc attcagcaac ttcagcatta taaattttct t
//
LOCUS       DAPGWD010000398          651 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.402, whole genome shotgun sequence.
ACCESSION   DAPGWD010000398 DAPGWD010000000
VERSION     DAPGWD010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.402"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>651
                     /locus_tag="RHX88_004629"
     CDS             <1..>651
                     /locus_tag="RHX88_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HDV2233207.1"
                     /translation="QFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEII
                     FKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLL
                     RGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKT
                     VLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAH"
BASE COUNT          206 a          124 c          128 g          193 t
ORIGIN      
        1 cagttttggt cgtacgcgac atacttcagg gtattgtctt ttgaatatct gagtgaaagt
       61 atttccacac tgctgtatct ggatgccgat gttgtttgta aaggaagcct gaaacctctc
      121 acagaaatta tatttaaaga tgagtttgct gcggtcattc ctgacaatga tagtactcag
      181 gcggcatgtg caaaacgcct caacattccc gaaatgaatg gacgttattt caatgcaggc
      241 gttatctatg tcaatcttaa aaaatggcat gaagcaaatt tgacaccgta tttactcaaa
      301 cttttacgag gggaaactaa atatggctct cttaaatatt tagatcagga tgcgttgaat
      361 atcgcattta atatgaataa tatctacctt gcgaaggatt ttgatactat ttataccctg
      421 aaaaacgaac tttatgatcg tagtcatcga aagtatcagc aaaccattac cgataaaaca
      481 gtgttgattc actatacagg gataactaaa ccatggcata gctgggctgg atatccgtct
      541 gcatcatact ttaatatcgc gcgtgaacaa tctccctgga agaaatatcc tcttaaagag
      601 gcgcggactg ttgcagaaat gcagaaacaa tataagcatc tgtttgccca t
//
LOCUS       DAPGWD010000399          648 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.403, whole genome shotgun sequence.
ACCESSION   DAPGWD010000399 DAPGWD010000000
VERSION     DAPGWD010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.403"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            240..473
                     /locus_tag="RHX88_004630"
     CDS             240..473
                     /locus_tag="RHX88_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDV2233208.1"
                     /translation="MSTKNRTRRTTIRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQIRPD"
     gene            530..>648
                     /locus_tag="RHX88_004631"
     CDS             530..>648
                     /locus_tag="RHX88_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDV2233209.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLA"
BASE COUNT          233 a          152 c          111 g          152 t
ORIGIN      
        1 aaattttcat aaatagcgaa aacccgcgag gtcgccgccc cgtaacctgt cggatcgccg
       61 gaaaggaccc gcaaaatgat aataattatc atctgcatgt cacaacgtgc atctacgcca
      121 tcaaaccacg tcaaataatc aattatgacg caggtatcat attaattgat ctgcatcaac
      181 ttaacgtaaa aacaacttca gacaatacaa atcagcgaca ctgaatacgg gacaacctca
      241 tgtcaacgaa gaacagaacc cgcagaacaa caatccgcaa catccgcttt cctaaccaaa
      301 tgattgaaca aattaacatc gctcttgatc aaaaagggtc cgggaatttc tcagcctggg
      361 tcattgaagc ctgccgccgg agactgtgct cagaaaaaag agtttctcct gaagcaaaca
      421 aagaaaagag tgacattact gaattgctca gaaaacagat cagaccagat tgaagcaatt
      481 tagataatcg tgcagactac gcccctcata tcacatggaa ggtactacaa tggctcaggt
      541 tgccattttt aaacaaatat tcgataaagt gcgaaataat ttaaactatc actggtttta
      601 ttctgaacta aaacgtcaca atgtctcaca ttacatttac tatttagc
//
LOCUS       DAPGWD010000400          644 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.404, whole genome shotgun sequence.
ACCESSION   DAPGWD010000400 DAPGWD010000000
VERSION     DAPGWD010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.404"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            332..644
                     /locus_tag="RHX88_004632"
                     /pseudo
     CDS             332..644
                     /locus_tag="RHX88_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          213 a           96 c          112 g          223 t
ORIGIN      
        1 ttcaatataa tttaaaccag accccattgt gattttaact ggattaacgg atttctcttc
       61 atttttctta tttttgttct cctgatcagg aactaatggt tgcagtgata ggctattatt
      121 aatatttcgt ggcttcattt tacatttctc tcatttgatt agagcaaaat ccgaattcgc
      181 agcttctgat aatgtcaaat atcaccttat ggagacgtta cgtagaatga ctatatttgt
      241 tatcggcaca ttaggggtat taattttagt attgatggaa ggtgattatt gaacagtaac
      301 atcgagtcac ggtaaaaatg tgaggtatac aatggacatt gagttttcgc agattcatga
      361 aatggtctat atgcatgata ttgtaaattc ggactcgaaa aagaaaccaa ggattccgtt
      421 aaaaaattcc ttaatgctga gaaggttctc acgcaaacaa catcatggac attaaattca
      481 agatatgtga atgttaattc tgtcaataaa gtcaacgtaa agagtaaagt taaaaacagt
      541 tatatctcac gatctgtcaa tgatgagttt agtttaacag atgatgaaat taattctttt
      601 aaagaaacgt taatcctttc ctctattgat tcactgagta aact
//
LOCUS       DAPGWD010000401          639 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.405, whole genome shotgun sequence.
ACCESSION   DAPGWD010000401 DAPGWD010000000
VERSION     DAPGWD010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.405"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            78..>639
                     /locus_tag="RHX88_004633"
     CDS             78..>639
                     /locus_tag="RHX88_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV2233210.1"
                     /translation="MNKEMKMLNGKILVTQPFLPELREFIPYLEKIWENKWLTNNGPF
                     HQQLENDLCRYLGVEYVSLFNNATIALITAVQSLELTGEVITTPYSFVATTHSLMWNN
                     LNPVFVDVSRDTFNINPSQIEAAITEKTTAIMAVHCYGNPCDVIAIEKIAKKYKLKVI
                     YDAAHAFGVNFKGESLLKYGDLSVVSF"
BASE COUNT          230 a           87 c          118 g          204 t
ORIGIN      
        1 tggaatagga atgtatcttt tccaaaaaga tatttatgaa atggtaaagt ttttatttat
       61 aaaaacaaaa taattttatg aacaaagaga tgaaaatgtt aaatggcaag attttagtaa
      121 cgcaaccatt tttacctgaa ctaagagagt ttattcccta tctggaaaaa atatgggaaa
      181 ataaatggtt aacaaacaat ggtccatttc atcagcaatt agaaaatgat ttgtgccgtt
      241 atttaggcgt ggagtacgtc tccttattta ataatgctac aattgctctg attacggcag
      301 tccaatcatt agaattgact ggtgaagtaa ttacaacccc gtattcattt gtggcaacaa
      361 ctcactcatt gatgtggaat aatctaaatc cagtatttgt cgatgtcagt agagatacat
      421 ttaatatcaa tccgtctcaa attgaagcgg cgataacaga aaaaacaaca gccataatgg
      481 cagtccattg ttatggtaat ccttgtgatg tgattgcaat agaaaaaata gccaaaaagt
      541 ataaacttaa agtgatatat gatgctgctc atgcttttgg ggtaaatttt aagggggaaa
      601 gtttattaaa atacggagat ttatcggttg ttagttttc
//
LOCUS       DAPGWD010000402          638 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.406, whole genome shotgun sequence.
ACCESSION   DAPGWD010000402 DAPGWD010000000
VERSION     DAPGWD010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.406"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..223
                     /locus_tag="RHX88_004634"
                     /pseudo
     CDS             1..223
                     /locus_tag="RHX88_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            257..>638
                     /locus_tag="RHX88_004635"
     CDS             257..>638
                     /locus_tag="RHX88_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233211.1"
                     /translation="MGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKG
                     DGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLG
                     LFKNCQDWSNDSLETCKMKCSGNNI"
BASE COUNT          191 a          120 c          170 g          157 t
ORIGIN      
        1 gggcaacctg ctgaatgaag agaacccgca tcagctgcag cagcttatcc ggctgccggg
       61 gcagcagtat gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct
      121 gcaggggcgg tatatcactc aggatccgat tgggctgaag gggggatgga atttttatca
      181 gtatccgttg aatccgatct caaatataga tccattagga ttagaaacac taaaatgcat
      241 taagccactg cattcaatgg gcggaactgg tgaaagaagc ggtccagata tatgggggaa
      301 tccgttctat catcaatatc tttgtgtccc agatggtaaa ggggactata cttgtggtgg
      361 ccaagaccaa cggggagaat caaaaggaga tggtctatgg gggccaggta aagcaagtaa
      421 tgatacaaaa gaagctgctg gccgttgtga cctcgttgaa accgataata gttgtgtgga
      481 gaactgttta aaagggaagt ttaaagaggt aaggccgcgt tattctgtat tgcctgatat
      541 attcacacct ataaatttag ggctatttaa aaactgccaa gactggtcta atgattcttt
      601 agaaacatgt aagatgaagt gctccggaaa taacattg
//
LOCUS       DAPGWD010000403          620 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.407, whole genome shotgun sequence.
ACCESSION   DAPGWD010000403 DAPGWD010000000
VERSION     DAPGWD010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.407"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            293..580
                     /locus_tag="RHX88_004636"
     CDS             293..580
                     /locus_tag="RHX88_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233212.1"
                     /translation="MSTRNIQVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
BASE COUNT          173 a          163 c          168 g          116 t
ORIGIN      
        1 ttaagcccct gtcgccgggg gcatagacag ccacaaccgt ctccgccagt ccaggggtga
       61 aatgtacatc ccgcaaaact ttttgcccag aagcaggcaa aaacttttgc ggtctgcccc
      121 cggacagtcg tttccggccg tggcagtgat gccccatgcg gcgaaggttc tcaaaaaggt
      181 gatggtgaat accggaggga acaaaaggca ggtccccgca aaacgtcaaa accgcggctc
      241 ccgaaactca acagatgatg ggggctgaaa accagagaga ggagaaagca ggatgagtac
      301 ccgaaatatc caagttaaca ccgcgtcgta cacgcttctt gttgccggga agaaaaaaaa
      361 caccggggaa gagtgggacg tcctggaatt cagcagcctg actgagctga aaaaatatcg
      421 caaaagccac ccggaaaaga tggccttcag ttatagctac gcgctcagtc agggtgtgga
      481 taagcagttc cgccatatca acattgcgga agccgatcac ttcaaacagt tcctgcgtca
      541 gattaagcgt gccggcctcg atatccgggc gatctgctga ccggatgtga tgaatacccc
      601 tcctgattgt tgcaatcttg
//
LOCUS       DAPGWD010000404          618 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.408, whole genome shotgun sequence.
ACCESSION   DAPGWD010000404 DAPGWD010000000
VERSION     DAPGWD010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.408"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            27..581
                     /locus_tag="RHX88_004637"
     CDS             27..581
                     /locus_tag="RHX88_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV2233213.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
BASE COUNT          201 a          134 c          129 g          154 t
ORIGIN      
        1 taaaaagaaa atcgaagcat tcacacatga ataaaaaatt aatgtatata ttcgcaattt
       61 ttatagttgc agcaattacc tgtattagcc aacccaagaa aacgacgttg cgtgataaag
      121 ccatggtgaa ttatgccttt gattatttaa gatcaccggg cagtcttcca ttcaccacgg
      181 cagccacgga gctttccgcg attcatggtc actcaacgtc gcaatatcgc cttggagaat
      241 tttatcttca tggtagcgac ggtaaaccac tggattatac acaggcgaga tactggtatg
      301 agcaatcagc ggaacaggaa aatccacgcg cgcaaagtaa actggggtgg atctacctca
      361 aaggtctggg ggtcaaaccc gacacccgta aagcaattct ctggtataag gaagcagctg
      421 aacaagggta tgctcatgct caatatactt taggtttgat ctacagaaat ggctcaggta
      481 ttaatgttaa ccattatgaa tctcaaaaat ggttaaaact ggccgccaaa caacattaca
      541 aaaatgcgga aagattactt gccgggcttc ccgcacatta acgtcgactg tcaaccgaac
      601 agatggagta ttttccct
//
LOCUS       DAPGWD010000405          617 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.409, whole genome shotgun sequence.
ACCESSION   DAPGWD010000405 DAPGWD010000000
VERSION     DAPGWD010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.409"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            108..>617
                     /locus_tag="RHX88_004638"
     CDS             108..>617
                     /locus_tag="RHX88_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233214.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIIS"
BASE COUNT          233 a          106 c          124 g          154 t
ORIGIN      
        1 accaagaacc aatagaatta cctgcgaact ataaagacaa agaactcgtc cgtactatca
       61 ttaatgacaa catagttgag aagactcatg atatcaataa caaggaaatg atcttcagcg
      121 cattaaaaga aatatatgat ggagatcctg gttttatctt cgataagata tcacacaaac
      181 tcagacatac ggtaacggaa tttgatgaga gcgggaatag cgaaccaacg gacttattta
      241 cctggtacgg taaagataaa aaaggcgact ctctcgctat tgtaattaaa aataaaaacg
      301 gaaatgatta cttatctctc ggttactacg atcaggacga ctaccacatt caaagaggaa
      361 ttcgtattaa tggtgatagt ctcacccaat attgtagtga aaaagccagg aatgcttcag
      421 cgtggtttga aagcagtaaa gctatcatgg cagaatcatt tgcaacaggt tccgatcatc
      481 aggttgtaaa cgagctcaac ggggaaagac tgagagaacc taacgaagtt tttaaacgtt
      541 taggtcgagc aataagatat aattttcaag tggacgatgc aaaatttaga cgcgacaatg
      601 taaaagaaat aatttct
//
LOCUS       DAPGWD010000406          603 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.410, whole genome shotgun sequence.
ACCESSION   DAPGWD010000406 DAPGWD010000000
VERSION     DAPGWD010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.410"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            16..567
                     /locus_tag="RHX88_004639"
     CDS             16..567
                     /locus_tag="RHX88_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDV2233215.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
BASE COUNT          182 a          131 c          134 g          156 t
ORIGIN      
        1 atataaagga atattatgtc tgcttttaaa aagtcacttc ttgttgctgg cgtagcaatg
       61 attctttcca ataacgtatt tgctgacgaa ggtcacggca ttgttaaatt taaaggtgaa
      121 gttatctctg ctccatgttc aattaaacca ggtgacgaag acttaactgt aaatctgggt
      181 gaagtagcag ataccgttct gaaaagcgac cagaaatctt tggctgagcc tttcactatt
      241 cacctgcaag actgcatgtt gagccagggt ggaaccactt attcaaaagc gaaggttact
      301 ttcaccacag cgaatactat gacgggtcag actgacctgc tgaaaaacac caaagaaact
      361 gaaatcggtg gtgccacagg cgttggcgta cgtattctgg atagccagag cggcgaagtg
      421 actttaggta ctccggtagt aattacattc aacaatacta acagctatca ggaactgaac
      481 ttcaaagctc gtatggaatc cccaagcaaa gatgctaccc cgggcaacgt ttacgctcag
      541 gctgactata agattgctta cgagtaatca cattaggccg ggttcgcccg gccacatcat
      601 taa
//
LOCUS       DAPGWD010000407          593 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.411, whole genome shotgun sequence.
ACCESSION   DAPGWD010000407 DAPGWD010000000
VERSION     DAPGWD010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.411"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..314
                     /locus_tag="RHX88_004640"
     CDS             <1..314
                     /locus_tag="RHX88_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001414580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HDV2233216.1"
                     /translation="GNIAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYN
                     RNRYYDPLQGRYITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALMRKKEQLNH
                     Q"
BASE COUNT          179 a           97 c          170 g          147 t
ORIGIN      
        1 atggaaacat tgcgtggagc ggggagtatg atgagtgggg caaccagctg aatgaagaga
       61 acccgcatca cctgcaccag ccgtaccggc tgccggggca gcagtatgat aaggagtcgg
      121 ggctgtacta caaccggaac cggtactacg atccgttgca ggggcggtat atcactcagg
      181 acccgatagg gctggagggg ggatggagtc tgtatgcgta tccgctgaat ccggtgaatg
      241 gtattgatcc attagggtta agtcctgcag atgtagcgtt aatgcgaaaa aaagaacaac
      301 taaaccatca atgagcatgg gatatattat ctgatactta tgatgatatg aagagattaa
      361 atttaggtgg gactgatcaa tttttccatt gtatggcatt ttgtcgagtg tctaaattaa
      421 atgacgctgg tgttagccga tcagcgaaag ggctgggtta tgaaaaagag attagagatt
      481 acgggttaaa tatgttcggt atgtacggca gaaaagtaaa gttatcccat tctgagatga
      541 ttgaagataa taaaaaagac ttggctgtaa atgaacatgg gttgacatgt cca
//
LOCUS       DAPGWD010000408          593 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.412, whole genome shotgun sequence.
ACCESSION   DAPGWD010000408 DAPGWD010000000
VERSION     DAPGWD010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.412"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            79..>593
                     /locus_tag="RHX88_004641"
     CDS             79..>593
                     /locus_tag="RHX88_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2233217.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSAIKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAV"
BASE COUNT          172 a          115 c          140 g          166 t
ORIGIN      
        1 cggtcaatta ttgttattat taagtctaat gtcatttaaa ggcattatca tatttacgaa
       61 gagatcagga tgatgacgat gaaaaaaagt gtattgacgg cgtttataac tgtggtatgt
      121 gcaacgtcca gcgttatggc agctgatgat aatgctatca cggatggcaa agtaacattt
      181 aatggtaaag ttattgctcc agcttgtacc ctggtagctg cgacgaaaga ttcagtagtc
      241 acattgccaa atgtcagcgc catcaagttg caaactaatg gagcagtctc tggcgttaaa
      301 actgatgtac caattgcctt agaaggctgt gatgttactg taactaaaaa tgcgacgttt
      361 acctttagtg gtacggctga cggcgtgcaa cctacagcat ttgccaacca ggctaccacc
      421 gatgcagcga cgaatgttgc tctacaaatg tatctgccag atggttctac atcggtaact
      481 cccggtaccg aaacgtcgaa tattcagtta gcggattctg ctgagcaaac agtgaccttt
      541 aaagttgatt atatcgctac ggggaaagcg acttctggta atgtgaatgc ggt
//
LOCUS       DAPGWD010000409          581 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.413, whole genome shotgun sequence.
ACCESSION   DAPGWD010000409 DAPGWD010000000
VERSION     DAPGWD010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.413"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..475
                     /locus_tag="RHX88_004642"
     CDS             <1..475
                     /locus_tag="RHX88_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV2233218.1"
                     /translation="FILGEFAGGMMGSELVKWVKSHKIDAQIITFVAKMPYIDSIKLL
                     EAGAKGCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEI
                     LQLIADGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          196 a           86 c          116 g          183 t
ORIGIN      
        1 atttatttta ggagaatttg caggtggaat gatggggtcc gaactcgtaa aatgggttaa
       61 atcgcataag atagatgctc aaattattac atttgtagca aagatgccgt atattgattc
      121 aataaaattg cttgaagcag gtgcgaaagg atgcgtatgg aaaaccagtc acccggcgaa
      181 actaaatcgt gctattgatt cgattagtaa tggttacact tattttgata gtgtacatat
      241 ggattgtgaa aaaatatctt ccaggtattc ctctgataat caacttacaa atcgtgagtc
      301 agaaatatta caactcatag ctgatggtaa aacaaacaaa gaaatcgcta atttcttgca
      361 gttaagcaga aaaacagtcg aaactcatag actcaatatc atgaagaaat tagatgttca
      421 cagtggtatc gagttgatca aaacagcttt acgtatgggt gtttgcacta tataaaaaaa
      481 agaggcttta ggctcatttt gctgaagcct tacgattgtc tagttcatta agcctggtat
      541 atacaatata tattttgggg catattgctg tctctttttt t
//
LOCUS       DAPGWD010000410          580 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.414, whole genome shotgun sequence.
ACCESSION   DAPGWD010000410 DAPGWD010000000
VERSION     DAPGWD010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.414"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..460)
                     /locus_tag="RHX88_004643"
     CDS             complement(<1..460)
                     /locus_tag="RHX88_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233219.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTD"
BASE COUNT          163 a          126 c           89 g          202 t
ORIGIN      
        1 catcagtttt atcaagctta gttttcagcc ttttaacagc ctccaaggaa gcatcaagaa
       61 ctggtgtctg attcttcaaa gcttctgaaa aacttttttg tgttgatttt cgagcttgtt
      121 gttgagcaga ttttcttaac tctttttcac gcttaacttg ttctaaaagc tctttacggt
      181 catcagaacg tgaaccaaaa aatctatcag caagttcagt taacaaatta gatataatag
      241 ttttcagttc tcttgcagcc tggtttctta tgaatccctc tctccccgac ttatctttca
      301 gctctttatt actggattga gtaattccaa tataaccaaa aatccttcta ttagaccaat
      361 aatatcgccc tgcattccat gaacgtcttt cttctatctg gaagaaatca ttatctactc
      421 gaccataagg taatactctc aagctatccc taaaaatcat tagtcctgca tactttttgg
      481 ccttaagatc aaagtgggaa tgttcacgtt cagtatgtga tgtattttgt gaaaggaatt
      541 caaacgttcc tatctgaagc tcaaatgggc cgacccctgc
//
LOCUS       DAPGWD010000411          571 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.415, whole genome shotgun sequence.
ACCESSION   DAPGWD010000411 DAPGWD010000000
VERSION     DAPGWD010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.415"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>571
                     /locus_tag="RHX88_004644"
     CDS             <1..>571
                     /locus_tag="RHX88_004644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233220.1"
                     /translation="NIGANNNFIETFKTSVGQWLAICEGDDYWCDQGKLQKQYDYAIS
                     HSDCSLVVHPALVSENNVIRKTSWACMNKTINQLSDVIRAKGQFSPTGSYFFKREILN
                     VLPLWFSTAPVGDYYMEIFATSLGSCHTIPDAMSVYRINSTGSWSDLLKKDRNGQRII
                     NTYLSQLEYLDKLAEIFPSCVDDITIKRSH"
BASE COUNT          184 a           96 c          109 g          182 t
ORIGIN      
        1 aatattggag caaataataa ttttatcgaa acatttaaga cttcagtagg tcaatggcta
       61 gctatatgtg agggagatga ttattggtgt gatcagggga aattacaaaa acagtacgac
      121 tacgctattt cacatagtga ttgctcatta gtcgtgcatc ccgctctggt aagtgaaaat
      181 aatgtaatac gtaaaacatc ttgggcgtgt atgaataaaa caataaatca gcttagcgat
      241 gtaatacgtg caaaaggaca gttctcacca actggctcct attttttcaa acgagaaatc
      301 ttaaatgttc taccattgtg gttttcaaca gctcctgttg gtgactatta catggaaatc
      361 tttgctacat cgcttggatc ttgtcataca attcctgatg ctatgtcagt ttatagaatc
      421 aattcaacgg ggtcttggtc tgacctgtta aaaaaagata gaaatggcca acggattatt
      481 aatacttatc tttctcaatt ggagtatttg gataaacttg ccgaaatctt tccttcttgt
      541 gtcgatgata tcacaataaa aagatcgcat a
//
LOCUS       DAPGWD010000412          562 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.416, whole genome shotgun sequence.
ACCESSION   DAPGWD010000412 DAPGWD010000000
VERSION     DAPGWD010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.416"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..378)
                     /locus_tag="RHX88_004645"
     CDS             complement(<1..378)
                     /locus_tag="RHX88_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV2233221.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIF"
     gene            complement(387..554)
                     /locus_tag="RHX88_004646"
     CDS             complement(387..554)
                     /locus_tag="RHX88_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein"
                     /protein_id="HDV2233222.1"
                     /translation="MLARYASQLNAFAISLTVKSALAFLILIIYFAPILAERVMPLSF
                     FPEQLQLYIEK"
BASE COUNT          189 a          101 c           99 g          173 t
ORIGIN      
        1 aaatatacgc tttaagccat ttactggatt caatgaatca aacttaaatt taacagcttc
       61 agttgctaat tgcattttac tttgaaacca ggagacaagt attgttgtga acatgcaaac
      121 cagcaaaaaa ggaatgttcg ttttcaacca accaaccccc atagctttaa aatagtccca
      181 aatatctggt ttagcgttat gatcaaggat gtattcaaga atcgacataa tgtgatgcac
      241 atcaaaaaca tatccgagat ataaagtacc cacaagcatt attacagaga tggttaagtc
      301 tctacttttt agaatttgcc cttttttaga agcatcctgg agtttctttt gtgttggctt
      361 ttcagtttta ttggccatga aacactctat ttctcaatgt atagttgcaa ttgttctggg
      421 aaaaaggata acggcattac tcgttcagcc agtattgggg caaaataaat aattaatatt
      481 aaaaatgcca gcgcactttt gacagtcagt gaaatggcaa aagcatttag ttgtgaagca
      541 taacgtgcca gcaatcccag ga
//
LOCUS       DAPGWD010000413          558 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.417, whole genome shotgun sequence.
ACCESSION   DAPGWD010000413 DAPGWD010000000
VERSION     DAPGWD010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.417"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(33..108)
                     /locus_tag="RHX88_004647"
     tRNA            complement(33..108)
                     /locus_tag="RHX88_004647"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Lys,seq:ttt)
     gene            complement(255..330)
                     /locus_tag="RHX88_004648"
     tRNA            complement(255..330)
                     /locus_tag="RHX88_004648"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(295..297),aa:Lys,seq:ttt)
     gene            complement(334..409)
                     /locus_tag="RHX88_004649"
     tRNA            complement(334..409)
                     /locus_tag="RHX88_004649"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(374..376),aa:Val,seq:tac)
BASE COUNT          128 a          146 c          139 g          145 t
ORIGIN      
        1 ttgcgggatt atgctgcttt aatcattcag gatggtgggt cgtgcaggat tcgaacctgc
       61 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccat acgggtgtta
      121 cctgaaagat ttctactcgg ggccaatctt taagattggt gtgtcgtgca ggatgactcg
      181 cttcgcttgc cctacgggcc gttgctggcg caacgttatc cttcacgctt aacatctgag
      241 tttgatgtta aaattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
      301 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
      361 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
      421 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggatga
      481 ctcgcttcgc tcgccctacg ggccgttgct gacgcaacgt tatcctacac gcttaacatc
      541 ttgtcttcga tgctaaaa
//
LOCUS       DAPGWD010000414          536 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.418, whole genome shotgun sequence.
ACCESSION   DAPGWD010000414 DAPGWD010000000
VERSION     DAPGWD010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.418"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>536
                     /locus_tag="RHX88_004650"
     CDS             <1..>536
                     /locus_tag="RHX88_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2233223.1"
                     /translation="ISLTTELLLWRSSWPTLATAVRQFSQGIVSNEIETALNSSIASE
                     KVSDALGVILCVDQSDEQLSQEQRKSRKKALADAFPAVNFEREQSDLPEFCELWPIHR
                     DLQQTRLKNTVLPSGLLFVAHKYDPTTPWINARKMADKFSAPLLTINGDGHTLALAGS
                     NLCVDEAVVRHLLFPGKS"
BASE COUNT          146 a          117 c          121 g          152 t
ORIGIN      
        1 catatcatta acgacagagc ttctgttatg gcgctcatca tggccaacac ttgcaacggc
       61 cgtacgtcag ttctctcagg gtattgtcag caatgaaatt gaaactgcac ttaattcttc
      121 catagcatcg gaaaaagtca gtgatgctct gggtgtaata ttatgtgttg atcagagtga
      181 tgaacaatta tcacaggaac aacgaaaatc gcgaaaaaaa gcccttgctg acgcatttcc
      241 ggccgttaat tttgagaggg aacagtccga tttgcctgaa ttttgtgaat tatggccaat
      301 tcatcgcgat ctgcaacaga cacgcctgaa gaatactgtt ttaccatccg gtttactgtt
      361 tgtggcacac aaatatgatc cgacaactcc ctggataaat gcccgtaaaa tggcagataa
      421 attttcagct ccgttactga ctattaacgg tgacgggcat acgctggcgc tagctggctc
      481 taatttatgt gtggatgagg ctgttgttcg tcacctcctg tttccgggga aatctg
//
LOCUS       DAPGWD010000415          529 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.419, whole genome shotgun sequence.
ACCESSION   DAPGWD010000415 DAPGWD010000000
VERSION     DAPGWD010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.419"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          170 a          100 c          101 g          158 t
ORIGIN      
        1 ctgtgagata aacaattcag gtgacgtcac ctgcagcagc attgcttcat aagagttctt
       61 cggcaccaga aataccccta tattggtatg cgcatatagc tcttaaaatt gcctgaggat
      121 atatttgaca ttaacctgaa aaactgatta cagagtcaat cttaatgctg gaagattcga
      181 tctcatgctc agcaacccat ttaacaccct ttattatcaa ttaattaggc agtagcacat
      241 tacatttcct ctgctaaaag gagaacattt atctttgcag cgcagttagc ttattacgca
      301 gcaagtgcac aataagctaa cgagactgtg gattattgtt caataatgca cagaatttag
      361 acattagcac acatgcgtcc aggcagtttt agtggtgtgt ccattatggg tataagaata
      421 agatatcctt tcaggaactg ttttaaaaca aaagtgcgta tgtatttcag taatggtaat
      481 ttttatgtga caggaggctt aaccgagcgc tcttatctca agagaaaaa
//
LOCUS       DAPGWD010000416          527 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.420, whole genome shotgun sequence.
ACCESSION   DAPGWD010000416 DAPGWD010000000
VERSION     DAPGWD010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.420"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>527
                     /gene="tuf"
                     /locus_tag="RHX88_004651"
     CDS             <1..>527
                     /gene="tuf"
                     /locus_tag="RHX88_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV2233224.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGR"
BASE COUNT          128 a          130 c          146 g          123 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acattctgtc caaagatgaa ggcggccgtc atactccgtt cttcaaaggc
      361 taccgtccgc agttctactt ccgtactact gacgtgactg gtaccatcga actgccggaa
      421 ggcgtagaga tggtaatgcc gggcgacaac atcaaaatgg ttgttaccct gatccacccg
      481 atcgcgatgg acgacggtct gcgtttcgca atccgtgaag gcggccg
//
LOCUS       DAPGWD010000417          522 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.421, whole genome shotgun sequence.
ACCESSION   DAPGWD010000417 DAPGWD010000000
VERSION     DAPGWD010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.421"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            37..213
                     /locus_tag="RHX88_004652"
     CDS             37..213
                     /locus_tag="RHX88_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HDV2233225.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWGFGYPGAIIFMILA
                     GLTSYLYFKRKNWL"
BASE COUNT          155 a          102 c          102 g          163 t
ORIGIN      
        1 cgttaatgat tggtcttgct cattgttaat aacaaaatgt tgaatttacc gattaggaaa
       61 ctttttccta cgcggattgc ctccagctat ggcatgaact ttgagtttat gccagaactg
      121 aagtggggct tcggctaccc tggcgcgatt atctttatga tcctcgcggg cctgacatcg
      181 tatctgtact ttaagcgtaa gaactggctg taactatacg atgtcgctgt gagtaagtta
      241 gtgtgctgac agcgacattt tagccgaggg aatcgcagat aacataattg ttgtgaataa
      301 tatccatgcc acgttgagtt ggacgcttaa tgactcacat ctcacccctt caattgctgc
      361 agagataata gaaaaaagtg tcaaacccaa tgaaatcaat acaaagtaga ttgccttaca
      421 tccagtatct cttattcttt ttgctccaat attgatattt acataggtat tattaataac
      481 cagaataaaa ctcaaagcca ggttgatatc attcaaagtt at
//
LOCUS       DAPGWD010000418          506 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.422, whole genome shotgun sequence.
ACCESSION   DAPGWD010000418 DAPGWD010000000
VERSION     DAPGWD010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.422"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..505
                     /locus_tag="RHX88_004653"
     CDS             <1..505
                     /locus_tag="RHX88_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HDV2233226.1"
                     /translation="DFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMS
                     PRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGC
                     CYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQ
                     FENKNLA"
BASE COUNT          122 a          114 c          154 g          116 t
ORIGIN      
        1 ggatttttac gccagtggcc cgaaccagaa gtgggcagga gacatcacgt acttacgtac
       61 agatgaaggc tggctgtatc tggcagtggt cattgacctg tggtcacgtg ccgttattgg
      121 ctggtcaatg tcgccacgca tgacggcgca actggcctgc gatgccctgc agatggcgct
      181 gtggcggcgt aagaggcccc ggaacgttat cgttcacacg gaccgtggag gccagtactg
      241 ttcagcagat tatcaggcgc aactgaagcg gcataatctg cgtggaagta tgagcgcaaa
      301 aggttgctgc tacgataatg cctgcgtgga aagcttcttt cattcgctga aagtggaatg
      361 tatccatgga gaacacttta tcagccggga aataatgcgg gcaacggtgt ttaattatat
      421 cgaatgtgat tacaatcggt ggcggcggca cagttggtgt ggcggcctca gtccggaaca
      481 atttgaaaac aagaacctcg cttagg
//
LOCUS       DAPGWD010000419          505 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.423, whole genome shotgun sequence.
ACCESSION   DAPGWD010000419 DAPGWD010000000
VERSION     DAPGWD010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.423"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            197..>505
                     /locus_tag="RHX88_004654"
     CDS             197..>505
                     /locus_tag="RHX88_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HDV2233227.1"
                     /translation="MAKIQVYVNDHVSEKINAIAVQRRAEGAKEKDVSYSSIASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSTVAKILGIESLSPHVSGKPKFEY
                     A"
BASE COUNT          156 a           66 c          121 g          162 t
ORIGIN      
        1 cagtaatgac tatttttttt gaaaacatct atttacttgg cgcgtgaatc tatattagat
       61 tcgtcatttg atgattgctg attcgtatag tgaatcgtga ttttagtatg atgaatctaa
      121 tggcgattcg tcataaggtg cagaatagag agcacgaaaa tatattgttt gtttaggaag
      181 gtgaataagg tgtgttatgg ccaaaattca ggtttatgtt aatgaccatg tgtctgaaaa
      241 aataaatgct attgctgtgc aaaggcgagc agagggagca aaagagaaag atgttagtta
      301 ttcaagcatt gcctctatgt tgcttgagtt aggattgcga gtttatgaag cgcagatgga
      361 acgaaaagaa tcagcgttta atcaggcaga gtttaataaa cttcttcttg agtgtgttgt
      421 aaaaacgcag tcaacggtgg caaagatttt aggtattgag tctcttagcc ctcatgtttc
      481 cggaaaaccg aagtttgaat atgcc
//
LOCUS       DAPGWD010000420          497 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.424, whole genome shotgun sequence.
ACCESSION   DAPGWD010000420 DAPGWD010000000
VERSION     DAPGWD010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.424"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>497)
                     /locus_tag="RHX88_004655"
     CDS             complement(<1..>497)
                     /locus_tag="RHX88_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HDV2233228.1"
                     /translation="KIECACNFLMDKDAQGYIDLSDLDLTSCHFKGDVISKVSFLSSN
                     LQHVTFECKEIGDCNFTTAIVDNVIFRCRRLHNVIFIKASGECVDFSKNILDTVDFSQ
                     SQLTQSNFREYQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNMTAVMFDHVRIST
                     GNFKD"
BASE COUNT          171 a           95 c           74 g          157 t
ORIGIN      
        1 aatctttaaa attccctgtc gatattcgca catgatcaaa cataacagct gtcatattcg
       61 atttaataaa tgatatttct ttggcagaca gaaactctgc tctggtgaag tgcgaagcgt
      121 aaagataaca attatcgaag tttgaatttc taatctgata ttcgcgaaaa ttactctgag
      181 taagttgact ctgcgagaag tcaactgtat caagaatatt tttgctgaaa tcgacacatt
      241 caccactcgc tttgataaaa atcacattgt gtaaacgtcg acatctaaat atgacattat
      301 caactattgc agtagtaaaa ttgcaatccc caatttcttt acattcgaat gttacatgtt
      361 gtagatttga tgataaaaaa gacaccttcg atataacgtc acctttaaaa tgacaacttg
      421 ttaaatccaa atcagacagg tcgatatacc cctgcgcatc tttatccatc agaaaattgc
      481 aagcgcattc aattttt
//
LOCUS       DAPGWD010000421          491 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.426, whole genome shotgun sequence.
ACCESSION   DAPGWD010000421 DAPGWD010000000
VERSION     DAPGWD010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.426"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            100..>491
                     /locus_tag="RHX88_004656"
     CDS             100..>491
                     /locus_tag="RHX88_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233229.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIE"
BASE COUNT          159 a           71 c           98 g          163 t
ORIGIN      
        1 tataattttt ttattctgtg tcatcataat ttggtcatga aatttccgtc ttgttacact
       61 actctaaact agagcttatg gaattagcgg gtggtattca tggaaaataa tgataaattc
      121 ttatcacaag acttattgga atcttatgcc attcgtttgt tgagcggacc attgaatgga
      181 tgcgagtatg aaatacttaa tgggcgtcta cttgttatca ttggtaatga tgtttcgtta
      241 ggaagaagtg atgctttttc tgagttacca gaaaacacaa tagtcgttcc ttatggcgag
      301 cttacaggta gttttgagat aataattact accgaccctg atctggtagt aacattcaga
      361 gaattaacag cacaagaacc tgaagataga acgttaacat tgaatcagca aatagaagtt
      421 ttaggtctca aatttgctgt aaaagaaaaa aatgaagttt ggcagtattc attgccaggc
      481 attattgaaa a
//
LOCUS       DAPGWD010000422          485 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.427, whole genome shotgun sequence.
ACCESSION   DAPGWD010000422 DAPGWD010000000
VERSION     DAPGWD010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.427"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>485
                     /locus_tag="RHX88_004657"
     rRNA            <1..>485
                     /locus_tag="RHX88_004657"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAPGWD010000423          476 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.428, whole genome shotgun sequence.
ACCESSION   DAPGWD010000423 DAPGWD010000000
VERSION     DAPGWD010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.428"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>476
                     /locus_tag="RHX88_004658"
     rRNA            <1..>476
                     /locus_tag="RHX88_004658"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAPGWD010000424          453 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.429, whole genome shotgun sequence.
ACCESSION   DAPGWD010000424 DAPGWD010000000
VERSION     DAPGWD010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.429"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>453)
                     /locus_tag="RHX88_004659"
     rRNA            complement(<1..>453)
                     /locus_tag="RHX88_004659"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           83 a          145 c           98 g          127 t
ORIGIN      
        1 ccttctcccg aagttacggc accattttgc ctagttcctt cacccgagtt ctctcaagcg
       61 ccttggtatt ctctacctga ccacctgtgt cggtttgggg tacgatttga tgttacctga
      121 tgcttagagg cttttcctgg aagcagggca tttgttgctt cagcaccgta gtgcctcgtc
      181 atcacgcctc agccttgatt ttccggattt gcctggaaaa ccagcctaca cgcttaaacc
      241 gggacaaccg tcgcccggcc aacatagcct tctccgtccc cccttcgcag taacaccaag
      301 tacaggaata ttaacctgtt tcccatcgac tacgcctttc ggcctcgcct taggggtcga
      361 ctcaccctgc cccgattaac gttggacagg aacccttggt cttccggcga gcgggctttt
      421 cacccgcttt atcgttactt atgtcagcat tcg
//
LOCUS       DAPGWD010000425          446 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.denovo.430, whole genome shotgun sequence.
ACCESSION   DAPGWD010000425 DAPGWD010000000
VERSION     DAPGWD010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.denovo.430"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            159..>446
                     /locus_tag="RHX88_004660"
     CDS             159..>446
                     /locus_tag="RHX88_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2233230.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMR"
BASE COUNT          158 a           65 c           92 g          131 t
ORIGIN      
        1 agggactttc tctgctatga gataagaaat ttcatattat gctttaaggc tgaatgattt
       61 gtgcaaaata taattatttt tccatcgaac atgaggtgta tgtcataaga ttaaatgtta
      121 actatgcatt cctcgcggtg aattatatga ggtaaaagat gaaaattgga actgtggcag
      181 gcactaacga cagtacaacg acaatcgcaa caaatgatat ggtgcaagag catgttacta
      241 acttcactaa agaactgttt gggtatattg caaatggtat aggtgatgat atatcaagta
      301 ttgccaggac aatgctcggt gaagttgtgg aaaaaattga tgattggcaa atagaacgtt
      361 tccaacaatc tattcgagat gacaaaattt catttaccat acaaacgaat cattcagaaa
      421 aatatagtat gctatcgggc atgcgc
//
LOCUS       DAPGWD010000426        17560 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.guided.084, whole genome shotgun sequence.
ACCESSION   DAPGWD010000426 DAPGWD010000000
VERSION     DAPGWD010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.guided.084"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="RHX88_004661"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="RHX88_004661"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV2233231.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="RHX88_004662"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="RHX88_004662"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV2233232.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="RHX88_004663"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="RHX88_004663"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV2233233.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRGEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="RHX88_004664"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="RHX88_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV2233234.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="RHX88_004665"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="RHX88_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV2233235.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="RHX88_004666"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="RHX88_004666"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV2233236.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIDALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="RHX88_004667"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="RHX88_004667"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV2233237.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="RHX88_004668"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="RHX88_004668"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV2233238.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="RHX88_004669"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="RHX88_004669"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV2233239.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="RHX88_004670"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="RHX88_004670"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV2233240.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            12901..14034
                     /locus_tag="RHX88_004671"
     CDS             12901..14034
                     /locus_tag="RHX88_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV2233241.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTTGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNHRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14123..14656
                     /gene="blc"
                     /locus_tag="RHX88_004672"
     CDS             14123..14656
                     /gene="blc"
                     /locus_tag="RHX88_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV2233242.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="RHX88_004673"
     CDS             complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="RHX88_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV2233243.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15152..15298)
                     /gene="ecnB"
                     /locus_tag="RHX88_004674"
     CDS             complement(15152..15298)
                     /gene="ecnB"
                     /locus_tag="RHX88_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV2233244.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15409..15534)
                     /gene="ecnA"
                     /locus_tag="RHX88_004675"
     CDS             complement(15409..15534)
                     /gene="ecnA"
                     /locus_tag="RHX88_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV2233245.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15586..16152)
                     /gene="efp"
                     /locus_tag="RHX88_004676"
     CDS             complement(15586..16152)
                     /gene="efp"
                     /locus_tag="RHX88_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV2233246.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16194..17222
                     /gene="epmB"
                     /locus_tag="RHX88_004677"
     CDS             16194..17222
                     /gene="epmB"
                     /locus_tag="RHX88_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV2233247.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4371 a         4599 c         4519 g         4071 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgaccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgta
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtaaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaatgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtggcga agtacgccca atcgataccg
     2281 atccgaatgt actggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtac
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccg gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atctcacaag
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccgga agtcgtagag gcgctccagt
     3181 ccgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattacccg aaactttccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgaacc gcgcagcgtg tcgccgggga tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttactgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtt gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgtc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaaca
     3721 gccaacgtca gcttgaagcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 ctttgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa tctgctcggc gaagcgctgc gggcgcaggt ggcacggctg ccagaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tattggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacaattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtt ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgttaccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc actgtatctc aacaaagagg gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcgccactgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc acaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccaacgccgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcgcgt ggcgaagagg
     5281 aagcacatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccaattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtacgggtt
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 caatgattgg tattgagtgg tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc
     5761 tcggttttgg tttacaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttactggt agcgtgacga
     5881 aaattaatac ccgcgcaacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaagggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggcaggcttc catgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctgaat ggtaaacaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccagct tcaaccacag gttgaatgat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag tacgacaacc accgtcgcga tcattgccga catacgcgtt
     6721 ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagaa tgaagatagt aaccaacccg
     6901 cactgcatcc acatggcgat agacggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaatg ctttcggcgt cccctggatg atggctttca acggtgaata gcaaagcgta
     7021 aagaacgcac cggtataggc gaggaacatc gaaagtccag taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcat tacccagcgt catcccaagg
     7141 ctcttcatca gcacataggt aatattgccg aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacgcc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatgg caaataccac aaacgatagc
     7381 attgccagac cggactgata acccgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccgcc caccgcagta atgcgggcaa ttttattaat ccccttagaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtactcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagatacca
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaaacc aaatacggaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatttttt ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataacac
     8041 gtgcccactg aactgcactg cgcaacattg agcaggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttagcgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttattacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg
     8281 ccaacatcac cagacgatca acacctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcgtc aatcagattc tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttacc aatatttggc tctacgccaa aggtaaacag caattgtagc aacgtgtcgc
     8641 ggtcttcttc ggtatcagca acattgctca aatccagttt cgctgccact tcccgcagtt
     8701 gcgttttgtc ggcagagagt gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactccag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaagc tgcggcacag ctggaatacc ggcccacaac
     8941 cggcaaccag cagacgtttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaatca aacagcggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggtactta
     9481 ccctgaagta cgtggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccgaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtggggat gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgcac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggtctgcca ggttccggta tcctgatgac
    10261 cgaaggctgc cgcggtgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagttt ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgccg cgtggcgatg tggtttatct cgacttgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 tctgcacggt gcaaaccgtc tgggttctaa ctccctggcg gaactggtgg tcttcggccg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggcgaaga tccgcgacga aatgggcctg gcaatggaag aaggttgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggcag agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ctccagcgtg ttcaacaccg acctgctcta
    11041 caccattgaa ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcaccg agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctgcc gccagctaaa cgcgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagttacc gctggtcctg ccgtatggcg atttgtggtt
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggca aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctattgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttatcg tgatcattaa cctgatcact
    12301 ctggcggcag ctctgctgca taccaaaacc tggtttgaac tggcaccgaa agcagccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtggttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc
    12601 actggggctg tttccgggtg atgcgctgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aattcacgtg cctgcgggca aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtatcgtta caatctaacg
    12841 tatcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagacctt
    12901 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atccccggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccacaacaaa atactggcct gaacttaccg ctaaacagtg gaatgggatc
    13261 acactattac atctcgcaac ctacactact ggcggcctgc cattgcaggt gccggatgag
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggcgcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg caaacacgtg tcttccagcc actcaaactc
    13501 aaccatacgt ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc
    13561 gaaggcaagg cagttcatgt ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag
    13621 tcgaccattg aagatatggc ctgctgggta cgaagcaata tgaatcaccg tgatatcaac
    13681 gacaaaacac ttcaacaagg gatacaactg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gtctgggctg ggaaatgctg gactggccgg taaatcctga catcatcgtt
    13801 aacggcagtg acaataaaat tgcactggca gcacgccccg taaaagcgat tacgccccca
    13861 actcctgcag tacgcgcatc atgggtgcat aaaacggggg cgaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctaca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctgc gcttttcctt gctgtcatct acacttagaa
    14101 aaaaaccagt aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc
    14161 atttctggtc gttgcctgca gttctcctac gccgccgcgt ggcgtaacag tagtgaataa
    14221 tttcgacgcc aaacgttatc tcggtacctg gtatgagatt gcccgttttg atcaccgttt
    14281 tgaacgtgga ctggaaaaag tcaccgcaac atacagcctg cgtgatgacg gcggcctgaa
    14341 tgtcattaat aaaggctata accctgacag aggaatgtgg cagcagagtg aagggaaagc
    14401 gtactttacc ggcgcaccaa ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta
    14461 tggcggttat aacgttattg cactcgatcg ggaataccgc catgcgctgg tttgcggtcc
    14521 ggaccgcgac tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca
    14581 ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc agtaagttta tttgggtaca
    14641 gcagcccggt agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc
    14701 cagactcgcc aggcgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatggc
    14761 cgcgccgact gcgccaatac ccgtccacac ggcataagcc gtccctactg gtaacgattt
    14821 catcgcccag gcaagtagcg ccatactgac aatcatcgcc gtcacggtaa taacactcgg
    14881 cgtcaaacga ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag
    14941 accagcaata actaagataa tccaggacat atcaggctcc ggagaattgg ggccgtcccc
    15001 ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc
    15061 aggtggcagg ggaatttcaa ctattacctt agtgactatc gcagaaaaaa caaacggcac
    15121 gacatacctg tcgtaccgtt attgcttatt gctattgctg cgctttcgtt gctgcgccag
    15181 aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag
    15241 ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc ttcaccataa
    15301 cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata atagacaaca
    15361 tcgccaaaaa tgacggaaat ctgatgattt ttcggaagag aagaaaaatt agctggcagc
    15421 gcgagagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag cggtgttaca
    15481 gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca tcatctctga
    15541 cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga
    15601 gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta cgaacagcgg
    15661 aaccttaacc acagcgccag tagacagggt agccggtttg ccgccagtac ctgcggtatc
    15721 acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg gcggagtaac
    15781 ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca gcagccattt
    15841 agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt cgttgttcat
    15901 gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga caacatcagc
    15961 gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg tcagcagacg
    16021 acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt cactcgcttc
    16081 aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat cgttgctata
    16141 gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac aaaatggcgc
    16201 atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt gccgatgttg
    16261 tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg
    16321 ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca
    16381 tggagaaagg caatccggac gatcctcttt tgcgtcaggt acttacctcg caagatgagt
    16441 ttgtcgtcgc gtccggattc tccaccgacc cgctggaaga acagcacagc gtagtgcctg
    16501 gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa
    16561 attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc aacaagcgta
    16621 actggcagac tgctcttgag tatgttgctg cgcatccgga actggacgag atgattttct
    16681 ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc acacaactgg
    16741 aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg
    16801 cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg
    16861 tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat
    16921 tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt gtgaacgata
    16981 acgcacaaac gctggcaaac ctgagtaatg cgttgttcga tgccggcgta atgccgtatt
    17041 acctgcatgt gctcgataaa gtacagggcg cggcgcattt tatggtgagt gatgacgaag
    17101 cacggcagat tatgcgtgag ttgctgacac tggtgtcggg ttatctggtg ccgaaactgg
    17161 cgcgagaaat cggcggcgaa cccagcaaaa cgccgctgga tctccagcta cgccagcagt
    17221 aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc aggccttgtg
    17281 cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt acgccgcatc
    17341 cggcaagagc aacaaacgca agtacagcaa cctgtcatta aactgggaaa tttaatcttt
    17401 gcccgttgca taacctttaa ttattcatat acattgctgt tgttactctt tatatagcca
    17461 acaccaataa ttaacctttc ttttctgttt atatagagtg agtacggcta tttatgttat
    17521 tttaacaact attactacca agcccattta tctggcttat
//
LOCUS       DAPGWD010000427         6821 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.guided.203, whole genome shotgun sequence.
ACCESSION   DAPGWD010000427 DAPGWD010000000
VERSION     DAPGWD010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.guided.203"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..367
                     /locus_tag="RHX88_004678"
     CDS             <1..367
                     /locus_tag="RHX88_004678"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV2233248.1"
                     /translation="ERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRL
                     AKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLV
                     GLHVTLLDPQMERQLVLGL"
     gene            364..816
                     /locus_tag="RHX88_004679"
     CDS             364..816
                     /locus_tag="RHX88_004679"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2233249.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            1062..2201
                     /locus_tag="RHX88_004680"
     CDS             1062..2201
                     /locus_tag="RHX88_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDV2233250.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTVTQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            2198..2812
                     /locus_tag="RHX88_004681"
     CDS             2198..2812
                     /locus_tag="RHX88_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDV2233251.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(2869..4326)
                     /gene="pepD"
                     /locus_tag="RHX88_004682"
     CDS             complement(2869..4326)
                     /gene="pepD"
                     /locus_tag="RHX88_004682"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV2233252.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4587..5045
                     /gene="gpt"
                     /locus_tag="RHX88_004683"
     CDS             4587..5045
                     /gene="gpt"
                     /locus_tag="RHX88_004683"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV2233253.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            5137..6381
                     /gene="frsA"
                     /locus_tag="RHX88_004684"
     CDS             5137..6381
                     /gene="frsA"
                     /locus_tag="RHX88_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV2233254.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6439..>6821
                     /gene="crl"
                     /locus_tag="RHX88_004685"
     CDS             6439..>6821
                     /gene="crl"
                     /locus_tag="RHX88_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV2233255.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEP"
BASE COUNT         1668 a         1650 c         1806 g         1697 t
ORIGIN      
        1 cgagcgcgac gttaacagcg aacggttgcg aaaatccgtc ggcgtggaac gcacgatggc
       61 ggaagatatc caccactggt ctgaatgtga agcgattatc gagcggctgt atccggaact
      121 tgaacgccgt ctggcaaagg taaaacctga tttactgatt gcccgccagg gggtgaaatt
      181 aaagttcgac gattttcagc aaaccactca ggagcacgtc tggccgcggc tgaataaagc
      241 tgacttaatc gccaccgcgc gtaaaacctg ggatgaacgc cgcggcgggc gcggtgtgcg
      301 actggtgggg ctgcatgtga cgttgcttga cccgcaaatg gaaagacaac tggtgctggg
      361 attatgaata acatacaaat aagagactat cagcctggcg attttcagca actatgcgct
      421 attttcatta gagcggttac gatgactgcc agtcagcatt attcaccaca acaaattgcc
      481 gcctgggcgc agattgacga atctcgctgg aaggagaaac tcgcgaaatc acaagtgcgg
      541 gttgcagtca ttaatgcaaa actggtcggc tttattacct gcgttgaaca ctatatcgat
      601 atgttatttg ttgaccctga atacacccgc cgtggggttg ccagcgcttt gttaaaacct
      661 ttgattaagt ctgaatccga acttacggtg gacgcaagca taaccgcaaa accctttttt
      721 gaacgttatg gttttcagac agttaagcag caacgcgttg aatgtcgggg agagtggttt
      781 attaattttt atatgcgtta taaacagcaa cattaaatcc agcttgcaat gaaaataacg
      841 caagcctagt atgtgcgcgt tttcttattc cacaaactgc aaggaggtaa atcatgacaa
      901 atcctttatc aatgactctt tgcagacctt tccaggatta attctttttt tcttgccctg
      961 gattcgtctg ccatttcctg attttttaat tgttactctc aataattcaa gtcgcaacac
     1021 atgcaacttg aagtatgacg agtctattta cggaatggta tatgggcaat tatattcgtc
     1081 ccttatccga tgcggtattt accatcgcat ctgatgacct gtggatcgag agtttagcga
     1141 tccaacaatt acacaccacg gcaaatttac ccaacatgca gcgcgtagtg gggatgccag
     1201 atttacatcc cggacgcggc tatccgattg gcgcagcgtt cttctctgtt ggtcgttttt
     1261 atccggcact ggtcggcaat gatatcggct gcggtatggc gctatggcaa acagatattc
     1321 tcactcgcaa atacaacgcc gataagtttg aaaagcgatt atcagatctg gatgacgttg
     1381 ctgaagaaag ctggctggag gaaaacctgc cgtcagcatt tgcacagcat ccgtggcgca
     1441 gctcgcttgg ctctatcggt ggcggcaatc atttcgctga actgcaacaa gttgatcaaa
     1501 tcatcaacgc tgaactgttt gcactggcag gtctggatgc acaacatctg caactgttgg
     1561 ttcatagcgg ctcgcggggg gtggggcagt ctattttaca gcggcatatt gcctcgttct
     1621 cgcatcatgg tttgcctcaa ggcagtgacg acgcgctacg gtatattgcg gaacatgacg
     1681 atgcgctggc gtttgcgtgt attaatcgcc agatgatcgc tttgcgcata atgcaacaag
     1741 ttaaggccac gggaagtccg gttctggatg tggcgcataa ctttgttagc gcgtgtcaaa
     1801 tcggcgatca acagggctgg ttgcatcgta aaggtgccac gccggatgac aacggtctgg
     1861 tgattattcc cggttcacgt ggtgattact cctggattgt acagcctgtt gcgaacgaaa
     1921 aaacgctcca ctcgctggcg catggggcag ggcgtaaatg ggggcgcacc gagtgtaaag
     1981 ggcgtctggc agcgaaatac acggtgacgc aactctcgcg gactgaactt ggcagtcggg
     2041 taatttgtcg cgataaacaa ctcatctttg aagaagcgcc acaagcctat aaatcggctg
     2101 aaagcgtggt gcaatgtctg gtgcaggccg gtttaattat tcctgtcgcg cggctgcgtc
     2161 cggtgctaac gctcaaaaac agtggaggga aaaaaggatg atcttgctac aactctcctc
     2221 agcgcagggg ccggaagaat gttgtctcgc agtgaaaaag gcgctggagc gattaattaa
     2281 agaagctgcc cgacaagacg tcgcggtaac ggtgttggaa acagaaacgg gccgctactc
     2341 tgacacgctg cgttcagctc tggtttctct ggatggcgat aacgcatggg cattaagcga
     2401 aagctggtgc ggcactattc agtggatttg tccgagtccg tatcggcctc atcatgggcg
     2461 caaaaactgg tttctgggca ttgggcgttt taccgctgat gagcaggaac aatcggatgc
     2521 aatccgttat gagacgttgc gttcgtcggg gccgggcggt caacatgtca ataaaaccga
     2581 ctcggcggta cgcgccacgc atttggcatc cggtattagc gtgaaggttc agtcagagcg
     2641 tagtcagcat gctaacaagc ggctggcacg attgctgatt gcctggaagc tggagcagca
     2701 gcaacaggaa aatagcgcgg tgctgaaatc gcagcggcga atgttccatc accagattga
     2761 acgtggcaac ccgagacgga catttacagg gatggctttt atcgaaggat aatgaaggat
     2821 gaaactgccg gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg
     2881 aatttctttc agcagttcag tcagcagcgt ccagtaatga cctacgcttt cgatgtgaac
     2941 ttgctcatcc ggagagtgtg gaccggtgat agttggcccg atagaaacca tgtccatttc
     3001 cggatacggt tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg
     3061 tgttttgttg aacagacgct gataggtttc acgtaccaga tgcatcaccg gagaattagc
     3121 gtccggctgc cagccaggat atgcgccttt cgcttcggtt ttcgcgccag ctagtttacc
     3181 cagcgaatcc agcatgctca ccacgtagtc tttaccgctg tcgatcagtg aacggatcag
     3241 gcagtgaatt tctacgttat tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc
     3301 gaccacacct tttgccacgt cggaattgcg gatcacaccg ttcggggtgg cgttcagcag
     3361 acgaataaag gtatcgcgag atttcgcaat cagggcagct ttatcgttcg ctacagagtc
     3421 cagcaacaag gccagatttt tctctttctc tgccagctcg tttttcagga tctcctgata
     3481 ggtattcacc agagatttca ggacgtcgac tttatcagct gcgacagcaa tggtcgcaaa
     3541 ggcttcacgc gggatggcgt tacgcagtgt gccgccgttg aaatcgataa ggcgcagatc
     3601 cagttcttcc gcatgacccg ccaggaagcg caccagtagt ttgttggcat tacccaggcc
     3661 aacgtggatt tcaccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt
     3721 ttcaaaacca gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc
     3781 ccccgcacaa cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc
     3841 agcctgcaac cagttgctct gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat
     3901 ggtcagcagc acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagcgc
     3961 agacgccata ccaatgccgt tatctgcacc cagcgtggtg ccgcgcgctt taacccattc
     4021 gccatcaata taaggctgga taggatcttt cgtgaagtca tgcacggtgt cgttattttt
     4081 ctgcggcacc atatcgaggt gggcctgtaa gacgaccggt ttacgatttt ccatacctgc
     4141 ggtagcaggt ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt
     4201 ctcttttgcc caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg
     4261 aatagaacag attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc
     4321 agacacgtta agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag
     4381 cagaaaatgt tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     4441 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     4501 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     4561 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     4621 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     4681 ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta
     4741 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     4801 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
     4861 ataccggtgg tactgctgtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
     4921 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
     4981 atacctggat cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
     5041 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcaggtta
     5101 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
     5161 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
     5221 cacggcgcac aaccgcctgt gcagtcggct cttgatggta aaaccatccc tcactggtat
     5281 cgcatgatta accgtctgat gtggatctgg cgcggtattg acccacgtga aatcctcgac
     5341 gtccaggcac gtattgtgat gagcgatgct gaacgtaccg acgatgattt atacgatact
     5401 gtgattggct accgtggtgg caactggatt tatgagtggg ccacccaggc gatggtgtgg
     5461 caacaaaaag cctgtgcgga agaagatccg caactcagtg gtcgacactg gctgcatgcg
     5521 gctacgttgt acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa
     5581 gcgcaggctt tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc gggcacgatg
     5641 cggcagatgg agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg
     5701 aaaggcgatg gcccgttccc gacagtatta atgtgtggtg gtctggatgc gatgcagacg
     5761 gactattaca gcctgtatga acgttatttt gcgccgcgcg gcattgcgat gctgactatt
     5821 gatatgccat cggtgggctt ttcttcaaaa tggaagctca cccaggactc cagcctgttg
     5881 catcagcacg tcttaaaggc gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg
     5941 gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg
     6001 cgtctgaaag cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag
     6061 tgccagcaac aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat
     6121 gatgcttccg atgacgcgtt acgcgtggag ctgaatcgct attcattaaa agtgcaagga
     6181 ttgctggggc gtcgctgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc
     6241 agcccggaag aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag
     6301 atcccattta acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccggctgg
     6361 atcgaaaaac gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgca
     6421 tcacaacagg agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa
     6481 aaatttaccg cactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt
     6541 ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg
     6601 ggctggtgga tggagcttga agcgcaggaa tcccgtttta cctacagtta ccagtttggt
     6661 ctgttcgata aagcaggcga ctggaagagt gttccggtaa aagacactga agtggttgaa
     6721 cgactggagc acaccctgcg tgagtttcac gagaagctgc gtgaactgct gacgacgctg
     6781 aatctgaagc tggaaccggc ggatgatttt cgtgacgagc c
//
LOCUS       DAPGWD010000428         3118 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.guided.265, whole genome shotgun sequence.
ACCESSION   DAPGWD010000428 DAPGWD010000000
VERSION     DAPGWD010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.guided.265"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            292..1437
                     /locus_tag="RHX88_004686"
     CDS             292..1437
                     /locus_tag="RHX88_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV2233256.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            1531..2064
                     /locus_tag="RHX88_004687"
     CDS             1531..2064
                     /locus_tag="RHX88_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV2233257.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(2061..2378)
                     /gene="sugE"
                     /locus_tag="RHX88_004688"
     CDS             complement(2061..2378)
                     /gene="sugE"
                     /locus_tag="RHX88_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV2233258.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
     gene            2430..2900
                     /locus_tag="RHX88_004689"
                     /pseudo
     CDS             2430..2900
                     /locus_tag="RHX88_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          832 a          759 c          780 g          747 t
ORIGIN      
        1 ctgtggataa cttgcagagt ttattaagta tcattgcagc aaagatgaaa tcaatgattt
       61 atcaaaaatg attgaaaggt ggttgtaaat aatgttacaa tgtgtgagaa gcagtctaaa
      121 ttcttcgtga aatagtgatt tttgaagcta ataaaaaaca cacgtggaat ttaggaaaaa
      181 cttatatctg ctgctaaatt taaccgtttg tcaacacggt gcaaatcaaa cacactgatt
      241 gcgtctgacg ggcccggaca cctttttgct tttaattacg gaactgattt catgatgaaa
      301 aaatcgttat gctgcgctct gctgctgaca gcctctttct ccacatttgc tgccgcaaaa
      361 acagaacaac agattgccga tatcgttaat cgcaccatca ccccgttgat gcaggagcag
      421 gctattccgg gtatggccgt tgccgttatc taccagggaa aaccctatta tttcacctgg
      481 ggtaaagccg atatcgccaa taaccaccca gtcacgcagc aaacgctgtt tgagctagga
      541 tcggttagta agacgtttaa cggcgtgttg ggcggcgatg ctatcgcccg cggcgaaatt
      601 aagctcagcg atccggtcac gaaatactgg ccagaactga caggcaaaca gtggcagggt
      661 atccgcctgc tgcacttagc cacctatacg gcaggcggcc taccgctgca gatccccgat
      721 gacgttaggg ataaagccgc attactgcat ttttatcaaa actggcagcc gcaatggact
      781 ccgggcgcta agcgacttta cgctaactcc agcattggtc tgtttggcgc gctggcggtg
      841 aaaccctcag gaatgagtta cgaagaggca atgaccagac gcgtcctgca accattaaaa
      901 ctggcgcata cctggattac ggttccgcag aacgaacaaa aagattatgc ctggggctat
      961 cgcgaaggga agcccgtaca cgtttctccg ggacaacttg acgccgaagc ctatggcgtg
     1021 aaatccagcg ttattgatat ggcccgctgg gttcaggcca acatggatgc cagccacgtt
     1081 caggagaaaa cgctccagca gggcattgcg cttgcgcagt ctcgctactg gcgtattggc
     1141 gatatgtacc agggattagg ctgggagatg ctgaactggc cgctgaaagc tgattcgatc
     1201 atcaacggca gcgacagcaa agtggcattg gcagcgcttc ccgccgttga ggtaaacccg
     1261 cccgcccccg cagtgaaagc ctcatgggtg cataaaacgg gctccactgg tggatttggc
     1321 agctacgtag ccttcgttcc agaaaaaaac cttggcatcg tgatgctggc aaacaaaagc
     1381 tatcctaacc ctgtccgtgt cgaggcggcc tggcgcattc ttgaaaagct gcaataactg
     1441 acgatgaggc ccaggatatt gggcctcctt tctttctctt tttttcctgt tgtcatctac
     1501 acttaacaaa aatacagcaa ggaaaatccc atgcgcattt tgcccgtcgt tgctgcagtt
     1561 acggctgcat tcctggttgt cgcgtgtagc tccccgacac cgccgaaagg cgttaccgtg
     1621 gtaaataact ttgatgccaa acgctatctg ggaacctggt atgaaattgc gcgcttcgac
     1681 catcgtttcg agcgcggatt ggataaagtg accgcaacat acagcttgcg cgacgacggc
     1741 ggcatcaacg ttattaacaa gggctataac cctgacaggg agatgtggca gaaaacggaa
     1801 gggaaagcct atttcaccgg cgacccaagc agagccgcgc ttaaggtttc ttttttcggc
     1861 cccttctatg gcgggtataa cgtaattgca ctcgaccggg aatatcgtca cgcgctggtt
     1921 tgtggtccgg atcgcgacta cctgtggatc ctttcacgga cccctactat ttcagatgaa
     1981 atgaaacagc aaatgttagc catcgcgacc cgggaagggt ttgaagtgaa taaactgatt
     2041 tgggtgaaac agcctggcgc ttagtgagtg ctcagcttca gaccaataat gccagcaacg
     2101 atcagcccaa ggctcagcaa acgtgccggg ctggcagact cacccagcag caaaatccct
     2161 gtaatggccg ccccaacagc gccaataccg gtccagaccg cataagcggt tcctacaggc
     2221 aacgtgcgca ttgcccaaga gagcatggcg atactgacga tcatcgccgc aatagtgata
     2281 atgcttggcg taagacgcgt aaaaccgtgg gtgtatttca ggccaatcgc ccagacaact
     2341 tcgagcaaac ctgcaattaa taaaacgatc caggacatat caggctccag aacaatgggg
     2401 ccgtccccgg tgaaagaagc gtttgcaggt gggttattct tgctttttgc cggagtgccg
     2461 atggcagaat catctgcagt gaagggtatg ctcatgctct ttatcaactg acatgtccgg
     2521 tgcctgtgga cagcaaactg gaacgaaaca cgctcactgc tcttctgaat gtggccagct
     2581 ggcttaaaag aaagccaggt acgccggaat taagtctgga aaggcccctg tttgatacag
     2641 aagtttatgt taatggtgaa aagaaatatg tactgccgga tttcattgtc acagcaaggg
     2701 ctcctgacgg aaagacggcc agagtggtca tcgaaacgat gggatatgaa gacagtgatt
     2761 actgcgcgag aaaatccagg cagcataccg gcatgaagca gattggtgtt ctgcataccg
     2821 atccaccgaa atggctggat aacgatcatc ccccttttaa gaaacatatg tacggtgttt
     2881 ttatgcatct caggtactga gatattttgt ggctcagttc tgtaactttt cccgtaacat
     2941 tgtctgttgt tacgggaaag tccggttttt gtattgcacc agagaatacc cagactgtga
     3001 tgctgccaca gtgtcagcag gctttctgaa cggtataaca ccacttcatg ttaatgataa
     3061 ttactatcat taaatcttga catgccattt tctccttaat aaattagtac tgtatatg
//
LOCUS       DAPGWD010000429         1714 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16509
            SAMN37211263-rid19352963.guided.318, whole genome shotgun sequence.
ACCESSION   DAPGWD010000429 DAPGWD010000000
VERSION     DAPGWD010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211263
            Sequence Read Archive: SRR25824930
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875654.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875654.1
            Genome Coverage       :: 57x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,622
            Genes (coding)                    :: 4,523
            CDSs (with protein)               :: 4,523
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 48 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 18 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211263-rid19352963.guided.318"
                     /isolate="ARS-CC16509"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            103..1308
                     /gene="tet(B)"
                     /locus_tag="RHX88_004690"
     CDS             103..1308
                     /gene="tet(B)"
                     /locus_tag="RHX88_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDV2233259.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLNNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1421..>1714)
                     /locus_tag="RHX88_004691"
     CDS             complement(1421..>1714)
                     /locus_tag="RHX88_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2233260.1"
                     /translation="IDNNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQE
                     NQLKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          430 a          314 c          374 g          596 t
ORIGIN      
        1 acttttatct aatctagaca tcattaattc ctaatttttg ttgacactct atcattgata
       61 gagttatttt accactccct atcagtgata gagaaaagtg aaatgaatag ttcgacaaag
      121 atcgcattgg taattacgtt actcgatgcc atggggattg gccttatcat gccagtcttg
      181 ccaacgttat tacgtgaatt tattgcttcg gaagatatcg ctaaccactt tggcgtattg
      241 cttgcacttt atgcgttaat gcaggttatc tttgctcctt ggcttggaaa aatgtctgac
      301 cgatttggtc ggcgcccagt gctgttgttg tcattaatag gcgcatcgct ggattactta
      361 ttgctggctt tttcaagtgc gctttggatg ctgtatttag gccgtttgct ttcagggatc
      421 acaggagcta ctggggctgt cgcggcatcg gtcattgccg ataccacctc agcttctcaa
      481 cgcgtgaagt ggttcggttg gttaggggca agttttgggc ttggtttaat agcggggcct
      541 attattggtg gttttgcagg agagatttca ccgcatagtc ccttttttat cgctgcgttg
      601 ctaaatattg tcgctttcct tgtggttatg ttttggttcc gtgaaaccaa aaatacacgt
      661 gataatacag ataccgaagt aggggttgag acgcaatcga attcggtata catcacttta
      721 tttaaaacga tgcccatttt gttgattatt tatttttcag cgcaattgat aggccaaatt
      781 cccgcaacgg tgtgggtgct atttaccgaa aatcgttttg gatggaatag catgatggtt
      841 ggcttttcat tagcgggtct tggtctttta cactcagtat tccaagcctt tgtggcagga
      901 agaatagcca ctaaatgggg cgaaaaaacg gcagtactgc tcggatttat tgcagatagt
      961 agtgcatttg cctttttagc gtttatatct gaaggttggt tagttttccc tgttttaatt
     1021 ttattggctg gtggtgggat cgctttacct gcattacagg gagtgatgtc tatccaaaca
     1081 aagagtcatc agcaaggtgc tttacaggga ttattggtga gccttaacaa tgcaaccggt
     1141 gttattggcc cattactgtt tgctgttatt tataatcatt cactaccaat ttgggatggc
     1201 tggatttgga ttattggttt agcgttttac tgtattatta tcctgctatc gatgaccttc
     1261 atgttaaccc ctcaagctca ggggagtaaa caggagacaa gtgcttagtt atttcgtcac
     1321 caaatgatgt tattccgcga aatataatga ccctcttgat aacccaagag ggcatttttt
     1381 acgataaaga agatttagct tcaaataaaa cctatctatt ttatttatct ttcaagctca
     1441 ataaaaagcc gcggtaaata gcaataaatt ggcctttttt atcggcaagc tcttttaggt
     1501 ttttcgcatg tattgcgata tgcataaacc agccattgag taagttttta agcacatcat
     1561 catcataagc tttaagttgg ttctcttgga tcaatttgct gacaatggcg tttaccttac
     1621 cagtaatgta ttcaaggcta attttttcaa gttcattcca accaatgata ggcatcactt
     1681 cttggatagg gataaggttt ttattattat caat
//
